LOCUS       DAZFJF010000001       114490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFJF010000001 DAZFJF010000000
VERSION     DAZFJF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 114490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.001"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..596)
                     /locus_tag="ACHKAU_000001"
     CDS             complement(<1..596)
                     /locus_tag="ACHKAU_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5529142.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKAU_000002"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKAU_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO5529143.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKAU_000003"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKAU_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO5529144.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKAU_000004"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKAU_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO5529145.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKAU_000005"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKAU_000005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO5529146.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKAU_000006"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKAU_000006"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO5529147.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKAU_000007"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKAU_000007"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO5529148.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKAU_000008"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKAU_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5529149.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKAU_000009"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKAU_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5529150.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKAU_000010"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKAU_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO5529151.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKAU_000011"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKAU_000011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5529152.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKAU_000012"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKAU_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO5529153.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKAU_000013"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKAU_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO5529154.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKAU_000014"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKAU_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO5529155.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKAU_000015"
     CDS             10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKAU_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5529156.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKAU_000016"
     CDS             12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKAU_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5529157.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKAU_000017"
     CDS             13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKAU_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO5529158.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKAU_000018"
     CDS             13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKAU_000018"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO5529159.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKAU_000019"
     CDS             complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKAU_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO5529160.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="ACHKAU_000020"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="ACHKAU_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO5529161.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKAU_000021"
     CDS             16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKAU_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO5529162.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKAU_000022"
                     /gene_synonym="dapC"
     CDS             complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKAU_000022"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO5529163.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKAU_000023"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKAU_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO5529164.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKAU_000024"
     CDS             complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKAU_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO5529165.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKAU_000025"
     CDS             complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKAU_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO5529166.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKAU_000026"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKAU_000026"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO5529167.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKAU_000027"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKAU_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO5529168.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSSHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKAU_000028"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKAU_000028"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO5529169.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWCQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKAU_000029"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKAU_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO5529170.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26270..27076
                     /gene="nirC"
                     /locus_tag="ACHKAU_000030"
     CDS             26270..27076
                     /gene="nirC"
                     /locus_tag="ACHKAU_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO5529171.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLSNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27095..28468
                     /gene="cysG"
                     /locus_tag="ACHKAU_000031"
     CDS             27095..28468
                     /gene="cysG"
                     /locus_tag="ACHKAU_000031"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO5529172.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28715..28882
                     /gene="yhfL"
                     /locus_tag="ACHKAU_000032"
     CDS             28715..28882
                     /gene="yhfL"
                     /locus_tag="ACHKAU_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO5529173.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29177..30514
                     /gene="frlA"
                     /locus_tag="ACHKAU_000033"
     CDS             29177..30514
                     /gene="frlA"
                     /locus_tag="ACHKAU_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO5529174.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30535..31557
                     /gene="frlB"
                     /locus_tag="ACHKAU_000034"
     CDS             30535..31557
                     /gene="frlB"
                     /locus_tag="ACHKAU_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO5529175.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31607..32437
                     /gene="frlC"
                     /locus_tag="ACHKAU_000035"
     CDS             31607..32437
                     /gene="frlC"
                     /locus_tag="ACHKAU_000035"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO5529176.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32434..33219
                     /gene="frlD"
                     /locus_tag="ACHKAU_000036"
     CDS             32434..33219
                     /gene="frlD"
                     /locus_tag="ACHKAU_000036"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO5529177.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33319..34050
                     /gene="frlR"
                     /locus_tag="ACHKAU_000037"
                     /gene_synonym="yhfR"
     CDS             33319..34050
                     /gene="frlR"
                     /locus_tag="ACHKAU_000037"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO5529178.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34202..35287)
                     /gene="yhfS"
                     /locus_tag="ACHKAU_000038"
     CDS             complement(34202..35287)
                     /gene="yhfS"
                     /locus_tag="ACHKAU_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5529179.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35299..36603)
                     /gene="yhfT"
                     /locus_tag="ACHKAU_000039"
     CDS             complement(35299..36603)
                     /gene="yhfT"
                     /locus_tag="ACHKAU_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO5529180.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36615..36968)
                     /gene="yhfU"
                     /locus_tag="ACHKAU_000040"
     CDS             complement(36615..36968)
                     /gene="yhfU"
                     /locus_tag="ACHKAU_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO5529181.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36979..37857)
                     /gene="php"
                     /locus_tag="ACHKAU_000041"
     CDS             complement(36979..37857)
                     /gene="php"
                     /locus_tag="ACHKAU_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO5529182.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37854..39080)
                     /gene="yhfW"
                     /locus_tag="ACHKAU_000042"
     CDS             complement(37854..39080)
                     /gene="yhfW"
                     /locus_tag="ACHKAU_000042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5529183.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(39080..40243)
                     /gene="yhfX"
                     /locus_tag="ACHKAU_000043"
     CDS             complement(39080..40243)
                     /gene="yhfX"
                     /locus_tag="ACHKAU_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO5529184.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(40327..40689)
                     /gene="yhfY"
                     /locus_tag="ACHKAU_000044"
     CDS             complement(40327..40689)
                     /gene="yhfY"
                     /locus_tag="ACHKAU_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5529185.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40706..41611)
                     /gene="yhfZ"
                     /locus_tag="ACHKAU_000045"
     CDS             complement(40706..41611)
                     /gene="yhfZ"
                     /locus_tag="ACHKAU_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO5529186.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41880..42884)
                     /gene="trpS"
                     /locus_tag="ACHKAU_000046"
     CDS             complement(41880..42884)
                     /gene="trpS"
                     /locus_tag="ACHKAU_000046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO5529187.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42877..43635)
                     /gene="gph"
                     /locus_tag="ACHKAU_000047"
     CDS             complement(42877..43635)
                     /gene="gph"
                     /locus_tag="ACHKAU_000047"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO5529188.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43628..44305)
                     /gene="rpe"
                     /locus_tag="ACHKAU_000048"
     CDS             complement(43628..44305)
                     /gene="rpe"
                     /locus_tag="ACHKAU_000048"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO5529189.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44323..45159)
                     /gene="dam"
                     /locus_tag="ACHKAU_000049"
     CDS             complement(44323..45159)
                     /gene="dam"
                     /locus_tag="ACHKAU_000049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5529190.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45266..46552)
                     /gene="damX"
                     /locus_tag="ACHKAU_000050"
     CDS             complement(45266..46552)
                     /gene="damX"
                     /locus_tag="ACHKAU_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO5529191.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46644..47732)
                     /gene="aroB"
                     /locus_tag="ACHKAU_000051"
     CDS             complement(46644..47732)
                     /gene="aroB"
                     /locus_tag="ACHKAU_000051"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO5529192.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            complement(47789..48310)
                     /gene="aroK"
                     /locus_tag="ACHKAU_000052"
     CDS             complement(47789..48310)
                     /gene="aroK"
                     /locus_tag="ACHKAU_000052"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO5529193.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48711..49949)
                     /gene="hofQ"
                     /locus_tag="ACHKAU_000053"
     CDS             complement(48711..49949)
                     /gene="hofQ"
                     /locus_tag="ACHKAU_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO5529194.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49861..50265)
                     /gene="hofP"
                     /locus_tag="ACHKAU_000054"
     CDS             complement(49861..50265)
                     /gene="hofP"
                     /locus_tag="ACHKAU_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO5529195.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50255..50695)
                     /gene="hofO"
                     /locus_tag="ACHKAU_000055"
     CDS             complement(50255..50695)
                     /gene="hofO"
                     /locus_tag="ACHKAU_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO5529196.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50679..51218)
                     /gene="hofN"
                     /locus_tag="ACHKAU_000056"
     CDS             complement(50679..51218)
                     /gene="hofN"
                     /locus_tag="ACHKAU_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO5529197.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51218..51997)
                     /gene="hofM"
                     /locus_tag="ACHKAU_000057"
     CDS             complement(51218..51997)
                     /gene="hofM"
                     /locus_tag="ACHKAU_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO5529198.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            52117..54669
                     /gene="mrcA"
                     /locus_tag="ACHKAU_000058"
     CDS             52117..54669
                     /gene="mrcA"
                     /locus_tag="ACHKAU_000058"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO5529199.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54835..55395)
                     /gene="nudE"
                     /locus_tag="ACHKAU_000059"
     CDS             complement(54835..55395)
                     /gene="nudE"
                     /locus_tag="ACHKAU_000059"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO5529200.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55715..57850
                     /gene="igaA"
                     /locus_tag="ACHKAU_000060"
     CDS             55715..57850
                     /gene="igaA"
                     /locus_tag="ACHKAU_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO5529201.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57915..58583
                     /gene="yrfG"
                     /locus_tag="ACHKAU_000061"
     CDS             57915..58583
                     /gene="yrfG"
                     /locus_tag="ACHKAU_000061"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO5529202.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58594..58995
                     /gene="hslR"
                     /locus_tag="ACHKAU_000062"
     CDS             58594..58995
                     /gene="hslR"
                     /locus_tag="ACHKAU_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO5529203.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            59020..59898
                     /gene="hslO"
                     /locus_tag="ACHKAU_000063"
     CDS             59020..59898
                     /gene="hslO"
                     /locus_tag="ACHKAU_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO5529204.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(60033..61757)
                     /gene="yhgE"
                     /locus_tag="ACHKAU_000064"
     CDS             complement(60033..61757)
                     /gene="yhgE"
                     /locus_tag="ACHKAU_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO5529205.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            62136..63758
                     /gene="pckA"
                     /locus_tag="ACHKAU_000065"
     CDS             62136..63758
                     /gene="pckA"
                     /locus_tag="ACHKAU_000065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO5529206.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63834..65186)
                     /gene="envZ"
                     /locus_tag="ACHKAU_000066"
     CDS             complement(63834..65186)
                     /gene="envZ"
                     /locus_tag="ACHKAU_000066"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO5529207.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(65183..65902)
                     /gene="ompR"
                     /locus_tag="ACHKAU_000067"
     CDS             complement(65183..65902)
                     /gene="ompR"
                     /locus_tag="ACHKAU_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO5529208.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            66130..66606
                     /gene="greB"
                     /locus_tag="ACHKAU_000068"
     CDS             66130..66606
                     /gene="greB"
                     /locus_tag="ACHKAU_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO5529209.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66703..69024
                     /gene="yhgF"
                     /locus_tag="ACHKAU_000069"
     CDS             66703..69024
                     /gene="yhgF"
                     /locus_tag="ACHKAU_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO5529210.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69462..69689
                     /gene="feoA"
                     /locus_tag="ACHKAU_000070"
     CDS             69462..69689
                     /gene="feoA"
                     /locus_tag="ACHKAU_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO5529211.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69706..72027
                     /gene="feoB"
                     /locus_tag="ACHKAU_000071"
     CDS             69706..72027
                     /gene="feoB"
                     /locus_tag="ACHKAU_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO5529212.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            72027..72263
                     /gene="feoC"
                     /locus_tag="ACHKAU_000072"
     CDS             72027..72263
                     /gene="feoC"
                     /locus_tag="ACHKAU_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO5529213.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72466..73344
                     /gene="rpnA"
                     /locus_tag="ACHKAU_000073"
     CDS             72466..73344
                     /gene="rpnA"
                     /locus_tag="ACHKAU_000073"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO5529214.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(73373..74143)
                     /gene="bioH"
                     /locus_tag="ACHKAU_000074"
     CDS             complement(73373..74143)
                     /gene="bioH"
                     /locus_tag="ACHKAU_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO5529215.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            74181..74864
                     /gene="gntX"
                     /locus_tag="ACHKAU_000075"
     CDS             74181..74864
                     /gene="gntX"
                     /locus_tag="ACHKAU_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO5529216.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74923..75498
                     /gene="nfuA"
                     /locus_tag="ACHKAU_000076"
     CDS             74923..75498
                     /gene="nfuA"
                     /locus_tag="ACHKAU_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO5529217.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75859..77175
                     /gene="gntT"
                     /locus_tag="ACHKAU_000077"
     CDS             75859..77175
                     /gene="gntT"
                     /locus_tag="ACHKAU_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5529218.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMAQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77220..79304)
                     /gene="malQ"
                     /locus_tag="ACHKAU_000078"
     CDS             complement(77220..79304)
                     /gene="malQ"
                     /locus_tag="ACHKAU_000078"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO5529219.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79314..81707)
                     /gene="malP"
                     /locus_tag="ACHKAU_000079"
     CDS             complement(79314..81707)
                     /gene="malP"
                     /locus_tag="ACHKAU_000079"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO5529220.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            82319..85024
                     /gene="malT"
                     /locus_tag="ACHKAU_000080"
     CDS             82319..85024
                     /gene="malT"
                     /locus_tag="ACHKAU_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO5529221.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(85067..86083)
                     /gene="rtcA"
                     /locus_tag="ACHKAU_000081"
     CDS             complement(85067..86083)
                     /gene="rtcA"
                     /locus_tag="ACHKAU_000081"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO5529222.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(86087..87313)
                     /gene="rtcB"
                     /locus_tag="ACHKAU_000082"
     CDS             complement(86087..87313)
                     /gene="rtcB"
                     /locus_tag="ACHKAU_000082"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO5529223.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            87502..89100
                     /gene="rtcR"
                     /locus_tag="ACHKAU_000083"
     CDS             87502..89100
                     /gene="rtcR"
                     /locus_tag="ACHKAU_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO5529224.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(89082..89840)
                     /locus_tag="ACHKAU_000084"
     CDS             complement(89082..89840)
                     /locus_tag="ACHKAU_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO5529225.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(89857..90687)
                     /gene="glpG"
                     /locus_tag="ACHKAU_000085"
     CDS             complement(89857..90687)
                     /gene="glpG"
                     /locus_tag="ACHKAU_000085"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO5529226.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(90732..91058)
                     /gene="glpE"
                     /locus_tag="ACHKAU_000086"
     CDS             complement(90732..91058)
                     /gene="glpE"
                     /locus_tag="ACHKAU_000086"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO5529227.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            91248..92753
                     /gene="glpD"
                     /locus_tag="ACHKAU_000087"
     CDS             91248..92753
                     /gene="glpD"
                     /locus_tag="ACHKAU_000087"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO5529228.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(92805..93410)
                     /locus_tag="ACHKAU_000088"
     CDS             complement(92805..93410)
                     /locus_tag="ACHKAU_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529229.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(93413..94180)
                     /locus_tag="ACHKAU_000089"
     CDS             complement(93413..94180)
                     /locus_tag="ACHKAU_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529230.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(94183..94893)
                     /locus_tag="ACHKAU_000090"
     CDS             complement(94183..94893)
                     /locus_tag="ACHKAU_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529231.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(94909..96417)
                     /locus_tag="ACHKAU_000091"
     CDS             complement(94909..96417)
                     /locus_tag="ACHKAU_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5529232.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(96543..98990)
                     /gene="glgP"
                     /locus_tag="ACHKAU_000092"
     CDS             complement(96543..98990)
                     /gene="glgP"
                     /locus_tag="ACHKAU_000092"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO5529233.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(99009..100442)
                     /gene="glgA"
                     /locus_tag="ACHKAU_000093"
     CDS             complement(99009..100442)
                     /gene="glgA"
                     /locus_tag="ACHKAU_000093"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO5529234.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(100442..101737)
                     /gene="glgC"
                     /locus_tag="ACHKAU_000094"
     CDS             complement(100442..101737)
                     /gene="glgC"
                     /locus_tag="ACHKAU_000094"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO5529235.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(101755..103728)
                     /gene="glgX"
                     /locus_tag="ACHKAU_000095"
     CDS             complement(101755..103728)
                     /gene="glgX"
                     /locus_tag="ACHKAU_000095"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO5529236.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(103725..105911)
                     /gene="glgB"
                     /locus_tag="ACHKAU_000096"
     CDS             complement(103725..105911)
                     /gene="glgB"
                     /locus_tag="ACHKAU_000096"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO5529237.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(106184..107287)
                     /gene="asd"
                     /locus_tag="ACHKAU_000097"
     CDS             complement(106184..107287)
                     /gene="asd"
                     /locus_tag="ACHKAU_000097"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5529238.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKVLDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            107479..108072
                     /gene="yhgN"
                     /locus_tag="ACHKAU_000098"
     CDS             107479..108072
                     /gene="yhgN"
                     /locus_tag="ACHKAU_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO5529239.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(108129..109469)
                     /gene="gntU"
                     /locus_tag="ACHKAU_000099"
     CDS             complement(108129..109469)
                     /gene="gntU"
                     /locus_tag="ACHKAU_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5529240.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(109473..110000)
                     /gene="gntK"
                     /locus_tag="ACHKAU_000100"
     CDS             complement(109473..110000)
                     /gene="gntK"
                     /locus_tag="ACHKAU_000100"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5529241.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(110139..111134)
                     /gene="gntR"
                     /locus_tag="ACHKAU_000101"
     CDS             complement(110139..111134)
                     /gene="gntR"
                     /locus_tag="ACHKAU_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO5529242.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(111358..112053)
                     /gene="yhhW"
                     /locus_tag="ACHKAU_000102"
     CDS             complement(111358..112053)
                     /gene="yhhW"
                     /locus_tag="ACHKAU_000102"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO5529243.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(112176..113213)
                     /gene="yhhX"
                     /locus_tag="ACHKAU_000103"
     CDS             complement(112176..113213)
                     /gene="yhhX"
                     /locus_tag="ACHKAU_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5529244.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            113546..114034
                     /gene="yhhY"
                     /locus_tag="ACHKAU_000104"
     CDS             113546..114034
                     /gene="yhhY"
                     /locus_tag="ACHKAU_000104"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5529245.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            114271..>114490
                     /gene="tssD"
                     /locus_tag="ACHKAU_000105"
     CDS             114271..>114490
                     /gene="tssD"
                     /locus_tag="ACHKAU_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO5529246.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLF"
BASE COUNT        27639 a        31099 c        28798 g        26954 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga aaacggctcg tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcgataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa tcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat ccccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacgtcgc tcatggggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtga tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcaaacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    12001 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagctga gctgcgtgcg caaacccagc cactgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt
    12541 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa
    12661 aaccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12721 ctcaacagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14941 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtttgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15841 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaattggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16381 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcacgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcagatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttctgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgtacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa aaggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cggactatga cgcacacagg
    19081 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcat
    19681 gctggtaatg agccaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccgggtgat atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagcg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatac ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccccg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg tggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22141 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 agtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactctc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccgggcg caccgttttt
    23581 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcact attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 ggtatcgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag
    24181 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24421 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24481 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggttactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggtg tcagctgatt
    25141 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
    25561 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacaccg
    25681 tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggcg acgagcaggt cgcgattttc cgcccgtatc
    25921 acagcgacca ggtgtttgcg atcagcaaca tcgatccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtataatgc aggacatttt
    26221 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    26281 cactattaat aagtgtgcgg ctaatgctgc acgcattgca cgcctgtcgg caaataaccc
    26341 gctcggcttt tgggtcagct ccgccatggc gggcgcatat gtgggtcttg ggatcatcct
    26401 gattttcacg ctcagtaatt tgctcgaccc gtccgtacgt ccgctggtga tgggcgcgac
    26461 ctttggtatc gccttaacgc tggtgattat cgccggttct gaactgttca ccggacacac
    26521 catgttcctc acctttgggg taaaagcggg cagcatcagc cacgggcaaa tgtgggcaat
    26581 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    26641 ctggggcggc ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    26701 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    26761 ttgcctggca atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgctatctg
    26821 gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac tctatcgcta acatgacgct
    26881 gttcgcgctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    26941 taacctgctg tgggtgacgc tgggtaatac tttatcaggt gccgtattca tgggattggg
    27001 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    27061 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    27121 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    27181 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    27241 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    27301 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacacgctta accagcgcgt
    27361 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta gtcgatgcgc cgaaagccgc
    27421 cagctttatt atgccgtcga ttattgaccg ctcaccgcta atggtagcgg tctcctctgg
    27481 cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgtt
    27541 gcatctgggc caggtggcga aatatgccgg gcaattacgc gggcgagtga aacaacagtt
    27601 cgccacgatg agtgagcgtc gccgtttctg ggagaaattg ttcgttaacg accgcctggc
    27661 gcagtcgctg gcaaacaacg atcagaaagc cattactgaa acgaccgaac agttaatcaa
    27721 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    27781 gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    27841 tctggtttct gacgatatta tgaatctggt acgccgcgat gctgatcgcg ttttcgtcgg
    27901 caaacgcgcg ggataccact gtgtaccgca ggaagagatt aaccagatcc tgctgcggga
    27961 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc gatccgttta tttttggccg
    28021 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    28081 tattaccgca gcttctggtt gctctgccta ttcgggtatt ccgctcacgc atcgcgatta
    28141 tgcccagagc gtgcgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    28201 aaacctggcg gcagaaaaac agacgctggt gttctatatg gggctgaatc aggctgcgac
    28261 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtgg caattgtcga
    28321 aaacggaaca gcagtcacgc agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact
    28381 tgctcagcaa atgaacagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    28441 taaactgaac tggttctcca accattaatt taacccgggc cagagaattc tggccttctt
    28501 aatgactctt tttatatatt cagcaaataa aacatagccc cttaataaat attatctgct
    28561 aatgggttat attcaccgtt acagcgactt acaaaattaa gtcattcgct cactgacgcc
    28621 aattaaatat tcctgtatcg ttcttaacga gcagtctgct tatattcata taatcaatga
    28681 atattaatta atatataata catagggaat gtaaatgaac aaatttatta aagttgcact
    28741 ggtaggtgca gtactggcta cgttaactgc atgtactggt catattgaaa accgtgataa
    28801 gaactgctct tacgactacc tgctgcaccc ggcaatttct atttctaaaa tcattggcgg
    28861 ttgcggtcct actgcacagt aagtctctga cagaaatcgg ctaacaccgg tttaattgcc
    28921 cgacgccctc tggattttcc gagggcgtat ttgtttctat gtccttcttg cccccgcccg
    28981 taaaaatatt ttcactttaa attcaatttg ataactacat ccattagcca cagcgcgatc
    29041 ctgtgcgaaa ttttgtgatc ttcctccaca ttacataaca tcatatgttg ttatattcat
    29101 catgcattgt catgttacct tttaaatgac tgcaaactct cccctacaac gtattggaca
    29161 agaaaaaggt atcgctatgg gaagccagga actccaacgc aagctcggat tttgggccgt
    29221 tcttgcaatc gccgtcggga caaccgtcgg ctccggtatt tttgtatctg tgggtgaagt
    29281 ggcaaaagca gcgggcacgc cgtggcttac ggtgctcgcg tttgtcattg gcgggttaat
    29341 tgtgatcccg caaatgtgcg tctatgcgga actatccacc gcttatccgg aaaatggcgc
    29401 agattatgtt tatctgaaaa atgccggaag ccgaccgctg gctttcctct ccggctgggc
    29461 cagcttctgg gccaacgatg cgccgtcatt gtcgattatg gcgctggcga ttgtcagcaa
    29521 tcttggcttt ttaacgccta tcgatccgtt gctcggtaaa tttatcgccg ccggattaat
    29581 tatcgccttt atgttgctac acctgcgctc cgttgaaggc ggcgcagcgt ttcagacgct
    29641 aattaccatc gccaaaatta tcccgttcac tatcgtcatt ggccttggga tcttctggtt
    29701 taaagcggag aattttgccg cccctaccac cactgcgatt ggcgcaacgg gcagctttat
    29761 ggcgctgctg gcggggatct ctgccaccag ttggtcgtat accggcatgg cctctatctg
    29821 ttatatgacc ggcgaaatta aaaaccccgg aaaaaccatg ccacgagcgc tgattggttc
    29881 ctgtctgctg gttctggtgc tctacaccct gctggcgctg gtgatttccg gcctgatgcc
    29941 cttcgacaaa ctcgccaatt ctgaaacgcc gatttccgac gccctgacct ggatccccgc
    30001 actcggcagc accgctggga tctttgttgc catcacggcg atgatcgtca ttcttggttc
    30061 gctttccagc tgcgtgatgt accagccgcg gctggaatac gcgatggcga aagacaacct
    30121 gttctttaaa tgcttcggcc atgtgcatcc gaaatacaac acgccggatg tctccatcat
    30181 cctgcaaggg gcgctgggga tcttcttcat cttcgtttcc gatctcacca gcctgctggg
    30241 ttatttcacc ctggtgatgt gtttcaaaaa taccctcacc ttcggctcca tcatctggtg
    30301 tcgtaaacgc gacgattaca aaccgctgtg gcgtactccg gctttcgggc tgatgaccac
    30361 cctcgccatt gcgtcaagcc tcattctggt cgcctcaacc tttgtctggg caccgattcc
    30421 cggccttatc tgcgccgtca tcgttattgc tactggtctg cctgcttacg ccttctgggc
    30481 gaagcgtagc cgccagctca acgctttgtc gtaatgttat ctggagaaaa taaaatgttg
    30541 gatattgata aaagcaccgt ggactttctg gtcaccgaaa atatggttca ggaagtggaa
    30601 aaagttctca gccatgacgt tccgctggtg cacaccatcg tggaagagat ggtgaagcgc
    30661 gacattgatc gtatttattt cgttgcctgc ggatcgccac tcaacgcggc gcaaacggcg
    30721 aaacatctgg cggatcgctt ttccgatctt caggtctacg ccatttccgg ctgggagttc
    30781 tgcgataaca ccccgtatcg cctcgacgat cgttgcgcgg tgattggcgt ttctgactac
    30841 ggtaaaacgg aagaggtaat caaagcgctg gagctgggcc gggcctgcgg cgcactcact
    30901 gcggcgttca ccaaacgcgc ggacagcccg attacctcgg cggcggaatt tagcattgat
    30961 tatcaggccg actgtatctg ggaaattcac ctgctgctct gctacagcgt ggtgctggag
    31021 atgatcaccc gcctcgcgcc gaacgcggaa atcggcaaga tcaaaaacga tctcaagcag
    31081 ttgccgaatg cgctcggtca actggtacgc acctgggaag aaaaaggccg ccagcttggt
    31141 gaactggcca gccagtggcc gatgatttat accgttgctg cgggtccgct gcgtccgctg
    31201 ggttacaaag aaggcattgt aacgctgatg gaatttacct ggacgcacgg ctgcgtgatt
    31261 gagagcggag agttccgcca tggcccgctg gagattgtcg aaccgggcgt tccgttcctg
    31321 ttcctgctcg gcaatgatga aagtcgccac accaccgaac gcgccattaa ctttgttaaa
    31381 cagcgtactg acaacgtgat cgtcatcgat tacgccgaaa tttcgcaagg gctgcacccg
    31441 tggctggcac cgttcctgat gttcgtgcca atggagtggc tctgctacta cctgtctatt
    31501 tacaaagatc acaacccgga tgaacgccgc tattacggtg gtctggtgga atattaatcc
    31561 ctctcccggc ccggcaacgg gccgaattta cgcaaggagt tacccgatga aaacaggtat
    31621 gtttacctgc ggccaccagc ggctgcctat tgaacacgca tttcgtgatg caagcgagct
    31681 gggttacgac ggcatcgaaa tttggggcgg tcgcccgcac gcgttcgcgc cggacttaaa
    31741 agcgggcggc atcaaacaaa tcaaggcgct ggcgcagacg tatcagatgc cgattatcgg
    31801 ctatacgcca gaaaccaacg gctatccgta taacatgatg ctgggcgatg aacatatgcg
    31861 tcgcgaaagc ctcgacatga tcaagctggc gatggatatg gcaaaagaga tgaacgcggg
    31921 ttatacgctg atttccgcgg cccacgcggg ctatctcacg ccacctaatg ttatctgggg
    31981 acggctggca gagaacctaa gcgaactgtg tgagtacgcg gaaaacatcg gcatggatct
    32041 aatcctcgaa ccgttaacgc cgtatgaatc gaacgtcgtg tgtaatgcta atgatgtgct
    32101 tcatgcgctg gcgctggtgc cttcgccgcg cctgttcagc atggtcgaca tttgcgcgcc
    32161 gtatgtccag gcggaaccgg tgatgagtta tttcgacaaa ctgggcgata aattacgtca
    32221 tctgcatatt gtcgacagcg acggggccag cgacacgcat tacattcctg gagaaggcaa
    32281 aatgccgctg agggaactga tgcgcgatat tatcgaccgg ggctatgagg gttactgtac
    32341 ggtggagctg gtgacgatgt atatgaacga gcccagactc tatgcccgcc aggcgctgga
    32401 acgctttcgc gcgctgctgc cggaggatga gagatgaaaa ccctggcgac aatcggcgat
    32461 aactgcgtcg atatctaccc gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat
    32521 gtggcggtgt actgcactcg ctacggcata cagccgggat gcattacctg ggtgggtgac
    32581 gatgactacg gcacaaagct gaagcaggat ctcgcccgca tgggcgtcga tatcagccat
    32641 gtccatacga aacacggcgt taccgcacaa actcaggtgg aactgcacga caatgatcgc
    32701 gtttttggcg actacaccga aggcgtgatg gccgactttg ccctgagtga agaggattac
    32761 gcctggctgg cgcagtatga cattgtgcac gcggcaatct ggggacatgc ggaagacgca
    32821 ttcccacagc tgcacgctgc gggcaaactt accgctttcg acttctccga caagtgggac
    32881 agctcgctct ggcagacact ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa
    32941 gaagacgaag cgctgcgtct gaagatgaaa gcgattgttg cccgtggcgc aggcgtagtg
    33001 attgtcacgc tgggtgaaaa cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag
    33061 gctcctgaac cggtgacggt tatcgacacc atgggtgccg gagattcgtt cattgccgga
    33121 ttcctttgcg gctggtctgc ggggatgaca ttaccgcagg cgatggcgca gggaacggcg
    33181 tgcgcggcga aaaccattca gtaccacggt gcctggtagg tataacgttg gcgtgagcat
    33241 cttcacgcca acgtgctgtt acttgccgga aaacgaccct ataatccgag taattcattc
    33301 tttatttcag ggtcgattat gtcagctacg gaccgctact ctcatcaact cctctacgct
    33361 accgtccgcc agcggttgct ggatgatatc gcgcaggggg tttaccaggc cgggcaacag
    33421 atccctaccg aaaacgagct ttgtacgcaa tataacgtca gccgcattac tattcgcaaa
    33481 gctatcagcg acttagtggc agacggcgta ctgatccgct ggcagggcaa aggcaccttt
    33541 gtacaaagcc agaaggtcga aaacgccctg ctgaccgtca gtggttttac cgattttggc
    33601 gtctcacaag gcaaggcgac gaaagagaaa gtgatcgagc aggaacgagt cagcgccgcg
    33661 ccgttttgcg aaaagctgaa catccctggc aacagcgaag tgttccatct ttgccgggtg
    33721 atgtatctcg ataaagagcc gctgtttatt gatagttcct ggatcccact gtcgcgttat
    33781 cctgactttg atgagattta cgttgaagga agctccactt atcagttatt tcaggagcgt
    33841 ttcgacacgc gagtggtcag cgacaaaaag accatcgata tctttgccgc cacccgcccg
    33901 caggcaaaat ggctgaaatg cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc
    33961 tttgatcaga atgacaaacc ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc
    34021 accttaacga ttgataataa aagacattaa ccgtaggccg gataagatgc gccagcatcg
    34081 catccggcga tgctggcgcg ttgaatttta catcccgtac gttcccctca ccctaaccct
    34141 ctccccaaag gggcgagggg accgttcagt acggaattta gcttgacggt gctatccagc
    34201 attaaatact ggcaatactc tcacgcaaaa tccgcagcac cgtctcttca ccgctgcgat
    34261 tcgggttaat gcgaatcgca caatgttctg attgtggatt cgcctggcga aacgttccgg
    34321 aaaggcgata aaagagcggc gggatttcat atttcgactc tgcacccacc gggtaaggca
    34381 aggcaccgcg cttttgcgcc tcttccagca ctctggcggc aatcggctga tgaaactcga
    34441 caatcaacac cttcgactgc gcattagcaa tcaccgcgct tttcacttcc ggcaccgcac
    34501 caccgttaag caaagccagc aaccgttcag atacccctgc ctgcaccgcg tgcatcactg
    34561 gcgcaaacac cagaccacgc aatacttcca gcgcctgtgc gccctggatc tggctaccgc
    34621 cggagtaaag cgtggcgcga atacggttga taacatcagc atcgccgacc actgcaccaa
    34681 caccctctgg cccaaatagc ttgaagcagg aaaatgtcga gacattcgcg ccgcattcac
    34741 agccgattcg cgccaccttc atcaccgcat agttgtcatc ggttaacgct ggaacacctg
    34801 ccgcgcgcaa cgttgccagc acatctgcca gcacgtagct gtcctgcggc tgctggcgcg
    34861 tatgctgcac cagcgccgca tccggttgtt gctcgtcgac gacctgcttc agtgccgaca
    34921 ggtcattgaa atcaacagta ataagcgtca gccccatctg ctcaataata acccgtgtcg
    34981 tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc cggtttgagc aaagccgcca
    35041 gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag cgccgcagcc tgtgcgtgaa
    35101 atgcatcagc cagcacctgc tccacccgtt gggtaatacg cggttgattc agacctggag
    35161 ttaaccctaa atcaccgcca gtaagaaact cgctgccggg gaaatggcga caaatgctat
    35221 ccaccagcgc aaactgcttt tgctgcgcct caataatcgt caggctttgc agaggaaacg
    35281 tcttcatagg tgccttcctt aagccggaac gaacaggcca agccagtaaa gaacattcag
    35341 cagaataccg gtgatcatta ctgccacgac cggtgccgcc attttctgta ccggacggcc
    35401 cagggattcg ttgaggaagt aaatggcaac cgcgatagag aatccggtat aacccgccat
    35461 cttaattgct gcgaaaatcg aaccgaccag cagcgccact tccatcagca tattcatggc
    35521 gttacggatg ttatccgacg cattacgcac cgacgggtag cgtcccagcc atttgccgat
    35581 cgaacgaagc agcaagactt ccgccgaaat aaccactgcg cctaataccg ccgcaaccat
    35641 cggatttggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa ctgcatacac
    35701 gccagtggct aacgcggtgg tggcaatcaa cggcacaaaa cccagtccgc gcataaattc
    35761 tgccagagcc gcctgattaa tcagcgtttg cgattgttcc ggcgtcacgc ctgcggaata
    35821 cgctttctcc agtgtgaaga tcgaaacttc gctaccagca aaaatcttca tactggcaac
    35881 ggcggcaatc aatgctccca cgatggcgat ataaggtaag tttttgataa tccgcgacgt
    35941 gcgctcttca aacaccgaaa gcccgctggc atcgatgtca ttttcatcac gatgacgcag
    36001 gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca ataaagattt cgatggattc
    36061 agggttaaga tgtgggaaat agcgcacgac taccacacgg gtcatcagaa caaccacggc
    36121 ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc gccaccagcg ggaacagtgc
    36181 aaaagcagaa accaccggcg agcttaattc cccgaggcta cctaatacat ccaccggcag
    36241 cgcggtcagc agctggttta ctggcaacag gcaagtaagg atcaacacgc cccagatagc
    36301 gccaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat cggtaggaag
    36361 aaagagtaac catgcgttga gcagcccggt tttcagggta aaagagatcc ccaccgaggc
    36421 cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacgat tcatatagcc
    36481 ttcaatcagt tgcggcagga tcgggcggat gccgtcatga aaaacagccg ccgagcgatg
    36541 cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa tatacagatc
    36601 catagtcgtg ccttttattt cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc
    36661 tcaacggaaa ggccaaacgc cactttccct tcagcgatca ttttggcgat atgctcgtct
    36721 ttcgctttaa tgcctggttt ggcaatggtg caacttttgt tatagccaat caccgcgatg
    36781 gcaatcgaca atgccgcgcc cgcaccggta ttacacgcac cgatgtagta atccagttgc
    36841 ccggatttca ctttcattgc cgcttccatg tcgttgtgaa tgaaaacttc aaaactgcca
    36901 ggagccgtcg cttcaatggt ttttttaatc tgctcacgct gtaagcctgc aacgccaatc
    36961 tttttcatga gtctgtcctt attggaaaaa ttgagaggga ttttcacgta acatcacatc
    37021 cacatcggcc tgactgaatc ctgactggcg caattgcgga ataaaggtgg tcaataaata
    37081 gtcatagcca taaccaccat tggcttttaa atgggagcgg cgcgtaatat ccatcgacag
    37141 catgacgcgg ttcagcaacc cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc
    37201 gtccgggtag taactgttct taccaatggt gtcgaactgc acgtacgcgc cgagatcgat
    37261 catcttcaaa atgttgtcga gattgtcttt cagatcgcag tgaccaacgg tgacgcgcga
    37321 aagatcaact ccgtgggctt gcaacagagc cagttgttcc agccccatgg tgctgaacga
    37381 agtatgcgtg gagatcgggc gtccggtctg gttatgcgcc agcgcagcgg caataaacac
    37441 cttctcttcc agcggcgtaa tcttcccttc gctagagccg atctccgcga taatcccggc
    37501 tttcaggtcg gtgccatcga taccctgttc aatttcatcg accatctcct gcgccagttc
    37561 ctgcacgctg cgggtcgcca catgctccgg gaaaaaagcg tcctggtaat aaccggtgca
    37621 ggccaccacg ttgatccccg tctcgcgcat tacatcgagc ataaattgcg cattgcgccc
    37681 catgtaacgg ttggtcatct caatcacatt acgcacgccc cgggtcatca ggtcgttcat
    37741 ctcctggcaa atgaacgcat actgatcaag gcggcagtcc acgttgtttt taaagccgga
    37801 gagatcaata tgcagatgct catgggccag ggtgtaaccc gtcggatcaa tactcatagg
    37861 gtgtctcctg caaaccggag ggaggaaaga aaagagcgac cattttgtgg cggtggcgca
    37921 cggaaaaatt cacacacggt agcccccaca tcagaaagcg tggtgcgtac accgagttgc
    37981 gtgtggacca gcccttgctg ataaaccagc actggcacca cttcacgggt atggtggctg
    38041 tgaccaatgg tcggatcgtt accgtgatcc gccatcacga ccaggcaatc atctggctgc
    38101 atcgcctcaa caagccgggc aaggttacgg tcaacgacct gcaaacgttc ggcataacgt
    38161 gcgacgtctt ctgcatgacc agcgaggtcg gtttcctgga tattgatgca aataaacgcc
    38221 gtcggatgcg tgttaaattc gtcgagggtg atatccataa tccgctggct atccaccagg
    38281 ttttgccagc tcacgccata aggattgctg acgatatctg ccaccttacc taccagcacg
    38341 gttggcacgc ccgcctcata cagtttttgc ggcacctgca ctttttcatc aacaccgtag
    38401 cccatatgca cgacctggaa gccgttgtca taagcgccag aacgcggcgc attaatacca
    38461 ataaagcgcc cttctttgct ttctgcggca tcgagaatac gctgactgtc ggttaacaag
    38521 ccgccaaatg taatgacccg accgacctgt acctgctcac gcacgatacg accaattttg
    38581 atcgcgtcgt caaaagagat cacagagaga ttggcggtaa tgttatagac ctggcctaaa
    38641 tccgcctcga gattatcgcc aatcgcaacc gcctgattga cccacagaaa ttgcagatca
    38701 tcgccacggc gctccacctg ccagccagcg gaaactaatg cctgctcaac acggccaatc
    38761 acatcgcgaa agggcatccg cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc
    38821 cccataaagg tatcgccacc ttcatgttgc agctctgcca ccccccaggt tgcggaatct
    38881 gacggttgca tatcacctgg tgcataaccc aatgcgttga ttagccccag cttctccagc
    38941 gctggtagct gcaaatgcgg caactggctc aggatgtgac cacaggtatt cgccccggca
    39001 tcttgcgggc gcaccagcgt gacatctttc attgccccta cgccaaagct atcaatcact
    39061 aacaccacaa atcgcgccat tacgccccca gagagtttcc aagactgtca taacgaccga
    39121 cgattttcgg ttcgccacgg tgaataccgg acaccagcac cacatcgcta cgagtgacaa
    39181 aaatctgcgt gcgaaaacag agcactactg cactgcttac cggaaactcg cctgccagcg
    39241 gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca gtaatctttt
    39301 gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc acgacggtag taaccgccgc
    39361 cgtagcagta gctgtcgcca cggaaatgat gggagatttc acttaaccaa agcatcgcta
    39421 tatattcagg ctgatcgccc tgctggtttg ccggaatagt gcccgtcagc gcatgaccgg
    39481 gttcggcatg agtcacaccg tattccgcca gcatcggcag cgaagtgcag ctggtcgcag
    39541 aaggcgcgtt cagttgctca atcgcaatac cagatttcgc cagttgatcc cgtgcttgta
    39601 ccagcgtgtg aagattcggt gtcggcaaaa ctttcccggc agcctcatcc caaagcaggc
    39661 aagggaaatg ggtaagtccg gctaaatgca gacccggcag tttcttaatt tcagcaacca
    39721 cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc cggataaaga aaatcatcgt
    39781 cgctataaac tttgagcagc acaaactgca cacgtccggc cttcaccgct gccgcagaaa
    39841 cttcccgcgc tttgtcgaga gtaaacacgg tgatgacgtc ggtgccctgt tcaacggcgt
    39901 cagcaacctg atgacaaggg atttgtacca gatgcccctg atgcgccacc gacaaaccag
    39961 cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac aatgccgctg taacctaatg
    40021 ccagcaattt ttccgccagc catgggttac ggccaaactg cttggtcatc agatacagtt
    40081 caatgccgta aagccgcgcc gtctcaatca gtcgcttacc gttttccagt acctgatcca
    40141 cgtcgatcac ccagctgtcc ggggcgatct tgccctgctg ccacaaactt agtgcggcgg
    40201 aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa catcttgttg cctcgtttct
    40261 gttccttgaa ttaaatattc atttttttga atatttaggt tgtgcgacgt ggatttgtcg
    40321 ggagcttcaa acctcttcgt tagcagccat ccataatcca taaaggttgg ccagcaaata
    40381 gccttcttca cacgggttaa cttccagtgc gaattccttc aacaacacct gatgcaattg
    40441 cacaacggct tgccagtggc tggattgcgc cagctctgcc agcaattcgt tatccagcgg
    40501 ctctatttct tcaccacggc gactgcgcat cagtgcactc gccatatgtg tcatcgccat
    40561 cgttccttgc tcactgcgca ccggcagatg gcactctgtt tccagtacgt tgacgacctg
    40621 catcatgcct ttgcagatgt ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct
    40681 ggtttccata tcagatgcct ttcgtttaat aactcggttc ctgttcccca ctcactaccc
    40741 gttgttgatg ggcgagcagg gcgtgtttat ctacaacggc acgcagcagt tgttgcattg
    40801 ggtaatcatc gactcgcgtc agaaccacgg cttcggtggc ctgtaaaaat cgcggatcgt
    40861 ctgtgagcgg cgtcgcctct aatcccagca tggtcagttc gttttccgcc accacgttcc
    40921 agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa actctcgtga taagagagat
    40981 cgacacgttc cacatcacta tcgccaaaaa agacatcggt catgattttc tgatccgccg
    41041 aacggttatc cagccccacg cgcttcacgt ttgcggactc gcctttacgg caaatcaact
    41101 ggtgctcgcc aacgtaggtg tgcggcccca actccagcgc gaggcaaaac cctttttgcg
    41161 tgagataact ttccgccgcc agtcgggaaa ccaccgccat gtcatacacg ccgttaagta
    41221 aacactccac gcgaatatcc gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa
    41281 actgggcttt caatccgctc gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg
    41341 cacataccac gttgttgata tccacatgac tcagcaatgc tttgttatcc atctcgacca
    41401 gataactgcc attgcgccca cggcgttcaa tccgtatcgc tccgcttgat tccagcgttt
    41461 tcaacgcggc ctgcgtcagg ccaacggatg aacggcattc agttgccagc tcatctatgg
    41521 ttttcaacct attaccgcat ttttcaccca acaaataacg ggccagcgtc gtgataacga
    41581 cgccttcttt tttgataaac gttcgacgca taatatattt tcaataaaat gaatgtttat
    41641 atcttcatta aaatgaagat aagtggcaac tgtgagatgt ggaaacggcg aggcgcttca
    41701 cattttttcg gattattgca aatgaggata aaaaaaccgg gtttccccgg tttcagagtg
    41761 atgacgaagt gatttattcg acaatgcgag caaagggtaa ttcccactgg cgcttgcctg
    41821 ctcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgagc gttttgatat
    41881 tacggcttcg ccacaaaacc aatcgcttcg tacaccgctt tcagcgtacg ggaagcgtgt
    41941 acactggctt tttctgcgcc gtctttcatc acctgttgca ggaaggcttc atcgttgcgg
    42001 aaacggtgat agcgttcctg caattcagtc agcataccgg aaacggcatc agccacttca
    42061 cctttcagat gaccatacat cttgccttcg aactgttttt ccagttctgg aatgctctgg
    42121 cccgttaccg cagaaaggat atccagcagg ttggaaacgc ccgctttgtt ctgcacatcg
    42181 tagcgaacta ccggcggctc gtcggagtca gtgaccgcgc gtttgatttt cttcactacc
    42241 gatttcggat cttccagcag gccgataacg ttattgcggt tatcgtcaga cttggacatc
    42301 ttcttggtcg gctccagcag cgacattacg cgcgcgccag atttcggaat aaacggctcc
    42361 ggcaccttaa agatctcgcc atacagcgcg ttgaaacgct gggcgatatc gcgactcagt
    42421 tccaggtgct gtttctggtc ttcacccacc ggtaccagat tagtttgata cagcaggatg
    42481 tccgcagcca tcagcaccgg atagtcaaac agaccagcgt tgatgttctc ggcataacgc
    42541 gcagatttat ctttaaactg cgtcatgcgg ctcagttcgc cgaagtaggt atagcagttc
    42601 agtgcccagc ctaactgcgc atgttctggt acgtgggact gaacaaaaat ggtgcttttc
    42661 tcaggatcga taccacaagc cagatacaag gccagcgtat ccaacgtcgc tttacgcagc
    42721 ttctgtgcat cctggcgcac ggtgatcgcg tgttggtcaa cgatacagta aatgcaatgg
    42781 tagtcatcct gcatgtttac ccactgacgc agcgcaccca tgtagttacc aatggtcaat
    42841 tcacctgagg gctgtgcgcc actaaaaacg atgggcttag tcatttttcg attcctgatt
    42901 ttcgctatgc ggaagcccta atgcgggcag aaggtcattt atagactgat aaattacatc
    42961 aggctggctg agatcgatag cctcgccgta gttatatccg taggttaagc caactgatgg
    43021 gcaacctgcc gcttttgccg cctgaatatc attgcgtgag tcgccgacaa acagcatctg
    43081 ttgtggggca attcccatcc gctcagccac cagtaacagc gggtccggat gcggtttttt
    43141 gttttgcaca tcatcgccgc cgataaccac gctaaagtat ttggcaatat ctaaggcttc
    43201 gagcagcggc gcgacgaacg gcgtcggttt gttggtgacc aggcctagcg gcaggccttt
    43261 agcctgcaac gcgcccaacg tatcggcaac gtgcgggaac aaaaacgtcc cctcttcggc
    43321 aacctcgcca tagtagcgat cgaacagttt acgcagaata cgtacctgtt cttctgccgg
    43381 aatgtcgtca tcaacgggcg gtttacccat tgttttacgc tgagtcgcac gttcctgacg
    43441 cgcccaggtc aatgcgcgct ccatcagaac atctgcgccg ttaccaatcc aggtaataac
    43501 gcgttcttca cctgcgacgg gcaactccag cgcatacagc gccatatcta ccgcagcagc
    43561 aagaccggga gcactgtcga ccagcgtacc atcaagatca aaagcgacgc cgcgaatatc
    43621 ttcaaactta ttcatgactt acctttgcca gttcactgcg catttcatca atgacttttt
    43681 tgtagtctgg ctggtcgaag attgccgaac cggcgacgaa catatccgcg cccgccgcag
    43741 cgatttcgcc aatgttgttc accttcacgc caccgtctac ttccagacga atgtcaaagc
    43801 cagactcgtc gatacgacga cgtacttcgc gcagtttatc cagtgtttga ggaatgaacg
    43861 actgaccgcc gaaaccagga ttgacggaca tcagcaggat cacatccagc ttatccatca
    43921 cgtaatccag atagctcagc ggtgtcgccg ggttaaatac cagtcccgct ttacagccat
    43981 tttctttaat cagttgcagc gtgcggtcaa catgctcgga ggcttctgga tgaaaggtaa
    44041 tgatgctggc accagcggca gcgaaatcag gcacaatgcg atcgacaggt ttcaccatca
    44101 ggtgtacgtc gataggggcg gtaatgccgt agttacgcaa ggatttcagc accattggcc
    44161 caatcgtcag attgggaaca tagtggttat ccatgacgtc aaaatgcacg acatcagcgc
    44221 cagctgccag ggcttttgcg gtatcttcac ccaggcgggc aaaatcagcc gacagaattg
    44281 agggggcaat caaatactgt ttcatccgct tctccttgag aattattttt tcgcgggtga
    44341 aacgactcct ggtttgtaca aagccagcag ttcgtccacc tttttacgtg tgccgccgtt
    44401 gctgcttata ctgcgtcgaa ctttgacgac atgcaatttt gcgcgctgat accactcacg
    44461 cgttaacatc gtatcgtgat tggagatcag cactggaata tggcgctcaa ccagaccttc
    44521 ggcgatctcc gccagatgcg cttgttgttc aagcgtaaaa ctgtttgtgt gatacgccgt
    44581 aaagttggcg gtcgcagaca gcggtgcata aggcggatcg caatagacga cggatgcatc
    44641 atctgcgcgc gccatgctat cggcgtaaga ctcacaatag aaaaaggcat tctgcgcttt
    44701 ttcagcgaag tgatacaact ctgcttccgg gaaatagggt tttttgtagc ggccaaacgg
    44761 cacgttaaac tcaccgcgca gattgtaacg acacaggccg ttgtaaccgt agcggttcaa
    44821 atataaaaac agtaccgccc gacggaacgg atcctggctt ttgttgaact cttcgcggaa
    44881 ctgatagtag acctcggcgc aatttgtttc gggaacaaac agctcgcgtg cggcctgtac
    44941 gtactcatca gtacgcatct tcacaatgtt atagagactg atcaggtcgc tattgatatc
    45001 ggcaaggata taacgagaaa agtcggtgtt gagaaacacc gacccggcac ctacaaaagg
    45061 ctcaaccaga cattcgccct tgggcaaatg ccgtttaata tcatcaagca ggggatactt
    45121 gccccctgcc cacttcaaaa aagcgcgatt tttcttcatg ctgactaact aattacacct
    45181 tctccggctg tggagaaagc tccgacagca tccagcgctt caggcaactc ccgcaacgat
    45241 aacccattgc gggagataac cttgattact tcagatcggc ctgtacctga cgcagcggtt
    45301 tcgcccacgg gtttttggcc tgaacatctg ctggcaatgt agataccgct tttttcgcct
    45361 cttctttcga agcgtacacg ccagaaacca ggacatacca cggctgacca ttacgcgtcg
    45421 tttcatagac aacgtagttt ttcagattct ctttcttcgc ccaaccgttc aggttgtcgt
    45481 agttagagga actgctcagc tgcagagtgt aatggctgga cggtgccgat ttcaacgaac
    45541 caacattacc tgcggtcttc cctccagcgg ctggtgttgc cgtggtttgc gccggggatg
    45601 ccgtctgtac tggagccgta gtcgccgtct cttttggtgc tggcgcagaa gtcgcagccg
    45661 gtgccttcgt gctcgccaca ggagcagctg gttcagtacg cttcggcgtc tgtgctaccg
    45721 gcttcggttc cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac
    45781 gagctggacg cgtagtggcc ggacgttcag cagtttgcgt tttcgccgtg tcacgcgatg
    45841 cattgccatt gcgtactggc gcgacagttg ctggttcagt cggcaatgta gaattgaccg
    45901 ccacattgtt caactgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct
    45961 gcacttcaac acgttgttga ccatccgttg ccgccggggt ttgcccttga gtcggcgtag
    46021 aagagatcgg tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg
    46081 ctggctgcac cccattcgcc tgatcggtcg cattgcctgc aagatcaata ctcttctcgc
    46141 cagacgcggt ttgatcgctg gaagaggtcg agggggcttt tagcgcagaa ccgataccga
    46201 tgatcaacag cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg
    46261 gtttactggc tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg
    46321 tttcatcttc ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt
    46381 gcgcacgagt cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct
    46441 gcggttcgcc acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac
    46501 gacgatcgct gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc
    46561 ccactaaaag gttaatgctt accacattgc aattaacctg aagctaatag accgcttgat
    46621 aagcggcctg acctttcttg ttgttacgct gattgactat cggcaatggc gttaagaaca
    46681 agctcgtgcg aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag
    46741 cgtatctctc ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg
    46801 gacatttcgc gcggcccatt gaccggtaac ccagcccgct tgagcagggt tataatacgc
    46861 tgcgtttcgg cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata
    46921 cccgcagcga ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca
    46981 tgaccaaagg tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg
    47041 gcgacaactt ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg
    47101 tccagacgca acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga
    47161 ataatgccgt atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt
    47221 ttcagacagt cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt
    47281 ttaccgaggg gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt
    47341 aacgtcgtcg ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg
    47401 gtcagatcgc ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc
    47461 ttttgtaaca acgccgtaaa gacggtatcg agtacagcca ggcttttata ctgctcgccg
    47521 tcagggagga taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta
    47581 tcgagataca gaggagccag ggtttcgttg gtgaccaaca tgacctgctc gcccgatttc
    47641 agcggtaaga atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta
    47701 cgttccccga gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac
    47761 ccgcagacga gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt
    47821 ttgcaaccac tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat
    47881 acagcggatt gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt
    47941 gcagcaacgg gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt
    48001 caagataaac gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag
    48061 agccgccgcc agtagccagc acaataccct gtttctcggt caactcattg atgacctttt
    48121 cttcgcgatc gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc
    48181 cggttcgttt ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta
    48241 actggcgccc aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt
    48301 tctctgccat tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca
    48361 gagagcgccg caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa
    48421 ttatctcaat actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct
    48481 ggtcgctgca agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat
    48541 tagcgtaaaa aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg
    48601 cagcgaagag caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc
    48661 aaatagatga aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg
    48721 gaaaccagtc gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca
    48781 tgacgaaata attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc
    48841 gatttatttt tacgggtaaa aatgccgccc agcgccaacg tttctccgct tttgacctcg
    48901 acctgcgttt cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc
    48961 acctgccccg gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac
    49021 accgtgggcg tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca
    49081 ctttccccgc tggaaacctg atatggaatt tcgctcccct gtttaatgct ggcaggctga
    49141 agatgtgagg ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc
    49201 gcagaaagct caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc
    49261 gttgtcgccg tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca
    49321 gcgtgttgcg catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta
    49381 atggtgacaa tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc
    49441 cactgttcaa gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt
    49501 ttatcgacgg tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct
    49561 cccgcgtcgg cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc
    49621 cgcgcctgct ccgcctcctg gcgggcgata ttgttattct gccaggcaat ggaatgcact
    49681 gagagaatgt tgtcttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt
    49741 gcctgcttcc agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac
    49801 acgaccaggt tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg
    49861 tcatccacca tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc
    49921 aacagtagtg cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg
    49981 tggcggttcg cagtttgtcc cggttcccag cgttagtgcg tctggcgtca gctgtaaaat
    50041 tgtccagccg ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc
    50101 ttttattaca ccgatgatgt gctcgcctcg ccctaccatc ccctgatagc gccactggct
    50161 aagttcgcta atccggcata gatcttccgg cggtttaaaa gggtcacgca taccggttaa
    50221 aaggcacaat gcaatacctg ccaacaacca gcgtttaacc ctcatgcggt gtctccagtt
    50281 gtagcgtgaa caaaagatca tcaccttcca cgcttaacga aaaacggctc acgctgacgt
    50341 tgcgctctgc cagccgtgta aatgccgacg gcactgcttc ccacagcgtt ttcaacgcca
    50401 actcacctcc ctgcgcggat ggatgccagg aaaccagttg cgcgccgggc aactgaaaat
    50461 ccagtggcga aaagggcagc gttttttctt cgctgaaggg agtggtgtca accaggcgat
    50521 acagtgtggc ccactgctgg tgatgacttg cccgtagtcg aattaatgcg tcgcgctctt
    50581 catggtgtgt cgatgacaga aaaatgagcg taactaacat cagcaaccag aatgcccagc
    50641 aaaactggcg gaggcggggt gatgtggcga accaccagtc aaagaacatg ttcatcgcta
    50701 accttccttg ttaactgata ctcaaattgc cagcgtccct gcgcatcctg ctgcgtggct
    50761 ccccgctgat tgagatgaaa agacgcatcc tggcggagtg acgtttctag tgcgtttaac
    50821 gcggtaatgc ttgttgtcag ccccttgatc tccagcgttc cctgctgcca gcttatcgtt
    50881 gtcagccagg cgtgctcggg taaaagcgcc gccagtgctt ccagcgcaga ttgccagtcg
    50941 cgggtaaatt gtcgctggcg ctgccgttgc gaacgctgtt cgcgtaactg ctgttgctcc
    51001 agcaaacgtg gcttcgttat ctgtaagctg cgggcgagtt gttgttccgc ctgaagcaaa
    51061 actgcgtcta tgcgcgcttc ggcgctgcct gtcagacgca gtattagcgt tatcccgacg
    51121 gccagcagca gaggcgcaac gaacatcagc aaccagaaac gcagaaaagc ggtccggcgt
    51181 tgctgtcgcc agggcaaaaa attaattggc gggttcatca gtacgccttc ccaagcgcca
    51241 gccccagcgc gatggcaaag tctccaccag gcggcggtag cggcggctga cgaacagaaa
    51301 cggcctccca ggggtcaaat ccgccttcgc cacatatcgc gacgctgtcg ggatcaacgg
    51361 ataacgctgc cgccagctcc ttcgcgctag tcatccccac cgccagtttg cgcccccagc
    51421 tatagcgcgt cgcccacagc cactgttcgt tatcacgcca ggccagacat tgctgatgag
    51481 aaggtaaaaa aggcaggaat cgctgtaatg cactggcatc cggggtgatc gcactaacat
    51541 gaacacgcaa cctttctgcc agagtaagca gcgttgccag ctctttgctt tgcgcggcag
    51601 tcacgttata ggcgggactg agtgagtctt cgctgtaatc gaaacgcagg gagtccggat
    51661 ccatatccag ctcgcgggcc atcgtccctg acagccaggc cgtttgctcc cgctcaccaa
    51721 gggacatcga cgggcgcgga aatgaccgct gtaatgtgcg actggcggga aacgccaaca
    51781 taatgtgatg acgctgcggc agttcgcgac tccacggtaa caacgtttta gccagctgct
    51841 gcgcatcaac aatccgccca tctttgataa tgtcgttctc cagcggcaac cgccaccagc
    51901 gttgcaaaaa gcattctttt gctccccgca cgatcgcaac cgctaccgct tcttgctgtt
    51961 gtaaatgcaa accaatttgc cagatcttaa atgccattgt gatgctctcc ttatcacccg
    52021 tcactctgac gggtatatca atgcgtctgg cttgccttta tactaccgcg cgtttgttta
    52081 taaactgccc aaatgaaact aaatgggaaa tttccagtga agttcgtaaa gtattttttg
    52141 atccttgcag tctgttgcat tctgctggga gcaggctcga tttatggcct ataccgctac
    52201 atcgagccac aactgccgga tgtggcgaca ttaaaagatg ttcgcctgca aattccgatg
    52261 cagatttaca gcgccgatgg cgagctgatt gctcaatacg gtgagaaacg tcgtattccg
    52321 gttacgttgg atcaaatccc accggagatg gtgaaagcct ttatcgcgac ggaagacagc
    52381 cgcttctacg agcatcacgg cgttgacccg gtggggatct tccgtgcggc aagtgtggcg
    52441 ctgttctccg gccacgcgtc acaaggggcg agtaccatta cccagcagct ggcgagaaat
    52501 ttcttcctca gtccagaacg cacgctgatg cgtaagatta aggaagtctt cctcgcgatt
    52561 cgcattgaac agttgctgac gaaagacgag atcctcgagc tttatctgaa caagatttac
    52621 ctcggttacc gcgcctatgg tgtcggtgct gcggcacaag tctatttcgg taaaacggtc
    52681 gaccaactga cgctgaacga aatggctgtg atcgccgggc tgccgaaagc gccttccacc
    52741 tttaatccgc tctactcaat ggatcgtgcc gtcgcgcggc gtaacgtcgt actgtcgcgg
    52801 atgctggatg aagggtatat cacccaacaa cagttcgatc agacacgcac tgaggcgatt
    52861 aacgctaact atcacgcgcc ggagattgct ttctctgcgc cgtacctgag cgaaatggtg
    52921 cgccaggaga tgtataaccg ttatggcgaa agtgcctatg aagacggtta tcgcatttac
    52981 accaccatca cccgcaaagt gcagcaggcc gcacagcagg cggtacgtaa taacgtgctg
    53041 gactacgaca tgcgccacgg ctatcgcggc ccggcaaatg tgctgtggaa agtgggcgag
    53101 tcggcgtggg ataacaacaa gattaccgat acgctgaagg cgctgccaac ctatggtccg
    53161 ctgctgcctg ccgcagtcac cagcgccaat cctcagcaag cgacggcgat gctggcggac
    53221 gggtcgaccg tcgcattgag tatggaaggc gttcgctggg cgcgtcctta ccgttcggat
    53281 actcagcaag gaccgacgcc gcgtaaagtg accgatgttc tgcaaacggg tcagcaaatc
    53341 tgggttcgtc aggttggcga tgcatggtgg ctggcacaag tgccggaagt gaactcggcg
    53401 ctggtgtcga tcaatccgca aaacggtgcc gttatggcgc tggtcggtgg ctttgatttc
    53461 aatcagagca agtttaaccg cgccacccag gcactgcgtc aggtgggttc caacatcaaa
    53521 ccgttcctct acaccgcggc gatggataaa ggtctgacgc tggcaagtat gttgaacgat
    53581 gtgccaattt ctcgctggga tgcaggtgcc ggttctgact ggcagccgaa gaactcacca
    53641 ccgcagtatg ctggtccaat tcgcttacgt caggggctgg gtcagtcgaa aaacgtggtg
    53701 atggtacgcg caatgcgggc gatgggcgtc gactacgctg cagaatatct gcaacgcttc
    53761 ggcttcccgg cgcaaaacat tgtccacacc gaatcgctgg cgctgggttc agcgtccttc
    53821 accccaatgc aggtggcgcg cggctacgcg gtcatggcga acggtggctt cctggtggac
    53881 ccgtggttta tcagcaaaat tgaaaacgat cagggcggcg tgattttcga agcgaaaccg
    53941 aaagtagcct gcccggaatg cgatattccg gtgatttacg gtgatacgca gaaatcgaac
    54001 gtgctggaaa ataacgatgt tgaagatgtc gctatctccc gcgagcagca gaatgtttct
    54061 gtaccaatgc cgcagctgga gcaggcaaat caggcgttag tggcgaagac tggcgcgcag
    54121 gagtacgcac cgcacgtcat caacactccg ctggcattcc tgattaagag tgctttgaac
    54181 accaatatct ttggtgaacc aggctggcag ggtaccggct ggcgtgcagg tcgtgatttg
    54241 cagcgtcgcg atatcggcgg gaaaaccggt accactaaca gttcgaaaga tgcgtggttc
    54301 tcgggttacg gtccgggcgt tgtgacctcg gtctggattg gctttgatga tcaccgtcgt
    54361 aatctcggtc atacaacggc ttccggagcg attaaagatc agatctcagg ttacgaaggc
    54421 ggtgccaaga gtgcccagcc tgcatgggac gcttatatga aagccgttct tgaaggtgtg
    54481 ccggagcagc cgctgacgcc gcctccgggt attgtgacgg tgaatatcga tcgcagcacc
    54541 gggcagttag ctaatggtgg caacagccgc gaagagtatt tcatcgaagg tacgcagccg
    54601 acacaacagg cggtgcacga ggtgggaacg accattatcg ataatggtga ggcacaggaa
    54661 ttgttctgat taaaaaggcg cttcggcgcc ttttcagttg gctgacaaag tgcatttgtt
    54721 tatgccggat gcgggtgaac gcgttatccg gctaacaaaa tcgtgaaaaa atcaatatat
    54781 tgcaggaaac ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac
    54841 tcgcccctgc cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc
    54901 gttgaagtca gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg
    54961 tagcggctct ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat
    55021 attcattttg ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc
    55081 gttcgcacca aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc
    55141 acccggatca attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta
    55201 ttcgcggatc aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt
    55261 ggttggacgc attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct
    55321 ctcgacggta aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg
    55381 taatgatttg ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg
    55441 acacgctttc ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc
    55501 gtcctcaaat caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc
    55561 cgccttcaga gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt
    55621 taaactccac gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga
    55681 ccacgcctga cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc
    55741 tgctttgctg gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg
    55801 tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga
    55861 acgtagcgcc gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc
    55921 aacgggagcc agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat
    55981 gatgctgaca cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg
    56041 ttactacctc gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga
    56101 tgagaatacc gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg
    56161 tcatacgctg caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc
    56221 cacgcaggcg tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa
    56281 agaaacgcat gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat
    56341 cgtcgcctcc ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg
    56401 gctggcaggc ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc
    56461 ggcaaaatcc tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt
    56521 gtttggcgaa aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt
    56581 ctatcccgca cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga
    56641 tatctatctc gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt
    56701 aaaaaacttt ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct
    56761 ggtgctgttt atgctgttat tctgggtccc gctggatatg ccgctgaaat tcactctctc
    56821 atggatgaaa ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg
    56881 cgtgcgggta ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc
    56941 cggaacctgg agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat
    57001 ctggaatgac gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc
    57061 attgactgaa gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag
    57121 tgcctcatta cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg attttggcga
    57181 cattgtactg aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa
    57241 tgcgctggtc aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc
    57301 cgggaagctc gatggcgtga atgtgttatt acgcccggtg agtgcagaat cgctggataa
    57361 cctggtggca acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc
    57421 actaaacagt ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt
    57481 tgatcagccc tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc
    57541 tttccagaaa ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac
    57601 aaaaatcttc actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg
    57661 ttccggactg tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc
    57721 catttacaat ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga
    57781 gattcaggcc tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct
    57841 tatcgaataa cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc
    57901 tggagatttc cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg
    57961 atatggacgg cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc
    58021 ctgaaacatg gggcgccaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc
    58081 agcaatatca cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc
    58141 aactgggtct ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg
    58201 aagataccat tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca
    58261 ccaatgcgca tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc
    58321 ttgatttatt actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc
    58381 atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg
    58441 aagcgattct cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc
    58501 ctgattccgg gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc
    58561 gcctgatccc ctcgctaatg tgaaggagac gccatgaaag agaaacctgc tgttgaggtt
    58621 cgactggata aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa
    58681 atgattgaag gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc
    58741 gagctgaatg ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag
    58801 gcgattactg aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg
    58861 gaaagtgtag agaaacgcga aaaaatggcg atggcacgta aacttaatgc cttaaccatg
    58921 ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac
    58981 ggcgacagtg aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta
    59041 catcgctatc tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc
    59101 ctgcaacaga tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa
    59161 ctgctggttg cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta
    59221 cagctgcaag gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa
    59281 atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg
    59341 gtcggcaatg gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc
    59401 gtggttggtc tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct
    59461 gaacagctgc cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca
    59521 ggcggtatgt tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac
    59581 ctggcgacgc taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa
    59641 gtgttgtggc gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag
    59701 ttcaaatgca cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa
    59761 gaagttgata gcatcctgac ggaagatggc gaaattgaca tgcattgtga ttactgcggt
    59821 aaccactatc tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca
    59881 gatccgcaag ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgacg
    59941 taatggcctg atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat tattgttggt
    60001 cagatacagc gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt
    60061 tcaccatcta ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc
    60121 gcagctgtga gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg
    60181 tcccattctt ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca
    60241 aacaggatcc gctccgcctg accatcacta ttcagatctt gctcgaccag gacgcaggcg
    60301 tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc
    60361 gcatccggtt taacagaacc aggggcaatt aacacatttt cggctaatac tttttccgat
    60421 acctgttcct gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc
    60481 gggtctttca taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga
    60541 ccgctctgtt cgagcatgta gatagttacc tggtctggcg tgtttttgcc gctctgataa
    60601 cgcgccatat gactgttcac gctaatacgc atactatcca gcaccggcga attaagaagc
    60661 accagtatca ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg
    60721 ggattttgcc cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac
    60781 accagcaccg ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc
    60841 agccataacg cccaggcggc gacgaacaca tacagcggag caaccagcaa agcggtttta
    60901 atcaggcaac gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata
    60961 gccattaaga tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg
    61021 cgagaaatcg cgctcaggcc cgtaaacggc agagtgatga taaacatcag ggttagcaat
    61081 gatactaacg gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat agagtcgatt
    61141 aaacgtgact gtgtgcgcgc gagaatcacc gccagtgcgg taaccagacc taatgtaaga
    61201 tacataaacc agtcggttgc aaaaaagagc gtattgaaaa acttgatgcc aacaagttta
    61261 aacaactcac cccacagtaa aagcaccacc cacgtcaggc cattggcgag aaaaatcacc
    61321 agtaatataa gtacgttatg ccatactgac ttgtaaaagt agctatagcg gctactgcca
    61381 ttgcggatac gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga
    61441 tagcagccaa aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc
    61501 cacttcagcc aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa
    61561 aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcaacggtc
    61621 gcaggcacgc cataaacaat ccaactgtga ttttttccgg caagccaggc gatcaacagg
    61681 tagcaaacca gcccctgaag taatccggta gcaatcatcc cccagcgtgt tgccggtgaa
    61741 agttcaacgt tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg
    61801 ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat
    61861 actttaccga ttgaatttgc atcaatttca ttaaggaatg cgattccact cacaatattc
    61921 ccgccatata aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt
    61981 ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt
    62041 tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact
    62101 attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc
    62161 cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct
    62221 acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt
    62281 taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag
    62341 ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca
    62401 aaggtaagaa cgacaacaaa cctctctctc cggaaacctg gcagcatctg aaaggcctgg
    62461 tgaccaggca gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc
    62521 cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca
    62581 aaaacatgtt tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg
    62641 ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga acagggtctg aactccgaaa
    62701 acttcgtggc gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg
    62761 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg
    62821 cttctatgca ctgctccgcc aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc
    62881 tttccggcac cggtaaaacc accctttcca ccgacccgaa acgtcgcctg attggtgatg
    62941 acgagcacgg ctgggacgat gacggcgtgt ttaacttcga aggcggctgc tacgcaaaaa
    63001 ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgcgatgcgc
    63061 tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa
    63121 ccgagaacac ccgcgtttct tatccgatct atcacatcga taacattgtt aagccggttt
    63181 ccaaagcggg ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc
    63241 cgccggtttc tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg
    63301 ccaaactggc cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct
    63361 tcggcgcggc gttcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta
    63421 tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac
    63481 gtatctcgat taaagatacc cgcgccatta tcgacgccat cctcaacggt tcgctggata
    63541 atgcagaaac cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg
    63601 tagacacgaa gattctcgat ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa
    63661 aagccgaaac cctggcgaaa ctgtttatcg acaacttcga taaatacacc gacacccctg
    63721 cgggtgccgc gctggtagcg gctggtccga aactgtaatg atttgaagct ggagaatatc
    63781 tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct
    63841 tcttttgtcg tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg aatggaaagc
    63901 ccgccccgct cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc
    63961 acaattgcca gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac
    64021 ggctggaaca ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg
    64081 aaccaggcgc gattcggctc cgttccgctg ctgactttga tccagccatt gccataacgg
    64141 gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcacg
    64201 tcaatgctgc cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca
    64261 atcacctcac cgagtactgc attaagatcc gccatttcca tcggcatctc ctgcccggtg
    64321 cgcaggtagt cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc
    64381 gattctgcca gatagccatc ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc
    64441 agcggcgtgc gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt
    64501 tgcttaacac cagccgccat atggttaaag gcacgggtaa cggaacgcac ctcggacgcg
    64561 ccatactcac gcagcggcgg cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg
    64621 agatcgacca acggtcggtt ctggatacga ataaacagcc acgccccgcc tatcgccaat
    64681 agcataatcg ccagcgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc
    64741 agcggcacgc gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa
    64801 cttttgttga cctcaacgcg cacttccgtc gggccgccca gttgctgtgc catctgatgg
    64861 cttaagaatt catagtgttg cgcccaacgc agacctgcct cttcggcagc ctcgttggag
    64921 tagagagaga tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac
    64981 tgcgtgccgt cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg
    65041 actttattaa actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc
    65101 gtcaccaggc tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt
    65161 cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca
    65221 ggccccaaac ggtctgaatg taacgcggat gcgctggatc ttcttctacc atgcggcgca
    65281 gacgcgaaat ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa
    65341 ggttcatcag cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta
    65401 ccgcaaactc accgctggtg agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac
    65461 cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg
    65521 gcagttcgtt cgcctgacga cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt
    65581 taaacggttt tggaatgtag tcgtcagcgc caatctccag gcctacgata cggtccactt
    65641 cttccccttt cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc
    65701 ggcaaatcga caagccatct tcaccaggta acattaaatc cagtaccata agatggaaag
    65761 attcacgagt cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc
    65821 cttgttcggt gagataacgt tccagcagcg cacgcaggcg catgtcgtca tcgaccacca
    65881 gaatcttgta gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg
    65941 tgtattctta aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa
    66001 attgttacaa agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat
    66061 tgattctgtt gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga
    66121 atcaacgaga tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag
    66181 cttaattatc tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca
    66241 agtctgggcg accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa
    66301 atcgaccgtc gcgtgcgcta tctcactaaa tgcatggaaa atctcaaaat cgtcgattac
    66361 tcccctcagc aggaaggcaa ggtctttttt ggcgcatggg tggagattga aaacgacgat
    66421 ggcgtgactc accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac
    66481 atctctatcg attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg
    66541 gtggtgaata cccctgccgg ggaagcgagc tggtatgtca atgctatcga gtacgtgaaa
    66601 ccgtaaggaa gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat
    66661 aactatcccc tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc
    66721 tgccgcatta ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc
    66781 ctgcttgacg aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc
    66841 ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg
    66901 gaagagagac gtcaggcgat cctcaaatcc atttccgagc aaggcaaact caccgatgat
    66961 ctggcgaagg ccatcaacgc cacactaagc aaaaccgaac tcgaagatct ctacctgccc
    67021 tacaaaccta aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg
    67081 gctgacctgc tgtggagcga tccgtcacac acgccagaag tcgccgctgc acaatatgtt
    67141 gatgcagata aaggcgtggc agataccaaa gccgcgctgg acggcgcgcg ctatatcctg
    67201 atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag
    67261 aacgcgcatc tggtctcaac cgtgatgagc ggtaaagaag aggaaggggc aaaattccgc
    67321 gactatttcg atcatcacga accgctgtcc acggtgcctt ctcaccgcgc gctggcgatg
    67381 ttccgtgggc gtaacgaagg cgtactccag ctttcgctga atgccgatcc gcagttcgaa
    67441 gagccgccaa aagagagcta ttgcgagcaa atcatcatgg atcatctcgg cctgcgcctg
    67501 aacaatgctc cggcggatag ctggcgcaaa ggcgtggtga gctggacctg gcgcatcaag
    67561 gtgctgatgc atctggaaac cgaactgatg ggcaccgtgc gcgaacgcgc ggaagatgaa
    67621 gcaatcaacg tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg
    67681 cgtgccacga tgggcctcga tccgggtctg cgtaccgggg taaaagtagc ggtggtcgat
    67741 gccactggca aactggtggc gaccgatacc atttacccgc acaccggaca aacagcaaaa
    67801 gcagcgatga ccgttgctgc cttgtgtgaa aagcataacg ttgaactggt ggcgatcggc
    67861 aacggtacag cttcccgcga aaccgaacgt ttctatctcg acgtgcagaa gcagttcccg
    67921 aaagtgaccg cgcagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcggcttcc
    67981 gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggtgc ggtatctatc
    68041 gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc
    68101 gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact ggatgcagta
    68161 gtagaagact gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt tccgctatta
    68221 acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag
    68281 aacggccagt tcaagaaccg tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc
    68341 ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg ataacccgct ggacgcctct
    68401 accgttcacc cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca
    68461 ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact
    68521 gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt
    68581 cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac
    68641 gacctgcaac cgggtatgat cctcgaaggc gctgtcacca acgtcaccaa ctttggcgcg
    68701 tttgtcgata ttggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag
    68761 tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg
    68821 gaagtggatc ttcagcgtaa acgtatcgcc ctgaccatgc gtctggacga gcagcctggc
    68881 gaaaccaacg cccgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc
    68941 aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg
    69001 gcggcaatgg gcaaaaaacg ttaaacgccc tgattgaccc ctaaacctgc gcgataaggt
    69061 gtccacgccg catctggcaa cgaacgcctt gtctgacata aaagtgccgg agaatatctc
    69121 cggcattttt attccacagc caaactcaca atatattccg gcaacattta tcacttcatt
    69181 aacagctgaa accttaatta aacattagcc agtcctggta attcgctatt cgaattatat
    69241 tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta ttcaaaccaa
    69301 cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat tgtgctgttg
    69361 attatttaat ctctccttcg ttggcaaatc atctggtctc atgtcgctgt caaacgcccc
    69421 atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac actccagata
    69481 ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc
    69541 tttctcttgg catgttacct ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg
    69601 accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct
    69661 tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc
    69721 ataggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca gctcactggc
    69781 gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga agggcaattc
    69841 tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc
    69901 atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt gagtggcgac
    69961 gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg
    70021 ctacaactgc tggaactcgg cattccctgc atcgtggcac tgaacatgct cgacattgcc
    70081 gagaagcaaa atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg ctgcccagtg
    70141 atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc
    70201 tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact caacgaagca
    70261 gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc
    70321 ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat
    70381 ctggatgccg ccctcgcccg tctgcgtaat gagatggacg atccggcgct gcacattgcc
    70441 gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca
    70501 gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg
    70561 ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg
    70621 ttacagccgc tgtttgacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt
    70681 ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc
    70741 ggcattaaca ccgtgctgcc actggtgccg cagattggca tgatgtacct gttcctctcc
    70801 ttccttgagg actccgggta tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag
    70861 gcgctgggct tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta
    70921 ccgtcggtaa tgggtgcacg tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg
    70981 atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc
    71041 ttcttcgggc agaacggtgc gctggcggtc ttctcgctgt atatgctggg tattgtgatg
    71101 gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc gacgccgttt
    71161 gtcatggagt tgccggtcta tcatgtacca cacgttaaaa gcctgattat ccagacctgg
    71221 cagcgtctga aaggcttcgt tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc
    71281 ctgagcgctt tcaacagctt ctcgctgagc gggaaaatcg tcgataacat caacgactcg
    71341 gcgctggcgt ccgtcagccg ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa
    71401 gataactggc aggcaacggt tggcctgttt acaggtgcca tggcgaaaga agtggtagtg
    71461 ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggca
    71521 gagtttaacc tcggtgaaga gctgttcagt gcgatagatg aaacctggca gagcctgaaa
    71581 gacaccttca gccttagcgt actgatgaac cccattgaag ccagcaaagg cgacggcgaa
    71641 atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc agcagcttac
    71701 agctacctga ttttcgtcct gctgtatgta ccatgtatct cggtgatggg agccatcgcc
    71761 cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac
    71821 tcgctggcaa cattgttcta tcaggtcgcc agctacagtc agcacccaac ttacagcctg
    71881 gtgtgcattc tggcggttat cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg
    71941 cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagcag ttgctgcgca
    72001 gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtgcgc gatttgctgg
    72061 cgttacgggg ccgtatggaa gcggcccaga taagccagac attgaacact ccacagccaa
    72121 tgattaacgc catgctgcaa caactggaaa gtatgggcaa agccgtgcgg attcaggaag
    72181 aacctgacgg ctgcctctct ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc
    72241 gcgagtggtg ggcgctgcgt taaccttact ccatcgcctg ttttggaaag cccggtatgc
    72301 gtctgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaactct tgcagcacgg
    72361 cgtaagttcc tttgaaagca tctcgcaggg atgaaaactc gctaatacac aggtgtggag
    72421 tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag caaaaagcag
    72481 agttccaccc cacacgatgc gctgttcaaa ctctttttac gccaaccgga cacggctcgt
    72541 gattttcttg cgtttcattt accggcaccc attcacgcgc tttgtgatat gaaaaccctc
    72601 aagctggagt cgagcagctt tattgatgac gatctgcgtg aaagctattc cgatgtgctg
    72661 tggtcggtga aaacggaaca aggaccagga tacatctatt gtctgattga acatcaaagc
    72721 acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc aatgcaaaat
    72781 caccttgatg ctggatacaa aacgttgccg atggtggtgc cattgttgtt ttaccacggt
    72841 attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaaactg
    72901 gcaaggcagc tttatgcctc cgcatttccg ctgattgatg tcaccgtcat gcctgatgat
    72961 gaaatcatgc agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa
    73021 cgcgatctga tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat
    73081 gacagacaaa tcaaagggct gtttaattac atactgcaaa ccggcgacgc ggtacgtttt
    73141 aacgatttta tcgacggcgt tgccgaacgt tcaccgaaac acaaggagag tttaatgaca
    73201 attgcggaaa gattgcggca ggagggggaa caatccaaag ccctgcatat agccaaaata
    73261 atgcttgaat ccggagtccc tcttacagac atcatgcgct ttaccggggt gtcagaagaa
    73321 gagttggctg cggcgagcca gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc
    73381 tctgcttcaa cgccaccagc aggtgacaaa actcgaccgg atgcgaaata aacggcgcat
    73441 gggccgcttt ggcgaagata tatgattcgc tgtgaggcca aagtttatcc agcatcggca
    73501 ccactttgcg cggcaccaga ccgtcgagat agccatacaa tcgcaaaaac ggcatggaca
    73561 cgttttgcag cggctgacgg agatcgaccg ttttcaggat ttccagcccg ccattaagca
    73621 cgtcaacctc cggcatcggt aacgccagaa cggttttctt caacgcccgc gcatcctggc
    73681 gcgccgtttc agtccccatg gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat
    73741 catcactgag ttgctgctga aatcccgcca gtacgtccgg ttttatcccc ggccactcgt
    73801 cacgagcact aaaacaaggt gacgacgcca cggtgaccag cgcctgaaca cgctcgggat
    73861 gggttaaaac aatttggctt gccaccagcc cgcccagact ccagcctaac caaatggctt
    73921 tatcaggtgc ctgttgcagc acggcttcgg ccatatcagc aagtgacagc gcaccaaatc
    73981 cccggctacg cccgaagccg ggcaggtcaa caaggtgcag cgtaaaatgc gagctaagtt
    74041 cctcgtcaat gcaacgccac acttcggcat tcagtcccca tccgtgcagc agcacaagat
    74101 gaacattccc ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt
    74161 tgacttacaa ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc
    74221 cactggcgtt aggtcattgg gggatttgtt cggtctgttc acgcgccacc cgcacagata
    74281 aaacgttatg cccacaatgt ggattacccg ccacacactc ccatcttccc tgcggtcgct
    74341 gcctgcaaaa accgccgccc tggcaaagac tggtcacggt tgccgactat gcgccgccgt
    74401 taagtccgct tatccaccag cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt
    74461 cacgtctgtt actattggaa gtcttacacg ctcgtcgcac caccgggttg caattgccgg
    74521 atcgcatcat cagcgttccg ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga
    74581 gcgatttgct gtgtcagccg ttatcacgct ggttgcattg ccaatgggat agcgaagccg
    74641 tcacacgtac acgggccact gcgacccagc attttctcag tgcccggctg cgcaagcgca
    74701 acctgaaaaa tgcctttcgt cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg
    74761 atgatgtcgt taccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg
    74821 gtgcggcgac tgtccaggtc tggtgccttt gtcgaacctt gtagagcctc gatgatgggc
    74881 gtattataac caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat
    74941 gctgcacaag cgcactttgc caaactgctg gcaaatcagg aagaagggac acaaatccgc
    75001 gtatttgtga ttaaccctgg cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg
    75061 gatgccgtgg aagccaccga cactgccctg aaatttgacc tgctgaccgc gtatgttgat
    75121 gagttaagcg caccatacct ggaagatgca gagatcgact ttgttaccga ccagttgggt
    75181 tctcagttaa cgctgaaagc cccgaacgcc aaaatgcgta aagtggcaga cgatgcaccg
    75241 ctgatggagc gcgtggagta tatgctgcag tcgcagatca acccacagct tgctggtcac
    75301 ggtggtcgcg tttcgctgat ggaaatcacc gaagacggtt acgccattct gcaatttggc
    75361 ggcggctgta acggttgttc catggtcgat gtgacgctga aagaagggat cgagaagcag
    75421 ctgctgaacg aattcccgga gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc
    75481 gaacactcct actactaagt tatcccctct tttgtagatt gcccgatgcg acgctaaagc
    75541 gtcatttcgg gcctacgttg aacatgtgcc agacgttggc acagcattgc tttggcaaat
    75601 ccctcccttc gtttccccgc gataatatga ccaacctctc ataatttaaa tttaccccgc
    75661 tctggtgatt ctcaaacgcc agatgttacc cgtatcattc acatgggtac caaacatact
    75721 cctgacatct gactacaata attagtttta gtgggtatca gtcgtggtgc cgcaatatct
    75781 ctgttcccga ttgggataat tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa
    75841 tgaaaggcca ggtaaatcat gccattagtc attgttgcta tcggtgtaat cttgttgttg
    75901 ctcctgatga tccgcttcaa aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct
    75961 gttggattaa tgcaaggaat gccgctggat aaagttattg gctccatcaa agccggtgtc
    76021 ggcgggacgc tcggtagcct tgccctgatc atgggttttg gcgcaatgct gggcaaaatg
    76081 ctggcagact gcggtggcgc acaacgtatc gccaccacgc tgattgccaa atttggtaaa
    76141 aaacacatcc agtgggcggt ggtactgacc ggttttaccg ttggttttgc cctgttctat
    76201 gaagtgggct ttgtgctgat gctgccgctg gtgtttacca tcgcggcttc tgcgaatatt
    76261 ccactgttgt atgttggtgt accaatggca gctgcactgt ctgtgaccca cggcttcctg
    76321 ccaccgcatc cgggtccgac tgcgattgcc accattttca atgccgatat gggtaaaacc
    76381 ctgctgtacg gtactattct ggcaatcccg accgtgattc tcgccggtcc ggtttacgct
    76441 cgcgtgttga aaggtatcga taagccaatt ccggaaggtc tctacagcgc gaaaaccttc
    76501 agcgaagaag agatgccgag ctttggcgtc agcgtctgga cctctctggt gccggtagta
    76561 ctgatggcga tgcgtgcgat tgccgaaatg atcctgccga aaggtcacgc tttcctgccg
    76621 gtagcggagt tcctcggtga cccggtaatg gctacgctga ttgccgtgct gattgccatg
    76681 ttcacctttg gtctgaaccg tggtcgttca atggctcaga ttaacgacac gctggtttct
    76741 tccatcaaaa tcattgcgat gatgctgttg atcatcggtg gtggcggtgc gttcaagcag
    76801 gtgctggtag acagcggcgt ggacaaatac attgcttcca tgatgcacga aaccaacatt
    76861 tctccgctgc tgatggcctg gtcgattgct gccgtactgc gtatcgcgct gggttccgca
    76921 accgttgcgg caatcactgc gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt
    76981 agcccggagc tgatggttat tgcggtgggt tccggtagtg tgattttctc tcatgtgaac
    77041 gatccgggct tctggctgtt caaagagtac tttaacctga ctatcggcga gaccatcaaa
    77101 tcctggtcga tgctggaaac cattatctcg gtgtgcggtc tggtaggctg tctgctgctg
    77161 aatatggtga tttgataaca ctgactgccg gatgcatcgc atccggcatc aaccgcactc
    77221 tacttcttct tcgctgcagc tctgcgccgt ctgtccaaat ccttcagcaa cttgttcacg
    77281 ccatcatcgg caaacatcga ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac
    77341 tggtaactgg tgccaggaat attcaccggt tcggccatat ccagccagtc ttccggctgt
    77401 agtcctaaca gagcactgtt actgtcggca atgtagcgct gcaaaccacg gttcagcgtc
    77461 ggcgtcatcg acatcaacga tgccttatgc ccggcacgtt ttggcagaca accatattta
    77521 tgcagtgcat ccagcagccc ttgcttcgcc agttcgcgat cctgatacag accgcgcagt
    77581 accacttcat ccggatacag ccccagggtt ttgcccagcg ttagatcccc gctctcccag
    77641 taaccgcgta gcgtaggcag gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac
    77701 gctttcggtg cacggaacgt cttctcgtgg tcgttttcga aatagagcac tttgtaggag
    77761 tacacaccgc tgctgcgcag cttaccgaca atctctaccg gtacggtacc gagatcttca
    77821 ccaatcacca tacagcgatg acgtttactt tcgagtgcca gaatcgagag cagatcatcc
    77881 accggatagt gaacatacgc gccctgatct gccgtctcgc catacggtat ccaccacaaa
    77941 cgcagcatcg acatcacatg gtcaattcgt aatgcgccgc agttttgcat attggcacgc
    78001 aacagctcga taaacggttc ataggcacgc gcggtgatga tatgcgggtc cattggcggt
    78061 aatccccagt tctgccccaa cgggccgagg atatccggcg gcgcgccaac cgacgctttc
    78121 aggcaatata gttcacggtc acaccaggtt tccgccccac cttccgctac gccaaccgcc
    78181 agatcacgat acaagccaat cggcatttca tagccctggc ttatctccca gcaggcggca
    78241 aactggctgt aagccagcca ctgcaaccag agataaaaat cgacgtcatc acgatgttct
    78301 tcgcagaact gacgcacttc tggtgaatcc acgttctgat acatctctgg ccatgcaggc
    78361 cagccccagc gcatttcgtc ctctttcact tgctgggcat gtagcgcatc aaaggctgcc
    78421 tgccagaaca ggctgtcgcc ctgctctgca acaaactggc gaaacgcggt catctgctca
    78481 tcatcacgtt gcgcgaaacc tttccacgcc attcgtaatg ctgtcatttt tagggcggta
    78541 accgtggagt aatcgaccca gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc
    78601 ggcaactgcc accaggcctg agcctcttcg ctaagatgga aatcttcaac ggcgttaacg
    78661 tcgatataaa tcacattcag ccaacggcga gaagacgggc tgtatgggct ggcactctcc
    78721 ggatttgccg gatagagcgc atgaatcggg ttcaggccga tgaacgaccc gccacgtttt
    78781 gccacatcca ccagcatcgc tttgagatcg ccaaaatccc caatacccca gtttttttcc
    78841 gatcgcagcg tataaagctg aacgcaggca ccccacagct tttgtttatt cagcaacgcc
    78901 tgcggttcgt aacagcgttt cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc
    78961 tgggtgagtg tcagcgtgtg ataaccttcc ggcagcttcg tcggtagatt gaacgctttg
    79021 ccccccgtta catggccttt gtactgtgtt ccttcttcgg tggtcagcag ccagctatat
    79081 tcgccgctgc cctccaccac catcggcatt tttttgccgc tggtataaac catgacattc
    79141 gggactggcg ttaccgccac tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc
    79201 cgtttggttt cggcgctaat cgactgcggt ttaccgtggg cattgatgta attggggcta
    79261 atccccgccg ccagcgcggc attatccaga cgtttgcttt ccatcgagct tccttagcgt
    79321 tttgcctgcc agatacgagc ctgataatcg cgaatagagc gatccgagct aaacataccg
    79381 cagcgggcgg tattgaggat cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca
    79441 tccacctgct tttgtgcctc aacataggct gcgaaatccg ccatcaccag atacggatcg
    79501 ccgccttgtt tgccgatact gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg
    79561 tatttaccgc tttccagctc tttcaatact gcgtccagca ccttatcttt cttccgccat
    79621 ttcaccgggt cgtagccttt ggccagaata gccttcactt gttccacggt atgaccaaaa
    79681 ataaagatat tttcttcacc gactttctcg gcgatttcaa cgttcgcacc atccagcgta
    79741 ccgacagtaa gcgcaccatt gagcgccagt ttcatattgc cggtaccgga agcttcttta
    79801 cctgcagtcg aaatttgttc ggagatatcc gccgccggga tcagtttttc cgccgccgaa
    79861 acgcaataat ccggcaggaa caccaccttc aacttatcgc caaccagtgg atcgttgttg
    79921 atcacgtcag ccactttgtt gatcgcaaag ataatattct tcgccaggta gtagcccggt
    79981 gccgctttcg cgccgaagag gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga
    80041 atttctttgt acaacgccag aatatgcagc agattcaggt gctggcgttt gtactcatgc
    80101 agacgtttga tctgaatatc gaaaatcgcc tgtggattga tctcaatacc ggtacgaact
    80161 ttcacaaact ccgccagacg gactttattc gcctgcttga tctcgcgata ttgctgacgg
    80221 aatttcgcat catcagcgaa tttttccaga ttgatcagct gatcgagatc gttagcccac
    80281 tctttttgca gtgatttatc caacagagcc gccagtgcag ggttgcactg tttgatccag
    80341 cgacgtgggg taataccgtt ggtgacgtta tggaatttgt tcggccatag ctggtgatat
    80401 tccgggaaca gatctttcac caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg
    80461 aaaccgccaa ccacacacag gttcgccata tgcacttgtt tatcgtgcac caccgccagt
    80521 ttggcccaca ctttttcatc gcccggccag gttttctcca ccagcgtttt aaagcgagta
    80581 ttaatttcgt taataatctg catatggcgc ggcagtaagc ctttcaccag tttcacatcc
    80641 cagcgttcca gcgcttctgg catcagggta tggttggtgt aagcgaaagt tttgctggta
    80701 atggcccagg cgtcatccca gctcatctgg tgctcatcga tcagcacgcg cagcagttct
    80761 ggaatcgcga tagttgggtg ggtatcgttc agctgaataa cttcgtaatc cgccagttcg
    80821 tgcagtttac gccccgccag atgatggcga cgcaaaatat ccgctaccga acaggcacac
    80881 tggaagtact gctgcatcaa gcgcagcttt ttaccggcag tatggttgtc gtttggatag
    80941 agaactttgg tcagtttttc cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg
    81001 tcgttaaatt tagtcagatc aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc
    81061 tgcgccacgc cattacgata gccgacaacg gggagatccc acgcttgacc ggtaatggta
    81121 aactccggct cccagcgtcc gtctttcgtc actttaccgc caatccctac ctgcacatcc
    81181 agtgcttcgt tgtggcggaa ccacgggtag ttactgcgat gccagtcatc cggcgcttca
    81241 acctgtttgc catcgacaaa agactggcgg aacaaaccat attgatagtt cagaccgtaa
    81301 cccgtcgcag actgaccgac agttgccatt gagtcgagga agcacgccgc cagacgtccc
    81361 agaccaccgt tacccagcgc cgggtcgatc tcttcttcca gcaggtccgt cagattgatg
    81421 tcataagcct tcaacgaatc ctgtacatcc tgataccagc cgagattcaa caggttgttg
    81481 cccgtcaggc gaccaatcaa aaactccatt gagatgtagt taacatgtcg ctgattcgcc
    81541 accggcttgg cgaatggctg agcacgcagc atttcggcca gtgcttcact cactgctagc
    81601 caccactggc gaggagtcat ttcagccgca gaatttaagc cataacgctg ccactgacgt
    81661 gaaagcgctt cctgaaattg cttatcgtta aaaataggtt gtgacatagg agttccactt
    81721 ttcttagatt ttcaacacaa cgttatcgct agtttgccag gctcgatgtt gaccttcctc
    81781 atcctgcggg ggattaggca gggaggagtt gcggggatga gcaaggaaat gtgatctcaa
    81841 ccacttaaag ctagtgcaaa ccacaggatt agcagcaaat caatgcaata cagcgcagaa
    81901 aatctgtatc taagtgcaaa aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc
    81961 tataaattaa gtaaaggagt gaaacagttt cataagtaaa atatccagtg tgctccatct
    82021 cattcttaat agatttatta agatcatctt tttagatggc actttcatca ggaatgaaga
    82081 agaaaccctt gcttaaatga atctgatgaa cataagggaa accagtattc acgctggatc
    82141 agcgtcgttt taggtgagtt gttaataaag atttggaatt gtgacacagt gcaaattcag
    82201 acacataaaa aaacgtcatc gcttgcatta gaaaggtttc tggccgacct tataaccatt
    82261 aattacgaag cgcaaaaaaa ataatatttc ctcattttcc acagtgaagt gattaactat
    82321 gctgattccg tcaaaactaa gtcgtccggt tcgactcgac cataccgtgg ttcgtgagcg
    82381 cctgctggct aaactttccg gcgcgaacaa cttccggctg gcgctgatca cgagtcctgc
    82441 gggctacgga aagaccaccc tcatttccca gtgggcggca ggcaaaaacg atatcggctg
    82501 gtactcgctg gatgaaggtg ataaccagca agagcgtttc gccagctatc tcattgccgc
    82561 cgtgcagcag gcaaccaacg gtcactgtgc gatatgtgag acgatggcgc aaaaacggca
    82621 atatgccagc ctgacgtcac tcttcgccca gcttttcatt gagctggcgg aatggcatag
    82681 cccactttat ctggtcatcg atgactatca tctgatcact aatccagtga tccacgagtc
    82741 aatgcgcttc tttattcgcc atcaaccaga aaatctcacc ctggtggtgt tgtcacgcaa
    82801 ccttccgcaa ctgggcattg ccaatctgcg tgttcgtgat caactgctgg aaattggcag
    82861 tcagcaactg gcatttaccc atcaggaagc gaagcagttt tttgattgcc gtctgtcatc
    82921 gccgattgaa gctgcagaaa gcagtcggat ttgcgatgac gtttccggtt gggcgacggc
    82981 actacagcta atcgccctct ccgcccggca gaatacccac tcagcccata agtcggcacg
    83041 ccgcctggcg ggaatcaatg ccagccatct ttcggattat ctggtcgatg aggttttgga
    83101 taacgtcgat ctcgcaacgc gccattttct gttgaaaagc gccattttgc gctcaatgaa
    83161 cgatgccctc atcacccgtg tgaccggcga agaaaacggg caaatgcgtc tcgaagagat
    83221 tgagcgtcag gggctgtttt tacagcggat ggatgatacc ggcgagtggt tctgctatca
    83281 cccgctgttt ggcaacttcc tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc
    83341 ggaaatccac cgtgccgccg cagaaagctg gatggcccag ggatttccca gcgaagcgat
    83401 tcatcatgcg ctggcggcag gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc
    83461 ctggagtttg ttcaaccata gcgaactgtc gctgctggaa gagtcgctta aggccctgcc
    83521 gtgggacagt ttgctggaaa atccgcagtt agtgttattg caggcgtggc taatgcaaag
    83581 ccaacatcgc tatggtgaag ttaacaccct gctagcccgt gctgaacatg aaatcaagga
    83641 catcagagaa ggcaccatgc acgcagaatt taacgctctg cgcgcccagg tggcgattaa
    83701 cgatggtaat ccggatgaag cggaacggct ggcaaaactg gcactggaag agctgccgcc
    83761 gggctggttc tatagccgca ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa
    83821 aggcgaattg acccgctcac tggcgctaat gcagcaaacc gaacagatgg cacgccagca
    83881 cgacgtctgg cactacgcct tatggagttt aatccagcaa agtgaaattc tgtttgccca
    83941 agggttcctg caaaccgcgt gggaaacaca ggaaaaagcg ttccagctga tcaacgagca
    84001 gcatctggaa cagctgccga tgcatgagtt tctggtgcgc atccgagcac aactgttatg
    84061 ggcctgggca cggctggatg aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc
    84121 gtcttatcag ccacagcaac agcttcagtg cctggcaatg ttgattcaat gctcgctggc
    84181 ccgtggtgat ttagataacg cccgtagcca gctgaaccgt ctggaaaacc tgctggggaa
    84241 tggcaaatat cacagcgact ggatctctaa cgccaacaaa gtccgggtga tttactggca
    84301 aatgaccggc gataaagccg ccgctgccaa ctggttgcgt catacggcta aaccggagtt
    84361 tgcgaacaac cacttcctgc aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct
    84421 gggcgagttt gaacctgcgg aaattgttct cgaagaactc aatgaaaatg cccgcagtct
    84481 gcggttgatg agcgatctca accgtaacct gttgctgctt aatcaactgt actggcaggc
    84541 cggacgtaaa agtgacgccc agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac
    84601 cggatttatc agccattttg tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct
    84661 gattcaactc aatacgctgc cggaactgga acagcatcgc gcacagcgta ttctgcgaga
    84721 aatcaatcaa catcatcggc ataaattcgc ccatttcgat gagaatttcg ttgaacgtct
    84781 gctaaatcat ccggaagtgc cagaactgat ccgcaccagc ccgttgaccc aacgtgaatg
    84841 gcaggtcctg gggctgattt actccggtta cagcaatgaa caaattgcgg gcgaactgga
    84901 agtggcggca accaccatca agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc
    84961 ccaccgtcag gctgccgtgc agcacgcgca gaaactgctg aagatgatgg gttatggggt
    85021 gtgagtttag ccggataatg cattggatcc ggtttacatc tttacatcat tcaatactca
    85081 cccgcgttac gccatctgtt tctatcaaac caaaccgcac cggcaagaaa cgctccacca
    85141 ccgcgatatt ggtcagcaga tggcatgaag gatgggcgac cttaaattct cccgcgcccg
    85201 ccagcgccat cggcagtacc agttggtcgg cgagatattc ccccaccgcc gctgggcttg
    85261 ccaggtagcg tttcacctct ttcactaact gcgcggctac cacctcggca ctgacgcgct
    85321 tttcaccgac gacgaaaaag cgttcagtga tattttcact ttcgacttcc agcgagacgg
    85381 tattacccgg cccctggtcg cgcggcaggt tatgaatatt ctgttcatgc agggaaaaac
    85441 tccccgccag tgtagcgatt tcacgctcag caacatggcg cggcacacca gctaatagaa
    85501 cttctccacg catctgcaca atgttcccgc gctcgccaag ttgcaaggtg ttaaacgatg
    85561 ccacgggcga gacttccgtt gccaccacgc cgcctccggc aggataaaaa ccgtgacgta
    85621 acagcgtggt ttgctgatga attcctattt tcgccagcag cggctccagc acccggcgga
    85681 taaaatccgc aggcggggcc gacgggttat cggtgcctcc gctcacttca acacgcgaag
    85741 gtccatcggc aaaccacagc gcgggcagca ccgtttgcag caccagcgta cagcttccgg
    85801 cactaccgat agcaaagcgg tactcgccgc cgcgcacggt gccaggccgg aagaccagac
    85861 gctgcgaccc cagctccgcg ccttccaccg ttgccctaca aatttccgca gccgctttta
    85921 ccgcggtcag atgctggcgc aacagccccg gtttcgcccg cccggcacga atgctggtga
    85981 tggtaaatgg ctggccggtt atcatcgaca ggctcagcgc cgagcgcagg atctgcccgc
    86041 caccttcgcc ctgtgcgcca tccagcgcaa tcatcctttt catcatttat ccttttacgc
    86101 acaccacctg acgcagggta tagataactt ccaccagatc gctttgtgcc gccatcaccg
    86161 catcaatatc tttatacgcc atcgggattt cgtcgatcac ttcggcatct ttacggcatt
    86221 ccacatgcgc ggtggcacga atttgatctt ccacgctgaa cagttttttc gctttagtac
    86281 ggctcattac ccgcccggca ccgtggctgc acgaacagaa cgactcttca tttcccagcc
    86341 cacggacgat aaagcttttt gctcccatcg aaccgggaat aattccatat tgaccagcac
    86401 gcgcagacac cgcgccttta cgcgtcacgt agatctcttc accaaagtgc tgttcttttt
    86461 gcacatagtt gtggtgacag ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg
    86521 ttttctgcgt aatgctctgc aacgccgtta ccacgttttc catcatcgca tcgcggttaa
    86581 ggctggcgaa aagctgcgcc caggccacgg ctcttaggta atcatcaaag tattccgtac
    86641 cttccataaa gtacgccagg tcgcgcgatg gcaacgtttc aagctggtcc tgcatctctt
    86701 tttgtgccaa atcgataaag taagtcccga tggcgttacc aattccgcgt gaaccggagt
    86761 gcagcataat ccacacctgg tccgactcat caaggcagat ttcaataaag tggttacccg
    86821 ttcccagcgt tcccaggtgt ttatagttgt tagtgttaag gaaacgcgga tatttttgcg
    86881 ttaaccactg ataaccggct tcaagctcag cccatttcgc atcgacgtta acaggcgggt
    86941 tttcccatgc gcctttatca cgtttacaac ggccagtggt acgtccgtgc ggcaccgcag
    87001 tttcaatcgc ctgacgcagc tctgccaggt tttcaggcag gtctgccgcc gttaacgcgg
    87061 tacgcagcgc gttcattcca cagccaatat ccacgcccac cgccgccgga ataatcgccc
    87121 ctttggtcgg gatcacgcta ccaatggtgg aacctttacc caggtgtaca tcaggcatta
    87181 ccgcaatatg tttgaaaata aacggcatct tcgccgtatt aataagttgc tgacgcgcat
    87241 cggcttctac cggcacgcct ttggtccaca tttttaccgg ggcattttca gtggtcagta
    87301 attcgtaatt cattttgttt ttctcttttc gttgtttgct gtcgtgataa ttgcaaccgt
    87361 cgtgccagaa aattaaataa gcagattaat tttttaattc attgttttta aataggttaa
    87421 atctttacgt ccgtaaacgg agattttcca caaaaccaat tcaccgataa tgaaatatcg
    87481 tcttttataa ggatatctaa gatgcgtaaa acagtggctt ttggctttgt cggtaccgtg
    87541 ctggattatg ccggacgcgg cagtcagcgc tggtcgaaat ggcgtccgac gctttgttta
    87601 tgccagcaag aatcgttagt catcgatcgg ctggaattgt tgcacgacac ccgctcgcgc
    87661 tcgctattcg aaacgcttaa acgcgatatc gccagcgttt cgccagaaac acaagtcgtg
    87721 ggcgttgaga ttgaactgca taacccgtgg gatttcgaag aggtttacgc ctgcctgcat
    87781 gatttcgccc gtggttacga gtttcagcca gaaaaagaag actatttaat tcacatcacc
    87841 accggcaccc acgtcgcgca gatttgctgg tttctgctgg cggaagcgcg ttacctgccc
    87901 gcccggctga tacaatcttc acctccgcgc aaaaaagaac agccgcgcgg cgcaggtgaa
    87961 gtgacgatta tcgatctcga tttaagccgt tataacgcca tcgccagccg ctttgccgag
    88021 gaacgccagc aaacgcttga ttttcttaag tccggcattg ccacgcgtaa cccccacttc
    88081 aaccgcatga ttgagcagat cgaaaaagtg gcgatcaaat cccgcgcgcc gattctgctt
    88141 aacggtccaa ccggcgcggg caagtcattt ctggcgcgac gcatcttcga gttaaaacag
    88201 gcgcggcatc agtttagcgg cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat
    88261 accgccatgt cgacgctgtt tggtcatgta aaaggcgcgt ttaccggggc gcgggaatct
    88321 cgtgaaggtt tattacgcag cgccaacggc ggaatgttgt ttcttgatga gattggcgaa
    88381 ctgggcgcag acgaacaggc aatgctgcta aaagccattg aagagaaaac cttttacccg
    88441 tttggcagcg atcgccaggt gagcagtgat tttcagctta tcgccggaac ggtgcgcgat
    88501 ttgcgccagc tggttgccga aggcaaattt cgcgaagatc tgtacacgcg gatcaatctc
    88561 tggaccttca ccctgccggg tctacgccag cgccaggaag atattgaacc gaacctggat
    88621 tatgaagtgg agcgccacgc ctcactcact ggcgacagcg tgcgttttaa caccgaagcg
    88681 cggcgcgcct ggttggcttt tgcgacctct ccccaggcaa catggcgcgg taactttcgc
    88741 gaactttctg ccagcgtcac gcggatggcc acctttgcca ctagcggacg catcactctg
    88801 gacgtggttg aagatgagat aaaccgtctg cgctataact ggcaggagag ccgcccctcc
    88861 gcgcttacgg cgttgctggg cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa
    88921 ctggaacacg ttatcgctat ctgccgccag gcaaagtcgc tttccgcagc cggacgccag
    88981 ctttttgacg tttcgcgcca gggcaaagcc agcgtcaatg acgcggatcg gctacgcaaa
    89041 tacctggcgc gttttggtct gacgtgggaa gccgtgcagg atcagcacag ctccagttga
    89101 atatggtggt ccgtcagcac ctgcatcacg cttactggcg gcggggcgtc ggtgtagacg
    89161 gcatctacca tgctgatgct gcccatattg accatcgcgt tacggccaaa tttcgagtga
    89221 tcgacaacca gcataacgtg gcgcgagttc tcaataatgg cgcgtttggt gcgaacttcg
    89281 tggtaatcga actccagcag cgagccgtcg ctatcgatgc cgcttatccc cagaatgccg
    89341 aaatcaaggc ggaactggga gataaaatcg agcgtcgctt cgccaatgat cccgccatcg
    89401 cggctgcgta attcgccacc ggcgagaata atgcggaaat cttcttttac catcaacgtg
    89461 ttagcaacat tgagattgtt ggtgacaata cgcaaattgc tgtgattaag cagcgcgtgc
    89521 gccaccgctt ccggcgtggt gccgatatcg ataaacagtg tcgagccatt ggggatttgc
    89581 tccgccactt tgcgggcaat cctctctttt tcttcggtct gggtggcttt acgatcgtgc
    89641 cacggcgtgt taaccgaact ggaaggcagc gccgcaccgc catggtgacg caggatcagg
    89701 ttttgctccg ccagctcatt gaggtcgcgg cgaatagtct gcgggctgac ggagaaatgc
    89761 tctaccagct cttcagtact gacataaccc tgctgtttaa ccagttcgat aataccgttg
    89821 tgacgttgtg tttgtttcat ttataaatcc ctggaattat tttcgttttc gcgcattgag
    89881 cgaatcaaca aaagccatcg ctaaacccac ggctaacccg gcgatgtgtg ctccgttcgc
    89941 catcgacatc ccaaacaaat caaaccatcc ggcgacaatc cagatcagcg caaagataat
    90001 taacccacgt tgcaggtaaa tgccactttg cggatcgcgt tcgccacgta gccagacgta
    90061 gcccatcagc gcatacacca cgccagaaag cccgccaaac cacggcccgc tgaatttttg
    90121 ctgcacatag ccgcttaaca gggcgctaat gagagtaata acaattagct taccgctacc
    90181 gaggcgtttt tccaccgcac cgccgagata ccaccaccag agcaggttaa agaggatatg
    90241 catcagcgag aagtgcatta acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt
    90301 tggatcgaat ggccaggcca gccataacat cacttcctga tcgccgagaa tttgcatggc
    90361 gataaacacc accacgcagg cgatcatcac cacccaggtt accggacctg cgcgttcacg
    90421 caaggcagca aagaaaggat aacggcgata atgcagacca ctgccggtat ggcccgcctg
    90481 ccagctggcc gccagataac gcggatctgc cgggttttcg agaaaacgcg ccagctccgc
    90541 ccgcacgcgc tcggcctggg actcatccgc cagccagaca tcgctttggt tatgttgttg
    90601 aatcgtgagg ataacaccct gcgtcgccat ataatcaaca aacgcctgcg ccacgcgggg
    90661 gttagcaaaa gaggtaatca tcaacatcgt tgctgtcgct tattccacac aaaaggggac
    90721 agtataaagc gttacgcgcc gtacgccacc tctgcgggaa actgacgctg ccaggcttca
    90781 aaaccgccgt caatgctata gaccacatcg tagccctgtt gcagcagata ctgcgccgcg
    90841 cctttgctgc tattgccgtg ataacacatc accatcaccg gagtgtcaaa gtcgttatca
    90901 cgcataaacg cgcccagcgt gtcgttggtt aaatggaaag cctgcaccgc atgccccata
    90961 gcgaaactct gtggatcgcg aatatcgacc agcaccgcct ctttttcctg caacttctgg
    91021 tgcgcgtcgg caacgttaat acattcgaac agatccatgc gtctctcttt ctttacaaac
    91081 aagtgggcaa atttaccgca cagtttacgt cgaagcggca gataaacgcc ataatgttat
    91141 acatatcact ctaaaatgtt ttttcaatgt tacctaaagc gcgattcttt gctaatatgt
    91201 tcgataacga acatttatga gctttaacga aagtgaatga gggcagcatg gaaaccaaag
    91261 atctgattgt gataggtggc ggcatcaatg gtgctggtat cgcggcagac gccgctggac
    91321 gcggtttatc cgtgctgatg ctggaggcgc aggatctcgc ttgtgcgacc tcttccgcca
    91381 gttcaaaact cattcacggt ggcctgcgct accttgagca ctatgaattc cgcctggtca
    91441 gcgaggcgct ggctgaacgt gaagtgctgc tgaaaatggc tccgcatatc gccttcccga
    91501 tgcgttttcg cctgccacat cgtccgcatc tgcgcccggc gtggatgatt cggattggtc
    91561 tgtttatgta cgatcatctg ggtaaacgca ccagcctgcc gggatcaacc ggtttgcgtt
    91621 ttggcgcaaa ctcagtgtta aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt
    91681 gggtagacga cgcccgtctg gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg
    91741 aagtgcttac tcggactcgc gccacctctg ctcgccgcga aaacggcctg tggattgtgg
    91801 aagcggaaga tatcgatacc ggcaaaaaat atagctggca agcgcgcggc ctggttaacg
    91861 ccaccggccc gtgggtgaaa cagttcttcg acgaggggat gcatctgcct tcgccttatg
    91921 gcattcgcct gatcaaaggc agccatattg tggtgccgcg tgtgcatacc cagaagcagg
    91981 cctacattct gcaaaacgaa gataaacgta ttgtgttcgt gatcccgtgg atggacgaat
    92041 tttccatcat cggcaccacc gacgtagagt ataaaggcga tccgaaagcg gtgaagattg
    92101 aagagagtga aatcaactat ctgctgaaag tgtataacgc gcactttaaa aaacagctta
    92161 gccgtgacga tatcgtctgg acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg
    92221 actcgccgca ggctattacc cgtgattaca cccttgatat ccatgatgaa aacggcaaag
    92281 caccgctgct gtcggtattc ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg
    92341 cgctggaaaa actgacgccg tattatcagg gtattggccc ggcgtggacg aaagagagcg
    92401 tgctgccggg tggtgccatt gaaggcgacc gcgacgatta cgcagctcgt ctgcgccgtc
    92461 gctatccatt cctgactgaa tcgctggcgc gtcattacgc acgcacttac ggcagcaaca
    92521 gcgagctgct gctcggcaat gcgggggcga taagcgacct cggggaggat ttcggtcatg
    92581 agttctacga agcggaactg aaatacctgg tggatcacga atgggtccgc cgcaccgacg
    92641 atgccctttg gcgtcgcaca aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg
    92701 tgagtcagtg gctggtggaa tatacgcagc agaagttgtc gctggcgtcg taaggatgat
    92761 gcccggttga agtgcaaccg ggctatttgg attccttaga ctattcattg taaataattg
    92821 aataacggcc cctgagctaa aatcgccaaa cttaagcacg ttacccaatc gccccacttc
    92881 ttcctctggt ttctattttt tctgcaccaa tggcaataat atccccatgc aaaaccaagc
    92941 tgtgcactaa tcgggccgac aaaatcagtc caaggctgcg gccaaaaagg ggaaaaattc
    93001 aggggaggcc agacaaacgt cagaaagaaa tttcggatat tttccagcaa cgctgatttc
    93061 tctggagaaa tataaagaat gaaattcgag agaattaacc agaaatagac cggcgaaaag
    93121 accagccagc aatgcagtct gttttttaat tcaggaggcg ttctgcggga gaattgccaa
    93181 ccgatgccag ccattacact gaaaaaaagt gcatattcaa ccattaactc aagttgtaat
    93241 tcctgaagag aaatacgcac aaccaggagc ggaaaggaga atattatttc tgccagaaaa
    93301 gttaacgtga atataagtac ggctaagaca ataaccgtcg gccataccaa taatggcacg
    93361 ataacaaaac cgagaattac agggggaata taaagccgga atttaatcat ttttattttc
    93421 ctggtgattc ggatgatgcg tcatacatcg cgcataaaca ctcatcaatt atccagtcat
    93481 cttcccagac ctgatattct gtcattggtt cgggctgttt tagcgggttc tcaatcgctt
    93541 gccgcgctct ttctttcagt aaaggatgat tatttctagc ccaggcattg atttgggtgg
    93601 taatggcgtt atcgatatcg tcctggttat ctctgatata atcctcacaa tatctgttca
    93661 ataaacaggc aagagaaacg gtcgaccaaa agccgggaac tgaattaatg gcatccagca
    93721 atggcgcaag agcgatatcg gcacggaaaa acatttccag catatactgt aattcgacag
    93781 cttcatcgcc ggatacccaa tcagtcatat actggcaggc aaatcgctgt aaaattgccc
    93841 gttcatccgc acgccagttt tctttgttgg caaggttgat acggcgcaga gaaaaaacta
    93901 cgtgaatatt gtcaaaatgc ccctgtgcaa caaattccag taaacgcggc agaaaatagc
    93961 ggacctcatc gctattttgc ggatcaggcc ctgggctact attccaggca ttgattaagg
    94021 aatacgggat cgctcgtaac gatgtactcc gtaatgcctt ttgttcctgc tgactcaggc
    94081 aatattcaca acaaaccaca aatttctgcg gcacccgata acgacgaaag gtgtgataaa
    94141 tctgctcaat taatgcctgc atggcggggc tgggttgcat agttaccccc tcgcccgctg
    94201 atcgcgtgta aacggttgat agacaaaacg agcattcgcc gcgttcaggc gaataagggc
    94261 gctacgcgaa agggaaaagc tggggcgaat gcaactattg cagtctcgac agctttcctc
    94321 tgcgcaattt tccactgagt aatgacgcgt gtagagtttc agttcggcct gcttatcgcg
    94381 aaatccgcgt aaactgttat tgttatgaaa tttccaggca tctttaaaga cctgaaagat
    94441 atagacatcc tcaatccagg agtcacattt tttaagcggg cgctttgcta ttatcacccg
    94501 ctcatccgga tttagcatcg ccgtaaattg tacggtgacg tcatttgtcg tgttcaggtc
    94561 acattgaaca tctggtacat cactgatata ggggtcaaat ccaaaatcag caccacaggc
    94621 acaaagacgg ctaatggtgg cggtatcgag ggaaaatccc ggccatacct tgctggaggt
    94681 taagccaata tttaatgtga catcatatcg aagcaatccc tcaagaccgt cagcaccgac
    94741 gaacttttcc ccttcatcca gggcctggtt aataagttgc tgaacaatgc cttcatccca
    94801 gtatgccgat ttgtagttgg caatcgtaat atccacgcct gtgaagggat cagtaccggc
    94861 aaggcttgca aataatgtat taactaattc catttttatt atccagcttt aaggtagcgt
    94921 cttatacatc gggacataac ctacatcttc caccaaactc tgtccctgcg gcgttaaaaa
    94981 ccactccaac aatttttgtg tctctgacgt cgtattttcc cgcgttatca taaaggcatc
    95041 gacgatatag ggatatttgc cgttgcgaat gttttcggca gtcggtgcaa taccgttaat
    95101 cgctagcagt tttatatttt tatcggcgtt catttgtgtt gcgtaataac ggaaggtgta
    95161 acctattgcg ttgtttgtat tacggtattc ggcaacgact ttaatcattc cctccatcat
    95221 acttgccact tctgtttcct gcggcgagat catgcggaca tttttcatga cctgggattg
    95281 catcaccgtc tggctaccag agtcttccgg gcgttgccag gtctggatct cctgatcgtt
    95341 accgccaaca gtacgccagt tggtaattgc accactgaag atgtcacgca cctgttgttc
    95401 agtcagggaa ttaaccggat tatccgcatt gacgatgaaa acaaacgctt cacgggcaaa
    95461 aggcgtgtac agcaaagtaa cgcccgattc ctcagcgcgt tttttctgcc caccggaagg
    95521 ttgcgccaca aaaataatat cagcatcacc tttaacaatc ctgttatacg catcgggggt
    95581 acgagagctc tccagatatt ccctggtgtg aaaatcttcc ggtataacgc ttaaagcata
    95641 aaatgcagaa gcataaattg ggtacgccgc cgtagcacca tcgagtcgcg gccagttttg
    95701 cgtaaactga atttgcggtt taccacgcag tggtgtcagt tgattattga gtttatcggg
    95761 tcgccaggcc caggtatcaa tattttcgcg cacaccgacg ccgggattga gctgtttata
    95821 taaataaccc tgataaccag cgacaaaacc aagcatgaga aggataaaca ggagggaatt
    95881 acggcaacgt agcccggatg taccggttaa aggatgtctg gttggccggc cgtaccccag
    95941 cgaaaaactg atatgcgcgc caacgggtat aatgactatc caccagtact cacctccgac
    96001 tactgagact aacaaaacgc ccagccaggg gagcataaga tcagccaacc aacccgatcc
    96061 catctggtga aattgataat ctgccagatg cagcgcgatt atccagccaa ccaggattaa
    96121 taaaccgggt actaaaaaaa gacattgtcg ccagacggct atttctcttt tttctggtgc
    96181 actatcgcct gcccatactc gccccgagaa aaatagcatt aatgcgccca gcgccagcat
    96241 aaaaatgttg agcagaaggt aacttgttgg cgtgataagg atattacaaa cttcaataat
    96301 accggcaagt ccgacaccca gcatagcaac aaccgccgcc aaaaattgcg tcagtatggg
    96361 gatgccgaaa aaagtcatta ccagcgagga caaaatccat tttctgtttt gcattattct
    96421 ttccattctt tttgaatggt gaaattatac tccccgagtc cccttgcccc ttctggacac
    96481 ttttccgaaa tgatggcgga aaaaaacggg agcctttggc cccgttctat ttattggtga
    96541 acttacaatc tcaccggatc gatatgccag atatgatcgg cgtactcttt gatagtacgg
    96601 tcagaagaga agtagcccat attggcaatg ttcagcatcg cttttgcggt ccactcttcc
    96661 tgaagctcgt agagttcgtc gactttatcc tgacaatcga catagctgcg ataatccgcc
    96721 agaacctgat agtgatcgcc gaagttgatc agcgaatcaa ccagatcgcg atagcgaccc
    96781 ggatcttccg gactgaatac accgctgccg atttgcgtca gcacctgatg cagctcctca
    96841 tctttctcgt agtattcacg cggtttgtag ccctgacgac gcagttcttc cacttcttcc
    96901 gcagtgttac caaagataaa gatattgtca gcaccgacat gatccagcat ctcgacgttc
    96961 gcaccgtcca gcgtaccgat agtcagcgcg ccgttaagcg caaacttcat gttactggtg
    97021 ccggaagctt ccgtccctgc cagcgaaatc tgttcagaca gatctgccgc cggaatgatc
    97081 aactgcgcca ggctaacgct gtagttcggg atgaacacga ctttcagctt atcgccaatc
    97141 tgcggatcgt tgttgatcac tttcgctacg tcattgatca aatgaataat gtgcttcgcc
    97201 atgtaatagg ccgaagccgc cttaccgcca aaaatattca cgcgcggtac ccacttcgca
    97261 tccgggtcgg ccttgatgcg gttatagcgg gtaatcacat gcaacacatt catcaattga
    97321 cgtttgtatt cgtgaatacg tttgatttgt acatcgaaca acgcctttgg attcaccacc
    97381 acattcagct gctgggcgat atactctgcc agacgctttt tgttctccag cttcgcctga
    97441 tgcacagcgt gattaaccat tgggaaatca cagtgttgtt gcagctcatt aagcaggcta
    97501 aggtcggtgc gccagttacg gcccaggtgt tcgtccagca cggctgaaag cgatgggttc
    97561 gctaccgcta gccagcgacg cggcgtcaca ccgttggtga cgttggtgaa acgacccggg
    97621 aagattttcg caaagtcggc aaacaacgat tgcaccatca gattagagtg cagttccgat
    97681 acaccgttaa ctttgtggct cacaacaacc gccagccagg ccatacgcac acgacgaccg
    97741 ttggattcat caatgatcga cgcccgtccc agcagatcgg tatcgttcgg atactgttcc
    97801 tgcaaggttt tcaggaaata gtcgttgatt tcaaagatga tctgcaggtg acgcggcaga
    97861 attttaccca gcatatcaac cggccaggtt tccagcgcct cgctcatcag cgtatggtta
    97921 gtgtaggaga agacctgaca acacacctca aacgcgtcgt cccagctaaa ttggtgctca
    97981 tcgatcagca gacgcatcat ctcaggaatc gacagtaccg gatgggtatc attgagatga
    98041 atcgcgattt tatccgccag gttatcgtag gttttatgca actgataatg gcggcttaaa
    98101 atgtcctgaa tggtcgagga aaccaggaag tattcctgac gcaggcgcag ctcacgcccg
    98161 gagtaggtgg agtcatccgg atacagtacg cgagatacgt tctcggagtg gtttttatct
    98221 tccactgccg cgaagtagtc accctggttg aatttaccga ggttaatttc gctactggct
    98281 tgcgcactcc acaaacgcaa cgtgttggtc gcgtcggtgt cgtaaccagg gattatctga
    98341 tcgtaagcga ctcccagaat ctcttcggtt tcaatccagc gcgttttttt accttcctgc
    98401 tgaatgcgac caccaaaacg gactttatag cgcgtgttgt ggcgtttgaa ttcccacggg
    98461 ttaccgtatt ccagccagta gtctggcgac tctttctggc taccgttaac gatgttctgc
    98521 ttgaacatac cgtagtcata gcggatgccg taaccgcgcc ccggcaaccc taacgtcgcc
    98581 agagaatcaa ggaagcaagc cgccagacgt cccaggccac cgttaccgag gcctgggtca
    98641 ttttcttcat caatcagctc ttcgagattt aaccccatcg cttccagtgc gccctgtaca
    98701 tcttcgtaaa ttcctagcga caacatggcg ttggagagcg tacggccaat caaaaactcc
    98761 atcgacaggt agtaaacctg acgagtttct tgcgacaact gggcacggtt tgaacgtaac
    98821 cagcgctcca cgagacgatc gcgcacagca aataacgttg cgttcagcca ttcatgttta
    98881 ttggcgacga ccgggtcctt tccaatcgta aacatcagct tgtaagcgat agagtgctta
    98941 agagcttcta cgctaagcgt gggcgatgaa tatgtaaacg gagcattcat ataggcgttt
    99001 cctgaaaact atttcaagcg atagtaaagc tcacggtacg acttcgccgc gacctgccag
    99061 ctaaaatcca ttgccatagc ctgacgttgc acaaaccgcc acagtgaagg acgggaccac
    99121 agtacaaaag cacgtcgaat agcccgtaac agcgaccagg cattactatc ttcaaagaca
    99181 aacccactgg cgacgccatc tgcaaggttc tcaagagaac agtcagaaac cgtatcagca
    99241 agcccaccgg tgcgccgcac taacggcagc gtaccgtact tcaatccata aagttgcgtt
    99301 aagccgcacg gttcaaaacg gctgggcacc agaatgacgt ccgcgccgcc cataatgcga
    99361 tgcgaaaatg cttcgtgata gccaatctga acgcccacct ggccggggta ttccgctgcc
    99421 gccgcaagga aaccttcctg cagcaccgga tcgcccgcgc cgagtagcgc cagctgcccg
    99481 ccctgctcca gaaggcccgg taaggcttcc agcaccaggt cgagaccttt ctggctggtc
    99541 agacggctca ccactgcaaa aagcggcact ttatcatcaa ccttaagccc cattgcgatt
    99601 tgtaactggc gcttattttc cgctttatct tccaacgtat cgcgggtgta gcgcgaggcc
    99661 aacagtaagt ccgtctctgg actccagatt ttctcgtcca cgccgttcag tacgccggaa
    99721 agacgccctt cacggtgacg ctgttgcaac agaccttcca taccgtaggc aaactgcggt
    99781 tcggtgatct cgcgagcgta ggttggacta accgccgtaa tgtgatcggc atagtacaga
    99841 ccggccttca ggaaagagat ttgtccgttg aattccagcc catgaatatt aaagaatgac
    99901 catggcaatt ggatgtcatt catatgatgt gcataaaaca tgccttgata ggccaggttg
    99961 tgcacagtaa acaccgactt cgccggacgc ccgcgcgccg ccagatacgc aggcgcaagg
   100021 cctgcatgcc agtcgtgcgc atgcaccaca tcaggacgcc agaatgggtc aagcccgctg
   100081 gccatttctg ccccaaccca ccccagcagc gcaaaacgca atacgttgtc ggtataggca
   100141 aataagttgg tatcgtgata cgggcttccc ggacgatcat agagatgcgg cgcgtcaatc
   100201 aggtatatgc caaccccgtt gtaatgaccg aacaacagcg tgatatgtcc ggcgaaggta
   100261 tcacgacggg atactacctg cgcatcggta acgccacggc gaatatcggg aaatgcaggc
   100321 aacagtacgc gagcgtcaac gccgtctgcg atttgtgctg cgggtaatgc cccaataaca
   100381 tcagccagac cgccggtttt aagcagcggg aacatctctg aacatacatg taaaacctgc
   100441 attatcgctc ctgtttatgc cctaacttcc gtagcatttc gcgcgttacc agcacgatgc
   100501 cttcttctga acgatagaaa cgacgtgcat cttcctctgc gttttcacca atcaccatgc
   100561 cttccggaat aacacaagca cgatcgatga cgcagcggcg cagacggcac gagcgaccta
   100621 cccatacttc cggtaacaat acggcggaat caatgttgca gaatgaattc acgcgaacgc
   100681 gcgagaacag aacggactgc accaccaccg aaccggagat cacacagccg ccggatacca
   100741 gtgagttaag ggtcatcccg tggctaccgg agcgatcctg cacgaatttc gctggcggta
   100801 atgattcatt gtaggtgcga attggccaat tgcgatcgta catatccagt tccggcacca
   100861 cagaggccag atcgaggttc gctttccagt aagcttccag cgtacccaca tcgcgccagt
   100921 acggctcggc atccgggtcg gattgtacgc aagagagcgg gaacgggtgc gcataggcca
   100981 gaccggcttc ggtgatcttg ggaatcaaat ctttgccaaa gtcgtggctg gagttctcat
   101041 cacgatcgtc ttcttccagc agttcataca gatagtcggc gtcaaagacg tagataccca
   101101 tactcgccag agatttgctc ggatcgttcg gcattgacgg tgggttagca ggtttttcaa
   101161 cgaattcgat aattttatcg ttctcatcaa ccgccataac gccaaatgcg gaggcttctt
   101221 caatcggtac tggcatacaa gcaacggtgc aacgtgcgcc tttttcgacg tgatcgataa
   101281 gcatacgcga gtagtcttgc ttgtagatat ggtcgcccgc caggatcacc acgtattccg
   101341 ctttataacg gcggataatg tcgaggtttt gggtgaccgc atctgcggtg ccgcgatacc
   101401 agttttcccc tttcattctc tgctgtgctg gcagcagatc gacaaactcg ttcatttctt
   101461 cattgaagaa tgaccagccg cgctgaatgt gctgcaccag agtgtgggac tggtactggg
   101521 tgatcacgcc catacgacgg atcccggagt tgatgcagtt agacagcgca aagtcgataa
   101581 tgcggaactt accgccgaag tgtacggccg gttttgctcg cttattggtt aaatccttca
   101641 ggcgggtacc acgtcctccc gccagtatca gggcaacaga tttcaatggc agctggcgcg
   101701 ccaacattaa gtgatcgttc ttctctaaac taaccatgac taactccttt tttatcatct
   101761 ctggaacaca cacaatccgt gtgcaggtcc ctgccagaca gccgtaatca ctgggttatc
   101821 ctctccagcg aatgggggaa tggcgtgcca ctccccagca ggtaaaacaa tctctgttac
   101881 ctcaagcgtg gcgttaattg cgatcaaaaa gcgatccgag agcagaattt gcagctgttt
   101941 cggcccgttt tgccactcat ccgtgcttaa aggttgagca tatcgattta gccaacggac
   102001 attgccgtcg ccttcttccc accagcgatt ctccaccaaa gcgggaatgc gcttgcgcag
   102061 atggattaac gcggcggtaa atgcggttaa accactgctt gcctgcgacc agtccaacca
   102121 ggttaattgg ttatcctgac agtaggcatt gttattgcca tgctggctgt gaccatgttc
   102181 gtcaccggcc agtaacatcg gcgtaccctg ggagagcaac aacgttgtta acagggcgtg
   102241 aatgctgtcg cgccgccgtt caaccaggtc aagagaaccg cctaaccctt ctttaccatg
   102301 attgttactg taattgttgt tggtcccgtc gcgattttct tctccgtttg cttcattgtg
   102361 tttatggttg aagcaaacgc agtcgcgaag cgtaaaaccg tcatgcgcgg tgacgagatt
   102421 aatcgcggca ctcggcagac gaccattacg tttaaaaaca tcgctggagg cagcaaaacg
   102481 cccggcaaac gcccccagag gcaaatcata atgtagccag aaacgacggg cagcatcgcg
   102541 gaaatgatcg ttccactcgg caaacagcgg cgggaaattt cccacctgat aaccaccagg
   102601 agcgatatcc cacggttcag caattaactt cacctgcgag agcaccgggc agttctggat
   102661 agcggtaaac aacggcgcat cctgacggaa ctctggcgta cggcccatga ctgccgccag
   102721 atcaaagcgg aaaccatcga cgtggcaggt ttctacccaa taacgcaggc aggcgctggc
   102781 ataatccacc accgccggat gactcaaatt gagcgtgttg ccgcaaccgg tccagttgtg
   102841 ataatcgccg tcttctctta tccaataata gctacggtta tcgatcccac gcagcgagaa
   102901 taacgggccg tcgaggtcca gttccgcact atggttgagc acgatatcaa gaatgacttc
   102961 gatacccgct ttatgcagtg ctttgattgc atcgcgaaac tcatccagcg ccgtttctgg
   103021 cgagcaggca tacgccggat gcagcgcaaa catcgccacc gggttgtaac cccagtagtt
   103081 acttagcccc atgcgttgca gacgtggttc actggcaaac tgcgccactg gcagcagttc
   103141 cagcgcggta atgcccaatt gtttcaaata gttgatcatc accggatgcc cgagggcttt
   103201 ataagtgcca cggatctcga ccgggatctc cgggtgcaag tacgttaatc ctttgacatg
   103261 ggcttcataa atgatggtgc tgccccacgg cgtgcgcggc ggggcatcat cttcccagtc
   103321 atagtgatca accaccacta cgcatttcgg cgcaatggcg gcgttgtcgc gatagtcagg
   103381 ttcattatga ccggcgtgca gcagcgggtt atctttaaac tccccgtcaa tttgccgcgc
   103441 gcaaggatca atcaacaact tcgccgggtt aaagcgatgc ccctcggcgg gttgccaggg
   103501 gccatgaacg cgataaccat aacgcaaacc cgggcgcgca tccggcagat aaccgtgcca
   103561 aatgtcgcca ctgtgccctg gcaagtcata gcgatgttcc tggccattgg cgtcaaagac
   103621 acacagttct acccgctcgg catgagcgga gaaaagtgtg aagttgacgc cctgaccgtc
   103681 gtaatgcgcg ccgaggggag cgggtttgcc aatggcgagt tgtgtcattc tgcctcccga
   103741 accagccaga tagtggccag cggtggtagc gtcaggctta gtgaatgctg acgaccgtgg
   103801 ctggcaatct catcgctgtg taccgtgccg ccattgcctg cattactgcc gtgatagtgc
   103861 atggaatcgg tattgaggat ttcacgccat ttgcccggct ggtttatgcc gaagcgataa
   103921 tcatgacgcg gtaccggcgt aaagttactg gcaacgatga tttcgttacc ctctttatcg
   103981 cgacgcacaa agatcagcac cgagcgttct ttgtcatcca ccaccagcca ttcaaagccg
   104041 tacgggtcaa aatccagttc atgcattgct ttatggtggc ggtaggtgag gttcagatcg
   104101 cgcaccagac gctggacacc gtggtgccag ttatcgccgc cttccaacag atgccagtcg
   104161 aggctggcgt catggttcca ctcgcggccc tgggcaaatt cgttacccat gaacagtagt
   104221 ttcttgcccg ggaatgccca catccagcca tagtaggcgc gcaggttcgc gaatttctgc
   104281 catgcgtcgc ccggcatgcg gtcgagaatc gattttttac cgtggaccac ttcatcatgc
   104341 gacaacggca ggacgaagtt ttcagtgtag ttgtagagaa tcccgaaggt cagtttatcg
   104401 tgatgatact gacgataaac cgggtcgagc ttcatgtagt ccagggtgtc atgcatccag
   104461 ccgaggttcc acttgtacca gaagcccaga ccgcccatat cctgcggacg agaaacgcca
   104521 gggaaatcgg tagactcctc agccattgtc accgcaccgg aaacctgctc accaagaata
   104581 cggttggtat tacgcaagaa ttcaatcgct tcaagattct cgcgcccgcc aaattcgttc
   104641 gggatccact ccccctcttt acggctgtag tcgcgataaa tcattgacgc caccgcatcg
   104701 acgcgcagcg catcaatacc aaaacgttca atccagtaaa gcgcgttacc gacgaggaag
   104761 ttactgactt cacggcgacc atagttgtag atcagcgtgt tccagtcctg atgatagcct
   104821 tcacgcggat cgctgtgttc atacaagttc gtgccatcaa attcggcaag cgcaaagtca
   104881 tcagtcggga agtggcctgg cacccagtcg agaatcacgt tcagaccagc tgcgtgtgcg
   104941 gcatcaatga aataacggaa gtcgtcgcga gtaccaaaac ggcgggttgg tgcatacagg
   105001 ccggttggct gataacccca actgccatcg aagggatgct cgttaatggg cagtagttcg
   105061 aggtgggtaa agcccatcca tttagcataa ggcaccagtt gatcggccag ctcgcggtag
   105121 ctcaaccaga aattgttgtc ggtgtggcga cgccaggaac ccaggtgaac ttcataaata
   105181 gagattggcg catcaaactg attcgctttt ttgcgctctt cagtctgtac aaccttttcc
   105241 ggcagcccgc aaataagaga cgcggtttcc gggcgcattt gcgcttcgaa ggcataaggg
   105301 tcggacttca gacgcaagtt gccattggca tcaatcatct cgtatttata gagctgaccg
   105361 ttatgcgccc cagggataaa cagttcccag atgccgctct ctttacgcag gcgcatcggg
   105421 tgacggcgac cgtcccagta gttgaattgc ccaaccaccg agacccgacg ggcgtttgga
   105481 gcccagacag agaaacgcgt acctgtgacg ccatccatag tatctgcatg cgcgcctaag
   105541 gtttcatacg ggcgcaggtg agtaccttca gataatagcc aggcatccat ttcctggatt
   105601 agcggaccaa aacggtaagg atcatcaatc aggttttgct gaccatgcca gacaacagcc
   105661 aactgatagc ggaaaaaatt cttacgtcgc ggaatgacgc cgctaaagaa tccccgtgag
   105721 tcgagacact ccagttttgc gagtttgcgc ccggttttcg gttcaatcac ccacacatcg
   105781 gtagcgtcgg gtaaaagggc acggacttcc agtcccgcgg tggttttatg cattcccagt
   105841 acggaaaaag gatccgcaaa atggcctgca attagcgcgt taatcacgtc tctatcgata
   105901 cgatcggaca tgcttgtctt cctgttttat tgtgtcaccc catccaattg gatttttgac
   105961 ttctggttgt gacatttttt tgacctgaac ggcgcagcac tctgtgcatc ctctctgcgt
   106021 cgtcctcact tcaggtaagg ctgtgaatac tcatgtattc agccaccctt aaagaatagc
   106081 caatgctcta tttaactccc ggtaaatcat gaaacatctg cgcttactcc tgtattacgc
   106141 actaacaggg gcggcatcgc gccccagatt taatgaataa agattacgcc agttgacgaa
   106201 gcatccgacg cagcggctcc gcggcccccc acagcagctg gtcgcccacg gtaaaggctg
   106261 acaggaactc tggtcccata ttcagcttac gcaggcggcc taccggcgtg gtcagcgtgc
   106321 cggtaacggc agctggggtt agctcacgca tagtgatttc ccgatcgttc ggaacgactt
   106381 tcgcccacgg attgtgcgca gccagcagtt cttccacggt cggaatagac acatcttttt
   106441 tcaatttaat agtgaatgcc tggctgtggc agcgcaatgc cccgacacgc acacataaac
   106501 catctaccgg aattacggaa gatgtgttga ggatcttgtt ggtttccgcc tgccctttcc
   106561 actcttcgcg gctctgaccg ttatcgagct gtttgtcgat ccacggaatc aggctacccg
   106621 ccagcggcac gccaaagtta tccaccggca gctcaccgct acgggttaag gttgtgactt
   106681 tgcgttcgat atcgagaata gcagaggacg gggtcgcgag ttcatctgcc acatggccat
   106741 acagatggcc catctgggtt aataactcac gcatatgtcg cgcaccaccg ccggaagcgg
   106801 cctggtaggt tgcaacggac acccaatcaa caagatcatt ggcgaataaa ccacccaacg
   106861 acatcaacat caggcttacg gtacagttac cgccaacaaa agtcctgatg ccattattta
   106921 atccgtcggt aatgacgtcc tgattgacgg ggtcaagaat gatgatggcg tcatctttca
   106981 tgcgcagaga cgatgctgcg tcaatccagt aaccttgcca tccgctttca cgaagctttg
   107041 gatagatttc gttggtataa tcgccgccct gacaggtcac aatgatatcg aggaccttta
   107101 gcgcctccag atcaaaggca tcctgaagtg tgccagtggt tccgccaaaa gacggcgcag
   107161 cctggccaag ctgagaagta gaaaagaaga cagggcgaat ggcgtcgaag tcgcgctctt
   107221 caaccatgcg ttgcatgaga acggagccga ccataccgcg ccagccgata aaaccaacat
   107281 ttttcataag cgttttttcc tgcaaagatg tgtgctgtat aaatgtgccg gtctcctctt
   107341 ggcacatctt tcaccataca aaaagcagcc aaagtcgcaa gtgaaattaa tcaatgatag
   107401 cgaagccatc agtaatgcga cttatcctgc tttgttagca cgcagaaagt ccgcggcaat
   107461 tatcagggaa tttgagttat gaatgaaatc atttctgcag cagttttatt gatcctgatt
   107521 atggatccgc tcggaaacct acctattttc atgtccgtac tgaaacatac tgaaccgaaa
   107581 agacggcggg caatcatggt gcgagagttg cttattgctc tcctggtgat gctggtgttc
   107641 ctgtttgcgg gtgagaaaat tctggcattt ctcagcctgc gagcagaaac cgtctccatt
   107701 tctggcggca tcattctgtt tctaatcgcc attaagatga ttttccccag cgcttcagga
   107761 aatagcagcg ggcttccggc aggtgaagag ccatttatcg tgccgttggc tattccgtta
   107821 gtcgccgggc cgactattct cgccacgctg atgttgctgt ctcaccagta cccaaatcag
   107881 atgggacatc tggtgattgc tctgctgctg gcctggggcg gcacctttgt catcctgcta
   107941 cagtcttcgc tatttttacg tctgctgggc gagaaagggg tgaacgcact tgaacgcctg
   108001 atgggattga ttctggtgat gatggcaacc cagatgttcc tcgacggcat tcgaatgtgg
   108061 atgaaggggt aacgaggaga gtttttcgaa aagaacccgg tagcgtcaca actaccgggc
   108121 gaacaaactt aactcaacag ctggaacgca atcatcccaa cgatggcacc gacagtgccg
   108181 aggatggttt ccatcatggt ccaggttttc agcgtttcgg cttcggtcgc gccggtaaat
   108241 ttaccgaaca accagaaacc ggcgtcgtta acgtggctga caacaatcga accaccagcg
   108301 atacaaatcg acagcgccgc catttgcgca ccggagtagt tcagttgttc aataaccggc
   108361 atcaccagtc ctaccgccgt taaacaggct acggtggcag aaccctgaat gatgcgcact
   108421 gcagcggcca gcacgaagca ggtgatggct atcggcaggc ccatgccagt taacgcttcg
   108481 cccagtgccg gacctacgcc agagtcaacc agcacctgtt tgaacacgcc acctgcacca
   108541 atcaccagca gaatgatccc cgccggttgc agcgcgtgac cgcaaatctc catcactttg
   108601 tcttttggca tgccctgacg cattgccagg ccataaatcg ctaccagaca agcaaccaga
   108661 atcgcggtaa acggatgacc aataaactcg aaccattcgt aagcggtaga gccttctggc
   108721 acaaaacgcg cggcaatggt tttcagcccc accagcacca gcggcaacag gatcagcgac
   108781 aggctgaatc cgaaagatgg cattttgcct tcgccgagat gcggttcgct gatgtcgtca
   108841 ggaatatgca actccacgta gcggctgatg aaattacccc acagcggccc ggcaataatc
   108901 attcccggaa ttgccgcaca caggccaatc aggatcatcc agccaaaatc ggcgttcatc
   108961 tgcgatgcca gcagcattgg cgctggccct ggcaccagga acgccgccgc tgccgccacg
   109021 cctgcgaata atgggattac cagcttcacc aggttcgtac cggtgtggcg cgccattgag
   109081 aaagcaacgc taatcagcag aacaatcgcc acttcaaaga acagcggtag cgcacagacc
   109141 agccccgcaa ggccgatggc ataatgcgcg cggctgtgac cgaaggattt aagcattttg
   109201 acggcaatct gatcgactgc gccggtttca tgtaagatct tgccaaacat agctcccagg
   109261 gcgacaacca ccgccaggaa gccgagggtg cctcccatcc ctttttccat cgtcgctgcg
   109321 attttatcga gcggcatacc ggaaaaaagg ccagccccca tggacaccac catcaaagcc
   109381 aggaaagcgt gcatacgcgc cttcatgact aaaaacagca gcagtaaaac agaccctact
   109441 gctgttaaaa caagcgttaa tgtagtcact acttatttgc cttttttaat aacctcaatg
   109501 gtgcttgcca caacaccttc cagcggttga tcgatatcca ccaccagtac atcagtttcg
   109561 tccgcacccg gctcctgcag cgtttcaaac tgcgtcacca acatttgggt tttaaagaaa
   109621 tggcctttgc gcgctttcag gcggctttca atcacgtcaa aatcgccttt caggtagatg
   109681 aaagagagat tcggattacc ttcacgcagc aagtcgcgat agtgtttttt caatgcagaa
   109741 cagacgatca gtgacacttt attagtgcgc tgcatagcaa acgcggcgtc gttcagcgcc
   109801 tgcaaccacg gtttgcgatc gtcgtcattc agcggttcgc cagacgccat tttttcgata
   109861 ttgcgccgtg gatggaggaa atcgccatca agaaacgcgg catgaagttg atgcgccact
   109921 tcactggcga ccgcagattt gccgctgccc gatacgccca tcaagacgta aatgtggtga
   109981 tcatggttag tcgtgctcaa agtggtgccc ccacaataca agaattaaaa atgttacggg
   110041 taactgttat cggtaacatt gtccagccgg acaatatcag aagcaatatc catccgtgcc
   110101 ttaagtgtat aagtgtgagc tacttcaaat ttgtaggctt aaatagatcc gcccggtgac
   110161 aaggtgaaac ctaaatctaa cattttcggt gtcacagatt cgccacgaat acgcgccagc
   110221 aggcgttcag cgccaatact gcccatccgc tcacgcggcg tcagcacgct cgctaaccgc
   110281 ggctccatca cctggccaat gtcatgaccg tggaaaccgg caatcgccat atcgtcagga
   110341 accttcaatc ccagacgctg acattcaaac gccgcgccga ccgccaggtc atcgttggta
   110401 cagaacacgc catccagctg cggatattcc cgccgcgcct ggcgaatcag ttcaataccg
   110461 gaagagtaag aagaagattg ctcaaccatc acgctatacg gcaccagacc tgcatccagc
   110521 atcgcctgtt cgtatccctt ctgtttgatg atagtacgtt cgtcgagacg cgcgccgaga
   110581 taggcaatgt ggcaatgccc gcgagcaata atggcggtgg tcatctggcg tgccgcttca
   110641 aagttatcaa aaccgacggc gatatcgagg cacggcgact ggctgtccat gagttccacc
   110701 accgggatcc ccgccacttc aatcatcttt aaggtgcgcg gcgtgtgggt acgttcggtg
   110761 aggatcagtc cgtcgatatt ccatgagagc atggattcga gacgttcttg ctccatttcc
   110821 ggtttataac cgtagtgcgc cagcatggtc tgataaccgt gcgcgtcggt gacgctttcg
   110881 attccgcgta atacttccgc gaaaacctgg ttggtgagag aaggtaacag gacgccaatc
   110941 gcccggctgg tggcgttaga gaggatatcg ggcgcacgat tgggaatata gcccagttca
   111001 tcaagagcgg cggcaatctt gccgcgtaga gcgacggaaa cctgctccgg gttgcgtaaa
   111061 aaacggctga ccgtcatttt ggtcacgcct acacggtcag ccacatcctg aagtacgggt
   111121 cttttctttt tcatcgtcct gaaggtacaa aagagataga ttctcttagt ttaacacgga
   111181 cgcatcagaa ccttcccggg gaaaacagtg cttgtttaca attattagat atacactaaa
   111241 tcaattgcat cgcattgtgc taacgacgcg gcaaaacccc ggaagcgtgc atcagcacgc
   111301 gaccggagcg agcgaggaag cctgcaaaaa ggcaggcttc aaaaatgccg tggagcttta
   111361 aaccggcggc agatcgaaca gtaacacttc gctgtcgcta tccgcatgga tggagattgc
   111421 ctgctcatcc cagattgcca gaccatcgct ggtcgaggct ttcacgccgt taatggtgac
   111481 attgcctttc accacctgga tccagacgcg gcgttcagcg gcaatctgat gcaccgactg
   111541 ctcatctttc agcaacgccc agcggtacag ttccatatcc tgatgcactt tcaatgaacc
   111601 atctcgcgca tccggcgaga gcaccagctg tttgccctgt acggcatcga agcgacgctg
   111661 ttcataacgc ggcgtaatac cgttttcttc aggcatgatc cagatctgat acagatgcag
   111721 acgctcggtg ctgcttgggt tgtactctga gtgacgaata cccgtacccg cactcataat
   111781 ctggaactca cccgccggaa cctgctcttt attgcccatg ctgtcctgat gctcaacagt
   111841 accttccagc acgtaggtca aaatttccat atctttatgc gggtgagtgc cgaagccctg
   111901 ccctgcttca atcacgtcgt cgttaatcac gcgcagcgcg gagaagccca taaagttcgg
   111961 atcgtaatag ttggcaaaag agaaagtatg ccaggagtcc agccagccat gatttgcatg
   112021 accacgttca tttgctttgc gtaagtagat catttgtatt cgccccctga atgatttcga
   112081 tgggcttagt gtggacccga tctgcctggg atgatagagg gtgaaaattg acccctctgt
   112141 tcaaaaaaat tgaactattc gaggggccat caaacttaat tcgcgagagt cactgtggag
   112201 ggagaggctt gctcaaatcc gcgttcaagg atttccagat tggtaagaac ttcagattcc
   112261 ttgacgtaat ttggcgcacc gtgggtgatg gtttgataca acgcatcata aacgcgcccg
   112321 taatcgccca tctccggctt catctcttct ctgaccgtca cgccctcgtc attgacatac
   112381 tccagcacac cgaccgaatt atccgctgcg aatcccggtt cgcccggcat aatattagcc
   112441 ttcaggctgg tttcctgctg gtcgataccg tatttaataa acgaaccttt cttaccgtga
   112501 acgataaatt tcggataatc gattttcacc agatggctgg ttttgacgat ggctttcagg
   112561 tcgccataaa acagttgcgc ttcaaaggtg tcgtcaggat tggctttatt acgcaggctg
   112621 cggatgtcat aagcgacgtg atccgggcga ccgaacagag aaataatctg gtccatcgta
   112681 tgcacaccaa ggccataaaa cgcgccatcc tgcggcagcc caggtttggt ttctgccacc
   112741 gggcggtaat agtcaaaatg gctttccact tcaacaatct ctcccaactt gccactttca
   112801 atcgcttttt tcgctgtcag gaagcaggag tcaaagcgac gattctgata cggcgtgacg
   112861 gtcagccctt tgcttttcgc caacgcaaac agctctttcg cctgcgcaag tgtcggagtg
   112921 aacggttttt cgaccagcac atttttcccg gcttccagcg cgcgcttcgc gtactcgaag
   112981 tggctgtccg cgtgggtgca gacaacaacc agcttaacat cgggatcgtt tagtacttcg
   113041 tcgagatcgc tggtgaaatg gatatgggaa taaatgggag cctgttcttc cggcttcgca
   113101 tggcgacgaa aaatatgcgc gacatgccag ctatccttgc ggttaagtac atacggcaga
   113161 tggtaacggg tggtgctttt gccgaagcca ataaaggcgc agttgatgac catgatttcg
   113221 tcctttttaa ggtggttatt tacaccttag cgcaaagcgg acgtggttcc tactggagtg
   113281 cgcataacga acacaagcac tcccgtggat aaattgagaa cgaaagatca aaaaaaaagc
   113341 cagcacccgg ctggctaagt aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt
   113401 ctccgcgagg gtcttcctga tcgcgagaca ataataatca ttctcattcg cacttgtcca
   113461 acactttttg caaaaaaatg catttgactc gcatttgaaa gtcaatgatg ttgaaaggga
   113521 catttacccc aaagaggaca aaggaatgag tgagatagta atacgccacg cagaaacacg
   113581 ggattacgag gccatcaggc agattcacgc ccagccggag gtgtattgca acacactaca
   113641 ggtgcctcat ccttccgatc atatgtggca ggagcgactc gccgatcgtc ccggcatcaa
   113701 gcaactcgtc gcctgtattg atggagacgt cgtgggccat ctcaccattg acgtgcaaca
   113761 gcgcccacgc cgcagtcatg ttgccgattt tggtatctgt gtcgactctc gttggaagaa
   113821 ccgcggcgtc gccagcgccc tgatgcgaga gatgattgaa atgtgcgaca actggttgcg
   113881 ggtagatcgc attgaactaa ccgtgtttgt cgataacgcc ccggcaatta aggtctataa
   113941 aaaatacggc tttgaaattg aagggactgg taagaagtac gcattgcgta atggtgaata
   114001 tgtcgatgca tattatatgg cgcgggtgaa gtaagatagt gccctttttc tgagatggaa
   114061 aaagggtgtc attcaaaatc gacatacctt cctttaaggt atttatttgc ccaatacata
   114121 tattgagatt taactcataa ttacttccta aagtgtaata ttttattttt taatatatac
   114181 gcctacaatt tcctggagta aataaataac aattaacaag cataatattg ccattgataa
   114241 aatagcatgc cataaaagga cttttcaggg atgagtaata ttgtttacct gacagtaacg
   114301 ggagaacaac aaggaagcat ctccgcaggt tgtgggactt ctgagtctac aggtaatcgt
   114361 tggcagagcg ggcatgagga tgaaatattt acattctcac tcttaaataa tattaataat
   114421 acggggcttg gttcacagtt ccatggtata acattttgta aattaattga taaaagcact
   114481 ccattattta
//
LOCUS       DAZFJF010000002       100657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFJF010000002 DAZFJF010000000
VERSION     DAZFJF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.002"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHKAU_000106"
     CDS             complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHKAU_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO5529247.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHKAU_000107"
     CDS             complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHKAU_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO5529248.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4356..5549
                     /gene="acrA"
                     /locus_tag="ACHKAU_000108"
     CDS             4356..5549
                     /gene="acrA"
                     /locus_tag="ACHKAU_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO5529249.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5572..8721
                     /gene="acrB"
                     /locus_tag="ACHKAU_000109"
     CDS             5572..8721
                     /gene="acrB"
                     /locus_tag="ACHKAU_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO5529250.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            9267..9641
                     /gene="tomB"
                     /locus_tag="ACHKAU_000110"
     CDS             9267..9641
                     /gene="tomB"
                     /locus_tag="ACHKAU_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO5529251.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            9667..9885
                     /gene="hha"
                     /locus_tag="ACHKAU_000111"
     CDS             9667..9885
                     /gene="hha"
                     /locus_tag="ACHKAU_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO5529252.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            10057..10608
                     /gene="maa"
                     /locus_tag="ACHKAU_000112"
     CDS             10057..10608
                     /gene="maa"
                     /locus_tag="ACHKAU_000112"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO5529253.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            10724..11194
                     /gene="ylaC"
                     /locus_tag="ACHKAU_000113"
     CDS             10724..11194
                     /gene="ylaC"
                     /locus_tag="ACHKAU_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO5529254.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            11358..12908
                     /gene="pdeB"
                     /locus_tag="ACHKAU_000114"
     CDS             11358..12908
                     /gene="pdeB"
                     /locus_tag="ACHKAU_000114"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO5529255.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(12950..13303)
                     /gene="ybaA"
                     /locus_tag="ACHKAU_000115"
     CDS             complement(12950..13303)
                     /gene="ybaA"
                     /locus_tag="ACHKAU_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO5529256.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13423..13519)
                     /gene="ffs"
                     /locus_tag="ACHKAU_000116"
     ncRNA           complement(13423..13519)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKAU_000116"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            13681..13992
                     /locus_tag="ACHKAU_000117"
     CDS             13681..13992
                     /locus_tag="ACHKAU_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO5529257.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(14023..14595)
                     /gene="ybaY"
                     /locus_tag="ACHKAU_000118"
     CDS             complement(14023..14595)
                     /gene="ybaY"
                     /locus_tag="ACHKAU_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO5529258.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            14813..15673
                     /gene="tesB"
                     /locus_tag="ACHKAU_000119"
     CDS             14813..15673
                     /gene="tesB"
                     /locus_tag="ACHKAU_000119"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO5529259.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(15722..17008)
                     /gene="amtB"
                     /locus_tag="ACHKAU_000120"
     CDS             complement(15722..17008)
                     /gene="amtB"
                     /locus_tag="ACHKAU_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO5529260.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(17038..17376)
                     /gene="glnK"
                     /locus_tag="ACHKAU_000121"
     CDS             complement(17038..17376)
                     /gene="glnK"
                     /locus_tag="ACHKAU_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO5529261.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(17557..19338)
                     /gene="mdlB"
                     /locus_tag="ACHKAU_000122"
     CDS             complement(17557..19338)
                     /gene="mdlB"
                     /locus_tag="ACHKAU_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5529262.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDTDTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(19331..21103)
                     /gene="mdlA"
                     /locus_tag="ACHKAU_000123"
     CDS             complement(19331..21103)
                     /gene="mdlA"
                     /locus_tag="ACHKAU_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5529263.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(21133..21591)
                     /gene="decR"
                     /locus_tag="ACHKAU_000124"
     CDS             complement(21133..21591)
                     /gene="decR"
                     /locus_tag="ACHKAU_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO5529264.1"
                     /translation="MLDKIDHKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(21744..22562)
                     /gene="cof"
                     /locus_tag="ACHKAU_000125"
     CDS             complement(21744..22562)
                     /gene="cof"
                     /locus_tag="ACHKAU_000125"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO5529265.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            22662..24362
                     /gene="ybaE"
                     /locus_tag="ACHKAU_000126"
     CDS             22662..24362
                     /gene="ybaE"
                     /locus_tag="ACHKAU_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO5529266.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            24427..25122
                     /gene="queC"
                     /locus_tag="ACHKAU_000127"
     CDS             24427..25122
                     /gene="queC"
                     /locus_tag="ACHKAU_000127"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO5529267.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(25174..25572)
                     /gene="fadM"
                     /locus_tag="ACHKAU_000128"
     CDS             complement(25174..25572)
                     /gene="fadM"
                     /locus_tag="ACHKAU_000128"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO5529268.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(25666..26037)
                     /gene="ybaV"
                     /locus_tag="ACHKAU_000129"
     CDS             complement(25666..26037)
                     /gene="ybaV"
                     /locus_tag="ACHKAU_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO5529269.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(26188..28059)
                     /gene="ppiD"
                     /locus_tag="ACHKAU_000130"
     CDS             complement(26188..28059)
                     /gene="ppiD"
                     /locus_tag="ACHKAU_000130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5529270.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(28251..28541)
                     /gene="hupB"
                     /locus_tag="ACHKAU_000131"
     CDS             complement(28251..28541)
                     /gene="hupB"
                     /locus_tag="ACHKAU_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO5529271.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(28732..31086)
                     /gene="lon"
                     /locus_tag="ACHKAU_000132"
     CDS             complement(28732..31086)
                     /gene="lon"
                     /locus_tag="ACHKAU_000132"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO5529272.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(31274..32548)
                     /gene="clpX"
                     /locus_tag="ACHKAU_000133"
     CDS             complement(31274..32548)
                     /gene="clpX"
                     /locus_tag="ACHKAU_000133"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO5529273.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(32674..33297)
                     /gene="clpP"
                     /locus_tag="ACHKAU_000134"
     CDS             complement(32674..33297)
                     /gene="clpP"
                     /locus_tag="ACHKAU_000134"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO5529274.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(33544..34842)
                     /gene="tig"
                     /locus_tag="ACHKAU_000135"
     CDS             complement(33544..34842)
                     /gene="tig"
                     /locus_tag="ACHKAU_000135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO5529275.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKARIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(35186..35503)
                     /gene="bolA"
                     /locus_tag="ACHKAU_000136"
     CDS             complement(35186..35503)
                     /gene="bolA"
                     /locus_tag="ACHKAU_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO5529276.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            35808..36386
                     /gene="yajG"
                     /locus_tag="ACHKAU_000137"
     CDS             35808..36386
                     /gene="yajG"
                     /locus_tag="ACHKAU_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5529277.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            36430..37905
                     /gene="ampG"
                     /locus_tag="ACHKAU_000138"
     CDS             36430..37905
                     /gene="ampG"
                     /locus_tag="ACHKAU_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO5529278.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            38365..39312
                     /gene="cyoA"
                     /locus_tag="ACHKAU_000139"
     CDS             38365..39312
                     /gene="cyoA"
                     /locus_tag="ACHKAU_000139"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO5529279.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            39334..41325
                     /gene="cyoB"
                     /locus_tag="ACHKAU_000140"
     CDS             39334..41325
                     /gene="cyoB"
                     /locus_tag="ACHKAU_000140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO5529280.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            41315..41929
                     /gene="cyoC"
                     /locus_tag="ACHKAU_000141"
     CDS             41315..41929
                     /gene="cyoC"
                     /locus_tag="ACHKAU_000141"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO5529281.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            41929..42258
                     /gene="cyoD"
                     /locus_tag="ACHKAU_000142"
     CDS             41929..42258
                     /gene="cyoD"
                     /locus_tag="ACHKAU_000142"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO5529282.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            42270..43160
                     /gene="cyoE"
                     /locus_tag="ACHKAU_000143"
     CDS             42270..43160
                     /gene="cyoE"
                     /locus_tag="ACHKAU_000143"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO5529283.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            43345..43656
                     /locus_tag="ACHKAU_000144"
     CDS             43345..43656
                     /locus_tag="ACHKAU_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO5529284.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            43640..44182
                     /locus_tag="ACHKAU_000145"
     CDS             43640..44182
                     /locus_tag="ACHKAU_000145"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5529285.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(44238..45173)
                     /locus_tag="ACHKAU_000146"
     CDS             complement(44238..45173)
                     /locus_tag="ACHKAU_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5529286.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            45581..46945
                     /gene="yajR"
                     /locus_tag="ACHKAU_000147"
     CDS             45581..46945
                     /gene="yajR"
                     /locus_tag="ACHKAU_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5529287.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(47073..47564)
                     /gene="yajQ"
                     /locus_tag="ACHKAU_000148"
     CDS             complement(47073..47564)
                     /gene="yajQ"
                     /locus_tag="ACHKAU_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO5529288.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            47732..48643
                     /gene="panE"
                     /locus_tag="ACHKAU_000149"
     CDS             47732..48643
                     /gene="panE"
                     /locus_tag="ACHKAU_000149"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO5529289.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            48606..49196
                     /gene="yajL"
                     /locus_tag="ACHKAU_000150"
     CDS             48606..49196
                     /gene="yajL"
                     /locus_tag="ACHKAU_000150"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO5529290.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(49250..50698)
                     /gene="thiI"
                     /locus_tag="ACHKAU_000151"
     CDS             complement(49250..50698)
                     /gene="thiI"
                     /locus_tag="ACHKAU_000151"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO5529291.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            50904..51146
                     /gene="xseB"
                     /locus_tag="ACHKAU_000152"
     CDS             50904..51146
                     /gene="xseB"
                     /locus_tag="ACHKAU_000152"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO5529292.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            51146..52045
                     /gene="ispA"
                     /locus_tag="ACHKAU_000153"
     CDS             51146..52045
                     /gene="ispA"
                     /locus_tag="ACHKAU_000153"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO5529293.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            52070..53932
                     /gene="dxs"
                     /locus_tag="ACHKAU_000154"
     CDS             52070..53932
                     /gene="dxs"
                     /locus_tag="ACHKAU_000154"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO5529294.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            54112..55086
                     /gene="yajO"
                     /locus_tag="ACHKAU_000155"
     CDS             54112..55086
                     /gene="yajO"
                     /locus_tag="ACHKAU_000155"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO5529295.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(55140..55658)
                     /gene="pgpA"
                     /locus_tag="ACHKAU_000156"
     CDS             complement(55140..55658)
                     /gene="pgpA"
                     /locus_tag="ACHKAU_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO5529296.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(55636..56613)
                     /gene="thiL"
                     /locus_tag="ACHKAU_000157"
     CDS             complement(55636..56613)
                     /gene="thiL"
                     /locus_tag="ACHKAU_000157"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO5529297.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(56691..57110)
                     /gene="nusB"
                     /locus_tag="ACHKAU_000158"
     CDS             complement(56691..57110)
                     /gene="nusB"
                     /locus_tag="ACHKAU_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO5529298.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(57130..57600)
                     /gene="ribE"
                     /locus_tag="ACHKAU_000159"
     CDS             complement(57130..57600)
                     /gene="ribE"
                     /locus_tag="ACHKAU_000159"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO5529299.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(57689..58792)
                     /gene="ribD"
                     /locus_tag="ACHKAU_000160"
     CDS             complement(57689..58792)
                     /gene="ribD"
                     /locus_tag="ACHKAU_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO5529300.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(58796..59245)
                     /gene="nrdR"
                     /locus_tag="ACHKAU_000161"
     CDS             complement(58796..59245)
                     /gene="nrdR"
                     /locus_tag="ACHKAU_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO5529301.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            59396..59935
                     /gene="yajI"
                     /locus_tag="ACHKAU_000162"
     CDS             59396..59935
                     /gene="yajI"
                     /locus_tag="ACHKAU_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5529302.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            60234..61118
                     /gene="tsx"
                     /locus_tag="ACHKAU_000163"
     CDS             60234..61118
                     /gene="tsx"
                     /locus_tag="ACHKAU_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5529303.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(61295..61642)
                     /gene="yajD"
                     /locus_tag="ACHKAU_000164"
     CDS             complement(61295..61642)
                     /gene="yajD"
                     /locus_tag="ACHKAU_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO5529304.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(61771..62742)
                     /gene="secF"
                     /locus_tag="ACHKAU_000165"
     CDS             complement(61771..62742)
                     /gene="secF"
                     /locus_tag="ACHKAU_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO5529305.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(62753..64600)
                     /gene="secD"
                     /locus_tag="ACHKAU_000166"
     CDS             complement(62753..64600)
                     /gene="secD"
                     /locus_tag="ACHKAU_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO5529306.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(64628..64960)
                     /gene="yajC"
                     /locus_tag="ACHKAU_000167"
     CDS             complement(64628..64960)
                     /gene="yajC"
                     /locus_tag="ACHKAU_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO5529307.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(64983..66110)
                     /gene="tgt"
                     /locus_tag="ACHKAU_000168"
     CDS             complement(64983..66110)
                     /gene="tgt"
                     /locus_tag="ACHKAU_000168"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO5529308.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(66166..67236)
                     /gene="queA"
                     /locus_tag="ACHKAU_000169"
     CDS             complement(66166..67236)
                     /gene="queA"
                     /locus_tag="ACHKAU_000169"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO5529309.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            67329..67910
                     /gene="acpH"
                     /locus_tag="ACHKAU_000170"
     CDS             67329..67910
                     /gene="acpH"
                     /locus_tag="ACHKAU_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO5529310.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(67915..69732)
                     /gene="malZ"
                     /locus_tag="ACHKAU_000171"
     CDS             complement(67915..69732)
                     /gene="malZ"
                     /locus_tag="ACHKAU_000171"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO5529311.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(69888..71261)
                     /gene="proY"
                     /locus_tag="ACHKAU_000172"
     CDS             complement(69888..71261)
                     /gene="proY"
                     /locus_tag="ACHKAU_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO5529312.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(71337..72656)
                     /gene="brnQ"
                     /locus_tag="ACHKAU_000173"
     CDS             complement(71337..72656)
                     /gene="brnQ"
                     /locus_tag="ACHKAU_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO5529313.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(73063..74358)
                     /gene="phoR"
                     /locus_tag="ACHKAU_000174"
     CDS             complement(73063..74358)
                     /gene="phoR"
                     /locus_tag="ACHKAU_000174"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO5529314.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(74416..75105)
                     /gene="phoB"
                     /locus_tag="ACHKAU_000175"
     CDS             complement(74416..75105)
                     /gene="phoB"
                     /locus_tag="ACHKAU_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO5529315.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            75295..76497
                     /gene="sbcD"
                     /locus_tag="ACHKAU_000176"
     CDS             75295..76497
                     /gene="sbcD"
                     /locus_tag="ACHKAU_000176"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO5529316.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            76494..79637
                     /gene="sbcC"
                     /locus_tag="ACHKAU_000177"
     CDS             76494..79637
                     /gene="sbcC"
                     /locus_tag="ACHKAU_000177"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO5529317.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETLEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            79763..80947
                     /gene="araJ"
                     /locus_tag="ACHKAU_000178"
     CDS             79763..80947
                     /gene="araJ"
                     /locus_tag="ACHKAU_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO5529318.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(81090..81998)
                     /gene="mak"
                     /locus_tag="ACHKAU_000179"
     CDS             complement(81090..81998)
                     /gene="mak"
                     /locus_tag="ACHKAU_000179"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO5529319.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            82123..83034
                     /gene="rdgC"
                     /locus_tag="ACHKAU_000180"
     CDS             82123..83034
                     /gene="rdgC"
                     /locus_tag="ACHKAU_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO5529320.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(83112..83195)
                     /locus_tag="ACHKAU_000181"
     CDS             complement(83112..83195)
                     /locus_tag="ACHKAU_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529321.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(83192..83533)
                     /locus_tag="ACHKAU_000182"
                     /pseudo
     CDS             complement(83192..83533)
                     /locus_tag="ACHKAU_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(83681..83965)
                     /gene="ppnP"
                     /locus_tag="ACHKAU_000183"
     CDS             complement(83681..83965)
                     /gene="ppnP"
                     /locus_tag="ACHKAU_000183"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO5529322.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(84037..84714)
                     /gene="aroM"
                     /locus_tag="ACHKAU_000184"
     CDS             complement(84037..84714)
                     /gene="aroM"
                     /locus_tag="ACHKAU_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO5529323.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(84972..85163)
                     /gene="yaiA"
                     /locus_tag="ACHKAU_000185"
     CDS             complement(84972..85163)
                     /gene="yaiA"
                     /locus_tag="ACHKAU_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO5529324.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(85213..85737)
                     /gene="aroL"
                     /locus_tag="ACHKAU_000186"
     CDS             complement(85213..85737)
                     /gene="aroL"
                     /locus_tag="ACHKAU_000186"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO5529325.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(85920..86378)
                     /gene="yaiI"
                     /locus_tag="ACHKAU_000187"
     CDS             complement(85920..86378)
                     /gene="yaiI"
                     /locus_tag="ACHKAU_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO5529326.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            86498..87307
                     /gene="proC"
                     /locus_tag="ACHKAU_000188"
     CDS             86498..87307
                     /gene="proC"
                     /locus_tag="ACHKAU_000188"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO5529327.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(87324..88439)
                     /gene="adrA"
                     /locus_tag="ACHKAU_000189"
                     /gene_synonym="dgcC"
     CDS             complement(87324..88439)
                     /gene="adrA"
                     /locus_tag="ACHKAU_000189"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO5529328.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(88541..88861)
                     /gene="psiF"
                     /locus_tag="ACHKAU_000190"
     CDS             complement(88541..88861)
                     /gene="psiF"
                     /locus_tag="ACHKAU_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO5529329.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(88980..90395)
                     /gene="phoA"
                     /locus_tag="ACHKAU_000191"
     CDS             complement(88980..90395)
                     /gene="phoA"
                     /locus_tag="ACHKAU_000191"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO5529330.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(90496..90756)
                     /gene="iraP"
                     /locus_tag="ACHKAU_000192"
     CDS             complement(90496..90756)
                     /gene="iraP"
                     /locus_tag="ACHKAU_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO5529331.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(90938..91141)
                     /locus_tag="ACHKAU_000193"
     CDS             complement(90938..91141)
                     /locus_tag="ACHKAU_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529332.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            91219..92313
                     /gene="ddlA"
                     /locus_tag="ACHKAU_000194"
     CDS             91219..92313
                     /gene="ddlA"
                     /locus_tag="ACHKAU_000194"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5529333.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(92337..92549)
                     /gene="yaiZ"
                     /locus_tag="ACHKAU_000195"
     CDS             complement(92337..92549)
                     /gene="yaiZ"
                     /locus_tag="ACHKAU_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO5529334.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            92809..93117
                     /gene="yaiY"
                     /locus_tag="ACHKAU_000196"
     CDS             92809..93117
                     /gene="yaiY"
                     /locus_tag="ACHKAU_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO5529335.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(93176..94270)
                     /gene="yaiW"
                     /locus_tag="ACHKAU_000197"
     CDS             complement(93176..94270)
                     /gene="yaiW"
                     /locus_tag="ACHKAU_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO5529336.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(94283..95503)
                     /gene="sbmA"
                     /locus_tag="ACHKAU_000198"
     CDS             complement(94283..95503)
                     /gene="sbmA"
                     /locus_tag="ACHKAU_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO5529337.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            95855..97012
                     /gene="ampH"
                     /locus_tag="ACHKAU_000199"
     CDS             95855..97012
                     /gene="ampH"
                     /locus_tag="ACHKAU_000199"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO5529338.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(97013..97636)
                     /gene="iprA"
                     /locus_tag="ACHKAU_000200"
     CDS             complement(97013..97636)
                     /gene="iprA"
                     /locus_tag="ACHKAU_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO5529339.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(97724..100421)
                     /locus_tag="ACHKAU_000201"
                     /pseudo
     CDS             complement(97724..100421)
                     /locus_tag="ACHKAU_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(100451..>100657)
                     /locus_tag="ACHKAU_000202"
     CDS             complement(100451..>100657)
                     /locus_tag="ACHKAU_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5529340.1"
                     /translation="KGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECD
                     YNRWRRHSLCGGLSPEQFENKNLA"
BASE COUNT        24620 a        26553 c        24836 g        24648 t
ORIGIN      
        1 tcacgccgca tccggcatga acaacgcgca ctttgtcagt aatctcgggg caactaaatg
       61 tcgccccctt tggttattac cctaccgctg gcgtcgggtc atcgcctttg tagtcgcgtt
      121 tgacttccgt cacctcatcg cccttctcgt tgtgcagatg cacttcaagc tggttaaagg
      181 caatgttgat gtcgttttca cggcacagct gatcgatagt acggttcagc tcatcgacag
      241 tacgactacg gtcacgcagt tcacgcacat acagacgcag ctcatgatcc aacgtgctgg
      301 caccaaacgc cgtaaagaag acttccggca ttggttcgtg catcaccctt gggtgctcag
      361 tcgccgcctt cagtaacact ttacgcactt tttccagatc ggagccatag gccacgccga
      421 gacggatcac cagacgcgta gtagtgtcag tcaacgacca gttgatcaga cgctcggtaa
      481 caaacgcttt gttcgggatg atcacttctt tgcgatcgaa atcggtaatc gttgtcgcac
      541 gaatacggat cttacttacc gtccccgaga agctaccaat ggttaccgta tcgccaatac
      601 gcaccggacg ttcgaacaga atgatcaaac cggagacgaa gttaccgaaa atttcttgta
      661 aaccaaaacc aagacctacg gataatgctg cggccagcca ctggagttta tcccaagaga
      721 cgcccagcga tccgaacacc gtcatcgcac caacggcaat aatgatgtag ttaaggatgg
      781 tagtaatggc atacgacgcg ccctgacgca tattcagtcg cgagagcacc agcacttcca
      841 gtaaaccagg caggttgcga atcaacgccc aggccaccat tgaggcgata atcgcaaaca
      901 acagactgcc catggtgacg tttttcacca ccgcagcgcc agcttcagtg ccgttgtaat
      961 gccagagcgt gatgctgtcg agatagctga acacggtgat caaatcggac caaattgccc
     1021 agaacatgac accgaacagc gcaaacatca gcaacatggt aatacgcagc gtctgctggt
     1081 taacctgttc cagtgcaatg gtgggttctt ccggcggttc ggcaccttct gcgccctctt
     1141 tcaccagatt ctgccgacgc gccaacgcac gacgccaggc gatacgccgc gccgctacgc
     1201 ttaagccgcg caataccgtc tggtagagca agttccagat gatcaccaga taaacggttt
     1261 caatccaacg cccggagagg cgcagcgtgg tgtagaaata acccgtggca gtcagcacca
     1321 tcaatgcaat cgggataatc gacagcacgg taatggtgac cagtcgcatg gtgtgcgact
     1381 ctttatcacg ccagctttcg cggcacattg gccataccag gaaggcaatc agcagcaggt
     1441 tgaagaaaat catcgcctgc cccagcacat catccatcag atgcagcggg gaaagttctg
     1501 ccaccacaga ccagaaatgg ataggcagca acgcgagact gatgcggaca atttgccgac
     1561 gccagtggct ggtctgctgc tccggcatgc cgaagtgacg cacggcaacg ccatttttct
     1621 ccagtacctt ccagcacagg ccaaacacca gccagaaaat cgccagtttt ttactgaacg
     1681 accatagcag ttcgctgatg ttgagctgca tggtcaacag aatcaggcca accgcgagaa
     1741 taatcaggca caccggcagc gcgcggatca ggtcgataag gatcgctttt ggtgtgttga
     1801 gctggctatc gttacgcagg gaacccacag cggaagccag tttctgttga tacgctttca
     1861 gccagcccag acgccagtgg atcagcccgg caatcaacag cagcggcaaa ccggcgagga
     1921 aagcgataaa aacggcaggc caggcttttt cccagttcac cgtgattttc atcgacttaa
     1981 attcatcttt caggctttgc gggaacgctt tgatccagtc ccagtccatt ggacggttac
     2041 tgttcaccca aaagatttgc tgagtcagga tggatttcag gtttttcgat acgctcatta
     2101 actgctgctg gttgatttgc aggttaatgg ccatcatcag ctggttaccc aactgtttgt
     2161 tgagttgatc cagcaattcg cgacgcatat caaccacttg caataacgca tcgtgaactt
     2221 cgctgttgac ttcgttggtg tgaccttctt ccagtttgtt gacgaacgca tcgctctgga
     2281 agagtgcatc acgctgctgg ttaacttcaa actgttcgag acgcaaatcc gcgatgcggt
     2341 tggtcatgtt ttccagttca tccgccgagg gcagcgtttg ttgttgctgg taaaggatac
     2401 gagacaacag caggctgccc ttcaggacgg caatctgctc tttaatattg cgttccgatt
     2461 gcagcgcccg ctccagccag tttttgactt taatgttttg ctgcatcaac tgattaccgt
     2521 tttcagtcgc ggtaatcaga cgctgactta actgctggtt aatttccagt tcctgtttca
     2581 ccagcggatt agcctgaata cgcgcggctt catccgggga gacggcttcc tgcgccgttt
     2641 tttcagttaa agtcaggcgc ttgctgttta ccgcttcttg caacagctgc aactggtgct
     2701 ccagacgggc gctgttcgcc gtcacgtaat cacgttgctt ttgcaaggta tcctgcaaga
     2761 cggtgttccc ttccaggctt ttacgctgct ggtcaatctc cgcattcagc aacgcctgct
     2821 gagcctgcat taacactttc tggctgggac gtaaggcagt ctcgccgaca tcagtcccat
     2881 ccagacgact gcgaatttgt tgcagctgct gcgaagcgtt atacatcgca ttttgcacgc
     2941 gttcgggctg cgtctgtaac gaaaccagct ggctgttata agacgccaga tcgttttgtg
     3001 cgttttgcaa atcgtccagc gcctgggcaa cgcgagtttc cagctggcgc aacgaaagcg
     3061 tactcagaat tttgcgcgtt tcttcgtcgt tatcgacatc gctaagtgct gttaacgccg
     3121 cggtcgcctg gcgcattttt tccggcgctt cagcgacttt ttgtcgtagc tgaactgtct
     3181 cttcttttat gcgatcgatt ttatcgaggg tggctaatgt atctgtcaga tcctgctgca
     3241 ccagtttgtc ctgagcagaa agatcttttt gtttatttag tgagtcaagt tgcgcctgca
     3301 ggtccgcttt tgtcggcaga tcaccattcg atgacgcccg cgcaaacgcc gtgttctggc
     3361 ataacaagac aaaaacaaaa gcaataaatg ctgaaaaaac aaaatgccgt gatcgtttgt
     3421 aatactggaa catagtcatg atgaatgaag gtttctgaac ctgaagaacg acctgaaaaa
     3481 gtcaaaccgc aagaatatca cgacgcagtg aaccagaata gcaacgacga aaatgtccag
     3541 gaaaaatcct ggagtcagat tcagggttat tcgttagtgg caggattacg aagcgtgggg
     3601 cacaggagat acatctccag taagatggca acgtaatcgc gggcttcttt tttaagatca
     3661 aaagattgcg gggcaaagag ccagttttcc atcaggccgg aaatatagcc gcgcataata
     3721 attgctgcgc gacgcgtcat taaatccgca ggcaacattt tcgcttcaat acaatgtttt
     3781 aacgtttgtt ctatacggtc ataactttcc agacagagat tacgttgtgc ctgttgcaca
     3841 acagccattt ctccgacaaa ttcgcatttg tggaatataa tctccatcaa taatcgacgc
     3901 cgttcttctg tcaccgtgga ttcaagaaca tgaattaata tctctcttaa tactgagagt
     3961 ggatcgccag ggaattttgc ctgatactca agctctagtt caccaatatt ggattctgac
     4021 agttcccaga tctcactgaa caaatccgac ttgtctttaa aatgccagta gattgcaccg
     4081 cgcgtaacgc cagctgcttt tgcaatctca cccagcgagg tggatgatac cccctgctgt
     4141 gagaaaagac gtagagccac atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg
     4201 gtttttcgtg ccatatgttc gtgaatttac aggcgttaga tttacataca tttgtgaatg
     4261 tatgtaccat agcacgacga taatataaac gcagcaatgg gtttattaac ttttgaccat
     4321 tgaccaattt gaaatcggac actcgaggtt tacatatgaa caaaaacaga gggtttacgc
     4381 ctctggcggt cgttctgatg ctctcaggca gcttagccct aacaggatgt gacgacaaac
     4441 aggcccaaca aggtggccag cagatgcccg ccgttggcgt agtaacagtc aaaactgaac
     4501 ctctgcagat cacaaccgag cttccgggtc gcaccagtgc ctaccggatc gcagaagttc
     4561 gtcctcaagt tagcgggatt atcctgaagc gtaatttcaa agaaggtagc gacatcgaag
     4621 ctggtgtctc tctctatcag attgatcctg cgacctatca ggcgacatac gacagtgcga
     4681 aaggtgatct ggcgaaagcc caggctgcag ccaatatcgc gcaattgacg gtgaatcgtt
     4741 atcagaaact gctcggtact cagtacatca gtaagcaaga gtacgatcag gctctggctg
     4801 atgcgcaaca ggcgaatgct gcggtaactg cggcgaaagc tgccgttgaa actgcgcgga
     4861 tcaatctggc ttacaccaaa gtcacctctc cgattagcgg tcgcattggt aagtcgaacg
     4921 tgacggaagg cgcattggta cagaacggtc aggcgactgc gctggcaacc gtgcagcaac
     4981 ttgatccgat ctacgttgat gtgacccagt ccagcaacga cttcctgcgc ctgaaacagg
     5041 aactggcgaa tggcacgctg aaacaagaga acggcaaagc caaagtgtcg ctgatcacca
     5101 gtgacggcat taagttcccg caggacggta cgctggaatt ctctgacgtt accgttgatc
     5161 agaccactgg gtctatcacc ctacgcgcta tcttcccgaa cccggatcac actctgctgc
     5221 cgggtatgtt cgtgcgcgca cgtctggaag aagggcttaa tccaaacgct attttagtcc
     5281 cgcaacaggg cgtaacccgt acgccgcgtg gtgatgccac cgtactggta gttggcgcgg
     5341 atgacaaagt ggaaacccgt ccgatcgttg caagccaggc tattggcgat aagtggctgg
     5401 tgacagaagg tctgaaagca ggcgatcgcg tagtaataag tgggctgcag aaagtgcgtc
     5461 ctggtgtcca ggtaaaagca caagaagtta ccgctgataa taaccagcaa gccgcaagcg
     5521 gtgctcagcc tgaacagtcc aagtcttaac ttaaacagga gccgttaaga catgcctaat
     5581 ttctttatcg atcgcccgat ttttgcgtgg gtgatcgcca ttatcatcat gttggcaggg
     5641 gggctggcga tcctcaaact gccggtggcg caatatccta cgattgcacc gccggcagta
     5701 acgatctccg cctcctaccc cggcgctgat gcgaaaacag tgcaggacac ggtgacacag
     5761 gttatcgaac agaatatgaa cggtatcgat aacctgatgt acatgtcctc taacagtgac
     5821 tccacgggta ccgtgcagat caccctgacc tttgagtctg gtactgatgc ggatatcgcg
     5881 caggttcagg tgcagaacaa actgcagctg gcgatgccgt tgctgccgca agaagttcag
     5941 cagcaagggg tgagcgttga gaaatcatcc agcagcttcc tgatggttgt cggcgttatc
     6001 aacaccgatg gcaccatgac gcaggaggat atctctgact acgtagcggc gaatatgaaa
     6061 gatgccatca gccgtacgtc gggcgtgggt gatgttcagt tgttcggttc acagtacgcg
     6121 atgcgtatct ggatgaaccc gaatgagctg aacaaattcc agctaacgcc ggttgatgtc
     6181 attaccgcca tcaaagcgca gaacgcccag gttgcggcgg gtcagctcgg tggtacgccg
     6241 ccggtgaaag gccaacagct taacgcctct attattgctc agacgcgtct gacctctact
     6301 gaagagttcg gcaaaatcct gctgaaagtg aatcaggatg gttcccgcgt actgctgcgt
     6361 gatgtggcga agattgagct gggtggtgag aactacgaca tcatcgcaga gtttaacggc
     6421 caaccggctt ccggtctggg gatcaagctg gcgaccggtg caaacgcgct ggataccgct
     6481 gcggcaatcc gtgctgaact ggcgaagatg gaaccgttct tcccgtcggg tctgaaaatt
     6541 gtttacccgt acgacaccac gccgttcgtg aaaatctcta ttcacgaagt ggttaaaacg
     6601 ctggtcgaag cgatcatcct cgtgttcctg gttatgtatc tgttcctgca gaacttccgc
     6661 gcgacgttga ttccgaccat tgccgtaccg gtggtattgc tcgggacctt tgccgtcctt
     6721 gccgcctttg gcttctcgat aaacacgcta acaatgttcg ggatggtgct cgccatcggc
     6781 ttgttggtgg atgacgctat cgttgtggta gaaaacgtgg agcgtgttat ggcggaagaa
     6841 ggtttgccgc caaaagaagc tacccgtaag tcgatggggc agattcaggg cgctctggtc
     6901 ggtatcgcga tggtactgtc ggcggtattc gtaccgatgg ccttctttgg cggttctact
     6961 ggtgctatct atcgtcagtt ctctattacc attgtttcag caatggcgct gtcggtactg
     7021 gtagcgttga tcctgactcc ggctctttgt gccaccatgc tgaaaccgat tgccaaaggc
     7081 gatcacgggg aaggtaaaaa aggcttcttc ggctggttta accgcatgtt cgagaagagc
     7141 acgcaccact acaccgacag cgtaggcggt attctgcgca gtacggggcg ttacctggtg
     7201 ctgtatctga tcatcgtggt cggcatggcc tatctgttcg tgcgtctgcc aagctccttc
     7261 ttgccagatg aggaccaggg cgtatttatg accatggttc agctgccagc aggtgcaacg
     7321 caggaacgta cgcagaaagt gctcaatgag gtaacgcatt actatctgac caaagaaaag
     7381 aacaacgttg agtcggtgtt cgccgttaac ggcttcggct ttgcgggacg tggtcagaat
     7441 accggtattg cgttcgtttc cttgaaggac tgggccgatc gtccgggcga agaaaacaaa
     7501 gttgaagcga ttaccatgcg tgcaacacgc gctttctcgc aaatcaaaga tgcgatggtt
     7561 ttcgccttta acctgcccgc aatcgtggaa ctgggtactg caaccggctt tgactttgag
     7621 ctgattgacc aggctggcct tggtcacgaa aaactgactc aggcgcgtaa ccagttgctt
     7681 gcagaagcag cgaagcaccc tgatatgttg accagcgtac gtccaaacgg tctggaagat
     7741 accccgcagt ttaagattga tatcgaccgg gaaaaagcgc aggcgctggg tgtttctatc
     7801 aatgacatta acaccactct gggcgctgca tggggcggta gctatgtgaa cgactttatc
     7861 gaccgcggtc gtgtgaagaa agtttacgtc atgtcagaag cgaaataccg tatgctgccg
     7921 gatgatatcg gcgactggta tgttcgtgct gctgatggtc agatggtgcc gttctcggca
     7981 ttctcctctt ctcgttggga gtacggttcg ccgcgtctgg aacgttacaa tggcctgcca
     8041 tccatggaaa tcttaggcca ggcggcaccg ggtaaaagta ccggtgaagc aatggagctg
     8101 atggaacaac tggcgagcaa actgcctacc ggtgttggct atgactggac ggggatgtcc
     8161 tatcaggaac gcctctccgg caaccaggca ccttcactgt acgcgatttc gttgattgtc
     8221 gtattcctgt gtctggcggc gctgtacgag agctggtcga ttccgttctc cgttatgctg
     8281 gtcgttccgc tgggggttat cggtgcgttg ctggctgcca ccttccgtgg cctgaccaat
     8341 gacgtttact tccaggtagg cctgctcaca accattgggt tgtcggcgaa gaacgcgata
     8401 cttatcgtcg aattcgccaa agacttgatg gataaagaag gtaaaggtct gattgaagcg
     8461 acgcttgatg cagtgcggat gcgtttacgt ccgatcctga tgacctcgct ggcgtttatc
     8521 ctcggcgtta tgccgctggt tatcagtact ggtgctggtt ccggcgcgca gaacgcagta
     8581 ggtaccggtg taatgggcgg gatggtgacc gcaacggtac tggcaatctt cttcgttccg
     8641 gtattctttg tggtggttcg ccgccgcttt agccgcaaga atgaagatat cgagcacagc
     8701 catactgtcg atcatcattg atacaacgtg taatcactaa ggccgcgcaa gcggcctttt
     8761 ttatgcataa cctacgaaca ttaaggagta attgaaccac caactcagga tctcatacga
     8821 aaaccagtat taaccacgga taaaattcat aaaaaatact gattgttagt taatttatat
     8881 taagtagcgc taatagattt aataatccat aatcatttag aggctattct taattatttg
     8941 cggtaattct ttattcattc ctcggttatt acgtcatatt cagagcaatc ctggtattag
     9001 tgtcaccaat ttcatctggc gataatcctg aaatgttatg aacagttcga gcaaactgct
     9061 tttacctgct gcgggttagt gctaatatga aaaagtgagt cctgtcccga ttccttccta
     9121 attgtaattt ttcgtaataa tgcgatgaaa acctgcaaag agtggcttat agttaagcta
     9181 acaaacgaga gggcaagtcc aggtcagtaa gttttttcca tcccgaaagg tgtccgttag
     9241 ttcaaccact aagaagggga cgcgttatgg atgaatactc acccaaaaga catgatatcg
     9301 cacagcttaa gtttctctgt gaaaccctgt atcatgactg tcttgcaaac cttgaagaaa
     9361 gcaatcatgg ctgggttaac gacccaacct cggcgatcaa cctccagttg aatgaactga
     9421 ttgagcatat tgcgaccttc gcacttaatt acaaaattaa gtataatgaa gacaataagc
     9481 tcattgagca gatcgacgaa tatctggatg acacctttat gttgttcagc agttatggta
     9541 ttaatatgca ggatcttcag aaatggcgga agtcaggtaa tcgactattc cgttgttttg
     9601 tcaatgcgac gaaagagaat cctgcgagtt tatcttgtta gaattattac aaccatgggt
     9661 agaagtatgt ccgaaaaacc tttaacgaaa accgattatt taatgcgttt acgtcgttgc
     9721 cagacaattg acacgctgga gcgtgttatc gagaaaaata aatacgaatt atcagataat
     9781 gaactggcgg tattttactc agccgcagat caccgcctcg ccgaattgac catgaataaa
     9841 ctatacgaca agatcccttc ctcagtatgg aaatttattc gctaataaat aattcgcttt
     9901 cggagctata accggctgtt tattaagaat tttatacttt ttcaccatga agacataccc
     9961 tatgtgatct ttatcacaca gatgtaatag gaacgttctc ttcactgact tttcgtctta
    10021 ctgtgttgcc gcattttcag caaccggagg tcagtaatga gcacagaaaa agaaaagatg
    10081 attgctggtg agttgtatcg ctcggcagat gagacgttat ctcgcgatcg cctgcgcgct
    10141 cgtcagctta ttcaccgata caatcattcc ctggcggaag agcacacatt acgccagcaa
    10201 attctcgctg atctattcgg tcaggtgaca gaggcttata ttgagccaac gtttcgctgt
    10261 gactatggct ataacatttt tctcggtaat aattttttcg ccaacttcga ttgcgtgatg
    10321 cttgatgtct gccctattcg catcggtgat aactgtatgt tggcaccagg cgttcatatc
    10381 tacacggcaa cacatcccat cgaccctgta gcacgtaata gcggtgctga actggggaaa
    10441 cccgtcacca tcggtaataa cgtctggatt ggcggacgcg cggtcattaa ccctggtgtg
    10501 accattggtg ataacgtcgt ggtagcctca ggtgcagttg tcacaaaaga tgtcccggac
    10561 aacgttgtcg tgggcggtaa tccagccaga ataattaaaa aattgtaatc ggtttttcgc
    10621 aactgttatg caaaattgtg gtagatctgt tacttcccct ctactattcc cacgttaaaa
    10681 tagggtgttc cctggaaagt tgcagatacc acgaaggcaa acgatgaccg aaatacaacg
    10741 cctgctgacc gaaacgattg agtctctgaa tacccgcgaa aaacgcgaca acaaaccccg
    10801 ctttagtatc agttttatcc gtaaacatcc ggggctgttt atcggtatgt acgttgcttt
    10861 ttttgccacc ctggcggtga tgttgcagtc cgaaacgctg tcaggctctg tctggctact
    10921 ggttgtatta tttatcctgc ttaatggttt cttctttttc gatgtctacc cacgctaccg
    10981 ctatgaagat atcgacgtgc tggatttccg cgtttgctat aacggcgaat ggtacaacac
    11041 gcgctttgta cctgccgcgc tggttgaagc catcttgaac tctccgcgtg tcgcggatgt
    11101 tcataaggaa caactgcaaa aaatgatcgt ccgtaaaggt gaactgtctt tttacgatat
    11161 ttttaccctc gctcgcgccg aatcaacatc ttaagttagg gttacatacc aggcgtaaag
    11221 ctctgcgcct ggtcaaatga caatgatcgt ttccacccat cacttcatga aataccagct
    11281 ctacctcctt atctcctgcc agcctttttc cacaatcaga tatactttcc ctacactgtg
    11341 ttaataagga tatgctggtg agaacacgac atctggtcgg ccttatttcg ggagtactga
    11401 ttctttcagt attgctgcct gtcggcttaa gcatctggct ggcccatcag caggtagaaa
    11461 catcgtttat tgaagagctg gatacctatt cctcccgcgt cgctattcgg gccaataagg
    11521 tggcgacaca agggaaagat gcgctgcaag agctggaaag atggcaaggc gctgcctgta
    11581 gcgaagccca tctcatggaa atgcgtcggg tatcttacag ttatcgctat attcaggaag
    11641 tggtttatat cgataacaac gttccccagt gttcgtctct ggagcatgaa agtccgcccg
    11701 ataccttccc cgagccaggt aaaatttcga aagatggtta tcgtgtctgg ttaacatcgc
    11761 ataacgattt aggcattatc cgttacatgg tcgccatggg aacggcacat tatgtcgtca
    11821 tgatcgaccc cgcttccttt attgatgtca ttccctatag ctcatggcaa attgatgccg
    11881 ccattattgg caatgcccat aacgttgtca taaccagcag cgatgaaatt gctcagggaa
    11941 ttattaccag gctacaaaaa acacccggtg agcatatcga aaataatgga atcatttacg
    12001 atatcctgcc cttcccggag atgaatattt cgatcatcac ctgggcttca acgaaaatgt
    12061 tgcagaaagg ctggcatcgg caagtcttta tttggttacc gctcgggttg gtgattggcc
    12121 agctggcagc gatgtttgtg ctgcgtattt tgcgccgtat ccagtcaccg catcatcggc
    12181 tgcaggatgc tatcgaaaat cgtgatattt gcgtgcacta tcagccgatt gtctccttag
    12241 ccaatggcaa aattgtcggt gctgaggcac tggcgcgctg gccgcagaca gacggtagtt
    12301 ggttgtcacc agatagtttt attccgctgg cacagcaaac gggcctttct gagccattga
    12361 cgctactgat tataagaagc gtctttgaag atatgggcga ctggctgcgt cagcatccac
    12421 agcagcatat ttcgatcaat cttgaatcca ccgtgctcac ctcggaaaaa atcccgcaat
    12481 tgctgcgtga aatgatcaat cactatcagg ttaatcccag acagatcgcg cttgaactca
    12541 ctgaacgcga gtttgccgat ccgaaaacca gcgccccgat aatttctcgc taccgggagg
    12601 cgggccatga aatttatctt gatgattttg gtacgggata ttcaagttta agttatttac
    12661 aggatctgga tgtcgacatt ctgaagatcg ataaatcttt cgttgatgcg ctggaatata
    12721 aaaatgtcac gccgcatatc atcgaaatgg caaaaacact gaaactgaaa atggtagcgg
    12781 agggaatcga aaccagtaaa caagaagagt ggctacgcca gcatggcgtg cactacggtc
    12841 agggctggct ctacagcaag gcattaccga aagaagattt cttacgctgg gccgagcaac
    12901 atttgtgaat ttttgggctg acggcacgcc gcacagcccg tcacgctacc tattcgtcga
    12961 tgattgactc gaatccgcca tagatcattc gcttgccgtc aaacggcatg gactcgccga
    13021 actctttcat ccgtgggtcc gacatcatct tttgattagc agcgtcgcgg acctcttttg
    13081 aagggtattc aatccagcta aagacaacct cttcattctc ttccgctttc accgccatac
    13141 gaaaatcggt cactttgcca tccggtacat cgctggccca gcattcgaca atacgaagcg
    13201 cgccaaactc tttaaacaat ggcgcagcct tagcggccat ttcccgatag gcatcctttt
    13261 tatcggcagg aacggcaacc acaaaaccat caacatactt catcagacat acctccgaag
    13321 cgtactgcgc agccagacaa ttcttgcgac gtcagttgtg gttaaagttt agccatgttg
    13381 gttttttttg acgaaacggt gggaggcaga aatgggtggg ggccctgcca gctacatccc
    13441 ggcacacgcg tcatctgcct tggctgcttc cttccggacc tgacctggta aacagagtag
    13501 cgttgcggga gaaccaacag gcccccattg agagcgttga gaaccaacgc gcaggcgcat
    13561 tatcactgct gccccggcta attgcaaccc atcgcgtgcc agatgctggt ttcttgcgca
    13621 atgcgacttc actcgggcgt ttttccagat tatgctgaaa aattacctca ggaagaaaag
    13681 atggaaaaag aagattcatt tccccaacgc gtctggcaaa tcgtcgccgc tattcccgaa
    13741 ggctatgtca ccacttacgg tgatgtggcg aaactggcgg gatcgccccg cgccgcgcgc
    13801 caggtgggcg gtgtgttaaa gcgtctccct gaaggcagca ccttaccctg gcaccgggtg
    13861 gttaatcgcc acggcacaat ttcgctaacc ggaccggatt tacagcgtca gcgacaggca
    13921 ttactggcag aaggtgtgat ggtatcggga agcgggcaaa tcgacttgca gcgttatcgc
    13981 tggaactact aaccctctcc gggcggagag ggttaacgtc gcttaatatt gcgtaggagc
    14041 tggaactgcc gaagacggat tcacctgagt tggtgaagtc gaaggtacgg tagtcgttgc
    14101 accaccgctg gcctgaaccg gcacggcggt ttgctgcacc ggcaccaatg tcaggtcggc
    14161 tttagttccg cccttgttga tcaccggctg aacggtatcg gtaataaata ccagtttgtc
    14221 attcacggta atcgccgcac tcaacagaat acgcgcgttc ggctgaacat ctgccggatt
    14281 aaatggcaga acaaagctga atggtgactg tttaccttcg gtacgcaccg ctttctgcgc
    14341 cagcactttt gacggtgcat cggctaacga cgcgtcagaa agtgtcacgg tcagcacagc
    14401 atcaggcggc agtgcgactt tctgccggat ccagacggta ccggagacat tcggttgctg
    14461 gatagcaggt tgttgtgttg ctgaaataga tgtatttgca gccggggctg gcgtctgaat
    14521 atccgcgctt ttatctgcgc aagccgccaa cgcaatcgca accgctaaac cactggccat
    14581 gtgcacgagt ttcattcatt tctccttatt atcaatgcac cagcgggcta actttcctcg
    14641 ccggaagagt ggttaacaaa atagtaacgt caacaagtgt ggcacacatc acgcatttct
    14701 gcctgtaatt agcccgtaat tcagaccatt gcacccatcg gaccagtagc aaatttgcgt
    14761 tatactcaac tcactttggc ttgctgcggc agctttgtta ctggagagtt atatgagtca
    14821 ggcgctaaaa aatttactga cattgttaaa tctggaaaaa attgaggaag gactctttcg
    14881 cggccagagt gaagatttag gtttacgcca ggtgtttggc ggccaggtcg tgggtcaggc
    14941 cttgtatgct gcaaaagaga ccgtccctga agagcggctg gtacattcgt ttcacagcta
    15001 ctttcttcgc cctggcgata gtaagaagcc gattatttat gatgtcgaaa cgctgcgtga
    15061 cggtaacagc ttcagcgccc gccgggttgc tgctattcaa aacggcaaac cgatttttta
    15121 tatgacggcc tctttccagg caccagaagc gggtttcgaa catcaaaaaa caatgccgtc
    15181 cgcgccagcg cctgatggcc tgccttcgga aacgcaaatc gcccaatcgc tggcgcacct
    15241 gctgccgcca gtgcttaaag ataaattcat ctgcgatcgt ccgctggaag tccgtccggt
    15301 ggagtttcat aacccactga aaggtcacgt cgcagaacca catcgtcagg tgtggatccg
    15361 cgcaaatggt agcgtgccgg atgacctgcg cgttcatcag tatctgctcg gttacgcttc
    15421 tgatcttaac ttcctgccgg tagctctaca gccgcacggc atcggttttc tcgaaccggg
    15481 gattcagatt gccaccattg accattccat gtggttccat cgcccgttta atttgaatga
    15541 atggctgctg tatagcgtgg agagcacctc ggcgtccagc gcacgcggct ttgtgcgcgg
    15601 tgagttttat acccaggacg gcgtgctggt tgcctcgacc gttcaggaag gggtgatgcg
    15661 taatcacaat taaaaaaaat agccggaggt aaaaccgtcc ggctattttt tgcagtgctt
    15721 gttacgcgtt ataggcattc tcgccgtgac tgttgacatc cagcccttct cgctcctgct
    15781 cttccggtac acgcagacca accgtcagat ccgccagttt gtagccaata aatgccacca
    15841 caccggacca gacgatcgta atggcgatgc tttccagctg taccagcaac tgatggccca
    15901 tcgtcacacc ttcagcgaag cccacgccac ccagcgaact ggcggcaaaa atcccagtca
    15961 tgatacagcc gacaatgcca caaacgccgt gcacaccgaa gacatcgcag ggatcatcca
    16021 cccgcagcaa gcgtttgagc atagtaacgc cccacaagcc cgccagacca gctaccacgc
    16081 cgataatcaa cgcgccgcca accccaatgt agccacacgc tggcgtcacg ccgaccagac
    16141 cggcaatcgc gccagaacac gctcccagca gagaaggctt accacgcagc gcccattcac
    16201 cgaagatcca gccaagaatt gccgccgccg ttgcgaccac agtattcaca aatgccagtg
    16261 ccgcgatttc attcgccgtg cccgctgacc cggcgttaaa gccaaaccaa ccaatataga
    16321 gaatggcagt accggtgaag accattggca ggttgtgcgg tttaaacgcc tctttgccga
    16381 agcccacgcg tttgcctatc agatacgcgc ccaccagacc ggcgattgcg gcgttaatat
    16441 gcaccaccgt gccacccgcg aaatccagcg caccgtgaga agccagcaaa ccaccgcccc
    16501 acaccatatg cgcaatcgga atgtaagaga gcgtcagcca taccaccacg aaaatcaaca
    16561 cagctgagaa gcggattcgt tccgccagcg ccccaacgat caagccgacg gtaatgcagg
    16621 caaacgagcc ctgaaacgcc acgtggatat actgataaat gctgcccatc accgccgtca
    16681 gttcgatgtt ttttagcatc aaccagttaa tgttgccgaa gaagttgttg ccctcaccaa
    16741 acgccagcga gtaaccgtaa accacccaga gaatacagac cagtgcaaac gtcaccgtca
    16801 cctgcgtcag catcgacagc acgtttttgc cgcgaatcaa cccaccgtaa aacagggcaa
    16861 tccccggaat agtcataaac agcaccagcg cagtacaaat catcataaac gcattgtcgg
    16921 ctttatcggc caccgcaggt gcagccatta ccagtcccgg cagcatcgcc agtgaagcaa
    16981 gcccagtttt tatcgtcgct atcttcattt ttcgttcctg ttgctgtgtg ccagagatta
    17041 cagcgccgct tcgtcggctt cgccggtacg aatacgaatg acgcgttgca attcagcgac
    17101 gaagattttg ccgtcgccaa tttttccggt gtaagccgcc ttactgacga tatcgatcac
    17161 ttcatcgagt tggtcatcag caatcgccac atcaattttt acttttggca ggaagttgac
    17221 gctgtattcc gccccccggt acagctcggc atgccctttc tgacgcccga aacctttcac
    17281 ttcggtgacg gtcaggccct gaataccaat ggaagataac gcttcacgaa cgtcttccag
    17341 cttgaatggt ttgattatca cggtcaccag cttcatagat cccctccggt cagaattcgg
    17401 taatggttct gctacacgaa gaaggtattg caagcggtgt gccagaaatg aaaaacgaga
    17461 aagagagcag gaagttaaga agattgaaaa tgaaaacgca ctatgacagt gcacagtgcg
    17521 ttacattttt gcaccaacga tgaaatattg cgctattcag gcgctcaatg attcttcttc
    17581 acgcacgctg gctgccagct cttcgcccgc aagttgcagt tgatacatct gccagtagcg
    17641 tccctgggcc gccagcagtt gctggtgagt gccctgctcc acggcttgcc cacgatgaag
    17701 caccagaatg gtgtcggtat caacaatggt cgataagcgg tgagcaatca ccaccagcgt
    17761 ggtatgttca cgcacagccg ccagagcatg ttgaatcgcc tgttcagtac cggagtcaat
    17821 gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc tccaccaaca cgcgtgccag
    17881 tgccagcagt tgcttttgcc cgacggagag attattcccc tgctcgccca gcggcgtata
    17941 aataccatca ctcatgctgc gcgccagctc cgccagttgc acggtttcca gcgcctgcca
    18001 gacgcgttct tcggagatat cacgccccag cgtcacgttg gcgaggaagg tatccgccag
    18061 caccaccgga tcttgctgca ccattgccac gccctggcgc agcgcgctgt gacttagcga
    18121 acttaatgga cgaccatcaa ggcgaatctc accttccgtt agcgggtaat agcccatcaa
    18181 taaactggcg agggtgcttt tgccactgcc ggtatgcccg accagtgcca caaagttacg
    18241 cgaaggcaca gagagattaa tgttctttag caccagattg tcatcgcgat aagcaaatga
    18301 cacgttatcg acttcgatgg tgccactctg taacgggcga tcatcattgc catattgctg
    18361 gcgcggtccg tccatcagtt caaacacgcg ctcaccagca acaacagcct gttgcagcat
    18421 cgcctgttgc gtggtcagtt cgattaatgg ttcgttaagt cgcccaagat agctgataaa
    18481 cgcatacagc acgcccactt caatggtgcc gctagcggag aagccaaaca gcatcaacaa
    18541 gccacaaaga atgagcgatg aaaacagact cagcagcgga cgcagcagaa aaccgtcgag
    18601 gcgcagggtt tgcatcctgg ccatatagtg tgaacgactg gcctccccca tacgttcgcc
    18661 aaatcgcgcc tgctgacgga actgctggat aacgctcatg ccattgatga tttcgttaaa
    18721 gccgtcgttg atatccgcta gataggcgcg cacacgacgg acaatcggcg tgctgtaacg
    18781 ctggtatatc accattacca ccagcaccac cgggaaaatc attatcgcca ccagcgccat
    18841 tcgccagtcg aggctgaaca tcgccaccag catcgcgccc accagtgcgg cgctacgcag
    18901 gacagttgcc actacggtaa cgtagagatc gcggatcact tcagtgtcat tagtgacgcg
    18961 ggaaatcacc tgcccgacgg gttgggtatc aaactcgctt aatggctggc gcaatgcggc
    19021 atccatcacg tcggtacgca actgttgcac tacgcctact gccgcccgat taaacagcag
    19081 cgactgcgcg taatgtagcc cggcggcaaa cagttgtagc ccaacatacg ccgcagccag
    19141 ccctgcaacc attttcaacg gcaggttatt tttcgctacc atattgtcga taaaatagct
    19201 gataagcagc ggcccactga cttctgccgc tgccgcaacc cacatcatca ggaccgcaat
    19261 ccccagcggt ttacgccacg gcgaaccgta cgctaacaga cgcttgagag tcggccacag
    19321 ttggctaaaa ctacgcatcg acggcctcct cgcgaatttc cggagcgtcg tcgagcgccg
    19381 cctccagttg ttgatagcga tacatatcgc gataccagcc gctttgttgc gccagctcat
    19441 catgattgcc acgctgggcg atatgtccgt gctgcatcac aataatttca ctggcttccg
    19501 tcagtgccga aaggcgatgg gcactgatga ttaccgttct accctgtccc cactgacgca
    19561 ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc cgaaagcgca tcatcaagga
    19621 tgaggatttc cgcgttgact aataacgcac gagcaataga gatacgctgt ttttgcccgc
    19681 cggaaagcat cacgccgcgc tcgcccacct ctgtatcgta accttgcggt aaacgcaaaa
    19741 tatcgtcatg tacgctggct aaccgcgcga catgctcaat ctcttgctgg gtggcattcg
    19801 ggcaacccaa cgcgatgttg ttcgctacgg tgtcggaaaa aaggaacggt gtctggctaa
    19861 ctaccgccag gcggctacgc cagctatcga gttgtaactt cgtcagagga atatcatgaa
    19921 agcgaatatc cccctcgctg acgtcgaaat gacgctgaat gagcgacaac agggtacttt
    19981 tgccggaacc agtcggcccg cagataccca gcatctgacc gggtttcagg gcgaaattga
    20041 cgttttccag cgcacgatgg tcagtctgcg gatacgtgaa ctggtgaata tttacatcaa
    20101 gttcgccacg cccttccggc acgggttcgc taccatcgtt caccaccggc gcttccgcca
    20161 gcatcgcacg aatacggctg tacgcagcac taccacgttc cacaatgtta aacatccatg
    20221 ccagcgccag cattggccaa atcatcagac ctaaatacat cataaaactg gtgagctggc
    20281 ccagcgttaa actgccctgc accaccatcc aactaccgcc gccaatcgcc agcaagttcg
    20341 ccataccaat cgcgatatag attgtcgggt cgaaacgagc atcaatacgc gccacccgca
    20401 tgtttttttt gccggtatct tcggcatccg cggcaaataa cgccgactgg cgatcttcca
    20461 gaccaaaggc tttgatcatg cggatactgg tgaggctttc ctgggtgcgg tcattaagac
    20521 tggaaaacgc cgcctgtgcc agcttaaagc gttcatgcaa ggcatcgccg ttgcgcttaa
    20581 tcatgatcgc catcactggc atcggtaaca gggcaaataa ggtcaactgc cagctaattt
    20641 gcgtagacat cataatcaac acagcgcagc ccattaccag tgaatccacc agcgtcagca
    20701 ccccttctcc ggcggcaaac acgacgcgat cgacgtcatt ggtcgcacga gccatgagat
    20761 cacccgtgcg atgacgcagg taaaactccg ggtgctgccg gcttagctga cggtaataat
    20821 cttcacgcag ttcaacagcc agttggtaag acgcgccaaa cagcaatacg cgccagacat
    20881 aacgcaggag ataaaccaca acggcaatca gcaccatggt ggcgatccac atcaggatca
    20941 gcccggtagt aaagtgttgt tctgtcacgc catcgacaac aataccaacc acttttggcg
    21001 gaaccagttg cagcatcgcg ataatgacaa gcaaggcgac agccccgaga tagcgacgcc
    21061 attcccgacg gaaataccag cttaattgag caaataatcg cacgcgttgt gtcctgacct
    21121 gattctggat atttattcga tgggtaaaga agtggtgtat ttaatctgtt ccatcgcgaa
    21181 gctggaagtg acgtccgaca gccccggcac gctgtttacc agacgcttat aaaactcgtc
    21241 gtagcgtttc atgtcggcaa cctggacgcg catcagataa tcgtattcac cagccatgcg
    21301 ccagaacccc agcacttctg gcatttcggt aaccaccgtg acaaagcggc aataccattc
    21361 gctgctgtga tgttgcgttt ttatcagcac aaaagcggtc aggccgaggc ctattttttc
    21421 cggatccagc agggcgactt tgccgataag gataccgtcg tcctccagcc gtttcagacg
    21481 cttccagcaa ggggtggttg tcagattaac ggcttcagcc agtgcctgca aagagagggt
    21541 gcaatcctgc tgcagtaagg ccagcagctt atggtcaatt ttatctaaca tagcccttcc
    21601 acagagaatt tttttctctt ttaattctat tttaaaggtg aaatcacgac aatttattct
    21661 gtgcggttta aacaccacgg cacaaaatga caaattcaac gcaaaaaaaa ccgggcaatt
    21721 gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
    21781 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
    21841 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaacccgct accgacgctg
    21901 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
    21961 cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac cggcagcact
    22021 tcgaggcaat ccgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
    22081 tggatctgca agcgtgtaag atcgtcgtga ccgccacaga agcagatctt ggtgactctg
    22141 ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata gacaaatgcc
    22201 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg
    22261 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
    22321 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
    22381 tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
    22441 gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca
    22501 cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
    22561 atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg
    22621 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgattg ctcaaccgtc
    22681 ttaaccagta tcaacgtctg tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca
    22741 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
    22801 cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag cgcggacaat
    22861 tacgctttct ggtcacgccg gaatcgctac gcaatgcgat gatggaacag gcactggaaa
    22921 ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaggtgag ctgcgcactc
    22981 tgttacagcc gtttatgggc ggacaatggc aaaacgatac acccacgttg cgtattccct
    23041 actatcgccc gctcgaaccg ctacaaccag gctttttgcc cggccgtgcc gagcagcatc
    23101 tcgccgggca gatattttcc ggcctgaccc gcttcgataa taatactcag cgcccgattg
    23161 gcgatttagc gcatcactgg gaaacctcta ctgacgggtt acgctgggac ttttatcttc
    23221 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
    23281 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcatattg
    23341 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac tggcttgcgc
    23401 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcactg
    23461 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggagagc catgattatt
    23521 accatttgcg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
    23581 aaaaagattt gggaaacagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
    23641 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
    23701 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
    23761 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
    23821 tactgccagg ctggactatt ccgtactggc aagtaccgga tgaagtcaaa ctaccgaaaa
    23881 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
    23941 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
    24001 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
    24061 caccggaata tactctggag caatggctac gttgcgatcc actatggcca catgttttcg
    24121 acgctccagc atatgcacat ctgcaatcga cgctggacgc ggtgcaagta atgcctgatg
    24181 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
    24241 tgacgccgct gttcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
    24301 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
    24361 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
    24421 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
    24481 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcga
    24541 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggagc acgcgcgcat
    24601 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac acgtgacagc
    24661 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtccctggg
    24721 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
    24781 gtaattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg cgatgagttt
    24841 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    24901 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    24961 gatttagtcc gtaacgaaac gttgacctgc tataacggct ttaaaggcga cggttgcggt
    25021 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
    25081 acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg ggtgtcgccg
    25141 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
    25201 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    25261 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
    25321 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
    25381 cgctgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt tatgggccgt
    25441 catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
    25501 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
    25561 ttgtgtttgc atagcgcaat aaccttacgt tgttgttata agtacagagg tcataactgg
    25621 tatgacctct tcagtctggc aaaaaattgc cactatgcaa attaattaca gggttaatac
    25681 cgccagatta cgttccacca gcgaattgcc catccccggc acctgcttta gatcctccac
    25741 cgttttaaac ggaccgtact cttcgcgata actgacaatc gcctgcgctt tcttcaggcc
    25801 aacgccattc atcgcgcggg ctagctcttc cgcgctggca ttattaatgc tgacccgggt
    25861 gccttcttcg tcactggctt tcgccggtac tgctgcttta ctttgtgctg caggagcttc
    25921 cgctttggtt tctaccgccg tcggtttcgc cacagaagct gccgccagcg cgctatgaga
    25981 cattccggca caggccaggg acagggtaat gagcagtgct ttaattccgt gtttcatact
    26041 gttttctcct tgtttgttaa cagtggaatc accttaacgg gagtgaaaac agtaaacaaa
    26101 tggcaatgtt cagaaatgga aaaggccgcg aaagcgacct tttcttgtta cagctgcaac
    26161 gcattttttt gcgaggcggc ttcaggatta ttgctgttcc agcgcatcgc caattttgat
    26221 tttcgcctct ttacgcaggt tactcatcag agcttcaaag acgatttgtg cgttgttctg
    26281 ggtgataccc tgcaccatcg cttttttctg atcttccggc attgaacctt gtttcacttc
    26341 atccagcgcc agcagaacca cattaccttg catatcggtc gccataccgt agctcggttt
    26401 gtctttcgct ggcagtggca gtgcaaacgc cgcctggcta atcgggtcac gaccggaacg
    26461 gcttaagatt ttcggctcgc caaatttcag accggcagcc tgcatagctt ccgcaccttt
    26521 gccggctttc aaatcaacca gcagtttctc agcatccact ttcgcctgtt gttcagcttt
    26581 gttgtgctga accagtgcct taacttgttc ctgaacatct gccaacggtt tcaccgcttc
    26641 cggtttgtgc tcgctgatgc gcagcacgaa tgcgcggtcg ccgtctacgg tgatgatgtc
    26701 agagttgatg cccggcgcgc cgttttcacc taccagaccg ccgttaaaga tagcgtcggc
    26761 aaccggcttg aagttcaact cttccggcag gttatctttg ctaaaccaac ccgtctgagt
    26821 ggctttaacg ccggcagctt gctctgcacc ggccagagac tccgtgtcgt tgcttgccgc
    26881 atcgctcact ttctgctgca gcgcatagta cgcatcgagg gctttttcgt gtttcacttt
    26941 cgccgcaatg tcgtcacgta cttcgtctaa cgatttcact ttcgctggct gaatgtcgtc
    27001 cagacgtaca atcaggaaac cgaccgaaga tttgatgaca ccagacagtt gccctttttc
    27061 tttcagacca gcatttttca gttcgtcggg gatagtggca tcttctaacc aacccatatc
    27121 gccgccgtta cgggcagaga taatatcggc agatttttct ttggctaatg cagcaaaatc
    27181 accgccttta ttcagctcat caagtaccac tttcgcttca tcttcagttt tggtctggat
    27241 gatgctgtag cgggtacgct gcggctgggt gaattgatcc tgatgctgat cgtagtagct
    27301 ctggatatcc gcatcgctaa ccggttgctg catcgttgcg gcatccagct tgatgtaact
    27361 cacgcggaat tgttccggcg tcatgaaatt gtttttgttt tgttcgtagt agctggcaat
    27421 ctcctgttcg gtcacaggct gcttcgccgc cagcgcgtta acatcgatag tcgcctcacg
    27481 caccacgcgt tgttgcgcga ccagtgccgc cagctcgtcg gtttcacctt tcagcataaa
    27541 atcggtaccg gcaacaccgt taatcagctg ttgggtagtg agctggttac gcagcgcctg
    27601 ggcgtactga tcggcggtca tccccatctg gttgaggata ccgttatagc ggctgttatc
    27661 aaatttgccg tcaacctgga aggctggggt cgcgaaaatc gcctgtttaa cctgctcatc
    27721 actgataccc agtttcagct cacgagcgta ctgatccaac agcgcctcgt cgatcagacg
    27781 attcagcacc tgttgacgca gggttttcat atagccttcg ttcgctgcca gctcggagta
    27841 ttgatcgccc agctgttgct gcatgcgatt acgctcgctg ttaaaggcgt tctcgaactg
    27901 cccacggctg atttcctggt cattcacttt tgcggcgtag ttattgcctc cgccaatcag
    27961 gtaaccactc acgccggtca atatgaacga cacgataatg ataccgaaaa taatcttgag
    28021 cacgagactg tttgcagccg tgcgtaagct gtccatcatg gtgtaacaac actccgctgt
    28081 agatgactgt gtacctcacg caacgtatcg atgcgcgcaa aggcctattg tgacaagaaa
    28141 cgggggcaat tgtcagccca caagacacag aaagtcaccg aatacaaata aaaaaggcac
    28201 atcagtagat gcgcccttga acttcgtcac atccccactg gggacaacgc ttagtttacc
    28261 gcgtctttca gtgctttacc tgcacggaag ctcggtactt tagcagcagc gatggtgatc
    28321 tctttaccgg tctgcgggtt gcggccagta cgggcagcac gctctttaac ggcaaaagta
    28381 ccaaaaccta ccagtgctac atcatcccct tctttcagag attcagttac ggaagcaata
    28441 atagcatcta acgcacggcc agccgcagct ttagagatat cagcccctgc agcaatcttg
    28501 tcgatcaatt gagatttatt cactcttctc ttcctcttta taatttatat cgcacttgaa
    28561 tccttcaagg tacgaacgcg cagcagttat atcaggccag ccatcccctt acaacacccc
    28621 ttaataaggg caagcccgat ccgccatcta acttagcgag acaaaaaaag gctggcaagc
    28681 ccgaattagc ctgccagccc tgtttttatt agtgcatttt gcgcgaggtc actattttgc
    28741 agtcacaacc tgcatgccag acggttcatt ttgcagcgcc agagtcagaa cttcctcaat
    28801 gcgcttcaca ggatgaatgt ccagatcggc aattacgttg tcaggaatct cttccagatc
    28861 gcgtttattt tcgaacggaa ttagcactgt tttaatcccg ccgcgatgcg ctgccaggag
    28921 tttttctttc aaaccaccga tcggcagtac ctgaccacgc agagtgatct caccggtcat
    28981 tgccacatcg gcacgaaccg ggttaccggt caggcaagaa accagcgcgg tgcacatagc
    29041 aataccggca ctcggaccat ctttcggcgt cgcaccttcc ggtacgtgga cgtggatgtc
    29101 acgtttttcg taaaaatcag ggttgatccc cagtttttcc gcacgcgcac gaaccaccgt
    29161 taacgccgcc tgaatagact cctgcatcac ttcgccgagc gaaccggtat aggtcagttt
    29221 gcctttaccc ggaacacatg cggtttcaat ggtcagcaag tcaccgccca cttccgtcca
    29281 cgccagaccg gttacctgac cgacacggtt ttcgttatca gcgcgaccat agtcgaaacg
    29341 ctgaacaccg aggtagtcat gcaggttatc gccgttaatt tcgatatgtt ttaatgactt
    29401 atcgagcagt aactgcttaa ccgctttgcg gcacagtttg gagatttcac gctccagacc
    29461 acgcacgccc gcctcacggg tgtagtaacg aataatgccg ataatggcgc tatcgtcgac
    29521 ggtcagctca ccttttttca gtgcattacg ttcaatctgc ttcggcagca ggtgacgttt
    29581 ggcgatgttc agtttttcat cttcggtata accggagagg cgaatcactt ccatacgatc
    29641 cagcagcggt gccggaatgt tcatggagtt cgacgtcgcg acaaacatca cgtcgctgag
    29701 atcgtaatcc acttccaggt agtggtcgct gaacgctacg ttctgctctg gatccagcac
    29761 ttcaagcagt gcagaggccg gatcgcctcg catgtcagaa gacattttgt cgatctcatc
    29821 gagcaggaac agcgggtttt tcacgcccac tttcgccatt ttctggatca gtttacccgg
    29881 catagaaccg atgtaagtac ggcggtgacc acggatttcc gcttcatcac gcacgccgcc
    29941 cagcgccata cggacatatt tacgcccggt ggctttggca atggactgac caagagaggt
    30001 tttacctacc cccggcggcc ctaccaggca gaggatcggt cccttgattt tgttgacacg
    30061 gctttgaacc gcaaggtact caaggatgcg atctttcacg cgctccagac cataatggtc
    30121 ggtatcaagg atttcctgcg cctgacgcag gtcttttttg accttgctgc gcgcattcca
    30181 cggtacctgt accatccagt cgatataacc acgcactacg gtcgcttctg ccgacatcgg
    30241 agacatcatt ttcagcttct gcaactctgc ttccgctttc tcttttgcct ctttcggcat
    30301 tttcgccgcg tcgattttgc gcttcagggc ttcgttttcg tccggcgcgt cgtccatttc
    30361 accgagttct ttctgaatag ctttcatttg ctcgttcaga tagtactcac gctgggattt
    30421 ctccatctgc tttttaacgc ggttgcgaat gcgtttctca acctgcagca gatcgatttc
    30481 cgattccatc attgccatca gatattccag acgttcgtta acgtcggaca tctccagaac
    30541 ggactgtttg tcagccagtt tcagcggcat atgtgcagca atggtatccg ccagacgcgc
    30601 cggatcgtcg atgctattca gcgacgtcag cacttctggt gggatttttt tgttcagctt
    30661 gatgtagcct tcgaactggc tgattgcagt acgcaccagc acttcctgtt cccgctcatc
    30721 aatggtcggc gactccagat actccgcctt cgcagaaaag tgttcgccat tgtcagagag
    30781 cgcagaaata cgcgcgcgct gtaacccctc gaccagcact ttgacggtgc cgtcaggcag
    30841 tttcagcatc tgcaatatag aggccacggt cccgacggtg aaaagatcgt ttacacccgg
    30901 ctcatccgtt gaagcttctt tctgcgcgac cagcataatt tttttatcat ggtccatcgc
    30961 cgcttccaga caacggatag atttttcccg cccgacaaat aaggggatga ccatgtgcgg
    31021 ataaaccacc acatcgcgca gcggcaatac ggggatttca atgcgttcag aacgctcagg
    31081 attcatagag ctctctctta gtttaatttc cgccaggtaa tcagatgaca cgactgtgct
    31141 tcacgccatc tattaacatg tacgtcagta tatggggatg tttcccccac attcaacgcc
    31201 gagaatagag gaaaaattaa aggggagata aaatcccccc tttttggtta actaattgta
    31261 tgggaatggt taattattca ccagatgcct gttgcgcttc cggcttacca taaatcagca
    31321 acggtttgct ttgaccatca attaccgact cgtcgataac cactttttcg acatcttcca
    31381 tggacggcag atcgtacatg gtatcgagca gtgcggcttc tacgatggaa cgcaggccac
    31441 gggcaccggt tttacgcgcc atcgctttct tagcgatagc atccagcgcc tcgtcacgga
    31501 attccagatc cacgccttcc agattaaaca gcgcctgata ctgcttggtc agggcgtttt
    31561 tcggctcttt gaggatctga atcagagctt cttcgctcag ttcattcaac gttgcgacaa
    31621 ccggcagacg accaataaac tcagggataa gaccaaactt gatcagatct tccggttcaa
    31681 cctgcgccag cagctcgcct tcgcttgctt tgtcggactt cgcttttacc gtcgcgccaa
    31741 aaccaatgcc ggagccggtt tctacacggt gggaaatcac tttatccaga ccggcaaacg
    31801 caccgccaca aataaacagg atcttagagg tatcaacctg caagaattcc tgctgcggat
    31861 gtttacgccc accttgcggt ggaacagcag ctaccgtacc ttcgatcagt ttcaacagtg
    31921 cctgctgtac gccttcaccg gaaacgtctc gggtaatgga cgggttgtct gacttacgag
    31981 aaatcttgtc gatctcatcg atgtagacaa tcccgcgctg cgctttctgt acgtcgtaat
    32041 cgcacttctg caacagcttc tgaatgatgt tttcaacgtc ttcgcccaca taaccggctt
    32101 cggtcagcgt ggttgcgtcg gccatggtga acgggacgtc cagcaggcgc gccagcgttt
    32161 cagccagcag cgttttaccg gaaccggtcg gaccgatcag cagaatgtta cttttgccca
    32221 actcgacacc attgctggta tcgccgttgc gcagacgttt gtaatggttg tataccgcga
    32281 ccgccagcac ttttttcgcc tgttcctggc cgataacgta atcgtccagg tggttgcgaa
    32341 tttcatgcgg cgtcggtagc gcactgcgtt cacgatgcgg tgcaacttct ttaatctctt
    32401 cgcgaatgat gtcgttacat aaatcaacac attcgtcgca gatatacacg gatggaccgg
    32461 caatcagctt gcgcacttca tgctggcttt tgccgcaaaa agagcaatac agcaatttgc
    32521 ctgagccatc tttgcgttta tctgtcatgg gtcaaaacct cttctttgtt ctttgtgccg
    32581 cacacgacga cgcaaatgcc attctcaggc gcaagccgct tacagcgttg tgccgccctg
    32641 gataagtata gcggcacagt tgcgcctctg gcatcaatta cgatgggtca gaatcgaatc
    32701 gaccagaccg tattccaccg cttcaggggc ggaaaggaag cgatcgcgct cggtatcacg
    32761 ttcaatctgt tctaatgatt gacccgtatg aagcgccata agttcattca tgcgcccttt
    32821 aactttcaga atttcacggg catgaatttc gatatcggtc gcctggccct ggtagccgcc
    32881 caacggttgg tgaatcatca cgcgcgaatt cggcaggcaa aaacgtttac cttttgcccc
    32941 tgcggtcaac aagaaagcgc ccatcgaggc cgcctggccc atacagatgg tgctgacatc
    33001 aggcttgata aactgcatgg tgtcatagat agacatcccg gcagtgatca ccccgcctgg
    33061 ggagttaatg tacagataga tatctttttc tgggttttcc gcttccagga acagcatctg
    33121 cgccacaatc aggttagcca tgtggtcttc aacctggcca gtcagaaaaa tgacgcgttc
    33181 cttaagtaga cgagaataga tatcaaaaga gcgctcaccg cgtgaggtct gttcaatgac
    33241 catcggcacc agcgccatat ggggtgcaaa gttatctcgt tcgccgctgt atgacatttc
    33301 cgtctcctgg ataaaattga aaaaacctgc tgtaccgatt gtaaccttat ggacggatta
    33361 tcagctagtc cctgtgttat gggtacggca tcaccatatt tcaagcataa caatcttttg
    33421 ttgttacgct aacaccgaaa gaggcttttc gcacggttcc atgcaaaatt catgacaaaa
    33481 aaaaagccca ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt
    33541 aaattacgcc tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc
    33601 tttcgccagt acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat
    33661 cagttctttg tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc
    33721 catctcttcg atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat
    33781 aacttcgccc agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc
    33841 gcgcggcagt tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg
    33901 cagaacgtcg atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac
    33961 cagaccttcg atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc
    34021 catgttttta cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg
    34081 tttgatgaat tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat
    34141 agcgaatttc gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac
    34201 gtcgatggtg aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat
    34261 catacgaccc tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc
    34321 gccgtctaca gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc
    34381 tttttctttc caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc
    34441 gtcggtcact tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac
    34501 ttccggataa acttcaaact ctacagagta agtgaagtct tcacccagct tgtattcgcc
    34561 cggaacataa gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa
    34621 gttacggctc atcaggtcac ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc
    34681 aacgatattc attggcactt tgcctttgcg gaagccgtca atacgtgctt ttttcgcaac
    34741 gttgaccagc tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac
    34801 acggcggcca aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat
    34861 cacagtgctc ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga
    34921 tgttcttata atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg
    34981 acgcggcatt atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg
    35041 tattttttca caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc
    35101 agacaaaaca aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa
    35161 agcgccgaca gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag
    35221 aggcaaagac ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca
    35281 gcgcatgaac ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat
    35341 tcagaaaacg ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag
    35401 agccggctgg gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga
    35461 acgccgccct taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac
    35521 aacgctgaga tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc
    35581 ttaacaacaa atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga
    35641 tttaaagatt gaaacacagg cctcgcatca gcgtttcttt taccaggggt gattatttcg
    35701 tcaacatgat gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg
    35761 gcgggaattt ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa
    35821 tcctcttccc gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta
    35881 ttgaagtttc cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg
    35941 taagcattaa tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata
    36001 atcaaatcgt taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg
    36061 aaaaacagat gaccgcgcgt ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa
    36121 tcatcgttag ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta
    36181 ccaaagcgga tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact
    36241 atcgtgccag ctacaatgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg
    36301 cggttaacag tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcattcacg
    36361 aattcatcaa gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg
    36421 tagacgccca tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg
    36481 ctgatcctgg gttttgcttc cgggctaccg ctcgccctga catccggcac cttacaggcc
    36541 tggatgacgg tcgagaatat tgatctcaaa accattggtt tcttctctct ggtaggccag
    36601 gcttacgttt ttaaattcct ctggtcaccg ctgatggacc gctacacgcc cccatttttt
    36661 gggcggcggc gcggttggct gctcgccacg caaatcctgt tattagtcgc cattgcggcg
    36721 atgggttttc tcgaacctgg cacccaactc cgctggatgg cggcactggc ggtggtgatc
    36781 gctttttgct cggcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg
    36841 gcagaagaac gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg
    36901 gtttccggcg gcttagccct gtggctggca gataaatggc tgggctggca gggcatgtac
    36961 tggttgatgg cggcgctgtt gatcccttgt attatcgcga cgttgcttgc accagaaccg
    37021 accgacacca ttcctgtgcc aaaaacgctg gaacaagcgg ttgttgcacc tctgcgagat
    37081 ttctttggtc gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc
    37141 gacgcattcg ccatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg
    37201 ggtgaagtag gcgtggttaa caaaacgctt ggcttactgg cgaccattgt gggcgcattg
    37261 tacggtggga ttttgatgca gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt
    37321 ttacaaggtg cgtctaacgc tggttactgg ctgctgtcga ttactgataa gcatctctac
    37381 agcatgggcg cagccgtctt tttcgaaaac ctctgtggcg ggatgggcac atcagccttt
    37441 gtcgcgctgt taatgacgct gtgtaataag tcattttccg caactcaatt tgccctgctc
    37501 tcagcgcttt ccgctgtagg acgagtttat gtcggccccg tggcgggttg gtttgttgaa
    37561 gcacacggct ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc ggggcttatt
    37621 ttgctgctgg tttgccgcca gacgcttgaa tatacaggag taaatgacaa ctttatctcc
    37681 cgtaccgaat atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc
    37741 ctgttggccg tgtggttact gctgttgacg atggacgcgc tggatttgac gcacttctct
    37801 ttcctgcctg ctctgctgga agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt
    37861 ggtttgctgg attatctggc gctacgaaaa acgcatctga cgtaatctgt aaatattatt
    37921 tagctgtagt aatcactcgc cgtaattatt acggctaaat aaacatcagt atcgcttaat
    37981 aatatttgtt cttttgtgcg gcttagcgtt tggaaattat tgacgcaatt tatgaattct
    38041 attttttctt acacgattca gctaatgagt ctttattttc ccccacccaa ttgtcaccat
    38101 aatgataatt atttgttaaa caattgtttt attccagatt ggttatacca attgcccgtc
    38161 caaactcctt ctgcactccc ctttcgttat aacgcccttt tgcaacagct tcttaaaatc
    38221 aacttgatat gttttgcaac atatgtgacc tgacagccaa atccaagtaa caggaattta
    38281 atcatgttta cagtaatgta accttcccgt aaaatgccca cacactttaa acgccaccag
    38341 atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg
    38401 ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc
    38461 aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg
    38521 attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta tcgtgcgagc
    38581 aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc
    38641 tggacggtac ctatcttaat catcatcttc cttgcggtac tgacctggaa aaccactcac
    38701 gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt
    38761 tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgctac cgtgaatgaa
    38821 atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac
    38881 tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc
    38941 ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggg
    39001 ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgaattcgac
    39061 caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc tgcgttcgaa
    39121 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
    39181 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
    39241 ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag ccacgcggaa
    39301 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
    39361 gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
    39421 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
    39481 acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg
    39541 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggc
    39601 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
    39661 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
    39721 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact
    39781 gttgttggtg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
    39841 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgtcgattac
    39901 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
    39961 gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc
    40021 tgggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc
    40081 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
    40141 ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc
    40201 ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
    40261 ctgtttggtt atacctcgct ggtatgggca accgtctgta tcaccgtgct gtcgttcatc
    40321 gtttggctgc accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt
    40381 atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
    40441 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggaccatcgg ttttatcgtc
    40501 actttctcgg tgggcgggat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
    40561 ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc
    40621 ttcggctgct tcgcagggat gacctactgg tggcctaaag ccttcggttt caaactgaat
    40681 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
    40741 ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
    40801 cagttccaca ccatgctgat gattgcagct agcggtgcag tactgattgc gctgggtatt
    40861 ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa ccgtgacctg
    40921 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
    40981 tataactttg ccgttgtgcc gcacgttcac gagcgtgatg cattctggga aatgaaagag
    41041 aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
    41101 ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat
    41161 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
    41221 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
    41281 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
    41341 gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
    41401 ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta cctatgccgt
    41461 tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt
    41521 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
    41581 catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg
    41641 tgccggattt atcgggatgg aaatctacga attccatcac ctgattgtta acggcatggg
    41701 tccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca
    41761 cgtcacttcc ggtcttatct ggatggcggt gctgatggta caaatcgctc gtcgcggcct
    41821 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
    41881 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
    41941 accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg
    42001 tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc
    42061 gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc
    42121 ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc
    42181 gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac
    42241 aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga
    42301 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa
    42361 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
    42421 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
    42481 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
    42541 ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
    42601 ggctgggggt gatgggcttt gtggtttatg tcggcgtcta tagcctgtac atgaaacgcc
    42661 actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggct
    42721 actgtgcagt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc
    42781 tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg
    42841 caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc
    42901 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctggat
    42961 ataaatatct ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg gctctgcgcg
    43021 gttataaagt tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg
    43081 ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtaccggac tcgcatacgc
    43141 tgctggctgc tgtgtggtaa caaaacctct ctattaaaaa ggtgctacgg cacctttttt
    43201 attgtctgag atcagagata cggctaataa ccgtatgtct tgacagagtg agcagatgaa
    43261 cataaattaa tcatacggtt gatagccgta ttatgtatga gatcgactcc ctacgatttc
    43321 attcagttaa tcaaagaggt cataatgcct ggaaaaacag ccactctacc cgatgtcgat
    43381 aaaacgctga aaaatgcgcg ggtcgaactc aaaactagtc ccgacgccaa aaacaaactt
    43441 cgtgaagctg cgcaagccgt tggggtagat ttaagcgctt ttattttgag cgctgctatg
    43501 gaacgcgccg aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa tcaaagctgg
    43561 gaactgatga accaactcat cgctgaacct gctcaaccga cgctcgccct caaggcgtta
    43621 atgaaaagga aaaacagcga tggtcgacaa gcatgaagag attactctgc ccatagtcct
    43681 ctcctgtaat tatcagtctg atattactta tcctgggcaa aaacagtttg attgcggtaa
    43741 ccctgttatc gataaatttg tacgcgcatc gctaaagaaa agtgtgcgta atagcgactg
    43801 tgcggctaaa gcacttattg acagacaaag tggtgaactg atcggcatct gtacttttac
    43861 ggcatattcg ctggaaaaac aacgcgtttc tggcgtcctt cagggttcac aaccttcaga
    43921 aattggtgtt gtcagattag tcatgttggg ggtagcacgg aagtatcaaa agcggggctt
    43981 tggtcaggac ctactatgtg atttttttga acatgtaaaa ataattcacc aggcattacc
    44041 aattaaaggg gtttatcttg atgctgaccc tgccgccatt aatttttatg ctcgtctcgg
    44101 ctttgttcag ctttcagcga caccaaatgc ttttggtgct gtacctatgt ttttggcgat
    44161 tcagcatatt ctcgcggctt agattattat cgccacccgc cagggcaagt tttgcctcag
    44221 ccgggggctg agcagcgtta ccacttttgt tcaattgccc ccgctttcag caactccaca
    44281 tccttgccgt taatggttgc cgttgcggca acgattgtgg tccttacagt tgcacaagat
    44341 tttcgctcaa attccggttt ataaagaata agagacgaat tactggtaaa tgaaccaaat
    44401 aggtaatcag taaagctaat attcagccgg tttaaaccga aaggcgcgaa gccaaccgtt
    44461 tggttaatct ccttaccgga tgggcgacga atgctgaatg tcacgcgaag attggccact
    44521 tcgcctaatt tattggtggt ggcgaagagg ctatccatgc aggaaaaacc tttatctaaa
    44581 aaattatctc gcatcccaga acgggtaacc gtaatcacgg gtaacgtatc gtcaaccgtt
    44641 tttctgtcaa tcatgtcttt tcgacaagta gccagaaagt cttctgggcc accataaatg
    44701 tggacacctt tatcaagata ttccttcgct ttcttacaat ctacctcaac atactgacca
    44761 ttggcgtaca taatgcccaa cctggacctt gctaaattca caccgtgctg cgcggcctga
    44821 ttaaatagtg catacgcttt ggcataatcc acccgcacct gctcaccgtc gtagtagata
    44881 ccccccagcg ctaacggagc ttctctgtca ccttgtttag ccactaactc gaaccattta
    44941 atcgcttcct gaacatcagg tttggtatca taacgaggct gataatattc ccagcctaac
    45001 aggctctgcg cgtcgatatc tcccgattca gccattttat gcagtgtatt tataacatcg
    45061 ggagcaggtt taccaccgta tatgctgtca tacaattcat gcgctttctc gctggccacc
    45121 acgcttggtg aaactatcag cattacagaa aataatattc ttttagttaa catgtatacc
    45181 tttatgtcct cactgtcata tatttccata ataattaacc accaaatatc catttaatga
    45241 ataaatacag tgccataaag gtccaagtaa taagcacaca tttagcctga aacattatca
    45301 tccatgtcac caaatcgttt atttagtgag ataaaactag catattcgta agataaaaaa
    45361 tgcacatagc aatcaagaaa tgatattatt acttttttga tgtaaaacta tttggtttta
    45421 aaattcattt tttcaacata tagagcattc attttatgaa tgcaaaaaaa catacctgac
    45481 acacaaatta ctctcctctg taatattttc tcattaacac tctgtttacc caacatgcgc
    45541 tccgcactac actatgtggg cattttttta aggggtggta atgaacgatt ataaaatgac
    45601 gccaggtgag aggcgcgcga cctggggttt agggaccgta ttctcgttgc gcatgctggg
    45661 catgtttatg gttctgccgg ttctgaccac gtacggcatg gctctgcaag gtgccagcga
    45721 agcattaatc ggtattgcca ttggtattta tggtctgact caggccgttt ttcagattcc
    45781 gtttggcctg ctttccgacc gcattggtcg caaaccatta attgtcggtg ggctggcggt
    45841 gtttgccgcc ggtagcgtta tcgctgcgct ttccgactcc atctggggaa ttattctggg
    45901 ccgggcgcta caaggctctg gagcaattgc cgccgccgtt atggcgctgc tttccgatct
    45961 cacgcgcgaa caaaaccgca ccaaagcgat ggcgtttatc ggcgtgagct ttggcattac
    46021 ctttgccatt gcgatggtgc ttggcccgat catcactcac aaacttgggc tgcacgcgct
    46081 gttctggatg atcgctattc tggcaacaac cggcattgcg ttgaccattt gggttgtacc
    46141 caacagtagc actcacgtac ttaatcgtga gtccggaatg gtgaaaggca gtttcagtaa
    46201 agtgctggcg gaaccgcggc tgctaaaact caactttggc attatgtgtc tgcacatgct
    46261 tctgatgtct acgtttgttg ccctgcccgg acagctagct gatgcggggt tcccggcggc
    46321 tgaacactgg aaggtctatc tggcgacgat gttaatcgcc tttggctcgg tcgtgccttt
    46381 cattatctac gctgaagtta agcgcaaaat gaagcaagtc tttgtcttct gcgtcgggtt
    46441 gatcgtggtt gcggaaattg tgttgtggaa cgcacaaacg cagttctggc aactggtggt
    46501 cggcgtgcag cttttctttg tggcgtttaa tttgatggaa gcccttctgc cctcacttat
    46561 cagtaaagag tcgccagcag gttacaaagg tacggcgatg ggtgtctact ccaccagcca
    46621 gtttcttggc gtggcgattg gcggttcgct gggcggctgg attgacggca tgtttgacgg
    46681 tcagggggta tttctcgctg gcgcaatgct ggccgcagtg tggctggcag tcgccagtac
    46741 catgaaagaa ccgccgtatg tcagcagttt gcgcattgaa atcccggcgg acattgccgc
    46801 aaacgaagcg ttaaaagtgc gtttgctgga aactgaaggc gtcaaagaag tgttgattgc
    46861 agaagaagaa cattcagctt atgtgaaaat cgacagcaaa gtgacgaatc gctttgagat
    46921 agaacaggca attcgtcagg cataaaaaac gcggcgaatt gcctgctgcg ctacgcttat
    46981 caggcctaca aagtttctga attacaaacc tttgtaggcc gaatcaggcg ttcatgccgc
    47041 atctgacatg aacaaaacgc acatagtcgc gattaatcgc ggaagttttt gaactggaac
    47101 ggctgaccga gatcgccacc acgtaccatc gccatgacag cctgcaaatc atcacgagat
    47161 ttgcccgtta cgcggatttc atcgccctga atttgcgcct gcactttcag tttgctgtct
    47221 ttgatcatct tgacgatttt cttctgggtc gcgctctcaa tgccctgttt cagtttcgct
    47281 tccacaaacc aggttttacc gctatgaacg atattctccg gtacatccag cgaactgcct
    47341 tcaatgccgc gcttcagcag cttggcacgc agaatatcca gcaactgatt gacctggaaa
    47401 tcggactcgc tcaacacttt gatggttttg ctggcgtcgt tcagctcaaa tgaggcttca
    47461 acgttacgga agtcaaaacg ggactccact tcgcggctcg cgttatcgac cgcgttacgt
    47521 gcttcctgaa gatcaacttc agagacaata tcgaaagatg gcatcttttc ctctcccttc
    47581 atttttgatg cgaagcataa tacccgcaaa gttaaataac cgacagtcct gcaccaggac
    47641 gtatccctta attgtgcata gtcgcaacag catgcacaat taagggatag ccttattgaa
    47701 cacccggagt ggttgcgggt gaggaggaac aatgaaaatt accgtattgg gatgcggtgc
    47761 cttagggcaa ttatggctta cagcactttg caaacagggt catgaagttc agggctggct
    47821 gcgcgtaccg caaccttatt gtagcgtgaa tctggttgag acagatggtt cgatatttaa
    47881 cgaatcgctg accgccaacg atcccgattt tcttgccacc agcgatctgc tcctggtgac
    47941 gctgaaagca tggcaggttt acgatgccgt caaaagcctc gcgtccacac tgcctgtaac
    48001 cacgccaata ctgttaattc acaacggcat gggcaccatc gaagagttgc aaaacattca
    48061 gcagccatta ctgatgggca ccaccaccca tgccgcccgc cgcgacggca atgtcattat
    48121 tcatgtggca aacggaatca cgcatattgg tccggcacgg caacaggatg gcgattacag
    48181 ttatctggcg gatattttgc aaaccgtgtt gcctgacgtt gcctggcata acaatattcg
    48241 cgccgagctg tggcgcaagc tggccgtcaa ctgtgtgatt aatccactga ccgccatctg
    48301 gaattgcccg aatggtgaat tacgtcatca tccgcaagaa attatgcaga tatgcgaaga
    48361 agtcgcggca gtgatcgagc gcgaagggca tcatacttca gcagaagatt tgcgtgatta
    48421 cgtgatgcag gtgattgatg ccacagcgga aaatatctcg tcgatgttgc aggatatccg
    48481 cgcgctgcgc cacaccgaaa tcgactatat caatggtttt ctcttacgcc gcgcccgcgc
    48541 gcatgggatt gccgtaccgg aaaacacccg cctgtttgaa atggtaaaaa gaaaggagag
    48601 tgaatatgag cgcatcggca ctggtttgcc tcgcccctgg tagtgaagag actgaagccg
    48661 tcaccactat cgatctgctg gttcgcggcg gtatcaaagt caccactgcc agcgtcgcca
    48721 gcgatggcaa cctggccatt acctgctcgc gcggcgtgaa gctgctggcg gatgcgccgc
    48781 tggtcgaggt ggctgatggc gaatatgacg tgatcgtact gcctggtggc attaaaggcg
    48841 cagagtgttt tcgcgatagt accctgctgg ttgaaaccgt taaacagttc caccgttccg
    48901 ggcgtatcgt cgcggctatt tgcgccgcgc cagccaccgt gctggtgccg cacgatatct
    48961 tcccgattgg taatatgacc ggcttcccga cgctgaaaga caaaattccc gctgaacaat
    49021 ggcaggacaa gcgcgtcgtc tgggatgcac gggtaaaatt gctgaccagc caggggccgg
    49081 ggacagctat cgactttggt ctgaaaatta tcgacctgct ggttgggcgc gagaaagccc
    49141 atgaagtggc atcacaactg gtgatggcgg cagggattta taattattac gagtagtgtc
    49201 ggatgcggta agcgtcgcat ctgaccagat gcgacgttac aaccacaaat tacgggcgat
    49261 ataccttcac attgttaaag ccctgctcgc gcagatagag cgcctgcaga cggctcatca
    49321 ccccgcgctc acaccacagt agccaggttc tgttctggtc gagatcgcca aacttggtgc
    49381 tcagtttata gaacggcaga gaaactacat caatcccttc gactttcagt ggcttatctt
    49441 cctgttcatc gatagaacgg atatcgagga tcacgtcgtt cgggccgaag ccattgacgg
    49501 tttccacttc caccacttcc tgctcggtct gctgggcgat ttcgcggata tcaacgttat
    49561 tcgcttcttc aaccacttta tcgagaatgc tgaagtcgaa tttctcttct tccgcttcaa
    49621 tcttcgactt aaccgctttc accgtcgggc ttttggagat aacaccgcaa tattccggca
    49681 tcgtgcgagc aaagtcttcg gtgccaatct ggcgagccag gttgatgatg tgctctttgt
    49741 cgtaagaaat cagcggacgc aggatcagcg tatcggagac gttatcaatc aggcgcaggt
    49801 tggtcagcgt ctgactggac acctggccga gcgcttcgcc ggtgaccagc gcctgtacgc
    49861 cgtaacgttc agccacttta gacgcggcac gcaccatcat acgtttgagg ataacgccca
    49921 tctgaccgtc gtcgattttc tcgagaattt ccccgacgac cggttcgaaa ttaatagcga
    49981 caaaacgcac gcggtgggag ctgccaaaac ggttccacag ataatgcgcc acctgacgca
    50041 cgccaatttc atgcgccgcg ccgccaaggt taaagaaaca gtaatgcacg cggcagccgc
    50101 gacgcatcaa catataactg gaaacaccgg agtcgaaacc accggaaatg agcgacagca
    50161 catcttcctg ggtgccgatc gggaaaccgc caataccttc gtagcggcct ttaatcagca
    50221 ggagacgatc gtcttccact tccagatgga cagtcacttc cggattggtc agcttcacgc
    50281 gcgcggactc aatatgctga tttaaaccgc cgccgacgta acgttccaca tcaatcgagc
    50341 taaaatcatg tttgccacgg cgcttcacgc gtacgcagaa ggttttgcct tccagctgat
    50401 cgcgatactg aaccaacgct ttctcaaaaa tatcgtgcat gtcggtaaac ggcacgtctt
    50461 cgacttcgag aatatggtgg atacccggaa tacgggtcag ggcgtcgcga atagccagac
    50521 gctggttttc atcttttgcg cgaacttcga tgttatccca gtggcggacg acagcgagcg
    50581 tctcatcata gtgctttaaa acgttacgaa tgttcccggt aaggattttt ataaagcgca
    50641 agcgcacaga ttggcttttg atggtgattt ccgggaacaa tttaatgata aacttcatgg
    50701 cggcaatggt tcgttggcaa gccttaagcg acttgtatag ggaaaaatac agcagcccac
    50761 acctgcggct gcatccaggc gcggaagtat accactaaca tcgctttgct gcgcacatca
    50821 ccttaccatt gcgcgttatt tgctatttgc cctgagtccg ttaccatgac ggggcgaaaa
    50881 atattgagag tcagacattc attatgccga agaaaaatga ggctcccgcc agctttgaaa
    50941 aggcgctgag cgagctggaa cagattgtaa cccgtctgga aagtggcgac ctgccgctgg
    51001 aagaggcgct gaacgagttc gaacgcggcg tgcagctggc acgtcagggg caggccaaat
    51061 tacaacaagc cgaacagcgc gtacaaattc tgctgtctga caatgaagac gcctctctaa
    51121 ccccttttac accggacaat gagtaatgga ctttccgcag caactcgaag cctgcgttaa
    51181 gcaggccaac caggcgctga gccgttttat cgccccactg ccctttcaga acactcccgt
    51241 ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag cgtctgcgac ccttcctggt
    51301 ttatgccacc ggtcatatgt ttggcgttag cacaaacacg ctggacgcac ccgctgccgc
    51361 cgtagagtgt atccacgctt actcattaat tcatgatgat ttaccggcga tggatgatga
    51421 cgatctgcgt cgcggtttgc cgacctgcca tgtgaagttt ggcgaagcaa acgcgattct
    51481 cgctggcgac gctttacaaa cgctggcgtt ctcgattcta agcgatgcca atatgccgga
    51541 agtgtcggat cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca gcggtattgc
    51601 cggaatgtgc ggtggtcagg cattagattt agacgcggaa ggcaaacacg tacctctgga
    51661 cgcgcttgag cgtattcatc gtcataaaac cggcgcattg attcgcgccg ccgttcgcct
    51721 tggtgcatta agcgccggag ataaagggcg tcgtgctctg ccagtactcg acaagtacgc
    51781 agagagcatc ggccttgcct tccaggttca ggatgacatc ctggatgtgg taggagatac
    51841 tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta cctaccctgc
    51901 acttctgggt cttgagcaag cccggaagaa agcccgggat ctgatcgacg atgcccgtca
    51961 gtcgctgaaa caactggctg aacaatcact cgatacctcg gcactggaag cgctagcgga
    52021 ctacatcatc cagcgtaata aataaacaat aagtattaat aggcccctga tgagttttga
    52081 tattgccaaa tacccgaccc tggcactggt cgactccacc caggagttac gactgttgcc
    52141 gaaagagagt ttaccgaaac tctgcgacga actgcgccgc tatttactcg acagcgtgag
    52201 ccgttccagc gggcacttcg cctccgggct gggcacggtc gaactgaccg tggcgctgca
    52261 ctatgtctac aacaccccgt ttgaccaatt gatttgggat gtggggcatc aggcttatcc
    52321 gcataaaatt ttgaccggac gccgcgacaa aatcggcacc atccgtcaga aaggcggcct
    52381 gcacccgttc ccgtggcgcg gcgaaagcga atatgacgta ttaagcgtcg ggcattcatc
    52441 aacctccatc agtgccggaa ttggtattgc ggttgctgcc gagaaagaag gcaaaaatcg
    52501 ccgtaccgtc tgtgtaatcg gcgatggcgc gattaccgca ggcatggcgt ttgaagcgat
    52561 gaatcacgcg ggcgatatcc gtcctgatat gctggtggtc ctcaacgaca acgaaatgtc
    52621 gatttccgaa aatgtcggcg cgctcaacaa ccatctggcg cagctgcttt ccggtaagct
    52681 ttactcttca ctgcgcgaag gcgggaaaaa agttttctct ggcgttccgc caattaaaga
    52741 gctgctcaaa cgtaccgaag aacatattaa aggcatggta gtgcctggca cgttgtttga
    52801 agagctgggc tttaactaca tcggcccggt tgacggtcac gatgtgctgg ggcttatcac
    52861 cacgctgaag aacatgcgcg acctgaaagg cccgcagttc ctgcatatca tgaccaaaaa
    52921 aggtcgtggt tatgaaccgg cagaaaaaga ccccatcact ttccacgccg tgcctaaatt
    52981 tgatccctcc agcggttgtt tgccgaaaag tagcggcggt ttgccgagct attcaaaaat
    53041 ctttggcgac tggttgtgcg aaacggcagc gaaagacaac aagctgatgg cgattactcc
    53101 ggcgatgcgt gaaggttccg gcatggtcga gttttcacgt aaattcccgg atcgttactt
    53161 cgacgtggca atcgccgagc aacacgcggt gacctttgcc gccggtctgg cgattggtgg
    53221 gtacaaaccc attgtcgcga tttactccac tttcctgcaa cgcgcctatg atcaggtgct
    53281 gcatgacgtg gcgattcaaa agcttccggt cctgttcgcc atcgaccgcg cgggcattgt
    53341 tggtgctgac ggtcaaaccc atcagggcgc ttttgacctc tcttacctgc gctgcatacc
    53401 ggaaatggtc attatgaccc cgagcgatga aaacgaatgt cgccagatgc tctataccgg
    53461 ctatcactat aacgacggcc cgtccgcggt gcgctacccg cgcggtaacg cggttggcgt
    53521 ggaactgacg ccgctggaaa aactgccaat tggcaaaggc attgtgaagc gtcgtggcga
    53581 gaaactggcg atccttaact ttggtacgct gatgccagac gcggcgaaag tcgctgaatc
    53641 gctgaacgct acgctggtcg atatgcgttt tgtgaaaccg cttgatgaag cgttaattct
    53701 ggaaatggcc gccagccatg aagcgctggt caccgtagaa gaaaacgcca ttatgggcgg
    53761 cgcaggcagc ggcgtgaacg aagtgctaat ggcccatcgt aaaccagtac ccgtgctgaa
    53821 cattggcctg cctgacttct ttattccaca aggaactcag gaagaaatgc gcgccgaact
    53881 cggcctcgat gccgccggta tggaagccaa aatcaaggcc tggctggcat aatccctact
    53941 ccactcctgc tatgcttaag aaattattca tagactctaa ataattcgag ttgcaggaag
    54001 gcggcaaacg agtgaagccc caggagctta cataagtaag tgactggggt gaacgaatgc
    54061 agccgcagca catgcaactt gaagtatgac gagtatagcg ggagtggcag catgcaatac
    54121 aaccccttag gaaaaaccga ccttcgcgtt tcccgacttt gcctcggctg tatgaccttt
    54181 ggcgagccag atcgcggtaa tcacgcatgg acactgccgg aagagagcag ccgtcccatc
    54241 attaaacgcg cgctggaagg cggcataaat ttctttgata ccgccaatag ctattccgat
    54301 ggcagcagcg aagagatcgt tggtcgcgca ctgcgggatt tcgcccgtcg tgaagacgtg
    54361 gtcgttgcga cgaaagtgtt ccatcgcgtt ggtgatttac cggaaggatt atcccgtgca
    54421 caaattttgc gctctatcga cgacagcctg cgccgtctcg gcatggatta tgtcgatatc
    54481 ctgcaaattc atcgctggga ttacaacacg ccgatcgaag agacgctgga agccctgaac
    54541 gacgtggtaa aagccgggaa agcgcgttat atcggcgcgt catccatgca cgcttcgcag
    54601 tttgctcagg ccctgaagct acaaaaacag cacggctggg cgcagtttgt cagtatgcag
    54661 gatcactaca atctgattta tcgcgaagaa gagagcgaga tgctgccact gtgttatcag
    54721 gagggcgtgg cggtgattcc gtggagcccg ctggcgcggg ggcgactgac gcgtccgtgg
    54781 ggagaaacta ccgcacgact ggtgtctgat gaggtgggga aaaatctcta taaagaaagc
    54841 gatgaaaatg acgcgcagat cgcagagcgg ttaacaggcg tcagcgaaga actcggtgca
    54901 acacgagcac aagttgcgct ggcctggttg ttgagtaaac cgggcattgc cgcaccgatt
    54961 atcggaactt cgcgcgaaga acagcttgat gagctattga acgcggtgga tatcactttg
    55021 aagccggaac agattgccga actggaaacg ccgtataaac cgcatgcggt cgtaggattt
    55081 aaataagagt ccaggcctga tgagacgtga caagcgtcac atcaggcatc ggtgcacaac
    55141 tacgacagaa tacccagcgg ccagtgatga ccgataaagt acaggatgcc tgcggaaatc
    55201 accccggcga caatatcgtc gatcatgatc cccatgccgc catgtacatt gcgatcaaac
    55261 cagcggatcg gccacggctt ccacatatcc agaatacgga aaatcacaaa cccggcggca
    55321 acccactgcc agtcattggt cggcagcgcc atgagcgtga tccacatacc aataaattcg
    55381 tcccagacaa tgctgccgtg atcgtgcacg cccatatctt tcgccgtttg atgacaaaga
    55441 tagacgccga tacagatccc caacatcacc accagcgagt agagctgcca gggtaaaaag
    55501 gtcatcagat accagaacgg aatcgctgcc agcgatccca tcgtgccagg aacgattggg
    55561 cttaatccac ttccgaatcc gacagcaagc agatgccacg gattactcat cttcaggcga
    55621 cttttcgcga catctttatg gcgtggcaaa atggtcatat cctttccagt caaacgtgac
    55681 aggttcgccg tcacgaataa aacaaagccc ttcgatatcg gcggtcattt gcccgataca
    55741 ggtaaacggt acgcccaggt gtccgagagc cacatccagc gcgccacggt tcagttccgg
    55801 cacagtgaaa cacaactcgt aatcttcacc gccagagagc gcccagcgca gcgcctgttc
    55861 cggttcaaca tggcgagaaa gcgcatcaga aaacggcagc aatgccaggt caatacgtgc
    55921 gccgcagtcg ctggctttca cgatatgccc gagatcggaa atcagaccgt cagagagatc
    55981 gatagctgaa tttgccagat cgcgcagtgc ctgcccctgt aaaatacgcg gcgatggacg
    56041 gagatgacgt ttgatcaagt agtcagcatc tttagcatcg gcaacctgca aacggttttg
    56101 caaaatcgcc agcccggcgg cgctatcgcc cggtgtaccg gtcacataga tccagtcacc
    56161 cggtttcgcc ccagagcgcg ttaaggctcg tcccatcgga acaaagccgt ggatacccaa
    56221 cgtcattgat aatggcccac gcgtggtatc gccgccaatg agttgcatat cgtaataatt
    56281 gagaagatca aacaaactgt cgctgaagga ctcaagccac gcttcgtcta cgtccggtaa
    56341 ggttaatgcc agcgtcagcc aggccggatc ggcccccatc gctgccagat cgcttaggtt
    56401 cactgccagt gctttatacg ccagatcagc aggatcgata tcatggagga agtggttgcc
    56461 cgccaccagc gtatcagtgc tgatcgccag ggtctgtttc tcggggatat tgagaagagc
    56521 gcaatcgtcg ccgatgccca gttcgacatc aagacgagaa cttcttacac ggtcaaaata
    56581 acgggcaatc agggagaact cgccacatgc catacgttat gcctcagcag gtaaaaaaga
    56641 aaaggccggc gatcgcggaa cggtcttccg tgaatctacc ggcctggata tcactttttg
    56701 ttagggcgaa tcacaggtgc tgctttatcg agtacgccgt tgacgaactt atggctgtct
    56761 tctgcgccga acgatttcgc cagttcgatc gcttcgttaa tggccacttt gtatggcaca
    56821 tcgctacgtt tagacagctc gtacagcgca atgcgcagta ctgctttttc tacctgtccc
    56881 agttcttcca gcaggcggga caggtatggc ttcatcagtc cgtcgaggta tgcggtgtta
    56941 gtcgccaccc cggccagcag ctcacggaag tacaggacgt caacgtcttt tacatcctgt
    57001 tcagccagga actggtattc aacatcagcg atgtcgttct gggacaactg ccaggagtag
    57061 agcgcctgga cggcacactc acgagcgcgg cgacgagcag caggtttcac ggatttcccc
    57121 ttactaattt caggccttga tggctttcaa tacattaatc atttcaagcg cggtcagtgc
    57181 agcttctgca cctttgttgc cagctttggt gccagcacgt tcgatcgctt gttcaatgct
    57241 ttcagtggtc agaaccccaa aagcaaccgg aatttcgctg tcctgggcaa catgcgccag
    57301 gccgttgctt gcaccaccag cgacatattc aaagtgggca gtgccaccac gaataaccgt
    57361 acccagcgca atcaccgcgt cgtatttacc ggttttagcc agtgcacccg ccgccagcgg
    57421 cagctcatag gcaccaggca cccaaacaac ggtaatgttt tcatctttta cctgaccgat
    57481 acgtttcagt gcgtcaattg caccttccag caggctgtca ttgataaagt tgttgaaacg
    57541 cgcgatggtg atggcgacgc gagcgtccgg ggtagcaacg ttagcttcaa taatgttcat
    57601 tttcttcctt cgggttcgag tatggccccg caggggggcg gattttagca taatatttcg
    57661 tgcgctgctt ccctttcgag ccgggagatc atgcacccac taaatgcagg caaacatccg
    57721 ggcctacatg acgtatctct ttgaatttaa attggggggc gtcggctaat ttctcaagcc
    57781 ctggcagcgt gcataatccg cgggcgtcgc tgcctaatag tttaggtgcg atatagacaa
    57841 tcagctcatc gactaaaccc gcctgcagca atgcgccagc gagcgttggc cccgcttcca
    57901 cccagatact gttaatttgc tgtttaccca gttgcatcat cagtacaacc agatccagat
    57961 gacctttatg ctctggaatc agcaaggtac gcaccgtttc cggccactca ctagaatctt
    58021 cctgcgtacg cgcgaaccag gtttcgccgg gctgctgcac aatgcgatgt tccggcgtca
    58081 cgcgattttg gctatcaatc acaatacgta ctggctggcg gagattttgt tgcgggtaga
    58141 gcgcctgagt ttgttcatcc agttcagacc aacgcactgt taaggctgga tcatccgcca
    58201 gcaccgtggc gctgctagtt aaaatggcat gactttgtgc gcgctgtcgt tgtacgtcgc
    58261 gccgcgcctg aggcgaagtg atccactggc tttcgccgct cgccatcgcc gtgcggccat
    58321 caagcgatgc gccaagtttt aactgaatat aaggaaagcc ggtgcgcatc cgcttgagaa
    58381 agcctttatt caattgctcg gcttcactca tcatcagccc gtggctgacg tcaatgccag
    58441 cctgttgcag acggtaaagt ccacgcccag cgacctgcgg gttaggatct tgcatcgagg
    58501 caaccacgcg cgctacgcca gcggcgatga gtgcgtcaca gcacggtggc gtacgaccat
    58561 gatggctaca gggttcgagt gtgacatagg cggtcgcacc tttggctttt tcacccgcca
    58621 tacgcaacgc gtgtacttcg gcatgtggtt cacccgcacg ttggtggtaa ccttcaccga
    58681 caatttcgcc atctttgaca atgacgcacc cgacattcgg gttgggatgc gtggtaaaac
    58741 gtcctcgttg cgccagcttt agcgctcgcg ccatgtaata ctcgtcctgc acggcttagt
    58801 cctccaggcg cgcgatctct tcgccaaatt ctttgatatc ttcgaaactg cggtagacag
    58861 aggcaaaacg gatataggcg actttatcga gctttttcaa ttgctccatc accagattgc
    58921 caatcatctt gctcggcact tcgcgctcac cggtggcgcg cagctgcgat ttaatatgat
    58981 tgattgccat ttcgacgtca tcggaactca ccggacgttt ttccagcgcc cgcagcattc
    59041 cgctacgcaa tttctcttca ttaaacggtt cacgcacgtc gttgcttttt acaacacgcg
    59101 gcataaccag ctccgccact tcaaaggtgg tgaaacgttc attacacacc agacactgcc
    59161 gacggcggcg tacggatgaa ccctcgccca cgagacgaga gtcaattacc ttagtgtcca
    59221 cggcgaaaca gaatgggcaa tgcatacggc gtcctgacca ggtggttaac agaaatctat
    59281 tttaccctga actgacgata caacaaaggc gcaacgcttt tgagataaag gatcgatgct
    59341 ttcgctgctt ttaccctcta ccattaggct aattgcgacg agaaagggac gacctatgaa
    59401 tactaacgtt tttcgactgc tcctgctggg aagcctgttc agccttagcg cctgtgtgca
    59461 gcagagcgaa gtgcgacaga tgaaacacag cgtcagcacg ctgaaccaag agatgacgca
    59521 actcaataaa gaaacggtca aaatcactca gcaaaacagg ctgaatgcaa aatccagcag
    59581 tggggtttac cttctgccag gagcgaaaac accggcaaga ctggaaagcc agatcggtac
    59641 tttacgtatg tcgctggtga atattacgcc tgatgcagat ggcaccaccc tgacgctacg
    59701 tattcagggc gagtcgaatg atcccttgcc cgcattcagc ggaactgttg aatatgggca
    59761 aattcaggga acaatagaca actttcagga aatcaatgtg cagaatcaat tgattaatgc
    59821 ccctgccagc gtcctcgccc ccagcgatgt tgatattccg ttacagttaa agggtatttc
    59881 tgtagaacag ttagacttcg tacgtatcca cgatattcaa cctgttatgc agtaaacgta
    59941 tttcggggcg attttgtgcg tctcgcaaca tctttccccg tcattttgtt actccgctta
    60001 catcaccctg attgatagta aaagtttgca acaagggtga aagtcagtac aatccctgcc
    60061 cgaatgtgtg taaacgtgaa cgcaatcgat tacgtaaatg atagaactgt gaaacgaaac
    60121 atatttttgt gagcaatgat ttttataata ggctcctctg tatacgaaat atttagaaac
    60181 gcaatttgcg cctttttcac tcccgcaagg gattttcaaa cagtggcata catatgaaaa
    60241 aaacattact ggcagccggt gcggtactgg cgctctcttc gtcttttact gtcaacgcag
    60301 ctgaaaacga caaaccgcag tatctttccg actggtggca ccagagcgtt aacgttgtcg
    60361 gaagctatca cacccgtttc ggaccgcaga tccgcaacga tacctacctt gagtacgaag
    60421 cattcgctaa aaaagactgg ttcgacttct atggttatgc ggatgcgccg gtattcttcg
    60481 gcggtaactc cgatgctaaa ggtatctgga accacggttc tccgctgttt atggaaatcg
    60541 aaccacgttt ctccatcgac aagctgacca atactgacct tagcttcggt ccgttcaaag
    60601 agtggtactt cgcgaacaac tacatttacg acatgggtcg taataaagat ggtcgccaga
    60661 gcacctggta catgggtctg ggtaccgaca tcgatactgg tctgccgatg agcctgtcca
    60721 tgaacgtcta tgcgaaatac cagtggcaga actatggcgc agcgaacgaa aacgagtggg
    60781 acggttaccg tttcaaaatt aaatactttg tgccgattac cgatctgtgg ggcggtcagc
    60841 tgagctacat cggcttcacc aacttcgact ggggttccga tttaggggat gacagcggta
    60901 acgcaatcaa cggtattaag acccgtacta ataactctat cgcttccagc catattctgg
    60961 ctctgaacta cgatcactgg cactactctg tcgtagctcg ttactggcac gacggtggtc
    61021 agtggaacga cgatgcagaa ctgaacttcg gcaacggcaa cttcaacgtt cgctctaccg
    61081 gctggggtgg ttacctggtt gtaggttaca acttctgatt atgaaaatgc cgggatttat
    61141 tcccggcatt tcagattgtt aacaacgtgc gctttgttca tgccggatgc ggcgtaaacg
    61201 ctttgtccga cccacaaaag cacgtaaatt caatatattg ctgaaataac gtagtcctga
    61261 taagcgtagc gcatcaggca attttacgtt ttaatcactt cttcttgttc atcatcgctt
    61321 tcaggtcagc gaatgggttg tacttcgctt caccgacatc tttctgcgca tcttcccctg
    61381 cgataacggt cgtaccatac tgatccgctt cggtatattt cgaatgctca tgatcgtggc
    61441 aatagagaca aagcaattcc cagttactgc catcttccgg gttattggta tggtcgtgat
    61501 caatgtggtg aacagtaagt tcacgcaggt tggaataaac aaactcgcgg gaacagcgac
    61561 cgcagaccca cggatatatt tttaatgctt tttcgcgata gccgctttct aaccgcgcat
    61621 agttttttgg gatgatagcc atgaagagtt acctggatga aatgtagggt tacgcgcagt
    61681 ttcccaagat gcggggattt tcgcaatctc catacatcaa aaaaaatccc gatcttctga
    61741 ccgggatttt caacatcaac gggaacttga ttacggcaga attgacggct gatccgcccc
    61801 ttctttttcc actttctgct gcaacatgtg ttcgcgcttc atacccagtt tcagagccaa
    61861 cgcagatgcc acatagatgg aagatgcagt accgatggaa acaccgataa gcatggtcag
    61921 cgagaagcct tccagtaccg gaccaccgaa gaggtacagc atcaggataa ccatcaaggt
    61981 agtaccggat gtgatcaagg tacggtgcag cgtctgggtc aaggacacgt taaagatttc
    62041 gtaaggcgta ccgcgacgga tcttgcggaa gttttcacga atacggtccg ataccacgat
    62101 actgtcgtta agcgagtaac cgataaccga catcaacgat gccacaatgg tcaggtcaat
    62161 ctcgatatgg aataacgaca aaatacccag cgtaatgata acgtcgtgcg ccagcgcaat
    62221 aaccacccct gccgccagtc gccactcaaa gcgaaaacct acgtacacaa ggatagacag
    62281 cagcgccgcc atcaacgcca tcgcaccggt ttgcgcaagg tctgccccca cgctcggacc
    62341 gacgaactca atacgcttca ctgctgcatt ctgattggtg gattcgttaa tcaccttcag
    62401 aacctggctg cccagcacct gaccgccggt ttcgccttca gcaggcggca tacggaccat
    62461 gatgtcgtgg ctgctaccaa agttttgcag cattggctct tcaaaaccgg ctttttgcaa
    62521 tgcatcacgc attacgtcaa tttcagccgg tttttcgagc gtaatttcga taaccgtacc
    62581 accggtgaaa tccagccccc agttaaagcc gcgcacgccc ataataacga tagcggcgat
    62641 taacagcaga ccagagatgc cgaaagccca gtagtcccag cgcataaagt catagacttt
    62701 acggccgtgg tttagttgtt caacagtata ttcctgtgcc acatcgcact cctcagattg
    62761 acagcttctt gacgcgcttg ccgccatata gcaggtttac gatggcacgc gtaccgacaa
    62821 tcgcggtaaa catcgacgtc gccacaccga taccggtagt aatcgcgaac cctttaattg
    62881 ccccggtacc cactgcgtac aggatgatga ctttaatcag cgtggtgatg ttcgcatcga
    62941 agatagaact gaatgcgcca cgataacctt catcaattgc ctgttgaaca gtacgtccgt
    63001 tgctcaactc ttctttaata cgttcgttga tcagtacgtt cgcatcgacc gccaccgcaa
    63061 gggttaagac gatacccgcg atacctggca tactcagcgt tgcgcctggc aacagcgaca
    63121 taatgccgac gattaagatc aagttggcaa tcagagcact ggtcgcaatc agaccaaact
    63181 tcttatagaa gatgatcatg aacagaatag aaaccagcag accggcaagg caagcctcca
    63241 gcccctgttc aatgttctgc atacccaggg ttgggccaat ggtacgttct tcaacaatct
    63301 gaatcggcgc gatcaacgca ccggcacgca gcagcagtga cagctgacgg gcttcgttcg
    63361 ggttgttgat gccggtgata cggaagctgt tacccagacg agactggatg ttggcgatgt
    63421 taatcacctc ttcctgtttc accagaaccg cacgaccatt tgcatctttc ttaccgctgt
    63481 ctttgtactc cacaaacagg gttgccatcg gtttgccgat gttgtcctta gtgaagttag
    63541 acatgatgtt accaccagcg ctatcgagcg agatgttaac ctgcggttgg ttgtattcgt
    63601 cctggctgga agtggagtcg gtgatatggt caccggtcag aattacgcgt ttgtacagca
    63661 caactggctg accttcgcgg gtctgtttca cttcagagtc gcccggtacg cgaccggatg
    63721 ccgcagcggc ctggtcaacg ttggtgttta ccagacggaa ttccagcgtt gccgtcgcac
    63781 ccagaatctc tttcgcacgc gcagtgtcct gaatacctgg cagttcaaca acgatacggt
    63841 cagcaccctg acgctgaacc accggctccg ccacgccaag ttggtttaca cggttacgca
    63901 ggatattgat gttctgctgc accgcatatt cacgcgcttc actcagacga gcatcgctca
    63961 ttaccgcacg cagctggttg ctgccctggc tactaatcac caggtccgga tggcgcttgc
    64021 tcagatacgc aatggcttca tcacgagctt tagcatcgcg gaaagtaatg ctcaggccgt
    64081 agttgttttc tttacgaacg gtggtatacg ggatgccctt ttcacgcaga tcgctgcgca
    64141 ggctatcgat attttgttcc tggagtttgc caagcgcggt gtccatatcc acttccatca
    64201 ggaagtgaac gccgccacgc aggtcaaggc cgagcttcat cggctcagcg tgaatagctg
    64261 ccagccagcg cggcgttgcc ggggcaaggt taagcgccac gacgtatttg tcacccataa
    64321 cgcccattaa tgcttcacga gcgcgcagct gggtgtcagt ggagtcgaag cgcgcaagaa
    64381 tagcgccctc ttccagtgcc acagacttag cagttatttt ttcttcttgt aacgttttct
    64441 ggacctggat cagcgtttgc tcactggcgg cgactccgcg cgcaccagtg atctgaacag
    64501 ccggatcctc accaaacagg ttgggaagcg catacagcag accgatgaca atcaccacga
    64561 tcagcatgac gtacttccac aaaggataac ggtttaacac ggcaattccc ttagggaaaa
    64621 attttaatta cagcgccttc atggtgcctt tcggcaggac ggcagctacg aagtcacgtt
    64681 taataactac ttcagtggtg tcattcagcg cgatagcaat gtagccgttt tccgctactt
    64741 tggttacgcg accaaccagg ccaccgttcg tcagaacttc atcacctttg gcaatggagt
    64801 ccatcagctt tttgtgttct ttggtgcgct tctgctgtgg acgcaggatc atgaaataga
    64861 aaatcagacc gaataccacc agcatcaaaa tcaaagacat cgggctacct tgcgccggtg
    64921 cacccgttgc cgctaccgca tcagaaataa aaaagctcat taaatttccc tcattattaa
    64981 tattaatcaa cgttcaaagg tggtacttct cgcccctgac gctggtaaaa atcagttacg
    65041 aagctctcta atttaccctc ttcaatagcc ttgcgtaaac ccgccatcaa acgctggtag
    65101 taacgaaggt tatgaatggt gttgagtcgc gcgcctaata tttcgttgca acggtcaaga
    65161 tgatgcaagt aagcgcgtga ataattgcga caggtgtagc aatcacactc aggatcgagt
    65221 gggccagtat cgctcttata cttcgcattg cggattttca ccacgccatc ggtcacgaac
    65281 aaatgaccat ttcgggcgtt gcgggttggc attacgcagt caaacatatc gataccacga
    65341 cgtacgcctt caaccaggtc ttctggttta ccaacgccca tcaggtaacg cggtttgtct
    65401 gccggaattt gcgggcatac atgctccaga atgcggtgca tatctgcttt cggctcaccc
    65461 acagccagac cgccgacagc gtagccatca aaaccgatat ctaccagacc tttaacagaa
    65521 atatcacgta aatcttcgta aacgctgccc tgaatgatac caaacagcgc atttttgttt
    65581 ccgagactgt caaaacgctc acggctacgc ttcgcccaac gcagagacat ctccatggag
    65641 cgttttgcgt aatcccagtc agcaggatac ggcgtacact catcaaagat catgacgata
    65701 tccgaaccaa gatcgtactg aatctccatt gatttttcag gatcgaggaa aatcggatcg
    65761 ccgttgatcg ggttacggaa gtgcacgccc tgttcggtga ttttacgaat atcgccaagg
    65821 ctgaagacct ggaagccgcc ggagtcggtg aggatcggcc ccttccactg cataaaatcg
    65881 tgcagatcgc cgtgcagttt catgatttcc tggcccgggc gcagccacag gtggaaggtg
    65941 ttgccgagga taatttgcgc gccagtggct tcaacttctt ccggcgtcat cccttttacg
    66001 gtgccgtagg tgccaacagg cataaaacaa ggcgtttcca ctacgccaca atcaaagacc
    66061 aggcggccac ggcgtgcgcg accgtcggtg gtgtccagtt caaatttcat tttttctcca
    66121 ctacgtcaga aaaacagtcc aacgttttaa accagcgccg cggaattact ccccgacgcg
    66181 ctcattaatt gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg
    66241 atatttctct tctaccgctg ctttataggc gttcatggtg tgttgataac cggcaaaggc
    66301 cgaaaccagc ataatcagcg tcgattccgg caggtggaag tttgtcacca gcgcatcgac
    66361 cactttgtac tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat
    66421 aagatcgttt ttcgccgcct gagccgcgct ttccagcgaa cgtactgagg tggtgccaac
    66481 cgcaatcacc cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg
    66541 cacttcagcg tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg
    66601 gaaggtgccc gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa
    66661 tttttccagc aaaggttcgt caaaatgcag acctgcggtc ggggctgcaa ccgcgcccgg
    66721 tttttcgcta taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacggtcgat
    66781 atacggcggc agcggcatat ggccgatgct gttgagaata tccagcaccg agcgttcatc
    66841 attaaattcg acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt taatactttc
    66901 gtcatcgccc agcagcagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc
    66961 aagaatgcgt ttgtcgtcga gcatccgttc aaccagcact tcaatcttgc cgccgctggc
    67021 tttacgccca aacaggcgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc
    67081 ggggttgagc ttatcaagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc
    67141 gtccagcgac agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga
    67201 ttcgggcaat tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag
    67261 aggcgggtag tctagtgccg gggcgctctc cctgcaacct ttaccccctt ttggatagaa
    67321 tataatcgat gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt
    67381 ccggcaattt actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg
    67441 tcgtggctgg cattcatatg catcgacgta tcgacgtatt aaccgacaat ctgccggaag
    67501 tccgcgaagc acgggaatgg tttcgtagtg aaactcgccg cgttgcgcct attacgctgg
    67561 atgtcatgtg ggatcacttt ctttcccgcc actggtcgca gctgtcgccg gactttccgc
    67621 tacaggaatt tgtctgttat gcccgcgagc aagtgatgac gattttgccg gactcaccgc
    67681 cacgttttat caatctgaac aattacttgt ggtcagagca gtggctggtg cgctatcgcg
    67741 atatggattt catccagaac gtgttaaacg gcatggcaag ccgccgccca cgtctggatg
    67801 ccctgcgtga ctcctggtac gatttagacg ctcattatga cgccctcgaa acccgcttct
    67861 ggcagtttta tccgcggatg atggcgcagg cgtcacacaa ggcgttataa aacgttagtt
    67921 catccatacc gtagccgaaa tggcaggcaa agccagaatc ccgtcagtca gttgcccatg
    67981 cccttctttg cattgccatt gcacggcatt gagcaacggt gacgcgggta gcaccacttc
    68041 acaagcttcg ccacggttga ttgccaccag tacacgctgc tgattcagca cgcggacaaa
    68101 taccaccacg ttatcttccg catacagcac ctgacagccg ccatgacgta gcgcctgact
    68161 tttcttacgc agcgcaatca ttcgctggta cagcgcgaat aacgccgtat cctgcttttc
    68221 cacctgccag gggaacggtt tacggcaaaa cggatcgttt ttgccatcca gtcctacttc
    68281 atcaccgtaa taaatgcacg gtacaccagg ccaggtgaac agccagacca ccgccagcgg
    68341 caggcgcgca atatcccgac cgagcagcgt tttaaatcgc gcagtatcgt ggctgtcgag
    68401 ctgattaaac atacgtaatt gttgttgatg agaaagccct gcgcggtaat tatccatcca
    68461 ggccatacag gtttgggcat caatttgctg cggatcgtaa gagatatcgg tattggcaag
    68521 aaatccccac aacgggaatg tgaagccacg atagttcatg gcggcatctt ccacatcggc
    68581 ctgtaaccat tgccgtgcat cgccaaaatg ttcgccgaca atatacgctt ccggctgggt
    68641 ttctttcgcc gcttcggtga tcccagcaac gtgctgcata ttattgcgcg ccccacccgc
    68701 ctcccccagc atatgcacca catccagccg ccagccgtcc atactccacg gcgctttcag
    68761 ccaatggcgg acaatactgt cttccccgcg ataaatttca ttcaccagac tttccgactg
    68821 ataatccagc ttcggcaagc tggcatagcc aagccagtcg agcgccgtgc catcatcact
    68881 aaacgagtac cagtcgcgcc agggcgattc agggttgtga caagctcccc ccgtgccacg
    68941 attgtgcctg tcaaaccagg catgggaatc gccactgtgg ttaaacacgc catccagcac
    69001 cagccgcatt cccagctgct gcgtattgtg gcgtaagcgc agcaacgccc catcaccgtc
    69061 aaactgcgga tcgacatggc gataatcctc ggtatcgtat ttatgtacgc tgggagcttt
    69121 aaacaccgga ttgagataca gcgccgtcac gccaagcttt ttcagatacg gcagtttttc
    69181 gcttatcccg tccagatcgc cgccatagaa cgttgatccg cccgcctgcg ccgtgaccgg
    69241 ttcatcccag tcacgcaaga tgatctcttg tccggctgca tgatggtaat agacatgatc
    69301 ctgttcagct tcacgaggaa gactacgcgc aaaacgatca gggaagatct gataaaaaat
    69361 ctgatccgca gcccattgtg ggccgatatc cggtacatcg acggcaaact gctccagtcg
    69421 tgccggcggc attcggctga agccctgcgg tgtaaaccaa cgctggcgat cgtgccacag
    69481 cagtttgaaa ctgtaacgcc gccggggttg tccgctggag agatcaatcg ccgcacgcca
    69541 tgcggtgacg ccaggctgct gctgactgcg ctgcttatgc atcgatactg acatttcttc
    69601 gttatcgtgt tctgtacgca gcataatgcg ctgcggtggg tcttcgcccg tcagccacag
    69661 tgtaatgagc agttgatctt tgctttgttt aacaaatggg ggcaccggca ggtgccatgc
    69721 atttaacatc ataattcccc tgtgatgaaa atctgatcac cttgccatag cctaatgcag
    69781 gatcactcat catctgaagg atatttgggg gaggaggacg ggggcggata actttcggaa
    69841 tagccggatc aggatttcct cctgaaccgg cttacagaac gcaagtatta ttggttttca
    69901 gccagctgac gatcgtggcg gcgtttaaac atccagccaa tcaacagcac aacaatccac
    69961 gcgaaaccga catacagcga gatacgcgta tccgggtgat aaccaatcaa cccgataata
    70021 aagagcagga aaatcagccc gccgatggtc gttgctaccc cacccggcac tttaaatttc
    70081 agcgccttaa cttcttctgg cggcaaacgg cgacggaagg caatttgcga cagcaggatc
    70141 ataatccaca cccacaccgt ggcgaaggtt gccagcgaag cgatcaccag gaagacgttt
    70201 tccggcatga tgtagttcag atacaccgca aacagcagcg cggtagtcat caccagcacc
    70261 gtaacccacg gaataccgcg acgcgacgtt ttgctgaaaa ttttcggcgc gctgccctgc
    70321 tctgccatac cgtggagcat acggcctacg ccaaatacgt cactgttaat tgccgacagc
    70381 gaagcagtca gcacaacaaa gttaagaatg ctggcggcaa aggtaatgcc catatgctgg
    70441 aacgtcagca cgaacgggct accggcagtg ccaacctgat tccacgggta gatagacata
    70501 atgacgaaca gcgtaccgac gtagaacacc agaatacgca tcggcacgga gttaatcgca
    70561 cgcggtatcg atttctcagg atctttcgct tcaccggcgg taatcccgat aatttcgatc
    70621 ccaccgtaag caaacatcac catttgcaac gacatcacca tcccaagcca gccgttactg
    70681 aagaagccgc cgttgctcca caggttatga ataccggtcg gttgcccgcc gttgccaatc
    70741 ccccagatga tgatgccgaa accggcgaca atcatgatga tgatggtggc gactttaaag
    70801 aacgagaacc agaattccag ctcaccgaag accttcacgc tcatcaggtt tacggcgcaa
    70861 atgatcagca ccacgctcag tacccaaatc cagtgcggca ccgtcgggaa ccagacaccc
    70921 atatagatac caaaagcggt cacatcggcg atggcgacaa taaggatttc aaagcaatag
    70981 gtccagccgg taatgtaacc tgccagcggg ccgaggtttt cctgcgcata acgcgagaaa
    71041 gagctggctg ccgggttatg taccgacatt tcccccagcg cacgcataat gatatacgcc
    71101 gcgataccac cgataatata ggccaacaac acgctcggac cggccatttt gatggcgtct
    71161 gccgaaccgt aaaacagccc ggtgccaatt gctgaaccca gtgccataaa gcgtatgtgg
    71221 cgggtactta gcccacgctt tagcttgttc ttactttcca tcaattccta acccatcaac
    71281 acaataaaaa accacggggc atcgcagccc cgtggttaaa acaaatgttc agtgatttag
    71341 tgagcgctgg aggtcacctg acgacctgcc gcgcgatccc agataatggc cagaaccacc
    71401 atcaccactg ttggcattaa ccacgccaga ccttgttcgg ccagcggtaa acgctgcgcc
    71461 caggacggta agatatcgct gaatgcagat gctttgatcc cgtcgagaat accaaaaagc
    71521 aggctgataa acatcggcgg agcaatcacg cgggacgaat tatgccacca tgagcgtgta
    71581 aaacttaata caaccagtgc gatacacggc ggataaatag cggtcagcac cggtacggag
    71641 atctggatca gctggcttaa gccgaggtta gaaaccacca tcgagaagcc gccgaggata
    71701 aacaccagcg tacgataaga gagcggtacg tactgggcaa agaattctgc acaagcacag
    71761 gtcaggccaa ctgccgttac caggcaggcg atgaagatta acgccgccag caggaagcta
    71821 ccgccgccgc caaaggtgtg ctgaacgtaa gcatgcagaa tagcagcgcc gtttgcagac
    71881 tgatcgacca gcgacgcgct gtctgaaccc agacggaaca gcgccaggta cagcagagtc
    71941 agaccaacac ccgccatcag gccagcccag acggtataac gggtcagcag acgcgcttcg
    72001 gtaacgccac gagaacgcgc cgcgttaaca ataacgatac caaacaccat tgcgcccagc
    72061 gtatccatgg tcagatagcc gttaacgaag ccgttagaaa acgcagcgtt ttgataagcc
    72121 tcagtcgccg tgctgataga acccgccggc cagacaatcg cggcaacaga caggatgacc
    72181 agcgcgataa ttttcagcgg cgcaaggaag ttgcccacgg tatccagcag cttgcccgga
    72241 tagagcgaaa ccagaataac gatagcgaaa tagaccaggc tgtagataaa cagcggcagc
    72301 gcggaatcac ccgtcagcgg cgcaatcccc acttcaaagg aaacggtagc tgtacgcggc
    72361 gtagcgaaaa gcggccccac cgccaggtaa caaaccgttg ccagcagtac gccagcgact
    72421 ttaccgattg gcgtgctgag gctgtcaaca ccgccgccaa cttttgccag cgccactacc
    72481 gttaataccg gcaggccaac ggcagtaatg aggaagccga atgccgcagt ccagacgtgt
    72541 tcgcctgcct gtaagccgac cattggaggg aaaataatgt tacctgcgcc gacgaacaac
    72601 gcaaatgtca taaagcccag agcgatgata tcgcgcgatc ttaattgatg ggtcataaat
    72661 catactgcct gtggatgtgg tgttgtaacg ttaaattttt gtgccaatcc ctggggatga
    72721 tacagcggta tatttaaaca attttagcgg gaaatactcc caatcaggcg cggcgaagaa
    72781 tagtttttcg atttaccgcg ttaatgcagt cagtatgaca gcacctgaag cgcaatttaa
    72841 acgcttataa cgttttaaag caaggaggga tctaaaaacc agaggtatat agcgataaga
    72901 aatcaccaac cagtataaaa cactatttcc gtgaaaaaga catggctaat catgcgaaca
    72961 aaaaatcacc aaccgttgta aaatctaacg cccgtatttt gactgcaaag aaaataagcc
    73021 agcatcggct ggcttatggc aataaaagat gacaaaggcg gattaatcgc tgtttttggc
    73081 aattaaacgt tccgggataa caaaactgaa acgtgttcct tttcctactg tactctcaat
    73141 attcaggcga ctttcgtgat gattcaccgc atgtttcacg atcgctaacc ctaatccgct
    73201 accgccggtt tgccgggaac gcgctttatc aacgcgataa aaacgctcgg tcaggcgcgg
    73261 aatatgctcc ggtgcaatgc ccggtccgtt atcttcaacg ctaaattcgg caccgtgcgg
    73321 cactcgcagc cagcgtacgg tgatatgcgt gccttccggc gtatgattca cggcgttata
    73381 gaccaggttc gaaatcgcac tgcgtagctg atcttcgttg ccagacacct tgaggccgtt
    73441 atctatctca aaggtaaatg tctgtttttt ctgactcaga gtctgagcct cgcgctcaac
    73501 aacgcgcagc atcatcggca catcaacctt ttcattgagc aattgcgtcg gcgcggcttc
    73561 aattttcgac agcgttagca actgcttcac cagcccttcc atccgctggg tctgctcgcg
    73621 catggtgtgc aacgcttttt cgcgtaccgc gccttccagc ggctgctcat ccatcatctc
    73681 caggtaaccc tgtaacacgg tcaatggcgt acgtaactca tggctcacgt tggcaaaaaa
    73741 gttacgccgc gccccttcca gttgatgcat ttgcgtgaca tcacgcgcca ccatcagcaa
    73801 ctgtttgtgg gtataaggca tgacgcgaat ttccagatgc cgcccggtgt tgagcaccag
    73861 attgagcggg cgagaaaaat cacgcgtttt cagatattgc gtaaactccg ggtaacgcag
    73921 caggttaagg atgttctgcc cgttatcttc cggccagcgc aaaccaagaa tttgttgcgc
    73981 cagaccgtta caccagaaaa taccgccctc ttccgtggtc agcaccactg cgtcgggcag
    74041 cgactccgcg ccgctacgaa agcgtttaat cagattgccc agttcacggc ggcgtttttt
    74101 atttcgcagc tgcatctggt gtaagccgta tagtagcggt tcccagctac cacgccccgg
    74161 tggcggggtc atactacgat ccacccacag ccaccatgaa aggcgcaata aattccagaa
    74221 atgccagata agcagtcccg ttaccgatgc cagcaaaaac cagggcaggt aaccaaaaaa
    74281 tgcacccagg atgaaagccg ggaggcagca aagtagcagc tccagcacca gccttttcca
    74341 cgacagccgt tccagcacgt aagatactcc agttaagaaa tcataagccc tgctctgcgt
    74401 ccgatgagca aggcgttaaa agcgggttga aaaacgatat cctgtaccgc gcacggtctg
    74461 caccatgcgg tcatgcccgc cgggctccag tgctttacgc aggcgacgaa tgtggacatc
    74521 gaccgtgcgg tcttccacat acacgttagt tccccagacg tggtttaaca gctgctcgcg
    74581 gctgtacacg cgctcaggat gcgtcataaa aaagtgcagc agtttaaatt ctgtcggccc
    74641 catctccagc ggctcttcgc ccgccatcac tcggtgagat gtcgggtcga gacttaatcc
    74701 ctgcatctca atcacctctt ccaccgccat tggcgaaata cggcgcatta ccgctttgat
    74761 tcgcgccacc agctccttcg gcgaaaacgg cttggtgata tagtcatccg cgccggtttc
    74821 aaggccgcgc acgcgatctt cttcttcccc tctggcggtc aacatcacca ctggaatatc
    74881 ccgggtcatc gactcgcgct tgaggtgttt gatgaactgg ataccggagc cgccaggtaa
    74941 catccagtca aggagaatta aatccggcca gggttcattc agttgattca cagcactgtc
    75001 ataatcttcc gcttcgaccg gctgaaagcc attttgttcg agcacgaagc agaccatttc
    75061 gcgaattgga gcttcatctt ctacgaccag aatacgtctc gccatgattt gccctgttgt
    75121 aataaatagg ttgcgatcat taatgcgacg tcattatgcg tcagatttat gacagattta
    75181 tgaaaagctc gtcgcacata tcttcaggtt attgatttcc gtggcgcaga aaaaagcaaa
    75241 tggcacatct gtttgggtat aatcgcgccc atgctttttc gccagggaac cgttatgcgc
    75301 atccttcaca cctcagactg gcatctcggc cagaacttct acagtaaaag ccgcgaagct
    75361 gaacatcagg cttttcttga ctggctgctg gagacagcac aaacccatca ggtggatgcg
    75421 attattgttg ccggtgatgt tttcgatacc ggctcgccgc ccagttacgc ccgcacgtta
    75481 tacaaccgtt ttgttgtcaa tttacagcaa actggctgtc atctggtggt actggcagga
    75541 aaccatgact cggtcgccac gctgaatgaa tcgcgcgata tcatggcgtt cctcaatact
    75601 accgtggtcg ccagcgccgg acatgcgccg caaatcttgc ctcgtcgcga cgggacgcca
    75661 ggcgcagtgc tgtgccccat tccgttttta cgtccacgtg acattattac cagccaggcg
    75721 gggcttaacg gtattgaaaa acagcagcat ttactggcag cgattaccga ttattaccaa
    75781 caacactatg ccgatgcctg caaactgcgc ggcgatcagc ctctgcccat catcgccacg
    75841 ggacatttaa cgaccgtggg ggccagtaaa agtgacgccg tgcgtgacat ttatattggc
    75901 acgctggacg cgtttccggc acaaaacttt ccaccagccg actacatcgc gctcgggcat
    75961 attcaccgcg cacagattat tggcggcatg gaacatgttc gctattgcgg ctcccccatt
    76021 ccactgagtt ttgatgaatg cggtaagagt aaatatgtcc atctggtgac attttcaaac
    76081 ggcaaattag agagcgtgga aaacctgaac gtaccggtaa cgcaacccat ggcagtgctg
    76141 aaaggcgatc tggcgtcgat taccgcacag ctggaacagt ggcgcgatgt atcgcaggag
    76201 ccacctgtct ggctggatat cgaaatcact actgatgagt atctgcatga tattcagcgc
    76261 aaaatccagg cattaaccga atcattgcct gtcgaagtat tgctggtacg tcggagtcgt
    76321 gaacagcgcg agcgtgtgtt agccagccaa cagcgtgaaa ccctcagcga actcagcgtc
    76381 gaagaggtgt tcaatcgccg tctggcactg gaagaactgg atgaatcgca gcagcaacgt
    76441 ctgcagcatc ttttcaccac gacgttgcat accctcgccg gagaacacga agcatgaaaa
    76501 ttctcagcct gcgcctgaaa aacctgaact cattaaaagg cgaatggaag attgatttca
    76561 cccgcgagcc gttcgccagc aacgggctgt ttgctattac cggcccaaca ggtgcgggga
    76621 aaaccaccct gctggacgcc atttgtctgg cgctgtatca tgaaactccg cgtctctcta
    76681 acgtttcaca atcgcaaaat gatctcatga cccgcgatac cgccgaatgt ctggcggagg
    76741 tggagtttga agtgaaaggt gaagcgtacc gtgcattctg gagccagaat cgggcgcgta
    76801 accaacccga cggtaatttg caggtgccac gcgtagagct ggcgcgctgc gccgacggca
    76861 aaattctcgc cgacaaagtg aaagataagc tggaactgac agcgacgtta accgggctgg
    76921 attacgggcg cttcacccgt tcgatgctgc tttcgcaggg gcaatttgct gccttcctga
    76981 atgccaaacc caaagaacgc gcggaattgc tcgaggagtt aaccggcact gaaatctacg
    77041 ggcaaatctc ggcgatggtt tttgagcagc acaaatcggc ccgcacagag ctggagaagc
    77101 tgcaagcgca ggccagcggc gtcgcgttgc tcacgccgga acaagtgcaa tcgctgacag
    77161 cgagtttgca ggtacttact gacgaagaaa aacagttact taccgcgcag cagcaagaac
    77221 aacaatcgct aaactggtta acgcgtcagg acgaattgca gcaagaagcc agccgccgtc
    77281 agcaggcctt gcaacaggtg ttagccgaag aagaaaaagc gcaacctcaa ctggcggcgc
    77341 ttagtctggc acaaccggca cgaaatcttc gtccacactg ggaacgcatc gcagaacaca
    77401 gcgcggcgct ggcgcatatt cgccagcgga ttgaagaagt aaatactcgc ttacagagca
    77461 caatggcgct tcgcgcgagc attcgccacc acgcggcgaa gcagtcagca gaattacagc
    77521 agcagcaaca aagcctgaat acctggttac aggaacacga ccgcttccgt cagtggaaca
    77581 acgaactggc gggttggcgt gcgcagttct cccaacaaac cagcgatcgc gagcatctgc
    77641 ggcaatggca gcaacagtta acccatgctg agcaaaaact taatgcgctt gcggcgatca
    77701 cgttgacgtt aaccgccgat gaagttgcta ccgccctggc gcaacatgct gagcaacgcc
    77761 cactgcgtca gcgcctggtc gcgctgcatg ggcagattgt tccccaacaa aaacgtctgg
    77821 cgcagttaat ggtcactatc cagaatgtca ctctggaaca gacgcaacgt aatgtcgcac
    77881 ttaacgaaat gcgccagcgt tataaagaaa agacgcagca acttgccgat gtgaaaacca
    77941 tttgcgagca ggaagcgcgc atcaaaacgc tggaagccca gcgtgcacag ttacaggcgg
    78001 gtcagccttg tccactttgt ggttccacca gccatccggc ggtcgaggcg tatcaggcgc
    78061 tggagcctgg cgttaatcag tctcgattac tggcgctgga aaacgaagtt aaaaagctcg
    78121 gcgaagaagg tgcggcgctg cgcgggcaac tggatgcatt aacgaagcag cttcagcgcg
    78181 atgaaaacga agcgcaaagc ctccgacaag atgagcaagc acttactcaa caatggcaag
    78241 ccgtcacggc cagcctcaat atcaccttgc agccacagga cgatattcaa ccgtggcttg
    78301 atgcacaaga tgagcacgaa cgccagctgc ggttactcag ccaacgacat gaattacaag
    78361 ggcagattgc cgcgcataat cagcaaatta tccagtatca acagcaaatt gaacaacgcc
    78421 agcagcagct cttaacggca ttggcgggtt atgcactgac attgccacag gaagatgaag
    78481 aagagagctg gttggcgaca cgtcagcaag aagcgcagag ctggcagcaa cgccagaacg
    78541 aattaaccgc gctgcaaaac cgtattcagc agctgacgcc gattctggaa acgttgccgc
    78601 aaagtgatga tctcccgcac agcgaagaaa ctgtggcgct ggataactgg cggcaggttc
    78661 atgaacaatg tctcgcatta cacagccagc agcagacatt acagcaacag gatgttctgg
    78721 cggcgcaaag tctgcaaaaa gcccaggcgc agtttgacac cgcgctacag gccagcgtct
    78781 ttgacgatca gcaggcgttc cttgcggcgc taatggatga acaaacacta acgcagctgg
    78841 aacagctcaa gcagaatctg gaaaaccagc gccgtcaggc gcaaacgctg gtcactcaga
    78901 cagcagaaac gctggcacag catcaacaac accgacctga cgggttggct ctcactgtga
    78961 cggtggagca gattcagcaa gagttagcgc aaactcacca aaagttgcgt gaaaacacga
    79021 cgagtcaagg cgagattcgc cagcagctga agcaggatgc agataaccgt cagcaacaac
    79081 aaaccttaat gcagcaaatt gctcaaatga cgcagcaggt tgaggactgg ggatatctga
    79141 attcgctaat aggttccaaa gagggcgata aattccgcaa gtttgcccag gggctgacgc
    79201 tggataattt agtccatctc gctaatcagc aacttacccg gctgcacggg cgctatctgt
    79261 tacagcgcaa agccagcgag acgctggaag tcgaggttgt tgatacctgg caggcagatg
    79321 cggtacgcga tacccgtacc ctttccggcg gcgaaagttt cctcgttagt ctggcgctgg
    79381 cgctggcgct ttcggatctg gtcagccata aaacacgtat tgactcgctg ttccttgatg
    79441 aaggttttgg cacgctggat agcgaaacgc tggataccgc ccttgatgcg ctggatgccc
    79501 tgaacgccag tggcaaaacc atcggtgtga ttagccacgt agaagcgatg aaagagcgta
    79561 ttccggtgca gatcaaagtg aaaaagatca acggcctagg ctacagcaaa ctggaaagta
    79621 cgtttgcagt gaaataacta ttcagcagga taatgaatac agaggggcga attatctctt
    79681 ggccttgctg gtcgttatcc tgcaagctat cactttattg gctacggtga ttggtagccg
    79741 ttctggtagt tgtgatggtg gtatgaaaaa agtcatttta tctttggctc tgggcacgtt
    79801 tggtttgggg atggccgaat ttggcattat gggcgtgctc acggagctgg cgcataacgt
    79861 aggaatttcg attcctgctg ccgggcatat gatctcgtat tatgcgctgg gagtggtggt
    79921 cggtgcgcca atcatcgcac tcttttccag ccgctactca ctcaaacata tcttgttgtt
    79981 tctggtggcg ttgtgcgtca ttggcaacgc catgttcacg ctctcttcgt cttacctgat
    80041 gcttgccatt ggtcgcctgg tttccggctt tccgcatggc gcattttttg gcgtcggcgc
    80101 gatcgtgtta tcaaaaatta tcaaacccgg aaaagtcacc gccgccgtgg cggggatggt
    80161 ttccgggatg acagtcgcca atttgctggg cattccgctg ggaacgtatt taagtcagga
    80221 atttagctgg cgttacacct ttttattgat cgctgttttt aatattgcgg tgatggcatc
    80281 ggtctatttt tgggtgccgg atattcgcga cgaggcgaaa ggaaagctgc gcgaacaatt
    80341 tcacttttta cgcagcccgg ccccgtggtt aattttcgcc gccacgatgt ttggcaacgc
    80401 aggtgtgttt gcctggttca gctacgtaaa gccatacatg atgtttattt ccggtttttc
    80461 ggaaacggcg atgaccttta ttatgatgtt agttgggcta gggatggtgt tgggaaatat
    80521 gctaagtggc aggatttcag gacgttattc accactgcgc attgcagcag tgactgactt
    80581 tataattgta ctggcactgc tgatgctctt tttctgctgc ggcatgaaaa caacgtcgct
    80641 tatttttgct tttatttgtt gcgcgggatt atttgccctt tcagcacctc tgcaaatatt
    80701 gttactacaa aacgccaaag gcggagagtt attaggtgcc gcaggtgggc aaatagcgtt
    80761 taacctcggt agcgccgtcg gcgcatattg cggaggtatg atgctgacgc tggggctggc
    80821 atataattac gtggcgctgc ctgccgccct gctttcgttt gctgcgatgt catcgttgct
    80881 gctgtatggt cgctataagc gccagcaagc ggcggatagt ccggtgctgg cgaaaccact
    80941 ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg atgcggcgtg aacgccttat
    81001 ccgccctacg cggttctggc acattttgta ggcctgataa gacgcggcaa gcgtcgcatc
    81061 aggcatgatg cgccaattgc ctacgttttt tactcttgtg gccataacca cgcagcgccg
    81121 cgtacgccgc tggaatcacc gtgcttcgcc ttacgcaccg gcgtttcaca ttcgccgccg
    81181 aagacaaatt gtttaatcaa ctgcggaacc gtttgatata aacggtctac attgctcatc
    81241 ccgcccccca ggacaatcac atccggatcg agaatattca cgacatgtgc cagcgatttt
    81301 gccagccgca gctcgtagcg acgcaatgcc agttccgcta ccggatcgct ttcttcaacc
    81361 aggctgataa tttcactgcc tttcagcgca tgtccgctca aacgacgata atccgtcgcg
    81421 aatccagtgc ccgaaataaa ggtttcaata caaccttgtt taccgcaata acaagggact
    81481 tcctcgcgat aacgcagttc gtcttcgtcc atccacggta gcggattgtg tccccactca
    81541 cctgccgtgc cattgccgcc gatatgcgcc cgcccattga atgccacgcc cgcgccgcat
    81601 cccgtgccga taatcacggc aaataccgtc tgcgctcccg ctgccgcgcc atctaccgct
    81661 tctgaaaccg ccagacagtt ggcgtcattt gccagccgca cttcccgctg caaccgcgcg
    81721 cttaagtctt tatcgaatgg ctgaccgttg agccaggttg aattggcatt cttcaccaca
    81781 ccggtgtagg gcgaaatcga acctggaatg cccataccta ccgttccgcg ctgccctgtc
    81841 gcctgctccg ccatatcaac caacgtggcg atcgtttcaa tagtttgccg gtaatcatca
    81901 cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg catcgcccag tgcaatcact
    81961 tcagttttgg tgccgcctaa atcgatacct atacgcacgg tactctcctt atttttttca
    82021 atatcaatag cgtagagacg gacaaccgga ttggcaatgc aaggccgccg acaattcgtt
    82081 atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat
    82141 ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag
    82201 ctagcctcga tggcatttac cccatgcggc agccaggaca tggcgaagat gggctgggtt
    82261 cctccgatgg gatcgcacag cgatgcgtta acgcacgttg ccaatggtca aattgttatc
    82321 tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga ttaaacaggc gctggaagcg
    82381 aaaatcgcca aactggaagc ggaacaggcg cgtaagctga agaaaaccga aaaagattcg
    82441 ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt tcagccgttt tagccagaca
    82501 atgatgtgga tcgacacggt taacggtttg attatggtgg actgcgccag tgccaaaaaa
    82561 gcggaagata cgctggcatt actgcgtaaa agcctggggt cgttaccggt tgtaccattg
    82621 agcatggaaa acccgattga actgacgctg accgaatggg ttcgctccgg tagtgcggca
    82681 cagggcttcc agctgcttga tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg
    82741 atccgcgcga agaaacaaga tctgaccagc gaagagatca ccaatcacat tgaagccgga
    82801 aaagtggtga ctaaactggc gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac
    82861 gatggttcgc tcaagcgtct gaagttctgc gacgagctgc gcgatcaaaa cgaagatatc
    82921 gaccgtgaag atttcgccca gcgttttgat gccgatttca tcctgatgac tggtgaactg
    82981 gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg aagcacaacg ttaattgctg
    83041 attttccttt aatgccggat gcgacgcctg ccgcgtctta tccggcgtac gaagccacac
    83101 caggcatata attattcgct acggcgggca ataattttta gcgcagcaat attatgcgtt
    83161 ttatgctgta acttgctcca tggacgttgt gtcattgttt ttcctcaagc cagtgacgcg
    83221 cttcactgcg aattttccgc attgccccgg ttttcactaa atcccgtaac agcgaaagat
    83281 cgggttgttc gacaaataag agtaatgatg gatcctcttt taaccacgtc gttttcacat
    83341 cggcagcgag ctgtggatta ttcatagcta gcgaccgatg ccgagactcg gtcaatgtcg
    83401 ttaacaacga cgccggggta tgtgcgttgc gtaatgccgt ttggcgcaaa tgatcattgc
    83461 gactttgcag caatgcacgc caccattcat catctgattt atcttttatc gtagccagtt
    83521 ccgcaacaat atctggagat cttcactgat atttatatca accttgatga attatgaata
    83581 taccggaaat tgaaaaaatc gcagcaaagg aaatattatt atgggttaaa gagaggatat
    83641 ttcagaaaaa tgccctcccg ttcaagggga aggcgaggaa ttacagatag cggcacagat
    83701 aagaggtggg ttcggcaact tgcagatgaa actcactgtg accgggaaca ttaaacaccg
    83761 aaccggcttc atacacctgc cagtcggtcg cgtcaggcag taacacattc agcgcgccac
    83821 tgattaccgt catctcttcc ggctcagcgg tgctgaaggt gtattcgcct tcaaccataa
    83881 cacccacgct ggcgcgacca gtgctgctgc tggaaaagcc gattgatttc actttgccgg
    83941 aaaagtactc attactttga agcataaact ggccctgtct ggtagttgaa catctacttc
    84001 actatagggg ccagaggcgc tggctgtcac gcaaaattac accagtaatt ccgcagccag
    84061 ccgtgcaatc aatacgttag acagcaagac aggaacatcg agctgttttt gcagtaaatc
    84121 gcgatgacgt tggtgaaatc ctaaacaatc cagcatgatg acatcagccc cttttgccag
    84181 taattctttc ccggcatcaa tgattttttg ttctgaatca tgaatggggt tgcccaatga
    84241 aaataccggc gatttctgca aaatttgcca tttttgcgcc tgaacggtca gcatctcctc
    84301 aaccggaacg ataaccccca cctgatgatc ttcaacaata gaggaaacca gtggaggcaa
    84361 tattcgcgac ggctcaagaa agatcgtatt acgcgcagtc atactactaa tgtttgctgt
    84421 actcattaat aaaatgacgt cataaccctg attatcgagc acttcaacca caccttgcag
    84481 gtctcgctcc actttacgac gcgaaacatg ggccagctgg ttgtcattta ataatgtgag
    84541 aatggtgtct tcgcctgctt ctggcgcgta ctccgccatc acttcttcac gacttaactt
    84601 ccccagcaag ctatgatggg aaatattatc ttcgtcaatg tattccgtca ggagcggcaa
    84661 aacctcctgc ataggtacaa tgccgatggt caggatcgcc aacgacgcac tcattgttca
    84721 tccaccttac ttttctttcg tcgttaatta ccgggcaagt gtgaagcacc actgctgaca
    84781 cttacttgtg ctacaaatga caaaaagcgt agcagcagac gtgcacggca taatgtaaag
    84841 attccaaatg attccagtaa tggattgtta ttgtttaata ttctaattat tagaaaaaca
    84901 tgaattatga aaaatgtgac gcagatccct tgtagtggca ctcagaatcc cttccgagca
    84961 gtctgatgcg gtcactcttt gtcagggtcc tcatagcgtt ttttcgcatc catcgcttcc
    85021 tgagccgtcg gatgttcact gatcaacgag tctggtttag gatgatcggc tctgagttgg
    85081 taccaggtta ccgtctgtcc tggctttcct ttttcaatcg tcactatata tgcttcacga
    85141 ggataaggcg gtttcgttgg catcgttctt ccttatttca cgggatgaac gttaagtata
    85201 ggcgctctaa aatcaacaat tgatcgtctg tgccagggcg ctacgaattt cagaaatcac
    85261 ctggctgggt tcgtttgttg cgtcgatgat aatatgcgca acttcgcgat atagcgcatc
    85321 gcgttcttcc agcacttcct gaacttcttc gctcagcggt tttcccgtta aggttggccg
    85381 taaatcttct tccggtgcag cttgcagtcg gttaaccagg actgatactg gcgcacacaa
    85441 ataaaccacg atcccgttat tttgcatgaa gtgacgatta aattccgtca gaataatgcc
    85501 gccgcctgta gcgataacgg tggatggcgc agttaccgct tccagcgccg ccgtttctct
    85561 ggcgcgaaat cccgcccact cttccctttc gacgatctcc gcgaccgtca tattgagctg
    85621 tgattgcaac cactgatcgg tatcgacaaa ccgacggtta agcgaatcgg caagggccat
    85681 tccgaccgtt gttttaccac agccccgagg cccgatcaga aaaagaggtt gtgtcatcgt
    85741 gggttttccc caataggtcg caatgcggcg aaagccggtg tcatgagaat agcgatcatg
    85801 ccatcaaact agtacaattt cgattgtaaa gaaaaaattc cacttaaagt gaaaatctca
    85861 atacatccct tactatacca ataaatattc aagaatgaag tgtaaataat aaattacatt
    85921 tagccacgac tacgttgcac ttccagccac cacttctcca gctccgcggc aaaggcctgg
    85981 cggtcacgtt gtgaaaggct atctggtccg ccggtctgga tcccactggc acgtaaggta
    86041 tccataaaat cgcgcatcgt caggcgctca cgaatggtcg ctggcgtgta acgttcgccg
    86101 cgcggattaa gcgccgctgc gcctttctcg atggcttcag cagccaaagg tatatctgcg
    86161 gtgatcacca aatcgcccgc ttcacactgc cggacaattt cgttatcggc aacgtcgaaa
    86221 cctgccgcga cgcgcagcgt acgaataaat cgcgatggcg gcacgcgtaa actctggttt
    86281 gctaccagta ccagcggcat ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt
    86341 acattgggac acgcgtcggc atccacccaa attgtcataa agtcatcctt tgttgggtaa
    86401 tcctctattg tgtcgcgctt ttgccttccg gcatagttct gtttatgctt ctgccagcga
    86461 ttatcaaaac aatgaatttc acggcaggag tgaggcaatg gaaaagaaaa tcggttttat
    86521 tggctgcggc aatatgggaa aagccattct cggcggtctg attgccagcg gtcaggtgct
    86581 tccagggcaa atctgggtat acaccccctc cccggataaa gtcgccgccc tgcatgacca
    86641 gttcggcatc aacgccgcag aatcggcgca agaagtggcg caaatcgccg acatcatttt
    86701 tgctgccgtt aaacctggca tcatgattaa agtgcttagc gaaatcacct ccagcctgaa
    86761 taaagactct ctggtcgttt ctattgctgc aggtgtcacg ctcgaccagc ttgcccgcgc
    86821 gctgggccat gaccggaaaa ttatccgcgc catgccgaac actcccgcgc tggttaatgc
    86881 cgggatgacc tccgtaacgc caaacgcgct ggtaacccca gaagataccg ctgatgtgct
    86941 gaatattttc cgctgctttg gcgaagcgga agtaattgct gagccgatga tccacccggt
    87001 ggtcggtgtg agcggttctt cgccagccta cgtatttatg tttatcgaag cgatggccga
    87061 cgccgccgtg ctgggcggga tgccacgcgc ccaggcgtat aaatttgccg ctcaggcggt
    87121 aatgggttcc gcaaaaatgg tgctggaaac gggagaacat ccgggggcac tgaaagatat
    87181 ggtctgctca ccgggaggca ccaccattga agcggtacgc gtactggaag agaaaggctt
    87241 ccgtgccgca gtgatcgaag cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa
    87301 atcctgatga ctttcgccgg acgtcaggcc gccacttcgg tgcggttacg tccggctttc
    87361 tttgctttgt aaagcgccaa atctgccgat ttcaaccact cacgatagtg actcatttgt
    87421 gggttcagcg gcgcaacccc cacactaatc cgtaaagtta cctgtggcgt attcggcaag
    87481 cgtaacgtgt ttagcccttc acgcacccgt aacatggcgg taatggcgct ctcagctggc
    87541 gtaccggaca tgattactgc aaactcatcg ccgccaaacc gaccaatcac atcgctaccg
    87601 cgcagggtaa tttgtaactg tcgggtaagc gccacaatcg cttcatcgcc cacatcatga
    87661 ccccaggtat cgttgatgct cttgaaatgg tcgatatcga taatcagtaa cgttgcatcg
    87721 cgattatgcc gccgacagtt atcaaattca ttgcgtaaca tagtttccca atgacgtcgg
    87781 ttatacacgc ccgtcatgcc gtcgcgggta ctcatgacct gcaacctgcg tttatgttcc
    87841 gccagtttgg ttgccgtctg gtagctgacc cagccaaaca gcagaggata aacgacaata
    87901 atgggaaggg agagccacca ttccagcggc gcactattga acgacacggt aatgcccgta
    87961 agctcgaggg tgacaaggca ggaaaccacc atcaacacca gacccgcgac aaacagacgg
    88021 gggccgcctg cccccatcaa attcagacac ataatcatca acatagcggt ggaaggcagc
    88081 acgtttacgc ccattacgcc tacccacatt cccgctaata ctgcatcggt ttttaagttg
    88141 taaatttccc ggctaagcgg atcgacggcc ctgctcgcta tctgccaggc taaatgcggc
    88201 cagacgaacg cccagccgac caacaccagc caccaccagc ccggcggcgg gtgcgaaacc
    88261 agcgttgaag caatcggtaa gaacatgcca gccaggccaa ccgcacgggg tagtctgacg
    88321 cgacgggcga agcgcagccc ggaccgctga tgttcgtttt gaggagttaa aggaggttcc
    88381 tccccatgcg ccgccgcttt tttgaaaaag ttttcatcat tcattatttt tgggaacatt
    88441 cagaaacaat tttcccaaat tatagagacg gggctaaagg cagaagtatg aaatttcggg
    88501 tgagccactt tcgtagctca cccgttagca ctatcaggta tcaggcggct ttcttgaggc
    88561 aggcactcat aaacttatta cgatcatcac ctttcagaga ttgttgcgtt gcttgattat
    88621 tacattcgcg catcttttgc tgctgtggcg tcaaactttt ttcaccaggc gcagacttgc
    88681 tgttcttcag gcaatcactc atgtaggtct tacgagcatc ccctttcaac gcctgcgccg
    88741 tcgcctgctg attacaggat gtcatacgct gttgttgtgg ggttaaggtt ctctcggcag
    88801 cgccgacggt ggttaaaaaa accagaccaa aaagcaaggt aaccagtaat gttattttca
    88861 tagcaccatc cctcttcatg ttttaaccat gagcgtatgc gcccgtgatc tgccattaag
    88921 tctggttgct aacagcaaaa aaaccacccg gcagcgaaaa ttcactgccg ggcgcggttt
    88981 tatttcagcc ccagggcggc tttcatggtg tagaagagat cggtctggtc ggtcagtcca
    89041 acaacattgg cggcatgcgg gccatacgcc gcaatacgca actgactgcc ggtatgttct
    89101 tgtgaatcct cttcggagtt cccgtagctc atcaccatca ctgcgccatc tttggtattt
    89161 agcgcctggg tgaggcccgg agctttggta tccggcgcga caatctggct ggcgtgggcg
    89221 tgatcagcgg tgactatgac cagcgtgttg ccatcctttt tagcgaattc cagcgcccgt
    89281 tgtacggctt catcgagatc gaccgtctcg ccaatttgcc cacaaggatt cgcagcgtga
    89341 tcctgtttat cgattgacgc accttcaact tgcaggaaaa atcctttctc atttttactc
    89401 aacaattcaa tggctttgtc ggtcatctgc gccaaggttg gtacactgtc attacgttgc
    89461 ggattaggcg tacaggtgac tgcgggctta tcgatattgc cgtggtacgt tgctttcggt
    89521 cctagccagc gcactggcat attgccgtca gcaaacagtc caagcagagg tttttgctga
    89581 ttcgcttccg tcaccgaatt cagtgaggca gcatcgctga ccaactgata accacgcgcc
    89641 tgtgcctgtt cacgcagcgt ttttccctgc cattcaccgg cggttgccgt ttcagcaaag
    89701 gtttttgcgc caccgccaag cgtaacgtcg gcacgagcgt taagcagctg ttcggtaatc
    89761 gatccttttc cgcctttttc cagagcgtta cccggacatt tttcactggt cgcgctcgga
    89821 ccgtagcatt tgcgcgaggt cacatgcgcc accagcgcag cgggcgtggc atcctgcaac
    89881 tctgcggtag aaacgttacc ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt
    89941 gggtgatctt tttcgtgaat atcgacgccc agcgcgccgt tataggtttt gacaccggtt
    90001 gaccaggcgg ttgctgatgc agccgaatcg gtgacgtagt ccggtttgcc ggttttttta
    90061 ttcagcgcat agtgagtgta ttgcccggta agcggtaagg catcgatacc tttaaaaaag
    90121 ccgcccgcac cttcggcata attacgtgcg gctgtaattt ccgaatcccc catcccatcg
    90181 ccaatcagca aaataatatt ttttgcaggt ttatcgctaa gagaatcacg cagagcggca
    90241 gtctgatcac ccgttaaacg gcgagcaccg ccggatgcag taatatcgcc ctgagcagcc
    90301 cggttttcca gaacaggcat ttctggtgtc cgggcttttg tcacaggggt aaacagtaac
    90361 gataagagtg ccagtgcaat agtgctttgt ttcactttat tttctccatg tacaaataca
    90421 ttaaaaaata aaaacaaagc gactataagt ctcggccgtg acaactttat gacagctgtt
    90481 gaaaagatta actttttact gacgaggatg cttcaataac ttctttacgt aatcgcgcag
    90541 cagctccgta tcgtcgtcag gaatgctggc atcgggcttt acctcgtaca gcgccccctc
    90601 tacctgatca atcaagcgct gttggtcatt ttgcgccata ttgcgaagca ttgcagtgac
    90661 gataatctcc aaagcttcta cctgcgcaca cagttctttc gactcttctt ctttttgggc
    90721 aagcttaaat aacaactcag caatgagatt tttcatgtct gtatttcctt atccaaagta
    90781 tggagaagtt aacattatgt ttcacaactt catagtgtct ggtagtatta tttttatgac
    90841 aaaggaaata ctttgcgcaa atattttttg tttgattacc agcgaaacgt ttctcttatt
    90901 taaataatta cccacacaaa atataggctt ttaattatca acctttatta taaataaaag
    90961 ttatgaaata tattctcttc gttaacttat aatttagaga aaaaaactca ccgcatggcc
    91021 atttaagcca ggaataaatt agcttcccgt tcatcatttt tgtgatcttg cgcacatctt
    91081 tgagataacg cactttcttc accagtacgt tgcacgcttt gtcagcagaa gattgcaaca
    91141 atctttcaga aggccgcgaa tcccccgttt tgtcgtggta atgtatgcac tttgtgttag
    91201 acgggatagg tttttaagat ggaaaaactg cgggtaggaa tcgtttttgg tggtaaatca
    91261 gcggaacatg aagtgtctct gcaatcggca aaaaacattg tcgatgccat tgataaaagt
    91321 cgcttcgacg ttgtgctgct gggcattgat aaacaagggc aatggcacgt cagcgatgcc
    91381 agcaattatc tgctaaatgc agacgatcct gcccatattg cgttacgccc ttcggcgacc
    91441 agccttgcgc aggtgccagg taaacatgag catcagctta tcgacgcgca aaacggtcag
    91501 ccgttgccga cggtggatgt cattttcccg attgtccacg gtacgctggg cgaagatggt
    91561 tccttgcagg gaatgctgcg ggtcgccaat ttaccgttcg taggttctga tgttctggct
    91621 tcagcagcct gtatggataa agatgttacc aaacgtctgc tgcgcgatgc cgggctgaac
    91681 attgcgccat ttattaccct gacgcgcgcc aatcgtcaca acattagttt tgccgaagtg
    91741 gagtctaaac tggggttacc actgtttgta aaaccggcta atcagggctc ttctgttggt
    91801 gtcagcaaag taaccagtga agaacagtac gcaattgccg tcgatctggc gttcgaattc
    91861 gatcataaag tgatcgttga gcaagggatc aaaggtcgtg agatcgaatg cgcagttctg
    91921 ggcaacgaca acccgcaagc cagcacctgt ggcgagatcg tactcaccag cgacttctat
    91981 gcctacgaca ccaagtacat tgacgaagat ggcgcgaaag tggtagttcc ggcagccatt
    92041 gcgccagaaa tcaacgataa gatccgggcg attgccgttc aggcttatca aacgttggga
    92101 tgcgcaggca tggcgcgtgt agacgtgttt ttaaccccag aaaacgaagt ggtgatcaac
    92161 gagatcaaca cactgcctgg cttcaccaac atcagtatgt atccgaagct gtggcaagcc
    92221 agcggtctgg gttacaccga tctgatcaca cgtttgattg agctggcgct ggagcgtcac
    92281 gctgcggata acgcattgaa aaccacaatg taatatttta acgcccgata atattcttat
    92341 tgggcgtttt cagtttcttc atcgcgacga cgaataatca gccccgccag ccagaaacta
    92401 atcacccacg tcaccagacc gacggcatag gtctgccaac cttttgcttc aaatcccagt
    92461 aaccccacca cgccattaag aatgaatata aggccaatgg cgaacgcata gtagtgccag
    92521 tcacggcgga tttttacagg cagattcatg gcagctccgt taacgaaaaa caacatcctt
    92581 gcatattctg taacaaccgt ctgtggtgta gacaacattt tcgtaattgt taataaattt
    92641 aacttaaggt tgccattttg tgactcacaa cgaagattca cgcttcggga atattcccaa
    92701 catcaattta ccatgattct gatattgaca atctaaatga cctgtcagac ttgccataag
    92761 ccgctggtat cacaccagaa gagaagcaga tgacttccgg aggtttttat ggctgatttc
    92821 accctgtcaa aatcgctgtt tagcggaaaa tatcgcaatg cctcttcaac gcctggcaac
    92881 attgcctatg cgttgtttgt gctgttttgc ttttgggctg gggcgcaatt gctgaacctg
    92941 ttagtgcatg cgcccggcgt ctatgagcgt ttaatgcagg tccaggaaac aggtcgccca
    93001 cgggtggaaa ttggtttagg tgtcggcacc attttcgggc tgatcccgtt tttagtaggc
    93061 tgcctcattt ttgcagtggt ggcgctatgg ctgcactggc gacatcgccg ccagtaagat
    93121 ttacgttgcc agatacggcc taaaacgcct tatctggcat gacattcctg tttgatcact
    93181 gtttcataca acgcgcccgc cgttcgtcta cgcgcttcgc aaaccaggcc gtagtcaggt
    93241 tgcgggtgat tttcgggctt tccagttgaa tgccaggcaa catttcgcgg ggtagtgatt
    93301 tacccgtttt cgcttcggca agttgataaa ctttcttgta cagcgccgtc tcctcaaaag
    93361 agaagctgtc acctttctct aactgacggc gaatttcgct gtcgctcatc cccagttttc
    93421 ccgccagttt gcgcgttgcc agttcggttt tcccgggttc tttactgtcg taacgaatta
    93481 aatcaccgtc cagtgccagc ttcacgccgc tggccttact gactgcgttc tgaaacgcgg
    93541 cattacggct ggcgtaccag ccagcgttaa aatcagcaaa gcggtatatc ggtgcgctat
    93601 agctggcggg ataattcagt aaatggtaag taccaaacca caacccgccg cggcgactga
    93661 agacttcctg acgaactgta ccgtccattt tccacggata ccctttggta tgctgttcgg
    93721 caaaagcaat gctgacctgc atcggcccac cggtgcgcac aggattaagc gaaccaaaca
    93781 gcgtctgccc cattggtacc atgctgatca gatcgtcaaa aatcgcgctc aattgctttt
    93841 ctgtacgcac cgaatctaac cgttcgctat aacttttgcc gtttggcgat ttgattttta
    93901 gcgcggtatg aaccagaaaa gcaggaatgt gcatccgttc agcacgacgg tcaatctctt
    93961 gccaggcgat cttgcttaag cccggaacgg ccggatctgc ctgataattt gactcctgct
    94021 gcgccaccgc cagcaccgag cagacgtttt ccagcgtcgg tgccagcccc tgactttcaa
    94081 aggtggtggc gagatctttc gcccaggcat cgcggtcttt cacgctcgca ggcatcttct
    94141 gccgcacgac gctcgctaca tctatcgctt tttcgccctt tttcaacggt tgcggagcct
    94201 gactactaca ccccgctaaa accagaaccg ccaacaatga cagcgaagat agatgattga
    94261 cgcgcgacat tacttctcct ttttagctca aggtatgggt tacttcctga attttgtcac
    94321 catccagctc atgttcaaag ctgcgcagac gtttgtagat agacatcaac tcaaccagtg
    94381 tggtccatga gttaatcagg tactggaaag caccgcgaac ctgaccaaaa acgttggtaa
    94441 tctgcgtcat caggccgagc gtaatcgtac cggcaacaat tgacggaaac agcaagaaca
    94501 aaccgaaaac gttatcgacc tgcaaataga gaatgcgggc gatgttgaaa tacatatagt
    94561 gaaaatagag gcgaaaatag tttttccgta cggcgctaaa cagctcgcgt accgtaggcg
    94621 gcgtcgcgcg cgtggcatcg tcttcaccat aaaccagctc tttacggtag gcggcctcta
    94681 ctcgctggtt tttaaactcc agccccggca gtttgatccc taccactgcc agcaatccgg
    94741 tccccatcag cgaccagacg atagcggcaa tcaccagacc atacggaatg tgcccgataa
    94801 tcggcagctc cggcacatgc gcggagagcg ttaccagcac cggcagaaag gcgatcaacg
    94861 tcatgatggc gttgataaaa ctgacgccca tattctccag cgttgaagca aaacgcatgg
    94921 tgtcttcctg cacacgctgt gcggcacctt cgatatgacg cagttgttgc cagttcgcca
    94981 tgtaatattc gttcatcgcc gtacgccagc ggaacacata gtgactgaca aagaagttgt
    95041 tcagtacact gatcaccacc gcgatcagcg caatccccag aaagacgccc acttcgcggt
    95101 aaaattgttc gatagtgact ttatgcggcg aacttagcgc ggtttgaatc agatcataga
    95161 acggtgcata ccaggcgttg acggcgaccc cgacttccac caaaaaccag gtgacgaaga
    95221 tgatcagtgc agtaccgagt attgaccagt attgccaacg gtgcgggctg tagataaacc
    95281 agaaaaatgc aaaaagtcct acgcaaacaa tgtagtaagc gtaaaaaatc aggaaatcca
    95341 acgaccagaa acgcgcggcg ctaatcggga tctgcccgga agcgccggtg atacgcgcca
    95401 cccagtcacc cccaccggct tgccagaaga taacggcaat caatgcccaa acaaaggccg
    95461 agagaaaaaa cgttcccggc tttgggaaaa aagacttaaa catatgcact cctgctaact
    95521 tcttatcgtt ttaacaactg ctgtgtatta accgcatgac cgctacaaag cgtcagccaa
    95581 atgaaaccgc ccattgtgac ccatagcaag gcgcttagtt cgccagcctg accataagaa
    95641 ttgtttcgac ggatttcaca aaccaaagat tgtttacata agcggattaa cacgcgcagg
    95701 atcacaaatg caggcattgg tgagcaggaa aaagatcgtg tttacgcgcc gttacgtttt
    95761 taacactatt attgcactaa ttcgcccttt gcaatccatc aatgagtagt ataaatacgc
    95821 tcagttacct tcattcaatc tatggacacc accgttgaaa cgtagtctgc ttttttctgc
    95881 cgtgctgtgt gcggcgtcat tgacctccgt ccatgcggcg cagccgatca ctgaaccgga
    95941 gtttgcctct gatattgtcg atcgttatgc cgatcatatt ttttacggca gcggtgccac
    96001 ggggatggcg ctggttgtta tcgacggtaa ccagcgcgtc tttcgcagtt atggcgaaac
    96061 gcgacctggt aataacgttc gcccgcagct ggattcggtc gtgcgtattg cttccctcac
    96121 caagctaatg accagtgaaa tgctggtgaa attgctcgac caggggaccg tgaagttaaa
    96181 cgatccgcta agtaaatatg ctccgccagg cgcacgcgtg ccaacctaca atgggacgcc
    96241 cattacgctg gtcaatctgg caacccatac cagcgcgctg ccccgtgaac agcccggtgg
    96301 cgcggcacat cgtccggtgt ttgtctggcc aacgcgcgag caacgctgga aatacctttc
    96361 tacggcgaag ctgaaagccg cgccaggcag tcaggcggcg tactctaacc ttgcgttcga
    96421 cctgctggct gatgcactgg cgaatgcgtc cggcaagcct tatacccagt tgtttgaaga
    96481 gcagattacc cgtccattgg ggatgaaaga caccacctac accccctcac cggatcagtg
    96541 ccgccgttta atggttgcag agcgcggtgc cagtccatgc aataacacgc tggcggcaat
    96601 tggcagcggc ggcgtctatt ccacgcctgg cgatatgatg cgctggatgc agcagtattt
    96661 gtcatctgat ttttatcagc gcagtaacca ggcagatcgc atgcagacgt tgatttatca
    96721 gcgcgcgcag tttacgaaag tgattggcat ggatgtcccc ggcaaagcgg atgcgcttgg
    96781 tttaggctgg gtatatatgg tcccgaaaga gggtcgtccg gggattattc agaagacagg
    96841 aggtggtggc ggtttcatta cctatatggc gatgatcccg cagaagaata ttggcgcgtt
    96901 tgtggttgtc actcgttcgc cgttgacgcg ctttaaaaat atgagcgatg gcatcaatga
    96961 cctggtgacc gagctaagcg ggaataaacc gctggttatc cccgcgtcct gattaatact
    97021 ctgaaggcaa acggttgata gcgaccagtt tgcctttatt catttcgata tagccgcctt
    97081 tacgcaaagc agcgagaact tctgcgacga cagaacgcga tatgcgtgta cgttgatgga
    97141 tataattcat cacaccaata cgtgatcgca attcttcatt ccagtcaatc attgataata
    97201 atgttgcgcg gatttgttcg taggaattat gcccaatgag ctgcacgtcg cgtaattcca
    97261 gaattctatt ttgccaggct aaccagtaaa aagcgtctcg ccagagttga ttttgttcaa
    97321 taagcgtaat ggtttgtttg gctggtagat gatatcccgt acaatttcct tctgatatta
    97381 atttgtatgg tatatcgttt ttcattaaac catcagccag ccccataata taaggagcct
    97441 gggtaatacc gataagtacg ttttcttctc tacgcagaga gataactccc tctaaaataa
    97501 cgaaagtatc ttcgttattg acatcactgg aaaatataac ttgcttttca ttattaaact
    97561 cgaagcgcgt accgtatctg gacaaacatt tatcgagctt accaaattcc tgaagaggtt
    97621 taactacaga taacatttgc gcgtcctttg cagtaatgcc cgtcaaatcc ttgacgggca
    97681 ttatttagat taaattacca gaatttcttc ggagtgaaga atattaccag gtatatttaa
    97741 cacccacgtt cgcggaccag tcttgatcta cgtcaccacc accgaggtag ttagcatcgg
    97801 tataggcgct gaagttcttg gtgaagctga actgagtacc cagaccaaca cgtaccgcag
    97861 acccttcagt accgttatcg atggaatcac cgttcacatc gttatcgttg ttagagtcgt
    97921 cgtagacgta agccagtttg aagtacggag tcagagcctg gtcttcgctg taggtgaagg
    97981 tataacctgc atctacaccc agttcataac gcatgctgtc gtaagactga ccgtcaactt
    98041 tcatgtcgtt gctcagctgg tagtcatcac cagactggaa cagaccagaa acgctgccgt
    98101 aaggagtcac gtaaccagca tcacccagtt tgaagtcgta accggctttc aaaccgaagc
    98161 cccaagcgtc ggagttggtg ctaccgtcaa cgtaagtacc gttgctcatg gttgcagaca
    98221 ggtcgttgtt gaagtgagag tagctcaagc taccatcaac aaagacgttg ttcgcgaagt
    98281 gagcagaaga gtagatgtag gcagtctggc tgtcttgatc cacctgacca gaacggtcat
    98341 tcatgtcacc tttagcgaag cctgcagccg caccgacgat ccacttagcg ttgttaccgt
    98401 caattttggt atcaacaccg accatgatgc cgttaacatc ctgatcatag ttgatggtgc
    98461 cgttgtcgcc gttgaagtta ccaccgaagt agcttaccca tgcgccgccg ttatcagcca
    98521 ggccatgacg agagttggtc agacgagtac caacggtgtc ttgttccagg ttccagatat
    98581 tggtgttcgc agacgggatg ctcagcgcca tgttagcgta gtcggtcagc tccatctgtt
    98641 gcagaacaac ggtgttaccg cgctgttcag cctgataggt gtatgcaccc aggtcagctt
    98701 tgttagcagc agagaaggtc gcgttggtgt tgacgtcgtt tacgtagata atttctttat
    98761 ctttgtaatc agcgatagcg ccagcgcctg ttgcgttgtc gatacgaact ttgtagttac
    98821 cggttgcagc aacaacgttg tcaacagagc tgttatccag ttcgtcgccg ttgttcatgt
    98881 caccgttacc gttgatagtc agatgaccat cagagttcat agcaacaata ccgtagccgt
    98941 agttagactt ggaagtatcc caggtacggt cgttcaccaa atcagcgttc agaacataat
    99001 cgctgctgtg gatatcgaat acaccagaca ctacgttgcc gttatggtca gcgatgttgc
    99061 cgctggtcag ttccaaagta tcagtctgga acggattatc atcgaactgg tctacattaa
    99121 tatccagtaa accaccgtta gtcacagtta ttgtatttgc taacagcgac ggagtgtaat
    99181 aaccattata gatacttgaa ttgtcatcac tagacaacgc tacagtactg tagctatcaa
    99241 ctgtcagatg atcagttcca acataacctt catctccaat gttcaaagaa gaaccattgg
    99301 tcagagcgat gctatcagaa atcaagctag attcagacac attaacctga gagccattat
    99361 tcacagctaa agtatcaatg tttgaagcgt taacggtatt ccattcagaa ccattattca
    99421 gagtgacgtt aaacacaccg ctctggaata cttgatcatc aatcgtacga ccccaagcat
    99481 ttgcttctgt tacaacgccc ggatacaggc tattacctgt aacatcatac cagtcagtcg
    99541 aagcagtaga ttcatcatca acatttgctg atgtagtagc tgagcctacc cacttagaac
    99601 cattatccag agtgatattc agttcatcaa cattaagtga gtcatcagcc cagccaccat
    99661 tggtgtccaa gtacgccatc ggcattactg tcatgacctt gaggatagaa gtttgcgttg
    99721 aaagtacttg cgaaaacaac atcacccagc agagtagaat tgctgaaata aacattgttt
    99781 ttcatggagt aatttgaagc cgcactatca ctgaaataca gagctacatc attagcttcg
    99841 ccgttaccag agtaatcact aggttccgca gagttaccat agaaaccact gtcttccaga
    99901 gtagttacag aacctgaagt cactgtggaa tcggtaactg agatattgtt atcccaagta
    99961 tgagattcag tagtgtaagt attgtttacg gtattaccct gagtcaggct gataccagca
   100021 acgcgggagt tgttagaaat attgatgtta ctttcgacat caagattcac tgccagccca
   100081 aggccaacac caccgtttgc aaacaaaccg gcgtattctt caccttcacc agctggggta
   100141 tggaaggttt cgttagtata tttggtaacg tcaccgtcca gatatgaatc agtgaagtat
   100201 aaaccttcat aatcgtcatc aattgtagaa ttagcaatat ttaaagtgaa tacatcacca
   100261 tcatgccaat tatcatcaat tacacgatca gtaccgtatt cagagaactg atagacataa
   100321 tgaccatcac tgctttcacc cataggcagc attgaagtga ttgaaccatg aatcagggag
   100381 ttgctgatgt tcaactggtt tgttgttcca tcagtaaagt tgatcctacc cacgtaatat
   100441 ggacacaggc ctaagcgagg ttcttgtttt caaattgttc cggactgagg ccgccacaca
   100501 aactgtgccg ccgccaccga ttgtaatcac attcgatata attaaatacc gttgcccgca
   100561 ttatttcccg gctgataaag tgttctccat ggatacattc cactttcagc gaatgaaaga
   100621 agctttccac gcaggcatta tcgtagcagc aaccttt
//
LOCUS       DAZFJF010000003        96019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFJF010000003 DAZFJF010000000
VERSION     DAZFJF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.003"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..567
                     /gene="yfgH"
                     /locus_tag="ACHKAU_000203"
     CDS             49..567
                     /gene="yfgH"
                     /locus_tag="ACHKAU_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5529341.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            583..1122
                     /gene="yfgI"
                     /locus_tag="ACHKAU_000204"
     CDS             583..1122
                     /gene="yfgI"
                     /locus_tag="ACHKAU_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO5529342.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(1217..2794)
                     /gene="guaA"
                     /locus_tag="ACHKAU_000205"
     CDS             complement(1217..2794)
                     /gene="guaA"
                     /locus_tag="ACHKAU_000205"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO5529343.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2863..4329)
                     /gene="guaB"
                     /locus_tag="ACHKAU_000206"
     CDS             complement(2863..4329)
                     /gene="guaB"
                     /locus_tag="ACHKAU_000206"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO5529344.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4491..5861
                     /gene="xseA"
                     /locus_tag="ACHKAU_000207"
     CDS             4491..5861
                     /gene="xseA"
                     /locus_tag="ACHKAU_000207"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO5529345.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5858..6073)
                     /gene="yfgJ"
                     /locus_tag="ACHKAU_000208"
     CDS             complement(5858..6073)
                     /gene="yfgJ"
                     /locus_tag="ACHKAU_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5529346.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6142..7614)
                     /gene="der"
                     /locus_tag="ACHKAU_000209"
     CDS             complement(6142..7614)
                     /gene="der"
                     /locus_tag="ACHKAU_000209"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO5529347.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="ACHKAU_000210"
     CDS             complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="ACHKAU_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO5529348.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="ACHKAU_000211"
     CDS             complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="ACHKAU_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO5529349.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="ACHKAU_000212"
     CDS             complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="ACHKAU_000212"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO5529350.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="ACHKAU_000213"
                     /gene_synonym="gcpE"
     CDS             complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="ACHKAU_000213"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO5529351.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="ACHKAU_000214"
     CDS             complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="ACHKAU_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO5529352.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13387..14541)
                     /gene="rlmN"
                     /locus_tag="ACHKAU_000215"
     CDS             complement(13387..14541)
                     /gene="rlmN"
                     /locus_tag="ACHKAU_000215"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO5529353.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="ACHKAU_000216"
     CDS             complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="ACHKAU_000216"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO5529354.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15271..17583)
                     /gene="pbpC"
                     /locus_tag="ACHKAU_000217"
     CDS             complement(15271..17583)
                     /gene="pbpC"
                     /locus_tag="ACHKAU_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO5529355.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17584..22545)
                     /gene="yfhM"
                     /locus_tag="ACHKAU_000218"
     CDS             complement(17584..22545)
                     /gene="yfhM"
                     /locus_tag="ACHKAU_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO5529356.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPSETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22752..23597
                     /gene="sseA"
                     /locus_tag="ACHKAU_000219"
     CDS             22752..23597
                     /gene="sseA"
                     /locus_tag="ACHKAU_000219"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO5529357.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23933..24058
                     /gene="timP"
                     /locus_tag="ACHKAU_000220"
     CDS             23933..24058
                     /gene="timP"
                     /locus_tag="ACHKAU_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO5529358.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24090..24866)
                     /gene="sseB"
                     /locus_tag="ACHKAU_000221"
     CDS             complement(24090..24866)
                     /gene="sseB"
                     /locus_tag="ACHKAU_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO5529359.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25008..26291)
                     /gene="pepB"
                     /locus_tag="ACHKAU_000222"
     CDS             complement(25008..26291)
                     /gene="pepB"
                     /locus_tag="ACHKAU_000222"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO5529360.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26469..26669)
                     /gene="iscX"
                     /locus_tag="ACHKAU_000223"
     CDS             complement(26469..26669)
                     /gene="iscX"
                     /locus_tag="ACHKAU_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO5529361.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26681..27016)
                     /gene="fdx"
                     /locus_tag="ACHKAU_000224"
     CDS             complement(26681..27016)
                     /gene="fdx"
                     /locus_tag="ACHKAU_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO5529362.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27018..28868)
                     /gene="hscA"
                     /locus_tag="ACHKAU_000225"
     CDS             complement(27018..28868)
                     /gene="hscA"
                     /locus_tag="ACHKAU_000225"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO5529363.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28885..29400)
                     /gene="hscB"
                     /locus_tag="ACHKAU_000226"
     CDS             complement(28885..29400)
                     /gene="hscB"
                     /locus_tag="ACHKAU_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO5529364.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29496..29819)
                     /gene="iscA"
                     /locus_tag="ACHKAU_000227"
     CDS             complement(29496..29819)
                     /gene="iscA"
                     /locus_tag="ACHKAU_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO5529365.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29836..30222)
                     /gene="iscU"
                     /locus_tag="ACHKAU_000228"
     CDS             complement(29836..30222)
                     /gene="iscU"
                     /locus_tag="ACHKAU_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO5529366.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30250..31464)
                     /gene="iscS"
                     /locus_tag="ACHKAU_000229"
     CDS             complement(30250..31464)
                     /gene="iscS"
                     /locus_tag="ACHKAU_000229"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO5529367.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31705..32193)
                     /gene="iscR"
                     /locus_tag="ACHKAU_000230"
     CDS             complement(31705..32193)
                     /gene="iscR"
                     /locus_tag="ACHKAU_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO5529368.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32494..33231)
                     /gene="trmJ"
                     /locus_tag="ACHKAU_000231"
     CDS             complement(32494..33231)
                     /gene="trmJ"
                     /locus_tag="ACHKAU_000231"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO5529369.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33350..34153
                     /gene="suhB"
                     /locus_tag="ACHKAU_000232"
     CDS             33350..34153
                     /gene="suhB"
                     /locus_tag="ACHKAU_000232"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO5529370.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34298..35152
                     /gene="yfhR"
                     /locus_tag="ACHKAU_000233"
     CDS             34298..35152
                     /gene="yfhR"
                     /locus_tag="ACHKAU_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5529371.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35343..36623
                     /gene="csiE"
                     /locus_tag="ACHKAU_000234"
     CDS             35343..36623
                     /gene="csiE"
                     /locus_tag="ACHKAU_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO5529372.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36615..37754)
                     /gene="hcaT"
                     /locus_tag="ACHKAU_000235"
     CDS             complement(36615..37754)
                     /gene="hcaT"
                     /locus_tag="ACHKAU_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO5529373.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37914..38804)
                     /gene="hcaR"
                     /locus_tag="ACHKAU_000236"
     CDS             complement(37914..38804)
                     /gene="hcaR"
                     /locus_tag="ACHKAU_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO5529374.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38940..40301
                     /gene="hcaE"
                     /locus_tag="ACHKAU_000237"
     CDS             38940..40301
                     /gene="hcaE"
                     /locus_tag="ACHKAU_000237"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO5529375.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40298..40816
                     /gene="hcaF"
                     /locus_tag="ACHKAU_000238"
     CDS             40298..40816
                     /gene="hcaF"
                     /locus_tag="ACHKAU_000238"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO5529376.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40816..41136
                     /gene="hcaC"
                     /locus_tag="ACHKAU_000239"
     CDS             40816..41136
                     /gene="hcaC"
                     /locus_tag="ACHKAU_000239"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO5529377.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41133..41945
                     /gene="hcaB"
                     /locus_tag="ACHKAU_000240"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41133..41945
                     /gene="hcaB"
                     /locus_tag="ACHKAU_000240"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO5529378.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFMGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41955..43157
                     /gene="hcaD"
                     /locus_tag="ACHKAU_000241"
     CDS             41955..43157
                     /gene="hcaD"
                     /locus_tag="ACHKAU_000241"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO5529379.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43254..43676
                     /gene="yphA"
                     /locus_tag="ACHKAU_000242"
     CDS             43254..43676
                     /gene="yphA"
                     /locus_tag="ACHKAU_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5529380.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTTVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43724..44596)
                     /gene="yphB"
                     /locus_tag="ACHKAU_000243"
     CDS             complement(43724..44596)
                     /gene="yphB"
                     /locus_tag="ACHKAU_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5529381.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44608..45702)
                     /gene="yphC"
                     /locus_tag="ACHKAU_000244"
     CDS             complement(44608..45702)
                     /gene="yphC"
                     /locus_tag="ACHKAU_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5529382.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45735..46733)
                     /gene="yphD"
                     /locus_tag="ACHKAU_000245"
     CDS             complement(45735..46733)
                     /gene="yphD"
                     /locus_tag="ACHKAU_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5529383.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46758..48269)
                     /gene="yphE"
                     /locus_tag="ACHKAU_000246"
     CDS             complement(46758..48269)
                     /gene="yphE"
                     /locus_tag="ACHKAU_000246"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5529384.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48292..49275)
                     /gene="yphF"
                     /locus_tag="ACHKAU_000247"
     CDS             complement(48292..49275)
                     /gene="yphF"
                     /locus_tag="ACHKAU_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5529385.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49372..52653)
                     /gene="yphG"
                     /locus_tag="ACHKAU_000248"
     CDS             complement(49372..52653)
                     /gene="yphG"
                     /locus_tag="ACHKAU_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO5529386.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52771..53964
                     /gene="yphH"
                     /locus_tag="ACHKAU_000249"
     CDS             52771..53964
                     /gene="yphH"
                     /locus_tag="ACHKAU_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5529387.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54028..55281)
                     /gene="glyA"
                     /locus_tag="ACHKAU_000250"
     CDS             complement(54028..55281)
                     /gene="glyA"
                     /locus_tag="ACHKAU_000250"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO5529388.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55609..56799
                     /gene="hmpA"
                     /locus_tag="ACHKAU_000251"
     CDS             55609..56799
                     /gene="hmpA"
                     /locus_tag="ACHKAU_000251"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO5529389.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56844..57182)
                     /gene="glnB"
                     /locus_tag="ACHKAU_000252"
     CDS             complement(56844..57182)
                     /gene="glnB"
                     /locus_tag="ACHKAU_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO5529390.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57243..58577)
                     /gene="glrR"
                     /locus_tag="ACHKAU_000253"
     CDS             complement(57243..58577)
                     /gene="glrR"
                     /locus_tag="ACHKAU_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO5529391.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58567..59280)
                     /gene="qseG"
                     /locus_tag="ACHKAU_000254"
     CDS             complement(58567..59280)
                     /gene="qseG"
                     /locus_tag="ACHKAU_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO5529392.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59445..60872)
                     /gene="qseE"
                     /locus_tag="ACHKAU_000255"
     CDS             complement(59445..60872)
                     /gene="qseE"
                     /locus_tag="ACHKAU_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO5529393.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61430..65317)
                     /gene="purL"
                     /locus_tag="ACHKAU_000256"
                     /gene_synonym="purI"
     CDS             complement(61430..65317)
                     /gene="purL"
                     /locus_tag="ACHKAU_000256"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO5529394.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65575..67131
                     /gene="mltF"
                     /locus_tag="ACHKAU_000257"
     CDS             65575..67131
                     /gene="mltF"
                     /locus_tag="ACHKAU_000257"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO5529395.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67128..67631)
                     /gene="tadA"
                     /locus_tag="ACHKAU_000258"
     CDS             complement(67128..67631)
                     /gene="tadA"
                     /locus_tag="ACHKAU_000258"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO5529396.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67689..68324)
                     /gene="yfhb"
                     /locus_tag="ACHKAU_000259"
     CDS             complement(67689..68324)
                     /gene="yfhb"
                     /locus_tag="ACHKAU_000259"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO5529397.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68533..69381
                     /gene="yfhH"
                     /locus_tag="ACHKAU_000260"
     CDS             68533..69381
                     /gene="yfhH"
                     /locus_tag="ACHKAU_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5529398.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69437..69697
                     /gene="yfhL"
                     /locus_tag="ACHKAU_000261"
     CDS             69437..69697
                     /gene="yfhL"
                     /locus_tag="ACHKAU_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO5529399.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69891..69971)
                     /gene="shoB"
                     /locus_tag="ACHKAU_000262"
     CDS             complement(69891..69971)
                     /gene="shoB"
                     /locus_tag="ACHKAU_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO5529400.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70391..70771)
                     /gene="acpS"
                     /locus_tag="ACHKAU_000263"
     CDS             complement(70391..70771)
                     /gene="acpS"
                     /locus_tag="ACHKAU_000263"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5529401.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70771..71502)
                     /gene="pdxJ"
                     /locus_tag="ACHKAU_000264"
     CDS             complement(70771..71502)
                     /gene="pdxJ"
                     /locus_tag="ACHKAU_000264"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO5529402.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71514..72242)
                     /gene="recO"
                     /locus_tag="ACHKAU_000265"
     CDS             complement(71514..72242)
                     /gene="recO"
                     /locus_tag="ACHKAU_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO5529403.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72254..73159)
                     /gene="era"
                     /locus_tag="ACHKAU_000266"
     CDS             complement(72254..73159)
                     /gene="era"
                     /locus_tag="ACHKAU_000266"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO5529404.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73156..73836)
                     /gene="rnc"
                     /locus_tag="ACHKAU_000267"
     CDS             complement(73156..73836)
                     /gene="rnc"
                     /locus_tag="ACHKAU_000267"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO5529405.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(74109..75083)
                     /gene="lepB"
                     /locus_tag="ACHKAU_000268"
     CDS             complement(74109..75083)
                     /gene="lepB"
                     /locus_tag="ACHKAU_000268"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO5529406.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(75099..76898)
                     /gene="lepA"
                     /locus_tag="ACHKAU_000269"
     CDS             complement(75099..76898)
                     /gene="lepA"
                     /locus_tag="ACHKAU_000269"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO5529407.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(77096..77575)
                     /gene="rseC"
                     /locus_tag="ACHKAU_000270"
     CDS             complement(77096..77575)
                     /gene="rseC"
                     /locus_tag="ACHKAU_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO5529408.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77572..78528)
                     /gene="rseB"
                     /locus_tag="ACHKAU_000271"
     CDS             complement(77572..78528)
                     /gene="rseB"
                     /locus_tag="ACHKAU_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO5529409.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78528..79178)
                     /gene="rseA"
                     /locus_tag="ACHKAU_000272"
     CDS             complement(78528..79178)
                     /gene="rseA"
                     /locus_tag="ACHKAU_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO5529410.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79211..79786)
                     /gene="rpoE"
                     /locus_tag="ACHKAU_000273"
     CDS             complement(79211..79786)
                     /gene="rpoE"
                     /locus_tag="ACHKAU_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO5529411.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79783..79938)
                     /gene="rseD"
                     /locus_tag="ACHKAU_000274"
     CDS             complement(79783..79938)
                     /gene="rseD"
                     /locus_tag="ACHKAU_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO5529412.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80194..81816
                     /gene="nadB"
                     /locus_tag="ACHKAU_000275"
     CDS             80194..81816
                     /gene="nadB"
                     /locus_tag="ACHKAU_000275"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO5529413.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81801..82538)
                     /gene="trmN"
                     /locus_tag="ACHKAU_000276"
     CDS             complement(81801..82538)
                     /gene="trmN"
                     /locus_tag="ACHKAU_000276"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO5529414.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82670..84004
                     /gene="srmB"
                     /locus_tag="ACHKAU_000277"
     CDS             82670..84004
                     /gene="srmB"
                     /locus_tag="ACHKAU_000277"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO5529415.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(84213..85094)
                     /gene="yfiE"
                     /locus_tag="ACHKAU_000278"
     CDS             complement(84213..85094)
                     /gene="yfiE"
                     /locus_tag="ACHKAU_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5529416.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            85197..85784
                     /gene="eamB"
                     /locus_tag="ACHKAU_000279"
     CDS             85197..85784
                     /gene="eamB"
                     /locus_tag="ACHKAU_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO5529417.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(85840..86223)
                     /gene="grcA"
                     /locus_tag="ACHKAU_000280"
     CDS             complement(85840..86223)
                     /gene="grcA"
                     /locus_tag="ACHKAU_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO5529418.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86528..87217
                     /gene="ung"
                     /locus_tag="ACHKAU_000281"
     CDS             86528..87217
                     /gene="ung"
                     /locus_tag="ACHKAU_000281"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO5529419.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(87265..88302)
                     /gene="yfiF"
                     /locus_tag="ACHKAU_000282"
     CDS             complement(87265..88302)
                     /gene="yfiF"
                     /locus_tag="ACHKAU_000282"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5529420.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88509..88928
                     /gene="trxC"
                     /locus_tag="ACHKAU_000283"
     CDS             88509..88928
                     /gene="trxC"
                     /locus_tag="ACHKAU_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO5529421.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            88997..89695
                     /gene="tapT"
                     /locus_tag="ACHKAU_000284"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             88997..89695
                     /gene="tapT"
                     /locus_tag="ACHKAU_000284"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO5529422.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89727..92387
                     /gene="pat"
                     /locus_tag="ACHKAU_000285"
     CDS             89727..92387
                     /gene="pat"
                     /locus_tag="ACHKAU_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO5529423.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92501..93856
                     /gene="pssA"
                     /locus_tag="ACHKAU_000286"
     CDS             92501..93856
                     /gene="pssA"
                     /locus_tag="ACHKAU_000286"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO5529424.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93902..94225
                     /gene="yfiM"
                     /locus_tag="ACHKAU_000287"
     CDS             93902..94225
                     /gene="yfiM"
                     /locus_tag="ACHKAU_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO5529425.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(94222..95520)
                     /gene="kgtP"
                     /locus_tag="ACHKAU_000288"
     CDS             complement(94222..95520)
                     /gene="kgtP"
                     /locus_tag="ACHKAU_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO5529426.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(95843..95958)
                     /gene="rrf"
                     /locus_tag="ACHKAU_000289"
     rRNA            complement(95843..95958)
                     /gene="rrf"
                     /locus_tag="ACHKAU_000289"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        22884 a        26168 c        24150 g        22817 t
ORIGIN      
        1 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
       61 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
      121 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
      181 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
      241 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
      301 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
      361 gctgtaggcg cggcagtggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
      421 ttaacatata aggaaggcac caaagtgtat acctctaccc aggtgggtaa agagtgccag
      481 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
      541 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
      601 tgcgccatct tggcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
      661 tctgctgtag cagaagctga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
      721 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
      781 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
      841 ctggacgccg aagcctctgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
      901 agcttagaga ttcagaaaca aaaactggcg ctggcaaaag aagaagcccg cgttaagcga
      961 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
     1021 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaacgcgtg
     1081 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
     1141 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
     1201 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
     1261 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
     1321 aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
     1381 gagagacaac ccaatcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
     1441 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
     1501 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
     1561 cacgaacgcc aaggcctggt cccgggaacg ggtgacggta cagcatgtcg tacggcaggc
     1621 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
     1681 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
     1741 ctttaccggt tgcagaagcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
     1801 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgcc
     1861 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
     1921 cttctgccgg tacgtgaaca atgttaagac caaagtgatc gccaaacata tccagaacct
     1981 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
     2041 cgatagcgcg gtgcagcagc attgcggtta cggaggaatc cacaccacca gagaggccga
     2101 ggatgacttt atcgtcgcct acctgctcgc ggatgcgagc tacagcatcg tcgataattt
     2161 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
     2221 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
     2281 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
     2341 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
     2401 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct gtcgtttacg acttcaacct
     2461 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
     2521 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
     2581 gcgcacgcgg gctgttttct tcggtggtgc tttccgggcc gccggaaaga ataatgccgc
     2641 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
     2701 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtccagaa
     2761 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
     2821 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
     2881 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
     2941 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
     3001 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
     3061 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
     3121 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
     3181 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
     3241 catggaacct accatcaccg cgcttgcgcc agctgcgata gctttggcga tgtcgccgga
     3301 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
     3361 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
     3421 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
     3481 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
     3541 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
     3601 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
     3661 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
     3721 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
     3781 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
     3841 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
     3901 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
     3961 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
     4021 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacgacg
     4081 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
     4141 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
     4201 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
     4261 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
     4321 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
     4381 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
     4441 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
     4501 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
     4561 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
     4621 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
     4681 gcaacagcaa ccgtcgggtg accttccgtc cacagcatgg gcaacaagtt ttagttcgcg
     4741 ccaatattac gctctacgag ccgcgcggcg actaccagat aatcgttgag agtatgcagc
     4801 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
     4861 aaggattgtt cgatctgcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
     4921 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
     4981 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcgccggggc
     5041 aaactgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
     5101 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
     5161 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
     5221 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
     5281 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
     5341 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
     5401 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
     5461 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
     5521 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
     5581 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
     5641 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
     5701 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
     5761 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagtaaaaa
     5821 acatccagcc ggtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
     5881 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
     5941 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
     6001 cgcaggatgg gcaacgcgca tggtcgttat cctgatcaag cacatgctga cattgtggac
     6061 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
     6121 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
     6181 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
     6241 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
     6301 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
     6361 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
     6421 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
     6481 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
     6541 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
     6601 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
     6661 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
     6721 aataccttcg cgcgcatcaa tcactaacat caccacgttg gcatcttcaa tggcctgcaa
     6781 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
     6841 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
     6901 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
     6961 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
     7021 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
     7081 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
     7141 cacatgctcc agcagactta atacgccacg accgtgagac gcggcgatcg ggtagatttc
     7201 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
     7261 gtttgccacc aggaaggtcg gtttttcacg ggagcgcaga tgtttggcaa tcgcttcatc
     7321 tgccggcatc aggcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
     7381 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
     7441 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
     7501 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
     7561 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
     7621 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
     7681 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
     7741 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
     7801 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
     7861 gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac
     7921 cggagaagtc agcaggcgat gcaacagatc gctttgtgtc cacagcgtaa cgccgccatc
     7981 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
     8041 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
     8101 aagcgccgtc aggttaccat tataggccag cgcgaaaaca acgccgttaa caacgacggg
     8161 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
     8221 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
     8281 acccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
     8341 aggcatatcg aggttaactg tccatttgac agcgccgtca gcctcgttca gcgcttgtaa
     8401 ctgaccgtta ctggtgtgga ttaacaccag accgtcgctg accaccgggc gcgaaagtgc
     8461 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
     8521 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcagac cgccagaaag
     8581 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
     8641 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
     8701 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
     8761 agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
     8821 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
     8881 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
     8941 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
     9001 atgcactacg cgcaccttgc ttatcacctt tgctcagcaa tgcttcacca cgcaggtcgg
     9061 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
     9121 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
     9181 catcgctcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
     9241 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
     9301 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
     9361 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
     9421 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
     9481 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
     9541 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
     9601 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
     9661 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
     9721 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
     9781 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
     9841 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
     9901 acgttcgagg cccatcgcaa aaccgacagc cggtgttgca cgaccgccca gttgttccac
     9961 aagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgagac tgttagtcac
    10021 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    10081 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    10141 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    10201 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    10261 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    10321 cagcgaaccg atagaattca gctcaagagt tacgtgttcg gaaataccca gcgcgcgcca
    10381 ccagcgggcg gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    10441 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    10501 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    10561 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    10621 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    10681 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    10741 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    10801 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    10861 gaaccctcaa cgcttccctc aatgtttcgg gagctatgcg ggttcaatca tacacgggaa
    10921 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    10981 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    11041 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    11101 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    11161 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    11221 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttgat cccgcgcgaa
    11281 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    11341 gccagtgata cgcgcagcgt gtcgccgatg ccttcagaca gcagcagacc taaaccaatg
    11401 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    11461 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    11521 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atggcgcatg
    11581 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    11641 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    11701 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    11761 ccgtattccg ctactttcag tgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    11821 acgttaacct gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    11881 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    11941 acgtctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    12001 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    12061 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    12121 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    12181 actggatctg tactgcggct ggcgcaccaa ttttcagttt gtatggtgcc tggccggtta
    12241 agttcaaatt accgtcttta cgctgcatac cgctaaacaa ttttttaccg gtagcatcag
    12301 taacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    12361 gcgtagtcac gcctgcctga tcggttggca acggcgcagc gccatctggc gttgttgttg
    12421 ccgttggtgc cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    12481 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    12541 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    12601 tatttaacgg aacactctgc ccctgctcgc tattactgct cagttccgcc gaagattgat
    12661 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    12721 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccgtcacgtt
    12781 ttttgcggcg tttaccgagg gaaaaactct gcataggcgc aacttttgca gcccgtagtg
    12841 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    12901 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    12961 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    13021 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    13081 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    13141 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    13201 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    13261 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    13321 caacttaata cttcattcat aaaagattac gccacggtac ataaagtaac cgtggcgtaa
    13381 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    13441 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    13501 acgggtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    13561 gatacggctg ttcgagctgc gtccatacgg cgcgcccggg aaggggttcc acgggatcag
    13621 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    13681 gtcgttaacg tggtcaagca tcacatactc aatagtgaca cggccctgat tggcgttgga
    13741 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    13801 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    13861 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    13921 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    13981 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattgggcg
    14041 ctgaccggtg acttttgctg cgccgacgat tttcgccgca cgccacacct ggccaataat
    14101 ttccgatacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    14161 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    14221 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    14281 acgctgttct tcaaccactt ccggtgcgcg gatttccgcc acctctttca gtttgccgcg
    14341 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    14401 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    14461 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    14521 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    14581 gaggattgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    14641 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    14701 cgcgggcaca cttcgccttc gccaaagaaa taagcgattt cgcgagcggc agattcgacg
    14761 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    14821 ccagccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    14881 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    14941 tcaaagaacg gttttccatc gtgttcagca taaaagccac gtgcctgttc aacggtcagg
    15001 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    15061 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    15121 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    15181 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    15241 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    15301 ttgccccaca tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    15361 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    15421 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    15481 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatgacgg
    15541 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    15601 ttccagcggt agcggccaga catttatcat ttcttgtcgc gcttgcgggc aatcagcggc
    15661 aacacgtttg ccattttcat ccagccagat ggggaaacga atgccattaa tgccttcctg
    15721 ctccggcagt aatagagttg gcggctgact cccgtccagc agccaggttg ccaggcggcg
    15781 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    15841 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    15901 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    15961 gccgtccggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    16021 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    16081 ggcactatcc ggcaagggtt tcgcttcatc agccataatc cggcgaatga tccacgccgc
    16141 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    16201 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    16261 agcgccgccg agaatgagtg aaagattcgg cgcagcaccg ttgggcaaat ataacggcaa
    16321 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    16381 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    16441 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    16501 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    16561 tcctggcgat cggttcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    16621 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    16681 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    16741 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    16801 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    16861 ccttgactct tttacctgct cacgggacca cgcaccttgc acggccatcc gttcgagcac
    16921 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    16981 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    17041 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    17101 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    17161 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    17221 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    17281 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    17341 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    17401 gccgtcagca tcggcgaagc gccatagcgg cgtaccatcc tgcgccacga ccactcgtgc
    17461 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    17521 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    17581 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    17641 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    17701 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    17761 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    17821 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc
    17881 cagacccgca ggcagcagat ccacgaccaa cgcatccggc acgctgttac tggcttttac
    17941 ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    18001 atcagtacca agaatatgac gctcgatttg cagcacatcg ttcgccggta aaggtgcgga
    18061 ttgcggataa ccgctggcat ccacacgcag ccataacggc tgatcgccac tgtttctcac
    18121 ctgcaaggtg gcaagttgat cgctattcag attgctgttt agcgttttct cgcctgtcag
    18181 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    18241 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaatgcctg
    18301 ctgcgaaagc gtgttcagca aagtgttctg ctcatcgggt agcagtttat tttcttccag
    18361 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    18421 ctcatcacta tttctcggcg ttttcagcgc cagcacaatc gcttcttcac cacgcgttgc
    18481 atcacccatg gttttcagcg caacgccaag ttgcagcagc ggtaaaccag aagcggcatc
    18541 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    18601 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    18661 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    18721 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    18781 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    18841 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    18901 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    18961 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    19021 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    19081 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    19141 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    19201 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    19261 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    19321 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    19381 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    19441 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    19501 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    19561 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    19621 taccggcagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    19681 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    19741 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    19801 ccacggatct ggcgtgacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    19861 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    19921 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    19981 ttcatcgcca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    20041 tttatcgcca ggacgtacca ccagcgtact taaatacaga tcatgacgat tccaggtttt
    20101 atcgaccgga atcgtcagat ccagcccttg agcccgaaca tcaatctctt gccaccacag
    20161 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcgatatg
    20221 caacttaatg gtgtcgccag ggcgataact ggctttatcc agtttcagcg tgacacggtc
    20281 gggtcgcact gcgccgctac cgtcgctgtt gtcctgccag ctatagccag cccagaaacg
    20341 aacactactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    20401 cggaaaactg accttgccgg tttcgtccgc tttcagatcc agagtttgtt cattttcgat
    20461 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    20521 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    20581 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    20641 caccgtgctg tcagtacgat aatcgtaaac cgatttcgag gcgaactgcg gacggatccc
    20701 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    20761 cgattccagc aaactaccct ggaaaataac ctgtaatggg gaatgcgttt ccttccactg
    20821 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    20881 cgtgcgggaa agattttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    20941 agcttcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggtgc
    21001 accatacagg tagtacccca ccacggagaa tttcacttca tctttcggcg ttagcggggt
    21061 tttctcaccg gtcagattca gcgccatgcg ctctggcata aaatcttcga cgtggaaatc
    21121 ccacatccga tactggttat cgcccgtgtt agcgcgaata tgccacatac cggttgccgc
    21181 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgct
    21241 cctgagtacc tgcccatcgg gtttaatcac gtctaacttg atgggttgat tgggcaacgc
    21301 tttaccgtct gcatcacgca gcaaaccatt gaggattacc gtttcacttg ggcgataaag
    21361 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    21421 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    21481 cgccaacagt aatgccgcgt ttttatcatt ttccagctgc acatgcccct gagcgtcact
    21541 ggttgcctga gtcagagtct gccctttctc atttaataaa gagacttcaa ttccttgctg
    21601 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    21661 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    21721 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    21781 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    21841 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    21901 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    21961 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    22021 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    22081 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    22141 caaatcacgt ttcggttcga ggtggcgtaa acgtaactct ttaagattat ctgacagctc
    22201 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    22261 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    22321 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    22381 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    22441 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    22501 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    22561 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    22621 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    22681 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    22741 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    22801 atgacccgga aattcagatt attgatgccc gcatggcgtc gcctggacag gaagatcgta
    22861 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    22921 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    22981 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    23041 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    23101 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    23161 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    23221 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    23281 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    23341 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    23401 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    23461 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    23521 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    23581 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    23641 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    23701 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    23761 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    23821 gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc
    23881 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    23941 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa
    24001 ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc
    24061 ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttctgttt aaagtcacgc
    24121 aaaaaaccac cccagcgacg ttcatagaat ggcgcaatat gttcggtaat aaagtggcta
    24181 attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta
    24241 tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg
    24301 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    24361 ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    24421 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    24481 ggatttccct ctccaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc
    24541 ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg
    24601 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg
    24661 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta
    24721 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt
    24781 agagtacgga aaaagcccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    24841 tcaagttcgt tttttgtttc ggacatatga accacaattc attcaacgtt cggggcgcaa
    24901 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    24961 aatttgtagg ccggataagg cgtttacacc gcatccggca tattagttta cgccgttaac
    25021 agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc
    25081 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    25141 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    25201 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    25261 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    25321 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    25381 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    25441 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    25501 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    25561 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    25621 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    25681 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac cggtgcttct
    25741 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgc
    25801 ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    25861 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    25921 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    25981 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    26041 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    26101 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    26161 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    26221 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    26281 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    26341 ttttccgcta aatgattcgc gttgcaggaa ggcggcaagt gagtgaagcc ccaggagcat
    26401 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    26461 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg
    26521 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
    26581 gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc ttcgccaatt
    26641 tcgcggctat cggtccattt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    26701 tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc gcctggcagc
    26761 ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    26821 agctttccgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    26881 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    26941 tttcaccgct attagcttcc agaacagcgc catcagggca gagatcctga tgaggcaaaa
    27001 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    27061 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    27121 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    27181 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    27241 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    27301 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    27361 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    27421 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accgccagcc
    27481 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    27541 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    27601 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
    27661 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcaacag catttcgctg
    27721 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    27781 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    27841 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
    27901 cccgcgtctt taagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    27961 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    28021 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    28081 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    28141 tggtcgaaat catcaccacc gagcgcggaa tcaccgccgg tcgccagcac ttcaaacaca
    28201 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc cgccgaggtc ataaacggcg
    28261 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    28321 agtaagcgca ggacgtgaag gcccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    28381 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    28441 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    28501 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    28561 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    28621 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    28681 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    28741 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    28801 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    28861 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    28921 actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    28981 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    29041 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    29101 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    29161 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    29221 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    29281 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    29341 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga agtaatccat
    29401 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    29461 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    29521 ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag gccttctttt
    29581 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    29641 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    29701 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
    29761 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    29821 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    29881 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    29941 agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    30001 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    30061 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    30121 tgcacccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    30181 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    30241 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    30301 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    30361 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    30421 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    30481 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    30541 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    30601 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    30661 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat
    30721 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    30781 cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc
    30841 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    30901 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    30961 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    31021 aacaaggatg gtgtcgtcac gcatcgctgc ttcaagttct ttcaggtcga taatgccgtt
    31081 acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    31141 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
    31201 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    31261 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    31321 ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg ttccgtccat
    31381 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    31441 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    31501 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    31561 tctaatatct tgttatcttt aacctaactg tttcgggttt cagcaagttg acaaacttac
    31621 gaatctccgg gagcttacat aagtaagtga ccggagtgag tatgtgcagt caacgcagat
    31681 gcaacacgaa acaggacggt taaattaagc gcgtaactta acgtcgatcg cgtcttgtat
    31741 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
    31801 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
    31861 acgccacagc gcgtgggtca ggcatttatc gccgccctgg cagccgcctt taccctgaca
    31921 acgggtggca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
    31981 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
    32041 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
    32101 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
    32161 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
    32221 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
    32281 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
    32341 cattttttta tcgtggcgtt tgtacctgtt gtccttccag accatgggcg ggttgagggc
    32401 aattattcgc attagcgctg gcctcttccc ctcaccctaa ccctctcccc gaaggggcga
    32461 ggggaccgtc cgtgcattta tctggcttag ccctcaggcc ttattacctt tattctgctg
    32521 ctcaatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    32581 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    32641 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    32701 atacggcgtc tcttcatgct cgacctgctc gccgttttcc tgagttgcca gccaggccat
    32761 gcgcacttca taggcaataa cctgagccgc catcgccagg ttcagcgagc tgtattccgg
    32821 gttagccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    32881 gcgctcgcga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    32941 gccgcattcg cgcgggtcga gcatcggcca tggcagcgtg cgggagcgtg cgctggtgcc
    33001 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    33061 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg
    33121 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    33181 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    33241 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    33301 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    33361 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    33421 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    33481 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttacccac agcacaccat
    33541 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    33601 tccactggat ggtactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    33661 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    33721 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac
    33781 cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta
    33841 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    33901 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    33961 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcggg
    34021 cggcatcgtc agcgacttca ccggtggtca taactatatg ctgaccggta acatcgttgc
    34081 tggtaaccca cgtgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    34141 tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    34201 tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    34261 cgattaatgg atgataaaga agtcgcaagg aaaggttatg gcactgccag tgaacaaacg
    34321 cgttcccaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    34381 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    34441 ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc
    34501 ttcttcgacg ggccctgctg acaacgccat cgcaaccatc attcatgctc acggcaatgc
    34561 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    34621 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    34681 gctggacgat acgcaaagcg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    34741 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    34801 gggtgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    34861 catcgccaac cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    34921 aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    34981 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagagc caaaacggtt
    35041 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgccacg gcgatgttta
    35101 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    35161 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    35221 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    35281 tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    35341 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    35401 tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta
    35461 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    35521 gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag
    35581 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt
    35641 catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    35701 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    35761 tgcaaaaacg ctttgaatcg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    35821 tgctgcagca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    35881 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    35941 tccagcctgt accgcctgat gagccactgt ttatggcact acttttttcc atgttgcggg
    36001 ttcccgatcc actgcgggat gcgcatcagc gagacagaca attgcgtcag tctatcaaac
    36061 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat
    36121 gtgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    36181 ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc
    36241 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagtg
    36301 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    36361 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    36421 gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga
    36481 caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct
    36541 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    36601 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggcagc
    36661 gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    36721 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    36781 cggatgactt cgctaccctg gcgagcagca atataacgca tggcggccag gtggcagacc
    36841 gtgaaggtgc cgcaatgcag aatttgcacc actatcaacc acggcaacgc cgtagttgct
    36901 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgtgca
    36961 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    37021 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    37081 ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc
    37141 cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcgactt
    37201 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    37261 aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    37321 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    37381 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagtgg tgagaaaaag
    37441 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    37501 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    37561 tcgctgacgc ggggcgcgat gagcaaactc ccgaggaaac gggcaaccag acctgccccc
    37621 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    37681 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    37741 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    37801 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    37861 acaagcacta aaaaactgag atccgcgacg catgttgcaa aaataatgcg taattatgcc
    37921 gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    37981 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    38041 aaaacaacct gtccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    38101 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    38161 tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacacc
    38221 ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg cggtgatctc tttttcatgt
    38281 gctaaagggt gatcaaccgg taacacaacc actaatggtt cgtcaaaaag ctccagataa
    38341 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    38401 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    38461 ctgagacgaa acatcggtaa tacttttggc agtaaattca cttccgccga tggcacaaag
    38521 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    38581 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    38641 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    38701 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    38761 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    38821 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccagca
    38881 acatccttat ggcacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata
    38941 tgaccacacc ctcagatttg aacatttacc aactgattga tacccaaaat ggtcgggtca
    39001 ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc
    39061 gttgctggtt atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca
    39121 cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt
    39181 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    39241 gcgcctttac ctgtccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    39301 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg
    39361 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    39421 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    39481 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga
    39541 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    39601 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    39661 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    39721 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    39781 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    39841 tgcgcctggc gggtcataac aatatattcc ccacgctttc atggctaaac ggcactgcca
    39901 cactccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta
    39961 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    40021 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    40081 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    40141 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    40201 ctcgtggaat gtaccaacgc tgggctgatc ttctgagtag cgaaagctgg caggaagtgc
    40261 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    40321 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    40381 atttcgtgac tggctggcgc agctcgacga agagattcgt tacaccatgc gcaccacagt
    40441 taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa
    40501 tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    40561 agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    40621 catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg
    40681 cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa
    40741 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    40801 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    40861 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    40921 tatgccatta acgatcgttg cagccatggt aatgcgtcaa tgtcagaagg gtatctggaa
    40981 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    41041 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    41101 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    41161 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    41221 gcgcgcaggt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat
    41281 ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg
    41341 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca
    41401 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    41461 acgagctgtt taacgtcaat gttctcggtt acctgctggg cgcaaaagcc tgcgctccgg
    41521 cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc tggtatcctg
    41581 gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac
    41641 tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca
    41701 gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga
    41761 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    41821 tggggccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    41881 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc
    41941 tttaaggaag cacgatgaaa gaaaaaacca tcattattgt cggtggcggg caagcggcgg
    42001 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    42061 atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt
    42121 ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc
    42181 attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg
    42241 gcgaaagctg gcactgggat cagcttttta tagcaaccgg cgcggcagct cgaccgctgc
    42301 cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca
    42361 gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg
    42421 gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg
    42481 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    42541 accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    42601 aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg
    42661 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg
    42721 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    42781 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    42841 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc
    42901 cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc gatatgcgtg
    42961 gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc
    43021 aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg gcgtgagatt cgcccaattc
    43081 gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat gagaacatcg
    43141 cgcttaaatc actgtaacca ggataattag cgaatatctc aatgcctggg gcgtggcgag
    43201 gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg gttatgaaca
    43261 cactacgtta ttttgatttt ggagctgccc gccccgtttt gttattaatt gcccgtatcg
    43321 ccgtggtctt aattttcatt atttttggtt ttcccaaaat gatgggcttt gacggtacgg
    43381 tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct ggcagcgatt attgcggtag
    43441 ttatggaagt gcccgccgcg atattaatcg tgcttggctt tttcacccgt ccgctggcgg
    43501 tgctgtttat tttctacacg ctgggtacga cggtgattgg tcaccattac tgggatatga
    43561 ccggcgatgc ggttgggcca aatatgatta atttctggaa gaatgtcagt atcgctggcg
    43621 cattcctgct gctggcaatt accgggccgg gggcaatttc tctcgatcgg cgttaggaaa
    43681 gatgccggat gcggcgtgaa cgccttatcc ggcatttaat aaattacagc cactctaccc
    43741 gcaacgacat ctctgaagtt gttgattccc ctggtgctag cgcaatgaga tcaccccctt
    43801 ccgggcgatg atgatcatcc ggcgcatggc tcatcggttc cagacagaag aaatcaaacg
    43861 catatccttt atcaaacgca gggtcggaaa caaagatgaa ataacacggt gcaggtggcg
    43921 tcgtttccat gatgatggca taaccttcct gcggctgctc gatgcgggcc tgaccattcc
    43981 atccggcaaa accattgttc acccactggc gcggcaacgg cgcgggctgg ttaaaatcca
    44041 gttcctgcgg tagctgctcg caaaactcac ccgccagcca ctgctcccgc tccagccagt
    44101 aaccgctcgc ctgtgcctga atccgcgttt gcggcgacaa cggaaaataa ggatgccagc
    44161 cggtaccaaa tggcagcgtc tctgcccctt gattggtgac agagagcgtc accgtcagcg
    44221 tatccgccgt taaatgaaac gcctgactta cccgatagtg atagacaccg ctgcgatgtt
    44281 catacaccag acacaaacta tcatcgctgt gcgagacaca ttgccactcg cccagccagc
    44341 catcgccgtg tagatagtgc gcatcccact caacattcgg ttgcagttga tactcacgcc
    44401 cctgccagac aaaacgattg ccgctcaccc ggtttgcaaa tggcaccagc ggaaaacatg
    44461 aggcatcggt tgccacaccg ctttttttac caggacgtaa gagcggcgtc gtatcgcgcc
    44521 agaagccttc gattacgccg ccctggtcag aaacgtccag ctttagcgac ccgtgggata
    44581 aggtatagat ggtcatccgc taactcctta atccgggaag ttaatcacaa ctttcccgca
    44641 tttgccgctc gccatcagcg cataggcatc acctgcctgt tccagcgaga agcgatgggt
    44701 aatggcgtta cgcggccaca gtttccagtc cgtcagatca tgggcgcatt tttccatatg
    44761 gaacagactg gtcacccagg agccgataat ccgccgttga tggtgcatca gatcggcgct
    44821 gacctcgaac tccacttttc cggtttctcc gatgtaaacc acccgtcccc agtcagcggt
    44881 ggatcgcagt gccagcaagc gacctgcggc attaccagaa caatcgagcg caacatccgc
    44941 gccaccgtgg gtgagttcgg cgataatctg cggcagacct tcggtggttg ctaaatagcc
    45001 gtgatccatc acccctaact gttttgccat cgccagacgt tccggcagca tatcaacgcc
    45061 gatgatccgt tttgcaccgc gacctttcgc cagcatcatc gccatcatgc cgactggccc
    45121 cagaccgacc accagcacgt tatcactgcc ggaaacttcg ccgcgcaaaa ttccttcata
    45181 cgctgtacca acgccgcaac tgataaacgc accatcttcg tagctcagcg catccggcag
    45241 gaggatcaga tctttttctt ccgccagcag atattcggca tgaccgccgt cacgctgcca
    45301 gccgtaagcc gcttttcctt tgccagtaca agagatagga aagccgcggc ggcagttcgg
    45361 gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg tcgccctctt taaaatggcg
    45421 gcagccttgc cccatcgcca caatctgccc gcacggttca tgaccgttga taaatccctg
    45481 gtataacggt ttatcgggtg ccgccgccgt ggcacggtgt tgatgataga tatagtggac
    45541 atcgcttccg caaatcccgg aggatttcat tttgatcagt acctggttaa tccccggcgt
    45601 cggcaccgca acttcccgca gatcgacggt tgaatttcct ggtaaataag ctgccagcat
    45661 cgttttcata aatccctcat taacaatacg attaattttc atccctgccg cacccgcgcc
    45721 aggggcgtta aggtttagcg tttcgcttta ctgcttcgct gggtcagcaa gatattcgcc
    45781 agcaccgcca ccacgatgat gacgccgcgt accacctgct ggaaaaagga gttaataccg
    45841 agcagcacca gaccgttacc gattagcgta atcaccagca caccaagcaa tgtaccgaac
    45901 agggaaccgc gaccgccgga aagtgccgta ccgccgacca cgaccgcggc gatgacgtca
    45961 aactccagac cgtttgcggc acctgcgtta ccggaaccga ggcgtgccgc cagcaaaatg
    46021 ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa gaatgcgcac tcgacgaacg
    46081 ttaataccgc acaactgcgc cgccgtggca ttaccgccaa cggcaaaaac cgagcgcccg
    46141 aaggcggttt ttcggctaat gaacacaaac agcgcaaaca acaccatcat gatcagcgcg
    46201 gatactggca caccgagaaa ttgtccgccc agccagtcca gcacctcgtt ttcgtcaatc
    46261 ggcactggca gtgcgttggt cataaacagc cccattccgc gcagggcgct ccacagcccc
    46321 agtgtggcaa cgaaacttgg tacgttaaac acgccgcgca gcaccccggc gagcgttccc
    46381 atcagcgcgc ctaacagcaa caccagcaga cacgccaccg ccagcggaac ttcaaattgc
    46441 agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg gcccaacgct gacatcaatt
    46501 tcaccggaga taataatcag cgtcatcgcc caggcggcaa tcccaatggt ggcggcatcg
    46561 cgcagcacgt tcatctggtt attcaatgag ataaagcctg gcgcgttcag ggagaagacc
    46621 agataaagaa tggcaatcac caccagcaaa ccgatctcat taatatggcg actgacaaat
    46681 tgtttgagcg agacgctctt gccctgcggc aatggtaatg acgaagcaga catcttcagg
    46741 ttcctcggta aaattcatca gtgcacagac agaatggcgg acatcagctc atccacattg
    46801 accggagcgt gaaactcctg cgagaacgtg ccgtgctgta gtaacaggat gcggtcacag
    46861 accagcggca actcctccac ttcactggag ataaacacca cgctttttcc ttcggcagcc
    46921 agctcacgga caatacggta aatctgctgt ttggcttcga tatcgacgcc gcgcgttggc
    46981 tcgtcgagca acaaaatctg gctggcggca tagacccaac gaccgatcac cactttttgc
    47041 tgattgccgc cagaaagcgt gccgatgggc gtttcgctac tggcggcctt gaccgtcatc
    47101 cgctgcatca cctcttcggt caggcggcgg atggtggacc attgcagtac gccgttggcg
    47161 ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcccaacca gggaatgatc
    47221 cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat gtcgccgtaa
    47281 tcggggcgcg tgattttctc gccgttgata acaatttcgc cctgttcata ctcctccagc
    47341 ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca gcaggccagc aatgccaagc
    47401 acttcgccgc gacgtaacgt aaagctgata tcctccagct tgggcttatg gcgtaatgca
    47461 cggacttcca gcacggcctg atccacaatt tcctgagggg caaccggcgc aatatcaacg
    47521 tgatcacgcc ctagcattag cgacacaata tgatgcgtgg aggtgttttc gagcatcaca
    47581 tcgcccgcca cctgaccatc gcgcataacg gtggcacagg aggcaatgcg gcgaatttct
    47641 tccatccggt ggctgacata aatcaccgcc acgcccagtg ccgacatctt tttcaccgcg
    47701 atgatcacca gttcaacttc cgcactcgca agcgaactgg taggttcatc aagaatgacc
    47761 acgcgcggct cgcccttcat cacccgcgca atttccacca gttgcttttg cgccgggctt
    47821 agcgttgaaa caagttgttc agggctaacg tcaacgccca gcgcctgtaa gcaacgttgg
    47881 gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcggggcca ctgaccgagg
    47941 cagaggtttt ccgccaccgt cagtccctcc accagactta actcctgata aaccgcacga
    48001 acccccagtt cagcggcacg gcgagtcaac gtagcttcgt caccttccag tcgcgtctcg
    48061 ccaatccaga tatcaccgct atccgggcgt tcgctgccgg taagcattcg aatgagagtc
    48121 gatttgcccg cgccgttttt acctaacagc gcacgaactt cgcctttatt gagcgtgaag
    48181 ttaacgttat ccagcgcaac gacgccgggg taacgcttat ttcctgccac cacttttgct
    48241 accgggactg cctctgttgc cgtgaacata gggacctctg cgaatcagcg attagggcag
    48301 accatcaacg tgcgttgcca gccactgttt accatcttcc gttttggtat agagatcgat
    48361 tggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca atgtttgcgc
    48421 gaaaacagca ttgcccattt tcttaccgga aatatccact accgctttca gcacctgatt
    48481 gttttccagc tcctgagcaa tttcggttgt catatccgaa ccgaaaacag caatttttcc
    48541 ggcctgattt tgattacgta ccgcttttac cgcgccgagt gtcgcaccgc ccgactcccc
    48601 cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccaacgg aaatcgcttt
    48661 atctaaaaca gtcccttcct gattagcgac aatttgcgcg ccgggaacgc gggattttaa
    48721 tacttcttca aatcctttac gtcgctgcac acaaacttca aaggcttcgc aattgatgac
    48781 agcaattttc ggctggtcaa ttttattggc aataaaataa tcggcggcag cgttacccag
    48841 ttttttacca aattccagcg gatcgccgac cagatacgcc gagacatatt tatcgccccc
    48901 cttttgatta atacaggtgt tgtagcaaat caccggaata cccgcttcac tggcgcgacg
    48961 aacggtacgg ctactgccat tttcagacac tgccgataaa ataatggcat cgacattacg
    49021 cgccacgagg gtatcaacaa aggtactttc tttcgaaata tcaccctggg cgttagtttc
    49081 aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaacgccct gacgcactcc
    49141 agcgtaatat ccctgggtat caaggtatat tgcaccaatg gtcatttctt tatccgcagc
    49201 cctggcaaat agtgcgctac ctaatagcgt agccattaat aatagattac gggttgttct
    49261 cattttttta ggcatagtgc ctcctgtagg gtttttatta acaacggctt attctaatta
    49321 ttttgtgatg agcggcagcg cgtgccgctc aaatattaca gttaataggg tttagtgaac
    49381 gtagttaaaa ataaaaggca tcacggtggt gaataatgcc gctttcggcc aggccggatt
    49441 aatcgccgtc aattcagcgc gagcagattc atattgcgca acctccccga gtcccgcgga
    49501 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
    49561 cgacaacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
    49621 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct gttcacccag
    49681 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
    49741 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca tcgcttcagt
    49801 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
    49861 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
    49921 gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata actggttgag
    49981 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tacgcgtggc
    50041 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
    50101 ggtcatgtcg tcgcgtttca gcgcaatttc ctgattattt tccagacgcg ccagtcgttt
    50161 ctccggtgtg gctccactta acttatcaag caaatcccgt tcgaaaagca gacgtgcatc
    50221 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttg
    50281 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
    50341 tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt agtagaagca
    50401 ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
    50461 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
    50521 gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg tgcgctggca
    50581 gtccagtgcg ttcagcatct ctgccgccag ggtgggcatt ccccagttaa tcagttgccc
    50641 ggcggtcatc agggcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagagc
    50701 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
    50761 gcgcagcagt ttctcgcgct gcacacgcgc gttgtcctga cgaccactta acaccagcag
    50821 cagattatgc aggcaaagca cttcctgatt ggttgggcag gcgcgaagac tttgttggca
    50881 aaaatccaga ccagcgtcga agttaccatt acgcgccgcc agtcgtgcca ggccataata
    50941 gccaccggct ttgctgttgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
    51001 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa ctcgcctgtc cgcactgcgg
    51061 atttttgttc agcgcatgtg cgcgtttcag agcctgagtg gcatacgcca ccgcttgcgg
    51121 gaaatcggcg cggttatatt ccagcatcgc cagcgccagg ttacagcggt aatccagcgg
    51181 atccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt gatgatattg
    51241 ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt gtgctgccag
    51301 tggcgctttg gcgacgtccg gcaacggcaa ctcttgcggc tgatgttcct gataactcag
    51361 tacaatattg ccgtcggcat cggagagctc aatggtcagc ctttcaggat tgataccgtg
    51421 caacacgccc tggatggcgg tcgcaggcat cagtgccacg gcatcatcaa gtaacgcgtt
    51481 gcatttgccg atttcgcgga tcgccaggcg gtatccgttc aacggagaga tggcatacag
    51541 cccccactca atcccccgct tactacgctg gagttttatc accgcatcgc gggaggcatt
    51601 ttgcaccatg cccaaagaat gataaggcag gaaatactgc tcaaaacgct tctcttcgta
    51661 agcatcaagc caggtaaaat cgggctggtt atcggcaaaa ataccggtca tcagttcgat
    51721 atacggaccg ttattgtcgg tcagactctt atcccacgcc tggccaaatt cactgtgtcc
    51781 ccaactccac tgttttttac ctggcgcaat atggtggttg gcaacgtgca gcaaaccgcc
    51841 atcttcatcg tgacaccacg cgccgacaaa atcgtactgc gatttttcgg ccatatatga
    51901 ggttggaaca ggcacatttt tatagcgaga aatgtccact ccggcggagt agtccacttt
    51961 gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttgccgt gatcaaacac
    52021 cgcagtcaca tccggtggga aaacgctctg atgcccttcc ccccctttca ctgccgggtt
    52081 ggcccaccac aagaaatgac gcggcgtggc gttaccgtta tagacgcggc tggcgatttc
    52141 cagcgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac catgcatcgg
    52201 ctccgtttcg cctacccaca ccgtctgtgc accgtcttca tgggcttcga gggtgaaatc
    52261 aacgggcata aaggtggtcg ggcgatgatg ttgcggccag ttaaactcaa tcccaccgga
    52321 aatccacggc cccagcagcc ccaccagcgc aggtttaatg acttcattgt gataaacaaa
    52381 atcgcgctgt ttcactttat cccacgcgcg atgcacccga ccgcccagtt ccggcaggat
    52441 catcactttg atgtagtcgt tttccagcca caccgcctgc caggatttca gggttttctg
    52501 ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc cctgataaac
    52561 gcgattttcg aggaacatgg gatgtatatc ctgcggcccg gtttcatagg tcgggatctc
    52621 aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa caagacatac
    52681 tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg cggaataatc
    52741 acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa tcaacagatt
    52801 cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg cgccaataaa
    52861 tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat tttgcaggaa
    52921 ctggaaagcg aaaaacgggt ggtgaatatt gacgatgaaa gccagacgcg cgggcatagt
    52981 agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgcctgaa cgtgacgccc
    53041 accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggtga atttgagtat
    53101 ttacagattg atgcaccgac gccgcaggcg ctgctgtccg aaatcgaaaa atgctggcat
    53161 cgccaccgta aattgtggcc ggaccgcacc atcaatctgg cgctggcaat ccacggtcag
    53221 gttgatcctg tgaccggcgt gtcgcaaacc atgccgcaag cgccgtggac aacgccggtg
    53281 gaggtgaaat atctgctgga agagaagctc ggcattcggg tgatggtcga taatgactgc
    53341 gtgatgctgg cgctggcgga gaaatggcaa aataattcgc aggaacggga tttctgcgtg
    53401 atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat ttatcgcggc
    53461 agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg cgtcgtctgc
    53521 gactgtggac gttatggctg cctggaaacc gtcgcctcgt taagcgcatt aaaaaaacag
    53581 gcgcgggtat ggctaaaatc acaaccggtt agtactcaac ttgatcctga aaaactgact
    53641 acagcgcagt taatcgctgc ctggcaaagt ggagaaccgt ggatcaccag ctgggttgac
    53701 cgcagtgcca atgccattgg tttgagtctg tataacttcc tcaacatcct caatattaat
    53761 cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa tactattatt
    53821 cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa agcgacgcaa
    53881 attggcttcg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta tttgtatgtt
    53941 gaggcgcagt tacggcagat ttgatggcgc aataacgtag aaaggcttcc cgaaggaagc
    54001 cttttttcaa cagattaacc gtttcgctta tgcgtaaacc ggataacgtg cgcagatgtc
    54061 gagaacttta cctttgatgc gctcgataac agcttcatca ttgatgctgt ccagcacgtc
    54121 acacatccag ccagccagtt ctttcgcttc tgcttctttg aagccgcgac gcgtaattgc
    54181 cggagtaccc acgcgaatac cggaggtcac aaacgggctc ttcggatcgt tcggtacgct
    54241 gtttttgttg acggtgatgt tagcacggcc cagagcggcg tctgcttctt taccggtcag
    54301 gtttttatca accagatcaa ccaggaacag gtggttatca gtgccgccgg aaaccacttt
    54361 gtagccgcgc tcgaggaaca cttctaccat cgctttagcg tttttcgcga cctgctgctg
    54421 gtaagttttg aactcaggct ccatcgcttc tttcagagca accgctttac cggcgattac
    54481 gtgcatcaac ggaccgccct gaccaccagg gaaaacggca gagttcagtt ttttgtacag
    54541 ctcttcgcta ccacctttcg ccaggatcag gccgccgcgc ggacccgcca gggttttgtg
    54601 agtggtggta gtaacaacgt gagcatgagg aaccgggttc gggtagacgc cagcagcaac
    54661 caggcccgca acgtgcgcca tatcaacgaa caggtaagca ccgatgctgt cagcgatttc
    54721 acgcattttc gcccagtcca ccacgccgga atatgcagag aaaccaccga taatcatttt
    54781 cggcttgtgt tctttggctt gtttttccag atcggcgtag tcgatatgac cggtagcatc
    54841 gataccgtaa ggaacgatgt tgtacagttt accggagaag ttaaccggag aaccgtgagt
    54901 caggtgaccg ccatgcgcca ggttcatacc cagaacggta tcacctggtt ccagcagcgc
    54961 ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc tggacgttag cgtagtcagc
    55021 gccgaacagt tctttcgcac gatcgatcgc cagttgttca acgatatcaa catactcgca
    55081 accgccgtag tagcgtttgc ccggataacc ttcagcatat ttgttggtca gctgagaacc
    55141 ctgcgcctgc attacgcgcg ggctggtgta gttttcggag gcgatcagtt cgatgtgctc
    55201 ttcctgacgt actttttcct gctccatagc ctgccacagt tcggcatcat aatcggcaat
    55261 gttcatttca cgctttaaca tccgcatctc ctgactcagc taacaataaa atttttggcc
    55321 tttataggcg gtcctgttgg acaacggcga acagtataac cgaatcattg tgcgataaca
    55381 ggtcttgaca aaggaattta cgcaaacgat taccttcagg ctacgcaagg ctttggagaa
    55441 taaagagctt gcaaccggaa acggatttct tttcaggttt gtgatgcaaa tttttcactt
    55501 catcacattc tttctgaaaa acaccaaaga accatttaca ttgcagggct attttttata
    55561 agatgcattt gagatacatc aattaagatg caaaaaaagg aagaccatat gcttgacgct
    55621 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    55681 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    55741 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    55801 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    55861 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    55921 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    55981 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    56041 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    56101 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    56161 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    56221 cagtactctt tgactcgcaa accggatggc aaaggctatc gtattgcggt gaaacgcgaa
    56281 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    56341 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    56401 atctctgccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    56461 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgatgt tcacgccttt
    56521 gctgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    56581 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    56641 ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    56701 gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    56761 attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa
    56821 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    56881 cgaatgaccc gtgccacgtc gaagacgaag attttaccgt caccgatttt gccggtttgc
    56941 gccgtgcgaa taatggtatc aacacaggta tcgacaatgt cgtccggtac gacaatctca
    57001 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    57061 cctttctggc ggccaaaacc tttcacctca gtgaccgtca tgccggtaat gccgacttca
    57121 gccagtgctt cgcgaacatc gtccagcttg aagggtttaa taatcgcatc aatctttttc
    57181 atgctattcc ttgaaaaggt cgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    57241 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    57301 gtccggttgc gccccgccat tctcgccgcg tgtgtgacgt tgcctttggt gatttgcagc
    57361 agcttacgca aatagttgag ttcaaactga ttacgtgcct caacaaaggt tggcagcgcc
    57421 gtattttcac cctccagcgc ctgctctact aatgcatcgc taataaccgg agatgaggtc
    57481 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctagcg
    57541 gtcatcaggc gtttcatcgc atcagtggag aacgcgcgga caaacggttt atgtcgctct
    57601 gccgcctggc gcaacaggtg atttgccaga agcggaatgt cttctgtgcg ctctgccaga
    57661 gccggaattt tcaggctgac aacgttgagg cggtaataga ggtcttcacg gaattccccg
    57721 cgcgccatcg cttttggcag gtcgcggtga gtggcggaaa taatccgcac gtcgatatca
    57781 atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    57841 acctgcaacg gtgcgggcat atcgccaatc tcatcgagaa atagcgtacc gccttccgcc
    57901 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    57961 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    58021 ttgctattgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    58081 ctctggccgt taatcaaaac gctaacgtct gattgcgcca ccagccgcgc ctgttccagc
    58141 aaacgcagca tcagcgggct gcgggtgaca atcgcctcgc gccagcgttc atcggtggct
    58201 ggcgcggatt gttccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc
    58261 ttggtgagga aactgaacac gccctgctgt gttgcagcaa cggcatcggg aatagaacca
    58321 tgcgcggtaa gaataattac tggcattccc ggctgcactt tctgaatttc agcaaacagc
    58381 tgcataccgt ccatttcatc catccgcagg tcgctgatga ctaaatctac tttttcgcga
    58441 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacgctgta gccttcgctg
    58501 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    58561 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcaccctc
    58621 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    58681 atcagtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    58741 ttgctggcgc aatgtatcca gctcgctgtc gctggcctgc tggagcttgc cgtagcgttg
    58801 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagagg
    58861 gcgaacctgt gccgggatct cggtacttag cgcatcaatg cgcgcgacca gctggcggcg
    58921 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    58981 gctgccgtcg tcatattggc gagcttgctg acgtgactgc gcaggcatta aacgatcagc
    59041 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    59101 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    59161 ttctgctggc gtatcaatgg ctggcttatt gtgactctgc acgcaaccca gcaatgccag
    59221 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    59281 attcaccaga cttaatgcct atcccagtgg gcgtaattat tgcaggcagt ttggacatgg
    59341 acagcgcgga gaaaccggag cgtacacgtc gtacgtgagg atttcgagca ctgcccgggg
    59401 ccgaactggc aaataaaata gcctgatggg ataggctgtt agcattattt cgtgtttttc
    59461 gacgacggca attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    59521 tccccttgca tacggcaaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    59581 accgcccctt ttcgctggtg gcttccttga aaaaagggtt cgaagatcat ggcgcgttcc
    59641 tcttgcggaa tgggtgtgcc tgtattgatg acatcaatat aaacccgtgc accatgtaag
    59701 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    59761 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    59821 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    59881 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    59941 taatcaagca gttgttcgat cagtttttgc aaattgcggc tgctgctatc aagaatgctc
    60001 accacctctt tttgctctgg cgtaagcggc ccgacaacct ggtcagccag taattcagtg
    60061 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    60121 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    60181 gagcgtaact cgctcggtcc actgaacgaa acgctattgc ccagagaacg cccttccccc
    60241 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    60301 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    60361 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    60421 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    60481 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    60541 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    60601 cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag
    60661 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    60721 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    60781 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    60841 aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    60901 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    60961 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    61021 tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacatccc
    61081 gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc
    61141 aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt
    61201 cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa
    61261 cttattagat tacaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc
    61321 acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg
    61381 gttggtgctt aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact
    61441 gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat
    61501 gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc
    61561 gaccactttc agtcgtaacc gacgtaatac cgttcggaga accgttcggg ttagccgggt
    61621 aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt
    61681 ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcaccg tgagagacag
    61741 caatcggcat ttgcgagccc accatcccct gcaacagcag cgacgggctt tgggtcactt
    61801 caaccaggct gaaacgcgct tcaaagcgat cggaggtatt gcgcacaaaa cgtggccaca
    61861 actcactacc tgggatcagt tcacgcagat tagacatcat ctggcaaccg ttacataccc
    61921 ccagcgccag cgtttgcgga cggtggaaga aggttgcaaa ctcatcgcgt acacggtcat
    61981 tgaacaggat tgacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac
    62041 caccgcacgc gaccagggcg tggaaatctt ccaggcccgt gcgtccagcc agcaggtcac
    62101 tcatatgcac gtcgatagca tcaaagcctg cacggtggaa agctgccgcc atttcaacat
    62161 gcgagttcac gccctgctca cgcagtacgg caactttcgg gcgcgcgcca gtggcaataa
    62221 acggtgctgc cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg
    62281 cgtcgtttga tttcgcctgg tgctcctggt cagcacactc cgggttgtca cgcaggcgct
    62341 gcatctgcca ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata
    62401 cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat
    62461 ggacgcaatc agcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg
    62521 cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac
    62581 ccagagagcc gatatccgca tcaatgccac aatgaccagc aaaggccatt tccgccagtg
    62641 tgaccaacag accgccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca
    62701 gcgcctgaat cgcgtcatag aagcctttca gttgcgcgac atcgcgtaca tctgccggtt
    62761 tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt
    62821 tgcccaaatc aatcagcagc agtgcgttat cttcggtaga aagctgcggc gtgatggtgt
    62881 gacgtacatc ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt
    62941 cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg
    63001 ggatcgtcag gcccagcgcc ggacaaagct cttcgcccac ggctttaacg gcttcataca
    63061 ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc
    63121 gtttgatatc gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac
    63181 gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt
    63241 caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc
    63301 acggacccac catctgatcg cgcgctacca tgccagttac gctgcggtca ccgatggtca
    63361 ccaggaaagt tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa
    63421 tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg
    63481 tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa
    63541 aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg
    63601 gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca
    63661 gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt
    63721 cgcttagaat atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg
    63781 gcatcgcgtt agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat
    63841 caccaagctg ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt
    63901 cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg acctgacgcc atggaagacg
    63961 ccgctccgcc gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac
    64021 cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca
    64081 gcatgatcgg tttgtgataa ccgcgcagct cttcgccgtt gtggctgttc actttttctt
    64141 cataagtacg gaagtagcca ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcgc
    64201 cgcccagcgg gccttcggtc atgatgtcca gcgcggtgac aatgcgctca ggcttaccga
    64261 aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggaaacg gagaaaccaa
    64321 ccaaacccgc tttcggcttc gcgccgaggc cggtggcacc ttcatcgcgg atttcaccgc
    64381 cggaaccggt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga
    64441 ctttcatcag aatatgcgcc ggttcctgat ggaaatcgta gcggcccgtt tcgtggtcag
    64501 caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga
    64561 gaacgtaatc tggcgtggtt tcgaaggtgt ttttgatcat cttaaacagc gatttcggct
    64621 gttgctcacc gtcgataacc caatcggcgt taaaaatctt gtggcggcag tgctcggagt
    64681 tcgcctgggc gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag
    64741 catcctgcag atagtcaatt tcatcttccg ccagtgccag gccaagacgc aggttagcgt
    64801 cgattagcgc ctgacggccc tgccccagca aatcaacgct ggtgaccgga gtcggttgat
    64861 ggtgtgcaaa cagctgctct gcatcatcta aagcaaaaaa gaccgtttcc atcatgcggt
    64921 cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat
    64981 agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat
    65041 cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt
    65101 tgccttgcgg ggcgtggctg gcgagtgccg ggccatattt cagcaggcgt tcaagttgtg
    65161 cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa
    65221 tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg
    65281 ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt
    65341 cggtgtaccc aaggggggga aacgggcgtc attataaaga atctgatgcg ctgacgaaac
    65401 cgtttgcgtg gaaataaaat caccatcgtg aattagcaac gcgtgccgcc aatggctgta
    65461 ataagttgcc atctggcgca ggtttacgca aaatgccgct catttaagag taaacctttc
    65521 actattatta cgttttttca agctgggacg cgcacgacac agagaattaa ctaattgaaa
    65581 aaattaaaga ttaattatct gttcatcggc attctggcac tgctgctcgc ggtcgctctc
    65641 tggccatcca ttccctggtt tggtaaagcc gacaaccgta tcgccgccat tcaagcgcgg
    65701 ggagagttgc gtgtgagcac cattcatact cccctgactt ataacgaaat caacgggaaa
    65761 ccttttggcc tggattacga actggcgaaa cagtttgccg attacctcgg cgtaaaactg
    65821 aaagtgaccg tgcggcagaa tatcagccag ctgtttgacg accttgataa tggtaacgcc
    65881 gacctgctgg cggcaggact ggtctataac agtgagcggg taaaaaatta tcagcctggc
    65941 cctacctatt attccgtgtc acaacaactg gtttataaag tgggtcagta tcgcccacgt
    66001 acgctgggca acctgacggc ggagcaactc accgttgcac cgggtcatgt ggtggttaac
    66061 gatctccaga ccctgaaaga aacaaaattc ccggaattaa gctggaaggt agacgacaaa
    66121 aaaggctctg cggaattaat ggaagatgtc atcgaaggaa aactcgatta caccattgct
    66181 gattctgtcg ccatcagcct gtttcagcgc gttcacccgg agctcgccgt agcgctcgat
    66241 atcaccgatg aacaaccggt gacttggttt agcccgttag atggcgataa taccctttcc
    66301 gccgccctgc tcgacttctt caacgaaatg aatgaagacg gtacgctggc acgcattgaa
    66361 gagaaatacc tggggcatgg cgatgatttt gattacgtcg atacgcgcac atttttacgc
    66421 gccgtcgatg cggtactgcc gcagttaaag cccctgtttg agaaatacgc cgaagaaatt
    66481 gactggcgtt tgctggccgc tattgcttat caggaatcgc actgggatgc acaggccact
    66541 tcaccgacgg gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca aagcctcggc
    66601 attacggatc gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta tttgcaggat
    66661 atgatgagta aagtgccgga aagtgtgccg gagaacgagc ggatctggtt tgccctcgct
    66721 gcgtacaata tgggctatgc gcatatgctg gatgcccgcg ccctgacggc aaaaaccaaa
    66781 gggaatcctg acagttgggc tgacgtaaaa cagcgtctgc ctttacttag ccagaaaccc
    66841 tattacagca agctgactta cggctacgct cgtggacatg aagcctacgc ttatgtcgaa
    66901 aatattcgta agtatcagat tagcctggtg ggttatctgc aagagaaaga gaagcaggct
    66961 acagaagcgg cgatgcaact ggcgcaggat tatccggcgg tatcgcctac ggagttgggc
    67021 aaagagaaat ttccttttct ctcgtttctt tcccagtcgt catcaaacta tttgacccat
    67081 tctccctctc tgctgttttc caggaaaggg agtgaagaga aacaaaatta atccgtcgag
    67141 gattgcgctt ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa gtcactgagc
    67201 aacgccgcgc actcatccgc cagtattcct tccgtaattt ccactcggtg attcataccc
    67261 ggatgatgca gcacatccat taaagatccc gcagcgccag ttttcgcgtc acgcgcacca
    67321 aagaccacgc gaccaatgcg actgtggatc atcgctccgg cacacattac acatggttca
    67381 agcgtgacat acaacgtggc gtcgatcaga cgataatttt gcatcaccag accaccctgc
    67441 cgcagggcca tgatttctgc atgtgcggtg ggatcatggc gaccaatcgg gcggttccag
    67501 ccttcgccga ttacccgatt gttatgcact aataccgcgc cgaccggcac ttcccgctca
    67561 tcccaggcac gtttcgccag cgtcagcgcg tgacgcatcc agtattcgtg gctaaattcg
    67621 acttcagaca aaaagaaaac tccggttata aaagcgcggc gcattataca cggacgctat
    67681 gctttactct attccagttg ctggagttca ccgcgcgggg ttactcgcca acgatgctga
    67741 cagaaataaa gcagcggatt gtcctgatta ctgtcgctat agccactgta cagccgcagc
    67801 ggagtgccga ttttgcgctc cagttgtgcg accttttcat gtcccagaca acgcatcgtc
    67861 aatacccaac caccatagcc acgctgaatt tggctggcga taagattaac ccgcggcagc
    67921 cagggcgtat cgaaataaac cgcttcaacc agcggctgcg gagagccggt aatcaaccag
    67981 atatcagcat cggaacttaa caggtaggtg gttaatcgct cctgaaccag cggaaaggcg
    68041 gtaacattgt cgcgaaacca gcgcacgaaa tcggcctgca acgtctgtaa acgtgcttcg
    68101 ctgtgaccaa aagtgcaccc ccacagaagc agactcatcg gccagcgtgc cgcacgacct
    68161 tttatcaata acgcaatggc tataatcggt aacaacggca ggacaagtaa cgcattcagc
    68221 ggttggcgac gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg atgcaatgtt
    68281 ccatctaagt caaaaaacac cacacgacgc tcgtgagttg ccaaaccata ctcctctgga
    68341 ctgaaacatc ctgattaatt ttcgctacta catagcctaa cagatagatc atcacttttc
    68401 cggcaacaat cgaccgcatc atggctaact ggaattttta attcatggca attagcggca
    68461 atggaatata aaattcactc gcgtgtctct catattgcct gttgtcgcca ctatttcatc
    68521 gcgaggaaag atatgaacgg cttacttcgt atccgtcagc gttaccaggg gcttgcccag
    68581 agtgataaaa aactggcgga ttatctgctg ctacaacctg atacggcgcg ccatttaagc
    68641 tctcagcaac tggccaacga agccggagtc agtcagtcca gcgtcgtgaa gttcgcgcaa
    68701 aaactcggct ataaaggttt tccggcgctt aagttggcgt tgagtgaagc gctggcaagc
    68761 cagccggaat cgccctccgt gcccattcat aaccaaatcc gcggtgatga tccgttacgg
    68821 ctggtcggcg aaaaactgat taaagaaaat actgccgcta tgtacgcaac gctaaacgtt
    68881 aatagtgaag agaaactgca tgaatgcgta acaatgttgc gctctgcgcg gcggataatt
    68941 ctgaccggta ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa gctgatgaag
    69001 attggcttca atgctgccgc agtgcgcgat atgcatgcgc tactcgcaac agtacaggcg
    69061 tcgtcccctg acgatctgtt attagccatt tcctacaccg gtgtacgacg cgagttaaac
    69121 ctggcggcag atgagatgct gcgagtgggc ggaaaagtgc tggcgattac cggctttact
    69181 ccgaatgccc tgcaacagcg tgcttctcat tgcctgtata ccattgccga agaacaagcg
    69241 acaaacagcg cttcaatctc tgcttgtcac gctcagggaa tgttaacgga tttgctgttc
    69301 attgcgctga ttcagcagga tctggaactg gcaccagaac gtattcgtca tagtgaagcg
    69361 ctggtgaaaa aactggtctg agtaaagaat gcgcgtataa tgcccgcccg gtttgtgttg
    69421 ttttgagagt ttccttatgg cgttgttaat cactaaaaaa tgcatcaatt gtgatatgtg
    69481 tgaacccgaa tgcccgaatg aggcgatttc aatgggagat catatctacg agattaacag
    69541 cgataagtgt accgaatgcg tagggcacta cgagacacca acctgccaga aggtgtgccc
    69601 gatccccaat actattgtga aagatccggc gcatgtcgag acagaagaac agttgtggga
    69661 taaatttgtg ctgatgcacc acgcggataa aatttaatat tctactctgg aagtagagta
    69721 ttaattatat tactgggaag ccttaacgcc attatattta tttaattgat gacattagca
    69781 taatcattca ctaagttaat ttatatagta tctgccaaga cacttattta tagttattaa
    69841 aggcgcgtcc gattggttca ccggacgcac cttaagtacg tttccttgtg ttataagaac
    69901 agaaggatca gctgtaaaac agcaatgatg attttgatga cccgtttaat caggtatcgg
    69961 caatcagtca ttcgtttttc cttaaacaag gaatgcagcc gttcagatta gcccttacat
    70021 ctccccaaaa ctgaacgtgc gagttattga gggtgcatgc tgcactccac accagagctt
    70081 tgacgacacc actcgtttca atgggggaat tctgtggcat ggtgtaaagc acagcaaaat
    70141 cttcaataac gaagccaatt ttaatgtact tacgaattgg cggtcacgtt aatctttcca
    70201 tcaatattgc tttcttcgta aaggctcgag tttttatgct aaagattgca agttgcttgt
    70261 aaaagataag tacactgatc cataatcgct gttgttgagg gtgcatgctg cacaaaatta
    70321 aagttaaaaa gtaaaacccc cgttccttac cagttcgggg gttttacttt ttaaagagaa
    70381 cggtattatt ttaactttca ataattaccg tggcacaagc atagtggcgt tcatctgcca
    70441 gcgttacatg catatttaca acgccgagct tttccgccag ttttaatgcc tcaccccata
    70501 gccgtagccg tggtttgccg agctcatcat tgaatacttc aaattgatta aacgccagac
    70561 cattgcggat cccggtgcca aacgcttttg ctgcggcttc tttcacagca aaacgcttcg
    70621 ccagaaaacg caccggctgg tggtgcgttt tccagattgc ccattcgtta tcgcttaata
    70681 cgcggcgtgc caggcgctca ccggatcggg cgatcactgc ttctatgcga gcgatctcta
    70741 cgatatccgt gcctaaacct aatattgcca ttagccacgc gcttccagca tcagacgctt
    70801 catttctgcc accgcatctt tcagtccggt catcactgca cgaccaataa tggcatgacc
    70861 gatattcagt tcatgcatct cagggatggc ggcaatagct ttcacgttgt gataggtcag
    70921 accgtgacca gcgttaactt tcagaccgag actagcggca aaggtcgcgg ctttggcgat
    70981 acgcgccagc tcttgcgcct gttcggcgtc agttttggca tcagcatagc aaccggtgtg
    71041 gatctcgata aacggtgcgc caacctctgc cgcagctttg atctgctctt catcggcgtc
    71101 gataaagaga gaaacctgga tcccggcatc tgccagacgt ttgcaggcat cgcgcatttt
    71161 gtcacgctgc cctgcgacgt ccaggccgcc ttcggttgtt acttcctgac gcttttccgg
    71221 taccagacag caaaaatgtg gcttcgtctc aacggcgatc gccagcatct cttcggtcac
    71281 cgccatctcc agattcatgc gggtatccag cgtctgacgc aggatgcgca catcacggtc
    71341 agtaatgtgg cggcgatctt cacgtaaatg cacggtaatg ccgtccgctc ccgcctgctc
    71401 agcaataaac gccgcctgca ccggatccgg gtaagcagta ccgcgtgcgt tacgcagcgt
    71461 tgcaatatgg tcaatgttga cgcctaacag taattcagcc atgacaatcc tcatcattca
    71521 taatgtgttt tcaccgttcg cttaggcata aactgccgga acagttccct gctctttaat
    71581 ggtttaccgc caagatacgg cttaagcgcc atgcgggtaa agcgtttcgc ggcgcgtagt
    71641 gtgtctgcgt caggaaattc ccgcgcgttt aacgctttta actgccttcc ggtgaacgtt
    71701 ttattgtcga taacgacgct tgcgataaac cctttttctt cgcgataacg atacgtcatg
    71761 gtgtcatcta ccggctcgcc gctacccgca caatgggtaa aattgacgcc atagcccaga
    71821 tgcccgagca gtgccagttc aaagcggcgc agcgcgggtt ctggcgtacc agtggcacca
    71881 gcaagagact gaatgcagtg caagtaatcg aaaaagagtt cagagaagcg cgtctcgtat
    71941 tccagtacgc gggagagaag ttcgttgatg tacagaccgc tgtaaagcgt gataccgctt
    72001 aatggcagcg ccagcgagac agcttcagca ctgcgcagcg ttttgacttc gccacgcccg
    72061 ccgaaacgta gcaagagagg ggtgaaaggc tgtaatgcac ctttcagagt agagcgttta
    72121 gagcgtgcgc ctttggcaac cagacgcacg cgacccgatt cctccgtgaa gacgtccagc
    72181 atcaggctgg tttcgctcca cgggcgacta tgcaggacaa atgcgcgctg ccagccttcc
    72241 atcggagtga ctcttaaaga tcgtcaacgt aaccgagact gcgcagtgcg cgttcgtcgt
    72301 cggcccaacc ggatttcact tttacccaca gttcaaggtg gacaggcgct tcgaacattt
    72361 cctgcatgtc tttacgcgct tcaatcccga tggttttgat cttggcccct ttgttgccaa
    72421 taaccatctt cttctgccct tcacgctcaa cgagaatcaa accattgatg tcataaccac
    72481 cgcgttcgtt agagacgaaa cgttcgatct ccacggtcac ggagtacggc agttcagcgc
    72541 cgaggaaacg catcagtttt tcgcggatga tttcagacgc cataaaacgc tgtgagcgat
    72601 cggtgatgta atcttccggg aagtgatggg tcgcttcagg gagatgctta cgcacgattg
    72661 ctgcgatagt gtcgacattc agcccggttt cagcagagat cggcacgata tcgaggaagt
    72721 tcatctgact tgccaggaac tgtaagtgcg gcagcagatc ggctttttcc tgcacgttat
    72781 ccactttgtt caccgcgagg atcaccggcg ctttgccatc gcgcagtttg ttgagcacca
    72841 tttcgtcgtc cggcgtccag cgggtgcctt caacgacaaa aatcaccagc tcaacgtcgc
    72901 caatagagct gctcgccgct ttgttcatca ggcggttaat agcgcgtttt tcttccatat
    72961 gcaggccagg ggtatcaacg tagatcgcct gatacgcgcc ttcagtatgg atccccacaa
    73021 tgcggtgacg agttgtttgc gctttgcggg aggtgatgga gatcttctgc cccagcagtt
    73081 tgttcaacaa tgtggatttg ccaacgttcg gacgtccgac gatggcaata aatccgcagt
    73141 aacttttatc gatgctcatt ccagctccag ttttttcaac gcctgttcgg cggcagcctg
    73201 ctcagcctta cgacggcttg aacctgtgcc aaccaccggt tcactcaggc cgctgacctg
    73261 gcagtggata gtaaattcct gatcgtgcgc ttcgccacgt acctggacta ccagataagt
    73321 cggcagcggc agatggcgac cctgcaaata ttcttgcaag cgcgttttcg gatctttttg
    73381 tttatcgcct gggctaattt cgtccaaacg agtttgatac cagttgagga ttaatttctc
    73441 gacggtttga atatcactgt cgaggaatac accaccaatt aatgcttcga cggtgtcggc
    73501 gagaattgac tcacgacgaa atccaccgct tttaagttca cctggcccta aacgtaagca
    73561 ctcgcctaac tcaaattcgc gcgccagttc cgccagcgta ttgccacgga ccagcgtggc
    73621 gcgcatccgg ctcatatcgc cttcatccac acgagggaaa cggtgataaa gcgcattggc
    73681 gataacgtag ctcagaatag agtcgcctaa aaattctaaa cgctcgttat gtttgctgct
    73741 ggcactacga tgagttaatg cctgctgcaa cagttcctga tgattaaaag tgtagcccag
    73801 cttccgttga agccgattaa ttacgatggg gttcatgcga taccaataaa taaatgcgtc
    73861 aacaattcag cacacgaaac agacctgata tacatggctc tgctaactgc ttcgctgcag
    73921 tttcagttta ccggtatatg gggaccaacg ctgtttcgtg tgccgtggca acctggaggt
    73981 gccaacctta aacttcgggg gaatattcta tacacaacga cgggggatgt cgttagccac
    74041 gggagattta tctcataaat aattcacgtt gtcgccataa cggcgacaac gtgaacgaag
    74101 atgggctatt aatggatgcc gccaatgcga cttaagcgca cacctgtcgg ccattcgcct
    74161 tcttgcttat cgaaactcat ccagatagcc gttgcccgac cgaccagatt cgcttcaggc
    74221 acaaaacccc agtaacggct gtccgcgctg ttgtcgcggt tgtcgcccat catgaagtat
    74281 tgtcccggcg gaacaatcca ggttgccagt tgttgccctg gctgctggta atacatcccc
    74341 acctggtcct gcgcaatcgg cactgtcaga attcggtgcg tcacatcacc cagtgtctct
    74401 ttacgctcgg aaagacgaat tccattttct ttggtttcgt ttttcggcac ttcaaagaat
    74461 ccgctggtcg cttccccacc attacggcgt gagaaggtct gaacgaaatc gctcggttcc
    74521 acgttggagt aggtgaccgg tagcgcgttt tcacacgcct ggccggaact gcatcccggt
    74581 tgaatcgtca gctcttttga gaccggatcg taagtgactt tatcgcccgg taaacccacc
    74641 gcgcgcttga tgtaatcaag ctttggatct tccggatatt taaagaccac gatatcgccg
    74701 cgtttcggat gaccggtttc gatcagcgtt ttctggtaga taggatcttt aatgccataa
    74761 gcaaacttct ctaccagaat aaaatcaccg attaacagag tcggcatcat cgaacctgac
    74821 gggatctgga acggttcata aataaacgaa cgtacaatca atacgatagc cagcaccgga
    74881 aaaacagaag caccggtttc cagccagcca ggcttcggcg caaccttttt cagggttgct
    74941 ttatccagtg agtcaccggc agccgcctgc gccgctgcct gacgttcccg ccgtttaggt
    75001 gcgaaaaaga atttatccac gcaccataaa atgcccgtca ccagtgtggc aatcaccaga
    75061 atcagggcaa acatattcgc catgccaact cctaagggtt atttgttgtc tttgccaacg
    75121 tgcagaatgg cgaggaacgc ttcctgcggc agctcgacgt taccgatctg cttcatgcgt
    75181 ttcttacctt ctttctgctt ctgcagcagc tttttcttac ggctgatatc gccgccataa
    75241 catttagcca gtacgttttt acgtagctgt ttcacggtgg atcgcgcaat aatgtgcgta
    75301 ccaatcgctg cctgaatggc gatatcaaac tgctggcgtg ggatcagatc tttcatcttc
    75361 tccaccaact cgcgaccgcg gttttgcgaa ttatcacggt gggtgatcag cgccaacgca
    75421 tcaacacgtt caccgttgat taatacgtct acacgtacca tgtcggacgc ctggaagcgt
    75481 ttgaagttgt aatccagaga cgcataacca cgcgaggtag atttcaggcg atcgaagaag
    75541 tcgagcacca cttccgccat cgggatctcg tacgtcagcg ccacctgatt accgtggtaa
    75601 accatattgg tctgcacgcc gcgtttttct acgcacagcg taataacgtt gccgagatat
    75661 gcctgcggca gcagcatgtg acattcggca atcggctcgc gcagttcgta gatgttattt
    75721 accgctggca gcttggatgg actgtcgacg taaataactt ctcttgacgt ggtttcaact
    75781 tcatacacta cggtcggcgc agtggtgatc agatccagat cgtattcacg ttccagacgt
    75841 tcctggatga tctccatgtg cagcaggccg aggaagccgc agcggaaacc aaagcccagc
    75901 gcgctggagc tttccggctc atagaacagt gaagcatcgt tcaggctgag tttacccagc
    75961 gcgtcacgga aggcttcata gtcgtcggaa cttaccggga acagaccggc gtatacctgc
    76021 ggtttgactt tcttaaagcc aggcagcgcc ttttctgccg gattacgcgc cagcgttaag
    76081 gtatcgccga ctggagcgcc gtggatatct ttaatcgcac ataccagcca gcctacttcg
    76141 ccacatttca gttcagtgcg gtcaacctgt ttcggcgtga agatgcccag acggtcggcg
    76201 ttataggtct gcccggtact catgactttc actttgtcgc ccttacgcag ggtgccgttt
    76261 ttaatacgga taagtgaaac aacgcccagg tagttgtcga accatgagtc gataattagt
    76321 gcctgcaacg ggccttccgg atcgccttcc ggcggcggaa tgtcgcgcac cagacgttcg
    76381 agaacgtcct gcacgccaac gccggttttc gctgaacagc gcaccgcgtc ggtggcgtcg
    76441 atgccgacga tatcttcaat ttcttccgcc acgcgttcag gatcggctgc cggcaggtca
    76501 atcttgttca gtaccggcac cacttcgaga tccatttcca tggcggtgta gcagtttgcc
    76561 agggtttgcg cttctacgcc ctgcccggcg tcgaccacca gcaatgcacc ttcacaggca
    76621 gccagcgaac gggaaacttc ataggagaag tcaacgtggc ccggggtgtc gataaagttc
    76681 agctgatagg tttcgccgtc agacgctttg tagtccagcg tcacgctttg cgctttgatg
    76741 gttatgccac gctcacgctc aagatccatg gaatcgagaa cctgcgcctc catttcacgg
    76801 tcagacaggc caccgcagat ctggataata cggtcagaca gcgtcgattt accgtggtca
    76861 atgtgagcta tgatcgaaaa gttacgtata ttcttcataa agtatgatta ttgtgcctta
    76921 atgcccggtt aaccaggctt ttagaagtcg ctgttctgag cttaacgtct gtattaatag
    76981 aaacgccgca ttctacacta caacattgag gcgaggaaat gttcataccg tatggattgt
    77041 ggtatctgga aacgtcctcg catttgttat gcaaaatgca acaaagccag tgaaatcact
    77101 ggctcgcgtc ttccgaagat gtttcaaatc gcacaaggcc aggcggcaat gccacgctta
    77161 agatgatcgg ttgccattct gcccgggcag caaacttacg cgagtagccg cgcgcaatca
    77221 ggaacccgcc aataccgccg aggaccgcac cacataatgc tgcgacgtca gaagcaaaga
    77281 gtagctgaaa tagcgaagcg atgaggaata atcccaccag cggcgacata taaaccagta
    77341 atgcggagct aagcaggctg ccttcggcga tccccaattc cactttttgc cccggcacta
    77401 acggctcatc acagggtacg acaatggtat gcgtggtttg cgggccaagt ttatttaaca
    77461 cgcggctacc gcaaccggcg cgtgaagcgc agctgctgca tgaaacttta acatcacaac
    77521 tgaccagcgc ctgcccgttt tgccaggaga cgacggtagc ccactctttg atcattgcgc
    77581 tgccccgaac ttaatattct cggcaatgcg tttcgccgtt tgcggcggca gttcaccgac
    77641 aatggtgatt tcggcgttat cacgtacgct tgtactgacg gttctgcgtc cggtgcgcaa
    77701 catctgatcg gtgctcgatg gcgtagcgcg gttaacgttt accgagaagc tgaataatcc
    77761 gtcggaatag agacgtgatt cgataggcat gttgtccatc gtcggtagcg gacgtcgact
    77821 actggaaact tcgctaaaac cctgtggcaa ccaggttggc gtccagctga atttagcttt
    77881 ttcacctaca ggaacagaaa gcaacggcgg caaatttgcc ttcgccagcg tctgcatact
    77941 gctactgata tcctgattga cgttaaaagc aatcacgcga aattgttcca gcgtttcacc
    78001 atcgcgatca aggagatcaa ccctcatcgg taatttcgat tcgatgtcca tccacacgat
    78061 gtagctgtag cgtgtaccat ctcgggcaac cacgcgaatg acttcgcaaa gacgatcagc
    78121 aatacgcgtg cgcccgacgg agataaagtc gtagtaagga gaaaggcgtt tgaaatcggt
    78181 atagatgagc gatggcagag aatcaacaat gtaatcgcca ttaagcgtga acggttcaag
    78241 tcccggttca aaatagctga tttcattgcc acgctgtacc acttcccggc gcgggccatc
    78301 catttgcaac aattgtgcaa gaggacggtt atcgaggcgt gcatgtcgat aacgcagaga
    78361 ctcaacaccc tgtttattga tgctgatgaa tgacagctcg taattcagtg actgactggc
    78421 caggttcatc tgctgtaata acgccccgga cgcgggagtg gccgaggcgt tagcagagaa
    78481 taacaggcta cctgtcacta atgacatggc aaaccaaagt tgcttcatta ctgcgattgc
    78541 gttcctaaag tttgaattcc tggcacctgt acagcggctt gctgggtttg cgcctgctca
    78601 aactgaagct gttcagagtg gagtcggcgt tgcagttcgt aatcctgcaa cattgcatta
    78661 atgcgacgac gctgctcctg tacctgctgc tgttgaccat tgtttgcggt cgcttcagaa
    78721 ggtactccca ggcttaccgg gctggcttta cccatcatcg gcagtgtatt aaataccggc
    78781 gtttcgggct gctgggacgt ttcagattgt ccattatagt gctggacgcc aacgataact
    78841 gcaagcgata cgcatgctgc tacgcccatt tgggtaagct gtgccgccca cggacgtact
    78901 ttctgccaga atggcatttt ctgccattga tgcggcgcag gctgggcttc cgggatcaat
    78961 gtcgccggtt gacgtactgg ctcttcttca atggcggcca tcacgcgtga agagatatcg
    79021 aaatggagca cctcgggagt atcaccccgc attgagtcac ggattaagtg atagctttcc
    79081 caggttttct gcatttctgg gttatgagcc agttcgttaa gcagctcact atccagcgtt
    79141 tcgccatcca ttaaagcgga aagttgttct ttctgcatgc ctaataccct tatccagtat
    79201 cccgctatcg tcaacgcctg ataagcggtt gaactttgtt atcaatagct tccctcgctc
    79261 ggaagatacg tgaacgcacc gtacctaccg gacaatccat gatagcggct atctcttcat
    79321 agctcaggcc atccagctcc cgcaaggtta ttgccatgcg caaatcttcc gggagggact
    79381 caatagttcg gaaaactatc tgtctcagtt cttctgacaa cattaagttc tcagggttcg
    79441 aaatttcttt caacgcgccg ccactttcga agttttcagc ttcaatggca tccacatcac
    79501 tggaaggtgg acgacgcccc tgagcaacca ggtaattttt cgctgtattt acagcaatcc
    79561 gatacagcca tgtataaaaa gcgctatctc cccggaacga atccagcgca cgataggctt
    79621 taataaaagc ttcttgtacc acatcgggaa catcacccga cggcacatag cgggaaacca
    79681 gactcgccac tttatgctga tagcgcacta ccagtaggtt aaaggctttc tgatctccct
    79741 tctggacccg ttcaaccagg acctggtccg ttaactgctc gctcatccga ggtaaagtct
    79801 ccccaaacca aatttccacg cgctatcgaa acgccactcc attagctgca atttgagcaa
    79861 gcaaagggtt agagtgtctc gtttttgtaa agttccgtaa cgcatctgtt tttgtttgtc
    79921 atgctgtaga cggatcatta tctatcatta taagtctaca gaatctgaac atcgcattat
    79981 ctgtgtagaa atgcccattt aactgcctga agagtaaccc aacggccttt ttatttcacc
    80041 acctaatcct ccaccagcca gtaacttctc tttttctcgc cgccctgcgt cagcgtgttt
    80101 agccactgta acaaatatta aaatagcagg tgtttattcg cacaacatga tgctatgctg
    80161 accaaacggt gtttagtaaa ttaaacaaag aaaatgaata ctctcccaga acattcatgt
    80221 gacgtgttga ttatcggtag cggcgcagcc ggactttcac tggcgctacg cctggctgac
    80281 cagcatcagg tcatcgttct aagtaaaggc ccggtaacgg aaggttcaac attttatgcc
    80341 cagggcggta ttgccgccgt gtttgatgaa actgacagca ttgactcgca tgtggaagac
    80401 acattgattg ccggggctgg tatttgcgat cgccatgcag ttgaatttgt cgccagcaat
    80461 gcacgatcct gtgtgcaatg gttaattgac caaggggttt tgtttgatac ccacgttcaa
    80521 ccgaatggcg aagaaagcta ccatctgacc cgtgaaggtg gacatagtca ccgtcgtatt
    80581 cttcatgccg ccgacgccac cggtagagaa gtacaatcca cgctggtgag caaggcgcag
    80641 aaccatccga atattcgtgt gctggagcgc agcaacgcag ttgatctgat tgtttctgac
    80701 aaaattggcc tcccgggcac gcgacgggtt gttggcgcgt gggtatggaa ccgaaataaa
    80761 gaaacggtgg aaacctgcca cgcaaaagca gtggtactgg caaccggcgg tgcgtcgaag
    80821 gtttatcagt acaccaccaa tccggatatt tcttctggcg atggcattgc tatggcgtgg
    80881 cgcgcaggct gtcgggttgc caatctcgaa tttaatcagt tccaccctac cgcactgtat
    80941 cacccacagg cacgcaattt cctgttaacg gaagcactgc gcggcgaagg cgcttatctc
    81001 aagcgcccgg atggcacgcg ttttatgccc gattttgatg agcgcggcga actggccccg
    81061 cgcgatattg tcgcccgcgc cattgaccat gaaatgaaac gcctcggcgc agattgtatg
    81121 ttcctcgaca tcagccataa gcccgccgat tttattcgcc agcatttccc gatgatttat
    81181 gaaaaattgc tcgggctggg gattgatctc acacaagaac cggtaccgat tgtgcctgct
    81241 gcacattata cctgcggtgg tgtaatggtt gatgatcatg ggcgtacgga cgtcgagggc
    81301 ttgtatgcca ttggcgaggt gagttatacc ggcttacacg gcgctaaccg catggcctcg
    81361 aattcattgc tggagtgtct ggtctatggc tggtcggcgg cagaagatat caccagacgt
    81421 atgcctgatg cccacggcgt cagtacgtta ccgccgtggg atgaaagccg cgttgagaac
    81481 cctgacgaac gggtagtaat tcagcataac tggcacgagc tacggctgtt tatgtgggat
    81541 tacgttggca ttgtgcgcac aacgaagcgc ctggaacgcg ccctgcggcg gataaccatg
    81601 ctccaacaag aaatagacga atattacgcc catttccgcg tctcaaataa tttgctggag
    81661 ctgcgtaatc tggtacaggt tgccgagttg attgttcgct gcgcgatgat gcgcaaagaa
    81721 agccgggggc tgcattttac gctggattac ccggaactgc tcacgcattc cggtccatcg
    81781 atcctttccc ccggcaatca ttacataaac agataaaaag cctgggtcag cgccgtatac
    81841 gcttcggaat agttctggtc tggcccacga atgactaagc gatcgctaaa gcattctccc
    81901 gcctgcgggg agaatgccag cagcacccga tgcggcagtc gcgcttcgtt ttccgccaca
    81961 tccgtccgca aacgtaaatg ccagcccatg cttaatgcca gttcagtaaa accattacca
    82021 atctgctctg gcagcaccac gcagaaaaat ccctcttcgg taatgcactc cgccgcacag
    82081 gtcagcagcg atgggtgatc aagcgtagtg gtatagcgag cctgttcccg ttgaggtgtc
    82141 gcgcactcta ctccctgctg atagtaaggt gggttactga tgattaaatc gaagcgtact
    82201 gtctgctgtg tgatccactg ctgaatatcc gccgtatgga cgttaatccg ctctgcccac
    82261 ggggactggt tgatattttc ctgcgcctgc gcggcagctt cactttccag ctcaacggca
    82321 tcaattatta cgctgtcact ggttcgctgc gccagcatca gtgccagcaa cccgctaccc
    82381 gcgccgatat caaggcaacg tttcacccca gccaccggtg cccatgcgcc cagcaaaatg
    82441 ccatccgttc ccactttcat cgcacagcga tcgtgagcaa caaaaaactg tttaaaagta
    82501 aatccattac gacgaagcac ggatgtagac tgtgacatga aaataaaacc ttgcaggaaa
    82561 aacggcgata gcaccgggtg agaacaatac ccgagaagcg atatccatac aaacagatga
    82621 agattgcagc cgtaacgtct ataatcagcg ccccacacag aggtagaaca tgactgtaac
    82681 gactttttcc gaacttgaac tcgacgaaag cctgctggaa gccctccagg ataaaggttt
    82741 cactcgcccg accgccattc aggctgccgc cattccgcct gcgctcgatg gccgtgatgt
    82801 actcggttct gcgccgacag gcaccggtaa aacggcggcg tatctgctgc cagcgttgca
    82861 gcacctgctc gatttcccgc gtaagaaatc cggtccgccg cgtattttga tcctcacccc
    82921 aactcgcgag ctggcgatgc aggtggccga tcatgcccgc gaactggcga aacatacgca
    82981 tctggatatc gccaccatca ccggcggcgt agcctatatg aaccacgcgg aagtgttcag
    83041 cgaaaatcag gacatcgtgg tcgccacgac cggacgtctg ctgcaataca taaaagaaga
    83101 gaacttcgat tgccgcgcgg ttgaaacgct gatcctcgac gaagcagacc gtatgctgga
    83161 tatgggcttc gctcaggata tcgaacatat tgctggcgaa acgcgctggc gtaaacagac
    83221 cctgctcttt tcggcaacgc tggaaggcga tgcgattcag gactttgccg agcgtctgct
    83281 ggaagatccg gtggaggttt ctgccaatcc gtccacccgt gagcgcaaaa aaattcatca
    83341 gtggtattac cgcgccgatg acctggaaca taaaaccgca ttgctggtgc atctgttaaa
    83401 acagccggaa gcgacccgct caattgtgtt tgtgcgtaag cgtgaacgtg tgcatgagct
    83461 ggcaaactgg ctgcgcgaag cgggcatcaa caactgctat ctcgaaggtg agatggtaca
    83521 aggcaagcgt aacgaagcga tcaagcgttt gaccgaaggt cgcgtaaacg tactggttgc
    83581 aaccgatgtt gccgcgcgcg gtatcgacat tcctgacgtc agccacgtct ttaacttcga
    83641 tatgccgcgc agtggcgata cttatttgca ccgtatcgga cgtaccgcgc gcgccggtcg
    83701 taaaggcacc gcaatttcgc tggtggaagc ccatgaccat ttgctgctgg gcaaggttgg
    83761 ccgttacatt gaagagccga ttaaagctcg cgttattgat gagttacgcc cgaaaacgcg
    83821 tgcgccaagc gagaagcaga ccggcaagcc atcgaagaaa gtactggcaa aacgcgccga
    83881 gaagaaaaaa gctaaagaga aagagaaacc gcgtgtgaaa aaacgccatc gcgacaccaa
    83941 aaatattggt aagcgccgta aaccaagcgg aacgggcgtg ccaccgcaaa cgacagaaga
    84001 gtaatctcaa tgccgggttt aagcctggta ttaaaagtgc tggcaaacgc aaaactgcct
    84061 gatgcgctac gcttatcagg cctacgtggc tcatgcaata tattgaattt gcacgatctt
    84121 gtaggccgga taaggcgttc acgccgcatc cggcatgaat aaagcgcact ttgtcgacaa
    84181 gttaaatgcc gggtttaatc ccgacattat ttttatccag ccacaaaact ctcttcaaca
    84241 cactgaataa aagtgtgcat cgccgggcta accgcttttc cagcatggtg tgcgcacatg
    84301 gccgtaatgg tctgcgactg ttcgccaaaa ggcaattcaa ttaattctcc gctctcaagc
    84361 tctttttcca ccgcaaagcg gggcaagtaa ctgacaccga tattcgccgc gacacaacgt
    84421 ttgatacttt caatgcttaa cagctcaatg gtgttttcca ccgtgatccg ccgctgacgc
    84481 agcgtgctct caaatatctg ccggaagaca cattgcggtt cgttgataat aaagctactg
    84541 gcgttatgtc ttcccggctc agtaaaatcg acatctgcaa tttgcggtga agccaccagc
    84601 accagcgatt gttcacccaa ctctcgtcgg ttcagggcat catcattccc tacgcgataa
    84661 aagacgccga catcggcctc atcattcagt agcgcatcac ggatcacgta acagttcagc
    84721 gactgcaacg ataaacgcac ttttggtgcg cgttgccgaa aacgctgcaa cacctgcggc
    84781 attcggtaag agagcaacgt ttcgcccgaa acaacgcgta gttccccgtc cggatctgac
    84841 tcctttttgg cagcctcacg aagcgtatcc atcactcggg taagttcata aatgtgcggc
    84901 agcaactttt ttccttcgcg ggtaaggcac atccgtcggc caattttctc aaataactgg
    84961 actgaaaact cctgctcaag ctgctgaata tgaaaagtca ccgtcgattg tgtacagcac
    85021 aatttttgcg aagctcgcaa aaaggaaccc tcttccacca cggttttaag cgtaataaaa
    85081 cggcgcagat ccataacccc aaacctatcg aaaatatcga atctagaata taaaaacatt
    85141 cattttttta aatgttccgt gtcgggtact gtctaccaaa acagaggaga taacaagtga
    85201 caccgaccct tttaagtgct ttttggactt acaccctgat taccgctatg acgccaggac
    85261 cgaacaatat tctcgccctt agctctgcta cgtcgcatgg atttcgtcaa agtacccgcg
    85321 tgctggcagg gatgagtctg ggatttttga ttgtgatgtt actgtgtgcg ggcatttcat
    85381 tttcactggc agtgattgac ccggcagcgg tacacctttt gagttgggcg ggggcggcat
    85441 atattgtctg gctggcgtgg aaaatcgcca ccagcccaac aaaggaagac ggacttcagg
    85501 caaaaccaat cagcttttgg gccagctttg ctttgcagtt tgtgaacgtc aaaatcattt
    85561 tgtacggtgt tacggcactg tcgacgtttg ttctgccgca aacacaggcg ctaagctggg
    85621 tagttggcgt cagcgttttg ctggcgatga ttgggacgtt tggcaatgtg tgctgggcgc
    85681 tggcggggca tctgtttcag cgattgtttc gccagtatgg tcgccagtta aatatcgtgc
    85741 ttgccctgtt gctggtctat tgcgcggtac gcattttcta ttaacgaaaa aaagcggaag
    85801 aggtcgccct cttccgctta gtaacttgct acttaagcct tacaggcttt cagtaaaggt
    85861 acgagcgata acgtcgcgct gctgttccgg agtcagagag ttaaagcgaa ctgcataacc
    85921 ggatacacgg atggtcagct gcggatattt ttccggatgc ttaactgcat cttccagagt
    85981 ttcacgacgc agaacgttaa cgttcaggtg ttgaccacct tcaacgcgaa cttctggttt
    86041 cacttctact ggaacttcac ggtattcaat gtcacccagt ttgcttactg caaccacttc
    86101 atcttctgca taacctgctt ttgcaacgat gcaacgtgct tcgccttttt cgctgtccag
    86161 cagccagaaa gagttcagca gatcgtcgtt agcggcttta gtaatctgga tacctgtaat
    86221 catgtgatgc ctccccggca aaattatttg atttgttcag cctgtcgcgg ccaattggta
    86281 aaaccattgt tgcttgagtg tatatatact cctcaaccac ccttgattct ttgatttaaa
    86341 tcaataaaaa ccacacatcg attgtggtcg taaatggatt ttattgtttt acatcaactt
    86401 atgcgggtgt gaaattttac caatttacat ttttttgcac tcgtttaagt ctaaaaaatg
    86461 agcatgattt tgttctgtag aaagaagcag ttaagctagg cggattgaag attcgcagga
    86521 gagcgagatg gctaacgaat taacctggca tgacgtgctg gctgaagaga agcaacaacc
    86581 ttattttctt aatacccttc agaccgtcgc cagcgagcgg cagtccggcg tcactatcta
    86641 tccaccacaa aaagatgtct ttaacgcgtt ccgctttacc gagttgggtg acgttaaagt
    86701 ggtgattctc ggccaggatc cttatcacgg accgggacag gcgcatggtc tggcattttc
    86761 cgttcgtccc ggcattgcca ttcctccgtc attattgaat atgtataaag agctggaaaa
    86821 tactattccg ggcttcaccc gccctaatca tggctatctc gaaagctggg cgcgtcaggg
    86881 cgttctgctg ctcaatactg tgttgacggt tcgcgcaggt caggcgcatt cccacgccag
    86941 cctcggttgg gagacgttta ctgataaagt catcagcctg attaaccagc accgcgaagg
    87001 cgtggtgttt ttgttgtggg gatcacatgc gcaaaagaaa ggggcgatta tagataagca
    87061 acgccatcat gtactgaaag caccgcatcc gtcgccgctt tcggcgcatc gtggattctt
    87121 tggctgcaac cattttgtgc tggcaaatca gtggctggaa caacgtggcg agacgccgat
    87181 tgactggatg ccagtattac cggcagagag tgagtaaatt tgcggggaaa tgccggatgg
    87241 cagagttgcc acccggctga tttatcaggc tttattctga cgccaccatt caccaagcaa
    87301 aacgccggtt gcgacagaga tattcagccc ggcaacgttg cccgtaccgt caatcttcac
    87361 gcgcagatcg ttcggatcgc gtgcggcatc cggtaaccct tcatattcct gacccagcac
    87421 cagtaccatt ttcgctggca gactagtttt gaacagcggt ttaccctgct cgctggaagt
    87481 ggtcactacg gtgtaacctg cctgacggaa atcatccagc acgttaacaa tgttgtcgcc
    87541 agtaatcggc tgaacgtgct ctgcgccgcc ttctgcggta cggatagccg cccccgactc
    87601 cagcagtgcc gcatcctgca ccacgacacc tttcacgccg aagtgcgcgc agctgcgcat
    87661 catgccaccc aggttatgcg ggttagattc gttttccagt gccagaacgc aatcctgcgc
    87721 gcctgcctgg cttacccact gctgcacggt tgtaccgtta cgctttttga tcaagaagca
    87781 aacgccgccg tgatgttccg tgcctgacgc ttttgtcaat tccgcttcat ccaccacatg
    87841 gtacgctttg cggtttgctg ccatccagcg caaggcttct ttaaaacgcg gcgttacact
    87901 ctggataaac caggcgcgaa caatcgcttc cggacggctc tggaaaagtg cctgacatgc
    87961 attttcgccg tagacgcggg tttcttccgc acgctgacga cgcaacactt ccggatcaat
    88021 aaaactttta ccactgatgc caccgtgatc ggccttttcc ggcgtctcat cacccggcgc
    88081 gcgggaaacc gtgcgccacg gcgaatcttc ccacttgcgg tcacggggct gactctgttt
    88141 gtcatcgcgg gcggggcgac ggccaccgtc agcacgagat tttcctggac gcccgccccc
    88201 tttcccggta cgcgggttgt gggtacgttt atcagaatca tcatcactgc ggacatacat
    88261 cactttgacc ttgccgcttt tacctttcat ttcatcgttc atgcttttct ccaccagcgc
    88321 tgcgcgaagc gcgcagatta cccgaagtcc gcgcggttcg ccatgatttc gtaccaaagc
    88381 ctgcgactat catacctatt gaataaaaca gattgttgtc tggaacaatg tccccgataa
    88441 tatgtaacat attagaaaca taccggcgtc gttgccgata agtctcctta ctcatcccga
    88501 ggttagttat gaataccgtt tgtacccatt gtcaggccat caatcgcatt cccgacgatc
    88561 ggatcgaaga tgcggcaaaa tgcggacgct gcggtcacga cttgtttgac ggagaggtga
    88621 ttaatgcgac cggtgaaaca ctcgacaaat tgctgaagga tgatctacct gtggtgatcg
    88681 acttctgggc accgtggtgc ggcccctgcc gtaatttcgc accaattttt gaagatgtcg
    88741 cgcaagagcg tagcggtaaa gtgcgctttg tgaaagtgaa taccgaagct gaacgtgaat
    88801 tgagcagtcg ctttggaatt cgtagtatac cgacgatcat gattttcaaa aacggtcagg
    88861 ttgtcgacat gcttaatggc gcagtaccga aagcgccgtt cgatagctgg ctgaacgaat
    88921 ctctttaatc ttaccggggc gcatcttgtg ccccgttttc tcctctgcga caatggcgtt
    88981 ttttcgacgc tctcttatga ccgaaaacgg tgttctccag ttacgcgccg agcgtattgc
    89041 gcgcgcaaca cgtccttttc ttgcccgcgg taatcgcgtt cgtcgctgcc aacgctgtct
    89101 tttgccagag aaattatgtc tctgttcgac aattacccca gcacaagcaa aaagtcgctt
    89161 ctgtttgctg atgttcgaca ccgagccaat gaagcccagt aataccgggc gtctgattgc
    89221 tgatattttg cctgataccg ttgcgtttca atggtcgcgt accgaaccct cgcaggattt
    89281 gctggagttg gtgcaaaacc cggactatca gccaatggtg gtctttcccg cttcgtatgc
    89341 tgatgagcaa cgggaagtga tcttcacacc acctgccggt aagccaccgc tgtttatcat
    89401 gctcgatggt acctggccgg aagctcgcaa gatgtttcgt aaaagtccgt atctggataa
    89461 tcttcccgtc atttccgtcg atctttcccg gctttctgcc tatcgcctgc gtgaagccca
    89521 ggctgcaggc caatattgta ctgccgaggt agccatcgca ctgttagata tggccagcga
    89581 taccggggcg gcagcagggt taggcgagca ttttacccgc tttaaaacac gctatctggc
    89641 aggaaaaacg caacatctgg gtagcatcac agcagaacag ttagaaagcg tttaaaatca
    89701 ttcggtcact tctgcgggag accggtatga gtcagcgagg actggaagca ctactgcgac
    89761 caaaatcgat agcggtaatt ggcgcgtcga tgaaacccaa tcgcgcaggt tacctgatga
    89821 tgcgtaacct gctggcggga ggctttaacg gaccggtact cccggtgacg ccagcctgga
    89881 aagcggtgtt gggtgtgttg gcctggccgg atattgccag cttgcccttt acacccgacc
    89941 ttgcggtttt atgtaccaat gccagccgta atcttgctct tctggaagag ctcggcgaga
    90001 aaggctgtaa aacctgcatt attctttccg ccccggcatc gcaacacgaa gatctccgcg
    90061 cctgcgccct gcgccataac atgcgcctgc ttggaccaaa cagtctgggt ttactggctc
    90121 cctggcaagg tctgaatgcc agcttttcgc ctgtgccgat taaacgcggc aagctggcgt
    90181 ttatttcgca atcggctgcc gtctccaaca ccatcctcga ctgggcgcaa cagcgtaaga
    90241 tgggcttttc ctactttatt gcgctcggcg acagcctgga tatcgacgtt gatgaattgc
    90301 ttgactatct ggcacgcgac agtaaaacca gcgccatcct gctctatctc gaacagttaa
    90361 gcgacgcgcg acgctttgtt tcggcggccc gtagtgcctc tcgcaacaaa ccgattctgg
    90421 tgattaaaag cggacgtagc ccggcggctc aacgtctgtt gaacaccacg gcaggaatgg
    90481 atccggcgtg ggatgcggct attcagcgtg ccggtttgtt gcgggtacag gacacccacg
    90541 aactgttttc ggcggtggaa acccttagcc atatgcgccc gctgcgtggc gaccggttga
    90601 tgattatcag caacggtgct gcgcctgccg cgctggcgct tgatgcctta tggtcacgca
    90661 atggcaagct ggcaacacta agcgaagaaa cctgccagaa actgcgcgat gcactgccag
    90721 gacatgtggc aatctctaac ccgctcgatc tgcgcgatga cgccagcagt gagcactata
    90781 ttaaaacgct ggacattctg ctccacagcc aggattttga tgcgctgatg gttattcatt
    90841 cgcccagcgc cgctgctccc gcaacagaaa gcgcgcaagt attaattgaa gcggtaaagc
    90901 atcatccccg cagcaagtat gtttctctgc tgacgaactg gtgtggcgaa cattcctcgc
    90961 aagaggcacg acgattattc agcgaagccg ggctgccgac ctaccgtacc ccggaaggaa
    91021 ccatcactgc ttttatgcat atggtggagt accggcgtaa tcagaagcaa ctacgcgaaa
    91081 cgccggcgtt gcccagcaat ctgacctcca ataccgcaga agcgcatctt ctgttgcaac
    91141 aggcgattgc cgaaggagca acgtcactcg atacccatga agttcagcct atcctgcaag
    91201 catatggcat gaacacgctc cccacctgga ttgccagcga tagcaccgaa gcggtgcata
    91261 ttgccgaaca gattggttat ccggtggcgc tgaaattgcg ttcgccggat attccacata
    91321 aatcggaagt tcagggcgtc atgctctacc tgcgtacagc caatgaagtc cagcaagcgg
    91381 caaacgccat tttcgatcgc gtaaaaatgg cctggccgca ggcacgggtc cacggcctgt
    91441 tggtgcaaag tatggctaac cgtgctggtg ctcaggagtt gcgggttgtg gttgagcacg
    91501 atccggtttt cgggccgttg atcatgctgg gcgaaggcgg tgtggagtgg cgtcctgaag
    91561 atcaagccgt cgtcgcgtta ccgccgctga acatgaacct ggcccgctat ctggttattc
    91621 aggggatcaa aagtaaaaag attcgtgcac gcagtgcgct acgcccattg gatgttgcag
    91681 gcttgagcca gcttctggtg caggtttcca acttgattgt cgattgcccg gaaattcagc
    91741 gtctggatat tcatcctttg ctggcttctg gcagtgaatt taccgcgctg gatgtcacgc
    91801 tggatatctc gccgtttgaa ggcgataacg agagtcggct ggcagtgcgc ccttatccgc
    91861 atcagctgga agaatgggta gaattgaaaa acggtgaacg ctgcttgttc cgcccgattt
    91921 tgccagaaga tgagccacaa cttcagcaat tcatttcgcg agtcaccaaa gaagatcttt
    91981 attaccgcta ctttagcgag atcaacgaat ttacccatga agatttagcc aacatgacac
    92041 agatcgacta cgatcgggaa atggcgtttg tagcggtacg acgtattgat caaacggaag
    92101 agatcctcgg cgtcacgcgt gcgatttccg atcctgataa catcgatgcc gaatttgctg
    92161 tgctggttcg ctcggatctc aaagggttag gcttaggtcg acgcttaatg gaaaagttga
    92221 ttacctatac gcgagatcac ggactacaac gtctgaatgg tattacgatg ccaaacaatc
    92281 gtggcatggt ggcgctagcc cgcaagctcg ggtttaacgt tgatatccag ctcgaagagg
    92341 ggatcgttgg gcttacgcta aatcttgccc aacgcgagga atcatgagta aggtactgga
    92401 aatgttgacc acttaatcgg gactggtgtt attattgccc gcttatgtcg tctgcattgc
    92461 acagaggacc cttcaatgaa cagagaagaa atgcactgtg atgttgtcaa aatttaagcg
    92521 taataaacat caacaacacc ttgcccaact acccaagatt tctcaatcag ttgatgatgt
    92581 cgatttcttt tacgctcccg ccgacttccg ggagacgctg ctggaaaaaa tagccagcgc
    92641 gaagcagcgc atttgcattg tcgccctgta tctcgaacag gatgacggtg gcaaaggcat
    92701 tctgaacgcg ttgtatgaag ctaaaaggca gcgtccggaa ctggatgtgc gggtgctggt
    92761 cgactggcat cgtgcacaac gtggacgcat tggcgctgcg gcatctaaca ctaacgctga
    92821 ctggtactgc cgcatggcgc aggaaaatcc gggcgtagat gttccggttt atggcgttcc
    92881 aatcaatacc cgtgaagccc ttggtgttct gcactttaaa ggctttatca tcgacgatag
    92941 cgtactttat agcggtgcca gcctgaacga tgtttacctg catcagcacg ataaatatcg
    93001 ctacgaccgt tatcatctta tccgtaaccg taagatgtca gacattatgt ttgaatgggt
    93061 tacacagaat attatgaatg gccgcggcgt taatcgtctg gatgatgtta atcggccaaa
    93121 aagcccggaa atcaagaacg atattcgtct gttccgccag gagctgcgtg atgccgctta
    93181 tcatttccag ggcgatgccg acaacgatca gctttccgta acgccgctag tggggctggg
    93241 gaaatcgagt ctgttgaaca agaccatttt ccatcttatg ccttgtgccg agcagaaact
    93301 aaccatctgt acgccatact tcaacctgcc agcaatcctt gtgcgcaata ttatccagtt
    93361 gctgcgcgaa gggaaaaagg tcgaaattat tgttggtgat aaaaccgcga acgacttcta
    93421 cattccggaa gatgaacctt tcaagataat tggcgcattg ccttatctct atgagatcaa
    93481 tctgcgtcgt ttcctgagcc gtttgcagta ttacgtcaat actgaccagc tagtggttcg
    93541 gttatggaaa gatgacgaca acacctatca cctaaaaggg atgtgggttg atgataagtg
    93601 gatgttgatc accggtaata acctgaaccc gcgcgcctgg cgtctggatc tggaaaacgc
    93661 cattttgatc cacgatccac aactggagct tgcgccgcag cgagagaaag aactggagct
    93721 gatccgcgag cataccacca tcgttaagca ctatcgcgat ctgcaaagta ttgccgatta
    93781 tccggtgaag gttcgtaaac tcatccgccg tttgcgccgt atccgcatcg accgattaat
    93841 tagccgcatc ctgtaatcac aaccccgtcc tgtacggggt ttgttttttg gaggccacgt
    93901 tttgcgtatt ctttttgtct gttcgctatt gttactttct ggatgcagcc atatggctaa
    93961 cgatagctgg agcgggcagg ataaagctca acactttatc gcctcggcga tgctttccgc
    94021 cgccggaaat gaatattcac agcatcaggg gatgagccgg gatcgcagtg ccatgtttgg
    94081 attgatgttc tctgtcagtt tgggggcgtc aaaagagctt tgggatagcc gccccgaagg
    94141 gagcggctgg agctggaagg atttggcctg ggatgtcgcc ggtgcaagca ccggctatac
    94201 cgtctggcaa ctgacccgtc actaaagacg catccccttc cctttgcgat gtagcatcaa
    94261 agaaaccaga aacgccacca cggccatcaa ggtcacatac cagaagaagg ctgtttccat
    94321 tcctattgat ttcagcgaca acgctacgta ctccgccgaa ccaccaaata tagcattagc
    94381 gaccgcatat gacaggccaa cgcctaatgc gcgaacctgt gccgggaaca tctcagcctt
    94441 cagtattcca ctgattgatg tataaaaact cactatcagc agggcacaca tcaccagacc
    94501 aaaagcggca taaggcgagg aaacgttttg caatgctgag agaataggaa cggtaaaaat
    94561 ggctgccagc gaaccgaaac ataacattga ggtacggcga ccaatcttat ccgacagcgc
    94621 gccaatgagt ggttgaataa gcatgaatac aaacaatgcg gcagtcataa tgccactcgc
    94681 cacgttggca tgcattcccg cagtatttac cagatacttc tgcatataag tggtgaaggt
    94741 atagaaacaa agggagcccg cagcggtaaa accgagaacc atgatgaatg cacggcgatt
    94801 gcgccataat cctttcagag atccagcttc ttttaaagcg cgcgtttctt gttgcgaagt
    94861 ttcatctaat tgacgacgta accacaacgc cacaacagct aacacagctc ctaacgcgaa
    94921 aggaatacgc catccccact ctctgagtgc agcgtcttcc atggtgtgtt gtaaaaccac
    94981 gacaaccagt agggctagca gttgtccgcc gatcaacgtc acatactgaa atgatgcgta
    95041 aaaacctttg cgcccttcaa cggcaacttc actcatatag gtggcgctgg tgccatattc
    95101 tccgccaacg gataatccct gaaataaacg agcgagaagc aataatgccg gagcccacgt
    95161 acctatagtt tcataacctg ggaggcaggc gataaccagc gatccgaaac acatcataca
    95221 caccgataac agcatcgatt ttttgcgacc atgtttatcg gctatgcggc caaatagcca
    95281 accgcctatt gggcgcatca ggaatcccgc agcaaaaaca cctgctgttt gtagtagttg
    95341 agtcgtcgtg ttcccggaag ggaagaaaat gtgggcgaag tagagtgaac agaacgagta
    95401 gacatagaaa tcgaaccact cgaccagatt acctgaagag gcccccacaa tcgcccaaat
    95461 gcggcgacgt gtatcactac ttgtcagttt gctgtctgcc gttacagtac tttcagccat
    95521 gccattatgt ctcctgccgt aatccgatgc ttttgtcggt cgcttttgtt tatttctttt
    95581 ggtaaagaaa atgttataca tttgttgcgt atcattatgc aaccttaacc atgaatttag
    95641 ttagcaggaa aatggttatt gaggagctta aggataaatt tctggtgagg agaacacgta
    95701 tggaggtggg caagttgggg aagccgtatt cgctgctgaa tctggcatat gtgggagtat
    95761 aagacgcgca gcgtcgcatc aggcattttt ttcaccaacg caaaaaggcc atcctgacgg
    95821 atggcctttc gcttaatttg atgcctggca gttccctact ctcgcatggg gagaccccac
    95881 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
    95941 cgcgctacgg ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat
    96001 ctgtatcagg ctgaaaatc
//
LOCUS       DAZFJF010000004        93881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFJF010000004 DAZFJF010000000
VERSION     DAZFJF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 93881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.004"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(583..900)
                     /gene="yehK"
                     /locus_tag="ACHKAU_000290"
     CDS             complement(583..900)
                     /gene="yehK"
                     /locus_tag="ACHKAU_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO5529427.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            complement(962..4594)
                     /gene="yehI"
                     /locus_tag="ACHKAU_000291"
     CDS             complement(962..4594)
                     /gene="yehI"
                     /locus_tag="ACHKAU_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO5529428.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            complement(4604..8398)
                     /locus_tag="ACHKAU_000292"
                     /pseudo
     CDS             complement(4604..8398)
                     /locus_tag="ACHKAU_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            complement(8539..10572)
                     /gene="metG"
                     /locus_tag="ACHKAU_000293"
     CDS             complement(8539..10572)
                     /gene="metG"
                     /locus_tag="ACHKAU_000293"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO5529429.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            10704..11813
                     /gene="apbC"
                     /locus_tag="ACHKAU_000294"
     CDS             10704..11813
                     /gene="apbC"
                     /locus_tag="ACHKAU_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO5529430.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            12076..12357
                     /gene="yehE"
                     /locus_tag="ACHKAU_000295"
     CDS             12076..12357
                     /gene="yehE"
                     /locus_tag="ACHKAU_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO5529431.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            12650..13192
                     /gene="yehD"
                     /locus_tag="ACHKAU_000296"
     CDS             12650..13192
                     /gene="yehD"
                     /locus_tag="ACHKAU_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5529432.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKEIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            13273..13947
                     /gene="yehC"
                     /locus_tag="ACHKAU_000297"
     CDS             13273..13947
                     /gene="yehC"
                     /locus_tag="ACHKAU_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5529433.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            13963..16443
                     /gene="yehB"
                     /locus_tag="ACHKAU_000298"
     CDS             13963..16443
                     /gene="yehB"
                     /locus_tag="ACHKAU_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5529434.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            16457..17491
                     /gene="yehA"
                     /locus_tag="ACHKAU_000299"
     CDS             16457..17491
                     /gene="yehA"
                     /locus_tag="ACHKAU_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO5529435.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(17573..17911)
                     /gene="rcnB"
                     /locus_tag="ACHKAU_000300"
     CDS             complement(17573..17911)
                     /gene="rcnB"
                     /locus_tag="ACHKAU_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO5529436.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(18130..18954)
                     /gene="rcnA"
                     /locus_tag="ACHKAU_000301"
     CDS             complement(18130..18954)
                     /gene="rcnA"
                     /locus_tag="ACHKAU_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO5529437.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            19075..19347
                     /gene="rcnR"
                     /locus_tag="ACHKAU_000302"
     CDS             19075..19347
                     /gene="rcnR"
                     /locus_tag="ACHKAU_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO5529438.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      19439..19535
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            19570..20358
                     /gene="thiM"
                     /locus_tag="ACHKAU_000303"
     CDS             19570..20358
                     /gene="thiM"
                     /locus_tag="ACHKAU_000303"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO5529439.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            20355..21155
                     /gene="thiD"
                     /locus_tag="ACHKAU_000304"
     CDS             20355..21155
                     /gene="thiD"
                     /locus_tag="ACHKAU_000304"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO5529440.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            21220..22038
                     /gene="yegX"
                     /locus_tag="ACHKAU_000305"
     CDS             21220..22038
                     /gene="yegX"
                     /locus_tag="ACHKAU_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO5529441.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            22090..22836
                     /gene="yegW"
                     /locus_tag="ACHKAU_000306"
     CDS             22090..22836
                     /gene="yegW"
                     /locus_tag="ACHKAU_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5529442.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(22810..23775)
                     /gene="yegV"
                     /locus_tag="ACHKAU_000307"
     CDS             complement(22810..23775)
                     /gene="yegV"
                     /locus_tag="ACHKAU_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5529443.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(23772..24776)
                     /gene="yegU"
                     /locus_tag="ACHKAU_000308"
     CDS             complement(23772..24776)
                     /gene="yegU"
                     /locus_tag="ACHKAU_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO5529444.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(24773..26050)
                     /gene="yegT"
                     /locus_tag="ACHKAU_000309"
     CDS             complement(24773..26050)
                     /gene="yegT"
                     /locus_tag="ACHKAU_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO5529445.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            26307..27359
                     /gene="fbaB"
                     /locus_tag="ACHKAU_000310"
     CDS             26307..27359
                     /gene="fbaB"
                     /locus_tag="ACHKAU_000310"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO5529446.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            27669..28523
                     /gene="gatY"
                     /locus_tag="ACHKAU_000311"
     CDS             27669..28523
                     /gene="gatY"
                     /locus_tag="ACHKAU_000311"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO5529447.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            28552..29814
                     /gene="gatZ"
                     /locus_tag="ACHKAU_000312"
     CDS             28552..29814
                     /gene="gatZ"
                     /locus_tag="ACHKAU_000312"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO5529448.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            29824..30276
                     /gene="gatA"
                     /locus_tag="ACHKAU_000313"
     CDS             29824..30276
                     /gene="gatA"
                     /locus_tag="ACHKAU_000313"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO5529449.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            30307..30591
                     /gene="gatB"
                     /locus_tag="ACHKAU_000314"
     CDS             30307..30591
                     /gene="gatB"
                     /locus_tag="ACHKAU_000314"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO5529450.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            30595..31950
                     /locus_tag="ACHKAU_000315"
     CDS             30595..31950
                     /locus_tag="ACHKAU_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO5529451.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            31998..33038
                     /gene="gatD"
                     /locus_tag="ACHKAU_000316"
                     /pseudo
     CDS             31998..33038
                     /gene="gatD"
                     /locus_tag="ACHKAU_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            33144..33917
                     /locus_tag="ACHKAU_000317"
     CDS             33144..33917
                     /locus_tag="ACHKAU_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5529452.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(33999..34898)
                     /gene="yegS"
                     /locus_tag="ACHKAU_000318"
     CDS             complement(33999..34898)
                     /gene="yegS"
                     /locus_tag="ACHKAU_000318"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO5529453.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            35313..35630
                     /gene="yegR"
                     /locus_tag="ACHKAU_000319"
     CDS             35313..35630
                     /gene="yegR"
                     /locus_tag="ACHKAU_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO5529454.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(35960..37321)
                     /gene="yegQ"
                     /locus_tag="ACHKAU_000320"
     CDS             complement(35960..37321)
                     /gene="yegQ"
                     /locus_tag="ACHKAU_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO5529455.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(37424..37720)
                     /locus_tag="ACHKAU_000321"
     CDS             complement(37424..37720)
                     /locus_tag="ACHKAU_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5529456.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            complement(37722..38018)
                     /locus_tag="ACHKAU_000322"
     CDS             complement(37722..38018)
                     /locus_tag="ACHKAU_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5529457.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            complement(38227..38559)
                     /gene="yegP"
                     /locus_tag="ACHKAU_000323"
     CDS             complement(38227..38559)
                     /gene="yegP"
                     /locus_tag="ACHKAU_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO5529458.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(38750..39472)
                     /gene="baeR"
                     /locus_tag="ACHKAU_000324"
     CDS             complement(38750..39472)
                     /gene="baeR"
                     /locus_tag="ACHKAU_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO5529459.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(39469..40872)
                     /gene="baeS"
                     /locus_tag="ACHKAU_000325"
     CDS             complement(39469..40872)
                     /gene="baeS"
                     /locus_tag="ACHKAU_000325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO5529460.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(40869..42284)
                     /gene="mdtD"
                     /locus_tag="ACHKAU_000326"
     CDS             complement(40869..42284)
                     /gene="mdtD"
                     /locus_tag="ACHKAU_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5529461.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(42285..45362)
                     /gene="mdtC"
                     /locus_tag="ACHKAU_000327"
     CDS             complement(42285..45362)
                     /gene="mdtC"
                     /locus_tag="ACHKAU_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO5529462.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(45363..48485)
                     /gene="mdtB"
                     /locus_tag="ACHKAU_000328"
     CDS             complement(45363..48485)
                     /gene="mdtB"
                     /locus_tag="ACHKAU_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO5529463.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(48485..49732)
                     /gene="mdtA"
                     /locus_tag="ACHKAU_000329"
     CDS             complement(48485..49732)
                     /gene="mdtA"
                     /locus_tag="ACHKAU_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO5529464.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            50011..50067
                     /locus_tag="ACHKAU_000330"
     CDS             50011..50067
                     /locus_tag="ACHKAU_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5529465.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            50339..50395
                     /locus_tag="ACHKAU_000331"
     CDS             50339..50395
                     /locus_tag="ACHKAU_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5529466.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            50668..50727
                     /gene="ibsA"
                     /locus_tag="ACHKAU_000332"
     CDS             50668..50727
                     /gene="ibsA"
                     /locus_tag="ACHKAU_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO5529467.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            50948..51607
                     /gene="yegL"
                     /locus_tag="ACHKAU_000333"
     CDS             50948..51607
                     /gene="yegL"
                     /locus_tag="ACHKAU_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5529468.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            51604..52365
                     /gene="pphC"
                     /locus_tag="ACHKAU_000334"
     CDS             51604..52365
                     /gene="pphC"
                     /locus_tag="ACHKAU_000334"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO5529469.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            52362..54281
                     /gene="yegI"
                     /locus_tag="ACHKAU_000335"
                     /pseudo
     CDS             52362..54281
                     /gene="yegI"
                     /locus_tag="ACHKAU_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(54314..55666)
                     /gene="yegD"
                     /locus_tag="ACHKAU_000336"
     CDS             complement(54314..55666)
                     /gene="yegD"
                     /locus_tag="ACHKAU_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5529470.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            55800..56657
                     /gene="alkA"
                     /locus_tag="ACHKAU_000337"
     CDS             55800..56657
                     /gene="alkA"
                     /locus_tag="ACHKAU_000337"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO5529471.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(56696..60006)
                     /gene="dgcE"
                     /locus_tag="ACHKAU_000338"
                     /pseudo
     CDS             complement(56696..60006)
                     /gene="dgcE"
                     /locus_tag="ACHKAU_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            60324..60965
                     /gene="udk"
                     /locus_tag="ACHKAU_000339"
     CDS             60324..60965
                     /gene="udk"
                     /locus_tag="ACHKAU_000339"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO5529472.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            61057..61638
                     /gene="dcd"
                     /locus_tag="ACHKAU_000340"
     CDS             61057..61638
                     /gene="dcd"
                     /locus_tag="ACHKAU_000340"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO5529473.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            61660..63513
                     /gene="asmA"
                     /locus_tag="ACHKAU_000341"
     CDS             61660..63513
                     /gene="asmA"
                     /locus_tag="ACHKAU_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO5529474.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(63787..65370)
                     /gene="yegH"
                     /locus_tag="ACHKAU_000342"
     CDS             complement(63787..65370)
                     /gene="yegH"
                     /locus_tag="ACHKAU_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5529475.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            66029..67168
                     /gene="wza"
                     /locus_tag="ACHKAU_000343"
     CDS             66029..67168
                     /gene="wza"
                     /locus_tag="ACHKAU_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO5529476.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            67174..67617
                     /gene="wzb"
                     /locus_tag="ACHKAU_000344"
     CDS             67174..67617
                     /gene="wzb"
                     /locus_tag="ACHKAU_000344"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO5529477.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            67620..69782
                     /gene="wzc"
                     /locus_tag="ACHKAU_000345"
     CDS             67620..69782
                     /gene="wzc"
                     /locus_tag="ACHKAU_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO5529478.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            69875..70714
                     /gene="wcaA"
                     /locus_tag="ACHKAU_000346"
     CDS             69875..70714
                     /gene="wcaA"
                     /locus_tag="ACHKAU_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO5529479.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            70717..71205
                     /gene="wcaB"
                     /locus_tag="ACHKAU_000347"
     CDS             70717..71205
                     /gene="wcaB"
                     /locus_tag="ACHKAU_000347"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO5529480.1"
                     /translation="MLEDLRTNSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            71202..72419
                     /gene="wcaC"
                     /locus_tag="ACHKAU_000348"
     CDS             71202..72419
                     /gene="wcaC"
                     /locus_tag="ACHKAU_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO5529481.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            72394..73611
                     /gene="wcaD"
                     /locus_tag="ACHKAU_000349"
     CDS             72394..73611
                     /gene="wcaD"
                     /locus_tag="ACHKAU_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO5529482.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            73622..74368
                     /gene="wcaE"
                     /locus_tag="ACHKAU_000350"
     CDS             73622..74368
                     /gene="wcaE"
                     /locus_tag="ACHKAU_000350"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO5529483.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            74384..74932
                     /gene="wcaF"
                     /locus_tag="ACHKAU_000351"
     CDS             74384..74932
                     /gene="wcaF"
                     /locus_tag="ACHKAU_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO5529484.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            74958..76079
                     /gene="gmd"
                     /locus_tag="ACHKAU_000352"
     CDS             74958..76079
                     /gene="gmd"
                     /locus_tag="ACHKAU_000352"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO5529485.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            76082..77047
                     /gene="fcl"
                     /locus_tag="ACHKAU_000353"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             76082..77047
                     /gene="fcl"
                     /locus_tag="ACHKAU_000353"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO5529486.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            77050..77529
                     /gene="gmm"
                     /locus_tag="ACHKAU_000354"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             77050..77529
                     /gene="gmm"
                     /locus_tag="ACHKAU_000354"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO5529487.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            77526..78749
                     /gene="wcaI"
                     /locus_tag="ACHKAU_000355"
     CDS             77526..78749
                     /gene="wcaI"
                     /locus_tag="ACHKAU_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO5529488.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            78752..80188
                     /gene="cpsB"
                     /locus_tag="ACHKAU_000356"
     CDS             78752..80188
                     /gene="cpsB"
                     /locus_tag="ACHKAU_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO5529489.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            80381..81751
                     /gene="cpsG"
                     /locus_tag="ACHKAU_000357"
                     /gene_synonym="manB"
     CDS             80381..81751
                     /gene="cpsG"
                     /locus_tag="ACHKAU_000357"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO5529490.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            81887..83281
                     /gene="wcaJ"
                     /locus_tag="ACHKAU_000358"
     CDS             81887..83281
                     /gene="wcaJ"
                     /locus_tag="ACHKAU_000358"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO5529491.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            83283..84761
                     /gene="wzxC"
                     /locus_tag="ACHKAU_000359"
     CDS             83283..84761
                     /gene="wzxC"
                     /locus_tag="ACHKAU_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO5529492.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            84907..86187
                     /gene="wcaK"
                     /locus_tag="ACHKAU_000360"
     CDS             84907..86187
                     /gene="wcaK"
                     /locus_tag="ACHKAU_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO5529493.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            86184..87404
                     /gene="wcaL"
                     /locus_tag="ACHKAU_000361"
     CDS             86184..87404
                     /gene="wcaL"
                     /locus_tag="ACHKAU_000361"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO5529494.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            87415..88809
                     /gene="wcaM"
                     /locus_tag="ACHKAU_000362"
     CDS             87415..88809
                     /gene="wcaM"
                     /locus_tag="ACHKAU_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO5529495.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            88984..89877
                     /gene="galF"
                     /locus_tag="ACHKAU_000363"
     CDS             88984..89877
                     /gene="galF"
                     /locus_tag="ACHKAU_000363"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO5529496.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            90249..91334
                     /gene="rfbB"
                     /locus_tag="ACHKAU_000364"
     CDS             90249..91334
                     /gene="rfbB"
                     /locus_tag="ACHKAU_000364"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5529497.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            91334..92233
                     /gene="rfbD"
                     /locus_tag="ACHKAU_000365"
     CDS             91334..92233
                     /gene="rfbD"
                     /locus_tag="ACHKAU_000365"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO5529498.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            92291..93169
                     /gene="rfbA"
                     /locus_tag="ACHKAU_000366"
     CDS             92291..93169
                     /gene="rfbA"
                     /locus_tag="ACHKAU_000366"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5529499.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            93174..93716
                     /gene="rfbC"
                     /locus_tag="ACHKAU_000367"
     CDS             93174..93716
                     /gene="rfbC"
                     /locus_tag="ACHKAU_000367"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO5529500.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNK
                     PIAISEKDKKGSSILRLNDY"
BASE COUNT        23350 a        23108 c        24558 g        22865 t
ORIGIN      
        1 cgggatgtga atatttatgt tgggctattg gcaaaaaata tctggaaagt agatcgcaga
       61 aaataaaaaa acctgctggt gaggcaggtt cttttattta ctacagtgac gctaacttgc
      121 tgattgtgca acatttcgta ccaacgaata aattataacc atatccacac tccattgcaa
      181 ttcagtgagt gagatttata atcctgcccg ttgggaggtt gtgccggatc ggaccaattt
      241 taagtcctgg ctgccggacg ggtggtgccg caggcggtgc cctgatccgg agagcaacgt
      301 ttcgtaccaa catacgtaaa ccagtatcaa caggcagtgt aagggtaagg tgctggtgtt
      361 gcgctcctta ataaggagag cggatggaca cgctgacaca aaagttaacc gtgctcattg
      421 ccgtactgga gttattagtg gctctgttac ggttgattga tttgttgaag taacgggcaa
      481 attgaaacgc atctaagggg gaataccgtt tcccccttta agaaagctat actcgtcata
      541 cttcaagttg catgtgctgc gactttaatt atctagagta tattattcta tttcttggtt
      601 gaatatattc tgtagttcac gattaaaaat tgctattaca gtttcaggtg attcacctcg
      661 taatttccgc tcatcaatac actcatctct tatataaatc aacgcatcat ttattagcgg
      721 gtcagtatct tctcgatcac gttgatgaaa tgttggacgt agccggaatc gctccagtcg
      781 atagacggaa cctgtaaata attcagagca tttatggatc tcaagtctga aggaaaacgg
      841 attttcaggt acagggatct cataatcgta aatagcaacc agctcttttt gcacgatcat
      901 cgctttcccc gctggtaagt cgtgttgata acgggcagtt gcatcactgc ccgctgtgtt
      961 tttaatggaa aatgacgtca ggtgcgctta acgcctcact caaatcaaac acatcaagtt
     1021 cggcaaaagt gacggattta ttgtcacggt gcactcgaag agaacggaag ctcattagcg
     1081 ctttgctatc aacattgtag tggttaaaac cgggtgaaaa cttaaggacc agccggtact
     1141 cacccaggtc atagacaaat gcgtcctcat aacctgactc ccagcctttg tatcgcattc
     1201 ccataagctg cccaacggta gcggatttac cttcccagcg agtcaggctg ttactggttg
     1261 actcgtcagg tgtgagcagc accgtgcggc gcgacaactg gcgaaaaggc ggcataattt
     1321 cgtaatcggc aaaaagctgt gcaaattcac tgcgcatttc tactgtcatt tctaacgggt
     1381 gggcaatgcc aatcagagcg tccgcaggca ggtcaattgg ctcatcttgc gcattgcaga
     1441 actccccctc tgcggccaca cgaaaggctt tgagtaaaca acgcggttca tttgccggat
     1501 aaacccccca tattaatcgc tgggtaacca gacgggtaaa gggatgatta acaaacagag
     1561 aatgaaaatc tgccagcgac cagcgtcggg tggtacgtag ggcagcttca agacgcggga
     1621 gcaggttttt gctcacctga gtagcatctt tttttagccc ttttagtcgg gccagtgcct
     1681 cgggcgcttt cagttgatcg tcatcggcgc gcaaccgggg aacgcttttc tggcgaacgt
     1741 tttgctgatt aaagataacc gggttgaggt tttcatcgaa gcgaacggta aactgccggg
     1801 gaccaaaatc aaaactcaac gcctgcggat catcaaggcc cagggttggg actaatctgt
     1861 ccgctagctc ttcctggctt agcccgcgat tttctgcgac tacctgaaga aattccgccg
     1921 cgttatcacg taaggggcgg gatttagccc gttgcgatat atgatgcagc tgtatcagcg
     1981 ccatatcatt attcatcagg gtaaggatgt tcaggccgct gacagcacgg gcagatttgc
     2041 cttcttgtgg ccaggcgagg atctgtgtcg tcagatcccg tgccgtgctt tcgtcaccaa
     2101 agagacttaa cgccagaaat gcccagttgt cttttgcggg ggcaccagct tgttgccacg
     2161 cagtgaagag atcccaggca aaagctgcca gcgtttgtgg ctggcagaac gttttcagtt
     2221 gttccagccc gctataaaaa cgtcccccct gggtaaagcg cagcatttcg ccgataattt
     2281 ccagagcgtc accggtaaca ggctgattat tagtgataag tcgaggcctg gaccacattg
     2341 ccggatgcca gaaatcaggg gcttttggaa tgcgtgccgg gtaaatgtcc attggaccct
     2401 gtttaagcaa ctgctccagg gcagaccata catctgtacg ctgccagcgg tttgccacgg
     2461 tttgtagcaa ttcgccatgt ccctgttcgt acagtaaacg cagggcaagt aatgcggctt
     2521 cgctattatc gctgggtttg gtaaagacaa gagggataag tgcactagcc gtatgttccg
     2581 gccattgtaa aatccactgg cgagccagat cacgctgcgc cgcgaaacgt cgccagacgc
     2641 gggcaacagg cagcgccagt tctgttgcgc caaaatttaa aattaacgga aatgtttctt
     2701 ttggacgatg tgaaaaagcc aacagcagac ctggtagcgc atccaatccg aggatactga
     2761 gaaaatagtc ctcacccgcg aacagatgct ttttctcaat gatacgcagc cagaaggtca
     2821 aagccatctc acgcggcaac tgtgccaata aatacaggtt ccagacactc ttttccggac
     2881 gataacgtga gaagttgtca ttttcccata tgttaattaa cgcttctatg tgaccgctgc
     2941 ggagcgcctg ttctgctaat tgtgcttgcg gggagttcca gccattcagg atctcttcaa
     3001 cccgcccctg gtagtaatgc cactgaacaa tgagtgaatt gcgtgcagca tccagttttc
     3061 cggggagtat ataattcaga aacagccata catcatcccc aaaacccagt tcctgtaata
     3121 caactcgtcc acttttttgt tccatgcgtt ccgcaagaga ttccgtgctg aaataacggg
     3181 cagactcagt ggcggcaagc ctctcttgtt cgcctggttg ccagtaaatt tgcggagtta
     3241 attcgagcgg tgccaaatca agacgtggag ctgtcatttt tttcttacta cgccacggtg
     3301 gtgaaacgag catttcaggc agactgctgt tatcggtgct atcaataacc ggttttggcg
     3361 gtgccgataa ataactattc agcactgccc tggcttgagt acttaaccac ggcatcactt
     3421 ctgctgccag ttgtggctgc gatgccatca gcgtttgtaa cagcgtacgc cagtattcat
     3481 cgcgtgcttt ttgctgtgca ggcagctttt tgtcttcgat gattggataa cctgggcggg
     3541 ctggtggttc ttttaacgcc agcagttctg ccagtgcggc gagcgtcgcg tgggggaagt
     3601 ttttactgta tttagccaca cgttgcaggc tgggtttgtt tttatcagcc accagtagca
     3661 aggtgcggat gacttgcgga tggttaattt gtaccagcag actgccgcaa tcttctttat
     3721 gggcatacat tgccaggcgc ggaacggctg ccagtccttg ttcgcgcaaa agggaggcgc
     3781 aggccgcgta gccgaagcgg ttttcttgtg acatgtagct ggcttcgcga tcgctaaata
     3841 cgtccaggcc ccagtatcgt tcgagttttt ttaccgccgt attatcagta gcaacgacct
     3901 ttaaccactc ctttgaatgg agacttgacc gagagctttc aagactggcg agttcattgg
     3961 ctatttcagg tttttccggg aggatcagcg caataaaagg acggcgaatt ttgggtattc
     4021 ctggcagtgc ggcaatgagt ttatcggcac aacgctgcca gcaactttcc tctgccagcg
     4081 acagatgctt acgcaaacgg agatcgaaat cgttataggt cgcggagcca taaccgtagc
     4141 catagtcctc gtcctgatac tgcaaagtga cgaccagtga gtcactctca taacgtcgcg
     4201 caatgcactg acgcgctatc actacatccg tcgcatattc cagcccctct tgttgcacga
     4261 tttcatcaag taactgctgg ctgtcatttt gcgcgataca gatcagagcc gccatggtaa
     4321 gggctgggat tgacggttta tgctcgccag tcaaattgat tgcctgctgg atggcggact
     4381 gccactccag tgagcattta tcataattcc aggtgatatt ttccttctgg cctgcttgtt
     4441 gtagtaattc atctcccaat gtctggatga gacttccagt gataacggac actggttcgc
     4501 ccggccagcg acgatgactg agcggcgttt tgcctttttt atatttcagt tccagttggg
     4561 cgttatccgc cagccacggt aattccttgt ccatgctctt tccttattca aacagggctt
     4621 caatatcgtt aattagctcg ctggcggtga tggcatcgag tacggagaac tgcgcttcct
     4681 gtgttgaatt actcccccag ccatagcttt ctgctttgcc ttcccatagc cacagcttgc
     4741 ttaagagttg ctcggcgcta agttcggctg gcgacatacc aactgcaaag ccttcatcga
     4801 tttccatgat taacgaccag cggcccagag gtttgatcat ccagccgatc cagccaccat
     4861 cctgcggttc accacgcacc caacctttat tcgccagtcc catgacccga ccgctcgggc
     4921 attttctgcc tgcccagcgg gtcaattcgc tggcattgcg ctcggtttct gtcagggcgt
     4981 aactgttacg gtcgagctgg cggaacggcg gtaacagttc atagtcggca aaaagctgcc
     5041 caaaggcggc ggcatccgtc ggtgatatct ccagaacgtg aggaatgccg atagagatat
     5101 cgccttccgg cagggtgaaa agatcatcgt cagcggtgct gtagctgtta tcttctgcta
     5161 cgcgaaagca agccagtagc tggttttcgg cgctataaac gccccaaatc agacggcggg
     5221 ttaagtggcg aaccagcgga tgctcaacca ggaagagctg gaagttttcc ggcgaccagc
     5281 ggcggcgcag gcacatggcg gattccagcc ttgctacctg ctgggcggcg acggtacgcg
     5341 catctttttt cagcaatttg tagcggttaa ccgcatcgtt cgaccgcgat tcatcatcgc
     5401 ttttgttcgg tttgggcagg tctttcaggc ggctgccgga aacatcacgc acaaacggtt
     5461 ttaaggtttc atcaaagctg acggtgaact gccgtgggcc aaaatccagc agcagcgaac
     5521 cgttatcatc cagaccgaga tccggtgcta accgatcttc aagctccgcc accgtgagtt
     5581 cgcggctctc ggcaatgtcg gcaatttttt cttttgcccg ctcctgtaat gctttgaatt
     5641 tcagtttctg ggcgatgccg ttaagctgca taagggcgat atcactaccg atagcagcga
     5701 gaatatccaa cccaacggtg gcgcgtttat gctgggattc accaggccag gcgcgtatta
     5761 atggcgtcag tttgcgggcg gtgtcatcgt taccgagaac gcctaacgca gtgaacgccc
     5821 aactctcttt cgacggcgcg ccagcggtct gccaggcggt aaacagatcc caggcaaatc
     5881 ccgccagtga gtcggcggaa cagacgtctt tcacctgcaa taatcccgga tacagagcct
     5941 cttcctgagg gaagcggagc atttcaccga ggtggaggag ggcgctatct ggcagtgatt
     6001 gggcatttgc ttttaatacc gggcgcgtcc agagcgatgg ctgataaaag gccggaagag
     6061 tggggatttt tgtcgggtga ttatctaagg gatcgagcgc aagcagagcg ttcaccgcat
     6121 cggttacttc cggctggtta taacgtcggg cgatttcttg cagtaatggc tgatgaccgt
     6181 tttcggtaag catacgcaag gcagcgcggg cgttatcctg tgcttcaccg gctttgccga
     6241 gcgccgcagg cagcaggccg gtaagggcat gttccgggta tttcaacagc cagctacggg
     6301 cgttttcgcg tagcgttttc agtttgttga aggcacgggc gacggcagga gccagctcac
     6361 tcgctgcgaa gtaattcgtg atgggcaagg cttcttgtgg atagcgtgca agtgagttta
     6421 ctaacccagg taaccctttg agtccaaaat gtaacatggc gtattcggta tcggtatgtg
     6481 gagatagggt aagggcattc catgctttta ctgctttctc cagcggcaat gttgtcaaaa
     6541 tccccaactg ccaataatgc ccggtaggtg aatgaaacac cttaaattca cttagtaagg
     6601 ttgaataatc ttcatttagc catgcctcaa ctaccgcttc gggagcttta atatatgtac
     6661 cagcgatccg tcgttgaaaa cccatccttg ccaacaggtt ggtagtactt tcttcatggc
     6721 taaccgtgat gtgttttgaa taccagatat atttcgccaa aagttgatta ctgatttctt
     6781 ctgtcagata acagattggc tcaatgccta atggcgctaa atccagcacc ggaatcggcg
     6841 attttttctt tttcgaaagc cagggagggg agactactac ggctggcagt agatcggcgc
     6901 tggcatggtt tgagggctgt gtcagttgtt gctggcatga tttcagtacg gcaactgcgg
     6961 gtgtcgagag ccagggaatg acctgttctg ccagtgctgg ttgtgagata agcattgtca
     7021 ttagcataat gcgccaactg ttctcttctt tttgcccaag aagttccgtc agtgccgcca
     7081 ttgctgcatg tgggaacgca gcaatggctt tcgtcatccg atcgtgacag cgtttagtat
     7141 gcccggctac acgtataagc agtgttaatg cgaacggatg attgatatga cgcaacacat
     7201 cggcgcagta gtcacttgcg gcatagggag caaatcgggg cagggctgca acaccttgct
     7261 cctgtaatac ggtggctgac catatcgcac cacaatagta atcatcaaaa atggcatatg
     7321 gctcgtgata tttttctaat gaggcaagaa tgtgctcatc agtcgccact atttttaacc
     7381 actcaagcga gggtaatttt ttttgcccca gtagacgctg ggcaatttca tgtgcaattt
     7441 ctggtttttc aggtaataac aaagcaatta atggttgccg tcattctgga atatgtggaa
     7501 ttgcggcaat aagtttttgt gcacattttt gccagagaga ctcttccgcc agtgataaat
     7561 gtttacgtag gcgtaattca aatctggaat attgctccaa gtagtcaggt gcaatgccag
     7621 acggcagaat aataatgaca ttattcgcat aatcccattt aatatcaatc tgttgaaggt
     7681 gtattattac atcagtagca tattccagac cgccttcctg gacgatctga tccataacat
     7741 cctcacgcac tttatatctt gtgcatttca tttcaagtgc cgtgagtaca gccattactg
     7801 gtgatggtag ctgtgttgtt ttcaggccgt cgatctgact gatcgcctgg gcgacggtgt
     7861 tttgccattc taaagaacag gcgctgaagt caaatgtggc tgttttatta tcccgttgcg
     7921 acacactaca ggctaagcga cgtaattggg tcaatttgtc tgcttgctga acaggaactc
     7981 cgggaaagcg gcgatgactt aacgtggtgt gtacaacttc gggcggaaaa acgacagcaa
     8041 tatctttcgc cagccacggt aaaacagcat ctgtattttt gtcaggcgca cgggtttctg
     8101 ctacgaaaga gggttgttcc tgaataatgg tggataagtc cgctaccttg agagaacccg
     8161 ttaccgtttg ggaaggttgc aaagaattat ccttcgcttg ctccacatac cccttcttca
     8221 ccttctccgc aatcagctta agctccgctt ttgccgctgc cgcagcatct gaaaaacttt
     8281 ttatctggct ttgccctttg gtgccaactt ttccccaact gatatgcaac tcgtttccct
     8341 gttgctcaac tgcccagaat ttatgtgatt tttcgtcctg atagataaag tgtttcattc
     8401 tgcatccctg taattttatc cagttgaatg ctgatgctac cagtatttat gcgggttaga
     8461 gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc gctgtcgatg
     8521 cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg catccgggct
     8581 tagcaggaaa atatctttcc cgccaggacc agcagccatt accatgcctt cggagatacc
     8641 aaagcgcatt ttacgcggtg ccaggttggc caccataatg gtgtgacgcc cgatcagtgc
     8701 ctgcggatcc gggtaggcag aacgaatacc ggagaagaca ttgcgttttt caccgccgag
     8761 atccagtgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt tttcgatcag
     8821 cgccacgcgc aggtcaactt ttgcgaagtc gtcaaaggtg atggtttcct gaatcggatc
     8881 atctgccagt gggccagtaa ctggcgcggc agctgctttc acttcttctt tagacgcttc
     8941 caccagtgct tcaacctgct tcatatcgat gcggttatac agcgctttga acggattcac
     9001 tttgtggccc agcagcggtt gctggatacc atcccaggtc agttctgtat tgaggaatgc
     9061 ttctgcgcgc tcggtcagtt tcggcagtac cggcttcagg taagtcatca gcacgcggaa
     9121 caggtttatg cccattgagc aaattgcctg caggtcggca tcgcggcctt cctgttttgc
     9181 caccacccac ggagcctgct cgtcgacata gcggttagcc agatcagcca gcgccatgat
     9241 ttcgcgcact gctttaccaa attcgcggct ttcccacgct tcaccaatca cttcagcggc
     9301 atcagtgaag gttttgtaca actgcgggtc agccagttcg cttgccagca cgccgtcaaa
     9361 acgcttgttg ataaagcccg cattacgaga ggccaggtta accactttgt tcacgatatc
     9421 ggcattcaca cgctgaacga aatcctccag gttgagatcg atatcatcaa tgcgcgaaga
     9481 gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca gccaggtgct
     9541 ggctttaata aaggtgccgc gagacttgga catctttgcg ccgttcaccg tcacatagcc
     9601 atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg caggccagaa
     9661 caggctgtgg aagtaaacaa tatctttacc gatgaagtgg tacagctcgg cggtggagtc
     9721 tttcttccag tattcatcga agcttacgct gtcgccgcgc ttgtcgcaca gattcttgaa
     9781 agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc ccggcgcgtt
     9841 cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt gcaggccgga
     9901 ttcaaaccac tcctgcattt tatttgccac ctgctcctgc aacgcaccgc tgcgggtcca
     9961 tgcctgcaac atttcgctga aagagggcag atcaaagaag aagtgttcag aatcacgcat
    10021 taccggcgta gcgccagaaa ccaccgattt cggctcgatc aattcagtcg ggctgtaggt
    10081 cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt tcgggcaggt
    10141 gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca gctgagagat
    10201 ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt ctgacaactg
    10261 gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa agcctgcgaa
    10321 atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga taccgagctg
    10381 ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga agttgacctc
    10441 gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct ccagcatatg
    10501 gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa ttttcttcgc
    10561 gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat agtaccaaat
    10621 gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag ctgatggttt
    10681 atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca ccagaagcct
    10741 tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg aagcataacc
    10801 tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg catgtggaac
    10861 tggttatgcc attcgtctgg catagcgcgt ttgaagagtt aaaagagcaa tgtagcgccg
    10921 aactgctgcg tatcaccggg gcgaaagcta tcgactggaa actgtcgcac aatatcgcca
    10981 ccttaaaacg cgtgaagaat cagccgggta ttaatggtgt gaagaacatc attgcggtca
    11041 gttccggtaa aggcggcgtg ggtaaatcct ccacggcggt aaacctggcg ctggcactgg
    11101 cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca tcaattccca
    11161 ccatgctggg cgcagaaaat cagcgtccaa cgtcgccgga cggtacccac atggcaccga
    11221 ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac gacaatgcga
    11281 tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca gatgttgcag gaaaccttgt
    11341 ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac attcaattga
    11401 cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg caggacatcg
    11461 cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt ccggtgcttg
    11521 gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat gaaccgattt
    11581 tcggtaccgg tggtgctgag aaactggctg aaaaatacca cacacagctg ctggggcaga
    11641 tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg gttatcagcc
    11701 gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt gcagctcagc
    11761 tctactggca gggtgaagtc attccaggcg agatttcctt ccgcgcggtc taacgcctat
    11821 taaccattct ggttattctt catacaccgc agagatgtta cattgatgcg gtgtattagt
    11881 ttttgccgca ttaatgaaga cttatttaat actgcgaata ttctgcggac cttcgtccga
    11941 aactctacaa agtcgattga tcatacaggc aatgcttcat tatcatcctt cgtggctgtg
    12001 tagtggatgt acagctatag tttgttatgc actcattcta tccaggcata acctttacta
    12061 cagggataac gttcaatgaa taagtattgg ctgtcaggaa ttatttttct tgcctatgga
    12121 ttggcttctc ccgcattctc ttcagagact gccacattag cgattaatgg caggatcagt
    12181 cccccaacat gcagtatggc tatggttaat ggtcaacctc aacagcattg tggtcagtta
    12241 acgtataatg ttgacacccg tcatctgttt tcttcgcccg tcaaaggtgt aacgacagaa
    12301 gtggttgttg caggtagcga tagcaaacgt cgaatcgtgt taaatcgcta cgattaattt
    12361 ggcaacctga ttttaattgc atttagaaag ccgaaatcat ttatataaat aatttcggct
    12421 tttttattga tatcaacaat accatttaca taaatctaca ttaattgata gatgcgataa
    12481 atagttaata ctcttaaaca ttttttgtat ttatattgca atggttaatt tgttttaaat
    12541 caataaaaca atgtgtttac attgcggcaa cttttttgat gtgagaaatt tgtttccaag
    12601 agatacagac tcttaacaac caaaaataaa ttttattaag gaacatatta tgaaacgttc
    12661 aattattgct gccgctgtct tttcttcttt ttttatgagc gctggagtat ttgctgcaga
    12721 cgttgatacc ggaacattaa ctattaaggg gaatattgca gaatctccgt gtaaattcga
    12781 agcgggtggt gattcagtaa gtattaatat gccgactgta ccaaccactg tctttgaagg
    12841 taaagctaaa tattctacct atgatgatgc agtcggtgta accagcagca tgttaaaaat
    12901 tagctgcccg aaagaagttg ctggtgtaaa actctcgttg attaccaacg ataaaataac
    12961 cggtaacgat aaggagatag ccagtagcaa cgataccgtg ggttactatc tctatttagg
    13021 tgataacagc gatgtcctgg atgtttctgc accttttaac attgagagtt ataaaacagc
    13081 ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag ataactcagt
    13141 gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt aataaataaa
    13201 attgtaagtt tctccaggta ataaacatgg ccgctatccc catggcggct ttttaattta
    13261 agaggcatta agatgaaagg attattatct ttactcattt tttctatggt ccttcctgca
    13321 catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa taaagaagtt
    13381 atggtgcagt tgatgaacca gggaaaccgt tcttcgctgc tgcaggcgtg gattgatgat
    13441 ggcgatacgt cgttaccacc agaaaaaatt caggttcctt tcatgttaac gccaccagtg
    13501 gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc gaataaactg
    13561 cccactaata aagaaagcat tttttatctg aatgttctgg atattccacc aaatagtcca
    13621 gagcaagaag gcaaaaatgc actgaagttt gcgatgcaaa acagaattaa gttgttttac
    13681 cggccagcgg gtattgctcc ggtaaataaa gcgaccttta aaaagttact ggtaaatcgc
    13741 agtggcaatg gattggtgat aaaaaatgac tcagctaatt gggtgacgat ttcggatgtc
    13801 aaagctaata atgttaaagt caattatgaa actattatga ttgccccctt agaaagtcag
    13861 agtgttaatg tcaaaagtaa taatgcaaat aactggtatc tgaccattat cgatgaccac
    13921 ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag aatgacccca
    13981 cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc tgaagaaacc
    14041 tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa tattcgtctt
    14101 gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa taagcaatgg
    14161 cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt atcaatagaa
    14221 gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca atgtttaaca
    14281 tttgagcaac ttgttcaggg tgggagctat acctgggata tcggggtttt tcgtctcgat
    14341 ttcagtgtcc cgcaggcctg ggtggaagaa ctggaaagtg gctatgttcc accggaaaac
    14401 tgggagcggg gtattaatgc gttttatacc tcttattatg tgagtcagta ttacagtgac
    14461 tataaagcgt cgggtaatag taagagtaca tatgtacgtt ttaacagcgg gttaaattta
    14521 ctggggtggc aactgcattc tgatgccagt ttcagtaaaa caaataacaa tccaggggtg
    14581 tggaaaagca ataccctgta tctggaacgt ggatttgccc aacttctcgg cacgcttcgc
    14641 gtgggtgata tgtacacatc aagcgatatt tttgattctg ttcgcttcag cggtgtgcgg
    14701 ttgtttcgtg atatgcagat gttgcctaac tcgaaacaaa attttacgcc acgggtgcag
    14761 gggattgctc agagtaatgc gctggtaact attgaacaga acggttttgt ggtttatcag
    14821 aaagaggttc ctcctggccc gttcgcgatt acagatttgc agttggccgg tggtggagca
    14881 gatcttgatg tcagcgtgaa agaggcggac ggctcggtaa ccacctatct ggtgccttat
    14941 gcagcagtgc caaatatgct gcaacccggc gtatcgaaat atgattttgc ggcgggtcgt
    15001 agccatattg aaggggcgag caaacaaagt gattttgtcc aggcgggtta tcagtatggt
    15061 tttaataatt tattgacgct gtatggtggc tcgatggtcg cgaataatta ttacgcgttt
    15121 actttgggga ctggctggaa tacacgcatt ggtgccattt ccgtcgatgc cacgaagtcg
    15181 catagtaaac aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac
    15241 aaatttgtga gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta ttcatcgcgt
    15301 gattaccgga catttaatga tcacgtttgg gcaaacaata aagataatta tcgccgtgat
    15361 gaaaacgatg tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc
    15421 ttctctgcca atatgagcca gtcattgcca gaaggctggg ggtctgtgtc cttaagtacg
    15481 ttatggcgag attactgggg gcgtagcggc agcagtaagg attatcagtt gagttattcc
    15541 aataactggc ggcggataag ctatatcctc gcggcaagcc aggcgtatga cgagaatcat
    15601 catgaagaga aacgttttaa tatttttata tcgattccct ttgattgggg tgatgacgtt
    15661 acgacgcctc gtcggcaaat atatatgtct aactcaacga cgtttgatga tcaggggttt
    15721 gcctcaaata atacgggatt atcaggaacc gttggaagcc gggatcagtt taactatggg
    15781 gtcaacctga gttatcagaa tcagggaaat gaaacgacag ctggggcgaa tttgacctgg
    15841 aacgcgccgg ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacaggct
    15901 ggagccagtg tttcaggggg cattgtcgcc tggtcggggg gcgttaatct ggcgaaccgt
    15961 ctttccgaaa cgtttgctgt gatgaatgcg ccaggaatta aagatgctta tgtcaatggg
    16021 caaaaatatc gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat
    16081 cgggaaaatc acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc
    16141 aaccggaaaa ttgccgcctc ttatcgcggc gcggttgtac tggttaattt tgataccgat
    16201 cagcgcaagc cctggtttat aaaagcgtta agagcggatg ggcaaccatt aatgtttggt
    16261 tatgaagtca atgatatcca tggtcataat attggcgttg tcggtcaggg aagccagtta
    16321 tttattcgca ccaatgaagt accgccatcg gttaatgtag caattgataa gcaacaagga
    16381 ctttcatgca caatcacctt cggtaaagag attgatgaaa gtagaaacta tatttgccag
    16441 taatagtcag gtggttatga atattcgtat aatgttattt atagtaatga tgatggttat
    16501 gcctgtgagc tatgcggcat gttatagtga gttatctgtt cagcacaatt tggttgttca
    16561 gggggatttt gcacttactc aaacacaaat ggcgacatat gagcataatt ttaatgattc
    16621 gtcatgcgtg agtacaaata ctatcacccc tatgagcccg tcggatatta ttgttggact
    16681 ttataacgat accattaaat taaatttaca ttttgaatgg accaataaaa acaacatcac
    16741 gttatcaaat aatcagacca gtttcaccag tggttattcg gtaacggtga cacctgcggc
    16801 cagtaatgca aaagtgaatg tttctgcggg gggcggcggt tcagtgatga ttaatggagt
    16861 tgcgacatta tccagtgctt catcatcgac acgcgggagt gccgcagtac aatttctact
    16921 gtgtttatta ggtggcaagt catgggatgc atgtgtaaat agctacagaa atgcattggc
    16981 acaaaatgca ggtgtctatt cctttaatct gacattgtca tacaacccga taaccacaac
    17041 ctgcaaaccg gacgatttat taattacttt agacagtatt cccgtttcac aattaccagc
    17101 cacaggtaac aaagcaacaa taaatagtaa acaaggggat attattctgc gttgtaaaaa
    17161 tttattaggt caacaaaatc aaacatcacg gaaaatgcag gtgtatttat caagttctga
    17221 cttgttaacc aacagcaaca caatactgaa aggtgcggaa gataatggcg taggatttat
    17281 tcttgaaagt aatggttcgt cagtcacact tttaaatatc actaacagca gtaaacgata
    17341 cacaaattta aaagaaattg cggcaaagtc aaaacttaca gatacaacgg tttcaattcc
    17401 gataactgcc agttactacg tctacgatac aaacaaagtt aaatctggcg cactggaggc
    17461 aaccgcatta atcaacgtga aatacgacta ataacaagca agacgagcaa gtggctaata
    17521 ataaaaaata acaaggtgtc tgcatttccc cctcatgatg agggggcttt ttttagcgat
    17581 gataaaaaat ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg ctgatcaaca
    17641 cgtacgcgcc acccatatag gtccagtgcg tcccggcatc aggcgcgggc aggttacgta
    17701 actgccactg cttaatgttg tactcatcgg tgcggtacat ctctgggacg gtatcgcctg
    17761 gtttgaaatg cttaaagtca gcaatgaaac tgcttaactc atacttagaa attcccgagg
    17821 tattggtcga acccgcggtg gctggtgcgg cccaggcggc actggtaacc agcaaaagcg
    17881 cacccagcaa catcttattt ttaatggtca taaactctcc actttgggga tctgtaatat
    17941 cagtcgcata tctttcctgc catacaatat aactgacaag cgatattttg ttagtgatcc
    18001 catgatttcg taaaaagtgt aaatgaaagg atgccctaaa agcatcctta gcgagcagag
    18061 agtcagcgga aatagctgat ttaagaatca tcctgggctg aagataacat gcaagcctaa
    18121 aggattgagt tatcgcatta tgcccatgaa gccatgtacg cccatataca caccgactaa
    18181 gccaatcaac agactggaaa aatagggggc gcgtttagcg agagtgttaa atccgctcca
    18241 gcgttttgcg acctgctgaa cgctgattgc tgcgccaacg cctacggtga caagcgttaa
    18301 cgccaggcca atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg
    18361 aatgcatatc aacagcacgg taattgctgc cgggcagggg ataaggccac cggttaagcc
    18421 aaataacaaa atttgccagt tggtgacctc tctaccatca aagcgtcgtt taatgtcatt
    18481 agcatgggct cgtgcatgag catcctgata ctcgccatgt tcgtgatggt gatgatgtcc
    18541 atggtcgtgg tgatcttcgt gatcgtgatg atgatgctca tgatcatgct cgtgcatatt
    18601 ctccagccag ttgcgctcgc cgtgccaggt acgccagaac atccagaacg cggtgctaat
    18661 aatgatcact gcggaaatca actggagcca cggttctgct gattgagcag taaagcgctt
    18721 gctgaccacc atcccgccaa aggcaatcaa ccagaccact gcggtatgcg aaatagttgc
    18781 tgccagtccg agcatcaccg cctgtttaat ggtgcctttg atggcgatga taaacgccgc
    18841 catcatcgtt tttgagtgcc ctggttccag gccatgaagc gcaccaagta agatggcgct
    18901 ggggatgaag aaccaggcgt ttccttgctg aagaagagtt gtaaattcgg tcatgataat
    18961 aattcttagt attaattcgg caatctgatt ctactccccc ccagtacctg atactacccc
    19021 ccagtagatt aatagtgcta tgatttttca tattcttgtt aaccaggtgt tgccatgtct
    19081 catacaatcc gtgataaaca gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc
    19141 gtggcgctca agaaaatgct cgacgaaccg cacgaatgcg ctgcagtttt acaacagatt
    19201 gctgctatcc gtggcgcggt aaacggtctg atgcgggaag tgattaaagg tcatctgacg
    19261 gaacacatcg ttcaccaggg ggatgagcta aaacgtgaag aagatctgga tgtcgttctg
    19321 aaggtgctgg attcatatat caaataattt attaacgcga ttgtaaaact gccgtttttc
    19381 ctcgtttaca acgcgtgcgc tggacattac catcctcctc tgcgatttat catcgcaacc
    19441 aaacgactcg gggtgccctt ctgcgtgaag gctgagaaat acccgtatca cctgatctgg
    19501 ataatgccag cgtagggaag tcacggacca ccaggtcatt gcttcttcac gttatggcag
    19561 gagcaaacta tgcaagtcga cctgctgagt tcagcgcaat ctgcgcacgc gttacacctt
    19621 tttcaccaac attcccctct tgtgcactgc atgaccaacg atgtggtgca aacctttact
    19681 gccaatacct tgctggcgct cggtgcatcg cctgcgatgg ttatcgaaac cgaagaggcc
    19741 agtcagtttg cggctattgc cagtgcgttg ctgattaacg ttggcacgct gacgcagcta
    19801 cgcgcacagt cgatgtgtgc cgccgttgag caagcaaaaa gctctcaaac accctggacg
    19861 cttgatccag tagcggttgg tgcactcgat tatcgccgcc gtttttgtct ggaacttctg
    19921 tctcataagc caaccgccat acgtggtaat gcttcggaaa tcatggcatt agctggcatt
    19981 gctaatggtg gacggggagt ggataccact gacgccgcag ctaacgcgat acccgctgca
    20041 caaacactgg cacgggaaac tggcgcaatc gtcgtggtca ctggcgagat ggattatgtt
    20101 accgatggcc atcgcgccgt tggcattcac ggtggcgatc cgttaatgac caaagtggta
    20161 ggaactggct gtgcattatc ggcggttgtc gctgcctgct gtgcgttacc aggcgatacg
    20221 ctggaaaatg tcgcatctgc ctgtcactgg atgaaacaag ccggagagcg cgcagtcgcc
    20281 agaagcgagg ggccaggcag ttttgttcca catttccttg atgcgctctg gcaattgacg
    20341 caggaggtgc aggcatgaaa cgaattaacg ctctgacgat tgccggtact gatcccagtg
    20401 gtggtgcggg gattcaggcc gatcttaaaa ccttctcggc acttggcgct tatggttgct
    20461 cagttattac tgcactggtg gcgcaaaata cccgtggcgt acagtcggtg tatcgcattg
    20521 agcctgattt tgtcgccgcc cagctcgatt cggtgttcag cgatgtgcga atcgacacca
    20581 ctaaaatcgg tatgttggcg gaaaccgata ttgttgaagc ggtggcagaa cggttgcaac
    20641 gttatcagat ccaaaacgtg gtactcgaca ccgttatgct ggcaaaaagc ggcgacccgc
    20701 tgctttcacc ttcggcggtt gctacgctgc gcagtcgatt attgccacag gtttcattaa
    20761 taacgccaaa cttgcccgaa gctgccgcct tgctcgacgc gccacacgcg cgcaccgaac
    20821 aggaaatgct ggaacaaggg cgatcgctgt tggcgatggg ctgtggcgca gtgctaatga
    20881 aaggtggtca tctggatgat gagcaaagcc cggactggct gtttacccgc gagggtgaac
    20941 aacggtttac cgcaccgcgc attatgacca aaaacaccca cggcactggt tgtacgctct
    21001 ctgcggcgtt ggctgcacta cgcccgcgcc atacaaactg ggctgacacc gtacaggagg
    21061 caaaaagctg gctttcatca gcattagccc aggccgacac gctggaagtt ggtcacggta
    21121 ttggtccggt tcaccacttc cacgcctggt ggtgagcacc gtactatagc tgctttggtt
    21181 attgtcagcc tttggcactg acattcaggg atgaaaccaa tgcagctaag aattaccagt
    21241 cgaaaaaaac tcacctctct attatgcgct cttgggctaa tttccatcgt tgcaatttat
    21301 ccgcgtcaga cggtgaattt tttctattcg acagcagttc agattacaga ctacatccat
    21361 ttctacggtt atcgcccggt taaatctttc gctattcgca ttcctgccag ttacaccatt
    21421 cacggaatag atgtttctcg ctggcaggag cggatcgact ggcagcgggt ggcaaaaatg
    21481 cgcgacaacg gtatccgctt acagtttgct tttattaagg cgacggaagg cgaaaagctg
    21541 gtggacccgt atttttcgcg taactggcaa ctaagccgcg aaaatggcct gctgcgcggg
    21601 gcgtatcatt atttctcccc gtcggtagca gcgccggttc aggcacgatt atttctgcaa
    21661 acggtggatt tctcacaagg cgatttccct gcggtactgg acgtagaaga acggggaaaa
    21721 ttatcggcaa aagaattacg caaacgagta agtcagtggc taaaaatggt cgaaaaaagt
    21781 acgggaaaaa agccgattat ttactcagga gccgtttttt atcacaccaa tctggcgggc
    21841 tatttcaatg aatatccgtg gtgggtggct cactattatc aacgtcgtcc ggacaatgac
    21901 ggcatggcct ggcgcttctg gcagcattcc gaccgtggac aggtagatgg catcaatggt
    21961 ccggtggatt ttaatgtatt tcatggcacg gtggaggagt tgcaggcatt cgttgatggg
    22021 attaaagaaa cgccttaaat aagaagcagt gatatactcc caccagactc cccggcagga
    22081 aaaagacaaa tggaacaagc gcatacccag ctgattgccc aactgaacga gcgtatttta
    22141 gcggcggata acacgccgct ttatattaag tttgccgaaa cggtaaaaaa tgccgtgcgc
    22201 agcggggtgc tggagcatgg caatattttg cccggtgagc gtgatttaag tcagttaacc
    22261 ggcgtttcgc gcattacggt gcgcaaggcg atgcaggcgc tggaagaaga gggtgtggtg
    22321 acgcgttcgc gcggttacgg cacgcagatc aacaacatct tcgaatactc gttgaaagaa
    22381 gcgcgaggtt tttctcagca ggtggtattg cgcgggaaaa agcccgatac gctatgggtt
    22441 aacaagcgtg tcgtgaaatg tcccgaagaa gtcgcgcagc agctggcggt cgaagcagga
    22501 agtgatgtct ttttgcttaa gcgtattcgc tatgtcgatg aagaagcggt atcgattgag
    22561 gaatcgtggg ttccggcgca tttaattcat gatgttgatg ccatcgggat ttcactttat
    22621 gactatttcc gcagccagca catttaccca caacgtacgc gttcccgcgt tagcgcccgg
    22681 atgccggatg ccgagtttca gtcacatatt cagttagata gcaaaatacc ggtgctggtg
    22741 atcaagcaag tggcgcttga ccaacagcaa cggcctattg agtacagcat tagccactgt
    22801 cgcagcgatc tatacgtttt tgtgtgcgag gagtagttcc tcgcgcgttg gcgcacaatc
    22861 accgccccga tgcccgacaa cccacgacgc cactgcattg cccagtaata cggcatccgc
    22921 cagtggcaga ccagaggcca gcccggcaag tacgccacct gcatgactgt cgcccgcccc
    22981 aatggtgtct acaacttgcg ttggaaatgc cggaatgcag ccagaagcgt cgttgctgaa
    23041 ataccatgca ccctctttat cgagacgaac gatcaacggc gcggcaaatt tctcctgcca
    23101 ttgcttgcca agtgttgtta tctctgcgga taaagcaaaa cgttcggcgg caatctcagc
    23161 ctcttgacga ttgagtgata ctaaaggtcg acaggccatg atccgcgcca gtaatgcgtc
    23221 cgggatatcg ccgatacgtg ggccaaaatc gataaacggc gtcacgtctt gcagcttttc
    23281 cagccattcc actaacaatt cgccgcaggg cgaggccagt tgataaccgg aaaaatagag
    23341 caggctgcca ggcgcaacgg ttaatcgcgc cagccactgg cgattccact gattttccac
    23401 accgctgaat gacataaaag tgcgttcgcc atccggctca accagcgcca gacaccagcc
    23461 gttatcgcct tcggcgttat cgatcaggct gattaacccc tctttcgcca tccggttgcg
    23521 aataatctcc gcccacacgc cctgaccgag cggcaaggca ttacccgctt cgatgccgag
    23581 gcgctttaac gccacggcaa tattcagtgc gcagccgcca acgttaacgc tctgctgctt
    23641 cagttcgata tcgcacccac gccaggggag ggcataagcg tcggcgatca catcaatgac
    23701 cgccgcgccg acgaccatca ccggctgacg cgaggttaat tcaggcagca gcgtgtgtaa
    23761 tcgagcgccg ctcataccgc ctcccgttgt tgacgatatt tcgccagcgc tgtggcatag
    23821 cggttgaaat caagctgatt taccgcatcc agttccgcct ttaatgcagg atcgatagcg
    23881 ttaacgccat gcaacgcgcc gcaaatcgcc gtcgccatag cgccgatggt gtctgtgtcg
    23941 ccgccaaggt tagcgcacag gacggcgcag cgattcgggt cggtttgtgc cagttcaacc
    24001 agcgcaatgg cgcacggaac ggactcaata gtgctggtac ctgcgccaac gacctggtaa
    24061 agctgttcgc tggcggattc ggtgccgtcg gcattgcgca caattttcag cgcaatctcc
    24121 agacgtgctg ccagtgaggc gctgaaggta gtgatgcgtt tttgctgtgc atgtcgcgca
    24181 attgaaggca gggaatcaac aatcgctgac cagctttctc cgtcaatggc acgagaaatc
    24241 gcccatgcga tgactaccgc acctgcaact gccagatcgg atttatgggt cgggctcgac
    24301 gccagcgcca catcatcaat aaaggaatca acatcacgcg ccggaagcaa acaacctaat
    24361 ggcgagacgc gcatcgctgc gccgttggtc acgccgttgt tttccagttc agcaacgggt
    24421 ttaccgtcgc gaatggcgtt aagcgcaatc ttcgaggtcg gacctagtac gtttttgtta
    24481 aaggcgtcga aacgcagcgc ccagtcgaga atattacgcc caatcagatc cggatcgatc
    24541 ttgccttcac gttccagtaa cgcatccgcc agacacagcg ccatcgaggt atcgtcggtg
    24601 aattcggcgc ggttaaaata acaggccgcg ttattctcct ttggtccagg cagaaaacgg
    24661 tcaatccagc caaagtgtgc tttaacgcgg ctgcgtggcc aaagctcgga gggcatcccc
    24721 atcgcatccc ctaacgcctg cccataaaga gcaccgagaa tacgttctgt tttcatttaa
    24781 cttccccttg tgtcaacgca atatcgcgat catcgacctt gatagccgta atttcgttgt
    24841 cggattcgcg gaaaaaaatc atgaacagca cggcgataat ggcaatcatc accgcgccga
    24901 aagtccacat cccggaccag ttgaaagtca gcccgtttac cggttccgga taagcgaaca
    24961 tcttttccat catcacaccg ccaagacgat agccgagcaa actgccgaag ccctggcagc
    25021 agagcgtgat cagcccctgc gcggcggtac gcatatgcac gggggctttt ttatcgacat
    25081 agatgtaagc ggtaacgtag taaaaatcgt aacttacgcc gtgaagcaaa ataccgagga
    25141 acagtaacgc gtaggtgaaa tattcatccg cactaccgta aataaagaag ccatagcgga
    25201 tcgcagcggt gaccagacca agcaataata cctttttgat accaaagcgt ttagtgaaaa
    25261 acggcaatgc cagcataaag aagatttcag agaactggcc gagcgtcatc cagccggtgg
    25321 cgtttttcat gccaacttcg gtcagataac cgttggcaaa gatgtaatag aacgccagtg
    25381 gcatcgcaaa caggaatgaa cagaagaaaa agacgaggaa gtttttatcg cgcagcagga
    25441 tcagcgcatc caggccgagc atgactttaa tgtccatttt gccggtgctt tttggcggcg
    25501 tgtcgggcag gaaaaacgca aacacaccga gcagagcaga acttccggcg gtaatcagca
    25561 gcgggatgtt agtcggtgag atatcggcat accccagcat ttgcggcaag aaaccacatg
    25621 ccagaccaga ggcaatccag ccgatagtgc ccatcacacg aatgcgcggg aaatcacgct
    25681 ccacatccgg cacgttggca aaagcgatac tgttagtcag cgcaatggtc ggcatatagg
    25741 ttagcgagta ggccagcagt aacgggaaga acccggcaaa agtggtctgt tgcgcagcga
    25801 aatacatcag cagcgcgcct gcgaacatca ataccgccag cactttttgc gccgagaaaa
    25861 agcggtcagt gatggagcca accagaattg gcgacaggat cgccgcaatg gctgtgcagg
    25921 catacgacca gccaatttct ccggcactaa aaccgctttt acttaaccac aaccacaatg
    25981 gcacaaacca cgcgccccag ataaaccatt caacaaacat catgaacgac agctttgctg
    26041 ttgttttcat cttttaattc cttcatgaca ggtaatgtaa ggtacgcgat gacaatacca
    26101 cttaataata ccttttaaat acctttgcgg tgaaaacttg accgcgctaa cattttttct
    26161 gatgaatcga gccaacagaa aacgctgaaa aaacatccaa aagatggaaa aactcgcccg
    26221 taacgaccag gattatgatt gcccgcaaaa ggcgggccag gacaatccat agccgatatc
    26281 caatcggaat ttacgggagc atagtaatga cagatattgc gcagttgctt ggcaaagacg
    26341 ccgacaacct tttacagcac cgttgtatga caattccttc tgaccagctt tatctccccg
    26401 gacatgacta cgtagaccgc gtaatgattg acaataatcg cccgccagcg gtgttacgta
    26461 atatgcagac gttgtacaac accgggcgtc tggctggcac aggatatctt tctattctgc
    26521 cggttgacca gggcgttgag cactctgccg gagcttcatt tgctgctaac ccgctctact
    26581 ttgacccgaa aaacattgtt gaactggcga tcgaagcggg ctgtaactgt gtggcgtcaa
    26641 cttacggcgt gctggcgtcg gtatcgcggc gttatgcgca tcgcattcca ttcctcgtca
    26701 aacttaatca caacgagacg ctaagttacc cgaataccta cgatcaaacg ctgtatgcca
    26761 gcgtggagca ggcgttcaac atgggcgcgg ttgcggttgg tgcgactatc tattttggct
    26821 cggaagagtc acgtcgccag attgaagaaa tttctgcggc ttttgaacgt gcgcacgagc
    26881 tgggtatggt gacagtgctg tgggcctatt tgcgtaactc cgcctttaag aaagatggcg
    26941 ttgattacca tgtttccgcc gacctgaccg gtcaggcaaa ccatctggcg gcaaccatcg
    27001 gtgcagatat cgtcaaacaa aaaatggcgg aaaataacgg cggctataaa gcaattaatt
    27061 acggttacac cgacgatcgt gtttacagca aattgaccag cgaaaacccg attgatctgg
    27121 tgcgttatca gttagctaac tgctatatgg gtcgggctgg gttgataaac tccggcggtg
    27181 ctgcgggcgg tgaaactgac ctcagcgatg cagtgcgtac tgcggttatc aacaaacgcg
    27241 ctggcggaat ggggctgatt cttggacgta aggcgttcaa gaaatcgatg gctgacggcg
    27301 tgaaactgat taatgccgtg caggacgttt atctcgatag caaaattact attgcctgat
    27361 acgcctcata cgaacagcac caccccgtag gacggataag gcgtttacac tgcatccggc
    27421 aacctatgcc tgatgcgacg cggaagcgtc ttatcatgcc tacatagcac tgccacgtat
    27481 gtttacaccg catccggcat aaaaacacgc gcactttgct acggcttccc tatcgggagg
    27541 ccgttttttt ttgcctttta ctcctcgaac aattttcaca ttgtcgcttt tgtgatcgtt
    27601 atctcgacat ttaaaaacaa atcatttcat tatattttga aatcgaaaac aaacgacagg
    27661 atatgaaaat gtacgtggta tcgacaaagc agatgctgaa caacgcacag cgcggcggtt
    27721 atgcggttcc ggcattcaat attcacaatc tcgaaacgat gcaagtggtg gtggaaaccg
    27781 ctgccaacct gcatgcgccg gtcatcatcg ccggaacgcc tggcacattt actcatgctg
    27841 gtacagaaaa tctgttggcg ctggtcagcg cgatggcgaa gcaatatcac catccactgg
    27901 caattcatct cgaccatcac acgaaatttg acgatatcgc tcagaaggtt cgttctggcg
    27961 tgcgctcagt catgattgac gcctcgcatt tgccttttgc gcaaaatatt tcacgggtca
    28021 aagaggtggt ggatttttgc catcgctttg atgtcagcgt cgaagcggag ctggggcaac
    28081 ttggcggcca ggaagatgat gtgcaagtca atgaagccga tgctttttat accaaccccg
    28141 ctcaggcgcg tgaatttgcc gaggcaaccg gaattgattc cctggcggtc gccatcggca
    28201 cggctcatgg gatgtatgcc agcgcaccgg cgcttgattt ttctcgactg gagaacattc
    28261 gccagtgggt gaacttaccg ctggtgctgc atggcgcgtc agggttatcg actaaggata
    28321 ttcagcaaac catcaaactg gggatatgca aaatcaacgt tgcaacggag ctgaaaaatg
    28381 ccttctcgca ggcgttaaaa aattacctga ccgagcaccc tgaagcgacc gatccccggg
    28441 attatttgca gtcggctaaa tccgcaatgc gcgatgtggt gagcaaagtg attgccgatt
    28501 gtggctgcga gggcagggca taacgcacct gccatttaat aaggaaaaaa catgaaaacg
    28561 ttaattgccc ggcataaagc tggtgaacat atcggcatat gttcagtctg ttctgcccat
    28621 ccgttggtta tcgaagcggc gctggcattt gatcgcaaca gcacgcgcaa agtgctgatt
    28681 gaagcaacgt caaaccaggt caatcaattt ggcggttata ccggaatgac accggcagac
    28741 tttcgcgaat ttgtttttac gattgccgat aaagttgggt ttgcacgcga acgcattatt
    28801 ctcggcggcg atcatctggg gccaaactgc tggcagcaag aaaatgcgga tgcggcgatg
    28861 gaaaaatccg tcgagctggt aaaggaatat gttcgtgccg gtttcagtaa aattcatctt
    28921 gatgcgtcaa tgtcctgcgc gggggatccc ataccgttag caccagaaac ggttgcggaa
    28981 cgagctgctg tgctttgctt tgctgcggaa agtgtggcga cagattgcca gcgtgagcaa
    29041 ctgagctatg tcattggcac cgaagttccg gttccgggcg gtgaggccag cgccattcag
    29101 tcagtacaca tcacccatgt tgaagatgcc gccaatactt tacgtacgca tcaaaaggcc
    29161 tttattgccc gtgggctgac agaggcgtta acacgcgtga ttgccatcgt ggtgcagccg
    29221 ggcgtggaat ttgatcacag caatattatc cattatcagc cgcaggaagc gcagccgctg
    29281 gcgcaatgga tagaaagcac ccgaatggtt tatgaagcac attctaccga ttaccagacc
    29341 cggacggctt attgggaatt agtccgcgat cactttgcaa tattgaaagt cggtcccgca
    29401 ttaacctttg ctttacgcga ggcgatattt gcactggcac aaattgagca ggaacttatc
    29461 gcccctgaaa atcgcagcgg ttgcctggcg gtaattgaag aagtgatgct cgacgaaccg
    29521 caatactgga aaaaatatta tcgtacgggt tttaacgatt cattactgga tattcgttac
    29581 agcctgtcgg atcgtattcg ttattactgg ccgcatagtc ggattaaaaa tagcgtcgaa
    29641 acgatgatgg tgaatcttga aggcgtggac atcccactgg gcatgattag tcagtatctt
    29701 cccaaacaat ttgaacgcat tcagtccggg gaattatcag caataccgca tcagctgatt
    29761 atggataaaa tttatgatgt tttgcgcgcc tatcgctacg gctgtgcgga ataaggacgg
    29821 tatatgacta acctgtttgt tcgtagcgga atttcttttg tcgatcgtag cgaagtttta
    29881 acccatatcg gtaatgagat gctcgcgaaa ggtgtggttt atgatacctg gccacaggca
    29941 ttaattgcca gagaagcaga attccctacc gggataatgc ttgagcagca cgccattgct
    30001 ataccgcatt gtgaggcgat tcatgctaag tcgtcagcca tttatctgtt aaggccaaca
    30061 aataaagttc attttcagca agcggatgat gataacgacg tggaggtgtc gttggttatt
    30121 gcgttgattg tggaaaatcc gcagcagcaa ttgaaacttt tacgctgttt atttggcaag
    30181 ttacaacagc ccgatatcgt cgagacacta attactcttc ctgaaactca gttaaaggaa
    30241 tacttcacaa agtatgtttt agattcagac gaataaatcc ctctgtaaca ataaaaagga
    30301 ctatttatga aacgcaagat tattgtcgct tgcggaggcg cggttgcgac ctctacgatg
    30361 gcggcggaag aaattaaaga gttgtgtcag aatcataata ttcctgttga attaatccag
    30421 tgtcgggtta atgaaataga aacctatatg gatggtgtgc atttgatatg caccactgcc
    30481 aaagtggatc gtagttttgg cgatattccg ttagttcacg gcatgccttt tatttctggt
    30541 gtcggtatcg aagcattaca aaataaaatt ctgactatct tacaggggtg acctatgttt
    30601 tcagaagtca tgcgttatat tctcgacctc ggccctacgg tgatgcttcc gattgtcatc
    30661 attatttttt ctaaaatatt aggcatgaag gcaggcgatt gctttaaagc gggtctgcat
    30721 atcgggattg gctttgttgg cattggcctt gtgattggct taatgctgga ttccattggt
    30781 ccggcggcga aagcgatggc ggaaaatttc gacctgaatc tgcatgtggt cgatgtcggc
    30841 tggccgggct cttcaccaat gacctgggcg tcgcaaattg cgctggtggc gattccgatt
    30901 gcgatattgg ttaacgtggc gatgttactg acccgtatga cgcgggtggt aaatgttgat
    30961 atctggaata tctggcatat gaccttcacc ggcgcgttgc tgcacctggc aaccggttca
    31021 tggatgatag ggatggcagg tgtggtaatt cacgcggcgt ttgtttataa gctcggcgac
    31081 tggtttgccc gcgatacccg aaatttcttt gagctggaag gtattgctat tccgcacggt
    31141 acgtcggcgt atatggggcc gattgcggtg ctggtcgatg ctatcatcga gaaaatccca
    31201 ggcgttaacc gaattaaatt tagcgccgac gatattcagc gcaaatttgg tccatttggc
    31261 gagcctgtca ccgtgggttt tgtgatgggg ctgattatcg gcatcctcgc gggttacgat
    31321 gtcaaaggtg tattgcagct ggcggtaaaa acggcggcag tgatgctgct aatgccacgg
    31381 gtgattaaac ccatcatgga tggtttaacg cccatcgcta agcaggcccg tagtcgttta
    31441 caggcgaagt tcggcggtca ggagttcctg attggcctgg atcccgcatt actgctgggg
    31501 catacggcgg tggtatcggc aagcctgatt tttatcccgc tcaccatttt aattgctgtt
    31561 tgtgttccgg gtaatcaggt gctgccgttt ggcgatcttg ccaccatcgg cttctttgtg
    31621 gcgatggcag tcgccgtgca tcgtggaaat ctgttccgca ccttaatctc gggtgtcatc
    31681 atcatgagca tcactctgtg gatcgcgacg caaactattg gtttgcacac ccaactggcg
    31741 gctaatgctg gggcgttaaa agccgggggt atggtggctt caatggatca gggcggttct
    31801 cccattacct ggttactgat tcaggttttc tccccgcaaa atattcccgg attcattatt
    31861 atcggcgcaa tttatcttac cggtattttc atgacctggc gtagagcgcg tggctttatt
    31921 aaacaagaga aagtcgttct cgcagaataa tttttacctg agggggaggt tatccccctt
    31981 aacaatcagg agtttttatg aaatcagtgg tgaatgatac tgatggtatc gtgcgcgttg
    32041 cagaaagcgt cattcctgaa attaaacatc aggatgaggt gcgggtaaaa attgccagct
    32101 cgggcttatg tggttccgat ttacccagga tatttaaaaa tggtgcacat tattatccaa
    32161 taacgttagg ccatgaattt agcggctata ttgatgcggt gggatccggt gttgatgatt
    32221 tacatcctgg cgatgcggtt gcctgtgtgc cgttattacc ctgttttact tgtccagagt
    32281 gtttgaaagg gttttattcc cagtgcgcaa aatatgattt tattggctca cggcgtgatg
    32341 gtggatttgc cgaatatatt gtcgttaagc ggaaaaatgt ctttgctcta cctactgata
    32401 tgcctattga ggatggggct tttattgaac cgattaccgt tggcctgcat gcttttcatt
    32461 tagcgcaagg ttgtgagaat aaaaacgtta ttattattgg tgccggaacc attggcctgc
    32521 tggccattca gtgcgctgtc gcgctgggag caaagagtgt gacggcgatc gacattagtt
    32581 cagaaaaact ggcactggca aaatctttcg gtgcgatgca aacatttaac agtagcgaaa
    32641 tgagcgcgcc gcaaatgcag agcgttttac gctaactgcg ctttaatcag cttatcctcg
    32701 agacggctgg cgtaccgcaa actgtcgaac tggcggtaga gattgccggt cctcatgccc
    32761 aactggcgct ggtgggcacg ttgcatcagg atctgcattt aacatcggca acgtttggca
    32821 aaatattgcg taaagagctg acggttatcg gcagttggat gaactactcc agcccttggc
    32881 cggggcagga gtgggaaacg gcgagccggt tgctgacaga acgtaagtta agcctggagc
    32941 cattaatcgc tcaccgtgga agctttgaaa gcttcgccca ggcggtgcgt gacatcgctc
    33001 gtaatgctat gccgggcaaa gtgttgctca ttccctgaaa ccgcgggcca gcgtgatgct
    33061 ggcccggtat tgtgcaaaac agatcattca ccaatggtcc cccttcgttt acactagcca
    33121 caattgaata tgggtaaatg acgatgaatt cattcgagcg aaggaataag atcatccaat
    33181 tagtgaatga acagggaacc gtgcttgttc aggatctggc gggagtattt gctgcctcgg
    33241 aagcgacaat ccgtgccgat ttgcgctttc tcgaacaaaa aggcgtggtt acgcgctttc
    33301 atggcggtgc ggcgaaaata atgtccggta atagtgaaac cgagacccag gaagtcgggt
    33361 ttaaagagcg atttcagctc gccagcgcgc cgaaaaacag aatagcgcag gcggcagtca
    33421 aaatgatcca tgaagggatg accgttattc tcgacagcgg aagtaccaca atgcttatcg
    33481 ccgaaggatt aatgaccgcc aaaaatatca cggtgattac caacagtctc ccggctgcgt
    33541 ttgccctttc cgaaaataaa gacattactc tggtcgtctg cggtggcacg gtccgccata
    33601 aaacgcgatc tatgcatgga tctattgctg aacgttccct gcaggatatt aatgccgatt
    33661 taatgtttgt cggtgctgat ggtattgatg cggttaatgg tattacgacc tttaatgaag
    33721 gttattcgat tagcggtgcg atggtcaccg ccgctaataa agtgattgcc gttctcgatt
    33781 catcgaaatt taaccgtcgc ggttttaatc aggtactgcc aatcgaaaaa attgacatta
    33841 ttattaccga tgatgctgta tcagaggtgg ataaactggc attgcagaag acacgggtaa
    33901 aattaattac ggtgtagtat tgttctgtat gccggataag gtatttacac cgcatccggc
    33961 atttcggatt ctccctggtt caagagagaa tagtttgatt aacgcaataa tggacaatct
    34021 ggtggtaatc gacaacgcaa cgccgccgga agtatttcaa tatgaaagtt ttgtccgctc
    34081 aacggttcgc catcaagatt aaaagtgatt tcgtgtgggg cttgtatatc aaaccacgac
    34141 gaagcgcctt cgataatatt cgggttatct tcgtcagatt ttaaggttga tacgagagcc
    34201 ggaagaattt catcgccggt aaaaatgcgc agttgcagca agccatcgtt aattaacgcg
    34261 ttcggacaca attgctgacc gccaccggcc tgacgcccgt taccaatacc aatgaccagg
    34321 gcgtcacctt gccagtgaaa gttttcaccg cggatttcac aacggtccgg ttgcagagta
    34381 tccatgcgca ttaagccatg aatgatgtaa gagacgccac ccagcgcggc ttttaatttt
    34441 tccggcgttt ctgtggtaat acgcgtccca aatccgcctg tcgccatatt aataaaacag
    34501 gtttgtttgt tgacctgcgc catatctatc gcaatggcgt taccggcaat tgccagtttc
    34561 agcgccttat ccagtgcctc aggaatccct acactggtgg caaaatcatt ggcggttcct
    34621 aatggcaaaa ttcccagcgc gggtatgtca tccccctcac actgaatcaa cgccgtagaa
    34681 acttcattaa tggtgccatc gccaccaccg gcaatcaccg ttgcgacgcc caacttccgg
    34741 gcctcctcta catatcgtgc ggcatcgcct ttctcccagg tgacccgcac atggatcgtc
    34801 attccttcct cacgcaacag cataattgct tcgcgcaagg gtagattgtc agtacttttg
    34861 ccattaagaa tcagtaagct ggcgggaaat tctgccatga tcgtatgtgc ctttatgatt
    34921 ggtctgtaga tagtgtagag cagaaaacaa aaggtggagt cagaacagga tgaaagtcgg
    34981 aggataaaaa gtcagttcaa gtatggagga ttcagtggtt accaccagga gcttgcaatg
    35041 gggacagcga atactaacgg ctgccaccga taaatttcaa aaaagagcat atgcctaata
    35101 ttcaaccaaa cagtggcatc ttaaatatac tattccaaat atattaaagc ccccatggag
    35161 ttaccctgaa gggcctcaat gtccgtaatt cctacttatg taggaaatgt tgtacagaac
    35221 atttattata atcctattca attataataa tcatgccatt attatattta aacactagag
    35281 agtgtcgttg gtatttaatg ggggaaggtg agatgaaaaa gatagctgct atatcattaa
    35341 ttagtatttt tattatgtct ggttgtgctg tgcataatga tgagacaagt atcggtaaat
    35401 ttggtcttgc atataaaagt aatattcagc gtaaactcga taaccaatac tacaccgaag
    35461 ccgaagcttc tttagccagg ggcagaatat ctggtgcaga aaatatagta aaaaatgatg
    35521 cagcccattt ctgtgttact cagggcaaaa aaatgcagat agttgacctg aagacagaag
    35581 gtgcaggatt acatggcgtc gctcgtctga cattcaaatg tggagagtga gaatattttt
    35641 tggtaagcgt caaacatgcg cgttctggtt gtgcttagcc ggaacctgtg cgagcacgat
    35701 gccgttacgt gaaaggcatc gtgctatgaa gggagattct atcgatgtgg tcaatggaag
    35761 acggtgacca gggatagggc ttatgcataa aaaataagcc cgtgtaaggg agattacaca
    35821 ggctaaggag gtggttcctg gtacagctag cattttatgg gttatgtttt tcagcgaaac
    35881 ggatgataac cttaataaat gcagctgtat gtgatcggtt tctaagaatt ttccatccgg
    35941 gaaaaataat cgaaattaat cacttaccgt ggggattacg cgtggtttcc ccggagaaat
    36001 tacgcatcag cagcgcgtaa ttgagctcaa gatcctgcgg gaccgggagc cacacagtat
    36061 aaccatcgcc tggtgctatc ggcatagctt cgcctttggc gttttccatg tgctcaaggg
    36121 taaaattaat gttgccttgc ggcgtcatca gctcaaggct gtcgccaacg gagaatttat
    36181 ttttcaccgc taccgccgcg aggtccccct tgcgctcacc ggtaaactca ccaacaaact
    36241 gctggcggtc agaaactgaa taaccgtatt cgtagttctg ataatcgtcg tgagtatgac
    36301 gacgcaggaa accttcggta tagccacgat gcgccagacc ttccagagtt tccagcaggc
    36361 tggtatcgaa cggttttccc gcagcggcgt catcgatagc tttgcggtaa acctgtgcgg
    36421 tgcgtgcaca atagtagaaa gatttggtac gaccttcgat tttcagcgaa tgcacgccca
    36481 ttttggtcag gcgttctaca tgggcgatgg cgcgcagatc tttcgagttc atgatgtaag
    36541 tgccgtgctc atcttcaaac gcggtcatat actcgcccgg acgctgggcc tcttcgatca
    36601 taaacacttt gtcggttggt gcgccgatac ccagcgtcgg ctcaacattt tgcaccggaa
    36661 tcggctcgta cttgtgtacg atgttgccaa catcatcttc tttcccttcc tggacgttgt
    36721 actcccagcg gcaggcgttg gtgcaggtgc cctggttcgg gtcgcgcttg ttgatatagc
    36781 cagagagcag gcagcgaccg gagtaggcca tgcacagcgc gccgtgaacg aagatctcga
    36841 tctccatatc cggcacctga ttgcggatct cttcaatctc ttccagcgac agctcgcgag
    36901 agaggatcac gcgggtcagg cccatttgct gccagaattt caccgtcgcc cagttcacgg
    36961 cgttagcctg caccgaaagg tggatcggca tttcagggaa gtgctcacgc accagcataa
    37021 tcagccctgg atcggacata atcagcgcat ccggccccat ttccaccacc ggtttcaggt
    37081 cacggataaa ggttttcagc ttggcgttgt gcggtgcaat gttgaccacg acataaaact
    37141 ttttccccag cgcgtgggct tcattgatgc cgagctgaag attttcgtgg ttgaattcgt
    37201 tgttgcgcac acgcagggaa taacgcggct ggcccgcata aacagcatct gcgccataag
    37261 cgaaagcgta acgcatattt ttcagcgttc ccgccgggga aaggagttcc ggtttaaaca
    37321 taattctctc gttctgatga caggtcagat ccgcttcacc tgatgaagcg gttaggggag
    37381 tgcccccact ttaagggcgg gcattgtagc gcaatcggcg ggtttagctc cagtgtaaat
    37441 gtctggtggc gtcctggttt tggttaagga tacgaatgac gataatttcc cctggtgatt
    37501 gcaggaaata aatgacgtgg cgttcaaaag gaagaacaaa aataccttcg cctaattcag
    37561 gtctgggcgt acctatatta ttgttactca gaatctggag aacgtctgaa agatgttcca
    37621 catatctgtc ggcttgtggt tcgccgaaat ggtgatagct gtaataccag atgccttcta
    37681 aatcctcatt ggcttttggc atgagtttaa taatacgcac attatttcct tatccctgct
    37741 ttcaccttcc gtaagaacgc gtccttttcc cacggctgag cttcaccact actcaaccct
    37801 tctgcgagca tgtcacgtaa cgcttgcagg cgagactctg cttgcttttc tcgaagtaat
    37861 cgcagtgact cacggatcac ttcgctttgg gtgcgatagt cgccggattc aattaaggac
    37921 tcgataaatt cacgcaactc atcgccgaga tcaaccgtca tcgttctggc cattttacgc
    37981 ccgcctgtaa gtaaaaatct tacatcagtg tatgtcataa accggatagc gtcacgaaag
    38041 tattagaaga tattttggca catgaacatg ttcaggagga taagtatggg atggtaaaca
    38101 aagaaagcac aacgagtgct cgtcagtgcc cactatttta atggcggcgg ttactcttaa
    38161 ttattaataa ctaagcgtta tttggatata aagccggacg ccaacaaccc acgcccggca
    38221 tcgtaattac gtattgtctt tcaccgtctg gcttgtgcca ttggctttaa cggaggcaat
    38281 tccggtttca cgagattgcg cggtggcgta catctggctg gagccgataa tttgatgatt
    38341 agcggctttc agattgaaat agaatttgcc gttacttgcc gtttttttct catagcgttc
    38401 ttcttgcggg ctgttgctac gcaccgacgc gatgcccttt tccgcagagg cttttgaggt
    38461 atacagctcg ctggtgagga tagtctcacc attgcccgct tttagcacaa accggaactg
    38521 attatcactg ctcttactga gttcaaacca accagccata atgactcctt gattgaaatt
    38581 gcgtatcgaa aacagcatct aaaggtgctg tttaccgatt aaagtatgag cgattttctg
    38641 atgcctgcca gggcggagag ataatgtcag tttgctgacc atgcgtggga taatgttgcc
    38701 ggatctggtc tgaacgccat ctccggctaa caaagtcatg tcgctaaaac taaacgatgc
    38761 ggcaggcgtc ggcttcccag cggtaaccaa cgccgtaaac ggcgcggata aatgactgtt
    38821 cggcgtcgag agattccagc ttgcggcgca ggtttttaat gtggctgtcg atggtgcggt
    38881 cggttactac gcggtagtcg tcataaagat gattgagcaa atgctcgcgg gagaacactt
    38941 ttcctggttc atgagagagc gttttcagca gacgaaattc cgcaggcgtc aggtcaagca
    39001 ttttaccgcg ccatgaagcc tgaaaacgac cttcgtcgat aatcaacggg ctttcagcat
    39061 cctgttgctg caactcgcgc tgcggtttgc aacggcgcaa aatggttttg acgcgcgcta
    39121 ccacttcccg tgggctgtac ggcttacaga tataatcatc tgcgccaatc tccagcccca
    39181 gcaggcgatc gatctcttcg atttttgccg tcaccatcac gatcggaatg tcagaaaaac
    39241 gacgaatttc ccggcacagc gtcaggccat cggtgccagg gagcatcaga tctaacagga
    39301 tcagatccgg tggtgtctgg cgcacatacg gcagtacctg atcgccgtgg ctgataagcg
    39361 tcggcgcata gctcgcagca cgcagataat caatgagcaa ctgccccagc ttcggttcat
    39421 cttccacgat caaaatacgc ggtgtgtttt cgtcgattgg taactcggtc atacttctct
    39481 ctgtaaatcc cgttccagcg gtaactctac tgtaatgctt accccgccaa aaggcgaatg
    39541 ggcagcaata atgcggccat tatgtgcttc aacaatgttc aggcaaatcg ccagccccag
    39601 cccggaacca ccgctggcgc ggttgcggga accttcggtg cgataaaaac gttcaaataa
    39661 tttttgtagc tgctcgtcac tgacacctgg cgcactgtcg gcaaaggtca ggcgcaccgt
    39721 tttgtcgtgc tgctcggcag agattttcag gctgccgccg ctatcagtgt agcgcaggct
    39781 gttttcgagt aagttattga ataactgcat taaacggtcg cgatcgccaa atacggtaat
    39841 actgtctggc agggaaaact gcagtttcag gccacggctg gcaaatcgtt cgcgaaatgc
    39901 gccgcccgcc acttccagca gtgggatcaa atctaccggt gctttttgat aggcgagagc
    39961 gccttcatca gacatcgaca actgatgaag atcgtcaacc agtttagtca gtgtaccgac
    40021 ttccgcctgt aaagacgcca ccgtctccgg cgtgaatttg cgcacgccat cttgaatggc
    40081 ttccagttca ccgcgcagca ccgctaatgg cgtacgcagt tcgtgtgaaa tatcggccat
    40141 aaaatcgcgc cgcatttgct ggtttttctc cagcgtgctg gcaagctggt tgaagtcttg
    40201 cgccagtttg cccagttcat cttcactggt gggcgttacg cgggtagtga aatcgcccgc
    40261 cgccagtttg tgcgtgccat cgacaagtcg ttttaccggt gccagcaaac cgcgcgccag
    40321 cagaaaggtg gcgagcgccg cgagtaacgt tgccagggcg acgatcagcc agctggtttg
    40381 ccgctgttgt ttatcgaaat tgatatcagt attgcgcgtt aaccgctcaa cgggggaggc
    40441 gatcaccgcg ccaacttccg caccgttgac cagaatgggt cgccgtgtac cgtcaggtgg
    40501 aatcggcgct cgcggaccaa ccagcacttt gttgttttga tcaaccaccc agaactgggt
    40561 acgccagccg tgcggtggca tacccgggcc gggtttatct tccgaattat cgtgttcaaa
    40621 tgaacgcagg atctgaaaga caaagcgatc attgttgcgc aggaagcgcc agttgccatg
    40681 ctgcgcatac tgctcgccaa gcgcatcact taacagttgt aatcgttgtt cattaccatg
    40741 cttgatgtaa tcaataaagc cacgctcaaa actgatacgc accgcccagt gcatactgat
    40801 cagcaagaca atgcaggtgg cgaaaatcgc cagaaacagt ttgccggtaa taccgggtcg
    40861 ccagaacttc attgcgcgct cctttttcgc cgcgaaatag ctacattttg atgcgtatcg
    40921 ttcggcactc tggcaaagat gaacgccgga agggcgatga tcaacgccat gctaagccag
    40981 gtgtacataa agacggtttg tgtggtgccg ctgtcgacgc tgacatgctg cgaaccaaaa
    41041 agtcccagca acagcccggc gatggtgacg ccgatactca tcgacaattg cataatcatc
    41101 gacagcaggc tgtttccgct gctcgccaga ttgtccggga gatctttcag cgtcagggtg
    41161 ttcatggagg agaaacgcgt tgagttgacc attccttgta aaaacaggac gaacggcaaa
    41221 acgtagtacc agcccaacag agcggtagtc ataaacaaca gggtgaccag cgacagaccc
    41281 agcgtggtcg ctaccagtac ccgacgataa ccaaagcgat tcaccacctg aaccacaatt
    41341 cgcttcattc ccatgctgcc aagcaccatc gggatcatca tcagtccggc atgaaacggc
    41401 gagaaaccga ggccaatttg caggaaaacc ggtgtcataa agggcaacat gccactgcca
    41461 atacgtccgg caaagctccc cgccaggccc agcgaaaagg tacgagtgcg gaacagtttc
    41521 agactgaaca gggcgcggtt gttatttctg gcgtgcagca gataaagcac cagtgccacc
    41581 acgccaactg cgaccaggcc agcaatcgcc agcggcgata aacctgtacc tttactgccg
    41641 tccagcgcca gggttaatac cgccatgcca accgccagca ataaaaagcc ggaaagatca
    41701 aagcgccgcg tctgcatggt gtagttcggc attaacatca atgtggcgat cgcaccgata
    41761 atccccaccg gaatgttgat caaaaagatc cagtgccacg acgcgtactc caccagcaga
    41821 ccgccgagcg ccggaccgag cagcggaccg acctgaccgg gtaacgtgac aaaggtcatc
    41881 gccgccatat attgctcgcg cggtacgatt ttcatcaccg tcaatctgcc gaccggcacc
    41941 atcatcgcgc cgccaacgcc ctgtaacgcg cgtgccagca acagttcgtt cagcgtgccg
    42001 gaaagcgcgc aaaacagtga accgagagta aacagcacga tggcggtaaa gaaaatattg
    42061 cgcacgccga ctttgtccgc cagccagccg ctggcgggca gcatcaccgc cacggtcagc
    42121 acataagaga caatgaccat gtgcatatgc aacggacttt ccccgaggct ttgcgccatt
    42181 gagggaaggg cggtgtttac gatggtggtg tccagcgact gcataaagaa gccgaaagcc
    42241 acaatccaca attgccaacg ggtgctgtcg ggaagatctg tcatttactc ggttaccgtt
    42301 tgtttaggtt tacgcgaaaa acgcagccgc agacggtcga aaaagagata caccaccggc
    42361 gtggtataca gcgtaaggag ctggctcatt accagtccgc cgacaatggt gatccccagg
    42421 ggttgccgca gctccgagcc gtcgccgccc gacaacacca gcggcagcgc gccaaacagc
    42481 gccgccaggg tagtcatcat aatcgggcga aaacgcagca gacaggcctg gaaaatagct
    42541 tcctgcggcg tcaggttacc gtgccgttgg gcttcaagcg caaaatcgac catcataatg
    42601 gcgtttttct tcacgatgcc gattaatagc atgatcccta tcagggcgat taggctgaac
    42661 ggggcattga acagctccag cgccagcagc gctccaacac ccgccgaggg cagggtggag
    42721 agaatcgtca gcgggtgcac ataactctcg taaaggatac ccagcacgat atacaccgtg
    42781 gcgatggcgg cgataatcag gatcacctgt gagttcatcg tctcctggaa cacctgcgct
    42841 gttccggcaa aactgccgcg caccgtcgaa ggcacaccaa gctgggtcat tgcgcgatcg
    42901 atcgccgcac tggcgtccga gagcgatttt ccggtcggca ggttgaacga aatggtcgag
    42961 gccgccgata atccctgatg attcaccgat agtggggcat tcgccggttg ccatttagcg
    43021 aaatacgaca gcgggatcgc tttgccttcg ttattgataa cgaacatttt ttccagcgca
    43081 ctgatgtcct gggtatagcg cggatccact tccatcacca ctttatactg gttcatcggc
    43141 tggtaaatgg tcgagatttg ccgctgaccg aaggcgttat ttaacagact gttggcggct
    43201 tgtacgtcga ttcccagccg tgccatggtg tcacggtcgt aaaccagatt catctccgcg
    43261 ccgttatcct gctgatcgga gttcacgtcc gccagttccg gcaacgtcgc cagttttttg
    43321 cggattttcg gctcccattc gcgcagtgcc gccaggtcgt cggataacaa cgtgtactgg
    43381 tagctggcgt tcgactgacg cccaccaacg cgaatatcct gtaccgccat caggaacaga
    43441 ttcgcccccg gttctttcgc cagttttacg cgcagacggt cgataatttg ctgcgccgtt
    43501 tcgctgcgtt cgtcgcgtgg cttgagggtg ataaacatca tcccactgtt aactcgtgaa
    43561 ccgccggtaa agccggtgac attatccact gccggatcgt cacggataat tttcatgaaa
    43621 tcctgcaatt tgccgcgcat cgcctgaaac gaaatgctct gatccgcctg tatcccgccc
    43681 atcagcacgc cggtgtcctg ctccgggaag aaggttttcg ggatcgagat atacagccag
    43741 atattcagcg caatggtgcc aagcagcacc acacccacca gacgggtatg attgagcacc
    43801 catttcagcg acttgccgta gccttgttgc agggctacca acatgcggcc aaaaccacgc
    43861 agtcgctttt gctcacgcgg cttgctggct ttcagcatcc agccacacat cattggcgtt
    43921 aatgtcagag aaaccagcaa cgatataaca atggcgacag aaagcgtcac ggcaaattcg
    43981 cgtaacagtc ggcccggcaa tccgcccatc aacagcagcg gcaggaacac cgccaccagt
    44041 gacagactca tcgatagcac cgtaaagccg acttcgcgag taccttgtag cgcggcctgt
    44101 agcggtttca tccccgcttc cagatgacgg gcaatgtttt ccagcaccac gatggcgtca
    44161 tccaccacga aaccagtagc gatggtgagc gccattaacg aaaggttatt gagactgaat
    44221 ccgcacaggt acatcgccgc aaacgtacca atcagcgaaa ccggcaccgc aacggcggga
    44281 ataatagtgg cgcgacccga gcgcaggaat aaaaacacca ccagaatcac cagcgccacc
    44341 gagataatca gcgtttgctc gacttcttcc agcgaggcgc gaatggtggg ggagcgatcc
    44401 tgagcaattt gcagatcaat cgccgccgga atggtttcct gcaactccgg taattttgcc
    44461 cggatgctgt ctaccgtctg gataatattg gcttccggca gcttgcggat catcagcaaa
    44521 atagccggtt tggcgttggt catcccggcg ttgcgcacat cctgcaccga gtcggtcacc
    44581 gtcgccacat cgcccagacg aaccgcgccg ccgttgttgt aatgaataat caacggctga
    44641 tattcggcgg cggtttttag ctcatcattg gtctggatct gccagcggtg agtgccatct
    44701 tccagcgccc cctgcggttt acgcacgttg gcattgctga tggcggtgcg tacgtcgtcc
    44761 agcgagacgc cctgattaaa cagcgcctgc ggattcagcc cgacgcgtac ggcgggcagc
    44821 gagctgccgc caacatcgac atcaccgaca ccgtcgattt gcgaaatcgt cggagccagc
    44881 tgcgtcgagg caaaatcgta cagttcaccc tgcgaataag tatcggatgt cagcgtgagg
    44941 atcataattg gcgcatccga cgggttagct ttgcgatagg tcggacggct gggcatcccg
    45001 ctgggcagca aactttgtgc agcgttgatc gccgcctgca catcgcgcgc cgcgccgttg
    45061 atatcccggt caaaatcaaa ctgcaaaata atacgcgtgc tgccgagcga actgctggag
    45121 gtcatttcac tgactccggc aatgcgccca agtgagcgct ccagcggcgt ggcaacggaa
    45181 gacgccattg tttctggtga cgcaccgggc agcgaggcgc tgaccataat caccggaaaa
    45241 tcgacctgcg gcagcggggc gaccggcagc atacggaagc ccagtatgcc gcacagggta
    45301 atggcgaccg acagtaaaat cgtcgccacc gggcggtaaa tgaagagggc aaaaaacttc
    45361 acttacgcct cctcttcatg gcgagcaaag cgacttttgg tccacaatgc caggcggtcg
    45421 aacagcagat aaatcaccgg cgtggtgaac agcgtcagca cctggctgac aatcagaccg
    45481 ccgaccatgc cgatacctaa cggacgacgc agttccgcgc cgaccccggt actcaacatc
    45541 agcggcagcg cgccaagcag agccgccagg gtggtcatca ggatcggacg aaaacgcaac
    45601 agacaagcct ggtagattgc atcgcgcggc gacatgcctt gctcgcgctc agcagccagc
    45661 gcgaagtcga tcatcatgat ggcgttcttc ttcacaatac cgatcagcaa aataatgccg
    45721 ataatcgcaa tcacatccag ttcgctaccg gcaatcatta atgccagcag cgcgccgacc
    45781 cctgcggtgg gtagcgtcga gagaatggtg atcgggtgaa taaagctctc gtacagaatg
    45841 ccgagcacga tatacatcgc caccaccgcc gcgacaatca gccagacagt gctgcccagc
    45901 gccgactgga aggcgagggt gctgccctga aattgcgtgg tgatatccac cggcagattc
    45961 agcgtctttt cggtgtccat aatcgcctgc accgcatcgc ccagcgaata gttatccggc
    46021 acgttaaagg agatggtcgt taccgggaac tgatccagat ggttgatgga aagcggcgca
    46081 aaacgctgtt caactttggc aattgagctt aacggcacca cgccgccgtc gctactggtc
    46141 aggcgaatgg tatccagcgc cgcgaggccc ggggtaattt cggtgttgtg ctccagcact
    46201 acgcgatact ggttagcctg agtataaata gtggaaatca gtcgctgacc aaacgcgttg
    46261 tacagggcgt tatcgacatc cgccatgctg atccccagac ggctggcgct gtcgcgatca
    46321 acattgacat acgccaccag ccctttgtcc tgccagtcgc tggagacatc agaaagctgt
    46381 ggcagttgct ggagtttttc catcaactgt ggcacccagg tactgagcgc atccagtgag
    46441 gtggcctgca aggtaaactg gtactgggtg cggctgacct gagtatcaat agtcagatcc
    46501 tgcgttggtt gcaggaagag atcgacgccc ggcactttat ccaccgccgt ttgcagacgg
    46561 gcaatgactt tttgcactcg gtcatcacgt tcatccagcg gtttgaggtt gatttgtaaa
    46621 cgtgcactat tcagcgacgg gttagtgcca tcaacgccaa caaatgaggt caggctttgc
    46681 actgccggat cctgcaaaat cacgtccgcg acctggcgtt gtcgctgggc catattggca
    46741 aaggagctgg attgcggtgc ctgcaaagtg ccctgaataa tgccgttgtc ctgtaccggg
    46801 aagaaacctt tcggaatgaa cacccataag agaacgctaa gcagcagcgt gctgagcgcc
    46861 acgcttaagg tcagccacgg atgattcagc accttcgcca gtccacgacc ataggcggcg
    46921 attatcctgt cgaacatttt ttccgaggca cgggagaagc ggttctgttt acgcaacgac
    46981 tcctggctga gcatccgcgc gcacatcatc ggtgtcaggg tcagcgacac caccgctgag
    47041 atcaaaatcg ctaccgccag cgtaatagca aattcgcgga acagtcgccc gacgatatcg
    47101 cccataaaca gcagtgggat caacaccgca atcagtgaga aggtcagcga gataatggta
    47161 aagccgattt cacctgcgcc cttgagcgcc gccgccaacg gtttttcgcc tttttcgata
    47221 tagcgggaaa tgttttcgat caccacgatg gcgtcatcga ccacgaatcc ggtggcgata
    47281 gttaacgcca tcagtgtcag gttattgatt gaaaaatcga gaaacaccat aaccgcgaaa
    47341 gtgccgatta acgacagcgg tacagcaaca ccgggaatga tggtcgccgg aatattgcgc
    47401 aaaaacaggt agataatcat gactaccagc gcgatagcca tcatcaattc aaactgagta
    47461 tcatcgacgg atgcgcggat attggtggtg cgatcggaaa gcactgtcac cttcaccgat
    47521 ttcggcagac tctcagtgag ctgtggcagc atctgccgaa tgctgtcggc ggtggagata
    47581 atgttagcac cgggctggcg ctgaacattc atcacaatgg cctgttcttt gttcgcccac
    47641 gcgccgagcc agctgttttc tgcaccttgc tctacagttg cgacatcgcc cagacgaatt
    47701 ggcgcgccgt tctggtaggc gatgattagc tggcgatact cttcggcgga ttgcatctgg
    47761 tcgttcgcgg aaagcgtgac cgcacgggaa gggccgtcga ggctaccttt tgccgagtta
    47821 acgttagcgc cggtaatggc ggtgcgcacg gtttcgctgg tcaggccgag ggcggcaatc
    47881 gcctgagcgt taagtttgac gcgaacagcc ggacgctgac cgccggaaag cgtcaccagg
    47941 ccgacgccgg aaatctgcga gattttctgc gcgacgcggg tttccaccat atcttccact
    48001 tgcgtcatcg gcatggcggt tgaggtgacg gcgagcgtca tgatcggcgg atctgccggg
    48061 ttcactttgc tgtaaaccgg cgggttaggc agatcgctcg gcaacaagtt ggtcgcagcg
    48121 ttaatcgcgg cctgcacttc ctgctcggcg acatcgagcg gtaatgttag ctggaactgc
    48181 aaagtgataa ctgacgcacc gccggaactt tgcgacgaca tctgtttcag gccagacatc
    48241 tgcccgaact ggcgttcgag cggcgcggta acggcagagg tcatgacatc cgggctggca
    48301 cctgggtaga gcgtgaccac ctgaatggtc ggatagtcca cttccggcag cgccgaaacg
    48361 ggcagggcgc gataaccgat aatcccggcg agtaagatcg ccaccatcag cagcgtggtg
    48421 gagacaggac gcataataaa caggcgcgac gggccgcctg tgctgctcgg gggtaacacc
    48481 tgcatcagga gcgtgctcct tttttcgcgt attcgcggct ggtggctttc tcttccggag
    48541 tggtggcgct ctgggcttcc accacttcca ctttcgcccc ttcggtcagg cgatcaatgc
    48601 cgtctgtcac cacgcgatcg cccgcagaaa tacctgcacg gatcaccact ttctgactgt
    48661 cctgaatgcc cggcgtcacc agatgtttgc tgaccttgtt ttcgctattc agcacccaga
    48721 caaaatggcc ttcattgccc atttgcaggg cggctgttgg gatcactacg gcgttttgtt
    48781 cggtgtcgac taacatgcgc gcgttaacaa actgattggg aaacagcgca tcatcctgat
    48841 tattaaagcg tgctttcact ttaatcgtac cggttgtggc atcgatttgg ttatcgaggc
    48901 ttaacagcgt gccttcactt aatttcttcg aattggtgcg atcccaggct tctaccacca
    48961 gcggttttcc ggctttttgc gcctgcacta cagtagcgat atcgctttcc ggcagggtaa
    49021 agactaaatc gataggatgc gtctgggtga tcaccacaat tccggtggta tcaccactgg
    49081 agatttggtt accaacatca acctgcttga gaccaacgcg accatcgact ggtgcggtga
    49141 tgcggctcca gtcgagttgc aactgcgcgc tggcgacgct tgcttcatca gccttaatgg
    49201 tgccttcggt ttcactgacc agcgcctgtt gggcatccag ctcctgacgg gagacgagat
    49261 tggtttttgc cagttgctga taacgcgcca gatcacggcg ggcgttagca agcgtggctt
    49321 tatcttttgc cagttggccc tgggtttgtg ctaatgcaac tttgaactgg ctggggtcaa
    49381 tttctgccag taaatcgcct gctttgacct gctggccttc ctggaaatgt aacgccatca
    49441 gttgaccgtc cacgcggctg cgcaccgtaa cagtattagc ggcgataatg gtgccaagcc
    49501 cggtgaggta acgcggaact gcctgttcta cggcggtcgc cgcctgaacc ggggctaatg
    49561 ggccggaacg cataccacgg cgaccacccg ctggcgattg ctgcgcttgt ttcgtcgccc
    49621 ctggggctgc actccgggag tcattgcggc cttgccagaa ccagaatgcg gcgatggcgg
    49681 cgataaccac cacgattacg attacccaac gggatttata actgcctttc atcgttaaga
    49741 gtttctcttc ctgaaacgtt tcgcggaatg atactagtgt agccagccaa taagggagaa
    49801 aaatggagga attatgaaac actatcggga atcgtctgaa tggcgggcac atttgcgagc
    49861 acgcatccag taataacaca ggaaactatt ttatctacgc gttagcgata gactgcatac
    49921 agggcgaaag gaggtaagcc gcagatttca gccggatgct gaaacgggaa agcccctccc
    49981 gaggaagggg ccttaaataa ggaaagggtc atgatgaagc tactcatcat cgtggtgctc
    50041 ttagtgatta gctacccagc atactaagac taccagggcg ggggaaaccc cgctctaccc
    50101 tcactcctga aagtatgcct tcacgataag attgtcaatc cgcaggcttt gccgtctgcg
    50161 atcctgccag caaaaattct ttgcgagacg ttacgcaata atcacactgg aaactatttt
    50221 atctgcaagt cagcgataga ctaagtgcat ggcgaaagga ggtaagccga cgatttcagc
    50281 gggacgctga aacgggaaag cccctcccga ggaaggggcc ttaaataagg aaagggtcat
    50341 gatgaagcta ctcatcatcg tggtgctctt agtcataagc ttccccgctt actaagacta
    50401 ccagggcggg ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acgataagat
    50461 tgtcaatccg caggctttgt agtctgcgat cctgccagca aatattcttt gcgagtcgtt
    50521 acgcaataat cacagaggaa actattttat tcacgcgtta gcgatagact gcattcaggg
    50581 cgaaaggagg taagccgatg atttcagcgg gacgctgaaa cgggaaagcc tctcccggag
    50641 aagagggctt ttaataagga aagggttatg atgaagcacg tcatcatact ggtgatactc
    50701 ttagtgatta gcttccaggc ttactaagaa caccaggggg agggggaaac ctctccctaa
    50761 ccctcactcc tgaaattatg tcactccgcc cttagtgtca atcgacggct ctccgttctt
    50821 gcgaggcgaa tagcaaaatt ttctgcgccg ccgggcctaa ttctgaaagt ttcttcgttt
    50881 gctcttattt cattattgca gtcggtatga taccggacct cgataattct ctaagaaagg
    50941 atttcttatg agcgaacaaa tcacgtttgc caccagcgat tttgccagta acccggaacc
    51001 acgttgcccc tgcattttgc tgctggatgt ttctggctcg atgagcggca gacctattaa
    51061 tgaacttaac gccggattgg tgacctttcg tgatgaactg cttgccgatc cgctggcttt
    51121 aaaaagagtg gaactcggca ttgtgacgtt cggcccggtg catgtggaac aaccctttac
    51181 cagcgccgct aatttttttc cgcccatcct gtttgcccag ggcgatacac caatgggtgc
    51241 ggccattacc aaagccctgg atatggtaga ggagcggaaa cgtgagtatc gtgcaaatgg
    51301 tatttcgtat tatcgcccgt ggattttcct gattaccgat ggtgcaccaa ccgatgagtg
    51361 gcaggctgca gccaacaaag tgtttcgggg ggaagaagat aaaaggtttg ctttcttttc
    51421 catcggtgtt cagggggcgg atatgaagac tctggcacaa atcagcgttc gtcagccttt
    51481 gcctctgcaa ggattacaat tccgtgaact gtttagctgg ttatccagtt cgctgcgttc
    51541 ggtttcccgc tccacgccgg gaacggaagt tgtgctggaa gcgccaaaag gctggacgtc
    51601 agtgtgagct ggcgtctggt ttatgcatcc gctgttggaa catcgcacat cagcgcggac
    51661 ttgccttgcc aggatgcctg ccagatgcag gttgcctggc taaatgacca gcagccatta
    51721 ctggtgatgt tccttgctga tggcgcaggt agcgtttcgc agggaggaga aggcgcgacg
    51781 ctcgccgtca atgaagcgat ggcttatatg tcgcaaaaag tgcagggtgg ggaattgggg
    51841 cttaatgata tcctcgccac ggatattgtg ctgaccgttc gtcagcgact ttttgccgag
    51901 gcggaagcca aagaattagc agttcgcgat tttgcctgca cgtttctggg attgatttca
    51961 tcggctaatg gcaccttgat tatgcagatt ggcgatggcg gcgtggtggt ggattttggt
    52021 cacggtctgc aattgccgct tacgccgatg gtgggtgaat atgccaatat gacgcacttc
    52081 attaccgatg aagatgccgt ttccagactg gaaactttca ccagcactga gcgtgtgcat
    52141 aaagttgcag catttacgga tggtatccag cgactggcgt taaatatgct ggataattct
    52201 cctcatgtac ctttctttac cccgtttttc aatggactgg cgtcagcaac gcaggaacaa
    52261 ctcgatttat tacctgaatt gttaaagcag tttttgtcca gcccggcggt aaacgagcgt
    52321 acggatgatg ataaaacgtt ggcactggcg ctgtggttac catgaaaccc actttatata
    52381 ctgctactgg tgagtgcgtt acgccaggcc gtgaactggg caaaggcggt gaaggcgcgg
    52441 tttatgatat caatgagttt gtcgatagcg tcgccaagat ttatcacacg ccgccacccg
    52501 ccttaaaaca ggacaaactt gcctttatgg ctgcgacagc tgacgcgcaa ttgttgaatt
    52561 atgtcgcctg gccgcaggca acgcttcacg gtggacgagg cggaaaagtt atcggtttta
    52621 tgatgccaaa agtttctggt aaagaaccga ttcatatgat ctatagcccg gcacatcgtc
    52681 gtcaacgtta ccctcattgt gcgtgggatt ttctactcta tgttgcgcgc aatattgctt
    52741 catcttttgc tacggttcac gagcacgggc acgtcgtggg tgacgtaaac cagaacagtt
    52801 ttatggtagg tcgcgacagc aaagtggtac tgattgatag cgactccttt cagattaacg
    52861 ccaatggcac actgcattta tgcgaagtcg gcgtgtcgca ttttacgccg ccagagctac
    52921 aaaccttgtc gtcatttgtc ggctttgaac gcacagaaaa tcacgataat tttggccttg
    52981 cgttgctgat ttttcacgtc ttgtttggtg gccggcatcc ttattctggt gtgccgctta
    53041 tctctgatgc gggtaatgcg ctggagacgg atattaccca tttccgttat gcctacgcgt
    53101 cagataatca gcgacgtggt ttaaaaccgc cgccacgatc gattccgcta tcgatgttac
    53161 cgggcgatgt tgaagccatg tttcagcagg cattcacgga aagtggcgtg gcaaccgggc
    53221 gtccgacggc taaagcgtgg gtagcggcac ttgattctct gcgccaacag ttaaagaaat
    53281 gtcccgtttc ggcaatgcat gtttatcccg ctcatttgac cgactgcccg tggtgagcgc
    53341 tggataatca aggcgttatc tattttattg atctcggcga agaggtcatt accaccggcg
    53401 gtgattttgt gctggcgaaa gtctgggcga tggtgatggc gtcagtagca ccgccagcat
    53461 tgcaattgcc attacccgat catttccaac cgactggcag gccgcttcct ttaggcctgt
    53521 tacggcgcga atacatcatt ctgattgaga tcgcactgtc agcgttatcg ctgttgcttt
    53581 gcggccttca ggcagaaccg cgttatatta ttttggttcc tgtgctgtcg gctatctgga
    53641 ttattggcag tctgacaagc aaagcgtaca aagcagaaat ccagcaacgc cgtgaggcat
    53701 ttaatcgcgc gaaaatggac tatgagcatt tagtcagcca gatccaacag ttgggcgggc
    53761 tggaaggttt tatcgccaaa cgggcgatgc tcgaaaaaat gaaggacgaa attctcgggt
    53821 taccggaaga agagaaacgc gctctggcag cacttcatga cactgcaagg gaacggcaga
    53881 agcagaagtt tctggaggga ttttttattg atgttgcctc tattcccggc gttggccctg
    53941 cgcgtaaagc ggcgttacgg tcttttggta ttgaaacagc cgcagatgtt acccgtcgtg
    54001 gcgttaagca agtgaaaggg tttggcgacc atctgaccca ggcggttatc gactggaaag
    54061 cgagctgtga acgccgtttt gtgttcaggc cgaatgaagc gataacgcca gcagacagac
    54121 aagcggtaat ggcgaaaatg accgccaaac gacatcggct ggaatcgacg ttgactgtcg
    54181 gcgcgacaga gttgcagcga ttccgccttc atgctccagc acggaccatg ccgttgatgg
    54241 aacctttacg tcaggcggca gaaaaactgg ctcaggcgta ggcagattta agccgctgct
    54301 gagccatttt cttttaacga aacaccactt ccgcccagcg tgccagcccg gcggtgacgg
    54361 agccaaagtc atcgccgcct gcaatcggaa ttcccggcaa ctgttctgcc agcgcttttt
    54421 taatcagcgg cgaacgggcg ctaccgccgg tcaggtagat aacgtccggt ttttcctggg
    54481 cgttatccag ggccagttgc acctgttcca gaatccgcgc cagtggctgg ctgagggcgc
    54541 tttccagccc ttgctggcta atcagcgtag ccagttcatc gctgataaac ggcagtgagg
    54601 cgcgggtttc cgctacgctt gaaagagcaa ttttgcactc ttctgcgcta cgcaccaggc
    54661 gatagcttaa acgctcacgc cagactttct gtagcagggc cactttttcc ggttcgcggg
    54721 catcgcgtac cagatcgtta agcagacgac cgttggcact actgtagaaa tcactctgcg
    54781 caggtacgtc gttgatggca accgcattcc accacggcag aatcggcagg gcgatgcctt
    54841 tttcggtttc gccacccatg cccagtaacg gcatcaggtt tttaaacgcc agtgcgatgt
    54901 ccagatcgtt accgccaata cggcaaccac tgtgacccag caggctggct tcacgatcga
    54961 gacgcgaacg ccactgcggc cccatcagca gcaatgaaca gtcagtcgta ccaccgccga
    55021 tatccaccac cagcacccgt ttttcttcct gcaaggtggc ttcgtaatcc agcccagccg
    55081 cgaccggctc gtactggaat accacgtccc tgaatccggc acgcttcgcc gcgcgttcca
    55141 gaatcccttg cgcctgggcg tttgcttcat cgccgcccag cccctggaag ttgatcgggc
    55201 gaccaatcac cgcctgagta atcgcttctg gcagctgcgc ctgcgcctgc tggcgaatgt
    55261 gcaacatcat tgcgcagacc agatcctcaa acagcgctac ctgctgcggt tttaagccgc
    55321 tggcaccgag gaacgatttt ggtgatttca caaaccacac ttcttctgga tcatcaatgt
    55381 actgtgccag tgaggaaaga ccgaactgca cgcttttcgc cgtaacatcg atatcttctt
    55441 cgcggttata acgaatcgcc cgacgtaaca gcgcctgcgt ttcatcgtcg tctgccggaa
    55501 catcatgatg gcggtacagc cattcgctta ccgattcacg cgttggcgcg caaagcattg
    55561 aaggcagcag cgtgctgtcg ttttccattt tcagtaattg cggtttaccg tcacgcatga
    55621 ccgccactga acagtttgct gtaccgtaat caaaaccaat aaacacgaat taatccccat
    55681 gccggtgaag aaggggcgtg actttagcga aatgttgctg tcgcgacaac cggaatatga
    55741 aagcaaagcg tagcgtctga ataacgttta tgctgaaagc ggatgaataa ggagatgcga
    55801 tgtataccct gaactggcag ccgccgtatg actggtcgtg gatgttggga tttctcgccg
    55861 cccgtgcagt gagtggtgtg gaaacggtcg ctgaggatta ttatgcccgt agtctggcgg
    55921 tgggcgaata tcgcggcgtg gtgactgcta ttccggatat agcccgccat actctgcaca
    55981 taaatttaag tgcaggttta gaacccgttg ccgctgagtg tctggcgaaa atgagccgcc
    56041 tgtttgatct gcaatgtaac ccgcagattg ttaacggtgc gttgggcaag ttaggcgcgg
    56101 cgcggcccgg attgcgttta cccggctgta ttgatgcttt tgagcagggt gtgcgggcga
    56161 ttttaggcca actggtgagc gtggcgatgg cggcaaaatt gactgccaaa gttgtacagc
    56221 tttatggcga acggctggat gattttccgg agtatatctg cttccccacg cctcagcggc
    56281 tggcagcagc cgacccgcag gcattaaaag cgttaggcat gccgttgaaa cgggcagagg
    56341 cactgattca tctggcaaat gcggcgctgg agggcagctt accaatgaca attccgggcg
    56401 atgtggagca ggcgatgaaa acgctgcaaa cttttccggg tatcgggcgc tggacggcga
    56461 attatttcgc tttgcgtggc tggcaggcga aagatgtttt tctgccggat gattatctga
    56521 ttaaacagcg atttccgggg atgacaccgg cacaaatccg ccgttatgcc gagcgctgga
    56581 agccctggcg ttcttatgcg ctgttgcata tctggtatac ggaaggctgg caaccagacg
    56641 gaacggacga attgtaggcc tgatgcgtcg catcaggcaa tgggcaaacg ctccctcagt
    56701 taatcgcgaa ataactgcta ttcaccagca aatccagcgg ttgggcatcg gcaatcacat
    56761 caccataaat cagatcgacg ccgatgccag aaagcgtatc catcactaag ggtaaaacga
    56821 ctggcccggc gatggttttc atcccgagtc gctgagcgtg cccctgaata atcgtaatca
    56881 gcatctcatc catcaaatta ccctgcacgt tggcgcataa ctcaccatca agtagcaggt
    56941 aatctgccat attcgctttc agcgagttga agatttgcag atcgcggccc acctggctga
    57001 gcactatccg acatcccgcc agccgcagtt tttgcacgct ttctgcgtga tctaaaatcg
    57061 cgtctgccgg aataatcaga tgtaataacc gtggtggtag agggctattt tccagctgct
    57121 caagcagatc attcaccagc gtggcgctac tcaaaccggc aacggaaagg gggagggcga
    57181 tgcttatgcc tttactggca accgcttttg cggccagctg gaaaaattcg tggaataccc
    57241 gtcggtcaag agcatggcta agtgccggat cgctgaagct acgacgaaat ttttgttcat
    57301 caataatctc gccttcgcaa ctccagagtt taagcgaaat cagccacaaa ttacgcgctt
    57361 ccgggatccg cggcgaagcg acaccgtggg ccatcatcat caactgattc tctttaatca
    57421 tccgccactg ttcatcaagc gacatcgccg cccgttcgct atgtgcggca gcttgctgtg
    57481 gttcgtaaac cgtcacccgg ccccgtccac catttttgga ggcataacag gcgatatcag
    57541 cctgcgacat cacttctgcc gcctgatgat tgttgtcatc aatcaaggta atcccggcac
    57601 tggcacttac ccgatgcaca cggccttccc atataaagtg atagtcattc acggcactga
    57661 taatgcgtgt agcgataaaa cgcgcgcttt caacattaca gtctggtagc agcagaccaa
    57721 attcatcccc accgagtcgc gccagcacgt cgctggaacg cagcatactc agcatcaacg
    57781 acgccagttc gcgcagcaaa gcgtcgcccg ccgcatgccc ggcgctgtca ttcaccgctt
    57841 taaagcgatc aagatcgata aacaccaggg catgtcgctg atgtgtactg tttaccgttt
    57901 gcagcagcat acgcagttgc ttctcaaaac tggcgcgatt ggcgagatgc gtcagtgcat
    57961 catgggaggc gctgtagctc agctggcgca gcattttgcg tgattcggta acgtcctgaa
    58021 tcaccagaac cgaaccaata ttgctgccgt ccagagtact taacggcgta atactgtaat
    58081 gcacgtcgta gctgccgccg ctccggcagt gcaacaccac atcttgttca atcgcggaac
    58141 gtgaggtgtc ggcactgtaa atgttctcca tcaggggacc attgtcgcca aaggtaatat
    58201 gcaacaccgt caggagcgga acacctaatg cttcttcctg cgtccagccg ctcatcttct
    58261 ccgccactgg attcataaag gtaattttca ttgccatatc aatacagacc acggcttcgc
    58321 cgatggagtc cagcgttatg tgcaggcgct ctttttcctg aaacaatgcc tcgttaagct
    58381 gtttaacctc agtcatatcc atattaatgc cgagcaggcg ttcgacttcg ccttctttat
    58441 tcagtacccg gttggcgagg gcgcggatat ggcgaatacc gtcttttacg gtaatgcgaa
    58501 attccagttt aaagggcgag cgtgattgca acgaatcact ttttcggcat gctggcgatc
    58561 ttccggcagc acgcactcgt accacacctg ccagttcggt ttgatatgtg gaggaatttc
    58621 atacagctcg aacatccgct tatcccagct aaaaatattc ggcttcaact cccactccca
    58681 gatgccaatc ccgcccgctt cgttagccag cgtgatgcgc tccatcagtt gctgattcac
    58741 ctgttcggtg cgttttagct cgttaatgtc ttcaatctga gcgataaaat agagcggcgt
    58801 gccatccgtg tggcgcacca gtgagacggc aagcaacgcc cagacaacat cgccattgcg
    58861 gttgtagtag cgtttttcca ttgaataggt gtttatttca ccgcttatca gcttttcaac
    58921 ctgttggaga tctttattga gatcctccgg ccaggtcagt tgctgaaagg tgagtccgcg
    58981 cagctcttcc tgactgtacc cgagaaactg gcagagcgct ttgttggttt gcagccattg
    59041 tccctcggtg cccactaacg ccataccgat agcggaatat tccatggcgt tccgaaaacg
    59101 ggtttcgctt tcggaaatgt gtttgcgttc cgcacgaaag gcatacatca ccatcgtcat
    59161 gatgttggcg ggcagcagga tcagcaaaaa cggtagccac ggcatatggc tcatcaggta
    59221 tgtacgcggc gtagcaagca gggagggatc cgcggccatc atcagcgaca ccatcatcac
    59281 cgtggtaagg aagatcaaaa aggcttccat tcgtggcagg cgcacggcgc tccacatcaa
    59341 cagcacaata atgaaagtaa aaggccacgg cagatacagc atcgaaagcc agcttaacgt
    59401 cagtgtgatg gctaacgtga gcagcgactc aaaaagcaac cgtgggttgc gatggcgcag
    59461 cagatagtgt ggtttaaata acaatcccag cggcaccagt gccagagcgc cgatggattc
    59521 tgacagtacc catatcaaaa atgccctgag aggatcgtct ccgggcgtca gcaggacaac
    59581 cagaacaccc cctagcagag gcggaacaat ggcgctgccg aatgccagac gcagccagtc
    59641 agccagattt tgcaaggggt tataccacgg cagcaattta cgtagcagca ctgccccgac
    59701 cacggcttca acaatattga tggtcgtcca ggtcatgttc agcgagctgg tggaaaaaag
    59761 caggatggat gcggcgatat ttcccagcga gcagctcagc gcaattcccg gccacatgcg
    59821 cccggcatgg cgataaaacg ccaccatcat gatggacgtc gggaaccaca gtggggcgag
    59881 ttgggtgcca aactgcgaaa gctcaagcga gaaaagggta aagataaacg agactaaacc
    59941 taaactgacc aggtgaagca gcgggtgggg cagggcaatt aatacatgct gtgattgttt
    60001 gctcattacc gctttatccg caagaccagg tcgccagtat tcgtttctgt gtttatgcta
    60061 gtacaaaagt tttacatttt atatgcgaat tgctcataaa gtgacgtaaa ggcggataat
    60121 ttgcgcaact gcgtataaca ttttttacct tgtataaaac tgatcaacgt aatttgccac
    60181 cgggaattct tcgtcaacaa ttaactctga gcgcgcggca gcgctattcg actggtatca
    60241 ggcggatgaa atccctataa ttgccgcgtt tggcgcttcg tcgccccctt cctaacatcc
    60301 aggttaatca ggtcgctaaa tatatgactg atcagtctca tcagtgcgtc attatcggta
    60361 tcgctggcgc atcggcttcc ggcaagagtc ttattgccag taccctttat cgtgaattac
    60421 gtgagcaagt cggtgatgaa cacatcggcg taattcccga agactgctat tacaaagatc
    60481 aaagccatct gtcgatggaa gaacgcgtta agaccaacta cgaccatccc agcgcgatgg
    60541 atcacagcct gttgcttgag catttacaag cgttgaaacg cggctcggca attgacctgc
    60601 cggtttacag ctatgttgaa catacgcgta tgaaagaaac ggtgacggtt gagccgaaga
    60661 aggtcatcat tctcgaaggc attttgttgc tgacggatgc gcgtttgcgt gacgaactta
    60721 acttctccat tttcgttgat accccgctgg atatctgcct gatgcgccgc atcaagcgtg
    60781 acgttaacga gcgtgggcgt tcaatggatt ccgtgatggc gcaatatcaa aaaaccgtgc
    60841 gcccgatgtt cctgcaattc attgagcctt ctaaacaata tgcggacatt atcgtgccgc
    60901 gcggcgggaa aaaccgcatc gcgatcgata tattgaaagc gaaaataagt cagttctttg
    60961 aataagcttg ataaattgtg taccgttcag tgataacctg gtatgccctt gacgtaattg
    61021 gcgttaaggg agtgatgcgc gaaaggagaa aatgccatgc gtctgtgtga ccgagatatt
    61081 gaagcctggc ttgatgaagg ccgtttgtcg atcaacccac gtccgccagt ggagcgtatt
    61141 aacggcgcga cggtggatgt acgcctgggc aataaatttc gtaccttccg tggtcacacg
    61201 gcagcgttta tcgatctgag cggtcccaaa gatgaagtga gcgccgcgct tgaccgcgtg
    61261 atgagcgatg agatcgttct cgacgagggc gaggcgttct atcttcaccc aggagagctg
    61321 gcgctggcgg tgacgctgga gtcggtgacg ctgccagccg atctggtggg ctggctggac
    61381 gggcgttcct ctttggcgcg tctggggctg atggtgcacg tcaccgcgca ccgtatcgat
    61441 ccgggctggt ctggttgcat tgtgctggag ttctacaact ccggtaagct gccgctggcg
    61501 ctgcgtccgg gcatgttaat tggtgcgctg agctttgagc cgctttccgg cccggcggcg
    61561 cgaccttaca accgccgtga agatgcgaaa tatcgcaacc agcagggcgc ggtagccagc
    61621 cgaatcgata aagactaatt caggcccatt gaggatacca tgagacgatt tctgacgacg
    61681 ctgatgatac tcctggtcgt gctggtggcc gggttatctg cgttagtgtt gctggtgaat
    61741 ccgaatgatt tccgcgacta tatggtcaag caagttgctg cacgtagcgg ttatcaattg
    61801 cagctcgacg ggccactgcg ttggcacgtc tggccgcagc ttagtatcct ctccgggcga
    61861 atgtctctca ccgcccaggg cgcaagccag ccactggttc gcgccgacaa catgcgtctg
    61921 gacgtggcgc ttttaccact actgagtcat caactgagcg ttaagcaggt gatgctaaaa
    61981 ggggcagtga tccaactgac gccgcagacg gaagcggtgc gcagtgaaga cgctccggtt
    62041 gcaccgcgcg acaatacctt gccggatctg tcagacgatc gcggatggtc gtttgatata
    62101 tccagtctta aggtggcgga cagcgtgctg gtgttccagc atgaagatga cgagcaggtg
    62161 acaatccgca atatccgcct gcaaatggaa caagatcccc aacatcgtgg ctcatttgag
    62221 ttctccgggc gggttaatcg cgatcagcgc gatctcacga tatcccttaa cggtacggta
    62281 gatgcttctg attatccgca tgatttaacg gcggctattg aacaaattaa ctggcagttg
    62341 cagggtgcag atttaccaaa acaaggtatt caggggcagg ggagtttcca ggcccagtgg
    62401 caggagtcac ataaacgcct ttcatttaac caaatcagct tgaccgccaa tgacagcacg
    62461 ctgagcgggc aagcacaggt cacgctgaca gagaaacctg aatggcagct gagactgcaa
    62521 ttcccgcaac tgaatcttga caacctcatc ccgcttaatg aaacagcgaa tggtgaaaac
    62581 ggtgccgcgc agcaggggca gagccaatca acattgccgc gcccggtcat ttcttcgcgt
    62641 attgatgaac cggcatatca gggactgcaa ggctttacgg ctgatatttt gttgcaggcc
    62701 agtaacgtgc gctggcgcgg aatgaatttt acagatgttg ccacgcaaat gaccaacaag
    62761 tcgggtttgc tggaaattac tcaactgcag ggcaagctta acggtggaca ggtttcactg
    62821 ccgggcacgc tggacgcgac atcaataaat ccgcggataa acttccagcc acggctggaa
    62881 aacgttgaga ttggtaccat tctgaaggcg tttaactatc cgatttcgtt gaccggaaaa
    62941 atgtcactgg ctggtgattt ctccggtgct gacatagatg ccgacgcatt ccgccacaac
    63001 tggcaaggac aggcacatgt cgaaatgacc gacacgcgca tggaagggat gaacttccag
    63061 cagatgattc agcaagcggt agaacgtaat ggtggtgatg tgaaggccgc tgaaaacttc
    63121 gataacgtaa cgcgtcttga ccgctttacc accgatttga cgttgaagga tggcgtcgtg
    63181 acgttaaacg acatgcaagg tcaatcgcct gtgctggcgc tgacagggga aggcatgttg
    63241 aatctggcag atcaaacctg cgacacccag tttgatattc gggtcgtggg tggctggaac
    63301 ggggaaagca aactgattga tttcctcaaa gaaacgccag taccgctgcg ggtttatggc
    63361 aactggcagc aactcaatta cagtctgcaa gtggatcagt tactgcgcaa acatctacag
    63421 gacgaagcga aacgtcgcct gaatgactgg gccgagcgga ataaagattc ccgcaatggc
    63481 aaagatgtga agaagttgct ggagaagatg taggttacca ggtgacctgt cctgtaggcc
    63541 tgataagacg cgtaaagcgt cgcatcaggc gttcgcggcg gcaccattat attctgaata
    63601 tgtaagatcg aaggctgttc cgttcacttg atgttcctca ctactatgga acgctcaagc
    63661 cggtgaacgt gccagggtgg ctcaatcgcc accaccctgg caacccgggc tcccggcggt
    63721 aaatcgccgc tggcgcggtt ccctcatctt attcctttcg gctatcgggt aaaatgaccg
    63781 ataccatcac acctcgtact ccaactcgcc atccttatgc agcggaataa tctgcacctt
    63841 ctgcacgcga tggctttcca cctgcaacgt tttaagcaaa taatccccca cctgaacttc
    63901 ttcgccaggt tttggaatac gctgtaaata ttccatcaac agcccggcaa tggtgtgata
    63961 ttcacgtttt tcatccagcg gcagcggcac atattgcacc agatcttcca gcggcatatg
    64021 accattcgcc gtccaggaac cgtccgcatt cttctgaata ccatggcggg cgtcgatctc
    64081 ttccacttcg ttcggtaagt taccggcaat ggtttcagtg acgtcactta atgtcacaat
    64141 cccttccacc gagccaaact catccactac aaaagcaaag tgcgtgcggg cattacggaa
    64201 ctgctccagg gcaggtaaca acggcaaggt ttccgggaac accagcggct ggcgaatcaa
    64261 cacccgcagg ttgagcggtt cgccgcgcag tgactgttgt agcaggtcga taacgtgaac
    64321 aacaccgagc aaatcttctg cgtcatcacc gtcggtaacc accagccgcg tatgctgatt
    64381 tcgctccagc aactggcgga tttcttcttc cggcgcgttg agatcgatat gctcaatatc
    64441 gtggcgcgac gtcataatgc tgctgacagt acgctggtta agattaagta cccgctcaat
    64501 catccgccgt tcctgcggat taaagatctg ctggttacca tgatccacca gcatggacgc
    64561 ggtttcggcg tccagttccg catcttcttt ttgcccgcta agcaggcgca ttaccgcttc
    64621 ggtagtccgc tggcgcagcg tctggttagc ggaaagaaaa cgtcgccggt taaagatagc
    64681 caactgattg agcgcctcga tcatcaccga gaaaccaatg gcagcgtaca ggtagccttt
    64741 cggaatgacg aagccgaaac cttctgccac caggctaaag ccaatcatca acaggaagct
    64801 taagcagaga ataacgatgg tcgggtgact gttaacaaat tgcgttaacg gcttgctggc
    64861 catcaacatc aggctgatag cgataaccac ggcggccatc atgaccagta aatggtcgac
    64921 catcccgacg gcggtaatca ctgagtcgag tgagaagatg gcgtccagta ccacaatttg
    64981 cgtcaccacg ccccagaact tcgcaccttt gcgttgtgtg ggattattgc tgtctttccc
    65041 ttccagccgt tcgttcagct ccatcgtggc tttgaacaac aggaagaaac caccaaacag
    65101 catgattaag tcgcgggcgc taaaggtaaa cgagcagaag ctgaacagcg gttgagtcag
    65161 ggtgaccagc caggagattg acgccagcag taacaggcgc attaacatcg ccagcagcag
    65221 cccggtaacc cgtgcgcggt cgcgctgctt cggcggtagt ttttcggcga ggatggcaat
    65281 aaagaccagg ttatcaatgc cgaggaccag ttcgatcaca atcagcgtga ttaacccggc
    65341 ccagatagac ggatcggcaa tccattccat agtaagtaca aaacctattc gttgtatgac
    65401 aaatgtcata attcgatcat ggataaatcg gggcaaaaat gcaatgttgc agcactattt
    65461 atctcatatt atttgagggt aataacctca cattatccct gaattaaaag tggtaataat
    65521 aaaacattat ttaagaaata tcgcatgtgg aggcgcaata ttaaatattg tcaacctaaa
    65581 gaaactccta aaaaccatat tgaatgacac ttaatataat tcttaaaaat agccaattac
    65641 cgaattgtta ccttgcctgc tattccgtta gctgtaacac ttcctcctgc attattggag
    65701 agccaatatt caattaccat aatgtcacct tatattcgga agttaatttc cgcaataacc
    65761 ctttctcatc aacgattgta tgcagagagg gtttgaaaaa ttaagctaaa tcagtgtatt
    65821 ggtagctaaa aagccagggg cggtagcgtg tctggatgcc tgaaagacca gtctgaatta
    65881 ttctgtcaat agcctgcgga tgaaatcaat ttttttagga ctgatgccag ttaaattttt
    65941 attcaattaa ctgcaaggta aatgtgcatg gcgacattgc tattaatagt gcacaggata
    66001 attactctgc caaagtgata aataaacaat gatgaaatcc aaaatgaaat tgatgccatt
    66061 attggtgtca gtaaccttga taagcggttg cacagtactt ccgggcagca atatgtcgac
    66121 gatgggcaaa gacgtcatca aacagcagga cgctgatttc gatctcgaca aaatggtgaa
    66181 tgtttatccg ctgaccccgc gcctgattga ccaattacgc ccacgcccga atgtagcgcg
    66241 ccccaatatg acgctggaaa gcgagatcgc gaattatcag tatcgcgtcg ggccggggga
    66301 cgttcttaat gtcaccgtct gggatcaccc ggaactcacc acgccagccg gtcagtaccg
    66361 cagctccagc gacaccggca actgggtaca gcctgacggc actatgtttt acccgtatat
    66421 cggcaaggtt cacgtagtcg ggaaaacgct cgctgaaatc cgcagtgata ttaccgggcg
    66481 cttagcgacg tacatcgctg acccgcaggt ggacgttaat atcgccgcct tccgctcgca
    66541 aaaggcctat atctcaggtc aggtgaataa atccggtcaa caggcgatca ccaacgtacc
    66601 actgaccatt ctcgacgcca tcaacgccgc aggtggcctg accgacaccg ctgactggcg
    66661 caacgtggtg ctaacacaca atggtcgtga agaacgcatt tctttgcagg cgctgatgca
    66721 aaacggcgac ctcaatcaga accgactgct ttatcccggc gatattctct acgtgccgcg
    66781 taatgatgat ctgaaagtat ttgtgatggg tgaagtgaag aaacagagca ccctgaaaat
    66841 ggactttagc ggcatgaccc tgactgaagc tctgggcaat gccgaaggca tcgacatgac
    66901 cacctccaac gccagcggca tctttgtcat tcgtccgttg aaaggcgagg gcgggcgtaa
    66961 cggcaagatt gccaatatct accagctgga tatgtccgat gcgacgtcgc tggtgatggc
    67021 gacagaattc cgcctgcaac cttatgacgt ggtgtatgtc accaccgccc cggtttcccg
    67081 ctggaaccgt ctgatcaatc agttgctgcc aactattagc ggtgtccgtt acatgacgga
    67141 tacagccagc gacattcata actggtaatc gtcatgttta acaacatctt agttgtctgt
    67201 gtcggcaata tttgccgttc cccgacggcg gaacgcttac tgcaacgtta tcacccggag
    67261 ctgaaagtgg agtccgctgg actcggcgcg ctggtcggta agggcgctga tcctaccgct
    67321 atcagcgtcg ccgcagaaca tcaactgtct ctggaaggtc actgtgcacg gcaaatcagc
    67381 cgccgcctgt gtcgcaacta tgacctgatt ttgaccatgg aaaagcgcca tatcgaacgc
    67441 ttatgcgaga tggcacctga gatgcgcggc aaagtgatgc tgtttggtca ctgggataac
    67501 gaatgtgaaa tccccgatcc gtatcgcaaa agccgggaaa cgtttgcagc ggtgtacaca
    67561 ttacttgaac ggtctgcccg ccagtgggcg caggcattga acgcagagca ggtataagaa
    67621 tgacagaaaa agtaaaacaa catgccgctc cggtaacggg cagtgatgaa atcgatattg
    67681 gtcgcctggt cggcaccgtc attgaagcgc gctggtgggt gattggcatc accgctgtat
    67741 ttgccctctg tgctgtggtt tacactttct tcgccacgcc gatttatagt gccgacgcac
    67801 tggtacaaat cgaacaaaac agcggcaatt cgttagtgca ggacattggc tcggcattag
    67861 ccaacaaacc gcctgcatcg gacgccgaga tccagttgat tcgttcgcgt ctggtgcttg
    67921 gtaaaacggt ggacgatcta gacctcgata ttgcggtgag caaaaacacg ttcccgattt
    67981 tcggtgcggg ctgggatcgt ttgatgggac gtcagaacga gacggtgaaa gtgaccacct
    68041 ttaaccgccc gaaagagatg gcggaccagg tgtttacgct taatgtgctg gacaataaaa
    68101 actacaccct gagcagtgat ggcggcttta gcgcccgtgg gcaagcgggc cagatgctga
    68161 aaaaagaagg cgtcacgctg atggttgaag ccattcacgc ccgcccgggc agtgagttta
    68221 ccgtcaccaa atactccacg ctggggatga tcaatcaact gcaaaacagc ctgacggtaa
    68281 cggagaacgg caaagacgca ggcgtactga gcctgactta taccggtgaa gatcgtgaac
    68341 agatccgcga cattcttaac agcatcgccc gtaactatca ggaacaaaat attgagcgca
    68401 aatcggcgga agcgtcgaaa agcctcgctt tcctcgcgca acagttaccg gaagtacgta
    68461 gccgcctcga tgttgccgaa aacaaactga atgccttccg tcaggataaa gattctgttg
    68521 atctgccgct ggaagcgaaa gcggtgctcg attcgatggt gaacatcgac gcgcagttga
    68581 acgaactgac ctttaaagag gcggaaatct ccaagctgta caccaaagtt caccccgcgt
    68641 accgcacgct gctggagaaa cgtcaggcgc tggaagacga aaaagccaaa cttaatggtc
    68701 gcgtaacggc gatgccgaaa acccagcagg aaattgtccg tctgacccgc gatgtcgaat
    68761 ctggtcagca ggtctatatg caactgctga ataaagagca ggagctgaaa atcaccgaag
    68821 ccagcaccgt cggcgatgtg cgcattgttg acccggcaat cactcagcct ggtgtgctaa
    68881 aaccgaagaa agggctgatt atcctcggcg cgattatcct tggcctgatg ctctctatcg
    68941 tgggtgtgct gctgcgctcg ttgtttaatc gcggcatcga aagcccgcag gtgctggaag
    69001 aacacggtat cagcgtctat gccagcatcc cgttgtcgga atggcagaaa gcgcgcgata
    69061 gcgtcaaaac catcaaaggg attaaacgct ataaacagag ccagctactg gcggtgggga
    69121 atccaaccga tctggcgatt gaagccatcc gcagtcttcg taccagtttg cacttcgcga
    69181 tgatgcaggc gcagaacaat gtgttgatga tgaccggggt tagcccgtca atcggtaaaa
    69241 cctttgtctg cgccaacctg gcggcggtga tcagccagac caataaacgc gtgttgttga
    69301 tcgactgcga tatgcgcaaa ggctacaccc acgagctgtt gggcactaat aacgttaatg
    69361 gcctgtcgga aattctgatt ggtcagggcg atattactac cgctgctaaa ccgacctcta
    69421 ttgccaaatt tgacctgatc ccgcgcggtc aggtgccgcc aaatccttct gaactgttga
    69481 tgagcgaacg ctttgccgaa ctggtgaact gggcgagtaa aaactatgac ctggtgttga
    69541 ttgatacgcc gccgattctg gcagtgaccg atgcggcaat tgttggtcgt catgttggaa
    69601 ccacgttaat ggtggcgcgt tatgcggtca acacattgaa agaagtggaa accagtctga
    69661 gccgctttga gcaaaatggt attccggtga aaggggtgat tctgaactcc atcttccgcc
    69721 gcgccagcgc gtatcaggat tatggctatt acgaatacga atataagtcg gatgcgaaat
    69781 aacgacgcct gcattcgcac cgccccgtag gccggataag gcgctcacgc cgcatccggc
    69841 aagcaaacca gctcataagc cgggagaaca acctatgaaa gacaatccgc tgatctcaat
    69901 ctatatgcca acctggaacc gccaacaact ggcgattcgg gcaataaaat cggttttgcg
    69961 ccaggactac agcaactggg agatgatcat cgtggatgat tgctccacct cctgggaaca
    70021 actgcaacag tacgtcaccg ccctcaacga tccgcgtatc acttacattc acaacgatat
    70081 taacagcggg gcgtgcgcgg tacgtaacca ggcgattatg ctggcgcaag gggaatatat
    70141 caccgggatt gatgacgatg acgaatggac acccaaccgt ctgagcgtct tcctcgccca
    70201 taaacagcaa ctggtcacac acgccttttt gtacgctaac gactacgtat gccagggaga
    70261 agtttattcc caaccggcaa gcttaccact gtatcccaaa tcgccgtact cacgccgcct
    70321 gttctataaa cgcaatatca ttggcaatca ggtctttacc tgggcatggc gtttcaaaga
    70381 gtgtttgttc gataccgaac tgaaagcagc acaggattac gacatcttcc tgcggatggt
    70441 ggtggagtac ggcgaaccgt ggaaagtgga agaggcgacg cagatcctgc atatcaatca
    70501 cggtgaaatg cagatcacct cgtcaccgaa aaaattctcc gggtacttcc acttttaccg
    70561 caagcacaaa gataaatttg accgtgcaag caaaaaatat cagctgttta ccctctacca
    70621 gatccgcaat aaacgtatga cctggcgcac tttgctgacg ctgctgtcgg tgcgtaacgg
    70681 caaacgtctg gctgatggta ttcgggggcg ctaaagatgc tggaagatct gcgtaccaac
    70741 agctggagtt tacgcccgtg ctgcatggtt cttgcctatc gtgttgctca tttttgttcg
    70801 gtgtggcgca aaaagaacgt cctcaacaat ctgtgggcgg ccccgctgct ggtgctgtat
    70861 cgcattatca ccgaatgctt tttcggttat gaaatccagg ccgccgcgac cattggccgc
    70921 cgctttacta tccatcacgg ttacgccgta gtgatcaata aaaacgtggt agcgggggat
    70981 gatttcacca ttcgccacgg cgtcactatc ggcaatcgtg gtgccgataa catggcatgt
    71041 ccacacattg gcaacggcgt cgaactcggt gccaacgtca ttattcttgg tgatatcacg
    71101 cttggtaaca acgtcaccgt gggcgcgggc agcgtggtgc tcgactctgt cccggacaac
    71161 gcgctggtgg tgggagaaaa agcgcgagtg aaggtaatta aatgaatatt ctgcaattta
    71221 atgtgcgact ggcggaaggc ggggcggcag gtgtggcgtt agatctccat cagcgtgcgc
    71281 tgcaacaggg gctggcgtca cattttgtct acggctacgg caaaggcggc aaagagagcg
    71341 tcagccatca aaactatccg caggtcgtca aacatacgcc gcggatgacc gcgatggcga
    71401 acattgcgtt gtttcgtctg tttaatcgcg atctgtttgg caatttcaat gagttatatc
    71461 gcaccattac ttgcacaccg ggtccggtgg tcctgcattt tcatgtgctg cacagctact
    71521 ggctaaatct taagagcgtg gtgcgctttt gcgaaaaggt gaaaaaccac aaaccggatg
    71581 tcactctggt ctggacgctg cacgaccact ggagcgttac cgggcgctgc gcctttactg
    71641 acggttgtga aggctggaaa acgggctgcc agaaatgccc gaccttaaat aattatccgc
    71701 cggtgaagat tgatcgggcg caccagctgg tggcgggcaa acgccagtta ttccgtgaga
    71761 tgctggcgct gggctgtcag tttatttccc ccagccagca tgtggctgac gctttcaata
    71821 gcctgtacgg tccagggcgt tgccggatta tcaataacgg tattgatatg gcaaccgaag
    71881 cgattctggc ggatctgcct ccggtgcgcg aaacccaggg taagccgaaa atcgcggtgg
    71941 tggcgcatga cctgcgttac gacggcaaaa ctaaccagca actggtgcgt gagatgatgg
    72001 cgctgggcga caaaatcgaa ctgcatacct ttggtaagtt ctcgccgttc accgctggca
    72061 acgtggttaa tcacggcttt gaaactgaca agcgcaagtt gatgagcgcg ctcaatcaga
    72121 tggatgcgct ggtgttcagt tctcgcgtcg ataactaccc gctgattttg tgtgaggcgc
    72181 tatcgattgg cgtgccggtg attgccaccc atagcgacgc ggcgcgggaa gtgctgcaaa
    72241 aatccggcgg taaaaccgtc agcgaagaag acgtgctgca actggtgcag ttaagcaaac
    72301 cggaaatcgc gcaggcgata tttggtacca cgctggctga gttcagccaa cgcagccgcg
    72361 cggcctacag tggacaacag atgctggagg agtatgtcaa cttctatcag aatctgtagc
    72421 tacctgttgt tgccgctgat ttatctgctg gttaacgtca aaatcgccca gcttggcgaa
    72481 agtttcccca tcaccatcgt cactttttta cctgtcttgt tactgctgtt tttagagcgc
    72541 atcagcgtta aaaaattgat gattgcctta gggattggtg cgggactcac ggcgtttaac
    72601 tatctgtttg gtcagtcgct ggatgccagt aaatacgtca cttcaactat gctgtttgtc
    72661 tatattgtga tcattattgg catggtgtgg agtattcgtt ttaaaacaat ttcgccacac
    72721 aaccatcgca agatattacg tttcttttat ctggtggtcg ggttggtggt ggcgctggcg
    72781 gcggtggaga tggcgcaaat tatcctcacc ggcggcagca gtattatgga gtcgatttcg
    72841 aaatatctga tttacagcaa cagctatgta ctgaatttca ttaaattcgg cggcaagcgc
    72901 acgacagcac tttatttcga accggcattt ttcgctctgg cattaatctc aatttggctc
    72961 agcatcaaac agtttggtat caaaacgcct aaaaccgatg ctatgattct cgcagggata
    73021 atattatccg gatcgttttc aggggttatg acctttatcc tgttttattt gctggagtgg
    73081 gcatttcaat atctgaataa agaggcgatt aagaaaaagt taccgttagc attgatttct
    73141 ctggctgtat tcctggttgg tgtggtaatc gcgtttcctt atatttccac ccgtctgggt
    73201 gatttaggta cggaaggatc gtcatcgtat tatcgtattg tcggtccgct ggtgatggtc
    73261 ggttattctt tgacccatat tgacggtgta gtcagatttg gctcacttta tgaatatgtc
    73321 gcatcattcg gaatatttaa cggtgcggat gtcggaaaaa ccatagacaa tggtttgtat
    73381 ctgctgatta tttatttttc ctggttcgca gtgtttttat cgctgtggta catggggaaa
    73441 gtgataaaaa tgatgatcaa cgcttttggt gataaccgca attttcgcgt gcaattatat
    73501 ctttttactc cggtgtcgct gttttttacc ggttcgatat ttagcccgga atatgcattt
    73561 ttaatcgtct gtccgtttat tttgcgaaaa gcgttaaata ttacgaggta agaataagaa
    73621 catgttgctt agcataatca ctgtcgcgtt tcgtaacctc gaagggatag tcaaaacaca
    73681 tgcctcgctg gcgcatctgg cgcaggcgga agatatcagc ttcgaatgga ttgttgtcga
    73741 tggcggttcc aatgacggca cccgtgagta tctggaaaat ctcaatggta tctataacct
    73801 acgctttgtc agcgagccag ataacggtat ctacgacgcc atgaacaaag gcattacgat
    73861 ggcacaaggc aagttcgcgt tgtttctcaa ttcgggcgat atttttcatc aggatgccgc
    73921 aaattttgtc cgtaagttaa aaatgcaaaa agataacatg atgatcaccg gcgatgcgct
    73981 gctggatttt ggcgacgggc ataaaattaa acgtagcgcc aaaccgggct ggtatattta
    74041 tcacagcctg cccgccagtc atcaggcgat atttttccct gtatccggct tgaaaaaatg
    74101 gcgttatgac ctggaatata aagtttcttc cgactacgcg ctggcagcca aaatgtataa
    74161 agccggttat gcatttaaaa aactcaatgg cctggtgtct gaattttcca tgggtggggt
    74221 atctaccacc aataatatgg aattgtgtgc tgacgcgaaa aaagtccaac gacaaatatt
    74281 acatgtacct ggcttttggg ctgaattatc ctggcattta cgccaacgta ctacctcaaa
    74341 gacgaaagcc ttatataaca aagtctgaat ataaggagaa ccaatgcaag atttaagcgg
    74401 tttctcggtg ccgaaagggt tccggggcgg caacgctatt aaagtgcaat tatggtgggc
    74461 agtacaggca acaatatttg cctggtcgcc acaagtattg tatcgctggc gggctttttt
    74521 attacgttta ttcggagcaa aaataggaaa aaacgtagtt attcgtccgt cagtaaaaat
    74581 tacctatccg tggaaattaa ccttaggtga ttacgcgtgg gtcggcgatg acgtcaattt
    74641 atataccctc ggtgaaataa ccattggcgc acattcggtg atatcgcaaa aaagttattt
    74701 atgcaccggt agccacgacc atgcaagtca acatttcacc attaacgcca cgcctattgt
    74761 gattggcgag aaatgctggc tggcaaccga tgtctttgtt gccccaggcg tcacaatcgg
    74821 caacggcacc gtcgtgggtg cacgaagcag tgtttttaaa tcgcttccgg caaatgtggt
    74881 ttgccggggg aatcccgcag tggtgatacg cgaacgcgtt gaaactgaat aaattcaaaa
    74941 atacagagga ataagacatg tcaaaagtcg ctctcatcac cggtgtaacc ggacaagacg
    75001 gttcttacct ggcagagttt ctgctggaaa aaggttacga ggtgcatggt attaagcgtc
    75061 gcgcatcgtc attcaacacc gagcgcgtgg atcacattta tcaggatccg cacacctgca
    75121 acccgaaatt ccatctgcat tatggcgacc tgagtgatac ctctaacctg acgcgcattt
    75181 tgcgtgaagt acagccggat gaagtgtaca acctgggcgc aatgagccac gttgcggtct
    75241 cttttgagtc accagaatat accgctgacg tcgacgcgat gggtacgctg cgcctgctgg
    75301 aggcgatccg cttcctcggt ctggaaaaga aaactcgttt ctatcaggct tccacctctg
    75361 aactgtacgg tctggtgcag gaaattccgc agaaagaaac cacgccgttc tacccgcgat
    75421 ctccgtatgc ggtcgccaaa ctgtacgcct actggatcac cgttaactac cgcgaatcct
    75481 acggcatgta cgcctgtaac ggtattctct tcaaccatga atccccgcgc cgcggcgaaa
    75541 ccttcgttac ccgcaaaatc acccgcgcaa tcgccaacat cgctcagggg ctggagtcgt
    75601 gcctgtacct cggcaatatg gattccctgc gtgactgggg ccacgccaaa gactacgtaa
    75661 aaatgcagtg gatgatgctg caacaggaac agccagaaga tttcgttatc gcgaccggcg
    75721 ttcagtactc cgtacgtcag ttcgtggaaa tggcggcagc acagctgggc atcaaactgc
    75781 gctttgaagg cacgggcgtt gaagagaagg gcattgtggt ttccgtcacc gggcatgacg
    75841 cgccgggcgt taaaccgggc gatgtgatta tcgccgttga cccgcgttac ttccgtccgg
    75901 cggaagttga aacgctgctc ggcgacccga ccaaagcgca cgaaaaactg ggctggaaac
    75961 cggaaatcac cctcagagag atggtgtctg aaatggtggc taatgacctc gaagcggcga
    76021 aaaaacactc tctgctgaaa tctcacggct acgacgtggc gatcgcgctg gagtcataag
    76081 catgagtaaa caacgaattt ttatcgctgg ccatcgcggg atggtcggtt ccgccatcaa
    76141 gcgacagctc gaacagcgcg gtgatgtgga actggtatta cgcacccgcg acgagctgaa
    76201 cctgctggac agccgcgccg tgcatgattt ctttgccagc gaacgtattg accaggtcta
    76261 tctggcggcg gcgaaagtgg gcggcattgt tgccaacaac acctatccgg cggatttcat
    76321 ctaccagaac atgatgattg agagcaacat cattcacgcc gcgcatcaga acgacgtgaa
    76381 caaactgctg tttctcggat cgtcctgcat ctacccgaaa ctggcaaaac agccgatggc
    76441 agaaagcgag ttgttgcagg gcacgctgga gccgactaac gagccttatg ctattgccaa
    76501 aatcgccggg atcaaattgt gcgaatctta caatcgccag tacggacgag attaccgttc
    76561 agtcatgccg accaacctgt acgggccaca cgacaacttc cacccgagta attcgcatgt
    76621 gatccctgca ttgctgcgtc gcttccacga ggcgacggca cagaatgcgc cggacgtggt
    76681 ggtgtggggc agcggtacac cgatgcgtga attcctgcac gtcgatgata tggcggcggc
    76741 aagcattcat gtgatggagc tggcgcacga agtctggctg gagaacaccc agccgatgct
    76801 gtcgcacatt aacgtcggca cgggcgttga ctgcactatc cgcgagctgg cgcaaaccat
    76861 cgccaaagtg gtgggttaca aaggtcgggt ggtttttgat gccagcaaac cggatggcac
    76921 accgcgcaaa ctgctggatg tgatgcgcct gcatcagctt ggctggtatc acgaaatatc
    76981 actggaagcg gggcttgcca gcacttacca gtggttcctt gagaatcaag accgctttcg
    77041 ggggtaatga tgtttttacg tcaggaagac tttgccacgg tagtgcgctc cacaccgctt
    77101 gtctctctcg actttattgt cgagaacagt cgcggcgagt ttctgcttgg caaaagaacc
    77161 aaccgcccgg cgcagggtta ctggtttgtg ccgggagggc gcgtgcagaa agacgaaacg
    77221 ctggaagccg catttgagcg gctgacgatg gcggaactgg ggctgcgtct gccgataaca
    77281 gcaggccagt tttacggtgt ctggcagcac ttttatgacg ataacttctc cggcacggat
    77341 ttcaccactc actatgtggt gctcggtttt cgcttcagag tcgctgaaga agatctgtta
    77401 ctgccggatg agcagcatga cgattaccgc tggctgacgc cggacgcgct gctcgccagt
    77461 gacaatgttc acgctaacag ccgcgcctat tttctcgctg agaagcgtgc cggagtaccc
    77521 ggattatgaa aatactggtc tacggcatta actactcgcc ggagttaacc ggcatcggca
    77581 aatacaccgg cgagatggtg gaatggctgg cggcacaagg tcatgaggtg cgggtcatta
    77641 ccgcaccgcc ttactacccg cagtggcagg tgggcgagaa ctattccgcc tggcgctaca
    77701 aacgagaaga gggggccgcc acggtgtggc gctgcccgct gtatgtgcca aaacagccga
    77761 gcaccctgaa acgcctgttg catctcggca gttttgccgt cagcagtttt ttcccgctga
    77821 tggcgcaacg tcgctggaag ccggagcgca ttatcggcgt agtgccaacg ctgttttgca
    77881 cgccgggaat gcgtctgctg gcgaaactct ctggcgcgcg taccgtgctg catattcagg
    77941 attacgaagt agatgccatg ctggggctgg gccttgccgg aaaaggcaaa ggcggcaaag
    78001 tggcacagct ggcaacggcg ttcgaacgta gcggactaca taacgtcgat aacgtctcca
    78061 cgatttcgcg ttcgatgatg aataaagcca tcgaaaaagg cgtggcggcg gaaaacgtca
    78121 tcttcttccc caactggtcg gaaatcgccc gttttcagca tgttgcagat gccgatgttg
    78181 atgcccttcg taaccagctt ggcctgccgg ataacaaaaa aatcattctt tactccggca
    78241 atattggtga aaagcagggg ctggaaaacg ttattgaagc tgccgatcgc ctgcgcgatg
    78301 aaccactgat ttttgccatt gtcgggcagg gtggcggcaa agcgcgactg gaaaaaatgg
    78361 cgcagcagcg tggactgcac aacatgcaat ttttcccgct gcaatcgtat gacgctttac
    78421 ccgcactgct gaagatgggc gattgccatc tggtggtgca aaaacgcggc gcggcagatg
    78481 ccgtattgcc gtcgaaactg accaatattc tggcggtagg cggtaacgcg gtgattaccg
    78541 cagaggccca cacagaactg ggggagcttt gcgaaacctt ttcgggcatt gcggtttgcg
    78601 tagaaccgga atcggtcgag gcgctggtgg cggggatccg tcaggcgctc ctgctgccca
    78661 aacacaacac ggtggcacgt gaatatgccg aacgcacgct cgataaagag aacgtgttac
    78721 gtcaatttat aaatgatatt cggggataat tatggcgcag tcgaaactct atccagttgt
    78781 gatggcaggt ggctccggta gccgcttatg gccgctttcc cgcgtacttt accccaagca
    78841 gtttttatgc ctgaaaggcg atctcaccat gctgcaaacc actatctgcc gcctgaacgg
    78901 cgtggagtgc gaaagcccgg tggtgatttg caatgagcag caccgcttta ttgtcgcgga
    78961 acagctgcgt caactgaaca aactcaccga gaacattatt ctcgaaccgg cagggcgtaa
    79021 cactgcacct gccattgcac tggcggcgct ggcggcaaaa cgtcatagcc cggagagcga
    79081 cccgttaatg ctggtattgg cggcggatca tgtgattgcc gatgaagacg cgttccgtgc
    79141 cgccgtgcgt aatgccatgc catatgccga agcgggcaag ctggtgacct tcggcattgt
    79201 gccggatcta cctgaaaccg gttatggcta tattcgtcgc ggtgaagtgt ctgcgggtga
    79261 gcaggatacg gtggcctttg aagtggcgca gtttgtcgaa aaaccgaatc tggaaaccgc
    79321 ccaggcctat gtggcaagcg gcgaatatta ctggaacagc ggcatgttcc tgttccgcgc
    79381 cggacgctat ctcgaagaac tgaaaaaata tcgcccggat atcctcgatg cctgcgaaaa
    79441 agcgatgagc gccgtcgatc cggatctcga ttttattcgc gtggatgaag aagcgtttct
    79501 cgcctgcccg gaagagtcgg tggattacgc ggtcatggaa cgtacggcag atgccgttgt
    79561 ggtgccgatg gatgcgggct ggagcgatgt cggttcctgg tcttcattat gggagatcag
    79621 cgcccacacc gccgagggca acgtttgcca cggcgatgtg attaatcaca aaacagaaaa
    79681 cagctatgtg tatgccgaat ctggcctcgt aaccaccgtc ggggtgaaag atctggtggt
    79741 agtgcagacc aaagatgcgg tgctgattgc cgaccgtaac gcggtgcagg atgtgaaaaa
    79801 agtggtcgag cagatcaaag ccgatggtcg ccatgagcat cgggtacatc gcgaagtgta
    79861 tcgtccgtgg ggcaaatatg actctatcga cgcgggcgac cgctaccagg tgaaacgcat
    79921 caccgtgaaa ccgggcgaag gcttgtcggt acagatgcac catcaccgcg cggaacactg
    79981 ggtggtggtc gcgggaacgg caaaagtcac tattgacggt gatatcaaac tgcttggtga
    80041 aaacgagtcc atttatattc cgctgggggc gacgcactgc ctggaaaacc cggggaaaat
    80101 tccgctcgat ttaattgaag tgcgctccgg ctcttatctc gaagaggatg atgtggtgcg
    80161 cttcgcggat cgctacggac gggtgtaaac gtcgcatcag gcgctgtttg tcggatgcgg
    80221 cgtgaacgcc ttatccgacc tacggttcgg atttgtaggc ctgataagac gcggcagcgt
    80281 cgcatcaggc aatgaatgcg aaaccgcgat gtaaataacg acaaaaacaa aattggccgt
    80341 ttcggtcagg gccaactatt gcctgaaaaa gggtaacgat atgaaaaaat taacctgctt
    80401 taaagcctat gatattcgcg ggaaattagg cgaagaactg aatgaagata ttgcctggcg
    80461 cattggtcgc gcctatggcg aatttctcaa accgaaaacc attgtgttag gcggtgatgt
    80521 ccgcctcacc agcgaaacct taaaactggc gctggcgaaa ggtttacagg atgcgggcgt
    80581 cgatgtgctg gatattggca tgtccggcac cgaagagatc tatttcgcca cgttccatct
    80641 cggcgtggat ggcggcattg aagttaccgc cagccataat ccgatggatt ataacggcat
    80701 gaagctggtt cgcgaggggg ctcgcccgat cagcggagat accggactgc gcgacgtcca
    80761 gcgtctggct gaagccaacg acttccctcc cgtcgatgaa accaaacgcg gtcgctatca
    80821 gcaaatcaac ctgcgtgacg cttacgttga tcacctgttc ggttatatca acgtcaaaaa
    80881 cctcacgccg ctcaagctgg tgatcaactc cgggaacggc gcagcgggtc cggtggtgga
    80941 cgccattgaa gcccgcttta aagccctcgg cgcacccgtg gaattaatca aagtgcacaa
    81001 cacgccggac ggcaatttcc ccaacggtat tcctaaccca ctactgccgg aatgccgcga
    81061 cgacacccgc aatgcggtca tcaaacacgg cgcggatatg ggcattgcct ttgatggcga
    81121 ttttgaccgc tgcttcctgt ttgacgaaaa agggcagttt attgagggtt actacattgt
    81181 cggcctgctg gcagaagcgt tcctcgaaaa aaatcccggc gcgaagatca tccacgatcc
    81241 tcgtctctcc tggaataccg ttgatgtggt gactgccgca ggcggcaccc cggtaatgtc
    81301 gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag gaagacgcca tctacggtgg
    81361 cgaaatgagc gcccaccatt acttccgtga tttcgcttac tgcgacagcg gcatgatccc
    81421 atggctgctg gtcgccgaac tggtgtgcct gaaaggaaaa acgctgggcg aactggtgcg
    81481 cgaccggatg gcggcgtttc cggcaagcgg tgagatcaac agcaaactgg cgcaacccgt
    81541 tgaggcgatt aaccgcgtgg aacagcactt tagccgtgag gcgctggcgg tggatcgcac
    81601 cgatggcatc agcatgacct ttgccgactg gcgctttaat ctgcgctcct ccaacaccga
    81661 accggtggtg cggttgaatg tggaatcgcg cggtgatgta ccgctgatgg aagcgcgaac
    81721 gcgaactctg ctgacgttgc tgaacgagta atgtcattac tttccccctc accctgacgc
    81781 tctactgcct gcggagagaa gtgagcacgg aactttcttc tccccctcgc ccctttgggg
    81841 agagggccgg ggtgaggggc gaggcgacaa tagaacagga acaacgatga caaatctaaa
    81901 aaagcgcgag cgagcgaaaa ccaatgcatc gttaatctct atggtgcaac gcttttcaga
    81961 tatcaccatc atgtttgccg gactatggct ggtttgcgaa gtgagcggac tgtcattcct
    82021 ctacatgcac ctgttggtgg cgttgattac gctggtggtg ttccagatgc tgggcggcat
    82081 caccgatttt tatcgctcat ggcgcggtgt tcgggcagcg acagaatttg ccctgttgct
    82141 acaaaactgg accttaagcg tgattttcag cgccggactg gtggcgttca ataatgattt
    82201 cgacacgcaa ctgaaaatct ggctggcgtg gtatgggctg accagcatcg gactggtggt
    82261 ttgccgttca tgtattcgca ttggcgcggg ctggctgcgt aatcatggct ataacaagcg
    82321 tatggtcgcc gtagcggggg atttagccgc cggacaaatg ctgatggaga gcttccgtaa
    82381 tcagccgtgg ttagggtttg aagtggtggg cgtttaccac gacccgaaac cgggtggcgt
    82441 ttctaacgac tgggcgggca atctgcaaca gctggtcgag gatgcgaaag cgggcaagat
    82501 tcataacgtc tatatcgcga tgcaaatgtg cgacggcgcg cgagtgaaaa aactggtcca
    82561 tcaactggcg gacactacct gttcggtgct gctgatcccc gatgtcttta ccttcaacat
    82621 tctccattcc cgtattgaag agatgaacgg cgtaccagtg gtgccgctgt atgacacgcc
    82681 gctttccggg gttaaccgcc tgctcaaacg tgcagaagac attgtgctgg caacgctgat
    82741 tctgctgctg atctccccgg tgctgtgctg cattgcgttg gcggtgaaac tgagctcacc
    82801 ggggccggtt attttccgcc agactcgcta cggcatggac ggcaagccga tcaaagtgtg
    82861 gaagttccgt tcgatgaaag tgatggagaa cgacaaagtg gtgactcagg cgacgcagaa
    82921 cgatccgcgc gtcaccaaag tggggaactt tctgcgccgc acctcgctgg acgaattgcc
    82981 gcagtttatc aatgtgctga ccggggggat gtcgattgtc ggtccacgtc cgcacgcagt
    83041 agcgcataac gaacagtatc gccagctcat tgaaggctac atgctgcgcc acaaagtgaa
    83101 accgggcatt accggctggg cgcagattaa tggctggcgc ggcgaaaccg acacgctgga
    83161 gaaaatggaa aaacgcgtcg agttcgacct tgagtacatc cgcgaatgga gcgtctggtt
    83221 cgatatcaaa atcgttttcc tgacggtatt caaaggcttc gttaacaaag cggcatattg
    83281 atatgagctt acgtgaaaaa accatcagcg gcgcgaagtg gtcggcgatt gccacggtga
    83341 tcatcatcgg cctcgggctg gtgcaaatga ctgtgctggc gcggattatc gacaaccacc
    83401 agttcggttt gctgactgtg tcgctggtga ttatcgcgct ggcagatacg ctttctgact
    83461 tcggtatcgc taactccatt attcagcgaa aagaaatcag ccaccttgaa ctcaccacgt
    83521 tgtactggct gaacgtcggg ctggggatcg tggcgtgtgt ggcggtattt ttgttgagtg
    83581 atgccattgg cgacatgctg aataacccgg acctggcacc gttgattaaa acattgtcac
    83641 tagcgtttgt ggtgatcccc cacgggcaac agttccgcgc gttgatgcaa aaagagctgg
    83701 agttcaacaa aatcggcatg atcgaaacca gcgcggtgct ggcgggcttt acttttacgg
    83761 tggttagcgc ccatttctgg ccgctggcga tgaccgcgat cctcggttat ctggtcaata
    83821 gtgcggtgag aacgctgctg tttggctact ttggccgcaa aatctatcgc cccgctctgc
    83881 atttctcgct ggcgtcggtg gcaccgaact tacgctttgg tgcctggctg acggcggaca
    83941 gcatcatcaa ctatctcaac accaaccttt caacactcgt gctggcgcgt attctcggcg
    84001 cgggcgtggc tgggggatac aacctggcgt acaacgtggc cgttgtgccg ccgatgaaac
    84061 tgaacccgat catcacccgc gtgttgtttc cggcgtttgc caaaattcag gacgataccg
    84121 aaaagctgcg cgtcaacttc tacaagctgc tgtcggtagt ggggattatc aactttccgg
    84181 cgctgctggg gctgatggtg gtgtcgaata actttgtacc gctggtattt ggcgaaaagt
    84241 ggagcagcat tattccggtg ctacagctgc tgtgtgtggt gggtctgctg cgctccgtgg
    84301 gtaacccgat tggttcgctg ctgatggcga aagcgcgggt cgatatcagc tttaaattca
    84361 acgtatttaa aacatttctg tttattccgg cgattgttat tggtgggcag atggcgggcg
    84421 caatcggcgt cacgcttggc ttcctgctgg tgcagattat caacactatt ctgagctatt
    84481 tcgtgatgat taaaccggtt cttggttcca gttatcgcca gtacatcctg agtttgtggc
    84541 tgccgtttta tctctcgctg ccaacgctgg tggtcagtta tgcgctgggc attgtgctga
    84601 aagggcaact ggcgctgggg atgctgctgg cggtgcaaat agccgcgggt gtgctggcgt
    84661 ttgtggtgat gattgtgctg tcgcgccatc cgctggtggt ggaagtgaag cgtcagtttt
    84721 gtcgcagcga aaaaatgaaa atgcttttac gggcggggtg agttgttgtt ttcggttccg
    84781 acaacggttg ccgggtgcag cgtaaaacgc cttatccgtc ctacggttct gtgctcgcat
    84841 caggcaaaaa acggcaacga acacaacaaa cacagcctca cctggcatca aatttatgag
    84901 gaaaaaatga aattacttat tctgggcaac cacacttgcg gcaatcgtgg cgacagcgcc
    84961 atcttgcgcg gcttacttga tgccatcaac atcctcaatc cacatgccga agtggacgtg
    85021 atgagccgct atcctgtcag ttcttcctgg ctactcaacc gcccggtaat gggcgatccg
    85081 ctgttcctgc aaatgaaaca acacaacagc gcggcgggcg ttgtcgggcg cgttaaaaaa
    85141 gtcctccgtc gccgctacca gcatcaggta ttgctctcac gcgtcaccga cagtggcaag
    85201 ctgcgtaata tcgccatcgc ccagggattc accgacttcg tgcgcctgct gtcaggttac
    85261 gacgccatta ttcaggtcgg cggatcgttt tttgtcgatc tctacggcgt gccacagttt
    85321 gaacatgcac tttgcacgtt tatggcgaaa aagtcgctgt ttatgattgg tcacagtgtt
    85381 ggcccgttcc aggatgagca atttaaccaa ctggcgaact acgtttttgg tcactgcgac
    85441 gcgctgatcc tgcgcgaatc ggtcagcctt gaactgatga aacgcagcaa tatcaccact
    85501 tcaaaagttg aacatggcgt cgataccgcg tggctggtcg atcaccacac agaagacttc
    85561 accgccagct atgctgttca acactggctg gacgttgccg cacaacagaa aacggtggca
    85621 attaccctgc gcgaactggc accgttcgac aaacgtctcg gcaccactca acaagcatat
    85681 gaaaaagcct ttgccggggt ggtcaatcgc attctcgacg aaggctatca ggtgattgcg
    85741 ctctccacct gtacgggtat cgacagctat aacaaagacg accgcatggt ggcgctcaac
    85801 ctgcgccagc acatcagcga tcctgcccgt taccacgtag tgatggatga actcaacgat
    85861 ctggaaatgg gcaaaattct gggggcctgt gaactcacca tcggtacgcg cctgcactcc
    85921 gccattatct caatgaactt tgccaccccg gcgattgcca tcaattatga acataaatcc
    85981 gccgggatta tgcagcagct gggactaccg gagatggcga ttgatatccg tcatttatta
    86041 gacggcagcc tgcaagtgat ggttgcggat accttaggcc agcttccggc actgaacacg
    86101 cgacttaacg aagccgtcag tcgcgaacgt cagacgggaa tgcagatggt gaaatctgtg
    86161 cttgaacaca tcagggaggc gaaatgaagg tcggcttctt tttactgaaa tttccgctgt
    86221 cgtcggaaac cttcgtcctc aatcaaatta ccgcgtttat tgatatgggc tttgaggtgg
    86281 agattgtcgc gctgcaaaaa ggcgacaccc agaacaccca cgcggcatgg acgaaataca
    86341 accttgccgc cagaacccgt tggttacagg acgaacctac gggcaaagtg gcgaaactgc
    86401 gccaccgtgc cagccagatc ttacgcggca ttcatcgtaa aaatacctgg caggcactta
    86461 acctcaaacg ctatggtgcc gagtcgcgaa acctgatttt gtctgccatt tgcggccagg
    86521 tcgcaacacc attttatgcc gatgtcttta tcgctcattt tggccctgcg ggggtaaccg
    86581 cagcaaaact ccgcgaactg ggtgtcattc gcggcaaaat tgccactatc ttccacggca
    86641 ttgatatctc cagtcgggag gtgctcaacc attacactcc cgaatatcag caactgttcc
    86701 gccgtggcga tctgatgcta ccaataagcg atctgtgggc cggaaggttg caaaaaatgg
    86761 gctgcccgag ggaaaaaatc gccgtatcgc gcatgggcgt ggacatgacg cgttttagcc
    86821 cgcgtccggt gaaagcgccc gcaacgccgc tggaaatcat ttccgtcgca cgcttaaccg
    86881 agaaaaaagg cctgcatgtg gcgattgaag cctgccgtca gttgaaagag cagggcgtgg
    86941 catttcgcta tcgcatcctc ggcattggcc cgtgggaacg acgcctgcgc accctcatcg
    87001 aacaatatca actggaagat gtggtagaga tgccgggctt taaaccgagc cacgaagtga
    87061 aagcgatgct cgacgacgcg gatgtcttcc tgttgccatc ggttacaggt gcggatggcg
    87121 atatggaagg tattccggtg gcactaatgg aagcgatggc ggtcggcatt ccggtggttt
    87181 ctactctgca tagcggaata ccggaactgg tggaggccga taaatccggc tggctggtgc
    87241 ctgagaacga tgctcgcgca ctggcgcaac gactggcggc gtttagccaa ctggacaccg
    87301 acgaactggc tccggtcgtc aaacgtgcgc gcgaaaaagt cgaacacgat tttaaccagc
    87361 aggtgatcaa tcgagaactc gccagcttgc tgcaggcttt atagaggaga acgcatgcca
    87421 tttaaaaaac tctcccgacg caccttcctg acggcaagct cggtgcttgc cttcctccat
    87481 acccctttcg ctcgcgcgct tcccgcccga caaagcgtta acattaacga ctacaaccct
    87541 cgcgactgga tcgcctcatt taaacaagcc ttcagcgaag ggcaaacggt cgtcgtgcct
    87601 gccggattgg tttgcctcaa tatcaacacc ggcatcttta tccctcctgg caaaacgtta
    87661 cacatccttg gaagcctgcg cggcaacggc agagggcgat ttgtcttaca ggacggcagc
    87721 caggtgacag gggaggaggg cggcagtatg cataacatca ccctggatgt gcgcggctct
    87781 gactgcacca tcaaagggct ggcgatgagc ggctttggtc cggtaacgca gatttatatc
    87841 ggcggcaaaa acaaacgggt catgcgtaac ctgaccatcg ataacctgac cgttagccac
    87901 gctaattacg ccatcttacg ccagggattt cataaccaga ttatcggtgc caacatcacc
    87961 aattgtaagt tcagcgactt acagggtgac gccattgaat ggaatgtggc gattaacgac
    88021 cgtgatattt tgatatctga ccatgtcatc gagcgcatca actgtactaa cggcaaaatc
    88081 aattggggca tcggcatagg tcttgcggga agcacttatg ataacaacta tccggaagac
    88141 caggcagtga aaaactttgt cgtggcgaac atcacgggat cggattgtcg gcagttgata
    88201 catgttgaaa atggtaaaca ttttgttatt cgtaatatca aagcctgcaa tatcacgccg
    88261 gatttcagta agaaagcagg tattgataac gccacggtcg ctatttacgg ttgtgacaat
    88321 ttcgtgattg ataatattga aatgattaat agcgccggga tgttaatcgg ctatggggta
    88381 attaaaggca aatatctctc gataccgcag aatttccgag tgaataatat tcagctggat
    88441 aatacccacc ttgcttacaa attgcgcggc atccaaatct ccgccgggaa tgctgtctcc
    88501 tttgtggcgc tgactaacgt tgagatgaag cgtgcgtcgc tggagttaca caacaagccg
    88561 caacaccttt ttatgcgtaa tatcaaagtg atgcaggaat cctctgttgg acccgcattg
    88621 agcatgaact tcgacatgcg caaagacgtt cgcggcgtgt ttatggcgaa aaaagaaaca
    88681 ctgctatctc ttgcaaatgt tcatgcggtg aatgaaagag ggcaaagctc cgtcgatatc
    88741 gacaggataa atcaccatat tgttaatgtg gaaaagatta actttagatt gccggaacgg
    88801 agggagtaga tttgcgacca ttcctggaaa aatggagcca tacttaggaa caatgctact
    88861 gcaatctaca acgaagcggc gtaacatcac aagtaattca gtaatcaatt cagggtaatt
    88921 gatgctggcg aaaaaaatcg aacaagctat aattcagcaa ccattttaca ggtggatgaa
    88981 ataatgacga atttaaaagc agttattcct gtagcgggtc ttgggatgca tatgttgcct
    89041 gccactaagg cgattcccaa agagatgcta ccgatcgtcg acaagccaat gattcagtac
    89101 attgttgacg agattgtggc tgcagggatc aaagaaatcc tcctggtaac tcatgcgtcc
    89161 aagaacgcgg tcgaaaacca cttcgacacc tcttatgaat tagaatctct tcttgaactg
    89221 cgcgtgaagc gtcaactgct ggcggaagta cagtccatct gtccgccagg cgtgaccatt
    89281 atgaacgtgc gtcagggcga acctttaggt ttaggccact ccattttgtg tgcacgaccc
    89341 gccattggtg acaatccatt tgtcgtggta ctgccggacg ttgtgatcga cgacgccagt
    89401 gccgacccgc tgcgctacaa ccttgctgcc atgattgcgc gtttcaatga aacgggccgt
    89461 agccaggtgc tggcaaaacg tatgccgggt gagctctctg aatactctgt catccagacc
    89521 aaagaaccgc tggatcgcga aggtaaagtc agccgcattg ttgaatttat cgaaaaaccg
    89581 gatcagccac aaacgctgga ctcagacatc atggccgttg gtcgctatgt actttctgcc
    89641 gatatttggc cggaactgga acgtactcag cctggtgcat ggggacgtat tcaactgact
    89701 gatgctattg ccgagctggc gaaaaaacag tccgttgatg ccatgctgat gactggggac
    89761 agttacgact gcggtaaaaa aatgggctat atgcaggcgt tcgtgaagta tggattacga
    89821 aacctgaaag aaggggcgaa gttccgtaaa ggcattgaga agctgttaag caaataatga
    89881 aaatctgacc ggatgtaacg gttgataaga aaattataac ggcagtgaag attcgtgatg
    89941 aaagtaattt gttgcgaata ttcctgccgt tgttttatat aaacaatcag aataacaacg
    90001 agttagcaat aggattttag tcaaagtttt ccaggatttt ccttgtttcc agagcggatt
    90061 ggtaagacaa ttagcgtttg aatttttcgg gtttagcgcg agtgggtaac gctcgtcaca
    90121 tcgtagacat gcatgcagtg cactggtagc tgtaaagcca ggggcggtag cgtgcattaa
    90181 tacccctatt aatcaaccta agagccgctt atttcacagc atgctctgaa gtaatatgga
    90241 ataataaagt gaagatactt gttactggtg gcgcaggatt tattggttct gctgtagttc
    90301 gtcacattat aaataatacg caggatagtg ttgttaatgt cgataaatta acgtacgccg
    90361 gaaacctgga atcacttgct gatgtttctg attctgaacg ctatgttttt gaacatgcgg
    90421 atatttgcga tgcagctgca atggcgcgga tttttgctca gcatcagccg gatgcagtga
    90481 tgcacctggc tgctgaaagc catgttgacc gttcaattac cggccctgcg gcatttattg
    90541 aaaccaatat tgttggtact tatgtccttt tggaagccgc tcgcaattac tggtctgctc
    90601 ttgatagcga caagaaaaat agcttccgtt ttcatcatat ttctactgac gaagtctatg
    90661 gtgatttgcc tcatcctgac gaagtaaata atacagaaga attaccctta tttactgaga
    90721 cgacagctta cgcgccaagc agtccttatt ccgcatccaa agcatctagc gatcatttag
    90781 tccgcgcgtg gaaacgtacc tatggtttac cgaccattgt gactaactgt tcgaataact
    90841 acggccctta tcactttccg gaaaaattga ttccgctggt aattcttaat gctctggaag
    90901 gtaaggcatt acctatttat ggcaaagggg atcaaattcg cgattggcta tatgttgaag
    90961 atcatgcgcg tgcgttatat accgttgtca ccgaaggtaa agcgggtgaa acttataaca
    91021 ttggcggaca caacgaaaag aaaaacatcg atgttgtgct gactatttgt gatttgttgg
    91081 atgagattgt accgaaagag caatcttatc gtgagcaaat tacttatgtt gccgatcgtc
    91141 cgggacacga tcgccgttat gcgattgatg ctgagaagat tagccgcgaa ttgggctgga
    91201 aaccgcagga aacgtttgag agcgggattc ggaagacagt ggaatggtac ctgtccaata
    91261 caaaatgggt cgaaaatgtg aaaagtggtg cctatcagtc atggattgca cagaactatg
    91321 agggccgtca gtaatgaata tcctcctttt cggcaaaaca gggcaggtag gttgggaact
    91381 acagcgtgct ctggcacctc tgggtaattt gattgctctt gatgttcact ctactgatta
    91441 ttgcggtgat tttagtaatc ctgaaggtgt agctgaaacc gtaagaagca ttcggcctga
    91501 tattattgtc aacgcagccg ctcacaccgc agtagacaaa gcagaatcag aaccggagtt
    91561 tgcacaatta cttaacgcaa caagtgtcga agcgattgcg aaagcagcca atgaagtcgg
    91621 cgcctggctt attcattact cgactgatta cgtcttccct ggaaatggcg atatgccatg
    91681 gcgggagacg gatgcaaccg caccactaaa tgtttacggt gaaaccaagt tagccggaga
    91741 aaaagcgtta caggaatatt gcgcaaagca tcttattttc cggaccagct gggtctatgc
    91801 aggaaaagga aataacttcg ccaaaacgat gttacgtctg gcaaaagagc gtgaagaatt
    91861 agcggttatt aatgatcagt ttggtgcgcc aacaggtgct gaactgctgg ctgattgtac
    91921 agcacatgcc attcgtgtcg cactgaataa accagaagtc gcaggcttgt accatctggt
    91981 agccagtggt accacaacct ggcacgatta tgctgcgctg gtttttgaag aggcacgcaa
    92041 agcaggtatt ccccttgcac tcaacaagct caacgcagta ccaacaacag cctatcctac
    92101 accagctcgt cgtccgcata actctcgcct taatacagaa aaatttcagc agaaatttgc
    92161 gcttgttttg cctgattggc aggttggcgt gaaacgaatg ctcaacgaat tatttacgac
    92221 tacagcaatt taatagtttt tgcatcttgt tcgtgatggt ggagcaagat gaattaaaag
    92281 gaatgatgaa atgaaaacgc gtaaaggtat tattttagct ggtggttcgg gtactcgtct
    92341 ttatcctgta actatggctg tcagtaaaca gttgttaccg atttatgata aaccgatgat
    92401 ctattacccg ttgtctacac tgatgttagc gggtcttcgc gatattctga ttattagtac
    92461 gccacaggat actcctcgtt ttcaacaact gctgggtgac gggagccagt gggggctaaa
    92521 tcttcagtac aaagtgcaac cgagtccaga tggtcttgcg caggcattta tcatcggtga
    92581 agagtttatc ggtggtgatg attgtgcttt ggttctaggt gataatatct tttacggtca
    92641 cgatctgccg aagttaatgg atgtcgctgt taacaaagaa agtggtgcaa cggtatttgc
    92701 ctatcacgtt aatgatcctg aacgctacgg tgtcgttgag tttgataaaa acggtacggc
    92761 gatcagcctg gaagaaaaac cgctacaacc aaaaagtaat tatgcggtaa ccgggcttta
    92821 tttttatgat aactacgttg tcgaaatggc gaaaaatctt aagccttctg cccgcggtga
    92881 actggaaatt accgatatta accgtattta tatggaacag gggcgtttat ccgttgccat
    92941 gatgggacgt ggttatgcat ggctggacac ggggacacat caaagtctta ttgaggcaag
    93001 caattttatc gcaacaatag aagaacgtca ggggctgaaa gtttcctgcc cggaagaaat
    93061 tgcttaccgt aaagggttta tcgatgctga gcaggtgaaa gtattagctg aaccgttgaa
    93121 gaaaaatgct tatggtcagt atctgctgaa aatgattaaa ggttattaat aaaatgaacg
    93181 taattaaaac agaaattcca gatgtactga tttttgaacc gaaagttttt ggtgatgagc
    93241 gtggtttctt tatggaaagc tttaatcaga aagtttttga agaagctgta gggcggaagg
    93301 ttgaatttgt tcaggataat cattctaaat catctaaggg tgtgttacgc ggactgcact
    93361 atcagctgga accctatgct caaggtaaat tagttcgttg tgtggtcggt gaagtatttg
    93421 atgtagcagt tgatattcgt aaatcgtcac ctacatttgg gaaatgggtt ggggtgaatt
    93481 tgtctgctga gaataagcgt cagttgtgga tccctgaggg gtttgcgcat ggttttttgg
    93541 tgttaagcga gatagcagag tttgtttata agacgacaaa ttattatcat cctgaatcag
    93601 aaggttgcat aaaatgggat gattcttttt tgatgattga ttggccaaat aaaccgatag
    93661 caatctcaga aaaagataaa aaaggctcat caattttgag gctaaatgac tattgatgtt
    93721 gaaaaaaaaa cttcaaaaaa taaaggaata tcattcagta ttggagttgg caataattca
    93781 gggtgcgaat gccatatttc ctgtgttggt attcccattt tttcttatta ccttagggga
    93841 aaacatcttt tcaagtattg ctgttggtga agtactagca c
//
LOCUS       DAZFJF010000005        89537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFJF010000005 DAZFJF010000000
VERSION     DAZFJF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.005"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(81..1229)
                     /gene="ycaD"
                     /locus_tag="ACHKAU_000368"
     CDS             complement(81..1229)
                     /gene="ycaD"
                     /locus_tag="ACHKAU_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5529501.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            1544..2170
                     /gene="ycaC"
                     /locus_tag="ACHKAU_000369"
     CDS             1544..2170
                     /gene="ycaC"
                     /locus_tag="ACHKAU_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO5529502.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(2206..3069)
                     /gene="dmsC"
                     /locus_tag="ACHKAU_000370"
     CDS             complement(2206..3069)
                     /gene="dmsC"
                     /locus_tag="ACHKAU_000370"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO5529503.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(3071..3688)
                     /gene="dmsB"
                     /locus_tag="ACHKAU_000371"
     CDS             complement(3071..3688)
                     /gene="dmsB"
                     /locus_tag="ACHKAU_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO5529504.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(3699..6143)
                     /gene="dmsA"
                     /locus_tag="ACHKAU_000372"
     CDS             complement(3699..6143)
                     /gene="dmsA"
                     /locus_tag="ACHKAU_000372"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO5529505.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(6382..7674)
                     /gene="serS"
                     /locus_tag="ACHKAU_000373"
     CDS             complement(6382..7674)
                     /gene="serS"
                     /locus_tag="ACHKAU_000373"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO5529506.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(7765..9108)
                     /gene="rarA"
                     /locus_tag="ACHKAU_000374"
                     /gene_synonym="mgsA"
     CDS             complement(7765..9108)
                     /gene="rarA"
                     /locus_tag="ACHKAU_000374"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO5529507.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            complement(9119..9730)
                     /gene="lolA"
                     /locus_tag="ACHKAU_000375"
     CDS             complement(9119..9730)
                     /gene="lolA"
                     /locus_tag="ACHKAU_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO5529508.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            complement(9885..13991)
                     /gene="ftsK"
                     /locus_tag="ACHKAU_000376"
     CDS             complement(9885..13991)
                     /gene="ftsK"
                     /locus_tag="ACHKAU_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO5529509.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            complement(14126..14620)
                     /gene="lrp"
                     /locus_tag="ACHKAU_000377"
     CDS             complement(14126..14620)
                     /gene="lrp"
                     /locus_tag="ACHKAU_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO5529510.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            15164..16129
                     /gene="trxB"
                     /locus_tag="ACHKAU_000378"
     CDS             15164..16129
                     /gene="trxB"
                     /locus_tag="ACHKAU_000378"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO5529511.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16252..18018
                     /gene="cydD"
                     /locus_tag="ACHKAU_000379"
     CDS             16252..18018
                     /gene="cydD"
                     /locus_tag="ACHKAU_000379"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO5529512.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            18019..19740
                     /gene="cydC"
                     /locus_tag="ACHKAU_000380"
     CDS             18019..19740
                     /gene="cydC"
                     /locus_tag="ACHKAU_000380"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO5529513.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            19782..20486
                     /gene="aat"
                     /locus_tag="ACHKAU_000381"
     CDS             19782..20486
                     /gene="aat"
                     /locus_tag="ACHKAU_000381"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO5529514.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            20771..20989
                     /gene="infA"
                     /locus_tag="ACHKAU_000382"
     CDS             20771..20989
                     /gene="infA"
                     /locus_tag="ACHKAU_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO5529515.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            21243..21330
                     /locus_tag="ACHKAU_000383"
     tRNA            21243..21330
                     /locus_tag="ACHKAU_000383"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21277..21279,aa:Ser,seq:gga)
     gene            complement(21673..23949)
                     /gene="clpA"
                     /locus_tag="ACHKAU_000384"
     CDS             complement(21673..23949)
                     /gene="clpA"
                     /locus_tag="ACHKAU_000384"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO5529516.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(23980..24300)
                     /gene="clpS"
                     /locus_tag="ACHKAU_000385"
     CDS             complement(23980..24300)
                     /gene="clpS"
                     /locus_tag="ACHKAU_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO5529517.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            complement(24399..24473)
                     /gene="yljB"
                     /locus_tag="ACHKAU_000386"
     CDS             complement(24399..24473)
                     /gene="yljB"
                     /locus_tag="ACHKAU_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO5529518.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            24623..24847
                     /gene="cspD"
                     /locus_tag="ACHKAU_000387"
     CDS             24623..24847
                     /gene="cspD"
                     /locus_tag="ACHKAU_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO5529519.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(24920..26866)
                     /gene="macB"
                     /locus_tag="ACHKAU_000388"
     CDS             complement(24920..26866)
                     /gene="macB"
                     /locus_tag="ACHKAU_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO5529520.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(26863..27978)
                     /gene="macA"
                     /locus_tag="ACHKAU_000389"
     CDS             complement(26863..27978)
                     /gene="macA"
                     /locus_tag="ACHKAU_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO5529521.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            28129..29085
                     /gene="ybjX"
                     /locus_tag="ACHKAU_000390"
     CDS             28129..29085
                     /gene="ybjX"
                     /locus_tag="ACHKAU_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO5529522.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(29082..30740)
                     /gene="ybjD"
                     /locus_tag="ACHKAU_000391"
     CDS             complement(29082..30740)
                     /gene="ybjD"
                     /locus_tag="ACHKAU_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO5529523.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            31166..31861
                     /gene="aqpZ"
                     /locus_tag="ACHKAU_000392"
     CDS             31166..31861
                     /gene="aqpZ"
                     /locus_tag="ACHKAU_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO5529524.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            32356..33255
                     /gene="lysO"
                     /locus_tag="ACHKAU_000393"
     CDS             32356..33255
                     /gene="lysO"
                     /locus_tag="ACHKAU_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO5529525.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            33399..35051
                     /gene="hcp"
                     /locus_tag="ACHKAU_000394"
                     /gene_synonym="priS"
     CDS             33399..35051
                     /gene="hcp"
                     /locus_tag="ACHKAU_000394"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO5529526.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            35063..36031
                     /gene="hcr"
                     /locus_tag="ACHKAU_000395"
     CDS             35063..36031
                     /gene="hcr"
                     /locus_tag="ACHKAU_000395"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO5529527.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            36164..37882
                     /gene="poxB"
                     /locus_tag="ACHKAU_000396"
     CDS             36164..37882
                     /gene="poxB"
                     /locus_tag="ACHKAU_000396"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO5529528.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            37919..38920
                     /gene="ltaE"
                     /locus_tag="ACHKAU_000397"
     CDS             37919..38920
                     /gene="ltaE"
                     /locus_tag="ACHKAU_000397"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO5529529.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            38931..40361
                     /gene="ybjT"
                     /locus_tag="ACHKAU_000398"
     CDS             38931..40361
                     /gene="ybjT"
                     /locus_tag="ACHKAU_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO5529530.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            40460..41473
                     /gene="ybjS"
                     /locus_tag="ACHKAU_000399"
     CDS             40460..41473
                     /gene="ybjS"
                     /locus_tag="ACHKAU_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5529531.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(41470..42300)
                     /gene="amiD"
                     /locus_tag="ACHKAU_000400"
     CDS             complement(41470..42300)
                     /gene="amiD"
                     /locus_tag="ACHKAU_000400"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO5529532.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(42297..42620)
                     /gene="ybjQ"
                     /locus_tag="ACHKAU_000401"
     CDS             complement(42297..42620)
                     /gene="ybjQ"
                     /locus_tag="ACHKAU_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO5529533.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            42746..43261
                     /gene="ybjP"
                     /locus_tag="ACHKAU_000402"
     CDS             42746..43261
                     /gene="ybjP"
                     /locus_tag="ACHKAU_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5529534.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            43479..44207
                     /gene="artP"
                     /locus_tag="ACHKAU_000403"
     CDS             43479..44207
                     /gene="artP"
                     /locus_tag="ACHKAU_000403"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO5529535.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            44225..44956
                     /gene="artI"
                     /locus_tag="ACHKAU_000404"
     CDS             44225..44956
                     /gene="artI"
                     /locus_tag="ACHKAU_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO5529536.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            44963..45679
                     /gene="artQ"
                     /locus_tag="ACHKAU_000405"
     CDS             44963..45679
                     /gene="artQ"
                     /locus_tag="ACHKAU_000405"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO5529537.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            45679..46347
                     /gene="artM"
                     /locus_tag="ACHKAU_000406"
     CDS             45679..46347
                     /gene="artM"
                     /locus_tag="ACHKAU_000406"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO5529538.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            46639..47370
                     /gene="artJ"
                     /locus_tag="ACHKAU_000407"
     CDS             46639..47370
                     /gene="artJ"
                     /locus_tag="ACHKAU_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO5529539.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(47545..48672)
                     /gene="rlmC"
                     /locus_tag="ACHKAU_000408"
     CDS             complement(47545..48672)
                     /gene="rlmC"
                     /locus_tag="ACHKAU_000408"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO5529540.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(48713..49201)
                     /gene="ybjO"
                     /locus_tag="ACHKAU_000409"
     CDS             complement(48713..49201)
                     /gene="ybjO"
                     /locus_tag="ACHKAU_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO5529541.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(49261..50106)
                     /gene="potI"
                     /locus_tag="ACHKAU_000410"
     CDS             complement(49261..50106)
                     /gene="potI"
                     /locus_tag="ACHKAU_000410"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO5529542.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(50103..51056)
                     /gene="potH"
                     /locus_tag="ACHKAU_000411"
     CDS             complement(50103..51056)
                     /gene="potH"
                     /locus_tag="ACHKAU_000411"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO5529543.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(51066..52199)
                     /gene="potG"
                     /locus_tag="ACHKAU_000412"
     CDS             complement(51066..52199)
                     /gene="potG"
                     /locus_tag="ACHKAU_000412"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO5529544.1"
                     /translation="MNDAIPRPQAKTRKALMPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(52294..53406)
                     /gene="potF"
                     /locus_tag="ACHKAU_000413"
     CDS             complement(52294..53406)
                     /gene="potF"
                     /locus_tag="ACHKAU_000413"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO5529545.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(53757..54233)
                     /gene="ybjN"
                     /locus_tag="ACHKAU_000414"
     CDS             complement(53757..54233)
                     /gene="ybjN"
                     /locus_tag="ACHKAU_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO5529546.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(54321..55223)
                     /gene="rimK"
                     /locus_tag="ACHKAU_000415"
     CDS             complement(54321..55223)
                     /gene="rimK"
                     /locus_tag="ACHKAU_000415"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO5529547.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(55284..56006)
                     /gene="nfsA"
                     /locus_tag="ACHKAU_000416"
     CDS             complement(55284..56006)
                     /gene="nfsA"
                     /locus_tag="ACHKAU_000416"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO5529548.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(55990..56277)
                     /gene="ybjC"
                     /locus_tag="ACHKAU_000417"
     CDS             complement(55990..56277)
                     /gene="ybjC"
                     /locus_tag="ACHKAU_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO5529549.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            56437..56694
                     /gene="grxA"
                     /locus_tag="ACHKAU_000418"
     CDS             56437..56694
                     /gene="grxA"
                     /locus_tag="ACHKAU_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO5529550.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(56724..57101)
                     /gene="ybjM"
                     /locus_tag="ACHKAU_000419"
     CDS             complement(56724..57101)
                     /gene="ybjM"
                     /locus_tag="ACHKAU_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO5529551.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            57371..59056
                     /gene="ybjL"
                     /locus_tag="ACHKAU_000420"
     CDS             57371..59056
                     /gene="ybjL"
                     /locus_tag="ACHKAU_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO5529552.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(59231..59767)
                     /gene="rcdA"
                     /locus_tag="ACHKAU_000421"
     CDS             complement(59231..59767)
                     /gene="rcdA"
                     /locus_tag="ACHKAU_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO5529553.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            59851..61059
                     /gene="ybjJ"
                     /locus_tag="ACHKAU_000422"
     CDS             59851..61059
                     /gene="ybjJ"
                     /locus_tag="ACHKAU_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5529554.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            61059..61874
                     /gene="ybjI"
                     /locus_tag="ACHKAU_000423"
     CDS             61059..61874
                     /gene="ybjI"
                     /locus_tag="ACHKAU_000423"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO5529555.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            61960..62244
                     /gene="ybjH"
                     /locus_tag="ACHKAU_000424"
     CDS             61960..62244
                     /gene="ybjH"
                     /locus_tag="ACHKAU_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO5529556.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(62285..63517)
                     /gene="mdfA"
                     /locus_tag="ACHKAU_000425"
     CDS             complement(62285..63517)
                     /gene="mdfA"
                     /locus_tag="ACHKAU_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO5529557.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            63802..64398
                     /gene="ybjG"
                     /locus_tag="ACHKAU_000426"
     CDS             63802..64398
                     /gene="ybjG"
                     /locus_tag="ACHKAU_000426"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5529558.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            64456..65214
                     /gene="deoR"
                     /locus_tag="ACHKAU_000427"
     CDS             64456..65214
                     /gene="deoR"
                     /locus_tag="ACHKAU_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO5529559.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(65261..66463)
                     /gene="dacC"
                     /locus_tag="ACHKAU_000428"
     CDS             complement(65261..66463)
                     /gene="dacC"
                     /locus_tag="ACHKAU_000428"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5529560.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            66710..67336
                     /gene="gstB"
                     /locus_tag="ACHKAU_000429"
     CDS             66710..67336
                     /gene="gstB"
                     /locus_tag="ACHKAU_000429"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO5529561.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(67333..68448)
                     /gene="yliI"
                     /locus_tag="ACHKAU_000430"
     CDS             complement(67333..68448)
                     /gene="yliI"
                     /locus_tag="ACHKAU_000430"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO5529562.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(68559..68942)
                     /gene="bssR"
                     /locus_tag="ACHKAU_000431"
     CDS             complement(68559..68942)
                     /gene="bssR"
                     /locus_tag="ACHKAU_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO5529563.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            69155..70480
                     /gene="rimO"
                     /locus_tag="ACHKAU_000432"
     CDS             69155..70480
                     /gene="rimO"
                     /locus_tag="ACHKAU_000432"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO5529564.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(70527..71855)
                     /gene="dgcI"
                     /locus_tag="ACHKAU_000433"
     CDS             complement(70527..71855)
                     /gene="dgcI"
                     /locus_tag="ACHKAU_000433"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5529565.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(71863..74211)
                     /gene="pdeI"
                     /locus_tag="ACHKAU_000434"
     CDS             complement(71863..74211)
                     /gene="pdeI"
                     /locus_tag="ACHKAU_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5529566.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVLERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(74389..75300)
                     /gene="gsiD"
                     /locus_tag="ACHKAU_000435"
     CDS             complement(74389..75300)
                     /gene="gsiD"
                     /locus_tag="ACHKAU_000435"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO5529567.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(75303..76223)
                     /gene="gsiC"
                     /locus_tag="ACHKAU_000436"
     CDS             complement(75303..76223)
                     /gene="gsiC"
                     /locus_tag="ACHKAU_000436"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO5529568.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(76241..77779)
                     /gene="gsiB"
                     /locus_tag="ACHKAU_000437"
     CDS             complement(76241..77779)
                     /gene="gsiB"
                     /locus_tag="ACHKAU_000437"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO5529569.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(77799..79670)
                     /gene="gsiA"
                     /locus_tag="ACHKAU_000438"
     CDS             complement(77799..79670)
                     /gene="gsiA"
                     /locus_tag="ACHKAU_000438"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO5529570.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(79657..80622)
                     /gene="iaaA"
                     /locus_tag="ACHKAU_000439"
     CDS             complement(79657..80622)
                     /gene="iaaA"
                     /locus_tag="ACHKAU_000439"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5529571.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            80826..82061
                     /gene="moeA"
                     /locus_tag="ACHKAU_000440"
     CDS             80826..82061
                     /gene="moeA"
                     /locus_tag="ACHKAU_000440"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO5529572.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            82061..82810
                     /gene="moeB"
                     /locus_tag="ACHKAU_000441"
     CDS             82061..82810
                     /gene="moeB"
                     /locus_tag="ACHKAU_000441"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO5529573.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(82886..83548)
                     /gene="fsa"
                     /locus_tag="ACHKAU_000442"
     CDS             complement(82886..83548)
                     /gene="fsa"
                     /locus_tag="ACHKAU_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5529574.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            83679..84578
                     /gene="ybiY"
                     /locus_tag="ACHKAU_000443"
     CDS             83679..84578
                     /gene="ybiY"
                     /locus_tag="ACHKAU_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO5529575.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            84584..87016
                     /gene="ybiW"
                     /locus_tag="ACHKAU_000444"
     CDS             84584..87016
                     /gene="ybiW"
                     /locus_tag="ACHKAU_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO5529576.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            87162..87977
                     /gene="ybiV"
                     /locus_tag="ACHKAU_000445"
     CDS             87162..87977
                     /gene="ybiV"
                     /locus_tag="ACHKAU_000445"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO5529577.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            88129..89394
                     /gene="ybiU"
                     /locus_tag="ACHKAU_000446"
     CDS             88129..89394
                     /gene="ybiU"
                     /locus_tag="ACHKAU_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO5529578.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT        21651 a        23787 c        22313 g        21786 t
ORIGIN      
        1 aatttggaat aaagcaagtc agatagaaga aaaaaaaagc agagtgtgaa acagtaagtt
       61 cacactctgc ttgaattcat ttacacgtga gcaacgggtt tcggcgtatg accggcgttg
      121 cgcagcagca tcagcaaata gataaacgat acgctggcga tcatgataaa caataaatta
      181 tcggagaaat tctgcattag catagcggta aatgacgggc caagcagact tcccacagta
      241 tagctcaaca gtaaggcctg gttcatcgcc accagttgat gatgttcaac tttctcgcaa
      301 gcccatgcca tcgccaccgg atatagcgta aagccagcgg caccgaggat gaataacgct
      361 ggggccatcg ccgcctggct aagcatcgcg atactgccga gaatgacgac aaagacctga
      421 acacgcaaca ccagcagtcg accaaactta tccgccagac gtccaatcgg ccattgtcca
      481 aggatacccg cactgaccag taccgccatc cagaaaccaa tgctggcatt gctcacccct
      541 ttgtgattga ggtacagcgg catcaggcca tatagagatc ccagaacgat tcctgagata
      601 atgcagccat tcacgccaag ccgcgcctga cggagtttta gcattgacgt aattgacgtc
      661 gaatcatggt tttcagcctg ctgattcagc acacgcgtaa acaacagcgg taagatccct
      721 gccaacgtca aacctgtaac ccacggcaat acggacatca gctcggttga aactttgctg
      781 accagtaact ggcctaaaaa cgttcccacg taataaacca tcatatacgc agcaagcaaa
      841 cgcccacggt tacgtgacgt cccactgcac atcagcgcgc tctcaacaac cacccaaatc
      901 atggcacagc cgacgcccgc gacaaaacgc caagccaacc agctccagaa tccaatcatc
      961 aggccaaggc cggcacagcc agcggcaaaa atgaaggagg ccagataata gctgcggtta
     1021 aagccaatgc gcttaatgac ataccctgtc agcaatgtac cgacaaggtt gccggtaaaa
     1081 taggatgagc tgacaacgcc tacctgccat gtggacatgt gttcctgggc gagccaaagc
     1141 ggcacgagtg tatttaacac cgcgatcgcc agagtcaaca aaagcaggcc agacagcaaa
     1201 agcatgacag gccgggtata cgtggacatg aattaaaacc gtgaggaagt tcaaatttca
     1261 tgcgcatcat gccaccgcca aaaacattgt caatgggtga aaatacgcgc atatcgcagc
     1321 ggcggtatta tcgatttata ttactcattc ataacgctaa tgctgaagag atgattaatc
     1381 tcatctgatt gtttttattg atctaacaca aatttcatac gcttcgttca gtcgaaaaat
     1441 ttcatggtca cagcaaactc atatttcccg tctatgcttt cagagcaatg catcacgtct
     1501 gaccgatttc agacgtacca ctgactctcg aaaggagaag aggatgacca aaccgtatgt
     1561 tcgtcttgat aaaaatgatg ctgccgtttt gcttgttgat catcaggctg gtttactttc
     1621 ccttgtacgg gatatcgaac ccgataagtt taaaaataac gtgctggcgc tgggtgacct
     1681 ggctaagtat ttcaacttac ctactattct taccaccagt tttgaaaccg gtcctaacgg
     1741 tccattagtt ccagaactga aagcacaatt ccccgatgca ccttacattg ctcgcccggg
     1801 aaatattaac gcctgggata acgaagattt tgtaaaagca gtcaaagcga caggtaaaaa
     1861 acagttaatt attgccggtg tggtaaccga agtttgcgtg gcattcccgg cgctttcagc
     1921 cattgaagaa gggtttgatg tctttgttgt gactgacgcg tcagggacct ttaatgaaat
     1981 tacccggcat tcggcatggg atcgcatgtc gcaagcaggt gcgcaattga tgacatggtt
     2041 cggcgtggcg tgcgagctgc accgtgactg gcgtaatgat attgaaggac tggcgacgtt
     2101 gttctctaac catattcctg attatcgtaa tttgatgacc agttatgaca cgttaacgaa
     2161 gcagaaataa aagagaaccg gggccgaaac cccggttgtt actgattagc ttgcgacggc
     2221 catccccacg gtcatatgca aaccatagaa tacgccgcga ccgattaatt cccctgccag
     2281 aagcagaatg aatgagacgg atagtagcgg aaccgcaggc tgataacctt ttagctgtgg
     2341 tgcaatccac aagcacaggg caacggccaa aagcacgatc cgccaggaca tcagcgcacc
     2401 atagtccggc accagcgctg cggcctgctg cacagaacta tgaatagttg ccagctctgt
     2461 gccctgcatc actgacatca cgccactcac caccagtgcc agtacagaaa tggctggcag
     2521 cagacgcatc gcccagccat cgacgccagc cagactcaac aacaggtaac ccagtaacgg
     2581 gccgcccata aacatcgtca ggaagaagcc catcggcgtc cagatgctgt accaggtcgg
     2641 aacggtatca atgctgttat acacacgcac catcatccag acaaagatga cgccaagaac
     2701 catcgtcact atcagccaca gcgtacgcaa tgccggtgac agctttttca gcattgccag
     2761 caaccagccg atgccgccta cggcaaaaaa gatcgaaccg ctggcgattt cgttactgag
     2821 tgctgaagcc cctacccggt tgagcgagtt aaaagcgcgc attggtgaac caagatggag
     2881 catagaggcg ataaagccaa tgcccatcag cacccataaa ccaaacatgc aggcgataac
     2941 acgctgctgg gcttctgctc gcaggtcgcc tttgagcagc gccaaagcca gaacgataaa
     3001 accacctgct acacattgcc cgaagaccgt gaagatcatc agcggccatt catgccatcc
     3061 acttcccatc tcacacctcc ttcgggtttg ccagatagcc agtggtatcc ccggtcgggc
     3121 ggctattggc attgggtttg atcacaatat tcggtttggt aaagtgagct cgcggcaacg
     3181 gcgcaacggc cgccagatcg ccatgttttt tacgcagctc gtcgataggg ccgaaatcca
     3241 gcgcccgcag cggacaagat tcaacacaga tcggcttttt accctccgca acacggtcat
     3301 aacaaccatc gcatttggtc atatggcctt tcgtttcgtt atattgcggt gcgccatacg
     3361 ggcaagccat atggcagtag cggcagccaa tgcacacatc ttcatcgacc acaacaaaac
     3421 catcttcacg tttatgcatc gcaccgctcg ggcagacttt agtacaagcc ggatcttcgc
     3481 agtggttaca tgaaatcgac agatagtagg caaacacgtt ctggtgccag acaccgttat
     3541 cttcctgcca gtcgccgcca gcatattcat aaatgcggcg gaagctgact tctggcgtca
     3601 aatctttgta gtctttacag gccagctcgc aggttttgca accggtgcaa cggctggaat
     3661 caataaaaaa tccatactgg gttgtcatcg gttactcctt acaccttttc aacctgaaca
     3721 aggtttgtat gtgacggatt ccccttagcg agaggagacg gacgttgagt ggtcagtacg
     3781 ttgatacaac cgcccttatc gacacgtttt gtatccgggt cataccaggc accttcaccc
     3841 agtgcgacca cacccggcat cattcgtggc gtcactttcg cctcaatatg aacctcacca
     3901 cgatcgttaa agatcctgac tttatcgccg ttgttgatac cgcgtttttg cgcatcaagc
     3961 gggttgatcc acatttcctg acggcaagct gctttcagca catcaacgtt gccgtaagtt
     4021 gagtgaacgc gagatttata gtggaaaccc gtaagctgca gcggatactg tttgttcagc
     4081 ggatcctgat aactttcaaa gcccggcgtg tagatcggca gtggatcgat cacatcgcct
     4141 tctggcaatt cccaggtagc ggcaatgtca gccagcgcct gcgaataaat ctcaattttg
     4201 cccgatggcg tagtcagtgg gtttgcctgc ggatcttcac ggaaggcttt ataagcaacg
     4261 tgatgccctt gtgggtcgcg ctttttaaag atcccctgct tgcggaactc ttcaaacgtt
     4321 ggcagttcag gaatcgcttc ccgcgactgg gcatacagat gccgcatcca ctcttcctgg
     4381 gtacggcctt cagtaaactg ttgctcaacg ccaagacgtt ttgccagttc gctggtcatt
     4441 tcatagatgg tcttacattc aaagcgcggt ttaatcacct gatcgttgaa aatcacgtaa
     4501 gacatattcc cgcaggatgc atccagtgca aagtccatct gttcggaagc ggtgcagtca
     4561 ggcagcagga tgtcagcata tttcgccgat gaggtcatgt ggcagtcgat aaccacaatc
     4621 agctcgcact tcttatcatc ctgaaggatt tcatgggtac ggttgatttc agaatgctgg
     4681 ttaatcaggc agttaccggc atagttccag atcattttga tcggcacatc cagcttatct
     4741 ttcccgcgta caccatcacg cagcgccgtc atttccgggc cacgttcaat ggcatcggtc
     4801 cacataaaca tcgaaatgct ggtctggatc gggttttcca aggtcggcat acggacaaac
     4861 ggtaagctgt atgaaccttc acgcgcgccg ctgttgcctc cgttaatacc aacgttaccg
     4921 gtcagaatcg ccagcatcga gatagcacgg gttgcgattt caccgttagc gtgacgctgc
     4981 gggccccatc cctggctgat aaacgccggt ttggtactgc cgatttcacg agccagtttg
     5041 atgattttgt ctgccggaac accggtgatt tgtgaggccc attccggtgt tttagccacg
     5101 ccatctggcc cttcacccag aatataagct ttatagtggc catttttcgg cgcactggct
     5161 ggcagggttt tctcatcgta gccaacgcaa tatttatcga ggaatgcctg atccaccagg
     5221 ttttcagtga tcatgacgta cgccagaccg ttaaccagtg cggcatctgt tcccggacga
     5281 atagggatcc actcatcttc gcgcccggca ccggtgtcgg tatagcgcgg atcaatgatg
     5341 atcatgcggg cattagattt ctggcgtgcc tgttcaagat agtaagtcac cccaccgcca
     5401 ctcattcgcg tttcgccagg gttattacca aacagcacta ccagcttact gttttcgata
     5461 tccgacgggc tgttgccatc tgcccagccg ccgtaggtat agttcaaacc ttccgcaatt
     5521 tgcgcggagg agtagtcgcc gtaatggttc agatagccgc cgcagcagtt catcagccgc
     5581 gcgaccaggg tatttcccgg cggccaggag cgggtcatgg tgccgcccag cgtaccggtg
     5641 ccatagttca gatagataga ctcgttgccg tactctttga tcaggcgctg catattggtc
     5701 gcgatgatgt cgtaggcttc ttcccagcta atgcgctcga atttgccttc accgcgcgcc
     5761 ccgactcgtt tcatcggata tttcaggcgg tccggattgt agacacggcg acgcatggaa
     5821 cgcccacgca ggcaggcgcg aacctggtgc aggccgtcgt aattgtcatc gccggtattg
     5881 tccgtttcga catatttgat ttcaccgtcc acgacgtgca tacgtagcgg gcagcgacta
     5941 ccacagttaa ctgtacaggc gctccagata accttttcgt ctgattttgt tggaatggcg
     6001 ctatcgacag cgtgcgcaat ccgactaaaa ggtaatgtta atgcgctgct ggccattgcc
     6061 aggccgccga tcgctgtcgt ttttaccaaa ccacggcgac tcacctcagc agccaatacc
     6121 gcatcaggga ttttcgtttt cataatggct cactcaagct tgctcacgat tgattccagt
     6181 aacaaaccgt attgtgtaat tatttctata gcagctcgct atataggctt gtatacatcg
     6241 aataatttga aaatcaaaga gataggtaga ttttcactcg aaagtagtat tacgtgtaag
     6301 tgaggagtaa ttattgttcg gtatcaaagg gagacaaaaa aagcgcccgc aggcgctttt
     6361 tagattcaga aaaattgggt attagccaat atattccagt ccgttcatat acggacgcag
     6421 aacttctggt acttcaatac gaccatcagc ctgctgatag ttttccatta ctgcaaccag
     6481 cgtacgacca acagccagtc cagaaccgtt cagggtatga accagacggg ttttcttgtc
     6541 cgacttgctg cggcagcgtg cctgcatacg acgcgcctgg aaatcccaaa cgttggagca
     6601 ggaagagatc tcacggtagg tgttctgtgc cgggatccat acttccaggt cgtaagtttt
     6661 gcaagcgcca aagcccatgt cgccagtgca aaggatgatt ttacggtacg gcaggcccaa
     6721 caactgcagg actttttctg catgaccagt catctcttcc agcgccgcca ttgagtcttc
     6781 cgggcgcacg atctgcacca tttcaacttt gtcgaactgg tgcatacgga tcagaccacg
     6841 ggtgtcacga ccatatgaac cggcttcaga acggaagcat ggggtgtggg cggtcatctt
     6901 aattggcaga tcatcttcat cgatgatttc accgcgcacc aggttagtca gcggaacttc
     6961 tgccgttggg atcagcgcat agttactggt gtctgcttct tcttccagcg gacgagtatg
     7021 aaacagatcg ccagcaaatt tcggcagctg acccgtaccg tacagcgtgt cctggttaac
     7081 caggtacgga acatagttct cactgtagcc atgctgttcg gtatgcagat ccagcataaa
     7141 ctgcgacagt gcgcggtgca tgcgagcaat ctgccctttc attaccacaa agcgggaacc
     7201 agtcagctta actgcagctg caaagtcgag gccagagtgc atttcaccca gcgtcacatg
     7261 gtcacgaact tcaaagtcaa actcacgcgg ggtgccccag cggctgactt cgacgttgtc
     7321 attttcgtct ttacctaccg gcacttcatc tgcaggcagg ttagggatgg tcagcgcgat
     7381 atcgcgaatt tcagcctgta aagcatccag ctcggctttt gctgcatcca gctcttcgcc
     7441 cagtttgttc acttccagac gtaaaggctc gatatcttcc ccgcgcgctt tcgcctggcc
     7501 aatggatttc gatcgggagt tacgctccgc ttgcaggttt tccgttttga cctgcaatac
     7561 tttacgacgc tcttcaagag cgcccagctt atctacatcc agcttaaagc cccggcgtgc
     7621 cagtttttca gcgactgcgt ctggctcatt acgcagcaga ttgggatcga gcatgcttat
     7681 cctgtgctta tcgaattaaa tgggagatgt ggccacagcc tgcgaccaca gggatacagt
     7741 aacaacatta ccgcaacgat aacattaacg gtagcgtttt atggggctat tttgatcctg
     7801 ttcagccagc caggcgagct tttcgccaat cttgccttca aggcccctgt ttgtcgggaa
     7861 ataatagcgt gtttgtgcta tttccggcgg gaagtaaacc tcaccggcag cataagcgtt
     7921 tgcttcatca tgagcgtaac gatattcctg cccgtagccc atttccttca ttaatttcgt
     7981 cggcgcatta cgcaaatgaa ccggcacgtc ataatccgga cgttcgcgag catccgccag
     8041 cgcagcttta aacgcagtgt agacagcgtt gctttttggc gcgcaggcca ggtaaacaat
     8101 cgcctgagca atggcgcgtt caccttccgc cgggccaacg cgagtaaagc aatcccaggc
     8161 cgcaattgcc acctgcatcg cccgtgggtc ggcattgccg acatcttcgg aagcaattgc
     8221 caggcaacga cgcgcgacat ataacggatc gccaccagcg gtaataattc gcgcatacca
     8281 gtagagcgcc gcatcgggtg cgctaccacg taccgactta tgcagtgcgg aaatgagatc
     8341 gtaaaagcga tcgcctttgt tatcaaagcg ggcgcttcgc tcaccggcga tttcggtcaa
     8401 taattcaggc ttcaggaccc gcttaccgct atcattgact tcggccatat ccgccatcat
     8461 ttccagcgta tttaacgccc ggcgcgcgtc gccattcacc agttcagcaa tggcgcgtcg
     8521 tgtttcatct ggcagaacaa tatcctgacc accataacca cgggtcttgt cttccatcgc
     8581 ctgagttagt acttgctcaa tatcctctgt acccagggat ttcaacagat agacacgggc
     8641 acgggaaagc agtgccgaat taagctcaaa cgacgggttt tcagtggttg cgccaataaa
     8701 agtgatggtg ccgtcttcaa tatgtggcag aaatgcatcc tgctggcttt tgttgaatcg
     8761 gtgcacttcg tcaacaaaaa gaatagtgcg gcgacctgca ttgcggtttt gccgggcgcg
     8821 ctcgatcgcc tcgcgaatct ctttcacgcc agaggtgacg gcagaaatac gttccacatc
     8881 agcgttcgca tagcgggcaa tcacttcagc gagggttgtt ttgccggtac ccggtggccc
     8941 ccagaggatc atagaatgta aatgcccggc ttcgatagcg cgcggcaacg gcttccccgc
     9001 agccagcaaa tgttgctggc cgatatactg tgctaaattt tctggccgca tacgcgcggc
     9061 cagaggttga aaagtattat ccgaaaaatc gagcgacaga ttgctcactc aggtgcctct
     9121 acttacgttg atcatctacc gtgacgcctt gcggcggggt gaaggtaaat ttcgctgcat
     9181 ccacagcccc attttgctgg gatttcagtt gataactgct gcgctgatcg tcctgctcca
     9241 ccgcgctaaa ctgatggatt gtgccatcac gtcccacgtt aatggtgaac tgcttcagat
     9301 tgccattgct ggctttcggc gtcaggacaa agtcatcgcc attctgtttg atattgtact
     9361 gctgccagtc gctggactgg ttgcgggcaa tcagcataaa cggcgtatta ccggtggcat
     9421 ctttcagcca ggttgccgta gcttgctcaa cgaacgggtt atagaaccac agtgttttac
     9481 cgtcagaaac cagaatgctt tcatcaggtt gtgtcatatg ccagttgaat aagtttggac
     9541 gtttcaccca cagatcgccc tgaccttcct gcaccgccgc gccgctaccg tcagtcactt
     9601 tttgtgtgaa gctggcgtgg aagctgctga ctttatccag gcggcttttc agatcgcttg
     9661 cggcatcagc ccaaacgctg cttgctacta agcttgagag taatgcacag gtgatggcaa
     9721 tttttttcat cattattcct caaattacgt cactcccgat cgggagttcc ctctactctc
     9781 tattccaggc taataatcag cttgaggaaa gaagaaaata ctgaattttc agttgataga
     9841 gtgccggatg cgacgctacc gcgccttatc cggcaatgca ttaattagtc aaacggcggt
     9901 ggggccagca cttcacgatt accgttgtgc ccctgttcgc tgacaatccc ctgcgcttcc
     9961 atctgttcga taatacgcgc tgcacggtta taaccaatgc ggaactgacg ctgtacgcca
    10021 gaaattgacg ctttgcgttt ttcagtgaca aactgcaccg cctgatcgaa caacggatcc
    10081 agttcttcag cgccatcgaa accgcccgcg ccaccttcgc tttcactgtc ggaagtgatg
    10141 ccatcaacat actgtgggcg accacgcgct ttccagtcct gcaccacggc atgaacttcc
    10201 tgatcgcgaa caaaagcacc atggacacgt accggcaacg tggagttcgg cccagagtag
    10261 agcatatccc ccatccccag cagtgattcc gcgccagcct gatcaagaat ggtacgtgag
    10321 tcaatcttac tggatacggt aaaggcgata cgggtcggaa tattcgcttt aatcagacca
    10381 gtaataacat caaccgatgg acgctgagtt gccagtacga ggtggatacc cgcggcacgg
    10441 gctttttgcg ccagacgtgc tatcagctct tccacttttt tacctaccgt catcatcagg
    10501 tcggcaaatt cgtcaaccaa caccacaatg tatggttctt ttttcagcac cggatgctgg
    10561 gcatccatac tgtcacccgg cttccagtac gggtctggaa tcggacgcat catgcgatcg
    10621 gcttcagcaa ttttttcgtt ataacccgcc agattacgca cacccagcgc agacatcagt
    10681 ttataccgac gctccatctc gttaacacac cagcgcagcg cgttggcggc atctttcata
    10741 tcagtaacga cttccgttaa cagatgcgga atgccttcat aaaccgaaag ctccagcatt
    10801 ttcgggtcga tcatgatgaa acgcacatct tctggctgtg ctttataaag catgctcagg
    10861 atcatcgcgt tcacaccgac agatttaccg gaaccggtag tccccgcaac caacaagtgc
    10921 ggcattttcg ccagatcggc aaccaccggc tcaccggcga tatctttacc cagcaccacg
    10981 gttaatggcg acggattatc gcggaatttg gcgttatcca aaacttcgcg cagataaacg
    11041 gtttgtcgtt ttttatttgg taactccaga cctacatagg gtttgccagg aataacttca
    11101 acgacacgca ccgccaccgt cgaaagtgaa cgggcaaggt cccgtgacaa gttagaaatg
    11161 cgcgccgctt ttacgccagg agccaggtta agttcaaagc gagtgataac cggccccgga
    11221 gagtaattga cgacatcggc tttaatacgg aaatcagcca gacgcgcttc caccaggcga
    11281 gccatttgtt caagcgcaaa ggtatctacc ggctccactt cgctcggcgg cggtgtcagc
    11341 aaatccagag aaggcagcgg cgtcgtcggt ttatgcaacg gacggctgtc gccattacgc
    11401 atcaacagcg gatgaagcag ggtatcctgc tgctgcggtg caaccggttg ttgcggctgt
    11461 tgatactgcg gctgcggagc aactggttgt tgcggctgct gatattgcgg ctgtggcgca
    11521 accggttgtt gcggctgctg atattgtggc tgcggcgcaa cctgctgttg tggctgctga
    11581 tactgcggct gcggcgcaac cggctgttgc ggctgctgat attgctgctg tggcggaact
    11641 ggttgttgcg gctgctgata ctgctgctgc ggtgcaaccg gttgttgcgg ctgctgtaca
    11701 ggttcaacaa ttggcgtaaa caacggttcg tgtggaccat catccagcaa cgctttcatt
    11761 ggcgaaaatt caaaatcatc cagcgagaac ggattcgccc cagccggttg ttcgccggaa
    11821 taacgttgtt gttgagtttg cgcaaactga cgagccagtt cagcctctgc cgcagcatct
    11881 gcatcttctg cgtttacggg cacatcatgt tgatactgtt cgccatagcg ttgctgctgt
    11941 gtctgggcga actgacgtgc cagttcatcc tgctgcatcg catcgatttc atcatcgtta
    12001 tactgatcgc cagaatcata ctgattgcgc tgggcttcac gggctttctc ttccgccgca
    12061 cgctgtgagg gcagcttaat accgtaagac gccagttcac gacgagttgg cacgcggata
    12121 cgtttcggtc gtggcaactg cgggccaatc ccctctttga cttgcggacg cggtccaccg
    12181 ctatttgcca gactgaagac tggcgcggca acggttgcgg cagcccccgt cgccagtgtc
    12241 gcttttttca cgccagatgc cagcggagaa acagcggcag cggcttctac tggaggtact
    12301 gctgcaacag aaggcgcttt caacgaagat ttgatcggtt ctggttcttt aaccggttct
    12361 ggaatcggtt gataccaggc cgcaagttgt tcacgttcac gggctcgctt ctcttccact
    12421 tcttcaaaat agtaaagcgg cggacgcgcg ggttttgtct cttctacaac gggttcaggc
    12481 tccacaacag gctgctgttc aacggattgc ggctgttggt acaacggctc ctgagcggct
    12541 ggttgctgat aagtttgctc agtctggtat gtagactgtg gagcaaaagt ggattgctgc
    12601 tcttcggctt gccaggcgtt acctgccacc ggttgttctg gcgcaggggc gtaatacggc
    12661 tgttgtgcag gctgttccgg cgcagttgcg taatacggtt gctgaacagg ttgttccgcc
    12721 gcaggagcgt agtaaggttg ttgcgcaggt tgttcagctg caggtgcata atacgactgc
    12781 tgcggctgta ctggttgttg cagcggctca ttatattgca ctgcaggctg cgcatattgt
    12841 gactgctgtg ggtaaccttc cggtgcagga gcaataaccg gctctcccgt ttgtggaccc
    12901 ggtacaggct gccaggctac tgtaggttgc gagggtggaa catcaacaga ggcaacaggc
    12961 ggcgtctgag tcacaggttc aaccggcgca gcccagcttt gtgtcgccgt ggtagcagca
    13021 gctgctacag cgacaggttc ggtaattggc gcaccgttta ataatggatc gtattcgtca
    13081 tattctggct gcgttgcacg attgcccgaa aataggacgt cgtccgggtc ggcagccaca
    13141 ccgcgtgcag tgtaggtaat ctcttcgtca tcatccatcc gcttaccgga gaacaacgca
    13201 gcgtctgttt gccgccccat cggattaatg aatttttccg ccaaccgttt acgacgcgct
    13261 agcgcgccgc gaagaatacg ggcacggcgt gattcatgct gtttgccgtg gttttcatct
    13321 tcatactctt cgtcatcttc atattcatct tcatcgaccc aggtatcatc gcgacgggta
    13381 cgattactgg cgaaggtgag aatgtttaaa atccagccgc cgagtttttc agcaatggtc
    13441 acccatgacc aaccggtgaa cagcgtcagg cccgcagccc aaacgcagag cagcgcaata
    13501 gttcccccgc tactgtgtag cagtggttgt agcgtagtac ttagtaagct gccaatgacg
    13561 ccaccggagg caaaatacca gatatcgtca gcgttgattg ccgccagacc acaggaggta
    13621 aggatgagcg ccaaaacgcc aatgatgcgt agcgaaacgg caaaataatc aatgtattcg
    13681 tcgctggact gatgacgcca ggcaaaccaa caaccgccga caataatgac gggaatggtg
    13741 taagccatca cgccaaaaat aaagaacagc gtatctgcca accacgcacc gggcatccca
    13801 cctaaattat ggataggttc atgccaggcc gtttgcgacc agctggggtc cgaagggtta
    13861 aagcttagta aggcagccat caaccagacg gcaaacagga caataaggat cagcaacgcc
    13921 tccagaaggc ggcggccgct acttaacttt gtcaatgtga cttctttgtc ttcagtgtat
    13981 tcctggctca agaaaggctc tccaggttac aggcttttcc tgcccgatgc taaaacggac
    14041 aacagtacca ggttacccca gtactgttgc tgtatggatt aacaggagtg taatcaaaat
    14101 acgccgattt tgcacctgtt ccgtgttagc gcgtcttaat aaccagacga ttactctgct
    14161 tgacttcttc cataacaacg tatgtccgtg tgtcattgac gccaggcaga cgcagcaggg
    14221 tttcccccag caacttacgg taagctgaca tatccggcac gcgtgttttc aacaggtagt
    14281 cgaaatcacc agatactaaa tgacactcct gaatttcttc aagtttttgt acagcggtat
    14341 tgaattgttc aaacacatcc ggtgcgccac gattcagagt aatctcaacg aataccagaa
    14401 gtgatgcatc cagataatgg gggttcagca gcgccgtata gccctgaata aacccttgtc
    14461 tttccagccg acgcacacgc tcaaggcacg gcgttgggga aagtcccaca cgtttagaaa
    14521 gctcgacgtt agaaatacgc ccatcctttt gcaactcatt aagaatgtta cgatcgatac
    14581 ggtcgagatc tttgccaggg cgcttcttgc tatctaccat tattattgtc tctctgtatt
    14641 ccttccctac tcctgtctga ccctgaaaca tcactgttca gagtcgctac ccgtcacgat
    14701 agaccgaaat catgaagatt cacggcgcgc aatgcctggg tctacggtga gaagaccgtt
    14761 gccttgtggc agctatcgac atcacgaatg gcgtctgtcc acgccatacg tagatttcac
    14821 tagcacggta aacatggtat ttacatgcat ggttatgcgc atataagaat actgtttttt
    14881 tcttcctcga atgttttcgc aaaacaccag gggattgtca aagcaaaaca tcgattttta
    14941 gtacaacatg ccagatattc attaacaggg gcgtttattc atcatttaat cggcttataa
    15001 agcgggaaat ttaagtagca gaggtgataa tgacgggaga atttgacctg cttatcgatg
    15061 ctgccgattg cacaaattgt taacaaaatc gtcatactct ttttttacgt ctgtaaattc
    15121 cctacaatcc tgcccattgt ctgccaacaa ctatggggat ctcatgggca cgaccaaaca
    15181 cagtaaactg cttatcctgg gttcaggccc ggcgggatac accgctgctg tctacgcggc
    15241 gcgcgccaac ctgcaacctg tgctgattac cggcatggaa aaaggcggcc aactgaccac
    15301 caccacggaa gtggaaaact ggcctggcga tccaaacgat ctgaccggtc cgttattaat
    15361 ggagcgcatg cacgaacatg ccaccaagtt tgaaactgag atcatttttg atcatatcaa
    15421 caaggtggat ctgcaaaacc gtccgttccg tctgaatggc gataacggcg aatacacttg
    15481 cgacgcgctg attattgcca ccggagcttc tgcacgctat ctcggcctgc cctctgaaga
    15541 agcctttaaa ggcagtgggg tttctgcttg tgcaacctgc gacggtttct tctatcgcaa
    15601 ccagaaagtt gcggtcatcg gcggcggcaa taccgcggtt gaagaggcgc tgtatctgtc
    15661 taacatcgct tcggaagtgc atcttattca ccgccgtgac ggtttccgcg cggaaaaaat
    15721 ccttattaag cgtctgatgg ataaagtgga gaacggcaac atcattctgc acaccaaccg
    15781 tacgctggaa gaagtgaccg gcgatcaaat gggtgtcact ggcgttcgtc tgcgcgatac
    15841 gcaaaacagc gataacatcg agtcactcga cgttgctggt ctgtttgttg ctatcggtca
    15901 cagcccgaat actgccattt tcgaagggca gctggaactg gaaaacggct acatcaaagt
    15961 gcagtcgggt attcatggta atgccaccca gaccagcatt cctggcgtct ttgccgcggg
    16021 tgacgtgatg gatcatattt atcgtcaggc cattacttcg gccggtacag gctgcatggc
    16081 agcacttgat gcggaacgct acctcgatgg tttagctgac gcaaaataat tttacaaatc
    16141 agtaacaaaa gtaaagaagg cgacaccatg cgactatggg tcgcctttat tttttccccg
    16201 ttgtaacatt gctctgcaaa taattctgat aactcacctg ctaagcgtgc aatgaataaa
    16261 tcccgtcaaa aagagttaac ccgctggtta aaacagcaaa gcgtcatctc ccaacgttgg
    16321 ctgaatattt ctcgtctgct gggctttgta agcggcatat tgatcattgc ccaggcctgg
    16381 ttcatggcgc gaattctgca acatatgatt atggagaata ttccccgtga agccctgctg
    16441 cttcccttta cgttactggt tctgaccttt gtactgcgcg catgggtggt ctggttacgc
    16501 gaacgggtgg gttatcacgc cgggcagcat atccgctttg ccatccgccg tcaggttctc
    16561 gaccgtctgc aacaagcagg gccagcgtgg attcagggta aacctgcggg gagctgggcg
    16621 acgttggtac tcgagcaaat tgacgatatg catgattact atgcacgcta cctgccgcaa
    16681 atggcgctgg cagtgtcggt gccgttgctg attgtggtgg cgatcttccc ctctaactgg
    16741 gctgcggcgc tcattctgct gggcacagca ccgttaattc cgttgtttat ggcgctggtt
    16801 gggatggggg ctgccgatgc taaccggcgt aactttctcg ctcttgctcg cttaagtggg
    16861 catttcctcg atcgcctgcg cggcatggaa acattgcgta tttttggtcg tggtgaagct
    16921 gaaattgaaa gtattcgttc tgcttcggaa gatttccgcc aacggacaat ggaagtgcta
    16981 cggctggcgt ttttatcctc cggcattctc gaatttttta cctcgctgtc aattgctctg
    17041 gtggcggtct actttggttt ttcctatctc ggcgagctgg attttggtca ctacgatacc
    17101 ggtgtgacgc tggctgcggg ttttctggcc ctgatccttg cgccagagtt tttccagcca
    17161 ttgcgcgatc tcggtacgtt ttatcatgct aaagcccagg ctgttggtgc agctgacagt
    17221 ctgaaaacgt ttatggaaac cccgctcgcc catccgcagc gtggcgaggt ggaattagca
    17281 tcgaccgatc cggtgaccat tgaggccgag gatctgttta tcacgtcgcc ggaaggtaaa
    17341 acgctggccg gaccgctgaa ctttactttg ccagcaggcc aacgtgcagt gttggttggt
    17401 cgcagcggtt caggtaaaag ctcactgctg aacgcgcttt ctggttttct ctcatatcag
    17461 ggatcattac gaatcaacgg gatagaatta cgcgatttat caccagaatc atggcgtaaa
    17521 catctctcct gggttgggca aaacccacaa ttaccggcag caacattgcg ggataacgta
    17581 ctactggcgc gacctgatgc cagcgaacaa gaattacaag cagcgctgga taacgcctgg
    17641 gtcagcgagt ttctaccgct cctgccacaa ggcgttgata cacctgttgg cgaccaggct
    17701 gcccgccttt ccgtggggca ggcgcagcgc gtggcggtgg cccgtgcgtt actaaatccc
    17761 tgttcgctat tactgttgga tgaacccgct gccagccttg atgctcacag tgaacagcgc
    17821 gtaatggagg cgctgaatgc cgcctctctg cgccagacaa cgttaatggt cacccaccag
    17881 ttagaagatc ttgctgactg ggatgtcatt tgggttatgc aggatggccg gattattgag
    17941 caaggacgtt acgcggaatt aagtgtggct ggtggcccat tcgccacatt actggcccat
    18001 cgtcaggagg agatttaaat gcgcgctttg ctaccctatc tggcactgta taaacgtcat
    18061 aaatggatgt taagtcttgg tatcgtgctg gcaattgtca cgctgctcgc cagtatcggt
    18121 ctgttgacac tttccggctg gttcctctcg gcctcagcgg ttgcgggggt tgccggactg
    18181 tacagcttca actatatgct acccgctgcg ggcgtgcgtg gcgcagcaat cacccgtact
    18241 gccgggcgtt attttgaacg tctggtaagt cacgacgcga ctttccgcgt gttgcagcat
    18301 ctgcgcattt acaccttcag caaattgctg cccctctccc ctgccggact ggcgcgctat
    18361 cgtcagggcg aattgctcaa tcgcgtggtg gcggatgttg atacgctcga tcatctttat
    18421 ctgcgcgtta tctcgccgct ggtgggcgct tttgtggtga ttatggtggt gacaatcggg
    18481 ttaagtttcc ttgatttcac cctcgccttt aggctgggcg gcattatgtt actgacgctt
    18541 ttcctgatgc caccgctgtt ttatcgtgcg ggaaaaagca ccgggcaaaa tctgactcat
    18601 cttcgcggac agtatcgcca acaactgacg gcctggctgc aagggcaagc tgagctgacc
    18661 atttttggcg ccagcgatcg ttatcgcacg caactagaga atacagaaat tcaatggctg
    18721 gaagcgcaac gccgtcaatc tgaactgacc gcattgtcgc aagcgataat gctgctcatt
    18781 ggcgcgttag cggtgatcct gatgctgtgg atggcgtctg gcggcgttgg cggcaatgct
    18841 caacccggcg cgttaattgc cctgtttgtc ttctgcgcgt tagccgcgtt tgaagcactg
    18901 gcaccagtaa cgggtgcatt tcagcatctg gggcaagtca ttgcctctgc cgttcggatc
    18961 actgatttaa cggatcaaaa accggaagtc accttccctg atacccaaac tcgtgttgcc
    19021 gatcgcgttt cgctgacatt acgggatgtt cagttcactt atccggagca atcccaacag
    19081 gcacttaaag ggatttctct tcaggtaaac gccggggaac atattgcgat tctcgggcga
    19141 accggatgcg gcaaatcaac actgttacaa ctgctgaccc gcgcttggga cccgcaacag
    19201 ggagagattt tgcttaacga tagccccata gccagcctga atgaagcggc tctacgacag
    19261 accatcagcg ttgttcctca gcgagtgcat ctgtttagcg ccacgctgcg tgataatctg
    19321 ttactcgcct cgcctggcag tagtgatgag gctctggcgg agagcttgcg tcgcgttggt
    19381 ctggagaagc tgctcgagga tgcaggtctc aacagttggt taggtgaagg cggacgccag
    19441 ctctccggtg gtgaactgcg ccgtctggct atcgcccgtg cgctgttaca tgatgcgcca
    19501 ctggtgttgc tggatgaacc taccgaaggc ttagatgcca caaccgaaag ccagatcctt
    19561 gaattgcttg cagaaatgat gcgtgagaaa acggtgttaa tggtcaccca tcgacttcgc
    19621 ggactctctc gtttccaaca aataatagtg atggacaacg ggcaaattat tgagcaaggt
    19681 actcacgcag agctgcttgc cagacagggg cgttattacc agttcaagca gggtttgtaa
    19741 gctattattg aacgatccga cttgcgtgga gttttgcggt catgcgcctg gttcagcttt
    19801 ctcgccattc aatagccttc ccttccccgg aaggcgcatt acgtgagcct aacggcctgc
    19861 tggcacttgg gggcgatctt agccctgcgc gcctgttaat ggcttaccag cgtggtattt
    19921 ttccgtggtt ttctccaggc gaccccatcc tctggtggtc gcccgatccc cgcgcggtgc
    19981 tatggccaga atcactgcat atcagccgta gtatgaagcg atttcataaa cgctcgccct
    20041 atcgtgtcac gatgaattac gcttttggtc aggtcattga aggctgtgcc agcgatcgcg
    20101 aagaaggaac ctggatcacg cgtggcgtgg tcgaagccta ccatcgcctt cacgaactcg
    20161 gccatgccca ctccattgaa gtctggcgtg aagatgagct tgtcggcggt atgtacggcg
    20221 tggcccaggg aacgctattt tgtggcgagt ccatgttcag ccggatggaa aatgcgtcta
    20281 aaacggcgct tctggtattc tgtgaggaat ttatcggtca tggcggtaag cttatcgact
    20341 gccaggtcct taacgatcac acagcatcgc ttggtgcctg cgaaattccc cgccgggatt
    20401 accttaatta tctcaatcaa atgcgcctcg gacgattgcc gaataatttc tgggtaccac
    20461 gatgcttgtt ttcaccacaa gaatgaatgt tttcggcaca tttctcccca gagtgttata
    20521 attgcggtcg cagagttggt tacgctcatt accccgctgc cgataaggat tttttcgcgt
    20581 caggtaacgc ccatcgttta tctcaccgct cccttatacg ttgcgctttt ggtgcggctt
    20641 agccgtgtgt tttcggagta atgtgccgaa cctgtttgtt gcgatttagc gcgcaaatct
    20701 ttacttattt acagaacttc ggcattatct tgccggttca aattacggta gtgatacccc
    20761 agaggattag atggccaaag aagacaatat tgaaatgcaa ggtaccgttc ttgaaacgtt
    20821 gcctaatacc atgttccgcg tagagttaga aaacggtcac gtggttactg cacacatctc
    20881 cggtaaaatg cgcaaaaact acatccgcat cctgacgggc gacaaagtga ctgttgaact
    20941 gaccccgtac gacctgagca aaggccgcat tgtcttccgt agtcgctgat tgttttaccg
    21001 cctgatgggc gaagagaaag aacgagtaaa aggtcggttt aaccggcctt tttattttgt
    21061 gatatgtatg aagtactttg gaagtataag tccgtaactt gtctcgatgt agatatactg
    21121 cacaacttgt ttacagtatg agcaaacaat aattatcatt aaaattcttc ttgaccgttt
    21181 tactccaact ccctatagta gcgccccgtt gccacccatg aggtttggta gcaaaacaat
    21241 ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta
    21301 tcgggggttc gaatcccccc ctcaccgcca tatttaaaga agagctcgta cgaaagtgcg
    21361 agcttttttt tcgcatttta tctgtctgta cggcagtgaa cgcgttcgca aaggttttac
    21421 cgcaccccgt cgatttctaa gctgcctgta cggcagtgaa ctcaaatact acacgtttag
    21481 cccgttgtta ataaagcaaa accatcccat tctcctgcag cacccttttt tatccttacc
    21541 ttcccgcagc actaattatc aacaacttac catcatcatt aaaaaagggt ctgaacctcc
    21601 tccaccccat aaacaaaaag tccgtaacct ctttcgagat tacggacttg accaacctac
    21661 ctaacaatca gattaatgcg ctgcttccgc cttgtgcttt tgtgcactct ggaatccgta
    21721 agtcagctca tttttctctt tatccagcgc gacggtgacc tgaccgccgt ccaccagcga
    21781 accaaacagc agttcgttgg cgagcggttt tttcaggttg tcctggatga cacgcgccat
    21841 cggacgtgcg cccattgccc ggtcgtaacc tttctcggcc agccagttac gcgcttcctg
    21901 gctcacttcc agagaaacac ctttctgatc cagctgaacc tgcaactcga cgatgaattt
    21961 atccaccacc tgatggatca cgtcggttga cagatgatca aaccagataa tgttgtcgag
    22021 acggttacgg aattccggtg taaagatctt cttgatctcc tccatcgcat cggtgctgtt
    22081 atcctggtgg ataagaccaa tggatttacg ctcagtttca cgtaccccgg cgttggtggt
    22141 catcaccagc accacgttac ggaagtccgc tttgcgtccg ttgttatcgg tcagcgtacc
    22201 gttgtccatc acctgcaaca gaatattgaa cacgtccgga tgcgctttct cgatttcgtc
    22261 cagcaacagc accgcatgtg gatgcttgat gaccgcatca gtcagcaaac ctccctgatc
    22321 aaaaccaacg tatcccggag gcgcaccaat cagacggctg acggtatggc gttccatata
    22381 ctcggacata tcaaagcgca gcagctcaat gcccaacgct ttcgaaagct gtaccgtcac
    22441 ctctgttttc ccgaccccgg tagggccggc aaacaggaac gaaccaaccg gtttatgttc
    22501 gtgacctaaa cctgcacgcg ccatcttaat ggcttcagtc agcgcctcaa tggctttatc
    22561 ctgaccgaag accagcattt tcaggcgatc gccgaggttt ttcagggtat cgcggtcact
    22621 ctgagaaaca ctcttctctg gaatgcgcgc aatacgggcc accacggact caatatccgc
    22681 cacattaacg gttttcttgc gtttgcttac cggcatcagg cgtgcgcgag cgcccgcttc
    22741 gtcgataacg tcaatggctt tatccggcag atgacgatcg ttaatgtatt tcaccgccag
    22801 ctctaccgct gcacgcaccg cttttgcggt ataacgcacg tcgtggtgcg cttcatactt
    22861 cggtttcagg ccattgatga tttgaacagt ttcttcgatc gacggttcag taatatcaat
    22921 tttctggaag cgacgcgcca gagcacggtc tttctcgaaa atgttgctga actcctgata
    22981 ggttgtcgaa ccaattacgc gaattttacc gctggagagc aacggtttga tcaggttagc
    23041 cgcatcgacc tggccaccag acgctgcacc cgcaccgata atggtgtgga tctcatcaat
    23101 aaacaggatg ctgttagtgt cctgctccag ctgcttgagc aacgctttaa aacgtttttc
    23161 aaagtcgccg cgatatttag tgcccgctaa cagagaaccg atatcgagag agtaaatcgt
    23221 acagtcagcc atcacttccg gcacatcgcc ctgaacaatt cgccaggcaa gaccttccgc
    23281 aatcgcggtt ttaccgacac cagattcccc caccagcagc gggttgtttt tacggcgacg
    23341 gcagagaacc tgaatagcac gctccagctc cttctcacga ccaatcagtg ggtcgattcc
    23401 gcccacgcgc gcaagctgat tcaggttcgt cgtgaaattc tccatacgtt cctccccacc
    23461 agcttgttct tcgctgtttg gctggctgcc aggatcagaa gactgtgtcg gctcgtcttt
    23521 acgcgtgcca tgagagataa agttcaccac atcgagacgg ctgacttcat gtttacgcaa
    23581 cagatatgcc gcctgcgact cctgttcgct aaagatagcg accagaacgt ttgcaccggt
    23641 tacctcattg cgaccggagg actggacatg gaagaccgca cgttgcagta cacgctgaaa
    23701 actcagcgtc ggctgtgtgt cgcgctcctc ttcactggca ggcagaacgg gtgtggtttg
    23761 ttcaataaag gcttccagtt cctgacggag cgcaaccaaa tccacagaac acgcttccag
    23821 cgcctcccgg gcagatgggt tactgagcag cgccagtaac aagtgctcga cggtcataaa
    23881 ctcatgacgg tgctcgcgcg ctctggcgaa agccatattt aaactgagtt ccagttcttg
    23941 attgagcata ggcacctccc ccaattttta tgcctgcatt caggcttttt ctagcgtaca
    24001 cagcaatgga tgctcattct ccctcgcgta cttgttcacc atcgccactt tggtttctgc
    24061 aacctcggcg gtaaagactc cgcaaatggc cttcccctgg tagtgaacag cgagcatcaa
    24121 ttgcgttgca cgttctacat cataagaaaa gaatttttgt aacacgtcaa taacaaactc
    24181 catcggagtg taatcatcat tgactaatat cactttatac atagatggcg gttttagcgc
    24241 gtcgcgaact ttttcttccg ccagttgatc aaagtccagc cagtcgttcg ttttacccat
    24301 tgtcagttat catcttcggt tacggttatc ggcagaacgc cctgccgctg acaagagctt
    24361 atttgcctga ccaatctact ccacatgata gataactatc atctatcgtt ggcatcagcg
    24421 acatctgtca cattcctgtc aatagcgtta actgcttcaa atttttgatt catacctgcc
    24481 ccctggcccc tgtcaaacgc ttgacggctc gccctaattc tctaaattgt atttctagag
    24541 ttggcgaggt tttgaacagc cccctctctg accccggttt attccatctt acttgtataa
    24601 gatttgcgaa ggatgtcgaa gcatggaaaa gggtactgtt aagtggttca acaatgccaa
    24661 agggtttggt ttcatctgcc ctgaaggcgg cggcgaagat attttcgctc attattccac
    24721 cattcagatg gatggttaca gaacgctaaa agctggacaa tccgttcagt ttgatgtcca
    24781 ccaggggcca aaaggcaatc acgccagtgt tattgtgccc gtcgaagtag aagcggcagt
    24841 cgcatagctc ttctgtctca ttgtgtacat cctaaaggca aaatgccagc ccgatcggct
    24901 ggcattttta tctcaaaaat tactctcgtg ccagagcatc tactggatcc agtcgtgccg
    24961 catttcgtgc gggtaaccat ccaaataaaa tcccggtgac cgtcgagcag agaaacgcca
    25021 gcagcagtgc caacggtgaa aaaccaatct cccagccggg taagaaaagc tgcaaggtga
    25081 aagcaattaa cagtgacagt gttattccca acgcgccacc gaccaggcaa accagtacgg
    25141 cttcgatcag gaactgttgc aaaacatcgc ttgctcgcgc acctacagcc atgcgaatgc
    25201 caatttcccg cgtccgctcg gtcactgaca ccagcataat attcattaca ccaataccgc
    25261 ccaccaccag cgaaatcacc gccaccagcg tcagaaacag ttgtaaagta cgtgtggtct
    25321 tttcaacagt tttcaagacg ccgtccatgt tccaggtaaa gaaatccttc tttccgtggc
    25381 gcagtgaaag taaacgcgtg agttgctgtt ccgcctcggc gctgtcaaat ccttctttta
    25441 ccctgacagt aatggagttc agccacgact ggcccataac tcgcccggac atcgtgctgt
    25501 aaggtagcca gacacgcagc actttactgc taccaaacat cgactgtttt tcttccgcca
    25561 caccaatgac tctggcgggc atattgccga ccagaatcac ctcgccaacc acatctgctt
    25621 tatgggggaa aagctggcgg cgagtattac tgtcgagaac cacgacctgc gcacgaccgt
    25681 tcagctgctc ctgattaaag gtgtttcctt cactgaaggt catgccatag acattaaaat
    25741 aatcgccgct cacgccattg gcactggcag caacatcaac attgttataa cgcaggcgca
    25801 ggttttgcga gacggcaggt gtggctgagg cgacccacgg ttgtttttgg atggcgatta
    25861 agtcgtcgta cttcagcgcc tgctgatatt gcggatcgtc atcgccaaaa tctttcccgg
    25921 gatagacatc aatagtattc gtaccaatag aacgaatatc cgccagcacc atttgtttgg
    25981 cggcgtcacc caccacgaca atggaaacca ccgacgcaat accgataata atccccagca
    26041 tggtcagtaa agtacgcatt ttattcgctg ccagcgcccg ccatgccatc gtcagcgcct
    26101 cgttaaaacc gctgacaaac tgccgccagc cagacaccgt gttgacaaca ggctccgtcc
    26161 cgccagcaac attcactttt tcaatggcgg gaggattgcg cacaatttcg ccgtcgcgaa
    26221 tttcgatcac ccgctcggcc tgagcagcga cctgcggatc gtgggtgacg ataatcaccg
    26281 tatgcccacg atcgcgcagc tgatgcagga tcgccatcac ctcttcgcca gaatggctgt
    26341 ccagtgcgcc ggttggttca tcggcaagaa ttacctgacc accgttcatc aatgcccgcg
    26401 cgatgctgac gcgctgttgc tgaccacccg aaagctgtgc cggataatac tctgtacggt
    26461 cttccagccc cagccgttgc agcaactcct gggcacgaag cagtcgctgt ttccgctcaa
    26521 gaccagcata gacggcgggt acttcaacgt tctgctcggc ggttaaatgc gaaagtaaat
    26581 ggtaacgctg gaaaataaag ccgaaatgct cgcggcgcag ttgcgccagc gcatcggcgt
    26641 ccagtgtggc aacatcctga ccggcgacgc gataggtgcc gctggtggcc ttatccagac
    26701 agccgagaat attcatcagg gtcgatttac cggaacctga agcgccaaca atcgctacca
    26761 tctcaccggc ataaatatcg agggtgatgc ccttcagcac ctcaacctgc tcatcaccgg
    26821 caggatagct gcgacgaata tcctttaatt cgagcaaagg cgtcattgtg cagctcctgg
    26881 tttggcctca ccaatcacca cttcatcgcc cgcttcaagc cctttgacaa tctcaacatc
    26941 ggtatcgtta cgtgcgccaa tcgtcacttc acgctcgcgt gtttcaccat tacgcaacaa
    27001 tttgacttta taacgattat cgccaaccgg atcgcctaac gccgacagag ggatcgtcag
    27061 cacatttttc acatcggtga gctgaatatg cacttgcgca gtcatatcca gccgcagcaa
    27121 accattgggg ttggggactt caaaacgggc gtaatagaaa atagcgtcgt taaccttttc
    27181 cggcgtcggt agtacatcct tgatttgccc ctcgtagcgc gtcagtggat cgccaagtac
    27241 cgtaaaccag gctttttgcc ccggcttcag gtggattaca tccgcttcag aaacctgcgc
    27301 ttttaccagc atggtgctca tatctgccag cgtcagaatg ttcggtgctt gttgtgcggc
    27361 aatcaccgtc tggccttgca gagtggtgat ttgcgtgact tccccggcca tcggggcaac
    27421 gatgcgagtg taatcgagat tggttttagc cgtatcgaga gaggcctgat tgcgcttgat
    27481 ttgcgcgtca atggtgccaa tttgcgcctg tttcacagcc atctccgtcg cggcggtgtc
    27541 gagatcctgc tgtgaaaccg cctgcgtttg tgccagacgt tgctgacggg aatacgtcac
    27601 ccgcgccagt ttcagctccg cttccgcctg ctgccgctgc gcacgtagct ccatcagcgt
    27661 tgcttcgacc tccttaatct ggttttcagc ctgttcagga tcaataaccc ctaaaagctg
    27721 gtcttttttt actttatcgc caatcgccac cgacagagtt ttcaactgac cgctgacctg
    27781 cgcgcccacg tcaaccttac gcagcgcgtc cagctttccg gtcgccagca cgctttgctg
    27841 taaatcaccg gggcgcacaa tcagtgtctg ataagtcggc acgggtgcgt taagaattct
    27901 ccataacgta atcagtccgg cgatgactat caccagcgca ataacgtaac gcttcttcac
    27961 ggttttccgc tttttcataa atattctgaa tactccatat gccaatccgg gtcattaact
    28021 tcaacgaaat atcaacaaaa ccctaaaatt tacgttagta atcacactat tgatgtttgg
    28081 ttaagatacg caatggtgaa atcgacgtca tgtataacca ttgatttcat gaatatgtcg
    28141 cagctaactg aacggacctt tacgccatct gaatctctca gcagcctgtc actttttctt
    28201 agtctggcac gtggacagtg tcggccgggt aaattctggc atcgccgtag ttttcgccag
    28261 aaatttttgc tgcgctcgtt gattatgccg cgtttaagcg ttgagtggat gaacgaactt
    28321 tcccactggc ctaatctcaa tgtgttgtta acgcgccaac cgcgactgcc tgtgcgtctg
    28381 catcgccctt accttgcggc gaatcttagc cgtaagcaat tgctggaggc gttacgttac
    28441 cattatgcgt tactccgcgg atgtatgtcg gcggaagaat tcagcttata tttgaatacc
    28501 cccgggctgc aactggctaa actggaaggc aaaaacggcg agcagttcac gcttgagctg
    28561 accatgatga tctcaatgga taaagaaggt gacagcacaa tcctgttccg caacagcgaa
    28621 ggtattcctc tggcagagct cacttttacc ctgtgtgaat atcaggggaa aagaacgatg
    28681 tttattggcg gactgcaagg cgcaaaatgg gaaatcccac atcaggaaat ccagaatgcg
    28741 acgaaagcct gccacgggct atttcccaaa cgcctcgtga tggaagcggc ctgtctgttt
    28801 gcccaacgtt tgcaggtaga gcagattatt gccgtcagca atgaaacgca tatttaccgc
    28861 agcctgcgtt atcgtgataa agaaggcaag atccatgccg attacaacgc tttctgggaa
    28921 tcggttggcg gcgtatgtga tgctgaacgc cattaccgcc ttccagcaca gatagcacga
    28981 aaagagattg ccgaaatcgc cagtaaaaaa cgggctgaat accgtcggcg ctatgagatg
    29041 ctcgacgcta ttcagccaca aatggccacg atgtttcgcg gttaatccgc gcgaccgcgc
    29101 gccagccaca gcacgcgtga gaacattttt ttcagcagcg tcggtacgga gtccacacca
    29161 cgacgtccgg cctccattgc cacttcaatg gcaagatcgg gtttggaaga gctatggatc
    29221 gctttcgaga taattttgcg cagattcatc ggtacatttt ccgggatttg cgccacgcgg
    29281 tggaacacat cggaaaatcc ctggcgatac ataaaatgtt ccatatccag cgccggtaac
    29341 gccgttaaat gttctcgttc ggcttcccga tcgttattca acaggctgcg taccgtagcg
    29401 gcatatttct tccctgcttc atcgccatcg accagtacat gccattcaat ccccattcgg
    29461 cgggcaaatt taaccagtgg ctttagcccg gactgggcaa actcaatgac cttgatccct
    29521 tcggcatcga aatggtgtcc acactggcgc gccagttcat tgataaccca ggtttccgtt
    29581 tccccttcca ccagcaacca gcagcgagca aacagcgatg acggacggtt aaaacgaatg
    29641 tgaaaggata tgcgtcggct atcttcggta ctcaagccac tcggccccag acgccaggcg
    29701 gcaacgcgcg aggactcacg taccagtcgg caaacatgct ctaccggcgt taacgaaagc
    29761 aactcacccg agttggtggt ggcaatgcgc tgcaatggca gaagattcaa cagatgccag
    29821 gcaactgaaa gcataatggg gtgtaaacgg gtttctggat cttcaattag caataacgga
    29881 cgcgcgtctt tatccagtct caatgtgcct tttgcctgca ataaagtagc aaacaggccg
    29941 agcaaaataa cccgatacga gcgccctcca ggtcggtcaa tcatccggtt gatgatgtcc
    30001 agatagcgcc agcttcgttg ctcattgctg gcccgccgcc gcattaaacg atatcgcgcc
    30061 tgtccagccc cctgctcaga gaaataatgc tcaagcaact gcaccattgc ggaaagtccc
    30121 tgacgaatct gtccatcaga gagattttgc ggatgtgagg acaactcacg ggcgaggaaa
    30181 tcgagctggc gcgcggtgac ttccacattg ggaacatttg gcaccgtgcc gttacgaata
    30241 cggcgcataa aacgggcatc acgcaagcgc agcaccggca ttaaacgcac cagatggcgt
    30301 gcctgatcgt taatatcctc gacatcaatc ggatgtccgt ctttatcgag aaaactgcgc
    30361 agtgtcatca cgctgccgtc ttccgcactc tccccttcca gacgataaaa aatacggtga
    30421 tagccatcgg tgcatggcgt ccagcacgct tccagcggcc gataacggcg aacccgatgt
    30481 cggcctggca gcgattcgcg gaaggtcaaa ataatatgca gatgatgttc tcgcccgttg
    30541 atatctcccg gcgggaacca gaaatcgtcg cgctcaaaat ggtagagatc tgattctggc
    30601 gatagcagca gggttaaggc gtccagcaag ctggatttac cccacgcgtt ctccccaatc
    30661 aggacgttgt tttgttcgag catcaacgac aaacggttga taccgcgaaa acccacaatt
    30721 tcaacgcgct caagaatcat atcccctcca gaaatatgtg acttatcctt tacgctggca
    30781 gtataacggg aaaggccttg ctaatacgtg atgacatcac atttaagcat ggcaaatgtc
    30841 tgaaaaatga gcgaataatc gcgttatact ccctcttgct gataagtctg caaaaacaac
    30901 aaaatcaaat aattagcatg ataaattatt ttttattacg cctaaaggat taaatctttt
    30961 gcttgcacta cactgtcaat cagacaaatg actatatggg gcaacccgct gttgccctaa
    31021 atcaaattta ctggatttat ttatctggtg aatgactcgc cttcgttttt aaaatagccg
    31081 cgcattattt ccgtcgtcac ttttttacga cgaccctata aaacgaccat atttttcaca
    31141 gggtcaattt ttaattgagg tggatatgtt cagaaaatta gcagctgaat gttttggtac
    31201 tttctggctt gtttttggtg gctgtggtag tgctgtactg gccgcaggct tcccggaatt
    31261 aggcattggt tttgccggcg tggcgttggc gttcggtctg accgttctga cgatggcctt
    31321 tgctgttggt catatttctg gtggtcattt taacccggcg gtcactattg gtttatgggc
    31381 tggcggacgt tttccggcaa aagaagtcgt tggctacgta attgcccagg ttgtcggcgg
    31441 tattgttgca gcggcgctgc tgtatttaat tgccagtggt aaaacgggtt ttgacgcggc
    31501 agccagcggt tttgcttcta acggttatgg cgagcattca ccaggcggtt attccatgct
    31561 ttccgcgctg gtagttgaac tggtattgag tgcaggtttc ctgttggtga tccacggcgc
    31621 aaccgacaaa ttcgcgccgg caggttttgc gccgatcgct attggtctgg ccttaaccct
    31681 gattcactta attagtattc cggtgactaa cacttctgtt aacccggcgc gcagcaccgc
    31741 ggttgctatc ttccagggcg gctgggcatt agaacaactg tggttcttct gggtggtgcc
    31801 aattgtcggc ggcattatcg gtggtctgat ttaccggacc ctgctggaaa agcgtgatta
    31861 attaacgcag aaggcctggc gtaatgtcag gccttttttt aagatgttgt tatatgttga
    31921 ttcatcatct tgttgcgctc ctttcatgag ctaagccaca tattgccact ggcgcaagga
    31981 gcgcgcaggg ggcggccaat cgccgccgcc ccatgcaccc ccgggctctg gcgaacaaaa
    32041 tcgccgctgc gcggtgccct cgacttatcc cttacggcta ccgggtcggg cgcgaggtaa
    32101 catccctgta aaacgcgccc tcagcccaca tccatgtggg ctgccccggc cttcagggaa
    32161 cgcctcggcg attttgacgc caccaacatc ggtgcagttt gtaattaaag cgcaagaata
    32221 tctttaaatg aaaacgaata ctctactgct ccttatgcat aacatttcct tttaattgca
    32281 actttactcg tccttccgct ttcagtagtg tgtcatcgcg catttcgact tcatctttgc
    32341 aaggactggg ttttcatgtt ttctgggctg ttaatcattc tggttcccct gattgtgggt
    32401 tacctcattc cgcttcgcca acaagctgcg ttaaaagtta ttaatcagct attaagctgg
    32461 atggtttacc ttattctctt ttttatgggt ataagtctgg cgttcctcga taacctcgcc
    32521 agtaacctgt tggcgattct gcattattct gccgtcagta ttaccgttat tttactgtgt
    32581 aatattgccg ccctgatgtg gctggagcga ggcctgccgt ggcgcaacca ccatcagcaa
    32641 gaaaaactcc cgtcgcgtat tgcgatggcg ctggagtcgc taaaactgtg cggcgtagta
    32701 gtgattggtt ttgccattgg tctgagtgga ctggctttct tacaacacgc gaccgaagcc
    32761 agtgaataca cgttaatttt gctacttttc ctcgttggta ttcagttgcg caataatggc
    32821 atgaccttaa agcagattgt ccttaatcgc cggggaatga ttgtcgccgt ggtggtggtt
    32881 gtcagttcat taattggtgg tttaattaac gcctttattc ttgatctccc catcaatacc
    32941 tcgctggcaa tggcctccgg tttcggctgg tattctcttt ccggtatttt attgaccgaa
    33001 tcttttggtc cggtaatcgg gagcgcggcg ttttttaatg atctggcccg tgaactgatt
    33061 gctattatgt tgatccctgg gctgattcgc cgcagccgct ctactgcact gggcttatgc
    33121 ggtgccacat caatggattt caccctgccc gttcttcaac gtactggcgg gctggatatg
    33181 gtcccggcgg caattgttca cggttttatt cttagcctgt tagtgccgat cctcatcgcc
    33241 tttttctctg cgtaatacct ctctggcggt agattcctgc cgccaaaatt gcgctaaatc
    33301 aatctccctt aaagttgcat gaaaaatccc ttttatcccc gcgttaagcg tcttaacctt
    33361 aaacatgtat attaaatata actttaaaag gtgtgatcat gttttgtgtg caatgtgaac
    33421 aaactatccg tactccggca ggaaacggct gctcatacgc gcaggggatg tgtggtaaaa
    33481 cggcggaaac ttctgacctt caggatttac tcatcgcggc gctgcaaggg ctttctgcct
    33541 gggcggtaaa agcgcgtgaa tacggcatca tcaaccacga tgttgacagc ttcgcgccac
    33601 gtgccttttt ctcaactctg accaacgtta acttcgattc tccgcgtatt gtcggctacg
    33661 ctcgtgaagc gattgccctg cgcgaggcgc tgaaagcgca atgcctggct gtagatgcca
    33721 acgcgcgcgt cgataacccg atggctgacc tgcaactggt gagcgacgat ctcggcgagc
    33781 tgcaacgtca ggctgcagaa tttaccccta acaaagataa agcggcgatt ggcgaaaaca
    33841 ttctcggcct gcgtctgctg tgcctgtatg gcctgaaagg tgcggcggcc tatatggaac
    33901 acgcgcatgt tctcggtcaa tacgacaacg atatttatgc ccagtatcat aaaatcatgg
    33961 cgtggctggg gacctggcct gccgatatga acgcgcttct tgagtgttca atggaaatcg
    34021 gccagatgaa cttcaaagtg atgagcattc tggatgcagg cgaaaccggt aaatacggtc
    34081 acccgacgcc aacccaggtc aacgtcaaag cgacggcggg taaatgcatt ctgatttccg
    34141 gtcacgatct caaagatctc tacaacctgc tggaacagac cgaaggcacg ggcgttaatg
    34201 tctacaccca cggcgaaatg ctgcctgcgc atggctaccc ggagctgcgt aaattcaagc
    34261 atctggtcgg taactacggc agtggctggc agaatcagca agtggagttc gctcgtttcc
    34321 ctggccccat cgtgatgacc tcgaactgca tcatcgaccc aaccgtaggc gcttatgacg
    34381 atcgtatctg gacccgcagc attgttggct ggcctggcgt gcgtcatctg gacggtgatg
    34441 atttctctgc ggttatcacc caggcgcaac agatggcagg cttcccgtac agcgaaattc
    34501 cgcaccttat caccgtgggt tttggtcgcc agacgctgct tggcgctgct gatacgctga
    34561 ttgatctggt gagccgtgaa aaactgcgtc atatcttcct gcttggtggc tgtgacggcg
    34621 cacgcggcga gcgccactac ttcaccgatt tcgccaccag cgtgccggat gactgcctga
    34681 tcctgaccct cgcctgtggt aaatatcgct ttaacaaact tgagtttggc gatatcgaag
    34741 gtctgccgcg tctggtagat gcaggtcaat gtaacgatgc ttactcagcg attattctgg
    34801 ctgtcactct ggcagagaaa ctgggctgcg gtgtcaacga tctgccgctg tcgctggtgc
    34861 tctcctggtt tgaacagaaa gcgatcgtca ttctgctgac gctgctttct ctgggcgtga
    34921 aaaatatcgt caccggcccg actgcacctg gtttcctgac accagacctg ctggcggtgc
    34981 tgaacgagaa atttggcctg cgttctatca ccactgttga agaagacatg aagcaactgt
    35041 tgagcgcgta aggagtttgt cgatgacgat gccaacgaat caatgcccgt ggcggatgca
    35101 ggttcatcac attacgcaag aaacgccgga tgtgtggacg atttccctga tttgccacga
    35161 ttactaccca tatcgcgccg ggcaatatgc actggtcagc gtgcgaaact cagcggaaac
    35221 gctgcgtgct tacaccattt cctccacgcc aggcgtgagt gaatatatca ccctgaccgt
    35281 gcggcggatt gatgacggtg tcggctccca gtggctgacg cgcgatgtaa aacgcggtga
    35341 ttatctctgg ctttcggacg cgatggggga atttacctgc gacgataaag cagaagataa
    35401 attcctgttg ctggcggcag gctgcggcgt cacgccgatt atgtcgatgc gtcgctggct
    35461 tgcgaagaac cgtccacagg ccgatgtgcg ggtgatctac aacgtgcgta cgccgcagga
    35521 tgttattttc gccgatgagt ggcgtaacta tccggtaacg ctggtggcag aaaataacgt
    35581 taccgaaggc tttatcgctg gtcgtctcac tcgcgaactg ctggcaggtg tacctgactt
    35641 agcttcacgt accgtgatga cctgcggccc ggctccgtat atggattggg tagagcagga
    35701 agtgaaagcg ctcggcgtga cgcgtttctt taaagagaaa ttcttcaccc cagtagcaga
    35761 agcggcgacc agcggtctga aattcaccaa actgcaaccg gcacgagaat tttacgcccc
    35821 ggttggcacc acgctactgg aggcgctgga aagcaataac gttccggttg tcgctgcctg
    35881 ccgtgcgggt gtttgcggct gctgtaagac aaaagtggtt tccggtgaat atacggtgag
    35941 cagtacaatg acgctgaccg acgccgaaat cgctgaaggt tacgtactgg cctgctcctg
    36001 ccatccgcag ggggatttgg ttctcgcata atcgccttat gcccgatgat attcctttca
    36061 tcgggctatt taaccgttag tgcctccttt ctctcccatc ccttccccct ccgtcagatg
    36121 aactaaactt gttaccgtta tcacattcag gagatggaga accatgaaac aaacggttgc
    36181 agcttatatc gccaaaacac tcgaatcggc aggggtgaaa cgcatctggg gagtcacagg
    36241 cgactctctg aacggtctta gtgacagtct taatcgcatg ggcaccatcg agtggatgtc
    36301 cacccgccac gaagaagtgg cggcctttgc cgctggcgct gaagcacaac ttagcggaga
    36361 actggcggtc tgcgccggat cgtgcggccc cggcaacctg cacttaatca acggcctgtt
    36421 cgattgccac cgcaatcacg ttccggtact ggcgattgcc gctcatattc cctccagcga
    36481 aattggcagc ggctatttcc aggaaaccca cccacaagag ctattccgcg aatgtagtca
    36541 ctattgcgag ctggtttcca gcccggagca gatcccacaa gtactggcga ttgccatgcg
    36601 caaagcggtg cttaaccgtg gcgtttcggt tgtcgtgtta ccaggcgacg tggcgttaaa
    36661 acctgcgcca gaaggggcaa ccacgcactg gtatcatgcg ccacaaccag tcgtgacgcc
    36721 ggaagaagaa gagttacgca aactggcgca actgctgcgt tattccagca atatcgccct
    36781 gatgtgtggc agcggctgcg cgggggcgca taaagagtta gttgagtttg ccgggaaaat
    36841 taaagcgcct attgttcatg ccctgcgcgg taaagaacat gtcgaatacg ataatccgta
    36901 tgatgttgga atgaccgggt taatcggctt ctcgtcaggt ttccatacca tgatgaacgc
    36961 cgacacgtta gtgctactcg gcacgcaatt tccctaccgc gccttctacc cgaccgatgc
    37021 caaaatcatt cagattgata tcaacccagc cagcatcggc gctcacagca aggtggatat
    37081 ggcactggtc ggcgatatca agtcgactct gcgtgcattg cttccattgg tggaagaaaa
    37141 agccgatcgc aagtttctgg ataaagcgct ggaagattac cgcgacgccc gcaaagggct
    37201 ggacgattta gctaaaccga gcgagaaagc cattcacccg caatatctgg cgcagcaaat
    37261 tagtcatttt gccgccgatg acgctatttt cacctgtgac gttggtacgc caacggtgtg
    37321 ggcggcacgt tatctaaaaa tgaacggcaa gcgtcgcctg ttaggttcgt ttaaccacgg
    37381 ttcgatggct aacgccatgc cgcaggcgct gggtgcgcag gcgacagagc cagaacgtca
    37441 ggtggtcgcc atgtgcggcg atggcggttt tagcatgttg atgggcgatt tcctctcagt
    37501 agtgcagatg aaactgccag tgaaaattgt cgtctttaac aacagcgtgc tgggctttgt
    37561 ggcgatggag atgaaagctg gtggctattt gactgacggc accgaactac acgacacaaa
    37621 ctttgcccgc attgccgaag cgtgcggcat tacgggtatc cgtgtagaaa aagcgtctga
    37681 agttgatgaa gccctgcaac gcgccttctc tatcgacggt ccggtgttgg tggatgtggt
    37741 ggtcgccaaa gaagagttag ccattccacc gcagatcaaa ctcgaacagg ccaaaggttt
    37801 tagcctgtat atgttgcgcg caattatcag cggacgcggc gatgaagtga tcgaactggc
    37861 gaaaacgaac tggctaaggt aaaaagggtg gcatttcccg tcataataag gacatgccat
    37921 gattgattta cgcagtgata ccgttacccg accgagccgc gccatgctcg aagcgatgat
    37981 ggccgccccg gttggggacg acgtttacgg agacgaccct accgttaatg ctctgcagga
    38041 ctatgcagca gagctttccg gtaaagaagc cgccattttt ctgcctaccg gcactcaggc
    38101 caacctggtc gctctgctca gtcactgcga acgtggcgaa gagtatattg tcggtcaggc
    38161 cgcgcataac tatctgtttg aagccggtgg cgcagcggtg ctgggcagta ttcagccgca
    38221 acccatcgac gcggctgccg acggcacgct accgctggat aaagtggcga tgaaaatcaa
    38281 acccgacgat atccatttcg cccgcaccaa attactcagt ctggaaaaca cccacaacgg
    38341 caaagtgctg ccgcgtgaat acctgaaaga agcatgggaa tttacccgcg agcgcaatct
    38401 ggcgctgcat gttgacggtg cgcgcatctt taatgccgtg gtggcttacg gctgcgaact
    38461 gaaagagatc acgcaatatt gtgattcgtt caccatttgc ctgtcgaaag gtcttgggac
    38521 gccagtcggt tcattactcg tcggtaatcg tgattacatt aaacgtgcca tccgctggcg
    38581 gaaaatggcc ggtggcggga tgcgtcagtc cgggattctg gctgccgccg ggatgtatgc
    38641 cctgaaaaat aacgtcgcac ggttgcagga agatcacgac aacgccgcct ggatggcgga
    38701 gcaactgcgt gaagcaggcg cggatgtgat gcgtcaggac actaatatgc tgtttgttcg
    38761 cgtcggggaa gaaaatgctg ccgcgttagg cgaatacatg aaagcgagaa acgtgctgat
    38821 taacgcctcg ccgattgtcc gcctggtgac gcatcttgac gtctcgcgcg aacaactggc
    38881 ggaagtcgcc gcccactggc gcgcattcct ggcgcgttaa ggagagagac gtgccgcaac
    38941 gcattttagt tctcggtgcc agtggctaca ttggtcagca tctggtgcgc acactcagcc
    39001 agcaagggca tcagatcctg gcggcggcac gtcatgtcga caggcttgca aagctgcaac
    39061 tggcaaacgt cagttgccat aaagtcgatc tcagctggcc ggataacctt ccggccctgt
    39121 tgcaggatat cgataccgtc tattttctgg tgcacagcat gggcgaaggc ggcgatttta
    39181 tcgctcagga gcgccaggtg gctctcaacg tccgcgatgc gctacgtgaa gtaccagtta
    39241 agcaattaat ctttctcagt tcgttgcagg ccccgccaca tgagcagtcg gatcatctgc
    39301 gtgctcgtca ggctacggcg gacattcttc gtgaagcgaa tgtacctgtg accgaacttc
    39361 gggccggaat tatcgttggc gcaggttcag cggcgttcga agtcatgcgc gatatggtct
    39421 acaacctgcc agtgttaacg ccgccacgct gggtacgttc acgcaccacg cccatcgcgc
    39481 tggaaaactt gctgcactat ctggtggcgt tgttagatca tccggccagc gaacaccgca
    39541 tcttcgaagc cgccggacca gaggtgctca gttatcagca acagtttgaa cattttatgg
    39601 cggtgagcgg taagcgccgc tggttgatcc ccatccccct ccccacccgc tggatttcgg
    39661 tgtggtttct caatgtgatt acttccgtac cgcccaccac cgccagggcg ttgattcagg
    39721 ggctgaaaca cgatctgctg gcggatgata ccgcgctacg tgcactcatc ccacaacggc
    39781 tgatcgcttt cgatgacgcg gtacgtagca cgttgaaaga ggaggaaaaa ctggtcaact
    39841 ccagcgactg gggctacgac gctcaggcct ttgcccgctg gcgaccagag tacggttatt
    39901 ttgccaaaca ggcggggttt accgttaaaa cgtccgccag ccttgctgct ttatggcagg
    39961 tagtgaacca aatcggcggt aaagagcgtt atttctttgg caatattttg tggcagacac
    40021 gggcgttgat ggaccgcgcg atcggtcata agctggcgaa aggccgcccg gagcgcgaat
    40081 atttacagac tggcgatgcg gtggatagct ggaaagtgat tgtcgttgaa ccggaaaaac
    40141 aacttacgtt gttatttggc atgaaagcgc cggggctggg acgactgtgt tttagcctgg
    40201 aagataaagg cgactatagt actatcgatg tccgcgcttt ctggcatccg cacggtatgc
    40261 cggggctgtt ttactggtta ttgatgatcc ccgcgcatct gtttattttt cgcggaatgg
    40321 caaaacaaat cgccagactg gcagaacaaa gcacagatta atcgacaaaa cctgcgttat
    40381 tctttcatgt ttcccattgc atcatggggg aaatcacgga agaatgcgca cgttattttc
    40441 tcaacaacag tggattgata tgaaggtact ggttaccggc gccaccagcg gcttaggtcg
    40501 aaacgcggta gagtttttat gccagaaagg catcagcgtg cgagcgaccg gtcgcaacga
    40561 ggcaatgggc aaattgctgg aaaaaatggg cgcagagttt gttccggcgg atctgaccga
    40621 gctggtttca tcacaagcta aagtgatgct cgcgggcatt gatacgctgt ggcactgctc
    40681 cagctttacc tcaccctggg ggacacaaca ggctttcgat ctggctaacg ttcgcgccac
    40741 tcgccgcctg ggtgaatgga ctgtcgcctg gggtgtgcgt aactttattc atatctcttc
    40801 cccctccctg tacttcgatt atcaccacca tcgcgatatt aaagaagatt ttcgccctca
    40861 ccgcttcgcc aacgagtttg cccgcagcaa agcagccagc gaagaagtga tcaatatgct
    40921 ttcgcaggcg aatccacaaa cgcgctttac tattctgcgc ccacaaagtc tgttcggacc
    40981 gcacgataaa gtctttattc cccgtctggc gcatatgatg caccactacg gcagtattct
    41041 gttaccgcat ggcggcagtg cgctggtcga tatgacctac tatgaaaatg ccgtgcacgc
    41101 aatgtggctg gcaagccagg aagcctgcga taagctacct tccgggcgtg tgtacaacat
    41161 caccaacggc gagcatcgca cactgcgcag catcgtgcag aagctgatcg acgagttgaa
    41221 tattgactgt cgtattcgtt ccgtccccta cccgatgctg gatatgatcg cccgcagcat
    41281 ggagcgttta ggccgcaagt cagcaaaaga gccgccgctg acccactacg gcgtctccaa
    41341 gttgaatttt gactttacgc tggatattac gcgtgcgcag gaagagttag gttatcagcc
    41401 ggtcatcacc ctggatgaag gtatcgagaa aactgccgcc tggctacgcg accacggaaa
    41461 actgccgcgc taatcctgcc cgtatttctc cagcaatgct tcggcaatcg cctgagtttc
    41521 tgcatccgct tcgccgttat ataacgtcgg gcggaaatgc atctggaatg ccataatcac
    41581 gcgccgctgc tcgcgcggtg tcatatcagg tttaacgtca taaccgtagc gcgccaaaag
    41641 ctccagcaat gacgcggtat ctaccggtgt gtgcggcgcg cgcccggcaa ggtaaaagtt
    41701 aacccgctgc gcatccggcc aggcaccaat cccctgctgc gccagttgct gccagggaaa
    41761 taatggcccc ggatcgtctt tgcgctgcgg tgcgatatcc gcatgtgcca ctacgttttc
    41821 cggcttgatg tgataacggg caataatatc tttcgccagt ggaataagcg cctgaatctg
    41881 tgccggttca aacggggcaa aatatttcac tccggccgat ttttgccagc cacggttttc
    41941 cagctcaatg ccaatagagg tgtcgttaag gcgcgttgcc ccgcgccagg cgctaatccc
    42001 cgcatgccag gccagttctt gttccggcac cagttgccag atgcgcggtt taccgttgta
    42061 tcgcggtggt accgcaggga ccagataatg cgagctgacc tgcttatcgg tcagtgtcgc
    42121 cagcgagcta tcaaaatcat ctgcggtgta gtggatcacc agcactttaa tgcgcggata
    42181 cgccgcctgc gcctggcgtc gggtatcaag ctgatatccc tctttctcga caatgccttt
    42241 ttcgcctgca caccctgcca gtaacagagc ggcagcgatc agccagaaga ctcttctcat
    42301 cgacgcgttt tcaccgccgt accgctgacg ctaaccatca gcatactgcc gttttgcccg
    42361 accgtttcgt agtcgatatc aataccgacg acggcatcgg cccccagcgc ccgcgcctgg
    42421 gagcctaatt cctcaaaggc gatctcccgt gctttacgca gttctttttc atacgcaccg
    42481 gaacgtccgc caacgatatc gcggataccg gcaaagaaat cacggaaaat attggcacct
    42541 aaaatcgctt cgccggtcac cacaccgcaa tattcaacga tgatctggcc ttccagagtt
    42601 ggggttgtag aaaattgcat gatcgatctc cttgtttgtt aagccttcga tctctaaagc
    42661 attatcagac tgatacgcta ttattgaaag gatatcatta ttatcgtaga acgctttcag
    42721 agcgatcgct tataaggaaa tcattatgcg ctacagcaaa ttgacaatgc ttatcccctg
    42781 cgcactgctc ctcagtgcct gcaccacagt cactccagct tataaagata acggcacacg
    42841 cagtggtcct tgcgttgaag gaggcccgga taacgtggcc cagcaatttt atgactaccg
    42901 cattttgcac cgcagcaacg acatcaccgc cttgcgccct tatctgagcg acaaactggc
    42961 gacactgctt agcgatgcct cccgggataa taaccatcgg gaactactga ccaacgatcc
    43021 attctccagt cgcacaacct tgccagagag cgcccgtgtt gccagtgcct caacaatccc
    43081 gaatcgtgat gcgcgaaata ttcctctgcg tgtggatcta aaacagggtg atcagagctg
    43141 gcaggatgaa gtgttaatga ttcaggaagg ccagtgttgg gtgatcgacg atgtacgcta
    43201 tttaggcggc agcgttcatg ccacggcggg tacgctccgt cagtccatcg agaatcgcta
    43261 acgccttgat caaccaataa accccgtaaa ttgtccggca tttcgtgagg aatgccgaca
    43321 tttatgctcg ccgaccaccg cccccgttat tttgtgctat gtttattgaa taatgcgctt
    43381 tgcttttaac ttttaaagca gaaatattgc ataattattc tgtcaaaggt actatcttcg
    43441 acctcaatag ctatcagact gccagtatac gagtgtcaat gagtattcaa ttaaacggca
    43501 ttaattgctt ctacggcgcg catcaggcgc tgttcgatat cacgctggat tgcccacagg
    43561 gcgaaacgct ggtgttactt ggccccagcg gcgcgggtaa aagctcgctg ctgcgtgtac
    43621 tcaatctgct tgagatgccg cgctccggta cgctcaacat tgcaggcaac catttcgatt
    43681 tcaccaaaac accctctgac aaagcgattc gcgatttgcg tcgtaacgtt ggcatggtgt
    43741 ttcagcaata caacctgtgg ccgcatctga ccgtgcagca aaacctgatt gaagcgccct
    43801 gccgtgtact ggggttgagt aaagatcagg cgctggcccg tgcagaaaaa ctgctggaac
    43861 gtctgcgtct caaaccttat agcgatcgtt acccgctgca tctttctggt ggtcagcagc
    43921 agcgtgttgc tattgcccgt gcgttgatga tggaaccgca ggtactgctg ttcgatgaac
    43981 cgaccgccgc actggacccg gaaattacgg cacaaatcgt cagcatcatt cgtgagctgg
    44041 cagaaacgaa tattacccag gtgatcgtca cccacgaagt tgaagtggcg cgtaaaaccg
    44101 ccagccgagt ggtgtatatg gaaaatggtc atatcgtaga acaaggcgac gcgagctgct
    44161 ttaccgagcc gcaaaccgaa gcatttaaaa actatctctc tcactaagat tcgggataac
    44221 aacaatgaaa aaagttctga ttgccgcgtt aattgcaggt tttagtcttt ccgccacagc
    44281 tgccgaaacc attcgttttg ctaccgaagc atcctatcct ccgtttgaat cgattgatgc
    44341 aaacaaccag atcgttggtt ttgacgtcga cctggcacaa gcgctgtgta aagagattga
    44401 tgcaacctgc actttctcta accaggcgtt tgacagcctg atcccaagcc tgaaattccg
    44461 tcgcgtagaa gccgtgatgg cgggcatgga tatcactccg gagcgtgaaa agcaggtgct
    44521 gtttaccacc ccgtactatg acaactctgc cctgtttgtg ggtcagcaag gcaaatacac
    44581 cagtgttgat cagctgaaag gcaaaaaagt cggcgtacag aacgggacga cacaccagaa
    44641 attcattatg gataagcacc cggaaatcac taccgttccg tatgacagct accagaacgc
    44701 aaaactggat ctgcaaaacg ggcgtatcga cggcgtcttc ggtgacaccg cagtggtcac
    44761 tgagtggctg aaagataacc cgaaactggc ggcggtgggc gacaaagtga ccgataaaga
    44821 ttacttcggc actggcctcg gcatcgcggt acgtcagggc aacactgagc tgcagcagaa
    44881 actcaacact gcgctggaaa aagtgaagaa agatggcact tacgaaacca tctacaacaa
    44941 atggttccag aagtaattcc tgatgaatga attttttcct ttagcaagcg ccgccgggat
    45001 gaccgtcggc cttgccgttt gtgcattgat tgtcgggctg gcgctggcga tgttctttgc
    45061 ggtatgggag tcggcaaaat ggcgtcctgt cgcgtgggca ggttcagcgc tggtaaccat
    45121 tctgcgtggc ctgccagaaa ttctggtggt gctgtttatc tattttggct cctcgcagct
    45181 gctgctgacg ctttcggatg gcttcactat caatcttggg ttcgtgcaga tcccagtgca
    45241 gatggacatt gagaacttcg acgttagccc gttcctttgt ggtgtcatcg ctctgtcact
    45301 gctgtatgcc gcctatgcct cgcaaacgct tcggggcgcg ttgaaagcgg tgccggtggg
    45361 tcagtgggag tctggtcagg cgctggggct gtcgaaatcg gctatctttt tccgtctggt
    45421 gatgccgcag atgtggcgtc atgcgctgcc tggcctcggt aaccagtggc tggtgctgct
    45481 gaaagatacc gcgctggtca gtttgattag tgtgaatgat ttaatgctgc aaacaaaaag
    45541 catcgctact cgtacccagg aaccatttac ctggtacatt gtggcggcgg cgatttacct
    45601 ggtgatcacc ctgctcagtc agtacattct caaacgcatt gacctgcgcg cgacacgttt
    45661 tgagcggagg cccagctaat gtttgagtat ttacccgaac tgatgaaagg gctacacacc
    45721 agcctgacgc taaccgttgc ctcgctgatt gtggcactga ttctggcatt gatttttacc
    45781 atcatcctga cgctgaaaac gccggtgctg gtgtggctgg tgcggggtta tatcacgctg
    45841 tttaccggta cgccgctgct ggtgcagatc ttcctgattt attacgggcc gggccagttt
    45901 ccgactttgc aggagtatcc ggcactgtgg catttgttgt cagaaccgtg gttatgtgcg
    45961 ctgattgcgt tatcgctgaa cagtgcggcg tataccacgc agctgtttta cggtgcaatt
    46021 cgtgcgatcc cagaaggtca gtggcagtcc tgtagcgccc tgggaatgag caaaaaagat
    46081 acgctggcga tcctgctgcc gtatgccttt aaacgctcgc tctcttctta ttccaacgaa
    46141 gtggtgctgg tgttcaaaag tacctctctg gcttacacca ttacgctgat ggaagtgatg
    46201 ggatacagcc agttgttgta cggacgcacc tacgatgtaa tggtgttcgg tgcggcaggg
    46261 attatttacc tggtcgtcaa cggcctgctg acgctgatga tgcgtctgat cgagcgcaaa
    46321 gcactggcat tcgaacggcg aaattaatgt agctgcgtac attcgccggg ggggtttgta
    46381 ggtctgataa gacgcgccag cgtcgcatca gacatcagca cggtgcctgt gccggatgcg
    46441 gcgtggacgc cttatccggc ctacatagtg cataaattct tataatgaag acggacaact
    46501 cactaaggta tccgtctttt tttatttcat ttaaattatt taatcatatt tattgcatat
    46561 aaattcactt gatggcattg ttatcccatg ccgcagacac ggccaaaaat cataagattg
    46621 acagacggga gttccatcat gaaaaagtta gttcttgccg ctttacttgc ttcctttact
    46681 ttcggtgctt ctgccgcaga gaaaatcaat tttggcgttt cagccaccta tccacccttt
    46741 gaatctatag gtgctaataa tgagattgtc ggctttgata tcgatctggc aaaagccttg
    46801 tgcaaacaaa tgcaggcaga atgtactttt actaatcacg cgttcgacag cctgatcccg
    46861 tcactgaaat tcagaaaata tgacgccgta atctccggta tggatatcac gcccgagcgt
    46921 agcaagcagg tatcgtttac cacgccctac tatgaaaact cagccgtcgt gattgccaaa
    46981 aaagatacct acaaaacgtt tgccgatctg aaaggcaaac gtattgggat ggaaaacggc
    47041 accacgcacc agaaatatat tcaggatcag caccccgaag tgaaaactgt ctcctatgac
    47101 agttatcaga atgcctttat cgatctgaaa aatggtcgta ttgatggggt atttggtgac
    47161 acagcggtgg taaacgaatg gctgaaaacc aatccgcaac tgggcgttgc tactgaaaaa
    47221 gtgaccgatc cgcaatactt cggcaccggt ctgggcatcg ctgtacgtcc ggataacaaa
    47281 gctctgctgg aaaaactgaa taacgcgctg gcagcaatta aagctgacgg tacatatcag
    47341 aaaatcaatg accagtggtt tccgcagtaa ttgagactga tgacaaaagt aagattgcct
    47401 gatgcgctgc gcttatccgg cccacatcaa ctctgcaatg tattgattta gcatgatttt
    47461 gtaggccgga taaggcgtac acgccgcatc cggcatgaac aaagcgcacc tgatcagcaa
    47521 tctgaagccg cacctgcggc ttttttattg cttcaccagc agcgtcagca cttcatagtg
    47581 cgcggtgtgc gggaacatat caaaaagctg tacccgttcg atgcggtaac ctggcagttc
    47641 gcggatatct ttcgccatgg tttgggcgtt acagctggag tagataataa aacgcggtgc
    47701 cattgttgag agataatcac atagcggttt accaatgccg cggcgcggcg gattaaccag
    47761 caccagctcc ggcacctccc cctgagcggt ggcaaactga gtggagtcca gcgcctgaaa
    47821 ttgcaaacgc gttaagccca gttcagcggc tgactgcttt gcacaggcaa tggcctctga
    47881 tgcaatttcg atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac caaagccacc
    47941 cacaccgcag aacagatccc acatatgttt aactggcagc tgccgtaccc agtcgcgcgc
    48001 ggtagcgtac agctggctgg cgaccgccgg attagtctgg aagaaacttt gcggacggat
    48061 ccacagcggt acgtcattaa aacgctctgc cagcgcctgt tgttcagtaa ggtagatctc
    48121 cgtctcccct tccataatcg ccatatgtac cggctgaata ttgacggtaa taactttcag
    48181 ttgcggtagt tgttcctgta accacggcag cgccttacgc agttgtgcca gtttggtttc
    48241 agaacgcagt acaaagcgca gcatcatgcc gccatcgctc tggctttcag tcagcagaat
    48301 gtatttcagt tcgccacgct tacgcgccac gttgtagggt gtcagccccg cacgggcgat
    48361 aaacggtttt agcgccgcaa aaacgggcgc aaatgaggca ggataaagcg ggcagtcaca
    48421 aaggtcttct ggtgtgccgt cgcgatgcag cataccgagc agtggttttt caacgctacc
    48481 actcaccacc attttggctt tattacgaaa cccttgttcc gggcctgaca ccggcgcgca
    48541 ccattcctca accggaaaat cggcgagcag atttttaaga tcggcggttt tagcggagag
    48601 ttgctctgga atcggctgcg ttatccactg acaggaacga cagcgacccg cgtcgtaaag
    48661 tgcgcactgc atacattgac cttcacatca tcaggggcga cgattataca cattattgca
    48721 actggaagaa ccgccgactg gtcgagggaa cgaacagcag catcaggatg agcatatccg
    48781 gcagcttctg cagcatcagg ctatggaaga tttcacgttt tgattcaccg ggaatgctga
    48841 acagctccgg ataaccgtac cccagcgaag ccgcccacaa gtaacttgcg gcggtgattt
    48901 gtgtcagcag atatagccag cgcgcccagc gacgcccttt caccagtgaa aacgcacacc
    48961 agatctcaat gaaaaccagc accagactgc ttaaaaagac cagcgttaaa ctccaggttt
    49021 gtacgctgcg atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg aaaatcatca
    49081 gcacgtcgag gccacggatc ataataatgg cgagcgccgc cacctgcacc agcgcaggca
    49141 cattcaggcg agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt tcgtcttcca
    49201 tgaaaacaat gccgcggcat gcgcggcatt atgtagccag gttggcaaat tttagtgtct
    49261 tcagccatgt cttgcacgct ggatatcgcg tatccgctgt ttttctgcgc gagccatcag
    49321 ataccaggcg ataaatccga caattccgac cgcaccgaga attaacgttg caagggcgtt
    49381 gatttccgga ttcaccccca tccgcacgct ggaaaagacc agcatcggta acgtggtggc
    49441 tcccggcccg gaaacaaagc tggcgatcac cagatcatca agcgacaaag taaaagccag
    49501 taaccagcca gaaatgatcg cgggcatgat catcggtagc gtaatgacga aaaacacttt
    49561 cagcggcgtc gcaccgagat ccatcgctgc ttcttctatc gagcgatcca gttcccgcaa
    49621 tcttgaagaa ataacgaccg ccacataagc cgtacaaaaa gtgacatgcg ccagccagat
    49681 ggtgagcata ccgcggtccg caggccagcc aatggcatga gcaagcgcga cgaataataa
    49741 caacagcgac aagcccgtga tgacatctgg catcaccagc ggcgcggtga tcataaaggc
    49801 aaagccattt gatccgcgaa acctgccaaa gcgcaccagc accaccgccg caatagtccc
    49861 gaggatcgcc gccgccgttg ccgcacaggc agcaattgtc aggcttaaac caaccgcact
    49921 catcatcgcg tcatcgcgca ataactcacc ataccagcgc gttgaccagc cggcccacac
    49981 cgtcaccagc ttcgagctgt taaacgaata gataaccagc atcagcattg gcgcgtagag
    50041 aaaggtaaag cccagcagca aaatcacaat ccgccagggc gaacgaacta ccggtaaatt
    50101 attcatccgt gttctcccac gcttttttgc tggtgtttgt ggaaccacat tatcggcaca
    50161 attagcagca gcaacatgat gatcgctacc gccgaggcca ccggccagtc gcggttattg
    50221 aaaaactctt gccatagcac gcgcccgatc atgatgctgt ccgggccacc gagcagttcc
    50281 gggatcacaa actcgcccac agccgggata aacaccagca tcgatccggc aataatccca
    50341 cctttagtca gcggcacgat cacagtaaag aacgttttca gcggtcgtgc accgagatcc
    50401 agcgctgctt ccaccagcga ataatcaata cgaatcaacg cggtataaat cggcagtacc
    50461 ataaacggca cgtaggcgta aacaatgcca atataaactg ccagattggt atgaagaatg
    50521 gtcagcggtt gatcgataac ccccagccac agcagaaaat tattcagcac accgttgttt
    50581 tttaatattc ccatccaggc ataaacgcgg atcagaaacg aggtccacga cggcaggatc
    50641 accagtagta ataaaatatt acgggtcgaa ggcttactgt gcgccaccgc ccacgccagc
    50701 ggatagccga tcagtaaaca gcaaaatgtc gaaatcgcag ccacctgtag cgactggaga
    50761 taagcatcga aatagagcgg atcgtcggtc agttgcagaa aattaccgag attaagagtg
    50821 atggacagtt gcccgtcagc ccactccatc agttcggtat aaggtggaat agcgcgcgcc
    50881 atctccgcca ggcttatttt aaagacaatc agaaatggca gcagaaacag caagatcaac
    50941 cagatatatg gcaacgcaat gaccagtttg cgcccatgct tcatttgcag ctgcgacaac
    51001 cacagcttaa atccgcccgg cggtttcgac tgggcagcag gttcaagtgt attcatcttt
    51061 gctccttaaa ccgtcagcac cacacagctg tccacttccc agcacaaacg cacttcgtcg
    51121 ccccaggtcg gtaacccttt acgatgacga tgggcgtttt gtagctgggc gctaatcatc
    51181 tgcccacttt tcagacgaac gtgatacacc gaaagatcgc cgagataggc aatgtgtatc
    51241 acctccccca ccgcgaagtt acaaccattg gcgggcggct cttcgcaaag catgattttt
    51301 tccgggcgca gcgctacgtg taccggcacg ttatcgacca ctgaagcatc cgcatcgact
    51361 ttcagtggat gcaccagccc cggcgaatca agcaccaggc catcttcctg acgctctttg
    51421 agtacacctt caaagacatt aaccgaaccg ataaattcag cgctgtagcg ggtagttgga
    51481 tgctcgtaga tctcttccgg ctcgccaatc tggacaaatt tcccgcgatt cataatggcg
    51541 atacgccccg ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac acaagtcaca
    51601 ccgacgcgct ccagaatatc caccacttca agctgcatcc ggtcacgcag ctttttatcc
    51661 agcgcgccca tcggctcatc gagcagtaat agtttcgggc gtttggcaag gcttcgggcc
    51721 agggccacac gctgtcgctg accaccggaa agctgatgcg gtttgcgttt ggcgaactcc
    51781 tgcatgtgca ccagcccaag catctcgttg acccgggtgg caatttccgc tttcggtagt
    51841 ttgtcctgtt tcaggccaaa ggcgatgttc tgctccacag tcatatgggg aaacagcgcg
    51901 taagactgaa acatcatatt gatggggcgc aggtaaggcg gcacctgtga caaatcgacg
    51961 ccatcaagca ttatctgtcc ggcagaaggt tgttcgaaac ctgccagcat acgcagcagc
    52021 gtggacttgc cacagccgga tgcgcccagc agcgcgaaga tttcaccttt gtagatggtc
    52081 aggctgacat catccaccgc atgttgacca tcgtaggatt tggtgaggtt gcggatttct
    52141 aatagcggca tcagcgcctt acgggttttc gcctgcgggc gagggatagc gtcattcacg
    52201 gctgctctcc ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt gccggcgggt
    52261 gtggtgcgcc cgcccggcat ctaaggctgc ggattatttt ccgctcttca ctttggtcca
    52321 cgcgcgggtg cgcacacggt cgattttcgg gtcctgcact ttcagagtga acagcttcgc
    52381 acgaacatcc gcaggcggat aaatacccgg gttctcacgg acttccgcac tcaccagcgg
    52441 cgtggctgct ttattggcgt tggcatagaa cacattgtcg gaaatatgcg ctactacatc
    52501 cgggcgcagc aggtaattca ggaactgata ggcttcgtct ttgtttttgg catccgcagg
    52561 catggcgaat acatcaaaga acgccatcgc cccttctttt ggaatcgaga acgagacatt
    52621 cactccattc ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcac ctgcccagcc
    52681 gatagcgacg caaatatcgc cgtttgccag gtcgttaatg tattgagatg aatggaaata
    52741 acgaatgttc gggcgcagct ttaacagcag atcggttgcc ggtccggtgt aatcatccgc
    52801 tttagtgctg ttgggatctt tgccgagata attcaacacg gtagcaaaaa cttcttctgg
    52861 cgcatccagg aaagagacac cgcagctttt cagtttttcc agattttcag gtttgaggat
    52921 caagtcccag ctatcgacgg gcgcgttttc gcccagcaca gctttaactt tatcaacgtt
    52981 atagccaatc ccggtcgtcg cccacatata gggcatagca aatttattgt cgggatcgtg
    53041 tttggcgacc agcttcagca gctccgggtc gagattcttc cactccggca atttgctttt
    53101 gtccagcggc tggaaaactc ccgcagtcaa ctggcgttca agaaagctgg cagatggaac
    53161 caccaagtca aagccggtgc tacccgccat taatttgcct tccagtactt cgttagagtc
    53221 gaaaacatcg tagacgactt taataccggt ttctttttca aaattggcca ccgtgtccgg
    53281 ggcgatataa tcagaccagt tataaacgtg gagtgttttt tgttcagccg cgagcgtgcc
    53341 gacagagacg gccatcagag cacccgcagc cagacccgaa agccattttt tatttaaggc
    53401 ggtcatatct ctttccttct gaaagttcgt taacaaacga acaaaagttg tcacactaaa
    53461 agatatgcaa taaacgtgca tattttttat cggcggggtg taagaaaaga gaactctctc
    53521 ccggccagca ctgctcgcta tataaaagca tcgcaagaat aactttagcc agggtttgag
    53581 actatagctc ggattaaaaa acggtattta acaaattttt atgcgtattt gctctatgtg
    53641 ataaagccaa atcgacacaa gagaagtgaa ataatcttta agaaaaggtt aaaaacagca
    53701 gataattttg cagcgcgcag ccactgcacc agcgactgcg tgctcgaaag gagttatcag
    53761 tggaggaaat agttttcggt aacgttgttt tgcccttcat cgtcagtcgg taacagcagt
    53821 tgatgggcat tagcttcaag aatgaccatc gaaatctgct cttcgctctg acgcacgaac
    53881 catgcaaact gctcataggt tacgccctgc ataacggata aagactggca aaccaccagc
    53941 tttggcagat tatcatcctg catatcaaga aatgctttca cggtcagcga actggcattg
    54001 atggcagata aatccgccgc cagcggcaat acggctgaag gtcggacttc cgccatggca
    54061 gaaaacagga tcgtgttatc gatcagatcg attttggcat caaagacacc gtcgaaattc
    54121 tgcatatggg gcagatgcag agcctgacag ttatcacatt caaaaaaact catccccagg
    54181 tcatcgagcc attgacgcag cgtatccaga ccaggaacga ccagcgatgt cataattttg
    54241 tacaacctct ttcgataaaa agaccggcac agcttacgca aaaagcgcag gcaaaaccat
    54301 gatcagtaat gtgattgcga ctaaccaccc gttttcaggc aatattctgt cgtagcgtgg
    54361 cgttcgatcc agcggatcat tttacccgcg atgtcgatac cggtggtttt ttctattcct
    54421 tccagccccg gcgacgcatt cacctccatc accaacggcc cgcgattagc acgcagaata
    54481 tccacaccag caacgtccag cgccatcgtt cgcgcggctt ttatcgcgat ttcacgctcc
    54541 tgtggtgtga tactggcgac gcttgccgcg ccgccacgat gcaaattgga acgaaaatcg
    54601 ccctctttcg cccgccgttc aatcgcagcg accacttcat cgccaacaac cagacagcgg
    54661 atatcgcacc cttgcgcctc tttgatatat tcctgcacca gaatatgcgc gttcagaccg
    54721 cggaaagcgt caatcacgct ttccgccgcc tgacgcgtct ccgccagcac gacgccaatt
    54781 ccctgcgtgc cttcaaccaa cttgaccacc agcggcgcac caccgaccat gtcgattaaa
    54841 tcgctggtat catccggcga atgcgcaatg cccgtgacag gcaggtcgat gccctgacgc
    54901 gccagcagtt gcatggaacg caatttgtca cgcgcccggg caatggcgac cgactcattg
    54961 agcggatagc tccccagcat ctcgaactgg cgcagtgccg ccgtcccata aaaggtaatg
    55021 gcggtgccaa tacgcgggat cactgcgtca aaatggggta acttgcggcc tttgtagtga
    55081 atagaagacg ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag aatttcaacc
    55141 aggtgaccgc gctgtatagc ggcttcacgc agccgcttac acgaatagag cgttccatcc
    55201 cgggacaata tggcaatttt caccctgcac ctctctgtca gacctggtca aaagcccgcg
    55261 tatcatacat cgacgtggcg gttttagcgc gtcgcccaac cctgtttgtg caaataatcc
    55321 agaataaatg ggcggctttc tttaatgatt gttcggcgga tatgatcgct ccaggtatcc
    55381 cggcgattat tgctgccacg ggtgaggtaa tattccgcca gttgctcgtc atactgcgcc
    55441 agtgcgcctt tatccagcgg ttgatagctg ttttcatgca ccaaaatgga ggccggtaaa
    55501 cgcggcttaa gatccggatt atccgcaggc cagccaaggc acagcccaaa cagcggcaga
    55561 acatgctgcg gtaatttaag cagtttcgtc accgcttcaa tattattgcg caggccgccg
    55621 atatataccc cgcccaatcc cagcgattcc gctgcgatta atgcattctg cgccatcatt
    55681 gccgtatcaa cgacaccgag caacagttgt tccgccaggc cgagctgagc atccggacag
    55741 atctgtaaat ggcggttaaa gtcggcacag aacacccaga actccgccgc ttgcgctacg
    55801 tgtttttgcc cgccggtcag cgtcaccagt tcttcacgta acgctttgtc ggtaatgcga
    55861 ataatgctac tgcactgcaa aaaactggaa ctggacgtcg cacgggcgct gttaataatc
    55921 gcctcacgct gcgcttcgga aatgggttca tcagtgaaat ggcgaatgga gcgatggcca
    55981 caaataagtt caatggttgg cgtcattatc tttttctctt tctgaacgtg aatattgcgg
    56041 tggacggttc atcagctgtg gggcaagacg ttttgccacc tgaagaataa ccaccaccgc
    56101 agcgggaagc atgagcaaaa caccgagaaa aatcatcaga atctgcactt ctggccgaga
    56161 aaatggctca ggcagcgaca gggagtcgct taccgacagc agcgccaccg ccagtagcat
    56221 cattccgata aattccagta tcaacacgcc tttaggcaat ttaccgatcg cgcgcatacg
    56281 cttccctctg caaagtgagc cttcagtcta aaacttttca ctgtattgtg tttaacagtt
    56341 atagctttta gcaattaatg caacaggtta aacctacttt cagcgaatac attttagcgt
    56401 gatcattaca ggcataaatc tatgaggaga gaaataatgc aaaccgttat ttttggtcgt
    56461 tcgggttgcc cttactgtgt gcgtgcaaaa gatctggctg agaaattgag caatgaacgc
    56521 gatgattttc agtatcagta tgtagatatt cgtgcggaag ggatcactaa agaagatcta
    56581 caacaaaagg caggtaaacc cgtagaaacc gtgccgcaga tttttgtcga tcagcaacat
    56641 atcggcggct ataccgattt tgctgcatgg gtgaaagaaa atctggacgc ctgatcgtct
    56701 gacaagccct cgcgttgagg gctttactga ttttttctgt gctgtggttt aaacaaacta
    56761 ctgataaata agaaacacag tgcccccagc gcacaccaga acaccgcgct tagtaaccat
    56821 gccagctctt gccagaatga gcgcgtcggt gaaaaaaaca gccgcataat gagcattgaa
    56881 cagggtgccg ccagcattgc gccaaataga ggtttcagga cttctctacg ctgtgaaaag
    56941 aagctggcga ctgctccagg aagaatgaaa aatagcaagc cgatttcagg atgcccggca
    57001 gcccgaaaag cgcctttcat gtgcgtcgcc agaaaaaggc acaccacaat gaagaggaca
    57061 aaacagcaga ttgcccccgc ccaacgttgt ttatgtttca ctcgttcctc ctgacactgc
    57121 gtctatcgaa cacatttttc gccagtgtgg cgttcagtaa gataaagccg cttcgcattc
    57181 catgctaata taggccaacg caattcatat agccgttgat acctaatgtg attacactag
    57241 taaaatatat tgttacttta ctatcgttta ggtgcgctga atgaatctgc gccctgaatt
    57301 ctggtaaaaa acattatcgt aaattaccat ttctttcaac agcttactag taaacaagaa
    57361 gttagcctcc gtgaatataa acgtcgccga attgttaaat gggaattaca ttctgttatt
    57421 atttgtggtc ctcgcgcttg ggctatgtct cggaaagtta cgacttggtt cgatccaact
    57481 gggtaattcc attggcgttt tagtcgtatc gctgttatta ggccaacaac atttcagcat
    57541 taacaccgat gcgcttaatc ttggctttat gctgtttatt ttctgcgtcg gggtcgaagc
    57601 cggaccgaac tttttttcca ttttttttcg cgatgggaaa aattacctaa tgttagcact
    57661 ggtgatggtt ggcagtgcgc tggtgatcgc cttagggtta ggtaagctgt ttggctggga
    57721 tattggcctg acggccggta tgttagcagg ctctatgacg tcgacaccgg ttctggtcgg
    57781 tgctggcgat acactgcgtc attccggcat ggaaagcagg cagctctcac tggcactgga
    57841 taatctgagc ctcgggtatg ccttaaccta tttaatcggt ctggtgagtt tgattgttgg
    57901 tgcgcgttac ttgccgaaat tgcagcatca ggacttacag accagcgccc agcaaatcgc
    57961 ccgcgaacgt ggcctggaca ctgatgccaa ccgtaaggtt tatttaccgg tgatccgcgc
    58021 ctatcgcgtc ggcccggagc tggtggcctg gaccgacggc aaaaatctgc gtgaactggg
    58081 tatttatcga caaaccggct gctacattga acgtattcga cgtaacggga ttctggcaaa
    58141 tccagacggt gatgccgtgc tacaaatggg cgatgaaata gcgttggtag gctatcccga
    58201 cgcccatgcc cgactcgatc ccagcttccg taacggtaaa gaagttttcg atcgtgacct
    58261 tctcgacatg cgtatcgtca ctgaagaagt ggtcgttaaa aaccataacg ctgtaggtaa
    58321 acgtctcgca caactgaagt tgaccgatca cggttgcttc cttaaccgcg tcattcgtag
    58381 ccagattgag atgccgatag atgacaacgt cgtgcttaac aaaggtgacg ttttacaagt
    58441 cagcggtgat gcccgtcgcg taaaaaccat cgccgatcgc atcggcttta tctcgattca
    58501 cagccaggtc actgacctgc tggcattttg cgccttcttt gttattgggc tgatgatcgg
    58561 gatgatcacc ttccagttca gcacattcag tttcggcatg gggaacgctg ccgggttgtt
    58621 attcgccgga attatgctgg gctttatgcg tgctaaccac ccgaccttcg gttacattcc
    58681 gcaaggtgca ttaagcatgg tgaaagagtt cggcttgatg gtgtttatgg caggcgttgg
    58741 tctgagcgcc ggtagcggta ttaataacgg cctgggcgcg attggcggtc agatgttgat
    58801 tgccggatta attgtcagtc ttgtgcccgt ggttatctgt ttcttgttcg gtgcttatgt
    58861 attgcgaatg aaccgcgcac tgttgttcgg cgcaatgatg ggcgcacgca cctgcgcgcc
    58921 ggcaatggag atcatcagtg atacagctcg cagtaacatc cctgcgctgg gctatgcggg
    58981 cacctacgca atcgccaacg tcctgctgac gctggcaggg acaatcatcg tcatggtatg
    59041 gccaggatta ggataaaact gaagttgccc tgaaaatgaa atttttttgc acaaccgcag
    59101 aacttttccg cagggcatca gtcttaatta gtgccactgc ttttctttga tgtccccatt
    59161 ttgtggagcc catcaacccc gccatttcgg ttcaaggttg atgggttttt tgttatctaa
    59221 aatttatcta ctaccctgca accctctcaa ccatcctcaa aatctcctcg cgcgatagcg
    59281 gcttacggtc ggtgacaaaa tgcagcgtca tcccctcaat aaacgcatca agcgcgcggg
    59341 cggttccggg ttcaaaccat tgttcgagcg tttgctgact gcgctgcatc cagttttgca
    59401 ttaccgtttt taatagcggt tttcggctag ccagcgcgta gagctggtac atcagctcca
    59461 tgttatccgg cgttgcaacc tgtgagctgt agatcatatc ggtgatagcc tggcatgcgc
    59521 cttgagcatc actaacatcg ctaaaaaatg cctgatattg ccgggacata atctcagtaa
    59581 aactgctgaa cgcctccagc aacaactcat caattcctga aaaatagtag gtcatcgatc
    59641 ccaacggtac cccggcaagg gtagcaattt tgcggtgcgt aacagcatgt attccgtaaa
    59701 gtttcaccgc ctccagcgtg gcctggataa ttttttctcg ccgttgcgga tcgttagcgc
    59761 gacgcatgtc atttcctctg tctgcaattg tgtacaaatg tacacaacct tgctaatgtt
    59821 gtcttccctc ttcttatttt gacgtgatct atgaccgtaa attcttcacg taatgcattg
    59881 aaacgccgaa cctgggcgct gtttatgttc ttctttttgc cgggcctgtt aatggcgtcc
    59941 tgggcaaccc gtacgcctgc tatccgcgat attctctctg tctcgatcgc tgaaatgggc
    60001 ggtgttctct ttggtctgtc gatcggttcg atgagcggta ttctctgctc ggcgtggtta
    60061 gtgaaacgct ttgggacacg taatgtcatc ctggtcacga tgtcctgcgc attgatcggg
    60121 atgatgatat taagtctggc actctggctg acatcacccc tgctcttcgc ggttggcctc
    60181 ggcgtctttg gggcaagttt tggttctgcg gaagtggcga taaacgtcga aggtgccgcc
    60241 gttgagcgag aaatgaataa aacggttttg ccgatgatgc acggttttta tagcctgggc
    60301 acgctggcag gcgctggtgt cgggatggca ctgacggcct ttggcgttcc ggcaacggtg
    60361 cacattttat tggcggcgct ggtaggaatc gcgcctattt atatcgccat tcaggcaatc
    60421 cctgacggta cgggcaaaaa tgctgccgat ggcacccagc atggtgaaaa aggcgtacct
    60481 ttttatcgcg atatccagtt gctgctgatt ggtgttgtgg tgctggcgat ggcctttgcc
    60541 gaaggttctg ccaacgactg gttaccctta ttaatggttg acggtcacgg ttttagccct
    60601 acttccggct cgctgattta tgccggtttt accctgggga tgactgttgg acgctttacc
    60661 ggcggttggt tcatcgaccg ttacagtcgc gttgccgtgg ttcgggccag tgcactaatg
    60721 ggagcgttgg gtattgggct gattattttt gtcgatagcg cctgggtcgc tggggtgtct
    60781 gttgtactct ggggactggg tgcctcgctg ggcttcccgc tgaccatttc tgccgccagc
    60841 gataccggcc ccgatgcacc aacccgcgtc agtgtggtag caacgaccgg ttatctggct
    60901 ttcctcgtcg ggccgccgct gctgggctat ctcggcgaac attatggatt acgtagtgca
    60961 atgctggttg tactggcgct ggttattctc gcggctattg tcgcgaaagc cgtcgccaaa
    61021 cccgatacca aaacgcagac ggcgatggag aatagttgat gagcattaaa ttaattgcgg
    61081 tagacatgga tggtactttc ttaagcgatc aaaaaaccta taaccgtgag cggtttatgg
    61141 ctcagtatca gcaaatgaaa gcacaaggca ttcgctttgt ggtcgccagc gggaatcaat
    61201 attatcagtt gatctctttc ttccctgaaa ttgcgaatga aattgccttt gtggctgaaa
    61261 acggcggctg ggtagtgagt gaaggcaaag atgtttttaa tggcgagctg tcgaaggatg
    61321 cgtttgctac tgtcgtggaa catttgctga cgcgcccgga agtggaaatt attgcctgcg
    61381 gaaaaaatag cgcctataca ctcaaaaaat atgacgatgc catgaaaacg gtggcggaaa
    61441 tgtattatca ccgtctggaa tacgtcgata actttgacaa cttagaagat attttcttta
    61501 agtttggcct gaatctttcc gatgaactga ttccacaagt acaaaaagca ttacatgagg
    61561 ccatcggcga tattatggtg ccagtccaca ccggcaacgg cagcatcgat ctgattatcc
    61621 ccggcgtaca taaagccaat ggccttcgcc aactgcagaa attatgggga atagacgaca
    61681 gcgaagtggt ggtctttggc gatggcggta acgatattga gatgctgcgt caggcaggct
    61741 ttagttttgc aatggaaaat gccggcagcg cggtcgtcgc agcggcaaaa taccgcgcag
    61801 gctccaataa ccgtgaaggc gtactggatg tgatcgataa agttcttaaa cacgaagcgc
    61861 catttgacca ataaaaacgg cagcacgccc ggcaacacaa tgcctgaatg aaggctgcat
    61921 aattcgcgga aattaacccg ctgcattatt tcaggcgaaa tgattatgaa aaattgtcta
    61981 ctgttgggcg cgcttttaat gggctttact ggtgtggcga tggcgcaaag tgtcaccgtc
    62041 gatgtgccga gtggatacaa agtggttgtg gttcctgatt cagtcagcgt tccacaagcg
    62101 gtcagcgtcg cgactgtgcc gcaaaccgtc tatgtggctc ctgcgccagc tccggcttac
    62161 cgcccgcatc cttatgttcg ccatcttgcc agcgtaggtg aagggatggt tatcgaacac
    62221 cagattgacg accatcacca ttaattcgag aatgcctgat cgcacaaagc aatcaggcat
    62281 ttttttaccc ttcgtgagaa tttcccatct gtttatcttt taaaaagata accatcagcg
    62341 acagccacaa aattccgttg acaaggttga agagattaaa cagtccgttg cccccgttca
    62401 gccaggcatg tttgctgatt tcaataccga cggtaaagat cagcatttgc agcattccca
    62461 tcgccgcaga aaccgtacct ttactcatat cgctggcaaa cagggttaat cgcaccagtc
    62521 ccgcattcgc cagaccaatg ccgaaagcat aaatacttaa cccggcggtc atccataaat
    62581 acgcgtgcga tgagataacc gttgccgcag cagcgaccaa cagaccaatc ataatcggcc
    62641 agccgcccat aattatcagc gaacgtacgg tgcggcgcga ggtcaaacgc gctaacaaca
    62701 agttacccgc aattaacgcc ccgaaaatag gcacttgcag caagccatat tcatagctgc
    62761 tcaactgctc gccggtaatg atgataatcg gcgactgggc gatccacgcc agcaatggca
    62821 ggctaacgaa tcccagcgcc agcgcccccg ccacaaagcg gccgttcttc agcaccagct
    62881 tatagtcacg accgagttct ttcagtgaca gtttctcgcc tatacgcgtg gcggtttcag
    62941 gcatggcttg ttgcagaccg aaaaaggaga tcgctgccaa tgcggcaaac aagacaaaca
    63001 tcccttccca gggcagcaca tggatccacg ccgcgcccac cagcggacca agtagcggag
    63061 caatcagcgc cacgttcgcc atcagcgcgg tgatcttgat acaaaccgcc tcttcgaagg
    63121 attcctgaat tgcggcgtat cccacagcgc caatgaaaca gaggcttatg ccctgcaaga
    63181 agcgcaacag agtgaattgt tcaatgtttt gcgccagtaa tattgccaga caggtgatga
    63241 taaaccacac cacccccgcc agcatcaccg gacggcgacc aatacgatcc gacagcggcc
    63301 ccaggagcca ttgtaaaaac atcccgcccg ccagatatgc ggtcattgaa gtaggaaccc
    63361 aatcaatgcc cgcctgatat tgttccacca cggccaacat accgggttga atcatatcgt
    63421 tgccgatata ggttgaaaat tcgtaaagca ccagacagag agggaaaagt aacgcctgac
    63481 gtccaagcct ggcaccggaa gctaatttat tttgcatgca atttcttcgc caataataat
    63541 cgcgcagagt ttaataaaag cacagctaac gagaaagcga attttgtagc tgaaaccacg
    63601 gttaagcaca ttcttacatt attgcgagta tagctacgct ttctttaagt tttatttaac
    63661 ctatgcccgt tacaattacc caccgtaaac aggccgcttg agggaaataa gacgatgccg
    63721 ctttacccag tttaacctgc actttattct caacgacttg cctgtattgg ctccctttta
    63781 atcactttgc gtcgggaagt tatgctggaa aatttgaatc tctctctatt ctctcttatt
    63841 aacgcgacgc cagactcggc tccgtggatg atctcgttgg cgatttttat tgctaaagat
    63901 ttgattaccg tggtaccgct gctggccgcg gtgctttggt tgtgggggct tacagcacaa
    63961 cggcaactgg tgataaaaat cgctatcgcg ctggcggtca gcctgtttgt gtcctggacg
    64021 atgggacatc tttttccgca cgaccgaccc tttgtcgaaa atatcggcta taacttcctg
    64081 catcatgcgg cggatgactc attcccaagc gaccacggta cggtgatttt cacctttgca
    64141 ctggcatttt tatgctggca tcgcctgtgg tccggctcac ttttaatggt gctggccgtc
    64201 gtcattgcct ggtcgcgcgt ttatcttggc gtgcactggc cgctggatat gctcggtgga
    64261 ttgctggctg gtatgattgg ctgccttagc gctcagatta tctggcaagc gatggggcat
    64321 aaactctatc aacgtctgca atcgtggtat cgcttctgtt ttgcattacc gatccgcaaa
    64381 ggctgggtgc gtgactgatc tctgctaaaa agtgtagtat tgagcggctc gcttcaataa
    64441 ctattcagag ggattatgga aacacgtcgc gaagagcgca tcgggcagct gctgcaagaa
    64501 ttaaaacgca gcgataagtt acatcttaaa gacgccgccg ccctgcttgg ggtttcggag
    64561 atgacgattc gtcgcgatct gaacaaccac agtgcgcccg tcgttttgct cggcggctat
    64621 attgttctgg aaccgcgcag tgccagccat tacctgttaa gcgatcaaaa atcccgcctg
    64681 gtggaagaaa aacgccgggc ggcaaaaatg gctgcgacac tggtagaacc cgatcagact
    64741 ctcttttttg actgtggcac caccacgccg tggattattg aagcgattga taatgaaatc
    64801 ccttttaccg ccgtttgtta ttcgctaaat acctttctgg cgctgaaaga gaaaccccat
    64861 tgccgcgcat ttctgtgcgg tggtgaattt cacgccagca acgccatttt caaacccatc
    64921 gattttcagc aaacgttgaa taatttttgc ccggatatcg ctttttattc cgcggcgggc
    64981 gtgcatgtca gtaaaggcgc tacctgtttt aatcttgaag agttgccggt aaaacactgg
    65041 gccatgtcga tggcgcaaaa gcatgtgctg gttgtcgacc acagtaaatt tggcaaggtg
    65101 cgtccggcgc gcatgggtga cctgaaacgc tttgatattg tggtgagtga ttgttgcccg
    65161 gaagatgagt atgtgaagta cgcgcagacg caccgtatta agttgatgta ttaatgacgt
    65221 ataaccggat gacgtttcgc gccatccggt tatcagaaga ttaagagaac cagctgccga
    65281 accactgatg gaatttcatc atcacgaaat cccacatccg accaaagaat ccgccctctt
    65341 ccacattttc catcacgatc agcggacgct gctcaatgga tttaccgtta agctggaaat
    65401 caatggtccc gacaacctga ccttttttca gcggtgcggt aagctgcggt tccgttaacg
    65461 tgtagctcgc cttcaggttt ttcagctgcc cacgcggtat ggtcactgag cccgcttcgc
    65521 ctgccccaag attcacttcg ctcttatcac caaaccagac gcgctgagtc acaaaggtgg
    65581 catcaggttt aattggcgtc acggtttcaa agaagcggaa accccaggtc aataatttct
    65641 cagactcatt aaaacggata cggtcagttt tcgcccccag cactacggag attaaacgca
    65701 tatcgccctg ggtagccgaa gcaaccagat tatatcccgc gcctgccgtg gttcctgtct
    65761 tcatgccatc aacattcaga ttgctgctcc acagcagacg gttacggtta ggctgacgaa
    65821 ttttgttgaa ggtgaactct ttctctttat gaatggcgta ctcttccggc acatcgtgga
    65881 tcaatgcttt acccagcaat gccatatcgc gcgcggtact gaactgcccc ggcgcatcca
    65941 gaccgtgcac cgtctggaag gtagtgttgg tcagacccag ttttttggca taaccattca
    66001 tcaaaccaat gaatgactcc tggctcccgg caacgtaatc ggccagcgca atacaggcgt
    66061 cattaccgga ctggataatc acacctttgt tcaagtctgc caccgaaacc tgatcgcccg
    66121 gtttgaggaa cattaccgat gaaccacgca gtgccggatt tcccgtcgcc caggcatctt
    66181 taccgaccgt caccatatcg gtgagtttaa tcttatcggc cttaagcgcc tgcccaacca
    66241 catagctggt catgatttta gtcaggctcg cgggatccag tttctcatcc gcgttgcctt
    66301 ctgccagcac tttaccgctg gcgtaatcca ttaaaatcca tgcacgcgca tccacgctcg
    66361 gcgcttcaac ggtttgttcc gccgcgaatg ccgttggggc aaaaaggaat aaaaatgcag
    66421 aacccgctgc aagaccacga aggagagagg agtattgcgt cataaatgcc acccgagtat
    66481 ccattcaaaa aaaatacgtt atatcgccgt atgataagtt cggtgattaa taacgcacac
    66541 acaaacttaa agaaaccgta agttggtaaa gtttttaaag tttatgcaat atcctgccgc
    66601 ttcgctgctt ctggtgtcat ttttttgcca gctgttgctt aaaagttaac tttacttcac
    66661 catcggagtc ctgttcagcc tgtggctgcc tgtcggaaaa ggggtgagta tgattacgct
    66721 gtggggtcgg aataattcaa ccaacgtaaa aaaagtattg ctgacgctcg aagaactgga
    66781 actaccttat gagcaaattc tcgcgggccg tgagtttggg ataaatcacg atgctgattt
    66841 tctggcgatg aaccctaacg ggctggtgcc gttgttgcgt gacgacgaaa gtgatctcat
    66901 tctttgggaa tcaaacgcca ttgtccgcta tctggcggca cagtacgggc aaaaacgtct
    66961 gtggatcgac tcaccggcac gtcgtgcgga agcggaaaaa tggatggact gggcaaacca
    67021 gacgctcagt aatgctcatc gcgggatcct gatgggatta gtcagaacac caccggaaga
    67081 gcgcgatcag gccgccattg atgccagttg caaagagtgc gacgccctgt ttgccctgct
    67141 cgatgcggaa ctggcaaaag taaaatggtt ctctggcgac gagtttggtg tgggcgatat
    67201 cgctatcgca ccgttcatct acaatttgtt taacgttggc ctgacctgga caccgcgtcc
    67261 aaatctgcaa cgctggtatc agcaactcac tgaacgcccc gcagtgcgca aagtggtgat
    67321 gattcccgtt agctaattgc gtgggctaac tttaagtaat tccccactgg actcgtcggt
    67381 gagaacgtat aaataaccgt cgggtccagt gcgaacatca cgaattcgct gccctctgtc
    67441 cgttaaaata cggccatctt ctgtcacttt gtcgccgttg acgctcatca caatgacatc
    67501 tttatctttc agcgcgccaa taaataattt ttgctgccac tgggggaatt tatcgctgtt
    67561 atagaaggcc atgccgctca cagcgggcga atctttccag taaaaaacag gttgctcggt
    67621 cccggcgacg atctcccctt tcgcttccgg tatcttaaag cctgaatagt tgattcccca
    67681 ggttgccagc ggccagccgt agtttttgcc tttttgcggg atattaattt catcaccacc
    67741 gcgcgggcca tgttcattca gccacagtgc attactccac ggattcattg ccattccttg
    67801 cggattacga atgccataag accagatctc ggcgcgcaca ccggattcct ttataaaagg
    67861 attatcatcc gggatttcgc cctggtcggt cagacgcacc agtttgccct gtaatttatc
    67921 cagatcctgc gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa gataaccttt
    67981 accgtcgaat accagccgcc cgccaaaatg gttgccggta gacagttttg gcatctggcg
    68041 gaagacggtg cggaagtcgg tcacttttga gagatcatca cttaagcggc cataacccac
    68101 agcagttccg gctttgccat catcgccaac ttcggaataa cttaaccaga tgcggcgaga
    68161 ctgagcaaaa tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt gcgcccaaac
    68221 gtccggaact ccggaaagcg gcgcagataa tccttttcct gcttgccagt gacgcaactc
    68281 gccgccgcgc agagtgatta acataccgtg attatcgggt aaaaaggcca gtgcccaggg
    68341 atggtcgagt ttgtcttgca gtacttcgac atttaccgtt gcaggagccg cccagagagc
    68401 ggaagaaaga caaataaggg gcacaaggaa aaaggattgt cgatgcataa cgcctccgga
    68461 ttgactctgg cttaaagcgt agtcagtgga ggagataaca aattcatttt tacaaaaact
    68521 taaacatgaa gggggagacg ctttctcccc cttagttttc aggccttctc aagcatggcg
    68581 tgcttctgca ggctctggat actcagcgtt aagctcatca gacaattttc aagcttatcg
    68641 gcgttgacgg taataacagt cgggcaatca tggtgcccac tcatcaaaca tactgcggct
    68701 gtcgctaatg cttcttcagc atgatgaaga gcactccact cttcctgatc cagatgaaga
    68761 ttcaaccgca gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt atctcgtaga
    68821 tgattgcttt cgctgacgga catgtatcct tttgccttcc tcagttgcac gtatgcagcg
    68881 aggtcaacgc gtgcgtcgat caaccggttc agcagatcga ttcgctgtct gtcaacgaac
    68941 atacttcgat cctcctcttt ccagccttgt tgcaattaaa tagtatggct attttttgag
    69001 caaaatgctg tccaggatca attcttcacg ccgaattaac aaaatttaca tttccttcgc
    69061 ctccgccagc gaatgagatt gcgatacaaa ttttatccag aactctgtag aatccctgcc
    69121 ctgacctttc ctgtaactga atatttttta agctatgagc aaagtaactc cccagccgaa
    69181 aatcggcttt gtttcccttg gctgtccgaa aaaccttgtc gattcagagc gtattctcac
    69241 cgaactacgc actgaaggtt atgacgtggt accgagctat gacgatgcgg acatggtgat
    69301 cgtcaacacc tgcggcttta ttgacagcgc ggtacaagaa tcactggaag ccattggtga
    69361 agcgttgaat gaaaacggca aggtaattgt gaccggttgt ctgggggcaa aagaagatca
    69421 gatccgcgaa gtccacccga aagtgctgga aatcaccggg cctcatagct atgagcaggt
    69481 tctggagcac gttcatcact acgtgccaaa accgaaacac aacccattcc tgagcctggt
    69541 gccagaacaa ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa tttcggaagg
    69601 ctgtaatcac cgctgcacct tctgcattat tccgtctatg cgcggcgacc tggtgagccg
    69661 tccgattggc gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg ttaaagagat
    69721 tctggtgatc tcgcaggata cttctgccta tggcgttgat gttaaacatc gtactggctt
    69781 ccacaacggc gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt tatcgaaact
    69841 ggggatctgg acacgtctgc actacgttta cccttatccg catgtggacg acgtcatccc
    69901 actgatggca gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc acgccagccc
    69961 gcgcattctc aaactgatga agcgtccggg ttctgtagat cgccaactgg cgcgcatcaa
    70021 acagtggcgc gaaatctgcc cggaactgac cctacgctca acctttattg tcggcttccc
    70081 tggcgagaca gaagaagatt tccagatgct actcgacttc ctgaaagaag cgcgtctgga
    70141 tcgcgttggc tgctttaaat acagcccggt tgaaggtgca gacgccaatg ccctgcctga
    70201 ccaggttccg gaagaagtta aagaagaacg ctggaaccgt ttcatgcagc tgcagcagca
    70261 gatttccgcc gagcgcctgc aagagaaagt gggccgtgaa attctggtga ttatcgacga
    70321 agtggacgaa gaaggcgcga ttggtcgcag catggcagat gcaccggaaa tcgacggcgc
    70381 ggtttatctc aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg tgaaagtcga
    70441 gcacgccgat gagtacgatt tgtggggtag ccgggtttaa ttaattcagg ccgggcaaaa
    70501 tcatttgtcc ggcctgcgaa taatgattac tgccagctaa tctgagccct gccgtttttc
    70561 ttactcttat acagccgctc atccgccagc attttcaagc gttcgacggt ggtacattca
    70621 tttgaatgcg ctacaccgat actggtactt aaattaatgc ttcccacctg gaaatgaacg
    70681 atttctttta gaatcgatag caactcttcc agcgttccac ccgtataaat caccgcaaat
    70741 tcgtcaccgc cgaagcggta gattttacct ttatctaccg ggatctgtga tttaaggatt
    70801 ttcaccactt tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc gttaatgttt
    70861 ttgaatttat cgacgtcaaa caggcacaaa taaccgcctt tttccggcag cgcttttaaa
    70921 tcttcgtcaa aggattttct gcccattgct ttggtcaggt cgtccaggaa cgccgagtta
    70981 tacatactgt agagtttttt gacatcggca gccgaaaaga aaagcccgcc atccagtacg
    71041 cgttcaaaat ggttattaat gatggcttct ttctccacaa taccacgatt aatcactctg
    71101 cgtaccagat agaggcaact ggtacccgat atcactgcca ccaatgcggc aaagaaaata
    71161 atcaatgtac agacccgcaa ataaacatct tttttatacg caaatacgta caatccggtc
    71221 ggcaaatagg cgaagtgctc catcgcttcc gaaacggtga agatggcgtt tttagcataa
    71281 tattcatcac cggtcaccgg tttaccgttc ttaaatagag agaacatcag gttctcgctg
    71341 tccatcgcca gcgattttga cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga
    71401 ctacgattgt gagttaccgg atagaggtaa tagacgaagg ttttaccgtc aaaagagata
    71461 aaccctgtat ttttgacatg tcgatacttc acaacctgca taatatctgc acaataattt
    71521 ttctcgaact cctttacctg aatacattcg tcactggaaa tcagataagg accgacatcg
    71581 tacaaataag agggggaaac cgagtcggcg taagctgtgc gcaaaccaac caacagctgt
    71641 tcctccgtgg gatttagcgt gttgataaga tcaattgcca cgctacgccg atattcagca
    71701 atatttgcca ccgcaaaagc agtatcaata acggactgtt tcaggctata cacccgttcc
    71761 tttaccattt gagtgtagat cccgcaggca actgctgaaa tcatgataaa aatcagcaga
    71821 ctgactgtaa gtacgaactt attgattctg gacatacgta aactactctt ttactaattt
    71881 tccactttta tcccaggcgg agaatggcat gggtttctgg tacagatacc cctgagcctg
    71941 aacacatcct aatttaatta attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat
    72001 caccgggatc cggttatagc gtgccagatc gaaaataatg cgcacaattt ttcccgcagt
    72061 accgaaaata tcgccggtaa attgcgagtc cacctttatg ctgtctggat taatttcata
    72121 aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca atcgatatcc gtaccccttg
    72181 tttacgcagt tgttcaatat tgagataaaa atgttcatta attcgctcga tcacggtttc
    72241 agtgatctca acacagaggc tgttcgcctt caggccattt tctttcatga tggtggtaaa
    72301 acgctcatga aagtgcggtt cgtttaactg ccagggcgaa acattaatat gcagtaagaa
    72361 atcatccttc agcgacgcac gctgactaaa atgtctgaat tcacccattg ccgttttaat
    72421 aatctgataa cccagctcat tgataaaccc gatatcttca gcaatggtaa tgaacttcag
    72481 cggtgaaatg atcccccgct ccgcagaaac ccaacggcac aacgcctccg cgcccagaat
    72541 ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg aatagttcgc caatttccat
    72601 tgcatggcga atattacgcg caatccagat attctcttct atttcgttga gtttcgcgtc
    72661 atcacagaaa ataaactcgg agaacatatg gtcacggata ctggaaatcg ccagtttcag
    72721 tttttctgcg taactccagg cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg
    72781 gatgcgcaga ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg ctattttacg
    72841 ggtaaatgtc tgtacatttt cccgacacag aagtaaatag agatcggcat tgaatgtcac
    72901 gatatcgcag cattgcgcat atttctcggc aattcgctga cacaatttcc tgacgatttt
    72961 attgcgttca tcaacaccaa acaccgcttc cacggcttcc agaccacaca gtttgatgat
    73021 ccccacagcc agatactgat tattgttgga ctcaataatc gccatatttt taataaaacc
    73081 gctttcctga tcgtaagaga tcagtgagag caatttattt gaccgttcaa tttcggcatc
    73141 tttcatttcc gcgatcgaat ttttgatgct gataatctct ttcgcatcgc cttcgcgaat
    73201 tttcagagcc tcgatctgat ccgttgagtc gggcaaaaac gccagcgtat taaccagctc
    73261 ggtcatttca gaaaattgcc gacggaacac acgagtggtc atgaaatata ccaccagcaa
    73321 cacgataata atgaacgcaa acggtagata aaaaagttta tctgatgcgt tcaataaatt
    73381 accgccagga taataaaaat agatttcgaa caatgcatta tttggcactg ctgatgtctt
    73441 tgcaatatac tccgtatcga cagaatcaat aatattataa ttaccttcgc tattataaat
    73501 aatcagtttt tcacgagcga gttgattctt ggcaatgatc tcatgacctt gtttcaaaaa
    73561 tagaacttca tcattagcgc tgatttgatc aagatactcg ttcagatacc gccagtttaa
    73621 atcgacatga attttatcta ccgctaacaa atcgtgatct ttgccgttga tacgtagcgt
    73681 aaaagagata cattttttac ccggacgatc aaagcaattg tatgccggat tccagctgtt
    73741 gttagagtca tctgaagcca ccgaccacgg tcgtttcgat gggttaaaat cggcaaaaat
    73801 aacccgccct gactcgtcga caagcggtcc atttacttgt tcctgatgat aaacaacgat
    73861 tttatcattc gccctccgtg aaaagagata atatttatta tcatcaagta ccagtcccac
    73921 cgagtctaaa aatggcatca tttccatttt cagaatcacg gcgcgttgaa tctcctctgt
    73981 attttttaat aaatacagtg agtcctttaa tgtcagggcc gaacgttcaa tatctttaaa
    74041 gaaatagcca acgtttttct gaataagttc aaagcggcta tcggcacgtt ttaccgcgcg
    74101 ttccaatacc agttgatagt taatgatgaa aaaaagacca ataaatgaca gtgctgccaa
    74161 taaaaataaa attatcagcc ttatctttat cttttcgtat aaactcagca tgttaataca
    74221 tttaacaaaa taactatctg aacgctgtgc cgctgcaaaa atagtccctt gctgcggctt
    74281 tttaaccaaa ttacccgatt ctacaaaatc agcagaaagt tacaacactt gcttagggca
    74341 ataatttcct gtgaggtgat taccctttca agcaatattc aaacgtaact atcctttaat
    74401 tttcggatcc agcgcatcgc gtaaaccatc gcccaacaaa ttgaacgcca gtacggtcag
    74461 aaaaatagcc agggccggaa aaacagcgac atgcggcgcg ataaccatat ccgctcgtgc
    74521 ctcattgagc attgctcccc actctggtgt cggcggctgc gcaccgaggc cgagaaatga
    74581 caggctggcg gcagagataa tcgaggtgcc aatgcgcatg gtgaaaaaca ccacgataga
    74641 cgagacggtc ccaggcaaga tatgacgcaa taaaatggtc atatcgctgg caccaatact
    74701 gcgtgctgac tcaataaagg tttgctgttt caacaccagc gtgttgccgc gcaccagtcg
    74761 ggcaaacgcg gggatggaaa aaatggcgac tgcgataatc acgttagcaa tgccgcttcc
    74821 caacaccgca acaacagcga tcgccagtaa aatacccggg aaggcaaaca gcacatcgca
    74881 aatgcgcatg atcaaccgat cccaccagcc ttcataatat ccagccaaca agcccagcaa
    74941 cgtcccgatc gccgcaccga taaacacggc aaacacgccc gccgccagcg agatttgcgc
    75001 accaaccagg acacggctga aaatgtcgcg ccccagtgaa tcgacgccaa accagtgctg
    75061 caaagaaggt ccgttattca gattgtcata atcaaaataa ttttcggcgt catagggagc
    75121 gatccagcgt gcaaaaatgg ccaccacaat caataaaata acgaataatg cggcggtcat
    75181 cgccatatgc tgacggcgaa atcgtcgcca gaattcatgc cacggtgtac gcacctggtc
    75241 aggtttgacc agtggcatgg cgtttaacac cgcctgacgt cgccagttaa atagtcgcat
    75301 ccttacttgt acctgatagc cgggttaatg gcggcgtaaa gcacatccac cactaagttg
    75361 ataagaataa attccagcga gaaaagcaga atttccgcct gaatcaccgg gtaatcgcgc
    75421 atttctacgg agtcaacgag taagcgtcca agtccgggcc agttgaaaac tttctccacc
    75481 acgatagaac caccgagcaa aaagccaaac tgtaagccca tcatggtcac taccgggatc
    75541 atcgcgttac gtagcccgtg tttgaggaca acccaggttt cgctcacccc tttcgccctc
    75601 gcggtacgca tataatcttc gcttaaaaca tcgacaaacg acgcgcgggt aaagcgcgcc
    75661 atcacggctg ccactgccgc gccgagcgtc agggagggta aaatgtagtg ctgccaactg
    75721 tctgctccca cggtaggcag ccagcccagt tcaacggaga atacctgaat taaaagcatc
    75781 cccagcgcaa atgccggaaa cgagatcccc gacaccgcaa tggtcatact caatcgatcc
    75841 ggccaacgat tacgccagac ggcggcgata attcccgccg ccataccaaa tatcaccgcc
    75901 cagaccatac tggttatggt cagccacagc gttggcataa agcggctggc aatctcatcg
    75961 gcaaccggac gacgcgacac catcgacagg ccaaaatccc cctgcacagc attgctgata
    76021 tagtgccaga actggtgata cagcggctga tccaacccca gctgctgacg caccagttct
    76081 ataacctgcg catcagcttc gggcccggca atcaatcgcg ccggatcgcc gggcagcata
    76141 tgcacaaata aaaacaccag caccgagacg ataaacagcg tcggaatcaa ccccagtaag
    76201 cgtttgataa cgtaattaag cattccactc cctgcgttgc ttattgcaaa tccgcgtctt
    76261 caaagctgaa gccggtgtct ggcatgatcc aaaaaccggt caggttttta ctgtgtgccg
    76321 acaccagttt ttctaccacc agcgggatcc acggcgactc ttgccagatg atatcctgcg
    76381 ccgccttata caagcgggtc ttttccgccg gatcattagt tttcagtgcc tgagccagga
    76441 agtcgtccac ctgtttattg ctgtaaaatg cggtattaaa cagcgtcggt ggccagtttt
    76501 gcgaggcgaa cagtggcgat agcgcccagt ccgcttcgcc ggttgaagcc gaccagccag
    76561 tgtagaacat ccgcacgccg ctctcttttt gccctttacc ttcaacttct gccgcccgct
    76621 gtccggcatc catcgcggtc acctgggctt taatcccgac ctgcgctaac tgctgctggg
    76681 taaattgcag cactttctgc gcggtgctgt ggttatgtga cgaccacagc gtggtactga
    76741 aaccgttggg atatcccgcc tctttcagta attcgcgcgc tttcactgga tcgtaaggcc
    76801 acggtttata actttgcgcg taggcgatac ttggcggtac cacaccagta gctggcgttg
    76861 catagcccgc aaaggcaact ttcaccagcg ccggacggtt aatggcgtaa ttcagcgcct
    76921 cacggacctt cgggttatcg aacggctttt gcgtcacgtt catactgata taacgctgca
    76981 taattgacgg actggccatc aactcgatat ttttgttttt ctccagcagt gtggcctgct
    77041 cgtagggaat ggggaaagca aactgcgctt caccggtttg cagcattgcc gcgcgggtgt
    77101 tgttatccgc caccggacgc caggttatgc tgtccagttt gggcaatcct ggctgccagt
    77161 aacccgcgaa ttttttcacc ttcacaaaat cggtctgatt ccaggtatcc agttcatacg
    77221 gtccggttcc caccggatga aaaccaatct ccttgccata tttttccagc gctgccggtg
    77281 aaatcatcgc ggtcgccgga tgggcaagaa tattaataaa cgctgagaac ggctgtttga
    77341 gggtaatctt taccgttgtc ggatcgatcg cctccgtttt ggcaatattc ttatacaggt
    77401 tatagcgttt aagatgattc gccggatcgc tggcccggtc cagattcgct tttaccgcca
    77461 ctgcgttgaa atcggtgcca tcctggaatt taattccttc ccgcaatttc acgatgtaag
    77521 taatgccgtc atcggaaacg gtataactct ccgccagcac gtttttcagt ttcatctctt
    77581 tatccagacc gaacagcccc tggtaaaacg atttcgctac ggcctgagat aacgtgtcat
    77641 ttgcgtcata cggatcgagc gtggtgaaat tcgatcctac cgccaccacc acatctttgg
    77701 cagcgaatgc acaagatgcc atcaacgctg tcgcaatgcc cagcgccact aacccactac
    77761 ggtgtacagc tcttgccatt ttattctccg cctgaatgtt atctacgcat gaatgcgtat
    77821 tctgattgtg gttgtgcgac gtaatgcccc ggcccgacgc attgcaacga gacggctgcc
    77881 acctcttcgc cacgcagatg aatattgctg ggaagatcgt ccgacagcag tacacgctgc
    77941 ggtcgttgtc gggacggttc agcgaccgga actgccgcca gtaatttacg cgtataagga
    78001 tgctgcgggt tttcgaagac cgcgcgccgt ggaccaattt caacaatttg cccgagatac
    78061 atcaccgcca cacgatgact aatccgctct accacggcca tatcgtggga gataaacaga
    78121 tacgcaatgc cgaaatcacg ctggagatcg agcaacaagt tgataatctg cccgcgaata
    78181 gaaacatcca gcgccgaaac ggcttcgtcg gcaatgatca cttttggatt caatgccaac
    78241 gcgcgagcaa tgcagatgcg ctggcgctga ccgccggaaa actcatgcgg gtaacgccag
    78301 gcatgttcag gtaacaggcc cacgcgctcc agcaaccacg caacgcgtgc agccgcgtct
    78361 ttacctggca ataaaccgtg tacacgcagc ggttcgataa tcgaatcacc gatggtctga
    78421 cgtgggtcca gcgaagcgta agggtcctga aaaataaact gaatatcccg gcgcaatgcc
    78481 tgaagtttgc cgggtgacaa ggtatcgatt ctctgaccgt taaagataat ttcgccgccc
    78541 tgcgattcga ccaggcgcag caacgcccgc ccggtagtgg atttaccgct gccagactcg
    78601 cccaccagcg atagcgtttc gccaggccag agatcaaaac taactttctc aacggcatgc
    78661 acttcccgcg ttacgcgatt caacaaaccg ctgcgcaaag ggaaacgggt gacaagatta
    78721 cgcactcgta aaacaggttc gccatccacc accgttttct gctcgatggg gggggcctgt
    78781 ttcgctggat gttcaagcga tatcaacggg aaacgtcggg gataatctaa ccctttcatc
    78841 gcaccaagtt gcggaacagc agctaacagc gcacgggtgt aaggatgttg cggtgcatga
    78901 aaaatctgtt cgacggtacc cgtttccacc gcctcgccct gatacatcac cagtacccga
    78961 tcggcaatct ctgccaccac gcccatatcg tgagtgataa agataacgcc catcgacatc
    79021 tctttttgca atactttgat taattgcagg atctgcgcct gaatagtgac atccagcgcg
    79081 gtggttggct catcggcaat cagcaccgcc gggcggcatg acagcgccat cgcaatcatc
    79141 actcgctggc gcatcccgcc agagagttga tgcggataac gtgaaagaat ggtttgtgcc
    79201 tcaggaatgc gtacctgatc cagcatccgc ttcgcctcga ccatcgcttc ttcacgactg
    79261 gcgttctgat gcagacgaat tgattcggca atctgttcac ccacagtaaa taccgggttc
    79321 agcgatgtca tcggctcctg aaatatcatc gccatatccg caccgcgaac atggcgcatt
    79381 tgtgcagcgc tctgctcgct aagttcaatc acttcgcgac tgcgccgctg caacagcatt
    79441 ttatcgcact gtactaaacc gcccgcctgt tccaacaggc gcatcaatgc caacgcagtc
    79501 actgacttac cggagccgga ttcgccaaca attgccagcg tctcaccgcg ttgcagacta
    79561 aaagagagat tgcggaccgc agctattttc tgctggtcct gcataaaggc aatattcaga
    79621 ttttcaaccg ccagcacatt accggcatca agttcatcac tgtgtggcaa cggtgtcccc
    79681 tttttcacgg tagataccgg tggttggcgt atcgcctgcg tagccccagg cgcgatacat
    79741 tccttcggtg ttaaacggta gcgcgacatt cccttcatgg tcgatagcga ttaagccacc
    79801 gctaccgcca agcgcaggga gtttttccat tactacccgc tcgcaggctt ccgcgagact
    79861 taatccgccg taatccatta acgcggcgat gtcatatgcc gccagcgcgc ggatgaagac
    79921 ttcgcccgtg ccggtacaag aaaccgccac actggcgtta ttggcgtagc atccggcacc
    79981 cactaaggga ctatcgccaa ctcgtccggg taatttattg gtcattccgc ctgtggacgt
    80041 ggctgccgcc aaattgccgt ctaaatccaa cgccacggcc cccacggtgc ccattttctg
    80101 tttttcatcc agtggcgcac cgctatggtc gaggacggtt gccccttcct tgcgcgctgc
    80161 cagtagttgt tcataacgca aaggcgtgga gaaaatctcc ggcgagacgc gctccatgcc
    80221 acgagcaaac gcaaaatttt ctgccccttc gccaatcatc atcacatgcg ggctttgctc
    80281 catcaccagc cgggcggcaa gaaccggatt acgcagatga ctaacgcccg ccaccgcacc
    80341 ggctttcagg gtgttaccat ccatcacaca ggcgtccagt tcatgggttt catcacgcgt
    80401 aaagacagcg ccaattccgg cgttaaacag tggacactct tccagcagac gcaccgcttc
    80461 cgtcaccaca tccagcgcac tttcgcccgc ttccaacatt ttctgcccgg tttcaacaat
    80521 ggcagacaac gcctcaatgt agcgtaattc ctgttgcaga ctcatctgcg cgcggctaat
    80581 tgcacctgcg ccaccatgaa ttgcaatgac tgctttgccc atagtattcg tccattaagg
    80641 gtggctcgcc acttttgcta taaatatcag aatttttatg aattactcat tcaatttttg
    80701 aatatagaaa gatggatatg gcatgtaaag gcaggagcca acgctacgcc cgaggtggca
    80761 tacagtctgt ggcgtttttc tgacataata ggcaaattcg attttgcctc cgcaggagtg
    80821 ttttcatgga atttaccacc ggattgatgt cgctcgacac cgcgcttaat gagatgcttt
    80881 ctcgcgtcac cccactgacc gcccaggaaa cgctgccact ggtacagtgt tttggtcgta
    80941 ttctggcgag cgatgtcgtt tcgccgcttg atgttccggg gtttgataac tccgcaatgg
    81001 acggctacgc ggtgcgttta gccgatattg cctccaggca accgctgccc gttgccggta
    81061 aatcctttgc cggtcagccg tatcatggtg aatggcctgc gggcacctgc attcgtatta
    81121 tgaccggtgc gccggtgccg gaaggctgcg aagcggtggt gatgcaggag cagactgaac
    81181 aaacggacaa tggcgtgcgt tttactgctg aagtgcgtag cgggcaaaat attcgccgtc
    81241 gcggtgaaga tatctctgca ggtgcggttg ttttcccggc gggaactcgc ctgactaccg
    81301 cagagctgcc agtaattgct tcactgggga ttgccgaagt tccggtgatt cgtaaagtgc
    81361 gtgtagcgct tttttctacc ggtgatgaac tccagttgcc cggtcagccg ctgggcgacg
    81421 gccaaatcta cgacaccaac cgtctcgccg tacacctgat gttagaacag ctgggttgcg
    81481 aggtaatcaa cttagggatt atccgcgacg atccccatgc cctgcgcgcc gcatttattg
    81541 aagccgacag ccaggcggat gtagtgatca gttccggcgg tgtttcagtg ggtgaagcgg
    81601 attacaccaa aacgattctt gaagagctgg gggagatcgc cttctggaag ctggcgatta
    81661 aaccaggtaa accgttcgcg ttcggtaaac tcagcaatag ctggttctgc ggcctgccgg
    81721 gcaacccggt ttcagcgacg ctgaccttct atcaactggt acagcctttg ctggcaaaac
    81781 taagcggcaa caccgccagc ggcctgcccg cgcgccagcg cgtacgcaca gcgtcccgcc
    81841 tgaaaaaaac gccaggacgt cttgatttcc agcgcggcgt gctgcaacgc aacgccgacg
    81901 gtgaacttga agtgacgacc accggacatc agggttcaca tatatttagc tcctttagtc
    81961 tcggcaactg ctttatcgtg ctggaacgcg atcgcggcaa tgtggaagtg ggcgaatggg
    82021 tggaagtaga accgtttaac gcgttgttcg gaggcctgta atgacggaac tcagcgatca
    82081 ggagatgctg cgctacaacc ggcaaatcat tctgcgcggt tttgattttg acggtcagga
    82141 ggcgctaaaa gattctcgtg tgctggtagt cggcctcggt ggactcggct gcgcagcttc
    82201 gcagtatctg gcaagcgccg gtgtcggcaa cctgacgctg ctcgacttcg actcggtttc
    82261 gctctcgaat ctgcaacgcc agacgctgca cagtgatgct acggtcgggc aaccgaaggt
    82321 agaatccgcc cgtgacgcca tgacgcggat caacccacat atcgcgatta cgccagtcaa
    82381 tgcactgctt gatgacgctg aacttgctgc gatgattgca aaacacgatc tggtactcga
    82441 ctgtacggat aacgttgcgg tgcgtaatca actgaacgct ggctgttttg ccgcgaaggt
    82501 gccgctggtt tccggcgcgg caattcgtat ggaagggcaa atcacggtct tcacttatca
    82561 ggacggcgaa ccgtgctatc gctgccttag ccgtttgttt ggcgagaacg cattaacctg
    82621 cgtagaagca ggcgtgatgg caccgttgat cggcgtaatt ggttcgttgc aggcaatgga
    82681 agcgatcaaa ctgctggcag gttatggcaa accggccagc gggaaaatcg tcatgtacga
    82741 tgcaatgacc tgtcagtttc gcgaaatgaa actgatgcgc aatccggggt gtgaggtgtg
    82801 cgggcagtaa ttgccacctc caggccagat aagacgcggc actccgccac gtcatcagca
    82861 atttatgagg atgcagaacg tccggttaaa tcgacgttct gccaaacgct cccagccagt
    82921 cctgttcaaa cttcgccaca gcggcttcca ccgccggata gctaatcatc tgttgtgcca
    82981 catccagtgg cagcgtaatt gattcacatc ctgccagtaa gcagtccagc gcctgacgcg
    83041 gggttttgaa actcgctgcc agcactttcg cctgcggcgc atgcattttc aataactggt
    83101 gtaagtcggt cacagtctga atgccgctac cgccctgagc atcaatacga ttaacgtaag
    83161 gcgcaacata ttccgcacct gccagcgccg acagcagccc ttgtgctgcg ccatataccg
    83221 cggttcccag cgttggaatc ccttccgctt ttaacatctt aatagctgcc agcccctcgg
    83281 cggtcaccgg aactttcacc acgatatccg caataataga acgcaattta cgtgcgtcat
    83341 tcaccatccc ttcagcagtg gtagccatta cctgggcaaa cagacgcccc tgaccgccca
    83401 tcgcttcatg aagttgcgga agcacaacat ccagcggttt gttacccgcg gcgataatgc
    83461 ttgggttagt ggtcacaccc gccagcggaa aaatacgtga cagcgccttc accgcaacaa
    83521 cgtctgaagt atccagatac agttccataa ccatcctcaa aatgtacttt ttgtctgaat
    83581 gtagactatc acgggaaaac tctctgaaca gttgacgcac atcaatctaa ctttcattcg
    83641 aaagcatttt aatgtttgaa cgaaacagag aggcaactat gattttcaat attcagcgct
    83701 actcgaccca tgatggcccc ggtatccgca cggtcgtatt tcttaaaggc tgttcgctgg
    83761 gctgccgctg gtgtcagaac ccggaaagcc gcgcccgcac gcaggatctg ctgtatgacg
    83821 cacgactgtg tctggaaggc tgcgagctgt gcgctaaggc cgcgccggaa gtgattgagc
    83881 gcgcgctgaa tggtttgctt attcatcggg aaaagttaac cccggagcat ctgacggcgt
    83941 taaccgactg ctgtccgaca caggcattaa ccgtgtgtgg tgaagtgaaa agcgttgagg
    84001 agatcatgac gaccgttctg cgcgataaac cgttttatga tcgcagcggc ggcggtttaa
    84061 cgctttcggg tggtgagccc tttatgcagc cggaaatggc gatggcgcta ctgcaagcca
    84121 gccacgaggc aggcattcat actgcggtag aaacctgtct gcatgtgccg tggaaatata
    84181 tcgccccttc tctgccctat atcgatctgt ttcttgccga tttaaaacac gttgccgacg
    84241 cgccgtttaa acagtggacc gacggtaacg ccgccagagt gctggataac ctgaaaaaac
    84301 tcgccgcagc gggcaaaaaa atcattatcc gcgtgccgct gattcagggc tttaatgccg
    84361 acgaaacctc tgtaaaagcc attaccgatt ttgccgccga cgagctgcac gttggcgaaa
    84421 ttcattttct gccctaccac acgctgggca tcaacaaata tcacttactt aatctgccct
    84481 atgacgcccc ggaaaaaccg cttgatgcgc cagaactgct cgactttgcc cagcagtatg
    84541 cctgccagaa agggttaacc gcgaccttac gaggataaca accatgacca cactgaaact
    84601 ggacacgctc agcgaccgca ttaaagcgca caaaaatgcg ctggtgcata ttgtgaaacc
    84661 gccagtctgt accgagcgcg cgcagcacta taccgagatg tatcaacaac atctcgataa
    84721 gccgatcccg gtacgtcgcg cgctggcact ggcgcatcac ctggcgaatc gcaccatctg
    84781 gatcaaacac gatgagttga tcattggcaa ccaggcaagc gaagttcgcg ccgcgccgat
    84841 cttcccggaa tatactgtct cgtggatcga aaaagagatt gatgatctgg cagatcgtcc
    84901 cggtgctggc tttgcggtga gcgaagagaa caaacgcgtt ctgcatgaag tgtgcccgtg
    84961 gtggcgcggt cagaccgtac aggatcgctg ctacggcatg tttaccgatg agcaaaaagg
    85021 tctgctggcg accggaatca ttaaagcgga aggcaatatg acctccggcg atgcgcacct
    85081 ggcggtgaat ttcccgctgc tgctggaaaa aggtcttgat ggtctgcgtg agaaagtagc
    85141 ggaacgtcgc tcgcgcatca atctgacggt gctggaagat ttacatggcg aacaattcct
    85201 gaaagcgatt gatatcgtgc tggtggcagt cagtgaacac attgaacgtt tcgctgccct
    85261 ggcgcgtgaa atggctgcta ccgaaactcg cgaaagccgt cgcgatgaac tgctgacgat
    85321 ggcagaaaac tgcgatctta tcgcccacca gccgccgcag actttctggc aggcgctgca
    85381 actgtgttac ttcatccaat tgattttgca gatcgaatct aacggtcact cggtatcgtt
    85441 tggtcgtatg gaccagtatc tctacccgta ctatcgccgc gacgttgaac tcaaccagac
    85501 gctggatcgc gaacacgcca tcgagatgct gcatagctgc tggctgaaac tgctggaagt
    85561 gaacaagatc cgctccggct cacactcaaa agcctctgcg ggaagtccgc tgtatcagaa
    85621 cgttaccatt ggcggacaaa atctggttga tggtcaacca atggatgcag tgaatcccct
    85681 ctcttacgcg atcctcgaat cctgcggtcg cctgcgttcc actcagccta acctcagcgt
    85741 gcgttaccat gcaggaatga gtaatgattt cctcgacgcc tgcgtacagg tgatccgttg
    85801 cggcttcggg atgccggcgt tcaacaacga cgaaatcgtg atcccggaat ttattaaact
    85861 cggtattgaa ccgcaggacg cttacgacta cgcagcgatt ggttgtatcg aaaccgccgt
    85921 cggtggcaaa tggggctatc gctgtaccgg catgagcttt atcaacttcg cccgcgtgat
    85981 gctggcggcg ctggaaggcg ggcgtgatgc caccagcggc aaagtgttcc tgccacaaga
    86041 aaaagcgttg tcggcaggca actttaacaa tttcgatgaa gtaatggacg cgtgggatac
    86101 gcaaatccgt tattacacgc gtaaatcaat cgaaatcgaa tatgtcgtcg acaccatgct
    86161 ggaagagaac gtgcacgata ttctctgctc ggcgctggtg gatgactgta ttgagcgagc
    86221 gaaaagtatc aagcaaggcg gcgcgaaata tgactgggtt tctggcctgc aggttggcat
    86281 tgccaacctc ggcaacagcc tggcggcagt gaagaaactg gtatttgaac agggcgcgat
    86341 tggtcagcaa cagctggctg ccgcactggc ggatgacttc gatggcctga ctcatgagca
    86401 actgcgtcag cggctgatta acggtgcgcc gaagtacggc aacgacgatg atactgtcga
    86461 tacgctgctg gctcgcgctt atcagaccta tatcgacgaa ctgaaacagt accataatcc
    86521 gcgctacggt cgtggtccgg ttggcggcaa ctattacgcg ggtacgtcat ctatctccgc
    86581 taacgtaccg tttggcgcgc aaactatggc aacgccggac gggcgtaaag cccacacccc
    86641 gctggcagaa ggcgctagtc cggcctctgg tactgaccat ctcggcccta ctgcggtcat
    86701 tggctcggtg ggtaaactgc ctacggcagc gattctcggc ggcgtgttgc tcaaccagaa
    86761 actgaatccg gcaacgctgg agaacgaatc tgacaagcag aagctgatga tcctgctgcg
    86821 caccttcttc gaggtgcata aaggctggca tattcagtac aacatcgttt cccgcgaaac
    86881 gctgctggag gcgaaaaaac atccggatca gtaccgcgat ctggtagtgc gtgtcgcggg
    86941 ttattccgcc ttcttcaccg cgctctctcc agacgctcag gacgatatca tcgctcgtac
    87001 tgaacatatg ctgtaatctc ttcggcccgg ctgcggcgtc gccgggctaa atcattcaca
    87061 tcatcaattt catccttact ttcattcgaa atataatttg tgctctgcgt cacattgtta
    87121 ttacactgtt acggtttcat tttcgagcca ggagcacacc tatgagcgta aaagttatcg
    87181 tcacagacat ggacggtact tttcttaacg acgccaaaac gtacaaccaa ccacgtttta
    87241 tggcgcaata tcaggaactg aaaaagcgcg gcattgagtt cgttgtcgcc agcggtaatc
    87301 agtattacca gcttatttcg ttctttcctg agctaaagga tgagatctct tttgtcgcgg
    87361 aaaacggcgc actggtttac gaacatggca agcaactgtt ccacggcgaa ctgacccgac
    87421 atgaatcgcg gattgttatt ggcgagttgc taaaagataa gcaactcaat tttgtcgcct
    87481 gcggtctgca aagtgcatat gtcagcgaaa atgcccccga agcatttgtc gcactgatgg
    87541 caaaacacta ccatcgcctg aaagctgtaa aagattatca ggagattgac gacgtactgt
    87601 tcaagttttc gctcaacctg ccagatgaac aaatcccgtt agtgatcgac aaactgcaca
    87661 tagcgctcga tggcattatg aaacccgtca ccagtggttt tggctttatc gacctgatta
    87721 ttcccggtct acataaagca aacggtattt cgcggttact gaaacgctgg gatctgtcac
    87781 cgcaaaatgt ggtagcgatt ggcgacagcg gtaacgatgc ggagatgctg aaaatggcgc
    87841 gttattcctt tgcgatgggc aatgctgcgg aaaacattaa acaaatcgcc cgttacgcta
    87901 ccgatgataa taatcatgaa ggcgcgctga atgtgattca ggcggtgctg gataacacat
    87961 ccccttttaa cagctgacct ctctaccgga gccgtgcgct ccggttttcc cttcttttta
    88021 catcctgtac atcacatcaa atcattaaac ttgcattaac ttatttataa cttatattgt
    88081 cacatgcaaa tcagaatggt ttcgaatgaa agttaatgag aggtggttat ggcttctact
    88141 tttaccagcg acacattgcc tgccgatcac aaagcagcta tccgtcagat gaagcacgcg
    88201 ctgcgggcgc agcttggcga cgtccagcag atctttaatc agctaagcga tgacattgcc
    88261 acgcgagtgg ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg gccggtgctg
    88321 tcttatgccg atatcaaagc aggtcatgtt actgcagagc agcgcgaaca gattaaacgt
    88381 cgcggttgtg cggtgataaa aggccatttc ccccgcgaac aagcgctagg ctgggatcag
    88441 tcgatgctgg actatctgga ccgcaaccgc tttgacgagg tctacaaagg ccccggcgat
    88501 aatttcttcg ggacgctcag cgcttcacgt cccgagattt accccatcta ctggtcgcag
    88561 gcgcaaatgc aggcccgcca gagtgaagaa atggcgaatg cgcagtcgtt tctcaatcgt
    88621 ctgtggacat ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag cgtcatctac
    88681 cctgaccgta tccgccgccg tccgcccgga acgacctcca aaggtcttgg agcgcatacc
    88741 gactccgggg cgctggaacg ctggctgctt ccagcgtatc agcgcgtttt cgccaacgtc
    88801 tttaatggca atctggcgca atatgatccc tggcatgcgg cacatcgtac ggaagttgaa
    88861 gagtacacgg tggacaacac caccaaatgt tccgtgtttc ggacattcca gggctggaca
    88921 gcgctctctg atatgctgcc tggtcagggg ctgctgcacg ttgtgcccat tcctgaagcc
    88981 atggcgtacg tactgttacg tccgctgctt gatgatgtgc cggaggatga actgtgcggc
    89041 gtagcgcccg gaagagtgtt gccggtatca gagcaatggc atccactgtt aattgaggcg
    89101 ttaaccagca ttccaaaact cgaggccgga gactccgtct ggtggcactg cgacgtcatc
    89161 cattccgttg cccccgttga aaatcaacag ggttggggca acgtgatgta cattcctgcg
    89221 gcaccgatgt gcgagaaaaa tcttgcctac gcgcacaagg tgaaggccgc actggaaaaa
    89281 ggcgcatcgc cgggcgactt cccgcgcgag gactatgaaa caaactggga aggacgcttt
    89341 acgcttgccg acctcaacat tcacggtaag cgagcgttgg gcatggatgt ttgagagtta
    89401 atggaagtga attgatgtgg gattaagaaa atcgattcga cgttactcag attgctgaca
    89461 agtgcgcttt cttaatgcct gatgcgctac gcttatctgg cctacaaagt cctgcagatt
    89521 caatatatta caggtac
//
LOCUS       DAZFJF010000006        88151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFJF010000006 DAZFJF010000000
VERSION     DAZFJF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 88151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.006"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            140..583
                     /gene="cmtB"
                     /locus_tag="ACHKAU_000447"
     CDS             140..583
                     /gene="cmtB"
                     /locus_tag="ACHKAU_000447"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO5529579.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            611..1999
                     /gene="cmtA"
                     /locus_tag="ACHKAU_000448"
     CDS             611..1999
                     /gene="cmtA"
                     /locus_tag="ACHKAU_000448"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO5529580.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            2014..3291
                     /gene="yggP"
                     /locus_tag="ACHKAU_000449"
     CDS             2014..3291
                     /gene="yggP"
                     /locus_tag="ACHKAU_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5529581.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3288..4253
                     /gene="glpX"
                     /locus_tag="ACHKAU_000450"
     CDS             3288..4253
                     /gene="glpX"
                     /locus_tag="ACHKAU_000450"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5529582.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4275..4784
                     /gene="fumE"
                     /locus_tag="ACHKAU_000451"
     CDS             4275..4784
                     /gene="fumE"
                     /locus_tag="ACHKAU_000451"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO5529583.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4781..5494
                     /locus_tag="ACHKAU_000452"
     CDS             4781..5494
                     /locus_tag="ACHKAU_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO5529584.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5466..6143)
                     /locus_tag="ACHKAU_000453"
     CDS             complement(5466..6143)
                     /locus_tag="ACHKAU_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5529585.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6137..6814)
                     /locus_tag="ACHKAU_000454"
     CDS             complement(6137..6814)
                     /locus_tag="ACHKAU_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5529586.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6802..7509)
                     /locus_tag="ACHKAU_000455"
     CDS             complement(6802..7509)
                     /locus_tag="ACHKAU_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO5529587.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7510..8088)
                     /locus_tag="ACHKAU_000456"
     CDS             complement(7510..8088)
                     /locus_tag="ACHKAU_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO5529588.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8111..8542)
                     /locus_tag="ACHKAU_000457"
     CDS             complement(8111..8542)
                     /locus_tag="ACHKAU_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO5529589.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8914..9933
                     /gene="epd"
                     /locus_tag="ACHKAU_000458"
     CDS             8914..9933
                     /gene="epd"
                     /locus_tag="ACHKAU_000458"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO5529590.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9983..11146
                     /gene="pgk"
                     /locus_tag="ACHKAU_000459"
     CDS             9983..11146
                     /gene="pgk"
                     /locus_tag="ACHKAU_000459"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO5529591.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11361..12440
                     /gene="fbaA"
                     /locus_tag="ACHKAU_000460"
     CDS             11361..12440
                     /gene="fbaA"
                     /locus_tag="ACHKAU_000460"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5529592.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12504..12602)
                     /locus_tag="ACHKAU_000461"
                     /pseudo
     CDS             complement(12504..12602)
                     /locus_tag="ACHKAU_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12798..13658
                     /gene="mscS"
                     /locus_tag="ACHKAU_000462"
     CDS             12798..13658
                     /gene="mscS"
                     /locus_tag="ACHKAU_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO5529593.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13797..14432
                     /gene="argO"
                     /locus_tag="ACHKAU_000463"
     CDS             13797..14432
                     /gene="argO"
                     /locus_tag="ACHKAU_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO5529594.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14525..15265
                     /gene="yggE"
                     /locus_tag="ACHKAU_000464"
     CDS             14525..15265
                     /gene="yggE"
                     /locus_tag="ACHKAU_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO5529595.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15432..16328
                     /gene="ygfI"
                     /locus_tag="ACHKAU_000465"
     CDS             15432..16328
                     /gene="ygfI"
                     /locus_tag="ACHKAU_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5529596.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16325..17803)
                     /gene="scpC"
                     /locus_tag="ACHKAU_000466"
     CDS             complement(16325..17803)
                     /gene="scpC"
                     /locus_tag="ACHKAU_000466"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO5529597.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17827..18612)
                     /gene="scpB"
                     /locus_tag="ACHKAU_000467"
     CDS             complement(17827..18612)
                     /gene="scpB"
                     /locus_tag="ACHKAU_000467"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO5529598.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18623..19618)
                     /gene="meaB"
                     /locus_tag="ACHKAU_000468"
     CDS             complement(18623..19618)
                     /gene="meaB"
                     /locus_tag="ACHKAU_000468"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO5529599.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19611..21755)
                     /gene="scpA"
                     /locus_tag="ACHKAU_000469"
     CDS             complement(19611..21755)
                     /gene="scpA"
                     /locus_tag="ACHKAU_000469"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO5529600.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21959..22852)
                     /gene="argP"
                     /locus_tag="ACHKAU_000470"
     CDS             complement(21959..22852)
                     /gene="argP"
                     /locus_tag="ACHKAU_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO5529601.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22994..23179
                     /locus_tag="ACHKAU_000471"
     CDS             22994..23179
                     /locus_tag="ACHKAU_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529602.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23235..23894
                     /gene="rpiA"
                     /locus_tag="ACHKAU_000472"
     CDS             23235..23894
                     /gene="rpiA"
                     /locus_tag="ACHKAU_000472"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO5529603.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24150..25382
                     /gene="serA"
                     /locus_tag="ACHKAU_000473"
     CDS             24150..25382
                     /gene="serA"
                     /locus_tag="ACHKAU_000473"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5529604.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25611..25670
                     /gene="ibsC"
                     /locus_tag="ACHKAU_000474"
     CDS             25611..25670
                     /gene="ibsC"
                     /locus_tag="ACHKAU_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO5529605.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            26003..26062
                     /locus_tag="ACHKAU_000475"
     CDS             26003..26062
                     /locus_tag="ACHKAU_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5529606.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26163..26765)
                     /gene="fau"
                     /locus_tag="ACHKAU_000476"
     CDS             complement(26163..26765)
                     /gene="fau"
                     /locus_tag="ACHKAU_000476"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO5529607.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26786..26969)
                     /gene="ssrS"
                     /locus_tag="ACHKAU_000477"
     ncRNA           complement(26786..26969)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKAU_000477"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(27011..27340)
                     /gene="zapA"
                     /locus_tag="ACHKAU_000478"
     CDS             complement(27011..27340)
                     /gene="zapA"
                     /locus_tag="ACHKAU_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5529608.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27508..28086
                     /gene="ygfB"
                     /locus_tag="ACHKAU_000479"
     CDS             27508..28086
                     /gene="ygfB"
                     /locus_tag="ACHKAU_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO5529609.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28112..29437
                     /gene="pepP"
                     /locus_tag="ACHKAU_000480"
     CDS             28112..29437
                     /gene="pepP"
                     /locus_tag="ACHKAU_000480"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO5529610.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29434..30612
                     /gene="ubiH"
                     /locus_tag="ACHKAU_000481"
                     /gene_synonym="visB"
     CDS             29434..30612
                     /gene="ubiH"
                     /locus_tag="ACHKAU_000481"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO5529611.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30635..31837
                     /gene="ubiI"
                     /locus_tag="ACHKAU_000482"
                     /gene_synonym="visC"
     CDS             30635..31837
                     /gene="ubiI"
                     /locus_tag="ACHKAU_000482"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO5529612.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31911..31994)
                     /locus_tag="ACHKAU_000483"
     CDS             complement(31911..31994)
                     /locus_tag="ACHKAU_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529613.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32285..33379
                     /gene="gcvT"
                     /locus_tag="ACHKAU_000484"
     CDS             32285..33379
                     /gene="gcvT"
                     /locus_tag="ACHKAU_000484"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO5529614.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33403..33792
                     /gene="gcvH"
                     /locus_tag="ACHKAU_000485"
     CDS             33403..33792
                     /gene="gcvH"
                     /locus_tag="ACHKAU_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO5529615.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33911..36784
                     /gene="gcvP"
                     /locus_tag="ACHKAU_000486"
     CDS             33911..36784
                     /gene="gcvP"
                     /locus_tag="ACHKAU_000486"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO5529616.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            37045..37788
                     /gene="ygfF"
                     /locus_tag="ACHKAU_000487"
     CDS             37045..37788
                     /gene="ygfF"
                     /locus_tag="ACHKAU_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5529617.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37845..39278)
                     /gene="bglA"
                     /locus_tag="ACHKAU_000488"
     CDS             complement(37845..39278)
                     /gene="bglA"
                     /locus_tag="ACHKAU_000488"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO5529618.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            39323..39634
                     /gene="yqfB"
                     /locus_tag="ACHKAU_000489"
     CDS             39323..39634
                     /gene="yqfB"
                     /locus_tag="ACHKAU_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO5529619.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39798..40457
                     /gene="yqfA"
                     /locus_tag="ACHKAU_000490"
     CDS             39798..40457
                     /gene="yqfA"
                     /locus_tag="ACHKAU_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO5529620.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40653..41633)
                     /gene="ygfZ"
                     /locus_tag="ACHKAU_000491"
     CDS             complement(40653..41633)
                     /gene="ygfZ"
                     /locus_tag="ACHKAU_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO5529621.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41876..42142
                     /gene="sdhE"
                     /locus_tag="ACHKAU_000492"
     CDS             41876..42142
                     /gene="sdhE"
                     /locus_tag="ACHKAU_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO5529622.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            42123..42530
                     /gene="ygfX"
                     /locus_tag="ACHKAU_000493"
     CDS             42123..42530
                     /gene="ygfX"
                     /locus_tag="ACHKAU_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO5529623.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42570..43091)
                     /gene="fldB"
                     /locus_tag="ACHKAU_000494"
     CDS             complement(42570..43091)
                     /gene="fldB"
                     /locus_tag="ACHKAU_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO5529624.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            43203..44099
                     /gene="xerD"
                     /locus_tag="ACHKAU_000495"
     CDS             43203..44099
                     /gene="xerD"
                     /locus_tag="ACHKAU_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO5529625.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            44124..44834
                     /gene="dsbC"
                     /locus_tag="ACHKAU_000496"
     CDS             44124..44834
                     /gene="dsbC"
                     /locus_tag="ACHKAU_000496"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO5529626.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            44840..46573
                     /gene="recJ"
                     /locus_tag="ACHKAU_000497"
     CDS             44840..46573
                     /gene="recJ"
                     /locus_tag="ACHKAU_000497"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO5529627.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46664..47762
                     /gene="prfB"
                     /locus_tag="ACHKAU_000498"
     CDS             join(46664..46738,46740..47762)
                     /gene="prfB"
                     /locus_tag="ACHKAU_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO5529628.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47772..49289
                     /gene="lysS"
                     /locus_tag="ACHKAU_000499"
     CDS             47772..49289
                     /gene="lysS"
                     /locus_tag="ACHKAU_000499"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5529629.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49332..49880)
                     /gene="idi"
                     /locus_tag="ACHKAU_000500"
     CDS             complement(49332..49880)
                     /gene="idi"
                     /locus_tag="ACHKAU_000500"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO5529630.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            49935..50006
                     /locus_tag="ACHKAU_000501"
     CDS             49935..50006
                     /locus_tag="ACHKAU_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529631.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(50003..50128)
                     /gene="yqfG"
                     /locus_tag="ACHKAU_000502"
     CDS             complement(50003..50128)
                     /gene="yqfG"
                     /locus_tag="ACHKAU_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO5529632.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(50130..51578)
                     /gene="uacT"
                     /locus_tag="ACHKAU_000503"
     CDS             complement(50130..51578)
                     /gene="uacT"
                     /locus_tag="ACHKAU_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO5529633.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            52014..53933
                     /gene="ygfT"
                     /locus_tag="ACHKAU_000504"
     CDS             52014..53933
                     /gene="ygfT"
                     /locus_tag="ACHKAU_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO5529634.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53933..54421
                     /gene="ygfS"
                     /locus_tag="ACHKAU_000505"
     CDS             53933..54421
                     /gene="ygfS"
                     /locus_tag="ACHKAU_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5529635.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(54457..55824)
                     /gene="ghxQ"
                     /locus_tag="ACHKAU_000506"
                     /gene_synonym="ygfQ"
     CDS             complement(54457..55824)
                     /gene="ghxQ"
                     /locus_tag="ACHKAU_000506"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO5529636.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55860..57176)
                     /gene="guaD"
                     /locus_tag="ACHKAU_000507"
     CDS             complement(55860..57176)
                     /gene="guaD"
                     /locus_tag="ACHKAU_000507"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO5529637.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(57194..58594)
                     /gene="xanQ"
                     /locus_tag="ACHKAU_000508"
     CDS             complement(57194..58594)
                     /gene="xanQ"
                     /locus_tag="ACHKAU_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO5529638.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(58759..61629)
                     /gene="xdhD"
                     /locus_tag="ACHKAU_000509"
     CDS             complement(58759..61629)
                     /gene="xdhD"
                     /locus_tag="ACHKAU_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO5529639.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(61626..62405)
                     /gene="ygfM"
                     /locus_tag="ACHKAU_000510"
     CDS             complement(61626..62405)
                     /gene="ygfM"
                     /locus_tag="ACHKAU_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO5529640.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(62456..63784)
                     /gene="ssnA"
                     /locus_tag="ACHKAU_000511"
     CDS             complement(62456..63784)
                     /gene="ssnA"
                     /locus_tag="ACHKAU_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO5529641.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(63787..66885)
                     /gene="ygfK"
                     /locus_tag="ACHKAU_000512"
     CDS             complement(63787..66885)
                     /gene="ygfK"
                     /locus_tag="ACHKAU_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO5529642.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(67207..67785)
                     /gene="mocA"
                     /locus_tag="ACHKAU_000513"
     CDS             complement(67207..67785)
                     /gene="mocA"
                     /locus_tag="ACHKAU_000513"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO5529643.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            67888..68658
                     /gene="yqeC"
                     /locus_tag="ACHKAU_000514"
     CDS             67888..68658
                     /gene="yqeC"
                     /locus_tag="ACHKAU_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO5529644.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            68706..70331
                     /gene="yqeB"
                     /locus_tag="ACHKAU_000515"
     CDS             68706..70331
                     /gene="yqeB"
                     /locus_tag="ACHKAU_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO5529645.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(70372..71304)
                     /gene="arcC"
                     /locus_tag="ACHKAU_000516"
     CDS             complement(70372..71304)
                     /gene="arcC"
                     /locus_tag="ACHKAU_000516"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5529646.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(71352..72737)
                     /gene="hyuA"
                     /locus_tag="ACHKAU_000517"
     CDS             complement(71352..72737)
                     /gene="hyuA"
                     /locus_tag="ACHKAU_000517"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO5529647.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(72790..74001)
                     /gene="ygeY"
                     /locus_tag="ACHKAU_000518"
     CDS             complement(72790..74001)
                     /gene="ygeY"
                     /locus_tag="ACHKAU_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO5529648.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(74059..75255)
                     /gene="dpaL"
                     /locus_tag="ACHKAU_000519"
     CDS             complement(74059..75255)
                     /gene="dpaL"
                     /locus_tag="ACHKAU_000519"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO5529649.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(75313..76500)
                     /gene="ygeW"
                     /locus_tag="ACHKAU_000520"
     CDS             complement(75313..76500)
                     /gene="ygeW"
                     /locus_tag="ACHKAU_000520"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO5529650.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            76979..78757
                     /gene="ygeV"
                     /locus_tag="ACHKAU_000521"
     CDS             76979..78757
                     /gene="ygeV"
                     /locus_tag="ACHKAU_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO5529651.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(78797..79276)
                     /gene="xdhC"
                     /locus_tag="ACHKAU_000522"
     CDS             complement(78797..79276)
                     /gene="xdhC"
                     /locus_tag="ACHKAU_000522"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO5529652.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(79273..80151)
                     /gene="xdhB"
                     /locus_tag="ACHKAU_000523"
     CDS             complement(79273..80151)
                     /gene="xdhB"
                     /locus_tag="ACHKAU_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO5529653.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(80162..82459)
                     /gene="xdhA"
                     /locus_tag="ACHKAU_000524"
     CDS             complement(80162..82459)
                     /gene="xdhA"
                     /locus_tag="ACHKAU_000524"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO5529654.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            82874..83629
                     /gene="actS"
                     /locus_tag="ACHKAU_000525"
     CDS             82874..83629
                     /gene="actS"
                     /locus_tag="ACHKAU_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO5529655.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            83708..83781
                     /locus_tag="ACHKAU_000526"
     tRNA            83708..83781
                     /locus_tag="ACHKAU_000526"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83740..83742,aa:Gly,seq:ccc)
     gene            83916..85523
                     /locus_tag="ACHKAU_000527"
     CDS             83916..85523
                     /locus_tag="ACHKAU_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5529656.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPSASYEELREHLKTIVEWELGVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLKGDNSGLLSYLE
                     PETGSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTAGKPYHYYVTALAAITGARLNEVAQLQVKDVRATEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTAGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGLNG"
     gene            85516..86175
                     /locus_tag="ACHKAU_000528"
     CDS             85516..86175
                     /locus_tag="ACHKAU_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFO5529657.1"
                     /translation="MAEYYPAVFEAEAFNCPHCGVYARQFWRRMYGSARELAQRTTEF
                     RMSTCSHCGDDAYWYDGNMIIPAAGNVELPNPDMPDNCKSDYMEARSIINLSPKGAAA
                     LLRLCLQKLMVHLGEPGENINKDIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISL
                     DDDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAAN
                     E"
     gene            87084..87812
                     /locus_tag="ACHKAU_000529"
     CDS             87084..87812
                     /locus_tag="ACHKAU_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5529658.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPAIVDKLRGILIA
                     FTGTDQQKQEHLLKQAQSGKLEGL"
     gene            87814..88014
                     /locus_tag="ACHKAU_000530"
     CDS             87814..88014
                     /locus_tag="ACHKAU_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529659.1"
                     /translation="MIDPRTPIGRATLRYRGLPTRHLLSLLRLGVDNPDRPYYSRDEL
                     IAMLVDRDLNNQLRRAFAKLES"
BASE COUNT        21420 a        21961 c        23299 g        21471 t
ORIGIN      
        1 taactcaatg ttatataaga aattaataat tcactgtttt caaaacatcg gtttccctgc
       61 tcaattgctt tcattaaacc gcacgagtat tttattttta ccgccggacg gtaccctaaa
      121 aataaaataa ggataattta tgcggcttag tgattatttt ccagaatcat caatctcagt
      181 tatacattca gcaaaagatt ggcaggaagc tatcgatttc tcgatggtat cattgctgga
      241 taaaaactat atcagcgaga attacattca ggcaattaaa gattccacca ttaacaatgg
      301 cccttattat attctcgcac caggcgtggc aatgcctcat gcgcgaccgg aatgtggggc
      361 gcttaaaacc gggatgtctt tgacattact tgaacaaggt gtttattttc cggggaatga
      421 cgaaccaatt aaattactca tcggactctc tgctgccgat gccgattcgc acattggcgc
      481 tattcaggcg ttaagtgaat tactgtgcga agaagaaata ctcgaacaac tcttaacagc
      541 atcatcagaa aaacaattag cggacattat cagccgcgga taattactct ctcctctaca
      601 ggataatatt atggaaaaca agtctgctcg tgcaaaggtc caggcttttg ggggcttttt
      661 gactgcaatg gtcatcccca atattggtgc ttttattgcc tggggtttta ttactgcgtt
      721 atttattccc accggttggc tgcctaacga acatttcgcc aaaattgtcg gcccgatgat
      781 tacctattta ttgcccgtga tgattggttc tacaggtggt catctggtcg gcggtaaacg
      841 cggggcggtc atgggcggaa taggtactat tggtgtgatc gttggcgcag agatcccgat
      901 gttccttggc tcaatgatca tggggccgct cggtgggttg gtcataaaat atgtcgataa
      961 ggcactggaa aaacgcatac ctgccggttt tgagatggtt atcaataact tctcattagg
     1021 tatcgcgggg atgctccttt gtctgctggg ttttgaagtg atcggcccgg cggtgttaat
     1081 tgccaatact ttcgtcaaag agtgtatcga ggcgctggta catgcgggtt atctgcctct
     1141 gctgtcagtc atcaatgaac cggcgaaagt gcttttcctt aataatgcga tcgatcaggg
     1201 cgtctattat ccgctgggaa tgcaacaggc ttcggttaac ggtaaatcca tcttctttat
     1261 ggtggcctct aacccaggtc cgggcctggg gctgctgctg gcgtttacct tgtttggtaa
     1321 agggatgagt aaacgttctg cgcccggggc gatgattatt cacttcctcg gtgggatcca
     1381 cgaactgtat ttcccatatg tgctgatgaa gccgctgact attattgcca tgattgcggg
     1441 cggtatgtct ggtacctgga tgtttaactt actggacggt ggtctggtgg ctggcccaag
     1501 tccggggtct atctttgctt acctggcact gacgccgaaa ggctcgttcc tggcgacaat
     1561 tgccggtgtt acggtaggta ccctggtgtc ctttgctatt acttcgctga tactgaagat
     1621 ggaaaaaacg gtggaaacgg agagcgaaga tgagtttgct cagtcagcca atgcggttaa
     1681 ggcgatgaaa caagagggtg cattctcgtt aagcagggtt aagcgtatcg cctttgtttg
     1741 cgatgcgggg atgggctcca gtgcgatggg cgcgaccacc ttccgtaaac gcctggaaaa
     1801 agcggggctg gcaatagaag taaaacatta cgcgatagaa aacgtgcctg cggatgccga
     1861 tatcgtcgtc actcacgcca gtctggaagg gcgcgtgaaa cgtgtgacgg ataaaccgct
     1921 gatattgatt aataactata ttggcgatcc aaaactcgac actttattta atcaattaac
     1981 cgccgaacat aaacactgat tacagaggta aaaatgaaaa ccaaagttgc tgctatttat
     2041 ggcaagcggg atgtccgtct gcgcgaattt gaactgccag aaattaccga taatgaactg
     2101 ttagtgagtg taatttctga cagcgtctgt ttatcgacct ggaaagcggc gttactcggt
     2161 agtgaacata aacgcgtacc cgacgattta gaaaatcatc cggtcattac cgggcatgaa
     2221 tgtgccgggg ttattgtcga agtgggtaaa aatctcactg gcaaatataa aaaaggccag
     2281 cgttttgtat tgcaaccggc gatggggtta ccaagcggat attcagcagg ctacagctac
     2341 gaatattttg gcggtaacgc cacctatatg attattcccg aaatcgccat taatttgggc
     2401 tgcgtattac cgtatcacgg ctcttatttt gctgcggcgt cgctggcaga gcctatgtgc
     2461 tgcattattg gtgcttatca tgccaattat cacaccacgc aatatgttta tgagcatcgc
     2521 atgggcgtca aacctggcgg caatattgca ctgctggcgt gtgcgggtcc gatgggcatt
     2581 ggcgctatcg attacgccat taacggtggc atacaaccgt cacgggtggt ggtggtcgat
     2641 atcgacgaca aacgcctggc gcaggtgcag aagctgctgc cggtggatct ggcggccagt
     2701 aaaggcattg agctggtgta tgtgaatacc aaagggatga gcgatccggt ccagacgctg
     2761 cgggcgctga caggagatgt cgggttcgat gacatttttg tttatgcggc ggtgcctgct
     2821 gtcgttgaga tggctgacga attactggcg gaagatggct gtctgaactt ctttgccggg
     2881 ccgacggata aaaacttcaa agtgccgttt aatttctaca acgtccatta caatagcacg
     2941 cacgtagtcg gtacatccgg cggttcaacg gacgacatga aagaggcgat tgcgttaagc
     3001 gccacggggc agttgcagcc gtcctttatg gtcacgcata tcggtgggct ggatgcggtg
     3061 ccagataccg tgctcaatct gccggatatc cctggcggta aaaaactcat ttataacggc
     3121 gtgaccatgc cgctcactgc cattgccgat tttgccgaaa aaggcaaaac cgatccgctg
     3181 tttaaagagt tggcgcggct ggttgaggaa acgcacggca tctggaatga acaggccgag
     3241 aaatatctgc tggcacaatt tggcgttgat atcggggagg ccgcgcaatg atgtccctgg
     3301 cgtggccatt atttcgcgtt acggaacagg cagcgttggc tgcctggccg caaaccggat
     3361 gtggcgacaa aaataaaatt gatggcctgg cggtcaccgc gatgcgccag gcattaaacg
     3421 acgtcgcttt tcgtggacga gtggttatcg gcgaaggaga aattgaccat gcgccgatgc
     3481 tgtggattgg cgaagaggtg ggcaaaggcg atgggccaga agtcgatatc gcggttgacc
     3541 cgattgaagg cacgcggatg gtagcgatgg ggcagagcaa tgcgctggcg gtcatggctt
     3601 tcgcccctcg agatagcctg ttacatgcgc ccgatatgta tatgaaaaag ctggttgtta
     3661 atcgactggc tgccggggcg attgatctgt ctttgcctct ggcggacaac ttgcgtaacg
     3721 tggcgcgggc gttaggtaaa ccgctggaca agctgcgcat ggttacgctg gataaaccgc
     3781 gcctgagtgc cgcgattgaa gaagcaacac agctgggcgt gaaggttttc gctctgccgg
     3841 atggcgatgt cgccgccagc gtgctgacct gctggcagga taatccttac gacgtgatgt
     3901 acaccatcgg cggtgcgcca gaaggcgtga tttctgcctg tgccgttaaa gcattaggtg
     3961 gggatatgca ggccgagctg attgactttt gtcaggctaa aggggattac acggaaaatc
     4021 ggcagattgc agagcaggag cgcaagcgtt gtaaggcaat gggcgttgac gttaaccgtg
     4081 tgtactcgct cgatgaactg gtgaggggga acgatatcct ctttagcgct acgggcgtga
     4141 cgggcggcga gttggtgaac ggtatccaac agacggcgaa tggggtgcgg acgcagacat
     4201 tactgatcgg cggcgcggac caaacgtgta atataataga ctccctgcat tgatggtgat
     4261 ctatcggaag accaatggcg acgctgacag aagatgatgt gcttgagcaa ctggatgcac
     4321 aggacaattt attttcattt atgaaaactg cgcattctat tttgctccag gggatacgcc
     4381 agtttctgcc gtcgctgttt gtcgataacg atgaagagat cgtcgaatat gcagtgaagc
     4441 cgttactcgc ccaaagcggc ccgcttgacg atattgatgt tgcgctgcgt ttgatttatg
     4501 cgctggggaa aatggataaa tggctgtatg ccgatatcac gcacttttcc cagtactggc
     4561 attacctgaa cgaacaggat gaaacgcccg gatttgccga tgacatgacc tgggatttta
     4621 tcagcaacgt caatagcata acctgcaatg cgacgctcta tgacgcgtta aaagcgatga
     4681 agttcgccga tttcgccgtc tggtcagagg cgcgttttag cggaatggtc aaaacggcgc
     4741 tgacgctggc agtaacgacc accttaaagg aattaacgcc gtgaaaattg aattaacggt
     4801 gaatgggctg aacgtgcaag cgcagtacca tgatgaagag atagaacgcg tgcataaacc
     4861 gttgctgcgt atgctggcgg ccttacagac ggtaagtccg cagcgacgta cggtggtttt
     4921 tctttgcgct ccgccgggga cgggtaaatc taccttaact accttctggg aatatctcgc
     4981 gcaacaagac cctgaattac ccgctatcca gacgctcccg atggatggtt ttcaccatta
     5041 caatagctgg ctggatgcgc atcaattgcg ccccttcaaa ggcgcaccag agacatttga
     5101 cgttgcgaaa ctggcggaaa atctgtgcca ggtagtggaa ggggattgta cgtggccgca
     5161 gtatgatcga caaaagcatg atcctgttga agatgcgttg cacgttaccg caccactcgt
     5221 catcgtcgaa ggaaactggt tgttgctgga cgacgagaag tggtgccagc ttgcgcaatt
     5281 ttgtgatttc tcgatcttta tcaacgcgcc agctactgcg ttgcgggaac gactggtagg
     5341 gcgcaagctg gcgggagggg tatcgctggc agacgcagaa gctttttatg accgtaccga
     5401 cgggccgaat gttcgccggg ggctggaaga gagtctgcct gcaaatttga ccttaatgat
     5461 gaccgctacg ggtgaatatc gtctggtgga ttaacgttcc tgattagacc atcttccagt
     5521 cgcaccacac gagagaaatg acgccgggta aatgcggcgt catgactgat tgctacgacg
     5581 ctcgtgcctc gctggccgca cttctccagc cagctttcaa acacgctcaa ccaattttcg
     5641 tcaaaatccc gactcggctc gtccagcagc aatagtggcg gagagagtgc ttccaggcag
     5701 gcgacggcga ccatccgtcg ctgcgcacta tgcagatcca acgggtgcga gttggcagtg
     5761 tctgctaact gacaacattg cagggcagca tgggtacgtt gagtgatttc atccgccggg
     5821 catttttgca gtttcaaacc aaatgcgatc tcgtcagcga cggtgctgtg aaaaagttgg
     5881 ttttccgctt cctgaaacag tacgccaacc tttgccgcgc gctggcggtt tttcaggttt
     5941 ttcatcgcct gttgttgcag cataaccgta ccggctgtgg gcgtaagaag acctgccatt
     6001 actcgtaaca gcgtcgattt tcccgcgcca ttgtcgccgg tcagcgccaa ccattcacct
     6061 tgtttgagtt gcagagaaat gtcgcaaaga caatccgttg ccgcgccagg ccagcgataa
     6121 gatatcttat ttaacgttaa catagtggct ggagcgctcc gttttgcaat tgccacgctt
     6181 gctggcagta gttcaggaaa ggtgtgcgat gacgttcaaa aagaataatc agcgaaccac
     6241 gttcaaacgc ccagtgttgt aagcgctgca acagcatttc gctggcctgc ggagtcaggc
     6301 ggctaaaggc ttcatcaaga attaagagct ttggctgcat cgcgatggcg caggcgatga
     6361 ccacgcgctg tgtctctcca ccggagagtg ttgccgggtg acgatggcgc aacggctggc
     6421 actcggtcag agtcagggcg gcatcaatcc gcgccataat ctctgcttca gcaagacaca
     6481 gattttctgg cccaaaggca acttcttctt ccacgctaaa ggtacagccg gagagctgca
     6541 aatagggcga ttgctggacg agctggatgg tggcggattg ttcattaagc gggagatgcc
     6601 caatgggcgt accgaggagc gtccccgtgc ctgttatctc tcctggcaga aagtccggat
     6661 accagcccgc catcagctgt gccagcgtac ttttgccact gccgttgtca ccaaagatgg
     6721 cgaccatccc cggtttgaca tagtgaaagt cataacagaa cggaggatgc gttgagtgtg
     6781 tcgggcaata acggaactgt tctaacgtaa ccatatccac gctccgaatt ctgtcagcat
     6841 cagcaagatc atggtgtaac gcgctacgcg ttgtagggtg ctatcggctg gtgcccacaa
     6901 cgttgttcgt cggttgtgga tccgaaatgc ccgcatatcc agcgccgcac cacggacagc
     6961 cagatcgtta agcgcattgt gtgttagcgg aataattaac gcgggcatcg cacgtaaacg
     7021 ctgataccag ccctcatcca gcggcacgcc gcgggcacgc tgcgcttcat gaataatcgc
     7081 taattgccgt tttaactgct caacgaccaa taacggcccg gcaaacagat aagcgacgcc
     7141 tggcggcagg cgagaagcaa acagagcgcg aataaaacat tgcacgggga cgtactgcat
     7201 ccacaactgt gaggttgaaa caatcgccag aatccgcaac cagagcgtaa tagcatgcga
     7261 ccaccgttct ggcgagcgtg gcgtgccgct tagccattct ggtagccagc caccgtgtac
     7321 cagccacagc ccggctccca gcgagaacat cagccagacg acatattttg cccgccgctg
     7381 cgtggccttt agcgcaataa ggcagaaaaa ggtcgccgcg ctatagatcg gcaaaatggt
     7441 ctgcgcgggc aggataagcg tggtgcaggc cgccaatgcc cacagtgtta atgaggtaaa
     7501 tggatgcatt tagcgcacgg cagccattgc cggaaaatgg cgcgtggtgc gaatcggcag
     7561 ctggcgcagt aaaagccaga caatcaccgc tgtaagaatt ttatcgacca ggttcgcgcc
     7621 tatcaccgta atcgccaccg actccaccag gttttgcccc atcgagtgca tccaggcaac
     7681 gaacaaatcg gctccgctac cggtgacacc gccgaacagc gcagtacgca gcggtacagc
     7741 gacgaccgtt accgcaagcg taataatcac gccgctgaca acgacttttg gcagggtgcg
     7801 aaaccaaccc gctcgcgcca ggcaaccggc gaccaggccg atgaccatcg cgacgggtgc
     7861 aaatgcggcg gcgatcgggt tggtcagcaa tccccagagt aaattggtca gtaagccggt
     7921 cagcattccg atcaccggac caagcagtac cgcgctaata agtgtgccga tggagtcgag
     7981 aaatataggt aatttgacca tgctggccag ttgcccgccg atcatattga tggcgataga
     8041 tataacgatc aacaccagtg cctgactgga aaagtggcta cgcgccataa gaaaacctct
     8101 ttgaaagtag ttatttaacg ggggaaatat gcaactcgtc gtaaccagaa agcgcgcaag
     8161 gttggcggct aaacgccagt tcctcaaggc taccgatcac cagttcaagc gtggtccgca
     8221 cggtacagcc gggcatcata tgaccgccaa gcatcgtgcc gtgtggatcg gaaacgcaga
     8281 gatgaagatg ctcaccactc tgttccagcg tgccgtttag tgagatcact tcgaatgtac
     8341 cgttcaggag cgtggtgcct tcttgcccgg catagcgcaa ggcgacgtca gtcaggctac
     8401 cggtacagcc tgcaatccag gcggcatgaa gttgatgctg ctgtgcaaaa gcgtgtagct
     8461 gggaaaaaac ttcctgcccg gggaggaggc gcagtgcgta aaagcgtgcg gaggaagaat
     8521 atgggtaggg caatgtcatc attggagtac tccgaaactc ggacgtattg cggataatat
     8581 cgacgctgca tcagtcatat tctgatataa gtcagggttt gtgacggcac aacgaaaaga
     8641 ttttgctacg cttattcgtg attctgcaaa gtggaataaa ttccacaggg gcggggctga
     8701 aaacgtgacg taagtcagat aattgctttc tttgactgga caatcattcc ttttattcca
     8761 cgtttcgctt atcctagctg aagcgtttca gtcgattaaa tgttcgacaa ttaaccaatc
     8821 agtcgcagtt tgcgacaggt aaggtttccc cggacgattt gctggattac tctgtctggc
     8881 aaatttgctc agggaaaacc ttgcaggaga tctatgaccg tacgcgtagc gataaatggc
     8941 ttcggtcgca tcgggcgtaa tgtggttcgt gctttgtatg aatccggacg ccgggcggaa
     9001 attaccgtgg tggcaatcaa cgaactggcg gatgctgcgg gcatggcgca tttgttgaaa
     9061 tatgacacca gccatggccg ttttgcatgg gaagtacgac aggaacgcga tcaacttttt
     9121 gttggtgatg acgccatccg cgtattgcat gaacgttcac tgcaatcgct cccctggcgt
     9181 gaacttggcg ttgatgtagt cctcgactgc accggcgtat atggctcccg cgagcatggc
     9241 gaagcgcata ttgccgccgg ggccaaaaaa gtgctctttt cacatcctgg cagtaacgat
     9301 ctcgacgcga ccgttgttta cggcgtcaat caggatcaac ttcgtgcgga acaccgcatc
     9361 gtttctaacg cttcctgtac cacgaattgc ataattcccg tcatcaaatt gttagatgat
     9421 gcgtacggta ttgagtccgg cactgtgacc acaattcact ccgccatgca cgatcaacag
     9481 gttattgatg cataccatcc tgacctgcgt cgcacccggg cagccagcca gtcgatcatt
     9541 ccggtcgata ctaaactggc cgccggtatc acacgatttt ttccgcaatt taacgatcgc
     9601 tttgaagcga ttgcggtacg tgtgccaacc ataaatgtga cggcaatcga tttaagcgtg
     9661 acggtgaaga aacctgtaaa agccaatgaa gtcaacctgt tgctgcaaaa agcagcacaa
     9721 ggtgcatttc atggtatagt tgactatacg gaattgccgt tggtctctgt agattttaac
     9781 cacgatccgc acagtgccat tgtcgatggc acccaaaccc gggtcagtgg cgcacacctg
     9841 atcaaaacgt tggtctggtg cgataacgaa tggggctttg ctaaccgaat gctcgacacg
     9901 acgttagcta tggctactgt tgctttcagg taagacgcaa gcagcgtctg caaaactttt
     9961 agaatcaacg agaggattca ccatgtctgt aattaagatg accgatctgg atcttgctgg
    10021 gaaacgtgta tttatccgtg cggatctgaa cgtaccagta aaagacggga aagtaaccag
    10081 cgacgcgcgt atccgtgctt ctctgccgac catcgaactg gccctgaaac aaggcgcaaa
    10141 agtgatggta acttcccacc tgggtcgtcc taccgaaggc gagtacaacg aagaattctc
    10201 tctgctgccg gttgttaact acctgaaaga caaactgtct aacccggttc gcctggttaa
    10261 agattacctc gacggcgttg acgttgctga aggtgaactg gttgttctgg aaaacgttcg
    10321 cttcaacaaa ggcgagaaga aagacgacga aaccctgtcc aaaaaatacg ctgcactgtg
    10381 tgacgtgttc gtaatggacg cattcggtac tgctcaccgc gcgcaggctt ctactcacgg
    10441 tatcggtaaa ttcgctgacg ttgcgtgcgc aggcccgctg ctggcagctg aactggacgc
    10501 gctgggtaaa gcactgaaag aaccagctcg cccgatggtg gctatcgttg gtggttctaa
    10561 agtatctacc aaactgaccg ttctggactc cttgtctaaa atcgctgacc agctgattgt
    10621 tggtggtggt atcgctaaca cctttatcgc ggcacaaggc cacgatgtgg gtaaatccct
    10681 gtacgaagct gacctggttg acgaagctaa acgtctgctg accacctgca acatcccggt
    10741 tccgtctgat gttcgcgtag caaccgagtt ctctgaaact gcaccggcta ccctgaaatc
    10801 tgttaacgat gtgaaagctg acgagcagat cctggatatc ggtgatgctt ccgctcagga
    10861 actggctgaa atcctgaaga atgcgaaaac cattctgtgg aacggtccgg ttggcgtgtt
    10921 cgaattcccg aacttccgca aaggtactga aatcgtggct aacgctatcg cagacagcga
    10981 agcgttctcc atcgctggcg gcggcgacac tctggcagca atcgacctgt tcggcattgc
    11041 tgacaaaatc tcctacatct ccactggcgg cggcgcattc ctcgaattcg tggaaggtaa
    11101 agtactgcct gcagtagcga tgctcgaaga gcgcgctaag aagtaaaaaa tcacagggca
    11161 gggaaacctg cccttgtttc agcgcgcttt tagagcacgc attttttcat actctaaata
    11221 attcgagttg caggaaggcg acaagagagt gaatccccag gagcttacat aagtaagtga
    11281 ctggggtgag cgaacgcaga cgcagcacat gcaacttgaa gtatgatgag tataaggccc
    11341 gacgatacag gacaagagac atgtctaaga tttttgattt cgtaaaacct ggcgtaatca
    11401 ctggtgatga cgtacagaaa gttttccagg tagcaaaaga aaacaacttc gcactgccag
    11461 cagtaaactg cgtcggtact gactccatca acgccgtact ggaaactgct gctaaagtta
    11521 aagcgccggt tatcgttcag ttctccaacg gtggtgcttc ctttatcgct ggtaaaggcg
    11581 tgaaatctga cgttccgcag ggtgctgcta tcctgggcgc gatctctggt gcgcatcacg
    11641 ttcaccagat ggctgaacat tatggtgttc cggttatcct gcacactgac cactgcgcga
    11701 agaaactgct gccgtggatc gacggtctgc tggacgcggg tgaaaaacac ttcgcagcta
    11761 ccggtaagcc gctgttctct tctcacatga tcgacctgtc tgaagaatct ctgcaagaga
    11821 acatcgaaat ctgctctaaa tacctggagc gtatgtccaa aatcggcatg actctggaaa
    11881 tcgaactggg ttgcaccggt ggtgaagaag acggcgtgga caacagccac atggacgctt
    11941 ctgcactgta cacccagccg gaagacgttg attacgcata caccgagctg agcaaaatca
    12001 gcccgcgttt caccatcgct gcgtccttcg gtaacgtaca cggtgtttac aagccgggta
    12061 acgtggttct gactccgacc atcctgcgtg attctcagga atatgtttct aagaaacaca
    12121 acctgccgca caacagcctg aacttcgtat tccacggtgg ttccggttct actgctcagg
    12181 aaatcaaaga ctccgtaagc tacggcgtag tgaaaatgaa catcgatacc gatacccaat
    12241 gggcaacctg ggaaggcgtt ctgaactact acaaagcgaa cgaagcttat ctgcagggtc
    12301 agctgggtaa cccgaaaggc gaagatcagc cgaacaagaa atactacgat ccgcgcgtat
    12361 ggctgcgtgc cggtcagact tcgatgatcg ctcgtctgga aaaagcattc caggaactga
    12421 acgcgatcga cgttctgtaa gtcattcctt tctgcttatc tcaaggcccg ctctgcgggt
    12481 ctttttttcg ccaaaagcaa aattgcctga tgcgctacgc ttatcaggcc tacacgtatc
    12541 ctgcaatata ttgaatttgc aggtttttgt aggccggata aggcgttcac gccgcatccg
    12601 gcatgacaaa gcgcacattg atatctgtct gagccccgct ctgcggggat tttttcgcca
    12661 aaagcaaggt gattcagatg agaaatctgt gatctatttg gcaaaattat gctttattgt
    12721 ttacccttgt cagactgccc gtcataaggc ggcggagtgt atttctccat tttgagtcag
    12781 ttgaaaagga atattgaatg gaagatttga atgttgtcga tagcataaac ggcgcgggaa
    12841 gctggctggt agctaaccag gcgctgctgc taagttatgc agtaaacatc gtggcggcac
    12901 tcgcgatcat catcgttggt ttgattatcg cgcggatgat ttccaacgcg gtgaatcgcc
    12961 tgatgatctc ccgtaaaatc gatgccactg ttgctgattt tctttctgca ttagtccgtt
    13021 acggtattat cgcctttacg ctaatcgcag ccctgggacg cgtgggtgta caaacagcgt
    13081 cagtcattgc tgtactcggt gccgcaggct tagctgttgg tctggctttg caggggtcac
    13141 tttctaacct ggccgctggc gtgttacttg tcatgttccg cccgttccgt gccggagaat
    13201 atgttgacct cggcggcgta gccggtactg tgctgagtgt gcagattttc tccaccacca
    13261 tgcgtactgc agacggtaaa attatcgtta ttccgaacgg taaaattatt gccggaaata
    13321 ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt tattattggc gtggcgtatg
    13381 attccgatat cgatcaggtt aagcagatcc tgaccaatat tatccagtct gaagatcgca
    13441 ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact tggtgcatcg tcgattaatt
    13501 tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa cgtgtactgg gatgtgctgg
    13561 agcgtattaa acgtgaattt gatgccgccg gtatcagctt cccgtacccg caaatggatg
    13621 tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc aacgctgagg ccagataata
    13681 ctctctggcc tctctcttat tagttattct gattgccaat taatattatc aatttccgct
    13741 aataacaatc ccgcgatata gtctctgcat cagatactta attcggaata tccaacgtgt
    13801 tttcttatta ctttcaaggt cttgctcttg gggcagctat gattctgccg ctcggcccac
    13861 aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta ccacattatg attgccttac
    13921 tttgcgctat cagcgatttg gtcctgattt gcgcggggat ttttggtggt agcgcgttat
    13981 tgatgcagtc gccgtggttg ctggcgctgg tcacctgggg cggcgtagtc ttcttgctgt
    14041 ggtatggttt tggcgctttt aaaacagcga tgagtagtaa tattgagtta gctagtgccg
    14101 aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat gttggcagtg acctggctga
    14161 atccgcatgt ttacctggat acttttgttg tactgggcag ccttggcggg cagcttgacg
    14221 tcgaaccaaa acgttggttt gcgctcggga caattagcgc ctctttcctg tggttctttg
    14281 gtctggctat tctcgcagcc tggctggcac cgcgcctgcg cacggcaaaa tcacagcgca
    14341 ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc cttgcagctg gcgagagacg
    14401 gtattgctca tgcacaagcc ttgttcagtt aggcgctatc tgatggaaaa ataaaacaga
    14461 ggcgctaagc ttgcctccag aggtcctgaa ttttgggcaa gtcaattaac acggagagac
    14521 taacgtgaag ttcaaagtta tcgccctggc ggcattaatg ggtattagcg ggatggcagc
    14581 gcaggctaac gaattgccgg atggaccgca tattgtcacc tccggtacgg caagtgtgga
    14641 tgcggtgcca gacattgcca ctcttgcgat tgaagttaac gtggccgcga aggatgccgc
    14701 tacagccaag aaacaggcag atgagcgcgt cgcacaatac atttccttcc ttgaactcaa
    14761 tcagatcgcg aaaaaagata tcagctcagc gaatttacgc acccagccag attatgatta
    14821 tcaggatggt aaaagtatcc tgaaaggcta ccgcgctgtg agaacggtgg aagtcacgct
    14881 ccgtcagtta gacaaactga attccttgct ggatggcgcg ctgaaggcgg gtcttaacga
    14941 aattcgttct gtgtcgctgg gcgtggcgca gccggatgcc tataaagaca aagcgcgtaa
    15001 ggcagcgatt gataacgcga ttcatcaggc gcaggaactg gcgaacggct ttcatcgtaa
    15061 actggggccg gtatatagcg tgcgctacca tgtttccaac tatcagccca gcccaatggt
    15121 gcggatgatg aaagccgatg ccgcgccggt gtctgcccag gaaacttacg agcaggccgc
    15181 tattcagttt gatgatcagg tcgatgtggt cttccagtta gaacctgtgg atcaacaacc
    15241 cgctaaaaca cctgcagcac aataatactt gtagcccgac gtattcgtcg ggctaatgcc
    15301 tttacccgat attgcctcct ttttctgtaa ttttctgtaa aaccatgacg taatcaccta
    15361 tctttgctcg cggtttcatc aaggataggc tgactgaaac ggttaagcgg acaaggatgc
    15421 ggggaggggg gatggacatt tttatttcga aaaagatgcg caatttcatt ttattagcgc
    15481 aaaccaataa tattgcccgg gcggcagaaa aaatccatat gacggcttcg ccatttggta
    15541 aaagcattgc cgcgctggaa gaacaaattg gctatacgct atttacccgc aaagataata
    15601 atatcagcct caataaagcc ggtcaggaac tgtatcaaaa actgttcccg gtttatcaac
    15661 gactttctgc gattgataat gaaatccata actcagggcg tcgttcacga gaaattgtga
    15721 ttggcataga taatacttat ccgacaatta ttttcgatca gttgattagt ctcggagata
    15781 agtatgaagg cgtgaccgcc cagccagttg agttcagcga aaacggtgtt atcgataatc
    15841 tttttgaccg tcagttagat tttattatct ccccacagca tgtatctgca cgagttcagg
    15901 aactggaaaa cctgactatc agtgaattac caccgctgcg attgggcttt ctcgtttctc
    15961 gccgttatga agagcgtcaa gagcaagagc tgcttcagga gctgccctgg ctacaaatgc
    16021 gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc gaatatgcga ccttgtggga
    16081 tcaatcccac cattatttat cgtccctaca gttttatggc caaaatcagc gccgtggagc
    16141 gcggacattt tctgacggtg atcccgcatt ttgcctggcg actggtgaat ccggcaacgc
    16201 tgaaatattt tgatgcgccg cacaggccga tgtatatgca ggaatatctt tattcaatca
    16261 gaaatcatcg ttataccgcc acgatgcttc agcatattgc tgaagatcgt gacgggacaa
    16321 accattaacc cagcatcgag ccggttgcaa ttaaattacg gtgtaagtcg aagacgtggc
    16381 taagatcgtg gtgaatatgt ccgccgggcg cattttccag atagcgatgc agataatccc
    16441 gatacatagg atgtgcacaa ttatcaatga tagtgcgggc gcgttgaagc ggggaaagac
    16501 cgcgcagatc ggcgatccct tgttcggtga tgatcacttt gacgctgtgt tcgctgtgat
    16561 caacatggct gcacattggc acgacggttg agatcttgcc ttctttagca atcgacggag
    16621 ccataaagat cgacagatac gcgttgcgtt caaaatcacc gctgccgccg atgccgttca
    16681 tcagatcgac cccggctacg tgcgttgagt tggcatgccc gtaaatatca aactccaggc
    16741 cgacgttcag agcgatgacg cccagacgac ggatgatttc cgggttattg gaaatctcct
    16801 gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt gtcgtaaatc ttgcgcaggg
    16861 aatcggccga gattgtcagg ctggaggcgc tggccccgct gattttgccg gtttccagta
    16921 aatgcaccac cgattcctgt agcacttccg aatacatcat gaacggagga atttccgggt
    16981 tttcccccag acgcgccatt accgcattat tgatattgcc cacgccactt tgcagcggca
    17041 gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa taagaacgtg accacgttat
    17101 cggcaatctg ctggcacatg ggattttgct tatccagcat attaccggcg tcgggcaagt
    17161 tggtttccac gacggcgaca atctttttcg gatcgatttg cacatagcgg gtaccgacgc
    17221 gatccattgc atggaagatc gacacgctat tgcgccgcgg tggcgcgcca ggaatcacaa
    17281 tatctgccag ttctgcaacg cgcggatcgt gatagtgatt aagttcaatg atcactttct
    17341 tcgctcgcag cagccaggtc ggcgcattac cgatcccgct ggttaaccag actcgaccat
    17401 ccggtgccag tgccgatgct tcaatgacgg caacatcaat gtcgccgaag aaaccgtaat
    17461 tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa actcaccgcg ccctgattga
    17521 tctttttacg taaaccggac gatgtttgat atggcgcacg ccaggaaaca gcatcggcat
    17581 cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc cgtcagcagg cgaatttgat
    17641 acggcttttt ggcctcatgc tgttcgttag ctctgcgggc aatcgcggtg ggtagggctt
    17701 tcggcgaacc cgccggggta aagccgctaa atgccaccat gtcgttatgc tggataattt
    17761 ctgccgcttc atcggcggtc atccttgtcc actgagtttc catttttact ccttcgttcg
    17821 cagggattaa tgaccaacga aattaggttt acgtttttcg aggaaagcgt tcattccttc
    17881 ctggtaatct tcgctgtcat aaaccgcgcg tcgcatcccc tgaatacgtt caaattcatc
    17941 ggagttcatg gtgtgcgctt cgcccagtac acgcagctct tctttgataa cagcaatggc
    18001 taacggcgct ttctcagaga tgtggtgcgc catttgtaag gtgaaatctt ccagttcttc
    18061 cacttccaca acatggttga ggatgccgac agccaacgcg cgctgggcgg tgattggcga
    18121 agcggtaaaa atcagttctt tgacaatgtg gaagcccgcg tcgcgggtca ggttgtgaat
    18181 gccgaccagg ttatacggga cgccgaggtt tacaggcgtc attgagaagg ttgaggtact
    18241 ggcggcgata atcagatcgg aactcatgat catttcaaat gcgccacccc aaacactacc
    18301 ttccaccatc gaaatgatcg gtttcgggaa tttttggatc atgcgggtga tttgacgcaa
    18361 tggatcatca taggagagcg gatcgcgacc gccagacggc agttcgtgaa tatcgtggcc
    18421 tgcggagaag actttggatc cactcggcgc gcgcaaaatg atacagcgaa tttccggccg
    18481 gttgagatcg cttaatgcct gcataagatc atcaataaag actttactta aggcattaag
    18541 tttgcggcca tagttaaact caatgaccgc cactttgttg atagtgacaa cgttaacata
    18601 ctgataagac ataaaaattc ctttaatcaa aatattgcgt ctggataaat tcactgagct
    18661 gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac cgctaaaagc gtctggcggt
    18721 aatagcgatc gaaatcttca ttcgcgaaca ggtgattcag tacttcttct tcggtctgct
    18781 tacgcagcca ttccaccgat tgttgttgcc gcacttgttg taaacgacca ctggcagtta
    18841 gcgcggtttt gaagtcgatg atggcgtgcc agatctcatc gattccacgt ttttccagtg
    18901 cgctacaagt cagaacccgt ggctgccatt cgtcgtattt acgtcgcaga atatgcaggg
    18961 cactctcgta catatgccgg gcaatggcga cattggtatg gttatcgcca tcgtctttgt
    19021 tgataacgat cagatcagcc acttccatca gccctttttt aatgccctgc agatcatcgc
    19081 cgccaccggc aatttgcaac gagataaaac agtccaccat gcgggcgact tctgtttccg
    19141 actgcccgac gccaaccgtt tcgacaatca ctacgtcata acccgctgct tcgcataaca
    19201 gcattaattc ccgcgctcgc tgactggcac cgcccagatg accggaggat ggtaccgggc
    19261 gaataaacgc cgcttcggca cgcgccaggt cattcatgcg ggttttatcc ccgagaatgc
    19321 taccgccagt gaccgggctg ctgggatcga ccgcaataac cgcgaccttt aatccctctc
    19381 gaatcaacaa catgccaaag gcctcaagaa aggtactttt ccccgcgccg ggggtgccgg
    19441 taacgcccag tcgcagggtg ttaccgcagt acggcataat ggcatcaagc agctgcgtac
    19501 ttagtgcctg atgacgcggg tgacggcttt ccaccagcgt catggcctgg gcgagtgtgg
    19561 cacgctcacc ctgacgtaag cggcgaatac tttctgccag cgtggcttca ttaatcatga
    19621 tgctggctta tcagattcag tacgtcgcgc acactgtcga gcataggtgt acctggacca
    19681 taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat cctgcggcgg aatgacgcca
    19741 cccgcgacca cgcagatatc ttcgcgtccc cattttttca gcgcttcgac cagttccggg
    19801 atcagcgttt tatgaccggc agccagtgag gatgcgccca ctacgtgaac gtcgttttct
    19861 acggccaggc gggcgatctc ttcaggtgta gagaacatcg ggcttaaatc tacgtcgaaa
    19921 ccgagatcgg aataggcgct ggcgatcact ttcgcgccgc gatcgtgtcc atcctggccc
    19981 atcttagcga tcagaatgcg cgggcgacga ccattgtcgg caaggaactg ctccgtttgc
    20041 gcaacaatgg catcgaactc ggaggccgat ttctcagact gatgatagct ttgcgcaatc
    20101 acgccggtaa cacactggct tggcaccaga taacggtcga aagcgacttc cagcgcatcg
    20161 gaaatttcac ccagggtggc gcgaacgcga gcggcattaa cagcggcagc cagcaggttt
    20221 tcgttatgct gtgcggcgtg agtcagggcg ttcaacgcgg cggttacggc ggcatcatca
    20281 cgggtggcgc gaatgcgttc cagcgaagca atttgctcgt tacgcaccat cacgttgtcg
    20341 atctcaagta catcggtttc gtcttcgtga tccagtttgt acttgttgac accaacgatg
    20401 acacgcttgc cctggtcgat cagcgactgt tcgcgcgctg aggcctcttc gatcattcgt
    20461 tttggcagac ctgcttcgat cgctttcgcc atgccaccgg cttcgtcgat ctgttggata
    20521 atagctctgg cttgtttgac gatttgatcg gtcagcgact caatgtaata ggatccggcc
    20581 agtggatcga cggtgcggca gagttctgat tcttcctgga tgatgatctg ggtgttgcgg
    20641 gcaatgcgtg ctgagaaatc ggtaggcaaa ccaagcgctt cgtcaaaggc gttggtatgc
    20701 agtgactgag taccgcccag cgtcgcagcc agtgcttcaa tggtggtgcg gataacgttg
    20761 ttatacggat cctgttcagt caggctccag cctgaggtct ggcagtgggt acgcagcgcc
    20821 agtgatttcg gatcctgtgc gccaaatcca ctgaccgctt cgctccataa ataacgtgcc
    20881 gcacgcaaca tggcgacgtt cataaacaga tccatgccga taccgaagaa gaacgacagg
    20941 cgaggagcga agtcatcaat tttcagtccg gcagagattg ctgctttgat gtactcaatc
    21001 ccatcagcga gcgtaaatgc tacctgctgc acgcagttgg cacccgcttc ccccatgtgg
    21061 taaccgctga tactgatggt attaaatcgc ggcatgttgc cggaacacca ggcgatgatg
    21121 tcggcgataa tgcgcattga cggttttggt gggtaaatat aggtgttgcg gcagaggtac
    21181 tctttgagga tatcgttctg aatggtgccg gtcagtttat caggtgtaac accttgctct
    21241 tctgcggcga cgatataaaa cgccagtact ggtagcactg cgccattcat ggtcatcgaa
    21301 accgacattt tatccagcgg gatctggtcg aacaggactt tcatatcttc cacggtgtcg
    21361 atagcgacgc ccgctttgcc gacgtcgccc gccacgcgcg ggttatcgga gtcgtagcca
    21421 cggtgggtgg caaggtcaaa cgcaacggaa agaccttttt gcccggcggc caggttacgg
    21481 cgataaaaag cgttggactc ttttgctgtt gaaaaaccag catactgacg gatggtccac
    21541 ggttgggcgg tatacatagt ggcacgcggg ccacgaacgt agggcggcaa accaggaagg
    21601 gtacctgtca cctccagatt atcgagatcg gcttcggtat acagcggctt gatggcgatc
    21661 ccttccgcgg tttgctgaac cagcgagtcg acagttttct cccgacggct caattccttg
    21721 ttggcaagct gttgccactc ctgcacgtta gacattattt gctccgtaaa acgcaatcaa
    21781 aatgagggta tttgcgccta cggtgagctt tttacgtggg tttgtcgcca gcaaaaaaaa
    21841 tgcattcaga cgtcgttttt tgccgcctga gatttgtcta ttttgtgatg tgattcaagg
    21901 aatacaaaaa gaagtgagga caacgcctga tatgtgctat caggcattat ttgatggatt
    21961 aatcctgacg aaggactttg tgaccataat cgagtaacgc atcagtgact ttacgcatca
    22021 tgcggctttc aggagcaaag cggtgccagt agagcatccg tcgttgaaat agcccaggcg
    22081 ttaagtcaat cagttcaccg ctggccagct ctttctcgat ttgcaggtgc gggatcatac
    22141 agcaggtggt gccctggcga gcaagttgta cgaacgcttc tgaagaatta acgatatggc
    22201 agggcacgct gcctggaggc agatcgaagt tttgctgcaa aaaggcctgg tgcatatcgt
    22261 caagatggtc aaacgcgacc actggcgctt tcagtaatgc cgaacgcgtt acgccgttag
    22321 ggaaatattt ttcggcaaag ggttttgagc tgacgaacag atagtcgagc gcaccaagtt
    22381 tatcgacaag acaactcggc agcgcctgat gttgaatact caccgcgccg accacttcgc
    22441 cgcggcgcag acgttcctga gtgcgggttt catcttctac ctgcaagttg aggcggatag
    22501 gcgaatcagc caacacagga gccagtgcag gaagcaacca cgtcgccaga ctgtcggcgt
    22561 tgaccgccag tgaaagcagc agcggagtcg aaccggtttg ttcatcgccc agccactctt
    22621 cttccagcaa ctccacctgg cgcagcagtg ccagcagttt ttgcccttgt tccgtcgggc
    22681 gcggcggtac ggtacgcacc aacagcggct gcccgaacat attttccagt tgcttaatgc
    22741 gctgtgagac ggctgattgt gtaatgcaca gcttttgtgc cgcgcgctca aatcctcgtt
    22801 cacgtatcac cgcatccagt gcctgtaatg ttctgtagtc cgggcgtttc attgctgttg
    22861 tctgctcctg gtaaatgctt tttcctgcac tatgacacaa ttttgtgtca ggttgcatat
    22921 accgaagccg tcggcgggta acatttgtat tcgtggttaa acgcgcagct taaaagatca
    22981 gggatttgcg ctaatgcatt tcgctcagcg agttcgcgcc ctggttgtgt tgaatggcgt
    23041 ggcgttattg cctctgttcg caccgtggag cggcataccc agaccgttgt atgctttatt
    23101 tgcggggcga aaggggatgc ccgccattgc gcgatatttt atggatgagt taaccacgcg
    23161 gcttgccaac ggggtctgaa tcgctttttt tgtatataat gcgtgtgaaa tttcatacca
    23221 caggcgaaac gatcatgacg caggatgaat tgaaaaaagc agtaggatgg gcggcacttc
    23281 agtatgttca gcccggcacc attgttggtg taggtacagg ttccaccgcc gcacacttta
    23341 ttgacgcgct cggtacaatg aaaggccaga ttgaaggggc cgtttccagt tcagatgctt
    23401 ccactgaaaa actgaaaagc ctcggcattc acgtttttga tctcaacgaa gtcgacagcc
    23461 ttggcatcta cgttgatggc gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg
    23521 gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt tgcagaaaaa tttatctgta
    23581 ttgcagacgc ttccaagcag gttgatattc tgggtaaatt cccgctgcca gtagaagtta
    23641 tcccgatggc acgtagtgca gtggcgcgtc agctggtgaa actgggcggt cgtccggaat
    23701 accgtcaggg cgtggtgacc gataatggca acgtgatcct cgacgtccac ggcatggaaa
    23761 tccttgaccc gatagcgatg gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg
    23821 gcttgtttgc taaccgtggc gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa
    23881 ccattgtgaa atgatctgac gggggaacct cccccgttaa aaaaattctc ttcattaaat
    23941 ttggtgacat gtgtcacgct tttaccaggc aattgtcgat tgctctaaat aaatcctcta
    24001 aaccagcata ttcatccaag aattaccttt gcgtgatatt tcctcaacat cgcgacgcaa
    24061 acgttcatat tgccgcaata ttattttttg atatgttgaa aggcggatgc aaatccgcac
    24121 acaacatttc aaaagacagg attgggtaaa tggcaaaggt atcgctggag aaagacaaga
    24181 ttaagtttct gctggtagaa ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg
    24241 gttacaccaa catcgaattt cacaaaggcg cgctggatga tgaacaatta aaagaatcca
    24301 tccgcgatgc ccacttcatc ggcctgcgat cccgtaccca tctgactgaa gacgtgatca
    24361 acgccgcaga aaaactggtc gctattggct gtttctgtat cggaacaaac caggttgatc
    24421 tggatgcggc ggcaaagcgc gggatcccgg tatttaacgc accgttctca aatacgcgct
    24481 ctgttgcgga gctggtgatt ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca
    24541 atgctaaagc gcaccgtggc gtgtggaaca aactggcggc gggttctttt gaagcgcgcg
    24601 gcaaaaagct gggtatcatc ggctacggtc atattggtac gcaattgggc attctggctg
    24661 aatcgctggg aatgtatgtt tacttttatg atattgaaaa caaactgccg ctgggcaacg
    24721 ccactcaggt acagcatctt tctgacctgc tgaatatgag cgatgtggtg agtctgcatg
    24781 taccagagaa tccgtccacc aaaaatatga tgggcgcgaa agagatttcg ctaatgaagc
    24841 ccggctcgct gctgattaat gcctcgcgcg gtactgtggt ggatattccg gcgctgtgtg
    24901 atgcgctggc gagcaaacat ctggcggggg cggcaatcga cgtattcccg acggaaccgg
    24961 cgaccaatag cgatccattt acctctccac tgtgtgaatt cgacaacgtc cttctgacgc
    25021 cacacattgg cggttcgact caggaagcgc aggagaatat cggcctggaa gttgcgggta
    25081 aattgatcaa gtattctgac aatggctcaa cgctctctgc ggtgaacttc ccggaagtct
    25141 cgctgccact gcatggtggt cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc
    25201 taactgcgct gaacaaaatc ttcgccgagc agggcgtcaa catcgccgcg caatatctac
    25261 aaacttccgc ccagatgggt tatgtagtta ttgatattga agctgacgaa gacgttgccg
    25321 aaaaagcgct gcaggcaatg aaagctattc cgggtaccat tcgcgcccgt ctgctgtact
    25381 aattcccctt ctctgaaaat taacgggcag gtcactgact tgcccgtttt tttatccctt
    25441 ctccacaccg ttgccgcgtt gcgcattcta ctttgcgagt ctgctccaga aaataaatca
    25501 aagtcagatc aatgcgttgt gtttatttaa tggggattta cgatggcggg acagcatggg
    25561 gctgtaacgg taaagccctc accgaagcga gggcttaaag gagaagggtt atgatgcgac
    25621 ttgtcatcat actgattgta ctgttactga taagtttcgg agcttattaa cagtcagtct
    25681 caggggagga gtcatcctcc cttaccctta ctcactaaat taggtcaaag aatcaacgat
    25741 gtcaatcagg gcgatgcggg gtgcatcgcc ctgaaccgaa ctttttgata gccacttcat
    25801 cgcccacgtt tactgccgta atagatcctt tttcccaaaa attatctctg ttttgcatgg
    25861 ggtttttcga gcacgctcga agaaaagatt taaaggtaga tcaatgcgtt gtggttattt
    25921 aaagagagct tacgatagct gggcagcatg gggctgtaac ggtaaagccc tcaccgaagc
    25981 gagggcttga aggagaaggg ttatgatgcg acttgccatc atactgattg tactgttact
    26041 cataagtttc ccagcttatt aacagtcagt cacaggggag gagcaatcct cccttaccct
    26101 tactcactaa attaggttaa agaatcaacg atgtcaatca gggcgatgca ggtgtatcgc
    26161 ccttaccact cccagacttt cgacggtgta accaccgcag gaagagggat atcccactct
    26221 tcaacgggga gtttttccac caactgacaa tcatgcgcat aacccaccgg ttgcgtttta
    26281 tagtgctgcc agttttgtaa ggtccgatca taaaaaccac cgcccattcc caggcgctga
    26341 ccgtactcat caaaggcgac cagcggtgtg atcagcacgt ctaatcggga aaggggtagc
    26401 acgtcacgca catccagttt tggctcatgg atcttcaacc tgttcatcac cagttcgctt
    26461 tgcggatggt aattcaggaa cagcaaatta ccggcactaa agggatgcaa aactggaaga
    26521 tatacgcgct taccggcgcg ccagagttgt tctattagcg gctgggtgtc gagttcacca
    26581 tcaaaagaga ggaatacagc gaccgtatgc gccatcacca ccgggggata agtcatcatc
    26641 cgggtagcgg cttgttgacc catttcctgc tgttgttccg gcgttaacgc acgacgacgt
    26701 tgccgaatca ttttgcggat ttcttgtcgg gataatgtca gtgggagttc tggtagttgc
    26761 gtcatggctg gtgccagata agaagggaat ctccgagatg ccgccgcagg ctgtaaccct
    26821 tgaacccttg gttcaaggtg aatgtgtcgt cgcagtttta aggcttctcg gacggaccga
    26881 gcatgctcac caaccgcgga gcgccacatt cttgtggtat gaaatatcgg ctcaggggac
    26941 tggcccgctt gcgaacatct cagagaaatt ttgtcttcac ggttactcta ccacagtaaa
    27001 ccgaaaagtg tcattcaaag ttttggttag ttttttcggt gatgcgacct tgttcaagta
    27061 acgcttgttc tatggtctgc tgcagcatcc gaatacgctg ttccatactt gccgcgtagt
    27121 cacgagtctt cgctttttct tgcgctaact catagctgat attcaatgcg gcaatgaaga
    27181 ccaactgttc agtatttgtg actctagtgc gttctttcag atcttgcaac cgttggttca
    27241 gatcgtccgc tgcctgattc aacgcatccc tttggtcagg cgggcagttc acacgcagtg
    27301 aacggccaaa aatttggata tcgacgggtt gtgcagacat gccaccttcc tgctgattga
    27361 ctgcgctgcc ttcgtcttta gacccatggt ctgcgaaggg gcgacactat agctaccctg
    27421 atgagaagag acaagccctt ttctggtcca ccagggccca aagtggtagc atatcatgaa
    27481 tattcctccc tttgacgacg aatgcttatg tctatacaga acgaaatgcc tggttacaac
    27541 gaaatgaacc agtatctgaa ccaacaaggg acgggtctga cccctgctga gatgcatggt
    27601 ttaatcagtg ggatgatatg tggcggtaac gatgacagct catggctacc gctacttcac
    27661 gacctgacga acgaaggcat ggctttcggt catgagctgg cacaggcact gcgtaaaatg
    27721 cactctgcca ccagcgatgc cctgcaggat gacggcttcc tttttcagct ttatctgcct
    27781 gatggcgatg atgtcagcgt tttcgatcgg gctgatgcat tggcaggttg ggtcaatcac
    27841 ttcctgcttg gtcttggcgt tacgcaaccg aagctggata aagtgaccgg cgaaaccggt
    27901 gaagctatcg acgatctgcg taacattgcg caactgggtt acgacgaaga cgaagatcag
    27961 gaagagcttg aaatgtcgct tgaagagatc atcgaatacg ttcgtgttgc cgcgctgtta
    28021 tgccacgaca cctttactca tccgcaaccg accgcgccag aagtacaaaa accgactcta
    28081 cactaaaaac aaaaaacgta aggagagtgt tatgagtgag atatcccggc aagagtttca
    28141 gcgtcgccgt caggccctgg tggagcaaat gcaacccggc agcgccgcgc tgatttttgc
    28201 tgcaccagaa gtaacacgta gcgccgacag cgaatacccc tatcgtcaga acagtgactt
    28261 ctggtacttc accggcttta acgaaccgga agcggtgctg gtgctgatta aaagcgatga
    28321 cactcataac cacagcgttc tgtttaaccg cgttcgcgac ctgacggcgg agatctggtt
    28381 tggccgtcgc ttaggccagg atgccgcgcc agagaaactg ggcgttgacc gcgcactggc
    28441 attcagcgaa atcaatcagc aactttatca actacttaac ggcctggatg tggtttacca
    28501 tgcccagggc gaatatgcat atgctgatga aatcgtgaac agtgcgctgg aaaaactgcg
    28561 taaaggttcg cggcaaaatc tcaccgcacc ggcaacgatg atcgactggc gtcctgttgt
    28621 tcatgaaatg cgcctgttca aatcgccaga agagattgcc gtactccgcc gcgcgggaga
    28681 aatcaccgcc atggcacata cccgggcgat ggaaaaatgc cgtccgggaa tgttcgagta
    28741 ccatctggaa ggcgaaattc accacgaatt taaccgccac ggtgcgcgct atccgtccta
    28801 taacaccatt gtcggcagcg gtgaaaacgg ctgcattctg cactacaccg aaaacgagtg
    28861 tgaaatgcgc gacggcgacc tggtgttgat tgacgcgggt tgcgaataca aaggttacgc
    28921 tggcgatatt acccgcacct tcccggtcaa cggcaaattc actcaggccc agcgtgaaat
    28981 ctacgacatt gtgctggagt ctctcgaaac cagcttgcgc ctgtatcgtc cgggaacttc
    29041 cattctggaa gtcactggtg aagtggtgcg catcatggtt agcggcctgg taaaactcgg
    29101 cattctgaaa ggtgatgttg atgaactgat cgctcagaac gcccatcgtc ctttctttat
    29161 gcatggcctt agccactggt tagggctaga tgtccatgac gttggcgttt atggtcagga
    29221 tcgctcgcgc attctggaac cggggatggt actgaccgta gagcctggtc tgtatatcgc
    29281 gccggatgcg gatgtgccag aacaatatcg cggtatcggc attcgtattg aagacgacat
    29341 tgtgattacc gaaaccggca acgaaaacct caccgccagc gtggtgaaaa agccggaaga
    29401 aatcgaagcg ttgatggcag ctgcgagaaa gcaatgagcg taatcatcgt cggtggcggc
    29461 atggcgggcg cgacgctggc gctggctatt tcccggttaa gtcacggggc gctgccggta
    29521 catttgattg aagcgaccgc gccagagtca catgctcatc cgggctttga tggacgagct
    29581 attgcgctgg ctgcggggac ctgtcagcaa ctggcgcgca tcggcgtctg gcaatctctg
    29641 gcggattacg caaccgccat cactaccgtg catgtcagcg atcgtggtca cgctggattt
    29701 gtcaccctcg ccgcagaaga ttaccaactg gcggcgctgg gacaggttgt cgaattgcac
    29761 aatgtcgggc aacggctgtt tgcattgctg cgtaaagcac ctggcgtaac gctgcattgc
    29821 cctgatcgcg tggctaacgt tgcccgtact cagagtcacg ttgaagtgac gctggagagt
    29881 ggcgagacgc tgacgggccg cgtgctggta gcagctgatg gcacccattc agcgttagcc
    29941 accgcgtgcg gcgttgactg gcagcaggag ccttacgaac aactggccgt gattgccaac
    30001 gttgcgactt ccgttgcgca cgaaggccgc gcttttgaac gcttcacgca acatggcccg
    30061 ctggcgatgt tgccgatgtc tgacggacgc tgttcgctgg tctggtgtca tccactggaa
    30121 cggcgcgaag aggtgctgtc gtggagcgac gagaagtttt gccgtgaact ccagtcggcc
    30181 tttggctggc gacttggaaa aattacccac gccggtaaac gcagtgctta tccgctggcg
    30241 ttaacccgcg ccgccagagc tattacccat cgtaccgtgc tggtgggcaa tgcggcgcaa
    30301 actctgcacc cgattgccgg gcaagggttt aacctcggta tgcgagatgt catgagcctt
    30361 gcggaaaccc tgactcaggc gcatgagcgc ggagaagaca tcggtgatta cggcatattg
    30421 tgccgctatc agcagcgtcg acagagtgat cgcgaagcaa ccatcggcgt cacggacagc
    30481 ctggtacatc tttttgccaa ccgttgggca ccgctggttg tcgggcgcaa catcggactg
    30541 atgacgatgg aattattcac cccggcacgc gatgtgctgg cgcagcgcac cctcggttgg
    30601 gtggcgcgtt gaaggcggtt ttaaggaata aacaatgcaa agtgttgatg tagccattgt
    30661 tggtggcggc atggtggggc tggcggttgc ctgcggctta caggggagcg gcttacgcgt
    30721 tgccgtactg gagcagcgcg taccggaacc tctggcggcg gatgcaccac cacaactgcg
    30781 cgtttcggct atcaatgccg ccagcgaaaa attactcacc cgtcttggcg tctggcagga
    30841 cattctctct cgtagggcca gttgttatca cggtatggaa gtgtgggaca aagacagttt
    30901 tggtcacatt tcgtttgacg atcaaagcat gggctatagc catcttgggc atatcgttga
    30961 aaactcagtg attcactacg cgctgtggaa caaagcgcag cagtcgtcag atatcactct
    31021 gttggccccc gcagaattac agcaggtcgc ctggggagaa aatgaaactt tcctgacgct
    31081 gaaagatggc agcatgttaa cggcgcgtct ggtgattggc gcggacggtg ctaattcctg
    31141 gttgcgcaac aaagcagata ttccgctgac tttctgggat tatcagcatc acgcgctggt
    31201 agcgaccatt cgcacggaag aaccgcatga tgcggtggcg cggcaggttt tccatggcga
    31261 aggcattctg gcctttttac cgcttagcga tccgcatctt tgctcaattg tctggtcact
    31321 gtcgccagag gaagcgcagc ggatgcagca ggcaagtgaa gatgaattta atcgcgcgtt
    31381 aaatatcgct tttgataatc gcctgggctt atgcaaggtt gagagcgcgc gtcaggtgtt
    31441 cccactgacg gggcgttatg cgcgccagtt tgccgcgcac cgtctggtgc tggtgggcga
    31501 cgccgcgcat accattcacc cgctggcggg gcagggagta aatctcggct ttatggatgc
    31561 tgcagagctg attgctgaac tgaaacggtt gcatcgtcag gggaaagaca tcgggcagta
    31621 catttacctg cgccggtatg agcgtagccg caagcacagt gcggcgctga tgctggctgg
    31681 tatgcaggga ttccgcgatc tgttttccgg taccaatccg gcgaaaaaac tgctgcgtga
    31741 tattggtttg aaactggccg acacgcttcc tggcgttaaa ccacaactta tccgccaggc
    31801 aatgggatta aacgatttgc ctgaatggct gcgttaaaaa tttctcttct gctgtttatt
    31861 tgatacccat cacactttca tctcccggtt ttttcgccgg gagattttcc tcatttgaaa
    31921 taaactaatt tcacctccgt tttcgcatta tattttctaa tgtcacgaat ttttgattta
    31981 gtgttttttg acattttttc agctcttaat attgccttat tcaaattaac tttctcatca
    32041 catcatcttt atataaaaac ttgtgtattt tttggttttt tattctgttg cgattttggc
    32101 gttttttaac cataagctaa tgtgatgatc aattttacct tatggttaac agtctgtttc
    32161 ggtggtaagt tcaggcaaaa gagaacgatt gcgttgggga ccgggagtgg ctccgatgct
    32221 gggtttcgtg gtgataattt caccatgaaa aagttgtcag ccccgcttat tcaatgagga
    32281 caagatggca caacagactc ctttgtacga acaacacacg ctttgcggcg ctcgcatggt
    32341 ggatttccac ggctggatga tgccgctgca ttacggttcg caaatcgacg aacatcatgc
    32401 ggtacgtacc gatgccggaa tgtttgatgt gtcacatatg accatcgtcg atcttcgcgg
    32461 cagccgcacc cgggagtttc tgcgttatct gctggcgaac gatgtggcga agctcaccaa
    32521 aagcggcaaa gccctttact cggggatgtt gaatgcctct ggcggtgtga tagatgacct
    32581 catcgtctac tactttactg aagatttctt ccgcctcgtt gttaactccg ccacccgcga
    32641 aaaagacctc tcctggatta cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg
    32701 tgatgacctt tccatgatcg ccgtacaagg gccgaatgcg caggcaaaag ctgccacact
    32761 gtttaatgat gctcagcgtc aggcggtgga agggatgaaa ccgttctttg gcgtgcaggc
    32821 gggcgatctg tttattgcca ccactggtta taccggtgaa gcgggctatg aaattgcgct
    32881 gcccaatgaa aaagcggccg atttctggcg tgcgctggtg gaagcgggcg ttaagccatg
    32941 cggtctgggc gcgcgtgaca cgctgcgtct ggaagcgggc atgaatcttt atggtcagga
    33001 gatggacgaa actatttctc ctttagccgc caacatgggc tggactatcg cctgggaacc
    33061 ggcagatcgt gacttcatcg gtcgtgaagc cctggaagtg cagcgtgagc atggcacaga
    33121 aaaactggtt ggtctggtga tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg
    33181 ttttaccgat gcgcagggca accagcatga aggcattatc accagcggga ctttctcccc
    33241 gacgctgggt tacagcattg cgctggcacg cgtgccggaa ggtattggcg aaacggcgat
    33301 tgtgcaaatt cgcaaccgtg aaatgccggt taaagtgacg aaacctgttt ttgtgcgtaa
    33361 cggtaaagcc gtcgcgtgat ttactttttt ggagattgat tgatgagcaa cgtaccagca
    33421 gaactgaaat acagcaaaga acacgaatgg ctgcgtaaag aagccgacgg cacttacacc
    33481 gttggtataa ccgaacatgc ccaggagctg ttaggcgata tggtgtttgt tgacctgccg
    33541 gaagtgggcg caacggttag cgcgggcgat gactgcgcgg ttgccgaatc agtaaaagcg
    33601 gcgtcagaca tttatgcgcc agtaagcggt gaaatcgtgg cggtaaacga cgcactgagc
    33661 gattccccgg aactggtgaa cagcgaaccg tatgcaggtg gctggatctt taaaatcaaa
    33721 gccagcgatg aaagcgaact ggaatcactg ctggatgcga ccgcatacga agcattgtta
    33781 gaagacgagt aacggcttta ttcctcttct gcgggagagg atcagggtga ggaaaattta
    33841 tgcctcaccc tcactctctt cgtaaggaga gaggttcaca attcactgca cgtttcagga
    33901 accatcgctc atgacacaga cgttaagcca gcttgaaaac agcggcgctt ttattgaacg
    33961 ccatatcgga ccggacgccg cgcaacagca agaaatgctg aatgccgttg gtgcacaatc
    34021 gttaaacgcg ctgaccggcc agattgtgcc gaaagatatt cagcttgcga ccccgccgca
    34081 ggttggcgca ccggcgaccg aatacgccgc actggcagaa ctcaaggcta ttgccagtcg
    34141 caataaacgc ttcacgtctt acatcggcat gggttacacc gccgtgcagc taccgccggt
    34201 tatcctgcgt aacatgctgg aaaatccggg ctggtatacc gcgtacactc cgtatcaacc
    34261 tgaagtctcc cagggccgcc ttgaagcact gctcaacttc cagcaggtaa cgctggattt
    34321 gactggactg gatatggcct ctgcttctct tctggacgag gccaccgctg ccgccgaagc
    34381 aatggcgatg gcgaaacgcg tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc
    34441 cgatgtgcat ccgcaaacgc tggatgtggt ccgtactcgt gccgaaacct ttggttttga
    34501 agtgattgtc gatgacgcgc aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt
    34561 acagcaggtt ggcacaaccg gtgaaatcca cgactacacc gcgcttatta gcgaactgaa
    34621 atcacgcaaa attgtggtca gtgtcgccgc cgatattatg gcgctggtgc tgttaactgc
    34681 gccgggtaaa cagggcgcgg atattgtttt tggttcggcg caacgcttcg gcgtgccgat
    34741 gggctacggt ggcccacacg cggccttctt tgcggcgaaa gatgaataca aacgctcaat
    34801 gccgggccgt attatcggtg tatcgaaaga tgcagctggc aataccgcgc tgcgcatggc
    34861 gatgcagact cgcgagcaac atattcgccg tgagaaagcg aactccaaca tttgtacttc
    34921 ccaggtgctg ctggcaaaca tcgccagcct gtatgccgtt tatcacggcc cgattggcct
    34981 gaaacgtatc gctaaccgca ttcaccgtct gaccgatatc ctggcggcgg gcctgcaaca
    35041 aaaaggtctg aaactgcgcc atgcgcacta tttcgacacc ttgtgtgtgg aagtggccga
    35101 caaagcgggc gtactgacgc gtgccgaagc ggctgaaatc aacctgcgta gcgatattct
    35161 gaacgcggtt gggatcaccc ttgatgaaac aaccacgcgt gaaaacgtaa tgcagctttt
    35221 cagcgtacta ctgggcgata accacggcct ggacatcgac acgctggaca aagacgtggc
    35281 tcacgacagc cgctctatcc agcctgcgat gctgcgcgac gacgaaatcc tcacccatcc
    35341 ggtgtttaat cgctaccaca gcgaaaccga aatgatgcgc tatatgcact cgctggagcg
    35401 taaagatctg gcgctgaatc aggcgatgat cccgctgggt tcctgcacca tgaaactgaa
    35461 cgccgccgcc gagatgatcc caatcacctg gccggaattt gccgaactgc acccgttttg
    35521 tccgccggaa caggctgaag gttatcagca gatgattgcg cagctggctg actggctggt
    35581 gaaactgacc ggttacgacg ccgtttgtat gcagccgaac tccggcgcac agggcgaata
    35641 cgcgggcctg ctggcgattc gtcattatca tgaaagccgc aacgaagggc atcgcgatat
    35701 ctgcctgatc ccggcttctg cgcacggaac taaccccgct tctgcacata tggcaggaat
    35761 gcaggtggtg gttgtggcgt gtgataaaaa cggcaacatc gatctgactg atctgcgcgc
    35821 gaaagcggaa caggcgggcg ataacctctc ctgtatcatg gtgacttatc cttctaccca
    35881 cggcgtgtat gaagaaacga tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca
    35941 ggtttacctt gatggcgcga acatgaacgc ccaggttggc atcacctcgc cgggctttat
    36001 tggcgcggac gtttcgcacc tcaacctgca taaaactttc tgcattccgc acggcggtgg
    36061 tggtccgggt atgggaccga tcggtgtgaa agcgcatctg gcaccgtttg taccgggtca
    36121 tagcgtggtg caaatcgaag gcatgttaac ccgtcagggc gcagtttctg cggcaccgtt
    36181 cggtagcgcc tctatcctgc caatcagctg gatgtacatc cgcatgatgg gcgcagaagg
    36241 gctgaaaaaa gcaagccagg tggcaatcct caacgccaac tatattgcca gccgcctgca
    36301 ggatgccttc ccggtgctgt ataccggtcg cgacggtcgc gtggcgcacg aatgtattct
    36361 cgatattcgc ccgctgaaag aagaaaccgg catcagcgag ctggatattg ccaagcgcct
    36421 gatcgactac ggtttccacg cgccgaccat gtcgttcccg gtggcgggta cgctgatggt
    36481 tgaaccgact gaatctgaaa gcaaagtgga actggatcgc tttatcgacg cgatgctggc
    36541 tatccgcgca gaaatcgacc aggttaaagc cggtgtctgg ccgctggaag ataacccgct
    36601 ggtgaacgcg ccgcacattc agaacgaact ggtcgccgag tgggcgcatc cgtacagccg
    36661 tgaggttgca gtattcccgg caggtgtggc agacaaatac tggccgacgg tgaaacgtct
    36721 ggatgatgtt tacggcgacc gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca
    36781 gtaattcact gattcgacta ttttctaaag gcgcttcggc gcctttttag tcagatgaca
    36841 aagtacaaaa gtgctcagac agtcccctcg cccctttggg gagagggtga ggggaacagg
    36901 ccggcactgg cgcgaatatt taccctcacc ccggccctct ccctgaaagg gcgaggggga
    36961 aaagcgtgcc aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg
    37021 aatgaatttt tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc
    37081 atcgggcggg caactgcatt actgttggcg caagaagggt atacggtggc ggttaattat
    37141 cagcaaaacc tccacgcggc gcaggaagtg atgaacttaa taacgcaagc cggtggcaaa
    37201 gcattcgtgc tccaggcgga tatcagcgac gaaaaccagg tcgttgcgat gtttacagca
    37261 atcgatcagc acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc
    37321 cagtgcaccg ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg
    37381 acgggatatt ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc
    37441 agtggcggcg ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa
    37501 tatgttgatt acgcggcatc gaaaggggcg attgatacgt taaccaccgg actatcgctg
    37561 gaagtcgccg cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa
    37621 atgcacgcca gcggcggcga gcctggacgc gtcgatcgcg ttaagtcgaa catccccatg
    37681 cagcgtggtg gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa
    37741 gcctcttacg tcacgggaag ttttatcgat ttggcgggcg ggaaataaaa cagggaagtt
    37801 gtctgaccgg atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg
    37861 ttgctggcaa tcacctcttg gtaccagtta aagctcttct tacgtgaacg cgacatatcg
    37921 ccagcaccgt cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg
    37981 gtggtgaacg atacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacgcca
    38041 tcgtaagtca ccgctttttt catctcttcg atatgggcgc gcaggtagtc aatgcggtag
    38101 tcgtcgttga tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca
    38161 ataaacagcg gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact
    38221 ggatcaatct gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttcg
    38281 aaaccagaga tcgcatcgcc ggtgccgcct tcggccttca ctgcgttggt catgtaatag
    38341 ctgaaaccaa gataatcgca ggtgccttca cgcagtacat cgagatcgcc gtcttccatt
    38401 ttgatgttaa atccgcgacg ctcccactcg ttcaacacat aggacgggta atagccgcgt
    38461 agctgcacat cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg
    38521 tccgggttac agcagtaggg atagagcggc accatcgcca gcatacagcc gactttcatc
    38581 tccgggtgaa tgcgacgcgc agctttcacc gccagggcgc tggcgacaaa ctggtgatgc
    38641 agcacctgat acatcgtctc ttccgggttc tcatgctcgg tataaaccac gccggagcag
    38701 cagtaaccga acagcggtgc acgccagtta cgctggttgt taatttcgtt gaaggtcatc
    38761 cagtatttga ctttatgctt atagcgttca aacaccactt ccgcgaaacg gacaaagaaa
    38821 tcaaccactt tacggttggt ccagctaccg tattgctgca ccagatgcag cggcatttca
    38881 aagtgggaga gggtgatcac cggttcgatg ttgtatttca gcagttcatc gaacatatca
    38941 tcgtagaact tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc
    39001 gtccaggcga tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct
    39061 tccttgtagt gaccataaaa atcaacggct tcatggtttg gatagtattt gcctggcaag
    39121 acttctttgg tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg
    39181 ctcggccctt ttccgccttt gttccagccg ccttcgacct gatgagcggc aactgcgccg
    39241 ccccataaga aatctttcgg taaggtgagt tttttcacta tcataggctc cagatgagtc
    39301 aattcttaca ggaaatagta ccatgcagcc aaacgacatc actttttttc aacgtttcca
    39361 ggatgacatt ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt
    39421 caaaacgggt gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat
    39481 tgaagtcacc gcaacctcaa ccgtaacgct ggatacgctg actgaaaaac atgcagagca
    39541 ggaaaatatg accctgactg aactgataaa ggtcattgcc gacatctatc ccggtcagac
    39601 acagttttat gtgattgaat ttaaatgtct ttaaattgaa ctcgcgcctg ttagttgaaa
    39661 tttcgcattt aatttcatga tttttaggag attagtctca aatcagaatt tattttagct
    39721 aacaggtgtt cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg
    39781 tttctccgga gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag
    39841 aggaaattgc caacagcgtc agtcacggca ttgggttggt gtttggtatc gttgggctgg
    39901 tgttgctact ggttcaggcg gtggatctca atgccagcgc cactgcgata accagttaca
    39961 gcctctatgg cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc
    40021 ctcatcaacg ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga
    40081 ttgccggaac ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt
    40141 tgatgattgt tatctggagc ctggcattgc tgggtattct gtttaaactg accatcgcgc
    40201 accgattcaa aattttatct ctggtgacct atctggcgat gggctggctg tcgctggtgg
    40261 taatttatga aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg
    40321 gcgtggttta ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg
    40381 ccatctggca cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt
    40441 atattgggca ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc cagcgtcgca
    40501 tcaggcatcg gagcacaact gccggattcg gcgtgaacgc cttatccgat ctacgggcct
    40561 cgtccctgtt gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat
    40621 caggcgataa atgcctgatg cgataaagag atttactctt cgagcgaata cggcagcggc
    40681 tcgatatgca atgtattcgc atcgtcgcgt acgcggaaga tgctatccgg ttccatatcg
    40741 ttattcatga cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc
    40801 gtaccggtac gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc
    40861 agtcggctgg cgctacctgc cagcaaccag agcgcacgtt tattggcacc acggaatttt
    40921 gctcgcgcca ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc
    40981 gcctggagat tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg
    41041 aaacccgctt caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg
    41101 cgcagtttat cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct
    41161 gccgggtgtt caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc
    41221 gaaggcagtt cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca
    41281 agcagcacac gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc
    41341 agttcagtca gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg
    41401 tcgcggaaca gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc
    41461 aggtgctgat tttctgtcat ctggctgaca tctgctgtca cctgaccctg catatatttt
    41521 tcgctgtccg cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc
    41581 ggtaaacggg cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag
    41641 tcctgagttg cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa
    41701 gtgctttcac tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa tgaagccatt
    41761 gaattgttgt gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa
    41821 ggcgaaaaac acgttacaat agcggggtac tggattttcg tgagacagga agaagatgga
    41881 cattaacaac aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat
    41941 ttcaatcatg ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat
    42001 ctttattcgt ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg
    42061 taaaccggcc gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga
    42121 acgtggtcct gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc
    42181 tgattcatgg gctggttgcc gctgttattt tactcatgcc ctggccactc agttacaccc
    42241 cgttatggat ggtgttactt tcgctggtgg tgtttgattg cgttcgcagc cagcggcgta
    42301 ttaatgctcg ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc
    42361 aggagtggag catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac
    42421 gttctgatgg cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagctg
    42481 aatggcggga tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc
    42541 aaagcatatg cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg
    42601 atttgctcgc accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc
    42661 agcgccagac ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca
    42721 tatccttccg ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc
    42781 agcataccga gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac
    42841 agcgcaacaa ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag
    42901 tcttcctgga tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc
    42961 tccattaatt tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata
    43021 atatcgcgga ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga
    43081 cccatattca taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga
    43141 atcgctttag tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc
    43201 aagtgaaaca ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa
    43261 atctggctga aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt
    43321 tgcatcaccg cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg
    43381 cagaacggct ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc
    43441 gccgattgtt ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc
    43501 tcgcttcacc gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac
    43561 gtttattaca ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg
    43621 aagtgttgta tgctaccggg ctgcgtgtct ctgaactggt cggactgaca atgagtgata
    43681 tcagcctgcg tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtac
    43741 cgttaggtga agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc
    43801 tgctgaatgg tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc
    43861 gacagacctt ctggcaccgc attaaacatt atgctgtgct ggcgggtatc gacagcgaaa
    43921 agctgtcacc gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg
    43981 atttacgcgt ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata
    44041 cgcatgtcgc taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat
    44101 gtctgaaaag aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg
    44161 cgttttcagg ttttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg
    44221 gcatcaaaag cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta
    44281 acagcggcgt gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg
    44341 acgttagcgg cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg
    44401 cgcttgaaaa agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt
    44461 ttactgatat tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg
    44521 cgctggggat caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag
    44581 agaaagaaat gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga
    44641 tggcaggtaa aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac
    44701 ttggcgtcca gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg
    44761 ttccgggtta ccagccgccg aaagagatga aagaattcct cgacgaacac caaaaaatga
    44821 ccagcggtaa ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga
    44881 aacggcagac ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg
    44941 cgtgcgcagt gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact
    45001 gagcggcgtc gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat
    45061 tattgtggtc ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc
    45121 gatgcgctcg cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg
    45181 ttacggctta agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt
    45241 cacggtggat aacggtattt cctcccatgc gggggttgta cacgctcgct cgttgggcat
    45301 tccggttatt gttaccgatc accatttgcc aggcgaaaca ttacccgcgg cggaagcgat
    45361 cattaaccct aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt
    45421 ggcgttttat ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga
    45481 gcgtggcatc gcaattccta acctggcaga actgctggat ctggtcgcgc tgggaacagt
    45541 ggcggacgtc gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg
    45601 catccgtgcc ggaaagtgcc gtccggggat taaagcgctg ctggaagtgg caaatcgcga
    45661 tgcgcaaaaa ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc
    45721 cgggcgactg gacgatatgt ccgtcggtgt ggcgctgttg ctgtgcgaca acatcggcga
    45781 agcgcgcgtg ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca
    45841 aggaatgcaa gttgaagccc tgaccctgtg cgagaaactg gagcgaagtc gcgacacgct
    45901 acccggcggg ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc
    45961 ttcgcgcatc aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg
    46021 tacgctgaaa ggttcaggtc gctccattca ggggctgcat atgcgtgatg cactggagcg
    46081 attagacaca ctctaccctg gcatgatact gaagtttggc ggtcatgcga tggcggcggg
    46141 tttgtcgctg gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac
    46201 agagtggctg gacccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc
    46261 ggccgaaatg accatggaag tggcgcagtt gctgcgcgat gctggcccgt gggggcagat
    46321 gttcccggag ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga
    46381 acgtcatttg aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc
    46441 ttttaatgtc gataccgccc tctggccgga taacggcgtg cgtgaagtgc aactggctta
    46501 taagctcgat atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat
    46561 ctggccaatt tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct
    46621 cttatcaccg cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc
    46681 ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg
    46741 actacgacgc caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg
    46801 tctggaacga acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg
    46861 ttgtcgacac cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac
    46921 tggctgtaga agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc
    46981 tggaagaaaa actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg
    47041 ccgactgcta cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga
    47101 gcatgcttga gcgtatgtat ctgcgctggg ctgaatcgcg tggtttcaaa actgaaatca
    47161 tcgaagagtc ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg
    47221 attacgctta cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc
    47281 cgtttgactc cggcggtcgt cgccacacat cgttcagctc tgcgtttgtt tacccggaag
    47341 ttgatgatga tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg
    47401 cgtccggcgc gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca
    47461 tcccaaccgg gatcgtgacc caatgccaga acgaccgttc ccagcacaag aacaaagacc
    47521 aggccatgaa gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg
    47581 agaaacaggc gatggaagat aacaaatccg acatcggctg gggcagccag attcgttctt
    47641 atgtccttga tgactcccgc attaaagatc tgcgtaccgg ggtagaaacc cgcaacacgc
    47701 aggccgtgct ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat
    47761 gaggaaccaa catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca
    47821 atgaactgaa aacgcgtcgt gagaagctgg cgaacctgcg cgagcagggg gttgccttcc
    47881 cgaacgattt ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag
    47941 agaacgaaga actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc
    48001 gccgtattat gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc
    48061 tgtacgttgc ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg
    48121 acctcggcga catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt
    48181 ctatccactg caccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat
    48241 tccacggctt gcaggatcag gaagcgcgct atcgtcagcg ttatttggat ctcatctcta
    48301 acgatgaatc ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt
    48361 tcatggtgaa ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg
    48421 gtgccgctgc gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc
    48481 gtatcgcgcc ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg
    48541 aaatcaaccg taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca
    48601 tgatggaact ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc
    48661 tgttccgtac tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga
    48721 cgctggactt cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc
    48781 gcccggaaac cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat
    48841 ctatcggtat ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg
    48901 aagaagtggc agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag
    48961 tttctccgct ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct
    49021 tcatcggtgg tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg
    49081 cgcaacgctt cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct
    49141 acgatgaaga ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga
    49201 ttggcatcga ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc
    49261 tgttcccggc gatgcgtccg gtaaaataag cattacgtta tgctcacaac cccggcaaat
    49321 gtcggggttt tttaattgtg ctgcgcgaaa gcagacaaca attttcttgc ttcactattg
    49381 gctgcctgca tcaccatcca cggactgaac gcccacggtg tggcatcaat accgtgtaat
    49441 acatctgcta aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca
    49501 ctggtggtgc gtgcggcaaa taccggacac acttcatttt ccacaatgcc attcggatcg
    49561 gtggcgcgat agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc
    49621 ccataacggc aacggcggat caccgcgtct tcgttgcttt ctcccagttg tgggtgccca
    49681 caaaccgagt tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact
    49741 aataattgtc ctttggcatt aaacagccaa ctggagaacg cgcgatgtaa gcgggtgtct
    49801 gccgtgtgtg cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa
    49861 atgacgtgtt ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat
    49921 aattgaagag tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg
    49981 gatgccgaca ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa
    50041 tggccattgc aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac
    50101 tgaatatagc gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg
    50161 cgcatcagaa gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc
    50221 ttcaccaggt cagcatgatg ctgatagcca ttaaagaaga cattcagcac aacggcgcta
    50281 agggttgcga gcataatgcc gctatgcagc aacggttgca gtacggccgg taacttagaa
    50341 aagaaatcgt gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag
    50401 aggttgtaac ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt
    50461 accatgccga acatcaccag cccagcgccg cccagcacaa attgcggaat ggaggctacc
    50521 agcaccgcca tttttggcac catgccaaac aggattaata taattcccga agaaatacac
    50581 acccagcggc tatgaacgcg cgtcacgcta accaggccaa cgttttgaga aaatgacgtg
    50641 tgggggaagc tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg
    50701 cgaataatat cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc
    50761 cccatcgact cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga
    50821 tcgaaaatcg gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt
    50881 agcccggata aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc
    50941 agtaatacgg cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa
    51001 attaagacgg caaaggagat acctaaataa acgggattac catattgcgg attcccttta
    51061 cctccggcgg cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc
    51121 acaccggtaa ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat
    51181 gtggtgataa aacccgcggc tatagtggca ccaaatatcc ccagcaggcc gatatccggg
    51241 ttcatcccaa tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc
    51301 aggcggatcc ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag
    51361 caaaagagat ccgagctaat gagcatcgca atagcttctt ttgagaggcc cagtcggtca
    51421 ccaatcataa gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg
    51481 agtatgatca gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc
    51541 ccagaagatg agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc
    51601 cagagtaaaa agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc
    51661 cagtttttgc gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg
    51721 gttaaaccac gaaaatgaga agagaacctg acaatgaaat tatctgtaat tttctcatat
    51781 tgatatttca ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga
    51841 tagcgctctt ttggtcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata
    51901 atttgaactc agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt
    51961 tattgcatcg cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata
    52021 agtttatcgc tgctgaagct gcggaatgta taggctgtca tgcttgtgaa attgcctgtg
    52081 cggtggcaca caatcaagaa aactggccgc tgagtcacag tgactttcga ccgcgtatcc
    52141 acgttgtagg gaaaggccag gctgcgaatc cggtggcctg ccatcactgc aacaatgccc
    52201 cttgcgttac ggcttgtccg gttaatgctc tgactttcca gtccgatagc gtacaactgg
    52261 acgagcaaaa atgtattggc tgtaaaagat gcgcaatcgc ttgccccttt ggcgtcgttg
    52321 agatggtcga tacgattgcg cagaaatgcg acctttgtaa ccagcgcagt tccggcacgc
    52381 aagcctgtat tgaagtctgc ccaacacagg cgttacgact gatggacgat aaagggttac
    52441 agtggataaa ggtggctcgc cagcgcaaaa cggcagcagg aaaagcgtca tcagatgctc
    52501 agccatctcg cagtgcagcg ttgctccccg ttaactcgcg taaaggcgca gataaaattt
    52561 cagcgagtga acggaaaacc cactttggcg aaatatattg cgggctggat ccactgcaag
    52621 cgacttatga gagtgaccgc tgtgtttatt gtgccgaaaa agctaactgc aactggcatt
    52681 gcccgctgca taacgctatt ccggattaca tccgtctggt acaggaagga aagattattg
    52741 aagcggcaga actttgccac cagaccagtt ccttaccaga aatttgcggc agggtatgtc
    52801 cacaggaccg tctttgtgaa ggcgcatgta ctttgaaaga tcactcgggc gcagtctcta
    52861 tcggtaatct ggaacgctac atcaccgata ccgcgctggc gatgggctgg cgtcccgatg
    52921 tcagcaaagt tgttccccgt agcgaaaaag tggcggtgat tggcgctggg ccagcagggt
    52981 tagggtgtgc tgatattctg gcgcgcgcgg gagttcaggt tgatgtcttt gatcgccatc
    53041 cagaaattgg cggtatgctg acttttggca ttcctccttt caaactcgat aaaacggtat
    53101 taagccagcg gcgagagata ttcaccgcaa tgggaatcga ctttcatctt aactgtgaaa
    53161 ttggccgcga tattaccttt agcgatttaa cttctgaata tgatgcagtt ttcatcggcg
    53221 tggggactta cgggatgatg cgagcagatc tgccgcatga agatgcgccc ggtgtcattc
    53281 aggctctacc gttcctgact gcccataccc gccagctcat gggattgccg gagtctgaag
    53341 agtatccgct gacggacgtg gaaggtaagc gagtcgtggt attgggcggt ggcgatacga
    53401 caatggattg tttgcggact tccatccgcc tcaatgccgc cagcgtgacc tgcgcgtatc
    53461 gtcgtgatga agtcagtatg ccgggctcgc gcaaagaggt ggttaatgcg cgcgaggaag
    53521 gtgtcgagtt tcagttcaat gttcaaccgc aatatatcgc ttgtgacgaa gatgggcgtt
    53581 taactgcggt gggcctgatt cgtaccgcca tgggtgagcc ggggccggat ggtcgccgtc
    53641 gtcctcgtcc ggtagcgggt tcagagtttg aattgcccgc cgatgttctg attatggcct
    53701 ttggtttcca ggcgcatgcc atgccgtggt tgcagggcag cggaattaaa ctcgataaat
    53761 ggggcctgat tcaaaccggt gacgtcgggt atttacctac ccagacgcat ctgaaaaaag
    53821 tctttgctgg tggtgatgca gttcatggcg cggatctggt tgtcactgca atggccgcag
    53881 gaaggcaggc ggcgcgcgat atgttaactc tgtttgatac gaaggcatcg tgatgaaatc
    53941 gttaattatc gttaatccgg ctgactgtat tggctgccgt acttgtgaag tagcctgtgt
    54001 ggtcgctcac ccttcagaac aggagttgaa tgccgatgtc tttttacccc ggctgaaggt
    54061 acagcgactg gacagcatca gtgcgccggt gatgtgccat cagtgtgaaa acgccccttg
    54121 tgttggcgct tgccccgtgg gggcgctgac gatgggcgag caggtggtgc agaccaattc
    54181 tgcccgttgt attggctgtc agagttgcgt cagtgcgtgc ccgtttggaa tgatcaccat
    54241 tcagtcgttg ccgggggata cccgacaaca aattgtgaaa tgcgatctct gtgaacagcg
    54301 ggaagagggg ccagcctgtg ttgaatcttg cccgacacag gcgctgcagt tgcttactga
    54361 aagagaactc aggcgagtcc gccagcagcg tattgttgcc agcggtgaga atcctctgtg
    54421 aaagaaatct ccgcgctgaa aggcgcggag aatctattag attgcccaac cacccgcgta
    54481 aaatgcgacc agtgcggcag taatgatcac cgtaccaata ttcaactttt gccattcgcg
    54541 agcaaagaca cggcctacga ccagtgtcac aaagcccagc ataataccgg taacgatatt
    54601 acaagtcaga acgatgaaca cggcacacac caggccagcc attgcgtcaa taaaatcgtt
    54661 gaaatccagc ttcgagacgt tacttaacat cagcaaaccc acgtacatca gagcgggtgc
    54721 agtggcgtaa ccagggatca aaaatgacag cggtgataag aacagaatca gcaggaataa
    54781 cgcccccact acggttgcgg tcaaccctgt tttacccccg gcggcggttc ctgccgctga
    54841 ttcgatataa accgctgcgg gcgctgcgcc caccagaccg gagaatattg aacttactga
    54901 gtcactggtc agggctttgc cgccattgat gatctggttg tctttatcca acaaattcgc
    54961 ctgaccggcg acggcacgga tggtgccagt ggcgtcgaac actgcggtca tcaccaatgc
    55021 cagtacactc ggaagtacgg ttggctggag tgcgcccata atatcgaggc tgaaaatcag
    55081 agacttacca tcttcgccag tcagacttgg catcgccacc agaccgtggt atttcaccgc
    55141 tgggtcaaag attaagccga taatcgaaat tgcgataatc accaacaaga tcccgccggg
    55201 tacgcggcac ttctccagac cgaagatgac cgccagcccc agcaagctca tcatcaccgg
    55261 gaaggaggta aacgcaccga gcgccactgg caagccttca atcgggtttt tgataaccat
    55321 acccacaccg ttagcagcaa tcagcagcag aaacaggccg ataccgatac ctgtaccgtg
    55381 agcgataccc atcggtaaat tacgtaagat ccaggtacgc acaccggtta cggaaatagc
    55441 ggtgaagatg acgcccatca gaaagaccgc gcccagtgcg acaggaacgc taatttgttg
    55501 cccgagtacc agactgaatg cggtaaatgc cgtcaaggaa atcgcacaac caatcgccat
    55561 tggcaaattg gcccacaacc ccatcagcaa cgagccgaag cccgcgacca gacaggtggc
    55621 aacaaacaca gctgcgggag gaaaacctgc tttgcccagc attcccggaa cgacgataac
    55681 ggaataaacc atggccagaa aggtcgttaa gccagccagt acttcctgac gaacggtact
    55741 gccacgagcg gtaattttaa aataattatc cagcgcgccc tgtggctttg gtgcgtccgg
    55801 tgtttgtagg atgtctccag acataatgat gtcctcacga ggatgttaaa gttttattat
    55861 tagttgcgtt cgtacaccag acgaccatca acgtaggtgc ggtagatcga acggtcatcg
    55921 cccaacgtca tcatcacgaa caatttgtcg actaaagaaa cagagttgtc atagcgcagc
    55981 tgctgtagcg gagtggcggt gggttccatc accacgaaat cagcctcttt gccaggtaaa
    56041 aagttgccaa tcaaatcgtc aaggcccaga gatttcgctc cgccgagcgt ggccaggtaa
    56101 aacgcttcat atgccgagag gcgatagcct tgtaattgca atactttgta ggcttcgttc
    56161 agcgtttgca gcatgttgaa agtggttccg gcaccgatat ccgttcccat gcccacttta
    56221 actttcttct gccatgcttt tttcaagttg aataagccgc tgccgaggta aaggttggag
    56281 gtcggacaga aagcaatgct ggatttggtt tcgctgagac gatcccactc tttttcttcg
    56341 agatggacgc agtgagcaaa gacacagttt ttaccggtca ggccgtactg atggtaaaca
    56401 tccagataac catcatggtc aggataaagc gatttcaccc aggcaatttc atctttgttt
    56461 tcacagagat gggtatgtac ccacgtatcc ggatattctt ctttcaggcg ttgcgccatc
    56521 gccatctgtt caggagatga ggtcggggcg aagcgtggcg taatcgcata tagcagacga
    56581 ccatttttgt gccagcgttc aatcagttct ttgctttggt gatagctgct ttcggcagtg
    56641 tcgagcagat aatccggtgc gttgcggtcc atcatcacct taccggcaat catacgcata
    56701 ttgatatgac tggcggcttc aaacagcgca tcaacagatt gcggatgaac agtgccaaac
    56761 accagcgccg tggtggttcc gttacgtaaa agctgcttga tgaagaatgc cgacatttcg
    56821 cgggcgtact ctaaatcctc ataacgacgt tcagtaggga aggtgtgttt attcaaccac
    56881 tccagcaatt gttcaccata ggcccccacc atttcacttt gcggataatg gatatgtgta
    56941 tcgacaaagc ccggtactat cagtttgccg cgatagtcgc gcacgcgaat agtgtcagga
    57001 atttgatgct ttccgtcttc ccattcgcca aaccattcca cttttccctg tttaatgagt
    57061 aataaaccat cctcaataaa ccgcagcgca gaggcaatct cttccgggtt atcgaccgta
    57121 cgggtgacat caataaaact gcctcgtacc gctttcaacg tgtgttctcc tgacatcatt
    57181 gactccttta ctgttaatcc atctcttccg ctgaggtaat accaggcaga acgttttcct
    57241 gtcggtagcc accagggagg ataatattta ataaaatcgc agttaacccg ccagcacaaa
    57301 tagggttttc aactagtaca taaatagagg ctggcaatat tttaaaaatt tcgggatcgt
    57361 aggagacgcc aagtcctaaa cctaaagaag tggcgacaat aagtgtttct cggcgcttta
    57421 aaccgttggt gatgattatg cgaatcccag cgatggcaat catggaaaac atcaacgtca
    57481 ttgcgcctcc cagaactgcc gagggaatgg tcgtgaagaa gccgccaatc atcggaaaca
    57541 aaccgaggat aaccagcatt accgcgatgg ttcgcccgac ataacgtgaa gcgacgccag
    57601 tcatctgaat aaccccatta ttttgcgcaa acgtggttaa gggtaatgac ccgacagcgg
    57661 aggcgataac agaaaccaga ccatctgcca gcacgccgcc tttcagtcgg gactgatact
    57721 cttccccctg aatggggcgg cgggaaacca ttgccgtggc ggtgatatcg ccgactgctt
    57781 ccagcacgct aagcagataa atcgtgccaa ccaccaggaa ctgatggaag ctaaaactaa
    57841 agccgtattt gaacggatgc gggatggtga ttaacggcaa attgcgcata ctgctgaaat
    57901 ccaccatgcc caggcataac gatgcgatat agccgacaca tagcccaatg gcgatccctc
    57961 ccatgcgtag caacggactg cgacagcagt taaagccgat caccacaatt aaaaccaata
    58021 aaccaacgcc gagatgttcg taattgccga acgtaccgct gcttttggct gcaaatcctc
    58081 caccaaaatc gataatgccg actttaatca ggcttaagcc gatcatcagt accacaatac
    58141 cgctgacggt aggcgtaata acccgacgta aatagggcaa gataaatgaa gatccgacaa
    58201 ccagaaatgc gccaacgaag gagacgccga gaagcgacga catgattaac tcttcgtgaa
    58261 aaccgtcgct tttcatgctg ctgcccagcg caatcatgac cgtaacaaat gaaaaattga
    58321 ctgactgaat tgagagtagg ccagaaccga cgatgccgta gcggtttact tgtaaccagg
    58381 taccaatacc agaggcgatc atcgccatag aaacaagata ggcggttgtt tcagcggaaa
    58441 gctgtaaggc cgcacccacg attaacgcgg gggttaccat cggaacgaaa attgccaaca
    58501 gatgggtaat ggcaccaacg agagcctgat gaaagggagg gcgatcctcc agttcaaata
    58561 taaggtcaga acctgcatgg tttatatcag acatcccttc actcctttta aagtaaattt
    58621 gatcatctga attagtaaaa taaattccca cgaatttggg gcgcaggacg ccttgagaac
    58681 ggcttatctc attttcgcag tcactattta gtcatcgttc aaacagagaa acaggccgcc
    58741 gaagcggcca tttattactt atattttttc cagcgcggtg agtattttct ccggtgtgaa
    58801 atgccattcg cgtaaccaga tgccgcaggc atcgtgaatt gcggtagcaa tcgccggagc
    58861 tgcgccattt acaccaattt ccgagatcga tttcgccccg aacgggccga ctttatcgtc
    58921 gctcggcacc aacacagcgc ggaaatcacg cggaatgtca ccaattttcg gtgcgccgta
    58981 actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtta taaatgatct cttcgctcat
    59041 actgtggccg atagcacgca gggtggcacc gtagatttgt cccaacgcta actctggatt
    59101 gaccggtgta ccgcagtcca gcaaggcgta gaatttatct aggcggattt cacccgtacg
    59161 cgtgttgacg gcaacttcag cgaagtttgc gccatacggg aaagcgaaat caggcgtgat
    59221 ataactgcca gttcccacca gtgaaccaaa gccggtgccg gtttcgcctt tatgggcaat
    59281 atccccgaaa ctaacttcgc ctttcttgcc gcgcacgacg cccggcgttg ctagttgaac
    59341 atctgccact ggctcaccca acatttgcgc gccgtggaac agaattttct cccgcagatt
    59401 ttccgctgcc aaacgcgccg cgttacccga gaagcaagta ccggacgagg catatgcgcc
    59461 tttatcaaac aacgcatgat cggtatcacc ggagataaca tgcacgtcct gcggtgggca
    59521 gtgcagcact tctgctgcca gtttcgtcac tacagtatcc agaccagtac caatatccgc
    59581 accgccagaa tgaacgataa aggtgccgtc cgattccagt ttgatcatgc agttagcctg
    59641 atcgatatcc gggatacccg atttctgcat gataatggcg acgccgcgac cgatgtgcca
    59701 gtcactattt tgcggtttcg gtgaagacca ttggatcatc tcgcgaccct gacgcaggat
    59761 ctcttccagt gcgcagctgg cggcggaagg aacggaggtc ggcgctttac cttcaccgat
    59821 tgcaccgaga attttcagct cttgcccttc gtgtacccgg ttacgttcga taatttccag
    59881 ttggtcgatc tgtaactgtt cagccagttc cgctaatgcc atggtgatag cgaagttacc
    59941 tttcggtgcg ccataaccct gataagcacc atttgggcaa atgttgctgt agtaggtggt
    60001 gacctggaaa tcgacgttat cgcacggata taacggcagc gacagcgccg gtccgttaca
    60061 cggtacggtg agtgagtggt tgccgtaagg gccagtgttg gcgcggaaat ccatcttcac
    60121 tgccgtcagg cgaccatctt ttttcgctcc cagtttgacg gtgactttcg cgacgtgacg
    60181 agaggtgtta gcaataaact cttcttcacg ggtgtagcgg aacagtaccg gacgcccggt
    60241 cacgcaggtt gcccatgcgc acacttcttc cagcaggatg tcctgtttgg agccaaaacc
    60301 gccgccaact cgctctttaa tgacatgaac tttatgctgt ttcatgccta cgaggcgcgc
    60361 gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg tggataacca gacgatcgcc
    60421 gtccatccgg gtaaagcaga tatgtgtttc agtcgggcac tgctgtgcct gcgttgagtt
    60481 ataggttcgc tcaatgatca catcggcatc ggcaaagcct ttgtccatat cgccaatatg
    60541 accatgaata ctggcggcga tatttttgcg agggcgagaa ccgatcggga agttgatgat
    60601 catatgctcg ccgcgctggg ctgcatggct gttatcgtct tccagagtat ctggcgcacc
    60661 agcaacatac accaccggtt catcgtgcac gacaggcgca tcttccgcca ttgcttcgtc
    60721 gatcgacatt accggcttaa gcacttcata ttcaacgtcg atgagcttca atgcttcgag
    60781 cgcaatttct tcactttctg cgacgaccgc agcaacacga tcgccgacgt gacgcatttt
    60841 cttgccgaac atacggcggt caagcggtga cggttccggt gcgctctgac cacccggggt
    60901 atagtagata tccgggcaat tcaggtgagt aataacgtga acgacgcccg gtaaggcttc
    60961 agctttgctg acatccagat gagtaatcag tgcgtgagcg tgtgggctac gtaacatttt
    61021 aatgacgcag gcgtcagccg ttacgcggtc ttcaacatag cagggtttcg cctgcaccat
    61081 tttcgcggca tcagttttag gataatgctt gccaatgact tcaaggtcgt cacggaaagt
    61141 cggagcgata tcaatggtgg cctgcggatt atttttacgt gcaaccgcca gttcaatgac
    61201 ctgatagtat tgttgccagc ctgcatcgcg gctgaacaaa ccagaaagcg cgtcgtcgat
    61261 ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcg gtgataatca gagctgcagc
    61321 tggatcgtta taaccagact gcaccacgcc aacatcaacc attgcctgtt gaaccagact
    61381 taactcgttc catttgccca aagattctgc ggtacgaata tctgccttct ctaactgtgc
    61441 ggcaataagc aaggacgcgt taacgatatt accgttaaag attattgcgt cagaaccggc
    61501 aaacccgaag ccatcatcac tgttgcgtac agagtgcatt cccatgttaa acaacagctt
    61561 ttgcacgttt tcgcctggat taacggttag ctcctgaggc gcgccattta aagtaaagtg
    61621 gatgatcata cggcttcctc cccagcctgt tggcagtcgg catacaggtc ggctaccaga
    61681 actcccgtga tatagcgttt ataggccacg ctgccccgca aatcttcctg cgggaaaatg
    61741 gcgttggcga cagcctgttc aagtgcattg ccttccagat tttgtttttc gacatcatgc
    61801 agacgcagtg ctttactggc gacgccatcc agcgcaattc gcataccgtc gtggtctgtc
    61861 attgcaacgg ctgccgtcac gacggttaaa cctgcctgag aacggctaat tttgcgggtc
    61921 gcacaggtgc gatacggatc tttaatgata atttcggtta gcaggcgatc gcatgggcag
    61981 gccaggtagt cctcgattga cagcgtttcg ccgttgccaa aaaccagttc agcatccagt
    62041 gccaacagga cgggaagcag caccgactct tcctggcggg cggcgatttc accaccaatg
    62101 gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga gggcttcacg cagcgctgca
    62161 ggaataaatc gcgcatcacg cagtggctgc aagcgagaca ttgcgccaat ccgcagtgca
    62221 ccgttatccc agtcaaccca gtccagttcc agatcctgta aggaaatggc aatcttttta
    62281 tcggtacggg ttggtgtagc attgagtttg ctgcccccgg cgaaccagac ggcttcatcc
    62341 tggtagcggc gcttcagttc cagcgcctgt tcgacagagt cgggcctgaa aaattgttca
    62401 atcatcttct ttccttattt aaccctcttc cctgcgggaa gagggcatct gtcatttatg
    62461 ccagcgcatc catccgacgc cacatactgg cagcggcttt tctggcttgt gcataaatgg
    62521 aatcgcaatc gaagttaaac tgacggtctt catagaccat cacaccattg accatcacgc
    62581 tgtgaacgct gcctgagccc ataccgaaag cgatatgccc ggcgatattg tctgccagca
    62641 gcggcgtcgg tgagttgtaa tcgcaaatgg ttaaatcagc tttgtaaccg gcttccagaa
    62701 gtccaaattt cgcgccaaag ttgcggctca tcagttcgtt accgttagtc agggctttgg
    62761 caaaactgtc aggccacagc ggaccacccg catcgcgatg tttaaagaag gcaaatttca
    62821 tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa cgccagattg cggatgtcgc
    62881 taaggtgatg gttgtagccg acatggttgt tcatgtttga acgggcgtta tgcaccagga
    62941 acgcatcgcg ctgattgagt agggcgatgt catctttcga caagtacagc ccatgagcga
    63001 ccagcgtttt gctatcgatg agatcgaatt gcgctagtcg tgccagcagg tctttgccgt
    63061 accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc agcgtgaata tgcaaaccac
    63121 ggcctgtggc tttcacggct tcacgtagca tctccagacc ggcatctggc acggtaaacg
    63181 gcgcgtgagc accgatatgt gcttccacca gatacggctc ggttgctgct ttcttcgcct
    63241 catcaatctg acgggcgaag cggatgtttt cttctacacc ttcctgcaac tctttgatgc
    63301 cgttgttacg gtcagtagtt tcaaaacagg tcatcgcgcg caggccaact tttaaaaatg
    63361 cgtcgcgcaa tgtggagagc gacccgccga tatacgccgg agaggcatgg tgatcgataa
    63421 ccgatgtaca tccgctctta atcgcttcca gggaacaaat cagcccgctg taatagagcg
    63481 actcttcatc aagggcgcga tcgagccgcc accagagatt tttcagcgtt gagatgaaat
    63541 ccgggcaggg ggcgatgttt gccataattc cgcgggaaag ccccgagtaa aaatggttgt
    63601 gcgagcagac aattcccggc atcacaatcc gaccatgcat ctctttgtag ctggcatcgg
    63661 ggtagcgttg cgtcagggca tcgccgatag cgacaatcac atcattttcg atagcgatat
    63721 caacgccttc ctgcactttc gccgggtgta actgcactgc ggtgacattc ttcagaatca
    63781 acatgattac acctccacgc ggcccagcag ataatgatga tgctggtgga catggctgat
    63841 gatgcggcac atatcgttca gctccggtgg tacgttgttg aactgacctt cgctgtcgat
    63901 gtttaacacc cagctttggt tattcagtcg tactcgtacc cggcaatctt ccacaaggaa
    63961 gcctgggttg ctgctgttat caaagtcttg cgccaggctg aaaacggtga ttttgtcttt
    64021 gtatggttta ccgttccacg gacagaactg agcgcagttg ccgcattcgt tacagtaagc
    64081 gtcgaggtgc agcgtctgga atctattctg gaagcctggg accgcaatgg agacgttggc
    64141 gcggttcggg cagacatcca cacacttgct gcaaacgtag ttacattcga ggcagcgagc
    64201 ggcttcctgg gcgacaaacg cgtcacgatc gtcactgttc accagagtga tagagatatc
    64261 gcctttacgt tgatagattt ccgccggatt gacgttgttc cagtatttat cgttctggtg
    64321 ggaacggata ttttcccgac taaggatggc atcggtcgcc cgacgcgcgg ttccgacagc
    64381 agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc ataaagacgt cagtcagacg
    64441 agtttcgcca ttgtggtcga cgtctggcca gccgtttttg tccagcggca cgcccatcgc
    64501 attcagggct tcagtatcct gctgttcacc aatggcggtg atcaggctgt ctacatgcag
    64561 tgttactgtt tcgttggttt caaccggacg gcgacggcct ttctcatcgg gttcgccaag
    64621 cgacataacg cgcaaggtta aggtgccatc agcatcgaaa cgttccggat tattcaggaa
    64681 acggaactcc acgccgtcgt gcaacgcttc ttcatactct tcgcgccatg cgggcatctc
    64741 ttgcagtgaa cgacggtaaa cgatcgttgc tttttctacg cctggaacgc gtaacgccgc
    64801 acgagcgcag tccattgcgg tgttacccgc cccgacaacg accacatgtt tgcccagctt
    64861 gagcgctgta cccttgttgt attcacgcag gaaggggagt gatttccaaa cattttggtt
    64921 gtcacccgcc agtttcacac cgctattttt atcagtgccg gtggcaatca gaacatagtg
    64981 gaagccctga ttttttaact gttcaacggt caaatcgggt gagcagccat actcaaattt
    65041 cacgccgtga tcggcaacaa aatcgatatc gtgctgaatt aactcagccg gaattcggaa
    65101 ctgaggaatg atatttttca ccacgccgcc cgcattggct tcgcgttcaa acagcgtaac
    65161 cggatggccc gctctggcaa ggaagtaacc tgctgccaga cccgccggac ctgcaccaat
    65221 cacggcaacc ggatggcgtg aaccagaacc ggctggtttg tgccagcgtt gcttatattc
    65281 atcccaacct ttttccagcg cgactttttt cagttcgcgg atattcagcg cactgtcgta
    65341 atccaggcgg gtacagttgt attggcactg gtgatcgcaa atatgaccgg taatggcggg
    65401 cagagcgttg cgttggtaga tgagttccag cgcgtcggca tagcggtgtt cgccaagcag
    65461 acggatgtat tccggaatat cttgcttgat agcgcaggca gtaacacagg gggcaacgta
    65521 gcaatcggtc agcggcaggt cttctgccac ttcaatacgc tcttctggct tccagtgttt
    65581 ctgggtgtat tccatggtta acgcatctgc tgccagtctg ttcagtcgtt cgacgtcaac
    65641 atggtcaagt ccccaggcgt cggagccttc cagctcgcgc atgcaggcac ttaagcgcaa
    65701 atagccgcca ggtttcagca ggtcggttgc catagtaata gggcgaatac ctgtatcaaa
    65761 aatatcgcgg atagtcagct gactggcacc accggaataa gaaatgggca gtttgccgtc
    65821 aaaggcgcga gagagaactg ctgcaacatt gatggagagc gggaacagcg cacggcctga
    65881 catatacatc tcttcaccag gcagtgcgcc tttattgttg atggtgccga gagtgttagt
    65941 cagttttacg ccaaagccga gtgatttttc ttttgccagt gccatcaggc gttccagcat
    66001 ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac tcttctttta agcctatgta
    66061 accgaaaccg cagacatcga ggatctcacg aacacgcgcg tatcccagta aggtcgggtt
    66121 aagtttcaca aaggtgttga gccctttttc ttccagcatg tagcggcaaa tggcttcgat
    66181 ttcatgcgga ggacagccgt gcatggtgga gagggtgacg ccctgcacca tactggtggg
    66241 gatgcgagcg ggtaaggctt gcaagctttc gcgtttttcc tgcaatccgt ggcgggctaa
    66301 aaatgcgtca tcctggagta atttattcag cgtatcgcga tattgcgcga atttcggatg
    66361 gtcagatgcg tccatcatat tgtcgatgaa ctgttgcatt ggcggttgct taataccttc
    66421 gaggttgtaa ccgacgctca tattaaagat gaacgattta ccggaatcag aaggctggaa
    66481 catcgcttcg agaaggtgca gggcaaacca ggctttgagg tattcatccc aggctttaag
    66541 cagggtaaat tcggtagacc attcggtgtt aaagcactcg tcttcggcat cgatacaggg
    66601 cttttccagc tccaggcggt caagaatttg gacggttttt agttcgatga agcgtccgcc
    66661 agtcagccag gaagtgacaa tattttgcgc gagctgcgtg tgcgggccag cggcagggcc
    66721 gacgggagtg gcacaggttt caccgaagac gctaacagtt ttacctttta caggtgagta
    66781 aaactgttgc tcgggaatac caaagattga gcgttgttgt tggtattcat caaatatgcg
    66841 cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc cccataaccc tctccttagc
    66901 ttatacagat atcattgagt gaccggtgtt gagaaatccg gtggtttagg gggatagagc
    66961 aacaatcgca ccactcttgc caggtttggc gggaaatata aaaaaatgtg aggcggctcg
    67021 aaacttaaag gctttttgtg aatgaactca caggtgccaa atgagaatta aaatcactcc
    67081 tgaaaattat tattgtcagc ccatttatca ctttttctct tttctcaaaa tcagtgattc
    67141 cttatcatta tgataaagag tgctggagag attaaatatt tgaacgcaat cgtttaacta
    67201 cattttctaa attttagtat accttttttg cgctgtaata aagtcgtccg gagtatcaat
    67261 atctaaaatt atttctgcat tttctatctc gacggaataa tgagctccca taaggagtgc
    67321 ctgacgcata ttggtgacat tgggtcgttg gattgcctgc atcaagcatg gttttgacac
    67381 taaaatcgga tggccgggga tgccattatg gagtggcagt attgcgccat catttcgtaa
    67441 cgaccagatt tttctaaaaa tatcgatggt gagggttggc atgtcaccgt gggtgagaaa
    67501 acaatgttct gtttgcactg cgggtactgc ggcctttact gatgttagta aaccctgcgc
    67561 ataatctggg ttgtggataa tagtgatatt gctctggttc gcataacgtt cgtgcagttc
    67621 attaccacga tagccggtga ctaaaataat tcggctacaa aactgcaacg catttttgat
    67681 acttgtatca agaattgtcc cctgttgcca gggtaacatc attttccatt gccccattct
    67741 tgatgataat ccggcagcgg taattataca gtcgatggct gacatagtgg ttccattatt
    67801 tataggggct aataacgcct gataattgcg ttacttcctg atggattaag ttggggataa
    67861 tttatcccag agaggtcata aagactcgtg aaaagtatag ttgacccatc ggcattagtc
    67921 attgatttag gtgcgcagaa acgtcccact gtgatttctg ttgttggtgc tggggggaaa
    67981 accagcttgc ttttttggct ggcagaactg ctccaggcta gcggcaggcg tgtgttaatc
    68041 acgacgacga cacatatgtt tatgccaacg tctcactggc ccgtggtttt ctgtcgtgat
    68101 cccgccatgc ttcctcatgc gtctcttaca tcccccattt cattttgttt tcacagctgg
    68161 aaagcgaacc agggaaaagt gcagggattt acgccagaag cgattgatgc actggtgcaa
    68221 cgaccagagt gtgacgtaat tctcattgaa gcagatggct cgcgtggaat gccgttaaaa
    68281 gcgcctgatg agcacgaacc ttgcataccc aaaagcagtt gctgcgtgat tgctgtgatg
    68341 ggaggacata ctttgggcgc gaaagtgagc acagaaaatg tccatcgctg gtcgcagttt
    68401 gctgatatta ccgggttaac acctgatgca accttgcaac tgagcgatct cgttgcgctg
    68461 gttcgccacc ctcagggggc gtttaaaaac gtaccgcaag gttgtcggcg agtctggttc
    68521 attaaccgtt tttctcaatg tgagaatgcg attgcacaaa gcgagctcct tcaaccgctg
    68581 caacaacacg acgtagaggc aatctggctg ggcgatatac aagaacatcc tgcaatcgcg
    68641 cgcagatttg tgaattagcg gcccgggtat tccgggttac tgaaaggata tccattgagg
    68701 ttggtatgaa tattttcaca gaggctgcaa aactcgaaga gcaaaattgt ccgtttgcga
    68761 tggcgcaaat tgttgatagc cgaggctcga ctccccgcca ttctgcacaa atgttagtgc
    68821 gcgccgatgg ttctatcgtc ggtacaattg gtggcggaat ggttgagcgg aaggtgattg
    68881 aagagtcact tcaggcattg caggaacgta agccgcgatt attccatgga cgtatggctc
    68941 gtaacggtgc ggatgctgtc gggtcagatt gtggaggtgc aatgtcagta tttatcagcg
    69001 tccatggtat gcgtccacgt ctggtgttga ttggcgcggg gcatgtcaac cgggcgatag
    69061 cccagagtgc ggcgctatta ggatttgata ttgccgttgc cgatatttat cgcgaaagcc
    69121 tcaatcctga actattccct ccatcaacca cgcttctcca tgctgagtcg tttggtgcag
    69181 cagtggaagc actggatatt cgccctgata attttgtcct gattgccacg aataatcagg
    69241 atcgtgaagc cctcgacaaa ctcattgaac agcccattgc atggttgggg ttgctggcaa
    69301 gtcgccgcaa ggttcagctt ttcctgcgtc aattgcgtga gaaaggcgtg gctgaagaac
    69361 atattgcccg tttacatgcg cccgttggtt acaacatagg tgcggaaacg ccgcaggaga
    69421 tcgccatcag cgtgctggca gaaatattac aggtgaaaaa taacgcgccg ggtgggctga
    69481 tgatgaaacc ttctcatcct tccggacacc agctggtggt gattcgcggt gcgggagata
    69541 tcgccagtgg tgtggcgcta cgtctgtatc atgcgggttt taaagtgatc atgctggaag
    69601 tggaaaaacc gacagtgatt cgttgtaccg tggcgtttgc ccaggccgtg ttcgatggcg
    69661 aaatgacggt cgaaggcgtt actgctcgcc tggcaaccag tactgcggaa gcgatgaaac
    69721 ttaccgaacg cggattcatc cctgtgatgg cagatcccgc ctgttcattg cttgatgaac
    69781 tgaaaccgct ttgcgtggtg gacgctattc tggcgaaaca gaatttggga acgcgggcag
    69841 atatggcacc agtaacaatc gcgcttgggc cgggctttac tgcagggaag gattgtcatg
    69901 cggtaattga aacaaatcgc gggcactggc tcggtcaggt gatttactct ggttgtgcgc
    69961 aggagaatac cggtgttcct ggcaatatta tggggcatac cacccgacgg gtgatccgtg
    70021 ctcctgctgc aggcattatg cgatccaacg tgaaattagg cgatctggtg aaagagggcg
    70081 atgtgattgc ctggattggt gagcatgaaa ttaaagcacc gttgacgggg atggtgcgtg
    70141 gcttgttgaa cgacggcctg gcagtggtcg gtggttttaa aattggtgat atcgatcctc
    70201 gtggtgaaac ggctgatttc accagcgttt ctgataaagc ccgggcgatt ggcggcggcg
    70261 tacttgaggc gttaatgatg ttgatgcatc agggcgtgaa agcgacaaaa gaagtgctgg
    70321 aagtggctta aatgaaaatg gccggatggt attacccacc cggccttaaa attactgaca
    70381 aatcaccgtt ccggttttgc cttcaatccc ctctttcgct ttgctcaata cggtaatcag
    70441 tgcttcacga cctgcgcgag agcgagcaaa cgaggcggca gcttccactt taggcagcat
    70501 agaacctttc gcaaaatgac cttcttcgat aaagcgttca gcatcactta atgacaggcg
    70561 atcgagccac tgttcattct ctttaccgaa gttaatggcc actttttcta ccgcagtcag
    70621 aatgatcagc atatcggcat caatcatttc tgctaaacgg gcgctggccc agtctttatc
    70681 gataaccgcg ctggcaccac gcagatggtt accttcacga ataacaggaa taccaccacc
    70741 gccaacggtg atcaccacct ggcctgcatc taccagagct ttaaccgttt ctttttcaat
    70801 aatatcaacc ggttttggcg aggcaacaac acggcgatag ccgcgaccag catcttcttt
    70861 cagagtgtaa ccctgttttg tcagttgctc tgcttcttgc tcggtaaaga acgaaccgat
    70921 cggcttggtc gggttgagga atgccggatc gttagcgtcg acttcgacct gagtaaccag
    70981 cgttgctacc ggtttattaa tgccccgaga aagcagttct tctcttaagg cgttttgtag
    71041 atcgtaacca atataaccct ggcttaaggc aacgcagaca gacatcggca gcattggcga
    71101 gtgcgcttca gtttttgcag cagcttcaaa ggcctggtta atcatgccca cctgcggccc
    71161 gttaccatga gtgacgataa cttcatgtcc ttgggcaatt aaatcaacaa ttgcctgaga
    71221 agtaattttt accgctttca tttgtccggc caggtcatcg cccagcgcat tcccgcccag
    71281 ggcgagaaca attttcttac tcatttttcc tcactcatca ttcttgattg tgtatagatc
    71341 cctgacagtg gttagagcac gggagggaca aacggtttgc gtcgcaggaa acgacctcgc
    71401 ccggctttgc ctgtaaaggt gccgtcacag aaaatcgttt caccacggga taaggttctg
    71461 acaatcgcgc cctgacaggt aaaaccctcc catggcgagt agtcggcgtt gtcgtggaga
    71521 tggcgatgct gaatttgttg gctctgacgt gggtcgataa tcaccacgtc gccatcggaa
    71581 ccgggcgcta ataatccttt ttgcggccac aggccaaaca gcctggcggg cattgcgctg
    71641 gttaattcaa caaagcgttc cggtgttata cgtcccgtca tcacgccact ggaaaacagt
    71701 aactgcatgc ggttttccac accgggtaag ccatttgggc agcgactgaa atcgccttta
    71761 gaaatttgca ggcgttgagc catcgagaag gtgcagtgat cggtcgccac cacgtcaatc
    71821 gcaccatcgc tgatgccaca ccacagtttg tcctgctcgc gtacgttacg cagcggtggg
    71881 ctaagaatga acttcatgcc gtcttctgta tcgtaactgc gttcgtccaa caggagatat
    71941 tgtgggcagg tttcaaccca gactggctgg tgattcgcac gggcaagacg cagataatcc
    72001 agacctaagc cgttagacag gtgcacgata tagagcgggg cgttaccggc aatttgtgcc
    72061 aggttaatca tgcgggcgat ggcttccgct tcgcattcca gagggcgact caaggcatga
    72121 tagcgcggcg cggttaaccc tgcggcgata aattccgccc gcttgctggc gatagccgca
    72181 tcattttccg ggtgcacggt ggtcagcgcg ccggattcat gcagacggcg taatgcctgc
    72241 aaaacctcgt catcgttgag tttgtattga taggttaaat agagtttaaa actgctcagt
    72301 ccttcctcga ccatcatcgg gatttcgtcg aggattgcgt gattaatgtg ctggatcaca
    72361 ccgtgaaagc tgtaatcgat gaccgcttta tgggcggcat aaccacgata aacctccagt
    72421 tgatggcgta accgacagcc gtttgggcca aatcccatat ggtcaataat ggttgttgta
    72481 ccgccacacg cagctgcgcg ggtaccggta aaaaaatcat cacaactgcg cgcgatgccg
    72541 acatcaatat tgaaatgcgt atggacatcc acgccgccag ggaaaacgta acagccagtg
    72601 gcatcaattt cttcatacgg gagctgcggc gaaatattgt tgcccaactg gcgaacaatc
    72661 ccgctttcaa tcagcaaatc ctgtttggct tgtccatctg cgttaacgac agtgccgttt
    72721 ttgatcaata cgcgcatagc aaactccaaa aaccctccgg cgaaccggag gggaataagt
    72781 gaagagtaat tactctgttg ccagccagct taacgggatt gcagcgtaca tcgcggcaca
    72841 ggttaccagg tgagatttcc aggttttttc gttaggtgca tgcgcttcag gttctttacc
    72901 cgggccaaag ccgatcaccg gaatgccgtg acggcccatg atagatacgc cgttagttga
    72961 gaaggtccac ttatcaacaa ccggcgcttt gccaaacagc ccttcgtagg cattcaccag
    73021 tgctttaacg gtgaagtgat cttcttccac tttccaggtc gggaagtagc attcggttgg
    73081 gtaaaccagg ccagtccagg acggacggtc gtagttgtac atagaaacaa ccgcgttagc
    73141 tttctgtact gcaggcaggg cgcggatttc gtccagcgcg ccttcccagg tttcgcccca
    73201 ggtcagacgg cggtcaatag agacggcgca gctatctgct acagcgcaac ggcttgggga
    73261 tgtgaagaag atttcagaaa cggtgagggt gcctttgccg aggaattcgt cataacccag
    73321 acgttgggag agttcttgta attcgccaag aatcggaccc attttgaaaa tggcgttgtc
    73381 accgcgttct ggcgcagaac cgtggcagct aacaccctga acatcaatac gaatttccat
    73441 acgaccgcgc tgaccacggt atatctggca gtcggttggt tcggtactga ccacaaattc
    73501 cgggcgaatg ccggattgtt caataatgta ctgccagcac agaccgtcgc agtcttcttc
    73561 ctgcacagta ccggtaacca gcagggtata ttcatcttcc agaccgaggt ctttaatgat
    73621 tttaccggca taaaccatag atgccatgcc gccttcctgg tcggaagtac cgcgaccacc
    73681 gattagctca tcagtttcca tgccttcgta cggatcgaag tcccagtttt tgatgttgcc
    73741 aatgccgacg gtatcgatat gagcgtccat tgccaccaga cgcgggccgt ggccgatata
    73801 accgagaacg ttgcccatcg ggtcgatttc aactttatcg aagccgactt tttccatctc
    73861 ttctttaata cgatgtacta cgcgtttttc gtcgcagctt tcactgggaa tagcaaccat
    73921 gtcgcgcagg aagcgagtca tgtccgcctg gtaatctttt gctttttcaa gaatcagttt
    73981 gaatggaata ttcttagcca ttatttttct ccagattttt tactccccgt tttgcaggga
    74041 gtagttccaa tagagtgatt aaggtgctac agcgtgtttg ccttcccaga caacttcgcg
    74101 gtagtgcttc acgtcggtgt cgccttcagt gctgataacc agcaccacgg catctttgtt
    74161 cagcgccagt ttttccatca ggctttgacg ttgcgggtga taatgaaccg ctgcgagaac
    74221 gcccaaaccg acagcgccgg attcaccgga gatgatgcgc gggtcgttgc cgtacggatt
    74281 acccagcacg cgcataccta atgcggcaac gctgtcctgg caggagatga attgggtggc
    74341 gcagttacgt aggatttccc agcccagcgg gttaggttcg ccacaggcca ggcctgccat
    74401 gatggtggcc atatcaccgc caacgttgac gatgtcgcct ttgacgccgg agcgataaat
    74461 acagtcagct ttgtcaggtt caacaataat gctgtgcaga ttttgcgggc tatagacgtc
    74521 gaccagataa cccagcacac caccggccat tgctccgaca ccggcttgca gcagaacgtg
    74581 cgtcggggtt acgcccattt cacgcatttg ctcgacggct tcatctgcca gggttgcgta
    74641 gccttgcatg atccaggttg ggattttggt gtaaccttcc catgccgtgt cctgtaccac
    74701 ttcccagccg tgctgctgcg cgtgttgcat ggtcaggcga acggtatcgt catagttcat
    74761 atctgtgacg atgcactcgg caccgaggtt cagaatggcg tcaacgcgtt cctgagcaga
    74821 acctttcggc atgtaaatca ccgcattctg tccgagttgc tgcgctgccc acgccacacc
    74881 gcgcccgtgg ttgccgtcgg tggtggtcgc gaaagtcatt ttttcgccga tggcattttt
    74941 caggtgctca aatgacagcg tttcgatatc aagatgatat ttttcacaca ataattgagc
    75001 gatagcgtac gcaccgccga gcattttgaa ggcgttcaga ccgaatcgtt ttgattcgtc
    75061 tttgacaaga attttcttca ccccaaaaag gtttgcgaga tcgtccagcg cacaaagcgg
    75121 tgttgggcga taaccagcta ttttctggtg gaactggcgc gccagtttgg cctggctttg
    75181 cgaaaagagc ggtgatgttt cgccgttgaa aaatttgtta tcggcgatat caatcttcaa
    75241 tgagaaaacg gacatatcct atccttttgg cctctggaaa tagagggccg accagctgat
    75301 cggcccgagg ggttatttca cgcgttcttg cgcttctttc agcagttgct cgagcagtgc
    75361 acctggtttg gcgtatttac gggacaggat catcgcggcg atgatataag gcttccagct
    75421 ggcttctttg taggtagcga tacggtattt ttcgaatacg ccttcagtca cttccccttc
    75481 tttgcaggat acgccgctga tatcagctgg caggcagtgc atgtacaggg cttcgccatc
    75541 acgggtcagt tccatcatct cttcagtaca atgccagtct ttgtgttgcg cgttctgtgc
    75601 cagacactgt ttttccagtg cttttaagcc ttcgtgatcg ttagcgcgca gcaattcggt
    75661 acgctgttcc atcactttgt aaggtgccca tgacttcgga taaacgatgt ctgcatcttt
    75721 gaacgcttct tccatgctgg tgacctgacg gaagctaccg ccagatgctt tagcattgtt
    75781 tttcgccact tcaaccacat ccgggatcag gtcgtagcct tccggatggg ccagggtgac
    75841 atccataccg aagcgagtca tcagaccgat gatgccttgt ggtacagaga gcggtttgcc
    75901 atagcttgga gagtaggccc aggtcatggc gattttttta cctttcaggt tttccagtga
    75961 accaaagtgt tcacgtaacc acgcgaggtc agccattgac tgagtcgggt ggtcaatatc
    76021 gcattgcagg ttcactaaag ccggacgctg tggcagtaca ccctgcttgt aaccgtcatc
    76081 aagtgcagcg ccaacttcac gcatataggc gttgcctgcg cccagataca tatcgtcgcg
    76141 aataccaata gcgtcggcgc agaaggagat catattggcg gtttcacgca cggtttcgcc
    76201 gtgagcgatt tgtgatttgc cttcgtcgag atcttgttgt gcaaggccga gcaggttaag
    76261 cgcggaagca taagagaagc gggtacgggt ggagttgtcg cggaatacgg aaatacctaa
    76321 tccactatta aagactttgg ttgagatgtt ttcagcacgc agtgctttta atgctgcggc
    76381 aacgtccagt acttgtttca gttcatctgg cgtctgttcc cacgttaaca aaaaatcttt
    76441 ctcgtgaagg tgagaggtca gcgaattgat atccttaatc agctcattaa cagttttcat
    76501 cataaagtcc cggtagtgga atgacaggca aagtggttta tttcactgtg acttgccaaa
    76561 gtgattggtg ttttcacagc tatacctggg actaacaata agtgtgccag aattatccac
    76621 ggggctgcaa aagactaatt tgacggataa tgtgtggggt tgatcacaaa ttgatgccag
    76681 cttccggttg tttgtaaaat acgaaaacgt ttggcttttt ttatcgcgtt atttatttgc
    76741 tgctaagtat cagaaataag ataaagggac aggctatctc tcttcagttt gataaactca
    76801 ccgccgtaat tctcatattg atatagaaat gtgatatctc ttccataatt tcattagaaa
    76861 atgataaaaa tcttatgttt ttatcaaagc caatttcgcc tgagttaata tcatcatgat
    76921 ctgtgaacca tcaacgtctt caatgatcga cttttaagta agtctggaag gtaaagctat
    76981 ggagcttgct actacgcagt cagtattgat gcaaattcaa ccgacaattc agcgttttgc
    77041 cagaatgctt gccagcgttt tgcagcttga ggttgagatc gttgatgaaa acttgtgtcg
    77101 cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc cagttgagcg gcaactcacg
    77161 cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt ctcgcttcga
    77221 tcccctttgc gaaggttgcg atagtaaaga aaattgccgt gaaaaagcat ttctgggtac
    77281 gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt ttgattgccg ttacccacga
    77341 gcaacaagag catatcagtg ataatttacg cgaattttct gattatgttc gccatatatc
    77401 caccattttt gtttcgaaac ttctggagga tcaggggcca ggagataaca tcagtaaaat
    77461 attcgcgacc atgatcgata atatggatca gggcgtatta gttgttgatg atgaaagtcg
    77521 ggttcagttt gttaatcaga ctgccttaaa aacacttggt gttgtacaaa ataatattat
    77581 tgggaaacct atccgtttca gaccattaac atttgagagt aattttactc atggacatat
    77641 gcagcatatt gtttcgtggg acgataaaag tgaattaatc attggtcaat tgcataacat
    77701 tcagggccga caattatttt taatggcatt tcaccaatcg cataccagtt tttctgtagc
    77761 aaatgcacct gatgaaccac atattgaaca attggttggc gagtgccgtg ttatgcggca
    77821 attaaaacga ctcattagcc gtattgcacc cagcccatcc agcgttatgg tggttggtga
    77881 aagcggcacg ggtaaagaag tcgtcgcccg agcaatccat aagttgagcg gaagacggaa
    77941 taaacccttt attgctatca actgtgccgc gattccggag cagcttctgg aaagcgaact
    78001 gttcggttat gttaaaggcg catttactgg cgcttctgcc aacggtaaaa cagggttgat
    78061 tcaggcggcg aatacgggca cgctgtttct cgatgaaata ggtgatatgc cattaatgtt
    78121 gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt ctaccgattg gtgccagtag
    78181 cccaatacaa gtcgacattc gcatcatttc tgcaactaat cagaatttgg cccagttcat
    78241 tgccgaaggt aaattccgcg aagatctctt ctaccgactt aatgttatcc cgataactct
    78301 gccaccgctg cgtgaacgtc aggaagatat tgaactattg gtgcattact ttttacatct
    78361 gcatacccgt cgtctgggat cggtttatcc tggcattgct cccgatgtcg tcgaaatatt
    78421 gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc aatttgatgg aatatctggt
    78481 taacgtagtt ccttcaggtg aagttatcga cagcacgcta ttgccgccaa atctgctgaa
    78541 taatggcaca acggagcaaa gtgatgtaac agaggtcagt gaggcgcacc tgtcactcga
    78601 tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg aggcgctttc
    78661 acgtcataac agcaagaagc aagttgctga tgaactgggc atcggcattg ctacgctcta
    78721 tcgcaagatt aagaaatatg agttgttaaa cacataacct cagtgaatcc ggcctgaatt
    78781 caggccggat atccttttac ttcgttttct cgcaatccag aactgtattt acaatcatct
    78841 gataccccgt gcagcgacaa agatttcccg ccagtccgcg acgaatttcc gtaatggtta
    78901 atggcttctc gcgtggtttc gccagcattg ccgtggtagc cataatcagg ccaggcgtac
    78961 aaaacccgca ctgcactgcg ccggatttcg cataagcctg ctgaacatga gaaagttttc
    79021 cgcctttcgc ttcaccttcc agcgtgcgga tctcttttcc ttcagcccag gcggcaaggt
    79081 ataagcaact gtctattgct gtgccgtcga ccaacaccgt acaggcacca cattcaccca
    79141 cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg gagtaattcc gagagcggcg
    79201 tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat ggtgcattcg atggtaattg
    79261 tttcgctgtg attcattgca attttccccc cgccgcggcg acggcttcgc taatcacttt
    79321 tttggtcatc gtctggatga gatgcagacg aaactcttta ctggcccgcc atgaagaacg
    79381 cggggcgaca tcttgcagga cagattcgct gatagcttcc agcgtttgca ggtttaatgg
    79441 cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg cggcaacacc
    79501 aaatgccagg cgtaattcgc tgaaattgcc gttatccagt cggcaatgtg cggcgcagcc
    79561 aatcgttgaa atatccattg cgtcgcgcat ggcatattta aaatgcgcgc tgcccgcgtg
    79621 ttctttcggc tgtggcggaa aatgaaaggc gacgaggatt tcgtcatgct caagagacac
    79681 tttgcccggc ccggtgtgaa agccattaat cgggacgaaa cgaacaccgc gcggggagtg
    79741 gatctccagt ttcgcgtcat aaattagcgt tggcgtggca gaatctgcgc tggtggcacc
    79801 gttgcaaata tttccaccgt aggtagcgac gttacggatc tgcggtccag caatggacgt
    79861 ggccgcagca cataacgccg ggagatgacg ttgagttata ggatcttcta ttagctgggt
    79921 aaatgtcgtt gcagagccga tacgtagcga gccatcttcc gccagcgtaa ttccccgcag
    79981 ctccgccaga ttatgaatat caacaatatg gcgataacgg tcattgtgat ggtggagctg
    80041 aatcagtacg tcagtgccac cggcgagcag tttggcctgc gggttgtcag ccagcaggtt
    80101 gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca aaatcaaaca tgatgttatc
    80161 ctcaaatcaa tcctgccaga tggaactctt catataaccg ttttggcgtc agcggcagtg
    80221 tattgattgc aacaccggta gccatcttca ccgcgttacg aatagcagcg gcaacaggaa
    80281 ttattggtgg ctcacccagt gacttatgtc cgtatgcgga ttgcggctca ttgatttcga
    80341 cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg cattttgtaa tccagcagat
    80401 tggggttacg gaccacgccg cttttagcat cgatgatcat ctcttcaaat agcgcccagc
    80461 caatgcccat tcccattccg ccgtgtacct gaccttctgc cagcagtgga ttaagaatat
    80521 gccctgaatc atgaacgttg aggatgcggt tgatggtgac tttgcacagc gcaatatcga
    80581 ccgtcagatc aacaaaggta cagccaaacg ccggtgggtt agtggtggtt ttgatggagc
    80641 tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc gccaaatctt
    80701 ttaacgacat taacggctct tccggtcgtt caaccagcac gatatggcct tttatcaggg
    80761 tcagattcat cgctgactga tgtagcatga ctgcggcgtg agcgatgatt ttctctttta
    80821 ataatagtgc cgcactgcgc agcgcaggcg cggcaacata gctctggcgt gaggcaaatg
    80881 cgccgggatc gaacggcgta acgtcggtat cttgagttga aataacgcga acgtcgctga
    80941 ccggaacccc cacggtttct gccaccattt gcgagaagac ggtgtcggca ccctgaccga
    81001 tttccgtcgc gccgctttgc acgttgatgg ttccatcctg attcatcaga aggcgcgcgc
    81061 ctgctatttc tacgccgaca ggccaggtgt tagaggtgta gctaaaacag gcgacgccaa
    81121 cgccgcggcg caaattgcct tgctggttct ggcattctgc acggcgtttt tcccattcaa
    81181 agattttccg gcctttttca agacactccg gcaaccctgc gctgtaaata cgtttgcccg
    81241 tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca acaggatcaa
    81301 tacctaacgc tgtcgcggcg tcatcaagca tagactcaac ggcaaatacg acttgtggcg
    81361 cgccataacc acgcatcgca ccagccgagg ggaggttggt atagcaggtc tttgaactgt
    81421 aagcgtaggc acaacgagga taaaggtaag cgactttatt cccccccgca gaagcgatgg
    81481 agtgcccgtg agatgcataa gcgccggtgt tagacagaac atccagacta taacctttca
    81541 atgttccgtc gcggttcacg cccatttgcc cgtcaatggt aaaagcgtgg cgggtacggg
    81601 ttgcgaggaa acactcttca cggctaaggg aaactttcac cggaatgccg ccaagcttgc
    81661 tggtcaggaa tgccgccatt ggctcttcca gtacatcctg tttattacca aaaccgccac
    81721 cgacaaacgg tttgatgact cgtacgcatg accagggaat atccagcgcc tgaccaacca
    81781 cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc gagtcatcct
    81841 ccatccacgc cagcgatgtt acgctttcca tgtgacaatg ttgaataacg ggggtctgat
    81901 agtgcccctg tacctggtag tcggcggcat cgattgtttg ttggacatta cccgtcgaca
    81961 tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg tgcagcgtct tctgccagcg
    82021 ccgcttctgg cgtggtgata acgggtaatt cttgccactc aatgctgacc aattgcgccg
    82081 ctttttctgc cgtgagttca tcgcgggcca cgacgatggc aacggcgtca ccatgatgac
    82141 gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg cttgttttcg tcaagtgtcc
    82201 aggcatgccc agcagtagca aatggaatat caggcacatc ttcccaggta aaaatcgcca
    82261 gtacgcccgg caaacttctg gcttgttcat cattaatact tacggcataa ccatgtgcga
    82321 tagggctacg tacatatttc gcataacaca tgcccgccat aacataatcg tcagtatatc
    82381 gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat gcatgattca cccgtagcgg
    82441 ttgcttcccg cgcttccata gaaatcccct cgatttgaac atttggttct tatggcaaag
    82501 atttagtcta agcaggcaag atttacgcca gaaacgacga taagcgttag gggaggtagg
    82561 aagtgtgatg acgttaaaaa aacacagata tgatacgaaa caggactctg aataggggca
    82621 gtgatacgta tcaatatgag tggcgataaa atcaatgtga taaattggga ttattacgca
    82681 tcaggatggg aaatagggta aaaagtcagg aagttcgata cctctacagc gaatattttg
    82741 ctgatgatac ttccctttgc caaatgaaat taacctcttt ctctattacc acgtttttcc
    82801 agaagcaaga gattgggtat caagaggctg gctgctatga taaggcgtct tgttttttaa
    82861 gcgaggaaag attttgagtg cgggacgcct gaataaaaaa tctctgggta tcgtgatgtt
    82921 gttatcggtt ggactgcttt tggcgggctg ttcgggtagc aaatcatccg atacaggaac
    82981 gtattccggc tccgtttaca ccgtgaaacg gggggatacg ctatatcgta tttcgcgcac
    83041 cacgggaacc agcgtaaaag aactggcgcg actgaacggc atttcccccc cttacaccat
    83101 tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt agcagtagta cacgtaaatc
    83161 aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg tcatcagcgg taccgaaatc
    83221 ttcctggccg ccagtagggc aacgttgttg gttatggcca acgacaggga aagttatcat
    83281 gccgtattcg acagcagatg gcggcaataa agggattgat atctcagctc cacggggtac
    83341 acctatttac gccgcgggtg caggaaaggt ggtgtatgtg ggcaaccagc tgcgtggcta
    83401 cggtaatctc atcatgatta aacacagtga agattacatt acggcttacg cccataatga
    83461 cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg caaaaaatcg ccactatggg
    83521 gagcacggat gcggcatctg ttcgcctgca tttccagatt cgttaccgtg caacggcaat
    83581 tgatccgcta cgttacttgc cgccgcaggg cagcaagcca aaatgctgat ggcgaattaa
    83641 tcagcagtca gcagcgcggc acttgctaag gagagcgtaa ggtttataat gccttacgca
    83701 tctcgaagcg ggcgtagttc aatggtagaa caagagcttc ccaagctcta tacgagggtt
    83761 cgattccctt cgcccgctcc atgaccattc ttaaagtcta ctaacttact gaatcctaag
    83821 ggatacggct taaagccttc cgatagtttt actcactgta accaacactg tgactaactt
    83881 tgttgaagta gtgagcagac ctaaggtctc agtacatgct caattcaaga acctaccttt
    83941 atcagcgtaa cggtgtcttt tacatccgtc tcaggatgaa gaccactggt cgcctgaccg
    84001 cttcgcttcc ttctcacaac cggtataaac tagcgtcagt atcactacgg actaaggaca
    84061 gacgtaccgc tatggctcac tcacggcaca tcaagtcagc acttaaagca atacacgcag
    84121 acaaccctag cgcatcatat gaggaactac gggagcacct gaagaccatt gttgagtggg
    84181 aacttggtgt cagccgtgat gacctgaacg acccggagtc ctaccagctt tacgttgacc
    84241 agtacgacga catcaagtcc aaccttcggg aagctgtagc gactgagcgt ctgactgtag
    84301 accagcatcg ctatatcaat gacgttatcg gtgtacttaa agcctgtcag gatagactca
    84361 agggcgataa ctctggcctg ttgtcttacc ttgagcctga gactggcagt ctaagaccgt
    84421 ccgtctccct atctgtatca tcggagcctg aagtccctga acctaaagca ctgaccctcg
    84481 cgtctctcat tgagcagtac gagcaggaga acgcccagaa ctggaagcca gcgaccctaa
    84541 gcgaaaaccg agcgtcacac tctacgctaa tcgagatatt cgactatctg gatattcagg
    84601 atgtcggcaa ggcgacccgt gcagatatgc ttagggtccg tgaagtcctc caacagctac
    84661 cgaagaaccg caagcaacgc tttaagtcta tgccgctgtc tgacctgctg aaccgggagt
    84721 ctaagactga ctgtcttgat gtcgttacca tcaacaataa atatcttatc aagatggctg
    84781 ctgtctttaa gtgggcggta cgtaacgacc tgatagctaa gaacctgacc gaaggtcttg
    84841 agctgaaagt cccgcagcgt aaagcctccg acgctcgtga tgctttcagt cctgagcagg
    84901 tagggcaact actggtcgcc gctaaagcgt actctcagaa gaccgcaggt aagccatacc
    84961 attattacgt caccgctctg gctgcgatta caggtgcgag acttaacgag gtggcccagc
    85021 ttcaggtcaa agacgtcagg gctactgagg ccgggaccgt atatatccat atcaacgaag
    85081 acgatagcag cctaccgggt aaaagtatta agaacgctca tagcgaccgc tgtgtcccgc
    85141 tggtcgatgg cgcttatggt tttgtattgg ctgactttat gtcgcttgtg gaagaccgta
    85201 gaaagactgc gggtgataat gctatggtat tcgatggcct caagctgatg aagaatggct
    85261 acggtgagca ggtgagcaaa tggtttaaca gaacgttgct gcctaaagtc cttgctgacc
    85321 gtagcggctt agcctttcac tcattcaggc acactgttgc gacccagtta aaacaacatg
    85381 gggtcgagtt agcgtatgct caggcgatta tgggtcatag ctcagggtct ataacttacg
    85441 accgttacgc taaagaggtc gaggtggata gattggttaa tgtgatggca ggtgtttata
    85501 aggagactgg attaaatggc tgagtattat ccggcggtgt ttgaagcaga ggcttttaac
    85561 tgtcctcact gtggtgtata tgcccgtcag ttctggcgta ggatgtatgg aagtgcgcgt
    85621 gagttggcgc aaaggacaac cgagttccga atgtcaacat gctctcattg cggtgatgat
    85681 gcttattggt atgacggcaa tatgattatt ccggcggctg gcaatgttga actaccaaat
    85741 ccagatatgc cagataactg caagtccgac tatatggaag cccgaagcat cattaatctg
    85801 tctcctaaag gggccgctgc actccttcgt ctttgccttc agaagctaat ggtccactta
    85861 ggcgagccgg gagaaaacat taataaagac atccggtcac tcgtccagaa aggactacct
    85921 gtacgcatcc agcaggcggc agatatctgt cgtattgtcg gcaatcaggc ggtccatccc
    85981 ggagagatta gtcttgatga tgacccgcag ttggcacacg gtctctttaa gctgctgaac
    86041 attattgtcg atgaccgaat cacgagaccg aaagaaattg aggctatgtt ccagagtatg
    86101 cctgaaggtc cacgtcaagg tattgagaat caggacagac aggcgaggga gcagcagcag
    86161 gccgctaacg aatgaaaccc acactatagg ggaagggggg ggactaaact atcactatag
    86221 gggagagacc ctaagtcccc ccgggcttaa tacagccacc gacccacaca ttcattaagt
    86281 ctcaggcttc cggtagcgga ccgattggaa accatccgca ataagcctag tgttaaatca
    86341 ccttggcctg agaccattcc ctttaacgta gcaatctctc tcctttgaca ggcggagaac
    86401 atcagaccgc agcggaagta tccgtctatg cgaccatcgg tgtgcgagat tgtgaagaac
    86461 caagacatca ttgctgaggt ctgccatcca tttgtggaca ggcggaaact caaaaaggtt
    86521 agaccagtgg tgtctgaggt tggagctttg agcctgacca gcaggcttga ggagaccatg
    86581 agttcatctt gttatggatt gtgttatcac aattccaata agaagaactt taagtccagc
    86641 ctccagtcct gtcagcccga agtatactac ggtcgctatg atgtggttct ccatacttcg
    86701 ggtctaactg gaagtactga acactttcgc gtcaggttct ccgaagttca ttcctgtttg
    86761 actaaactat cactataggg accatatctc gcctctgacc tcctgagaga cggcaagcga
    86821 gaaaccgtgg tccacgctag cttgctagcc accagcctct ggtcagcctt tgtgttcttt
    86881 ggtctcgtga cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc
    86941 tatggtcatc gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg
    87001 cctgctggtc tccctttacg tgggggcttg agtaggtcag tgggggtgcg acttcagggt
    87061 gcaaacaaaa ggagtacacg taaatgtctc aacaagaaat cagcgtcatt aacctcgacc
    87121 agcttgtctc catgacctca gttgaaattg ctgagctgac aggcaaggag caccgaaatg
    87181 tcctacgtga tattcgcaat atggctgagg agcttaacgc gctcaaaact gagcagtgct
    87241 caaaactgag cagtcatatc ggggtgactg aagatgtcta tctaaacgcg caggggaaac
    87301 agcagcctct gtaccgtctc gaccgtaagc atactttcat tctggtcgct ggctattccg
    87361 ttcacctccg cgccaagtgt tacgaccata ttcagacgtt agagcgtcga gtccttcaac
    87421 tggaagacca gaagaagcga gcagccattc agtccgctaa ccgccgaggc gtgacttggg
    87481 gcgactactg caaaacctat ggtctacccg ctcagaagct aatgaccgca cttcttcagc
    87541 atcgtggtct ctttcgtaag aacccaatca gtaacgagtg gtctgtaaat ccaaagtatt
    87601 ccgactgctt ccgtatcatt aagccgtcag accagaagtt ctcagcaggt ggttataact
    87661 ttcgcttcaa cgctaaggga ttggaggtct tcggtaagcc tgcgatagtc gataaactgc
    87721 gaggcatcct tattgccttc actggcaccg accagcagaa gcaagagcat ctcctgaagc
    87781 aagcccaatc cggcaagctg gagggactct aagatgattg acccgcgcac acctattggt
    87841 cgtgcaactc tccgctaccg tggtctgcct acccgtcacc tcctgtccct gctgagactt
    87901 ggagtcgata accctgatcg cccttactac agtcgagacg agctgatagc gatgctggtc
    87961 gaccgtgatt tgaataatca gttacgtcga gcctttgcga aactggagtc ttaacatctc
    88021 cggaaatttc cagaccttaa ccaaccttat ttaaaacctt ttgagatgac cgatgcgggg
    88081 agacccgtag gcattactgt gtctgcgata ccgatagtct caactctcaa gaggagtaat
    88141 tactatgtct a
//
LOCUS       DAZFJF010000007        81829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFJF010000007 DAZFJF010000000
VERSION     DAZFJF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.007"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..183
                     /gene="ydaE"
                     /locus_tag="ACHKAU_000531"
     CDS             13..183
                     /gene="ydaE"
                     /locus_tag="ACHKAU_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO5529660.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            258..533
                     /gene="racC"
                     /locus_tag="ACHKAU_000532"
     CDS             258..533
                     /gene="racC"
                     /locus_tag="ACHKAU_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HFO5529661.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            635..3235
                     /gene="recE"
                     /locus_tag="ACHKAU_000533"
     CDS             635..3235
                     /gene="recE"
                     /locus_tag="ACHKAU_000533"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO5529662.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDYEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDRISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            3228..4037
                     /gene="recT"
                     /locus_tag="ACHKAU_000534"
     CDS             3228..4037
                     /gene="recT"
                     /locus_tag="ACHKAU_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO5529663.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4093..4242
                     /gene="ralR"
                     /locus_tag="ACHKAU_000535"
     CDS             4093..4242
                     /gene="ralR"
                     /locus_tag="ACHKAU_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO5529664.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            4280..4468
                     /locus_tag="ACHKAU_000536"
     CDS             4280..4468
                     /locus_tag="ACHKAU_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO5529665.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            4568..4783
                     /gene="xisR"
                     /locus_tag="ACHKAU_000537"
     CDS             4568..4783
                     /gene="xisR"
                     /locus_tag="ACHKAU_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO5529666.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            4785..6020
                     /gene="intR"
                     /locus_tag="ACHKAU_000538"
     CDS             4785..6020
                     /gene="intR"
                     /locus_tag="ACHKAU_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5529667.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTLISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNMWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            6072..7007
                     /gene="ttcA"
                     /locus_tag="ACHKAU_000539"
     CDS             6072..7007
                     /gene="ttcA"
                     /locus_tag="ACHKAU_000539"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO5529668.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7136..8509)
                     /gene="dbpA"
                     /locus_tag="ACHKAU_000540"
     CDS             complement(7136..8509)
                     /gene="dbpA"
                     /locus_tag="ACHKAU_000540"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO5529669.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(8539..8712)
                     /gene="ynaL"
                     /locus_tag="ACHKAU_000541"
     CDS             complement(8539..8712)
                     /gene="ynaL"
                     /locus_tag="ACHKAU_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO5529670.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(8987..9970)
                     /gene="zntB"
                     /locus_tag="ACHKAU_000542"
     CDS             complement(8987..9970)
                     /gene="zntB"
                     /locus_tag="ACHKAU_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO5529671.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(9984..10031)
                     /gene="ydaN"
                     /locus_tag="ACHKAU_000543"
     CDS             complement(9984..10031)
                     /gene="ydaN"
                     /locus_tag="ACHKAU_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO5529672.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10225..11457
                     /gene="dgcM"
                     /locus_tag="ACHKAU_000544"
     CDS             10225..11457
                     /gene="dgcM"
                     /locus_tag="ACHKAU_000544"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO5529673.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(11478..12041)
                     /gene="smrA"
                     /locus_tag="ACHKAU_000545"
     CDS             complement(11478..12041)
                     /gene="smrA"
                     /locus_tag="ACHKAU_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO5529674.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12371..13279)
                     /gene="abgR"
                     /locus_tag="ACHKAU_000546"
     CDS             complement(12371..13279)
                     /gene="abgR"
                     /locus_tag="ACHKAU_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5529675.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            13455..14765
                     /gene="abgA"
                     /locus_tag="ACHKAU_000547"
     CDS             13455..14765
                     /gene="abgA"
                     /locus_tag="ACHKAU_000547"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO5529676.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            14765..16210
                     /gene="abgB"
                     /locus_tag="ACHKAU_000548"
     CDS             14765..16210
                     /gene="abgB"
                     /locus_tag="ACHKAU_000548"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO5529677.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            16247..17773
                     /gene="abgT"
                     /locus_tag="ACHKAU_000549"
     CDS             16247..17773
                     /gene="abgT"
                     /locus_tag="ACHKAU_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO5529678.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            17784..18299
                     /gene="ogt"
                     /locus_tag="ACHKAU_000550"
     CDS             17784..18299
                     /gene="ogt"
                     /locus_tag="ACHKAU_000550"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO5529679.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            18494..19246
                     /gene="fnr"
                     /locus_tag="ACHKAU_000551"
     CDS             18494..19246
                     /gene="fnr"
                     /locus_tag="ACHKAU_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO5529680.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            19398..20348
                     /gene="uspE"
                     /locus_tag="ACHKAU_000552"
     CDS             19398..20348
                     /gene="uspE"
                     /locus_tag="ACHKAU_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO5529681.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(20398..20655)
                     /gene="ynaJ"
                     /locus_tag="ACHKAU_000553"
     CDS             complement(20398..20655)
                     /gene="ynaJ"
                     /locus_tag="ACHKAU_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO5529682.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            20899..21930
                     /gene="ynaI"
                     /locus_tag="ACHKAU_000554"
     CDS             20899..21930
                     /gene="ynaI"
                     /locus_tag="ACHKAU_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO5529683.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(21981..23594)
                     /gene="mppA"
                     /locus_tag="ACHKAU_000555"
     CDS             complement(21981..23594)
                     /gene="mppA"
                     /locus_tag="ACHKAU_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO5529684.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(23931..24830)
                     /gene="pgrR"
                     /locus_tag="ACHKAU_000556"
     CDS             complement(23931..24830)
                     /gene="pgrR"
                     /locus_tag="ACHKAU_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO5529685.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            24968..25888
                     /locus_tag="ACHKAU_000557"
     CDS             24968..25888
                     /locus_tag="ACHKAU_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5529686.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            25888..26820
                     /locus_tag="ACHKAU_000558"
     CDS             25888..26820
                     /locus_tag="ACHKAU_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO5529687.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            26838..27476
                     /locus_tag="ACHKAU_000559"
     CDS             26838..27476
                     /locus_tag="ACHKAU_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5529688.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            27785..28513
                     /gene="mpaA"
                     /locus_tag="ACHKAU_000560"
     CDS             27785..28513
                     /gene="mpaA"
                     /locus_tag="ACHKAU_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO5529689.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(28488..29453)
                     /gene="ycjG"
                     /locus_tag="ACHKAU_000561"
     CDS             complement(28488..29453)
                     /gene="ycjG"
                     /locus_tag="ACHKAU_000561"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO5529690.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            29572..30078
                     /gene="tpx"
                     /locus_tag="ACHKAU_000562"
     CDS             29572..30078
                     /gene="tpx"
                     /locus_tag="ACHKAU_000562"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO5529691.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(30122..31663)
                     /gene="tyrR"
                     /locus_tag="ACHKAU_000563"
     CDS             complement(30122..31663)
                     /gene="tyrR"
                     /locus_tag="ACHKAU_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO5529692.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(31811..32872)
                     /gene="ycjF"
                     /locus_tag="ACHKAU_000564"
     CDS             complement(31811..32872)
                     /gene="ycjF"
                     /locus_tag="ACHKAU_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO5529693.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDGKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(32869..34266)
                     /gene="ycjX"
                     /locus_tag="ACHKAU_000565"
     CDS             complement(32869..34266)
                     /gene="ycjX"
                     /locus_tag="ACHKAU_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO5529694.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            34421..35419
                     /gene="ycjW"
                     /locus_tag="ACHKAU_000566"
     CDS             34421..35419
                     /gene="ycjW"
                     /locus_tag="ACHKAU_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5529695.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(35530..36435)
                     /gene="ompG"
                     /locus_tag="ACHKAU_000567"
     CDS             complement(35530..36435)
                     /gene="ompG"
                     /locus_tag="ACHKAU_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO5529696.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(36480..37562)
                     /locus_tag="ACHKAU_000568"
     CDS             complement(36480..37562)
                     /locus_tag="ACHKAU_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5529697.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(37576..38235)
                     /gene="pgmB"
                     /locus_tag="ACHKAU_000569"
     CDS             complement(37576..38235)
                     /gene="pgmB"
                     /locus_tag="ACHKAU_000569"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO5529698.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(38232..40499)
                     /gene="ycjT"
                     /locus_tag="ACHKAU_000570"
     CDS             complement(38232..40499)
                     /gene="ycjT"
                     /locus_tag="ACHKAU_000570"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO5529699.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(40496..41551)
                     /gene="ycjS"
                     /locus_tag="ACHKAU_000571"
     CDS             complement(40496..41551)
                     /gene="ycjS"
                     /locus_tag="ACHKAU_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5529700.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(41561..42349)
                     /gene="ycjR"
                     /locus_tag="ACHKAU_000572"
     CDS             complement(41561..42349)
                     /gene="ycjR"
                     /locus_tag="ACHKAU_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5529701.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            complement(42367..43419)
                     /gene="ycjQ"
                     /locus_tag="ACHKAU_000573"
     CDS             complement(42367..43419)
                     /gene="ycjQ"
                     /locus_tag="ACHKAU_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO5529702.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(43450..44292)
                     /gene="ycjP"
                     /locus_tag="ACHKAU_000574"
     CDS             complement(43450..44292)
                     /gene="ycjP"
                     /locus_tag="ACHKAU_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5529703.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(44279..45160)
                     /gene="ycjO"
                     /locus_tag="ACHKAU_000575"
     CDS             complement(44279..45160)
                     /gene="ycjO"
                     /locus_tag="ACHKAU_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5529704.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(45181..46473)
                     /gene="ycjN"
                     /locus_tag="ACHKAU_000576"
     CDS             complement(45181..46473)
                     /gene="ycjN"
                     /locus_tag="ACHKAU_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5529705.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(46487..48166)
                     /gene="ycjM"
                     /locus_tag="ACHKAU_000577"
                     /pseudo
     CDS             complement(46487..48166)
                     /gene="ycjM"
                     /locus_tag="ACHKAU_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(48378..48692)
                     /gene="pspE"
                     /locus_tag="ACHKAU_000578"
     CDS             complement(48378..48692)
                     /gene="pspE"
                     /locus_tag="ACHKAU_000578"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO5529706.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(48767..48988)
                     /gene="pspD"
                     /locus_tag="ACHKAU_000579"
     CDS             complement(48767..48988)
                     /gene="pspD"
                     /locus_tag="ACHKAU_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO5529707.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(48997..49356)
                     /gene="pspC"
                     /locus_tag="ACHKAU_000580"
     CDS             complement(48997..49356)
                     /gene="pspC"
                     /locus_tag="ACHKAU_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO5529708.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(49356..49580)
                     /gene="pspB"
                     /locus_tag="ACHKAU_000581"
     CDS             complement(49356..49580)
                     /gene="pspB"
                     /locus_tag="ACHKAU_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO5529709.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(49634..50302)
                     /gene="pspA"
                     /locus_tag="ACHKAU_000582"
     CDS             complement(49634..50302)
                     /gene="pspA"
                     /locus_tag="ACHKAU_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO5529710.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            50469..51446
                     /gene="pspF"
                     /locus_tag="ACHKAU_000583"
     CDS             50469..51446
                     /gene="pspF"
                     /locus_tag="ACHKAU_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO5529711.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(51566..52831)
                     /gene="puuE"
                     /locus_tag="ACHKAU_000584"
     CDS             complement(51566..52831)
                     /gene="puuE"
                     /locus_tag="ACHKAU_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO5529712.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(52869..54149)
                     /gene="puuB"
                     /locus_tag="ACHKAU_000585"
     CDS             complement(52869..54149)
                     /gene="puuB"
                     /locus_tag="ACHKAU_000585"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO5529713.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(54151..55638)
                     /gene="puuC"
                     /locus_tag="ACHKAU_000586"
     CDS             complement(54151..55638)
                     /gene="puuC"
                     /locus_tag="ACHKAU_000586"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO5529714.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(55913..56470)
                     /gene="puuR"
                     /locus_tag="ACHKAU_000587"
     CDS             complement(55913..56470)
                     /gene="puuR"
                     /locus_tag="ACHKAU_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO5529715.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(56497..57261)
                     /gene="puuD"
                     /locus_tag="ACHKAU_000588"
     CDS             complement(56497..57261)
                     /gene="puuD"
                     /locus_tag="ACHKAU_000588"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO5529716.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            57473..58891
                     /gene="puuA"
                     /locus_tag="ACHKAU_000589"
     CDS             57473..58891
                     /gene="puuA"
                     /locus_tag="ACHKAU_000589"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO5529717.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            59040..59204
                     /locus_tag="ACHKAU_000590"
     CDS             59040..59204
                     /locus_tag="ACHKAU_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529718.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            59194..60579
                     /gene="puuP"
                     /locus_tag="ACHKAU_000591"
     CDS             59194..60579
                     /gene="puuP"
                     /locus_tag="ACHKAU_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO5529719.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            60713..60958
                     /gene="ymjA"
                     /locus_tag="ACHKAU_000592"
     CDS             60713..60958
                     /gene="ymjA"
                     /locus_tag="ACHKAU_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO5529720.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            61271..62914
                     /gene="sapA"
                     /locus_tag="ACHKAU_000593"
     CDS             61271..62914
                     /gene="sapA"
                     /locus_tag="ACHKAU_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO5529721.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            62911..63876
                     /gene="sapB"
                     /locus_tag="ACHKAU_000594"
     CDS             62911..63876
                     /gene="sapB"
                     /locus_tag="ACHKAU_000594"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO5529722.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            63863..64753
                     /gene="sapC"
                     /locus_tag="ACHKAU_000595"
     CDS             63863..64753
                     /gene="sapC"
                     /locus_tag="ACHKAU_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO5529723.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            64753..65745
                     /gene="sapD"
                     /locus_tag="ACHKAU_000596"
     CDS             64753..65745
                     /gene="sapD"
                     /locus_tag="ACHKAU_000596"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO5529724.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            65747..66553
                     /gene="sapF"
                     /locus_tag="ACHKAU_000597"
     CDS             65747..66553
                     /gene="sapF"
                     /locus_tag="ACHKAU_000597"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO5529725.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            66621..66974
                     /gene="ycjD"
                     /locus_tag="ACHKAU_000598"
     CDS             66621..66974
                     /gene="ycjD"
                     /locus_tag="ACHKAU_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO5529726.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            67342..68130
                     /gene="fabI"
                     /locus_tag="ACHKAU_000599"
     CDS             67342..68130
                     /gene="fabI"
                     /locus_tag="ACHKAU_000599"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO5529727.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            68275..69402
                     /gene="yciW"
                     /locus_tag="ACHKAU_000600"
     CDS             68275..69402
                     /gene="yciW"
                     /locus_tag="ACHKAU_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO5529728.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            69470..71404
                     /gene="rnb"
                     /locus_tag="ACHKAU_000601"
     CDS             69470..71404
                     /gene="rnb"
                     /locus_tag="ACHKAU_000601"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO5529729.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLIALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(71482..71586)
                     /locus_tag="ACHKAU_000602"
     CDS             complement(71482..71586)
                     /locus_tag="ACHKAU_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529730.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            71640..73625
                     /gene="pdeR"
                     /locus_tag="ACHKAU_000603"
     CDS             71640..73625
                     /gene="pdeR"
                     /locus_tag="ACHKAU_000603"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5529731.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(73607..73735)
                     /gene="ymiD"
                     /locus_tag="ACHKAU_000604"
     CDS             complement(73607..73735)
                     /gene="ymiD"
                     /locus_tag="ACHKAU_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO5529732.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            73773..73946
                     /gene="yciZ"
                     /locus_tag="ACHKAU_000605"
     CDS             73773..73946
                     /gene="yciZ"
                     /locus_tag="ACHKAU_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO5529733.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            74036..74785
                     /gene="yciT"
                     /locus_tag="ACHKAU_000606"
     CDS             74036..74785
                     /gene="yciT"
                     /locus_tag="ACHKAU_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO5529734.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            75054..75272
                     /gene="osmB"
                     /locus_tag="ACHKAU_000607"
     CDS             75054..75272
                     /gene="osmB"
                     /locus_tag="ACHKAU_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO5529735.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(75398..75724)
                     /gene="yciH"
                     /locus_tag="ACHKAU_000608"
     CDS             complement(75398..75724)
                     /gene="yciH"
                     /locus_tag="ACHKAU_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO5529736.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(75724..76461)
                     /gene="pyrF"
                     /locus_tag="ACHKAU_000609"
     CDS             complement(75724..76461)
                     /gene="pyrF"
                     /locus_tag="ACHKAU_000609"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO5529737.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(76653..77822)
                     /gene="lapB"
                     /locus_tag="ACHKAU_000610"
     CDS             complement(76653..77822)
                     /gene="lapB"
                     /locus_tag="ACHKAU_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO5529738.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(77829..78137)
                     /gene="lapA"
                     /locus_tag="ACHKAU_000611"
     CDS             complement(77829..78137)
                     /gene="lapA"
                     /locus_tag="ACHKAU_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO5529739.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(78286..79050)
                     /gene="pgpB"
                     /locus_tag="ACHKAU_000612"
     CDS             complement(78286..79050)
                     /gene="pgpB"
                     /locus_tag="ACHKAU_000612"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO5529740.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            79220..79810
                     /gene="ribA"
                     /locus_tag="ACHKAU_000613"
     CDS             79220..79810
                     /gene="ribA"
                     /locus_tag="ACHKAU_000613"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO5529741.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(79874..>81829)
                     /gene="acnA"
                     /locus_tag="ACHKAU_000614"
     CDS             complement(79874..>81829)
                     /gene="acnA"
                     /locus_tag="ACHKAU_000614"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO5529742.1"
                     /translation="PVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKF
                     VEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEK
                     YAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELE
                     VNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLG
                     LKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIE
                     TAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEPIG
                     HDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYG
                     WQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAG
                     RYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVV
                     SIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNL
                     IGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCR
                     CRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        20369 a        21060 c        20156 g        20244 t
ORIGIN      
        1 ggagtcatgg tgatgactaa gaaaataaaa tgtgcttacc acctttgcaa aaaagacgtt
       61 gaagaaagca aagctattga aagaatgctt cacttcatgc acgggatttt atcaaaagac
      121 gaaccgagaa aatattgcag tgaagcttgt gccgaaaaag accagatggc acatgaactt
      181 taattaattg actattcgaa actgaattta tgccagaaat ggcaggtatt cgctcaacct
      241 taattaagga gaaaaacatg attaccaatt atgaagccac tgttgtaact accgatgaca
      301 ttgttcacga ggtgaatctg gaaggaaagc gcattggcta cgtaattaaa acagaaaata
      361 aagaaacccc attcactgtg gttgatatcg atggtccatc aggcaacgta aaaacacttg
      421 atgaaggtgt caaaaaaatg tgcctggtgc atatcggaaa gaatctgccc gcagaaaaaa
      481 aagccgaatt tctggcaact ctaattgcaa tgaaattaaa aggtgaaatc tgaaagaaat
      541 agcctgcgta tggcgcaggc tatgaacagt gtgtatccgg caagatcatt cactgaacaa
      601 aacgaatttt aatctgagtt gaggttaaaa aacaatgagc acaaaaccac tcttcctgtt
      661 acggaaagcg aaaaaatcat ccggtgaacc tgacgtcgtc ctgtgggcaa gcaacgattt
      721 tgaatcgacc tgtgccactc tggactacct gatcgttaag tcaggtaaaa aactgagcag
      781 ctattttaaa gctgttgcca cgaattttcc tgtcgttaat gacctgcccg ctgaaggtga
      841 gatcgatttt acctggagtg aacgctatca actcagcaaa gactccatga catgggaact
      901 aaaaccggga gcagcaccag acaacgctca ctatcaaggc aataccaacg tcaacggcga
      961 agacatgact gagattgagg agaatatgct actcccaatt tctggccagg aactgcccat
     1021 tcgttggctt gctcaacacg gcagcgaaaa accggtaacg cacgtttcac gcgacggact
     1081 ccaggcatta cacattgctc gggctgaaga actaccggct gttactgccc tggctgtttc
     1141 ccacaaaacc agcctgctcg acccgctgga aattcgcgaa ctccacaaac tggttcgtga
     1201 cactgacaaa gttttcccta atcctggtaa ttcaaacctg ggactgataa ctgctttttt
     1261 cgaagcatac ctgaacgctg actacaccga tcgaggactg ctgacaaaag agtggatgaa
     1321 gggtaatcgt gtttcacaca tcactcgcac ggcttccggt gctaatgctg gcggcggaaa
     1381 cctcaccgat cgcggcgaag gtttcgtaca cgatctgacg tcactggcgc gggacgtagc
     1441 cactggcgta ctggcccgtt caatggatct ggacatctat aaccttcatc cggcacacgc
     1501 taaacgcatt gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1561 attcatcacc atgcctggcg ggctggatta ttcccgcgcc atcgtggttg cgtccgtaaa
     1621 agaagcacca attgggatcg aggtcatccc cgcgcacgtc actgaatatc tgaacaaagt
     1681 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     1741 ctcctctgcc ccgatgccgc agcgagtaac agaagaagga aaacaggatt atgaagaaaa
     1801 accgcaacca tctggaacaa cggcagttga acagggagag gctgaaacaa tggaaccgga
     1861 cgcaactgaa catcatcagg acacgcagcc gctggatgct cagtcacagg taaattctgt
     1921 tgatgcgaaa tatcaggaac tgcgggcaga actccatgaa gcccggaaaa acattccatc
     1981 aaaaaatcct gtcgatgccg ataaattgct tgctgcatca cgtggtgaat ttgttgacag
     2041 aattagcgac ccgaacgatc cgaaatgggt aaaggggatc cagactcgcg attgtgtgta
     2101 ccagaaccag ccagaaacgg aaaaaaccag cccggatatg aatcaacctg agccagtagt
     2161 gcaacaggaa ccggaaatag cctgcaatgc ctgcggccag actggcgggg ataactgccc
     2221 tgactgtggt gcggtgatgg gcgacgcaac ataccaggaa acattcgatg aagagagtca
     2281 ggttgaagct aaggaaaatg atccggagga aatggaaggc gctgaacatc cgcacaatga
     2341 gaatgctggc agcgatccgc atcgcgattg cagtgatgaa actggcgaag tcgcagatcc
     2401 cgtaatcgta gaagacatag agccaggtat ttattacgga atttcgaatg agaattacca
     2461 cgcgggtccc ggtgtcagta agtctcagct cgatgacatt gctgatactc cggcactgta
     2521 tttgtggcgt aaaaatgccc ccgtggacac cacaaagaca aaaacgctcg atttaggaac
     2581 cgctttccac tgccgggtac ttgagccgga agaattcagt aaccgcttta tcgtagcacc
     2641 tgaatttaac cgccggacaa actccggaaa agaagaagag aaagcgtttc tgagggaatg
     2701 cgcaagcaca ggaaaaacgg ttatcactgc cgaagaaggc cggaaaattg aactcatgta
     2761 tcagagcgtt atggctttgc cgctggggca atggcttgtt gaaagcgccg gacacgctga
     2821 atcatcaatt tactgggaag atcctgaaac agcaattttg tgtcggtgcc gtccggacaa
     2881 aattatccct gaatttcact ggatcatgga cgtgaaaact acggcggata ttcaacgatt
     2941 caaaaccgct tattacgact accgctatca cgttcaggat gcattctaca gtgacggtta
     3001 tgaagcacag tttggagtgc agccaacttt cgtttttctg gttgccagca caactattga
     3061 atgcggacgt tatccggttg aaattttcat gatgggcgaa gaagcaaaac tggcaggtca
     3121 gctggaatat caccgcaatc tgcgaaccct ggctgactgc ctcaataccg atgaatggcc
     3181 agctattaag acgttatcac tgccccgctg ggctaaggaa tatgcaaatg actaagcaac
     3241 caccaatcgc aaaagccgat ctgcaaaaaa ctcagggaaa ccgtgcacca gcagcaatta
     3301 aaaataacga cgtgattagt tttattaacc agccatcaat gaaagagcaa ctggcagcag
     3361 ctcttccacg ccatatgacg gctgaacgta tgatccgtat cgccaccaca gaaattcgta
     3421 aagttccggc gttaggaaac tgtgacacta tgagttttgt cagtgcaatc gtacagtgtt
     3481 cacagctcgg acttgagccc ggtagcgccc tcggtcatgc atatttactg ccttttggta
     3541 ataaaaacga aaagagcggt aaaaaaaacg ttcagctaat cattggctat cgcggcatga
     3601 ttgatctggc tcgccgttca ggtcaaatcg ccagcctgtc agcccgtgtt gtccgtgaag
     3661 gtgacgagtt taatttcgaa tttggccttg atgaaaagtt aatacaccgc ccaggagaaa
     3721 acgaagatgc ccctgttacc cacgtctatg ctgtcgcaag actgaaagac ggaggtactc
     3781 agtttgaagt tatgacgcgc aaacagattg agctggtgcg cagccagagt aaagctggta
     3841 ataacgggcc gtgggtaact cactgggaag aaatggcaaa gaaaacggct attcgtcgcc
     3901 tgttcaaata tctgcccgta tcaattgaga tccagcgtgc agtatcaatg gatgaaaagg
     3961 aaccactgac aatcgatcct gcagattcct ctgtattaac cggggaatac agtgtaatcg
     4021 ataattcaga ggaataattc agcctggcgg tgtaatgcac cgccaacttg aaatattttt
     4081 atgagaaaaa ttatgagata tgacaatgtt aaaccatgtc cattttgtgg ttgtccatca
     4141 gtaacggtga aagccatttc aggatattac cgagcgaagt gtaacggatg cgaatcccga
     4201 accggttatg gtggaagtga aaaagaagca ctcgaacgat gaaataaacg aaccactgga
     4261 aataataatg gaggtgttca tgtataaaat taccgccact attgaaaagg aaggtggcac
     4321 tcctactaac tggacaagat attcaaaatc taaactaacg aaatcagaat gcgaaaaaat
     4381 gctctcaggt aaaaaagaag caggcgtttc cagagagcag aaagtaaaac tgataaattt
     4441 taattgcgag aaacttcagt cctcgtgaat tgcattgtat tcaaattaaa acttcatagc
     4501 tgattattaa taatcaacat cgggcgtcaa tttcagtcta acattggcgc ctgccagagg
     4561 tgatgcgatg gcacaagtaa tctttaatga agagtggatg gttgaatacg gcctgatgct
     4621 tcgcactggt ctgggggcca gacaaattga agcataccgc cagaactgtt gggtggaggg
     4681 cttccacttc aaacgagtat ctcctttagg taagccagac agcaaacgag ggattatctg
     4741 gtacaactat ccaaagataa atcagtttat caaagactca tgatatgtct aaattaccaa
     4801 caggtgtcga gattagaggt agatacattc gcatctggtt catgtttcga ggaaaacgat
     4861 gtcgggaaac attaaaaggc tgggagatta caaacagtaa tattaaaaag gccggaaatt
     4921 taagagcgct gatagttcat gaaataaact ccggtgaatt tgagtattta agacgttttc
     4981 cccagtccag cactggggca aaaatggtga caacgagagt cataaaaacg ttcggagagc
     5041 tttgtgatat ctggacaaaa attaaagaga cagagttaac aacaaacaca atgaagaaaa
     5101 cgaaatcaca attaaaaaca ctcagaataa taatttgtga aagtaccctg atatcacata
     5161 ttcgttatag cgatatctta aactaccgga atgaactgct gcatggagaa acgctttacc
     5221 tggataatcc aagatccaac aaaaaaggaa gaaccgtgcg cacagttgat aactatatcg
     5281 ccctgctctg ttcgctgtta cgttttgcgt atcagtcggg atttatatca accaaaccat
     5341 ttgaaggagt aaaaaaatta cagcgaaaca gaataaagcc tgatccgtta tctaaaacag
     5401 aattcaatgc attaatggaa agtgaaaaag gacagagcca gaacatgtgg aaatttgcag
     5461 tttactcagg acttcgtcac ggggaactgg cagctctggc gtgggaggat gtggatctcg
     5521 aaaagggaat agtgaatgtc agaagaaacc tgacgatact tgatatgttc ggtcccccaa
     5581 aaacaaatgc cgggatccgg acagtaacac tactgcagcc tgctcttgaa gcactgaagg
     5641 agcaatacaa actgaccggg catcatcgca aaagcgaaat caccttttat catcgggagt
     5701 acggcagaac cgaaaagcaa aaactgcatt ttgttttcat gcccagggtg tgtaacggaa
     5761 aacaaaaacc ttattactcg gtaagcagtt tgggggcaag gtggaatgca gcagtaaaac
     5821 gtgctggtat tcgccgccgt aatccgtacc atacgcggca tacttttgcc tgctggctgt
     5881 tgacggcagg agcgaacccg gcatttatag ccagccaaat ggggcatgaa actgcgcaga
     5941 tggtgtatga aatttacggt atgtggattg atgacatgaa cgacgaacag atagccatgt
     6001 tgaatgcgcg gttatcatag ttgcaaagtt tgcccccaat ttgccccatt tagtaccaga
     6061 gaactgaaat aatgcaagaa aatcaacaaa ttacaaagaa agaacaatac aacctgaaca
     6121 aattacaaaa acgtctgcgt cgtaacgtgg gcgaagccat tgctgacttc aatatgattg
     6181 aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa agacagctat accatgctgg
     6241 agattctgcg caatttgcag caaagcgcgc caatcaattt ttcgctggtg gctgttaacc
     6301 tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc cgagtatctt gaaaagctgg
     6361 gcgttgagta caagattgtt gaagagaata cttacggtat cgtgaaagag aagattccag
     6421 agggcaaaac cacttgctca ctgtgttctc gccttcgtcg cggtatcctt tatcgtaccg
     6481 caacggaact gggggcgacg aagatcgcgt tgggtcacca tcgtgacgat atcctgcaaa
     6541 cgttgttctt aaatatgttc tacggcggca agatgaaagg tatgcctccg aaactgatga
     6601 gcgatgatgg caaacatatc gttattcgtc cgctggccta ctgccgcgag aaagatattc
     6661 agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc ggttcacagc
     6721 ctaacctgca acgtcaggtg attgctgaca tgttgcgtga ctgggataaa cgttatccag
     6781 ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata
     6841 ccaacctgtt cgattttaaa ggcattaccc acggttctga agtggttaac gggggtgatc
     6901 tggcgtttga tcgcgaagag atcccactac aaccggcggg ctggcagcca gaagaagatg
     6961 aaaatcagtt ggatgagtta cgactgaatg tggttgaagt gaaataacca ggatagcgcc
     7021 cgatgcgcaa gcgtatcggg ctactcttat ggaggccgga taagacgcgg ccagcgtcgc
     7081 atccggcaat cccgaataag atgtttactc ttgcacccgg caattcaaca tttcattatt
     7141 ttaataaccg cacccggcac gtttttcctt taatcttccc gccctgtaac tgtttccatg
     7201 ctttatgagc aacagcctga cggaccgcga catagacatg cgccggatgc acggcgattt
     7261 tgccaatatc tgcgccatca agcccgatat ctcctgtcag tgcacctaat acatcacccg
     7321 ggcgcatttt ggcttttttc ccgccatcga tacacaacgt tgccatttct gcttccagcg
     7381 gcacaatgga actattagct ggcggcgttt gccagttaag ttttatctgc aacatgtcag
     7441 aaatgatatt ggcccgctgt gcttcttccg gagcacagaa actgatcgcc agaccgctat
     7501 ttcctgcacg agctgtacga ccgatgcgat gtacatgaac ttcagggtcc cacgccagct
     7561 caaagttcac caccagctca agcgatttaa tatccagacc acgcgcagca acatcagtag
     7621 caaccagtac acgggcgcta ccgttagcaa aacgtaccag agtctgatcg cgatcgcgtt
     7681 gctccagatc gccgtgtaac gacaatgcac tttgccctac ttcattcagc gcgtcgcaga
     7741 cagcctggca atcttttttg gtattgcaaa acaccacgca agaggatggc tgatgcaagc
     7801 ttaataaccg ttgcaacaga gggattttgc ctttgctgga tgtctcataa aattgttgtt
     7861 caatgggtgg caaagcatct gttgagtcaa tttcaatcgc caaaggatcg cgttgtactc
     7921 gtccgctgat tgcggcgatg gcttccggcc aggttgccga aaacagaagc gtctgtcgag
     7981 atgcaggcgc aaaacggatg acatcatcaa tggcatcgct aaatcccata tccagcatgc
     8041 ggtcggcctc atccatcacc aaagtattca gcgcatccag tgataccgtg cctttttgca
     8101 agtgatccag caaacgaccc ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat
     8161 cacgctgcat accgaacggt tgaccaccgc acaacgtcaa aattttggta tttggcagaa
     8221 aacgcgccag ccgacgcaat tcacctgcca cctgatccgc cagttcacgc gtaggacaca
     8281 gcactaaagc ctgggtttga aatagcgacg catcaatttg ctgtaacaag ccgaggccaa
     8341 aagccgccgt tttgccgctg ccggttttcg cctgcacgcg aacatctttt ccggcaagga
     8401 tcgccggaag cgcggcggcc tgcaccggcg tcatggttaa ataacccaac tcattaaggt
     8461 tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa agcggtcaca atctattctc
     8521 gtggtcatcg acgcaaagtt agcaggcgcg tatcctcgca gatctacgct cacgatgcga
     8581 caatttaatc ggttcttcat cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg
     8641 atcgggcact ggaacaggat cgctaggaat cggttcaggg acaggaattt gcaaataaat
     8701 aagtgtcgtc atatttccct ccggtcattg ggtggactct taaagggtag acgctgataa
     8761 ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc gtacgaatca attgtgctat
     8821 gcagtaattc aaaaaaggaa gtaagacaat atggagcgca acgcccatcg cttgacgttg
     8881 cattcacctg caatggagat attgccctga atgggtagag agtttattga cttcgctcaa
     8941 actttgcggc gtttttgtat acagacagcc ggaaaaattg cttttgttac aaccatttac
     9001 tacggtgcaa ccataaagca acaccaccaa taagaacaac taacagaata caaaaaattg
     9061 aaaatccgaa ttgccacccg ccgccaggga tcccaccaag gttgacgcca aataaccctg
     9121 tcagaaaggt actgggtaaa aagaccattg ccatcaatga cattgtatag gtacgacgag
     9181 ctaaattttc ctgcatcacc tgagcgattt catccgccat cacaccagtc cgtgctatac
     9241 aggcgtcgat ttcgtcaagg ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc
     9301 gttggtcatc gctcatccac ggcaaacgtt cactggcaag acgagcatag acatcacgtt
     9361 gcggtgccat atagcgacgc atcacgatta attgtttgcg cagcagagcc aggaatccac
     9421 gcggtggaat ttgctgatca aggagattat cttcaaggtc gataatttta tcgtgcagct
     9481 gctcgataaa ttcgctggaa tgatcggtca acgcatcgca cacatccacc agccaccctc
     9541 cgcaatcggt cggacccgtg ccctcttcca gatcgctcac cacatcgtcc agcgccagca
     9601 ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat atatacacgc atggcgacca
     9661 gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg caatgtaata agcgtgcctt
     9721 caccgagacg gctgactcgg ggccgtgtgc tctcgcccgc cagcgcatca cgtacgttat
     9781 tgggaagcag cggtgttgtc gccagccatt gggcgctatc atggtgtaca taattaaggt
     9841 ggagccagca gggatgcgct tcatcaatca catctgtatt ttccagcggt ttaacgccgc
     9901 ctctaccatc cagcatccag gcaaatactg catccgggac attaacgtcc gatcccttaa
     9961 tcgcttccac agtgcctcca tcatcaacgc attattttgt agtctagcct tctggccctg
    10021 ttacgcaaca tctcatcacc ccattaccct gaaatgatta ataaaattct gtctaaattg
    10081 aatacaaaaa gcaaaatgct tttccgtata caaaccgtgt gaagtgttaa atagcgtcta
    10141 tcattatcag aattatctga tcatatgacg tggctttttt gcgatcggat agcaacaaaa
    10201 attgataaaa ataacgggat ctcaatgatt acgcacaact tcaataccct ggacttactc
    10261 accagtcctg tctggattgt ttcgcccttt gaggaacagt taatttatgc caatagcgcg
    10321 gcgcgactgt tgatgcaaga cctcacgttt agtcagctac gaaccggacc ctattccgtc
    10381 tcctcacaaa aagaactgcc gaaatacctc tccgatctgc aaaaccaaca cgatattatc
    10441 gaaatcctca ctgttcagcg taaagaagag gaaacagcat taagctgtcg gcttgttttg
    10501 cgagagctga cagaaacaga accggtgatt attttcgaag gtatcgaagc gccggcaacg
    10561 ctgggtttaa aagccagtcg ctcggcaaat tatcagcgca aaaaacaagg tttttatgcg
    10621 cgcttttttc tgactaactc tgcaccaatg ttgttgattg acccctcacg agatggccaa
    10681 atcgtcgatg ctaacctcgc cgcgctcaat ttctatggtt ataaccatga aacgatgtgc
    10741 cagaaacata cctgggaaat taatatgctc gggcgtcgcg tcatgcctat catgcatgaa
    10801 atttcgcatt tacccggcgg tcataaacct ttgaattttg ttcataaact ggcggatgga
    10861 tctactcgtc atgtgcagac ctatgccggg ccgattgaaa tttatggcga caagctcatg
    10921 ttatgtattg tgcatgatat tactgagcaa aaacggctgg aggagcagct ggaacatgct
    10981 gctcaccatg acgcgatgac cggattactg aatcggcgac agttttatca cattaccgag
    11041 ccaggccaaa tgcagcacct cgccatcgcc caggattaca gcttgttgct catcgacacc
    11101 gatcgtttta aacacattaa cgatctctat gggcattcta aaggtgatga ggtgttatgc
    11161 gccctcgccc gcaccctcga aagttgcgct cgcaaaggcg atttggtgtt tcgttgggga
    11221 ggcgaagagt ttgtcttatt gctaccaaga accccactgg ataccgcgct ttcgctggct
    11281 gaaactatcc gtgtaagcgt ggcaaaagtg agtatttcgg gcttaccacg ttttaccgtc
    11341 agcattggtg tggcgcatca cgaaggaaat gaaagcatcg atgaactgtt taaacgcgtt
    11401 gatgatgctt tgtatcgggc gaaaaatgat ggacgcaacc gcgtgctggc ggcataagcc
    11461 gcggatgcgt ctcgagatca acggctgcgc ttagcgtggc gctcccagtt ttcttgcttc
    11521 gcctgcgccg ttttacgtag tgcgacgtaa cacgccccgc tgccgccatg atgcggtagc
    11581 gcggtgcaat atgcctgaac atcatcaaat tcggtcagcc agcgcgccac atagctgcgg
    11641 acaatattgg catgcgattt atcatcccgt cctttgccat gaataatcag cacgttacgc
    11701 aaaccatcag ccatggcttg ttgaatgaaa ctgaacatca ttttgcggca ctcttccacc
    11761 ggctggcgca aaagattcag gctcgcctgt tgcggatatt taccactgcg cagcttatcc
    11821 agcaccccat gttgcaaccc ttcccgccga aactccagcg gctgacttag tgggatgatg
    11881 tcgagaaatc cggtggtgag gaaattatca agctgcagcg tgtcgatacg ctgcggcgca
    11941 cgttggttac gcgttggatg ccagtggaca tcggtagcac gtttcagcgg ctggacatct
    12001 tccatggcgt caagaaacag cgatttgtcg tcaaggttca tgttacatcc tcccgcaatt
    12061 aagagcgcga tatgataacc agaccgggtc ggtccaacaa cgtcttaccc aaaattccag
    12121 taataagttc caaatattgc cgatatttta agcaaaatgc ttatgcatga ttattcattc
    12181 acgatattaa taatgtaact tatattttcg tgaaatctgt cactgaagaa aattggcaac
    12241 taaaggttaa aaccgttata acacagtcac cggcgcagag gagacaatgc cggacttaag
    12301 acgcggatgc actgctgtgt gtactgtaga gtctggcgga tgtcgacaga ctctattttt
    12361 ttatgcagtt ttaactttgc agatagccgc attctcgccg gaattgcgtg attaatgacg
    12421 cggtcagtgg tgtctggcga ctatcacgcc gctgaatcaa ataataggcc gctttcggta
    12481 aaatttcgct aaccggcaac atcaccagcc cctgtccgtg caagggatcg cagcccattt
    12541 cttcaggcag tatgctgaga aaatcgcttt ttgccaccag actgatacag gctgagaacg
    12601 tctcgcagac tacaccgacc tgtggcgttt gcgcctgatc gtcaagcaat tcactcaact
    12661 gtttgtagta actgccgtgt ggcgtcggca ttgtccagct gtaatccagt aactgtttga
    12721 tcgaacgggc accaatggcg gggtgtcccg ggcggcaaaa gatcgcgaat tgcttttcca
    12781 gtaatttctc aaaagtaaat tcgtggtcgt acggtccctg ataataggta ttgatggtga
    12841 aatccaattc tccctgacgc aattcattaa tcatcgacac cagttgccct tccataatgc
    12901 gtacttttac ctgcggatgc tgctgatgaa aacgagatat gacagctggc atcagactgc
    12961 gggaaatact ggcccccatg ccgatattaa tctgccctgc cagttgccct tgtcgttggc
    13021 gaatttcctc ttgcgctgcg cgcagctctt caagaataag actggcgtgc tgataaaaac
    13081 tttcaccggc atcagttaac gtcacgcctt tactacggcg aaaaaagagt tgcgccgcta
    13141 atccttcttc cagctcctga atagatttac tcagtgccgg ttgcgacata ttcaacattc
    13201 ggctcgctcc gcgaatgctg ccctgacgag ccacttcaac aaaagcccga atttgatgaa
    13261 tttttacctg aaaagccatg acgccaccga taaccgttat ttatcagacc aaagaaactg
    13321 gcatctactt taatgcagat gattgtgtca gggtaattta tgaacggtta aaactgtgaa
    13381 aaatcagtta gtgataagta aaaactatcg ctacgtgaac cgggtcacac tttttactga
    13441 tgacgggaaa ggttatggag tctttgaatc aatttgttaa ttcgcttgcc ccaaaattat
    13501 cgcactggcg acgtgatttt catcactatg cagagtctgg ctgggtggaa ttccgcactg
    13561 ccacccttgt tgcggaagaa ttgcagcagc tcggctattc actggcgctg ggccgcgaag
    13621 tagttaatga aagtagccgg atgggactac ctgatgaact cactctacaa cgcgaattcg
    13681 agcgcgctcg tcaacagggt gcgctagcac aatggattgc ggtttttgaa ggtggtttca
    13741 cgggtatcgt cgccaccctg gataccggtc gccccggtcc ggttatggct ttccgtgtcg
    13801 atatggacgc gctggatctc agtgaagagc aggatgtcag ccatcgcccc taccgcgacg
    13861 gttttgcgtc atgtaacgcc ggaatgatgc atgcctgtgg tcatgatggg cataccgcca
    13921 ttgggcttgg gctggcgcat acccttaaac aattcgagtc cggactacat ggcgtcatca
    13981 aactgatttt tcagcctgca gaggaaggta cgcgtggcgc gcgggcgatg gtcgatgcag
    14041 gtgtcgtaga tgatgttgat tattttactg ccgtgcacat tggcactggc gtacctgcgg
    14101 gcaccgtggt gtgcggcagt gataatttta tggcaaccac caaatttgac gcgcacttca
    14161 ccgggaccgc cgctcacgca ggcgcaaaac cagaagacgg tcacaatgcc ttgttggcgg
    14221 cagcacaagc aactcttgca ctgcatgcaa tcgccccgca cagcgaagga gcttccagag
    14281 taaacgtggg cgttatgcag gcaggaagcg gccgtaacgt tgttcctgcc tcggcgttgc
    14341 tgaaagtgga aacacgcggg gccagcgacg tcattaatca atatgttttt gaccgtgcac
    14401 aacaagcgat tcagggcgca gcaaccatgt atggtgtcgg cgttgaaact cgcctgatgg
    14461 gtgcagctac cgccagttct ccttcgccgc aatgggtcgc atggttgcaa agtcaggcgg
    14521 ctcaggtcgc gggggtcaat caggccattg aacgtgttga agcgcctgcg ggttccgaag
    14581 atgccacatt aatgatggcc cgcgtgcagc accatcaagg gcaagcctcc tacgtggtgt
    14641 ttggcacaca gctggcggca ggtcatcaca acgaaaaatt cgattttgac gagcaggttc
    14701 tcgctattgc cgtcgaaacg ctggcgcgca ccgcgctcaa ttttccctgg acgcgaggta
    14761 tctgatgcag gaaatctatc gttttatcga cgatgcgatt gaagccgatc gccaacgtta
    14821 taccgatatt gccgatcaaa tctgggatca tccagaaaca cgttttgaag agttctggtc
    14881 agcggagcat ctggcttcgg cgctggaatc tgcaagcttc accgttaccc gcaacgtagg
    14941 caatatccca aatgcattta ttgcttcgtt tggtcaaggc aaaccggtta tcgccctgct
    15001 gggagaatat gacgccctgg caggtttaag tcagcaagct ggttgcgcgc aacctacatc
    15061 cgcgacgccc ggtgaaaatg gtcacggttg cggacacaat ttgctgggaa ccgccgcctt
    15121 tgccgctgca atagccgtca agaaatggct ggaacaatat gggcaaggcg gcacagtgcg
    15181 cttttatggt tgtcctggcg aagaaggcgg ctcgggtaaa acgttcatgg tccgcgaggg
    15241 ggtatttgat gatgtggatg cggcactcac ctggcacccg gaagcctttg ccggcatgtt
    15301 caatacccgc acgctggcaa acattcaggc atcatggtgc tttaaaggga tcgcagcaca
    15361 tgccgcgaat tcccctcatt tgggacgcag cgcccttgat gccgtaacgt tgatgaccac
    15421 tggcaccaac ttcctcaacg aacatattat tgaaaaagcg cgcgtacact atgccatcac
    15481 aaatagcggc gggatctcgc ccaacgtggt ccaggcgcag gcagaagtgc tttatcttat
    15541 ccgcgccccc gaaatgaccg acgtgcagca tatttatgat cgggtcgcca aaatcgccga
    15601 aggtgcggca ttgatgaccg aaaccacggt tgaatgccgc ttcgacaaag cctgttccag
    15661 ttatctcccg aatcgcacct tagaaaatgc catgtaccat gccctatccc attttggtac
    15721 cccggaatgg aactccgaag aactggcttt tgcgaaacaa attcaggcta cgctcacccc
    15781 caacgatcgg caaaacagtc tgaataatat cgccgcaacc ggtggcgaaa acggcaaggc
    15841 ttttgcacta cgtcatcgtg aaacggtact ggcgaatgag gtcgctccat atgccgccac
    15901 cgataacgtg cttgcggcat cgactgatgt cggcgacgtc agttggaaac tgcctgttgc
    15961 ccagtgtttc agcccctgtt ttgccgtcgg tacaccgcta catacgtggc aactggttag
    16021 ccaggggcga acatctattg ctcataaagg aatgctgctg gcggcgaaaa ctatggcagc
    16081 aaccacagtc aatctcttca ttgattcagg gctattgcaa gaatgccaac aagagcatca
    16141 gcaagttacg gacacgcaac cgtatcactg ccctatcccg aaaaacgtga caccgtcacc
    16201 tttaaaataa caacaacaac gcaaacacaa caaccgagga atgcccatga gtatgtcatc
    16261 cataccgtcg tcctcccaat ccgggaagct ctatggctgg gtcgaaagaa ttggtaacaa
    16321 ggttccccat ccttttttgc tctttatcta tttgataatc gtactcatgg tgacgacggc
    16381 aattttgtca gcctttggcg tcagtgcgaa aaacccgacc gatggtacgc cggtggtggt
    16441 gaaaaacctg ctcagtgtgg aaggattaca ctggttttta cccaatgtga ttaaaaactt
    16501 tagcggtttt gctccacttg gagcgatcct ggcgctggtt ttaggtgccg gcctagcgga
    16561 gcgcgtcggc ttactgccag cgctaatggt taaaatggca tcgcatgtta atgcccgcta
    16621 cgccagttat atggtgctgt ttattgcttt tttcagccac atttcttccg atgcggcgtt
    16681 agtgatcatg ccaccgatgg gtgcgctgat ttttctggcg gtgggcaggc atccagttgc
    16741 aggtttactg gctgccattg caggcgtagg ttgcggcttt acggctaatt tactgattgt
    16801 cacaaccgac gtgttgctgt cggggatcag cacggaagcg gcggctgcgt tcaatccgca
    16861 aatgcacgtc agtgtaattg ataactggta ttttatggcc agctccgtag tcgtactgac
    16921 gattgttggc ggcttgataa ccgacaaaat catcgagcca cggttaggtc aatggcaggg
    16981 aaacagcgat gagaaactgc agacattgac cgaaagtcag cgttttggtt tacgcatagc
    17041 aggtgtcgta tcgctacttt ttattgctgc gatcgcgctg atggtgatcc cggaaaacgg
    17101 gatattgcgc gatccgatta atcacaccgt gatgccatca ccctttatta aaggtatcgt
    17161 gccactaatc attctttttt tctttgtggt ttcgctggct tatggcatcg ctacccgcac
    17221 aattcgacgt caggcggatt taccgcattt aatgattgaa ccgatgaaag agatggcggg
    17281 atttatcgtg atggtttttc ccctcgccca gtttgtcgcc atgtttaact ggagcaacat
    17341 ggggaaattc atcgccgtgg ggctgaccga tatacttgaa agttcagggc ttagcggcat
    17401 cccggcgttt gtcggtctgg cgttgctttc ctctttctta tgcatgttta ttgccagcgg
    17461 ttccgcaatc tggtcgattc tggcccccat tttcgtacca atgtttatgc tacttggctt
    17521 tcacccggca tttgcgcaaa tcctctttcg tattgccgac tcatccgtat tgcctttagc
    17581 gccggtatct ccttttgttc cactgtttct tggattcctg caacgctaca aaccagacgc
    17641 gaaactgggt acttactatt cgttagtctt gccctatccg cttatctttt tggtggtatg
    17701 gctgctgatg ttgctggcgt ggtatcttgt tggtctgccg ataggtccgg ggatttaccc
    17761 acgtttgtct taagagagaa cggatgctga gattacttga agaaaaaatt gccacgccac
    17821 tgggtccact gtgggtgatg tgcgatgagc aatttcgcct gcgggcggtt gaatgggaag
    17881 agtacagcga acgcatggtg cagctgctgg acatccatta tcgtaaagaa ggctatgagc
    17941 gcatttctgc caccaaccca ggcggtttaa gcgacaagct tcgtgattat tttgccggta
    18001 atcttagcat tattgatacg cttcccactg ccacaggggg gacgccattt cagcgcgaag
    18061 tctggaaaac tctacgcact atcccctgcg ggcaggtaat gcattacggc caactggctg
    18121 aacaattggg ccgccctggc gcggcgcgtg ccgtaggtgc ggcaaatgga tcgaatccca
    18181 tcagcatcgt cgtaccttgc catcgggtta ttggtcgaaa cggcaccatg accggatatg
    18241 caggcggagt tcagcgaaaa gagtggttat tgcgccacga aggttatctt ttgctgtaaa
    18301 cattaaacaa tttgtgccag cttgttcaca cttttatgta aagttaccct taacaactta
    18361 agggttttca aatagataga catatattta catctaatat cggaattctc tgctgttaag
    18421 gtttgcttag acttacttgc tccctaaaaa gatgttaaaa ttgacaaata tcaattacgg
    18481 cttgagcaga cctatgatcc cggaaaagcg aattatacgg cgcattcagt ctggcggttg
    18541 tgctatccat tgccaggatt gcagcatcag ccagctttgc atcccgttca cactcaacga
    18601 acatgagctt gatcagcttg ataatatcat tgagcggaag aagcctattc agaaaggcca
    18661 gacgctgttt aaggctggtg atgaacttaa atcgctttat gccatccgct ccggtacgat
    18721 taaaagttat accatcactg agcaaggcga cgagcaaatc actggtttcc atttagctgg
    18781 cgacctggtg ggatttgacg ccatcggcag cggccatcac ccgagcttcg cgcaggcgct
    18841 ggaaacctcg atggtatgtg aaatcccgtt cgaaacgctg gacgatttgt ccggtaaaat
    18901 gccgaatctg cgtcagcaga tgatgcgtct gatgagcggt gaaatcaaag gcgatcagga
    18961 catgatcttg ctgttgtcga agaaaaatgc cgaggaacgt ctggctgcat tcatctacaa
    19021 cctgtcccgt cgttttgccc aacgcggctt ctcccctcgt gaattccgcc tgacgatgac
    19081 tcgtggcgat atcggtaact atctgggcct gacggtagaa accatcagcc gtctgctggg
    19141 tcgcttccag aaaagcggta tgctggcagt caaaggtaaa tacatcacca tcgaaaataa
    19201 cgatgcgctg gcccagcttg ctggtcatac gcgtaacgtt gcctgatttt tccgcataac
    19261 tcactatcct tctgtcatat cattaaattt ttctgattta ttgatctggc agaaggttca
    19321 tcactgtttc attcaccaga tatgggttaa tcttttaatt acaaactgcg ttgacagttg
    19381 ttgtaaggag accctgtatg gctatgtatc agaacatgct cgttgttatc gatcctaacc
    19441 aggacgacca accagcattg cggcgagctg tttatttaca tcaacggatt ggtggcaaaa
    19501 ttaaagcctt tttgccgatc tatgacttct catacgaaat gaccaccctg ctctccccgg
    19561 acgaacgtac cgctatgcgt cagggcgtca tcagccagcg tacagcctgg atccacgagc
    19621 aggcaaaata ttatctcaat gctggcgttc ccattgaaat taaagtggtc tggcataacc
    19681 gtcccttcga agccatcatt caggaagtga tcagcggcgg gcacgatttg gtgctaaaaa
    19741 tggcgcacca acatgaccgt ctggaagcgg tgatttttac gccaacggac tggcatctgt
    19801 tacgcaaatg cccaagcccg gtgtggatgg tgaaagacca gccgtggccg gaaggaggca
    19861 aggcgctggt ggcggtgaat ctcgccagtg aagagccgta tcataatgcg ctcaatgaaa
    19921 aactggtcaa agagacgatc gaactggcag aacaagtcaa ccataccgaa gttcatctgg
    19981 ttggcgctta tccggtaacg ccaattaata tcgcgattga actgccggaa tttgatccga
    20041 gcgtttataa cgatgccatt cgtgggcaac atttgctggc aatgaaagct ctgcggcaga
    20101 aattcggcat taatgaaaac atgacgcacg tagaaaaagg tctgccagaa gaggtgattc
    20161 ctgatttggc ggagcactta caggcgggga ttgtggttct gggcacggta ggacgcaccg
    20221 gtatttcagc agcattcctc ggcaacacgg cggaacaggt gattgatcat cttcgctgcg
    20281 atctgttggt cattaaacct gaccagtatc agacacccgt tgaactggat gacgaagaag
    20341 acgattaacg actcttcaat aactgacaag gcccgagtta tcgggcctat tttttactta
    20401 ctgctcttcg ccgccaagga aataaattcc caacgggatt gccagcaaca cagtaaagac
    20461 caggctataa acaaaaatca tcgatgactg aatcacatac attgatgtcg tccactcaga
    20521 cagcggaata ttgtactgtt ctataacgcc gccgatagtc gcgcgagtca caaccgacgc
    20581 cgcaataatg aaaaccgcca acaaaccaaa gagaaatttc ttccctttcg ctgacttcag
    20641 tttcgccata atcatgatgt aatccttgtg cagacaaatt tcttgttacc taaacgtaac
    20701 aataccatga catttcaccg ttgtctgctc tgaaaacctc acctcttagc tggatttaca
    20761 atcaacgcat tgattagtta gctaatgttg ctacttcctt ccctgactat tgatgatctg
    20821 aatgaattgt cgcggtaaaa tagccccgcc aggggtttcc ggcatgacga caattatctg
    20881 gaggagcctg ataaaacaat gatcgctgaa ctgtttacaa ataatgcgct taatctggtc
    20941 attattttcg gtagctgcgc agcattgatt ctgatgagct tttggtttcg ccgcggaaat
    21001 cgtaaaagaa aaggtttttt attccatgcg gtgcaatttt taatctacac cataattatc
    21061 agtgctgttg gtagcattat taattatgtt atagaaaact ataaactcaa atttatcacc
    21121 ccaggcgtta tcgattttat ctgtacgtcc ctgattgcgg ttattctgac gattaagcta
    21181 tttttgctga ttaatcagtt tgaaaaacag cagattaaaa aaggtcgcga tatcaccagt
    21241 gcgcggatta tgtcgcgcat tatcaaaatc accattattg tggtgcttgt tctgctttat
    21301 ggcgaacatt tcggcatgag cctttctggc ttgctgacct ttggtggtat tggtggtctg
    21361 gctgtcggta tggccggtaa agatattctg agtaatttct tttccgggat catgctctat
    21421 ttcgaccgtc ctttcagtat tggcgactgg atccgttcac cggacagaaa tatcgaaggt
    21481 acagtagcgg aaattggctg gcgaattacc aaaattacga cctttgataa tcgtccattg
    21541 tacgtaccga actcgctgtt ttcgtcgatc agcgtagaaa acccaggacg aatgaccaac
    21601 cgccgcatta ccacgaccat tggtttacgt tatgaggatg cggcaaaagt gggcgttatt
    21661 gtcgaagccg tacgtgagat gctgaaaaat cacccggcca tcgaccagcg acaaacctta
    21721 ctggtttatt tcaaccagtt tgccgactcg tcattgaata ttatggttta ttgctttacc
    21781 aaaaccacgg tatgggctga atggcttgct gcacagcaag acgtttattt gaagattatc
    21841 gatattgtgc agtcacacgg cgcggacttt gccttcccca gccagacgct gtatatggat
    21901 aacattacac cgccggaaca ggggcgctaa atcagacatc actggcgtta tccacaaggg
    21961 taacgccagt tcccatcaca tcaatgcttc acaatataca tagtccggct gtacgcaaca
    22021 tcttccgggt tattaatggg ataacctttc agccacggct tgattaatcg tccattggta
    22081 tattgataaa ttggtgcaat cggtgcttgc tccatgagga ttttttctgc cgcgttgtaa
    22141 tcggcattac gcgctttaac ggtattttcc atcgatgcct gggccagaac tttgtcgtat
    22201 gccggattgt taaagcgtga aatatttcct gaatgcgttg acgttaataa tgtcaggaaa
    22261 gtggagggtt cattataatc ccccacccat gaggcgcgga taacatcaaa attgccggtg
    22321 ttacggctat cgatataggt tttccattcc tgattttgca atttaacatc tacgccaagg
    22381 ttctttttcc acatcgatgc tacagcaatc gcaatttttt gatggttttc tgaagtgtta
    22441 tacaaaagcg tcagcttcag cggtttttgc ggaccataac cagctgcact caacaaagtt
    22501 tttgcctgcg cattcagttc ttcctgactc atttgttcaa acggcgaagg ttccggcgta
    22561 aatccggcgg taacatctgg tgtaaaatgc cacgctggct tttcgcccgt tcccaacact
    22621 ttttcggtca tcaggcggcg atctatcgtc atacttaatg ccaggcgaac gcgctgatct
    22681 gccgtcggcc ctttttgcgt gttaaacgca taataatagg tcccgagctg tggcggcgta
    22741 taaacctgcc ccggaatatc cttcaacagc ttctgataca tatttttcgg gaaggattcg
    22801 gtgatatcaa tatcccccgc gaggtaacgc ttagtggctg cggattcctg attaattggc
    22861 aggaaggtca ctttttgcag taccgttttg gcgttatccc aataatgggt attcggtacc
    22921 acgaccagtt tttcattgac tacgcgatct ttaagaacat aagcgccatt gccgatcaga
    22981 tttccgggtt tcgtccactc tttaccgctt tctacgttgg ctttttgcac cgggaagaag
    23041 gcaaagttag cggttaaatt cacaaaccac ggcaacggtt tatcaagctg aattttcaaa
    23101 gtatgggcat caactgcggt gacgccaagc tggtctggcg tagctttacc atcaataatc
    23161 gcctgtgcgt tgttgattcc cgccagcgcg gcaaaccatg caaatggcga tagcgttttc
    23221 gggtccacca gacgttgcca gctgtagaca aaatcttgcg ccgttaccgg tgtgccatcc
    23281 gcccattttg cgttatcgcg cagggtaaaa gtccagatac ggttgtcatt acttttccac
    23341 tgagtcgcaa cgccggggac aatctcccct ttctcgttct gattcaccag tccttcaaac
    23401 agatcgcgaa tgacctgaat ctctggcagg cccacggctt tagcgggatc cagcgacgca
    23461 ggctcatctt taatgtggcg caccagctcc tgcttctctg ccagtactgt gccgctcgga
    23521 acttctgcag catacgaaag agaaatgctg ctgaccaaca gcgcacaaca cgtgactgaa
    23581 acagagtgct tcataagttt ccctctaacc gacattaaaa ttgatgcgta attatttgtt
    23641 tcgccttaaa gaaatgcaaa caacttccat caaaaaagtg acacaggaag cataaaatgt
    23701 tttatgtatc aatatattaa atcagattta gtgtaaataa tcgacgccaa aatgcggtaa
    23761 agcacataga acaccgaaca ctacgttcag gcttcgcacg ttcaaaccag ttttcggtat
    23821 cataatcagc atctgtcgcc attgtacgaa gaataaaaaa taaccaaaac gttgaaagga
    23881 actgagaata cgttaagaga gaaattaaat taatgtatca gcaagtagtg ctaaacagca
    23941 cctttatact tcagcctatc tataaataat gagaatgccg agccagcatg tcgacggtgt
    24001 ggatagtaca ggtgatgacc gggtaaatct ggtgtgaatt tatccaaaac acgtatcagt
    24061 tttttttctt taatagcccg ctcaacctga tcataaggta gatacgccaa tccatgccca
    24121 tcaattgcag catcaatgat caggtctatc gtattcagta aaagctgacc ttccatgcga
    24181 acacgaactt cacgtccacc gcgtattaat ctccagcgat ttgctgtacc cgatgtggga
    24241 agatacaaat taattgcctg atgatctatt aattgtgaca ctgacgttgg aacacttcgg
    24301 cgagaaaaat aatccggtga gccaacaata gccattggta tatctggccc gattcgaatg
    24361 gcgatcatat ctttatccat ctccccaccc agacggacgc ctgcatcaaa acgttcagaa
    24421 acgacatcgg tcaaaccata atcaatggtg agctgaatat caatttcagg atgcgatttc
    24481 aggaatgtgc gcattgctgg taacaatatc gtttttgctg catgttctac agtagtaata
    24541 cgtattgtcc cggatgggcg gttctgcagc tcgctcaggg atgccagggc tgaatctatg
    24601 tcatgcaaca tagggccaag aacagacaaa agatgctcgc ccgcttcagt tggaacaacg
    24661 ctgcgcgtgg ttcgcgtcag aagccgcaat cccaatcgtt cttctatacg acgcactatc
    24721 tggcttaaag ctgactgcgc catgctcagg cgggctgctg cacgagtgaa gctacgctcc
    24781 tctgcaacga cgacaaacgc catcagatca gcgatttctt cacgcttcat tcagtatctc
    24841 catgcaatct attaatcatt taaatgatag gtctaatcat gattttgggt ctaatcaact
    24901 taatgatcaa tatctatagt gatatcacca accgttaaaa cgaaccgaca actccatgag
    24961 gaacggaatg atgaataata aagtcagctt cactaacagc aataatccaa ccatctcttt
    25021 gtctgcagtg agctatttcc ctccaaaatt tgatgaaacc cgtcagtatc aggcaattgt
    25081 ggtatctcac ccaggtgggg gcgttaaaga acaaacggcc ggaacctatg ccaaaaaact
    25141 ggcggaaaag ggatttgtca ctattgctta tgacgcatct tatcaaggtg aaagtggcgg
    25201 cgagccgcgt cagttagaaa acccatacat tcgtactgaa gacatcagtg ccgtgattga
    25261 ttacctgaca acgctttctt atgtcgacaa tacccgaatt ggtgcgatgg gaatttgtgc
    25321 cggtgcagga tatacagcca atgctgcaat tcaggatcgc cgcatcaagg ccatcggtac
    25381 cgttagtgcg gtcaatattg gttcgatatt ccgtaatggt tgggaaaaca atgtgaaatc
    25441 tattgatgcc ctgccatacg ttgaagcggg ttcaaacgcc agaaccagcg atattagcag
    25501 cggagaatac gccattatgc cactggcacc aatgaaggag tccgatgcgc cgaacgaaga
    25561 actgcgtcag gcctgggagt attaccacac ccctcgcgcg cagtatccaa cagcaccggg
    25621 ttacgctact ttgcgcagcc ttaaccagat tattacctat gatgcttacc atatggcgga
    25681 agtgtacctg actcagccga cgcagattgt ggcgggcagc caggcaggga gcaaatggat
    25741 gagtgacgac ctgtacgatc gagcatcaag ccaggataaa cgctatcaca ttgttgaagg
    25801 cgcaaaccat atggatcttt atgacggcaa agcctatgtg gctgaagcaa tttcagtatt
    25861 agctccgttc tttgaggaaa ctctgtaatg aaacatattc aaatccgcaa tagtgatatg
    25921 gcctggcata ttgctgccaa tattcagttt ccaccaaatt ttgatgagtc aaaacagtat
    25981 ccggcgatta tcagcgtcca tccttttggc agttgtaagg agcagacgtc tggcaatatc
    26041 tatggcaaag cattggctga aaaaggctat ctcgttctcg cttatgacgc cagtttccag
    26101 ggagaatctg gtggggagcc tcgctggata gaagatccga cacagcgcgt agaggatatt
    26161 agccgtgtta tcgattatgc agtaactctg ccatatgtta atgctgaacg tattggcgtg
    26221 ctgggagtgt gtgggggtgg tggctatgcc attaatgccg cgctgacaga aaaacgaatt
    26281 aaagcagtag tgagtattac gggtgttaat atcgggcgtc tttttcggga aggtttcagc
    26341 aattacgatc cgattggcgc attgaatgca atggcgtcgc aacgaaccaa agaggctcgt
    26401 ggtggagaat tacagatcaa cgaattactt cccgccagcc tggatgcagc aaaagcccat
    26461 ggactgaccg aacgcgatgt ttatgaagca actgactact ataaaacacc acgcgggcaa
    26521 caaccgggag gtgcgacaaa aatgttgttc tcacatgcac agaaaacgct ggcctgggat
    26581 gccttcgcat ttactgaagt gttactgact cagccggtga tggtagtagt tggtgaaaaa
    26641 gtcggtgcct tcggtgctta tcgtgatgga ctggaagtct atggccgggc gatggtttcg
    26701 caggatcgtc agttagtttc attacccgat ttctcacatt atgaactgta tgacaaaccg
    26761 gaagcagttc agaaggcgct ggaaaaagtt attccttttt tcaacaccca tctgggataa
    26821 gtttcatgag aaatattatg aaaaatgtac tgattcttgg tgctggcggt cagatagccc
    26881 gccatgtgat taatcaactg gcagataaac aaaccattaa acaaacttta tttgcccgtc
    26941 agccagcaaa aatccacaaa ccctatccaa ctaatagcaa gattattatg ggagatgtac
    27001 tgaatcatgc agcactgaag caggccatgc agggacagga cgttgtgtat gccaacctta
    27061 cgggggaaga tttagatatt caggcaaatt cagtgatcgc tgcgatgaaa gcctgtgatg
    27121 ttaaacgtct gatctttgtt ctttccctcg gtatttatga tgaagttcca ggaaaatttg
    27181 gcgaatggaa taatgcagtt attggtgagc ccctaaaacc attccgcagg gctgcggatg
    27241 caattgaggc ttctggactg gaatatacaa ttcttcgccc tgcctggctg actgatgaag
    27301 atattattga ttatgagctt acctcacgca atgaaccatt caaaggtacg atcgtatcac
    27361 gtaagagtgt cgcagcatta atcacagata tcatcgataa acctgagaaa cacattggtg
    27421 agaatatagg tatcaatcaa cccgtgactg atggtgacaa gccattattc atgtaaatca
    27481 attacatagt taccatcgta atgtactatg tgcagtacga tttattaaac ttattaaggt
    27541 gaactgatca atccgatgcg attaacatgt gtagaaccag taagaataaa atacgccatg
    27601 tatataacat tacgacttac atctaagtca gcataattct aatatctgca catcaataaa
    27661 aatgcaaaca acttccggca aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa
    27721 ggatttgata atccgcaaaa tttgcacaac actgcctgta atacttcctg aaccagagac
    27781 gatcatgacc gtaaaccgcc cacgcgccga acgcggcgca tttccgcccg gaacagaaca
    27841 ttacggacgt tcattattgg gcgcgccgtt aatctggttt ccggccactg cagccagtcg
    27901 cgaaagtggt ttgattctgg ctggcactca cggtgatgaa aactcttcgg tcgtcacgct
    27961 ctcctgtgct ctgcggactt tgacaccttc tttgcgccgc catcatgtag tgctgtgtgt
    28021 gaatcctgac ggctgccagc tggggttacg ggccaatgct aatggtgtgg atttaaaccg
    28081 aaactttccg gcggcgaact ggaaggaagg tgaaacagtt tatcgctgga acagcgccgc
    28141 tgaagaacgt gatgtcgtat tgctgacagg tgacaaacct ggctctgaac ctgaaaccca
    28201 ggcgctgtgc cagcttatac accgcattca acccgcctgg gtggtctcat tccacgatcc
    28261 gctggcctgt attgaagatc ccagacacag cgaattaggc gaatggctgg cccaggcgtt
    28321 tgaattgcct ctggtaacca gcgtcggtta tgaaacacct ggctctttcg gtagttggtg
    28381 tgcggatctc aacctgcatt gcatcaccgc agagtttccg cccatctcct ccgacgaagc
    28441 cagcgaaaaa tatctctttg cgatggcaaa cttgctgcgc tggcatccta aagatgcaat
    28501 tcgcccgtcg tgaactgaag cgccggttcc acatctaccg ccagccaggt cggtccgtca
    28561 agatcgacga aactgacctg cggcaccagc ggtaaagcgg cgctaatggc ccgagaggta
    28621 cacaacatgc agcccagcat cagacgaaaa ccttgtgcac gcgcttcagt cgccagcgcc
    28681 agcgcttccg tcagaccacc ggttttatca agcttaatgt taaccatctc atagcgccct
    28741 ttcagcgcct tcagattgct acgtgtatga caactttcat ctgcacaaat cggtaacgga
    28801 tgaataaaat tctccagcgc cgcatcgtcc tgcgcaggaa gcggttgttc aagcatcgca
    28861 acgcctaaat ccgccaatag ctggcaacgc gccgccaacc cttctgcacg ccaggattca
    28921 tttgcgtcaa cgatcagcgt cgcatcgggc acagctgtgc gaattgccac catccgctca
    28981 ctgataagat ggttatccag cttcactttc agtaatttcg cgcctgcctg ccagagtgtt
    29041 gatgcactat tggccatctg atcaggcgta ccgatgacta ccgtctgtgc agtggtgact
    29101 gtcccgggaa gcgttatgcc gatcaaatca gccagcgatt gctgctgttt tcgcgcggcc
    29161 agatcccaca atgcacaatc cagcgcatta cgtgctgcgc cggcagggag aattttttgc
    29221 aactcctccc gtgtcagccc tttctctaat tgcggcacga cgctcataat ttgcgccatt
    29281 accgaggcat cactttcccc ataacgcgga tacggcgtgc attcgccggt gcctttaata
    29341 ccctcttctt ccagttcaac caccaccacg cgcgcttcac tgcgacttcc ccgggcaatc
    29401 acaaacgggg tatgtaaggg ccaggcttcc tcaaatacct taacggttct catctttact
    29461 ccttgttgtc gcggtggtct gcggattaag cgcaaatagc gtttgctgtg ttattgacag
    29521 ttagcataaa ctaggtgtga cgttaactat atgtaaacag gaaagataat catgtcacaa
    29581 accgttcatt tccagggcaa cccggttaca gtcgccaatt ccatcccgca ggcgggtagc
    29641 aaagcgcaga cttttactct cgtggcaaaa gatctgtctg acgtcaccct cggtcagttt
    29701 gcgggtaaac gcaaagtgct gaacattttc ccgagtattg ataccggtgt ttgcgccgca
    29761 tcagtacgta agtttaacca actggcaact gagatcgaca acaccgttgt gctgtgtatc
    29821 tctgccgatc tgccgttcgc ccagtctcgt ttctgcggcg cagaaggtct gaacaacgtt
    29881 atcaccctct ccactttccg taacgctgaa tttctgcaag ccttcggtgt ggcaattgct
    29941 gatggcccac tgaaaggtct ggcagcgcgt gccgttgtgg ttattgacga aaatgacaat
    30001 gtgattttca gccagctggt ggatgaaatc accaccgagc cggattacga agcagctctg
    30061 gctgtactga aagcataagt taatatgcct gatggtgttg caccatcagg catattcgcg
    30121 cttactcttc gttcttcttc tgactcagac catattcccg caacttattg gcaatcgcgg
    30181 tatgtgaaac gccgagacgt tttgccagtt tgcgcgtgct gggataattg cgataaagct
    30241 gggttaatac cgagcgttca aaacggctgg tgatttcgtc cagcgaacct tccatcgcat
    30301 cttcgcccac ggctaccgtt gcggcgtcat aatccggcaa caaaatatcc tgtggacgca
    30361 gctcataacc gtccagttgt gtcagtgcgc gatagatagc gttctttaac tgccgcacat
    30421 ttcccggcca cgcataacgc gtaagtacag tattcaggtc agcggccagt ttcggacgcg
    30481 gcacgccctg ctcgtcggca aagcgggcga cgaacagctc agttaacggc atgatgtcct
    30541 gcggacagtc acgtagcggc ggcagattga gcgtcaacac gttcagacga taatagagat
    30601 cttcacggaa catgcctttt tgcaccagtt cgaccagatt cttctgcgta gcgcaaatca
    30661 cccgcacatc gacatgcacc tcatggtctt cgccaacccg acggaaagtg ccatcattaa
    30721 ggaaacgcag taatttcgcc tgcatccgtg gtgacatttc ccctatttca tccaacagca
    30781 ccgaaccacc gttcgcctgc tcaaagaatc ctttcttccc ttccggagca tgaccaaaca
    30841 gttcactctc gaccgcatct tccggtatag acgcacagtt cagcgccagg taaggtttgc
    30901 ccgctctggg gcttgcctga tggcaggcgt aggcaaagag atctttacct gtacctgtgt
    30961 cacccgtaat cagcagcggc gcgcttagca tcgccagttt ctgcgcctgt tcgacaacat
    31021 gcttcatttt cgggctgacg gcgacaattt gactgaaggc gctgacgtcc tgggcggcga
    31081 cattttgcaa ctggcggccc atacgaatcg ttgatcgcaa catcaccacc gcaccggtca
    31141 ggacgtgttg atcattttca tcctgaagat aaacaggcgt aatctccatc aggaaattct
    31201 gcccattaat aacgacatgc tcgttatggg aatcttgcgg ttcgctttcc agccaacgta
    31261 aaaaattaaa gccgttaatc aattgtgcgg cggtatggtt gcgcaggcga tccaattttt
    31321 gcgcaaaaag ctgacagctc gccgggttcg ccatatccac tttgcttttc atatcgacag
    31381 agagcacagg ttcgggcaac gcctccagta acgcgctcaa cgccagatgc tcacgttcgg
    31441 aaggcatcca cgggacagta cgcacatcgg taacacctgc aatacggcgt atttcggcca
    31501 tcagactgct gaaactctca aactccagtt cagcaaaatt gaggtagatt cgcccaatgg
    31561 gatcaatctc aataccgcgt aaatcaatgc ctcttagcac gagtagatcg agtaattcgc
    31621 gggtcagacc gagtcggtct tcacaaaaga cttccagacg catgggaacc ttcacctgaa
    31681 aaaagaacaa ttaatatgat gatatgacac tatttggata gcaggaaggt ttctgtcaac
    31741 aatcattgac acaaagacgg aaaaacgggc acttcggcgt aaagatatcc ggtcagcacc
    31801 cgatattgca ttatttttcg ctgggcgttt tgcctttttg cagcgtttct ttcacctgac
    31861 cgataagctg acgacggaaa tccccgaggc gaggtttgcc atcgtcaatc cacggcagcg
    31921 ggcggcaaag ctccatagct ttaatcccga gtcgtgccgt cagaagtcca gcaccaattc
    31981 cctgagctgc gcgggtagac aaacgagcag cgagatcttg cgacatccag tccatcccca
    32041 cttcgcgcac cagttcgctg gctccggcaa aagcgatatt cagcaatacc agcttaaaca
    32101 gacgcaaacg gctgtaatac cccagttcaa tgccatacag cgtggcgatg cgattaatta
    32161 aacgcagatt gcgccaggcg ataaacgcca tatcgaccaa cgccagcggg ctgaccgcaa
    32221 tcatcaacgt tgattccgcc gccgaacggc tgatttcgcg ccgcgcctgg gcatctaaaa
    32281 ctggctggac caaatgcgca tacaaactga ccacttcacg gtcgttttgc gtttcatgga
    32341 ttgaggcata ccagcgttgc agcgctggat gagactgatc aatacccgcc tgctgcgcca
    32401 gtttttcgca aaatgcgcgg cctttgcccg tgccgtggct gtgcaacaaa tcgcgcgctt
    32461 cgtcgcgttc atgggcgcgc tgtcgcaagc gccataagcg ccgccactct gttaccacag
    32521 aacctacgcc agcgccgata atcaatgccc cagcggcaca tccacccagc gccacccagt
    32581 cctgagtttg ccaggcattc attgtccact gtacaccctg cccgacaacg cttgcgccaa
    32641 acagagccag cccgcccatc accattttgc gccacaggct gcgtttcgga cgtaacgctg
    32701 catccattac cgcttcaact tgcccctctt cttcaggcgc ttcatcgagc gtggccgggg
    32761 caaaattttg cgcctgattt tcgtcaaagg tctgctgcgc cctgaatttt ggattctgat
    32821 cgacctccag cggaccgtcg aaatcaatac gtggttttaa cggttcggtc atcgcaattt
    32881 atctcctatt aaaaattcaa gcgcggcatc aagacgaata tgcggcagcg gtttgtcgac
    32941 atccatcacc tgcgggcgaa acgcttcaaa ctggaaccct tgcttgtccc agaacgcctg
    33001 accgggcaaa cgcgcaggaa cttcaccggg ataaacggtg agtggcgcac catcgctgag
    33061 ccggttaccg cgcaatgccg gaattttctc gccgttgacg tcaataatgc cgctggtggt
    33121 cgcctgcacc gaggccagcc ccaggcaatc catactaatc ccttcaaacg ccgcattttg
    33181 ccaggcatcc tgaatcagtt gttgcagtaa tgaaaccata ttggcgtgct gatcgatggt
    33241 cacatggtcc gctttagtgg cagcaaacaa tagcttatcg ataaccggcg aaaacaaacg
    33301 ccggaacaag gtacgctgcc cgtagtggaa actttgcatc agctgcgtca gtgccagacg
    33361 catatcatta aatgcctgtg gcccactgtt gagaggttgc aggcaatcaa ccagcacaat
    33421 ctggcggtca aagcgcagaa aatgattctt atagaacccc ttcaccacct tctcgcagta
    33481 ataattaaac cgctcgcgca gcattccggc attggtgtgc ttatcggcct gcgccagttt
    33541 ggactcgccc caggcatcga catccggcca cgggaagaat tgcagcgcgg gcgcacctgc
    33601 catatctccc ggcaagacaa agcgcccagg ctgaataaag tgcagcccct gctgtttaca
    33661 gtggtggaga taatcggtcc acgcggcggc aatgtctgcc agccggtttt cgtcggcagg
    33721 cgctagcggg tccagccctt cgcacatcat tcgccatttg accgaccatt cgccgcgctg
    33781 accattgagt aagcccgtca tctggcgtga ccagcttaaa tagtcctgcg ccagcatcgg
    33841 caggtcgagc aaccattcgc cgggataatc cacaatctcc agatacagcg tggaggtgtc
    33901 cttgaagtgg cgtagcagcg aatcgttcga tttaaaacgt agcgccaggc ggatttcact
    33961 gacgccgcgc gttggcgtcg gccaggcggg aggatcgcca tataactgcg ccagtccttc
    34021 atcatatgtg aagcgcggaa tgccaaagtc acgctgagga atgcgtttta cgcccagcag
    34081 gcgctcttca cgcaccgcgc ttaacagcgg caagcgtgct ccggcgtgaa tattgagcaa
    34141 ctgattgacc atcgcagtga taaacgctgt tttgccgctg cggcttagtc cggttacggc
    34201 gaggcgcaga tgtctgtcga caccccgatt caccagcgca ttaagttcat ttttaagtcg
    34261 cttcattgct gtcctgtgct gctctgaaga ccagaaaaaa atcattcgta aggatatgaa
    34321 tgtgggggcc aggccattga ttatcaactg cccgtgttgt catcgacggg cgaactccat
    34381 aagatgtgca gcattcacgt tcggacggag ttcctgacct atgtccccta ctatttatga
    34441 tattgccagg gttgcaggcg tatcaaaatc caccgtatca cgcgtgctga ataagcaaac
    34501 taatatctcc ccggaagcgc gcgaaaaagt gttacgggcc attgaagaat tacagtatca
    34561 gccaaacaag ctggcccgcg cgctgacctc ttcgggtttt gatgccatta tggtgatttc
    34621 tacccgttcg accaaaacta cggcgggtaa tccgtttttc tctgaagttt tgcatgccat
    34681 caccgccaaa gctgaagagg aaggtttcga cgtgatattg cagacgtcgc acaatccggc
    34741 agaagactta caaaaatgtg aaagcaaaat taagcagaaa atgattaaag gcattattat
    34801 gctaagttcg ccagcggatg agtcattttt tgcccaactt gataaatacg atattcctgt
    34861 cgtggtgatt ggcaaagttg aaggtcaata tgcccatgtt tattctgtcg ataccgataa
    34921 ttttggcgac agcattgcgt tgaccgatgc attaattgaa agtgggcatc aaaatattgc
    34981 ctgcctgcat gctccgcttg atgtccatgt ttcagtggat cgggtaaatg gttataagca
    35041 gagcctggct gcgcataata ttgcagtgcg tgatgaatgg attgttgacg gcggttatac
    35101 ccatgaaaca gcactgcaag ccgcgcggga attattaagc cagtcaccgt tgcctgaggc
    35161 agtgtttgct actgacagcc tgaaattaat gagtatttat cgtgcggcag cagagaaaaa
    35221 tattgctatt ccgcagcagt tagcggtggt gggttatagt aatgaaacgc tgtcgtttat
    35281 tttaacgcct gcgccaggcg gtattgatat cccaacacag gagttaggtc aacaaagctg
    35341 tgagttatta ttccgcttaa tttctggaaa accgtcacca cagaatatta ccgttgccac
    35401 gcatatgacg ctgaaataaa taccagctac cttgcctgaa tgccggatgc gatgctagcg
    35461 catcttatcc ggcctacatt tatagttcgg ataaagtact cgtgccgcat ccggcaattt
    35521 agcccattat cagaacgagt aatttacgcc gacacctgca taatggaatt tatcgctgtc
    35581 gccttcgtcg tgatcctgcc actcaaacgc gtattccagc gaaacggaaa gcccgttctg
    35641 gaaatcataa ccgtaaaata aacctacacg gttaaaatca tggccttcac gttcaatatc
    35701 atcctgccag tcccagttac tccagcgatc cagcccaatg cgcgtatacg gcgtcaccga
    35761 gtggttgccg agcgtcagcg gcaaataggc gcgaatttct tgcgtagaaa actcaccgtt
    35821 attgcggctg tcgtccatat taaagccgcg ctcgagataa tagttcactc gcaaggcaac
    35881 cgtttcgttg aaggtatatt gcaggcctgt ttccgtttcg actcgggtat cagcgtaacc
    35941 ggtcgtgttc agatcgttgg caaatttata catcgacaac caaccgttga aacgtaaatc
    36001 gtcagtcagt ttcacatccc agtctggcgc gattttccag cgctgcatat tcgccgtgtc
    36061 tttacccggt tcatcaacgt agtgataacc ataattacgg aaaccgccgg tcaggccgaa
    36121 actgaaatca tcgttttcga ggaactgata atgcacctcc agctccgggc gatcaaacca
    36181 cgttccacgt ttacccgcgc tataatctac cggcccttcc tgataatagg ccagagcaat
    36241 tctccacggc ccgttggcgg cattaaaata gactgaaggc tccgccagcc catccatatc
    36301 ttcgccataa ccctcgacgt tttctatttc gtacatcgcg ccgatattaa agtgccagtc
    36361 gttcctttcc tcggcctgtg cgcaggccat tcccgcacac atcaccagtg cggtacaggg
    36421 taataacttt ttcattattg ttccttgtat tattctgggg gaatgccttc cccctgtatt
    36481 tagcgaattg ctatttccgt ttctgcatca aagaaatgac atttcgtcat atcaaaatga
    36541 atagtgatat tttctcctgc atgataatca tttaacgcac cagcacggac cactaactcg
    36601 tgccccccaa ccgtggtgta gagcataaat tccgcaccgg ttaattctgc cacgctaatt
    36661 ttggcggaaa tgttattttc ctcttgcgcg tccggatgaa tatcttccgg acgtattccc
    36721 atcacgatgg gcttatgcaa acttccctgt gttttcagaa ccgctaattt ctcttcggga
    36781 atggttaatt taagcgtttc cgtaacgaat ttatcgccat cgatcgtgcc acgaataaaa
    36841 ttcatcgccg gtgatccaat aaatccggca acaaacatat tcgctggttg gttataaacc
    36901 gttttcggcg caccaacttg ctgaacaatc ccatccttca taatcacaat ccgcgtcgcc
    36961 atggtcatcg cttcggtctg atcgtgggtc acgtagatca tggtggtatt cagcttctgg
    37021 tgtagcttgc tgatctctgc gcgcatttgc acgcgcagct tggcatcaag gttagagagc
    37081 ggttcatcca ttaaaaacac gcccgcttcg cgcacaatcg cccgcccgag cgccacacgc
    37141 tggcgttgcc cgccggaaag cgcgcccggc ttgcgtttca ggtactcacg caggccgaga
    37201 atttgcgccg cccagttcac ccgctcatca atcacctctt tggcgatttt ttgcatcttc
    37261 aggccaaacg ccatgttgtc gtaaaccgtc atatgcggat acaacgcgta gttctggaac
    37321 accatcgcta tattgcgtgc tttggctgga acgtcattca ttcgtttgcc gtcgatcaac
    37381 agatcgccgc cgctgatctc ctcaagcccg gcaatcatgc gcagggtggt cgacttaccg
    37441 cagcccgacg ggccgacaaa cacgatgaac tctttatcgg caatttccag gttgaagtcc
    37501 ttcaccacat gcacctggtt atcgtagatt ttttgaatat gttgtaacga aagctgagcc
    37561 atgttgattc ctttgctata cgttttgcca gaaggccgat aaccgcgacc aggtcagaga
    37621 gtccgttgaa ggcaacagta attgcgcccc ggttaagccc gcgccgatcc ccaccgagcg
    37681 cataccgctg gcgttaatgg cgtcaatgcc cgcctgcgca tcttcaatgc cgatacatgc
    37741 ctgcggcggc acgcccagcc cggcacaggc ggcgagaaag atttccgggt ccggtttcga
    37801 gtttttaagt tgggaagcat ccgcgcagaa ggtgaaaaac tcgcgcagct ccagcgccgc
    37861 taaaatcgtc ggcgcattca gggagacaga agccagccca accgggatct gctgtgcacg
    37921 gagatctgcc agcaaagagc gaatgccggg tagaacagcg ctgaccgtca actcgcgcag
    37981 tgagtggaca tagagcagat ttttgcgata cgccagttgc gccctctcct gcgagttaaa
    38041 gtcgccctct ttgcccccgt gttgcagaat gcgccgcaga gactcatcgc ggctgatccc
    38101 ttttagggat tcgttaaact gcgcatcaat gctgatgcca atttcagcgg caatctgctg
    38161 ccacgcctgg aaatgcagat gcgcggtatc ggtgattaca ccatccagat cgaaaattac
    38221 cccttgcagt ttcattcatc ctcctgatgt gtggtagcgg tcccattgaa gggcaaaata
    38281 aaatcaccca aacagaaaac agattcttca gccacggata taagctgacc gttcaaacgc
    38341 agtgaaacgg gcgcagaagt tcgaatcgca atacgctgcg cgtcaagagt gacctgtaat
    38401 tcgcagccct gccagaacag agggaaagac aactgttgcc actgctcagg taacgccgga
    38461 ttgagatgca attcaccgtc acgcacgctc accccggcaa aaccctgaat cgcccccagc
    38521 cagatagcgc cagttgcggc agcatggata ccatcatcac aactatgcgg atcagcacca
    38581 agatcgattt cagtcccctc gcgccagaac tgataacttt gggtcagcag gccacagcgt
    38641 gcggcaacaa tgccgtggat tgctttactt aatgacgagt cgtgaatagt gcgcggttca
    38701 taaaattgca gattggcaag acacgatgcc gctgagaact gctccggcag catgtaattg
    38761 agcatcacca catcagcttg tttgaggatt tgcatctcgt tcacttctgc gcgtgaataa
    38821 tccagcagaa tggtttgctt ccccgccgcc gctttgtatt tcgccagatt aatcgccggc
    38881 ttagccataa acgaatcatc ctgcggcaaa acgccgtcgg gctgaatttc tggcagccgc
    38941 agttctttaa ggaacatttc ggcgcgatgg ataaacgcat cgtcgctaca gccaaactgg
    39001 cgggcaatac tcagcgcctg ctggacgttg taatacgcca tatagctggt gaaggcgtta
    39061 ttattgacat gttcggtata ttcgtcaggc ccaataacat catgaatttc cagacggtcg
    39121 ttaaccctca ccgcgcggct aatccagaac tttgcagttt ccagaagtag cgccatgcct
    39181 tcatgcgcaa tgaaactttc atcccccgtg gtctgccagt attgaataac cgcccaggcg
    39241 atatcggcca ccagatgatg ttccgcctgc gccgaggcca ctttttgccg cagcccggtg
    39301 cgaatgttaa tggcggcaaa ttccggcgtc tcttcttcgc cgctgcgcgc gctttcccac
    39361 ggaaatagcg cgccctgcca gccgttgcgt cgcgctttct cctgcgcgcc tggcaagttg
    39421 tgccagcgat aacgcagtaa acttcgggca accgtcggat cgctaaacag atgaaacggt
    39481 aacaaaaata cttctgtatc ccagaaaacg tggcctttgt agccttcgcc ggttaagcct
    39541 tttgccgcaa tgctgctgcg ctcgtcgtgg gcaggcgtca tgatgcgcag atgataaagc
    39601 gcataatcta acgcttgctg atcgtgcgct tcgccgccat ttaccgtgat acgacgtttc
    39661 tgccaccatt gacgccagtt ttctgttgat actgcaagaa gttggtcgta actctgctgc
    39721 gcgcacattt caagctggcg aagcgacgcg ctgccccact cgtctaaaac ggcttgcctg
    39781 tcatcccgcc agtcgatcca caccagtttt tgcaacgtca ctgtctcgcc tgcatgaagc
    39841 tgcgcactgg tatgttgctg caaacggcgc tctttggcgg tatagcattg ctgcacatca
    39901 ccgctcacca tacagcaaca gctgatggcc acatcactgc gtccatcctg ggtggtgtag
    39961 atcccctgca tcagatgctg accaaacacc cgcacctggg tttcgtcgag atgttggcga
    40021 ccgtggttgg tttgcgtggc gtcgatacct gttgaaatca gcactgacgc gtcggcgtcc
    40081 agtggcgtaa tagtgatttc cagcacaatg agcggcagtt ggtctgccga aacaaagcga
    40141 cggctggcga tggtgtaacc tgtgccgttg ctggtacgcc agacaacgct acggcgtaat
    40201 tctccactgg caaagtcaag ttcacgctgc caggcttcgc gggataacga gaaaacctca
    40261 ccatttatgg caatctccat ccccaagata tcaggcaggt tcaccagttc gttgatttca
    40321 ccttttcccg cccgatgata cagccccgcc agatacatcc ctcgcgtctg gcgcgtgtaa
    40381 tcttcttcat ggctggcgcg aagcccaaga tagccgttcc cctgcgccat cagcgatgca
    40441 tacttgttca gggtatgctg gctgaaatgg ggttctgata acgttactgg cctggtcata
    40501 attccacacg cgtccctgtt tctgcggatt gatacaacgc cgccaccagt tgctggatga
    40561 tgtacccctg ctcggcgtcg gctatcatca cgggcttgcc ctgtacgtga ttgataaagg
    40621 cttccatgct gcgcaaatgg cggttgtcgt ctgccagttc ccgttgcatc agcgtcatta
    40681 attcaccgtt gttatcggtg tagatatgtg ctggaaacag cgtcgcaccg gctttatcac
    40741 cacaaaagct gacgttcata atcgactgtt cgcggatgtt gagtgcaaat gacgtttcca
    40801 gccacagaat gccgccgtta tgaaattcaa tggtgccaaa taacgaatct tcgacgctgt
    40861 aagttgccgg atcccactcg ccaaattgac cgcagctctt ttgcgtgccg atcttttgaa
    40921 agctatgcgc attcacgctt ttcaccgccg gaaagcccag cacatacatc gccgcatcca
    40981 gcatatgaat gccgatgtcg ataagcgggc caccaccctg cagttcttta ttggtaaaga
    41041 caccccagcc gggaacgccg cagcgacgca gggcgcgggc ggtggtaacg taaatctctc
    41101 ccaaaacgcc gttggtcacc tgttcacgca gctgttgcgt atcgagcgca aaacggtggt
    41161 gaaagtcgta ggccagcacc ttgcccagtt tgcgcgcggt atcgcacatt tcccgcgcct
    41221 gttctggcgt catggcgggc ggtttttcgc acatcacatg gcagcccgct tccagtgcca
    41281 tcagggtatg ttcgtaatga aaacggttag gtgagcagac gctaaccaca tcaggtttca
    41341 ccgccagcag catggcctgc ggatcgtccc acacggatgc attaccgtat ttatctgcca
    41401 gcgcctgcgc ctgggaaagg cggctgtcac agacagccac cagttccaga tcgttgcggg
    41461 tgcagtagta cgaagcatga accttatccg ccacctggcc tgcgcctatt atcgcgaccc
    41521 gcagcggaga gcttgtcatt gcacttttca caaagacctc ttagcaggta cgcaaccagg
    41581 ccaacgaatc acggtacgcc tgggcaggct cttccgcccg gatacgccct tcgtacacta
    41641 catagccctg atagttatcc gcgcgcagct gttcaaacag cgcgtggaaa tccagggtgc
    41701 cgctgcccgg ctggtagcga tggttatccg caatatgcac atgaccgagc aggtcgcggt
    41761 tgtcatgcag cgcctgcgcc aggttatctt cttcgatatt catgtgatag aaatcgccga
    41821 taatctgtac atgtttaaga tcgttttcga cgatgtaacg gcgggcatcg gcgagggtgt
    41881 tgatcatatg atcctgatag cggtttaacg gttcgagata caccacggtt ccggtacgcg
    41941 cggcgacctg ttccagtacg tgcagggaat cactcaccat tttgcggtcg ccgtccaggc
    42001 tacgcggcga ggtcatcggc ggtaagcgga aggtaaacat gccccacgct gccggaacga
    42061 cgataccttt accccccact tctgccagcg cttcgagaat gcgttcgatc tgctttaagc
    42121 cattaagacg acgctcttcg ataaagtcgc caatccaacc gtcatagcca ccgcaggcgg
    42181 tggtcaccgg taaaccggtt tctttgattg ctgctttgac ttcttcgaga ttgttgacca
    42241 gcagtttgcc gtcaatctca aaaccatcga agcccatctc tttgatataa cgaaatttct
    42301 ccagaatgtt ttccggaaag aatgcctgat tctgtgtgcc tattttcatt ttttcattcc
    42361 tttgaattaa aacgtaacgc ccattttgat gctctgttcc ggatgctgat cgacatactg
    42421 catgtagctt tccgggctct tggcaaaggt cactaccggg tcgattaaat cttcgcaatt
    42481 gagataaccg ttcatcagca gttcccagca ggtttcttca atacgcttac ggctccagcg
    42541 cggataatcc gggttcggtt cgctgcacgc acgggagaaa acaattttgg cgttattgaa
    42601 atgcgcttcg cgtccgaggt taaaaccttc agcaaatggt ttggcaaacg cgacataaga
    42661 gatggtgccg ccataagcaa ggccacgcag cgccgattgc agcgcgtcgg cgtaaccgct
    42721 ggtttcgata atcacatcgg caccctgctt gccggtcagc gttttgatct cttttcctac
    42781 gtcagtgcca atggggttaa ggcagaaatc agcaccgtgg cgacgggcaa tatcacagcg
    42841 ataggcgata ggatcgacgc caatcaccac cgacgcgcca gcgcgtttcg ccagttggat
    42901 ggctatttga ccaatcgcgc caagccctac caccaccaca aaatcaccca cgcgcacgtt
    42961 ggcatcgcgc acgccgctca tggcaaactg cgccgggtcg taacaaacgg cgtttttcca
    43021 cgagctgcct tccggcattt tgcgcagctt gtagttattc accgcgttaa tgatgaccgt
    43081 ctcggagagc gggccgtagc cgcatacgct gtcgcccacc gcgtagtcgg taacgtcgct
    43141 gccgcactcg ataatgtcgc caaccaccat gttgccaagc tggaatttgc caaactcaat
    43201 gccgcgcggc gcatctgccg gacgcggagt gaacatctgc cattcgccgt taaagtcttc
    43261 atcgataaac gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt tcggtgcgcc
    43321 gaaacgcacg cggattttca cttcattggc taaaatcgcc cgatcttcat actcaaccag
    43381 cgcagcaaca cgcggtgctg tggctactaa ctttttcatt taaaactcct tgtaaagaaa
    43441 ctggccgctt tagcccttca cgccaccggc ggtcaaacca ctcttaataa aacgttccga
    43501 aagcgcatac ataatcacca ccggcaatgc ggtcaccagt gaggccgcca tcatccgtcc
    43561 ccagatgtaa tctggcgtac tgaacagcgc gttcaggccc accggtaagg tgaaattgct
    43621 ggcgctggag aggaaaatcg acgcaaacag atagtcgttc cacgccacca taaagcagta
    43681 gacgaacacg gatatcagcc cggacatcgc caacggcacg gtgatgcgga agataatttg
    43741 cagacggttg aggccatcca tcatcgccgc ttcttcgatc tcatcgggga tggtgtcgaa
    43801 gtagcttttc agcataaaca cggcggtggg cagagtttgc gtcaccatgg tgatgatcag
    43861 cgccatctcg gtgtcgtaaa tgcccagcgc ggtgatgatt ttgaacagcg gcaccaccag
    43921 caaaatgcca gagaacatat acaccgtgta aaagctggca ttgatggtca tccgaccttt
    43981 aaagcgcagg cgggaaagcg cgtaagcccc aagaatgccg aggaataccg ccaccacgga
    44041 tgaaaccacc gacaccacca gactgttacg gaagtagtcg acaaacggga aaatcatcgg
    44101 gttaaaaatg tcgacgtaat gctccagcgt ccactgctgc ggcagcagcg taggatgcag
    44161 tgagatcgcc tctttcgcgc ccttgaacga ggtcatcagc atcacaaaaa atggaaacag
    44221 cgtgatgatg agaaacagcg ccagcccgca gtaaaaaccg atgcgactga gtgtgcgttt
    44281 atttgttgcc attgaggttc acccgtttcc tggtcagcag aatgacagcg aaaataatga
    44341 tgaagagcac caccgagatc gccgccgctt ttcctaaatc attaaaggca aaggcggttt
    44401 tgtagagata tacaccgaga atatcgacct tggtcgtcag caaataaaca tccgcgaaca
    44461 tgtagaacat ccagatggtg cgcagggtca ccaccgtcgc caggaccggc ataatcgcgg
    44521 gcagcgtgac gatacgaaac cgttgccagg cattagcgcc atccatctct gccgcttcgt
    44581 acagcgattt gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca tacgggaagt
    44641 agcgccagat ggcgaacagc accaccagca caaagctact gcccggatta tcgaaccaca
    44701 gcggtgcctg ctcatagaga tgcagaagat cgacgccgag gtagttcaca atgccgtagc
    44761 cgttgttgaa catgtatttc caggcaaaca ccagcgaaat ggacggcgtt acgtaggaga
    44821 ggatcaccag cgaacgcgcg gttttgcgca gacggaactc gcggttgaaa aacatcgcca
    44881 ctgccagccc gagcacggtg ctgcccgcca ccaccagcgc ggtataccag accgtcatcc
    44941 acagcgagtg ccagaagccg ggatcggaga ggatacgcac atagttactc accccaacaa
    45001 aggttgactc gatgttggga ttgagcggca gacgtaaaaa actgatttcg atattcgaca
    45061 ccattggcca ggccaccaga ccacccagca gtaataagct gggcgcgaga agcagcagcg
    45121 caaagggcat atcggaacga cctgaaaaaa gcctgttcat tgcggattcc tgtcctgggt
    45181 ttagtgctgt tcgatcagtt catccagctt tttctggctc gcttgcagcg tgccagggag
    45241 atcggcttta cccacggtga cgttatgcac catggagctc accacgccag accccgtcac
    45301 atcacccatg cgggtaaagt ttttatcccc tactgcacca aagacctgaa tatttggcag
    45361 ttcaccgatt agctgattcg gtagttcacc cagcgcctta ataacgtcgt tgtctttcca
    45421 ggtggcggta gtgacaaccg ctttattcac cggcagcgca gcacctggcg acatcatcac
    45481 ccagtcggca atgttgtctg cctgctccat aaaggtgaca aatttttctg ctgcttccgt
    45541 ctcttcggtc ttttgcccgg cggtaatggt cagcgaggtc aacatgccgt agaccgcaga
    45601 gtttttctcg gttggcacca cgaaaccgac gtttttcggg tcgccttctt taatcacagc
    45661 cggaaggata taggtggagt aaatcgccat cggcgcggtg ccgttcataa aggcgtcttt
    45721 cacttccatg atgtcgttag aacccggcat agtgttggca gcaaggtcgc ggtaataggt
    45781 cagtgcctgc atcatctctg gtgtatcaag ggtgattttg ccttcggcgt taaagacgtt
    45841 agcctggttg gataacgcaa actgggagaa ggattgttcc gtcaacacgc tttctgctgt
    45901 aggcagcgca atgccgtatt ttttattcgc cgggtcattc agtttctgtg caacgtccag
    45961 cagctgttgc cagtttttcg gctcctcaag ccccgctttt gccagcacat ctttgcgata
    46021 ccagataccg ccaatccagg cgctgacagg aacaccggtc catgcgctac catcttcggt
    46081 acgcacaata cgcagtacgc catcgtaaaa cgcgccttca ccaacgttgc tgatgactgt
    46141 ggcaaccgct ttgcgatcga taagctgctc tttgtccatc actttggcgt agtcatggct
    46201 ggtttctatc acttccggca gcgaaccgct acgtgaaaga gtaatgactt tagtgttata
    46261 ggcatcttct tccacgggca cttgcttaac ggtgatgcca gggttttctt tttcaaaacg
    46321 ggcaatcaat ttactgataa cggcctggcg ctcctgctcc accgaagaat gcataaattc
    46381 gatggataca ttcgttttat tttcttcttt acaccctgaa atcagggcgc aggaaaccag
    46441 tgctgataac agcacgattt ttgatttaat catgagatgt tcctttttat ttaatccaca
    46501 taacctgcca ggggcgcagc gttatttcat tacccaatat atcaacttca ctaatcagat
    46561 cccgaccatg cagattagta atatttacat gctgaatatt tttactgaca ttaaacaatc
    46621 cagtcagaca attaccatca gcgttacttc tttgaatacg cattacggat gaattaatcg
    46681 tatcgatggt aaaattatta tccggatgaa actcgttatg gctgcgacga agtgtaatta
    46741 aacgcgacaa ctcatgatat accgcatgcc ttaatgtagc ttcatcgttc agttctcggg
    46801 ttatctcttt actgtgatat tttttacggt taatcgcacg gttatatccg agtttttcga
    46861 cacctgcgta atcattacgc gagccaagaa tactttgaat atatatcgct ggcacaccgg
    46921 ggaaacttaa caaaatcgca tgggcgagaa taaacctggc gcaacgttct tcatcgctac
    46981 tctcacggcg gcttaacgca tccatatagg tcacatttat ttcatacgga ctgcgtgtac
    47041 cgtcgggatt atttttccag tttactaatg caccttcctg ctgtaacgcc tcgaccagct
    47101 ctaatatttc gctttcaggc aacaagcccc gtagcgggtt tagcccgatg ccatcgtgag
    47161 aggcgaggaa gttaaaccag gtggtgttgc tggaaggtag tgtcaggttt tgcgcccacg
    47221 cacaaagcgc ctcaacgttc tgtttttgca ccgcatgcag caccagtggc ggcagcgaga
    47281 actggtacac catatgtgct tcgtcatcgc ctgcgccaaa gtaagcaatg ttgtctttat
    47341 gcggaacatt ggtctcggta atgatcactg tacctggcgc aacgttatca ataatcgacc
    47401 gtaacagttt gataatcaga tgtgtttttt ccagatggat gcagcttgtt cccggctctt
    47461 tccacataaa gccaacggca tccaggcgga catactcagc acctttcgca aggtaacaga
    47521 gtaaaacatc caccatcgcc agcaacactt cagggctacg gtaattcagg tcaatttgat
    47581 cgtcactaaa ggtggtccat aaatggcgcg ttgaatgatc gcgcatctgg aatggcgtta
    47641 ataacggtaa cgcacgcggg cgagtgacgg cgctgagatc ggtttgcggg tcaacggcaa
    47701 taaaaaaatc ttcaaaacct ggatgctgtt gtaaatagtt tttaaatcat tcactttttg
    47761 ccgacatatg gttgcagaca aaatcaaaca ttaaatgact gcattcaccg agttgctgaa
    47821 tatcctgcca ctcccccgct tcactggcga cctgatgata atcaattacc gaaaagccat
    47881 catcagatga ccatggataa aacggcaaca aatgaacatg tgaaaaaatg ctttgcagcc
    47941 attgatggta aaactgatta aatgtgggta atggttttaa atcattgctg tgaaattgat
    48001 cggcataggt aattaacacg acatcacttt catcccagtg ttttttacgt cgctgtgtaa
    48061 ttaatcgttt cgcactctca agacgcgtta caagtttctg taaatgagtt gcggtaaatg
    48121 ttccaccgta gatttcgtcc aggtaatccg taattttctg tttcatgttg cctttccgtg
    48181 gtagcggtcc catgaaacga gagactactc cgcagaaaat aaactgtcaa ttgagcacaa
    48241 gggttttagt cacgcaggaa atcatttttt accagggaaa aagcgtattt gtgagctgac
    48301 gcaaataatc tatggggatg taaataaggt gtgacagaaa aaaaacggcg cataagcgcc
    48361 gctcatggtg aattctttta acctttgacc ttcggcattg cgatgtcttt caggccaccg
    48421 gcgttctcaa cgtgggtata tcccatctcg ctaaggatct cttttgcttg ccctgactgg
    48481 cgtccggcat tgcaatacac tttcacggtg tcgtttttat ccggaacggc ggtggcaatg
    48541 cgctctttca cttccttcag gggaatattg atggccccct gaacgtgctc ttgctgatac
    48601 tgctctggaa cacgaacatc gatccagtgt tcagcggcga aaacgggcag tgaaaacacc
    48661 agtgccagag caagtaagcc ttttttaaac atgtaagcgt ccttctatgg atgaaaattg
    48721 ccatccatta tacgcctgag atttaacaat ttttgcgaaa ctcccctcac cttttataac
    48781 gctgtgccag tttattagca gcccgactta acagcggttc cagcgccaca gccagcaaca
    48841 ttttcagcgg tcggcgagca actgatttta tcgcccagcc cgctacaccc gccgggccat
    48901 agcgcagtgc ggtaagaagt accagcttgc ctgctaattt gaaaccaggc tttacctttt
    48961 gcccggcctg ttgccagcga gtattcataa ctttcctcac agttgacgga aacggctacg
    49021 taacgtgaaa gtatcggaag tgacataacg ttccatctcg cgtaaacgcg tttcacttgc
    49081 cgccagttca cggtcgactt catccagcaa ttcgctgctg gaaggtagct gctcaccaaa
    49141 ggccatgttg tccggcattg gatcaagcgc aaatgacaaa atgatgtaag caaccagggt
    49201 aaacagcgcc agaccgaaga aaatcgacag caccaccagg atacgcacca gttttgccgg
    49261 tacatcaaaa tagttggcaa tcccggcgca gacgccgcgg accatgccct gctgtggaat
    49321 acgccataat tttttattga gattaatgcc cgccattagc gatccctcca gttcggatgt
    49381 tctgcatcaa gaatagattc cagcgcctga atacgttcgc gcatccgttt tgcttcatca
    49441 gccagttgcg ctaatcgctg ctgctcactt tgcgacaatt cactgcgacc agaacgattg
    49501 ctgtaatgca gccataacca gatcggtaaa acaaacagca caaaaatggt taacggaata
    49561 gccagaaata gcgcgctcat aagtactcct tagacgatga gcggcggcgc gttcggacgc
    49621 cgcctggatg ttattattga ttgtcttgct tcattttggc ttttaattgt gccagttgtt
    49681 cgctgattgc atcatcggct ttcagttcgg caaactgatc gtccagcgat ttttgtttac
    49741 cgaagctgtg gctttctgct tccgcttcca tctggtcaat acgacgttcg aaagattcga
    49801 aacgagccat tgcttcatcc agtttgccac tgtccagctg acgacgcaca tcgcgcgacg
    49861 agtttgccgc ctgatgacgt aacatcaatg cctgctggcg agcgcgtgtt tcgctcaatt
    49921 tgttttccag ctcaccgatc tctttcttca tgcgtgccag cgtgtcgtcc accagcgtca
    49981 cttcatgttc caggctctta atcagatcgg tcagtttctg tttttcaatt aacgctgcac
    50041 gtgccagatc ctctctctct ttcagcagcg ccagttcggc tttttcctgc cattcaactt
    50101 cacgcgccga cgcttgttca atacggcgag tcagctgttt cttttctgcc aacgcgcgcg
    50161 ccgaagtaga acgtacttca accagtgtat cttccatctc ctggatcatc agacgaacca
    50221 gtttctgtgg atcttccgct ttctctaaca gagcgttgat gttggcattc acgatgtcgg
    50281 caaagcgaga aaaaataccc ataatgttgt cctcttgatt tctgcgttca ggattgtcct
    50341 gctgaactgt taatacaatt tgcgtgccaa ttttttatct ttttgattta taaagatctg
    50401 attgaagaac caacagcaac atgccaggat gagttagcga attacactaa caagtggcga
    50461 atttcatcat ggcagaatac aaagataatt tacttggtga ggcgaacagc tttctcgaag
    50521 tgctggaaca ggtttcgcat ctcgcaccgc tggacaaacc ggtgctcatc atcggcgaac
    50581 gcggcaccgg taaagagctg attgccagcc gcctgcatta tctctcctcc cgttggcaag
    50641 ggccgtttat ttcccttaac tgcgcggcgt taaatgaaaa tctgctggat tccgaactgt
    50701 ttggtcacga agcgggggcg tttaccggtg cgcaaaaacg tcatcctggg agatttgaac
    50761 gtgccgacgg cggtacgcta tttcttgatg aactcgctac ggcaccgatg atggtgcagg
    50821 agaaattatt gcgcgtgatt gagtacggtg aactggagcg cgttggcggc agccagccat
    50881 tgcaggtgaa tgtgcggttg gtatgcgcga cgaatgccga tctcccggcg atggtcaatg
    50941 aaggcacttt tcgcgctgac ctgctcgacc gactggcttt tgatgttgta caactgccac
    51001 cactgcgcga gcgcgaaagc gacataatgt tgatggcaga acactttgcc atccagatgt
    51061 gtcgggaaat taagctgcct ctgttcccgg ggtttacaga gcgcgccaga gaaacattgc
    51121 tgaattatcg ttggccggga aatattcgtg aattgaaaaa cgtggtggaa cgttcagtgt
    51181 atcgccacgg caccagcgat tatccgcttg atgacatcat tattgatccc tttaaacggc
    51241 gtccgcctga agacgctatc gccgtttcag aaaccacctc gcttccaaca ctgccgctgg
    51301 atttacgtga gtttcagatg cagcaggaaa aagagttgct gcaactcagt ttgcaacaag
    51361 ggaaatataa ccagaagcgt gcggctgaat tactggggtt aacttatcat cagttccgcg
    51421 cgttgttgaa aaagcaccag atttagcgaa gttgagcaat acaacttgcc ggatgcggcg
    51481 tgaatgcttt attcggcata cataatcaat acgtcgtttg caggcctgat aagcgcagcg
    51541 catcagacat tattgcgttg ggctattaat cgctcagcgc atcctgcaaa attttcattg
    51601 ccgcatcgaa ttgcgcatcc ggtatggtca gcggatacag gaagcgaatc acgttgccgt
    51661 atgcgccaca ggtcagcagg agcagcccct gcgccagcgc gcgttgctgg attttctgtg
    51721 caatcgccgc tgacggctcg cccgtttgcg gatcgttaaa ctctgccgca atcatcgacc
    51781 ccaggccgcg taccgcagca atggccggaa cgctttcttt ggcatcaatc aacgtgtttt
    51841 tgagacgctg gcccagttga ttcgcgcgtt cgcagagtga ttctttgtcg ataatattga
    51901 gcaccgcgtg cgcggcagcc accgccagcg ggttcccggc gtaggtgcca cccaacccgc
    51961 ccggcgcggg cgcgtccata atattcgcgt taccgaccac gcccgaaagc ggcatcccgc
    52021 ccgcgaggct tttcgccatc gtcattaagt ccggcttatc ggcgtaatga tccatggcaa
    52081 acagtttacc ggtacgcgca aagccgcttt gcacttcatc ggcaatcatc acaataccgt
    52141 gctcgtcgca caggcggcga atggcggcaa ccagctcttt tggcgcaacg ttgaaaccgc
    52201 cctcgccctg caccggttcg aaaataatgg ccgcgacctg cttcgcttcg atgtctgatt
    52261 taaacaagcg ttcgatggcg tcgagggagt cctgtgttga aatgccgtgt aaatctgacg
    52321 gataaggtac gtgatacacc gaaccaggga acgggccgaa gccgattttg tacggcgcaa
    52381 cttttccggt cagcgccatg gtcatatatg tacgaccgtg gaagccaccg ctaaacgcaa
    52441 tcacgccagg tcgtccggta tgggcgcgag caattttcac cgcgttttcc accgcttccg
    52501 caccggtagt gaagaatgcg gttttagcct gcccgctcac tggggcaagg gcgttaattt
    52561 tctccgccag cgtgacgtag ctttcgtacg gcacaatctg atacgccgtg tgggtaaact
    52621 gttgcaattg ctgctccacc gccgcgacca gatcagggtg gcgatgcccg gtattcagca
    52681 ccgcaatgcc tgcggcgaaa tcgatgtatt cgttgccctc tacgtccttc agcgtagcgt
    52741 tttctgccga ctgggcgaag aagttacaca tcacgccaac cccgcgtggg gtagcagaaa
    52801 gacgacgctg atggaattca ttgttgctca tgtttcacac cttttgactc ttcactgtta
    52861 atcggaaatt aaacgcccag acgatcgcgc aggctgtaat aagccgcgcc catcgcggta
    52921 aatggcacgc gcagcgtgcg cccgccgggg aacgggtaat gcggcagatt ggcgaaggca
    52981 tcgaaacgtt cggcgtcgcc gcgcagcagt tcggcaatca gacgcccggc aagatgggta
    53041 caggtgacgc cgtggccgct gtagccctgc atgtaataga tgttggtatc aaggcgaccg
    53101 aactgcggca tgcgcgacaa ggtcagcagg aagttgcccg tccagcggta atcaattttc
    53161 acgcccttca gctgcgggaa ggttttcagc agtttcggca ccacaaggcg ctcaacgtca
    53221 tccgggtcgc gcgcgccgta gaccacgccg ccgccgtaca gcaggcggtt gtcggcggta
    53281 agacggtagt aatcaagcag atagttacag tcttccacac agtagttttt cgggatcagc
    53341 gaacgggcta aatcttccga cagccgttcg gtggtgatca cctgcgtgcc gcacggcatg
    53401 ctgcgtttcg ccagttccgg ctctacttta tcgcccagat acgcattccc ggcgacaatc
    53461 acatacttcg ccgtcacctg acctttggca gttcgcacaa cggctggcgt ggtgtgctgg
    53521 atctgcgtca cggcagaaag ttcatacacg cgcccgccgt tgaggcggat ggcgtccgct
    53581 tcgccaatgg cgaggtttag cggatgaata tgcccaccgc tgtgatccag cagcgcaccg
    53641 gtatagcgat cgctggctac ttcacggcga atggcgttgg cgtccagcaa ttccagctgt
    53701 ttattgccgt agcgttccca gttctctttc tgctcttcaa gtgtggcgag ctgtttatca
    53761 ttcatcgcca caaacaggcc gccggggcgg tagtcgcagt caatctgata acgtttgata
    53821 cgttcgcgga tgatctcacc accttcgaac atcatgctgc cgagcatacg ggcggtgtcc
    53881 atgccgtagc ttttttcgat cacgtcgatg tcgcggctgt aggagttcac aagctgcccg
    53941 ccattgcgcc cgcttgcgcc aaagccgatg cgtgaggctt cgagaaccac tacgtcaaag
    54001 cccgcttccg ccagatgcag cgcggaagag agcccggtat agccgccgcc gaccacgcaa
    54061 acgtcgcagg aaatcgactc attcagcgtg tcgaatggcg cgtatttatt cgcactggcg
    54121 gcgtagtaac tgctggtatg ttcggtcatt tcaggcctcc aggcttatcc agatggtttt
    54181 cagttcggtg aatttttcaa gggcatgcag ggatttgtcg cgaccgttgc cgctctgctt
    54241 atagccgcca aacggcacgg tcatatcgcc gtcgttgtag ttattgacga agacggaacc
    54301 agccttcagg cggcggctca tgcggtgcgc gcgggagagg tcgcgcgtcc ataccgccgc
    54361 gccaaggccg tactggctgt cgttggcaag ctgtagcgcc tggtcttctg atgtgaaacg
    54421 cgtgaccacc agcaccggac cgaaaatctc ttcgcggctt aaggacgcat tcgggtctac
    54481 atccacaaag atggtcgggc cgatggcggc agccagctca gcgttacgac catccagcag
    54541 cagttgccct ttgctttcgc cttcccgaat aaagctatgg accgagtcag cgtgagcgct
    54601 gtcgattaag gtgcccatgg tggttgcggg atcaagtgga tggcccggct gccagttttg
    54661 cgcctgctgt tttaacaggg ctaagaattc atcggcgatg ctctcttcca gtaacaaacg
    54721 cgttccggcg atgcacacct ggccctggtt gtagaagatg cctgcggcgg tggcgcttgc
    54781 cgctttttgc agatccggac aatcggcgaa aacgatgttg gcgcttttgc cgcccgcttc
    54841 cagccagacg cgtttcatgt tgctctcgcc cgcatctttc agcagctgtt tcccggtacg
    54901 ggtcgaaccg gtaaaggcaa tggcgtcgat atcgttatga cgcgacagcg cctgccccgc
    54961 ttcatgacca aaacctgtca ccacgttcaa aacaccatcc ggcaagcctg cttctttcgc
    55021 cagccccgcg agacgaatcg cactgagcgg tgatttttca gacggtttca ggacaacgct
    55081 gttccctgct gccagcgccg ggccaagctt ccagcaggtc agcagcagcg gaaagttcca
    55141 cggtacgatg gcggcaatca caccgaccgg ttcacgcacg atcatcgcca gctcatggct
    55201 actggtggtc gccacttcgc catacacttt gtcgatcgct tcggcgtacc agcgaatggc
    55261 gcgcgccgcg ccgggaatat catcacgcag actgtgacga atcggtttgc cggtgtcgag
    55321 agtttccagc agtgccagct cttcggcgtg ggcttccatt aaatcggtga gtttattcag
    55381 taccgcttta cgtttagccg gagaagagag tgaccagtcg ccgcgttcaa atacgccgcg
    55441 tgctgcgctc accgcacggt cgatatcgac gctcttgccg cgggcaattt tcgccagcgg
    55501 tgcctgggtg accggatcaa cggtttcaaa ggtttcattt tccgccgcag cagtatattc
    55561 accgttaata aataagcggg tttcaatggc gagacttaac gctttatcct gccagtaagc
    55621 cagatgatga aaattcatta tgactcctgt ttcacgtcta tcagatatat gcagtttgtg
    55681 aacgtcaggg cgcagagatc cccgggccgt atgcccgttt atttctggcg acattcttta
    55741 tatacacttc atccttcaag ccgcctctgc cttggctgcg ttcgctcccc cgaatcactt
    55801 acctgagtaa actcatcggg attcgctcac ttgccgcctt gatgcaactt gaatgatttt
    55861 gtgtatagat tgccgggcgc attgcgctta cccggcttac agaacaaaaa gattaaaacg
    55921 tggtgggcgt atgggcgctg ataattcggc aaatacctgc cgacgtatta ctgaaactgt
    55981 gcgggatgcc ggtattaatg gcataacttt gccccgcgac gaggtggtaa tcctgaccat
    56041 taatcgtcag aacaatttca ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa
    56101 ttctttcccc agtggttgtg cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat
    56161 tcgggttacc gttatgaacc agcttcattg acacaccctg actgcccatc tcaattaagt
    56221 cgtcctgatt aatgacgacc tgcggctcat caggtttttc cggctcggaa aagaattccg
    56281 agagtgacag accatacacc ttcagcagct tttgcagcgt actgatggca gggctgactt
    56341 tatcttgttc tatcgtactg atagcactgt gagtcagccc ggagagttcg gcggcacgac
    56401 gttgtgaaag cccctgctgc tggcggattt ccgacaagcg ttttcctggc gccagtccct
    56461 catcactcat atttgcattt ccttaaactg tagtggtcag agtcgctgtt tttcagcgat
    56521 atggtgctga caagcggtga taaagccctc gaacaatata cgcgaaagcg cgtactcgct
    56581 actgttccat tccgggtgcc actgtacgcc cagcgcaaaa ggatgattga tgacgctaac
    56641 cgcctcaact aagccatccg gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt
    56701 cgccccttgc ccatgcagag agtttaccca aaagttgcta cattcaggta acaacgcaga
    56761 cagtaatccc ccctcttcaa cctgaacttc atgcgacggt gcatattgct gttccaccgg
    56821 cagttcggga tcttcccgat gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa
    56881 cgaaccaccg gttgccacca ccagttcctg taaaccccgg cagatggcga aaatggggat
    56941 gcgcctttcg agtgcggcat taattaacgc catgctcaga agatcacgcc cgggatcggc
    57001 gtcaggctca tcgccgtttt caccatatag gtgcggctgc acattgctgg gactaccagg
    57061 aagataaatg ccatcgagtt tcggcaaaag ttgttcaagt aatgacggtt ccgccagcgc
    57121 atgtggtagc gcaattggca agccgcctgc gtggatgatg gcattcaggt acttttcttg
    57181 cagagtctgg gtcgcatgac ccttaagcct gttcctgcac attacgacac cgataaccgg
    57241 attgttcatt atattttcca tgctcaatct cacaaagtgg actaaattat cgccattact
    57301 gcggtttaaa tgacctttat gttcaatatt ttttcaatct agcagtgggt tttcattttt
    57361 gcaaactcaa tttaacattt gacaaacatt tagtttgcat acagattcga atggtggtca
    57421 ttatatttta cgctttgata acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac
    57481 caatatcgtt gaagtagaga actttgttca gcagtcagaa gagaggcggg gtagcgcctt
    57541 tacgcaggaa gtgaaacgct acctggagcg ctacccgaat acgcaatatg ttgatgtttt
    57601 acttactgat ttgaatggtt gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa
    57661 gttggagaaa gggtgttatt tcccggcctc ggtgtttgca atggatattc tgggcaacgt
    57721 agtagaagaa gcgggtctgg gtcaggaaat gggcgagccg gatcgtactt gtgttcctgt
    57781 tctcggttcc ttaacccctt ctgccgccga tccagagttt atcggtcaga tgctcctgac
    57841 catggtcgat gaagatggcg ctccctttga cgttgagccg cggaacgttc ttaaccgcct
    57901 gtggcagcag ctacgccagc gcggattgtt cccggttgta gcggtagagc tggagttcta
    57961 tttactggat cgtcagcgcg acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac
    58021 cgatgaccgc aatacacaaa gccaggttta ctccgttgat aacctcaacc acttcgccga
    58081 tgtgctcaat gatattgatg aactggcgca gttacagtta attcccgccg acggtgcggt
    58141 cgccgaagcc tcgccgggtc agtttgaaat caacctttac cataccgata acgtgctgga
    58201 agcctgcgat gatgcgctgg cgctaaaacg cctggtgcgt ctgatggcag aaaagcataa
    58261 gatgcacgcc acttttatgg cgaagccgta tgaagagcac gcgggcagcg gaatgcatat
    58321 ccatatcagt atgcaaaata accgtggcga gaacgtgctt tctgacgcgg aaggcgaaga
    58381 ttcgccgctg ctgaaaaaga tgctcgccgg gatgattgac ctgatgccgt cgtcgatggc
    58441 gttgctggca ccaaacgtga actcgtatcg ccgcttccag ccgggaatgt atgtgccgac
    58501 gcaggcgtcg tggggccata acaaccgcac cgtcgccctg cgtattccgt gcggcgaccg
    58561 tcataatcac cgcgtggaat atcgcgtggc gggtgccgat gccaacccat atctggtgat
    58621 ggcagcgatt tttgccggta ttttgcatgg ccttgataac gagctgccgt tgcaggaaga
    58681 agtcgaaggc aacgggctgg aacaggaagg cttacccttc ccgattcgcc agagcgatgc
    58741 cctgggtgag tttatcgaga atgatcacct gcgccgctat ttaggcgaac ggttttgcca
    58801 tgtgtatcac gcctgtaaaa atgatgagct gttgcagttt gagcgcctca tcacagaaac
    58861 agaaattgag tggatgttga aaaacgcgta attccccatg cggggttgtt tcatcggcct
    58921 gagcggagcc aggcctgaca tttgctggta atccagacat ttcccggatg tcgcgttatg
    58981 gagcgcaggc ggcaacgggc ttccgttaac gattaagact gtgacgagga aatgagatga
    59041 tgccgatgat taaatccccg catggcgagg gaggctgcgt atgcgcccct cctgctactg
    59101 actggactcc gccccctttg cttcccttgc taaacaggtt tgatttcagg tcgacacgac
    59161 cgcaaacctt attacgcagg ggaggcagca attatggcta ttaattcacc actgaatatt
    59221 gctgcgcaac ccggcaaaac ccgtctgcga aaatcactga aattgtggca ggtggtaatg
    59281 atgggtctgg cctatctcac gccgatgacc gtatttgata cttttggtat tgtctccggc
    59341 attagcgacg gtcacgttcc agcgtcctat ttgctggcgc tggcgggcgt gctgtttacc
    59401 gctatcagct acggcaaact ggttcgccag tttccggagg ccggttcggc ctatacctac
    59461 gcgcaaaagt cgattaatcc gcacgtcgga tttatggtcg gctggtcatc actgctggat
    59521 tatctctttt tgccaatgat caacgttttg ttggcgaaaa tctatctctc cgccctcttc
    59581 ccggaagtgc cgccgtgggt gtgggtggta accttcgtcg ccattttaac cgccgcgaat
    59641 ctgaagagcg tcaacctggt cgctaacttc aataccctgt ttgtactggt gcaaatctcc
    59701 atcatggtgg tgtttatctt cctggtggtt cagggactgc ataaaggaga aggcgttggc
    59761 accgtctggt cacttcagcc gtttatcagc gagaacgcgc acctgatccc gattattacc
    59821 ggtgcgacga ttgtctgttt ctcgttcctc ggtttcgatg cggtgaccac gctttcggaa
    59881 gagacgccgg acgccgcacg cgtgatcccg aaagccatct tcctgacggc ggtatacggc
    59941 ggcgttatct ttatcgcggc gtcgttcttt atgcagctgt tctttcccga tatcagccgc
    60001 tttaaagacc cggacgccgc actgcctgaa attgcgctgt tcgtcggcgg caagctcttt
    60061 cagtcgattt tcctctgcac cacgtttgtg aacacattag cgtctggcct ggcttcacat
    60121 gccagcgtgt cgcgtctgct gtatgtgatg gggcgtgaca atgtgtttcc ggagcgcgtg
    60181 tttggctatg tgcacccaaa atggcggact ccggcactca acgtcattat ggtcgggatt
    60241 gtcgcgctgt cggcgctgtt ctttgattta gtcaccgcga cagcactgat taacttcggt
    60301 gctctggtgg cgtttacctt cgtgaatctg tcggtgttta atcatttctg gcggcgtaaa
    60361 ggaatgaata aaagctggaa ggatcacttc cactatttgc tgatgccgct ggttggcgcg
    60421 ctgacggtgg gtgtgctgtg ggttaaccta gagtcaacgt cactgacact cggtctggta
    60481 tgggcttcgt taggcggcgc atatttgtgg tatctgatcc gccgctatcg caaagtgccg
    60541 ctgtacgaag gtgacagaac gccggtgagc gaaacgtaat agccggatgc gcggcgcatt
    60601 atcctgcctg agggtaacgc gccgcgccat taactataaa aagatgtatg acccggatca
    60661 cgagtgctat catccgccct tatgcttagc gcattaaccc caggggcaaa taatgaacca
    60721 cgatatcccg ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc
    60781 tgtcgcagag gctgaacgca caccgctggc ccactacttc cagttactgc tcacccgttt
    60841 gatgaacaat gaagagatca gcgaagaagc ccaacatgaa atggctgctg aagcggggat
    60901 taatcccgta cgcattgatg agatcgcaga gtttttgaat caatggggta atgagtaatt
    60961 atttaattgc agacgatcgg tcagtatcgt tgtccggata aagcgttcac gccgcatccg
    61021 gctaacaatg acacgcactg cagcgtgctt taatgggttt ctcacgatcg taccgctctg
    61081 ctcctcgcta agttgttctg tgagttatag ttatagagaa atcacgggta cgaatgttga
    61141 acagatggcg acgccacctg ttcaacgacc aaccacacgg ccctcattac gacccctgaa
    61201 cacaaacggt aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc
    61261 ccttaatatt atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg
    61321 tcaggcaatc gccgcgcctg aatccccccc gcatgctgat atccgcgaca gcggttttgt
    61381 ctattgcgtc agcgggcaag tcaacacctt taacccatcc aaagcgagca gtgggttaat
    61441 tgtcgatacc cttgccgccc agttttatga tcgactgctg gatgtcgatc cctataccta
    61501 tcgcctgatg ccggaacttg ccgaaagctg ggaagtactc gacaacggcg cgacctatcg
    61561 cttccacctg cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa
    61621 aatgaatgcc gacgatgtgg tgtttacctt ccagcgaatt tttgaccgca acaacccgtg
    61681 gcataacgtc aacggcagca acttccccta cttcgacagc ctgcaatttg ccgataacgt
    61741 gaaaagcgtc cgcaaactgg ataatcatac cgttgagttc cgactcgccc agccggatgc
    61801 ttcttttttg tggcacctcg caacccatta tgcttcggtc atgtcggcag aatatgcccg
    61861 gaagttagag aaagaagatc gccaggagca actcgaccgt caaccggtcg gcaccggtcc
    61921 gtatcagttg tcggaatacc gcgccgggca atttattcgc ctacaacgtc atgatgactt
    61981 ctggcgcggt aaaccgttaa tgccgcaggt agtggtggat ttaggctccg gcggcaccgg
    62041 acgtctgtcg aaactcctga ccggggaatg cgacgttctg gcctggcctg ctgccagcca
    62101 gctatccatt ttgcgtgacg acccgcgctt gcgtttaacg ctgcgtcctg ggatgaacgt
    62161 cgcctatctg gcatttaaca ccgccaaacc gccgctaaat aatcccgctg tccgccatgc
    62221 actggcactg gcgattaata accagcgcct gatgcaatcc atctattatg gtacggctga
    62281 aacggcggcc tctattttac cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac
    62341 tgaatacaat ccggcgaaat cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac
    62401 gctgaaactg tgggtgccca cacgttcgca ggcgtggaac cccagtccac tgaaaactgc
    62461 cgaactgatt caggcggata tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga
    62521 aggtcgcttt caggaggcgc ggttgatgga tatgagccat gatctgacgt tatccggttg
    62581 ggcgacggac agtaacgacc cggacagttt cttccgtcct ttactgagct gcgcggcaat
    62641 tcattcacag accaacctcg cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa
    62701 ggcgctctcc tcgcagcagc tggcggcgcg tattgaagcc tatgacgaag cgcagagtat
    62761 tctggcgcag gaattgccca ttttgccgct ggcgtcgtca ttgcgtttgc aggcctatcg
    62821 gtacgatatc aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta
    62881 tcgcgagaaa caggatgagg tgaaaaaacc atgattatct tcaccttacg ccgcattttg
    62941 ttattgattg tcaccttgtt cctgctgacc ttcgttggct tcagcttgag ctatttcacc
    63001 cctcatgcgc cgttacaagg cgcgtcactg tggaatgcct gggtgttctg gtttaacggc
    63061 ctgatccact gggattttgg tgtttccagc attaacggtc aaccgattgc cgaacagtta
    63121 aaagaggtct tcccggcgac gatggaactg tgcatcctcg ccttcggttt tgcgctgatt
    63181 gttggcatcc ccgtggggat gattgccggg attacgcgcc ataagtggca ggataacttg
    63241 atcaacgcca tcgccttact gggcttttca atcccggtgt tctggctggc gcttctgttg
    63301 acgctgtttt gttcactcac gctaggttgg ttgcccgttt cagggcgttt cgatctgctc
    63361 tacgaagtga aaccgattac cggttttgcg ttgattgatg cctggctttc ggactcacca
    63421 tggcgggatg aaatgatcat gagcgcaatc cgccatatga tattgcccgt gatcaccctg
    63481 tcggttgcgc caacaactga agtgatccgc ctgatgcgta tcagcactat cgaagtgtat
    63541 gaccagaact atgtaaaagc ggcggcgacc cgcggtttgt cgcgctttac tattttgcgt
    63601 cgccacgttc tgcataacgc gttaccgccg gttattcctc gcctgggctt acagttttcc
    63661 accatgctga cgctggcgat gatcaccgaa atggttttta gctggccggg tttagggcgc
    63721 tggttaatta acgccatccg ccagcaggat tacgcagcca tttccgccgg agtgatggtg
    63781 tgtggctcac tggtcattat tgttaacgtg atttctgata ttttgggtgc catggctaac
    63841 cctctgaaac ataaggaatg gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc
    63901 gggcacgctg cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct
    63961 gtacggctgc gcggggctgg ctgtactgtg tatttttggc ggctggtttg cgccttacgg
    64021 catcgaccag caatttctcg gttatcaatt attgccgccg tcatggtcgc gctatggcga
    64081 agtttctttc ttcctgggga ctgacgacct cggacgcgat gttttaagcc gtttactgag
    64141 cggagctgcg ccaaccgtgg gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg
    64201 cctggtactg ggcaccttcg ccggggcgac acacggcctc cgctcagcgg tgcttaacca
    64261 tattctggat accttactgg cgatcccttc gctgctgttg gcaattatcg ttgtagcgtt
    64321 tgccggaccg agtttgtccc acgctatgtt tgctgtctgg ctggcattgc tgccgcgtat
    64381 ggtacgttcg atttacagca tggtgcatga cgaactggaa aaagagtacg ttatcgccgc
    64441 ccgtctggat ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc caaacatcac
    64501 cgccgggctg gtcaccgaga tcacccgcgc actgtcgatg gcaattctcg atatcgccgc
    64561 gctgggcttt ctcgatctcg gcgcacaact cccctcgcct gaatggggag cgatgctcgg
    64621 tgatgcgctg gaactgattt atgtcgcccc gtggactgtc atgctgccag gtgcggcaat
    64681 tatgattagc gtgttgttgg ttaacctcct tggcgatggc gttcgtcgtg cgattattgc
    64741 gggggtggaa taatgccatt actcgatatt cgtaacctga ccattgaatt taaaaccggt
    64801 gatgagtggg ttaaagccgt cgaccgggta agtatgacct taaccgaagg tgaaatccgc
    64861 ggtcttgttg gtgaatccgg ttccggcaag agtttgattg cgaaagcgat ttgtggggtg
    64921 aataaagata actggcgtgt tactgctgac cgtatgcgtt ttgatgatat cgatttgctg
    64981 cgtctctccg cacgcgaacg gcgcaaactg gttggccata acgtgtcgat gattttccag
    65041 gaaccgcagt cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc
    65101 ccagcctgga cctataaagg ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc
    65161 attgaactgc tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg cagttttccc
    65221 tatgagttga ccgaaggtga atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa
    65281 ccgcgtctgc tgattgctga cgaaccgacc aactcaatgg agccaacaac ccaggcgcaa
    65341 atctttcgcc tgctgacgcg tctcaaccaa aacagcaata ccactatttt gcttatcagc
    65401 catgacttac aaatgcttag ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa
    65461 acagtggaaa ccgcgccgag taaggagttg gtgacgatgc cacatcatcc ttatacccag
    65521 gcgctgatcc gcgcgatacc agacttcggc agcgcgatgc cgcataaaag tcgcctcaat
    65581 acgctgcccg gcgctatccc actgctggaa cagttaccga ttggttgtcg tctggggcca
    65641 cgttgcccgt atgcacaacg agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat
    65701 catctctatg cctgtcattt cccgctgaac atggagaaag agtgagatga tcgaaacgct
    65761 gcttgaagtg cgtaatttaa gtaaaacgtt ccgctaccgg accggctggt ttcgtcgtca
    65821 gaccgtagaa gcggtaaaac ccttgagctt tacgctacgt gaaggccaga cactggcgat
    65881 tattggcgag aatggttcgg gtaaatccac gctggcaaaa atgctggcgg gaatgataga
    65941 gcctaccagc ggcgagttat tgattgacga tcatccactg cattttggcg attattcctt
    66001 ccgcagtcag cgtattcgca tgatttttca ggatccttcg acctcgttga atccccgtca
    66061 acgtatctcg caaattctgg attttccact gcgcctgaac accgacctgg aaccggaaca
    66121 gcgtcgcaaa cagattattg aaacgatgcg tatggttggg ctgctaccgg atcacgtcag
    66181 ttactatccg catatgttgg cacccggaca aaaacagcgt ctgggtctgg cgcgcgcgct
    66241 gatattgcgc ccaaaagtca ttattgccga tgaagccctc gcttcactgg atatgtcgat
    66301 gcgttcgcag ttgattaatc tgatgctgga gttacaggaa aaacagggca tttcgtatat
    66361 ttatgttacc cagcatatcg gaatgatgaa gcacattagc gaccaggtgc tggtgatgca
    66421 tcagggcgag gttgtcgaac gaggcagcac cgcggatgtg ctggcatcgc cgctgcatga
    66481 actcaccaaa cggctgattg ccgggcactt tggcgaagca ttgacagcgg atgcgtggcg
    66541 taaagatcgc taattttctg aatcctcctt ccagattatc cacctcattc tgacaagaga
    66601 ttaaatcttg tcagaatgag gtgatggata aaataaaatc aaatgcgcgt gatttaagac
    66661 gcaatctcac tttgcaggaa cgaaagctct ggcgatatct tcgcagccga cgttttggtg
    66721 atttcaaatt tcgccgtcaa catccggtgg ggagctacat tctcgatttt gcttgctgct
    66781 cggcgcgcgt agtcgttgag ctggatggtg ggcagcatga tttggcagtt gcctatgata
    66841 ccaggcgcac tagctggctt gagtcgcaga gctggaccgt gctgcgtttc tggcataacg
    66901 agattgattg taatgaggag gcggtgctgg agattattct gcaggaactg aaccgccggt
    66961 caccctcacc ctgaaagggc gagggggccg accgagccga atagctgttg tggtgaaaac
    67021 atggagacag tgctggagaa tattcggcaa ggtctgaacc atcccagcca tcgccataaa
    67081 agggttaggg gctgtatgag cctgtttgtt gctggggtaa caatatttgc acaatatggt
    67141 cccctcgccc ctctggggag agggttaggg taaggggaaa agcgcccacc ctgccgcagc
    67201 ctgctccggt cggacctggc aactatagct actcacagcc aggttgatta taataaccgt
    67261 ttatctgttc gtactgttta ctaaaacgac gaatcgcctg attttcaggc acaacaagca
    67321 tcaacaataa ggattaaagc tatgggtttt ctttccggta agcgcattct ggtaaccggt
    67381 gttgccagca aactatccat cgcctacggt atcgctcagg cgatgcaccg cgaaggagct
    67441 gaactggcat tcacctacca gaacgacaaa ctgaaaggcc gcgtagaaga atttgccgct
    67501 caattgggtt ctgacatcgt tctgcagtgc gatgttgcag aagatgccag catcgacacc
    67561 atgttcgctg aactggggaa agtttggccg aaatttgacg gtttcgtaca ctctattggt
    67621 tttgcacctg gcgatcagct ggatggtgac tatgttaacg ccgttacccg tgaaggcttc
    67681 aaaattgccc acgacatcag ctcctacagc ttcgttgcaa tggcaaaagc ttgccgctcc
    67741 atgctgaatc cgggttctgc cctgctgacc ctttcctacc ttggcgctga gcgcgctatc
    67801 ccgaactaca acgttatggg tctggcaaaa gcgtctctgg aagcgaacgt gcgctatatg
    67861 gcgaacgcga tgggtccgga aggtgtgcgt gttaacgcca tctctgctgg tccgatccgt
    67921 actctggcgg cctccggtat caaagacttc cgcaaaatgc tggctcattg cgaagccgtt
    67981 accccgattc gccgtaccgt tactattgaa gatgtgggta actccgcggc attcctgtgc
    68041 tccgatctct ctgccggtat ctccggtgaa gtggtccacg ttgacggcgg tttcagcatc
    68101 gctgcaatga acgaactcga attgaaataa tcattctgtt ggtaaatatt gggcggcgtt
    68161 ctgccgcccg ttatctctgt tatacctttc tgatatttgt tatcgccgat ccgtctttct
    68221 ccccttcccg ccttgcgtca ggataacgat ttcctttacg accaaggagc gcccatggaa
    68281 caacgccaca tcaccggcaa aagccactgg tatcatgaaa cgcaatccag tactgcggag
    68341 tatgacgttc tgcctctggt cccggaagcc gcaaaggtca gcgatccctt tctgctcgac
    68401 gtgatccttg atgaagaaac gctggctccc ttcctttcat ggctggtccc tgcgcgcgtt
    68461 cttgcagtgg aattgttccc tgaccagctt accgtgaccc gttcacagac cttcaccgct
    68521 tatgaacgct tgtctacggc cctgacggtt gctcaggttt gcggcgtcca gcggttatgt
    68581 aactactatt cggcgcgact tacgccgctc cccgggcctg attcctccag ggaaagcaat
    68641 cataggttgg cacaaatcac gcaatatgcc cgccaactgg ctagctcgcc ttctattatc
    68701 gacaaccgat cgcgccagca tctgaatgac gtcggtctta ctgccgggga ctgtgtgatc
    68761 attaaccaaa tcattggttt tattggcttt caggcgcgga ccattgcgac atttcaggct
    68821 tatctcgggc acccggtacg ctggttaccc ggtctggaaa tacaaaacta cgccgacgcg
    68881 tcactgtttg ctgatgaatc aatacgctgg cgaagcagct atgaggtgga aaaactacct
    68941 gaagagtaca caaaaagttc aacagcagaa ctttgccaac tggccgaaac actctctctc
    69001 caccctattt cactttccct tctcgaaagg ttgttaaaca gtacacgggt taatacacag
    69061 ccggataata agcttgcggc gttgttatgc gcgcggataa atggcagtcc tgcttgtttt
    69121 gccgcctgta tggattcatc aaatgaatat aaaaaaatca gcccccttct gcgcaagggc
    69181 gaaaatgaaa ttaaccaatg ggctgaccgt cattctgttg agcacgctac cgttcaggcg
    69241 atacaatggc tgacccgagc acccgatcgc tttagcgccg cccagttcag ccctttactc
    69301 gaacacgaaa aatcatcaac gcagattatt aatctgctgg tatggagcgg gctgtgtggc
    69361 tggataaatc gcttaaaaat cgcgttgggt gagacatatt aaccttgccg cgtcagacag
    69421 attcgcgtaa aactgtcagc cgctctaatg gccaccaaaa tagacaatta tgtttcagga
    69481 caacccgctg ctagcgcagc ttaaacagca actgcattcc cagacgccac gcgctgaagg
    69541 ggtggtaaaa gccacagaaa aaggctttgg cttcctggaa gtcgacgcgc aaaaaagtta
    69601 tttcattccg ccgccgcaga tgaaaaaagt catgcatggc gaccgaatta tcgcggtgat
    69661 ccacagtgaa aaagaacgtg aatccgcaga gccagaagaa ctggttgaac cgttcctgac
    69721 tcgtttcgtg ggtaaagttc agggcaaaaa tgaccgtctg gccatcgttc ctgatcatcc
    69781 actcttaaaa gacgccattc cttgccgcgc agcccgtggc ctgaaccacg agtttaaaga
    69841 aggcgactgg gcggttgccg aaatgcgccg tcatccgctg aaaggcgatc gttctttcta
    69901 tgctgaactg acacaataca tcacttttgg tgacgaccac tttgtaccgt ggtgggttac
    69961 ccttgcgcgc cataatctgg aaaaagaagc accagacggc gtcgctaccg caatgctcga
    70021 tgaaggtctg gttcgtgaag atctgatcgc gctggatttt gtcaccatcg acagtgccag
    70081 cacagaagat atggatgacg cccttttcgc taaggcgttg ccggatgaca aacttcagct
    70141 gattgtggcg attgccgatc caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc
    70201 cgcgaaaatt cgcgcattca ccaactatct gcctggcttc aacatcccta tgctgcctcg
    70261 cgagctttct gacgatctct gctcactgcg cgccaatgaa gtccgcccgg tactggcatg
    70321 ccgcatgacg ctctccgctg atggcaccat tgaagataat atcgaattct ttgccgccac
    70381 catcgaatcc aaagcaaagc tggtgtatga ccaggtttct gactggctgg aaaataccgg
    70441 tgactggcag cctgaaagtg aagcaattgc cgaacaagtc cgtttgctag cgcaaatttg
    70501 ccaacgccgc ggcgagtggc gtcataacca cgcactggtg tttaaagatc gcccggatta
    70561 ccgctttatt ctcggtgaaa aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat
    70621 tgccaaccgt atcgtcgaag aagcgatgat tgccgctaac atttgtgcgg cccgcgtact
    70681 gcgcgataag ctcggttttg gcatctataa cgtgcatatg ggctttgatc cggcgaatgc
    70741 cgacgcgctg gcagcgttgc tgaaaacgca cggtctgcat gtcgatgccg aagaagtgct
    70801 cacgctggac ggtttctgca aactgcgtcg tgaactggac gcgcaaccaa ctggtttcct
    70861 cgacagccgc attcgtcgct tccagtcatt tgctgaaatt agcactgaac ccggtcctca
    70921 ctttggcctc ggtctggaag catacgccac ctggacttcg ccgatccgta aatatggcga
    70981 catgatcaac caccgtctgc tgaaagcggt tatcaaaggc gaaactgcga cgcgcccaca
    71041 ggatgaaatc actgtccaaa tggccgagcg tcgccgtctc aaccggatgg cagaacgtga
    71101 tgttggtgac tggttatacg cacgcttcct gaaagacaaa gccgggaccg acacccgttt
    71161 cgcagcggaa attgtcgata tcagccgtgg cggcatgcgt gttcgtttgg ttgataacgg
    71221 cgctatcgcc tttattccgg cacctttctt acacgctatg cgcgatgaac tggtttgcag
    71281 ccaggaaaac ggcactgtac aaattaaagg tgaaacggtt tacaaagtga ctgacgttat
    71341 tgacgtcact attgccgaag tccgcatgga aacccgcagc attattgcgc gcccggtcgc
    71401 gtaatctcct ttcacggccc attcctcatg gatgggccgt ttatttcccc gctctacctt
    71461 cgttatatcc aagcagattt tttaactgac tttcgtttga aaactggcgt ttttccaaaa
    71521 ctggattaca aataaatata taaaaaaaac aatgcgttta gccgccgtta atttcatgta
    71581 agtcatgttg ttccttttat ttaaccgcgg actccgcagt taaccggagg atatgcatca
    71641 tgaaaaccgt tagggagtcc acaacgttgt acaactttct cggatcgcac aatccatact
    71701 ggcggttgac ggaaagcagc gatgttttgc gcttttctac caccgaaacc acagaacctg
    71761 atcgtacatt gcagttatct gccgaacagg ctgctcgcat cagggaaatg acggtcatca
    71821 cctccagcct gatgatgagt ctgaccgtcg atgaaagcga tctttctgtg catctggtag
    71881 gacgaaaaat caataaacgg gaatgggctg gcaacgcgtc tgcatggcat gacacaccgg
    71941 cggttgctcg tgatttatca cacgggcttt cctttgctga gcaggtagtt tctgaagcac
    72001 attccgcaat agtgattctc gacagccggg ggaatatcca acgcttcaat cggttatgtg
    72061 aagattacac agggttgaaa gaacacgacg tcattgggca aagcgtgttt aaactgttta
    72121 tgagccgtcg tgaagctgcg gcatccaggc gcaataaccg tgtatttttt cgaagcggca
    72181 atgcatatga agtcgaactg tggataccaa catgtaaagg ccagcggctg tttctgtttc
    72241 gcaataaatt tgtccacagc ggcagtggca aaaacgagat ttttttaatc tgttccggca
    72301 ccgacattac cgaagagcgc cgcgctcagg agcgactgcg tattctggca aataccgaca
    72361 gtatcaccgg actgccgaat cgtaacgcaa tgcaggattt aatcgatcac gctattaatc
    72421 atgcagataa caataaagtt ggggttgtgt atcttgattt ggataatttc aaaaaggtca
    72481 acgacgccta tgggcatttg tttggtgacc agttattacg cgacgtgtca ttggctattt
    72541 taagctgtct cgaacatgac caggtgttgg cgcgtccagg tggggatgag tttctggtac
    72601 tggcatccaa cacctcacaa agcgcgctgg aagcaatggc atcacgaatt ttgacccgct
    72661 tacggctccc ctttcgcatt ggtttaattg aagtttatac cagctgttca gtaggtattg
    72721 cactctctcc cgaacatggt tcagacagca cggctattat tcgtcacgcc gacacagcaa
    72781 tgtacacagc gaaggaaggc ggacgaggac aattttgcgt ttttacccca gaaatgaatc
    72841 aacgggtatt tgaatatctc tggctggata ccaacttgcg taaagcactg gaaaacgatc
    72901 agttggttat tcactatcaa ccgaaaatca cctggcgtgg cgaagtgcgc agtctggaag
    72961 cactagtacg ttggcagtca cctgaacgtg ggttgattcc accgttggac ttcatttcct
    73021 acgccgaaga gtcagggcta attgtgcctt taggccgttg ggtgattctc gatgtcgtac
    73081 gccaggtggc aaagtggcgg gaaaaaggca taaacctgcg agtggcggta aatatttctg
    73141 cacgtcagct cgccgatcaa accattttca ccgccctgaa acaggttctc caggaactca
    73201 attttgaata ctgccctata gatgttgaac tgacagagag ttgtctgatt gagaatgatg
    73261 aactggcact gtctgttatt caacaattta gccaactagg tgcgcaagtg catctggatg
    73321 attttggtac cggctactct tcactttcgc aactggcgcg ctttccgatc gatgccatca
    73381 aacttgacca ggtttttgtt cgagatattc acaaacaacc tgtctcgcag tcactggtcc
    73441 gggcgatcgt cgctgtggcc caggcattga atcttcaggt gatcgccgaa ggtgtagaga
    73501 gtgcaaagga agatgctttt ttaaccaaga acgggatcaa tgagcggcaa ggatttttgt
    73561 ttgccaaacc gatgcccgcc gtcgccttcg aacgctggta taaacgctat ctgaagcgcg
    73621 cataagtaga tgaatatata tacttagcgc tatatcccgt cattatatat cgtattcctg
    73681 gatgataatt agccgttgct gttaaacact tctcttttag cggcaatttt tgcattattc
    73741 attttccccc ttttgctata aggatgtcct tgatgtctga atttgatgcc cagcgcgttg
    73801 ccgaacgtat tgatattgtg ttagatatcc ttgtggcagg cgattaccac tctgcgatcc
    73861 ataatctcga gatacttaaa gcagaactct tgcgccaggt tgctgaatct acgccagaca
    73921 tcccgaaggc accgtgggag atttaacaat cgtgccttca ggttttccta tttgaggatg
    73981 cccggcgcgt cagtattttt ttatttagta ttataacgtt ataagaatta cagcgatgaa
    74041 ttcccgacaa caaactattc tacagatggt cattgaccag ggtcaggtta gcgtaaccga
    74101 tctggcaaaa gccactggag tttctgaagt taccattcgc caggatctca acaccctcga
    74161 aaaactgagt tacctccgcc gtgcacatgg ctttgcagtt tcgcttgata gtgatgacgt
    74221 cgaaacccgt atgatgagca actatacgct gaagcgtgaa ctcgccgagt ttgccgcgtc
    74281 tctggttcaa ccgggcgaaa ccatctttat cgaaaatggt agcagtaatg ccctgctcgc
    74341 ccggactctg ggcgaacaga agaaaaatgt cactatcatc acagtcagca gctacattgc
    74401 gcatttgcta aaagacgcgc cttgtgaagt cattttgctc ggtggcgtgt accagaaaaa
    74461 aagcgaaagt atggttggcc ctttgacacg ccagtgcatc cagcaggtac atttcagcaa
    74521 agcatttatt ggtattgatg gctggcaacc tgaaactgga ttcaccggtc gcgatatgat
    74581 gcgtaccgat gtggtcaatg ccgtgctgga aaaagagtgc gaagcgatag tcctgactga
    74641 cagctcgaaa tttggtgctg tacattcata ctccatcggt cccgttgagc gattcaatcg
    74701 cgtgattacc gattcgaaaa tacgcgccag cgatctgatg catcttgagc acagtaaact
    74761 caccgttcac gtcgttgaca tttaaccatt tcccttctct acggatgatt tgcagtttgg
    74821 caaatcatcc gctctaagat gattcctggt tgataattaa gactatttac ctgttattaa
    74881 cacgcttaag atataaaatt attatcagcg atataacagg aagtcattat cacctgcgtg
    74941 atataaccct gcgcgcgagc agatttcacg gaataatttc accaggctta ttcttagcta
    75001 ttatagttat agagagctta cttccgtgaa tcataaattc aggagagagt attatgtttg
    75061 taacgagcaa aaaaatgacc gcggctgttc tggcaattac tttggcaatg tctctgagtg
    75121 cctgttctaa ctggtctaaa cgggaccgca acaccgcaat cggcgcgggt gcaggggcat
    75181 taggcggtgc agtactgacc gatggcagta cgttgggtac attaggtggt gcagctgtcg
    75241 gtggtgttat tggtcaccag gtcggtaaat aatcgtaaat aaatgtcgat tatcgactcg
    75301 caattataat acgcttgatt ctgttcagta cgaaataact gaataataca cacagtaaaa
    75361 tattcgaaag ccacggatat atccgtggct ttttatgtta accgcctgcg agttttacct
    75421 tcatcccttt cgcttcaagt agtgatttta ataaatcacg cttatcgccc tggatttcaa
    75481 taactccatc tttaactgct ccgccgcagc cgcatttttt cttcagttcc gctgcaagtt
    75541 ttgtcagttc ggcatcatcg agatcgacac cggtaatcag gcaaacgccc ttacctttac
    75601 gtccactggt ctgacgctga atacgcacca caccgtcgcc tttagggcgt acaggggccg
    75661 ctttgggctc atcaatacgt ccggtttccg ttgagtagac cagacggctg ttggaatcac
    75721 tcatcatgca ctccgctgta aagaggcgtt gatcgctttc agcgtctgcg ctggatctac
    75781 cgattgcgtt accgggcgac caatcaccat ataatcaaca ccagccgaca acgcctgttc
    75841 tggcgtcata atgcggcgct ggtcaccagc ttcactcccc tgcggacgaa tgcccggcgt
    75901 aaccagtttg aactcctgac cgaatacctg tttaaagcgc acagcttcct gagcagaaca
    75961 caccacacca tcaaggccac atttttgcgt cagtgccgcc agacgttctg cataatctgc
    76021 aggtgacagt gtcatgccaa gatcgaccag gtcgctggct tccatgctgg tcaacactgt
    76081 cacagcaatc aaaagcggtg catctttgcc aaacggaacc agtgcctcac gcgctgcggt
    76141 catcatacgc gccccaccag aggcatgaac attcaccatc cacacgccta agtcagctgc
    76201 agcagcgaca gcgtgcgcag cagtgttagg aatatcgtgg aatttcaggt caagaaagat
    76261 atcaaaacca cgctgttgaa gttcgcgcac aaactgtggc ccaaacaatg taaacatctc
    76321 tttgccgacc ttcagacgac aatcgcgtgg gtcgatcttg tcgacaaagg acagcgcgtc
    76381 atcacgatta tgataatcaa gggcaacaac cacaggagaa ttcgtaacag cgcgggaaga
    76441 agatgaagca gttaacgtca tgaccagacc ttcttgatga tgggcaccgg aagtggcgcg
    76501 aaacaggtaa acggcgagca ttctacccgt ggaaataaaa aattaacagt tgcgattttt
    76561 cccctggcag gctttttctg cgccgtcgtt gccggagcac aatggaacat caataactag
    76621 tatgttgtaa ctaaagtagg attttttaaa aattacaggc catcaagacc gcgaatcggt
    76681 ttaatggttg accaggcccg acaagacgga caatgccagt agagggtgta tgcggtaaaa
    76741 ccacatttct ggcagcgata acgaggctta ctacgtacct tttcgccaac catgtcacgc
    76801 agcaccatca ggctctcttt ggcacgccct tcttccgctt catttaagtg gtaatccatt
    76861 aacttatgga acacacgcat ggtcggatga cgctgaagct ggcgcgtaat atagacctgt
    76921 gcggcctcac taccgtcgcg cgcttcgatg atatcagcaa gcatcaattc agcatcggca
    76981 ccggtgttct cttccaccgc gcgttgcagg aattctgccc attcggcagt tttacccaac
    77041 tgctgatagc aggtttgcaa catttccagc gtttcgctga ccagttctct gtcctgggat
    77101 atcacgcgtt gcaaactttc gacggctttg gcgtattctc ctttcgccat aaacacgcgt
    77161 cccatcatga tggatacgcg ggcgctgttt ttatctgccg ccgccccttt ttttagcaag
    77221 gtcatggcac gatcgagatc gtcgctggcc atatgctgca gggctaactc acagtagaaa
    77281 tgggcaattt cgacgcgctg tttatcttta cccagcttca ccaggcgttc ggcaacatca
    77341 attgctttct gccactcact ggtagcctgg tagatttgta gcaactgttg cagagcgcca
    77401 atgcggaagt cagtttcatc ggtcagctga ttgaacatgt cttccgcgcg gtcgtataac
    77461 ccggcggcca tgtaatcacg ccctagttgt tgaatcgcca acagacgctg ttcataggtc
    77521 agcgaggcgc tttccattag ggtctgatgg atgcgaatag cgcgatcaac ttcgccacgc
    77581 gaacggaaca ggtttccgag cgtaaggtgg gcttcaacgg tgcctgtatc ctctttaagc
    77641 atatcgagaa acaggtctac cgctttatcc tgttgattac taagcaggaa gttaaccccc
    77701 gctacgtaat cacgcgacaa gcggttggct tcatcttgct tgttttgttg cgcacttctg
    77761 cggcccatat accagccata ggcagcggct acaggcaaaa gcagaaacaa caactccagc
    77821 atagaaagtt attccttcgc cgctgacgag tgcggcacta cagccacgtc agtcgcgggt
    77881 gaaagctggt tttccagtcg ctttatttta cgttcagcgc gcgccaggga aacacgaact
    77941 cgcagccaga acaggccaca aatcaaccaa ccgatagcaa accccgcagc aaacaatacc
    78001 gccagcaatg tggaaatacg gtactcccct tgcgctaaca gataattaaa cgtcacctgt
    78061 tgatcgttct gcgcacccaa cgtgaccgaa atcacgaaga tcgctaacac cagtaagaaa
    78121 atgagtaaat atttcacatt acttcccgtt atgtggttag agcgaataaa ttatggtcaa
    78181 cttaccgcaa tagcccttta aagtaccatt ttcgcgccgt ctgcgaaatg gcatccacgc
    78241 gcattgttga tgatttaagg gctaaattcg gaaaatcaac cagcgttaac tttcttgttc
    78301 tcgttgcgct atttcgcgat tttcttccgc aggtggtgtt aatggcccac aaattcgttg
    78361 cgcaagccac gttgccaccg ccaccagcgc ccacgaaatc aacgtagcta ctaccagatc
    78421 gcgtggccaa tgcatcccga gcagcaggcg gcttcccatg actcccgttg cccagaccag
    78481 caagatagca atggttaacg ttcgccgacg cggccacagc aaaccaacgg ccagcagtgc
    78541 ccaactggca gcaaacatcg tgtgaccgga aggaaaggca aaccccgtct ctttctgcca
    78601 atgtgaacgc aaatattgtg ggatattttt ctcttcagcc aactgttctt tcactagatt
    78661 tccgcgttct gctcgcttta aagtgtagaa ctcatcaacc ggaatatgat gtgttttttc
    78721 cagccagata acaaaaggtc gtggttcctg gactttgtct ttgatccagg atttaacgcc
    78781 ttgtcccaca aggattgcgg ccgccagaat ggcaaataat acaaaggcag ccttaatgcg
    78841 aaaacgcaga caccagagaa accagccgaa taaaatcaaa tgtgtaatga cgccccaggg
    78901 ctgggtgaca gtttcagtaa cccaaaaagc cgcttttagt agccaacttt gttctccagg
    78961 ttgccaacgc cagccagaaa tccatacggc tactggcatg acaagcaata gtgcagctcc
    79021 cactgcggta cgtctggcaa tcgaacgcat ggcctctcct ttttgataag tcccacaatc
    79081 ataactgaaa acgccagttc caggaaaaat tgacagattt gtgccattcc gtgaacgatc
    79141 gacgcgtcgt gattaggtga accccttctc gttatggcaa aataagccaa tacagaacca
    79201 gcattatctg gagaatttca tgcagcttaa acgtgtggca gaagccaaac tgccaacccc
    79261 atggggcgat ttcctgatgg tgggatttga agaactggca accggacacg atcatgtcgc
    79321 gctagtctat ggcgatattt ccgggcatac cccggtactt gcgcgcgtcc attccgaatg
    79381 tctgaccggt gacgccctgt tcagcttgcg ctgcgattgt ggcttccagc tcgaagcggc
    79441 attgacgcaa attgccgagg aaggccgtgg tattttgctg tatcaccgtc aggaaggtcg
    79501 taacattggt ctgctgaata aaatccgcgc ttacgcactg caggatcaag gttacgatac
    79561 cgtagaggct aaccaccagt taggcttcgc cgctgatgag cgcgacttca ctctttgcgc
    79621 tgatatgttc aaactccttg gcgtcaatga agtccgcttg ttaaccaata acccgaaaaa
    79681 agtcgaaatt ctgaccgaag cagggattaa tattgttgaa cgcgtaccat tgattgtagg
    79741 tcgtaacccc aataacgaac attatctcga taccaaagcc gagaaaatgg gccatttgct
    79801 gaacaaataa ccctcttgca ttgtgtaatt aatttgcttg ccggaagcaa aataaccggc
    79861 aagcaaatac ttgttacttc aacatattac gaatgacata atgcaaaatg ccgtcgttct
    79921 ggtagtaggt caactccgtc gcggtgtcga tacgacaacg gcagggtacg acctcctggc
    79981 taccatccgc gcgcgtaagc gtcaccggaa ccgtcgcacc gggttgtaga ttttgcagat
    80041 cgccaatatc aatcttctct tccccggtta gtcctaacgt tttacgcgtt acgccttgcg
    80101 gaaattccag cggcaggatg cccatgccaa ttaaattcga acggtgaatt cgttcaaacg
    80161 attcggcaat caccacacga attccaagca gacgaggacc ttttgccgcc cagtcacggc
    80221 ttgagcctga tccatactct ttcccggcaa tcaccgccag cggcgtttgt tccttcttat
    80281 agcgcatcgc agcatcataa atagagacta cgtcgctgtc aggtaaatgc cgcgtcatcc
    80341 ccccttcaac gccaggcacc atttcattac ggatgcgaat attggcgaag gtgccgcgca
    80401 tcatcacttc atggttacca cgccgcgaac cgtaggagtt aaagtctttt cgctcaacac
    80461 cccgaccttg tagatatcga cccgctgggc tgtcgggctt aatactgccc gccggagaga
    80521 tatggtcagt ggtgactgaa tcccccagca ttgcgaggat ccgcgcaccg tgaatatctt
    80581 ccactggtgc tggtgttgcc tgcatttcat caaagaaagg cgataagcga atataggttg
    80641 agtcctcctg ccaaccgtag gtatcggatc gtgtgacgtt aatttccttc cactctgctg
    80701 tgccttcaaa aacttctgcg tactctttgc ggaacatttc tgtggagact tgttctaccg
    80761 cacgggcaat ttcttgtgcc gatggccaga tatctttcag ataaaccggg tcgcctttac
    80821 gatcatgacc aataggctca gcagccaggt tgatattcat atttcccgcc agcgcatagg
    80881 caaccaccag cggcggcgag gccagccagt tagttttaac cagcggatgg atacggcctt
    80941 caaagttacg gttgccggac agcaccgcac cgacggttaa atcgcctttt ttgattgccg
    81001 tttcgatagg atcgggcagc ggcccagagt taccaataca ggtggtacaa ccgtatccca
    81061 caaggttaaa ccccagttcg tcgagatacg gtgtcagttt cgcttttgcc agataatcag
    81121 aaacgacttt cgaacccggt gccagcgacg ctttgaccca cggttgccgc ttgaggccca
    81181 gagttacggc ttttttcgcc agcaagcctg cggccatcag cacacttggg ttagaggtgt
    81241 tggtgcacga ggttatcgca gcaatgacca cagcgccatc aggtaactga tactgatgtc
    81301 cgttcataac ataatcgacc ggctggcgat ctttatgcgt ggcattcact tccagttcgt
    81361 tactggcggc aaatgctttt ggtacatcgg gcagtgcaac gcgatcctgt gggcgttttg
    81421 gccctgccag gctcgcttca acgtcattca tatccagttc taacgtactg gtaaaaatcg
    81481 gttcatcgcc cgggttacgc cacatgccct gcgctttggc atatttttcg accaactcaa
    81541 cctgatcttc gctgcgcccg cttaaacgca tgtaatcgag ggttacagca tcgattggga
    81601 agaagccaca ggtggcacca tattctggcg acatattggc aatggtggcg cgatccgcca
    81661 gcggtagtga atccagacca tcaccataaa attcgacgaa tttccccacc acgccatgtt
    81721 tgcgcagcat ttgggtaaca gtgagaacca ggtctgtggc ggtaatacct tcacgtaatt
    81781 taccggtaag tttaaagccc actacgtccg ggataagcat ggaaaccgg
//
LOCUS       DAZFJF010000008        73642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFJF010000008 DAZFJF010000000
VERSION     DAZFJF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.008"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(136..212)
                     /locus_tag="ACHKAU_000615"
     tRNA            complement(136..212)
                     /locus_tag="ACHKAU_000615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Arg,seq:acg)
     gene            complement(276..352)
                     /locus_tag="ACHKAU_000616"
     tRNA            complement(276..352)
                     /locus_tag="ACHKAU_000616"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Arg,seq:acg)
     gene            complement(550..626)
                     /locus_tag="ACHKAU_000617"
     tRNA            complement(550..626)
                     /locus_tag="ACHKAU_000617"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(590..592),aa:Arg,seq:acg)
     gene            complement(630..722)
                     /locus_tag="ACHKAU_000618"
     tRNA            complement(630..722)
                     /locus_tag="ACHKAU_000618"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(686..688),aa:Ser,seq:gct)
     gene            complement(1038..1223)
                     /gene="csrA"
                     /locus_tag="ACHKAU_000619"
     CDS             complement(1038..1223)
                     /gene="csrA"
                     /locus_tag="ACHKAU_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO5529743.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1458..4088)
                     /gene="alaS"
                     /locus_tag="ACHKAU_000620"
     CDS             complement(1458..4088)
                     /gene="alaS"
                     /locus_tag="ACHKAU_000620"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO5529744.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4216..4716)
                     /gene="recX"
                     /locus_tag="ACHKAU_000621"
     CDS             complement(4216..4716)
                     /gene="recX"
                     /locus_tag="ACHKAU_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO5529745.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4959..6020)
                     /gene="recA"
                     /locus_tag="ACHKAU_000622"
     CDS             complement(4959..6020)
                     /gene="recA"
                     /locus_tag="ACHKAU_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO5529746.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6100..6597)
                     /gene="pncC"
                     /locus_tag="ACHKAU_000623"
     CDS             complement(6100..6597)
                     /gene="pncC"
                     /locus_tag="ACHKAU_000623"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO5529747.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6742..7827)
                     /gene="mltB"
                     /locus_tag="ACHKAU_000624"
     CDS             complement(6742..7827)
                     /gene="mltB"
                     /locus_tag="ACHKAU_000624"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO5529748.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8083..8646
                     /gene="srlA"
                     /locus_tag="ACHKAU_000625"
                     /gene_synonym="gutA"
     CDS             8083..8646
                     /gene="srlA"
                     /locus_tag="ACHKAU_000625"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO5529749.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8643..9602
                     /gene="srlE"
                     /locus_tag="ACHKAU_000626"
                     /gene_synonym="gutE"
     CDS             8643..9602
                     /gene="srlE"
                     /locus_tag="ACHKAU_000626"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO5529750.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9613..9984
                     /gene="srlB"
                     /locus_tag="ACHKAU_000627"
                     /gene_synonym="gutB"
     CDS             9613..9984
                     /gene="srlB"
                     /locus_tag="ACHKAU_000627"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO5529751.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9988..10767
                     /gene="srlD"
                     /locus_tag="ACHKAU_000628"
     CDS             9988..10767
                     /gene="srlD"
                     /locus_tag="ACHKAU_000628"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO5529752.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10872..11231
                     /gene="gutM"
                     /locus_tag="ACHKAU_000629"
     CDS             10872..11231
                     /gene="gutM"
                     /locus_tag="ACHKAU_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO5529753.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11298..12071
                     /gene="srlR"
                     /locus_tag="ACHKAU_000630"
                     /gene_synonym="gutR"
     CDS             11298..12071
                     /gene="srlR"
                     /locus_tag="ACHKAU_000630"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO5529754.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            12064..13029
                     /gene="gutQ"
                     /locus_tag="ACHKAU_000631"
     CDS             12064..13029
                     /gene="gutQ"
                     /locus_tag="ACHKAU_000631"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO5529755.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(13026..14540)
                     /gene="norR"
                     /locus_tag="ACHKAU_000632"
     CDS             complement(13026..14540)
                     /gene="norR"
                     /locus_tag="ACHKAU_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO5529756.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14727..16166
                     /gene="norV"
                     /locus_tag="ACHKAU_000633"
     CDS             14727..16166
                     /gene="norV"
                     /locus_tag="ACHKAU_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO5529757.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            16163..17296
                     /gene="norW"
                     /locus_tag="ACHKAU_000634"
     CDS             16163..17296
                     /gene="norW"
                     /locus_tag="ACHKAU_000634"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO5529758.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17522..19774)
                     /gene="hypF"
                     /locus_tag="ACHKAU_000635"
     CDS             complement(17522..19774)
                     /gene="hypF"
                     /locus_tag="ACHKAU_000635"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO5529759.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19927..20454)
                     /gene="hydN"
                     /locus_tag="ACHKAU_000636"
     CDS             complement(19927..20454)
                     /gene="hydN"
                     /locus_tag="ACHKAU_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO5529760.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20603..21613)
                     /gene="ascG"
                     /locus_tag="ACHKAU_000637"
     CDS             complement(20603..21613)
                     /gene="ascG"
                     /locus_tag="ACHKAU_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO5529761.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            21873..23330
                     /gene="ascF"
                     /locus_tag="ACHKAU_000638"
     CDS             21873..23330
                     /gene="ascF"
                     /locus_tag="ACHKAU_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO5529762.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            23339..24763
                     /gene="ascB"
                     /locus_tag="ACHKAU_000639"
     CDS             23339..24763
                     /gene="ascB"
                     /locus_tag="ACHKAU_000639"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO5529763.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24877..25296)
                     /locus_tag="ACHKAU_000640"
     CDS             complement(24877..25296)
                     /locus_tag="ACHKAU_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5529764.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(25293..25604)
                     /locus_tag="ACHKAU_000641"
     CDS             complement(25293..25604)
                     /locus_tag="ACHKAU_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO5529765.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25778..26521)
                     /locus_tag="ACHKAU_000642"
     CDS             complement(25778..26521)
                     /locus_tag="ACHKAU_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529766.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26564..27034)
                     /gene="hycI"
                     /locus_tag="ACHKAU_000643"
     CDS             complement(26564..27034)
                     /gene="hycI"
                     /locus_tag="ACHKAU_000643"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO5529767.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(27027..27437)
                     /gene="hycH"
                     /locus_tag="ACHKAU_000644"
     CDS             complement(27027..27437)
                     /gene="hycH"
                     /locus_tag="ACHKAU_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO5529768.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(27434..28201)
                     /gene="hycG"
                     /locus_tag="ACHKAU_000645"
     CDS             complement(27434..28201)
                     /gene="hycG"
                     /locus_tag="ACHKAU_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO5529769.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(28201..28743)
                     /gene="hycF"
                     /locus_tag="ACHKAU_000646"
     CDS             complement(28201..28743)
                     /gene="hycF"
                     /locus_tag="ACHKAU_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO5529770.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28753..30462)
                     /gene="hycE"
                     /locus_tag="ACHKAU_000647"
     CDS             complement(28753..30462)
                     /gene="hycE"
                     /locus_tag="ACHKAU_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO5529771.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30480..31403)
                     /gene="hycD"
                     /locus_tag="ACHKAU_000648"
     CDS             complement(30480..31403)
                     /gene="hycD"
                     /locus_tag="ACHKAU_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO5529772.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(31406..33232)
                     /gene="hycC"
                     /locus_tag="ACHKAU_000649"
     CDS             complement(31406..33232)
                     /gene="hycC"
                     /locus_tag="ACHKAU_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO5529773.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(33229..33840)
                     /gene="hycB"
                     /locus_tag="ACHKAU_000650"
     CDS             complement(33229..33840)
                     /gene="hycB"
                     /locus_tag="ACHKAU_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO5529774.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33965..34426)
                     /gene="hycA"
                     /locus_tag="ACHKAU_000651"
     CDS             complement(33965..34426)
                     /gene="hycA"
                     /locus_tag="ACHKAU_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO5529775.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34638..34988
                     /gene="hypA"
                     /locus_tag="ACHKAU_000652"
     CDS             34638..34988
                     /gene="hypA"
                     /locus_tag="ACHKAU_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5529776.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34992..35864
                     /gene="hypB"
                     /locus_tag="ACHKAU_000653"
     CDS             34992..35864
                     /gene="hypB"
                     /locus_tag="ACHKAU_000653"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO5529777.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35855..36127
                     /gene="hypC"
                     /locus_tag="ACHKAU_000654"
     CDS             35855..36127
                     /gene="hypC"
                     /locus_tag="ACHKAU_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO5529778.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            36127..37248
                     /gene="hypD"
                     /locus_tag="ACHKAU_000655"
     CDS             36127..37248
                     /gene="hypD"
                     /locus_tag="ACHKAU_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO5529779.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            37245..38255
                     /gene="hypE"
                     /locus_tag="ACHKAU_000656"
     CDS             37245..38255
                     /gene="hypE"
                     /locus_tag="ACHKAU_000656"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO5529780.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            38329..40407
                     /gene="flhA"
                     /locus_tag="ACHKAU_000657"
     CDS             38329..40407
                     /gene="flhA"
                     /locus_tag="ACHKAU_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO5529781.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(40444..40797)
                     /gene="ygbA"
                     /locus_tag="ACHKAU_000658"
     CDS             complement(40444..40797)
                     /gene="ygbA"
                     /locus_tag="ACHKAU_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO5529782.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40874..41008)
                     /locus_tag="ACHKAU_000659"
     CDS             complement(40874..41008)
                     /locus_tag="ACHKAU_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529783.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            41084..43645
                     /gene="mutS"
                     /locus_tag="ACHKAU_000660"
     CDS             41084..43645
                     /gene="mutS"
                     /locus_tag="ACHKAU_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO5529784.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43751..44407
                     /gene="pphB"
                     /locus_tag="ACHKAU_000661"
     CDS             43751..44407
                     /gene="pphB"
                     /locus_tag="ACHKAU_000661"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5529785.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(44458..45225)
                     /gene="ygbI"
                     /locus_tag="ACHKAU_000662"
     CDS             complement(44458..45225)
                     /gene="ygbI"
                     /locus_tag="ACHKAU_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO5529786.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            45421..46329
                     /gene="ltnD"
                     /locus_tag="ACHKAU_000663"
     CDS             45421..46329
                     /gene="ltnD"
                     /locus_tag="ACHKAU_000663"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO5529787.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            46326..47588
                     /gene="otnK"
                     /locus_tag="ACHKAU_000664"
     CDS             46326..47588
                     /gene="otnK"
                     /locus_tag="ACHKAU_000664"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO5529788.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            47585..48223
                     /gene="ygbL"
                     /locus_tag="ACHKAU_000665"
     CDS             47585..48223
                     /gene="ygbL"
                     /locus_tag="ACHKAU_000665"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO5529789.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            48228..49004
                     /gene="ygbM"
                     /locus_tag="ACHKAU_000666"
     CDS             48228..49004
                     /gene="ygbM"
                     /locus_tag="ACHKAU_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO5529790.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            49093..50457
                     /gene="ygbN"
                     /locus_tag="ACHKAU_000667"
     CDS             49093..50457
                     /gene="ygbN"
                     /locus_tag="ACHKAU_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO5529791.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(50496..50732)
                     /locus_tag="ACHKAU_000668"
     CDS             complement(50496..50732)
                     /locus_tag="ACHKAU_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO5529792.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(50743..52170)
                     /locus_tag="ACHKAU_000669"
     CDS             complement(50743..52170)
                     /locus_tag="ACHKAU_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO5529793.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(52170..52763)
                     /locus_tag="ACHKAU_000670"
     CDS             complement(52170..52763)
                     /locus_tag="ACHKAU_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO5529794.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            52910..53317
                     /locus_tag="ACHKAU_000671"
     CDS             52910..53317
                     /locus_tag="ACHKAU_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO5529795.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(53438..54430)
                     /gene="rpoS"
                     /locus_tag="ACHKAU_000672"
     CDS             complement(53438..54430)
                     /gene="rpoS"
                     /locus_tag="ACHKAU_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO5529796.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(54493..55587)
                     /gene="nlpD"
                     /locus_tag="ACHKAU_000673"
     CDS             complement(54493..55587)
                     /gene="nlpD"
                     /locus_tag="ACHKAU_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO5529797.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(55772..56398)
                     /gene="pcm"
                     /locus_tag="ACHKAU_000674"
     CDS             complement(55772..56398)
                     /gene="pcm"
                     /locus_tag="ACHKAU_000674"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO5529798.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(56392..57153)
                     /gene="surE"
                     /locus_tag="ACHKAU_000675"
     CDS             complement(56392..57153)
                     /gene="surE"
                     /locus_tag="ACHKAU_000675"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO5529799.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(57134..58183)
                     /gene="truD"
                     /locus_tag="ACHKAU_000676"
     CDS             complement(57134..58183)
                     /gene="truD"
                     /locus_tag="ACHKAU_000676"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO5529800.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(58180..58659)
                     /gene="ispF"
                     /locus_tag="ACHKAU_000677"
     CDS             complement(58180..58659)
                     /gene="ispF"
                     /locus_tag="ACHKAU_000677"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO5529801.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(58659..59369)
                     /gene="ispD"
                     /locus_tag="ACHKAU_000678"
     CDS             complement(58659..59369)
                     /gene="ispD"
                     /locus_tag="ACHKAU_000678"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO5529802.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(59388..59699)
                     /gene="ftsB"
                     /locus_tag="ACHKAU_000679"
     CDS             complement(59388..59699)
                     /gene="ftsB"
                     /locus_tag="ACHKAU_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO5529803.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(59893..60216)
                     /gene="ygbE"
                     /locus_tag="ACHKAU_000680"
     CDS             complement(59893..60216)
                     /gene="ygbE"
                     /locus_tag="ACHKAU_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO5529804.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(60266..60871)
                     /gene="cysC"
                     /locus_tag="ACHKAU_000681"
     CDS             complement(60266..60871)
                     /gene="cysC"
                     /locus_tag="ACHKAU_000681"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO5529805.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(60871..62298)
                     /gene="cysN"
                     /locus_tag="ACHKAU_000682"
     CDS             complement(60871..62298)
                     /gene="cysN"
                     /locus_tag="ACHKAU_000682"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO5529806.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(62300..63208)
                     /gene="cysD"
                     /locus_tag="ACHKAU_000683"
     CDS             complement(62300..63208)
                     /gene="cysD"
                     /locus_tag="ACHKAU_000683"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO5529807.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            63460..64497
                     /gene="iap"
                     /locus_tag="ACHKAU_000684"
     CDS             63460..64497
                     /gene="iap"
                     /locus_tag="ACHKAU_000684"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO5529808.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   64579..65034
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=64640..64668
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(65131..65424)
                     /gene="cas2e"
                     /locus_tag="ACHKAU_000685"
     CDS             complement(65131..65424)
                     /gene="cas2e"
                     /locus_tag="ACHKAU_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO5529809.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(65424..66344)
                     /gene="cas1e"
                     /locus_tag="ACHKAU_000686"
     CDS             complement(65424..66344)
                     /gene="cas1e"
                     /locus_tag="ACHKAU_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO5529810.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(66341..66991)
                     /gene="cas6e"
                     /locus_tag="ACHKAU_000687"
     CDS             complement(66341..66991)
                     /gene="cas6e"
                     /locus_tag="ACHKAU_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO5529811.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(66973..67719)
                     /gene="cas5e"
                     /locus_tag="ACHKAU_000688"
     CDS             complement(66973..67719)
                     /gene="cas5e"
                     /locus_tag="ACHKAU_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO5529812.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(67730..68785)
                     /gene="cas7e"
                     /locus_tag="ACHKAU_000689"
     CDS             complement(67730..68785)
                     /gene="cas7e"
                     /locus_tag="ACHKAU_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO5529813.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(68797..69336)
                     /gene="casB"
                     /locus_tag="ACHKAU_000690"
                     /gene_synonym="cse2"
     CDS             complement(68797..69336)
                     /gene="casB"
                     /locus_tag="ACHKAU_000690"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO5529814.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            complement(69333..70895)
                     /gene="casA"
                     /locus_tag="ACHKAU_000691"
                     /gene_synonym="cse1"
     CDS             complement(69333..70895)
                     /gene="casA"
                     /locus_tag="ACHKAU_000691"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO5529815.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(70993..73642)
                     /gene="cas3"
                     /locus_tag="ACHKAU_000692"
                     /pseudo
     CDS             complement(70993..73642)
                     /gene="cas3"
                     /locus_tag="ACHKAU_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT        17338 a        20160 c        18895 g        17249 t
ORIGIN      
        1 tggtgcatcc gggaggatta ctcgccttgc ggctcgccct tcgggccgtt gctaaagcaa
       61 cgttatcatc cctggtgcct gcggttaatt ccgctacctc ttcgatacct tcattgctga
      121 agatttcgga gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag
      181 ccgagtactc tatccagctg agctacggat gcatcgggaa aatttatttt actgctgata
      241 ttgataccgc tactaaagcc atatcaagta agagatggtg catccgggag gattcgaacc
      301 tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat gcatcgggaa
      361 acttacttta ctgcagattt tttgataccg ctactaaagc cgtatcaagt aagagatggt
      421 gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgcta aagcaacgtt
      481 atcctccctg gtgcctgcgg ttatacagcc acctcttcca taccttcatc gctgaaagta
      541 cgtagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt
      601 actctatcca gctgagctac ggatgcaaat ggcggtgagg cggggattcg aaccccggat
      661 gcagcttttg accgcatact cccttagcag gggagcgcct tcagcctctc ggccacctca
      721 ccacacgcct cttacgagtg cttcgaagaa cttgtttatc gctcatcgtc gctgcgtggc
      781 gcacatatta ctttctgaga cttataagtc aaacaatttt tcctacactt ttatcgtttg
      841 cacacttcac gttcaattag tctgtaaaaa cgtcaaaaag agtgttttat caacagaaaa
      901 atggaggtct gacagatagt agtaatgcaa aaaaatggag acttaagttg aatgaacggg
      961 agtaaagcga aaagactata gagtgaagga gaaattttga gggtgcgtct caccgataaa
     1021 gatgagacgc ggaaagatta gtaactggac tgctgggatt tttcagcctg gatacgctgg
     1081 tagatctctt cacggtgaac agaaacttcc ttcggggcat ttacgccaat acgtacctgg
     1141 ttgcccttta cccctaaaac tgtcacggtg acctcatccc caatcatgag ggtctcacca
     1201 actcgacgag tcagaatcag cattctttgc tccttgaaag attaaaagag tcgggtctct
     1261 ctgtatcccg gcattatcca tcatataacg ccaaaaagta agcaatgaca aacacattac
     1321 atgtaagcag tcatggcatt acattctgtt aaacctaagt ttagccgata tacacaactt
     1381 caacctgact ttatcgttgt cgatagcgtt aatgcgaatg ccgtgaagcg agtccacggc
     1441 attgcctgac gcttatatta ttgcaatttc gcgctgaccc agcctttcac actggctaac
     1501 gctgcaggta aggccgcagc atccgtacca ccggcttgcg ccatgtcagg acgtccacca
     1561 cccttgccgc ccacctgctg agcgaccata ccaatcagtt cccctgcttt cacacgatct
     1621 gtgacgtcct tagatacgcc tgcaatcaga gaaaccttac cttcggctac cgttgccagc
     1681 acgataattg tcgaccccag ctgatttttt aaatcgtcaa ccatggtacg caacattttc
     1741 ggctcaacac cgctaagctc gctaaccaac agcttaacac cattaacatc aattgcctta
     1801 ctggaaagat ttgcgctctc ctgtgcggca gcttgttctt taagctgttg tagttctttt
     1861 tctaactgac gcgtacgttc cagtactgag cgcactttat ccgccagatt attgctatcg
     1921 cctttcagca gatgcgcgac ttcgcttaag cgatcgctgt ctgcatgaac ggtggtgata
     1981 gcgccttctc cggttaccgc ttcgatacga cgaacgcctg cagcagtacc cgattcagag
     2041 atgatgcgga acagaccaat atcaccagtg cggctggcgt gagtaccgcc acacagctcg
     2101 gtggagaaat cgcccatgct cagcacgcgt acacgctcat catacttctc gccgaacagc
     2161 gccatcgcac ctttcgcttt cgccgcttcg agatccatga tgttggtttc gatcggcaaa
     2221 ttgcgacgaa tctgtgcgtt caccaggtct tcgaccgcac gaatctcttc cggtttcatc
     2281 gcttcgttgt gtgagaagtc gaagcgcagc accttgtcgt taaccagtga acctttctgc
     2341 gatacatgag tccccagaac ctggcgcagc gcagcgtgca tcaggtgcgt tgcggagtga
     2401 ttcagacgaa tacgggcgcg acgagcctca tcaacatcag cctgcacagc gtcgcccact
     2461 ttcagagaac ccgcagcaag tttaccgatg tgaccaatcg cctggccgta tttctgcgta
     2521 tcttccaccg cgaaggagaa gttagcgcct ttcagttcgc ctttatcacc aacctgaccg
     2581 ccggattccg catagaatgg tgtttgatcc agcacgacca cagcttcctg gcctgcattg
     2641 attgcatcaa ccgctttacc atcaacaaac agcgcggtca ctttgccgtt cagttccaga
     2701 tggtcatagc ctttaaattc agatgcacta tcaacacgga tcattgcgtt gtaatcggca
     2761 ccaaagccgc tggcttcgcg cgcgcgacga cgctgctctt ccattgctgc ttcaaaacca
     2821 gcttcgtcaa ctttgatgtt gcgctcacga caaacatcag ccgtcaggtc aaccgggaag
     2881 ccataggtgt cgtacagacg gaaagccgtt tcaccatcca gcgtatcacc agaaagtttt
     2941 gccagctctt catccagcaa cgccagaccg cgctccagag tacgggcaaa ctgctcttct
     3001 tcagtcttca gcacctgctc aacctgcgcc tgctggcgtt tcaggtcttc acccgcagag
     3061 cccataacgt cgatcagcgg accaaccagt ttgtagaaga aggtttcttt cgcgccgagc
     3121 atattgccgt gacgcactgc gcgacgaatg atacgacgca gtacataacc acggttttca
     3181 ttggacggca ttacgccatc cgcgatcagg aacgcacaag aacgaatgtg gtcagcgatt
     3241 acgcgcagcg atttattgct cagatcggtc gcgccagtga ctttcgctac cgcctggatc
     3301 aacgtgcgga acaggtcgat gtcatagtta gagttaacgt gttgcagcac cgcagcaata
     3361 cgctccagac ccataccggt atctacagac ggcttcggca gcggttccat cgtgccatct
     3421 gcctggcggt tgaactgcat gaagacgatg ttccagatct caatgtagcg gtcgccgtct
     3481 tcttccggac ttcccggagg accaccccaa atgtggtcgc cgtgatcgta gaagatttcg
     3541 gtgcacgggc cgcacggacc agtgtcaccc atctgccaga agttgtcaga tgcgtatggc
     3601 gcacctttgt tatcgccgat gcgaataata cgttcgcgcg ggatcccgac ttctttttcc
     3661 cagatttcgt aggcttcgtc gtcgctttca tagacggtaa cccacagacg ctctttcggc
     3721 agggcaaacc atttttcgct ggtcagcagt tcccatgcaa actgaatggc atcgtgtttg
     3781 aaatagtcgc cgaagctgaa gttgcccagc atttcgaaga aggtatggtg acgcgcggtg
     3841 taaccgacgt tttccaggtc gttgtgttta ccacccgcac gcacgcagcg ttgggaagtg
     3901 gtagcgcggg aataattacg cttgtcgagc ccaaggaaca catccttgaa ctggttcatc
     3961 ccggcgttgg taaacaacaa agttgggtcg ttatggggta ccagggagct gctggcaact
     4021 acctgatgtc ccttactatg gaaaaagtcg agaaacgcct gacggatctc agcggtgctc
     4081 ttgctcataa ttatcctgaa atcaagctaa cgaaatatcg ccaccagctc cagcgtgtct
     4141 taaccgccgg gctggtaact gaaaagtggg aataagataa gttttcttga ctgggaagta
     4201 aaataccgta tgcgttcagt cggcaaaatt tcgccaaata tcctggatat cttccatcag
     4261 atagccacga tagagcagaa aacgctggat cttaactttt tctgaaaaga cagttggcaa
     4321 aggttcacca tattttcgcg tcgcctgatc gcgcgccagt gcgcaccagt cgatgtcaca
     4381 ttcacgcatc gctttttctg tcgcttcgcg ggaaatacct ttctgattca gttcctgacg
     4441 aatacgtgca ggtccataac ctttgcggct acggctggcg ataaagcgcg caacaaatcg
     4501 gctgtcatcg agatagccat gttcatggca ccaggcaata acgcgctcgt aatcttctgc
     4561 cgtagcatca atctcttctg ggccattttt gcccataatc ggtgccacga gtttacgtcg
     4621 cagttcttgc tcactgtgat cgcgcaccgc cagaatgcgt accgcacgat ccaacaggcg
     4681 agcatatgcc gggcgacggg acgttgattc tgtcatggca tatccttaca acttaaaaaa
     4741 gcaaaagggc cgcagatgcg acccaggttt tgctgataaa cagatttaat agtcaatgag
     4801 tgtgcagaat gttcccgcag gcccaattcg ttagaggatc gaggtgagag taatttttca
     4861 cgccagtgct ggcctgttcc cctcccccta accctctccc caaaggggcg aggggaccgt
     4921 ccgtgcacac attatctatt atcatcaata acgaacgatt aaaaatcttc gttagtttct
     4981 gctacgcctt cgctgtcatc tacagagaaa tccggcgttg agttcgggtt gctcagcagc
     5041 aactcacgta ctttcttctc gatctctttc gcggtttccg ggttatcttt cagccaggca
     5101 gttgcattcg ctttaccctg accgatcttc tcacctttgt agctgtacca cgcgcctgct
     5161 ttctcgatca gcttctcttt tacgcccagg tcaaccagtt cgccgtagaa gttgatacct
     5221 tcgccgtaga ggatctgaaa ttcagcctgt ttaaacggtg cagcgatttt gttcttcacc
     5281 actttcacgc gggtttcgct acccaccacg ttttcgccct ctttcaccgc gccgatacga
     5341 cggatgtcga gacgaacaga ggcgtagaat ttcagcgcgt taccaccggt agtggtttcc
     5401 gggttaccga acatcacacc aattttcata cggatctggt tgatgaagat cagcagcgtg
     5461 ttggactgct tcaggttacc cgccagctta cgcatcgcct ggctcatcat acgtgccgca
     5521 aggcccatgt gagagtcgcc gatttcgcct tcgatttccg ctttcggcgt cagtgccgcc
     5581 acggagtcaa cgacgataac gtctactgcg ccagaacgcg ccagggcgtc acagatttcc
     5641 agtgcctgct cgccggtgtc cggctgggag cacagcaggt tatcgatatc gacgcccagt
     5701 ttacgtgcgt agattgggtc cagcgcgtgt tcagcatcga taaacgcaca ggttttacct
     5761 tcacgctgcg ctgcggcgat cacctgcagc gtcagcgtgg ttttaccgga agattccggt
     5821 ccgtagattt cgacgatacg gcccatcggc agaccacctg ccccaagcgc gatatccagt
     5881 gaaagcgaac cggtagagat ggtttccaca tccatggaac ggtcttcacc caggcgcatg
     5941 atggagcctt taccaaattg tttctcaatc tggcccagtg ctgccgccaa cgctttctgt
     6001 ttgttttcgt cgatagccat ttttactcct gtcatgccgg gtaataccgg atagtcaata
     6061 tgttctgttg aagcaattat actgtatgct catacagtat caagtgtttt gtagaaattg
     6121 ttgccacaag gtctgcaatg catacgcagt agcctgacga cgcaccgcat cacggtcacc
     6181 gctgaagcat tcccgccggg taatgccttc accgcgggca gtggcaaaag caaaccagac
     6241 ggtgccgaca ggcttctctt cactgccgcc atccggcccg gcgataccac taatagacac
     6301 ggcataatca gcacgagccg ctttcagtgc acctatcgcc atttccacca cgacgggttc
     6361 actcaccgcg ccatgctgcg ccagcgtctc ttcgcgtacg ccgatcatct gcgctttggc
     6421 ttcgttactg taggtgacaa atccgcgttc aaaccaggcg gagctaccgg caatatcggt
     6481 aatcactttc gctacccaac caccggtaca agactcggca gttgttacgg ttgcgccacg
     6541 ggctttcagc gcctgcccaa cctgttcact taactgcatc agttcactgt cagtcatcac
     6601 aacctctgtc agtcaaaaat ttatgccgga caagatagca ctttcgtcag gaagatggtg
     6661 ttggcgttgc aattttacga ggaggattca gaaaaaagct gattagccag agggaagctc
     6721 acgcccccct cttgcaaata gttactgcac gcgcgccagc gccacggctt gtcctaactg
     6781 ccagaccgcc attgcgtaat gggtgctgtg gttataacgg gtaatggtgt agaagttcgg
     6841 cagaccgtac cagtactggt aaccggtacc gacatccaga cgcagcaggc tggcttgttg
     6901 atggttgccc agcggctgct gtggcgttaa acctgccgtg gcaagctgcg aaatgctgta
     6961 cctggttttg aagccatttg gcaagcctgg agcctgaccg tttgccatta ccgcgacctg
     7021 atcgcctttc acccagccgt gcgctttgaa atagttcgcc acgctaccga tcgcatcaac
     7081 cggatcccac aggttgatat gcccgtcgcc gctgaaatct accgcatatt gtttgtaaga
     7141 cgacggcata aactgtacgt agcccatcgc cccggcaaag gaacctttca gattgagcgg
     7201 atcgtcctgc tcgtcgcgcg ccatcaacag gaaggtttcc agctcgccag agaaatactc
     7261 cgcgcggcgt gggtagttaa atgacagcgt tgccagcgca tcgaggatgc gagttttccc
     7321 catcacgcgc ccccagcggg tttcaacgcc gataatcccg acgataattt ccggcggtac
     7381 gccatacacc tgccacgcgc gattcaacgc atcttcatac tgattccaga aaaccacacc
     7441 gttctgcacg ttgtccggcg taataaattt tttgcgataa cggagccatg cgccgttcgg
     7501 acctgatggc ggtttcaccg atgtggttgg tgcctggtta tccatcagcc gcagtaccga
     7561 atccagacgc ttcgcctggg agagaatttc ctgcaactgc tgacgatcga aaccgtgttt
     7621 gttcaccatt ttgtcgatga actgctgggc attcgggtta ttagcgaaat cgccgcccat
     7681 ctgcatcaca ttgtgctgcg gctcaagcag gaagccgcca gacggcgttc cggtggtcgt
     7741 ttcagtctca gtaggttttg gcttgctgct acaggcggca agcaacacaa aaaggggaag
     7801 caatgttaca taacgacgct tgaacatgag gggtccattt aacagattca accaggggca
     7861 agtatggtaa agcatcacgc cccacacaag gaagcggtag tcactgcccg atacggactt
     7921 tacataactc aactcattcc cctcgctatc cttttactca aactttcaaa ttaaaatatt
     7981 tatctttcat tttgagatca aaataacact tttaaatctt tcaatctgat tagattaggt
     8041 tgccgtttgg taataaaaca ataaatcctg aaggagagaa caatgataga aaccattact
     8101 catggtgcag agtggtttat cgggctgttc caaaagggcg gagaggtgtt taccgggatg
     8161 gtgaccggca ttcttccgct gttgattagc ctgctggtta tcatgaacgc actgattaat
     8221 tttatcggtc agcatcgtat tgaacgtttt gctcaacgtt gcgccggtaa ccctgtttcc
     8281 cgttacctac tgttaccgtg cattggcacg tttgtctttt gcaatccgat gaccttaagc
     8341 ctgggtcgct ttatgccgga aaagtacaaa cccagctact acgcggcggc ctcttatagc
     8401 tgccactcaa tgaatggcct cttcccccat atcaaccctg gcgaactgtt tgtttatctt
     8461 ggcattgcca gcggtctgac aacgctgaac ctgccacttg gcccactggc ggtgagttat
     8521 ctgctggttg gtctggtcac caatttcttc cgcggctggg tgaccgatct gaccaccgcc
     8581 atttttgaga aaaagatggg cattcaactt gaacaaaaag ttcacctggc aggagcaaca
     8641 tcatgacgca tattcggatc gaaaaaggaa cgggtggctg gggcggcccg cttgagctga
     8701 aagccacgcc gggaaaaaaa atcgtctata tcaccgccgg tacccggcct gcgattgttg
     8761 acaaactggc acagcttact ggctggcagg ctattgacgg atttagagaa ggtgaacccg
     8821 cggaggcgga aattggtgtc gcggtaatcg actgtggcgg cacattacgc tgcggcatct
     8881 atccgaaacg acgtattccc accattaata tccactcgac gggcaagtcc ggtccgctgg
     8941 cgcagtacat tgtggaagat atttatgtct ctggcgtaaa agaagaaaac atcactgtag
     9001 tgggtgatgc gacaccacaa ccctcttccg tgggccgtga ctatgacacc agtaagaaaa
     9061 tcaccgaaca aagcgatggt ttactggcga aggtgggaat gggcatgggg tccgccgttg
     9121 cggtgctgtt tcaatctggt cgtgacacca tcgacactgt attaaaaacc attctgccgt
     9181 ttatggcatt cgtctcggcg ctcattggca tcattatggc ttctggcctt ggtgactgga
     9241 ttgcccacgg tcttgctccg ctggcgagcc atccactggg tctggtcatg ctggcgctca
     9301 tctgctcctt cccactgctt tcacctttcc tcggcccagg cgcagttatc gcacaggtta
     9361 tcggcgtatt gattggcgtg cagattggtc tcggcaatat tccgccgcat ctggctttac
     9421 cggcactgtt tgccatcaac gcgcaggcgg cctgcgactt catcccggtc ggtttgtcgc
     9481 tggcggaagc ccgtcaggac acggttcgcg tcggtgtccc ttctgtactg gtgagccgct
     9541 ttttaaccgg cgcaccgact gtactgatcg cctggtttgt ctccggtttt atctatcaat
     9601 agaggctgaa acatgaccgt tatttatcag accaccatca cccgtatcgg cgcgagtgcc
     9661 actgacgccc tcagcgacca gatgctcatc acctttcgtg aaggcgcgcc agcggacctc
     9721 gaagagtatt gcttcattca ttgccacggc gagttgaaag gtgcactcca tcccggtttg
     9781 caatttacgc tcgggcagca tcgctatccg gtgaccgctg ttggcagcgt ggcggaagac
     9841 aaccttcgcg aactgggtca tgtcaccctg cgcttcgatg gtttaaacga agcggaattt
     9901 ccgggcactg tccatgtggc aggccctgta cccgacgata tcgcgccggg atcggttttg
     9961 aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat cggtggtggg
    10021 caaaccttag gcgcgttcct gtgccacggt ctggctgccg aggggtatcg cgtcgcggtt
    10081 gtcgatattc agagcgacaa agccgcaaat gtggcacaag aaattaacgc cgaatatggt
    10141 gaaagtatgg cgtacggttt tggtgctgac gccactagcg agcaaagcgt tctggcgctc
    10201 tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc tggtctatag cgccggaata
    10261 gcaaaagcag cctttatcag cgacttccag ctcggcgatt ttgaccgttc gctacaagtg
    10321 aatctggtgg gttatttcct gtgtgcccgt gaattttcgc gtttgatgat ccgcgacggg
    10381 attcagggtc gcattattca gatcaactcg aaatccggca aagtgggcag caaacacaac
    10441 tctggctaca gcgcagcgaa atttggtggc gtcgggctga ctcaatcact ggcgctggat
    10501 ctggcggagt acggcattac ggtgcattca ctgatgctcg gtaacctgct gaaatcgccg
    10561 atgttccagt cactgttgcc acaatacgcg accaagctgg gtatcaaacc ggatcaagtc
    10621 gagcagtatt acatcgacaa agtaccgctc aaacgcggct gcgattatca agatgtgctg
    10681 aatatgctgc tgttctacgc cagtcctaag gcgtcgtact gcaccggaca gtcgatcaat
    10741 gtcaccggcg gtcaggtgat gttctgatca acagcggaga tccattaagg atctccgtga
    10801 gactatagaa tgcctgatgc gctacgctca tcaggcatac aggacttccg ccactacatt
    10861 aaggaaaagt tatggtatcc gcactcatca ccgtcgccgt tatcgcctgg tgtgcgcaac
    10921 tggccttagg cggctggcaa atttctcgtt ttaaccgtgc cttcgacaca ctatgccagc
    10981 aagggcgggt tggcgtgggc cgttccagcg ggcgctttaa accgcgggtc gtggtcgcca
    11041 tcgcgctgga cgatcagcag cgcatcgtcg acaccttgtt tatgaaagga ctgaccgtct
    11101 tcgcccgacc gcaaaaaatt cccgcaatta ccggtatgca tgcgggtgat ttacagcccg
    11161 atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg cttaaactga
    11221 aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt tgaaacctaa
    11281 atcaggtaat cacgcccatg aaacctcgtc agcgtcaggc cgccattctg gagtatctgc
    11341 aaaagcaggg taaatgctcg gttgaagaat tggcgcaata ctttgacacc acaggcacaa
    11401 ccattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt acttatggcg
    11461 gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaaca ctcatcaaca
    11521 cccacaagaa agagctgatt gcagaagctg ccgttagttt tatccatgat ggcgattcga
    11581 tcattcttga tgctggcagt accgttttgc agatggttcc cctgctctcg cgctttaata
    11641 acatcacggt gatgaccaac agcctgcata tcgtcaatgc gctatccgaa ctggataacg
    11701 aacaaactat cctgatgcca ggcggaacgt ttcgtaaaaa atcggcctca tttcacggac
    11761 agctggcaga gaatgccttc gaacatttca gtttcgataa attgtttatg ggcaccgacg
    11821 gcatcgatct caatgcgggc gtaaccacct tcaacgaggt ttataccgtc agtaaggcaa
    11881 tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt ggccgtaaaa
    11941 gccccaacgt agtttgcagt cttgaaagcg tcgataagct gattaccgac gcaggtatcg
    12001 atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata accggagaga
    12061 gcaatgagtg aagcactact gaacgcggga cgtcagacgt taatgctgga gttgcaggaa
    12121 gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa tatcatcctg
    12181 cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat tggtaagaaa
    12241 atcgccgcaa cacttgccag taccggcaca ccggcttttt ttgtccatcc ggcagaagca
    12301 ctgcacggcg atctggggat gattgaaagc cgcgatgtga tgctgtttat ctcttactcc
    12361 ggtggcgcga aggaactgga tctgattatt ccgcgtctgg aagataaatc tatcgcgctg
    12421 ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcaaaagc ggtgctggat
    12481 atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag caccgtcaat
    12541 accctgatga tgggtgacgc gctggcgatg gcggtcatgc aggcgcgcgg atttaatgaa
    12601 gaagattttg cccgctccca cccagccggg gcactgggcg ctcgcttgct gaataaagtg
    12661 catcatctga tgcgccgtga cgatgccatc ccacaggtgg cgttaaccgc cagcgtgatg
    12721 gatgcgatgc tggaactcag ccgcaccggt ctggggctgg tggcggtatg tgacgctcaa
    12781 caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt tggcggcggc
    12841 gcactcacca cgccagttaa tgaagcgatg acgacaggcg gcaccacatt acaggcacaa
    12901 agtcgcgcca tcgacgccaa agagatcctg atgaagcgca aaatcactgc cgcaccggtg
    12961 gtggatgaaa acggcaaact caccggcgca attaacctgc aggatttcta tcaggccggg
    13021 attatttaat ccttcaatcc cagacgtttc gccagccgat gcaggttggc gacgtcggtt
    13081 tccagcatcc gcgcgcaggc agcccagttg tgatgatttt gtgccagtgc ctggcgaatg
    13141 gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac gggcaccgcc
    13201 gccgcttctg gcggcggcaa cgtcacctca ggaaaagcaa aatgttgtgc ctcaagaatc
    13261 acttcatcgc cgctgcgagt ggctctcgcc agcactaccg cccgatgaat agcatgttcc
    13321 agttcgcgca cgttccccgg aaaacgatag tgttgcagta aatttcgcgc tccggcactt
    13381 aataccacgc gggagagccc cagccgcaaa cgacactgct cgcagaaata ccccgccagc
    13441 agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa cacgctcagg
    13501 cgatgaaaca aatcggcgcg gaatcgccct gccagcacct cttcgcgtaa gtcgcggtta
    13561 gtcgccgcca gcacgcgcac atcgacccgc aaactacggt catcgccaac gcgctgaata
    13621 tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa ctcgccgatc
    13681 tcatcgagaa acagagtgcc gttatccgcc atttcaaact tcccgctgcg gttactgata
    13741 gcgccagtaa acgctccttt cacatgcccg aacaactcac tttccgccac actttccggc
    13801 agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc ttcatgaatc
    13861 gctttcgcca ccagctcctt accggttccc gtctcaccgc tgatcagaac gttgagatcg
    13921 gacgccgcca caatctcaat ctcttttttc aattgcgtca tgcctggcga caagccaatc
    13981 atctgcgtct gtttcaccgc ttcaaacggc gcggcatcgc ccggcagcat attctggctt
    14041 tccagttgtt caatcagcaa cgcattgctt aacgctcccg ccgccagcgc ggcaatcagc
    14101 cgtaactctt cgtcgctgaa aacatcgaac tgatcgggct gcatcccgtc gagcgtcagt
    14161 gcgccgatca ggttttgccc ggcaaacaat ggcagaccaa cgcaggcgtg aaccttcaga
    14221 ctctcctgcc caggaatcaa accgtcatag ggatcgggca attcgctgtc tgcaggaaag
    14281 cgcaccacgt ccccggcgcg ggcaatcgct tcaagccgtg gatgcccttc cagcgcaaag
    14341 cgtctaccga gtacatcctt tgccagaccg tcgatggcaa gcggaataaa ctgccgcgaa
    14401 tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgtagcgt ggtgatcagg
    14461 cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat attcgccagc
    14521 acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat attgtcatat
    14581 cgaccatttg attgatagtc attttgacta ctcattaatg agcataattt tatttataga
    14641 gtaaaaacaa tcagataaaa aactggcacg caatctgcaa ttagcaagac atctttttag
    14701 aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac attcattggg
    14761 ttggtcaacg tgactgggaa gtgcgtgatt ttcacggcac ggaatataaa acgctgcgcg
    14821 gcagcagtta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc gacaccgtcg
    14881 accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat ctggcggata
    14941 tcgattacat cgtgattaac catgcagaag aggaccacgc tggggcgctg accgaactga
    15001 tggcgcaaat tcccgatacg ccgatctact gtactgccaa cgctatcgac tcgataaatg
    15061 gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac acgctggata
    15121 tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg ccggacagca
    15181 tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc ggtcagcact
    15241 actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttc gagcagtgcc
    15301 agcgttacta cgccaatatc ctgacgccgt tcagccgcct ggtaacgccg aaaattaccg
    15361 agatcctggg ctttaactta ccagtcgata tgatagcgac ctcccacggc gtggtctggc
    15421 gcgataaccc gacgcaaatt gtcgagctgt acctgaagtg ggccgctgat tatcaggaag
    15481 acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg gctgacgcta
    15541 tcgcccaggg aattgcggaa accgacccac gcgtggcggt gaaaattttc aacgtcgccc
    15601 gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg ctggtcggca
    15661 cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag gagatgactg
    15721 gtttacgctt ccgtaacaaa cgcgccagtg ctttcggctc tcacggctgg agcggcggtg
    15781 cggtggatcg tctttccacg cgcctgcagg atgcgggttt cgaaatgtcg cttagcctga
    15841 aagccaaatg gcgaccagac caggacgctc tggagttatg tcgtgaacac ggtcgcgaaa
    15901 tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg gtggttaaag
    15961 aagaaacctc tgccaccacg acggctgacc tcggcccacg gatgcagtgc agcgtctgcc
    16021 agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca ggaacgccgt
    16081 ggagtgaagt tccggataac ttcctctgcc cggaatgctc cctcggcaaa gacgtctttg
    16141 aagaactggc atcggaggca aaatgagtaa cggcattgtg atcattggtt cgggcttcgc
    16201 cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc actattccat taaccctgat
    16261 tgccgccgac agcatggatg agtacaacaa acctgacctc agccatgtta tcagtcaggg
    16321 gcaacgtgcc gacgacctta cccgccagac ggcgggtgaa tttgccgagc agtttaatct
    16381 gcgcctgttt ccgcacacct gggtgacgga tatcgatgcc gaagcccgtg tggtgaaaag
    16441 ccagaataat cagtggcaat acgacaagct agtgctggca accggtgcca gcgcctttgt
    16501 cccgccagtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag agtatcgcgc
    16561 ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gtcggtggtg gcttgattgg
    16621 tagcgaactg gcgatggatt tttgtcgtgc aggcaaagcg gtcacgctaa tcgacaacgc
    16681 tgccagtatt ctggcgtcat taatgccacc ggaagtaagc agccgcttgc agcatcggtt
    16741 gacggagatg ggcgttcatc tgttgttgaa atctcagttg caggggctgg aaaaaacaga
    16801 ttctggcatt ctggcaacgc tggaccgcca gcgcagtatc gaagtggatg cggtaattgc
    16861 cgccaccgga ctgcgcccgg aaaccgccct ggcacgacgc gccgggctga cgattaatcg
    16921 cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc gatatttacg cgctgggcga
    16981 ttgcgcggaa attaacggtc aggtattgcc gttcctccag ccgattcaac ttagcgcgat
    17041 ggtgctggca aaaaatcttc tcggcaataa cacgccactg aaactaccgg cgatgctggt
    17101 gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc gtcaggattt
    17161 acgctggcag attaataccg aacgccaggg aatggtggcg cgcggcgttg acgatgctga
    17221 ccagcttcgc gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg gattgttgaa
    17281 aacgttgccg atgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg ctggcgcgtc
    17341 ttatccaacc tacggggagc gcatgtgttg gtcacatact gcgttcatgt cgcatccacc
    17401 tactgtgccc aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta cggggacgca
    17461 tgtgtaggcc ggataaggcg tttacgccgc atccggcaat ggtgtccaaa tgcaacacgt
    17521 tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc ccctgcccca
    17581 gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga
    17641 gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg ctaaatacca
    17701 gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca
    17761 gcgcatcatg aaacgcccac gcgcgttgat taaccggtgc ctgccagttc agccactgct
    17821 gccagaaagt ggcgagatcc agttgattgt ccaccagcgg cattgtcacc ggatgcgtca
    17881 ctccgtggca cgaagctgcg agtgcctcca gagcacaagc cgcttcacct tcataactta
    17941 acgtggctgg cgcacagccc agtgccgccg ccacggcatc gaacaaccgc ccacacgatg
    18001 acgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagcacgctc cagttttgct
    18061 gttgcacact tgctgtttca gaataattct gccactccgg cacaaagcgc aggcactgcg
    18121 ccagcaggtt tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc gccactgcag
    18181 gcaagccgcc caggtgctca cattcgcgat agttcacccg cagacactcg ccgccccaca
    18241 aagcgccgtt ctcccccata ccgataccat cgagcgtcaa agcaatgaca tccccgccat
    18301 ccagcggcca ctgatgctct gccagacacg ccgctgcgtg ggcatgatga tgcagcaccg
    18361 tttgcgtcgg catattcatt tcgctcgccc actggctgga gacatagccc ggatgcacgt
    18421 catgcacaac gtattgcgga gtgaaatcgt agatgttttg catcaggcgt aacgcttcgc
    18481 gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc aaaaccgctt
    18541 gttcaccgcg caccaggcag aaggtgtttt tcagatccgc gccgagacac agcacaggcg
    18601 gaacattttt aaagcccgga ggcaaagcca gcgcatccgg cacatacccc cgcgaacggc
    18661 gcagcatttc gccgctttcg cgcaccaccg aatcatccat ccgctgcacg atgtcgcggt
    18721 tatgtatcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga
    18781 tagctggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat tgcagttcct
    18841 gtaacagcaa atgctggagc gggttcgcag gcaacattac cccgacttcg ttaaggtcag
    18901 gggcgatatc atcacaaagc tcaggaacgt attttttatc caccagcaca atcggcgcgg
    18961 cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaacc gtcagccact ggcaacatga
    19021 ctgccagcgg tttcgccggg cggtgtttgc gctcccgcag tgtcgccacc gcgttactgt
    19081 tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca attttgccca
    19141 tttttaactg tgcgatagct gcctgtaatg ccgcctcttg ttccgcatgt tcaccatgac
    19201 ttacccattc aagatgcggg ccacactccg ggcaggccac cggctgggcg tggaagcgac
    19261 gatcgagcgg gtcgcggtac tctttatcac aggctggaca cagcggaaac tccgccatta
    19321 cggtaaacgg gcggtcgtaa ggcatggcgc gaataatggt gaaacgcggg ccgcagtggg
    19381 tacagttgat aaacggataa cgataacgcc gttcgcctgg ggtattcatt tcggcaaggc
    19441 aagcagggca agtagcggca tccgggacaa tttgcgtatt catggcacct cccgcgctct
    19501 ggcggatagt gaactcggtg ggcagttgtg accagataaa cggctcacgc tcgacgctat
    19561 caatacgcgc cagtggcgga cagtgctgat gcaattgaac aagaaacgtt tccgggtctt
    19621 ccagcagccg gacttctacg ccatcgccgt cattacagac atcgccgtga agatttaatt
    19681 gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg ccacgaatac
    19741 gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca tcatgaattg
    19801 cgtaacccgt ccagccggac acgacagcgt cgcatccggc agtcacaggt cggcgatacc
    19861 gcccgcttcg tattctacga atatttccgg gaattccttt gatgccagaa cagttctgta
    19921 agatttttag aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt gttcaagttt
    19981 attacgatcg acacaaatta gcgcatgggt cgggcaagcc gccatacacg ccgggccgtc
    20041 ttcacgatgg ttgcacaggt cgcatttatt ggcttcagct ttgtcagccc gtacattcag
    20101 ccccacgccg ctgttgcgaa tcaccggacg taccaccact tccatcgcgc catacgggca
    20161 agccaccacg caggttttgc aaccaataca acgttcctgc atcacatgaa caaacccttt
    20221 atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt cacactgacg
    20281 gcaaactgtc gccgtggaaa tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt
    20341 ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc acgccacttc
    20401 acaggtacgg caaccaatac atttactcgc gtcagcaatg atgaaacggt tcatcaaatt
    20461 ctccagcaat gacagttaat gcgccgatac attcacaaat catgccagtt tttaatttac
    20521 tgttatttaa ggaaattaat ttctgtaatg caggaaaaac gatgtcatcg acactagtga
    20581 cgatgacatg tgatgacaat gtttatcgcg aaagggcaat gagtgagtcg cggcagatca
    20641 attttccgct gaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc
    20701 gtccaataat ttcctgaatc atctcagtca ccggaatttt tacgctggag agcgccggaa
    20761 cgatataggg ggcaattgcg atatcatcga atccgataac tgacacctgc tctggcaccg
    20821 ctacgccgcg ctcgtgcaac gctttcatcg cacctatcgc catatcgtcg ttactggcaa
    20881 ctaacgcgct aaatttagcc ccacgttcga gcaacatttc taccccttcg gccccgctgg
    20941 caggcgtcca tctaccgtta gcgataagtt tttcattgag cgcaataccg tgctgcgcca
    21001 gcgcgtcttt atatccggca agacgttcaa cgctggtggg agaatccatc gaaccggtaa
    21061 ggaaagcaat ctcctgatgt ccggcgttta tcaactctgc cacggcgtta aaactggtct
    21121 gtttatgatc gcaccagacg ctatggctgc tgtttttgcg caggcggcga ttaagcacca
    21181 ttatcggctg actgtgcgcg tcaatgatgt catcgatctc atccacgctt aaaaaacgcg
    21241 ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt
    21301 cttcggcgct gtgtttacca tctgccaata gcaactgccg ccctttctct tccgccattc
    21361 gcgcggcatg aaacagtaat tcactaaaat aaatgccgtg gtaaagcgtg ttggtcacta
    21421 ccagccccag cgtctgagta ctcttcgccg acaaattgcg cgccagcaag tttgggcggt
    21481 aaccgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat
    21541 tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca
    21601 gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt
    21661 gccgattgtc cttgcgcaat cggcgggaaa aatattcagg tgaccggttt cacaaatata
    21721 aaaaatgaac aattaactct cttgcttatt tagtgacaac tattcatgat tttgtgaaac
    21781 cggtttccta attccgtttc agcatcggca tttttccgtt acgtcgactg ataacaacta
    21841 catctaccct actgataaca ggataaaatc cgatggccaa aaattatgcg gcgctggcac
    21901 gctcggtgat agcggcactg ggcggcgttg ataacatctc ggcggtcacg cactgtatga
    21961 cgcgtttgcg ctttgttatc aaagatgacc agcttatcga cagcccgacg ttaaaaacca
    22021 tccccggcgt gctcggcgtg gtacgtagtg acaaccagtg tcaggtgatt atcggcaata
    22081 ccgtttcaca agcctttcag gaagtcgtca gcctactgcc gggagatatg cagcccgcac
    22141 agcccgtggg taaacccaaa ctcacgctgc gtcgcattgg tgcagggatc ctcgatgcgc
    22201 tgatcggcac catgtcaccg ctgatcccgg cgattatcgg cggatcgatg gtcaaactgc
    22261 tggcgatgat cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc ttaaccattc
    22321 tgaatgtgat tggtgacggt gctttcttct tcctgccgct gatggtcgcg gcatctgccg
    22381 ccatcaaatt taaaaccaat atgtcgctgg cgattgccat cgcgggtgtg ctggtacatc
    22441 cgagttttat tgaactgatg gcgaaagcgg cccagggtga acatgtcgaa tttgccctga
    22501 ttccggtcac cgcggtgaaa tacacctaca cagtgatccc ggcgctggtg atgacctggt
    22561 gcctgtcata tatcgaacgc tgggtggatc gcattacacc ggcggtgacc aaaaacttcc
    22621 tcaagccaat gctgattgtg ttgattgccg caccgctggc aatcctgctg attggcccga
    22681 ttggtatctg gatcggtagc gccatttcgg cgctggttta caccattcat ggttatctgg
    22741 gctggctttc agtcgccatt atgggcgcgc tgtggcctct gctggtaatg accgggatgc
    22801 accgcgtctt tacgccaacc atcattcaga ccattgccga aaccggcaaa gaagggatgg
    22861 tcatgccgtc agagatcggc gctaacctgt cgctgggcgg ttcatcactg gcggtggcgt
    22921 ggaaaacgaa aaacccggaa ctgcgccaga cggcgctggc tgcggcggca tcagccatta
    22981 tggcggggat ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa cgtccgctta
    23041 tcgccagtct tatcagcggc tttatttgcg gcgcggttgc cggtatggcg gggcttgcca
    23101 gccactcaat ggcagcgccg gggctattta ccagcgtgca gttcttcgat ccggcgaatc
    23161 caatgagcat cgtctgggtg ttcgcggtca tggcgctggc ggtcatgctg tcgtttatcc
    23221 tcacactgtt gctcggcttt gaggatattc ctgttgagga agcgactgcc gaggcgcgaa
    23281 agcatcagag cgtacaaccg accgtcgcca aagaagtaag tcttaattga ggatgaaaat
    23341 gtcagtattt ccagaaggtt ttttatgggg cggcgcgctt gccgccaacc agtctgaagg
    23401 tgcgttccgt gaaggtggca aagggctgac cactgtcgat atgatcccac acggcgagca
    23461 tcgaatggcg gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg acgagtttta
    23521 tcccagccat gaggcgacgg atttttatca tcgttataaa gaagatatcg ccctgatggc
    23581 agagatggga ttcaaggttt tccgtacctc aattgcctgg agccgtctct ttccgcaggg
    23641 cgatgaaatc acgcccaatc agcagggcat tgctttttat cgttctgtct ttgaagagtg
    23701 taaaaagtac ggtatcgaac cgctggtcac gttgtgccac ttcgatgtgc cgatgcatct
    23761 ggtcaccgaa tatggctcct ggcgtaaccg caagctggtg gagtttttca gccgctacgc
    23821 ccggacctgc tttgaagcat ttgatggtct ggtgaaatac tggcttacct tcaatgaaat
    23881 caacattatg ttgcatagcc cgttctccgg cgcgggtctg gtgtttgaag aaggtgaaaa
    23941 tcaggatcag gtgaaatatc aggccgcgca tcaccagctg gttgccagtg cgctagccac
    24001 caaaatcgcc catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg cgggcggtaa
    24061 cttctaccct tacagttgca agccggaaga tgtctgggcg gcgctggaga aagaccggga
    24121 aaacctgttt tttatcgatg tgcaggcgcg gggcgcgtat ccggcttact ctgcccgcgt
    24181 attccgcgaa aaaggggtaa ccatcgacaa agcaccgggc gatgatgaaa tcctgaaaaa
    24241 caccgtcgat tttgtctctt tcagctatta cgcctcgcgc tgcgcctcgg cggagatgaa
    24301 cgccaacaat agcagtgcgg cgaacgtagt gaaatcgctg cgtaacccgt atctgcaagt
    24361 gagcgactgg ggctggggta ttgatccact cggtctgcgt atcaccatga acatgatgta
    24421 cgaccgttat cagaagccgc tgtttctggt ggaaaacggc ctcggcgcaa aagatgaact
    24481 tgctgccaat ggcgagatta acgatgacta tcgcatcagc tatttacgcg aacatatccg
    24541 cgcaatgggc gaagcgattg cagatggcat tccgctgatg ggctacacca catggggctg
    24601 tattgattta gtttccgcct ctacgggtga aatgagcaaa cgctacggtt ttgtctacgt
    24661 agaccgtgac gacgcaggca acggcacgct gacgcgcaca cgtaagaaat cgttctggtg
    24721 gtataaaaaa gtgattgcca gtaatggtga agatttagag taagcaacag tgccggatgc
    24781 ggcgtgaacg ccttatccgg cctacatgtc gcacgaacca ggaaggcccg ataagcgcag
    24841 cgcatcgggc aatctggggt ttggcatcag tcaaaatcaa agaaaccatt ccggcttaac
    24901 gccaaatcgg gctgaaagtg ccttaatgtg ggatatcgtc agggatcgtt gtccagacaa
    24961 aatctgactg accagggatt tagagccgat ttcgttcttc aaatccgcat aggtcagatt
    25021 atgttgatca atcagcgtgc gaagtaacgc cacgccaaca ggcattgccg caacggcttt
    25081 gtcgaactct gcaaactttt cgttgttgtt ttcatactct gcaatgcggc tcgccagaaa
    25141 gtcgataagt ggattggtgt catcttcttc aatcaaatat tcgaccagtt ccagtgcttc
    25201 acgataatca tcttctgagt cactgcctcc cagaaagggg actgcattga ccagttctct
    25261 tgttgcttta acagcgcgtg cagcattagc agtcattctt tgttctctct gtagtaacgt
    25321 gtcagtttgt catactctgc gtgattcgtt atgtgcttca caaagaaccg cttattaaca
    25381 aagtttatgt aggcaataac cctaagattg ttcccgccga catccagaac gtaccatttg
    25441 ttgcgatatc taaaattgtc gaggttggga aacgccgttc gcatttcttc tggtgtagaa
    25501 aaatccgttt ccttaataac ccgatacaat gcctgatgag ccagagcgtc gttaggatat
    25561 ttacgcgcag actcttcaaa tggcgcttta gaaatgatgt gcatttttaa gttttaactt
    25621 ccttatagta agtaaacagc cacatctgcg atgttctatc cttatgtgtt cggaaactat
    25681 agctcatcaa atccaagttc acaatatgta aacacttgtg cgcgatataa ttacccccaa
    25741 aatcctcata gccactctgc aaaaagtcag ttctggttca cccctcaagc acatccccct
    25801 ttatcaagca acgctgcgtc tgcgccagat cttccgtttg cgggtcgcgc ccggcgatgg
    25861 gtttgaggac gagtttacgg ttgggggtat aagtaaaacg cgtgagcgcg ggatagctta
    25921 acttgccaaa ccagccgtga acacgctgcc atttggcgtc ttttacctga tacagactgg
    25981 ttatccagta accagaatta aactgcatat ccagcaattg cgtgtgacca ttgccttgca
    26041 aatccagcaa atcatctacg ccagttttgc ttgcggtgta gaatcccctg ggttcaaaga
    26101 cacagggacg gccttcgcgc gtgaacgtca taagaattaa atgtgcataa ggagccagac
    26161 cattaccggt acttggtaac cagataacca gatcctgaat gccgttacga tcaagatcgg
    26221 cgcgatagag ttgccctttg cccgctatgt ttgccagcat acccacaggc acagtccagg
    26281 atttaccggc gcggtcttta gcggttaatt gccagtaatc ttcttcattt ggcatcttct
    26341 caagcgtcac gccagcaacg cccactttcc taaaatgcca ggtttgctcg gcatcataag
    26401 gcatggtcag gagaggtttc agcgcccgct tttgcgcgta ttcgctgtca ctcattggca
    26461 gatattcggt aaagctggtg gcctgtgcag gcagcactaa gctcaataaa aaagcgcaca
    26521 tccctgttct gatgttcaat tccctgaatt ccctgatgga gaattattct tcttccaccg
    26581 ctaactgcgc aaatccacca tttccttccc agccttccag tcgttgataa acggtttcta
    26641 ccgcatcttt aatcggctgg gtcatcgggt agtaaaagcc gacgatatcc ggctgaatgc
    26701 cgaggaaaat cacttcacca atgtcttctt ttaactggtc gataaggtaa ttcaacggca
    26761 tgttatgggt agtcatcata aacatctcgg cgatatcatc cgggtcgatg atgcggattt
    26821 cgccggggtt tagccccata tccgtggcgt cgacaatcag cagtcgtgtc gggcgcagtt
    26881 cacggatggc gacgatgtcg ttttccggtg cactaccgcc gtcaatcacc acccagttac
    26941 ctttcggcgc ggcggcgcac ttttccgcca gcagcgggcc agcgccatca tcgcccatca
    27001 tgctattgcc aacacagagt aaaacgtcag tcacgcagtc tcctcaccat caaatagatg
    27061 gcgttttcct gatgaatatc atgcagcata cttaacagca ttgtactcca ttccttctgc
    27121 tgcgaagttt gcgctgcccg ggctttatcg aatgcgttgg ccagcatcgg cacatggtta
    27181 atgtcgatga cgatctcgcc atatttcggt acgccttcca ttttgcggcg ggcttcgctg
    27241 cccgcctcca gagtggcaat ccatgccaga tattcatccc atgggcaggt gagcgccgct
    27301 tccaggcaat cgataacccc aaggtggtga ccaatcgcca ggctgtaata gaccacctgc
    27361 tgcgcctcgg cgggcgtggc atcgttctca tcaataaatt tacggctcag ttgactgaac
    27421 accacctttt cactcatcgg atacgcgcct cttcaacaac atgattcaga tggctgacaa
    27481 tctcgttcag acgcggatcg ttttccgctt ccagccagcg tgcaacctgt tcttcgccct
    27541 gccctaactg tgtaaggtaa tcatcggcaa tctgacgacc gtaacgataa cccgccaggc
    27601 gacgtgcttc gcgatccact ttcacgcgca gcggctgcac catatcgcca tgcagaatct
    27661 cagccggttg ttcatcctgc tcacccgggc cacgggcgtg aattttctgc tccagcaggc
    27721 cgagtgccat tgcaaagccg tacagcgtgg cggcaggcgt tggcgggcaa cccggaatgt
    27781 agacatctac cgggacgatt ttatccgtac cgccccacac gcagtagaga tcgtggaaaa
    27841 taccaccgct gttaccgcag gcaccgtagg agatacaaat tttcgggtcc ggcgcggact
    27901 gccacgcacg cagcgcaggg gatcgcattg cacgggtgac cgcgccggta aacagtaaaa
    27961 tatccgcatg acgcggtgaa ggaacgactt taatgccgaa gcgttctgca tcaaacagcg
    28021 gcgataaagt ggcgaaaatt tcgatttcgc agccgttgca gccgccgcag tccacgcggt
    28081 aaacataggc agaacgtttg atttttttca gtaacgacgc cttcatgctg gcgatggatt
    28141 catccaccgt catcgggacc ggaatgccgt tggcgtcacg ggggcctaat aaattgctca
    28201 tcagatggcc tctttcatat ggcgagtcag ttcaatacgg tcggacggca ccaggcattt
    28261 ctggcgctta cattccgggc aagtctcaaa gctttcgcgg tggttttccg cgcggctgtc
    28321 accattgtgc ttaagcagcg caatggcgta gtcgatctct ttctggacgg cgaaaggacg
    28381 attgcagacg cgacagctgc acagcacaaa acgggactgt tgcaggaaat cttctttctt
    28441 ccacaccgcc agttcgtact cttgcgacag tttgatcgct gccgtcgggc agacttcttc
    28501 gcagcgtcca cagaagatgc agcgcccgag attaaactcc cacgccagct caccggtggc
    28561 gaggtcagtt tcaaccgtta aggcgtttga cgggcaggca ttgacgcagg ccgcacagcc
    28621 gatgcactgc tgcgggttct gctccggctt accacggaag tttttatcaa ccgcaatcgg
    28681 ctccagcgga taagacgagg tcgccgtgcc ggttttgatg acttttttga taaaggtaaa
    28741 catggcgatt ccttatttca gcggcgagtt tttacgctca atgctgtaac gctcgagttc
    28801 tttgtacggc accactttgc tctttttctt acgcacatcg accacggtca tgcggtcggt
    28861 acaggagtag caagggtcca ggctaccgat aatcagcggc gcatcggaaa cggtgttacc
    28921 gcgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc gcggcacggc agcgccagcg
    28981 gtacagcttc tggttgtcgc cggtcatgct ccagtggata tcgtcgccgc gcggcgcttc
    29041 ggcaaagccc agcgcgaaac ggtgcggaat gtaggtaaag ccttccacca tcagcggacc
    29101 gcccggcagg ttatccagac cgtagtcgat catgttcagc gcggtataaa cttcgttgat
    29161 acgcactttc agacgggaaa taacgtcgca gccctgctcg ctgtggactt ccattggcag
    29221 caggccataa ccgacaaacg ggtgatcggc gcgagtatcg cgggcgtgac cgctggcgcg
    29281 aaccatcggg ccaacgttac tgaaatcgcg ggcgatttcc gggtccagac gaccaatgcc
    29341 gacggtgcgc tgttccatgt tcggcgtact cagcagcaca tccaccagct cctgcacttc
    29401 acgacgcatc tgttgcgcca gctggcgggt ctggatcatg tcatctttca acagatcgcg
    29461 acgaatcccg ccgatcaggt tcaggccgta ggttttacgc gcaccggtga ggatctctgc
    29521 cattttcatg gaggtttcac gcacgcggaa gaactgcata aagccggagt caaagccggt
    29581 aaagtggcag gcgagaccga ggttgagcag atgcgagtgc aggcgttcca cctccagcag
    29641 aatggcgcgg atcatctgcg cacgttctgg cacctgaata cccatcgcgt tttctaccga
    29701 cgtggtgtag gcggtgctgt gggcaaagcc gcagatcccg cacacacggt cagagaggaa
    29761 ggtcacttcg ttgtaaccca tacgggtttc cgccagtttt tccatgccgc gatgaacgta
    29821 gaacagacgg tagtcggcgt cgataatgtt ttcgccatcg acgaacagac ggaagtggcc
    29881 cggttcgtca gaagtgacgt gcagcggacc aatcggcacg acgttgtttt tcttgtcgcc
    29941 cagttcgttg atgaactcgt aggtttcagc atcggtagtc ggtgccggac gctgacgata
    30001 atccatgctg tctttacgca gcggataaag ttcatccggc cagtcatccg gcagcaccag
    30061 acgacgttca tccggcagac caaccggaat caaaccgtac atatcgcgca cttcgcgctc
    30121 gccccacacc gccgccggaa cgcgcggcgt cacggacgga tactccggtt tgttggcgtc
    30181 aacttcgacg cgaaccgtta cccaacactt ggtgcccttc tccatcgaca gcacgtaata
    30241 aacggcgtaa tgaccattca gtttgcgttc gtcgttacca aacagcaccg acagccagcc
    30301 accctgcttg tagtagagaa actccaccac ttccggcagg tagttcacct tcacggtcac
    30361 ggtcagctga tctttggtct gccaggcgtg gtccagcacg acgcccggaa atgcctcatt
    30421 cagcgcggcg agataatgtt gacctaattt ttcttcagac atgctcaaac tctctttaat
    30481 cacgccgcca gcaaggagac gaacgctaaa aatgcaaagc caaacccagc ccaggtaatg
    30541 cgcggagtaa tatcaagacg cagacgggcc atgctgtttt cgaacagcgc gataaccagg
    30601 acgccgacga ccagtttgac gatggcaatc accagcgcca gcagcagccc accggcggtg
    30661 aaggtttcca tctgtcccca cgggataaat acgccgacga acatctgcag caccaccagc
    30721 tgtttcaggc tgatacccca tttcatgacg ccaaagccgc tgccgctgta ttcagagagc
    30781 gggccttcct gcagctcctg ctcggcttcc gccaggtcga acggcagttt gcccatttcg
    30841 ataaaggtgg cgaacgcaca ggcacaaagc gccagtacca gcgggatgct ctggctcagc
    30901 ggccagtgat aaacggtgtc ggtgatgttg ctgatgttgg tggaaccggc aacctgtgcg
    30961 gcaacccaca gaccaagcag cagcatcggt tcgaccagca cgccaagcat cgcttcacgg
    31021 ctcgcgccga tagcggtaaa cgggctaccg gtatccagac cagaaatggc aaagaagaaa
    31081 cgcgcgatgg caaagagata cagtaaggtg attaaatcac ccagttgcgg cagcggagaa
    31141 ccgacggtca ccaccggcag cgcagtagcg atagtcagca tgacgcccac catcacatac
    31201 ggcgtcaggc ggaacaccca gccggaggca tccgggccaa cgctctggcg gcccagcagt
    31261 ttgataatgt cacgatactc ctgcaacaca cccggcccac gacggttatg taagcgggct
    31321 cgcgccacgc gggtaatacc ggagagcagc ggcgcaacgg caaatagcac cagcgcctga
    31381 attaacggat ataaaacact cattctcagg ctcctcgtga aacaataatc accaccagta
    31441 ccgccagttc aaccagcgcc atccggcgga acagcaacgc actcccctcg cactgccagc
    31501 ccggcaccag agacaccgga ttcagccatt tgcgtagttt cagcaccggc gcaaacgcct
    31561 gtttcaccgg catggcaaaa ccgtgagcgg taatcaccat tgatttttcg tggtcgtagc
    31621 cgcacaccca ggccgcgccg cgggaacgcg atggcaaacg atcgcctttg caaatcgcca
    31681 taatgatgaa tggcagcagc ggacaggcaa tcagcagcaa tgtgatcatt ggttgagaaa
    31741 cggtggtgtt agcaggctcc agcggcagag gtacagcagc agacagcatc ggcagcagcc
    31801 acggcgcagc aacaccgcca attacgcagc aaatcgccag tgccactacg cttaccctca
    31861 tcaggagcgg cgcacaggtg gcgttttcgg cttctttggt acgcggcgcg ccgaggaacg
    31921 tgacgccata gactttcgcc atacacatca ccgccagcgc accggtaatt gccagaccca
    31981 cagcgagcag cggccccagc agacgggcaa caaacgcgcc gctattgctc agtttgaaga
    32041 aggattgata gataacccat tccccggcaa acccgttcag cggcggcagc gcagccattg
    32101 ccattaaccc gactaacatg gcgatggaga taaccggcat tttcttgcca ataccaccga
    32161 gtttttcgat atcgcgatga ccggtacgga accagacgct ccccgccccg aggaacagta
    32221 cgcttttgaa caggctatgg ttaagcagat ggtacagacc accgaccagg ccaagagcaa
    32281 tcagcgccgg ttgttcgagc gcgatacccg ttacgccagc gcccagcccc agcaggataa
    32341 tgccgatatt ttccagggtg tggtaagcca gcaggcgctg gatgttgtgc tccatcagcg
    32401 catacagacc accgacaaac gcggtgatca tgccgagcac cagcagcgcg atcccccacc
    32461 acagcggtgc attaccgccc agcagtgaca gggttaaaat gcccagcagg ccaattttca
    32521 tgactaccgt agaaaacaac gcggcagctg gcgcagaggc gttagcatgt gcctgcggca
    32581 cccagccgtg cagcggaata atcccggcga gcaggccaaa gccaatcaca ccgagcagcc
    32641 agatatcgga accgagcggc agctgttgca tacgcatatc cagcaggcgc agatccagcg
    32701 tgccataacg ctgccacagc agccagcaag caatcgccag cagcagagtg ccaagacgcc
    32761 ccagcgcaaa ccacagtttg ccctctttgc tgttgctagt gaggaacacc gcgcacaggg
    32821 ccatgatttc ggccattacc acgaacatgc cgaggttgct ggcaatgacg gcgcagacgg
    32881 cggcagccat caacatattg atctgcaagc cgttgcactt cacctgcgcg tgacgatgcc
    32941 agtcaatgtt atagaggctg acaaacagac cgcaaaggcc gagggtaatc agccaaatcg
    33001 cgttaagcgg ggagatttgc acatcgtagc ttaccagcga cagcgcaccg ctcacgccaa
    33061 ccgcgccagt cagcacagtg aagcccgcgg ctgccgtata caggctacca accgcgccgc
    33121 caattccggc tatccagcca cttagcgtct tttgaaaaga aaagagaaat gccagaacag
    33181 cggcggcgac aaaccacgcc acgccgctat tgatcaggga aattgcgctc atttagcctc
    33241 tccactttga gcctgctgaa acaaggtgag atcgccaaag tccgtgttaa aggtcagctc
    33301 acgcttacgt ttgctgacgc gggcgatatc ggtgttatcc accagatgca gggctttagt
    33361 cgggcacatc cgcacgcagg ccggaccttg ttcatcaaag ctacaaaggt cacatttgac
    33421 ggcgatcgcg cgaatacctg gcacccagtc aagcaatgtg ctgacacgcg ccggagcagg
    33481 cggtgccggt ggcgctttcg gggtattggc gtttgccgga atatccagcg gacggctgcc
    33541 ggaaaattca attgcgccaa acgggcaggc gatgccgcac agcttgcagc ttacgcacag
    33601 gctttcattc aactgcacgg ccccatcaac gcgggtgatg gcgttaaccg ggcagaccac
    33661 cgcgcagggt gcatcttcac agtggtgaca gagctgcggc gcagattctt tttcattcag
    33721 catcactctc aggcgcggca ttgattgcag gccgtgctgg cgatgcgtct ctgaacaggc
    33781 ggcctcacaa gtgtggcagc cgatacagag cgtggagtca gcaattacaa aacgattcac
    33841 caggcattcc tcaggtgatt gtcatttttg acgaaaacat gccgatgaaa tgtcattttc
    33901 gacactcatc gacacgccca tccccaaaca ggcgtaacgc ctgcaaaacg ggcaaagcct
    33961 cagctcatgc tgccgggctt tgtcccttta ccagttggct taaattcacc ggcacattgt
    34021 tttcaatagc ggtgtataag ctgtcgtaaa cgccagcgat tttttcgaga tagtgttcca
    34081 gaacctgttc gcgatcgcgg atgttgacct tgccatcaat catgccgtca atgctcagtt
    34141 gcgcctgcgc aatccgctgt ttgtcttcgc catcttttgt ttcgacgtaa tactcgatgg
    34201 tgtggctgtt aaagccatcc gccagggtga cgtaaatgcg aaaatgttca aaaaggacga
    34261 aacagagttc tttgtccggc gcgcagctca tggcgtgatg caggcgcgct ttcgataacg
    34321 tgatcccctg aaccagcgaa ttgcagtaga tgtgccactg gtcctgtagg cgacgatgcc
    34381 gctgtgcgat gtaatcggct ttctcgctta tttcccaaat agtcattgtc aggttacccg
    34441 tttaacagag atgccagctt taagcatttt ctgtgccaac ttttaatttg ttgttattaa
    34501 agagattttt aagctaaaga tgaatttcgt cgccgtgtcg acgtgtcatt tcgacatcat
    34561 cgacattatt caccgcaggg ataatcaaca ctggcacaat tattgcttgt agctggcaat
    34621 agttaatggg aggcgatatg cacgaaataa ccctctgcca acgggcactg gaattgatcg
    34681 aacagcaggc cgcaaaacac ggcgcaaaac gcgtaactgg ggtctggctc aaaattggcg
    34741 cattttcttg tgtcgaaacc agctctcttg ccttttgttt tgatctggtt tgccgcggca
    34801 gcgtggcgga aggttgtaaa ctgcacctcg aagaacaaga ggccgaatgc tggtgtgaaa
    34861 catgccaaca gtatgtgacg ctactgaccc agcgcgtccg ccgctgtcca cagtgtcatg
    34921 gtgacatgct gcagattgtg gcagacgacg gtttacagat tcggcggata gaaatagacc
    34981 aggagtgagc gatgtgtaca acatgcggtt gcggtgaagg caacctgtat atcgagggtg
    35041 atgaacataa ccctcattcc gcgtttcgta gcgcgccatt tgccccggcg gcacgcccga
    35101 agatgaaaat caccggcatt aaagcgcctg aatttacccc cagccagact gaagaaggcg
    35161 acctgcatta cggtcatggc gaagcgggca ctcacgcacc gggcatgagc cagcgtcgga
    35221 tgctggaagt cgaaattgac gtgctggaca aaaataaccg tctggctgaa cgcaaccgcg
    35281 cgcgctttgc tgcccgcaag caactggtgc tcaacctggt ttccagccca ggttccggta
    35341 aaaccaccct gctgacggaa accttaatgc gcctgaaaga cagcgttccg tgcgcggtta
    35401 ttgaaggtga ccagcaaacc gtgaacgatg ccgcacgcat tcgcgctacc ggcacaccag
    35461 cgattcaggt gaacaccggt aaaggctgcc atcttgacgc acagatgatt gccgacgccg
    35521 caccgcgtct gccactggac gataacggta ttctgtttat cgaaaacgtt ggcaacctcg
    35581 tatgcccggc cagcttcgat ctcggtgaaa aacacaaagt ggcggtgctt tccgttaccg
    35641 aaggtgaaga caaaccgctg aaatatccgc atatgtttgc cgccgcctcg ctgatgctgc
    35701 tcaacaaagt tgacctgttg ccgtatctca actttgacgt tgagaagtgc ctcgcctgcg
    35761 cccgcgaagt caatccagaa attgaaatca tccttatttc cgccaccagc ggtgaaggga
    35821 tggaccagtg gctgaactgg ctggagacac agcgatgtgc ataggcgttc ccggccagat
    35881 ccgcaccatt gacggcaacc aggcaaaagt cgacgtctgc ggcattcagc gcgatgtcga
    35941 tttaacgtta gtcggcagct gcgatgaaaa cggtcagccg cgcgtgggcc agtgggtact
    36001 ggtacacgtt ggctttgcca tgagcgtaat taatgaagcc gaagcacgcg acactctcga
    36061 cgccttacaa aacatgtttg acgttgagcc ggatgtcggc gcgctgttgt atggcgagga
    36121 aaaataatgc gttttgttga tgaatatcgc gcgccggaac aggtgatgca gttaattgag
    36181 catctgcgcg aacgtgcttc acatctctct tacaccgccg aacgccctct gcggattatg
    36241 gaagtgtgcg gtggtcatac ccacgctatt tttaaattcg gcctcgacca gttactgcct
    36301 gaaaacgttg agtttatcca cggtccgggt tgcccggtgt gcgtactgcc gatgggtaga
    36361 atcgacacct gcgtggagat tgccagccat ccggaagtca tcttctgtac ctttggcgac
    36421 gccatgcgcg taccagggaa acagggatcg ctgttgcagg caaaagcacg cggtgccgat
    36481 gtgcgcatcg tttactcgcc gatggatgcg ttgaaactgg cgcaggagaa tccaacccgc
    36541 aaagtggtgt tcttcggctt aggttttgaa accaccatgc cgaccaccgc catcactctg
    36601 caacaggcga aagcgcgtga tgtgcagaat ttttacttct tctgccagca cattacgctt
    36661 atcccgacgc tgcgcagttt gctggaacag ccggataacg gtatcgacgc gttcctcgcg
    36721 ccgggccacg ttagtatggt tatcggcact gatgcctata attttatcgc cagcgatttt
    36781 catcgtccgc tggtggtcgc tggtttcgaa ccccttgatc tactgcaagg cgtggtcatg
    36841 ctggtggagc agaaaatagc ggcccacagc aaggtagaga atcagtatcg tcgggtggta
    36901 ccggatgccg gtaacctgct ggcgcaacag gcgattgccg atgtgttctg tgtcaacggc
    36961 gacagcgaat ggcgcggctt aggcgtgatt gaatcttctg gcgtgcacct gacgccggat
    37021 tatcaacgat tcgatgccga agcacatttc cgcccggcac cgcagcaggt ctgcgatgac
    37081 ccgcgcgcgc gttgtggtga ggtattaacg ggcaaatgta agccgcatca atgcccgctg
    37141 tttggtaaca cctgtaatcc tcaaaccgca tttggtgcac tgatggtctc ctccgaagga
    37201 gcgtgcgccg cgtggtatca gtatcgtcag caggagagtg aagcgtgaat aatatccaac
    37261 tcgcccacgg cagcggcggc caggcgatgc agcaattaat caacagcctg tttatggaag
    37321 cctttgccaa cccgtggctg gcagagcagg aagatcaggc acgtcttgat ctggcgcagc
    37381 tggtagcaga aggcgatcgt ctggcgttct ccaccgacag ttacgtcatt gacccgctgt
    37441 tctttcctgg cggtaatatc ggcaagctgg cgatttgcgg cactgcgaat gacgttgcgg
    37501 tcagtggcgc tattccgcgc tatctctcct gtggctttat cctcgaagaa ggattgccga
    37561 tggagacact gaaagccgta gtgaccagca tggcagaaac cgcccgcacg gcaggcattg
    37621 ccatcgttac tggcgatact aaagtggtgc agcgcggcgc ggcagataaa ctgtttatca
    37681 acaccgctgg catgggcgca attccggcga atattcactg gggcgcacag acgctaaccg
    37741 caggcgatgt attactggtt agcggcacgc tcggcgacca cggggcgact atccttaacc
    37801 tgcgtgagca gctggggctg gatggcgaac tggtcagcga ctgcgcggta ctgacgccgc
    37861 ttattcagac gctgcgtgac attcccggcg tgaaagcgct gcgtgatgcc acccgtggtg
    37921 gtgtgaacgc ggtagttcat gagttcgcgg cagcctgcgg ttgtggtatt gaaatttcag
    37981 aagcggcact gccggttaaa cctgccgtgc gcggcgtttg cgaactgctg ggactggacg
    38041 cccttaactt tgccaacgaa ggcaaactgg tgatcgccgt tgaacgcaac gcggcagagc
    38101 aagtgctggc agcgttacat tcccatccac tgggaaaaga cgcggcgctg attggtgaag
    38161 tggtggaacg taaaggtgtt cgtcttgccg gtctgtatgg cgtgaaacga accctcgatt
    38221 taccacacgc cgaaccgctt ccgcgtatat gctaataaaa ttctaaatct cctatagtta
    38281 gtcaatgacc ttttgcaccg ctttgcggtg ctttcctgga agaacaaaat gtcatataca
    38341 ccgatgagtg atctcggaca gcaagggttg ttcgacatca ctcggacact attgcagcag
    38401 cccgatctgg cctcgctgtg tgaggctctt tcgcaactgg taaagcgttc tgcgctcgcc
    38461 gacaacgcgg ctattgtgtt gtggcaagcg cagactcaac gtgcgtctta ttacgcatcg
    38521 cgtgaaaaag acacccccat taaatatgaa gacgaaactg ttctggcaca cggtccggta
    38581 cgccgcattt tgtcgcgccc tgatacgctg cattgcagtt acgaagaatt ttgtgaaacc
    38641 tggccgcagc tggtcgcagg tgggctatac cccaaatttg gtcactattg cctgatgcca
    38701 ctggcggcgg aagggcatat ttttggtggc tgtgaattta ttcgttatga cgatcgtccc
    38761 tggagcgaaa aagagttcaa tcgtctgcaa acatttacgc agatcgtttc tgtcgtcacc
    38821 gaacaaatcc agagccgcgt cgtcaacaat gtcgactatg agttgttatg ccgggagcgc
    38881 gataacttcc gcatcctggt tgccattacc aacgcggtgc tttcccgcct ggatatggac
    38941 gaactggtca gcgaagtcgc caaagaaatc cattactatt tcgacattga cgatatcagc
    39001 attgtcttac gcagccaccg taaaaacaaa ctcaacatct actccactca ctatcttgat
    39061 aaacaacatc ccgcccacga acagagcgaa gtcgatgaag ccggaaccct caccgaacgc
    39121 gtgttcaaaa gtaaagagat gctgctgatc aatctccacg agcgggacga tttagccccc
    39181 tatgaacgca tgttgttcga cacctggggc aaccagattc aaaccttgtg cctgttaccg
    39241 ctgatgtctg gcgacaccat gctgggcgtg ctgaaactgg cgcaatgcga agagaaagtg
    39301 tttaccacta ccaatctgaa tttgctgcgc cagattgccg aacgtgtggc aatcgctgtc
    39361 gataacgccc tcgcctatca ggaaatccat cgtctgaaag aacggctggt tgatgaaaac
    39421 ctcgccctga ccgagcagct caacaatgtt gatagtgaat ttggcgagat tattggccgc
    39481 agcgaagcca tgtacagcgt gcttaaacaa gttgaaatgg tggcgcaaag tgacagtacc
    39541 gtgctgatcc tcggtgaaac tggcacgggt aaagagctga ttgcccgcgc gatccataat
    39601 ctcagtgggc gtaataatcg ccgcatggtc aaaatgaact gcgcggcgat gcctgccgga
    39661 ttgctggaaa gcgatctgtt tggtcatgag cgtggggctt ttaccggtgc cagcgcccag
    39721 cgcattggtc gttttgaact ggcggataaa agctctctgt tcctcgacga agtgggcgat
    39781 atgccactgg agttacagcc gaagttgctg cgtgtattgc aggaacagga gtttgaacgt
    39841 ctcggcagca acaaaatcat tcagacggac gtacgtttaa ttgccgcgac taaccgcgat
    39901 ctgaaaaaaa tggtcgccga ccgagagttc cgtagcgatc tctattaccg cctgaacgta
    39961 ttcccgattc acctgccgcc actacgcgag cgtccggaag atattccgct gctggcgaaa
    40021 gcctttacct tcaaaattgc ccgtcgtctg gggcgcaata tcgacagcat tcctgccgag
    40081 acgctgcgca ccttgagcaa catggagtgg ccgggtaacg tacgcgaact ggaaaacgtc
    40141 attgagcgcg cggtattgct aacacgcggt aacgtgctac agctgtcatt gccagatatt
    40201 gttttaccgg aacctgaaac gccgcctgcc gcaacggttg tcgcccagga gggcgaagat
    40261 gaatatcagt tgattgtgcg cgtgctgaaa gaaaccaacg gcgtggttgc cgggcctaaa
    40321 ggcgctgcgc aacgtctggg gctgaaacgc acgaccctgc tgtcacggat gaagcggctg
    40381 ggaattgata aatcggcatt gatttaactg caaattgccg gacagatctg cctgtccggc
    40441 atactattca cgaggttttt tcggacgata tttttccggc agttctggca ccggacgctt
    40501 gtcatcgatg agatgacgca cggttaagat cggatgacgc cacagcattc tcggcccggc
    40561 ccaacgcata atctgtttca tctcttcacg ctttgcaggc tggtaacagt gcaccggaca
    40621 ctgcttacag gctggtttct cttcgccgaa cacacattta tccagccgct tttgcgcgta
    40681 aacaaacaac gcctcgtaat gctccggctc cgctgacgcc tgcgggcatt tcgcttgata
    40741 aagatcgatc atttttttaa tcgtcagttt ttcacgagag atacgcttgc cggacatgct
    40801 gcctccatac tcttaaggtg tatttatatt acaacttaat ttgaaagggg actatggctc
    40861 tgaaggagga ggtttattca accgacgcaa ttttgcgtac ttgcttcata agcatcacgc
    40921 aaaagctgca aaacagcatc tttcccggaa ccagcatcaa gaactcgcag ttcgcttctt
    40981 cccctgaaat gattaactcc ggtatcatgt gcgccttatg tgattacaac gaaaataaaa
    41041 accatcacac cccatttaat atcagggaac cggacataac cccatgagtg caatagaaaa
    41101 tttcgacgcc catacgccca tgatgcagca gtatctcagg ctgaaagccc agcatcccga
    41161 gatcctgctg ttttaccgga tgggtgattt ttatgaactg ttttatgacg acgcaaaacg
    41221 cgcgtcgcaa ctgctggata tttcactgac caaacgcggt gcttcggcgg gagagccgat
    41281 cccgatggcg gggattccct accatgcggt ggaaaactat ctcgccaaac tggtgaatca
    41341 gggagagtcc gttgccatct gcgaacaaat tggcgatccg gcgaccagca aaggtccggt
    41401 tgagcgcaaa gttgtgcgta tcgttacgcc aggcaccatc agcgatgaag ccctgttaca
    41461 ggagcgtcag gacaacctgc tggcggctat ctggcaggac agcaaaggtt tcggctacgc
    41521 gacgctggat atcagctccg ggcgttttcg cctgagcgaa ccggccgacc gcgaaacgat
    41581 ggcggcagag ctgcaacgca ctaatccggc ggaactgctg tatgcagaag atttcgccga
    41641 gatgtcgctg attgaaggcc gtcgcggcct gcgccgtcgc ccgctgtggg agtttgaaat
    41701 cgacaccgcg cgccagcagt tgaatctgca atttggcacc cgcgatctgg tcggttttgg
    41761 cgtcgagaac gcgccgcgcg gactttgtgc tgccggttgt ctgttgcagt atgcgaaaga
    41821 tacccaacgc acgaccctgc cgcatattcg ttctatcacc atggaacgtg agcaggacag
    41881 catcattatg gatgccgcga cacgtcgtaa cctggaaatt actcagaacc tggcgggcgg
    41941 tgcggaaaat acgctggctt ctgtgctcga ctgcaccgtc acgccgatgg gtagtcgtat
    42001 gctgaaacgc tggctgcata tgccagtgcg cgatacccgc gtgttgcttg agcgccagca
    42061 aactattggc gcattgcagg atttcaccgc cgagttgcag ccggtactgc gtcaggtcgg
    42121 cgacctggaa cgtattctgg cacgtctggc tttacgaact gctcgcccac gcgatctggc
    42181 ccgtatgcgc cacgctttcc agcaactgcc ggagctgcgt gcgcagttag aaactgtcga
    42241 tagtgcaccg gtacaggcgc tacgtgagaa gatgggcgag tttgccgagc tgcgcgatct
    42301 gctggagcga gcaatcatcg acacaccgcc agtgctggta cgcgacggtg gtgttatcgc
    42361 atcgggctat aacgaagagc tggatgagtg gcgcgcgctg gctgacggcg cgaccgatta
    42421 tctggagcgt ctggaagtcc gcgagcgtga acgtaccggc ctggacacgc tgaaagttgg
    42481 ctttaatgcg gtgcacggct actacattca aatcagccgt gggcaaagcc atctggcacc
    42541 catcaactac atgcgtcgcc agacgctgaa aaacgccgag cgctacatca ttccagagct
    42601 aaaagagtac gaagataaag ttctcacctc aaaaggcaaa gcactggcac tggaaaaaca
    42661 gctttatgaa gagctgttcg acctgctgtt gccgcatctg gaagcgttgc aacagagcgc
    42721 gagcgcgctg gcggaactcg acgtgctggt taacctggcg gaacgggcct ataccctgaa
    42781 ctacacctgc ccgaccttca ttgataaacc gggcattcgc attaccgaag gtcgccatcc
    42841 ggtagttgaa caagtactga atgagccatt tatcgctaac ccgctgaatc tgtcgccgca
    42901 gcgccgtatg ttgattatta ccggcccgaa catgggcggt aaaagtacct atatgcgcca
    42961 gaccgcactg attgcgctga tggcctacat cggcagctat gtaccggcac aaaaagtcga
    43021 gattggacct atcgatcgca tctttacccg cgtaggcgcg gcagatgacc tggcttccgg
    43081 gcgctcaacc tttatggtgg agatgactga aaccgccaat attttacata acgccaccga
    43141 atacagtctg gtgttaatgg atgagatcgg gcgtggaacg tccacctacg atggtctgtc
    43201 gctggcgtgg gcgtgcgcag aaaatctggc gaataagatt aaggcattga cgctgtttgc
    43261 cacccactat ttcgagctga cccagctacc ggagaaaatg gaaggcgtcg ccaacgtgca
    43321 tctcgatgcg ctggagcacg gagacaccat tgcctttatg catagcgtgc aggatggcgc
    43381 ggcgagcaaa agctacggcc tggcggttgc agctctggca ggcgtgccaa aagaggtgat
    43441 taagcgcgca cggcaaaaac tgcgtgagct ggaaagcatt tcgccgaacg ccgccgctac
    43501 gcaagtggat ggtacgcaaa tgtctttgct gtcagtgcca gaagaaactt cgcctgcggt
    43561 cgaagcactg gaaaatctcg acccggattc actgactccg cgtcaggcgc tggagtggat
    43621 ttatcgcttg aagagtctgg tgtaataaca attcccgata gtcttttgct atcgggaata
    43681 ttaacgacaa ctgacgaatc aaataaaaat accctgtata ataggaaagc ttattttaca
    43741 gggtaaaacc atgccatcta cacgctatca aaaaatcaat acccatcact atcgccatat
    43801 atgggtcgtt ggtgatattc atggtgaata tcagttatta caatcccgct tacatcaact
    43861 ctcttttttc cccgaaaccg acttacttat ttctgtcggc gataatattg atcgtgggcc
    43921 ggagagtctt gacgtcctgc gcctgctaaa ccagccctgg tttatctccg ttaaaggcaa
    43981 ccacgaagca atggcgctgg atgcgttcgc gaccggcgat ggcaatatgt ggcttgccag
    44041 cggtggtgac tggtttttcg atttaaatga ttcagagcaa aaagaagcta cagatctgtt
    44101 gctgaaattc catcaccttc cacatattat tgaaatcact aacgacaaca taaaatatgt
    44161 catcgcacat gcagattatc cggggagtga atatctcttt ggtaaagaaa tagcggagag
    44221 cgaattactc tggcctgttg atcgtgtgca gaaatcgctt aatggcgagt tacaacaaat
    44281 aaacggcgct gattatttta tatttggaca tatgatgttt gataacattc agacgttcgc
    44341 taaccagatt tatattgata ccggcacgcc gaaaagcggg cggctgtcat tttataaaat
    44401 aaagtagtct catgcttctt ctgtgaagca tgagtaacca aatgttattg caggccatta
    44461 ttcatttttc gctaccagca aagagagatc ctgcttcgcc agcgcgcgac tggcactctc
    44521 cggcaaaccg tcgtctgtaa taatctgatc aaactcgctt aatggtaacg ccagccatgt
    44581 cgccacctga ccatatttcg tcgcatcaca gaccaaaact cgctggcggc tggcactggc
    44641 aatcgcccgt ttcaccgtga ctttatcttc cgctggcgta gaaatccccc gcacactcca
    44701 tgacgatgca gaaataaaag cctgatcaat catcaggctg cgcagcatgg tcgcagcggc
    44761 ttccccgaca caggaacggt tttcccgaca cactgcaccg ccagtgtgaa taattgtgca
    44821 attactgttg tcgagcaagt agttcgcaat aacgaaatcg tttgtgacca cagtcagtga
    44881 ctccatgtga atcagatgct gtgctatcgc taacgtggtc gttcccgcat ccagatagat
    44941 acaacttccc ggctgaacaa gacttgccgc cagcttgcca atagccgctt tttgcgtcat
    45001 tgccagcgca gtttttacct gatgagaagg ttcatgcgcc acgcgtcccg gagactggac
    45061 gcctccggac accagcacaa cggctccctg ctgctccagt ttttgtaaat cccgacgaat
    45121 ggtcatatgt gacacattca ttctgtccgt tagttcagca atactgacaa tgcctttttc
    45181 agctaccatc tcaaggatga tttggcgacg ctctacgggt atcaactttt gctccttcct
    45241 ttgtcctgct gacattctac gctatttgcc tgcgaaacgt gcgcggcgca actaacgctt
    45301 agttcacata aaataacaca caatgttaat ttatgtgaat cagctcacca tatcgttatc
    45361 ttccagcgct tatattcaca atatcaaaca aaatatcact taaattaaca aggggagcag
    45421 atgaaaacgg gatctgagtt tcatgtcggt atcgttggct tagggtcaat gggaatggga
    45481 gcagcactgt catgtgtccg cgcaggtctt tctacctggg gcgcagacct gaacagcaat
    45541 gcctgcgcta cgttgaaaga ggcaggtgct tgcggggttt ctgataacgc cgcgacgttt
    45601 gccgaaaaac tggacgctct gctggtgctg gtggtcaatg cggcccaggt taaacaggtg
    45661 ctgtttggtg aaacaggcgt tgcacaacat ctgaaacccg gtacggcagt aatggtttct
    45721 tccactatcg ctagtgctga tgcgcaagaa attgctaccg ctctggctgg attcgatctg
    45781 gaaatgctgg atgcgccagt ttctggtggt gcagtaaaag ccgctaacgg tgaaatgact
    45841 gtcatggcct ccggtagcga tattgccttt gaacgactgg cacccgtgct ggaagccgtt
    45901 gccggaaaag tttatcgcat aggtgcagaa ccgggactag gttcgaccgt aaaaattatt
    45961 caccagttgt tagcgggcgt acatattgct gccggagccg aagcgatggc acttgcagcc
    46021 cgtgcgggga tcccgctgga tgtgatgtat gacgtcgtga ccaatgccgc cggaaattcc
    46081 tggatgttcg aaaaccggat gcgtcatgtg gtggatggcg attacacccc gcattcagcc
    46141 gtcgatattt ttgttaagga tcttggtctg gttgccgata cagccaaagc cctgcacttc
    46201 ccgctgccat tggcctcaac agcattgaat atgttcacca gcgccagtaa cgcaggttac
    46261 gggaaagaag acgatagcgc agttatcaag attttctctg gcatcactct accgggagcg
    46321 aaatcatgat caagattggc gttatcgccg atgattttac cggcgcgacg gatatcgcca
    46381 gttttctggt ggaaaacggt ctaccaacgg tacaaattaa cggtgttcca acaggtaaaa
    46441 tgccggaagc aatcgacgca ctggtgatca gcctgaaaac gcgctcctgt ccggtggttg
    46501 aagccacaca gcaatcgctg gcggctctga gctggttgca acagcaaggt tgcaaacaga
    46561 tctatttcaa atactgctct actttcgaca gtacggcgaa aggtaatatc ggcccggtta
    46621 ccgatgcatt aatggatgct ctcgacacgc cgtttacggt cttctctccg gccctgccgg
    46681 tcaacggacg tacggtttat caggggtatt tgttcgtaat gaatcaactg ctggccgaat
    46741 ccgggatgcg ccatcatccg gtaaatccca tgaccgacag ctatcttccc cgtctggttg
    46801 aagcgcaatc cacagggcgc tgcggcgtcg tttcggcaca tgttttcgaa caaggtgtgg
    46861 atgccgttcg tcaagagctg gctcgcttac agcaagaggg ctaccgctac gcggtgcttg
    46921 atgcgctgac cgaacaccat ctggaaattc agggagaagc cttgcgcgat gccccactgg
    46981 taacgggcgg ttctggtctg gcgattggcc tggcccggca gtgggcgcaa gaaaacggta
    47041 accaggctcg cgaagcaggg catccgctcg ctgggcgcgg cgtagtgctc tccggttcat
    47101 gctctcaaat gaccaaccgc caggtggcac attaccgtca aattgcacca gcccgtgaag
    47161 ttgatgtggc acgctgcctc tcaaccgaaa ctctggccgc ttatgcacac gaactggcag
    47221 agtgggttct gggccaggaa agtgtacttg ctccactggt ttttgccacc gccagcactg
    47281 acgcattggc agcaattcaa cagcaatacg gtgcacaaaa agccagtcag gcagtagaaa
    47341 ctctattttc tcaactagcg gcgcggttag cagcggaagg cgtgacacgc tttattgtcg
    47401 caggcggtga gacctccggc gtagtcacac agagcctggg cataaaaggg tttcatattg
    47461 gcccaaccat ttcccccggc gtgccgtggg taaacgcact ggataagcct gtctcactcg
    47521 cccttaaatc tggcaacttc ggtgatgaag cctttttttc acgagcccaa agagagtttt
    47581 tatcatgagc gatttcgcaa aagtagagca gtctttgcga gaggagatga cgcggattgc
    47641 cagttcattc tttcagcgcg gctatgcaac cggttcggct ggcaatctgt cgctgctttt
    47701 acctgacggg aatttactgg cgacaccgac aggttcatgc ctgggcaatc tcgatccgca
    47761 gcggctttcc aaagtcgccg cggatggcga atggttaagt ggtgacaaac cctcgaaaga
    47821 ggtgctcttt catctggcgc tgtatcgcaa caatccgcgc tgtaaagcgg tggtgcattt
    47881 gcacagcaca tggtcgacgg cgctttcctg cctgcaaggg ctggacagca gcaacgttat
    47941 tcgtccgttc acaccatacg tggtgatgcg gatgggaaat gtcccgctgg tgccttatta
    48001 ccgaccgggc gataaacgca tcgcacagga tctggcggaa ctggcagcag acaatcaggc
    48061 ttttttactg gcaaatcatg gcccagtggt ttgcggtgaa agcctgcaag aagccgccaa
    48121 caatatggaa gagctggagg aaacggcaaa gctgattttt attctcggtg accgcccgat
    48181 ccgttatctg accgcaggtg aaattgcgga attaaggagt taatgcaatg cctcgttttg
    48241 cagctaattt atccatgatg ttcaccgaag tgccttttat tgaacgcttc gccgcagcgc
    48301 gaaaagccgg gttcgatgct gtggaatttc tgtttcccta tgactactcc accctgcaaa
    48361 tccaaaagca actggagcaa aaccatctga cactggcgct gttcaatacc gctcccggag
    48421 atattaatgc gggggaatgg ggattatccg cccttcccgg acgtgagcac gaagcacacg
    48481 cagacatcga cctggcactg gaatatgcat tggcgctcaa ttgtgaacaa gtccatgtga
    48541 tggcaggcgt cgtgcccgct ggcgaagacg ctgaacggta ccgggcagta tttatcgata
    48601 atctccgcta cgctgccgac cgctttgcgc tacacggcaa gcgaatatta gttgaagcac
    48661 tcagccccga cgttaagcct cattatctct tctccagcca gtatcaggca ctggctattg
    48721 ttgaagaggt tgcgcgagat aacgtgttta ttcaactaga tacttttcat gcgcaaaaag
    48781 tagatggcaa cctgacgcat ttaatccgcg actacgctgg gaaatatgcg cacgtacaaa
    48841 ttgccggact acctgatcga catgaaccgg acgatggaga aatcaactat ccgtggctgt
    48901 tccgcctgtt cgatgaggtg ggatatcagg gctggatcgg ttgtgaatat aaacctcgtg
    48961 gcctcaccga agaagggctt ggctggtttg acgcctggcg ctaattcgtt aaatccctaa
    49021 ttacaacgta cccatacatc cccccatcta cggatggggt aggttaacta taactttcag
    49081 acagggtttc ccatgtccac aattacattg ttatgcattg cgttagctgg cgtaatcatg
    49141 ctgttgctgc tggtcatcaa ggcaaaggta caaccattcg ttgctctgct cctcgtcagc
    49201 ctgttagtcg cacttgcggc gggtataccg gcgggcgaag tgggtaaagt gatgatcgcc
    49261 gggatgggcg gtgtgcttgg ttccgtcact attattattg gtctgggcgc tatgctgggg
    49321 cgtatgatcg aacactctgg tggtgcagag tcactggcta attatttcag tcgcaagtta
    49381 ggtgacaaac gaactatcgc tgcgctgact ctggcagcgt tcttcctcgg tattcccgtc
    49441 ttctttgatg tcggctttat tattcttgcg ccaatcattt acggttttgc caaggttgcc
    49501 aaaatatcgc cactcaaatt tggcctgcct gtcgctggaa tcatgctaac tgttcatgtg
    49561 gcggtaccgc cgcatccagg ccctgtcgcc gcagcggggt tactccacgc agacatcggc
    49621 tggctaacca tcatcggtat tgcgatttct attcccgtag ggattgttgg ctactttgca
    49681 gcgaaaataa tcaatacgcg ccaatatgcg atgtcggtgg aagtactgga acagatgcaa
    49741 ctggctccgg ccagtgagga aggcgcaaca aaattaagcg ataaaataaa tccaccgggc
    49801 gtcgcgctgg tcacctcgct aattgttatt cctatcgcga ttatcatggc gggtacggtt
    49861 tccgcaacac tgatgccgcc ttcgcatccc ctgcttggta cgctacagct gatcggctca
    49921 ccaatggtag ctctaatgat tgcgctggtg ctggcattct ggttattggc tttacgtcgc
    49981 ggctggagct tacaacacac cagcgacatt atgggctcag cccttcccac tgcggcggta
    50041 gtgattttgg ttactggtgc tggaggggta tttggcaaag tgctggtgga atcgggcgtc
    50101 ggcaaagccc ttgccaatat gctacaaatg attgacctgc ctctgttacc agccgcattt
    50161 attatttcac tggctctgcg tgcatcgcag gggtcagcaa ccgtagcaat cctgacaacc
    50221 ggcgggttac tctcagaagc ggtgatggga ttgaatccga ttcagtgcgt attggtgacg
    50281 ctggcagcct gctttggtgg gcttggcgcg tcacatatta atgactcagg gttctggatt
    50341 gtgaccaaat atctggggtt gtcggtagca gacggtctga aaacctggac agtgttaacg
    50401 accattctcg gttttaccgg attcttaatt acctggtgcg tatgggcggt aatttgacct
    50461 taataaaaag gtccgatggg catcggacct tttatttaac gcttaccttc caccagcagc
    50521 ggcgggatgc tcggcaccat tggcgcgtca tcaatatctt tctgcgtcat gcggaacgct
    50581 tcgggataat gttcgcggct ggtacggcgc agcgattcgg tatcgcgcca ggtataaagg
    50641 caatgctggc actgatatac cgtccagaca tctttcaccg gcgatttcgc catcacttca
    50701 atctgttcat cggcacaacg tggacaaatc atcttcttct ccttatttac gtgcagccag
    50761 catagcggtc agtttttcag cccaggcttt ggtttccggt aaatccacca ccggttggct
    50821 gtagtgacca cggttgtccg gggcgacagg cgtagtggcg tcgataatca gcttgtcggt
    50881 gatccccgca gggcttgagc ctggatcgag ttccagcacg gacatattcg gcaactgcac
    50941 caaatcccct gccgggttca ctttcgagga gagcgcccac atcacctgcg gcaggttgaa
    51001 cgggtcaacg tcttcatcga ccataatcac catcttcacg tagcccagac catgcggcgt
    51061 ggtcattgcg cgcaggccca ccgcgcgggc aaagccgccg tagcgttttt tggtggagat
    51121 aatcgccagc aggccatggg tgtacatggc gtttaccgcc tgcacttcag ggaactcggc
    51181 tttcagctgc tgatacagcg gcacgcaggt ggctggcccc atcaggtagt cgatttcggt
    51241 ccacggcata ccgaggtaca gcgattcgaa aatcggcctg gtgcggtaag agactttatc
    51301 gatgcgcacc acggtcatgt tacgcccgcc ggagtagtgc ccggtaaact caccgaacgg
    51361 cccttcgatt tcgcgtttac ggctttcgat gaccccttcg aggatcactt ctgaacccca
    51421 cggcacatca aaaccggtca acggggcggt ggcgatcggg tacgggcttt cacgcagcgc
    51481 gcctgccatt tcgtactcgg actgatcata tttcagcggc gtggccccca tcagcgtgat
    51541 gatcggatcg ttaccgagcg tgatcgcaat cggcagatct tcaccgcgct cttctgcttt
    51601 atgcagatgc agggcgatat cgtgcatcgg caccggttgc aggccgagct tacgcttgcc
    51661 cttcacttcc atgcggtaga tgccgacgtt ctgcttgccg aagttatccg ggtcgagcgg
    51721 atcgcgggaa accacgcacg ctttgtcgag atagaaaccg ccatcgccat cgtttaaacg
    51781 aaacagcggc aggatatcga acaggttgat ctcgtcgcca tcaacggtgt tctgcgccca
    51841 ggctggattg gcgcggcgct ccggggcaat cgggaagtta tcccagcggc ggataaactc
    51901 atcaatctgc tttttaaccg gggcatttgg cggcaggccg agggaaatcg cgtggttctg
    51961 ccaggaaccg atggtgttca tcgccacgcg ggcatcggta aagccgcgaa tattatcaaa
    52021 ccacagcgcg ggcgcgccgt cgccgatacg cccggtggcg ttagctgctg ctgccagatc
    52081 cggctcggcg ttcacttctt cgctgatttt cagtaactgg ccgtggtcat caagcgcctg
    52141 taaaaagctg cgtaaatcat caaatgccat tattcattct cctgagaaaa attccgggcc
    52201 tgcggcaatc cttgccagcg cctggcgtgg ggatgttcga ggccaaattg atccagcacg
    52261 cgggctacca catggtggac aatgtcatct accgtttcgg gatggttata aaaggcaggc
    52321 atcggcggca ccatcgccac gcccatgcgt gaaagtgcga gcatattttc gagatggatg
    52381 gtgctaagcg gcatttcacg cggcaccagc accagtttgc ggccttcttt gagcacgacg
    52441 tccgccgcgc gccctaccag gccatcggcg taaccagcgc ggataccggc gagcgttttc
    52501 atactgcacg gaataacgat catgccgtcg gtacgaaaag aaccggatga gatgatcgcc
    52561 gcctgatccg ccgggttatg gctgaagtct gcgagggcag caacatcgcg agcgctgtaa
    52621 ggcgtttcca gttcaatggt ggttttcgcc cacttcgaca tcaccagatg agtctcgaca
    52681 ttcggcatct cccgcagcgc ttgcagtaat gccacaccaa gaggcgcacc ggtagcccct
    52741 gtcatcccga cgatcagttt cattgcttat cctcttaatt gttcgtatac gaacgttatt
    52801 attaacatac tccttcattt ccacagcggc aaggattgaa aagtaacaac atcttcttcg
    52861 tcactaaaaa accgctatga tagcgacaga gaattagccc ggagtttaca tggcgttacg
    52921 aaataaagcg ttccatcagt tacgacagct ttttcagcag cacacggctc gctggcagca
    52981 cgagttacct gacctgacca aaccacagta cgcggtgatg cgcgccattg ctgataaacc
    53041 tggtatcgaa caggtggcac tgatagaagc agctgtcagc accaaagcaa cgttagcaga
    53101 aatgttggca agaatggaaa atcgtggctt agtcagacgt gaacatgacc ccgctgataa
    53161 gcggcgacgc tttgtctggt tgactgccga aggcgaaaag atacttgctg cggcgatacc
    53221 cattggtgac agcgtggatg aggaattttt ggggcgtttg agtggagaag agcaggagct
    53281 gtttatgcag ctggtgcgca agatgatgaa cgcatagggg gcaaatttcc ggatgcgacg
    53341 ctatcgcgtc ttatccagac tacaaataca ccgactcacc aaccgaaaga aaaaaggcca
    53401 gcctcgcttg agactggcct ttctgacaga tgcttactta ctcgcggaac agcgcttcga
    53461 tattcagccc ctgcgtttgc aggatttcgc gcaaacggcg caggccttca acctgaatct
    53521 ggcgaacacg ttcacgggtg aggccaattt cacgacctac atcttccagt gttgccgctt
    53581 cgtaccccag caaaccgaat cgacgtgcca gcacttcacg ctgtttggcg ttcagctcga
    53641 acagccattt gacgatgctc tgcttcatat cgtcatcttg cgtggtatct tccggaccgt
    53701 tctctttttc atcggccagg atgtccagca acgctttttc ggaatcacca cccagcgggg
    53761 tgtctaccga ggtaatgcgc tcgttaagac gaagcatacg gctgacgtca tcaactggct
    53821 tatccagttg ctctgcgatc tcttccgcac ttggttcatg gtccagctta tgggacaact
    53881 cacgtgcggt tcgcaggtaa acgttcagct cctttacgat gtgaatcggc aaacgaatag
    53941 tacgggtttg gttcataatc gcccgttcaa tcgtctggcg aatccaccag gttgcgtatg
    54001 ttgagaagcg gaaaccacgt tccgggtcaa acttctctac cgcgcggatc agccccaggt
    54061 tgccctcttc gataaggtcc agcaacgcca gaccacgatt gccataacgg cgggcaattt
    54121 ttaccaccag acgcaagtta ctctcgatca tccggcggcg agaggcgaca tctccacgca
    54181 gtgcgcgacg cgcaaaataa acttcttctt cggccgttaa cagtggtgaa taaccaatct
    54241 caccaaggta aagctgagtc gcgtccaaca cacgctgtgt ggctccctgc gataacagtt
    54301 cctcttcggc caaatcgtta tcactgggtt cctcttctac taaggccttt tcgtcaaaaa
    54361 cctcaactcc gttctcatca aattccgcat cttcatttaa atcatgaact ttcagcgtat
    54421 tctgactcat aaggtggctc ctacccgtga tcccttgacg gaacattcaa gcaaaagcct
    54481 ggttccgccg atttatcgct gcggcaaata acgcagcggg tttacggatt tccccttgta
    54541 acgaatttca aaatgcaagc gtgttgaact ggttccggtg ctacccatgg ttgctatttt
    54601 ttgccccgcc ttcacttctt gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc
    54661 actcaggtaa tcatcattat gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt
    54721 accggcataa acaacgcggc catctgcggt cgcgataatt gcctgtcctt tgctgcctgc
    54781 gatatcaatc cccttgttgc ccccctcaga agcgccaaag gtttcgatca ctttgccctc
    54841 agtcggccag cgccaggtgg agataggcgt actggttgat gtactgctga cagttggctc
    54901 ggttgtgctt gctgttggta ccgttacagg cgctgtgacc gtggtcgcag ctggcttgtt
    54961 gttcggcaac attttgttag cactctgttc acccgaagat tcagaatacg taattgtcgg
    55021 ttgcgacgca acagcaacgg tggaattttg tgcaggcttg atcacaactc cttgctctgc
    55081 tgcgtcggcc tgggtaatgg catttccgcc agtgattggc gtaccggaag cattacccac
    55141 ctgcaaggtc tgaccaacgt tcagcgcgta tggtgcctga atattgttgc gctgagcaag
    55201 gtcacggaaa tcgttgccag taatccaggc gatatagaaa agtgtgtcgc cttttttcac
    55261 ggtataggta ctgccgctat aactgccttt cggaatgttc ccatactgac ggttatagac
    55321 gatgcgtccg ttttccatct gtaccggctg ctgagctact ggctgcactg gctggatttg
    55381 cggttgttga gtagcctgaa tttgtggctg ctgtaccggc tgaatttgcg gttgctgggc
    55441 tgtagacgtc gtccccattt tcggcggcgg cgtaatcaac ataccagaat tggtatttgc
    55501 aggcgcattg ccattaacgg agctgaccgg tgccggtgga tttgaagtgt cagaacagcc
    55561 tgccagccat agcgaaacca gtgacaaagc cgcaatgcgg cgaacggtga attttgggct
    55621 tcccgcgctc atttatcccc caggaaaaat tggttaataa ccagtgacat aattaccgtg
    55681 caaggcaccc tactgaacac tggaaaagat gttcacgata cgctgacctg cggcaaaata
    55741 accaggaaaa atccaggtat ttcctcacgt tttaagccag ctcacccttc actaaaggta
    55801 caaagcgcac ggcctccacg gtatcgataa taaattcgcc tccccgacga cgcacccgtt
    55861 tcaaatactg gtgctcctcc cctacgggta agacgagaat cccgccttcg tccagctgcg
    55921 tcattagcgc agttggaatt tccggcggtg ccgccgtaac aatgatagcg tcaaacggcg
    55981 cgcgtgcctg ccaaccttgc catccatcgc catgacgggt tgaaacatta tgtaaatcaa
    56041 gatttttcag gcggcgacgc gcctgccact gcaaaccttt aatccgttcg accgagcaaa
    56101 catgctggac aagatgcgcc aggattgccg tttgatatcc cgaaccagtg ccaatttcca
    56161 gcacccgcga ctgcggcgtc agctcgagta attcggtcat tcgcgccacc atatacggct
    56221 gcgaaattgt ctgcccctga cctatcggca aggcgatatt gtcccaggct ttttgttcaa
    56281 acgcttcatc aacgaatttt tcacgcggca cggcggcaag tgcattcagc acctgctcat
    56341 cctgaatacc ttgcgcacgt aattgatcca gaagtgcttg tacgcgtctg cttaccattg
    56401 cgtgccaact cccacgctgt ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt
    56461 taaatccaca tgcagcggcg tgatggagac atagccctca tctaccgcag caaaatcggt
    56521 ccccggaccg gcatcacatt taccgcccgg cgggccaatc cagtacagcg tattgccgcg
    56581 cggatcttgc tgcgggatca cctgatctgc cggatgtcgt gtaccgcagc gcgtcacgcg
    56641 aatacctttg atttgatcca agggtaaatc cggaacgtta atattaagaa tacgcccggt
    56701 gcgcaacggc tctttacaca gtgcgcgcaa aattgaacag gttaccgccg cggcagtgtc
    56761 gtaatgttta tgcccgtcaa gcgagacggc aagcgccgga aaacctaaat gacggccttc
    56821 catcgcggcg gctaccgtac cggaataaat aacatcatcc cccagattcg gcccggcgtt
    56881 aattccggac acaacaatgt ccgggcgcgg acgcatcaga gcattcacgc caagatagac
    56941 gcaatcggtc ggggttccca tttgcacagc aatatcacca ttttcaaagg taaacgtgcg
    57001 cagggaggat tccagtgtca gagaatttga agcgccgctg cggttacgat cgggggcgac
    57061 cacctgaacg tcagcaaact cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg
    57121 taccccgtca tcattactca gcaatatgcg cataatcacc tgttgtgttg ataagttccc
    57181 tgacaacgct ggttgcaaaa ctacccgccg gaagccagaa acggatctct acggtgacgt
    57241 catcccacca attccagctt aattgttgcg gatacagcag catcgctctg cgcgcggctt
    57301 caactttttc gcgcaccagt aaagcttgta attcagtttc tgcggcgaca gctgcttgtt
    57361 cgaatgccag cgcttcacgc tgagttcccc attcgccact gcccggcaat gcggcggtta
    57421 tcatcagctc tttatcgtta acgcgacgct gtaattccgc cagttcttcg gtggttgcga
    57481 caaaccagct accacgtccg gctaattgta gcgcatcgcc gtcaacaact tgattaacgt
    57541 ctgctttttt gaggcgctca gcaacaatct gattaaacaa cgcactgcgg gctgccgaca
    57601 accaaaaact ccgtttattg cgatcgcgca ccggagtatt ggtttgcgcc cagcgctgcg
    57661 ccccctgcaa gttgctaccg ccaatcccaa aacgttgggc accgaagtag ttcggtacac
    57721 cttttacgca aatatcgttc agacgttgtt caacgtcatc gcgattgctc acttcgcgca
    57781 gaaccagggt aaaggcgttt cctttcagcg cccctaaacg cagcttgcgc ttgtgccgcg
    57841 catactccag cacctggcag ccttccagtt gaaaggcgct cagatcgggc atttccttgc
    57901 ccggcacgcg agcgcataac cactgttccg taacagcatg tttgtctttt tgcccagcga
    57961 agctgacttc acgggcatga attttcagga atttcgccag tgcatccgcc acaaaacggg
    58021 tattgcagcc gtttttgagg attctaacca gaatatgctc accttcacca tcaggctcaa
    58081 agcccaaatc ttccaccacc acaaagtctt ccggattggc tttcagcagc ccggtgcctt
    58141 gcggtttacc gtggaggtaa gtgagattat caaactcaat cattttgttg ccttaatgag
    58201 tagcgccacc gcttcacagg caatcccttc cccacgtccg gtaaatccca gtttttccgt
    58261 agtagtggct ttcacgttaa catcatccat atggcagccg agatcttcgg caataaacac
    58321 gcgcatttgt ggaatgtgcg gcaacatctt cggtgcctga gcgatgatag tgacatcgac
    58381 gttgccaagg gtataaccct tcgcctgaat acgacgccag gcttcgcgta gcagctcgcg
    58441 gctatcggca cctttaaatg ccggatcggt atccgggaac agcttgccga tatcccccag
    58501 cgccgccgcg ccaagcaatg catcggtcaa cgcatggagc gccacgtcgc catcagaatg
    58561 cgccagcaat cctttttcgt aaggaatgcg tacgccacca atgataattg ggccttcacc
    58621 gccaaaggca tgtacgtcaa aaccgtgtcc aattcgcatt atgtattctc ctgatggatg
    58681 gttcgggtga ggtaaaactc ggccagtgcc aaatcttccg ggcgcgtgac tttaatgtta
    58741 tccgcacggc cttcgaccaa ctgaggatgg aatccgcaat attccagcgc cgaggcttcg
    58801 tcggtaatag tcgcgccttc atttagagcg cgcgtcagac agtcatgtaa cagctcacga
    58861 gggaaaaatt gcggcgtcag cgcgtgccat aagccgttgc gatcaacggt atgagcaatg
    58921 gcatttttgc ccggttcggc acgtttcata gtatcgcgca ctggtgcggc gaggatcccc
    58981 cccgtgcggc tggtttcgct caacgccaac aatcgcgcga ggtcatcctg atgcaaacaa
    59041 ggacgagcgg cgtcatgcac caatacccac tgcgcgtcgc cagcggcttt cagacctgcc
    59101 agcacggaat cggcacgctc atcaccgcca tctacaacgg tgatttgcgg atgattcgcc
    59161 agaggaagtt gtgcaaaacg gctatcgcca ggacttatgg caatgacgac tcgtttcacc
    59221 cggggatgcg ccagcagcgc atgcaccgag tgttcaagaa tggtttgatt accgattgag
    59281 agatattgct taggacattc cgtttgcatt cgacggccaa atccggccgc cggaaccacg
    59341 gcgcaaacat ccaaatgagt ggttgccatg ttaattcccg ggctgattta tcgattgttt
    59401 tgccccgcag actgtgcgcg cttcgacgcg tcaggcacca gacgataaaa agtttcgccc
    59461 ggcctggtca tgctgagttc attacgcgca cgctcttcga gcgcctcctg gccgccattg
    59521 agatcgtcaa tttcggcaaa aagttgatcg tttcgcgctt taagtttcgc gtttgtagct
    59581 tgctgtgccg ccacatcatc attgacgcgg gtatagtcat gtataccgtt cttaccgaac
    59641 cacagcgaat actgtagcca gaccagaata gccagcaaca gcagcgttag tttacccatc
    59701 ctgccccctg aaaaacggca tcatcatccc atgcatccga agacgactct acatcctctg
    59761 ttggggatac cgcgacaacg cgggcaaatg taccacattt gtccattgtt acgtataccc
    59821 agggcgtgca gaacataatc tcattattag ttacggtttg aattatgaac agaggagacg
    59881 ggaaagtaca aattagccca gtagccacat aaacagtgcg ccaaacataa tgcctactgt
    59941 caccagggtg aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat gcagcgcaat
    60001 ccccaccact accgcgacgg gcatcagcgc cagaaagaaa ggccaggtgt agataaagaa
    60061 gaacagcgtg ttagagccat aaatcaacat cggcatcgcc agcgcaaata accaggagat
    60121 aaaaccgacc acggcaccag gcagtgacca tgtggtttct tcatcctcag taaggctgtc
    60181 gttatttgtt agtgtaatgt tatggctatt acgcatattt gatcctgtta ctttgacgaa
    60241 ccgggcatgg aaacccggtg gtgtctcagg atctgataat atcgttctgt ctcaacagat
    60301 ctaataattg ctgtaccaaa tttgttacta attgttcacc attgagatga atttctgccg
    60361 attcaggcgc ttcgtaaacg gaatctattc ccgtaaagtt gcgcagttca ccggcacgcg
    60421 ctttcttata taagcctttt ggatcgcggg cttcgcaaat cgccagcggc gtatcgacaa
    60481 acacttcgat aaagcgccct tctcctacgc gttcgcgaac catctggcgt tcggcgcggt
    60541 gtggcgagat aaatgcggtc agcactacca gtccggcttc aaccatcaaa ttcgccactt
    60601 caccgacgcg acggatattc tctttacgat cggcatcgct aaaaccgaga tcgctgcata
    60661 atccgtggcg aacattatcg ccatccagca gatacgtact gacgccgagt ttatgtaacg
    60721 cctcctccag cgccccggcg accgttgatt taccggaccc ggagaggccg gtaaaccaca
    60781 gcactacacc acgatgaccg tggtgtagct cgcgttgttg cacagtgacc ggatggctat
    60841 gccagacgac gttttcgtca tgcagcgcca ttatttatcc cccagcaaat cgcgcgcgcc
    60901 ccagtgcgga aagtggcgac gaaccagagc attcaattcc agttcgaatg cactgaattc
    60961 agatggcgca gcagttgcct ggctaactgg ctcgtgcacc ataccggcac ccacggtcac
    61021 attgctcagg cgatcgataa aaatcagccc acccgtcacc ggattttgtt gataacgatc
    61081 taacaccagc ggctcgtcaa aagtgagatc cacgaggccg atcccattca gtggcaggtt
    61141 ttcaacttca cgctgggtaa ggttattaat atcaacctga tagcgaatgc catcaacacg
    61201 cgcgcgcgtc ttcttaccgg caattttgat gtcgtaactc tgccctggag aaagcggctg
    61261 ttccgccatc cataccacat ccaccgacgc gctctgcacc gccggtaacg cttcgtctgc
    61321 cgccagcagc agatcgccac ggctgatgtc gatctcatcc gtcagcacca gggtgatcgc
    61381 ttctccggca aaggcttctt cgcgatcacc atcaaaagtc acgatccgcg cgacgtttga
    61441 ttccacacca gagggcagca ctttgacacg ttgcccgact tccacgcgac cggatgccag
    61501 cgttccggcg taaccacgaa aatcgagatt cgggcgatta acgtactgca ccgggaagcg
    61561 cattggctga gcatccacca ctcgctggat ctccacggtt tccagcactt cgagcagtgt
    61621 cggaccgctg taccacggca tactttcact ttgcgatgcc acgttgtcgc cttccagtgc
    61681 agagagcggc acaaagcgga tatccagatt acccggcagc tgcccggcaa aggtcaaata
    61741 atcttcacga atacgggtga acgtctcttc actgtaatcc accagatcca ttttgttgat
    61801 cgccacgacc agatgtttgc tccccaacag tgtggagata aaactgtgac gacgggtttg
    61861 atcgagcacg cctttacggg catcgatcag taagatcgcc agttcacatg tcgatgcgcc
    61921 agtcgccata ttgcgggtgt actgctcgtg ccctggggtg tcggcgataa taaatttacg
    61981 cttctcggta gagaaatagc ggtaggccac gtcaatggtg atgccctgtt cgcgctcagc
    62041 ttgcaggccg tccaccagca gagccagatc cagcttttcg ccctgggtgc cgtgacgctt
    62101 actgtcgtta tgcagcgatg agagctgatc ttcgtagatt tggcgggtat cgtgcagcag
    62161 acgaccaatc agagtacttt tgccgtcatc gacgctacca caggtcagaa aacgcagcag
    62221 gcttttatgt tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg cgatttgttg
    62281 tgcaagtgcg gtgttcatct taaaaatacc cctgacgttt tttcagctcc atagaccccg
    62341 cctggtcgcg gtcaatcacg cggccctgac gttcactggt ggtggaaacc agcatctctt
    62401 cgatgatttc cggcagtgtt tgtgcatttg actccaccgc accggtcagc ggccagcagc
    62461 ccagcgtacg gaaacgcacc atccgttttt taatcacttc gcccggttgc aggtcgatac
    62521 ggttgtcatc aatcatcatc aacataccgt cgcgttccag aaccggacgt tccgcagcga
    62581 gatatagcgg aacaatgtcg atattttcca gccagatgta ttgccagata tcctgctcgg
    62641 tccagttaga gagcgggaag acgcggatgc tttcgccttt gttaatttgc ccgttgtagt
    62701 tgtgccacag ctccgggcgc tgatttttcg gatcccagcg atggaagcgg tcacggaaag
    62761 agtaaattcg ctctttagcg cgggatttct cttcgtcacg gcgcgcacca ccgaaggcgg
    62821 catcaaaacc gtatttgttc agcgcctgtt tcaggccttc agttttcata atatcggtat
    62881 gtttcgcgct gccgtgcacg aatggattaa tccccatcgc cacgccttcc gggtttttat
    62941 gcaccagcag ttcgcagccg taggctttag cagtacgatc gcggaactca tacatctcgc
    63001 ggaatttcca gccggtatcg acatgcagca acgggaaagg cagcgtacct ggataaaacg
    63061 ccttgcgcgc cagatgcagc atgacgctgg aatctttacc gatagagtag agcatcaccg
    63121 gatttgagaa ttctgccgcc acctcgcgaa taatgtggat gctttccgcc tccagttgcc
    63181 gcaggtgagt aagtcgtatt tgatccataa ccgttccttt gcaataccgc tattttcttg
    63241 ccatcagatg tttcgactat agggagcgta agagaacgaa tgaaattacc aattagaatg
    63301 agtagttcct taacggaata acgatttggc aaagctaata tcaaaaagtg cttaaggcac
    63361 cggatttcgg gcgtttagga agatttgaaa ttgttttagc gcagcggcag tttcatacta
    63421 tggcggtaaa aaaatttgca tggtatttaa ggactcacta tgttttccgc attgcgccac
    63481 cgtaccgctg ccctggcgct cggcgtatgc tttattctcc ccgtacacgc ctcgtcacct
    63541 aaacctggcg attttgctaa tactcaggca cgacatattg ctactttctt tccgggacgc
    63601 atgaccggaa ctcctgcaga aatgttatct gccgattata ttcgccaaca gtttcagcaa
    63661 atgggttatc gcagtgatat tcggacattt aatagtcggt atatttatac cgcccgcgat
    63721 aatcgcaaga gctggcataa cgtgacggga agtacggtga ttgccgctca tgaaggcaaa
    63781 gcgccgcagc agatcatcat tatggcgcat ctggatactt acgccccgct gagcgatgct
    63841 gacgccgatg ccaatctcgg cgggcttacg ttacaaggaa tggatgataa cgccgcaggt
    63901 ttaggtgtca tgctggaatt ggcagaacgc ctgaaaaata cgcctaccga gtatggtatt
    63961 cgatttgtgg cgaccagcgg cgaagaggaa gggaaattag gcgcagagaa tttactcaag
    64021 cggatgagtg acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga taacttaatt
    64081 gttggcgata aattgtattt caacagcggt gtaaaaaccc ctgaagcagt aaggaaatta
    64141 acgcgcgaca gggcgctggc aattgcgcgt agtcatggaa ttgccgcaac gaccaatccg
    64201 ggtttgaata aaaattatcc gaaaggcact gggtgttgta atgacgcaga aatattcgac
    64261 aaagcgggca ttgctgtact ttcggtggaa gcgactaact ggaatcttgg gaataaggat
    64321 ggttatcagc aacgcgcaaa aacacctgcc ttcccggcgg gaaatagctg gcatgacgta
    64381 agactggata atcaacaaca tattgataag gcacttcctg gaagaataga acgtcgctgc
    64441 cgtgatgtta tgcggataat gctaccgctg gtgaaggagc tggcgaaggc gtcttgatgg
    64501 gtttgaaaat gggagctggg agttctaccg caggggcggg gaattctaag tgatatccat
    64561 catcgcatcc agtgcgcccg gtttatcccc gctgacgcgg ggaacacaaa accaaacttc
    64621 tccataaatt ccatagccgc ggtttatccc cgctggcgcg gggaacacga gtctatcagc
    64681 gacactaccg gcaatagcga cggtttatcc ccgctggcgc ggggaacacg agagtgctga
    64741 caggtgtctc gattacctga tcggtttatc cccgctggcg cggggaacac ggtagtacgc
    64801 gcctccggac gtttttatgt cgcggtttat ccccgctggc gcggggaaca ctcttcgcgg
    64861 gtaatcaatg atgattcagt ttccggttta tccccgctgg cgcggggaac acgaacgcaa
    64921 tatcacggcg ttctgtggcc tcggcggttt atccccgctg gcgcggggaa cacgtcgggc
    64981 cgggtaagtg aacctgtcgg ttttccggtt tatccccgct ggcgcgggga acacactaag
    65041 catacataac tgtttttaaa caaatttatt ccgcatcaac aatctaccaa ctaaattaaa
    65101 acatttcctt atttttaaag aacggataac ttattgatta tcaacaggaa gaaaagaaac
    65161 caaacgtaac ccatccaaat ccaccggaat acgtctgttt tctccccagg tctgaaattc
    65221 aaaacccgac tcggtattgg tcgcccaggc catcaccaca tttccgcaac cagccagttg
    65281 ggtaatttgc tgccagatca tctcccgaat acgttttgat gtatcaccaa catacacacc
    65341 ggcacgcact tccagtagcc agattgcgag ccgtccacgt aagcgcggcg ggacattttc
    65401 tgtaacaacc acgaccatgc tcatcagccg cgtcctcgat gaccgctatc gcccaacgtt
    65461 tcaggttcgg ggatggctgg cggtaacata tccggcgcag gttgtggcgg ttcaatttca
    65521 cctgcggcaa ggacttcctc aattaacggt attaatttgc ccgttaactt agtgctacgg
    65581 aaaatatcgc gacaggctaa tctgacttct ttatcaggtt ctgcgggttg cctcgctgct
    65641 atttcaaatg cctttggcac aaccgaatca aatttaatga tatcggctat gtcataaaca
    65701 aatgaaagcg gtttgccact atggataaac ccaatagcgg gcgcatatcc cgcggctaat
    65761 actgccgctt cagatatacc gtacagacat gatgtagcag cactgatgca gcgattcaca
    65821 acatcgcctt tttcccagtc tttaggatcg tatttgcgac cattccattt cacaccatat
    65881 tgtttcgcca gtaatgcata cgtctggcga acgcgagatc cctcaattcc acgtaactgc
    65941 tccactgaac ggcgagctgg ctgtggttca cgaaaacgta attcatacat tttgcgcacc
    66001 accttcaacc gtagatcttc cgttaaagcc agctttgcct ggtagagtaa tttatctgcc
    66061 cgcgccccac cgggttgtcc ggaggagtaa acgcgaacgc ccgcttcacc gacccagacc
    66121 agcagtgttc ccaccgtggc ggccagatgc accgccgcgt gggaaactct cgttcctggt
    66181 tcgagcataa tgcaggcgac cgatcccacc ggaatatgcg ttcggatccc tgttttgtcg
    66241 atcagcacga aagcgccgtc cagtacgtcg atctgaccgt actggaggaa gatcatggag
    66301 gtgcgatctt ttaatgggat cgggctcagt ggtacaaacg tcacgcctct gctccgggtt
    66361 tgatcagcat cagaccacag ccgaacgccc ggcttttacc atacccctgg ctcaggcgtt
    66421 gcacaaataa cccaggatcg gtgaccgtaa gcatccccgt ataatccacg ctactgaact
    66481 ggatcagttg ccgggagttt tcccgccgca gttgctgttg tcggtaggca tcaaccgagg
    66541 tatccagcag tgtaaaaccg cttctctccc cctgcgctgc cagccagtcc agcgccgcct
    66601 gttgttgatg caaccagaca tcacttcctt ccgccggccc cctcacctgc cgtttcgcct
    66661 ccatcagcag atcgtggcgc ttgcccgctt tacagattgt tggattcgcc cgcaggttga
    66721 aacaaagttg ttgtccggta cacagctcgg gcgcaaatga ccggcattct atggtgaacg
    66781 tatcactttc cgccgggcgt tcctgcgaca gtacaaaaaa gcgaaacgcg ccctggagtt
    66841 cttcccgacg ataaagaaat tgcctttctt tgccgccagg gaagagatcc cacagccact
    66901 gatgcatcac atattcaccg cgatccacca gatgcagcaa ctgcgcaggc gaaagctggc
    66961 cggtatgtaa ggttattctt gagaggtaca tggttcctcc ttgctgagcc acggcccctg
    67021 attgacggtg cgttccccaa acagccactg ctgacgattt aaaggaacat cccggcggcg
    67081 taatattttg ctcgcgacca ggccgtcgtg tttcccttcc caccagcatt catcctgaag
    67141 tttcgggagt gagactttca gtttatggaa atgatcctga tactgttggt atgcattaca
    67201 taaggcatca cacgcgttgc cttcgagcag taacggcgca agaggtaatg ccagcggatg
    67261 actttttcgc cccagataaa gcggaaaaac tggatggcgt aaaccgtcct gcaactgttc
    67321 gaggctgtaa ggcgcatcgg ctgttgttgc caccgccacc atccaccagg catcggtgta
    67381 gtagtcgcgc cgggagataa tcgcgctcaa aagctcaggg tcgctcaact cttcgcgacg
    67441 gctgaaataa cgtgctttac gcacctcttt tggcatctgg accgtgtgat aatcccgtgc
    67501 ccagcgcggg ttacggctgg cgcaaaccac cagtgaatag tggcgattaa acgcgtttaa
    67561 gcgttcggta tcatcacgcc gaatccctac cccggcagcc agtagcccca gcaatgctga
    67621 gcgcgaagga agttcatggg tatgacgaac ttcacctgga gcatcgaccc cccaggacgc
    67681 catcggccca tgaagctgaa aaatcagata ttggctcata agccgcccct tacgcgcaga
    67741 taaagtccag cacgtccttc atgcttccct gtttgttcat cacgtcaaaa cttgcgcatt
    67801 cggtcttctg ttcgtagacc gtattcatgt tttcgcgaag cgttgtaata cgctgcaccg
    67861 ccacatctaa ctgacgtgtg ccattaatgg gttcatagaa agccgccgcc agagagcgtg
    67921 gttgatcggt gcctttttct gccagcgccc aggaggcgta ggcacggctg gcaaagctgt
    67981 tctgtttgcc ggttggggag actttaagtg cggcttccgt aaaggcacgt atggtctgat
    68041 tagctaacgc ttcgtcaccg ccaaggtttt cgaccagcag atctttatca atgcagatat
    68101 aggtgtagaa cagtgcagaa ccgaatccgg tttcaccaag atgcccggca ccggcatctt
    68161 cagaagcctg gcgcaaatca tcaacggcgg tgaaaaaatc atcttcgaca atcgtttcac
    68221 tgacaccaaa tgcgtgcgct acctggcagg cggcttcaac attaaactcg ggtttattcg
    68281 ccagcatacg accaaacatg gcaatatcta ccgccatacg gtctttgcgt aacaacgcga
    68341 gatcttcctc ttttggcgcg cgcttttctt ctgcaagctg atgggccagc gcttttacgg
    68401 cgtcaaattc tgccgggctg atatggacta actgttcagt ttcggcgtta gtgagcggat
    68461 cttttggttt tttgtcattt ttagctttcc caagataatc agcaattttt gccgaccatt
    68521 cgatggcttt tttatcttca atccctttct caatcaggat agttgccgcc tcacgcgcaa
    68581 tacgcccact gcgaatacca atatggcccg ccagtgcctg ttcaaaaagt gcagaagtgc
    68641 gccacgcacg tttcagactt tgcgaggaaa cgcgcagacg cgttgctcca cccagcacca
    68701 cagtttttgg cgcaccggta tcatcacggt taaggttggc agcagggtaa gcggttaaca
    68761 aatgaagctg aataaatgtt gtcatgagat agtcctttat tgattttgtt cgttatcggc
    68821 gtcgccagcc tggtaatatt ccagcgccca gcggatacgg ataaattcgg ttggccgctg
    68881 ctggcggcgg tgatgattga gcaggtcgtc gctctcctgg caccaacgga agaccccctc
    68941 tgccagagag tcaagattga cggaaccgtt taataatctg actgcgcggc gtaactggcg
    69001 cagtaattca tccggtgttt ttactgccga cagacgagta aaacgcccct ttgacattac
    69061 cgccgccagc tgcgcggcaa aaggctgccg ttcatcaata gcttttacat tggcgctaac
    69121 tgcggcgacc agcgccagtg cggtaaaacg ccactcttgt gcttcaattt tccacaaagt
    69181 gtgggtctgc atcagcagag aacgaaaccc ttctgacaag caaacatcat taaccgttgt
    69241 gctacgccgc agacttgcac gattgccgcg tttagcctgc aactcttcgc gccatttacg
    69301 cagcgtggct ttgtgctcct cttttacaat actcattcag cagcctcctg ctttttctcc
    69361 ctggcggctt tagcactttg cttctccgcc gatgttgtga aatatttctt gcgcgcggtc
    69421 ataacgcgtt tcaaatcaac aggctcatat ggattggtga atacacgctc gtcaaaatcc
    69481 tgacgcgcga ataaccaaat ttccttttgc catttgccga gtaattcgtc cgcatcctga
    69541 ccttcttcaa tttggcgcac taacctcagg aagcgatgct gagttttgtt ccagaagtcg
    69601 atatccacaa aactgaaatc accccgtgca ccttttggat cggagaacca cgcttctttc
    69661 aatgcacttc gtaacagact tagaatccgt gaagccgttt gcgcagccag acgcagtttc
    69721 ggtatctggc cttctttttt cttgagcagc agcgggaaat ggtgttcgta ccaacagcgc
    69781 gctttcatgt tgtcgaaatc atagccaaat ccccacaggc ctacttttgc ctgtttcaga
    69841 ctgctggcat taaagagttt caccaccagc gcgggaagtt ccgtattgtt ttctgattta
    69901 cccgtttcga taaggcctaa ccagtcgcgc cagattaaac cgcccggttg tggtttgacc
    69961 gagtaaaact cgccaccctc tttaagtggt atacggtaag gcgttaaggg atgctgccac
    70021 atggcataat tcgcaccgta atttttggtc gtcatcaggc tcaggagcgc gtcgctctgt
    70081 tcaccgcaaa tatcgcagtt gccgactgtc gtggtattaa aatcaatacg aatacggcgc
    70141 ggcattcccc agtaagcctg gagtttattg acctgatcat gggttaccac cgcaccggcc
    70201 agttcgctgg ttcgcgtcgg gccaagccag gggaaaacca gatcgtcaaa ttttttgggt
    70261 agcggtaagt cggcttcatc ctgcggcatc acattgggcc acagtttgcg ccacaagggc
    70321 gtttgttgat tgccttgata ttcctgtaat tcaatcagag tggtcatcgg cccaccgccg
    70381 cgcaaaccgg tgcgatagcc tttgccgcct gatggcgcat ttaactgtag ggagaacaga
    70441 gctaacgcgg aacaatgagg gcatacgtgt tcagtcacgc cacgcttaat aaagtggtct
    70501 ttattaaact tcgttgtttg agcgccggga atctcaggca gtagcgaagc gacctgaact
    70561 ttatctccct tgagcgcctc gaaatcctgc ataaatgaag gggaatctgc cccaaactgg
    70621 aaagcgtgtt ctaaggacag caatgcttcc cgtagctttt cagcctccag tccgtcttgc
    70681 cagatatcat cccaacgacc gtgatttttg ggcgcgaagc tggtttgtag tagccccagc
    70741 aaaaactgcc aggccgcccc ctggagatct gcccgcggcg cagcgatatc gacaacattt
    70801 tcatcagcca gatcgactgg tgccagcttg cctgtcgttc cgtctttaaa acgaacaggc
    70861 aaccacgggg ttgtcagaag tgaaaacgag ttcatacgca tcctctcgta aaaaagacca
    70921 tcccaggtcg gttgtccatc tttccatcct tttcggtgta tcaactattc agagagtcct
    70981 gtcgcagaca tctcaaccaa ccttgccaat caatccttcc gtagccgaat acccgcaact
    71041 ttcgtcatcg ttgactaaaa tcacgtttgc catctccgga tcttgtcgct gttcaatgca
    71101 ccacagcctg aacgcctctc cctccagtaa agaaaactca tcccgatgtt ttttccacca
    71161 gcttcggcgc actctgacaa cgctcatttc ccatgcgtga gcaccggtgg cataaggctt
    71221 caccacaccg tcaatacagg tagccagcca cagggaaaca gactcctcag ccagacgtgt
    71281 cgacagtttt tccgggaggt aatcgttgat attggcggca tagccgggcc tgaagttcag
    71341 cacaaacttt ttagccattg cgcgatcgca gtaatatttg cccacttgct cctgctcgct
    71401 gcgggcaaat ccttccggca ttaccacgtc ctcaccgtag acggattcaa taagaagccg
    71461 ggctgcgtgt ggcatctgaa tagcaccttg ctcacgcagc acacgctgcg tcagccagat
    71521 tcgcccatga tcgggataga caaatgcact gttacgcatg gcactgccaa accattcgtc
    71581 gccaggagag tcgtcccaga cgggagccag aatcagcaat tcaggggggg aacgctcgtc
    71641 ctttccgtca cgctttaact gaccattaat atcgcggata tgccgctgta atcgtcctgc
    71701 tcgctgaatc agcaaatcaa caggggccag gtcggagatc atttcgtcca ggtcaatatc
    71761 cacgctttgc tcaattactt gtgtacaaac aatgacttgc gacgctctct gtaaactaca
    71821 atatttacca aagcgtgcca gcgtttccgt ttcaattcgc tggcgatcgc taaaagcaaa
    71881 gcggctatga aaaagtgaaa ggctggaagc aggaatgacg ccgcgggcaa gcagctgacg
    71941 atgaacctta atagcgtcat cgacagaatt ccggatccag gcgatgcatt tcccctgact
    72001 taccgccgat tcgatacgcg caatacactc ttgttcacta tgaagccaac ccacgctgac
    72061 gctacgctca acatctttgc gcgtcgctac ccggtgtgag ttcacatcgg atttcgtgac
    72121 atgcgtcagc caggggtaat catccttttc caggaacgga gcttcttgct ggccctatgt
    72181 accccgcgca aaggcagcga cgagtttgtc gcgctgctgt tgagataacg tggcagaaag
    72241 caaaataacg ctgtttccgc cacgcgcctg ccgctcgatc aacccttcaa gaatgcacga
    72301 catgtaagcg tcacaggcgt ggatctcatc agccagcagg attttgttac tcagccccag
    72361 aagccgcaga ttattatgtt tgaacggcat caccgccatc atcgcctgat ccagcgtgcc
    72421 gacgccaatt tcagccagca acgccttctt gttactgtcg gcaaaccagg ccgcacatcc
    72481 ctgactgaac gtttgttcat ccggttcttc tgacccgact aaatcaccag accagagtga
    72541 ttcattaaag cggtccatta atgtgcgggc actatgtgcc agcaccaggc tggggcggga
    72601 ttctggcgaa tagaaagcaa gccaggtttt gaccagccga tcgtacatgg cattggccgt
    72661 tgccattgtt ggcagaccaa aaaacagccc ctgagctttc cccgcagcta tcaacctgtg
    72721 cgccaggata agcgccgctt ctgttttacc tgcgccagtc acatcttcca gaataaataa
    72781 ctgcggtcct tgctggctga tatccagatc cagcaccttt tgctgtaacg gtgtcggatt
    72841 ctcaataaaa ggaaacagcg tattaattcc gttgaaaggc gcgacttttg cttttagagg
    72901 aaagacggtt aaggcgtttt gagcctgaat taaagttttc tgccagtaac ctttaatatc
    72961 cattggatgt gcgacgcgtg gaaaaaatcg cgttgacgaa cccgtccagt ctgcgagcac
    73021 gacggttgca gagatatacc aggaaaggtg ttttataagt tcaatgccct catcatcatc
    73081 ccagaatgcg gggatctcta tgagcggaaa cagtggtttg atttcaagga gaaaatctcg
    73141 tgctgcagct ttgtcttcag gcagaaaatt atccaattca tccatacgat cagggggctg
    73201 accatgatgc cctgtcgtca cgggcatcca catttctatc actcgcctaa gtttgcgcgg
    73261 agagagcgaa gatgaaggaa gtaactcctg acattcactt aaataatgat tccacaacca
    73321 gtagcccagc gttgaatgag agattttttc gtaatttttt ctggcacctt cctgaatctt
    73381 gagttcaggg ggcaggtaaa gttgttgaaa agagcgggca aattttccaa tatcgtgcca
    73441 gcacagcaac caggcgaaaa attgagccgc ctgttccttg tcagaaatcc ctaattgacg
    73501 aaaataatca gccagcccga agcaatttct tttaaccatt aaatagccca tcgcggctac
    73561 atccagcgaa tgccagcaaa gaaggtgata gtcgtcgccc ccgtctttct cgccgcgtcg
    73621 ggtttttccc cagaaatcaa aa
//
LOCUS       DAZFJF010000009        69484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFJF010000009 DAZFJF010000000
VERSION     DAZFJF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.009"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..80)
                     /locus_tag="ACHKAU_000693"
     CDS             complement(<1..80)
                     /locus_tag="ACHKAU_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5529816.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
     gene            complement(263..1615)
                     /gene="pmbA"
                     /locus_tag="ACHKAU_000694"
     CDS             complement(263..1615)
                     /gene="pmbA"
                     /locus_tag="ACHKAU_000694"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO5529817.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            1709..2260
                     /gene="yjgA"
                     /locus_tag="ACHKAU_000695"
     CDS             1709..2260
                     /gene="yjgA"
                     /locus_tag="ACHKAU_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO5529818.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(2416..3789)
                     /gene="mpl"
                     /locus_tag="ACHKAU_000696"
     CDS             complement(2416..3789)
                     /gene="mpl"
                     /locus_tag="ACHKAU_000696"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO5529819.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            3965..4963
                     /gene="fbp"
                     /locus_tag="ACHKAU_000697"
     CDS             3965..4963
                     /gene="fbp"
                     /locus_tag="ACHKAU_000697"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO5529820.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(4996..5991)
                     /gene="yjfF"
                     /locus_tag="ACHKAU_000698"
     CDS             complement(4996..5991)
                     /gene="yjfF"
                     /locus_tag="ACHKAU_000698"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO5529821.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5978..7000)
                     /gene="ytfT"
                     /locus_tag="ACHKAU_000699"
     CDS             complement(5978..7000)
                     /gene="ytfT"
                     /locus_tag="ACHKAU_000699"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO5529822.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(7014..8516)
                     /gene="ytfR"
                     /locus_tag="ACHKAU_000700"
     CDS             complement(7014..8516)
                     /gene="ytfR"
                     /locus_tag="ACHKAU_000700"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO5529823.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(8656..9612)
                     /gene="ytfQ"
                     /locus_tag="ACHKAU_000701"
     CDS             complement(8656..9612)
                     /gene="ytfQ"
                     /locus_tag="ACHKAU_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO5529824.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            9922..10452
                     /gene="ppa"
                     /locus_tag="ACHKAU_000702"
     CDS             9922..10452
                     /gene="ppa"
                     /locus_tag="ACHKAU_000702"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO5529825.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(10532..10882)
                     /gene="chpB"
                     /locus_tag="ACHKAU_000703"
     CDS             complement(10532..10882)
                     /gene="chpB"
                     /locus_tag="ACHKAU_000703"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO5529826.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(10876..11127)
                     /gene="chpS"
                     /locus_tag="ACHKAU_000704"
     CDS             complement(10876..11127)
                     /gene="chpS"
                     /locus_tag="ACHKAU_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO5529827.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(11339..11680)
                     /gene="ytfP"
                     /locus_tag="ACHKAU_000705"
     CDS             complement(11339..11680)
                     /gene="ytfP"
                     /locus_tag="ACHKAU_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO5529828.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(11683..15462)
                     /gene="tamB"
                     /locus_tag="ACHKAU_000706"
     CDS             complement(11683..15462)
                     /gene="tamB"
                     /locus_tag="ACHKAU_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO5529829.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(15459..17192)
                     /gene="tamA"
                     /locus_tag="ACHKAU_000707"
     CDS             complement(15459..17192)
                     /gene="tamA"
                     /locus_tag="ACHKAU_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO5529830.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17398..18036
                     /gene="msrA"
                     /locus_tag="ACHKAU_000708"
     CDS             17398..18036
                     /gene="msrA"
                     /locus_tag="ACHKAU_000708"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO5529831.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            18359..19702
                     /gene="paeA"
                     /locus_tag="ACHKAU_000709"
     CDS             18359..19702
                     /gene="paeA"
                     /locus_tag="ACHKAU_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO5529832.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(19764..19970)
                     /gene="ytfK"
                     /locus_tag="ACHKAU_000710"
     CDS             complement(19764..19970)
                     /gene="ytfK"
                     /locus_tag="ACHKAU_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO5529833.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            20295..20852
                     /gene="ytfJ"
                     /locus_tag="ACHKAU_000711"
     CDS             20295..20852
                     /gene="ytfJ"
                     /locus_tag="ACHKAU_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5529834.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(20842..21582)
                     /gene="cysQ"
                     /locus_tag="ACHKAU_000712"
     CDS             complement(20842..21582)
                     /gene="cysQ"
                     /locus_tag="ACHKAU_000712"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO5529835.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            21772..23715
                     /gene="cpdB"
                     /locus_tag="ACHKAU_000713"
     CDS             21772..23715
                     /gene="cpdB"
                     /locus_tag="ACHKAU_000713"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO5529836.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(23844..24224)
                     /gene="ytfH"
                     /locus_tag="ACHKAU_000714"
     CDS             complement(23844..24224)
                     /gene="ytfH"
                     /locus_tag="ACHKAU_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO5529837.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            24313..25173
                     /gene="qorB"
                     /locus_tag="ACHKAU_000715"
     CDS             24313..25173
                     /gene="qorB"
                     /locus_tag="ACHKAU_000715"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5529838.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            25281..26246
                     /gene="ytfF"
                     /locus_tag="ACHKAU_000716"
     CDS             25281..26246
                     /gene="ytfF"
                     /locus_tag="ACHKAU_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5529839.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            26354..27016
                     /gene="ytfE"
                     /locus_tag="ACHKAU_000717"
     CDS             26354..27016
                     /gene="ytfE"
                     /locus_tag="ACHKAU_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO5529840.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(27263..28330)
                     /locus_tag="ACHKAU_000718"
     CDS             complement(27263..28330)
                     /locus_tag="ACHKAU_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529841.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(28435..28863)
                     /locus_tag="ACHKAU_000719"
                     /pseudo
     CDS             complement(28435..28863)
                     /locus_tag="ACHKAU_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28990..29088
                     /locus_tag="ACHKAU_000720"
                     /pseudo
     CDS             28990..29088
                     /locus_tag="ACHKAU_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29130..30533)
                     /gene="cycA"
                     /locus_tag="ACHKAU_000721"
                     /gene_synonym="dagA"
     CDS             complement(29130..30533)
                     /gene="cycA"
                     /locus_tag="ACHKAU_000721"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO5529842.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(30842..31462)
                     /gene="fklB"
                     /locus_tag="ACHKAU_000722"
     CDS             complement(30842..31462)
                     /gene="fklB"
                     /locus_tag="ACHKAU_000722"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5529843.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            31681..32319
                     /gene="ytfB"
                     /locus_tag="ACHKAU_000723"
     CDS             31681..32319
                     /gene="ytfB"
                     /locus_tag="ACHKAU_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO5529844.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(32303..32410)
                     /locus_tag="ACHKAU_000724"
                     /pseudo
     CDS             complement(32303..32410)
                     /locus_tag="ACHKAU_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(32454..33662)
                     /locus_tag="ACHKAU_000725"
     CDS             complement(32454..33662)
                     /locus_tag="ACHKAU_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5529845.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            33670..34095
                     /gene="tnpA"
                     /locus_tag="ACHKAU_000726"
                     /pseudo
     CDS             33670..34095
                     /gene="tnpA"
                     /locus_tag="ACHKAU_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(34159..34452)
                     /locus_tag="ACHKAU_000727"
                     /pseudo
     CDS             complement(34159..34452)
                     /locus_tag="ACHKAU_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            34724..35518
                     /gene="yjfZ"
                     /locus_tag="ACHKAU_000728"
     CDS             34724..35518
                     /gene="yjfZ"
                     /locus_tag="ACHKAU_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5529846.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(35589..36038)
                     /gene="rplI"
                     /locus_tag="ACHKAU_000729"
     CDS             complement(35589..36038)
                     /gene="rplI"
                     /locus_tag="ACHKAU_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO5529847.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(36080..36307)
                     /gene="rpsR"
                     /locus_tag="ACHKAU_000730"
     CDS             complement(36080..36307)
                     /gene="rpsR"
                     /locus_tag="ACHKAU_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO5529848.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(36312..36626)
                     /gene="priB"
                     /locus_tag="ACHKAU_000731"
     CDS             complement(36312..36626)
                     /gene="priB"
                     /locus_tag="ACHKAU_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO5529849.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(36633..37028)
                     /gene="rpsF"
                     /locus_tag="ACHKAU_000732"
     CDS             complement(36633..37028)
                     /gene="rpsF"
                     /locus_tag="ACHKAU_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO5529850.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            37355..37630
                     /gene="yjfY"
                     /locus_tag="ACHKAU_000733"
     CDS             37355..37630
                     /gene="yjfY"
                     /locus_tag="ACHKAU_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO5529851.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(37759..38445)
                     /gene="ulaF"
                     /locus_tag="ACHKAU_000734"
     CDS             complement(37759..38445)
                     /gene="ulaF"
                     /locus_tag="ACHKAU_000734"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO5529852.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(38445..39299)
                     /gene="ulaE"
                     /locus_tag="ACHKAU_000735"
     CDS             complement(38445..39299)
                     /gene="ulaE"
                     /locus_tag="ACHKAU_000735"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO5529853.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(39309..39959)
                     /gene="ulaD"
                     /locus_tag="ACHKAU_000736"
     CDS             complement(39309..39959)
                     /gene="ulaD"
                     /locus_tag="ACHKAU_000736"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5529854.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(39973..40437)
                     /gene="ulaC"
                     /locus_tag="ACHKAU_000737"
                     /pseudo
     CDS             complement(39973..40437)
                     /gene="ulaC"
                     /locus_tag="ACHKAU_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            complement(40447..40752)
                     /gene="ulaB"
                     /locus_tag="ACHKAU_000738"
     CDS             complement(40447..40752)
                     /gene="ulaB"
                     /locus_tag="ACHKAU_000738"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO5529855.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(40768..42165)
                     /gene="ulaA"
                     /locus_tag="ACHKAU_000739"
     CDS             complement(40768..42165)
                     /gene="ulaA"
                     /locus_tag="ACHKAU_000739"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5529856.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            42520..43584
                     /gene="ulaG"
                     /locus_tag="ACHKAU_000740"
     CDS             42520..43584
                     /gene="ulaG"
                     /locus_tag="ACHKAU_000740"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO5529857.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            43692..44447
                     /gene="ulaR"
                     /locus_tag="ACHKAU_000741"
     CDS             43692..44447
                     /gene="ulaR"
                     /locus_tag="ACHKAU_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO5529858.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(44444..45193)
                     /gene="yjfP"
                     /locus_tag="ACHKAU_000742"
     CDS             complement(44444..45193)
                     /gene="yjfP"
                     /locus_tag="ACHKAU_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5529859.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            45375..45704
                     /gene="bsmA"
                     /locus_tag="ACHKAU_000743"
     CDS             45375..45704
                     /gene="bsmA"
                     /locus_tag="ACHKAU_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO5529860.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            45853..46128
                     /gene="yjfN"
                     /locus_tag="ACHKAU_000744"
     CDS             45853..46128
                     /gene="yjfN"
                     /locus_tag="ACHKAU_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO5529861.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(46245..47870)
                     /gene="aidB"
                     /locus_tag="ACHKAU_000745"
     CDS             complement(46245..47870)
                     /gene="aidB"
                     /locus_tag="ACHKAU_000745"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO5529862.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(47954..49117)
                     /gene="yjfC"
                     /locus_tag="ACHKAU_000746"
     CDS             complement(47954..49117)
                     /gene="yjfC"
                     /locus_tag="ACHKAU_000746"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5529863.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(49120..49758)
                     /gene="yjfM"
                     /locus_tag="ACHKAU_000747"
     CDS             complement(49120..49758)
                     /gene="yjfM"
                     /locus_tag="ACHKAU_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO5529864.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(49768..50166)
                     /gene="yjfL"
                     /locus_tag="ACHKAU_000748"
     CDS             complement(49768..50166)
                     /gene="yjfL"
                     /locus_tag="ACHKAU_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO5529865.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(50184..50843)
                     /gene="yjfK"
                     /locus_tag="ACHKAU_000749"
     CDS             complement(50184..50843)
                     /gene="yjfK"
                     /locus_tag="ACHKAU_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO5529866.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(50894..51592)
                     /gene="yjfJ"
                     /locus_tag="ACHKAU_000750"
     CDS             complement(50894..51592)
                     /gene="yjfJ"
                     /locus_tag="ACHKAU_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO5529867.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(51611..52012)
                     /gene="yjfI"
                     /locus_tag="ACHKAU_000751"
     CDS             complement(51611..52012)
                     /gene="yjfI"
                     /locus_tag="ACHKAU_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO5529868.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(52139..52870)
                     /gene="rlmB"
                     /locus_tag="ACHKAU_000752"
     CDS             complement(52139..52870)
                     /gene="rlmB"
                     /locus_tag="ACHKAU_000752"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO5529869.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(53050..55491)
                     /gene="rnr"
                     /locus_tag="ACHKAU_000753"
     CDS             complement(53050..55491)
                     /gene="rnr"
                     /locus_tag="ACHKAU_000753"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO5529870.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(55530..55955)
                     /gene="nsrR"
                     /locus_tag="ACHKAU_000754"
     CDS             complement(55530..55955)
                     /gene="nsrR"
                     /locus_tag="ACHKAU_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO5529871.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(56160..57458)
                     /gene="purA"
                     /locus_tag="ACHKAU_000755"
     CDS             complement(56160..57458)
                     /gene="purA"
                     /locus_tag="ACHKAU_000755"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO5529872.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(57562..57759)
                     /gene="yjeT"
                     /locus_tag="ACHKAU_000756"
     CDS             complement(57562..57759)
                     /gene="yjeT"
                     /locus_tag="ACHKAU_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO5529873.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(57841..58845)
                     /gene="hflC"
                     /locus_tag="ACHKAU_000757"
     CDS             complement(57841..58845)
                     /gene="hflC"
                     /locus_tag="ACHKAU_000757"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO5529874.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(58848..60107)
                     /gene="hflK"
                     /locus_tag="ACHKAU_000758"
     CDS             complement(58848..60107)
                     /gene="hflK"
                     /locus_tag="ACHKAU_000758"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO5529875.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(60193..61473)
                     /gene="hflX"
                     /locus_tag="ACHKAU_000759"
     CDS             complement(60193..61473)
                     /gene="hflX"
                     /locus_tag="ACHKAU_000759"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO5529876.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(61550..61858)
                     /gene="hfq"
                     /locus_tag="ACHKAU_000760"
     CDS             complement(61550..61858)
                     /gene="hfq"
                     /locus_tag="ACHKAU_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO5529877.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(61944..62894)
                     /gene="miaA"
                     /locus_tag="ACHKAU_000761"
     CDS             complement(61944..62894)
                     /gene="miaA"
                     /locus_tag="ACHKAU_000761"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO5529878.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(62887..64734)
                     /gene="mutL"
                     /locus_tag="ACHKAU_000762"
     CDS             complement(62887..64734)
                     /gene="mutL"
                     /locus_tag="ACHKAU_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO5529879.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPELQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(64744..66081)
                     /gene="amiB"
                     /locus_tag="ACHKAU_000763"
     CDS             complement(64744..66081)
                     /gene="amiB"
                     /locus_tag="ACHKAU_000763"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO5529880.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(66100..66561)
                     /gene="tsaE"
                     /locus_tag="ACHKAU_000764"
     CDS             complement(66100..66561)
                     /gene="tsaE"
                     /locus_tag="ACHKAU_000764"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO5529881.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(66533..68080)
                     /gene="nnr"
                     /locus_tag="ACHKAU_000765"
     CDS             complement(66533..68080)
                     /gene="nnr"
                     /locus_tag="ACHKAU_000765"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO5529882.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            68079..69218
                     /gene="queG"
                     /locus_tag="ACHKAU_000766"
     CDS             68079..69218
                     /gene="queG"
                     /locus_tag="ACHKAU_000766"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO5529883.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(69201..69254)
                     /locus_tag="ACHKAU_000767"
     CDS             complement(69201..69254)
                     /locus_tag="ACHKAU_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529884.1"
                     /translation="MRFYFYSQAVDESGVTW"
BASE COUNT        16768 a        19009 c        17096 g        16611 t
ORIGIN      
        1 tcttcaagat cagcggcaaa cgacgcagaa ctgaatacca acgaggagac tgcaagaata
       61 gctaacaggc ttttacgcat tgctcacttc cttttttatt tacacttcat ccttcacact
      121 gcctcatcga cggctacgct cattcgcccg ggtcacgtac tgcgagtacg ctccccggcc
      181 ctcattcact ttccgtctcg atgcagtgcg aatgattttg tgtaattttt tagtttatag
      241 cgcggcaggt cgcgccagtt ttttactgcc cggcgatttt catctccggc aacagcacag
      301 aaccacactg tatattactg cgtgtttcaa tatcgttacc gacggtgaca atattgcgcc
      361 acatatcttt taaattacct gcgatggtga tttcactaac gggatactga atttcgccat
      421 tctctaccca gaagcccgct gcaccacggg aataatcacc ggtaatggca ctcacgccct
      481 ggcccatcag ctccgtcacc accagcccgg tgcccatctc tttgagcatt tgttcgaagc
      541 ttagcccttg tccggcaatc cgccagttgt gaataccgcc cgcatgtccg gtgcttttca
      601 gccccagttt ccgcgccgag tagctggtca gcagccactg agtcaggatg ccatctttaa
      661 taatatcgcg acgctcggtg cgcacacctt cgctgtcgaa tggcgtcgac gccagccctt
      721 tcagcagatg cggatgctct tcaatggtca gccagtccgg cagaatttgt ttacccagcg
      781 aatccagcag aaaggtagat ttacggtaaa ccgagccacc cgctatcgcc cccaccagat
      841 ggccaaaaag cccggttgcc acttcattgg caaaaatgac tggcgctttc atggtggaga
      901 gtttacgcgg tgacaggcgc gataaagtac ggcgagcaca gtcggccccg acccactctg
      961 gcgtttgcag atcgctcatc gcgcggccaa tggtgtaggc gtaatcacgc tccatatcgc
     1021 cattttcttc ggcaattaca cagctggaga gcgaatgacg cgttgagcag taaccctgca
     1081 acatgccgtg gctgttgcca aaaactttga caccgtagtg gctgttaaag ctgccacctt
     1141 cggtattggt aatgcgtttg tccgcctgca atgccgcctg ttctgcgcgg gccgccagtt
     1201 caatggcttc atccggggaa acttccgcag ggtggaacaa gtcgagatct ggtgcgtcaa
     1261 aggccagcag ctctttgtct gccacgccgg cacagggatc tggcgaggta taacgggcaa
     1321 tatccagcgc cgcctgtaca gtacgggcaa tggcctgcgg gcttaaatcg gtggatgatg
     1381 cgctaccttt gcggttctgg tgataaacag tgatccccag cgcgccatcg ctattgaatt
     1441 caacattctc cacttcacca taacgcgtgc ttacgctaat gccggtggtc ttgctgacgg
     1501 caacttccgc accgtccgat ttgcctgagg ccaactccag cgcagtggaa actgcttctt
     1561 ccagaatctt gcgctgcgct tcaacttgag agattacttt cattgcaagt gccataatgt
     1621 agagagtttc tctgaagtct aacagagaac cgtttttcag tgcgcatctt aactggtaac
     1681 attagcctct ttttttaagg agcctgagat gactaagcag cccgaagact ggctcgacga
     1741 cgttcccggt gatgacatcg aagacgaaga cgatgaaatt atctgggtca gtaaaagtga
     1801 aattaaacgt gatgccgagg agctaaaacg ccttggcgcg gaaattgttg atctggggaa
     1861 aaacgcgctg gataagatcc cgttagatgc ggatctgcgc gcggctattg agctcgccca
     1921 gcgtattaag atggaaggtc gccgccgcca gttgcagctc atcggtaaga tgctgcgcca
     1981 gcgcgatgta gaaccgatcc gtcaggcgct ggataagctg aaaaaccgtc acaaccagca
     2041 ggtggtgctg ttccataaac tggaaaacct gcgcgatcgt ctgatcgatc aaggtgatga
     2101 cgccatcgct gaggtattaa atctgtggca cgatgccgat cgtcagcagc tgcgtactct
     2161 gatccgcaac gcgaagaaag agaaagaagg gaataagccg ccgaaatccg cacgccagat
     2221 tttccagtat ctgcgcgaac tggcggagaa tgaagggtaa ttttcgttgt tgatgtgagt
     2281 atggtttgcc ggatgcggtg cacctgtagg cctgataaga cgcatcaagc gtcgcatcag
     2341 gcactatgct caaatgccgg atgcggcgtg aacgccttat ccggcctacg cacactatat
     2401 caggctgagg ccgaattact gcgcagcttc cgccttcttc gccagaccat ccagcagttt
     2461 ctgatggatc ccaccaaaac cgccgttgct catcaccaga atatggtcgc caggctgagc
     2521 ggttttcacc accatatctg ccagcgtatc cacatcgcca ctccagtgtg caggctgaac
     2581 gcaggcttct gccacttctg ccacctgcca cggaatatgc gccggttgca gcaggaagac
     2641 ttcatcggca cgacctaatg aaggtgccag atcgtctttg cagatcccca ttttcatggt
     2701 attcgagcgc ggctccagca cagcaataat gcgcgccgta ccaccaactt tgccacgcag
     2761 cgccgcaagc gttgccagaa ttgccgtcgg gtgatgggca aaatcgtcat ataccgtcac
     2821 gccattcgct tcaccacgca actccagacg gcgacgagcg ttaataaacg aacccagcgc
     2881 gttagcggca tctgcaggcg ctacaccaac atggcgagcc gccgcaatcg ccatcaggcc
     2941 attgtgcata ttatgttcgc ccaccagcga ccatttcact tcgcccactt tttcgccatc
     3001 cagcaagact tcccattcag aagcgtcagt ggtcagcttt ttcgcctgcc agtgaccctg
     3061 ctcacccacc agctcctgct cgctccagca gcccatcgcc atggtctgtt tcaggttgat
     3121 gtcattttcc ggccagataa tacggccctg ccccggaacg atacgcacca gatggtggaa
     3181 ctgtttctgg atcgctttca ggtcgtcaaa gatatcggcg tgatcgaact caaggttgtt
     3241 gaggatcagc gtacgcgggc agtaatggac aaatttagag cgtttgtcga agaaggcgca
     3301 gtcatactca tccgcttcga taacaaagaa gtcgctttcg cccagacgcg ccgaaacctc
     3361 aaagttcccc ggcacaccgc cgatcacaaa tcccggtttg taaccgcact gttccagaat
     3421 ccaggtcgcc attcccgcgg tggtagtttt gccgtgtgta ccggcaacgg ccagcaccca
     3481 gcggtcgcgc agcacaaaat cgtgcagcca ctgtggacct gacatataag ggatgttttt
     3541 ttccagtacc gcttccacac acggatttcc acgggtcatg gcgttgccaa taatcaccag
     3601 atccggctgc ggatcgagct ggctggcatc gtaaccctga atcagctcaa tgccttgctt
     3661 ctcaagtaag gtgctcatcg gcggatacac attggcgtcc gaacccgtta cttcatggcc
     3721 taactggcgc gccagcatcg ccagaccgcc cataaatgtg ccacaaattc ctaaaatatg
     3781 aatgcgcata cgtcactatc cttttttaat ctgccgctca ttttacgcat atgtcgggca
     3841 agtgagaaac gcatttcagg ataatccgca atttgctggc gcgattcacc tgagcgcaac
     3901 attgagatta tttgttaaga ttgttgcggt cgctttactc cataaacatt gcagggaaag
     3961 ttttatgaaa acgttaggtg aatttattgt cgaaaagcag cacgagtttt ctcatgctac
     4021 cggtgagctc actgctttgc tgtcggcaat aaaactgggc gccaagatta tccatcgcga
     4081 tatcaacaaa gcaggactgg ttgatatcct gggtgccagc ggtgctgaga acgtgcaggg
     4141 cgaggttcag cagaaactcg acttgttcgc taatgaaaaa ctgaaagccg cactgaaagc
     4201 acgcgatatc gttgcgggca ttgcctctga agaagaagat gagattgtcg tctttgaagg
     4261 ctgtgaacac gcaaaatacg tggtgctgat ggaccccctg gatggctcgt ccaacatcga
     4321 tgttaacgtc tctgtcggta ccattttctc catctaccgc cgcgttacgc ctgttggcac
     4381 gccggtaacg gaagaagatt tcctccagcc tggtaacaaa caggttgcgg caggttacgt
     4441 ggtatacggc tcctctacca tgctggttta caccaccgga tgcggtgttc acgcctttac
     4501 ttacgatcct tcgctgggcg ttttctgcct gtgccaggaa cggatgcgct tcccggagaa
     4561 aggcaaaacc tactccatca acgaaggaaa ctacattaag tttccgaacg gggtgaagaa
     4621 gtacattaaa ttctgccagg aagaagataa atccaccaac cgcccgtata cctcacgtta
     4681 tatcggttca ctggtcgcgg atttccaccg taacctgctg aaaggcggta tttatctcta
     4741 cccaagcacc gccagccacc cggacggcaa actgcgtttg ctgtatgagt gcaacccgat
     4801 ggcattcctg gcggaacaag cgggcggtaa agcgagcgat ggcaaagagc gtattctgga
     4861 tatcatcccg gaaactctgc accagcgccg ttcgttcttt gtcggcaacg accatatggt
     4921 tgaagatgtc gaacgcttta tccgtgaatt cccggacgcg taatctcctg cgcctctcca
     4981 cacggagagg cgtttttatc gctgcgcaat attgactctt gtcactggcg aactctgacg
     5041 attctcccac agcaccgtta atccacgctg taaggcaata aaaatgaaca gcaaaatgcc
     5101 gatggcgatt ttcgtccacc aggaactcag cgtaccgtca aagttgatat aagtctgaat
     5161 tagcccctga atcgccacgc caaaaagcgt ccctaatacc gttccaacgc caccgctcaa
     5221 aagcgtaccg ccaattacca ctgaggcgat agcatccagt tccacaccta cgcccgccag
     5281 cgcatatccg gcctgggtat aaatcgagaa gacaatcccc gccagcgttg ccagtccggt
     5341 ggagagcata taaatgcgaa tagtggtgct gcgggtagaa atccccatca ggtttgccga
     5401 cgttgcgttg ccgccaatgg cgtatacctg attaccaaaa cgggtacgat gcgcgaggaa
     5461 tatgccgata accaccaccg ccagcatcag cagtcccatc gcacttaagc gaccgccgcc
     5521 agggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcgggatcga
     5581 ctcttctgaa acgagatagc tgacgccgcg caaaaagaac attcctgcca gggtaatgat
     5641 aaatgccggg atcttcaggg cgtcgatcaa aagccccata aatgcgccaa aggcacagcc
     5701 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caatcacttt
     5761 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
     5821 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
     5881 attgcagatc actcttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
     5941 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     6001 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     6061 ccgcttttac gacctggttc atctccggcg gaaagccaga aagtaaaatt ccggtgttca
     6121 tcccctgaat aatcagcgcc cccacgactg agagcagcag gttaaagcgc ccgcccatca
     6181 gcgatccgcc gccaatcacc accgcaagaa tggcgtccag ctccagccat aacccggcgt
     6241 tattggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacaca
     6301 gcccgctcaa cacgtaggtg agcatgacga tgatccgcgt gtttaccccg gcattttttg
     6361 ccgcccgaat gttgatacca acggcttcga taaacatccc cagcgccgtt ttgcgggtca
     6421 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     6481 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     6541 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     6601 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     6661 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     6721 ctccggcgat agccatcacc gcccctacgg agagatcaat cccaccggtg gcgatcacca
     6781 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     6841 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     6901 cgaccagcag caccagcaat agcgccgcca gctgcggcat tcccgttggc cagcgaaagc
     6961 gccttttcgg cggcgtagtg tccgggagag attgaggcat cacactgttc tccttacgcc
     7021 gcaatggcgt tcatgatcgc cggaacggaa agctctgcca gcgggatctc cgccacctgt
     7081 ttgcgatcgc gcatgataat cacccgatcg gcatagccca ccagctcttc cagttcggag
     7141 gagatcacca acagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     7201 gcgtgggcac caacatcaat gccgcgggtt ggctcgtcga ggatcagaaa ttgcggtcgt
     7261 gtcagtagcc aacgtgaaag caacactttt tgctgattgc cgccagagag aaattcaatc
     7321 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     7381 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     7441 atattttccc gcaccgaggc ggcagcgatg atgccatcgg ttttcctgtc ttccgggcag
     7501 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     7561 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     7621 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     7681 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     7741 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     7801 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     7861 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     7921 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttcc
     7981 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     8041 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     8101 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     8161 cggcgtagaa ggccgaaacg tttgggttcg cggcctataa atagattatc agcaaccgac
     8221 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     8281 gcggtatttt tcggtgagat agcctggcct tccagccaga tggtgccgcg atcggcgtgg
     8341 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     8401 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     8461 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     8521 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     8581 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     8641 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     8701 atcaggcaga taaagggtgg atttggtcag ggtcagcttt tcaggcatgg tgccgtcttt
     8761 tttgtatttc tccagcgcgt caaaagcggg gcctgccatg ttcggcgtca gttcaacgct
     8821 ggcgttcgct tcgccatcaa tcatcgcttt atagatatcc ggtacgccgt caatggaacc
     8881 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     8941 caccatgtca tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     9001 gataaagctc tccataactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     9061 gatgattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     9121 ggcgacgctg gccccaacgg tgccctgcag ttccaccacg ttgcatggtt tgccattcac
     9181 ttcttttacc agccagtcac caatcaactt gccttcgagg atgttgtcgg cagtgacggt
     9241 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcaagcaaga agaccgggat
     9301 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     9361 aaagatcgca tccacccctt gtgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     9421 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     9481 cacattggtt tctgcagcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     9541 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     9601 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     9661 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     9721 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     9781 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     9841 cgaaaacaag caaagacatt cggcgcgagt tggctataat actcggcact tgtttgccac
     9901 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     9961 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
    10021 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
    10081 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
    10141 gtcccgactc cgtacccgct gcagccgggt tctgtgatcc gttgccgtcc ggttggcgtt
    10201 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
    10261 ctgagcaaag aatacgatca cattaaagac gttaacgatc tgccagagct gctgaaagcg
    10321 cagatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
    10381 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
    10441 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgagcttcgg ttagtaaggg
    10501 tttttttatg cccgcgataa ataaacacac cttattccac caccgcctgc aagcgtaata
    10561 acgcctcttc caccacctca tccgcagcca gaccaatacg ctttgccagc cgggcgcgta
    10621 gatccatcat ccgcacctga ttcaccagca ccacgccgtg cacatcgcct tcttcgcaat
    10681 gtaaaggaac gctaaatccg gcataacggg caaaatttcc gccctgcgta atgggggcca
    10741 ccagcgtcat tcccagttga ttaaaggctt gaacggagag cacaagcgca ggtcgaccag
    10801 caccttgctg ttcatggccg cttgctggat caaagccaac cagcacaatg tctccccgtt
    10861 caaattcact tttctttacc atatttcgtc acccgcaggg gtggatttac cccagacatc
    10921 ctgctcgcta agttccgcgg cgttcatgtc acactgtgcc agcagttcat caagcgagta
    10981 gcgcctggag atgggtgtca gaatcagttg gttgttgctc acctgcgctt ccacgctctg
    11041 ccccggctgt aagttaagtt ctttcattac gatattggga atgaccatac ctgcactgtt
    11101 cccccatctt tttatggtaa tacgcatctg cacctcctta agttatacaa agtataactc
    11161 acggtgctac aggaaacgtt ttacccctgc gcatttgtga acgccagcag agttttttat
    11221 gcgttaacag atatggaaag cggctcggag agaaaagcaa agggtgaggg aattacaaaa
    11281 cagaatgcga gtcgtctcgc aaaaaacaac gccacccgaa ggtggcgtat gcatatggtt
    11341 acttatccct gtctaaccag tcgccgcttt caattagctt taatccatcg acgggtcgtt
    11401 gataaacgta catccatgca ctcccgtacg gcgtctgaat caactggcgc gcgtattcac
    11461 cgcccctggt gcgcaaggca tcaagttcgg ccagcgtggc gttgtcaata cgataaactt
    11521 caccgtgtac tgttccgttc cccggaactg cgcctggata gtggcccagg ctatacaact
    11581 ggtagttatc gatactgaaa tcgcccagta actgggcatt ggtcatccag tgactgttgc
    11641 cttgtttgtg gcgtaaactg ccgtagacaa atattcgcat tgctaaaact cgaactgata
    11701 gagcaaatcc agtgcctggt ctacaccaga cacggcttcc agatatagct taggcatcag
    11761 gcgataacgt aacgtgagtg ttgctataga gtcaaatata cccacgccat atttcacttg
    11821 cagacctggc aatacatagc cgctgaccac tacctgggag gagtcgccta ctccctgggt
    11881 gtcgagcgct aaattgctta cgccaaacgt ctcgccgatt ttacccacaa tctggccact
    11941 ttgcgcaacc cccagaccaa tcagcatcga ggtcattgcc gcactgtcgc tctgatcgct
    12001 ctccagccct tgtccacgta gcaaataaga caatgcagct tgttgcgaca tcgccgggtc
    12061 agagaagatc tccgctttcg gttcgtccgc cagaccagtg acgcgaactc cggcgattac
    12121 gtcgtcttct gtagcatccg ggttacgaat agcttcaata ttaagatacg gttgatccgg
    12181 cggaccagag aacagtaact cacctttacg cacaatcaga tcctgaccat aggcatggaa
    12241 gcgcccttca gggatgttga tctgcccgtt caggcccagc ccttgtttgt cctgaacgac
    12301 gttaagatcg cccgtcagcc gcgctttcag gccaaaggcg tcaatgcgca cattgttgcc
    12361 aacgtggaca atcaggttac tgttaatcgg aatcgacgcc gttttcggct cttcaggttg
    12421 caggttatcg ttaagcatca ccacatcgct ggagacgcct actgcgcttt ccggcagatc
    12481 gtgcaccacg atgcgcgccc acgggacatc cacgcgacca tcgagggtaa acaggtttgg
    12541 tgtagcctcg aagacaacat ctggcgatac atccattcgt accatcggcg gcacggtgat
    12601 ccgcacttta ctgcctttcg ccgttacccg cgcccgccag ttttcaattt ggctccagtc
    12661 ggcatcaccg ttcaggtaga tttcaccctg ctgggtccgt actgtaccgg caagcgtcga
    12721 gcgcatacca ttaaagttga ccgcaagctg gctcggttgc atatcaaacg gcataaagtt
    12781 gccgtcgata tccacacccg taacctgaag ctgaccaaac aactgcgggc tttgcacatc
    12841 accacccaga cgcaagttgg cactcaccat ccccgctgct ttttccccac ggttaaagat
    12901 ggggtttatc atcgcaaggt tgaagttacg gatattgacg ttgccaccaa gattacggcg
    12961 gccttgcgga tcggtcacct gcacctgtcc atcaaactgg ccgttattgg tcaggcggat
    13021 ggtccagccc aattcggcac ggttgttacg caattccgcc gtcagattca gtgtctgaaa
    13081 cgccacgggc agcgccgcat cgttgacggt ttgcgttacc tgcacgttac gccccgaaag
    13141 ggtgatactg ccctgcggca gcccctcttt cgtggtgtcc caggcaacat ccgctttacc
    13201 cgtgaagata ccgctggcct gagtggtttc tggcataaac ggtttcagca tggcgaggtc
    13261 gaagcggttg agattcacca ccgcacgccc ttcggccccc gcatcgatag tttgcggcac
    13321 gcacagttcc gcattcgggt taagccagca gtgtggcccg atgctgattt tttgctcctt
    13381 attgcggtaa tccagcgcaa tatcgcgggt cagcgaccac gggccgaccg gcgtctggaa
    13441 gcgggtattg ctaagagttc ccttccagcg ttcttctttg cgatcaaaac ttcctgccag
    13501 attaagctgc ccggaaaccg gctcgccctg aatccgcaac tgtaactcgt gctgtttttc
    13561 gctgcctttg gcattcaggg tgacgaggtt gatatttaca tccggctgtg aaatttgctc
    13621 aacgcgcacg tcgagtttac ccgcaatctg aacggtggat ttgatatcgc cttccacgcg
    13681 aacctgcgcc acggttagct cctgccagcg caggccgcgc gcggtgatgt ccgccagtag
    13741 ttgtggcgct tccaccgtgc cgcgaacttt caccagccct ttcgctgtac cgccaagccc
    13801 cggcagcgcg ttatccagcc ccggcgcgtt gatggtggca tcaagattga gatcttttac
    13861 ccccagctcg cctttcactt cggcactgtt tggccccagt tccagatgaa gccctgggat
    13921 catccactgc atataactgt tgcctttcag cgtgccgtca acgttcactt tgttctgttt
    13981 gacgttaccg gtcagcttca gttctggcac ctccatctgc caggtgccgc cgtacaggct
    14041 accgcgggtt ttaatcaagc cattgagttt cgacggccac tctgggatct ctttggcggt
    14101 gttaatgccg ttaagcgtta gctcaccgcg ccaactaatg gcctgctgcc agtcgagcaa
    14161 cgccttgagt tcagttttcc cttccagcgc cgcgacggtg agtttgtcga gattgacctg
    14221 ctgttcatta cctttggcat caagggtaat ggtggctggc gggatctcca gtcccttcac
    14281 tgccgtacgc atagagagcg tgtaatcggt cattttgccg gtaagtttca gtttcagatc
    14341 atccgcctga tactgcttct caccggtgaa cggccagtaa agctgtttgc tgttcacttc
    14401 cacgttgagt ggcaatccgg cttccgccag tcgcgtctgg gcgcgtaaat ccatatccac
    14461 cggaccggaa agattaacgc caatctccag ctgttcgcgc agcgcgccgc ccacttttag
    14521 cttcactttt tcacctttca acggctccac gttcagtgtg ctgttgaggg tgatatccac
    14581 cggccagttg tctgacagct gcgccgtgcc gctggcgttg acgatccctt gactggaatc
    14641 gatatccagg gcgtccagtt tagtattgcc gtcaatgctg ctcactttca gcagcatggt
    14701 gcgcacggtg atgtccgtgt cgcccgtcac gcgtaactgc tcgcctttaa actcttcaat
    14761 gttcaggtta agcggcaaat gcaagtcggt catttccggc aataccgggc gagaaaagag
    14821 atctttcagc gtttcgccga gcggcttttc ctccggctgc ggattttcaa ttttcggttc
    14881 gaccacttct tcctgcgcca cttccgccac tttcggcaga gcaatcagca ggcctttcag
    14941 cgacgtcggt ttcagggtca gggttttctc ctgccagttc aggccggagg tgaagtccat
    15001 caccgacacc gtggtgtcat caatcttgat gttgacgttg tccagcgcca cccgtgtcag
    15061 ggtgatggga tacggcgtgg agagatccag cggaccgcta tcttcctctt cttcaacctg
    15121 ttcagaagga ggcatttttt tactgtcgat gttgacctga atgtctttca gcgccaggtc
    15181 attaatacaa acgctactgt tccacaggca ctcaagaccg accgccagat gcagattacc
    15241 cgcttttacc gccacgcctg gctgctcata acgaacgtca gacaaggtga gatcgcgcca
    15301 gccgccggtg accttgccaa tatccagtcc tggcacccag cgatccgccg ctttaaatac
    15361 aagatgcagg ccgctggtgg tgcccaccag aaacgccacc gatcccagca acagtaagat
    15421 aacgataacc acgccgaggc tgattttttt ccataaactc ataattctgg ccccagaccg
    15481 atgtaaaact gtaacccgtg ttcgtcttta tccgcgaccg gtacggcaaa atcgagtttg
    15541 attggcccga ccggcgattc ccagcgcacg ccgaccccgg taccggtttt aaagtcgctg
    15601 cggcgaatat cgcttaccgc ttcgccacta tcgacaaaca ccgcgcccca ccattttccg
    15661 gtcacgttgt actggtattc cagcgatccg gttatcaact tcgaggcccc tttcaggtca
    15721 ccgttggcgt atttcggggc gatagatttg tatttgtagc cgcgaatact gcgatcgccc
    15781 ccggcgaaaa aacgcagatc cggcggtact ttgtcgaaat caccggtttc aatccagccc
    15841 agcgtgccgc gtgtaacgaa acgatggcga tcgtacagtg tgcggatcca gacgttctgc
    15901 gcctggaaaa cggagaaatc gacatctgag ccccaggccg tgttggagta gtcgatagag
    15961 tagcgttgcg agtcgcccca ggttggcatc aggccaccac gagaacgcgt gcggctaatc
    16021 atcaccccag gataaaacag catcgtggtg ttggtaattt caccctgagt aaagtggtcg
    16081 agactccagc gcaggttaat ggcacgctgc cagccgctgg agagatccca gtagcgagaa
    16141 gccaccagcg tagtggagtc agattcggta tcgttcaggt cagtgcgctt aaaaccgccc
    16201 tgcaccaaat aatattgttc cagtggattc ttcagcagcg gcattttata gctgaagtcg
    16261 agggtctgtt ccggcgcgga aatactggta ctggtggtca gactgtgacc ataagagttc
    16321 atccacggct ttttccacgt agctttcacg cgcggtccca cgtccgtaga gtaaccgacc
    16381 ccggtttcga tggtgttttc agttcgcggc gaaaccacgc ccgtcaaggg taatactttc
    16441 gtttcgcgcg ctttatcaaa ttgtggagcc actacgaccg agttaaacca gccggtagca
    16501 gaaagtcgac ggttaagttc cgccagatct ttcgattcgt actcatcgcc ctctttaaac
    16561 ggcaccagat tttgcaggta ttcatcgcgg atttgtgatc cttcaaaggt cacatgccca
    16621 aagcggtaac gttcgccact gttataatca atatcccaga aggctttatg caggccgagc
    16681 gcaatgccca gctgcgcttt ggtaaattcg ctatcgaaat aacctttacg caacgcaatg
    16741 ctggttaagg actttttgaa attttcataa tcgccctggt tcagtaccgt gccaatagcc
    16801 gggcgagtat cgagcaattt caaatagtct ttatcggtcc gcgcgccgcc gcgcaatacc
    16861 acatcggtgc cgccaattaa caccggcacg cctggcgtga ctttggcgat caatacctgc
    16921 cgccctttct ttggcggtgg acggagatca aattcaatgg tcggctggta ataacccagc
    16981 gctttcagac cttcgcggat ggcatcatcg acgcgtgcgc gaaagcgacg gtctggcgtc
    17041 acttcatcac tttcaatcgt agaaagctgc gcacgaacgt tcttttccag ctgtcccgat
    17101 aacccctcga cctgtagacg gacgttcgcg gcgacggcag atccgcttaa gcagagtaag
    17161 cttacacagc ataactgtcg gatatagcgc acattttctc ctgaatatcc ttttttcctg
    17221 cccctggaac gccgttaaac ggcttaacaa aaatccagta atatggatta aaaagcagac
    17281 taaaccccaa atatttctta tgttttactt tagacctatt cacggtggtt attgtgtgca
    17341 aatacgcctc ttgttacaac cttaacccca atgaccgatt ttcgggagag cgacaccatg
    17401 agtttatttg ataaaaagca tctggtttcc cccgccgatg ccctgcctgg acgtaacacc
    17461 ccgatgcccg tagccacgct gcatgcggtc aacggtcact caatgaccaa tgtacctgac
    17521 ggaatggaga ttgccatttt tgcgatgggt tgtttctggg gtgtggagcg tctgttctgg
    17581 cagttacccg gcgtttacag caccgccgca ggctataccg gcggctatac gccaaatccg
    17641 acttatcggg aagtgtgctc cggtgatacg ggtcatgccg aagcggtacg cattgtttac
    17701 gatccttctg tcatcagcta tgagcagttg ctacaggtat tttgggagaa tcacgatccc
    17761 gcccagggaa tgcgtcaggg caatgaccac ggcacgcagt atcgttcagc gatttatccg
    17821 ctgaccccag aacaggatgc cgcagctcgc gccagtctgg aacgttttca ggcggcgatg
    17881 cttgccgccg atgacgatcg tcacatcacc acggaaatcg ctaacgccac accgttttat
    17941 tatgccgaag atgaccacca gcaatatctg cataaaaacc cgtatggtta ctgtggaatt
    18001 ggcggaattg gcgtctgtct gccaccggaa gcatagcgtt acgggtacaa atgtagattg
    18061 ttgataaagt gcgttttgtt tatgccgaat gcggcgtgaa tgccttacca ggcctacaaa
    18121 atcgtccaaa ttcaatatat tgcagggact gcgtaggcct gatcggcata gcgcatcagg
    18181 cagtattgca tttatcagca gtctgaattt taaccctctg gcgactttac agtaccttac
    18241 gctatactag ccactgaaaa tgccggttca ctttcttcga atcggctttc aatgtgtatt
    18301 tcacacaaat taatcaactt ccccttccga ggatccggcc tgaaaggtcg gataagatat
    18361 gttaaacagt attttagtca tactctgctt gatcgctgta agtgcgttct tctcgatgtc
    18421 tgagatctca cttgccgcct cacgcaaaat caaacttaaa ctgctggctg atgaaggcaa
    18481 tataaatgcc caacgcgttc tgaatatgca ggaaaatccc ggcatgttct ttacggtggt
    18541 ccaaatcggt ctgaacgcag ttgcgattct cggcggtatc gtcggtgatg cggcattttc
    18601 tccagctttt cacagcctgt tctcccgcta tatgtcggca gaactctctg agcaactgag
    18661 ctttattctc tctttctcgt tagtgactgg catgtttatc ctgtttgcgg atttaacccc
    18721 gaaacgcatc ggtatgattg cgccagaagc ggtggctttg cgtatcatca acccgatgcg
    18781 cttctgcctg tacgtttgca ccccgctggt gtggttcttc aacggcctgg cgaacatgat
    18841 cttccgtatt ttcaaactgc caatggtacg taaagatgac atcacttctg atgacatcta
    18901 cgcggtagtg gaagccggtg cgctggcggg cgtgttacgt aaacaggaac acgagctgat
    18961 tgaaaacgtc tttgagctgg aatcccgtac cgttccgtct tcaatgacac cgcgtgaaaa
    19021 cgtgatttgg tttgatctcc acgaagatga gcaaagcctg aagaataagg tggcggaaca
    19081 tccgcactct aagttcctcg tctgtaatga agatattgac cacatcatcg gttatgtcga
    19141 ttctaaagac ctgctgaacc gcgtgctggc taaccaaagc ctggcactga acagcggcgt
    19201 acaaattcgc aacacgctga ttgtgccgga tacgttaacc ctttcagagg cgttggaaag
    19261 ttttaaaacc gcaggtgaag acttcgcggt gatcatgaac gagtacgcgc tggtggtggg
    19321 gatcatcacc ctcaacgacg tgatgaccac gctgatgggc gatctggtcg gtcaggggct
    19381 ggaagagcag attgtcgccc gtgatgagaa ctcatggctg attgacggcg gcaccccaat
    19441 tgacgacgtc atgcgcgtgc tggatattga cgagttcccg cagtcgggca actacgaaac
    19501 catcggaggc tttatgatgt ttatgctgcg taagatcccg aaacgcaccg attcggtgaa
    19561 attcgccggc tacaaatttg aagtggtgga tatcgataac taccgcatcg accagctgct
    19621 ggtgacccgg atcgacagca aggccaccgc cctttcgcca aaactgcctg acgctaaaga
    19681 taaagaagaa agcgtcgcgt aacccccaga aacatcaacg gctcctgaat caggagccgt
    19741 cttattactg catagcactt tggttaagcc atctctgttt gcagacgcat aacctgacgg
    19801 ttaacttcgg acatcactga caaatgcagc ttatccttca cttttgggat aagaatctta
    19861 cccttatcaa attcaaaagc gccaacgtcc ttgatgtata accgtccacg gaacaggatc
    19921 tttacgtact tcgccacctg aagtgggttg taccgttgga aaattttcat tgttatctcc
    19981 tgctgagtat tacgcctgtg cggtgccaca atcggcccaa ctattatgag gcgcaaattt
    20041 taatgcctag tgactataga ctatccgggc aatgtttcca ccgcgtataa ctttttttac
    20101 ctaaaggtta caattattca gaattatctt tttaccgaag cgcggtcttc agtataagca
    20161 ttcatttttc atatgatttg tgcgcttgac cgcaaactgg catcacactt gcgggaaatt
    20221 cgataaatag cacatatgat taaaactcag acccaagtgg tcggatcacc tgcatatcat
    20281 aagaaggaaa caccatgacc ctacgcaaga ttctggcact cacctgcctg ctgttgccga
    20341 tgatggcttc cgcacatcag ttcgaaaccg gtcagcgagt gccgccgatt ggcatcaccg
    20401 atcgtggcga gttggtgctt gataaagatc agtttagcta caaaacctgg aacagcgcgc
    20461 agttagtggg aaaagtgcga gtactgcaac atattgctgg tcgcacctct gcaaaagaga
    20521 aaaacgcgac gctgattgaa gcgattaaat cagcgaagtt accgcacgat cgttaccaga
    20581 ccaccaccat tgttaacacc gacgacgcaa ttccgggttc cggcatgttt gtgcgcagca
    20641 gtctggagag taataaaaag ctttatccct ggtcgcagtt tattgttgat agcaatggcg
    20701 tcgcacgcgg tgcctggcag ctggatgaaa aaagttccgc tgtagtggtg ctggataaag
    20761 acggtcgcgt gcaatgggcc aaagacgggg cgcttactca ggaagaggtg cagcaagtga
    20821 tggacctgct gcataaatta attaataaat agacactctg aacccaggat tcaggaacga
    20881 ctcgcgcgga gtgtaatcca gcggtttacc ctgccagtcg tgaacgtgcg ctccggcagc
    20941 tgcggccaca gcatgtccag cggcggtgtc ccaaatattc gttggtccga agcgcgggta
    21001 cagctgcgcc tgtccttccg ccaccaggca gaatttcagc gaagagccga tggacgtggt
    21061 ctgatgttcg ccaagctgtt gcagatactc tttcagctcc gcatctgcat gggaacggct
    21121 gataaccacc agcggcgggc gcgcatcgcg gacctgaatc tgcttgcgca caccgcactc
    21181 ttctttccag gccttgcctt ctgccgcgct gtacattacg ttcattaccg gcgcatacac
    21241 cacacctaaa atcggtttgc catggtcaat gagcgcaatg ttaacggtga attcgccatt
    21301 gcgtttaata aactctttag taccatccag cgggtctacc agccagtaac gctgccagtg
    21361 ctgacggact tcccaaccgg gaggatcttc ttcagaaagg accggaatat ccggtgtcag
    21421 cgtacgtaaa ccgtccatga taacggtgtg agcggcaata tccgctgccg ttaccggaga
    21481 attgtcagct ttgctgacga cgtccatcgg tttcgtcccg tcgtagacct gcataatggc
    21541 atcgcctgca ttccgtgcaa gctggcatac ttgatctaac atttctccac ctcgtctctg
    21601 tgagcggtgt taacttattg ttttacttat accctatcgt taatgaatgc gccaactgtg
    21661 atagtgtcat cattttcaaa gcgtaaaatt gtggcattct tcactgttct ataagtaaga
    21721 cgtttattct cccttttctt tcgtattccc gatgataaaa ggatgtccct gatgattaag
    21781 tttagcgcaa cgctcctggc cacgctgatt gccgccagtg tgaatgcagc gacggtcgat
    21841 ctgcgtatca tggaaaccac tgatctgcat agcaacatga tggatttcga ttattacaaa
    21901 gacaccgcca cggaaaaatt cggactggta cgtacggcaa gcctgattaa cgatgcccgc
    21961 aatgaagtga aaaacagcgt actggtcgat aacggcgatt tgattcaggg gagtccgctg
    22021 gccgattaca tgtcggcgaa aggattaaaa gcaggtgatg ttcatccggt ttataaggcg
    22081 ctgaatacgc tggattatac ggtcggtaca ctcggcaatc atgaatttaa ctacggtctg
    22141 gattacctga aaaatgcgct ggcgggagcg aaattccctt atgtaaatgc caacgtcatt
    22201 gacgccagaa ccaaacagcc aatgtttaca ccgtatttaa ttaaagatac cgaagtggtc
    22261 gataaagacg gaaaaaaaca gacgctgaag attggctata ttggcgtcgt gccgccgcaa
    22321 atcatgggct gggataaagc taatttatcc ggaaaagtga cggtgaatga tattaccgaa
    22381 accgtgcgca aatacgtgcc tgaaatgcgc gagaaaggtg ccgatgtcgt tgtcgttctg
    22441 gcgcattccg ggctgtctgc cgatccgtat aaagtaatgg cggaaaactc agtttattac
    22501 ctcagtgaaa ttccgggcgt taacgccatt atgtttggtc atgctcacgc cgttttcccg
    22561 ggtaaagatt ttgctgatat cgaaggggct gatatcacca aaggcacgct gaatggtgtt
    22621 ccggcggtaa tgccgggcat gtggggcgat catcttgggg tggtcgactt acaactcagt
    22681 aatgacagcg gtaaatggca ggtgacgcag gcgaaagcgg aagctcggcc gatttacgac
    22741 atcgccaata aaaaatccct cgcggcggaa gacagcaagc tggtagaaac actcaaagcc
    22801 gatcacgatg ccacacgcca gttcgtcagc aagccaatcg gtaaatctgc cgacaatatg
    22861 tatagctatc tggcgctggt gcaggacgat ccgaccgtgc aagtagtgaa caacgcgcaa
    22921 aaagcgtatg tcgaacatta cattcagggc gatccggatc tggcaaaact gccggtgctt
    22981 tcagctgccg caccgtttaa agttggtggt cgcaaaaatg acccggcaag ctatgtggag
    23041 gtggaaaaag gccagttgac cttccgtaat gccgccgatc tttatctcta ccccaatacg
    23101 ctgattgtgg tgaaagccag cggtaaagag gtgaaagagt ggctggagtg ctccgccgga
    23161 cagtttaacc agattgatcc taacagcacg aaaccgcaat cactcatcaa ctgggatggt
    23221 ttccgcacct ataactttga tgtgattgat ggtgtgaatt atcagattga tgttacccag
    23281 cctgcccgtt atgacggcga gtgccagatg attaatgcca atgcggaaag gattaagaac
    23341 ctgaccttta atggcaagcc gattgatccg aacgccatgt tcctcgttgc caccaataac
    23401 tatcgcgctt acggcggcaa atttgccggt acgggcgaca gccatatcgc ttttgcttca
    23461 ccggatgaga accgctcggt gctggcagcg tggattgctg atgagtcgaa acgtgcgggg
    23521 gaaattcacc cggcggcaga taacaactgg cgtttagcac cgatagctgg cgataagaaa
    23581 ctggatatcc gtttcgaaac ttctccgtca gataaagccg cagcgtttat taaagagaaa
    23641 gggcaatatc cgatgaataa agtcgcgacc gatgatatcg ggtttgcgat ttatcaggtg
    23701 gatttgagta agtaaaacac ttctttttcg gcctataaat catcaaccgc atccggcatt
    23761 tattggcgga tgcgatgctg gcgcatctta tccgccctac aagccatgca ccgtagacca
    23821 gataagctca gcgcatccgg cagttatgcc gcacgttcat cccgcaccgc caacacctcg
    23881 ggcaaattca actcaatcca gtccgccagt gcagcaacct tttcgctcac ctgctcgccc
    23941 agcggcgtga ggctatattc cacatgcggc ggcaccaccg gatacgcgat acggttaaga
    24001 aaaccatcct gttctaacgc ctgtaacgac tgcgcaagca tcttttcgct caccccgccg
    24061 attttgcgcc gcaggtcgct aaagcgatga gtaccttcgc gtagcgccac cagaatcaac
    24121 accccccaac ggctggtgac gtgtttcaac acctcgcgcg acgggcactg ttccgcaaag
    24181 agattaccct ctttcagttg ttgcgacagg ctaacctgac tcatttcata cttacctttt
    24241 tgtgcgtact tactaaaagt aagtttaggt gttagcgtat ttaaacacaa gacaaaacga
    24301 tggagacttc ccatgatcgc tattactggt gccactggcc aacttggtca ctatgttatt
    24361 gaatccttga agaaaacggt tcctgtcagc caaatagtgg ctatcgttcg taatccggca
    24421 aaagcccagg ctctggcagc acaaggcatt accgtgcgtc aggctgacta cggcgatgaa
    24481 gccgcactga catctgcact tcagggagtg gaaaaactac tgctgatctc ttccagcgaa
    24541 gtgggtcaac gtgccccgca gcatcgtaat gttattaatg ccgcaaaggc ggctggtgtg
    24601 aaatttatcg cttataccag cctgctacat gcagatacct ccccgctcgg cctcgccgat
    24661 gagcacatcg agacggagaa aatgttggct gattctggca tcgtttacac cctgctgcgc
    24721 aacggctggt acagcgaaaa ctacctcgcc agcgccccgg cagcactgga acacggcgta
    24781 tttatcggtg cggcgggcga tggcaaaatc gcctcagcaa cgcgggcaga ttatgcggca
    24841 gctgcggcac gcgtgattag cgaagccggt cacgaaggca aggtttacga actggcgggc
    24901 gatagtgcct ggacattgac acagttagcg gcagagctga ccaaacagag cggcaaacag
    24961 gttacctatc aaaatctgag cgaagccgat ttcgccgcgg cactgaaaag cgtcggactg
    25021 cccgacggac tggcggatat gctggcggat tctgacgttg gcgcatcgaa aggtggtctg
    25081 tttgataaca gcaaaacgct tagcaaattg attggccgcc caacgacaac gttagccgaa
    25141 agcgtaagcc atctttttaa tgttaataac tagttaatta aagtggcatc ctcccgcatc
    25201 ctctctaata atgacgggat gccgggagca atcatgtctg cttcctgaac tttcttctga
    25261 cagaccaatg gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga
    25321 tgtgggggct tatttttgtc gggccgttga tcgtaccgga atacccggcg atgttgcagt
    25381 cgatggggcg ttatctggcg ttagggttaa ttgcgctgcc cattgcctgg ctgggacgcg
    25441 tgcgtctgcg tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg
    25501 gcaacctcat ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt
    25561 ccacgatgat tatcggcacc ctgccggtgg tcattcctgt ctttgccaat ctgctttata
    25621 gccagcgcga cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca
    25681 tcggcctggc gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg
    25741 cacgttatac ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg
    25801 ccctgcgcaa cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga
    25861 cggcgcaggc gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact
    25921 ggctgaatat acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta
    25981 ttagtctgat ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg
    26041 tcgccagcca gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg
    26101 caggtttgct gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg
    26161 gtatcgcgct gttagtgatt ggcgtggtca ttgcggtcag agcaaaacca gaaaaacctt
    26221 taactgaatc tgtctcagaa agttgacacg ctggcagtga gttaaataag cctctgctac
    26281 gtaagggtta tagcttttgc cttaaagatg catttaaaat acatcttatc ttattaagaa
    26341 tgaggtatca gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc
    26401 tcgcgcttcg gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac
    26461 gctggcgcgc gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc
    26521 aaagcttgct gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat
    26581 tgaccatatc atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct
    26641 gcaagcgact aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac
    26701 aaaatacctg actatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat
    26761 cctcttcccg atgatcaaac aaggcatggg cagccaggca atgggaccaa tcagcgtgat
    26821 ggaaagcgag cacgatgaag cgggcgaact gctggaagtg attaaacaca ccaccaataa
    26881 cgtcacaccg ccgccggaag cctgcaccac ctggaaagcg atgtataacg gtattaatga
    26941 actgattgat gacctgatgg atcacatcag cctggaaaac aatgtactgt tcccacgcgc
    27001 gctggcgggt gagtaatttg ttcgatcaac gcacctggtt tacacaatca ggtgcgtttt
    27061 caaatgcttt gagttattca ccactccggg acaatctttt gtatctactg aactgggaaa
    27121 attcattatt cagccaataa taagttcctt caacggttac taacttattt ttttcattaa
    27181 tataattact tattcatcgc tcctgtataa gccagaataa aatcacccat tccctggttt
    27241 ttacgtgtac atccagctac cgctacgctt ccacccacac cgacccgcca tcatggcggt
    27301 tcatcagcga ccacagcaca tggaatttct cgcctctttt atactccccc tccattttcc
    27361 tgtccgtcca ttctggcatc tgctctccct gctccagtgt caccgtcgct tgtctacctt
    27421 ctacaaaaat cgaccactcc ccgctatgag gacaagcttc accgctaaag gccgctatcc
    27481 gctgccggtc tgtaacatac tgcgcttcgc gctgtggcca gttgtacaac tcctccggca
    27541 tgccctgtgg gaaaaatccc tccggcggca cattgataaa tcggtttgcc acccgccgta
    27601 tacgtttcca attgcatttt gcccacgccc cgctttccca gtaatattct tgtatcccag
    27661 cctcgttgac atacttcgtt cgtacccgcc cctgccaagc ctcgggaata tgttcactcc
    27721 ggtacaacag ttgggcgctg gcatctggga tatccggcag atacaggcca gccaccgtta
    27781 ccgagtcatc ctcacgcagg tatacgctac tatccagctc atacaacggt aattgcatcg
    27841 gcagggccag cgggccgtgc agactttcct cataataata agtcagttcg ccgtcatacc
    27901 agtatccgcc cgaggtacgt ctgattaaat tattataatg tgcacctcgc catttcagtc
    27961 tgtcatactg tttttgcgcg gcgagtgaca tccagtccga cggataatca attagtcctt
    28021 tcatatcact gctaatattg cccccggatt gcagtacccg caggtccccc tgctgtagct
    28081 gaataaagtc atactgtacc gactttaaag tactgcggat attgggcaac actcgcccgc
    28141 cccagacgac gtcaatctgc tcgcacagcg gacgacggcg cacatccagc ggcatggtct
    28201 tgctccactc tgccacctgt cgctcgcaca gctcgacaaa ctcctgccag ccctcaaaac
    28261 gacgccgata atattccggc gacattattc tttccagcaa ccagcactcc tgtggatgta
    28321 atatagccac tttatctcct gatttattaa ttaattgtct attgatactt ttatttcggt
    28381 acataaatat ttgtttgcaa ctgagacaca gctatataca tatccacttc atctatcatc
    28441 ggtacgctga ccgtcaaaat agacgctgcg tttatcggca gcgaaagcac cgaatgcctg
    28501 aaaactggcg gcatcgacat taggttcacc gggcggattt ttcaggactt tgccagacca
    28561 aagaacagag cgtccatcgg tgagccaggt atgctggcaa ttattccgat catcgcaatc
    28621 gaacttatcg ggattaaaat cggacagtaa gcgggtttct tggttattgg ctaaattggc
    28681 aatacgccag aggcgggaaa aattaacgcc cggcagttcg gagaggatct catccttttc
    28741 tcccttctgt tcggcaacat aaaccgtgcc tttctcctgc tggcggtaat aaaaatctgc
    28801 gccccaggaa ggtgaaatgg agagaggtaa tgcggcgatt aataaccaac tgtgcttagc
    28861 catagtgaac tctgcaatga gtggcaaaga tccacggtaa gcccgcgcct gagagctgtc
    28921 gatgacggga agtattttta ggaatgtata aagacaaagg agattgctga caaagtgtgc
    28981 tttgttcatg ccggatgcgg cgtaaacgcc ttacccggcc tacaaaagca cgcaaactca
    29041 atatattgca gaggttctgt aggcctgata agcgtagcgc atcaggcaat tttgcagcct
    29101 gaaagacacc cgcaggtgcc ttagcggcat tacttcgcca tgcgtttttt accaataaac
    29161 agccagccca accccagcgc gataaaccac agcggggtaa ccagcagcgc ctggcgagtg
    29221 tcatcttcca gtgtcagcag cacgatcacg aacacaaaga acgccataca tacccagcac
    29281 atcagcttgc cgagcggcat cttgtagatc gacttctcat gcagatgagg acgctgtttg
    29341 cgatacacca ggtacgagca aaggataatc gtccagacga acataaacag aatcgcggaa
    29401 accgtcgtga tcatcgtgaa cgcgccaatc acgctgggat tcacatacag catcaccacg
    29461 ccgccgagca gacagatgca ggagaaggtc agccctttcg cgggtactgc gcgcttagag
    29521 agtttagcga acgctttcgg tgcaacacct tcctgggcca ggccaaacag catacggctg
    29581 gtagagaaga cgccgctgtt agcggaagac gccgcagagg tcagcaccac aaagttgatc
    29641 acgctggcag ccgcaggcaa acccaccaac acgaacagtt caacaaacgg gcttttctcc
    29701 gggactaccg aactccacgg cgtcacggac ataattacaa tcagcgcgaa gacgtagaac
    29761 ataatgatac ggatcggaat agagttaatt gcacgcggca gtgatttctc cggatctttg
    29821 gtttccgcag cggttgtacc taccagctca atccctacga aagcgaaaac cgctatctgg
    29881 aatccggcaa agaagccact taaacctttc gggaaccagc cgccatcatt ccacaaatgc
    29941 gcgaatgacg cttccacacc agtcggtgac tgaaagtgca tcgccaccat aaccaggccg
    30001 acgacaatca gcgacacaat ggcgacgatt ttgatcatcg caaaccagaa ctccatctca
    30061 ccgaacattt tcacggtggc gagattgagc gtcagcagca gcactatcac cgccagcgag
    30121 gcgacccagt cggagagatc ggggaaccag aactgagcat aagccgtgat cgccaccacg
    30181 tctgccatac cggttacaac ccagcagaac cagtaagtcc agccggtgaa atatcctgcc
    30241 cacggcccga gtaaatcgga agcgaagtca ctaaaagatt tgtattccag attcgaaagc
    30301 agcaattccc ccattgcccg catcacgaaa aagagcataa aaccgatgat catataaacg
    30361 aaaatgatcg acggcccggc aaggctaatc gttttgccag accccataaa caaccccgta
    30421 ccaatggcac cgccaatggc aataagctga atatgtcggt ttgtgagatt gcgccgtagc
    30481 gactgttcag ccggagcctg atcatcggca acgactttta cctgatctac catgtttttt
    30541 tcttcctgta cctgtctgtg ttgttcaggc tctacggcct ttagtcagcc tatgatacga
    30601 cgaatctgta tcgggactca tcgatattag gtaacaatac gcgggatgaa tactaagatt
    30661 tagatctaat gttaatttta tgtttaaagt gagtgtcata tcacggtgat aacgcgaaac
    30721 agctcacaaa aatacacgct tacggggtct ttgcaacaca aaatagcgcc gtttggcaaa
    30781 ctgaagggtt tattgctgaa tgcctgctcc cctctggttc gaggggaaca gaatgcgctg
    30841 cttagaggat ttccagcagt tcgacttcaa acaccagggt gctgaacgga gggatggatg
    30901 cgcctgcgcc gcgctcgcca tatgccagtt cctgcgggat agtcagttcc catttagaac
    30961 ctaccggcat cagagtcagt gcttcaatcc agccagggat cacgccatta accggaaatt
    31021 cagcgggttc accacgagca acggagctgt caaacacggt gccgtcgatc agtttaccgg
    31081 tgtaatgaac acgaacgcgg tcggtacgtg ccggaattgc gccttcaccc tggttgatca
    31141 cgcggaattg caggccagat tcggtgctat tcacaccttc ttttttggcg ttttcttcca
    31201 ggtatttcac accttcagca gccatcgcct ggaaacgctg acgacgaacg gcatcggcgc
    31261 gctcgtggat ttcacgcagc gcgcgatgca ccacatcaac cggaacagcc ggatgtttgc
    31321 cttccagcgc atcggcaata cctgcaacca gtgcttctgg cagcagccct tccaggccag
    31381 attcactcag ttgttgccct acctgcaaac caatgccgta gcttgcttgc gcttcgatgg
    31441 tgtcaaaagt tggggtggtc atcgtatttc ctttcttagg gtataaaagt agcgggcagc
    31501 atatcagcca cacttctttg ggtaaaactt tgtcgcagga atgatgacaa atcccgggga
    31561 aaaagaaaca atagcaatat cccgtatgaa tataataaag gcgggtttac cacggacatc
    31621 atagcgttag cttattcata ggctatgatt gaggaacaag acgcggagca ggaggaaacc
    31681 atgcccgggc gctttgaact aaaaccaacc ctggagaaag tctggcacgc gccggacaat
    31741 tttcgcttca tggacccgct gccgccgatg catcgtcggg ggatcattat tgccgccatt
    31801 gtattggtgg tcggatttct gcttccatct gatgatacgc ccaacgcgcc ggtggtgacg
    31861 cgtgaagcgc agttagatat tcagtcgcaa tcacaaccgc cgacagaaga acagctgcgg
    31921 gcgcaattag tcaccccaca aaatgatcct gatcaggtgg ctccggtcgc tccagaacct
    31981 atacaggaag gccagccaga agagcagccg caaactaccc aaacgcaacc attccagcca
    32041 gatagcggaa ttgataatca atggcgctct taccgtgtcg aacccggtaa aacgatggcg
    32101 cagctgttcc gcgaccacgg actgcccgct accgatgtct atgcaatggc gcaggtagaa
    32161 ggcgcgggta agccgctgag taatttgcaa aacgggcaaa tggtgaaaat ccgccagaac
    32221 gccagcggcg tggtgacggg cttaaccatt gatacgggga ataatcaaca ggtcctgttt
    32281 acccgccagc cggatggcag ttttattcgt gcgcggtaga tgaagggtca accaaaagcc
    32341 agctaaggat tgtctctttg atgaccttac gtctggcttc cagtgcctgg ggggaaacca
    32401 tactgatatg ttgacatcct ccacgccctg aaggacgtgg atccctgctt cgttcaggct
    32461 gtcgcctgaa tcatttcggt gggttcctgc ttcaacgagc ggcctgactg caccatctct
    32521 ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac gagcgccgtt tacatcggcg
    32581 ttcgctgtat atccacatac ctggcatctg aatttacttt gtgacaggcg attttctttc
    32641 gctgtatgac cacagcacgc gcaacgctgg cttgtgtacg ctggcggaac agcaagcacc
    32701 tgaccgccac gccagagctg cttgtactca agctggcggc gcatttcata ccagccctga
    32761 tccagtatcg aacggtttaa acctgatttt gcccggacat tgcgccccgg ctggctgacc
    32821 gtacccgctg ctgactttga catgttgctg accttcaaat cctcaatgac tatcattgcg
    32881 tggtttttgc tgacggtcgt tgtgacttta tgaaggtagt ccctgcggat atttgcgata
    32941 cgggaatgca gtcgctgtat tttgcgtttc tgcttctgcc agttgttgct gaatttgacc
    33001 ctgcggctta actgtcgctg aagtctcgcc agcgtcttct ggtttttctg aaaactgttt
    33061 acaggctcaa agactgtgcc atctgacagc gtggcgagtt tagccacgcc agcatccagc
    33121 ccgaccattg atgctgaagg gtgaaccgga gtggatactt cactttctgt ctgaatactg
    33181 atgtaccact taccgcagga ctggctgaca gtgacatttt tcacaactcc cgtgacctga
    33241 cggctattcc ggtagcgcat ccagccaagt ttcggcagaa aaatacggct gttttcctga
    33301 tcgagcttaa cgccctgcgg gtagcggaat gcatcattct gtccccgctt tttgaatcgg
    33361 ggaaaagcag cccgattctg gaagaagttt ttgtaagccc gctcaaggtc tttcagtgac
    33421 tgttgcaatg gctgtgaggg cgaatcttta agccattgcg tttcagtggc gtttttccac
    33481 tcaaccagcc aggaagccat tttcccgtaa gggatgtatt tattaccggc ctcatgattc
    33541 tcattctgac gtgccagagc acgattgaaa acgaaacgac atgcgcctgc gaagcgcctc
    33601 atttcacgct cctgttgacc accgggtctt aactggaatt taaatgcctg tagtcgttcc
    33661 acaggtcaaa ttatattttg gtctgtgaaa aaagaaaccg atatccggcg tggaaggcat
    33721 tgtgttttcc tgatgcatgt tcacctggtc tttgtcacca aataccgacg ccagattttt
    33781 gaccatgacg cgacagaaaa actacgcact tacttttcaa atgtatgtgc tgattttgaa
    33841 gctgaactgg ttgaaatgga tggcgaacca gatcacgtcc atttgttaat tagctatcct
    33901 cccaaactgg cgatatccag tctggtaaac agcctcaaag gcgtatcggg taggttactg
    33961 cgacgagatc gaccagatat tgcagtcagg tattactaca aaggcgtttt gtggagtcct
    34021 ggctattttg ccaatagctg cggaggtgcg ccaatatccg tcatccgcca atacattgaa
    34081 caacagcaaa cacctggtca ggtggaaaac cgcgccttat atccccgccc tgaaggacgg
    34141 ggttttacgg cgcaccagat aaaccaggcc aagcgtatgc tggttgatca aataatatcc
    34201 cccaagcgca gcaatattaa tattcacctg caatgggtcg atacctggct taaaaatatt
    34261 gtatttcttc ccttcatcca ataaggattc cattaactgc aagtgcgcat ggttaatttc
    34321 taagagacgc tgtgattttg cataatgcac acctttactt tgattttcac tatgcacaat
    34381 ttttaaaaac caagggttag caatataata accccatgta aaatcaataa ttttctcaat
    34441 ggctttttgt ggctggttaa ccagatgatt aacaaacgca ccgataccaa cagcgtagaa
    34501 taattacata ccgcacaaca agaatgtatg gtggaaggcg tggaagtcat tttctccccg
    34561 ggggcatcgt tatatccata atgagcgatt tttgatatta atatctttca ccatccgcta
    34621 ttttcactac ttcagaaaac aaacttattt ttattaatat cgattctttt tctgtgaaca
    34681 tcagctgcga tatttataaa aacattaaac aggtaacatt acaatgacat taccaacgac
    34741 tatttattca tttcctgcat attcacccca tttttcgtcc accgacaaac cagtaaaatt
    34801 aaaatttcat caatatgcac gtgctacatt actgccaaat cgtggaagag atcataattg
    34861 cgatggcaga cgtactgttg agatccacaa actagatctc tctgactggc aagcattcaa
    34921 taagttagca acgaagtgta atgcttacga tggagtaaca atgaatggtg ataattcttt
    34981 tggatggaac cataaagcta cattagataa tattcacgcc cagaaataca ataaagctca
    35041 cgcgggtgcg aggttaaccg cagaactgaa atatttattg caagatgtcg aatccttcga
    35101 acccaacagt aaatacacaa tacacgaagt ggttttggga cctggttatg gtactccaga
    35161 ctataccggc caaacgatag gatacgttgt aacccttcct gcccaaatgc ctaattgctg
    35221 gtcatctgaa ctaccaacaa tcgatcttta tatcgaccaa cttcgcactg ttacaggcgt
    35281 ttctaatgcc ctcggattca tcattgctgc attgctaaac gcgtattctg atttacccca
    35341 tgatttgaaa attggtctac gttccttatc aaattcagca gcaatctact cgggtttagg
    35401 tttcgagcga gtgccgcagg accgagacat aaggagtgat cgtatgcatc taacgccagc
    35461 aaaccatcct gacttatgga cacaagagaa tggtgaatgg atctacttgc gaaactaata
    35521 cctgacgaaa aatagaaagc aaaacgccga ccaatggtcg gcgtttttac gtctcgttga
    35581 ataacgaatt attcagctac tacgtttacg atcactttcg cgaatacttc gctgtgaacc
    35641 tggaagctca cttcgtgttc gccagtggta cgcagaacgc cgttcggcag acgaacttcg
    35701 ctcttagcca cttcaacgcc agctgcagtt acagcgtcag cgatgtcgcg agtaccgatg
    35761 gaaccgaaca gtttaccttc gtcgccagct ttagacgcga tggtaacagt ttccagtgca
    35821 ttgattttct cagcgcgagc attagcagct gccagaactt cagccagttt agcttccagt
    35881 tcagcgcgac gtgcttcgaa gaattcaatg tttttcttgg tagctggaac agctttaccc
    35941 tgcggtacca ggaagttacg agcatagccc gctttaacgt ttacctgatc acccaggcta
    36001 cccaggtttg ctactttatc aagcagaata acttgcatta ccttatcctc tcaaagtcgt
    36061 attaatggac cgtgaccgat tactgatggc gatcagtgta cggcagcagg gacaggtagc
    36121 gagcgcgttt gatagcgcga gccagctgac gctggtattt tgcacgggta ccggtgatac
    36181 ggcttgggac aatcttaccg ctttcggtga tgtagttttt cagcgtagcg atatctttat
    36241 agtcgatctc ttgaacgcct tccgcggtga aacggcagaa cttgcgacga cggaaataac
    36301 gtgccatatg gctagtctcc agaatctatc aattcaatct gctcggcatg caaaaccatt
    36361 ttgctcagtc cgttctttgc cttgtggcat gaaatgaacc cctgaacggt tatgcgactg
    36421 ccgaccgtta tactgtgagt aatggcctgg ttttcgtgtc cgctaacaat aacgggcatt
    36481 tgacaccatg cctgccggtg aaagccggct tcctcctgca cagaacgatg ctcaagcacg
    36541 aactggcagt gaggaattcc tgatggactg acctttcgaa ggggagtcct gcacacggtg
    36601 ccggacaaca ccagacggtt ggtcatcaga aattactctt cagaatcccc agcttcagca
    36661 tcatcagcgg tttcgtttgc gaaatcatcg cgacgctcac ggcgctcgtc tttcgcttta
    36721 accatcggag atgcttcggt aacagcgtgc ttggtacgca taaccatgct gcggataacg
    36781 gcatcgttga agcggaaggt agtttccagc tcatcgatca cttcctgcgg agcttcaacg
    36841 ttcatcagaa cgtagtgtgc tttgtgcagt ttgttgatcg ggtaagccag ctgacggcgg
    36901 ccccagtctt ccagacggtg gatcttgcct tctgcaccag tgatggcagc agtgtagcgc
    36961 tcgatcatgc ccggaacctg ttcgctctgg tcaggatgga ccataaaaac gatttcgtaa
    37021 tgacgcatcg aattgctcct tacggattat tcagcctcct gtctgggtca gccgaatccc
    37081 ggggaggcaa ggaacgtgtt aaaggtcggc tgaaaaatga cgcgttatag tacttgtatg
    37141 ccccgctaaa ctcaaggcca gccgacaaat aaatctcatc tttcgctcaa aagaactaac
    37201 tccctgattt gaagttcatc acattccgtc acagtgtttt ttcgaacaac tgcatcagaa
    37261 atggtcacca tcaacttctt tatcattgat taaaattaaa tcagaagatc atcacagagg
    37321 agagcacaca aagtcagccg ttgaggaggt atctatgttc agtcgtgttt tagcccttct
    37381 ggctgtgctt ttgctaagtg caaatacatg ggcagccatt gaaatcaata accaccaggc
    37441 cagaaatatg gacgatgtgc aaagcttagg cgtgatttat atcaatcata atttcgccac
    37501 tgaaagtgaa gcacgtcagg cattaaatga agaaacagat gcgcagggcg caacgtacta
    37561 ccacgtaatt ctgatgcggg aaccggggag taacggcaat atgcacgcca gcgcggatat
    37621 ttatcgctag caccaggtat aaccaacgaa acattgccat agtttgcttt gcccccttcg
    37681 cagggggctt tttttgaaaa acggctgcgg aattagacca gttatctccc gaagaaggaa
    37741 atttccgcag cgcgtgtttt acttctgccc gtaataagcg ttaggaccat gcttacgcat
    37801 gaagtgttta ttcatcagga agctgtcgat gtgattgagt tgtggattaa tactgcgcgc
    37861 aatccacgcc attttcgcca cttcttccat cactaccgcg ttatgcaccg cgtcgtgggc
    37921 atctttcccc caggcgaacg gtccgtgctg atacaccaca attcccggcg tatgcagcgg
    37981 ctcggcgttg cccagcgttt cgataatcac tttgccagtg ttcagttcat actcaccctg
    38041 cacctcttct tcgcttaatc cacgcgtaca cgggatgtca ccaaagaagt agtcagcgtg
    38101 cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc catgcggtag catgagtgga
    38161 gtgggtatgg acaatgccgc caagcgacgg ataacgacgg tagagttcaa gatgcgtcgc
    38221 ggtgtcggaa gatgggcgat actccccttc caccacctta ccgctcatat cgaccaccac
    38281 catatcgtct gctttcatgg tttcgtaggc gacgccgctg ggcttgatca ccaccagccc
    38341 gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag gtcaccagcc cgtagcgcgg
    38401 cagatccatg ttggcttcaa atacctgctg ttttagcttt tgcattatgc cgcctccacc
    38461 atgcccgctt tcgccatgcg cgctttcacc caatcacgcg ctttcgccac ttctgccgcc
    38521 gggtcttccg ccgtttcgct ccacatctca atcaggtacg gcccgcaata gccactctgt
    38581 ttgagcgttt cgaaacaacg ttcgaaatcc actacacctt cgccaaacgg cacgtttttg
    38641 aagacgccag gtttggtgtc tttcacatgt accgcgacga tatgcccgat tccggcctgc
    38701 aactccatct gcacatcgtt gtcccacgcc gacaggttgc cgatatccgg gtagagctgg
    38761 aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct tgctgatgga gttcatcaac
    38821 ggataatcca tgatctccat cgccagcgtc acctgcgcgc ggctcgccat ctcaacgctc
    38881 tctttcaggc cgtcacggaa acgacgacgc gtttcgttat tggcttcctg atagtaaacg
    38941 tcatagcccg ccagctggat cacgcgaatg ccgacgtcct gggcgaactg gatagcttta
    39001 cgcataatct ccagcccctg cgcccgcacc gcgtcatctt cactgcccag cgggaaacga
    39061 cgatgagcag aaaggcacat ggacggcacg cgcacgccgg tttcaacaat cgcattcacc
    39121 agcgccagac gctgctcgcg gctccagtcg aggcgcgaca ggcgctcgtc cgtttcatct
    39181 accgacatct cgacaaaatc gaagcccaac gtttttgcca gttgcaggcg ttccagccag
    39241 cactccccgg cggggagcgc tttttcatag atgccaagcg ggatttgttt ggacaacata
    39301 tccgctcctt agccccacag ttcggcgatg gaacgtttga actgacgtgc agcttcaacc
    39361 ggagaagcgg catcacggat gctgcgacca gcgataaaca catgaatcgg aatacctttg
    39421 aacagcggca gatcttccag cgccaggcca ccggtgacgg tgactttgaa gcccatatcg
    39481 gaaagacgtt tgatcgcggt aatgtccgct tcgccccacg ctacgcctgc ggcctgcgcg
    39541 tcacggctgc ggtgataaac cacctgctga atgcctgcgt cacgccactg ctgcgcctgt
    39601 tcccaggtcc agtagccggt caattcgatc tgcacgtcgc cgttaaactc ttttgctacg
    39661 tccagcgcgc ctttggcggt gttgatatcc gcacagcaaa ttaccgtcac ccagtcagcg
    39721 ttggcttcga agcacatacg cgaaaggatt ttgcccgcat cagcaatttt agcgtctgcc
    39781 agtacgattt tgtgcgggta gagcgctttc aggtcacgaa ccgcacgcac gccttcgccc
    39841 acgcacagaa tggtacccac ttcgataatg tcgacttctt cggcaatcag gcgagtggtt
    39901 tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt gcaacatcgg taatgacatc
    39961 ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg agatccagta cttcctgctc
    40021 ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct tcaaacaggt tgacgatctg
    40081 catgatgccc acttcctagt gcgtgttggc atcgaccgcc gccatggtga tgaggatatc
    40141 caccgggtcg ttatcttcgt ggttgaactc cagcggcttt ttcagcgtca ccagcgcaaa
    40201 accggttttc ttaacgccct cttccgggcg accgtgcggc attgccaggc ctggtgcgag
    40261 cacaaagtac ggaccgtgct gttcaaccgc atccagaatc gcctggtagt aacgcggctc
    40321 gacaacatcc gccgccacca gcaggtcaac gccgattttt actgcgtcct gccatgtctc
    40381 agcttcagcc tgcaggcgga tggatttatt ctccgccagc gaatcacgta atttcatggc
    40441 gcgtccttac ttcacatcct gcgggaaatg cgctttgatc acttccagca gtttcgggcc
    40501 aaagtcggca ggagagagca tgttgcgcac gccaaccacg tatttgttgc cggtcacggt
    40561 gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc gcgccgctca actcgctttt
    40621 gtattcgcca accgcgcagc tatttaccgt gtggtcaatg tttgattggg ttaaaaactg
    40681 gtccactttc atcttcatga tcatggaact gccttgtccg ttgccacaca cagccagaat
    40741 acgtacggtc ataatcaaaa ctccttatta agcagactgt tctgccagtt gtttttctgc
    40801 atcttcttct gcgcgcagtg cgcggccagc gaagaacata taagccagtg caattacaat
    40861 gatgacggcc ataaaggcga taccgatgga gaagaagccc tgcatcatcg gcggtgccag
    40921 aatcgaccag tccgccatgc ccatccaggc gctcataccg gtgagtttca ccgcccagac
    40981 gcagccaaag atttcaatca tccccatcac cagacatatc ttcagcgccg cacgccagcc
    41041 gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag aagaacatcg ggataaagcc
    41101 gggaataatc aggatcgagg aaccgcaggc gaccaggatg ccaaccgcaa tcagctgacc
    41161 gatggtgccc cacataaagc cccaaaccac ggcgttcggc gcgaagctat agatagccgc
    41221 acagtcaatc gccagaaccg caccagggat caggcgctgg gagataccgt taaatgcttc
    41281 agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg atgaagatcg ccaccgcgaa
    41341 ggagaaacca gtttgcagga tgtacaccgt ccagttcact ttgcctgcca tcgcctgcac
    41401 ggtgtcgata ccgaaggaga gcagaatggc accaaagaag atggtcatca caatcgccgt
    41461 ggagacgatg ttgtcgtgga aaatattcag ccagcctggc agtttgaggt cttcaacgct
    41521 ttcctctttt ttgccgagga acggcgcaac tttataggca atccatgatg caaactgttg
    41581 ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc tgagtcggct tgtacatcat
    41641 gttggaggta atgccccagt agagcgaaac cagaatcgcc gtgcagataa tggttgtcca
    41701 catggagtag ccgaaaataa acaacgtaac ggcaatcagc cccgcctgct ggaacatgat
    41761 ataaccagtc agcatgatgg tacgaatacc ggtaatgcga cgcagcagca cgtaacagat
    41821 gttcagcgcc agcgctaaca acaccgcgta acctacccag ctataggcat cgcccatgcg
    41881 gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt gcgccgttaa tgccgtagac
    41941 ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg aggatgccgg accctgcctg
    42001 caacaacatg aaaccaatta tggttttaat cgtgccttta ataataacgc tgacactttt
    42061 gcgcagtagg atgtagccca gacaggtcac aatacccagc aacaacgggg cattggtcat
    42121 gacctggtta aaaaacacgg taaagatgtt gtagaggatc tccataacga tctcctgaag
    42181 aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta attttcaaaa
    42241 gtaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata attccacacg
    42301 gatagagtgg gattcacaca acgaaacaat tatttattta aattcattta gttaatttaa
    42361 ctacacattc cattaaatct ttccacactc aaaatcaaca acaacttccc tggcaaaaag
    42421 tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag gattaatcaa aaccaatcac
    42481 atattgattc gaattgaact atcatgaagg taaatggcga tgagtaaagt gaaaagtatc
    42541 acccgagaat cctggatcct gagcactttc ccggagtggg gtagctggct gaatgaagaa
    42601 attgaacaag agcaggtcgc ccctggcaca tttgcgatgt ggtggctagg ctgcaccggg
    42661 atctggttga aatcagaagg tggcaccaac gtttgcgttg atttctggtg cggcactggc
    42721 aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc agatgcagcg catggctggc
    42781 gtgaaaaaac tgcagccaaa cctacgcacc accccgtttg ttcttgatcc gtttgcgatt
    42841 cgccagatcg acgcggtact ggcgactcac gatcacaacg atcatatcga cgttaacgtc
    42901 gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat tcattggacc gaaaacctgc
    42961 gttgatttgt ggattggctg gggcgtaccg aaagagcgtt gcatcgtggt caaaccgggc
    43021 gatgttgtaa aagtaaaaga cattgaaatt catgcgcttg acgctttcga ccgtactgca
    43081 ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac tgccggacgg catggacgat
    43141 cgcgcggtga actatctgtt caaaacgcct ggcggctccc tgtatcacag cggcgactcc
    43201 cactactcta actattatgc gaagcacggt aacgaacatc agatcgatgt ggcgttagga
    43261 tcgtatggtg agaacccgcg cggtattacc gacaaaatga ccagcgccga tatgctgcgt
    43321 atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc accacgacat ctggtcaaac
    43381 ttccaggccg atccgcaaga gatccgcgtg ctgtgggaga tgaaaaaaga tcgtctgaag
    43441 tatggcttca agccgtttat ctggcaggtt ggcggcaaat ttacctggcc gctggataaa
    43501 gacaacttcg agtaccacta tccgcgcggt ttcgatgatt gcttcactat tgaaccggat
    43561 ctgccgttca agtcattcct gtaatctgtc gtttataccg gatagcgtgg agaagttcgc
    43621 gctatccggc gtagtatttc ctggctattt caaatatcat ctaaaaaaat caaattttat
    43681 cggaatagct catgactgaa gcacaaagac atcaaatcct cctggaaatg ctcgcacaat
    43741 tgggctttgt gaccgttgag aaagtcgttg agcgtctggg aatttcgcct gccactgcgc
    43801 gacgcgatat caataaactt gacgaaagcg gcaaactgaa aaaagtgcgc aatggcgcag
    43861 aagctattac ccagcagcgc ccgcgctgga cgccgatgaa tctgcatcag gcgcagaatc
    43921 acgatgaaaa agtacgtatc gctaaagcag cctcgcagct ggttaatccg ggcgaaagcg
    43981 tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg ggaaatgtgt ggcaagccag
    44041 tgcaaatcat cactaattat ctaccgctgg caaattacct gatcgatcaa gaacatgaca
    44101 gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc catcacttta agcccgcagg
    44161 gcagcgaaaa cagtctctat gccgggcact ggatgtttac cagcggaaaa gggctgaccg
    44221 cagaagggtt gtataaaacc gatatgctga cagcaatggc agagcagaag atgctgagcg
    44281 tggtagggaa actggtggta ctggttgata gcagtaagat tggcgaacgc gcgggaatgc
    44341 tttttagccg tgccgatcaa atcgatatgc tgatcaccgg caaaaatgct aacccggaaa
    44401 tcctgcaaca actggaagcg caaggggtca gcattctgcg tgtttaaaga tgctggcgga
    44461 aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg gtgacgcacg cctggctgcc
    44521 atgaacaggt tagcagttta tcccgtcccg tctcgcttaa ggcctgctgc aaacgtagtg
    44581 attcgtcggc aggcacaacg tcatcgtcga ggccatgcca cagcagtaga ggtctgtccc
    44641 caagttgttc caggtggttt gtcgcttccc actctgccag cggcgcgata atgttattga
    44701 attcattctg ctgtgctgcc gtttcaggta tcagcggtgg aaacagtgaa cgggcgagcg
    44761 atgtaaaata gcccgatccc atcatgctgg cggtacattt caccgtgggg tggcgagtgg
    44821 taattcccag tgccgtcatc gcgcccatcg acgcgccacc gactgccaga cggtcatcaa
    44881 gcagccagtt ttcttcggct attgccgcac gtaaagtagt gaattcctgc atactttgta
    44941 gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc gctactaaaa cggctaccgt
    45001 gatcgggcgc atccggcatg atcacccgca aaccagcctg cgccagcgca acggcaaaat
    45061 agctatacac caggctggat gaagtaaagc cgtgataaaa aattacgcac ggtaacgggg
    45121 tatctttttg cccgacagga taagcatgaa gaacgggaat atctgccagc tcgcgtgatt
    45181 ctatttcaat catctgtctc tcctttttgt ctggtatgac atgaagaaat ccttagcaca
    45241 agtttctgct ggcggtatta gttcataatg ttgagatgtg ggttacgctt tcgtcacgtt
    45301 ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca ttcccccaaa gttaaattaa
    45361 taaggcacta cactatggtt agcaggaaac gtaatagcgt tatttaccgg tttgccagtt
    45421 tattattggt gttgatgtta agtgcctgta gcgctctgca aggtacgcca cagccagcac
    45481 cgccagtaac gaaccatccg caagagattc gccgcgacca gacgcaaggg ttacagcgaa
    45541 taggtagcgt aagcacgatg gttcggggtt ctccggatga cgcattagca gaaatcagag
    45601 caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt catggtggac gaaaccatcg
    45661 tgacaggaca gtggtattca caagccattt tgtaccgtaa ataaatcaga caacttacat
    45721 agtcagcttt acattgcttt gcgtccctat gcgtttacct gtgcacaata aattaccggc
    45781 tggaagccta ggcgtttatt accgcgatgg aatgccctgc aacatgtggg gaaacgaaaa
    45841 atggagctga cgatgaaaca attacttgcc tcaccctcgc tgcaattagt gacttatcct
    45901 gcgagcgcca cggcacagtc tgcagaattc gccagtgctg actgcgtgac gggcctgaat
    45961 gaaataggtc agatctcagt tagcaatatt tcaggggatc cgcaggacgt ggaacgtatc
    46021 gtggcgttaa aggccgatga acaaggtgcg tcatggtacc gcattattac catgtacgaa
    46081 gaccagcagc ctgataactg gcgcgtacag gcgatactgt atgcatgagt gttatataca
    46141 gcaaatgaac aatgccggat gcggcgcgag cgccttgtcc ggcctacaat gttcgcatat
    46201 ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat acgcttacac acacactccc
    46261 cccgtcgccc gcagcaataa atcattctgg atctgctctg acaaccgtac gccgccgcgc
    46321 gtatctaaca tcacctgaca ccacgcctgc gccattggcg gagaggcata tttcaacatt
    46381 tgcgcaccgc agcccagcag gaatagctga tgagtaatct ctcgccccag ttcttcagct
    46441 ggcttgcgca gctgctgctg taaacgacga accgcgcgat caaaatagcg atcctgccct
    46501 ttcacttcca caaacgcttc cgacaataag tcgtatacgc ccgcttgctt attgagaacg
    46561 cgcagcacat ccaggcacat aatattgccg gaaccttccc aaatactgtt taccggcatc
    46621 tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac caatgccgcc cagcacctcc
    46681 atcgcttcgg ccacaaacgg catacctctt ttgcagatca caaattttgc cgcaggcgta
    46741 aataaacgcg cccacagggc ttctttggca tcggcacgcc ggtcccacgc tcgcgcaaga
    46801 cgaaacagca acgccgtttg cccttcaagc tgaagtgcca tgcgacttaa gacatgacgc
    46861 ataaggggct gttggatcaa tggattacca aaaacatggc gttgatgtgc atgataaatc
    46921 gccagcgaaa atgcacggcg catcatggca tggctaccca gggcgcaatc aaaacgcgtc
    46981 atcccaccca ttttcaggat cagacgaatt ccttcccctt cctgccccaa caaccagcca
    47041 atggcatcct gaaactccac ttcgcagctg gcgttagagc gattacccag cttatctttc
    47101 agccgctcga ggcgaatcgc gttgcgttgc ccgtcaggca aaaagcgcgg cacaaaaaag
    47161 caggacaaac cgcccgtagt ctgcgccagc accagatgcg catcgctttg cggcaccgag
    47221 aaaaaccatt tatgccccac cagccgataa gagccatctt ccaggcgctc tgcgcgggtg
    47281 gtgttgctca tgacatcgga accgccctgc ttttccgtca ttcccatgcc aatcaacaaa
    47341 ccgcgttttt gcccacctgg caataagtga gaatcgtagc gatcgctcag cagcggcgtg
    47401 gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca atggcgtggc ggcaaaggtc
    47461 atcgttattg gacataacga cccagcctca acctgcgcat gtaacataaa acgcgccgcg
    47521 cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg caagattgtg cacccgattg
    47581 gtacataacg cctgcatcag caggtgccag gcggggtgaa aacgcacatc gtccagacgg
    47641 cgtccttgcg catcgtagcg caataattca ggcggattca cattcgccag ccgccccagt
    47701 tcaagagatt cagccgttcc taactgctga ccaatactgg ctagaaaatc gctatcccag
    47761 ccagcacctt cacgcgttac cgcttcacag agcgcgccat cagacaggta taagttgctg
    47821 ttatttaatg gtataggttg attaaaaacg gtgtgagttt gccagtgcac tgtgtctccc
    47881 tccatcaatg gcagtcaccc ttagtatggt cactgccatg gattcctgac agaaagagat
    47941 tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa acgtgaggtg tctttggtga
    48001 tcagcgtgtt atcttcacgg atccccattc cgcacgcttc atcatcgaca atccagctac
    48061 cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg aaacgcctgg tagatcatcg
    48121 gttcatcggc gtaatcacca tcagcgtggt caacgacgtt attctgaccg tcgaaaatgg
    48181 tgacgtttcc gccttcgcgc gagtagatcg gtttacgcac atagctttcg ccagcggcaa
    48241 tctgcggttt ttcgccctca aaccaggatg caagaaggtt aggatgacca gggaagaagc
    48301 gccaaagcag cggcattagc cctttattac tcaagatact tttccataac ggctccaccc
    48361 attgctcgcg acgcttgcgc agcagcggac cgttatcgtc acgcatcatc cactccagcg
    48421 gatacagctt aaatgcacgc tggatgacat tatcatcaag atcggtcagt acaccgccga
    48481 cgcccaaacc gagatcttca atgtagataa accgcgactc ctgccctgcc tgctgggcgc
    48541 agtcctgcaa gtacagcacg gtagtcctgt cttcatcggt gtcctgacag cagcagaaat
    48601 aaaacggctc ccggctataa agctcgctaa agcgtgaaat caggcgttcc tgaatggcat
    48661 tgtactgatc ggcatcacgc ggaataatgc cgctgcgccg ggcatcctcc agccacaacc
    48721 actggaaata agccgactca tacaacgagg tcggcgtatc ggcgttgtac tccagcagct
    48781 tcaccggcgc attgccacac caggcaaaat ccatccggcc atacagcgaa ggatcgcgtg
    48841 cgcgccagct ttcggcgatc acatcccagt acaacggcgg gatcgccagt tgcgtcagga
    48901 tctcttcgtc tttaaccgcg cgatcaacca cctcaaggca catctgatgc agttccgcag
    48961 tcggtttttc gatctgctct tcaatctggc gcagagtaaa acggtaagcc cgactctcgt
    49021 cccaatagat ttcattgtcg atgatatgaa agtcgaaacc gttgttagcg gcgatctggt
    49081 ccagatcccg tcgcacagga acgttgtgtc tcagcatgat tagcctcccc aatgcccacg
    49141 ggcgctggaa gaacgaccat agccgccgcg cgaaacggtt gacgcttttt tggtggtaaa
    49201 gccgcctgaa gagtaagact cttttttgcc agaaccggag cgccaggagt aatcaccgga
    49261 agtgttgcgc cagacggggc gggaagcaaa tgaggatccg ccgctgttat agacgaacgg
    49321 ctcatcgcga tctttacgaa taacccggct taacaaaaat ccagaaacga ccgggatcca
    49381 actctgttca acattgtcgt agtagcaatt ttcgtactta gactgacaat tctgctgagt
    49441 catattcttc ggaacatcgg cataaaatgc cgctttggcg ttgttccagc cacgcgcgca
    49501 aatgtcggca ttattaccgt catcaataca atcctgcact gtcgaataaa acgttccgtc
    49561 gccgtcgtta tcgacatcgc cactatcgcc acaacctttc aatacaaaaa aagcggcacc
    49621 gcccattaga gccaacgtta agtatttttg tgcgtagcga ttgcgacgcg gttcaaaagg
    49681 attattcggt ctaccaatgc gactgatcgc tccgtgacca attttactgt tatttcttga
    49741 tttgcgtttt ctggccatga tcgtccatta ccatgtcata caagctgcgt taaaaatacc
    49801 gccagccagc gcggcggttc ccataaacat tcctgctgcg gtattgtgat taataatttt
    49861 ttcgcttaat gcgggcatat aaagtctgac accagcaaaa acgagtaact gaatcaccag
    49921 tgcaatcccg ccccaggcga aatagtctgg aatactcacc gcattgattg ccgcactgga
    49981 taatgggata acgtaaccta acaatgtacc actgaacgcc agtgaagccg cggtattatt
    50041 gtttttgatt aactgccatt cgttgtgcgg tgtaatttta gaatagataa aaagaaaaat
    50101 aatcaccata gccacgccaa taaaaaaata ggcgctaaag gcaagaagtg aatccagtat
    50161 atgcattgta aacgtccttg taattaacca ataatatgaa gtgatgtcag cgggatatcg
    50221 acacccaacg cgcgcgaaaa taaccactct ggctcgccga gatcgttaaa agattcctca
    50281 ccatttaaca gcaaatattc gtaagtatct tcggttactt gccgctggta gcccatggta
    50341 aaattatgga cctcccattt ggcatggttt tgattttcta ctttttccag catgtagacg
    50401 ggttcgatat ttcccggttc ttcgctgtta aaaaatcgct gccagcgttt ttcctgccag
    50461 tttaagatca ttgcccccat cactttggcg ttgatcgcct cgcgccagtg actctcttta
    50521 ctgataccgt agctctcttc atagacaaag agcttgatat catcaatgtc atcaatatct
    50581 tcgccgccgg tggtattgat ttgcagaaat tcatcgcctg aagtgtagta gcggaaaatc
    50641 tgactaccgc cgcccagatc gatgtggctg acagcggcta ccgtaaattc ctcacccggc
    50701 agcgcaatca gcaattcatc ttccagcaaa cgaaacgcta acgtgtcgag cgtaaagcca
    50761 ctattgagat gaagtcccag cggaccgcga gcgatagcag gcttattatc cttgccaaac
    50821 agacgctgga aaaaaccaga catattttat ccttaaaata gttgccagcc ttttagcgag
    50881 gctggcaaaa aaattactcg ccctgttggc gttgcagtct tgctaataca tcctgggcgc
    50941 tacttttatt gctaccgcca attccggctt ccgccagctt ttcatcaaga tcgcgaccgt
    51001 ctgcgacttt ttccaactgt gcagcagcat ccaggcgagc ctgacgttcg gcctgacgcg
    51061 tttgcaggcg ttttaaggat tctgccgctg tcgaaacgct ggaagatgcg ccaacggtag
    51121 aggttgttac agcctgttgt gcacgctgca tggcttcagt ggctttaacg acttccattt
    51181 gctgctcaaa ctgagcgata cgctgcgctg tcgctgtaac tgctttttcc acgccatcac
    51241 gggagacttc cagattcgac aacacttgct cttcagcggt aatgagattc tcaaggcgcg
    51301 cgatttcttc agcaacttcg ttaatcaacg acggattaac gttcttgctt aacgcttcca
    51361 gcgcacgggc ttccagactg gctttgcgct cacgtaaatc tttcagctta tcgtgactta
    51421 atttcacccg cgccagcaga tcaacgcgag attttccggc tttatcgagt tcagctttag
    51481 cgtcacgaat atgctgttcc agcatgcgca cgccctgggt ttcttcaatg gattcttccg
    51541 cctgggagat aaacgatttc cccagcgtaa ataaactttt taaaattccc ataaataccc
    51601 gtccttgtaa ttagtgtgaa tattcttctg taatttcagc cagatccaat gcgttatcta
    51661 ccagcgaggt tatctcaagc aggatatctt caagagaaga tttaagcgat aacgccccga
    51721 aaacaatgta atactcttcc tgttgcacgc tggagattcc taccgatgac aacggcatca
    51781 tcttctgatt tcttaataag aaggtattaa attcatctgg attgctgata ctacttaccg
    51841 gacaaataaa ggtttcgata atcatttggc gggaagtaaa aagaatattg atttgcaaat
    51901 cgccataatc attcatttta ataattaatg cgctctcgct atttgtcaca tcaatatttt
    51961 gttcaggtac agtttgcagc gccgtcgcca gcgccaacgg attccatgtc atatattatt
    52021 ccatatagat ttagtttaaa tattaataaa atgaatattt gcagtacgta attatcttac
    52081 cagctataga caaaaaaaaa ccatccaaat ctggatggct tttcataatt cagacgaact
    52141 agctgcgctg acgaaccgct tcaaataagc aaattccggt tgcaaccgaa acgttcaggg
    52201 aagaaacact tcctgccatt gggatgctga tcaactcatc gcaatgttca cgggtcaggc
    52261 gacgcatacc ttcaccttcc gcgcccatca ccagcgccag gcgtccggtc attttgctct
    52321 gatagagtgt atgatccgcc tcgcctgccg taccgacgat ccagatattc tcttcctgca
    52381 acatacgcat ggtgcgcgca aggttagtca cccgaatcag tggaacgctt tctgccgcgc
    52441 cgcaggctac ttttttcgcc gtagcgttga gctgtgcgga gcgatctttc ggcacaatca
    52501 ccgcgtgaac gccagcagcg tccgcgctac gcaggcacgc gccgaggttg tgcggatcgg
    52561 ttacaccgtc gaggatcagc aggaacggtt gatcgagcga agcgatcaga tccggcagat
    52621 cgttttcctg atactgacgt cctggcttca cgcgggcgat aatgccctga tgcacggcac
    52681 cgtcgctttt ctcgtcgaga tattggcggt ttgccaactg gataaccacg ccctgagact
    52741 caagggcgtg aatcagcggt aacagacgtt tatcttcacg gccttttaaa ataaagactt
    52801 cctgaaaacg ttcaggggcg cgctccagca gggcctgcac tgcgtggatg ccgtaaatca
    52861 tttcgctcat taatgtactc gttgtttacg ttttgcctga tgcactacgt ttatccggcc
    52921 tacatgatct ctgcaatata ttgaatttgc gtgattatgt aggccggata aggcgttcac
    52981 gccgcatccg gcatgaacaa agcgcatttt gtcagcaatc taaccctctt cttttaaaga
    53041 gggtattgat cactctgcca cttttttctt cgccgcacgc ttcgctttgg tcgctgcggc
    53101 tattttctgc gttttcgccg atggcttttt cgcttttctc gcgtctttct tcgccgcttt
    53161 cggcttcgtt tttttctcac cgcggaaggc gctgtctggc tcaaagttta cctttttacc
    53221 gacctgacga cgcttgccgc cttttttgcc tgcatcgcct tttttcgctt tctcgcgcgc
    53281 cgttttaccg acgttgcgcg gtgcgcgttc actggagatc agactaaagt cgattttgcg
    53341 ctcgtccata ttaaccgctt cgactcgaac ttccacgcga tcgcccaggc gataagtctg
    53401 gccgctggat tcccccatca ggcgttgccc tacctggtca aagcgatagt agtcattgtc
    53461 cagcgaagag acatggacca gaccatcaat gaacaagtcg tccagacgga cgaagaagcc
    53521 aaagccagtg acgctggaaa ttacgccttt aaagacgtta cctacctggt cgagcatgaa
    53581 gtcacacttc agccagtcag cgacatcgcg cgttgcttcg tcggcacgac gttccgccat
    53641 cgaacagtgc tgacccagtt gcaacatctc ttccatcgaa taatggtagc cgccggtttc
    53701 agtggtgttg ccctgatgcc cctgctcttt cgccagcaga tatttaatgg cgcggtgcag
    53761 cgtcaggtca ggataacgac gaatcggcga agtaaagtgc gcataggact gcaatgccag
    53821 accgaagtga ccacggtttt ctggatcgta aatcgcctgt ttcatcgagc gtagcagcat
    53881 ggtttgcagc atttctgcat caggacggtc ggcaaccgac tccagcagtt ccgcgtagtc
    53941 acgcggttcc ggcttgttac cacccggcag ctccagcccc agctccgcca gcactgaacg
    54001 gaaagaggta atcgcttcgg tgctcggctt gtcgtgaata cggaacagtg ccggttcttt
    54061 cgctttctca acgaaacgcg ccgccgagat attcgccaga atcatgcact cttcaattaa
    54121 tttgtgcgcg tcgttacgct gggtctgttc gatacgttca atacggcgtt cagcgttgaa
    54181 aatgaacttc gcttcttcgc tctcaaatga gatcccaccg cgttcttcac gggctttatc
    54241 cagcacttta tagaggttat gcaactcttc gagatgctta accagcgggg cgtactgctc
    54301 gcgcagatcc tgatcgccct gcagaatatg ccagactttg gtgtaggtca gacgcgcgtg
    54361 agagctcatc accgcttcgt agaatttgta gcccgtcagg cggcctttcg acgaaaccgt
    54421 catctcgcac accatacaca ggcggtctac ctgcgggttg agcgaacaca ggccgttaga
    54481 gagcacttcc ggcagcatcg ggataacctg cgaagggaag tacaccgacg tgccacggtt
    54541 acgcgcttct ctgtccagcg gcgttggcgg acgcacatag tagctgacgt cggcaatcgc
    54601 gacccataaa cgccagccgc cgccgcgttt tttctcgcag taaactgcat cgtcaaagtc
    54661 acgggcgtct tcgccatcaa tggtgaccag cggtaaatcg cgcagatcaa cacggcccgc
    54721 ttttgcttct tccggcactt cttctttcag cccggcaacc tgttgctcaa cagcctgcgg
    54781 ccagatgtac ggaatttcat gggtacgcag agcgatatca accgccatgc cggtgcccat
    54841 attgtcgccc agcacttcga cgattttacc caccgctttg gtgcggcgag tcggacgctg
    54901 agtcagttcg actacgacca caaagcccat ccgcgcgccc atgatctgat cgggcgggat
    54961 taagatatcg aagctcagac ggctatcgtc aggaaccaca aagccgacgc ccgcttcagt
    55021 aaagtagcga ccaacaatct ggctggtttt tggcaccagt acgcggacaa tacgcgcttc
    55081 acgacgacct ttacggtcag cgcccagcgg ctgcgccagc acctgatcgc catgaatgca
    55141 ggttttcatc tgctcgctgg agagatacaa atcatcttta cgcccttcaa cccgcagaaa
    55201 gccgtagcca tcacggtggc caataacggt acctttcacc aggtcgaggc gttccggcag
    55261 cgcatagcac tgacgacgag tgaagaccag ttgaccatcg cgctccatcg cgcgcaggcg
    55321 gcgacgcagg ccttcaagct gctcttcgcc ttcaatgtgc agttctaccg ccagctcatc
    55381 acggctggcc ggtttttcac gtttggttaa atgttcgagg ataaattccc gactagggat
    55441 gggattcgcg tatttttcag cttcgcgttc ctggaaagga tcttgtgaca tctcggttcc
    55501 tccgttgtca tctctgatga agattttcgt cactccacca gcaataattt ataaagcggt
    55561 tgattctctt caaccaaatc ggcaagcgtg tagttatcca gttccgtaag aaaactttgc
    55621 acggccttag aaagcgcctg tttcaaccga caggcaggtg taatgtggca aaactcactg
    55681 ctgcaattca ccagcgataa gggctccagc tcgcgcacca catcaccaat acgtatcgca
    55741 ctcgccggtt tacccaggcg aatgccgcca ttttttccac gaacagcagt cacgtagccg
    55801 gcacgactaa gttgattgat tattttgacc atatgattac gggagacgcc gtagacgtca
    55861 gtcacttcag aaatactggt catccgccct tctggcaatg acgccatgta gatcagcgca
    55921 cgtaatccgt aatcagtgaa actcgttaac tgcacatcaa cctcaaaagg gaaatcggga
    55981 aaaaatacat ttatattgat gataaaccag ccacaagctg tgtcgctaat ttatttcagt
    56041 tcggggaagg aaaaaagcga ggatttaaca ctgtgtcgga tagcgtaatg gcttaaccga
    56101 cacagcaatg tatcacactg ttagcccggc aggcaaaata tctgccaggc gtaccagaat
    56161 tacgcgtcga acggatcgcg cagaatcatg gtttcagtac gatccggacc ggtagagatg
    56221 atatcgatcg gcacaccagt cagctcttca atacgcttga tgtagttcag cgccgcctgc
    56281 ggcagaccgc tacgatcttt cacgccgaag gtggattcag accagcccgg catggtttcg
    56341 taaatcggct ctacaccttt ccagtcgtca gctgccagcg gagtggtcgt cacttcacga
    56401 ccatccggca tacggtaagc cacacagagt ttcacctctt tcaggccatc cagaacgtcc
    56461 agtttagtca ggcagaagcc agacagggag ttcagctgta ccgcacgacg aacggcaacg
    56521 gtgtccagcc agccggtacg acgacgacgc cccgtagttg cgccgaattc gttaccctgc
    56581 ttgcagagga actcgccagt ttcatcaaac agttcggtcg ggaacggacc tgcacctaca
    56641 cgagttgagt aagctttgag gatacccaga acataatcaa cataacgcgg gcccaggccg
    56701 gaaccggtcg ccacgccacc agcagtggtg ttggaagaag ttacgtacgg ataagtaccg
    56761 tggtcgatat ccagcagcgt accctgcgca ccttcaaaca tgacgaaatc gccacgctga
    56821 cgcgcctggt cgagcaggtc agaaacgtca accaccatag aagtcaggat gtcggcaaca
    56881 gccatcgtat catccagaac tttctggtaa tcaaccgctt cagctttgta gtagttaacc
    56941 aactggaagt tgtgatattc catcacttct ttcagttttt cagcgaaggt ttctttgtcg
    57001 aaaaggtcgc caacacgcag accgcgacgt gctactttat cttcataagc aggcccgata
    57061 ccacgaccgg tggtgccgat cgctttcgcg ccacgcgctt tctcacgcgc gttatccagc
    57121 gcaacgtgat aatcaaggat cagcggacat gcttcagaca gcagcagacg ctcacgaacg
    57181 gggatgccac ggtcttccag ttctttcatc tctttcatca gcgcggccgg agacagcaca
    57241 acaccgttac cgatgatgct ggttacattc tcgcggagaa tacctgatgg aataagatgg
    57301 agaacggttt tttcaccgtt gattacgaga gtatggcctg cgttgtgacc gccctggtag
    57361 cgtacaacat atttagcccg ttcagtcaga agatcgacga tcttaccttt accttcgtca
    57421 ccccattggg tgcccagtac gacgacgttg ttacccattt ttcaaaatca ccgtttgctt
    57481 aaaaatggat tctaccatcg ctttttcaga gttacagcac tttttgcact caaaaatgac
    57541 ctcgatcagt ctgttttttg ttcagccaat cgttttcctc aacatgtagt agaccacaac
    57601 gcccgcaacc acaagtccac cgccaaaacg acgtaaaata ttatcgggca aattggtcat
    57661 cgcagagatc atcttcttcc atgccttcgg gtaaagcatc ggccctaaac cttccagtac
    57721 caaaaccagg gcaagcgcca gccagattgt cgaattcatt cattatcctt atagaaaaag
    57781 aaaaccaccg acatccctga ggatgcggtg gctttattga cctgtaccgc agtcgttata
    57841 ttaacgcgtt gcggaagtcg gcgtcttcat gtagcggaag aaatcgctat ccgggctcat
    57901 gaccatcacg tcctgattgc cagagaagct gttctcataa gcacgcaggc tacggatgaa
    57961 tgcgtagaag tccggatctt tactgaatgc atcagcaaac agtttggctg cttcggcatc
    58021 gccttcacca cgcatgatgc ggccctgacg ctcagcttct gccagcgttc tggtcacttc
    58081 atagtcggca gtcgcgcgca gtttttccgc ttcttcctga ccttgtgaac ggtgacgacg
    58141 cgctaccgct tcacgctcgg cgcgcatacg gttgtagatc gcttcagaca cttcggtcgg
    58201 caggttgatc tgcttgatac gcacatcgac aacttcaata cccagcgccg ccatactgtt
    58261 cgggttgatg accggaactt tgcccttcgt ctcagccgtt acgcgctctg ccgcttcggc
    58321 aatggcgtta tctgctgccg gggtagtaac ttcatcttct gtacccgcag aaccggagtt
    58381 cagcgcgtca cgtacttcga gggtcagacg accacgggaa tcagtgacga tatctttcac
    58441 gtccaggcga ccaatttcag aacgcagacg gtcagagaac ttacgtttca acagcacttc
    58501 cgcttgcgaa atgtcgccac cacccgttgc caggtagtaa cggctgaaat cgctgatgcg
    58561 ccatttgatg taagaatcga cgatcaggtc tttcttctct ttggtcacaa agcggtcggc
    58621 ctggttgtcc atggtctgaa tacgtgcgtc gagcattttc accgtttcaa tgaacggtat
    58681 cttgaaatgc agacccggct cataaaccag aggtttgttg tcatcgtcac gcagtacctt
    58741 accaaaacgc agcgtaatac cgcgctcacc ttctttgacg acaaagacag acatgtaaag
    58801 cactaccagc acgatgatga taatcgcgat aactgactta cgcatcgtta ttccccctga
    58861 cgctggtagt cgttacgctg cgcgttggcg cggcgttggt ccataatatc gccctgactg
    58921 gtggacgacg tgttgcttgc tccactggtt gtggaggaag aggctggcgg cagacgcagc
    58981 agattgctgg caccgttatc gctcttcgcc gcaggggcgt taccaccttt cagcatctgg
    59041 tctaacggca gaaccatcag gtttccacct ttgtcgttaa ccagcacttt gcgggtgttg
    59101 cccaacactt tttccatcgt ctcgatatac agacgctcgc gagtaatttc cggcgcggct
    59161 ttatattccg gcagaagttt agcaaagcgc gccacttcac cctgagcttc caggatggtc
    59221 tgggccttgt acgcacgcgc ctcttcgagg atacgttgcg cctgaccgtt cgcacgcggc
    59281 tgaacttcgt tggtatacgc ttctgcttca cgaatgtatt gctgttcgtt ttcacgcgcg
    59341 gcaatcgcat cgtcaaacgc cgcttttact tcttccggcg gacgagcagc ctggaagttg
    59401 acgtccagca gcgtgatacc catgtcatac ggacgaatcg tctcttccag ttcgcgctga
    59461 gtatcgctac gaatcacggt acgaccttcc gtcagaatgc ggtccatggt gtatttaccg
    59521 ataactccac gcagggcgct gtcggtagcc tgacgcaggc tgtcatccgg gctggtcacg
    59581 ctatacagat atttttccgg attggtgacg cggtactgca cgttcatctc aacgcgcact
    59641 acgttctcgt ccgacgtcag catcacacca gaagcggcca gttcacgcac ggcttccacg
    59701 ttcaccggtt tgacttcgtc gataaacgtc ggtttccagt tcagacccgg ctcaaccaga
    59761 tggctgaatt taccaaagcg tgttaccacg ccgcgttcgg cttctttaat ggtatagaaa
    59821 ccactggccg cccagataat gacaatcgct gccgctgcga tggtaacgac acgaccgcca
    59881 agctgcgggc gcgggccttg cgatgaactg ccaccgccag atccggtgcc tttaccgccg
    59941 cccagaccac cgagcttttt gctcagtttg cggaagatat catctaaatc aggtggccct
    60001 tgatcgcgac cgcctttgtt tccatttccc tcagagttgc cgccaggttt gctgcttccc
    60061 cacgggtcgc ggtcttgtcc gttattaccg ggctgattcc acgccatgtt tgtgctccat
    60121 atttgttatg cggtgatccc tgttgtcttt cgacgccaga ggatatgact ccacgcttca
    60181 gacgctacgc cgttagatca ggtaatcgat caacgccggt tcttgtttac agaggcgacg
    60241 ccagtcaacg atcggcatac gaacttgcag acttacgctg ccgtcctcct ccatccactc
    60301 tttttctatt gcctgaagct gataaaaacg acttctcaga cgcccttcct gcggtggcag
    60361 acgcaatgta tgctgcgcca cctcgccgga aagccgcccc gtcaaagcct gaaaaagctg
    60421 tggtatcccc gctccggtct gtgcggaaag ccagacacgg atcggtttgt tctcttcgtc
    60481 ccgatcaata cgcggttcga aatcttccag catatcgatc ttgttcatca ccagcagggt
    60541 tgggatctcg tgagcgtcga tctcttcaag aaccgtattc accgcttcga tgttttcttg
    60601 tacacgcaca tccgccgcat caatgacgtg cagcagtaat gtggcttgcc gcgtctcttg
    60661 taacgtggct ttaaatgccg ccaccagatc gtgcggcagg tggcgaataa accctacggt
    60721 atctgcaagt acggtttcac cgacatctgc aacgtcaata cgccgcaacg tcgggtcgag
    60781 ggtggcaaac aactggtctg ccgcgtagac ccgcgcttcg gtgatgcgat tgaaaagggt
    60841 agatttaccg gcgttggtat atcccaccag cgaaacagta ggaacgtcgg ctttgatacg
    60901 cgattgccgc ccctgctcac gctgcttttc aactctttcc aggcgtgact gtatctgcac
    60961 gatgcgatta cgcaacaaac gacggtcggt ttcgagctgg gtttcacccg gaccacgcaa
    61021 acctatcccg cctttctgtc tttcaaggtg ggtccagcca cgcaccaggc gcgtagccag
    61081 atggcgcagc tgcgccagct caacctgcaa cttaccctca tgggtacgcg cacgttgggc
    61141 gaaaatatct aaaataaggc cggtgcggtc gataacacga cactcgcaca aacgctccag
    61201 gttacgctct tgcgccgggc tcagggcatg gtcaaaaaga acgaccgaag cacccgtcgc
    61261 tttgacagct tccgcaattt caactgcttt accttcacct acaaaatact ttgggtgcgg
    61321 cgctttacgg ctaccggtaa tcacctgcaa tgcttcgaca ccggcggaag agaccagaga
    61381 ttcaaactcc tggaggtctt ccatatcttt gtcttgcgta aaatagatgt gtaccagtac
    61441 cgcctgctca ccagcatcat aacggtcaaa caagcgtata accctctaaa tagatcagcg
    61501 gggaacgcag gatcgctggc tccccgtgta aaaaaaacag cccgaaacct tattcggttt
    61561 cttcgctgtc ctgttgcgcg gaagtattct gcgcgctgct accatgatgg tagttactgc
    61621 tggtaccgcc accggcgttg ttactgtgat gagaaaccgg gcgagacggg acaacagtag
    61681 aaatcgcgtg cttgtaaacc atctggctga ccgtgttttt caacaggatc acgaattgat
    61741 caaaagactc gatttgccct tgcagcttaa taccattcac caaataaata gaaactggaa
    61801 cacgttcccg acgcagtgcg ttcaggaacg gatcttgtaa agattgcccc ttagccattc
    61861 tctcttttcc ttatatgctt atttgtactt tgaacctttc gattctgaaa aaattgcgca
    61921 cgatacgtct caattgtaca cattcagcct gcgatagcac caacaacctg taatacttcg
    61981 tcgcgcgcct gttctggttt ttcactgtca agccagtgaa ccccttccca accacgcagc
    62041 caggttatct gccgcttcgc caactgtctc gtggcgcaaa cacctcgata aaccatttca
    62101 tcgtatgaga tttcgccttc aaggtaagac cacatctggc gataacccac gcaacgaatg
    62161 gaaggcaagt ccgtatgcaa atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa
    62221 cctgaagcca acatctgatg aaaacgctgc tcaatgcgtt gatggagcag ttcacggctc
    62281 gccggggcga tggcgaactg atgcacctga tacggtagag cgtctcctga cgtttgcgtc
    62341 agttccgtta aagttttacc cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt
    62401 ggatcatttg gatgaatcct tgctgccgca accggatcta cctcctgaag ttgacgatgc
    62461 aatgactccc aaccttgctc taccgcctgt tgctcaattc tggcccgcac ttccgggtct
    62521 gccgacggta gcggcgacaa cccttccagc aatgccttga aatacaacat cgtaccgccc
    62581 actaacagtg ggatccgccc cgccgcggtg atatcggcca tttccgccag cgcatcgcgg
    62641 cgaaaatcag cagccgagta agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc
    62701 gcggcgagta actcttcagc gttcggcttc gccgtcccga tatccatccc tttgtaaata
    62761 agggcagaat caacgcttat caactctact ggtaaaattt tacgcagctc aatggctaac
    62821 gccgttttac cggaggccgt cggccccatc aaaaaaatcg ccttaggcag gctcgcctta
    62881 ctgatatcac tcatctttca gggcttttat cgccggatgt aaatcaacag attgtaacag
    62941 accacccggc ggcgttttca caagttgcgg acataaccgt tccacgtccg ccagcagggt
    63001 tatggcctgt gccattgacc actgcgcatg ttcgctcatc agatttcgtg caatccactg
    63061 cgcaatattg ccaggttcga atacggactg cttcgccagg tagcctatca gttcaggaat
    63121 caagatttgt aaattttgtt ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg
    63181 tgcatctgac tggaaatcaa tacccaattc cgccagggca gactgcgctt tttctaatgc
    63241 cgatttttct tcgccagaaa cttttagccg caacggaatc agcaacggct gggcgcaaac
    63301 tggcgcttca cccggcgtca gttgcgcctg acgcagccaa cgttctgcca ctggcaaggc
    63361 taaaagtgaa atgttgccat cgcgctccag caacgcacag tcggaatgga cgatagtcag
    63421 tacccgacca aaactctgac tgttcgccgc aagtgcaggt tcctgcagtt ccggcgcttt
    63481 tggtttttgc atcggcgcgg gcgtttgcaa aagctggcga tacacttcac cttgctgttt
    63541 ctggtagcct ggctgcgcat tcggccaggg ggcagccgga cgactgcctg aggcgggcgc
    63601 aggagagtag cgcggagcta ccggctcacg agctgccggt tctgcaaagt gattgcgccc
    63661 cgccgccacg cggttttccg gaatgggacg cggtgcaggt tggggttcat cgtccagcgg
    63721 tagcggcgtt tccagttgct gttgtagcac gctcagcacg ccctgataga taaaatcatg
    63781 caccagacgc gactgatgga aacgcacttc gtgtttggcg gggtgtacgt tgacgtccac
    63841 ctgatgcggg tcgatctcca gatacaacac aaacgccggt tgttgatcgg cccccagttt
    63901 gtcttcgcag gcctggcgga tcgcatgatt gatcaggcga tcgcgcatca tgcgaccatt
    63961 cacgtagcaa tactgaattt ccgccagtgc gggcgtagtg tgatttggat cggccaccca
    64021 gccgcgcagc gtgagatcgc cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc
    64081 ggtgccacaa atcgcgccta agcgccgttc tttttgcccg ccttccggca ctgcgcggta
    64141 ctgacgcaca attttaccgt tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc
    64201 aatgcggcgg atgatttcat caatatggct aaattcggtt ttctcggtgc gcaggaattt
    64261 gcgccgcgcc ggggtgttgt agaacagatc cagcacctcc agcgtcgtcc ccacaggatg
    64321 cgccgccggt tttaccgtca cgttcatatc gcgcccttcg gcataggcct gccaggcttc
    64381 ctgctgttct gcggtgcgtg aagtgagcgt caggcgggaa accgaactga tactcgccag
    64441 cgcctcaccg cgaaagccca ggctgataat ggcttcgaga tcgtccagag aggcgatttt
    64501 actggtggca tgacgagcca gcgccagcgc cagctcatct tttttgatac cgcagccgtt
    64561 atcacgaatg cggataagtt tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc
    64621 acctgcatcg aggctgtttt ccaccagttc tttgactacc gacgcaggtc gctcgaccac
    64681 ctcacctgcg gcaatctggt tcgccagttg tggcggtaag acctgaattg gcatcaatcg
    64741 tccttagttt ggcagcgtgc gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc
    64801 cccctgcggc gcagattgca tcggatgcgc aaggaaataa ttgcgcaggc ctttgtaaat
    64861 ggcttctgcc agctgttgtt ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt
    64921 gctgataaaa ccggtttcga ccagtactga cgggatatcc ggcgaacgca gaacgccgag
    64981 actcgcgtgt tctggtcggc gtttatgtat ttcgccaatg cgttgcaact gactgatcat
    65041 actggtcgct acatcatacc ctacccgttg ggaatgaccg aactgtaaat ccagcaccgc
    65101 ctggcttaaa taggggtcag actgactgtt cgccagcaca tcacccgccc cacccagcag
    65161 ctccgactgt ttctcgtgct gctccagcca gctggccatt tcgctgttgg cgcgacggtt
    65221 agagagcacc catacggaag cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat
    65281 cgacacgagg aaattggcgt tttgcttacg tgccacatca ctgcgcccca tcaccgagat
    65341 aaagtaatcc ccgtcacggg ttaaaacgcc cttaaacatc ggatcgtcat tgagcaaagt
    65401 acgcaattta cgcgcgatgg cgatggtgac atttttctcc cgcgtaccac cggggccgat
    65461 agcgccaggg tcctgaccgc cgtgtccggc atcaatagcg atgataattt tatcgccagt
    65521 gttagccgtc gcgcgcgctg ccggacgcgt taccgtatta ctgctgataa cacccgtagt
    65581 gcggttactt tccgttttaa acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac
    65641 cgcaggcgtt tcaacgcgtt tcgcaaccac gggcggcgga ggaggaggtg gcggcgcatc
    65701 ggcgttaatc gtaaagacga cagtgtaatt gctgccattc tgccgcttca ccgcttcggt
    65761 tttaccgttt tcggtaagat cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc
    65821 agagcgaatc gccttcacca gattattgcc gctgaacaac aacggcagtc cctgaatcac
    65881 gcccgtttgt ttgatatcga gcgccacggt gcgtttgctt tgatggctaa acgcataatc
    65941 aggatcgcca ataaaactca acgttatccg cgcctgttga ttaccgttag aaacctgaat
    66001 atcagagagc gtcgcggcac ccaccggcgt gcacagcagc agcagcgtcg ctaccaacca
    66061 atttctgatg cgatacatca tcccgccacc tttcaaaggt taaccggcta aacgcgccag
    66121 caacaattca cccgcagagg aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata
    66181 atcaatgtgt atttcgacat ccgggtcagg aagaacacct gtaccttgtt gtggccactc
    66241 caccaggcag atggcatcgt tggcaaaata atcgcggatc cccataaact ccagctcctc
    66301 gggatcggca aggcggtaca aatcaaagtg ataaaccatt aaattgtcga gcgtataggg
    66361 ttcgaccagc gtataagtgg ggcttttgac attaccctga tgacccagag cttgtaaaaa
    66421 gccccggcta aaggtggttt tacctgcgcc taaatcgcca tacagataga ttacggttgc
    66481 gccatcgcag gctttcgcta cccgctcgcc caggtctaat gttgcctgct catcagggag
    66541 cggaattact cgattcatca tggtttttat cagtcacttc cgggttaaca atacgctgta
    66601 gcgtggaaaa gagatcggtt gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt
    66661 cagctgcagc accgtgcgcg acacagcccg cacaggcggc atcatacggc gacagttttt
    66721 gcccaagcaa tgcgccaata ataccagaga gcacatcgcc catgccgccg ctcgccatgc
    66781 ctgcatttcc ggcatcaata atgcctaaag cgtcaggatg ggcggcgacc acggttccgg
    66841 cacctttcag caccgctacg ccgccataac gttgtaccag acgtttggcg caatgtaagc
    66901 ggtcactttc aatttcagcg acggaacagc ctaacaaccg tgcggcctcg ccaggatgcg
    66961 gcgtgatcac gcgattgtga cgcttatcgg gattgattgc cagcaggttc aatgcatcgg
    67021 catcccacaa catcggtttg cgaaaattct caactttttg cagtgctttt ttcccccact
    67081 cttgctggcc cagaccggga ccaatcacca ccacatcggc ccattccagg ctttcggtaa
    67141 gagagtccat cgtcagttca tgcaccatca attccggtcg tgcagtcagc agcggcgcaa
    67201 tgttttcact gcgggtcagt actcggacta aaccagcacc agcacgtagc gccgcttccc
    67261 ccgtcatacg aatagccccc gccgtgccgt gatcgccacc gataattacc agccgcccgt
    67321 gatcgccttt atgcgaagtc gggcgacgcg gttttagcca gtgagaaagt tgttctgccg
    67381 aaaaccgctg aattttcgtc tcctgacctg ccagccaact atccagcccc agtgagtcaa
    67441 aatgcagttg tccggtaaca tcccgcgctt ttccagtgag caagcctggt ttcagcgcaa
    67501 taaaagtgat ggtgtgatcg gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca
    67561 gcaggccgga agggatatca accgccgcaa tcggcgcagg atgggaatta gcgtggtcga
    67621 ttaactggct aatggattcg cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa
    67681 caatcagatc taccgattcg ggccagacaa tattcgaagc atggatctcg cctcccgcgt
    67741 ttaaccatgc ttcgcgtgcc agcgcggcct cttccggcaa cggtttgtcg ctctcctggg
    67801 ccaacaacgt gacctcaatg ccgaccgctg tggccagtcg cgcgaccacg tagccatcgc
    67861 cgccgttatt accatgaccg cacagcacca gccagtggcg ggcgtcagga tacgccgaac
    67921 gacacacctg gaatgcggct tcgccagcgc gaagcatcag ctcatagagt gtaagcccca
    67981 gcgcatctgc cgcctcacgt tctccgcggc ggatatcgtc ggcgtgccag acggtgtgtg
    68041 gtatacttac ggggtttttc ttcattgtat ggtccgtcat gtcagagccc ctcgatctca
    68101 atcagttagc gcaaaaaatt aaacagtggg ggctggaact gggctttcag caggtaggta
    68161 ttaccgatac cgatctcagc gagtccgagc ccaaactgca agcatggctg gacaaacaat
    68221 accacggcga aatggactgg atggcacgtc acggtatgct gcgcgctcgc ccccatgagt
    68281 tattacccgg tacgctgcgc gtgatcagcg tgcggatgaa ttaccttcct gctaacgccg
    68341 catttgccag cacgctgaaa aaccccaaac tcggctatgt tagccgttat gcgctgggcc
    68401 gtgactatca caaacttctg cgcaaccgac tcaaaaagct gggcgagatg attcagcaac
    68461 attgtgtttc gctgaatttt agaccgtttg tcgattctgc gcctattctc gagcgcccgt
    68521 tagctgcaaa agctgggctc ggctggacag gtaagcactc acttatcctc aatcgcgagg
    68581 ccggttcttt cttcttttta ggcgaattgc tggtcgatat tccgctgccc gtggatcaac
    68641 cagtcgagga aggatgcggt aaatgcatag cctgtatgac gatttgcccg accggtgcca
    68701 tcgtcgagcc atataccgtc gatgctcgcc gctgtatctc ttatctcacc atcgaactgg
    68761 aaggggcgat cccggaagag ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg
    68821 actgccagct tatctgcccg tggaatcgct attcgcaact cactacagaa gacgatttca
    68881 gcccgcgtaa gccgctacac gcaccggaac tcattgagtt attcgcctgg agcgaagaga
    68941 agtttttaaa agtcacggaa ggatcggcga ttcgccgtat aggtcacctg cgctggctgc
    69001 gtaatatcgc cgtggcatta ggcaatgccc cctgggatga aacgattctg gcggcgctgg
    69061 agagtcgcaa aggtgagcac ccacttcttg atgagcacat agcgtgggcg atggcgcagc
    69121 aaatcgagag gcgaaatgcg tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg
    69181 ttcgggtgat tgaaaaaggg ttaccacgtg acgcctgatt catccacagc ttgtgaataa
    69241 aaataaaaac gcattgcaat tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa
    69301 cgaaatctta atttttaact tatctatcaa taagttaaaa aaatactatt caccgtgcga
    69361 agtttctttg aaattaagac tacagcctga ggctgtggat aactctgttt acaagagttt
    69421 gtcagagaca acaaaaacca gcaataacgc atcgcgtcgc tgtggatatt ttattgagag
    69481 aaga
//
LOCUS       DAZFJF010000010        67710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFJF010000010 DAZFJF010000000
VERSION     DAZFJF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.010"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1753
                     /gene="adeD"
                     /locus_tag="ACHKAU_000768"
     CDS             <1..1753
                     /gene="adeD"
                     /locus_tag="ACHKAU_000768"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO5529885.1"
                     /translation="NHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEIS
                     GPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETAT
                     LPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGAS
                     FTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKEL
                     NAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCML
                     CTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGK
                     QADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASD
                     FALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACG
                     LLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSH
                     LPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQ
                     GLFDGEKFAFTTLEVTE"
     gene            complement(1798..3189)
                     /gene="uhpT"
                     /locus_tag="ACHKAU_000769"
     CDS             complement(1798..3189)
                     /gene="uhpT"
                     /locus_tag="ACHKAU_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO5529886.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3445..4764)
                     /gene="uhpC"
                     /locus_tag="ACHKAU_000770"
     CDS             complement(3445..4764)
                     /gene="uhpC"
                     /locus_tag="ACHKAU_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5529887.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            complement(4774..6276)
                     /gene="uhpB"
                     /locus_tag="ACHKAU_000771"
     CDS             complement(4774..6276)
                     /gene="uhpB"
                     /locus_tag="ACHKAU_000771"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO5529888.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6276..6797)
                     /gene="uhpA"
                     /locus_tag="ACHKAU_000772"
     CDS             complement(6276..6797)
                     /gene="uhpA"
                     /locus_tag="ACHKAU_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO5529889.1"
                     /translation="MITVALIDDHLIVRSGFAQLPGRGVQVCICDISMPDISGLELLS
                     QLPKGMATIMLSVHDSPALVEQALNAGARGFLSKRCSPDELIAAVHTVATGGCYLTPD
                     IAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAAELGLSPKTVHVHRANLMEKLGVS
                     NDVELARRMFDGW"
     gene            complement(6872..7162)
                     /gene="ilvN"
                     /locus_tag="ACHKAU_000773"
     CDS             complement(6872..7162)
                     /gene="ilvN"
                     /locus_tag="ACHKAU_000773"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5529890.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7166..8854)
                     /gene="ilvB"
                     /locus_tag="ACHKAU_000774"
     CDS             complement(7166..8854)
                     /gene="ilvB"
                     /locus_tag="ACHKAU_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO5529891.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8960..9058)
                     /gene="ivbL"
                     /locus_tag="ACHKAU_000775"
     CDS             complement(8960..9058)
                     /gene="ivbL"
                     /locus_tag="ACHKAU_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO5529892.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9623..9712
                     /gene="tisB"
                     /locus_tag="ACHKAU_000776"
     CDS             9623..9712
                     /gene="tisB"
                     /locus_tag="ACHKAU_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO5529893.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9975..10049)
                     /gene="ysdE"
                     /locus_tag="ACHKAU_000777"
     CDS             complement(9975..10049)
                     /gene="ysdE"
                     /locus_tag="ACHKAU_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO5529894.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10131..11315
                     /gene="emrD"
                     /locus_tag="ACHKAU_000778"
     CDS             10131..11315
                     /gene="emrD"
                     /locus_tag="ACHKAU_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO5529895.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11323..11820)
                     /gene="yidF"
                     /locus_tag="ACHKAU_000779"
     CDS             complement(11323..11820)
                     /gene="yidF"
                     /locus_tag="ACHKAU_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5529896.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11817..12179)
                     /gene="yidG"
                     /locus_tag="ACHKAU_000780"
     CDS             complement(11817..12179)
                     /gene="yidG"
                     /locus_tag="ACHKAU_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO5529897.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12169..12516)
                     /gene="yidH"
                     /locus_tag="ACHKAU_000781"
     CDS             complement(12169..12516)
                     /gene="yidH"
                     /locus_tag="ACHKAU_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO5529898.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12626..13075
                     /gene="yidI"
                     /locus_tag="ACHKAU_000782"
     CDS             12626..13075
                     /gene="yidI"
                     /locus_tag="ACHKAU_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO5529899.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13122..14615)
                     /gene="yidJ"
                     /locus_tag="ACHKAU_000783"
     CDS             complement(13122..14615)
                     /gene="yidJ"
                     /locus_tag="ACHKAU_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5529900.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGNPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14612..16327)
                     /gene="yidK"
                     /locus_tag="ACHKAU_000784"
     CDS             complement(14612..16327)
                     /gene="yidK"
                     /locus_tag="ACHKAU_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO5529901.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            16494..17387
                     /gene="yidL"
                     /locus_tag="ACHKAU_000785"
     CDS             16494..17387
                     /gene="yidL"
                     /locus_tag="ACHKAU_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5529902.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17384..18198)
                     /locus_tag="ACHKAU_000786"
                     /pseudo
     CDS             complement(17384..18198)
                     /locus_tag="ACHKAU_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18198..19814)
                     /locus_tag="ACHKAU_000787"
     CDS             complement(18198..19814)
                     /locus_tag="ACHKAU_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5529903.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            20110..20826
                     /gene="yidP"
                     /locus_tag="ACHKAU_000788"
     CDS             20110..20826
                     /gene="yidP"
                     /locus_tag="ACHKAU_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5529904.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20823..22484)
                     /gene="yidE"
                     /locus_tag="ACHKAU_000789"
     CDS             complement(20823..22484)
                     /gene="yidE"
                     /locus_tag="ACHKAU_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO5529905.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22681..23109)
                     /gene="ibpB"
                     /locus_tag="ACHKAU_000790"
     CDS             complement(22681..23109)
                     /gene="ibpB"
                     /locus_tag="ACHKAU_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO5529906.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23221..23634)
                     /gene="ibpA"
                     /locus_tag="ACHKAU_000791"
     CDS             complement(23221..23634)
                     /gene="ibpA"
                     /locus_tag="ACHKAU_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO5529907.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23628..23700)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23940..24272
                     /gene="yidQ"
                     /locus_tag="ACHKAU_000792"
     CDS             23940..24272
                     /gene="yidQ"
                     /locus_tag="ACHKAU_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5529908.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24274..25488)
                     /gene="yidR"
                     /locus_tag="ACHKAU_000793"
     CDS             complement(24274..25488)
                     /gene="yidR"
                     /locus_tag="ACHKAU_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO5529909.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25589..26653
                     /gene="cbrA"
                     /locus_tag="ACHKAU_000794"
     CDS             25589..26653
                     /gene="cbrA"
                     /locus_tag="ACHKAU_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO5529910.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            complement(26650..27987)
                     /gene="dgoT"
                     /locus_tag="ACHKAU_000795"
     CDS             complement(26650..27987)
                     /gene="dgoT"
                     /locus_tag="ACHKAU_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5529911.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(28062..29210)
                     /gene="dgoD"
                     /locus_tag="ACHKAU_000796"
     CDS             complement(28062..29210)
                     /gene="dgoD"
                     /locus_tag="ACHKAU_000796"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO5529912.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29207..29824)
                     /gene="dgoA"
                     /locus_tag="ACHKAU_000797"
     CDS             complement(29207..29824)
                     /gene="dgoA"
                     /locus_tag="ACHKAU_000797"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO5529913.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29808..30686)
                     /gene="dgoK"
                     /locus_tag="ACHKAU_000798"
     CDS             complement(29808..30686)
                     /gene="dgoK"
                     /locus_tag="ACHKAU_000798"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO5529914.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30683..31372)
                     /gene="dgoR"
                     /locus_tag="ACHKAU_000799"
     CDS             complement(30683..31372)
                     /gene="dgoR"
                     /locus_tag="ACHKAU_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO5529915.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31650..32306
                     /gene="yidX"
                     /locus_tag="ACHKAU_000800"
     CDS             31650..32306
                     /gene="yidX"
                     /locus_tag="ACHKAU_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO5529916.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32352..33164)
                     /gene="yidA"
                     /locus_tag="ACHKAU_000801"
     CDS             complement(32352..33164)
                     /gene="yidA"
                     /locus_tag="ACHKAU_000801"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO5529917.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33279..33677)
                     /gene="yidB"
                     /locus_tag="ACHKAU_000802"
     CDS             complement(33279..33677)
                     /gene="yidB"
                     /locus_tag="ACHKAU_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO5529918.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33917..36331)
                     /gene="gyrB"
                     /locus_tag="ACHKAU_000803"
     CDS             complement(33917..36331)
                     /gene="gyrB"
                     /locus_tag="ACHKAU_000803"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO5529919.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36360..37433)
                     /gene="recF"
                     /locus_tag="ACHKAU_000804"
     CDS             complement(36360..37433)
                     /gene="recF"
                     /locus_tag="ACHKAU_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO5529920.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37433..38533)
                     /gene="dnaN"
                     /locus_tag="ACHKAU_000805"
     CDS             complement(37433..38533)
                     /gene="dnaN"
                     /locus_tag="ACHKAU_000805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO5529921.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38538..39941)
                     /gene="dnaA"
                     /locus_tag="ACHKAU_000806"
     CDS             complement(38538..39941)
                     /gene="dnaA"
                     /locus_tag="ACHKAU_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO5529922.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40238..40318
                     /locus_tag="ACHKAU_000807"
     CDS             40238..40318
                     /locus_tag="ACHKAU_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529923.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40548..40688
                     /gene="rpmH"
                     /locus_tag="ACHKAU_000808"
     CDS             40548..40688
                     /gene="rpmH"
                     /locus_tag="ACHKAU_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO5529924.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40705..41064
                     /gene="rnpA"
                     /locus_tag="ACHKAU_000809"
     CDS             40705..41064
                     /gene="rnpA"
                     /locus_tag="ACHKAU_000809"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO5529925.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            41028..41285
                     /gene="yidD"
                     /locus_tag="ACHKAU_000810"
     CDS             41028..41285
                     /gene="yidD"
                     /locus_tag="ACHKAU_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO5529926.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41288..42934
                     /gene="yidC"
                     /locus_tag="ACHKAU_000811"
     CDS             41288..42934
                     /gene="yidC"
                     /locus_tag="ACHKAU_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO5529927.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            43040..44404
                     /gene="mnmE"
                     /locus_tag="ACHKAU_000812"
     CDS             43040..44404
                     /gene="mnmE"
                     /locus_tag="ACHKAU_000812"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO5529928.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            44647..44721
                     /gene="tnaC"
                     /locus_tag="ACHKAU_000813"
     CDS             44647..44721
                     /gene="tnaC"
                     /locus_tag="ACHKAU_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO5529929.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            44942..46357
                     /gene="tnaA"
                     /locus_tag="ACHKAU_000814"
     CDS             44942..46357
                     /gene="tnaA"
                     /locus_tag="ACHKAU_000814"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO5529930.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            46448..47695
                     /gene="tnaB"
                     /locus_tag="ACHKAU_000815"
     CDS             46448..47695
                     /gene="tnaB"
                     /locus_tag="ACHKAU_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO5529931.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFHVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            47827..49002
                     /gene="mdtL"
                     /locus_tag="ACHKAU_000816"
     CDS             47827..49002
                     /gene="mdtL"
                     /locus_tag="ACHKAU_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO5529932.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            48977..49936
                     /gene="yidZ"
                     /locus_tag="ACHKAU_000817"
     CDS             48977..49936
                     /gene="yidZ"
                     /locus_tag="ACHKAU_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO5529933.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            50093..50842
                     /gene="yieE"
                     /locus_tag="ACHKAU_000818"
     CDS             50093..50842
                     /gene="yieE"
                     /locus_tag="ACHKAU_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO5529934.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            50864..51430
                     /gene="yieF"
                     /locus_tag="ACHKAU_000819"
                     /gene_synonym="chrR"
     CDS             50864..51430
                     /gene="yieF"
                     /locus_tag="ACHKAU_000819"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO5529935.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(51484..52821)
                     /gene="adeP"
                     /locus_tag="ACHKAU_000820"
     CDS             complement(51484..52821)
                     /gene="adeP"
                     /locus_tag="ACHKAU_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO5529936.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            52988..53653
                     /gene="yieH"
                     /locus_tag="ACHKAU_000821"
     CDS             52988..53653
                     /gene="yieH"
                     /locus_tag="ACHKAU_000821"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO5529937.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            53720..54193
                     /locus_tag="ACHKAU_000822"
     CDS             53720..54193
                     /locus_tag="ACHKAU_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5529938.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            54242..54829
                     /gene="cbrC"
                     /locus_tag="ACHKAU_000823"
     CDS             54242..54829
                     /gene="cbrC"
                     /locus_tag="ACHKAU_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO5529939.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(54891..55613)
                     /gene="yieK"
                     /locus_tag="ACHKAU_000824"
     CDS             complement(54891..55613)
                     /gene="yieK"
                     /locus_tag="ACHKAU_000824"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5529940.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(55628..56797)
                     /gene="yieL"
                     /locus_tag="ACHKAU_000825"
     CDS             complement(55628..56797)
                     /gene="yieL"
                     /locus_tag="ACHKAU_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5529941.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(56824..58440)
                     /gene="bglH"
                     /locus_tag="ACHKAU_000826"
     CDS             complement(56824..58440)
                     /gene="bglH"
                     /locus_tag="ACHKAU_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO5529942.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(58526..59920)
                     /gene="bglB"
                     /locus_tag="ACHKAU_000827"
     CDS             complement(58526..59920)
                     /gene="bglB"
                     /locus_tag="ACHKAU_000827"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO5529943.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(59939..61816)
                     /gene="bglF"
                     /locus_tag="ACHKAU_000828"
     CDS             complement(59939..61816)
                     /gene="bglF"
                     /locus_tag="ACHKAU_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO5529944.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(61950..62786)
                     /gene="bglG"
                     /locus_tag="ACHKAU_000829"
     CDS             complement(61950..62786)
                     /gene="bglG"
                     /locus_tag="ACHKAU_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO5529945.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(63072..63797)
                     /gene="phoU"
                     /locus_tag="ACHKAU_000830"
     CDS             complement(63072..63797)
                     /gene="phoU"
                     /locus_tag="ACHKAU_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO5529946.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(63812..64585)
                     /gene="pstB"
                     /locus_tag="ACHKAU_000831"
     CDS             complement(63812..64585)
                     /gene="pstB"
                     /locus_tag="ACHKAU_000831"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO5529947.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(64676..65566)
                     /gene="pstA"
                     /locus_tag="ACHKAU_000832"
     CDS             complement(64676..65566)
                     /gene="pstA"
                     /locus_tag="ACHKAU_000832"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO5529948.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(65566..66525)
                     /gene="pstC"
                     /locus_tag="ACHKAU_000833"
     CDS             complement(65566..66525)
                     /gene="pstC"
                     /locus_tag="ACHKAU_000833"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO5529949.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(66612..67652)
                     /gene="pstS"
                     /locus_tag="ACHKAU_000834"
     CDS             complement(66612..67652)
                     /gene="pstS"
                     /locus_tag="ACHKAU_000834"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO5529950.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
BASE COUNT        16541 a        17982 c        16971 g        16216 t
ORIGIN      
        1 taaccataaa tttcatcaca ttagccgggc tgaataccag gaattgttag ccgtttcccg
       61 tggcgacgct gttgccgatt atattattga taatgtctct attctcgacc tgatcaatgg
      121 cggagaaatt tccggcccaa ttgtgattaa aggacgttac attgccggtg ttggcgcaga
      181 atacgctgat gctccggctt tgcagcggat tgatgctcgc ggcgcaacgg cggtgccagg
      241 gtttattgat gctcacctgc atattgaatc cagcatgatg acgccggtca cttttgaaac
      301 cgctaccctg ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa tcgtcaacgt
      361 gatgggagaa gccggattcg cctggtttgc ccgctgtgcc gaacaggcaa ggcaaaacca
      421 gtacttacag gtcagctctt gcgtacccgc cctggaaggc tgcgatgtta acggtgccag
      481 ttttaccctt gaacagatgc tcgcctggcg ggaccatccg caggttaccg gccttgcaga
      541 aatgatggac taccctggcg taattagcgg gcagaatgcg ctgctcgata aactggatgc
      601 atttcgccac ctgacgctgg acggtcactg cccgggtttg ggtggtaaag aacttaacgc
      661 ctatattact gcgggtattg aaaactgcca cgaaagttat cagctggaag aaggacgccg
      721 gaaattacaa ctcggcatgt cgttgatgat ccgcgaaggg tccgctgccc gcaatctcaa
      781 cgcgctggca ccgttgatca acgaatttaa cagcccgcaa tgcatgctct gtaccgatga
      841 ccgtaacccg tgggagatcg cccatgaagg acacatcgat gccttaattc gccgcctgat
      901 cgaacaacac aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt cgacggcgcg
      961 ccactttggt ctgaatcacc tcggcttact ggcacccggc aagcaggccg atatcgtcct
     1021 gttgagcgat gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg agccgattga
     1081 tgcgcaaacc ttacaggcgg aagagtcggc gagactggca caatccgctc cgccatatgg
     1141 caacaccatt gcccgccagc cagtttccgc cagcgacttt gccctgcaat ttacgcccgg
     1201 aaaacgctat cgggtcattg acgtcatcca taacgaattg attacgcact cccactccag
     1261 cgtctacagc gaaaatggtt ttgatcgcga tgatgtgagc tttattgccg tacttgagcg
     1321 ttacgggcaa cggctggctc cggcttgtgg tttgcttggc ggctttggac tgaatgaagg
     1381 tgcgctggct gcgacggtca gccatgacag ccataatatt gtggtgatcg gtcgcagtgc
     1441 cgaagagatg gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc tgtgcgtggt
     1501 acgtaacggc caggtccaaa gtcatctgcc gttgcccatt gccggcctga tgagcaccga
     1561 cacggcgcag tcgctggcgg aacaaattga cgccttgaaa gccgccgccc gtgaatgcgg
     1621 tccgttaccc gatgagccgt ttattcagat ggcgtttctt tctctgccag tgatccccgc
     1681 gctaaaacta accagtcagg ggctatttga tggcgagaag tttgccttca ctacgctgga
     1741 agtcacggaa taataaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta
     1801 tgccactgtc aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccatcaccgc
     1861 cactatcgcc atcaggcaga tacaaccaat cgctgcgata tcaagtgcgg cgaaggtgcc
     1921 tgcccagccg gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt
     1981 ggcaaagctg tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat
     2041 cgctttttta ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac
     2101 caggaaaccc aacgcaaaga gagaagccag atagatatat tcgttgctgg catgttgata
     2161 cacaccgagc gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc
     2221 gttcgccagg tcagagagcc agccccacag cagcgtaccg accagtgcac cagcttcaaa
     2281 cagcgtaaag ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac
     2341 ggttgaccac tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag
     2401 cagccagatc actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc
     2461 ggtagattct gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt
     2521 gccgaggcca taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat
     2581 cagcgcgata atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt
     2641 tgccccgaac agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca
     2701 gaaaccgagg aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact
     2761 gcacgaaccg ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa
     2821 caggctaacc gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagacag
     2881 gatcagcatg aacggcagga attgtttggt gtttttgccg tcggcgtagt aggaaaccag
     2941 cgttttaccc acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat
     3001 gctcaacccg taggtcgaga tcatgtcgtt ctgcgcgatg ttaaagttct tgcgaatcag
     3061 gtacatcgtc aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca
     3121 cattttgcgc cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaagaa
     3181 agccagcatg ggttactcct gatttccctt catacttcaa gccgcaggtg ccttagctgc
     3241 acgcattcct cccggtcaca tagttcacta tgctcccggg actcacgcgt ttgctgcgtt
     3301 cctgcaactt gaattatttt gggaaagaat taatgaatga tggcattgta aaaatcagca
     3361 gcaaatcagg cctgagatag cgtccaggca aaacctggaa aatttcttag ttttgccgga
     3421 tatgaagtga aaaggtgaga tgcatcacgc ttcgcgcggt gcctgggcgt tcaaaaaggg
     3481 cagtaacagc agtgcggaaa ccccggcggc gatagagata accacaaaaa atccgctcca
     3541 gtgccaggta tcgagtactt tcgccagcgg ccaaccagca agcgacgccc ccagataagc
     3601 aaacaagccg acaaaccccg tcgccgcccc tgcagcctct ttgtgggaac actctgccgc
     3661 cgccatgccg attaacatct gcgggccaaa gacaaaaaaa ccaatggtga agaagcaggt
     3721 tgcctgcatc acgtagctgg caaatggcat cagccacagc gagccgactg aaagcaaaat
     3781 tccggcggcg aaaatcaaat tcatcggccc acggttgccg ttaaacaatt tgtccgagcc
     3841 ccaaccggct accagcgcac cgataaatcc gcccagttca aacatcgtca ctgccgtatt
     3901 cgccgtgacc agatcgacgc ccagcgtctc ggacatatac aaattgcccc agtcgttgat
     3961 tgccgcccgg accacataga ccagcacata gcaaaacgaa agcagccaga tatacggatt
     4021 cagcaacaca tatttggtca ggatctcttt acgcgtcaac cctgcccctt cttgttgttg
     4081 agcaatttcc agcgcgtcgt gccgccattc accgaccgcc ggtaaaccta acgcctgcgg
     4141 gcgatcgcgt agccgccagc agagaaaaat ccccacgact atcgccatac aaccagcaat
     4201 catcatcccg gcacgccagc cgtaatgcag cgcgctcgct gccatcacaa tgggaatgag
     4261 tgcgccgccg acgttatgcg ccgtgttcca taatgcccac caaccgccgc gctcggtacg
     4321 tgaataccag gccgttaaca gacgcgcaca caccggtgaa ccccagccct ggaaaaaggc
     4381 gttcagcacc cagagcacgg caaacgccca tagcgacgtc gagaagccaa acagaatgtt
     4441 catgatgccc gtggcgataa gccctatccc cataaaataa cgggcatttg agcgatcgct
     4501 gacaatgccg gagacaaact tcgacacgcc gtaagtaatg taaaacaggg tcgctaacag
     4561 gccgatatcg ctacggctga gcacgccgtt agcaaggatt tctggtaccg cggcgttaaa
     4621 acttttccgc gtgaagtaaa acagcgcgta acccagccag atggtcagca gaatatgccg
     4681 acgccagtag cgatagcggg catcaatttc atatttatca gtcattaatg gcgcatcggc
     4741 aggcgctttc agaaacggca acatcatcaa accttagaca tagcgttgag gtagagaaac
     4801 gctgacacgc gtgccgtgca gacaggaaat gtgtaatgtg ccacccagcg ccgttacgcg
     4861 ctcgcgcatt ccggtgaggc caaaaccttg ttgcccggaa cccggcggca aaccgctgcc
     4921 atcgtcttca ataaccagca tcaaccgttc atcctgctgc cagccttgca gggtgaccgc
     4981 gctggcatca gcatgtttca caatgttgtt cagcccttcc tggcagacac gaaacagcgt
     5041 cacgcgctgg ttttcgctta acgctgattc atcgattcgc cattcgagat ggctgacaat
     5101 accgcgccct tccagctcca tttcccgcat cagtgagcgg atggcctgct ccagggtgag
     5161 atcatccaac tggcgcggac gtaaccgacc caacaaacgg cgcaccgcgt cgtaaacgcc
     5221 cagcgatagt tgttcgatga gctgcccgct ctgcttcacg ctggcgttat ctgccgccag
     5281 ccgctgaaca atgcccgcct gagtacgaat ggctgtgatg gtctgaccga tatcatcatg
     5341 cagctcacgc gccacatcac ggcgcacgct ctcttcggtt tccagcaacc gttcagccag
     5401 atgctgattg cgcgccagtt ccttttgcag cgactggtta agttcacgca accgctggat
     5461 gccagcgcca agcaacaacc ctgtcagact ttgcaccagc agcgagagca ataaatccac
     5521 cggatgatcg cgccaggttt gactggcgat cagcgcgatg gcgttcatca acgtcgcaat
     5581 cagcgcccct tgccaaccat agtgccaggc cagcgcgata atcggcagcg ccagacaaaa
     5641 tggcgtaaag cgcgacagtt cgtccggcaa tcccaactgg agccagagac tgataacaaa
     5701 cagcagcaag taccagacca gatgtcgccc gcgccagttg atcggctgag aaaccagtga
     5761 cgggccgagc ggcagccagg tgttattggc gagatagtgc cagaacacca gacatatcgg
     5821 ggccagcgtc aggccgccag ttaaagtcag caacagcgca ttccacgact ctttgccgtg
     5881 ccaaagccag ggcagcgact gcaacaacgc cgccgccgtt aacgccgcac cctgtaacag
     5941 caaggtgcgc cagtcacgct gatggcgata gcgcgagatc agcgccacgg gcagtaacgt
     6001 cagtaaacta ccgatcatca ataacggaaa atgggttaaa ccgaccgcct gcgttagcca
     6061 gtaaatcagc agccactccg cgcccagcag caccggccag tatccgcgcg ggcattgcag
     6121 cattagcccc agacgcagac caaacggaaa taacagcacc gccatatcag ggcgctcaac
     6181 caggtgcagg ctgatactcc acaggcaaaa ccatgcggca gagaagataa aaaagcaggc
     6241 aataacggta attaagcggg agaacaacgt cttcatcacc agccatcaaa catgcggcgc
     6301 gccagctcta cgtcgttact gacacccagt ttttccatca ggttggcgcg atggacgtgc
     6361 accgttttcg gcgacaagcc cagttcggca gcaatctctt tcaccgccat tccttgcgcc
     6421 agtttttccg ccacctgccg ttcgcgtttg gttagcgggt cctgacgacc ggatgccagt
     6481 ttaatggcaa tatccggcgt cagataacag ccgcccgtgg caaccgtatg caccgccgca
     6541 atcagttcgt cagggctaca gcgtttggag agaaagccgc gtgcccccgc gttaagcgcc
     6601 tgctcaacca gcgccggact gtcatgaaca gagagcatta ttgtcgccat acctttcggc
     6661 agctggctta gcagttccag accggagata tcgggcatgg agatatcgca aatacacacc
     6721 tgcacaccgc gccccggcag ctgcgcaaag ccggagcgga cgatgaggtg atcgtctata
     6781 agggcaacgg tgatcatggt cttgtcctgg cgggtaaaaa aaaacgcgct taccttaacg
     6841 ataagcgcga tgttgttcaa gccttgagcg gttactgaaa aaacaccgca atcttgttaa
     6901 acatagtcgg atcggactga ttacgctgca ctttcaccac atcttccagc ttatcgattt
     6961 ggcttatcat ctgctccaga cgctggtcgt cattgaccag tagccagata tggcttttgt
     7021 cgctgtcctg aatcggcaga caaagaatgc cttcaacgtt aaaagcgcgg cgggcaaaaa
     7081 ggccacaaac gtgggtcatt acgcccggat ggttgcgaac ggtgagctcc agaatcacgt
     7141 tgtcatgagt tgtgttttgc atggcttatt cccccaccat ttcagtattc gccgcacctg
     7201 gcggcaccat cgggtaaact ttttcttcgg catcaatgcg cacatggatc agcgccgggc
     7261 cagggcgatt gatgatttcc tgcaatgcag cctgcggatc ggcttcgtta ttcaaatcac
     7321 aggtttcgag gccgaatccg gcggcaatct gcataaagtt gatttttccc ggataggtgg
     7381 cggcaaaaac gccttgctcg tagaacagac tctgttgctg atgcaccagc cccagcgctt
     7441 cgttgttcat cagaatgatt ttgacatcca gctgattttc actggcggtc gccatctcct
     7501 gaatattcat catcaggctg ccgtcgccgg agaaacacaa cactttgcga tccgggttcg
     7561 ccagcgccgc gccaatcgcc gcaggcaggc caaaacccat cgtgcccagc ccaccggagg
     7621 tcagccactg gcgtgggcga ttgagcggat aagcttgcgc ggtccacatc tgatgctgac
     7681 ccacatcggt ggtgataatc gcattgtcat cgacacaggc ggcaacggcg ttgatcaggc
     7741 cgtaatggct taatggatcg cacgctttcg ggattggaca cgggaattcg cgctgcaaat
     7801 ccgctaccaa ctggtgccac tctgcacgcg gttgcgcttc cacctgcggg atcaattgcg
     7861 ccagcacgtc atcaacatcc gcctgaatcg ccacatgcgg ctgcttgatt ttacccagct
     7921 ctgcgcgatc gatatcgaca tgaataattt tggcattcgg acagaactgc tcggttttgc
     7981 caatcgcccg gtcatcaaaa cgcgcaccga gcactatcaa caaatccgcc tcctgcaaaa
     8041 tatagttggt gctgcgcacg ccgtgcatcc ccagcatacc cagcgacaac ggatgcgctt
     8101 ttggcaacat gcccagcgcc attaaagtca tggtggtagg cagttgcgct ttctccgcca
     8161 gttcacgcac ccgtgcgggc gcattgatca caccgccgcc cagataaagc accgggcgtt
     8221 tggcagcgtt aatcatcgcc gctgcgtcac gaatgctttc ttcgctaaag gcgggggcgg
     8281 cggctttttc tgccatagcg ggctgtgttt caatctcaaa aactgccgtt tgcacatcct
     8341 taggaatgtc tatccacacc gggcctgggc ggcctgattg cgcaatgcgg aaggcatcgc
     8401 tcatgacctg cgggagttct tcgatatgtc tgaccagata gttgtgtttg gtgatgggga
     8461 tagagatgcc gtaggtgtcc acttcctgga agacgtcggt gccgatcatc gaggcgggaa
     8521 cctgaccggt gatgcaaatc agcgggatgg agtccagccg cgcatcggca atggcggtca
     8581 ccaggttagt cgcacccggt ccgctacagg ccatacagac cgccggttta ccgtcggtgc
     8641 gcgccatccc ctgagcgata aatcccgcgc cctgttcatg acgggccaga atatggcgga
     8701 tttgcgtgct ttggcttaag gcatcgtaaa caggcaggat agaaccgccc ggaatgcccg
     8761 tcacaatctt aatgccctgc tgttccagga aatgaacgat aaattctgcg ccggtaaagc
     8821 gcttacgcgt cgatgttgtg cccgaacttg ccatgctcca gtccttttct tctgggccga
     8881 ctttccggga ggtgcttaaa cgaaaaaccc cgcccggttt gcgccggcgg ggttttggaa
     8941 tcgtgtgttg ttccagtccc tacggcgcat tgccgacgac caccaccaca cgcacgacga
     9001 ccactgcggc ggatagcgca gttggtagta gttttgcgtt gaacatggaa gtagtcattg
     9061 gggaccttgt tggttttgtg tttaacaata tttatacaag cacagcttta caggggagac
     9121 aatggaaaat ttttcagcaa gggaaaattg aggggctgat cacgttttgt actgaattgc
     9181 aggtaacaaa aaaccccgcc ggagcgaggt ttcatcagcc gcctgcggct ggtaaccgca
     9241 aagcacactg tattatgtca acactgaaag tatacgtgtt ccgcgcagaa cgcgcaattt
     9301 cggcacgaat tttgacgtat ttagggcata gttgagtatc gatcacagtt tgcgttttgt
     9361 ccaaatatta ctgtttattt atacagtaaa cttctataat atcactgtac gcaatgtgtt
     9421 atgcgggggc cgcatcgtta cccggcgcac taagtcctgg ctgaaacggg tggtgccgtc
     9481 agcgccttaa ccccgcgtga gcacactgtg ttatgtcaac aagcacaacg tttctcctta
     9541 agataccgcg tgcacaacag ctggcaacag gcagcggaaa ggtacgtcag ctggcagtgc
     9601 tcctgaacca caggagacgc gtatgagcct ggtggatatc gccattctta tcctcaaact
     9661 cattgttgca gcactgcaac tgcttgatgc tgttctgaaa tacctgaagt aattcagatt
     9721 caagtcgcac caaaggggag cgggtaaccg ctcccctttt atatttagcg tgcgggttgg
     9781 agttgggggc ttcttctacc agcagaatgt ttgcacctgc gctgcctgtg cccctcaccc
     9841 tagccctctc cccaaagggg cgaggggacc gtcccgtgca atgtttagct cattttctga
     9901 aagggaacgg tggtttcccc ctcgcccctt tggggagagg gttacagttc gcacgtaatc
     9961 cctcccggca acatttattg ccgcttttgt ttacatattc tgccgctaaa caattcccca
    10021 ttcctggcgt atatctggct aacattcatc aatgtgatag attcctctcc cgcatttatg
    10081 ggaatgcgta gtgacttatt ctaattattt ttataaaagc atccgtgata atgaaaaggc
    10141 aaagaaacgt caatttgtta ttgatgttgg tattactcgt ggccgtcggt cagatggcgc
    10201 aaaccattta tattccagct attgccgata tggcgcgcga tctcaacgtc cgtgaagggg
    10261 cggtgcagag cgtaatgggc gcttatctgc tgacttacgg tgtctcacag ctgttttatg
    10321 gcccgatttc cgaccgcgtg ggccgccgac cggtgatcct cgtcggaatg tccattttta
    10381 tgctggcaac gctggtcgcg gtcacgacct ccagtttgac ggtgttgatt gccgccagcg
    10441 cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc gcgtactttg ccgcgagatt
    10501 tatatgaacg gacacagttg cgccatgcta acagcctgtt aaacatgggg attctcgtca
    10561 gtccgttgct cgcaccgcta atcggcggtc tgctggatac gatgtggaac tggcgcgcct
    10621 gttatctctt tttgttggtt ctttgtgctg gtgtgacctt cagtatggcc cgctggatgc
    10681 cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct taccagttat aaaacgcttt
    10741 tcggtaacag cggttttaac tgttatttgc tgatgctgat tggcggtctg gccgggattg
    10801 ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt gttagggctg agcagtatga
    10861 cggtcagtat tttgtttatt ctgccgattc cggcagcgtt ttttggcgca tggtttgccg
    10921 gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc ggttatctgc tgcctgctgg
    10981 ctggcttgct gatgtggatc cccgactggt ttggcgtgat gaatgtctgg acgctgctcg
    11041 ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt tccgctggcg accagcggcg
    11101 cgatggagcc gttccccttc ctggcgggca cggctggcgc gctggtcggc ggtctgcaaa
    11161 acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt gccgcaaacc ggtcagggca
    11221 gcctggggtt gttgatgacc ttaatgggat tgttgatcgt gctgtgctgg ctgccgctgg
    11281 cgacgcggat gtcgcatcag gggcagcccg tttaagcgca cgtcaccgca gcatcgtcat
    11341 cagctccatg ggagaacgat gctgctttat cagatcacgc atcacccgca tatgcggtgc
    11401 ggagtaagaa taaaacgcct gatagcccgc acaaagcacg ctgtctggcg tctcctggcg
    11461 agcgtgcggg cacaggcgat gccagtcgca cgcctggcag tcacggcgcg gttgcgggtt
    11521 ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc tcaatcgaga tacgactgat
    11581 atctgcccgc acccaacact cgaacagggc aattaaaaat ttcccccaca gctctgctgt
    11641 caccgaagcg gccgtcagat cgccccggtc atcaaattca agatagggaa tgagctgtac
    11701 ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg gcaggttgca gagcatcctt
    11761 gtactctaca accagtttat gcctgtcctc ttcagcatca atgacctgac ttcctgtcat
    11821 gcgactctct caataaatac gataagttga tgtatatgcg ttacagcaaa cagtattgcg
    11881 agagataaca ccgcgaggga gatcaaaaat ttgtcacgga ctacgtgaaa ttgggaaaaa
    11941 tcgctattcg tgacatccat taaattgcga ttacgggtgt agtgccagag gatcagcgcc
    12001 acgatggcga gaatgccaat cgaaatccag aataacatgc ccgcctggtg ccagttgtgt
    12061 ttgatagcca acgccatcaa cgcgccgtag cccagcatgg tacgaaacca cgccagtgat
    12121 gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg ctttgcggct atccggcata
    12181 caacaccagt cccataacga tcaccgccac gaccatcaat attaagctaa tgattaataa
    12241 gctgttggtg tagggcaggt cctctttcag acgcatcgct ttttcattgc gtaaccagcg
    12301 caggtagcca tacatcgcta acccgccgga aaacagacac aacaacaacg ccagcagttc
    12361 acgaatgacg ggcgtggcga aatctggcgc aagttgatca agcccaaccc ccgccgccag
    12421 aaatcctagt gcggtacgga tccacgccag aaaagtacgc tcattagcca gcgagaagcg
    12481 gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt gctcctttgc aatttcacta
    12541 acgctctacc tttagcggtt tttttttaag aatttcgtcc gcccgtttcc tgatatgctt
    12601 tcgccatccg ctacaaggag gtcagatggg tatcatagct caaaataaaa tcagttcatt
    12661 gggcatgctg ttcggtgcca tcgctttaat gatggggatt atccactttt cattcggccc
    12721 tttttccgca ccaccgccaa cgtttgaaag catcgtggcg gacaaaacgg ctgaaattaa
    12781 aagaggtctt ctcgccggaa taaaaggcga aaaaattacc accgtggaga aaaaagagga
    12841 cgtagatgtc gataaaatcc tcgatcaaag cggtatcgca ctggccattg ctgcgctact
    12901 ttgcgcattt attggcggta tgcgtaagga aaatcgctgg ggcattcgcg gcgcgctggt
    12961 ttttggcggc gggacgctgg ctttccatac cttgcttttt ggtatcggca tcgtgtgcag
    13021 cattttgctg atattcctga ttttttcctt cctgaccggc ggttcactgg tttaagcagc
    13081 ctgaataatg cccgccgggt gttcatcagc gggcattatc gtcagaactt ctgttttttc
    13141 tcttccactt tcactccttg cgtcggtagg ccggtgtcat agtcacgtac caccggcgaa
    13201 tagccatctt gtgggcgtgg acgaaatgcc cccatccagc gcggcagtgc atctttacgc
    13261 cacggacgca ggctccattg gtaactgcgg aacggatcgc gaattttgtc catgtaatcc
    13321 aataaggcgt catgcatttt gctgcgaacg tctgcaaaac ggatatcatc gatcaggtta
    13381 tgcatttcat ttgggtcatt acggcgatcg taaagttcat cactggtgaa gaggttgagt
    13441 accagtttaa agtcatccgt cacccagcaa cgcaccggaa taaaaccgcc aaagctgtca
    13501 tgctcaatct cgtagcggtt aaattccacc attacaccgc gtggctcttt cacggcaagg
    13561 atattttccc ccggcagaat ctctggtttt tcaatatctg ccagcgccat cattgtcggc
    13621 agtaaatcga tatgactgac tggcgtatcg acctgtcgcc gctccccttg cggcgaacgg
    13681 atgatcagcg gaatacgggt gatgtcgtca tacatcgccg ctcctttact gatcagctta
    13741 tgtgcgccca tcatttcgcc gtgatcggaa gtataaatga cccacgtatt ttcacgttgc
    13801 tctggcgtta aggcattgat gacccgtccg atttggtcat caacaaagtc attacaggca
    13861 aaatagagcg gatggtgata aagcccgtca tcaccgactg gcgatggcat cgcctgcgcc
    13921 cataagcggt gatgttccgg tttgttcgcc aggccatcct gagctttctc gcccagatcg
    13981 tagtaaaaat cagcgtattt ctctaaatac tccaccggac aggtgaacgg gtgatgcggc
    14041 tcatcatacg aaaccaccat caggaagggt tcctcggcgc gcgcgggctg ttgcagaaaa
    14101 tctactgccc gattgctgat gcggtgcgcc caggtgaagg tttcgtcgat atggttcgcc
    14161 tgtaaatcct caacgctgtt taggccattg cgccacaggc tgatctcttt ttccgtcagt
    14221 tcgctaaggt agttcgcccc atcgaaccag taatcagcgt cccactccgg cggacactcg
    14281 ccagtgccga aatagtcatg accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag
    14341 ccggcatcct taaagtagcg ccccatagtg gagatgtttt tgcctggcgc gacgttgttg
    14401 gtccacgggc cggactggtt agcgtagata ccggtaaata gtccggcgcg tgcaggcgta
    14461 caaaccggtg aacaggtgta ggcggaatta aagcgaatac cttccgccgc cagactatca
    14521 atattttgcg tattcagcgg gttaccgcta tagcaaccga ccatattggt ggcctgggta
    14581 tcggtcatga tgaacagaaa attggggcgt ttcataggct atccttcgcg tcgggagtaa
    14641 aggttacggc tgggtcgtga cgatgccgcc agctgtcaaa aatcaggtag acaatcacta
    14701 cggcggcaat gaaataactg atcatcgcta accagcgcgt accgtagccg ccgaattcag
    14761 ccagcccggc atagacgcca atcatcgcga acaggatgcc aattgacgcg atcttgacgt
    14821 ttctccacgg tttcatgtcc accgcaaacg catctttgaa ggtgaacggc gtggcgcgcg
    14881 gtttgataaa accgatcacc agcatcacga ccacgttgat gcagaacgta caggccagca
    14941 catagaggaa atggaagtcg aacttcacca gatagttgat ggtgatgtag ctgattatgc
    15001 caatccccat cgccactttt gccgccagcg ccgggatgcg cgggaagaaa aagcccataa
    15061 tgatgatggt aaccagcggc acgttgtaaa tgccgttgag ctgtttcatc cagctataca
    15121 gcccctgcgg cgcgttggcg atccacggcg cgaccagcac cgaaacgata gcgataaaga
    15181 aaccaaattt gcgcccgacg gtgaccagct gctgcggctc ggcattctgg ttaatgatgc
    15241 gacggtaaat acccatactg aataacgtac tggcgctatt cagaaagccg ttgaaggtac
    15301 tgatcaccgc accaaataac accgcgccga agaaacccac cattggcact ggcagaacgt
    15361 tattgaccag cgtcgggtag gccatgtcgg ctttcggtaa atcctgatac agatgaaacg
    15421 caatcaaccc tggcagcacc agtaccagcg ggtcgagcat tttcagcacc gccgttaaca
    15481 gcgccccctt ttgcccttcc gccaggcttt ttgacgccag cgtgcgttgc acgatgccct
    15541 gatttgtaca ccagtaaaag gtgttcacca gaatcaaacc ggtaaatgcc gcgccaatcg
    15601 gcaagggatc ggtcgggcca ccgattgagt ttaatttctc ggcgtgaacg gtggtgagtt
    15661 gctcaatgcc ctgcataaag ctgcccttgc ccatcgcgat caggccaaat accggcacca
    15721 tcaacccgcc gataaccagc ccaataccgt tgatggagtc cgccactgcc attgcgcgca
    15781 gtccgccgat caccgcatac aaaattcccg ccagaccaag caaaattacc agtagccaga
    15841 tagccgcacc gtgagaaatc tgtagcgatt ccccgacgtg aaacaggctg ttgagcgcca
    15901 acgcgccgga gtagagaaca atcggcagaa agcagacgcc ggtggcaatg aggaagcaga
    15961 agtcgatgat aatacgcgtc gttttatcat aacgttcctc cagaaaatcg gggatggtgg
    16021 caatgccgcg cttcagatag cgcggtaaaa agattagcgc gaggaagatc agcgtcaccg
    16081 ctgaagtcac ttcccagccc atcaccgaca tgccgctttt gtaggcctgc ccggaaaggc
    16141 cgaccagttg ttccgtggaa aggttggtta gcattaacga agcggcaata accggcgctt
    16201 ttagtgaacg tccggcaaga aaatagcctt gttgcgatcc ggtatctgtt ttgcggacct
    16261 tccaccaggt gatcaccgcc accagcagcg taaaaccgac aaaactcaag atttgtaacg
    16321 aattcatctc atagccctta tttatgttta tgtcccggac gcatgaatat agggagaggt
    16381 aaaatgcgtc gggtcaggca gtcagattct taaaatacaa acgtcgtatc cctgaacgga
    16441 ttgttttctg ttaagttcag gttgtgagca taattcgccg gagggatatt aaaatgaatg
    16501 gaaaattgca aagttcagat gtaaaaaacg aaactccgta caatattccc ttattgatta
    16561 acgaaaatgt gatctccagc ggaatttctc tgatctcgct ctggcatacc tacgccgacg
    16621 agcattaccg ggtgatctgg ccgcgggaca agaagaaacc gcttatcgcc aactcatggg
    16681 ttgcggtgta taccgtacaa ggatgtggga aaattctttt aaagaatggc gaacaaataa
    16741 cactgcatgg caactgtatt atattcttaa agccaatgga tattcactct tatcactgtg
    16801 aaggtttagt ctgggaacag tactggatgg aatttacccc caccagtatg atggatattc
    16861 ccgttggtca gcaaagcgtt atttataatg gcgaaattta taatcaggaa ctcaccgaag
    16921 ttgctgagtt aataacttca ccagaagcaa taaaaaataa tctggcagtc gcttttctga
    16981 cgaaaattat ttatcagtgg atttgtctta tgtacgcaga tggtaaaaaa gatccacaac
    17041 ggcggcaaat tgaaaaatta attgccactt tacatgccag tctgcaacaa cgctggagcg
    17101 tagctgatat ggctgccacg atcccctgta gcgaagcctg gttgcgtcgt ctgtttttac
    17161 gctataccgg caagacgccg aaagaatatt acctcgatgc gcgtctggat ctggcgctat
    17221 cgctattaaa gcaacaagga aactcggttg gcgaagtcgc tgatacgctc aacttcttcg
    17281 actcctttca tttcagcaaa gcctttaaac ataaatttgg ttatgcgccg tcagccgtgc
    17341 taaagaatac ggaccagcac ccaacggatg ccagtccaca caattaacgt aactctggcc
    17401 agtacgcttt gttggcctcc agcaactcat ccagaatagc tttggcgacc gaggcgctcg
    17461 gtaccgtttt cgacaacgta atcgcctgcc acaggtgctg atatgaacgc tgttcccagg
    17521 cgtccacgac cagtttttcc accgccactt gctgactcat taaccctttc tgaaactgcg
    17581 ggatatcgcc gaccaccagt ggttcaggcc gtagtagcgc acgcgcatct gtttgcgatc
    17641 ttgcagccca acaatttgcg ccatccggct ttcaataccg attggcatgt cacagatgtt
    17701 gaggattttc gcattcgggc gcagacgacg cgtggcttct gcgacaattg ctgccgggtt
    17761 ggagtagttg agcatccagg cgtttggtga atatttttcc atataatcca ccagttccag
    17821 gacgccgcca atggaacgca tgccgtacgc tattccgccg ggtccgcaag tttcctgacc
    17881 aacaacaccg tggcgcagtg ggattttttc atccagttcg cgcatcgggt acttgccgac
    17941 gcggatgtgc gccatgacaa aatcaacgtc gctgaacgct acttcaggat cggtggtgta
    18001 actaaacgca atgtccggcg ctttttcttt aaggatgact ttacaggctt cggcaatcac
    18061 ttcctgccgc gcgccatcgt tatcataaaa tttcagtgca cgaagcggga aacggtcctg
    18121 attcgccagg agcatcaaca cgatgcctgg cgtaaaggtg cttccaccgc ctgcgacaac
    18181 cactgagaat ttggtcatta tactgcctcc gtaatggcaa cattttctgc tgattgatga
    18241 gaattaatta agctatcgag ctgttcacgc aattgggata catgcagacc aatgatcacc
    18301 tgaatggcat cgccactacg gaagacgccg tgcgctccca gctttttaaa gacttcgtca
    18361 tccagcgttt gtgacatgtc atgcagtgca atgcgtagac gcgtcgcgca attgttaatg
    18421 ctggagatat tgccgacccc gcccagggct tgcagaatac cggcagcctg atccagctct
    18481 ttttttggct ctgccgcggt ggtttggcct cgcgaggctt tgtattcggc ttttgagtag
    18541 agtttcactt ccgcatcttc acgtcccggc gtgcacatgt tgaactgcag aatcagggtg
    18601 cggaaaacca cgaagtacag cagggtaaag cacaacccaa tggcgatttg ggtcagcatc
    18661 atatccgcgt ggttgctgaa catcgggatc cagttttgcg gtaaaacctg gtcaatcaga
    18721 cctccgccca tgttgcccac cacaccaaag agatacatca cggtcgacat tgaggccgcc
    18781 agcacggcgt gtaccgcaaa cagcaacggt gaaatgaaca ggaaggtaaa ttccagcggt
    18841 tcggtaattc ccaccagcat ggcggttaag gttgcgggaa tcagcaagcc cgccactttt
    18901 acccgatttt ccggtgcggc agtgaagtac atcgctaaag aaatgcccac ggcaccaaag
    18961 atttttgagt taccgtgcag ggcaaaaccg ccttccggga acaacgattt cagcggctcg
    19021 gcgctcagac tgaactcttg cagatgctgc gcccagtaca tctgaatgcc gccttcaaca
    19081 gctgccggac caaagataaa cggtccgtag atgaagtggt gtaaaccggt tgggatcaga
    19141 atacgttcga ggaaggtgta aacccacacc ccaagcgcac ccgccgaacg caggaacgct
    19201 tgcagagatt caatccccat ttgtactttt ggccagccga gcaacgtcag ccaggcacag
    19261 gggatcatca ccaggaaggc gataatcacc acataagacg tcccctggaa aatgccgaga
    19321 aaaaccggca gttttttatc gaacagacgg ttatgcagcg ccgtcacaat gccggaaatg
    19381 ataattgcgc cgataatgct ggtatcgagg gttttaatcc cggccatcat tgtcagaccg
    19441 ctacctgcca ccgcgtcctg agtgaaatcg acgccgaagt agcttcccca ggtcattccc
    19501 atcgcgttga tgaaatagtt ccaggtcagg aaactcacca tcaccgccag acaagcacgc
    19561 ccctgcgctt gcttagcaag gccaatgggt aaaccgacag caaaaatcag cggcatatta
    19621 cggaacaccg tccaaccgcc ctcttcaata atgtgtacga tttgcgcgaa taaactgttc
    19681 ggatcggtca gtgattcccc gacaaacatc gggttttgca gcaagatggc aagacccacc
    19741 acaatcccgg cgaagggaaa cagcagcact ggcgtgaaca tcgcgccgcc aaagcgttga
    19801 atttgactga gcatttccac atcctctttt ctcaattctg taagtaggaa gcctgattgg
    19861 ttatgcggca ggcctggagc gaggatagga agatgcgatc ggcagaacat gacgacccaa
    19921 tgggattcgt gatcgctttc atgcttttcg ttgggcggta gagatctacg ttttcgcgaa
    19981 aaagtagaca tctgtgagca caggtacaca gcatcgaaca catctttaaa aaaaagatgt
    20041 tttttcaatc gattaagcag aacttgtggg cgcattaccc gtgcttgcag gcaaaaaaga
    20101 gatctagaga tgatctacaa aagcattgcg gagcggttaa gaattcgact taattccgca
    20161 gatttcacgc taaatagcct tcttcccggt gaaaaaaagt tggcggaaga atttgcggta
    20221 tcgcggatga ccatccgtaa agccattgac ctgctggtag cgtggggatt ggtggtccgc
    20281 cgccacggca gcggcactta cctggtgcgc aaagatgtac tgcatcaaac cgccagcctg
    20341 accgggctgg tggaggtgtt aaaacggcag ggaaaaacgg tcaccagcca ggtgctgatt
    20401 tttgaaatca tgcctgcgcc tccggccatt gccagccagt tacggattca aatcaacgag
    20461 cagatctact tctcccgtcg cgttcgtttt gtggaaggga aaccgctgat gctggaagac
    20521 agctatatgc cggtaaaact gttccgtaat ctttcgctgc aacatctgga agggtcgaag
    20581 tttgaatata ttgaacaaga gtgcgggatt ttgattggcg gtaattatga aagcctgacg
    20641 ccagtgctcg ccgatagact gctggcgcgg caaatgaagg tagcggaaca cacgccactg
    20701 ctgcggatca cctcgttgtc atatagcgag agcggggagt ttttgaatta ttcagtgatg
    20761 ttcagaaatg ccagcgaata ccaggtggag taccatttac ggcgactcca cccggaaaag
    20821 agttaaccga tactccagaa gagcaccgcc agtaattggg gggtgataat gcgcaggaac
    20881 atcactaacg gatagacagt ggcgtaagag agcgccgccg caccgctggt tggatgaaga
    20941 ttattagcaa acgccagcgc cggaggatcg gtcatggaac ctgccagcat cccgcacatg
    21001 gtcaggtaat tcattttggc taacatccgc gccagaatgc caacagtaat cagcggaacg
    21061 gcggtgatca gggcaccata accaatccag cttagccctt cgccattgac cagggtattc
    21121 acaaaatctc caccagattt cagaccaacg accgagagga acagcacgat ccccagctcc
    21181 cgcagcgcga ggttggcact tggcggcata aaccagtaca gcttgccgat actgccgata
    21241 cgcccgagga tcaacgccat aatcagcgga ccgcccgcca gccccagttt caacgcggcc
    21301 gggaacccag gcacaaagac gggaatagaa cctaacaata cgccaagccc gatgccaata
    21361 aacactggca gcatctgaac ctgttgcagt ttttgctgcg cattccccag cacattggca
    21421 acggcatcaa ttgcggacgg acgccccacc agattgagga tatcgccgaa ctgcaggctg
    21481 atatcgccac tggcgaccag ttcgaccccg gcacggttca ggcgcgagat aacaacgtca
    21541 tagcgttctt taaagtgcag gtcgcgaata cgttttccga gcacgttttc attggtgacc
    21601 accacacgct cgacgcgcaa atcagtgcct ttcgtggaca gcgaagtatc gacctcctga
    21661 ccaatcacca gttgcgcatt atgtaaatcc gctggctgcc ccaccagatg cagcaaatcg
    21721 cccagttgga taatggtatc tggcgaagga acttttaggg tttcttcgcg tttcagacgc
    21781 gagcagataa ttttgtcgcc gttgagaatc ggtacatctt taatggctaa atcatgcagg
    21841 ttagggttct caacgcgaat attgatagtc ttgatcagcg cgccgccatt ggtgcgtgaa
    21901 gactcgtgct gctgagcttc tgtctcgaca ttgacgcgga aaataacccg caacatccac
    21961 atggtgaaca aaatcccgca aatgccgaat ggatacgcca tcgcgtaact catccccatc
    22021 tgatcgacca tttccattgg tgtacccaag tcgcgcaaaa tctgctgccc tgcccccagc
    22081 gctggcgtat tggtaaccgc accggagaaa atccccagca ctaccggcag tggaatatca
    22141 aacagtttat gcaggatggc ggtaaccaga ccaccgatga tgacgatcag aacagcaaac
    22201 aggttgaggc gtaatccgga gacgcgcaat gaggcaaaga agcccggccc tacctgaatc
    22261 ccgatagtat aaacgaacag gatcaggcca aattcctgaa taacatgcag catatcgcta
    22321 cttaatgtca tccccgcctg agaaacaaaa tggccgacga tgatcccacc aaacagcacg
    22381 ccgccaatac ctaatcctat gccgcgaaat ttgacattgc cgataaacaa accgacgact
    22441 gccaccaaag ccagaatact gaccgttaat gctatatcac tcatcgtcca tttctcttga
    22501 gataacattt ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag
    22561 cgcacattat cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat
    22621 tgttgagtaa gtaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag
    22681 ttagctattt aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg
    22741 ctcattacga attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc
    22801 catattttca gccagcgtaa agctcaggct aaatggctga ttcataagcc cttgatgcag
    22861 ccattttttc tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtaccttc
    22921 cagttgaatc tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg
    22981 gttatcgtcg cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg
    23041 cagtgcgttg gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa
    23101 gttacgcata gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc
    23161 gcatatgctc cctaacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa
    23221 ttagttgatt tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat
    23281 atacagcaaa ccatttacca ggttagcgcc acgaacatga atgttctcag ctaactggaa
    23341 tttgcgttca aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc
    23401 gtcggcgtga gcacctttca ccactagcag attatcctgg gcggtaattt ccagttcgct
    23461 ctcagcaaaa ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac
    23521 gttatacgga gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa
    23581 acggtcaaat ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa
    23641 tagctcctga aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga
    23701 acacccatca ggcgcgtcct catcggctac gatgtaaaaa tgggtctgga aatgaacttt
    23761 tcaagcctta tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg
    23821 cagcacgaaa cgttaaagtt cgatagccgc cacagtgcga cgtaatgatg atgttatttt
    23881 cagcaaggga tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta
    23941 tgataagaaa tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat
    24001 gctccagcgt gatgtcccat accggaggga aagaaggaac gtatcccggc acccgcgcca
    24061 gcgcaaccat gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg
    24121 atatgccgtt taccgctgtg atggacacgc ttttactgcc gtgggatgtg ttccgcaaag
    24181 acagctcggt gcgctcacgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg
    24241 ttattccacc ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag
    24301 ctggccacct tccatccacg ccagccattg accatccggc gagaagacct cggcgtccgc
    24361 agaaggtgga ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc
    24421 acaagcaatt cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa
    24481 tgcagactga atatccgttt tgttatgggt taactggcgc ggctcgccgc cctgtggcga
    24541 gataagccac agttgcacaa tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc
    24601 ctgcggatta cagcgcaccc agtggcgcgg gacgttgact aaccccggat aagcccgatg
    24661 gtgggtaaag gttaaacgtc gctgcacgac gccgcgcggt ggcgcgggca gggtggtttc
    24721 cgttccactt aacggcgcat cacctgccgc tttccagcca gcttcatctt gcggtaactc
    24781 aacgataaac agctccggca ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc
    24841 cagcgcgtga tttcctaccc atccttcttc ataagcacga ttgatttcat cgctgccagg
    24901 ctgcggcgtg ggcgtggttt tactcaccag cacgcaccag tggctaccgc tgtattcacg
    24961 cggatgctgc ttttgtacgt tgaccgggcc aaacggcgca gcaacaccga cgtttcgcaa
    25021 atccagcgcc ggatcgagtt catgcattac atggtcgtta taggtaaagc tcaccctttc
    25081 accgttcggg ctaaagacat gcacatggct gccgccgcgc agcgctcctg gcatatacgg
    25141 cgcggtaata tccattgcat cgagattgct caccttgccg ccttcagcaa tgactccgcg
    25201 tcgatgatgg aaatcgtaat gccatgtttc atcaggattt tccgggccat ggatgaaaac
    25261 atatttctct gacttcggat gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg
    25321 atagataacc tcgacctcgc cggtatggat attcacacgc tcaatggtct cgccggtaaa
    25381 cgacgcccca gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt
    25441 ggtattggtg agcaggtgat tacggggagc aaaggttatc tgtttcatgg cgcggtcctg
    25501 atacaaaacg ggtcccgcca tcatacttca caaggacttc actttcagac aatagtcttt
    25561 tgccatcgtg atgaatgtga gtgaaaagat ggaacatttc gacgtggcga ttattggcct
    25621 cggcccggca ggatcggcgt tggcgcgaaa attagcaggc aaaatgcagg tgatcgcgct
    25681 ggataaaaag caccagtgtg gtactgaagg tttcagcaaa ccttgcggcg gtctgctggc
    25741 accggacgcg cagcgatctt ttattcgcga tggactgacg cttcctgtcg atgtgatcgc
    25801 caacccgcag attttcagcg tcaaaactgt cgacgtcgcc gcatcgctca cgcgtaacta
    25861 ccagcgaagc tatatcaata ttaatcgcca tgctttcgac ttgtggatga aatcgctgat
    25921 ccccgccagc gttgagattt atcacgacag cctgtgtcga aaaatctggc gtgaggatga
    25981 taaatggcat gtcatttttc gtgcagacgg ctgggagcaa catattactg cccgctatct
    26041 ggtcggtgcc gatggcgcaa actcgatggt gcggcgacat ctctacccgg atcatcaaat
    26101 ccgtaaatat gtcgctatcc agcagtggtt cgcagagaaa catccggtgc cgttctactc
    26161 ctgcatcttt gataattcga taactaactg ttattcatgg agtatcagca aagacggtta
    26221 ttttatcttt ggcggtgctt atccaatgaa agacggtcag acgcgtttca cgacgctgaa
    26281 agagaaaatg agcgcctttc agttccagtt tggtaaggcg gtgaaaagcg aaaaatgcac
    26341 ggtgctgttt ccctcacgct ggcaggattt tgtctgcggt aaggacaacg cctttctgat
    26401 tggcgaagcg gcaggattta tcagcgccag ttcgctggag gggattagct atgcgctgga
    26461 tagcgcagac attctgcgtt cggtgttact gaagcagcca gagaagctca ataccgctta
    26521 ctggcgcgcc acccgcaaac tgcgtttaaa actcttcggc aagatagtaa aaagccgatg
    26581 cctgaccgca ccggctttaa gaaagcggat tatgcgcagt ggtatggcgc atattccaca
    26641 gttgaaagat tagccaacgc gcttcacatc gcccaccagc aggatgtaag agagcgcgcc
    26701 aatcaacgcg acggcggaga tataaaccag tgcaggtgcg aaaccgtaac cctgcgccag
    26761 gtaccccacc accagcggaa cggtgatgcc gcccagacca ccggcgaagt taaacacgcc
    26821 gccggttaaa ccaatcaggc gcatcggtgc cagagaagag accagcgacc aggtaatcga
    26881 agcaaaaccg ttgccgaaga atgccagcgc catcaggcac ataatcatca tcggatcgtt
    26941 ggtgtagtta gcgcccataa tgcaggtgga gatcagcaag ccgcagataa tcggcgtttt
    27001 acgcgcaaag cccagtgaaa agcccttacg taccagcaga tccgcgaccc agccagagag
    27061 caggacgccg acaaacgccg cgaggaatgg caccgtggtc ataaagcctg ctttcagcgc
    27121 cgtgattcct ttttcctggg ttaaatagtt cgggaaccag gttaagaaaa accacagtgt
    27181 agaagccacc gcaaattgcc caagataaac gccgatcagt ttacgatgga acaccagttt
    27241 ccagtctttg gctgttaacg gctgacgcgc ctctttcttc accggcgcat caccatccac
    27301 cagaccgccg ccatcacgaa tgtaatccag ttcagctttg ctgatacctt tggtcaggcg
    27361 cggcggctga taaaccttaa accaaatcag cgaccagata atgccgatac caccagtgac
    27421 aatgaacacc cagtgccagc tcaacatctc ctgaatccag atcagcagcg gagtcaaaaa
    27481 cgccagaccg acaaactgac cagacgtata aaaaccaacg gcggaagcgc gttcatgttc
    27541 cgggaaccag ctggtcacca tccggttatt ggtcgggaaa gcgggcgctt cgaaaatacc
    27601 ggttatcgca cgcagaccaa ttaatgacat taatcccgtg gcaaagccct ggaataaagt
    27661 cgccactgac caaccaaata tcgcaataaa ataagtcacg cgagaaccta cgcgatctaa
    27721 aaaccaaccg ccggggatct ggcatagcgt ataaagccag gcgaaggccg aaaatacata
    27781 gcccatttcc gctttcgtaa tgccgaactc ttcctgaata tgggcggaag ccacggccag
    27841 gttagcgcgg tcaacataac aaatgaccac cgtaataaag atcatcacca gcgtcagata
    27901 acgccgacgc cccggctttg ctgcattaac gggaatatcc atagcgagct ttctccagat
    27961 tttgggcata gcgaagccgc tcaccatgcc ctgtaattta cagagggtta tatttttgta
    28021 ttgctgtttt agtgcccgat gaggggctta cgtggcagga attaccactc tgctacgctg
    28081 ttatcttcat gacgccagag cggattacgc cagtccgggg catttttact gaactcaata
    28141 actttagctt cgtcgatttc cacacctaag cccggtttcg ttaacggttt aaagaagccg
    28201 ccaaccatac tgaagtcttc tttgtttttc acaaagtcga gtaactccgc gcctttgttg
    28261 taatgaatgc ccatactttg ttcctgaagt accgcgttat aggaaacaaa gtcgatatgc
    28321 aggcaagccg ccagtgcaat cggtccgagc ggacagtgcg gcgcaagggt cacatcatag
    28381 gcttctgcca ttccagcaat tttgtagcat tcggtaatac cgcctgcatg ggagagatcc
    28441 ggttgcagaa tcgaaatacc gcctgcctcc agcacgcgtt taaaatcgaa acgtgagaac
    28501 atgcgctcac ctgccgccag tggaatatgc gtttgtgccg ccaatttcgg gtagtattcg
    28561 gcttgttccg ccagcaccgg ctcttcaata aacagcgggc gatacggctc cagttcttta
    28621 atcagcactt tcgccattgg cgcgctgacg cgaccgtgga aatcaagacc aaactcaatc
    28681 tgattgccaa aagcttcacg aatttgtgcc acggtgttga ctgccgcatc taccgcgcgg
    28741 gagttatcaa ttagccccag ttcttcacaa ccgttcagtt tgaaggtatc gaagccgatt
    28801 tcgcgcaggg ttttaatgcc gtcgataaca tccgccggac gatcgccgcc gacccaactg
    28861 taggctttaa ttttgtcgcg aaccaggccg cccatcagtt gccagaccgg cgcattcagc
    28921 actttgcctt tgatatccca taacgcctgg tcgatcccgg caatggcgct catcaggatt
    28981 ggaccgccac gataaaatcc ggcgcgatac atcacttgcc ataagtcatt gatgcgcgaa
    29041 ggatcctgac caatcaaata gtcacccagc tcgtgaactg ccgcttccac cgtacgggcg
    29101 cggccttcaa tcacgggctc gccccaaccg accacgcctt catcggtttc aattttcagg
    29161 aacatccagc ggggaggtaa acgatacgtg gtaattttgg tgattttcat tgcactgcct
    29221 ctcgatacgc cttaacaaat gctgctgcct gctgcgcggt gcgttctacg gactgcccgg
    29281 cgcgatagag atcgctgcct aagcccgccc cagcacaacc tgcgtctatc cactgcgcca
    29341 ggttttctgg cgtcacgccg ccaacggcaa agactgcgat gtccgatggc aataccgctt
    29401 ttaacgcttt gatgtattgc ggaccaaaag ccgatgacgg aaatattttc agcgcctgcg
    29461 cgcccgcttc gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg cagacggtca
    29521 tgccgtagcc caccgcacgg cggatcactt cactatggat attgggcgta acgatgagct
    29581 gacaacccat cctggcgagc gcatcgacct gttcaggttt cagtaccgta cctgcgccaa
    29641 tcaacgcctt gtcgccatac gcatcaacga tggcgggaat gctttgctcc cattgtgggg
    29701 aattcagcgg gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca acatgcgcca
    29761 gcgcctcgtc gggcttaatg ccgcgcaaaa tggcgatcag cggaagttta gtttgccact
    29821 gcatgagcga tgctccttat accagcctga aatgccgtgt cgcccgccac cgccgtcacg
    29881 tcgcaaccca tcgcctgaaa ggcttgctgg tagcgcgcgg tcagcgatgt tccggcgaca
    29941 agggtgatgg cgtgttgatg ggtcacatag tcgcgcatac tggcaacctc tgcgccaatc
    30001 aacaaaccag agagaaattc gctgacctgt tcgcggggaa gtgttcccag cacatgcgag
    30061 gcgcgaactt caaaaagctg cggcaatatg gcgggcgcat taaggccgcg ctcaaggcca
    30121 gccgcgaagg catcggcaga gttttcctgc ggcggcaaac ctgcgccaat caatgagtga
    30181 tttaacagta aatgatgtaa ttcaccggtc atcacggtgc gaaaatcgtt gatttgctgg
    30241 ctatcggcct gcacccattt gcaatgcgta ccgggcataa cataaagaga ggaaggagcc
    30301 agagttcgcg cgccgatcaa ttgtgtttct tcgccgcgca tcacattgtg gttatcgtca
    30361 tgagagacgc ataatccggg aataatccag atattgtcgc caacagacgt taattgttcg
    30421 ccaatagacg aaaaacgggc aggaacagat aaatacggag caactttcca gccgacattg
    30481 ctgccgacca ttcctgccat taccactggc gtattctctt cacgccagtc ggtcgtgact
    30541 tctgctaaca ccgcagccgg agattttccg ttcaggcgcg tgacgcctgc ttctgattgc
    30601 ctgctctcca ggcagtggtc gccctgataa agccaggcgc gaagattggt cgatccccag
    30661 tcaattgcga tgtagcgagc tgtcatgtga tttcctttaa ccttcgtgtc gagctggcga
    30721 tcatggtaag cgccgcctgc tctgccgcat cgccgtcctg atgccgtatt gcatcgaaca
    30781 gcgccttatg ttcctggagc gtttgcggca tgttggcctc atcgcccatc caggttcgtt
    30841 caaaaaccgc ccgctgcaac gaactgatcg caatgctaag ttgctgtaac accgggttat
    30901 gtaccgactg cagcaccgcc tcgtggtagc gaatatccgc ttcgttaaac gcttcgcggt
    30961 cctgattgtt ggcaatcatc tcgttcagcg ccgattcaat ctgcgccaga tcgctggaag
    31021 tcgcgcgctc tgctgcccaa cgggcaatcg ccggttccac cagatttcgc acttcgctca
    31081 tggcactgat aagccgtggg tcgtagtcgt tttccagcac ccattgcagt acgtcagtgt
    31141 cgaggtaatt ccactggtta cgcggtgcca caaacgcgcc gcgataacgt ttcatttcaa
    31201 tcagccgttt cgccatcaac gaacggaaca cctcacggat gatgttgcgc gaggttgcaa
    31261 actcttcaca gagctccgcc tcagccggaa gtggcgagcc aggtacgtat ttgccgtgaa
    31321 cgatctgttt acccagcgta atgacaatgc gatcggtttt attgagagtc atggagagtc
    31381 cttgtgctct gtatgttctt ctctacttta ccccgatcga tgcataacgc ggcaactttg
    31441 tagtaccagc gtgatgacgt tcgcgtttgc cgtgtgcgta atgtagtaca aacttatatt
    31501 gttgtactac aatttagatc acaaaaagaa caatgcataa aaaaatgaca tgcgtcgggc
    31561 agaaatctga aaagggatat caggcgctaa acaggaggga aagaagagta tgctttcaac
    31621 ggcttagcta ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga ttttttaagg
    31681 ctgccggttt attcccactg gcgctgatgc tttcaggctg tatctcgtat gctctggttt
    31741 cccataccgc aaagggtagt tcaggaaagt atcaatcgca gtcagacacc atcactgggc
    31801 tatcgcaggc aaaagatagt aatggaacaa aaggctatgt ttttgtaggg gaatcgctgg
    31861 attaccttat cactgatggt gccgatgaca tcgttaagat gctcaatgat ccagcactta
    31921 accggcacaa tattcaggtt gccgatgacg caagatttgt tttaaatgcg gggaaaaaga
    31981 aatttaccgg cacaatatcg ctttactacc actggaataa cgaagaagaa aaggcactgg
    32041 caacgcatta tggttttgcc tgtggtgttc aacactgtac caggtcactg gaaaacctaa
    32101 aaggcacaat ccatgagaaa aataaaaaca tggattactc aaaggtgatg gcgttctatc
    32161 atccgtttaa agtgcgattt tatgaatact attcacccag aggcattccg gatggtgttt
    32221 ccgcagcatt actgccagtg actgttacgc tggacatcat tactgcaccg ctgcaatttc
    32281 tggttgtata tgcagtaaac caataatcag taagcgggca aacgcgttta tgctgtttgc
    32341 ccgtccactg attaattcag cacatacttc tcaatagcaa acgccacgcc atcttcaagg
    32401 ttggatttgg tgacaaagtt cgccacttct ttcaccgaag gaatagcgtt atccatcgcc
    32461 acaccgacgc ctgcatattc aatcattgcg atgtcgtttt cctgatcgcc aatcgccatg
    32521 atttcttccg gtttaatacc taacacgtcg gccagtgatt tcacccccgt acctttgtta
    32581 acgcgtttat cgaggatttc gaggaagtac ggcgcacttt tcagcacggt atatttctct
    32641 ttcacttcct gcggaatacg cgcgatagcc tggtcgagga tggcgggttc atcaatcatc
    32701 atcactttca ggaactgggt attggggtcc attttctccg cttcgcagaa caccagcgga
    32761 attgtggcaa cgaaggattc atgcaccgtg tagtagctga tatcacggtt ggcggtgtac
    32821 agcgtggtgc ggtccagggc gtggaaatga gaaccgactt cgcgagagag tttttccagg
    32881 aaacggtagt cgtcatagct gagagcggtt tgcgccacgg tgctaccatc agcggccttc
    32941 tgtaccagcg cgccgttata agtaatgcag tagtcgcccg gcagttccat atgcagctct
    33001 ttcaggtaat tgtgaacacc tgcataaggg cgacccgtcg ttagcacgac attcacgcca
    33061 cgggcgcgag ctgcggcaat cgcattttta acggcgggtg aaatggtgtg atcgggtagc
    33121 agaagggtgc catccatatc gatagcaatg agtttgatag ccatgagttc cccaggtaga
    33181 ttagttcctg acccatgcta acgcgattcc gctcaaaaat cagtacaaca cccgagggaa
    33241 aagggggatg caacgcgggt tgcatgctcc ccatttgctt agcggaagag tttccctttc
    33301 agcagttcca tgcctgcgga aagcagatcg ttattggctt gtggagacac ttcaccttgc
    33361 ggtgagagcg catcaataat cttcggcaat tgttctgcca gtaaactgga agctgtactg
    33421 gtatccacgc caagtttttg tccaagatcg gacaccgcat ttgtgccgag cgccgattcc
    33481 agttgctcgc cactaaccga ttgattgccc tgttgattac tcagccaggt tgagagaatg
    33541 gcccctaagc cgccactttg cagtttttcc agcagcacct gaatgccgcc ctgctcctca
    33601 acccaactta aaatagcctg atatttcccc gcatcgcctt tcagaaaggc accgacaact
    33661 tcatcaaaaa gccccatgat aatcacctgt aaagcgttac gtgttgaccc aaaaagtata
    33721 gatttgcggg atgataattg cggattgcag aaataaaaag ggcggagatg atctccgccc
    33781 ttttcttata gcttcttgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg
    33841 aaaattcaat acattgcaag attttcgtag gcctgataag cgtagcgcat caggcacgct
    33901 cgcatggtta gcgccattaa atatcgatat tcgccgcttt cagggcgttc tcttcaataa
    33961 acgcacggcg cggttcaacg gcgtcgccca tcagcgtggt gaacaactgg tcagcggcaa
    34021 tcgcatcctt aacggtaacg cgcagcatac gacggctttc cgggtccatg gtggtttccc
    34081 acagctgttc cgggttcatc tcgcccagac ctttataacg ctggatggag aggccgcgac
    34141 gggactcttt caccagccag tccagcgcct gctcgaagct ggctaccggc tgacgacgct
    34201 cgccacgttc gataaacgca tcttcttcca gcaagccacg cagtttctca cccagcgtgc
    34261 agatacgacg atattcgcca ccggtgataa actcgtgatc cagcggatag tcagtatcca
    34321 caccgtgggt acgcacgcga acaatcggct cgaacaggtt ttgttcggca ttggtgtgga
    34381 catcaaactt ccactggctg ccgtgctgtt ctttgtcgtt cagttcgctg accagcgcgt
    34441 tcacccagcg ggtaacggtc tgctcatcag agaggtcggc ttccgtcagc gtcggctgat
    34501 agataagctc tttcagcatt gctttcggat aacgacgctc catgcggttg atcattttct
    34561 gcgtcgcgtt gtactcagac accaatttct ctaacgcttc gccagccaat gccggtgcgc
    34621 tggcgttggt gtgcagcgtt gcgccatcca gcgcgataga gatctggtac tgatccatcg
    34681 cttcgtcgtc tttaatgtac tgttcctgct tgcctttctt cactttgtac agcggcggct
    34741 gagcgatgta gacgtgaccg cgttcaacga tttccggcat ctgacgatag aagaaggtca
    34801 acagcagcgt acgaatgtgc gagccgtcga cgtccgcatc ggtcatgatg atgatgctgt
    34861 gataacgcag tttgtccggg ttgtactcgt cacgaccgat accacagcca agcgcggtga
    34921 taagcgtcgc cacttcctga gaagagagca tcttatcgaa gcgcgctttc tcgacgttga
    34981 ggattttacc cttcagcggc agaatcgcct ggttcttgcg gttacgcccc tgcttcgcag
    35041 agccgcccgc ggagtcccct tccaccaggt acagttcgga aagcgccgga tcgcgttcct
    35101 ggcagtctgc cagtttgccc ggcaggcccg ctaagtcgag cgcaccttta cggcgggtca
    35161 tttcacgcgc gcgacgcgcc gcttcacggg cacgggcagc atcgataatt ttgccaacca
    35221 cgattttcgc gtcggttggg ttttccagca ggtattctgc cagcagttcg ttcatctgct
    35281 gttcaaccgc tgatttcacc tcagaagaaa ccagtttgtc tttggtctgg gaggagaatt
    35341 tcgggtccgg cactttcacg gaaacgaccg caatcaggcc ttcacgcgca tcgtcaccgg
    35401 tggcgctaac tttggctttt ttgctgtagc cttctttgtc catgtaggcg ttcagggtac
    35461 gggtcatcgc cgcacggaag cctgccaggt gagtaccgcc gtcacgctgc ggaatgttgt
    35521 tggtaaagca gtagatgttt tcctggaagc catcgttcca ctgcaacgcc acttcgacgc
    35581 caataccgtc tttttcagtg gagaagtaga agatattcgg gtggatcggc gttttgttct
    35641 tgttcagata ttcaacgaac gccttgatgc cgccttcata gtggaagtgg tcttctttgc
    35701 cgtcgcgctt gtcgcgcaga cgaatggaaa cgccggagtt gaggaacgac aactcacgca
    35761 gacgtttcgc cagaatttca tattcgaact cggtcacatt ggtgaaagtt tcgaggctag
    35821 gccagaaacg caccatggtg ccggtttttt cagtctcgcc ggtaaccgcc agcggggcct
    35881 gcggtacacc gtgttcgtag atctgacggt gaattttacc ctcgcgctgg ataaccagct
    35941 ccagtttttg cgacagggcg tttactaccg aaacaccaac gccgtgcaga ccgccggaca
    36001 ctttatagga gttatcgtcg aatttaccgc ctgcgtgcag aacggtcatg atcacttccg
    36061 ccgccgatac gccctcttcc gggtgaatac cggtcggaat gccgcgcccg tcatcctgta
    36121 cagagacaga gttgtcggcg tgaatggtga cgataatttc tttacagtga cccgcgagcg
    36181 cttcgtcgat agcgttatct accacctcga ataccatgtg gtgcagaccg gtgccgtcat
    36241 ccgtgtcgcc gatatacata cccgggcgct tacgcaccgc atccagccct ttcaggactt
    36301 tgatactgga ggagtcataa gaattcgaca tcaacgtttc tcgctcattt atacttgggt
    36361 taatccgtta ttttaccctt ttccacggta aacatcttcg aattttcgtc cgacatgtct
    36421 ataacgtgtt cagcactgat cgcgctgaca aagacctgtg attgcgtcgc ttttaagcgg
    36481 ctggcaagca acccacgacg ctcatcatca agctcagagg caaaatcatc tatcaggtag
    36541 agacaccgcc gcccgctttc acgggtgagg aactctcctt gcgccagacg taaggcgcac
    36601 atcaacagct taagctgccc acgcgataag gtatcttcca ccggcgcacc gtcggcgcga
    36661 atgcgtaagt ccgctttatg cgggccatgc gcggtgtagg ttagctggcg atcgcgttca
    36721 aaattacgtt ccagcacctc agcatattct gtctctttct cccagccgcg ctggaaagag
    36781 aaagtcagag aaaactcagg gagaaattgc ttacaggtat cggccatatc ggccgcgata
    36841 ccggcgctat actccgcgcg ccaggtgctg atttgctccg ccagcgggat cagttcttta
    36901 tcccacgggc gtagctgttc gtaacgtgtc acctggcgca gcgccgcatt gcgctgcttg
    36961 agcaatcgct tgagattgct ccaggcggtg aaaaatccgg gttcgttgtg aaagcatccc
    37021 cagtcgagga atgctcttct gtatttgggg ccgccgttga gtaaagtaaa cccttctggc
    37081 gttatcagct gcattggcat caggtgcgcc agttccgcga ctttatgccc gtcagtaccg
    37141 tcgatgcgga ctttgctgtc gccctgtttg tccttggtta agccaatcgc cgtctcgcgc
    37201 tcttcgccct gtaatcgccc atggagaaca aatgcctcct gctcatggcg aatgacgcga
    37261 ccaatctgca aactgcgaaa cgcccgacca tggccgagcg tatagatggc ttccagcacg
    37321 ctggttttgc cactgccgtt ggcacctacc agaaagttaa agccgggaga taaagcgaga
    37381 tccgcggttt caatgttgcg gaaatcgcgg atcaacaagc gggtgaggga cattacagtc
    37441 tcattggcat gacaacatag gcagccgact gtgatgcggc atcttcaatc tgcacgctgg
    37501 aaaccgaatc ggtcagcatc atgcggacgt tttcgcattt cagcgcgttc agaacatcca
    37561 gcacatagct gacgttgaag ccgatttcca tctccgcacc gctataggta acgtcgagga
    37621 tctcttccgc ttcttcctgt tccgggttgt tggcggtgat tttcagctgg ttttcgctga
    37681 catacaggcg cacgccgcgg aatttctcgt tagagagaat tgccgcacgg gcaaacgcct
    37741 gcttgagcag atcgcagcca gcttccagat gtttgtccgg attcttcggc agaacgcggc
    37801 ggtaatccgg gaagcgacca tccaccagtt tggaggtgaa gataaagtcg ccaacgtggg
    37861 cgcgaatatt gttgctgcca atctgcacgc gcagcggatt gtcgccgccg tcgagcatac
    37921 gcatcagttc aatcacgcct ttacgcggta cgatcaccga atggcttggc aaagattgac
    37981 caattggcat tgaacagacc gccagacggt ggccgtcggt tgccacggtg cgcagttctt
    38041 caccttcggt ttcaaacagc ataccattta agtaatagcg aacgtcctga tgcgccatcg
    38101 aaaactgggt cgcttcaatc agacgcttca tcgttgcctg cggcagggta aattcgactt
    38161 cactctgcca gtcatcgagg ttcgggaaat ccgccgctgg cagggtagac agcgaaaaac
    38221 ggctacgccc ggagcgtacc agcatccgtt caccttccag ctgcacggca atttccgcgc
    38281 cttcaggcag accacggcag atatcaaaga atttgcgcgc cggaacggtc gtcgctcctg
    38341 gctcgtgtgg ctgaaccagc gcaacacgtg ccaccatttc catctcgaga tcagtaccgg
    38401 tcagcgacaa cgtaccgtca gcaacctgta acagcagatt accgagaatc ggtagcgtag
    38461 gacgaccacc taacggaccg ctcacctgtt gtagcggttt taataaatgc tcacgttcta
    38521 cggtaaattt cataggttta cgatgacaat gttctgatta aatttgaaaa atcttctttg
    38581 atatcgtggc tctcttcacg caactgctcg atcttacggc aggcatgaag caccgtcgtg
    38641 tggtcacgac caccaaacgc atcgccaatc tccggcagac tgtggttagt cagctctttc
    38701 gccagcgcca tcgccatctg gcgcggacgc gccaccgagc gggatcgacg cttggaaagg
    38761 agatccgcga ctttgatctt gtagtactcc gccaccgtct tctgaatatt gtcgatggtg
    38821 accagttttt cctgcaatgc cagcaagtcg cgcagcgcct cacgcacgaa gtcgatggtg
    38881 atcgcccgtc cggtaaagtt ggcattggca atgacgcggt tcagcgcccc ttccagctca
    38941 cgtacgttag atcgtagacg cttggcgata aagaacgcca cttcgcccgg caaacgaatg
    39001 tcgttttcgt cggccttttt catcaggatc gccacacggg tttccagctc tggcggttcg
    39061 attgccacag tcagtcccca accgaagcgg gatttcaaac gatcctcaac gccgttgatc
    39121 tctttcggat agcgatccga ggtgagaatg atctgttgat taccttccag cagggcgttg
    39181 aaggtatgga aaaactcttc ctgagatcgt tctttattag caaaaaactg aatatcgtcg
    39241 atcagcagtg catctacgga acggtagtag cgtttaaact cttcgatcgc gttgttttgc
    39301 agggctttaa ccatgtcctg aacaaagcgc tcggagtgca tataaaccac tttggcattc
    39361 ggcttgcgcg ccataatgcc gttacccacc gcatgcagca ggtgagtttt acccagaccc
    39421 gtgccgccat aaaggaacaa cgggttatag gcaccgccag ggttatccgc cacctggcga
    39481 gccgccgcgc gcgccagttg gttagattta ccttcaacga agttatcaaa cgtgtgtttg
    39541 acgtttacgt tagaacgata ggtcggttct gccggggccg ggatgttatc ccaacctgag
    39601 cgcgtagaag gcgcagcacg ttgcggctgc gtttgcgcca cctgtgcagg ggccgcgacg
    39661 ttgctcgtca ctgccgcttg tggcgtttgc gtcaccggtt tggtgccgac ttcaaaacgc
    39721 agctgtgggg catccgctcc gcagaaactg gttagcagtc cattgatatt attaaggtac
    39781 ttgtcccgta cccaatcgag gacaaaacgg tttggcgcgt acagggccag cgtgttatcg
    39841 ctcagttccg cctgcaatgg gcgtatccac atactgaatt ctgtggctgg taactcatcc
    39901 tgcaatcggg caagacactg ctgccaaagc gaaagtgaca cggcggactc cactcgaaca
    39961 aaagtcgata atgactaagg ctgaaacatt catgattgtt gacgtacgtc gaaaagaccc
    40021 tgcttgaggg tgacgcacga accgctgtct gcggttatat gcccgatcaa gatcctgcaa
    40081 aacgatcggg accgcggatc atagcctaaa ctgcgcaaga gatcttctgt ttctcacaga
    40141 ttttcccgat ttatccacag gactttccag acctcgctaa gtgtaaacga tcctgccgcg
    40201 aggcgggcac gatttacgcc gcatattgga aaatttaatg accatagaca aaaattggct
    40261 taatcgatct aataaagatc caggacgatc cttgcgcttt acccatcagc ccgtataatc
    40321 ctccacccgg cgcgccatgc tggttttcac tggtgtgagg tcgtacattt tccctgcgaa
    40381 aaggtgcgga aaagcgcggt aaataaggaa agagaattga ctccggagtg tacaattatt
    40441 acaatccggc ctctttaatc acccatggct tcggtgtcca tcgtttcatt tttcggcgga
    40501 tatccaataa agccattgaa tttattcaag tttaggtaga aatcgccatg aaacgcactt
    40561 ttcaaccgtc tgtactgaag cgcaaccgtt ctcacggctt ccgtgctcgt atggctacta
    40621 aaaatggtcg tcaggttctg gcacgtcgtc gtgctaaagg ccgcgctcgt ctgaccgttt
    40681 ctaagtaata aagctaaccc ctgagtggtt aagctcgcat ttcccaggga gttacgcttg
    40741 ttaactccca gtcaattcac attcgtcttc cagcagccac aacgggctgg cacgccgcaa
    40801 attaccattc tcggccgcct gaattcgctg gggcatcccc gtatcggtct tacagtcgcc
    40861 aagaaaaacg ttcgacgcgc ccatgaacgc aatcggatta aacgtctgac gcgtgaaagc
    40921 ttccgtctgc gccaacatga actcccggct atggatttcg tggtggtggc gaaaaaaggg
    40981 gttgccgacc tcgataaccg tgctctctcg gaagcgttgg aaaaattatg gcgccgccac
    41041 tgtcgcctgg ctcgcgggtc ctgatagccc tcattcgggt ctatcaacgc ctgattagtc
    41101 cgctactcgg gccgcattgt cgtttcactc caacctgttc aagctacgga attgaggcat
    41161 tgcgcaggtt tggagtgata aaaggcagtt ggttgacggt gaaacgcgta ttaaaatgcc
    41221 accctttaca ccctggtggt gacgatcccg tcccgcccgg accatttgat accagagaac
    41281 actaacgatg gattcgcaac gcaatctttt agtcatcgct ttgctgttcg tgtctttcat
    41341 gatctggcaa gcctgggagc aggataaaaa cccgcaacct caggcccaac agaccacgca
    41401 gacaacgacc accgcagcgg gtagcgccgc cgaccagggc gtaccggcca gtggccaggg
    41461 gaaactgatc tcggttaaga ccgacgtgct tgatctgacc atcaacaccc gtggtggtga
    41521 tgttgagcaa gctctgctgc ctgcttaccc gaaagagctg aactctaccc agccgttcca
    41581 gctgctggaa acttcaccgc agtttattta tcaggcacag agcggtctga ccggtcgtga
    41641 tggcccggat aacccggcta acggcccgcg tccgctgtat aacgttgaaa aagacgctta
    41701 tgtgctggct gaaggtcaaa acgaactgca ggtgccgatg acgtataccg acgcggcagg
    41761 caacacgttt accaaaacgt ttgtcctgaa acgtggtgat tacgctgtca acgtcaacta
    41821 caacgtgcag aacgctggcg agaaaccgct ggaaatctcc acctttggtc agttgaagca
    41881 atccatcact ctgccaccgc atctcgatac cggaagcagc aacttcgcac tgcacacctt
    41941 ccgtggcgcg gcgtactcca cgcctgacga gaagtatgag aaatacaagt tcgataccat
    42001 tgccgataac gaaaacctga acatctcttc gaaaggtggt tgggtggcga tgctgcaaca
    42061 gtatttcgcg acggcgtgga tcccgcataa cgacggtacc aacaacttct ataccgctaa
    42121 tctgggtaac ggcatcgccg ctatcggcta taaatctcag ccggtactgg ttcagcctgg
    42181 tcagactggc gcgatgaaca gcaccctgtg ggttggcccg gaaatccagg acaaaatggc
    42241 agctgttgct ccgcacctgg atctgaccgt tgattacggt tggttgtggt tcatctctca
    42301 gccgctgttc aaactgctga aatggatcca tagctttgtg ggtaactggg gcttctccat
    42361 tatcatcatc acctttatcg ttcgtggcat catgtacccg ctgaccaaag cgcagtacac
    42421 ctccatggcg aagatgcgta tgctgcagcc gaagattcag gcaatgcgtg agcgtctggg
    42481 cgatgacaaa cagcgtatca gccaggaaat gatggcgctg tacaaagctg agaaggttaa
    42541 cccgctgggc ggctgcttcc cgctgctgat ccagatgcca atcttcctgg cgttgtacta
    42601 catgctgatg ggttccgttg aactgcgtca ggcaccgttt gcactgtgga tccacgacct
    42661 gtcggcacag gacccgtact acatcctgcc gatcctgatg ggcgtaacga tgttcttcat
    42721 tcagaagatg tcgccgacca cagtgaccga cccgatgcag cagaagatca tgacctttat
    42781 gccggtcatc ttcaccgtgt tcttcctgtg gttcccgtca ggtctggtgc tgtactatat
    42841 cgtcagcaac ctggtaacca ttattcagca gcagctgatt taccgtggtc tggaaaaacg
    42901 tggcctgcat agccgcgaga agaaaaaatc ctgattcggt gagttttcgc taaaataagg
    42961 gcggtcagtt gaccgccttt tttcttttcg tagggcggat aagcaccgcg catccgccac
    43021 acaaagcaac aggaacatca tgagcgataa tgacactatc gtagcccagg ccacgcctcc
    43081 gggacgtggc ggtgttggca tcctgcgcat ctccggcctc aaagcccgtg aagttgccga
    43141 aaccgtgctg ggtaaactac ctaagccgcg ctacgccgat tatttgccat ttaaagacgc
    43201 cgacggcagc gtgctcgatc aggggattgc gctatggttc cctggcccga actcgttcac
    43261 cggcgaagat gtgcttgaac tgcagggtca tggcggtccg gtgatcctcg acctgctgtt
    43321 aaaacgcatt ctgaccattc ccggcctgcg gattgctcgc cctggtgagt tttccgaacg
    43381 cgcgtttctt tacgataaac tcgacttagc ccaggctgag gcgattgccg atcttatcga
    43441 cgccagctcg gaacaggcgg cccgttcggc acttaactcg ctgcaaggcg cattctccgc
    43501 acgggttaat catctggtag aagccctcac ccacttgcgc atttacgtcg aagcggcaat
    43561 tgatttccct gatgaagaga tcgatttcct ctccgacggc aaaattgaag cccagctcaa
    43621 tgacgttatt gccgatcttg atgcagtgcg tgctgaagca cgtcagggta gtttgttgcg
    43681 cgaagggatg aaagtggtga ttgccggacg tcctaacgcc ggtaaatcga gcctgttaaa
    43741 cgcgctggct ggccgtgaag cagcaatcgt aaccgatatc gccggaacta cgcgtgacgt
    43801 gttgcgtgag catatccaca ttgacggaat gccgctgcat atcatcgaca ccgccgggct
    43861 acgtgaagcc agtgacgaag tggaacgtat tgggatcgag cgcgcgtggc aggaaattga
    43921 acaggccgac cgggtgctgt ttatggtcga tggcaccaca acagacgccg tggatccggc
    43981 agagatctgg ccggaattta tcgcccgtct gccagcgaaa ctgccgatca ccgtggtgcg
    44041 caataaagcc gatatcaccg gcgaaacgct gggaatgagt gaagtgaacg gtcacgcgtt
    44101 aattcgtctc tcggcaagga ctggtgaagg cgtggacgtg ctgcgtaacc atctcaaaca
    44161 gagcatgggc tttgacacca acatggaagg cggtttcctg gcgcgccgtc gccacttaca
    44221 ggcgctggaa caggcagcgg agcatttgca gcaaggtaaa gcgcaactgt taggtgcctg
    44281 ggcgggtgaa ctgctggcgg aagagttgcg cctggcacag cagaacttaa gcgaaatcac
    44341 cggggaattt acttcagacg acctgctggg gcggattttc tccagcttct gtattggtaa
    44401 gtaaccgcgc ttacgaagcc gtattctgac tgtcagatgc ggcttcgctt cattgttagc
    44461 actcctgtta ttcctcaacc ctttttttaa acattaaaat tcttacgtaa tttataatct
    44521 taaaaaaaag catttaatat tgctccccga acgattgtga ttcgattcac atttaaacaa
    44581 ttttagaata gacaaaattc ttgagtatta tactgtagcc tcgtgtcttt cgaggataag
    44641 tgcattatga atatcttaca tatatgtgtg acctcaaaat ggttcaatat cgacaataag
    44701 attgtcgatc accgccctta atttaccctt ctgtagccat caccagagcc aaaccgatta
    44761 gattcaatgt gaacgattag tttgctatat cttaattttg ccttttgcaa aggccatctc
    44821 tcgtttattt acttatttta gtaaatgatg gtgcttgtat atatacatgg cgaattaatg
    44881 gatattgcag atgtaatatt cacagggatc actgtaatta aaataaatga aggattatgt
    44941 aatggaaaac tttaaacatc tccctgaacc gttccgcatt cgtgttattg agccagtaaa
    45001 acgtaccact cgcgcttatc gtgaagaggc aattattaaa tccggtatga acccattcct
    45061 gctggatagc gaagatgtgt ttatcgattt actgaccgac agcggcaccg gggcggtgac
    45121 gcagagtatg caggccgcga tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta
    45181 ctatgcgtta gccgagtcag tgaaaaatat ctttggttat caatacacca ttccgactca
    45241 ccagggccgt ggcgcagagc aaatttatat tccggtactg attaaaaaac gcgagcagga
    45301 aaaaggcctg gatcgcagca aaatggtggc attctctaac tatttctttg ataccacgca
    45361 gggccatagc cagattaacg gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga
    45421 tactggcgtg cgttacgact ttaaaggtaa ctttgacctt gagggattag aacgcggtat
    45481 tgaagaagtt ggcccgaata acgtgccgta tatcgttgca accatcacca gtaactctgc
    45541 aggtggtcag ccggtttcac tggcaaactt aaaagcgatg tacagcatcg cgaagaaata
    45601 cgatattccg gtggtaatgg actccgcacg ctttgctgaa aacgcctatt tcatcaagca
    45661 gcgtgaagca gaatacaaag actggaccat cgagcagatc acccgcgaaa cctacaaata
    45721 tgccgatatg ctggcgatgt ccgccaagaa agatgcgatg gtaccgatgg gcggcttgct
    45781 gtgcatgaaa gacgacagct tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt
    45841 gcaggaaggg ttcccgacat atggcggcct ggaaggcggc gcgatggagc gtctggcggt
    45901 aggtctgtat gacggcatga gtctcgactg gctggcttat cgtatcgcgc aggtacagta
    45961 tctggtcgat ggtctggaag agattggcgt tgtctgccag caggcgggcg gtcacgcggc
    46021 attcgttgat gccggtaaac tgctgccgca tatcccggca gaccagttcc cggcacaggc
    46081 gctggcgtgc gagctgtata aagtcgccgg tatccgtgcg gtagaaattg gctctttcct
    46141 gttaggccgc gatccgaaaa ccggtaaaca actgccatgc ccggctgaac tgctgcgttt
    46201 aaccattccg cgcgcaacat atactcaaac acatatggac ttcattattg aagcctttaa
    46261 acatgtgaaa gagaacgcgg cgaatattaa aggattaacc tttacctacg aaccaaaagt
    46321 attgcgtcac ttcaccgcaa aactgaaaga agtttaatta atactacaga gtggctataa
    46381 ggatgttagc cactctctta ccctacatcc tcaataacaa aaatagcctt cctctaaagg
    46441 tggcatcatg actgatcaag ctgaaaaaaa gcactctgca ttttggggtg ttatggttat
    46501 agcaggtaca gtaattggtg gaggtatgtt tgctttacct gttgatcttg ccggtgcctg
    46561 gtttttctgg ggtgccttta tccttatcat tgcctggttt tcaatgcttc attccgggtt
    46621 attgttatta gaagcaaatt taaattatcc tgtcggctcc agttttaaca ccatcaccaa
    46681 agatttaatc ggtaacacct ggaacattat cagcggtatt accgttgcct tcgttctcta
    46741 tatcctcact tatgcctata tctctgctaa tggtgcgatc attagtgaaa cgatatcaat
    46801 gaatttgggc tatcacgcta atccacgtat tgtcgggatc tgcacagcca ttttcgttgc
    46861 cagcgtattg tggataagtt cgttagccgc cagtcgcatt acctcattgt tcctcgggct
    46921 gaagattatc tcctttgtga tcgtgtttgg ttctttcttc ttccatgtcg attactccat
    46981 cctgcgcgat gccaccagca ccactgcggg aacgtcttac ttcccgtata tctttatggc
    47041 tttgccggtg tgtctggcgt catttggttt ccacggcaat attcccagcc tgattatttg
    47101 ctatggaaaa cgcaaagata agttaatcaa aagcgtggta tttggttcgc tgctggcgct
    47161 ggtgatttat ctcttctggc tctattgcac gatggggaat attccgcgcg aaagctttaa
    47221 ggcgataatc tcctcaggcg gcaacgttga ttcgctggtg aaatcgttcc tcggcaccaa
    47281 acagcacggc attatcgagt tttgcctgct ggtgttctct aacttagctg ttgccagttc
    47341 gttctttggt gtcacgctgg ggttgttcga ttatctggcg gacctgttta agattgataa
    47401 ctcccacggc gggcgtttca aaaccgtgct gttaaccttc ctgccacctg cgttgttgta
    47461 tctgatcttc ccgaacggct ttatttacgg gatcggcggt gccgggctgt gcgccaccat
    47521 ctgggcggtc attattcccg cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca
    47581 gatgttcacg gtctggggcg gcaatcttat tccggcgatt gtcattctct ttggtataac
    47641 cgtgattttg tgctggttcg gcaacgtctt taacgtgtta cctaaatttg gctaaatcct
    47701 tcaagaagcc agccattcgc tggcttcttg cctctcagga aatcacttat gtccaaatgg
    47761 caactcgcct gattctcctt caccacgtat gctttgcgtc accttactat caggacgctt
    47821 tagcccatgt cccgcttttt aatttgtagt tttgccctgg ttttacttta tcccgccggg
    47881 attgatatgt acctcgttgg tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg
    47941 cagttgcata ttgcgttctc cgtttatctg gcgggcatgg cagctgcgat gttatttgcc
    48001 ggtaaagtgg ccgatcgttc agggagaaag cctgtcgcca tccccggcgc ggcgctattt
    48061 attatcgctt cggtgttctg ttcactggct gaaaccagca cgttatttct tgcagggcgc
    48121 tttctacagg ggttgggcgc aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac
    48181 acgctggatg atcgacgtcg ggctaaagtg ctgtcgttac tcaacggtat tacctgcatc
    48241 attccggtgt tagcgccggt gctcggacac ctgattatgc ttaaattccc ttggcagagt
    48301 ctgttctgga cgatggcaat gatgggcatc gcggtactga tgttatcttt gtttatttta
    48361 aaagaaacgc gcccagcgtc ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg
    48421 ctgcttaatc ggtttttcct cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc
    48481 ctcactttcg tcaatacatc gccggtattg ctgatggaaa tcatgggttt tgagcgcgga
    48541 gaatacgcca ccattatggc gttgaccgct ggcgtcagca tgaccgtttc attctccacg
    48601 ccatttgcgc tgggaatttt taagccacgt acgttgatga tcacctcgca ggtgttattc
    48661 cttgcggcgg ggatcaccct tgccgtttca ccttcccatg cggtttctct gtttggtatc
    48721 acgctgattt gcgccggttt ctcggtaggt tttggcgtag cgatgagtca ggcgttagga
    48781 ccattttcat tacgcgcggg cgtagccagc tcgaccttag gtattgcgca ggtttgcggt
    48841 tcgtcactgt ggatttggct ggcagcggtg gttggtatcg gcgcatggaa tatgctgatc
    48901 gggattctga ttgcctgtag catagtgagc ctgttactga ttatgttcgt cgcgcctgga
    48961 cgccccgttg ccgctcatga agaaatccat caccacgctt gatctcaatc tgctgctctg
    49021 tctgcaactg ctgatgcagg agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac
    49081 cccttcagcg gtgagtaagt cgctggcaaa gttaagagcg tggtttgacg acccgctctt
    49141 tgtgaactca ccgctggggc tgtcgcccac accgctaatg gtcagcatgg agcaaaatct
    49201 ggcggaatgg atgcaaatga gcaatctgct gctggataaa ccgcaccacc agacaccgcg
    49261 cggcctgaag tttgagttgg cggcggaatc gccgctgatg atgatcatgc ttaatgcgct
    49321 gtcgaaacgg atctaccaac gttacccgca ggcgaccatc aaactacgta actgggatta
    49381 cgattcctta gatgccatta ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag
    49441 ccatcctcgc tcgcgggagc tgttaagctc gctaccgtta gccattgatt atgaagtgct
    49501 gtttagtgat gtaccctgcg tctggttacg caaagatcat ccggcactgc atgaaacgtg
    49561 gaatctggac acctttttac gttatccgca tatcagcatt tgctgggaac agagcgacac
    49621 ctgggcgctg gacaatgtgt tacaggagct ggggcgcgaa cgcacgattg ctatgagcct
    49681 gccggaattt gagcagtcac tgtttatggc agcgcaaccc gacaatctgc tactggcgac
    49741 cgcgccgcgc tactgtcagt actacaatca actccatcaa ctgccgttgg ttgctcttcc
    49801 tctcccgttt gacgaaagcc agcaaaaaaa gctggaagtt ccttttaccc tgctgtggca
    49861 taaacggaac agccataatc cgaagatcgt ctggttacgg gaaaccatta aaaaccttta
    49921 cgcgtcgatg gcataaccga atcgtatgaa acgcgaccca atttcacaat aaaatgtaaa
    49981 aaagttgtaa taagcttgtc tgaatcgaac ttttagccgc tttagtctgt ccatcattcc
    50041 agtaaatgat tactcttata ttcataatgg accattaagc atggagcgaa aaatggcgac
    50101 tcactttgcc cgagggattt taacggaagg acatctgatt tctgttcgtc tcccctccca
    50161 gtgtcatcaa gaagcccgaa acattccccc tcatcgtcaa agccgttttc tggcgtccag
    50221 aggtttactc gcagaactga tgttcatgct gtatggcatt ggcgaattgc cggaaatcgt
    50281 caccctgccg aaaggtaaac cggttttcag tgataaaaat ttgccttcgt tttctatttc
    50341 ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa ggtgaatgtg gcctcgatat
    50401 ggaactacag cgtgcgacgc gcgggtttca tagcccacac gcgcccgata accacacctt
    50461 ttccagcaat gaatcgctat ggatcagtaa acaaaacgat cctaacgaag cgcgggcgca
    50521 gctcatcacg ctgcgccgaa gcgtgctaaa actaaccggt gatgttttga atgacgatcc
    50581 gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa tgtgctcatg taaatcatgt
    50641 agaagcgtta tgcgacgcgg aggacgtgct agtgtggtcc gtggcggtca cgcccgcgat
    50701 tgaaaagctc agtgtctggg agttagatgg caaacacagc tggaaaagcc tgccggatat
    50761 tcacagccgc gccaacaatc ctaccagccg gatgatgcgt tttgcccagc tctctaccgt
    50821 gaagtctttt tcgccaaatt gatagacaac cacaggagtc attatgtctg aaaaattgca
    50881 ggtggttacg ttactgggga gcctgcgcaa aggctcattt aatggcatgg ttgcacgtac
    50941 cctgccgaaa attgctccgg cgagcatgga agtcaatgcg ttaccttcca ttgccgacat
    51001 tcccctgtat gacgctgacg tacagcagga agaaggtttt ccagcaacgg ttgaagctct
    51061 ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc acgccggaat ataactactc
    51121 ggtaccgggt gggctgaaaa atgccatcga ctggctttcc cgcctgccgg atcaaccgct
    51181 ggccggtaaa ccggtgttga ttcagaccag ctcaatgggc gtgattggcg gcgcgcgctg
    51241 tcagtatcac ctgcgccaga ttctggtttt cctcgatgca atggtgatga acaagccgga
    51301 atttatgggc ggcgtgattc agaacaaagt tgatccgcaa accggagaag tgattgatca
    51361 gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta ttcagcgagt
    51421 taagatctaa ataaaaaacc cgccagcaat catgcatggc gggtttttaa cgcgctatcg
    51481 attttagtga gcgtcgataa agacaatctt caggataaac agcagcgcaa cgatgattac
    51541 gcacgggctt agatcacgca gacgtccggt accgattttc atcacgcagt aggagataaa
    51601 gcccagcgcg ataccttcgg taatcgagaa gctgaacggc atcatcacgg cggtaataaa
    51661 cgccggaaca gattcagtaa gatcctgcca gttcacgcgt gccagactgg aagtcatcag
    51721 cacgccaacg taaatcagcg caccagctgc agcgtagcct ggcaccatcc ccgccagcgg
    51781 cgacagaaag ataaccagca ggaacagcag accaacaacc actgccgtca gaccggtacg
    51841 accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag aagtaccgat
    51901 aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca tgcgcgggaa
    51961 tttacccttc tcatccgcca ggcctgcttt atcggtcacg ccaatcagcg taccggagga
    52021 gtcaaacagg ttgaccagca tgaaagagaa aatcacccct gccagcccga ggttaaacga
    52081 cccggctaaa tctacatgac ccacaactgt cattacgctc ggcggcgcag aaacgatgcc
    52141 attgtagtgc acatcaccca gcatccagcc cagcagcgtc gtcaccacga tagaaaccag
    52201 caccgctgcg tgaatgttgc gcgaggccag aatagcaatg atgaagaagc cgaggatacc
    52261 cagaagtacg ctgtgagaag tcagattacc gatgctcacc agcgtttccg ggttagcgac
    52321 aatcacacct gcgtttttca gccccatcat gccaatgaac agaccgatac cgctggtaat
    52381 acccacacgc agactcaccg gaatgttggc aatcatccag tagcgaacgc ggaaaatcgt
    52441 cagtaacagc agacctatcg cgccccagaa gattgcgccc atcccgacct gccacggcaa
    52501 gcccatcgcc tgtacaacga caaaagcgaa gaacgcattc aggcccatag cgggtgccag
    52561 tgcaactggc aggttagcaa acagtcccat cataatactg ccgaatgcag cgatcagaca
    52621 ggtagtgacg aagacggcgc tggtatccat gccagcaacg ccaagaattt gcgggttaac
    52681 aaaaacgatg taaaccatcg tcaggaaggt ggtaaaaccg gcgatcactt cggtccgtgc
    52741 cgtcgtgcca tgttcgcgca gtttaaacac gcgttccagc atcccctggc cagaagcctg
    52801 ggttgtgtgt tgttgactca ttatctattt ccgaacaagg agggaaaatc cgtcgctatc
    52861 gtataccaaa atgagacaat atggacggct gaagataact ttttttcatt gtatttgctt
    52921 atacggcaac gattgcgtca tgcgaattgg catgacgaaa acgttaaact aacgataaag
    52981 gaaagacatg tcccagatag aagcggtatt tttcgactgc gacggtacgc tggtcgacag
    53041 tgaagtcatt tgctcccgcg catatgtaac gatgtttcag gaatttggta ttacgctcga
    53101 tcctgaagag gtattcaaac gtttcaaagg tgtaaaactg tacgaaatta tcgatattgt
    53161 ttcccttgaa catggtgtta cgttagcgaa aacagaagct gaacacgttt accgtgcaga
    53221 agtcgctcgg ctgttcgatt cagaactgga agccatcgaa ggggctggag cgctcctgtc
    53281 agcgatcact gcgccaatgt gtgtggtatc taacggccca aataacaaaa tgcagcattc
    53341 tatgggcaag ctgaatatgt tgcactactt cccggataaa ctgttcagcg gctacgatat
    53401 tcagcgctgg aagccagacc cggcgttaat gttccatgcg gcaaaagcga tgaatgtaaa
    53461 tgtagaaaac tgcattctgg ttgatgactc agttgccggt gcgcaatctg gtatcgacgc
    53521 aggtatggaa gtgttctact tctgcgccga cccgcacaat aagccgatcg ttcacccgaa
    53581 agtcaccacc tttacccatc tttcgcagtt accggaactg tggaaagcgc gtggttggga
    53641 tattacggca tagttcttca cactcccttc acttaccccg cttaaattgg cgctcaaagg
    53701 taagtaaagg gagtttgata tgtctgtttc acgtcgggta atacatcacg gactttattt
    53761 tgcggtttta ggaccgttaa ttggtgttct gtttcttgtc ctctacatat tcttcgcaaa
    53821 agaaccgctg gttcttttgc gaagaataca ggtacttcca ttgtttctct tattgtcgat
    53881 tactacggga gttattcctg cattgttaac cggcgtgatg gtcgcctgtc tgccggaaaa
    53941 gatcgggtca cagaaaaatt atcgttgtct ggctggtggc ataggtggcg tcgttatcac
    54001 cgagatctat tgtgcagtta ttgtacatat taagggcatg gcttcctcgg agttgtatga
    54061 aaacattctt tctggtgaca gtctcgttgt ccgcatcatt cctgcattgc tggcaggtgt
    54121 ggtgatgagc agaatcatta cccgtctgcc cggattggat atttcatgtc ctgaaacaga
    54181 ctctttaagt taagcgggat actttatctt tgggctactc aaaagcagac aggatgttac
    54241 tatgactcaa aatatcaggc cgttacccca attcaaatat catcccaagc cactggaaac
    54301 aggcgcattt gaacaggata aaaccgtaga gtgcgattgc tgtgaacaac agacgtcagt
    54361 ttattactcg ggtccctttt attgcgttga tgaagttgaa catctctgtc cgtggtgtat
    54421 tgcggacggt tctgctgctg aaaagtttgc aggtagtttt caggatgatg ccagcataga
    54481 aggtgttgaa tttgagtatg atgaagagga cgaatttgcc ggtattaaga acacatatcc
    54541 tgatgaaatg ctgaaagagt tggttgaacg cacgccaggt tatcatggat ggcaacagga
    54601 attctggctt gcgcattgtg gcgatttctg tgcttttatc ggctatgtgg gctggaatga
    54661 tataaaagat cgcctcgatg aatttgccaa ccttgaagaa gattgtgaga atttcggtat
    54721 tagaaattct gatctagcta aatgcctgca aaagggtggt gattgtcagg gttatctctt
    54781 ccgctgtctc cactgcggca agctgagact gtggggtgat ttttcgtagt tatttaaata
    54841 atgagaacag gccggagcgt aattcataca tccggcctta tttcttcagc ttaatcgagc
    54901 gctaattctg cggcagcggc tttatcagcg attaccatca gcgaaggatg cagctgcaac
    54961 acggaagctg gaacatcttc ggtaaccgga ccttgcagca catttttcag tgcctgcgct
    55021 ttacctgcgc cactgacaat tatcagcaag tttttcgccg ccatgatgct tttcggcccc
    55081 atcgtcacgt agctgtcagg cactaacgaa aagtcgccgc ccaactcacc atgcgccaca
    55141 atatcaacca tctctccctg gatcgggaac tccacggttt gctcatggaa atgggtcgta
    55201 ttcggcaaat tgccacaaaa atgaccatct gcacctaatc ccagcaccac caaatccagg
    55261 ccgccttcac gcgccagttt ctgatcatgc tcgcggtagt tatcaatagt gagcttctgg
    55321 atattctctt ctttgatccc cgcaggggtg aaaaacagat tacgcagatt ggtaatcgtt
    55381 acaccttctc cctctttgcc gcgaaaggga atttcatcga aattatagaa atagcagtta
    55441 tcataccagg gcttaccttt aaccagggtg gtgaggtatt catacatgcc tttgggcgtg
    55501 ctaccggcgg taattgccag gttaacacga cgcgtcttcg acatataacc aagcagatgg
    55561 tgtgccgcga cacggctcat ttcctggtaa tcttcggtaa tgattaattt catcttagtg
    55621 ccttatttta tttaaataat ttctgtacga aggctgcata ggcaggccgc cagacatcca
    55681 tttcatggtt caggccggga tattcctggt aatcaaactt aatttttttc tgctcaagct
    55741 cagttttcag cccggcgata tccttgccgg ttacgacatc tttatcacca acgaccacag
    55801 taaaattacg tagttgctgg ttaatagctg ccggatcgtt cagccgggcc gcgacacctt
    55861 catccggtac ggttgtcgtg gtaacaccac tgaatgtggc cagccagcca aagctttcca
    55921 gatgattcat tccggaaacc agcgcctggt acccgccttg tgaaagccct gccagcgcgc
    55981 gaccatcggc atctttacgg acattaaaac gcttgctaat cagcgggata atatcgttca
    56041 tcagttcgcg atctgccgct ttagcattca gcggataaaa gactttacgt ctttcctgag
    56101 gcacgaaatc ttcgggaata atgcccttcg catcggtttc ggtatccggg atcaccacca
    56161 gcatcggttt aattttccct tcagccagca ggttatccat gatttgcggg atacgcccct
    56221 gatcgatagc ggaacgtccg gtatcaccaa aaccgtgata gaaatagagc actggcaaag
    56281 gctcgcccat gccggtgtaa cctggcgggg tccagacata catctgacgt tcagattgca
    56341 atgcgttgga gtggtaagtt atggcgatca aatcaccatg cgcaacagaa cgcgtatcca
    56401 gataactgcc tggcaccaga atcatactgg agttaacctg gcgctgaggc ttagtcattg
    56461 cggtgcctgt atcaatgctg cgtacaccat caacattgaa aaaatactcg tacaggttgc
    56521 ctttcaggat aggtgtgcgc cacgaccaga ctcctgcttc gtctttggtc atcgggtgaa
    56581 tattgtccgg aaccggaaca cccaccacta cagagacatt ttttgcccca ggggcaaagt
    56641 agcgaaaggt cacgctgtta tcggcattga cctgagtcac atactgtttc accgggatag
    56701 tgggtgccgg gctggctggc atatccgctg ctatggccca ctgagcagaa ataccgctgg
    56761 caatagccag cgttaaagca gcaattttta tattcatcgt aatattcctt tcattaagtt
    56821 ccattaccac cagatttcag cctgggcacc gacagcgaac tggtcgtctt tattgtcttc
    56881 gaaggtgaag ccatccagtt cgttttccag ggctttgata tacgtggcgt agaaacgaat
    56941 ttccggacgc gaggtcaaca tactggtatt gactttaaag gtatggaaga gcgtggtttt
    57001 ataaccagac tcattaaatt tattactatt cgcatctttg ttttgctggg tgaaatagcc
    57061 cagttcaaca cctgtctggt tatattggtc ccaaatatag gccgggcgaa caaccgcacg
    57121 aatagattcg aaatcagagt gtgcgcctgt ttcgtagcta taaatatcgt taccgaagga
    57181 gtaaacaatc gcgttagcca caataaaatg atcgccgata taggcttcgc cctgtgaagt
    57241 cagacgaact gctgttccgc cggtgtgatc accataataa cgaccattaa aggtggtaaa
    57301 tggacttgcg ccagcataac ggccaaagtt actggcgata gagttattcg caaccaggaa
    57361 ggagaattcg ttgaagccac ctttatcaaa tttctgcgtt aaagatgtgc caaacatcca
    57421 ggtatctttc cagtcataat agccgttatt atcctgatta tctttttccg atgcgctttc
    57481 gtttgccgtg acataacgac cactcaccat taaggtcgct ttatcccata acgggatatc
    57541 tttatagcgt aaatcaatag tattggtatt aatctgctgt ttgttttgca ggctgcgatc
    57601 gtaatcatcg acatcttcgc gaaccagcgc gatatcaatt ttacccggac cgactttcca
    57661 gttttccaga cctacacccg ctgcggcatc agtacgctgc gttttccagt ccagcatctg
    57721 gatttcaatt ttcggcgcac cgtgtttacc cacccagaaa tcagcctctg gcgcaaaggg
    57781 caggaaacct ttggtggtaa cgtacatatc ggagaactgc ataaagttct cgccaccggc
    57841 gttatcgcca aaccagccgg tagagtactg ctgaccaacg ttaccatcca tcatcacaac
    57901 ggcatcaacc cgtttgccgt tttcgttgta gacacgttgt tttaactgca aatcaaacca
    57961 gccggagtat tcgttaccaa agcggcccag agaaccaatc gcccatgatt taggtgaacc
    58021 atgagaggcg gtcccccaac cagaacggta atagccgttg taactaaagc caatttcatc
    58081 tttcacaaac ttactgaaat ctttcagagt catggaagaa taaatcgagc ctgtctcact
    58141 ggcatttttc tcttcatttt tgaccagtac agcatcaggt tttgcggcac tactggtcgg
    58201 gaacggattg gcggcatacc cttgatcatt cgtacttacg ctacgattca ccgtcgctgg
    58261 cgtatatttt ttcttctctt catctttata ctttttgagt tcgctttgcg tttctcttaa
    58321 cgctttttct aataactcaa ggcgatgttc caccgttaat gattcagcca atgattgtgc
    58381 agaaaaggct aacggtgcca ttaataagat ggcagaggta ataagatttc gtctaaacat
    58441 aatatccctt tatggtgcaa agaaagaatt aacgtatcgc atccagactg ttctgaatgc
    58501 gacgataatt aaggtgcttt attggttatt tttttaatga cagcccccgc gttttgatca
    58561 cttctgcata ccatccgaag cttttcttac gcgttcttgt gaggcttcct tcgccattat
    58621 catcacgatc cacataaata aagccgtagc gcttagacat ttgtgaatga gatgcactga
    58681 ctaaatcaat tggcccccaa ctggtgtacc ccataatatc cacaccatcg gcaatcgctt
    58741 catttacctg taccaggtga tcgtttaaat aggcaattcg ataatcgtcc tgtatcgaac
    58801 catccgcttc aacgctgtct tttgcgccta atccgttctc gacaataaat aacggttttt
    58861 gataacgatc ccaaagcgta tttaacagaa cccgtaatcc aaccgaatca atttgccacc
    58921 cccactctga acttttcaga tgcggattgg ggatcatatt cagtatgttg ccctgcgcat
    58981 ttttattaat gctttcgtcg tgggaaacac aaccagtcat gtaataacta aaagagatga
    59041 aatcgacggt atgttttaaa tcttctgcgt cactttcagt catctcaatg gtgatattgt
    59101 ggtcgcggaa gaaacgctgc atatagccgg gatactggcc acgcgcctga acatcaccaa
    59161 agaacatcca gcgccggttc tcttccatgg cctgcaacat atcctgtggc tggcaggtga
    59221 gggggtaaac cagcccaccg agaagcatat tgccgatttt cgcttcgggg agcaggctat
    59281 gacaggcttt aactgcccgc gcactggcaa ccagttgatg gtggatagcc tgataaactt
    59341 ccgcctcgcc actctcttct gccagcccca cgcccgtgaa tggctcgtgt aacgacatgt
    59401 tgatttcatt aaacgtcagc cataacgcca ctttatgttg gtagcgagta aagaccgtgc
    59461 gggcgtaatg ctcgaagtga tcgatgaccg ctcgattagc ccaaccgccg tagtttttca
    59521 ccagcccata tggcatttcg taatgggata acgttaccag cggcttgatc cccgcctgcg
    59581 ccatttcatc aaacagccga tcgtaaaacg ctaaccccgc ttcattcggt tcgacttcgt
    59641 cgccctgagg gaaaattcgc gcccaggcaa tggaaatacg cagacaggtg aagcccatct
    59701 cggcaaataa cgcgatatct tccgggtaac ggtgataaaa atcgatggcg acatctttga
    59761 tattctcttt ccccaggatg cgcggttcca tttttcccat tacgccatga ggctgtaaat
    59821 ctgaggtcga gatccctttg ccatcttcct gccaggcacc ttccacctga ttggcagctg
    59881 ttgcgccacc ccaaagaaat gtttctggaa atgctttcat aattaactcc ttttatcgtt
    59941 agcgaatgat ggataacagc ggttcacctg cgcttatctg cgccgtgccg tggggtaata
    60001 cgtccgtaaa atcatcgcta ttactgatta ataccggcgt cgtcagatca aatccggcct
    60061 cgcgaatagc agggatatca aaagaaatca gccgatcgcc tgtattgacc ttgtcaccca
    60121 cgttgacgtg agcggaaaag aatttgccgt ccagttttac ggtgtcgata ccgacatgaa
    60181 tcaggagctc cacaccatca tctgactcaa tgccaatggc gtgtaatgtg gcgaacaacg
    60241 aagcaattcg acccgcaacc ggagaacgca cttcaccaac cgagggcaga atggcgatac
    60301 ctttacccaa caggccactg gcaaacgtgg tatcagcgac gtgaatgagc ggcacaatct
    60361 ctcccgtcat cggtgaacag ataccgccct gctcaggtgg tgtaataacc tctggtgttt
    60421 tctcttgcgg ggcaccctgc gctggctgac gtttagcggt gatgaaatga agcatcaccg
    60481 taccgacaaa tgcgcaaccg atggcaatga caccgccaat aacgctggcc cagacggtga
    60541 aatcaattcc cgttgacggg atggtttgca tgaaggtgaa aatacttggc aaaccaaagg
    60601 agtacacttt cgtttgcgcg tagccaatga tggtggcccc caaagcccca ctgatacagg
    60661 cgataacaaa ggggtactta cgcggcagat taacgccata taccgctggt tcggtgatac
    60721 caaacaggcc cgtcaacgcc gctgatcccg ccaccacttt tttctgcgca tcgcgttcgc
    60781 agaggaagac gccgagcgcc gccccgacct gcgccataat ggcgggcatt aacagcggga
    60841 tcatggtgtc gtagcccagc acggtgaagt tattgataca cagcggcacc aggccccagt
    60901 gcagtccgaa catgacgaag atttgccaga agccgcccat taccgcgcct gcaaatgcag
    60961 gaaccgcctg ataaagccag agataaccgg cggcaatcag ttcgcttatc caggttgata
    61021 gcggccccac cagcagaaag gtgacgggtg tgataaccat cagacatagc aatggtgtga
    61081 agaaattttt gattgccgac ggtaaccacg cattaagtcg gcgttccaga atgctgcaca
    61141 accaggcaga aaaaataatg ggaataaccg atgacgagta attcaacaat gtgaccggaa
    61201 tacccaggaa atccagcccc agctcatccg ctttttgccc gttctcgaaa gcagtcagaa
    61261 ttaatggatg cactaacgct ccaccaatca ccatggcagt aaatggatta ccgccgaagc
    61321 gtttccccgc ggtgtatccc aggattatcg ggaagaacca aaacaaggca tcactggcgc
    61381 tgaataaaat taaataagta ccactttgtt cggtcgtcca ctgaaaagtg agcgccagag
    61441 ccagcatacc tttcaggatc ccggttgccg ccatcaaacc gatcagaggc gtaaaaatac
    61501 ctgaaataac ataaacaaag cggtttagca gattaccttt ctcatcattt tccggtgcct
    61561 gttgcgcttt ttcgccaagg cctgccacgc tgttgaccgc caggaagaca tcggccacat
    61621 ggttacctat gaccacctga aactgaccac cgctttccac caccataata ataccggggg
    61681 tctttttcag tacctcagct tgcgctttgc tttcatcctt taacttaaaa cgtaatcgcg
    61741 ttgcgcaatg catcagactc acaatgttat ctgcgccccc gactcctgcg actatttttc
    61801 tggctaactc cgtcataact tgccctctac cgctttgcgg caaaactcca aaaaaaaacc
    61861 tgaaaaaaac ggcctgacgt gaatcaagca atttttttca ggttttgccc gcttagtgcg
    61921 gtaacaatcc tttactcagt aataatattt cagtgttctt tgcgcacgcg ctctatattt
    61981 atggctaaaa acataatctc tgcgggtgaa attttacgtt gatactgcaa accaataaaa
    62041 atggcgatcc gttccgcaca ttgccatgct tgcgggtaat tttgttttac tgcttgttgt
    62101 aatgattcat cactatcgtt aattgaagca tgttcaagaa tacgccagga taaaaacttc
    62161 agatgtgtaa ccagtcgctg ataactcaag ctttcttcct ggtaattaag gctgaactga
    62221 aattttatta attgcagcat ttcgcgcatt aactgcgtga cacctgcaac atcctccata
    62281 tttccgctca tttgggcact gaccagatgc atggcaataa agcccacttc atctttcggt
    62341 aactgcacgc ccaaccgttt atcaataatg gttaacgctt cttcccctag ctggaactct
    62401 ttcgggtaaa gccgctggat atcccacagc aacgggttgg gcagcaacac gttttgctga
    62461 aagcgtttaa tcgcaaactg gcaatggtca gttagcgaga tataaatact gtcctgtaat
    62521 tttcccaagc gctcctgcgc taaagagata atacgatcac aggttgccat cacctcaaga
    62581 ggaatatgac ttaagagttc gcttaatcgc ccgttcagtt catgactgct caaggcatac
    62641 tctttttcta ttccacttga gttaattctt tcgccagcgc gtttttgaaa gccaattcct
    62701 cgccccatga cgactttttc ccgctgttga tcatcaataa ccaccacaac attattgttg
    62761 agaattttgg tgatttgcat gttcatagca aggacctttt ttataaacaa aaaaaaccga
    62821 cttcaccagt attctctggt tatgtcaggt tttgcctgcg aatgcagtaa caatccagtc
    62881 atttattaat ggtttttata acgaacatcc aggttcggaa attaatttaa ttgcgtgcag
    62941 agaaaatagc aatgcgctat tgataaaaat atgaccatgc tcgcagttat taactttgtg
    63001 taattttagg aatttataaa gttatatata acaaatccca ataattaagt tattgggatt
    63061 tgtctggtga attatttgtc gctatctttt cccgccagca gtttatccag ctcatcaccg
    63121 cctacatgac ggaaatcctg ccccttcacg tagtagaaga taaactcaca aatattctgg
    63181 cagcggtcgc caatacgttc gatagaacgc gcgcagaaca gcgcagtaag tacgctcgga
    63241 atggtacgcg aatcttccat catgtaggtc atcagttgac gaacaatacc ttcgtattcc
    63301 tgatcgactt ttttatcttc acgataaata cgtaccgctt cgtcaatgtc catccgcgcg
    63361 aacgcgtcca gcacgtcgtg cagcatctgg atggtatgac ggcccagcga ctccagactt
    63421 accagcaacg gctgatgctg ctgggagaat ttctccagcg cagtacggca gattttgtcc
    63481 gccacgtcgc caatacgctc cagctcggca atggttttac tgatcaccat aaccagtcgc
    63541 aggtcgctcg ccgtcggctg acgtttggcg ataatgcgca cgcaggcttc atcgatcgcc
    63601 acttccatca tgttgacgtt cttgtcgcct tcgatcacgc gcttcgccag atcgctgtcc
    63661 tggttatgca tcgcggtgat tgcatcagaa agctgctgct ccaccatgcc gcccatggtc
    63721 atcacctgcg tgcggatact ttccagttcg gcgttgaact ggccggaaat atgtttatta
    63781 agattgagac tgtccataac gcactcctga atcaaccgta acgaccggtg atgtagtctt
    63841 ctgtttgttt cttcgctggc ttggtgaaca gatcgtccgt gttgctgaac tcaatcaatt
    63901 cgcccaggta cataaacgcc gtgtggtcgg aacaacgcgc agcctgctgc atgttgtggg
    63961 tgacgatcac cacggtgtaa tcctgcttca gttcggtgat cagctcttca atacgcccgg
    64021 tagagatagg gtcgagcgcc gaacacggtt cgtcgagcag cagcacttcc gggcgaatgg
    64081 cgataccacg cgcaatacac agacgctgtt gctgaccacc agagagagag taaccgctct
    64141 ggtgcaattt atctttggtt tcgttccaca atgcggcttt ggtcaatgcc cactgcacgc
    64201 gctcgtccat gtcggcacgg gagagcttct caaacagacg aacgccaaaa gcgatgttgt
    64261 cgtagatgga catcggaaac ggcgtcggtt tctggaacac catgcccact ttcgcacgca
    64321 gcagggcgat atcctgagag ttggtcagga tgttgtcgcc atcaagcaga atttcacctt
    64381 ccgcacgctg ctccgggtac agttcaaaca ttttgttgaa ggtacgcagc agcgtcgatt
    64441 taccgcagcc ggacggcccg ataaacgccg ttacctggtt tttagcgata tccaggttga
    64501 tgtttttcag ggcatggaat ttgccgtagt agaagttcaa attacgaacc tgaattttac
    64561 tcggggcagt ttcaaccata ctcattgcaa tcttttcctc atcgtgcctg atgcacttcg
    64621 ctaatcaggc ttaccgtagg ccggatcagg cgttcacgcc gcatccggca aagtgttaac
    64681 cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagt acgcacaggg
    64741 taatgatcaa taccccggcc caggccagtt gctgccattc cgcaaacgga ctcatcgcaa
    64801 acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg ctccagaact
    64861 ggttggagag cgcggtaaac agcagcggcg cggtttcacc agcaatacgg gcaatcgcca
    64921 gcaagatacc ggtcatgatc ccggacaccg acgctttcag cgtaatcgcg gagatcatct
    64981 tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc accagtttca
    65041 gcatgttctc ggtggtgcgg ataacaatcg gcacctgcaa cagcgccagc gcaattacgc
    65101 ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca aacagaccaa
    65161 ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact tctgccagcc
    65221 aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc ggcgtaccga
    65281 ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc agaccaccac
    65341 cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata ccgtcgatac
    65401 cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag gccatcgtcg
    65461 ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc attttgcggc
    65521 gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc gtgccccctc
    65581 attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga aggtaatcac
    65641 aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt ccgcttccgc
    65701 aaattcgttc gccagcgcag aggtgatact gttgcccggc atatacagcg aggcgctgtc
    65761 gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga gcgcgcggcc
    65821 cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa cgatacgcca
    65881 gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca tcaccggggt
    65941 ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga tggcgaggat
    66001 cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg cgccaacaat
    66061 cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga acagcggcgc
    66121 aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca gcagctcaat
    66181 ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga acagggcgat
    66241 accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca acgtaccgta
    66301 gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg tccataggaa
    66361 agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga caataatgcc
    66421 acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga aaattatgtc
    66481 gcccttttta cccggtgggt taaaagcagg cttggttgca gccataagtt actcttcagt
    66541 taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac cgtacccggc
    66601 ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt cttccacgca
    66661 gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc gttcgcctgt
    66721 ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cttctgtgcc ttgttctggt
    66781 ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca tgcatcttcg
    66841 cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc ttttgctgca
    66901 ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat cagtttggtg
    66961 tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc acccggcaga
    67021 cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat cggccatttt
    67081 acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc caggtagctg
    67141 gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat gttttgtgaa
    67201 ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt cttgattttg
    67261 cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga cttcagccct
    67321 ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa cagaccttcc
    67381 tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac ggtattagcg
    67441 ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt attaccggtt
    67501 tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt tgcacctgca
    67561 cctgtcaggc ttgcttctgc aaacacagag aaagcgctca tcgataaggt cgcggcgaca
    67621 acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat tcataaagca
    67681 ttgtttgttg gctacgagaa gcaaaatagg
//
LOCUS       DAZFJF010000011        62905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFJF010000011 DAZFJF010000000
VERSION     DAZFJF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.011"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHKAU_000835"
     CDS             complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHKAU_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO5529951.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHKAU_000836"
     CDS             complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHKAU_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO5529952.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHKAU_000837"
     CDS             complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHKAU_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO5529953.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHKAU_000838"
     CDS             complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHKAU_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO5529954.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHKAU_000839"
     CDS             complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHKAU_000839"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO5529955.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHKAU_000840"
     CDS             complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHKAU_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO5529956.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHKAU_000841"
     CDS             complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHKAU_000841"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO5529957.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHKAU_000842"
     CDS             complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHKAU_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO5529958.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHKAU_000843"
     CDS             complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHKAU_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO5529959.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHKAU_000844"
     CDS             complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHKAU_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO5529960.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHKAU_000845"
     CDS             complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHKAU_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO5529961.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13704..13781)
                     /locus_tag="ACHKAU_000846"
     tRNA            complement(13704..13781)
                     /locus_tag="ACHKAU_000846"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13744..13746),aa:Met,seq:cat)
     gene            14129..15472
                     /gene="argG"
                     /locus_tag="ACHKAU_000847"
     CDS             14129..15472
                     /gene="argG"
                     /locus_tag="ACHKAU_000847"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO5529962.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHKAU_000848"
     CDS             complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHKAU_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5529963.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17564..17650)
                     /locus_tag="ACHKAU_000849"
     tRNA            complement(17564..17650)
                     /locus_tag="ACHKAU_000849"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17614..17616),aa:Leu,seq:gag)
     gene            complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHKAU_000850"
     CDS             complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHKAU_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO5529964.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHKAU_000851"
     CDS             complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHKAU_000851"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO5529965.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHKAU_000852"
     CDS             complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHKAU_000852"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO5529966.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHKAU_000853"
     CDS             complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHKAU_000853"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO5529967.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHKAU_000854"
     CDS             complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHKAU_000854"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO5529968.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHKAU_000855"
     CDS             23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHKAU_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO5529969.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHKAU_000856"
     CDS             complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHKAU_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO5529970.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24455..25888
                     /gene="dacB"
                     /locus_tag="ACHKAU_000857"
     CDS             24455..25888
                     /gene="dacB"
                     /locus_tag="ACHKAU_000857"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5529971.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHKAU_000858"
     CDS             complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHKAU_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO5529972.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHKAU_000859"
     CDS             complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHKAU_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5529973.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHKAU_000860"
     CDS             complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHKAU_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO5529974.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHKAU_000861"
     CDS             complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHKAU_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO5529975.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29056..30027
                     /gene="ispB"
                     /locus_tag="ACHKAU_000862"
     CDS             29056..30027
                     /gene="ispB"
                     /locus_tag="ACHKAU_000862"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO5529976.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHKAU_000863"
     CDS             30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHKAU_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO5529977.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHKAU_000864"
     CDS             complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHKAU_000864"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO5529978.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHKAU_000865"
     CDS             complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHKAU_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO5529979.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHKAU_000866"
     CDS             complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHKAU_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO5529980.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHKAU_000867"
     CDS             complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHKAU_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO5529981.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHKAU_000868"
     CDS             complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHKAU_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO5529982.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHKAU_000869"
     CDS             complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHKAU_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO5529983.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHKAU_000870"
     CDS             complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHKAU_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO5529984.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHKAU_000871"
     CDS             35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHKAU_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO5529985.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHKAU_000872"
     CDS             36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHKAU_000872"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO5529986.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHKAU_000873"
     CDS             37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHKAU_000873"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO5529987.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38181..38756
                     /gene="lptC"
                     /locus_tag="ACHKAU_000874"
     CDS             38181..38756
                     /gene="lptC"
                     /locus_tag="ACHKAU_000874"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO5529988.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38725..39282
                     /gene="lptA"
                     /locus_tag="ACHKAU_000875"
     CDS             38725..39282
                     /gene="lptA"
                     /locus_tag="ACHKAU_000875"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO5529989.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39289..40014
                     /gene="lptB"
                     /locus_tag="ACHKAU_000876"
     CDS             39289..40014
                     /gene="lptB"
                     /locus_tag="ACHKAU_000876"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO5529990.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHKAU_000877"
     CDS             40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHKAU_000877"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO5529991.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41518..41805
                     /gene="hpf"
                     /locus_tag="ACHKAU_000878"
     CDS             41518..41805
                     /gene="hpf"
                     /locus_tag="ACHKAU_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO5529992.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHKAU_000879"
     CDS             41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHKAU_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO5529993.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHKAU_000880"
     CDS             42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHKAU_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO5529994.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43311..43583
                     /gene="npr"
                     /locus_tag="ACHKAU_000881"
     CDS             43311..43583
                     /gene="npr"
                     /locus_tag="ACHKAU_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO5529995.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHKAU_000882"
     CDS             43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHKAU_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO5529996.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHKAU_000883"
     CDS             complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHKAU_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO5529997.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHKAU_000884"
     CDS             complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHKAU_000884"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO5529998.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHKAU_000885"
     CDS             complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHKAU_000885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO5529999.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHKAU_000886"
     CDS             complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHKAU_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO5530000.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50070..54530
                     /gene="gltB"
                     /locus_tag="ACHKAU_000887"
     CDS             50070..54530
                     /gene="gltB"
                     /locus_tag="ACHKAU_000887"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO5530001.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54543..55961
                     /gene="gltD"
                     /locus_tag="ACHKAU_000888"
     CDS             54543..55961
                     /gene="gltD"
                     /locus_tag="ACHKAU_000888"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO5530002.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHKAU_000889"
                     /pseudo
     CDS             56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHKAU_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHKAU_000890"
     CDS             complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHKAU_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO5530003.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHKAU_000891"
     CDS             complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHKAU_000891"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO5530004.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHKAU_000892"
     CDS             complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHKAU_000892"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO5530005.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHKAU_000893"
     CDS             complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHKAU_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO5530006.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHKAU_000894"
     CDS             complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHKAU_000894"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO5530007.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHKAU_000895"
     CDS             complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHKAU_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO5530008.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        15399 a        17093 c        15526 g        14887 t
ORIGIN      
        1 ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat aaggcaccgc
       61 tgattactga tacaccggca gtaaattaaa gctcgataaa atatggacca gtgcgttgcc
      121 aacgccaaac accagaatca gcgtaatcat cggcttgccg ccccagacgc ggaatttcgg
      181 gctgccaaag cgtttacgcg atgcgcgggc taacagcgcc ggaacaattg ccgcccagat
      241 ggtagctgct aaaccagcat aaccaatggc gtacaggaat ccgttcggga acagtagacc
      301 acccacaaca ggcggggcaa aggtcagcaa tgccgttttc aagcgcccca gggctgagtc
      361 atcgaaacca aacagatctg ccagatagtc aaacaaaccc agcgttacgc caaggaacga
      421 actcgctacc gcaaagtttg agaacacgac cagcagaagg tccaggctac ggctgttcag
      481 cacgccgctt aatgcctgta ccagcacatc aatattaccg cccttctctg caataccgat
      541 aaactccgga cgcgggatgt tacccatcgt cgccagcaac cagatggtat acagcgccag
      601 tgccatcagc gtaccgtaca ccaggcattt cacgatggtt ttcggatctt tgccgtaata
      661 cttcatcagg cttggcacgt taccgtgata accaaacgac gccaggcaga acggcaatgt
      721 catcagcaga tacggcgcat aagatgcatt gctttctgca acgttgaaca atgtcgcagg
      781 ctgcacatgc cccaacaggc tgccaaaggt gaggaagaag gtaatgactt tcgcccccag
      841 cacaatcgcc gtcatgcggc tgacggcctt agtactcaac cacaccacaa acgctaccag
      901 cagtgcaaaa ccaaaacccg ccgcccgtgc cgggacgttt agcgacatct ctgcgaaggt
      961 gtgatgcaga attgaaccac tggcagaaat ataggcatag gtcaggatat agagcacaaa
     1021 ggcaatggaa ataccgttga ccacgttcca gcctttgccc agcaaatctt tggtgatggt
     1081 gtcaaaactc gaaccgattc ggtaattcag gttagcttcc agaatcatca agccggaatg
     1141 cagcatacag aaccaggtaa agatcagcgc cgccattgac cagaaaaacc acgccccgga
     1201 catgaccact ggcagagaaa acatccctgc gccaataatg gtgccgccga taatcaccac
     1261 gccgccaagc agcgacggtg acgtttgggt ggtggttagt gttgccatga gggcttctct
     1321 ccagtgaaaa atagtgcgac tgcgttgtta tgcattgcac tgtaccagta cacgagtaca
     1381 aaagacagaa aaaaagcccc gatggtaaaa atcggggctg tatatattat tttacagatt
     1441 gtgttcgctg ttcagcgatg attacgcatc accaccgaaa cgacgacgac cggtagagtc
     1501 atcacgacgc ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac catcaccacg
     1561 gccaccttca cggcgttcac cgctgaagtt acgaccgcct tcacgacgtt cgccaccgaa
     1621 accacgacca ccgccacgac gctcaccgcc agtatgcggc tgcgcatcgc ccagcaactg
     1681 catattcatc ggcttgttca gaatgcgagt acgcgtaaag tgttgcagta cttcacccgg
     1741 catacctttc ggcagttcga tagtggagtg agaagcaaac agcttaatgt taccgatgta
     1801 acggctgctg atgtcgcctt cgttagcaat cgcgccaacg atatgacgaa cttcaacacc
     1861 atcatcgcgg cccacttcaa tgcggtaaag ctgcatgtca ccaacatcac gacgttcacg
     1921 acgcggacga tcttcacggt cgccacgtgg gccacggtcg ttacgatcgc gcggaccacg
     1981 gtcatcacgg tcacggaatt cacgtttcgg acgcatcggc gcatctggcg gaacaatcag
     2041 agtacgttca ccctgtgcca ttttcagcag tgccgcagcc agagtttcga gatcctgctc
     2101 ttcaccttct gcagtcggct gaattttgct cagcagtgcg cggtattgat ccagatcgct
     2161 gctttccagc tgctgctgta ctttagcggc gaatttttcc agacggcgtt tgcccagcag
     2221 ttctgcgttc ggcagttcta cttccggaat agtcagcttc atagtacgtt caatgttgcg
     2281 cagcagacga cgctcgcggt tctcaacgaa cagcagcgcg cggccagcac gacccgcacg
     2341 accggtacga ccgatacggt gaacgtaaga ctcagaatcc atcgggatat cgtagttaac
     2401 taccaggctg atacgctcaa cgtccaggcc acgggctgca acgtcggtcg caatcaggat
     2461 gtccagacga ccatctttca ggcgttccag tgtctgttca cgcagcgcct ggttcatgtc
     2521 accgttcagc gcggcgctgt tgtagccgtt acgctcaaga gcttcagcca cttccagagt
     2581 cgcgtttttg gtacgaacga agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg
     2641 taccagtgct tcgtttttgc gcatacccca gacagtccag tagctctggc tgatgtcagg
     2701 acgggtagtc acgctggact gaatgcgcac ttcctgcggc tctttcataa agcggcgggt
     2761 aatgcgacga atcgcttccg gcatggttgc agagaacaga gcggtctgat gaccttccgg
     2821 gatctgcgcc ataatggttt caacgtcttc gatgaagccc atgcgcagca tttcgtcagc
     2881 ttcatccaga accagaccgc tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc
     2941 cagcagacgg cccggagtac cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg
     3001 cacgtcataa cgctggccgc cgtatagagc aaccacattt acgccgcgca tgtgtttaga
     3061 gaaatccgtc attgcttcag caacctgtac cgccagttcg cgggtcggtg ccagcaccag
     3121 aatctgtggt gctttcagct caggatcaag attctgcaac agaggtaaag agaatgctgc
     3181 agtttttccg ctccccgtct gggccatacc cagaacgtcg cggccattca gcagatgtgg
     3241 aatacactct gcctgaattg gagatggttt ttcgtaaccc agatcgttaa gggcttcaag
     3301 gataggagcc ttcaggccca gatctgcaaa agtggtttcg aattcagcca tgtagtacgt
     3361 gtgcctcaaa attaatggcg gccagtctac ataactcatc atgaaattga tcagcaattt
     3421 tcattgaaaa gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc
     3481 cgttaaggtg atggcaatca aaaaagatta cgggctgatg tgtacgtcag ctattgctgg
     3541 tccgattctg ccaggtcatc ttggtcctgg cccaggagcg ataattccaa caatgcgtat
     3601 cggtgctcaa caaagttatg aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg
     3661 ctgtccaaat cccccagact taggtagtac ttacctaaat agaagttggt ttcactgaga
     3721 tgctcagcga gcgaggtgtt atccgttgcg tccgccttga gcctttccat taacgtttgt
     3781 tcgctaatgt tgcccaggta gaactcgaca atgttccatc cccactgttc cttatccgat
     3841 ttttcgaagt gctgtttcaa cacttcttta gcctgcttct catcgagctt ctgctcggcg
     3901 agataaagcc acagactacg gaaaggatca ttgggatcgt cttgataaaa cgccagcaga
     3961 tcatcttgcg ctaacttgtc acgaccgccg taatataatg cgatcccgcg attcaagtgc
     4021 gcgtagttgt aagttggatc aagctcaagt acagaatcaa acgcttcata ggcagcatca
     4081 aaattgcctg cctgcgttaa atatatgcct aagtaattga atacttcagg catatccggt
     4141 cggattgcca gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc gagactatca
     4201 tacaacactc cgcgctcata taaaagctgt gcgcgttcgt catcggttaa agcccgactg
     4261 gcaaggattt gttccatacg tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt
     4321 accgcgagga cttcactttt acgccaggaa gtattactgc atcctgcaag cgtaagtgct
     4381 gtcgcaacga aacaccagcg caaaaaaggc ttcatttccc actcccgaag accacggttg
     4441 aatgaacgtc ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc
     4501 gcagcggagg gcaaatggca accttactcg ccctgttcag cagccggagc ttccggtgct
     4561 gcagcaggtt gagactgctc agtcgcttct ttaatgctca gacggatacg gccctggcga
     4621 tcaacttcca gaactttcac cggtacttcc tgacccatct gcaggtaatc ggtcactttc
     4681 tcaacgcgtt tgtcagcgat ttgagagatg tggaccagac cttctttacc gccgccgatg
     4741 gcaacaaatg cgccaaagtc aacgatacgg gtcactttac cagtgtagac gcggcccact
     4801 tcgatttctg cagtgatctc ttcgatacga cgaatagcat gtttcgcttt ctcgccgtcg
     4861 gtcgctgcga tcttcacagt accgtcatct tcgatttcga tggtagtgcc agtttcttcg
     4921 gtcagggcac ggataacaga gccgccttta ccgataacgt ctttgatctt gtccgggttg
     4981 atcttgatgg tatggatacg cggtgcgaac tcagagatat cgccacgcgg cgcgttgatc
     5041 gcctgttcca ttacgcccag gatatgcaga cgcgcacctt tagcctggtt cagcgcaacc
     5101 tgcatgatct ctttggtgat accttcaatt ttgatatcca tctgcaatgc agagataccc
     5161 tcgcgggaac ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg
     5221 tcagacagta caacgtagtt gtcgccttct ttcaccagac ccattgcgat acccgcaacg
     5281 gcagctttga tcggcacacc tgcgtccatc agcgccagag acgcgccgca cacggaagcc
     5341 atagaagagg aaccgttgga ttcagtgatt tcagacacaa cacgtacggt gtacgggaat
     5401 ttgtccatat ccggcatgac tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt
     5461 tcacgacgct tcggagaacc gaccatgccg gtttcgccta cggagtacgg agggaagttg
     5521 tagtggaaca ggaaggtatc ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca
     5581 cgagcagtac ccagcgttgc ggtaaccagc gcctgcgttt caccacgggt gaacagcgca
     5641 gaaccgtgag tacgcggcag cacgccagta cgcacatcca gaccacggat catatctttt
     5701 tcacgaccgt cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac gtttttctcg
     5761 atagcgtgca gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc agcaagcagc
     5821 gtcgcgatgg tttcagattt gatgacatca acctgcgcat aacgctcttg tttgtcggtg
     5881 atgcggtaag catcgctcag gcgagcttca gccagtgcag caacgcgcgc gttcagcgct
     5941 tcgtttaccg gctccggctg ccagtcccaa cgtggtttac cggcttcttt caccagttca
     6001 ttgatgttct gaataacaac ctgctgttgt tcatgaccga acactactgc gcccagcatc
     6061 tggtcttcgc tcagcagttc agcttcagat tcaaccatca gaacagcggc ttcagtaccg
     6121 gcaacaacca gatccagttt gctctctttc agctcgtcct gagtcgggtt cagtacgtac
     6181 tggtcattga tgtaacctac gcgagcagca ccaatcggac cattgaacgg aataccagac
     6241 agagacagtg ctgcggaagc accaatcatc gcgacgatat ccgggttaac ttgcgggtta
     6301 acagaaacca cggtggcgat aacctgaact tcgttgacga agccttccgg gaacagcggg
     6361 cgaatcgggc ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg gcggccttca
     6421 cgacggaaga agctacccgg gatacgacca gcagcgtagg tacgctcctg atagttaacg
     6481 gtcagtggga agaagtcctg acctggtttg gcttttttct ggccaacaac ggtaacaaat
     6541 accgcggtgt catccatgct aaccataaca gcggcagtag cctgacgagc catcatgccg
     6601 gtttccagag tcacggtgtg ttggccgtac tggaatttac gaacgatcgg attaagcaat
     6661 gtaatatcct ttctctttct tagacagtac cttacggcac tggtgttaat acccgatctt
     6721 ctgcgcatcc tcgcgactaa tgacaaccct aacccagctc tatgtgggta aagcctctca
     6781 ttagccgcgc gaacctctgc aacggaagat cattcatagc aacaatacat tagtttccag
     6841 tgaattgctg ccgtcagctt gaaaaaaggg gccactcagg cccccttttc tgaaactcgc
     6901 aagaattagc gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta
     6961 cgtttcaggt agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg
     7021 ctgtggtgat cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc
     7081 agcagtgcta cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa
     7141 acgattttag ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat
     7201 gaaaggacgc cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa
     7261 tttacgcgac gttaagcggc agtattctac tcgtagcgac ctgttatcgc aagacggtta
     7321 acattacgcc gggtattcaa ccaccaggcg acgaggcgca acgcggcctt catcgtcaat
     7381 ttcgcccata ccgataaatt tgccgttctc accttctgta acgcgaacca gtccttccag
     7441 cggcgcaccg gatgtacgaa ctgggttacc atttttgaag taaacagaag acgttaacgg
     7501 aagattcacc accgggtagt ccgaagctgg actgtccatt ggcatcagta atggatcaag
     7561 taactccgca gccggaatat cctgctgttc ggcttgttcg accagttcac gcagatgttc
     7621 cagggtcacc atccgttcaa ccggatattt acttaccgcc agacgacgca ggtaaataac
     7681 atgcgcgcca caaccgagtt tttcacccag gtcatcaatg atggtgcgga tataagtgcc
     7741 ttttgagcag tgaatttcca gctccagctc attgccttcg tggcgaataa acagcaattc
     7801 ataaacggta atcgggcgcg cttcacgcgg tacttcaatg ccctgacgcg catattcgta
     7861 cagtttttta ccctgatatt tcagtgctga atacatcgaa gggatctgtt cgatatcgcc
     7921 acggaaagta tccagcgccg ccgccagttg ctctgcgcta aaggttaccg gacgttcttc
     7981 aacgatttgt ccgtcggcat cagaagtatc ggtacgctgt ccaagacgcg caatgacccg
     8041 atagcgtttg tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat
     8101 tggcaacatg ccagttgcca gcgggtccag cgcaccggta tgcccggcac ggttggcgtt
     8161 ataaatccgt ttcacttttt gcagcgcatc attgctggac ataccctgcg atttatccag
     8221 caacaaaacg ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg
     8281 ctgtcgtccg ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt
     8341 gacatgcgca tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata
     8401 cgcaggcgca tcgctttccc cagcaggctg cggatgaaac cagaagcttc ttgcaacgct
     8461 ttgatgcccg ctttaaccgc gtcttcatct ttgtcgttga ggaacgtcac atatactttg
     8521 gcatacgcca ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccaggcga
     8581 ggatctttaa tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg
     8641 cgctgcgggc gaccaaattc tttcgccata ataaattctc ctgacaaaaa aggggctgtt
     8701 agcccctttt taaaattaat ttcaggtgga agggctgttc acgttggcct gataagacgc
     8761 gccagcgtca catcaggcaa tccatgccgg atgcggcata aacgccttat cccgcatgga
     8821 accctaaaaa ccttaagcga tggtacgttg gatctcgatg atttcgaata cttcgatcac
     8881 atcgccagtg cggacgtcgt tgtagttctt aacgccgata ccacattcca taccgttacg
     8941 gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt cgtagataac
     9001 cacgttgtca cgcagaacgc ggatcgggtt gtgacgttta accacacctt cggtaaccat
     9061 acagcctgcg atggcaccaa atttcggcga tttgaacacg tcacgaactt ccgccagacc
     9121 gataatctgc tgtttcagtt ccggagacag cataccgctc atcgccgctt tcacttcgtc
     9181 aatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt caatcacttt
     9241 acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcgttgg acgccgcagc
     9301 cagggtggcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct tcactttaac
     9361 ttcgtcagta gacagtttca gcaaggagtc ggagatcgct tccacagaac cctgtacgtc
     9421 tgccttcagg acgatgttca cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt
     9481 ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac cctgacgata
     9541 cagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat cacccgcagc
     9601 cggtacgccg gacaggccga ggatttccac cggaatggac ggacccgctt ccagcacttc
     9661 ctgacccagt tcgttacgca tcgcacgaac acgaccgtat tcgaagccac acagaacgat
     9721 atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac cacgaccttt
     9781 atcgaggaag gattcgataa ccgcaccgct cgccatacct ttacgtaccg ctttcagctc
     9841 cagaacttcc gcctgcagca ggatagcgtc cagcagttca tcgatgccgg tacccgcttt
     9901 cgcagatacg tgtacgaact ggctttcacc gccccactct tccggcagga tgccatactg
     9961 ggagagttcg ttcttaacac gatccggatc ggcttctggt ttatcaatct tgttcactgc
    10021 aaccaccacc ggtacctgcg ccgctttcgc gtgctggatt gcttcgatgg tctgcggcat
    10081 cacgccgtcg tcggcagcaa caaccaggac tacgatgtcc gttgcctgcg caccacgtgc
    10141 acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca tgccgttttc
    10201 agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc cagaggcaac
    10261 tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt gacccatgat
    10321 ggtcacaacc ggcgcgcgcg gttcagccgc agcacccgtg tcacggtcgc tcattaccgc
    10381 ctcttccagc tcgttttcac gacgcaggat aactttgtgg cccatctctt cagcaaccag
    10441 ctgtgcggtt tcctgatcga taacctggtt gatggttgcc attgcgccca gtttcatcat
    10501 cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc caacggtgat
    10561 ggtttcgccg atcacaacgt cacggttaac ggcctgagct ggcttctgga agccttgctg
    10621 cagggaagaa cctttacgtt ttccgccttt accgccacgt actgctgcgc gtgcttcttc
    10681 acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg ctttcgcgtt
    10741 acgaccacgg ccacgaccgc cttcgacttc acgatcgctt tcgtcttctg cctggcgagc
    10801 atgttgagaa gtagtgacgt gataatcgct ggaatcttca gtcggttccg cgttatcagt
    10861 ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc
    10921 gagtttacga cgcgcttctt cttcagcttt acgcttgagc tctgcagctt cctgctcacg
    10981 gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattgc tcactttgtc
    11041 tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt gttctgcggc
    11101 ctcacgttca gctttttgtt gcgcctcgcg tttagccgat tcttctgcct cacgacgggc
    11161 ttgctcttcc gcttcacgct gcgcttgctc ttccgctgca aggcgttcag cctcttgcgg
    11221 atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt tgctttttcc
    11281 accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca atttgtccgg
    11341 gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg cagacacaga
    11401 gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc gttccacgga
    11461 ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt tccttcctgc
    11521 tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca taatcagtgc
    11581 tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc cctgttcggc
    11641 gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat cacgatctat
    11701 cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt cttcctgggc
    11761 ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa cggtcggctc
    11821 atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt cttccagcgt
    11881 cgagaagcct tcttctacca gaacagtcgc gaagtcttcg tcgatgtcga gatatttggt
    11941 gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt cgtcaacggt
    12001 catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct gaccgttacg
    12061 gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt gtttatcttc
    12121 atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacaa actgcgccgg
    12181 gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag aaaccgcctg
    12241 aacacgcgcg ccacgcatac ctacacaggc acctaccgga tcgatacgtt tatcgttggt
    12301 tttcaccgcg attttcgcac gagaacccgg atcgcgagcc gctgctttaa tttcaatcac
    12361 ttcttcgccg atttctggca cttcaatacg gaacagttcg atcagcattt ccggcttgga
    12421 acgagtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataga gcacgccacg
    12481 aacgcggtcg ccagggcgga agttttcacg cggcagcata tcttcgcgca ggatcacggc
    12541 ttcagcattg ttgcccagat ccagagagat gttgtcgcgg tttacttttt tcaccacgcc
    12601 ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac gttcggcttc
    12661 acgcactttc tgcacgataa cctgttttgc cgtctgggta gtgatacggt caaaggtaac
    12721 agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt cataacgtgc
    12781 ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta accagcgacg
    12841 gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg cggacgtcga tctcttgttc
    12901 atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg
    12961 aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt tgttcatcgc
    13021 gggcttttca cctcatccag actattaaaa gtggggaacc aggttcgcct tctggatatt
    13081 actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt caccgtctac
    13141 cgctttgata acgccctgcc atttacgacg gttttgtacc gccatacgga gaaccagagt
    13201 cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc gatcgagacc
    13261 cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat ccagcacagc
    13321 acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt cactatcaat
    13381 atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca gttcaaaacc
    13441 cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg tggacaagcc
    13501 cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca gataacaaaa
    13561 aaccccgata aatcggggct ttatataact gaaccctata accgcaactg cggtctggag
    13621 cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc acagtatatt
    13681 tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga cgaccttcgg
    13741 gttatgagcc cgacgagcta ccaggctgct tccaccccgc gcctgaaacg tggcaaattc
    13801 tactcgtttt gggtaaaaga tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    13861 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaataac
    13921 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctctccc
    13981 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    14041 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    14101 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    14161 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    14221 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    14281 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    14341 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    14401 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    14461 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    14521 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    14581 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    14641 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    14701 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    14761 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    14821 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg
    14881 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    14941 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    15001 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    15061 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    15121 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    15181 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    15241 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    15301 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    15361 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tctcctcctc
    15421 tgccacttca ggcgtgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    15481 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    15541 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    15601 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    15661 ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacgcca
    15721 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    15781 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    15841 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    15901 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    15961 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    16021 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    16081 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    16141 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    16201 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    16261 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    16321 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    16381 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    16441 atacctgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    16501 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    16561 gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    16621 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    16681 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    16741 aaaaaagcaa aggcacatag ataagggata tacatcccca gcattttgac aacttcatcc
    16801 ggatcactct ggagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    16861 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    16921 agcaggcgaa gcggaaggga tgatatacgt tttacaacca ggatggtcag gacgaagaac
    16981 agcaatcgct gaaccatatg cgcgccggaa gaggcgacta agttcgtaat accaaaaaca
    17041 ataacacaaa gatacaacaa ccaatgagat ttaaaacttc tagcaaattt gttgaatact
    17101 gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta
    17161 tatcagacat gtttttcaac tctgatccat atcagaccga aacactgtta gttttttctt
    17221 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    17281 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    17341 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    17401 tcagcagaag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag
    17461 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
    17521 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    17581 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    17641 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    17701 agctggttga gtttgttcgg ttttcaccgg tgcactcaga ttttcccatt cgctaccttt
    17761 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    17821 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    17881 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    17941 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    18001 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    18061 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    18121 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgctgaga
    18181 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
    18241 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    18301 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
    18361 gcagcttcaa cctctgcggt cacggcttta actgactcat gctcaagtgg atcgccgcta
    18421 cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    18481 tcgtgcaggc tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg
    18541 ccgtcaccgg tagtagtttt atccagtagg atcacatgac cggaattctc tgcaccgata
    18601 cgccagcctt tctcctgcat tttttccagt acgtagcggt cgcccacttt cgcgcgcgca
    18661 aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    18721 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    18781 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccatcgccg
    18841 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
    18901 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
    18961 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    19021 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    19081 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
    19141 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
    19201 agtttggtgc cgtcgataga gaagaattta atgccattat cgtagaacgg gttatgagat
    19261 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    19321 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
    19381 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    19441 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
    19501 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    19561 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    19621 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
    19681 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
    19741 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    19801 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    19861 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    19921 cacttctgca aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg
    19981 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    20041 ggaaagggag cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac
    20101 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    20161 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
    20221 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg atcatcagat tcgcatgttt
    20281 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
    20341 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
    20401 catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
    20461 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    20521 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
    20581 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    20641 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    20701 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
    20761 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    20821 tgtttcgctt tcgctacgct acggccgagg aatacttcac cttcttcttc cgcgtacagc
    20881 agtggaccca atttctcaga gaagccccac tgggtcacca tgttacgtgc cagattggtc
    20941 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccata gatgatctct
    21001 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
    21061 ctgattgcgt cgccttcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    21121 atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc
    21181 gcttcgtggt aagccgttga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    21241 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    21301 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    21361 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggggccaa tggtacgcga
    21421 cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc
    21481 tgacggtcga aacggccagg acgcagcagg gccgggtcga gaacgtccgg acggttagtc
    21541 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    21601 ttcagagtct gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct
    21661 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
    21721 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
    21781 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg
    21841 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    21901 cggctcggct cgcgcagata ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag
    21961 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
    22021 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    22081 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    22141 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
    22201 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    22261 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    22321 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    22381 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    22441 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    22501 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    22561 tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    22621 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    22681 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    22741 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    22801 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    22861 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    22921 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
    22981 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    23041 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
    23101 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    23161 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
    23221 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    23281 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    23341 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    23401 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
    23461 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    23521 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
    23581 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    23641 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
    23701 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    23761 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    23821 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    23881 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
    23941 accgaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
    24001 cagcttggct tcgatgtctt taatacggcc ttcgcagaaa ccctgctgtt cacgagctgc
    24061 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    24121 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    24181 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    24241 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    24301 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
    24361 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    24421 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    24481 attgaccagc tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca
    24541 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
    24601 tattgattac cacagtcagc agatggcgct gcccgccagt acgcagaaag tgattactgc
    24661 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
    24721 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    24781 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    24841 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    24901 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ctgccatagt
    24961 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt
    25021 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    25081 aggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggcgacc tgaaccgctt
    25141 tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
    25201 ggatggagcc agctatgccg gggcaattct gaaagatgag ttaaaacagg caggtatcac
    25261 ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
    25321 taaacagtcg gccccgttgc acgatctgct taagattatg ctgaaaaagt cggacaacat
    25381 gatcgccgat acggttttcc gcatgatagg ccatgcgcgc ttcaatgtgc ctggaacatg
    25441 gcgggccggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
    25501 aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
    25561 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
    25621 gctgccgctt gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    25681 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    25741 attcattacc acagcgagcg ggcaacgaat ggcgtttgta caatatcttt ctggctatgc
    25801 agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
    25861 gtataaagat atttatcaga acaattagtc aaaaagaaac cccggcacat ggccggggtt
    25921 ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg tcccagtcat
    25981 catcccagtc ctcatcatcc tcttcagcaa tctcttcaag ctgctggcga tgataatcat
    26041 cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg accgggtttt
    26101 caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtcca ctcgccgcag
    26161 agatcagata atatttatct tcccagccca gcgcttcagc aatcgctttc gctttctctt
    26221 cagcttctgc cttatccagc agatcgatct tattgaacac taaccaacgc ggtttcgccg
    26281 ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt tcaaccggat
    26341 cggtgccgtc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa cgttccaggt
    26401 gcttcaggaa gcggataccc agacctgcgc cttccgcagc gccttcaatc agtcctggaa
    26461 tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacacccaga cttggcacca
    26521 gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgcg cgaataaagg
    26581 tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc atcagctcca
    26641 gcagcagctc acgcttatca cccggcgtac cgttggtttt ctgccgtggt gtacggttaa
    26701 cggacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca accagcagac
    26761 gctgaccgtg tttagtcata tcgcccatgg tttcaccagt accttggtcg attatacgcg
    26821 tacctaccgg cactttaatc gtcacgtctt taccgcgctt accggtacaa tcgcggcttg
    26881 cgccgttctg accgcgctct gcacggaaag atttttcaaa acgataatcg ataagcgtgt
    26941 tcaggttttc gtcggcttcc atccatacgt caccaccatc accaccgtcg ccgccatccg
    27001 ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta ccgccatcac
    27061 ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc gtaaatcatt
    27121 cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc caaatacgat
    27181 gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa
    27241 ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa
    27301 acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag
    27361 ccatcgcttc cgccagggcg ccatatccta ccaatgtatt cagtccgcaa aaaattaaac
    27421 atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga gggaagagcg
    27481 caattgtaca taaagcgtac agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata
    27541 aaaccttttg cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc ccaaagataa
    27601 ctccccaggt gtaatcggtg agctttgtaa atatctcgac cagactggtg ttaaaaaaca
    27661 tcaccaggcc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc attttctctt
    27721 tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca atcacctgcg
    27781 aagcggtcgg actcaggtat tgcaaggatg agctgaacag gatgaagttc ccaaacagcc
    27841 cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg
    27901 gcaacctctt cttcaccgca agaatggcac caaggccaat actcgccatc aagaaacggt
    27961 aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg attggcaacg
    28021 ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca
    28081 taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc atttttaccg
    28141 gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc
    28201 gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct ttaacttcga
    28261 atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag
    28321 cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc agaacggatt
    28381 cgccaccgaa acgcttaacg cccaggcgtt tagcttctga atcgcgaccg ttacgtgtgg
    28441 agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg cgctgatgcc
    28501 agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat agtgtttacg
    28561 acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa cttcagcttt
    28621 gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt ttgcgatcat
    28681 cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt ccaggcgaac
    28741 ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg cgtacatata
    28801 aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa tattcacaat
    28861 agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca atacacgaag
    28921 aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt acaatcactc
    28981 tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa
    29041 agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca agatatggcg
    29101 ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccagctgat caatcagtta
    29161 ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc tgtactggct
    29221 gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct gatcgagttt
    29281 atccacacgg cgactctgct gcacgacgac gttgtggatg aatcagatat gcgcaggggt
    29341 aaagctaccg ccaacgctgc atttggcaat gccgccagcg tgctggtagg cgattttatt
    29401 tatacccgcg cgttccagat gatgaccagc ctcggttcgc tcaaagtgct ggaagtcatg
    29461 tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa cgttaacgat
    29521 ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt
    29581 gaggctgccg cgcagtgttc cgggattctg gctggctgta cgccggaaga ggagaaaggc
    29641 ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga tttactcgat
    29701 tacaatgccg atggcgaaca gttaggtaaa aatgtcggcg acgatctgaa cgaaggtaaa
    29761 ccgacgctgc cgctgctgca tgcgatgcat catggcacac cagaacaggc acagatgatc
    29821 cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct ggaagcaatg
    29881 aacgcttgtg gatctcttga atggacgcgt cagcgtgccg aggaagaagc agacaaagcc
    29941 atcgcagcgt tacaggtgct cccggacacc ccttggcgag aagcactcat cggcctcgcg
    30001 cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag cgcgcggggt
    30061 ggttccagta aattccataa aaacacttac tcagctctat aacacctgaa aaacaggctg
    30121 tgcatcttta tatattctga atattcacac tccttacagg aactttttag agcaatcggc
    30181 catcaggagt atagtgatgc tcgacagaag aagtgttctg aatgaaagcg actaacttaa
    30241 ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca tcattgcggg
    30301 tttgcgcaag aagggaacgt caatggcggc ggagtctcgc agaaatggtt tgagttcctc
    30361 aacgctggcg aatgcattat cgcgcccatg gccgaaagga gagatgatta ttgcgaaagc
    30421 cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc agacccatga
    30481 gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg
    30541 gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt tcacacgctc
    30601 aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg
    30661 ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca ccaggcttgc
    30721 tgatgcacgc agatcggttg ccataacctg tgcgccagaa agtttttcaa caccgtgaca
    30781 aataacggta ttgctttcga tttcggcatg cgcgcccata cggctaagct ccggcacatg
    30841 cataaagcgg ttttcaaaga ccgtttcggt gatgaatccg gtcccttctg ccaccaggtt
    30901 caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac
    30961 gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt cgccgacttc
    31021 gatgtccgct ccagcgtcac gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc
    31081 gttacggcag ataattttgc cgcgagaaat cgccgccgcc accaggaaag taccggtttc
    31141 gatacgatcc ggtagcacgc gatagacacc gccgcctaaa cgttccacac cttcgatgac
    31201 gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga agttcgcggt
    31261 atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtacctt ccgccagggt
    31321 tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca cgatatgtgc
    31381 gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt tgatggtcgc
    31441 gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc
    31501 gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca gtgcccagat
    31561 agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc
    31621 atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta gcagcttcat
    31681 tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt cttccgccag
    31741 tagagcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg tgacttcgcc
    31801 ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc tcagttaaca
    31861 attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg
    31921 cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt tatccgcaat
    31981 atattccatc agcgggccat agaccgtctg ctgtttttta acccgactca tgccgtcaaa
    32041 caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg
    32101 gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg gatcttcaat
    32161 catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat aaataagcaa
    32221 aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc ggacaggcgg
    32281 ttaacgccat atccggcctg aaaaaattta acgaggcaga acatcagcag gcaaattata
    32341 caatttcgcg agggtataca ctttgtcgtt taccccctga agcgtcacgt tgttgccctg
    32401 ctttttcgcc agatcgataa gatggagcag cagtgccagt ccacccgtat ccacgcggga
    32461 cacacggcta agatcgatgc aggtaatccc cttcaccgct tcctcacgca tttcccaaag
    32521 cggtagcaga acgtcctgat ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat
    32581 ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg ttgagaaatc
    32641 gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt tccccactcg
    32701 ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc ctgccagttt
    32761 cccgtctggg agtttttacg ccactggaag tccagacgca ccggcggacg gccattcggg
    32821 tcaataatgg taacgcgaat aggcacaatg gttttatcgc ccagcggctg ttctggcgca
    32881 atctgatagg tttgaccgtg atacatcgcc agcgcctgac cgtaagcctg cttcaggtac
    32941 tcacggaaag cggcaaagta ggcttcacgt tgagcagggg tcgcactctt gtaatactgg
    33001 cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca
    33061 atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt caggcgatcg
    33121 aacgttttct gcgccgcctc gtccatcagc ttatacggat tggtctggtc tgccgcggtt
    33181 gccgcactca gaggtgcaat caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt
    33241 cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt attacctggc
    33301 gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc gtaaaggaac
    33361 tgaccaatga gatcttccag caccatcgca gacttagtgt cctgaattgt atcgccatcc
    33421 ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc cagatattgt
    33481 tcccccagca ggccggaagt acgaatgctc agcgaactgg tatctggaat gtggttataa
    33541 cgttgttcaa tttccagcgt tacgcgtggc agataggttt tcgggtccag cgtaatatcc
    33601 gccacccggc ccacaacaac gccaccaata ctgaccggag agcgcgcttt caggccgcca
    33661 atgttatcga acgtcgcata aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc
    33721 gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg ctaataaaaa gatacccacc
    33781 caaatttcat tttttttcgt ttgcatgaac tcaattccca aacatcaatg cggtcagcac
    33841 aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg ttgcccggct
    33901 aatcccggca gacgtcggga tggcgtcgta gccgttaaac aacgaaatcc acgtcaccgt
    33961 gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc agtcgacggc
    34021 attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga cgccgaccag
    34081 agatccgccc cagatcccca cggcgacgaa aataaccgtc aacagtggta atgaaataac
    34141 cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca tcatctccat
    34201 actggagagt tgctctgtag cgcgcatcag gccgatttct gcggttagcg ccgaaccagc
    34261 acgcccggca aacaacaacg cggcaaccac cggccccagt tcacgcagta gcgataacgc
    34321 caccagcata cccagactgg tttccgcact ataagtggtc agaaccagat aaccttgcag
    34381 ccccaacacc attccgatga acacgccaga aaccacaata atcagcatcg acaggacgcc
    34441 gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac
    34501 cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt taatcccttt
    34561 atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct ggtaaaagat
    34621 cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct atcccgtcca
    34681 gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc ctgggcactg ccatgagcga
    34741 caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact tccggcacat
    34801 cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa atcagcttca
    34861 ccagtacgcc catggtgatg ggatcttgcc caacaaaagg ttcatcaaac atgatgagat
    34921 ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca ccagaaagtt
    34981 cagaaggcat tagcttagcc gctccacgca gccccacggc ctccagcttc atcatcactg
    35041 tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag gcgacgttgt
    35101 caaatacgtt catatcagtg aacaacgccc cggactgaaa taacatgctc atccgtttgc
    35161 gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg aaaaggatct
    35221 caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc
    35281 ccgatggccc catgattgcc gtgatcttcc ctcgcggcac ggtcagggaa atattatcga
    35341 agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa ttcgccacag
    35401 actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa
    35461 ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag ctggcatttg
    35521 ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt ctgaaaatct
    35581 tcagcggacc ggcgagtata cctgaagaaa ggatgttaga tgcttttagc tacggcactg
    35641 ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct
    35701 attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt ggtcagtatt
    35761 ggtacatcgt taccagaagt catcgtctcg cttgccgcgt ctctgcacga acaacgcgat
    35821 ttagccgttg gtacagccct cggctcaaac attatcaata tattgctgat cctcggtctg
    35881 gctgcgctgg ttcgtccttt taccgtccat tctgatgttc tacgccgtga attaccctta
    35941 atgttgttgg tcagcgtggt ggccggttcc gtactctatg acggacaact tagtcgcagc
    36001 gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt taaacttgca
    36061 cgtcaggctg aacgtcaggg gactgacagc ctgaccagag agcagcttgc agagctgccg
    36121 cgtgacggcg gattgcccgt cgcgttttta tggctcggca ttgcgcttat catcatgcca
    36181 gtggccacgc ggatggtggt tgataacgcc acggtgctgg cgaattactt tgccatcagc
    36241 gagttgacga tgggtctgac ggcaattgct atcggaacca gcctgccgga actggcaacc
    36301 gcaatagcgg gggttcgcaa aggtgaaaac gacattgctg tcggaaatat cattggcgca
    36361 aacattttta atattgtcat cgtgttgggt ttacccgcgc tgataacgcc aggagagatt
    36421 gatccactgg cgtacagtcg tgactacagc gtgatgttgc tggtgagcat tatttttgcg
    36481 ttgctgtgct ggcggcgctc cccacaaccg ggccgtggtg taggggtatt attaactggc
    36541 ggatttatcg tatggctggc gatgttgtac tggttatcgc caatactcgt tgaataactg
    36601 gaaacgcatt atgtcgcacg tagagttaca accgggtttt gactttcagc aagcaggtaa
    36661 agaagtcctg gcgattgaac gtgaatgcct ggcggagctt gatcaataca tcaatcagaa
    36721 tttcacgctt gcctgtgaaa agatgttctg gtgtaaaggg aaagttgtcg tcatggggat
    36781 ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg tttgccagca ccggtacacc
    36841 ttcatttttc gtccatcctg gtgaagccgc gcatggtgat ttaggcatgg tcaccccaca
    36901 ggatgtggtg attgctatct ctaactcagg tgaatccagc gaaatcacgg ccttaattcc
    36961 agtgcttaag cgtcttcacg taccgttaat ctgcatcacc ggtcgcccgg agagcagcat
    37021 ggcgcgcgct gcagatgtgc atctgtgtgt taaagtagcg aaagaagcct gtccgttagg
    37081 gctggcaccg accagcagca ccaccgccac gctggttatg ggcgatgccc tcgctgtcgc
    37141 gctgttaaaa gcacgcggct ttactgctga agattttgcg ctctcacacc caggcggcgc
    37201 actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg catacgggcg atgagatccc
    37261 gcatgttaag aaaacggcca gtctgcgtga cgcattgctg gaagttaccc gcaaaaatct
    37321 tggtatgact gtcatttgcg atgacaatat gatgattgaa ggcatcttta ccgacggtga
    37381 tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag ttaagtattg ccgatgtgat
    37441 gacgccgggg ggaatacgtg tgcgccctgg cattctggcc gttgaggcac tgaacttaat
    37501 gcagtcccgc catatcacct ccgtgatggt tgccgatggc gaccatttac tcggtgtgtt
    37561 acatatgcat gatttactgc gtgcaggcgt agtgtaaaga ttcaaggata aacaacaatg
    37621 agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg tcagcgccga cgttatggca
    37681 aaagcagaga acattcgtct gctgatcctc gacgttgatg gcgtactgtc agatggcctg
    37741 atttatatgg gcaataacgg cgaagagctg aaagcgttca atgttcgtga cggttatggc
    37801 attcgttgtg tgctcacctc tgatattgaa gtcgctatca ttaccgggcg aaaggctaaa
    37861 ctggtagaag atcgttgtgc cacattgggg atcactcact tgtatcaggg gcagtcaaac
    37921 aaactgatcg cctttagcga tttgctggaa aaactggcga ttgccccgga aaatgtggct
    37981 tatgtcggcg atgatctcat cgactggccg gtaatggaaa aagtgggttt aagcgtcgcc
    38041 gtggccgatg cgcatccact gttgatcccg cgcgccgatt acgtgacgcg cattgctggc
    38101 ggtcgtggcg cagtgcgcga agtttgcgac ttattactcc tggcgcaggg caaactggat
    38161 gaagccaaag ggcaatcgat atgagtaaag ccagacgttg ggttatcatt gtgctatcac
    38221 tggcggttct ggtgatgatc ggcattaata tggccgaaaa agacgatacc gcccaggtgg
    38281 tcgtcaacaa caatgatccc acctataaaa gcgagcatac ggacacgctc gtctataacc
    38341 cagaaggggc actaagctat cgattgattg ctcaacacgt tgaatattat tccgatcagg
    38401 ccgtttcgtg gtttacgcag ccggtactta ccacgtttga taaggataaa atcccgacat
    38461 ggtccgtaaa agcagataaa gccaagctga ccaatgaccg gatgctctat ttatatggac
    38521 acgttgaagt caacgcactc gtgccggact ctcaacttcg cagaatcacg acggataacg
    38581 cgcagatcaa tctggtgacg caggatgtta cctctgaaga cctcgtcacg ttatacggaa
    38641 caacatttaa ctccagcggt ctgaaaatgc gcggcaactt acgcagcaag aacgccgagc
    38701 tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa acaaactcag ccttaatctt
    38761 gtgcttgcca gctcacttct ggccgccagc attccggcat ttgccgtaac cggagacact
    38821 gatcagccga tccacattga atcggaccag caatctcttg atatgcaagg caacgtggtt
    38881 acctttaccg gtaatgtcat cgtcacccag ggcaccatca aaattaatgc cgacaaagtg
    38941 gttgttaccc gtccgggcgg cgaacaaggt aaagaagtga ttgacggcta cggtaaaccg
    39001 gcaacgttct accagatgca ggacaacggt aaaccggttg aaggtcacgc ttcccagatg
    39061 cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta atgcttatct gcagcaggtt
    39121 gatagcaaca ttaagggcga taagatcact tacctggtaa aagagcagaa aatgcaggct
    39181 ttcagcgaca aaggcaagcg cgtaaccacc gttctggtgc cgtcgcagct gcaggacaaa
    39241 aacaacaaag gccagacccc ggcacagaag aagggtaatt aattcgttat ggcaacatta
    39301 actgcaaaga accttgcaaa agcctataaa ggccgtcgcg tggtagaaga cgtcagcctg
    39361 accgtcaact ccggggaaat tgtcggtctg ctggggccaa acggtgccgg taagaccacc
    39421 actttctata tggttgtagg cattgtgccg cgcgatgcgg gcaacatcat tattgatgat
    39481 gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc gcggtatcgg ctatctgcca
    39541 caggaagcct ccattttccg tcgcctcagc gtttacgata acctgatggc ggtactgcaa
    39601 attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg cgaacgagct gatggaagag
    39661 tttcacattg agcacctgcg tgacagcatg gggcagtcac tctccggggg tgaacgtcgc
    39721 cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat ttattctgct cgacgaaccg
    39781 tttgccggtg ttgacccgat ctcggttatc gacattaaac gcatcattga gcacctgcgc
    39841 gacagcggcc tgggcgtgct gatcactgac cacaacgtgc gtgaaacact ggcggtttgt
    39901 gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc acggcacgcc tacagaaatc
    39961 ttacaagacg aacacgttaa gcgtgtatac cttggggaag acttcagact ctgatagggt
    40021 agaagtttgc gacgttttag caggagagta cgattctgaa catgaagcaa ggtttgcaac
    40081 tcaggcttag ccaacaactg gcgatgacgc cacagctcca acaggcaatt cgtctgttgc
    40141 agttgtcgac gctggaactt cagcaggagc tacagcaggc gctggagagt aatccgctgc
    40201 ttgagcaaat cgacactcat gaagaaatcg acacccgcga aacgcaagac agtgaaacgc
    40261 tggacaccgc cgatgcgctc gaacaaaaag agatgccgga agagctgccg ctcgatgcca
    40321 gttgggacac catttacacc gctggtacac catccggcac cagcggtgac tacattgacg
    40381 atgagctgcc ggtctatcag ggcgaaacga cgcagacctt gcaggattac ctgatgtggc
    40441 aggtcgagct gacaccgttt tccgacactg accgcgctat tgctacctct atcgtcgatg
    40501 ccgttgatga caccggttat ctgactgtcc cgctggaaga tattctcgaa agtatgggcg
    40561 atgaagagat cgacatcgac gaggttgaag ccgtccttaa gcggatccaa cggtttgatc
    40621 cggtcggtgt agcggcaaaa gatctgcgtg actgcctgct aatccaactc tcccaattcg
    40681 ataaaaccac gccgtggctg gaagaggcca gattgatcat tagcgatcat ctcgatctgt
    40741 tagccaatca cgacttccgc actttaatgc gcgtcacgcg tctgaaagaa gatgtgctga
    40801 aagaagccgt caatctgatc cagtcgctcg atccgcgccc cgggcagtcg atccagactg
    40861 gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa gcataacggt cactggacag
    40921 tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa ccagcactac gcctcgatgt
    40981 gcaataacgc tcgcaatgat ggtgacagcc agtttatccg cagcaatctg caggatgcca
    41041 aatggttgat caagagtctg gaaagccgta acgatacgct actgcgcgtg agtcgctgta
    41101 tcgttgaaca gcagcaagcc ttctttgagc aaggcgaaga atatatgaaa ccgatggtac
    41161 tggccgatat cgcccaggct gtcgaaatgc atgaatcgac gatatctcgc gtgaccacgc
    41221 aaaaatacct gcatagtcca cgaggcattt ttgaactgaa gtatttcttt tccagtcacg
    41281 tcaataccga gggcggcggc gaagcttcct ccacggcgat tcgtgcgctg gtgaagaaat
    41341 taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag caagttaacc tctttgctgt
    41401 cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa gtaccgagag tctttatcca
    41461 ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa ccgataagga agacactatg
    41521 cagctcaaca ttaccggaaa taacgtcgag atcaccgagg cactgcgcga atttgttaca
    41581 gccaaatttg ccaaacttga gcaatatttt gaccgaatca accaggtcta tgttgttctg
    41641 aaagtggaga aagtcaccca cacctcagat gcaacactgc atgtaaacgg tggtgaaatt
    41701 catgccagcg cggaaggtca ggacatgtac gctgccattg atggcttaat tgataagctg
    41761 gcacgccagt tgaccaaaca taaagataaa ctgaaacaac actaattgtc cgggcaatta
    41821 gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat ttgtacggtt aatgctccga
    41881 gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa ttatgacaaa taatgataca
    41941 actctacagc ttagcagtgt tcttaacagg gaatgtacgc gaagccgcgt ccactgtcag
    42001 agcaaaaaac gcgccctgga aatcatcagc gagctggcgg cgaaacaact tagcctgccg
    42061 cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa tgggcagtac cggtatcggc
    42121 aatggtattg ccattccgca tggcaaactg gaagaagata ctctgcgcgc cgttggcgtt
    42181 ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg acaaccaacc ggtggactta
    42241 ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc acctgcatac tctgtcgctg
    42301 gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt tacgcgcagc ccagagcgat
    42361 gaagagctgt atcaaatcat tacggatacc gaaggtactc cggatgaagc gtagttattc
    42421 ggtaatgtct cttttagacg ttgtgaggag aaacagtaca tggtactgat gatcgtcagc
    42481 ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc tggaagatat gggtttttac
    42541 tgcgtggata accttcccgt agtgttgtta cccgatctgg ctcgaactct ggccgatcgc
    42601 gagatttctg ccgccgtcag cattgatgtt cgtaatatgc cggagtcacc agaaatattc
    42661 gaacaggcga tgagtaacct gcctgacgct ttctcaccgc aactactgtt cctggatgcc
    42721 gaccgtaata ccttaattcg tcgttacagt gacacgcgcc gactgcatcc gctttccagc
    42781 aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg atttgctgga gcctctgcgt
    42841 tcgcgagcgg atctgattgt cgatacctca gaaatgtccg ttcacgagct ggctgaaatg
    42901 ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga ccatggtctt tgagtctttc
    42961 ggcttcaaac acggtatccc tatcgatgca gattacgtct ttgacgtgcg cttcttgccg
    43021 aacccgcact gggatccgaa actgcgtcca atgacaggtc ttgataaacc tgtcgccgcg
    43081 ttcctcgacc gccacacaga agtacacaat tttatctacc agacgcgaag ctatcttgaa
    43141 ctatggttac ctatgctgga aaccaacaac cgtagctact tgacggtcgc cattggttgt
    43201 accggcggaa agcaccgttc ggtgtatatt gcagagcaac tggcagacta cttccgctcg
    43261 cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa aacgtaaacc atgaccgtca
    43321 agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc ccggcctgca atgaagctgt
    43381 ttgaattaat gcagggtttt gacgctgaag tgctgttacg taatgacgaa ggcaccgagg
    43441 ccgaagccaa cagcgttatt gcgctgctga tgttggattc tgccaaagga cggcagattg
    43501 aagttgaagc gaccggccca caggaagagg aagcactggc cgccgttatc gccctcttta
    43561 actctggttt tgatgaagat taatcttcat cactttgaca tacaactccc ttcaaactcc
    43621 ccccgacaat aagaaaatca cgtactgaaa tcgttctcaa tcaacgtcat ttgtacattt
    43681 tgtgcgcttt tcactttcag aagaacctta agaaaacctt aagagacatt gtttaggttt
    43741 tgtttaagtt aatcgaccat actggagatc gtcagaaaat atttccagga gatggcatga
    43801 ttcgcttatc tgaacaaagt cccctgggca ccggaagaca tcggaaatgt tatgcgcatc
    43861 cggaagatgc ccaacgctgt atcaagattg tctaccatcg tggcgatggt ggcgataaag
    43921 agatccgccg tgagttaaag tactacgcgc atcttggtcg ccggttaaaa gactggagtg
    43981 gaataccgcg ctatcacggt accgttgaga cggattgcgg aaccggttat gtctacgatg
    44041 tgattgcaga ttttgacggc aaaccctcca ttacgctgac cgaatttgct gaacaatgcc
    44101 gttacgaaga agacattgca caactgcgcc agctactgaa acagctgaag cgttatttgc
    44161 aggataaccg tatcgtgacg atgtcgctga agccgcagaa tatcctctgt caccgcatca
    44221 gtgagtcaga ggttatcccg gtcgtctgtg acaacattgg tgaaagcaca ttgattccac
    44281 tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga gcggttatgg aaacgattta
    44341 tcgcccaacc ggcgctggct attgccctgc agaaagatct gcaacctcgt gagagcaaaa
    44401 cgctggccct cacttcgcgc gaagcttaat ccagctggtg ttgttgcata aacggctcac
    44461 cgcctaactg atacatctgc cgtaaaatcc acgcctgacg gctacgcacg tagcccgatg
    44521 gcgaggagac tttgaaacga agtggattag gtaatacagc tgccagtaat gcagcttccg
    44581 accgggtaag tttgctcgcg ggtttgtgga aataacgttg tgccgcagct tcgacaccaa
    44641 acacaccgtc gccaaattcg gcgatattca ggtaaaccgt caggatacgc tttttgctcc
    44701 agactgtttc tatccccagc gttaatccag cttccagccc ttttcgtacc cagctacgcc
    44761 catcccataa aaagagattt ttggccgtct gctgagaaat cgttgaagca ccgcgaatac
    44821 ggttttcatt gcgctcgttg tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc
    44881 agtgctcagg aaatttctga tcttctgcgg caatcaccgc cagtcccatc cacggcgaga
    44941 tttgatccat actgacccag tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg
    45001 cgctgacctg tcgctcgacc attaccgctg agaagggaac aggcgcaaca ctaaacaacg
    45061 cgatgccccc gccccagaaa acggcgagga caaccattaa ccgcaaaaga aaacggcgaa
    45121 cgaaactaaa caccgttaag cggcttttac tcattcagcc agaaccagca cgcgggaaac
    45181 cagcttatca atgccgctcg ccgcttctgc aatgttctgc gccagcatat atgctggggt
    45241 ggtgacaatc ttattgtctt catcaaccac gatatcatcg acaggacagg gcacatgctc
    45301 cgcgcccatc tcttccagca cttctgcggt atcgatatca gtaccgatgg tcaaacgcag
    45361 cgggaaatcg aaaattttcg gcagcatcgc cggggcaata cacataaaac caagcggttt
    45421 tccggcctga tgcatcgctt gtgccagcgc ctttaattca cggtcaacgg tgcattcgct
    45481 accaagagtg gcaaaattgc ttaaattctt cgccgcgcca aacccccccg gcacaatcaa
    45541 cgcatccagt tcagcggcat cggcctgggc cagaggacgg atttcaccgc gcgttattcg
    45601 tgccgcttca atcagcacgt tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat
    45661 aacatcaacc tgctgcttat ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat
    45721 agctaacaac gtcaacaccg cttcatgaat ttcagaaccg tcatagacgc cgcatccgct
    45781 cagaattacg ccaattttct tcattgtgat catcctttcg caattgactg aaacacatta
    45841 atttttttaa taaaaatggt acgcatcaca catttaactg attcatgtaa caaatcattt
    45901 aagttttgct atcttaactg cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat
    45961 cataataatt tacggcgcag ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc
    46021 ggtctagccg gggtcatttt ttagtggctt ttgccaccca cgctttcagc acttctacgt
    46081 cgtgacgcca ctcttctttc atctcttcaa tccattcacc gacgttatct tcccaggcag
    46141 gaaggtccgg agactgaatt tgctgaccca gctgttgcag atggcgtaac cccactgacc
    46201 ccgccgcacc tttaatttta tgtccttcct ctacaatgcc ttttttatcc tgcgccgtca
    46261 gattcgactc cagcacgctg acatagcccg gcatcatctt ctcaaacacc gctaacccgt
    46321 cggtgatcag cttcggtcct acaagttcga gatactgttc cagcatggga atatcgagca
    46381 atgcttctga tttactgttc tcttctgtcg tcaccgtact ctcctcatca tcctgggtat
    46441 cccagaattt cttgatcatc gcggttagcg ccggaaccga aagcggctta ctcagcacat
    46501 catccattcc agcattgagg tactcttgtt tgtctttcag cacgttagcg gttaaggcca
    46561 ccagcggcgg taaatcctcg cgcggataac gcttcgtcag ttcacgagag atatccagcc
    46621 cggtcatatc tggcaactga atatccagca acaccaggtc gtattcgccc ggtttaaaca
    46681 tctccagcgc cgccttgccg gtcatggcga catcaacgct gttacctaat ttttccagca
    46741 cagaacgcgc aacaatcacg ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg
    46801 gtaaaggcat atcgtcttca tcaaacgcat catcgacctc ttctgctacc gacggtgcgt
    46861 ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct ggtaaccgta atatcgccgc
    46921 ccatattttt cgccagacga cgagaaacgg ccagaccaat accggtgccg gtggcaggtt
    46981 taccgccatg actgtctttc acctggtaat acatggcgaa aattttatcc agctcatcct
    47041 gcggaatgcc gataccagag tcttccactt caaaatgcag catatcgcct tcatcgtagc
    47101 gcacgcgcac ggtaacctgg ccttgctggg tgaatttgac ggcgttactg atgaggttcc
    47161 acaggatctg ccgtaaacgc gtcccgtcgg taatgacctg atgcggtaat ggcagcgtcg
    47221 gctccaggtt aaagcgcaat cctttttgtt gcgcctgcaa tgcggagaga ttttccagat
    47281 cggcaaggaa gctggtgaaa tcaaccggtt gattatcaag ctggaccttg cgccgttcca
    47341 tcttatccat gtcgataata tcgttaaaga tattccccag cgtgacggcc gaaacatgga
    47401 tggtcttgag atatttttcc tgctcggcgg tgagttcggt atccagcaga atacggctca
    47461 ggccgacgat accgttcagc ggtgtacgca attcgtgact gatggtggag ataaacgtcg
    47521 ttttgtcgcg gctggcccgt tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc
    47581 gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc gtagtacggc actttacgga
    47641 tttcaaagca ggctttgcgc ccgtccgggt aatccagcca ctgttcatag gtcagtgaca
    47701 cattatgacg gaacactttt tcatcggttt caatgacttt tgcggcggct tccggtgagt
    47761 aaacatcagc aggtttcagg tgaaccagtt gtttttcgct ttttccggtc agcagctcca
    47821 tcgcgcggtt acagccggaa aactctttat cttcgttacg ataaaaaacc aggtcgggtg
    47881 aagcatcaag gaaggaacgt aagaatgagg attgctgctc gagctgaatt tgtgtctctt
    47941 cgcgctcttt gatttcaatt ttcagttggc cgaaggtttc ctgcagttct gcttccgctt
    48001 tttcacgaac ggcgatttcc tgatttagct gggcaatatt atcttttaac tgaacgttga
    48061 ggctcaaatc gcgctcgcgc atctcctcca gtttttgcac cagccgtgac agacgttgtc
    48121 gtgactcctc cagttgctcg acgaccaccg atagaaagta gaccgcccac ggcgtaatca
    48181 gcaaaccaaa gaagatagaa cgaataacat caatgctttc gacctgacca tgcagcacca
    48241 tggttaccgc catttgtacc acaatggcaa gaacgacgag ggccagcgcc agcaacattg
    48301 agaagcgcac cagacctaac ttcatcatca ggtcaacata atactgcgcc agcagacgaa
    48361 tttgcttcat tagggaattc cttcacgaca acctgcccca ataataccca attctgacag
    48421 ttacgttata aattgtgtga gaaatgcgga attcccctca acgacctact ccgttggagg
    48481 tatccacgga cgttccagcg ccgatccctg tacgccatgt tcattcagat agcgatccag
    48541 ctcgaccatt cccgtccatc gattttcgca ccacagagga gcaagcagcg tcgggcgacg
    48601 ggcgctggcg gaaatgcggt ggtagatcac ttccggtggc gtatggcgaa tcatttctcc
    48661 ggcagtgagc gtgtaatcct ccagttcaat accgttcaaa cgccccgctt cccaggcttt
    48721 cgccataatg ctgcctttca caatatgcag cggatgcagc tttatgccgt ccacgccggt
    48781 ttcaaccact cgctccagcg tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac
    48841 aattaagtgg gaacacacct tcagcccgcg ctgacgtgcc agctgggttg tacgctgata
    48901 acaggcaaaa tcatggccgc ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag
    48961 ccccagctcc agccacactt cgtaaccctg gtccttatat tcgcaaagca gatccagcac
    49021 cgcatccggc acgcagtccg ggcgggtacc aacacacaaa ccgacaatat tggcctggct
    49081 caccgcctgc tgatacatag aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc
    49141 ctgaaagtag gccagatagc gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg
    49201 ctcggcaatg gaacgatgct gctgcgcttc atcggcaaac gaggcaacat tacagaatgt
    49261 gcagccacca cgcccgatgg taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt
    49321 cagcttatgc accttttgcc cataacgacg ggtgagatca ccaccaaaca tattgactaa
    49381 tttctgtaac tgcataatct gatagaccgc gccttgaaaa gaggccaaag cctgccattt
    49441 ttagctcaat tcggcgatga cctggatcaa tcgtcccggc ctgcttttat caactgcata
    49501 atcaatcaaa attaccgaaa tttcatgcat aatcacataa atcacttttg cttatcttgt
    49561 gtcagatttt tttatctcct tatggatttt aggcaaaaac agtagcatga aacgccatta
    49621 ccaattaagg cagtataaaa tgctggcttt ctcgtcagtt caaggcagga taagggttaa
    49681 cacgccttta tgacagtcag gaattgactg tttctctaac gacttccctt ttagccttaa
    49741 agataaaatc tattttaatt tcagtcattt aataaagaat tttgcgctaa agcacatttc
    49801 tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg gaaatccgct
    49861 ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga gatcccctct
    49921 tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga
    49981 cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg gaagtggggt
    50041 tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt gagagggata
    50101 actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag gtagtgcgta
    50161 ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa
    50221 ccggcgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc
    50281 aggagcgcgg ctggcgttta gcaaaaaact acgccgtcgg gatgctattc ctgaataaag
    50341 atcctgaact cgccgctgcc gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct
    50401 tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc
    50461 tctcctctct gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc tggcgtccgc
    50521 gcgatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg
    50581 acaaagactt ctacgtctgt agcctgtcga atctggtgaa catctataaa ggtctgtgta
    50641 tgccggcgga tctgccgcgc ttttatctgg atcttgcgga cctgcgtctg gaatcggcca
    50701 tctgcctgtt ccaccagcgc ttctccacta acaccgtacc gcgctggccg ctggcgcagc
    50761 cgttccgcta cctggcgcat aacggtgaaa tcaacaccat caccggtaac cgccaatggg
    50821 cgcgtgcgcg tacctataaa ttccagacgc cgcttatccc tgatctgcac gacgccgcac
    50881 cgttcgtcaa cgaaaccggc tctgactcca gttcgatgga taacatgctg gaactgctgc
    50941 tggcaggcgg gatggatatc atccgcgcca tgcgtctatt agtaccaccc gcctggcaga
    51001 acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc atgcatatgg
    51061 agccgtggga tggcccggcg ggcatcgtga tgtccgatgg tcgcttcgct gcctgtaacc
    51121 tcgaccgtaa cggtctgcgt ccggcacgct acgtcattac caaagacaag ctcatcacct
    51181 gcgcctctga agtcggtatc tgggattatc agcctgacga agtggtcgaa aaaggccgcg
    51241 tcgggccagg cgaactgatg gtgatcgaca cccgcagtgg gcgtattctg cactcggcag
    51301 aaaccgatga cgatctgaaa agccgccatc catataaaga gtggatggag aaaaacgtcc
    51361 gccgactggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc gaactggacg
    51421 acgacacgct tgccagctac cagaaacagt ttaactacag cgcggaagag ctggactccg
    51481 taattcgcgt actgggcgaa aacggtcagg aagcggtcgg ttcgatgggc gatgataccc
    51541 cattcgccgt gctctccagt cagccgcgca ttatttacga ctacttccgc cagcagtttg
    51601 cccaggtgac taacccgcca atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca
    51661 ccagtatcgg tcgtgaaatg aacgtctttt gcgaagcaga gggccaggcg caccgtttaa
    51721 gctttaaatc gccgattctg ctctactccg atttcaaaca gctcacgacg atgaaagagg
    51781 agcactaccg cgcagatacg ctggatatca cctttgacgt cactaaaacc acgctcgaag
    51841 cgacagtcaa agagctgtgc gacaaagccg aaaaaatggt acgtagcggc accgtgctgc
    51901 tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccagcc ccgatggcgg
    51961 ttggcgcgat ccagacccgt ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg
    52021 tcgaaaccgc cagcgcccgc gatccgcacc acttcgccgt gttgctgggc ttcggcgcga
    52081 cggctattta tccatacctt gcctatgaaa cgctgggccg cctggtagac acccatgcga
    52141 ttgccaaaga ttatcgtacc gtgatgctca actaccgtaa cggcatcaac aaaggcttgt
    52201 acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt
    52261 ttgaagcggt cggtctgcac gatgatgtag tgggcctgtg cttccagggg gcggtcagcc
    52321 gcattggtgg ggcgagcttt gaagacttcc agcaggatct gctgaacctg tcgaaacgtg
    52381 cctggctggc gcgtaagccc atcagccagg gcggcctgct gaaatacgtc cacggcggcg
    52441 aataccacgc ttacaacccg gacgtggtgc gcacgctgca acaggcggta caaagcggcg
    52501 agtacagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca accacgctgc
    52561 gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg
    52621 caagcgaact gtttaaacgc tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg
    52681 aagcccacga ggcgctggcg gaagcgatga atagcatcgg cggtaactcg aactccggtg
    52741 aaggcggcga agacccggcg cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg
    52801 cttctggtcg cttcggcgtt actccggcgt atctggtcaa tgccgacgtc attcagatta
    52861 aagtcgccca gggcgcgaag ccgggcgaag gcggtcagtt gccgggcgac aaagtcactc
    52921 cttacatcgc caaactgcgc tattcggtgc ccggagtgac gctgatctcc ccgccgccgc
    52981 accacgatat ctactctatc gaggacttag cgcagctcat tttcgacctc aagcaggtta
    53041 acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgtaggc accatcgcga
    53101 ctggcgtggc aaaagcttat gccgatttaa tcaccatcgc aggctatgac ggtggcaccg
    53161 gcgcaagccc gctgtcgtca gtgaaatacg caggttgccc gtgggagctg gggcttgttg
    53221 aaacccagca ggcgctggtt gccaatggtc tgcgtcataa gatccgtttg caggtcgatg
    53281 gcggcctgaa aaccggtgtt gatatcatca aagcggcgat tctcggcgca gaaagcttcg
    53341 gcttcggcac tggcccgatg gtggcgctcg gctgtaaata tctacgtatt tgccatctga
    53401 acaactgcgc aacgggtgta gcaactcagg atgacaaact gcgtaagaac cactatcacg
    53461 gcctgccgtt caaggttacc aattactttg aatttattgc ccgtgaaacc cgcgagctga
    53521 tggcacagct gggcgtaacg cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag
    53581 aactggacgg tttcaccgcc aaacagcaaa aactggcgct gtcgaagctg ctggagaccg
    53641 ccgaaccgca tccaggtaag gcactctact gcaccgaaaa caacccaccg tttgataacg
    53701 gcctgctgaa cgcgcagttg ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca
    53761 aaaccttctg gttcgatatt cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct
    53821 atatcgccca gacgcacggc gatcaggggc tggcagccga tcctatcaaa gcgtacttca
    53881 acggcaccgc aggccagagt ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga
    53941 ccggggatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc gccattcgtc
    54001 ctccggttgg ttccgccttc cgcagccatg aagcaagtat tatcggcaac acctgcctgt
    54061 atggcgcgac cggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc
    54121 ggaactccgg tgctatcacc gtggtagaag gcattggcga caacggctgt gaatatatga
    54181 cgggcggtat tgtctgtatt ctgggtaaaa ccggcgttaa cttcggtgcg ggcatgaccg
    54241 gcggcttcgc ttacgttctc gatgaaagcg gtgatttccg caaacgcgtt aacccggaac
    54301 tggtcgaggt cttaagcgtt gacgatctgg cgatccatga agagcatctg cgcggtctta
    54361 tcaccgagca tgttcagcat actggctctc agcgcggtga agagattctg gcgaactggt
    54421 caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgta aaagcactgc
    54481 tgggtcatcg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa
    54541 caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg ccaaagaaac
    54601 cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gccgttttcc gaaggccagg
    54661 ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag tggaaatgtc
    54721 cggtacacaa ctacatcccg aactggctga agctcgccaa cgaggggcgt atttttgaag
    54781 cggcggaact gtcgcaccag accaaccccc tgccggaagt ttgcggacga gtctgcccgc
    54841 aagaccgtct gtgcgaaggt tcctgcactc tgaacgatga gtttggcgcg gtgaccatcg
    54901 gcaacattga gcgttatatc aatgataaag cgttcgaaat gggctggcgt ccggatatgt
    54961 ccggcgtgaa gcaaaccggt aaaaaagtgg cgattatcgg cgcaggcccg gcaggtctgg
    55021 cgtgtgcgga tgtcctgacg cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag
    55081 aaattggcgg gctgctgacc ttcggtattc cggccttcaa gctggaaaaa gaggtaatga
    55141 cgcgtcgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat accgaagtgg
    55201 gccgcgacgt gcagctggac gatctgctga gtgattacga tgccgtcttc cttggcgtcg
    55261 ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag
    55321 cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggtgaa acccgcgacg
    55381 aaccgttcgt cagcatggaa ggcaaacgcg tggtggtcct tggcggtggc gacactgcga
    55441 tggactgcgt gcgtacgtcc gtgcgccagg gagcgaagca cgttacctgt gcctatcgtc
    55501 gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg
    55561 tagagttcaa attcaacgtc cagccactgg ggattgaagt gaacggtaac ggtaaagtca
    55621 gcggcgtaaa aatggtacgt accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg
    55681 cggagatcgt tgcaggttcc gaacatatcg ttccggcaga tgcagtgatc atggcgtttg
    55741 gtttccgtcc acacaacatg gaatggctgg caaaacacag cgtcgagctg gattcacaag
    55801 gccgcatcat cgccccggaa ggcagcgaca acgccttcca gaccagcaac ccgaaaatct
    55861 ttgctggcgg cgatatcgtc cgtggttccg atctggtggt aaccgctatt gccgaaggtc
    55921 gtaaggcggc agacggtatt atgaactggc tggaagttta agctctgtcc cagcgccgcc
    55981 tcctcagaaa agagtcggcg ctgaaattct cctttctttc tggaaagcgc ctcgcataat
    56041 ctgaagaaaa ccaatgtaat acgctcatct ggattgttac cggtagatgc ccggcgtata
    56101 gctgattcca ttattcaacg cagcgccata taaagggaca gataatggaa tcgctctcag
    56161 agagaacctc aacaggctac cagcaaatcc acgacggtat tattcatctg gtcgatagcg
    56221 cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac tgggaaattg
    56281 gccgacgaat tgtcgaattt gaacaaggtg gcgaggccag ggctgcgtat ggtgcgcagc
    56341 taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc tctgcaaaaa
    56401 acttacgcca aatgaggctt ttttacctct tttttcaaca tgttgaaatt cgccagacag
    56461 tgtctggcga attaacacct gccctggtcc acctacgtcc gtttactttc cgttaaaaac
    56521 gctgacgccc gcagctttta tgaaaaagag acgctccgct gtggctggtc tgttcgccag
    56581 ctagaacggc aaattgcgac ccaattttat gagcgaacac tactgtcaca tgacaaatca
    56641 gccatgctgc aacaacacgc tcctaccgag acgcatattc ttccgcaaca ggcgatacgc
    56701 gatccctttg tgctcgaatt tctggaattg aaagatgaat attcagaatc tgatttcgag
    56761 gaggcgctga tcaaccacct gatggatttc atgctggaac ttggggatga ttttgccttt
    56821 gttggtcggc agcgaaggtt acgcattgat gacaactggt ttcgtgtcga tctgctgttt
    56881 ttccaccgcc gtttacgctg cctactaatc gtcgatctaa aagtgggcaa attcagctat
    56941 agcgatgccg gacagatgaa tatgtatctc aactacgcca aagagcactg gacgctaccg
    57001 gatgaaaatc cgcccatcgg tctggttctc tgtgcagaga aaggagccgg agaagcacat
    57061 tatgctctgg caggtttgcc taacaccgtt ctggcaagcg aatataagat gcaactacct
    57121 gatgagaaac gactcgcaga tgaactcgtt cgaacacagg cggtgctaga ggaaggctat
    57181 agacgccgtt aatttcagcg gtttcgaatt ctgcagacag tgtctgcaga atctttaaaa
    57241 cgaactaagt tttaagccat taaatcagcc ttaaccttca ccacaacctt tttaatctct
    57301 ccaggctcgc cgacaacgca tcctggttta tgcggttcac ctggcataaa cacggcgaac
    57361 attcccggtt ttaagatgat ggcttgctcg ttatcaatgg tgctgcaaag ctgataatca
    57421 tcctcatggt ggaactcttc acactggcgc gcagtgcctg ccatgccaaa cagaatccgt
    57481 tcctcaccgt ttaataacag ttggatatca atgtattgct cgtgcaattc cgcttttttc
    57541 tcgacgggcg attgagtgtt aaacgtcatg acattcataa agatattgtc gccctgtaat
    57601 tcgtaacgac ccggcgcttt ttcttgcggt ctggcagcta atgccagcgt taacgcgtcc
    57661 tgtaacgcag gatgtaaccc agcagacggt aatgactgta cttcacccat catcataatt
    57721 tttctccctg ggccaacagc gcagccccaa gtaaacctgc atcgtggcgg tagtgcgccg
    57781 ccagtaaatc aacatgaaat gccgctggct cctgcgccag atacgtttcc accagcgcca
    57841 gatacccttc tgccagccca acgctgccac cgaccaccac gcactggcaa tcagttgtgg
    57901 ctttaatatc agcgatcagc cttgcaagcg tacgtgcgga gcggtgaatc agctgctgcg
    57961 cctgctcgtc gccctgcccg gcgcgtgtga aaatagttct cgcatccgcg cccgccagtt
    58021 caccctgcgc tgccgccgca atgccgcgac cagaagcaat cgcctccacg caacccgtgc
    58081 gtccacagcc acagaccggg ccgtgtggat cggcaagcgt atgcccgatg tgccccgcca
    58141 gaccgccagg gccggtaagc agtttgccgc cgctcactac accgccgccg acgccagtgg
    58201 aaacggtgat aaagaccata tcggttatat cgccttccag cgcctgatac tctgcccatg
    58261 ctgcggcctg cgcgtcgtta atggcaatgg tcggcaaatt ggtaagttgt tccagcgttt
    58321 tgactaacgg aaagtgtagc aatccaccga gattatgcgg attcagcgcc agcaaactgc
    58381 cgtcgcgaat aattccggtt gaagcgatgg caacccgctg cgcatgagct tgcaacggag
    58441 agactaatgc ggataaggca tcacgcaagg cttctggtgt ctggctggct ggcgtaggaa
    58501 gttcacgacg atcgcggatc tgcccgtcag cgccaatcag cgcggcggca agtttagtac
    58561 cgccgatatc aatcgccagc gtggtcatag caccgccttt ttcatcgctg tgttgtacca
    58621 ctgacaaatg tgttcaagac gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg
    58681 gcgcatcgca tctgccgcct gagcaggcgt gttgtaacgc ccttcggcaa tcaccctaca
    58741 tccggcgtcg ctcaacgttt tcaccagcgc cagatccggc tcttctggcg tttcaggcgt
    58801 ggtatagcca gaaagcgtag tgccaataat ttcggctccc agcttttggc atgccaggcc
    58861 gtcttccggc gttgagcagt cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag
    58921 cagcgtttca acaggcaccg gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc
    58981 gcccgcctgc gccagcgcat caacatcttc aatataggcc gtgatgcgta ccggagaatc
    59041 ctccagatcg cgtttcacaa ttccaataat cggcacgctc accaccgcac gcgtggcttg
    59101 cagatttgcc acaccttcaa tgcgaatggc aaccgcgccc gcctgttctg ccgctaatgc
    59161 catggcggcg acgatttcgg gtttatcgag cgggctgtcc ggaaccggct ggcaggagac
    59221 aatcaggcca ccgttagcag cgattttttg atccagttgt gcaagtaacg acatacatct
    59281 tcccttagcg aaaggcccgg tacatagacc gggcaacagg attaactttt ggttttgact
    59341 aaatcgtttt tggcgctgcc aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg
    59401 tcatgagtac gcaatgcttc cgggcgcagc caacgctgaa cgcgggaagg catatccaga
    59461 ccaatcagca ggatcaccac aaatgtcaga ctgaacgata gcgatgccag cgcagtaccc
    59521 agatccagac gttgagcgat caacgcgccg atgattgggg ccagtgcgcc gcccaatgca
    59581 ccaacgttgt aggtaaagcc caggcccgct gcacgctggt cggtatcgaa ataaccgcca
    59641 atcagtttcg gtaagatccc ggcgatccct tgtccaagca tttgctggaa gaacagtaac
    59701 agaccgagca cccagacgtt tgcgccgcca atcgcaaata ccggaataat caacagctgc
    59761 gaggccaaca ggctacaaac gtacgctttg cgggttccca gccagtcacc gaggaagccg
    59821 ccgacacagc accccaccgc agccccaaag ccgctaaaga acagcacatt ggcgacagta
    59881 tgcgggttat aagccagatc ggttttcaga tacgttggca gcagcgcctg aatcggccat
    59941 gagtagagga aagcaaacaa cacgaccacc atcagcatta cgcccgttgg ccagcgttta
    60001 cccgtacttt gcaccataaa gctgataaag attgcggcgc ataacagccc aagaacagcg
    60061 acgatcgcgg cattttgcag gttaccggcg aagcaaaacc acagcgcagt agccgccgcc
    60121 agtgtcatta cgatattggc aatgcgatgt tcaccacggt agagaatatc taccatcgtg
    60181 cgtactggtg ctttacctgc atgtttctct ttccagtctt ccgcttccgg gatgttttta
    60241 cgcagccaga gagcaaagat gataggcaaa atgccgataa agaacagcgc acgccagccc
    60301 cagaccggaa ccaccaggct atagacctga gcggcaacga cggcccccac agagaagcct
    60361 gaaatcagaa aaccactggc tttgttacgc agatgttttg gccagctttc aatgacatag
    60421 gtggcgctgg aaccgtattc acccgccatc cccatgccga tgaccagacg cgcgataaac
    60481 atggtgatgt agcctggcgc aaagccgcag gccagcgtcc cggccgagaa gagaacgatg
    60541 ctggtgacca ttgccagacg acgcccgtag cggtcaccca tagcgccgag catcaggccg
    60601 ccgaaccagc gagagataaa ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc
    60661 ccgaattcac cttgtacttc ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca
    60721 agcagatatc ccaaccaggc agcggaaaat gcccgccatt gtgcacgatt gagatggcga
    60781 taccacggga tattctgggt tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc
    60841 acacgctttg gtatgaaaat tgtagggtac agatgcgttt atttcccctc acccggtagg
    60901 ggcgagcgag gggaaaacaa ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt
    60961 cagttctggc agatattttt catctaccgg tccaaacggt ttgcggcaca gcggcacaga
    61021 aacgacatcc atataatgga ggacagtttt caggccgcgg aatacgcccg ttttgatcag
    61081 taaatcaatg actttattgc attcagtttg cagtttctgc gcggtctgga tatcgccttc
    61141 tttcagcgcc ttaacgatcc cctgatagcg ccagcccatg atgttgtagg tactaccgat
    61201 accaccatca gcgcccgcca gcagaccaga ggcgaagatt tcgtcgtaac cgttatagag
    61261 cacaagatca ggatgttcac gacggatctg ctccatctga tagagatcgc cagaagtctg
    61321 tttcagcgca ccgacgccag gcaacgtaac cagggtgttg atctgatcca gggtcagttt
    61381 taccccactc agggctggaa tgttgtacac caccatcggc aaaccatccg ctgaatcaat
    61441 aattgcccga tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga acggcgtgac
    61501 ggcggagacg gcatcgaagc cataacgttt agccgatgcc gcaagttgtt ggctttcggc
    61561 ggtgctgacg caaccgacgt gggcgatgag tttaatctta cctttcgcct cttcggcgac
    61621 gatttccagt acctgttcac gctcggaaag gctttgtaca aaggcctcgc cggtcgaacc
    61681 acccacgtat aaaccgtcga tgccctgctg aatattgaac tgaaccaggc gacgcagact
    61741 cgctttatcc agtgcttgtt gttgatcaaa aggagtcagg agtgcagcca ttacgccacg
    61801 taaattcgtt gccataaata cctctgaagt gatgcttgtc tgataaacga tataccttta
    61861 tacctgttat accagatcaa ttaagcaaca ccccatacag aaagcttata atgcgatctg
    61921 cttcactaaa gtggcattat ttctttttgt tggtggtctg accgaaagcg tgccaggtag
    61981 cagagacgct gttgagatgc gattgcaacg cacgatcggc ttcgtcagga tcatgacggc
    62041 ggatcgcatc aacgatcgca atatgctgtt gataactaac gttgttatgt tcgtgcagtg
    62101 cctgatcggt aaccgttggg cgtgcggcaa taagccagtc gagcagggca acgtggatcg
    62161 ccatgaagat tgggttaccg gggatctccg ccagcacgcg gtggaaatca acgtctgaac
    62221 gaatgaatgc cgcgttgtta tccagcgact gactgttaat ttccagtgct tttgccagca
    62281 aatcgatttg ctcatcggtg gcatgttcag ccgcatagcg caccaggctg gattcaaaga
    62341 acagacgtaa ttgttcgaaa tgggcaatcc caccaggatg agaaaggaaa tctttcgcca
    62401 tgccggaaag ctcgccgatg atggtgtccg cagaaggacg tgagacgcgg gcacgttcgc
    62461 cgttgttgat ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttcg cgcacagaag
    62521 gacgcccgac gttaaagaac gccatcagtt cgcgttcaga cggtaattgt tcaccttcgc
    62581 caaattcacg acggcggatc atctgttcca gctcttcttc caccatttcg gagagttttt
    62641 tacgcgccag cgggcggcta cgcaagttgc gaccaattgc aggtgaagaa tcttcggttt
    62701 gcgaatcaaa tgcgttcata aggcccattc tgtaaggtca gtgtgattaa catcatcagt
    62761 gacatcctat cacaggattg aaagtagggg aaaatggcag gtttttctct ttgtgcctca
    62821 tcattaccat aattaacgga ataattaact attgcgaaaa attaatgtaa cgcagataaa
    62881 aacatcccgt ttgaattatt tataa
//
LOCUS       DAZFJF010000012        62407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFJF010000012 DAZFJF010000000
VERSION     DAZFJF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.012"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(339..881)
                     /gene="ycfP"
                     /locus_tag="ACHKAU_000896"
     CDS             complement(339..881)
                     /gene="ycfP"
                     /locus_tag="ACHKAU_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO5530009.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(904..1929)
                     /gene="nagZ"
                     /locus_tag="ACHKAU_000897"
     CDS             complement(904..1929)
                     /gene="nagZ"
                     /locus_tag="ACHKAU_000897"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO5530010.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1940..2764)
                     /gene="thiK"
                     /locus_tag="ACHKAU_000898"
     CDS             complement(1940..2764)
                     /gene="thiK"
                     /locus_tag="ACHKAU_000898"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO5530011.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2745..3386)
                     /gene="lpoB"
                     /locus_tag="ACHKAU_000899"
     CDS             complement(2745..3386)
                     /gene="lpoB"
                     /locus_tag="ACHKAU_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO5530012.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3400..3777)
                     /gene="ycfL"
                     /locus_tag="ACHKAU_000900"
     CDS             complement(3400..3777)
                     /gene="ycfL"
                     /locus_tag="ACHKAU_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO5530013.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3780..4139)
                     /gene="hinT"
                     /locus_tag="ACHKAU_000901"
     CDS             complement(3780..4139)
                     /gene="hinT"
                     /locus_tag="ACHKAU_000901"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO5530014.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4473..6662
                     /gene="fhuE"
                     /locus_tag="ACHKAU_000902"
     CDS             4473..6662
                     /gene="fhuE"
                     /locus_tag="ACHKAU_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO5530015.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6722..8155)
                     /gene="ptsG"
                     /locus_tag="ACHKAU_000903"
     CDS             complement(6722..8155)
                     /gene="ptsG"
                     /locus_tag="ACHKAU_000903"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO5530016.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8450..9247)
                     /gene="ycfH"
                     /locus_tag="ACHKAU_000904"
     CDS             complement(8450..9247)
                     /gene="ycfH"
                     /locus_tag="ACHKAU_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5530017.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9258..10262)
                     /gene="holB"
                     /locus_tag="ACHKAU_000905"
     CDS             complement(9258..10262)
                     /gene="holB"
                     /locus_tag="ACHKAU_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO5530018.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10259..10900)
                     /gene="tmk"
                     /locus_tag="ACHKAU_000906"
     CDS             complement(10259..10900)
                     /gene="tmk"
                     /locus_tag="ACHKAU_000906"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5530019.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10890..11912)
                     /gene="yceG"
                     /locus_tag="ACHKAU_000907"
     CDS             complement(10890..11912)
                     /gene="yceG"
                     /locus_tag="ACHKAU_000907"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO5530020.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11915..12724)
                     /gene="pabC"
                     /locus_tag="ACHKAU_000908"
     CDS             complement(11915..12724)
                     /gene="pabC"
                     /locus_tag="ACHKAU_000908"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO5530021.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12844..14085)
                     /gene="fabF"
                     /locus_tag="ACHKAU_000909"
     CDS             complement(12844..14085)
                     /gene="fabF"
                     /locus_tag="ACHKAU_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO5530022.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14173..14409)
                     /gene="acpP"
                     /locus_tag="ACHKAU_000910"
     CDS             complement(14173..14409)
                     /gene="acpP"
                     /locus_tag="ACHKAU_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5530023.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14620..15354)
                     /gene="fabG"
                     /locus_tag="ACHKAU_000911"
     CDS             complement(14620..15354)
                     /gene="fabG"
                     /locus_tag="ACHKAU_000911"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO5530024.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15367..16296)
                     /gene="fabD"
                     /locus_tag="ACHKAU_000912"
     CDS             complement(15367..16296)
                     /gene="fabD"
                     /locus_tag="ACHKAU_000912"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5530025.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16312..17265)
                     /gene="fabH"
                     /locus_tag="ACHKAU_000913"
     CDS             complement(16312..17265)
                     /gene="fabH"
                     /locus_tag="ACHKAU_000913"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO5530026.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17333..18403)
                     /gene="plsX"
                     /locus_tag="ACHKAU_000914"
     CDS             complement(17333..18403)
                     /gene="plsX"
                     /locus_tag="ACHKAU_000914"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO5530027.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18484..18657)
                     /gene="rpmF"
                     /locus_tag="ACHKAU_000915"
     CDS             complement(18484..18657)
                     /gene="rpmF"
                     /locus_tag="ACHKAU_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO5530028.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18709..19230)
                     /gene="yceD"
                     /locus_tag="ACHKAU_000916"
     CDS             complement(18709..19230)
                     /gene="yceD"
                     /locus_tag="ACHKAU_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO5530029.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19429..20013
                     /gene="yceF"
                     /locus_tag="ACHKAU_000917"
     CDS             19429..20013
                     /gene="yceF"
                     /locus_tag="ACHKAU_000917"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO5530030.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20125..21084)
                     /gene="rluC"
                     /locus_tag="ACHKAU_000918"
     CDS             complement(20125..21084)
                     /gene="rluC"
                     /locus_tag="ACHKAU_000918"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO5530031.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21657..24842
                     /gene="rne"
                     /locus_tag="ACHKAU_000919"
     CDS             21657..24842
                     /gene="rne"
                     /locus_tag="ACHKAU_000919"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO5530032.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25038..25991)
                     /gene="flgL"
                     /locus_tag="ACHKAU_000920"
     CDS             complement(25038..25991)
                     /gene="flgL"
                     /locus_tag="ACHKAU_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5530033.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26003..27646)
                     /gene="flgK"
                     /locus_tag="ACHKAU_000921"
     CDS             complement(26003..27646)
                     /gene="flgK"
                     /locus_tag="ACHKAU_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5530034.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27712..28653)
                     /gene="flgJ"
                     /locus_tag="ACHKAU_000922"
     CDS             complement(27712..28653)
                     /gene="flgJ"
                     /locus_tag="ACHKAU_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO5530035.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28653..29750)
                     /gene="flgI"
                     /locus_tag="ACHKAU_000923"
     CDS             complement(28653..29750)
                     /gene="flgI"
                     /locus_tag="ACHKAU_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5530036.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29762..30460)
                     /gene="flgH"
                     /locus_tag="ACHKAU_000924"
     CDS             complement(29762..30460)
                     /gene="flgH"
                     /locus_tag="ACHKAU_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5530037.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30513..31295)
                     /gene="flgG"
                     /locus_tag="ACHKAU_000925"
     CDS             complement(30513..31295)
                     /gene="flgG"
                     /locus_tag="ACHKAU_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5530038.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31433..32188)
                     /gene="flgF"
                     /locus_tag="ACHKAU_000926"
     CDS             complement(31433..32188)
                     /gene="flgF"
                     /locus_tag="ACHKAU_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO5530039.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32208..33413)
                     /gene="flgE"
                     /locus_tag="ACHKAU_000927"
     CDS             complement(32208..33413)
                     /gene="flgE"
                     /locus_tag="ACHKAU_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5530040.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33438..34133)
                     /gene="flgD"
                     /locus_tag="ACHKAU_000928"
     CDS             complement(33438..34133)
                     /gene="flgD"
                     /locus_tag="ACHKAU_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO5530041.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34145..34549)
                     /gene="flgC"
                     /locus_tag="ACHKAU_000929"
     CDS             complement(34145..34549)
                     /gene="flgC"
                     /locus_tag="ACHKAU_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5530042.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34553..34969)
                     /gene="flgB"
                     /locus_tag="ACHKAU_000930"
     CDS             complement(34553..34969)
                     /gene="flgB"
                     /locus_tag="ACHKAU_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO5530043.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35124..35783
                     /gene="flgA"
                     /locus_tag="ACHKAU_000931"
     CDS             35124..35783
                     /gene="flgA"
                     /locus_tag="ACHKAU_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5530044.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35859..36152
                     /gene="flgM"
                     /locus_tag="ACHKAU_000932"
     CDS             35859..36152
                     /gene="flgM"
                     /locus_tag="ACHKAU_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5530045.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36157..36573
                     /gene="flgN"
                     /locus_tag="ACHKAU_000933"
     CDS             36157..36573
                     /gene="flgN"
                     /locus_tag="ACHKAU_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO5530046.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36613..38148)
                     /gene="murJ"
                     /locus_tag="ACHKAU_000934"
     CDS             complement(36613..38148)
                     /gene="murJ"
                     /locus_tag="ACHKAU_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO5530047.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYIFLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38258..39181)
                     /gene="yceM"
                     /locus_tag="ACHKAU_000935"
     CDS             complement(38258..39181)
                     /gene="yceM"
                     /locus_tag="ACHKAU_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5530048.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTVLWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39183..39830)
                     /gene="yceH"
                     /locus_tag="ACHKAU_000936"
     CDS             complement(39183..39830)
                     /gene="yceH"
                     /locus_tag="ACHKAU_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO5530049.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39841..40425)
                     /gene="rimJ"
                     /locus_tag="ACHKAU_000937"
     CDS             complement(39841..40425)
                     /gene="rimJ"
                     /locus_tag="ACHKAU_000937"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5530050.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40661..41869
                     /gene="mdtH"
                     /locus_tag="ACHKAU_000938"
     CDS             40661..41869
                     /gene="mdtH"
                     /locus_tag="ACHKAU_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO5530051.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41933..42580
                     /gene="grxB"
                     /locus_tag="ACHKAU_000939"
     CDS             41933..42580
                     /gene="grxB"
                     /locus_tag="ACHKAU_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO5530052.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42714..43274
                     /gene="yceB"
                     /locus_tag="ACHKAU_000940"
     CDS             42714..43274
                     /gene="yceB"
                     /locus_tag="ACHKAU_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5530053.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43380..44426
                     /gene="pyrC"
                     /locus_tag="ACHKAU_000941"
     CDS             43380..44426
                     /gene="pyrC"
                     /locus_tag="ACHKAU_000941"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO5530054.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVHWSVKQ"
     gene            44500..44745
                     /gene="dinI"
                     /locus_tag="ACHKAU_000942"
     CDS             44500..44745
                     /gene="dinI"
                     /locus_tag="ACHKAU_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO5530055.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            45035..45289
                     /gene="bssS"
                     /locus_tag="ACHKAU_000943"
     CDS             45035..45289
                     /gene="bssS"
                     /locus_tag="ACHKAU_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO5530056.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45404..46522
                     /gene="solA"
                     /locus_tag="ACHKAU_000944"
     CDS             45404..46522
                     /gene="solA"
                     /locus_tag="ACHKAU_000944"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO5530057.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            46570..46683
                     /locus_tag="ACHKAU_000945"
     CDS             46570..46683
                     /locus_tag="ACHKAU_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO5530058.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            46944..47510
                     /gene="yceJ"
                     /locus_tag="ACHKAU_000946"
     CDS             46944..47510
                     /gene="yceJ"
                     /locus_tag="ACHKAU_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5530059.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            47514..48089
                     /gene="yceI"
                     /locus_tag="ACHKAU_000947"
     CDS             47514..48089
                     /gene="yceI"
                     /locus_tag="ACHKAU_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO5530060.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(48131..49183)
                     /gene="trhO"
                     /locus_tag="ACHKAU_000948"
     CDS             complement(48131..49183)
                     /gene="trhO"
                     /locus_tag="ACHKAU_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO5530061.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            49408..50328
                     /gene="lpxL"
                     /locus_tag="ACHKAU_000949"
     CDS             49408..50328
                     /gene="lpxL"
                     /locus_tag="ACHKAU_000949"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO5530062.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            50500..51726
                     /gene="mdtG"
                     /locus_tag="ACHKAU_000950"
     CDS             50500..51726
                     /gene="mdtG"
                     /locus_tag="ACHKAU_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO5530063.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            51809..52183
                     /gene="msyB"
                     /locus_tag="ACHKAU_000951"
     CDS             51809..52183
                     /gene="msyB"
                     /locus_tag="ACHKAU_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5530064.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(52184..52411)
                     /gene="yceK"
                     /locus_tag="ACHKAU_000952"
     CDS             complement(52184..52411)
                     /gene="yceK"
                     /locus_tag="ACHKAU_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5530065.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(52584..55127)
                     /gene="mdoH"
                     /locus_tag="ACHKAU_000953"
     CDS             complement(52584..55127)
                     /gene="mdoH"
                     /locus_tag="ACHKAU_000953"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO5530066.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(55120..56655)
                     /gene="mdoG"
                     /locus_tag="ACHKAU_000954"
     CDS             complement(55120..56655)
                     /gene="mdoG"
                     /locus_tag="ACHKAU_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO5530067.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            57049..58206
                     /gene="mdoC"
                     /locus_tag="ACHKAU_000955"
     CDS             57049..58206
                     /gene="mdoC"
                     /locus_tag="ACHKAU_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO5530068.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(58214..59695)
                     /gene="clsC"
                     /locus_tag="ACHKAU_000956"
     CDS             complement(58214..59695)
                     /gene="clsC"
                     /locus_tag="ACHKAU_000956"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO5530069.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(59637..60170)
                     /gene="ymdB"
                     /locus_tag="ACHKAU_000957"
     CDS             complement(59637..60170)
                     /gene="ymdB"
                     /locus_tag="ACHKAU_000957"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO5530070.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(60265..60576)
                     /gene="ymdA"
                     /locus_tag="ACHKAU_000958"
     CDS             complement(60265..60576)
                     /gene="ymdA"
                     /locus_tag="ACHKAU_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO5530071.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(60697..61029)
                     /gene="csgC"
                     /locus_tag="ACHKAU_000959"
     CDS             complement(60697..61029)
                     /gene="csgC"
                     /locus_tag="ACHKAU_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO5530072.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(61088..61543)
                     /gene="csgA"
                     /locus_tag="ACHKAU_000960"
     CDS             complement(61088..61543)
                     /gene="csgA"
                     /locus_tag="ACHKAU_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO5530073.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(61584..62039)
                     /gene="csgB"
                     /locus_tag="ACHKAU_000961"
     CDS             complement(61584..62039)
                     /gene="csgB"
                     /locus_tag="ACHKAU_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO5530074.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(62168..>62407)
                     /locus_tag="ACHKAU_000962"
     CDS             complement(62168..>62407)
                     /locus_tag="ACHKAU_000962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530075.1"
                     /translation="FSAPPWTIFYCKKTRFVTACAQDISQQSATGKKNDCLVIFDSGK
                     RRFKRKQNIFCVDNSVFTWVLILWYEQKKKNTTRG"
BASE COUNT        15244 a        16597 c        15317 g        15249 t
ORIGIN      
        1 taacaacagg ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca
       61 ttggtcatac caaaattgat gttaatcaag ttttgttgct gaaaagatag gcgactttac
      121 cgggtgagat caaaaaacag agtttattgt ttattatttg agcaagtccg gaagggcgat
      181 atagggtgaa gcgtatatcg agggcgtttg gtcgtcctga taagaggcgc atcaggcatt
      241 gatgctcgaa tgtcggatgc ggcgtgaacg ccttatccga cccacagaat ccggagcgca
      301 tcaggtaccg gcaaggttac ctgatgcgac aagggcattt acccgagggt tttgaacgct
      361 ttaatgcgct gtaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag
      421 acaatctcgt aataatgatg cagctcttca gatgtccgct ggctgttaag cgcttcatca
      481 ttacgcgaca aaatcaccag gcaacgatcg cgattcttct cacggaagtt ggtcacacac
      541 ttagtggcaa tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa
      601 gggaacaaat tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc
      661 gcccagtatc cgcccagacc aacgccgcaa atcagcggac gctcgtcgac gttcagttgc
      721 aacattttgt ccacttcttt gagcagatgc tgcatatcat gtttcggatg ccgcgtactg
      781 tagcttatca agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta
      841 cccggactgt tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca
      901 gggttagtga cctgctttct cttcctgcca gcgttcatgt aactgattca gacgggtgct
      961 gatcgctttc cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata
     1021 caaacgtgta acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc
     1081 tttacgatta ttgcagacca ggatcatatc gcaacccgca tccagtgatg cctgcccgcg
     1141 ttcggcataa ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac
     1201 gccgtcaaaa cccagttcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc
     1261 cggacgcgga tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt
     1321 ttcgcggatt aaggaactga agaccgacat atctttagcg cgaatctccg cttgtggacg
     1381 tggatcgcac ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg
     1441 tttcccggtc gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc
     1501 cagggctttt tgtggatcgg catgataaga acgctcgcca atcgccgcgc tgatatgccc
     1561 gacatccagc actggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat
     1621 cagccaaccc gcctcttgcg ccagtttgcc gccctcttcc attcctgaca gcgcagcgaa
     1681 tgattgcgcc gctggcaagc gggtaaaacc ttcacgaaaa cgctgcacgc gtccaccttc
     1741 ctgatctacc gccaccacca gatgattgcg cgaagctgcg cggatctggc gcaccagttc
     1801 acgcaactgg gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg
     1861 atgcgcaagt atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaacattac
     1921 tggacccaca ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca
     1981 gcctgataaa ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga
     2041 gcatcagcag ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct
     2101 tcgcgcgagt ggcatagtca ttgaccaatt gccggtgctg ttcagtattt tccacccaca
     2161 ctgccgccag ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta
     2221 accctgacgc gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc
     2281 gtaaaggccg tggttcccgc gctttgcgca gacgttttaa cattcgcagc caacccactg
     2341 tccgccgcgc cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc
     2401 gccagccaaa acgtggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat
     2461 ccggcaaata cgtttttacc tcgccgggca gatagtcgac taccatccag tcacggagat
     2521 ataaatgcgg cttcggtgca atgcttgcgg gtagttgtga taaagcccga tactggcgca
     2581 agaacgcgga ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct
     2641 gatgttcaat gagaaaactc ccgccactaa gcccactatt aaacgtcgtg acgggatgaa
     2701 actgcgggaa aaagcgcgac agcaattcgt cgcgcatgat gggattattg ctgcgaaacg
     2761 gcacctttac ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg
     2821 gtcggagcgt taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg
     2881 cgggcaatgc ctatggcttt actacgggta cctaaactgt cctgcggcga caaacctaac
     2941 tgttgcttag ccatcgacag ctgctgggcg gaaaccaggg taaatttccc gttattagcc
     3001 agcgcatttc gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg
     3061 ttaacgctat caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta
     3121 ctgaccatcg gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt
     3181 gccgtttgat cttcgtgctc aattgggcct ggctgctgcg ggatcgtcgg caccgagggc
     3241 actgtgggga caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct
     3301 accggtgcag gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc
     3361 aaggcgtagc gactcatttt tgtcatcaag attcacccct tacaaatata gataaagtct
     3421 gactttgtgc gcccccagaa aattggcgct gccgtacagc gttaccgccg aatgtgcggg
     3481 aatggtaacg ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca
     3541 gtaaaaacga taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc
     3601 tgaaggttga atatcagacg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa
     3661 taaagatgac tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg
     3721 acagcccacc agcagtaaca acgccagaga caccagccca aagcatcctt ttctcatcgt
     3781 tacagacctt tatgtgccag cattggtccc agcggacggc cacccaacaa gtgcatatgg
     3841 atgtggtaaa cctcttgtcc gccatggcgg ttggtattca tgatcagacg atagccatct
     3901 tcggcaatac cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc
     3961 tgctcatgct cagctgacac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga
     4021 atatgcgttg gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag
     4081 acgatatcgg aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg
     4141 acattttcct ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag
     4201 tatagagtat ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa
     4261 aaaatcgttt tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg
     4321 aaaagacatt ctctgtttgg tttacacgtc aaatatggat gcgtatattt ctcatttgca
     4381 tttacataca aaattattcg cacactaaat ataaccgctc atcaccctcc tccggcatga
     4441 gcctgacgac aacataaacc aagagatttc aaatgctttc aacacaattt aacagggata
     4501 atcaatatca agccagcacc aaaccatcac tacttgccgg ttgcatagca ttggcactat
     4561 taccttctgc cgcttttgcc gctccagcca ctgaagaaac ggtgattgtt gagggttcag
     4621 ccacagctcc agatgatggc gaaaatgatt acagcgtaac gtctacctct gcgggtacca
     4681 aaatgcagat gactcaacgt gatattcctc agtcggtcac tattgttagc cagcagcgga
     4741 tggaagatca gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca
     4801 aaagtcaggc ggattccgat cgtgctcttt attattcccg cggattccag atcgataact
     4861 atatggttga tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt
     4921 ctgatatggc actctttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg
     4981 ggacgggtaa tccatctgcg gcaattaata tggttcgaaa acacgcgacc agtcgtgaat
     5041 ttaaaggcga tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt
     5101 tacaaagccc actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga
     5161 ataacgactc atggctggac cgctacaaca gtgaaaagac cttcttctcc ggcattgtcg
     5221 atgctgattt aggcgatctt acgacgcttt cagccggtta cgaatatcag cacattgatg
     5281 ttaatagccc aacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt
     5341 acgatcgcgc acgcagtacc gcacctgact gggcgtacaa cgataaagag atcaacaagg
     5401 tctttatgac cctgaagcag cggtttgctg atacctggca agcgacgctg aatgccaccc
     5461 actctgaagt cgaatttgac agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg
     5521 atggtatgct gattgggcca tacagtaatt atggacctgg ctttgattat gtcggcggca
     5581 ccggttggaa cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct gacggtagtt
     5641 atgaattgtt tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca
     5701 atcgttactt cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact
     5761 ttaatggcaa tttcccacaa accgactggt caccacagag cctggcgcag gacgatacca
     5821 cacatattaa atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga
     5881 tcctcggcgc acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa
     5941 accacaccac gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg
     6001 ccagctatac ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg
     6061 ctccaatcac cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc
     6121 tgaccaccac gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta
     6181 cacctatccc cggcagcaac ggcgaaaccg cctataaagc agtggatggg acagtcagta
     6241 aaggggtgga atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg
     6301 caacgcgcta tattgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca
     6361 ccacggttaa aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg
     6421 gtggtgttaa ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc
     6481 gcgccgagca aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa
     6541 acttctcgtt acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacgtgg
     6601 aaggttctat cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct
     6661 gaatagcacc tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacag
     6721 attagtggtt acggatgtac tcatccatct cggttttcag gttgtcggat ttagtaccga
     6781 aaatcgcctg aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc
     6841 cagcctgatc cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt
     6901 cgaggttagt aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc
     6961 tggtacctgt cgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca
     7021 gtgctttaat cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga
     7081 tcgggaacag ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt
     7141 gcgagaacga cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg
     7201 ccagaatcgc gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg
     7261 gctcggtgat accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt
     7321 tcgcgcggtt ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt
     7381 acattttgaa caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac
     7441 gcggaatgtc gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct
     7501 ggaaaggtac gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac
     7561 cgtaaatgcc aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga
     7621 ttgcagaacc aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca
     7681 ggccagaaat gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct
     7741 taatacggta gaaacggtta aacatgtacg ctgcgatcgc accggagata atccctccga
     7801 gtacgccagt atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca
     7861 gtggcgcaac cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg
     7921 cggatacgcc atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca
     7981 tgtttgcaaa gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc
     8041 agctgaaatt cgcggaaccg acgcccagca gaatacctgc gataggcagt acggataccg
     8101 gcagcatcag cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg
     8161 agagtgctcc tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc
     8221 ctcgccgtgt acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag
     8281 tgccaatttg tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt
     8341 ttgacaaaac atatgtcaca atatttcaaa cgtggttatt caccgcccaa ttttcggcgg
     8401 tgaactttac tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg
     8461 attgaaggcg ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg
     8521 ccagttcttc aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca
     8581 tcgcaggttg attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt
     8641 ccaccagtaa tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac
     8701 ggaaggtcac aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg
     8761 tttctctgtc ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc
     8821 gcagaattgc cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca
     8881 gttcacgacc aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg
     8941 gtgtgtaata ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca
     9001 gacggcgtaa atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag
     9061 aaaatacgac gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg
     9121 ttgtggcgac tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat
     9181 ccacgtcctt atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta
     9241 aaaacatgat gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa
     9301 tgctcaatac gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtaacagac
     9361 attaactgtt cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa
     9421 agatggtttg ccagttcggc gaccaggccc ggcacatcaa cattggtcac ctgcgcagca
     9481 ccatgatggc gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga
     9541 gcttgttcat gattaagggc cgctagcagc gaataccagt cgcccgatgg cacgctatat
     9601 gccaacgcct gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt
     9661 gccgcgccag gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt
     9721 gtcacttcgc gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc aaggtaatgt
     9781 aaccgacaac gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa
     9841 aaccaggttt ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg
     9901 gtcagtaagg cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca
     9961 ttcagctttt cggtgacctc acgtaccgca tcaatgccca gcatattttt ccctttttcg
    10021 ggagccaggg tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag
    10081 tgaccgcaac ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag
    10141 attaaagcat catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt
    10201 ccggcctgat agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc
    10261 atgcgtccaa ctccttaacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct
    10321 ccagcggctg ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat
    10381 agcgggcgcg ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc
    10441 cgcgcgcacg cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt
    10501 cggggcgaaa atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac
    10561 cacgtcctcc gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccagg
    10621 tgccgttagc cagagctggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa
    10681 acatcagaac ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca
    10741 ccaggcttct taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt
    10801 cgcggatacc cagttgctca agcgtctcaa ccaccacatt acgcgcggta gttttgcctg
    10861 cgccttccag cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc
    10921 agataatcct gcacagactt gttatggctg gcaagattgg tattaaacgt gtgaccacct
    10981 ttaccatcgg ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc
    11041 gaatccgccc ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta
    11101 tacgctgtcg gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc
    11161 ccgtaaatca cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat
    11221 accgaggcaa cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc
    11281 atcgtcacca gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg
    11341 ctatcgaccg ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac atcggtggta
    11401 ttggcggtat acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc
    11461 agttcaagtg cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac
    11521 ggagcctcac gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc
    11581 gggaactgtg cttctttacc gctttccaac aatttcagca tctcgcgcac ggtcatctgc
    11641 ggtgtaaagc ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc
    11701 cattgaaaaa cccgtgggcg attgatgatc ttatcagcat aaagctgttc accgagcgcc
    11761 agacgtccgg tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg
    11821 tcggcaagat ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc
    11881 aataacaaga taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg
    11941 ctaaatattc atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca
    12001 tcactggcat taacgcatta caaataacca tctcatctgc ctgcaacgcc tcttccagag
    12061 aggcttgcac ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc
    12121 gcataatgcc gttaacacct gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc
    12181 gccagaacaa attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg
    12241 cctcatcagc gtttgtctgc tcaagatgag agcggatcaa tacttgctca agccgattaa
    12301 gatgtttaat acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg
    12361 tcatcccttc gttacgcaaa cggtcgtaat gtgcaggata aaccgtaacg gagagaatcc
    12421 gcgttgccgg tccactgttc aatgtgctgt accctcgccc gccactaccg cgactgatca
    12481 cgactttcag cacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct
    12541 gaggccagaa gtcacagaaa atcatcaacc gctgacaagc atcctgtagt cgctggatat
    12601 gcgccgataa caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac
    12661 caaactgcgt tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga
    12721 acataccggc tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt
    12781 cggaataaaa gctaagaaaa aaggcccgca agcggacctt ttatatgggt ggaaaatgac
    12841 aacttagatc tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca
    12901 cagcgtgtat tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca
    12961 accttcatcc gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag
    13021 gatggagtag atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt
    13081 cgtggagctt accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc
    13141 ttcagcttta tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact
    13201 cgcttcaatg cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt
    13261 ttctggcggt gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc
    13321 gtaaattttc gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc
    13381 accatcgccc agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg
    13441 gttatcattg cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt
    13501 actggctttc tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg
    13561 cgcagcatga ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg
    13621 gccacgcagg ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg
    13681 aacgaagaat gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc
    13741 gatcagtccg aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc
    13801 ttccgttatt tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg
    13861 aatgaaggca tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc
    13921 ctttactaag ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct
    13981 gatgccactc tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg
    14041 agacaacatg cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg
    14101 agggaaaaaa tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct
    14161 ggagatgttc acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt
    14221 gattttctca gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac
    14281 cagctcaacg gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt
    14341 aacttcttcc tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat
    14401 agtgctcata ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta
    14461 taaaatgttg aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt
    14521 ttgaggcctt accccccaat aacgcaaatc attttgcact aattattact cataaccacg
    14581 cagactgcgc gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc
    14641 gttcacatgc aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac
    14701 cgcgttggcg atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc
    14761 cgcacgctgg tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac
    14821 aacgtttaca gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat
    14881 caagcccgct ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac
    14941 agaaccgata gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt
    15001 tgacagacgg aaaacagatg aaaggttggt ttcgataatt tcgttccact cttcatcttt
    15061 cattcgcatt aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc
    15121 accaaattct gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt
    15181 caacatcaga cctttgccgt tggcacctaa ataatcgctg atcgcctgag cgccattttc
    15241 actggtcgca gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg
    15301 gccaattccg cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt
    15361 cctcttttaa agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt
    15421 cagggtgtcg acaatgcgtt tcgtcaggcc agtaagcacc ttgcccgggc cgacttcata
    15481 gagatgttct acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt
    15541 atacaactga cgtaccagtg cgtcacggat ggcatcacca ttggtttcgc atttcacatc
    15601 aacgttattc acaacagaaa ctgttggtgc gttaaaggtg attttcgcta attctactgc
    15661 cagtttgtcg gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg
    15721 cagcgcgcgt tttgcgccag cagctttaca ggcagcgcca gcacgctcaa ccgcttcttt
    15781 atgaccggca ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc
    15841 ttctgcagct tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc
    15901 catagcgcca gtgccttccg gtacggcttc ttgcatgaac ttaccgcgca tctcaaccag
    15961 acgcaccgca tcagcgaaat taatcacgcc agcgcaaacc agggcggagt attcccccag
    16021 gctgtgaccg gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag
    16081 cgcaacagat gcaatcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc
    16141 tggcccctgc tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa
    16201 cgtttcttcg acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga
    16261 accctgtcca gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg
    16321 aaccagcgcg gagccccagg tgaatccacc gccaaaggct tcaagcagaa ccaactgccc
    16381 cggcttaatg cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga
    16441 ggtattaccg tggcgatcca gcgtcaccac gacattgtcc atagacatac cgagtttttt
    16501 cgccgttgca ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga
    16561 acggtcaaga ttatttgccg ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc
    16621 aaccttgaag acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacacg
    16681 gtcagcatta ggcagcgtca gcaactcacc atagctaccg tcggcatgca gatgggtgga
    16741 gatgattccc ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat
    16801 aataatagtc ccacgatcgg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac
    16861 cagagcatac ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt
    16921 gaaacctgcg caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccaacatgct
    16981 ttgaatctga caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat
    17041 caggccaatc tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa
    17101 gcccatggtt gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg
    17161 agtgacaatc cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac
    17221 ttgttcgggc agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact
    17281 tttcggttat ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca
    17341 gagttccgct tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc
    17401 gagcggcaat tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga
    17461 ccgcaaaagc tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta
    17521 acagacaggc gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct
    17581 tttgtagcca acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag
    17641 atttcagcag agaaaggaac atcctgacaa caccttccat cgtctttaac gtgacatttc
    17701 ctgtaaagcc gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa
    17761 gatagccgat ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac
    17821 tgtcgagacc ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa
    17881 tttccaccac ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtgc
    17941 tgtcacaatc gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg
    18001 gtaataccgt caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg
    18061 ccagccccat cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt
    18121 tcaccagctc cagggccata cgcattgaac tcccacgact ggcgcggata gcttgcgaag
    18181 gccgggcatc actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt
    18241 caaagtcagc tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag
    18301 tgagttgcga attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa
    18361 aatcccctcc catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg
    18421 gtttggtctt tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg
    18481 tgattactta gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg
    18541 caggtgtttt tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc
    18601 atgggaacga cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga
    18661 ccttactcct caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt
    18721 taagctggct aatacggcaa atgggtttgg cttttgcgct tcttcaggca gttcaccaaa
    18781 gaccatgtcc gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa
    18841 ggcgaggatg atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac
    18901 ctcaatcggt tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact
    18961 aaaacaatac gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag
    19021 cgttaccgtc accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa
    19081 cgacatggag cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac
    19141 ctgatcaggg gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc
    19201 gagagtcagg ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat
    19261 agagtcaaag aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg
    19321 gttactgctt tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca
    19381 tagtttaaaa tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt
    19441 attttagcct ccacctcgcc atggcgtcgc gctcttctgg aaaaactgca aatctctttt
    19501 gaatgtgcag caccagaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg
    19561 gtgcttcgac tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta
    19621 attattggtt cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg
    19681 gaagaaaatg cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact
    19741 ggactggcgt tgtttaattc ggcgaatggg catctacaaa cagaagtgga gccttttgac
    19801 gtccatttcc gtcacctgag cgaggcggag attgataatt acgtgcgtaa ggagcatccc
    19861 ctgcactgcg cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta
    19921 gagggacgtg accctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta
    19981 cgtcgggaag ggaaaaaccc gctgatggga tagttgtttg cgccggatgc ggttacgtcg
    20041 catccggcaa tcggtgcagg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc
    20101 cattatcagg cttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt
    20161 caaaccttca tccatcggcg cttcgatacg catcacctca ccggtccccg gatgggtaaa
    20221 cttcaacgct gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg
    20281 tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatactg
    20341 tgtatgcaca cggatctgat gagtacgtcc ggttaccggg ctacaacgca ccagggtggc
    20401 aaatgcatag cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactgac
    20461 acgcacgata cgttcgccgc tttgcagaat atttttcaat aacggcgctt gaacgctctt
    20521 cacatgcgac tgccactgac cgcgcaccag cgccaggtaa tctttttgca ttcctttttc
    20581 acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc
    20641 tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc
    20701 ccgcaaacct tcgataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga
    20761 aggtttattc agcaccagga tatgatcatc ttcatataag atgacgtccg ccagcgccgc
    20821 caccttttgc agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg
    20881 gatacgcacc tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac
    20941 ccgcacttca cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg
    21001 ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt
    21061 tacggatgga gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc
    21121 actcattttt ctatgcttat atttagtttt acaccttatt acttcactac gtgatcactt
    21181 tattgatggt tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg
    21241 ataaatggta aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc
    21301 cgtgtccatc cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc
    21361 atccacgcag caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt
    21421 gcagtcctta ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga
    21481 taattgcgct gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct
    21541 gacgataacc gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa
    21601 aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga
    21661 aaagaatgtt aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc
    21721 agcgtctgta tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct
    21781 acaaaggtaa aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg
    21841 ctgaacgtca cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact
    21901 acagtgctca tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg
    21961 ttcagatcga taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc
    22021 tggcgggtag ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc
    22081 gtatcgaagg cgacgaccgt accgaattaa aagaagcact ggcgagcctt gaactgccgg
    22141 aaggcatggg gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcgctgcaat
    22201 gggatttaag cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc
    22261 cggctccgtt cctgattcat caggagagca acgtaatcgt tcgcgcattc cgcgattact
    22321 tacgtcagga catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc
    22381 agcatatcgc tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg
    22441 agatcccgct gttcagccac taccagatcg agtcgcagat cgaatccgcc ttccagcgtg
    22501 aagtccgtct gccgtccggt ggttccattg ttatcgacag caccgaagcg ttaaccgcca
    22561 tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat cgaagaaact gcgtttaaca
    22621 ccaacctcga agctgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc
    22681 tgattgttat cgacttcatc gacatgacgc ctgtacgcca ccagcgtgcg gtagaaaacc
    22741 gtctgcgtga agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct
    22801 ttggcctgct ggaaatgtcc cgtcagcgcc tgagcccatc actgggtgaa tccagtcatc
    22861 acgtttgtcc gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct
    22921 ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca
    22981 ttgttcctgt gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtaaatgcca
    23041 ttgaaactcg tcaggacggt gtgcgctgtg taattgtgcc aaacgatcag atggaaaccc
    23101 cgcactacca cgtgctgcgc gtgcgtaaag gggaagaaac cccaacctta agctacatgc
    23161 tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta
    23221 agcgtccgga acaacctgcg ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa
    23281 cgccagctga acctgccgcg cctgttgtag ccccagcacc gaaagctgca ccggcaacac
    23341 cagcaactcc tgcacaacct gggctgttga gccgcttctt cggcgcactg aaagcgctgt
    23401 tcagcggtag tgaagaaacc aaaccgaccg agcaaccagc accgaaagca gaagcgaaac
    23461 cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc
    23521 gccgcgacac ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta
    23581 atcgtcgcca ggcacagcag cagactgccg agacgcgtga gagccgtcag caagctgagg
    23641 taacggaaaa agcacgtacc accgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc
    23701 gccgtaacga tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc
    23761 aatctgttca ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac
    23821 agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg
    23881 caccggtggt tgaagaaact gccgctgccg aaccaattgt tcaggaagct ccagctccac
    23941 gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag
    24001 aagagaacaa tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc
    24061 ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa
    24121 cccagtcgcc aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag
    24181 tctggatccg ctatccaatt gtacgtccgc aagatgtaca agttgaagag cagcgcgaac
    24241 aggaagaagt acaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac
    24301 ctgttgttag cgcgccagtt gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg
    24361 ttgccgaacc gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg
    24421 taactgaaca gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagtcgcag
    24481 aacacgcaga accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgccg
    24541 aaactgctga ggttgtggtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtcg
    24601 tcgctgaagt cgcagcagaa gttgaaacgg tagccgcggt tgaaccagag atcaccgttg
    24661 agcataacca cgctaccgcg ccaatgacgc gcgctccggc accggaatat gttccggagg
    24721 caccgcgtca cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag
    24781 gtggtcatac ggcaacgcat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt
    24841 aataattagc tcaaagtaat caagccctgg taactgccag ggctttttta tttcatcttt
    24901 gaatcaacat attaaaattc atcaaatggc atccttgcta accaacaatg caaaataggc
    24961 aaaaaaatgc ccgccccggc gatcccagag caggcagaca aaacataccc agtttcatga
    25021 tatgttttaa agcgagctta tttgttgagc tggaagagcg acaatccctg catatcggta
    25081 aatgctttat acgatgcctg caatgccgtt tgctgcatga tgtaagatga aatagttgca
    25141 ttccagtcca catcaaccag atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta
    25201 cctaatgaat ccagcgactc cagttcgttc agctgcgtgc ctaattccgc gcgaactgta
    25261 aggacattat tcagcgagtt tttcagtcca cggttggttt tatccaatgc cgcagcggcg
    25321 atttctttat ccgcttcgct atccgcgacc ggcgttttca gggctgcgat ggcgctatcc
    25381 agcatggcaa aaagattggt ttcagaagcg ctaccgtctg gttccgctac cgcgttactg
    25441 gtaatactgt cgaaaatttt gtcgcccgta tgtcctatca ccatcgaacg cgaagcatcg
    25501 acctgttgcc taatactttc tgctccaccg acgtatttcc ctttctcttc gctaaacggc
    25561 gcagtctctg ttttataacc ggcaaaaatg tagcgcccgt taccgtcagt ggtgttcgcc
    25621 agattcagca actggtcacg aagcccctgg atatccgtag ccagcgaggc ccggtcatcg
    25681 tcactcaaag tgccattgct ggcgtagaca attttttcct gagcattctg gatagcagtg
    25741 gtgacctggc taagtacact ctcttccagt gacacttttt gagtggcgaa agtacgcgcc
    25801 agcgtgtact ggctgttttg cgcctgtgcc tgggagagaa caaccgcttg tgatgctgca
    25861 atgggatcgt cagaagggtt aacgactcgc ttacctgtcg acatctgctc gccgtacttc
    25921 atccattccg cctgagaatt ggtgatgcca cgcatgtttt gctggtacat catctgggta
    25981 ctgaaacgca tcttatctcc ccttagcgaa tgttaatcag cgcatcaaaa atcgcgtttg
    26041 ccgtctgcag aacctgcgca ttcgccaggt aatactgctg aaaacgttgc agatttccgt
    26101 actcctcatc gagattgaca ccggaaatcg actgctgctg attggaaagc tgcgtcacca
    26161 cattaccttg cgtggcgcta ctggttttca acgtcgcggt tttattaccg atatcactca
    26221 ctaacgaggc ataagcgtcg ttaaaggatt tcgcaccgcc caccgtttta ctgttgcttt
    26281 gcagatccag cagggcctga ccgttgcggt tatcgctatc acccgcatct tcttcgctcg
    26341 ccatcgctat tttcgcttcg tcggtgatta atacatccat gttgacgata gcgtcactta
    26401 ctggtttcag cgtgaagctg tcgttaacag caggcgttcc tgtaaacgtc aactccagac
    26461 catcaaatgc cactttaccg ttggcatccg gcgtcaccgt aaaagtggta ttactggcaa
    26521 ggcgggtgac ctgccactga ttattatcga acgagatttt gtaatcagtc gccagtaccg
    26581 cggaggcatc agttaccgtg gcaccgatcg caacgtcacc tgtgtttttc gtgttttgca
    26641 gaaccgcggg cttaccgata gcaaagaaat cttcaccagc atcgccgttg gcatcaaatc
    26701 cggctttgtg ttggctgttg aaagcctcgg caaatgccag cgccagttgt ccaagcgtat
    26761 tacgcgtctg gtccagatcc tgagaacgga atgtcagaat gccgcccagc gacccggtat
    26821 tcagtaattt ctccgggatc tcaatattgc ctgccgtccc atcaacgtaa gcgacagtcg
    26881 tacgagaagg gtcggcgctg gaaggaactg ccgccagttg ccgcgccgta cttccctgaa
    26941 ccagtgagta accattggcc atcgtgatgt tataagtacc gccatcctga acgctgactt
    27001 ctacgccaac aatctggttt aattcgctca ccagttgatc gcgttgatcc agcagattgt
    27061 taggtgacgc ccctgccccc acgcctgtca ggcgcgagat ttgatcgttc aggctggcaa
    27121 tttgtttagc gtaattgttg atctgatcaa cgctggcacc tatcgcgata ttgacctgtt
    27181 tgtcctggtc gcgcagatat tgatcggtgg ttttaaactg attcaccaac ccttctgatt
    27241 tcccaatcag cgcctggcgc gctgccgggt cttccgcgtt actcaccagc gtttgcaggc
    27301 tggtgaagaa atcctgcatc tgtgttgcca gcgaagaggt actggtggag agcatattgt
    27361 cgattttcga catctgctca tagcgggcag tcaggccgct actttgcgtc tgcgccgcac
    27421 gtaactggtt ggtaataaaa gcatcatact cacgctgcac accagaaacg tagacaccat
    27481 tgccaaccca gccgccagcg cccaacgtgc tattggcctg cgccataata gtggtttggc
    27541 gggtatatcc ggcaacgtta tagctggaga tattattact tgccgtattt aacgccgcct
    27601 gggccgcgtt cagtccgctc atggcgttat tgatcaagct ggacatggag gttccttata
    27661 agccttcaat tacagagtat tatcggcagc gacccgccgg acttgagtta ttcagaacag
    27721 attatcaatg ttcatgctgt aggttttgct caccttgtcg cttatcgatt tcatctgctg
    27781 gatcatgttg gtgagtttac gggcatagtg aggatcggta gcataccccg cgtcctgtag
    27841 ggcctgcgcc ccctgctccg cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg
    27901 tgttaacagc ccaacgtaat ccgacaatgc ttccagatac gagctgtaga cccgaaactt
    27961 cgcttttact ttcttcgctt cgccgttttc atattcagtc gtggtgattt cagtaactgg
    28021 ccctttccag ttgccagagg ctttgacacc aaacaggtta tagctcggct cgccgttttc
    28081 gcggcggatt tgccgttgcc cccaaccaga ttccagtgcc gcctgagcga ggatcaaatg
    28141 atgtggcaca ccgctttgct ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa
    28201 tgctttactg tcacccggca gcgaatcatc gtagttacgt ggcacggcct tttgcaccag
    28261 ctgcgaaagc gcctgatttt gataacgcac cacagtttcg agcgggaatt tcatcggtgc
    28321 tgctggcgtg gactcctctg gcaatggttg ttctggcgtc atctgtttaa ccatcatctc
    28381 tgcaagcccc agacctttgc ccgccgtcat ctgttgggca atctgctggt catacatact
    28441 ggtatacagg cgagtgtgct cgctgctgaa caggccatct tttggtaaag cgtcgcgcat
    28501 gcttttcaac atcatctgca cgaacatccc ttccacctga cgggccaccg gacggatatt
    28561 tgccgccgga tcttcgcccg ctttcgcctt tagttcgttg agtgattgcg catcccaggc
    28621 cgcacttgcc agtagtttgc tgtcgctgat catcagatga tttccagttt tgcccgcaga
    28681 catcccgcac tttgcattga ttgcagtatg gacatcagat ccatcggcgt agcgcccagc
    28741 gcattgagcg cgcgcaccac gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa
    28801 ccgccgctct ggcgtaaatc gatctgcgtt tgtggagtaa ccacagtctg tccaccacca
    28861 aacggtgtat ctggctggct gacattggcc tgacgattga ctgttactga gagatttccc
    28921 tgcgctaccg cgcagctgtc gagggtcact tcgcgattca tcaccaccga accggtgcgc
    28981 gagttaatca ctactttagc gtcctgcggg gtgacattaa cctgcatatt ctggatatct
    29041 gcaaggaaac ggacctggga actgttgcca cttggtacgc gcacctgaat agtccgcgca
    29101 tctaacgcgg tggcgctgcc atatccacgc acgcggttga tggtgtcagc gatttgctgc
    29161 gccatgctga aatcttcgtc gttaagttgc aaattaaggg tattcccgac gccaaactgg
    29221 ctgggcaatt cacgttcaat aaccgcacca ttggtgatcc gtccaccgtt cagttggttc
    29281 acctgaacac tgctaccgcc agcggaggct cctgcgccgc caaccagaat attgccctgc
    29341 gccagcgcat acacctgact gtcaacgccc ttaagcggcg tcatcaacaa cgtacctcca
    29401 cgcaagcttt tggcatttcc catggaagaa accaccacat cgatggtttg cccctgacgt
    29461 ccaaacggag gaagtgacgc tgtcaccatt accgcagcga cgttttttag ctgcatattg
    29521 gtgcccgtcg gaacggtaat tcccagctgt gagagcatgt tattaagcgt ttgtgtggta
    29581 aacggcgtct gggttgtctg gtcaccggtg ccatccagcc ccaccaccag accatagcca
    29641 atcagtgagt tttgccttac cccctgaaca ctggtgagat cgcgaatacg ctcagcctga
    29701 gccgccgtcg taaccagtag aagaattaat gcagagagaa atttaatcac cacagcctca
    29761 cttacattgg cgacaggtta aggaagaaac gctgcaacca gcccatattt tgcgcttcgt
    29821 taatgtagcc attgcctacg tattcaatgc gcgcatccgc cacctgagta gacggtacgg
    29881 tattgctgcc gctgatagtg cgtggattaa ccacgccaga gaagcgaata aattcggtac
    29941 cctgattaat ggcaatctgt ttttcaccca ccacatgcag gttgccgttg accagtacct
    30001 ggtcaaccgt caccgtcaac gtgccgctaa aggtattgct ggcattggcc ccgccctttc
    30061 cgttgaacgt gttaccaccg gaggcttcga catcggcacg agcgttacca aacagcccct
    30121 gcaaatagcg cggcacagta tcaaagccaa aattagtttt accgtcacgg ctggcattcg
    30181 cagaggagct tttgctggcg ctgacgttct cctgcaacac gatggtcagc gtatcgccaa
    30241 tattgcgtgg tcgacgatct tcaaacagcg gttgatagcc atagttaatc ggctgagcag
    30301 actggaaaat agaaccgttg gcgacgggcg tcggcccggg aaccggttgt gcactggtcg
    30361 ccccctgcac cagcggcgtg gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca
    30421 agctggaaat ggcataagta tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc
    30481 agtaaaccgg tgaacctgcc accggttaag ccttagagtt gcgtcagttt ttgcagcatc
    30541 tgatcggtgg tggacaccgc tttactgttg atttcgtaag cgcgttgcac ctgaatcata
    30601 ttgaccagtt cttccgccac gttgacgtta gacgtttcaa catacccttg atacagcagt
    30661 cccgcgccgt tcaggcccgg cgtgctttcg ttcggtgcac cagaggattg cgtttcggtg
    30721 tagaggtttt cgccaatgct ctccagcccg gtgtcattca taaaggtggt gagattgagc
    30781 tgcccaacct gaaccggagc tgcctggcct tgttgggtta cgctgaccac gccatcacga
    30841 ccgatggtga tacttaacgc attcgccgga atggtgatcg ctggctgcac ctgaaaacca
    30901 ccagccgtca ccagctgccc gttctgatcc acctggaaag agccgtcacg ggtataggct
    30961 gatgaaccat ctggcaacat cacctggaaa aagccctgcc ctttaatcgc gacatctttg
    31021 ctgttgttgg tctgcgacag gtttccctgg ctgtgtaagc gttcagtggc gaccgggcgt
    31081 acccccgtgc cgatttgtaa tccggagggt aaggtggttt gttcggaaga ctgtgccccc
    31141 ggctggcgaa tggtttgata aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg
    31201 ttagtactga cgtttgccag gttgttggca atgacgtcca tattggtttg ctgggcgtca
    31261 aggcccgttt tggcgatcca taatgaactg atcataaaat gtcctgtagt ggattagtga
    31321 caagcagagc agaagcacgc gacagtgtga atttcccctc accctaaccc tctccccaaa
    31381 ggggcgaggg aatagtccgc gcgcttttat tgcaatgtca tcaatcgttc aattaactca
    31441 tcgacagcag ttggttggca cggcctgcgt tatcatcgac gctgctgatc accttcatct
    31501 gcatttcaaa acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt
    31561 tactgccttc cagaaccccc gacatcacac gcaaggttgg atctgcctgc agtatcggcc
    31621 cacgcgtggc ctggctttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgttgcactt
    31681 cgctgcccgt ggctttcacc agtttcagac gccccactgg cgcaaccgta tttgccggat
    31741 cgcccggatt gagcgccgag attgtgccat cggcagcaat agtgatttcc gccccttccg
    31801 gcacagcaat tggcccagcc tcgcctatca ccggatgccc ctgaattgtc agttgcccgg
    31861 tgggatcaac ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggactgca
    31921 cggccagcca gccatcctgc tgcaatgcaa cgtccagcgg gcgcgaggtg taatccatct
    31981 tgccgggcgt catgtctgca cccggtgttg acgccgttac cagcgtgcgc gtgggcagtg
    32041 aaagcccttc caccggcact gcacgcaagg cgtttaactg cgcgcgaaaa ccgggcgtag
    32101 aggcattggc cagattactg gcggttaccg cctgttgatt cagcgtctgg ctggctgctc
    32161 ccatcgcggt ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta
    32221 accagcgtgt tgaggatctg gtcctgggtt ttgatggtct gggcgttaga ctgatagtta
    32281 cgctgggcaa cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccaac
    32341 gcaccgttgg tcagggtgcc aaagttgccc gtcccggctg tccccaacag cgccacgcca
    32401 gaagattgcg tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca
    32461 aagttcgcca gtacaatctg ccccagcagt tgtgtttgtt cgttggaata gttgccgaca
    32521 accgtaccgt catcattgat ttgataactc accagatcgc ccggtttgta gccattctgg
    32581 gtggttgcca caatattgtt agcgccggta ttttgctgca tggagttgag gaagctcagg
    32641 ctaaacgtgg cgggatctgc gccgttaatt gcgccagtgg taatattctc tgttggattt
    32701 gaggtcagaa cgccattggc attaaacacc agcttcattg caggctcggc tgtacctgtt
    32761 ggatcactgc tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaagtag
    32821 acgctcatgt catgagcatt accctgactg tcgaaaacag tcaccgaacc ttttttgtta
    32881 tagctatccg cattgctggc gctgaatggc gtaacagtag gaagcggatc actggaattc
    32941 aggttgatct gcatcgatgc cgtggtggta gttttcgctg ccatcagggt attcgggatc
    33001 gaaatattgg tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa
    33061 cccgtcagct gtaaaccttg catattcacc aggttacggt tttcatccag cttaaattgt
    33121 ccgttacggc tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accgttctgg
    33181 ctgatagcaa cgtccagacc tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga
    33241 gtgataccgg caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa
    33301 gaggccgtgc ctgatttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca
    33361 tcgaggttgg tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac
    33421 tcctgaaatg tgaaggctta aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa
    33481 gtgccgagat ccagcgtatt accgctgttg ccgcggatca caccctgcac cagagcaaac
    33541 tgcagcggct gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa
    33601 gaaccgttcg gcgcagtgct gccgtcagtc aacgtaccgt cccaggtgaa actgtgaact
    33661 ccggcggtca gttcaccaat atcaatggtg cgcacaaccg caccattttt atcggtgatg
    33721 gtggccgtaa ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc
    33781 acagctcctt cttcactgcc ggttcccgca agaacagtgg tgccggggat catcacgccg
    33841 tgaccgatca ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat
    33901 ccgagtgtgg tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac
    33961 gtcagctcgt tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccagc
    34021 aaagtcagaa aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg
    34081 gtggtactga cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc
    34141 tcctttattg accgagcgta agggttttca gcatcatgct tttcaccgtg ttgagcactt
    34201 caacgttggc ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat
    34261 caacgttcgg catttttacg tagccctttg catctgccag cggattaccc ggttcataaa
    34321 ccagtttgtc cggggcctga ctttctataa catcggcaac ctttacgccg cctgtcgcag
    34381 cacctggtgc agcgttaacc tggaatacca cctgttttgc ccgatatggc tgtccatcag
    34441 gaccggtcac gctatcagca tttgccagat tactggccgc cacgttcagg cgctgggact
    34501 gggcagttaa cgccgacccg gcgatatcaa aaatattcag cagtgccatc cgttaatttc
    34561 cgctctgtaa aacgttcatc atgcctttga tttgcccgct caacgcgcta aggctcatct
    34621 ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg atccatatcg acggtattac
    34681 cgtcaagcga aggctggtcc ggaatacggt attgcagttc tgcggtagga ggcgtcagcg
    34741 cctgcgccgg gatgtgttgc gttgaggtca tcgtcagtgc aaccacactg gttgcatccc
    34801 gtccacgttg catgactttt ttaagttcac tggcaaaatc gatatcgcgc gcctgataac
    34861 caggggtatc ggcattggcg atgtttgctg ccagcacttc ctgacgctgg gcgcgcagat
    34921 tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc
    34981 aggtatcaaa attctgccat cagcttaaat gcctttacgc gcgcgttatc ggcggaataa
    35041 acgcaaaatg ggtcgctatt tatgccgttg atggtcattg cggacaggta caattcacgt
    35101 tgtagaaatg gctgggggcg aaaatgctga caataaaacg tagcgtggcg atcatcgcga
    35161 tactgttcag tccgttaagt acggcgagca atctcacatc gcaattgcac aactttttta
    35221 gcgcccaact cgcgggggta agtgatgagg ttcgtgtttc tattcgtaca gcgcccaatc
    35281 tactaccgcc atgcgagcag ccattgcttt cgatgagcaa taattcccgc ctgtggggca
    35341 atgtgaatgt gttggcacgc tgcggtaacg acaaacgata tttacaggtt aatgtgcagg
    35401 ccacaggaaa ttatgtggtt gccgcgatgc ccattgtgcg gggaggaaag ctggaagctg
    35461 gcaatgtcaa actaaaacgc ggacggctgg ataccctgcc accgcgtacg gtgctggata
    35521 tcaatcaact tgttgatgcc attagcctgc gcgatctatc acccgatcaa cctatccagt
    35581 taacccagtt tcgccaggca tggcgggtaa aagcgggaca acgcgtcaat gtgatcgcca
    35641 gcggtgatgg gtttagcgcc aacgcagaag gtcaggcgct gaacaatgca gccgtcgcac
    35701 agaatgcgcg ggtgcgcatg gtatcgggac aggtagtcag cggcgttgtt gatgcagatg
    35761 ggaatattct tataaacctg taagctgtta aagattaccc gtcccttgcc gataaataag
    35821 caacacatga taaaagcgcc ctcaatgagg aataaaccat gagtattgat cgcacttcgc
    35881 ctctgaagcc tgtaagcacc gttcaaccgc gcgaaaccac tgacgcgccg gtaacgaaca
    35941 gccgggcggc aaaaacaacc gcctccacca gcaccagtgt gacgttaagc gacgcgcaag
    36001 caaaactgat gcaacccggc agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac
    36061 tggcgattcg taacggtgaa ctaaaaatgg acaccggcaa aattgccgat gcgctgatca
    36121 acgaagcgca gcaagacttg cagagtaact gatcgtatga cacgtcttgc agagatcctc
    36181 gaccagatgt ccgctgtgct taacgatctc aaaacggtaa tggatcaaga gcagcaacat
    36241 ctctctatgg ggcagatcaa cggcagccag ttgcaatgga ttacagaaca aaaaagctca
    36301 ctgctggcga cgctggatta cctcgaacag ttacgcaaga aagaacccaa tacagcaaat
    36361 agcgttgata ttagtcaacg ctggcaggaa attactgtga aaacgcagca actacgccaa
    36421 atgaatcaac ataacggctg gttactggaa ggacagattg agcgcaatca acaggcgctg
    36481 gaaatgttga aaccgcatca ggaaccgacg ctatatgggg cgaacggtca gacctcaaca
    36541 acccatcgcg gcggtaaaaa gatttcgatc tgaatattat ctccggcctg cactgcaggc
    36601 cggaatgcat tgttacaccg tccggcgggc aaattcttta actttgaagc ccagtaccgc
    36661 cagtgcagcg aagtacgcgg caatccccgc cagcacgacc gccattaaac gcagtaagcg
    36721 ccagggcatg gtacccaatg accactccgg catgatatgt aacataccta aaagcacgcc
    36781 agacatcacc agtaccgcca ccaccagacg caacagaaac gccatccagc cgggttgcgg
    36841 agtaaagatt ttctgcttac gcaactgcca gtaaagcagc gaagcattca gacacgccgc
    36901 cagaccaata gaaagtgaca gcccggcatg tttcaacgga ccaataaacg ccaggttcat
    36961 caattgcgtt aaaatcagcg taacgatggc aattttcact ggcgttttaa tgtcctggcg
    37021 ggaataaaag ccaggagcca acactttcac tacaatcagg ccaatcaaac ccaccgagta
    37081 ggcaattaac gcccgctggg tcatcagcgc atcaaacgcg gtaaatttac cgtactggaa
    37141 cagcgaaacg gttaacggac cagaaagaat gcccaacgca accgcactcg gcagcgccaa
    37201 caggaaacaa agacgcaacc cccagtccat caaacggttg tattcatcat gattgccact
    37261 ggcaaaactt ttcgacagcg acggcagcaa aatggtgcca agcgccacgc ccagcacacc
    37321 ggacggaaac tccattaagc ggtcggcgta atacatccaa gacaccgaac cggaagcaag
    37381 aaacgaggca aaaatggtgt tgatgattaa ggagatctgg ctcacagaga cgccaaggat
    37441 cgccggtccc atctgtttca ccacgcgcat tgttccggca tcgtggaagt taatgcgcgg
    37501 caggaccagc atgccgatct tcttcaggtg cggtagctga tacaccagct gcaggatgcc
    37561 gccgaccgta acagcccacg ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa
    37621 cagcgcgaaa ccaatcatgc tgatgttaag cagtgttgga gcaaacgccg gaatcgagaa
    37681 gcggttccac gtattcagaa tcgctcccac cagcgacgcc agggagatca gaaagatata
    37741 gggaaaggta atctttagta gctggctggt cagggcaaat ttgtcagctg tgtcagcgaa
    37801 gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc atgccagcga ccgtcacaac
    37861 cgccagcgcg agtgtcagca ggccagaaac ataagagaca aagacccgcg tggcgtcttc
    37921 accctgctta cttttatatt ccgccagaat cggtacaaat gcctgggaaa atgccccttc
    37981 ggcaaagata cggcgtaaca agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc
    38041 catccctgcg ccaaagattc tggcgacaat tgcgtctcgt gcgaagccaa gcacacgcga
    38101 aaacatggtc atcgagctga cggcggccag cgattttaat aaattcatcg gtgttctaat
    38161 ccagacccac gggcaaaacg cctgcaaggc aggcgctgaa agaggcgaat agtctacctg
    38221 gattatggtg aattgctacc gccagatgtt acagggttta ttcactcatc gcatcgcgcc
    38281 agatcttgtc aacgatacgt tgcgccagca cggcctgttc gccggcggtt tgcggaactg
    38341 tctgattttg cacacattca atgaagtgcc gcgcacagcc gacaaatcca cgttgttcaa
    38401 gagtgctctg ccaaccagga atcggtttat gcactacgcc ctgcccgcgc tcctcacgcc
    38461 attcgcgcat atccgtaatg tcgatgagcg caccgtcagt cacggcctgc acggtttcac
    38521 gctgacttcc ggcacggcga tgcatacagg tggtgatctg caaaggaccg gccgaaaaat
    38581 ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag tagcgtaccg ccatccagag
    38641 aggctttgcc gcccgacaac cacagcacgg tatccaccac atgcagataa tcatccagca
    38701 acgtgaaata aagatcgtgt ggcccgacac tattgctacg atgtttatcc attcttagcg
    38761 aagctgcggt agcgagttgc gtttttaact caccgtagag tggtgcgaaa cgacggttaa
    38821 aaccgaccat caacgtcagt tttttacgcg ccgccagttc caccagccgt tcagcatcgc
    38881 gcagattttc tgccagcggt ttatcgacac agacatgtac acccgcattg agtaacgtac
    38941 tgaccacgtc aaagtggctg gcggtgctgg aatgcacaaa aaccgcatcg caactggcgg
    39001 caaggctgga taacgaatcg gcataaggaa tgcgccagct ttcacaaatt ggcagggctt
    39061 tcgcgcgcgt aggcgaccag gctccttgta acgtccagtc agacgctgcc gccagcaccg
    39121 gtaaccacgc tttttgcgca atgccaccta atcccactac gccgatacgt aattttttca
    39181 ctttaatctc ccagatgggc cagcaacgaa tcaagacgct gtttcagttc tgccacttcg
    39241 atttccaggg cttcgacgcg ggcctgtaaa tcaccatcaa ccgcgttcga catatccgtc
    39301 accgccggct gatcttcaac ctcaccactg aaaagatgca tgtagcggct ttcgcgttta
    39361 cccggttcgc gggccagacg caccacaaaa ggaccatctt cgcgatttgc cagttgttcc
    39421 agcgtcgact ccacttccgc catatcgctg aattcataca ttcgcgcggc gcggctgcgc
    39481 agttcacctg gcgtctgggc accacgcaat aacaacgtgg tgattaacgc cacttccgct
    39541 gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac gttgctcata tttggtgacc
    39601 cgattaccaa aaccgctcac tgtgcgtaga taatgacgtt tgaccagatt atccagctgt
    39661 tcctgcactt cggattcact cagattcatg accggttcac ggttcgtttt ctgattacag
    39721 gccgtgacta caccattgac tgagagcggg tattgctccg gcgtggtcac ctgcttttcc
    39781 agcaagcaac caatcacgcg agcttcaagg gcagtaagtt gatatttcat gagattctcc
    39841 ttagcggccg ggcgtccagt ctggggtagt taatgccgtc agtacgtgat cgcgccattg
    39901 tccatcaatc aacagatagt ctttcgcata gccttctttt tcaaaaccca gtcgcgccag
    39961 taaatcaccg ctgcgtttat tgtgcggcat ataattagcc ataatgcgat gaatatgttg
    40021 ggtgcgctgc atataacgaa tggctgcggt cagggcttca aacatgagtc ctttgccctg
    40081 ccatttttgc ccaatcgaat aaccgagata gcaggcatga aaagagccac gaacaacatt
    40141 ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg tcgaataagc caaagtagaa
    40201 agctgaacct tgtttatgaa attcgttaat catccccagc ctggcctgcc agcctgatgg
    40261 ataacagtgg ctttcgtcgc gcactggctc ccagggcttg aggaaatggc gattctctgc
    40321 gtaataatcc gcaagacgcc aggcatcacg atcatgcacc agacgcacga ccagtcggtc
    40381 tgtggttaag cgcacttttg gcacgttact gcgatagcca aacattctat acctactcct
    40441 tcccgtaacg tctttaatac gctgtaagta ttactatacc tgcgcaacca gaccctgtga
    40501 aaagtccaaa atgccatttt ctgcattatt cacatatttg ataaaaactc tctatcaaag
    40561 cgacgttttg ataaaaaaat attgtccccg aagtgatggg aaaagtctta gcggcaatgc
    40621 agaatagaaa gtgctcatct tttatttccc gggaggggaa atgtcccgcg tatcgcaggc
    40681 gaggaacctg ggtaaatatt tcctgctcat cgataatatg ctggtcgtgc tggggttctt
    40741 tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa atgggctggg ccgccgtcat
    40801 ggtcggtatt gctctcggtc tacgccagtt tattcagcaa ggtctgggta ttttcggcgg
    40861 cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt accggtatgc tgatgcgcgc
    40921 cgccggattc gccacaatgg gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct
    40981 gctctcggga ctcggcggca cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact
    41041 aatccgtcca caacaacgtg gtcgtttttt ctcgctgttg atgatgcagg acagtgccgg
    41101 tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa tacgactttc gcctggtctg
    41161 cgccacaggg gccgttttgt ttgtgctgtg tgcggcgttc aatgcgtggt tgttaccggc
    41221 atggaaactc tccaccgtac gcacgcccgt tcgcgaaggc atgacccgcg tgatgcgtga
    41281 caagcgtttt gtcacctatg tcctgacgct ggcgggttac tacatgctgg ctgtacaagt
    41341 gatgctgatg ctgccaatta tggtcaacga cgtggctggc gcaccctctg ccgttaaatg
    41401 gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc taccctatcg cccgctggag
    41461 tgaaaagcat tttcgtctgg aacaccggtt gatggctggg ctgttgataa tgtcattaag
    41521 catgatgccg gtgggcatgg tcagcggcct gcaacaactt ttcaccctga tttgtctgtt
    41581 ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc ttaagtgctt cgctggcgga
    41641 cgcaagagct cgcggcagct atatggggtt tagccgtctg ggtctggcga ttggcggcgc
    41701 tattggttat atcggtggtg gctggctgtt tgacctgggc aaatcggcgc accagccaga
    41761 gcttccgtgg atgatgctgg gcattattgg catcttcact ttccttgcgc tgggttggca
    41821 gtttagccag aaacgcgccg cgcgtcgttt gcttgaacgc gacgcctgat ttgatactcc
    41881 cccctgactc ctccatactg aaagtgaact gacagccaaa tggaggagtc acgtgaagct
    41941 atacatttac gatcactgcc cttactgcct caaagcccgc atgattttcg gcctgaaaaa
    42001 tatccccgtc gaattacatg ttctgctcaa cgacgacgca gaaacaccca cccggatggt
    42061 cggtcaaaaa caggttccca ttctgcaaaa agatgacagc cgctatatgc cagaaagcat
    42121 ggacatcgtt cactatgtcg ataaactcga cggcaaaccg ttactgaccg gcaaacgttc
    42181 ccctgccatt gaagagtggc tgcgcaaggt caatggctac gcaaacaaac tgctgttgcc
    42241 gcgttttgcc aaatcggcat ttgatgagtt ttctactccc gccgcgcgca aatatttcgt
    42301 cgacaagaaa gaggccagcg cgggtaattt agccgacctg ctggcccact ctgacggtct
    42361 gattaagaat atcagcgatg atttgcgtgc gctggacaaa ctgatcgtca aaccgaacgc
    42421 cgtgaatggc gaactttcgg aagatgatat tcagctattt ccgctactgc gtaatctgac
    42481 gctggtagcc ggaatcaact ggccaagccg cgttgctgat taccgcgata atatggcgaa
    42541 acagacacaa atcaatttgt tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc
    42601 ctgtattcgc gggttgccgc cgttttcagt tttttctgct ggcgagttta tcgctggtga
    42661 cacatgctgc gttgtgtcag gatattgacg cagatttcat gtcgaggact accatgaaca
    42721 agtttttatt tgccgctgca ttgatcgtca gtggcctgct cgttggctgt aatcaactca
    42781 cccaatacac catcaccgaa caagaaatta accagtcgct tgcgaaacat aataatttct
    42841 caaaagatat cggtttaccc ggcgtggctg acgcccatat tgttctgaca aacttgacca
    42901 gccaaattgg tcgcgaagag ccgaataagg ttaccctaac cggagacgcc aatctggaca
    42961 tgaactccct gttcggtagt cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg
    43021 tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg
    43081 tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct tccctatttg aaccaggcat
    43141 tacgcaatta ctttaaccag caacctgctt acgtcctgcg cgaagatggc agtcagggcg
    43201 aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc
    43261 cgtttacgga ttaatgacga gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac
    43321 gtttccgctt atcctttgtg tccggcaaaa acatcccttc agccggagca tagagattaa
    43381 tgactgcacc atcccaggta ttaaagatcc gccgcccaga cgactggcac cttcacctcc
    43441 gcgatggcga catgttaaaa actgtcgtgc cgtataccag cgaaatttat ggacgggcta
    43501 ttgtaatgcc caatctggct ccgcccgtga ccactgttga ggctgccgtg gcgtatcgcc
    43561 agcgtattct tgatgccgta cctgccgggc acaatttcac cccattgatg acctgttatt
    43621 taacagattc gctggatcct aatgagctgg agcgcggatt taacgaaggc gtgttcaccg
    43681 ctgcaaaact ttatccggca aacgcaacca ctaactccag ccacggcgtg acgtcaattg
    43741 acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg
    43801 gtgaagtgac acatgcagat atcgacattt ttgatcgtga agcgcgcttt atagaaagcg
    43861 tgatggaacc tctgcgccag cgcctgactg cgctgaaagt cgtttttgag cacatcacca
    43921 ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg gctggctgcc accatcactc
    43981 cgcagcatct gatgtttaac cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt
    44041 attgtttacc catcctcaaa cgcaatattc accaacaggc attgcgtgaa ctggtcgcca
    44101 gcggttttaa tcgagtattc ctcggtacgg attctgcgcc acatgcacgt catcgcaaag
    44161 agagcagttg cggctgcgcg ggctgcttca acgccccaac cgcgctgggc agttacgcta
    44221 ccgtctttga agagatgaat gctctgcagc actttgaagc attctgttct gtaaacggcc
    44281 cgcagttcta tggcttgccg gtcaacgaca cattcatcga actggtacgt gaagagcaac
    44341 aggttgctga aagcatcgca ctgactgatg acaccctggt gccattcctc gccggggaaa
    44401 cggtacactg gtccgttaaa caataaaaat cgtagcttcc tgttgtcatt aggttatttt
    44461 acctgtataa ataaccagta tattcaacag ggggctatta tgcgaattga agtcaccata
    44521 gcgaaaactt ctccattgcc agctggggct attgacgccc tggctggcga actttcccgc
    44581 cgtattcagt atgcgtttcc tgataatgaa ggccacgtat cggtacgtta tgccgcagcg
    44641 aataatttat cggttattgg cgcaacaaaa gaagataaac agcgcattag cgaaattctc
    44701 caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg aataatatgc agtgattttt
    44761 gtttttgccg ggtcgccccg gcttttttat tttacttaca aattttgagt acaaaatttc
    44821 tacacttcct caaaataatt gcacaatatg ctaaaaaaat atgaatgaca tgctgtttta
    44881 ttgttcatat aaatcttaaa agcagaaatt tatctgttcc cccctttaaa aattcggtga
    44941 atacccttac ttattggtat actgaagttg cttcagataa aaacgcattg aacctcgaat
    45001 aacgttgtct agtaacacga attagggggc catgatggaa aaaaataatg aagtcattca
    45061 gactcatccg ctcgtagggt gggacatcag caccgttgat agctatgatg cgctgatgtt
    45121 gcgtttgcac taccagaccc caaataagtc cgagctggaa gggactgaag ttggtcagac
    45181 gctctggtta accactgatg ttgccagaca atttatttcg atattagaag caggaatcgc
    45241 caaaattgaa tccggtgatt tcccggtaaa cgagtatcgg cgtcattaac atgacatgac
    45301 catccaccca aaaggcacct caccggtgcc tttaccattt ctgggttgta tatctgcatt
    45361 cagttaatta ctctgcttaa accatgagag cagagagaaa aaaatgaaat acgatctcat
    45421 cattattggc agcggttccg taggcgctgc cgccgggtat tatgcaaccc gcgccggttt
    45481 aaacgtgcta atgaccgacg cccatatgcc accgcatcaa cacggcagcc accatggcga
    45541 tacgcgatta attcgccatg cttatggtga aggcgaaaag tatgtcccgc tggtcctccg
    45601 cgcgcaaacg ctgtgggatg aactttcccg ccacaacgaa gatgatccca tttttgtacg
    45661 ctctggtgtc attaacctcg gcccggctga ctccgcattt ctcgccaacg tcgcccacag
    45721 cgccgaacag tggcaactca acgttgaaaa gctcgatgcg caagggatta tggcccgctg
    45781 gccagaaata cgcgtcccgg acaactacat cggcttattt gagactgatt ccggtttttt
    45841 gcgcagcgaa ctggcgatta aaacctggat ccaactggcg aaggaagcgg gctgtgcgca
    45901 actgttcaac tgcccggtca ccgtaattcg tcatgacgat gatggcgtaa ctattgaaac
    45961 ggttgacggt gagtatcagg cgaaaaaagc gattgtctgc gcgggaacat gggtaaaaga
    46021 cctgctcccg gagctgcctg tccagcccgt acgtaaagta tttgcctggt atcaggccga
    46081 tggccgctat agcgtgaaga ataaattccc ggcgtttacc ggtgaactgc ccaatggcga
    46141 tcaatattat ggttttccgg cagaaaacga cgcgttgaag attggcaaac ataacggagg
    46201 ccaggttatc cattcagcgg atgaacgtgt tccgtttgcg gaagtggtca gcgatggttc
    46261 ggaagccttc ccgttcttgc gcaatgtatt gccgggtatc ggttgctgcc tgtacggcgc
    46321 tgcctgcacc tatgataatt cgcctgacga agattttatt atcgataccc tacccggcca
    46381 cgataataca ctgctcatta ccggcctgag tgggcacggt tttaaatttg cgtcagtttt
    46441 aggggaaata gctgccgatt ttgcgcaaga caaaaaaagc gattttgatt tgacgccatt
    46501 caggctttcc cgcttccaat aatcatcatt acggcctcca gaatgaggcc gtttttacag
    46561 gagtatttaa tgcgtcggct gttgcactat ctcatcaata atattcgcga gcatctgatg
    46621 ctttatcttt ttctctgggg attgttggca attatggatc tgatctatgt gttttatttt
    46681 tagttttccg cactgacata aactgacaaa tatttaatct ccctttcatt tggcgtgaaa
    46741 aattgaatga gattattcct gcttctacag cactcaccag acatctctga tgctgaaccc
    46801 actaaactta tgaagattct ttcattatta attcaaaatt attgaaataa aaataaatat
    46861 cattcaattt gttatttttg tgttgcaatt cattctccga cacgccattt tctttctctc
    46921 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
    46981 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
    47041 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
    47101 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
    47161 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
    47221 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
    47281 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
    47341 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
    47401 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
    47461 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
    47521 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
    47581 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taacttccgc atccagcatc
    47641 ttggctatag ctggttatat ggcaccttta aagatttcga cggtactttt acctttgacg
    47701 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
    47761 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
    47821 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
    47881 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
    47941 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
    48001 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
    48061 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
    48121 gcaatgatat ttattccgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
    48181 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
    48241 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcac
    48301 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
    48361 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
    48421 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
    48481 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
    48541 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
    48601 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
    48661 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
    48721 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
    48781 actcgccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
    48841 cgcgtacctt catgcgcagt acccagaaag atttcccgtc atcatccaac gcgatattca
    48901 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
    48961 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
    49021 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
    49081 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcagcc aacattttgg
    49141 cttttagcgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
    49201 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
    49261 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagtgcg gcaatttcgc
    49321 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
    49381 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
    49441 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
    49501 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
    49561 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
    49621 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggcctgatg
    49681 gaaaccggca tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
    49741 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
    49801 cattttctga cactggagct gggtgcgcgg cagtttggta tgcaggaacc ggggattggc
    49861 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
    49921 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
    49981 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
    50041 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
    50101 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
    50161 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
    50221 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
    50281 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
    50341 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
    50401 gctctctgga ttgcgccccc tggaagtcga gcgcataatt agtgtgctta tcttttcttc
    50461 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tgaaaatgac
    50521 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
    50581 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
    50641 ggtcactccg ccctgaatat gtggtccggt attgtcttca gcattacatt tttattttcg
    50701 gccatcgcct caccgttttg gggtggactc gccgaccgta aaggccgaaa actcatgcta
    50761 ttacgctctg ccctcggcat gggcatcgtg atggtgttga tgggactggc acaaaatatc
    50821 tggcagtttt tgatcctacg ggcgcttctt gggttacttg gcggatttgt ccccaacgct
    50881 aatgctctta tcgccacaca agtaccgcgt aataaaagcg gctgggcgct gggtacgctc
    50941 tccacaggcg gcgttagtgg tgcgttgctc ggcccaatgg ctggcggcct gctcgccgat
    51001 agctacggct tacgtccggt attctttatt accgccagtg tgctcatact ctgctttttc
    51061 gtcaccctgt tctgcatcag agaaaaattc cagccggtca gcaaaaaaga gatgctgcac
    51121 atgcgggaag tggtgacatc acttaaaaac ccgaaactgg tactcagcct gtttgtcacc
    51181 acgttaatca tccaggtggc gacaggctca attgccccca ttctgacgct gtatgtccgc
    51241 gaactggcgg gtaacgtcag taacgtcgcc tttatcagtg gcatgatcgc ctcggtgcca
    51301 ggcgtggcgg ctctgctaag cgcaccacga ctcggcaaac ttggcgatcg aatcggaccc
    51361 gaaaagatcc tgattacagc gctgatcttt tctgtactgc tgttgatccc aatgtcttac
    51421 gttcagacgc cattgcaact tgggatttta cgttttttgc tcggtgccgc cgatggtgca
    51481 ctactccccg ccgtacagac actgttggtt tacaactcga gcaaccagat cgccgggcgt
    51541 atcttcagct ataaccaatc gtttcgtgat attggcaacg ttaccggacc attgatggga
    51601 gcagcgattt cagcgaacta cggtttcaga gcggtatttc tcgtcaccgc tggcgtagtg
    51661 ttattcaacg cagtctattc atggaacagt ctacgtcgtc gtcgaatacc ccaggtatcg
    51721 aactgatttt tcgcctttca tacttgcaaa agcggagaat cagctatcct tttccctgaa
    51781 acctcatcaa ctcaaaggga gaatcgtgat gaccatgtac gcaacgcttg aagaagccat
    51841 tgacgctgca cgcgaagaat ttcttgcaga caaccccggc atcgacgccg aagatgcgaa
    51901 tgtgcaacag ttcaatgccc aaaaatacgt tttgcaggac ggcgacatca tgtggcaagt
    51961 tgagtttttt gccgacgaag gggaagaagg tgaatgttta cctatgctta gcggtgaagc
    52021 cgcgcaaagt gtttttgatg gcgactatga tgagatagag atacgccagg agtggcagga
    52081 agagaataca ttacatgaat gggacgaggg ggaatttcag cttgagccac cgctggatac
    52141 cgaggaagga cgcgcagcag ctgatgagtg ggatgaacgt taatcactca tacgggccat
    52201 gatgaatgtc gatcggcagc agtaaagtat caaaaaccag agagaatggc agatcaagga
    52261 tcgtgacata acgccaggcg gagtcacgca catcccattg cacaccggga taatattggt
    52321 tgccatgccc ctgccccggt atagtgcgac taataatgct gccacagcca ctcagcaagg
    52381 tcaccatgat gctcaccaca attaaacgca tttgatacct ctttcgtaaa aaaataccgg
    52441 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcggcgtg aacgccttat
    52501 ccgacccaca aaatcttgca aaatcaataa attgcaggaa cgatgtaggc ctgataagcg
    52561 tagcgcatca ggcaactacg tttttattgc gaagccgcat ccggtttacg caatgccagt
    52621 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc cggggaattc
    52681 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac caggcgacga
    52741 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
    52801 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
    52861 aatgacgggt taaacactgc gtgcataaag ccatcatcaa gggagcgttg acgattcatc
    52921 tcaaggaacc gatcggtatc aaccagcacc tgcggcggcg aatactcttc cgggatcagg
    52981 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
    53041 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
    53101 aaccacgcca taccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
    53161 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
    53221 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
    53281 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
    53341 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
    53401 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
    53461 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
    53521 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
    53581 gtcaggaata ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
    53641 atcaggttac cgtggcacca gcggcggtca cgttttagct catcaagcaa gttaggcggc
    53701 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
    53761 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
    53821 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
    53881 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
    53941 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
    54001 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac caggccgcat
    54061 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
    54121 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
    54181 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
    54241 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
    54301 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
    54361 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
    54421 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
    54481 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
    54541 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
    54601 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
    54661 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
    54721 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
    54781 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
    54841 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
    54901 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
    54961 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
    55021 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
    55081 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
    55141 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
    55201 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
    55261 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
    55321 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
    55381 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
    55441 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
    55501 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
    55561 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
    55621 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
    55681 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
    55741 ctccatcgag aagctgctga ccgctaaatg tttcgggtta ttcaacggac gccagatcca
    55801 ttcgccatta ccggcatgga tagacagacc gttagagtcg tgcaactccg gacgatagtt
    55861 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
    55921 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
    55981 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
    56041 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
    56101 aaattcttca cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
    56161 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
    56221 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
    56281 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
    56341 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
    56401 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
    56461 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
    56521 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
    56581 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
    56641 acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt
    56701 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
    56761 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
    56821 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
    56881 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
    56941 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
    57001 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
    57061 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
    57121 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaacca
    57181 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
    57241 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
    57301 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatccccct actgacatta
    57361 ccgcaattta ttatgctgca atatgtcaaa ggaaaagcgg aaagttggcc tgggctgtca
    57421 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
    57481 gtgttagtag tcatgacgac gctgtgcgta tggatattta agcgcatcag aaataattta
    57541 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
    57601 tgcctcggca tcggttatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
    57661 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tgttttattt gccgttcttt
    57721 atcctcggcg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
    57781 cgtggctgta cccttgcagc agcattggcg tttgtcgctt atttactcaa ccagcgctat
    57841 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
    57901 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
    57961 gtgacttatt ttgttaacgc atcgctgttt atctatctgg ttcaccaccc gttaacgctg
    58021 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
    58081 ctgatatttg tagtagggat tgcgataatt ctgtatgaaa ttcatttgcg catcccgtta
    58141 ctgaagtttt tgttctctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
    58201 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
    58261 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
    58321 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
    58381 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
    58441 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
    58501 ttttaccatc gatgctaaag gttttagcat gcaggctggc tccggaatta ccggttatgc
    58561 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
    58621 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
    58681 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
    58741 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
    58801 tgggtgagcc catgatatcg aacaggcgct gcggtaacag tgaatgccgt tttgccttgc
    58861 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
    58921 ttccatcaac cagatgattt ataaacggac tggattccat tttgcgtaag taacgatgcg
    58981 tcatagcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
    59041 ggacatccag cacttgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
    59101 catcggcaac gtcctctacc acgggcccta ttgccataac atctaaatcc gaaaaaagtg
    59161 gctcttcccc tgccccaaaa taggcatcac caatatttcg cccccctacc agcgtcacca
    59221 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
    59281 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
    59341 ggggatggct gtcaagcaag cgtaaaatgt cgtcaagtcc gggcgtattg ttgtcgtcca
    59401 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
    59461 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
    59521 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
    59581 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
    59641 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
    59701 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
    59761 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
    59821 gccactgacg tatagctgtt tgccgccacc agtcgtaggc tattgagata ggcatcctgc
    59881 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgcactacg
    59941 gctttagcgg gaagagcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
    60001 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
    60061 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
    60121 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
    60181 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
    60241 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
    60301 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
    60361 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
    60421 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
    60481 atggatcacc cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaagcaaact
    60541 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
    60601 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
    60661 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
    60721 ccattgttgt gataaatgaa gtgactgtcc atcagaaaca gtaacaacta ttttcacccg
    60781 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
    60841 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
    60901 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
    60961 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
    61021 cgcattcata ttcttctccc gaaaaaaaac agggcttgcg ccctgtttct gtaatacaaa
    61081 tgatgtatta gtactgatga gcggtcgcgt tgttaccaaa gccaacctga gtcacgttga
    61141 cggaggagtt agatgcagtc tggtcaactg cagcaccgtt gccaccaccg aactgtttaa
    61201 cagtcatttc agaatttttg ccgttccact gatcaagagt ggcgctgtta ccgaagccac
    61261 gttgggtcag atcgattgag ctgtcatctg agccctgacc tacatctgca ccattaccac
    61321 cgccatgctg ggtaatagtc aagtcagagt tacgggcatc agtttgcaga gcaagtgcag
    61381 agttaccgcc accgtactgg taaatgttca gctctgaatt tgggccgcta ttattaccgc
    61441 caccaccgtg gttaccgccg ccgccgtact gaggaacaac acctgccaga gcgctaccgg
    61501 agaatacgat tgctgcaatt gctgctactt ttaaaagttt catgtaaaac ccccatcgga
    61561 ttgatttaaa agtcgaatgg aaattaacgt tgtgtcacgc gaatagccat ttgcgactgt
    61621 ctctgcacta caattgccgt tttttgagta ccatactgtg taatatttgc tttattacca
    61681 gaacctttct ggataatcat cgcagtatta ccataagcac cttgcgaaat actggcatcg
    61741 ttggcactgc ccgcctgatc aatatatgca aggttataat ctcctgtctg gtcaatcttt
    61801 gcccggttgc tactaccttc ttgcgcaaca accgccaaaa gttttgagcc tccctgccgt
    61861 aactgagcac tattattcgt cccagcttga ccaattatgg ctgcctgatt aaatgaagac
    61921 ttactcaatt catttaccgc gaagttatat tctgaattag ctaaatcata acctgctgcg
    61981 gctgcaatcc caggcgcacc cagtattgtt aacatcataa ataacaattt gtttttcatg
    62041 ttgtcaccct ggacctggtc gtacatttaa gaaattaaat catttcaact tggttgttaa
    62101 cgcaacctgt attttgttaa cgctgcgtta cgatggaaag tatgtctgcg gaaatatttt
    62161 taataactca cccgcgcgtt gtatttttct ttttttgttc ataccaaagt attaaaaccc
    62221 acgtaaatac gctgttatct acgcaaaaaa tattttgttt tcttttaaat ctccgttttc
    62281 cgctatcaaa aatcaccaga cagtcatttt tcttgcccgt cgctgattgc tgcgatatgt
    62341 cttgcgcaca agccgtgaca aacctcgtct ttttgcagta aaaaattgtc cacggaggtg
    62401 cggagaa
//
LOCUS       DAZFJF010000013        61526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFJF010000013 DAZFJF010000000
VERSION     DAZFJF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.013"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            21..145
                     /locus_tag="ACHKAU_000963"
     ncRNA           21..145
                     /ncRNA_class="other"
                     /locus_tag="ACHKAU_000963"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(254..1138)
                     /gene="xapR"
                     /locus_tag="ACHKAU_000964"
     CDS             complement(254..1138)
                     /gene="xapR"
                     /locus_tag="ACHKAU_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO5530076.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(1179..1337)
                     /locus_tag="ACHKAU_000965"
     CDS             complement(1179..1337)
                     /locus_tag="ACHKAU_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO5530077.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(1390..2646)
                     /gene="xapB"
                     /locus_tag="ACHKAU_000966"
     CDS             complement(1390..2646)
                     /gene="xapB"
                     /locus_tag="ACHKAU_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO5530078.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(2706..3539)
                     /gene="xapA"
                     /locus_tag="ACHKAU_000967"
     CDS             complement(2706..3539)
                     /gene="xapA"
                     /locus_tag="ACHKAU_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO5530079.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            3788..4552
                     /gene="yfeN"
                     /locus_tag="ACHKAU_000968"
     CDS             3788..4552
                     /gene="yfeN"
                     /locus_tag="ACHKAU_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO5530080.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(4591..5517)
                     /gene="yfeR"
                     /locus_tag="ACHKAU_000969"
     CDS             complement(4591..5517)
                     /gene="yfeR"
                     /locus_tag="ACHKAU_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5530081.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGEPLYQI"
     gene            5607..6605
                     /gene="yfeH"
                     /locus_tag="ACHKAU_000970"
     CDS             5607..6605
                     /gene="yfeH"
                     /locus_tag="ACHKAU_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO5530082.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(6602..6820)
                     /gene="ypeB"
                     /locus_tag="ACHKAU_000971"
     CDS             complement(6602..6820)
                     /gene="ypeB"
                     /locus_tag="ACHKAU_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO5530083.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(6822..8837)
                     /gene="ligA"
                     /locus_tag="ACHKAU_000972"
     CDS             complement(6822..8837)
                     /gene="ligA"
                     /locus_tag="ACHKAU_000972"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO5530084.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(8908..9894)
                     /gene="zipA"
                     /locus_tag="ACHKAU_000973"
     CDS             complement(8908..9894)
                     /gene="zipA"
                     /locus_tag="ACHKAU_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO5530085.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            10124..10885
                     /gene="cysZ"
                     /locus_tag="ACHKAU_000974"
     CDS             10124..10885
                     /gene="cysZ"
                     /locus_tag="ACHKAU_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO5530086.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            11070..12041
                     /gene="cysK"
                     /locus_tag="ACHKAU_000975"
     CDS             11070..12041
                     /gene="cysK"
                     /locus_tag="ACHKAU_000975"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO5530087.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            12425..12682
                     /gene="ptsH"
                     /locus_tag="ACHKAU_000976"
     CDS             12425..12682
                     /gene="ptsH"
                     /locus_tag="ACHKAU_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO5530088.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            12727..14454
                     /gene="ptsI"
                     /locus_tag="ACHKAU_000977"
     CDS             12727..14454
                     /gene="ptsI"
                     /locus_tag="ACHKAU_000977"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO5530089.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            14495..15004
                     /gene="crr"
                     /locus_tag="ACHKAU_000978"
     CDS             14495..15004
                     /gene="crr"
                     /locus_tag="ACHKAU_000978"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO5530090.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(15046..15897)
                     /gene="pdxK"
                     /locus_tag="ACHKAU_000979"
                     /gene_synonym="thiJ"
     CDS             complement(15046..15897)
                     /gene="pdxK"
                     /locus_tag="ACHKAU_000979"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO5530091.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            16002..16370
                     /gene="yfeK"
                     /locus_tag="ACHKAU_000980"
     CDS             16002..16370
                     /gene="yfeK"
                     /locus_tag="ACHKAU_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO5530092.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(16373..17284)
                     /gene="cysM"
                     /locus_tag="ACHKAU_000981"
     CDS             complement(16373..17284)
                     /gene="cysM"
                     /locus_tag="ACHKAU_000981"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO5530093.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(17418..18515)
                     /gene="cysA"
                     /locus_tag="ACHKAU_000982"
     CDS             complement(17418..18515)
                     /gene="cysA"
                     /locus_tag="ACHKAU_000982"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO5530094.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(18505..19380)
                     /gene="cysW"
                     /locus_tag="ACHKAU_000983"
     CDS             complement(18505..19380)
                     /gene="cysW"
                     /locus_tag="ACHKAU_000983"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO5530095.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            complement(19380..20213)
                     /gene="cysT"
                     /locus_tag="ACHKAU_000984"
     CDS             complement(19380..20213)
                     /gene="cysT"
                     /locus_tag="ACHKAU_000984"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO5530096.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(20213..21229)
                     /gene="cysP"
                     /locus_tag="ACHKAU_000985"
     CDS             complement(20213..21229)
                     /gene="cysP"
                     /locus_tag="ACHKAU_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO5530097.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(21400..22191)
                     /gene="ucpA"
                     /locus_tag="ACHKAU_000986"
     CDS             complement(21400..22191)
                     /gene="ucpA"
                     /locus_tag="ACHKAU_000986"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO5530098.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(22320..23177)
                     /gene="murR"
                     /locus_tag="ACHKAU_000987"
     CDS             complement(22320..23177)
                     /gene="murR"
                     /locus_tag="ACHKAU_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO5530099.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            23341..24237
                     /gene="murQ"
                     /locus_tag="ACHKAU_000988"
     CDS             23341..24237
                     /gene="murQ"
                     /locus_tag="ACHKAU_000988"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO5530100.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            24241..25665
                     /gene="murP"
                     /locus_tag="ACHKAU_000989"
     CDS             24241..25665
                     /gene="murP"
                     /locus_tag="ACHKAU_000989"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO5530101.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            25670..26974
                     /gene="pbp4b"
                     /locus_tag="ACHKAU_000990"
     CDS             25670..26974
                     /gene="pbp4b"
                     /locus_tag="ACHKAU_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO5530102.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(27032..27931)
                     /gene="yfeX"
                     /locus_tag="ACHKAU_000991"
     CDS             complement(27032..27931)
                     /gene="yfeX"
                     /locus_tag="ACHKAU_000991"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO5530103.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(28027..28602)
                     /gene="yfeY"
                     /locus_tag="ACHKAU_000992"
     CDS             complement(28027..28602)
                     /gene="yfeY"
                     /locus_tag="ACHKAU_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO5530104.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(28663..29112)
                     /gene="yfeZ"
                     /locus_tag="ACHKAU_000993"
     CDS             complement(28663..29112)
                     /gene="yfeZ"
                     /locus_tag="ACHKAU_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO5530105.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(29099..29524)
                     /gene="ypeA"
                     /locus_tag="ACHKAU_000994"
     CDS             complement(29099..29524)
                     /gene="ypeA"
                     /locus_tag="ACHKAU_000994"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO5530106.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            29738..30607
                     /gene="amiA"
                     /locus_tag="ACHKAU_000995"
     CDS             29738..30607
                     /gene="amiA"
                     /locus_tag="ACHKAU_000995"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO5530107.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            30611..31510
                     /gene="hemF"
                     /locus_tag="ACHKAU_000996"
     CDS             30611..31510
                     /gene="hemF"
                     /locus_tag="ACHKAU_000996"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO5530108.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(31516..32568)
                     /gene="eutR"
                     /locus_tag="ACHKAU_000997"
     CDS             complement(31516..32568)
                     /gene="eutR"
                     /locus_tag="ACHKAU_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO5530109.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(32614..33114)
                     /gene="eutK"
                     /locus_tag="ACHKAU_000998"
     CDS             complement(32614..33114)
                     /gene="eutK"
                     /locus_tag="ACHKAU_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO5530110.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(33127..33786)
                     /gene="eutL"
                     /locus_tag="ACHKAU_000999"
     CDS             complement(33127..33786)
                     /gene="eutL"
                     /locus_tag="ACHKAU_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO5530111.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(33796..34683)
                     /gene="eutC"
                     /locus_tag="ACHKAU_001000"
     CDS             complement(33796..34683)
                     /gene="eutC"
                     /locus_tag="ACHKAU_001000"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO5530112.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(34704..36065)
                     /gene="eutB"
                     /locus_tag="ACHKAU_001001"
     CDS             complement(34704..36065)
                     /gene="eutB"
                     /locus_tag="ACHKAU_001001"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO5530113.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(36077..37480)
                     /gene="eutA"
                     /locus_tag="ACHKAU_001002"
     CDS             complement(36077..37480)
                     /gene="eutA"
                     /locus_tag="ACHKAU_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO5530114.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(37477..38703)
                     /gene="eutH"
                     /locus_tag="ACHKAU_001003"
     CDS             complement(37477..38703)
                     /gene="eutH"
                     /locus_tag="ACHKAU_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO5530115.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(38820..40007)
                     /gene="eutG"
                     /locus_tag="ACHKAU_001004"
     CDS             complement(38820..40007)
                     /gene="eutG"
                     /locus_tag="ACHKAU_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO5530116.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSNAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(39997..40833)
                     /gene="eutJ"
                     /locus_tag="ACHKAU_001005"
     CDS             complement(39997..40833)
                     /gene="eutJ"
                     /locus_tag="ACHKAU_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO5530117.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(40844..42247)
                     /gene="eutE"
                     /locus_tag="ACHKAU_001006"
     CDS             complement(40844..42247)
                     /gene="eutE"
                     /locus_tag="ACHKAU_001006"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO5530118.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(42259..42546)
                     /gene="eutN"
                     /locus_tag="ACHKAU_001007"
     CDS             complement(42259..42546)
                     /gene="eutN"
                     /locus_tag="ACHKAU_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO5530119.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(42653..42946)
                     /gene="eutM"
                     /locus_tag="ACHKAU_001008"
     CDS             complement(42653..42946)
                     /gene="eutM"
                     /locus_tag="ACHKAU_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO5530120.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(42985..44001)
                     /gene="pta"
                     /locus_tag="ACHKAU_001009"
     CDS             complement(42985..44001)
                     /gene="pta"
                     /locus_tag="ACHKAU_001009"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5530121.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(43998..44801)
                     /gene="eutT"
                     /locus_tag="ACHKAU_001010"
     CDS             complement(43998..44801)
                     /gene="eutT"
                     /locus_tag="ACHKAU_001010"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO5530122.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(44798..45499)
                     /gene="eutQ"
                     /locus_tag="ACHKAU_001011"
     CDS             complement(44798..45499)
                     /gene="eutQ"
                     /locus_tag="ACHKAU_001011"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO5530123.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(45474..45953)
                     /gene="eutP"
                     /locus_tag="ACHKAU_001012"
     CDS             complement(45474..45953)
                     /gene="eutP"
                     /locus_tag="ACHKAU_001012"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO5530124.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(45966..46301)
                     /gene="eutS"
                     /locus_tag="ACHKAU_001013"
     CDS             complement(45966..46301)
                     /gene="eutS"
                     /locus_tag="ACHKAU_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO5530125.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(46594..48873)
                     /gene="maeB"
                     /locus_tag="ACHKAU_001014"
     CDS             complement(46594..48873)
                     /gene="maeB"
                     /locus_tag="ACHKAU_001014"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO5530126.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            49162..50112
                     /gene="tal"
                     /locus_tag="ACHKAU_001015"
     CDS             49162..50112
                     /gene="tal"
                     /locus_tag="ACHKAU_001015"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5530127.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            50132..52135
                     /gene="tkt"
                     /locus_tag="ACHKAU_001016"
     CDS             50132..52135
                     /gene="tkt"
                     /locus_tag="ACHKAU_001016"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5530128.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(52231..53274)
                     /gene="ypfG"
                     /locus_tag="ACHKAU_001017"
     CDS             complement(52231..53274)
                     /gene="ypfG"
                     /locus_tag="ACHKAU_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO5530129.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(53400..53975)
                     /gene="nudK"
                     /locus_tag="ACHKAU_001018"
     CDS             complement(53400..53975)
                     /gene="nudK"
                     /locus_tag="ACHKAU_001018"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO5530130.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(54043..56022)
                     /gene="aegA"
                     /locus_tag="ACHKAU_001019"
     CDS             complement(54043..56022)
                     /gene="aegA"
                     /locus_tag="ACHKAU_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO5530131.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            56228..57928
                     /gene="narQ"
                     /locus_tag="ACHKAU_001020"
     CDS             56228..57928
                     /gene="narQ"
                     /locus_tag="ACHKAU_001020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO5530132.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            58092..61205
                     /gene="acrD"
                     /locus_tag="ACHKAU_001021"
     CDS             58092..61205
                     /gene="acrD"
                     /locus_tag="ACHKAU_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO5530133.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(61304..61363)
                     /gene="ypfM"
                     /locus_tag="ACHKAU_001022"
     CDS             complement(61304..61363)
                     /gene="ypfM"
                     /locus_tag="ACHKAU_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO5530134.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        14447 a        16962 c        15676 g        14441 t
ORIGIN      
        1 cgccctaaag gcgctttttt gctatctgcg atacttaaag attcgaacct gcagcaggtt
       61 tgagttgagc gcagcgaaac aacggagccg ctcgcggcga cggcccgaag ggcgagcgaa
      121 gcgagtcatc ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta
      181 tctgcgattt gcgaaattgc ctgatgcgct tcacttagca gacgactatt tccggcaatt
      241 cctgtctcct cacctactgt gtcaatgcag ccaacagctt aaccatcgcg ggcgtcacct
      301 gctgtgtttc ataaacaata tataaatctg cagggatgcg ctgtttgagc ggacggaaaa
      361 tgacacctgg ccagttcatt tgtgcgtagc tgtccgctat caatgtgata ccaataccca
      421 tactgaccat ggcgagtacc gtttgcggtt cattaacttc gcgaataaca accggtgaaa
      481 atcccacctg ctggcaaact cgctgcaaaa aatcccagtc agtgtacacg ggcggcattg
      541 taacaaaata ctcgtcacgt agcgcttcca gcggaacggt ggaaaatgat gagagatgat
      601 gctcttcagg catcgccacc agaaacgccg attcatgcaa ccgtaagctg gtaaaacctg
      661 tcggtggttc tgtcgccatt cgccagatcc cggcatcgag ttcgcgtcgt tccagcaggg
      721 ccatttgcat cgcgggcatc ttttcgcgaa aaagaacttc aacgttagga ttttccctga
      781 ggaatcgccg cataaccggg cgcatccgtc cccacatagc cgttcccact acgccgagtt
      841 caatccgccc tgcttctccc cgacctattt gttcaacccg agccaataca ttattagcat
      901 tcaccagcaa tcgacgcgat tcttccatca agattttgcc cgcgtgtgtc agtacgacgc
      961 tgcgcgaatg gcgaataaaa agctgcgtgc cgagttgatt ttccagctct ttaatatgaa
     1021 tactgagcgg aggctgagac atatttaaac gcgctgctgc gcggccaaaa tgcaactctt
     1081 ccgctacggc aagaaaataa cggagcaact taagatctgt tctgtatacg cgttccataa
     1141 ttaagtgctc catataataa atacatcgta ttaaattatc atggcataaa gtatttcacc
     1201 accagatatc cgacatacat aaacacaatg gataatatac ctggtaatac atttggtaca
     1261 ggaataggca agcgcacgat tgtaaacaaa gcaccaatgc caaaaccaac gccggaagaa
     1321 aataaaatat ctttcactaa ttaaccttta tcataaaagc agctctgaag agcagagctg
     1381 cgaatccttt taatgagcca ccgctcgatg ctttatcttt tcagggtcat gattatattt
     1441 aaacccaaag aaaaatatca ctgcgagaaa aagagcatat cctgcaaaca ccagccagat
     1501 agtttgccag tcttttacgc catccaccga aaagtaatct actgccatgc cactcagaat
     1561 cgagccaacc catgcgccga caccatttac catggtcata aagagcccct gcgcgctggc
     1621 acgaatgctg gaatcaactt cctgttcgac aaataccgaa ccagaaatat tgaagaaatc
     1681 gaatgcacag ccataaacaa tcatcgacag cagcagcaaa ataaatccgg ttgttgacgg
     1741 atcgccatag gcgaagaagc caaagcgcag cgtccaggcc accatactca tcagcatgac
     1801 ggttttaatg ccaaatcgct ttaaaaagaa tgggatagtc agtataaagc ccacttctgc
     1861 catctgtgaa actgacagta aaatggaggg atatttcacc acaaaactgt cagcaaactc
     1921 cgggttacgg gcgaaatcat gtaggaacgg attaccaaaa acgttggtaa tttgcagtac
     1981 cgcacccagc atcatggcaa agaggaaaaa gatggccatg cgtggatttt taaacagcac
     2041 gaaggcatcc agacccagct tgctggcaag cgatgtggtc gcttttttct ccgcaaccgg
     2101 aatcttcggc aaagtcagcg cataagccga cagcagcaat gacgcaccgg acgcgatata
     2161 cagctgcaga ctactcaatt ccagatgcag caggcttact gcccacatcg cgacaatgaa
     2221 ccccaccgta ccaaaaacgc gaatgggcgg gaaagcggtc accgggtcaa gccctgcctg
     2281 ggcaagacag gaataagaga cgctgttcga taacgcaata gtcggcataa acgccatcgc
     2341 attgactaac atcacccaaa acatcatatc cggatcagtt acggatgccg cataaaaaag
     2401 tacgcccgca cacaccaggt gacacagcat gtatgcacgt tctgcgcgca gccatttgtc
     2461 tgcgatgatc cccattatac caggcataat aatcgcggcg atccctttgg aactgtaaac
     2521 catgccaaca ttagcgccgg tgaaatgaag ggtattaatc atgtaagagc cgagggtaac
     2581 cagccagctc ccccagataa aatattgcaa aaaggacatt acctttaagc gcatcgcgat
     2641 gctcataatc gtttccttgt cgcatcattt ggcctcaccc gcgaggccgt ttcttattgt
     2701 ttttctcagg caattttgcg cagaaagcca caaataaggt tgatgaagtt ctgcttagag
     2761 agctccgcag ccgccagcgt ttgcgcatgt gacaatttca catcgcttaa accttccgcc
     2821 atattggtaa tcgcagagac cgcaacgact ttaaggtcgc aatggcgagc tgaaataacc
     2881 tcaggcacca cagacatacc aacaacatcc ccaccaataa tttgcatcat gcgaatttcc
     2941 gccgcagtct cgaaattcgg ccccggatac gagacgaaca cgccctccgt cagagggaaa
     3001 ccctcttctt tcgccacttt ttgtaacagt gcgcggtatt ccgcatcgta ggcatttgcc
     3061 agtgagaaga agcgctctcc aaaacgatca tcgttaagac ccaccatcgg cgtaccgggc
     3121 atggtgttga tatgatcttt caatgcgacc aggctgcctg cccccacttc cggacgcagc
     3181 gagcctgccg cattggtgca gaacagtagc tcgcagccca gcagcttaaa ggtacgaatt
     3241 gcgtcagtca taatggtcat tccacgacct tcgtagaaat gtccgcgacc tttcatacat
     3301 accaccggta cgccctggag atggcccagc actaactccc ccgcatgacc atgtaccgta
     3361 cttaccggga agcccggcag cttttcgtag gaaattgcga cagcgttctc aatctgatcg
     3421 gccagcgcgc cgagcccgga acctaaaata aaggccactc gtggcgtgaa atcaggttta
     3481 taagtcttga taatatctat gcaaaacagc gggttatgag aaaattgaac ctgagacata
     3541 catatccttt ttctgtaggg tggaatctaa cgccttttta tagcgaggtt gtcccggctc
     3601 ttaccaatac acttttctca cgcattcaat agcgattcga tatcgatgga tttatatctt
     3661 ttctctatta atccacggta aaaactgtat tggcgatatt aattcctttg cattaacaat
     3721 atgtcgtttt tagcaaacca ctttattaag caatctacta ataacgatcg gcatagggaa
     3781 ttatgctatg aaaaaacatc ttttaactct gacactttcc tctatattag cgatacctgt
     3841 tgtatctcat gcagaattta aaggcggttt tgcagatatc ggcgtgcatt atctggactg
     3901 gaccagccgg actaccgaaa aatcgtcgac caaatcacac aaagatgatt ttggttatct
     3961 tgaatttgaa ggtggcgcta actttagttg gggagaaatg tacgggttct tcgactggga
     4021 aaacttctat aacggtcgcc ataataaacc aggtagcgag cagcgttata cctttaaaaa
     4081 taccaaccgt atttacctgg gtgacaccgg atttaatctc tacttgcatg cgtatggcac
     4141 ctacggttct gcgaatcgcg tgaatttcca cgacgatatg ttcctgtacg gtatcggcta
     4201 caatttcacc ggcagcggtt ggtggttcaa accgttcttt gctaagcgtt atacagatca
     4261 aacctattac actggcgaca acggctatgt cgccggctgg gttgcaggtt acaactttat
     4321 gctgggcagc gagaaattca ctcttaccaa ctggaacgag tacgagtttg accgtgacgc
     4381 tacctatgca gcgggtaatg gcggtaaaga agggctgaat ggcgcagttg cactgtggtg
     4441 gaatgcaaca tcacacatta ctacggggat tcagtatcga tatgcggatg acaaactggg
     4501 cgaagatttt taccaggatg cgataatcta ctccatcaaa tttaacttct gattgattac
     4561 tgccggggct cccggcagta tgaattcact ctatatctga tacaacggct ccccttcccg
     4621 accagccatt agcgcattgc cctggtctcg taccacgtcc cataacgctt cagcggcggt
     4681 ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg cgttcaacaa ccggcgtaat
     4741 gcgctttacc accagtgggc taccttcagg caatggtagc gccagtgcgg gaagaatact
     4801 gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc ggatgtccaa tctcctgcac
     4861 aatattcgcc tgaataccat tgcgcgccag cgctgcgtca atcagcggtc ggctgcctga
     4921 cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc tgccagggta cataatcttc
     4981 gacggccaaa gcgctatcgc gatggcaaag cagaaagaaa ggctcggaaa gaatcgcttc
     5041 acattgcaga tcgcccactg ggcctggatc gatgacgatg ccaaagtcga catctccctg
     5101 gcgaatactt tccatcaccc actgctgcgg acgatcgtgc aggacaaact gaatatctgg
     5161 atagcggcga tggctttcag caatgcattg cggaataaga tgcgcggaaa tggtctggct
     5221 ggcagcgacc cgaacttttc cgctcagttg ttgccccata cgcccggtat cgcgcaacgt
     5281 gctgttcagt tcatccagca gtcgctcaag acgcaaagcc agctgctgcc ctgcatctgt
     5341 aagcaccact tcacgcgtgg ttctgtccag caggcgaaca ccagtatgat tttccagttc
     5401 cttcacactg tgactcactg ccgactggct caggccgata cgctctcctg cacgactaaa
     5461 acttttctcc tgcgctactg tgacgaaaac ctttaattgt tttaaagaat aattcatctg
     5521 ttttcttcat gaatggatgc aataaatcaa ttttatttct caaactggaa gaagcacaat
     5581 agaaccatcg atcatctgga gtctttatga aactttttcg tatcctcgat cctttcacct
     5641 taaccctgat cacggtggtg ttgctggcct ctttctttcc ggccagaggc gatttcgtcc
     5701 ccttctttga aaatctgacc accgcagcga ttgccctgct gttctttatg cacggcgcga
     5761 agttgtcgcg tgaggcgatt attgctggcg gtggtcactg gcgactgcat ttgtgggtaa
     5821 tttgcagcac cttcgtgctg tttccgattc tgggtgtact gtttgcctgg tggaaaccgg
     5881 taaatgtcga cccgatgctc tactccggtt ttctctactt gtgcattctc ccggctaccg
     5941 tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa cgtcgcggcg gcggtttgtt
     6001 ctgcgtcagc atccagcctg ctggggattt tcctttcacc attgctggtt ggtctggtga
     6061 tgaatgttca cggtgcaggg ggcagccttg agcaggtcgg taaaattatg ctgcaactgc
     6121 tgctgccgtt tgtgttgggg catctttccc ggccgtggat tggtgactgg gtgtcgcgca
     6181 ataaaaaatg gattgcgaaa actgaccaga cgtccattct gttggtggtt tatacagcat
     6241 tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg ctggggatca ttgctgttta
     6301 tcgtggtggt cagctgcgtt cttctggcta tcgtgattgt agttaacgtc tttatggcac
     6361 gccgactggg cttcaataag gcagatgaaa ttactatcgt cttttgtggt tcgaaaaaga
     6421 gtctggcaaa tggcatcccg atggcaaaca ttctgttccc cacatcggtg atcggtatga
     6481 tggtgctgcc cctaatgatt ttccatcaga tccaattgat ggtctgtgcg gtgctggcgc
     6541 gtcgatacaa acgccagacc gaacagttac aggcacagca ggaaagcagc gccgataaag
     6601 cttaaagcgg acgcttcagg ggctggacca gttgcgtcag cccctcggtt ttaatcagca
     6661 gcgtgatttg cattagctca ccgagcttcc ctgccgggaa ttcatctttg cgggcaaacc
     6721 acagcagata ctcctccggc aggtcgatta agcgacgccc tttatatttg ccaaacggca
     6781 ttatcgtatt ggctatttca atcagctgct ctttttccat ctcagctacc cagcaaacgc
     6841 agcatttccg cttcgtcgat gacttcaatg cccagttcct gcgctttcgc cagtttagaa
     6901 cctgccgctt ctccggcaat caccagatcg gttttcttcg acacgctgcc cgcgactttc
     6961 gcgcccagtt caaccagtcg agctttagcg tcatcacgcg acatctggct taagctgccc
     7021 gtaagcacca cggttttacc agcaaacggg ctgtcaatct cttccgcgtt gataacgatc
     7081 ggcgcaggcc agtgaacacc ttccgccaac agctcgctga tgacattgcg gttgctttct
     7141 tcggcaaaga agttgtgaac gtgggatgca acgacaatgc caacatcagg caccttttgc
     7201 agctcttcaa tcgaagcggc ttccagcgct tccagcgtgc cgaaatatgc cgccagacct
     7261 gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat agaggaagcg agcaaaggtg
     7321 gtttctttcg ctttttccag cgcgttaacc acgttttgtg ccgattttgg ccccatacgc
     7381 tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat ctgccggagt atggacatat
     7441 tctttttcaa ccagctgatc gatgattttg tcgcccattc cgtcaacatc catcgcacgg
     7501 cgggaaacaa agtgtttcag cgactcttta cgctgcgcac cgcaaatcag gccaccggta
     7561 cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag aaccacatac cggacaatgc
     7621 gtcgggaata caacctcacg ggtatcttcc gggcgttcag aaagcacgac gttaaccacc
     7681 tgcgggatca cgtcgccagc gcggcgaatc accactttat cgccaatgcg taaaccaaga
     7741 cgttcgattt catccgcatt gtgtaaggtt gcgttactca ccagcacgcc agcaacatgg
     7801 acaggttcca gacgcgcaac aggcgtaatc gcgccagtac gcccaacctg aaactcgacg
     7861 tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa acgctaccgc ccagcgcggg
     7921 gcacgcgcga caaagccaag ctgctcctgc tgttccagtg agttgacctt aatcaccacg
     7981 ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca ctttgtgata gaacgccagc
     8041 acttcttccg ccgattcaca aagcgttacc cgatcgctga ccggcaaccc ccacttttta
     8101 aattgcagta aacggccaag atgagtatcc ggcagctcgc caccttccag aacaccaacg
     8161 ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac gcggatcaag ctgacgcagt
     8221 gaaccagctg ccgcattacg tgggttagca aaaactttcc cgcccgtgcg tcgcgcatct
     8281 tcgttaatct tttcgaaccc cgcctgcggc aggaacactt caccacgcac ttccagacgc
     8341 gccgggatat tctctccgtg cagcttcagc ggaatagcgc gaatagtacg cacattagac
     8401 gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg cactgactaa aacgccattt
     8461 tcatacagaa tactgacggc aaggccatcc agcttcagct cacagcacca ggtgactttc
     8521 tcgttgtttt tcagacggtc ctgcacacgt ttgttgaaag caagaaagct ttcttcatca
     8581 aaaacgttat ccagtgacag cattggtact tcatggcgta tctggctgaa agccgccagc
     8641 ggcgcagcgc ctacacgttg ggtaggcgaa tcaggcgtaa tcagttctgg atgtttggtt
     8701 tccagctcgc gcagttcgcg catcagcctg tcgtattcag cgtcgggaat ttccggcgca
     8761 tccatcacat gataaagata ttcatgatgg cgaagcgtcg ttcgcagttc tgtcagttgt
     8821 tgttcgattg attccatatc gcaccatcaa tgctaaaaac ccccgacaag cgggggttcg
     8881 aagaggagtt aatttgcctt aagtgtatca ggcgttggcg tctttgactt cgcggatgat
     8941 gtcctggtac tcgcgcaatt tctgcggagt catcatacgg cgctgatcgt caagcacgac
     9001 accgcccact tcatcggcaa tatgctgcgc agattgcagc atcagcttga agttctgcag
     9061 ctcgtcaccg taagacggta cctgcataaa gatggtgaca cccggagtag tgaaatcctt
     9121 catttcagga tcaaaggttc ccggtttcac catattcgcc aggctgaata acgccgggcc
     9181 gctgccatcc gggctaagat gacgatggta aatattcata tcgccaaaaa tgaagcccgc
     9241 ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg ctaccgtgat gcgccgcgac
     9301 gttcatgata atcaccgctt ctttgcgctt cggtttatcc ataactggag ccggttccgc
     9361 tacaggctca ggctgtggtg ccgctacggg ttctgcaggc tggaaagcct gttgtgccgg
     9421 ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc tgtggcgaaa ctggctgctg
     9481 caacggctgt tcaggctgcg gctgataggc aggctgctgc accggctgcg ctggacgcgg
     9541 agcatgttgc ggcggtacct gcgcttcagg cggctgctgg accggttgac gcggctgcgc
     9601 agacgcataa ggcggttggt actggtgttg cggcgacgga cgagcagcct catgctcctg
     9661 agcgttagcc ggggcatgat tcacgcggtg aacacgaacc tcaccaacgc cctcatcatc
     9721 ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac ttcattcgtt ttaatggccg
     9781 atcgcggaac atagaagatc gttctttacg gctggtccag aaaccatgta ccagtaaagc
     9841 gattatggcg atcgcgccaa caatgattaa tatcagacgc aaatcctgca tcattatatt
     9901 ctctgttgtt ctaacacctt gccaccacgg caaacattta ctcactaaga gtatttgccg
     9961 attacctcaa gtgcaagtgc actattaact ttcacagcac aaagatagat gaaatcgtgc
    10021 tttttgctgt tttttcgaac atatcctaac tgtccattgc gcaattaccc ggtaaaatac
    10081 gcagaatttt cctgggattg gtcaaaagga gctcatcctg attatggttt catcattcac
    10141 atctgcccca cgcagcggtt tttactattt tgcgcaaggc tggaagctcg tctcgcaacc
    10201 tgggattcgg cgtttcgtta ttttaccgct gctggtcaat attttgttga tggggggcgc
    10261 attctggtgg ctctttacac agctcgatgt ctggatcccg actctcatga gttacgttcc
    10321 ggactggcta caatggctga gttatctgtt gtggcctctg gctgtcatct ctgtgctgtt
    10381 agtgtttggc tatttcttct ccacgattgc taactggatt gccgctccgt ttaacggttt
    10441 attggctgaa caactggaag cacgattgac tggcgctaca ccgccagata ccgggatttt
    10501 cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa tggcaaaaat ttgcctggta
    10561 tctgccgcgc gcaattgtat tgctaattct ttacttaatc cctggtattg ggcaaaccgt
    10621 cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta gccatccagt attgcgatta
    10681 ccccttcgat aaccacaaag tgccgtttaa agagatgcgc accgccctgc gcacacgcaa
    10741 aatcaccaat atgcagtttg gtgctttaac cagcctgttt acgatgatcc cgctgcttaa
    10801 tctgttcatc atgcccgttg ccgtttgtgg cgcgacggcg atgtgggtcg attgctatcg
    10861 cgataaacac gcgatgtggc ggtaacaatc taccggttat tttgtaaacc gtttgtgtga
    10921 aacaggggtg gcttatgccg ccccttattc catcttgcat gtcattattt cccttctgta
    10981 tatagatatg ctaaatcctt acttccgcat attctctgag cgggtatgct acctgttgta
    11041 tcccaatttc atacagttaa ggacaggcca tgagtaagat ttttgaagat aactcgctga
    11101 ctatcggtca cacgccgctg gttcgcctga atcgcatcgg taacggacgc attctggcga
    11161 aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg tatcggtgcc aacatgattt
    11221 gggatgccga aaagcgcggc gtgctgaaac caggcgttga actggttgaa ccgaccagcg
    11281 gtaataccgg gattgcactg gcctatgtag ctgccgctcg cggttacaaa ctcaccctga
    11341 ccatgccaga aaccatgagt attgaacgcc gcaagctgct gaaagcgtta ggtgcaaacc
    11401 tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat ccaaaaagca gaagaaattg
    11461 tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt cagcaatccg gcaaaccctg
    11521 aaattcacga aaaaaccacc ggtccggaga tctgggaaga taccgacggt caggttgatg
    11581 tatttattgc tggcgttggg actggcggta cgctgactgg cgtcagccgc tacattaaag
    11641 gcaccaaagg caagaccgat cttatctctg tcgccgttga gccaaccgat tctccagtta
    11701 tcgcccaggc actggctggt gaagagatta aacctggccc gcataaaatt cagggtattg
    11761 gcgctggttt tatcccagct aacctcgatc tcaagctggt cgataaagtc attggcatca
    11821 ccaatgaaga agcgatttct accgcgcgtc gtctgatgga agaagaaggt attcttgcag
    11881 gtatctcttc tggagcagct gttgctgcgg cgttgaaact acaagaagat gaaagcttta
    11941 ccaacaagaa tattgtggtt attctaccgt catcgggtga gcgttattta agcaccgcat
    12001 tgtttgccga tctcttcact gagaaagaac tgcaacagta atgccagctt gttaaaaatg
    12061 cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc ttcaaacttt cgcccctcct
    12121 ggcattgatt cagcctgtcg gaactggtat ttaaccagac taattatttt gatgcgcgaa
    12181 attaatcgtt acaggaaaag ccaaagctga atcgatttta tgatttggtt caattcttcc
    12241 tttagcggca taatgtttaa tgacgtacga aacgtcagcg gtcaacaccc gccagcaatg
    12301 gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac tggcgctaac aatacaggct
    12361 aaagtcgaac cgccaggcta gactttagtt ccacaacact aaacctataa gttggggaaa
    12421 tacaatgttc cagcaagaag ttaccattac cgctccgaac ggtctgcaca cccgccctgc
    12481 tgcccagttt gtaaaagaag ctaagggctt cacttctgaa attactgtga cttccaacgg
    12541 caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact ctgggcctga ctcaaggtac
    12601 cgttgtgact atctccgcag aaggcgaaga cgagcagaaa gcggttgaac atctggttaa
    12661 actgatggcg gaactcgagt aatttcccgg gttcttttaa aaatcagtca caagtaaggt
    12721 agggttatga tttcaggcat tttagcatcc ccgggtatcg ctttcggtaa agctctgctt
    12781 ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt ctgccgacca ggttgatcag
    12841 gaagttgaac gttttctgag cggtcgtgcc aaggcatcag cccagctgga aacgatcaaa
    12901 acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca tctttgaagg gcatattatg
    12961 ctgctcgaag atgaggagct ggagcaggaa atcatagccc tgattaaaga taagcacatg
    13021 acagctgacg cagctgctca tgaagttatc gaaggtcagg cttctgccct ggaagagctg
    13081 gatgatgaat acctgaaaga acgtgcggct gacgtacgtg atatcggtaa gcgcctgctg
    13141 cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca ttcaggatga agtcattctg
    13201 gttgccgctg acctgacgcc gtccgaaacc gcacagctga acctgaagaa ggtgctgggt
    13261 ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta tcatggcgcg ttctctggaa
    13321 ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg tgaaaaatga cgactatctg
    13381 attctggatg ccgtaaataa tcaggtttac gtcaatccaa ccaacgaagt tattgataaa
    13441 atgcgcgctg ttcaggagca agtggcttct gaaaaagcag agcttgctaa actgaaagat
    13501 ctgccagcta ttacgctgga cggtcaccag gtagaagtgt gcgctaacat tggtacggtt
    13561 cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg ttggtctgta tcgtactgag
    13621 ttcctgttca tggaccgcga cgcgctgccc actgaagaag aacagtttgc tgcttacaaa
    13681 gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc gtaccatgga catcggcggc
    13741 gacaaagagc tgccatacat gaacttcccg aaagaagaga acccgttcct cggctggcgc
    13801 gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg atcagctccg cgctatcctg
    13861 cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga tgatcatctc tgttgaagaa
    13921 gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg aactgcgcga cgaaggtaaa
    13981 gcgtttgacg agtcaattga aatcggcgta atggtggaaa caccggctgc cgcaacaatt
    14041 gcacgtcatt tagccaaaga agttgatttc tttagtatcg gcaccaatga tttaacgcag
    14101 tacactctgg cagttgaccg tggtaatgat atgatttcac acctttacca accaatgtca
    14161 ccgtccgtgc tgaacttgat caagcaagtt attgatgctt ctcatgctga aggcaaatgg
    14221 actggcatgt gtggtgagct tgctggcgat gaacgtgcta cacttctgtt gctggggatg
    14281 ggtctggacg aattctctat gagcgccatt tctatcccgc gcattaagaa gattatccgt
    14341 aacacgaact tcgaagatgc gaaggtgtta gcagagcagg ctcttgctca accgacaacg
    14401 gacgagttaa tgacgctggt taacaagttc attgaagaaa aaacaatctg ctaatccacg
    14461 agatgcggcc caatttactg cttaggagaa gatcatgggt ttgttcgata aactgaaatc
    14521 tctggtttcc gacgacaaga aggataccgg aactattgag atcattgctc cgctctctgg
    14581 cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt gcggaaaaaa tcgttggtga
    14641 tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg cctgtagacg gtaccattgg
    14701 taaaatcttt gaaaccaatc acgcgttctc tatcgaatct gatagcggcg ttgaactgtt
    14761 cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa ggcttcaagc gtattgctga
    14821 agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa tttgatctgc cgctactgga
    14881 agagaaagcc aagtctaccc tgactccggt tgttatctcc aacatggacg aaatcaaaga
    14941 actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc ccggttatcc gcatcaagaa
    15001 gtaattcttg ccgcagtgaa aaatggcgcc catcggcgcc attttttatg cttccgccag
    15061 cggcggcaaa atcaattcat cgctctcatg ctgctgggtg tagcgcatca cttccagtac
    15121 gcgcaacccc gcacggtgca ccgcatcgtt taacgccttc cctttcagca aaccactgat
    15181 aagctgagca caaaacaggt cgccagtccc tttcaggtcg gtttttaccc gtgaatggga
    15241 aatgacattc acgctgtcgg cactgaccac caccacctgc atctcctgat tttcttcatt
    15301 accggaggcg ctggtaatca ccacccattt taatgtgtct gaaagcagac tttttgcggc
    15361 agcaatggca ctgtcgagat cgcggcaatt tttaccggtc aggatttcca gctcaaagat
    15421 attgggggta attccctgcg ccagcggcag taaatattgt cgatacgcct cgggaaggtc
    15481 aggtttgaca taaattccgc tatcaatatc gccaatcacc ggatcgacca tgatcaatag
    15541 gtcaggatgg tctttgcgta gcgcagtcag ccactcggca aggattttga tttgcgatgc
    15601 cgttcccata tagcccgtgg ttacagcgcg cagctggcgc agcgcatcac gctcctgaag
    15661 cgcacgcaaa taaccgctaa accattcatc cggaatcgca ccaccgtaga aagtgtcata
    15721 atgcggcgta ttgctcagca ataccgtcgg cacggcaaaa acattcagac cgttctgttt
    15781 gatagcaggc acggcaatgc tgttgcccac gctgccgtaa accacctgcg actgcacggc
    15841 gacgatatcc gcctgcagcg ccctgctctt atcgttaaac aacaacaaac tactcattta
    15901 attttttctc cttgccgatg atcctcatcg tattccaacc gaaactttac ctgattctgg
    15961 cagtcaaatc ggctatcaca aaacaaggat aaggtaattc aatgaagaaa atcatttgtc
    16021 tggtcattac actactaatg acactccccg cttacgcgaa gttaactgcc catgaagaag
    16081 cccgcatcaa cgccatgctg gagggattag cacagaaaaa ggatttgata tttgtgcgca
    16141 acggtgatga acatacctgc gatgaagcgg tttctcatct gcgtctgaag ctgggcaata
    16201 cccgtaaccg cattgacact gccgagcagt ttattgataa ggttgcttcg tcgtcatcga
    16261 ttaccgggaa gccgtatatt gtgaagatcc ccggtaagag cgatgagaac gcacagcctt
    16321 ttttacatgc gctaattgcg cagacggata aaacggtgcc tgcgcaataa tcttaaatcc
    16381 ccgccccctg gctaaaatgc tcttccccaa acaccccggt ggaaaggtag cgatcgccac
    16441 gatcgcagat gatcgctacc accaccgcac cagggttagc ttttgccacc cgcagtgctc
    16501 cggcaaccgc gccgccggag ctgacgccac agaatattcc ttcccgcact gccagttcgc
    16561 gcatggtgtt ttctgcatcg cgctgatgaa tatccagcac ctcatccacc agagaagcgt
    16621 tgaaaatccc cggcagatat tccgcaggcc agcggcgaat gccgggaata ctgctgcctt
    16681 cttccggctg caggccaaca atcgttactg gtttggattg ttcgcgcata aagcgtgaaa
    16741 cgccggtgat agtgccggtt gttcccatgc tggagacaaa atgggtaatg cgcccgccgg
    16801 tttgctgcca gatttccggc ccggtagtgg tgtaatgggc gtaagggtta tcgggattat
    16861 taaactgatc gagcagcttt ccttcgccac gattcgccat ctccagcgcc agatcgcgcg
    16921 caccttccat gccctgctct ttggtgacaa gaatcagttc cgcaccataa gcacgcatcg
    16981 ccgcacggcg ttcctggctc atgttgtcgg gcatcagcaa tttcatgcga tagcctttca
    17041 gcgcggcaat cattgccagc gcaatgccgg tgttaccact ggtggcttcg attaagacat
    17101 cacccggttt aatttccccg cgcttttccg cctcgacgat catcgaaagt gccgcacgat
    17161 ctttcaccga acctgccggg ttattgcctt ccagttttaa ccacacttca ctgccgttat
    17221 ccggccccat tcgctgcaac ttcaccagag gcgtattgcc tattgtttgt tctaatgtac
    17281 tcacgatctc tatccatacg tggtgttgcc tgatgcgacg cttgcgcgtc ttatcaggtc
    17341 tacaggttac aaaccttgcc attaaaaagc ccggatcgcg ggaagcgcct ccgggcgttt
    17401 aacattcact caacctatca ggcgctttgt gcgagagcaa gttcctcatc tcgggtttcg
    17461 atacgctcgt cgccgttata cagccgcgca tgttgcagac caacgaataa acgctcgcca
    17521 cgctgcgggg catcgtcgcc atgcatcacg accgtcagcg gttcgttgta ccaccccagc
    17581 ggctgcacca ctaattgggt gtagtgacct ttcgggctgg cttccagtac ctgtaccggc
    17641 agcggcgaat cgagactggt acggcggctg atatccactt cccacgggcg caggaagaga
    17701 tccaccggac cctgatacgc aggtgtgtag cccagcggcc agcgatgcgc gccaacatgg
    17761 aactgcccgc cgcgaatggt tccctgcagg cggttcactt cgcccataaa ttcgagtaca
    17821 aaacgggtcg ctggttcgcg ccatacctga ttcggcgcat cagcctgttc aatattcccc
    17881 tgactcatca ccactacacg atcggctact tcggtcgcct cttcctggtc gtgggtcacg
    17941 aacacgctgg tgaatttcag ctcttcatgc agttgacgca gccagcgacg cagctcttta
    18001 cgcacctgcg catccagcgc gccaaacggt tcatcaagca gcagaatttg cggttctaca
    18061 gccagcgcgc gcgccagcgc cacgcgctgt ttctggccgc cggaaagctg tgccggataa
    18121 cgatctgcca gatgagcaag ctgcaccatc tccagcaatt ttgtcacttt cgctttgatg
    18181 gctgcggcat tcgggcgctc gcgacgtggc agcaccgtca ggccaaaagc gatattgtcg
    18241 aacaccgtca tatgacggaa cagcgcgtaa tgctggaaca cgaaaccgac cttgcgatcg
    18301 cgcgcgtgca ggcggctgac gtcggtgccg tggaagcgaa tatgcccgct ggtttgatgc
    18361 tccagcccgg cgataatgcg cagcagcgtg gttttcccgg aaccggacgg ccccagcaac
    18421 gcgaccatct gacctgaagg aatatccagt gagatatcgt tcagcacctg ggtgcgacca
    18481 aacgacttct taatattggc aatctcaatg ctcatgatgt tcctcctgct gtgcgcgttt
    18541 atcctgattc tccaggcgcc actgcaacat actttttaaa aacagggtga taatcgccat
    18601 cagcgttaac agtgccgcag cggtaaagga gccgacggtg ttgtagtcct gctccagcaa
    18661 ttcaatctgt aacggcagcg acagggtttc gccgcgaatc gagccggaaa ccaccgacac
    18721 cgcgccaaac tcgccaattg cgcgggcgtt ggtcaacacc acgccataaa gcagcgccca
    18781 gcgaatgttc ggcaatgtga cgcggcgaaa catctgccag ccagacgcgc caagcaaaat
    18841 cgccgcttcg tcttcctggc tgccctggct taacatcacc ggcaccagct cgcgcaccac
    18901 aaacggacac gtcacaaaga tggtgaccag caccattccg ggccaggaga acataatttg
    18961 cagattatgt tcatccagcc agccgcccag cgggccgtta gagccgtaga acagcagata
    19021 caccagaccg gcaaccaccg gcgatacggc aaacggaatg tccagtagcg tcagcagtaa
    19081 ctggcgtcca gggaagttaa agcgcgtcac cagccaggcc agcagaatgc cgaacaccag
    19141 gtttaccggt acggcaatca gcgcgatcat caccgtcagc cagatggcgt gcagcatgtc
    19201 cggatcggcc agattctgta aaaccggcat cagccccttg ctgaatgcct gcacgaagat
    19261 gtaaatcatc ggcaccagca ggatgaacgc tgaaaccagc atcccgatgc caattagaaa
    19321 ccatttgccc cagttaatcg ggcgcgcgtc ataacgcttc aattgggtaa cttccgccat
    19381 taatgaccta ccacacgccg accaaagcga ctttgcagag tgttaattga gaacagcagc
    19441 agcagggatg ccgcgaggat caccgaagca atcgcgctcg ccgccgggta atcaaactcc
    19501 tgcaagcgca caaaaatcat cagcgacgtc acttccgtct tccaggcgat gttcccggcg
    19561 ataaatatca ccgcgccaaa ctcaccaaga ctacgggtaa acgacaacgc cacgcccgcc
    19621 accagcgctg gagaaagttc cggcagcacc actttgcaga aactctgcca gcgcgttgca
    19681 ccaagcgttt ccgccgcttc ttcatattcc gggcctaact cttccagcac cggctgcacg
    19741 gtacgcacca caaacggaat gctggtaaag gccatcgcca ccgcaatacc aagccaggta
    19801 taggtgactt tgatatcaaa cttcgccagc cattcaccgt aaaaaccgtt tacggaaaag
    19861 agcgaggcca gcgttaaacc ggcgactgcc gttggcagcg caaagggtaa atccatcagc
    19921 gcatcaagca gcgtgcggcc cggaaagcga taacgtgtta aaatccacgc catcagcaga
    19981 ccgaaaacgc cgttaaaaat cgatgccaca aacgccgaca gcagcgtcac tttgtaggcc
    20041 gcgaccactt gtgggttggt gatcacctcc cagtactgcg cccagctcat ctgagccagc
    20101 tgcatcacca gcgcggagag cggcagcagc aaaatcaggc acacaaacag cagactggtg
    20161 ccgaggctta aggtaaagcc cggcagcacg cgtcttgatg aaactgcaaa catcagttac
    20221 gccccgccgc taacagcttg tctaactcgc cgccgctggt gaagtgggtt ttcatcactt
    20281 ccggccagga gccaaatttg tcttccacgc ggaacagctc ggtctgcggg aatttatctt
    20341 tcagtttgtc catgacttcc gggttattta cgcggtagta atagtcggtg atgatggtct
    20401 gcgcctgcgg gctgtagagc cagttcaggt aggcttttgc tgctttttcc gtaccgttgg
    20461 cctgcacgtt tttatcgacc cacgccaccg ggaattccgc cagaatattg gttttcggga
    20521 tcaccacttc aaagccctgt gcttcatact gtttacggat gttgttcact tccgattcaa
    20581 agctaatcag cacatcgccc aggccacgct cggcaaaagt ggtggttgcg ccacgaccgc
    20641 cggtatcgaa cacttcaaca tttttcagga actgggtcat aaactgttcg gttttggctt
    20701 tatcgccacc gtcagcttta tccgctgcgc cccatgccgc cagataggta taacgcgcgt
    20761 tacccgacgt tttcgggttc gggaaaatca gcttcacgtc ggagcgcacc aggtcgttcc
    20821 agtcgtggat attcttcggg ttgcccttac gcaccaggaa gcccatggtg gagtagaacg
    20881 gcgagctatt attcggcagg cgcgactgcc agtccgccgg gatcagcttg cctttgtcat
    20941 gcaggatttg tacgtcagtc acctggttat aagtgacaac gtcggctttt aagccctgta
    21001 aaatcgccag cgcctgtttt gatgacccgg catgagattg tttaatcgtc agtttgtcgc
    21061 cgccgttatc tttggcccat tgctgttcaa acggcgggtt cagggcggca aacagctcgc
    21121 gggagacgtc ataagaactg ttcagcagtt ccgttgcctg tacatggcct gccagcagca
    21181 gagaagcgac cagcgcgagt gagttctttt tcagtaagtt aacggccatt gcgcaccctt
    21241 atatatttaa tgactttcta atagccatca tatttataac ggacgtgaaa ggagtaacgg
    21301 ttttatatac cgtttggtga tttggaagtt gaaaagggaa taagaaccgg gtttaaaacg
    21361 aaatcctccc cacacatgca gggaggaaac agaggtgaat cagataccga cgctaaccgt
    21421 ctccggcagt gtgctgccgc catcaatcac attctgtgta ccggttaaat agctggattc
    21481 atccgatgcg aggaaggccg ccagttcgcc gacttccagc ggatcggcga ggcgacgcag
    21541 cgggattgct tttgccatat cagtcagcac cgactctgga tcttccgggt tcgactggcg
    21601 ggcaatgctt tccgccattg gcgtgcggac gtaccccggg cagatggcgt taacgcgaat
    21661 accggattgc gcgtactcca ccgccagcga ttttgtcagg ccaacaatcg ccgctttcgt
    21721 taaggcgtac gccgtttcac ccggatcggc caccatatca ccagtgactg aagacatcat
    21781 cacaatgcga ccatctttgc gcgcaatcat ctctggcagc accgccttcg tgacgttcca
    21841 tacgccttta atattgatgt caatatggaa atcgcgatct tcatcgctca tatcgaggaa
    21901 actgcccaga cgacaaacgc ctgcgttatt caccaggata tcaatgcgcc cttctttttc
    21961 cttcgcgcgt ttgatagctg cggctaccga cgccgggtca cgcacatcgg cgacaaccgc
    22021 cgtacagcga tgaccacgac cacacagttc gtccgccagc ttttcgatct caggggagat
    22081 atccagcaag attaggtttg cgccatgacg cgcaaaagtt ctggcaattc cttcgccaat
    22141 tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg agtttaccca ttttaatgct
    22201 ctccttgtta atacagagcg ttacactcca ttccctctga aaatacagga taaacaaaat
    22261 attgctgcgg atcagctcac taattcagga aaatctcaat ccagtcgctg ctttatgcat
    22321 catttcaggc gttgtgttag ctcactgctg cgctgaatca ttttcaatga ctcgacatca
    22381 ttgagttgca caagaccaac aaacagtaaa tcggtaacag aattttgcgc cgtgcgggta
    22441 gacatcgacg agctacgcca ttcggtttca ccggaaacgg tatcgagcgt gaaatgcgcc
    22501 agccgccgca gcggagagtc agccagagaa gtaatggcaa tcaccgttgc cccctgcttt
    22561 cgtgccgctt ctgcgcacaa gacaatctct tttttactgc cgctgtaaga gatagcgatt
    22621 tgtacatcgc cttttttcaa cgcctgggaa acggtggctt gtacgtgcgt gtcggcttcg
    22681 caggccacgc gatatccaat tttcatcagt ttgaacgaca gatcgcgccc caccagcgcc
    22741 gatccgccca ggcccgttat ctgaataaat tgcgctttac tgatgacatc aatgattttt
    22801 tgcagccgcg cgtagtcgaa caacgcgcag gtttgctcca gcgccagctc tttttcgcga
    22861 ttcagtttgc gggcaatcac ttccagcgaa tcatcactgg taatcgaact gtgcaggtgc
    22921 agcgccgtgg cattcgtttt ctcccggctg gcactgtatt cgccaattaa cgccatacgc
    22981 aattcagtaa atccttgtgc gccgagcttc tgggcaaact tcacaatact cgactggctg
    23041 atgccgagct gtttcgccat cttgcgcgaa gagacggatt gcagctcact cacgttggcc
    23101 tgcagaaagt cagcgatttt ttgctcgttt tcggtgaatt cactccccgc attgcttatt
    23161 ttcgtcaggt acaacatctt ccactctcct gggctggcag cataaattcc gcttaagttc
    23221 ttatgacgct cttcacactc tgcgagtgta gactcaatga ttcctttaag tctgtcaatt
    23281 cgggatatat gattccatac atctgcaatt atttgatcgt aaatagtaag gtcaccaccg
    23341 atgcaactag aaaagatgat tactgaaggc tcgaacaccg cctcggctga aattgaccgc
    23401 gtatcgacgc tggaaatgtg ccggattatc aacgatgaag ataaaaccgt accgcttgcc
    23461 gttgagcgcg tcctgccgga tatcgccgcg gcgatcgatg ttatccacgc ccaggtcagc
    23521 ggcggcggtc gtctgattta tctcggtgcg ggaacatccg gtcgtctggg gattctggat
    23581 gccagcgaat gtccgcccac ctacggcgtg aaaccgggtc tggtggttgg tttgattgct
    23641 ggaggcgaat atgccattca gcacgcggtg gaaggcgctg aagatagccg ggaaggtggt
    23701 gttaacgatc tgaaaaatat taatttaacg gcacaggatg tggtggttgg cattgctgcc
    23761 agcggtcgca cgccgtatgt gattgccgga ctggaatacg cacgccagct cggctgccgc
    23821 acagtgggaa tttcctgtaa tccggggagt gccgtttcca ccaccgccga gtttgccatt
    23881 acaccgattg taggtgccga agtggtcact ggttcttcac gaatgaaagc aggcacagcg
    23941 cagaaactgg tgctcaatat gctttccacc gggctgatga ttaaatctgg caaagtgttc
    24001 ggcaacctga tggtcgatgt ggtcgccacc aacgaaaaac tgcatgtgcg ccaggtcaat
    24061 atcgttaaaa acgccaccgg atgtaatgcc gaacaagcgg aagcggcgtt aattgcctgc
    24121 gagcgcaact gtaaaacggc cattgtgatg gtgctgaaaa atctcgatgc cgcagaagct
    24181 aaaaaacgcc tggatcaaca cggcggcttt attcgtcagg ttttagacaa ggaataaccc
    24241 atggccaaag agatcagcag tgaacttctg aacaccattc ttacccgtgt cggcggaccg
    24301 ggaaatatcg ccagttgtgg taactgtatg acgcgcctgc gtctgggtgt acatgacagt
    24361 tcactggttg atcccaatat caaaacgctg gaaggcgtga agggcgtcat tttgaccagc
    24421 gaccaggtgc aggtcgtttt tgggcctggt aaagcgcatc gtgccgcaaa agcgatgagc
    24481 gagctgctgg gcgaggcacc ggtacaggat gccgcagaaa tcgccgccca gaacaaacgt
    24541 cagttaaaag ccaaacaaac ctccggcgtg caacaatttc tcgccaaatt cgccaccatc
    24601 ttcacgccgc tgatccccgg ttttattgcc gccggtctgc tgctggggat agcgacgtta
    24661 attgccacgg tgatgcacgt tccggcagat gctcagggca cgctacccga cgcgctgaat
    24721 tttatgaagg tgttcagcaa aggtttgttc actttcctgg tgattctggt gggctataac
    24781 gccgcccagg catttggcgg cacgggcgtc aatggcgcaa ttattgccgc actctttttg
    24841 ctcggttata accccgctgc aaccaccggt tactacgccg gttttcacga tttctttggt
    24901 ctgcccatcg atccgcgcgg caatattatc ggcgtgctga ttgccgcgtg ggcctgcgcc
    24961 cgcattgaag gcatggtgcg ccgctttatg ccggacgatc tcgacatgct gctgacctcg
    25021 ttaatcaccc tgctgatcac cgctacgctt gcgtacctga tcattatgcc gctgggtggc
    25081 tggttattcg aaggtatgtc atggctgttt atgcacctga acagtaatcc gttcggttgt
    25141 gcggttttag ccgggctgtt cctgatcgcc gtggtatttg gcgtgcatca gggctttatt
    25201 cctgtttacc tcgcgttaat ggacagccag ggattcaaca gcttatttcc catcctttca
    25261 atggcaggcg cgggccaggt gggcgcggcg ctggcactct actggcgggc gcaaccgcac
    25321 agtgcattac gcagtcaggt acgcggggcg attattcccg gtctgctggg cgttggtgaa
    25381 ccgttgattt acggcgtcac cctgccccgc atgaaaccgt ttattaccgc ctgtttaggc
    25441 ggcgcagcgg gcggtttgtt tatcggcctg atagcctggt ggggtctgcc gatgggctta
    25501 aacagcgcct ttgggccgtc tggtctggtg gcgctgccgc tgatgacttc cgcacaaggc
    25561 atccttccgg caatggcggt ttatgctggc gggattctgg tggcatgggt ttgcgggttt
    25621 attttcacca cgctctttgg ctgccgtaac gtcaatctgg actgatatca tgaaacggac
    25681 aatgctctat ctttctctgc tggctgttag ttgtagcgtc agcgccgcaa aataccctgt
    25741 tctgacagaa agttcgccag agaaagcagg gttcaacgtc gaacggctta accagatgga
    25801 tcgctggatt agccagcaag tggacgccgg ttatcccggc gtaaacctgc tgatcattaa
    25861 agataatcag attgtgtatc gcaaagcctg gggagcggcg aaaaagtacg atggcagcgt
    25921 gttgatggaa cagcctgtca aagccaccac cgggacgctg tatgacctgg cctcaaacac
    25981 caaaatgtac gccacgaact tcgccctgca aaagctgatg agcgaaggca aactgcatcc
    26041 tgacgatcgg attgcgaaat atattccggg gtttgccgat agtccaaatg acaccatcaa
    26101 aggcaaaaac acgctgcgga tttctgacct gctgcatcac agcggcggtt tcccggcgga
    26161 cccacaatat ccgaataaag cggtcgcggg cgcgttatat tcccaggata aaggccagac
    26221 gctggagatg atcaagcgca cgccgctgga gtatcagccc ggcagcaagc atatttacag
    26281 tgatgtcgat tatatgctgc ttggatttat cgtcgagtcg gtcaccggtc agccgcttga
    26341 ccgctatgtt gaagagtcga tttatcgccc gctcggcctg acacatacgg tatttaaccc
    26401 gctgctgaaa ggctttaaac cgcagcaaat tgccgccacg gaattgaacg gcaatacccg
    26461 cgatggcgtg atccatttcc cgaatatccg cacctccact ctctggggtc aggtgcacga
    26521 tgaaaaagcc ttttattcga tgggcggcgt ttccgggcac gcaggtttgt tttccaatac
    26581 cggcgatatt gcggtgttaa tgcaaacgat gctgaacggc gggggctatg gcgatgtgca
    26641 gctgttcagt gcggaaacgg tgaagatgtt caccaccagc tctaaggaag atgccacttt
    26701 tggcctcggc tggcgcgtga atggtaatgc caccatgacg ccgacgtttg gcacgctggc
    26761 aagcccgcaa acctacggtc acactggctg gaccggaacg gtgaccgtta tcgatccggt
    26821 gaatcatatg gcgattgtga tgttaagcaa caagccacat tcgccggttg ccgatccgca
    26881 aaagaatccc aatatgttcg aaagcggtca gctgccgatt gcaacgtatg gttgggtagt
    26941 tgatcaggtg tatgcggcgt taaagcaaaa gtaataaaaa ggccagtcag taatgttact
    27001 gcctggcctt taatcaatga atcagaaacg cttacagcgc catcaacttg tccagcgacg
    27061 gtgcgaaata atagccgccg gttaccggtt tggtgaaacg caacatcgca tcacgcttac
    27121 catcggtatc gccaaacatg ctcagcagtt gctgctcaat gttatgcaga cgcgcgcagt
    27181 aggcgcagaa gtacagaccg tgagtgccac tggcagtgcc gtacggcagg ctctggcgaa
    27241 caatcttcag ccctttgcca tcttctttca gatcaacgcg ggtgaggtga gaggtttccg
    27301 gacgttcgtc gccgtcgatc tcttcgttgg cctctttggt gcgcccgatc atcatctcct
    27361 gatcgtgaac gctcatccgg ttgagctgct tcaggttgtg ttcccaacgc tggacaaaca
    27421 catagctgcc gcccgcatcc acgccgtctt tgataaccgc cacttcgcga cgcgtctctt
    27481 cacccgccgg gttttccgta ccgtcaacaa agccgctcag gtcacgctct tcaacccaac
    27541 ggaagccgtg gatctcttct ttcacttcaa tgcagtcacc aaaggcttcc atcgccgcct
    27601 gggcgacaga gaagtttacg tcgtgacgca gagaaagaat gtggatcaac acatcgaact
    27661 gggtggtcgg cgcaaggcct ttaccgtagc ccggaaaatc tttcagctct tctgccccaa
    27721 cgccgccgct cagagcgcgc caggtgttgt taccaaaggc aaccaccgca ccaagatgcg
    27781 cgtccgggaa tttcgcttca aaagttgcca gtttgtcggc aaatgtttta ctggccgcac
    27841 gcagggcgtc aacttcccct ttcacgttgg cttcgatcca aatcgccgcg cggcaatgtt
    27901 ctggcaaaat gccactctga acctgagaca ttgttcctcc tgaaaataat aatgccacgc
    27961 aatccgtggc attgttgagc gctattttac gtgttttttc gcggcggcgg tttgttcaga
    28021 cgcaaattaa cgccgccaga taattttact gactttccag tttttcaggg tatcgtccga
    28081 aggcattaac ccttcaggac cactccattc cccgctgaac tggtagctaa tatgttgact
    28141 gccttcggct ttacattcga cggcacgatt atcatcacca tcagcttttt ggcaattgcc
    28201 aaatgctttg ctgtaaaggt cgctaaacgg tgtaccgatt ttaacaccgg tgtcagcagg
    28261 aatatcgcta tccagcacgt caatgcgact gatcgtgcct tgatcgccgt taatcaccat
    28321 cgccacgttg tcgcccttca tcacttcaaa aaagcgcacc acgttgccgt tcgcggtttt
    28381 cattccgctg cgcaggcgat aatcgccatc aagcgcatcg gcaatggctt gttcttgcag
    28441 tggtgtggac gccgttaatt caccaacgcc ctgctcgctc actttggtgg acgacccaaa
    28501 ccagttccac gggttagcgg ccgaccagtt aactgacgac atcgtggaac agccggtcag
    28561 catcaacggc atagcgcata acattaaacg cagcgatttc atgtcacttc ctttggttat
    28621 tcaataacgt tgcttggagt gcaaattcac caaaaagtgc cgttattctt tttcttcatt
    28681 aaaacaagcg cgtaaacgtc gattggtcag cagccagatt aacgccacaa tatccgccac
    28741 taccagcgcc agaccgatac cgctaacgga ttcaccgttc agccacagcc acggttgcca
    28801 gcaaagtaaa accacctgcg ccaacagcaa cagaaaatag agcacatgcc aagtgcgagg
    28861 aaatgtagcc cgccgaccgc tcaacaaaaa cgccagcacc gccggaatgc ctggaatcag
    28921 ccccagccag aaattatcgt gatcgggata aaacagattt agcagcgcag taccctgctc
    28981 gcgcgacgca ccggcaatga caaacagcac ccaggttcgc gcctgaagca atagcacaag
    29041 ccagaagagc aagggtaaac gcaggcgtcc gtgcgcatca taatggacag gatggaactc
    29101 agtactcttc atcttcaatc aaacgcttac ccagactcag cacgtcggcg tgttcatatc
    29161 ccaggcgttc atacattccg agcaccatgt cgttatcttc cggcacattg atctgaattt
    29221 tcgggcagcc acgagcaatc agctttttct ccagccgatt aagcaacgca ttggcaatcc
    29281 cacgcccacg aaactctgga tgcacgccaa gataataagc agacccgcga tgcccgtcat
    29341 aaccgcccat caccgttccg accacttcac cgtttacctc agcgaccaaa aacaaactga
    29401 cgtcatggtt catcttacgc tcgatgtcca tttccggatc gttccacgga cgcagcaagt
    29461 cgcaacgctc ccaaagggtg atgacctctt cgaaatcttc ctggcgaaat acgcgtatct
    29521 ccatggtatt cgttaccttt ttgcgggtta aaaggctgat tatggcgtga acggtcgaat
    29581 tagccaatat ctgacgaaaa tcggttgaaa aagtggcata atggggagtt gtcaactatt
    29641 gaaatgaaaa gtaaaacaat tctcaacatc aaaccgtcgt aacggattac gcgatacgat
    29701 ataacatctg gaactttatt attacaactc aggccgtatg agcactttta aaccactaaa
    29761 aacactcact tcgcgccgcc aggtgctgaa agccggtttg gctgccctga cgttgtcagg
    29821 aatgtcgcaa gccatcgcca aagacgaacc tttaaaaacc agcaacggac acagcaagcc
    29881 gaaagccaaa aaatctggcg gcaaacgtgt cgttgttctc gatccaggcc acggcggaat
    29941 tgataccgga gcgatcggac gcaacggttc gaaagaaaaa catgtggtgc tggcgattgc
    30001 taaaaacgtc cgttccattt tgcgtaatca tgggattgat gcgcgtttaa cgcgttctgg
    30061 cgatacgttt atcccacttt acgatcgcgt tgaaatcgcc cataaacatg gcgcagatct
    30121 gtttatgtca attcatgccg atggctttac caacccgaaa gctgccggtg cttcggtatt
    30181 tgccctctct aaccgtgggg caagtagcgc aatggcgaaa tacctgtctg aacgcgaaaa
    30241 ccgcgccgat gaagttgccg gtaaaaaggc gactgacaag gatcacctat tgcagcaagt
    30301 gctgtttgat ctggtgcaaa ccgatactat taaaaacagc ctgacgctcg gctcgcatat
    30361 tctgaaaaag attaagccgg tgcataaact gcacagccgc aacaccgaac aagccgcgtt
    30421 tgtggtgttg aaatcgccgt cggttccttc ggtgctggtg gaaacctcgt ttatcaccaa
    30481 cccggaagaa gagcggctgt taggcacggc ggcgtttcgt cagaaaatcg ccacagcgat
    30541 tgctgaaggc gtgatcagtt atttccactg gttcgacaac cagaaagcac attcgaaaaa
    30601 gcgataagtt atgaaacccg acgcacacca ggttaaacag tttctgctca accttcagga
    30661 tacgatttgt cagcagctga ccgccgtcga tggcgcagaa tttgtcgaag atagttggca
    30721 gcgcgaagct ggcggcggcg ggcgtagtcg ggtgttgcgt aatggtggtg ttttcgaaca
    30781 ggcaggcgtc aacttttctc acgtccacgg cgaggcgatg ccagcttccg ccaccgctca
    30841 tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc gtttcactgg tagtgcatcc
    30901 gcataacccg tatgttccca ccagccacgc gaatgtgcgt ttttttattg ccgaaaaacc
    30961 gggtgcagag cccgtctggt ggtttggcgg cggcttcgat ttaacccctt tctatggttt
    31021 tgaagaagac gccattcact ggcaccgcac cgcccgtgac ctgtgcctgc catttggtga
    31081 agacgtttat ccccgttaca aaaagtggtg cgacgattac ttctacctca aacatcgcaa
    31141 cgaacagcgc ggtattggcg ggctgttctt tgatgatctg aacacgccag atttcgacca
    31201 ctgttttgcc tttatgcagg cggtaggcaa aggctacacc gacgcttatt taccaattgt
    31261 agagcgacgt aaagcgatgg cctacggcga gcgcgagcgc aattttcagc tctaccgtcg
    31321 cggtcgttat gtcgagttca atctggtctg ggatcgcggc actctgtttg gcctgcaaac
    31381 tggcgggcgc accgagtcga tcctgatgtc aatgccgcca ctggtacgct gggagtatga
    31441 ttatcagcca aaagatggca gcccagaagc ggcgttaagt gagtttatta aggtcaggga
    31501 ttgggtgtaa ctccctcacc cccattcccg catccgctga tgcaacgtca acgacggctt
    31561 ctcggcaaac agctgctggt aatccgtggc aaattgcccc agatgccaga atccccactg
    31621 catggcggcg tcttttaccg ttgtgctttg cgaccacggg cttatcagtt cgcggcgtac
    31681 ggcgttcagg cgaatgcgtt tcagccacgc gtttgggcca atgcctaaaa tagcgtgaaa
    31741 cgcgttttgt agcgtgcggc gactgacatg cagttgatta cacaagtcca gcaccgtcac
    31801 cggctcagac atattttcca gcacatattc acgggcgcgg gaaagtaatc tacggtaact
    31861 ctgatgactg atgctttccg ccgtcaccat tggttgcgcg tcttccagca tcgcccccat
    31921 cgccattagc aaattatccc ccagcacttt tcgcactgca ggctgatgga gattttccgg
    31981 gttctcgcaa aacgtcgcca gcgcctgttg gacaaagccc cacagcgcgg ctttatgctg
    32041 ctctttcact tccagcgccg actggctacg cagcatatgt aatacccgat ccgggttatg
    32101 caaaaagtta gcctgccggg tgatgacatc ttcagaaagc accacgccca ggatcgtgta
    32161 atcatccggc gtgctcagtt caaattcagt gccaccaggg cgggtggcga tttccgcgct
    32221 tcccagacat tgcgaaccga taaatccctg ctcaccgcgc gtcgccggaa tgccaaacca
    32281 gaacgagttc ggccagacca ggcacgactg acgcagcgcc agaccggtgt attcacgaaa
    32341 aacctgaata tcatcgagta gaatttccgt aaattcacaa tgaaacttgc ccggatgcag
    32401 ctgatcgtaa atctgctgcc aggcggtaat cgttaaagca tgttcataga catccgttgt
    32461 ctgtcgttga tgaacattat ccacttcgac cttcggcgtg agcttcaggt tttcgggtaa
    32521 gggttcatga taaagatggt gcaaattggc tgtacgggtc tttttcatga tgttaatgcc
    32581 gggtgttgta ggacacccgg cacctccgac aggttaatgg ggcttgagac gataacgact
    32641 actgcgttta cgtaacgtcc cggcagaaaa gagctgttcg agcgcatttc tggctttttc
    32701 cagcggccag ccaaagtggg cagccacttc tcctgccgtc attccctgac gtactgatgt
    32761 taacagcgcc aacaactcgt cagcagattc cgcgacgata actggcgcgt cgggttccct
    32821 tacgggttgc ttcggctggc ggttaaagcc agtaaccagc cactgggtgt catcgtctgg
    32881 ccgaccgatc tccttgcggc tgatgacacg cccggtacgc atcgcggcag cacaacctgc
    32941 gtccagcgcc gcacgacacg ccgccagatc gccttccacc accagcgtta agcgaccagg
    33001 gtcaagcact tcgtgactga gcagacgaac gttagctgct ttgagcatgg catccgctgc
    33061 atctatcgcg gcgaccatgc cgtccacttc cagcaatccc agtgcattga tcatcggcaa
    33121 cctccgttac gcacgctgga ttggattacg cgcgatttcc agcactgcat cggtaaaggc
    33181 gttacaggct gctttacacg cggcctggct accggttaaa aatgctgccg agtagttggt
    33241 ttcagacggt ggcgggacat aggtcaccag ctgtacgtcg gcagatttca acgctgcatc
    33301 aatgccgtag gtcgcttcca gcggcggtgc caccagatac gccatcggat cgccaagcgt
    33361 gatcccggcg gttgatgaga gataagaacc ggtacgcgaa actacatgtg ccaggaaggc
    33421 ggtgttttct gcgtcattgg cccactggaa agctgcgcca ttttcaatat gtgcaaccat
    33481 cgcatccaga ccggcacgca cttccgccgg gtttggccca ccaagcataa tcagcacttc
    33541 accggcagtc ggtgacgggc cgtgtgccgc tcccgcatac agcgagcgac catataccac
    33601 ttcgaccatc gcttgtttgg tcgcttcgtc agccgcaatg taggtgacgt cgtcagaatc
    33661 ggcagaaatc agcccgaggc tacgaatatg cggcggcaat tttagttcac gcgcaaattc
    33721 agcgttaacg gaggcaatca cccgcatggc ggttaccgac ggtcgaatca aatctaaagc
    33781 tggcatgatg cctccttatc gggtcatgtt gatgccggat gctttctgct ccagcatacg
    33841 tttcgccaaa tccacaatga cggctgcggc ttcaaccggc ggcgtgccgc cctggtgaat
    33901 gttagagata caggtgcgat cggcctcaac ggtggtcgcc atacgcggcg agtaaacggc
    33961 gtagcaggag aggctttctg actgccctaa ccccggacgt tcccccacca gcaggatcac
    34021 cactttcgcg ccgaggatct caccgatctg atcttcaatc ttcacgcgac cataacgaac
    34081 aaagaatggc gtgccgactt tcagcccggc ctgtttcagg cccgccatca gcggcggcag
    34141 gatctcttcg tagttcacgg tgatggcatc agttgacagg ccatcagaaa taacgacctg
    34201 tacgtccgga ttagcaacac actgtgcttt cagcgcttca acagcttctg cacacaggcg
    34261 gcggcccata tccgggcgag tcaggtagag atttttgtcg ctaatttcgg agcgtacttc
    34321 cagcaagccc tgcgctttca cccactcttc cggcacttct ttcagtacgg tatctttcga
    34381 acgagagtga tcggccagga aacgcagcaa cgcctgcgta cgcggacgcg gaccggcacg
    34441 accggtacaa acgcgggcca cggtgctgcg acgcagttct gttaatacgt ctgcgcgatg
    34501 cgggttttca acaccaatcc acgcttttgc ttccgcagaa cctaaatcca gcgcgcagct
    34561 ttccgaggtc accggtgccg cacagttggt ggtggcgcac tttgcttctg acggcgcggg
    34621 ggccgtttgt cccattgacg ccatcacgct gcgtacaatt tcttcaatct gtttttgatc
    34681 catgatgtgt tatctccgcg tcatcagaag aacagtgacg gatcgcccgc ccgtttggtc
    34741 aggcgaccgt ttgccataat gcccatgctt tccagccagc gttcaaactc cggtgacgga
    34801 cgcaggttga gtaactgacg cacagtggcg gtatcgtgga atgcggtggt ctggtagttg
    34861 agcatgatgt catcacccag cggcatcccc atgatgtagt tgcagcctgc ggtggcgagc
    34921 aggatcatca ggttttcgtt gaggttctgg tcagcgtcag cgtggttggt gtaacagcag
    34981 tcacagccca tagagatgcc gctcagcttg cccataaagt gatcttctaa gccagcacgg
    35041 ataatctggc ggtcgttgta gagatactcc ggcccaataa agccgaccac ggtgttgacg
    35101 ataaacggat cgtaatgacg cgccagccca tagttacgtg cttccatcgt cacctggtca
    35161 gcgccgaagt tagcgccagc ggatagcgca gagccttgtc cggtttcgaa gtagaggcag
    35221 ttttccccgg cgatacggtt gaactctgca cccactgcgc gcgcttcgtc gagcatcgcc
    35281 agttccacgc caaactcttt cagccctttt tcgctgccac agatactctg gaaaatcagt
    35341 ccgccaggcg cgccgcgacg aatcgcttcg atctgggtgg tgacgtgcgc cagtacgcag
    35401 ccctgagttg ggatgttgaa tttgtcgatc acgccgtaaa tggtgtccaa cacgcggctt
    35461 aagttttcca cgtcgtcagt taccgggtta acgccgatca ccgcatcgcc caccccaaag
    35521 gaaagccctt catagatttg cgcagcgata ctttgcacgt cgtcacgggt atcgttcggc
    35581 tgcaaacggg cgctgaaggt gcccggaata ccaatggtgg tattggcctt tttgattacc
    35641 ggcattttct tcgcgccgta gatcaggtcc gcgttggagc aaatcttcgc taccgccgcg
    35701 accacttccg aggtcagccc tttgcgggta aaggcaatgt cgtccacgct ggtttcatcg
    35761 ctcagcacat actcacgcag ttcgctgatg ctccagtttt taatctggtt gtaggccgtt
    35821 tcgttaacat cgtcctgaat cagccgcgtc acgcagtcat cttcataggc aatcaccgga
    35881 ttattgcgga tgtccgctac ggtcatttcc gacaacacct gctttgccgc cacgcgctcc
    35941 tgtgagcttg ccgctgcaac gcccgccagc acatcccccg aacgcagttc gttggcttta
    36001 gccagcacct cttttacatc cttaaactga tatacattgc cgaacaatgt ggtctttagt
    36061 ttcataagtc gttccctcag gaaggaaatg cgagtgattt caccgtcacc ggcacaaccg
    36121 atccgccaaa aagaggcgta ccaatgtcga tatagtcccc cgcgcggaca atcacttcgt
    36181 caatgactgc caacgggagt tgttgtagct gtgggcgcaa caacatgccc agagctttac
    36241 caaagtcctg cccggccacc accagcaggg gatgcggatt cggaaaacgc gcgacgaaat
    36301 cgaccagcgc gttgatgacc gtcagtaccg cggcgtaacg cacaggcagc gaggcgggaa
    36361 gcgccagcac gtacgcgtca gttttgggat caagatccag ctgaagcagc gcctgttgcc
    36421 aggcactcac cagatccgtt tcatcaatcg ggatcgccac cggcaaattg cgcagcggca
    36481 gttgtacgcc ctccagccag attgtgctgc cagagagcga aagggtatgt gcacccgcgc
    36541 caatcaccgt ggcgcgtacg gtttgcgccg gaaactgcac attcatctca cgcaggcgcg
    36601 gatggtcatg cagcgccgtt gccagaagtg ggccaatatc ggcaaaacag aacgggtcgg
    36661 cgggctggtg gcgataacat tcgcccacgc ctccagaaag cgtaatgatt tcgggcgtaa
    36721 cacctgcggg cagcaaaccg gtttgcatca atgcctgcgc gagcggcgaa agcgttccgt
    36781 caatcacttc gacaatcagc gccgccatcc gccgggtaac ctgcaccagc tgcgcgccgg
    36841 tcagcgaacg ggcgtcagtg cctgcaccga agcactcatc cacaatcatc tgccccggtt
    36901 tatgagcgta aaccacgcgc ccctggctgt cggtttccat caggcgacca ccgacgttga
    36961 gacaggcagt gccgctgatt tttccggcat cgaacagggc gtagttcgcg gtgccaccgc
    37021 cgatgtcgat attcagtacc cgacacagcc gttgttcaga aagggtttgc gccccagctc
    37081 cgtgaccggc gatcacggat tcgaggtgcg gcccggcgct ggcaacgaca aaatccccca
    37141 gcgattgaga gagcgccatc accgccgggc gagcattgcg ggttttcgcg ctttcaccgg
    37201 tgatgatgat ggcaccagaa tcaacgcttt ccggcgcaat acccgcagcc tgatattgct
    37261 cgagtattaa ggttttcagt tccgcttctt ttaaaccgcc ctgtttatcg acaggggtaa
    37321 agaacaccgg actttgccag ctaatttcgc gtttaatgaa ttcgtagcgc ggcacctgcg
    37381 acaccgccgc acggttaacc agctccaggt gggagaaaat cacctgggtg gtggtggtgc
    37441 cgatatcgat accgacgctc aatagctggc gagtgttcac gattgtgcct ccgcttcggt
    37501 tttagtcgcg gtcgcgtctt cttttggcac cagcatcatc gccacgccaa tcgccgttac
    37561 gccgccgatc aacttgccga caatcatcgg gaagatcatg gcgttcatgt tggcagcggc
    37621 gaagcctaag tggtcgccca gggcgaaagc agcggaaacg gcgaaggcgc agttgatgac
    37681 tttgccgcgg gtatccatct gcttcatcat gccgaacatc gggatgttgt tggcaagcgt
    37741 tgccaccatg ccggctgccg cgatgttgtt catattcagc actttaccga cgctcatcag
    37801 cggtttttca aaccagcgag tcagcagcag caccattgga tacgccccta acagcacgca
    37861 ggagatagaa ccgataactt caatggcgcg catcacttca ccgggtttat cgccaggggc
    37921 cataaagata ggatccagtc cggggatcag ttcccagcca aggaggaatt tcactaccgc
    37981 agcggcaaga ccgagggtga tcaatgccac gaggaatttg gcgaagatct ggaagccgtt
    38041 gatcattttt tccgggatga atttcagccc cagcgccacc agaatcgcga caatgatcac
    38101 cgggatcatg ttcatcagga tcagggcgaa agtgaattcc accggctgac cgttgatctg
    38161 cacaccggag tacatagcaa ccagaccacc agcaatacag ccaatcggaa tggtcacaat
    38221 gcccgccagc acgccgagcg ccagataacg acggtcagaa ggttcgataa tgccgagcgc
    38281 caccggaatg gaaaacacaa tcgttggccc catcatcgac ccgagaatta acccagagta
    38341 tagccacgct gctacgtcgc cgcccgccag ctctttggcg aggaagaagc cgcccatatc
    38401 gcacgccagc agtgttccgg cgaacatcga tgggttagcg ccgagcattt cgtaaaccgg
    38461 aataattacc ggcccgagta cgtgagccag taccggtgcc agcgcggtca taccgaccat
    38521 cgccaggccc agtgcgccca ttgccataaa gccttcttcg aactgaccgc ctgatccttc
    38581 gatactttta ccgaacttac cgaggaaacg agcagaaccg ccgaactgcg acaggatcct
    38641 gtctacggca gctatcagca taaagaacat catgatgtac atgatgattt cgttaattcc
    38701 catcgccttt actccctgtt agttgttatt tattggcgga tgcggcgtaa acgccttatc
    38761 cgccctacat gtgcaatccc gaaggccgga tcagacgcgg caagcgtcgc atccggcatt
    38821 tattgcgccg ctgcgtacag gccgacaatc tgctccaggc tggcggtacg cgggttgctg
    38881 cgcagacaaa tatcttcctg cgcggcctgc gcccatgcac cgtaatgcgc agatgtcgcg
    38941 ccaacatcgc ccagtcgttt accaatccca acttccgcaa tcagctcact taccgcgtta
    39001 atagcgtcac gatcgtcgga ttttttagtt cgcagtgccc gaccaatctg actaaaccgt
    39061 tcgcgacaaa ccatccggtt aaattccatc accgttggca gcaacatggc gttcgccaga
    39121 ccgtgtggaa tatgcagcgc cgcccctggc tgatgcgcca tcgcgtggca caacccaagc
    39181 cccgcattgg aaaacgccat tcccgccata catgaagcca gtaacatgct ctcgcgcgcg
    39241 gcaaggtcat gaccgtagcc caccgctttc ggcagcgatt tgccaatcat cgcaatggca
    39301 ccaatcgcca gactgtcggt aaacggtgta gcgttcaggg cgctgtatgc ttcaatggca
    39361 tgggttaacg catcaatgcc ggtcatcgcc gtgacatgcg acggcacacc ttcggtcaat
    39421 gcggcgtcga gaatcgccac atccggcatc agcgaggcat gggctaacac ctgcttacgc
    39481 ccgctcaccg catcgataat caccgttacg ttggtggttt cagagccggt tccggcggta
    39541 gtgggaatgg caatcaacgg caggcgcgat tgcagaacgc tggtttctga catctctgcc
    39601 agcgtgctat ccgggttcgt caccagcaac gccacggctt tcgccgcatc cagcaccgag
    39661 ccgccgccaa atgcgatcac cccatcacag cctgactcac gcaactgcgc cacggctgca
    39721 cacacgtcgg tgatgcacgg ttcgcccacc ggacatggcc agagcgtcat ggcgatacct
    39781 ttaacggcca ggctacgcgt cagcccggcg gtcatccctg cctgatgcaa aaagctgtct
    39841 gccatcacga acagatgttt cagcccacgc gtttgcgctt gctgtccgca actgctcacc
    39901 gagcccggac cgcaaagcgt caccggtgga acgctaaatg tttttacccg ttgcagattc
    39961 agggtatcga acgcctgaaa gagcgcggtc tgcaattcat tttgcatata gcccctccgc
    40021 tttctctcgc ccactactgg cgatcgccag cggggtcata aacaaactgt gctgcggtaa
    40081 atgcacctgt aacgccggga attgtttgcg aaacagttcc gccacgcccg gttgcataca
    40141 agagccgccc gccagccata aatcagtaat gccctgccct tcaatatgcc gggcgacaat
    40201 gtccgccatt ttttcgtaca ccggtttcac cgcgggccaa atctcgtcac catgaccgcg
    40261 cttgtactgc tctgcctctt ccagcgaaat acgacgattt ccggcgagag tcagagagat
    40321 gtgatgtccg ccggtggctt catccgccga gtacgtcacc ttgccctttt tcacgatggc
    40381 aatgccggta gtgccgccgc cgatatccac tacaccggcg ttgtccagtt gcagcagatc
    40441 cgcgactgcg gttggctcat ccagcacatg gctcacttcc agcccggcag attccagcac
    40501 gttgatggaa atacgcgggt ccgtacctgg aggaaatgaa gtcgccgcat ggctaaaacg
    40561 aagaccgaat tgctgctcaa gagtgtcgag atggcgacga acaatggtga ccgcaccgaa
    40621 gaaatcccag acgatgccgt cgcggaccac atcggcccag tcgaggcata ccgccaccgg
    40681 ctgaccgtcg cggtcaacaa ccatcgacac cacatcgcag gtgcccagat ccacccccag
    40741 ccacagcgga gattccgtcg cggcaggcgt ctggttacac agcgttgccg ccgtttgcag
    40801 acgtggggtg agccattgtt cgtcgtgcgc catcggttac tccttaaaca atgcgaaacg
    40861 catcgactaa tacacagcga cgcagacgga caaacgtccg cgcgctggtt accccttcac
    40921 cggttggcgt ggtgatggtc atggtggtcc agccttcccc gcccagcccc agcccggcaa
    40981 tgcacggtcc gttcttaacg aaaatgctgg tatcaatggc gttcgccatc tggttcatgt
    41041 tttcgatatt gcgcgagtgc attgccgccg tgtggtggca accgccctcc agtttcaccg
    41101 ccagcgcaat ggcatccgcc acgttggcga cgcgcacgac gggcagcacc ggcatcatca
    41161 gttcggtcac ggcaaacgga tgttctgcag tggtttccac aaacagcaaa cgcgtttctt
    41221 gcggcacgtt caggccgatt gccgccgcga ttttcgccgc atcgcgacca acccagtcac
    41281 ggctgacggt gccttttccg cgctcgtcga tatttttcag caacaccggt tgcagctgct
    41341 gcgcctgttc tgcggtcagt ttcaccgcgt gctggccttc catcagacgc atcagttcat
    41401 cggctacgct atcaacaaca atcagtacct tttcgtcggc acaaatgatg ttgttatcga
    41461 aagaagcgcc tttgacgatg gactgagcgg cacgggcgag gtcggcggtt tcatccacca
    41521 ctaccggcgg gttgccagcg cctgcggcaa tcagacgttt attggtgtgt ttacgcgccg
    41581 cttctactac cgcttcgccg ccggttacca ccagcaggcc gatacccgga aacttgaaca
    41641 agcgttgcgc ggtttcgata tccggatttg ccacagtaac cagtaagttt tccggcccac
    41701 ctgcggcaac aatcgcctgg ttgagcagcg taatcgcccg ctgggagact tttttcgccg
    41761 ccggatgcgg ggcaaaaatg acgctgttgc ccgcggcaat caggctgatg gcgttgttaa
    41821 ttacggttgc cgccgggtta gtggaaggcg tcaccgaagc caccacgccc cagggtgcgt
    41881 tttcaattag ggtcaggccg ttgtcgccag tcagcacttg cggagagagg cactcaacgc
    41941 ctggtgtgcc gcgcgcctga gcgacgtttt ttgcaaattt atcttcaacg cgccccatgc
    42001 cggtttcact gacggcaagt tccgctaaat ctctggcgtg tttttcgcct gcttcacgaa
    42061 tggcagcaat ggctaactgg cgcattgcca cgctttttaa cccttgctgg gcgactttgg
    42121 ctgccgcaac ggcgtcatcc agggacgcga aaacgcccat ctcatgaacg gcggcggacg
    42181 gcgtgtcact gctttgcatt ttcagcagta ccgctttcac cacctgttca atatcctgtt
    42241 gattcatgat gttctgcctt atttgtggaa aattacctga ccgccagaca ccacctcatc
    42301 gacaatgcca atcacgcaca gatcgaccgg tgacgtttcg cttttatgcg cctggcgggc
    42361 ggaactgcca ctcaccagta acacccactc cccggttccc gcgccaatat tgtcgatggc
    42421 gacggcgcat tgcccgtcag gattaccttg tggatcaatc atttccacca tcagcaattt
    42481 gtcatgcgcc agtccgtgat ggcgtacggt acaaacaatt tgtccagtga cgactgccag
    42541 tttcataccc gcctccgtgg cgtatttcag gtaaaagctc cccctaccct ccgcagaagg
    42601 taaaatgaaa aaggagagag cgtgacgccc gaatcgacgt cacacagggt gattacaggt
    42661 tgctgctatc gcctttcagg ccgatcggga agacttcttc caggtcgccg tgcggacgcg
    42721 ggataacgtg tacagatacc agctcgccga tacgctgtgc cgctgcggca cctgcatcgg
    42781 tcgcggcttt acaggctgca acatcaccac gaaccatggc agtacacagg ccgccgccaa
    42841 tctgcttaac accaaccagc ttgacgcgcg cagctttcac catcgcgtca gaagcctcaa
    42901 tcagtgcaac caggccccgg gtttcgatca ttcctaatgc ttccattgtg ctttcctctt
    42961 tatcagggtc cagaacggga ccgttcattc aaccagtgtt tgtaaactgc tttcgcggtt
    43021 cacttctgtc tgacgcggca cagctgccac cagcgccagc tcgataattt cctgcacgct
    43081 acaaccacga gagagatcgt gcatcggcgc ggcaagtcct tgtatcagtg gcccgacggc
    43141 acgatatccg ccgagtcgtt gtgcgatttt gtaaccaata tttccggctt ccagcgacgg
    43201 aaaaaccatc acattggcct tgccctgtag cgggctggca ggcgcttttt gcgccgccac
    43261 ttccggcacg aaggcggcgt caaactgtaa ctcgccatcc accaccagct ttggtgcgcg
    43321 ctcacggacg atttctgtcg cctgctggac gttagcaaca caggggtgac gggcgctacc
    43381 attgctggaa aacgacagca tcgccacgcg cggctcttct ccggtgatgg cgcgccaggt
    43441 ttcggcactg gcaagcgcga tatccgccag ctgcgccgcc gtcggctgtg gcaccacgct
    43501 gcaatcggca aagcccaacg ccgggccgct gtactgtggc aacatcagga aaatggaaga
    43561 gagcgtttta cagcctggct gcaagccgat aatgcgtaat ccggcacgca gcacatttgc
    43621 cgtggaagag aggttgcccg cgatacagac atccgcttta ccggcgctga ccattgctgc
    43681 ggcgaacatc agagggtcgg taagtttttc cagcgcatcc ggcggcgttt tttcgcccgc
    43741 gcgggccagc cagcgatgag caaattcttc ccgcattgcg aggttgccat gcggatcaat
    43801 cacctgtagc ccgtccatcg ccacgccgtg actgagcgca aactgacgaa gttcaaacgg
    43861 attggcgacc agaatgggcg ttgccagacc ttgttgatgt aaatattgcg cggctttcag
    43921 cacacgttgg tctaacgcat ccggaaaaac cactctggcg ggcgctcgca acgccagttc
    43981 acgacaacgt tcaataatca ttgcgtttcc cccagtcgtt gttggatctg cttcaccgtt
    44041 agcggctgct tcgttacgct caggatcatc atcacgtaga ccgtgctgga aaggcggttc
    44101 agcgcctgca aaatgtccgg gcgtaatact tcaaagctgc gggtgataaa cacctgcgcc
    44161 gctacggttt cggtttcccg cactttggta cgtagcagat tgagcaacgc cgcatcgcgc
    44221 ccgtggctgg cttccggcac cagatgatcg tggtcgagat agcgcagcgg ctggtgagaa
    44281 agtcgatgca gatcttcgtc agaaagcccg acaatcgcct ggcagcccag cggttcaccc
    44341 agcgcatcgg cgcgcataat gttgccaaga cgcgaacgga tatccgccag ccacggctgc
    44401 cacggttccg ccagttcaat ttgcagccac actgccagcg caatggtgct gtcgagaacc
    44461 gcgcgaaaac ccagacgcgg atcgcttttg gcgaccattt tttccgccga caggtgagtc
    44521 agcgtatccg gcttttttgc caccggctgg cggcacagtt cgcagcacgc ctgcggatgt
    44581 tcatcgctac tggtcagccc atgaacgggc tgcggctgct gctgttcatc gtcaacaaac
    44641 aggcggccct gctcgtcaat aaacttgatg cgcagatggc ggctttccag taactcccgg
    44701 gcagagggcg tcaggcgact gtccgcaggg agatggatct ctgcgccttc gctgagcgta
    44761 tggttcgctc ttagccatgc ttcggtgatg aaatctttca tagggattgc cagttagccg
    44821 gccaggcgac atacaggaat ttcacgctgg atgtcgtacc aaattcgata ctggagcctt
    44881 tcgggataaa catcacgtca cccgctttgg cgatcatggt ttcgccttcg tggcgaacat
    44941 gcagctcccc ttccagcacc atgtcgattt cgtcgtagtt cagggtccac gggaagaatg
    45001 cgttctccca ctgcataaaa ccagcggcca tgctgctgcc gtcgtctccg gtcacaagat
    45061 cagtcaagcc cacgcaatgc ggctctgcgc cgtcgaaacg accaaacttg acgctgctgc
    45121 catcaatcac tttgatgccg cctttgccgg tcaccgattt gaagctcggc tgcattgcgc
    45181 cctgctccag cgactgtttt tccttcatca ctttttccat cagctgtgcg accaggcttt
    45241 cggtaaactg gccttccggc agctgggcaa tgatggtttc gcggatgcgc tggctttcgg
    45301 ttttgtcggc tggcacgctg gcgggcacgg acgccgttac cgggatcgat tcatcacatt
    45361 cggtgatggt aaaacccagt agatccgcca cttcacgggc ttccggggta ataatgctgg
    45421 cgcgcagaac cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga atatcattcg
    45481 ctgtgatgag ttttttcacc tgcttcctcc tgtttggtga gagatgccag ataatccacc
    45541 agttgctgca cgctttgcgg gtcgtggctg tttagctcga aaatcggctc ttcgaaaccc
    45601 gtctcaagca ataactttcg cgttgcggcg acgtcagcat ctggcatgtc cgttttactg
    45661 atgacggcga tttgccgttt gctgacgcca atatccagca acccggcagg caaccgactt
    45721 tccgggtcat ttgcgccatg gacatagatc agcatgtcta catcctgtag cgtggtaatt
    45781 aaggcgtgat accagcgggg atggctaaaa tattcacccg gcgtatcaat atcgccctta
    45841 tcattaaatt ccacggcctg tgtttttctg gcgagggtat aattcccctg caacgcatta
    45901 aataacgttg tttttcctgc cccgacggta cccacaaacg caattcgttt catgaatgcc
    45961 tcgtattaac ttttggtcat ttcacaaagc gtataattta ataagcgacc taaaccgctg
    46021 acggtttgtg ataacgcttc ttctactgcg cccaccgagc cataaatcac caatgcaccg
    46081 ctgaagcggt cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa atcaccggcg
    46141 atcatcgccg tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc atccggaacg
    46201 ccgattttct tcgccagttc ctcaccggga tgcgcgatga gatgcgccag tgtgacctgt
    46261 ttgccgggca caaattcctg aatgatgcgt tctttatcca tgagtcgccg cctgtgaaaa
    46321 actttcgtgt atcgtgacaa tttttcgtca gagtaaataa caaaattcgg caagcgcgtt
    46381 taaaaggtga ggtagatcac taagaatgga gagatattcg ctgtggtgca taaactttca
    46441 tatgcaacgc atgaatataa ataaaataat atcgagatat tatatagact gcttttttta
    46501 ttattcgcaa actcagtaat aagctttact aatatattgc agccaaaccg cttaccctga
    46561 atattcaggg taagcgtgag agttaaaaaa gaattacagc ggttgggttt gcgcttcaac
    46621 cacggccagc gccaccatgt tgacgatacg acgcaccgat gcgatcggcg ttaacacgtg
    46681 aaccggtttc gcaacgccca tcagcaccgg accgacagtc acaccttccg agctggaaac
    46741 gcgcagtaag ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca gaatattggc
    46801 agaacctttc aaagggctgt ccggcatacg gtcgttgcga atcgcttcca ccagcgctgc
    46861 atcgccgtgc atttcaccat caatcatcag atctggcgca cgatccctga ccagttccag
    46921 cgcctgacgc attttgctcg acgacgggca gtcagaagaa ccaaagttgg agtgcgacaa
    46981 cagagcaacg cgcggctcaa taccaaaacg acggacagtt tctgccgcca tcaaggtgat
    47041 ctccgccagc tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa taaaggtgtt
    47101 accactcggc agcagcagcg cgttcattgc acctgcggtg tgaacgccat cgcgataacc
    47161 aaagacattt ttcaccacgc taaaatgttc atgataatcg cccaccgtac cgcagatcat
    47221 tgcatcggct tccccacgct gaaccatgat cgcgccgatc actgtcgggt tactgatcag
    47281 cgcccgctgc gcctgttcct gagtgacacc gcgacgcttc atgatctgga agtattcggt
    47341 ccagtactct ttaaagcgcg gatcggattc gttattgacg atctcaaaat caacgcccgc
    47401 tttgatctgc aagcccagtt tctgaatgcg catttcgatc acgttcggac gaccgataag
    47461 gatcggtttc gccagtccca gcgttaccag ttcctgagtg gcatgcagaa cgcgcgcctc
    47521 ttccccttct ggcagaacaa cgcgcttcgg cgctttgcga gcctgggaga aaatcggctt
    47581 cataaacagg ttggttttgt aaacgaactc agtcagcttg tcgatgtaga cgtcgaaatc
    47641 agcaatcgga cgcgtcgcca cgcccgactc catcgcggct ttagcgaccg caggagcgat
    47701 cttaacgatc aagcgcggat caaacggttt tggaatgatg tattccggac caaagctcag
    47761 atcctgatcg ccatacgctg aagccaccac ttcgctctgt tcggcgtggg ccagttccgc
    47821 aatcgcgcgt accgccgcca gtttcatctc ttcattgata gccgttgcgc caacatccag
    47881 cgcgccacgg aagataaacg ggaagcaaag gacgttgttg acctggtttg gatagtcaga
    47941 acgaccggtg caaatgatgg catccggacg cacttctttc gccagcggcg gcagaatttc
    48001 cggttccggg ttcgccagcg ccaggatcat tggcgcacga gccattttct tcaccatttc
    48061 ctgggtcagc actttcgggc cggaacatcc aaggaaaata tccgcgcctt caatcacatc
    48121 atcgagggta cgtttgccgt catccaccac cgcatatgcg gctttggttt ccgccatgtt
    48181 tggctcacgc ccctgataga taacgccttt tgaatcgcaa accacgatgt tatgtttttg
    48241 cagacccagc gctaccagca ggttcataca ggcgattgct gcggcacccg cgccggaaac
    48301 caccatccgc acgtcggaga tgtttttctc caccacgcgc aagccgttga ggatggcggc
    48361 agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat accggaatat tcatccgctc
    48421 gcgcagtttc tgttcaatat agaaacattc tggcgcttta atgtcttcga ggttgattcc
    48481 gccgaaggtc ggttcgagcg cagcgacaac ttcaataaat ttgtccgggt cgagttcatc
    48541 aacttcaatg tcaaatacat caatcccggc gaatttctta aacagaacgc ccttgccttc
    48601 catcaccggt ttgccagcca gcgcgccaat gttgcctaac cccagcaccg ccgtaccgtt
    48661 agagatcacc gccaccaggt taccgcgcgc ggtatatttg taggctttta acgggtcttt
    48721 ttcgatttca agacaaggtg cggcaacgcc tggtgagtag gccagcgcca gatcgcgctg
    48781 cgttgccaga ggcttggttg gagaaacctg gattttccct ggaactggaa attcatggaa
    48841 atcaagtgca ctttgtttta actggtcatc catttggttg ttcctttcac gtaacgttca
    48901 caaataaagt gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta ttaccctgcc
    48961 ggagtctaca gactttgagc aagtccaaac tctcaccatt aatataatgt tttggtaata
    49021 atcctataac actgatgtta cctgcttaat ccagcaatac catgcctgtc tgctatgctt
    49081 ttttgatgcg tttagcgaaa tttctcagaa gtgtgaatta acgcactcat ctaacacttt
    49141 acttttcaag gagtatttcc tatgaacgag ttagacggca tcaaacagtt caccactgtc
    49201 gtggcagaca gcggcgatat tgagtccatt cgccattatc atccccagga tgccaccacc
    49261 aatccttcgc tgttactcaa agctgccgga ttatcacaat atgagcattt aatagacgat
    49321 gctatcgcct ggggtaaaaa aaatggcaag acccaggaac aacaggtggt cgcagcgtgt
    49381 gacaaactgg cagtcaattt cggtgctgaa attctcaaaa tcgtacccgg tcgcgtgtca
    49441 acagaagttg atgcacgcct ctcttttgat aaagaaaaga gtattgaaaa ggcgcgccat
    49501 ctggtggact tgtatcagca acaaggcgtt gagaaatcac gcattttgat caaactggct
    49561 tcgacctggg aaggaattcg cgcggccgaa gagctggaaa aagaaggtat taactgcaac
    49621 ctgacgctgc tgttttcttt tgcccaggcg cgggcctgtg cagaagcggg cgtttttctg
    49681 atttcgccgt ttgtcgggcg tatttatgac tggtatcagg cacgcaagcc gatggacccg
    49741 tatgtggtgg aagaagatcc gggcgttaaa tcggtgcgca atatctacga ctactataag
    49801 caacaccact atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga acaaatcctc
    49861 gccttaaccg gctgcgatcg actgactatc gcaccgaatt tgctgaagga gctgcaggaa
    49921 aaagtttcgc cagtggtacg taaattaatc ccgccttctc agacgttccc acgcccagca
    49981 cccatgagcg aagcggagtt ccgttgggag cacaatcagg atgcgatggc ggtagaaaaa
    50041 ctgtctgaag gcattcgtct gttcgctgtt gatcaacgca aactggaaga tcttcttgcc
    50101 gccaaactat aaaccagcca cggagtgtta tatgtcccga aaagaccttg ccaatgcgat
    50161 tcgcgcactc agtatggatg cggtacaaaa agccaattct ggtcatcccg gcgcgccgat
    50221 gggcatggct gatattgccg aagtgctgtg gaacgatttt cttaaacata atcctaccga
    50281 cccaacctgg tatgatcgcg accgctttat tctttccaac ggtcacgcgt cgatgctgct
    50341 ctacagtttg ctacatctga ccggttacga cctgccgctg gaagaactga agaacttccg
    50401 tcagttgcat tcgaaaaccc caggccaccc ggagattggc tatacgccag gcgttgaaac
    50461 caccaccggc ccgcttggac aaggtttggc gaacgccgtc gggctggcga tagcagagcg
    50521 tacactggcg gcgcagttta accagccaga ccatgagatc gtcgatcact tcacctatgt
    50581 gtttatgggc gacggctgcc tgatggaagg tatttcccac gaagtctgtt cgctggcagg
    50641 cacgctggga ctgggcaagc tgattggttt ttacgatcac aacggtattt ccatcgacgg
    50701 tgaaacagaa ggctggttta ccgacgatac ggcaaaacgt tttgaagcct atcactggca
    50761 tgtgatccat gaaatcgacg gtcacgatcc gcaggcggtg aaggaagcga tccttgaagc
    50821 gcaaagcatg aaagataagc cgtcgctgat tatctgccgt acggtgattg gctttggttc
    50881 gccgaataaa gcaggtaagg aagaggcgca cggcgcacca ctgggggaag aagaagtggc
    50941 gctggcacgg caaaaactgg gctggcacca tccgccattt gagatcccta aaaagattta
    51001 tcacgcctgg gatgcccgcg aaaaaggcga aaaagcgcag cagagctgga atgagaagtt
    51061 tgccgcctat aaaaaggctc atccgcaact ggcagaagag tttacccgtc ggatgagcgg
    51121 tggtttaccg aaggactggg agaaaacgac tcagaaatat atcaatgagt tgcaggcgaa
    51181 tccggcgaaa atcgccaccc gtaaggcttc gcaaaatacg cttaacgctt acggaccgat
    51241 gctaccggag ctgctcggag gttcagcgga tctggctccc agcaacctca ccatctggaa
    51301 aggctctgtg tcgctgaagg aagatccggc gggcaactac attcactacg gggtgcgtga
    51361 atttggcatg accgctatcg ccaacggcat tgcgcaccac ggcggctttg tgccgtatac
    51421 cgcgacgttc ctgatgtttg ttgaatacgc ccgtaatgcc gcgcggatgg cggcactgat
    51481 gaaagcacgg cagattatgg tttataccca cgattctatt ggcttagggg aagatggtcc
    51541 gacgcaccag gccgttgagc aactggccag tctgcgctta acgccgaatt tcagcacctg
    51601 gcgaccgtgc gatcaggtgg aagcggcggt gggctggaag ctggcggttg agcgccacaa
    51661 cgggccgaca gcactgatcc tctcaaggca gaatctggcc caggtggaac gtacgccgga
    51721 tcaggttaaa gagattgctc gtggcggtta tgtgctgaaa gacagcggcg gtaagccaga
    51781 tattattctg attgccaccg gttcagagat ggaaattacc ctgcaagcgg cagagaaatt
    51841 agcaggagaa ggtcgcaatg tacgcgtagt ttccctgccc tcgaccgata ttttcgacgc
    51901 ccaggatgag gaatatcggg agtcggtgtt gccttctaac gttgcggctc gcgtggcggt
    51961 ggaagcaggt attgccgatt actggtacaa gtatgttggt ctgaaagggg taattgtcgg
    52021 gatgacgggt tacggggaat ctgctccggc ggataagctg ttcccgttct ttggctttac
    52081 cgccgagaat attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg cctgatggtg
    52141 atcgccggat gctgattgcc ggatgcgacg ctgacgcgtc ttatccggcc tacagcgtct
    52201 tatccggcct acatgtcccc gcaattttgt ttaacgggtg atccacaacg tgggccaggc
    52261 gtctggccca tgccagttat cgcaggtggg ttctgcggca taacgcacca ggcgaaaacg
    52321 ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg cttaatccgc gccctttcgc
    52381 taaggtcacc agttcacgcg atttctcatc aaatgttgcg ttcatcagtt ccagttcatt
    52441 cgtctcctga ccgttgttga atggcaaacg caaccgaacc gggcgcgagg ctagtggctt
    52501 tttacgcgac acaatccatg ccaaatcaat ggtgttatag gcccctgcct cacagctaat
    52561 catcatcagc gctttatcat cagtcagcgc agtgacattc acctcacgac gtaacggatc
    52621 aagcgagcag cgcagaccat tcatccgcca gtttccataa tccagcaaat cgttgcgttc
    52681 ttcgagtgag agtggcgtcg gcgttgggtt aaccaccgcg acctctttca gcgcaggcgc
    52741 gggcggtacg ctgagcggcg gttcgtcccc tttcttgatc cacgcggttt cactgccaac
    52801 gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt aagccactca gagaaatggt
    52861 ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga atcacctgca aaaacgcggt
    52921 gatggttgcc gtatcatcgg ttactaataa ccatggtgaa atccgccact tgtcaccact
    52981 taacgccaac ggctcaccat ctaacagcag ccgtggcgct atctctcctt cagacgcatc
    53041 cggcgacttc aacccgccgc gctcaatacg taaaacggca tcggtatgcg ccccggcgct
    53101 gcggctcagg gtcatcacca gtccattatg atcgcccgta ttgcgcgcca cgcagaagtt
    53161 ttggttatta caggtgacct gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac
    53221 caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa atgcgatagc gcatggacgg
    53281 tacgacccca gaagttcaaa caaacagcac aagtcgtatc ttcgttgcgc aggtagcgcg
    53341 gctcaatcag atttgtcgga ttgagccaat aataaacaac gaaatattat tgttattttt
    53401 cagtccatta aatgtgacgt ttgcaaatag ttaagcaata acaccgtctt accgtcacgt
    53461 atctcgccgg ttttgatcat ctccagcgcc tggctgaacg gcagctcgag cacttcaata
    53521 tcttcatctt caacaccgcc accggcgtta gcgcgctgat tgtcactgta ttcggcgata
    53581 aaaaagtgga ttagctcagt cacaccgcca ggcgacatat acagttcaaa taatttgcgc
    53641 acttcgccca cttcatagcc cgtctcttca atcgcttctt tacgaatgca cacttccggt
    53701 tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct gcccgctttc attgccatta
    53761 acccaggtag cgacacggaa ctgacgaatc agaaccacgc tctttttctt cgcgttgtac
    53821 aggaggatcg tcgcaccatt accgcgatcg taaacttcac gtttatggcg gataacttcg
    53881 ccatctttgc gcgtgagatc gtaggtaatg ttgtgcaggg tgaaatagtt atcggagaga
    53941 attttgtctt taatgagggt gatttgttgc gtcataccga ctccacagcg cgaaatgaac
    54001 aattatctta cgctgtgaag tcgggtttgt ctgcgcaggc tatcagtgag atttgactga
    54061 ttttaccccc agccagtcaa taatcccctg tgccgcatga cgtccttctg ccattgcggt
    54121 aaccaccaga tccgcaccac gcacggcgtc gccaccggcg aagattttcg gattggtggt
    54181 ctggtaacgg tactggcttt ccacatccgc gatgatgcgg ccccatttgt ctaccgttac
    54241 accgtgcgac tccagccacg gcatcccgtg tggattgaag ccaaacgcca taatcaccgc
    54301 atcggctggc atgacaaatt cactgccctc aatcggtaca ggtcgccgac gcccctgggc
    54361 atccggctcg ccaagacgcg tgcgcaggaa acgaatcccg cagacgtgac cttgttcatt
    54421 cagctcaagc gccaccggct ggacgttaaa ttcgaagttg gccccctctt cgcgggcgtt
    54481 cctcacctct ttcttcgagc ctggcatgtt agcttcatca cgacgataag cgcaggtgac
    54541 gttactcgcg ccgtggcgca gcgcagtacg cacacagtcc atcgcggtgt cgccgccgcc
    54601 cagtaccacg acgttaagtc cggcggtatt gataaacggc tcttccggta gctcttcgag
    54661 ccccatcacc tgtttagtgt tggcaatgag gaacggcagc gcgtcataaa cgcccggcgc
    54721 atcttcattg ggtaaacccg cgttcatgga acggtaagtg cctacgccaa cgaagaccgc
    54781 gtcgtattgt tccaaaagcg aatccaaaga gacatcttta cccacttcac aattgagttc
    54841 gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt gccagcaggg atttatccag
    54901 tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt tctggatggc gatcgtacac
    54961 cgtcaccgcc acgccattgc gggtcagaac atccgcacag gccagccctg ccggacctgc
    55021 cccgataatc gccacccgct tgtccacttt ggtgacatga cttaagtcag gacgccaacc
    55081 tttcgccaac gcctgatctg aaatatagcg ttcaatattg ccgatagtta ccgcgccgtg
    55141 ctcatcgcga atagtacagg caccttcaca caaacggtct tgcggacaaa cgcgtccggt
    55201 aatttccggc agggtgttgg tctggtgaga aagctcgacg gcggcgtcga tgtttccggc
    55261 tttcaccagt tcaatccact gcggaatatg gttatgcagc gggcaggtcc attcacaaac
    55321 gctgtgctcg ccgcacttaa ggcagcgcga ggcttcccgt tgtgcctggt cggcgcgaaa
    55381 tggcagataa atttcatcaa aaccggtttt gcgcgcttca atcgccagtt tatccggctc
    55441 gccacgcgcg ggcgttgcct gcatttgttc gactttactc attaccggca tttcttgcgc
    55501 cgcggtactg gcatgccacg gttgatgttc ctgacgcgcg gtgcgcaagc ggcggggttt
    55561 cgccatgccg gagagtgcga cgtcagtgac cagttgcagc gcgtccgccg ggcaattctc
    55621 aacacaggca ggaccgtttt cgcgccccgc acaaaggtcg catttatgcg ccgtggcttt
    55681 tacttttcct gccgcgacgg gtgtcaggac gatttgcatc gtaccaaaag gacaggccac
    55741 cacgcaggat ttacagccaa tacacttttg ctgattgacc tgaatgctgt catcaacgtg
    55801 gctgattgcg ccattagggc agctacgggc gcagggcgca tcttcacaat ggtgacaggt
    55861 cactgcacta cgttgctgtt gatgtttgat aaccgtaatt cggggatgaa aatggtgttg
    55921 gctcaggaca tgttgctcat cattgtgagc catgacacag gcgatttcac aagcatgaca
    55981 acccagacat tgctgactgt tggccataat aaaacgattc ataacgacct tcttttttgg
    56041 ttgtaaaaac cttattcttt atatgagtgt tgttattacc cgacttacag gggaatcggc
    56101 aatgttcatg tgcccagaat aagtaactat ttcgctataa actgtggcag atcaaataat
    56161 cccatcactg actaaattgt gtttcagcta actggaacat taatgatttt ttgtggagaa
    56221 gacgcgtgtg attgttaaac gacccgtctc ggccagtctg gcccgggcct ttttttacat
    56281 tgtgctgctg tcgattcttt ccacgggtat cgctctgcta actctggcga gcagtttgcg
    56341 cgacgctgag gctatcaata ttgccggatc gctacgtatg cagagttacc gcctgggcta
    56401 cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc cagttgtttc agcaggcact
    56461 gcattcaccg gtattaacca atctcaacgt ctggtatgtg ccagaagcag taaaaactcg
    56521 ctatgcgcat ctgaatgcca actggctgga gatgaataat cggctcagca agggcgattt
    56581 gccgtggtat caggccaata ttaataatta tgttaatcag atagacctgt tcgtgctggc
    56641 tttacagcac tacgctgaac gcaaaatgct gctggtggtg gcgatttccc tggctggcgg
    56701 tatcggtatt ttcacgctgg tcttttttac tttgcgccgt atacgccatc aggtggttgc
    56761 cccgctgaat cagctggtta ccgccagtca acgtattgaa cacgggcagt tcgactcgcc
    56821 gccgctggat accagtctgc cgaatgagct tggtctgctt gcaaaaacct ttaaccagat
    56881 gtcgagcgag ctgcataaat tgtacctttc gctggaggcg tcagtagaag aaaagacccg
    56941 cgatctccac gaggccaagc gtcgtctgga ggtgttgtat caatgttccc aggcgctgaa
    57001 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    57061 agcggctgaa tatctggagt taaatgtcgg tgaaaactgg cggattagcg aagggcaacc
    57121 aaacccggaa ttgccgatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    57181 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    57241 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    57301 gatggaagaa cgtgcaacca tcgcccgcga attgcacgac tcgctggctc aggtactttc
    57361 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    57421 acaaagtatc atggccgatt tttcccaggc gttgaatgat gcttatcggc agttacgcga
    57481 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    57541 aatgctggat acgttacaaa atcaaaccag cgccaaactg accctcgact gccgtctgcc
    57601 aaccctggcg ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    57661 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    57721 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    57781 acccgaaggt cattatggtc tgaatatcat gcgcgaacgc gcggaacggc tgggtgggac
    57841 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctctgc
    57901 ggagggtgag gaaagtcagt tgatgtaatg cctcctactg accaaagaat acttgcactt
    57961 aaggttcagt ataaaagggc atgataattt acattaactc ctttttttct ccacgattgg
    58021 ctcgtacctt gccgctacag tgaagcaagt caagcctaca acgatacgca gaaacacgag
    58081 gtcctctttt aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa
    58141 tcctgttgtg tctgacaggg accctggcga ttttttcatt gcccgttgaa caataccccg
    58201 atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc
    58261 tggaaaacac cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctcatgt
    58321 atatgtcatc tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag
    58381 gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa
    58441 agttaccgca ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaata
    58501 ttctgaccat tgctttcgtc tctaccgatg gttcgatgga taaacaggat attgccgatt
    58561 atgttgccag taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg
    58621 cctatggttc gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc
    58681 agatgacggc taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg
    58741 ggcaacttgg tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc
    58801 agtcactgct gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaagacg
    58861 gctcagaggt aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt
    58921 atcttagccg cttcaatggt aagccagcct ccgggctggg ggtaaaactg gcctccggcg
    58981 caaacgaaat ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtact
    59041 tcccgcacgg actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca
    59101 ttgaagacgt ggtgaaaacg ctgctggaag ctatcgccct ggttttcctc gttatgtatc
    59161 tgttcctgca aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga
    59221 tgggaacctt ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg
    59281 cgatggtact ggcgatcggt ctgctggtgg atgacgcaat cgtggtggtg gaaaacgtcg
    59341 aacgtattat gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc
    59401 agatccaggg ggcactggtc gggattgcga tggttctttc ggcggtattt gtaccaatgg
    59461 ccttcttcgg cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg
    59521 cgatggtgct gtcagtactg gtagcgatga tcctcactcc ggctctgtgt gccacactac
    59581 ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg cttttttgcc tggtttaacc
    59641 agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta
    59701 gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc
    59761 gtttgccgac gtcgttctta ccgctggaag accgtggcat gtttactacc tcggtacaat
    59821 tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt
    59881 acttcaccca tgaaaaagac aacatcatgt cggtgtttgc cactgttggt tctggccccg
    59941 ggggtaacgg gcaaaacgtg gcgcgaatgt ttatccgcct gaaagactgg agcgaacgcg
    60001 acagtaagac cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa
    60061 ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat tagcggtctt ggtagttctg
    60121 caggttttga tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag
    60181 cacgtaatca gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata
    60241 acggcctcga cgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc
    60301 tgggcgttgc tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg
    60361 tgaatgactt tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt
    60421 atcgcatgct gccagatgac attaatctct ggtatgtccg aaataaagat ggcggcatgg
    60481 tgcccttctc tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct
    60541 ataacggtta ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta
    60601 cggcgatgga tattatggaa tcgttagtga agcagttgcc aaacggcttt ggtctggagt
    60661 ggacggcgat gtcgtatcag gagcgacttt ccggcgcgca ggctccggcg ctgtacgcca
    60721 tttccttgct ggtggtattc ctgtgtctgg cggcgttgta tgaaagctgg tcggtgccgt
    60781 tctcggtaat gctggtcgtg ccgctggggg ttatcggcgc gctgctggca acctggatgc
    60841 gcgggctgga aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg
    60901 cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccacgacc
    60961 tgtttgaagc gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc
    61021 tggcatttat cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc
    61081 agcatgcggt gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt
    61141 acttcgtgcc gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg
    61201 aataagcaat aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga
    61261 acctgctcag cgggttcttg ctgtttatac tttgtctcgg gaattactta cgaagcataa
    61321 cttcgataaa gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa
    61381 ttatgggtat tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga
    61441 tgactacctc cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta
    61501 tgtgatacac agcaacattt cgagat
//
LOCUS       DAZFJF010000014        58591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFJF010000014 DAZFJF010000000
VERSION     DAZFJF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.014"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            203..874
                     /locus_tag="ACHKAU_001023"
     CDS             203..874
                     /locus_tag="ACHKAU_001023"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO5530135.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            884..2080
                     /gene="yaiP"
                     /locus_tag="ACHKAU_001024"
     CDS             884..2080
                     /gene="yaiP"
                     /locus_tag="ACHKAU_001024"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO5530136.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2058..2639
                     /locus_tag="ACHKAU_001025"
     CDS             2058..2639
                     /locus_tag="ACHKAU_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5530137.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2641..3414
                     /gene="yaiO"
                     /locus_tag="ACHKAU_001026"
     CDS             2641..3414
                     /gene="yaiO"
                     /locus_tag="ACHKAU_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO5530138.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3599..3874
                     /gene="frmR"
                     /locus_tag="ACHKAU_001027"
     CDS             3599..3874
                     /gene="frmR"
                     /locus_tag="ACHKAU_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO5530139.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3909..5018
                     /gene="frmA"
                     /locus_tag="ACHKAU_001028"
     CDS             3909..5018
                     /gene="frmA"
                     /locus_tag="ACHKAU_001028"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO5530140.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5111..5944
                     /gene="fghA"
                     /locus_tag="ACHKAU_001029"
     CDS             5111..5944
                     /gene="fghA"
                     /locus_tag="ACHKAU_001029"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5530141.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6069..6608)
                     /gene="yaiL"
                     /locus_tag="ACHKAU_001030"
     CDS             complement(6069..6608)
                     /gene="yaiL"
                     /locus_tag="ACHKAU_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO5530142.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6710..7921)
                     /gene="mhpT"
                     /locus_tag="ACHKAU_001031"
     CDS             complement(6710..7921)
                     /gene="mhpT"
                     /locus_tag="ACHKAU_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO5530143.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8196..9209)
                     /gene="mhpE"
                     /locus_tag="ACHKAU_001032"
     CDS             complement(8196..9209)
                     /gene="mhpE"
                     /locus_tag="ACHKAU_001032"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO5530144.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9206..10156)
                     /gene="mhpF"
                     /locus_tag="ACHKAU_001033"
     CDS             complement(9206..10156)
                     /gene="mhpF"
                     /locus_tag="ACHKAU_001033"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO5530145.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10153..10962)
                     /gene="mhpD"
                     /locus_tag="ACHKAU_001034"
     CDS             complement(10153..10962)
                     /gene="mhpD"
                     /locus_tag="ACHKAU_001034"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO5530146.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10972..11838)
                     /gene="mhpC"
                     /locus_tag="ACHKAU_001035"
     CDS             complement(10972..11838)
                     /gene="mhpC"
                     /locus_tag="ACHKAU_001035"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO5530147.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11856..12800)
                     /gene="mhpB"
                     /locus_tag="ACHKAU_001036"
     CDS             complement(11856..12800)
                     /gene="mhpB"
                     /locus_tag="ACHKAU_001036"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO5530148.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12802..14466)
                     /gene="mhpA"
                     /locus_tag="ACHKAU_001037"
     CDS             complement(12802..14466)
                     /gene="mhpA"
                     /locus_tag="ACHKAU_001037"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO5530149.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14657..15490
                     /gene="mhpR"
                     /locus_tag="ACHKAU_001038"
     CDS             14657..15490
                     /gene="mhpR"
                     /locus_tag="ACHKAU_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO5530150.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15567..16649
                     /gene="lacI"
                     /locus_tag="ACHKAU_001039"
     CDS             15567..16649
                     /gene="lacI"
                     /locus_tag="ACHKAU_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO5530151.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16772..19846
                     /gene="lacZ"
                     /locus_tag="ACHKAU_001040"
     CDS             16772..19846
                     /gene="lacZ"
                     /locus_tag="ACHKAU_001040"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO5530152.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19898..21151
                     /gene="lacY"
                     /locus_tag="ACHKAU_001041"
     CDS             19898..21151
                     /gene="lacY"
                     /locus_tag="ACHKAU_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO5530153.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21230..22411)
                     /gene="cynX"
                     /locus_tag="ACHKAU_001042"
     CDS             complement(21230..22411)
                     /gene="cynX"
                     /locus_tag="ACHKAU_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO5530154.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22417..22887)
                     /gene="cynS"
                     /locus_tag="ACHKAU_001043"
     CDS             complement(22417..22887)
                     /gene="cynS"
                     /locus_tag="ACHKAU_001043"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO5530155.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22918..23577)
                     /gene="cynT"
                     /locus_tag="ACHKAU_001044"
     CDS             complement(22918..23577)
                     /gene="cynT"
                     /locus_tag="ACHKAU_001044"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO5530156.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23687..24586
                     /gene="cynR"
                     /locus_tag="ACHKAU_001045"
     CDS             23687..24586
                     /gene="cynR"
                     /locus_tag="ACHKAU_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO5530157.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24626..25909)
                     /gene="codA"
                     /locus_tag="ACHKAU_001046"
     CDS             complement(24626..25909)
                     /gene="codA"
                     /locus_tag="ACHKAU_001046"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO5530158.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25899..27158)
                     /gene="codB"
                     /locus_tag="ACHKAU_001047"
     CDS             complement(25899..27158)
                     /gene="codB"
                     /locus_tag="ACHKAU_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO5530159.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27497..29383)
                     /gene="prpE"
                     /locus_tag="ACHKAU_001048"
     CDS             complement(27497..29383)
                     /gene="prpE"
                     /locus_tag="ACHKAU_001048"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO5530160.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29423..30874)
                     /gene="prpD"
                     /locus_tag="ACHKAU_001049"
     CDS             complement(29423..30874)
                     /gene="prpD"
                     /locus_tag="ACHKAU_001049"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO5530161.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(30908..32077)
                     /gene="prpC"
                     /locus_tag="ACHKAU_001050"
     CDS             complement(30908..32077)
                     /gene="prpC"
                     /locus_tag="ACHKAU_001050"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO5530162.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32122..33012)
                     /gene="prpB"
                     /locus_tag="ACHKAU_001051"
     CDS             complement(32122..33012)
                     /gene="prpB"
                     /locus_tag="ACHKAU_001051"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO5530163.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            33251..34837
                     /gene="prpR"
                     /locus_tag="ACHKAU_001052"
     CDS             33251..34837
                     /gene="prpR"
                     /locus_tag="ACHKAU_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO5530164.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(34935..35210)
                     /gene="yahO"
                     /locus_tag="ACHKAU_001053"
     CDS             complement(34935..35210)
                     /gene="yahO"
                     /locus_tag="ACHKAU_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO5530165.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35357..36028
                     /gene="yahN"
                     /locus_tag="ACHKAU_001054"
     CDS             35357..36028
                     /gene="yahN"
                     /locus_tag="ACHKAU_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO5530166.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36045..36290)
                     /gene="yahM"
                     /locus_tag="ACHKAU_001055"
     CDS             complement(36045..36290)
                     /gene="yahM"
                     /locus_tag="ACHKAU_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO5530167.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(36702..37517)
                     /gene="yahL"
                     /locus_tag="ACHKAU_001056"
     CDS             complement(36702..37517)
                     /gene="yahL"
                     /locus_tag="ACHKAU_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO5530168.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(37760..38809)
                     /gene="yahK"
                     /locus_tag="ACHKAU_001057"
     CDS             complement(37760..38809)
                     /gene="yahK"
                     /locus_tag="ACHKAU_001057"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO5530169.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(38896..39852)
                     /locus_tag="ACHKAU_001058"
     CDS             complement(38896..39852)
                     /locus_tag="ACHKAU_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5530170.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(39849..40820)
                     /locus_tag="ACHKAU_001059"
     CDS             complement(39849..40820)
                     /locus_tag="ACHKAU_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5530171.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40813..42297)
                     /locus_tag="ACHKAU_001060"
     CDS             complement(40813..42297)
                     /locus_tag="ACHKAU_001060"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5530172.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42346..43329)
                     /locus_tag="ACHKAU_001061"
     CDS             complement(42346..43329)
                     /locus_tag="ACHKAU_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5530173.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43583..43993)
                     /locus_tag="ACHKAU_001062"
     CDS             complement(43583..43993)
                     /locus_tag="ACHKAU_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO5530174.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44292..45674)
                     /gene="yahJ"
                     /locus_tag="ACHKAU_001063"
     CDS             complement(44292..45674)
                     /gene="yahJ"
                     /locus_tag="ACHKAU_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO5530175.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(45684..46634)
                     /gene="yahI"
                     /locus_tag="ACHKAU_001064"
     CDS             complement(45684..46634)
                     /gene="yahI"
                     /locus_tag="ACHKAU_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO5530176.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46777..48195)
                     /gene="yahG"
                     /locus_tag="ACHKAU_001065"
     CDS             complement(46777..48195)
                     /gene="yahG"
                     /locus_tag="ACHKAU_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5530177.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48195..49742)
                     /gene="fdrA"
                     /locus_tag="ACHKAU_001066"
     CDS             complement(48195..49742)
                     /gene="fdrA"
                     /locus_tag="ACHKAU_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5530178.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49732..50595)
                     /gene="yahE"
                     /locus_tag="ACHKAU_001067"
     CDS             complement(49732..50595)
                     /gene="yahE"
                     /locus_tag="ACHKAU_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5530179.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50635..51240)
                     /gene="yahD"
                     /locus_tag="ACHKAU_001068"
     CDS             complement(50635..51240)
                     /gene="yahD"
                     /locus_tag="ACHKAU_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO5530180.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            51498..51995
                     /gene="yahC"
                     /locus_tag="ACHKAU_001069"
     CDS             51498..51995
                     /gene="yahC"
                     /locus_tag="ACHKAU_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5530181.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52087..53019
                     /gene="yahB"
                     /locus_tag="ACHKAU_001070"
     CDS             52087..53019
                     /gene="yahB"
                     /locus_tag="ACHKAU_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5530182.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(53061..54149)
                     /gene="pdeL"
                     /locus_tag="ACHKAU_001071"
     CDS             complement(53061..54149)
                     /gene="pdeL"
                     /locus_tag="ACHKAU_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5530183.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54291..58274)
                     /gene="ehaA"
                     /locus_tag="ACHKAU_001072"
     CDS             complement(54291..58274)
                     /gene="ehaA"
                     /locus_tag="ACHKAU_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO5530184.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT        13946 a        15904 c        14590 g        14151 t
ORIGIN      
        1 tatacataca aaacatacaa acacttaaca tatgaattgc ataaaaacat tacccagtcg
       61 aacctccggg tctgaaagcc cttgcttttc agttattagc aacatttaac atttataaat
      121 taacatctgg ctgcgccaga gattacgtct ctgagtgcat cgttgtgctt gtagtataat
      181 aaataaaatg gagtgtcatt aagtggataa ggttttagat tcagccctcc cttcttcggc
      241 aaataaaaga aagggtatcc ttgctattgg cgcacatcct gacgatatag aattaggctg
      301 tggcgcatcg cttgctcgtc ttgcgcaaaa aggaatttat atcgcagccg tggtgatgac
      361 taccggcaac tctggtacag atggaataat cgatcgccat gaagaatcgc gcaacgccct
      421 aaagatatta gggtgccacc aaactattca tcttaatttt gctgacaccc gcgctcattt
      481 acagctcaat gatatgattt ccgctctgga agacatcatt aaaaatcaaa ttccttctga
      541 tgttgaaatc atgcgggtat ataccatgca tgatgccgac cgccatcagg atcatctcgc
      601 tgtttatcaa gcttcaatgg ttgcctgccg cactattcca caaattctcg gctacgaaac
      661 cccaagtacc tggctttcat ttatgcctca ggtttttgaa tccgttaaag aagaatattt
      721 cacagtcaaa cttgctgcat taaaaaaaca taaaagccag gaacgacgtg attatatgcg
      781 tcatgatcgc ctgcgtgcag ttgcacaatt tcgcgggcaa caggtcaata gcgatctggg
      841 tgaaggcttt gtaattcata aaatgattct ttgagcaaat tgcatgaaaa cctggatatt
      901 tatctgtatg tctatagcaa tgttgctatg gtttttaagt acgctaagac gtaaacccag
      961 tcaaaagaaa ggctgtattg acgccattat acctgcgtat aacgaaggcc cgtgtctggc
     1021 gcagtcactg gataatctac tgcgtaaccc ttatttttgc cgggtaattt gcgttaacga
     1081 cggctccacg gacaataccg aagcggtcat ggcggaagtc aaacgcaaat ggggcgaccg
     1141 ctttgttgcc gtcacgcaaa aaaataccgg taaaggtggt gcgctgatga atggcctcaa
     1201 ttatgccacc tgcgaccagg tttttttaag tgatgccgac acctatgttc cgcccgatca
     1261 agacggaatg ggctatatgc tggcagaaat tgagcgcggt gctgatgccg taggcggcat
     1321 tccctctact gcgttgaaag gcgcggggct gttaccgcac atccgcgcga ccgtaaagtt
     1381 gccgatgatt gttatgaagc gcacgctaca gcagctcctg ggcggcgcac cgtttattat
     1441 cagcggtgcc tgcggaatgt tccgtactga tgtattgcgt aagttcggtt tctcggatcg
     1501 tactaaagtc gaagaccttg atctcacctg gacattagtg gcaaacggct accgtattcg
     1561 gcaggcgaat cgctgcatcg tatacccaca ggaatgcaac agcccgcgtg aggagtggcg
     1621 tcgctggcgg cgttggattg tgggatacgc ggtctgtatg cgcctgcata aaagactttt
     1681 atttagccgc ttcggtatct tcagtatatt tcctatgctg ttggttgtgc tttatggcgt
     1741 tgggatttat ctcactacct ggtttaatga attcatcacc accgggccgc atggtgtggt
     1801 gttggcaatg tttccgctta tctggatcgg cgtagtttgt gttattggtg cttttagcgc
     1861 ctggtttcat cgttgctggt tgttggtgcc tttagcgccg ctttccgttg tgtatgtatt
     1921 attagcttat gccatctgga ttatttatgg acttattgcc ttttttactg gacgcgaacc
     1981 tcagcgcgac aaacccaccc gctattccgc actggtggaa gcgtcaaccg cttattccca
     2041 accttctgtc acaggaactg aaaaactatc tgaagcttaa tgttaaagag aaaaatattc
     2101 agattgcaga ccaggtaatt attgatgaaa ctgcaggtga agttgttatc ggcgcgaata
     2161 cccgtatttg tcatggtgcc gttattcagg gtccggtagt gattggcgca aactgcctga
     2221 taggtaatta tgcgtttatt cgtcctggca caataatcag caatggtgta aaaattggtt
     2281 ttgccaccga aattaaaaat gcggttattg aagcggaagc aacgattggt ccgcaatgtt
     2341 ttattgccga ctcggtagtt gcaaaccagg catatttggg cgcacaagta cgtaccagta
     2401 atcatcgtct ggatgaacag cctgtgtctg ttcgaactcc agagggaatt atcgctaccg
     2461 gatgcgataa attaggttgt tatatcgggc agcgttcacg ccttggcgta caagttataa
     2521 ttttgcctgg gcgaattatt tccccgaaca cgcaacttgg cccgcgcgtg attgtagaac
     2581 gtaatttacc tgccggaact tattcactcc gacaagaact tatccgtaca ggagattaaa
     2641 atgataaaac ggacgttatt agcggcggcc atttttagcg cattgcccgc ttatgccggg
     2701 ttaacttcca ttaccgcggg ctacgatttt accgattatt ctggcgatca tggcaaccgt
     2761 aatttagcgt atgctgaact ggtggcgaaa gttgaaaacg caacgctgct ttttaatctt
     2821 tcccaggggc gtcgtgatta tgaaactgaa catttcaatg ccactcgcgg tcagggtgcg
     2881 gtctggtata aatggaataa ctggctgaca acccgaacgg gtattgcctt tgcggataat
     2941 acgccggtct ttgcccgcca gaattttcgt caggatatta acctggccct gttgccaaaa
     3001 acgcttttca cgaccggtta tcgctacact aaatattacg atgatgtcga agtcgatgcc
     3061 tggcaaggcg gcgtatcact ctatactggc ccggtgatca ccagctaccg ctatacccat
     3121 tatgactcca gcgatgcagg tggtagttat agcaatatga tttccgtgcg cctgaatgac
     3181 ccgcgcggca ctggttatac gcaactatgg ctaagccgcg gaacaggcgc ttacacctat
     3241 gactggacgc cagaaacacg ctacggcagc atgaagagcg tcagtctgca acgtattcaa
     3301 ccgctgactg agcaacttaa tctcgggctg acggcaggta aagtgtggta cgacacccca
     3361 accgatgatt ataacggtct gcaacttgca gcccatctga cctggaaatt ctgattcctt
     3421 ctgccgcccg ctatccgggg cggccttccc tgccgattag cccccccttt cctctttgtt
     3481 ttccgaccac attcaccgga taaattttat tctccagtgt tatatactat aggggggtat
     3541 gcattgacat gtagtatacc cccctatagt atattgcgtg cagatgatga ggtgcgaaat
     3601 gcccagtact ccggaagaga agaaaaaggt ccttactcga gttcgtcgta ttcgagggca
     3661 gattgatgct ctggaacggt cgatggaggg tgatgccgaa tgccgtgcca tactccaaca
     3721 gatcgctgcc gttcggggcg cggctaacgg gctgatggca gaagtgcttg aaagccatat
     3781 ccgggaaacg tttgaccgaa atgactgcta cagccgcgaa gtcagccaat ccgttgacga
     3841 cactattgaa ctggttcgag cctatcttaa atagctgaat ctattaccat attgaggaag
     3901 agcgagagat gaaatcacgt gctgccgttg catttgctcc cggtaaaccg ctggaaatcg
     3961 ttgaaattga cgttgcacca ccgaaaaaag gtgaagtgct gattaaagtc acccataccg
     4021 gcgtttgcca taccgacgca tttaccctct ccggggatga cccggaaggt gtattcccgg
     4081 tggttctcgg tcacgaaggg gccggcgttg tggttgaagt cggtgaaggc gtaaccagcg
     4141 tcaaacctgg cgaccatgtg atcccgcttt acaccgcgga gtgcggcgag tgtgagttct
     4201 gtcgttctgg caaaactaac ctctgtgttg cggttcgcga aacccagggt aaaggcttga
     4261 tgccagacgg caccacccgt ttttcttaca acgggcagcc gctttatcac tacatggggt
     4321 gctctacatt cagtgaatac accgtggtcg cggaagtgtc tctggccaaa attaatccag
     4381 aagcaaacca tgaacacgtc tgtctgctgg gctgtggcgt gaccaccggt attggcgcgg
     4441 tgcacaacac agctaaagtc cagccaggtg attctgttgc cgtgtttggt cttggcgcga
     4501 ttggtctggc agtggttcag ggcgcgcgtc aggcgaaagc gggacggatt atcgctatcg
     4561 ataccaaccc gaagaaattc gatctggcgc gtcgcttcgg tgctaccgac tgcattaacc
     4621 cgaatgacta cgacaaaccg attaaagatg tcctgctgga tatcaacaaa tggggtatcg
     4681 accatacctt tgaatgcatc ggtaacgtca acgtgatgcg tgcggcgctg gaaagtgcgc
     4741 accgcggctg gggtcagtcg gtgatcatcg gggtggcagg ttctggtcag gaaatctcca
     4801 cccgtccatt ccagttggtc actggtcgcg tatggaaagg ttccgcgttt ggcggcgtga
     4861 aaggtcgttc ccagttaccg ggtatggttg aagatgcgat gaaaggtgat atcgatctgg
     4921 aaccgtttgt cacgcatacc atgagccttg atgaaattaa tgacgccttc gacctgatgc
     4981 atgaaggcaa atccattcga accgtaattc gttactgatt tcccgcaggt ataccccgtc
     5041 cacttcagac ggggttctta atactctccc tgggcagccg tccgggggat taaccgtgag
     5101 ataatgactg atggaactca ttgaaaaaca tgccagcttt ggcggctggc aaaatgtgta
     5161 tcggcattat tcccaatcac tgaaatgtga aatgaatgtc ggcgtctatc tcccaccaaa
     5221 agccgcgaat gaaaaattgc cggtgctgta ctggctttcg ggcctgacct gcaacgagca
     5281 gaatttcatt actaaatcgg ggatgcagcg ttacgcggct gaacacaaca ttattgttgt
     5341 tgcgccggac accagtccgc gaggcagtca tgtcgcagat gccgaccgtt acgatctcgg
     5401 gcaaggtgcc gggttttacc tgaacgcaac gcaagcgccg tggaatgaac attacaaaat
     5461 gtatgactat atccgcaacg agctgccgga tttagtgatg caacattttc cggcaacgac
     5521 cagaaagtct atctctggtc attctatggg cgggctgggc gcgctggtgc tggcgttacg
     5581 taacccagat gaatatgtca gcgtctcggc gttttcgccc attgtctccc catcgcaagt
     5641 accgtgggga cagcaagcct ttgccgcata tcttggtgaa aataaagatg cctggttgaa
     5701 ttacgatccg gtgagtctta tttcacaagg tcaacgcgtt gcggaaatca tggttgatca
     5761 ggggttgagt gatgattttt acgcggaaca gctgcggact ccaaatcttg aaaagatctg
     5821 ccaggagatg aatatcaaga cgttaatccg ttatcacgag ggttacgacc acagctatta
     5881 ttttgtctcc agttttattg gcgagcatat tgcctaccac gccaataaac tgaatatgcg
     5941 ttgataatag tgcacgactg ccggatgcgg cgtgaacgcc ttatccgcct acatttcgcc
     6001 cgcaggccgt aggcatgata agacgcgcat agcgtcgcat caggcatctg cgcacgactt
     6061 atctatgttt accacatcaa atcatcaggc actttgaagt cggcatatgg gtcgtcttca
     6121 tcctgctctt cggcgctgag cgcgctgtgc aacactatac tgctggcatc acgctgagct
     6181 attttatcgg caacgctggc gggaataatg gcgtattcac cttcgctatt gttatcgacc
     6241 aacaagcgag caatcgccag acgaccatta atcaactgcg cctgtgtgag cttatcgaca
     6301 aaaatcttct taatcagatt gccgtcagtg aagttaaaac caatatcgcc attggcaatg
     6361 gtgattcggt tcatttcaat gagctgcttc acctgagctt tatattcttt cgccaacgca
     6421 gcttgttttt gttgctcgct aagctgttta tcacgctcaa gctgtgcctt tttattttct
     6481 tctaccgccg cccgagcttc acgcgcctga acgcgcgatt ttttcgccgt tctctccacc
     6541 ttcgccgctt ttttgctggt gactaatcct gctttgagca actgctcttg taaggtaagt
     6601 tttgccatct ttgtttccaa acccgctgaa taatttttag cgagtatacc tgtaaacgcc
     6661 gtgggtgtat tgggggcaac tccctttcgc ccctgcttaa taaggcgcgt caggcatcgg
     6721 cacacggctg cattcttgat ctccgactca tcaaaataaa caccgccaac ccagcaacaa
     6781 gaatacccgg tgcagaagcg gccattacgc cgaccgtgcc agtgcctaat gccagcattt
     6841 tcccggccag taacggaccg ctcatagccc ccagacgccc taccgccacg gctgttccca
     6901 cacctgttgc gcggatctgc gaactgtaaa acaacggtgc cagggcatac aaaacgcttt
     6961 gcccacctgt cgcaaacaac cccgcgacaa atcccgccag caacataccg ttaaatgacg
     7021 acaccgttcc aagtgccagc agcgaagcta acatgccgct ataaatcagt agcgacatgg
     7081 tcactggacg cagcttatcc atcaatgcgc ccaacattaa cgtcccgctt gccgcaccca
     7141 tttgcagggc aaacatcacc cctgccgcct gcgatggctg gaatccttgc tccaccaaaa
     7201 gcagcggtag ccagttgatc aacatgtaga ccaccagcag agtgaagaaa taacacaacc
     7261 acagcagcag cgtcgcggtt gccgtttctg gcgcaaataa ggcacgcagt ggtggcgcag
     7321 cctgtttatc gccagcgaaa accgccgact ccggcagcca gcgcattaat agcggcacca
     7381 gaatcaacgg caccacacca cctacccaaa acaccgtttg ccatgctaag tttgcccccg
     7441 cgaaacccag tgtcgccgcc agcgccgcgc caatgggaac accgcaatac atcaggctca
     7501 ctgctgtccc acgaaaacgt ggacccgcgg cttcagacgt cagggcgata agattcggca
     7561 acgccgcccc cagcccgaca ccggtcatca gccgcgcaaa gaccagtgag gggaaatccc
     7621 aggcaatcgc cgttgccagc gagaacaaac caaacagcgc aactgagcca atcaaaatgc
     7681 gcttgcgacc ataacggtcc gccagcattc cgccaaccaa cgcgccgggt agcaaaccga
     7741 gtattccggc gctgaatatc cagcccattt gcattttatc gagtgcgaaa gcctgggcga
     7801 tgccacccgc cgcaatgcca gccgcctgaa gatccagccc ttccatcaga gcgaccaaaa
     7861 aacaaagccc gatagtcagc atcaggcggg atgaagatga tgaaggggta cgagtcgaca
     7921 taagatgaac ctcgttgcga gtgcaggtga atcgcgactg atacattgcc agccgcgtaa
     7981 tcagaactat ttaatgactg cctacgggcg aaatgtaggc cggacaagac gtttatcgca
     8041 tccggcagtc gtgtgcagat gcctgatgcg acgctaacgc gtcttatcag gcctacaaat
     8101 tctcacgcca cccgtaggcc ggataaggtg tttacgccgc atccggcagt cgtgctggga
     8161 tgggcataca aaatgcacgt tctcggcagt catcattatt tgttgttgcg cagatccagc
     8221 gccacgtcaa cgatcatatc ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg
     8281 agaatatcca ccgcacttaa gccataacgc gccgccgccg tttcacagtg acgcaggaag
     8341 ctcgagtaaa caccagcgta tcccagcgcc agcgtttcgc gatcgactcg taccggtcga
     8401 tcctgcaacg gacgcaccag gtcgtcagcg gcatccatta acgcatagag atcggtccca
     8461 tgctgccagc ccagtttatc cgcggcggca ataaatactt ccagcggcgc gttacctgcg
     8521 cccgcgccca ttcccgcgag gctggcgtcg attcggtcgc agccctcttc caccgccgcg
     8581 atagagttcg ccacgccaag actcaggtta tggtgagcgt gcatgccagt ttgcgtttct
     8641 ggtttcagct ctgctttcag ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca
     8701 ccgccagaat ccaccacata aatacaggtc gcaccgtagc cttccatcag ctttgcctgc
     8761 tttgcgagat tctccggcgt ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt
     8821 ccgagctcgc gggcatactg aatatgctgg gcggaaacat cagcttcggt acagtgcgtt
     8881 gccacacgaa ccacccgcgc gccagcctgc caggcatttt tcagatcgtg aatagtgccg
     8941 attcctggca gcaacaacgt cgcgattttg gcgtgcttca ccacatccgc cgctgcttca
     9001 atccattcaa ggtcgctatg tgcgccgaaa ccatagttaa agctggaacc ctgcaaaccg
     9061 tcgccatggg ccacttcaat cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg
     9121 cgaacgtttt ccagcgaata ctgatgacga atggcgtgca taccgtcacg caacgtgacg
     9181 tccgagatat aaagtttttt accgttcatg ccgcttctcc tgccttgcgc gccagtgact
     9241 gggccatttt ttccgctgtc gccagcgcac tggaagtcat aatgtcgagg ttgcccgcat
     9301 aggcaggcag ataatgcgct gcgccttcga cttccagcca gaccgctgtt ttcagtccgg
     9361 agaattgccc cacgcccggt aaattgaccg gtttatcctg cgggataact tcaaactgca
     9421 cgcgctgttt caggcgataa cccggtacgt aagcctgcac cgcctcagcc atttcattga
     9481 ttgaggcttc gatatcatct tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca
     9541 tcatcaacgg tggctctgct gggttaagca caataatcgc cttccctttt gccgcgccgc
     9601 ccaccacttc aatggctcgg gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc
     9661 caggtccggc agatttactg gcgatagaag cgataatttc ggcgtaatga acacgcgcca
     9721 cgcgtgaaac tgccgccacc attggaatgg tggcctggcc gccgcaggtg accatgttga
     9781 cgttcagttg atcgacgttc gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg
     9841 cagcaggcgt caggtcaatt aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt
     9901 ttttcacatg cgcgcccgcg ctggtcgcat caaatacaat gtcgatatca gcaaattcag
     9961 gcatgttcat cagcccgatc accccttcat gggtggtggc gacgcccata cgtctggcac
    10021 tcgccagacc gtcggactga ggatcaatgc cgaccatcac cgccatctcc agatgctgac
    10081 cgtgacgcaa aattttaatc atcagatcgg tgccaatgtt gccagaaccg ataatggcga
    10141 ctttacgctt actcatgaca gacttccttt tggggctgcg cttgaaaatg tcgcagcaac
    10201 tgaacctatg ccttcaatat gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg
    10261 gcctaatgcc ccggtaagaa tgatatctcc ggcgcgcagc ggttcaccca gactggccat
    10321 tttgcgtgcc agccagacgg cggcattaag cggatgtccc aggcattcgc tgccgcgccc
    10381 gctggaaacc tcttcgttat tacgcgtcat cttcatggcg cagtttttca ggtctaaccc
    10441 cgccggacgt tgcgccggac cgccgatgac atacaccccg caggaagcgt tatctgccac
    10501 ggtatcgaca aactgaatcg accagtcgcg aatgcggctc cccaccactt ccagcgccgg
    10561 aagtacccat tcaatggcgt tatacagttc gtcgaaggtg atatcagttg cgggtaaatc
    10621 gcggttcaac accagtgcga tctccgcttc aatgcggggt tgcagaacac gggaaaaagg
    10681 aatgatttcg ttatcgccat aacacatgtc ggcaaataac gtcccaaaat ccggttgatc
    10741 aacgcccagt tgttgttgca ctttcggatg cgtcaggccc actttacgcc ctaccacgcg
    10801 acgcccctgc acaacgtcat attgcacatt tatgtgctga atggcgtagg ccgcttcagc
    10861 gttatcgata ccaatcagat cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg
    10921 gcgtaaatcc gccgccagtt gctcaagagt atgcttcgtc atgaccattc cttaagggcg
    10981 tgcgaggaaa ttcagcacca attgattgaa agcgtcggca tgttcccact gcgcccagtg
    11041 accacagtcg cggaagatat gcagttcaga accggcaatg ccggacagca gacgcagacc
    11101 cgcatccatc ggcacaaagc ggtcgttgcg cccccagaca atcagggttt gcgctttgat
    11161 ttccgccaga cgtgggccaa aatccgggaa ctgtttcgga ttagcttcca ggctcttaac
    11221 gaagttttcc tggtgatcgc ggcgcgacag catattattc aggcgcgctt caaacagggc
    11281 gtcggtcaaa tcgctggtat cgaaaacgaa gatatccatc atcagcttca ggttttcgat
    11341 agtcggctga cgataaagct gattcagtcg cttaatacct tcggttggca tcggcgtaaa
    11401 caaactcatg ccgcccgtac cgccgcccat cagcaccagt ttgccgacgc gctccggcca
    11461 gttaagggtg aacgccacag aactatggcc gcccatcgag ttgcccagca ggtggatttt
    11521 ggcgatatcc agttgatcca ccacgctttt caggattcgt gcattaagat ccgatcgtga
    11581 accactatta acgatcgaat cgcttttgcc ccaacccgga caatccagca ggattacccg
    11641 atagcccgcc tctaccagcg gatcgatatt gcggctgaag ttcgcccagc cagtagcacc
    11701 cgggccggaa ccatgcagca ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg
    11761 gatgcgcagc gttttacccg cttcttctac attcagaaaa cggctggtgg cggcttcggt
    11821 ttgtggctga taactcatca tcttctcctg catattcagt tctctgttct ggcgcttaac
    11881 gagccaaatc cggcaatcca ctccgggatt gggcggtaat aacgcccttc gctacgccag
    11941 ttgccaaatg tagaaatagc ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac
    12001 tttccggcaa tggcggaaag ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc
    12061 tgctccagca aagtcatgaa ctggttatcc caaatcgggt tgagcggatg cagagttctc
    12121 tgatcctcaa caaacttctc agcggcgcta atcacccgtt gctgacgcaa ttcgcgctca
    12181 ctggcgggta aatctttccc gctccccaac agacggtcgc gcatatgggc atcggctttc
    12241 gccagttcgg gcaccggcgg ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt
    12301 ttattgagag tgctggtgaa acgtccaatg gcttcaccca acatgcgggt acgctggaaa
    12361 ccgggcagcg gcgtggcgac accgttgatg aacacaggca gaactggcac cttatccagc
    12421 ccaccgagca ggaactccag cggctgggcg aacccgtggt ccacctgcat acagtaagaa
    12481 acggcaagat cgatcccgct cttcatgaca gcatgcgcac aggcctccgc cagctccaca
    12541 ggcacgggca gctctcctgc cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct
    12601 aaacagaacg gtggcatcac gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc
    12661 accaccagtt caggggagaa agccgcaata cgctcgcggg cgctggcaat cacgccattg
    12721 acctcatcga gtacctcttg cgccgggtcg acatacccca ccaacggcga gtgggaaaga
    12781 cagtgaagat aagcgtgcat atcaggctac cttttcgaca gaaacgtcgg catcagggcg
    12841 ggtcagcgtc atcaccgacg ccagtttatt cagcgtattg cccagggttt gcggaatggc
    12901 ggtggcggca acaaagcgat ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa
    12961 ccagctacgc agacgacctt gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg
    13021 tgcggtatga atttgcactt ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca
    13081 ctgctggatt tgctcgtcgc tcatccccca cagtggattg catccccagc caattaccgc
    13141 gaagttcgcg ccaatcgcgt tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt
    13201 cggctgaata aacatcttgc cgaccggaga gtgcttcgct tcgccctcac gtaccagcgc
    13261 accgccgtaa tattgcggca tcggcttgaa gcgcatttcg aggaagtagc gttttactgg
    13321 cggcagataa ttcagtagcc aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg
    13381 cggagccagc acgttgcccg ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg
    13441 acgttcttgt tgataggtat cgagcagcac atcgcgggct ttcccctgga taaccaacgc
    13501 cagtttccag gcgaggttaa aggcgtcgcg cataccactg ttatagccct gcccctgcca
    13561 caccggcatg atgtgcgcgg catcgcccgc cagcagtacg cgatcaatac ggaaacgttg
    13621 cgccagtcgc gcgttatggg tgtagacacg ctggcgaatc aattcaacat tgtccggatt
    13681 aggcagcact ttgcttaaca gcttgcgcat attttgcggc tcacgcagct gctcttcggt
    13741 ttctcccggc atcaccataa attcaaagcg acgtaccgca tgaggcagcg cggcagaaac
    13801 atacgggcgc accggatcgc aacacaaata gatatgcggc gtacttaacg gatcgttggc
    13861 gatatctacc acaatccact gatttggcgc agttttacct tcaaacggca cattcagagt
    13921 gcgacggaca aaacttgctc caccgtcaca ggctaccagc cactgggctt tgactatttc
    13981 ccgctgccct tctgccgttt tcaggtgcaa ggtcacttcg tcattttgct ggctgaaggc
    14041 ctccagctcg cgggaaaaca agcagcgcac attaggaaaa cgcgacaccc cttccagcat
    14101 caccgcatcg acctgcggct gaataaaggc gttacggcgc ggccagccaa attcatcggt
    14161 cattggctga atatcagcaa aacagcggcc tttcggggtg agaaaacgca tcgcgtgcca
    14221 cggcgtagtg tgcggcagaa cattctcgac caggccaacc gactgcatgg tgcgcagcgc
    14281 ctcgtcatca ataccaatcg cacgcgggta gtcgatcaac ttatcgagtt tctccaccac
    14341 cagcacgtca atgcccatct gaccgagata gttcgccatc atcagcccga ccggaccggc
    14401 acctgcgatc gccacctgaa cgctatggtt aacagcaggc tggatgtcag ggtgttgtat
    14461 tgccatttca gtacctcacg actcggacaa aatgccgttg cgcgcacagt acggcgcaac
    14521 ttattttgtt aaaaacatgt aaatgatttt ttattgtgcg ctcagtatag gaagggtgtt
    14581 ttcggctaca atcaaaacat gcccgaatgt gcaccaggtg cacaacgttg ttttaactat
    14641 agaaatgtca attaatatgc agaacaatga gcagacggaa tacaaaaccg tgcgcggctt
    14701 aacccgcggt ctaatgttat taaatatgtt aaataaactt gatggcggtg ccagcgtcgg
    14761 gctgctggcg gaactcagcg gcctgcatcg caccactgtg cggcgactgc tggagacgct
    14821 gcaggaagag ggatatgtcc gccgtagccc ctccgatgat agttttcgac tgaccatcaa
    14881 agtgcggcaa ttaagcgaag gatttcgtga cgaacagtgg atttctgcac tggcggcccc
    14941 actgctgggc gatctgttgc gcgaagtggt atggccgaca gatgtgtcca cgctggatgt
    15001 tgatgcaatg gtggtacgcg aaaccactca ccgtttcagc cgcttatcct ttcaccgggc
    15061 aatggtcggg cgacgtttgc cgcttctgaa aaccgcctcg ggcctgacct ggctggcctt
    15121 ttgcccggaa caagaccgca aggaattaat cgaaatgtta gcctcccgcc ccggtgatga
    15181 ctatcaactg gcacgggaac cgttaaagct ggaagccatt ctggcgcgcg cgcgcaaaga
    15241 gggttacgga cagaactacc gcggctggga tcaggaggag aagatcgcct ctatcgccgt
    15301 accgctgcgc agtgaacaac gggtgattgg ctgtctgaat ctggtgtata tggcgagcgc
    15361 aatgaccatt gaacaggcag cggaaaagca tcttccggcg ctacaacggg tagcaaaaca
    15421 gatcgaagaa ggggttgaat cgcaggctat tctggtggcc ggacggcgaa gcggcgtgca
    15481 tttacgttga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgttcgg
    15541 aagagagtca attcagggtg gtgaatgtga aaccagtaac gctatacgat gtcgcagagt
    15601 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
    15661 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
    15721 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
    15781 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgctgat caactgggtg
    15841 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
    15901 acaatcttct cgcgcaacgc gtcagtgggc tgctcattaa ctatccgctg gatgaccagg
    15961 atgccactgc tgtggaagca gcctgcgcta atgttccggc gttatttctt gatgtctctg
    16021 accagactcc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
    16081 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
    16141 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
    16201 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
    16261 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
    16321 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
    16381 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
    16441 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
    16501 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
    16561 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    16621 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    16681 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tgaaattgtg
    16741 agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt
    16801 cgttttacaa cgtcgtgact gggaaaaccc tggcgttaca caacttaatc gccttgcggc
    16861 acatccccct ttcgccagct ggcgtaatag cgaagaagcc cgcaccgatc gcccttccca
    16921 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
    16981 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa
    17041 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt
    17101 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt
    17161 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc
    17221 gtttcatctg tggtgcaacg gtcgctgggt cggttacggt caggacagtc gtttgccgtc
    17281 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct
    17341 gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt
    17401 ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac
    17461 tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga
    17521 gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag
    17581 cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtagttatg ccgatcgcgt
    17641 cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta
    17701 tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga
    17761 tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt
    17821 gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga
    17881 gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg
    17941 ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt
    18001 ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga
    18061 tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa
    18121 tcacccgagt gtgatcatct ggtcgctggg gaatgagtca ggccacggcg ctaatcacga
    18181 cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg
    18241 cggagccgac accttcgcaa ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga
    18301 ccaacccttc ccggcggtgc cgaaatggtc catcaaaaaa tggctttcgc tgcctggaga
    18361 actgcgccca ctgatccttt gcgaatatgc ccacgcaatg ggtaacagtc ttggcggttt
    18421 cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga
    18481 ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt tggcttacgg
    18541 cggtgatttt ggcgatacgc cgaacgatcg ccagttctgc atgaacggtc tggtctttgc
    18601 cgaccgcacg ccgcatccgg cgctgacgga agcaaaatac cagcagcagt ttttccagtt
    18661 ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa
    18721 cgagctcctg cactggatgg tggcgctgga tggcaagccg ctggcaagcg gtgaagtgcc
    18781 tctggatgtc gctccacaag gtaaacagtt gattgaactg cctggactac cgcagccgga
    18841 gagcgccgga caactctggc ttacggtaca cgtagtgcaa ccgaacgcga ccgcatggtc
    18901 agaagccgga cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagcgtgac
    18961 actccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat
    19021 cgagctgggt aataagcgtt ggcaatttaa ccgtcagtca ggccttcttt cacagatgtg
    19081 gattggcgat gaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct
    19141 ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg
    19201 ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgct cggcagatac
    19261 acttgccgac gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg gaaaaacctt
    19321 atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga
    19381 tgttgaagta gcgagcgata caccgcatcc ggcacggatt ggcctgacct gccagctggc
    19441 gcaggtcgca gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg
    19501 ccttactgcg gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta
    19561 cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca
    19621 ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga
    19681 aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt
    19741 ccatatgggg attggtggcg acgactcctg gagcccgtca gtgtcggcgg aattccagct
    19801 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag
    19861 gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa aaaacacaaa
    19921 cttttggatg ttcggtttat tctttttctt ttactttttt atcatgggag cctacttccc
    19981 gtttttcccg atttggctac atgacatcaa ccatatcagc aaaagtgata cgggtattat
    20041 ttttgccgct atttctctgt tctcgctatt attccaaccg ctgtttggtc tgctttctga
    20101 caaactcggg ctgcgcaaat acctgctgtg gattattacc ggcatgttag tgatgtttgc
    20161 gccgttcttt atttttatct tcgggccact gttacaatac aacattttag taggatcgat
    20221 tgttggtggt atttatctag gcttttgttt taacgccggt gcgccagcag tagaggcatt
    20281 tattgagaaa gtcagccgtc gcagtaattt cgaatttggt cgcgcgcgga tgtttggctg
    20341 tgttggctgg gcgttgtgtg cctcgattgt cggcatcatg ttcaccatca ataatcagtt
    20401 tgttttctgg ctgggttctg gctgtgcact catcctcgcc gttttactct ttttcgccaa
    20461 aacggatgcg ccctcttccg ccacggttgc caatgcggta ggtgccaacc attcggcatt
    20521 tagccttaag ctggcactgg aactgttcag acagccaaaa ctgtggtttt tgtcactgta
    20581 tgttattggc gtttcctgca cctacgatgt ttttgaccaa cagtttgcta atttctttac
    20641 ttcgttcttt gctaccggtg aacagggtac gcgggtattt ggctacgtaa cgacaatggg
    20701 cgaattactt aacgcctcga ttatgttctt tgcgccactg atcattaatc gcatcggtgg
    20761 gaaaaacgcc ctgctgctgg ctggcactat tatgtctgta cgtattattg gctcatcgtt
    20821 cgccacctca gcgctggaag tggttattct gaaaacgctg catatgtttg aagtaccgtt
    20881 cctgctggtg ggctgcttta aatatattac cagccagttt gaagtgcgtt tttcagcgac
    20941 gatttatctg gtctgtttct gcttctttaa gcaactggcg atgattttta tgtctgtact
    21001 ggcgggcaat atgtatgaaa gcatcggttt ccagggcgct tatctggtgc tgggtctggt
    21061 ggcgctgggc ttcaccttaa tttccgtgtt cacgcttagc ggccccggcc cgctttccct
    21121 gctgcgtcgt caggtgaatg aagtcgctta agcaattaat gtcggatgcg ccactgggca
    21181 catccgacaa ttatttatag ctccatcatc tggcgaaaca agcgtcgcat catacctctt
    21241 tgacccacag ctgcggaaaa cgtgctggtg caaaacgcag ggttatgatc atcagcccaa
    21301 cgacgcacag cgcatgaaac gcccagtcca tcaggtaatt gccgctgata ctacgcagca
    21361 cgccagaaaa ccacggggca agcccggcga tgataaaacc gatcccctgc ataaacgcca
    21421 ccagcttgcc agcaatagcc ggttgcacag agtgatcgag cgccagcagc aaacagagcg
    21481 gaaacgcgcc gcccagacct aacccacaca ccatcgccca cgataccggc aattgcagcg
    21541 gcaaccagat aaagccgcag aaccccacca gttgtaacac cagcgccagc attaacagtt
    21601 tgcgccgatc ctgatggcga gccatagcag gcatcagcaa agctcctgcg gcttgcccaa
    21661 gcgtcatcaa tgccagtaag gaaccgctgt actgcgcgct ggcaccaatc tcaatataga
    21721 aagcgggtaa ccaggcaatc aggctggcgt aaccgccgtt aatcagaccg aagtaaacac
    21781 ccagcgtcca cgcgcgggga gtgaatacca cgcgaaccgg agtggttgtt gtcttgtggg
    21841 aagaggcgac ctcgcgggcg ctttgccacc accaggcaaa gagcgcaaca acggcaggca
    21901 gcgcccacca ggcgagtgtt tgataccagg tttcgctatg ttgaactaac cagggagtta
    21961 tggcggcacc aagtccaccg ccgcccatca gagccgcaga ccacagcccc atcaccagtg
    22021 gcgtgcgttg ctgaaaccgc cgtttaatca ctgaaggcat caccgcctga atgataccga
    22081 tccccacccc accaagcagt gcgctgctaa gcagcagcac actttggggg taaagctcac
    22141 gcatcaatgc accgacggca atcagcaaca gactgatggc gacactgcga cgttcgctga
    22201 catgctgatg aagccagctt ccggccagcg ccagcccgcc catggtgacc accggaagag
    22261 cggtcaacag ggcagccacg ctaaagctca ttccgctcgc ctggcgcaat tgcggtagca
    22321 gtggcccgac ggaggtgagc agtggtcgca tattaagacc aatcagcacc agaaccagca
    22381 gcatcgtgaa cgtcttttga tgattgcgca tggctgtcag aacggcttgg tcggcagata
    22441 tttaccatct aaggtaatga ccgcacgttc gccaccttcc gggtccgcca ctttcttaac
    22501 gtcgagtttg aagttaatcg cgctgataat gccatcgcca aacttctcat gaaccaacgc
    22561 tttcagggtt gtgccgtaca cctgcaacat ttcatagaaa cgatacatcg ttgggtcggt
    22621 tggaatacgg tcatcaatgc agccacgcag tggaatcatc tgcaacagta gaatggcgtc
    22681 ttcgtcgaga tccagcttcg ccccgaccag gcgggcggcg tcggcaggaa gcgcttgctg
    22741 ccccagcaaa gccgcggtta cgaaggcttc tgccagaccg gtaccgtcgg caatctcggc
    22801 aaatgagaga tcttttttag ctttgctgag caaaatggca tcggcaagat caagacgaat
    22861 attgcggtta atttgtgact gaatcatggt ggatctcctg atggtttaaa aataaggtta
    22921 cgctgcggtc ggttggcgta gcggtatggc acaaacgcga ggattagcgg ccagtggcac
    22981 aaactggcgg gttgcgccgt caaaagctgc gatgctgccg ctttcaatgt cgtagaccca
    23041 gccgtgcagg gcgatccgcc cctcttcgag cgccaggcgc accgatggat gagtttgcaa
    23101 attagccaac tgagcaatga cgttttcacg taccatcgcc gcagcttttg acggtaaatc
    23161 ggaatgcggg cgcgcctcat taacgacgcg ggctgaatcg gcataacgca gccagtggga
    23221 gacggcaggc atatggtcca tgcactgaca gctggcaatg gcggtcatcg cgccacagtt
    23281 ggaatgacca caaatcacaa tgtcagatac ccgaagcgca gcgacggcat actccaccga
    23341 agcagaaacg ccaccgggtt ccggcccgta ggaagggacg atattgcccg cgttgcgaat
    23401 aacgaacaga tcgccaggct cacgttgcgt caccagctca gggaccagac ggctgtcgga
    23461 gcaggagata aaaagtgtgc gcgggctttg ctgtgtcgcc agctgtttaa acaaggcttc
    23521 ccgcttcgga aatgcctcgc gctggaattt aaggaatcca tcaataatct ctttcaccgt
    23581 taacctctgt ctgtctctgg aatgagaggc agactactcg ccgattgtca taaggtaaaa
    23641 gtctcattta tgatgagttc tattggattt acttataggt tggcgaatgc tctctcgaca
    23701 tatcaattat tttcttgccg tggctgaaca tggcagcttc acccgtgccg ccagtgcgtt
    23761 gcacgtctct cagcctgcgc tttcccagca gattcgccag ttagaggaga gtttaggcgt
    23821 gccgctgttt gaccgtagcg ggcgaacgat tcgtctcacc gatgcaggag aagtctggcg
    23881 acagtacgcc agccgggcgt tacaggaact gggagcgggt aaacgggcga ttcatgatgt
    23941 tgccgatctg acgcgaggat cgctgcgtat cgccgtcacc cccaccttta caagctactt
    24001 tatcggccct ttaatggcgg atttctatgc gcgctacccc ggcatcacgc tccagctaca
    24061 ggaaatgtcg caggagaaaa tcgaagatct gctttgccgc gacgagctgg acgttgggat
    24121 tgccttcgcg cctgtgcatt cgccggagct ggaggcaatt cctttactga cagaaagttt
    24181 agcgttagtc gtggcgcaac atcatccgct ggctgcctgt gaacaggtgg cgttgagtcg
    24241 cttgcatgat gaaaaactgg tcctgctcag cgcggaattt gccaccagag agcaaattga
    24301 ccactactgc gagaaagcgg ggctacatcc acaggtggtc attgaggcga actcaattag
    24361 cgcggttctg gagctgattc gccgcacttc cctttccaca ttgttaccag cagcgattgc
    24421 cacacaacat gacgggctta aagcgatttc tcttgccccg ccactactgg agagaacggc
    24481 ggttttgttg cggcggaaaa atagctggca gacagccgcc gcgaaggcat ttttgcacat
    24541 ggcgttggag gaatgtgcgg atgttggcga gaatgaatca cggtagccac tgacacaggt
    24601 cagtggctct tcaacacaga ggaaattacc gtttgtaatc gatggcttct ggctgctcca
    24661 gatatacggt ggtttgtgcc ggttgtgtac tggcaatcac cttgccgcca cgtaccgaat
    24721 aacgtaccgg aacctgacgg cgcagcgcat caaatccatt ttcagccggc aggataatca
    24781 ggtttgcgct gtttccggcg gcaatgccgt aatcctgcaa attcaatgtt ctggcactgt
    24841 ggtgggtgat taaattcagg ccatcgttaa tctgcccgta gcccatcaac tggcaaacat
    24901 gcagccccat atgcagcact tgcagcatat tcgccgttcc cagcggatac cacggatcga
    24961 agacatcatc gtgaccaaag cagacgttaa tgccggactc cagcatctct ttaacgcgcg
    25021 tgatgccgcg acgttttgga tacgtatcga aacgtccttg cagatgaata ttgaccagcg
    25081 ggttggcgac aaagttaata ccggacattt tcagcaagcg gaacaggcgt gaggtatacg
    25141 ccccgttata ggagtgcatt gccgtggtgt ggctggcggt gactcgcgcg cccatgcctt
    25201 cacggtgcgc cagggcagca acggtttcga caaagcgcga ctgctcgtca tcgatctcat
    25261 cacagtgaac gtcgatgaga cggtcgtatt tttgcgccag ggcgaaggtt ttatgcagcg
    25321 actccacgcc gtattcacgg gtaaattcaa aatgcggaat cgcccccact acatctgccc
    25381 ctaagcgtaa cgcctcttcc agcaacgctt caccgttggg atacgacaaa atcccttcct
    25441 gagggaaggc gacaatttgc agatcaatcc acggcgcgac ttcctgcttc acttccagca
    25501 ttgctttcag cgcagttagc gttgcatccg aaacatcgac atgggtacgc acatgctgaa
    25561 tgccgttggc aatctgccat ttcagcgttt gccaggcgcg ttgtttcaca tcgtcatggg
    25621 ttaataacgc tttgcgctcg gcccagcgtt caatgccttc aaacagcgtg ccggactgat
    25681 tccagttcgg ttgtccggcg gtttgcgtgg tgtccaggtg aatatgcggc tccacaaacg
    25741 gcggtataac taaaccttgt tcggcatcca ggctgttttc agttatgggc atcacgccgg
    25801 attgcgcatc aatggcgctg atttttccgt cctgcagatg aatctgccac agcccctctt
    25861 tgcctggtaa ccgggcgtta ataattgttt gtaaagcgtt attcgacact gttaacctcc
    25921 acatgcgtca ttgctgctgt cgttttacga ttcaaaatcg ggttaaggat cagatagctc
    25981 agcgcgccac ctaataccgc gttgaccgga acaattcccg gtaaccagtg gcctgcggca
    26041 atccccagcg cgaccgccag aatcgccacc caattgacac tcatcatacg cgtggtcgca
    26101 aagtgctcat agcggcgacg gttcatcaga tagtcggcga tgatcacgcc ccctactgga
    26161 ggaatagctg ccgaaaggaa ggtcaaccag ccgacaaaat tgttatacag ccataatgcg
    26221 cagaccgtac cgataatacc gttgattacc gaaagggttt tgctcgacat accggtaatg
    26281 ttggcgaaac ctaaacccga cgcatagagt gcgttatcgt tggtggtcca gatattcagc
    26341 cccagcacca caatcgcagg cagcagcagg ccctgagcaa tcatcacatc agagatatcc
    26401 gccatgccca gtgccgcagc gcccgctgca ccgaaaataa acatcaacga gttgccgagg
    26461 aaaaaggcca ccatcgccac cagcaccgcc agtttggcat tgcgaccaaa ccggacaaag
    26521 tcagcggtga gcgtacccgc actgataaat gaccccacaa ccagcgccag cgcgacattg
    26581 aaatctaacg gttgtgcggg aacgaccgct tttaatgcgt ccaggccgcc catgccgtta
    26641 acagccagcc acacggaata accgcccagg caggcgatag ccggaaccgc aatcaccgaa
    26701 agaaccgtca gcgccgaaat gccaaaaaag acggtgacgg tcatcagtaa accggaaacg
    26761 gcaatcagca aattaatatc cagcccggtt gccttaccca ccggaatggc aaacatcgcc
    26821 acaccgacgc caaaccagcc aacctgagtt ccgcccagta gcagtgaagg cagccatgag
    26881 cctttaacac caaacgagaa gcgagcaaga agatgagtgg tcaggccggt ttttgcgcca
    26941 atgtaaccga gaaatgaagt gtaaataccg aggagaagat taccgatgag aactgcgagg
    27001 aagaaatcat gatagctaag accggttccg agagtgccgc cggtccacat actggcggaa
    27061 aagaaggtta atcccagcat gacgaacgtc aatgccaata cccctttccg cgccgactgc
    27121 gggactggcc cctggctaaa gttgttatct tgcgacacga aattccccct ttttgctgtt
    27181 tgaaacccaa aaaatccgcc gtattctatt catctgaaga taaaaagcaa tcgttttcgt
    27241 ggggaaatat attttttata tgaatgagtg caaaaaggtg ggggaaaaat tatcggtgta
    27301 aaaacagtgg gtagtgggta gatactgata agacgcgtca gcgtcgcatc aggcatcggc
    27361 gcggggtgtc ggatgcgacg cgagcgcctt atccgaccta cggttcaggc ctgtaggcat
    27421 aataagacgc ggtaagcgtc gcatcaggca tcggcgcatg gtgtcggatg cgccacgcga
    27481 cgcatccgac aataccttac tcttccatcg cctggcggat ctgatccaat gacgcaggat
    27541 catcaatggt cgtcagatcc ccaggatcgc gtccttcgca aattgcctgg atcgtgcggc
    27601 gcagcatttt tccggatcgc gttttcggca attgcgagac aaaccagacg tgcgccgggc
    27661 gaccaaagtt gccaatctgg ctgtccacca gcgccataat cgccttctct tgcgaatgcg
    27721 ccacatcacg atcttccaga ctatcgctct ctttcggaat gacaaacgcc accgccacct
    27781 gccctttcag cgcatctttc accccaacca ccgccacttc ggcaacgccc ggatggctgg
    27841 agatactctc ttcaatctcg cgcgtgccca gacgatgccc ggcaacgtta attacatcgt
    27901 cagtgcgccc gagaataaag tgataaccgt cagcgtcacg gatgccccag tcaaaggtgg
    27961 cgtacaccgg gcgggaaaac agcgaccagt aagtcttcac aaagcggtcg tcgtcgcccc
    28021 agatggtctg aatacagccc ggcggcagcg gcccttccac caccagcatc cctttctcgt
    28081 tgacgccaca cggttcgccg gtgacttcat taagcaactg cacgttatag ccatacatcg
    28141 gcacgccggg gcttcccaga cgcgtcggcc tgtcgtccag accgcgagca atcgccataa
    28201 tcggccagcc ggattcggtc tgccagtagt tgtcgatgac cggcacatcc agcgtattgc
    28261 tcacccaact ggcggtcggc tcgtccagcg gttctccagc cagatagagc acttccagcg
    28321 acgagagatc gtgtttgcga atttcagcgg tagggaattt tttcagcacg cgaatggcgg
    28381 tcggcgctga gaacatccgg ctaacctgat atttctcgac gattttccac cacacgccgc
    28441 agtccggcca ggtcggcaat ccttcgtaaa cgatagtcgc catccccgcc agcagcggcg
    28501 cgtaaacgat atacgaatgc cccaccaccc agccgatatc cgatgcgcaa aagaacacgc
    28561 tgcccgcttt gccgccaaaa atggtgtcca tcgaggtcgc cagcgccacc gcatatccgc
    28621 cgacgtcacg ctgcacgcct ttaggtttgc cggtcgtgcc ggaagtgtag agaatgcagg
    28681 aggtttcgtt ggattccagc cacgccaccg gtacccgcgc gccgatgtgt tgatggcgca
    28741 acgacgcgaa atcgacatcc cgcccgctga cgcgcgccat tttcgccagc ccgcgatcca
    28801 ccagcaaaac atggcgtggc tggtgctgcg cctgacttat cgcatcgtcg agcaattttt
    28861 tatagggaat gattttgcca ccgcgcgctc cggcatcagc cgagacaatc agcaccggtt
    28921 tagcgtcatc aattcgcgcc gccacgctgt gcgaggcaaa accaccaaat accaccgaat
    28981 gaatagcacc aatgcgcgcg caggccagca gagtaatatg cgcttcggca atcatcggca
    29041 tatacaccag cacccgatcg ccgcgctgca cgcccagtga gcgcaacatt gaggccaccg
    29101 cgttcacttc gtcatgcagc tggcgaaagg taaaggtgcg ctcttcttct gtttccgaag
    29161 agacggcaat caatgccagt gcctctggct gtttctccag ccagcggtcg atagcgttgt
    29221 ggcacaaatt ggttcggcct tcacaaaacc aacgggcaaa cggcggattg ctgtggtcga
    29281 gcgtttgcgt aaagggcgtc tgccagtcaa tacgccgggc ctgctcggcc cagaactgct
    29341 ccggttcgtt aatcgaacgc tgataaaatt cgctaaaaga cataatgctc tcctgttgaa
    29401 cttacgcctt accgcagttt acttaaatga cgtacaggtc gagatactca ttgaccggca
    29461 tctgttccag gcgagttctg tcgagagaaa cctccagaat gcgctgctgt tggcgagtcg
    29521 ggaactggcg cgcgagattg attttgaatt tatcgaccag tttcggaata ccatcctgac
    29581 ggcggcgagc atgaccaatg gggtactcca ccaccacttc ttcaaatcgt gtgccgtcgg
    29641 tgaactcaag ggtaatggca ttggcgatgg cgcgtttttc cgggtcgtgg tagtcagcgg
    29701 taaatgccgg atcttcaaag caattgatct tctcgcgcag ggcgtcgatg cgtttatctt
    29761 gcgcaacgtt gtcctcgtaa tctgccgccg ttaagcgccc gaataacagc gggatcgcca
    29821 ccatgtactg aatgcagtgg tcgcggtcag ccgggttatt gagcggcccc tttttgtcga
    29881 tgatgcgaat acaggcttcg tgggtgcgaa tggtcacttt ttcgatatcc gccgccgtct
    29941 tgcctgctgc ctgcatctgt tcatagagcg tcatcgccgc ttcaactgcc gtctgggagt
    30001 ggaactccgc cgggaaggag attttgaaca gcacattctc catgacgtaa gaaccgtacg
    30061 gacgctggaa gcggaacgat tcacctttaa aggagacgtc gtagaagccc cataccggcg
    30121 cggtcagggc tgacgggtaa cccatttcgc ccgttttcgc catcagtgcc agacgtaccg
    30181 cgcgggaagt ggcatcgccc gccgcccagg atttacgcgt gccggtgttc ggcgcatggc
    30241 gataggtgcg cagcgactga ccgtccaccc acgccagcga aacggcgttg agaatttcct
    30301 cgcgggtcag gccgagcatt tcggcgacca cggcggtgga agccactttc actaacagaa
    30361 cgtggtcgag gccgacgcgg ttaaaggagt tttccagcgc gatgcagccc tgaatttcat
    30421 gggctttgat cattgcggtc agcacctgtt tcatggtcaa cggcgctttg ccgctggcga
    30481 cagcgttgcg cgaaagccag tctgccgttg ccagaatgcc gccgaggttg tcggaaggat
    30541 gcccccactc cgccgccagc caggtatcgt tgaaatcgag ccagcggatc atcgcgccaa
    30601 tgttaaatgc cgcctggaca gggtcgagct gaaactgagt tccggggacg cgcacgccgt
    30661 tgggtacgac ggtgccagga acaattggcc ccagcagttt tttacaggcc gggtattcga
    30721 gagcttccag accgcagccg agcgtgtcga gcaggcagta atgtgcggtg tcgtaggcca
    30781 ctttagagct gatttcgtag ttcatcacgt aatcgacgat atcaacgatt tcacgatcaa
    30841 attccgggcg gatgttgttg atttgagctg acattgggta tgtttcctta ttgttattcg
    30901 tagaggttta ctggcgctta tccagcgcga caaactggcg gtcttccggt ccaacataat
    30961 tggcggaagg acggataatt ttgttgtcct gacgttgttc aatgatgtgc gccgcccagc
    31021 cggtgacacg ggcgatgaca aacagtggtg tgaacatctc ggtgggaacg cccatcatgt
    31081 tgtaggaaac agcagagaac cagtcgagat tggggaacat ctttttgctc tcccacatca
    31141 ccgtttccag gcgattggcg atgttgtaca tcttcaacga gccgccttcc tgcgagagct
    31201 gcttcgccac acgtttgatc acctggtggc gcgggtcggc gatggtataa accggatgac
    31261 caaaaccaat gaccacttct ttgttttcca cccgcttgcg gatatcggct tcggcttcgt
    31321 ccggcgtttc gtagcgttgc tggatctcca gcgacacttc attcgctccg ccgtgtttcg
    31381 gcccgcgcag tgcgccaatc gcgccaataa tcgcggaata catatcagag ccagtgcccg
    31441 caatcacccg actggtaaag gtggaggcgt taaactcgtg ttcggcgtac agcactagcg
    31501 aaatatgcat cgccttttcc cagctttgcg atggcttttc gccgtgcagc agatgcagga
    31561 agtgaccacc gatggagtcg tcatcggttt ccggctggat gcgttcgccg ttgtgactgt
    31621 agtgatacca ataaaggaga atcgagctaa gtgatgccag cagtttgtcg gcaatatccc
    31681 gcgcgccaga gacagtgtgc ccctcttttt ctggcagtgt gcagccgagc gcggaaacgc
    31741 cggtgcgcat gacatccatc gggtacgacg ccgccggtaa ggcttccagc acggtacgca
    31801 cgttagccgg taaaccgcgc agggctttca gtttcgtttt gtaagcggcg agttcgtcac
    31861 gggtcggcag tttgccgtgg atcagcaggt gcgccacttc ttcaaattcg caatgttccg
    31921 ccagatcaag aatatcgtag ccacggtaat gcaggtcatt gccactttta cccacggtgc
    31981 agagcgccgt attgcccgcc ggaacgccag aaagtgccac agattttttc ggtttaatga
    32041 catgggtact gttttgcagg atcgttgtgt cgctcatgtt gtcctcgtca tttgtagggt
    32101 cgagatattt ttcgttgtgt tttatttcac ctggctacgg gcaaacaggt cgtcgagctt
    32161 cgcttcgtac tggtagtagt tgatgctttc gtacagctcg ttgcgggttt gcatggtatc
    32221 gatgacgctt ttctgcgtgc cttcctgacg caggacgttg tagacatgct cagcggcgcg
    32281 gttcatggcg cggaacgctg aaagcgggta cagcgccatt gcgacatggg cgctgcgtaa
    32341 ttcgtcgatg gtaaacagcg gcgtggcacc aaattcggta atgttggcga ggattggcac
    32401 ctgcaccgca tcggcaaact ggcggtacat ggcgagttcg gtaatcgcct ccgggaacaa
    32461 catctcggca cccgcttcaa cataggcctg cgcacgctcg atcgccgcat ccagcccctc
    32521 taccgccaga gcatcggtgc gcgccatgat cacaaaatca ggatcggttt tcgcatccac
    32581 cgccgcgcgg atccgatcca ccatctcttc tttcgagacg atcgctttat tcggacgatg
    32641 accgcagcgt ttcgcaccaa cctgatcttc aatatgcaat cctgccgcac cggctttaat
    32701 catcgatttc acggtgcgcg ccacgttaaa ggccgaagaa ccaaaaccga tatccgcatc
    32761 caccagcagc ggcagcgaac aaacgtcggt gatacggcga atatcggtca gcacgtcatc
    32821 aagagtagaa ataccgagat cgggcagccc cagcgaacct gccgccacgc caccgccaga
    32881 aagataaatt gcctgatatc cggcacgctg cgccaacagc gcatgattag cgttgatggt
    32941 gccaacaatc tgcaatggat tttctttagt cagtgcagcg cgaaacgctt tacctggaga
    33001 gtgtagagac atagcccatc ctttgttatc aacttgttat ttgcgttgat aaagacaaag
    33061 caaggggtgt gccacaattg ccttaataaa attaaatgtt taatttcaat ttgttagaga
    33121 taaagtcatg aactaatccg caaatatgcg tttcagttaa cgtttcaggc aatgtttcac
    33181 cgcgtttcat tgcaacaatt atgaaacaag actaaaccca atattcggtt tcttaacttt
    33241 gcggtgcgct atggcacatc caccacggct taatgacgac aaaccggtta tctggacggt
    33301 atctgtaacg cgcctgttcg agctgtttcg cgatatcagc ctcgagtttg atcacctggc
    33361 gaacattacc cctatccagc ttggctttga aaaagcggtg acctacatcc acaagaaact
    33421 ggcaaacgaa cgctgtgacg ccatcatcgc ggctggatct aacggcgcgt acctgaaaag
    33481 ccgcctgtca gtgccagtta ttttgattaa accgagcggc tacgatgtgt tacaggcact
    33541 ggcaaaagcc ggaaaactca cctcttctat cggcgttgtc acttatcagg aaactattcc
    33601 ggcactggtg gcgtttcaaa aaacctttaa tttgcgcctc gatcaacgta gctacattac
    33661 cgaagaagac gcacgcgggc agattaacga gctaaaagct aacggcaccg aagcggtggt
    33721 cggcgcgggg ctgattaccg atctggcaga agaagccgga atgaccggaa tttttatcta
    33781 ttccgccgcc accgtgcgcc aggcgttcag cgatgcgctg gatatgacgc gcatgtcgtt
    33841 acgccataac actcacgatg ccactcgcaa cgccctgcgt actcgttacg tgctgggcga
    33901 tatgctcggt caatcaccac agatggaaca ggtgcggcag actattttgc tgtatgcccg
    33961 ctccagtgcg gcggtgttga ttgaggggga aacggggacg ggcaaagaac tggcagccca
    34021 ggcaattcat cgggaatact ttgcccgcca cgatgcgcga cagggcaaaa agtcgcatcc
    34081 gtttgttgcc gtcaactgcg gggcgattgc cgaatcgctg ctggaagcgg agctgtttgg
    34141 ctatgaggaa ggggcgttta ccggctcgcg acgcggaggt cgcgccgggc tgtttgaaat
    34201 cgcccacggt ggcacgctgt ttttggatga gattggcgaa atgccgctac ctttgcagac
    34261 ccggctgctg cgggtgctgg aagaaaaaga ggttacccgc gtcggcgggc atcagcctgt
    34321 tccggtggat gtgcgggtca ttagcgccac tcactgcaag ctggaagaag atatgcggca
    34381 agggcagttt cgccgtgacc tgttttatcg gctgagcatt ttacgcctgc aactgccacc
    34441 actgcgtgag cgggtggcgg atattctgcc gctggcggaa agctttttga aagtgtctct
    34501 ggcggcgctc tccgccccgt tttctgccgc attacgcctg ggattacagg caagcgaaac
    34561 cgtgctggtg cactacgact ggccgggcaa tattcgtgaa ctgcgcaata tgatggagcg
    34621 actggcgcta tttttaagtg tggaaccgac gccggattta acgccgcaat ttttgcagct
    34681 gctactgccg gaactggcgc gcgagtcggc gaaaactccc gctccacgct tactgacacc
    34741 acaacaggca ctggagaaat ttaatggcga taaaacagca gcggcgaatt atttaggcat
    34801 cagccggacg acgttctggc ggcggctgaa aagctgaact gccgaatgca gcgcgaacgc
    34861 tttatccggc ataaatttca atagatgatc accgataaac ttacgcatcg ggcatgttta
    34921 catagggttc agaattactt cttcttataa atatttgccg tgccgtggat cttattgtca
    34981 gtttgaccgg aggtcagtac caacacatca gccccttttt catccgcttt tttgatcaaa
    35041 tcttctttcg cgtctgcagt cgacatttca ttgctagttg aaatatcacc tattttttca
    35101 tactgcgatt caactttttc aaattccgct ttggtcatca attcagcggc atagacattg
    35161 gtaacggcta acgctaacgc accgactaac attttagaga ttattttcat attacggctc
    35221 cagagttgtt atcacactat catccaccca cattattggg ttgattaata attagttcag
    35281 tcagggcata aacgccagta accggaaata agacactttg tgatctctct cgcacccgat
    35341 tatttttggt agaaatatga tgaagttact tcacttattt atggatgaaa tcactatgga
    35401 tcctttgcac gccgtttacc tgaccgtagg actgttcgtg attacttttt ttaatccggg
    35461 agccaatctc tttgtggtag tacaaaccag cctggcttcc ggtcgacgcg caggggtgct
    35521 gaccgggctg ggcgtggcgc tgggcgatgc attttattcc gggttgggtt tgtttggtct
    35581 tgcaacgcta attacgcagt gtgaggagat tttttcgctt atcagaatcg tcggcggcgc
    35641 ttatctctta tggtttgcgt ggtgcagcat gcgccgccag tcaacaccgc aaatgagcac
    35701 actacaacaa ccgattagcg ccccctggta tgtctttttt cgccgcggat taattaccga
    35761 tctctctaac ccgcaaaccg ttttattttt tatcagtatt ttctcagtaa cattaaatgc
    35821 cgaaacacca acatgggcac gtttaatggc ctgggcgggg attgtgctcg catcaattat
    35881 ctggcgagtt tttcttagtc aggcgttttc tttgcccgct gtgcgtcgtg cttatgggcg
    35941 tatgcaacgc gttgccagtc gggttattgg tgcaattatt ggtgtattcg cgctacgcct
    36001 gatttacgaa ggggtgacgc agcggtgagc agataattta ctctctacgt aatcaacctg
    36061 atttgccgaa tcccgaccat cggcgatgat tgcccccgta atgcgtggtc cttatttccg
    36121 ataagtttac ttgagtcttt agcttcccat ttcagcccac tctcccctgt gtgggctttt
    36181 ttcattggtc tgacccgtcc tgaatagcca aacaatggca atgttccact ttgcagggat
    36241 gataataagg ggatctgatt cagaagagtt ttgaaaagtt ggaccgccat cgaggcctcg
    36301 aaccccgtac catacaacaa tcctggttgt cgtctgctct tcctgatgag ctaatggcgg
    36361 tttttgatat gagccttaca gcatattttc tgtcaggctt gagtgcgatg ctaaggtaca
    36421 gaaaagaccc cacacaaaca tgatggaatt atgatttttt tcatatgaat gtcaacccac
    36481 acattcacaa aacatctgca tgagatcatt ttttatacac cgcatagctg cgaaaccact
    36541 gctggtgctt ttttaagcga agcgtcgccg atcacaacaa aatatcggcg aaaaaggcgg
    36601 tggaatgcca tttggcatac ggatatacag atgaaatgat tacctgcttt gcgattcact
    36661 ctctggtgga ataacaactt gcaggtggaa ccctttctga actaaagagt gtagcggatt
    36721 ttaacgtgct tatttacagg ataatatctg tcacctaatt cctgcactct attcgatatc
    36781 cattcattta atcgtgcgtc atctctgtat gtttttacgt ccagaacaga gagcaataac
    36841 atcaccagtg attcaggaaa aacagggtgg atatttttta agtgtaataa taatccatgc
    36901 gcaagcgcct gcttaatggg cgcgtctgtc tccggaatag acgacatttc gcgaagccat
    36961 ttttccacac gcatacactc tttcccttta gggaaaattc tcagcaaagg catatggtgt
    37021 tcaagaagaa tacatttgca atctgttcgc actgaatcga taaactgcac taatttctta
    37081 agcccggtaa agcgtccagc ctgcacattt gctttaatat caagaaacgc atcatcctgg
    37141 tcatataccc aaactgtccc tttttgattt tcaagtatgc gaaaaacaaa cttaaccggg
    37201 tgatcagggt aatccatcca gtcataaaat ccagggaaat cattaaactg cacatttatg
    37261 gtgagtgcgt tatcgtctga ccactcacag tctcgtacgg cagccatgag atgaatgagt
    37321 ttgtcgtcat cgactaaatc atcacgacaa tcataaggat aatcataata ttgtttaatc
    37381 aaaaccttga atatatcgac acaggaaacc agggagcttt tgatatcctc tggtaactga
    37441 ttgtttttaa ccgtattaag tatgctttgt tgcgtatatg gcatcaaatt attgtgcgga
    37501 gcttttaatg atatcatcgc gatctctctt aaataaaaat aaacgtcgca gataataaag
    37561 ctctcattaa accaggcact atcagaaatc gctcataact aacgtgtgaa gtattgtgta
    37621 ctggagggag ttaatttaac aatttgaatt tacgttatag gttttactgc tactttgata
    37681 ttatttcgct ctccttaact aaaaagttaa gtggagagcg aagttaatat gttaaaccac
    37741 agcgtattta ttaatttttt cagtctgtta gtgtgcgatt atcgataaca aaacgatatt
    37801 tcacatcacc acgcagcatt cgctcatagg cttcattaat ttgatcggcc cgaatcatct
    37861 ctatatcagc cacgatgcca tgttcggcgc aaaaatcgag catctcctga gtttctggaa
    37921 tgccgccaat catagaaccg gctatcgcac ggcgtttcat gatcaggttg aaaacttccg
    37981 gcgatttatg cggtgtcgca ggcgcaccaa ccagcgtcat ggtgccatca cgcttcagca
    38041 aggtggtaaa atcgtcgaga ttatgtggcg cagctactgt attcaaaatg aaatcgaaac
    38101 tcttcacatg agccgccatc tcatcggcat tgcgtgagtt aacaacttca tcggccccca
    38161 gggcttttgc cgcttcgcgt tttgcctcag aagtggtaaa tgccaccaca tgtgccccca
    38221 tcgcgtgggc cagcttaatc cccatatgtc ccaggccgcc gatgccgacc acgcccactt
    38281 ttttacccgg cccggcctgc cagtgacgta gcggcgaata cgtggtgatc cctgcacaca
    38341 acaaaggagc caccgccgcc agctgctctt gcgggtgacg aatacgcaga acatatcgct
    38401 catgaacgac gatctgttgt gagtagccgc ccagagtatg gcccggttcg tccggtgtcg
    38461 gcgagttata ggtgccggtc atgtgatcac agtagttttc cagcccgtct tcacactctt
    38521 cgcaatgttt acaactgtcg acaatgcagc cgacaccgac cagatcgccc ggcgcatgtt
    38581 tttctacctg atcaccaacg gctaccacac gccccacaat ttcatgaccc ggtacgcagg
    38641 ggtaaaccgt ccccgcccac tcggaacgga cctggtggag atcggaatgg cagacgccac
    38701 agtaagcgat ttcgattttg acatcattcg gtcccggttc acgccgtgtg atatccatcg
    38761 gttcaagtgg ttgtttagcg gaatatgcac caacagcttt gatcttcatt gtgtttactc
    38821 ctgattagct atgtgtattt ggcgatgcct gatatgtcat cgcggcaaaa cgcgtccatt
    38881 gaatatagcc aatatttaaa ccacccgatc cagccccaac atgccgcgaa cctgaatgaa
    38941 acagagcagc aacgttcccc ataaggccat cgtcaggtag gggcttatac cgagaatatt
    39001 gaagccgctt tcaataattt gcagaagaaa cagcgccagc accataccaa ttatccgccc
    39061 gctgccgccg tccggattaa ccccgccgag tactgccgcg aggattgaca ccagcaagta
    39121 cgattcgcca taagaggcct tagccgagtt gagtttcgac atcatcaaaa atgccgctac
    39181 cgcacaaagc aaggcggaaa tgacataaac ccaaatcagc acccgacgag tgttaatgcc
    39241 ggagtaaagt gttgctcgtt cgttagaccc aatcaggtaa atggctttac cgagcggcgt
    39301 tttctccagc aatatccaca gccccagtgc cactgcggca aacaaccaca tcggtagcgg
    39361 aataccgaac cattgcgcat gattcaacca cagcacccat gagggataat tggcaatggc
    39421 gctaccacct gtcaccagaa tatttatccc tttgagcaac gtcatcatcc caagcgttgc
    39481 aagaattggc gacactcgaa taccagcaat caatacgccg ttacataaac cgattatcac
    39541 cgccgcgcca gcgcccgcca gtaacgtagc gacaaccgta gcaatacccg gtggatactg
    39601 agtcgccacc cacgccatca ccagtgaaca ggcgttagca gtggctataa ttgacaagtt
    39661 aatgccaccg caaagcatgg tcaccgccat agccaacgcc agaatgccca gcacgggcat
    39721 ttgcgaagcg acggactgga aattacttat cgaccagaaa atctccggca tagtaatgct
    39781 gaacgcggca acagttagca ccagcaggcc gatcaagtaa aactcaacgt tattacgcca
    39841 cgattttttc atagcagact ccggttctga tgctgactcc acgccgtggc actaatgctg
    39901 gcaacgatga tgatgccggt gatcaatgtt tgccagtaag acgagactcc caataaattt
    39961 agcccgtttt gcatcactgc caacaacacc acgccgagca aagtaccggt taacgtgccg
    40021 cgcccgccga gcaaactagt gccgccaagc accaccgcag ccagtactgt cagctcataa
    40081 cccagaagag aatcgggggc gacagtcatc accgtccacg actgcactac acccgcagcg
    40141 ccagacatca atcccatata gccgtagacg aaaagttgca gtttcagcac gctaaagccg
    40201 atgcgactgg ctgattcgcg gttcccgcca agggcataaa ttttgcgccc tacactggtg
    40261 aaattcatga taaacgctgt cagcagcacc accgtaatca tggcgatcag cggcagacca
    40321 aggccatagt catagccatc agcatcggtg tacttgaaca acataacccc ctgctcaaac
    40381 cacggcggaa agtcgtacag ccacacacct ttactcaacc ataacagcag gccatagaaa
    40441 atattcagcg tcgagatagt gatgatgata gaaggcaccc gtaggcgatt aaccagtaag
    40501 gcgttaatta agccaagtaa aatgccgatg cagcccgcca gtgcaaacgc gacagcgaag
    40561 ttgccaccaa ttttttgcag caacagcacc atgccgtatt gcgcaatgat ggtcattgct
    40621 ggaaacgaaa tatcaattcc accggaaatc aacaccacaa acaaaccaca ggcgagaata
    40681 gtcaacatgg catagttatt ggcgagatcg tacaaattac cgaatgtcag aaactcgtcg
    40741 ctgcgccagg caaggccaac aaacagcacc acaatcagta atgccagcca gaattcatgg
    40801 cgttttttca gttcagccat ttaccacctc cgcaatcctg gcctgagaac tgctgtcagg
    40861 caggaagcta tgggtgatct gacctttttg cattaccaaa atccggtggc tttgatacca
    40921 cgcttcttca atttcatcgc agatcatcaa taccgcaatg ccatgagccg ccagatcgct
    40981 gataatgccg tatattcctg ctttgttggc gatatcaaca ccgactgtcg ggctatcgag
    41041 aatcaacagt cgtggcccaa tcgccagcca tttggcgatc gatactcgct gcgcgttacc
    41101 accagaaagc gtattcaccg gaagatgagg atcagagact ttgatggtca atgccttgat
    41161 tagcctgtcc accaggtcac gaactttagt ttcactcaga aagccgaagc gatcttttac
    41221 cttgtgaaaa acagtgctaa tgatgttgtc ttcaatagat tgcgccatca ccaaaccgcg
    41281 cgacatgcga tcttccgaaa cataaccaat accgtggcgg atagcgtcct ggttactatg
    41341 cagtgtaacc ggctggccgt tgatgaggat ctcgccagca tccggtcggg tcataccaaa
    41401 cagactcaga cataattccg tgcgccccgc accaagcagg ccaacaatcg ataccacttc
    41461 gcccgcttcc acccgcaagt taatatttag atattcccca tgacgactta aattacgcac
    41521 ctcaagcacc ggggtgctgg attttccctg ccacaactct cgcacctgat attcgaactc
    41581 ctggccggtc atcaggaaac cgagttgttt tgtggtcatc tctgcagccg gaaaagtccc
    41641 gaccagctca ccatctttca gcactgaaat acggtcggaa acttccatca cttcttccag
    41701 acgatgactg acgaagacca cgcagatccc gcgttcccgc aattgatgca ccacctgtaa
    41761 taacccttgc acttcctgat gggtcagcga agcggttggt tcatccatca cgatcaaccg
    41821 tgcgtcctgc gccagtgcac ggcaaattgc taccagctga cagcgggcaa ttgaaagctc
    41881 agaaacgagc gtatccagtg gcaatgtgac gttaatactg gtcattgccg cctgcgccac
    41941 ctcacgcaga cgacggcggt taacaaacag accgtggtga tagtgattca cggcgatatt
    42001 ctcccagaca ctcaggttag gaaataaaga gaggtcctga taaatcacct gaatcccctg
    42061 cgccaccgaa gcggcgggtg tcagctttga ccagtttttc ccaccaagcg taatttccgc
    42121 tccctcatcc gggcgataaa caccggacat aattttaatc aacgtcgatt taccacagcc
    42181 gttttgcccg gcaaggcagt gcacttcacc cgacataaag tcgagagaaa catcgcgcag
    42241 cgctcgcgtg gcatgaaacg ttttgttgat atgacgaagg gaaaggaagg tttccataac
    42301 atcccctggc aataagaatc ggggctttcg ccccgaagaa atggattaat aaagtgagtc
    42361 gatattgtct ttgttgacca gtaatacctt atggaaacgg ataattcgct tatccatatc
    42421 tacgtcggcc ttacccagat tctgcatttc taatccaggt ttgatctctt cacccttcag
    42481 caacgtgctg gcgacggctg cgagtgcgta acctgcagag gccgaatcgt aagtaatccc
    42541 ttcggtgata tcaccacttt taatcagtga tgccgcctgt gaagggatca tcatgccata
    42601 gactgcgact ttatttttcg cccgtttctc tttcaccgca cgtcccgcgc caatcggacc
    42661 gtttgaacca aaggagacaa ccgctttcaa gtcaggatag gttttcatca ggtccagtgt
    42721 ggtacgacgt gagtcatcca cactctcggc aaccggcatg cggcgggtaa cttcatgcat
    42781 atccgggtaa tgctctttct ggtatttcac cagcaagtca gcccataagt tatgctgcgg
    42841 cacggtcaaa ctacccacgt aaatcacata gccgcccttg ccccccatgc gtttcgccat
    42901 atgctcaaca tattcagcgg caaatttttc gttatcaatg atttcgatat cccagttagc
    42961 acttggctga ccgggggatt cgttggtcag aaccacaatt ccggcatctc gcgctttttt
    43021 gaataccggt tccagcacgt tggcatcgtt tggcacgata gtaattgcat taaccttacg
    43081 ggcgattaaa tcctcaataa ttttaacttg ttgcggagca tcagtacttg aaggccccac
    43141 ctgtgacgca ttaacaccaa aggcgttacc cgcctcaacc acaccttcgc ccatgcgatt
    43201 aaaccacggc ataccatcga ctttagagat attcaccacg actttttccg ctgcctggag
    43261 cggcgcagaa attagcgcag cgcctaataa cagcgaagac accatattga taacaaaacg
    43321 tttattcatc atttcagcct cattgcaggg tagtcaggaa gtgttcgccc aaattagcgc
    43381 gctggagaga ataagaattt tcgaatattt ttatgcagaa taaacagccc tatttcaggc
    43441 agctattaaa aatatggtta taaatatgga caaaacgggc agcaacaata agtgtgacat
    43501 tcatcacgta aaaaatagca aaataaacaa aattagcgat attttatgtg tgtgagttta
    43561 tttcgcgtag ataaatagtt atctacacaa cgtcaataag ggcatattct tcaaatataa
    43621 cgtccatttc tggtgaatag cttccttctg cctggaaaaa acgacggtgt tcattacgcc
    43681 aatattcaag actgaggtca ccttcacctt ccttacgggc aagctccacg gtgacatcag
    43741 aaaagcgcag taaatgcagg ccaatcactc tgataacaca tacaggctca ccacggctat
    43801 tttctacaat cttatactca ccaatcatcg gaaatgcgtt gtcttcaata catcccgcca
    43861 gcgaaccaca ggaagctgtt ttaacaccag tcgcaatcaa cgttgcgagg tgatcggcca
    43921 acgctgctga atcgccaaaa gtacaaaagg aagcatgggg atatttttgt gagaattttt
    43981 cttttatggt catggcaata tttttgcgat tacggaaaaa caaatcacat cttaattact
    44041 gttggtaacc atcagcaaga gtatcattgg tttttcgata ctgaatcttt tttatccgaa
    44101 gcaggtcaca tttatggcct gataaatacc tttgttaaaa ctcacttttg tgtatttctc
    44161 tcaaataatc ccctggaaat tatgccgcga gattataaag ttcaggatca ttcattagca
    44221 aaccattccc gccccactcc ctggggctta aatctcttac aattcaccgt tgcaggcgtt
    44281 tcaaatgcgc atcaacctgc cacactcccc cacaccagtt gccctttatg gaacgttgcg
    44341 gttctcggcg agatacgcgc caccgcctcg gcggaacagg aggcgtccac cagcacaaag
    44401 ctggcatcat cctgcgcttt tggccataca cgctcgcctt tttcattcag cggcaataca
    44461 tcgccagtgg ctagaaacag cgaacgggag aggttctgtt cgttaggacg aatatagagc
    44521 tgcgcgtaga gattggcttt ttccagcatg tcgcccagac cataaggcga ccagtggtcg
    44581 ataacgctgt cagtgccagt catcactttt acgcctttgt cgtgcaactg tttgagcggc
    44641 atatgcagcg tgccaatcgg caccgtcgag gcgatagaaa tttgctgcgc cgccatccga
    44701 ttcgccagcg catcgacctg ttgttcgttg agcgtagcca gcgcaaaggc gtggctgatg
    44761 gtcagcttac ctttcagttg cggcgttttc tctaccgttt caaccatata attgatggct
    44821 gccacgcccg acggagtggt ttcgtgcagg tgaatatcga cacctttgtc gtagtccagc
    44881 gcaatctgaa acatggtgtc gagggatttt tccatcgcgc catcgacact ggtcgggtcc
    44941 agcccgccga cgtaatgcgc acccgcctgc atcgcttcgc gcattaaggg ttccgatttc
    45001 gacagcagca aaccgtgctg cgggaaggcg acgatttcac actcgaagcc cgcctgacgt
    45061 cgcgccagca ccgcctgcaa attctgcaga tttttcaggc cggaaaccgg ttcgatattg
    45121 cagtggctgc gggcaatggt ggtgcctttc gactgcaata gatcgatcag tttttctgcc
    45181 cgttcctgcg tgtacggttg cagttccggc agcattttct gctcaagctt gatcatgtcc
    45241 tggatagtgg tgcctgccgg acgattgagc gagcgccacg gcccgccgta gaaggttttg
    45301 tcgagatgaa tatgcatgtc gcgagtggtg ggtagcatca acttaccgcc agcgtcatag
    45361 tgcggcagcg tggcgtccgg atgctgcttg ttctcgcgca gggcgacaat tttgccgtcc
    45421 tgaatctcca cggtctggcg cgccgtgcgg gtctgcaccg ccacaccgtt ttcgtagtca
    45481 aaaccggttt ccagcagcac gttatcgaga tagtaatgcg ggtcagtgat tttcatggtg
    45541 ttgttcgctt cgcagtttag ggtgccagct accgccgcat gggcgagcgg cacagaggta
    45601 ccaaacagcg cggcggcggt gaccatctta ccgctctggc tcagaaattc acggcggcta
    45661 ttgctttctt tcatcctacg tccttacgtt ttaataatat gggtgcccgt ttcaccgcgc
    45721 agcgccgcag gcaggcattc cggcgtggtg ataatcactt ctttgccgcc ctgctccagg
    45781 aacgtcaggc tggcgatgat ttttggcaac atgctgccgg gcggaaaatg cccttcctgc
    45841 atatagcgag tcatggtggc aatatccacc cgatccagcg cctgctgctg cggtttacca
    45901 aagtggatac agactttttc gacgccggtg gtgatcacca gaatgtcggc gtgaatttca
    45961 cgggccagca gcgcggtaga gagatctttg tcaataaccg cgtccacgct ttggtaatcc
    46021 cccgcttcag tacgcactac cggaattcca ccgccgcccg cgccaattac gacaaaacct
    46081 tgttggatca gcgccttaat ggcgggtgct tcgacaatac gtttcggttc cggcgaggcg
    46141 accacgcggc gatagccccg tccggcatct tcaacaaaac accagtcagg gtttgccttt
    46201 tgtagtttgt cacgctggct ttcactgaag aatgcaccaa tgggcttggt gggatgggca
    46261 aaacctggat cgtttttatc cacttccacc tgagttacca cggtgacggc ttttttctcg
    46321 ccgtgacgcg ccagccggtt attcagcgcc tgttggatca gatagccgat gccgccctgc
    46381 gtatctgcca cacagttcgc cagcggcgtt aagggcagcc cttcgcgctc gtgggcaatc
    46441 tccgcacggc gtaaatccag cccgacctgc ggcccgttac cgtgggtcag cacaatgtcg
    46501 taatcggaag ccagcatttc caacaccgta tcggccacgg ctttcaccgc ctccgcctga
    46561 tgctcaatcg actggctggc gttatctttg ataatgctgt tgccaccaat ggcaacgacc
    46621 acaagctctt tcatgttgtt atgtccgcta cagagggtga ttaggttatg accaacgcca
    46681 gactgcctga tgcgctacgc ttatcaggcc tacaattctc gggccatccg taggccggat
    46741 aaggcgttca cgccgcatcc ggcatgaaca tgtaggtcag acgccgtaac gttcgcacca
    46801 gccaagaatg gctttctcga agcaggcaaa aggtggatgc acaatgcccg cgccaatcat
    46861 gccgacgccc gcatctttat gggcgatggc agtgttgatc accggcagaa tgccgctgct
    46921 acccacgcgg gtgatgtcga ttgccgacgg cacgcccata aaaccgagca gcggaatggt
    46981 gacgttgggg ttttcaccga gagtgatttc acgcatctgg cgagagaaat cgatagcctc
    47041 ttccaccgtg ccgcccacca gcgcaacgat tgcaggcgcg gtcgccatag caaatccgcc
    47101 aataccgtag gtttcagtga tggcgctgtc gccgatatcc agccccgaat cttccggctt
    47161 ataaccggca aacatcgggc cgatgacctg ctgcgccgga ccggtaaacc attgccccgg
    47221 taatccgctg acccgcaggc cgaactcgac gccgttacgc gccatggtgg tgaccacggt
    47281 gctgtattcg atgccgtgcg ccgcatccat cgccgcttta cacatcgcca tccacgtcgg
    47341 gccggagaag tagtcgctgc tagcgacaaa ctcaaacact tcgcgctgtt tctccacgga
    47401 ataacctgcc tgaataattc ccggcgtcag cgcctgaatc agcaacgtcg tcccggcgtt
    47461 attgcggtta tggcactcat cgcccatatg cagcgcctgc gccagcatta agcgcagatc
    47521 aatctcgccg ataattttca tcgcgtcgcg cagcattggc ccctgcacat cgcgcatcca
    47581 gttgaggcgg tcgatcacgc tctgatcgtt ggctcccata cgcaaaatct tcgccatctg
    47641 ctcgctcata ttggtgtaag cgatgttgcc gtaggttttg ttttttacga tgtgcataaa
    47701 catcgaggca gaggtaacac ccgccatcga tcccacacag tcgtgttcgt ggcacggcga
    47761 gaaggtgatc tccccggagg ccgccagttc agccgcctcg tcgagatctt tcgccagccc
    47821 ttcgaacacc aacgccccgg tgaccgcgcc tttcatcgcg ccgcacattt tttcccaggt
    47881 gatcggcggc ccggcgtgaa gaatggtttt cggcgtcatg cccggcacca cgttaatcgc
    47941 ctgatcaaaa ccaatcagca ccggatgcga ctggataatg cgctccagcg cctgctggtt
    48001 agcggcggcg attttgtctg ccagcggcga atcggcaatg ttatccagcg cctgcaccac
    48061 ctgcatattg ccctgccccg gcggcgtcca gtcgagttga gtcacttcta catgctgctt
    48121 tttcaggtca tcgctaaaca tggcgatgcc gacgttaata acgttcaatg gttggctaaa
    48181 cagtgactgg ctcatcaggc ttcctcccct ttgcagataa attcacgcgc cagcaatccg
    48241 gtattggtgc tgctgctcgc cagaatcact ccggcatcaa gcagcatctg gctttgttgt
    48301 tctaacgatg gcgtatcaag atcggtaccc agcacatagg cgagaatgat caactcgcgt
    48361 ccttcggcag cggcgatcgc tttcgcttct ttgatcgcct cgatggtgga gccgaccgga
    48421 tcttcatgcg atccaaatcc gagcacaaaa tccatcacga tcaccgccac ttctggatcg
    48481 cgcgcctctt cgatcaagcg actgatgcgg ttggtggggt caatcattgg atgcggcttg
    48541 ccgttcgtga agtcgtcatc gccaaagtcg aggaaggtgt gtttgatgct gcggttgata
    48601 tcttgcaggc ggaattccgg atcgggctga atgttgctgt agacatcgcc atgtttttcc
    48661 atcaccgcga acatggtttc gtcgcacagc gtgccgccgc agaacaggcc acgaatgtat
    48721 ttctgctgcg gttgcagacg cgcacgcaca tcagcaatca acggctggtt aagcgtatgc
    48781 aggtcgagat tttcctgttt cacgccggag agcatcaccg cttttagcgc cgcctctttg
    48841 ctgccgcggg caaactgtag cccctgctca tccaccggcg tttcaacgcg accgaggaag
    48901 cagaccacca ccggcttgcg gcaggcacgt gcgcgttcca gcactttgcg ggccaccgca
    48961 ggcgcgggag gttttgaaat cagtgcgata atttcagttt gcggatcgtt ttccagcatc
    49021 ccgatggcgt cgagcatcat caggccgccg attttctcgc tcaggtcgcg cccgccggtg
    49081 ccaatcagtt gtgaaacacc gccaccaaat tcatgaatgc ggacgctcag ctcctggctg
    49141 ccggtgccgg atgcgccaac aataccgata ttgccgcgac gcacggcgtt accaaaacag
    49201 agcgccgcgc cgttaataat cgccgtgccg cagtccggcc ccatcatcag cagccctttt
    49261 tcgtgggcca actgcttgag cgccagttca tcttcaactg agacgttatc ggaaaacagc
    49321 atcacgttga gatcgttttg cagcgcctga cgcgcttcgc gagcggcaaa cagaccgttg
    49381 accgaaatca ccgccaggtt actttccggg atatgctttt tggcgctggc aatagtggcg
    49441 taacgcgcct cgtgcgagcc gctttgcgct ttggtgttga acagttcttc aatctccacc
    49501 agcaactgct cgttgtccgc acccgattta ccattgatga caatcatcag gtcgccgttt
    49561 ttcgcctgtt ccagctccgg cgtcagcagt cctaaatttt tcagtacacc tttgttcatt
    49621 tcggtcgcca tcgccacaaa tgcctgctcg acgccgtcga gtttatttgc acgcgtggag
    49681 atagacatca gcgagacaga atcaaaatag gtattcggtt taatgactat tttaactgac
    49741 ataattccct ccgtaggttt ggctcagcgc acaaccatcg gccatgccat acagcatgtc
    49801 gcagccggaa ctggagccaa tatgtttaat tttttctatt gcctgagttg cgttcccagg
    49861 gatgtcataa ataatgttgt gaataaaacg atgaatattt tcccggcaac gttgttgtaa
    49921 tgcggcttcc agcgttatag cacttaataa tgtggtgtta tttctgccgc gctgcatacc
    49981 gagataaaat tcctctttgt atttttccga cggatgcccg ggaataaata aaataagcgc
    50041 cagacctgtt aaataatcgt cggctgatgg cgtaaggcca atccctaacc caatcatact
    50101 ggcgaccgct gcggcgatat tttgtttttc gtttactgcc tgaataactg cccgtcgttt
    50161 aatatgtagt tctttattta acgcctgata aaacggatta tcgccgcgat aattaaacag
    50221 ggtttcattt tcccccaggt gctgatgaat aatgtcgtgc cactgtagcc aggcgataag
    50281 gtgaaaattc acagcggtca tttgcaaggt tggggacagc caacgccgac aacgactcat
    50341 ctctatatga agatgttgac caaccgtaat accttgatcg tcaaactgaa ccttatcacc
    50401 atgccggaac agatcatcaa agtgagtgag tgccaaccga caactgtttg gcgcattgtc
    50461 gtactcttca caaagcagcg tcagcaactg ctggcgagcc gggagtgcga tatttaccgc
    50521 tctggcaaag acctgttcaa cctgtccttg cccccgctgc gccagaaaat ccgcatccgc
    50581 agttagcgcc cacacacacc gtctttctgg tgtgttgata gcaagcctcc cggtttatgc
    50641 acctgcggca atcagtaact gcgcaatctc ttcaaagccc tgttcccgcg ccagttccag
    50701 tggcgttttg ccatatttat cggtcagatg cgggctggca ccgtgttcca gcaataactg
    50761 cacaatcgcc tgctgtttaa taccaccatc attaagcaca atcgcttcca gtagcggcgt
    50821 ccagcctaca tggttggtct ggttaacgtt aatctccgta tgcgccaaaa gctcttttac
    50881 aatacttaaa tggccttttt cacaggcagg cgtcaggccg acaccgccaa aacgggttac
    50941 gcaattaaga tcgggtttag ccggtaaaat aattcgtagt agcgttaaat catcgttcag
    51001 acagctaatt aaaaaaggat ttaaacaggt atgatcttgc ttattaatat ccgctccggc
    51061 atcaattaat gcctgaacgc aagcatattg ctgatataaa cttgccagcg taattgccgt
    51121 tttcccctga cgatcgcagg tattaatatc gacacccagc gcaaggcagg tttttacttt
    51181 atcgatatca ccctgttgtg cagccaataa atagtcggca ggtagatttt ttatactcat
    51241 tcacattccc ctggtttttg tggctgtttt cgatgaattg agaatagcaa cgataatctg
    51301 ataaaagaac ccgcttaaat ttaattcgct gtatttcgcc tttttgttga atgatgttca
    51361 acaatctttt cgcagtgtgc ggtagtgcaa tatttgtttc tttgacccga taccctttca
    51421 ctcaatcagg ttttatttct ctatagtctg cacatgtaag agtagctttc gtccatgtct
    51481 aaccagaggg gaacaagatg aacggactca cggcaacggg tgtcaccgtc ggtatttgtg
    51541 cagggctttg gcagttagtg tcttcacatg tcgggctctc tcagggatgg gaattattag
    51601 gaaccattgg cttcgtcgcc ttttgcagtt tttatgccgc aggcggcggt aaatcggggt
    51661 ttatcagaag tctggcggtt aattattccg ggatggtgtg ggcatttttc gccgcgctgg
    51721 ctgccggttg gctggcctct gtaagtggac tgtccgcgtt ctgggcgagc gtaattacta
    51781 ccgtaccctt ctcggcggta gtggtctggc aagggcgttt ctggttactg tcctttattc
    51841 ccggtggctt tctcggcatg acgctgtttt ttgccagcgg gatgaactgg acggtgacgt
    51901 tactgggttt tctggcgggt aactgcgtgg ggataatttc agaatatggt ggacaaaaat
    51961 taagcgaagc gacgactaaa cgcgacgggt attaaaagtt attcaaaaat gattatctat
    52021 ctttaaccga tacctctaca gtcatagctg gagaaattca tagtagataa tggcgagaat
    52081 ggtttaatga actcaatttt taccgaagag aatctgttgg catttactac cgctgcgcgt
    52141 tttggcagct tcagtaaagc cgcagaagag ttgggtttaa ccacttccgc cattagctac
    52201 accatcaagc gtatggagac ggggctggat gtggtgctgt tcactcgcag tacccgcagc
    52261 attgagttaa cggagtccgg gcgttatttt ttccgcaaag ctaccgacct gctgaatgat
    52321 ttttatgcca tcaaacgcag tatcgatact atttcgcagg gcatagaagc gcgtgtgcgc
    52381 atttgtatta atcagctgct ttatacgccc aaacacaccg cacgcttgtt acaggtgctg
    52441 aaaaagcagt ttcctacctg ccagattacc gtcaccaccg aagtgtataa cggcgtctgg
    52501 gatgcgatta ttaataatca ggccaatatc gccatcggcg cacccgatac actactggac
    52561 ggcggcggta ttgattacac tgaaattggc gcgattcgct gggcttttgc tatcgccccg
    52621 gatcacccgt tagcctttgt gccggaaccc atcgccgaaa gccaactgcg tctctaccct
    52681 aatattatgg tggaggacac cgcgcatacg attaataaaa aagtgggctg gttgctgcac
    52741 gggcaggagt caattctggt gccagatttc aacaccaaat gtcagtgcca gattttaggt
    52801 gaaggaattg gttttttgcc ggattacatg gtccgtgagg cgatgacgca atccctgctg
    52861 gttacccgac aaatccataa tccgcgccag gattcgcgca tgttactggc gactcagcat
    52921 tctgctacgg gtcaggtgac gcagtggatt aaaaaacaat ttgcgccaaa tggcatatta
    52981 acggggatct atcaggattt actgcatcgg gagaattaat cgctgccaac ctcccgatgc
    53041 gctgtgaata gcggtttaca tcaaccacct gctttcatta cccattcaga gataaattta
    53101 ttacccggta ctggcggaga gtacaaatag ccctgcaaaa agtgaacgcc tttaccgatc
    53161 attaaatccg cctgctcctg ggtttctacc ccttccgcca cgatactcag accaggctta
    53221 cgcgctagtt cgacaatatt gtccacaata tgaccggaga tttcgtcgac actcgccatt
    53281 tgcacaaatg acttatcgat cttaataaaa tcgaccggga acgcctgcaa gtaacgatag
    53341 gtcgcataac ccgtaccaaa gtcatccagc gcaaaggtaa tgttgtgctg atgaaggctg
    53401 tcaaatatcg ctctggcttc tggcgttacc ggaatagggt tacgttccgt tagctcgaga
    53461 accagcttga ttttatcgtt acctaatttc ttaaccaggt tcagacactc tttttcaaat
    53521 cccgctgcca aaaaacaacc cgccgagacg ttgatgccaa tatggaaatt gtccggcagc
    53581 aaatgtttta ccggcatcag aatatccgca gtctgtttca tcagttggcg ggtcattatg
    53641 acaataagac cggatgactc cgccagagga ataaactgat ccggtgggat aattcccgtt
    53701 tgtggatgtt cccagcggac aagtacctca cagcccgtca gtaccccagt ctgcgcgcag
    53761 aaaaccggtt ggatccacgg tttgaattcg tggttttcca acgccagact gatggcttca
    53821 ggcgtgacga tatggtgaca acggttatca gggatataaa aattattttt atttcccgtg
    53881 ccactgatga cttgcggatg atattgaaaa aagatatccc gccagaaggt aatatcactc
    53941 tggatgccta gtttttcata cagctgcttc ttttgatgtg aaattgtctt tgcactgcga
    54001 tttctgtact gtgagatttc tgtaaccgac attccctcca ttagcagctt gagtaaaagc
    54061 cgttcttttt cggacacgat accaggcaac gtcaaattca ccgttgcccc gaactcttcc
    54121 aaaaaaacac tcaaatcatg cgaagccatt gaacacacct tgattaaatc ttgtgcgcca
    54181 gctaaatctg cgagtcttca ctctgactga taaagctggc tgcaagaaaa tgacacagag
    54241 agccgaatta tctcccgttc ggctctctct ccttttactc attattcaga tcagaattgc
    54301 cacttaatgc caaccattgc agaggtgtca ttatatcccc tatcggcaac ctgaacgccc
    54361 acattccccc agacattcag gttggcattt agctgtcctt ccaccccggt ttttatctca
    54421 gcaatatttc gggctccatc ctgagtgacg gacacgccat ccatactggt gctgaaatcc
    54481 ttactgttat gcagccagtt cacttctaca aacggctgga actcgcggga tttaccgtca
    54541 tccattttat ggtggctctt cagccaggtt tttacgccaa gtcgggtttg aacattgcca
    54601 tcaccgttgc tatgaacgag ggttccgttg ctttcgcggt gcttatcggc tttgactccc
    54661 atccaggtaa cctgtgcttg cggctgaaca taccattcat tacgcgttcc ctggctgcca
    54721 ttaaattcag ctaatttgtg tttgtatcca gcttccagtg aagcggtaaa tccttttgat
    54781 ttataggatt cactttgtaa gtcatcccct ttcactgtgt tatcaaacca gctgtactgc
    54841 gcccaactgt cgagatacgc gccattacgc gactcgtcat cggcatacca ggtggcatag
    54901 aggccggtgc tatatccgtt cacactcgct tttgcacgat aaccggttcg cgaggaaatg
    54961 gttttgctgt cgctgttgcc atatcccgcc atgaccccaa catgccagct gtcgctgccg
    55021 ttttggctcc actgcgcgac atcgcctccc agttgcagaa catagcgatt gctttgggtt
    55081 ttcagctggc cgctgccatc acgccattta ttatgaccac cttcatggcg catccacata
    55141 gtggtttgtt tctgctcacc cgtcaccatg tcggtatagt acgtattacc cagacgctca
    55201 tgcagacgcg tggtgaacat tgtattcgct gctgcaaggt tcgcaatgta gctacccgcc
    55261 tccggtcgca ggtcattatc cacattcaga tccggacccg gcgtaggtgt cgggttaggg
    55321 ttcggctctg gattcgggtt tggctccgga ttcggcatcg ggtctggctc cggctgcagt
    55381 tcaggagaat cactgccgct ggtcagatac cagttaccac tatttgcccc ttgtccacgc
    55441 gcgagagtgt agtcatacgc ccccgcaacg atacgaccag cctgaacaaa ttcgccctca
    55501 gacttaccgt ctacatggat aagttcgata ccgttaaggg ttttcgcacc tgtaccgcca
    55561 gcgttattca ccgtaacggc agtcgtgccg ctagtatcgc cctcgacaac caatttatcg
    55621 gttactgagt catcgccatt cagtaccgta ttcattacta gttgaccgtt attaccgtgg
    55681 tagttcccct tcaccactaa cacattgcca gcttgctgtc ctgacattcc ggtcatcagc
    55741 gtaccgttgt tggtcagatt gccgcccagg gtgtaattca cagacgcagc cgctcttttt
    55801 tcagtgctat tattcaacgc cagggtaccg ttgttctcaa cgtttgcgct acccaatccc
    55861 tgcgcatgac ttaccaggac attagccgaa ttagcaatgt tcacattcgc cgtcaggttg
    55921 ctgttatcgc ccgcgatgtc cagcgtgccg ccctgaatat tcagctctcc gctccccgtt
    55981 aacgaaccgg ttgaagtgcc cccgttggta accgtcagac tgccgccatt taagctcagc
    56041 agtgaatcgg cgctgccatt gagcgtgccc acggtctggc tgtgaccatt catatccagt
    56101 tcagtctctg ccgccagacg cagttcatgg gtattaccca acacgttatc gttattcaac
    56161 aaaagcgtcc cactgcgcag atcggtaaca ccggtatagt cgttgtcttt gttagaaagc
    56221 gatacggtct gacccttctg gctgctgaat gccaggtcac cactgccggt aatctttgcg
    56281 ctgagatcgg cggcattctc gcttttaccg ttagagctca gcaccagagc atcgctgtcg
    56341 gtagcgtgta aatcaagctg ggtcaggccg taaccgatat acagaccgtc attgtttacc
    56401 ccactcgtca gacgatagtc gtaagtgccg acggccgctt tttgcccatt ctgctgaata
    56461 tcacgttggg cgctgctgga gaggatttgc ccatttttat cctgcaattg cagattgccc
    56521 gcatcgccca gaacttccgc accttgcgca tcaaccagct taatggcgct gttaccatca
    56581 tcgacttcag tgagcgataa cgcagagtca atatcacgag atactgagcg gacaacgtca
    56641 ctgtcagaaa cctgaatcac accctcgccg cgcaaatcca gcgttttact aacgttgacc
    56701 gtcccttctg tcatctgtgc acctgctgtc agggcaccaa aatcaacggt tccgccggag
    56761 aatcccaaag tgctaatgga gtgagcgccc ttacccactg ttacctggct tccctgaccc
    56821 gcagtgatcc cgcctctctg cagaaactca gcattctttt ccaggttaaa tgtcgtgttc
    56881 tggaacgcca gtgaaccctg gaaccagtct gtataagcaa actcatcgaa ggagaattcg
    56941 tgattcccca tgtttatttc aacaatacct ctaccttgaa gatcgttatc aaatacccaa
    57001 tctttattgg cattgatttg cagtttgcca tcaacatcca ggtccgcacg gccgatgttc
    57061 ttgtcttcag aaataaccgc gacagcacca gaagcgacat caatatatcc gctgaatgca
    57121 tagttattgc cggtcagtgc cactgtccca ctgtcgatac ttattgtacc attgccggaa
    57181 atcttattgg caaaggtcat gctatcgtca gccttcaggt gtaatttccc gcggttgttg
    57241 atctctgctg tacctatatt tttggtgatc cctaaagcgc cattttgctc aattttgact
    57301 ttaccactaa aggcagagtt atcaccggtt aagtaagtcg tgccattgag cgcattgata
    57361 tagcccaggc cctcgctgcc actgacaata tccgcggaaa tagtcgcgtt tttatccgtg
    57421 tggtttaagg tgattttctt atcggatgtc tttgcccaca caaactcaat tttttcggca
    57481 tcaataatcc ctgcagcctt cgcagcactt ccttcctgtg cacctaacgc taactcagag
    57541 ttggtgctta aactaatttt cggtgcggat attttacctc catctgatac gactaatgtt
    57601 gtatcagaac gttggtcgta ataacccagg ctaatatgtt cacgagcatt aacttcacca
    57661 ccgttattaa ccgacaggaa ctgattaccg ccatatccgc cgtaaacgcc cataaagaag
    57721 gtccctgcat ttacctgact accttcgccg cttacattcc cctgcgtata agtcagacga
    57781 ctggtgccct taacatgaat tttgccgcca ttggagacat taaaatagcc ttgatcatgt
    57841 ctcatgcctg agtttgactc atacaaatca tcagcatata actcgctgcc agcattggag
    57901 acattcagga accccgtacc tttcagcaaa aagttaagcg ggccattgat tgttaccttt
    57961 cctccggtaa tatccaaccc accaccagaa gcgagagtca gacgcggacg ggaagtatct
    58021 gtcgtattga ttaccagatt ggcattgtca gtcaccgtaa tacggtaatc gatgctctgg
    58081 ttctctttgc caaaatcaat agttttatcc tggtttacga tgacatcatc ggcgaatacc
    58141 agagatgaat ataaacttat cgcgctaatt aaaacagccc gggacttttt tccttttact
    58201 ctgctcccca gctcagagca gacgctccaa cagttacggg acctgttcca ctttagccga
    58261 tatattttat tcatcacaat ctccagcaat acatccaaat aaaaccaaga ttgcataaac
    58321 agcaacgcat taaatgccat tctggttact gttcatcgca tataaaaatt caaaaatttt
    58381 gcatagcatt ggcaaccaca tatctgagta tgtagttcag agggtaataa aatagcaagt
    58441 tattacttaa aacttatttt aagttaacac tcttaacttt atcattacat tcctcccagt
    58501 ctctaatacc tgcgcattat atgtatccag gaactcaaat caactcaaaa tataaacaaa
    58561 agcaagagga atacatttaa tatacagccg a
//
LOCUS       DAZFJF010000015        58537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFJF010000015 DAZFJF010000000
VERSION     DAZFJF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.015"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..464)
                     /gene="folK"
                     /locus_tag="ACHKAU_001073"
     CDS             complement(<1..464)
                     /gene="folK"
                     /locus_tag="ACHKAU_001073"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO5530185.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFD"
     gene            complement(461..1858)
                     /gene="pcnB"
                     /locus_tag="ACHKAU_001074"
     CDS             complement(461..1858)
                     /gene="pcnB"
                     /locus_tag="ACHKAU_001074"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO5530186.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPLPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1918..2814)
                     /gene="gluQRS"
                     /locus_tag="ACHKAU_001075"
     CDS             complement(1918..2814)
                     /gene="gluQRS"
                     /locus_tag="ACHKAU_001075"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO5530187.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2881..3336)
                     /gene="dksA"
                     /locus_tag="ACHKAU_001076"
     CDS             complement(2881..3336)
                     /gene="dksA"
                     /locus_tag="ACHKAU_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO5530188.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3514..4218)
                     /gene="sfsA"
                     /locus_tag="ACHKAU_001077"
     CDS             complement(3514..4218)
                     /gene="sfsA"
                     /locus_tag="ACHKAU_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO5530189.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4233..4763)
                     /gene="thpR"
                     /locus_tag="ACHKAU_001078"
     CDS             complement(4233..4763)
                     /gene="thpR"
                     /locus_tag="ACHKAU_001078"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO5530190.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4837..7266
                     /gene="hrpB"
                     /locus_tag="ACHKAU_001079"
     CDS             4837..7266
                     /gene="hrpB"
                     /locus_tag="ACHKAU_001079"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO5530191.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7398..9932
                     /gene="mrcB"
                     /locus_tag="ACHKAU_001080"
     CDS             7398..9932
                     /gene="mrcB"
                     /locus_tag="ACHKAU_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO5530192.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10152..12395
                     /gene="fhuA"
                     /locus_tag="ACHKAU_001081"
     CDS             10152..12395
                     /gene="fhuA"
                     /locus_tag="ACHKAU_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO5530193.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TVAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12446..13243
                     /gene="fhuC"
                     /locus_tag="ACHKAU_001082"
     CDS             12446..13243
                     /gene="fhuC"
                     /locus_tag="ACHKAU_001082"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO5530194.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13243..14133
                     /gene="fhuD"
                     /locus_tag="ACHKAU_001083"
     CDS             13243..14133
                     /gene="fhuD"
                     /locus_tag="ACHKAU_001083"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO5530195.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14130..16112
                     /gene="fhuB"
                     /locus_tag="ACHKAU_001084"
     CDS             14130..16112
                     /gene="fhuB"
                     /locus_tag="ACHKAU_001084"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO5530196.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16147..17427)
                     /gene="hemL"
                     /locus_tag="ACHKAU_001085"
     CDS             complement(16147..17427)
                     /gene="hemL"
                     /locus_tag="ACHKAU_001085"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO5530197.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17593..17640
                     /gene="yadX"
                     /locus_tag="ACHKAU_001086"
     CDS             17593..17640
                     /gene="yadX"
                     /locus_tag="ACHKAU_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO5530198.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17652..19073
                     /gene="clcA"
                     /locus_tag="ACHKAU_001087"
     CDS             17652..19073
                     /gene="clcA"
                     /locus_tag="ACHKAU_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO5530199.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19097..19162
                     /gene="yadW"
                     /locus_tag="ACHKAU_001088"
     CDS             19097..19162
                     /gene="yadW"
                     /locus_tag="ACHKAU_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO5530200.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19155..19499
                     /gene="erpA"
                     /locus_tag="ACHKAU_001089"
     CDS             19155..19499
                     /gene="erpA"
                     /locus_tag="ACHKAU_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO5530201.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19546..20169)
                     /gene="yadS"
                     /locus_tag="ACHKAU_001090"
     CDS             complement(19546..20169)
                     /gene="yadS"
                     /locus_tag="ACHKAU_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO5530202.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20207..21007)
                     /gene="btuF"
                     /locus_tag="ACHKAU_001091"
     CDS             complement(20207..21007)
                     /gene="btuF"
                     /locus_tag="ACHKAU_001091"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO5530203.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21000..21698)
                     /gene="mtnN"
                     /locus_tag="ACHKAU_001092"
     CDS             complement(21000..21698)
                     /gene="mtnN"
                     /locus_tag="ACHKAU_001092"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO5530204.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21782..23299
                     /gene="dgt"
                     /locus_tag="ACHKAU_001093"
     CDS             21782..23299
                     /gene="dgt"
                     /locus_tag="ACHKAU_001093"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO5530205.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23429..24853
                     /gene="degP"
                     /locus_tag="ACHKAU_001094"
     CDS             23429..24853
                     /gene="degP"
                     /locus_tag="ACHKAU_001094"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO5530206.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25010..26161
                     /gene="cdaR"
                     /locus_tag="ACHKAU_001095"
                     /pseudo
     CDS             25010..26161
                     /gene="cdaR"
                     /locus_tag="ACHKAU_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26250..26636)
                     /gene="yaeH"
                     /locus_tag="ACHKAU_001096"
     CDS             complement(26250..26636)
                     /gene="yaeH"
                     /locus_tag="ACHKAU_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO5530207.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26798..27610)
                     /gene="yaeI"
                     /locus_tag="ACHKAU_001097"
     CDS             complement(26798..27610)
                     /gene="yaeI"
                     /locus_tag="ACHKAU_001097"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO5530208.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27665..28489)
                     /gene="dapD"
                     /locus_tag="ACHKAU_001098"
     CDS             complement(27665..28489)
                     /gene="dapD"
                     /locus_tag="ACHKAU_001098"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO5530209.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28520..31192)
                     /gene="glnD"
                     /locus_tag="ACHKAU_001099"
     CDS             complement(28520..31192)
                     /gene="glnD"
                     /locus_tag="ACHKAU_001099"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO5530210.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31254..32048)
                     /gene="map"
                     /locus_tag="ACHKAU_001100"
     CDS             complement(31254..32048)
                     /gene="map"
                     /locus_tag="ACHKAU_001100"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO5530211.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32416..33141
                     /gene="rpsB"
                     /locus_tag="ACHKAU_001101"
     CDS             32416..33141
                     /gene="rpsB"
                     /locus_tag="ACHKAU_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO5530212.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33399..34250
                     /gene="tsf"
                     /locus_tag="ACHKAU_001102"
     CDS             33399..34250
                     /gene="tsf"
                     /locus_tag="ACHKAU_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO5530213.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34397..35122
                     /gene="pyrH"
                     /locus_tag="ACHKAU_001103"
     CDS             34397..35122
                     /gene="pyrH"
                     /locus_tag="ACHKAU_001103"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO5530214.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35414..35971
                     /gene="frr"
                     /locus_tag="ACHKAU_001104"
     CDS             35414..35971
                     /gene="frr"
                     /locus_tag="ACHKAU_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO5530215.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36063..37259
                     /gene="ispC"
                     /locus_tag="ACHKAU_001105"
     CDS             36063..37259
                     /gene="ispC"
                     /locus_tag="ACHKAU_001105"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO5530216.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37448..38206
                     /gene="ispU"
                     /locus_tag="ACHKAU_001106"
                     /gene_synonym="uppS"
     CDS             37448..38206
                     /gene="ispU"
                     /locus_tag="ACHKAU_001106"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO5530217.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38219..39076
                     /gene="cdsA"
                     /locus_tag="ACHKAU_001107"
     CDS             38219..39076
                     /gene="cdsA"
                     /locus_tag="ACHKAU_001107"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5530218.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39088..40440
                     /gene="rseP"
                     /locus_tag="ACHKAU_001108"
     CDS             39088..40440
                     /gene="rseP"
                     /locus_tag="ACHKAU_001108"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO5530219.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40470..42902
                     /gene="bamA"
                     /locus_tag="ACHKAU_001109"
     CDS             40470..42902
                     /gene="bamA"
                     /locus_tag="ACHKAU_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO5530220.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43024..43509
                     /gene="skp"
                     /locus_tag="ACHKAU_001110"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43024..43509
                     /gene="skp"
                     /locus_tag="ACHKAU_001110"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO5530221.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43513..44538
                     /gene="lpxD"
                     /locus_tag="ACHKAU_001111"
     CDS             43513..44538
                     /gene="lpxD"
                     /locus_tag="ACHKAU_001111"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO5530222.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44643..45098
                     /gene="fabZ"
                     /locus_tag="ACHKAU_001112"
     CDS             44643..45098
                     /gene="fabZ"
                     /locus_tag="ACHKAU_001112"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO5530223.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45102..45890
                     /gene="lpxA"
                     /locus_tag="ACHKAU_001113"
     CDS             45102..45890
                     /gene="lpxA"
                     /locus_tag="ACHKAU_001113"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO5530224.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45890..47038
                     /gene="lpxB"
                     /locus_tag="ACHKAU_001114"
     CDS             45890..47038
                     /gene="lpxB"
                     /locus_tag="ACHKAU_001114"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO5530225.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47035..47631
                     /gene="rnhB"
                     /locus_tag="ACHKAU_001115"
     CDS             47035..47631
                     /gene="rnhB"
                     /locus_tag="ACHKAU_001115"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO5530226.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47668..51150
                     /gene="dnaE"
                     /locus_tag="ACHKAU_001116"
     CDS             47668..51150
                     /gene="dnaE"
                     /locus_tag="ACHKAU_001116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO5530227.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51163..52122
                     /gene="accA"
                     /locus_tag="ACHKAU_001117"
     CDS             51163..52122
                     /gene="accA"
                     /locus_tag="ACHKAU_001117"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO5530228.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52221..54362
                     /gene="ldcC"
                     /locus_tag="ACHKAU_001118"
     CDS             52221..54362
                     /gene="ldcC"
                     /locus_tag="ACHKAU_001118"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO5530229.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54419..54808
                     /gene="yaeR"
                     /locus_tag="ACHKAU_001119"
     CDS             54419..54808
                     /gene="yaeR"
                     /locus_tag="ACHKAU_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5530230.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54873..56171
                     /gene="tilS"
                     /locus_tag="ACHKAU_001120"
     CDS             54873..56171
                     /gene="tilS"
                     /locus_tag="ACHKAU_001120"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO5530231.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56220..56480)
                     /gene="rof"
                     /locus_tag="ACHKAU_001121"
     CDS             complement(56220..56480)
                     /gene="rof"
                     /locus_tag="ACHKAU_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO5530232.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56467..56667)
                     /gene="yaeP"
                     /locus_tag="ACHKAU_001122"
     CDS             complement(56467..56667)
                     /gene="yaeP"
                     /locus_tag="ACHKAU_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO5530233.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56833..57378
                     /gene="yaeQ"
                     /locus_tag="ACHKAU_001123"
     CDS             56833..57378
                     /gene="yaeQ"
                     /locus_tag="ACHKAU_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO5530234.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57375..57797
                     /gene="arfB"
                     /locus_tag="ACHKAU_001124"
     CDS             57375..57797
                     /gene="arfB"
                     /locus_tag="ACHKAU_001124"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO5530235.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57811..58521
                     /gene="nlpE"
                     /locus_tag="ACHKAU_001125"
                     /gene_synonym="cutF"
     CDS             57811..58521
                     /gene="nlpE"
                     /locus_tag="ACHKAU_001125"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO5530236.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13734 a        14536 c        16307 g        13960 t
ORIGIN      
        1 ttgtcaaatg ctcttgtatg taagacttca cgcaacgtct ccccatcagg aaacgccagc
       61 tccggtgcaa tctcaaacag cggccacagc ataaatccac gattcttcat atcgtagtgc
      121 ggaacggtca ggcgttcggt atttatcact tcattaccaa acagcatgat atcgagatcc
      181 agcgtgcgtg gtccccagcg ttcagcttta cgaacacgac cctgttgcag ttctatgcgc
      241 tgtgtatggt tgagtaactc ttccggggcg agcgaagttt caagcgccac ggcggcgttg
      301 aggtaatcgg gttgatcttg cggccccagc ggtggggtgc ggtaaaacga agaaacggca
      361 agaatgtggc tttcagggat atcgcctaat gctttcaggg cagcattgac ctgctccagc
      421 ggagaggcca gattgctgcc tatggcaata tacgccactg tcatgcggta ccctcacgac
      481 gcggtgcgcg tttgcgtgga cgacgagtac ggcgacgcgg tgacggctct tcatccaact
      541 cgttaagcat ccctttttga tctggtggcg cggaaacctg gaactcaccc caccatttca
      601 ccagacgctg cagttcagcg ttacgttcaa cttcagctcg caaggccaac aggtcataag
      661 ccgcacggaa cttaggatgc tccagcagtt tccatgcgcg tttaccctga cgacgggaca
      721 tacgcaactg caactgccag atatcgcggg ttaacgtcgt cagacgtttc gggattgcca
      781 gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg
      841 tcaggccgct ttcctgggca atcttctgtg ccgtctccag cagtgggtac cagaacatgg
      901 cggcaaacag gaacgccggg ttcacgcgca tatcgttatg gatacgcgta tcggtattct
      961 tcagcacctg ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg aagtagcggg
     1021 taatggtcgg gaacagcggc tggaacagat gatattcaca caacagctta taggtatcgt
     1081 aaccgtagcc cgcttgtagc agtttaagcg attcttcaaa caggcgtgcc ggtgggatat
     1141 cgttcaacag ggtagcgagg cgagggagcg gttctgcggt ttccgggctg atgcgcatac
     1201 ccaatttggc ggcaaaacgt accgcgcgca gcatacgtac cggatcttca cggtagcgcg
     1261 tttccgggtt accaatcaga cggataacgc cgtccttcag atccttcata ccgccaacgt
     1321 aatcacggac ggtaaaatcc gctacgctgt aatagaggct gttgatagtg aaatcgcggc
     1381 gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc
     1441 cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa
     1501 cttcgataat ctccgggcca aacattacat gagccagacg gaaacggcga cccaccaggc
     1561 ggcagttacg gaacagtttg cgcacctgct caggcgtggc gttagtggtt acgtcaaaat
     1621 ctttcggctt tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt
     1681 atcccgcttt gttgagcctg tacattacct tcagggcatt ttcactgata tctttgcggg
     1741 aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg acgggcgact gcctgttcag
     1801 cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag
     1861 tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa tcatagctca
     1921 gcatgacgca tttgagaatg ttgaatttac aattgccgac tcgggcacgg cggtaagccg
     1981 ccagtttttg acggctgact gaaggatttg ctcgacgctg aaatcctgcc agtgtgcttc
     2041 tgcctgctgc cccagaaatt gaagtgccgc gattagtacc gggcgtggat cgcctttcgg
     2101 caacgcaggc gcatgattct gcttggaaag tttagcgcct tgtggattaa gcgccagcgg
     2161 cagatgaatg taatctggca ctttccagcc aaaaagctgg tacagcgaga tttgccttac
     2221 tgttggttca atcagatcag ccccacgcac tatttctgta acgccctgga aatgatcatc
     2281 aaccacaaca gccaggttgt aggcgaacaa cccatcacgg cgatgaatga taaaatcttc
     2341 ccgtgccagt ttttcgtcgg cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac
     2401 cggatgctgc tggcggatac gcactgcggc attgtctggt ccatgatgca acacccggca
     2461 atgaccgtcg taaataccgc caatgctttg aatacgcgca cgcgtacagg tgcagtaata
     2521 acttagtcct tgttcatgta accaggcgag tgcttcacga taggcgtggt gacgttgcga
     2581 ttgccagaga acatcgccgt cccagtgcag accgtaatgt tccagctggc gcaggatagt
     2641 ttctgcggca ccgggaactt cacgaggcgg gtcgatatct tctatgcgta ccagccagcg
     2701 accttgccgg gcgcgagcct gcaaatagct gccgagcgcg gcgatcagag agccaaaatg
     2761 aagctcgccg gaaggagagg gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt
     2821 gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa acgtgatgga acggctgtaa
     2881 ttagccagcc atctgttttt cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc
     2941 ggctgtcggg cgcgcttcca gacggcgaat accaatttca acaccgcagg attcgcagta
     3001 gccgaaatct tcgtcttcca cttttttcag cgtcttctcg atctttttga tcagcttacg
     3061 ctcgcgatcg cggttacgca gttcgaggct gaactcttct tcctgggctg cacggtctac
     3121 cgggtccggg aagttggctg cttcatcctg catatgtgta acggtgcgat cgacttcatc
     3181 cctgagttga ttacgccatg cttccagaat acgacggaag tgcgccagct gggcttcatt
     3241 catatactct tcgcccggct tctcctgata tggttccacc ccagcgatgg cgagaatact
     3301 cagggacgat gttttacggt tttgcccttc ttgcatgttg cttctcctta acacgcacta
     3361 tcgatcccca tgttcggggg aaaaatgagg ccgctataaa tagcagatgc ttttccggat
     3421 agcaattatc taaacgtaac acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg
     3481 accagaatgt aaattaacca gttacttact ttactacaat gtaaccggca gtgatttttt
     3541 aagagccatg ccttcagcag aaatttccgc tttgtaagcc agaatttcta cccccctctg
     3601 ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg
     3661 tgtaatggct gaatgcagca cggcgaaaaa gataaccgca cgctggcctt cagccgctac
     3721 gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc
     3781 ctgttcgttc tccgctaacg taaccgattt cacttcaata tagcagtctg gacgcgaatc
     3841 cgcctgcaac ataaagtcaa tacggctgcg ttctgcgccg tattttactt cgcttttcag
     3901 cgagctatag cctgacagtt ctgaaattga ttcattaagg atagcctctt tcgtcaacct
     3961 gttagcccaa agcgtgttga cgcaaataaa tgcgccgctc tggctttgag ttaattccca
     4021 ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg
     4081 cgttgcacaa ccggtcattg cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc
     4141 gggtgtgatc acatcggcta aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag
     4201 agggggagaa aattccattg cgacaatcct tgttattgcg ttagcgccca gcgttttagc
     4261 ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc
     4321 gcatacgacc agttaaaacc tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat
     4381 aaggtaatat gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt
     4441 gaacggagca tattcgccag ctggattaag ccgcgtggcg gctgacgcat ccctaaccac
     4501 accacacgcg aacgcagcca ttgtccggcg tcatcaagcg tgagtgtgaa accaggttga
     4561 cgaatccgtc cggctaaaag agaaagcgcc ttctctttct cggcgctcac ttcgcctaaa
     4621 aatgccagag tcagatgcaa attatcggcg gcgaccggac gtcccgcctc aggtgggaag
     4681 tgtgtggcgc gccagtggat aatctgttcg cggatttctg caggtaagtc gatagcaaag
     4741 aacagacgtt gcggttcaga catgtgaggc actcggttat gaattaccgc gatgctacaa
     4801 tgtggcgcga agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc
     4861 gtcttacctg aattacttac cgctctcgat tgtgcgccgc aggtattatt aagtgcgccg
     4921 accggggccg ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc cggcattaac
     4981 gggaaaatta tcctgctgga gccgcgtcgt ctggcggcgc gtaacgtcgc gcaacggctg
     5041 gcggagctgc ttaacgaaaa gccaggcgat accgttggct accggatgcg tgcgcaaaac
     5101 tgcgtcgggc cgaatacccg cctggaagta gttaccgaag gcgtgctgac gcggatgatc
     5161 cagcgtgacc cggagttgag cggtgttgga ttggtgatcc tcgatgagtt tcatgagcgc
     5221 agcttgcagg cggatttggc gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat
     5281 gaccttaaac tgctgattat gtcggctacg ctggacaacg accgcttgca gcaaatgctg
     5341 ccagaagcgc ccgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg ccgttattta
     5401 ccactgccca cgcatcagcg ttttgacgaa gccgttgcgg tagccaccgc tgaaatgctg
     5461 cgtcaggaaa gtggatcatt actgttattt ttacctggcg tcggagaaat tcagcgtgtg
     5521 caggaacaac tggcttcgcg catcggcagc gatgtattgc tctgcccgct gtatggcgcg
     5581 ttgtcgctga acgatcagcg aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg
     5641 gtgctggcga ccaatattgc tgaaaccagt ttaaccatcg aaggtattcg tctggtggtg
     5701 gattgtgccc aggagcgtgt ggcgcgtttt gatccgcgca cggggcttac gcgactgatt
     5761 actcaacgcg ttagccaggc atccatgacg cagcgtgccg ggcgcgccgg gcgtctggag
     5821 ccgggtatct gcctgcattt aatcgccaag gaacaagcag aacgcgccgc ggcgcaaagt
     5881 gaaccggaga tattacaaag cgatctttcc ggtttgctga tggaattact gcaatgggga
     5941 tgcagcgatc cggcgcagat gagctggctg gatcaaccgc cagtagtgaa tctactggcc
     6001 gcgaaacgtc tgttacaaat gctgggggca ctggagggtg aacggcttag tgcgcaaggg
     6061 caaaaaatgg cagcgctggg taacgatccg cgtttagcgg caatgctggt tagcgcgaag
     6121 aacgacgacg aagctgctac cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg
     6181 atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt
     6241 agtcagcaac tgttaaaacg cttaaacgta cgtggcggtg aggcagacag ttcgcttatc
     6301 gcgccgctac ttgccggggc gtttgccgat cgcattgctc gtcgccgtgg gcaagatgga
     6361 cgctatcaac tggcaaacgg catgggagcg atgctcgatg ccaacgacgc gctaagccgc
     6421 cacgaatggt tgatcgcacc gttattattg cagggcagcg cctcgccgga tgcgcggatt
     6481 ttactggcgc tgccggtcga tattgatgag ttagtacaac gctgcccgca gctggtacag
     6541 cagtctgaca ctgtggagtg ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg
     6601 caaatcggtc tgctgacggt gaaagtgcag ccgctggcga aaccctcgga agacgagttg
     6661 catcaggcga tgcttaatgg catccgcgat aaagatttaa gcgtgctcaa ctggacggcg
     6721 gaagcggaac agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac
     6781 tggccagcgg ttgatgatga aagtttattg gcgacgctgg aaacgtggct gctgccacat
     6841 atgactggcg tacattcact acgcggcctg aaatcactcg acatttatca ggcactacgc
     6901 ggattacttg attggggaat gcagcaacgt ctggatagtg aattgcctgc gcattacact
     6961 gtgccgacgg gaagccggat cgccattcgt tatcatgaag ataacccgcc cgcgctggcg
     7021 gtgagaatgc aagagatgtt tggcgaggcc accaatccga cgatcgccca ggggcgcgtg
     7081 ccgctggtgc tggagttgct ttcacctgcc caaaggccat tacaaatcac acgagatttg
     7141 agcgccttct ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc
     7201 aaacatgtct ggccggacga cccggcaaat actgcaccga cgcgacggac gaaaaagtat
     7261 tcgtaaggtt ggttttctcc ctctccctgt gggagagggg accgatcgag cacaaatttt
     7321 gagagatatc ttcttctgtc ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa
     7381 tattgcggag aaaaagcatg gccgggaatg accgcgagcc aattggacgc aaagggaaac
     7441 cgacgcgtcc ggtcaaacaa aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg
     7501 acgattatga tgactatgag gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca
     7561 aagggcgtaa gcctcgtggc aaacgcggct ggctatggct gctgttaaaa ctggctatcg
     7621 tttttgccgt gctgatcgcc atttacggcg tttatctcga tcaaaaaatt cgtagccgta
     7681 ttgatggcaa ggtctggcaa ctgcctgcgg cagtttatgg ccgaatggtc aaccttgagc
     7741 cagatatgac catcagcaag aacgagatgg tgaagctgct ggaggcgact cagtatcgtc
     7801 aggtgtcgaa aatgacccgt cctggcgaat ttaccgtgca ggccaacagc attgagatga
     7861 tccgccgtcc gtttgatttc ccggacagta aagaagggca ggtgcgtgcg cgtctgacct
     7921 ttgatggcga tcatctggcg acgatcgtca atatggagaa caaccgtcag ttcggtttct
     7981 tccgtcttga tccgcgtctg atcaccatga tctcctcgcc aaacggtgag cagcgtttgt
     8041 ttgtgccgcg cagtggtttc cctgatttgc tggtggatac tttgctggcg acagaagacc
     8101 gtcattttta cgagcatgac ggcatcagcc tctactccat cggacgtgcg gtgctggcaa
     8161 acctgaccgc cggacgcacg gtgcagggag cgagtacgct gacgcaacag ctggtgaaaa
     8221 acctgttcct ctccagtgag cgttcttact ggcgtaaagc gaacgaagct tacatggcgc
     8281 tgatcatgga cgcgcgttac agcaaagacc gtattcttga gctgtatatg aacgaggtgt
     8341 atctcggtca gagcggcgac aacgaaatcc gcggcttccc gctggcgagc ttgtattact
     8401 ttggtcgccc ggtagaagag ctaagcctcg accagcaggc gctgttagtc ggcatggtga
     8461 aaggagcgtc catctacaac ccgtggcgta acccaaaact ggcgctggag cgacgtaatc
     8521 tggtgctgcg tctgctgcaa cagcaacaga ttattgatca agaactctat gacatgttga
     8581 gtgcacgtcc gctgggtgtt cagccacgcg gtggggtgat ctctccacag ccagccttta
     8641 tgcaactggt gcgtcaggag ctgcaggcga aattaggcga taaggtaaaa gatctctccg
     8701 gcgtgaagat cttcactacc tttgactcgg tggcccagga cgcggcagaa aaagccgccg
     8761 tggaaggcat tccggcactg aagaaacagc gtaagttgag cgatcttgaa actgcgattg
     8821 tggtcgtcga ccgctttagt ggtgaagttc gtgcgatggt cggtggttct gagccgcagt
     8881 ttgcgggcta caaccgtgcg atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag
     8941 cgacttatct gactgcctta agccagccga aaatctatcg tctgaatacg tggattgcgg
     9001 atgcgccaat cgcgctgcgt cagccgaatg gccaggtctg gtcaccgcag aatgatgacc
     9061 gtcgttatag cgaaagcggc agagtgatgc tggtggatgc gttgacccgt tcgatgaacg
     9121 tgccgacggt aaatctgggg atggcgctgg ggctgcctgc ggttacggag acctggatca
     9181 aactgggcgt accgaaagat cagttgcatc cggttccggc gatgctgctg ggggcgttga
     9241 acttaacgcc aatcgaagtg gcgcaggcgt tccagaccat cgccagcggc ggtaaccgtg
     9301 caccgctttc tgcactgcgt tcggtaattg cggaagatgg caaggtgctg tatcagagct
     9361 tcccgcaggc ggaacgcgct gttccggcgc aggcggcgta tctgacacta tggaccatgc
     9421 agcaggtggt acaacgcggt acgggtcgtc agcttggggc gaaatacccg aacctgcatc
     9481 tggcagggaa aacagggact accaacaata acgtagatac ctggtttgca ggcattgacg
     9541 gcagcacggt gaccatcacc tgggtaggcc gtgataacaa ccagccgacc aaactgtatg
     9601 gagccagcgg ggcaatgtcg atttatcagc gttatctggc taaccagacg ccaacgccgc
     9661 tgaatcttgt tccgccagaa gatattgcag atatgggcgt ggactacgac ggcaactttg
     9721 tttgcagcgg tggcatgcgt gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc
     9781 agcagagcga gatgcagcag cagccgtcag gcaatccgtt tgatcagtct tctcagccgc
     9841 agcaacagcc gcaacagcaa cctgctcagc aagagcagaa agacagcgac ggtgtagccg
     9901 gttggatcaa ggatatgttt ggtagtaatt aacatctaag cgtgaaatac cggatggcga
     9961 gttgccatcc ggtaaaataa catcccatct aagatattaa ccctttcttt tcatctggtt
    10021 gtttattaac ccttcaggaa cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc
    10081 gaatattatc ttatctttat aataatcatt ctcgtttacg ttatcattca ctttacatca
    10141 gagatatacc aatggcgcgt tccaaaactg ctcagccaaa acactcactg cgtaaaatcg
    10201 cagttgtagt agccacagcg gtaagcggca tgtctgttta tgcacaggca gcggttgaac
    10261 cgaaagaaga cactatcacc gttaccgttg cacctgcgcc gcaagaaagc gcatgggggc
    10321 ctgctgcaac tattgcggcg cgacagtctg ctaccggcac taaaaccgat acgccgattc
    10381 aaaaagtgcc acagtctatt tctgttgtga ccgccgaaga gatggcgctg catcagccga
    10441 agtcggtaaa agaagcgctt agctacacgc cgggtgtctc tgttggtacg cgtggcgcat
    10501 ccaacaccta tgaccacctg attattcgcg gctttgcggc agaaggccaa agccagaata
    10561 actatctgaa tggcctgaag ttgcagggca acttctataa cgatgcggtc attgacccgt
    10621 atatgctgga acgcgctgaa attatgcgtg gcccggtttc cgtgctttac ggtaaaagca
    10681 gtcctggcgg cctgttgaat atggtcagca agcgtccgac caccgaaccg ctgaaagaag
    10741 ttcagtttaa agccggtact gacagcctgt tccagactgg ttttgacttt agcgattcgt
    10801 tggatgatga cggtgtttac tcttatcgcc tgaccggtct tgcgcgttct gccaatgccc
    10861 agcagaaagg gtcagaagag cagcgttatg ctattgcacc ggcgttcacc tggcgtccgg
    10921 atgataaaac caatttcacc ttcctttctt acttccagaa cgagccggaa accggttatt
    10981 acggctggtt gccgaaagag ggaaccgttg agccgctgcc gaacggtaag cgtctgccga
    11041 cagactttaa tgaaggggcg aagaacaaca cctattctcg taatgagaag atggtcggct
    11101 acagcttcga tcacgaattt aacgacacct ttactgtgcg tcagaacctg cgctttgctg
    11161 aaaacaaaac ctcgcaaaac agcgtttatg gttacggcgt ctgctccgat ccggcgaatg
    11221 cttacagcaa acagtgtgcg gcattagcgc cagcggataa aggccattat ctggcacgta
    11281 aatacgtcgt tgatgatgag aagctgcaaa acttctccgt tgatacccag ttgcagagca
    11341 agtttgccac tggcgatatc gaccacaccc tgctgtccgg tgtcgacttt atgcgtatgc
    11401 gtaatgacat caacgcctgg tttggttacg acgactctgt gccactgctc aatctgtaca
    11461 atccggtgaa taccgatttc gacttcaatg ccaaagatcc ggcaaactcc ggcccttacc
    11521 gcattctgaa taaacagaaa caaacgggcg tttatgttca ggatcaggcg cagtgggata
    11581 aagtgctggt caccctaggc ggtcgttatg actgggcaga tcaagaatct cttaaccgcg
    11641 ttgctgggac gaccgataaa cgtgatgaca aacagtttac ctggcgtggt ggtgttaact
    11701 acctgtttga taatggtgta acaccttact tcagctatag cgaatcgttt gaaccttctt
    11761 cgcaagttgg gaaggatggt aatattttcg caccgtctaa aggtaagcag tatgaagtcg
    11821 gcgtgaaata tgtaccggaa gatcgtccga ttgtagttac tggtgccgtg tataatctca
    11881 ctaaaaccaa caacctgatg gcggaccctg agggttcctt cttctcggtt gaaggtggcg
    11941 agatccgcgc acgtggcgta gaaatcgaag cgaaagcggc gctgtcggcg agtgttaacg
    12001 tagtcggttc ttatacttac accgatgcgg aatacaccac cgatactacc tataaaggca
    12061 atacgcctgc acaggtgcca aaacacatgg cttcgctgtg ggctgactat accttctttg
    12121 acggtccgct ttcaggtctg acgctgggca ccggtggtcg ttatactggc tccagctatg
    12181 gtgatccggc taactccttt aaagtgggaa gttatacggt cgtggatgcg ttagtgcgtt
    12241 atgatctggc gcgagtcggc atggcgggct ccaacgtggc gctgcatgtt aacaacctgt
    12301 tcgatcgtga atacgtcgcc agctgcttta acacttatgg ctgcttctgg ggcgcagaac
    12361 gtcaggtcgt tgcaaccgca accttccgtt tctaatttct cttttggggc acggatttcc
    12421 gtgcccattt cacaagttgg ctgttatgca ggaatacacg aatcattccg ataccacttt
    12481 tgcactgcgt aatatctcct ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt
    12541 aacctttcct gccgggaaag tgaccggtct gattggtcac aacggttctg gtaaatccac
    12601 tctgctcaaa atgcttggcc gtcatcagcc tccgtcagaa ggggagattc ttcttgatgc
    12661 ccagccgctg gaaagctgga gcagcaaagc gtttgcccgc aaagtggctt atttgccgca
    12721 gcagcttcct ccggcagaag ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc
    12781 gtggcatggc gcgctggggc gctttggtgc ggcagatcgc gaaaaggtcg aggaagctat
    12841 ctcgctggtt ggcttaaaac cgctggcgca tcggctggtc gatagtctct ctggcggcga
    12901 acgtcagcgg gcgtggatcg ccatgctggt ggcgcaggat agccgttgtc tgttgctcga
    12961 cgaaccgact tcggcgctgg atatcgccca ccaggttgat gtgctggcgc tggtgcaccg
    13021 tttaagtcag gaacgtggcc tgacggtcat tgccgtgctg cacgatatca atatggcggc
    13081 acgctactgt gattatctgg tcgccctgcg cggtggtgaa atgattgctc agggaacgcc
    13141 tgcggaaatt atgcgcggcg aaaccctcga aatgatttat ggcatcccga tgggtatttt
    13201 gccgcatccg gcgggtgctg cacctgtgag ttttgtttat tgatgagcgg cttacctctt
    13261 atttcgcgcc gtcgactgtt aacggcgatg gcgctttctc cgttgttatg gcagatgaat
    13321 accgcccacg cggcggctat tgaccccaat cgtattgtgg cgctggagtg gttgccggtg
    13381 gaattactgc tggcgctcgg catcgtgcct tacggcgtgg cggataccat caactatcgc
    13441 ctgtgggtca gcgaaccacc attgccggac tcagtgattg acgtcggttt gcgtacagaa
    13501 cctaaccttg aactgctgac cgaaatgaaa ccgtcgttta tggtctggtc ggcaggatat
    13561 ggcccttcac cagaaatgct ggcacgtatt gcgccgggtc gcggatttaa cttcagtgac
    13621 ggcaaacagc cgttggcgat ggcgcgtaaa tcgctgacgg aaatggcaga tttacttaac
    13681 ctgcaaagcg cagcagaaac gcatttagcg cactatgaag actttatccg cagcatgaaa
    13741 ccccgctttg tgaagcgtgg tgcacgcccg ttactgctga cgacgcttat cgatccgcgc
    13801 catatgctgg tcttcggtcc aaacagcttg ttccaggaaa ttctcgatga gtacggcatc
    13861 ccaaatgcct ggcaagggga aaccaacttc tggggcagta ccgccgtcag tatcgatcgt
    13921 ctggcggcgt ataaagacgt tgatgtgctc tgttttgatc acgacaacag caaagacatg
    13981 gatgcgctaa tggcaacccc gctgtggcag gccatgccgt ttgtccgtac agggcgcttt
    14041 cagcgcgtac ccgcagtctg gttttatggt gcaacgcttt cggcaatgca ctttgtgcgt
    14101 gttctggata acgccatcgg aggtaaagcg tgagtaaacg aattgcgctt ttcccggcgt
    14161 tattgctggc gctgttagtg attgtcgcta cggcgctaac ctggatgaac ttctcacagg
    14221 cgctgccgcg cagtcagtgg gcgcaggccg cctggtcgcc ggatattgac gtcatcgagc
    14281 agatgatttt tcactacagc ttgttgccgc gtctggcgat ttcgctgctg gtgggcgcag
    14341 gcctggggct ggtgggcgtg ctgtttcagc aagtgctgcg taatccgctg gcggaaccga
    14401 cgacacttgg cgttgctaca ggcgcgcaac tgggaattac cgtcaccaca ctttgggcga
    14461 tcccaggtgc tatggcgagc cagtttgccg cactggtggg cgcttgtgtc gttggcttaa
    14521 tcgtctttgg cgtcgcgtgg gggaaacggc tttcgccggt tacgcttatt ctcgcggggc
    14581 tggtggtgag cctttattgc ggggcaatta atcagctact ggttatcttc catcacgacc
    14641 agctgcaaag catgttcctg tggagcaccg gaacgctgac gcaaaccgac tggggcggcg
    14701 ttgagcgttt atggccgcag ttgctgggcg gcgtgatgtt gacgttattg ctacttcgcc
    14761 cgttaaccct gatggggctt gatgatggcg tggcgcgcaa tctcgggctg gccttgtcgc
    14821 tcgcgcgtct ggcagcgctg tcgctggcga ttgtcatcag tgcgctgctg gtgaacgctg
    14881 tggggattat cggctttatc gggttgttcg cgccgctgct ggcaaaaatg ctgggggcgc
    14941 ggcgtctgct gccacgactg atgctggcgt cgctgattgg ggcgctgatc ctctggcttt
    15001 ccgatcaaat catcctctgg ctgactcgcg tgtggatgga agtgtccacc ggttcggtca
    15061 ctgcgttgat cggtgcgccg ctgctactgt ggctgttgcc gcgtttacgc agcattagcg
    15121 cgccggatat gaaggtcaac gatcgtgtcg cggctgaacg ccaacatgtg ctggcgtttg
    15181 ccctcgcggg cggcgtgctg ctgttgatgg ctgtggtggt ggcgctgtcg tttggtcgtg
    15241 atgcgcacgg ctggacgtgg gcgagcgggg cgttgcttga ggatttgatg ccctggcgct
    15301 ggccgcgaat tatggcggcg ctgtttgcgg gcgtcatgct ggcggtggcg ggctgtatta
    15361 ttcagcggct gaccggaaac ccgatggcaa gcccggaagt gctggggatt agctctggcg
    15421 cagcgtttgg cgtggtgttg atgctgtttc tggtgccggg taatgccttt ggctggctgt
    15481 tacctgcagg cagtctcggc gcggcggtga cgctgttgat cattatgatc gccgccggcc
    15541 gcggtggatt ttccccacac cgcatgttac tggcggggat ggcgttaagc accgcgttca
    15601 ccatgctttt gatgatgttg caggcaagtg gtgacccgcg aatggcgcaa gtgctgacct
    15661 ggatttccgg ttcgacctac aacgcgaccg atgcgcaggt ctggcgcacg ggaattgtga
    15721 tggtgatttt gctggcgatt accccgctgt gccgccgctg gctgaccatt ttaccgctgg
    15781 gtggtgatac cgcccgagcc gtaggaatgg cgctgacgcc gacgcgaatt gcgctgctgc
    15841 tgttagcggc ttgcctgacg gcgaccgcga cgatgactat tggaccgttg agttttgttg
    15901 gtttaatggc accgcatatt gcgcggatga tgggctttcg acggacgatg ccacacatcg
    15961 taatttcggc gctggtgggt ggtttactgc tggtgttcgc tgactggtgt gggcggatgg
    16021 tgctgtttcc attccagatc ccagcagggc tgctgtcgac ctttatcggc gcgccatatt
    16081 ttatctattt gttgagaaag cagagccgtt aatagaatgc ctcgcccggt gggcgaggca
    16141 aaatcatcac aacttcgcaa acacccgtcg tgcagcatcg atggtgttat tgatatcttc
    16201 catgctgtgc gccacggaca taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc
    16261 ttcgtccagc atcatatgga agaaacgctt aaagcgttcc acgtcacagg ccatcacatc
    16321 ctgatagcac gtcacggact cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg
    16381 gttaacgacc agcggaattc cggcttcttc tgccgcttcc agcagacctt ctgccagacg
    16441 tgttgtcagc tcatccagcg tttcgtgaac gcccggctgc gcgacttcat tcagacaggc
    16501 gaaacccgct gccatcgcaa ttgggttacc ggaaagcgta cccgcctgat agaccggacc
    16561 cgtcggggcc agcgcatcca ttacatcacg acgaccaccg aatgcgccta ccggcattcc
    16621 accgccgatg attttgccca ggcaggtgag atccggttcc acaccgtaat aatcctgtgc
    16681 gccagctagc gctacgcgga agccagtcat tacttcatcg atgatcagca atgcgccaaa
    16741 ttcgtcgcac agcgcgcgca gacctggcag gaactctggc agcggtggaa cgcagttcat
    16801 attgcctgcc accggctcga cgataataca ggcaatctct tgcgggtatt gctcaaacgc
    16861 ggcgcgtaca gaagccagat cgttataagt acaggttaag gtatgtttgg cgaaatctgc
    16921 cggaacaccc ggcgagtttg gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag
    16981 caggcagtca gcgtgaccgt ggtaacaacc ttcaaattta atgattttgt cgcgaccggt
    17041 aaaaccacgg gccaggcgga tggcgctcat cgtcgcttca gtgccggagt tcaccatgcg
    17101 caccatatcc atggtcggga ccagttcggt caccagttgc gccattttca cttccatttc
    17161 ggtcggtgca ccaaagctta aaccacgctc ggcggcttca atcacggcat tgcggatagc
    17221 cggatggtta tggcccagca ccatcggccc ccaggaaccg acataatcga tataggcttt
    17281 gccatcaaca tcgtacagat aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc
    17341 cacgccagta aaggcgcgaa caggggagtt cacaccgcca gggatcagct cgcgcgctgc
    17401 gctgtaaaga ttttcagact tactcatgga gggttcctga ttcgtagaaa aagtgaatgg
    17461 ctgctattct atgttattca taacaagtta aatactcgtc aaacatcagg ctgcttgtac
    17521 tggtgcaatc ctgatttctt agagtataaa agttttgtgc atttgaaaca ttacgctttg
    17581 caaaggattt tcatggaacg tgcgagtaaa atgccgtcat cttatttgta tgaccaataa
    17641 gtgatcattg gatgaaaact gatactccct ctttagaaac accgcaggcc gcgcgcctgc
    17701 gacgcagaca actgattcgc caacttcttg agcgcgataa aaccccgtta gccattttgt
    17761 ttatggcggc agtcgtcggc acgcttgtcg ggctggcagc ggttgctttt gacaaaggtg
    17821 tcgcctggtt gcagaaccaa cgtatggggg cgctggtaca tactgctgat aattatccgc
    17881 ttctgttaac cgtcgctttt ctctgttcgg cggtgctggc gatgttcggc tactttttgg
    17941 tgcgcaaata cgcgccggaa gcaggtggtt cggggatccc ggaaattgaa ggggcgctgg
    18001 aagatcaacg tcccgttcgc tggtggcgtg tgttgccggt gaaattcttt ggcgggctgg
    18061 ggacactcgg cggaggcatg gtgctagggc gcgaaggacc aaccgtgcag atcggcggca
    18121 atatcggtcg catggtgctt gatattttcc gcctgaaagg tgatggagct cgccatacgc
    18181 tgctggcaac cggtgctgct gcggggctgg ctgcggcctt taacgcgccg ctggcgggta
    18241 ttttgtttat tatcgaagag atgcgtccgc agtttcgcta tacgttaatt tcgattaaag
    18301 cggtatttat tggcgtcatt atgtcgacca ttatgtaccg gatttttaat catgaagttg
    18361 cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg ctttatctga
    18421 tcctcggtat tatttttggc attttcggcc ctatttttaa taaatgggtg ctggggatgc
    18481 aggatttgct gcaccgtgtg cacggcggca atattaccaa atgggtactg atgggtggtg
    18541 cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc agcaacgtcg ggcggcggtt
    18601 ttaacctgat tcctatcgct accgcgggga atttcagcat gggaatgctg gtgtttattt
    18661 tcgtcgctcg ggtcattacc accttactct gcttctcttc cggcgcgccg ggcggtattt
    18721 ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg
    18781 agctgtttcc gcaatatcac ctggaggcgg ggacgtttgc tattgccgga atgggggcat
    18841 tactggcggc atctattcgc gcgccgttaa cggggatcat tctggttctg gagatgaccg
    18901 ataactacca gctcattttg ccaatgatta ttaccggtct tggcgcaaca ctattagcgc
    18961 aatttaccgg cgggaaaccg ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg
    19021 aagctatgca actggcgcga agcaaggccg catcagccag cgagaatact tgaacgaaat
    19081 accagggtat tagataatgg cgattattat tgggttagaa tttgcccaat tgccgatgtc
    19141 gtttggagca aaatatgagt gatgacgtag cactgccgct ggagtttacc gacgcagcag
    19201 ccaacaaagt taaaagcctg atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt
    19261 atatcaccgg tggcggttgc agcggcttcc agtatggttt cacctttgat gatcaggtga
    19321 acgaaggcga tatgaccatc gaaaaacagg gcgttggctt ggtggttgat ccgatgagcc
    19381 tgcaatatct ggtcggcggt tccgttgact ataccgaagg actggaaggt tctcgtttca
    19441 tcgtgaccaa cccgaacgcg aaaagcacct gcggttgcgg ttcttccttt agcatctaat
    19501 ctgttgttca tgtgccggat aaactatccg gcacatcttc tctcttcaac gcccattctc
    19561 atccagcgca aacgtcggta gcttaagatg ccaacgaata gccgccagcc gaatcaagag
    19621 cgtcacgacc atgcccatca tactggctgt ttccagtggt acggaaaatg tgtaataagc
    19681 cgtagcgtgg acaataccgc cgataataca ggcagttgcg tagatttctg tacgtaaaat
    19741 catggggatt tcgcgggcca gaacatcacg aatgatcccg ccgccaacgc cagtaatgac
    19801 gcccatacaa accgcgatta acggaccggc ttccgcatta aaggctttat tcacgccaat
    19861 gccgacaaac accgccagac caacggcgtc cagcaccggc aacatccatt ttggtaagcg
    19921 tcttggctgg cgcaccagca cgatggtcag catgctggtt accattgcaa cgaccagatc
    19981 ggtgggatct ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc gaattgtccc
    20041 gccgcctaca gcggtaacca cgcccagtac cagaacacca aaagggtcca tacgcaattt
    20101 tccggctaac aaaacgccgg agatggcaaa taccgctgtg ccgactatat ccagccaata
    20161 gacgagcatt gttcaatccc cactgagcac ctgaaaaggt caggcactaa tctacctgtg
    20221 aaagcgcatt acagagctgt tgtgcagcga ggataatacg tgggcttgca cgttcaaacc
    20281 agtcactcgt gagaggaata acgggaattt tgagctgttc accccagtat tgtttgattt
    20341 taggaatttg gtccggtccg cctgtaatga caatcgcctg tggcgagcgt gctaacacct
    20401 gttcgcggct aacttgcggc cagggaaccc ggctgtcttt aaagatgttt tctccgccac
    20461 aaacttcgag tacctggttc tgaatcgact cttttccact ggtaaatggc ggattaatgc
    20521 cgaattgcag aaaaacacgt tttttaggtt tatcagcata ttgcgctttc aattgcgcgt
    20581 actgatccag cagggattgc gcggcttgtt cggccttgtc tggttgcgga ctccaggggg
    20641 ccagttgacg taacgcattg gcaatttgtt caatgcttgt cgcatcgacc cacatcactt
    20701 ttattcccag cgaagccagc tggtcaacct gccgctcggc attacctcca cgccaggcaa
    20761 tcaccagatc gggtttcagc gcgacaatgc gttccagatt catcccctgc caggtggaaa
    20821 cctgctcaat cttttgcgct tgtggaggat agtcggaata gctgctgacc ccaaccggcg
    20881 tgatcccggc ggcaaaggca agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg
    20941 cggcgttgag ccacagtggc gcaagaaaag acagggcgac cagcgccctg aacagtgact
    21001 tagccatgtg caagtttctg caccagtgac tcaaccatca ggctggactg tttagcggca
    21061 acagccagga actcatcgaa gctaagatga gactgttgat cggccacgtc ggagatggcg
    21121 cgtacgacaa caaacgggac gttgaaattg tggcagacat gggcgattgc cgtcgcttcc
    21181 atctctacag caatggcctg tgggaagttg tggcggattt tcgccagacc aacagaaccg
    21241 ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag cgttaagatt cagttcggca
    21301 atgcaggcct cagcggcagc gatcagttta tcgtcagctt taaagcctgc cggacagcct
    21361 ggtaactgac cgtattcata accaaatgcc gtgacatccg cgtcgtgata acgtgcttcg
    21421 tccgagacaa cgatatcgcc cactttcaac gttggtgcca ggccaccggc agaaccggtg
    21481 ttaataatca catctggctt gcagtgttcc aacagcaaag tggcacccag cgccgcagcg
    21541 actttaccga tgcccgattt cagaagcgca acctcggttc cattcagttg gccggtatag
    21601 atttcgcaac cgccgagact gatagtttga cgtttttcga ttttgtcacg cagcagcgta
    21661 acttcttctt ccattgcacc aatgatgccg attttcatag atttactcgc gataagcccg
    21721 atttgaaggc atagtttacc atgcgcttac ggggaagcgt atttctcacg cgggagagga
    21781 catggcacag attgatttcc gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca
    21841 gggcgttaaa accgaacatg agatcctgcg gatcttcgag agcgatcgcg ggcgtatcat
    21901 caactctccg gcaattcgtc gtctgcaaca aaagacgcag gtttttccac tggagcgcaa
    21961 tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc cagcaggtgg ggcgctacat
    22021 cgccaaagaa attttaagcc gtctgaaaga gcttaaattg ctggaagcat acggcctgga
    22081 tgaactgacc ggaccttttg aaagcattgt tgagatgtca tgcctgatgc acgatatcgg
    22141 caatccgccg tttggtcatt ttggcgaagc agcgataaat gactggtttc gccaacgttt
    22201 gcacccggaa gatgccgaaa gccagcctct gactgacgat cgctgcagcg tggcggcact
    22261 acgtttacgg gacggggaag aaccgcttaa cgagctgcgg cgcaagattc gtcaggactt
    22321 atgtcatttt gaggggaatg cacaaggcat tcgtctggtg catacattga tgcggatgaa
    22381 tctcacctgg gcacaggttg gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg
    22441 cgaaacgcct gagacacatc actatttaat gaaaaagccg ggttattatc tttctgaaga
    22501 agcctatatt gcccggttgc gtaaagaact taatttggcg ctttacagtc gttttccatt
    22561 aacttggatt atggaagctg ccgacgacat ctcctattgt gtggcagacc ttgaagatgc
    22621 ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg aagcgtgggg
    22681 ccagcatgag aaaggttcgc tcttttcgct ggtggttgaa aatgcctggg aaaaatcacg
    22741 ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac gggtaaacac
    22801 cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc ctgcgatttt
    22861 cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg atcttcttaa
    22921 gctatataaa aatgtcgctg taaaacatgt gtttagccat ccagatgtcg agcagcttga
    22981 attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt tattaagcct
    23041 gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt tccctattga
    23101 atcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag
    23161 taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc gttgccgcct
    23221 gctgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat accgacgtct
    23281 gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt ttaccttttg
    23341 cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac agcaattttg
    23401 cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac tgagtgcact
    23461 ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg agacttcttc
    23521 agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc
    23581 agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa
    23641 tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac cgttccagag
    23701 ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac agaaattcat
    23761 ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca ccaacaacca
    23821 cgttgttgat aacgcgacgg tgattaaagt ccagctgggc gatggtcgta agttcgacgc
    23881 gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg attcagatcc agaacccgaa
    23941 aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg attacaccgt
    24001 agcgattggt aacccgtttg gcctgggaga gacggtaact tccgggattg tctctgcgct
    24061 ggggcgtagc ggcctgaatg ccgaaaacta cgaaaacttc atccagaccg atgcagcgat
    24121 caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga tcggtatcaa
    24181 caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta tcccgagcaa
    24241 catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac gcggtgagct
    24301 gggtattatg gggactgagc tgaattccga actggcgaaa gcgatgaaag ttgacgccca
    24361 gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag cgggcattaa
    24421 agcgggtgat gtgatcacct cactgaacgg taagccaatc agcagctttg ccgcactgcg
    24481 tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttgc tgcgcgacgg
    24541 taagcaggtc aacgtgaatc tggaactgca gcagagcagc cagaatcagg ttgattccag
    24601 ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag atcagggcgt
    24661 ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga agaaaggtga
    24721 tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc gtaaagttct
    24781 tgacagcaaa ccgtctgtac tggcactgaa cattcagcgc ggcgacagct ccatctacct
    24841 gttaatgcag taatctccct caaccccttc ctgaaaacgg gaaggggttc tccttacaat
    24901 ctgtgaactt caccacaact ccatacatct tcatcatcct ttaggcattt gcacaatgcc
    24961 gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg gctggcatct
    25021 tgataccaaa atggcgcagg atatcgtggc acgtaccatg cgcatcatcg ataccaatat
    25081 caacgtaatg gatgcccgtg ggcgaattat cggcagcggc gatcgtgagc gtattggtga
    25141 attgcacgaa ggtgcattgc tggtactttc acagggacga gtcgtcgata tcgatgacgc
    25201 ggtggcacgt catctgcacg gtgtgcggca agggattaat ctaccgttac ggctggaagg
    25261 tgaaattgtc ggcgtaattg gcctgacagg tgaaccagag aatctgcgta aatatggcga
    25321 actggtctgc atgacggctg aaatgatgct ggaacagtcg cggttgatgc acttgttggc
    25381 tcaggatagc cgtttgcggg aggaactggt gatgaacctg attcaggcag aggagaatac
    25441 tcccgcactt actgaatggg cgcaacggct ggggatcgat ctcaatcaac cgcgagtggt
    25501 ggctattgtt gaggtcgaca gcggtcagct tggtgtggac agcgcaatgg cggagttaca
    25561 acaactgcaa aacgcgctga ctacgcccga gcgtaataat ctggtggcga ttgtctcgct
    25621 aaccgaaatg gtggtgttga aaccggcgtt gaactctttt gggcgctggg atgcagaaga
    25681 tcatcgtaag cgagttgaac aactgattac ccgcatgaaa gagtacggcc agctgcgttt
    25741 tcgcgtttca ctgggcaact attttaccgg tcctggcagt attgcccgat cctatcgtac
    25801 ggcgaaaacg acgatggtgg tgggtaaaca gcggatgcca gaaagtcgct gctattttta
    25861 tcaggatctg atgttacctg tgttactcga cagtttgcgt ggcgactggc aggccaacga
    25921 actggcgcga ccgctggcgc ggctgaaagc gatggacaat aacggcttgc tgcgacgaac
    25981 gctggcggcg tggtttcgtc acaatgtgca accgctggca acgtcaaagg cgttgtttat
    26041 tcatcgtaat accctggagt atcggcttaa tcgtatatcg gaactgaccg ggcttgattt
    26101 gggcaatttt gatgacaggt tgctgctgta tgtggcgtta caactggatg aagagcggta
    26161 ggttatgcgt taaggttgag gcggcgctgg ctcattcccc tcagcccgaa ggggagaggg
    26221 gaatgcccga attaaccgtc aattcgggat tatttacgcg ttagtttttc cagatcggct
    26281 tcaatctcgc tgatcttatt ggttacgact gactccaggt gacgtaagtc gtcgaggatc
    26341 ttacgcttaa gatcaacttc gctgcggtca cgctggcaga tttgatcaag ctcatcaatg
    26401 atataccgta gattcgggct gatttcctgg acttctttat aaccctgacc cacaccatca
    26461 gcgacgaccg ttttacgctg acgcggatat ttaaacttaa cgctcttggc gaaaaactcg
    26521 cctttgtctt tctggaaata gattttcaga atatcgttgt tggcttcctg ccggaggctg
    26581 taacgatcaa tttcttcagg attggtaata cccagacttt tcagattgtc gtacatagcg
    26641 ttacctcaaa atgagtcagt aaattgtgct tattttagca tttggcctgg cccgcccggc
    26701 tcgactgtga tcgcagaaag cctttttctt ccagacttct atcatggcgt aaaaatcaaa
    26761 aaattacctg ctttattgtg gtgataaaat tcacgttcta caccagttcc agcatcgtta
    26821 cttccgggcg gcagttcaga cgcaaaccat acaaactgcc cacgccacgg gttgtgtaga
    26881 tgtgtcgttc gccaaaggca tttaatccgg cgacgtaacg tttatcttcg acaggggcaa
    26941 aaggttcacc aaccaacggt acgcgcagtt gcccgccgtg agtatggccg cacagcatca
    27001 gatcccaggg ttcatcacgc atgacttctt tgctgtcggg attatgcgcc agcaccagtc
    27061 ttggcagatt ggcttcgctg gcgggaggcg gtttgcattg tccggcccat aagtcaccgg
    27121 tgcctaccag ttcgaattgc ctgttcggcg tggcgatcac cgtagcctgg ttaaacaaca
    27181 ccgtgatgcc cgctgatttc aacgtctcgc caattaagtg atttttttct gtaccaacag
    27241 ggcgatcgtg gttgccgaag caggcaaacg tcggcgcaca ttcggcaagg ggggagagga
    27301 catcactaaa cgccgaaaaa ttcagcggca tatcaaataa tacgtaatcg ccgcccagta
    27361 atatcaaatc gggcttttgt tctatgccaa gagcaatcgc gtcagaaatc aggcttaaag
    27421 gaacaaaacg agagtaatgg agatcggcca gaaaaagaat tttgaatggt gctgcgttgt
    27481 ctttaaaaaa ggcgaggcgg tgacggatta attcaaacca gcctggctcc cagtaatgca
    27541 tataaccgaa acctgagctc gtggctatcg tcgcggcagt agcctgcaac aatcggcggc
    27601 gtgaaatcat cgctcatccc tgcaaaaaaa atcgggcagc gtcgtgctgc ccgtgtgcat
    27661 acttttagtc gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt
    27721 caactttctt aacgataacc gcacagtaga ggctgtattt gccatctttt gacggcagat
    27781 tacctgaaac aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac
    27841 ggtcgtaaat acgggtgctc tgaccaatgt atacgcccat ggaaatgacg gaaccttctt
    27901 cgacaatcac cccttcaacc acttcagagc gtgcgccaat gaagcagtta tcttcaatga
    27961 tggttgggtt agcctgcagc ggttccagca cgccgccgat gcccacgcca ccggaaaggt
    28021 ggacgttttt accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatggtgc
    28081 cttcatcaac atatgcgccg atgttgacgt aagacggcat cagcacggtg ttacgggcaa
    28141 taaacgcacc ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctggaaac
    28201 gtgcttcgtc gtagtcggcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc
    28261 cttcgatcac ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact
    28321 gatgcgtcac ccactgaccg tcaatttttt ccgctacacg cagtgcaccg gaatccagca
    28381 gggcgatcac ctgatttacc gcttcgcggg taacggtgtc tgcgttggct ggcgtgatct
    28441 cggcacggcg ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct
    28501 tttcatatca gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct
    28561 gatgcacttc ctgctgcaac tcgttattaa gtgcacgccg gtcagcggtg gcaattatga
    28621 ataaatcttc tactcgctcg ccaatggttg taattcgggc accatgaagc gaaattccca
    28681 gatcggcaaa aattttcccg actctcgcca gcagtcctgg ctggtcgagc gcgataagct
    28741 caaggaacga tttgcggtcg gtatgggtcg gcaaaaacgt tacttcggtt tcaacagtaa
    28801 aatggcgtaa tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca
    28861 gtacttgctc cagaccaaac cgaataacct catgacgatc tgcggacagc gggctgccat
    28921 cgggttccag cacgataaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg
    28981 cgtcgtgaac acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg
    29041 ggcggtccgg gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca
    29101 ataccagcgg tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat
    29161 ttgggctatg gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct
    29221 cttcgtcgat gttatccatg cgcagtagtg ccagtgcctg gagttggtga tgacgaaccc
    29281 gttcgcgcat atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat
    29341 agagttcgcg caacagactt tgcttccagc tattccacag cgtttcgttg gtggcgcaaa
    29401 tgtcagccac cgtcaggcat accagataac gaagtcggtt ttctgtttgc acttcttcgg
    29461 caaactgctt gatgacttcc gggtcctgaa tatcgcggcg ttgggcggtc accgacatca
    29521 acaggtgctg gcgaaccagc caggcaacca gctgtgtttc gcgcgagtta agcccgtgga
    29581 gttcggcaaa atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt
    29641 tggcgatatc gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc
    29701 agacgtccac acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt
    29761 tcagcatcac gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca
    29821 tctgcccgac gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca
    29881 ttggcaatag cccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacaattttc
    29941 gtgcttccgg aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc
    30001 gcagcgtggt ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac
    30061 gcaagatggc ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac
    30121 cgcgtagctg aaactcatcg tcgagtggac gtggtttttc gtcggcggga agggcgagga
    30181 tggcttcatc gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc
    30241 ggaagtaatc cttcatcatc cgctcgaccg gttcgttgcc ttcaccactg taattcagac
    30301 gctgagcgac gctaagttgg cgatcgaata acagacgatt atcgtaacgg ctgacgacca
    30361 gatgcagggc aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct
    30421 ccgctgaggt taagaagcca aacccaacca tttcatccag cgatgttgcg ccaaaatgac
    30481 ggcgggccac ccattgcaga gtgtggatat cgcgcaagcc gccagggcta cttttgatgt
    30541 ctggttcaag attgtagctg gtgccgtggt aacgctgatg gcgctggttc tgttcttcaa
    30601 ctttcgccgc gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca
    30661 gttcgaggaa gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga
    30721 cggttaaatc cgataaccct tccagcatgc actcttcaag cgtgcgcaca ctatgaccga
    30781 cttccagctt tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt
    30841 ccgggagctt tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc
    30901 cacgaccgta gccaccgacg gcgaccaatg ccaggtcggc aatctggctg aatcccgctt
    30961 caatccataa tcgttgcagg agctggtcga taaactcggt gcgcgcctca atcaactgtt
    31021 ctgcagagat cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg
    31081 cttttatccc accgacggtt aattcatcac ggggccagac gcatggattt tgcggttgac
    31141 cgggcagggt ggggagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc
    31201 acccataaga ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg
    31261 tcgtgcgaga ttatcgccgg gatggtgtca tccttgcgta gcgtcagaat ttcgcagccg
    31321 ttatcagtca ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt
    31381 accgtccagc catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctcg
    31441 atggtgaacg tcatcccagg tttcagtacg acgttggttt cacgggagtc atagtgcagc
    31501 acctgcggtt cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg
    31561 gagaagcctt ctgcttcgac aaatttctga atcgccgcac cgatttcgcg cagattaatg
    31621 cctggtttga ccatgcgcag cgccaggtac aggctttctt gcgtgatgcg gcacagacgt
    31681 tcgcccatga tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca
    31741 tctttgatta cggtgacatc aatgttaacg atatcgccat ctttcagcag cttcgcatcg
    31801 tccgggatac cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg
    31861 tgatagccga ggcaggcaga aaccgtgtgt tgttcattaa caatgtaatc attacagatg
    31921 cgatccagct cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact
    31981 tcggcagcca gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag
    32041 atagccatta attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt
    32101 caatggtatc acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg
    32161 aacgttttct ataatagaaa attcaacgtc tgatgctgta cacagcgcca acaattattg
    32221 gtgtccatga cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac
    32281 agactggacg gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt
    32341 ccggggtgcc ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat
    32401 atagaggttt taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac
    32461 ttcggtcacc agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt
    32521 aacaaagttc acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct
    32581 gaactgaaca agattgcttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct
    32641 gcaagcgaag cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc
    32701 tggctgggcg gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa
    32761 gacctggaaa ctcagtctca ggacggtact ttcgaaaagc tgaccaagaa agaagcgctg
    32821 atgcgcactc gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc
    32881 ggtctgccgg acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa
    32941 gcaaacaacc tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt
    33001 gttgacttcg ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc
    33061 gctgttgctg caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa
    33121 agcttcgtag aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag
    33181 aaaggcggca agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga
    33241 gcgaagacaa cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta
    33301 gtacacgttt ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg
    33361 tttaatgccg ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc
    33421 tggtaaaaga gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga
    33481 ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta
    33541 aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg
    33601 gcaactacgg catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg
    33661 gtttccaggc gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg
    33721 ttgaagttct gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg
    33781 agaacatcaa cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc
    33841 acggtgcgcg tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac
    33901 acatcgctat gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg
    33961 ctgaagtggt agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc
    34021 cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt
    34081 ctctgaccgg tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag
    34141 agcataacgc tgaagtgact ggcttcatcc gcttcgaagt gggtgaaggc atcgagaaag
    34201 ttgagactga ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcaaaaa
    34261 ggagccgcct gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg
    34321 gggctgcgct gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca
    34381 gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa
    34441 gttgagtggc gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga
    34501 tcgtatggct caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat
    34561 tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt
    34621 gggcgaccac atggggatgc tggcgaccgt aatgaacggc ctggcaatgc gtgatgcact
    34681 gcaccgcgcc tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga
    34741 cagctacagc tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc
    34801 tgccggtaca ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga
    34861 aattgaagcc gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ccgctgatcc
    34921 ggcgaaagat ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa
    34981 agagctgaaa gtcatggacc tagcggcctt cacgctggct cgtgaccata aattaccgat
    35041 tcgtgttttc aatatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga
    35101 agggacttta atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa
    35161 gtatcgcggg ggcgttagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg
    35221 ccagcgcgca gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgctcggggc
    35281 caaaatggca aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag
    35341 ggctatactt agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc
    35401 aaggattcgt aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg
    35461 cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct
    35521 gctggatggc attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag
    35581 cgtaacggta gaagattccc gtacactgaa aatcaacgtg tttgatcgtt caatgtctcc
    35641 ggccgttgaa aaagcgatta tggcgtccga tcttggcctg aacccgaact ctgcgggtag
    35701 cgacatccgt gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat
    35761 cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt aacgtgcgtc gtgacgcgaa
    35821 cgacaaagtg aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc
    35881 tcaggacgat gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc
    35941 agacaaagaa gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt
    36001 agatccttgc ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt
    36061 tcatgaagca actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg
    36121 tggtgcgcca taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca
    36181 ctcgcatggt agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag
    36241 cgagtgcgaa acttcttaaa acgatgctac agcaacaggg tagccgcacc gaagtcttaa
    36301 gtgggcaaca agccgcttgc gatatggcag cgcttgagga tgttgatcag gtgatggcag
    36361 ccattgttgg cgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca
    36421 ttttgctggc caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa
    36481 agcagagcaa agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt
    36541 taccgcaacc tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt
    36601 ccattttact taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa
    36661 caatgacgcc ggatcaagcc tgccgtcatc cgaactggtc gatggggcgt aaaatttctg
    36721 tcgattcggc taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta
    36781 acgccagcgc cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg
    36841 tgcgctatca ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa
    36901 ttgcccacac catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt
    36961 gcaaactaag tgcgttgaca tttgccgcac cggattatga tcgttatcca tgcctgaaac
    37021 tggcgatgga ggcgttcgaa caaggtcagg cagcgacgac agcattgaat gccgcaaacg
    37081 aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga
    37141 atttatccgt actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat
    37201 ctgttgatgc gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag
    37261 gataatccgg ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct
    37321 gcgccatctg atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg
    37381 cttttttttg tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa
    37441 acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg
    37501 tcatgttgcg atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg
    37561 tgcctttggg cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa
    37621 caacggtatt gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc
    37681 gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag
    37741 tctgcaccga cataacgtgc gtctgcgtat tattggcgat accagtcgct ttaactcgcg
    37801 tttgcaagaa cgtattcgta aatctgaagc gctaacagcc gggaatagcg gtctgacgct
    37861 gaatattgcg gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc
    37921 tgaaaaggtg cagcaaggaa acctgcaacc agatcagata gatgaagaga tgctaaacca
    37981 gcatgtctgt atgcatgaac tggcccctgt agatttagta attaggactg ggggggagca
    38041 tcgcattagt aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt
    38101 tctctggccc gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg
    38161 agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt
    38221 gctgaagtat cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt
    38281 tctgttgccg ccggtggggt tcgccatcgt aacgctggtg gtctgcatgc tggcagcgtg
    38341 ggaatgggga cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt
    38401 atgcgggtta ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca
    38461 tcaaccgctg gttgaaatct cactttgggc ttcgttgggt tggtggattg tcgcgctatt
    38521 gctggtgctg ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct
    38581 tatttttggc gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg
    38641 gcactacgac gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt
    38701 atggggcgct gactctggcg catatatgtt tggcaaattg tttggtaaac ataagctggc
    38761 accgaaggtt tctccgggta aaacctggca aggctttata ggtggactag cgaccgcagc
    38821 ggtaatctca tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact
    38881 catttgctct attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt
    38941 taagcgtgaa gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt
    39001 agatcgtatt gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt
    39061 attcaggacg ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc
    39121 atcgttgcac tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg
    39181 cgttgtggtg ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga
    39241 actgataagc tcggcaccga atatgttatc gccctgatcc cgctgggcgg ttatgtcaaa
    39301 atgctggatg agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat
    39361 aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt
    39421 gctatctttg cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt
    39481 ggtgaaatag cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta
    39541 aaagccgtag atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat
    39601 aaaattggcg atgaaagcac caccattaca gtagcgccat ttggcagcga ccaacggcgg
    39661 gatgtaaagc tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct
    39721 tcgctgggga ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcaacca
    39781 aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt
    39841 cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc
    39901 ttagcgttag aaatcgaaag gcagggaagt cccttgtctt tgacattaat cccggagagt
    39961 aaaccgggta atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg
    40021 ccagatgagt ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg
    40081 gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt
    40141 gatgtgaaac tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca
    40201 gcggaactcg gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg
    40261 ataattaacc tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc
    40321 gaaaagatca agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc
    40381 tcgattctgc tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga
    40441 gagttagtta ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc
    40501 tgctgtttag cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt
    40561 tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgcacag
    40621 gcgacacggt taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca
    40681 actttgagga tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac
    40741 gtccgaccat tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga
    40801 agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg
    40861 ccgatatcga gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg
    40921 taaaagctgt cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg
    40981 aaggtgtgtc agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg
    41041 acgaactgat ctctcatttc caactgcgtg acgaagtgcc gtggtggaac gtggtaggcg
    41101 atcgtaaata ccagaaacag aaactggcgg gcgaccttga aaccctgcgc agctactatc
    41161 tggatcgcgg ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata
    41221 aaaaaggtat ttacgtcacg gtgaacatta ccgaaggcga tcagtacaag ctttctggcg
    41281 ttgaagtgag cggcaacctt gccgggcact ccgctgaaat tgagcagctg actaagatcg
    41341 agccgggcga gctgtataac ggcaccaaag tgaccaagat ggaagatgac atcaaaaagc
    41401 ttctcggtcg ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg
    41461 ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc tacgtgcgta
    41521 agatccgttt tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc
    41581 agatggaagg tgcatggctg ggaagcgatc tggtcgatca gggtaaggag cgtctgaatc
    41641 gtctgggctt ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc
    41701 aggtagatgt cgtctacaag gtaaaagagc gcaacaccgg tagcttcaac ttcgggattg
    41761 gttacggtac tgaaagtggc gtgagcttcc aggctggtgt gcagcaggat aactggttag
    41821 gtacaggtta tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac
    41881 tgtcggtaac caacccgtac ttcactgtag atggtgtaag cctcggtggt cgtctcttct
    41941 ataatgactt ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta
    42001 cagacgtgac gttgggcttc ccgatcaacg aatataactc gctgcgtgca ggtctgggtt
    42061 atgtacataa ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact
    42121 ctatgggtga acatccgagc acctctgatc aggataatag cttcaaaacg gacgacttca
    42181 cgttcaacta tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt
    42241 cacgtgtcaa cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag
    42301 tgacgttaga cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg
    42361 ggcgtacccg ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga
    42421 acttctatgc cggtggttcc agcaccgtgc gtggcttcca gtccaataca attggtccga
    42481 aagcagttta cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg
    42541 cgactcagga cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca
    42601 tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta
    42661 actcggttcg tacttccttc ttctgggata tgggtaccgt ttgggataca aactgggatt
    42721 ccagccaata ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg
    42781 gtatcgcatt acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt
    42841 tcaaaaagta cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt
    42901 aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata
    42961 agatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt
    43021 attgtgaaaa agtggttatt agcggcaggt ctcggtttag cactggcaac ttctgctcag
    43081 gcggctgaca aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt agcgcagaaa
    43141 accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt
    43201 atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc gggcagcgat
    43261 cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag
    43321 gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt
    43381 atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca
    43441 aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag
    43501 gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa
    43561 ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca
    43621 ggtcacatta cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg
    43681 tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg
    43741 aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc
    43801 gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta
    43861 tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc
    43921 ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg
    43981 aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca
    44041 gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca
    44101 cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat
    44161 cgcggcgcgc tggatgacac tattattggc aatggcgtga tcattgataa ccagtgccag
    44221 attgcacata acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg
    44281 ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat
    44341 atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact
    44401 gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc
    44461 gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag
    44521 gttaatcaac aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt
    44581 cttacgattg cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta
    44641 tcttgactac taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc
    44701 gtttcccgtt cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg
    44761 cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga
    44821 ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta
    44881 aaagcgtagg aaaactggaa ccgggtgagc tgtactactt cgctggtatt gacgaagcgc
    44941 gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa
    45001 cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt cgatggtaaa gtagtttgcg
    45061 aagcaacgat gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct
    45121 ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcaat tggcgcgaac gcacacattg
    45181 gtcctttttg tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc
    45241 acgttgtcgt gaatggtcat actaaaattg gccgcgataa tgagatttat cagttcgcct
    45301 ccatcggcga agttaaccag gatctgaaat atgctggcga accgacccgt gtggaaatcg
    45361 gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg
    45421 gattgacgaa ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt
    45481 gtacggtagg taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg
    45541 ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg
    45601 cgcacgtgat ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgtcattg
    45661 cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat
    45721 tcagccgtga ggcgattacc gctatccgca atgcgtataa gctgatttat cgtagcggta
    45781 aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga
    45841 aagcctttac cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgaaca
    45901 gcgtccatta acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg
    45961 tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc
    46021 acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg
    46081 cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa
    46141 gcgttttggc gaactgaagc cagatatttt tgttggtatt gatgcgcctg acttcaatat
    46201 tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc attcattacg tcagtccatc
    46261 cgtctgggcg tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct
    46321 cgcatttctg cctttcgaaa aagcgtttta tgacaaatac aatgtaccgt gccgctttat
    46381 cggtcatacc atggctgatg ccatgccgtt agatccagat aaaaatgccg cccgtgatgt
    46441 gctggggatc cctcacgatg cccactgcct ggcgttgcta ccagggagcc gtggtgcaga
    46501 agttgaaatg cttagtgccg atttcctgaa aacggcccag cttttgcgcc agacatatcc
    46561 ggatctcgaa atcgtggtgc cactggtgaa tgccaaacgc cgcgagcagt ttgaacgcat
    46621 caaagctgaa gtcgcgccag acctttcagt tcatttgctg gatgggatgg gccgtgaggc
    46681 gatggtcgcc agcgatgcgg cgctactggc gtcgggtacg gcagccctgg agtgtatgct
    46741 ggcgaaatgc ccgatggtgg tgggatatcg catgaagcct tttaccttct ggttggcgaa
    46801 gcggctggtg aaaactgatt atgtctcgct gccaaatctg ctggcgggta gagagttagt
    46861 caaagaatta ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct
    46921 gttggcgaac gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca
    46981 gatccgctgc aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc
    47041 gaatttgttt atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg
    47101 ttagttggcg cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg
    47161 ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga agagatcaaa
    47221 gagaaagcgt tgagctggag tctgggccgc gcggaacccc acgaaatcga cgagctgaac
    47281 attcttcatg cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg
    47341 gaatatgtgt tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg
    47401 gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt ctatcctggc gaaagtgacg
    47461 cgtgacgccg aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacac
    47521 aaagggtacc caaccgcttt tcatctggaa aaactggctg aacacggcgc gaccgaacac
    47581 catcggcgca gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt
    47641 cgagattaag taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg
    47701 cacagcgact actcgatgat cgatggcctg gccaaaaccg caccgttggt aaaaaaggcg
    47761 gcggcgttgg gtatgccagc actggcgatc accgatttca ccaacctttg tggtctggtg
    47821 aagttctacg gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaac
    47881 gtccagtgcg acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat
    47941 accggctatc agaatctgac gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc
    48001 gccgggccga tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt
    48061 tccggcggac gcatgggcga cgtcggacgc agtcttttgc gtggtaacag agcgctggta
    48121 gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc
    48181 cgcaccggca ggccggatga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg
    48241 cgcggtttgc ccgtcgtggc gaccaacgac gtgcgcttta tcgacagcag cgactttgac
    48301 gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg
    48361 cgtaactatt cgccgcagca atatatgcgt agcgaagagg agatgtgtga gctgtttgcc
    48421 gacatccccg aagcccttgc caacaccgtt gagatcgcca aacgctgtaa cgtaaccgtg
    48481 cgtcttggtg aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat
    48541 ctggtcaagc gtgcaaaaga gggcctggaa gagcgtctgg cctttttatt ccctgacgag
    48601 gaagaacgtg ttaagcgccg cccggaatat gacgaacgtc tggagactga acttcaggtt
    48661 atcaaccaga tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg
    48721 aaagataacg gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc
    48781 tacgcgctga aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc
    48841 cttaacccgg aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc
    48901 gatcaggtta tcgagcacgt agcggacatg tacggtcgtg atgcggtatc gcagatcatc
    48961 accttcggta caatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat
    49021 ccgtacggct ttgtcgatcg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg
    49081 gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt
    49141 aaggcgctga tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac
    49201 gccggtgggg tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttactgcgat
    49261 gaagagggca aacatccggt cacccagttt gataaaagcg acgttgaata cgccggactg
    49321 gtgaagttcg acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg
    49381 atcaacaagc ggcgggcgaa gaatggcgag ccgccgctgg atatcgctgc gatcccgctg
    49441 gatgataaga aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt
    49501 gaatcgcgcg gcatgaagga cctgatcaag cgtctacaac ctgactgctt cgaagatatg
    49561 atcgccctag tggcactgtt ccgccccggt ccgttgcaat cagggatggt ggataacttt
    49621 atcgaccgta aacatggtcg tgaagagatc tcctatccgg acgtacagtg gcagcatgaa
    49681 agcctgaaac cggtactgga gccaacctac ggcattatcc tgtatcagga acaggtcatg
    49741 cagattgcgc aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgtcgtgcg
    49801 atgggtaaga aaaagccgga agagatggct aagcaacgtt ctgtatttgc tgaaggtgca
    49861 gaaaagaacg gaatcaacgc tgaactggcg atgaaaatct tcgacctggt ggagaaattc
    49921 gctggttacg gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg
    49981 ttatggctga aagcacacta tcctgcggag tttatggcgg cggtaatgac cgccgatatg
    50041 gacaacaccg agaaggtggt gggtctggtg gatgagtgct ggcggatggg gctgaaaatc
    50101 ctgccaccag atataaactc cggtctttac catttccacg tcaacgacga cggcgaaatc
    50161 gtgtatggta ttggcgcgat caaaggggtc ggtgaaggtc cgattgaggc catcatcgaa
    50221 gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc
    50281 aaaaagttga accgtcgcgt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt
    50341 gggccacacc gcgcggcgct gatgaactcg ctgggtgatg cgttaaaagc ggcagatcaa
    50401 cacgcgaaag cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg
    50461 gaacaaattg aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat
    50521 ggggaacgtg aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa
    50581 gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt
    50641 aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc
    50701 aatcgtatcg gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt
    50761 actgacgccc tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc
    50821 ggacaggtca gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg
    50881 gatattgacg aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg
    50941 caaattgatg accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg
    51001 acaattccag tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc
    51061 gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt
    51121 tcggagcagg tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt
    51181 gattttgaac agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc
    51241 cgtcaggatg agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa
    51301 agcgtagaac tgacgcgtaa aatcttcgcc gatcttggtg catggcagat tgcgcaactg
    51361 gcacgccatc cacagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt
    51421 gacgaactgg ctggcgaccg cgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc
    51481 cgtcttgacg gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa
    51541 aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg
    51601 atgcaaatgg ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct
    51661 tatccgggcg tgggcgcaga agaacgtggt cagtctgaag ccattgcacg caacctgcgt
    51721 gaaatgtctc gcctcggtgt accggtagtt tgtacggtta tcggtgaagg tggttctggc
    51781 ggcgcgctgg cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc
    51841 gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga gcgctgacaa agcgccgctg
    51901 gcagctgaag cgatgggtat catcgctccg cgtctgaaag aactaaaact gatcgactcc
    51961 atcatcccgg aaccgctggg tggtgctcac cgtaacccgg aagcgatggc ggcatcgttg
    52021 aaagcgcaac tgctggcgga tctggccgat ctcgacgtgt taagcactga agatttaaaa
    52081 aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa
    52141 aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa
    52201 ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt
    52261 ataaagatga gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta
    52321 tctggccaca aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg
    52381 gcgtgatttt tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta
    52441 atgaatatct cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc
    52501 aggatatgcg gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg
    52561 ccattcgtat gcgtcagtac accgacgaat atcttgataa cattacaccg ccgttcacga
    52621 aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg
    52681 gcggcaccgc atatcaaaaa agcccggttg gctgcctgtt ttatgatttt ttcggcggga
    52741 ataccctcaa ggctgatgtc tctatttcgg tcaccgagct tggttcgttg ctcgaccaca
    52801 ccgggccaca cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt
    52861 atatcgttac caacggaaca tcgacgtcga acaaaattgt gggtatgtac gccgcgccat
    52921 ccggcagtac gctgttgatc gaccgcaatt gtcataaatc gctggcgcat ctgttgatga
    52981 tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attcttggtg
    53041 ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccaccacgc
    53101 aggcacaatg gccggttcat gcggtgatta ccaactccac ctatgatggc ttgctctaca
    53161 acaccgactg gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg
    53221 tgccgtacac ccattttcat ccgatctacc aggggaaaag tggtatgagc ggcgagcgtg
    53281 ttgcgggaaa agtgatcttc gaaacgcaat cgacccacaa aatgttggcg gcgttatcgc
    53341 aggcttcgct gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta
    53401 tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttgag acggcggcgg
    53461 cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc
    53521 attttcgcaa agaggtccag cggttgcggg aagagtctga cggttggttt ttcgatatct
    53581 ggcaaccgcc gcaggtggat gaagccgaat gctggcccgt tgcgctaggc gaacagtggc
    53641 acggctttaa cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt
    53701 tgacaccggg gatggacgaa cagggcaata tgagcgagga ggggatcccg gcggcgctgg
    53761 tggcaaaatt cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc
    53821 tgtttctctt tagtattggt atcgataaaa ccaaagcaat gggattattg cgtgggttga
    53881 cggaatttaa acgctcttac gatctcaacc tgcggatcaa aaatatgcta cccgatctct
    53941 atgctgaaga tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc
    54001 ataagctgat tcgtaaacac gatcttcccg gtttgatgtt gcgggcattc gatactttgc
    54061 cggaaatgat catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa
    54121 ccattgcgct ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac
    54181 cgggcgtacc gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg
    54241 attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc
    54301 acggcgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat
    54361 aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat
    54421 gctgggttta aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa
    54481 agctttctac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg
    54541 cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt
    54601 tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt
    54661 tagcgttgat gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga
    54721 agccatccgt gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg
    54781 gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt
    54841 gccgggcatt gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc
    54901 tcacctcacg ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc
    54961 atcagttggt gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc
    55021 atcacggttt aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac
    55081 agtggcaggt gccgctggtg gtcgaacgcg tgcaacttgc gcaagaagga ctgggcattg
    55141 aggcccaggc gcggcaggcg cgttatcagg catttgcccg caccttgttg cccggtgaag
    55201 tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac ttttctgctg gcgctaaaac
    55261 gcggtagcgg ccctgccggg ctttcggcta tggcggaagt ttcggagttt gccggaacac
    55321 ggcttatccg cccgttgctc gcccgcacgc ggggagaact ggagcagtgg gcattggcgc
    55381 atggtttacg ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc
    55441 tgcgcctgcg cgtagtgccg ttattgcagc agcgttggcc gcattttgcg gaagcaacgg
    55501 cccgcagcgc cgcactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg
    55561 atttagcaca ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga
    55621 gtgatgcccg ccgcgcggcg attatccgtc gctggctggc agggcagaat gcaccaatgc
    55681 cttcccgcga cgcgctggtg cggatctggc aggaagtggc gctggcgcgg gaagatgcct
    55741 ccccctgttt acgcttgggc gcgtttgaaa tccgccgcta tcagtcgcaa ctgtggtgga
    55801 ttaaatccgt caccgggcaa agtgaaacca ttgtgccgtg gcagacgtgg ctacaaccgc
    55861 tggaattacc ggcggggctg ggaagcgtac agcttactgc gggaggcgat attcgccctc
    55921 cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcggc aggattgctg catattgtcg
    55981 ggcgcaacgg cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc
    56041 tacgtgacac cacgccactg ctgttttatg gcgaaacgct gattgcggcg gcaggggtat
    56101 ttgtgacgca agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa
    56161 cgcttagtta agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat
    56221 caggactcgc tcaccaccac cgtaccgatt tccgggtggc taaagctggt aattttatcc
    56281 agacgtagct cacgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg
    56341 gagactaaat cgctggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc
    56401 attaaatgat gctggcaggc gagctcaaga ttatcgtaat catcacaatt gattggttga
    56461 tacgtatcat tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt
    56521 ttccctgaca gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac
    56581 gcagcccagc gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt
    56641 gcgtattaac tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg
    56701 gatgcaagtc taagcctacg aagataaact ctgtttcgca aggtgactat accacactca
    56761 tgtctgcaat atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa
    56821 aggagttttc ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat
    56881 ctcgaccgca accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc
    56941 caggagcgta tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa
    57001 tttacccgtg gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg
    57061 ggcattgatt tgtggattga gctggggctg ccggatgagc ggcggattaa gaaagcctgc
    57121 acccaggccg cagaagtggc gctgtttacc tataatagtc gggcggcgca aatctggtgg
    57181 cagcaaaatc agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat
    57241 gaacaactgg cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt
    57301 caggatggcg tgatctggtt atcggacgat aagaataatc tggaagttaa cttaaccgcc
    57361 tggcaacaac cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg
    57421 agatcaccgc cattcgtgcg cagggcgcgg gcgggcagca tgttaataag acctcaacgg
    57481 ccattcatct gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc
    57541 tactcgccgc cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg
    57601 aataccgcag tcaggaactg aaccgcgaag ccgcgctagc ccggctggtg gcagtgatta
    57661 aagatttaac aacagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag
    57721 agcgcaggct ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac
    57781 gcagcggtcg ggaataaaaa gaaggaatag atggtgaaaa aagcgatagt gacagcgatg
    57841 gctgtaatca gcctctttac tctgatggga tgtaataacc gggccgaagt cgatacgctt
    57901 tctccagcac aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctgccg
    57961 tgtgctgatt gcgaaggaat cgaaacctct ctgttcctcg aaaaagacgg aacatgggtg
    58021 atgaatgagc gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca
    58081 tgggcgcgaa ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat
    58141 cgggcgaaag gcgatgcgct ggagatgctc gatcgtgaag gcaatccgat tgaatcgcag
    58201 ttcaactata cgctggaacc ggcacaatct agtttaccta tgacgccgat gaccctgcga
    58261 ggcatgtatt tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt
    58321 ttcatggtag cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt
    58381 gaaaaaccgg tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact
    58441 ggtgcgccaa ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat
    58501 tgcagtagtt tggggcagta aacctgctta aggcaga
//
LOCUS       DAZFJF010000016        56647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFJF010000016 DAZFJF010000000
VERSION     DAZFJF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.016"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..384
                     /locus_tag="ACHKAU_001126"
     CDS             <1..384
                     /locus_tag="ACHKAU_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5530237.1"
                     /translation="LGIYLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKK
                     AGNTKLGEFGNYSNDWQTLELVFTASSATVTPKLNGVAGPAFQVIKDSLTLGLNALTL
                     TDITKNATYGVEIESLVLEINSPAA"
     gene            408..875
                     /locus_tag="ACHKAU_001127"
     CDS             408..875
                     /locus_tag="ACHKAU_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5530238.1"
                     /translation="MLKTNSLRKGMIHGCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGAPQAEHLQEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            868..1503
                     /locus_tag="ACHKAU_001128"
     CDS             868..1503
                     /locus_tag="ACHKAU_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5530239.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            1515..2081
                     /locus_tag="ACHKAU_001129"
     CDS             1515..2081
                     /locus_tag="ACHKAU_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5530240.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDANPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            2078..2428
                     /locus_tag="ACHKAU_001130"
     CDS             2078..2428
                     /locus_tag="ACHKAU_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO5530241.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            2432..3328
                     /locus_tag="ACHKAU_001131"
     CDS             2432..3328
                     /locus_tag="ACHKAU_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5530242.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            3321..3851
                     /locus_tag="ACHKAU_001132"
     CDS             3321..3851
                     /locus_tag="ACHKAU_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO5530243.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATHDGEVITIYPDD"
     gene            3854..4567
                     /locus_tag="ACHKAU_001133"
                     /pseudo
     CDS             3854..4567
                     /locus_tag="ACHKAU_001133"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            5132..5986
                     /locus_tag="ACHKAU_001134"
     CDS             5132..5986
                     /locus_tag="ACHKAU_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5530244.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     ISGAHAPAFIRSRRDTPDANWSPWAQLYTSAHPPEEFYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAVAYPSGVIPDMRGWTIKGKPGSGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGNHSHNIPVGHTGAGNGVSAGYNAAL
                     GTGTTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNATGNEENTVKNIA
                     FNYIVRLA"
     gene            5986..6564
                     /locus_tag="ACHKAU_001135"
     CDS             5986..6564
                     /locus_tag="ACHKAU_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5530245.1"
                     /translation="MTFRMSEHSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDSLFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVNTAVAADVEWPVAPQ"
     gene            complement(6608..7180)
                     /locus_tag="ACHKAU_001136"
     CDS             complement(6608..7180)
                     /locus_tag="ACHKAU_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO5530246.1"
                     /translation="MLSYLMAIHFVLFGDSTNLKNFWKYEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDMPGSRVKIGSNVDIGANCCIIGLSRKIGDN
                     VKIGAMSFINKDIPSNCTYITKKSGVVLYK"
     gene            complement(7337..7825)
                     /locus_tag="ACHKAU_001137"
     CDS             complement(7337..7825)
                     /locus_tag="ACHKAU_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5530247.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKSKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
     gene            complement(7838..10645)
                     /locus_tag="ACHKAU_001138"
     CDS             complement(7838..10645)
                     /locus_tag="ACHKAU_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5530248.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARKWYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAVGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPAASRNREPAVSRFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(10632..10787)
                     /locus_tag="ACHKAU_001139"
     CDS             complement(10632..10787)
                     /locus_tag="ACHKAU_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO5530249.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(10796..11161)
                     /locus_tag="ACHKAU_001140"
     CDS             complement(10796..11161)
                     /locus_tag="ACHKAU_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5530250.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            complement(11216..11728)
                     /locus_tag="ACHKAU_001141"
     CDS             complement(11216..11728)
                     /locus_tag="ACHKAU_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO5530251.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(11728..12912)
                     /locus_tag="ACHKAU_001142"
     CDS             complement(11728..12912)
                     /locus_tag="ACHKAU_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO5530252.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            13070..14170
                     /locus_tag="ACHKAU_001143"
     CDS             13070..14170
                     /locus_tag="ACHKAU_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO5530253.1"
                     /translation="MTDTTMQLLSQSTDPVKMPDFDILAEGETLSGVAERLMSLSLTD
                     NRGFEADLLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSSNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPKKEKSSSREGDYMEGAEGNVFVLR
                     KTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSDIDNQDW
                     IIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            14570..15709
                     /locus_tag="ACHKAU_001144"
     CDS             14570..15709
                     /locus_tag="ACHKAU_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014606071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HFO5530254.1"
                     /translation="MGNNKVKLGRNDKCWCNSGLKYKKCHLGRDRETPVSKAEALKFS
                     KSLSQRKACYAPASMHEDCDKRIVQAHTISKSSSLKAIADSSNHVMGVIMNLASLMRN
                     NGKWLPEKIGINQASTFTGFCAVHDRILFSCLENEKFTGTDEQCFALMFRSLSKEIYA
                     KEGGVLSSDFAKNADKGKLQVEQYYIQKLIDQHQSGLNAAIIELNNLKTSLDRILLSR
                     QFSEIESVIFTFAEPLPIVVSSILSPERDFDGTKIQDLSDLTVSAEQVCFNAFSCGGK
                     GYVVFSWLRTSTIIRRFIRTLIKIPANRMFNTLLYFFFTKAENTYSSPEWWDRLSDKQ
                     RENIGNMIMSGVEFFGRPTSQVDHSVDYNAAALEEITCSNNEICS"
     gene            15996..16256
                     /locus_tag="ACHKAU_001145"
     CDS             15996..16256
                     /locus_tag="ACHKAU_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5530255.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            16447..16587
                     /locus_tag="ACHKAU_001146"
     CDS             16447..16587
                     /locus_tag="ACHKAU_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5530256.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLRGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(16776..17057)
                     /locus_tag="ACHKAU_001147"
     CDS             complement(16776..17057)
                     /locus_tag="ACHKAU_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530257.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(17596..17682)
                     /locus_tag="ACHKAU_001148"
     tRNA            complement(17596..17682)
                     /locus_tag="ACHKAU_001148"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17646..17648),aa:Leu,seq:taa)
     gene            complement(17695..17768)
                     /locus_tag="ACHKAU_001149"
     tRNA            complement(17695..17768)
                     /locus_tag="ACHKAU_001149"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17734..17736),aa:Cys,seq:gca)
     gene            complement(17823..17898)
                     /locus_tag="ACHKAU_001150"
     tRNA            complement(17823..17898)
                     /locus_tag="ACHKAU_001150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17863..17865),aa:Gly,seq:gcc)
     gene            complement(18050..18598)
                     /gene="pgsA"
                     /locus_tag="ACHKAU_001151"
     CDS             complement(18050..18598)
                     /gene="pgsA"
                     /locus_tag="ACHKAU_001151"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO5530258.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(18655..20487)
                     /gene="uvrC"
                     /locus_tag="ACHKAU_001152"
     CDS             complement(18655..20487)
                     /gene="uvrC"
                     /locus_tag="ACHKAU_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO5530259.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(20484..21140)
                     /gene="uvrY"
                     /locus_tag="ACHKAU_001153"
     CDS             complement(20484..21140)
                     /gene="uvrY"
                     /locus_tag="ACHKAU_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO5530260.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            21282..21398
                     /locus_tag="ACHKAU_001154"
     CDS             21282..21398
                     /locus_tag="ACHKAU_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5530261.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            21436..21612
                     /gene="yecU"
                     /locus_tag="ACHKAU_001155"
     CDS             21436..21612
                     /gene="yecU"
                     /locus_tag="ACHKAU_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO5530262.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTA
                     NFHCNMTRKINEYA"
     gene            21599..21823
                     /gene="yecF"
                     /locus_tag="ACHKAU_001156"
     CDS             21599..21823
                     /gene="yecF"
                     /locus_tag="ACHKAU_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO5530263.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(21891..22613)
                     /gene="sdiA"
                     /locus_tag="ACHKAU_001157"
     CDS             complement(21891..22613)
                     /gene="sdiA"
                     /locus_tag="ACHKAU_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO5530264.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(22843..23595)
                     /gene="tcyN"
                     /locus_tag="ACHKAU_001158"
     CDS             complement(22843..23595)
                     /gene="tcyN"
                     /locus_tag="ACHKAU_001158"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO5530265.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(23592..24260)
                     /gene="tcyL"
                     /locus_tag="ACHKAU_001159"
     CDS             complement(23592..24260)
                     /gene="tcyL"
                     /locus_tag="ACHKAU_001159"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO5530266.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(24275..25261)
                     /gene="dcyD"
                     /locus_tag="ACHKAU_001160"
     CDS             complement(24275..25261)
                     /gene="dcyD"
                     /locus_tag="ACHKAU_001160"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO5530267.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(25366..26166)
                     /gene="tcyJ"
                     /locus_tag="ACHKAU_001161"
     CDS             complement(25366..26166)
                     /gene="tcyJ"
                     /locus_tag="ACHKAU_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5530268.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(26254..26805)
                     /gene="fliZ"
                     /locus_tag="ACHKAU_001162"
     CDS             complement(26254..26805)
                     /gene="fliZ"
                     /locus_tag="ACHKAU_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO5530269.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(26851..27570)
                     /gene="fliA"
                     /locus_tag="ACHKAU_001163"
     CDS             complement(26851..27570)
                     /gene="fliA"
                     /locus_tag="ACHKAU_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO5530270.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(27735..29198)
                     /locus_tag="ACHKAU_001164"
     CDS             complement(27735..29198)
                     /locus_tag="ACHKAU_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO5530271.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            29453..30865
                     /gene="fliD"
                     /locus_tag="ACHKAU_001165"
     CDS             29453..30865
                     /gene="fliD"
                     /locus_tag="ACHKAU_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5530272.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            30880..31290
                     /gene="fliS"
                     /locus_tag="ACHKAU_001166"
     CDS             30880..31290
                     /gene="fliS"
                     /locus_tag="ACHKAU_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5530273.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            31290..31655
                     /gene="fliT"
                     /locus_tag="ACHKAU_001167"
     CDS             31290..31655
                     /gene="fliT"
                     /locus_tag="ACHKAU_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO5530274.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            31733..33220
                     /gene="amyA"
                     /locus_tag="ACHKAU_001168"
     CDS             31733..33220
                     /gene="amyA"
                     /locus_tag="ACHKAU_001168"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5530275.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(33254..33667)
                     /gene="yedD"
                     /locus_tag="ACHKAU_001169"
     CDS             complement(33254..33667)
                     /gene="yedD"
                     /locus_tag="ACHKAU_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO5530276.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            33854..35059
                     /gene="yedE"
                     /locus_tag="ACHKAU_001170"
     CDS             33854..35059
                     /gene="yedE"
                     /locus_tag="ACHKAU_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO5530277.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            35056..35289
                     /gene="yedF"
                     /locus_tag="ACHKAU_001171"
     CDS             35056..35289
                     /gene="yedF"
                     /locus_tag="ACHKAU_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO5530278.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            35396..36064
                     /gene="yedK"
                     /locus_tag="ACHKAU_001172"
     CDS             35396..36064
                     /gene="yedK"
                     /locus_tag="ACHKAU_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO5530279.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(36459..36773)
                     /gene="fliE"
                     /locus_tag="ACHKAU_001173"
     CDS             complement(36459..36773)
                     /gene="fliE"
                     /locus_tag="ACHKAU_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5530280.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            36988..38646
                     /gene="fliF"
                     /locus_tag="ACHKAU_001174"
     CDS             36988..38646
                     /gene="fliF"
                     /locus_tag="ACHKAU_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5530281.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            38639..39634
                     /gene="fliG"
                     /locus_tag="ACHKAU_001175"
     CDS             38639..39634
                     /gene="fliG"
                     /locus_tag="ACHKAU_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5530282.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            39627..40313
                     /gene="fliH"
                     /locus_tag="ACHKAU_001176"
     CDS             39627..40313
                     /gene="fliH"
                     /locus_tag="ACHKAU_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5530283.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            40313..41686
                     /gene="fliI"
                     /locus_tag="ACHKAU_001177"
     CDS             40313..41686
                     /gene="fliI"
                     /locus_tag="ACHKAU_001177"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5530284.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            41705..42148
                     /gene="fliJ"
                     /locus_tag="ACHKAU_001178"
     CDS             41705..42148
                     /gene="fliJ"
                     /locus_tag="ACHKAU_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5530285.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            42145..43272
                     /gene="fliK"
                     /locus_tag="ACHKAU_001179"
     CDS             42145..43272
                     /gene="fliK"
                     /locus_tag="ACHKAU_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5530286.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            43377..43841
                     /gene="fliL"
                     /locus_tag="ACHKAU_001180"
     CDS             43377..43841
                     /gene="fliL"
                     /locus_tag="ACHKAU_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO5530287.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            43846..44850
                     /gene="fliM"
                     /locus_tag="ACHKAU_001181"
     CDS             43846..44850
                     /gene="fliM"
                     /locus_tag="ACHKAU_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO5530288.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            44847..45260
                     /gene="fliN"
                     /locus_tag="ACHKAU_001182"
     CDS             44847..45260
                     /gene="fliN"
                     /locus_tag="ACHKAU_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO5530289.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            45263..45628
                     /gene="fliO"
                     /locus_tag="ACHKAU_001183"
     CDS             45263..45628
                     /gene="fliO"
                     /locus_tag="ACHKAU_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO5530290.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            45628..46365
                     /gene="fliP"
                     /locus_tag="ACHKAU_001184"
     CDS             45628..46365
                     /gene="fliP"
                     /locus_tag="ACHKAU_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5530291.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            46375..46644
                     /gene="fliQ"
                     /locus_tag="ACHKAU_001185"
     CDS             46375..46644
                     /gene="fliQ"
                     /locus_tag="ACHKAU_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO5530292.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            46653..47438
                     /gene="fliR"
                     /locus_tag="ACHKAU_001186"
     CDS             46653..47438
                     /gene="fliR"
                     /locus_tag="ACHKAU_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5530293.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            47728..48351
                     /gene="rcsA"
                     /locus_tag="ACHKAU_001187"
     CDS             47728..48351
                     /gene="rcsA"
                     /locus_tag="ACHKAU_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO5530294.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQHPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(48395..48583)
                     /gene="dsrB"
                     /locus_tag="ACHKAU_001188"
     CDS             complement(48395..48583)
                     /gene="dsrB"
                     /locus_tag="ACHKAU_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO5530295.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            48746..48973
                     /gene="yodD"
                     /locus_tag="ACHKAU_001189"
     CDS             48746..48973
                     /gene="yodD"
                     /locus_tag="ACHKAU_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO5530296.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            49271..50086
                     /gene="yedP"
                     /locus_tag="ACHKAU_001190"
     CDS             49271..50086
                     /gene="yedP"
                     /locus_tag="ACHKAU_001190"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO5530297.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(50083..51777)
                     /gene="dgcQ"
                     /locus_tag="ACHKAU_001191"
     CDS             complement(50083..51777)
                     /gene="dgcQ"
                     /locus_tag="ACHKAU_001191"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO5530298.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(52015..52197)
                     /gene="yodC"
                     /locus_tag="ACHKAU_001192"
     CDS             complement(52015..52197)
                     /gene="yodC"
                     /locus_tag="ACHKAU_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO5530299.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(52276..53193)
                     /gene="yedI"
                     /locus_tag="ACHKAU_001193"
     CDS             complement(52276..53193)
                     /gene="yedI"
                     /locus_tag="ACHKAU_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO5530300.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            53366..54286
                     /gene="yedA"
                     /locus_tag="ACHKAU_001194"
     CDS             53366..54286
                     /gene="yedA"
                     /locus_tag="ACHKAU_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO5530301.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(54275..54745)
                     /gene="vsr"
                     /locus_tag="ACHKAU_001195"
     CDS             complement(54275..54745)
                     /gene="vsr"
                     /locus_tag="ACHKAU_001195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO5530302.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(54726..56144)
                     /gene="dcm"
                     /locus_tag="ACHKAU_001196"
     CDS             complement(54726..56144)
                     /gene="dcm"
                     /locus_tag="ACHKAU_001196"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO5530303.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(56211..>56647)
                     /locus_tag="ACHKAU_001197"
     CDS             complement(56211..>56647)
                     /locus_tag="ACHKAU_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFO5530304.1"
                     /translation="EFVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEA
                     LGAIGLARVFAVSGALGLALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTAR
                     GKQLAQHNAQFLVEFMAKLGAELAGENEGIDHKVIDAFSPAG"
BASE COUNT        14132 a        14751 c        14198 g        13566 t
ORIGIN      
        1 ctgggaattt atctgtatac cgatgtagcg ttacctgacg tcgtggcgat gaccgggact
       61 ggtaacccgt tcctgatgtc gttcttcacc cagaccacag acggcaaact gaatctgatg
      121 catcacaaga aagcaggaaa cacaaagttg ggcgagttcg ggaattacag taacgactgg
      181 cagacgctgg agctggtgtt caccgccagc agtgccacgg ttactccgaa actgaatgga
      241 gtggctggcc cggcattcca ggtcataaaa gacagtctga cactggggct gaatgcactg
      301 acgctgacgg atattaccaa aaatgcaacg tatggcgttg agatagaaag tctggtgctg
      361 gaaataaata gtcctgcagc gtaaggatga acaggagagc aaaacagatg cttaagacaa
      421 acagcctccg gaagggcatg attcatgggt gtcgctggtg tcaggccaac ccggaaaaat
      481 tcaccatttt cgtggagagc ggcaacattg aaacgaccgg agaaacgccc tcgtttgttt
      541 accgctatca gatggtgatg tttgtcatgg attacgccgg ggagctggac gacctcacgc
      601 tgccgctact ggcgtggtta tccgaaaatc agccgcaatt gttgcttaat ccggagcgta
      661 atcaggacat caaattctcc gccgttatca atgacgatga cagcgccgat ctcctgttta
      721 cgctccctct gcgggaacgc gttcgcatca cgcgcagcag tcagggggca ccgcaggcag
      781 aacacctgca ggagccaaaa ccccgtctgc catcttccga aggcgactgg tcgcatgtat
      841 tccaggatgt gacgtggggt gaaagcgatg gataaggcat tcacccgcgt ggatgaaacc
      901 tttgaggcta tccgcgacag cctgaatcag caggccatca ataacatcgc cagaaagctg
      961 gcacaggatt tacgccgcgc ccagcaggcg cgtatccggt cacagaaagc gccggacggg
     1021 accgcgtgga caccacgcag acgccgcgta acccggatac aggagcgcat tcgctttatc
     1081 tggaataacg aagcacgcac gctgaaaaac tggcatcacg acacggggaa atacgggcga
     1141 accattaccg ggtgggatga ggataaaaac aatatccgca cgttttaccg ggatgacatc
     1201 gaccgctttc tggaaatacg cacccggcgc atcaaccagg acagcacaaa gcgcgtcccc
     1261 atgttcgtaa aactgcgcac cgcccgctac ctgaaagccc gtgcagatgc ttccggtgtg
     1321 acggtgggtt acagcggcgt ggccgcacgt attgcccgcg ttcatcagtt cggtgagcgc
     1381 gatcaggttg cgccgggcat tttcaccgat tacccggtac gtgagctgtt gggcatcagt
     1441 caggcagatg aacgcctgat ttataacacg gtgctgggcc ggattgcgga ggctgtacgg
     1501 tgagcgcaga actcatgcga ctgctgagca acatcatccg cactgggatc atctctgaag
     1561 ttgatgagaa gtcctggtgc gtgcgcgttc gcagcggcga actggaaaca ggctggttgc
     1621 gctggaacac cacgcgcgcg ggagccttca atgtgtggct gccgccatca cccggcgaac
     1681 aggtggtaat tgcctgcatt ggcggcaacc cggaaaccgc catgataatt ggcagcctgt
     1741 ggagtgatgc caatccggcc cccggcaaaa gcctgaaaga aatcgtggtc agcgcgccgg
     1801 atggcgcggt gttccgctac gacgcggacg caggcgcact gagcgccagc ggcatgaaaa
     1861 cggccaccct gcaggcatcc gtcagtgtga cactggacac gcccgtcgtg gaatgcacag
     1921 accttctgag aacggcgacg cttgacgtca caaaaggggg aaagatgagc ggcaatatca
     1981 cgcacagcgg cggcaatttc acctcaaacg gcatcacagt gcatacgcat aaacacggtg
     2041 gcgttaaagg tggcagcgat tcgacaggag gcccgcagtg acaacccgct acacaggaat
     2101 gaacccggac ggaacgggaa acctgaacga tatggagcat ctgaaacagt cagtcaggga
     2161 catcctgacc accccgctgg caagccgggt tatgcgacgg gaatatggca gccttgtgcc
     2221 cgatttaatt gacgaaccca tgaataacac cactcgtctg caatgcatga gtgctgccgt
     2281 gattgcgctg acacgatggg aaccccgcat tgccctggac gccatcgacg ttgtctggaa
     2341 ggcaggaggc cgcgccgggg tgacgctgtc gggcactgtc atgcagacca tgcagaatgt
     2401 tgaattaacc atcacgctga gggagtaaat catgcctgcc gttgaccttt cccagttacc
     2461 ggaacccgcc atcatcgcgg agcctgactt tgaggcaatt ctggctgaca caaaggccat
     2521 gatgattgcg tcctatcctg ccgaacagcg tgaagccgtc tccgccgcgc tggagctgga
     2581 atcggaaccc ctgaacgtta tcgcccagac aacagcgttt cgtgaaatgc tgttacgcca
     2641 gcgggtcaat gagggggcac gcgcctgcat gttaagccac agcgccggga cagacctgga
     2701 caacctcgcg ggcaatatga acacaaagcg cctgaccatc actccggcaa cggataccac
     2761 cgacgcagtg atggaaagtg acacctcgct gagactgcgg gcgcagcggg cgtacgatgg
     2821 cctgagtgtt gctggcccgt caggtgcata cgagtatttt gcccgcagcg ccagcggtct
     2881 ggtgcgtgat gcgcgggcta tcagtccgtc tccggcaaat gtgacggttt ccatcctgtc
     2941 cactgaaggc gacggcacag caacggaggc gttgcttaat accgttcgcg ccgttctgaa
     3001 tgcagaggat acccgcccgg tggccgaccg cctgacggta cagagcgcca gaatcgtgac
     3061 atggcggctg aatgcaaaac tgtactttta ccccggcccg gaatccgaac ctattctggc
     3121 ggcggctgaa tcgtcgttca ggaagtggct ggctgagcag gggcttatcg gtcaggacgt
     3181 ggcgttgtcc gccattgctg ccgcactgca tgtgcacggt gtgcaacgcg tggagataat
     3241 cgaacccaca cagaatatgg ccatcagcga catacaggcg gcgcgctgtg agtcgttcac
     3301 catcagcgaa ggtgggcgca atgagtaatt cactgttacc accatcagcc agcagtttca
     3361 tgcgttgtgc cgaagctgtc ggaacgcgca ttacagacat cccggtagac ctcaacacgc
     3421 tgtggtcgcc ggacacctgc ccggtgcacc tgctgcctta tctcgcctgg gcattttccg
     3481 ttgaccgctg ggatcgcaac tggccggaag agacaaaacg acaggtgatt cgtgatgcat
     3541 ggctgataca ccgacacaaa gggaccatca gcgcactgcg cagggccatt gagccgctgg
     3601 gatacctcat tcgcgtgtct gagtggtggg agttcggcgg agaaccggga acatttaccg
     3661 ttgaagtcgg cacactggac agtggcgtga cggaggaaat gtatctggaa atggagcggt
     3721 tgattgctga tgcccgtccg gtcagccgcc acatgacagg gctgaatatc attcaggaaa
     3781 ttccggggga tatttttgca gcggcggcaa ctcatgacgg tgaagttatt accatttatc
     3841 cggacgatta agcatgagta ccacaacacg aaaatttaaa accgttatca ccgatacagg
     3901 tgccaaaaaa ttagctcagg cagccgcgcc agatggtaag cctgtccgcc tgactcatat
     3961 ggccgtgggc gacggtggcg gcgcgttgcc cacaccagac agtaagcaga cccgtctggt
     4021 gcatgaggtg tggcgacaca ctgttaatcg cgtcatcctg gacgcaacac atcagaaccg
     4081 cattattgcg gagctggtta ttcctccaga aacgggcgga ttctggatcc gggaaattgg
     4141 tgtgtttgat gagcacggcg atttaatcgc ggtgggcaat actgccgaaa gttacaagcc
     4201 agccgttgcc gaagggtccg gacgtgcaca aacatttcgc accattctga ccgtatccag
     4261 cactgccact gtggcgctta ccgtggataa caccatggtg atggccacag tggattacgt
     4321 ggataacgaa ctgaaagagc atgaacagtc acgatgtcac ccggatgcct cgctgaccgc
     4381 aaaaggcttt gttcaactca gtagcgccac taacagcgat tctgaaacgc tggctgcaac
     4441 gccgaaagcg gttaaggtcg cgtatgatct tgctaacgga aaatatacag cgcaggatgc
     4501 cacaacagcg cgaaaaggcc ttgtccagct tagtagtgca accaacagta catctgaaac
     4561 gctggcggca acatcaaatg cagtaaaagc tgcctatgac aatgctgaaa aacgtctgca
     4621 gaaagctaag aatggtgagg atatctctga taaagacacc tttacgaaaa atatcggtgc
     4681 ctgccgtgca tatagtgcag agctgaatat tggtggagat agtgaagcat ggacaactgc
     4741 gcagttgatt ttttggctag agagtcaggg ggcatttaac catccttact ggatgtgcaa
     4801 aggctcatgg gcttatgcaa ataataaggt cattacagat acaggttgcg gaagtatttg
     4861 tcttgcaggt gctgttgtgg aagttattgg cacccgcggc gcaatgacca tacgcattac
     4921 cacaccgagt acatccagcg gtgaaggcat ccctaatgct caatttactt atattaatca
     4981 tggtgatgct tatgctcctg gctggcgaag ggactataac tccaggaata agccaacagc
     5041 atcagagatc ggggcgttac cgtcagatgg gacagcagta tcgtcagtta atctggcttc
     5101 aaaaggtcgg ctgaccgccc tgacagataa tatgcagggg gccacaggtc tggagttata
     5161 cgaggcgtat aacaacggat atccaacaac gtatggaaat atcattcacc tgaaagggat
     5221 gacagccgtt ggcgaaggcg aattactcat cggctggagt ggtataagcg gtgctcatgc
     5281 tccggcattt attcgttcac gacgggatac gcccgacgca aactggtcgc cgtgggcgca
     5341 gctttacacc tcggctcatc ctcctgaaga gttttatcca gtcggtgcac cgattccgtg
     5401 gccatcagat accgttccgt ctggttatgc cctgatgcag gggcagactt ttgacaaatc
     5461 tgcatacccg aaacttgcag tcgcttatcc gtcaggcgtt attcctgata tgcgtggctg
     5521 gacgattaag ggcaagcccg gcagtggtcg tgccgtattg tctcaggaac aggacggcat
     5581 taaatcgcac acccacagcg ccagcgcatc cagtacggat ttggggacga aaaccacatc
     5641 gtcgtttgat tacggtacta aaacgaccag ttcatttgat tacggcacaa aaactacgaa
     5701 tagcgctgga aatcattcac acaatatacc tgttggtcac actggcgcgg ggaatggtgt
     5761 atcagccggt tataacgctg cgttaggtac tggtaccacg tcgagcgcag gcgagcatgc
     5821 tcacaatgta tatatcggtg cccataacca cactatcggc attggtgctc atgcccattc
     5881 tgtcattatt ggtccccacg gacacaccat caccgttaac gctacgggta acgaagaaaa
     5941 caccgtaaaa aacatcgcat ttaactatat tgtgaggctt gcataatgac attcagaatg
     6001 agtgaacact cacggaccat aaaaatttat aatctactga ccggaaccaa tgagtttatt
     6061 ggtgaaggtg atgcatacat tccacctcat acaggtctgc ctgcaaacag taccgatatt
     6121 gccccgccag atattccggc tggcttcgtg gccgttttca acagtgatga ggcatcgtgg
     6181 catctcgttg aagaccatcg gggtaaaacg gtttatgacg tggcatcagg ggactcgtta
     6241 tttatttctg aactcggtcc attaccggaa aatgttacct ggttgtcgcc gtatggagag
     6301 tatcagaagt ggaacggcac atcctgggtg aaagatgcag aagcagaaaa actgtttcgg
     6361 ataagggagg cggaagaaac aaaaaacagc ctgatgcagg tagccagcga gcatattgcg
     6421 ccacttcagg atgccgtaga tttggatatt gcaacggagg aagaggcatc gttactggct
     6481 gcatggaaga cataccgggt attgttgaat cgtgttaata cagcggtagc agcggatgtt
     6541 gagtggccag tcgccccaca ataaagagaa aaagccatcg attgaaatat agatggcttt
     6601 atgtactcta tttatacaat acaacaccgc tctttttagt tatatatgtg cagttcgatg
     6661 gtatatcttt atttataaaa gacattgcac ctatttttac attatcccca attttacgtg
     6721 ataatccaat gatgcaacaa ttagctccga tatcaacgtt actaccaatt tttactcttg
     6781 aaccaggcat gtcaccatct atctgtccaa tggtagtatt ctgtcgtaac accagatttt
     6841 caccagcatc aacagcaaaa tgaacaacaa ttccagcatg atggggaatt gttaaccctt
     6901 ttccaatatt tgcgcccaat ccaatttcac aaccaaattt gttaattatt ttactgttta
     6961 actttttggc tgctttctta tgtaatttat taccattaat atacatttcg ttagccaacc
     7021 gccaccagaa aaggaaattc cggttacgct gttttttctc tcttaaaagc ctccagatat
     7081 ccatacgttt ccgccgaatt acttcatatt tccagaagtt ttttaaatta gtagagtccc
     7141 caaataaaac aaagtgaatt gccattaagt aagacagcac gataatctcc ttaattatta
     7201 tttcagacca cacatattat aaggttaaga gattataaaa tcctgttgtt tgttattcaa
     7261 aaacaatttt ctgagaagga catacaacag caagtcgcca gtcaccttca tcaggaaatt
     7321 ggcgacatac gttaaatcag agcagcccct taactgagct ggtcgcgcta ttaagggatg
     7381 atgtcacctt atctttgaag ccggacaaca tatcgctgaa cgatgaggat tgcaggcgct
     7441 cccgcaaatc ctcatcacag cgttcaagag tcagtgaaaa ttctatcttt ttcgacttac
     7501 cgtagcgatc aaactcggag cgggtcgtat tcgtttcggt caggacatac atgccgtaaa
     7561 tctgcccgac gccatcaatc agaggccagg gccgtcctgt atacgcctgc gtggtcagca
     7621 gagacagcga cacttcgcca cctgtaattt caggataaag cacaccagaa agaacgatgc
     7681 gatcatcacc tgcaccgata tactgccagc ttgctgaacg gttaacgcgt tcatttttca
     7741 catgccgcca gcttttattt tgctgtaact gctgatgcgg cagcgtgcgc agctcaaaaa
     7801 caaacatgcc atagatcatc atcatggcca tgactcctca atctttatcg taaaaactgc
     7861 cacgcccggc acgggcgcgc cgttccattt ctgccctgac catttcaccg accagtttcg
     7921 ccagttcgcg gggattctgc gtaacaacgt tatgcagatg aacatgaatt tcaccgccaa
     7981 atctggagac agcaggctcc cgattacggg aagctgcagg aactgatgcc actggcgatc
     8041 gtatggcctc cgccaccggg cgggagctgg ccgcaacaac agggaccagc gccggaggca
     8101 gcggagccgg gactacgggt gtgatattaa ttgcgggggc aggcttactg acctgcgcaa
     8161 tcttccgctc ctgccactcc ccacgaacag caagtgcgcg gggcaggttc ttaaagacaa
     8221 tatcgccagg gccaatgcgt tttttcgtct catcaaccag cttacctgtg ttatcagcaa
     8281 ttttgctgag tctgcgtagc gtcccggtat tgctgtctgt gagcggtttg ttgtctttgg
     8341 gtttatcacc tccggtgcca ttgccatttt ccacaggctt cggcggattg attttcgcca
     8401 ggtccccctg aagtaaggca accttatcct gaagaatggc cgcacgctgt gcgtcttcga
     8461 ttttcttgcg cgccctttcc gcttcatccg gaaggacgcc aagtttttca agtatccacg
     8521 ccagcgtatc cagtagcatt tttgcaggtg tcagaacaag ttgtaacgca ccgccaagaa
     8581 cgttaccgaa tatctcgcca gcactggtac atttatccaa cgtttccttg ctggactcca
     8641 tcggtgacag cagcgattta aaccagttaa acacctggct gatcccgctt ccgattgcgt
     8701 caaaaacagg gccaaaccgt tcaaaggttt cacgcaacgg gttcagcctt tccataatcc
     8761 cgctgaacac cccggcaaaa aatgccctga tgggatccca gtatttccag ataaggacgg
     8821 cagctccggc aagcgcagcc acgataagac caaccggact gaacagcgcc ccgatagcgc
     8881 ctcccagtaa agaaacggaa cccgtcacca ttccccatag tgctggcagg accctgacag
     8941 cattcattga tccggtcagg agagaaaaac caagacgcag ttttgccagc ggaccagcaa
     9001 gcacaccaat agccagcgac aacgagccaa ccgttgcagt cattgccagc aacgcaccgc
     9061 ctgctatcag tagctggcgc gtcagtgccg gatgggcctg cgccagcgcc gtcacccttg
     9121 ataccacccg cgtgagccac tgcgtgacag aacgcagcgg accgtcaatc agatctgcaa
     9181 tgcggatgcg caacccttcc catgcactgc cgagtgattt cagatcgccg tcaaggttgt
     9241 tggccataac ctttgctgtg cgttcagcct caccgcgcgc gccttcaagt tcttttctca
     9301 gtttgggtaa ggaaccgtca cccactgcat caacgagcgc cataaacgat gtgaaagcct
     9361 cttctccggc aatgtcctta aagaacgata cccggtcaac ttccccgtat ttgcgggtgg
     9421 ctttataaag gtcggccagc acatcctcca tcgggcgcat tttacccccg gcatccgaga
     9481 cggacacgcc cagctctttc agagcttctg ccgccgcctt tggcggtgat gccagacgag
     9541 ccaggctggc acgcattgcc gtcccggcat cactccctct gatgcccata ttcgccagca
     9601 caccagccat cgctgcggcc tgctccagcg atattcccag cttacccgcc accggacctg
     9661 catatttcat ggtttcgccc agtgcgcgaa ggtcagtgtt ggtacgggta aacgctgcgg
     9721 tgagtgtgtc accgacccgg tccatctggt cagcagaaag gccgaactgc gtcaggatat
     9781 ttgagccaat atctgccgtc tcgccgagat ccataccgcc agccgttgcc atgctcagca
     9841 ctccgggaag cgcagcctga atggcctgcg gtgtgaagcc tgccattgca agaaatgcct
     9901 gcccactggc ggcatcgcct gcggtgaact gcgtttcaga gccaagtttt aacgcctgct
     9961 cacgcagcgc cttaaactgc gggctgttct ggtcgattcg cgtcagcgcc tgaacgcggg
    10021 acatctcttt gccgaacccg atcgcgggct gcaaaaaacg cccggcagca tagccgcccg
    10081 ccgctgccgc accaattgcc agcgcaccac ctgttttcag ttttcccgcg gtttcctgcg
    10141 cgcgcgaata ccacttacgc gcccgcgtta cacgcgcaag cgcctgccgt tcgcgttcaa
    10201 gctggttgtt gtactgttcg gtgcgtctga tggcctgctg gatggtgtta tcgctgcctg
    10261 tcagggaaat gccgtggcgt ttcagctctc cgccaagctc ccgcattttc tgaatttccc
    10321 gtgtgcgcga ttcattcagg cgttcaagcc gggtgcttaa ctgctgcatc agcttttgtt
    10381 gtttttcgct gagcactgta cccgtgcgtt gtaactgatt aagggcgtta agctggcgtc
    10441 gtgctttcac gatacccgca tccgctttac tgacagcgtc gcgggcgcgc tcaaatgaac
    10501 gagcctgacg ctcgagattt ttaatcgccc cctgcgttcg ctggatggag tcaccaaact
    10561 gccccatcag gcggcgggcg ttttcggcag gccgggtcag cctgtcaacg gcgctgaaag
    10621 cgacccggat atcaagagtc ttcattgtct gcattcccgc tgcgaagtgc cgcccgctca
    10681 cgccagctaa ccacttcgcc gggcgtcatc atgaagattt cggcgggcga ccagttaaaa
    10741 ataacggcaa tatctgccac aaagtcttct atgtgctcaa agcacacaac cgtgatcagg
    10801 cttccgtcgc ctgttcgttc ttcccgccag agtccgcacc gctcaaaaaa tttacggcaa
    10861 ccacacataa ctgaataaag tcacgggatg ccattttttt gatcgtcact tcatccagtc
    10921 gcggtgatgt cacgcgtgac agcagcgtaa acatggattc cgctttcaga ttcagcacat
    10981 cagacagcga caaatctcgc agagatccag cctgctcaat agccccggtg atctccacat
    11041 acgtgatttt ttcgccgcct cgctcaattg gttgggtaag ttttacacca cgctcactgg
    11101 tttctttcac agtgtcagca actaccgtgt tttcggtatc gatgtttttc gtctctttca
    11161 tcaggaaact cctttcagtc agaggcgacg cactgcgccg cctgcatatt acttatcagc
    11221 caagcccgag cgcggaacgg atgcgatcgg gcacaatgtc cttgccgtcc ttccggtaaa
    11281 tgaagttcag caggtcaatc tcccacaacg ggcgatcgtt aacactcagc ttgtagtagg
    11341 tgtttttaat ggcgtaagtg tgtgatgtgg cttcgccctg tttggcttcc cccatatcaa
    11401 tttccgtcac acgtccgcgc atttcgactt catacaggtc gctttctgca tcggtgtaat
    11461 attcacccgc aaaacgcagc agcgtgccgt caatcgtgcc gccatactta aggaacagct
    11521 cacgaactgc gccccccatg acaaagctcg catcaagcgc ggagtcgtcc agaccgagat
    11581 caatacttac cgcacccatc atgccaccac cccggtagct gtcggttttg cgcgtcagct
    11641 taggcagagt gacggacgtc accttaccca cttcgttttc accatccaca aacagcgtaa
    11701 aaaagcgaag atgttttggt acagccatca ggcacctccc agcaccgcaa atgcgggttc
    11761 aaagtattca tcagtaaacg tctggtaaag ctccatgtct tccagtggcg gaacgggcgt
    11821 atatttgtag cgaatacgca cacgcccctg acgtaaatcc gtggtgctgt tatccaccac
    11881 gtcataccag cactccgcgc caatcagttt cccggcagta accagcgaat ccagttttgc
    11941 cctgatggca ctgataacat ccttcacgtt cgcaggcgtc agtggactgt cgatggtttc
    12001 aaactgcgct tccgcaattg aatcagccag cacctgtgcg gttcgggtat acacctcaaa
    12061 gatgtaggcg ttcgtttccg gtgtgcggtt gccccagaag cggaacccgt tgcgacgaat
    12121 aatggtcgtg atttctttgt tgttgaggct gttggcatca ctgtcttcgg cctgcaacga
    12181 ccagaaaaca tgcctggaca tccccagcac atttttaaca ggaacgttgg acagcgattt
    12241 gtgccagccc tgctcatggt caatgtacgc acgaaggccg cacgcatagg caggcgcggg
    12301 gaacgtttcg tttttgccac ttttcgggtt gtaggcgatg aagtccggcc ataagagcat
    12361 cacctcacgt tcgttgaatt tctggcggta ggtaattgcc tcagccatcg tgttacagtc
    12421 atgacatgag gcatacacaa acgcgcgcag tttacccgca atcacgcaca gggattttgt
    12481 caccgcctcc gtgtccagct ccggcgcggc cagaatacgc ggacggtatc cgatgctttc
    12541 atcctgctct gcaacaagca gcgcatacat ccccgtatag ctgccgtcag attcagaacc
    12601 accgataacc agttgatcct gcgtcttacc gccttcttct ttgtgttcag ccacgcgaac
    12661 gacgatcact tttgtgctca cctggtctgc aatagcctta agcgcacgat aaagcgtccc
    12721 cgtagtcccg cattttccca gcacgtcatt gacgcgggtc agcagtgtgg gcttgttcag
    12781 cgggaacagc tccgcgtccg catcatccgc cgttgccacg ataccgataa cactggaatc
    12841 aacatcatta atcgctgtta ccaggtcggt actttccgta acacgggcac catgaaaacg
    12901 agtttcactc atagcttcag ccccttgtat ccgttaaatg attcggcaac aatcatcacc
    12961 caccacgcgc gtaatctcac ccctgcgccg ttctcccgcc acggcgacaa caaaaagcag
    13021 taacccccta tgcacgcaca tgcgaccatg ccgcacaggg agggaacaga tgaccgatac
    13081 caccatgcaa ttgctcagtc agagcacaga ccccgtgaaa atgccggatt ttgatattct
    13141 cgcggagggg gaaacactgt ccggcgtggc agagcgcctg atgagcctgt cactgaccga
    13201 caaccgggga tttgaagcgg acctgctcac catcacgctg gatgatgcgg atggtcagtt
    13261 gcagctaccg ccacggggcg cgcgcctgac ggttctcatt ggctggaaag gagaaccgct
    13321 gacagaaaaa ggcacttaca ttgttgatga aatcgctcac gaaggaccgc cggacaggct
    13381 gactgtttca gccagaagcg cagattttcg ggatgaattt aacgttaaac gtgaggtgtc
    13441 ctggcatgat gtgaccgttg agcgcgtggt atccgccatc gctcatcggt acggtctgaa
    13501 accacaaatc agcgaaatgc tgatggatat tgaaatcgac cacgccgacc agaccgaaga
    13561 aagcgacatg tccttcctta cgcgcatggc ggaaatgctg ggcgcaatca ctacggtaaa
    13621 aagcagtaat ctgttattca tcatgccagg cggtggcgtg aacgcacagg gccagccgtt
    13681 gccatcgttc gccatcacgc gcagcagtgg cgatcgccat cagttccgca ttgctgaccg
    13741 cgaagcgtat acgggggtac gtgcttactg gcttgatctt aattacggga aaaagaaaaa
    13801 agtcagcgtg aaacgccgca aaccgaaaaa ggagaaaagc agcagccgtg aaggtgacta
    13861 tatggaaggt gcggaaggca acgtgtttgt gttacgcaag acttatcaga acgagcaggc
    13921 agcaagacgt gcagcggcgg caaagtggca gcagctacaa cgcggagccg catcattctc
    13981 catcacgctg gcgcgtggac gcgcagaact ctatcccgaa atgcatggca cggtgacagg
    14041 cttcaaaagt gacatcgata atcaggactg gattattgca aaagccgagc acaccattga
    14101 taacagcggc tttaccacac agcttgagct tgaagcaaaa atcccggaat ggatagcaga
    14161 aacagaatga gcaaattaga tgtattgact caaaccagaa ctgacacact cacagcacag
    14221 tgccagaccc aatctggcag cccgctttgt gcctagagca ggtgttgctt atgtaacagt
    14281 taatccacgg atagtggtca tgaatgctgg ttgaagttgt attgccatga gtggtcaatt
    14341 agggactcac ttattatcgc gtgctcacca ttgatgggta agatcatgca tttcggctac
    14401 tgtacttcgc gaatcctccg cacacaggca acacctgatt taagaacaga gggagatgat
    14461 gacgcaagga agatttatta tcgtgcatgt ggcagatgat cttttagctg ccggagtgat
    14521 aaattacacc tattttcgat aagtgttttt gttatagaac attaatttcg tgggaaacaa
    14581 taaagtgaaa ttaggaagaa atgataagtg ctggtgtaat tcaggactta agtataaaaa
    14641 gtgccatctt ggtcgagatc gtgaaactcc ggtatccaaa gccgaggcac tgaaattttc
    14701 aaagtctctc tcgcagagaa aggcatgtta tgcgccagca tccatgcacg aagattgtga
    14761 taaacgcatc gttcaggcgc atacgatatc taagagcagt agccttaagg cgatcgccga
    14821 ctccagcaat cacgtcatgg gggtgataat gaacttagca agcctgatga gaaacaacgg
    14881 aaaatggctt cccgagaaga tcggtatcaa tcaggcatcc acttttacag gattttgtgc
    14941 agttcatgac agaatcttat tttcatgcct agagaacgaa aaatttacag gcacggacga
    15001 gcagtgcttt gccttaatgt ttcgttcatt gtctaaagaa atttatgcca aagaaggggg
    15061 agtgctatct tctgattttg ctaaaaatgc cgataaagga aagctacaag ttgaacagta
    15121 ttatattcag aaattaatcg accagcacca aagtggctta aatgcagcaa taattgaatt
    15181 aaataatctt aaaactagcc tcgaccgcat cctacttagc aggcaatttt cagaaattga
    15241 aagtgttatc ttcacttttg cagagccttt accaattgta gtttcgtcaa ttctttcacc
    15301 ggagagggat ttcgatggga ccaaaattca ggatttgagc gatcttacag tgtcggcgga
    15361 acaagtatgt ttcaatgctt tttcctgcgg aggtaaggga tatgtagtgt tcagttggct
    15421 gagaacttca acaattatca gacgttttat acggacacta attaagatcc cagctaacag
    15481 gatgttcaat acccttctgt attttttctt cacaaaggcc gaaaatacat attcatcccc
    15541 tgagtggtgg gacaggctca gtgataaaca gcgagagaat atcggcaaca tgattatgtc
    15601 aggggttgaa ttttttggca gacctacatc acaggttgat cattccgttg attataacgc
    15661 cgctgcactt gaagagataa catgcagcaa caacgaaatt tgcagctgaa tgtttacatg
    15721 ctgttagaaa aagcgggctg aacaatcatt tcgggattgt aacagataag atggcactgc
    15781 ccgcttgctt cccgatatgc tcagccaata aacttatgta agcaaccgat ttccatttat
    15841 gcggaggtta tcaaagttgt tggagttgcg aacttccgct cttcgcacaa agcgtaccgc
    15901 taggtttgat tgtattctgg ctgcgcaaac tgtcagttgg agtctgagct attacaactt
    15961 agaatagcgg cagcaccacg ttaagggagg tcgctatgtt ccgttgtccg ctttgtggcg
    16021 catctgcccg aatccgcacc agtcgtccgg aaaacgattc aaacactgtg cggcaaaagt
    16081 attaccagtg taacaacctg gaatgcggcg tatgcttctc aacactggaa gctttccata
    16141 aattcacatc aaaacacgcc tccggcgttc actcttcgga aggtatcccg tggcatgagc
    16201 tgccagcttc acacagggga aacaatcaga tgagtttgcc tttacctcag aattaacagg
    16261 cagaattgcc ggagtaacaa aaaaacgata gattacccgc gggtgccttt cggctgatgg
    16321 tcggaggaat acccgaaggc cggatgtgga aaggccccgg aaaacatttc tgtttaaccg
    16381 aggccctaac atatctacct taagcaagtg ataggttagc gcctctccaa caaaggagca
    16441 agcgctatgt cgcaaaaatc gcttacagcc atcacgttct gcgtgacggt aatcctcatc
    16501 atctggatgc tacgcggttc gctgtgtgaa atacggatga gcttctgggg agcggagttt
    16561 gcggcgttct tacagtgtaa gcagtaagga aaccgcgacg ggggagcaat cccccgtcaa
    16621 tcggttgcta aggttggctg aagtggcacc ctatctcaca gacatgaaca acaaacccgc
    16681 agcgtaagaa ctgcgggttt tctttttgga acaatcacta gctagtgtac cgttaacggc
    16741 acataatgct cgcacaacaa acctgttaaa agagattact cagcattctc ctgcttccat
    16801 tgccggatca tttcatcggt aacatcaccc tcataacata ccacgtcata ccctccatta
    16861 cggctatatg cactgcgtcc accacatcta ctaccattcc ttgcatgatt ataaggacac
    16921 gcacaattgc ctggataaga ttcaatggat tctttaatta tttctttttt gatctgagca
    16981 tcagactttc ctgttgctgc atatccacta aaagatacta aacaaaggca gattgccatt
    17041 aataaaccac atcgcatacc gatacactct attactaacc aatcatagcc cacatcataa
    17101 aatcgagatg aggagtacaa tacctttaac aagaaggtgt cataacgata atatgtcgtg
    17161 caattcatca aaaaaaagat ctatatcaaa gagatagaac tcttatcagt gcttcagaca
    17221 tcggcattcg aggcagcaga aaaacagtaa atctgacgaa tttgcctttt cccaagatgt
    17281 tgtcagatta aaaagataga ttagcgcagg tacctgttgg tggattgcag gaggaaaaat
    17341 gaagtcgagt gaaaaaatcc cagaaaaatt tttgtttaac agaggtcctg gttcactatg
    17401 cgaaatacgg aggagctgca gaagaattct gcttcatcct tagagtttaa acagcacgtg
    17461 cactaccatt gtttctgcat atcgccacac catcgccact ctaccgccat tgcacaaaat
    17521 acaaatacga aaaaaccacc cgaaggtggt ttcacgacac tgcttattgc tttgatttta
    17581 ttcttatctt tcccatggta cccggagcgg gacttgaacc cgcacagcgc gaacgccgag
    17641 ggattttaaa tcccttgtgt ctaccgattc caccatccgg gctcgggaag aaagtggagg
    17701 cgcgttccgg agtcgaaccg gactagacgg atttgcaatc cgctacataa ccgctttgtt
    17761 aacgcgccaa attcttcagg cctttcagcc agacatccgc ttgacgccga tgtcttttaa
    17821 actggagcgg gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac
    17881 caactgagct attcccgcat tcatcaagca atcagttaat cacttgattt tattatcgtc
    17941 tggcaatcag tgccgccgtt cgatgcgttg cattctactt acctggcgcg atgagtcaac
    18001 gatatttttc accacttttg atcgtttgct gaaaattacg ccgaaacgat cactgatcaa
    18061 gcaaatctgc acgcgcagcg ctcaaatatt gcaacattga ccacagagtc agtaccgcag
    18121 ccacaaagaa aagtgcaata ccggcgtact caacccaaat gttcggacgc cacagcagcc
    18181 aggccaacgc caccatctgg gcagtggttt tcactttccc aatccaggag acagccacgc
    18241 tactgcgttt acccaactcc gccatccatt cgcgtagcgc agaaataata atttcacggg
    18301 cgatcatcgt tgccgccggt agggtcaccc accagctgtg ataatgctcg gttaccagca
    18361 ccatggcgat agccacgaga actttatctg ctacagggtc aaggaaagca ccaaaccggg
    18421 tactctggtt ccagcggcgt gccagaaaac catcgaacca gtcagtcacc gccgcgacgc
    18481 agaaaatgag cgcggcggca aacggcgacc aggtgacagg cagataaaag accaatacaa
    18541 agaatgggat aaggatgaca cggaacagtg taagcaacgt agggatatta aattgcataa
    18601 tgacgggtaa ctatctgttg tcagtaagat tacccctatg ttgctacaga gacatcaatg
    18661 tttcaacgac cagaagatct tttctgccag accttgcgaa atacccggca cttttgcaat
    18721 ttcctcgacg ctggcgttac gtaaaccttg caaaccgccc atatatttca acaacatttg
    18781 ccgacgtttt ggcccgacgc cttcaatggt ttccagggag ctggtatttt tgaccttcgc
    18841 ccgtttttta cggtgcccgc caatcgcgtg atcgtgtgat tcatcgcgaa tatgctggat
    18901 aacatgcagc gcgggagaat ctggcggcaa actgaatccc tcaccttccg gctcaaagaa
    18961 cagtgtttcc agcccagcct tacgatctac tcctttggca acgccaagta gcagtggatg
    19021 atttttatcc catgagacat ccagttcggc gaagacattt ttcgcctgcg caagctggcc
    19081 tttgccgccg tcgataagga tcacatccgg gatcttactg tcgtcaatgg ctttaccata
    19141 acgccgacgc agcacctgat tcatcgccgc ataatcatcg cccggcgtga tgcctgtaat
    19201 gttatagcgc cgatactccg cacgcagcgg gccgttagca tcaaacacca cacaggaagc
    19261 gacggtttgt tcgcccatgg tatggctgat gtcaaagcac tccatccgct tcacttccgg
    19321 caatttcaac acgctggcaa gcgcggtcag tcgctggtga acggtagatt gctgcgaaag
    19381 tttgctggtt aaggccgtcg ccgcattggt gcgcgcgagt ttcagataac gcgccctatc
    19441 gccgcgaggt ttggtttgaa cattaatctt gcgtcccgcc agttctgaaa gggaatcggc
    19501 gagcagcgtt ttatcgctaa gattaaaatc gagcaggatc tcacccggta aggtgcgcat
    19561 ctggctgcct tgtaaataga actggcctac gaaggtttct accacctcgc tcagttccgt
    19621 accgccaggc actttcggga aatagctgcg gctgccgagc actttgccct gacgaatgaa
    19681 caatacgtgg acacaagcca tgcccgcatc gaacgccaca ccaataacgt cgaggtcgtc
    19741 gccggtattg gaaacgaatt gtttttcggt gacgcgtcgc accgcctgaa tttggtcgcg
    19801 aatacgtgca gcttcttcaa actccagatt ctggctggca gtttccatac gactaatgag
    19861 ttgcgtaagc acctgatcat ctttgccaga caaaaacagg cgcacatact cgacctgctg
    19921 agcgtattct tcttcactca ccagtccttc aacgcacggt cccagacagc gccctatctg
    19981 gtattgcaga cacggacgcg agcgattgcg ataaacacta ttttcgcact ggcgaatggg
    20041 gaaaatcttt tgcagtagcg ccagtgtttc acgtacggca tagccattcg ggaacgggcc
    20101 gaaatattca cctttggcat gcttcgcacc acgatgcatc gccagacgcg ggtgggtatc
    20161 gccactcagg aagataaaag gataagattt atcatcgcgt agcaaaacgt tgtaacgcgg
    20221 ctgatagagt ttgatgtagt tgtgttccag caacagcgct tcggtttctg tatgagtaac
    20281 cgttacatca atttgctgga tctgggcgac cagcgcttcg gttttgcgcg aagcgaggtt
    20341 gctacggaaa tagctggaaa gccgtttttt caggtctttc gctttgccga catagataac
    20401 cgtaccacca gcatcgtaca tgcgataaac gcctggctgg ctggttacgg tttttaaaaa
    20461 cgcttttgcg tcaaactgat cactcactga cttgataatg tctccgcatt acacagacca
    20521 tggcgaattg ccaggtgagt cagctcaaca tcgccatgaa tgtttagttt actgaacata
    20581 cgatagcggt agctgttcac cgttttcgga ctgagattga gctgttctga gatctcattg
    20641 accttctggc ccttggtgat catcagcata atctgcaatt cacgttcaga caaactggca
    20701 aatgggcttt ctgttttttc tggttcgatc tggcttaacg ccatttgttg agcgatgtca
    20761 gaagcaatgt aacgctgccc tgaatagaca gaacgaatcg cactcacgac ttcctgcgga
    20821 gccgcgcctt tgctgaggta gcccgcagca ccggcctgca tgactttcgc tggtaaaggg
    20881 ttttctgtat ggacggtaag catgatgatt ttgacatcag ctgtggaacg cgcgatttta
    20941 cgcgtcgcct caagaccgcc aatgcccggc atactcatgt ccattagcac cacgtcaacg
    21001 gcatttgccc gacaccactt aacggcgtct tcaccgcacg atgcctcacc gacgactttt
    21061 atacccttta tatcttccag aatgcgtcgt atccctgcgc gcaccagttc gtggtcatca
    21121 acaagtagaa cgttgatcaa aggaatatct ccagaaatag ggataacgct actgatagtt
    21181 agtcattcgt atattagcgg tttttattgc aactttgaaa cgttaaaaat ggtgggtatt
    21241 cgatttttct ctcgtttttg tcaattcgat tgtccacagt tatggtcacg cccgtcacca
    21301 acggcttacc ggatcgtttc acagtaagcc ctgcgacgaa atgtgacaaa aattaccttt
    21361 attcagcaaa aatgaaaatc agcccgctga atatgtaaca ttaattaacc cgaacaatac
    21421 gggcaaaaaa cattcatgat aaaaatattt attggtcatt atattaacgc atttaatagc
    21481 accgttgacg atacgctcaa aaaacaacca ctgctatttt tagcaaagct tatggtatac
    21541 tccgccgcct taacattatt caccgcaaat tttcattgca acatgacgag gaaaataaat
    21601 gagtacgcct gatttttcta ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg
    21661 cctgaaagcg atgctgacgc tgatgctgca ggcgatggga caagctgacg cgggccgcgt
    21721 gatgttaaag atggaaaaac agcttgcgct gatcgaagac gaaacccagg ctgcagtatt
    21781 ttccaaaacg gttaagcaaa ttaaacaagc ctaccgtcag taatataaaa ccggctgata
    21841 gcgtgccttt cagccggttt ttgcatctgg cacgcaggac agaaaagaga tcaaattaag
    21901 ccagtagcgg ccgcgtaaca ggcaacctgg gtcttattcg gtgcattgat ttttttctgc
    21961 atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat cgctatctct
    22021 gctgatgtct tcccttccgc cgtccacctc agaatttctt tttcgcgctt gctgaaattc
    22081 atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc
    22141 accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgcgctg
    22201 caacgggaaa aggacaaaaa gcccagcgcc cggtttggca gcattaaata ctgagtgaca
    22261 ccgcggcgta aaccatgtgc gcgcgcggct tcccataacg gctgtgcttc gttgaataag
    22321 tcatcattcc acattagatg gccctgacta aagttttcag ggttcagcac cggatcaatt
    22381 gcgagaaagt tttttgcctg ataataacta acccacgcct cagggtaatt ggtgtagaaa
    22441 gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac
    22501 tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc ctccatcctc
    22561 tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac
    22621 cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa
    22681 gatttatata taatttatat tattcaggca atgaattact tttgcaagcc atcacattct
    22741 cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatagcg
    22801 tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca gcagaaactt
    22861 ctcgaggaac tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc
    22921 ctgctcgact atccgcccct ggtccataaa aatcgcccgg tccgcaacat cccgggcaaa
    22981 gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct gacggatggt
    23041 gttcaggact tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat
    23101 cacctcagga cgcattgcca gcgcacgtgc aatcgcaaca cgttgctgtt gaccgccaga
    23161 caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca acagctcgcg
    23221 agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat
    23281 gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg
    23341 ctgacgcaac tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt
    23401 gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctgcgtag
    23461 caacgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac
    23521 ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat tttttcacca agttcttaac
    23581 ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc
    23641 tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc caggtacatg
    23701 gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc
    23761 tggattgtcg cggccagcga cgtatccttt accaggctga taaagctgtt cgacagcggc
    23821 ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatagtctg ccatggcgtc
    23881 ataccaatac tggcagctgc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc
    23941 agtgtttcgg cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg
    24001 ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg
    24061 agcggtgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt
    24121 ggcgacaggc gcatcagcgc gagaataaac ccgagcagta agccaaaaaa catgccgccg
    24181 atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga atcaataacc
    24241 agttgtatac tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca
    24301 gcgcaggcgc accgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct
    24361 gactgatacc gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa
    24421 tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt
    24481 agccaggtgc aaaatagtca tcccagagta taatttccgc tgatgcggtc agctccagtt
    24541 ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ctgatcggca acggaacgcg
    24601 acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc
    24661 cggcgtgagt tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt
    24721 cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag
    24781 aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct
    24841 cttccagttg ggcattggga tcggtcagtg cttcgcacat ttcaatctgg gtattgaaca
    24901 gatccagcaa caagcgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat
    24961 tttccagcag cgccacacag tgcagaccga gtttcgccgc aactgcggca gtctgccgca
    25021 catggttaga ctggatcgcc ccggcagtaa tcagcgtatc ggcaccttcg cgcagagcat
    25081 ctgctgcgag aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacgt
    25141 catcccgttt gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga
    25201 gcggtgttgg cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca
    25261 taacagcctc cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa
    25321 taaaaaaggc gctggtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc
    25381 agcaccaaac catttttcgg aaagggcttg cagagtgcca tctttttgca tttccgcaat
    25441 tgcatcgttc actgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc
    25501 ctgacgggag aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc
    25561 cagcgccgcc agacggtcaa cgaggatcgc atcgatacgg cctacgcgca gatcctgata
    25621 tttggtcggg tcatcatcat aggtacgcac atcgacgccc tgaacattct gccgcagcca
    25681 ctcttcatag ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt
    25741 tttaatggtg ccttcgttac cttttttcac cagcgcctga ataccagaaa tggtgtacgg
    25801 ggtagagaaa tcgtattttt tcttgcgctc atcagaaatg gtgacctgat taatcaccac
    25861 atcaatgcgt ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga
    25921 cgcctcaacg ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa
    25981 tttgccgtca tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag
    26041 cgtgccgcgc tctttaactt tattaagcag accttcatct gcaaaacttt taacgctcat
    26101 gcccgcaacc agcgctacgg ccatcacact catcaatgcc tgacgtccca gatgtgctaa
    26161 tttcatattt accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa
    26221 cacaaaaaca actccgctac atcttattct tatttaatat atatcagaag caggcaggct
    26281 ggaggatttc tgcacaagtc cggtacacgt ttgacgctga tagtgttgat acttccgtaa
    26341 tgcgatgcgg taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag
    26401 gaaaccgtcc tgcagcggat cgagggaaat attttgctcg tgtagatgat tccccaaacg
    26461 gcgcaaacga acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag
    26521 aaattgctta aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc
    26581 gcaaaatcgg cacaatgccg cccatgattt ttgttccgtt tgccagccat tttcatcaag
    26641 cagcgcgtcc aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaacgagggt
    26701 aatgcgatcg agtaatttac ggcaatgcgc gcaatgagtc tggctgtgtt taaagtcttt
    26761 aagatagcgg cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta
    26821 gtcaaagtta aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg
    26881 tttaatagcc tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc
    26941 aatctctttg agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc
    27001 cggcaacgtt tcgatggctt ccatcacccg ctgacgcaga ttactgtcca gcagttgttg
    27061 tagcgggttt tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc
    27121 gcgccactca tcgtaggaga agagctggct gttattggtg tcgagcaaca tttggcgata
    27181 atcggcaata tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag
    27241 ttcctgctcc agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg
    27301 cggcacccag tcacggctgc gaagttcatc cagcatagcg ccacggatac gctgcactgc
    27361 gtaagttgta aatgccgttc cttgtagggc gtcatagcgt tcgacggcat taagtaaccc
    27421 aatgccgccc gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag
    27481 gcgcaatgct tcgtgacgca ccagcgggac ataacgctgc catagcgagt gtttatccat
    27541 tacaccttca gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc
    27601 ggcatgatta tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg
    27661 ttatttgggg gttaccggta aattgcgggc agaaaaaacc ccgccggtgg cgggggttga
    27721 gcgataagtg taaattaacg cagcagagac agtacgttct gcgtggtctg gttagcctgc
    27781 gccagaacag aggtacccgc ttgttgcagg atctgcgcac gagacatgtt agacacttcg
    27841 gtcgcgtagt cagcatcttc gatacggcta cgggcagaag acaggttgtt tacggtgttg
    27901 ccaaggttgg tgatagcaga gtcgaaacgg ttttgtactg caccgaggtc agaacgcaga
    27961 ttgtcaactt tagccaatgc tttgtcgata gtttcgagcg ggttggtggt agattgcaac
    28021 gattttgctg catcttcgtt taccagaatc gggctaccac cttctgattt gctcagatac
    28081 atgactttat tgttaccaga agcagtctcc gttatcgttt taccatctgc actaacatcg
    28141 taagttgcac cgttaacaac taacgtgctt cctgttttct tagctgcatt gagatcaaga
    28201 tcggaaagtg tggcagcctt gttatcaact ttggttgtcg tataaaggcc agcgctattt
    28261 ttgtaaactg tctcactcgt aatcaaagaa gcttttttgt cagcatctgt cacagtaaat
    28321 gtcactgact taccatcaat ttctgcagaa tatacaccat tcccattgcc atccgctgca
    28381 gttgttgttg tgaattcaac tccattaaac gtgaatttat cattctgtgc tttattctta
    28441 gccgctgctg cgagtgctgt agcatcaatc cctgtaccag ctatgtttgt gtcagattta
    28501 gttgtcagtg aaccatctgc tgcactaaca aaaatatcat tccctgtagt atctttaaca
    28561 gctccgctat ctacgttaac agtataagca tcaccgccaa catcatagtt atcagtacct
    28621 gtcgctttaa atttagaaat caggtcactg ccagttgctt tctgcgcgcc atcgatatta
    28681 aaaccgtcca ggccgagagt tttcgcatca atttttgcca gattgatagt gatggtttca
    28741 ccatcattag caccaacctg aattttcatt tcattatttt cagcaaggac tttcacgccg
    28801 ttaaactgag tttgctcaga tacacggtca atttcttcca gacgttgagt aatttcagcc
    28861 tggatagaag aaagatcgct gtcagagtta gtaccgttag ttgcctgaac agtcagttca
    28921 cgtacacgct gcaggttgtt gttaatttca ttcagcgcac cttcagtggt ctgcgcaaca
    28981 gaaataccat cattcgcgtt acgggaagcc tgagtcagac ctttaatatt tgctgtaaaa
    29041 cggttagcaa tcgcctgacc tgctgcgtca tctttagcac tgttaatacg caggccagaa
    29101 gagagacgtt caatggcgga gctcagagaa gactgagatt tgttcaggtt attctgggtc
    29161 aacagcgaca ggctgtttgt attaatgact tgtgccatga tcttttcctt atcaattaca
    29221 acttgatgtt attgggctgt tgcccacggt ttctcaccgt accccttgta tcggcacctg
    29281 aatttcgaac tttagaaaat tttagtttct ctcctgtttt ttcttaacgg ctgaaatatc
    29341 cgtaatatac aactctactc tgcgcatcat cttcgtgaat caactattaa actttgcgca
    29401 caaattgccg ataacccgct tatctactgt ttgcaatcaa aaggaattag gtatggcttc
    29461 atttacatct cttggcgttg gttcgaatct gccgctggat acattgctaa acaacctgac
    29521 catcgctgaa aaaaaacgct taaacccaat tactcagcaa cagagtgaca ataccgcacg
    29581 tctgacagcc tatggcacat taaaaagtgc attagaaaaa ttccagactg caaatactgc
    29641 gttaaataaa gccgatctgt tcaaaagcac taccgttacc agtagcaatg aagaccttaa
    29701 agtttccacc gaagcaggtg ctgcccccgg tatctatacc atcagcgtaa cccaactggc
    29761 gcaggcgcaa tctttacgaa ctgattcccc gacaatcatt gcatcaacaa aagatgcact
    29821 gggtgatgag tcatcagata cacgcacaat aaaaattacc caagacggtc gtaaagagcc
    29881 gctggaaatc aagctcaata aagatcagac gtcattggat gagatcagca aagccattaa
    29941 cgatgccgat agtggaattt ccgctagtat cgttaaagtc aaagatggta actatcaatt
    30001 ggtattgacc gccagtgaag gtctggccaa taaaatgacc atatctgttg agggtgacag
    30061 taaattaaat gatctgctgg cttatgacag caagaccaac accgggaata tgaaggaact
    30121 ggttaacgcg caaaatgcac agctaaacgt taacggtatc gatattgaac gttcaagtaa
    30181 caaaattacc gatgctcctc agggagtgac tctcgatttg accaaaaaag tcactgacgt
    30241 ccgggtcacg gtgacaaaaa gcaacgataa agcaaccgaa gcaattaaag gctgggtgga
    30301 ttcatataac tccctcattg acacctttaa caccctgaca aaatataaag aagtagaccc
    30361 aggtgctgaa gcacaagata aaaataatgg tgcacttctc ggagacagcg ttgtacgtac
    30421 aattcaaagc ggcatccgcg cacaatttgc aaacggtgcc agcgacagtg ccttcaaaac
    30481 attaaacgag atcgggatta agcaggacgg cacgacgggt aagctgaaaa ttgatgatga
    30541 taaactcaaa aaagtcctga atgaaaatac agcatccgta cgtgaattgc tggtaggcga
    30601 tggtaaagaa acaggaatta ccaccaaaat tgccaccgag gtaaaaggtt accttgccga
    30661 tgatggcatt attgatagcg cacaggacag tattaacgcc acactgaaaa aactgaccaa
    30721 acagtatcta tccgtcagtg ccagcattga tgacaccgtg gcgcgttaca ctgcacagtt
    30781 tacgcaatta gataccatga tgagcaaact gaataacacc agtacctatc tcagccaaca
    30841 atttactgca atgagtaatt cctgatcacg gaggtcagca tgtataccgc gagcggctca
    30901 aaagcctatg cacaaattgg cgtcgaaagc gccgtaatga gcgccagcca gcagcagctg
    30961 gtcaccatgc tatttgatgg agtgctgagc gcactggtta gagcacgcct gtttatgcag
    31021 gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag cgatcaacat cattgagaac
    31081 ggactgcggg tgagtcttga tgaagagagc aaagacgaat taacccaaaa cttgattgct
    31141 ctttatagct atatggtcag gcgcttgctg caagccaatt tacgcaacga tgtctccgca
    31201 gtcgaagaag tggaagcatt aatgcgcaat attgccgatg cctggaaaga gtcgttactc
    31261 tccccttctt tgattcagga cccagtctga tgaaccacgc accgcatttg tatttcgcct
    31321 ggcaacaact cgtcgaaaaa agccagctca tgttacgcct ggcaacagaa gaacaatggg
    31381 acgaactcat cgccagcgag atggcgtatg tgaatgcggt gcaggagatt gcacatctga
    31441 ctgaagagat tgacccgtcc accacgatgc aggagcagct ccgcccgatg ctgcgcctga
    31501 ttctcgacaa cgaaagcaag gtaaagcagt tattacagat tcggatggat gaactggcga
    31561 aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag tgcctatggt gatcagggcg
    31621 gctttgtgct ggctccgcag gataacctct tttgaatctg aacgagtcga tggctcgcga
    31681 ataatccgat tacggctacg cttctaatgt tccccttgaa tggagtcgaa gaatgcgtaa
    31741 tcccacgctg ttacaatgtt ttcactggta ttacccggaa ggcggtaagc tctggcctga
    31801 actggccgag cgcgccgacg gttttaatga tattggtatc aatatggtct ggttgccgcc
    31861 cgcctataaa ggcgcatcgg gcgggtattc ggtcggctac gactcctatg atttatttga
    31921 tttaggcgag tttgatcaga aaggcagcat ccctactaaa tatggcgata aagcacaact
    31981 gctggccgcc attgacgccc tgaaacgtaa tgacattgcg gtgctgttgg acgtggtagt
    32041 caaccacaaa atgggcgcgg atgaaaaaga agctattcgc gtgcagcgtg taaatgctga
    32101 tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa ggctggacgc gttacacctt
    32161 ccccgcccgt gccgggcaat actcgcagtt tatctgggat ttcaaatgtt ttagcggtat
    32221 cgaccatatc gaaaaccctg atgaagatgg catatttaaa attgttaacg actacaccgg
    32281 cgaaggctgg aacgatcagg ttgatgatga attaggtaat ttcgattatc tgatgggcga
    32341 gaatatcgat tttcgcaatc atgccgtgac ggaagagatt aaatactggg cgcgctgggt
    32401 gatggaacaa acgcaatgcg acggttttcg tcttgatgcg gtcaaacata ttccggcgtg
    32461 gttttataaa gagtggatcg aacacgtaca ggaagttgcg ccaaagccgc tgtttattgt
    32521 ggcagagtac tggtcgcatg aagtcgataa actgcaaacg tatattgatc aggtggaagg
    32581 caaaaccatg ctgttcgatg cgccgctgca gatgaaattc catgaagcat cgcgcatggg
    32641 gcgcgactac gacatgacgc agattttcac gggtacatta gtggaagccg atcctttcca
    32701 cgccgtgacg ctcgttgcca atcacgacac ccaaccattg caagccctcg aagcaccggt
    32761 cgaaccgtgg tttaaaccgc tggcgtatgc tctaattttg ttgcgggaaa atggcgttcc
    32821 ttcggtgttc tatccggacc tctacggtgc gcattacgaa gatgtcggtg gtgacgggca
    32881 aacctatccg atagatatgc caataatcga acagcttgat gagttaattc tcgcccgtca
    32941 gcgtttcgcc cacggtgtac agacgttatt tttcgaccat ccgaactgca ttgcctttag
    33001 ccgcagtggc actgacgaat atcccggctg cgtggtggtc atgtcgaacg gggatgatgg
    33061 cgaaaaaacc attcatctgg gagagaatta cggcaataaa acctggcgtg attttctggg
    33121 gaatcggcaa gagagcgtag tgaccgacga aaacggcgaa gcgaccttct tttgcaacgg
    33181 cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa attcatcccc ggcggcaagc
    33241 cggggagatt tcattacggc agtggcgtcg gtaacggcgc tttatccagc gcggcggcac
    33301 actctgccgt cgggcgatcc acacgctcca tcgtcatacc atcgtactca atggtgttac
    33361 cgtcacgctc aacctcgtac agctcacgtt ttacggtaac gttagtgaga tcatcagaca
    33421 tcaatgtcaa cttgcccggc acggcaatca cccgctgcca ctgacggcaa tccagggtat
    33481 cgccctcttt agtgacaatc aggctgccta tcgcttccgg actgaccagc gcacgttgcg
    33541 gccctttggt ctgccagtag cccgctagcc agtccggcgc gggggttttc acgacattgt
    33601 tatagttttc gacttcggca cagcccgcca gcaacatgag cgcgcttgca attgcgagtt
    33661 ttttcatcat tcgtcctgca tgagaagaaa agaggattgt ggcattaaag ccctgatgtc
    33721 gccagccttt ccaggttcag agagaaattt gatctaacta ttatttttgt gaattattac
    33781 cccgaaaata aatttgcatg atgtatgatt cgcgattctt gtttcaaacc ttctgagttc
    33841 agaggctaca ttcatgtcat ggcagcaatt caaacacgcc tggttgatta aattctgggc
    33901 acccatccct gcggtcatcg cggcgggtat tctctccact tactattttg gcattactgg
    33961 caccttttgg gctgtcacgg gtgaatttac ccgttggggc ggccagctcc tgcagctgtt
    34021 tggcgtccat gctgaagagt ggggttattt taaaattatc catctggaag gatcgccatt
    34081 aacccgcatc gacgggatga tgatcctcgg catgtttggc ggctgctttg ccgcagcgct
    34141 gtgggccaac aatgtcaaac tgcgcatgcc acgcagccgt atccgcatta tgcaggccat
    34201 tattggcggt attatcgctg gttttggcgc gcgtctggca atgggctgta acctggcggc
    34261 gttctttacc ggtattcctc agttctcgct gcatgcctgg ttctttgcca tcgccactgc
    34321 cattggttca tggtttggcg cacgctttac cctgctgccc atcttccgta ttcccgtgaa
    34381 aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa ccggatcagg cgcggcgtcg
    34441 ttttcgtctc gggatgctgg tctttttcgg tatgctgggc tgggcgctgc tcacggcgat
    34501 gaaccaacca aaactggggc tggcaatgct gtttggcgtc ggctttggtt tactgattga
    34561 acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg tggatcaccg gacgtaccca
    34621 tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc atcgggatct tcagttacgt
    34681 acagttaggc gttgaaccca aaatcatgtg ggcgggacca aacgcggtaa ttggtggttt
    34741 actgtttggt tttggcatcg tgctggcagg cggctgcgaa accggctgga tgtaccgcgc
    34801 ggtagaaggc caggtgcact actggtgggt cggtctgggc aatgtgatcg gctcgacgat
    34861 tctggcgtat tactgggatg atttcgctcc ggcgctggcc accgactggg acaaaatcaa
    34921 cctgctgaaa acctttggcc cgatgggtgg cctgctggtg acatatttgc tgttgtttac
    34981 tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc cgccgtgcgg caccgcagac
    35041 tgctaaggag atcgcatgaa aaatatcgtt cctgattacc gccttgatat ggtgggtgaa
    35101 ccctgccctt atccggcagt cgcaactctt gaggcgatgc cgcagttaaa aaaaggggaa
    35161 atcctggaag tggtgagcga ctgtccacag tcgatcaata atattccact ggatgcgcgt
    35221 aatcacggct atacggtgct ggatattcag caagacgggc cgaccattcg ttatttaatt
    35281 cagaagtaat ccctcattcc cgctgtggcc cacagcggga acatcgttgc ctctgacaca
    35341 tagattttac ccgccccctc tcctattctt ttcagtatga tctcaggagg tttctatgtg
    35401 tggacgcttt gcccaatccc aaacgcgtga agattacctt gcgcttctcg cggaagatat
    35461 tgaacgcgat attccctacg atcccgaacc cattggcaga tacaacgtcg cgccgggaac
    35521 caaagttctt ctgctcagtg aacgtgatga acaccttcat ctggatccgg ttttctgggg
    35581 atatgctccc ggatggtggg ataaaccgcc gctgattaac gcccgcgtag aaactgcggc
    35641 caccagtcgt atgtttaaac cgctctggca acatggtcgg gcaatctgtt ttgccgatgg
    35701 ctggtttgag tggaaaaaag aaggcgacaa aaaacagcct tattttatct atcgcgctga
    35761 tggacaacct gtttttatgg ccgcgatagg tagcacaccg tttgagcgcg gtgacgaagc
    35821 cgaaggattt ttaattgtca ctgcagcagc agaccagggt ctggtagata ttcatgaccg
    35881 ccgcccgctg gttctgtcgc cagaagctgc acaggaatgg atgcggcaag agattggcgg
    35941 taaagaagca tcagaaattg ctaccaacgg ctgtgtgcca gcaaaccagt tcacctggca
    36001 cccggtatcg cgcgcggtgg gtaatatcaa aaaccaggga gcggagttaa ttcaaccggt
    36061 ttgataagcc aggagactat tgatcaataa tactgcgtca taagaaatct ctattagaca
    36121 atagtagacg ctccttccac atgcgccaca agaaacctct attccagtga cacaattacg
    36181 cctgattatt tccctagccc ggaatatgaa taaaaacatc aacaaagatt aaattattca
    36241 aacgttactg ttgcgtctgc ggtcgtagca acatccgacg tggtgatgag tgttaaaaga
    36301 gacactctga tgttcagagg ggttatcgcg tttacggatc gtggatggca gaaaactttc
    36361 aggatcaggt atccatttta aaccagaaat tgagtgagtt tgccccatcc atgccccaag
    36421 cggtgagatt ggaagtaatt aacaacaggt tacaaaacct acacctgcat ggacatcact
    36481 tcctgatacg ccgccaccag cttattacgc acctgaatcc ccatttgcat agaaactgag
    36541 gctttttgca tatcggtcat cacatcgttt aacgccacgc cgggttcacc gagggtgaat
    36601 ttttctgcct gcgtgcgggc agctgtttgt gtatcgctaa tgcgatcgag cgtggcgtgc
    36661 agctgcccgg caaaactaat ggtcggttgc ggcagtgaat cctgcgcacg cgcactcatc
    36721 gccgtagcct gtaactggct gataacccct tcaatcccct gtatcgctga cattttcgtc
    36781 tcctggataa tttctggtag caaagcctac cagtaagtca ataagataaa ggcgctaaat
    36841 aacaacaaaa aaacgggttt attggcggat agaaaaaaac gaaagcacaa ataatggcag
    36901 cgtcaatttt tcgagtttgc tgacccggga gtgagtcttg ttccactttg ccaataacgc
    36961 cgtccataat cagccacgag gtgcgcgatg aatgcgactg cagcccagac aaaatctctt
    37021 gagtggctta atcgcctgcg tgcgaatccg aaaattccat tgattgttgc cggttccgcg
    37081 gcagtggcgg tcatggtcgc actgatcctg tgggcgaaag cccccgacta ccgcacatta
    37141 ttcagcaatc tttccgatca ggatggtggc gcaattgtca gccaactgac gcaaatgaat
    37201 attccttacc gcttcagcga agccagcggc gctattgaag ttccggcaga taaagttcac
    37261 gaactgcgtc tgcgcctggc acaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa
    37321 ctactcgatc aggaaaagtt tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg
    37381 gcgctggaag gcgagctttc tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc
    37441 gtacatctgg caatgccgaa accgtcttta ttcgtccgtg aacaaaaatc cccttctgca
    37501 tcggtgacgg taaatctgtt acccggccgc gcactcgatg aagggcaaat tagcgccatt
    37561 gtgcatctgg tttccagcgc cgttgctggt ctgccgccgg gaaacgtcac gctggtggat
    37621 cagggcggac atctgttaac ccagtccaat accagcgggc gcgatcttaa tgatgctcag
    37681 ttgaaatatg ccagcgatgt cgaaggccgt attcagcggc gtattgaagc aatcctgtcg
    37741 cctattgttg gtaacggtaa tattcacgcc caggtcacgg cgcagctgga cttcgccagt
    37801 aaagaacaaa cggaagaaca gtatcgccct aacggtgatg aatctcatgc ggcgcttcgt
    37861 tcacgccagc ttaatgagag cgagcaaagc ggttccggtt atccgggcgg cgtaccgggg
    37921 gcgttgtcga atcaaccggc acctgcgaat aacgcgccaa tcagcacgcc tccggcaaat
    37981 caaaataacc gccagcagca ggcgagcacc accagcaata gcgggccgcg tagcacacag
    38041 cggaatgaaa ccagtaacta cgaagtcgat cgcaccattc gtcataccaa aatgaacgtg
    38101 ggcgatgtgc aacgtctgtc agtcgcggta gtggtgaatt acaaaacctt gccagacggt
    38161 aaaccattgc ctctcagcaa cgaacagatg aagcaaattg aagatctgac ccgcgaggcg
    38221 atgggctttt ctgaaaaacg cggtgactcg ctcaatgtcg ttaactcgcc gttcaatagc
    38281 agtgacgaaa gcggcggaga actgccgttc tggcaacagc aagcgtttat cgatcagttg
    38341 ctggctgccg gtcgctggtt gctggtactg ctggtggcgt ggctgctgtg gcggaaagcg
    38401 gtacgcccgc agttaacacg tcgcgccgag gcgatgaaag ctgtacagca acaggcgcag
    38461 gcccgtgagg aagtggaaga tgcggtggaa gtccgcctga gcaaagacga acaactccaa
    38521 caacgacgcg ctaaccaacg tctgggggca gaagtcatga gccagcgtat ccgtgaaatg
    38581 tctgataacg atccgcgcgt ggtggcgctg gtcattcgcc agtggataaa taacgatcat
    38641 gagtaacctg acaggcaccg ataaaagcgt catcctgctg atgaccattg gcgaagaccg
    38701 ggcggcagag gtgttcaagc acctctccca gcgcgaagtg caaaccctga gcgctgcaat
    38761 ggcgaacgtc acgcagatct ccaacaagca gctaaccgat gtgctggcgg agtttgagca
    38821 agaagctgaa cagtttgccg cactgaatat caacgccaac gattatctgc gttcggtatt
    38881 ggtcaaagct ctgggtgagg aacgtgccgc cagcctgctg gaagatattc tcgaaactcg
    38941 cgataccgcc agcggtattg aaacgctcaa ctttatggag ccgcagagtg ccgccgatct
    39001 gattcgcgat gagcatccgc aaattatcgc caccattctg gtgcatctga agcgcgccca
    39061 ggccgccgat attctggcgt tgttcgatga acgtctgcgc cacgacgtga tgttgcgtat
    39121 cgccaccttt ggcggcgtgc agccagccgc gctggcggaa ctgaccgaag tactgaatgg
    39181 cttgctcgac ggtcagaatc tcaagcgcag caaaatgggc ggcgtgagaa cggcagccga
    39241 aattatcaac ctgatgaaaa ctcagcagga agaagccgtt attaccgccg tgcgcgaatt
    39301 cgacggcgag ctggcgcaaa aaattatcga cgagatgttc ctgttcgaaa atctggtgga
    39361 tgtcgacgat cgcagcattc agcgtctgtt gcaggaagtg gattccgaat cgctgttgat
    39421 cgcactgaaa ggggccgagc agccactgcg cgagaaattc ctgcgcaata tgtcgcagcg
    39481 tgccgccgat attctgcgcg acgatctcgc caaccgtggt ccggtgcgtc tgtcgcaggt
    39541 ggaaaacgaa cagaaagcga ttctgctgat tgtgcgccgc cttgccgaaa ctggcgagat
    39601 ggtaattggc agcggcgagg atacctatgt ctgataatct gccgtggaaa acctggacgc
    39661 cggacgatct cgcgccacca caggcagagt ttgtgcccat ggtcgagccg gaagaaacca
    39721 tcattgaaga ggccgaaccc agccttgagc agcaactggc gcaactgcaa atgcaggccc
    39781 atgagcaagg ttatcaggcg ggtattgccg aaggtcgcca gcaaggtcat aagcagggct
    39841 atcaggaagg actggcccag gggctggagc aaggtctggc agaggcgaag tctcaacaag
    39901 cgccaattca tgcccggatg cagcaactgg tcagcgaatt tcaaactacc cttgatgcac
    39961 ttgatagtgt gattgcttcg cgcctgatgc agatggcgct ggaggcggca cgtcaggtca
    40021 tcggtcagac gccaacagtg gataactcgg cactgatcaa acagatccaa cagttgttgc
    40081 agcaagaacc gttattcagc ggcaaaccgc aactacgcgt gcaccccgat gatctgcaac
    40141 gtgtggatga tatgctcggc gcaaccttaa gtttgcatgg ctggcgcttg cggggcgatc
    40201 ccacactcca tcctggcggc tgtaaagtct ccgccgatga aggcgatctc gacgccagtg
    40261 tcgccactcg ctggcaagaa ctctgccgtc tggcagcacc aggagtggtg taatgaccac
    40321 gcgcctgact cgctggctaa ccacgctgga taactttgaa gccaaaatgg cgcagttgcc
    40381 tgcggtacgt cgctacgggc gattaactcg cgctaccggg ctggtgctgg aagccaccgg
    40441 attacaattg ccgctcggcg caacctgtgt cattgagcgc cagaacggca gcgaaacgca
    40501 cgaagtagaa agcgaagtcg ttggctttaa cggtcaacgg ctgtttttaa tgccgctgga
    40561 ggaagtcgaa ggtgtcctgc ccggcgcgcg tgtttatgcc aaaaacattt cggcagaagg
    40621 gctgcaaagc ggcaagcagt tgccgctcgg tccggcgtta ttaggtcgtg ttctggacgg
    40681 cagcggtaaa ccgctcgatg gcctcccctc ccccgatacg acggaaaccg gtgcgctgat
    40741 taccccacca tttaacccgt tgcaacgtac accgattgaa catgtgctgg acaccggcgt
    40801 gcgcccaatc aatgccctgc ttaccgttgg gcgtgggcag cgtatggggc tgtttgccgg
    40861 gtccggcgtt ggtaaaagtg tgctgctggg gatgatggca cgttacaccc gcgccgatgt
    40921 cattgtcgtg ggtttgattg gtgaacgcgg acgcgaagta aaagatttta ttgagaatat
    40981 cctcggtgcc gaagggcgtg cacgctccgt ggtgattgcc gctccggcgg atgtctctcc
    41041 gcttctgcga atgcaaggtg ccgcctatgc cacgcgaatt gccgaagatt ttcgcgatcg
    41101 tggtcagcat gtgttgctga ttatggactc cctcacccgc tacgcgatgg cccagcgtga
    41161 aattgcgctg gcgattggcg aaccacctgc cactaaaggt tatccaccgt cggtgtttgc
    41221 caaattaccg gcactggtcg agcgtgccgg aaatggaatt agcggcggcg gctcgattac
    41281 cgcgttttat accgtgctca ccgaaggcga tgaccagcag gaccccattg ccgactctgc
    41341 gcgggcaatc ctcgacggtc acattgtgct gtctcgccga ctggcggaag ccgggcacta
    41401 tccggctatc gatattgaag cgtcgatcag ccgcgcaatg acggcgttga tcagtgagca
    41461 acattacgcg cgagtgcgca ccttcaaaca gctgttgtcg agttttcagc gtaaccgcga
    41521 tctggttagc gtcggcgcgt atgccaaagg cagcgatccg atgctcgaca aagccatcgc
    41581 cctgtggccg cagctggagg gctatttgca acaaggcatt tttgaacgcg cggactggga
    41641 agcgtctctc caggggctgg agcgtatttt cccgacagtg tcataaccca ggagataacg
    41701 gcagatggca gaacatggcg cgctggcgac cctgaaagat ctggcagaaa aagaggtaga
    41761 ggatgccgcg cgcctgctgg gtgaaatgcg tcgcgggtgt cagcaggcgg aagaacagct
    41821 caaaatgctg atcgattatc agaatgaata tcgcaataac ctcaacagcg atatgagtgc
    41881 cgggatgacc agcaaccgct ggatcaacta tcagcagttt atccagacgc tggaaaaagc
    41941 cattactcag catcgccagc aacttaatca gtggacgcag aaagttgaca ttgccctgaa
    42001 cagctggcga gaaaagaaac aacgtttgca ggcctggcag acactgcagg aacggcaatc
    42061 cacggcggca ctgcttgcag aaaaccgcct cgatcagaaa aagatggatg agttcgccca
    42121 gcgcgccgcc atgaggaaac ctgaatgatt cgcttagcgc ccttaattac cgccgacgtt
    42181 gataacgcca cattgcctgg cggcaaagcc agcgatgctg cacaagattt tctcgcgttg
    42241 ttgagcgaag cattagcagg cgagacaact accgacaaag cggcccccca gttgctggtg
    42301 gcaacagata agcccacgac aaaaggcgag ccgctggtca gcgagattct tgccgatgca
    42361 caacaagcgg atttactgat ccctgtggat gaaacaccgc ctgtcatcaa cgacgaacaa
    42421 tccacatcaa caccattaac caccgctcaa acgatgacgt tggctgcggt ggctgacaac
    42481 aatacggcaa aagacgaaaa agcggatgat ctgaatgaag acgtcaccgc aagcctgagc
    42541 gccctttttg cgatgttgcc gggttttgac aatacgccca aagtgactga tgtgccgtca
    42601 accgtgttac cggcagagaa accaacgctc ttcacaaaac tgacttctgc gcaactcaca
    42661 acagcacagc ctgatgatgc ccccggcacg ccagctcagc cattaacacc gctggtagca
    42721 gaagcccaga gtaaagcgga agtcatcagc acaccttcac cggtgaccgc tgccgccagc
    42781 ccgctaatca ctccacacca gacacagcca ctgcccaccg tcgccgtgcc tgtgttgagt
    42841 gcaccgctgg gttctcacga atggcaacaa tcattaagcc agcatatttc gctgttcacc
    42901 cgccagaggc aacaaagtgc agagttgcgt ctgcacccgc aggatttagg tgaagtgcaa
    42961 atctccctca aagtggatga taaccaggca caaatccaga tggtttcacc gcatcagcat
    43021 gtacgcgccg cgctggaagc agcgcttccg gtactgcgta cgcagctggc cgaaagtggc
    43081 attcagttag ggcaaagcaa catcagtggc gaaagcttta gtggtcagca gcaggccgct
    43141 tcccaacaac agcaaagcca acgcacagta aaccatgaac ctctggcggg ggaagacgac
    43201 gatacgcttc tggttcccgt ctctatacaa gggcgcgtaa caggcaacag cggcgttgat
    43261 attttcgcct aacgtcagag gtagcaccgt aatccgcgtc ttttccccgc tttgttgcgc
    43321 tcaagacgca ggataattag ccgataagca gtagcgacac aggaagaccg caacacatga
    43381 ctgattacgc gataagcaag aaaagcaagc gatcgctttg gatcccgatt ctggtattca
    43441 ttaccctcgc ggcctgtgcc agcgcaggtt acagctactg gcattcgcat caggttgccg
    43501 ctgacgacaa agcgcagcaa cgcgtcgtac cctccccggt cttctacgcg ctggatacct
    43561 tcacggtcaa tctgggcgat gcggatcgcg tgctttatat cggcataacc ctgcgcctga
    43621 aagatgaagc tacccgctcg cggctgagtg agtatttgcc ggaagtccgt agccgtttgc
    43681 tgttactgtt ttcgcgtcag gatgctgccg tactggcgac agaagaaggc aagaaaaacc
    43741 tgattgccga gattaaaacc acgctttcca ccccgcttgt tgccgggcaa ccgaaacagg
    43801 atgtcaccga cgtgctgtat accgctttta ttctgcgata acgacatggg cgatagtatt
    43861 ctttctcaag ctgaaattga tgcgctgttg aatggtgaca gcgaagtcaa agacgaaccg
    43921 acagccagtg ttagcggcga aagtgacatt cgtccgtacg atccgaatac ccaacgacgg
    43981 gttgtgcgcg aacgtttgca ggcgctggaa atcattaatg agcgctttgc ccgccatttt
    44041 cgtatggggc tgttcaacct gctgcgtcgt agcccggata taaccgtcgg ggccatccgc
    44101 attcagccgt accatgaatt tgcccgcaac ctgccggtgc cgaccaacct gaaccttatc
    44161 catctgaaac cgctgcgcgg cacagggctg gtggtgtttt caccgagtct ggtgtttatc
    44221 gccgtggata acctgtttgg cggcgatgga cgcttcccga ccaaagtgga aggtcgcgag
    44281 tttacccata ccgaacagcg cgtcatcaac cgcatgttaa agctggcgct tgaaggctat
    44341 agcgacgcct ggaaggcgat taatccgctg gaagttgagt acgtgcgttc ggaaatgcag
    44401 gtgaaattta ccaatatcac cacctcgccg aacgacattg tggttaacac gccgttccat
    44461 gtggagattg gcaacctgac cggcgaattt aatatctgcc tgcctttcag catgatcgag
    44521 ccgctacggg aattgttggt taacccgccg ctggaaaact cgcgtaatga agatcagaac
    44581 tggcgcgata acctggtgcg ccaggtgcag cattcacagc tggagctggt cgccaacttt
    44641 gccgatatct cgctacgcct gtcgcagatt ttaaaactga aacccggtga cgtcctgccg
    44701 atagaaaaac ccgaccgcat catcgcccat gttgacggcg tcccggtgct gaccagtcag
    44761 tacggcactc tcaacggtca gtatgcgtta cggatagaac atttgattaa cccgatttta
    44821 aattctctga acgaggaaca gcccaaatga gtgacatgaa taatccggcc gatgacaaca
    44881 acggcgcaat ggacgatctg tgggctgaag ctttgagcga acaaaaatca accagcagca
    44941 aaagcgctgc cgacgcggtg ttccagcaat ttggcggtgg tgatgtcagc ggaacgttgc
    45001 aggatatcga cctgattatg gatattccgg tcaagctgac cgtcgagctg ggccgtacgc
    45061 ggatgaccat caaagagctg ttgcgtctga cgcaagggtc cgtcgtggcg ctggacggtc
    45121 tggcgggcga accactggat attctgatca acggttattt aattgcccag ggcgaagtgg
    45181 tggtcgttgc cgataaatat ggcgtgcgga tcaccgatat cattactccg tctgagcgaa
    45241 tgcgccgcct gagccgttag tgatgaataa ccacgctact gtgcaatctt ccgcgccggt
    45301 ttctgctgcg ccactgctgc aggtgagcgg cgcactcatc gccattattg ccctgatcct
    45361 cgctgctgcc tggctggtaa aacggttggg atttgtccct aaacgcactg gcgttaacgg
    45421 tctgaaaatt agcgccagtg cttcgctggg cgcgcgtgaa cgggttgtgg tggtcgatgt
    45481 ggaagatgca cggctggtgc tcggcgttac cgcagggcaa atcaatctgc tgcataaact
    45541 tcccccttca gcaccaacgg aagagatacc gcagcccgat tttcagtcgg ttatgaaaaa
    45601 cttgcttaag cgtagcggga gatcctgatg cgtcgtttgt tgtctgtcgc acctgtcctt
    45661 ctctggctgg ttacgcccct cgccttcgcg caactgccgg gtatcaccag ccagccgctg
    45721 cctggcggtg gacaaagctg gtcgctcccg gtgcaaacgc tggtgttcat cacctcgttg
    45781 acgtttattc cggcaatttt actgatgatg accagtttca cccgcatcat cattgttttt
    45841 ggtttattgc gtaacgcgtt gggaacaccc tccgcgccac ctaaccaggt attgctggga
    45901 ctggcgctgt ttttgacctt ttttattatg tcgccggtga tcgataaaat ttatgtcgat
    45961 gcgtaccagc cattcagcga agagaaaatc tcaatgcagg aggcgctgga aaaaggggcg
    46021 cagccgctgc gtgagtttat gctgcgtcag acccgcgagg cggatttagg gttgtttgcc
    46081 agactggcga atactggccc gttgcaggga cctgaagctg tgccgatgcg cattttgctc
    46141 ccggcctacg tgaccagcga gttgaaaacc gcatttcaga taggcttcac gattttcatt
    46201 ccttttttga ttatcgacct ggtgattgcc agcgtgttga tggcattggg gatgatgatg
    46261 gttcccccag ccacaattgc tctgcccttt aaactgatgc tgtttgtact ggtggatggc
    46321 tggcaattgc tggtcggttc gctggcgcag agcttttaca gctagagagg caaaatgaca
    46381 cctgaatcgg tcatgatgat ggggactgaa gcgatgaaag tcgcgctggc actggctgcc
    46441 ccactgttgc tggtggcgct ggtcacgggt cttatcatca gtattttgca ggccgccacc
    46501 cagattaacg aaatgacgct gtcgtttatt ccgaaaatca tcgccgtatt tatcgccatt
    46561 attattgccg gaccgtggat gctcaatctg ttgctggatt acgtccgcac cttgttcact
    46621 aacctgccgt atatcatcgg gtaggccgta caatgatgca ggtaacaagc gatcaatggc
    46681 tttcctggtt aagcctgtac ttctggccgt tactgcgcgt gctggcgctc atttctaccg
    46741 caccaattct gagcgaacgc agcgtgccga aacgggtaaa actgggtctg gcaatgatga
    46801 tcacgttcgc tattgcccca tcattacctg ccaacgatgt tcctgttttt tcgttctttg
    46861 ctctgtggct ggccgtgcag cagatcctga tcggcattgc gcttggtttt accatgcaat
    46921 ttgcctttgc cgctgtgcga accgctggtg aaattatcgg tctgcaaatg gggctgtcat
    46981 ttgcgacgtt tgtcgatccg gccagccatc tcaatatgcc cgttttagcg cgtatcatgg
    47041 atatgctggc gttactgctg ttcctgacat ttaacggtca tttatggttg atttcactgc
    47101 tggccgatac ctttcacacc ctgccaattg gtggcgaacc gttgaacagc aatgcgtttc
    47161 tggcactcac caaagcaggg agtttgattt tccttaacgg gctgatgctg gcattaccgc
    47221 tcattactct gctgctgacc ctgaatctgg cattaggctt acttaatcgt atggccccgc
    47281 aattatccat ttttgttatt ggatttccat taattctgac tgtcggtatc tctttaatgg
    47341 cggcattaat gccgttaatt gcgccttttt gcgaacattt attcagtgag atgtttaatt
    47401 tgctggctga tattattagt gaattgccat taatataatt ccgtaacgtt tatcatgtta
    47461 tcctaaggat tatccgaaaa ataataccta cgaacatctt ccaggatact cctgcggcga
    47521 aatatttgtt ttaagctcac tcacatatcg caacatttac tttactttaa gacaattcca
    47581 ggcaaattat acaacacttt acgggatagt aagtccgcct gaaaaatcgc gagagtggcg
    47641 cattaggtga cccatgttgt tccgtttagt catgatgaaa tattcaggta aggggaatta
    47701 tcgttacgca ttgagtgagg gtatgccatg tcaacgatta ttatggattt atgtagttac
    47761 acccgactag gtttaaccgg gtatctgttg agtagagggg ttaaaaaaag agaaatcaac
    47821 gacattgaaa ccgttgatga ccttgccata gcttgtgatt cacagcaccc ttcagtggtg
    47881 tttattaatg aggactgttt catccacgat gcttctaaca gtcagcgtat caagctcatc
    47941 attaatcaac atcccaatac gttatttatc gtttttatgg caattgccaa tgttcatttt
    48001 gatgaatatc tattggtcag aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa
    48061 tctctcgacg atatccttgg cgatattctg aaaaaagaga caacgataac ctcgttttta
    48121 aatatgccga cgttatcatt gagccgaacc gaatcgagta tgttgcgaat gtggatggca
    48181 ggtcagggaa ccattcaaat ctctgaccaa atgaatatca aagccaagac cgtttcatcg
    48241 cataaaggta atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc
    48301 gtccgactga cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg
    48361 actggtggtt tcccaccagt caggccgaat tagattactc tgctttctcc acaaagatac
    48421 cgtcctgatg ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgaaa
    48481 ccaggtacat tgtgccttca ctaaactcct caactgccag taccacgcca ggacgacgcg
    48541 gaccgccatc cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc
    48601 tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc caggcgcgcg cgagtgctga
    48661 ttttctcgac ggtctatact taagagatgc caggcggact taacgactgg ccgcaacaac
    48721 agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa gagtacagcg ataccgcaaa
    48781 acgtgaggtc agcgtcgatg tcgatgccct gctggcggcg atcaatgaaa ttagcgaaag
    48841 cgaagttcat cgcagccaga acgattctga acacgttagc gtcgatggac gtgaatatca
    48901 tacatggcgt gaattggcgg atgccttcga actggatatt catgacttca gcgtctctga
    48961 agtgaatcgt tgaatgcaca ataaaaaaat cccgaccctg agggggtcgg gatgaaactt
    49021 gcttaagcaa gaagcactta aaaaattcgt tacaccagga aatctgatgt gttcatcacc
    49081 ttatccgcaa tttttttcgc tgacaagaaa atattcgtga tatgaatgat taacgctact
    49141 tatgaataga aatgtgacgc gcgtcacccc ttggtggctc tggcgtcaac gggatcgctc
    49201 ccgaaaaaat catctttgat aatttgtgct gttttgagaa tcatcctggt aaggggagta
    49261 ctgcgcaacc atgctttcaa ttcaacaacc actactggtt tttagcgatc ttgatggcac
    49321 cctgctggac agtcatagtt atgactggca accagctgcc ccctggctca gccgtttacg
    49381 cgaagcgaat gttcccgtca ttctctgtag cagtaagaca tcagccgaaa tgctgtactt
    49441 gcaaaaaacg ttggggctac aaggtttacc gctgattgcc gaaaatggcg cagtgatcca
    49501 gcttgctgag caatggcagg atatagacgg ttttccacgc atcatctcag gtattagcca
    49561 tggcgaaatc agccaggttt taaatacgct acgcgagaaa gaacatttta aattcacaac
    49621 ttttgatgat gtcgacgatg caaccatcgc cgaatggacg ggattaagcc gtagccaggc
    49681 ggcgctgacg cagctacatg aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg
    49741 tatggcacaa tttaccgctc gtctgaacga actgggcttg cagtttatgc agggtgcgcg
    49801 cttctggcac gtcctggatg cctctgccgg aaaagatcag gctgccaact ggattatcgc
    49861 gacctatcaa caatcgtcag gcaaacgccc aaccacactt ggcctgggcg atgggccaaa
    49921 cgatgcgccc ttactggagg taatggatta cgcggtgatt gtgaaagggt taaaccgtga
    49981 aggggtgcat ctgcatgatg aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc
    50041 ggaaggctgg cgtgaagggc tggaccattt tttctccgcc cattaagcgt tatcgctcgc
    50101 gaatacccga ttgcgcccgg cctgtttagc gagataaagt cgacggtcgg ccagtgactg
    50161 gagttgttca aaatcataat caccggtttc ctcgctgctg cttaccccca gcgaggcact
    50221 gatgcgtatc gtcgtactct tggcgatcaa catctctttt tcatttagct tcaggcgaat
    50281 acgttctgca acctccgcag cctgcgtcag gctcgcgcct ggcagaatca cacaaaactc
    50341 ctcaccaccg acccgcccgg caacatcctg cgctcgcaag gaactgctaa ttaatccggc
    50401 agcatgagaa agaacacggt cgcccgcctg atgaccaaag cggtcattaa tcgctttaaa
    50461 atggtcaagg tcgacctgaa tgacagaaaa aggatgttgg tgcgtctgac acaatttagc
    50521 gagcggacgg gctttttcga acagtgcgcc acggttatat aaacgcgtta aggtgtcgtg
    50581 ccacgcctgc cagtgcaacg agctttgcaa aacatacatg ttgctgacca tccggcgaat
    50641 cacataccag gagatgagta acatggtggt aaagagcgcc cacagcaggg ttaatgcaat
    50701 gctgatactg ccgaaatcgc cgcgcacgcc ttcgcttagc gtatggacgc gcaccagcac
    50761 accgtcgaaa tggtccagac gttcccagct aacatagcga ctgttcatac gaatgccgcc
    50821 ccgcgtgtca tgttccattg cctgcgccag caaggccagt tcacgaggat caaaaatatt
    50881 ccctgttgga tggtcaggat tggaagaggt caataatctc agcttactgt catagagctg
    50941 atactcacca tcgaggtttt tatcgatggc gtttcttaaa aattgctgca tggtacgcac
    51001 gggaatactc atccccagca cgccatacca gtagttttca ctgtctaccg gaacactgac
    51061 ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc gaagtgaacc agcgtaccgc
    51121 gcggtgacga ttttctcgtt gcgaatggcc gacaaaccag ggttgggtga catagccgta
    51181 ataacgcgtt ggtacattgc gcgtaaacaa ggtcggctgc gtcgaaacgt aaaatccggc
    51241 acgcgagaca tacatcgcct gttcaaccat cgacgaggag ttgtgtgcca gtcgcagcaa
    51301 gtaaccaact tccagtgcag cggtaatttc attgtcgagg ctttcatttt cgcgagacag
    51361 gagattcccc tcgctgacta atgcatccga cacaccattg actggcaggg tgcgtcgtcg
    51421 gttgagttcg atttgccagg cgtgctcgtc gcgatgctgt tcgaactcgg ttacggcatt
    51481 acgcagagag gtgaaatcca gtggcgctac gagagcttcg cgcatgccat tacgcaaaaa
    51541 gatcagtttg tcgacgttat actgcaaatg cttatcgagc gcgttggcta cgttttccag
    51601 atgattacgc tggctggaga tataggcatc ttccagcacc accacttcac gccaggtgag
    51661 caaggtggaa aaaagcaata ccacgataaa gcagagatta acgacatgac caggccccag
    51721 gcggcgtgcg agttttttca accagctctg gttttccatt tttgtctcgt gctgcaccct
    51781 gacgactccc aacctgcttt tttatgattc tggcataacc tggcgatagc gtaccgcaaa
    51841 acagcgataa ttttataatg gtggcatgat aagacgcgac agacatcgat agttttataa
    51901 cgtaggcatg ataagacgcg atagcgtcgc atcaggcaat tcagtgcaga cactggcaca
    51961 cccgaccgga tgcgacgtga gcgccacatc cggtcgggtg ttaatgatac cctttcaaac
    52021 ttcttccgca gaacgactcc cctcccccgg aacaagctcg gtttcatgaa aagcttcccg
    52081 cttgaccccg taaccgtcat accaacgaca ctcaaccata ccgctggagt atccggtgac
    52141 aatcatccgc gggccgccct ctttaaccgt aacttcctca ctaaccataa agctcatact
    52201 cgcctccttt tttcgagtga aacttgttca ccttagttga agatggcgaa ttttgcataa
    52261 tagcctgtgc gagtattaat gtgcctgacc gcgtattttc gctacggctt tcactcccag
    52321 caccacgatg ccgccgatga taaatccaag aatcagattt aaaacagtcg gtaatatcat
    52381 cgccaccact gcactttgct gcccggcgaa atgttcaatg gcgtgatgca gcggcgcaat
    52441 tccatgtacc acaatcccac cgccgacgag gaacatcgcc agcgtgccga caatcgataa
    52501 cgccttcatc aaccagggcg caatgatcaa taaccctttg cctaatgcct gcatcagcgc
    52561 gctggatttt tccgccagcc aataccccag gtcatcaatc ttaacgataa cccctaccag
    52621 accgtagacg cccacggtca ctaccagcgc gatgcctgaa agcaccagca cctgattaag
    52681 caacggcgct tcggccacaa tccccagcgt gatggcgacg atttccgcag acaatataaa
    52741 atcggtacga atcgcccctt ttattttgtc tttttcaaac ttcagcggat cctgcgccgc
    52801 cagcttctcc agacgctgct gactctgcgc cggatcttct ttatgtttac gcgcctccag
    52861 catatgcagc actttctcta ccccttcaaa gcagagaaac gcgccgccaa tcatcaacag
    52921 tggcgtaatc gcccacggga taaacgcact gatgatcagc gccagcggca ccagaatcac
    52981 tttattgatc agcgatcctt tcgccacgcc ccagaccacg ggaagttccc ggttggcgcg
    53041 cacgcctgca acttgttgcg cattgagcga taagtcatcc cctaatacac cggcggtttt
    53101 cttcgccgcc agtttgccca tcacggagat atcgtccagc agtgtggcga tatcatcgag
    53161 caacgtcagt aaactactcc cggccaaaag cactccctct catttttatt gttgaagggt
    53221 gtaagtatga agcaaaaacg gcagctctga tacttgcttc acaggtcaac atttatttaa
    53281 caatcaagtg caattatatc tctcgccagc agaatgattt tcacgtttac tatgaaccac
    53341 ctttttattt cagccgtgag ggattatgcg tttccgccag ttgttaccgc tttttggcgc
    53401 gctgtttgcg ttgtatatca tttggggctc aacctatttt gtcattcgga ttggcgtgga
    53461 aagctggcct ccgttaatga tggcgggcgt tcgattcctg gcagccggta ttttattgct
    53521 ggcatttttg ctactgcgcg gacacaaact tcccccgcta cgtccgctgc tcaatgccgc
    53581 gctgattggc ctgttattgc tggctgtcgg taatggcatg gtgacggttg ccgaacatca
    53641 aaatgttcct tccggcatcg ccgccgttgt ggttgcaacc gtgcccctct ttaccctgtg
    53701 cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta tcgccattgg
    53761 gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc cgtggggcgc
    53821 gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg gctcgcgcat
    53881 taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggctg gcgtggtgtt
    53941 aatgattgcg tcgatgattg cgggtgaaaa actgacggcg ctcccttccc tttcaggctt
    54001 ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccattaacg cttatatgta
    54061 tttaatccgc aacgtcagtc cggctctcgc caccagctac gcctacgtta acccagtggt
    54121 cgcggtcttg ctgggtacgg ggctgggtgg cgaaacgctg tcgaagattg aatggctggc
    54181 gctcggcgta attgttttcg cggtggtact ggtcacgttg ggaaaatatc tcttcccggc
    54241 caaacccgtt gtcgcgccag ttattccgga tgcatcaagc gagtaaatga atcccctgcg
    54301 tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc tcggtaagcg
    54361 ccgcatccgt cagcttctcg cgcccgcgta acgcacattc ccagacaatc aatacgcgcc
    54421 aaccgagttc ctgcaaatgg ctgatatcac gacgatcgcg ctcgacattt ttacctatct
    54481 tctccagcca aaaatctgtt cgcgtggcgg ggactttaaa caggtagcaa tggtgatggt
    54541 gccagaagca accgtgggca aatatcacac agcgaaattc atcaaggaca aaatccgggc
    54601 gtccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccagtt aacaaactgg
    54661 cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt ttgctgcggg
    54721 tggccttatc gtgaacgtcg accatgttgt gcctcttgct gacgcaacgc caccgcctgt
    54781 ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac caccgagtta
    54841 ccgaactggc gataagcctg agtgtccgaa accggaatgc ggaatttcgc ttctcccggc
    54901 gcttcaaaac ccattaagcg cgcacattcc cgaggcgtta accgacgtgg gcgatgttgc
    54961 tgattcagcg gatcgtcaaa gtctttctca cccgttgcca tatcccagcc gcgatcgatt
    55021 aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac gctttgcgga
    55081 ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg ttttttcgca
    55141 tatcgataga ggtacttcca cagtaccggc gtcaggatat atttcgcctc gaccatcggg
    55201 tccagcagct gcgccagcgt cactcgctgt gcagggaaac atttgctgat atcacgcaga
    55261 gtaaaatcgg ctttcagatt cagatcgcga cgaaaaccca ccagcacgat gcgttcacgg
    55321 tgctgcggca gaaaatgttt gccgtcgatg attttcggat cgtccggccc gttatcttct
    55381 gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg gaacgtttta
    55441 ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc cggacgacgc
    55501 gcgtcgataa tgcgcaccac atcaaagaac agcgtgccct gggtatcgca ggcaaaaccg
    55561 tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg ctgacaaggg
    55621 aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc cgccgccgcc
    55681 tcatcactca cgccttcttg atggctgagg gtgatgtcgc ggatatcctc attaaaatga
    55741 tgcgtcgccg ggtcgcaata atggttggct ttataagtgc gtaccgcatg tttgttccat
    55801 tcgctggtaa acacgcactg tccaccaatc gattcaaaac cgcgacggat gccgccaatt
    55861 ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccgg tggtttgggt
    55921 aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc cgagtcattc
    55981 gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc attaagctgc
    56041 gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc ctgtgcggca
    56101 ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt aaccggccat
    56161 ctgagatggc cgacaggtta acataattac tgccgtttag ggccacgcgc tcagccagca
    56221 ggtgaaaacg catctatcac cttgtgatcg ataccttcat tctcccccgc cagttcggca
    56281 ccgagcttcg ccataaactc gactaaaaat tgcgcgttgt gctgtgccag ctgcttgcct
    56341 cttgcggttt gcatggtttg cggtaatttc agtaactttg tctggaaatg atccagcgcg
    56401 tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcgccatc aaacagcgcc
    56461 aagcccaatg cccctgaaac ggcaaatacg cgcgccaggc caatcgcccc cagggcttcc
    56521 agccgatccg catcctggac gattttagct tccgttgtta agggggcgat ttgcgcgctg
    56581 aaactgtgag cggcaatggc atgacaaacg gcctcaattt tctccgccgg aaattgcacg
    56641 aactctt
//
LOCUS       DAZFJF010000017        48299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFJF010000017 DAZFJF010000000
VERSION     DAZFJF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.017"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            165..1382
                     /gene="alaA"
                     /locus_tag="ACHKAU_001198"
     CDS             165..1382
                     /gene="alaA"
                     /locus_tag="ACHKAU_001198"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO5530305.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1466..2065
                     /gene="yfbR"
                     /locus_tag="ACHKAU_001199"
     CDS             1466..2065
                     /gene="yfbR"
                     /locus_tag="ACHKAU_001199"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5530306.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2124..3956)
                     /locus_tag="ACHKAU_001200"
     CDS             complement(2124..3956)
                     /locus_tag="ACHKAU_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO5530307.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4043..4693)
                     /gene="hxpA"
                     /locus_tag="ACHKAU_001201"
     CDS             complement(4043..4693)
                     /gene="hxpA"
                     /locus_tag="ACHKAU_001201"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO5530308.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4704..5198)
                     /locus_tag="ACHKAU_001202"
     CDS             complement(4704..5198)
                     /locus_tag="ACHKAU_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO5530309.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5281..5736)
                     /gene="yfbV"
                     /locus_tag="ACHKAU_001203"
     CDS             complement(5281..5736)
                     /gene="yfbV"
                     /locus_tag="ACHKAU_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO5530310.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6074..7276
                     /gene="ackA"
                     /locus_tag="ACHKAU_001204"
     CDS             6074..7276
                     /gene="ackA"
                     /locus_tag="ACHKAU_001204"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO5530311.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7351..9495
                     /gene="pta"
                     /locus_tag="ACHKAU_001205"
     CDS             7351..9495
                     /gene="pta"
                     /locus_tag="ACHKAU_001205"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5530312.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9685..11205
                     /gene="yfcC"
                     /locus_tag="ACHKAU_001206"
     CDS             9685..11205
                     /gene="yfcC"
                     /locus_tag="ACHKAU_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO5530313.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11238..11780)
                     /gene="yfcD"
                     /locus_tag="ACHKAU_001207"
     CDS             complement(11238..11780)
                     /gene="yfcD"
                     /locus_tag="ACHKAU_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO5530314.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11838..12389)
                     /gene="yfcE"
                     /locus_tag="ACHKAU_001208"
     CDS             complement(11838..12389)
                     /gene="yfcE"
                     /locus_tag="ACHKAU_001208"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO5530315.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12445..13089)
                     /gene="yfcF"
                     /locus_tag="ACHKAU_001209"
     CDS             complement(12445..13089)
                     /gene="yfcF"
                     /locus_tag="ACHKAU_001209"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO5530316.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13225..13872
                     /gene="yfcG"
                     /locus_tag="ACHKAU_001210"
     CDS             13225..13872
                     /gene="yfcG"
                     /locus_tag="ACHKAU_001210"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO5530317.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13929..14291
                     /gene="folX"
                     /locus_tag="ACHKAU_001211"
     CDS             13929..14291
                     /gene="folX"
                     /locus_tag="ACHKAU_001211"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO5530318.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14312..15205
                     /locus_tag="ACHKAU_001212"
     CDS             14312..15205
                     /locus_tag="ACHKAU_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO5530319.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15253..16143)
                     /gene="rpnB"
                     /locus_tag="ACHKAU_001213"
     CDS             complement(15253..16143)
                     /gene="rpnB"
                     /locus_tag="ACHKAU_001213"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO5530320.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16340..17113)
                     /gene="hisP"
                     /locus_tag="ACHKAU_001214"
     CDS             complement(16340..17113)
                     /gene="hisP"
                     /locus_tag="ACHKAU_001214"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO5530321.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17121..17837)
                     /locus_tag="ACHKAU_001215"
     CDS             complement(17121..17837)
                     /locus_tag="ACHKAU_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5530322.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17834..18520)
                     /gene="hisQ"
                     /locus_tag="ACHKAU_001216"
     CDS             complement(17834..18520)
                     /gene="hisQ"
                     /locus_tag="ACHKAU_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO5530323.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18610..19392)
                     /gene="hisJ"
                     /locus_tag="ACHKAU_001217"
     CDS             complement(18610..19392)
                     /gene="hisJ"
                     /locus_tag="ACHKAU_001217"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO5530324.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19613..20395)
                     /gene="argT"
                     /locus_tag="ACHKAU_001218"
     CDS             complement(19613..20395)
                     /gene="argT"
                     /locus_tag="ACHKAU_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO5530325.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20661..21230)
                     /gene="ubiX"
                     /locus_tag="ACHKAU_001219"
     CDS             complement(20661..21230)
                     /gene="ubiX"
                     /locus_tag="ACHKAU_001219"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO5530326.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21325..22842)
                     /gene="purF"
                     /locus_tag="ACHKAU_001220"
     CDS             complement(21325..22842)
                     /gene="purF"
                     /locus_tag="ACHKAU_001220"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO5530327.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22879..23367)
                     /gene="cvpA"
                     /locus_tag="ACHKAU_001221"
     CDS             complement(22879..23367)
                     /gene="cvpA"
                     /locus_tag="ACHKAU_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO5530328.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23626..24288)
                     /gene="dedD"
                     /locus_tag="ACHKAU_001222"
     CDS             complement(23626..24288)
                     /gene="dedD"
                     /locus_tag="ACHKAU_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO5530329.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24278..25546)
                     /gene="folC"
                     /locus_tag="ACHKAU_001223"
     CDS             complement(24278..25546)
                     /gene="folC"
                     /locus_tag="ACHKAU_001223"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO5530330.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25616..26530)
                     /gene="accD"
                     /locus_tag="ACHKAU_001224"
     CDS             complement(25616..26530)
                     /gene="accD"
                     /locus_tag="ACHKAU_001224"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO5530331.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26686..27345)
                     /locus_tag="ACHKAU_001225"
     CDS             complement(26686..27345)
                     /locus_tag="ACHKAU_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5530332.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27428..28240)
                     /gene="truA"
                     /locus_tag="ACHKAU_001226"
     CDS             complement(27428..28240)
                     /gene="truA"
                     /locus_tag="ACHKAU_001226"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO5530333.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28240..29253)
                     /locus_tag="ACHKAU_001227"
     CDS             complement(28240..29253)
                     /locus_tag="ACHKAU_001227"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5530334.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29319..30455)
                     /gene="pdxB"
                     /locus_tag="ACHKAU_001228"
     CDS             complement(29319..30455)
                     /gene="pdxB"
                     /locus_tag="ACHKAU_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO5530335.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30554..31549
                     /gene="flk"
                     /locus_tag="ACHKAU_001229"
     CDS             30554..31549
                     /gene="flk"
                     /locus_tag="ACHKAU_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO5530336.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31546..32724)
                     /locus_tag="ACHKAU_001230"
     CDS             complement(31546..32724)
                     /locus_tag="ACHKAU_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5530337.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33008..34228)
                     /gene="fabB"
                     /locus_tag="ACHKAU_001231"
     CDS             complement(33008..34228)
                     /gene="fabB"
                     /locus_tag="ACHKAU_001231"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO5530338.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34387..36393
                     /gene="mnmC"
                     /locus_tag="ACHKAU_001232"
     CDS             34387..36393
                     /gene="mnmC"
                     /locus_tag="ACHKAU_001232"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO5530339.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36514..36792)
                     /locus_tag="ACHKAU_001233"
     CDS             complement(36514..36792)
                     /locus_tag="ACHKAU_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO5530340.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36826..37374)
                     /gene="epmC"
                     /locus_tag="ACHKAU_001234"
     CDS             complement(36826..37374)
                     /gene="epmC"
                     /locus_tag="ACHKAU_001234"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO5530341.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37374..38183)
                     /locus_tag="ACHKAU_001235"
     CDS             complement(37374..38183)
                     /locus_tag="ACHKAU_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO5530342.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38183..39007)
                     /gene="mepA"
                     /locus_tag="ACHKAU_001236"
     CDS             complement(38183..39007)
                     /gene="mepA"
                     /locus_tag="ACHKAU_001236"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO5530343.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39011..40096)
                     /gene="aroC"
                     /locus_tag="ACHKAU_001237"
     CDS             complement(39011..40096)
                     /gene="aroC"
                     /locus_tag="ACHKAU_001237"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO5530344.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40131..41063)
                     /gene="prmB"
                     /locus_tag="ACHKAU_001238"
     CDS             complement(40131..41063)
                     /gene="prmB"
                     /locus_tag="ACHKAU_001238"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5530345.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41229..41780
                     /gene="smrB"
                     /locus_tag="ACHKAU_001239"
     CDS             41229..41780
                     /gene="smrB"
                     /locus_tag="ACHKAU_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO5530346.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41853..42704)
                     /locus_tag="ACHKAU_001240"
     CDS             complement(41853..42704)
                     /locus_tag="ACHKAU_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO5530347.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(42706..43245)
                     /locus_tag="ACHKAU_001241"
     CDS             complement(42706..43245)
                     /locus_tag="ACHKAU_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5530348.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43242..43730)
                     /locus_tag="ACHKAU_001242"
     CDS             complement(43242..43730)
                     /locus_tag="ACHKAU_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5530349.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(43727..44236)
                     /locus_tag="ACHKAU_001243"
     CDS             complement(43727..44236)
                     /locus_tag="ACHKAU_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5530350.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44252..45004)
                     /locus_tag="ACHKAU_001244"
     CDS             complement(44252..45004)
                     /locus_tag="ACHKAU_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5530351.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45024..47669)
                     /gene="yfcU"
                     /locus_tag="ACHKAU_001245"
     CDS             complement(45024..47669)
                     /gene="yfcU"
                     /locus_tag="ACHKAU_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO5530352.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47751..>48299)
                     /locus_tag="ACHKAU_001246"
     CDS             complement(47751..>48299)
                     /locus_tag="ACHKAU_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5530353.1"
                     /translation="KTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVPDD
                     HKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYY
                     TIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLV
                     GAADAPDLGNFEANTTFQITYL"
BASE COUNT        11760 a        13108 c        12108 g        11323 t
ORIGIN      
        1 ctcattattt tgtcattatt aagttattaa cagcacaatc gagccttccc ctctggcaaa
       61 atcttattct gcagaccttc aaaacaccgt cctgggggag tacattgttc taagctgact
      121 tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc cccattgaaa
      181 aatccagcaa attagagaat gtctgttatg acatccgtgg tccggtgctg aaagaagcaa
      241 aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac ccagccccgt
      301 tcggttttga cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg cctaccgctc
      361 aagggtattg cgattccaaa ggtctttact ccgcgcgtaa agccatcatg cagcactacc
      421 aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggcaat ggtgtatcgg
      481 agcttatcgt tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg ttggttcctg
      541 caccagatta cccactatgg accgcggcgg tttcgctttc cagcggtaaa gcggtgcatt
      601 atctttgcga tgaatcctct gactggttcc cggacctcga tgatattcgc gctaaaatta
      661 cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc gcggtatatt
      721 ccaaagagct tttaatggag attgtggaga ttgcacgtca gcataatctc attatcttcg
      781 ccgatgaaat ttatgacaaa attctttacg acgacgctga gcatcactca attgcgccgc
      841 tggcacctga cctgctgacc attaccttta acggactgtc gaaaacgtac cgcgttgcag
      901 gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg
      961 aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca
     1021 ttcagaccgc gctgggtggt tatcagagca tcagtgaatt tattacccct ggcggtcgtc
     1081 tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga tattccgggc gtttcctgcg
     1141 tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga cgccaaacgc tttaacattc
     1201 acgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg ttggtgcaag
     1261 ggacggcatt caactggccg tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg
     1321 atgatatcga gctgtctttg agcaagttcg cgcgtttcct ttctggttat catcagctgt
     1381 aatcttaatt tcactgccgg agattgcatc cggcagcgtt atcccgccac aatgacctga
     1441 tgatgtcatc atacgtaagg tcactatgaa acagagccat ttttttgccc atctctcccg
     1501 cctgaaactc attaaccgct ggccgctcat gcgcaacgtg cggacggaaa atgtgtccga
     1561 acacagtttg caggtagcga tggtcgccca tgcgctggca gctatcaaaa atcggaaatt
     1621 tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg atgcctcaga
     1681 agtgctcacc ggcgatctcc ctactccggt gaaatacttc aattcgcaaa tcgctcagga
     1741 gtacaaggct attgaaaaaa tcgctcagca aaaactggtc gatatggttc cggaagagct
     1801 gcgggatatc tttgcgccgt taattgacga gcatgcatat agcgatgaag aaaaatcgct
     1861 ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac tcgcggccgg
     1921 aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgtcgcag
     1981 ccaggagatg gactacttca tggaagtatt tgttcccagc ttccatcttt cgctcgatga
     2041 gattagccag gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg
     2101 ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa
     2161 accgccatca cgataatggt gaacggtacc cccaacttca caaagtcact gaagctgtaa
     2221 tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa ccggtgtcat aaaggcggcg
     2281 gatgctgcca tcgccacgac catcgcgaat ggataaggcg acacccccat cgttttggca
     2341 gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagagat aaatagccca
     2401 ataaccgccg acaagacaaa caaacacccc agcatcatat gtggcccgta accaccgcca
     2461 atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa tgccacagca
     2521 aacggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt ataggcgctt
     2581 tcagcatcta tacagcggaa tttccccatc agcaggcagg cgatgatagc ggcaacagga
     2641 ttaggaattt catctgtcag cattaacgcc accattagca ccagacagaa aatggcatgg
     2701 ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgctacg
     2761 aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac aaccaggatg
     2821 atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac gccattgcgc
     2881 tttagcccca ccacattcag tccgtagcgg gtacgaaaac caatttcgcg caccgattta
     2941 ccaatcagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg
     3001 tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc
     3061 cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca
     3121 gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg
     3181 acgttagcgc cataacgctc acgtaatttg agatcatcca gccgttgacc tatcattggc
     3241 gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc acggataaga
     3301 tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc ccctttcagc
     3361 atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag gccaataggt
     3421 gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact gttgactacc
     3481 aggttcggcg gcgtcgccac cagcgtcatc atgccgctaa tcagcccggc aaaacttaac
     3541 ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg caacgcttaa caccacggga
     3601 ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc gacggtcagc
     3661 atcaacaaaa ccaacatttt gatttcactg ttgcccgcca ctttgaccag ccatgttccc
     3721 attacggtgg caacaccggt acggaccaaa ccatcgccaa taataaacaa ggcggcaatc
     3781 aggacaacgt taggatcaga aaagccggaa aatacttcgg ggaccgtcag cgttccgctt
     3841 aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct gcccgtcgca
     3901 aacaagacga tggcaacggc cagtaatgaa agaacccaaa tcaattcacc gttcacaact
     3961 tatccttgtt aattgagggg gatgacttga ttctgccata aaaaagcccc gacgagacgg
     4021 ggctaaatca tgatcaggtg tttcactgaa taataacatc gccatttggc tgtttggtca
     4081 cagtaatttg ctccagactg tggaggacca aatcgacctc attcaggcgc ggggtatctg
     4141 ccggagcgtt aaccgcaatg acatggcaac ccgccgccag gccagaaagc acgccagcgg
     4201 gagcatcttc caccaccaca cactcctgcg gcgcaagccc cagcagctgc gcacctaaca
     4261 gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggttaca aacacctctg
     4321 gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac cggcatggag ccagaagtca
     4381 caatggccca cggaatacct gctttattca aatgattgag taaggcgatt gcccccggaa
     4441 gcgcggtaat accttcggtt tccgtggcct cgatgtgctc cagacgcgta aactcggcgg
     4501 caatatcagc ctcggatttg cccgccataa aatggcgcaa agaggtgatc gcctgtttac
     4561 cgtgaatgaa agccagcacc tcttccggcg ctaacccatg acgtctggcc cagttgctcc
     4621 acgcccgttc taccgcaggc agggaatcca ccagcgttcc atcaagatca aacagaaaac
     4681 ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt gattaatttc gttggcgctc
     4741 aaatggtact gacgcgggca ggcatgccac acattaagca tgcgctgata tttttcccac
     4801 attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc
     4861 acattaacca taaagcggac ataaccgagg taacgtgctt cagtggcggc gtcaaagccg
     4921 aggaaggtga cacgacgttc atcgatggat tgctggtcct gcaaattaga ccaggaaaca
     4981 tgcaacgcat gatacatctc cataatgtcg atgatagtgc ggcaggtttc ttctttcagc
     5041 tcgccaaact cgcgatccaa ttcacgcatc tgtaatccgt aaccacgctc aataattgtt
     5101 tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca tagtcatcat cttgtactga
     5161 ttagacaaaa taagacgttg cgcgttggtc atttccattg ttgactcctg tatcactata
     5221 ctacggtgaa aaaaaagaag gctgagtatg ccttctttta tatgcgtaat caggggtcaa
     5281 ttacaaatca tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc
     5341 taatgcctgg taatcaggct tgccttcaac gggtgccaac acctgtccag actcctgcaa
     5401 tttaccgcga acttcataaa accagttaag gattgcaggg ggtaatggcg ttacagaacg
     5461 cttgcccagc caccacaatc cctgcatggg taaacttaag gcgaacagcg cagtggcaac
     5521 tgccggccca agctgaccgc ccagggcaat ctgccagcag agagtaaata cggcgatcgg
     5581 cggcataaaa cggatcgcat aacgcgtcat cttgataacg cgattttcga caaagaccgg
     5641 ggcaaggcgt ttttccagcg gccacgtctt tgagtaatgc tgtccccggc gaaacaagct
     5701 aaaaaaatta acagaacgat tatccggcgt tgacatgctt cacctcaact tcacatataa
     5761 agattcaaaa atttgtgcaa attcacaact cagcgggaca acgttcaaaa cattttgtct
     5821 tccataccca ctatcaggta tcctttagca gcctgaaggc ctaagtagta catattcatt
     5881 gagtcgtcaa attcatatac attatgccat tggctgaaaa ttacgcaaaa tggcatagac
     5941 tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg catgatgtta atcataaatg
     6001 tcggtgtcat catgcgctac gctctatggc tccctgacgt ttttttagcc acgtatcaat
     6061 tataggtact tccatgtcga gtaagttagt actggttctg aactgcggta gttcttcact
     6121 gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac ctttctggtt tagccgaatg
     6181 tttccacctg cccgaagcac gtatcaaatg gaaaatggac ggcaataaac aggaagcggc
     6241 tttaggtgca ggcgccgctc acagcgaagc gctcaacttt atcgttaata ctattctggc
     6301 acaaaaacca gaactgtctg cgcagctgac tgctatcggt caccgtatcg tacacggcgg
     6361 cgaaaagtat accagctccg tagtgatcga tgagtctgtt attcagggta tcaaagatgc
     6421 agcttctttt gcaccgctgc acaacccggc tcacctgatc ggtatcgaag aagctctgaa
     6481 atctttccca cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt tccaccagac
     6541 tatgccggaa gagtcttacc tctacgccct gccgtacaac ctgtacaaag agcacggcat
     6601 ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta acccaggaag cggcaaaaat
     6661 gctgaacaaa ccggtagaag aactgaacat catcacctgc cacctgggca acggtggttc
     6721 cgtttctgct atccgcaacg gtaaatgcgt tgacacctct atgggcctga ccccgctgga
     6781 aggtctggtc atgggtaccc gttctggtga tatcgatccg gcgatcatct tccacctgca
     6841 cgacaccctg ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag agtctggcct
     6901 gctgggtctg accgaagtga ccagcgactg ccgctatgtt gaagacaact acgcgacgaa
     6961 agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat acatcggtgc
     7021 ctacactgcg ctgatggatg gtcgtctgga cgctgttgta ttcactggtg gtatcggtga
     7081 aaatgccgcg atggttcgtg aactgtctct gggcaaactg ggcgtgctgg gctttgaagt
     7141 tgatcatgaa cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca acaaagaagg
     7201 tacccgtcct gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag
     7261 ccgcctgact gcctgatttc acaccgccag ctcagctggc ggtgctgttt tgtaacccgc
     7321 caaatcggcg gtaacgaaag aggataaacc gtgtcccgta ttattatgct gatccctacc
     7381 ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa
     7441 ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc gtaccggtgg cgatgcgccc
     7501 gatcagacta cgactatcgt gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg
     7561 aaaatgagct acgttgaagg tctgctttcc agcaatcaga aagatgtgct gatggaagag
     7621 atcgtcgcaa actaccacgc taacaccaaa gacgctgaag tcgttctggt tgaaggtctg
     7681 gtcccgacac gtaagcacca gtttgcccag tctctgaact acgaaatcgc taaaacgctg
     7741 aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca gctgaaagag
     7801 cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat caccggcgtt
     7861 atcgtcaaca aactgaacgc accggttgat gaacagggtc gtactcgccc agatctgtcc
     7921 gagattttcg acgactcttc caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa
     7981 gaatccagcc cgctgccggt tctcggcgct gtgccgtgga gctttgacct gatcgcgact
     8041 cgtgcgatcg atatggctcg ccacctgaat gcgaccatca tcaacgaagg cgacatcaat
     8101 actcgccgcg ttaaatccgt cactttctgc gcacgcagca ttccgcacat gctggagcac
     8161 ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct
     8221 tgcctggcag ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa
     8281 atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct gccggtattt
     8341 atggtgaaca ccaacacctg gcagacctct ctgagcctgc agagcttcaa cctggaagtt
     8401 ccggttgacg atcacgagcg tatcgagaaa gttcaggaat acgttgctaa ctacatcaac
     8461 gctgactgga tcgaatctct gactgccact tctgagcgca gccgtcgtct gtctccgcct
     8521 gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg
     8581 gaaggtgacg aaccgcgtac cgttaaagca gccgctatct gtgctgaacg tggtatcgca
     8641 acttgcgtac tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta
     8701 gaactgggtg cagggattga aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt
     8761 cgtctggtcg aactgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg
     8821 gaagacaacg tggtgctcgg tacgctgatg ctggaacagg atgaagttga tggtctggtt
     8881 tccggtgctg ttcacactac cgcaaacacc atccgtccgc cgctgcagct gatcaaaact
     8941 gcaccgggca gctccctggt atcttccgtg ttcttcatgc tgctgccgga acaggtttac
     9001 gtttacggtg actgtgcgat caacccggat ccgaccgctg aacagctggc agaaatcgcg
     9061 attcagtccg ctgattccgc tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc
     9121 tactccaccg gtacttctgg tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt
     9181 ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta cgacgctgcg
     9241 gtaatggctg acgttgcgaa atccaaagcg ccgaactctc cggttgcagg tcgcgctacc
     9301 gtgttcatct tcccggatct gaacaccggt aacaccacct acaaagcggt acagcgttct
     9361 gccgacctga tctccatcgg gccgatgctg cagggtatgc gcaagccggt taacgacctg
     9421 tcccgtggcg cactggttga cgatatcgtc tacaccatcg cgctgactgc gattcagtct
     9481 gcacagcagc agtaatctcg tcatcatccg cagctttgcg ctgcggatat ctgaaccgga
     9541 aataatcact atttccggtt ttttattctc ttaatctgca ttaatccttt ctgattatct
     9601 tgcttaactg cgctgcatca atgaattgcg ccatttcact ttgcatactt accactttgt
     9661 tttgtgcaag ggaatatttg cgctatgtcc gcaaccactg aatccaaacc aacaagaaga
     9721 tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg ctattttaac cagccttgcc
     9781 acctgggtag ttccggtggg gatgtttgac agtcaggaag tgcagtatca ggttgatggt
     9841 caaacaaaaa cacgcaaagt cgtagatcca cactcatttc gcattctgac taacgaagca
     9901 ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg
     9961 atgaacttcc cgtttgaagg attaacctca ggatcgaaat acgggacagc tgttggcatc
    10021 atcatgttta tgctggtgat tggcggcgcg tttggcattg tgatgcgtac aggaaccatt
    10081 gataacggta tcctggcgct tattcgccat acccgcggga atgaaattct ctttattcct
    10141 gcgctgttta ttctgttttc acttggcggc gcggtatttg gtatgggaga agaggccgtc
    10201 gcctttgcca ttatcatcgc accgctaatg gtccggctgg gctatgacag tattaccacc
    10261 gtcctggtga cctatattgc cacgcaaatc ggttttgcca gttcgtggat gaacccgttt
    10321 tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc tttctggctc cgggttgcgc
    10381 atcgtggtgt gggttatcgc cactctgatt ggcctgatct ttaccatggt gtacgcctca
    10441 cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa
    10501 aagcaggctg atgttgaaca acgtccgttt acctttggtg actggctggt attgattgtc
    10561 ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca
    10621 gaaattgcca gccagttctt caccatgggt ctggtgattg gcatcatcgg cgttgttttc
    10681 cgccttaacg gcatgacggt taataccatg gcttcatcct ttaccgaagg ggcgcgaatg
    10741 atgatcgccc ctgccctgct ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat
    10801 ggtgaagcag gtgatgccag cgtgttaaat accatcctca acagcattgc caatgccatt
    10861 agcggtctgg acaacgctgt cgcggcctgg tttatgttgc tcttccaggc ggtatttaat
    10921 ttctttgtga catccggttc tggtcaggcg gcgttaacca tgccgttact ggcaccgctt
    10981 ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg cattccagtt tggtgatggc
    11041 ttcagccaca tcatttaccc aacctcagct tcgttaatgg cgacgctcgg tgtttgccgg
    11101 gtggacttcc gtaactggct gaaggtgggc gcgacactgc ttggactgct gtttattatg
    11161 tccagcgtcg tggtgatcgg cgctcagttg atgggctacc actaaaaatg ttaagagccg
    11221 caattgcggc tcttttttca ttctgccgtt tcagtctcta cggcttcatt tttggcattg
    11281 cgcttcatcc acaacgccag cgctttcagc gagtctggag tgaactcatc gcagcgtgcg
    11341 gtgatttctt ccggcgtcag ccagcaaact tcactgactt catcttcctg tagggcgaag
    11401 ggaccgtgag agacgcagct gaacaatgcg ccccagacac ggcaattttt atcttcgaaa
    11461 tagaactgcc cgtgctcagc aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg
    11521 cgacgcgcgg attccagcag ttgctcatcg gcctggacca caccgcctgc ggtcgcatct
    11581 aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat tttgcccatg
    11641 ccatcatgca cgacgatgta agttgcacga tgacgcagac actgtgcccg catttgttcc
    11701 cggctggctt gtgcaatgac ttcgttctct tcattgacaa tatccaccca ttcagtactt
    11761 gccaaacgac gctgttccat tatcaggaaa ccttcttttt ctggcgctct tacggcgcat
    11821 tttgagttgt gggtaaatta cggattaatc gcgacctgcg caatgatact ttgatcattg
    11881 agtgcgatta cactaagtac gtcattatcc agcatgccat aactcgccgg attaccgcct
    11941 ttcggaatac tcaccgagcc ggggttgaag tggaaaatct ccccccgctg ttctgccact
    12001 ggcagatggg tatggccgta caccagcaca tcgttctggt ttaaagcagg cagattttcc
    12061 gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc
    12121 cacggcgcgg ttatcgggaa atgcagcagc atttgatcca cttcgctgtc gcagttgccg
    12181 cgcacagcga taaccttatg tgccacttca ttaagccgtt cagcgacttt ggctggcgcg
    12241 taaccctccg gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag gatcaccagc
    12301 cactgggcac cgctttgggc aaacaactcc agaacacgtt ccgtcgccgg taacgaccca
    12361 tgaatgtccg atgcaaacat cagtttcatc actcactcct cgtcgaaaaa acgtaagcta
    12421 tgatactgga ttctgtcgtt gctatcagcc agattgcttc gccgaaagtg caataaaacg
    12481 ctggacagac gctcgctgcc actggaatgt cgcataatcc accaggcgtt ctggcacctc
    12541 atcgccatgt agtaccaggc ggttaatcat tagcgccaga tcagtatcag caatgcacca
    12601 ttcaccaaat aaattcggct gacccagtac taacaaatgt tctgccatcg caaacagttt
    12661 ctctgcactg gcttttccct cggccgttag tggcgctttt ttcgcccccg caaagacaac
    12721 atccgtcgga cgctcttcgc ggatgggcat cagatcgctg cgcagccagg cctgaatctg
    12781 tcgtgcacgc gcacgatttt ctaaatcaag cggataaata cgttcccagg tcggtggcgc
    12841 aaatcgatct tccagatact ccgcaatggc agaagattca ctcaactcaa aatcatcgat
    12901 ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc cacgtcggct gcaaatgttc
    12961 accgctgtcg aggtcgatgg tcttgatatg aaacgacagg cctttttcct gcaacgccac
    13021 ccaggcggat aacacataag gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg
    13081 tttactcatt atgtcctcgg caataaaggg ggctggctat caaactatag agcctttttg
    13141 tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct ttctatactt gtggtgttca
    13201 gatgattaaa caaccggagc tgcaatgatc gatctctatt tcgccccgac acccaatggt
    13261 cacaaaatta cgctgtttct cgaagaagca gagcttgatt atcgcttgat taaggtagac
    13321 ctggggaaag gcggtcagtt tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt
    13381 ccggcaattg ttgatcattc tcctgccgat ggcggcgaac cgttaagcct ctttgagtct
    13441 ggtgccattt tgttgtatct ggctgagaaa acaggactct ttttgagtca tgaaacgcgt
    13501 gagcgcgccg ccacattaca gtggttattc tggcaggtag gcggactggg gccgatgctt
    13561 gggcaaaatc atcattttaa tcacgcagcc ccccaaacca ttccttacgc tattgaacgt
    13621 tatcaggttg aaactcagcg tctttaccat gtactgaaca agcggctgga aaactcgccc
    13681 tggctgggag gcgagaacta cagcattgcg gatattgcct gctggccgtg ggttaatgcc
    13741 tggactcgcc agcgaattga cctcgcaatg tatccggcag tcaagaactg gcatgagcgg
    13801 atccgttcgc gccctgccac cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt
    13861 tcggatagtt aacagaaaca ggttctcgtg tattatttca tcctaagtaa aacaacggag
    13921 aacctgcaat ggcacaacct gccgctatta ttcgtataaa gaaccttcgt ttgcgtacgt
    13981 ttatcggaat taaggaagaa gaaattaaca accgtcagga tattgttatc aatgtgacga
    14041 tccactaccc cgccgataaa gcgcgcacta gcgaagatat caacgatgcg ctgaattatc
    14101 gcaccgtaac gaaaaacatt attcagcatg tagagaataa ccgtttctct ttgctggaaa
    14161 aattaactca ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg tatgctgaag
    14221 tggagatcga taaactgcac gcgctgcgct acgccgattc ggtatccatg accttaagct
    14281 ggcagcgtta accgccatat cgggaggctg catgaacata gtgatcaccg gagggacggg
    14341 attaattggt cgccatttga ttccgcgttt gctggagctg ggccatcaaa tcacggtagt
    14401 gacgcgtaac ccacagaaag ccagttccgt tctcggccct cgggtgacgc tatggcaagg
    14461 gctagccgat caaagcaacc tcaacggcgt tgatgcggta atcaacctgg ccggagaacc
    14521 gattgctgat aaacgctgga ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa
    14581 tatcacgcaa aaactggtcg atttgattaa tgccagcgac acgccgccgt cggtactcat
    14641 ttccggctcg gcaacgggct attatggcga cttaggtgaa gtggtggtta ccgaagagga
    14701 accgccgcat aacgaattta ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg
    14761 ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga
    14821 cggcggtatt ctcggtaaaa tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg
    14881 ttccggtcgg cagtatctgg cctggattca tattgatgat atggtcaacg gcattctctg
    14941 gctgctggat aacgacctgc gcgggccatt taatatggtt tcgccctacc cggtacgcaa
    15001 tgaacaattt gcccatgcgc tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc
    15061 cgcaaccgcc attcggctgt taatgggcga atcttcaata ctggtattag gtggacaacg
    15121 cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt cgctggtacg atttagaaga
    15181 ggcgctggcg gatgtcgttc gctgatgtgg tttacagcaa acatccgcca gttaactccc
    15241 ggtgttacag gattagtggc tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat
    15301 cataactaac tctctgtcta aacctctatc cagcatctcc tgagcaatac gcagggcttc
    15361 ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg tcagcaatgg tcatcagttt
    15421 ctccttttct tgtggtgcgc gttctgttat ctcaccaata aatgcacgga aacgctgggc
    15481 atcccctgtt tgtaatacgt aattaaacag ggcttttagc tgtctgtcat tagtgttccc
    15541 tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct gacgaatatg
    15601 tttctgaatt aactccaaca gcgccatttt gcggtgttgc ataatctcgt catccggcac
    15661 cacggtaata tccaccaacg gaaacgctga tgaatatatt ttgcgggcta tagcaggctc
    15721 ggcaaattca tcaagccagc agagtgaata aggataagga cttctgcaac catgataaaa
    15781 cagcatcggg atcaccaatg gaagctcttt atagcccgca tcaagatggt tttgcattgc
    15841 cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac tttggtgctc
    15901 tatcactaca taaatataac ccactccctc ctgcgttttc acagaccaca agaggtcgga
    15961 ataatattgc cgcaggtcgt catcaataaa actgtttggt tccagtttaa gcgtcgttaa
    16021 atcacacagt ttgcgcagcg gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc
    16081 tggatggcgt aaaaaagatt taaataccgc atcatgcggc gtggaagttg tcgatattgt
    16141 catggcgttc cgtcacccct caaatttaag atgacgcgac aataaccacg attaacaccc
    16201 aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt agcggactgc
    16261 aacttttaca tttcagcgga agctgactca caaattcccc atgaaaatac tccggcagcg
    16321 ttagccaccg gagaggaaat tatttcagcg atcccttaag gaactgttgc agacgagggc
    16381 tttgcgggtt gccaaataac tgctccggcg cgccctcttc ttctattttt ccctgatgga
    16441 ggaaaatgac gtgagtagaa acatggcgag caaagcccat ttcgtgagtc actaccacca
    16501 tggttttccc ctcttctgcc agttgctgca taatacgtaa cacttcgcct accagttcag
    16561 gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc cggttccatc gccagcgccc
    16621 gcgcgataga aacacgctgt tgctgaccgc cggaaagatg caccggatat ttcccctgcg
    16681 cacgttcgtc tatcccgact ttcgccagat acttcaccgc ccgctcgcgc gcttcctgct
    16741 tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc gtcatatggc
    16801 tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat
    16861 ttttatcggc gactttgagt tgaccatctt tgtcgcgcac cagattgatc gtctggccat
    16921 tgaccacgat cgacccttca ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac
    16981 ttttccccga tcccgacgat ccgatgatgc ttattacatc tccggcattc gcttgcagtg
    17041 agaccccttt cagcacttca tgttcgccgt agcgtttgtg caaatcgata acgtttaatt
    17101 tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt
    17161 ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata gagcaccgcg
    17221 gcaatgccga aggcggtaaa aggttgatac gtggcggcgt taatatcgcg ggctattttc
    17281 agcagatccg gcacagtggc cgtaaacgcc agcgcagtag agtgcagcat caggatcact
    17341 tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag gcaaaataat gcaacgatac
    17401 attttaaaag tcgagaagcc ataggctctg gcggcttcaa tttccccatg cggaaccgaa
    17461 cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc
    17521 acggtacagt tcaggccact gcggaagaaa gcgttaagga attcggttcc cttaacaatc
    17581 tcaagcgtgt acatgccgga atagaacacc agcaactgaa catacagcgg cgtaccgcga
    17641 aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga gacacgacca
    17701 atcgccagaa acagcgccag gactccgcct atcactaccg acaaaataag cagccacaga
    17761 gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt ccagtattca
    17821 tgtaagattt cgatcacagg tcagccctct tcacacccac ggagtagcgg cgctcaagga
    17881 acagcagcac accattggaa acagtggtga aaaccaggta aatcacgcca cagacgatgg
    17941 cgaaatagaa cggttcccag gtacttttgc ctgccagttg ggtggctttg accacatctt
    18001 ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag ttgttgccaa
    18061 tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac acttgcccac
    18121 gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt cggcactgcc ataaacgcgc
    18181 cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtaata ataccagcga
    18241 ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg tttagtgcaa
    18301 tctgcaaacc gtagaaaatc agcagcatca acaccaagtc cggcacgcct cgaatcagcg
    18361 tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta ccgccagcgc
    18421 caattaaacc gatgattaca gcgagcacta cagaactgat agccagctcc agcgtgacga
    18481 gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc ctgtcgtgtg
    18541 tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgggc tttctttcac
    18601 cgatggcgat taaccgccat aaacatcaaa atcgaagtac tttttcgcta atttctcgta
    18661 agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc
    18721 ttctttacgc aggcccatgc cggtccctac gccaaacagt ttttcatctt taacagacgg
    18781 gccaccgaat ttgtaatctt taccgacagg ttgtttgagg aaaccttcgc tggcagcgac
    18841 ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg
    18901 cccctgatac gagacgattt caatgccttt tggtgcccaa tgttcattac cgaacgtctc
    18961 ctgggtggtg ccctgcaata cgcctacccg tttgcctttc agcgactcga ctgtcggctg
    19021 aatgtcggaa tttttcgcca ccaccaaacg agaatcggca gcgtacagtt tgtcggtgaa
    19081 ggctatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt
    19141 cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg tacattgcgt
    19201 attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca gttcgccttg
    19261 tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg
    19321 aatcgcagca aacgccgcag ttgcgctgga gaaggccaga accagagaga gcgatagcac
    19381 cagttttttc ataatgtcct caactgacag tctttttata gggaatattt acaggtttgt
    19441 tacacttatc gtgccataaa attgaccatc aaggcaaaat atcccgcccc gttagacgta
    19501 ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag tgcaacaaac
    19561 ctggcgtgca ccatgatgat cattccatca ggtacagctt cccagcgacg tatcagtcac
    19621 cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc gtaggtgccg tcctgacgca
    19681 gctcgccaag cgccttattg aaggcagccg tcagttcagc atcatcttta cgtagcccta
    19741 caccggtgcc atcaccgaag tattttttgt cttttactga tgagccagca aaggcgaaat
    19801 ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatct tgtaacgcag
    19861 catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca taggccacca
    19921 catccacgcc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt gatccctgca
    19981 gcacaccaac atgtttacct ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg
    20041 cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac
    20101 gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg
    20161 ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc cgtttgcaca
    20221 tctcgttacc gagatcgata tcaaagccaa caaaatcacc tttagcatct ttcgacgaga
    20281 acggcgcata ggtggtatcg gttccgatac gtaccgtctc cggtagcgcc gcatagctgg
    20341 aagccgctgc ggagagaccg actaacaaag acagagcgag aatcgacttc ttcatacata
    20401 accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt
    20461 tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa atatgttaat
    20521 aagacgttgc attattgtcc tgaagttgaa gatagcaggt atagcggttg aatcgcagcg
    20581 tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca aaattgccct
    20641 gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc ttcaggaagg
    20701 gttattgcaa actggtcaag aacacgatta accgtctgat ttatcacatc atcaagggat
    20761 tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat ttctgccgcc
    20821 tgagtcatta aacgcagatg gcctaagtgc aatggtgttt cacgcacgca gagcaccaac
    20881 gggcgacgct ctttcagcac cacatctgcc gcacgggtca gtaagccatc agtatagcta
    20941 tggacaatgc cggaaagggt tttgattgaa cagggtaaaa tcaccatccc cagcgtctgg
    21001 aaagaaccgg aagagatgct ggcggaaata tcgcgcgcat cgtgcgtgac atcagctaat
    21061 gcctgcactt cgcgcaggga aaaatccgtt tcgagggata aggtctggcg cgccgcctgg
    21121 ctcatcacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa taagcgcacg
    21181 ccataaatcg cgccgctggc accgctgatg cctacaatga gtcgtttcat aaatagttgc
    21241 cctgtcagac ttgcaggcag actttgcagt atttcggcgg gagttgcaag tcagggtgcc
    21301 agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc atttcgagat tttccacttc
    21361 gttctgacgt tgcactgctt tggcgtcatc attacgtaac gtatcgagga aatcgaggta
    21421 gccctgatca acatctttgg tgacgtagac gccgttgaac accgagcatt caaactgctg
    21481 gatatccgga ttttcagcgc gaacggcgtc gatcagatcg ttcagatcct ggaaaatcaa
    21541 cccgtcagca ccgatgatct ggcgaatttc atcaacttcg cgaccgtgag cgatcagttc
    21601 cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg gtgccgcaga
    21661 agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct gctcagaagt
    21721 ggtgccacgg acgatggagt cgtcgaccag caggacgttt ttatcgcgga actcggcgcg
    21781 gttggcattc agtttacggc gcacggactt acgacgcagc tgctggcccg gcatgataaa
    21841 ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggtttgc ccagaatacg
    21901 agcaatttcc agcgcgatat cacacgaggt ttctgggatc gggatcacca cgtcgatatc
    21961 cagatcttcc cattcgcggg caattttctc gcccagtttc gtgcccatat tcacacgcgc
    22021 gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata catactcaaa
    22081 caggcacgga ttgctgaccg gattgtcagc acattgacgg gtaaacaact gcccttcttc
    22141 agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc
    22201 gagcgctacg ctttcggaag cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt
    22261 tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa gcaaccatac cgtggccgat
    22321 aatcatcgcc acacaggcat atgcgccgcg gattaagcgg tttgtggcag caatggcagc
    22381 gaaaatattg tcggcttcca gcgggtagtg gcggaagttg tccagctcgc tagcgaagat
    22441 attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt cttcaaacag
    22501 ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg taatgccata
    22561 cggagagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag ccgtagggta
    22621 acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag cttcaaatac
    22681 atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat ctatggtgat
    22741 gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg
    22801 gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt ttcctcgtta
    22861 agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt gcagataatc
    22921 aaaaaaccat ctgatgataa aactgaattg cgggatcagc tgtgatttgc tccagtcttc
    22981 gcttttcgac accccggtaa aggagtcgag aaagaagaga atggcggcaa caatcaacac
    23041 accgcgcaac gcaccgaaac agacgcccag cacccgatcg gtgcctgaca accccgtttt
    23101 ctccaccaac tggcctatca cgaagttcac gatagcacca acgatcaggg tagcgataaa
    23161 cagtaccgcg atggcaattc catttcgaac cagttcgtct tcaaagcccg taaaccagac
    23221 tgacaggtaa gtgtagtaat gactggcaac aaagaaagca caaccccatg tcaccagcga
    23281 taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaaag caatcaccgc
    23341 gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc tgtcatcctg
    23401 ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat
    23461 caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc tttgcggcat
    23521 cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg acctacggtt ctaacactgc
    23581 ccaggcctga taagacgcgc cagcgtcgca tcaggcaaga ctgtattaat tcggcgtata
    23641 gcccattacc acgccactta agccagaaag ttgcttcaac tcacccagcg aacctttcag
    23701 cttatctttc gaggcatccg gcccaaccag aatacgggta attttaccct gcactggcgt
    23761 ggatggcgac gtataaaccc gataaccggc accgcgcagc ttaccgacaa tctcattcac
    23821 tttatcggca tttttcagcg cacccagttg cacaacatag gctttacccg ttggcgctgc
    23881 tttttcttcc acgaccggct tcggttctgg cttcggcgca ggtggtgctt caaccttcgg
    23941 tttaggcggc tccaccggtt tcgacttcgg tggggcgacc ggtgcaggtt ccggttcaaa
    24001 ctcggtgtta ttggctgcaa tagtggtcgg atcgagcgac ggtgctgcgg catcacctgc
    24061 ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc aacgcctggg tggcggctgg
    24121 catcatatca ggctcatcgc gatcgcccgc tttcggcacc agcgggatag ccgcgaactc
    24181 atcctgataa tgttttttct gcccgtccag cagccctgga agtacaatca cccccagcgc
    24241 caccagcacg atcgtgccca ctaaccgatt ctgaaactta cttgccaccg cttctcctcg
    24301 cgtcaatcac ttccatgaca tgtgcgacag tgtggaaaga accacacacc agcacggtgt
    24361 cttccgcttt agcgtccgcc attgcggcat cccatgcctg cgcaacgcta tcaaatgatt
    24421 tgccgttacc caaatgctca agcagttgtt ctgccgtggc accgcgcggc ccttccagtg
    24481 gcgcacaata ccagtcatca accacgcttt tcaaccaggc cagagttccg gcaatatctt
    24541 tatcatgtag cataccgata accgccagca cgcgcccgtt tttcggtagc gctttcatac
    24601 gcccggtgag atattccgcc gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg
    24661 actcgctcac aatctggaaa cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg
    24721 cattttcact gacttccagc ccgctggcac gcagtgccgc cagcgccgtt gcggcattcg
    24781 gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc
    24841 agtcatgatc ggtgacggaa tagttccact caacgccccg acgttgtaac agtgcacctt
    24901 tttcctgtgc cacatcagca atggtagaag gcatttccgg ctcaccgaca attgccggtt
    24961 tttcgctgcg gaagatgcct gctttctcgc gaccaatact ttcgcgatct ggacccagcc
    25021 agtcggtatg atccagcgca atactggtta ctaccgcgac atcggcatcg acaatattgg
    25081 ttgcgtccag acgaccgccc agccctactt ccagaatcac cacgtcaagt tgtgcctgct
    25141 tgaacagcca caacgccgac agcgtaccgt actcgaaata ggtcagggaa atatcaccgc
    25201 gtgccgattc aatctccgca aaagaggcgg tgtgggccga ttccggcaat tcctggccct
    25261 gcacacgtac gcgctcggta taacgcacca gatgcggtga actgtagacg cccactttgt
    25321 accctgccgc catcagaatc gactccagcg tacggcaggt ggtgcctttg ccattcgtac
    25381 ccgcaacggt aaacacaaat ggcgctggtt tcagaacgcc aagacgcgcc gcgacctggc
    25441 tcacgcgctc aaggccgaga tcgatagttt tactgtgcag gttttccaga taagaaagcc
    25501 acgaagccag aggcgacgcg gcttgaggag tgcgtttgat aatcatggta tccgctgatt
    25561 cgttacggtg agaatagcaa aagggcagag ccagtggccc tgcccttatc agttatcagg
    25621 cctcaggttc ctgatccggt accgggggta ccactacgcc ttcacgcggc gcttcaggat
    25681 tcggcgctgg cagattcatc aacttcgcca gaatgctcgc cagtttcagg cgcatttccg
    25741 gacgacggac gatcatgtcg atcgcgcctt tctcgatcag gaattcactg cgctggaatc
    25801 ctggcggcag tttttcgcga acggtctgtt cgataacacg cggaccggca aagccgatta
    25861 acgctttcgg ttcagcgatg ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc
    25921 ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc gcgctcctgc atttttgcca
    25981 gtgccgcaga ggttttcgcc atctgcatca gcgacatcag tgcttcctgc atacgtgcgc
    26041 caccagaggc ggagaagcag atcagcgggc agttatcttc cagcgcctgc tcaacggcac
    26101 gcacgaaacg tgcacccaca acagacccca ttgaaccgcc cataaaggcg aactcgaatg
    26161 ccgcagcgac aaccggcatt ccatacagag tgcctttcat caccaccagc gcatcttttt
    26221 cgccagtttc tttctgcgca gatgccagac ggtctttata cttcttggag tcacgaaact
    26281 tcagcacatc tttcggctca agctcgctac ccagctccac aaggcttcct tcatctaaca
    26341 ggctatgcag gcgattacgc gctgtcatac gcatgtgatg gtcacacttc ggacagacct
    26401 caagattacg ttccagctca gcgcggtata aaacctgacc gcagctatca cacttagtcc
    26461 acaccccttc aggaatgctc gccttgcggg tgggagtaat gttgctttta attcgttcaa
    26521 tccagctcat tagggacctt tctgtctgaa cctggttcga tgccagtttt atctttgggg
    26581 acgcataatg ccatttttgc ccccaacaga ccatgaatgt tgcacattaa aacataacag
    26641 cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg
    26701 tgccgcagcg cgtttgtgac gaataaattc gaaaacccca ggcaatattg aaacaaaaat
    26761 aatgccaaca atcatcagct taaggttgct ctgaatgaag gggagcgtgc cgaagaaata
    26821 acccgcgtag gtaaaaatca gtacccacaa cagtgcgccg atcacgttat aagcggcgaa
    26881 atgacggtac gacatgtggc ccattcccgc aacaaacggc gcgaacgttc tgacgatcgg
    26941 tacaaaacgg gcgagaataa tcgttttgcc gccatgtctc tcgtaaaact gatgagtctt
    27001 atcaagataa ctgcgacgaa agattttcga atccgggtta ctgaacagtt tgtcaccaaa
    27061 taaccgtcca atggtatagt tcactgcatc accgacaata gcggcaatca acataagcac
    27121 caccatcata tggacattga gatcgttagt ttccagcgac gccaatgcac cagcaacaaa
    27181 cagcagcgaa tcaccaggca ggaatggcgt taccaccagc cccgtttcgc agaacagaat
    27241 caaaaacaga atggcataaa cccagacgcc gtactccgcg accagctctg ccagatgcac
    27301 gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg tccatatttg cctatgcctt
    27361 gtactcgtca tctttcaggc tgtaactacg ttggctacag cctgaaatgt tccgagcatt
    27421 attctcgtta gtccgccaga aatagcgggc ccattggcgg ttttggaaga tcataccggt
    27481 cagggtaatc caccgcgacc agatacagcc cttccgcttt tgccgttgct gccgcccgcg
    27541 ttctgtcctt tgccgccagc aactctgcta tccagctctc cggctggttg tgggcaccga
    27601 cttccatcag gctgccgaca atattcctga ccatatgatg tacaaaggca ttcgctttga
    27661 tatctaccac cacataagga ccgtgacgca tgacattaat atgcataacg ttgcgccacg
    27721 gggttcggga ctggcactgc accgcacgga acgaggtgaa atcattctcg cccagcaagc
    27781 attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt
    27841 tactcagtac cgccgggcgc agccgatgat tgtagatgat gtagcgataa cggcgagccg
    27901 tggcgctaaa tcgggcatga aaatcatcag gtacagcttt aacccaacgc acggcgatgt
    27961 caccaggtaa attcgcattt acgcccaagg tccacgccgc gtctttgcgc tgcgcggttg
    28021 tttcgaaatg caccacttgc ccggtaccgt gtacccctgc gtcagtacgc ccggcgcaga
    28081 agacggtgat gggttcgttc gccacctggg agagcgcctt ttccagcttc tcctgcacac
    28141 tgcggacttc attctgccgt tgccagccgt aatacttact gccgtcgtac tcaatgccca
    28201 gcgcaatttt ataaactggc agttgttgct ggtcggacat tagtacagat actcctgcac
    28261 cagtttctcg gcgattttta ctgccatcag cgcgccgcca aagcgaacgt tatcggccac
    28321 cgaccagaac tggacttgct ccggcatacc gtagtcatta cgcacgcagc caacagaaag
    28381 atgcggcgta cccgaagcat cccctacctg agtcgggaat tcgttctctt cagagagcac
    28441 aatatcttcg ccctgagcaa acgcatcacg cgcttcttct gccgccagcg gacgcagtgc
    28501 ttcaaagttg accatctggg catgaccgta gaataccggt gcctggacga cgcttgccga
    28561 aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg tcaacgatac gacgttcttc
    28621 acgcacgcta ccttcgctat ccggcaacaa cggcagcata ttgaacgcca gttgacgacc
    28681 gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat ttcgcactct gccccgctaa
    28741 cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc aggctggtaa cactgatacg
    28801 cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc agcagctggc tggtcagact
    28861 gtctggtacg gcgatgacat tccggttgcg gtaatcggtc agtacaaacg ggtttacttc
    28921 cggcaccacc agcggtacgt cgggttcgag agcaaacagt ccgctgctgt cgatcaccag
    28981 gcaacctgag ttggtcgctt cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa
    29041 aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca tcctgcacgg tgattgtttt
    29101 accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc
    29161 aaccgggaac tgacgttcag ccagcgtttc aagcagggct tcgcccacag cgccagttgc
    29221 gcccaggacg gcaatgttcc agccttcaga catggtggtt tactccagaa atagcaaagc
    29281 tccctgccaa tgttacagca gagagcatga agaagagatt aacgtgccgg atgatgaacg
    29341 gcgttaaaac ccagtttaca cagcaatgat gccgcactgg cgtcatcaca aattacatac
    29401 agagatgacc attcacggcg ctcaagatag tttttgcgca gtttatcgaa ctcacccggt
    29461 atcccggcga ctttacgcag cggtgcgtca tcgcggcgca catcatacac caaatgcacc
    29521 agccttttca gcgtcggttg atcgagcggg ccatgcagcg taatgcgacc aaactctggc
    29581 gcgggcagta atgtatccag cgcaacgtgc tgttcatgcc caataaactt gctataggct
    29641 tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg tataacctgc gatatgcggc
    29701 gtgccgatat ccacttttgt cagcaactcc acgttaagtt ccggttcgcc ttcccagaca
    29761 tccagcacta cgcttaactt ctggccttca ttcaggcagg tcagcaacgc agtattatcg
    29821 acgactgcgc cacggcaggc gttaatcaga atcgctcccg gcttcaggct acggatcagt
    29881 ttttcatccg ccagatgcag cgttttgtac ggcccatctt tgaagagtgg cgtatggaaa
    29941 gtcagaatat ccgcatgctg aactaattca tccagcgagc ggaaatcacc ctcatcccca
    30001 cggtcagcgc gaggcggatc gcaaagcaat gttttaatcc ctaacgcttc cagtcgcgcc
    30061 tgcaatcgac gtccaacgtt accaacgccc acgatcccca ccgtacggtc gtgcagtgaa
    30121 aatccatcgc gttcggcaag catcagtagt gaggagaaaa catattccac caccgcaatc
    30181 gcattacagc caggtgcagc ggaaaaacca attcccgcct gcttcaacca tgcttcatcg
    30241 acatggtcgg tccccgcagt ggcagtgcca acaaatttaa tgggttttcc tgccagcaaa
    30301 gattcattca ctttcgtgac cgaacgcacc atcagcgcat ccgcgtctgc cagttgagca
    30361 acggggattg gacgcccggg aaccgcggtc acctcaccca aacggctaaa taagtcgcgg
    30421 gcataaggca tattttcatc aacaaggatt ttcacgtttg tgttacctgt atgagacgag
    30481 agttaaccgg acaagtgtgc cataatctcg cggccaggca tacttgcgaa gatttcaggt
    30541 ataaggatac gtaatgatac aacctatttc cggccctcct cctgggcaac caccaggtca
    30601 gggagataac ctgccgtctg gcgcgggcaa tcagccttta tccagtcagc aacgtacttc
    30661 gctggaaagc ttaatgacga aagtgacctc actgacgcaa caacaaagag cagaactgtg
    30721 ggcgggtatc aggcacgata ttggtctgtc gggagattca ccgctgcttt cgcgtcactt
    30781 ccctgccgct gagcataatc tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc
    30841 ccgccagctt ttagcgcaat taggggagta tttacgtctg gggaataatc gtcaggcggt
    30901 cacggattat atccgtcata actttggtca gacgccgctg aatcagctct caccggagca
    30961 attaaaaacc attctcaccc tgttgcagga agggaagatg gttattccgc aaccacagca
    31021 gcgcgaggcg accgaccgtc ctttattacc ggcggagcac aatgcgctaa aacagctggt
    31081 gaccaaactt gcggcggcaa cgggggaacc cagcaaacag atctggcaat cgatgctgga
    31141 actttccggg gtgaaagatg gcgagttaat tccagcgaaa ctgtttaacc atctggtgac
    31201 ctggttgcag gcgcgccaga cgttaagcca gcaaaatacg ccgacgctgg aatcactaca
    31261 gatggcgcta aaacaacctt tagatgccag tgaactggcg gcgttatcgg catatatcca
    31321 gcaaaaatat ggcctttctg cgcaatcatc gctttcttct gcccaggccg aggatattct
    31381 taatcagctt tatcaacggc gggttaaagg gattgatccg cgtgatatgc aaccgctgct
    31441 taatcctttt ccaccgatga tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg
    31501 gatactgtta gtcgcgatta tcctgatgct ggtctggctg gttcgttagc cccgacgaaa
    31561 tgacagtatc gtgacaataa ttcccagcac cgcagagatc gccccggcaa gaaataccga
    31621 agagtaacca aacgtggtcg ccagcattcc cgccagcggc ccggagacgc cgagggcgat
    31681 atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga acttgtgagg ggacgcgttt
    31741 aaccacctcc acgcccagcg caggaaagat aagcgaacat ccggctccgg ttaacgccgc
    31801 gcccgctaat gcgacccacg cacctggggc ttgccagagc agcaacaagc ccaccgtttc
    31861 tacaagcaga gagacaatcg ccactttcac gccgccaaaa cggtccggca tccagccaaa
    31921 catgacgcgc atcacgacaa atgcgccgcc aaacgcggta agagtaaagc ccgccatcgc
    31981 ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc
    32041 ttgtagtgcc agccctaacc ctggtttcca gataaacccg acaacgctcc acagcgatgg
    32101 acgttctccc gccagggccg gtactttgcg cactgtgccg ttacaggccc acgccagtaa
    32161 gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg taatggctat gaatcaacag
    32221 gccaagcgga gcaccaacag cgagggcacc gtaaatcgcc attccattcc atgacatcac
    32281 tttgccagag tgttttggcc ctacgatgcc taacccccag gtcagagcgc ctgtcagtaa
    32341 ctggctttca ccaaacccaa gaatcaaacg cccgatgacc aacagggcaa atttgaacgg
    32401 tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg ccagccagac cacaagctaa
    32461 catcccctga agcgccgaac gttttgcacc atattgatcg gccagtcgcc cggcatagcc
    32521 acgcgtcagc accgtagcca gaaactgaat cccgacggca atcccgacca tggtattgcc
    32581 atagcccaga tcatgatgaa caaacagcgg gataaccggc aacggcagcc ctacggtcat
    32641 gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa aaattggcag aagatcgtgt
    32701 ttcggtttgg cttacagcag tcatgcatta ctccagaatg cagcgcaagg cgaggagtat
    32761 ccccgtctca tctctctggt ttcagggtta cggtgcgttg gcaggattta acgcgtacgt
    32821 cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aacaggcggc gattgtcaat
    32881 gatgtgaaaa agggaaccat caggttccct tttgcgttag tgccggaggc cgcattagcg
    32941 cgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct actccgacta ctccggccta
    33001 ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg ccgaagccga
    33061 agctgttaga cataacggtg gtcagttcgc gatcggtcgt ttcggtcacg atgttcagac
    33121 ctgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag ccgtgttcca
    33181 gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagagag tgaccggtca
    33241 tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg atagctgcca
    33301 gttctttcac gtcgccaacc ggagtcgaag taccatgaga gttcaggtaa tcgattgggg
    33361 tatcaacgcc gtgcatcgcc atcttcatgc agcgtactgc gccttcgcca gacggagcaa
    33421 ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag atgtgagcac
    33481 cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg ccagcgataa
    33541 cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg tcgttgtatt
    33601 tagtagacag cgcacccatc gcgtcgaatt cgcaggccat ttcccagcac agctcttcgc
    33661 cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact gcgttaccga
    33721 tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca tgaattttaa
    33781 acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc acatacgggc
    33841 caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaacacc tggaaacgcg
    33901 gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg gttattctgg taagcttccg
    33961 gagaaaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa atggatgcgt
    34021 cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt tttacgttgc
    34081 cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg atccctgaac
    34141 gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg gaaacaatgc
    34201 ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac atagagtaag
    34261 tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc catttaatag
    34321 agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt aagacgagta
    34381 acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc tgagggtaca
    34441 cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct ggaagagacg
    34501 cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca tccacatcct
    34561 ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac gctatggcag
    34621 gcatttaatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt acatttcatt
    34681 agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca acactggccg
    34741 gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt gcccggttgc
    34801 catcgtttat tgctcgatga aggccgcgtg acgctggatt tatggtttgg cgatattaac
    34861 gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg gtttctggac
    34921 ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa cgccatggca
    34981 aggttggcgc gtccgggcgg cacgctggcg acatttacgt ctgccggttt tgtccgccgc
    35041 ggtttgcagg acgccggatt cacgatgcaa aaacgtaagg gctttgggcg caaacgggaa
    35101 atgctttgcg gggtgatgga acagacatta ccgctcccct gctccgcgcc gtggtttaac
    35161 cgcacgggca gcagcaaacg ggaagcggcg attatcggcg gtggtattgc cagcgcgttg
    35221 ttgtcgctgg cgctattacg gcgcggctgg caggtaacgc tttattgcgc ggatgaggcc
    35281 cccgcactgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt aagcaaacac
    35341 gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg gttttacgac
    35401 caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg ctgggatgag
    35461 aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga actggctgta
    35521 gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg cagcggcatt
    35581 acttatccgc aaggtggttg gctgtgccca gcagaactga cccgtaatgt gctggaactg
    35641 gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt atcccgtaag
    35701 gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag cgtagtggta
    35761 ctggcgaacg ggcatcaaat cagccgattc agccaaacgt cgactctccc ggtgtattcg
    35821 gttgccgggc aggtcagcca tattccgaca acgccggaac tggcagagct gaagcaggtg
    35881 ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca ttgtattggt
    35941 gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca gcagcagaat
    36001 cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt tgatgtcagt
    36061 gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc aatggtaggc
    36121 aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga gcagaaagat
    36181 gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt aggttctcgc
    36241 ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag cgacgaaccg
    36301 attccgatgg atgccagtac gctggcggcg ttaaacccga atcggttatg ggtgcggaaa
    36361 ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt tgccggatgc
    36421 ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac gcgataagcg
    36481 tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct gctgaaacag
    36541 gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac cggcaccaat
    36601 ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt ggtcatcacc
    36661 actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac tggcaaacaa
    36721 ctcatcatca ctggcatggt caaccatacc gtcgattaat gccagaatgc gtgattcaaa
    36781 ctccgcgatc atcttctttc ctcattgtaa aacgtggcac cggcttcagt tgagcgcttc
    36841 cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt gtagtgcttt
    36901 aatgaagcgt gccgggcgtt caggaatgcc gtttgctagg taatccatca cctgcgcatg
    36961 tacccgccgt tgaaacacca cacgatccgg ttcgaagtcg ccttccagat tgtcgcaact
    37021 gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgaacgcct gcggcttcac
    37081 ttcaacatct tcaaactggc tttgcgtttg ggcatcgcgt ccgtccgggc aataccagta
    37141 gccgaaatca accagctcac ggcgcgcttt cccagcaata caccagtgcg aaatctcatg
    37201 aattgcgctg gcataaaagc catgagcaaa gacgattcgg ttatacggta cttccgcatc
    37261 agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa attcatcggc
    37321 aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt tcattagttc
    37381 atccccaacc agtggaggat ctcctgtccg tggctatcaa aaagtaattt ggcactcatc
    37441 accgctgaga caataacaat catcgggcgg atcagctttt gtcctttgct caacaccagt
    37501 cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca tgacaaagcc cgtcgcccaa
    37561 atcactttgc caccgagaat aaacagcagt aaaccgccga tgtttgacgt tgcgttgagt
    37621 aatttggcgt gcgccgtggc tttggcgagg ttgaatccgc acagcgtaac aaaggccagt
    37681 gcgtaaaacg atccggctgc cggaccaaag aatccgtcat aaaaaccgac gcagccaccg
    37741 gcaattaacg cgaagggtaa accgtacatc cgacgctggc gatcttcttc acccagcttt
    37801 ggcatcagca agaaatagag gccgatacaa attaccagaa tgggcaaaat ctgccgtaag
    37861 acatcagcct gaacgtattg caccagcagt gcgccgctca ttgagccgac aaaggtcatg
    37921 gcgatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa gtagatagta
    37981 gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagcgcatt agcgggagac
    38041 atccccgctg ccatcaatgc cggaatggtg agtaacccac cgccaccggc aatcgagtcg
    38101 ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg ggaaaccata
    38161 aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc tggcaggaag
    38221 gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt ggaggttcaa
    38281 accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt tgatcttcac
    38341 actccagact atcggcagga caacgtaatc gtacatgcat atgcgcacga tgctggaacc
    38401 agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atctaggcaa agttgttgtt
    38461 taatcgccgg attaacaaaa atgcgcgtga cgtttttgtc ctgggcggcg agtttgatca
    38521 agctgaaaat ttctggcttc cacagcgagg agacaacgtg tttaccgtcg cgggaaacta
    38581 agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc ggcagttgca
    38641 gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg aaacgcccac
    38701 cagcaggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg ctcacctggc
    38761 tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga cgctgatcgg
    38821 tacgcatgac ctgataatgt tcggactgta tcggcagcgt gtcagcgccg acaatacagc
    38881 cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt attttttgcc
    38941 acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca atcgcggttt
    39001 tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc gcccgctgcc
    39061 gtaacaggtg atccattaaa acgatcgcca gcatcgcttc tgcgatcggc actgcacgga
    39121 tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg ccaaagcggt
    39181 taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata tgggcaatga
    39241 tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc tggaaaccgt
    39301 ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca aaaccgtcgc
    39361 caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg tcggcatcca
    39421 ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc actggcaaca acggtgactt
    39481 tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct aacgcgtcga
    39541 ttttgtccgg gtccgggcag aagaacggat tttgttcaac cagcgaccag tctttgattt
    39601 ccagcggaat gtcgcccatc tgggtcaagc agccgcggat ttcaatacca aatttctcgg
    39661 cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg cgggcggaag
    39721 aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg taatcagcat
    39781 ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg agagcgctgg tcagtgtttt
    39841 cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg gagagaattt
    39901 tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg cgacgacggt
    39961 cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca ccatcgacga
    40021 tgcagccgag cgccagccca tgcgattcgc cgaaggtggt tacgcgaaag agttgtccaa
    40081 ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac ttaatcttta
    40141 taaatcgcga aatattcacg ggccgccagc agctgctctt tggtgagcat aaacacacca
    40201 tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg ttccataaga
    40261 tgtaccatgc tgttgccgac ttcacaaatc agcacaccat catcagcaag gtaatctgcc
    40321 gcgttaccga gaatgcgacg cgtcagtttc aggccgtcgg tgccagatgc caggcccagt
    40381 tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc gacatacggc
    40441 gggttagtga caatcagatc gtattgcact ttcggcaaat cacggaacag atcggaacga
    40501 atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc aaccgccagc
    40561 gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata agcacaggca
    40621 atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg cttgctgatg
    40681 agtccggcaa atttattgtt gatcagttca ccaatcggtg agcgcggcac cagcacgcgt
    40741 tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta agccaccgga
    40801 atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc gctggaggtc
    40861 agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga tggtaacacc
    40921 agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat atttgccgcg
    40981 ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc atttactgct
    41041 tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata atttcggcgg
    41101 ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg aggaaaaata
    41161 cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt agaaatagag
    41221 acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg tttcgccagt
    41281 tgatggcggg gactcgcaag attaagcagg acacgattgt ccaccgaccg cagcgtaaaa
    41341 aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc agccattatt
    41401 tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat gttcgcccgg
    41461 atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg gaattgtttt
    41521 tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg ttgattgccg
    41581 cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg aagcatattt
    41641 tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca tttcaccagg
    41701 caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg gaagagtggt
    41761 tgccgccgga gttgccctga ggagttgagc gtgttgtgcc aggagcgcgg aatggcaggg
    41821 gcgaatgacc ctgccaggtg agaaaaggtg cgctaataca tattcagtat gtaagtgaat
    41881 cggcaggtag aatctatggg ctgtcgagat gtcgtgtcca cgcgtagctt catatcatgc
    41941 agttgcctgg acgtgatcgg ttgcggctct ggcgtcaccg tcactttggc attatcacag
    42001 ccggaagtcg agaaggtcat ctgctgtgcg acaaaacggc agtaggctcc acgctcgtca
    42061 aaattactgc catcacacag tttgccgttt tccatcagga aagtgggcag tgtttgctca
    42121 ataacaccac ctgttgactg caacctgacg actttgttat gtacaacata attttcattc
    42181 caggaaacat catacggtgc cagggactct gtcgggcagt cagagctaac ttttgttggt
    42241 cctataaaaa ttccggcccc gaaacgctga tccatagtac tggcgctttc aacataagac
    42301 gacacaatcc agggacaacc gtgtaaatct ccatcgtaat aaacgccaag tactaattca
    42361 ttacatgacc aggccgttac tggagagaga gcatcatact cttctaatcc ggggcaggta
    42421 gaatccggta aggttgtatt attgcgctcc atcctgagtt cagggccggg aggactcccg
    42481 gtccaactaa ctaaaggcaa acttgctgcc gcagttgaat tagattcata cgtgccatat
    42541 tcaactgatt gaggggtcag gactgaaaga tgaaaacgat actttgcaac aacgctggtc
    42601 gtccccgcgg gtacatagag tgtggcgatc tttgtcgcag cctgtatccc agaagcgcac
    42661 agagccagca ccaataaaca tataacatga cgtattttat gcattttata tatactcaaa
    42721 cgtagctgtc aggctggcgg tgaaatcacc gatggttaca tcacgaccgc ttttcgcctg
    42781 gatccaggcc cggaaattaa tatcattgtt gccgttggta agcgcaaaag tcgccccggt
    42841 ggtgttgtta atatccaccc gcttgccgtc gagcgtttcc atccctatcc ccacgccctg
    42901 cgcggtggag gtgtcgtcga ctgccagaaa acccggctga ccgctgtctt ccgttccggt
    42961 gagggtgact ttgacgttgt atcccgccgg ccctttgcag tctttcagct taaacacaac
    43021 cggcagatgc gcggactgcc cggcgaccat caaatcccgg ttgctgacgg tgggaaaatg
    43081 aatttccgcc agattggcac catccaccac cagcgtgcag gatttactca gcaggttgcc
    43141 gtaaaaatgc aggttgttat cgactgcccg tgcccccgtg ctggcggtca gcagcaggcc
    43201 tgctaacagc gcataaccgg gtgaataaat gtgtgctgtt ttcattggta atccacccgt
    43261 aaggttgcgt aagcagaaaa attcgtgtcc gtcagcaccg catcagccgc tttgaccggt
    43321 accgcctcca gctttggctt ctggctaaaa tcggtggcgt taatggaaag cggctcgttg
    43381 agcttaaaac gctgaccgtc atgctgaagc tcaattccga agcccggtac atccgtctca
    43441 atagcagagt cgttgtagga ggtggcagtc cccgtccagg agagtgtcat attccactcg
    43501 ggatcgcgga catccgagtc gcagctcagt tcgtagtcca ctttctgacg accataattg
    43561 ccatcaatgc tgtcgataat aagatcgcga aattgcactt cgatggtttg cccgccgctg
    43621 atgctacagg cgggcggcga catcagcgta ccgtggaatg tgatgtcatc ttccgccgcc
    43681 agcacagccc cgcaaccagc gcacagtaaa aggagcagga attttttcat tggtaatcca
    43741 ccaccatggt catcgtggcg ttaaactccg acggcgtcag ttgcaccccg ctctgtttta
    43801 ccggcaccgc tttcagctga ggctgagtgt tgacattaaa aggcgtccag ttattttcac
    43861 caactgcaaa cagggagttg tcggcggcgt tttctatccg cacccccagg ccggcgatat
    43921 ccgtgctgag aaccgtttct ccgttgatca cggcggtatt gcctttgagc tgcatccgta
    43981 agtcatcgct gactttatta ccgcagttga gggtgtattt cgcatcctgt tggtaattgg
    44041 taccatcgat cctgttgatt atcacattgc caaatttcac agcgcttccc ttcacggagc
    44101 aaggcggcgg tccatcgacc aggattcgca gcgtcagatc cacctgtttg atatcactgc
    44161 cagcaacagc caaaccgctg atgccccatc cggccagcac cagcaaccct gcgcccgcta
    44221 caaacttgtg actcataagt ccctcccggc atcagccttt gcggctctgc tcttcatcca
    44281 cctggcagct gttgccggtg catatgaagt acagcggcat ccgcgcgccg tagtcgttga
    44341 tgtaagtcag caccggcacg ctgtccatct tcgcattcag cggtgcggag gttttcggct
    44401 ccatcatcag cggtgtgaat cccgccggcg ggttgccgcc ttttttagcg ctggcgttgc
    44461 tgatgatcac gtagtacggc gtcgggttat tgacggtgaa gtcctggccg ttacggatga
    44521 gcgtcacctt atgctgccac ggacttttca tgctggtgtt ctccagcgcc ttcggtcgcc
    44581 agaacagttt gatacgcgtc tgcagcgcaa tctgcagtgt gttcggttta tttgatttcg
    44641 gcggaatttc gcgcacgttg aaatagaaca cgctctcgcg gtcggccggc agtttgttga
    44701 tatccggcag cccctgcacc ttcacctggc cgttcatcat cgcatcgata cgctgcactg
    44761 gcggaagcac cgcaagcggc gagctgattt tgttgccctt tgcatcctct atccagctct
    44821 gcgccagata aggcagtgtg ggatcgttgt tgcgcagcgt gacgctgatg gacttgtcgc
    44881 tttcgttaaa caccagccgt gtcctgtcgg gcgtcaccga agcgtggctg gagaaactga
    44941 ccactgtcgc cagcagcgcc agtgccggga caacgatttt tgcagtgtta gtcagattaa
    45001 acatggatca ttccttaatc acgttactgg tttactgaaa ttgaagtcgg aggttccgtt
    45061 ggcgtcaccc gctgtgtctg ttcctgaatc gccggtttga ccgccggcga agtgataggt
    45121 gctacattgc ctttatgctg gcatggcagt aacaagccgt taaacagatc ggcaggcagc
    45181 gggtccggca ggttgatatc gcaatgcgca acaccactcc agaacacact catgtgttcg
    45241 ccaggtttca ccccagccag ataaacattg ccgtcatcat cgacaaggcc cactgtctgc
    45301 tcgttatcat tttttacttc tgcgccaaac ggaggatggc tgccgtcgct caggcgcagc
    45361 accgccatcg ctttttgtcc gctaatgaca gcaaatttac ggtagccaat ggcaccttca
    45421 gttagcgtgg cttgcaccac cgactgggtt gcttcagcgt tttcaggcaa tctgttgagg
    45481 tcgatatacg cctgattgcg gtaatagtta ttgacgtcag aaacgacggc tttaccaaac
    45541 atattggtat aaacagcagc cccgttacct tccaccggaa catcggcaac gccatcggca
    45601 tcaatcaaca atcgtgtccc gcccatattc tgggtacggt gaagtgcgcc accgtgggca
    45661 gtaagcgttg cgccgccctg taacgacaag cccgcagagg tgtattgccc ttcatggtag
    45721 ttcgcactga ggtcaacctg cgccagcgaa ccatcatggc tgtaatagcc gtcaacgctg
    45781 gtgtgtttgt cactggtgcc gacgttcaac tgatagtgag tcgcgtcatc gacacggctg
    45841 aaataaccga cctgactgct gtccgtccca ctgccatagt taccgttata gctaacggta
    45901 ctgttgtcgc cccacggcat actgagcgaa atgtacatgc ctttgtcggc ctggttgtca
    45961 tactcgtaac ggtagccagt catcgagatg ctgacattac gaatactgcc catattgaaa
    46021 tagtgcgaga gcatgatgtt gtagtttgtc tgctcctcgc gatcccagta ggtatggcgg
    46081 gtatagttga gataaaccga aacacccgca tcgcggaagt tctggttata agtggcggtg
    46141 tacatctctt tgtcgttgcc cgtgcgtacc attccgctgt cactggcatc cagatactcg
    46201 ctcatggtca taaagttctc ttccgagaag cgatatccag caaaagtaac gcggctgttg
    46261 agctggtcaa agtctttgga ataactcaca cggaaggagt taccgtccag cgaacctttg
    46321 ccataagcgg tgtctttatc cagtttggtg tgcgagtgag taacatcaaa cgcgaccgcg
    46381 ccgaatgtag acaaatcgcg accgacgcca agcgccgcag actgatagtt ttcatctccc
    46441 agtgcgccgc catataacga ccagccgtta gcgatccccc aggaagcttc agcaccagaa
    46501 aaaaattcac cctcgatatg gtgtccccac tcttgcggac ggcccatcat gatcttatag
    46561 cgaacctgac ctgggcgagt gaggtatggc atcgaggcgg tgctgatgtc atattcctgc
    46621 acctggccgt tctgttcttc aatgcgaata tgcaacgtac cggagacaga atcaccaaga
    46681 tcctgaatac gaaacggtcc agccggaacc tgcgtttcgt aaatcacacg ccccatctgg
    46741 ctgacggtca cttttgctgt ggtgtgtgcc acgccggaaa tgtctggcgc gtagccgcgc
    46801 agattgggag gcaacatttg atcgtcagta ctgacactgc caccaacata gttaaagcca
    46861 tcaaaaatat cggatctgag gtaatcctcg cccagcgcca gtttggcttt taatgatggt
    46921 aacgcccgcc aggcatagta gcgactccac tcccattttt tttgagtttc atcgccgctg
    46981 aattcatcgt catcatcatt actgcgagta tgttgatagt tagtctgcca gtcagcacgc
    47041 atacgccacg gccccaggtt aaccccgacc gtcccgttgc cgctgatctc gttactatca
    47101 tcaccgccat tttcttcgtg ccgggtttgt gcgttgatgc tgtagtccgc gacgatcccg
    47161 gagatgccgt catcccaacg tgaaggcgga tcccagtcgg gataagtata ttcgaggtaa
    47221 gcctgtggca gtgaaatgac taatgcggac tggcttaaat cagccttaat ttccatgcct
    47281 tccagttgac cggatttcag gcatttagca tcgtggctcc attgcagatt tttcgccacg
    47341 tcttctttta aaccaaactg cgctaccagt tccggtgtca gacaagcata gcttttgctc
    47401 gcgtcatctt caccagcata ccagtaaata tcgtactctt ccgccaatgg ctgtttattt
    47461 agttgaacct gtaaattgta ttttccgggc tcaacatatc cctggctgga aaaacgcttc
    47521 agatcaattt ttgtgtcgcc ttttaattcc agaaaacggg aatcaaactg aatgtcgtct
    47581 tcagcccaga cactgctata actccctgac attgccagcg caatgcacca cggaagaatc
    47641 cgtaatcgaa aaagagaatg gtcaggcata cacgtttcta tccgttttta ttcttgttcg
    47701 gtcaaaatcc atttaaatcc cccatagcag tgtcgctatg ggggtatgcc ttacaggtaa
    47761 gtaatctgga aggtggtatt tgcttcgaaa ttacccagat ctggcgcatc agctgcgccg
    47821 accagccagg ctttaaagtt cagtgtctgc ttcgctttgc ctttattggt agaagtgtct
    47881 ttcactattt tctggtcaat acccatgccg cttttgtaag aggtacccag agagtcaccg
    47941 atcgccaggc taacattgtt aaatgccgca ccggtatcgg tattgaaaat ggtgtaataa
    48001 ttgccgctat ttgcagaaga aacggtacca gtaaaggtag tggtcattgt ttcctgagta
    48061 tcaaatacgc aatcttgcag acgaatctgg aaacttttcg gcgtggtggt gccgttattt
    48121 ttcagttttt cggcaccgat atcacccata tcaacttcca gtttgtgatc gtccggaacg
    48181 atagaacaca ctgagtcttc aatggtgccg taaaaacgtg cagtaccgtt gttacccgca
    48241 gcgattgttg ccgtagaggt gaaaacgccc atcaccattg ccgcagcaat agctgtttt
//
LOCUS       DAZFJF010000018        46272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFJF010000018 DAZFJF010000000
VERSION     DAZFJF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.018"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /locus_tag="ACHKAU_001247"
     CDS             <1..231
                     /locus_tag="ACHKAU_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HFO5530354.1"
                     /translation="YSRFMMHLKYFSSRVLCNEKIKQKNIGDIYEQFLEKDFQLQRAI
                     HEIERYLYATFKYDSLLEEKLYLSIRIKVLMD"
     gene            263..1843
                     /locus_tag="ACHKAU_001248"
     CDS             263..1843
                     /locus_tag="ACHKAU_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO5530355.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            1843..3051
                     /locus_tag="ACHKAU_001249"
     CDS             1843..3051
                     /locus_tag="ACHKAU_001249"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO5530356.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            3020..3616
                     /locus_tag="ACHKAU_001250"
     CDS             3020..3616
                     /locus_tag="ACHKAU_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO5530357.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            3688..4635
                     /locus_tag="ACHKAU_001251"
     CDS             3688..4635
                     /locus_tag="ACHKAU_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5530358.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(4715..4791)
                     /locus_tag="ACHKAU_001252"
     tRNA            complement(4715..4791)
                     /locus_tag="ACHKAU_001252"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4755..4757),aa:Met,seq:cat)
     gene            5000..6097
                     /gene="mltA"
                     /locus_tag="ACHKAU_001253"
     CDS             5000..6097
                     /gene="mltA"
                     /locus_tag="ACHKAU_001253"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO5530359.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            6174..6980
                     /gene="tcdA"
                     /locus_tag="ACHKAU_001254"
     CDS             6174..6980
                     /gene="tcdA"
                     /locus_tag="ACHKAU_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO5530360.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(7031..7474)
                     /gene="csdE"
                     /locus_tag="ACHKAU_001255"
     CDS             complement(7031..7474)
                     /gene="csdE"
                     /locus_tag="ACHKAU_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO5530361.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(7474..8679)
                     /gene="csdA"
                     /locus_tag="ACHKAU_001256"
     CDS             complement(7474..8679)
                     /gene="csdA"
                     /locus_tag="ACHKAU_001256"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO5530362.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            8871..9098
                     /gene="ygdI"
                     /locus_tag="ACHKAU_001257"
     CDS             8871..9098
                     /gene="ygdI"
                     /locus_tag="ACHKAU_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO5530363.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9449..10366
                     /gene="gcvA"
                     /locus_tag="ACHKAU_001258"
     CDS             9449..10366
                     /gene="gcvA"
                     /locus_tag="ACHKAU_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO5530364.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            10385..10780
                     /gene="ygdD"
                     /locus_tag="ACHKAU_001259"
     CDS             10385..10780
                     /gene="ygdD"
                     /locus_tag="ACHKAU_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO5530365.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            10773..11873
                     /gene="rlmM"
                     /locus_tag="ACHKAU_001260"
     CDS             10773..11873
                     /gene="rlmM"
                     /locus_tag="ACHKAU_001260"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO5530366.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(11917..12648)
                     /gene="fucR"
                     /locus_tag="ACHKAU_001261"
     CDS             complement(11917..12648)
                     /gene="fucR"
                     /locus_tag="ACHKAU_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO5530367.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12706..13128)
                     /gene="fucU"
                     /locus_tag="ACHKAU_001262"
     CDS             complement(12706..13128)
                     /gene="fucU"
                     /locus_tag="ACHKAU_001262"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO5530368.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(13130..14548)
                     /gene="fucK"
                     /locus_tag="ACHKAU_001263"
     CDS             complement(13130..14548)
                     /gene="fucK"
                     /locus_tag="ACHKAU_001263"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO5530369.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(14657..16432)
                     /gene="fucI"
                     /locus_tag="ACHKAU_001264"
     CDS             complement(14657..16432)
                     /gene="fucI"
                     /locus_tag="ACHKAU_001264"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO5530370.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(16465..17781)
                     /gene="fucP"
                     /locus_tag="ACHKAU_001265"
     CDS             complement(16465..17781)
                     /gene="fucP"
                     /locus_tag="ACHKAU_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO5530371.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            18328..18975
                     /gene="fucA"
                     /locus_tag="ACHKAU_001266"
     CDS             18328..18975
                     /gene="fucA"
                     /locus_tag="ACHKAU_001266"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO5530372.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            19003..20151
                     /gene="fucO"
                     /locus_tag="ACHKAU_001267"
     CDS             19003..20151
                     /gene="fucO"
                     /locus_tag="ACHKAU_001267"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5530373.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(20206..20961)
                     /gene="xni"
                     /locus_tag="ACHKAU_001268"
     CDS             complement(20206..20961)
                     /gene="xni"
                     /locus_tag="ACHKAU_001268"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO5530374.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGI
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(21073..22440)
                     /gene="sdaB"
                     /locus_tag="ACHKAU_001269"
     CDS             complement(21073..22440)
                     /gene="sdaB"
                     /locus_tag="ACHKAU_001269"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO5530375.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(22498..23787)
                     /gene="sdaC"
                     /locus_tag="ACHKAU_001270"
     CDS             complement(22498..23787)
                     /gene="sdaC"
                     /locus_tag="ACHKAU_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO5530376.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(24344..25708)
                     /gene="ppnN"
                     /locus_tag="ACHKAU_001271"
     CDS             complement(24344..25708)
                     /gene="ppnN"
                     /locus_tag="ACHKAU_001271"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO5530377.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(25820..26668)
                     /gene="queF"
                     /locus_tag="ACHKAU_001272"
     CDS             complement(25820..26668)
                     /gene="queF"
                     /locus_tag="ACHKAU_001272"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO5530378.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            26736..27281
                     /gene="syd"
                     /locus_tag="ACHKAU_001273"
     CDS             26736..27281
                     /gene="syd"
                     /locus_tag="ACHKAU_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO5530379.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            27903..28232
                     /gene="yqcC"
                     /locus_tag="ACHKAU_001274"
     CDS             27903..28232
                     /gene="yqcC"
                     /locus_tag="ACHKAU_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO5530380.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            28232..29014
                     /gene="truC"
                     /locus_tag="ACHKAU_001275"
     CDS             28232..29014
                     /gene="truC"
                     /locus_tag="ACHKAU_001275"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO5530381.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            29032..29481
                     /gene="yqcA"
                     /locus_tag="ACHKAU_001276"
     CDS             29032..29481
                     /gene="yqcA"
                     /locus_tag="ACHKAU_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO5530382.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            29916..31268
                     /gene="gudP"
                     /locus_tag="ACHKAU_001277"
     CDS             29916..31268
                     /gene="gudP"
                     /locus_tag="ACHKAU_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO5530383.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            31270..32610
                     /gene="gudX"
                     /locus_tag="ACHKAU_001278"
     CDS             31270..32610
                     /gene="gudX"
                     /locus_tag="ACHKAU_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO5530384.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            32631..33971
                     /gene="gudD"
                     /locus_tag="ACHKAU_001279"
     CDS             32631..33971
                     /gene="gudD"
                     /locus_tag="ACHKAU_001279"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO5530385.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(34203..36959)
                     /gene="barA"
                     /locus_tag="ACHKAU_001280"
     CDS             complement(34203..36959)
                     /gene="barA"
                     /locus_tag="ACHKAU_001280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO5530386.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            37016..38317
                     /gene="rlmD"
                     /locus_tag="ACHKAU_001281"
     CDS             37016..38317
                     /gene="rlmD"
                     /locus_tag="ACHKAU_001281"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO5530387.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            38365..40599
                     /gene="relA"
                     /locus_tag="ACHKAU_001282"
     CDS             38365..40599
                     /gene="relA"
                     /locus_tag="ACHKAU_001282"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO5530388.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            40677..40925
                     /gene="mazE"
                     /locus_tag="ACHKAU_001283"
     CDS             40677..40925
                     /gene="mazE"
                     /locus_tag="ACHKAU_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO5530389.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            40925..41260
                     /gene="mazF"
                     /locus_tag="ACHKAU_001284"
     CDS             40925..41260
                     /gene="mazF"
                     /locus_tag="ACHKAU_001284"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO5530390.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            41331..42122
                     /gene="mazG"
                     /locus_tag="ACHKAU_001285"
     CDS             41331..42122
                     /gene="mazG"
                     /locus_tag="ACHKAU_001285"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO5530391.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            42350..43987
                     /gene="pyrG"
                     /locus_tag="ACHKAU_001286"
     CDS             42350..43987
                     /gene="pyrG"
                     /locus_tag="ACHKAU_001286"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO5530392.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            44075..45373
                     /gene="eno"
                     /locus_tag="ACHKAU_001287"
     CDS             44075..45373
                     /gene="eno"
                     /locus_tag="ACHKAU_001287"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO5530393.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(45433..>46272)
                     /gene="ygcG"
                     /locus_tag="ACHKAU_001288"
     CDS             complement(45433..>46272)
                     /gene="ygcG"
                     /locus_tag="ACHKAU_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5530394.1"
                     /translation="CSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLESIFNNT
                     QVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGL
                     EEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQYPTNPSESESAS
                     SSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDLFLFSDKIFSIA
                     VFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSSGGGGAS
                     GRW"
BASE COUNT        11634 a        10910 c        12117 g        11611 t
ORIGIN      
        1 tactccagat ttatgatgca ccttaaatat ttttctagtc gtgtgttatg taatgaaaaa
       61 ataaaacaga aaaatattgg tgatatctat gagcagtttc ttgaaaaaga ttttcagtta
      121 caacgtgcca ttcatgaaat tgaaagatat ctgtacgcaa cttttaaata tgattctctt
      181 ctggaagaga aactatatct ttctattcgc atcaaagtat taatggacta attatacata
      241 acattttcat gagataacca gtatgaaaca aaagaaagcc tggagttttt ttcagagcct
      301 ggggaaggca tttatgtatc ccatcgctct gttaagtgta tgtggtatga tgttagggct
      361 gggaagtggt ttagccagcg atgatatggg aaaattcatt ccagtgttgg ctattccgtt
      421 aattaaaacc atacttgatt ttattgttag ccttggtttg tttgccttcg ttaatttacc
      481 tgtattgttt gcgatagcta tacctttggg attattaaaa gagaaagatg ataaagccta
      541 tggcgctttt tctggcttaa ttggctttat ggcgatgcat cttggaacga acttttatct
      601 taaacagcac gacttattgg tagctgctga ccaaatgtcg acacatgggc aaaccatcat
      661 actcgggatc cagtcctaca ataccagcgt gttgggtggg attgtggcag ggttattagt
      721 cgccagcatg tataaaaaga tcgttaactt acgtattcct gaatcattag ggttttatag
      781 tggcccacgc cttgtgccta tcattacact gattgtgatg agtggatttg gtctgttcat
      841 tccttttatc tggccgccat ttttcaatct tttcatgctc attggtcact ggatttcaac
      901 ttccggtcct gttggttatt tcttctatgc tgttgccgaa cgcgtgacga taccttttgg
      961 cttaaatcac ttggtgacat cagttttccg ctttacccca atcggcggat cggcagtgat
     1021 tggcggtgaa gagtattacg gcaccctgaa catgtttatg gcatacgtca aagagaatgc
     1081 ggtcattccg ctggatttgg cggggaaaat ggagcagggc aaactgatga ttcagtatgg
     1141 tctggctgga gccgcgctgg cgatgtatcg cactgctcat gctcaaaaca gaagggctat
     1201 caaagcattg ctgatttccg gtgtgctcac ggtaattatt ggcggcgtca gcgagccgat
     1261 tgaatttctg ttcttatttg tcagcccgct gttgtttgtc ttccatgcct ttatgaatgg
     1321 attggctaac atggttctgc catatatggg cgtgaagatg ggctttactg gcgatctgat
     1381 tcaatttatt agctttggtg tcttacgtgg cacaagaacg ggatggccat tagcggtatg
     1441 tgttgaagtg gcttatttct tcatttatta ctttgtgttc cgttggacca ttctcaaatt
     1501 taacctgatg actgtaggcc gtgaagagtc cagtcctgtc acgctgaacg ctcacgaaga
     1561 aactgcaata gctaacatcc cagttcatga taaatcagag ttgcaggcag cggagcaaat
     1621 ggttaaggct ctgggtggta aagaaaatat taaatcactg gataattgtg taactcgttt
     1681 gcgtttaact atcgcggata tgggattgat agatgaggct gcaataaaaa gggccggagg
     1741 aattgcggtt gtgaaacttg atcaaaatac cttacaagtc attatcggta caaaagtcat
     1801 cgccctacgt cgggatatgg ataattacat ggggatatgt tgatggatat ttttaatacc
     1861 tcaatttctc gtaaaggaac atattgcaca caatgggact tttgtgaaga cagatttggt
     1921 gtaaaagatg tattaccatt ctctatttca gatatggatc ttccgatacc ggaagctatc
     1981 atcagaacat taaaaaaacg tctggaacac ccgattttag ggtacagtcg ttggcagcat
     2041 gatgactatc tgaattcgat agtcaattgg tatcatcaac aatataaaac agagattcaa
     2101 ccagaatgga tcacctatag cccaagcgtg atgtattcaa tatctaaggc aatagaaata
     2161 ttgacattga atggagataa tattctcatt ttcacgcctg tttataacgc attttttgat
     2221 gtgattaagc ataatggtcg aaacatgcta acaacctctt tgatgaaaga aaatgggaga
     2281 ggctacgtta tcaactggca agactttgag cttaaaatta aaagcgcgaa gatggtgtta
     2341 ttatgtaatc ctcataaccc gacaggaacg gtctggtcgg aagacgatct tcacagaatc
     2401 gcagcatgct gtgcaaggca taatatttgg ttatgttcag atgaaataca tagtgatttt
     2461 gtttttaatc atagtttcac gtcagcgcta acaataaaag atgacaaagt ggtggtgttt
     2521 aattcgattt cgaaaacatt taatgttcct gcgctaaccg gatcatatat gatttcaact
     2581 gacgataatt ttaatcataa gttcagaacg atatcgaggt atcgcgattt cgtaaactcc
     2641 ccatcagtac tgaatgtaat tgcgaccatt gttgcctata atgaatgtga agaatggtta
     2701 aagtcgttaa aggtacatat tgcctctaac attcaattta ccttgcagta tttaaatgaa
     2761 aatattccgg aattgatcgt atggccagct gagggctgct atttttcgtg ggttgattgc
     2821 tcggcccttg gttatccctt tgataaattt taccgtcgat taattaacga agggagagta
     2881 ggtattatgg cggggcatgt ttatggtact gaaggagagg gctatctgcg tcttaatgtg
     2941 gcctgcagtc gagaaaaatt atatatgggg cttgcgcgcc ttgcgagtgt aattaaaaat
     3001 attaatcagg aaaaagaaaa tgaacaatac ggatcttatc catcttatta aacgttttat
     3061 gcgtaacgaa cataaagcag tagaagaggt tattgattcc cccctttcgg aagtcgcgaa
     3121 tttaattaaa atattacaat cttgccaggg aaaagttgtt tttattggtg ttgggaaatc
     3181 tggcattatt gccagaaaac ttgctgcaac atttgccagc accggaactc cctcgttttt
     3241 tgttcacggt acggaagcgg tacacggcga ccttgggatg gtggcgaaag acgatgttgt
     3301 tattcttatt tcaaacagtg gtgagacggc ggaaatcctg gcgacactgc ctagtttgaa
     3361 aaaaatgggt aattatttga tctcttttac ccgcagtcat cactcatcgc ttgcgataag
     3421 ttgtgattta tctgttgaaa tccccgtcaa aagtgaggcg gataatttag gtttagcacc
     3481 gtcatgctcg tctactgttg ttctggttgt tggtgatgcg gtggcgcttg cactctctga
     3541 attgaaaaaa tttactcgtg ctgatttcgg cttatatcat ccaggaggtg cactcggcat
     3601 taaagcaaat tcataatttc aaagtataaa tacagtaacc ggtcacgcca tcttttgggg
     3661 tggcgttcat tgaagtgagt gtcagctatg aaaaatgttc tggtcaccgt ccccagcttt
     3721 tcagcacgct gtgtttccgc cagcaagttg ctaagagaga ataattttaa tcttattatt
     3781 aagaataatg tggagcatct gcttaaatca gaatccactg cattacgaga atctatttgc
     3841 gcggtcatcg ccggaaaaga ttgttatcag gcggacacgc tatcattgct gcctggtgtg
     3901 aaaataatat cgagattcgg gacggggata gataatattg atcttcgtgc agcacaacaa
     3961 tccggtattg tagtcaataa tgcggttggg attaattcaa atgctgtcgc ggaatttatc
     4021 attggactta tcttcgcgag catgagaaat attccaggca gctatcatgc gatgcaaaat
     4081 ggctactggg gtgagtcgca tggctatgaa ttacaaggca aacggattgg catggttggc
     4141 tacggcaata taggtaaaac cctggcaaaa aggttatccg gttttgatgt tgagctttta
     4201 gcttatgaca aacaacccga ttatcaggtt gctgacaaag ttggagttca gtttgtatca
     4261 attgaagata tctttatgca gtcgcatgtc atcattgttc ttttgccttt ttctcctgag
     4321 ctggagaact tcattagtca taaatatttg tcgatgatgc gcaatggtgc tctaatcatc
     4381 aatgccgcaa ggggaaagtt gctggatgag ggcgctttac tacaggtgat tgaagaacga
     4441 aatgtgtttg cggcgctgga tgtgtttagc tctgaaccgt tggcgcaatt tagcccactg
     4501 ctacatgcca aaaatatcat tacaacgccg catattgcgg ccgccaccgt tgagtcatac
     4561 cagcaaaccg gtacacacgt tgcccagtcg ataattgatt acttcgcagg aagggagata
     4621 aaaaacgtgt tgtgagttca gcgaacgaag tttttaaata caaaaaaaac cgcaatttgt
     4681 ggaacacatt gcggtttatc agcattctaa gtattggttg cgggggccgg atttgaaccg
     4741 acgaccttcg ggttatgagc ccgacgagct accaggctgc tccaccccgc gtccgtggat
     4801 gcgcactata ctccgctcgc gttttcatgc aacctttttt ttaccccgat cacggattta
     4861 gcatgatttt gaacaaaaac aaaccactaa agcctatttt ttgtgcaaaa tttgcgtcag
     4921 ggagaggctg ctcatttcat taaacgcggc ggtttgttat cttcgttgcg ccttattttt
     4981 taacctgaag aagagaacaa tgaaaggacg ttgggtaaag taccttctta tgggcacggt
     5041 tgtggcaatg cttgccgcct gctcttccaa accaaccgat cgcggacagc aatataaaga
     5101 cgggaaattt acccagcctt tctctctggt gaaccagcca gatgccgttg gcgcgccgat
     5161 taacgccggt gattttgccg agcaaattaa ccatatccgt aattcgtcac cgcgtctgta
     5221 tggcaaccag agtaatgttt ataacgcggt gcaagagtgg ctgcgcgcag gcggtgatac
     5281 ccgcaatatg cgccagttcg gcattgatgc ctggcagatg gaaggtgccg acaactatgg
     5341 taacgtgcag tttacaggct attacacgcc ggtaattcag gcgcgccata cccgccaggg
     5401 cgagttccag tatcctattt accgtatgcc gccaaaacgt ggtcgtctgc cgtctcgtgc
     5461 ggagatctac gcaggggcgc tgagtgataa atatattctc gcttacagca actccctgat
     5521 ggataacttc attatggatg tgcagggtag cgggtatatc gactttggtg atggcagtcc
     5581 gcttaacttt ttcagctatg cagggaaaaa cggtcatgcc tatcgcagca ttggtaaggt
     5641 gctgatcgac cgtggcgaag tgaaaaaaga agatatgtcg atgcaggcga ttcgtcattg
     5701 gggcgaaaca cacagtgaag ccgaggttcg cgagctgctg gaacagaacc cgtctttcgt
     5761 tttctttaaa ccgcaatctt ttgcaccggt gaaaggggca agtgcggtgc cgctggttgg
     5821 ccgtgcttct gttgcctccg atcgttcaat tattccgcca ggtactacct tgctggcaga
     5881 agtgccgctg ctggataata acggcaaatt taacggtcag tacgaattgc gtctgatggt
     5941 ggcgctggat gtcggtggcg caatcaaagg ccaacacttc gatatctatc aagggatcgg
     6001 gccggaagcc ggacatcgcg caggttggta caaccactat ggacgtgtct gggtgctgaa
     6061 aaccgccccg ggcgcaggta acgtctttag cggctgatgt gttattctgt ataccgtttt
     6121 tacggatacg acagggtgag ggataacctc acccttttta attctgaggt gctatgtctg
     6181 tggtaattag tgatgcatgg cgtcagcgtt ttggtggcac agcgcgtctg tatggtgaaa
     6241 aagcgttgca actgtttgct gacgcgcata tttgtgtggt tggtatcggt ggtgtcggtt
     6301 cctgggcggc ggaagcgctg gcgcgcacgg ggattggcgc aatcacgctt atcgatatgg
     6361 atgatgtgtg cgtcaccaat accaatcggc aaattcacgc tctgcgcgat aacgtcgggc
     6421 tggcaaaagc ggaagtcatg gcggagcgta ttcgccagat taacccggag tgccgtgtaa
     6481 cggtggtgga tgatttcgtg acgccggata acgtagcgca gtatatgagc gtggggtatt
     6541 cgtacgtgat tgatgccatt gatagtgtac ggcccaaagc ggcgctgatt gcttattgtc
     6601 ggcgcaataa aatcccgctg gtcacgaccg gtggcgcggg tgggcagatt gacccgacgc
     6661 agattcaggt taccgatctg gcgaaaacga ttcaggatcc gctggcggcg aagttgcgcg
     6721 agcgcctgaa aagcgatttt ggcgtagtga aaaacagtaa aggtaagctc ggcgtcgatt
     6781 gcgtgttttc tactgaagcg ctggtgtacc cgcagtcaga cggtactgtg tgtgcgatga
     6841 aagccacggc agaagggccg aagcggatgg attgtgcatc tggatttggc gcggcaacga
     6901 tggtgaccgc cacctttggg tttgttgcgg tttctcatgc gctgaagaag atgatggcga
     6961 aagcggcgcg tcagggttaa acaatcaggt cggatgcgac gcgaacgtct tatccgacct
     7021 ggttctgtgc ttaaacctgc ttcgtcgcag cgataatcgc ctcgcttaac gcattcaacc
     7081 cctggctgcg tgaggcgcta agctgcccac gcaatcccag ctcatcaaac aatgccagcg
     7141 gcgactgtgc ctgcaattcg gcggcggttt tcccctcaac ggcagtcaac agcaccgcca
     7201 gcaggccgcg cactatacgt ccttcgctgt cgccaaagaa gtgcatttta ccgttttcag
     7261 ccacggtata tcccagccag acgcggtttt cgcatccggc aatctcttta gcctgcgctt
     7321 ttaactcgtc tggcaaagcc ggaagctgtt tccccagcat gatcaactgg cgatatttat
     7381 cttcccattg cgtcaacggt gcgaaggtat tgcgtaacgt ttctgcggtt acggttgtgc
     7441 cgaacggatg tccggcgaat tgcgggttcg tcattaatcc accaataatt ccagcgcgcg
     7501 gtcaacggca ttcaccagcg catccacatc actctttgta ttatatggcg caaaagaggc
     7561 gcgcagtgtg ccggttacgc ctaattctgc cagtagcggc tgagcgcaat gttgcccggc
     7621 ccgcaaggca ataccgtact ccgccagcag cgtcaccata tcgctgtgat gaacgccagc
     7681 aaaatcaaag gccagcaggc tggaatcctg gcagcggaat gaacggaaac ctggacgttt
     7741 cgccagcgca tcttccgcca gtgttgctaa gctacggctc cagttttcgg cctggttgat
     7801 atcgtaatct gccagccatt ccagcgccgc gcttaatcct atgacaccag ccacatttgg
     7861 cgttcctgct tccagtttcc acggcgcaga ttgagtcgtg aagccgtcaa aactcacttc
     7921 gtgaaccatt ttgccgccgc ccagccaggg cgacatcgct tccagcagtt ctgatttgcc
     7981 atacagcacg ccgatacctg tcgggccata cagtttgtga cctgaaaaag catagaaatc
     8041 aatattcagt tgctgaacat ccgcggggaa atgcaccgcc ccctgagcac catcaaccat
     8101 caccaccatc ccggctgaat gagcaaaggt aatcgctcgc gccagatccg ggcaaccgcc
     8161 agtaacgttc gacatctgac ccaacgccag aatccgacta cggggagtaa tcagttctgg
     8221 caacaaatcg acatccggca gtcgctgcgc attaagcggc aatttcacca ctttggctcc
     8281 agtttgttgg gcgaccatca gccaggggac gaggttggcg tggtgttctg ccacgctgac
     8341 aataatctca tcgcccagtt gcagacgcgg acgcgcatag cattgtgcaa ccatgttgat
     8401 ggattcagtg gtgccgcgcg tccagacgat agttttatca tccggtgcat tcagtaattg
     8461 cgccactttc tctcgtgcag cttcataacg cgcggtcagg cgttgggctt cggcaaactg
     8521 gctgcgatgg acgtttccgg cgctcaggct gtaaaactgt cgggtggctt caaccacggc
     8581 ttcaggttta agcgcggtcg cggcgctgtc gagatagacg cccgcatcct gtagagcggg
     8641 aaactgggcg cgaaactgcg cgggattaaa aacgttcatg gtactcctcg gcttgatgca
     8701 ccgatcgtga cgcaaaattc actttgatac aaggagagtg ctaaccatca gaattatctg
     8761 gatatcctgg cgaagccgcg taagtaacat atgctgaata gtgttaggcg ttaattttag
     8821 cctgttaatc aatactatta agatttatta atcgctttaa ggagaagatg atgaaaaaga
     8881 ctgccgcaat tatttctgcc tgtatgctga cttttgccct gagcgcctgt tccggttcga
     8941 actatgtgat gcacaccaat gacggacgta ccatcgtctc tgacggcaaa ccacagactg
     9001 ataacgatac cggtatgatt tcgtataaag acgctaatgg caacaaacag cagatcaacc
     9061 gtactgacgt gaaagagatg gtcgaactgg atcagtaatt cacgatcgca aggtaaaaaa
     9121 aagcaccgca attaggcggt gctacattaa tcactatgga cagacagggt aaatgtacag
     9181 gaagtgaaaa aaggtagctt tgctaccatg gtctgaatcg cagaccaatt gcaaacacaa
     9241 caacacaaca tcacaaccgt aagccaaaag ttcaccagaa cacgcattcc gataaaactt
     9301 ttcgttccgg ctcaggaagt gccgccacta taggtatttg ctggtagaag ctcaacggac
     9361 aatttataat ggctcagatt aaaaaaacta ataggttaca cagtgtgatc taattgttaa
     9421 attcatttaa catcaaagtt taaaagccat gtctaaacga ttaccaccgc taaatgcctt
     9481 acgagttttt gatgccgcag cacgccattt aagtttcact cgcgcagcag aagagctttt
     9541 tgtgacccag gccgcagtaa gtcatcaaat caagtctctt gaggattttt tggggctaaa
     9601 actgttccgc cgccgtaatc gttcactcct gctgaccgag gaagggcaaa gctatttcct
     9661 cgatatcaaa gagatatttt cgcaattaac cgaagcgacg cgtaaactcc aggcccgtag
     9721 cgccaagggg gcgttgacgg tcagtttact ccccagtttc gccattcatt ggttggttcc
     9781 gcgactttcc agctttaatt cagcttatcc gggaattgac gttcgaatcc aggcggttga
     9841 tcgtcaggaa gataagctgg cggatgatgt tgatgtggcg atattttatg gtcggggcaa
     9901 ctggccgggg ctacgggtgg aaaaactgta cgccgaatat ttattgccgg tgtgttcgcc
     9961 gctactgctg acaggcgaaa aacccttgaa gaccccggaa gatctggcta aacatacgtt
    10021 attacatgat gcgtcacgcc gtgactggca gacatatacc cgacagttgg ggttaaatca
    10081 tatcaacgtt cagcaagggc caatttttag tcatagcgcc atggtgctgc aagcggctat
    10141 tcacgggcag ggagtggcgc tggcaaataa cgtgatggcg caatctgaaa ttgaagccgg
    10201 acgtcttgtt tgcccgttta atgatgttct ggtcagtaaa aacgcttttt atctggtttg
    10261 tcatgacagc caggcagaac tgggtaaaat agccgccttt cgccaatgga tcctggcgaa
    10321 agccgctgct gaacaagaaa aattccgctt tcgttatgaa caataattta cgtagggtac
    10381 gaccatgacc agccgtttta tgctgatttt cgccgccatt agcggcttca tttttgtggc
    10441 tctgggcgct tttggcgcgc atgtgttaag taaaaccatg ggggccgttg agatgggctg
    10501 gatccagacc ggcctcgaat accaggcgtt tcatacgctg gcgatcttag gtctggcggt
    10561 ggcaatgcag cgtcgcatca gtatctggtt ttactggagt agcgttttcc tcgcgttagg
    10621 cacggtgctg ttcagcggca gcctttattg cctggcactg tcccatctgc gtttgtgggc
    10681 gtttgtcact ccggttggcg gcgtgagctt ccttgcgggc tgggcgttaa tgttagttgg
    10741 tgctatccgt ttaaagcgca agggcgtaag tcatgaataa ggttgtattg ctgtgccgtc
    10801 cgggctttga aaaagagtgc gccgcagaaa ttaccgataa agccggccag cgggaaattt
    10861 tcggttttgc ccgcgtgaaa gagaatgcgg gttatgtcat ttatgaatgt tatcaacctg
    10921 atgatggcga taagttaatc cgtgagctgc cgttcagttc attaattttt gcccgccagt
    10981 ggtttgtggt gggggaactc ctgcagcatt tgccgccaga agatcgtatt acccccattg
    11041 tcggcatgtt acagggcgta gtagagaagg gcggtgaact gcgtgttgaa gttgccgata
    11101 ccaacgaaag caaagagttg ctgaaattct gccgtaaatt caccgtgccg ctacgcgctg
    11161 ccttgcgcga tgccggggtg ctggcgaact atgaaacgcc gaagcgtccg gttgtgcatg
    11221 tattcttcat tgcaccaggt tgctgctata ccggttactc atacagcaac aataattcgc
    11281 cgttctatat gggcattccg cgcctgaaat ttccggcaga tgcgccgagt cgttccacac
    11341 tcaaactgga agaggcattt catgtgttta ttcctgcgga tgagtgggat gaacgcctgg
    11401 cgaacgggat gtgggcggtg gatttgggcg cttgcccagg cggctggacc taccaactgg
    11461 tgaagcgcaa catgtgggtt tattccgtcg acaacggccc gatggcgcaa agtctgatgg
    11521 ataccgggca ggtgacgtgg ctgcgggaag acggtttcaa attccgtccg acacgcagca
    11581 atatctcctg gatggtatgc gatatggttg aaaaaccggc taaagttgcg gcattgatgg
    11641 cgcagtggct ggttaatggc tggtgccgtg aaaccatttt caatctcaaa ctgccgatga
    11701 aaaaacgcta cgaagaagta tcacacaatc tggcgtatat tcaggcacag cttgatgaac
    11761 atggcataaa tgctcagatt caggcgcggc agttgtatca cgatcgcgaa gaagtgacgg
    11821 tgcacgtccg ccgcatctgg gctgcggtgg gtggtcgtcg cgacgagcga taacaggcaa
    11881 ggccggggcg tcgtctccgg cctgctaccc tttccgtcag gctgttacca aagaagttgc
    11941 aacctggcgc tcatcagaaa taatgtgcgt tacctcatcc agatgcccga tgcgggcttc
    12001 ccctgaacga ttaaatttac ttttatcaat caataacaac gattgcgcgg cacgttttaa
    12061 tagcatcgat ttgtaatcag cgttgatggc attggagtcc cacagtgcgc cgctgctatc
    12121 gatcccttca caagaaaaaa taaacagatc gatttccagc gatttaagtt gggaaatcag
    12181 cgagggattg acgtaacagc catatttgcg ctcaagtgtg ccgccggaac tgatcagttg
    12241 aatgcgttcg cgtttaccga gttcatggca aatcggatgg ctattggtga agacctgaat
    12301 gttgatgtca ggcaactggc gtgccagata ccagcaagtt gaactggcgt ctaaggctat
    12361 caccatccct tcttcaatcc acgcgagcgc ctcgcgcgcg atatctgctt tatgcgcata
    12421 atggcttttc agccggatgt gaaagggatc gccactgtct tgattttgac ggtggatata
    12481 tttagcgcgt ccatgattgc gcagaatttt accctgcgtc tgtaattcat tgagatcgcg
    12541 acgaatggtt tctttactga cctttagctg ttcagagaga gcttccgtgg tcaggctggt
    12601 atggttcagc agcaggtcga ctatcgcttg ctggcgtgcc gctttcatat ttttcacccc
    12661 atcattcgat aaccgcccgc gtcatgcggg cgtaccggta tgagattacg gtgttacccc
    12721 tttttttaaa agaatattcc cgtacttcgc tcgttcgcct gtgataacga tcgcaaaggc
    12781 tttttgcgcc cgttcataaa acgcaaaacg attgatgcgg atgatgtcag gacacggggc
    12841 ttgtagtgaa agcgcattac ggtaacgtcg ttctacttca ggatcgagag tgtcaccttc
    12901 taccgctgcc atcatcacca gcggcggggc ataactgtcc agctcaaata acgggataat
    12961 cgcctggagc aagtcgctca ccaacaggcc atcagcgcgg atcacctgcg gtcccatcga
    13021 atgggcggga aagtgagcat cggaaaaaat aatttcatct ccatgtccca tctctgccag
    13081 cactttcaat agttcgggag aaattaacgg cgaaattgtt ttcagcattt cacacttcct
    13141 ctataaattc aggttcagtt tgcgggtaga aataacggta ctgataatga atctgtgcac
    13201 gggcttcttc cgggctgttg aattccccta cgccatacca accgaacagc gcagctcctg
    13261 cgacggtcgt ttcggcatcg tcgagaactt ttaccggaat atcaagcata ttggctttaa
    13321 tctgattcca caatgtgttg cgacttcctc caccgactaa caataattca gaggccttaa
    13381 agtgcccgat tttttccagc atctgtagat tgcgctgtaa ttgcgcagtt aacccttcca
    13441 gcgccgcgcg atagaaatgc ccccgcgtgg tattaagcgt cactccttgc cagccagcgt
    13501 tctgacacga caataaatca cactgcattt ttacgccatc cgcgccaggc gcgatcagac
    13561 gagcttcttc aatcaacatt tgccagggtg tttcagccgt ccagaacagt tttctcaccc
    13621 attccagcac gccggatgcc agccattgca tacctgggtt atacaaccct gcctggctat
    13681 ccagttcgca ggtggaaccg gcgtactgac ttaacagcga agtatcaacc tgggcgctac
    13741 ggaccattaa aatttcccat gtaccggaag agagcacggg ttcattttgg tcggcaccag
    13801 cgccaaaaag ggcgaactgg gtatcgtgac ctgcggaaat caccggtatg ccaacgggta
    13861 agccgagcat tgctgcggcg ctgttctgta gcgtaccaat ctgttcaccc gcttccacca
    13921 gacgagggaa gagtcggcgt ggaataccgg tggcttgtaa aatttgcgga ctgaaatcgc
    13981 gttgctggat atccagcatc tggctggttc cggccatcgt gatatcagta gtgaattcgc
    14041 cggttaaacg gtggttaatc agcgacgaaa taaagagcca ggcgtgcgcg cgttccagca
    14101 gttgtggatg attttctttc aaccacacca acttatataa cgtattgaaa ctaaaggctc
    14161 cgacgccaga aatagcctgc aaccgctgtg cggagatcaa ctgttcaata ttgtccataa
    14221 ccgctgctgt tcgcggacat ttccagctaa tgatcggata gagcagattg ccttgcttat
    14281 ctaccagagc gccatccaca ccaaaggtgg tgacggcgat accgcggatg tggcattcag
    14341 tcagttcact attgatttgc cgacagcaat cagcaaagcg ttgcaaaatg gcgtctaaag
    14401 accactggtg ccaggtgttg ttttccatcg cgatatcgct ggcattaggc gttgaggcgc
    14461 gggcaacaat ttttccctgc cgattaaccg cgatggccct gacattggtc gcgccacagt
    14521 cgagtaccag gataacttct tgtttcataa tcgctcctgc aatatagccg gataacattg
    14581 cttatccggc taaccactct tgcttgttca ggcccggtag ccggagcgac cgggcatcac
    14641 atcagggagt aatgtattaa cgcttgtaca acgggccgta gttctggcaa gcgcggtaat
    14701 cctggccttc aatatccatg ccgtgcgcag cccaggcaga aggacgatac actttggtct
    14761 cttcaacgtt gtgcatacat accgggatac gcagcatgga ggcgagagtg ataaagtctg
    14821 cgccaacgtg gccgatggtc agaaccccat ggttagcgcc ccagttcgcc attaccgagt
    14881 acacatccgt aaacggccct ttaccggtga ggcgcggtgc aaaccaggtg gttggccagg
    14941 ttgagttggt gcgtttgttg aggatgtcat gcacatcctt cggcaattcc acgctccagc
    15001 cttccgcgat ttgcagtacc ggtcccaggc ctttgatgat gttgacacgg gtcatggtga
    15061 acgggacgcc gccttcggta aggaagcggg aagagtaacc gccgccacgg aagtattcgt
    15121 ggatcgccgg gcaccattcg gtagcggcga ggcaagcgtc agcctcttgc tgagagattt
    15181 cccagtgcgg cttcatcgtc gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca
    15241 gcgcagcaga accggagttg atcaaatgga tgatgccgtg ttctgccagt ccatcgagtt
    15301 tatgccccgt tacacgctca attgcttctg gtgaccagta ggtacgcaca tcggcaaata
    15361 cctgagcggt gccggtgagc tggtgaccca ttagcattgc cacgccgtta agactgtcgt
    15421 tttcggtcgc cacgacaaag ggttcgcgca cgccattcca gtcaaatgaa ctgttaagga
    15481 tcgcttcggc ggtgtcacca ttgggatatt gatcggtcca gtgacgttgc ccctggaagc
    15541 ccgcagcgat ggcgttgtag ccaagtgatt cttccacgcg accaatatcg gccagtttgc
    15601 tgttgccttg catcatgtcg cggatacaca tcgccatcag taaactttcg cgcagaactg
    15661 cgcggcgttg ctcggcattg cgttgatact gtttgttatt ttcatcttcg ccatagcgga
    15721 agtttttatc agcccaggcc agtgccattt ccagttcggc ttcgtcgtaa atcttctgat
    15781 cgatacggcg acgcagttcg gtcatatcca ccgcctggac tttcattccc agccaggatt
    15841 caaagaagtt gtgatcaaca atggaaccgg cgatacccat cgaaacgccg cccagcgaca
    15901 gatagctttt acctttcatg ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt
    15961 tttcttcaac atcggcagga atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa
    16021 tggagaatgc cgggatgcct ttctggctgt gagctgccag agccgctgcc aggtaaacag
    16081 cgccgggacg ttcagtgccg ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt
    16141 cgatggtttc actgccatag caccagcaag gcgttaccgt aatggtgagg cctacattct
    16201 gactgctgaa tttttcttcg caagcagcgg cttcagccat acccgcgata caggtatcgg
    16261 aaatgacaca ctcgacggca gctccgcagg catggcgcag tttctcggtc agcagtgcgg
    16321 ccgtagcttt cgccatattc attgtttgtt cttcaagcga ctcacgaaca cccatgcgac
    16381 gaccgtcaat aaccgggcgg ataccaattt tcggtaagct gatttttttc attacagatt
    16441 cctcacttta ttcggaaaat atgttcagtt agttgccgtt tgagagcgga aacgggcaaa
    16501 gataaagatg accgcgaagc agagtgcggg gatcagttca gcagtgggga tgttgcccgc
    16561 cgcgtcactg acaaaaccca tgaccggagt gacaataccg ccgccaatga tggtcataac
    16621 gatgaaggac gaaccgtatt tggtgtcctg gccgagattc ttaatgccca gcgagaagat
    16681 tgttgggtac tgaatcgaca taaaggcgct gcataaagtc agggctatta agcccacatg
    16741 accgccagcg aaggctgaga tcaggcacag tgccatagcg attaatgcgt aggcggccag
    16801 gactttgtgt ggtgcgaagc gactgatgag ccaggtaccg gtgaaacgac caataaagaa
    16861 gcacaccatg gttccggtta aatagttagc ggcaaagcct gcagtcatac ctggaatttc
    16921 ttctacagcg tagcgaatca aatagctcca gcaggccgtt tgtgcgccaa cgtagcagaa
    16981 ttgcgctaat actgcccagc gccagtggcg aatacgcgcc aggcgagaaa gcgatgcgga
    17041 gaacgatcct tgtttggcgt cactgtgatt atcactctgc aatgccggga atttcgtcag
    17101 catgatcagc agggcgacca gtaacacgat agccacgatg atcatataag gtgtctgtac
    17161 cgataatacc aggctgtgtt tatacgcact caattgctct ggagacattt tatcgagaac
    17221 gtcttgcgat tgatgtggca cgttagacaa aataagactt tgcccaaaga caaccgcgat
    17281 aattgcgcca aacgagttaa atgtttgcgc aagatttaag cggaagtgac cactgctttc
    17341 cggccctaat accgtaacaa aagggtttgc ggcagtttcc agacaaccta atccggctgc
    17401 aataataaat aggccaacta aaaataaggt gtagttcatt atttctgcag cgggccagaa
    17461 taatgcagcg cccaaggcat ataaaaataa tccggtaata atccctgctt tataactgag
    17521 ttttttcatc aatatcccag cagggattgg gataatgaaa taaccaaagt aaaaggccga
    17581 ttggatcagg ccagcctgga aatttgtcag cgtaaaagcc tgctggaatt gaggtaataa
    17641 aatgtcgtta aggttattgg ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa
    17701 tggaataatg taacttctgc tttgccctgc atctttatct accgcacggt aactctgcgt
    17761 ttgtattgat gtgtttccca tagcatcctc ttaggttcag aagcttaagc acgtaatcgg
    17821 ttatgttttc aaaagtgcaa aaccagcgag ttcgggcttc atatcagtga atatttgcct
    17881 gtctgaaatg aactcatgga ttcattttca aataaaaaac gctctgtaat atgacggcgg
    17941 tcacacttat tagctggaag gtgactttat gtgactacca tcactttaat aatcgaaatt
    18001 tcatgcccgt ttcagaaaat aaatcataaa aaacacaaac cagatgaccg tattaattgt
    18061 ttatgccgaa aacgggcatt cctggcttga gtgaatagaa aaatgaccgt tttgataaat
    18121 atttttaaga gcggtcattt ttaatggggt tataaaaaga gaacttgcag ttatttactg
    18181 tgattagttg aaccaggtca caaaatataa gcttgtgaat ttctttctgg ttaatcagga
    18241 agaagatggt gggtaattaa acggctaatt caatagtgtg aaaggaacaa cattattgcc
    18301 ctgttttgaa tcagagagag gtagctaatg gaacgaaata aacttgctcg tcagattatt
    18361 gacacttgcc tggaaatgac ccgcctggga ctgaaccagg ggacagcggg gaacgtcagt
    18421 gtacgttatc aggatgggat gctgattacg cctacaggca ttccatatga aaaactgacg
    18481 gagtcgcata ttgtctttat tgatggcaac ggtaaacatg aggaaggaaa gctcccctca
    18541 agcgaatggc gtttccatat ggcggcctat caaagcagac cggatgccaa cgcggttgtt
    18601 cacaatcatg ccgttcattg cacggcagtt tccattctta accgatcgat ccccgctatt
    18661 cactacatga ttgcggcggc tggcggtaat tctattcctt gcgcgcctta tgcgaccttt
    18721 ggaacacgtg aactttctga acatgttgcg caggctctca aaaatcgtaa ggcaactttg
    18781 ttacaacatc atgggcttat cgcttgtgag gtgaatctgg aaaaagcgtt atggctggcg
    18841 catgaagttg aagtgctggc gcaactttac ctgacgaccc tggcgattac ggacccggtg
    18901 ccagtgctga gcgatgaaga gattgccgta gtgctggaga aattcaaaac ctatgggtta
    18961 cgaattgaag agtaatttcg taaagcaaca aggagaagga tgatggctaa cagaatgatt
    19021 ctgaacgaaa cggcatggtt tggtcggggt gctgttgggg ctttaaccga tgaggtgaaa
    19081 cgccgtggtt atcagaaggc gctgatcgtc accgataaaa cgctggtgca atgcggcgtg
    19141 gtggcgaaag tgaccgataa gatggatgct gcagggctgg catgggcgat ttacgacggc
    19201 gtagtgccca acccaacaat tactgtcgtc aaagaagggc tcggtgtatt ccagaatagc
    19261 ggcgcggatt acctgatcgc tattggtggt ggttctccac aggatacttg taaagcgatt
    19321 ggcattatca gcaacaaccc ggagtttgcc gatgtgcgta gcctggaagg actttccccg
    19381 accaataaac ccagtgtacc gattctggca atccccacca cagcaggcac tgcggcagaa
    19441 gtgaccatta actacgtgat cactgacgaa gaaaaacggc gcaagtttgt ttgcgttgat
    19501 ccgcatgata tcccgcaggt ggcgtttatt gacgctgaca tgatggatgg tatgcctcca
    19561 gcgctgaaag ctgcgacggg tgtcgatgcg ctcactcatg ctattgaggg gtatattacc
    19621 cgtggcgcgt gggcgctaac cgatgcactg cacattaaag cgattgaaat cattgctggg
    19681 gcgctgcgag gatcggttgc tggtgataag gatgccggag aagaaatggc gctcgggcag
    19741 tatgttgcgg gtatgggctt ctcgaatgtt gggttagggt tggtgcatgg tatggcgcat
    19801 ccactgggcg cgttttataa cactccacac ggtgttgcaa acgccatcct gctaccgcat
    19861 gtcatgcgct ataacgctga ctttaccggt gagaagtacc gcgatatcgc gcgcgttatg
    19921 ggcgtgaaag tggaaggtat gagcctggaa gaggcgcgta atgccgctgt tgaagcggtg
    19981 tttgctctca accgtgatgt cggtattccg ccacatttgc gtgatgttgg ggtacgcaag
    20041 gaagacattc cggcactggc gcaggcggca ctggatgatg tttgtaccgg tggcaacccg
    20101 cgtgaagcaa cgcttgagga tattgtagag ctttaccata ccgcctggta aatgcgctga
    20161 tgtgataatg ccggatacga cgtttgcggc gtatccggct cgccgttacc gtaccaaccg
    20221 caattgctgt aaattgccgt cgatatgcaa atcggtttgt aagcgggcaa tatcgcggca
    20281 cagaaacgcc atctctttat gggtttctaa ttttttgcgc cacttttcgg caaccgcatc
    20341 cagattctca tatatccctt ccagactctg aaactcgacc agcagctgcg tggcgctttt
    20401 tggtccgatt cccgcaacac ccggtacctt tgaactgctg atccccgcaa gtccccagta
    20461 atcgggcaac tgctgcggtt gaatgccaaa ttctttatcg ataaatggcg catccagcca
    20521 acgtttctgg aagtaatcac gaatacgtaa tgtcggtgaa agtaactgac agtagccttt
    20581 atctgtcgaa acaatcgttg cctgatgccc ggcctgtgtc actttgaccg ccagcgtggc
    20641 ggctaagtca tcggcttcgt tgccgctggt tgaccagcac gggacgccgc gttgctcaaa
    20701 ggcggcgcgt aatgcaggca tctcgtcgtg caactcttcc ggcattggcg gtcgacccgc
    20761 tttgtaatct ggtaaacgct gatgacgcca gccgctactg cggttttcat catcaaaaac
    20821 ggcgaccgcg tgggttggct ggctgtgcat aatgagctga tcgagcgcat gctggcaggt
    20881 ttcgacacag ggcgatccct gaacggcatg aatgcgacga ataagattca gtgcatcgac
    20941 aataagcaaa tgaacagcca cgataacccc cttaaagatc aggcgataag ggtaacattc
    21001 gatgccggaa caggctacgg ctggatgaga aatcgggaag aggcctcgca aaacgaggcc
    21061 tctggagagc gattaatcgc aggcaacgat cttcattgcc aggccgccgc gagaggtttc
    21121 gcggtacttg gcgttcatat ctttacctgt ttcgtacatg gtttcgataa ctttatcgag
    21181 gcagacgcgc ggctcgctgg tacggcgtag cgccatacgt gcggcgttca ccgcttttac
    21241 tgccgcaatg gcgttacgct cgatgcatgg cacctggacc tgtccggcga ccgggtcaca
    21301 cgtcagaccg aggttgtgct ccatggcgat ttccgccgcg atgcacacct gcgccgggct
    21361 tgcacctaac agttctgcca gaccagccgc cgccattgag cacgccacac caacttcacc
    21421 ctggcagccg acttcagcac cagaaattga cgcgttcatc ttataaagag aaccaatggc
    21481 actggctacc agcaggtaac gagccagtga gttagcgttc acttcgcgga taaacttgtc
    21541 gtagtacgcc agcactgccg ggataatccc gcacgcaccg ttagtcggcg cagtcaccac
    21601 gcgtccgcca gcagcgttct cttcgttcac tgccaatgca aacatgttga tccagtcaac
    21661 aaccgccatc gggtcagtgg tggttttatc ctggctgacc agcatccggc gtagtgccgc
    21721 agcacggcgt ggaacgcgca gtttgccagg caacacgcct tcggtggaaa taccgcgctc
    21781 aataccgcca cgcatgactt cccatacgtt cgccaggtgc tgttccagct cttctttgct
    21841 gtgcagcgcc agctcatttt tcatcatcag gccagagagt gacagcccgg tttcctgaca
    21901 atgtttttgc agatcggctg ctgaactgta cggataagga acttcaaccg gtgcgctatt
    21961 ctgctggcca aaatgctctt catcaacgat aaagccaccg ccaatagagt agtaagtctg
    22021 gctgtaaacg actttatcgc ccgccagcgc ggtaatgcgc ataccgtttt catgcagaga
    22081 aaggttgtct gcatggaagt tcatgcactg atcaaccggg aactccactt catgctgacc
    22141 gtttgccagc atcaggcgac catgagtatt cacatcctga ataaaaccag ggatggaatc
    22201 gatatccacg gtatccggca ggttacccgc caggcccata ataatggcga tatcagtgtg
    22261 gtggccttta ccagtcagag agagcgagcc gtacacgtca accaccacgc gggtcacgtc
    22321 tttaagcagg ttacgggcaa tcagatcgtc ggtaaattgt ttacccgctt tcattggtcc
    22381 aacggtatga gaactggaag ggccaatgcc gattttgaaa atatcgaata cgctaatcat
    22441 aggaaataca tcgcgttaaa acggaggaag cgccgcccga aagcggcgcg aaaggactta
    22501 gctgaacaga gagtagaaga ttgcggagat tgcaatcaga cccatcacga caacgaatac
    22561 gttgctgatg tgaccgctgt acttacgcat tgccggtact ttctgaattg cgtacatcgg
    22621 catcaggaac aggatcatcg cgatgattgg gccgcccagg gtttcaatca tacccaggat
    22681 gctcgggttc agggtggcaa caatccaggt cgttaccagc atgaacagcg cagtgatacg
    22741 gttcagcttg ttgatttcga tagacttacc tttaccacgc agagatttaa tcaccatacc
    22801 gttgaagcct tcacgtgcgc ccaggtagtg gccgaggaag gatttggtga tagcgataat
    22861 cgcgataatc ggagccatcc acgcgataac cggtgcgtta aagtggttag ctaggtaaga
    22921 cagaatcgag atgttctgct ctttagccgc agccaggtct gccggagtca ggctcagtac
    22981 gcagctgaag acgaagaaca ttacggtcag caccatcatg atgtgtgcga atgccaggat
    23041 cttcgagcat ttctgttctg ccatatcgcc gtactcttca cgcttcgcaa cggcgaaaga
    23101 agagatgatc ggagagtggt tgaacgagaa caccattacc ggaattgcca gccacagggt
    23161 catccacaga ccgtttccgg ttgcagatgc agtgtccaga gacagcgttt ccagtgctgc
    23221 gccgttccac tgcgggatca ggtacagagc cagcagcatc agtacgccaa caaacgggaa
    23281 taccagaata ctcatcgctt taacgatcat ctgctcacca aagcgaacga tggtcatcat
    23341 acccacgatc aggatcagcg acagaatcgc acgcggcggt ggcgtcatac ccagctggtg
    23401 agacatgaag ctttcaactg tattggtgat tgccacgcta taaaccagca ggatcgggta
    23461 gatagcgaag aagtagagca gggtaatcag tttacctgcg ccaataccaa agtgttcttc
    23521 tacaacctcg gtgatgtctt cgcccgggtt tttaccagac agtacgaagc gagtcaggcc
    23581 gcggtgagca aaaaacgtca tcgggaacgc aaggatagcc atgatgatca gcgggatcat
    23641 accgccaaca ccggcgttga ttggcaggaa cagcacgccc gcgccgattg ccgtgccgta
    23701 aaggcccagc atccacatgg tgtctgtctt gcgccaggca ctacgagagt ctttcgacgc
    23761 aatcgtgctg gtttgagtcg tttccatcta tttctcctgg aggaatgcaa ataattcagc
    23821 ttttagccag ttcagcccgg gaaggtgcct gaccgtaaaa atgaaatctg tctatcggcg
    23881 atggcttaat catttaccgc cgtaataatt tgagggcgga aagatacaca caagttatgt
    23941 atctatcagt gatcttgatc ccaaatacaa taatctactt ggaagataga ttatcccaac
    24001 gctttactct gtaaatcaaa agtaaaagag aatttaaggg caatatttgc ccattaaacg
    24061 acatctgact tcttccgggg cgcgcaatat atgtaggttg aactgaaaaa atcaatactt
    24121 ttagttaatc atggcgataa tttctttttc atagcgataa aatacggttt atgaaaatag
    24181 ggaaacgttt gcgttgcaat ataaattgta tgacatatta actgtgatga tatttgtcgc
    24241 gtgagggtgg taagaaacat tcatataaca aatgaaatga gttgatgagt aagtgaccaa
    24301 aaacgagaaa acccggcaac aaacgtcacc gggggagggg agattacgtg cagatttcgt
    24361 agcaagggat gtaggctgag cctggcaact tcatacgatg ctgggcaaca aaaccctgta
    24421 gcaggtcgtc catacgacgc ataatctctt tatcgccgtt gattttgtaa ggaccaaact
    24481 cttcaatggc gcgaataccg acttctttta cgttacccgc cacaataccg gagaacgcac
    24541 ggcgcaggtc ggcagccagc acttcaacag gttgatccgg gtaaagcttc agattagcca
    24601 tattctcgtg agacggctca aacggcattt gcaaatctgg tgcaatgcgc attgaccagt
    24661 taaagctgta agcgtcacca gtgtcgcggc gattttcttt caccagcggc atcgattttt
    24721 tcatctgacg agcgacttca gcggcgtcat caatgatgat gcggtaatgg cggcgcgcgt
    24781 tttcgcccag cgtatgtacg acaaactcgt ccagtacgcg gaagtagtcg gcgctctctt
    24841 tcgggccggt gaggatcaat ggtaaaacct gatctttgtt ggccgggttc attaaaattc
    24901 ccagcaaata cagcaactct tctgccgtac ccacaccgcc agggaagata atgataccgt
    24961 gagcgatacg gacaaacgct tccagacgtt tttcgatatc tggcatgatg atcaattcgt
    25021 tgaccagcgg gttaggcggt tcagcggcga taatcgacgg ctcggtcata ccaataaaac
    25081 gactgtcttt gtaacgctgc tgcgcgtgtc cgaccgcagc acctttcatc ggcgcttcca
    25141 tcgctcccgg accacagccg gtgcagatat tcagctcacg caggcccagc tggtttccga
    25201 cgcgacgggc atacaaatac tcgttttcgt taattgagtg accgccccag cagaccacca
    25261 tatttggcgc ttcacccaca tgcagcgcgc gagcgttacg caagatggaa aagaccaggt
    25321 tagtgatgtg gacggagttg tcgagattca ggttgggaaa acgaacggta ttatggattt
    25381 gcccgtaaac gaagagaatg tctcgcagaa ccgcgaacaa gttggcctgc aaagcgcgaa
    25441 taattcgccc atcgacaaaa gcctcttccg ggggattaat cagttccagc tttacgccgc
    25501 gttcacggcg caggacgtta atatcgaaat tttcaaaacg agacagcaat tctttgctgt
    25561 tatcggtcaa actaccggag ttcagtacgg caagtgaaca gttgcgaaac agttgataga
    25621 ggtcgctgct ggcggtgcgt ttaagcatat ccacttccag ctgcgacaac atatccatgg
    25681 agccaagcgg gctaatatgt gtaatcaaga aaaactcctt atgggacgaa tatcgtcatt
    25741 ccctttgatt acaatagccc tgcctgatga cctttcacaa ccttctgcgt gaatccagca
    25801 cgcaaaattg agaaaaaatt tattgccgaa ccagtcttgt ggtcgatggg acaaaatcgc
    25861 tattactgcg ccacgggtta atgtccagac cgccacgacg ggtataacgt gcgtaaacgc
    25921 ttaatttctc tggctggcag aagcgtaaca ggtcattaaa gatgcgttcc acgcactgtt
    25981 cgtggaactc gttgtgatga cggaatgaga ccaggtaacg cagcagtttt tctctgtcaa
    26041 tttggcgtcc acgatactga atttggatcg aaccccaatc tggttgatgg gtgatcaggc
    26101 agtttgattt cagcaggtgg ctgacaagcg tctcttccac tactttttca ccgctggtgg
    26161 cattctccag atagtcagta gtgaattcat agttatcgat agtgatatcc tggtcatcaa
    26221 tgcaggtgcc attgaaatga cctatcggct ggccttccag ttcatcaaga cgatataacg
    26281 ccacgctaac cttaccctgg gcgcaagtgc ttaagtcgcg ctccagcgtc tggcgcacct
    26341 catcccagtt attaaaacgc gtctggttaa aactgttgag atagagctta aaactcttcg
    26401 actcaatcag atttgcgctg gtgtaatcaa gttcaacgtg accgacggca acctgcggca
    26461 aacctttcgc attcagccag gaaagttcat acagcgtcca gatatccgta ccgtgaaaag
    26521 gcaggttatc cgctttcaga cccagcgggt cgcgattcag gctgcgtgga acgccttgca
    26581 gtaggttggc gtcataggta tcccggtaat cggttgattt tccaagagtc aggcccgcaa
    26641 gtgcctgatg gtttgcataa gaagacatgt ttcaccgtca taaaccagat aaactaaacg
    26701 acaagtgtat ctggtttaag tgaagagaga aattagtgga cgatttgacc gcacaagccc
    26761 tgaaagattt tactgcacgc tactgcgatg cgtggcatga agagcataaa agctggccgt
    26821 taagtgagga actgtacggt gttccttcac cgtgcattat ttcaactacc gaagatgccg
    26881 tatactggca accccagccg tttaccgggg aacaaaatgt aaacgcggtt gaacgcgctt
    26941 ttgatattgt gatacaaccc acaattcata ccttttatac gactcagttt gccggggata
    27001 tgcacgcgca gtttggcgat atcaaactga cattgctgca aacctggagt gaagacgact
    27061 tccgtcgggt gcaggaaaat ctgattggtc atctggtgac tcagaaaagg ctaaaactgc
    27121 cgcccacgct atttatcgcg acgctggaag aagagttaga ggtcatctcg gtgtgtaatc
    27181 tctccggtga agtgtgcaaa gagacgctgg gaacacgtaa acggacgcat ttagcctcaa
    27241 atcttgcgga attccttaat caacttaagc ctcttctgta atccaaatac cccatctggt
    27301 tgtgagagat ctcttacaga ctctgtagga gatcgccagg aaataagcga atacttaaaa
    27361 agataagaat cgttattttc atttaaaatc aaaatgttga ttgttaattc ttaactttca
    27421 tatgaaattt tccttaaggc attgtctgta agcgccttgt aagacttcgc gaaaaagacg
    27481 attctatctt cgtcgacagg gagtcagaca acgaagtgaa catcaggatg atgacacttc
    27541 tgcaggacac accaggatgg tgtttcaggg aaaggcttct ggatgaagcg aagaggatga
    27601 cgcaggacgc gttaaaggac acctccagga tggagaatga gaaccggtca ggatgattcg
    27661 gtgggtcagg aaggccaggg acacttcagg atgaagtatc acatcggggt ggtgtgagca
    27721 ggaagcaata gttcaggatg aacgattggc cgcaaggcca gaggaaaagt tgtcaaggat
    27781 gagcagggag caacaaaagt agctggaatg ctgcgaaacg aaccgggagc gctgtgaata
    27841 cagtgctccc tttttttatt cctgctatcc ttcgcggcag tttttcttat tgaggttgct
    27901 ttatgaccac tcatgaccgc gttcgtctcc agttgcaggc gcttgaagcg ttactgcgtg
    27961 aacatcagca ctggcgaaac gatgaacccc agccgcatca atttaatagt acccaaccgt
    28021 tctttatgga caccatggaa ccactggagt ggttgcagtg ggtgctgatc ccgcgaatgc
    28081 acgatttgtt agataacaaa cagccgttac cgggcgcttt tgccgttgca ccctattatg
    28141 aaatggcgct ggcgacggac catccgcaac gcgcgcttat tctggctgag ctggaaaaac
    28201 tggatgctct ttttgcggat gatgcgagct aatgctggaa atactctatc aggatgaatg
    28261 gctggttgcg gtaaataaac cctccggctg gctggttcac cgcagctggc tggatcgcga
    28321 cgagaaagta gtggtcatgc aaaccgtgcg tgatcagatt ggccagcacg tttttaccgc
    28381 tcatcgtctg gaccgaccca cttctggtgt gttgttgatg ggattatcca gcgaggccgg
    28441 acggctgctg gcacaacagt ttgaacagca ccaaatccag aaacgttacc atgcgattgt
    28501 gcgcggctgg ttgatggaag aagcagttct ggattatcca ctggtggaag aactggacaa
    28561 aatcgctgat aaatttgccc gcgaagataa aggctcgcag ccagcagtga cgcattatcg
    28621 cggtctggcg accgtagaaa tgccggtagc gaccggacgt tacccgacca cgcgctacgg
    28681 cctggtggaa ctggagccga aaaccggacg caaacatcag cttcgccgac atcttgccca
    28741 tttgcgtcat ccgattattg gcgatagcaa acatggcgat ttacgccaga atcgcagcgg
    28801 tgctgagcat tttggcctcc agcggttaat gctacatgcc agtcagttgt cattgacgca
    28861 tccttttact ggcgagccgc tgactattca cgcgggcctg gacgacacct ggatgcaggc
    28921 gttatcacaa tttggctggc gcggtctgct tcctgaaaat gaaagggttg agttcagtgc
    28981 gccatcgggt caggatggag agataagttc ctaatccagg gagaaaacgg tatggcggaa
    29041 attggtattt ttgtcggcac catgtacgga aattcgctgt tagtggcgga agaagcagaa
    29101 gcaattctga ccgcacaggg ccacaaagca acggtatttg aagatcctga attaagcgac
    29161 tggctgccct atcaggataa gtatgttctg gtggttacgt ccacgaccgg gcagggcgac
    29221 cttcctgata gcattgtgcc gctctttcag ggaatcaaag atagtctggg cttccagccg
    29281 aatctgcgtt atggcgtgat tgcgctcggc gacagcagtt atgtaaattt ctgcaatggc
    29341 ggcaaacagt tcgatgcctt gttgcaggaa cagagcgctc agcgggttgg cgaaatactg
    29401 ttgattgacg ccagtgagaa cccggaaccg gaaacggaat caaatccgtg ggtcgaacag
    29461 tggggcacgc tgttgtcctg atatgttcag cgagcggtaa atgtcgtttt agcggtgctg
    29521 aatcgaatct tttcaggcaa atgccagtaa aaactgcttc atagcgcgga tttttactgg
    29581 cgtttgcctg gagtcaagcg atccatttca tactcttctt tatttcttcg tttttaccct
    29641 tcctttcttg ttcttgtttt catttccgtg aagtggattc caccgtccag ggctaatgcc
    29701 aaaatcgggc ctcattgaac gcattaatgt tgtgttgttg cacggtgagc cgctatggcg
    29761 cgctttttat actgctattg ccagatataa acacgcgccg tattcggcga acgacctata
    29821 aaaacggcaa aaaacaccct acgtcacctc tgatttcctg gcgatgtcgc agtccagagt
    29881 gagcgtggct aacgcgaatt ttcaggagtg caacaatgag ttctttaagt caggctgcga
    29941 gcagtgtgga aaaacgcacg aatgctcgtt actggatagt ggtgatgttg tttatcgtca
    30001 catccttcaa ctacggcgac cgcgctacgc tttctattgc tggttcggaa atggccaaag
    30061 atatcggcct tgatcccgtg ggaatgggct atgtgttctc tgccttctca tgggcttatg
    30121 ttatcgggca gatccctggt ggttggttgc tggatcgttt tggttcaaaa cgcgtctact
    30181 tctggtcgat ctttatctgg tcgatgttta ccttgctgca aggcttcgtc gatatcttta
    30241 gtggattcgg cattatcgtt gccctgttta cgctgcgctt cctggtcggg cttgctgaag
    30301 cgccatcttt ccccggcaac agtcgcattg ttgcggcctg gtttccggcg caggaaaggg
    30361 gaacggcggt gtcgattttt aactccgctc aatacttcgc aacggtgatc ttcgcgccga
    30421 ttatgggctg gctgacgcat gaagtgggct ggtcacacgt cttcttcttt atgggcggtc
    30481 tggggattgt catcagcttt atctggttga aagtcatcca cgagccaaat caacatccgg
    30541 gggtaaatca gaaagagctg gagtacatcg ccgcgggtgg cgcgctgatc aatatggatc
    30601 agcaaaacac caaagttaaa gtgccgttca gcgtgaagtg ggggcagatc aaacaactgc
    30661 tcgggtcacg gatgatgatc ggcgtttata tcggtcagta ctgtatcaac gccctgactt
    30721 actttttcat tacctggttt ccggtttatc tggtgcaggc gcgtgggatg tcgattctga
    30781 aagcgggctt tgtggcttcc gttccggcgg tttgcggttt tatcggcggt gtgctgggtg
    30841 ggattatttc cgactggctg atgcgccgca cgggatcgct gaacattgcg cgtaaaacac
    30901 cgatcgtaat gggcatgttg ctgtcgatgg tgatggtgtt ctgcaactac gtcaacgttg
    30961 agtggatgat catcggcttt atggcgctgg ccttcttcgg taagggcatc ggggcgctgg
    31021 gttgggcagt aatggcagat accgcgccaa aagagatcag cggtctttcc ggtggcctgt
    31081 tcaacatgtt cggtaacatt tctggcatcg tcacgccaat cgcaattggt tatatcgttg
    31141 gcacgactgg ctcctttaat ggggcgctga tttatgttgg tgttcatgcc ttaatcgcgg
    31201 tactgagcta cctggtgctg gtgggcgata tcaagcgtat cgagctgaaa cctgtcgcgg
    31261 ggcaataaga tgacgacaca atccagtcct gttattactg atatgaaagt cattccggtc
    31321 gccgggcacg atagcatgtt gcttaatatt ggtggggcac ataacgcata tttcacccgc
    31381 aatattgtgg tactcaccga taacgccggg cataccggca ttggtgaagc gccgggcgga
    31441 gaggtgattt atcaaacact ggtcgatgct attccgatgg ttctgggcca ggaagttgcg
    31501 cgcctgaata aagtggttca gcaggtgcat aaaggtaatc aggcagccga ttttgatacc
    31561 ttcggcaaag gtgcctggac ttttgaattg cgcgttaatg ccgtggcggc gctggaagcc
    31621 gccttgcttg acctgctagg taaggcgctg aatgttccgg tctgcgaact gttggggcca
    31681 ggcaagcaac gcgagactat taccgtcctc ggttatctgt tttatatcgg tgatcggacc
    31741 aaaaccgatc ttccttatct ggaaaatacg ccgggcaacc atgagtggta tcagttgcgc
    31801 catcagaaag cgatgaacag cgaagccgtt gtgcgtctgg cggaagcctc acaggatcgc
    31861 tatggcttta aagatttcaa acttaagggc ggggtgttac ctggcgagca agaaatcgac
    31921 actgttcggg cattgaagaa acgcttccct gatgcgcgga ttaccgttga tcccaacggt
    31981 gcctggctac ttgatgaagc catttctcta tgcaaagggc tgaatgatgt tcttacctat
    32041 gccgaagatc catgcggcgc agaacagggt ttctccggtc gtgaagtgat ggcggagttt
    32101 cggcgggcga ccggcttgcc cgtcgcgact aacatgatcg ccaccaactg gcgcgaaatg
    32161 ggtcatgcgg tgatgctcaa tgcggtagat attccacttg ccgatccgca cttctggacg
    32221 ctttccggtg cagtccgtgt ggcgcagctt tgcgacgact gggggctgac ctggggctgc
    32281 cattctaata accatttcga tatctctctg gcgatgttta cccatgtggg cgcggcggca
    32341 ccgggtaatc ctaccgctat cgatacccac tggatttggc aggagggcga ttgtcgcctg
    32401 accaaaaatc cgctggagat taaaaacgga aaaattgccg ttcctgatgc gcccggtctg
    32461 ggcgtggaac tggactggga acaggtacaa aaggcacatg aggcctataa acgtctgcct
    32521 ggcggtgcgc gtaacgacgc aggtccgatg cagtacctga tccccggctg gacctttgac
    32581 cgtaaacgtc ccgttttcgg ccgtcattga ttctgaaaaa ggacataaat atgagttctc
    32641 aatttacgac gcctgttgtt actgaaatgc aggtcatccc ggtggcgggt catgacagta
    32701 tgctgatgaa tctgagtggt gcacacgcac cgttctttac gcgtaatatt gtgattatca
    32761 aagataattc tggtcacact ggcgtagggg aaattcccgg cggcgagaaa atccgtaaaa
    32821 cgctggaaga tgcgattccg ctggtggtag gtaaaacgct gggtaaatac aaaaacgttc
    32881 tgacgctggt gcgtaatact tttgccgatc gtgatgctgg tgggcgcggt ttgcagacat
    32941 ttgatttgcg taccactatt catgtagtta ccgggataga agcggcaatg ctggatctgc
    33001 tggggcagca tctgggggta aacgtggcat cgctgctggg cgatggtcaa cagcgtagcg
    33061 aagtcgaaat gctcggttat ctgttcttcg tcggtgatcg caaagccaca ccgctgccgt
    33121 atcaaagcca gccggatgac tcatgcgact ggtatcgcct gcgtcatgaa gaagcgatga
    33181 cgccggatgc ggtggtgcgc ctggcggaag cggcgtatga aaaatatggc ttcaacgatt
    33241 tcaaactgaa aggcggtgta ctggccgggg aagaagaggc cgagtctatt gtggcactgg
    33301 cgcaacgctt cccgcaggcg cgtattacgc tcgatcctaa cggtgcctgg tcgctgaacg
    33361 aagcgattaa aattggtaaa tacctgaaag ggtcgctggc ttatgcagaa gatccgtgtg
    33421 gtgcggagca aggtttctcc gggcgtgaag tgatggctga gttccgtcgc gcaactggcc
    33481 tgccgaccgc aaccaatatg atcgccaccg actggcggca gatgggccat acgctctccc
    33541 tgcaatccgt tgatatcccg ctggcggatc cgcatttctg gacgatgcaa ggttcggtac
    33601 gtgtggcgca aatgtgccat gaatttggcc tgacctgggg ttcacactca aacaaccact
    33661 tcgatatttc cctggcgatg tttacccatg ttgccgccgc tgcaccgggt aaaattactg
    33721 ctattgatac gcactggatc tggcaggaag gtaatcagcg cctgaccaaa gaaccgtttg
    33781 agatcaaagg cggtctggtt caggtgccgg aaaaaccggg gctgggtgta gaaatcgata
    33841 tggatcaagt gatgaaagcc catgagctgt atcagaaaca cgggcttggc gcgcgtgacg
    33901 atgcgatggg aatgcagtat ctgattcctg gctggacgtt cgataacaag cgcccgtgca
    33961 tggtgcgtta agacagaaaa ccaaaaccgg ctctatctgg agccggcttg agactgatgc
    34021 taaacgcaaa atagcctgtt gtgctatgta tcaggtctac atcatttttt aattcattga
    34081 atttaattgg ttttgtaggc cggataaggc gttaacgccg catccggcat aaacacaggc
    34141 actttgtcac caatctgaaa ccagcgtcat aaaaagccga ttgctactcg acaagacgtc
    34201 cgttacccga gaattttgct ggcttcgcgc gctacattat ccatctcgtc caacagttcc
    34261 agcagctccg gctccaaatc ttcttcttta gtaccgctac gtagctgttg ttcgatcagt
    34321 tggcagagat tcttcatacg cggcacaccg ctatagccgc aactgccatg cagtttatga
    34381 atcaaatcaa ccaggccttc cgggttttct ccaaccagct gttcctcaac tttgttgcga
    34441 acttcaggca ggaaatcgag taacatttgc agcatatcgc gcgctaaatc ggtttttcct
    34501 gctgcctggc gtagtgccag ttgccagtcg agggtcgcat tcgggttcac cacaatttcg
    34561 ttgacttcgg gcgtcacgac gcgagaggaa ataccgctgc caggcttgta gcgcaacaac
    34621 aaattatgca atcgctcttc ttcaatcggt ttcgccagat aatcgctcat ccctgcgcca
    34681 agcagcttct ctttttgccc ggccattgca tgcgccgtta ccgcgataac cggcgtttgt
    34741 tgctgatggg ggagctggtg gatgagctcg caggcccgaa tgccatccat gtcaggcatt
    34801 tgaatatcca ttaagatcaa atcgaacggc atctgtttcg cccgttcaac cgcctgatgc
    34861 ccgctatcgc aaagttccac atgttgcacc atatcttcca gcaatgcgcc gataagtttc
    34921 aggttagcgg ggttgtcatc aactgccatg actgtcattg ccagcttgct ttcatcggtt
    34981 acaggcaaaa gcgtgttttg tttgtgatga caaaattccg tcagggcagg caacaggcgc
    35041 gtaggtgtta atggtttcag cagacacgcg ccgataccat cttgcttgag tttttctgca
    35101 ttgacttgtg catggcaagg aagtgccagc atcaggaaat cggtcatcga taccgctttc
    35161 gctaatcgct catgttgcat tgttagcggc tcgcggaagg tcaccgcgat gcctaacaac
    35221 atcatgtcgt aatgcgcggg aggcagcgcg gagaacgttg ggctataaac cacttccagc
    35281 ggcgtttcac ttaaaatatc cagcgtgcat tgcgctgctg cggagtttgg ttcgacatag
    35341 gccaggcgct taccggcgag gcactgggtg gatggccctt cgataataat gttcgggttc
    35401 agatcgagat taatgtggaa ccagaaagtt gaaccgcgat tcggctggct atggaacgaa
    35461 atatcgccgc ccatttcatt aaccagtttt tgtgtaatca ccagccccag accggtgcca
    35521 ccatgacggc gggaaatact ggcatcagcc tgtcggaagg cctggaataa gcgcgattga
    35581 tcgcgttcag gaataccaat gccggtatcc cgaatctgca cttcaatctg cactttggta
    35641 ttactcagcg cacgtttttc taccagaata tcaatgttgc cattctcggt gaatttaatt
    35701 gcattcccca ccaggttagt gatgatttgc tgtaatcgca gtgggtcgcc gatcacgtta
    35761 tcaggcacgt cgcttttaat attgagcgtc agttccaacc ctttatcgtg agaagaatgt
    35821 gccagcagag taacgacttc atccagcgtg ctgcgtagtg ggaatggaat actttccaga
    35881 atcagcttac ctgcttccag tttcgagaag tcgagaacat cattaataat tgccagcaaa
    35941 ttatttgccg aacgttcaat cgtattcagg tgatcgcgct gcgttggtgt taattctgtt
    36001 ttcagcgtca ggcgggtaaa gccaataaca ccattcagtg gtgtacgcag ctcgtgtgac
    36061 atatttgcca gaaactcgga tttaatacgc gccgcttcct gggcgcgctt tttcgccaga
    36121 tctaactcaa cgttctgaat ttccatctgc tccagcgttt cacgcagatc ggacgtcgcc
    36181 tggtcgatat tgtgctgcat ctcttcgtga taagcagcca gcgacattgc catcgagttg
    36241 ataccgtttt tcagcatatc cagctcgccg agcataaatc cttccacccg gctgtcgagt
    36301 tgcccgcgac ggatgcggtc gacggtattc accatgttgc gaatcggacc ggttacatcg
    36361 cgcattaagc gccagccaaa aataagcgca ataccgatac aaaacagcat catcacgctg
    36421 gaaataaaga tctctttata ttgctgcaag cgaaccgatt taagatccag ctccagcgca
    36481 atatatccca acatattttg actatttttg gcatcgctac tgggcgattc gtcgggggag
    36541 taactctcag aaataatcgg cgtgcggagg atcataatat cgccgtcacg agtgacagtg
    36601 agctggcgag gaaacggcac gttgctgccg agctgcattg atgagggatc aagatgaaaa
    36661 ttggaggtga caaagagtcg gttattttca tcataaaccg aaatcgcgcg aacaatatcg
    36721 gaatggcgac gatgcagtac gcttattaac tgaccgatag attcgcgatt ttgcaggctc
    36781 atgccatatt cagtagaaac tgcaagcggc tcaataatgc tggcaccggc atcttccagt
    36841 tgacgctgca agtcgttata gcgatgcacg acgaaaaaga tactcagcaa taaaccaata
    36901 aggacggtcg gtgccaggat cagaatcatc atgcgtgcgc gcaggctgta gttggtcatg
    36961 gagttccgtt atgggacaat taaggtcaca ctgttaaatt gagaaagtct cgccaatggc
    37021 gcaattctac tctgcaaaac gacgcacgac gacgcgtcag atcataaccg tttcagtcaa
    37081 cgacctcgac tcttttggtc agggcgtggc gcgacataac ggcaaaacgt tatttatccc
    37141 cggattattg ccgcaggaaa acgcggaagt tgctgttact gaagataaaa aacagtatgc
    37201 ccgcgctaaa gtcgtacgcc ggttaagcga tagcccggaa cgcgaaacac cacgctgtcc
    37261 tcattttggc gtatgcggcg gctgtcagca acaacacgcc agcgtggatt tacagcagcg
    37321 aagcaaaagt gcggcactcg cccgattaat gaaacacgat gtctctgaag tgatcgccga
    37381 tgttccctgg ggctatcgcc gccgcgcgcg tttaagtctg aactacttac cgaaaacaca
    37441 gcaacttcag atggggtttc gcaaagcggg ctccagtgac attgtcgacg ttaaacaatg
    37501 ccccatttta gtgccccaac ttgaagcatt gctgcccaaa gtcagggcat gcctgggcag
    37561 cttacaagtt atgcgccatc ttggtcatgt tgaactggta caggcaacca gcggcacgct
    37621 gatgattttg cgccataccg caccgctaag ttcggcagat cgcgaaaaac tggaacgctt
    37681 ttcgcattct gaaggcctgg atctgtatct cgcccccgat agtgagatac tcgaaaccgt
    37741 ctctggtgag atgccctggt atgactcaaa cgggttgcgc ttaactttta gcccgcgcga
    37801 ttttattcag gtcaatgcgg gtgtgaacca aaaaatggta gcgcgtgcgt tggaatggct
    37861 ggatgtgcaa cctgaagatc gcgtactgga tctgttctgc ggtatgggca actttacact
    37921 gccattggcg aaacaagcag ccagtgtggt cggtgtagaa ggtgttccgg cgctggtgga
    37981 aaaaggccag cagaatgcgc gtcttaatgg tttacagaat gtgacgtttt atcacgaaaa
    38041 tcttgaagaa gatgtcacaa agcagccgtg ggcgaaaaac ggcttcgata aagtgttgct
    38101 ggacccggcg cgagcaggtg ccgcaggtgt tatgcagcaa attataaaac tggaacctat
    38161 tcgtatagtt tatgtatcct gtaaccctgc aacgctggct cgggatagcg aagcgttatt
    38221 aaaagcagga tataccattg cgcgactggc gatgctggat atgttcccac acacgggaca
    38281 tctggaatcg atggtacttt tctcgcgcgt taaatagtta cgatttgccg atttcggcag
    38341 gtctggtccc taaaggagag gacaatggtt gcggtaagaa gtgcacatat caataaggct
    38401 ggtgaatttg atccggaaaa atggatcgca agtctgggta ttaccagcca gaagtcgtgt
    38461 gagtgcttag ccgaaacctg ggcgtattgt ctgcaacaga cgcaggggca tccggatgcc
    38521 agtctgttat tgtggcgtgg tgttgagatg gtggagatcc tctcgacatt aagtatggac
    38581 attgacacgc tgcgggcggc gctgctgttc cctctggctg atgccaacgt agtcagcgaa
    38641 gatgtgctgc gtgagagcgt cggtaagtcg gtcgttaacc ttattcacgg cgtgcgtgat
    38701 atggcggcga tccgccagct gaaagcgacg cacactgatt ctgtttcctc cgaacaggtc
    38761 gataacgttc gccggatgtt attggcgatg gtcgatgatt ttcgctgcgt ggtcatcaaa
    38821 ctggcggagc gtattgctca tctgcgcgaa gtaaaagatg cgccggaaga tgaacgcgta
    38881 ctggcggcaa aagagtgcac caatatctac gcgccgttgg cgaaccgtct tgggattggg
    38941 caactgaaat gggagctgga agattactgc ttccgttatc tccacccgac cgaatacaaa
    39001 cgcatcgcaa aactgctgca tgaacgccgt ctcgaccgcg aacactatat cgaagagttt
    39061 gtcggccatc tgcgcgctga gatgaaagct gaaggcgtta aagctgaagt gtatggtcgt
    39121 ccgaaacaca tctacagtat ctggcgcaaa atgcagaaaa agaacctcgc cttcgatgag
    39181 ctgtttgatg tgcgtgcggt acgtattgtc gccgagcgtt tacaggattg ttatgccgca
    39241 ctggggatag tgcacactca ctatcgccac ctgccggatg agtttgacga ttatgtcgct
    39301 aacccgaaac caaacggtta tcagtctatt cataccgtgg ttctggggcc aggtggcaaa
    39361 accgttgaga tccaaatccg caccaaacag atgcatgaag atgcagagtt gggtgttgct
    39421 gcgcactgga aatataaaga gggcgcggca gctggcggcg cacgttcggg acatgaagac
    39481 cggattgcct ggctgcgtaa actgattgcg tggcaggaag agatggctga ttccggcgaa
    39541 atgctcgacg aagtacgcag ccaggtcttt gacgaccggg tgtatgtctt tacgccgaaa
    39601 ggtgatgtcg ttgatttgcc tgcgggatca acgccgctgg acttcgctta ccacatccac
    39661 agtgatgtcg gacaccgctg catcggggca aaaattggcg ggcgcattgt gccgttcacc
    39721 taccagctgc agatgggcga ccagattgaa attatcaccc agaaacagcc gaaccccagc
    39781 cgtgactggt taaacccaaa cctcggttac gtcacaacca gccgtgggcg ttcgaaaatt
    39841 cacgcctggt tccgtaaaca ggaccgtgac aaaaacattc tggctgggcg gcaaatcctt
    39901 gacgacgagc tggaacatct ggggatcagc ctgaaagaag cagaaaaaca tctgctgccg
    39961 cgttacaact ttaatgatgt cgacgagttg ctggcggcga ttggtggcgg ggatatccgt
    40021 ctcaatcaga tggtgaactt cctgcaatcg caatttaata agccgagtgc cgaagagcag
    40081 gacgccgccg cgctgaagca acttcagcaa aaaagctaca cgccgcaaaa ccgcagtaaa
    40141 gataacggtc gcgtggtggt cgaaggtgtt ggcaacctga tgcaccacat cgcgcgctgc
    40201 tgccagccga ttcctggaga tgagattgtc ggcttcatta cccaggggcg cggtatttca
    40261 gtacaccgcg ccgattgcga acaactggcg gaactgcgct cccatgcgcc agaacgcatt
    40321 gttgacgcgg tatggggtga gagctactcc gccggatatt cgctggtggt ccgcgtggtg
    40381 gctaatgatc gtagtgggtt gttacgtgat atcacgacca ttctcgccaa cgagaaggtg
    40441 aacgtgcttg gcgttgccag ccgtagcgac accaaacagc aactggcgac catcgacatg
    40501 accattgaga tttacaacct gcaagtgctg gggcgcgtgc tgggtaaact caaccaggtg
    40561 ccggatgtta tcgacgcgcg tcggttgcac gggagttagg ccgaaatttg ctcgtatcta
    40621 caatgtagat tgatatatac tgtatctaca tatgatagcg gtttgaggaa agggttatga
    40681 tccacagtag cgtaaagcgt tggggaaatt caccggcggt gcggatcccg gctacgttaa
    40741 tgcaggcgct caatctgaat attgatgatg aagtgaagat tgacctggtg gatggcaaat
    40801 taattattga gccagtgcgt aaagagcccg tatttacgct tgctgaactg gtcaacgaca
    40861 tcacgccgga aaacctccac gagaatatcg actggggaga gccgaaagat aaggaagtct
    40921 ggtaatggta agccgatacg tacccgatat gggcgatctg atttgggttg attttgaccc
    40981 gacaaaaggt agcgagcaag ctggacatcg tccagctgtt gtcctgagtc ctttcatgta
    41041 caacaacaaa acaggtatgt gtctgtgtgt tccttataca acgcaatcaa aaggatatcc
    41101 gttcgaagtt gttttatccg gtcaggaacg tgatggcgta gcgttagctg atcaggtaaa
    41161 aagtatcgcc tggcgggcaa gaggagcaac gaagaaagga acagttgccc cagaggaatt
    41221 acaactcatt aaagccaaaa ttaacgtact gattgggtag tgttactaac tcacttctat
    41281 tctggtcaca ggtttccacc tgacagaccc gcaattttca ggacaattca atgaatcaaa
    41341 tcgaccgttt gctcactatt atgcagcgcc tgcgcgatcc ggaaaacggc tgcccgtggg
    41401 ataaagagca gacatttgcc accattgcgc cttacaccct tgaagaaacc tacgaagtgc
    41461 tggacgccat cgcccgtgaa gattttgacg atcttcgcgg tgaactgggc gatctgctat
    41521 tccaggtggt gttttacgcg caaatggctc aggaagaagg gcgctttgac tttaatgata
    41581 tttgcgctgc tattagcgat aaattagagc gtcgccatcc gcatgttttt gctgatagtt
    41641 ctgccgaaaa cagtagtgaa gtgcttgccc gttgggagca aatcaaaacc gaagagcgcg
    41701 cgcagaaagc gcagcattcg gcgctggacg atattcctcg tagtttaccg gctttaatgc
    41761 gtgcgcaaaa aatccagaaa cgttgcgcca acgttggctt cgattggacg acgcttggtc
    41821 cggtagtcga taaagtctac gaagagatcg acgaggtgat gtacgaagcg cggcaggctg
    41881 ttgtcgacca ggctaaactg gaggaggaaa tgggggacct gctgtttgcc acggtcaatc
    41941 tggctcgtca tttagggaca aaagcggaaa tcgcattgca aaaagcgaac gaaaaatttg
    42001 agcgtcgttt tcgcgaagtg gagcgtattg ttgccgcgcg tggactggaa atgacaggtg
    42061 ttgacctcga aacaatggaa gaagtctggc aacaggtaaa acggcaggaa attgatctct
    42121 aagggaatta cgcggtcaag cgccatttgt gtcatttttt aaatgacaag cgcttgattt
    42181 gcgtcaaaaa catttacccc aaaggggcta ttttctcact cctgatttca atagtgcgct
    42241 ggcgaagagg agggataatg aaagtttgtg gcacaggtca tgttcgggta tactgctttc
    42301 ccgtcctggt tattccatcg tcttttcaac ctaacttctc aggttcagca tgacaacgaa
    42361 ctatattttt gtgaccggcg gggtcgtatc ctctctgggt aaaggcattg ccgcagcctc
    42421 cctcgcagcc attcttgaag cccgtggcct caatgtgacc atcatgaaac tggatccgta
    42481 catcaacgtc gatccaggta ctatgagccc aatccaacac ggggaagtgt tcgttactga
    42541 agacggcgct gaaaccgacc tggacctggg gcactacgag cgtttcattc gtaccaaaat
    42601 gagccgccgc aacaacttca ccacgggtcg tatctactct gacgttctgc gtaaagaacg
    42661 ccgcggtgac tacctcggcg caaccgtgca ggttattccg cacatcacta acgcaatcaa
    42721 agagcgcgtg ctggaaggtg gcgaaggtca tgacgtagta ctggtagaaa tcggcggtac
    42781 agtaggtgat atcgaatcct tgccgttcct cgaagcgatt cgccagatgg ctgttgaaat
    42841 tggccgtgag cacactctgt ttatgcacct gacgctggtg ccgtacatgg cagcgtctgg
    42901 tgaagtcaaa accaaaccga ctcagcactc tgtaaaagag ctgctctcca tcggtatcca
    42961 gcctgacatc ctgatttgtc gttcagatcg cgctgttccg gcgaacgaac gtgcgaagat
    43021 tgcattgttc tgtaatgttc cggaaaaagc ggttatttct ctgaaagacg tcgattccat
    43081 ctataaaatt ccgggcctgt tgaaatctca ggggctggac gattatattt gtaaacgatt
    43141 cagcttaaac tgcccggaag cgaatctgtc cgaatgggaa caggttatct tcgaagaagc
    43201 gaacccggta agtgaagtca ccatcggtat ggtcggcaag tacattgaac tgccggatgc
    43261 ttataaatca gtgatcgaag cactgaaaca cggtgggctg aagaatcgtg tcagcgtcaa
    43321 catcaaactg atcgattcac aagatgttga aacgcgcggc gttgaaatcc ttaaaggtct
    43381 ggacgcaatc ctcgtacctg gcggtttcgg ctatcgtggc gtagaaggca tgattacgac
    43441 cgcgcgtttt gcgcgtgaga acaatattcc ttatctgggc atttgcctgg gtatgcaggt
    43501 ggcgttaatt gattacgctc gccatgttgc caacatggag aacgccaact ctacggaatt
    43561 tgtgccagac tgtaagtacc cggttgtggc gctgattacc gagtggcgcg atgaaaacgg
    43621 caacgttgaa gttcgtagcg agaagagcga tctcggcggt accatgcgtc tcggcgcaca
    43681 gcagtgccag ttggttgacg atagcctggt tcgccagctg tacaatgcgc cgacaattgt
    43741 tgagcgtcat cgtcaccgtt acgaagtcaa caacatgctg ttgaaacaga ttgaagatgc
    43801 aggtctgcgc gttgcgggcc gttccgggga tgatcagttg gtcgagatca tcgaagttcc
    43861 gaatcacccg tggttcgtgg cttgccagtt ccatccggag tttacttcta ctccacgtga
    43921 tggtcacccg ctgtttgcag gctttgtgaa agccgccagc gagttccaga aacgtcaggc
    43981 gaagtaagta aaaaagttag aacggcaacg cgtaccctgg gtacgcgttg tttgtctgga
    44041 gtttcagttt aactagtgac ttgaggaaaa cctaatgtcc aaaatcgtaa aaatcatcgg
    44101 tcgtgaaatc atcgactccc gtggtaaccc gactgttgaa gccgaagtac atctggaggg
    44161 tggtttcgtc ggtatggcag ctgctccgtc aggtgcttct actggttccc gtgaagctct
    44221 ggaactgcgc gatggcgaca aatcccgttt cctgggtaaa ggcgtaacca aagctgttgc
    44281 tgcggtaaac ggcccgatcg ctcaggcgct gattggcaaa gatgctaaag atcaggctgg
    44341 cattgacaag atcatgatcg acctggacgg caccgaaaac aaatccaaat tcggcgcgaa
    44401 cgcaatcctg gctgtatctc tggctaacgc caaagctgct gcagctgcta aaggtatgcc
    44461 gctgtacgag cacatcgctg aactgaacgg tactccgggc aaatactcta tgccggttcc
    44521 gatgatgaac atcatcaacg gtggtgagca cgctgacaac aacgttgata tccaggaatt
    44581 catgattcag ccggttggcg cgaaaactgt gaaagaagcc atccgcatgg gttctgaagt
    44641 tttccatcac ctggcaaaag ttctgaaagc gaaaggcatg aacactgcag ttggtgacga
    44701 aggtggctat gcgccgaacc tgggttccaa cgctgaagct ctggctgtta tcgctgaagc
    44761 tgttaaagct gctggttatg aactgggcaa agacatcact ctggcgatgg actgcgcagc
    44821 ttctgaattc tacaaagacg gtaaatacgt tctggctggc gaaggcaaca aagcgttcac
    44881 ctctgaagaa ttcactcact tcctggaaga actgaccaaa cagtacccga tcgtttctat
    44941 cgaagacggt ctggacgaat ctgactggga cggtttcgca taccagacca aagttctggg
    45001 cgacaaaatc cagctggttg gtgacgacct gttcgtaacc aacaccaaga tcctgaaaga
    45061 aggtatcgaa aaaggtatcg ctaactccat cctgatcaaa ttcaaccaga tcggttctct
    45121 gaccgaaact ctggctgcaa tcaagatggc gaaagatgct ggctacactg cagttatctc
    45181 tcaccgttct ggcgaaactg aagacgctac catcgctgac ctggctgttg gtactgctgc
    45241 aggccagatc aaaactggtt ctatgagccg ttctgaccgt gttgctaaat acaaccagct
    45301 gattcgtatc gaagaagctc tgggcgaaaa agcaccgtac aacggtcgta aagagatcaa
    45361 aggccaggca taagactgac tttatctgat ttaaaaatgc cagcctccgg gctggcattt
    45421 tttatggttg agttaccagc ggccggatgc gccgccacct ccagaagaac cgccaccgcc
    45481 gctaaatccg ccgccacttg agcctccacc ggaaccgctg ttgtctgaat gataactttt
    45541 acctgatgct cttttctgaa gtacacataa acaggtaaag accataaata tggtgaaagt
    45601 aaaaaagaag gaaaatacag caatggaaaa aattttatcg gagaacagga ataaatctaa
    45661 aagatagatt gctgaaatat agactccact tttacatgcg cgacaaaaat tactgccttg
    45721 atgaaaaaac cagaaaggga agaacatcac tgcaaatacc caaaaaatag caaagtaata
    45781 atgatcactg gaagacgctg attcactttc tgaaggatta gtcggatatt ggtgttgtga
    45841 agtgagttgg ttattcaaag cgtttatagc tagctctaat ccctgagcaa atttttgttg
    45901 tttaaatgcg ggtatcatgt tgctacggat gatatctcca gccagagcat cggttacctt
    45961 ttcttccagc ccataaccta cctggatgcg gacagtacga tctgaccagg caacaatgat
    46021 cagtatccca tcattacgtt tggcatctcc taaacgccaa ttgtcaaaaa ctcttgtagc
    46081 atattgttca attgtctcgt ctttggttgt gggaacaatt aatacagcaa tttgcacttg
    46141 ggtattattg aaaatagatt ctaacttgtg cgtcagttct ttcttttctt gtgagtttaa
    46201 gctactcgta agatccgtaa cttgttgctg taattgtggg acaatagttg gttgggctgc
    46261 aacaaaggaa ca
//
LOCUS       DAZFJF010000019        45987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFJF010000019 DAZFJF010000000
VERSION     DAZFJF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.019"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(164..1618)
                     /gene="amn"
                     /locus_tag="ACHKAU_001289"
     CDS             complement(164..1618)
                     /gene="amn"
                     /locus_tag="ACHKAU_001289"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO5530395.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1720..3036)
                     /gene="shiA"
                     /locus_tag="ACHKAU_001290"
     CDS             complement(1720..3036)
                     /gene="shiA"
                     /locus_tag="ACHKAU_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO5530396.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            3351..4403
                     /locus_tag="ACHKAU_001291"
     CDS             3351..4403
                     /locus_tag="ACHKAU_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO5530397.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(4573..6695)
                     /locus_tag="ACHKAU_001292"
                     /pseudo
     CDS             complement(4573..6695)
                     /locus_tag="ACHKAU_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(6622..7371)
                     /locus_tag="ACHKAU_001293"
     CDS             complement(6622..7371)
                     /locus_tag="ACHKAU_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO5530398.1"
                     /translation="MSKKYQLLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLLVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQTSGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            complement(8021..10042)
                     /gene="fyuA"
                     /locus_tag="ACHKAU_001294"
     CDS             complement(8021..10042)
                     /gene="fyuA"
                     /locus_tag="ACHKAU_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO5530399.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            complement(10173..11738)
                     /gene="ybtE"
                     /locus_tag="ACHKAU_001295"
     CDS             complement(10173..11738)
                     /gene="ybtE"
                     /locus_tag="ACHKAU_001295"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO5530400.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            complement(11742..12545)
                     /gene="ybtT"
                     /locus_tag="ACHKAU_001296"
     CDS             complement(11742..12545)
                     /gene="ybtT"
                     /locus_tag="ACHKAU_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO5530401.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            complement(12542..13642)
                     /gene="ybtU"
                     /locus_tag="ACHKAU_001297"
     CDS             complement(12542..13642)
                     /gene="ybtU"
                     /locus_tag="ACHKAU_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO5530402.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            complement(13639..23117)
                     /gene="irp1"
                     /locus_tag="ACHKAU_001298"
                     /pseudo
     CDS             complement(13639..23117)
                     /gene="irp1"
                     /locus_tag="ACHKAU_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
     gene            complement(23205..29312)
                     /gene="irp2"
                     /locus_tag="ACHKAU_001299"
     CDS             complement(23205..29312)
                     /gene="irp2"
                     /locus_tag="ACHKAU_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO5530403.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            complement(29503..30462)
                     /gene="ybtA"
                     /locus_tag="ACHKAU_001300"
     CDS             complement(29503..30462)
                     /gene="ybtA"
                     /locus_tag="ACHKAU_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO5530404.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            30719..32431
                     /gene="ybtP"
                     /locus_tag="ACHKAU_001301"
     CDS             30719..32431
                     /gene="ybtP"
                     /locus_tag="ACHKAU_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO5530405.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            32418..34220
                     /gene="ybtQ"
                     /locus_tag="ACHKAU_001302"
     CDS             32418..34220
                     /gene="ybtQ"
                     /locus_tag="ACHKAU_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO5530406.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            34213..35493
                     /gene="ybtX"
                     /locus_tag="ACHKAU_001303"
     CDS             34213..35493
                     /gene="ybtX"
                     /locus_tag="ACHKAU_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO5530407.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSTLGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            35521..36825
                     /gene="ybtS"
                     /locus_tag="ACHKAU_001304"
     CDS             35521..36825
                     /gene="ybtS"
                     /locus_tag="ACHKAU_001304"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO5530408.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(37019..37934)
                     /locus_tag="ACHKAU_001305"
                     /pseudo
     CDS             complement(37019..37934)
                     /locus_tag="ACHKAU_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(38096..38171)
                     /locus_tag="ACHKAU_001306"
     tRNA            complement(38096..38171)
                     /locus_tag="ACHKAU_001306"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38136..38138),aa:Asn,seq:gtt)
     gene            complement(38272..39069)
                     /gene="mtfA"
                     /locus_tag="ACHKAU_001307"
     CDS             complement(38272..39069)
                     /gene="mtfA"
                     /locus_tag="ACHKAU_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO5530409.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFGAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            39163..39252
                     /locus_tag="ACHKAU_001308"
     tRNA            39163..39252
                     /locus_tag="ACHKAU_001308"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39197..39199,aa:Ser,seq:cga)
     gene            complement(39921..40571)
                     /gene="zinT"
                     /locus_tag="ACHKAU_001309"
     CDS             complement(39921..40571)
                     /gene="zinT"
                     /locus_tag="ACHKAU_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO5530410.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(40828..41463)
                     /gene="msrQ"
                     /locus_tag="ACHKAU_001310"
     CDS             complement(40828..41463)
                     /gene="msrQ"
                     /locus_tag="ACHKAU_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO5530411.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(41464..42468)
                     /gene="msrP"
                     /locus_tag="ACHKAU_001311"
     CDS             complement(41464..42468)
                     /gene="msrP"
                     /locus_tag="ACHKAU_001311"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO5530412.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(42577..42990)
                     /gene="hiuH"
                     /locus_tag="ACHKAU_001312"
     CDS             complement(42577..42990)
                     /gene="hiuH"
                     /locus_tag="ACHKAU_001312"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO5530413.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            43123..43794
                     /gene="hprR"
                     /locus_tag="ACHKAU_001313"
     CDS             43123..43794
                     /gene="hprR"
                     /locus_tag="ACHKAU_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO5530414.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            43794..45152
                     /gene="hprS"
                     /locus_tag="ACHKAU_001314"
     CDS             43794..45152
                     /gene="hprS"
                     /locus_tag="ACHKAU_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO5530415.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(45260..>45987)
                     /gene="hchA"
                     /locus_tag="ACHKAU_001315"
     CDS             complement(45260..>45987)
                     /gene="hchA"
                     /locus_tag="ACHKAU_001315"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO5530416.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
BASE COUNT        10496 a        11887 c        12828 g        10776 t
ORIGIN      
        1 tgttttgaaa gcatacttgc ttttaatatg tggaaatcat taagacgata ataaaactcg
       61 tatccgccca aggaagcaaa cagcccgcgt ttattgcggg ctatttgcca gatgtcttac
      121 ttaatccttc acatctattt ttgataagaa ggttcagaac ttattatcgg aacggcggct
      181 cattaaaggt tcgtaattta cgtgaatgca gtcggtcgcc ttctgcgcgc agcaaatcga
      241 ttgcccgaat gccaatttgc aggtgttcgg aaatagcgcc ttcataaaaa cggttagcct
      301 ggccgggaag tttaatctcg ccatgcaacg gtttatctga aacacacagt agtgtcccgt
      361 atggcacgcg gaaacgatat ccttgcgcgg caatggttgc actttccata tcaattgcca
      421 cggcccggct taagttaaaa cgaagtgcag aagctgagta acgtaattcc cagttcctgt
      481 catctgtggt taccacagta cctgtacgca gccgctgttt gacttcctca ccgggcctgc
      541 cgctgactaa cttggtagcg tcataaagcg cacgttgcac ttcagcaatg cttggaatag
      601 gaatatcggg cggcagaacc gcatcaagaa cgtgatcatc gcgtaaataa gcatgtgcaa
      661 gtacataatc gccaatggcc tggctttcac gtaagccacc acagtgacca atcatcaacc
      721 agacatccgg gcgtagtact gccagatgat cgcagatggt tttagcattt gacggtccca
      781 cgccaatatt caccagagta ataccctgac catcggcggt aattaaatgc cacgcgggca
      841 tctggtgttt tttccatgca aggtcggaaa tggcttcttc tggcgcttcg gtttcggcgg
      901 tgatccagtt cccgccagca caagaaaggg caatgtaggg actatcagga tcgaggatct
      961 ggctgcatcc ccaacgaacg aattcatcca cataacgtgt gtagttggta aacaagacga
     1021 acggctgaaa atgttcaact ggcgtaccgg tataatggcg caaccgcgcg agggaaaaat
     1081 cgacgcggcg cgcatcaaaa tgcgatagcg gggagaattc agttggatga taaatgccgt
     1141 ctgcagtttc atcgccaatt tgcgccagtt ctgttgtcgg gaaatagcga gttaacccag
     1201 cgctcattga gcgatcaagt gtcaattcag agccatcaat gacataagga taagggattt
     1261 catgctgcga gggttgcacc gagatatgcg caccataatc ctgatacagc aacgtaagtt
     1321 gttcattgag atacgaacga aagagagtag ggcgagtaat cgtggtggtg tagctgcctg
     1381 cgtgagtaaa acgaccaaat gcgcgcgttt tagggggatt ggttgtgcta ccgtcccagg
     1441 ttacggtcag tgatggatag acaaaaagac cttgtttgcg ggcgttttca tcaggtaatt
     1501 cgccacttgt aatatagttg ccaatggcgt tgcgtaaagc aactacagat tgctcataca
     1561 gcgcgtcgag tttatccagt gcctgagctg gggtcagacc ggagccctta ttattcatgt
     1621 gtgtctcctg ttccatacaa ttaccgcaca tccgatagta tgtcacagga atgtgaaaca
     1681 aaagtgtaag gcgaagatcg gatgatcttc gccaggctat caagcgcgtt gattgtcttt
     1741 catcaacaaa gcggttattg cggaaatcag gcatccagcc agcaaataaa tggcgacgct
     1801 atgccagttc ccggcaaagt aagtgatgag tgcagcggca ataaaaggtg taaatccacc
     1861 accaaccaca ctggcaacct gataaccgac tccagcgcca ctatagcgat aactggcacc
     1921 aaacatttcg gtaaacatcg gttgttgcac acacaccacc atgtcatgcg caatgtttgc
     1981 cagcattatg gagaagaaaa ctatccagaa aatagattgt gcttcaagcg ccataaagaa
     2041 aggaaatgcg ctcaacattc cgattaacgc acctgtgata taaaccctgc ggcgaccaaa
     2101 acgatcggca agcctggcaa aacagggaat tgtcaggcag cttaatccac ctaccagcaa
     2161 accaatatta aggaaaagtt cgcgcggtaa ccccatattc tgggttgaat aattaagtgc
     2221 aaaggcggta acgatgtaca tcgtcagcaa ctcgcacagt cgtagcgcaa taatcttcag
     2281 gaaagcaccg ggatgtcgta acagagcttc gataaccggg atgcgttttt tcgcggccgc
     2341 ttgattatgt tgctgttgtt caaattccgc ggactcctcc atgccattgc gcacccacaa
     2401 tgctcccaga accagtacga tgctaaacag gaaaggaatg cgccagcccc agcttaaaaa
     2461 ctgtacgtca gtcgtcatca tactgatcaa tgaaaccagt ccggttgaaa gcagtaaacc
     2521 tactccgtag ccaacttgta caccgctact gtaaaaggct tttttatttt tcggcgcact
     2581 ttcaacggaa agcaacgctg cgcctcccca ttcgccgccg acggcaaatc cctgaatggc
     2641 acgcagtgtc accagcaaaa taggtgccca ccacccaatg gtcgagaatg aaggaagaat
     2701 accaatcaag gctgtcgcga tgcccatcat ccagacggtc agcattaaca tgcgcttacg
     2761 tcccagccgg tcaccaaagt gaccgaaaat gacaccgccg agcggtcgga aaaggaagcc
     2821 gacaccaaag gtggcaaatg cggcgagcgt tcccatcgcc gggcttactt gcgggaaaaa
     2881 ctcgcgatta aacaccagtg cggcggtaat gccatagagt aaaaaatcat accagtcgac
     2941 gacggcacca gcgaaactgc ctaacgcagc tcgtcgggcg cgacttaacg aaagcgtccc
     3001 ttcatcggga cgagtggaga tgagcgtgga gtccataatg atcctgtttg tactgtcttt
     3061 ttataattag tatggaaaag gtaactcaca ttgccgtcga tctataaacg aaccttatga
     3121 gactacctcg acagagcgca tctgcaaatg attttatggc tgattagcgg caacatgaaa
     3181 aattcgatgg aagataaaag cgcaggagtt gctggtgttg tttaaggatt aagcagaggg
     3241 cccacaggtt cgagatatga aacataaaag tccgcatatg cacaaaattg caccctgatg
     3301 attctgatag actgcattgc attataacca gtagggaggc gaggttagtt atgtttttag
     3361 tatcattgtt gagacgtatt gcatttagtt actacgatta taaagcttat aattttaata
     3421 ttgaaaaaac agactttgtt gtcatacata ttcccgatca gattggcgat gctatggcca
     3481 tctttcctgt tattcgggcg cttgaattgc ataaaattaa gcatctatta attgttacgt
     3541 cgacaattaa tttagaagtc tttaatgcgc tcaaacttga acagactaaa ttaacattag
     3601 tcacaatgac tatgcaggat cacgcaacat taaaagaaat aaaagattta gcgaagaata
     3661 taacactgca atacggtacg ccggatcttt gtatagaggc gatgcgtaaa aagaacctga
     3721 aaacgatgat atttatcagt cagttgaaag caaaaacgaa tttccaggtc gtcggtttaa
     3781 ccatgaagtg ctattcccct ttgtgtaaga acgcgtcccg gatggatcag aatctccgga
     3841 ctcctgtacc catgacatgg gcatttatga tgcgtgaggc gggttttccg gcagtcaggt
     3901 caatatatga attgccatta agcgatgatg tactcgatga ggtacgcgag gaaatgcgat
     3961 cgttaggatc ttacattgcg ctcaatttag acggtagctc gcaggaacgt acattttcat
     4021 tatcgattgc agaaaatcta atagcaaaaa ttcaaagtga aacagatatt ccaatagtga
     4081 tcgtttatgg acccaaaggg gaagataaag ccagggcatt agtggattgt tataataatg
     4141 tctaccgttt atccttatca ccctcgatta aacgttcagc agcaatcata aaagatgctt
     4201 atattgcaat aactcctgac acctcaatat tacatatggc aagtgcctat aatacccctg
     4261 ttgttgcaat ttatgctgat tacaaaacgc gatggcccgc aatggccgat gtttcggagt
     4321 cagtcgtcgt tgggcaaaaa attgacaata taagtctgga tgaattcgca aaggcattaa
     4381 aaagtgtttt ggcgagaatt tgaggcagta cttatgcata gtaatgccga tctgttatga
     4441 acggtggtgt catcaatact ctgaggctgg tcaatcatca cagaaaacca gcctttggat
     4501 caaagcaata ctcaccgaaa atgatcatag tcacaccgaa cgcgattcag gaggaactgt
     4561 ttggtaaata gcaccaccgg atcttggggg cgctgtgatt attactgtat tgctatcacc
     4621 gtcatttttg aatgtcactt taccagtagc atcaacgcct acccaggagg ccgagcttga
     4681 aaacgaataa tcagccgccg tttttcctgg tgcaaagttg tcattattca actgataata
     4741 cgccccggta aatccctgcg aagggaatga agctgtcggt aggtttgcac tattcactga
     4801 cactgtaccc gtcattggtc tggtttccgc gctaatgaat tctatcgtgg tactcaggcc
     4861 cgcctgatga acaccattca atacggggat cagcgttgcg ataccctctc cgtctcctgt
     4921 aacagtagct ttgtaatcac cattgatatt ttgactgtaa tcaattgcgc taattttcat
     4981 atagggcacg tttgtacctg attgcacaaa ttccatccct tcagaaactt tgatcggatt
     5041 gcctgatata tcgtgcagaa caagatgtag ctcagcacta tcggtaaagt cccctttcag
     5101 tgatgactgg ttgttcttaa ggatggactg cgaggcgtct gctgggcctg ccactagcga
     5161 tatatctact gtttgcattc cacctgcgct tatgactgcg cttacagtga cgctccctgt
     5221 tatcgctcat ctcacgtttg ttgtcgcgat tccgttttga tcggtcaccg ctgttaatga
     5281 cgttaatgtg gtggagccgc tttttaaggc gaagtacaca gtgagacctt cgatcaggtt
     5341 accactgcca tcctcaaccg ttgcctttaa ggtacttaaa tcactgttgg tggcggcgat
     5401 agtcgacgga tcagcgataa agctagcaac atgtgcggtg cttgggttgc cagtaacctt
     5461 cacggttgtc tgtgtctgta ttgtgacgcc gttatggaac gatgcagtca ccgtatatgt
     5521 accgacttta ttgctggtca gaacgactgg agcctgacct gaagagttgg ttctcacttt
     5581 tccgccactt aacgtcgcac cttctagcgt tacgctaaag ttgatgacct gaccctccac
     5641 tggagtgcca tttgcagagg ttagcgttgc cgtcagagta gcgcctttag gagcattgac
     5701 accgataagc ggactggacg tgagtgtcag tttttcatca atagcctcca gttgtttctc
     5761 aagtgaagct ccattcgcta gggatgcttt tacagtatac gagccgtttc tttcagacgt
     5821 catggtgacc tcggctactc cctgcgtatt agtagagacc gtgttctggc tgatgatcat
     5881 ttgcgaggat ggcgctgcac tgaatgtcgc ggcttgatgc gtcaccgggt tgccaaactg
     5941 gtcgttaacg tgagctttaa ctgctatgtc ttgtgcgacc atgacctgac cttcaggtat
     6001 ctcctgactg gtaatcgtcg cagaattaac atctaccttg gcattcagta atcgcgagat
     6061 gacttcagta ggtttatctg ccagagaggc tgaaactgtt tttagtccga cctcggtgct
     6121 tgtcacaaga atttcagcaa aaacctgatc atccgtttca acgctggtgc tgcttagcgt
     6181 gacggaggtt ccacggaaat taacttttat accttctact gggttgccgt ttccatcagt
     6241 cactattgct tgtagcctgg tcattccaat gttattggcg atgaaattgt cctcggtaac
     6301 gttaagatta acctgcgcag tgagcgtatc cgcagtaaag ttcaccacta actgctcact
     6361 cttaccgcca accatcgatg ctgtcacggt atgagcgcct gcttttgtac ctttcagcgt
     6421 gactttcgct ttgccatcgg catcagtaac cgctttgtcg ccatcgctca gcgtgaagtt
     6481 cgccttcaca tcttccggta gagtaaatgt tacctcagtg ttggctatcg cattgccctc
     6541 tgtatcagcg acggtgctgt tagtgtcgtg agatcgttat tgtcggcaat caccggatcc
     6601 ttcaaggctg ccagcgtgat ttcagcattc gtgacgttcg gcacatagtt cactgtttgt
     6661 tgcatcgaat cgccattttc gagggttgcc gtcacgggag ttcttcaggc tgaaatcttc
     6721 atccacgccc agcgtgattc ttgcggtacc gaagcggctt aaccagtctg tcattacccc
     6781 tgaagtctga gaagaggccc atccacgtgc tatgtttgcc gcttgctcgc tgttcatatc
     6841 ttcggcgagc agagatccaa ttagctgtga agtactggct atctgttgct caaggttgcc
     6901 actgctgtta cccggcggcg gggttaaatt tttttcacta acttgtgcct ggacatccag
     6961 ttcatcaccc gggcggacat tacgaacaaa cgtacgaaac tggttgagtt tgcgtaacag
     7021 gggggtatct ataatttgtt gcacaggaca aatatatctg gcttctgtac agagtcttaa
     7081 attttgacca taaataaaac cgtccctgtc aagcatgcat agtttatcaa gtaggtctcc
     7141 cacaaactgc ctgaatttta tttttccatt aaaagtttgt atcttcttaa gtatcacagg
     7201 agactggaaa tagaccaata gtaggatgtt gccactcaag gcatgacgat aaatatcgct
     7261 aggagtaatt ttttgtttta tggctttggt agtgatctca actgcttccg ttatagtgac
     7321 ccatgttgac atataatgag ttataagcaa tagctgatat ttttttgaca taatattatc
     7381 tccttctaca caacaattcg ttccgttaag gaaaaagatt ggtgtcacaa gtatttattt
     7441 atgattaatc agagggttta tgacagagaa aaggatgtat atttctgagt aaacatgtac
     7501 aggcctatgg ggtgactatt taatgagtaa atgccgaatg attttcgggg tgatgattat
     7561 aacttgagga aaaatcccag cgtggtttga gtagtgtacc tgagtgatat ttgtgttatg
     7621 tatgcattga ttgcagcaga ataaatatag ttcaggctag tatagtttat ggtagctatg
     7681 agaatatata atctatttta cctataaaat aatttatatg tatggggttg ttttttaggt
     7741 tgtttgtctg tgcttttata ttctggagat gtgccttgta tataggattg tttgttggtt
     7801 gtatttttta atgttgcact gttgtgaatt tttattttga tatgattatt tctttagtat
     7861 aaggagagaa aggaacgaga aagttaaatc tatttatcct aatcgcaatt tcatttttta
     7921 aataatcact atggattaat attttttgcg actctgctga gttactggtt ttaactagtg
     7981 gtgtaaaagg gatacctttt cggtatccct tttacaataa tcagaagaaa tcaattcgcg
     8041 tattgatacc gacggtgcga cccatattga cctgcgcgac ggcgctgctg ccgttcatgt
     8101 agccataggt acggtaacga cggtcgaaca ggttatcgac atagacggaa atgttcatcc
     8161 gttcagtcgc ctgccagccc aggctgctgt ccagggtggc ataggtgcct tgccgcaact
     8221 ggttgtcgcc atcgaaataa tgcggcccga ccagattaac cgccagtcgg ggcatcagtg
     8281 cgccatagcg cgtatcaatc acgccgttca cgctgcttcc cgcgccataa cgtggtacga
     8341 acggcacccg gttaccgtga tacaactcac tgtcattggt gaattcggaa cggatcacgt
     8401 tgccattgat atcccatgac cagcctggcg caaaccgcca cttcgcttca agctcaacgc
     8461 cggtggcgtc ggctttaccc gcattgctta atgtctgcat cccgtccggg ccagagtaaa
     8521 gctgcatgtc tttggtgtgg gtataaaacg tcgcggcttg cagcgtgacg tcagcggttt
     8581 cgtagcgggt gccaagttca tagttgatgg atttctcggc gacgaacggt ttggcatcaa
     8641 gacccgcagt aggcacgatg ttgtacccgg aaggtttata tccctgggct acacgggtat
     8701 acactctcca gtcatcggtc agcatatagc ctgcggatag ctgcccgagc acctgatcgt
     8761 cattgctctt accctggtcg ccaaacgggt tgccgagcat gctgccgtga tattgtgtac
     8821 tggatttatc atgcgagaag cgcacgccgc cgccgatatc aaaacgatcg gttaaatgcc
     8881 aggtcaggtc actgtatgcg gccagcgttt cagcggtggt atagccggta ctgcttaaat
     8941 aaggcattgt cggcatgtcg taggctgaat ttaacttctc gcgggtgttc tgccggtaca
     9001 gcccaaacac catatcaacg gtacgcgcat cgcccagggt tgcggcgcgc agctcctgca
     9061 catcctgatt ccagcgctga ggcatattga cgattaacga accggaaggg aaggtgcgcg
     9121 aataatgctg ctgctgccag gcgctgatca ggttgaaaac ccagtcatcg gtggtgtatt
     9181 tcccactcag ggtttggctg tcagtgcagc gccgcatgta cgggtctggt gaaccatcgc
     9241 tgatcgacag cttacggccc ttaatatcat tccatcccac ataggcatcc tgggtggcgc
     9301 gagtacattc gcgtgaggcg gcaaagccca tttcccaggg ctgatcgtcc ggcgccagac
     9361 gcagtttcac attccctatg ctggcgcggg tgccgcctaa gtcatcgctt cccgtcgcgg
     9421 ggttaatcat gtcgccgtca tcaacctggc gtaacagggt gacgctgccg tacagcaggc
     9481 catcctcaat ggggccgctc aggttgaact tacttcgata actgtcgcgg ctactgacgc
     9541 cgccttcaat atagccgcgc ggcgtgctgt ccggctgctg ggtgacgatg ttgatgatcc
     9601 cgccctgagc gcttttgcca tataacgttc cctgtgggcc tcgcagcaac tccacgcttt
     9661 gcacatcggt aagcgcctgg atggtgttgg tggaaagctg agggacgcca tcgacataca
     9721 gggtgacggc ggggttatag aagtcctgcg ctgaagagac accgcgtagc gagatcgtcg
     9781 aaaaaagcat gttgccgcta ttttcaatat tgagcccggg caagactctg gggagtttgt
     9841 cgctggcggt gacgccggcg tcgcttaatt ccggcgcgct gacaacagta gacgagacgt
     9901 tgttggctga tgccgagcgg gaagattgtt tactggcggt aaccaccagc gtgctttcgt
     9961 cttgctgtga agtctgggca ttagcaatgg cggggagcaa taatcccccc aaggccagag
    10021 gataaagccg tgtcattttc attgttgtga gtccctgtaa gacggcgaaa cgccaaaaat
    10081 accgacaatg gtgaattgaa aatgataact atttctaata gtggtgctac ccgaaacggg
    10141 tttatttcaa cctgtttcgg gtcggtttgc gcttattggg cagaatggcg ataacggtcg
    10201 acggcgagag ccgtcaggcg ttttttgtct atcttgccga cggcggtgag cggccagtgg
    10261 tccagaaact cgatttggtc aggaattttc cacgcgctga gccccatacg ggtcagttgt
    10321 tgacgcaact gctgatagtc agttggcacc tgctgcgcga tgataaacgc gcaaatccgc
    10381 tcgccaagca gcgtgtccgg cgcggcgacc accgcgcaat cttgcacttc cgctaaacgc
    10441 agcagtgccg attccacttc agccgcggct attttttctc cggcgcggtt gatctgctct
    10501 tttatgcgtc cctcaacgtg caggttcccc acctcatcca gcctgacatt gtcgcctgtg
    10561 cggtaaaacc cttgcgcggt aaaggcctgc gcgttgtggg caggggcgcg gtaatagccc
    10621 gaaatggtat aagggccgcg cgttaacaat tgcccggttt cgcccggcgc gacgtcgttc
    10681 tcgtcttgat caacgatgca gatttcatcc agaggggaca acgggcgccc ctggctgtgg
    10741 agaatggtgg catgcggatc gtccagccgg gtaaaacaga gcaggccttc cgccataccg
    10801 aaaacctgtt gcagggtaca gtcaaaggtg gcgataacct gctcagcaag cgtcgggtcg
    10861 agccgggcgc cgcctgcctg aatgacgcgc agcgacgaaa ggtcgctgtc ttcccactcc
    10921 ctggcctgca cccataattg cgccagcgcc ggaaccaggg cgacgtgagt cactctttcc
    10981 tgcgcgatta aaggcatcac ctcatcacag ctggcgctgt cggtcagcac cacttttccg
    11041 ccgcaggcaa gcgttcccag aataccgggg caggccagcg gaaagttatg cgccaccggg
    11101 aggacggcga gatacacgct ctgttggctg atgccgcaca gttcagcaga agcgctgaag
    11161 ttatagctat agtcggcatg tcggcgcggg atgagtttgg gcgtgccggt tgtgccgcct
    11221 gagagcaaca acaacgcggt ggtggaaaca tcaggctgtc gctcaccgtg aagggagaag
    11281 agcggcgtaa aatcgtcgct catggtctct ccagcgacca gaacatgacg caagcaggcg
    11341 tgtttgtgtg ccatctgtcg ggccagctct gcgtggtttt ccccgtgaat aacgtaagcg
    11401 acgggttgcg ccagctcaat cagcgcgtcg atatccagcg cccgttgcga gggcatcgcc
    11461 agcacgggga taacgcccag tcttaacaag gcgaacagca gggtaacaaa cgcgttgtcg
    11521 ttgggaagct gcacaattac atgctccccc gaacgcaaac ccagtgatga cagacctgcg
    11581 gcaagttcgt caacttgtgc atcaagcgcg ctgtaggtta acgacccctt tgcatcaacg
    11641 acggcaattc gcgaggcata acgggccgcc cagtggcgca actgttcggc aatgggtaag
    11701 ggatactgtt caatcagaga ttcaaaggaa gaattcatgc gtcacctttc tgaagtactg
    11761 ggctgttttt gccacgcggt cattgcagaa aatgcgtggg gaaaatggcg gacaatctgc
    11821 gtaaaaaagg accgggcttg ttgaatggga tagaagtgat cgccgtcaat caccaccggg
    11881 cctgtaacgt ggctcagcca ctgacgccag gcatcgacct gctgccagga ggcttcgcga
    11941 tcgtggctgc cgcacaataa cagcgcaggc gtgcgcagcg gcggacagac gtcgggcgag
    12001 tcgtagtgat agctctcggt ggcgtaaaaa tcagcgcgca gaagaggaag aaacagcgac
    12061 attaattcct ggttttcccg cagttcagga gaacatccgc caatgtctat cagctcagcg
    12121 ataaagtcgg catcatcgcg atggctgagc tggcgttcag aatgcagatg cggggcatgg
    12181 cacccggaaa taatcagtcc ttgtggcgca agcccccgtt gctccagaag tcggcaggtt
    12241 tcaaacgcca cctgcgcccc catgctgtgc ccggcgagta aaagcggcgt gtcaggcgat
    12301 acggatgctt ccagctcgtt cgccagcagt gccgccagtt gtgtaatgct tctgagcggt
    12361 tccagatggc gaaggcgatc gcgccccggc caggtcacca gagaaagcgc gcaatcagcc
    12421 agttgctcag cttgccagtg gcgaaacgcg ctactgctgc cgccagcgaa agggcacatg
    12481 accagatgcg cagtattgcc gttccgggcg ggccacagcg ggatgcacat tgcagattgc
    12541 gtcacagcgc ctccttatca tcatcgttgt agaaaccggc taaccggtcg tggtgcggcg
    12601 gcgtcagacg tctgatctcc gcattgccgg tcttacgtaa aatctgctgc cataaacgtg
    12661 ataacgcgat ctgatgtacg ctttggcagg cctctggtgg atgttcgccc gccagattgc
    12721 tgcgcaactg gtgtagcaac cagctgacgc cctccggccc gacggtttcg cagcagtcgc
    12781 gccagctcag gggcgccgcg ctgcgcgtca gacccggcag atcgcgcagg atctccggtc
    12841 tgcgccagag actgtggggg ttctgctgat ggtctgcgac atacagactg gatgaccaga
    12901 tgacggggcc gtagctcgcc tccagcgaaa gatgaccttc cggccagccc agcagcaggc
    12961 ggtgcatgat aaggctatgc atatctggat cgtcaggatc gagatagcgc tgaagcagca
    13021 ggcaggcttc tccctccggc cagaagagtc tcagacagtg aaaatcgcta aagctgccaa
    13081 ccatgtcgca ctccacggcg gcggcatcaa cgcccagagc cagcaacaac aaatccagag
    13141 tggagtagag caactgtcgg ctggtggtgg cgtggactac cggcggcgtc ttagccaggc
    13201 agcgacgcag ctgctgcgca tcgcgaagcc atgtgcgtcc cgcgcgcgta tgcggataaa
    13261 acgtgtttac ccaatagcag cagccctgtt cctgcgccag cgtttgtaag gaactgatgt
    13321 catccgggtg gaggggatgc tcctgaatga cgtgtacgcc gcgcgtcaga aagtgtctgg
    13381 caagctgcgt accggttcct ccggcgacgg tcgaacgcac gacaatacag gcgatatccg
    13441 gcatcctggt tatctgttcc ggcgaggtat acagcggaat gccaaacgca tgagccagct
    13501 ctcttgaacg ggcgcttccc tgcgccagca ggccgaccag ttccagcccc tccgggggct
    13561 gcataaaggc attcaggtac atttcgccaa atttggcgcc cacaatcagt acgcgttgtt
    13621 ttggggaggc ggacggcatc ataacgtgtt ctccggttgc gttgaggttg cgcaaagcca
    13681 gcgcgtaatg tgttgcgcac aagcctgaac gtggggggct tccatcatga tttgccagtg
    13741 gctggcttca atcacagcgt cgtcggcgtt gtttatccag ccctgcaact cggtttctgc
    13801 tggcgtccag cgcgcgggtc tcccggcggc atacaccatg agacagggga ccggaacgct
    13861 ttcgcccggc gtatgctcag tcagtaaacg cagcagatgc gcgatgttgt cgagccacgt
    13921 ttctgccgct tgcagcgtca ggttttgcga caccataccg gccgttttag cgaggctgat
    13981 aaagtcggca agctggctgt cgggcgtctg ctggtcgaaa tgttcgggca gtgccagagg
    14041 cgtttctcct tcggctaaca ggcgcagcag ggccgcccgg ttttcgcaac agaaatcctg
    14101 tcggcacacg ggatcgatta acaccatccg aacctgcttg cctttggcgt acaggcgctg
    14161 tgcggcgccc gccgcaagaa acgcgccata cgaccaaccc gccagcacat aagggccgtg
    14221 aggctgctga cgacggatgc acccgacata ctcatcgatc atctggtcga gcgtagcaaa
    14281 gcgctgcggc gattttgctt gcaggccgaa aacgcgcctg ttcaacgcgc tagcgagcgg
    14341 cagccaggcg ctgatatcgc cgtctgaagc gtggaagaca aacagggttt cctcaccgtc
    14401 gccctggcaa aggggtatcg ggttgtcctc tcctgacgta gccgcctgta gatgggcgca
    14461 gaagtcgctc agcgtcgaat ggttgaacag atcctgaagg ttagccctgg caatccctct
    14521 ccggtttagc tgcgcgacca tccttgtcgc catcaggctg tcgccgccca gttcgaaaaa
    14581 gtcgtgatgc cttgccacgg ggcgagacag cagcgattgc cagagttcag ccacctgttt
    14641 ttccagcgcc gaattccccc cggcaactgg cgtgggtttg gctggctcag tcggcgcatc
    14701 agacggggca tgcagcggcg taaataacgc ttctctttgc cggatctgta aaatgggcag
    14761 accggtgcca aagcgctgct gtaaatagcg gctcacggct tttttatccg ggcgatttac
    14821 gccaggcgaa cgcgctacca gcagatgctg gcgcagcggc gacgattcct gcgcgggcca
    14881 cgccagctcg tttgcaaacc cggcctgaac gagaacctcc tgccatgcgg agcgggtgag
    14941 catcggtttc tcatcccggc ggcggaaatc gcggtatccg cttaatccct caataaagcc
    15001 cacgctcgcc agctggaata cgctgtttcg ctccgtcgct tcaacgatca gcaaacgtcc
    15061 gccggctttc agcaacagtt ttaatctgcg caacgtctgg acgacatggc tggcgtcgtg
    15121 gagcacattg acggcaacga tcaggtcgta acccgcctcc gggtgggcgg tgaaatccag
    15181 cggctggttg atgtcgaaca aggcataaga cacctgcgat tcatcatgga aacgggctct
    15241 ggcgtcattg aggaactgcg cggagacgtc cgtgaagtgg tacgactgcc gcgtggcctt
    15301 cagcaccggc gcggtagtgg ctgcggttcc ggcgccaacc tccagaatcc gttctgcgct
    15361 gcacaaggcg gcaatctgcg cggtatagcg attcagacag gcgctggcgg ggttgtcgcg
    15421 atacagcgcg tcggtaacgc gatgctgctc gttgaacagc aattccagcg gagaacactg
    15481 cccggagaag agggcgtcgt gccgggcgat gcaggtttcc agatactgcg ccagcgcctg
    15541 gctccattgg ctttgcgggc acgcttcctg aggctcagga atctcgctga gcggaatgcg
    15601 gcagcgccag ctttcacctt cgcggattaa ccactctctt tccgtcagac actgtagcca
    15661 ctggcgcagc agacgctggt gagacgcttg cgcggacagc gcctgtacta ttgcgctaaa
    15721 gcgatgggcg atctccggcg tggtaaacag gccgtgacga tttagcgtag ccgcgatccc
    15781 ctgtagcgca cgcgcctcca gccagcgcca ggtcgcctcc agctcatcaa gctgcgcctg
    15841 ctcaggcaac ggcagcgcca gttgggtaag ttcgtgctca tcaggcatcg ccgctgatgg
    15901 cgaaggatcg tcggccatca cgatatccag cgtcaacgcg ccctgttcgg ttatttccgc
    15961 tctggcctgc cggatgccgg ggtattcgag cgtcgcctgc tcaatatccc gcaggtcgta
    16021 gtccgcctgc gcgttcagcg gccagcgtcg gcggggaatg tgcccggcga tgccgctggc
    16081 gagagtcttc aggctttcag gggcggcgat gacggcttgc aggatggcgc aatagtcatt
    16141 aaacatcgcc tcggcagcgc cgggttccag cacgttgtcc atgcagtacc agctaaacat
    16201 caactcgccg tcgctctcca tgacctgatg atccagccag acctgcggcg tttgcgtgaa
    16261 tacatagcag ggttcgccga acagatggct catcgcctga tcgatagtca tgccttccag
    16321 cgtcatcccc agcatactgg taaacactac cggcatcagc ggttgacgtt gtgatccgcg
    16381 caggcggccc agctcacgga tcacctcaac accgttcatt tcactgtgcg ccatattttg
    16441 ccagaggcgc tgttgggtct gttgcatctg ctcttgcaac gtcactggcg ctgagaagtt
    16501 aaaatcaacc agcgtgacgg aggtaaaatc accaatcaac tggttgattt gcggatggat
    16561 cggctggcga ttgaagaacg tcaggttcag cgtaaatgtt gtggtacggc tccagcgctc
    16621 aagggtggcg gcaaacagcg tgagcagcgc ggcagacggt gtgacgcctt gctgctgcca
    16681 gcgctgtttc acggcctgcc attctgtctt gccgatcgtc gatttgaagg tggtgaagtg
    16741 tggcgtttcc gggggcgtct caaccatcgg cagctctggc gctaagggca gttgcggcag
    16801 tttttcctgc cagtaatccc aggcatcgtg ccatgccgat gtctttcggc gcgcctgttc
    16861 agccatcaca taatcacgga aggtaatagc gagcggcgcc agcgtttcac cgcgccagac
    16921 ctgcgccagg tcgtccatca tgactttaaa actctgcaca tcaaactgca aaaggtcgag
    16981 gttcatatgc agacggtaat ggcaatcgtc gatttcgctg accaccagct caaaaagagg
    17041 ccactggtcg gcaggcaaaa cgcgatagct cagttcatgc cgccgttttt ccagcgcgat
    17101 gcgctgttct tccggggaaa gcgcgcgcag atcgtcacgc gggatgtgat actccggcgt
    17161 tgtcgccagg attcgctgct gcccgtcggc atcaaccacc atacgcaaca tatcgtggcg
    17221 tgcgatgagc tggttccatg ctttctccag tatggctaga tcgaactcat cgtggcgttt
    17281 atcccactca aacaggacgt gacaggcgac gccgccatag ccaatgaggt gggttcgccc
    17341 cagccagtag gcgtgctgaa tgggcgtcaa agggaagggc gcataacgct cgtcggcgtc
    17401 gtgccgcaac acttccggct gcgaaacggc aggcgtcgcc tctggcttag aacagataag
    17461 ctgcgtgagt ccatgaggag acagatcctg ccacgcccgt tccgcattga tgcgtacgcc
    17521 caggtagtgc tgaatatcgc tactgagttc aaggaagagc agcgagtcca tgccgagttg
    17581 caacagatcc tggtttggat gcagtgacgc cggatcgctc agccttagct gaaccgcaat
    17641 ccgctttttc agccatgcca tcaccgctgt ttcatcgctc aggctgccgt taaaggcgtt
    17701 gtcatcagca ggaggaatga ccggcgttgc ggctttttct gtggcgctga tgttaaacag
    17761 agcctgttgt aacgggggca ttttgtcggt aaaaacgcgc atcgccagtc gccacggggc
    17821 gccgcgcatc accgcctgtt ccaggtgcca gcagccttcg gcatcgctta acgcgcccat
    17881 acctcggctg gcgagcgtcg ccagcatttc cggcgtggcc gcccgaccgc tttctcccca
    17941 tgcgccccag gcgacagaga gcgttttcgg cgcatcaagg gtggaaaact gctgggccag
    18001 cccgtccagg tagccgcagg acagcgcatg ggcgctctga cccggcgcgc cgagggtggc
    18061 ggcagcggaa gagtagagaa taagatagcg tccgtcgtgg ttgcgcaggg tttgcaacag
    18121 ctggcttgcc gcctgcgctt ttaccgcgaa aacggcagcc agctggtggt catcaagctc
    18181 ctgcaagggc gcgtcagcca atacgccagc ggcatgaatc gctccggcaa tgccgccgtt
    18241 ggccgccaga tcgtcaagaa ccgtggccag ttgcccggca tcgcccacat cacaacggca
    18301 gacgcgcgtc tgcccgccct ccacgtcgcg tagccatgac tcatccacgt gcgatgccag
    18361 cagggcgatg cgtcgcgccc ctttttctct gagccagttc acggcaaggc ggcctaatcc
    18421 gccaaacgct ccggtcacca gatgccagcg gctatcgccg gtaaacacgt ttgccggtaa
    18481 ttcagctgcg catcccgtat tgggcgccag tgaagggagc caaagggtgt caccccgtgc
    18541 ggcgagccag cgctgtgata gtgagactgc gctcaaccct tgatgcagcg tttcccacgg
    18601 ggtgttttcg gcgagatcga tggcggcaag caaccgttcc ggctgttcgt tggccgcgac
    18661 gcgaagcaag gcccatagcg cgtgatggga tgcagagagt gcttcatttt cctcgactcg
    18721 ccacgcgcgg cgagtaacca caatcaatcc ggctgcgctg gcggtgagcg ctgctatcac
    18781 tttctccgct aaggccagcg tatcctcgct ctcctcaaca atcatcagtc gtgaagaggc
    18841 gtgcggatcg tgaatgatgc cgtattgcgc cagctcgtcg ctgcgcgcaa gcgtaccggc
    18901 gctgaagcta aaggtaagcg gatggtcaat gctggcgacg ttgaggggcg tccagcgcca
    18961 ttgataatgc gtttgcggcg caggaagagg cgcggcgggc gatagcggca gccactcgcc
    19021 agcaggatga cgggcttcaa cgcgcatctg accgcgggaa aaggcttcgt tttgccagct
    19081 taagcgaacc ttccccagcc actccggggc ggcgacgggg ggcggttcca caggcagagc
    19141 gtcgccatga cgctgtgaaa gcagacgcca ggcctcctgc cagcgggcgt taaactgttc
    19201 gggctgacct gcgcaatcag accagtcggc gagataatca ccgttatccg tcagcgcctg
    19261 ccccaacacg ggttctgatg gcgcggtgaa tgttacggcg ctaaccgcct gaccgggcag
    19321 cgtggcgaga atgatatgtt cgctcatccc ggcggtcggg ctgccatcct gcggtagcca
    19381 cgccactttg ctgaatccgg cgtggcggca ctgttgttgc cactgagcgg tggtgaggaa
    19441 taactcacct tcgcgggcgt cgagatcctg tagcggaaga accagcgggc cgaaaacgaa
    19501 gtcaaacaga cgcattggct gggtgatttc gcgcatcagc aggcgcccgc ccggcttgag
    19561 cagggggcgc agattatcga gcgtgcggcc aatatggcgg gtggcgtgaa tcacgttcgc
    19621 tgccacgata agatcgtaag actgtgcctg gaaaccctga gactgcgcct ctttttcgag
    19681 atccagctcg ctatacttca caaaatcata gtcggcgaat ttctgctggg cgcgacgggt
    19741 gaacagcgcc gagatatcgg tgaaatggta ctccagtgcc ggaacgccgt tgagttccgg
    19801 cagcagccac gcggtggtgc cgccggttcc gccgccaact tcaagaatac gcaacgactg
    19861 gcggggctga cgcgtctgga caatgccgcg taataccccg gcggcgattt ggttgaaata
    19921 gcggccaaag ctgaattcct gatacagcac ttccacgccg tcggaggcgc tttgcgggaa
    19981 gataatcgcc accggttctt ccgcgccgct catcatttca tataaccgat cgccggcacg
    20041 ggcgatggtg tcgggaatag cctgaaaacc ttcacaataa ctggcaagtt ccgtcagcag
    20101 tgattcccgc tgttgatgtt caatggggcg ggcgcggacg tatcgcccgt cggtgcagcg
    20161 gtaatcgccg tcgaccacgc agttattcag caggcgctgg agtagctgct ggtagcgggg
    20221 cagcagacgt ccacggcgca tgatggtcat ggcgtccacg ccgttctcaa tggcatcgcc
    20281 ggtacagcgt tgtaccagtt gatcgacgta gatggcgtgc agtcgcgtgg cgcactgttt
    20341 aagcgcttcc aggcgaggga gatcgagcgc tgtcgcggcg cgactcgcca cctccagccc
    20401 ggcagacagc gctgcgtcgg caggctcgca ggccggggag acgcgctctt tccagtaacg
    20461 ttcagtatca aacggataac atggcgcagc gatacgttgt ccatcgcccg ccagcaggtc
    20521 ggcccacggt agggcgacgc cggcagcgta aagctggagc agggcctgat tgaggacatc
    20581 gctcgcctct ttgttacgcc gggcgctggc tatccagtat gcgttatcgc ggtattcgcg
    20641 ctgcccgcaa gcaactgggc atcgggcccc atctccagaa aaacgcgggc gccgagctga
    20701 tgcgccacct gaatactctg gataaaacgc accggctggc gcatgtgtcg gcgccagtaa
    20761 tccgcctggt tgagcgttga ctcatcaatg acgtcggcgg tgagcgtgga aataatcggt
    20821 atttgccccg gctccgcgtg cagtcccgcg caggcgtcct ggaaccgatc gagtatcggc
    20881 tccagtaaag cggagtgcgc cgcaccggtt acgctcaggc gacgatagtt aatgtcatgc
    20941 tgcgagagcg tggcgcaaaa taccgcgaga cgggcttccg gcccggaaaa taccgtatgt
    21001 tgcgtaccgt tgttggcggc gagatccagc tcaaactggc gagccagcgg catcagcgtg
    21061 tcttcgtctg caaataccgc caccatcgcg ccgcttgcgc actgctgcat tagcgcgccg
    21121 cgccgacaaa ccagtggcat gacctgttca atcgtatagt gtccgcagac aacggcagcg
    21181 gcaaattcac cgacggaatg cccaatggcg aagtctggct tcagtccttc agcacgccag
    21241 tgcgccgcca tcgcgatttc aaacgcgaca atcgccggct gcgcccaggc catattgtcc
    21301 agctgcgccg aatcggggtt aaacatcgct tcgcgcagtg atggcgtgag catttcgcta
    21361 caggcggaaa aacagcgatc cagcgtgtcg gcaaacgccg ttgagtgctg gtacatcgtt
    21421 tggcccatag tgtgccagtg cgagccctgg ccggtaaaca gccacacctg cttgccgctg
    21481 gcgccgtggc cgctgtaaac cagcgccccc gatttctcac cggcccaggc gctgagcgcc
    21541 tcggcggttt cacggtttaa tggcgccgcc aggcggaagg ggagatcgag acggcgcgcg
    21601 tgcagggctg tgaaggccag agagctggca tccgcattct ctctcagcgc cccggcataa
    21661 tccgtcgcca gccgccgcaa cgcgctgtcg ctggcggcgc tgagcagcag cgcggtactt
    21721 tttctgccgc tatccgtatt ggggaggcgc gcgttgagcg cgtcgggcag cgaggcgacg
    21781 atcatatggc agttggtgcc gccaatacca aaggaggaga cgcccgcata gcgcatttcg
    21841 tcctgccatg cctgtgccga caccggtatg gtaaaggggc tctcttcaag tttcagcgcc
    21901 gggttggggg tgtgaaaatt cagtaaggga ggaatttgcc cgcgactgac tgccagaacg
    21961 gttttcagca gtccggcaat gcccgccgcg gtatccagat ggcccatgtt acttttcacg
    22021 gaaccgagcg cacagcgctg atcctgcggg cgaggcgcat agacgttgcg taacgcttca
    22081 atttcaatcg cgtcgcccag cggtgtgccg gtgccgtggg tttcaatgta acctacctgc
    22141 ctgtcgtcga tggccgccag cattaacgcc tcttcgatga ctgcctgttg ccctgcgacg
    22201 gaaggggcgg tatagccgac ctttctgttg ccgtcgttgt tgaccgcgct ggagaggatc
    22261 accgagataa tcggatcgcc tgacagcagc gcgtctctca ggcgacgcag caccacgcag
    22321 ccgagaccgt taccggccca ggtgccctca gccgaggcgt caaagggacg acagtgacca
    22381 tcaggagaga aaatcattcc gggctggtag cggtagcctg cctgctgggg gaaagagagc
    22441 gccacgccgc cggcaaccgc catatcggat tcgccagcgc gcaggctttc acaggccaga
    22501 tgcacggcaa ccagcgagct ggagcaggcg gtctgtaccg ataacgccgg gccgtgcagg
    22561 ttgagtttgt acgcggcgcg ggtggcaata tagtctttat cattgcccat cagagattgc
    22621 agacctttta cctgcgcgac ttctgtcacg ttcaatgctt cgcgaccggg gtaggtactc
    22681 atccgggaag aggcgaaaac gccggtctta tgggggacgg cgccgggggc ataaccggca
    22741 tgttccagcg catgccagac cgcctgcaaa aacaggcgct gctgcgggtc catcgactcc
    22801 gcttcctgtc gcgaatagcc aaacagggtg gcgtcgaagc agtcggcgtt gtctaacacc
    22861 gaaccgatat tgacataatg aggatcgtca atgatggcgg catccagacc gacggccaga
    22921 agctcttcgc gcgtaaagcg tcggctgcat tcacgacctt ccagcagatt ttgccagaac
    22981 gtttcgccat ccggcgattc aggaaaatgg caggcgtagc cgataaccgc gacaggttcg
    23041 cagtccgggt agtgttgagc gatcgatgca tcaatggaat ctgctgtcgg cgcagaagag
    23101 aagcgcaagt tatccataat cggtcccgtt tcctaagatt cacaaagtca atggaacgcc
    23161 atgcgttcgc gatggcgttc cggggaaaat cagtttgctt cgcgctatat ccgccgctga
    23221 cgacggcgaa cagggcgagg tcgttgtgtt ttctccaccg tggcgacgcc ggccagataa
    23281 tcggccaggg cagcaggaga agggtgggtg aagaggtcaa gcaacgtcag ggtggaaaat
    23341 tcgtgacgtt gtaacaaaac gtgcagttgc accagattca gcgacgttgc gcccgcctca
    23401 aagaagtttt ctgcgggcgt gacagactcg ccaaccacct cgcggaaggc gtcgcaaatc
    23461 aggctgacgg tgctctcgtc cgccgcaatg tgtggctgag agtgctcagg gcatacgcgc
    23521 ggtgagcgct ctgcgcattg ccctgcgtgt ttaacgggag gcaccagcgc ggcgagcggc
    23581 agttgccagc cgctttcatc ctcggccatt cggtttagca atgcgcaata ctgttcaaac
    23641 tggcgctcca cctgcccggc aggaaacagc gcggcgacaa aatcccagtt aaagcgcagt
    23701 tcgccttcgg attcataaat ctggtgatcg agccagacct gcggcgtctg ggagatgccc
    23761 cagaccggtt tgagcagatt acgccgggcg aggaagttat cctgctcaaa gcccagcgcg
    23821 ctggtaaaga cgacgggcat cggaacggct ggcacgtttt gccgttgcgc cagttgacgc
    23881 atcacgcgga tggctgacac atcgcggtgg ttgaggttct ggctcagccg ctgctgtagt
    23941 gactgcgcgc tgtgcagcca gctttcgccg ggatgccagc tcagcagcat cagcgaggtg
    24001 aaatcgccca gaatctggtt gatttgcggg tgcagcggtc gcctgtcgaa aagcgtaagg
    24061 ttaagcgtga agtcaggctg tgcactccat gcagagagaa ccgttgacca caccgacaac
    24121 agtacggccg acggggtgag atgggcgtca gccgcccgtt ttttcagccg atgccagcgc
    24181 gtgctgtcca gtgcgccgtt caggcgggcg aagcgcggtg tttcaacctc ctgaggcaag
    24241 cagcgcagcg gcaacgcagg cgccggagga atatcatcaa gctgcgcctg ccaccatgcc
    24301 agagaatctg gattgggcga ctgtagcgag ggttgttgca gataatccct gaaggtgacg
    24361 ggcagcggcg gaagcagctg ttgcgggtag cggtagccgt gctccagctc cgccagcagg
    24421 atctgcatgc tcagaccgtc aagcaacagg ttatccagac acagccacag gcgggcaggc
    24481 atcccgtcca cgtatccgac ctggagatcg aataccggcc atacttcggg gttgagtacc
    24541 tgatgcgcca gtttttcgcg cacccgcaac gcctcttcag gcgtatggag ggtgtgtgcg
    24601 ggtatcaccc aggggggcgt ctgttcgagc acctgttgct gtccatcacg cacgatggcg
    24661 cgcagcatat cgtggcgggc gattaatcgg ttccagaccg tctccagccg ggtgaggtcc
    24721 agatcggcaa tttcaaattc aacaaagaaa tgtgagccga cgccgcccag ggcaaagccc
    24781 ggctgacgcc ccaccaggta agcctgctgc acgtcggtaa gcgcaaaggg ctggtagcga
    24841 tcttcagggg agtggacgaa tggttgttcg accgggacgt cggttttccg cagcgtggcg
    24901 gcaaaatccg ccagccgggg atgattaaac aggtcgctta attgcgcttc ataacctgcc
    24961 tgatgaagtt gcccggtcag acgggtcgcc agcaggctat cgccgccttg ctggaagaag
    25021 tcggtttctc tggtgacatt gccagttgat aagagttgct gccagagggc ggcaacctgt
    25081 ttttcaatgt cgccctgggg taaatccgct tcggccgggt tttccgcttc aggggtatga
    25141 cgacgcttca gcgcctggta gtcaattttc ccgttagctg tcagcggtaa ggcgtcgagg
    25201 aagacgatcc gttgcggcac catccacccc ggcagacgcc cggcgagggc ggcctgaagt
    25261 tgacggggat cgcggcgcac ctgcctgtca ggacattgta cgaggaagcg ttgtaacccc
    25321 gcgacctcat cgccatgctg acagttgaag ccggccgcgc taaataatgc cgcccagtcg
    25381 gcgctgttat gcagcaaggc ctccggctgc ccattggtta acaggagcgt gctgagtagg
    25441 gcggacggcg ttaactggcg aaactccatc acgtagagca gcgcgccggg aacggcaagc
    25501 tgttgtaatg tcgcaaggag cccgggatct tccggcagca gacgatgcag ggcgttatta
    25561 agccagataa tgtccgccga gtgagcgtgc gccgccagcg tgtctgcatt ccagagggac
    25621 agacgggcgc caggccaggg ggcgagcctc tgccgggcgc tcagcagcat ctcctggctc
    25681 tgctcaagcc cgacatactc aatctgtccg gcgttgagct gtgctaacag cgattctgcg
    25741 gcgcggccag tgcgggttcc cacctcaagc aggcgtaccg ggcgctgtaa gcgctgtgca
    25801 agctgttggc aaattgtcgc cagttcctga atatacgggg cgctggccgg atggttaaag
    25861 gccaggcttt ccggcgacca ctgcgcgtcg ttcagtaacg acagttcgtt acgggcaccg
    25921 cgaagaattt gcgataacgt taaatcatgt ccgctgaagt gcgggtgctc atcctcgcgt
    25981 tcttcaccag cgcagacctg ataaccttca gcagcgggct tcagtcgccg ttgtgtcacc
    26041 agaaatgcta accagcgttc caccacggcc tgccagcgcg gctggatagc gagtgagttc
    26101 atcaggggga gaggatcggc gccagcggtg tgacccggct tcagttttag caggcgatgc
    26161 tgaaggaaat cggctacctg ttctgcggag atctctggcg agatggcgca acagggcaac
    26221 gttcccgcaa gcgtgtgcca cgcctgcggc agtgccgggt tccgatgatc ggtaacgcaa
    26281 aaagcctcgc cctgaggaac aacgtatgcc gccagcgttt tttctttttc gccgatcgcc
    26341 agaacggttg cttgtttcac ccccaccagc tggctgagcg cgctttcgat ttcgcccagc
    26401 tcgatgcgat atcctccgac tttgacctgc ttgtcgcgac gaccgaggaa ctcgattgtg
    26461 ccatctggcc agtagcagcc gagatcgccg gtgcgatacc agcgctcgtc cgggagcgtc
    26521 aaaaattgct gctcgctacg caggggatcg ttgaaatagc cttccgcgac cccaatgccg
    26581 ccaatccata attcacccgg cacccagtca gggcagtccc ggccctgttc atccaccacc
    26641 cggtagcgtt ggttggttag cggaaaaccg taagggatgg agcgccagta ggcggggacg
    26701 tcgtgaattt cgcaggcgtt agaccagata gacgcctcgg tggcgccgcc catcgcgata
    26761 aattgtcctt gtggccggaa ggcccgataa cgggcgggga ggtcaagccc gatccagtcg
    26821 ccggaaagca tcactgcgcg caggttttcc ggcgtggcgt cggcgaaacc ttcacaccag
    26881 gtcagcagca tatcgaacag cgccgggacg ctgttccaga gcgtgacctg atggcgctgg
    26941 atcagctcac accatgcgtg aggatcgcgc cgttgatttt ccatcaccat caccagcgcg
    27001 ccgcccgcgc gcagtacgcc aaaaatgtcg taaaccgata aatcaaaatg tagggcggag
    27061 agggccagca ccctgtcatg cgggccaacc tgatagcggg tattgatatc gcaacaggtg
    27121 ttaagcgctc cccggtgaga aatgactacc cctttcggcg taccggtaga gccggaggtg
    27181 tagataatgt aggccggttg cgtgggggcg cgtactaccg ggttggcgat cggctccgcc
    27241 tcaatggcct gctgccaggc aagggcggga atatcgtctg acccggcgct ggcgtcgtgc
    27301 tgacaaatga gcaccagccg gacgctggcg tcagcgtaga ttttctcgcg ccgtgcggca
    27361 ggctgatcca gcgaaaccgg aacgtaaacc gccccggcca gcaggacggc cagaaccgca
    27421 acaagttgtc ctgcgccttt cgacatcgtg atagccacat tatcgccggg ctgaaccccg
    27481 cactcgatta acctgcccgc gcaacggcgg gcatagtctg tcagctcatg ataattccac
    27541 tgataacgca tgtccgttac cgccagcgcc tgcggctgtt gcagagcgat acggaaaatg
    27601 ccttcatgca gcaagccttc gggaatgggg gcgccggtgg cgttgacccg ttcgcgtatc
    27661 gcgcgctggc tggcgggcat catatctgcg aacggctttt gccaggcgct ttcgtcatcg
    27721 cagagttggt taatcaactg gcagtaggcg tcgaacaatg tttcgactaa cgccggaggg
    27781 aacagcgcgt cgttgctgtc ccattgtagc cagacctcgc cgtgatgctc gaacgccaga
    27841 tgatctatcc agacctgcgg cgtttgcgag atgccccatt ccggctcgcc caacggcgat
    27901 tctgcgcggc tgctgtagag ggaacgcccc agattgctgg taaataccac cggggcgccg
    27961 tgggggtagc gctgctggcg tttgagttca cggagtaatt cgacgccgga ccagtggcga
    28021 tgctcccagt cctccgtaaa cgtgagctgg tttttacgcg ccaggttgct gacggtatcg
    28081 ccatcgcagg cggtatccag cagaagaata ttggtgaagt cggcaagcat cgcgccaacc
    28141 gccggttgca gcggctggcg gtcgaataag gtgatgttaa gcagcagacg cgtcaggccg
    28201 ccccagcgag ccagcacggc agaaaaacag gtcgccagtg ccattgtcgg cgtcacgcca
    28261 tactcgccgg cccggttgct aaaggcgtgc cagcgtgttg ccgggacaat catgcggcgt
    28321 cgggtattac ggacttcacg tagcgtggcg ggttcgcagg ccagcggcaa gacgggcgcg
    28381 gggggaagcg tcgatgcttt cgccagccag taagcgcgcg cgacgtctct cagtggttga
    28441 ttgatcttct gctggtgcag caaatacgag cggaaatcat agcgggtgtc gatagccggc
    28501 agcgattctc cggccagcag ggcgttaagc tcatcgaaga aaagcgtaaa gctggaggca
    28561 tccataatca gcaggtcaat attgacatgg aggcggtggc gattgtccgg caagagcgtc
    28621 agctgaaaat caaacgtttc gccgatttct acgcgtaaaa gacggtggct caggcgctgg
    28681 cgcagtgcgt ccagataggc ctggcggctt tcagcgtcgt tatggcgtaa atcatgaacg
    28741 gtgacgccgt tccagtaagg ttgcggtagc cagacctgct gcccgtcggg gcgaaaggcg
    28801 atatgcagca ttgggtggcg ttgcagcaag gtcgtgatgg cctgctccag ctgcgacgcc
    28861 gtcagacaat ggccttcaaa ctcctgatac aggtggcaac ccacgccgcc aagcgtctgc
    28921 cccggcatgc ggcccgtcag gtaggcgtgc tgtactggcg tcaatgggaa gggggtactt
    28981 tcggtcatgt tcggccagga tgattcgtcg ggcggcgttt cttcttccgc gttctccggc
    29041 gaccggctga gcattaactg gttccatgcc gccagcgtgg gggcggcata cagctcgcga
    29101 agggtaaggc ggtagccatt tttacgaaac cagtgtaacc atctcatcaa tcttatggaa
    29161 tccagaccgg cctggatcag gttgctctct tcatgtaact gctgcggcgt taaattcagt
    29221 tcctgtatga gccgctcgcg taattgttgg taatcagccg cgtggcggtt gtccggtaac
    29281 agcgaatcct gagatggtgc gccagaaatc attcttcctc ctgatggcac gtcttaccga
    29341 aaagcattac tcatatgaga ataataatta ttaacaattg gatgctaccc gaaacgggtt
    29401 tatttatgcc cgtttcgggt tggtaacagg cggaaagaac agaggcccgg atggggtatc
    29461 cgggcctctg tcagggagga gtttaggggg gcgcgacccc ggttacatcc cgcgtttaaa
    29521 ggtcgaagga gttacgccaa actgtttctg gaaggcggca gagaaatggc tggcgttggc
    29581 atagcccaga tcggccgcca ccgtcatgac ggaggtattg ccgttggcca ggcgtcttct
    29641 ggcttcctgc attcgttcag cctgaaacag gccatagacg ctgttgttga acagaacgcg
    29701 gaagcctcgc ttaattttga gaacgctcag accggactct ctggccagct ccgcgacgcc
    29761 cggcgcttgc gtcagatcag ccagcagcag atccttcgcc cgcagcagtt tctggcgttc
    29821 catcgggttg aagtggcagg agaggcaact ggcgtcttcg cgatgttcat ccagcaccag
    29881 actgagcatg accagacttt gcccctgcaa ccatagcgtc gacggctttt gttcatgatt
    29941 gaagccgtcg tgcatgcgca tcagcgcgtg acgcaaggcc tgagccgtta agtgggtttc
    30001 agcattacaa cgctgctgct ggcaacagca gtgggagtcg atcttattct taatgaccgc
    30061 gctaatatcc ggcacccaca gcgcgagggt ttcagggctg aaggagaggg taatgctctc
    30121 gaatcggcca caataggagg cgctgccgcg acagtctggc gtatgggtaa tgtaatggcg
    30181 attgcgcgca agctcccagt cggtgtggcg ccggagtccc tgaatcgcaa agcgcgatgt
    30241 gccttccagg atgcacgaga agttaatcct gcccagatcg tcgtgtagct gcaacgtctc
    30301 aggccgcgca aaatggcccc tccagagcaa aatatccata ccttcctgca acgaacagcg
    30361 cagcagttca cactgagcag gaatattgcc atcgtttgca ggtttcccga ccaccaactg
    30421 gtgaatagag atttcagatt gcgtttgcgg tgactccgtc atgacctggt tatctccctg
    30481 tggcttagac caatgcgggt ataaatagaa ccgaatgggg tagatgagtt attgataatg
    30541 gttattattc acattaattc tcagaacgcc accctgaatt gtcgtttttg gcgtccgtaa
    30601 aaatgcatca ggaaattcag ttactcccat gtcatctcaa tcatccaata ctgaatcgct
    30661 gtcccgattt cctctctggc aggtgattac accggttcgc aggaaggtta ttttagctat
    30721 ggcgctggcg gggctggcgg cattaaccag cctgggggcg ctcctttttc tggtgtggag
    30781 cctgcgtgac attcgcgcga cgcctgacgc tattccggcc tggccgctgg gtggcgtgat
    30841 cggctgcgtg gtcttaactt ttgttctgcg cttacaggcg ttcaacacct ctcattacgc
    30901 ggcttttcat ctggagaaca ttctgcgcag caggttagcc cgtaaagcat tgcagcttcc
    30961 gcctggcgtg ttacagcaaa tgggcagcgg gtcagtggcg aaagtgatgc tggatgacgt
    31021 gaagtcgtta catatttttg tggccgacag cacgccgctc tatgcccgcg cgatcatcat
    31081 gccgctggcg acaatcgtta tcttgttctg gctggactgg cggctggcaa tcgcgacgct
    31141 gggggtactg gcgtttggat cggttgttct cgtgctcgcc cgccagcgtt cggaagatat
    31201 ggctcagcgt tatcataagg cgcgcgagca ggttagcgcg gcggttattg agttcgtgca
    31261 ggccatgccc gtggtgagaa cgtttgatag cggcagcacc agttttttgc gctatcaacg
    31321 cgcccttgaa gagtgggtcg atgtgctcca aacctggtat cgcaaagccg gtttttcagc
    31381 gcgtttttcc ttctcgattc tgaatcctct cccgaccctg tttgtcctga tctggtcggg
    31441 atacggcctg ttgcactatg gcagtttcga ttttatcgcg tgggtggccg ttttactgct
    31501 gggcagcgga atggccgaag ccgtaatgcc aatgatgatg ctcaataacc tggtcgcgca
    31561 aacgcgttta agcattcagc gtatttatca ggttctcgcg atgccggagt tatcgctgcc
    31621 gcagtctgag cagcagccgc aagaggcgag cattaccttt gagcaggtga gctttcatta
    31681 tccgcaagcg cgtactggcg ccgcgttgca ggaggtgagc tttcatgtgc ctgccgggca
    31741 aattgtggcg ctggtcgggc caagcggcgc cggaaaaagc accgtggcgc gcttgctgct
    31801 gcgttacgcc gacccggaca aaggccatat ccgtattggc ggcgtggatc tgcgtgatat
    31861 gcagacggac accctgatga agcaactctc gtttgtgttt caggacaact tcctttttgt
    31921 cgacacgata gccaataaca ttcgtctggg cgcgccggat acgccgctgg aggcggtaat
    31981 agcggcggcc agagtggcgc aggcccatga ttttattagc gctctgccag aaggttacaa
    32041 cactcgagtc ggggaacgtg gggtatttct ctccggcggc cagcggcagc gcattactat
    32101 cgcccgggcg cttttgcagg atcgccccat cctggtgctc gatgaggcga cggcgtttgc
    32161 tgacccggaa aacgaagcgg cgcttatcaa ggcgctcgcg gcggccatgc gtggccggac
    32221 ggtcatcatg gtcgcgcatc gtctctcaat ggtgactcag gccgatgtga tattgctgtt
    32281 ttccgacgga cagctcaggg aaatggggaa ccatacgcaa ctgttggcgc agggcggcct
    32341 gtatcaacgg ctctggcaac actatcagca ggcgcagcat tgggtgccgg gtggaacaca
    32401 ggaagaggtg gtggaaaatg aaagacaata atcctgcgga taacctggcc tggcgcgtca
    32461 tctggcgcca gcttatctcc agcgttggca gtcaggccag gatgctgcga cgcagtatgc
    32521 tggcgctgtt gctggcggca ttcatgcagg ggatcgcctt tgcctgtctt tatccgatca
    32581 ttgatgcgct gttacgggga gacgcgccgc aacttcttaa ctgggctatg gccttcagcg
    32641 tcgccgcaat tgtgacgctg gtgctacgct ggtatggcct gggctttgaa taccgtggtc
    32701 atctggcgca ggctacccat gagttgcgcc tgcgacttgg cgagcagtta cgccgcgtgc
    32761 cgctggagaa gctccagcgc ggcagggcgg gtgaaatgaa cgccttgctg ctgggcagcg
    32821 tggatgaaaa cctcaattat gttattgcga tagccaatat tttgctgctc accattgtca
    32881 cgccgctgac ggcgtcgctg gcgacattgt ggatagactg gcggctgggg ctggtgatgt
    32941 tgctgatctt ccctctgctg gtgccgtttt attactggcg ccgcccggcg atgcggcgac
    33001 aaatgcagac gctgggggaa gcgcaccagc gtctgagcgg cgatatcgtt gaatttgctc
    33061 aggggatgat ggtattgcgc acctgcggca gcgatgccga taaaagccgg gcgctgctgg
    33121 cgcatttcaa tgcgctggaa aacttacaga cccgcactca ccgtcagggc gctggcgcga
    33181 cgatgctgat cgccagcgtc gtggagttgg gcctacaggt ggtggtgtta tccgggatcg
    33241 tctgggtagt gacgggcact ctgaacctcg cctttttgat tgccgccgtc gcgatgatta
    33301 tgcgcttcgc agaaccgatg gcgatgttta tcagctacac ctcggttgtg gaactgatcg
    33361 ccagcgccct gcaacggatt gagcagttta tggcgatagc accgcttccc gtcgcagagc
    33421 aaagcgagat gccggaacgt tacgatatcc gctttgacaa cgtcagctat cgctacgaag
    33481 aaggcgacgg ccacgcgctt aatcatgttt ctttgacgtt cccggcagcc agtatgagcg
    33541 cgctggtggg tgcctccggc gcaggcaaaa ctaccgtcac caaactgtta atgcgctatg
    33601 ccgatccgca gcaagggcag atttctattg gcggcgtcga tattcgccgc ctgacgccgg
    33661 aacagctcaa tagcctgatt tctgttgttt tccaggatgt ctggctgttt gatgacacgc
    33721 tgctggcgaa tatccgtatc gcgtgcccac aggcgacgcg gcaggaggta gaagaggccg
    33781 cccgcgcggc gcagtgcctt gagtttattt cccgtcttcc gcaaggctgg ctgacgccaa
    33841 tgggagagat gggcggccag ctatcgggcg gcgagcgcca gcggatttcc attgccagag
    33901 cgttattgaa aaacgcgcca gtcgtcattc tcgatgaacc gactgccgcg ctggatattg
    33961 aaagcgagct ggcggtgcaa aaagcgatcg ataacctggt ttacaaccgg acggtgatta
    34021 tcatcgctca ccgtttatcc accatcgccg gggccggaaa cattctggtg atggaagagg
    34081 gacaggtggt tgagcagggt actcatgcgc aattgctctc acatcatggg cgttatcagg
    34141 cgctgtggca ggcgcaaatg gccgcgcgcg tgtggcgcga cgacggggtt tccgcgtctg
    34201 gagagtgggt gcatgagtga tgttcagtcg aatgtgaaac cgctgacgtt gacgaccggg
    34261 cgggtgattt ttgctattgc cggcgtctat gtgacgcaga gtctggtatc ggcgctgtct
    34321 atgcagtcct tacccgcgct ggtgcgcgct gctggcggtt cgctggcgct tgccggtgcg
    34381 acaaccctgt ttatgctgcc ctgggcgctg aagtttattt gggcgccgtg gatcgagcgc
    34441 tggcggcttc cgcccggtag ccaggaacgc cgttcccgca tgttaatcct gcgtggtcag
    34501 gtcgcgctag cggcgatcct gactattgcc gcagcgattg gctggtttag gcgagaaggg
    34561 ggattccccg atacgcaaat cgtcgcgtta tttgttctgt ttatggtggc aggcacggtc
    34621 gcctccacca ttgatatcgc cagcgacggc ttttgtgtcg atcaactgac ccgcacgggt
    34681 tacggctggg gaaacagcgt gcaggtcggc ggcagctatc tgggaatgat gtgcggcggc
    34741 ggggtgttca tgatgctgtc ggcggcatcc ggctggcctg tcgccatgct gatgatggcg
    34801 gtgctgatta tggcgctgtc actcccgctg tggcgcatta cggagccgac gcgaacagcg
    34861 actatcctgc atgttccggc gttaggttat gcgctaagga ggaagcaggc gcgcctgggc
    34921 ttactgctgg tattgatgct gaattcaggc atgcggtttg tgctgcctct tctggcaccg
    34981 ctgttgttgg atcatgggtt gagcatgtcc acattgggcg cgttgttcag cggcggcaat
    35041 attgcagcgg gcatagcagg aacgctggct ggcggattgc tgatgaaata cacctcaccc
    35101 ggcagagcgc tgttgacggc ttatggcgtc caggggatcg cgctgctggc ggtggtgatg
    35161 acgctcatga tggcgccggg tcatctgctg ctgcagattc tccagtgtct ggtcattgtc
    35221 cagtccattt cgctggcctg cgcgctggtc tgtctttacg ccacgctgat gtcgctttca
    35281 tcgcctttgc aggccggtgt cgacttcacc ctctttcaat gtactgatgc ggcaatcgcc
    35341 attctggcag gcgttatcgg tggcgttgtt gctcaacatt ttggctatgc ggcctgcttc
    35401 ctgtttgccg gggcattcac gttgctggcg gcgtgggttg cttatatccg gctgcattcg
    35461 gcaagagaac tgatgacaag cgcaattgat tgatccgcga ataacacaga gaggtaaggg
    35521 atgaaaatca gtgaatttct acatctggcg ttaccagagg aacaatggct accgacgatt
    35581 tctggcgttt tacgccagtt cgcagaagaa gagtgttatg tctatgagcg tcaaccctgt
    35641 tggtatttag gcaaagggtg ccaggcacgg ctgcacatta atgccgacgg aacgcaggcg
    35701 acatttattg atgatgccgg ggagcaaaaa tgggcggtgg attccattgc cgactgcgcg
    35761 cgtcgtttta tggcgcatcc tcaggtgaaa ggacgtcggg tatatggaca ggttgggttc
    35821 aactttgcgg cgcatgcgcg ggggattgcc tttaacgccg gggagtggcc gctgctgacg
    35881 ttaaccgttc cccgtgaaga acttattttt gaaaagggaa atgtcaccgt ttatgcggac
    35941 tccgccgacg ggtgccgacg tttgtgcgag tgggtaaaag aggccggtac aacgacgcag
    36001 aacgcaccac tggcggtgga taccgccctc aatggtgagg catataaaca acaggttgcg
    36061 cgcgccgttg cggagatccg ccgtggcgag tatgtcaaag tgattgtctc gcgcgccatt
    36121 cccctgccat cgcggattga tatgcccgcc acgctgttat acgggcggca ggcaaacacg
    36181 cctgtgcgct cgtttatgtt ccgtcaggaa ggacgcgaag cgctgggctt tagcccggaa
    36241 ctggtgatgt cagtgatggg caataaagtg gtcactgaac cgcttgcggg cacccgcgat
    36301 cgcatgggaa acccggagca taataaggcg aaagaggcag aactgctgca cgacagtaaa
    36361 gaggtgcttg agcatatcct ttctgtcaaa gaagctattg ctgaactgga ggctgtttgc
    36421 cagccgggca gcgtggtggt tgaagattta atgtcggttc gccagcgcgg cagcgttcag
    36481 catctggggt ctggcgtgag cggtcagctt tcggaaaaca aggatgcctg ggatgcgttt
    36541 accgtgctgt ttccgtcgat taccgcctca ggtatcccta aaaatgctgc tctgaacgcg
    36601 attatgcaaa ttgagaagac gccgcgagag ctttattccg gcgcaatcct gctgctggac
    36661 gatacgcgct ttgatgcggc gctagttctg cgttccgtat ttcaggatag ccagcgctgc
    36721 tggatacagg cgggggcggg aatcatcgcg caatctacgc cggaacgcga actgacagaa
    36781 acccgggaga aattagcgag cattgcgccc tatttaatgg tgtaggagta actgttttct
    36841 aacctcacaa ccagactgtc caaagacaat tgagtcgctc tttgattcct ctgtgactct
    36901 attgtcttct tttgggataa aataggtgtt tttcgtcatg aagttgataa aagtctatat
    36961 agaaccacct cctgtggagg tggtggtaat ttgccagagt gagggattcg ttagtatact
    37021 atgggataaa cttattttta ccaattgtat aaggcggcgc ataagattct cggcatgctt
    37081 ccagataatc cgaccaccac tgcatcatcg ctttgcgggc ttctaggtgc tctgccttat
    37141 gaatataagc catgcgcacg gtattgcgct cctgatgact catctggcgt tctactgcgt
    37201 cctttgccca taaacccgat tccatcagcg cactgcatgc cattgcccgg aagccgtgac
    37261 cgcagatatc ctttttcgtg tcgtaaccca tcacccgcag tgccttattg accgtgtttt
    37321 cacacattgg cttatacggg ttatggtcgc cggggaagat cagttcatta ttaccggtga
    37381 tatccttaat ctgtttcaga atggcgatgg actgttctga gagggggacg atatgcggca
    37441 ttcgcatttt tgccccgcgg ccggaataac gcacgccaat aatgggttct cgcgtcgcgg
    37501 gtatcgtcca gactcggttt gtgaaatcaa tctctgacca gcgggcgaaa cgcagttcac
    37561 tggagcgaat gaacacatgc agcatcagca gaacggcatg ccgggtcagt tcacggccct
    37621 gatgatatgc cccaatacgt tcaagcagtt caggcagccg ctccagcggc agggcaggat
    37681 agtgccgtct gacaggaggt gtggtcacgc cgccaaggtt tgctgcaggg ttcgtatcga
    37741 ttaactcctg atggaccgca tggcgcatta tgttactcag gtgctgccgt gtgcgggacg
    37801 caacctccag ctttgagata ccagtggcgg gagccacccg gcttgaccag cagatacaga
    37861 ccgtgagaat cggagacttt aaagggttta tcagaaggct tgagggtgcg gatttttgcg
    37921 tcggtaagga acatatgggg gtcactccat tatcgaacta acctgatccc agatttgacc
    37981 accaattttt cccgatgcat agggtaaaat caaaatgcac cgagaagagt tttcacacta
    38041 acctgttgaa tcaacatcag ataaagattt gcaaggatgc atgaaaacag aaaattggct
    38101 cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc taccgactga
    38161 actacagagg aatcgtgtga acggagcgca tattacttag cggtaccttg tctgtcaaca
    38221 ctaaatttca taagccattt caattggtta attaatctgc aaagttgtta attaatgaac
    38281 attcgtcgcc gaaaacgagt ctgtatcatt agcgtgatgc agtctctgca aaggatcttg
    38341 ttgataaaat tgacagaaac gttgccacaa tgaagggaaa cgaggagcaa aaagttctgg
    38401 ggcgccaaag aaatattcag aaagtacggc aaaacattca gcaggatcac tggcagcata
    38461 agcatcaatg ctcgctgcat tctcaccaac taattcgatt tcttcctgaa tgttgttcat
    38521 tgcagcatga agatcgtgtt cccagccagc aacctcacgc aacgaaataa agggaactcc
    38581 gctggcgcga tcgccgttac gggtgtccag cttatgagcg acttcatgaa taatcaggtt
    38641 aaaaccagat gcatcaaaag aatcttgtat atccaaccag ttcaaaacga taggcccttg
    38701 ctgccagctc tgacctgact gaacaatacg ttggttatgc accagaccga tatcgtcttc
    38761 ccattcatca tcgaccacaa atggcgcagg ataaattaag acttcatgaa aaccatccag
    38821 ccattccagt cctaactcca gaacgggtag gcaaaataga agtgctatcc ggcagcttct
    38881 taatgaatcc agttcaaagc cctgtaaagg aacaagccgc ttttgctgta aaaaacgttc
    38941 ggcaagagtg actaatttgc tttgttcctg ttccgtcaga cccgttaaaa gggggatcga
    39001 tagtgcttcc tgccagggaa gggcagtttg atgtgctgat tcttgtactt tccagggcca
    39061 cttaatcatc gttttgctcg caaactcgtc acttgaacaa aattgcacgg acagggactg
    39121 ttaaaatgcc aaatttcctg gcatcatggc aaccatctga acggagagat gccggagcgg
    39181 ctgaacggac cggtctcgaa aaccggagta ggggcaactc taccgggggt tcaaatcccc
    39241 ctctctccgc cactttatca atgacttatc tcctgacttc ccgccttgct tttcctaaac
    39301 agaacaatcg tagaatattc ttgaagggtt agatcgtcac tgttttctgt tcgatactgt
    39361 gacattcagc acttgattcg ctatggagct gacaggaagg tttcgagcga aaatctacag
    39421 ttattcagtc gttttcttat cggtcaccat tattctttta gacattgatc ctatgaaact
    39481 gccgcaaagt tgctggcggg aactgaagtt gcgtagagaa ggggtcaata attgagtcaa
    39541 aaaaggaacg cggccgtcag attaaagccg cgggacaaag tccataattt ccaagagttg
    39601 ttgaatttcc cagctttttt gaaagccttt gtatcatact aacaagtaaa aaaggtttaa
    39661 acaccaaaat taacctatat ttctggttag ctttaccatg acagatgact tgctcctgga
    39721 aacatttgcg ctgccagtct gtatggctga gttgtgtatt tattgttcgc ctttttcgca
    39781 tttttttcta cagacataat aaaaaagaca cgactcaagt atgagcgtgt ctgagtttgt
    39841 cttacttatt tgtcttggca aattattgtt gttactacca ggcactgttc tgttaaataa
    39901 ctgaagtcag cgcaaaaaaa tcaatgagac atcatctcct cgaccacttc ttcgctactc
    39961 aactgatagg gataatacgt tggccagttt tccatttcat tcaataaaga ctgttgggaa
    40021 tcattaccca taaaaatgtg aaaatgagat gattttcgtg gtgcaattat atgatcgcta
    40081 aattgtatat atttaggggc tttactttca ggatctttac attcgaataa gtaacgaacg
    40141 cctttcttgc ctgatttata ggtgagtatt ttgtatccat cgtaatcata tttacaggat
    40201 gttgtttcat tatttctatg gaattcgaca atgccgtcct caatgccaat catctcgatg
    40261 tctgtcacat aacctttgcg ataataatct ttaatttcag caaatgtttt ggttttatct
    40321 gcatccgctt tcttctgaaa gatggggtca agtttgccac tttgcagtaa aggataaacg
    40381 gattgccaga ctccatccca gtcactgagc gttcggtttt gtacattggc atcatcaaaa
    40441 acaccattag cagctttttg ttcgacctct gttaagggtt tgccgtgtga gtgatgacca
    40501 tgcgaaaagg caggagcgct aacaataaag acacctaaag caacagccag tttatgaaga
    40561 cgaatcgcca aaacagtttc ctccagagtt aatgtatatg atgtgtaatg ttatattgta
    40621 acatataaca aaatgcaaca actcttagga gacaagagaa tagttctctc aggtgtgctt
    40681 atctatctta ttgaaataaa ataaattatg tcgatgattt gaggttgatg acaaacgaaa
    40741 cataacttga tgtgatctgc gtaggccgga tagggcgctc gcgccgcatc cggcagggat
    40801 aacgcgtact ttgtcggcaa tatgaagtta tacagataat ttgttgtgaa cctgcttgcg
    40861 taaccggtta aaaagggaaa gcaacttctt ataccgtaag gctaaaagca gtaccgccag
    40921 cccagcgtag atgagtggct gcggtgaaat aatcttcact gaccacaaat aatgtattgg
    40981 ggccaggatc gcgacaagat agacgaagtt atgcaactgt tgccagtgtt taccgagttt
    41041 tcgctgcatc gcctgggttg aagtgaacgc taacgcaagc aaaattaccc agctgataat
    41101 acctaacgtt aaataaggcc gggtaattaa ctccttacct aataacgcca gattattcac
    41161 gcccagctcc agcaatgcgt aactggttaa atgcagtgtc gcccaggcaa agcaccataa
    41221 gcctaacagg cggcgagtgc gtatcaataa cggctgtttt gcgtagcgtg ccagaggtgt
    41281 gattaacaag gtcgccagca aaaatttcag tgcagtgcga ccagtaaaat gctgaatatc
    41341 tttcaccgga tcggcaccca atccaccgtg attaatcgcc cagaccagcc agagaaacgg
    41401 caacaatccg gcaagatgca ggcaaacttt cagccatgtc acctgttttg ctgtcagacg
    41461 catttagaaa ttctcccgca aatccaggcc acgatacagc gatgccacct gctcggcgta
    41521 accattaaac agtagcgttg gctggcgctg tacatcgagg atgccgcctg aaccaataaa
    41581 tcgttcggta gcctgtgacc agcgcgggtg atcaacatgc ggattaacgt tggcgtaaaa
    41641 accgtattcg tcaggcgctg ccagattcca ggtggttggc ggacgctcgc gggtcagctt
    41701 aatactgact atcgatttaa tccctttaaa gccatatttc cacggcacaa tcagtcgcac
    41761 cggcgcgcca ttttgtggcg gtaacgcctt gccataaaca cccacggtca tcagtgtgag
    41821 cggatgcatt gcttcgtcga gacgcaatcc ttcgacataa ggatatttca gcccgccacc
    41881 gataaagcgg tcctgctggc ctggcatttg ttccggtgca taaattgttt cgaaagcgac
    41941 atacttcgca ttgctggtgg gttcggcaag cgccagcaat ttgtgcagcg gaaaaccaat
    42001 ccacggcacc accatcgacc atgcttccac gcagcgcata cgataaatac gctcttccag
    42061 cggaaagcga cgggttaaat catcgtgatc gagggtcaat ggttttgcca cttcgccgct
    42121 gattttcagt gtccatggat cggttttcag gctaccagca ttagcggcgg gatcggcttt
    42181 atccagcccg aattcataga agttgttata accggagact ttatctgctg gcgtcagtgg
    42241 caggtcattt tgccaggcgg caggcttgct gaactccaac ggttttccgg cgggggcggg
    42301 tgggcgatca ttccctttga accagctaag cagatcggca tgcgcagcgt gaggcaaaga
    42361 aagtgcagcc gcgctgatgc ccagtgcttt taacacctgc cgacgcgtca taaagaatac
    42421 cgactcggcc gtaacatctg attcttttaa aaattgattc tttttcataa gccatcacac
    42481 ttttttgtct ttagccagca agagccgttt actgttaatc tgtcaggaag tgtcctccct
    42541 aacagtagca gagatctgct tttacatatt tatgcattaa ctgccacgat aggttgaata
    42601 cccatattgg ctaagtaata aaggcacatg ataatgctcg ttcactttat taatatgaaa
    42661 ttcaacgggg atctcaggga agaaactttc aagattttgt ttcttgaaat agtccccggt
    42721 tttaaatacg acacggtaat cgcccgtagt tgcagtttgc tcgggccaca gtgccttaat
    42781 tcgtccatcc ttatctgttt tggcggtatt aagttgtaac cagccgttgt ccgctttctt
    42841 ttcaagagtg actgtcacgt cggcagcggg ttttcctgtt tgctggttca aaatgtgcac
    42901 actaagaatg ttttgttgtg cagcatagac caaagaaggt aatgaaaatg cagccgttac
    42961 tacggagagt actaaataac gctttaacat gtttatatcc ttgtcatgtg aatgagtttt
    43021 gaggatagcg aaaaagcgtc cacggtaacc ttacagcagc attacaattt tgtaatgaac
    43081 caggctgttt ctataacata tgatttatgg catattattt tcatgaagat tttacttatt
    43141 gaagataatc aaaggaccca ggagtgggta acgcaggggc tttccgaagc gggttatgtc
    43201 attgatgccg tttctgatgg cagagatggg ctttatcttg cgcttaagga tgattatgca
    43261 ttgatcattc tggatattat gcttccgggt atggatggct ggcagatctt acaaacgtta
    43321 agaacagcaa agcaaactcc tgttatttgc cttactgcaa gagattctgt cgatgacaga
    43381 gtcagagggc tggacagtgg ggcaaatgat tatctggtaa aacctttttc attttctgag
    43441 ttgctggcaa gggttcgggc acaattaagg caacatcacg ctttgaattc aacattagaa
    43501 atcagtggct taagaatgga ctctgttagt caaagtgtga gcagggacaa tatcagtatt
    43561 acactgacgc gcaaggagtt tcagttactt tggctactgg cctccagagc tggcgagatt
    43621 atccccagaa cggttattgc gagtgaaatt tggggaatca actttgatag tgataccaat
    43681 acggtggacg tcgccattcg caggctccgc gcaaaagttg atgatccttt tcctgaaaag
    43741 ctaatcgcca caatccgggg gatgggctat tcattcgtag cggtaaaaaa ataatgaaaa
    43801 gattatctat aaccgtccgt ttaaccttgc tttttatatt gctgctatct gttgctggcg
    43861 ctggaattgt atggactctc tataatggcc tggcaagtga gttgaaatgg cgcgatgata
    43921 caacactcat taaccggaca gcgcagatca agcagttgtt aattgatggg gtaaatccag
    43981 atacgttacc tgtgtacttt aaccggatga tggatgttag tcaggatatc ttgatcattc
    44041 atggtgatag catcaacaaa attgttaacc ggacgaatgt cagtgatgac atgttaaata
    44101 acatacctgc tactgagaca atcagcgcag ctggcattta cagaagcatt attaatgata
    44161 cagagataga tgctttacga attaatattg atgaagtttc gccatcatta acggttactg
    44221 tggctaaatt ggcttcagcc agacataaca tgcttgaaca gtataaaatt aatagcatta
    44281 taatttgcat tgtcgccatt gtactttgct cagtattaag tccgctgtta atcagaacgg
    44341 gattacgaga gatcaaaaag ttgagtggtg taacggaagc gctgaattat aacgatagcc
    44401 gggagcctgt tgaggttagc gcattaccga gagaactaaa acctcttggg caggcgttga
    44461 ataaaatgca tcatgcttta gtcaaagatt ttgagcgtct aagtcagttt gctgacgatc
    44521 tcgctcatga acttagaacg cccattaatg cattactggg tcagaatcag gttacgctca
    44581 gtcaaaccag aagtatcgct gaatatcaaa aaacaattgc cggtaacatt gaagagctgg
    44641 aaaatatttc gcggttaaca gagaacatac tgtttcttgc ccgggcagat aaaaacaatg
    44701 ttttggtgaa actggactca ctttttctca ataaggaagt cgaaaatttg ttggattatc
    44761 ttgaatacct ttcagacgag aaagagattt gctttaaggt cgagtgcaat cagcaaatct
    44821 ttgcggataa aattttactg caacgaatgt tatcgaatct tattgttaat gccattagat
    44881 attcaccaga aaaatcgcgc attcatataa ccagttttct tgataccaac ggctatctta
    44941 atattgatat cgccagtcct ggaacgaaaa ttaatgagcc tgaaaaactc ttccgtagat
    45001 tttggcgggg agataattcg cgtcattccg aaggtcaggg acttggtctt tctttagtca
    45061 aagcgattgc cgaattgcat gggggaagtg ctacgtatca ctattttaat aagcataatg
    45121 tgttccgaat tacgttaccg caaagaaatt aacatagggc ttcagtacgc cataaatcaa
    45181 ccatgaaaaa acgcagccac gtcgtgagta ttaacgtggc tgcgattgat tatgcgctta
    45241 cattcatacg taacagggat taacccgcat aagctgccag catttcctgc gccgccagtt
    45301 tacccaacgc attcgctgcg aaaggagtgt cgccggtgag aactttacgg tccttatgta
    45361 ctcgcccggt gatgtcgtca ttaataatgt tcatgcccat tttcttcagc tcttcgccga
    45421 agtaccaggt gagatgaccc ggcatatagc ctatctctgg cgtttgtttg tctgcggcgt
    45481 ctgggaatgc gcaaatggaa taaccattca gtgggttatc gccgtggcga agcgccagaa
    45541 aagccgccgg gccgtggcaa agggagataa caaaacggtc atttttgatt gcccactgca
    45601 aagcagcagc cacgtcctgg ctttctggta aaccaataag tgcgccatga ccaccaggaa
    45661 caaagattgc tgcatattcg ctgtctgcgt tgaggctgac gacaacatcc gcgatttttt
    45721 tcggattgcg gaacaacgat ttatgctgct caaagaatgg cattactttt tcatctttgt
    45781 gcggcatagc ccagtattca aacttggtca tcagaccgga aatggtcgcc acttcgaatt
    45841 cgaaacctgc agcatggaga tgatacaacg gcagcaacgt ttcaatcgga tggttaccgg
    45901 tcgagaacag ttttccgtta tcggtcggca aataacgttc gtccgcggcg atcaccagaa
    45961 ttttatgttt accgcgatac ggttttg
//
LOCUS       DAZFJF010000020        45763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFJF010000020 DAZFJF010000000
VERSION     DAZFJF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.020"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            192..818
                     /locus_tag="ACHKAU_001316"
     CDS             192..818
                     /locus_tag="ACHKAU_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO5530417.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            839..2941
                     /locus_tag="ACHKAU_001317"
     CDS             839..2941
                     /locus_tag="ACHKAU_001317"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO5530418.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2954..3592
                     /locus_tag="ACHKAU_001318"
     CDS             2954..3592
                     /locus_tag="ACHKAU_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO5530419.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3656..4324
                     /locus_tag="ACHKAU_001319"
     CDS             3656..4324
                     /locus_tag="ACHKAU_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5530420.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4321..5106
                     /gene="phsC"
                     /locus_tag="ACHKAU_001320"
     CDS             4321..5106
                     /gene="phsC"
                     /locus_tag="ACHKAU_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO5530421.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5110..5922
                     /gene="ydhT"
                     /locus_tag="ACHKAU_001321"
     CDS             5110..5922
                     /gene="ydhT"
                     /locus_tag="ACHKAU_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO5530422.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5934..7538)
                     /locus_tag="ACHKAU_001322"
     CDS             complement(5934..7538)
                     /locus_tag="ACHKAU_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO5530423.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7664..7969)
                     /locus_tag="ACHKAU_001323"
     CDS             complement(7664..7969)
                     /locus_tag="ACHKAU_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO5530424.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8077..8153)
                     /locus_tag="ACHKAU_001324"
     tRNA            complement(8077..8153)
                     /locus_tag="ACHKAU_001324"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8117..8119),aa:Val,seq:gac)
     gene            complement(8158..8234)
                     /locus_tag="ACHKAU_001325"
     tRNA            complement(8158..8234)
                     /locus_tag="ACHKAU_001325"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8198..8200),aa:Val,seq:gac)
     gene            8542..9798
                     /locus_tag="ACHKAU_001326"
     CDS             8542..9798
                     /locus_tag="ACHKAU_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5530425.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9839..11212)
                     /gene="mdtK"
                     /locus_tag="ACHKAU_001327"
     CDS             complement(9839..11212)
                     /gene="mdtK"
                     /locus_tag="ACHKAU_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO5530426.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11427..12068
                     /gene="ribE"
                     /locus_tag="ACHKAU_001328"
     CDS             11427..12068
                     /gene="ribE"
                     /locus_tag="ACHKAU_001328"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO5530427.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12108..13256)
                     /gene="cfa"
                     /locus_tag="ACHKAU_001329"
     CDS             complement(12108..13256)
                     /gene="cfa"
                     /locus_tag="ACHKAU_001329"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO5530428.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13547..14758)
                     /gene="punC"
                     /locus_tag="ACHKAU_001330"
     CDS             complement(13547..14758)
                     /gene="punC"
                     /locus_tag="ACHKAU_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO5530429.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14871..15803
                     /gene="punR"
                     /locus_tag="ACHKAU_001331"
     CDS             14871..15803
                     /gene="punR"
                     /locus_tag="ACHKAU_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO5530430.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15800..16825)
                     /gene="purR"
                     /locus_tag="ACHKAU_001332"
     CDS             complement(15800..16825)
                     /gene="purR"
                     /locus_tag="ACHKAU_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO5530431.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17124..17213
                     /gene="cydH"
                     /locus_tag="ACHKAU_001333"
                     /gene_synonym="ynhF"
     CDS             17124..17213
                     /gene="cydH"
                     /locus_tag="ACHKAU_001333"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO5530432.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17379..18548
                     /locus_tag="ACHKAU_001334"
     CDS             17379..18548
                     /locus_tag="ACHKAU_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5530433.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18694..19275)
                     /gene="sodB"
                     /locus_tag="ACHKAU_001335"
     CDS             complement(18694..19275)
                     /gene="sodB"
                     /locus_tag="ACHKAU_001335"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO5530434.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19403..20230)
                     /gene="mepH"
                     /locus_tag="ACHKAU_001336"
     CDS             complement(19403..20230)
                     /gene="mepH"
                     /locus_tag="ACHKAU_001336"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO5530435.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20565..20912
                     /gene="grxD"
                     /locus_tag="ACHKAU_001337"
     CDS             20565..20912
                     /gene="grxD"
                     /locus_tag="ACHKAU_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO5530436.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20963..25579)
                     /locus_tag="ACHKAU_001338"
     CDS             complement(20963..25579)
                     /locus_tag="ACHKAU_001338"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO5530437.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFCEGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25672..26319)
                     /gene="rnt"
                     /locus_tag="ACHKAU_001339"
     CDS             complement(25672..26319)
                     /gene="rnt"
                     /locus_tag="ACHKAU_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO5530438.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26422..26829)
                     /gene="gloA"
                     /locus_tag="ACHKAU_001340"
     CDS             complement(26422..26829)
                     /gene="gloA"
                     /locus_tag="ACHKAU_001340"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO5530439.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26910..28007)
                     /gene="nemA"
                     /locus_tag="ACHKAU_001341"
     CDS             complement(26910..28007)
                     /gene="nemA"
                     /locus_tag="ACHKAU_001341"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO5530440.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28044..28643)
                     /gene="nemR"
                     /locus_tag="ACHKAU_001342"
     CDS             complement(28044..28643)
                     /gene="nemR"
                     /locus_tag="ACHKAU_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO5530441.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28746..28985
                     /locus_tag="ACHKAU_001343"
     CDS             28746..28985
                     /locus_tag="ACHKAU_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO5530442.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29034..29930
                     /locus_tag="ACHKAU_001344"
     CDS             29034..29930
                     /locus_tag="ACHKAU_001344"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO5530443.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30011..30532
                     /gene="sodC"
                     /locus_tag="ACHKAU_001345"
     CDS             30011..30532
                     /gene="sodC"
                     /locus_tag="ACHKAU_001345"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5530444.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30533..32545)
                     /locus_tag="ACHKAU_001346"
     CDS             complement(30533..32545)
                     /locus_tag="ACHKAU_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5530445.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32545..33402)
                     /locus_tag="ACHKAU_001347"
     CDS             complement(32545..33402)
                     /locus_tag="ACHKAU_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5530446.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33405..33641)
                     /locus_tag="ACHKAU_001348"
     CDS             complement(33405..33641)
                     /locus_tag="ACHKAU_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO5530447.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33842..34276
                     /gene="slyA"
                     /locus_tag="ACHKAU_001349"
     CDS             33842..34276
                     /gene="slyA"
                     /locus_tag="ACHKAU_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO5530448.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34323..34790)
                     /gene="slyB"
                     /locus_tag="ACHKAU_001350"
     CDS             complement(34323..34790)
                     /gene="slyB"
                     /locus_tag="ACHKAU_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO5530449.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35064..36173
                     /gene="anmK"
                     /locus_tag="ACHKAU_001351"
     CDS             35064..36173
                     /gene="anmK"
                     /locus_tag="ACHKAU_001351"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO5530450.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36271..36600
                     /gene="mliC"
                     /locus_tag="ACHKAU_001352"
     CDS             36271..36600
                     /gene="mliC"
                     /locus_tag="ACHKAU_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO5530451.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36659..37315
                     /gene="pdxH"
                     /locus_tag="ACHKAU_001353"
     CDS             36659..37315
                     /gene="pdxH"
                     /locus_tag="ACHKAU_001353"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO5530452.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37444..38718
                     /gene="tyrS"
                     /locus_tag="ACHKAU_001354"
     CDS             37444..38718
                     /gene="tyrS"
                     /locus_tag="ACHKAU_001354"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO5530453.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38777..39640
                     /gene="pdxY"
                     /locus_tag="ACHKAU_001355"
     CDS             38777..39640
                     /gene="pdxY"
                     /locus_tag="ACHKAU_001355"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO5530454.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39684..40289)
                     /gene="gstA"
                     /locus_tag="ACHKAU_001356"
     CDS             complement(39684..40289)
                     /gene="gstA"
                     /locus_tag="ACHKAU_001356"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO5530455.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40395..41897)
                     /gene="dtpA"
                     /locus_tag="ACHKAU_001357"
     CDS             complement(40395..41897)
                     /gene="dtpA"
                     /locus_tag="ACHKAU_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO5530456.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42508..43143)
                     /gene="nth"
                     /locus_tag="ACHKAU_001358"
     CDS             complement(42508..43143)
                     /gene="nth"
                     /locus_tag="ACHKAU_001358"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO5530457.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43143..43838)
                     /gene="rsxE"
                     /locus_tag="ACHKAU_001359"
     CDS             complement(43143..43838)
                     /gene="rsxE"
                     /locus_tag="ACHKAU_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO5530458.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43842..44462)
                     /gene="rsxG"
                     /locus_tag="ACHKAU_001360"
     CDS             complement(43842..44462)
                     /gene="rsxG"
                     /locus_tag="ACHKAU_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO5530459.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44466..45524)
                     /gene="rsxD"
                     /locus_tag="ACHKAU_001361"
     CDS             complement(44466..45524)
                     /gene="rsxD"
                     /locus_tag="ACHKAU_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO5530460.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45525..>45763)
                     /locus_tag="ACHKAU_001362"
     CDS             complement(45525..>45763)
                     /locus_tag="ACHKAU_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530461.1"
                     /translation="KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNA
                     EPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT        11522 a        11320 c        11931 g        10990 t
ORIGIN      
        1 tcagaataat cacattattt tttctcctaa ccataaagga ttagtttatc ggcgttaaat
       61 cttttctcat acactatcgg gttagggaaa atatctggtg ttggggacag agtcagcagc
      121 gtattagttg aatgcttaat atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg
      181 atgcgaaata aatgaacccg gttgatcgtc cactattaga tattggctta acgcgattag
      241 aattcttgcg tatatcagga aaaggccttg ctggtctaac aattgcccct gcgttactgt
      301 cacttttggg ttgcaaacag gaagatattg atagcggcac agtagggttg ataaatacac
      361 ccaaaggggt gctggtgacc cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct
      421 cttgtaccaa cttcaacgat ggctcagtag ggacattctt ctcccgtatc aaaatccacc
      481 gcaattattt ctttggcgac aacggggttg gctctggcgg cggcctgtat ggcgatctca
      541 actataccgc ggacacctgc cgtcaatgca aagagccgca atgcatgaac gtctgcccga
      601 ttggtgcgat tacctggcag cagaaagaag gctgtattac cgtcgatcat aaacgctgta
      661 ttggctgtag cgcctgtact acggcatgcc cgtggatgat ggccaccgta aataccgaaa
      721 gtaaaaaatc ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg
      781 cgttaaaaat tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat
      841 ggctaacggt tggacaggta atatattaag agtcaatctc acgacaggaa atattaccct
      901 cgaagattcc agtaagttta aaagttttgt cggtggcatg ggcttcggct acaaaattat
      961 gtatgacgaa gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc
     1021 taccggccca ttaactggat ctggtgcccc ctgtagttct cgcgtaaata tcacctcact
     1081 ttctactttt accaaaggaa atttagttgt cgatgcccat atgggtggct tttttgcagc
     1141 gcaaatgaaa ttcgctggat acgacgtcat tattatcgaa gggaaggcga aatcaccggt
     1201 atggctgaag attaaagatg acaaagttag cctggaaaaa gccgatttct tatggggaaa
     1261 agggacgcgc gcaacgacgg aagaaatttg tcgattgacc agtccggaaa cctgtgtggc
     1321 ggctattggt caggctgggg aaaaccttgt tcctctctct ggcatgttga atagccgtaa
     1381 ccacagcggc ggtgcgggaa ctggcgcaat tatgggttcg aaaaacctga aagcgattgc
     1441 ggttgaaggg acgaaagggg tcaacattgc cgatcgtcag gagatgaagc gtctcaacga
     1501 ttacatgatg actgaactta ttggtgcgaa taacaaccat gtcgtgccaa gtacgccgca
     1561 atcgtgggca gagtattcag atcccaagtc acgttggaca gcacgtaaag ggctgttttg
     1621 gggcgcggct gaaggtggtc cgattgaaac gggggaaatt ccgccaggca atcagaacac
     1681 ggtcggcttt cgtacctata aatccgtttt tgacttaggt ccggcggcag agaaatacac
     1741 agtaaaaatg agtggctgcc actcttgccc gatccgttgt atgacccaaa tgaatattcc
     1801 tcgggtgaaa gagtttggcg tgcccagcac aggtggtaac acttgtgtag ctaactttgt
     1861 ccataccacc atcttcccga acgggccgaa agattttgaa gataaagacg atggtcgtgt
     1921 gattggtaac ctggttggtc tgaatttgtt cgatgactac ggcctatggt gtaactacgg
     1981 gcagctgcat cgcgacttta cttattgtta cagcaaaggg gtgttcaagc gtgttctgcc
     2041 agctgaagag tatgcggaaa ttcactggga tcaactggaa gcgggtgacg ttaatttcat
     2101 taaagatttt tactaccgtc tggcgcatcg tgtgggtgag ctgagtcacc tggctgatgg
     2161 ttcatatgcc atcgcagaac gctggaattt gggtgaagag tactggggct acgcaaaaaa
     2221 taaactctgg tcgccgtttg gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt
     2281 cggttccatt gttaactgta tgttcaaccg tgactgcatg acgcataccc atatcaattt
     2341 tattggttcg ggtttgccat tgaaactgca gcgtgaagtg gcgaaagagc tttttggttc
     2401 tgaagatgcc tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa aaattaaata
     2461 tgcaaagtgg tcgctgttgc gggtctgttt gcataacgcc gtcaccctgt gcaactgggt
     2521 ctggccaatg accgtttcgc cgctgaaaag ccgtaattac cggggcgatc tggcgcttga
     2581 agccaaattc ttcaaagcga tcaccggcga agagatgact caggaaaaat tagatttagc
     2641 tgcagagcgt atctttacgt tgcatcgtgc ctacacggta aaactgatgc aaaccaaaga
     2701 tatgcgtaat gaacacgatc ttatctgttc ctgggtattc gacaaggatc cgcagatccc
     2761 ggtctttact gaaggtactg acaaaatgga tcgtgacgat atgcatgctt cgctcacgat
     2821 gttctacaaa gaaatgggct gggacccaca gcttggttgt ccgacccgcg aaacattgca
     2881 gcgtctgggg ctggaagata ttgccgccga tctggcagca cacaatctac tgcctgtgta
     2941 aggaatgggt gagatgaacc atcgggatga attgccgtta gcgaaagtca gcgaagttga
     3001 cgaggcgaag cggcaatggt tacaggggat gcgtcacccc gttgatacgg taaccgaacc
     3061 agaaccggct gaaatcctgg cagagttcat tcgccagcat tctgcggctg ggcagctggt
     3121 ggcgcgcgcc gtgtttctct cgccacccta ttcggttgca gaagaggagc tgtcagtttt
     3181 gctggaaagc attaagcaga acggagacta cgcggatatc gcttgtatga cgggaagcca
     3241 ggatgattac tattactcaa cgcaggccat gagtgaaaac tacgcggcga tgtctttgca
     3301 agttgtggag caggacattt gtcgcgccat tgcacacgcc gttcgttttg agtgccagac
     3361 atatcctcgt ccttacaaag tcgccatgct gatgcaagcg ccatattact tccaggaagc
     3421 gcagattgaa gccgcgatcg ccgcgatgga tgttgcacct gaatatgccg atatacggca
     3481 ggtggagtca tcgacggcgg tgttgtactt gtttagcgag cgatttatga cctatggcaa
     3541 ggcttatggc ttatgcgaat ggttcgaggt agagcagttt cagaacccct gaaatttcag
     3601 ggagatgtca tggatagggt ggacagttgc agccactgca ttaggggata atcagatgtc
     3661 cttcactcga cgcaaatttg ttctggggat gggaacagta atctttttta ctggttctgc
     3721 gtcttctctg ttagcgaaca cgaggcaaga aaaggaagtt cggtatgcca tgattcatga
     3781 cgagtcacgg tgcaatggct gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgt
     3841 ccccgctcag ggaagccgtt tatcgatagc acacattcca gtcaccgata acgacaacga
     3901 aacgcagtat cacttttttc gccagtcatg tcagcactgc gaagatgctc cgtgcatcga
     3961 cgtatgcccg acaggggcgt cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc
     4021 gcagtgcatc ggctgcagtt attgcattgg cgcttgtccg taccaggttc gctaccttaa
     4081 ccctgtcact aaagtggcag ataaatgtga tttctgcgct gagtcccgac tggcaaaagg
     4141 ctttccgccc atttgcgtca gcgcctgccc ggaacacgca ttgatctttg gtcgcgaaga
     4201 tagcccagaa attcaggcgt ggttgcagca aaataaatac tatcaatatc agctacctgg
     4261 tgccgggaaa ccgcatctgt atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt
     4321 atgaacccgt cgcaacatgc tgaacagttt cagagccagt tagcgaacta tgtgccacag
     4381 ttcactcccg aattttggcc ggtgtggttg atcattgccg gagtattgct ggttgggatg
     4441 tggttggtgc tggggctgca tgccttgctt cgtgctcgtg gcgtgaagaa atcagtcacc
     4501 gattatggtg agaagattta tctttactgc aaagcggtca gattatggca ctggtcgaat
     4561 gcgttactct ttgtattgtt gctggcaagt gggctgataa atcactttgc gctggtgggc
     4621 gcaactgcgg ttaaaagtct ggttgcggtg catgaagttt gcggattttt gttactcgca
     4681 tgctggctcg gctttgtgct gatcaatgcc gttgggggta atggtcacca ctatcgcatt
     4741 cgtcgtcagg ggtggctgga gcgagcggca aaacaaacgc gattttattt gtttggcatt
     4801 atgcaggggg aagaacatcc tttcccggca acaacccagt ctaaatttaa tcccttacag
     4861 caggtcgcct atgttggtgt tatgtatgga ttgctgccgt tgttactatt gacggggctg
     4921 ctgtgtctct atccgcaagc cgtgggagat gtgtttcctg gcgtaagata ctggttattg
     4981 caggcgcatt ttgctctggc atttataagc ctctttttta tcttcggtca tctttatctt
     5041 tgcaccacgg ggcgtacgcc acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg
     5101 cactaacgaa tgatcattac acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg
     5161 tatcgctggt tcctgcgcca tttcccacgc ggcggtagtt atgccgatat tcatcatgca
     5221 ctgattgagg aaggatatac ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa
     5281 tggctggcgg atgaaaactt cgcgcatcag gaagtttcct ctatgcaaaa actggccaca
     5341 gaccccggag agataccatt ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc
     5401 ggctgttgtg aggataacgc ccgaatcgca accgctgggt atgccgcgca aattgccagt
     5461 atgggttata gcgtgcggat tggaagtgtc ggctttaaca gtcacattgg cagctccggt
     5521 gaacgcgcac gggttgctgt aaccggtaat tcttcgcgga taagcagcgc tggagatagt
     5581 agtcggattg cgaataccgg aatgcgagta cgcgtttgta cattgggtga acggtgtcat
     5641 gtcgccagta atggtgacct ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc
     5701 aacagcggcg acaatgtaca catcattgcc agcggtgaaa attcaacggt agtgagcacg
     5761 ggcgtggtcg actcaattat tctcgggcca gggggaagcg ccgcactggc atatcatgac
     5821 ggcgaaagag ttcgcttcgc cgtcgccatt gaaggtgaaa acaatattcg aaccggagtc
     5881 cgctatcgtc ttaatgaaca gcatcagttt gttgagtgct gatagcttta cctttaatca
     5941 aacgaaagac gccgacgagc acgggatgca ggcttcacga ccgcccgagc catcgcctca
     6001 ccaatttctg cacatgccgt aagtccctga acgaaaggct ggtcgtgcat caaccagggc
     6061 aacgcgccga acgctacgcg tccacgaata tcttcgggtt gctgtaacgt ataatcttcg
     6121 ccaatatcag ggatttcatc ccctgttttc tgtaattgct cgcgtagccc agggaaagga
     6181 atatctttca ctttaagcgg acgctgtccg cgggcatcaa taaaaacgtc aaacgagtag
     6241 ctgttgtctt ccgttttcag gacggtgcgc gactcattaa tttccatttt gtagtcttca
     6301 ccgagggcga gaatatgaat gattcccgct tcacgtaagg caagtaggcg acgaatagac
     6361 tctgaaggga ttgccgcata attatcgatg aaaaccctgg caaggccttt actgaaccgt
     6421 ttatggtcgt gttcattcag atgaggaaca atttcctgta cggcttcatg caggcgcaga
     6481 atgacataac gccagggaac agtatgtttt tctcgtttat tgcgttcgac ttcctggaga
     6541 tttttttctg cccagtcaaa tgggtcgcgt tgtttgcgct cggcaaacca ggcttgagca
     6601 aaggaatcga cattcaggct ctctaaggct atgcgttggc tccagtctgg atcagcaaac
     6661 ttgatttcct ctactatcaa tctaaatacc cgatccaaaa ggccctcttc acctttttga
     6721 atctcagcat ttaatgcctg atcggtgacg atgtgtaagg gctcgtaggg aatagggcaa
     6781 tagaaatcgg cttcgggtaa aatacccgtg cgcgacatca acgtaatatt tagcttttca
     6841 cttgcgttat cgcggttaaa aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc
     6901 tgaatagcca ctgccattgc cgcatccagt ccgctcaagg atgttcccat aatacccacg
     6961 ttacacgcat cgacctttgc ttccatcagg cctgaccacg gactgggaaa atacgttctg
     7021 gttgcttctt cttcatcagg ccagacgtga cccgtggcaa tcaccgctaa atcaaacgtc
     7081 tcgctgggta aatcctgatt tgtagcgagc atgacgccag catttgtaat ttgcagatcg
     7141 gtaacctggc atgattcata aacagccact gcaaattttt gctgtcgtgc ctggtctact
     7201 aatcgtaaaa attgatcgcg gaaatattcg cccagcagaa ttcgcggtaa aaactgacga
     7261 tcgtgcaagg tttctttttt aacgccataa cgctggagat ggctggcttc ttgcttttgt
     7321 agccattcga gatacgtaca attaatcggc ggtatttcaa tactggcaat atttgccagc
     7381 atcatttttg agttttcctc atcactgtat ggcatcccga caccggcctc gtcagcctgc
     7441 tcgaagatag aaatagaaag tggagtttgt tgctgtagaa gcgagaataa ggtgtagatc
     7501 cccgtaggcc cggcacccac aatagcaatt tttttcattg ttacttacct gtacgcattc
     7561 tttcgaattt agtgttatca gataagtttg gttgagattt ttagaatttg agaaatttca
     7621 gacaggtatg aaaagggcgc taaccgccct tgattaaatt ctgtcaggcg agttttgcct
     7681 gattgatttg actcagtggc tcattgacat cgaaaacttt ggcgacaact tcctcaacgc
     7741 cgagattttt aagtcgggca gtatgttttt ccagataggc aagggcgctt ttttcatcag
     7801 taaaaagata gatcccaccg gcttcgtggt tcttttcact ttctgtccat accttccaca
     7861 gaaaaccagg ttcctgatta atcgactcag caagtggttt aagctgctca gccattgcgt
     7921 cgccaaatgg gccattaaaa gcaaaatgaa gttgtaacaa agttgccata gcaattctcc
     7981 ttctgataac gcatacgaag acagtgtaaa tcttttaacg tattggatac agagaagaaa
     8041 cagaggaaaa aaggagaaga ccagattaag aaaatctggt gcgtccgagt ggactcgaac
     8101 caccgacccc caccatgtca aggtggtgct ctaaccaact gagctacgga cgcaggatgg
     8161 tgcgttcaat tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac
     8221 tgagctatga acgcaacgtt gtaggtgaca acggggacga atattagcgg cagagtggga
     8281 aggtggcaag aggcaaaacg taattttctg cgctatttcg accgtttgca gagcttttaa
     8341 gcaaattggc tatattttgt tgatttgcaa gggcgatttt tattcaggat cgcatttaca
     8401 tctgatacaa cccaataatt cgtgtatcag agtttaaaca gagttgtttg ccaccatctc
     8461 aaatctgtcg tggataaatc ctccgtagcc tttacactta ggtagcaata aaggcacaca
     8521 gatgcagcaa aggaataaat catgggatct gatgcgaaaa acttgatgag cgacgggaat
     8581 gtgcaaattg ttaagaccgg cgaggtcatt ggcgcgacgc aacttactga aggcgagttg
     8641 attgttgaag ctggcggaag agccgaaaat accgtggtca cgggggctgg ctggttgaaa
     8701 gtggcaaccg gtgggatcgc caaatgcaca cagtacggta acaatggcac gctatcggtc
     8761 agcgacggtg ccattgccac agatattgtt cagtccgagg gaggcgcaat tagtctctct
     8821 acgctcgcta cggttaatgg ccgccatccc gaaggtgaat tcagcgttga tcagggttat
     8881 gcctgcggtt tgttgctgga aaatggcggt aacctgcgtg tactggaagg acatcgcgcg
     8941 gaaaaaatca ttctcgatca agagggcggc ctgttggtca atgggacaac ctcagctgtc
     9001 gtggtagatg aaggtggtga attgttggtg tatccaggtg gggaagccag caattgtgaa
     9061 attaatcagg gcggcgtttt tatgctggcc gggaaagcca gtgatacgtt gcttgctggt
     9121 ggcaccatga ataatctcgg tggtgaagac tctgacacta ttgttgagaa tggatccatt
     9181 tatcgtctgg ggacggatgg ccttcagctc tacagttccg gtaagacgca aaacctgtcc
     9241 gtgaatgtgg gtggtcgggc tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa
     9301 ggtggaacag tgatcctgtt gtcacccacc agcgcggacg aaaattttgt cgtagaggaa
     9361 gatcgcgcac cggttgaact gaccgggagt gttgcattac tggacggcgc ttcaatgatt
     9421 attggctatg gtgcagatct gcaacaatca acgattactg tacagcaggg cggtgtgttg
     9481 attctcgacg gcagtacggt aaaaggtgac agtgtcactt ttagtgttgg taacatcaat
     9541 ctgaatggcg gaaaactgtg gctgatcact ggtgcggcaa cgcatgtgca actgaaagtg
     9601 aaacgcctgc gcggagaggg agcgatttgc ctgcaaacca gtgcgaaaga aatctcacct
     9661 gacttcatca atgtgaaagg ggaagttacc ggggatatac gcgttgagat aacagatgcc
     9721 agtcggcaaa ctctgtgtaa cgcactgaaa ctacagccag acgaagacgg gattggcgca
     9781 acgctccagc ctgcgtaaaa acacaggcgc aacccgtgaa ggttgcgcct tgtctttatt
     9841 agcgggacgc tcgttgcaga atgatggctg acggcagacg ttgcaggaac cgcatacgca
     9901 gcatcatcat aatggctgcc gacgtcaggc caataataaa gcctatccag aagcctgctg
     9961 gccccatagg ttcaacgacc agatcggtca gtgccagaat atagccgctt ggcaagccca
    10021 gcacccagta agccgtaaag gtaatataga aaatggaacg cgtatcttta taaccacgca
    10081 aaatcccact gccaatcacc tggatcgagt cagaaatctg atataccgcc gccagcaaca
    10141 tcaaatgcgc agccagcgtt acaacctcgg gattgtcgtt gtacaacagg gcgatttgct
    10201 cccgcagtga aaccgtgaat atggccgtca gggttgccat acagacaccc accataagcc
    10261 cggtccgcgc agcggtttgc gcatccagcg ttgagccctg acccagacga taacctacgc
    10321 ggatagttac cgctgccgcc agcgacatgg gaagcacgaa cattagtgaa ctaaagttca
    10381 gggcaatctg gtgtcctgcg acatcaacaa taccgagcgg agacactaac agagccacga
    10441 cagcaaacag tgtcacttca aagaacagtg ccagcgcaat cggcaaaccg agttgaatca
    10501 gtcgtttcat aaccgcagga tcgggtttcg cggtgccttt ttcgttacga atatcgcgca
    10561 tggagcgggc gcgtttaatg taagaaacca tggcaaggaa cataacccag tacaccgccg
    10621 cagtagccac gccacaacca acgccaccga gctcaggcat accgaaatga ccataaataa
    10681 agatatagtt caccggaatg ttcaccagca ggccgataaa gcccattacc atacccggct
    10741 tggtttttgc cagaccttca cactggttac gggcaacctg gaagaacaga tagcccggcg
    10801 cgccccacaa caacgcacgc agataaccca cggctttgtc cgccagagcc ggatcgatgt
    10861 tttccatgga gcggataatg taacctgcat tccacagcac cagcataatg agaacggaaa
    10921 caaaacctgc cagccagaaa ccttgtcgca cctgatgcgc aatgcgctca cgtcgaccgg
    10981 aaccatttaa ttgcgcgata accggcgtta atgccagcag cagtccgtga ccaaagagga
    11041 tcgccggaag ccagatagaa gtaccgatag cgaccgccgc catgtcggtg gcactatagc
    11101 cgcccgccat cacggtatcg acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca
    11161 ccgggattgc taatgctaat aacagacgcg cttcactgat atacttctgc acgtgaacac
    11221 cttttatttg tagttatatg agagactaaa aagccgcctg aatgggcagc aaaaagaaga
    11281 agcaggggaa attcagtcta ttgtagcgag gatttattaa ttctccagtg aaaaaatagt
    11341 cattcttacc ccttcactgg taagggcgtt tgccacctgc tattgtgctg aacagaatgt
    11401 cttaactgat ttcaggagtt gtaagtatgt ttacggggat tgtacagggc accgcaaaac
    11461 tggtgtcgat tgacgagaaa ccaaattttc gtacgcatgt ggtggagtta cccgaccaca
    11521 tgctggacgg cctggaaacc ggtgcgtccg tggcgcataa cggttgctgc ctgaccgtga
    11581 cggaaattaa cggcaaccat gtcagttttg acctgatgaa agaaacgtta cgcattacca
    11641 atcttggcga tttaaaagtg ggggattggg taaacgttga gcgtgcggcg aaattcagtg
    11701 atgaaattgg cggacactta atgtcaggtc atattatgac cactgctgaa gtggcgaaaa
    11761 tattaacctc agaaaataat cgccagatct ggtttaaagt ccaggatagt cagttgatga
    11821 aatatattct gtacaaagga tttattggca tcgacggtat tagcctgacc gtcggcgaag
    11881 tcacgccaac gcgtttttgc gtccatttaa ttccggaaac actggaacgc acgactcttg
    11941 ggaagaaaaa actcggcgca cgcgtcaaca ttgaaattga tccacaaact caggcagtgg
    12001 tagatacggt agaacgtgtg ctggcggcac gagaaaatgc catgaatcaa ccaggtacag
    12061 aagcctgatc tgaactcgcc cccgaagagg gggcgataga atagccttta gcgagcgact
    12121 cgaaggccgt tttcaacacc gcgtgagaac accacctgcc agagctgaat atcacgggcg
    12181 cggaaagcac ctgcacaagc attcagataa taggtaaaca ttcgtttaaa gcgttcactg
    12241 tagttatctg caatttctgg ccatgcggcg aggaatcgtt cataccacgc catcaacgta
    12301 gtatcgtaat cagcaccgaa gttatgccag tcttccatca caaagtgggg ttcgctggac
    12361 tgagcaatct ggcgtacaga gggcaggcaa ccgttcggaa aaatatattt attaatccag
    12421 ggatcaacat tcagatcggt ttttttcgaa ccgatagtat ggagcaggaa tataccttcc
    12481 ggtttcaaat tacgatccac caccgcaaaa taggtatcgt aatttttcgg tccgacgtgc
    12541 tcgaacatcc ccacagaaac aatacgatca aactggtcgt tcaggtcacg ataatcttgc
    12601 agcaaaatgg tgacatccag gccttcacag cgttcctgag ccattttttg ctgttcggca
    12661 gaaatggtga cgccgaccac gcttacgtca tagttagatg ccatgtagtg cgccagtccg
    12721 ccccagccgc agccaatatc cagtacgcgc atccctggtt ttaactgcaa tttttcacaa
    12781 atcattttga gcttcgcctg ctgggcagat tccagattat cggcatcttt ccagtaagcg
    12841 caggaatatt gcatgaaggg atcaagcatg cgactgaaca agtcattacc caaatcgtaa
    12901 tgctctttgc cgactatcca ggcacgtttt ttactctgca gattgaagag acgggcgcct
    12961 gcaatacgca gcgtgtcttt gaaatgatgg gggagttggt tctcgagacc tgcgcgtaag
    13021 actttgctaa aaaacatatc cagtcggtca cattcccacc agccatccat ataactttcg
    13081 cctaacccca aagagccttc ttgcagaacg cgtttaaaaa aatcggggtt tttcacacga
    13141 atatccgccg gggcagaacc gttaatggct ataccggcac ggctaagtaa ttcgttggcg
    13201 atacggtacc agttgtcatc cggtacactg acttcttcta tacacgatga actcatagtt
    13261 tctccatcac tggtcggtga tcagaacctt aaaacagcgt agacgctttt tgggctttgt
    13321 gagaaatctc gcagaaaaaa ccgcgagtct ataatccgac gtagaacaga gggaaaggag
    13381 gaacccttgc cgaaatgacc atccatagtg agtcgggaac gatcctgtcc ccgtaaaaac
    13441 ataatattta tcgtagcttt aacaactcga aattagtata ggccgctaat ttcaatcata
    13501 caacaaaatt ttgttagcag cctaagtata agtatatcga tatagatcag tgtgattcgc
    13561 tatgagcgac ttcggcattg ccatgattct ggcagccaac ttcttcacaa cgttgcatca
    13621 tgtaacccag cgcgaccagc actactgttg ataacatcac gctggtggtg gtgagcaatg
    13681 gcgtgctgat actgatcagc caggaaacta ccagacttgc gaggaagcac agacccagtt
    13741 gcagagtgtt ctgcaacgct gcggcgcgac cggttgcatg tgggaaggga cgcagcgcct
    13801 gggcgacaac aatagggtag atcgcgccat tggcaatcgc catcacacag aatgggatca
    13861 ggatttcgac cagcgacaca tggctaatga agcccgcagc ccaggtcgca atgacgctga
    13921 cagcaaacag caccagcaac cacggtaata actgcttgcc ttgccatttc tgcagtgcgg
    13981 cgcgacagcc ataaccacca atcagaaacg cgatagtttg cgggacataa cttaaaccaa
    14041 taactgccgg gctgtagccc atttcactaa ggatgaacgg tgaaccggtc agccatgcaa
    14101 aaaaactggc tgaacaggct gcatatatca gcacgttgcc gcgataggtt ttagaacgta
    14161 gcaggtcggt aaaggtcaga ccatcctgac tattgttacg ggccttcgtc gtgggtttga
    14221 gccagaaaat aggcagaatc agcaccacgg taatggcaaa cagggtggcg aaaatcgcct
    14281 gccaggaaaa atggaccagc agccagcttc ctaacagggg agccagtgcc ggagacagac
    14341 ccaccagcgg catgatggtc gcaaaaatac ggttgacttt ctgtgaagga taataatctg
    14401 tcactaatgc ttgccagata accgccgcgg cgcagacccc cacagcctgt acaaaacgca
    14461 ataccagcag cgtagcggcg ttttctaccc acagcatccc cagactacct aacgcaaaaa
    14521 ttgtcaggcc gattaataac accggtttac gaccataacg gtcggagagt ggcccccaca
    14581 gaagctgggc tgcggcaaaa ccggcaagga acagactaag gctggcactg acagcagacg
    14641 caggcgtttg caggtcggcc tgtatggcgg cgaaagcagg cagatacata tcggttgcca
    14701 gaaaaccgag tacgctcaaa cccgccagcc agactaaaaa tcttttccca ggttgcatgt
    14761 atattctcta aatctctcaa ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt
    14821 gtgaaacggt aatatttgat gactggtttc aaaaattttg caggcagact atgtggtcag
    14881 aatattcact cgaagttgtt gatgcggtag cgcgtaatgg tagttttagc gctgcggcac
    14941 aggagctgca tcgcgttcct tctgcggtca gctataccgt gcgtcagctg gaagagtggc
    15001 tggcggtacc gctctttgaa cgacggcacc gtgatgtgga gctgaccgct gctggagcgt
    15061 ggtttctcaa agaagggcgc tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc
    15121 agcagatagc gaacggctgg cgcggtcagt tagctatcgc agtggataat attgtcaggc
    15181 cagaacgtac acggcagatg atcgttgatt tttatcgcca ttttgacgac gtcgaacttc
    15241 tggtctttca ggaagtgttt aacggtgtct gggatgcgct ttccgatggg cgcgtggaac
    15301 tggcgattgg cgcaacacgc gcgattccgg taggcggtcg ttatgccttc cgggatatgg
    15361 ggatgctaag ctggagttgc gttgttgcca gccaccaccc gctggcgttg atggatggcc
    15421 cgttcagcga tgatacgttg cgcaactggc cgtcgttggt gcgcgaagac acctcgcgaa
    15481 cgctacctaa acgtattacc tggttgctgg ataaccaaaa aagagttgtc gtgcctgact
    15541 gggaatcatc ggcaacctgt atctcggcag gattatgcat agggatggtg ccaacacatt
    15601 tcgccaaacc gtggcttaat gaggggaagt gggtggcatt agaactggaa aacccctttc
    15661 cggattcggc atgttgtctg acatggcagc aaaatgatat gtcgcctgcg ttaacctggc
    15721 tgcttgagta tctcggcgat agtgagacgc tgaataagga gtggctgcgg gagccggaag
    15781 agactcccgc aacgggtgat taacgacgat agtcgcggaa cgggccgtca gccacggagc
    15841 ggcgttcaat caagcgcgga tgcacttcaa tggactgcgg ttcttcacgt ttgttgacga
    15901 tacgatccaa cagcatgttg aacgctgttt cacccagcga atcttttggc tggtggatcg
    15961 tggtcagcgc cggcgtaaaa tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa
    16021 catcctgcgg gacgcgcaga cccatttcat cagcagcaca aagtgcgccc attgccatga
    16081 tatcgccacc acagaagacg gcagtagggc gatgcggctg cgacagtatt tgctgcatgg
    16141 cgcgataacc agattcgggt tcaaagtcac cctgaacaat ccagctttcc ggcaccttga
    16201 tcatcgcttc ttccatcgcc ttcataaaac cggcaaggcg gcctgcgccg gtgttacgtt
    16261 ccagcgggcc ggggatgacg ccgatttcgc ggtgaccgcg ttcaatcaga taacgcccgg
    16321 ccatgtagcc gccttcgaac gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt
    16381 caccccagtc catcaccacc attgggatat ggcgatactc ttccagcatc gccagcaacg
    16441 gctctgggta ctcagaacac atcaccagca gaccatcgac gcgtttttgc gccatcatcg
    16501 acagataagc ccgctgtttc tcaagattgt tccacgcatt acccagaatc agggtgtaac
    16561 ctttctggaa gcaatttttt tcaaccgctt caatgatctc ggcaaaatag gccgcttcgc
    16621 tgctggtcgc cagcaaaccg atagacttgg tgtggttaac cttcaggcta cgcgccaccg
    16681 cgctagggga gtagtgtaat tctttaatcg ctgcccacac ggcgttgcgc gtttcttcag
    16741 cgacgaaacg tgttttgttg attacgtgtg acacagttgt agtggaaacg tttgctcgtt
    16801 tcgctacatc ttttattgtt gccattccat ttcactccag accctaactt cagctcctgc
    16861 aaaatcgcaa ggtaaacgtt tgcctttaca caccttacgc acaacaaaag tgttgcggta
    16921 cgccggaaaa acgacacgga gcgtcaggaa ggggtcaatg gccagtacgc aaatagtgta
    16981 agcgtggaat tttggccgat cttgacgaaa aggggaagtg gtaaaaacac ttatcattat
    17041 aaatgacggg agattttttc atcacagtgt gtaaaaatgc gtaatatcac tatttgtgtg
    17101 gtaataaaaa aggagaaaac ttgatgagca ccgaccttaa attttcactg gtaacaacga
    17161 ttatcgtcct cggtttgatc gtggccgtgg gtttgactgc cgcgctgcac tgatttcctc
    17221 tcgagggagc gcgttctccc tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg
    17281 ttttctcttt ttgttatttt catttttttg tgattgctgt catgctttcg ccactttaat
    17341 gaatatcgcg taatgacgcg aagaggtgga gataaggcat gaaaattaac tatccgttgc
    17401 tggcgctggc gattggcgcg tttggtatcg ggacaacgga gttctctcca atgggtttgt
    17461 tgcccgtcat tgcgcgcggt gtggatgtct cgattcccgc tgccggaatg ttaatcagtg
    17521 cctatgcagt tggcgtaatg gttggcgcgc cgctgatgac gcttctactt tctcatcgtg
    17581 cccgccgcag tgcgttgatt ttcctgatgg caattttcac gctcggcaac gtactttccg
    17641 ccatcgcgcc ggattatatg accctgatgc tttcacgcat tttgaccagc ctgaatcacg
    17701 gagcattttt tggtttgggt tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg
    17761 ccagcgcagt tgccactatg tttatggggt taaccctggc aaatatcggt ggcgtgccgg
    17821 cggcgacctg gttgggtgaa accatcggct ggcggatgtc atttctggca acggcggggc
    17881 tgggagtgat ttcaatggta agtctgttct tctcattacc taaaggtggt gcaggggcac
    17941 gacctgaagt gaaaaaagag ctggcggtat taatgcgtcc gcaggtgctg tctgcattgc
    18001 tgacgacggt actgggagct ggtgcaatgt ttactctcta cacctatatc tctccggtac
    18061 tacaaagtat tacccacgca acaccagtgt tcgtcacggc aatgctggtg ctgattggtg
    18121 tcggattctc tatcggtaac tatctcggcg gcaaactggc agatcgttca gttaacggca
    18181 cgttgaaagg ctttttgttg ttgttgatgg tgattatgct ggcaatcccg ttcctggccc
    18241 gcaatgagtt cggcgcagct attagcatgg tggtgtgggg cgcagcaacc tttgcggtcg
    18301 taccgccgtt acagatgcgc gtgatgcgtg tcgccagtga agcgccaggt ctgtcttcat
    18361 cagtcaatat tggtgccttt aatcttggaa atgcgctggg agcagctgct ggtggtgcgg
    18421 taatttccgc tgggctggga tacagctttg tgccggtgat gggagcgatt gtcgcgggac
    18481 tggcattatt gctggtgttt atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta
    18541 acagctaatt aaacaaaaac gcagaaggat aaccttctgc gttacagttg ctgacaacgt
    18601 gcgatttatc ttgccgaatg cgctgttaac gcctcatccg cgttggcatg gtaagcgaag
    18661 cgcatcaggc aatgctgcat ttgccatcag ttattatgca gcgagatttt tcgctacgaa
    18721 ttcccagttc accagcgccc agaagtgctc cagatagcca ggacgtgcat tgcgatagtc
    18781 gatgtaataa gcgtgttccc agacatcaac ggtcagcagc ggagtcgcat cggtggtcag
    18841 cggagtaccc gcgttagagg ttgaaacgat agccagtttg ccatcgctgt ttttcaccag
    18901 ccaggtccag ccagaaccaa agtttttgat cgctgcatca gtaaactgcg ctttgaaatc
    18961 ggcaaagcta ccaaaagatg cggcgatagc ttcagcgact tttccagtcg gttcgccacc
    19021 ggcgttcggt gccaggcagt tccagtagaa agtatggttc cagacctgag ctgcgttgtt
    19081 gaatacgcca ccttcagagc tgcgaataat ctcttccagt gatttacctt caaacgcggt
    19141 acctttaatc aggttgttca ggttagtgac ataagtctga tgatgcttgc cgtagtgata
    19201 ctcgatagtt tccgcagaaa tgtgcggtgc cagagcatct ttagcatatg gtagtgcagg
    19261 taattcgaat gacattgcta ctctccttta ttattaattt gcatacgtac aatagcctta
    19321 ttgtgcgtat ggatagggta gcaaattaaa aggtgagaca aaagatgaac ttaccctgtt
    19381 gccgtaacaa cagggtaaag ttttagcgaa gtgtttttgg ggtcattacc cgacgagcgc
    19441 caacatagtg gcgctgccag tagtcttcac tgagagaagt gatttgaatt tcctgacctg
    19501 tgcgcggtga ctgaataaat ttgccgttgc cgacatacac gccgacatga tcggctgtgc
    19561 cgcgtccctg agtacggaaa aagaccaggt cgccgttttt cagttcacta cgttcgattg
    19621 gcgctgcatc acgcaggtga tacatttcat tcgccgtacg cggaatacga attttcacca
    19681 aatctttata agcgtaataa accaggccgc tgcaatcaaa accggtacgc ggtgagctgc
    19741 caccccaacg atatggctta ccaatttgct gcatcagttt attcattgcc actttcgtag
    19801 ctttttgcac cttcgctttg tgcgcgtcgg caagagttcc tgacgcggca tttttgactt
    19861 tcacacaatg cgatttatag cccttacgtt tggtacactt ttcggtaaag ctggctgagg
    19921 ccgttttatt gacggcgtgt ttgctgcgag ttttggcgtt tttagaagcg gtaatggaag
    19981 attttttaac ggaagaagcg gttttgcttt tggtcgtact tttactggcg gcttttttcg
    20041 ctgttttctg gctggctttt tgggtctttt tggtggttgc cgtgcttttc tttttatctg
    20101 cttttttggt gatatgagtg gtagcagaac tctccctggc ttgctttgaa gcatgggcca
    20161 taggcgttaa aggcagggtg gtaaaaagta aagcacagag cgtgatcgaa atacggttta
    20221 tccgcgccac tgagcgtccc tctgttaagg caggtctatc atgcctgcgt ctgatttaca
    20281 aaacttgatg attctaatgc atagaagaag agaaagttaa tagcttttat tgttaaaaaa
    20341 tacgtttcgt tggcgagcgc aaaaaaatgc agcagtgaaa gctaaaaatg gttataaata
    20401 cgccagtttg ccgagcatct gggaagaaac gaatatttaa aatgcaacaa atagagcaaa
    20461 gggataaaat agcaaaagcg tgatgaaaat gttattttcc gatgccgcac tgacccgcta
    20521 caatgtcaga ttactgccgt aaagaagttt gaggaagcaa gacaatgagc accactatcg
    20581 aaaaaatcca acgccagatt gctgaaaacc cgatcctgct gtacatgaaa ggttcaccga
    20641 aactgccgag ctgcggtttc tctgcccagg cagtccaggc gcttgccgca tgtggcgaac
    20701 gttttgccta tgttgatatt ctgcagaacc cggacattcg tgcggaactg cctaaatatg
    20761 ctaactggcc gaccttcccg caactgtggg ttgacggcga gctggtaggc ggttgtgata
    20821 tcgtgatcga aatgtatcag cgtggcgaac tgcagcagct gatcaaagaa actgccgcta
    20881 aatacaagtc tgaagagccg gacgcggaat aatccgtcct gcaatactga cggatgcgaa
    20941 cgggcatccg tcagtccttt ctctatcccc aatccagccc ttgtggcgaa cttgaaaatc
    21001 ccgcctcacg cagcagcgta aacatcggcg tttgccggac cggtagatca ttcacttctg
    21061 ttagcgtaaa gcgcagccgt ggttcgcgac gcagtgcggt agtcagcgcg tggaaaacct
    21121 ctggggcgag taattcctct ttttcctgcc agaccagcat ttttttgcca ccttgcgcca
    21181 gatagagtaa caatttgccg ccagaaacga ccaccagcgc acccgcccga cgcgttggaa
    21241 ccagcgttgc cggatgtgca ggccagggca gaagatttcc ccacacatta gccggatcgt
    21301 tggcagagag cgccactggt gtatagtggc gcgtttgcgt ggcttgtgtc gccagatcgc
    21361 gcaatcggtc aatagtcaga cgttcagcga attgcgcgcc acccagacct tctacaaaac
    21421 gacctcgcat aattcgcccg gagtcttcca tacttcgaca aagcgtttgc atcgatggaa
    21481 acccgccagg gatattttcg gctatcaccg cctgacgact gatgatgccg tagcggtcga
    21541 gcatattttc cgccagcgcc agcatccttt cggtatcgtt tagtggctcc acctgcaata
    21601 acgaccagcg tccagccaga tttggtgtgt tgtaagatac cagcggcgag acgggttgcg
    21661 catagacagg acgtccacga cgagcccggt gactgcggcg agttgaggtg cgtgcgttgg
    21721 aactgctgcg ggtgagggcg cgtaacggtg cccaaatgtc gctggtgatg acgccttgcc
    21781 agaccagcgc ccataacgcc tcttgcaggg cagagagatc aaccgattcg ccgattttgt
    21841 cgcgtatccg ttggctgatt tgttgtgcaa accaggctcc tccgtcagcc agaacagcga
    21901 ttatcgcttg ctgcagcgcc gaacgattcg cctgatccgc ttcggcggga gtgaacgatt
    21961 ctgcggcata ttcctgtaga tgcagtgcca ccaggccgtc atcttcaccc agcttttttt
    22021 gtcccgacca gataaccgct ccggttgcca gtaattcatc gagcatttct ggcgaatagt
    22081 cgcgtacgcg ggcaggcagg atctggcttt cccagagtga ggcgggtaaa ccgactccgg
    22141 caagctgttc gatcacccgc atcacgccat ctacgccctc ataaacgcct ggcgatgttg
    22201 aggcaaagag cgccgggcta ccatcggtgg cgggtaatac gccctgacgt tccagcagca
    22261 atcgcgcata ggtcgtggct gcaacgggac gcgtcgcttc tctggcggct tgcagcgagc
    22321 gcaaacgcag acgacgaaat acttcatcgc tcacccagat gtcttgttgc agattcatca
    22381 ccagaccctg ttcacgcagt tgctgaagct gctcttcgac aatggcaata ccgagactaa
    22441 actcatgagc cagttgttca gccgtgacca gagcatgagc ccggagatag cgcagaaaga
    22501 ggtcgcgaag cgggtaactg actctatgta aataaatctc tggcaatgac tctggtagtc
    22561 gtacgccgag ggcatcacgc agcctggcgg catcatccat acaggcaaga cgctcctgtc
    22621 cgctaatcat cgctgggaag attcgtttta ctgcaagaag attttccaga tacgacgcaa
    22681 cctcttcact gctgcctgta tgccgttgcg ccaggtcttc aacggtcatt ggccccagtt
    22741 cgcgcagcag gtcgaacaat ccttcttcac ctttcgctct tctgccagga gccagtcgtt
    22801 gcaactcttc ttccacctgg cgaatgacct gcgggtcgag taattccccc ggatcgacct
    22861 gtccgagtag attgcgcagt aactcgctgt ccagcgacag tacggatgcc cggcgctctg
    22921 ccagcggggc gtcgctctgg tacataaatt ccgcgacata gccgaacaat aaacttgtgg
    22981 cgaaaggcga gggcgtagtg gtcgttacat cggatatttg aatttcgcca ccgttcaggc
    23041 gacgcatcaa acgttccagt gcgggaagat cataaacatc ttgcagacat tcgcgtaggg
    23101 tttcgagaat gaccggaaaa tccggatatc cctgagcgat ttccagcaac tgactggcgc
    23161 gcagccgttg ttgccaaagc ggggtgcgat ggcccggagt gcgccccggc attaataatg
    23221 cccgcgcggt gcattcgcga aaacgggcgg cgaaaagtgc cgagctgcct accgcctcgc
    23281 ggacaatttg cagcaacttt tctggttcaa acaaaaaaat cgcggcgtcg ggcaatttac
    23341 catcagtgtc aggaatgcgc gcaacaatgc cgtcatcact ggcgaccaca gacgcgtcag
    23401 cgccccatag ggcatgtatt cgcccggcaa tcgccagcgc ccagggttca tgcacccgtc
    23461 ttccataggg agagtgcaaa ataatacgcc agtcgccaat ttcatcacgg caccgttcga
    23521 ggaccagatg gcgactcccc ggaacgatgc ccgtcgcgtt gcgttgctcg tcaatcaacc
    23581 cctgaatatt ggcgttcgta ttttcttctg ccagccacgg gggaatagtc ccggaaaaga
    23641 acgcgccatc cgccagtaaa tgaagaaaat cgccgatcat ctcgcctaat tcagccggac
    23701 gtccgttacc ttcaccacgc cagaagggga gccgggcaga acgacccgga gcaggagtca
    23761 caatcacctg atcgcgggtg atttgctgga tccgccatga ggtagcgccg agagtgataa
    23821 tgtcgctcac ccgcgactca tataccatct cctcatccag ttcacccacc cgccgcgaac
    23881 cggcctgttc ttcaccttcg ggtaataaca cgctatacat gccacgatcc ggaatggtgc
    23941 cgccgctggt aaccgccaac aattgagcgc caggtcgggc ggtcaatatc ccggtctccc
    24001 tgttccagac cagtttgggg cgaaaagcag aaaaatcgcc agagggatag cgcccggaaa
    24061 gcatatccag cgtggcgtca aaaacacgtc ttggcagatc tttccacggt gcggcacggc
    24121 gtacgcggga gtaccattcg tctacctgta atgcatccat cgccgcggcg gcaacggttt
    24181 gctgcgcaag gacgtcgagt ggattatgcg gcggtgtcag gttttccagc ctgcctgcga
    24241 acatacactc tacaatgact gcggaatcga ctaagtcacg ccgggtacga gggaaaaaca
    24301 gccctttaga tacaccgcca acctgatgtc cggcgcgacc aatgcgttgt aacccactgg
    24361 caacagaaag cggcgttgcc acctgaatca ccagatccac cgcgcccata tcaatcccca
    24421 gttcgagact ggaggttgcg accacgcagc gtaattcacc cgatttcagc gcctgttcgg
    24481 tgattgctcg ttgttcttta gagacggagc cgtggtgtga gcgggcaata aaaacgtcgc
    24541 tactttgtac atggttagag gttgcgccgg aggtcgactc gaaatgggcc gtatcaacgg
    24601 cgatagacgg ggaacgctgt aagcgtgcgg cgtaaagctc atttaatcgt gccgtcagtt
    24661 tttccgccag cccacgtgaa ttagtaaaga caatggtcga gcgatggcgc aacacttcat
    24721 caaggatacc cgtttcaata tatggccaga tggagccttc ccggccggca tggctgtctt
    24781 cgccggtgcc gctggcgacc gatgagacat catccatatt ggcgaccggt acgacaattc
    24841 gtatctgcgg atggcgcatt gcgggcgggt tgactaccgt aaccgggcga tcgccaccaa
    24901 gaaatgctgc cacatcgctg gctgagcgca cagtggcaga aaggccaatt cgctgtgctg
    24961 aggtgtggag cagcgcatcg agccgctcca gacttaacgc cagatgcgca ccacgtttac
    25021 tgcccgctac cgcgtggact tcatcaataa ttaccgtttc gacgccgcgt agcgtttcgc
    25081 gcgcgcggga ggtcagcatc agatagagtg attcgggtgt ggtgatcaga atatccggcg
    25141 gattacgggt gagtttgctg cattcctgtg caggcgtatc gccagtacgg atccctacgc
    25201 gaagattgac ttccgtttcg ccgcgccgcc gccgttcatc ggcaataccc ttcaacggga
    25261 gctgcaagtt gcgctgaacg tcggtgccca gagcttttat cggtgaaata tagaggatgc
    25321 gtgaggtttt acgcttatgc gcctcgcggg tatcttcgcc gccttcgcag aagagccgat
    25381 cgagggcgta gagaaatgct gccagcgttt tcccggagcc ggtcggtgca atcaccagcg
    25441 catgttcgct tcgcgccgcc acatgccagg tttgcgactg gacagcggtc ggctgtttaa
    25501 aggcgcgaag aaaccagtcg cgggtcgccg gtgaaaacac gtccggcagg agcgatgaag
    25561 ggtctggatt atctgccatt aacgctattc cccgtgaata tcatgcttga tgcgtggaat
    25621 agcctcacta tagatgcctg aatcattgca tgtcatcagg catcgactcg attacacctc
    25681 ttcggcggca ggtagcggcc agcctcccag acgtttccag cggttgacga tttcacaaaa
    25741 cagcacagca gtgcgttcgg tgtcgtacag cgcggagtgc gcctgggtgc tgtcgaagtc
    25801 catgccagcg gtctggcaag cctttgacaa tacggtttgt ccgagtgcca gcccggccag
    25861 tgcagcagtg tcaaaagtgg cgaaagggtg gaacgggtta cgtttcagtg aggcgcgttc
    25921 tgcggcggcc atcataaagc tgtgatcaaa attggcattg tgcgccacca taatggcgcg
    25981 gttacagccg ctcgctttaa tacctttacg tacaacttta aaaatttcgt gcagcgcctc
    26041 gtattcgctg accgcgccgc gatcgggatc gttcgggtca atgccgttga aggcgagggc
    26101 ctctggctgc aaatttgcgc cgacaaatgg ttcgacgtgg aaatgtaatg tggtgtccgg
    26161 catcagccag ccttgttcat ccattttcag ggtgatggcg gcaatctcaa gcagcgcatc
    26221 ggttttggcg ttaaatccgg ctgtttcaac atcgatcaca acaggataaa aaccacgaaa
    26281 acggtcgcac agaccggtaa gttgagcgtt atcggacatc tgattctctt aatacggata
    26341 aattgcagcg cgcattatga caaatattac ttgtcgatgc agtaaagatg cgggcgcgat
    26401 gagttcacgc ccggcaggag attagttgcc cagaccgcga ccggcgtctt tctcttcgat
    26461 taactcaatt ttgtaaccgt ccggatcttc cacaaacgcg ataaccgtag taccgccttt
    26521 taccggaccc gcttcacggg tcacgttacc cccgttttga cggatttttt cgcacgcttc
    26581 agcggcgtta tctacgctaa gcgcgatgtg accataagca gtgccgagtt cgtatttatc
    26641 cacgccccag ttgtaggtca gttcaatcac cgcttcttcg gtttccgggc cgtagccaac
    26701 aaacgccagt gagtatttgt attccgggtt ttcgctggta cgcagcagtt tcatgcccag
    26761 cactttggta taaaaatcga tggagcgttg cagatcgcca acgcgcagca tggtatgaag
    26821 aagacgcatc tttttatcct caaaatggct aacagattca aaatgttgtt ttagtatagc
    26881 ggcggcttta cgccgctcgc aatgttggat tacaacgtcg ggtaatcggt atagccttcc
    26941 gcgccgccac cgtagaaact ttcggcacgc tgtgggttaa gctcagcttt gcgctgcaag
    27001 cgggcgacca gatccgggtt cgcaatccag tcacgaccaa atgccaccgc atcaattaac
    27061 cctttgccga tcagcgtttc agctttttct actgtgtatg cacctgcgcc gataatcgga
    27121 ccgtggaaac gggcgcgtac tttttcgcgg aacgcatcag tatacggttc accccccgcc
    27181 caatctggtt ctgacatatg cagataagca atgccgcgtt tacccagttg ttcaatcaga
    27241 tacagtgcat cggcttcttc attcgcgccg ttatccgtgt tctggaaagt accgattggc
    27301 gaaacgcgaa tgccaatgcg atcggcaccc cattcttcaa tcccggcatc gaccacttcc
    27361 agtaccagac gtgcgcgatt ttccacgctg ccgccgtact gatcggtacg atggtttgaa
    27421 gaaggagaaa ggaactgatg cagcaaataa ccgtgagcag agtggagctc taccagatca
    27481 aaaccggctt cacgcgcgtt agcaatggcc tgacggaaat cattgacgat acctggaatc
    27541 tcttccagtt caagcgcacg cggcatggat gtttcaacac ggatcgcctg accattttca
    27601 tcgcgcagag aagtacgtgt tcccgcgcta agtgctgaag gcgctaccgg tgcctgaccg
    27661 ccaggttgca ggctggcgtg agaaatgcgt ccggtgtgcc acagctgcac ggccatatga
    27721 ccattttcag catgaacgcc agcggtgatt tttttccatg cggcaatttg ctccggacta
    27781 tggatgccag gcgcacctgc atatcctttt gcctgggcag aaatttgcgt ggcttcacta
    27841 ataatcaaac cggcactggc acgttggcga tagtattccg ccatcaacgg ggtaggaatg
    27901 tcacccggtt caatactgcg cagacgcgtc agcggtgcca taaaaatacg gtttgccgcc
    27961 gtgatcgcgc ccactttcag tggggaatac agtttttcag atgacataaa ggctcccggt
    28021 cgtctggtga taaaaaatgc ctgctaaacg gcaggcgtcg caataatgtt ttttacatgg
    28081 gccagcgcgt tttccagtgg ctcgaaactg cgcgaaattt tggcctgcag attcgcaccc
    28141 agccatagtg cgtaaagcac ttgtgcctgt tgtagcggtt cgccacaaaa ggttaaacaa
    28201 tggttctcac ggccattttc cagcgcctga gagagcaggg cgatcacgcc gcgagcacct
    28261 ttatccatcg cgctgcgcat atcttctgac agatcgcaca cttcggcaga gagttttact
    28321 gtcaggcaac cactgatggt tccatgttgg caaaactggt tcagtgtttg ctggtaataa
    28381 gccagtatgc ggtcgcggta gttaccttcg ccggattgca gcaactcagt cagtcgctgg
    28441 tgatatgcgg cgtaatgacg ctcaagcatg gcaacgccaa acgcttcttt agagcgaaag
    28501 tagtgataga aggacccttt cggcacttca gcggttttta gtaattcgct tagccccatc
    28561 ccggtgaatc cacgttgcag gcaaagttgc tcgcccgtcg ccaggagatg ttcgcgagta
    28621 tcatgttcgg tgtgtttgtt catgcgttgg agtgtagtag accagtcggt ctagtgcaag
    28681 caaagttgct actttggaca ttaacagctt caataatggt aataatctta tctgaggagg
    28741 gcgaagtggc ggagcaatta gagttctttc ccgtccagag cccgtgccgg ggaatttgcc
    28801 agtctgacga acgcgggttt tgtcgcggct gttttcgtag tcgtgatgag cgttttaact
    28861 ggaataaaat gagcgatggc gaaaaacaag aggtgctgcg cctttgtcgg cagcgattaa
    28921 tgcgcaaatt acgcgcaaat aagccagcgt catcagacga accggagcaa ccatcactct
    28981 tttgaaccta atcttgcgta tactcctctc atatctgata agaggaagtg attatggttc
    29041 agcgtattac tattgcgccg caaggcccgg agttttcccg ttttgtgatg ggctactggc
    29101 gattgatgga ctggaatatg tccgcccgcc agctggtcag ttttattgaa gagcatctgg
    29161 atctcggcgt gaccaccgtg gaccatgctg atatttatgg tggctatcag tgcgaagcgg
    29221 cgtttggcga ggcactgaaa ctggcacctc acctgcgtga acggatggaa atcgtcagta
    29281 aatgcggtat cgcgacgacc gcgcgtgaag aaaacgtcat tggtcattac atcactgacc
    29341 gcgatcacat cattaagagc gccgaacagt cgctaattaa tctcgcgacc gatcatctgg
    29401 atctgctgtt aatccaccga ccagacccgt taatggatgc cgatgaagtg gcggacgcgt
    29461 tcaaacatct gcatcagagc ggcaaagtgc gtcattttgg cgtatcgaac tttacgcctg
    29521 cgcaatttgc cctgttgcaa tcacgtctgc cgtttaccct tgccactaat caggtggaaa
    29581 tatccccggt gcatcagccg ttactgctgg atggcacgct cgaccaacta caacaactgc
    29641 gtgttcgtcc gatggcgtgg tcctgccttg gtggtggtcg tctgtttaat gatgattatt
    29701 tccagcagct gcgtgatgaa ctggctgtgg tggcagagga gttaaacgcg ggctcgattg
    29761 aacaggtggt ttacgcctgg gtattacgtt taccatcgca gccgctgcca attatcggtt
    29821 caggtaaaat tgagcgcgta cgggcagctg tcgaagcaga aacactgaaa atgacccgtc
    29881 aacaatggtt tcgtatccgt aaagcggcac tggggtacga cgtaccgtaa gcacttttag
    29941 gaatagccgc cgttcaaaaa tgtgtcactg gtttacactt attcaggaat gcacaatgaa
    30001 cggaggtcct atgaaacgtt ttagtctggc tattctggcg ctggttgttg caaccggcgc
    30061 acaagctgcc agtgaaaaag tcgagatgaa cctcgtcacg tcgcaagggg tagggcagtc
    30121 aattggtagc gtcaccatta ctgaaaccga taaaggtctg gagttttcgc ccgatctgaa
    30181 agcattaccc cccggtgaac atggcttcca tattcatgcc aaaggaagct gccagccagc
    30241 caccaaagat ggcaaagcca gcgccgcgga atccgcaggc gggcatcttg atccacaaaa
    30301 taccggtaaa catgaagggc cagaaggtgc cgggcattta ggcgatctgc ctgcactggt
    30361 cgtcaataat gacggcaaag ctaccgatgc cgtcatcgcg cctcgtctga aatcactgga
    30421 tgaaatcaaa gacaaagcgc tgatggtcca cgttggcggc gataatatgt ccgatcaacc
    30481 taaaccgctg ggcggtggcg gtgaacgcta tgcctgtggt gtaattaagt aattaagagg
    30541 ccagcgtacc ttgcggtggt gcttgctcaa gttgcgaaag cgagcagtac agccgccaga
    30601 caattgccgc cagctcacgg gcggcaggtt gatgatgacg ggcaaggctg tcgcaaatcc
    30661 gctgtaattc ttcaagtgtg gccgccagtg atttttgctg aacgccacgc tcactcatca
    30721 ctccccgcaa cagtgaaata cagttatccc gtactcgcga taacggatcg gaacgcgatt
    30781 cccagtcgcg caattgccag acaacatgag aacagttcag cagcactaca ccccagcgta
    30841 ataaccaacg gcgagccagc gcatcctggc tgttactcaa ctgactgacg tgatgataag
    30901 tgagcgattc aaattcgctt tcactcagtg ttggatggcg gcttagctga tcgacaaaat
    30961 cccggcgcag cgcgcgaata tggcggcggc ttttacgagc atccgatcct ggacgcagaa
    31021 tggcgaacgc tagccaagcc agcgcaacgc caacgatttt tgccagatta tcgttaagaa
    31081 aatcagcaaa atcatacacc gggggattag tgacagcgat aaacgaaccc ataaaaacaa
    31141 tcagttgccc ccacagtgcg gcaaatttgg gcatctgcaa tttaagaagc tgcattgtcg
    31201 ccagcagtgg aaagagaaac agtaaaaatt gccacagatc gctaatctgg accatcaggc
    31261 caaatttgac cacaaagctg aatagcgaaa gtaacaccag cgtgcgcatc agcagtgaca
    31321 acgacttaaa cggtgctgcg acggcggagt agagtacgca gctaattgct gccagcgtta
    31381 atgcattggc accggcatcc cattgcgatg caatactcca tgcgccaatc atcatcaacg
    31441 tacaaaatgt acgcagcccg ctccacatag cttcggcgtt gtcggtatga cgagccagag
    31501 cggatgtccg tgcgagtctg gcatgatcat ctacaccgct ttgcagacga tgtaattcct
    31561 gactactttg cagataaagg cggcaaaaat aacgtagtcg ctgccagaag gcgacgtgcc
    31621 ggtagtcggc gacgttggtc gggcgtagcg gggcgataat acgtgcgacg gtgtaagcat
    31681 ctgtttgcga actggcgagc gccgtcagca attgttcgag aatttctcgc gtggcacctg
    31741 gcggtgaggg ccagttgagc aacatacgtc gcaggctgga gatgacactg gtcatacgta
    31801 attgctggtg gagtagcgca ttaaggcgcg cgttttgctg gcgaaaacga tagtggctcc
    31861 agaaagcctg gatacgcagc aaattcatgg tcagtatctg cccaatcacc ccctcatgtg
    31921 ctgcacgaat ggcatcgttt gtttcaggct gccagagtaa actggcatgt tccagtaatc
    31981 gggcgtgcat gtttttcaat gcggttaaaa gggctgtagc atcggaactg ctaggcagga
    32041 tcatcatcat catgccgccg cacaaaatac cgactatcac ctcgcaaacg cgcgcctgag
    32101 cgatatccca cagctggctg gcctcagtaa tattaaccat cggaaaggcg atgatggcag
    32161 ccgtgtagcc cgccagttga aatgcatacg cgacgttatt cgtgaagtgc gcacaggccc
    32221 aggtacaaaa gccaagccac gccgacatgc tcaatagaaa aaaccacggc tcattgagcg
    32281 tatgcccggc aagaagcagt gccgcaatgg ctcccagtaa actgccagcg atgcgtccga
    32341 ggcttttgct gatgacaccg ccaacggtgg gaaagctaac cactgcagcc gaggtcatcg
    32401 cccaataggg ttcatccaga ttcaaataat aggcaaccgt cagcgccaga cacatggcaa
    32461 tggtattgcg taacgcataa cgccattgcg ccagcgtggc cttgaaccag ggcaaattgc
    32521 gcaaggacca tgacgatgcg ttcattattg acctacggca atgctacagg tggtgcccga
    32581 caccagcgtg acatcgcctg gaactttatc cagcgcaaag cggacgggaa cgcgttgggc
    32641 gagacgaacc caggggacgt taggtttcac atccggaata aggcttgagt cactttcaac
    32701 gctttgatca taaatcgccc gaccgatact ggaaacgtga ccccgtaacg ttttgttatc
    32761 actatagagg gtaatttgtg ctggtgcacc ttcgcggata tggcgcaact tggtttcttc
    32821 aaaataacca atgacgtaaa acgagtggct gtcgacaagg gcaaacagcg gtttcccggt
    32881 gtcagcgtaa tcgccgatgc gggtagtgag gttagtcacc caaccactca ccggggcgcg
    32941 aatttccgtt tgcgccagtt gccattgtgt ctgtttcagc gtggcctgtg cggcattaac
    33001 actggcctgc atcgctttaa cattgaggtt agcggtatcg agttcttcgg cagaaataaa
    33061 attttgtgag agatgacgac gacgattcgc ttcgttattg gcttttgcga ggtcagattg
    33121 cgctttcgcc agctgagcct gtgcgttcag ctcggcgatc tgaaagggcg ttttatcgat
    33181 cgtgagcaga agatcccccg cgttaaccag ttggttgtct tttatattca gctcaacaat
    33241 acgtcccgac acctgtgggg tgatagaaac cttttctgcg cgtattttcc cgtcgcgtgt
    33301 ccacggagat tgcatgtaat aattccacag ccaccatccg gcaagaaccg caactatcgc
    33361 tacaatgatt gttgagaaat acttaattgt tttaattgac atagttacca cgcaatcagt
    33421 attgccagag caaggcaaac gcaaatcgca aacagcgata aatccattaa caagggatgc
    33481 cagatgtcac cggcgtagat ccagccacga agcaggcggt gtacgacaag ccagatgacg
    33541 aatccaaacg cgaaggcttt gaaaaacgga ggaaaataga cggacgcacc gaacaccaga
    33601 tcttgtaagg gcaatcctgt tgcattgagc ataaacttca caaagtagat tcctttacga
    33661 ccgaatatat tgcgttagat taataaatat tctttaagtg cgaaaaattt acgcgcaatt
    33721 tctgaaaaat atgtctgacg gtaaccaaat gcagcaatac atttgtttta gcaatacaat
    33781 tgctgcacac tattctaaaa gccgcataat atcttagcaa gctaattata aggagatgaa
    33841 attggaatcg ccactaggtt ctgatctggc acggttggtg cgcatatggc gtgctctgat
    33901 agaccatcgc ctgaaaccgc tggagttaac acaaacccat tgggttacgt tacacaatat
    33961 ccatcagtta cctccagacc agtcgcaaat tcaactggca aaagcgattg gcatcgagca
    34021 gccatcactg gtccgtactc tggaccaact ggaagaaaaa gggttaattt cgcgtcaaac
    34081 ttgtgccagc gatcgtcggg ctaaacgtat taaactgacg gaaaaggcag agccactgat
    34141 cagcgaaatg gaagctgtta ttaacaaaac ccgcgcggaa atattacatg gcatctccgc
    34201 agaggaactg gagcaactga ttacgctcat cgcaaaactt gagcataata tcattgagtt
    34261 acaggccaaa gggtgaaatg aagggggcgt gtggtcagta acctgaccac acgcaaactt
    34321 atttagcgcg gagaaacggt cacctgactg ccattgctgg ccagtacgac acgttggccc
    34381 ggagagaaac gagtgttgcc ttgtttctgt accaccatga tggtattacc atcgtcttta
    34441 cgaatttcca gctcgacacc ctgcgttttg ttcattgcac tctgtacgcc ctgaccagct
    34501 acgccacctg caacagcgcc tgctgcagta gccagagaac gcccggttcc gccaccaaca
    34561 gtattcccca ggaaaccacc aagaacagca ccgccaattg caccgataac gttggaatca
    34621 tcaccgccct gaatctgtac cggacgtacg ttaacgatgg tgccatagct gacattctgt
    34681 acttgtttcg cttcagaagc ggtataaaca tcccctgaca gggtgtcgtt attaacacaa
    34741 ccgacaagag acagacctac cattgaaaca accaatacgc gtttaatcat tgaaaaatct
    34801 cctgttcacc atgaaacgcc acgcaagcat ccctcatggt caaagtatat ggcatatgtg
    34861 aatccatggt tacatgttct accaaatatg agcaaatcat aatgaaagta ggatcaacca
    34921 attataaaca aaacattcat gatgattatt gatttgaaga taatgtttta ctaccaacgg
    34981 taaaaatcgg tcattccgca tggtattcac gaaaagttta tgtttgagtg gtgcgcaaac
    35041 gtcagattga aaaaggatga actatgaaat cgggccgctt tattggcgtt atgtcaggca
    35101 ccagccttga tggtgttgat gttgtgttgg cgacaataga tgaacaccgg gtcgcacagc
    35161 tggcaagctt gagctggccg atcccggtgt ctctgaaaca ggctgtactg gatatttgcc
    35221 agggccagca gcttacactt tcgcagtttg gacagcttga tactcaactc gggcgacttt
    35281 ttgctgatgc ggtcaatgcc ttgcttaagg aacaaaacct gcaggcgaga gatatagttg
    35341 cgatcggctg tcacggtcaa accgtctggc atgaaccgac gggcgtggaa ccacacactt
    35401 tacagattgg cgataacaat caaattgtgg cacgcaccgg aattacggtt gtcggtgatt
    35461 ttcgccgtcg cgatattgcc ttgggaggac aaggcgcacc gctggtacct gcgttccatc
    35521 atgccttgct ggctcaccca accgagcgac gaatggtgct caatattggc ggcatcgcca
    35581 atctgtcact gctcattcct gggcagccgg ttggcggcta cgataccggt cctggtaaca
    35641 tgctgatgga tgcctggatc tggcgtcagg ccggtaaacc ttacgataaa gatgccgagt
    35701 gggcacgggc gggtaaagtt attctcccac tgctgcaaaa tatgctcagc gacccatatt
    35761 tctcgcaacc tgcaccgaaa agcaccggac gcgaatactt taactacggc tggctggagc
    35821 gccatttacg ccattttccg ggtgttgatc cccgtgatgt tcaggccaca ctggcagaac
    35881 tcaccgccgt gaccatttct gaacaagttt tgttgagcgg tggctgcgaa cgattgatgg
    35941 tatgtggtgg aggtagtcgt aatccgctac tcatggcgcg tctggcggca ttactgccag
    36001 gcacagaagt caccaccacc gatgccgttg gcattagtgg cgatgacatg gaagcattgg
    36061 ctttcgcctg gcttgcctgg cggacgctgg cgggattacc aggaaatctg ccttccgtca
    36121 ctggcgcaag ccaggagacg gtactggggg ctattttccc tgctaacccg tgatatcaga
    36181 gttaactgaa tttttcttac cgcgtctgcc gttacacttg gaacgttagg gagggcgtat
    36241 tgccctccag accaggaaag tcttcgggaa atgtctatga aaaaactgtt actgatctgt
    36301 ttgccggtgc ttctcaccgg ctgtagcgcg ttcaatcaac tggttgagca catgcaaacc
    36361 gctacgctgg aataccagtg tgatgaaaaa ccgttgacgg tcaaactgaa taatccgcgc
    36421 caggaagtca gttttgttta cgataatcaa ctactgcatc tcaaacaggg catttcagcg
    36481 tctggtgcac gttacactga cggaatctat gttttctggt cgaaaggcga tgaagcgact
    36541 gtctataaac gcgaccgcat cgtattgaat aactgtcagt tacaaaatcc acagcgttga
    36601 gatttttcca ggggcggcgc acaatagcgt cacccactga caatccgtaa agaaaaccat
    36661 gtctgataac gacgaattgc agcaaatcgc gcatctgcgc cgtgaataca ccaaaggcgg
    36721 gttacgccgt cgcgatcttc ccgccgatcc attaaccctt tttgaacgtt ggctctctca
    36781 ggcgtgtgaa gccaaactgg cagaccctac cgcgatggtg gtcgctaccg tggatgaaca
    36841 tgctcagcct tatcaacgca tcgttttact caaacattac gacgaaaaag gcatggtgtt
    36901 ttacaccaac ctcggtagcc gtaaagcaca tcaaattgaa aataatccgc gcgttagcct
    36961 gctgttcccg tggcataccc ttgaacgcca ggtgatggtg atcggtaaag cagaacgact
    37021 ttcgactctc gaagtgatga aatattttca tagccgcccg cgtgatagcc agattggtgc
    37081 atgggtttcg aagcagtcca gtcgcatttc tgcccgcggt atccttgaaa gtaaattcct
    37141 ggagctgaag cagaagtttc aacagggcga agtgccattg cccagctttt ggggcggttt
    37201 tcgcgtcagc cttgaacaga ttgagttctg gcagggcggt gagcatcgcc tgcatgaccg
    37261 ctttttgtac cagcgagaaa atgatgcgtg gaagattgat cgtcttgcac cctgaaaaga
    37321 tgcaaaaatc ttgctttaat cgctggtact cctgattctg gcactttatt ctatgtctct
    37381 ttcgcatctg gcgaaaagtc gtgtaccggc aaaggtgcag tcgttatata catggagatt
    37441 ttgatggcaa gcagtaactt gattaaacaa ttgcaagagc gggggctggt agcccaggtg
    37501 acggacgagg aagcgttagc agagcgactg gcgcaaggcc cgatcgcgct ctattgcggc
    37561 ttcgatccta ccgctgacag cttgcatttg gggcatcttg ttccattgtt atgcctgaaa
    37621 cgcttccagc aggcgggcca caagccggtt gcgctggtag gcggcgcgac gggtctgatt
    37681 ggcgacccga gcttcaaagc tgccgagcgt aagctgaaca ccgaagaaac tgttcaggag
    37741 tgggtggaca aaatccgtaa gcaggttgcc ccgttcctcg atttcgactg tggagaaaac
    37801 tctgctatcg cggcgaacaa ctatgactgg ttcggcaata tgaatgtgct gaccttcctg
    37861 cgcgatattg gcaaacactt ctccgttaac cagatgatca acaaagaagc ggttaagcag
    37921 cgtctcaacc gtgaagatca ggggatttcg ttcactgagt tttcctacaa cctgttgcag
    37981 ggttatgact tcgcctgtct gaacaaacag tacggtgtgg tgctgcaaat tggtggttct
    38041 gaccagtggg gtaacatcac ttctggtatc gacctgaccc gtcgtctgca tcagaatcag
    38101 gtgtttggcc tgaccgttcc gctgatcact aaagcagatg gcaccaaatt tggtaaaact
    38161 gaaggcggcg cagtctggtt ggatccgaag aaaaccagcc cgtacaaatt ctaccagttc
    38221 tggatcaaca ctgcggatgc cgacgtttac cgcttcctga agttcttcac ctttatgagc
    38281 attgaagaga tcaacgccct ggaagaagaa gataaaaaca gcggtaaagc accgcgcgcc
    38341 cagtatgtac tggcggagca ggtgactcgt ctggttcacg gtgaagaagg tttacaggcg
    38401 gcaaaacgta ttaccgaatg cctgttcagc ggttctttga gtgcgctgag tgaagcggac
    38461 ttcgaacagc tggcgcagga cggcgtaccg atggttgaga tggaaaaggg cgcagacctg
    38521 atgcaggcgc tggtcgattc tgaactgcaa ccttcccgtg gtcaggcgcg taaaactatc
    38581 gcctccaatg ccatcaccat taacggtgaa aaacagtccg atcctgaata cttctttaaa
    38641 gaagaagatc gtctgtttgg tcgttttacc ttactgcgtc gcggcaaaaa gaattactgt
    38701 ctgatttgct ggaaataatg cattaagtgg aaagggggag tgagaaatca ctccccctgg
    38761 tttttataca gggaacatga tgaaaaatat tctcgctatc cagtctcacg ttgtttatgg
    38821 tcatgcgggt aacagtgcgg cagagtttcc tatgcgccgc ctgggcgcga acgtctggcc
    38881 gctgaacacc gttcaatttt ctaatcacac ccaatacggc aaatggactg gctgcgtgat
    38941 gccgcccagc catttaaccg aaattgtgca aggcattgcc gccattgata aattacacac
    39001 ctgtgatgcc gtgttaagtg gctatctggg atcggcggag cagggtgaac atatcctcgg
    39061 tatcgtccgt caggtgaaag ccgcgaatcc gcaggcgaaa tatttttgcg atccggtaat
    39121 gggtcatccg gaaaaaggct gtatcgttgc accgggtgtc gcagagtttc atgtgcggca
    39181 cggtttgcct gccagcgata tcattgcgcc gaatctggtt gagctggaaa tactctgtga
    39241 gcatgcggta aataacgtcg aagaagcggt tctggcagcg cgcgaactca ttgcgcaagg
    39301 gccacaaatt gtgttggtta aacacctggc gcgagctggc tacagccgtg accgttttga
    39361 aatgctgctg gtcaccgccg atgaagcctg gcatatcagc cgtccgctgg tggattttgg
    39421 tatgcgccag ccggtaggtg ttggtgatgt gacgagcggt ttactgctgg tgaaactgct
    39481 tcagggggca acgctgcagg aggcgctgga acatgtgacc gctgcagtct acgaaatcat
    39541 ggtgaccacc aaagcaatgc aggaatatga gctgcaagtg gtggctgctc aggatcgtat
    39601 tgccaaacca gaacattact tcagcgcaac aaagctctga aatatctcat ttcggcccac
    39661 aacgacctgt gggccgatgg atattacttt aagccttccg ctgacagcgc gtcttgtact
    39721 tccggacgtt cagccatacg ttgcataaat gctgcaatgt gctctaaccc ttccagattc
    39781 agtttcaccg cgtatgccca gcgcagaacc gtaaacagat aggcatcagc aattgtaaat
    39841 ctttgcccgc agatccagtg ctcatccttc agtgcctcgt tcacatattg cagcttcttc
    39901 tccagctgcg cgcgaactgt cggtttgtac tcttccggtg tatcagggcg aaacagaggt
    39961 gtgaaacctt tatgcagctc ggtggcgatg taattcagcc attcgatggt tttatagcgg
    40021 gaaatactgt ttaccggtgc cagcaactgg cggtcgggga cgctgtcggc aagatactgc
    40081 ataatcgcta cgccttccgt cagcaaagta ccgtcatcca gcagcaatgc aggcacctgc
    40141 cccttagggt taacggcaaa gtaatcgtca ccgttttcga gacgtttttt cattaaatcc
    40201 acactgacga gggtaaaatc ctttccgctc tcacgcaggg tgatatggga agcgagagag
    40261 caggcacccg gtttgtagaa caatttcatc ggtaactcct tttgtctgtg gtttaaagta
    40321 tggtagtgcg ttgctgaaca aaaaaaaagc cgctaacgaa aagttagcgg cttgtaaaag
    40381 agtttccctg aattttacgc tacagcagct ttcgccgctt tgtctgcagc gtcatcctgc
    40441 gtcatacggt gcagtttagg cgcggtcagc agcatcagta ctgcaataat ggcagtagcg
    40501 acaccaatct gcaagaatac gcgaccataa acttccagtg acatcagcgg gtcggtaacg
    40561 ttatccggca cagccatcat acccgcaaca taaccaccaa tcaggtttgc accggcagtg
    40621 gtcaggaacc agctacccat aatgaagccc atcagacgct gcggaacgag ttgtgcaacc
    40681 attgccagac ccagaccaga gatcatcagt tccccgatgc tctgcaggcc atagcttgcg
    40741 accagccagc ttacagacac gataccagcg tcagacgcga atttcgcacc cagcggcaga
    40801 atcaggaacg caccagaaca catcaccatg ccgattgcaa acttggttgg catcggcagg
    40861 gtatcgccca tcttgttata gatagcggcc agaatcggac taccgatgat gatccagaac
    40921 gggttcagtg cctgatactg ctcaggttct acggccagac ccagaatgga gtgctcaacg
    40981 ttacgaatcg caaagaagtt cagtgatgtc ggcatctggc tgtacagcac gaagaagatg
    41041 attgcttcga gcatcaggat gaaggcaacg atcattttac gacgcgcagc acctttcatc
    41101 gcgaaggctt ctttaccgaa gataaccacg ataccgaagg caacaacgcc cagcgccata
    41161 cgcgcaactt cctgattgtg cagcagccag gtggcgatag cgatcagtgc cacaacacca
    41221 ataatggtta gcagcaggtt acggtagttg ataggctcga agtctggttt tgaaccgtac
    41281 tgtttaaccc agcgttggca gaaggcgaag ttaacgatag tgatcagcag gcctacaacg
    41341 ctcaatgcaa acgcaacgct ccagccgtat ttcgcggcca gccacggcgt agcaatcata
    41401 gagaagaaag agccgatgtt gacggacatg tagtacatgg tgaatgcacc gtccagacgc
    41461 gggtcgtttt tctcatagca tgtagaaagc agagaagacg ggttagcctt aaacaggccg
    41521 ttaccgaccg caatagccgc catacccata taaacaatac cggcgtcgtg accagaccag
    41581 gcaaccagag cataaccaat cgccagcaca atagcgccga gcataattac gcgtttagta
    41641 cccagtacct tgtcacctaa ccagccgccg atagcgacca gaccataaac cagggcacta
    41701 aaggaagaga aaagggtgat tgaatccgct tcagacatac ccagttgttt aaccaggtaa
    41761 acagccataa ttccttgtag gccgtaataa ccaaaacgtt cccataactc aatcgagaag
    41821 atgagataga acgccttcgg ttgtttgaaa gcgttcaaac tgacgctttc agttggtttt
    41881 tggtttgcag tggacacttt tacctctttt tttacatccc atattaacgg gggtgctttc
    41941 tcgcgttatg tctaaaaaac atacgcgcaa ttgttatagt gggaggggaa acggcaggta
    42001 atgttcacta tcttgcactt tcagacaagt cctttgtaat aatctgttac atataactga
    42061 ggcggggaat tcaaccattc cgggaaatgt tatccagcgt taaatttttc aaaaaacgta
    42121 acggcagatt ttttcactgc actgagaggg gggtgatggc ttaacggcga tatgttctgc
    42181 tatttagcgt ttttactagt gagatagtcc agtttctgaa aaatagccag tgtaatgttt
    42241 tgtaggtcaa tatttgttgc gtgtgtaaat ttaaatgatt ttaacctgat ttttgcaatg
    42301 tttttacatt gccacagcat cgtgatcttg atcacgaatg aatagaaaat ttcgctatta
    42361 aaaaaacaat taacctgcat ttttggttat ccgcaaacgg attacctgat gataacgcta
    42421 agcaatggca tcattcacgt taaaaagatg ttttaaaaga ggataaagaa aggttatcaa
    42481 tggggtaatc ggtgttaccc cttttcttca gatgtcaact ttctctttgt attcacaaag
    42541 atcttcaata atacaagagc cacagcgggg cttgcgggca atgcaggtat aacgcccgtg
    42601 caggatcaac caatggtggc agtcgacttt aaactctgcc ggaaccactt ttagtagctt
    42661 ttcttctacc tgttcgacat ttttccccgg cgcaaattga gtacgattac aaacgcggaa
    42721 aatgtgcgtg tcgacagcaa tagtcggcca gccgaatgca gtgtttaata cgacgttggc
    42781 tgttttacga cctacgccgg gcagggcttc aagcgcagca cgatcttccg gaaccaagcc
    42841 attatgctgc tccagtaaga tacggcaggt tttgatgata ttttctgctt tgctgttata
    42901 aagcccaatc gttttgatat aggttttaac cccttcaacg cccagttcaa gcatcgctgc
    42961 aggcgtattc gccaccgggt agagtttcgc cgtcgcctta ttaacactga catcggtcgc
    43021 ctgagcggaa agcagtacgg caatcagcaa ttcaaaaggc gaactgaaat taagctcggt
    43081 ggtgggatga ggattgttct cacgcaggcg agtgaggatc tccaggcgtt ttgctttgtt
    43141 catcagacat tccctgtttc accgtttggc aatgcacgtt cggcagctgc ttcagtacgg
    43201 cgctttttca ttctttcatc aatcaggtat tttcctgcca gcatcagtcc caggccaata
    43261 aatgcacctg gaggcagcat cgccagcagg aaaggggagt cggtgtggaa aatttccacg
    43321 cgtaatactt ttgcccagct acctaacagc gcatctgcac cgtcaaacaa tgtgccattg
    43381 ccgataattt cgcgtagtga acccagcacg aacatggcgc aggttgcgcc cataccaatt
    43441 gaaaagccgt ccagtgccga aagcgccgga ccttttttgg cggcgaaggc ttcagcgcgg
    43501 cccacaacga tacagttagt gacaatcagc ggaataaaaa tccctaatga ttgatacagg
    43561 ccaaaggcgt aggcgttgat cagcatctgt acagcgctga ccaccgaggc gatgatcatc
    43621 acgtaaatgg gaatgcggat ctcggctggc gtccagtgac gcagcgtcga aatggtcagg
    43681 ttggtcagcg tcagtaccag cgtagtcgca agtcctaaac ccagagcgtt agtggcagtg
    43741 gacgtgaccg ccaacagagg acaaaggccg agcaactgga ccagcgcaga gttgtttttc
    43801 cacaacccct gaacaataac gtctttaatt tcgctcacgc tttattctcc acaggcagga
    43861 agttgagaaa gttgtgccgg taacgtctga gcgtacaatc cggcgcgttt taccgcatta
    43921 accaccgcgc ggggagtaat cgtcgcgccg gtgaactggt cgaaatcacc accatctttc
    43981 ttcaccgccc agtgcgcatc atctgcacca ctgatttttt tacccgcaaa atgggtgatc
    44041 cagtcagaaa ggcgcagttc gattttatcg ccaagccctg gcgtttcgtg gtgctctgtc
    44101 acgcgcgtgc caagtaccgt gccgttaaaa tcggctccca ccagcagctg aatcgcaccg
    44161 gaatagccat ctggcgcggt tgcttccaga acggcggcta ccggtttgtc atcctgtttg
    44221 gcgatgtaaa cccgatgctc acctttacct aactctggcg cagttaccag atagcaactc
    44281 tgtgccagcg cattgttata gcgttcggct ggcagcacct gatcaaataa tgccttttgt
    44341 tgcagactgg cctgttcagc aatcgtcgtt ttggtcatct ggttgatggc cgcagttaac
    44401 cctgttgaac ccgctgcaaa tagcgctaac gtaatgccgt gttttcggat agttttcagc
    44461 atggtttacc ctttgcgatg gccgtagacg cgcggacgcg tgtagtaatc gatcagagga
    44521 accgtgatgt tcgccagcag gacggcaaaa gccacgccgt caggatagcc gccgaaactg
    44581 cggatcaacc agactaataa gcccgccagc gcgccgaaaa taagacgacc acgattggtc
    44641 gtagaagcgg taaccgggtc agtcaaaata aagaatgcgc cgagcatggt cgcaccagac
    44701 agcagatgaa tttgcggtgc tgccagtgtt tctggtgaga acaaccagcc caacgttgcg
    44761 cataacgcca gcgttactaa gaagctgagg ggaatatgcc agcgaatcgc tttctgccat
    44821 agcaaccaca ggccgccagc cagccaggcg agatttaccc attgccaacc agcgcccgcc
    44881 agaataccgc tgtagatcgg atattgcata atctgttcaa ccgaatgacc ggcacggaca
    44941 gaggttttaa atgtatccag cggtgtcgcc tgactaatgc catcaatacc taagcgtagt
    45001 gtgttcatat caccaccact ggcggtatgt ccgctgaaaa taacctgtat ggcgtcgata
    45061 aaaccaggga tgttgaccgc aatttcatgt ggcggtaacc agctggtcat ctgcaccggg
    45121 aaggagatca gtaagaccac ataaccaatc attgccggat taaacgggtt ttgccccaga
    45181 ccgccataca actgtttagc gatgatcacc gcaaacaccg tacccagcac gaccatccac
    45241 catggcgcga gggggggaat acttaccgcc agcaataagc ctgtcagcaa tgctgagtta
    45301 tctttcaacg ttgcggctac cgactgcttg cgtagtttga gtacgagagc ttcggctaac
    45361 agagcactaa ccgatgccaa caggatctga acgagagtac cccaaccaaa aaaccacagt
    45421 tgcgctgcga ttcctggcac ggctgcgagc aacaccaaca gcataatgcg cgatgtctgg
    45481 cgctggttat gggtataagg ggagctagct attctgaata ccatttagtc ctcgtttaca
    45541 accttctgct gggcggcttt tttggcctga gcgcgggcaa tagccgcggc aactgccgct
    45601 ttgcgcggat caacctgttc ttctggttct gcgttagtct gttgctgttc cagcttgcgc
    45661 gctttggcac gggcaatagc ggcttcgacg gcggctttgc gcggatcaac ctgttcttct
    45721 ggttccgcat tagcctgttg ctgttccagt ttgcgcgctt tgg
//
LOCUS       DAZFJF010000021        44743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFJF010000021 DAZFJF010000000
VERSION     DAZFJF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.021"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(97..1956)
                     /gene="gss"
                     /locus_tag="ACHKAU_001363"
     CDS             complement(97..1956)
                     /gene="gss"
                     /locus_tag="ACHKAU_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO5530462.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2161..3027
                     /gene="yghU"
                     /locus_tag="ACHKAU_001364"
     CDS             2161..3027
                     /gene="yghU"
                     /locus_tag="ACHKAU_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO5530463.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3151..3399)
                     /gene="hybG"
                     /locus_tag="ACHKAU_001365"
     CDS             complement(3151..3399)
                     /gene="hybG"
                     /locus_tag="ACHKAU_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO5530464.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3412..3753)
                     /gene="hypA"
                     /locus_tag="ACHKAU_001366"
     CDS             complement(3412..3753)
                     /gene="hypA"
                     /locus_tag="ACHKAU_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5530465.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3746..4234)
                     /gene="hybE"
                     /locus_tag="ACHKAU_001367"
     CDS             complement(3746..4234)
                     /gene="hybE"
                     /locus_tag="ACHKAU_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO5530466.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4227..4721)
                     /gene="hybD"
                     /locus_tag="ACHKAU_001368"
     CDS             complement(4227..4721)
                     /gene="hybD"
                     /locus_tag="ACHKAU_001368"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO5530467.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4721..6424)
                     /gene="hybC"
                     /locus_tag="ACHKAU_001369"
     CDS             complement(4721..6424)
                     /gene="hybC"
                     /locus_tag="ACHKAU_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO5530468.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6421..7599)
                     /gene="hybB"
                     /locus_tag="ACHKAU_001370"
     CDS             complement(6421..7599)
                     /gene="hybB"
                     /locus_tag="ACHKAU_001370"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO5530469.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7589..8575)
                     /gene="hybA"
                     /locus_tag="ACHKAU_001371"
     CDS             complement(7589..8575)
                     /gene="hybA"
                     /locus_tag="ACHKAU_001371"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO5530470.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8578..9696)
                     /gene="hybO"
                     /locus_tag="ACHKAU_001372"
     CDS             complement(8578..9696)
                     /gene="hybO"
                     /locus_tag="ACHKAU_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO5530471.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9885..10172)
                     /gene="yghW"
                     /locus_tag="ACHKAU_001373"
     CDS             complement(9885..10172)
                     /gene="yghW"
                     /locus_tag="ACHKAU_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO5530472.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10291..11178)
                     /gene="yghX"
                     /locus_tag="ACHKAU_001374"
     CDS             complement(10291..11178)
                     /gene="yghX"
                     /locus_tag="ACHKAU_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO5530473.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11335..12375
                     /gene="gpr"
                     /locus_tag="ACHKAU_001375"
     CDS             11335..12375
                     /gene="gpr"
                     /locus_tag="ACHKAU_001375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO5530474.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12415..12909)
                     /gene="yqhA"
                     /locus_tag="ACHKAU_001376"
     CDS             complement(12415..12909)
                     /gene="yqhA"
                     /locus_tag="ACHKAU_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO5530475.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13100..13984
                     /gene="yghA"
                     /locus_tag="ACHKAU_001377"
     CDS             13100..13984
                     /gene="yghA"
                     /locus_tag="ACHKAU_001377"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5530476.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14256..14681)
                     /gene="exbD"
                     /locus_tag="ACHKAU_001378"
     CDS             complement(14256..14681)
                     /gene="exbD"
                     /locus_tag="ACHKAU_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO5530477.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14688..15422)
                     /gene="exbB"
                     /locus_tag="ACHKAU_001379"
     CDS             complement(14688..15422)
                     /gene="exbB"
                     /locus_tag="ACHKAU_001379"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5530478.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15674..16861
                     /gene="metC"
                     /locus_tag="ACHKAU_001380"
     CDS             15674..16861
                     /gene="metC"
                     /locus_tag="ACHKAU_001380"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO5530479.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17001..17660
                     /gene="yghB"
                     /locus_tag="ACHKAU_001381"
     CDS             17001..17660
                     /gene="yghB"
                     /locus_tag="ACHKAU_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO5530480.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17700..18599)
                     /gene="yqhC"
                     /locus_tag="ACHKAU_001382"
     CDS             complement(17700..18599)
                     /gene="yqhC"
                     /locus_tag="ACHKAU_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO5530481.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18793..19956
                     /gene="yqhD"
                     /locus_tag="ACHKAU_001383"
     CDS             18793..19956
                     /gene="yqhD"
                     /locus_tag="ACHKAU_001383"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO5530482.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20061..20888
                     /gene="dkgA"
                     /locus_tag="ACHKAU_001384"
     CDS             20061..20888
                     /gene="dkgA"
                     /locus_tag="ACHKAU_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO5530483.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21088..22014
                     /gene="yqhG"
                     /locus_tag="ACHKAU_001385"
     CDS             21088..22014
                     /gene="yqhG"
                     /locus_tag="ACHKAU_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO5530484.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22065..22322
                     /gene="yqhH"
                     /locus_tag="ACHKAU_001386"
     CDS             22065..22322
                     /gene="yqhH"
                     /locus_tag="ACHKAU_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO5530485.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22365..24584)
                     /gene="ygiQ"
                     /locus_tag="ACHKAU_001387"
     CDS             complement(22365..24584)
                     /gene="ygiQ"
                     /locus_tag="ACHKAU_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO5530486.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24695..26107)
                     /gene="ftsP"
                     /locus_tag="ACHKAU_001388"
     CDS             complement(24695..26107)
                     /gene="ftsP"
                     /locus_tag="ACHKAU_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO5530487.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26182..26919)
                     /gene="plsC"
                     /locus_tag="ACHKAU_001389"
                     /gene_synonym="parF"
     CDS             complement(26182..26919)
                     /gene="plsC"
                     /locus_tag="ACHKAU_001389"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO5530488.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27152..29410)
                     /gene="parC"
                     /locus_tag="ACHKAU_001390"
     CDS             complement(27152..29410)
                     /gene="parC"
                     /locus_tag="ACHKAU_001390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO5530489.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29956..30438)
                     /gene="ygiV"
                     /locus_tag="ACHKAU_001391"
     CDS             complement(29956..30438)
                     /gene="ygiV"
                     /locus_tag="ACHKAU_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO5530490.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30491..30883)
                     /gene="ygiW"
                     /locus_tag="ACHKAU_001392"
     CDS             complement(30491..30883)
                     /gene="ygiW"
                     /locus_tag="ACHKAU_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO5530491.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31035..31694
                     /gene="qseB"
                     /locus_tag="ACHKAU_001393"
     CDS             31035..31694
                     /gene="qseB"
                     /locus_tag="ACHKAU_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO5530492.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31691..33040
                     /gene="qseC"
                     /locus_tag="ACHKAU_001394"
     CDS             31691..33040
                     /gene="qseC"
                     /locus_tag="ACHKAU_001394"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO5530493.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(33086..33418)
                     /gene="ygiZ"
                     /locus_tag="ACHKAU_001395"
     CDS             complement(33086..33418)
                     /gene="ygiZ"
                     /locus_tag="ACHKAU_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO5530494.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            33737..34318
                     /gene="mdaB"
                     /locus_tag="ACHKAU_001396"
     CDS             33737..34318
                     /gene="mdaB"
                     /locus_tag="ACHKAU_001396"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO5530495.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34349..34663
                     /gene="ygiN"
                     /locus_tag="ACHKAU_001397"
     CDS             34349..34663
                     /gene="ygiN"
                     /locus_tag="ACHKAU_001397"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO5530496.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34711..36603)
                     /gene="parE"
                     /locus_tag="ACHKAU_001398"
     CDS             complement(34711..36603)
                     /gene="parE"
                     /locus_tag="ACHKAU_001398"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO5530497.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36632..37213)
                     /gene="yqiA"
                     /locus_tag="ACHKAU_001399"
     CDS             complement(36632..37213)
                     /gene="yqiA"
                     /locus_tag="ACHKAU_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO5530498.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37213..38040)
                     /gene="cpdA"
                     /locus_tag="ACHKAU_001400"
     CDS             complement(37213..38040)
                     /gene="cpdA"
                     /locus_tag="ACHKAU_001400"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO5530499.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38065..38487)
                     /gene="yqiB"
                     /locus_tag="ACHKAU_001401"
     CDS             complement(38065..38487)
                     /gene="yqiB"
                     /locus_tag="ACHKAU_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO5530500.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38488..39117)
                     /gene="nudF"
                     /locus_tag="ACHKAU_001402"
     CDS             complement(38488..39117)
                     /gene="nudF"
                     /locus_tag="ACHKAU_001402"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO5530501.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39322..40803
                     /gene="tolC"
                     /locus_tag="ACHKAU_001403"
     CDS             39322..40803
                     /gene="tolC"
                     /locus_tag="ACHKAU_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO5530502.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40951..41622
                     /gene="ygiB"
                     /locus_tag="ACHKAU_001404"
     CDS             40951..41622
                     /gene="ygiB"
                     /locus_tag="ACHKAU_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO5530503.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41628..42788
                     /gene="ygiC"
                     /locus_tag="ACHKAU_001405"
     CDS             41628..42788
                     /gene="ygiC"
                     /locus_tag="ACHKAU_001405"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5530504.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42826..43614)
                     /gene="ygiD"
                     /locus_tag="ACHKAU_001406"
     CDS             complement(42826..43614)
                     /gene="ygiD"
                     /locus_tag="ACHKAU_001406"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO5530505.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43757..44530
                     /gene="zupT"
                     /locus_tag="ACHKAU_001407"
     CDS             43757..44530
                     /gene="zupT"
                     /locus_tag="ACHKAU_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO5530506.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44588..>44743)
                     /gene="yqiD"
                     /locus_tag="ACHKAU_001408"
     CDS             complement(44588..>44743)
                     /gene="yqiD"
                     /locus_tag="ACHKAU_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO5530507.1"
                     /translation="YWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYLNSTRE
                     ETAEKVE"
BASE COUNT        11038 a        11967 c        11233 g        10505 t
ORIGIN      
        1 aatgaataaa tattggaata tataaatatt gaatattttg attaatcccc gtactaatta
       61 ttcttcataa tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg
      121 ttcaatatca ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc
      181 gtagttgccg ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa
      241 acaccacagt tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag
      301 cacctcttca tgatggctga ccaggttgat attgctacca cagcgaccgg caatcggttt
      361 cactgcgtaa cctgttttca ccaattcatc attaacagtg aaatcggtat ccagcaggta
      421 acgatggtgc gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac
      481 cgtccacagc ggctcaaaga ctagcacttc cgggcgcagc aatacgtcga taagacgcac
      541 ttcgttttgc ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc
      601 acgaatctga tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagccg
      661 tccttcccca tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa
      721 gatacgcgtt tcaaagccct cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt
      781 ttcctcgata tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca
      841 ggcaccagcc agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc
      901 ctgctccgcc caacgttcga ggatcaagcc cgcttcggta tgacatgagg cagagtcggc
      961 gttgtattcg taaaccttca ggccacgctc atccatgcag aaatccatac gaccagtgat
     1021 catatggtga cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat
     1081 gtcgaacagc gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat
     1141 caggtgcagc tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta
     1201 gtaatgataa ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgcag
     1261 cggatctttt tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc
     1321 gctgattttc agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc
     1381 cgtctggatc atccagccca gaatggtggt gtcatcaaaa gtgtctttca gggtatagca
     1441 gccgttttcg accaccatct ccagctcacg cgtccactgt tgcccttgcg gcaacgggga
     1501 atgaatcacg ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc
     1561 gacatggcca gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac
     1621 cggcgcacgc ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac
     1681 ttctcgcagg aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac
     1741 accgtaattc agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat
     1801 atattcgtcg tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag
     1861 agaactgtaa tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc
     1921 gaacggggca tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc
     1981 taagcagttg gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga
     2041 ggtcagacta atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat
     2101 tctgtacagc aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact
     2161 atgacagaca atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc
     2221 gcgttcgcca atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt
     2281 ggcaaacacc cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt
     2341 atgcttgagg agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt
     2401 cgtattggcg atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag
     2461 atcccggcgc tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg
     2521 atcctgcttt atctggcgga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt
     2581 actgaaacga tgaactggct gttctggtta cagggcgcgg caccgttcct cggcggtggt
     2641 tttggtcact tttaccatta cgcgccgatg aaaattgagt acgccatcaa ccgctttacc
     2701 atggaagcca aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttatt
     2761 gcgggcgatg agtacaccat tgcggatatg gcgatttggc cgtggtttgg caatgtggtg
     2821 ttaggcggcg tgtatgatgc cgcagagttt cttgatgcgg gcagttataa gcatgtacaa
     2881 cgctgggcga aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc
     2941 accaacggac cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg
     3001 aatacggaag ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc
     3061 cggataaggc gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctgccg
     3121 cgtcttatca gtatctacga aagctaatca tcacgcgctg gtaatgtcgt aatccatttg
     3181 gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca tggcaaatcc
     3241 gacgtgtacc agcacccact ggcccagtag atcggcaggg ttaccttcac aaatcagggc
     3301 gatattcacg tcgcgcttga taccacatac ttcgacctgc gcaagctggt gaatatcttc
     3361 accgacagcc agcacctggc ctggaacgcc aatacacatt attaactccg gttattcaac
     3421 ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca
     3481 gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat cccagcacca
     3541 agcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca
     3601 gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg cgccaatttc
     3661 cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt ggataatttc
     3721 aacggcgctc tggcaaagag acaactcatg cattttcgcc actcctgcga ccaaacagca
     3781 gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tgacagcgaa aggatcattc
     3841 gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catgctgtgc gaaagcggcg
     3901 acatcagcga acaggagaga tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca
     3961 tagtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc ggccagagtt
     4021 gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca caccccgtcc
     4081 actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga tgcagaaaag
     4141 agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg
     4201 gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg gatggcttcc
     4261 acgccagatt cacgcagcgc agccagaacc tgctcaagcg caggttcaat cattgcttca
     4321 accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc gaccagggtc
     4381 agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc caggccaagc
     4441 tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg caggatcatc
     4501 atcgttcccg gcgtgttctt tttcgacaca atggcatccg caataatcag atgatcgcga
     4561 tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat ctcaacataa
     4621 tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg
     4681 gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc ttcactgaaa
     4741 ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac gggtcgaagg
     4801 agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt gtacccacca
     4861 gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag ttccaggttg
     4921 atggaacaac tgcctggtag ttgctgatga taccgtcttt aatcaccatc cagtgagaga
     4981 gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt gccggaatgt
     5041 tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt
     5101 ggttttgcag gatatcctgc aattcacagc agtgaacggt acgaccaata atacggccca
     5161 gcgtggagtg cagctgtgcc acttccagcg tgttgccagt cagtttctga taaatcgcaa
     5221 cgatttcatt cagtttgttc tgggtagatt ctcgacctgc cgccagtttc accagcatat
     5281 tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc acccaggaat
     5341 atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc cacggcgcct
     5401 gtggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt
     5461 attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc tcaatgtagc
     5521 cgcccgggaa caggaagctg ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt
     5581 agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg
     5641 tatcaacctt ataaacctgc tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt
     5701 acatcaggcg ctcaaggttc aacacgccca acccgtcgag gttgattggg ttcgcgacac
     5761 cacccactgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt
     5821 tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc aggttcactt
     5881 ccggcggcag tttcatcgct gggtgacccc agtagccatt agcgaaaata cccaactgac
     5941 cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc ggactgttca
     6001 gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca gcttgcagtg
     6061 cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg atatggtcat
     6121 gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc ggaacgtcga
     6181 tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta gtacatacgc
     6241 cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc acgatctctt
     6301 ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca ttttcgattt
     6361 cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta attctctggc
     6421 tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag gaagtatcgg
     6481 cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac caatagaaat
     6541 caacagttct tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga atgccaccag
     6601 cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg acaggaacag
     6661 catgcgggaa tcattacgca gcttcgccac acgcagaacc accagcggga agagcatcag
     6721 caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta acgacagctt
     6781 gtcgcgatag ataagctcgc caaagcgcag cacgatgaaa atcgccagca acacactgat
     6841 ggtgttggtc agcttgacga acagactctt ttcatccgga ccgttgccac gcagacccgc
     6901 ctgcaccagc gaaccttcaa agatgacaat cgagaagccc atgatgaacg ccgtcagcag
     6961 cgagaacagc ggcaacattt catagctctg ccacaacgga tgcaccttgt agcccgccga
     7021 gatcatcagt gaccccattg aagactggtg catggtcggc aacagcgcgc caagtgcaat
     7081 gatgaagaac atcaccttgt tcagacgctt gagagaaacc ttccagccca gacgttcaaa
     7141 cagtgccgga gcaaactcca gtgccatcac gccgatatag atggtcatac agaccgccgt
     7201 ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg gcaggttcca
     7261 gtagcgaccc acgtcgatag tgatcgacaa gccccccagt gagtaaccaa acagactcgc
     7321 caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt ataccgccca
     7381 cgccagcgcc cagccgccac aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca
     7441 cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga acaccagacg
     7501 cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga ccggtttact
     7561 gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct ccgtcgtgat
     7621 ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca gccagtggta
     7681 gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat
     7741 cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc agaacctgag
     7801 taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc gtatgcaggt
     7861 aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg
     7921 ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accaaaaatt accgcgcccg
     7981 ccgggcacac ttctacgcag ccaggcaaac cgcctttatc gagacgttcc acacctttct
     8041 ggttgcacaa ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg tacttcggca
     8101 cgttgtatgg gcaggcgacc atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat
     8161 ggacaatgcc ggttttcgga tcttttttca gcgcagagac cgggcacaca gagacacagt
     8221 tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc tcctcctggt
     8281 ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta tacggcgaca
     8341 gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg aagttgatat
     8401 cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc
     8461 ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca gacggcagcg
     8521 cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg ttcacggtta
     8581 ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac gcaccgccat
     8641 cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag cgcctgcaga
     8701 gacgttgccg ccctctttag cgttaacatc cggtttctgt gaacgcggag tctgattttc
     8761 gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt tacagccata
     8821 gcatgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt gcagcgttga
     8881 gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc agccttcacg
     8941 gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc
     9001 gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cgatttttgt catccagttt
     9061 cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg
     9121 cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca ggctgactgc
     9181 gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg aaccgatagc
     9241 gataatcgct gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg gctcaccagc
     9301 aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacaa ccaacacata
     9361 ctgccctttg tacttctcaa gtgcgttatg tttgttctct tcgacctgat gaccgaaggc
     9421 ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt tttctaccgt
     9481 tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg cgccaatcca
     9541 gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag cggctttgct
     9601 acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac gacggttaat
     9661 gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg ttgcgaagac
     9721 ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc catttaccac
     9781 acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac gaagggaaga
     9841 agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt ttcgaggcga
     9901 taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa gaaatccatt
     9961 accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca ggcgctaagc
    10021 tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa accgcgttta
    10081 taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc ttttaatccc
    10141 gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgcta tatatcgcca
    10201 tatatagcga tattttaggc aaagaacgac ttttgcgcac gctatttttt catgcttgtt
    10261 taagacgtaa tccagtaaga gataaaccta tcaggagaga tatttatcga accatttcag
    10321 tgtcctttgc caggcaagat cggcggcaga tttgtcataa cggggcgtgg aatcattatg
    10381 gaatccgtga ttaacccccg gatagatata cgcctcataa accttattat tggctttcaa
    10441 cgccgcctcg taagcaggcc agccctcgtt gattcgggtg tccagttccg cgaagtggag
    10501 tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac cataaaacgg
    10561 caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacgc caccgccata
    10621 gcaaaatccg gtaataccca ctttgcctgc cgcttgcgga tagcgttgca taaactcaat
    10681 tgcggcaaag aaatcattca tcagtttggt tggatcaacc tgctgttgca gctcacgacc
    10741 tttatcatca tttcccggat aacctccaac ggaacttaag ccgtcaggtg ccagggcgat
    10801 ataccccgcc ttcgccactc gccgtgccac atcttcgata tacggattca gtccacgatt
    10861 ctcatgcacc actaccacgg ctggcgtttt gccgctcatt tttgcgggtt tcaccagata
    10921 accccgtacc tcgccgtgac catttggcga aggatacgtg atgtactcag caacaatctc
    10981 cgggtcggta aactctacct gagtcgccag cgcataattt ggcttgagca aatcaaacaa
    11041 cgctaatgcc gtcatcccgc ccaccgcata cttcgccgca aggttgagga actcacgttt
    11101 cgagattttc ccgtgagcgt aatagtcgta gtaatccaac aactcttgtg ggaaatcttt
    11161 ggcggttaaa cgcggcatcg ttgcactcct caactggtgt ttttttaagc aaagcataag
    11221 cacgtgtttt tgcccagttt ttcgtcactc tgtgagccag actacgggat acgcgctggc
    11281 gaatcgctaa actagaaaca ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc
    11341 tggttagcga atcccgaacg ttacgggcag atgcaatacc gctattgcgg aaaaagcggt
    11401 ttacgcctgc ccgcgttatc gctcggttta tggcacaatt tcggtcacgt taacgcgctg
    11461 gaatcacagc gtgcgatcct gcgtaaagcg tttgatttgg gcattacgca ctttgattta
    11521 gccaacaatt acgggccgcc tccaggaagc gcagaagaga actttggtcg cctgctgcgg
    11581 gaggattttg ccgcttatcg cgatgaactg attatctcta ccaaggctgg ctacgatatg
    11641 tggcccggcc cttacggctc tggcggttca cgtaaatacc tgctcgccag cctcgaccaa
    11701 agcctgaagc gtatggggct tgagtatgtc gatatctttt actctcatcg cgtcgatgaa
    11761 aatacgccga tggaagaaac cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg
    11821 ctgtatgtcg ggatctcctc ttactcgcca gagcggacgc aaaaaatggt cgagttgctg
    11881 cacgagtgga aaattccgct tttaattcat caaccttcgt acaatttact gaaccgctgg
    11941 gtggataaaa gcggcctgct ggataccctg caaaataacg gcgtgggctg tattgccttt
    12001 actcctctgg ctcagggatt gctgaccgga aaatatctca acggcattcc gcaagattca
    12061 cggatgtatc gtgaagggaa taaagttcgt ggtctgacac cgaaaatgct taccgaagcc
    12121 aacctcaaca gcctgcgctt attgaatgaa atggcacagc agcgtggaca atcaatggcg
    12181 caaatggcgt taagctggtt gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc
    12241 agccgcgcgg agcaactaga ggagaacgtg caggcgctga ataatctgac atttagcacc
    12301 gaggagctgg cgcagattga tcagcatatc gccgatggcg agctgaatct gtggcaggcg
    12361 tcgtccgata aatgacctgt taataacggg cgcgcgaacc gcgcccgaat cagatcagtg
    12421 attatgacga gtcagtcggt caagatagcc catcacaaat gcagaaagca caaacgtcag
    12481 atggataatg acgtaccaca tcagtttgtt atccgggaca tttttcgcat ccataaagac
    12541 gcgcagtagg tgaatggaag aaattgccac aatcgacgct gctactttgt ttttcagcga
    12601 cgttgcgtcc attttcccca gccagttcag cttctctttg ttctcggaga tatccagctg
    12661 tgagacgaaa ttctcataac cggaaaacat caccatcacc agcaaaccgc caaccagcgt
    12721 catatccacc agcgacagca acacgaggat caaatctgat tccgccatcg agaagatatt
    12781 cggcagtacg ttaataatct cctggaagaa cttcagcgcc agggcaacta acgccagcga
    12841 aaggccaaag tacacggggg caagcagcca gcgagaagca tacattgcat tttcaagaaa
    12901 acgttccata cattcctgtc gtgtagttaa aacgggcaac agtatatcgc aattgagtaa
    12961 acagcacgca acagcaaatc aacatatacg ttaaaccttt gtcgcataat tctgaggttt
    13021 agggatcttc ctggttccct tatcaacgca tcgagagcgt ctacccttaa aggacgttga
    13081 acctgaaggg agaaaaacga tgtctcattt aaaagacccg accacgcagt attacactgg
    13141 tgaatatccc aaacagaaac aaccgacgcc aggcatccag gcgaagatga caccggtacc
    13201 ggattgcggc gagaaaacct atgttggtag cggtcgcctg aaagatcgta aagcactggt
    13261 gacagggggc gattccggaa taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc
    13321 tgacgtggcg atcagttatc ttcccgtgga agaagaagac gctcaggatg tgaaaaagat
    13381 cattgaagaa tgcggacgca aagccgttct gctgccaggc gatttaagcg atgagaaatt
    13441 cgcccgttcg ctggttcacg aagcgcacaa ggcgttaggc gggctggata ttatggcgct
    13501 ggtcgccggg aaacaggttg ccattccgga tattgcagac ctcaccagcg aacagtttca
    13561 aaagaccttt gccattaacg ttttcgcgct gttctggcta acccaggaag cgatccccct
    13621 gctaccgaaa ggtgcaagta tcatcaccac ttcgtcaatc caggcatacc agccaagtcc
    13681 gcatttactg gactatgcgg ctacgaaggc ggcgattctg aactacagcc gtggcctggc
    13741 aaaacaggtc gcggagaaag gtattcgggt gaatattgtc gcgccaggcc ctatctggac
    13801 ggcactgcaa atttccggcg gacaaacgca ggataagatc ccgcagtttg gtcagaaaac
    13861 gccgatgaag cgtgcggggc aaccggcgga actggcccct gtatatgttt atctggcaag
    13921 tcaggagtcg agctacgtca ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg
    13981 ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat tgtgaattca ctcttacctg
    14041 atgtggcgaa aacgccttat ccgcctacaa acgcatgaag attcaacatg ttgcaataga
    14101 ttttgtaggc ctgataagcg tagcgcatca ggcattgagc acctaatgcc ggatgcggcg
    14161 tgaacgcctt atccggccta caaacgcacg aagattcaac atgttgcaat agatattgta
    14221 ggcctgatga gcgtagcgca tcaggcaatt ctactttact tcgctttggc ggtttcttcg
    14281 ccgaccagac ctatcttcag gtaacccgcc tgatgcagcg tatccattac cttcatcaac
    14341 gtctcgtaat cgacggtttt atccgctcgg aagaagatgg tggtgtcttt cttgccttcg
    14401 gttaacgcat tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt accgataaac
    14461 atcgagttgt ctgccttcac cgacagataa accggttttt ccgggcgcgg ctgcggcgtg
    14521 ctggtagaag caggcaagtt caccttcaca tctaccgtcg ctaacggtgc cgccaccata
    14581 aagataatca gcagaaccaa catcacgtcg ataaacggcg tcacgttgat atcatgcatt
    14641 tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt
    14701 aatttttgtg cgacacgaac cggatgcgca gcggcgctgg cttccagatc caggtcacgg
    14761 ctttgcagca acaatacctg cgctgcaaca tcacccagca tcgctttaaa gccgccaatc
    14821 tggcgtgcaa atacgttata gataacgacc gcaggaatcg ctgccacgag gccgattgcc
    14881 gttgctaaca gagcttctgc gatacccggc gcaacgactg ccaggttagt ggtctgcgtt
    14941 tgcgcgatac caataaagct gttcatgatg ccccagaccg taccaaacag accaacgaac
    15001 ggagaaatcg cgccgatggt tgccagatag ccgttaccgc ggcccatttg acgacccact
    15061 gcggcgaccc gacgctccag acggaagctg gtacgttctt taataccttc gttatcgtcg
    15121 ctgccttctg acagttccag ctcattctgc gcttcattga gcaaatgcag gcttaagctt
    15181 ttgctaccaa aatcagcggc gatatcgttg gcctggttta aggaacgcgc ttcagccagc
    15241 agttgctgct cgcgcttaag gcgacgcttc tgattgaaga actctacgct cttactgaag
    15301 aagattgccc aagtgactac ggaggccaaa ataagcccaa tcatcacgca cttaacgaca
    15361 atatcggcgt gctgatacat accccagacg gaaaggtccg tctgcattaa attattaccc
    15421 actgtgtatc tccaggacgc aagtcacaaa atctgcgcat aataatatca aaatgacgtc
    15481 gaattgatag tcgttctcat tactatttgc atactgccgt acctttgctt tcttttcctt
    15541 gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg aaaattttct gctttatgcc
    15601 aattcttcag gatgcgcccg cgaatattca tgctagttta gacatccaga cgtataaaaa
    15661 caggaatccc gacatggcgg acaaaaagct tgatactcaa ctggtgaatg caggacgcag
    15721 caaaaaatac actctcggcg cggtaaatag cgtgattcag cgcgcttctt cgctggtctt
    15781 tgacagtgtg gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg agttgttcta
    15841 tggacggcgc ggaacgttaa cccatttctc cttacaacaa gcgatgtgtg aactggaagg
    15901 tggcgcaggc tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc
    15961 ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc gcctatgaac cgagtcagga
    16021 tttctgtagc aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg atccgctgat
    16081 tggtgccgat atcgttaagc atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc
    16141 aggctccatc accatggaag tccacgacgt tccggcgatt gttgccgccg tacgcagtgt
    16201 ggtgccggat gccatcatta tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc
    16261 gctggatttt ggcatcgatg tttctattca agccgccacc aaatatctgg ttgggcattc
    16321 agatgcgatg attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa
    16381 cgcctatctg atgggccaga tggtcgatgc cgataccgcc tatataacca gccgtggcct
    16441 gcgcacatta ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg
    16501 gctggcagaa catccgcaag ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg
    16561 ccacgaattc tggaaacgag actttacagg cagcagcggg ctattttcct ttgtgcttaa
    16621 gaaaaaactc aataatgaag agctggcgaa ctatctggat aacttcagtt tattcagcat
    16681 ggcctactcg tggggcgggt atgaatcgtt gatcctggcg aatcaaccag aacatatcgc
    16741 cgccattcgc ccccaaggcg agatcgattt tagcgggacc ttgattcgcc tgcatattgg
    16801 tctggaagat gtcgacgatc tgattgccga tctggacgcc ggttttgcgc gaattgtata
    16861 acattgccac ttttggacaa ttttgcagac atttaattgt gaaaagtctt aaattgttgc
    16921 gtccgggatc aaggcgtccc ggacgaatca ggagtacaat aggcagataa aggcttaaac
    16981 gctgttccac aggaaagtcc atggctgtta ttcaagatat catcgctgcg ctctggcaac
    17041 acgactttgc cgcgctggcg gatcctcata ttgttagcgt tgtttacttt gtcatgtttg
    17101 ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt
    17161 tgatactggc aggcgcattg attgcccaag gggttatgga ttttctgcct acgattgcga
    17221 ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta tattcagggg cgctggttag
    17281 ggaataccaa aacggtgaaa ggctggctgg cacagcttcc tgctaaatat caccagcgcg
    17341 ccacctgcat gtttgaccgc cacggtctgc tggcgctgct ggctggacgt tttcttgcat
    17401 ttgtccgtac gctgctgcca accatggcgg gaatttccgg tctgccaaac cgccgcttcc
    17461 agtttttcaa ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt tttggctatg
    17521 ccttaagtat gattccgttc gttaaacgcc atgaagatca ggtaatgacg ttcctgatga
    17581 tcctgccaat tgccttgtta accgctggct tgttaggcac gctgtttgtg gtgattaaaa
    17641 aaaaatactg taacgcctga cgattttccc cgttcccggc tgctgtaccg ggaacgtatt
    17701 taattcccct gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac accgaagtaa
    17761 cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg
    17821 ctggccttca tgccgtcatg gatgatcatc atccgcgcct tatgcagacg gtaattcttc
    17881 aaatactgca acggcgaggt gctggtgaca gacttaaaat tatggtggaa cgccgatacg
    17941 ctcatgttgg cttctgccgc cagttgctcg acgctcaggt tttcggtgta tttattctca
    18001 atccgtttca gcacgcggct aatcagactg aagtgagtct ggcgactgac cagcgccagt
    18061 aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat gatctgtttg
    18121 ccgagaatac gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat
    18181 aaaatctctt ctgataacgt ggcggagtta atcccgctgg ctgccatcga cggctggaaa
    18241 tgctcatctt cgccaatgtc catcaacagt tcctgtaact gcaaaatatc gacattgaga
    18301 cgcaaccctg ccagcggcac ctctgacgtg gcataggttt cgcactcaaa cggcaacggc
    18361 accgtcagca gcaggtattc attggcatca taacgaaaca cgcgttcatt gatataaccg
    18421 attttatgcc cggaaaagag aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg
    18481 aaaggcgtct cgccatacaa caaacgtaca tcgggcaaca gttctgacaa actattttct
    18541 ttaattttca gtttattaac tttatccgcc agcaagcggc aaatctcttc acgtttcata
    18601 tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg cgcaattttg tagcatttct
    18661 ccagcactct ggaggaatag gcaagacatt ggcagaaatg agcattgaga gccagggcgc
    18721 tggcgatcac aatgaaaaac atcaggcaga tcgttctctg ccctcatatt ggcccagcaa
    18781 agggagcaag taatgaacaa ctttaatctg cacaccccaa cccgcattct gtttggtaaa
    18841 ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt gattacctac
    18901 ggtggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc
    18961 atggacgtgc tggaatttgg cggtattgag ccaaacccgg cttatgaaac gctgatgaac
    19021 gccgtgaaac tggttcgcga acagaaagtg actttcctgc tggcggttgg cggcggttct
    19081 gtactggacg gcaccaaatt tatcgccgca gcggctaact atccggaaaa tatcgatccg
    19141 tggcacattc tgcaaacggg cggtaaagag attaaaagcg ccatcccgat gggctgtgtg
    19201 ctgacgctgc cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc
    19261 acaggcgaca agcaggcgtt ccattctgcc catgttcagc cggtatttgc cgtgctcgat
    19321 ccggtttata cctacaccct gccgccgcgt caggtggcta acggcgtagt ggacgccttt
    19381 gtacacaccg tggaacagta tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc
    19441 gcagaaggca ttttgctgac gctgatcgaa gatggtccga aagccctgaa agagccagaa
    19501 aactacgatg tgcgcgccaa cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt
    19561 ggcgctggcg taccgcagga ctgggcaacg catatgctgg gccacgaact gactgcgatg
    19621 cacggtctgg atcacgcgca aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa
    19681 cgcgagacca agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa catcactgaa
    19741 ggttccgatg atgagcgtat tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa
    19801 ttaggcgtgc cgacccacct ctccgactac ggtctggacg gcagctccat cccggctttg
    19861 ctgaaaaaac tggaagagca cggcatgacc caactgggcg aaaatcatga cattacgttg
    19921 gatgtcagcc gccgtatata cgaagccgcc cgctaagctt tttacgcctc aaactttcgt
    19981 tttcgggcat ttcgtccaga cttaagttca caacacctca ccggagcctg ctccggtgag
    20041 ttcatataaa ggaggaacgt atggctaatc caaccgttat taagctacag gatggcaatg
    20101 tcatgcccca gctgggactg ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca
    20161 ttcaaaaagc gttagaagtg ggttatcgct cgattgatac cgccgcggcc tacaagaacg
    20221 aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca
    20281 ccactaagct gtggaacgac gaccacaagc gcccccgcga agccctgctc gacagcctga
    20341 aaaaactcca gcttgattat atcgacctct acttaatgca ctggcccgtt cccgctatcg
    20401 accattatgt cgaagcatgg aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa
    20461 gcatcggcgt gtgcaacttc cagatccatc acctgcaacg cctgattgat gaaactggcg
    20521 tgacgcctgt gataaaccag atcgaacttc atccgctgat gcaacaacgc cagctacacg
    20581 cctggaacgc gacacacaaa atccagaccg aatcctggag cccattagcg caaggaggga
    20641 aaggcgtttt cgatcagaaa gtcattcgcg atctggcaga taaatacggc aaaaccccgg
    20701 cgcagattgt tatccgctgg catctggata gcggcctggt ggtgatcccg aaatcggtca
    20761 caccttcacg tattgccgaa aactttgatg tctgggattt ccgtctcgac aaagacgaac
    20821 tcggcgaaat tgcaaaactc gatcagggca agcgtctcgg tcccgatcct gaccagttcg
    20881 gcggctaaca tgcaaattct cccggtggcg gtaatgttcc gctaccggac ttttcagaaa
    20941 tcatttattc ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga atgccaaata
    21001 taaggacatc atcatgcaga gccggaagct cttaaaagaa caactcatct atatccggga
    21061 taaacgcaac ggagaggtga aaaacagatg aaaataatac ttctgttttt agcagccctg
    21121 gcaagtttta ccgtacacgc acagcccccc tcactgaccg tagaacaaac agtccggcat
    21181 atttatcaga actataaatc agatgccact gccccttatt ttggtgaaac cggagagcgg
    21241 gcgataactt ctgcgcgtat tcaacaggcg cttaccctga acgacaatct tacgctgccg
    21301 ggcaatattg gctggctgga ttatgatccg gtttgtgatt gtcaggattt tggcgatctg
    21361 gtgctagaaa gcgttgcgat aacccaaact gacgccgatc atgccgatgc cgttgtgcgc
    21421 tttcgtatct ttaaagatga taaagaaaag accacgcaga cactgaaaat ggtggcggaa
    21481 aatggtcgtt gggtcattga cgatattgtc agcaatcatg gcagcgtctt acaagcagtt
    21541 aatagcgaga atgaaaaaac gctggccgct ttagcttcgt tgcaaaaaga acagccggaa
    21601 gcctttgttg ccgaactctt tgaacatatt gctgattata gctggccgtg gacgtgggtg
    21661 gtttccgact cttaccgcca ggcggttaat gccttctata aaaccacctt caagacggcc
    21721 aataatcccg atgaagatat gcaaatagaa cggcaattta tttacgacaa tccgatctgt
    21781 tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc
    21841 gattccgccc gtattcatgt tcgttttacg ctgaccaatg gcaacaacga agagcaagaa
    21901 ctggttttac agcggcgcga aggcaagtgg gaaatcgctg attttatccg cccgaacagc
    21961 ggcagcctac ttaagcagat tgaggcaaaa accgccgcca gattaaagca atgagctgaa
    22021 ttaaataaca attagccgga acaataaata aaagggaaca ctatatgaaa acgattttca
    22081 ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg
    22141 tcaatcagct ggcgagcaat gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg
    22201 atatgaaagc actacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata
    22261 cgcgtcttga tgctcaggac tattttgatt gcctgcgctg cttgcgtatg tacgcagaat
    22321 gataaaaaaa tccccggcag catgtcagtt gccggggatt ttttttaacg tccaaccgcc
    22381 gctttagggc gtttcttcgc accagcattc accggacgag attgcgtaga cgacgctttt
    22441 tttgccgtag caggcgtctg acgctgggtc gccatcggcg tatgtttcgt caacgccgga
    22501 cgggtattgc ggttctggcg acgagcttca cgcatctctt caatggttgg cgcaggcact
    22561 aagcaatcgc gacggctgcc aatcagatgc tttttgccca tcgcttccag cgcctggcgg
    22621 attaacggcc agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt
    22681 ttgtcgccct tcggtacgaa gacgtcttcg ctcttataac caatcttcgc cagcgggttt
    22741 ttcccggtgt aatacatggt ggttgagttc gccagcggcg atgggtagaa gttctgtacc
    22801 tggtcgagac ggaaacgatg ctttttcagc cacagcgcca gattcaccat atcttcatca
    22861 cgcgtaccgg ggtgcgcgga gatgaaatag gggatcagat actgctcttt acctgcctgt
    22921 tttgagtaag tatcgaacag ctctttaaag cggtcatagc tgcccatgcc cggcttcatc
    22981 atcttcgata acggcccttc ttcggtatgt tccggggcaa tcttcagata accgccgacg
    23041 tgatgggtcg ccaactcttt gatatagcgc ggatcttcca cggcgatgtc ataacgtacg
    23101 cctgaggcga tgaggatttt tttaatgcct ttcagatcac gcgcacggcg atagaggttg
    23161 atcgtcggtt cgtggttagt gtccatgtgc ggacaaatat ccggataaac gcacgacaaa
    23221 cggcgacaag tttgttcagc gcgtggcgat ttgcagcgca acatatacat gttggcagtt
    23281 ggcccaccga gatcggaaat tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg
    23341 atctcattaa tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg
    23401 atagaacaga aagagcagcc gccaaagcag ccgcgcataa tgttgaccga gaaacggatc
    23461 atttcgtaag ccggaatacg ggcattgcca taggccggat gcggcacgcg cttgtacggc
    23521 agcgcaaaaa cgctgtccat ctcttcggta gaaagcggga tagcaggcgg gttgatccac
    23581 acatagcggt caccgtgttt ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg
    23641 tgcagaatgc gcgaagcatg agcgtacagg actttatcgc ccttcacttt ctcgaaagaa
    23701 ggcagcaata cgtaggtttt ttcccacggt ttcgggcgcg gcggctgcac ggttacagct
    23761 ttcgcttcct gctttttcgg cgcaacaggt ttgttatccg cgcacggcag atcttcgcca
    23821 tacggatgcg ggattgggtc gattttccct ggggtatcaa gacgagtaga atccacaccg
    23881 ctccagccag gcagcgcctc ttttacgata attgcggtat tacgcacatc gcggatttcg
    23941 ctaattggct cgcccatcgc cagacgatgc gctacttcca ccagcggacg ttcaccgtta
    24001 ccaaacatca gcatgtcggc tttcgaatcc accagcacgg aacggcgcac ggtatcggac
    24061 cagtaatcat aatgcgcggt acggcgcaga ctagcctcaa taccgccaag gatcaccggc
    24121 acatctttcc acgcctcttt acaacgctgg gtataaacca gtgtggcgcg atccggtcgc
    24181 ttacccgcga cgttatccgg tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta
    24241 tagcggttga tcatcgaatc catgttgcca gcagtaacac caaaaaacag attcggttta
    24301 cccagacgca taaagtcgtc tttgctgctc cagtccggct gggcgatgat cccgacgcga
    24361 aagccctgcg cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca
    24421 tacgcgtcgc cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct
    24481 tcacgagaca tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag
    24541 gagaacaggt cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg
    24601 ataaaaaaat aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaggta
    24661 ttccatactc gccctcctcg ggcgagtatg aagattacgg taccggattg accaacagtt
    24721 gcccaatcga gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt
    24781 gcgcccagga aggctgaccg aaataaacaa gcagctccac ttgtccgtca acccaaacgg
    24841 tatctttcca gcctctgtct tccgggaacg gcatcgcgcc attcacgtta cggatctgga
    24901 acatcacgcc ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc
    24961 acgttccttg ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt ccattaatac
    25021 ccgggtcatc acccagacta atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag
    25081 ttggcagcaa gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca
    25141 gcgttagcac cagggtagaa accaaaatgc tggatggctc aaagaagcca cgaatacgat
    25201 caacaatgct cgccgcttcg ccacaggtga tcgacacttc atcaccgttg ctcatatcca
    25261 ccagaatctc gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat acaggagcag
    25321 gcaggaatcc ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt
    25381 gatagcgacg agagttcgac gcgttcagca gtcgcaagcg cacccagcca cgcgagactt
    25441 caacgtacgg gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc
    25501 ccggttcgtt gtattctggc gtaccaaagt tatccagccg tttatcctgg ataatgaccg
    25561 gaaaatcatc cacaccataa tggttgggga taggcagcga cttgctgact tcatcttcca
    25621 ccagccacat tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg
    25681 cgtgatacca cagagtagct gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt
    25741 ttggcgacat catccgtgcc ggaccgccca tcagcggacc tggtacctgt agcccggcca
    25801 ccgtcattga gacattttct gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct
    25861 tccagacgcg gatagtcggc cccaggtaac gaccattgat tccccagacc gacgcgcgtg
    25921 tccctggcgt aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc
    25981 gagattcaag tagcggcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca
    26041 ggggaacagc gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga
    26101 gtgacatatt tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgttcccc
    26161 gcaaaaatac agattgccga cttaaacttt tccggcggct tcgcgttctg cgacttcttt
    26221 atcgagctcg gcgatttttt gttccattat cgaacgacaa tgggcagcca gctcacgaac
    26281 ctgatctttg ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc
    26341 gttgtgcagt cgattaagat taatcttatt cgaagttgta gagacgcaca cgggaataat
    26401 cgggacgccc gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg
    26461 accacggctg cgggttcctt ccgggaacat ccagatggaa atacggcgtt ttttgaaatg
    26521 attcacgact tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa
    26581 gttgccggtt aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttacc
    26641 taccgtcacc gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat agttgttctg
    26701 gtggttagcg atatagatag cattgccgta gctttcagca tctgcaggtt tgcggcactc
    26761 aactttcagg ccgaacagcg gcgcaagacg gccaaacata tgcccaaagg tggccacatg
    26821 tttcgggtta cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct
    26881 gtaaatcacg gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag
    26941 agactggcat tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg
    27001 aaaagcgtat tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg
    27061 tttccggggg ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc
    27121 aagcgggagg aaacaggccc tccccggcat attactcttc gctatcaccg ctgctggcac
    27181 ggcgaggaga gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc
    27241 ggcgtccacg ttcgccagtg actttctgta actcttccgg gcgcagttta attttgcgtt
    27301 tcccaacatg aatggtcagc gtgctttgcg gcggcagaac gtacaattgc gccagaccat
    27361 cttctccacg cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg
    27421 acagctgcgg cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca
    27481 gcagcatatc ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta
    27541 aggtgatcaa agccttacct gcacggttac gcgccaccag atcgttaaag gtgcagacga
    27601 aaccgtaacc cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat
    27661 ggtcaacggt cgccccaggc ggcaacgtta atttgccggt gagtggttcg ccctgaccac
    27721 gcgccgacgg cagcgtaatc gggtcgatgg catagctacg accggtggaa tcaacaaaca
    27781 ctaccggttg gttgctctta cctttcaccg ccgctttgaa gctatcaccc gctttataat
    27841 tcaggcccgg cgcgtcgata tcatggcctt tagcgctgcg tacccagccc atctgcgaca
    27901 gcacaatggt gacaggttca gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt
    27961 cgcgttcctg caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt
    28021 ctttcttcag caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc
    28081 gctctttttc cagctcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat
    28141 gacgcagttt cagttcgagg atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca
    28201 tcagcgccgg tttcggttca tcttcgttac gaatgatctc aatcacttcg tcgatgttga
    28261 gaaacgccac cagcaaacct tccaggatat gcaggcgctt gaggactttc tccagacgat
    28321 agttcagtcg gcggcgcacg gtatcgcgac ggaacaccag ccattcggag aggatttcca
    28381 gcaggttttt caccgccgga cgaccatcca gaccgatcat attgaggtta atgcgatagc
    28441 tcttttccag atcggtggta gcgaagaggt ggttcatcac ctgatccata tccacgcggt
    28501 tggaacgcgg cacaatcacc agacgggtcg ggttctcgtg gtcagattca tcgcgcagat
    28561 cgtcaaccat cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg
    28621 cacctgaaac ctgatgcggt aatgcgctga taaccaccgc gccatcttct ttcttccaca
    28681 ccgcgcgcat acgcactgaa ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg
    28741 aagtgataat ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgct
    28801 cgagcgtggt tttcggctgg tcgattaatg cgattgccgc ctgagctact tcacgcaggt
    28861 tatgcggtgg aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa
    28921 tgtttggcag acgagcaggc agcattttcg gctcctgcaa agtgccgtcg aagtttggca
    28981 cccagtcagc cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc
    29041 gggattcggt gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc
    29101 cctgaccatc aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt
    29161 cataacaggc gctatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg
    29221 ccgatttttt aaatttggcg ctggcattca ggcccagttc agacatcgca tacacaatgc
    29281 ggcgctgaac aggtttaaga ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt
    29341 acatggagta gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca
    29401 tatcgctcat taattctgat tcctcaactt attcgccagc cttcaaaggg gcaatattgc
    29461 cggagatact acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc
    29521 ggacgtgctc cgacggcgat aaaagcatca atgtgccttg atatacaact ctttgctgta
    29581 gacttgatcg gcatagaggg cagcacaagg ttgcgcccat gccacaccac tcggcatggg
    29641 ggtccgctcc gggcgattcg agaagttgag gttatgcgag tccagggaag cctaaagcat
    29701 caaagtagtc gactgtaagt atacggttca gcccagttta gctgtgtctc caccagtgcc
    29761 ccgcgcctcc tgccataagt gcggcttgct catggatacc cccgagacgg cttacggcgc
    29821 accggataat aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac
    29881 aacaatacgc ctgtggcatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc
    29941 ggtaaagatt ccccatcacg ccaacggcac ataaacatcc gtttccagtc gctgctctgt
    30001 caccccttcg gcaagattgg tgtagtgaaa cataatcggt gctttacgca ttttctcgcc
    30061 gcttgcaggc agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc
    30121 gccaacgtgg cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata
    30181 acgattatcg ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg
    30241 tgcggtggtg gcagggtcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga
    30301 aagccccgtc tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg
    30361 gctacagcgg tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc
    30421 cagtgtcagg tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga
    30481 gcggtaacaa ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc
    30541 agtctttatc gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt
    30601 tccagcgttt gtggtcgata tcaacattga tagtaccgct ggcatcttta aacacgtaga
    30661 gatcgtcaga gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca
    30721 gggattttgc gctttctacg gtcgttacgc tgccgttcgg cccctggaat cctccggcct
    30781 gactttgcgt tgccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcgc
    30841 tgcacagggc cattactgcg attactgctg cgaatttttt catgtttatt actcccttta
    30901 atgtctgttt ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata
    30961 aaatttagtg ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc
    31021 gcagggatga aaaaatgcga attttactga tagaagatga catgctgatt ggcgacggca
    31081 tcaaaacggg ccttagtaaa atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg
    31141 gaaaagaggc gctttatagc gcgccttatg atgcggtgat cctggattta accttaccag
    31201 gcatggatgg tcgcgatatt ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac
    31261 tgatcctgac cgcgcgcgat gcgctggagg aacgtgtaga agggctgcgt ctgggagctg
    31321 acgattatct gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaagctctga
    31381 tgcgccgaac caacggccag gccagcaacg agctgcgcca cggtaacgtc atgctcgacc
    31441 ccggcaaacg tatcgccacg ctggctggcg aacccttaac actgaaacca aaagaatttg
    31501 ccctgctgga attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag
    31561 agaaactgta tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc
    31621 atctgcgacg caaactcggt agtgatttta ttcgtaccgt gcatggtatt ggctacacat
    31681 taggtgagaa atgaaattta cccaacgtct tagtctgcgc gtcaggctga cgctaatctt
    31741 tttaattctg gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac
    31801 ggataacgtc gatgaattgt tcgacaccca actgatgctg tttgccaagc ggttaagtac
    31861 gctcgatctc aacgaaatca acgcggcgga tcgcatggca cagacgccaa ataaattaaa
    31921 acacggtcat gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat
    31981 ggtccttaac gatggcgata acggagaaga tattccttat agctatcaac gggaaggttt
    32041 tgctgacggg caactggtgg gcgataaaga tcaatggcgt tttgtctgga tgacctcacc
    32101 tgatggcaaa tatcgcatcg ttgttggtca ggaatgggaa taccgtgaag acatggcgct
    32161 ggcgattgtt gccgggcaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat
    32221 catgatggta ctattgggtc gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg
    32281 tatgcgtgac cctgactcgg aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg
    32341 tccgttggtt gagtcgctaa atcaactgtt cgcccgcaca catgcgatga tggttcgtga
    32401 acgacgcttt acctccgacg cagctcacga actacgtagc ccgttaacgg cgctgaaagt
    32461 gcaaaccgaa gttgcgcagc tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct
    32521 tcaattacat tacgggatcg atcgcgctac tcgtctggtt gatcaactgc tcactctatc
    32581 gcggctggac tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct
    32641 gcaatcgtcg gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact
    32701 gacactcaat gcccacggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt
    32761 gcgaaatttg ctggataacg ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac
    32821 gctgaatgct gataatttca tcgtgaggga taacggcccc ggtgtgacgc cagaggctct
    32881 ggcgcgaatt ggcgaacgct tctatcgccc ccccggacaa accgctaccg gcagtggact
    32941 tgggctatcg attgtccagc gaatcgccaa attgcatggc atgaatgttg agtttgggaa
    33001 tgcggaacaa ggtggatttg aggcgaaggt aagctggtaa cttctctgtc tatgagagcc
    33061 gttaaaacga ctctcataga ttttactaat agcaaaatat aaaccgtccc caaaaaagcc
    33121 accaaccaca aaccagacaa ataccgagcg agaatacata tacccaaaac tcgaacattt
    33181 cccgcataaa gagttttctt aagataagaa taataagtgg cgtaagaaga aaaaatgctg
    33241 caatcattgc ttgcaatata cgatcttcgc ctgaaacgga aggtatactg caaatattgt
    33301 ctcccgactc aagcatccag tcatacttat tgaaagcaaa aaaagcatct attaaccaat
    33361 aagtaagcat tatgtataac agagcttcaa atattgtttg aattttatgc tttaacatat
    33421 atcagtacgc tcataaaaat ccatttataa ttatcaacat gacacgttga tattttcatc
    33481 ttttacttat tcaaagccat attttcaatc ccaaaaaaaa ccgaaccaaa cgtgtaaatt
    33541 attccggtgc gagtaagaat tatgaggaat ggctatcagt attgtcattt tcagaaaata
    33601 tttatcctgc atcggtgagt cagagcaaga tcagactttt gctaaattcg caaaagactt
    33661 tgcacatttt gctaatttca ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc
    33721 accttgaggt taaaaaatga gcaacatcct gattatcaac ggcgcgaaaa aattcgccca
    33781 ctccaatggt caactgaacg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct
    33841 cgggcatgat gtccgcatcg ttcgcgccga cagcgactac gatgtcaaag cggaagtaca
    33901 aaactttctc tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc
    33961 gtggacagtg aaaaaatata ttgatgatgt attcaccgaa ggtcatggaa cgctgtatgc
    34021 cagcgatggc cgtacccgca aagatccgtc aaaaaaatac ggttccggcg gcctggtaca
    34081 gggcaaaaaa tatatgcttt ctctgacctg gaacgcacca atggaagcct tcaccgaaaa
    34141 agatcaattc ttccacggcg ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa
    34201 ccaattcctt ggcatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc
    34261 tgatgttccc cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact
    34321 agagctcagg ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg
    34381 tcctggtcaa catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt
    34441 actgaaagaa gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag
    34501 tttccagtct atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca
    34561 tcttgaagcg catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggcga
    34621 cgtgctggag atgaatatcc gcatcctgca acctgggatt taaacctgcc ttgtttgccc
    34681 ggccatcctg accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt
    34741 ctcttgcaac cagttgcggc gatcttccga gcgtttcttc gccagcagca tatccatcat
    34801 cgcgtcagta cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt
    34861 cggatcaagc gtggtttcac gcaattgcat cgggttcatt tcccccagac ctttaaaacg
    34921 ctggacgttc ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc
    34981 ttccgtcagc gcgtaataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc
    35041 gacgtaaacg tgaccgtgtt tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag
    35101 cagcgtggca atgtgcagac catcagagtc cgcatccgcg aggatacaga ttttgccata
    35161 acgaagctgg ctcagatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcatg
    35221 cacttcctgc gaggccagca cttcgtcgga agaaacttcc caggtgttaa ggatcttacc
    35281 tttcagtggc atgatcgcct gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc
    35341 ggagtcacct tccacaagga acagttcggt acggttaaga tcctgcgcgg tacagtcagc
    35401 cagtttgcca ggcagtgctg ggccgctggt cagctttttg cgcaccactt ttttagccgc
    35461 acgcatacgg cgctgggcgc tggaaatcgc catctccgcc agcagctccg ccgcctgaac
    35521 gttctggttc agccacagga taaaggcatc tttcaccacg cctgaaacga atgccgcgca
    35581 ttgacgcgaa gagagacgct ctttggtctg cccggcaaac tgcggatcct gcattttcac
    35641 cgacagaaca taggcgcagc gatcccagat atcttccgcc gacagcttca cgccgcgcgg
    35701 cagaatgttg cggtattcac agaactcacg catcgcgtcc aacaggccct gacgcagacc
    35761 attaacatgg gtaccgccct gcatcgttgg gataaggttg acgtagcttt cggtcagcag
    35821 ttcaccgcct tccggcagcc acagtagcgc ccagtccaca gcttcagtat cgccagcgaa
    35881 attaccgata aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta
    35941 atcattcaga ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa
    36001 ggtaatctca acgccggggc acagtactgc tttggccttc agcacatgcg tcaggcgtga
    36061 aacagaaaaa cgcgggctgt caaagaaggt ttcatccggc cagaagtgca cgctggtacc
    36121 ggtattgcgt ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc
    36181 aaaggcgatg ttataaacct gaccatcgcg gcgcacgtta acttctacgc gcttcgacag
    36241 ggcgttaacc accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt
    36301 agagaattta ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtacaccctc
    36361 ttccgggtga atatccaccg gcatcccgcg cccatcgtca ataacttcta acgactggtc
    36421 agcatgtaag attacatcca cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt
    36481 atcaataact tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg
    36541 gcggcgaacc ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt
    36601 catggtttaa gttagcaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga
    36661 cgatcgggtt gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag
    36721 tctggcggca ggaagcgtag tacgccaccg cctggcggta atccagcact tcatctcccg
    36781 tctgttgcag cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttga
    36841 gatcgtaaat atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct
    36901 gaccgagata gtccgtcagc agttcaaacg ggcgcaccgc cgggtttacc accactgcgg
    36961 gcagcataaa acattgtgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa
    37021 tacccagcga atcgccgcca tgttccagga caatggattc cagcagctct gccgcgtcgg
    37081 aaggatacgg cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc
    37141 agttttttaa caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa
    37201 gaagcgtaga catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag
    37261 gcgatgcacc tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg
    37321 cgcgatggtg tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg
    37381 cgttgccagc aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca
    37441 cagcaagtat ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg
    37501 caggctgtgt tgatcaagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag
    37561 caacgtatgg cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc
    37621 gctcagctca ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg
    37681 ctcaccaata aacacccgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat
    37741 cgcgggctgg aaatcgtggt tgccaggcag ccagacgcag ggcgcacgaa aacttgcgat
    37801 gccttcagcg aaatgccgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc
    37861 gacaatcagg tcgaattcgt gctggtgtgg acgaatcgcc tccagcaccg cctggtagct
    37921 ttcccaggta tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt
    37981 aatttgtaaa atcctgactc tggcctcacc agccagagga agggttaaca ggctttccaa
    38041 atggtgtcct taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa
    38101 acagtatcgc aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg
    38161 caacttttta ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca
    38221 cacttcagcc accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta
    38281 actgatcgcg ggcgcagtct gttctattgt caccagggta gtgtatcggg tcgattccac
    38341 aatcgtcagc cgatattgtg cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc
    38401 attgcgcggt aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc
    38461 aggaaagtca ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttgatgatg
    38521 cagctgcagc cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac
    38581 ccactggtat gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc
    38641 cagaccatga ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg
    38701 ctcactggtg ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat
    38761 cagtcccgcc tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc
    38821 tatcatcccg gcaaccatct ccagtagcca gggggtttcg ctggtgtcgt acgcggcaat
    38881 ccgaatctgc tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc
    38941 ggcgtgaccg cgctcaaaaa tttcccgccg tacctcatga ctcatttgcc cgttgaatag
    39001 acgatgacga aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc
    39061 aataatttct acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt
    39121 taatgtcctg gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt
    39181 gagcagtcat gtgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt
    39241 tgcggtagcg gattctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact
    39301 gcttcaccac aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt
    39361 tctgggttca gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc
    39421 cttagtaacc cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt
    39481 aatgaagcgc gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc
    39541 aacggctacc gcgacgcgaa cggcatcaac tctaacgcga ccagtgcgtc cctgcagtta
    39601 actcaatcca tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca
    39661 gggattcagg acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc
    39721 gcttatttca acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa
    39781 gcgatctacc gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgata
    39841 accgacgtgc agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca
    39901 cgcaataacc ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatcca
    39961 gaactggcag cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg
    40021 ctgctgaaag aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac
    40081 ctggcgcgcg agcaaattcg ccaggcgcag gatggtcact taccgacgct ggatttaacg
    40141 gcttctaccg ggatttctga cacctcttat agcggttcga aaacccgtgg tgccgctggt
    40201 acccagtatg acgacagcaa tatgggccag aacaaagtgg gcctgagctt ctcgctgccg
    40261 atttatcagg gcggaatggt taactcgcag gtgaaacagg cacagtacaa ctttgtcggt
    40321 gccagcgagc aactggaaag cgcgcatcgt agcgtcgtgc aaaccgtacg ttcctccttc
    40381 aacaacatta atgcatctat cagtagcatt aacgcctaca aacaagccgt agtttccgct
    40441 caaagctcat tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat
    40501 gtgttggatg caaccaccac gctgtacaac gctaagcaag agctggcaaa tgcgcgttat
    40561 aactacctga ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat
    40621 ctgctggcac tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc
    40681 ccgcaaacgc cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc
    40741 gtcgttcagc aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac
    40801 tgatgacgac gacggggctt cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt
    40861 atctgccgca ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg
    40921 ggtatttacc actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca
    40981 tcgttccgca aaaactggag cgcgcgccat ctgacaccag tcgctctcgc ggttgccact
    41041 gtttttatgc tggctggctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct
    41101 gacgactgtt cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat
    41161 gcgctgaaag aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct
    41221 gaatttggtg aaggtcagtg ccagcaggca ccagcccagg ctggcatggc accagaaaac
    41281 caggcgcagg cccagcaatc cagcgggagt ttctggatgc cgctgatggc cggttacatg
    41341 atggggcgtc tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa
    41401 aacccagcca gcccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca
    41461 gcccagccag gccgcaccat gaccgtaccg aagacagcca tggcaccaaa accggcgacc
    41521 accactaccg ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag
    41581 cgtagcgcaa ctggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca
    41641 gtattaccga gcgcccggac tggcgtgaga aagcccacga atacggtttc aattttcaca
    41701 ccatgtacgg cgagccgtac tggtgtgaag atgcttacta caagttgacc ctcgcccagg
    41761 ttgaaaagct ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa
    41821 aagtgatcgc cagcgatgag ctgatgacca aattccgcat tccaaaacac acctggagtt
    41881 ttgtgcgcca gtcatggctg acgcaccagc catcgcttta ttcgcgtctt gatctggcgt
    41941 gggatggcac tggcgaacct aaacttctgg aaaataacgc cgatacgcca acgtcactat
    42001 acgaggcggc gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgc
    42061 cggagggcag cgaccagttt aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc
    42121 tgcgtgaaca gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag
    42181 atcgcggaac cattcagtat ttgcaggact gcgcaacgga agctgaaatt gctactgagt
    42241 tcctctacat cgatgatatc gggttaggtg aaaaaggtca gttcacggat ttacaggatc
    42301 aggtaatttc caacctgttc aagctgtatc cgtgggaatt tatgttgcgt gagatgtttt
    42361 ccaccaagct ggaggatgca ggcgtacgct ggctggaacc ggcgtggaag agcattatct
    42421 ccaacaaggc acttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc
    42481 ccgcttattt tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct
    42541 tctcccgtga aggcgcaaac gtgtcgatca ttgaaaacgg caaaaccatt gaagcagcgg
    42601 aaggtccgta tggcgaagaa gggatgattg ttcagcaatt ccacccgtta ccgaaattcg
    42661 gcgacagcta tatgctgatt ggtagctggc tggtgaacga tcaacccgcc ggaattggca
    42721 ttcgtgaaga ccgtgcattg atcacccagg atatgtctcg gttttatcca catatttttg
    42781 ttgagtaatc aatgatgccg gatggcattc gccatccggt aattgttaac ctatctgcac
    42841 cgacagcata ctcaggctgc ccatttctat accatcgacc ggaatggtaa ttggctcctg
    42901 cccatcccac gcacctaaga catacaacaa cggcagatag tgctccggcg ttgggttcga
    42961 taacgcgcca ccttcatggt cgaggtaatt caccagagga tgctgctcta ccgccccttg
    43021 ccacgtcaga ttcgctttca catactcatt aaacgacatc gcccaaggat atggtgaact
    43081 atcaccgtgc cacttcactg tacgcaggtt atgcaccacg ttgccactgg cgaccaacat
    43141 tattccttca tctcgcagcg ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg
    43201 tttgctactg tcgatactca actgcaccat cgggatatcg gcgtcaggat acatcttaat
    43261 cagcacgccc caggaaccgt ggtcaaagcc ccaggcttct ttatccagcg tcaccgggac
    43321 cggtgctaac agctcaacca gatgctgtgc cagcacaggc gaacccggag caggataatg
    43381 cgtatcgtac agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat
    43441 cgcggtcact cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg
    43501 gcgtggcaat gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac
    43561 gttcatcgga ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat
    43621 atccttaact aaaggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga
    43681 acccggataa caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat
    43741 ttataaggag ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac
    43801 gtttattggc gcgtttctcg gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt
    43861 ttcgctagga tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc
    43921 cgcactagca gctgaaggaa tgtcgcctgt gttgggttat ggaatgttta tcttcggtct
    43981 gcttggctat tttggcctgg accgcatgtt gccacatgcg catccgcagg atttaatgca
    44041 aaaatcggtg cagccgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg
    44101 catcagtctg cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct
    44161 ggagctggga tttggcatcg cactggccgt cgcgttgcac aatattcctg aaggtctggc
    44221 ggttgcaggc ccggtttatg cagcaacggg ttctaaacgc acggcaattc tgtgggcggg
    44281 gatttctgga ttggcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat
    44341 gatttccccc gtggtcatgg cggcaatcat ggcggcggtt gcaggaatta tggtggcgct
    44401 ctcggttgat gaattaatgc cgctcgccaa agagattgac cctaataata accccagcta
    44461 tggcgtacta tgtggaatgt cagtgatggg attcagttta gtgctgctac aaacggcggg
    44521 aattggttaa aagcaacatc agaagttggc cagcgcatgg acaacttctg ctagcttcac
    44581 taaagcctta ttctaccttt tcggctgtct cttctctcgt actgtttaag tatttgttcc
    44641 gcgcttcaag cagtgcgtct ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca
    44701 ggaaataacc caccacaaca agagcaatca atacaatcca gta
//
LOCUS       DAZFJF010000022        44643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFJF010000022 DAZFJF010000000
VERSION     DAZFJF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.022"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            300..386
                     /gene="leuL"
                     /locus_tag="ACHKAU_001409"
     CDS             300..386
                     /gene="leuL"
                     /locus_tag="ACHKAU_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO5530508.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            479..2050
                     /gene="leuA"
                     /locus_tag="ACHKAU_001410"
     CDS             479..2050
                     /gene="leuA"
                     /locus_tag="ACHKAU_001410"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO5530509.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2050..3141
                     /gene="leuB"
                     /locus_tag="ACHKAU_001411"
     CDS             2050..3141
                     /gene="leuB"
                     /locus_tag="ACHKAU_001411"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO5530510.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3144..4544
                     /gene="leuC"
                     /locus_tag="ACHKAU_001412"
     CDS             3144..4544
                     /gene="leuC"
                     /locus_tag="ACHKAU_001412"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO5530511.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4555..5160
                     /gene="leuD"
                     /locus_tag="ACHKAU_001413"
     CDS             4555..5160
                     /gene="leuD"
                     /locus_tag="ACHKAU_001413"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO5530512.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5209..6387)
                     /gene="setA"
                     /locus_tag="ACHKAU_001414"
     CDS             complement(5209..6387)
                     /gene="setA"
                     /locus_tag="ACHKAU_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO5530513.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6489..6620)
                     /gene="sgrT"
                     /locus_tag="ACHKAU_001415"
     CDS             complement(6489..6620)
                     /gene="sgrT"
                     /locus_tag="ACHKAU_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO5530514.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6709..8364
                     /gene="sgrR"
                     /locus_tag="ACHKAU_001416"
     CDS             6709..8364
                     /gene="sgrR"
                     /locus_tag="ACHKAU_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO5530515.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8398..8497
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8528..9511
                     /gene="thiB"
                     /locus_tag="ACHKAU_001417"
     CDS             8528..9511
                     /gene="thiB"
                     /locus_tag="ACHKAU_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO5530516.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDLRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9487..11097
                     /gene="thiP"
                     /locus_tag="ACHKAU_001418"
     CDS             9487..11097
                     /gene="thiP"
                     /locus_tag="ACHKAU_001418"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO5530517.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11081..11779
                     /gene="thiQ"
                     /locus_tag="ACHKAU_001419"
     CDS             11081..11779
                     /gene="thiQ"
                     /locus_tag="ACHKAU_001419"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO5530518.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11893..12657)
                     /gene="yabI"
                     /locus_tag="ACHKAU_001420"
     CDS             complement(11893..12657)
                     /gene="yabI"
                     /locus_tag="ACHKAU_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5530519.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12743..13621)
                     /gene="araC"
                     /locus_tag="ACHKAU_001421"
     CDS             complement(12743..13621)
                     /gene="araC"
                     /locus_tag="ACHKAU_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO5530520.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13960..15660
                     /gene="araB"
                     /locus_tag="ACHKAU_001422"
     CDS             13960..15660
                     /gene="araB"
                     /locus_tag="ACHKAU_001422"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO5530521.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15671..17173
                     /gene="araA"
                     /locus_tag="ACHKAU_001423"
     CDS             15671..17173
                     /gene="araA"
                     /locus_tag="ACHKAU_001423"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO5530522.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17342..18037
                     /gene="araD"
                     /locus_tag="ACHKAU_001424"
     CDS             17342..18037
                     /gene="araD"
                     /locus_tag="ACHKAU_001424"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5530523.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18112..20463
                     /gene="polB"
                     /locus_tag="ACHKAU_001425"
     CDS             18112..20463
                     /gene="polB"
                     /locus_tag="ACHKAU_001425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO5530524.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20628..23534
                     /gene="rapA"
                     /locus_tag="ACHKAU_001426"
     CDS             20628..23534
                     /gene="rapA"
                     /locus_tag="ACHKAU_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO5530525.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23546..24205
                     /gene="rluA"
                     /locus_tag="ACHKAU_001427"
     CDS             23546..24205
                     /gene="rluA"
                     /locus_tag="ACHKAU_001427"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO5530526.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24322..25137)
                     /gene="djlA"
                     /locus_tag="ACHKAU_001428"
     CDS             complement(24322..25137)
                     /gene="djlA"
                     /locus_tag="ACHKAU_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO5530527.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25392..27746
                     /gene="lptD"
                     /locus_tag="ACHKAU_001429"
     CDS             25392..27746
                     /gene="lptD"
                     /locus_tag="ACHKAU_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO5530528.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27799..29085
                     /gene="surA"
                     /locus_tag="ACHKAU_001430"
     CDS             27799..29085
                     /gene="surA"
                     /locus_tag="ACHKAU_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO5530529.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29085..30074
                     /gene="pdxA"
                     /locus_tag="ACHKAU_001431"
     CDS             29085..30074
                     /gene="pdxA"
                     /locus_tag="ACHKAU_001431"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO5530530.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30071..30892
                     /gene="rsmA"
                     /locus_tag="ACHKAU_001432"
     CDS             30071..30892
                     /gene="rsmA"
                     /locus_tag="ACHKAU_001432"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO5530531.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30895..31272
                     /gene="apaG"
                     /locus_tag="ACHKAU_001433"
     CDS             30895..31272
                     /gene="apaG"
                     /locus_tag="ACHKAU_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO5530532.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31279..32127
                     /gene="apaH"
                     /locus_tag="ACHKAU_001434"
     CDS             31279..32127
                     /gene="apaH"
                     /locus_tag="ACHKAU_001434"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO5530533.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32152..32751
                     /locus_tag="ACHKAU_001435"
     CDS             32152..32751
                     /locus_tag="ACHKAU_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO5530534.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32787..33254
                     /locus_tag="ACHKAU_001436"
     CDS             32787..33254
                     /locus_tag="ACHKAU_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530535.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33353..33532)
                     /locus_tag="ACHKAU_001437"
     CDS             complement(33353..33532)
                     /locus_tag="ACHKAU_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO5530536.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33553..34032)
                     /gene="folA"
                     /locus_tag="ACHKAU_001438"
     CDS             complement(33553..34032)
                     /gene="folA"
                     /locus_tag="ACHKAU_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO5530537.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34224..36086)
                     /gene="kefC"
                     /locus_tag="ACHKAU_001439"
     CDS             complement(34224..36086)
                     /gene="kefC"
                     /locus_tag="ACHKAU_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO5530538.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36079..36609)
                     /gene="kefF"
                     /locus_tag="ACHKAU_001440"
     CDS             complement(36079..36609)
                     /gene="kefF"
                     /locus_tag="ACHKAU_001440"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO5530539.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36717..38048)
                     /gene="yaaU"
                     /locus_tag="ACHKAU_001441"
     CDS             complement(36717..38048)
                     /gene="yaaU"
                     /locus_tag="ACHKAU_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5530540.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38107..38394)
                     /gene="fixX"
                     /locus_tag="ACHKAU_001442"
     CDS             complement(38107..38394)
                     /gene="fixX"
                     /locus_tag="ACHKAU_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO5530541.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38391..39677)
                     /gene="fixC"
                     /locus_tag="ACHKAU_001443"
     CDS             complement(38391..39677)
                     /gene="fixC"
                     /locus_tag="ACHKAU_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5530542.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39728..40669)
                     /gene="fixB"
                     /locus_tag="ACHKAU_001444"
     CDS             complement(39728..40669)
                     /gene="fixB"
                     /locus_tag="ACHKAU_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5530543.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40684..41454)
                     /gene="fixA"
                     /locus_tag="ACHKAU_001445"
     CDS             complement(40684..41454)
                     /gene="fixA"
                     /locus_tag="ACHKAU_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO5530544.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41928..43442
                     /gene="caiT"
                     /locus_tag="ACHKAU_001446"
     CDS             41928..43442
                     /gene="caiT"
                     /locus_tag="ACHKAU_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO5530545.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43473..44615
                     /gene="caiA"
                     /locus_tag="ACHKAU_001447"
     CDS             43473..44615
                     /gene="caiA"
                     /locus_tag="ACHKAU_001447"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO5530546.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
BASE COUNT        10812 a        12144 c        11654 g        10033 t
ORIGIN      
        1 aatcgcaaaa catataattc aatacaaatc atcaggatag gttttgcaac gcgtgcattt
       61 tgtccccttt ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg
      121 aaaagcgtcg gtagttaagc agaaattaat atcgcttact ttaaccaccg cagcacaatt
      181 agctaatttt acgggtgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga
      241 catccgtttt tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa
      301 tgactcacat cgttcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta
      361 gacgagtgag cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat
      421 tgcgcgggtt tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat
      481 gagccagcaa gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc
      541 aagcttgagt gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga
      601 cgtgatggaa gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat
      661 cgcccgccag gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat
      721 cgacgtggcg gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc
      781 tacctcccca atgcacatcg ccaccaagct gcgcagcacc ctggacgagg tgatcgaacg
      841 cgctatctat atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga
      901 agatgccggg cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc
      961 cggtgccacc accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc
     1021 cggaatcatc agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt
     1081 acatacccac gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg
     1141 tgcacgccag gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct
     1201 ggaagaagtc atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat
     1261 taatcaccag gaaatatggc gcaccagcca gttagttagc cagatttgta atatgccgat
     1321 cccggcaaac aaagccattg ttggcagcgg cgcattcgca cactcctccg gtatccacca
     1381 ggatggcgtg ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct
     1441 gaaccaaatc cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat
     1501 ggacgagatg gggtataaag aaagtgaata taatttagac aatttgtacg acgccttcct
     1561 gaagctggcg gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat
     1621 cggtaagcag caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg
     1681 ctctaacgat atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga
     1741 agccgccaac ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga
     1801 ctataacgtc gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct
     1861 gggtcaggtg gatattgtcg ccaactacaa cggtcgccgc ttccacggcg tcggcctggc
     1921 caccgatatt gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg
     1981 tgccgcagaa gtcgaaaaag agttgcaacg caaagctcaa cacaacgaaa acaacaagga
     2041 aaccgtgtga tgtcgaagaa ttaccatatt gccgtattgc cgggggacgg tattggtccg
     2101 gaagtgatga cccaggcgct gaaagtgctg gatgccgtgc gcaaccgctt tgcgatgcgc
     2161 atcaccacca gccattacga tgtaggcggt gcagccattg ataaccacgg gcaaccactg
     2221 ccgcctgcga cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc
     2281 ggtccgaaat gggaacattt accaccagac cagcaaccag aacgcggcgc gctgttgcct
     2341 ttgcgtaagc acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg
     2401 gaagcattct gtccgctgcg tgcagacatt gccgctaacg gcttcgacat cctgtgcgtg
     2461 cgcgaactga ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa
     2521 tatgaaaaag cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc
     2581 atcgcgtttg aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac
     2641 gtgctgcaat cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg
     2701 gatgtcgaac tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca
     2761 tcacagtttg acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc
     2821 gcaatgatca ctggctcgat ggggatgttg ccttccgcca gcctgaacga gcaaggtttt
     2881 ggtctgtatg aaccggcagg cggctcagca ccagatatcg caggcaaaaa catcgccaac
     2941 ccgattgcgc aaattctgtc gctggcactg ctgctgcgct acagcctgga tgccgatgat
     3001 gcggcttgcg ccattgaacg cgccattaac cgcgcattag aagaaggcat tcgcaccggg
     3061 gatttagccc gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc
     3121 tatgtggcag aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc
     3181 acgtagtgta cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc
     3241 atgaagtgac ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc ccggtacgtc
     3301 agccgggcaa aaccttcgcc actatggatc acaacgtctc tacccagacg aaagacatta
     3361 atgcctgtgg tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat
     3421 ttggcgtcga actgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc
     3481 cggaacaggg cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca
     3541 cccacggcgc gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac
     3601 tggcaacgca aaccctgaaa cagggccgcg caaaaaccat gaaaattgaa gtccagggca
     3661 aagccgcgcc gggcattacc gcaaaagata tcgtgctggc aattatcggt aaaaccggta
     3721 gcgcaggcgg caccgggcat gtggtggagt tttgcggcga agcaatccgt gatttaagca
     3781 tggaaggtcg tatgaccctg tgcaatatgg caatcgaaat gggcgcaaaa gccggtctgg
     3841 ttgcaccgga cgaaaccacc tttaactatg tcaaaggccg tttgcatgcg ccgaaaggca
     3901 aagatttcga cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt
     3961 tcgataccgt tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca
     4021 atccaggcca ggtgatttcc gtgaacgaca atattcccga tccggcttcg tttgccgatc
     4081 cggttgaacg cgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggcattc
     4141 cgctgaccga agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tcgcgcattg
     4201 aagatttacg cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg
     4261 cactggtggt tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata
     4321 aaatctttat tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga
     4381 tgaacaacga ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg
     4441 aaggccgcca ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg
     4501 ctgctgtgac cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca
     4561 gagaaattta tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc
     4621 gatgcaatca tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat
     4681 ctgtttaacg actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg
     4741 ctgaacttcc cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt
     4801 ggctcctcgc gtgagcacgc gccctgggca ttgaccgact acggttttaa agtggtgatt
     4861 gcgccgagtt ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg
     4921 aaattaagcg atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc
     4981 catttcgacg tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt
     5041 accatcgatg ccttccgccg ccactgcatg atgaacggtc tggacagtat tgggcttacc
     5101 ttgcagcacg acgacgccat tgccgcttat gaagcaaaac aacctgcgtt tatgaattaa
     5161 tccccttgcc cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt
     5221 aacctttgcg gttaaaaata atgcgacaac agaaataacc gcaattaccc agtagacagc
     5281 aaagtgcccc caactttgtg caattgctcc ctgaataacg ccagccagaa ttaccccggt
     5341 agaaatactg ttagtaaata aggtggtagc tgcccccgct cttccaggca ttaaatcctg
     5401 aaaccatagc atcccaatac ccgcaacaat gccgataaat acagcgttaa aaagttgcag
     5461 cgtcatcaac gccatacggc tatgaaagaa aatcaatccg gtgtaaaaca gtactcctgc
     5521 cgccactgct atgaccatca ttcgccgctt accataacgt ttgacatagt agccagccag
     5581 aatcattgct ggtatttcca gtccagctgc cgtccccatc aggaaacccg ccagtttgtc
     5641 tggcaatcct aactcgctac tgatccacaa cggcatatca ataatgtaca tggtgttgca
     5701 ggtccacatt aacgtcgagg cgacaaataa catccgtacg ttactatcct gccagccacc
     5761 ttgcattgat aaagcatttt ccgacggcag ttctacccgc gccacagacg gaagcataaa
     5821 tgcaatcaat accagactga gtgtgaatat cccggcggca atcgaaaaca tcaccgtaaa
     5881 gccgtaattc aacgccagca taaaggccaa cggtggaccg ataacccatg ccagagaaag
     5941 ctgcgcacgc atcaccgagc taaacatcac cacttctcgc gccgagttat ccgcatattc
     6001 ccgcgccaga gcaaataact gtggcattgc cgtattggcc agagatgcca gaagcacacc
     6061 acaggtgata agcgtcagat aatgacgatt aaatgcaaac aatagcgcat tgccgatagc
     6121 catcaaacag caaaatataa tcagttttcg ccgatcgccc tgactgtcag aacgttttgc
     6181 caaccagagg cttacgccga tcccagcaat agcattcacc gtataaaaga gtccgatcca
     6241 gaaaggttgc gcgccaacct cacgactcag aaataagctc aatgtaggag cctgtagcgc
     6301 cccggccacc cccatcataa aagcgaccag cataaatgcc gcgtaaacac cgttcatacg
     6361 gcgagccatc gtcattatcc agatcatacg ttcccttttt agcgcggcga gaataaaaaa
     6421 aaccagcagg tataatctgc tggcgggtga ttttacacca atactcagtc acacatgatg
     6481 caggcaagtc aactttcaga attgcggtca tcccactgca tcagttcttc aagcatggtt
     6541 aatcgttgtg ggacgcttaa ccaacgcaac cagcacaact tcgctgtcgc ggtaaaatag
     6601 tgctgataaa actgacgcat ggggcacctc cttgcttcat cgttaggaat tattgactta
     6661 atatagggaa aataaaattg ctgtcttttg cgcaggagtt ccccttttat gccatctgct
     6721 cgtctgcaac aacagttcat ccgcctgtgg caatgctgcg agggtaaatc gcaggacaca
     6781 acgctcaacg aactggcagc gttattgagc tgctcgcgtc gtcatatgcg taccctgctc
     6841 aacaccatgc aggatcgcgg ctggctgacg tgggaagcgg aagtcgggcg cggtaaacgc
     6901 tcgcgtctga cattcctcta taccgggctg gcgcttcagc aacagcgggc ggaagacctg
     6961 ctggagcagg atcgtatcga ccaactagtg cagttggttg gcgacaaagc gactgtgcgg
     7021 caaatgctgg tttctcatct gggccgcagc ttccgccagg ggcggcacat cctgcgcgtg
     7081 ctctactatc gtccgttgcg taatctgcta cctggcagcg cattgcgccg ttccgaaacc
     7141 catattgccc ggcaaatctt cagttcgcta acgcgcataa atgaggaaaa tggggaactg
     7201 gaagcagaca tcgcccacca ctggcagcaa atatcaccgc ttcactggcg tttctttttg
     7261 cgtccaggag tccattttca tcatggtcgt gaactggaaa tggacgacgt gatcgcctct
     7321 ttaaaacgaa tcaatacgct gccgctctat tcgcatattg cggacattgt gtcgccgacg
     7381 ccctggacgc tggatatcca tctcacgcaa ccggaccgct ggttaccgtt actgctggga
     7441 caagttccgg cgatgatcct gccgcgcgaa tgggaaaccc tcagtaactt tgccagccat
     7501 cccatcggca ccggtccgta tgcggtgatc cgcaacacca ccaatcaact gaaaattcag
     7561 gcattcgatg acttcttcgg ttaccgggca ttaatcgatg aagttaacgt ctgggttctg
     7621 ccggaaattg ccgacgagcc agctggaggt ctgatgctaa aaggtccaca gggtgaggaa
     7681 aaagagattg aaagccgcct ggaggaaggt tgctactatt tactgttcga cagccgcacc
     7741 catcgcgggg cgaatcagca agtcagggac tgggtaagct atgtgctttc tccaactaat
     7801 ctggtctatt tcgctgagga acagtaccag caactgtggt tcccggctta tggactgctc
     7861 ccccgttggc accatgcccg caccataaag agcgaaaaac cggctggcct ggaaagcctc
     7921 atcctgacct tttatcagga tcacagtgag catcgggtga ttgccgggat catgcagcag
     7981 attctggcaa gtcaccaggt cacgctggaa atcaaagaga tcagctacga tcagtggcat
     8041 gaaggagaga tcgagagcga tatctggctt aacagcgcca actttacgct gcctctggat
     8101 ttttcgctgt tcgcgcactt gtgcgaggtg ccgctgctcc aacactgtat tccgatcgac
     8161 tggcaagccg acgccgcccg ctggcgcaat ggcgaaatga acctggcgaa ctggtgccag
     8221 caactggtcg ccagcaaagc aatggtgccg cttatccacc actggctgat cattcagggg
     8281 caacgcagta tgcgcggcct gcgcatgaac accctcggct ggttcgattt taaatcagcg
     8341 tggtttgcgc caccggatcc atgattgctg gtagataaca aaatcactac actaacgccg
     8401 ttctcaacgg ggtgccacgc aaacgcgtgc gctgagaaaa tacccgtcga acctgatccg
     8461 gataacgccg gcgaagggat ttgaggctcc ttctcaagtc ctttgccact cttttttgag
     8521 gtgcaaagtg ttaaaaaaat gtcttcccct gctgttgctg tgcacagcgc ccgttttcgc
     8581 taaacccgtt ctgactgttt atacctacga ttccttcgcc gccgactggg ggcctggtcc
     8641 aaagatcaaa aaagcttttg aagccgactg taactgcgaa ctgaaactgg tggcgctgga
     8701 agatggcgtt tcgcttctca accgtctacg gatggaaggc aaaaatagta aagccgatgt
     8761 ggtgctgggg ctggataaca atctgttaga cgccgccagt aaaaccggac tatttgccaa
     8821 aagcggtgtg gcagcggatg ccgttaacgt tcccggcggc tggaataatg acactttcgt
     8881 cccgtttgat tacggctatt tcgccttcgt ctatgacaag aacaaactga aaaacccgcc
     8941 gcaaagcctg aaagaattgg ttgagagcga tcagaactgg cgggtgattt atcaggatct
     9001 gcgcaccagt acgccggggc tgggcctgct gttgtggatg caaaaagtct atggcgatga
     9061 cgccccacaa gcctggcaaa aactggcgaa gaaaaccgtc acggtcacca aaggctggag
     9121 cgaagcctac ggcctgtttt taaaaggtga aagcgatctg gtactgagtt acaccacctc
     9181 tccggcttat cacattctcg aagagaaaaa agataactac gccgccgcga actttagcga
     9241 aggtcactat ctgcaggtgg aagtcgccgc ccgcaccgct gccagcaagc agccggagct
     9301 ggcgcaaaaa ttcctccagt ttatggtttc tccggctttt cagaatgcga tcccaaccgg
     9361 taactggatg tatccggtgg caaatgtcac gctgcctgcc gggtttgaac aattgaccaa
     9421 accggccacc acgctggagt tcacaccagc tgaagtggct gcacaacgtc aggcatggat
     9481 tagcgaatgg caacgcgccg tcagccgtta attcccggct ggttaattcc aggtgtaagc
     9541 gccgccacgc tggtggtagc ggttgcgctc gcggcgtttc tcgccctgtg gtggaacgcg
     9601 ccgcagggta actgggtggc agtctggcag gacagctacc tgtggcatgt ggtgcgcttc
     9661 tccttctggc aggcgtttct ctcggcgcta ctctctgtcg tacccgcgat attcctcgcc
     9721 cgcgccctct atcgcaggcg ctttccgggt cggctggcgc tgttgcgtct gtgcgcaatg
     9781 accttgatcc tcccggtgct agttgctgtt ttcggcattc ttagcgtcta tggtcgtcag
     9841 ggctggctgg catcgctctg ccaatcgctc ggtctggagt ggaccttttc gccctacggc
     9901 ctgcaaggca ttttgctggc gcacgtattt tttaatctgc cgatggcgag ccgcttatta
     9961 ctccaggcac tggaaaacat tcccggcgaa caacgtcagc ttgccgccca gcttgggatg
    10021 cgcggctggc attttttccg cttcgtcgaa tggccgtggc tacggcgaca aatcccgccg
    10081 gttgccgcgc ttatctttat gctctgtttc gccagcttcg ccaccgtgct atcgctgggc
    10141 ggcggtccgc aggcaactac tatcgagctg gctatttatc aggcgctgag ttacgactac
    10201 gatcctgccc gcgcggcgat gctggcgctg ctccagatgg tgtgttgcct tgggctggtg
    10261 ctgttgagtc agcgactaag taaggccatt gcgcccggta ccacgctgct gcaaggctgg
    10321 cgcgatccgg acgatcgtct gcatagccgc atttgcgaca cggtgttaat agtgctggcg
    10381 ctgctgctgt tgctgccacc gttgctggcg gtgatcgtcg atggggtaaa tcgccagttg
    10441 ccggaagtgc tggcacaacc ggtgctgtgg caggcgctgt ggacctcgtt gcgtattgcg
    10501 ctggcggcag gtgtattgtg cgtagtgctg accatgatgc tgctatggag cagtcgcgaa
    10561 ctgcgggcgc ggcagaaaat gctggcgggt caggcgctgg agatgagcgg catgttgatc
    10621 ctcgccatgc cggggattgt gctggctacc ggcttctttt tactgctcaa caacactatc
    10681 ggcctgccac aatctgctga cggcattgtg attttcacca atgcgttaat ggcgatccct
    10741 tatgcgctga aagtactgga aaacccgatg cgcgatatca ccgcccgcta cagtatgttg
    10801 tgtcagtcgc tgggcattga aggctggtcg cgcttaaaag tggtcgagct gcgcgccctg
    10861 aaacgtccac tggcgcaggc gctggctttt gcctgcgtgc tgtcgattgg tgattttggc
    10921 gtagtggcgt tgttcggtaa cgatgatttc cgtaccctgc cgttttatct ctaccagcaa
    10981 attggctcct atcgcagcca ggacggcgcg gtcaccgcgt taattctgct actactctgt
    11041 tttctgctgt ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac tgactgatat
    11101 cacctggctt tatcaccatt tgccgatgcg ttttagctta acggtagaac gcggcgagca
    11161 ggtggcaatc ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt tgattgccgg
    11221 ttttctgacg ccagccagcg gttcgctgac tatcgacagc gttgatcaca ccacgacgcc
    11281 gccgtcacgc cgcccggtat cgatgctgtt tcaggagaac aacctgttca gccacctgac
    11341 ggtcgcgcag aacatcgggc tggggcttaa tccgggattg aagctgaacg cggcgcagca
    11401 ggagaaaatg cacgctatcg cccgccagat ggggattgat aatttaatgg cgcggttacc
    11461 gggcgagctt tccggcggtc agcgacagcg cgtggcgtta gcgcgttgtc tggtgcgcga
    11521 acagccgatt ttattgctcg atgaaccgtt ctctgcgctc gatccggcgt tacgtcagga
    11581 gatgctgacg ctggtgagca cgagctgcca gcagcaaaaa atgacgctat tgatggtgtc
    11641 gcacagtgtg gaagatgcgg cgcggatcgc cacacgctcg gtggtggtcg ccgacgggcg
    11701 catcgcctgg cagggtaaga ccaatgagtt gttgagcggt aaggcgagtg cttcggcact
    11761 attggggatt acgggttagt atgcactgtc ggatgcggcg tgaacgcctt atccgaccta
    11821 cgggtagtgc gttttgtagg cctgataaga cgcggtcagc gtcgcatcag gcaacccgca
    11881 caagactatc tcctaacccc caaccacttt acgcaaaata tcgatataca ccggcatcaa
    11941 cgggtggcga attaacacca ccagcgccac cacgccgatg gcagaaatta acggcgtcag
    12001 ccacaacaaa cggccgcgcg acaaataatg acttaaacga tcggttgctt taccgctgcg
    12061 ccataaccgc cagcacagcc agccaccaac ccacaaaaat accgccgtcg ccagcagcaa
    12121 ccatttaaac tcgccgctct gcattccggc tggaatatcg atcgccgcgc ccgccagaat
    12181 cccaggcagg aagtaaaacg gtggccacag caggcagccg ataatattcg gcgtaatgaa
    12241 ttttgccacc ggcagatcca gcattcccgc caccattggc accagcggac gcgtcgggcc
    12301 aacaaagcga ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat gttcagtttt
    12361 atcgagtaat gctttgtttt tcttcagaaa tgaccagcga tgcaacggct ttttaaaacg
    12421 ccagcccagc cagaaggaga tccagtcgcc cagcaggcaa ccgacgatcc ctgccagcca
    12481 ggcgtgccag aaacttaact cgccgctgcc aatcagcgct cccagccccg ccatcagcac
    12541 cgtaccgggt agaatcaaac cgaccagcgc cagcgactcc agaaaggcca ccaacaccac
    12601 cgccatcaat gaatacacgg tagattgggt aataaagtgt tccagcaatg cttgcataat
    12661 gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt
    12721 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
    12781 cacaaccggc tcggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
    12841 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
    12901 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
    12961 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
    13021 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
    13081 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
    13141 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
    13201 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
    13261 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
    13321 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggataa cgaccgtagt
    13381 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
    13441 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
    13501 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
    13561 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
    13621 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
    13681 gccgtcactg cgtcttttac tggctcttct cgctaaccca accggtaacc ccgcttatta
    13741 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
    13801 ataatcacgg cagaaaagtc cacattggtt atttgcacgg cgtcacactt tgctatgcca
    13861 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
    13921 ctgtttctcc atacccgttt ttttggatgg agtgaaacga tggcgattgc aattggcctc
    13981 gattttggca gtgattctgt gcgagctttg gcggtggact gcgccagcgg tgaagagatc
    14041 gccaccagcg tagagtggta tccccgttgg caaaaagggc aattttgtga tgccccgaat
    14101 aaccagttcc gtcatcatcc gcgtgactac attgagtcaa tggaagcggc actgaaaacc
    14161 gtgcttgcag agcttagcgt cgaacagcgc gcagctgtgg tcgggattgg cgttgacagt
    14221 accggctcga cgcccgcacc gattgatgcc gacggtaacg tgctggcgct gcgcccggag
    14281 tttgccgaaa acccgaacgc gatgttcgta ttgtggaaag accacactgc ggttgaagaa
    14341 gcggaagaga ttacccgttt gtgccacgcg ccgggcaacg ttgactactc ccgctacatt
    14401 ggcggtattt attccagcga atggttctgg gcaaaaatcc tgcatgtgac tcgccaggac
    14461 accgccgtgg cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt gccagctctg
    14521 ctttccggta ccacccgccc gcaggatatt cgtcgcggac gttgcagcgc cgggcataag
    14581 tcgctatggc acgaaagctg gggtggcttg ccgccagcca gtttctttga tgagctggac
    14641 ccgatcctca atcgccattt accttccccg ctgttcactg acacctggac tgccgatatt
    14701 ccggtgggca ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg
    14761 atttccggcg gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac
    14821 gcactggtaa aagttatcgg tacttccacc tgcgacattc tgattgccga caaacagagc
    14881 gttggcgagc gggcagttaa aggtatttgc ggtcaggttg atggcagcgt ggtacctgga
    14941 tttatcggtc tggaagcagg ccaatcggcg tttggtgata tctacgcctg gtttggtcgc
    15001 gtactcggct ggccgctgga acagcttgcc gcccagcatc cggaactgaa agcgcaaatc
    15061 aacgccagcc agaaacaact gcttccggcg ctgaccgaag catgggccaa aaatccgtct
    15121 ctggatcacc tgccggtggt gctcgactgg tttaacggtc gtcgcacacc gaacgctaac
    15181 caacgcctga aaggggtgat taccgatctt aacctcgcta ccgacgctcc gctgctgttc
    15241 ggcggtttga ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat
    15301 caggggatcg ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc
    15361 attatgcagg cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag
    15421 tgctgtgcgc tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca cgcagacatc
    15481 ccatcagccc agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa
    15541 caggcacaac gctttgaaca gctttatcgc cgctatcagc aatgggcgat gagcgccgaa
    15601 caacactatc ttccaacttc cgccccggca caggctgccc aggccgttgc gactctataa
    15661 ggacacgata atgacgattt ttgataatta tgaagtgtgg tttgtcattg gcagccagca
    15721 tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg ttgttaatgc
    15781 gctgaatacg gaagcgaaac tgccctgcaa actagtgttg aaaccgctgg gcaccacgcc
    15841 ggatgaaatc accgctattt gccgcgacgc gaattacgac gatcgttgcg ctggtctggt
    15901 ggtgtggctg cacactttct ccccggccaa aatgtggatc aacggcctga ccatgctcaa
    15961 caaaccgttg ctgcaattcc acacccagtt taacgcggcg ctgccgtggg acagtatcga
    16021 tatggacttt atgaacctga accagactgc acatggcggt cgcgagttcg gcttcattgg
    16081 cgcgcgtatg cgtcagcaac atgccgtggt taccggtcac tggcaggata aacaagccca
    16141 tgagcgtatc ggctcctgga tgcgccaggc ggtctctaaa caggataccc gtcatctgaa
    16201 agtctgccgt tttggcgata acatgcgtga agtagcggtc accgatggtg ataaagttgc
    16261 cgcacagatc aagttcggtt tctccgtcaa tacctgggcg gttggcgatc tggtgcaggt
    16321 ggtgaactcc atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta
    16381 caccatgacg cctgccacac aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc
    16441 gcgtattgag ctggggatga agcgtttcct ggaacaaggt ggcttccacg cgttcaccac
    16501 cacctttgaa gatttgcacg gtctgaaaca gcttccaggt ctggccgtac agcgtctgat
    16561 gcagcagggt tacggctttg cgggcgaagg cgactggaaa accgccgccc tgcttcgcat
    16621 catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc tttatggagg actacaccta
    16681 tcacttcgag aaaggtaatg acctggtgct cggctcccat atgctggaag tctgcccgtc
    16741 gattgccgta gaagagaaac cgatcctcga cgttcagcat ctcggtattg gtggtaagga
    16801 cgatcctgcc cgactgatct tcaataccca aaccggtcca gcgattgtcg ccagcctgat
    16861 tgatctcggc gatcgttacc gtctgctggt taactgtatc gacacggtga aaacaccgca
    16921 ctccctgccg aaactgccgg tggcgaatgc gctgtggaaa gcgcaaccgg atctgccaac
    16981 tgcttccgaa gcgtggatcc ttgctggtgg cgcgcaccat accgtcttca gccatgcgct
    17041 gaacctcaac gatatgcgcc agttcgccga gatgcacgac attgaaatca cggtgattga
    17101 taacgacacc cgcctgccag cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg
    17161 atttcgtcgc taagtagcca catcaggtat gtaacgcctg atgcgacgct gacgcgtctt
    17221 atctggccta cacgctgcga ttttgtaggc cggataagca aagcgcatcc ggcattgttt
    17281 tccccctcgc ccctttgggg agagggccgg ggtgagggga aacgacctct caggagtcaa
    17341 catgttagaa gatctcaaac gccaggtatt agaagccaac ctggcgctgc caaaacacaa
    17401 cctggtcacg ctcacatggg gcaacgtcag cgccgttgat cgcgagcgcg gcgtctttgt
    17461 gatcaaacct tccggcgtcg attacagcgt catgaccgct gacgatatgg tcgtggttag
    17521 catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc tcctccgaca cgccaactca
    17581 ccggctgctc tatcaggcat tcccctccat tggcggcatt gtgcatacgc actcgcgcca
    17641 cgccaccatc tgggcgcagg cgggtcagtc gattccagca accggcacca cccacgccga
    17701 ctatttctac ggcaccattc cctgcacccg caaaatgacc gacgcagaaa tcaacggcga
    17761 atatgagtgg gaaaccggta acgtcatcgt agaaaccttt gaaaaacagg gtatcgatgc
    17821 agcgcaaatg cccggcgttc tggtccattc ccacggcccg tttgcatggg gcaaaaatgc
    17881 cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc gcttatatgg ggatattctg
    17941 ccgtcagtta gcgccgcagt taccggatat gcagcaaacg ctgctggata aacactatct
    18001 gcgtaagcat ggcgcgaagg catattacgg gcagtaatga ctgtataaaa ccacagccaa
    18061 tcaaacgaaa ccaggctata ctcaagcctg gttttttgat ggattttcag cgtggcgcag
    18121 gcaggtttta tcttaacccg acactggcgg gacaccccgc aagggacaga agtctccttc
    18181 tggctggcga cggacaacgg gccgttgcag gttacgcttg caccgcaaga gtccgtggcg
    18241 tttattcccg ccgatcaggt tccccgcgct cagcatattt tgcagggtga acaaggcttt
    18301 cgcctgacac cgctggcgtt aaaggatttt caccgccagc cggtgtatgg cctttactgt
    18361 cgcgcccatc gccaattgat gaattacgaa aagcgcctgc gtgaaggtgg cgttaccgtc
    18421 tacgaggccg atgtgcgccc gccagaacgc tatctgatgg agcggtttat cacctcaccg
    18481 gtgtgggtcg agggtgatat gcacaatggc actatcgtta atgcccgtct gaaaccgcat
    18541 cccgactatc gtccgccgct caagtgggtt tctatagata ttgaaaccac ccgccacggt
    18601 gagctgtact gcatcggcct ggaaggctgc gggcagcgca tcgtttatat gctggggccg
    18661 gagaatggcg acgcctcctc gcttgatttc gaactggaat acgtcgccag ccgcccgcag
    18721 ttgctggaaa aactcaacgc ctggtttgcc aactacgatc ctgatgtgat catcggttgg
    18781 aacgtggtgc agttcgatct gcgaatgctg caaaaacatg ccgagcgtta ccgtcttccg
    18841 ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg agcacggctt taaaaacggc
    18901 gtcttttttg cccaggctaa aggtcggcta attatcgacg gtatcgaggc gctgaaatcc
    18961 gcgttctgga atttctcttc attctcgctg gaaactgtcg ctcaggagct attaggcgaa
    19021 ggaaaatcta tcgataaccc gtgggatcga atggacgaaa ttgaccgccg tttcgccgaa
    19081 gataaacctg cgctggcaac ttataacctg aaagattgcg agctggtgac gcagatcttc
    19141 cacaaaactg aaatcatgcc atttttactc gaacgagcaa cggtgaacgg cctgccggtg
    19201 gatcggcacg gcggttcggt ggcagcgttt ggtcatctct attttccgcg aatgcatcgc
    19261 gctggttatg tcgcgcctaa tctaggcgaa gtgccgccgc acgccagccc tggcggctac
    19321 gtgatggatt cacggccagg gctttatgat tcagtgctgg tgctggacta taaaagcctg
    19381 tacccgtcga tcatccgcac ctttctgatt gatcccgtcg ggctggttga aggcatggcg
    19441 cagcctgatc cagagcacag taccgaaggt tttctcgatg cctggttctc acgggagaaa
    19501 cattgcctgc cggagattgt gactaacatc tggcacgggc gcgatgaagc caaacgtcag
    19561 ggtaataaac cactttcgca ggcgctgaag atcattatga atgcctttta tggcgtgctt
    19621 ggcaccaccg cctgccgctt tttcgatccg cgcctggcat cgtcgatcac catgcgtggt
    19681 catcagatca tgcggcaaac caaagcattg attgaagcgc agggctacga cgttatctac
    19741 ggagataccg actcgacttt tgtctggcta aaaggtgcac attcggaaga agaggcggcg
    19801 aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt gggcggaaac gctgcaaaaa
    19861 caacggctga ccagcgcatt agaactggag tatgaaactc atttctgccg ttttcttatg
    19921 ccaaccattc gcggagctga taccggcagt aaaaagcgct acgccgggct gattcaggag
    19981 ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg tgcgcaccga ctggacgccg
    20041 ctggcccagc agtttcagca ggagctgtac ctgcgtatct tccgcaacga gccatatcag
    20101 gagtacgtcc gccaaaccat cgacaaactg atggcgggtg aactggatgc gcggctggtt
    20161 taccgtaaac gccttcgccg tccgctgagc gaatatcagc gtaatgttcc gcctcatgta
    20221 cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg gtcgcccctt gcaatatcag
    20281 aatcgcggca ccattaagta cgtatggacc accaacggcc cggagccgct ggactaccaa
    20341 cgttcaccac tggattacga acactatctg acccgccagc tacaacccgt ggcggaggga
    20401 atactccctt ttattgagga taattttgct acacttatga ccgggcaact tgggctattt
    20461 tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg tgacgaatgc tttgccatcc
    20521 agtaccatag cgccctttcc attcctggac ctgaataacg ccactacctc ataagcacgg
    20581 tagcgggtgg ttattgcctg caattaaaga tatagagccg aacacatatg ccttttacac
    20641 ttggtcaacg ctggatcagc gatacagaaa gcgaattggg acttggaacc gttgtcgcgg
    20701 tggatgcgcg aactgtcact ttacttttcc catctactgg tgaaaaccgt ctgtacgcac
    20761 gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg tgataccatt accagccatg
    20821 acggctggca gatgcaagtc gaagaagtaa aagaagaaaa tggcttgctg acctatatcg
    20881 gtactcgcct ggatactgaa gagtccggcg tagccctgcg tgaagttttc cttgatagca
    20941 aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg gcagattgac cgtatggacc
    21001 gctttgcgct gcgttatcgc gcgcgtaagt attccagcga acagttccgt atgccgtaca
    21061 gcggcctgcg cggtcagcgt accagcctga tcccgcatca gctcaacatc gctcatgatg
    21121 ttggtcgccg ccacgcgccg cgcgttctgc tggctgacga agtgggttta gggaaaacca
    21181 ttgaagccgg gatgatcctg catcagcaac tgctctctgg cgctgctgaa cgtgtgctga
    21241 ttatcgtccc ggaaacctta cagcatcagt ggctggtaga aatgctgcgc cgtttcaacc
    21301 tgcgttttgc gctgtttgat gatgagcgtt atgccgaagc tcagcacgat gcttacaacc
    21361 cgttcgacac cgaacagctg gtgatttgct cgctggattt tgcccgtcgt agcaaacagc
    21421 gcctggaaca tctctgtgaa gccgaatggg acctgctggt ggtcgatgaa gcgcatcacc
    21481 tggtgtggag cgaagacgcg ccgagccgcg aatatcaggc tattgaacaa ctggcagagc
    21541 acgtgccggg cgttctgctg ctgaccgcaa ccccggaaca gttgggaatg gaaagccact
    21601 tcgcccgtct gcgtctgctg gacccgaacc gtttccacga ttttgcgcag ttcgttgaag
    21661 agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat gctgctggca ggtaacaaac
    21721 tgagcaatga cgaactgaac atgcttggcg agatgatcgg cgagcaggat atcgagccgc
    21781 tgttgcaagc agcaaacagc gacagcgaag atgcccagag cgcccgtcag gaactggttt
    21841 cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt ccgtaacacc cgtaacggtg
    21901 tgaaaggatt cccgaaacgc gagctgcaca ccattaagct gccgctaccg acgcagtatc
    21961 agacggctat taaagtctcc ggcattatgg gcgcacgtaa aagtgcggaa gatcgtgctc
    22021 gcgatatgct ctacccggag cgtatttatc aggaatttga aggtgataac gccacctggt
    22081 ggaacttcga tccgcgcgtt gagtggctga tgggctacct gaccagccat cgctctcaga
    22141 aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca actggagcag gtactgcgcg
    22201 aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat gtcgattatc gaacgtgacc
    22261 gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca ggtactgctg tgctcagaaa
    22321 tcggttctga aggacgtaac ttccagttcg ccagccacat ggtaatgttt gacctgccat
    22381 tcaacccgga tctgctggag cagcgtattg gtcgtctgga tcgtatcggt caggcgcacg
    22441 atattcagat ccatgtgcct tatctggaaa aaactgctca gtcggtgctg gtgcgctggt
    22501 atcacgaagg tctggatgca ttcgagcaca cctgcccgac cggacgcact atttacgata
    22561 gcgtctacaa cgatctgatt aactatctgg cttcaccgga tcaaaccgaa ggctttgacg
    22621 atctgatcaa aaactgccgc gagcaacatg aagcgctgaa agcacaactg gaacaggggc
    22681 gtgatcgcct gctggaaatc cactccaacg gtggcgaaaa agcccaggca ctggcagaaa
    22741 gcattgaaga gcaggatgac gataccaacc tgatcgcctt cgccatgaac ctgttcgata
    22801 ttatcggtat caatcaggac gatcgcggcg acaacatgat cgtgctgacc ccgtccgatc
    22861 atatgctggt gccggacttc cctggcctgt cggaagatgg catcaccatc acctttgatc
    22921 gtgaagtggc gctggcgcgt gaagatgctc agtttattac ctgggaacat ccgctgatcc
    22981 gcaacggtct ggatctgatt ctttctggcg ataccggtag cagcacgatt tcactgttaa
    23041 aaaacaaagc gttgccggta ggtacgctgt tggtggaact gatttacgtg gtcgaagccc
    23101 aggcgccgaa gcagttgcag ctcaaccgct tcctgccacc gacgccggta cgtatgctgc
    23161 tggataaaaa cggcaacaac ctggcggccc aggtggagtt tgaaaccttt aaccgccagc
    23221 ttaacgcggt taaccgtcac accggcagca aactggttaa cgccgtgcag caggatgttc
    23281 acgctatcct tcagctgggt gaagcgcaga tcgagaaatc tgcccgtgca ttgattgatg
    23341 ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct gtctcgtctg gaagcgctgc
    23401 gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc cattgagagc aaccgtcagc
    23461 aggtaatgga aagcctggat caggcaggct ggcgtctgga tgccctgcgt ttgatcgttg
    23521 taacgcatca gtaacggagc cgaaaatggg gatggaaaac tacaatccac cgcaggaacc
    23581 ctggttggtt atcctgtatc aggatgacca cattatggtg gttaacaagc cgagcggttt
    23641 gttgtctgtg ccgggtcgtc tggaagagca caaagatagc gtgatgacgc gcattcagcg
    23701 tgattatccg caggcagaat cggtgcatcg tctggatatg gctaccagcg gcgtgattgt
    23761 agtggcgctg accaaagccg cggagcggga gttaaaacgc cagttccgcg agcgcgagcc
    23821 gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc cccgcagaag gtctggtgga
    23881 tctgccgctg atttgcgact ggccaaaccg cccgaaacag aaagtctgtt acgaaacggg
    23941 taaacctgcg cagacggaat atgaagtggt ggagtatgtg gcggataaca cggcaagagt
    24001 ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt gtgcatatgc tggcgctggg
    24061 tcatccgatt ctcggcgatc gtttttatgc atcaccagaa gcgagagcga tggcaccacg
    24121 tttgttgcta catgcagaga tgttgacgat tacccatccg gcgtatggca atagtatgac
    24181 gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc agttcggtag gcggataagg
    24241 cgttcacgcc gcatccggca attgtgcgcc gattgcctga tgcgacgcgg gccgcgtctt
    24301 atcaggccta cagtttaaaa cttatttaaa ccctttctgc tgctttatca gctcatatgc
    24361 ctgctgaatt tcctgcgctt tctgcttcgc catctccatc atctcaggcg gcaaaccttt
    24421 cgccaccagc ttatcgggat ggtgttcact catcagctta cggtaggcac gtttgatggt
    24481 ggtcgcatca tccgtcggct tcacgcccag cacattacag gcatcttcca gcgtcgggcc
    24541 acgctgcgct tgctgccagt taccaccgcc agattgctgc tgataaccgc cgccaaactg
    24601 tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg gcgcgggaga tccctaattc
    24661 ttcagcaatg acatacagca ccgcccgttc attcgggtgc agtgaaccat cagcaaacgc
    24721 cgcctgaatc tggatctcca gaaacatacg aattaagtca aaacgaccaa agcagacact
    24781 gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct gattttccca ctcggaacgc
    24841 gttttgcgcc gcagtacggg aagcgccatg aaggttcatt cggtccatca actggctggc
    24901 gatatgaata tcagcctccg tgacgcgacc tttggatttg gttaaatgcc ccatcacttc
    24961 aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg ttggcgaacc acgccatttt
    25021 acggctacgg gctttatcaa acatatggcc aattaacagg cctaacacta cgccccaaaa
    25081 gccgccgccc atcagtaagg ccacggccac gccaatgatt tttccccaat actgcatata
    25141 ttccccaaat cgacacacgg atatcagggc tatctcccac aatataaagg tgcttttacc
    25201 gttttccggc tgcggtcaat tgtgggacat cgcctataat ttgcattatc atacctgtca
    25261 ttcaatgccg tgcctaacac cactgacgct attcggacag gattaacact agcgtagaga
    25321 tgacgagtac gttagtctct gaccgtttgt cacgcgcaac gttaccgatg atggaacaat
    25381 aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc caccatgatt gccaccgccc
    25441 tttatagtca acagggactg gcagccgacc tcgcctcaca gtgcatgttg ggcgtgccaa
    25501 gctatgaccg tcctctggta cagggcgata ccaatgactt acccgtgact atcaatgctg
    25561 accacgcgaa aggggactac ccggatgacg ccgtgtttac tggcagcgtg gatatcatgc
    25621 agggtaacag ccgtctgcag gccgacgaag tgcagctcca tcaaaaagag gcaccaggac
    25681 aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt ccattacgac gataaccagg
    25741 tgatcctcaa agggccgaaa ggctgggcga atctgaacac caaagatacc aacgtctggg
    25801 aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa agcggacctg atgaaacaac
    25861 gtggcgaaaa ccgctatacc attctggata acggtagctt tacctcctgt ctgccgggtt
    25921 ctgacacctg gagcgtggta ggtagcgaaa ttattcatga ccgcgaagaa caagttgcgg
    25981 agatctggaa cgcccgcttt aaggtgggtc cggtaccgat cttttatagc ccctatttgc
    26041 agttgccggt gggtgacaaa cgtcgctctg gtttcttgat cccgaacgcc aagtacacca
    26101 ccaccaacta ctttgagttc tacctgccat attactggaa catcgcgcca aatatggatg
    26161 ccaccatcac gccgcattat atgcatcgtc gtggcaacat catgtgggag aacgaattcc
    26221 gctacctctc ccaggcgggc gctggcttga tggaactgga ctatctgcct tcagataaag
    26281 tctatgaaga tgaacacccg aacgatgaca gttcacgtcg ttggttattc tactggaacc
    26341 actccggggt catggatcag gtgtggcgtt tcaacgtcga ctacaccaag gtcagcgatc
    26401 ctagctactt caatgatttc gataacaagt acggttccag tactgacggc tacgcaacgc
    26461 aaaaattcag cgttggctat gcggtgcaaa acttcaatgc caccgtttca accaagcagt
    26521 tccaggtttt cagcgaacag aacaccagta gctactcggc agagccgcag ttagacgtta
    26581 attactacca gaatgatgtt ggtccatttg atacgcgtat ttacggccag gcagtgcact
    26641 ttgttaacac cagagacgac atgcctgaag caacccgtgt tcacctggaa ccgaccatca
    26701 atttgccgct ctctaataac tggggcagca tcaataccga agcgaagttg ctggcaaccc
    26761 attatcagca aaccaatctt gactggtata actccagaaa cacgaccaag ctggacgaat
    26821 ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa aatggtcttt gaacgcgata
    26881 tggaaatgct ggctccgggt tatacccaaa cgctggaacc gcgcgcgcag tatttgtacg
    26941 tgccgtatcg cgatcagagc gacatctata actacgactc gtctctgctg caatctgact
    27001 actctggcct gttccgggac cggacttacg gcggtcttga ccgtattgcc tccgctaacc
    27061 aggtgacgac cggtgtcaca tctcgcatat atgatgatgc tgccgttgaa cgttttaata
    27121 tttccgttgg tcaaatctac tatttcacgg agtctcgcac tggcgatgac aacataacat
    27181 gggagaatga cgacaaaacg ggttcactgg tgtgggcagg cgatacttac tggcgtatct
    27241 ccgagcgttg gggattgcgt ggcgggattc agtacgatac acgtctggat aacgtagcga
    27301 ccagtaactc cagcattgaa taccgtcggg atgaagaccg tctggtacag ctgaattacc
    27361 gttacgccag cccggaatat attcaggcta cgctgcctaa gtactattcc actgctgagc
    27421 aatataagaa tggtatttcg caggtaggtg ctgtcgccag ctggccaatt gccgatcgtt
    27481 ggtccattgt tggggcctac tactacgaca ccaatgcgaa caagcaagcc gactctatgt
    27541 taggtgtgca atacagctcc tgctgctatg caattcgcgt cggttacgag cggaagctga
    27601 acggttggga taacgataaa caacatgcgg tatatgacaa cgcaatcggc tttaacatcg
    27661 aacttcgcgg cctgagctcc aactacggtc tgggtacgca agagatgctg cgttcgaaca
    27721 ttctgccgta tcaaaacact ttgtgatttg ttgatttacc acgtaatccg cagtgcggtt
    27781 aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc ttctcggtat cgccatgatc
    27841 gcgaatacca gtttcgctgc cccccaggta gtcgataaag tcgcagccgt cgtcaataac
    27901 ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt cggtaaaact gaacgctgct
    27961 caggcaaggc agcaacttcc tgatgacgcg acgctgcgcc accaaatcat ggaacgtttg
    28021 atcatggatc aaatcatcct gcagatgggg cagaaaatgg gagtgaaaat ctccgatgag
    28081 cagctggatc aggcgattgc taacatcgcg aaacagaaca acatgacgct ggatcagatg
    28141 cgcagccgtc tggcttacga tggactgaac tacaacacct atcgtaacca gatccgcaaa
    28201 gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc gtcgcatcac catcctgccg
    28261 caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa acgacgccag cactgagctg
    28321 aacctgagcc acatcctgat cccgctgccg gaaaacccga cctctgatca ggtgaacgaa
    28381 gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta acggcgctga tttcggtaag
    28441 ctggcgattg ctcattctgc cgaccagcag gcgctgaacg gcggccagat gggctggggc
    28501 cgtattcagg agttgcccgg gatcttcgcc caggcattaa gcaccgcgaa gaaaggcgac
    28561 attgttggcc cgattcgttc cggcgttggc ttccatattc tgaaagttaa cgacctgcgc
    28621 ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc gccatattct gctgaaaccg
    28681 tcgccgatca tgactgacga acaggcccgt gtgaaactgg aacagattgc tgctgatatc
    28741 aagagtggta aaacgacttt tgctgccgca gcgaaagagt tctctcagga tccaggctct
    28801 gctaaccagg gcggtgatct cggctgggct acaccagata ttttcgatcc ggccttccgt
    28861 gacgccctga ctcgcctgaa caaaggtcaa atgagtgcac cggttcactc ttcattcggc
    28921 tggcatttaa tcgaactgct ggatacccgt aatgtcgata aaaccgacgc tgcgcagaaa
    28981 gatcgtgcat accgcatgct gatgaaccgt aagttctcgg aagaagcagc aagctggatg
    29041 caggaacaac gtgccagcgc ctacgttaaa atcctgagca actaatggtt aaaacccaac
    29101 gtgttgtgat cactcccggc gagcccgccg ggattggccc ggacttagtt gtccagcttg
    29161 cacagcgtga gtggccggtc gaactggttg tttgtgccga tgccactctc cttaccgacc
    29221 gggcagcgat gctcggtttg ccgctcaccc tccgccctta ttcccccaac tcccctgcac
    29281 aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc gctacgtgaa tctgtcactg
    29341 cggggcagtt agcgattgaa aatggacatt acgtggtgga gacgctggcg cgagcgtgtg
    29401 atggctgtct gaacggtgaa tttgctgcgc tgatcacagg ccccgtgcat aaaggcgtca
    29461 ttaacgacgc aggcattccg tttaccggtc ataccgagtt tttcgaagag cgttcgcagg
    29521 cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg cgtggcgctg gcaacgacgc
    29581 atttaccgct gcgcgatatc gcagatgcta tcacccctgc acttttgcac gaagtgattg
    29641 ctattttgca tcacgatttg cgtacaaaat ttggtattgc cgaaccgcgc attctggtct
    29701 gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg tacggaagag atagacacca
    29761 ttattccggt gctcgacgag ctgcgggcgc aggggatgaa actcaacggg ccgctgcctg
    29821 ccgataccct gtttcagccg aaatatctcg ataacgccga cgccgtgctg gcgatgtacc
    29881 acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg gcgcggtgtg aacattacgc
    29941 tgggcctgcc ctttattcgc acatcagtgg accacggcac cgcgcttgaa ctggcgggac
    30001 gtggcgaagc cgatgtcggc agttttatta cggcgcttaa tctcgccatc aaaatgattg
    30061 ttaacaccca atgaataatc gagtccacca gggccactta gcccgtaaac gcttcgggca
    30121 aaactttctc aacgatcagt tcgtgatcga cagcattgtt tctgccatta acccgcaaaa
    30181 gggccaggca atggtcgaaa tcggccccgg tctggcggca ttgaccgagc cggtcggcga
    30241 acgtctggac cagctgacgg tcatcgaact tgaccgcgat ctggcggcac gtctgcaaac
    30301 gcatccattc ttaggcccga aactgacgat ttatcagcag gatgcgatga cctttaactt
    30361 tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt ttcggcaacc tgccttataa
    30421 catctccacg ccgttgatgt tccatctgtt tagctatact gatgccattg ccgacatgca
    30481 ctttatgttg caaaaagagg tggtgaatcg tctggttgca ggaccgaaca gcaaagcgta
    30541 tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg atcccggtac tggaagttcc
    30601 gccgtcagcc tttacaccac cacccaaagt ggattccgcc gtcgtgcgcc tggttcctca
    30661 tgcaacgatg cctcacccgg ttaaagatgt tcgtgtgttg agccgcatca ccaccgaagc
    30721 ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc aacctgttta gcgtcgaggt
    30781 gttaacggga atggggatcg acccggcgat gcgagcggaa aatatctctg tcgcgcaata
    30841 ttgccagatg gcgaactatc tggcggagaa cgcgcctttg caggagagtt aacgatgatc
    30901 aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca ttgaggcaca atcttcacct
    30961 gataatgaac gttacgtttt tgcttatacc gtaaccatac gcaatctggg gcgagcgcca
    31021 gtgcagttgt tggggcgtta ctggctgatc accaatggca atggccgtga aaccgaagtc
    31081 cagggcgaag gagtggttgg cgtccagcca cttatcgcgc ctggcgaaga gtaccagtac
    31141 accagcggtg caatcattga aaccccgctg ggcaccatgc agggtcacta cgaaatgatc
    31201 gatgaaaatg gcgtcccttt cagcatcgac attcccgtat tccgactcgc cgttcccaca
    31261 ctcattcatt aaaagaatat ggcgacatac cttattggcg acgttcatgg ttgttacgat
    31321 gaactgttcg cattgctgca taaagtagaa tttacccctg ggaaagatac cctctggctg
    31381 acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg ttctgcgcta tgtgaaatcc
    31441 ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc tgcatctgct ggcggtattt
    31501 gccgggatca gccgcaataa accgaaagat cgcctgacac cgctgctgga agcgccggat
    31561 gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc tgcaaatcga cgaagagaaa
    31621 aagttggtga tggcccacgc agggatcacg ccgcagtggg atctacagac cgccaaagag
    31681 tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct atcccttctt tctcgacgct
    31741 atgtacggcg atatgccaaa taactggtca ccggaattgc gggggctggg aagactgcgt
    31801 tttatcacca acgcctttac ccgtatgcgt ttttgctttc caaacggtca actggatatg
    31861 tacagcaaag aatcgccgga agaagcccct gccccactga aaccgtggtt tgcgattcct
    31921 ggccctgtcg ctgaagaata cagcatcgcc tttggtcact gggcatcgct ggaaggcaaa
    31981 ggtacgccgg aaggtattta cgcgctggat accggctgct gctggggtgg gtcattaacc
    32041 tgcctgcgct gggaagataa gcagtatttt gtccagccgt cgaaccggca taaggatttg
    32101 ggtgagggag aggcggtcgc gtcttaacga attaccattc agggaggaat aatggctgac
    32161 tattttgaaa tacgtgctga ttacaatcag cacaccatca cgatttatca ggcttacaat
    32221 gatgcaatcg ctgacgtcgc cgtacgtgac gggcgatttg gcgcaccttt ttcattcaac
    32281 cgcatgacct ggattaaacc ctcttttatg tggatgatgg agcgtagcaa ctggggactg
    32341 aaaaaagatc aacaacacat tctcgcaatc cgaatcaaac gcaccttctt tgatacattg
    32401 ctggaacaag cggtgctaac cacgccggaa gcccatgtgt atccacatgc aggaatttgg
    32461 gaaacgttat ttgcacaagc aaatgtgtat gttcagtggg acccagaacg cagcataaat
    32521 ggtaaaaaac tggagcaccg atcattacag cttggtattt ctcgtaactt gatcagtcaa
    32581 tttaacgaag acgcgattgt tgctattgat gatctgacgc cgctggtgcg aaagtgtcat
    32641 aaccttttga taaacggcaa aacaacacag gcgaaatcct tcctgccgcc agaaaaaatt
    32701 tacccagtta gtgcagcagc gagaaaggct ttgggaatga aaaattcctg agacaaatac
    32761 gcacctatta agcggaaaat atctaagtgc gacaaatcaa caccgggctt aataccagag
    32821 aaattattct ggaaatgatt gacgaagcag atgccccccc gtttgctgag cagcggacct
    32881 ggcatcgcag gcgaatcgct tttctatgtg attgcatcag cgttgctgtc gactacggct
    32941 tcttctgttc tgtctctcag cagtcaataa tgaacataac caagatggct gaagatgtat
    33001 ggataacaga aagtaaaacg acccaactca ttgatatttg gcaacaactt aaacagcttt
    33061 atccgccaga aactattccc tacgcccaga agttacccac taaggaattg cttgaactgc
    33121 tactttctag tcctgacgat cttcgaataa ttgaaattcc tgacatcatc ggtgatgttt
    33181 ttgatgacca aacctggatt cgattattca aaaaacactt tgctgatgtt attcagctac
    33241 gatgtgatgt gtaacaatcc aaaatatatc gccagacgat agaccaaaaa gaaagtattt
    33301 agcagatact caacattctg acttctgtac agaaatagca gagtttaaaa acttaaaact
    33361 tccggtactc atcagagaaa catccagttt cttctgcaaa ttcatttaat gccgagatgg
    33421 catcctcgtt tacttcattc cactgtagag tggcctgaag gtttagacct acgcaatgag
    33481 cgcgactcac aaaatcgcgc tcactggtaa aatcaaacgt cttatcgttc aatttgtagt
    33541 cctggcaaaa aattaccgcc gctccagaat ctcaaagcaa tagctgtgag agttctgcgc
    33601 atcagcatcg tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccgggaa
    33661 atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga tacagtttct gcgcttttgg
    33721 caggaactgc tcataaacgc gaccgccgcc aatcaccatg atttctggta cgtcaccaca
    33781 cgccgcgatg gcttcatcca ccgacttcac ccacgttacg cgatcgtccg tacccggttg
    33841 actgctgagg ataatatttt tgcgtcctgg caacggacga ccgattgatt cccaggtatg
    33901 gcggcccata atcacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg
    33961 caggttccac ggcatggcgt tttccatgcc gataacgcga tctaccgcta acgccgcaat
    34021 cagactgatc attgagattt cccgataaaa aaaattgtcg ccactatacg taaagcgtaa
    34081 accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt ttatattctg ctggcgagtc
    34141 cacgctctct ccctggactc gccgcattac aatgaaacaa aaacaaacag ttagctgtaa
    34201 agtgtgattt acgtcactct ttattaggat gagggtttcg tttccggttc atccgccatg
    34261 ttgccggtat gtttaccttc ttcggttccc tgccagccat gtcgttgaat taatgacaga
    34321 tgttcgcggt cctcggtaat gatctcactt aacatcgcgc tggtgcgttt atagaccgcc
    34381 gcgcgggctt tggtgtcgtt ctcaaccatt gccatctctt ccaccatctg aatattaaag
    34441 cggcggaaca catcggcacg ttctcgcgct tcatacggcc ccagacctaa actttccagt
    34501 gccagacgcc cggttttcag cgcaccttcg aaggtttcac gctccggctt ttcaacgcct
    34561 gcctgacgca aacgaatgta gtggtcgaca tcgcgggcgc gggcaataat ctgcaaatgc
    34621 gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg tttgcggatc atcaatagca
    34681 ttaatcagca cttccgcttt cgccgctccg gcagattcca gtaaatccat ccgcgttgca
    34741 tcgccataaa acactttcat accaaattta cgcagcgttt cgatatggtc cggatcgtga
    34801 tcgagcacca ccattttcac cccgctggag agcagtaaac gtccggtaat ctgcccaaaa
    34861 cgaccgaatc cggcgataat cacgcgcggc tgttcttcgt cgatctcatc ggcttcacgc
    34921 gcttcctcag tagaagattg ctcaaggcga ttgaggatca ccagcagaat cggcgttgct
    34981 gccatcgaca gcgccaccgc cagggtcagc gatttcgccc actccggctc cagcacattc
    35041 gccatctgcg ccgcgccaaa taccacaaag gcaaactcac tgccctgccc taacaacacc
    35101 gcaaaccaac gacgctgttt atttggcact tgcaacggtc gggcaatcag ccacagcatg
    35161 gcgattttga tgatgaggaa accgagcagc aaaatgacaa tgcgcaatgg gttttcaagc
    35221 agcgtgccaa agtctatcga catgccaaca ccgatgaaaa acagccccaa cagcaaacct
    35281 ttaaatggtt cgatatcgct ctccagcgca tgacggtatt ccgagcttgc cagcagtacg
    35341 cccgccagaa acgcgcccat cgccatcgac aagccgacct cttccagcag caaaccaaag
    35401 ccaaacacga ggaataacgc cacggcacta aacacttccc gcaagccaga gcgggctaca
    35461 aaacgcagcg ccggacgcgt gacatagcgc cccagcaata ccaccagcac cagcgcaccc
    35521 gccactttta acgccgagag agcaaatgcg cccatcgtcg tcgaggcact gctcgttgcc
    35581 agtagcggaa tcatcgccac cagcgggatc gccgcgatat cctggaacag cagcaccgca
    35641 aaggcactgc gacccatttg cgtcaccatc agattgcgtt cattcatcgc ctgcatggca
    35701 atcgccgtag aggagagcgc cagcgtcatg ccgatcaatt ccgcgacctg ccagcgcagc
    35761 ccaagtaaca tgcagaacag ccccagcagg ccgccgcaaa tcaccatctg caatgcgcca
    35821 ccgccgaaca ctgccgcacg cagcttccac agcctttgtg gatcgagttc gaggccgata
    35881 ataaacagca tcagcaccac cccaatctcg gcaaagtgca gaatagattc ggcatcggtc
    35941 accagtcgca gcccccacgg gccaataatg cagccggcga tcaggtagcc aagtaccgat
    36001 cccagaccaa gacgtaccgc aatgggtaca atcagcgctg ccgaaccgag ataaatcagc
    36061 gcctgaatca gcgtatggct atccatgatg ggcctcctgc cattccagca gacgttgctt
    36121 atagtgacgc gcctgccctt cgagggtttc gtcgtcacaa ataaaggtgc agtgcatggc
    36181 aaacggtggc agccagttca gtccgcagta gattgccgtc gcctgtagcg gctgcgacag
    36241 cacatcaaag cccggatgcg caccaatttc aaaatggctt tccccgccgc cggtcgtcac
    36301 cgcccacagc aaatgtttgc catgcagcgc cgtgccgcca tgaccgtaag cccagccgtg
    36361 ggagaaaact ttatcgatcc aaagtttgag gagcggagga atgctgtacc actgcatcgg
    36421 gtgctgccag acgatcagat cggcgcgaga cagcgcctcc tgctcggcgg caatatcgat
    36481 attgaagtca ggatagagtt gataaagaga gcgaatttcg acgccttcca gcgtccttgc
    36541 ctgttcaagc atccgtttat tcgcatggga atgatgcgga tacggatgcg cataaattat
    36601 aagaatcata ggtagcccgc gtgattacgc ctttgttttc ccacaaagtg tagtcatata
    36661 ttcacgcggc ttatagtcaa taatattgat tcagaaaaga gagaaatctg aacaatttac
    36721 cccattctct gcccgcggat cgtcatattg ctggtttgcg ccagtgacat ccctcgagtc
    36781 tccggggcaa acgctacgga aatcaacaag ccaaacagcg agatacccgc ccccattagc
    36841 atcgtgttac tgataccgta attattgata aagatcggca gtgcccaggt cgaaacaatg
    36901 gtgccaatac ggcttaagga cataatcacg cccacggcag aggcgcggat atctgtcggg
    36961 aagagttcat taggatagag ccactgcaaa ttacccggcc cgccagagaa aaaggcatac
    37021 accgcaaagg ccatcactac cagccagatc cccatatccg ggatcagccc caaaaccgcc
    37081 agcgccagcg tcatcatggc aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt
    37141 aaccacagca tcggcggaat acagccgagc ataaagaaca ggctaatcac cacgttcccc
    37201 agcgccgcgt ttttgccaac acccaatccc aacaaaccaa cgatttgcgg gccaaaggtg
    37261 taaatggcga acattgggat cacctggcag gtccagatgg cggcaacaaa cagaacaaaa
    37321 ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt gctgcggctg ctcttcatcg
    37381 aaagccaccg gttcgccaaa cagttttatc atcatctctt cgcactcttt tactcgccct
    37441 ttgcgtaata accagcgggg agattcaggc agttcgaatc gaccaatcaa aatcaacaaa
    37501 caggggatcg ccgcgctacc cagcatccag cgccagccgc cttccacatc ataaagccag
    37561 tagccgacca gatcggcgca ggtcgcgccg acataccaca tggcggcgat aaagctgatg
    37621 gaaaacgccc gctgacgggt actggagaac tcggtgatca ttgaggtggc gatgggataa
    37681 tctgcaccga tgacaatgcc gataagtacc cgcatcacca acagttcgac gggggatgaa
    37741 acaaacatcg tcgccaccga tatcacgccg atggcgatga tatcaatgag gaacattttg
    37801 cgccgtccga ctttatcgga aatatagcca aacagcgatg tgccaacgaa cagcccggcg
    37861 agcgttcccg cgcccaacaa gccaatccag tcagcgtcca gttttagcgc cggagtcagt
    37921 tgctccagcg ccacgccaat cattaccagt acataaccat ccagaaacgg accaccgctt
    37981 ccccacagca aaatgcggcg gtgaattgag gagaatttga gatcgtcaaa gtttctggac
    38041 ggttgcatgg gttattcctg tttttttgca atgagagtca gtacggcgtt acgtatcaaa
    38101 ccaacatcag ccgtaacgga actccacacc aaaggtgccg cgcgggtatt cccactgttc
    38161 cagcgccgac cccagcccca gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc
    38221 gaagcgcaca ctgccgtcat cctgcttctt gtacagacct gcggggcacg ctttcaccag
    38281 cagctccagg gcctgtttat cggcatcggc cttcacaaca atgtgcggat gctcttcatc
    38341 gacattgaat ttattgacgc ccagtttgac gtccacattg acgggagaag tcataaaacg
    38401 gtcactcctt tcatgccatc cttgatcaga ttgatgaagc ccactttctt gccgtggcgg
    38461 aggattttct tgcgcatcag ttccggcgca ctgccgtcaa tggtgaacag gtcacgcgcc
    38521 acgcccaccg ccagttccgg gtagccgcta aacatgcgtg ggttatcaag gaaggccggt
    38581 agtttctggt acatacgcat atcgcgcagc gggccactct caagatgctg acgatattcc
    38641 gcgagttttt gcttactgaa atcgtcgctt ttcatcgctg aaagcacggt ttttgctgca
    38701 gcttccccgg cggcaatcgc cagatccata ccgcgaatgg taaaaccgag gttcatacac
    38761 attccggcgg catcaccggc aatcaatacg ccgtcaccaa ccaactccgg cagcatgttg
    38821 atgcctgctt ccggcactac gtgagcggaa tattccacca gcttgccgcc cgcgatcagc
    38881 ggtgccactg ccggatgctg tttgaaatct tccagcattt gcggcaccga tttttttgcg
    38941 tcatgcagat gatgcagacc acaaaccagc cccagcgaca gggtattttc attggtataa
    39001 aggaagccgc cgcccatcag gccatcggtg ggtgatcccg caaacagaca agccgcgccc
    39061 tgattaccct gcaactgaaa acggtcttcg attaccgact tcggtaactc gatcagttcc
    39121 ttcacgccaa ccgccacatc cgtcggttta acgcgttttg ccatccccag cttttcggca
    39181 aggatggagt tcactccatc agcaaggatc accgttttcg cttcaatcac atcgccatcg
    39241 gcttctacac cgacgacttt gccatcgcgc tgtacgaggt tatcgacgcg gatcccggta
    39301 attaactgcg cgcccgcttc ttcggcctgc tccatcagcc aggcatcaaa tttactgcgc
    39361 aaaacggagt aagaacgctg ggatggcgag gtttcgtcac cattgcagta gtccatagtc
    39421 atcgctgact tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg ttctacgggg
    39481 gcggagtcgg cgaaaccagg aataatgtgt tccaggctgt gggcatagag acgcccgccg
    39541 gtgacgttct tggcacctgc ggaattgcca cgctcgataa ctaacacttg cgccccttcg
    39601 cgggcgagca ccagtgcggc aaccgaaccg gcaagcccag caccgacgat gatggcgtca
    39661 aagatatctt cggacataag agctccctgt cagcggcagg gccaaaatgc agccctgcca
    39721 gagtggatca acgcgctaaa gctgcggtca gcgccggaag gatcttcacg gcgtcaccaa
    39781 caatgccgta atccgcgtac tggaagatcg gcgcattttt atctttattg atggcgaaaa
    39841 tggtttgcga tgcattagca ccaaccatgt gctggatctg cccggagatc cccaccgcca
    39901 ggtacagttc aggtttcagc atcaggttgg agataccgac atagcgttcg tgctccatcc
    39961 atttttcgtt ttccgccacc ggacgagaac aggccaactc cgcacctatc gccttgcaaa
    40021 gctgttctgc cagcgcaatg ttctctttgc tgccaatacc gcgaccgacg ctgaccacca
    40081 gacgggcttt gtcgagatcg acgctgttgc tctggcgcgc ctgggttgcc gtgcgggtaa
    40141 tcgccacagc cggagcctgc cactccacgg tgtgcgtttc gccagtgcgt gacgcgtctg
    40201 gctgagccgc atcgaacgtg ccgctgctga tggtcagtac cgcatacggc gtggcaatgc
    40261 gttcttcgcc aatcgccaga ccaccgtaaa ccatgtgttt cactgtcgct ttaccgtcct
    40321 gtacgctgac ggtgctggca tcgttagaca ccgccgcttt aaggcgataa cccagttttg
    40381 ccgccagtaa tttgccgcga cgggtgtttg gcagcagcac caggccgtct gcgccgtgct
    40441 ggcgaatagt gtcagccatg acaccggcgt aatcttcgat catccgatcg tccggtttgc
    40501 cgtttaattt ccagacatga ttagcgccga gctggattgc ctgtacgccg tcggcatcat
    40561 tgaggacaaa ggtgttgatt tgattagcta aagcctgcgc accgttcatc agttccggca
    40621 gacgagaagg ggtatcgctg aatacccaga cttgagaaaa cgtgttcata gcatcccctg
    40681 taattaaatg actttgcgaa gattttcagc aaatgcggcg atctgttctt cgccgtcgcc
    40741 ttcaatcacg atgcgctgac gttcgcgctg tttcggcgca gcaacctgtt gttcagacca
    40801 ggcgtccacg gcgttaaaac cgatatccgc cgccgaccat acctggactg gctttttcgc
    40861 cgcgccgaga atggctttca tcgaaggaat ttgtggggag ttgatatcag tggaaacagc
    40921 aacaaccgca ggcagcggaa tgcttaaggt ttcggtttca tcttccagtt cgcgctcaac
    40981 ggtgagggta tctgcagtca gggagataat tttgctgacg ccgttaactg ccggaatatt
    41041 gaggatttcg cccaccagca gaccaacctg ctgggcataa aggtcggaag aaccatcgcc
    41101 acagaggatc aaatcaaagc ctgctttctg ggcggctgca gccagtgcgc tcgccgtttg
    41161 ttgcggcagt gcctgctcga actggtcatc aatcaccaca atcagttcat ccgggccgcg
    41221 cgatagcaca tctttacgcc ctttggcgtt ggtcagggct ttaccgccca cacttaaggc
    41281 cgtcacctgc gcctctgctg cctgttgctt tagctggcaa gccgcttcaa tagcgttcag
    41341 atcgtattgg cttattttgg catcggcttt gctgaagtct aatgaaccat cagcattatt
    41401 gaccgcaata tcctgttcat caggcacgca cttatagcaa gtaataatct tcattgcatc
    41461 tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg ttaattacag atatcacgcc
    41521 ctttaatttg gaaataaaac tttaatcacc aatattgaaa atgtcacacg cagacaaatc
    41581 caactttcaa tattgttaag ttcctcacca atattgaaaa catcgcgtag caaaaagaaa
    41641 ttttcaatat tgctttatgg atcaccaata ttgaaagtca gatgctattt ttttactcac
    41701 aacacaacat aacaaactga ttaataatca aaattaacga gaaaagcgcc ctctattcac
    41761 ccgaaacaaa aatgtgataa caatcacaga atgcagctta ttgaataccc attatgagtt
    41821 agccattaac gcgtccacga ggttaataat aattatatta aatgttaaca aaaataaaac
    41881 aaacgggaac gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa
    41941 agagaaaaac gggaatagaa ccgaaggttt tctttccgcc gttaataatc gtcggcatac
    42001 tttgttggct tacagtcaga gatctggatg cagcgaatgt cgttattaat gctgtattca
    42061 gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg
    42121 gttggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccacctg
    42181 aatttagtac cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac
    42241 tgttctgggg atcgattgag atctactact acatctccac cccgccgttt ggcttagaac
    42301 cgaactcgac aggggcgaaa gagttggggc tggcttacag cttgttccac tggggacctc
    42361 tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca
    42421 aaatggaagt gattcgcccc agctcgacac tggtgccgct ggtaggtgaa aaacacgcca
    42481 aagggttgtt cggcactatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg
    42541 gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca
    42601 ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca
    42661 tttgcgtcgc ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc
    42721 tgagcttcct gatgctgggt tgggtgttca ttgtcagcgg tgccagcttc atcatgaact
    42781 acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg
    42841 atcccatcgc taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg
    42901 tgatttatgc tatccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc
    42961 gtgaactgtg cttcggcatg gtgctggggc tgacagcgtc aacctggatc ctgtggactg
    43021 tactcggtag taacactctg ctgttgatag ataaaaacat catcaacatt ccaaatctga
    43081 tcgaacagta cggtgtggcg cgcgccatca ttgaaacctg ggccgctctg ccactcagca
    43141 ccgccaccat gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg
    43201 cctgctctta taccctggcg atgtccactt gccgcgaagt acgcgatggt gaagaaccac
    43261 ctctgctggt gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttctgctgg
    43321 cgctcggcgg cctgaaaccg attcaaaccg ccattatcgc cggaggatgc ccgctgttct
    43381 tcgtcaacat tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt
    43441 aattaacccc caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg
    43501 aactgtttgt cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg
    43561 ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg
    43621 gtatcgacag tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc
    43681 tcgccgccgt gtggatggag ctgggacgtc tgggggcacc aacctatgtg ctgtaccagt
    43741 tgccgggcgg gttcaacacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta
    43801 tggctttccg cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg
    43861 gctccgacgt gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta
    43921 atggtagtaa gtgttttatt accagcagcg cctacacccc gtacatcgtg gtgatggcgc
    43981 gcgacggggc ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac
    44041 cgggcatcaa agtgaccaaa cttgaaaagc tcggtctgcg tatggatagc tgctgtgaaa
    44101 tcacctttga cgacgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct
    44161 ttaaccgcgt caaagaagag ttcgaccatg aacgtttcct ggtagccctc accaactacg
    44221 gtacggcgat gtgcgccttt gaagatgcgg cgcgctacgc caatcagcgc gtgcagtttg
    44281 gcgaggctat tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat
    44341 taaactccat gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca
    44401 cctctggcga tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagttgtgg
    44461 atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct
    44521 tctggcgtga tctgcgtgta gaccgcgtct ccgggggatc tgacgaaatg cagatcctga
    44581 cgctgggtcg cgcggtgctg aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc
    44641 taa
//
LOCUS       DAZFJF010000023        44292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFJF010000023 DAZFJF010000000
VERSION     DAZFJF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.023"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..420
                     /locus_tag="ACHKAU_001448"
     CDS             <1..420
                     /locus_tag="ACHKAU_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO5530547.1"
                     /translation="GLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLN
                     DDQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRA
                     LAAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            435..1292
                     /gene="menB"
                     /locus_tag="ACHKAU_001449"
     CDS             435..1292
                     /gene="menB"
                     /locus_tag="ACHKAU_001449"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO5530548.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1292..2254
                     /gene="menC"
                     /locus_tag="ACHKAU_001450"
     CDS             1292..2254
                     /gene="menC"
                     /locus_tag="ACHKAU_001450"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO5530549.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2251..3606
                     /gene="menE"
                     /locus_tag="ACHKAU_001451"
     CDS             2251..3606
                     /gene="menE"
                     /locus_tag="ACHKAU_001451"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO5530550.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3716..3982
                     /gene="pmrD"
                     /locus_tag="ACHKAU_001452"
     CDS             3716..3982
                     /gene="pmrD"
                     /locus_tag="ACHKAU_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO5530551.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(3976..4362)
                     /gene="arnF"
                     /locus_tag="ACHKAU_001453"
     CDS             complement(3976..4362)
                     /gene="arnF"
                     /locus_tag="ACHKAU_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO5530552.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4362..4697)
                     /gene="arnE"
                     /locus_tag="ACHKAU_001454"
     CDS             complement(4362..4697)
                     /gene="arnE"
                     /locus_tag="ACHKAU_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO5530553.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4694..6346)
                     /gene="arnT"
                     /locus_tag="ACHKAU_001455"
     CDS             complement(4694..6346)
                     /gene="arnT"
                     /locus_tag="ACHKAU_001455"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO5530554.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6346..7236)
                     /gene="arnD"
                     /locus_tag="ACHKAU_001456"
     CDS             complement(6346..7236)
                     /gene="arnD"
                     /locus_tag="ACHKAU_001456"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO5530555.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7233..9215)
                     /gene="arnA"
                     /locus_tag="ACHKAU_001457"
     CDS             complement(7233..9215)
                     /gene="arnA"
                     /locus_tag="ACHKAU_001457"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO5530556.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9215..10183)
                     /gene="arnC"
                     /locus_tag="ACHKAU_001458"
     CDS             complement(9215..10183)
                     /gene="arnC"
                     /locus_tag="ACHKAU_001458"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO5530557.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10187..11326)
                     /gene="arnB"
                     /locus_tag="ACHKAU_001459"
     CDS             complement(10187..11326)
                     /gene="arnB"
                     /locus_tag="ACHKAU_001459"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO5530558.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            11634..12236
                     /gene="ais"
                     /locus_tag="ACHKAU_001460"
     CDS             11634..12236
                     /gene="ais"
                     /locus_tag="ACHKAU_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO5530559.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(12275..12700)
                     /gene="nudI"
                     /locus_tag="ACHKAU_001461"
     CDS             complement(12275..12700)
                     /gene="nudI"
                     /locus_tag="ACHKAU_001461"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO5530560.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            12979..13521
                     /gene="yfaZ"
                     /locus_tag="ACHKAU_001462"
     CDS             12979..13521
                     /gene="yfaZ"
                     /locus_tag="ACHKAU_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO5530561.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            13621..14823
                     /gene="yfaY"
                     /locus_tag="ACHKAU_001463"
     CDS             13621..14823
                     /gene="yfaY"
                     /locus_tag="ACHKAU_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO5530562.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            14887..15348
                     /locus_tag="ACHKAU_001464"
                     /pseudo
     CDS             14887..15348
                     /locus_tag="ACHKAU_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(15389..16291)
                     /gene="rpnE"
                     /locus_tag="ACHKAU_001465"
     CDS             complement(15389..16291)
                     /gene="rpnE"
                     /locus_tag="ACHKAU_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO5530563.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(16485..17675)
                     /gene="glpC"
                     /locus_tag="ACHKAU_001466"
     CDS             complement(16485..17675)
                     /gene="glpC"
                     /locus_tag="ACHKAU_001466"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO5530564.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(17672..18931)
                     /gene="glpB"
                     /locus_tag="ACHKAU_001467"
     CDS             complement(17672..18931)
                     /gene="glpB"
                     /locus_tag="ACHKAU_001467"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO5530565.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVNTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(18921..20549)
                     /gene="glpA"
                     /locus_tag="ACHKAU_001468"
     CDS             complement(18921..20549)
                     /gene="glpA"
                     /locus_tag="ACHKAU_001468"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO5530566.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            20822..22180
                     /gene="glpT"
                     /locus_tag="ACHKAU_001469"
     CDS             20822..22180
                     /gene="glpT"
                     /locus_tag="ACHKAU_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO5530567.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            22185..23261
                     /gene="glpQ"
                     /locus_tag="ACHKAU_001470"
     CDS             22185..23261
                     /gene="glpQ"
                     /locus_tag="ACHKAU_001470"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5530568.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(23303..23542)
                     /locus_tag="ACHKAU_001471"
                     /pseudo
     CDS             complement(23303..23542)
                     /locus_tag="ACHKAU_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            23540..23644
                     /locus_tag="ACHKAU_001472"
                     /pseudo
     CDS             23540..23644
                     /locus_tag="ACHKAU_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23724..24374
                     /gene="inaA"
                     /locus_tag="ACHKAU_001473"
     CDS             23724..24374
                     /gene="inaA"
                     /locus_tag="ACHKAU_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO5530569.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(24428..24682)
                     /gene="yfaE"
                     /locus_tag="ACHKAU_001474"
     CDS             complement(24428..24682)
                     /gene="yfaE"
                     /locus_tag="ACHKAU_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO5530570.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(24682..25812)
                     /gene="nrdB"
                     /locus_tag="ACHKAU_001475"
     CDS             complement(24682..25812)
                     /gene="nrdB"
                     /locus_tag="ACHKAU_001475"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5530571.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(25901..28186)
                     /gene="nrdA"
                     /locus_tag="ACHKAU_001476"
     CDS             complement(25901..28186)
                     /gene="nrdA"
                     /locus_tag="ACHKAU_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5530572.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            28314..28457
                     /gene="ypaB"
                     /locus_tag="ACHKAU_001477"
     CDS             28314..28457
                     /gene="ypaB"
                     /locus_tag="ACHKAU_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO5530573.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            28882..32616
                     /gene="yfaL"
                     /locus_tag="ACHKAU_001478"
     CDS             28882..32616
                     /gene="yfaL"
                     /locus_tag="ACHKAU_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO5530574.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEDYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(32744..33466)
                     /gene="ubiG"
                     /locus_tag="ACHKAU_001479"
     CDS             complement(32744..33466)
                     /gene="ubiG"
                     /locus_tag="ACHKAU_001479"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO5530575.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            33613..36240
                     /gene="gyrA"
                     /locus_tag="ACHKAU_001480"
     CDS             33613..36240
                     /gene="gyrA"
                     /locus_tag="ACHKAU_001480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO5530576.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            36389..38077
                     /gene="yfaA"
                     /locus_tag="ACHKAU_001481"
     CDS             36389..38077
                     /gene="yfaA"
                     /locus_tag="ACHKAU_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO5530577.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            38074..38679
                     /locus_tag="ACHKAU_001482"
     CDS             38074..38679
                     /locus_tag="ACHKAU_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFO5530578.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKISLIINMLMCAGHY"
     gene            complement(38694..39764)
                     /locus_tag="ACHKAU_001483"
     CDS             complement(38694..39764)
                     /locus_tag="ACHKAU_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5530579.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEIS"
     gene            complement(39742..39960)
                     /locus_tag="ACHKAU_001484"
     CDS             complement(39742..39960)
                     /locus_tag="ACHKAU_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5530580.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTSSLLKRIRNGKKAKS"
     gene            complement(40066..40410)
                     /locus_tag="ACHKAU_001485"
     CDS             complement(40066..40410)
                     /locus_tag="ACHKAU_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530581.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(40529..40771)
                     /locus_tag="ACHKAU_001486"
     CDS             complement(40529..40771)
                     /locus_tag="ACHKAU_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5530582.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQRR"
     gene            complement(40846..41196)
                     /locus_tag="ACHKAU_001487"
     CDS             complement(40846..41196)
                     /locus_tag="ACHKAU_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5530583.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            complement(41493..41798)
                     /locus_tag="ACHKAU_001488"
                     /pseudo
     CDS             complement(41493..41798)
                     /locus_tag="ACHKAU_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(41795..42016)
                     /locus_tag="ACHKAU_001489"
     CDS             complement(41795..42016)
                     /locus_tag="ACHKAU_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5530584.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(42115..42330)
                     /locus_tag="ACHKAU_001490"
     CDS             complement(42115..42330)
                     /locus_tag="ACHKAU_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5530585.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(42407..42598)
                     /locus_tag="ACHKAU_001491"
     CDS             complement(42407..42598)
                     /locus_tag="ACHKAU_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5530586.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            complement(42571..42753)
                     /locus_tag="ACHKAU_001492"
     CDS             complement(42571..42753)
                     /locus_tag="ACHKAU_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO5530587.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            complement(42753..43430)
                     /locus_tag="ACHKAU_001493"
     CDS             complement(42753..43430)
                     /locus_tag="ACHKAU_001493"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5530588.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            complement(43427..44212)
                     /gene="bet"
                     /locus_tag="ACHKAU_001494"
     CDS             complement(43427..44212)
                     /gene="bet"
                     /locus_tag="ACHKAU_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5530589.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(44218..>44292)
                     /locus_tag="ACHKAU_001495"
     CDS             complement(44218..>44292)
                     /locus_tag="ACHKAU_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5530590.1"
                     /translation="RMAEHIRYMVETIAHHQVDIDSEV"
BASE COUNT        10621 a        11015 c        11683 g        10973 t
ORIGIN      
        1 gggctgcaaa atgctgaaca acgtgcggaa cgtcagcgtt ccgatcgcca atgggcgcag
       61 cgtttttgca cagaaccgtt aacggcggta tttgccgact ggtatcaaca gcctgttttt
      121 gcctcgctca atgacgatca acgccgggag ctggtggcgc tgcgcagcaa caataatggc
      181 gcaacccttg ccgccatgct ggaggcgact tctctcgccg tccagcctga tttacgtgct
      241 aaccttagcg cccgcacatt tgcgttttat tatttatgtg gtgaacgtga cagcaaattc
      301 cgcgccctgg cggcggaact ggctgccgac tgccatgtca ttcctcgcgc cggacataac
      361 gcgcatcggg aaaatcccgc tggcgtaatc gcaagtctgg cgcagatctt gcgtttctga
      421 ctaaaggaca caatatgatt tatcctgatg aagcaatgct ttacgcaccg gttgaatggc
      481 acgactgctc cgaaggtttc gaggacattc gttatgaaaa atccaccgac ggtatcgcaa
      541 aaatcaccat taatcgtccg caggtgcgca atgccttccg tcctctgacg gtaaaagaga
      601 tgatccaggc actggcagat gcgcgttatg acgacaatat cggcgtgatc attctgactg
      661 gcgcaggcga taaagcgttc tgctccggtg gtgaccagaa agtgcgtggt gattacggcg
      721 gctataaaga tgattccggc gtacatcact tgaacgtgct ggacttccag cgtcagatcc
      781 gtacctgtcc gaaaccggtt gtcgcgatgg tggctggcta ctccatcggc ggtggtcacg
      841 ttctgcacat gatgtgcgac ctgactatcg cagcagataa tgccatcttc ggtcagactg
      901 gcccgaaagt cggttccttc gacggcggct ggggcgcttc ctacatggct cgtatcgtcg
      961 ggcagaaaaa agcgcgagaa atctggttcc tgtgccgtca gtacgacgca aaacaggcgc
     1021 tggatatggg ccttgtcaac accgtggtac cgctggcgga tctggaaaaa gaaaccgtcc
     1081 gttggtgccg cgaaatgctg caaaacagcc cgatggcgct gcgctgcctg aaagctgcgc
     1141 tgaacgccga ctgtgacggg caggcggggc tgcaggagct ggcgggcaac gccaccatgc
     1201 tgttctacat gacggaagaa ggtcaggaag gtcgcaacgc tttcaaccag aaacgtcagc
     1261 ctgatttcag caaatttaaa cggaatccgt aatgcgtagc gcgcaggtat accgctggca
     1321 gatccccatg gacgcggggg tggttctgcg cgacaggcgg ttaaaaaccc gcgacgggct
     1381 gtacgtttgc ctgcgtgagg gcgagcgcga agggtggggg gagatctccc cactgccggg
     1441 cttcagccag gaaacctggg aagaggcgca aagcgtgctg cttgcctggg taaataactg
     1501 gctggcaggc gattgcgagc taccgcagat gccttccgtt gcctttggcg taagctgtgc
     1561 attggcggag ctggcagata cgctcccgca ggcggccaat taccgtgcgg caccactgtg
     1621 taatggcgat cctgacgatc tgatcctcaa acttgctgat atgccaggcg agaaagtggc
     1681 gaaggtcaaa gtgggattgt acgaagccgt gcgcgacggc atggtggtta atctgttgct
     1741 ggaggcaatt ccggatctgc atttgcgtct tgacgcaaat cgcgcctgga caccgctgaa
     1801 aggccagcag tttgccaaat atgttaaccc ggattatcgc caccgcatcg cgtttctcga
     1861 agagccgtgc aaaacccgcg atgattcgcg agcgtttgcc cgtgaaaccg gcattgccat
     1921 tgcctgggat gaaagcttgc gcgagccgga ttttgccttt gtggctgaag agggcgtgcg
     1981 cgcggtagtt atcaaaccca cgctcacggg cagtctggag aaagtacgcg agcaggtaca
     2041 ggcggcgcac gcgctggggc tgacggcggt gatcagttct tccattgaat cgagcttagg
     2101 cttaacgcaa ctggcgcgga ttgccgcctg gttaacgccg gacaccattc cagggctgga
     2161 cacgctggat ctgatgcagg cgcagcaggt acgtcgctgg ccgggtagca cgctgcctgt
     2221 cgtggaagtt gatgcactgg agcggttgtt atgatcttct ctgactggcc gtggcgtcac
     2281 tggcggcaag tgcggggaga agccatcgcc ttacgtctta atgacgagca acttaactgg
     2341 cgcgagcttt gtgctcgcgt cgatgaatta gcctcaggct ttgcggcgca gggcgttgtt
     2401 gagggcagcg gcgtgatgtt gcgggcgtgg aatacgccgc aaacgctgct cgcctggctg
     2461 gcgttactgc aatgcggggc gcgggtgttg cccgtgaacc ctcagctgcc gcaaccgttg
     2521 cttgaagaat tgctgcccaa tctgacgctg caatttgcgc tggtgccgga tggggaaaac
     2581 acgtttccgg cgttaacgtc gctgcacatt cagcgggttg aaggcgcaca tgccgctacg
     2641 tggcagccga cgcgtctgtg ctcaatgacg ttgacctcgg gttctaccgg gttgccgaaa
     2701 gccgctgtcc atacttatca ggcccatctt gccagtgccg aaggggtgtt atcgttgatt
     2761 ccgtttggcg atcacgatga ctggttgctc tctttaccgc tgtttcacgt ctccggtcag
     2821 ggaattatgt ggcgctggtt atacgctggt gcgcggatga cggtgcgtga taaacagcca
     2881 ttggagcaaa tgctggcagg ctgtactcat gcttcactgg tgccaacaca actctggcgt
     2941 ttgctggtta accgcagttc cgtttccctg aaagcggtgt tacttggcgg cgcggctatc
     3001 ccggtcgagt tgacggaaca ggcgcgcgag caggggattc gttgcttttg cggctatggt
     3061 ctgaccgagt ttgcctccac ggtgtgtgcg aaagaagccg acggcctggc agacgttggt
     3121 tcgccgctgc cgggtcggga agtgaaaatc gttaataatg aagtgtggct gcgggctgcc
     3181 agtatggcag aaggttactg gcgtaacggg caactggttt cactggttaa tgacgaaggc
     3241 tggtacgcta cgcgcgatcg cggtgagatg cataatggca agctgaccat tgtcggacgt
     3301 ttagacaatc tattcttcag tggcggagag ggtattcagc cggaagaagt cgagcgtgtt
     3361 attgctgcac atcctgcggt tttgcaggtg tttatcgttc ccgttgccga caaggagttt
     3421 ggtcatcggc cggtagcggt ggtggagtat gaccagcaaa ccgttgatct tggcgaatgg
     3481 gtgaaagata agctggctcg ttttcaacaa ccggtgcgct ggctgacttt accgccggag
     3541 ctgaaaaacg gcggtattaa aatttcacgt caggcgctaa aagagtgggt gcaacgtcag
     3601 caataattaa ccctccgttt cacttctcat ttctgccatt ccattgccgt tgataaagag
     3661 tttatctgtt aatctgtaaa ttgatgtgaa aactcttagc aaacaggata atgcaatgga
     3721 atggctggtc aaaaaatcgt gctgcaataa acaagataac agacatgtac ttatgctctg
     3781 cgatgctggc ggcgcgataa aaatgatcgc cgaagtgaag agtgacttcg ccgtgaaagt
     3841 gggggattta ctctcgcctt tgcagaatgc gctttattgt attaatcgtg aaaagctgca
     3901 tacggtgaaa gtcctctcgg caagcagtta tagccccgat gagtgggaac ggcagtgtac
     3961 agcggcaggg aaaactcagt aacgttgttt tgtcgtgggc aggaaaatca gcatcaaccc
     4021 gctcataata caggctactc ccagtagtgc tttcagcgaa aaagtccctt cccagccggg
     4081 aagtaccatt gaagcaatcc ataccagcac ataactcata ctgagcaagg cataggcttt
     4141 gcttagcgca agtttatgta gcgttttata ccaacaaaaa acggaaagca gataacccag
     4201 cagcccgagt aacagtatcc gggcatccag gccaaaagct aacagagccg cgataaaatc
     4261 ccataagtgc gtcatcggcg gcagatgact tgccgcaaaa cccaggctta attgcgcaac
     4321 tgaggcaata atgacgctga ataatcccca tatcaggccc attacaccgt actcccgagg
     4381 atcacaatgc caccaataat gaacgccacc ccacaccagt gacgcggcga taccggttcg
     4441 tgccacagtt ttactgcagc cagcgtcacc cagacaaaat tcagacttaa catcgggtag
     4501 gcaatgccta ccggtacgtt ctgcaagacc agcagccaga gcaccatggc aagaccaaga
     4561 caagccagcg ccagtcccag ccacagcacg atatgtttgc gccgtttgtt tatcgccaca
     4621 aagcaggttg cctgtttctg acacaactgc ccggcaacgc taagcaagct ggcaaagact
     4681 aatgttagcc agatcatttg ggacgatact gaatcagcac cagacgctcc tgacgatcga
     4741 tggcatctgc gggcggaatg gcgagactgt tgatatcttc atcgcggtca accgaaagca
     4801 cgagagtaat aatcccctcc tgacgatgtt gattaagcca gtttgcgaac tcatcaccgc
     4861 tgacaaatct ccctttcgca tccggataat taaggccgta tttcaactca cctgtctggc
     4921 gatacatgat gatgtcatcg cgttgcaggc tccatgccag acctgcggca acaccgacgc
     4981 tatcagtaag aatatagcgg cttggctgaa gtgattcttg tgtcatctcg acaaaaaatt
     5041 gcggatgttt tccttccata actctgtcag gaattgaaaa tcctaccagc aacgccagcc
     5101 ccagcgggca aagcgcggca aaaggccagg tcttttcgac gtttgttaag gtgtaccagc
     5161 cgaaaaatgc ccatagcgaa aaaatcgacc aggcacaaaa gactttatag ctctcgaagg
     5221 tttgccacac cggcgtgttc attggtcccc acggggagac cacaaatgtg gcaataatgc
     5281 cagtgacgcc aaaagcgatg ttaatccagc cattaatccg cagcgccaga ggattatttt
     5341 ttgctgccag caaagcgtaa tgcgccatca gcattgccag agatgcaaag caggaaagaa
     5401 tataggtggg caatttacct ttagcgacgg agaaaaacag cagcggcatt atcgtccagc
     5461 tcaacaaata gacggttgcg gaatgcttgc ggtttttcca gcctgtgtac agtgcaccgg
     5521 ggagtaatcc cagccacggc aggctaccgg caatgatgac cggcacgtag taccagaacg
     5581 gagctctatg ttgggcatca tccagtgcaa agcgttgaat atgctcaacc cagaaaaaat
     5641 agtgccagaa gtcaggctcc cgctgcgcta tcgccagtcc ccaagggaga accgtcagta
     5701 cgcaactgat aaccgccagc cagccgtaaa taaagagatc tttccagcgt ttttgcgtcg
     5761 ctacccatgg cagcacgctt aataccggca cggcaagggc gagaaaacct ttggtcatca
     5821 cccccatgcc gcaggtgatt cccagcagta aaaatcctgc gcttttgcct ttccacgtct
     5881 gtgcctgcat tgccagccag aagctgcaca ttcccgccac cagccagaat gcaataaacg
     5941 gatcgagcac ggcataagtg ccgatggcat agacaataaa caatgagaga taaattactg
     6001 tggcgagtag agccagacgt ttatcgcgcc ataagcgcag agtaaaccag gtcaccagcg
     6061 cggcagttaa cagggtcgca aagataacgc ctgcccgcac accaaagtta ttcgcgccaa
     6121 atagccattg cccaatgctg ttaatccagt atccggcaat gggtttttcg aaataacgta
     6181 gccctaacag atggggcaca atccagtcgc cggatgccag catttctcga ctgatttccg
     6241 cataacgcgt ttcatcgggt tgccagagca gacgcgtgct gatcggtaac aggtaatagc
     6301 aggcaataaa cgcgaagagg ccgataaggt aacgtaccga tttcatcagc gactacccgc
     6361 aatttgttgg caacccagcc agccttcacg tccggcaata tttccgcgaa caacttgtcc
     6421 gagcggcaac gtctctgata acagttcgct taaagggcaa aatgtcactc cttcctgagc
     6481 tgcgcgtttg aggagatcca caaaattatg ctgataagcg cagccttcaa cttctgcatg
     6541 aatggtataa accggcgtgc ctttatctcg caggatgcgg ttgagtaacc aaccgttaaa
     6601 atcttctgct ttcacatccc gaccaatcac ttcatcccag gtgggtaagg tcaccggaat
     6661 ttgcgcagtg ccagggtttc ctgattcgag caatggacgg aacggcatgg ccccacgaca
     6721 atcgctgttg tagcgcaaat ggaacgcttc ttttgcttcg atcaccttct ggtcggcacg
     6781 ccagcccgca gcggcagaac aggttaccgg ttgaccgata atctcttcca gagtgcgaag
     6841 accgcgtgca atatcgtcga tcattgtttg ccgatcccag ttaccgctac gggcttgcca
     6901 ggcatggtga tcccaggcgt gcaggccgac ttcgtgatgt ttagccgctt cacgaatgat
     6961 atcggcattg gcatgaccaa tctctttacc tggccaggcc gtacctgcca gtaaaatatc
     7021 ccagccataa agcgatgccg catttgagcg cagcatcttc cacaaaaact gtggcttcac
     7081 cagtcgccag agatggcggc ccatattgtc cgggccgacg ctgaaaaaaa tgctggcctg
     7141 aatattatgc ttactcaaga tttccagcag acgcggcacg ccttcacggg tgccacgaaa
     7201 ggtatcgaca tcaatgcgta agcctacttt ggtcatgatg gtttatccgt aagatcaacg
     7261 gtgcgcagga agaaatccag cgtttcgtcg atggtttcct gcatatcaat tttcggctcc
     7321 cagtccaggc agcggtgggc attgcggatg ctcggtttac gatgctctac gtcctgatat
     7381 cctttgccgt agtagctgct actttcgaca acgcgaaagc ccgcaaacgg tgggaaatga
     7441 tggcgcagcg gatgtttttc gaagctcgcc agcagcatct cgcccagttc ctcaatgctc
     7501 gcttcgttct caggattgcc aatgttgata atttcaccgt cgcagcgatt tcccgcattt
     7561 tcgataatgc ggtataacgc ctcgatacca tcgcgaatat cagtaaagca gcgtttttgt
     7621 tttccgccat caatcagctt aatcggtgaa ccttctacca gattgagaat gagttgcgta
     7681 atagcgcggg agctgccgat tcgtgccgca ttaaggttat ccagtcgcgg tcccatccag
     7741 ttaaacgggc ggaagagggt gaactgtaaa ccctcttttt cgccataggc ccagatcacc
     7801 cgatcaagta attgttttga taccgaataa atccagcgtg gtttattcac cgggccgacg
     7861 attaaattag aatggtcctc atcgaagtat ttatcgctac acatcccata aacttctgaa
     7921 gttgacggga agatgattcg cttacggtac ttcacgcagt agcggataat gcgcagattc
     7981 tcttcaaaat cgagttcaaa tacgcgcagc gggttgcggg tatattcaat cggcgtggct
     8041 atcgccacca gcggcaagac gacatcacat tttttgacgt gatactcaat ccattcggaa
     8101 tgaatactga tatcgccttc aacaaagtga aaatgcggat gattcagaaa acggcttatc
     8161 gcatcactgc caatatccag accgtaaact tcataatgat cttcgcgcag caggcgttct
     8221 gtcagatggt tgccaataaa gccattcacc ccgaggatga gtacccgggt acggcgtcgg
     8281 gcggtgcagg caggctggct attcaagcgt gaaccttgca ccaggcccag cgtctgcgct
     8341 aattgcgagc cctgcatagt aatgccgtcg cccgcctgtc cggtgacgat ttccagcgcg
     8401 ccatcgccac aggcaatcag cagtggcgca acagaaatca cgctccccgg ctgtgctttg
     8461 ctggcatgag gatgaacacg cgacgaccag acggtgaatt tctgattgcc aacatagctg
     8521 aaggcacccg gccacggatc ggcaacggca cgtaccatgt tgtgcagtac ggatgccggt
     8581 ttatgccatt caaggaaact gtcatccggc gttctgcgac caaaacaggt ggcttcgttt
     8641 tcgcgctggg cgatttccag aatattaccg tgtttaatgg cgggtaatgt ctgttccagt
     8701 agctggcgcg cggcatggca caatttatga tgcagcgtaa tagcgatatc gtctggcgca
     8761 atggcaatgc gcagttgcgc cacaatggcc ccggcatcgg cacgtttcac cattcggtgc
     8821 aatgtaacgc cagtttccgt ttcaccgttg accagcaccc agttcagcgg cgcgcgacca
     8881 cgatattttg gtaacagcga accatgcaga ttaaatgcac ctgcgggagc gagctgcaaa
     8941 atttcgtcgc aaataagatg gcgataataa aaagagaaaa tcacatctgg cgacagttgg
     9001 gcaatgcgtt ccacccacag cggatgatta acgtcatccg gcgcataaac cggaatgcct
     9061 ctttccgctg ccagacgagc caccgaacca taaaaggctt tttcaccggg attatcggta
     9121 tgggtaaaaa tggcgctaat ttcgtaaccg gcagccagca gagcttcaat accgaggcat
     9181 cccatatcgt ggtaggcaaa aacgacggtt ttcattattc attttccttg ctggatggac
     9241 ggataacttg ctgaacaaaa tagcgggggc gggcgcggac atcggtgtag atcctgccga
     9301 tatattcacc gagtaatccc atgccgataa actgagcgcc aataaaagta aacagcacgg
     9361 caaatagcat aaagacgcct tctgccgccc attgtggtcc gaaggtcagg cgtaaaatca
     9421 ccagcagcac cgcaatgcta aaacctccaa tcgcaataat gctgccgagc agactcagca
     9481 tacgtagcgg cgttgtggta aggcaggtca ccaggtcgta catcaaatta atcaggcgca
     9541 taaaactgta tttggattca ccaaactcac gctcggaatg atgtactgga atttcaatgg
     9601 cacggcgggc gaagatattc gccagaatcg ggataaaggt gctacgttca tggcagtgca
     9661 acatcgcatc gacaatatga cggcgatagg cgcgcagcat acaaccgtag tcacccatcg
     9721 ctttgccggt ggtgcgctga ataagccggt taatcatctt cgaagctgtt ttacgaaacc
     9781 agctgtcctg gcggttctgg cgtacagtcc ctaccacgtc gtaaccttca tcggcttttg
     9841 ccaccaggcg ggggatttct tctggcggat tctggagatc ggcatcaagg gtaataatta
     9901 agtcgccggt aacgtgactg aatcccgcca taatcgctga atgttgcccg taattgcggt
     9961 taagcaaaat agacacaata tggctgttct ccgcttgtga ggcttcgacc agcatatgcg
    10021 cggaattatc gctactgccg tcatcaatca gcaggatctc atactctttc cccaacgatt
    10081 cacaggctgt ggtggtgcgc ctgattaatt ccggtaagct ttcctgctcg ttataaacgg
    10141 gaataaccac cgagactttc ttaacagggt ggatttcaaa catggcttat tgtcctgcga
    10201 gttgctgaag ggctgtgatg acacggtcgg catcggcggt agtcatatcc gggaacagcg
    10261 gcaaagaaca gatgcgttcg ctattccatt cggtattcgg taacgacagc gtgggaaaac
    10321 gctcgcgata atatttttgt gtgtgagcgg cgcggaaatg taacccggta ccaatgcctc
    10381 tttcttttaa cgcttccatc aacgcatcgc gactgatacc acaacgttgt tcatcgacac
    10441 gaataataaa cagatgccag gcgtgaacgt gcggccaggc gggaaggctt aatggctgaa
    10501 aggggagagc tgccagtgct tgctgatatt gctgggcaat ttcgcgccga cgggtgttga
    10561 ggtgctctaa ttttgctaac tgtgtcaggg caattgcggc gttaatatcg gtcagattgt
    10621 acttatagcc cggtgttaag acttcagcct gcggtgcacg gccccaggtt tgtctgtcat
    10681 aggcatcgac acccagaccg tgaaatttca gcatccgtag ctggcgggca aggttttcat
    10741 tatcagttac aatcaggcca ccttccgcac aggtaatatt tttaatggca tgaaatgaaa
    10801 aaatagtggt accttttgcg ccaatatgtc gccctttgta atacgtaccg acggcatggg
    10861 cagcatcttc gataactgcg atgccgtaac gttcgccaat ggcgcgaatg gcgtcaatat
    10921 ctgctggcgc accggcataa tgcaccggaa tgatggcttt agtgcgtggc gttatggctg
    10981 actcgatcgc ttcaggcgtg accatcggcg tatcgcggtc gacatccacc attaccggcg
    11041 ttgcacccaa caaggaaatc atattgaggg ttgaaaccca ggtcagggaa ggcgtaatca
    11101 cttcatcgcc cttgccaatt tccaacgcca ttagcgtgat atgcattccg gcggtggctg
    11161 aactgaccgc gatggcatgc tgatttcccg tcaactggca aaaggcttgc tcaagcgcct
    11221 gattcttcgg accggttgtg atccaaccgg attcgagaac ctctttaact gcagcgagtt
    11281 cctccacgcc cattgctggt cgcgaaaaag gcaaaaattc tgacattgct tttccttccg
    11341 ccattgaata cctgtccact tatatttgct ataaagagtg ttgtgtatat tttgccattt
    11401 ggagcgaaat tttaaggata gaatattaac ttaaccttaa gaaactaata ttagacgtaa
    11461 atattgaaat ttttatattt tttcttattt aggctttgca tttggcaaaa ttttgaggca
    11521 ttttgccgac atcgtaggat ttttaatatt acaccaactg ctaattatcg ccagaaatgt
    11581 cgctcaattt tacggtaatt gtctggttgc gcttgtctat aggtggagtt tacgtgttag
    11641 ctttttgccg ctcatcattg aagtcaaaaa aatatttcat cattttactg gcgctcgctg
    11701 caattgccgg actgggtact catgccgcct ggagtagcaa tggtttgcca cgtatcgaca
    11761 ataaaacatt ggccagactg gcacagcagc acccggttgt cgttttgttt cgccatgctg
    11821 aacgttgcga tcgttcaacc aatcaatgct tgtcagataa aacaggtatt acggttaaag
    11881 gtacccagga tgcccgtgaa ctgggcaacg cttttagcgc tgacatccct gatttcgatc
    11941 tttattccag taataccgtc cggaccattc agtcggcgac ctggttttcc gcgggtaaaa
    12001 agctgacggt agataaacga cttcttcagt gcggtaatga gatttatagt gcaattaagg
    12061 acttacaaag caaagcgcct gataaaaata tcgttatttt cacccataat cattgcctga
    12121 catatattgc taaaaataag cgtgatgcga catttaaacc tgattatctg gatggtttag
    12181 tcatgcatgt ggaaaaaggc aaagtttatc tggatgggga attcgttaac cactaaattt
    12241 tcaatctgac agccagtgat ggctgtcatc attgttaaag aagacctttc aaacgtaacg
    12301 tttttcgggt ggcaacattt aaatcataat gcaccagatc ttcaggtttt acccacgcgt
    12361 agtcctgaaa ctcttcgttt attttcactt ctcggttggc agaaacgcag tcaaaaatca
    12421 ggtaaatcat ataaatctct tccttgcgac catctgcata cgtcttggtg cgaatatcat
    12481 cgctgaaggt ccacggcgtg atttctgtca aaagcagctg ttctcccagt tcttcgcgaa
    12541 tttcgcggcg tagtgcctct tcaattcgtt cgccagactc cacgccgcca cccgaaagcg
    12601 cccattgacc ggggaaaacg ccgcgatcgt cggccatttt acacagcaaa taagcaccat
    12661 cattttgaat caaagggcat acaatagtcc gttgtcgcat aggggtattc cttataaata
    12721 aaaagaaacc atgagaatac ggtaactcag aaatatgtga aagtgacagt aatctctttt
    12781 gcaatgataa ctgcacttat tattattttt gtgtgtatcg gtaaagtgtg tttatttttt
    12841 cggcgtttaa ttatttaaga agtatttagt tacaataagt gactagatcc cttttttcgc
    12901 tcattgaatc ctctgtgcca ggcagtacaa tcccgcagct ttgggaattt tgattaattg
    12961 cttgtgggaa aggggataat gaaaaaaatt gcgctagcag gtctggccgg aatgctgttg
    13021 gtttctgcat cggtcaatgc aatgagcatc agcggccagg cgggtaaaga atacaccaat
    13081 attggtgtcg gttttgggac tgaatcgacg ggcctggctt taagcggtaa ctggacacat
    13141 aacgacgacg acggtgacgt cgcgggcgtg gggctggggt tgaatctgcc tctcgggccg
    13201 ttaatggcga ccgttggcgg aaaaggcgtt tacaccaacc cgaattacgg cgatgaaggt
    13261 tatgccgcag cggtaggagg tggtttgcag tggaaaattg gcaacagctt ccgtttgttt
    13321 ggcgagtatt actactctcc ggattcgctc tccagcggta ttcaaagtta tgaggaagcg
    13381 aatgctggcg cgcgttacac cattatgcgt ccagtcagta ttgaggcggg ttatcgctac
    13441 ctgaatctgt cgggtaaaga cggtaaccgc gacaacgccg tggctgacgg cccgtatgtt
    13501 ggggttaacg ccagtttctg atcccttccg gcgcggcatt ctgtcgcgcc attcagtttc
    13561 ttactacgcc ttatctccct ttgcgttata gtgattcgat aacaaaagca ggagataacc
    13621 atgttaaaag tggaaatgct atccaccggg gatgaagtgt tacacgggca aatcgttgac
    13681 actaacgctg cctggctggc cgattttttc tttcatcagg ggctgccatt atctcgccgc
    13741 aatacggtgg gggataacct tgatgactta gtcaccattc ttcgcgaacg tagtcagcac
    13801 gccgatgtgc tgatcgttaa cggcgggctg ggaccgacca gcgatgattt aagcgcactc
    13861 gccgctgcga cagcaaaagg tgaaggcatg gtgctgcatg aagcctggct caaagagatg
    13921 gaacgctatt tccacgaacg tggacgagta atggcaccga gcaaccgtaa acaagcggag
    13981 ctgcctgcca gtgctgaatt tatcaataac ccggtaggca ccgcctgtgg ttttgccgtg
    14041 cagcttaatc gttgcctgat gttctttact cccggcgtac cgtcagaatt taaggtgatg
    14101 gtcgagcacg aaatcctgcc gcgtctgcgc gagcgttttt ctttaccaca gccgccggtt
    14161 tgtctgcgtt taactacctt tggtcgttcg gaaagcgatc tggcacaaag cctggacact
    14221 ctacaactgc cgccgggcgt aacaatgggc tatcgctctt caatgccgat tatcgaactg
    14281 aaactcaccg gaccggcaag cgagcaacag gcgatggaaa aactgtggct ggacgttaaa
    14341 cgtgttgccg gacagagcgt gattttcgaa ggcaccgaag gactgcccgc gcagatcagt
    14401 cgcgaattgc aaaaccgcca gttcagcctg acgttgagcg agcaattcac cggtggttta
    14461 ttggctttgc aactttctcg cgcaggtgct ccattgctgg cgtgtgaagt ggttccttca
    14521 caggaagaaa ccctggcgca aactgcgcac tggattacag aacggcgggc caaccatttt
    14581 gccgggctgg cactggctgt ttcgggtttc gagaacgaac atctcaactt tgcgctagcc
    14641 acaccagacg gcactttcgc tctgcgtgtg cgtttcagca ctacgcgcta cagcctggct
    14701 atccgtcagg aagtgtgcgc aatgatggca ctgaatatgc tgcgccgttg gttaaacggc
    14761 caggatatcg ccagtgagca tggctggatt gaggttattg agtccatgac cttatctgtc
    14821 tgattccgcc actctgtgcc ggtttactgc accggcacat tccccctccg gcaaaacttg
    14881 tccaaccctc cctacggtga gcgtggtgtt ggggccagtg tggcacgggc tgcgcgctgg
    14941 ggacgcattg agaattacat ggcgcaagtc aacgattcgc tttgtctgtt agtgcaggtg
    15001 gaaagtaaaa cggcactgga taacctggac gagatcctcg acgtcgaagg gattgatggc
    15061 gtgtttattg gaccagcgga tctttctgcg tcgttgggct acccggataa cgccgggcac
    15121 ccggaagtgc agcgaattat tgaaaccagc attcggcgga tccgtgctgc gggtaaagcg
    15181 gctggttttc tggctgtggc tcctgatatg gcgcagcaat gcctggcgtg gggagcgaac
    15241 tttgtcgctg ttggcgttga cacgatgctc tacagcgatg ccctggatca acgactggcg
    15301 atgtttaaat caggcaaaaa tgggccacgc ataaaaggta gttattgata tcaaaggccc
    15361 atggggatcg gctgtgggcc tgtgttaatt agtggttatt cgctgccaga tcggcttcgc
    15421 ttagctgggt ggccgcgagc acctggtcac gatcaatgcc ctgttccagc atgcgcaaag
    15481 caattttaat ggcttgttca tgcatacctt cctgccagcc aatttgatgc ccttcctgcc
    15541 gtagccgttc agcaatagtc attaatctct ccttttgtag tggtgaacgt tcggcaatct
    15601 cctcaataaa acgggtgaaa cgggaggtat cgccgcattg cagcagataa ttaaacagtg
    15661 tttgtagctg gctgtcatta gtgaagcctc taaccaaaag cgtggtgatc ctgtcgacca
    15721 tgccgattaa atcgcggtcg cgaatatgct tttgaatcag ttccagcaga gctatacgcc
    15781 gatgttgcat gatctcatcg tcaggtacga tggtgatatc caccaacgga aaagcttcgg
    15841 tgtacaactg ccgggcaagt tgcggatcgt caaactcatc cagccagtta agtgagtacg
    15901 ggtagggcga tgtttcgcca tgataaaaca gcaatggcac caccagcgga actctgtcat
    15961 agcctttatc caggtgacgc tgcatggcgg cagtggcata gcgcattagc cgaaaagcca
    16021 tattcttttc tgcagagctt tgatgttcaa tcacgcagta gatatagccg tcaccgtcgc
    16081 tggtttccac ggaccacaaa acatccgagt agtaagcgcg taaacttttt tcaataaaac
    16141 tagtgggttc caggcgtaag gtttgcaggt tgcaaagctt gcgcagtggt tctggtaaat
    16201 gtatttcgag aaaatcccgt gcggtttcgg gtgtgaacat aaaggtttta aacaccgcat
    16261 catgcgggga ggatgttgtt gattcggtca tggttttccg tcacctgata tttatgatga
    16321 cgcgacatta cgtctatccc gggcctttga catctgtcag tttcagcctt tgcgagacat
    16381 tttcagagga attttcaaag cgttgcaacg catacatggt gcgtggaatc gcgctcgcaa
    16441 aaatgcccga taagcaatgt gcttaccggg catcaaaagg tcggttaagc cagcgcctgg
    16501 gccagtagcg taatcggatg ttcgcagcga aggctcgtgg acatctcaat ctgccatttg
    16561 caggtttcgc agtcggtgat gaccagatcc gcgccgctct cttctatctg gcggaacagt
    16621 ggcgcaccga tggcttgtga ggtggggtag ttctcttttt tgaaaccgta agtacccgca
    16681 ataccgcagc actgggaatc cagcaccgtt aactcaagcc ccgggatttt acgcaacagc
    16741 tccagggtgt agagcgtcca gcccattttt tccatatggc acggagtgtg ataaaccact
    16801 ttcagcggca gcggtttcag cggtaacgtt ttgccttcgt ccagcttgcg ccacagccag
    16861 cgggttgcca gttcgatatg atcgcgcaag cctttgttgt cgacattcag cacttccggg
    16921 tattcgtcgc gcagggcaaa tgtacaggtt gaggaggtgg caatcaccgg aatgcctttt
    16981 actcccacag cttcgcggat cgactctaca ttcgtaattg cctgtttgcg tgctttatcg
    17041 gtaaagccgt tggcgattag cggtacgccg cagcattttt ctttgctgag cagttgtaca
    17101 ccggtaccca ttgcgttgag cactttaatt aaatctttac ctaactgcgg atggttgtag
    17161 ttaacgaagc agccgtgaaa gaaagcgacc tggtctttat attgtgcttg ctgagccgct
    17221 atgctgcgat accagcgacg aaacgtaccg aaggagtatt tcggtagcgt gcggcgatga
    17281 tcgattttta acgccgcatc aagcagctgc cgcaccggtt tcagcgaggt agcggtgttg
    17341 acgattggtg cgaacggcgt ggaaacgcta cccatcaggt cggtatgact caacacaaaa
    17401 ttacgcagcg acgggcgcgt ggtgtcatat tttgcccgcg cgcgctggat aatatcgcca
    17461 atcttcacat cggacgggca ggcgacttca caacgtttgc agttgatgca atatttcagc
    17521 gcctcgtcat acagtgcgcc atctttcaaa cgcagacgct cgccatccgg cccggcttgt
    17581 tttggccctg gataaccggg attcacccgg ctcaccgggc aggcggtggt gcagacggtg
    17641 cacttaatgc agttttcgaa gctggtgtcg ttcattgttg gcctcctgcg cgttgggcaa
    17701 tctgttgagc ggcatgtaaa gcactgacgg cacaaacacc gccgccgcat ccctgggcga
    17761 tgggatcaaa tccgcccagc accgaaccga tggcaaacag gttctcaatg gtttgccctg
    17821 cctgcgacgg gcgtagcgtc tgatcagtgt ttacaccgaa ctgctgccac ggttgcggcg
    17881 caaaaaaatc gcccttatac cattcacccc gcgtggcggt ttgtagcaca tcaaggccga
    17941 gaatcggctc gcgaatgccg ttacgttccg ctaccagtcc gccactaaag aaactgccgc
    18001 tggcgagaac cgcgaaacgt ggacgtagcg gaatatcggc gtgattgcgg gtccagattt
    18061 cattcactac gccattttta caggtcactt ttttcacttc atcgcccggc atccacacgc
    18121 caccctggcg cacaaactgg cgctgtaact ggttttgcag acgaatgccc agcacggaag
    18181 gcggcagcgt tggcaaaagc atcagtgaac aaggtagttt ttcattcaac caacgccaca
    18241 gtttgtcatc ggccaggccg aagcaggcgg gcatcaggat catttcgcag gtattggcga
    18301 caggaataag cgcatcaagt aacagcggcc agttttcttc attatcaagg aaacgggcga
    18361 tattcaccgc gcgaaattcg gtggcgttat tgcgcaggac atccagttcc ggcagctcta
    18421 tttctgcggt ttcaaccgaa agatccagtt cgcgcaacga agccgccgca agatgcgcct
    18481 ggaaatccat cagcccgctt atccccacta cacatatctt cttcgcgggt agcggccaga
    18541 cgggaacttc tggcgaactt aaccaggttg cacgcaaagt gccgagcggg gtaacgcgct
    18601 gatgggccag ttccacgctg ccctgcaatt gcgcaccgct ttcagcgatc aatgcctgcg
    18661 cctggcaagc gagatcgagc acgcgttgtg gttcgagaag actgtaagga tgggctggtg
    18721 cctgctgacg caaagattcc agtccactgt gaatgtctgt caccggttga ccatcaggca
    18781 gatggctcag caaatccagc gaaccggatg agaaatgcag cgcgctttga ccacgcgtga
    18841 caatggcaca gcgcaggccg tgtttttgca gttgcaggcc acagagtaat ccggcgaggc
    18901 cgccgcccat aatgacagta tcaaagcgca tctttctgct ccttctccag accacacaat
    18961 ccctgataaa cccagcgggt aaattcgctt tcgcgcagtg catctcccca ggcgatgggt
    19021 tgcacgcctt tccagcgttc gttaaggaag gtggaaagtt gctcgataga ttgcgcggac
    19081 gtcgtgacgt taaaacgttg cagcagtccg gcagcgcggc aggcgcagag ttcgccctgg
    19141 caggtaccca tccccacacg ggtacgacgg cgtaaatcca gcaggctatt aacgtttaaa
    19201 ttttctaccg cgtactgaac ttcacccgca gttaccgctt cgcactcaca taccaggcta
    19261 cgatgcagac ggccttcgct cagccaggca ggcgtgcgat cgccatgacg ataaaccgca
    19321 gaaccgcgca gcggggcagg cagggagatg actttacgca aggtaacttc agcgggttct
    19381 tgtgaaccag gcagtgccag atcggcagtc gtacaggggc gcgtgttgcc cagtttgcgg
    19441 cataccgcgt cggtagccca ttcagccatc agccgatagg tcatcagttt gccaccggtg
    19501 atggtgataa atccgtccag accatcgcgt tcagcatggt cgagcagcac gatgccacgg
    19561 ctgacgttac gtccgctcgg gtcgtcatcg ctggcaacca gcgggcgcac gccagaatag
    19621 gcccgcaaaa tgcgcgtttt cgccatcacg ggggccagtt tttccccttc acgcagcaga
    19681 atatcaacct cttctgccgt cactcgatta tcgtcaatct cgttgtaatc aatacgtaaa
    19741 gaggtggtgc caatcagcga aatggtatcg ccaggcacca gaatatcggc gtcggaaggt
    19801 ttacggcagc ggttgatcac atgctggtta atgcggtgat ccatgatcag cagcgatcct
    19861 ttcgccggga acatgcgaat gcgcagatcg gcatattcgg caatgtgttg cccccagatc
    19921 ccagcggcat taaccacgac aggtgcatga agggcctgag tttcgccggt gagatggtta
    19981 cgtacacgaa caccgcacac cgtcgcgcct tcacgaatca gccccgtgac ttcatgagcg
    20041 gtaaggataa cggcaccgtg ttctttggca tccagcatgt ttgctgcggt cagacgaaat
    20101 ggatcaacgg tgccatccgg aactttcacc gcgccaatca gtgccgggtt aacggcaggt
    20161 tcgataatgc gcgcttgctg cgggtctata gcttctgcgc tgatccctgc ttcttcgcag
    20221 gcgcgaataa aagtggcctg gaatgagagg tcatcttccg gcagggtgat aaacaggccg
    20281 ttggttggtt caacgcagtg acgtgcaatg cgtttcagga tctggttttc actaatgcat
    20341 tcgcgggccg attccgcatc ggttaccgca tagcgcgcac cgctgtgcag caggccgtgg
    20401 ttacgcccgg tggcaccggt tgcgatgtcg tggcgctcaa ccaaaatcac gcgcagcccg
    20461 cgcagggcac agtcgcgggc aatcccggct cccgttgcgc cgccaccaat gataatcacg
    20521 tcacttgatt gcgagtcgcg agttttcatt gttatccctc tgaagttcgt tttttaccat
    20581 ttagccatag taaaaacatg aattgtttga tttcgcgcat attcgctcat aattcgaaag
    20641 tgaaacgtga tttcatgcgt cattctgaac attttgtaaa tcttatttaa taatgtgtgc
    20701 ggcaattcac atttaatttc tgaatgtttt cttaacatcg cggcaactaa agaaacggca
    20761 ggttctccca ctgaatcagt ctgttaatca taaataagac cacgggccac ggaggctttc
    20821 aatgttgagt atttttaaac cagcgccaca caaagcgcgc ttacctgctg cggagatcga
    20881 tccgacttat cgtcgattgc gctggcaaat tttcctgggg atattctttg gctatgcggc
    20941 ttactatttg gttcgtaaga actttgcgct tgctatgcct tatctggtcg agcagggatt
    21001 ctctcgcggt gatttaggtt tcgcgctttc ggggatctcg atcgcttatg gattttcgaa
    21061 attcataatg ggttcggtat cggatcgctc gaatccgcgc gttttcctgc ccgcaggttt
    21121 gattctggcg gcggcagtga tgttgtttat gggctttgtg ccatgggcga cgtcgagcat
    21181 tgcggtgatg tttgtactgt tgttcctctg cggttggttc caggggatgg ggtggccgcc
    21241 gtgtggtcgt actatggtgc actggtggtc gcagaaagaa cgtggcggca ttgtgtcagt
    21301 gtggaactgt gcgcacaacg tcggtggtgg tattccgccg ctgctgttcc tgctggggat
    21361 ggcctggttc aatgactggc atgcggcgct ctatatgcct gctttctgcg ccattctggt
    21421 ggcattattc gcctttgcga tgatgcgcga taccccgcaa tcctgtggct tgccgccgat
    21481 cgaagagtac aaaaatgatt atccggacga ctataacgaa aaagcggaac aggagctgac
    21541 cgcgaagcaa atcttcatgc agtacgtgct gccgaacaaa ctgctgtggt atatcgccat
    21601 cgccaacgtg ttcgtttatc tgctgcgtta cggcatcctc gactggtcac cgacttatct
    21661 gaaagaggtt aagcatttcg cgctggataa atcctcctgg gcctacttcc tttatgaata
    21721 tgcaggtatt ccgggcactc tgctgtgcgg ctggatgtcg gataaagtct tccgtggcaa
    21781 ccgtggggca accggcgttt tctttatgac actggtgacc atcgcgacta tcgtttactg
    21841 gatgaacccg gcaggtaacc caaccgtcga tatgatttgt atgattgtta tcggcttcct
    21901 gatctacggt cctgtgatgc tgatcggtct gcatgcgctg gaactggcac cgaaaaaagc
    21961 ggcaggtacg gcagcgggct ttaccgggct gtttggttac ctgggcggtt cggtggcggc
    22021 gagcgcgatt gttggctaca ccgtggactt cttcggctgg gatggcggct ttatggtaat
    22081 gattggcggc agcattctgg cggttatctt gttgattgtt gtgatgattg gcgaaaaacg
    22141 tcgccatgaa caattactgc aaaaacgcaa cggaggctaa tggcatgaaa ttgacgctga
    22201 aaaaccttag catggcgatc atgatgagca ctatagtcat gggaagcagt gcaatggcgg
    22261 cggacagcaa cgaaaaaata gtcatcgccc atcgcggtgc cagtggatat ttgccggagc
    22321 atacgctgcc agcaaaagcg atggcgtatg cgcagggagc ggattatctg gaacaggatt
    22381 tggtgatgac caaagacgac catctggttg ttctgcatga ccattatctc gatcgtgtta
    22441 ctgatgttgc cgatcgtttc ccggaccggg cgcgcaaaga cggtcgttac tacgcgatag
    22501 atttcacgct ggatgaaatt aagtcgctga aatttaccga aggtttcgat attgaaaacg
    22561 gtaaaaaagt gcagacttat ccggggcgtt tcccaatggg taagtccgac ttccgggtgc
    22621 acacctttga agaagagatt gaatttgttc aggggttaaa tcactctacc gggaaaaata
    22681 tcggtattta tcctgaaatc aaagcgccgt ggttccatca tcaggaaggg aaggatattg
    22741 cggcaaaaac gctggaagtg ctgaagaaat atggttacac cggtaaggac gacaaagttt
    22801 atttgcaatg ttttgatgct gatgagctga agcgtattaa gaatgagctg gaacctaaaa
    22861 tgggcatgga tctcaatctg gtacagttga ttgcctatac cgactggaat gaaacgcagc
    22921 agaaacagcc ggatggaagc tgggttaatt acaactacga ctggatgttt aagccgggtg
    22981 ctatgaaaca ggtggcggaa tatgccgacg gaatcgggcc ggattaccat atgttgattg
    23041 aggaaacatc gcagccgggt aatatcaaac tcactggcat ggtgcaagat gctcagcaga
    23101 ataaactggt agtgcatcct tataccgtgc ggtcagataa actgcctgaa tacactactg
    23161 atgtgaatca gttatatgat gctctgtata acaaagcggg tgtaaatggg ctgtttactg
    23221 atttccctga taaggcagta aaattcctta ataaagagta atgctactgc aggatggcgt
    23281 tcatgcgcca tcctgtctca catcaaattt ttccattcac cacgagttgt tccgtacacg
    23341 tttgtagtaa aaacagtgga ctgccttcta ccggcttttc tttagccaac aacgaaatct
    23401 gtcgatagaa ggttggttcg tgaggaacgg aacacaattc acctgcaatg cttttcagcg
    23461 ttaactctgg ttgtaaggcg atgcctaatc cctgtcgaat aaacttcatc gctgtatccg
    23521 actgattaaa ttcacatttt atctactggg cgcactatta gtggtagtgg ctggtggaat
    23581 ctatgcgctg caaatgaagg gtaaaagcgg tgtcacgcta gtagtggcaa aagaaattca
    23641 ttaatacgac acgtttcatt aagattttcc tcaggtaacc acctatagtc attgtcgtta
    23701 ataaaaatga caggcgatag ggtatggcag tttcagcaaa gtacgacgaa tttaatcact
    23761 ggtgggcaac agagggcgac tgggttgaag agcctaacta tcgccgtaat ggtatgagcg
    23821 gggtgcaatg cgtcgagcgc aacggcaaaa agctgtatgt aaagcgcatg acgcatcatc
    23881 tgtttcattc cgtacgttat ccgttcggcc gaccaacgat tgtccgtgag gttgcggtaa
    23941 ttaaagaact ggaacgggcc ggtgtcatcg tgccgaagat cgtttttggt gaagcggtga
    24001 aaattgaggg tgaatggcga gcgttgctgg tgactgaaga tatggcgggg ttcatcagca
    24061 ttgctgactg gtatgcccag catgcagtat cgccttattc tgacgaagta cggcaagcca
    24121 tgttgaaagc agtggcgctg gcgtttaaga aaatgcatag cattaatcgt cagcatggct
    24181 gttgttatgt tcgccatatc tatgtgaaaa cagaaggtaa tgcagaagct ggttttcttg
    24241 acctggagaa aagtcgtcgc cgcttgcgtc gcgataaagc gattaatcat gacttccgcc
    24301 agctggaaaa atatctggag ccaatcccca aagcggactg ggagcaggtg aaagcgtatt
    24361 actacgccat gtaatcgttg taggccagat aagacgcata agcgttgtat ctggcactac
    24421 aaccaattca catctcgatt tcaatatcgc cttttgcccg gcaacaacag ggcaaaattt
    24481 cccccggctg aataaaggct aacggttcgg caatccagtc aacctgacct gcaaccagac
    24541 gggtgcgaca ggagccgcag taaccttcgc gacactgata ctcaaccgcc acattgtggg
    24601 attccagcgc cgccagaagg gaagggtgtt catcctggca cagcagttgt gtgccagtga
    24661 tgcgcagggt aacgcgggcc atcagagttg gaagttactc aaatcgtcgg tgtccacttc
    24721 cgcgtcaatc tgcccgacca gataagaact gacttccact tcctgcggag caacctgcac
    24781 gttatcagac accagccagg tgttgatcca tgggatcggg ttggagcgcg tctggaacgg
    24841 cagatccaaa ccgactgcct gcatacggat attggtgatg tattcaacgt actggcagag
    24901 aatgtcttta ttcagaccaa tcatcgaacc gtcgcggaac agataatccg cccagtcttt
    24961 ctcctgttga gctgcctgaa caaacaggtc atagcactcc tgcttacact cttcggcaat
    25021 ttccgccatc tcaggatcgt ccgcgccgct gcgcagcaga ttcagcatat gctgggtgcc
    25081 ggtcaggtgc agggcttcgt cgcgggcaat caggcgaata attttggcgt tgccttccat
    25141 caattcgcgt tctgcaaatg cgaaggaaca agcaaagctg acgtagaaac gaatcgcttc
    25201 cagcgcgtta acgctcatca ggcagagata cagttttttc ttcagctcgc gcaggctaac
    25261 ggtcacagtt ttaccgttaa cggtgtgggt accttcgccc agcagatgcc agtagctggt
    25321 catttcgatc agctcatcgt aatagctgga gatcccttcc gcacgtttct ggatctgctc
    25381 gttggtgacg atatcgtcaa acacaacaga cggatcgtta acgatattac gaatgatatg
    25441 agtataggaa cgggaatgaa tcgtttctga gaacgcccag gtttcgaccc aggtctccag
    25501 ttccgggatc gagatcagcg gcagcagtgc cacgttcggg ctacggccct gaatggaatc
    25561 cagcagcgtc tgatatttca ggttgctgat aaagatgtgt ttttcgtgtt caggcagtgc
    25621 ctggtaatcg atacggtcgc gagaaacgtc aacttcttcc ggacgccaga agaaagagag
    25681 ctgcttttcg atcagctttt cgaagatgtc atatttttgc tgatcgtagc gagccacgtt
    25741 gaccggctga ccaaagaaca tcggttcttt gagctgatca tttttcgtct gtgaaaaggt
    25801 ggtatatgcc atgagtgtgt cctgtggttt tattacccct caccctaacc ctctcccgca
    25861 aggggagagg ggacggtttg gtgcaaaggc tgggggatta tcagatctta catgcgccgc
    25921 tttcgcagcc atcgtcctgg attgacggta ccagatcgtc ctgcgcgtct tcagcgccgt
    25981 cacgggtgtt ctgataatac agcgttttga ccccgaattt gtaggcggtg agcaggtctt
    26041 tcagcaactg ctgcatcggc acttttcctg acgggaaacg tgacggatcg tagttggtgt
    26101 tagcagagat cgactgatcg ataaatttct gcatgatacc taccagttgc aggtagccat
    26161 cgttacccgg catttcccac agcagctcat aggcgtcgtg caggtgctcg tagtccggca
    26221 ccacctggcg caaaataccg tctttcgacg ctttgatgct gacgtaaccg cgcggcggtt
    26281 caataccgtt agtggcgtta gagatctgtg aagaagtctc tgacggcatc agggcagaaa
    26341 gcgtggagtt acgcaggccg tgcgttttga ttgattcacg caatgcttcc cagtcgtaat
    26401 gcagcggctc attagcgatg gtatccagat ctttcttata ggtatcgatc ggcaggatcc
    26461 ctttcgcgta agtggtttcg ttaaaccacg ggcacgcgcc ttgctctttt gccagctcgt
    26521 tagacgcttt cagcaggtaa tactgaatcg cttcgaaggt tttatgcgtc aggttgttgg
    26581 cgctgccgtc ggagtaacgc acaccgtgct tcgccaggta gtaagcgaag ttgatcacac
    26641 caatacccag cgtacgacga cccatcgctc cacgtttggc ggccgggatc gggtaatcct
    26701 gataatccag cagcgcgtca agtgcacgaa ccgccagaat tgccagctct tccagttcat
    26761 ccaggctatt aattgcgccc aggttgaaag cagacagcgt acacagcgcg atttcaccgt
    26821 tctcgtcgtt gacgtcgttc agcggtttgg tcggcagggc tatctccagg cacaggttag
    26881 actgacgcac tggcgcgatg gccggatcaa acgggctatg ggtattgcag tggtcaacgt
    26941 tctgaatata gatacgaccg gtagacgcac gttcctgcat catcagcgag aacagctcaa
    27001 cggctttcac acgctgcttg cggatgctgt cgtctttctc atatttggta tacaggcgtt
    27061 caaactcttc ctgatcggcg aagaatgcgt cgtacagccc cggtacgtcg gacgggctga
    27121 acagggtgat atcttcacct ttcagcaggc gggtatacat cagtttgttg atctgtaccc
    27181 cgtagtccat atggcgcacg cggttgcctt ccacaccacg gttgtttttc aacaccagca
    27241 ggctttccac ttccagatgc cacatcgggt agaacagcgt tgccgcaccg ccgcgcacgc
    27301 cgccctgaga gcaggatttc accgctgtct ggaaatgttt gtagaacgga atacagccgg
    27361 tatggaacgc ttcaccaccg cgaatcgggc tacccagcgc acgaatacgc ccggcgttga
    27421 tgccgatccc ggcacgctgg gaaacgtatt tcacaatcgc gctggaggta gcgttgatgg
    27481 aatccagact gtcaccgcac tcgatcagta cgcaggagct gaactgacga gtcggggtac
    27541 gcacgccgga catgattggc gtcggcagcg aaattttaaa tgtggaaacc gcgtcgtaaa
    27601 aacgcttcac atattgcagg cgcgtttcac gcgggtagtt cgagaacagg cacgcggcaa
    27661 ccagaatata aaggaactgg gcgctttcgt agatttcgcc ggtcacgcgg ttctgtacca
    27721 gatatttgcc ttccagctgc ttaacggcag cataagagaa ggtcatatca cggtcgtgat
    27781 cgataaacgt gtccatctgc ttgaactctt cttccgtgta gtcttccagc agatgattat
    27841 cgtatttgcc catctcgacc attttcacca cgtggtcgta cagcgcaggc ggctcaaact
    27901 ggccgtaggc ttttttacgc aggtggaaga tcgccaggcg cgcggcgaga tactgataat
    27961 ccggcgcatc acgggagatc aggtctgcgg ccgccttgat aatggtttcg tggatgtcag
    28021 aggtcttgat accgtcataa aactgaatgt gggagcgcag ctcgacctgg gaaatcgaaa
    28081 cgttatgcag tccttctgcc gcccaatcca gaacgcgatg gattttgtcg agattgatgc
    28141 gctctgtgct accgtcgcgc tttgtcacca gcagattctg attcatgtat gtcgtacctg
    28201 tttttggaat tctttccgca atagtttcat gcagactacc tgtagtacgg gaagctgctt
    28261 agaacactat atataggggg tatgtttggg attcactgca agatagtgtg aaaatggccc
    28321 tcttgcaagt gcataacttt gtggataact caggaaggaa aaagtggctt tcgcgcacct
    28381 taggtcagac aaggtgtccg ggaaagtcaa tgggaagaga aaaatttgtt aaaaataact
    28441 gttcggcgat ttcttgaacg gcagaatagg ttggataaat tgatatagat ggtttattaa
    28501 tctttttgct aatgattgca aaaattgatc taagcacgag tcatcacata atctgatgta
    28561 tcagttgtgg tcaatatata agcgaatgta agcgattgtt gatgtcgaaa tggttgtttt
    28621 ctattcggtt attgttaaat gcgaagccat tttgttaatt taccttatat tataagaaaa
    28681 ttattcatta ctttaattta gtttaaatat aactggtatc ccttaaggtt attaacttga
    28741 attatgacgc actgatacta ttcatcaaat aataacaaaa tagccattgc atcgggttgg
    28801 acaatttaca aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata
    28861 taaaatgatg gttagatgaa tatgcggatt atctttctac gcaaggagta tttatctcta
    28921 ctcccgtcaa tgattgcatc tcttttctct gctaacggtt tcgcggcggc cattgattta
    28981 tgccagggat atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt
    29041 acgcaggact ggagtgtcgc cgatgggcaa tggctggttt tttcggatat gaccaataac
    29101 gccagcggag gagccgtatt tttgcaacaa ggagcggaat tttcactatt accagaaaat
    29161 gaaactggaa tgactctgtt tgccaataac accgttacag gagaatataa taacggcggg
    29221 gccatatttg ctaaagaaaa ctcaacgctg aatcttactg atgttatttt ttctggtaac
    29281 gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaatgatac tggtgtcgtc
    29341 gatttacgtg tcactaacgc catgtttcgc aataacatcg ctaatgatgg caaaggtggc
    29401 gcaatttata ccattaataa tgacgtttat ttaagtgatg ttatttttga taacaaccag
    29461 gcatatacat caacaagtta cagtgatggc gatggcgggg caatcgatgt taccgataat
    29521 aatagcgaca gcaagcatcc ttcaggttat acgataataa ataacaccgc ctttacaaat
    29581 aacactgccg aagattatgg cggggcgata tataccaata gcgcgacggc tccctatctt
    29641 attgatattt ctgttgatga cagctacagc cagaacggcg gcgtgttagt ggatgagaac
    29701 aacagcgcag caggctatgg agatggtcct tccactgcgg cgggtggctt tatgtatctc
    29761 ggcttaagtg aagttacctt tgatattgcc gacggaaaaa cgctggttat tggcaataca
    29821 gagaatgacg gagctgttga ctctattgct ggtaccgggt tgatcaccaa aacaggttcc
    29881 ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac
    29941 ggtgaagtta ccctgggtcg cagcaactcc ctgatgaatg tcggcgatac gcattgccag
    30001 gacgatccgc aagactgcta cggtctgacg atagggagta ttgataagta ccagaatcag
    30061 gcagaactaa acgttggctc cacccaacaa acctttgtgc actcattgac gggctttcag
    30121 aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg cagttttgct
    30181 ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta cgtgctgtcg
    30241 ggggcgcagt cgatggcgct aaccggcgat atagtcgttg atgatggtgc ggtgctttcg
    30301 ctggaaggcg acgcggcaga tcttaccgct ctccaggacg atccgcagtc gatcgtgtta
    30361 aacggcggtg tgctcgatct ctctgatttc tccacctggc agagtggcac atcatacaac
    30421 gatggccttg aagtcagcgg cagcagcgga acggttatcg gcagtcagga tgtggtagat
    30481 cttgcaggtg gaaacgatat gcatatcggc ggtgacggga aagatggcgt ctacgtggtg
    30541 gtcgatgcgg gcgacgggca ggtaagtctg gcaaacaata atagttattt gggcacaaca
    30601 caaatcgcct ccggtatgct ggaggtgagc gacaactcac agcttggaga tacccactat
    30661 aaccgccagg ttatctttac cgataagcaa caagaaagcg tgatggagat tactgccaat
    30721 gtcgatactc gctctacgac gactgagcat gggcgtgata ttgaaatgcg cgccgacggt
    30781 gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc
    30841 gggcagcatc aggatgaggg tagcacattg actaaaacgg gggcgggtac gctggagctg
    30901 accgccagcg gtacaacgca gtcggcggta cgtgtcgaag aaggcaccct gaaaggcgat
    30961 gtcgcggata tccttcctta tgcttcgtca ctgtgggtcg gtgatggggc gacgttcgtt
    31021 actggcgcgg atcaggatat tcaatctatt gatgctactt ccagcggcac tatcgacatc
    31081 agcgatggta cggttttgcg cctgaccggg caggatactt ctgtcgccct taatgcctca
    31141 ctgtttaacg gcgatgggac gctggtgaat gccaccgatg gcgtgacgtt gacaggtgag
    31201 cttaatacca accttgaaac tgacagcctg acctatcttt ccgacgtgac ggttaatggc
    31261 aatctgacca atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg cgatacgctg
    31321 acggtaaacg gtgattatac cggcggcggt acgctactgc tcgatagcga attaaacggc
    31381 gatgactcgg taagcgatca attggtgttg aacggtaata ctgctggcaa cacaactgtg
    31441 gtggttaact ccattacagg gattggtgag ccgacatcga caggcattaa agtggttgat
    31501 ttcgcagccg atcccacgca gtttcaaaac aatgcgaagt tcagtctggc aggcagcggc
    31561 tacgtcaata tgggagcata tgactacacg ctggtggaag ataacaacga ctggtatctg
    31621 cgatcgcaag aagtaacgcc accattgcca cctgatccag accctgaccc aacacccgat
    31681 cctgacccaa cacccgatcc aatccccgct taccagccgg tgctgaatgc caaagttggc
    31741 ggttatctca ataatctgcg ggcggcaaat caggcgttta tgatggagcg acgcgatcat
    31801 gcaggtggca atggtcagac gctgaattta cgtgttatcg gcggacgtta tcattacacg
    31861 gcggcagggc aactggctca gcatgaagac acttctacgg tgcagcttag cggtgacctg
    31921 tttagcgggc gctggggcga tgatggcgag tggatgcttg gggcggttgg tggctacagt
    31981 gataatcagg gcgagagccg ctcgaatatg accggaactc gcgccgataa ccagaaccac
    32041 ggttatgccg ttgggctgac ctcaagctgg tatcagcacg gtaatcagaa gcagggggcc
    32101 tggctggata gctggctgca atacgcgtgg tttaacaatg atgtttccga acaagacgat
    32161 ggcacagatc attaccactc gtcggggatt atcgcctcgc tggaagccgg gtatcagtgg
    32221 ttaccggggc gtggtgtggt gattgaaccg caggcgcagg tgatttatca gggcgtgcag
    32281 caggatgatt ttaccgccgc taaccatgcg cgcgtgtcac aatcgcaggg ggatgatatt
    32341 cagacgcggc tgggtttaca cagcgaatgg cgtaccgcgg ttggtgtcac accaacatta
    32401 gatctgaatt attatcacga tccccatgcg acggaaattg aagaagatgg cagcactatc
    32461 agtgacgatg cggtgaagcg acggggtgaa ataaaagtgg gagtcacggg caatatcagt
    32521 cagcgagtgt ccctgcgcgg cagcgtggcg tggcagaaag ggagtgatga ttttgcccag
    32581 acggcagggt ttttgtcgat gacggtgaaa tggtaatcac tgtctagtcg gcacggttgc
    32641 cggatgcggc gtaaacgcct tatccggcct acggggagca tttgtaggcc tgataagacg
    32701 cggcaagcgt cgcatcaggc attggctcgg ccaaacaaca acctcactta ttctgcgtgt
    32761 gcagcatata gttcacatcc acgccggggc cgagtttaaa agtattagtg agcgggttgt
    32821 aatgtagccc ggttatatgt cgctctttaa ggcttgtctg atctacccag ccaagcagtt
    32881 ctgccggttt aataaacttc ttcacatcat gcgtgccttt gggcaccatg cgcaaaatat
    32941 attccgcgcc aaccaccgcc atcagccatg acttgccgtt gcggttaagc gtcgagaaaa
    33001 agacatcgcc gcctggtttc accagttgcg cacaggctct gaccactgac tgcggatcgg
    33061 ggacgtgctc cagcatctcc atgcaggtca ccacatcata ctgcccggca tgttttgccg
    33121 cgtgctcttc cacggtttcc tgcacataat ccacctgaat gccgctttcc agcgcgtgca
    33181 gttttgccac ctgcaatggc tcaaagccca tatccagacc ggtcaccgtc gcgccttcgc
    33241 gcgccatact ctcggccaga atgccgccgc cacaaccgac atcgagcacc tttttgccaa
    33301 ataaaccgcc agcacgctcg gcaatatagc ccagacgcag cgggttaatg cggtgcaacg
    33361 gtttgaactc accttcaaga tcccaccagc gggaggcgac ggcttcaaat ttagcgatct
    33421 cttcgtggtc tacgttatgg ttttccggcg atttttcggc attcattggc acttctactc
    33481 cgtaattggc aagacaaacg agtatatcag gcattggatg tgaataaagc gtataggttt
    33541 acctcaaact gcgcggctgt gttataattt gcgacctttg aatccgggat acagtagagg
    33601 gatagcggtt agatgagcga ccttgcgaga gaaattacac cggtcaacat tgaggaagag
    33661 ctgaagagct cctatctgga ttatgcgatg tcggtcattg ttggccgtgc gctgccagat
    33721 gtccgagatg gcctgaagcc ggtacaccgt cgcgtacttt acgccatgaa cgtactaggc
    33781 aatgactgga acaaagccta taaaaaatct gcccgtgtcg ttggtgacgt aatcggtaaa
    33841 taccatcccc atggtgactc ggcggtttat gacacgatcg tccgtatggc gcagccattc
    33901 tcgctgcgtt acatgctggt agacggtcag ggtaacttcg gttccatcga cggcgactct
    33961 gcggcggcaa tgcgttatac ggaaatccgt ctggcgaaaa ttgcccatga actgatggcc
    34021 gatctcgaaa aagagacggt cgatttcgtt gataactatg acggcacgga aaaaattccc
    34081 gacgtcatgc caaccaaaat tcctaacctg ctggtgaacg gttcttccgg tatcgccgta
    34141 ggtatggcaa ccaacatccc gccgcacaac ctgacggaag tcatcaacgg ttgtctggcg
    34201 tatatcgatg atgaagacat cagcattgaa gggctgatgg aacacatccc ggggccggac
    34261 ttcccgacgg cggcaatcat taacggtcgt cgcggtattg aagaagctta ccgtaccggt
    34321 cgcggcaagg tgtatatccg cgctcgcgca gaagtggaag ttgacgccaa aaccggtcgt
    34381 gaaaccatta tcgtccacga aattccgtat caggtaaaca aagcgcgcct gatcgagaag
    34441 attgcggaac tggtaaaaga aaaacgcgtg gaaggcatca gcgcgctgcg tgacgagtct
    34501 gacaaagacg gtatgcgcat cgtgattgaa gtgaaacgcg atgcggtcgg tgaagttgtg
    34561 ctcaacaacc tctactccca gacccagttg caggtttctt tcggtatcaa catggtggca
    34621 ttgcaccatg gtcagccgaa gatcatgaac ctgaaagaca tcatcgcggc gtttgttcgt
    34681 caccgccgtg aagtggtgac ccgtcgtact attttcgaac tgcgtaaagc tcgcgatcgt
    34741 gctcatatcc ttgaagcatt agccgtggcg ctggcgaaca tcgacccgat catcgaactg
    34801 atccgtcatg cgccgacgcc tgcagaagcg aaaactgcgc tggttgctaa tccgtggcag
    34861 ctgggcaacg ttgccgcgat gctcgaacgt gctggcgacg atgctgcgcg tccggaatgg
    34921 ctggagccag agttcggcgt gcgtgatggt ctgtactacc tgaccgaaca gcaagctcag
    34981 gcgattctgg atctgcgttt gcagaaactg accggccttg agcacgaaaa actgctcgac
    35041 gaatacaaag agctgctgga tcagatcgcg gaactgttgc gtattcttgg tagcgccgat
    35101 cgtttgatgg aagtgatccg tgaagagctg gagctggttc gtgaacagtt cggtgacaaa
    35161 cgtcgtactg aaatcaccgc caacagcgca gacatcaacc tggaagatct gatcactcag
    35221 gaagatgtgg tagtgacgct ctctcaccag ggctacgtta agtatcagcc gctttctgaa
    35281 tacgaagcgc agcgtcgtgg cgggaaaggt aaatctgccg cacgtattaa agaagaagac
    35341 tttatcgacc gactgctggt ggcgaacact cacgaccata ttctgtgctt ctccagccgt
    35401 ggtcgcgtct attcgatgaa agtttatcag ttgccggaag ccactcgtgg cgcgcgcggt
    35461 cgtccgatcg tcaacctgct gccgctggag caggacgaac gtatcactgc gatcctgcca
    35521 gtgaccgagt ttgaagaagg cgtgaaagtc ttcatggcga ccgctaacgg taccgtgaag
    35581 aaaactgtcc tcaccgagtt caaccgtctg cgtaccgccg gtaaagtggc gatcaaactg
    35641 gttgacggcg atgagctgat cggcgttgac ctgaccagcg gcgaagacga agtaatgctg
    35701 ttctccgctg aaggtaaagt ggtgcgcttt aaagagtctt ctgtccgtgc gatgggctgc
    35761 aacaccaccg gtgttcgcgg tattcgctta ggtgaaggcg ataaagtcgt ctctctgatc
    35821 gtgcctcgtg gcgatggcgc aatcctcacc gcaacgcaaa acggttacgg taaacgtacc
    35881 gcagtggcgg aatacccaac caagtcgcgt gcgacgaaag gggttatctc cattaaggtt
    35941 actgaacgta atggtttagt ggttggcgcg gtacaggtag atgactgcga ccagatcatg
    36001 atgatcaccg atgccggtac gctggtacgt actcgcgttt cggaaatcag catcgtaggc
    36061 cgtaacaccc agggcgtgat cctcatccgt actgcggaag atgaaaacgt agtgggtctg
    36121 caacgtgttg ctgaaccggt tgacgaggaa gatctggata ccatcgatgg cagtgccgcg
    36181 gaaggggacg atgaaatcgc tccggaagtg gacgttgacg acgagccaga agaagaataa
    36241 ttttacttct tcatgccaaa agggagctat ctcccttgtt tgaattgaaa agtccaggct
    36301 gcaaagtctg ggcttttgtc gtattagggc acggtaaagt ttggctgtgc ccgtaaaaaa
    36361 tggctggcta tacacaagga atgtggcaat gagtggtgaa aaaaaggcga aaggctggcg
    36421 gttctatggt cttgtaggtt ttggcgcaat agcactgctt tccgctggcg tctgggcgtt
    36481 gcaatatgct ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct
    36541 gcaaatcgat ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc
    36601 caaagatctc ctcaccattc cgtttctcca cgatgttctg agcgaagatt tcgttttcta
    36661 ttatcagaat catgccgatc gtctgggcat tgaaggcagc attcgtcgta ttgtctatga
    36721 acacgatctc acgctgaaag ataagctctt ttcgtcactc ttagatcagc ccgcgcaggc
    36781 agcgctgtgg cacgataaac aaggccatct ttcacattat atggtgctga tccagcgcag
    36841 tggtttaagc aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag
    36901 caaaacggaa atcagtagca tcaagataaa tagtgaaact gttcctgttt atcagttgcg
    36961 ctataacggc aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc
    37021 cagcacggat atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg
    37081 tgatttgttg agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtactgc
    37141 tgaaaaaacg ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta
    37201 ccagcggtta atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca
    37261 cagttttgtg gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc
    37321 ggtctggaac agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg
    37381 tattgccgaa gagatgcttt cgcacatcag tcaggaaaac gacaagttga atggggcgtt
    37441 agacggtgcc gcggggctgt gctggtatgc agactcaaaa ttgcaaaccc cgctgtttgt
    37501 cggtcagttt gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa
    37561 tattggtgcg cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca
    37621 gggcgaagcg caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc
    37681 gcaggcggcg caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat
    37741 gcaaaacaaa acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac
    37801 actgaataaa aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct
    37861 ctatatcaat ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc
    37921 gaagaatctc gaaccggttt tttataacgc cgcacaaact ttattaatgc cgaagctgga
    37981 cgctttatct caacaaccgc gttatgtcat gaagctggcc cagatggaac ccggtgccgc
    38041 ctggcagtgg ctacccataa cctggcagcc gctatgaggc acgggctgct ggcgctgatt
    38101 tgctggctgt gttgtgttgt tgcccatagc gaaatgctga atgtcgaaca atccgggctg
    38161 tttcgcgcct ggtttgtacg cattgcacaa gaacagctcc gccaggggcc aagtccacgc
    38221 tggtatcagc aggattgtgc gggcctggtg cgatttgcgg cgaacgagac gctgaaagtt
    38281 cacgatagta aatggctaaa aagtaacggt ttctctagcc agtatttgcc gccagagatg
    38341 acgctaacac ctggacagcg tcaactggcg caaaactgga atcaggggaa cgggaaaacc
    38401 ggcccctacg tgaccgcgat taatttgatt cagtacaaca gccagtttat tggccaggac
    38461 ataaaccagg cgctgcctgg cgatatgatt tttttcgatc agggcgatgc ccagcactta
    38521 atggtctgga tggggcgtta cgtcatctac cacaccggaa gcgccacgaa aactgacaac
    38581 ggaatgcgcg cagtcagtct gcaacaactt atgacatgga agttgtatat taaaatttca
    38641 ttaattatca atatgttaat gtgcgctggt cattattaat caaaataaaa ccattatgat
    38701 atttcaattt tgtcccactc ccgcccgcga ctgtccctgt aacgcgccgc cattgaatct
    38761 gatttatgcc cgagaagacg ttgagcaaac ttatcgccaa tctgattccg gtatagcctc
    38821 gccgacaggc tgcgcagttc atggaatgtt ggcgggtctc catcaaatga gagtccagat
    38881 gcatttctcg cctttgtaaa atacttagat actgttttcg gggaaagcgg ttcgtgatgc
    38941 gttgatgcaa ttattgtttc actgccgctg gcctccctgc atttctgtag tgtatcagcc
    39001 aatgagatat tgagcgcgtc aatcgttagc gtcagcggaa tggcgagctt agcccctgtt
    39061 ttaccctgct caatgtgaag atggttgtcg tttatgtctg accatttcat tctgcataaa
    39121 tcgcctactc tctgccctgt aacgacagcc aaatccattg ccagcctcag ccagattggg
    39181 agcggttcgg ctgcatggta aatcgcgaca tattcattag ctgtcagtct tgaacgcctt
    39241 acttctgatt ttgctgtgcg ggttgctgtt accggattcg tagccacatg cccctcggct
    39301 attgcctcac gaaaaacgtc aacaagggtt gacctgatta attttgcgga agccgcttta
    39361 ccttctgcta cgtaggtgtt tagcattgct gccacctctt tcgttgatat gtcagcgagc
    39421 ggtttgtccg gcaattttct tcggattgcc ctgattttgc tggcgtagtc gagtagagtt
    39481 ttcggcctga tacccctctc gctgaggatt gtttcatatc ggtcaagcca cgcatgaaga
    39541 gtgattgcgt cagcgccttt aattctgtct atcagtgact cacgcctgtt cccggatagc
    39601 aactcaatat tggcctgtat tgcttcagtg attgctatcc tcctgtctcg gcctaatccg
    39661 aactctttac ccgtccttgg gtccctgtag cagtaatatc cattgtttct tatataaagg
    39721 ttagggggta aatcccggcg ctcatgactt cgccttcttc ccatttctga tcctcttcaa
    39781 aaggctactt gttactggtc gatttaagtc aacctttacc gctgattcgt ggaacagata
    39841 ctctcttcca tccttaaccg gaggagggaa tatcctgcat tcgcgcaccc atcgacgaac
    39901 tgtttcaagg cttcttgggc gtcgctggcg tgcattccac tcctgaagtg tcaagtacat
    39961 cgcaaagtct ccgcaattac acgcaagaaa agccgcattg atgcggcaat ggtaggtctg
    40021 gatatcttga gaaatgaaca ggcctcattg agtgtgaggc tgtggttagt ccttgcgtag
    40081 ctcgctaatt cttctgtaag tctctggtgc tttgtttccg tgtatcttca tttcagactt
    40141 caacagagcg acgagggaat cccattcgtt gaggatgcct ttgaatgccg gaacgcgctt
    40201 tgcaaccttg tcgaatgaat ctctgatttc tggaatctgc tcaacaagtg caacgcatcg
    40261 ccggaagtct gctgcgtcat gtggagcgcc gaagtgatga ccatagatat tctttttcag
    40321 tccacatgcg attgaggcaa gagttgcgct actgatgcca acatcgccag tcgattgcca
    40381 tttcaaaacc ttcatagcca aatctgacat ttcttgtctc cataaaacaa aacccgccgt
    40441 agcgagttca gataaaagaa atccccgcga gtgcgaggat tgttattcat tgccgatatt
    40501 cacctttatc gcgaacacct ttaccggttt atctcctttg catggcgcgt aattttttca
    40561 gatggttctc ctgctctgtt tcagccagaa tttgtcggta ttcctggtga tcgatccgtt
    40621 caaacagttc attaaaatcg tttattttta ccgactgtgt tcgcccatcc attcttctgt
    40681 acaacacagt gttgtttatg cagcgaagaa tttttatcgg gtagccggcg ctatcggtgt
    40741 atatctgacc acgttgaatc agagcgaaca ttcctttatc cccagcggaa aagcgaatac
    40801 agaataaatg ccaccgcgat tgcaactcct acagcggtga atgcttcagg ccaattcatc
    40861 attcactccc tgcggcggtt ctggtagcag catccagtac aaggcgttcc ctaaccacga
    40921 taaagtgccg tcgctcaact ccacgtattc ctcttgtacc tgtcctgcca tatactcacc
    40981 gtgctttgaa taaattaaaa tccaatcatc ttgagcgggc attcgctcac tacagcttat
    41041 ccaaccaccc ggacttaccg gagagttgcc agtcttacgc atggcaatct ccacgatttc
    41101 aaccatatct cctggcagaa ttttacaaag ctggccaaag cgcctctgct gcctggcata
    41161 ctcgaggatg tgctccagtt tggttcgatt aatcatgatt tatctccctt aagcatggct
    41221 gcacggcagg cgttccagcc tctcacctct gcaatagctg caacagcatc aactgtgtac
    41281 atgctaagag ggttaggcat tggtttttct tccggtacta ctggcgatgg ctgtttggct
    41341 tctaaatcag caattctgtc aactacggca tcgacagcat ctgaaaaact gaaacagtta
    41401 ctccactcag gcctatcccc ggttgttgcg aagtacatat cagctaaagc agactcagca
    41461 tggtcacgct cgttaatgag ttgctcttcg cttttctcca gttcagcaat acgtttactc
    41521 ccatccgaga taacaccttc gtaatactca cgctgctcgt tgagttttga ttttgcttcc
    41581 tcaagctcaa cacgcagctt ccctaccgtt agcgcaattt cctcgttctc ctggtcgcgg
    41641 cgtttgatgt attgctggtt tctttcccgt tcatccagca gtgccagcac aatcgatggt
    41701 gttaccagct catggaaaag gtccgcatca aatccccagt cgtcatgcat tgcctgctct
    41761 gccgcttcac gcagtgcctg agagttaatt tcgctcactt cgaacctctc tgtttactga
    41821 taagctccag atcctcttgg caacttgcac aagtccgaca accctgaacg gccagacgtc
    41881 ttagttcatc tatcggatcg ccacactcac aacaatgagt ggcagatata gcctggtggt
    41941 tcaggcggtg catttttatt gctgtgttgc gctgtaattc ttcaatttct gatgctgaat
    42001 caatgatgtc tgccatcttc cattaatccc tgaattgttg gttaatacgc ttgagggtga
    42061 atgcgaacaa taaaaaagga gcctgtagct ccctgatgat tttgcttttc atgttcatcg
    42121 ctccttaaag acgccgttta acatgccgat cgccagactt aaatgagtcg gtgtgaatcc
    42181 cattagcgtt accgtttcgc ggtgcttctt cagtacgcta cggcaaatgt catcgacgtt
    42241 tttatccgga aactgctgtc tggctttttt gatttcagaa ttagcctgac gggcaatgct
    42301 gcgaagggcg ttttcctgct gaggtgtcat tgaacaagtc ccatgtcggc aagcataagc
    42361 acacagaata tgaagcctgc tgccagaaaa atgcattctg ttgttgtcat gccgggtctc
    42421 tctcgtttgc ttctgctttc gccgccatca tttccagctt ttgtgaaagg gatgtggcta
    42481 acgtatgaaa ttcttcgtct gtttctactg gtattggcac aaacctgact ccaatttgag
    42541 cgaggctatg tgccatcccg atactcgttc ttaattcaac aggagatgct ttgtgcatac
    42601 agcccctcgt ttattattta tctcttcagc cagccgctgt gctttcagtg gatttcggat
    42661 aacagaaagg ccgggaaata cccagcctcg ctttgtaacg gagtagacga aagtgatcgt
    42721 gcctacccgg atattatcgt gaggatgctt cattaccatt gctccccata tacaaaacca
    42781 atttcagcca gtgcctcgtc cattttttcg atgaactccg gcaccatctc gtcaaaactc
    42841 gccatgtact tttcattccg ctcaatcacg acataatgca ggccttcacg cttcatgcgc
    42901 gggtcatagt tggcaaagta ccaggcatct tttcgtgtca cccacatgct gtactgcacc
    42961 tgggccatgt aagccgattt tatggcctcg aaaccaccga gccggaactt catgaaatcc
    43021 cgggaggtaa acgggcattt cagctcaagg ccgttgccgt cactgcataa accatcggga
    43081 gagcaggcgg tgcgcatact ttcgtcgcga tagatgatcg gggattcagt aacattcacg
    43141 ccggaagtga actcaaagag ggttctggcg tcgttctcgt actgttttcc ccaggccaga
    43201 gccttagcgt taacttccgg agccacaccg gtgcaaacct cagccagcag ggtgtggaag
    43261 taggacattt tcatgtcagg ccacttcttt cctgatcggg gttttgctat tacgttgtga
    43321 atttctgaag ctgtgatgac gccgagccgt aatttgtgcc acgcatcatc tccctgttcg
    43381 acagctctca cgtcgatccc agtacgctgc aggataatgt ccggtgtcat gctgccacct
    43441 tctgttcagt ggctttttgt ttcaggaatc caagagcttt tactgcttcg gcctgtgtca
    43501 gttctgacga tgcacgaatg tcgcggcgaa atatctggga acagagcggc aataagtcgt
    43561 catcccatgt tttatccagg gcgatcagca gagtgttaat ttcctgcatg gtttcatcgt
    43621 taaccggagt gatgtcgcgt tccggctgac gttctgcagt gtatgcggta ttttcgacaa
    43681 tgcgctcggc ttcatccttg tcatagatac cagcaaatcc gaaggcgaga cgggcacact
    43741 gaatcatggc tttatgacgt aacatccgtt tgggatgcga ctgccacggc cccgtgattt
    43801 ctctgccttc gcgggttttg aatggttcgc ggcggcattc atccatccac tcggtaacgc
    43861 agatcggatg attacggtcc ttgcggtaaa tccggcatgt gcaggattca ttgtcctgct
    43921 caaagtccat gccatcaaac tgctggtttt cgttgatgat gcgggaccag ccatcaacgc
    43981 ccaccaccgg aacgatgccg ttctgcttat cagggaaggc gtaaatttct ttcgtccacg
    44041 gattaaggcc gtactggttg gcgacgatca gcaatgcgat gaactgcgca tcgctggcat
    44101 cacctttaaa tgccgtctgg cgaagagtgg tgatcagttc ctgtgggtcg acagaatcca
    44161 tgccgacacg ttcagccagc ttcccagcca gcgttgcgag tgctgtactc atccgtttta
    44221 tacctctgaa tcaatatcaa cctggtggtg agcaatggtt tcaaccatgt accggatgtg
    44281 ttctgccatg cg
//
LOCUS       DAZFJF010000024        43639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFJF010000024 DAZFJF010000000
VERSION     DAZFJF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.024"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(179..337)
                     /locus_tag="ACHKAU_001496"
     CDS             complement(179..337)
                     /locus_tag="ACHKAU_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530591.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
     gene            complement(942..1017)
                     /locus_tag="ACHKAU_001497"
     tRNA            complement(942..1017)
                     /locus_tag="ACHKAU_001497"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(982..984),aa:Thr,seq:cgt)
     gene            complement(1132..2385)
                     /gene="proA"
                     /locus_tag="ACHKAU_001498"
     CDS             complement(1132..2385)
                     /gene="proA"
                     /locus_tag="ACHKAU_001498"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5530592.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2397..3500)
                     /gene="proB"
                     /locus_tag="ACHKAU_001499"
     CDS             complement(2397..3500)
                     /gene="proB"
                     /locus_tag="ACHKAU_001499"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5530593.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            3788..4843
                     /gene="phoE"
                     /locus_tag="ACHKAU_001500"
     CDS             3788..4843
                     /gene="phoE"
                     /locus_tag="ACHKAU_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5530594.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(4882..5283)
                     /gene="crl"
                     /locus_tag="ACHKAU_001501"
     CDS             complement(4882..5283)
                     /gene="crl"
                     /locus_tag="ACHKAU_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5530595.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5341..6585)
                     /gene="frsA"
                     /locus_tag="ACHKAU_001502"
     CDS             complement(5341..6585)
                     /gene="frsA"
                     /locus_tag="ACHKAU_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5530596.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(6677..7135)
                     /gene="gpt"
                     /locus_tag="ACHKAU_001503"
     CDS             complement(6677..7135)
                     /gene="gpt"
                     /locus_tag="ACHKAU_001503"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5530597.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7396..8853
                     /gene="pepD"
                     /locus_tag="ACHKAU_001504"
     CDS             7396..8853
                     /gene="pepD"
                     /locus_tag="ACHKAU_001504"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5530598.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(8910..9524)
                     /gene="prfH"
                     /locus_tag="ACHKAU_001505"
     CDS             complement(8910..9524)
                     /gene="prfH"
                     /locus_tag="ACHKAU_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO5530599.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9521..10660)
                     /locus_tag="ACHKAU_001506"
     CDS             complement(9521..10660)
                     /locus_tag="ACHKAU_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO5530600.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(10906..11358)
                     /locus_tag="ACHKAU_001507"
     CDS             complement(10906..11358)
                     /locus_tag="ACHKAU_001507"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5530601.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(11355..12410)
                     /gene="dinB"
                     /locus_tag="ACHKAU_001508"
     CDS             complement(11355..12410)
                     /gene="dinB"
                     /locus_tag="ACHKAU_001508"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5530602.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12481..13404)
                     /gene="lafU"
                     /locus_tag="ACHKAU_001509"
     CDS             complement(12481..13404)
                     /gene="lafU"
                     /locus_tag="ACHKAU_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5530603.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(13407..14270)
                     /gene="motA"
                     /locus_tag="ACHKAU_001510"
     CDS             complement(13407..14270)
                     /gene="motA"
                     /locus_tag="ACHKAU_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5530604.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(14283..14999)
                     /locus_tag="ACHKAU_001511"
     CDS             complement(14283..14999)
                     /locus_tag="ACHKAU_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO5530605.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(15019..15486)
                     /locus_tag="ACHKAU_001512"
     CDS             complement(15019..15486)
                     /locus_tag="ACHKAU_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO5530606.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(15494..16555)
                     /locus_tag="ACHKAU_001513"
     CDS             complement(15494..16555)
                     /locus_tag="ACHKAU_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5530607.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(16552..16863)
                     /locus_tag="ACHKAU_001514"
     CDS             complement(16552..16863)
                     /locus_tag="ACHKAU_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO5530608.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(16868..17260)
                     /gene="fliS"
                     /locus_tag="ACHKAU_001515"
     CDS             complement(16868..17260)
                     /gene="fliS"
                     /locus_tag="ACHKAU_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5530609.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(17283..18599)
                     /gene="fliD"
                     /locus_tag="ACHKAU_001516"
     CDS             complement(17283..18599)
                     /gene="fliD"
                     /locus_tag="ACHKAU_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5530610.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(18806..19720)
                     /gene="lafA"
                     /locus_tag="ACHKAU_001517"
     CDS             complement(18806..19720)
                     /gene="lafA"
                     /locus_tag="ACHKAU_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO5530611.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            20206..21048
                     /locus_tag="ACHKAU_001518"
     CDS             20206..21048
                     /locus_tag="ACHKAU_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO5530612.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(21103..22080)
                     /locus_tag="ACHKAU_001519"
     CDS             complement(21103..22080)
                     /locus_tag="ACHKAU_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO5530613.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(22097..23026)
                     /gene="flgL"
                     /locus_tag="ACHKAU_001520"
     CDS             complement(22097..23026)
                     /gene="flgL"
                     /locus_tag="ACHKAU_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5530614.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(23041..24417)
                     /gene="flgK"
                     /locus_tag="ACHKAU_001521"
     CDS             complement(23041..24417)
                     /gene="flgK"
                     /locus_tag="ACHKAU_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5530615.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(24671..24970)
                     /locus_tag="ACHKAU_001522"
     CDS             complement(24671..24970)
                     /locus_tag="ACHKAU_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO5530616.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(24970..26070)
                     /locus_tag="ACHKAU_001523"
     CDS             complement(24970..26070)
                     /locus_tag="ACHKAU_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5530617.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(26085..26750)
                     /gene="flgH"
                     /locus_tag="ACHKAU_001524"
     CDS             complement(26085..26750)
                     /gene="flgH"
                     /locus_tag="ACHKAU_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5530618.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(26833..27618)
                     /gene="flgG"
                     /locus_tag="ACHKAU_001525"
     CDS             complement(26833..27618)
                     /gene="flgG"
                     /locus_tag="ACHKAU_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5530619.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(27697..28434)
                     /locus_tag="ACHKAU_001526"
     CDS             complement(27697..28434)
                     /locus_tag="ACHKAU_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO5530620.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(28434..29636)
                     /gene="flgE"
                     /locus_tag="ACHKAU_001527"
     CDS             complement(28434..29636)
                     /gene="flgE"
                     /locus_tag="ACHKAU_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5530621.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(29660..30373)
                     /locus_tag="ACHKAU_001528"
     CDS             complement(29660..30373)
                     /locus_tag="ACHKAU_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO5530622.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(30373..30804)
                     /gene="flgC"
                     /locus_tag="ACHKAU_001529"
     CDS             complement(30373..30804)
                     /gene="flgC"
                     /locus_tag="ACHKAU_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5530623.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(30807..31142)
                     /locus_tag="ACHKAU_001530"
     CDS             complement(30807..31142)
                     /locus_tag="ACHKAU_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO5530624.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            31224..31961
                     /gene="flgA"
                     /locus_tag="ACHKAU_001531"
     CDS             31224..31961
                     /gene="flgA"
                     /locus_tag="ACHKAU_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5530625.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            32042..32320
                     /gene="flgM"
                     /locus_tag="ACHKAU_001532"
     CDS             32042..32320
                     /gene="flgM"
                     /locus_tag="ACHKAU_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5530626.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            32333..32761
                     /gene="flgN"
                     /locus_tag="ACHKAU_001533"
     CDS             32333..32761
                     /gene="flgN"
                     /locus_tag="ACHKAU_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO5530627.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            32888..33817
                     /locus_tag="ACHKAU_001534"
     CDS             32888..33817
                     /locus_tag="ACHKAU_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO5530628.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            33996..36440
                     /locus_tag="ACHKAU_001535"
     CDS             33996..36440
                     /locus_tag="ACHKAU_001535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5530629.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(36558..36953)
                     /locus_tag="ACHKAU_001536"
     CDS             complement(36558..36953)
                     /locus_tag="ACHKAU_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO5530630.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(36956..37390)
                     /gene="fliJ"
                     /locus_tag="ACHKAU_001537"
     CDS             complement(36956..37390)
                     /gene="fliJ"
                     /locus_tag="ACHKAU_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5530631.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKIIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(37393..38706)
                     /gene="fliI"
                     /locus_tag="ACHKAU_001538"
     CDS             complement(37393..38706)
                     /gene="fliI"
                     /locus_tag="ACHKAU_001538"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5530632.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESCL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSIVVVAPADE
                     SPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEPPATKGYPP
                     SAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGHIVLTRKLA
                     EAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLGGYVAGADA
                     SVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(38699..39409)
                     /gene="fliH"
                     /locus_tag="ACHKAU_001539"
     CDS             complement(38699..39409)
                     /gene="fliH"
                     /locus_tag="ACHKAU_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5530633.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(39412..40422)
                     /locus_tag="ACHKAU_001540"
     CDS             complement(39412..40422)
                     /locus_tag="ACHKAU_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5530634.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(40400..42046)
                     /gene="fliF"
                     /locus_tag="ACHKAU_001541"
     CDS             complement(40400..42046)
                     /gene="fliF"
                     /locus_tag="ACHKAU_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5530635.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(42051..42392)
                     /locus_tag="ACHKAU_001542"
     CDS             complement(42051..42392)
                     /locus_tag="ACHKAU_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5530636.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(42407..43393)
                     /locus_tag="ACHKAU_001543"
     CDS             complement(42407..43393)
                     /locus_tag="ACHKAU_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO5530637.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
BASE COUNT         9564 a        12123 c        11397 g        10555 t
ORIGIN      
        1 agccttaaat accccaataa ttaattttaa ggttttccgt gttctcttct gcattatgaa
       61 aatgcaaaat aaggcacaaa acaagctcac atacgcatta aacacacatc acctatacat
      121 ccctatgcca agcaatgaaa acagcgttag aatgcgtcct gttgcgttct ggagattttc
      181 agaaatcctc tctaagccat ttcaactcac taaggagttt tctttcttcg ttctctcgtt
      241 gctttcgctc tagccaggct tttccctctg gcgtagacag gaacttacga gcgtgaatct
      301 tgcggttatt acgtttgata gctgcaatgt ttttcatcgt gtgcctctgt attcatatga
      361 aaattaacaa gttacatact acacagggag actacgcata aaacacaata caagtaaaga
      421 tacattttca agacaatagg agccataaag cgcctttgat ggttatacca aaaaataagc
      481 gccgtaggcg tatccactaa aaagggcttt ttcacaaaag ctatgaagtc ttagcagttc
      541 ttctcttatc aggcacccgt tgtactccgg tggacgaaac cgtgctagag cttgattctt
      601 tcccgatcaa gttaggtttt gaagaggtgg gctttaatcc actatgcact taaaactctt
      661 cccatcattt agcctgttta tcctctgcaa cgtctcacga cgtgtagcat cattggacac
      721 cgcgccaggt gtggaacatc ttgctaagag ttttggtaga aggtttagag acggtggcac
      781 gtcttccagc ttcaaaggaa cttgttacga ggttgagcct gggaactact cgcccatacg
      841 tgttacacta ccaaagccca taacaacgta taggcgtttg taagtgataa cagaagatac
      901 aatttcttag agaagttaag ctaagttatt gattttctta atggtgccga taataggagt
      961 cgaacctacg accttcgcat tacgaatgcg ctgctctacc aactgagcta tatcggccct
     1021 gaaaggacat gttcacgaac gtgaatcacg gtggacaagg ttaaaactaa ccgggcgatg
     1081 cgtcaatggc cttgtgaatc aaatggctac ttttgcatca cccggtttta tttacgcacg
     1141 aatggtgtaa tcaccaatgc cgatccactt gtaagtggtc agtgcttcca gccccattgg
     1201 gccacgcgcg tggagttttt gtgtgcttac cgccacttcc gcacccagac caaactggcc
     1261 gccgtcggta aaacgcgtag aggcgttaac gtaaacagcg gacgaatcca cttcgttaac
     1321 aaaacgctgg gcgttgcgca tatcgcgggt caggatcgca tcggagtgtt gtgtgccgtg
     1381 ttcacgaata tgggcgatgg catcgtcaag atcgctgacg attttgacgt tcaaatctaa
     1441 tgacagaaac tcatcgtcat actcttcggc tttaacagca accaccttcg cagggcctgc
     1501 ctgcaactgc gccagtgcag ctgcatctgc gtgtaatgtc acgccgcttt ccgccatttg
     1561 tttgcttaat gcgggcagga agctatcggc gatgttttta ttcaccagca acgtttcaac
     1621 cgtattacat gtgctcggac gctgagtttt cgcgttgacg atcactttta atgcttcagc
     1681 gatctctaca ctttcatcaa cgtaaatatg gcatacgcct ataccacctg tgatcaccgg
     1741 gattgtcgac tgttcacggc acagtttatg caaaccagcg ccaccacgcg ggatcagcat
     1801 gtcgatgtat ttatccatac gcagcatttc actgaccagc gcacggtcag gattatcaat
     1861 cgcctgcacg gcacccgccg gtaagccgca ggatttcagg gcgtcctgaa tcaccgccac
     1921 cgttgcagcg ttagtgcgac acgtttcttt gccaccgcgc aggatcaccg cattaccggt
     1981 tttcaggcac agcgaagcga catcaaccgt cacgttcggg cgcgcttcat aaatcacgcc
     2041 aataaccccc agcggtacgc gacgacgctc aagacgcagg ccgctgtcca gtacgctgcc
     2101 atcgattacc tgccccaccg gatcggcgag gttacacacc tggcgcacat catcggcaat
     2161 gcctttcagc cgtgcgggcg tcagtgccag acggtcaagc atcgcttcgc caaggccatt
     2221 ggcacgcgcg tcagcaacat cctgggcgtt agcgttgagg atgatttcgc tttgtgcttc
     2281 cagttcatcg gcgatttttt ccagcacgcg atttttttcg cggctggaga gttgcgctaa
     2341 tttatacgag gcttgcttcg cggcaatgcc catttgttcc agcatcagcc tgctccttaa
     2401 cgggtaatca tgtcatcacg gtgaacggca accgggccgt attcatatcc cagtattgca
     2461 tcaatttctt gcgagtggtg cccggcaata cggcgtaatg catcgctgtt gtaacgactg
     2521 acgccgtggg cgatatcgcg accttcgagg ttgcaaatgc ggatgacttc accacgcgag
     2581 aaattgccag tcacgctttt aatgcctttc ggcaacaggg agctgccgcg ttcaagaatg
     2641 gcggcagttg ccccttcatc taccgtgatt tcacccgccg gcggcgcacc gaaaatccag
     2701 cgtttacggt tttcaagcgg agtcgcctgg gcatggaaca gcgtaccgac ggaaatgcct
     2761 tccatcacat caccaataac gcccggcttg ctgcccgcgg caataatggt gtcgataccc
     2821 gcacggcaag ccacgtcagc agcctgcaat ttggtactca tgccgccagt tccgaggcct
     2881 gaaacgctgt caccggcaat cgcgcgcagt gcgtcatcaa tgccgtaaac atctttaatc
     2941 agttctgcct gcggattgct gcgcgggtca gcggtataca aacctttttg atcggtcagc
     3001 agcaacagtt tatcggcacc cgccagaatc gccgccagcg cagaaaggtt atcgttatcg
     3061 ccgactttaa tctctgccgt agcgacagca tcgttctcat tgattaccgg aacgatattg
     3121 ttatcgagca acgcacgcag ggtgtcgcgg gcgttcagga agcgttcacg gtcttccata
     3181 tcagcacggg tcagcagcat ttgcccgacg tgaatgccat aaatcgaaaa cagctgttcc
     3241 cacagttgaa tcagtcgact ctgccctacc gctgccagca gttgtttcga ggcgatagtc
     3301 gctggcagtt ccgggtaacc caggtgctca cgtccggcgg cgatcgcgcc cgacgtcaca
     3361 ataacaatcc gatgcccggc ggcatgtaac tgcgcgcact ggcgaacaag ttcaacgata
     3421 tgggcacggt tcagacggcg cgatccgcct gttagcacac tggtgccgag ttttaccacc
     3481 agcgtctggc tgtcactcat gattctctgc cattcaattt taggaaaaat gatatcaaac
     3541 gaacgtttta gcaggactgt cgtcggttgc caaccatctg cgagcaaagc atggcgtttt
     3601 gttgcgcggg atcagcaagc ctagcggcag ttgtttacgc ttttattaca gatttaataa
     3661 attaccacat tttaagaata ttattaatct gtaatatatc tttaacaatc tcaggttaaa
     3721 aactttcctg ttttcaacgg ggctctcccg ctgaatattc gcgcgttaat taaaatcagg
     3781 aatgaaaatg aaaaagagca ctctggcatt agtggtgatg ggcattgtgg catctgcatc
     3841 tgtacaggct gcagaaatat ataataaaga cggtaataaa ctggatgtct atggcaaagt
     3901 taaagccatg cattatatga gtgataacgc cagtaaagat ggcgaccaga gttatatccg
     3961 ttttggtttt aaaggtgaaa cacaaattaa cgatcaactg actggttatg gtcggtggga
     4021 agcggagttt gccggaaata aagcggagag tgataccgca cagcaaaaaa cgcgtctcgc
     4081 ttttgccggg ttgaaatata aagatttggg ttctttcgat tatggtcgta acctgggcgc
     4141 gttgtatgac gtggaagcct ggaccgatat gttcccggaa tttggtggcg attcctcggc
     4201 gcagaccgac aactttatga ccaaacgcgc cagcggtctg gcgacgtatc ggaacaccga
     4261 cttcttcggc gttatcgatg gcctgaactt aaccctgcaa tatcaaggga aaaacgaaaa
     4321 ccgcgacgtt aaaaagcaaa acggcgatgg cttcggcacg tcattgacat atgactttgg
     4381 cggcagcgat ttcgccatta gtggggccta taccaactca gatcgcacca acgagcagaa
     4441 cctgcaaagc cgtggcacag gcaagcgtgc agaagcatgg gctacaggtc tgaaatacga
     4501 tgccaataat ttttatctgg caacttttta ttctgaaaca cgcaaaatga cgccaataac
     4561 tggcggcttt gccaataaga cacagaactt tgaagcggtc gctcaatacc agtttgactt
     4621 tggtctgcgt ccatcgctgg gttatgtctt atcgaaaggg aaagatattg aaggtatcgg
     4681 tgatgaagat ctggtcaatt atatcgatgt cggggctaca tattatttca acaaaaatat
     4741 gtcagcgttt gttgattata aaatcaacca actggatagc gataacaaat tgaatattaa
     4801 taatgatgat attgtcgcgg ttggcatgac ctatcagttt taatgattat tgccggatgt
     4861 gatgcatccg gcacatttca ctcacgccgt taacttcacc ggctcgtcac gaaaatcatc
     4921 cgccggttcc agcttcagat tcagcgtcgt cagcagttca cgcagcttct cgtgaaactc
     4981 acgcagggtg tgctccagtc gttcaaccac ttcagtgtct tttaccggaa cactcttcca
     5041 gtcgcctgct ttatcgaaca gaccaaactg gtaactgtag gtaaaacggg attcctgcgc
     5101 ttcaagctcc atccaccagc cccagaactc acgcacttcc ggtgccggtt tcacgttgac
     5161 gcatacagcc agacaatcga aaaagaatcg attatcttcg cacttacctt cacgaatata
     5221 cgggcctagt gcggtaaatt ttttgatcaa tctgctcttc gggtgtccac tcggtaacgt
     5281 cattgctatc tcctgttgtg atgcaactgt tttaccaaat tggcaaaatt tagcaaattt
     5341 ttaacacaag cgtttttcga tccagccggt gatttcctga agacctttgt caaaattccg
     5401 atacaccggg ttaaatggga tctctaataa tttaccgtca gcagatgatg aggtgattaa
     5461 gcgtgagtcc tcttccgggc tgaacggatc gttcttccag tagcctgata acattggcgt
     5521 tgggcagcga cgccccagca atccttgcac ttttaatgaa tagcgattca gctccacgcg
     5581 taacgcgtca tcggaagcat catgcatccc caaacgactc gccagaacgt caagatacat
     5641 ttccggcacc tgttgctggc acttaaaatc actcaacagg gtatgaacta ccggaccaag
     5701 acaggcaacc gctttcagac gcggcgattc aagatatgcc agacgcacgg caacgttagc
     5761 gccgaaacgg aaaccaaagg ccgcgacgcg agtgtgatcc acccacggta cgttaggcag
     5821 cgcctttaag acgtgctgat gcaacaggct ggagtcctgg gtgagcttcc attttgaaga
     5881 aaagcccacc gatggcatat caatagtcag catcgcaatg ccgcgcggcg caaaataacg
     5941 ttcatacagg ctgtaatagt ccgtctgcat cgcatccaga ccaccacaca ttaatactgt
     6001 cgggaacggg ccatcgcctt tcggcatatg caaaaagccg gtgatgggcg caccgccggg
     6061 tacggtaaac tccatctgcc gcatcgtgcc cggcagacgc tgggcggcct cttcataggc
     6121 gcggtttgat aaagcctgcg cttgctcggc caggtcatct cctttcagat gaggataggc
     6181 ggcaatgttg tacaacgtag ccgcatgcag ccagtgtcga ccactgagtt gcggatcttc
     6241 ttccgcacag gctttttgtt gccacaccat cgcctgggtg gcccactcat aaatccagtt
     6301 gccaccacgg tagccaatca cagtatcgta taaatcatcg tcggtacgtt cagcatcgct
     6361 catcacaata cgtgcctgga cgtcgaggat ttcacgtggg tcaataccgc gccagatcca
     6421 catcagacgg ttaatcatgc gataccagtg agggatggtt ttaccatcaa gagccgactg
     6481 cacaggcggt tgtgcgccgt gattaaagcg gcggactagc gtcgaggttt caggatgttt
     6541 aaagcggggt ttgaacaggg tttcgctcag gtttgcctgt gtcatggatg cagcctccag
     6601 aatacttact ggaaactatt gtaacctgcc tgaagttaaa aagaacaacg cccggcagtg
     6661 ccaggcgttg aaaagattag cgaccggaga ttggcgggac gaatacgacg cccatatccc
     6721 acggctgttc gatccaggta tcttgcggga tatcaacaac atagtcatca accagcggac
     6781 gaccagccgg ttttgcgaag atggtgacaa agtgcgcttt tggatacatt tcacgaatcg
     6841 caacagcagt accaccggta tccaccaggt catcaataac gatgaagcct tcgccatcgc
     6901 cttctgcgcg tttcagcact ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac
     6961 aaacggtatc gacatgacga atacccagtt cacgcgccag taacgcaccc ggtaccagac
     7021 cgccacggct tacggcaata atgcctttcc attgttcaga aggcatcagt cggcttgcga
     7081 gtttacgtgc atggatctgc aacatgtccc aggtgacgat gtatttttcg ctcatgtgaa
     7141 gtgtcccagc ctgtttatct acggcttaaa aagtgttcga ggggaaaata ggttgcgcga
     7201 gattatagag atctggcgca ctaaaaacca gtatttcaca tgagtccgcg tctttttacg
     7261 cactgcctct ccctgacgcg ggataaagtg gtattctcaa acatatctcg caagcctgtc
     7321 ttgtgttgac aacattttct gctaaccctg tgacctgcaa tactgttttg cgggtgatcg
     7381 acaaggagac ttaacgtgtc tgaactgtct caattatctc cacagccgct gtgggatatt
     7441 tttgccaaaa tctgttctat tcctcacccg tcctatcatg aagagcaact cgctgaatac
     7501 attgttggtt gggcaaaaga gaaaggtttc catgtcgaac gcgatcaggt aggtaatatc
     7561 ctgattcgta aacctgctac cgcaggtatg gaaaatcgta aaccggtcgt cttacaggcc
     7621 cacctcgata tggtgccgca gaaaaataac gacaccgtgc atgacttcac gaaagatcct
     7681 atccagcctt atattgatgg cgaatgggtt aaagcgcgcg gcaccacgct gggtgcagat
     7741 aacggcattg gtatggcgtc tgcgctggcg gttctggctg acgaaaacgt ggttcacggc
     7801 ccgctggaag tgctgctgac catgaccgaa gaagccggta tggacggtgc gttcggctta
     7861 cagagcaact ggttgcaggc tgatattctg attaacaccg actccgaaga agaaggtgaa
     7921 atctacatgg gttgtgcggg gggtatcgac ttcacctcca acctgcattt agatcgtgaa
     7981 gcggttccag ctggttttga aaccttcaag ttaaccttaa aaggtctgaa aggcggtcac
     8041 tccggcggtg aaatccacgt tggcctgggt aatgccaaca aactactggt gcgcttcctg
     8101 gcgggtcatg cggaagaact ggatctgcgc cttatcgatt tcaacggcgg cacactgcgt
     8161 aacgccatcc cgcgtgaagc ctttgcgacc attgctgtcg cagctgataa agtcgacgtc
     8221 ctgaaatctc tggtgaatac ctatcaggag atcctgaaaa acgagctggc agagaaagag
     8281 aaaaatctgg ccttgttgct ggactctgta gcgaacgata aagctgccct gattgcgaaa
     8341 tctcgcgata cctttattcg tctgctgaac gccaccccga acggtgtgat ccgcaattcc
     8401 gacgtggcaa aaggtgtggt cgaaacctcc ctgaacgtcg gtgtggtgac catgactgac
     8461 aataacgtag aaattcactg cctgatccgt tcactgatcg acagcggtaa agactacgtg
     8521 gtgagcatgc tggattcgct gggtaaacta gctggcgcga aaaccgaagc gaaaggcgca
     8581 tatcctggct ggcagccgga cgctaattct ccggtgatgc atctggtacg tgaaacctat
     8641 cagcgtctgt tcaacaaaac accgaacatc cagattatcc acgcgggcct ggaatgtggt
     8701 ctgttcaaaa aaccgtatcc ggaaatggac atggtttcta tcgggccaac tatcaccggt
     8761 ccacactctc cggatgagca agttcacatc gaaagcgtag gtcattactg gacgctgctg
     8821 actgaactgc tgaaagaaat tccggcgaag taattatttg atttgctgcc ggatggcgtt
     8881 taatcgcctt ccggcagttt catccttcat tatccttcga taaaagccat ccctgtaaat
     8941 gtccgtctcg ggttgccacg ttcaatctgg tgatggaaca ttcgccgcag cgatttcagc
     9001 accgcgctat tttcctgttg ctgctgctcc agcttccagg caatcagcaa tcgtgccagc
     9061 cgcttgttag catgctgact acgctctgac tgaaccttca cgctaatacc ggatgccaaa
     9121 tgcgtggcgc gtaccgccga gtcggtttta ttgacatgtt gaccgcccgg ccccgacgaa
     9181 cgcaacgtct cataacggat tgcatccgat tgttcctgct catcagcggt aaaacgccca
     9241 atgcccagaa accagttttt gcgcccatga tgaggccgat acggactcgg acaaatccac
     9301 tgaatagtgc cacaccagct ttcgcttaat gcccatgcgt tatcgccatc cagagaaatc
     9361 agcgccgaac gcagcgtgtc agagtagtgg cccgtttctg tttccagcac cgttaccgcg
     9421 acgtcttgtc gggcagcttc tttaattagc ctgtccagcg cctttttcac tgcgagacaa
     9481 cattcttccg gcccctgcgc tgaggagagt tgtagcaaga tcatcctttt ttccctccac
     9541 tgtttttgag cgttagcacc ggacgcagcc gcgcgacagg aataattaaa ccggcctgca
     9601 ccagacattg caccacgctt tcagccgatt tataggcttg tggcgcttct tcaaagatga
     9661 gttgtttatc gcgacaaatt acccgactgc caagttcagt acgcgagagt tgcgtcgccg
     9721 tgtatttcgc tgccagacgc cctttacact cggtgcgccc ccatttacgc cctgccccat
     9781 gcgccagcga gtggagcgtt ttttcgttcg caacaggctg tacaatccag gagtaatcac
     9841 cacgtgaacc gggaataatc accagaccgt tgtcatccgg cgtggcacct ttacgatgca
     9901 accagccctg ttgatcgccg atttgacacg cgctaacaaa gttatgcgcc acatccagaa
     9961 ccggacttcc cgtggcctta acttgttgca ttatgcgcaa agcgatcatc tggcgattaa
    10021 tacacgcaaa cgccagcgca tcgtcatgtt ccgcaatata ccgtagcgcg tcgtcactgc
    10081 cttgaggcaa accatgatgc gagaacgagg caatatgccg ctgtaaaata gactgcccca
    10141 ccccccgcga gccgctatga accaacagtt gcagatgttg tgcatccaga cctgccagtg
    10201 caaacagttc agcgttgatg atttgatcaa cttgttgcag ttcagcgaaa tgattgccgc
    10261 caccgataga gccaagcgag ctgcgccacg gatgctgtgc aaatgctgac ggcaggtttt
    10321 cctccagcca gctttcttca gcaacgtcat ccagatctga taatcgcttt tcaaacttat
    10381 cggcgttgta tttgcgagcg agaatatctg tttgccatag cgccataccg cagccgatat
    10441 cattgccgac cagtgccgga taaaaacgac caacagagaa gaacgctgcg ccaatcggat
    10501 agccgcgtcc gggatgtaaa tctggcatcc ccactacgcg ctgcatgttg ggtaaatttg
    10561 ccgtggtgtg taattgttgg atcgctaaac tctcgatcca caggtcatca gatgcgatgg
    10621 taaataccgc atcggataag ggacgaatat aattgcccat ataccattcc gtaaatagac
    10681 tcgtcatact tcaagttgca tgtgttgcga cttgaattat tgagagtaac aattaaaaaa
    10741 tcaggaaatg gcagacgaat ccagggcaag aaaaaaagaa ttaatcctgg aaaggtctgc
    10801 aaagagtcat tgataaagga tttgtcatga tttacctcct tgcagtttgt ggaataagaa
    10861 aacgcgcaca tactaggctt gcgttatttt cattgcaagc tggatttaat gttgctgttt
    10921 ataacgcata taaaaattaa taaaccactc tccccgacat tcaacgcgtt gctgcttaac
    10981 tgtctgaaaa ccataacgtt caaaaaaggg ttttgcggtt atgcttgcgt ccaccgtaag
    11041 ttcggattca gacttaatca aaggttttaa caaagcgctg gcaaccccac ggcgggtgta
    11101 ttcagggtca acaaataaca tatcgatata gtgttcaacg caggtaataa agccgaccag
    11161 ttttgcatta atgactgcaa cccgcacttg tgatttcgcg agtttctcct tccagcgaga
    11221 ttcgtcaatc tgcgcccagg cggcaatttg ttgtggtgaa taatgctgac tggcagtcat
    11281 cgtaaccgct ctaatgaaaa tagcgcatag ttgctgaaaa tcgccaggct gatagtctct
    11341 tatttgtatg ttattcataa tcccagcacc agttgtcttt ccatttgcgg gtcaagcaac
    11401 gtcacatgca gccccaccag tcgcacaccg cgcccgccgc ggcgttcatc ccaggtttta
    11461 cgcgcggtgg cgattaagtc agctttattc agccgcggcc agacgtgctc ctgagtggtt
    11521 tgctgaaaat catcaaactt taatttcact ccctgacggg cgatcagtaa atcaggtttt
    11581 acctttgcca gacggcgttc aagttccgga tacagccgct cgataatcgc ttcacattca
    11641 gaccagtgat gaatatcttc cgccatcgtg cgttccacgc cgacggattt tcgtaaccgt
    11701 tcgctgttaa cgtcgcgctc gtcaatcccc tgactacgct cccacaaaat gcggccaaat
    11761 ttgccaaagc gtttaagcag catcaccaga tcacactttt gtacatcacc gcaggtccgc
    11821 agccccatcg cttccagttt tgccgccgag actttgccga cgccggggat ttttgccagc
    11881 ggtaaggttt gtaaaaatgc cggaacttct gccggcgtaa tcacaaactg accgttgggt
    11941 ttattcatgt cggaggcgat tttggcgaga aactttactg gtgccacgcc cgcagacgct
    12001 gtcagttgca gctcgctgaa aatcgtctgg cggatttcct gggcgatgag ggtcgcagaa
    12061 ccgtggcaat ggacgctatc ggtgacgtcg agataagcct catccagtga caacggttca
    12121 atacgcgagg tgtagcgcga gaagatttca cggatatgat ttgaggcttc tttgtaggcg
    12181 tcaaagcgcc ccggaagcaa ggtgagatgc gggcataatt tgagcgccat ccctgtcggc
    12241 atagcgctac gtacgccaaa tttacgcgcg ggataattgg cggtgctgat caccccccga
    12301 cgttcgcggc tgccgccaat agcaataggg atatcgcgca gggcgggatt gtcgcgcatc
    12361 tccaccgctg cgaaaaagca gtccatatcc acatgaatga ttttacgcat tactcacctc
    12421 tcaacactgg taaagtatac agtgatttca gggtttgaga aatgcgtaaa gattcagcat
    12481 ttaacgcgtt cgctgcgaaa gcacctgctg cttatccagc ttttgcacca gcggctgcac
    12541 cactttatcc ccatgctgac caaagtattg atacagcgta tcggacgcac ttttggtcag
    12601 caccataatc tcaatgcgcc ggttgcccgc gctttgcgga tttttggaat ccagcagcat
    12661 ctggtccgcc attgcgctta cctgcatcac tttatcttcc ggcattccgg cctcttccag
    12721 cacccgacga gccgaaagcg cgcggtcacc cgaaaggttc cagttgttgt agatattgtt
    12781 tttgtaagcc atcgcatcgg tatgcccggt aataataatt ttgttatcga gcgagtcgaa
    12841 aactggcgcg agctccacca gcagcgtttt aaagaacggc ataatctgcg cgctgccgcg
    12901 ttcgaacata ttgcgattct ggtcgtcttt aatcagtacg cgtaatccct ggggaacaat
    12961 ctccatctcc agattcgctt ccatatgcgc atcacgggca atggtgttga tactggttgc
    13021 cagctcgcca agctcggtgg cggatttctt cttcagcagc gccgttttct cattcacatc
    13081 acgcgctttt ttctccgtct cttccggcac cgccacggtg gcaggttttg gcggatgaga
    13141 cgggctgatt ttatttaacg gcgacagccc gccaccgtta aaaatcgact gcccgtgcag
    13201 cgcggcgata atgctttcac gctcggattt actgacgctg ttgacaatcc acagcgtcat
    13261 aaacagcgcc atcatcgcca gggtaaagtc ggcgaacgcc accttccacg ccccgccatg
    13321 atgcccggca tgattctttt tgatctgccg ccggacgatg gtggtttttg ccccacgctc
    13381 gcggcggttg cccgtctttc tcatgcttat tcttgctcca gcatcgcatt cacccacgcg
    13441 tcgaggttgg cgaaggtcgg tttggacgcc agatgcagca gcttacgccc ggcatccacc
    13501 gccagcagcg tcggtttgcc gcctgcctgc gccaccagca cagtgcgcac gcattccagc
    13561 aaagaatgtt ctgcccgcgc ctgttgttcc atggcgttgg cgagcgggtc catcaggcag
    13621 tagcagataa agacgcctaa aaacgtcccc accagcgctg ccgccacttt caggccgatc
    13681 agagcaatag agccgtcaat cgactgcatg gtgatgatga tccccagcac tgctgcacaa
    13741 ataccaaatc ccggcatcgc ttcggcggta cgttgcagcg agcgcgacgg cgtgagcagc
    13801 gactcttccg cagtgtccag ttcctgatcg agaatgcctt ccagttcatg ggcgtcgatt
    13861 ttacccatcg ccatcaggcg aaagttatcg gcaataaagg tcaccagccg cttctgggtt
    13921 aacaccagcg ggtatttctg gaaaatggtg ctctcttccg gttgctcaat atgttgatcc
    13981 agcatgcgca gacctccgtt ttgcaccatt tcgagcaact catacaggca cattaatagc
    14041 tgacgctgaa actccggccc gagctgtttt ttactgatca cacctttaat ttgatgggca
    14101 atctctttca gcacatgttt cgggttacca ataatcatcg ccccgaaacc ggcacccaaa
    14161 ataatgatca tctccgccgg ttgccagata gccactaatt gcccgcccgc ttcaaaataa
    14221 ccgccaaata cgcagccgag caccaccagt aaacctaata ttttttgcat gatttcttca
    14281 ctttaattcg gatagacaaa atcacgaatt ttctgcgcga tctgtttatt cagttgacaa
    14341 attcgggcct cggttagccc taataccagc gcaatctctt tgagattcat ctcatgctgg
    14401 taatacatgg agaggatcag ttgctctttt tcgcttaatt gtgaaagcgc ctgttgcagc
    14461 atttgctgag tgacaaactg atcttccagc tcccggccaa caagcggaat gcctggccct
    14521 tcgccgttca gcagttcgtc caggctggcg agcgtttccg ccccttccag ttgctgatat
    14581 tcgagataat cttcatgtga gatcccttcc acggccagtt ctgaccactc cggttcgtgc
    14641 cccagttttt tgcgcgtttc gcgaatcaga tctttcatct gatgatattt ctgtcgcagc
    14701 tggcgcggtc gccagtcgag agcgcgcagc tcatccagga tcgcgccgcg aatacggtgc
    14761 acggcatagc ccgcaaaacc ttcgtccggc agcccgtagc ggcggatggc attaagcagc
    14821 cccattaacg ccgtttgttc catatcctga cgatcaataa tgcaattaca ctgcggggca
    14881 agttgccgca ccactttacg caccaacggt aaataggcct gcaagtagtg gctctcctgc
    14941 tgcggggtta ataccggcgc ggcagcaaat tcttctgact cgaatacatc ctgtagcatg
    15001 tttatatccg cctggtgatt actggaagac cacttttttc accagcaaat ccttatacgg
    15061 tgcgctcatt ttgcgacgct gaagatcttt ctttaacgcg tcagaaatga gtttgcggat
    15121 ggcagatatt ttcatgccgc gcaaatcttc aaatttcatt tccgtcaggc attcaaccgc
    15181 aatactttga tacagcggct cctgatgttt tattttttcc gcttctttgt cactggcgac
    15241 caccatcact aattccgccg acaaataatg gtccgcgccg ttgttgtcat gcagcgtgac
    15301 aatggtttcc ggcaacgtca caaacacgct tttgctttca tccagggttt ccaccgtttc
    15361 cgtctgcgcg gcggcagtcg gttttccttt tccggcataa tgatgccaga caccccatcc
    15421 agctccggcc cccaccacca acgccagaac ggcgctgacc acgcccgcca ccacgatttt
    15481 cttcatattg cctttacact ttgatcaaaa cggattcgtt ctgttgaaat tgaggcgcat
    15541 catcaagctg taaggcggca ctcacctctt cctgctgatg gcccgactgt tgttgttgct
    15601 gcgactgccc ttcggaggaa acctgtacgt tcacctccat aaaattttgc gccgtcagat
    15661 gctggcggag atcgtcgcta aactgctgta acgcgcggca aacttcactt tggttcgcgc
    15721 cgatgtgcac catcagtttg ccgttgtcga gttgtacggc gatctcgagc ttgccaagcg
    15781 acggtggatc gagacggatg gtggagatct gctgttgttt gttgagctga aagtggatct
    15841 gatctttcaa aagcgtcgtc agtttttcgc ccagttcgtc catcgaaagc gtggctggtt
    15901 tggcgacaat ctcctgatga tcgctgagca gctccggcgt cttaacctgc aacaccgcac
    15961 gcgcaataac cggttcactg tccggcactt tttccgtttt acgctcgacc tgcgggcgcg
    16021 cggtcacacg ggttagttca gatttcgggc gagctggctg tgttgagacg cgcggctgcg
    16081 tcggcgtaat ggcatgtaaa tcttcactag cgtaagtcgc cagtctggcc tgctgttcgg
    16141 gcttcgcctg cggcaactga ctgatcagtt cctgcaactg cggcggcaac ggcgtgagtt
    16201 cctgctgttg cgtctggcgt tgcggcgcgt tttgcaccac cgctttcgtc aattgctgga
    16261 taaccggcga tgttgcgacg tttcgcggct gcggcgtgtc ctgatgcggc gcggcaggtt
    16321 gcggaagtaa cagcgccatt agcgcctgca tcgccaccgg atcgttctcc tgcgggcgcg
    16381 tcgcctgttg ctgactggtt tttgcgctat gaacgcgctc cgccaccgcc gcgaccgcca
    16441 ttttcggcag ggtaaacgcg ggcgcgtttt caccgctcac cggcggtaaa atatccgcct
    16501 tcaacgaagc ggtttccgca agcgttccga gcgtggcaag caatgtcgga ttcatgactt
    16561 ctccgaaagt aaattaacaa ttgagtaggc cagtacgcct tcctgatgcc gctgatgctg
    16621 ttccatacgc gctttcaaca cttctgcgcg ctgctcgcgc cgtttaatca gggcgttaaa
    16681 cgcgtcatac agtcgcgctt tcactgcatc caactgcgcc gcatcggttt ccgccgccgc
    16741 gtaatcgtga aactggcgta tcaactgctg atgcagtgcc ggaaggcgtc gccagcgttg
    16801 cttgtcgagg gcttcattca tcgcctcgac cagtgcgaaa agtttcctgc gttgtttttc
    16861 catcgtctta acccagcttg cccgaaagcc cttcccagcc ttcgcgcagg ttgcttaaga
    16921 tcagcatcac ttcatcgatt ttctctgccg acagttcgcc gctggcctca tacaatcggt
    16981 aaacgcagtg gtcatagagt cgcgaaaggt tgaccaccag ctcgccgcca gtttcaaact
    17041 ccagcgaact ggtgagcgca ttgagaatgt cgatgcattt attgatgctc tgggctttct
    17101 tctcgaaacg ccgcccttca atatggcttt tggcgcgttc cagttcgtcc atcaggccag
    17161 aaaacagcac cagcaccagt tccagaggcg atgccgccgc agtacgcgcc gcgagatcga
    17221 tctctttata ttgcgaatag ccgtcctgta cttggtacat ctttagggat attccttctg
    17281 agttacgcga atgccgccat gctggctttc atggtgtaca cctcgaccag cgtgttggtg
    17341 tactcctcga gataacggtc gtagttggcg ttataggtat cagtaagctg atcgccttcg
    17401 tcctgaattt tgctcatctg atcgtcaatg ttctgctggc gcagggtgat gatgccgttg
    17461 ctggagtccg tgtaggtgtt aataaggtcg tccatctgcg ccaccatgct gttgtcgcca
    17521 acgaaaatag aggtcagacc gtcaggattt ttcgccattt cgtcgttgaa ctggtcggag
    17581 tcaatctgta agtggccgtg agaatcgagg gtgatgccat agtcaacaat cgacacgccg
    17641 ttgtagctgg catgggcgat gtcatcgagc tggttcgcca gtgaactgag tccggcgtcg
    17701 cctgcaaata ccccggcgct ggtactgtca tcaccgtggg tggttagcga atcaaccgta
    17761 tcaatcaagg tgttataggc atcgacaaag gtctgtactt tctcctggct ggcgctggaa
    17821 tcttcgctga tgttaaaggt agtgagatcg ctgtcagaat cgctgacttc ggtgaaagtc
    17881 atggtgacac cgggaatcac atcatcaaac gtattgctgc tgttggtgat ctcaggcccc
    17941 gtggcgctgc caagatgaat aatcgcgtcc tgagcggagg aaacatccgt ggcaacgggc
    18001 gcgctggtgc tatcgttgct ggcatcatgc cccgttacgc tcaccgaaaa cgcgctctgc
    18061 gccccggtgc tatccgaggt gagcataatc gttgtggtgc cgtcggtttt taccagcgcc
    18121 gccgacacgc ccggattatc atcggagtcg ttaatcgcat tcaccagttc actggcgtcg
    18181 ataaaaccgt cgccgtcacc attctgatct gccgccgaca gatcgatatc catggtgctg
    18241 tcgcccatcg tcagttcaaa agtgccggtg gcggaaaagg cgtcgtcgcc catactgaac
    18301 gtggtttgct gcccctgtgc cagttgctca acgaaaaatg agtagcttcc cgcctgcgcc
    18361 tgggaattgg cggaaacgct cgccgagtca ttattgctgg tggcggcaaa cgtcaccggg
    18421 ccgtcggtat cgctgttcag cgcgtcaacg gcgctctgaa aatcgctcaa cgctgaactg
    18481 agcgaatcca ggccgctgct ttcagcatcc agctctgttt gcttctcctg caagtttgcc
    18541 gcctgagtgg cgacatccgc atacgcgatt tcttgcgcaa tggttcttgg gttgatcatt
    18601 gatgactccg gataacgatg tcaggttatt ggcaaaaggc gtgccaatat tttttatgtg
    18661 aataatcaat agattaaaaa acaggcggaa atggcgtttc cggtctggcg gaaaaaagcc
    18721 tgccgcagaa agcacaacac cgcccgcagg cggtgttgtt cgtagtccga ataaggcgtt
    18781 tacgccgcat ccggcaagtc cgccattact gcagcaggct ggaaaccatg ctggacatgc
    18841 tgttggactg tttcagcatg gtgatgccag tctgcatcag cacctgctgt ttggtcatat
    18901 tggacgcttc cgtcgcgtag tcggtatcca taatgttacc gatagcgact tcggtgttgt
    18961 cctgcatact ggtcaggttg ttggcggtgt cgttcagacg gttgatagac gcacccagct
    19021 tggactggat ctgcgaaacg tcatccatcg cggtagagat gctggtgatc atctggttag
    19081 ccgagccaga cgcggtcagc tcagtaccgc cagaaacacc tgcagtaccc gtggtgtctg
    19141 cctgatccgc gctaaaggag ttgctgatag cagaaagatc ggtcaccagc gtgttcagtt
    19201 ggctggagat gttcacggtc atggtgtcgg aagattccgc gcccacctgg aaagttaccg
    19261 cgctctggaa cagaccgtca gtaccggtgt tgctggtacc ggaaacaccg aacaggttgg
    19321 tgccgccgta agtggtgttt tgcagcatgt cagacatctg ctgacccagt tcgtcgtatt
    19381 cgtcctgcat cgcctgcaga tcgtcgtcgc tgtaggtgcc gttcgctgcc tgagttgaca
    19441 ggtctttcat acgacccagc acatcagaca tttcatcgaa catactatcg gcggtttgca
    19501 gcatcgcggt tgcgtcattg atgttgctca gcgccacgcc cataccgctg gactgtgctg
    19561 tcagacggtt agcaatttgt ttgcctgcgg cgtcatcggc agaagagtta atccggttac
    19621 ctgtcgccag gcgttccata gatgtagaca aagaagagct gcttttgctg attgcattaa
    19681 cggcggccat tgaggcgtta ttggtgttaa tagataacat gatactgctc cttgggatta
    19741 atcgttttcg tttcgatacc ctgtaaaaac gacacctaag gagcaaactt aaaaccgccg
    19801 ttaaaaaaat ttcactgcga tttatctgat gccactttta gctaaaaaaa gcagagttta
    19861 aattcagata aaaagattat tagcaatatc tgccacatgg ccaggtatca aaatatgtct
    19921 ttatcaaacg cttgatatag ttaagattac catcaattaa cgcgttttct tctcaatgta
    19981 ctgatgccaa aatttacatt gcgttgcaaa tagtacacat ttggctattg taccagtcct
    20041 gtcatttcgt tacatttcca tgccatatag tgattaagaa taatcctatt accataaaat
    20101 attagttatc acgcagaaaa atcatcttct cagggacgta taagactgtg ctttatgcaa
    20161 agcactatca ggaaacattt atcccttgct aaggtaaact caactatgag tattattatc
    20221 aacaactggc ggatggaccc gtcgcttaat gccttaatac attgcgaaac gggtgaaaca
    20281 cgtcgtctcg gcgaatacca ttttattttg ctggaaaaat tagcaaaaaa tgccgatgtg
    20341 gttttatcgc gatcttattt atgtgccgaa gtatggaaga accgcattgt cggcggaaac
    20401 agcctgccga cggcgatcca tgcgttgcgc gttgctatcg atgatgatgg caaacaacag
    20461 aacattatta aaacaattcc gaagaaaggt tacttatgca ataaagagta tgtgtctttg
    20521 ccggactcat cgcctgcaaa aaaactgatt attaccgacc agatacagga aacagtacca
    20581 gaagagatat cttctacaac accgcctgtg cctgttagaa aaaaacataa agtaataatg
    20641 gggctagcgt tagccgcggc tgttatattt attggctcga ccgtggggta ttcacacttg
    20701 aaaagtacac ctgatgcccc gcaactggta aaagagtcaa ttaacagtcc aagaataaaa
    20761 atatttcacc ttagttctgg aaaagaaaat aattccgtac cgttactgtc acaaaccctc
    20821 gcaccaggaa aagacaaact cgataattta ttgtcagcgc ataatatgac aatgacaacc
    20881 tattataaat atgttcgtaa ccgactggaa agtgatattg ttctgcgcaa ccagtgcaat
    20941 ggtagctggc aattaacttt taatgtggaa ggctggcaga acagcgatat taatagcgcg
    21001 atgtatcaga atttagagaa gttgttaaat actttgcaga aatgctgatg agtgccaata
    21061 tatgccggat gcgacgctaa acgcgtctta tccggctgat aattacccca ataaattcct
    21121 caccgtctgg ctggaaattc gcgcctgacc attcaccgcc tgcaataaaa cctgatagtt
    21181 atgattcgcg ctatccagca cgccgccgag gttttccgac gcctgcaccg cgttttccgt
    21241 ttgcgggaag cgcgacatgc cgtcgatcaa ctgccgcgcc ttttcctgct gtaccgccat
    21301 ctgcgcacgc tgctgattaa tgctttctaa cgtctgagaa attcccgcac cgccctgttg
    21361 caggctttga gctaaacggt ctgcctcaga cggctcggca aaagttttca gcggcataaa
    21421 cgccgactta tcgccatcgg tgcgcacgct taaggtacgt tcgatatggg gccactgttt
    21481 ttcggtggtg gagaaactga tgccgtccgc ttgctgatgt agctgcacgc ccacgcggcg
    21541 cagggcgttg gtcagccgcg tctgatactg cgcggcgctg tcatcctcag acagcatcac
    21601 cgccgaaagc tgagtctgcc gcccgtcaga gacgctgaac atgcgcgttc ccggcgtggg
    21661 attgtgtacc agattcgcca gatccggcga gtgaaaggtc acccgcgctt cgccctgtaa
    21721 caccggctgc aactggcggt ccaccgcacc gccggaaagc gcggtgcgtt tgtccagcat
    21781 ctgcatcagc gccgtattct gcgcctgccc gcctttacgc tggctgtgac gatagtcgag
    21841 caactgctgc tccagttgcc cgagataatg atccgcctgc tgcaacgtgg tgagctggtc
    21901 gttaagctgc acgctgtaac gctgcgggcg agtggtaatc agcggcgagg ccggatattc
    21961 actcgccgtc gccgggagtt tttgccgcac cgcctgagtg ggcgcaacgg ttgacgacga
    22021 taccgcagcg ctatgcgcgc cgccggtggc gagtgatgaa gatgtaagtc cgacctgcat
    22081 actgttcctg aaagagttag agaacgctga acaaattcaa ctcactcact ttcgagtaag
    22141 tcttgagggt aacttccatt gccacttcca gtccggtaaa ggtgatcgac gccgggccgt
    22201 aatcgagatc ctgtaatgaa ccaatcagct catcattaga ggtggagata tcggtctgcg
    22261 catcgctcag catcgacatg gtattttgcg tctcgccgag tgacgcaatc gaggcgttga
    22321 ggtcgtccga ggcggtgtcc accacatcaa cggcattctg aatatcgctc tgcacctgcg
    22381 gatctgccgg gtcgacgtcc gggttttgca actcctggga tagggaattc agggtgttga
    22441 gcacgtctaa attgctgcca aagaaatcac tcgccgccac gttggtgtcc acttccacgc
    22501 cgttcgacac cgtggtctgg cggtggtcgc tgttaccctg atagctgtag ctgtcggtga
    22561 cgccatcgcc gtcatcgtcc actgccacaa tcggcggctg atcgttgatg gtgccgccaa
    22621 acacgtagtg cccgtcttcg tcctgataat tgagcgccgc caccattgat tcggtgagcg
    22681 actcaatatc ctgcccgagg cttgcgagtg aatcggcggt attggtgcca ttcgccgctt
    22741 ccagcaggtc gtcgcgcacc gccagcaggc tgttgttgac gccatcaagg atcgactcct
    22801 gctggcttaa gcccgccgac gcggagtcga tattgctctg atactgctca atcgccgact
    22861 gctggcggtt taactgggtg attcgcgtgg cggcgatcgg gtcatccgac ggctgcaaaa
    22921 tctgcttgcc ggttgccatc tgctcgacaa catgcgccag atcgccagag agattattaa
    22981 aactttgcgt catcgagacg taggtttgtt gggtagttac tcgcattttc gcgctcccgc
    23041 ttagctgcac atttccagca ccgaatcgaa aatctcggcc ccggcggaaa tgactttcag
    23101 attggcttca taaatttgtt gataggtgat caggttcacc gcctcttcgt ccatgctgac
    23161 accgctgacg ctgctctgct ggttttgcgc tgcggaatag acattggacg cggcatcgac
    23221 ttccgtctgg ttttgctgac tgtaaatgcc gatattgctg atgattgacg agcacgcctg
    23281 cccgaccgtc acgctgccga ggttggctat ttccagcggt tcggtggaga tattgatcag
    23341 cgcctgcagg ttgtcgctgt tgccgctttc gtccggtgaa ctggaaaacg ccagctcatc
    23401 ggcggtgata ttcgggttaa cggtcagcgg gccatcggca ttgctggcgt cataaataaa
    23461 cagcggctcg ccggggttac cgttgagatc gtagccctgc gccagctggt tgttgaccgc
    23521 atcggcgaac tgcgacgcca tgctgttgat ggtgtcggtc agcggcgtca gcacatcgtt
    23581 ttgataatca aacagtgcgc ctaatgaacc gccggtgtcg gtggtcatcg tcgaggtggt
    23641 gccagcgaag gtcagcgaca tggtcgacgt gccatcggcg ttggtttcca gcgcgatggt
    23701 cgaactttgc tgcccactca ccagcggctg accgtttttc agggtaacgt tgtagttgcc
    23761 ctggtcgtcg atattgacct gcacatccat catcccgctc agttcttcca ccatctggtc
    23821 gcgggcgtcg tacagcgcgg aggcgttatc gccattggct tcagcctgag cgatttgctg
    23881 gttatagctg gcgatgccgc tggtgagcgt attgatttgc gacaccatcg cctgctgctg
    23941 gctgatgatt tccgtgcttt gcgagtcgat gtaatccagc gtgttatcaa tacgcaacga
    24001 cagcgccccg gcttcactga tcacctgctc gcgcagggcg gaatcatcgg ggctggtggt
    24061 tgcttcgtta agggcggcga agaagttatc gaagccgccg ctcaggctgc tgttatcgtc
    24121 gctcagtacc gcttccagtt ggctgagatc cccctgctgg gtgctgtaat agccgtaatc
    24181 gctggcggca taccacacct gattcacctg atactggttg gagacgcggc gaatgctgtc
    24241 gacctgtacg ccgttaccgg cgctgttcgg cgaaccgccg ctggcaccga tggtgctgat
    24301 ctccgccacc tgacgggtgt agcccgtggt catggcgttg gcggtgtttt gcgccgtgac
    24361 gttaagctcc acctgcgcgg ttgatgcacc gctgtagccg atgttaatca tgtccatgtt
    24421 ggatccttac cggtaaatcg tgccggttaa aagcggcgta aagttgggga ttcttaagtt
    24481 ccgtaaaccg ggtgccgaac gccggatgcg gcgtggacgc cttatccggc ctacatacgg
    24541 gcgactcgta ggcccgatac ggcatgcgga ctgcaggcag agaaaactgg ccgattttct
    24601 ctgcactgga gggataatac aaggcctgat aagacgcgac agcgtcgcat cgtgcaccga
    24661 acgccgaagc tcatttatgc ttcggcgaca actgcttcac aatcatcgcc gcaatgccaa
    24721 tcccgtgctg ctgcgccagg gtgttacaca actcatcgtc ataaaacccc tgcatcatgc
    24781 gcaccgaatc gctgttaaac gggttatctt ttgaatcaaa caactcgttg gttttccgca
    24841 tctctttcag catgttgcgt aaaaaaatcg cctcaaactg ttcggcggcc tgcttgatat
    24901 cattggcctg aaccttcggc cccattaacg cgtcgctgcc gtcaatcccg ccgctggcgt
    24961 tcactttcat cagatcacct ccaggtcggc atccagcgcg cccgcctcat gcagcgcctg
    25021 caaaatcgac ataatgtcgt ccggcgtcgc gcctaaactg ttcagcgcat tgacgagcgt
    25081 tttcaggctg ctcgactccg gcaaactcac cacgcccgga cgggcatgat tcaccgcgat
    25141 attgctctgc ggcgtaaccg ccgtgcgccc acccgcaaag gcattcggct ggctgacgtt
    25201 gctggtttcg ttaatcgaaa cggtcaggct gccgtgcgac accgccgccg cgtgtagcgc
    25261 aacgccatcg cccattacca ccgtgccggt acgcgagtta aacaccacgc gagcgcggat
    25321 tttctccgcc tgcacctgca catcgtccag ttgcgacata aatgccaccc gcgcgccggg
    25381 gctggtgggc gcgcgcacgg taacgttggt ggaactctgc gcggtggcaa taccgccaaa
    25441 cgaggcgttg atcgccgccg caatgttgtt ggcgtcttta aacgacgggc gtttcaggtt
    25501 gagggtgatg gtatcgccca tctgaaaatc gctggggatc tcgcgctcca ccgttgcgcc
    25561 gttaggaatt aacccggcgg ttggcgtatt aaccgttacg ctggaaccgc tggccccggt
    25621 ggcgttcatc ccgcccacca ccacgctgcc ctgtgccagg gcgtaaactt ccccatcagc
    25681 gccgtgcagt tgggtcagca gcaaagtgcc gccgcgaata cttttggcat cgccaatcga
    25741 cgaaacggtg acatcaatgg tctgcccgcg cgagtacatc ggcggcagcg tggcgctcac
    25801 cgccaccgcg gcgacgtttt tcaccttcgg gtcgatttta ttcggcagtt gcacgccaaa
    25861 ctggcgcagc atattggtga tggtctggtt ggtgaatttg acctggtttt tatcaccggt
    25921 gccgtcgagg ccgaccacca ggctatagcc caccagttgg ttttcgcgca cgccctgtac
    25981 gttgaccagc gactccagcg attgcgccag cgccacacca ggcagcgcca ggctcacggc
    26041 gataactgcg gcctttatcc agttttgcat tgcactctcc ggcattaaat cgggaacaga
    26101 ggatggttaa acagccgcgt cagccagcct gcggcgttgg cgtcgcttaa cgcgccgcgt
    26161 ccggcatagg agatgcaggc atcggcgatc cgctgggagg aaaccgagtt gtcgttctgg
    26221 atgtcgtcgg cgcgcaccag gccgcttaag cggatgtact catcgccctg gttgagggtg
    26281 agccatttct cgccgcgaat ttccaggatg ccgttgggct gcaccgcatg gacggaaacg
    26341 gtgatctccc cgcgcaggct gttctgctgc tgggaagtgg cgctgccgtc aaagtcgcgg
    26401 ttggcgtcga tagagccgga aagcttatct ttagtgtgac taaaaatggt cggtgcgccg
    26461 atatcgacgg tgttgctttt gccgaagttg gttttcgcct gtttgctcga ctgggtggat
    26521 tcttcgagga tcaccgtcag gatatcgccc acgcgataag cccgccgatc ggcggtcagc
    26581 gaccagttgt agccagtttc aaatacaccg ccagcacgcc cgtctgccgg aggcggtgct
    26641 tcggttttcg gtggcgcaaa gtatgcgtcg tcttttttga ccagaatggc ctgcgattca
    26701 cacccgctaa gcaggggaag cagcgccacc agccagagat aatttttcac gttctgcctg
    26761 tttaaataca gtgagttatg cgcttttgta ggtcggataa gacgcgtcag cgtcgcatcc
    26821 gacatttcgg ccttacagcg tctgactgat gtactgcaac atatcgtccg cagccgacac
    26881 cattttggcg ttcatctcgt aagcccgttg cactgtaatc atcgccacca tttcgttgac
    26941 gatatctacg ttcgagcctt cgagggcatt gtcctgcaaa gtgccaagcc catcttcgcc
    27001 cggcacacct tcggtcggct gaccgctggc ggcggtttcc agatacaggt tgtcaccctc
    27061 agcggaaagc ccagccgggt tagcgaagtt aaccagcgtc agttgcccta actcggtcac
    27121 gtcgctgtcg ccaggaagaa tcgccgtcac cgtgccgtcc tcaccaaacg caacattcgt
    27181 cgcccccgcc ggaacgtcga tttccggctg caatggcaag ccctgtgagt tggtcagtac
    27241 gccatcggcg ttcacctgca aattgccgtc gcgggtatag gcgatatcgc cgttggcctt
    27301 ttgcacctgc aaaaatccct gccccataat ggcgacattc atcgcgttgt cggtggtttc
    27361 gacgttgcct tcggtgaagg ttttctgggt gccgacaatg cgcacgccgc taccgaattg
    27421 cagcccggtc ggcatgatgt tgttctggtc gagcatcgcg cccggcgctt cctgggtctg
    27481 gtagaacaga tcctgaaaca tcacccggtc acgcttgaag ccggtggtgt taacgttggc
    27541 gatgttgttg gcaatcgcgc tcatttcagc atcctgcgcc gagaggccgg ttttgctgat
    27601 ccataatgct gcgttcatgg tggtaaattc cgtgttattt ttgggccgcg gttgccggat
    27661 gcggcgtgaa cgccttatcc ggcctacgat gtgcggttac gagccgcgca ataagcggtt
    27721 gcccgcgtcg ctgatatctt cggcggtttt catcatcttg atctgtgctt cgaactggcg
    27781 gttcatggcg atggacgaca tcatttcgct taccgccgag acgttgctgc cctcgagaaa
    27841 accgccgctg actttgatgt tttcatcgcg ctgggccgga acgccgtcgg cagtcaccag
    27901 catcccttca gcatttttct ccaggttagc caccgggata tccaccagct tcaggcgatc
    27961 gatatccatc gtcgcggtca catcgccgtc atcgggcgtc accgacagcg tgccgtcgct
    28021 gccaaacgaa gcaatggcgt tcggcggcag aatgatcggc ccgttatccc ccagcaccag
    28081 attgccgtcg atggtcagtt gcccctggtc gtcctgctga atgttgccgt tgcgggtata
    28141 gacttcgtta ccgtttttat cctgcaccgc aatgtaaccc gcgccctgaa tcgccacgtc
    28201 cagcgggcgt tcggttttct ccgcaacacc cgtgctgtcg ttcacgccgc tggccccttc
    28261 ctgcgccata tagcgcgtgt cgaagccgcc gcctttcacc ttgtcgttgg ttgccattgc
    28321 catatcggcg cgaaaaccat tggtgttgac gttcgccagg ttgttggcgc taatttgctg
    28381 ctcatacagg gtctgagaag caccgctcag ggcggtataa atcaggcggt ccattacatt
    28441 gcctggaaca gcgcgtcatc gagctgcgtg ctggtggcga taactttggt gttggcctga
    28501 tagttacgct gggcggtcat caggttgacc agttcgctgg tgatatcgac gttagagctt
    28561 tcgagcacgc ccgacgacag cgtaccgcag gtgccggagc cgggaacgcc aatcagcggg
    28621 gtgccggatt cgcccgtttg tacccatgcg gtgccgctca ctgcctccag gccgttttca
    28681 ttcgggaaag tcgccagcac cacctgcccc tgcaacatgc gctcgccgtt gctgtaggtg
    28741 gcgtaaactt tgccatcgtc atcaacctga acaccgtttt gcgtggcgga ggcgtagccg
    28801 ttggcggcgt tggtggtgac cgaaaattca gagccgtatt gcgtacaggt ggagtaatcg
    28861 acggttaagt cgacgggcgc ggcggcgtcg gtttgaaatt caatggtctg cggcgtggtt
    28921 ggagaggtca gcttcccggt gttcggatcg aaggttaacg tcgtcgccgg aacgccggtc
    28981 tgctgctgac cgtcgaaggt gtactgcact tcccaggtgt tgtcgctggt tttggtgaag
    29041 tactggcata ccgaatgttc gttgcctaat gagtcataca ccgtggtggt gtagctgtcg
    29101 gtgtacgaac tgctgttagt cgcgtcgaac ggaacggtgg tgcgatcgat agcttcatcg
    29161 ctggcgttga agttagcggt aaaggtcagg ctgctgctgg cctgcgccgg gatattgccg
    29221 gttttgatct ggatatcggt gaccgtaccg gtttgcagag tgccagtgtc atcgaccgga
    29281 tagccctgca aataagcgcc cgaggcgttg acgatatagc cgtttttgtc ggtttcaaac
    29341 gatccggcgc gggtatagga gatattgcct gcgctgtcac acatgacaaa aaatccgtcg
    29401 tcgttaatcg ctaaatcgag cgcattgccg gtggagacca gcgaaccgcc acgcgaaata
    29461 ctttgcgcgg tgcccgccac gctgacgccc atcgcctggc ttccggcata catggcggaa
    29521 aactgggtgg tcatcgactt atagcccacc gttccggcgt tagcgatgtt gttactgatc
    29581 ccgtccagct gttcgttaac ggcattcagc cccgttgcgg caatttcata actcattgtg
    29641 tcttcctgtt aattgattct tacgccacat cactgtcggc atcgggtacg ccaaactggc
    29701 taatcatggt gaacggcact tcgcccacgc ctgcaacgtt aagcaccggg gaactgccgt
    29761 cgagcgggat gcgcacatct tccacttcgc cggagacttc aatcggcacc tcttcttccc
    29821 cggtattggt caccaccgag acgtcgtaat cgcccggcgg gatgccgtaa tccgcgggat
    29881 tcaccgagaa atcaacctgg cctggtccga cggacgaact gccttctggg atcagcgaga
    29941 cggtgtagtc atcgcctgcg gcatcggtaa agtggagatc ggcggtggta caggcgtcgt
    30001 ccagcgtagc gcgaccgtta atggtctggt cgctcacctg caaggcggtg gtctgcacca
    30061 taatgtcgtc gcccaccagg ttaccgagcg ccatcacctg aatgttgctc atcagcaccg
    30121 cgttggtgtt ggcctgcacg ctcatctctt ccatcatcgc cacctgcgcc atcgacgaca
    30181 gctggtcgat gtattcggta gcgtcggtcg ggtcggtcgg gtcctggttt tggatctcgg
    30241 cgacaaacag cgtcaggaag gtatcgacag gcgagctatc gctgctgctt tgcgtgtcgc
    30301 tggccgccac attattgctt tccgccgtgg cggtcggctg cggcgattgc gcattcagcg
    30361 ccagggtatt catcaggctt ctcccagttt cagcaaactt tgctgcatac ttttcacgtt
    30421 gttgagcaca tcgacgttgg tttcgaaatc gcgcgaggcc gacatcatgt ccgccatctg
    30481 ctccaccacg ttgacgtcgg gataccagac gtcaccgttg gcgtcggcga gcggagaatg
    30541 cggttcatag cgtttcaccg ccccgccggt ttgcagcacg tcctgtacct gcacgctggc
    30601 accgtcaatg gcatgacgat gtgtacccgc cagcaggctg tggtgataca ccgcggcaaa
    30661 caccgggctg cgcgctttat acgcctgcgc ctcgctgctg gcgggcgcat tggcattcgc
    30721 caggttactg gcaacggtat tcagacgtag cgtttgcgcc gtcatcgccg agccggaaat
    30781 ctgatagata tcagtaaatg acatgcttat ttcccttcaa tggcctctct gatgccgctg
    30841 atttgcagat tcagaaaggt caaactgctt tgataatcca tactattttg tgaaaaccgc
    30901 gcctgctcgc tgttcagttc cacggtgttg ccatcttccg acggctgcat cggcacgcgg
    30961 tactggacat ccaccgccgc gttatttgcc cgttgcatct ccctggcaaa atcaatatct
    31021 ttagccttga aatttggcgt atcgacgttc gccagattcg cagtcagtag ctcggtcctc
    31081 tcaagacgta acttcactgc ctgcggatgt actcccaatg cctgctgaaa actgatcccc
    31141 atttcctgca tcactcctgc ttcaatccgc atacgctaag caatcgccat gccatttttt
    31201 aagtcactga tgagaaaggg tttatgagtt ttcaattttg caggcggaag ttttacttcc
    31261 tcctggcgct ggccttaccg ggctacgccg ccgtccatcc cgtgcaacac agcgcgcggg
    31321 aacaagtcaa cgcccaggtg ctgaatgccg cgagccaaaa aattgaatcg ctggcgcagc
    31381 agcggcagtg gcatgactat cgctacacct ttaagatcta cattccctcg cagattgcga
    31441 ccgccgcgcc ctgtactaaa acacccggcg tcacgctgac ttcgcctgcg gaaatcgccc
    31501 tcaaccggat gaacttcacc gttagttgcc cgcaaagctg gcagatgaac gtggcggtgc
    31561 gcccggatgt actggtgccg gtggtaatga cgaagtcact ggttgcccgc gatatgccgc
    31621 tcaccgcgaa tgatgtggag cttaagccct acaacgtcag cgcccagcgc cgcgatgtgt
    31681 tgatggagct tgatgacgcg attggtttaa gcagtaagca cgcattgcaa ccaggcagac
    31741 ccatcaccaa agaagaactg gtgtctccgg tgctggtcgg gcgcgatcag ccggtgatga
    31801 ttgtttacca gagcgcgggg atcaccgcct cgatgcccgg cgtggcgctg aaaaatggtc
    31861 gtaagggcga gatggtgaaa gtgcgcaatg ccagcagcca gcgggtcatc agcgcgatgg
    31921 tggcggaaag cggcgtggtg acaacggtaa gcgcggagta atttgcgcag gcgattaaag
    31981 ttttgccgcc tgttaaccgc tttaccgcag taacccaccc aaataccgag acgaggttgc
    32041 gatgaaaatc accccgacta tgcctggcaa tcgcccgaca gccacaacgg caggctccag
    32101 ccagccgcgc aaaaccacgg tgaccaccac cacgacgctg agcgccgacg acattactca
    32161 ggcgggattg cagtctgccc agcaaacgct gaacgaccag caagagagcg atatcgacag
    32221 caacaaagtg gcgcaagtgc aggcgatgct ggcgtccggc agcccgcaag tggacactga
    32281 acagttagcc agcgacatgc tgagtttttt ccagaactaa gaggccgatg aagtgagtat
    32341 ccggttacaa caggtgaagg cgttgttgca gggcattcgt gacgatgacg cgctgtatga
    32401 ttccctgcgc gaacttctcc agcgtcagcg gatctgcatg attcgccgcg ccagcgaaga
    32461 gttgctggcg gtaaacgatg agataaccca tcactatgag cagttgcatg gacatagcca
    32521 tcagcggcat agcctgctga aaatgctcgg tgttagcgta aatcgtgacg gtctggcgca
    32581 ggttttcacc tggcttccgg ctgttcaaaa agcggctgca caacagttgt ggcaacgcct
    32641 tgagcaaaaa gcggaacgct gcaaagccta caacgacaaa aacggcgagc tgttgattcg
    32701 ccagtatgaa tttattcagt cgtttttagg cagcgaagca gattttctct atcaggaata
    32761 acgcgaatac cttccccata gcggaaatgg attttccgcc tgatttccaa tcccttgttt
    32821 ttaattacaa aaatccccgg cacgcttatt gcatatcctg cacagcatta aagactggaa
    32881 gccacctatg agttttattg aatgttacga accggaatac gtgcgcaaat ttttagcgcg
    32941 tcaccccggt tcgcctttat acgtccgcaa ggtgtggaaa aacgagattc gcctgagcct
    33001 gtcattgacc gatattgcca gctgcgaagc ggtgctcaac gatgtcaggg cctttgattt
    33061 acagctcacc taccgccctg tggaagataa ttccgcagag ctttccgcgc gcgatgcgat
    33121 agtaaaccag gtgattttga gtaccctcac gctccgtgat ttaaccccgg agctttccct
    33181 gtatgctgtc gggattttgc tctcctgcgc cagcaaaatg ccgggcagag acggagacat
    33241 cctggcccgt ttgacgacgc tgccacaggc gctggccgat catgcgaaaa aaggtacgct
    33301 acaggcacag tacgcccagc tcccgcccgt gccacagctg gcgcgccagt tgatgacgct
    33361 gctcggcggt tgtgcgtttg actggtcgat tttgccggaa agcccgcgca aagcctcgct
    33421 gccgttgcag gttactttac tgacgctgca tgacgccaac agcgaagccc tgttacaaca
    33481 gcagcttcag acgcagtggc aaaccacctg gcaacagcat tttgccaccg cgccctggat
    33541 gatgcgtaac tggctgattt accgcgttta ccacgatatg attgggcaga ctgacggggc
    33601 ggattatttc ccgctggtgt gcgattttta tctcctccgc acgttaatca gtttatggac
    33661 gctggatggt tctccgctgc ggcaggagga tattttcgcc ctctttgcca tgtttgaacg
    33721 ctggcgagcc agtgaaaatg cgctgttggt tcgccagcaa attcagtcgt tatgtgccgc
    33781 cgacccgctg ctttcagcgt tttcgctgct aacgtgagtc gctctgcgcg cgctttgccc
    33841 ctcaccctaa ccctctcccc aaaggggcga ggggaccgat cgagcacggt gttaaaatcg
    33901 gcgctgattt tctccctctc cctgtgggag agggtcgggg tgagggcatc agcgcgcacg
    33961 ttcatcacat tcacacatta actcacccaa acaccatgcc aacaccaacc atcatcaaaa
    34021 cactcgccac ccaccgcgaa aaaccgtttg tcagcgtcgt cacgccaacc tggaaccgcg
    34081 gcgcattcct gccgtacctg ctctatatgt accgctatca ggattacccg gcagaccgcc
    34141 gcgaactcat catcctcgac gattccccac aaagccatca acacatcatc gaccgcctga
    34201 ccaatggcac gccggaagcc tttaacatcc gctacatcca tcacccggaa aaactgccgc
    34261 tgggcaaaaa acgcaacatg ctcaacgaac tggcgcgcgg cgaatacatc ctctgcatgg
    34321 atgatgacga ctactatccg gcagataaaa tctcttacac catcgctatg atgcaaaaac
    34381 accgggcgct catctccggt tccgaccaga tccccatctg gtacagccat atcaaccgta
    34441 tctttcaaag ccgcagcttt ggcccacata atatcctcaa cggcaccttt tgctatcacc
    34501 gtaactacct gaaaaagcac cgttacgacg atgattgcaa tttaggcgaa gagaaaagtt
    34561 tcaccgacaa tttcagcgtt aacccgctgc aactgcccgg cgagcgcacg attttgtgta
    34621 tctcgcatag ccacaacacc tttgataaag actttattat cggggccagc acgccggtaa
    34681 acacggcgct tacggatatc gtccgcgatc cgctgctgcg caatgcttat ctgagtctgc
    34741 ataacgccac tcatcaccag gcgatcaacc atcaggcgat cgatcaggtg gtcgtcgtga
    34801 atctggataa acgcccggat cgcttacaac agatccgtga agaactggca ttgctgcata
    34861 ttccacccga gaagatcacc cgccttgccg ccagtgaaga tgaaaacggt caacggggcc
    34921 gccgtcagtc gcacttgcag gcgctgcgtc tggcgcaaca gcacggctgg caaaactatc
    34981 tgttactgga agatgacgcg gttattctca agcaggagaa acatattcag gtactgaaca
    35041 cgctgctggc gagcctggcg aaaattccct ggcaggtgat gatcctcggc ggcgaaattt
    35101 cgcaaggaac gatgctaaaa agtttgcctg ggctggtgca cgcgcgggac tgccgcaaag
    35161 tgtgcgctta tctggtgaac agccgctact acccgcaact ggcgcagcag atgagcaacg
    35221 atgagcactc gctggaagac tgctggcaac cgctgctacg cgccgacaaa tggctggcct
    35281 gttacccgag cctttgttat cagcgaccgg gctttagcga tatccagaaa aaaacgactg
    35341 ataatattgc tcgctatttc aataagttac ccgtaaccac caagccatcc gcgttaccca
    35401 tcgccgacac catcggcttt tttatggaaa cctcattcca ctacgcgctg taccgcccaa
    35461 tcatcaccgc cctacaggcg caagggctga gctgcacgct ggtgatcaac gaccgcgtgt
    35521 ttaaaccgtt tctggatgag atgctggaaa cactgaaaaa catcgacgat ccgcagctta
    35581 aaggtatgcg tctgtcagaa atgcagacac atggtcagcg ggtgaaatgc ctggtcagcc
    35641 cctaccacac gcctgcgctc aatggcctgg cggcggtgaa catccgcgct atgtacggct
    35701 tagccaaaga gacgtggaac cacgccgact ggaatcgttt ttaccaaagc attttgtgtt
    35761 atagccacta cagtcagcag gcgcttgcgc atttcggcag cgcaaaagtg gtcggcaacc
    35821 cgcgcttcga cgcctggcat aacggcacgt tcgatcgcgc gttgccggaa aacatccaat
    35881 cagattaccg taaacctacg gtgctctatg ccccaacgtt tggcgcatta agctccctgc
    35941 cccactgggc tgaaaaattg ggccgcttga gcggtgatgt gaacctgatt tgcaaactgc
    36001 accatggcac ctgctcacgc ccggaagaag cagcatcgct ggccctggtg cgcaggcatt
    36061 taaaacagcg caccgactcc gcccgccaca cgctggcgct gctggcaaaa gccgattacg
    36121 tgctgaccga taacagcggg tttattttcg atgctatcca cgttgataag cgggtgatcc
    36181 tgctcgactt tccgggcatg aatgatttgc tcgacggtga gaaaagttat tccacggcag
    36241 agagcgccga ccagcgaatc cgcgaaatat tgccggttgc tcatgatgtt gcagaactgc
    36301 gttatttgtt gtcagaggcg tttgactggg ggtcagtgca ggcgcggctt acggagattc
    36361 gccatcacta ttgcgatgcg tttatggatg gcaaggcggg agaacgggcg gcgattgtga
    36421 ttgtggaggc gctggggtaa accgcgcaac tgcctgatgc gcttctctta tcaggcctac
    36481 ggatcgatca cgtcctgtag gccggataag gcgtttacgc cgcatccggc aagaaaatac
    36541 atctctgttt tagtacttta taaaagccct ctaatcacct caataatccc cgtcgtcgac
    36601 accgacggcg tgcgggggaa atagaccacc tcacaaatat acgcaaagct gtcaaacttc
    36661 cctgcccagt catcccccat cactaaaata tccgcactgt acccgcgcaa atactccgct
    36721 ttctgctcta gcgattcttc cagaaatacg ccatccacgc acgccagccc ggcgatgatc
    36781 gccatgcggt catcctgatg gtaaaccggt gccctgccct ttttggcgat gttcagggcg
    36841 tcggacgata cgccgaccag cagccgctcg cccaatgcgc gggcgcgttg taaaaggcgc
    36901 agatgaccga catgaaacac atcgaaggtg ccgaaggtga tgaccgtttt catggctact
    36961 ccgctaattt gtgccgcagc cagcactgca cggcgatgtc gtccaccgct ttttgcgctt
    37021 tcgccgtggc gctttcttgt tgctcgcggc gtttgtcgtc cagcgtcagg gcgactactt
    37081 tctcttcgca cgccgccgcc gtgaggtttt tctgcattcg cgtggctttg atattggcca
    37141 gcgttttttc ctgctcctgc caggcgatta tcttgcgcag ggtgcctttg tagccgctga
    37201 cgtttttcag cgattccacc gacaccgccg cacccgcaga tgttttctcg acaaggtagc
    37261 ctaacgcctt gatattgttg tcataacccg cgcaaatctg tttttgcttt gccagctcga
    37321 cggttttgtc tttgaccgat ttatcccgca ggcgctgcaa ctgcgtcagg gtgtcgatga
    37381 tttgtcgcat agttactgtt ctgccttttt gacaccagga aagagaattt gcaggcggct
    37441 ttgcaccagt tcgagggagg cgggttcgcg catctcctgg cgcaaaaatc gctcgatggc
    37501 cgggaacatt tttaccgctt tgtcgacgct ggcgtcggca cctgccacat aaccgccgag
    37561 cgggatcaga ggtttgatct ccatataggc ggcatagttt tgctttaacg cgcgggcgga
    37621 ttgctgatgt tccaggctgg tgacctggtt catgcagcgg ctgatcgact ggccgatatc
    37681 aatggccgga tagtgccccg cttccgccag tttgcgggtt aatacgatat gcccgtccag
    37741 caccgcgcgc gcgcagtcga caatcggatc ttgctggtcg tcaccttccg ccagcacggt
    37801 gtagatggcg gtcatcgatc ccgcgctttc gctgttgcct gcgctttcga ccagtttggg
    37861 gatcatgccg aatgccgatg gcggataacc tttggtggcg ggcggttcgc cgagcgagag
    37921 ggcgatttcc cgctgcgcca tcgcgtagcg ggtcagggaa tccaccagca gtaagacgtg
    37981 atgcccgcgg tcgcgaaacc aggcggcgat ggagtggcac agttcggtgg ctttcagacg
    38041 catcagcggc gattcatcgg caggcgcgac gacgacgata gactttgcca gcccgtccgc
    38101 gcccagcgag tggtcgataa actctttcac ttcgcgcccg cgttccccaa tcagcccaac
    38161 caccacgata tcggctttgg tctggcgggt gatcatcccc agcagcacgc ttttcccgac
    38221 gcgctggcct ttgccgatgg tcagcaggcc gttgatcgct ttcacgccca cgtccagcgg
    38281 ttcgtctacc gaacgacgag ttaagggatt aaccgagggc ggcaacggcg gcagaaggtc
    38341 gttgccgttc aggcggcctt tgccatccag cggttcgccg aggccattca ccacgcggcc
    38401 taagcagctt tcgcctatca ggatgtcgtg ggctttttct tccgggaaga cgcgtgcgcc
    38461 tgccatcagg ccgaccggct gtttaaacgg cattaaatag gtgatgtcgc gattaaagcc
    38521 cacggcctgc gcgtcgatca gcgtgtgatt ggcactttcg atgcggcaca gctgcccggt
    38581 cattagcggg cagccgacac tctccagcag gatgccgtta aggcgcacca gccgcccggc
    38641 gacgcgggcc agcgggatcg actccagcga gcgcagggcg ttatcgaacg cagaaaaatc
    38701 actcaccctg cgactccggc gtgagtgttt ctttcagcgc ggtcatgcat tgttcaaggc
    38761 gatgctcgca gccgatatcc agttcggatt tgtcggtgat cacccggcat tcgcccggtg
    38821 gcaaatccgg cgaaggcgtc agcccccatt cgctgacctt ttccggcacc gcgtcgcgca
    38881 ggcggttaaa ctcttccgtg ctcagcatta cttgcagggt ttccggcatc gccgggaagg
    38941 ccgccagcgc ctcttccacc agcgccagca actgcgtggg ttgcagcgcc agttcacaac
    39001 ggatcacctg gcgggtgact ttttccacca gttgcagcaa atcttcccgc tgtttacgtt
    39061 cgatatgagc gaggaagtcg ttcactttac cggtgatggc ttccagcggc tgcgccgcct
    39121 caacaaactg tttgcgccct tcctgctgcc cggcgaggct gccttcggtg taaccctggc
    39181 gtcgcccctc ggcaaaacct tgctgatgac cttcgctaaa gccctgctcc tggccttcgg
    39241 tcattccttg agcaaaccct ttttgcagcc cttcctgaaa tccgtccatc aactggcgct
    39301 ggtaatcggc ggcgctaata ctggtagtga gcggttctcc ggtcaccgca cgctggcgcg
    39361 gcgggaaacg gtgcaggcga tagcgcccgc gcagtgtttc aatggtcatg gttactccgc
    39421 cgtctgttcc gcaaagagct gtagctgaat ttcgcccgcc tcttccagtt cacgggcgat
    39481 gcccatgatt tcgcggcgga tctgttcgat acggctgacc ggcaccggcc ccagacgcgc
    39541 ggtgatggct tccagttgct gcgcctgccg ttttggcatt accgcgtaaa tcgaacggcg
    39601 cagcagggct tcggtgcctt tcagcgccac cgcccagtct tccatcggca cttcgtcaag
    39661 cagacgtcga cgcacctctt cgttctggcg gctgaggata aagaagtcgt acatctcatc
    39721 ctgaagctgc tccagcacct cttcgtcgcg ctcgcgcatc tggtcgagga tcacctgctg
    39781 gttgccctgg aagcggttga cgatatccgc cgcctgtttg atgcctttca cttttgagcc
    39841 gtgttccgaa agcaccgaca ggccgcgttc gatcagcctg tccagttcat ccaccacgtc
    39901 gcggttgacg tcgttgagtt tcgccacccg gtagagaatt tcgttttgca ccgattcgtt
    39961 gagatacgag agcaccgctg ccgagatttc cggcgtcaga aacgcgagga acaccgcctg
    40021 taattgcaga tgttcttcgg agatcagcat cgccagctgg cgtggttcca cccattgcag
    40081 gcgcgccatc cgcgagcgga tctcatcgcc gtagatgccg ttgatcacac tgctggcgat
    40141 ctcggtgccg agggctttgt tgagaatacc ctgcaacatg gatcgcgatg cgccgttgat
    40201 cccgctctgt tcgcggaact catcaaagaa gttattgatc actttgcgcg ccatgctggt
    40261 tttcacgccg gaaagccgtg ccatgttttc gctcaggcgc accacttctt cgcggctgag
    40321 tttttgcatc accgtcgccg ccgcctcttc gcccagacac agtagcagga tcgccgcctg
    40381 ttccaggtag ctgttgctct tatttgttgt caattcgctc attgctggtg atccattgtc
    40441 tgagaacttc ggctacgcga tcggtgtcgc tcatggccag tttttgcagg aactcgagtt
    40501 ttacttccag gccggaactc tgagacggca ggctgtcatc gccgggcagc gacggcagat
    40561 cgacgttttt gcgctcctct tccggcatgg caatccacgg ttcgctgacg gcttccgatt
    40621 ctggtgtgat aatcggtgca cgtaccgccg tcagccgttt cattaccggg cgcaccacga
    40681 agaacagcaa caacagcgcc agcaggccgc agccaatcag gcgcacccag gccagcacgc
    40741 tgtcatcttt ccacagcgga ataattggtt cgaccggcac cgcctgcggc acaaagttaa
    40801 gcagtgaaag cgtcaggtta tccccacgtt gcgcgtcaat accggcggca ttgttcagca
    40861 acgcggtaag ctgcgcggtc tgctccggct tccagttttt cagcgccgga gcgttttgat
    40921 tgagcaccac cgccacattg aggcgtttca ccgcaaagcc gggatgctga atatgctgga
    40981 cgctacggtc gtaagagtaa tcgcgcttgc tttcgctgtg tttggataac gcttcaggcg
    41041 agcgattttc ttccgtaccg ttagtcattt gattcgccgc aaccggcgga cggttgctga
    41101 gagagccggg cacacccatc gccacctgat tggtgtcgct gtccagcacg ctctcttcgc
    41161 ggtttatttt cggcgcgtcg ccgtagtgtt cctgagtttc gtcgatggtg ctgaggtcga
    41221 gatccggcat cacgctgaca cggtaattcc cgctgccgac cagcgaatcc agcacgttcg
    41281 ccacgctggc gtgggttttg tcctgaatgt ctttgaggat ctgatcgcgt ttacgggtag
    41341 cggcagagac cgcctcgccc gcgccgatgc cgtcggtcag cagatttccc gcctgatcaa
    41401 ccacgctaac tttgctggcg tgcagccccg gaatgctgcc ggaaaccaga tgcacaatgg
    41461 cgttcacctg gtccatattc agcttcgcgc cgtagtgcag acgcaccacc accgaggcgc
    41521 tgttttgcgg ctcatcgctg accacaaacg agctttcttc gttgagcgcc agatgcaccc
    41581 gcgcgctctc caccgcgtcc agcgacataa tgctctgcgc cagctcccct tcgaggctgc
    41641 gtttatagcg cacgttctgc acgaactggc tgctgccaag cacttcgtct ttgtccatca
    41701 gctcgtagcc gctgggcaaa atcgcctgca cgcctttggc ggcaagggtc atgcgcgttt
    41761 tcgacagcgc ctcttccggc accagtatct gcccgctttg cgggtcaatg cggtaggtga
    41821 gcttttcgcc gtccagcacg gtgacaatct gcgacacggg cagattttcc tggcgtccgt
    41881 agagcgagac gtagccgcga ttgccggtcc acagcacgct aacgataatc gccgtcgccg
    41941 ccacggcaag ccccacaccc gccatcagcg cccagcgttt gttatctgcg agacgcagtc
    42001 gaaacgccgg aaacgccagg gttaacttct ttatttgtgc attcataggg ttagatcgac
    42061 atactcatca gatcgttaaa tccggtggcg attttgttgc gcacctgcac cagcgcggag
    42121 aaagagagcg acgcctgctg gctggcgatc atcgcacctg ccagatcatc gcttttgccc
    42181 atgtcgatag ccgtttgctg ctgctccgcc acctgctgga actggtcaac gtgaccgagc
    42241 gcgccctgca taatgcggtg aaacgaaacg tccgggtcgg tggaactgaa ggtcatggcg
    42301 ggcagcaccg gcgcctgggc gttggcctgc atccgctgca cgtcctgcat gatctgcgcc
    42361 tgcatggtgc tgttgattgt ggtaatgctc atttttaact tccgggtcag gagaaaactt
    42421 cgataccgtg cttgcgcatg gaggccagtc gatagcgcag tgcgcgcggg gtaataccta
    42481 acaggtcggc gattttgctg cggttgccct gatatttacg catcaggtca gcgatatatt
    42541 gatactgggc gcttcgtccg tgctggccta aatcgctggt ttcggcaatg tgtacggcgg
    42601 gctgagcagg ccattgcagt tcgctgtggt cggcaatgtc attttccggc aggcctaagg
    42661 cgtccgggta aatcacgccg tcgcgattta aaatcattcc ccgctgaatg gcgttctcca
    42721 gctggcgcac attgccgggc cagcggtaat tcagcatcgc acggcgggta gattctgaaa
    42781 gggtgatatt tttcaccagc accgtggagt atttttttat aaatgactct gccagcggaa
    42841 taatatcgtt cagacgttca cgcagcggcg gcatggtaat aggaataacc gccagacgat
    42901 aatagagatc ttcacgaaaa cgcccggcgg cgacttcctg ctcaaggttt ttattggtgc
    42961 aggcaatcag gcgaaaattg agtttaattt gtcggttgct gcctaatcgc tcaacctgtt
    43021 gttcctgcaa tacgcgtaat attttggcct gtaacgccag cggcatatcg ccaatttcat
    43081 cgagtaataa ggtgccgtta ttcgccagtt ccatttttcc tggcacactg gcaattgcgc
    43141 cggtaaatgc gcctttgtcg tagccaaata aggttgcttc cagcatgttt tccgggatcg
    43201 cagcgcagtt cacgccgata tagggcgcgt tttcattttc accaaaggcg actgtgtgaa
    43261 tatatttcgc cacgcactct ttgcccgcgc cggtttcgcc ctgaatgagc accggcacgt
    43321 taaatgcggc gacacgcttt gccagagtaa aagcgttaat gctggaagcg gcagttgcaa
    43381 taagttcaga catagggtga acgatataag tagcgacaat aatcaggatg ttctgaagcg
    43441 tatcaccggc gggattaaca agttcgaatt aaacgataat aaaaacatta tttgtttata
    43501 aacaatgatt tatccatgta ggccggataa ggcgtttacg ccgcatccgg caattgtgca
    43561 ccggtgcctg atgcgatgcg gcgcgtctta tcaggcctac ggttcgtgca atgtgttatg
    43621 acgatgcctg atatctcgc
//
LOCUS       DAZFJF010000025        42968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFJF010000025 DAZFJF010000000
VERSION     DAZFJF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.025"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..1216
                     /locus_tag="ACHKAU_001544"
     CDS             134..1216
                     /locus_tag="ACHKAU_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO5530638.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1405..1788)
                     /gene="quuD"
                     /locus_tag="ACHKAU_001545"
     CDS             complement(1405..1788)
                     /gene="quuD"
                     /locus_tag="ACHKAU_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO5530639.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1874..2014)
                     /gene="ylcG"
                     /locus_tag="ACHKAU_001546"
     CDS             complement(1874..2014)
                     /gene="ylcG"
                     /locus_tag="ACHKAU_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO5530640.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2011..2373)
                     /gene="rusA"
                     /locus_tag="ACHKAU_001547"
     CDS             complement(2011..2373)
                     /gene="rusA"
                     /locus_tag="ACHKAU_001547"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO5530641.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2370..2660)
                     /gene="ybcO"
                     /locus_tag="ACHKAU_001548"
     CDS             complement(2370..2660)
                     /gene="ybcO"
                     /locus_tag="ACHKAU_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5530642.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2653..2823)
                     /gene="ninE"
                     /locus_tag="ACHKAU_001549"
     CDS             complement(2653..2823)
                     /gene="ninE"
                     /locus_tag="ACHKAU_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5530643.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2823..3278)
                     /gene="ybcN"
                     /locus_tag="ACHKAU_001550"
     CDS             complement(2823..3278)
                     /gene="ybcN"
                     /locus_tag="ACHKAU_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO5530644.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3275..3376)
                     /locus_tag="ACHKAU_001551"
     CDS             complement(3275..3376)
                     /locus_tag="ACHKAU_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530645.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
     gene            complement(3500..3901)
                     /locus_tag="ACHKAU_001552"
     CDS             complement(3500..3901)
                     /locus_tag="ACHKAU_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530646.1"
                     /translation="MGKSTLVIGRELTKDMEFSGQFLLKKLKLQNLTIDAAMWFYYPD
                     LSWRYILVISDFSERGPAEIYRKISEINRNSISKKYKPIPLEAIEAKGDSAFVYKMLK
                     GFARVNDGKVRVSNSMVNGLEIVDCLIYELK"
     gene            complement(3880..4296)
                     /locus_tag="ACHKAU_001553"
     CDS             complement(3880..4296)
                     /locus_tag="ACHKAU_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFO5530647.1"
                     /translation="MNKNDLEALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKAC
                     IAKSFLQHEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWS
                     EQFRYDNNISKAMAEQLFDAVGDQNSGVLKWVKAHW"
     gene            complement(4596..5204)
                     /locus_tag="ACHKAU_001554"
     CDS             complement(4596..5204)
                     /locus_tag="ACHKAU_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO5530648.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(5630..5698)
                     /locus_tag="ACHKAU_001555"
                     /pseudo
     CDS             complement(5630..5698)
                     /locus_tag="ACHKAU_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5957..6304
                     /locus_tag="ACHKAU_001556"
     CDS             5957..6304
                     /locus_tag="ACHKAU_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530649.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(6509..7210)
                     /locus_tag="ACHKAU_001557"
     CDS             complement(6509..7210)
                     /locus_tag="ACHKAU_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5530650.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(7207..8226)
                     /locus_tag="ACHKAU_001558"
     CDS             complement(7207..8226)
                     /locus_tag="ACHKAU_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5530651.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(8223..8762)
                     /locus_tag="ACHKAU_001559"
     CDS             complement(8223..8762)
                     /locus_tag="ACHKAU_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO5530652.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(8832..9062)
                     /locus_tag="ACHKAU_001560"
     CDS             complement(8832..9062)
                     /locus_tag="ACHKAU_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5530653.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            9167..9856
                     /locus_tag="ACHKAU_001561"
     CDS             9167..9856
                     /locus_tag="ACHKAU_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5530654.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            9938..10201
                     /locus_tag="ACHKAU_001562"
     CDS             9938..10201
                     /locus_tag="ACHKAU_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530655.1"
                     /translation="MSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTE
                     WPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            10214..10657
                     /locus_tag="ACHKAU_001563"
     CDS             10214..10657
                     /locus_tag="ACHKAU_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530656.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            11208..11414
                     /locus_tag="ACHKAU_001564"
     CDS             11208..11414
                     /locus_tag="ACHKAU_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO5530657.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
     gene            11490..11786
                     /locus_tag="ACHKAU_001565"
     CDS             11490..11786
                     /locus_tag="ACHKAU_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5530658.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            11792..12577
                     /gene="bet"
                     /locus_tag="ACHKAU_001566"
     CDS             11792..12577
                     /gene="bet"
                     /locus_tag="ACHKAU_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5530659.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            12574..13254
                     /locus_tag="ACHKAU_001567"
     CDS             12574..13254
                     /locus_tag="ACHKAU_001567"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5530660.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            13251..13433
                     /locus_tag="ACHKAU_001568"
     CDS             13251..13433
                     /locus_tag="ACHKAU_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO5530661.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            13406..13597
                     /locus_tag="ACHKAU_001569"
     CDS             13406..13597
                     /locus_tag="ACHKAU_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5530662.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            13674..13889
                     /locus_tag="ACHKAU_001570"
     CDS             13674..13889
                     /locus_tag="ACHKAU_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5530663.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            13988..14206
                     /locus_tag="ACHKAU_001571"
     CDS             13988..14206
                     /locus_tag="ACHKAU_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5530664.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            14254..14532
                     /locus_tag="ACHKAU_001572"
     CDS             14254..14532
                     /locus_tag="ACHKAU_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5530665.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            14504..14569
                     /locus_tag="ACHKAU_001573"
                     /pseudo
     CDS             14504..14569
                     /locus_tag="ACHKAU_001573"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            14731..15894
                     /gene="intD"
                     /locus_tag="ACHKAU_001574"
     CDS             14731..15894
                     /gene="intD"
                     /locus_tag="ACHKAU_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO5530666.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(15910..15986)
                     /locus_tag="ACHKAU_001575"
     tRNA            complement(15910..15986)
                     /locus_tag="ACHKAU_001575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15950..15952),aa:Arg,seq:tct)
     gene            16229..16861
                     /gene="fimZ"
                     /locus_tag="ACHKAU_001576"
     CDS             16229..16861
                     /gene="fimZ"
                     /locus_tag="ACHKAU_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO5530667.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(16864..17379)
                     /gene="sfmF"
                     /locus_tag="ACHKAU_001577"
     CDS             complement(16864..17379)
                     /gene="sfmF"
                     /locus_tag="ACHKAU_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO5530668.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(17390..18367)
                     /gene="fimH"
                     /locus_tag="ACHKAU_001578"
     CDS             complement(17390..18367)
                     /gene="fimH"
                     /locus_tag="ACHKAU_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5530669.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHDYLLGAMSLVD
                     SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTV
                     YVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRP
                     AGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            complement(18409..21018)
                     /gene="sfmD"
                     /locus_tag="ACHKAU_001579"
     CDS             complement(18409..21018)
                     /gene="sfmD"
                     /locus_tag="ACHKAU_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO5530670.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(21049..21741)
                     /gene="fimC"
                     /locus_tag="ACHKAU_001580"
     CDS             complement(21049..21741)
                     /gene="fimC"
                     /locus_tag="ACHKAU_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5530671.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(21961..22503)
                     /gene="fimA"
                     /locus_tag="ACHKAU_001581"
     CDS             complement(21961..22503)
                     /gene="fimA"
                     /locus_tag="ACHKAU_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5530672.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            22984..23850
                     /gene="folD"
                     /locus_tag="ACHKAU_001582"
     CDS             22984..23850
                     /gene="folD"
                     /locus_tag="ACHKAU_001582"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO5530673.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            23852..24064
                     /gene="ybcJ"
                     /locus_tag="ACHKAU_001583"
     CDS             23852..24064
                     /gene="ybcJ"
                     /locus_tag="ACHKAU_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO5530674.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            24172..24693
                     /gene="ybcI"
                     /locus_tag="ACHKAU_001584"
     CDS             24172..24693
                     /gene="ybcI"
                     /locus_tag="ACHKAU_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5530675.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(24729..26114)
                     /gene="cysS"
                     /locus_tag="ACHKAU_001585"
     CDS             complement(24729..26114)
                     /gene="cysS"
                     /locus_tag="ACHKAU_001585"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO5530676.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            26288..26782
                     /gene="ppiB"
                     /locus_tag="ACHKAU_001586"
     CDS             26288..26782
                     /gene="ppiB"
                     /locus_tag="ACHKAU_001586"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO5530677.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            26785..27507
                     /gene="lpxH"
                     /locus_tag="ACHKAU_001587"
     CDS             26785..27507
                     /gene="lpxH"
                     /locus_tag="ACHKAU_001587"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO5530678.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            27625..28134
                     /gene="purE"
                     /locus_tag="ACHKAU_001588"
     CDS             27625..28134
                     /gene="purE"
                     /locus_tag="ACHKAU_001588"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO5530679.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            28131..29198
                     /gene="purK"
                     /locus_tag="ACHKAU_001589"
     CDS             28131..29198
                     /gene="purK"
                     /locus_tag="ACHKAU_001589"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO5530680.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(29335..30228)
                     /gene="ybcF"
                     /locus_tag="ACHKAU_001590"
     CDS             complement(29335..30228)
                     /gene="ybcF"
                     /locus_tag="ACHKAU_001590"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5530681.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(30225..31040)
                     /gene="ylbF"
                     /locus_tag="ACHKAU_001591"
     CDS             complement(30225..31040)
                     /gene="ylbF"
                     /locus_tag="ACHKAU_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5530682.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(31051..32310)
                     /gene="ylbE"
                     /locus_tag="ACHKAU_001592"
     CDS             complement(31051..32310)
                     /gene="ylbE"
                     /locus_tag="ACHKAU_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5530683.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(32320..33987)
                     /gene="fdrA"
                     /locus_tag="ACHKAU_001593"
     CDS             complement(32320..33987)
                     /gene="fdrA"
                     /locus_tag="ACHKAU_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5530684.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            34303..35352
                     /gene="allD"
                     /locus_tag="ACHKAU_001594"
     CDS             34303..35352
                     /gene="allD"
                     /locus_tag="ACHKAU_001594"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO5530685.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            35374..36609
                     /gene="allC"
                     /locus_tag="ACHKAU_001595"
     CDS             35374..36609
                     /gene="allC"
                     /locus_tag="ACHKAU_001595"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO5530686.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            36620..37405
                     /gene="allE"
                     /locus_tag="ACHKAU_001596"
     CDS             36620..37405
                     /gene="allE"
                     /locus_tag="ACHKAU_001596"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO5530687.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(37534..38679)
                     /gene="glxK"
                     /locus_tag="ACHKAU_001597"
     CDS             complement(37534..38679)
                     /gene="glxK"
                     /locus_tag="ACHKAU_001597"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO5530688.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(38701..40001)
                     /gene="ybbY"
                     /locus_tag="ACHKAU_001598"
                     /pseudo
     CDS             complement(38701..40001)
                     /gene="ybbY"
                     /locus_tag="ACHKAU_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(40058..41419)
                     /gene="allB"
                     /locus_tag="ACHKAU_001599"
     CDS             complement(40058..41419)
                     /gene="allB"
                     /locus_tag="ACHKAU_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO5530689.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(41479..42933)
                     /gene="ybbW"
                     /locus_tag="ACHKAU_001600"
     CDS             complement(41479..42933)
                     /gene="ybbW"
                     /locus_tag="ACHKAU_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO5530690.1"
                     /translation="MEHQRKLLQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT        11260 a        10868 c        10206 g        10634 t
ORIGIN      
        1 aaaaatagag atctacttca caaatcaaat gagaaaccaa aacttacatc ttgaaataat
       61 cacattgatt agatgaatat ttatcgcgca gtgacatcat tttttaataa tagttcaaaa
      121 aaaagggctc acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcagtact
      181 gatggcgatg tctgctcagg cagctgaaat ttataataaa gacagtaaca agctggatct
      241 gtacgggaaa gttaatgcta agcactactt ctcctctaat gatgcagatg atggtgatac
      301 tacttatgcc cgtcttggct tcaaaggtga aacccaaatc aacgatcaac tgactggttt
      361 cggtcagtgg gaatatgaat tcaaaggcaa ccgcgctgaa tctcaaggtt cctccaaaga
      421 taaaacccgt cttgccttcg ctggcctgaa attcggtgac tacggctcca tcgattatgg
      481 ccgtaactac ggtgtagcat acgatattgg tgcatggacc gacgttctgc cagaattcgg
      541 tggcgatacc tggacccaaa cagatgtatt catgactggt cgcaccacag gtgttgcaac
      601 ttatcgtaac aatgacttct ttggtctggt tgatggtctg aactttgctg ctcagtatca
      661 gggtaaaaat gaccgtactg acgtaactca agctaatggt gatggtttcg gtttctccac
      721 tacttatgag tatgaaggtt tcggcgtagg tgcaacctat gctaaatctg atcgcactaa
      781 taatcaggtt atctacggta acaacagcct gaatgcatct ggtcaaaatg ctgaagtatg
      841 ggcagctggt ctgaaatatg atgcgaacaa catctatctg gctactacct attctgaaac
      901 ccaaaacatg actgtttttg gtaaaaacca tattgccaac aaagcacaaa acttcgaagt
      961 agttgcacaa tatcagttcg acttcggtct gcgtccgtcc gttgcttatc tgcaatctaa
     1021 agggaaagac ttgggtgctt ggggtgatca ggacctggtt gaatatattg atgtaggtgc
     1081 aacctattac ttcaacaaaa atatgtccac ttttgttgac tacaaaatca acctgattga
     1141 taagagcgat ttcacgaaag catctggcgt tgctaccgat gatatcgttg ctgtaggtat
     1201 ggtttaccag ttctaatttg attactaaaa catatgttgt gggaggcttt gcctccccaa
     1261 catataagtg gctccctcaa gccacttcct ttagaagcac aaccttgctt ctaactatat
     1321 aaaccttctg ttatatatta ccctttattt ttgggggcgt ttcaacgccc cattcttaat
     1381 aatttttagt aaacaattgg catattaatt agagttatta acaacgatat ccatctctaa
     1441 ccggatatct aatgccatta acatcccttc aattatgccc tcagccttct gtaacctttt
     1501 cccgatataa ccatcagagc agcaatgctt acctgccagt gacatgaatg tcataccgac
     1561 tacataataa tctactaata aatcgtgcaa atcgctgttg ttctttttca gacgggccat
     1621 gcacccgcaa atgatcatcg cgtcatcgtc acaacattgc gggcgagatt ttacttttga
     1681 agtaattaat cccttaaaac cggcggcaat ggacgaccag gtcacatctt catgattatt
     1741 agccgcccac gctccccaac gctcaagaac catctgaata tcacgcatca acttactcca
     1801 caaaaatcag accagaacgc caattacaag caaaaatcaa cgaaacagta ttagttgatt
     1861 gttatctctg acttcatact cctgctcctg tcagggtttt ggcgtaattc ttcagtattc
     1921 ggtaatcggt caaaacagaa ccggggaaac gatataagcg cagacgcccc cagcggtggc
     1981 gaagacgttc tgccatataa aactcaaaca tcattcattc cccatttcgg tgatggtcag
     2041 ttccagcctc ccacctttgg taacaggcat cttcacaacg cggtaatcaa cgacctgagc
     2101 atcatccagc cagaaacctg ctttagtgag tgcgtcaaaa gcggcttttt gcagattatc
     2161 caggtcacgg cgacggcgat ccggcatgtg gcactcaatg cggattttca caggcatagc
     2221 caagccgata tccagcattg cgtttttaat gattcgggcg acgttatcgc ggtatgcctg
     2281 cccctctgcg ctgacgtgcg tgcgcccgcg attatggcgg taatagcgat tattgctcgg
     2341 aggccagggt aatgtgatgc tgtaggtatt cacgccttga ttaccccctc tttcagccag
     2401 ataacctgcg ttctcgccat accttccagc gcgcattctt ttgcgtactc agcatcgaca
     2461 aaatgcgtgc ggcggtcgat ttcgtcgtga caggcagaac aggcaatggt ggcaataagg
     2521 tctggcggtt taataccggt gccacacagt ccagccagcc ggatatgtgc cagtacagac
     2581 gtttcagggt tgccattgca tacgccaggg attcttacct ggcattcgcg accacgcgct
     2641 gcttttctca aatcagccat gattcctcct tgctgccagt cgcaaccatt ttttatcaac
     2701 caggctggcg gtatatccga gcagtgttgg tatttcggat ggttttagct caggcttacg
     2761 cttacgacga tttggtactc tgtagatgtg tccgttcatg acacgaataa gcggtgtagc
     2821 cattacgcct cctgcttgtc gcgcagcagc tggaactcgc agctctgcgg aatagtcagg
     2881 tggcagccaa tattcatcgc ccaggcttca accttacaca ggaagacata catctctccg
     2941 gtatcaagat cggaggtatg gcgtaacgac tggatagtag tgatttcgcc ggttacgaca
     3001 tcaaccaggt ccttggtttc ataaccgagg tatgtgtgtt tgagagcatc ttttacccat
     3061 gctgcggtag cgaacgattt ccccctgcgg ataaggtatt cactgatttc gctgtaccac
     3121 atgtggctaa gtgcattctg ggaaagactg cgtctctcgc gccacggttt aagcaccatg
     3181 cgaaagcact tgccgtcttc caaataaggc tggagctgct gaccgatagc ggtgaagtta
     3241 ccgcgatgca gtttgatgcc gtcttgtggg aggttcacgc ttcacctccg cagaggtcaa
     3301 acgctggata aagaaaatcg caggtgcatt tctgcatctg tgacgggaga agatagttta
     3361 gattgtatgt gcgcataaac gtccccgttt agcgcagaag tcaccggagg agttcaaact
     3421 ccggtgactt aattatgaca agttgattat tgaaaatcaa aagtcattta aacaataatg
     3481 atacccagca agagatttct tattttaact catagatcag acagtcaacg atttctaaac
     3541 catttaccat agaattagaa acgcgaactt taccatcgtt gactctagca aatcctttta
     3601 acattttata aacaaaagct gaatccccct tagcctcaat tgcttctaat ggtatcggct
     3661 tatacttttt tgatatgcta tttctattta tctcactgat ttttctatat atttctgccg
     3721 gcccacgttc tgagaagtca ctgataacta aaatatatct ccaagataga tctgggtaat
     3781 aaaaccacat tgcagcatca atagttaaat tctgtaactt gagttttttt aacaaaaatt
     3841 gacctgagaa ttccatatct tttgtcaact ctctgccgat taccagtgtg cttttaccca
     3901 tttcaaaact ccagaatttt ggtcacctac agcatcaaac aattgttcgg ccatagcttt
     3961 acttatgtta ttgtcgtatc taaattgctc gctccagtct ttaacgaccg accagttaat
     4021 ctccaacgaa acatcatttt ttgcgtcagc aatcaaaatt tgatgtaagt tggcaatttt
     4081 gagaagttgc gacaaatcat gagtatatga atcatttacg acttttttgt ttggaaactc
     4141 atgttgtaaa aatgacttgg caatacaggc cttcaatgca cattcaactg catacccaca
     4201 aagataatat gcaccatgaa aaaagccatg atcaagcaag catttggcct cattaagcct
     4261 agtgtcagat aatgcttcaa ggtcgttttt attcatacaa ttataacttg aattatccta
     4321 atttttatga ttataccctt atcaggtatg atgtaggaac tgaaaacaat attggatatc
     4381 attttcggta tttctctgca aattccagcc cacaggatct cggtgtacaa tctgtcgctg
     4441 atcgcgcaaa tttagggtca atcacttcat tttttgcatc aaaccctcac taacatcgaa
     4501 cgattatagt acaagtagtg ttcttgactt ctcattaaac cgtcagcatt caattaatta
     4561 caaaatacta gcggcatttc tctcccgcaa ctttattaca gccgttcaga acgtaaatgc
     4621 gtcaatactc gactccactt atcatcctgc cacggccgga attttacatg tgccgtttct
     4681 ctggcaagga ttgcctgtgc cttattgagt atccggggat attcttgttc gattgaagtg
     4741 aagcgaccgg cttcgcggtg ttctgctacc tgaagaagcg gagtgacgtt ttggcaagcg
     4801 gttaacattg tgtcacttgc ccgccataac caagcaagtg tgcaaagttc gttatcagtg
     4861 aattgttttg tgattgggga ttgttgaatt tctcgatcga gaatatccag cacccagcgg
     4921 cggaattctt tggccacagg agtgcgggcg aacatggcga tcagatgggc gccacggagg
     4981 gagaaaatgc gagtcttctt acgataattt cctgaggtcg ttgattcgac gacctgagac
     5041 attccgtcag taaactcatc aatatttttg ttgtagatgt cggttactgc tttggtgctg
     5101 gcatatttta atgctgccgc caattctttg ctggtaaacc agatctggtt gttgtgattt
     5161 ataggagtga attttacatc gcggaatgcg agctgagtga ccatagcgat aatctcgttt
     5221 tgaatttgag ctatcaccac cgacgacgcc aatcgactgg tggtggactg tgcaaggttg
     5281 gcgtaaccgg tcaaaacgaa atccggcgct tccgaagaag ccctcacaca gcccaccata
     5341 atttttgtgt agacgtgcac tgcgcataaa aaaaccgcga acgcggttat gcgtcgtttt
     5401 gatgtccggg acgccaatcc cgtgtgccga ttttgcggca acgcacagaa tatagcgccg
     5461 gataaataat gtcgtcaacc cttgcatatg gggggcgcga tgatcactac taccaccata
     5521 atcgctccaa ctgttgcaac tactttaggc taagacatca cgatttccat atcaccttta
     5581 gccgccagac aaacagagac tccctgttct tggctgccaa aaacaccgaa tgcaaaaaat
     5641 cgcaggtgta tttctgcatc tgtggaggga gaagagagtt tggattgtat gtgcgcataa
     5701 acgtccccgt ttagcgcaga agtcaccgta gttgttcata ttctgatgac atgataattt
     5761 cctattgaat agaaaaaatt aacacgactc attcttatga ctggaaaaag caatcatgct
     5821 aataatcatt gttgttccag ttccaaatgc agcgaaataa tcaccaatat tttttctacc
     5881 ggtttcgact ttgatgctat attctgctaa cacctaacta acactacgtt tcacacagca
     5941 attacatgga aataacatga ctatcaaagc gactgacata gagattcttc atcgttatgc
     6001 tgaaggggta atggaacgtt ctaaccatca tgcaaaaaat gttggtgcag ccgctcttac
     6061 attgctagga ggtgttatct ggaaagccct gcccggatca atcgagataa gaacgtataa
     6121 cggaagtctt gcaaatatgg tgtggtggca aagcgaaaga acattaaaaa attatgcaat
     6181 ctcatacaac cataattcat gtgaaattga aatgagagat gaatcagtca aaggtgctgt
     6241 attatttagc atttccaacg aaaccactcc agaaaaaata ttatcacagc tttctgagct
     6301 ttaattaaag gcggtgctaa caccgcccat accattactc cacatcttac gcaacttgat
     6361 aattatgctc tattaaatac ccacaatatt tcatcaatgc atgggtcagc tacaaaatgt
     6421 ttaccgtggt aattcctgtt ttcgcaacat ctcaaaagaa caataaacac tgtatttctt
     6481 caagtaacga agaatctttc tttgcccgtc atacacttgc tcctttcagc ccaaacttag
     6541 ctttgatttc tgcgatcttc gccagagcct gtgcacgatt tagaggtcta ccgcccataa
     6601 caggaagttg ttttactggt tcaggtatcg tctcaccacg gttaattcgc gctgtcatac
     6661 aggtcagttc atcggcagcc ttgcgccgta attccgcgtc agtcagcgca ttggcccgca
     6721 tgttctggta caggttggta accaaccagt aatgcgcgtt cgatttccac ggataagact
     6781 ctgcatccgg atacaggcca cgcttccggc aatactcgta aaccatatca accagctcgc
     6841 tgacgtttgg cagcccggcg ttaacggatg cttcttcccg gcaccatgca acaaactgcc
     6901 cgggtgatgg cagaaatggt ctattctgcc gacgggcaac acgcattccg gcgctcacct
     6961 gttccatcgt ggtgatcccg ttttcccgga aagccagaac ccactggcgg cggatttcgt
     7021 tcagttcgtt ctggtcacga ttagccaggc tcgccgggaa agttgccagt aactggctga
     7081 atacaccgtt gattatctgc gctacctgct gtacctgcgg cttttcgtcg tactgttccg
     7141 gcatgttgtt ggcgatccga cgcatctgct cacggtcaaa gttaatcatc tgtgcggcga
     7201 tgtttttcat agatccaccc cgtaaatcca gtctgtgttt gtcaggtcga gttttggttt
     7261 gccgactgtc acgccagcct gttgcttgtt ccggttgatt tcgagctggg tccacttgtc
     7321 gcggagtttg gccggactca gcacgttacc ggaccagaag ttgtcctggc aggcccagcg
     7381 gaacagtaca cacatgtcgc ggtggttacg tccatcacgt tcacgcatca gacggatatc
     7441 gttagcccac cctgcaaaat tcggttttct ggctgatggc gcgatggtct tcaccatgtc
     7501 aaacatccac tctgcggcgg tcaggtcttc tgctgtcccc cacttgctgc cgctctgaat
     7561 cgcagcatcc gctttcacca caggaaggtc gttttctggc aggtcagagg attcgccaga
     7621 attctcggac gaataaggtt ttatattgtc ttttgttagt ttgtcttttg tgtttacctg
     7681 attcgggtaa gtgcctttac ctgatttggg taaacttttc ttacctgatt caggtaaatt
     7741 tacctctttc aggtaaactt tatttttctt acctgattcg ggtaatgttg accattcact
     7801 gaccacatta ttaatgccga tattccgccc gctctgaata aaaatcccac gctttaccag
     7861 aacacttttt gcagcagaac acttgtgcgg caatatcccg gtcaactcgg aaagttgctc
     7921 gttgctcacc cagtccagtt ttttattaaa gccatatgtt ttgcgcatga cagccaggaa
     7981 gaccagaagc tggtgctgtg ttaatccggc cagcattaca gcttccagca actcatttgc
     8041 aatgcgcgta taaccatcat cgagatctgc cacgcgcggc tccttttgtg ccgcatccgg
     8101 cactggaaaa ttgaatatct cagcagtgtt tgccataatt cctcccgcaa tgagtgtgtt
     8161 acgatttgca cctgaaagtc ggttctgttc cagcagaccg gctttcgcca tttctgaacc
     8221 tgtcatatcg cccccagcat ggtagtaacc atcgccatca atggaccagc cagctctggg
     8281 tccacacgaa acatcgacac aataccttca ctaatttcct tcagtttctg gtggcgtggt
     8341 gcgttgagaa tgacagcctg ttttgcctca ctgagttcct tttccatttc agccaaccta
     8401 gccatgaagc tatcctgctc aaccaggtaa ccgcgatatt ccagcggtag taccgccaga
     8461 attgccgggg tcagttcacg cacgttattt cggtattttt cagaatcgaa tttgttatcg
     8521 aggaagcgga acagcttctg gcgtgtacgg ctgacatcat cagggaaatc gatggtgccg
     8581 ccgccctgct cccgatactc attcacaatg agtgtggcaa cgacatcctg attatctaca
     8641 gccgaccagg cgcggacggc atcacggatt ttttcgtggc ctggcacctg ttttgtttga
     8701 gaacgattta tcaccgcagt cgggctaaat ccgctagtct gttggtatgg aagtggttgc
     8761 ataattgact cctttagttt gaattgactg ttaagttgat tgcttattgt taaagagcgt
     8821 gaaatggaaa tttaagctgc gttcttttcg gtgtgtggaa acaacttcgg aagatccggg
     8881 cgaatctggt atgccttcac tactccacca gtagccgtaa caatgctgcc gacatgttca
     8941 ggggatacct ttgctttgtt gtgaagccac ttatagacgg cctgctgtga aacttcgcag
     9001 gcagcgccca gtttcttttg tgaaccaacg atattgatcg ctgttttgat agctgggttc
     9061 ataacaacct ccgtggttaa tttgaatcaa gattaaaact atggttgttt ttagtcaaca
     9121 accattttcg tttgatggaa taaaaccttg gttgtacatt tggactatga aaacaacact
     9181 ctcagaaaga cttaaagaag ccagattagc gcgaggcctt acacaaaagg cgcttgggga
     9241 tttggtcggg gttagccagg ctgctattca gaaaatcgaa acagggaaag ctaatcaaac
     9301 aactaaaatc gtggagatcg cgaacgcttt gggtgtgcgc gcagaatggt tatcttctgg
     9361 cgttggaaat atgtcagaca gtacagtgca accaatacaa tcaactgtca gccattccaa
     9421 atacttcaag attgacgttc ttgatataga agtcagtgct gggccgggag tcatcaaccg
     9481 tgagtttgta gaagttctac gctcggttga gtactcgttt gacgatgctc gtcacatgtt
     9541 cgatggtagg aaggcagaaa atatccgcat cattaacgtg cgtggtgaca gcatgtcagg
     9601 aacgatcgaa ccaggtgatc tgctgttcgt tgatatcacg gttaaatctt tcgacggtga
     9661 tggtatctat gcatttctgt acgacgacac cgcccatgta aagcgcctgc aaatgatgaa
     9721 ggataaactg ctggttatct ctgataacaa gagctactca ccgtgggacc ctatcgagaa
     9781 agacgagatg aaccgggtat tcatattcgg taaggttatt gggagcatgc cgcagacgta
     9841 cagaaaacac ggataatcag ccgcgtgttg atgaggcttt tgggtaatac gctgaagaaa
     9901 ataccttata aaaaataaca ttacgggaaa ggcaaaaatg agtaataaaa cacttgttaa
     9961 aaaatcaaat aactgtgtcg atgcatacgc cgacgcgttt ggctactctt cgtttggtga
    10021 cggctctcag cgcctaggtt caatttcttt ttttcataat gtaactgaat ggcctgttga
    10081 tgaaagtgac gatgttgaga acataaagta caacattgca accattagaa tgcccgaaga
    10141 attgatgctt aaattggctg acttcattcg cgaccagcat gacaaagcta aaacaaacta
    10201 atcgtaatta attatgaaac acgattatta cgaagctcca aacctaaatg ataaaattgc
    10261 aaaggcggca gaatcatttt gtttgcactc cagtgcgtct tcccagctag caggtatatc
    10321 tggaaaaaga tgggacatgc tacaatcaag tggtagcagt gggtctggga gtgataacat
    10381 gcctgaaaaa gttgcaaaac tggagtccga tgttgctcat atcaaacgag acgttgatga
    10441 acttaaaaca gacgttaaag ctatcgacaa aaatatgata actattcttg cacgtctgga
    10501 ctctataaaa gaatccttaa caaacaagcc atcaagtgat gctgtcgata gaaagatttc
    10561 agacgcaaag cttgcggtat tgcttggtgt tccagcaatc atcgctgcag gaacaggact
    10621 ttataagcta tcaatgtact ttttctttag tgcttaagaa aatgctcgca cccttgcatt
    10681 aggtaaagcg atttttcatt ccccattacc ctcgctctta accaatcatc taaacccggc
    10741 caccgtgccg ggttttcttt tgccctcccc tcatcataca ccgttcaaaa aaaccaccac
    10801 gacctcgctt cagttatcgc tatgcgatgc aagtcacaaa ataaatccat cttaaataca
    10861 accagttata tctaaaacaa ccaacaaaac aacttttgtt gttgacgata aaacaactat
    10921 agttttaaat gagttcatcg caacaacaca acgatacggc aatcacctga ttcaccgttg
    10981 cgatgaccgc ttagatccgc agcttgaatt tcagcaggct tcggggagtg cgagggatga
    11041 aacggacgcg tgaacgtcgg tgtgaccagc tgaaatcaac tcaacatttc ataccttagt
    11101 cgcttcaacg aggcggctta gttatgacaa ccggcggcca tccaccgcct gaatacgcgc
    11161 agaagtcttt atatgttcag cagcccagct tacgggcagg agtttttatg gttcatcaac
    11221 attacggaac gcagaccgtt aatcgcggtg cggtcatgcc aggaatgctg gtcaaacgca
    11281 aagatggtac ctggactgca tcagctaatt tacgcggacg actttatctg catcgcggca
    11341 ttgagcgcac ttatacccgt gacttgctcg tggaagtttt tctcgacgga cgcggcaacg
    11401 ctctgaatca ctaatcccct ttcctgtttt ccgaatcagc ctggcattcc gcgggcgatt
    11461 ttttcacagc cattttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg
    11521 aggctcagag ctgggcgcgt cactaccagc agatcgcccg tgaagagaaa gaggcagaac
    11581 tggcagacga catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc
    11641 atttgcaacg ccacggggcc agcaaaaaag ccattacccg tgcgtttgat gacgatgttg
    11701 agtttcagga gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc
    11761 aggttgatat tgattcagag gtataaaacg gatgagtaca gcactcgcaa cgctggcagg
    11821 gaagctggct gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct
    11881 tcgccagacg gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat tgttgatcgt
    11941 cgccaaccag tacggcctta atccgtggac gaaagaaatt tacgccttcc ctgataagca
    12001 gaacggcatt gttccggtgg tgggcgttga tggctggtcc cgcatcatca atgaaaacca
    12061 gcagtttgat ggcatggact ttgagcagga caatgaatcc tgtacatgcc ggatttaccg
    12121 caaggaccgt aatcatccga tctgcgttac cgaatggatg gatgaatgcc gccgcgaacc
    12181 attcaaaacc cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat
    12241 gttacggcat aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta
    12301 tgacaaggat gaagccgagc gcattgtcga aaatactgca tacactgcag aacgtcagcc
    12361 agaacgcgac atcactccgg ttaacgatga aaccatgcag gagattaaca ctctgctgat
    12421 cgccctggat aaaacatggg atgacgactt attgccgctc tgttcccaga tatttcgccg
    12481 cgacattcgc gcatcgtcag aactgacaca ggccgaagca gtgaaagctc ttggattcct
    12541 gaaacagaaa gccactgagc agaaggtggc agcatgatac cggacattat cctgcagcgt
    12601 accgggatcg acgtgagagc tgtcgaacag ggggatgatg catggcacaa attacggctc
    12661 ggcgtcatca ccgcttcaga agttcacaac gtgatagcaa agccccgctc aggaaagaag
    12721 tggcctgaca tgaaaatgtc ctacttccac accctgctgg ctgaggtttg caccggtgtg
    12781 gctccggaag ttaatgctaa ggcgctggcc tggggaaaac agtacgagaa cgacgccaga
    12841 accctgtttg aattcacttc cggcgtgaat attactgaat ccccgatcat ctatcgcgac
    12901 gaaagtatgc gcaccgcctg ctctcccgat ggtttatgca gtgacggcaa cggccttgaa
    12961 ctgaaatgcc cgtttacctc ccgggatttc atgaaattcc ggctcggtgg tttcgaggca
    13021 ataaaatcgg cttacatggc ccaggtgcag tacagcatgt gggtgacgcg aaaagatgcc
    13081 tggtactttg ccaactatga cccgcgcatg aagcgtgaag gcctgcatta tgtcgtgatt
    13141 gagcggaatg aaaagtacat ggcgagtttt gacgagatgg tgccggagtt catcgaaaaa
    13201 atggacgagg cactggctga aattggtttt gtatttgggg agcaatggcg atgacgcatc
    13261 ctcacgataa tatccgggta ggcgcgatca ctttcgtcta ctccgttaca aagcgaggct
    13321 gggtatttcc cggcctttct gttatcagaa atccactgaa agcacagcgg ctggctgaga
    13381 agataaataa taaacgggag gcggtatgca caaagcatct cctgttgagt taagaacgag
    13441 tattgagatg gcacatagcc ttgctcaaat tggagtcagg tttgtgccaa taccagtaga
    13501 aacagacgaa gaatttcata cgttagccac atccctttca caaaagctgg aaatgatggt
    13561 ggcgaaagca gaagcagatg agagagacca ggtatgacaa ccactgaatg catttttctg
    13621 gcagcgggct tcatattctg tgtgcttatg cttgccgaca tggggcttgt tcaatgacac
    13681 ctcagcaaga aaacgccctt cgcagcattg cccgtcaggc taattctgaa atcaaaaaag
    13741 ccagacagca ttttccggat aaaaacgtcg atgacatttg ccgtagcgta ctaaagaagc
    13801 accgcgaaac ggtaacgctg atgggattca caccgactca tttaagcctg gcgatcggca
    13861 tgttaaacgg cgtctttaag gaacggtgaa catgaaaagc aaaatcatca gggagctaca
    13921 ggctcctttt ttattgttcg cattcatcct caagcgtatt aaccaacaat tcagggatta
    13981 atggaagatg gcagacatca ttgattcagc atcagaaatc gaagaattac agcgcaatac
    14041 agcaataaaa atgcgtcgtc agaactacca gactgtatcc gcaactcatt gttgtgagtg
    14101 tggcgatccg atagatgagc gaagacgcct ggctgttcag ggttgtcgga cttgtgcaag
    14161 ttgccaggag gagatcgaac ttaagaacaa acaatgggga ctgtgatggc ctcaaagcag
    14221 caaatttcaa catcgtccaa ctgaggtgta aaaatgttca gaatcatttt tcctaacacc
    14281 tggtacgtcg accaccacgg cactccctgc aaaatcctgc gttctaccca caacaaagtt
    14341 cactacatcc gaaaaggcag aacatgtatc gccagcatgt tccgctttaa tcatgacttt
    14401 gaacctgtga ataaagctga tgcagatcgg atagcagaag agatcgaaac ggcagaacac
    14461 attaagaagt tacgtgacat gcgttcaaaa agcagaggta accatggaat catacagcct
    14521 cacactcgat gaggcctgtc agtttcttaa gatatccaga ccaaccgcca ccaactggat
    14581 acgaacaggc cgcctacagg caacacgtaa agatccaacc aagccaaaat ctccttacct
    14641 cacaacacgg caagcctgca ttgcggcgct tcagtctccg ctgcatactg tccaggtgag
    14701 cgcgggtgat ggcataacag aggaaagaaa atgtcactct tccgcagaaa tgaaatatgg
    14761 tatgcctcgt attcgctccc gggcgggaaa cgaattaagg aatctcttgg cacaaaggac
    14821 aagcggcaag ctcaggagtt gcacgacaag cgaaaagcag aactctggcg agtagaaaag
    14881 ctaggggatt tacctgatgt cacttttgaa gaggcctgcc taagatggct tgaggaaaaa
    14941 gctgataaaa aatctctcga ttcagataaa agccggattg agttctggct tgaacatttt
    15001 gagggtataa ggcttaaaga tatctcggag gcaaagattt actctgctgt aagcagaatg
    15061 cataacagaa agacgaaaga aatatggaaa cagaaagttc aggccgccat caggaaaggt
    15121 aaagaaccgc ctgtttatga accaaagcca gtatcaactc agacaaaggc aaagcatctt
    15181 gccatgataa aggccattct ccgtgctgca gaacgcgact ggaagtggct ggaaaaagcg
    15241 cctgtcatca agataccagc ggtcagaaac aagcgagtca gatggctgga aaaggaggaa
    15301 gcaaaacgcc ttattgatga gtgccccgaa ccactgaaat ctgtcgtcaa gtttgcgctg
    15361 gcaactggtc tgagaaagtc gaacatcata aatctggaat ggcaacaaat cgacatgcag
    15421 cgacgagttg cctgggtgaa tccagaagag agcaaatcaa accgcgccat tggtgtggcg
    15481 ctgaacgata ccgcctgtaa agtgttgcgt gatcaaatag gcaagcatca caaatgggtg
    15541 tttgtacata ccaaggcggc taagcgagca gatggaacat caacgcctgc ggtcaggaag
    15601 atgcgcatcg acagcaagac atcatggcta tcagcttgtc gtcgtgcagg aattgaagat
    15661 ttccgtttcc atgacctcag acacacctgg gcaagctggc tgattcagtc aggcgtccca
    15721 ttatcagtgc ttcaggaaat gggcggatgg gagtccatag aaatggttcg taggtatgct
    15781 caccttgcgc ctaatcattt gacagagcat gcgaggaaaa tagacgacat ttttggtgat
    15841 aatgtcccaa atatgtccca ctctggaatt atggaggata taaagaaggc gtaactgatt
    15901 gaattgtaat ggcgcgccct gcaggattcg aacctgcggc ccacgactta gaaggtcgtt
    15961 gctctatcca actgagctaa gggcgcgttg ataccgcaat gcggtgtaat cgcgtgaatt
    16021 atacggtcaa cccttgctga gtcaatggct tttgatctgg ttgctgaaca agtgaacgac
    16081 cgcgtctgat tttctgattt atttcgctat agcggcaaac aaacacacac cgctgcgcgt
    16141 ctgaatcaag aaaacccgta ttttcatgta tcaaagtaca atttaccgcc ctaacggaaa
    16201 attgtccgct cctatgagac tggtaactat gaaaccaacg tcggtgatca ttatggatac
    16261 tcatcctatc atcagaatgt ctattgaagt tctgttgcaa aaaaacagtg aattgcagat
    16321 tgtcctgaaa acggatgatt accgcataac catcgattat ctccgaaccc gtcctgttga
    16381 tttaatcatt atggatatag acttgcccgg aacagacggt tttaccttcc tgaaaaggat
    16441 caaacaaatc cagagcacag tgaaagtgtt atttttatca tcgaaatcag aatgctttta
    16501 tgctggcaga gcgatacaag ctggtgctaa cggttttgtc agtaaatgca atgatcagaa
    16561 tgatattttt catgccgttc agatgatcct ctccggatac acgttttttc ccagcgaaac
    16621 gcttaactat ataaaaagca ataaatgtag tacgaatagt tcaacggtca ctgtgctatc
    16681 taatcgtgaa gtgaccatat tacgttatct ggttagcgga ttatctaata aagaaattgc
    16741 cgataagtta ttacttagca ataaaacagt tagtgcgcat aaatctaata tttatggcaa
    16801 gctaggtttg cattcaattg tagagcttat cgactacgcc aaattatacg aattaatata
    16861 atattaatta taattgatca taaatatcgc atccgctttc gccacaccag gccgaacacc
    16921 gctggctaac gctcgataat tggcaaaaaa gtttagtgtg acattgccat ttgcatctac
    16981 ttcctcagtc gggctcgcct cccccagtgc gagccgggag cgatcgctat tacgtaattc
    17041 gatggcgacg gtttgtgcca ttgcgggatc atccagagcc agcaggttgg tatcggatgc
    17101 cggcgttccc gtaaataaaa tcgcaactga acccggagga catccctcca gccgcaggct
    17161 aaaagggacg agtgccgtgg tatcgccagc gttcagtaat tgtgtcgtag gccatctgcc
    17221 taaatctacc gtcttatcaa tatccgctgt gtttacggta caggagaaat caacaacgtt
    17281 accgtgcaaa ttgatattaa tcgttcctaa agggtcagct gcccatccaa tggaactcca
    17341 cagtaacccg cagaaacagc taaagagtac tcttctcatt ctccgtacct cattgataat
    17401 cgacgcgtaa ataccccagc gcgctaaacg gcccctcggt cggtttttga ccggtaatac
    17461 tgataggcca ggcgcgaagt gtgacattgg ctgccgcagc tgcatccaga cggaaaggaa
    17521 taacgctatt gagatcgtta ggcgtaatcg gcgtatcgtt ctgatcggcg acaataaaac
    17581 ctaaatcctg attgtccgac accatcgcct gaccagaaac ggcactggct tccagacgca
    17641 ttgttaaata agcctgcgca gcaacattcg tacatttgac cgcaatgctc ttggtttgcg
    17701 gcatgacgcc agctggtcga ttccccggcc ctgccgcact aaataacgat gcgccgatat
    17761 caccaaaatc aaattcaaca atctgcccgg catttaattc gcagttttgc ggcacttcaa
    17821 cccggccacc aaaactaatg gtataaacag gtgtaaccaa cggatcgtaa ggcgttgatg
    17881 tcacatacac ggtaaacatc acctgtctgg ggatctccac catgttaata aagggacgaa
    17941 taacctttaa acgaaatatt aaacgagaat ccatcacccc gaatggaagg ttttgcgaaa
    18001 cgttaggatc tgttcccatc cgaatataat tttgcggggg gaactgaata tccatcacac
    18061 tatcaaccag actcatcgca cctaatagat aatcatgtaa ttgcatatat ttataattat
    18121 cgatagtttc ctgaacaata aagttggtga cataactacg gtatgtataa ttcaccagcg
    18181 taccgggtgg acaaattgct gagacaccta cccagccgga tttttccggt aaaacaacaa
    18241 tctgccctgg ctggttattg gtgctattaa agatatttga gagatcatag tgttcatctt
    18301 ctgctacgcc agtactattt gcacaaacag tagcccagga tatgtttgcc agacacaaac
    18361 atgccatcgc caataataat ctgcagatta tcttattgat aatttccttt atgagccagg
    18421 atgtgtgcaa actgccgaaa taacagtgac ggcttgctgt aagctttgct tcggcaatac
    18481 atagtgcgca atgcagcgtg agttcgcgcc gtctccccac tgaaccagta attcaccaga
    18541 caatggcgca ccacttaaat aaacttgccc gtcatcaccc accatactgg ttattccggt
    18601 actgttttcc cgtaccagtg atccaaacgg caccggtttt ctgccctggg taacggtaat
    18661 gagcgcccgc acgcctatgc gggtatcaaa attggcacga accaacgcgc cttgcgtcgg
    18721 cacaacgctg ctaatatttt tttcaacatc gatggaattc cccatcgtat tggtatcaag
    18781 cgcgatacgg ttataccgat aaaccgtggc atacggcatc accgcatagc cgcgccagtc
    18841 ggttaaaatg ccagtttgat tttcaatgcg tacaccgcct gcgccaggcg ctttaatcaa
    18901 aacattggta tcccctaaag gctggctcag cgttatgcca ttttcatgcc cgaccacacc
    18961 gccagacagc tgccagttaa cgtcatgttg atcgcggtcg tagttatacc cgacccccag
    19021 cgtaccgtaa gcggcctgcc agtttgccgt ggcgctgccc gtatacccat tattcgaggt
    19081 atcaccctgg cttacgtgat aactcaggtt gtggccttcc agtaaggtcc cacctacacc
    19141 tgccagccag ctattttgcc cgttgctgtt acggttggcg ttaaaggagg cataagcgcg
    19201 gtcgagcgca ttttcccggg tgtagcgacg tttggtcaga acattgaaag gcactgaaac
    19261 attaagtccg acaatacgtt cgttatcggg gatccctaca gattcattcc acgaaaatga
    19321 gagtgaataa ctgatgccaa cccagctgct ggtatacccc acctgatacc aggtatctga
    19381 atccgaagta ttccagtatt tttgatgggt accagagata taaagcgagc caaagtcatt
    19441 aagtgattgt gaaatattta actgcaaacg gtctttacgg ctaaagcgta aattgtggta
    19501 attcacgatt atcggttcat cgcgatgctc accgtcgtaa tcgtaatcat attcgtaccc
    19561 ctccattcga cgatacgcaa catcatcaag ggtataaaaa ccttgtgtcg aatagcggta
    19621 acccattaac tgaaaattgg tgccgaaggt gttcatcgat ttcgcataga ggaagcgaat
    19681 ggaatccccc tcatgacgac tgtcgtcggc taactgactg cgcgcatggg tcacatccag
    19741 cgacactgtg ccccagttcc cgaggttgcg ccccagccct aataaaaagg cggtgtaatt
    19801 ggcagataat tgcgtcccgc cgtaggcagt aaattcctgt ggtaaaccgc cgagtgccgt
    19861 accctgaaaa aagaaaggcg atgattgctg actattaccg ctacgaaaat cgcccgccgt
    19921 caggtcaaat ttgaaacgcc cttcgcgttg taaaattggc actgttgaat acggaattgt
    19981 gtaattctgc tgattaccat cgcgctcgtc gatggtgacg tccagatcgc catttgaaga
    20041 tgtcgggtgc aaatctgtaa tttcaaaagc gccgggggaa acatagcttt gatagataat
    20101 aaaaccattt tgccgaatcg ttagctgggc cgccgtacgg gcaatcccac gtactgttgg
    20161 ggcaaaccct tgctggctat caggatacat attatcggaa gagtaaagcc gtataccacg
    20221 aaatccaaca ccatcgaaaa tatcacttcc tgtattgccg tctcccatta ccagttcact
    20281 tttcagcgga ataatggcgc gctgtaccca ggtgccaata ttattccact gttctgaatg
    20341 atatccattt ccgcgaaaat agttccagga accattgttg cgtaaacgcc acgggccaat
    20401 attaatcccg ctgagctcac taaaaaaata gctatcgtta ccattacctc tgttaccggt
    20461 gaaattataa ttcaggagta aagcaggaat accttcatcc cactcttcag gcggaatgta
    20521 accgtgagcg ctactcaaca acgctatctg aggaatagtg atgttcaggc gcatcgctgc
    20581 aaaatcaaag ttaattgtgg catcaggtac agctttcagt aaattgatgc atttattgtt
    20641 ttgctgctgg gctaactcgg gaaatgcact gctattaagg ctaattcgtt caaggagtac
    20701 ctggttaaaa cagggcatta acccacctga tttatcacct gtattttctg ttgtcgattc
    20761 aaatacgata tcctgtgaac caatgaactc atcattacgc cagagatcta cccgatacac
    20821 gcccgcaggt tgatgattac ctctctcaaa gcgcgataaa tcagcaacgg atgtgccatt
    20881 ctctaataaa aatgccgggt tgaaataact ttccgcatag cttaaaaagg agggtaaaat
    20941 ggcaagagat gaataacaaa ttccggccag acaccaggta tacctctgcg gaatatccgt
    21001 agtagtgggt attttcataa ttagctctga tattgtcaga tgattcggtt agtttaagtt
    21061 cacttctctg accggggtta ctgaaccata atcattaacg gtctgaaatt tcagctttcc
    21121 ctgcactcct gagggtaagg gaatttgtga attaactctg ggtgcagcca tagcattccc
    21181 caacttttgg ctgcccactt gtaagttaac cagagtgaca taaaaaggtg agggattatg
    21241 aacattgata tagttaccgg atcgcgaaaa cttgagtgtg tccggtgctt ctgcgggtaa
    21301 ttcttgtaat tgaattagac ggagaaataa tttcatgcgc gataaaatcg ccagttgcaa
    21361 aacattcctg ccgttcaatg catttttatc taccgaaggg atcgtcttaa cattcatcca
    21421 gaacagagac tcacgatctg ctgccagcgg tggaccggtg taaataatac gcaaggtatt
    21481 ttcgcttttg ggttcactaa caaacagtgg cggtgtaatg atgaatgact tttcttttac
    21541 accgctgctg ttttcaatcc acgaattgac cagaaagcgc tcattggtat ggctatttct
    21601 aatccatacc gcagtctgtt tagcatcagc gggataaata atacgcgtgg cacctaatgc
    21661 gatccctccg gcagcatggg cgctggccga aatgattaaa taaaatataa tgagcatcaa
    21721 taactttatt ttagtcatca tagttaatac gccttgtgta ttcctgacgg caaaaataag
    21781 aaatataaag aaatgattgc tttggtttct tacaacacat cattcccttt tcatttaata
    21841 aagcggcatg atacaaagtg aacatgattt cgatttacat tgccaccata ccgcttcaga
    21901 caaccggtac attactttgt tataagacgt gatatataat tcaaaacaac gtggttttga
    21961 ttattcgtat ctcataataa aagtcgcgtc agcatttgct tgccctgctg atgcacttgt
    22021 accggtgcct ttataacgtg cagaaaaatg aagaacgttg gtcccgggga tcaagttctg
    22081 gttggttgag aagctattcc catcaggttt gagaattgcg gatgtattat caagtatttc
    22141 aataccgaca cccgttgctg ttgttgtatt ggtactggat gcaatggcca ataaattatc
    22201 attgattgca tcagcctgac cagaaaatgc aacggcagca ttagcggcaa caacaggatc
    22261 gcagtcgttc aactgaatgg tgaatggaat taatgcagag gtattaccaa cagcattgaa
    22321 aatatcggta cgatattgac cgagtgtgac aacctggtct gccgagtcag tattcactga
    22381 acaggcagca ttcaccagtt cgccttcaaa gtgaattgta ccgccatcta caacggcagc
    22441 ataactaccc gtagcggcga atagtgcggc agccagcgca gacgaaataa atcttaattt
    22501 catatatatt ccttcaatct catttatcga ctccacatcc gtatataacc gattacttta
    22561 tttaagacac tgatagtagt aaaattcctt tttatcctct aagaatgtct taattgaaaa
    22621 tatgcactct attctaaaaa atagagagcc ccgttagatg aatactttcg cgcaaaatat
    22681 attcaacaca aatatagacc tgaagcggta aattaccagg ctgaaaattc tttttatatt
    22741 gtcaggtatt tcttaaatta tcttaatcct taaacaagga aataaatcag ttccagattt
    22801 acaacgccat catggacgaa aaatgaagct ttcaatctca gcgacctcag cgacggtgcg
    22861 cctcaccttc gcaagaggtc gcttcacgcg ataaatctga aacgaaacct gacagcgcgc
    22921 cccgcttctg acaaaatagg cgcatcccct tcgacctacg taacagatgg aatcctctct
    22981 ctgatggcag caaagattat tgacggtaaa acgattgcgc agcaggtgcg ctctgaagtt
    23041 gctcaaaaag ttcaggcgcg tattgcagcc ggactgcggg caccaggact ggccgttgtg
    23101 ctggtgggta gtaaccctgc atcgcaaatt tatgtcgcaa gcaaacgcaa ggcttgtgaa
    23161 gaagtcgggt tcgtctcccg ctcttatgac ctcccggaaa ccaccagcga agcggagctg
    23221 ttggagctta tcgatgcgct gaatgccgac aacaccatcg atggcattct ggttcaactg
    23281 ccgttaccgg cgggtattga taacgtcaaa gtgctggaac gtattcatcc ggacaaagac
    23341 gtggacggtt tccatcctta taacgtcggt cgtctgtgcc agcgcgcgcc gcgtctgcgt
    23401 ccctgcaccc cgcgcggtat cgtcacgctg cttgagcgtt acaacattga taccttcggc
    23461 ctcaacgccg tggtgattgg cgcatcgaat atcgtcggtc gtccaatgag catggaactg
    23521 ttgctcgcag gttgcaccac taccgtgact caccgtttca ctaaaaatct gcgtcaccac
    23581 gtagaaaatg ccgatctgtt gatcgttgcc gtcggcaagc cgggctttat tcctggtgac
    23641 tggatcaaag aaggcgcaat tgtgattgat gtcggcatca accgtctgga aaatggcaaa
    23701 gttgtgggcg acgtcgtgtt tgaagacgcg gctaaacgcg cctcatacat tacgcctgtt
    23761 ccgggcggcg ttggcccgat gacggttgcc acgctgattg aaaacacgct acaggcgtgc
    23821 gttgaatatc atgatccaca ggatgagtaa catggcgaca ttttctttag gtaaacatcc
    23881 gcacgttgag ctgtgcgact tgctgaaact ggaaggctgg agcgaaagcg gcgcgcaggc
    23941 gaaaatcgcg attgccgaag gccaggtgaa agtcgacggt gcggttgaaa cgcgcaaacg
    24001 ctgcaaaatc gtcgccggtc agacagtgag ttttgcaggt cacagcgtac aggttgttgc
    24061 ctgatccctt tcccggcctg tatcaacagg ccggttctct tttcagactt ttgccctcta
    24121 cgccccgttc atccatgctg atagcatcga cattttgccg aagcgaccat catgccaacc
    24181 gttattacgc acgccgccgt tcccctatgc attggtttag ggttgggttc gaaagtcatc
    24241 cctccgcgtc tgttatttgc cggaatcatc ctggcgatgc tgcccgacgc cgacgtattg
    24301 tcgtttaaat ttggcgttgc ttacggcaat gtttttggtc atcgcgagtt tacccattcg
    24361 ctggtgtttg cgtttgttgt cccgctgtta tgtgtgctga ttggacgccg atggttcagg
    24421 gctgggctga ttcgctgctg gctgttttta accgtctcgt tgctatcgca cagcttgctg
    24481 gattcggtaa ccactggcgg taagggcgtt ggctggctgt ggccgtggtc agatgaacgc
    24541 tttttcgctc cctggcaggt gattaaagtc gcgccgtttg cattgtctcg ttacaccacg
    24601 ccgtacgggc atcaggtgat tatttctgaa ttgatgtggg tgtggttgcc ggggatggtg
    24661 cttatgggaa tgttgtggtg gcgcaggcga taaccggatg cgaaaactcg catccggcaa
    24721 cagcgcaatt acttacgacg ccaggtggtc ccttgcgggc catcttccag cacgatcccc
    24781 atctcgttaa gacggtcacg cgccgcatct gccgccgccc agtctttcgc tttacgggca
    24841 tccagacgct gttgaattaa cgcttcaatc tcagccactt cgctgtcgtc tgcctgcgca
    24901 ccgctttgca ggaacgcttc cggttcttgc tccagcaggc ccaatacagc ggaaagttta
    24961 cgcaggtgag atgccattgc attcgccgct gccatatctt ctgctttcag acggttcact
    25021 tcacgcgcca tatcaaacag cacggaatag gcttccgggg tgttgaaatc gtcgtccatc
    25081 gcttcaataa agcgcgcttc aaacgcttcg ccaccggcag gcgcaacggt tttatctgtg
    25141 ccgcgcagcg caatgtagag gcgctccagc gccgcacgcg cctgcttcag gttctcttcg
    25201 ctgtagttca gttggctgcg atagtggccc gacatcagga agtaacgcac ggtttccgcg
    25261 tcgtagtatt tcagcacatc gcgcacggta aagaagttac ccagcgattt ggacatcttc
    25321 tcgcggtcaa ccatcaccat ccccgagtgc atccagtagt tcacatactg gccatcatgg
    25381 gcacaggtgg actgcgcgat ttcgttctcg tggtgcggga acatcaggtc tgaaccgccg
    25441 ccgtggatat caaagtggtt acccagctgc ttgcagttca ttgccgaaca ttcaatgtgc
    25501 cagcctggac ggcccgcgcc ccacggagac ggccagctcg gttcgccctc tttcgacatc
    25561 ttccacagaa cgaagtccat cgggttgcgt ttgtcgtcga ccacgtcaac gcgcgcacct
    25621 gcctgcagct ggtcgagatc ctgacgcgac agcacgccat atgtcggatc ggtcgggacg
    25681 tcgaacatca cgtcgccgtt gtccgccaca taagcgtgac ctttggcgat cagttgttca
    25741 gtgagttcaa taatttctgc gatatggtgc gtcgcgcgcg gctccatatc cgggcgcaga
    25801 atgttcaaag catcaaaatc tttgtgcatt tcggcgatca tgcgatccac cagcgccaca
    25861 aagctttcgc cattttcatt ggcgcgtttg atgattttgt cgtcgatatc ggtaatgttg
    25921 cgcacatact tcagtttata gccgaggaaa cgcagatagc gcgcaaccac gtcaaaagca
    25981 acaaaggtac gcccgtgacc gatatgacag agatcgtaaa cggtgattcc acacacatac
    26041 atgccgactt ccccggcgtg aataggctta aattcctctt tttggcgtgt cagagtattg
    26101 aagattttta gcatcgaaga ttccgtttag acatgtgtgg gtaattgagt tgcgtataat
    26161 acccatattt cccgcgcgaa tcagcataca ttgcgtgatg atccgatcgt gcggttatgc
    26221 tataacacca ccctatatat gacccgaact gggttgaagc accaatcaaa cggaacagga
    26281 tgcaaaaatg gttactttcc acaccaatca cggcgatatt gtcatcaaaa cttttgacga
    26341 taaagcacct gaaacagtta aaaacttcct ggactactgc cgcgaaggtt tttacaacaa
    26401 caccattttc caccgtgtta tcaacggctt tatgattcag ggcggcggtt ttgaaccggg
    26461 catgaaacaa aaagccacca aagaaccgat caaaaacgaa gccaacaacg gtctgaaaaa
    26521 tacccgtggt acgctggcaa tggcccgtac tcaggctccg cactctgcaa ctgcacagtt
    26581 cttcatcaac gtggttgata acgacttcct gaacttctct ggcgaaagcc tgcaaggttg
    26641 gggctactgc gtgtttgctg aagtggttga aggcatggac gtggtagaca aaatcaaagg
    26701 tgttgcaacc ggtcgtagcg gtatgcacca ggacgtgcca aaagaagacg ttatcattga
    26761 aagcgtgacc gttagcgagt aatcgtggcg acactcttta ttgcagatct tcatctctgc
    26821 gtggaagaac cggcgatcac cgccggtttt ctgcgttttt tagcggggga agcccgcaag
    26881 gccgacgcgc tgtatattct tggcgatctg tttgaagcat ggattggcga cgacgatccc
    26941 aacccactcc atcgccagat ggcggcagcg atcaaagcgg tgtccgattc cggcgttccc
    27001 tgttatttca ttcatggcaa ccgtgatttt ctgctcggca aacgctttgc ccgtgaaagc
    27061 ggcatgacat tattgccgga agaaaaggtg ctcgaacttt atggtcgccg ggtgttgatt
    27121 atgcatggcg acacgctgtg caccgatgac gcgggttatc aggcttttcg cgccaaagtc
    27181 cacaaaccct ggctgcaaat gctattcctc gccctgccgt tgtttgtgcg caaacgcatt
    27241 gccgcgcgaa tgcgagcgaa cagtaaagaa gccaacagca gtaaatcgct ggcgatcatg
    27301 gacgttaacc aaaacgcggt agtcagtgcg atggaaaaac atcaggtgca atggctgatc
    27361 cacggacata cccatcgccc ggcggtacat gaacttatcg ccaaccagca acctgctttt
    27421 cgcgtggtac tcggtgcctg gcatacggaa ggttcgatgg tgaaagtcac ggcggatgac
    27481 gttgagctga ttcattttcc gttttaaaaa acccgcaact ttgctgattt cacagccacg
    27541 caaccgtttt ccttgctctc tttccgtgct attctctgtg ccctctaaag ccgagagttg
    27601 tgcaccacag gagttttaag acgcatgtct tcccgcaata atccggcgcg tgtcgccatc
    27661 gtgatggggt ccaaaagcga ctgggctacc atgcagttcg ccgccgaaat cttcgaaatc
    27721 ctgaatgtcc cgcaccacgt tgaagtggtt tctgctcacc gcacccccga taagctgttc
    27781 agcttcgccg aaagcgccga agagaacggt tatcaggtga ttattgcggg cgcaggcggc
    27841 gcagcgcacc tgcctggtat gattgccgcc aaaacgctgg tgccagtgct gggcgtgcca
    27901 gtacagagcg ccgcactgag cggtgtcgat agcctctact ccatcgtaca aatgccgcgc
    27961 ggcattccgg tgggtacgct ggcgattggt aaagctggcg cggcaaacgc ggcgttactg
    28021 gcagcacaaa ttcttgcgac tcatgataaa gaactgcacc agcgtctgaa tgactggcgc
    28081 aaagcccaga ccgacgaagt gctggaaaac ccggacccgc gaggtgcggc atgaaacagg
    28141 tttgcgtcct cggtaacggg cagttaggcc gtatgctgcg tcaggcaggt gaaccgttag
    28201 gcattgctgt ctggccggtc gggctggacg ctgaaccggc ggcggtgcct tttcaacaaa
    28261 gcgtgattac cgctgagatc gaacgctggc cggaaaccgc attaacccgc gagctggcgc
    28321 gtcatccggc ctttgtgaac cgcgatgtgt tcccgattat tgccgaccgt ctgactcaga
    28381 agcagctttt cgataagctc cacctgccga ccgcaccgtg gcagttactt gccgatcgca
    28441 gcgagtggcc tgcggtgttt gagcgtttag gtgaactggc gattgttaag cgtcgcactg
    28501 gtggctatga cggtcgcggt caatggcgtt tacgtgccaa tgaaaccgaa cagttaccgg
    28561 cagagtgtta cggcgaatgt attgtcgagc agggcattaa cttctctggt gaagtgtcgc
    28621 tggttggcgc acgcggattt gatggcagca ccgtgtttta tccgctgacg cataacctgc
    28681 atcaggacgg tattttgcgc accagcgtcg cttttccgca ggccaatgcg cagcagcaag
    28741 cgcaagccga agagatgctg tcggcgatta tgcaggagct gggctatgtg ggcgtgatgg
    28801 cgatggagtg ttttgtcacc ccgcaaggtc tgctgatcaa cgaactggca ccgcgtgtgc
    28861 ataacagcgg tcactggaca caaaacggtg ccagcatcag ccagtttgag ctgcatctgc
    28921 gggcgattac cgatctgccg ttaccgcaac cggtagtgaa tagtccgtcg gtgatgatca
    28981 acctgattgg tagcgatgtg aattatgact ggctgaaact gccgctggtg catctgcact
    29041 ggtacgacaa agaagtccgt ccggggcgta aagtggggca tctgaatttg accgacagcg
    29101 acacatcgcg tctgaccgcg acgctggaag ccttgatccc gctgctgccg ccggagtatg
    29161 ccagcggcgt gatgtgggcg cagagtaagt tcagttaagc aggtgctcat ttgattgccg
    29221 gatgcggcgt aaacgcctta tccggcttac cgctccggta cccattgtag gcctgataag
    29281 acgcgtcaag cgtcgcatca ggcatggtgc accgattgcc ggatgctctc atcactacag
    29341 cgaaatacag gtccccgctt cgcccgccag cgtctcttca attcgcgata acgccccaat
    29401 ccacgcgggt ttaccacggc ttctgacata accacttacc gccgttacct tcggccccat
    29461 cgaaccatcg gctttggtaa atggcgctaa ctcatccggt gtggcatggc gaatggcgcg
    29521 ttgctgcggc gttccccagt tttcataaac cgcgtcagca tcggtgagga tcaccagtcc
    29581 atcggcatta atctgctcgg cgagcaacgc agcggcgaga tctttatcaa tcaccgcttc
    29641 actccctgcc ccatcttccg tcacaggcac accgccgccg ccactgcaaa tcaccacatg
    29701 cccctctttg agcaacaact cgatggcttc gctgtcgaga attttacgcg gttgcggaga
    29761 cgccaccacc cggcgcaaat atttaccatc acgtttcatc tgccagccgt aagccgcttc
    29821 cagtgccttt tgttcttctg gctgataaac cggaccaata aatttctctg gctgcaaaaa
    29881 cgccggatca tcaggcgaaa cctcaatgcg cgtcagcacc gtcgtgacag gcggcatctg
    29941 cggctgtgcg ctcaaactct gcgccagcat atagccaatc atcccctggc tttccgccac
    30001 cagcacatcc agcggatacg gatctacctc tttccacgcc agattctgca atgccagcag
    30061 ccccacctgc ggcccattgc cgtgaacaat cgccagccga taagaacggg ccaggcgcgc
    30121 cagcgcgggt acagcactgg cgatattgcg atattgattt tctgccgtca gcgcctcacc
    30181 gcgctggagt aaggcgttgc ccccaagagc tacaaccagt gttttcatgg ttttccttgt
    30241 aataattgtt ggccaagcca gaaccccagc agcgtatctg cccctgacgt atgccccagc
    30301 gccagcagcg aatcaatcgc aacagcggta cgcttcgggc aactcagagc atggacaaag
    30361 tgcaggagtg gcgaggcgaa atatccttgc gcggcataac gtaaataact gacgctcacc
    30421 gctgtggtaa cgagttgaag attgtcggaa caggcaaaaa acgggcgacc ggcgcgcgca
    30481 tctaaagcgc cataatacca ggccgccagc agcattccgc tcaacgtgtc atcatgactt
    30541 ggcgttaatc cggggccttt acccagccag tgccgccagt cggtcttaac gccattgagc
    30601 gcggcctgaa aacagtgacg aaactggcgt aattcagcgg gcagcggatc gcttgccgcc
    30661 atcgccagtg gtccgaaaag tccggtttcc tccgcgcgtt gcatccatgc agctgcaagc
    30721 ggttgaggat gcgaaggcgg cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc
    30781 gtgaaacgcc cgaggcgaat cccttgagcc acaacctgtg gtcgttcatt gccgcataat
    30841 ccaccgcata acgcatcgaa ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca
    30901 ctaccctgac gatgcagcgt taacagttcg ccgctttccg tcatcaggtt aatcgcccgt
    30961 cgccacactc ccgctaaccg ccaggactgg cgataattcg gtgcactacg gctggcaagc
    31021 agaggatgga tgatcgtcat tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc
    31081 ttccagcgcc tgttcaaaac acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc
    31141 gatccccgcc tctttatggg cgataccggt attgatgagt ggcgtaatgc cggtttctac
    31201 cacgcgacga atatccagcc ccaggcacgc gccctggaaa tcccagccgg ggatctgcaa
    31261 ctgcatattg cgctcaaggt aaatttccgc catctcttca gataccgctc tggccgcttc
    31321 catgccaccc gcaccgacaa agcgcgttac gccaggcgct gcgatcattg ccgcacctcc
    31381 gataccaaag gtttcggtaa tcgcgctatc gcccatatcc gggttcgcct gctcctgcga
    31441 gaagccggtg aaaaacagac cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc
    31501 cagcccgctt acccgaatcc cgaacatatt gccgttgcgg gtcattgccg tgacgatgct
    31561 gcctgcgcgg atcatcgcgc cagcatccat cgccgccttg cagtaagcca tcgcgaggtt
    31621 gaggaagaac tgatcggtca cgctgaggaa atccatcact tcggcgatgt gctgtttatc
    31681 atgatcgagg cgagcaattt gtggggccag cgcacgcatt aacagtgcag aggaagcaat
    31741 attgcgttga tggaactcat cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc
    31801 gataccgcgc tccatgcgcc ccagcgccgc gcttaatact ggcattaaca catcgcgcat
    31861 ccagcgatgg cgagtcagga catcttcgcc gtaagcgcca aaacgcatca ctttgccgat
    31921 accttcgttg aggttgcagt acgcccggtt gccgtcggtc acgttctcga ccaccagcat
    31981 cggcatactg gcagaagtaa taccgcccat tggaccgacg gcattcacat ggtgacaagg
    32041 aatgaagttc acttccccct gctccagtat tgccagcgcc tgcgcttcat ctttcgccca
    32101 gccttcgaac agacatgcgc ccacgcacgc ccctttcatg ggtccggtca tctcctgcca
    32161 gcgcattggc ggcccggcgt gaagcagtgt tttgccctcg tttagttcgc tgataagtga
    32221 agaagccggt tgcacatcca gccagtgtgg acgagcacga cgaatttgtt cgataaccgc
    32281 agcattggct tgcgccactg atgtaaacat gggaacccct tattgcaaac gttctaataa
    32341 acgagccagt tttttattgc cgccggcgac tggcgaccat tggtaatgca caaccggttt
    32401 gctggcgctt tgtagctcca gcgcaaagct gcgtaatccg atgttaatca cggcgacgtt
    32461 ttccagtaat gacggtgtgt gttgctgtgt ggcagacgag agcggatgaa ttaacgctgc
    32521 cgccagcaag gtggcttccg gtagcgaact cactaccgcg atccccgcat cttccagcgt
    32581 ggcgatttgc tgcgagcggc attgcgggtc acgttcggta cctgtcaccg tggcaatggc
    32641 gtacagcggt tggctgtctg aacgcgcggc acaggctttt tgccaggcgc tcaccagcga
    32701 ggcagcagga tcggcggtcg caccgaagcc aatcacgaca tcaagcagca acacgcgcac
    32761 ttgcggttta gcgccgagat cggcaattaa ctggttgcgt aaggctgggt cgatcatcgg
    32821 atggggacgc ccgacggtgt agaaatcatc gcccagatca atgatctggt gaccatcggc
    32881 gtccagcatc atgccgtgat gatgggtatc gtcggcttcc acgccaaggt gcccggcaag
    32941 taatcccgcc gcttcggcag caagcgtacc gccggtatac aaaccgcaaa taaatccgct
    33001 gctgacaggc gctattgcgt tacgtcgcgc cgtgacgcgt gaaagcaggc aagccaggcg
    33061 tgcggcctca tccagcgagg aggcaaacca gacattctcg tcgcgggcca ccgccggggt
    33121 ataacctaaa aacagcgcca ccgtcggttt gccggttgct ttcatggcat taacaatttt
    33181 cagacgcaca gcttcggcag gtggttttga aacaaatgcc agcacttcgc ttttctcgtc
    33241 tgcactgagc atttccagcg ctgttagcgc actgatgccg cccacttcac ggctgaggtc
    33301 gcgcccgcca aggccaatcg cgtgagtaat tccctcccct gccagcgcaa tctgcgaaca
    33361 cagctcctga atcccggtac cggaagcgcc aatgacgcca atattgcctt ctggcatcac
    33421 gttagcaaaa gccagcggtg tgccggcaat catcgacgta ccgcagtccg gccccatcac
    33481 cagcaagcct ttttcccgcg cgcgggtttt aagttggatt tcatcttcca gcgtgacgtt
    33541 atcggagaac atcatcacgt tgaggttgcg atccagcgcc tggtttgtca gctccgccgc
    33601 atactcgcca gccactgaaa tcagcgccag attggcatcg ggtaatttct gacaggcgct
    33661 gtcccagcga cgcacctgcg tcaacgcctg gctgctgcct gacccctgcg ccagttgttt
    33721 tagcgcctct tcaagctgct gcataatcgc ctgcgcgatc cccgcatccg ccgcttcgct
    33781 acgaattgcc acgcaaatat cgttcggcgt ggcgttatta aaatcgtcat gccagaaacc
    33841 tgtggtatct aataacgctt tattggcggg cgtacccatc attacggaaa catcatcaac
    33901 attttctgat tcgctgagtt ttcgtgaaat aatcattaaa ctgaccgaat cctgaaaaca
    33961 ccctttttta ataaaggcgt ggatcataac aactccttaa aaattgcacc catggcaatt
    34021 gcacgagcag tgttaatgtg gcgattacgg tatcgatgac aacgagtaaa taaggtgatg
    34081 aatatcacac agggattatt aacaaaagaa ttcaaataac cgccaggcta ttacgcatta
    34141 ttttttaata agcgtggttg ttttaaaaat caggttaaaa gcgcgaagat tatcaaataa
    34201 attctaacta tattttttgc ctgtctggat cacataatcc agatattttc cctgtatgtt
    34261 aattgcagtc atgcttcaca ccgtcgttaa aaaggaagac agatgaaaat cagtcgggaa
    34321 acactccacc agctaattga gaataaactc tgccaggctg ggttaaaacg tgagcacgct
    34381 gcaaccgtgg ctgaagtatt ggtttacgcc gatgccagag ggatccactc tcatggcgcg
    34441 gtgcgcgtgg aatactacgc ggaacgcatt tcaaaaggcg gcaccaaccg cgaaccggag
    34501 tttcgtcttg aggaaaccgg gccgtgctcg gcaattttac atgccgacaa tgccgccgga
    34561 caggtcgcgg cgaaaatggg tatggaacat gccatcaaaa ccgcccagca aaatggcgtt
    34621 gcggtggtcg gtatcagccg gatgggtcac agcggcgcaa tctcttattt tgtgcagcag
    34681 gcagcccgcg ccggattaat tggtatttcg atgtgccagt ccgatccaat ggtggtgccg
    34741 tttggcggcg cggaaattta ctacggtact aacccactgg cctttgccgc gccgggagaa
    34801 ggcgacgaga tccttacctt tgatatggcg actaccgtac aggcatgggg aaaagtgctc
    34861 gacgcccgct cgcgtaatat gtctatcccg gatacctggg cggtcgataa aaacggtgca
    34921 ccaacaaccg atccgttcgc ggtacatgct ctgctccccg ccgccgggcc aaaagggtat
    34981 ggcctgatga tgatgattga cgtcctctca ggcgtcttac tcggcttacc gttcgggcga
    35041 caggttagtt cgatgtatga cgatttacac gccgggcgta atttggggca attacatatc
    35101 gttattaacc cgaacttttt cttctccagc aaattatttc gtcaacatct tagccagacc
    35161 atgcgcgaat taaatgccat tacccccgcg cccggtttta atcaggttta ttatcccgga
    35221 caggatcagg atattaaaca acgcaaagcc gccgtcgaag gcatcgaaat tgttgatgat
    35281 atttaccagt atttaatttc cgacgcgctt tataacacgt catacgaaac gaaaaatccc
    35341 tttgcgcaat aattattgag acaggacttt cttatgatta cacattttcg tcaagccata
    35401 gaagaaacgc tgccctggct ttcctctttt ggcgctgacc caacgggtgg tatgacccgt
    35461 ttactttatt cgccggaatg gctggaaact cagcagcaat ttaaaaaaag aatggcagca
    35521 agcgggctgg aaacacgttt cgatgaagtg gggaatttat atggtcgcct gagtggcacc
    35581 gaatatccac aagaagtggt tctgagcggt tcgcatatcg ataccgtggt taacggcggt
    35641 aacctcgacg ggcaattcgg cgcgctggcg gcgtggctgg caattgactg gctgaaaacg
    35701 caatacggcg cgccgctacg tacggtcgaa gtggtggcga tggcagaaga agaaggcagc
    35761 cgcttcccgt atgtcttctg gggcagtaaa aatatctttg ggctggcgaa tcctgacgac
    35821 gtgcggaata tctgtgatgc caaaggaaat agttttgtcg atgcgatgaa ggcttgcgga
    35881 tttactcttc cgaacgcccc actaactccg cgtcaggata ttaaagcctt tgttgaactg
    35941 catattgaac agggctgtgt gctggaaagt aatgggcaat caattggcgt ggtgaatgca
    36001 attgtcgggc agcgtcgtta tacggtaacg ctgaacggcg aatcaaacca tgcaggcacc
    36061 acgccgatgg gttatcgtcg tgatacagtt tacgctttca gtcgcatttg ccatcagtcg
    36121 gtcgaaaaag cgaaaaggat gggcgatccg ctggttctga cctttggcaa agtagagccg
    36181 cgcccgaata cggtaaatgt ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat
    36241 accgacgctg ccgtgctgcg cgatttcacc caacagttag aaaacgacat gcgggcgatt
    36301 tgcgatgaaa tggacattgg tattgatatc gatttatgga tggacgaaga acccgtgccg
    36361 atgaataagg agctggtcgc caccctgaca gaattgtgtg aaagagaaaa actgaattac
    36421 cgggtgatgc acagtggtgc cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc
    36481 tgcatgattt tcattcccag catcaacggg atcagccata acccggcgga acgcaccaat
    36541 attaccgacc ttgccgaagg ggtcaaaacg ttggcactca tgctttatca acttgcctgg
    36601 cagaaataag gagtcataaa tgggatattt aaataacgtc accggttacc gcgatgattt
    36661 actggctaac cgtgcgattg ttaaacacgg taatttcgca ctgttaaccc cggatggtct
    36721 ggtaaaaaat attattccgg gctttgaaaa ttgtgacgcg acaatcctct ccacgccaaa
    36781 gctgggtgcc tcttttgttg attatctggt cacactgcat caaaacggtg gcaaccaaca
    36841 gggcttcggt ggcgaaggca ttgaaacgtt cctgtatgtg atctctggaa atatcactgc
    36901 caaagccgaa ggcaaaacat ttgccttaag cgaaggtggc tatctttatt gcccaccagg
    36961 ctccttaatg acgtttgtta acgcccaggc cgaagacagc caaatctttt tatataagcg
    37021 ccgctatgtt ccggtagaag gccatgcacc gtggctggtt tctggtaatg ccagcgaact
    37081 ggaacgcatt cactatgaag gtatggatga tgtgatcctg ctggatttcc tgcccaaaga
    37141 gttaggtttt gatatgaaca tgcatatcct ctcttttgcg ccaggtgcca gccacggtta
    37201 tatcgaaaca cacgttcagg aacatggtgc ctatattctt tccggtcagg gggtttataa
    37261 cctcgacaat aactggatcc cggtgaaaaa aggcgattac atctttatgg gcgcttattc
    37321 tttacaggct ggttatggcg tagggcgtgg tgaagcgttc agctatattt actcgaaaga
    37381 ttgcaaccgc gacgtagaga tttaagtcat accactcgcc tgattatttt tagctatgtt
    37441 ggattttgcc acggtttata ccgtggcttt ttttgtagta aattcaggta aaaaaagatg
    37501 cgaaataaat cgcatccgct ttaaaggtaa gggtcagttt ttaattccct gacctatttt
    37561 aatggcgcag gcaatatttc gcgcgctgtt gaagagattg gtttcaccgc tggcgagcac
    37621 ttcggctaaa ggtgccagac gaggcaaaat gctgaatacc gcgtcaatgc cgtactggtg
    37681 caccacttcc acgccatcac ccaatacgcc agcaatccca atcaccggga cattaaactg
    37741 cttcgccacc gacgcaacgc ccagcggcgc tttaccgcct gccgtttgcg agtcaatgcg
    37801 cccttccccg gtgatcacca gtgctgcgcc ctgcaccgcc tgctccaggt tgaccgcccg
    37861 cagtacaatc tcaatacccg gtttaatatc cgcattgagg aataccgccg ccgcaatacc
    37921 cataccgccc gcagcgccgc cgcccgccat ctggcagaca ttaatttcag tttgctgttg
    37981 taaaacacgg gcgtaatttt gcagcccctg ttcaagttct tcgaccatct ccggcgttgc
    38041 ccctttttgc gggccaaaca ccgccgccgc gccgcgtgcc cctaccagcg ggttatcaac
    38101 gtcacatgct acttcaatat ggcaattcgc cagacgcgga tcgcattcat ccatctcaat
    38161 gcttgccacg cgcgctaaat taccgccgtt agccgccagc acctggccgt cggcatcaag
    38221 gaaacgcaca ccaagcgcct gagccatgcc cataccgccg tcgaccgtcg cactgccgcc
    38281 aatgccgaga ataatatggc gaatgccgtt atccagcgca tgacgaatta actcccccgt
    38341 accaaaactg gaggccagca acggattacg cttttcaggc gcgaccagca tcaggccact
    38401 tgctgccgcc atctcaatca ccgccgtttt cccgtcgccg gtaaggccat aaaaagcatt
    38461 cactgtttcg cccatcggcc cgcagacttc aagcgtcacg atgttgccgc ccgtcgcggc
    38521 gaccatcgca tccaccgtcc cttcgccgcc atccgctatc ggcaaacaga tatagttcgc
    38581 atcgggaaag agggtcgaaa acccggcttt aattgcctga caacattttt ctgcacttaa
    38641 gctctcttta aaagagtctg gcgcaatgac aatcttcatt atcaatcctt tttcaacaaa
    38701 ttattcgatg cgttcccacg gaataagatt atccataaga acagccagta aaattccgac
    38761 cagtaagccg ttactgagca gaggacgcag cgttaatgga aggtcttgca gatacacagg
    38821 cggtaatgcc attaaaaata tgccgacaaa taacggcaat gcgagtcgat aaatattgcg
    38881 agcggtaaag gttatttgct ggctaaacac cagcgcggaa aagagtaaag gcagataaga
    38941 aaccagcatg accgcgctac tcacgggtaa agggatactg caaaacagtc gtgtgagtgc
    39001 cggaaccagc gccaccagca ggcaaataac gctgccataa ataaacgaac gccgcgtgta
    39061 atcgcctgtc tgggttaata aaccaatgga tgaaacaaac ggtgaaaatg gaattaccgc
    39121 cagcggtacg gttatcagcg tcataaatcc ggtcgccaca aagctgcgac gataacgcgt
    39181 attcccgcgc cctgctgcgg ataaaaaaca tccgtgcccc gaatcgcacc gtaggtattg
    39241 ctgatattca ccagacctgt aatcaccgcc gtcagaataa ttcccggaga caaagcacct
    39301 ccactgccga gcgggaacca ctgccagtgc aactcaccgg agagcgagtg cgaagaagga
    39361 aagcaaaagt accacaacaa ccagccggtt atggtgccga ccagcaggcc ataacgggca
    39421 aaacgttgcg gcaggaagat aatcatcccc agtaccaggc acatcaccgc caccgagagc
    39481 gcgaacggcg gtaactgaat tttaaaattt gggtcggcta tgccaaacgg cagcccgagc
    39541 atacctttga aaaagatagt ggtcagctgc gcgcccagca tcaacataaa caagaccatc
    39601 accgacggcg taaataaccg tgccaggcga tggcctaatc cgctaaaacc aatcaacatc
    39661 gtcagcacgc cggagagcgc aatccccacc gccaggctgg tagcgatatc gttgatcggt
    39721 gtcccgcgcg atgcttcgcc aagggtgata gtaaggatgg ttccccacca caggccaccc
    39781 ggcccttcca taatagcgcg acgatgaccg cagaacgcct gagcgaagca ggccagtgcg
    39841 gtagcaagaa aagcatattg cgtgagcgta agcaggctac tttgcggcaa ctgaaaagca
    39901 gaaagtagcg taggaggaac cacaaccgtg ttgcaaaaaa taaagaaaaa ccactgaaat
    39961 cctgataaca ggctttcgcg gctcactgca aaattgaaca taaacacacc ttaaccggag
    40021 aatgcccgcc gcaaggacgg gcattgcagg ggccagatta ctgctgatgt ttaaggataa
    40081 attgaccttt cggcgcaaca gggaagccct gttcaatgtc gtaaatcaca tcaccacgta
    40141 agatggtttt cgtgatacgc gcgccaatgg tacggccaac atacgggctg actttgtggc
    40201 gatattccag atcgtcattg gtaagaacat agctgctatt cggctgaatg aagacgaagt
    40261 cggcatcttt tcctggggcg atacggcctt tttgctgcag accgaaaata tctgctgcgt
    40321 tagtcgccat taatttgccg aacattggca gagacattcc gcgtttctgt accgcttcat
    40381 cgaacatcac gtccatacag ttttgcagac cggcaatacc gccccatgct tccatgatgt
    40441 tgccggcttt catttccgga gggcatggtg agtggtcgga aaccaggcag tcgatttcac
    40501 cgttaaacag tttttcccac atgcctttct ggttttccag atcgcggatc ggcggtgaac
    40561 acttcgccag agtgccgatt tcttcgaact gatcggtatc cagtacaaag taatgcgggc
    40621 aggattcaca agtaacgtcc tgaccttcct gacgtgcacg agtcacttct tcaacgcctt
    40681 ccgggctgct gatatggcaa acgtgcagac ggcaaccggc aactttcgcc agatacagca
    40741 cgcggcgaat cgcttccact tcggtaaata ccggacgcga agccacatag tcatgggcag
    40801 ttacgcgacc ttcacgtttc gcttcttcgc ccagtgcgtc acagatcagc gcgttttcgc
    40861 agtgcaccag caccggctgc cccagttcgc ccagcttctg cgcacctttg aagaactgcc
    40921 agtcgtttac atcacggaag tcgttgtcga taccgcgatc gccacaggtc gcaacgaagc
    40981 atttgaagcc gacaacgccc acttcatcca gctcatgcag acggtcgatg ttgtaagaca
    41041 ccaggccacc gagttgtgcc gcatcgatag tcagcttgcc tttagcggca tcgaacttca
    41101 gttcaattga agcgcggtca accgttgcag gcagctggtt gagcggcatt tcgatcatgg
    41161 tggtgatacc accttttgcc gctgcgcgag taccggtttc ataaccttcc cagtggctac
    41221 gacccggttc agaaatatgg gtgtgcgcat caaccatgcc cggcgaaacc accagaccag
    41281 acgcatccat aacttctttt gcatcgccca gatcctgacc gatagcagca attttcccgc
    41341 ctttaacggc gatatctaca acgcgagctt cgttttctaa aataacggtg ccgtttttaa
    41401 tgattaaatc aaaagacata acaaactcct tgtaaaatac agattggttt ttaactcaat
    41461 gttcaatatc gggattaatt aacctatggt tttttgctct cctgtttttt ctgctgttgt
    41521 acgtttcttt aataaggcgt aggccgcaaa ggcgacgatg acgccgacaa accatgaaac
    41581 acgcgataac ggttccataa agtgaataaa cttaccgcca agagataaaa taacggccac
    41641 cagagttact gaaaacgcag tgaggttaaa accgttatcg taatatttat aatcgccagg
    41701 tgcggtatac agttcatcaa gattaatttg tccgcgcatc accacaaaat aatgcgccat
    41761 catgacacca attaccggac caagcattcc gccgataata tcgaggaaaa gataaatgct
    41821 gtcctgattt tccattaatt tccacgggca gatcagcaag ctgataatac tggcaatcag
    41881 tacgccgttt ttataggtca gttttgtcgg tgcaatggcg gcaatctgat aaccggctgg
    41941 aataatatta ccggtcgcgt tagtggagat agttgtcatc agaataacca gtaccgcaaa
    42001 gaacgaggcg aacaggctgt cccaacgctg aacaatatcc agcacgttcc aggtatcagc
    42061 gccgtagtga atactggctc cggcaataat acataccccg gcgaccgcaa acagaatata
    42121 ggccacaact aaacccagcg tttgccccag cgcctgctca cgaaacgagt gggcgttttg
    42181 cgtaaagtcg gatgcgctca ccgccggtgc cgcccagacc gcaactaccg cgttaatcac
    42241 caccaggaac aggaagccac cgttttctgc tttctgaata ccgctcggaa tgtagtcaaa
    42301 gattggaccg atcccgacca gtgaaatcgc ccaaatcgcc ataccgccga aaacgatata
    42361 gatgcacggg ttaagaatgg cagtgaattt atttaaaact ttgccaccgc caaaacctat
    42421 accaacgttg accagccaga agattaagaa agtaattaag cccggtagag aaaggcctaa
    42481 cagagtgaaa tcaccaccga gagttaaaaa tcccggccag attttgccaa tcagaatcaa
    42541 gcaggccagt gaccccgcgt aacattgcaa accaaaccac atgatggcgg caattccgcc
    42601 ccttaataat ccgggaaaca gtgcaccacg tacaccgtaa gaagcacgca ggatcatggc
    42661 aaaaggcaca ccgtatttac tgcccgcagc accgtttaat accattaccg cggcaatgaa
    42721 aaaggcgctg aggataattg ccagcataat actaaaggta gacaagccga gaataaaaaa
    42781 gccgccgacc atcacataat tgggaacgtt atgaaccgaa cccatccata aggtaaaata
    42841 gttaaatgtt ttccaggtcc gctggctttg cgttttcggc aatagatctt cgctatagcc
    42901 gcgttgctgg agtagttttc tctgatgttc cataaatccc tcttaatttc acgtatcgaa
    42961 taacgttg
//
LOCUS       DAZFJF010000026        42151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFJF010000026 DAZFJF010000000
VERSION     DAZFJF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.026"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1379
                     /gene="espN"
                     /locus_tag="ACHKAU_001601"
     CDS             <1..1379
                     /gene="espN"
                     /locus_tag="ACHKAU_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFO5530691.1"
                     /translation="KEVSHMNGGQQFIWGFDKVIQEGLSGLIELSIDINDSTNHRDKS
                     SLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSILHSAIVKESSNICDYIQGAVRI
                     GMDFVPGTRSDLHSRSLQIKYEALKHIEKNINDNIIYHPSNNANFYSVIESIDGNDFI
                     YNEKQSKILEMKQDRGGNRYSAVDLNSSKYGYYEKVGGGFYRYIESFNPISSETPNKI
                     VYKGESVDLTKEPNSELYSGRYSINNKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDK
                     PYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIE
                     LSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYT
                     SDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKEN
                     ISMVNASK"
     gene            complement(1756..3117)
                     /gene="espK"
                     /locus_tag="ACHKAU_001602"
     CDS             complement(1756..3117)
                     /gene="espK"
                     /locus_tag="ACHKAU_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO5530692.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSL
                     SINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIV
                     AEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIVIQNTLTNAVWDTRTPRKYKQDSLIKRALNEHERGTKFKQCLKNH
                     NNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            3506..3646
                     /locus_tag="ACHKAU_001603"
                     /pseudo
     CDS             3506..3646
                     /locus_tag="ACHKAU_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(3728..3815)
                     /locus_tag="ACHKAU_001604"
     tRNA            complement(3728..3815)
                     /locus_tag="ACHKAU_001604"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3779..3781),aa:Ser,seq:tga)
     gene            4242..5360
                     /gene="hyaA"
                     /locus_tag="ACHKAU_001605"
     CDS             4242..5360
                     /gene="hyaA"
                     /locus_tag="ACHKAU_001605"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO5530693.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            5357..7150
                     /gene="hyaB"
                     /locus_tag="ACHKAU_001606"
     CDS             5357..7150
                     /gene="hyaB"
                     /locus_tag="ACHKAU_001606"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO5530694.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            7169..7876
                     /gene="hyaC"
                     /locus_tag="ACHKAU_001607"
     CDS             7169..7876
                     /gene="hyaC"
                     /locus_tag="ACHKAU_001607"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO5530695.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            7873..8460
                     /gene="hyaD"
                     /locus_tag="ACHKAU_001608"
     CDS             7873..8460
                     /gene="hyaD"
                     /locus_tag="ACHKAU_001608"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO5530696.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            8457..8855
                     /gene="hyaE"
                     /locus_tag="ACHKAU_001609"
     CDS             8457..8855
                     /gene="hyaE"
                     /locus_tag="ACHKAU_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO5530697.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            8852..9709
                     /gene="hyaF"
                     /locus_tag="ACHKAU_001610"
     CDS             8852..9709
                     /gene="hyaF"
                     /locus_tag="ACHKAU_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO5530698.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            9843..11388
                     /gene="appC"
                     /locus_tag="ACHKAU_001611"
                     /pseudo
     CDS             9843..11388
                     /gene="appC"
                     /locus_tag="ACHKAU_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            11400..12536
                     /gene="appB"
                     /locus_tag="ACHKAU_001612"
     CDS             11400..12536
                     /gene="appB"
                     /locus_tag="ACHKAU_001612"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5530699.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            12549..12641
                     /gene="cbdX"
                     /locus_tag="ACHKAU_001613"
     CDS             12549..12641
                     /gene="cbdX"
                     /locus_tag="ACHKAU_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO5530700.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            12721..14019
                     /gene="appA"
                     /locus_tag="ACHKAU_001614"
     CDS             12721..14019
                     /gene="appA"
                     /locus_tag="ACHKAU_001614"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO5530701.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(14134..16314)
                     /gene="etk"
                     /locus_tag="ACHKAU_001615"
     CDS             complement(14134..16314)
                     /gene="etk"
                     /locus_tag="ACHKAU_001615"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO5530702.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(16334..16780)
                     /gene="etp"
                     /locus_tag="ACHKAU_001616"
                     /gene_synonym="yccY"
     CDS             complement(16334..16780)
                     /gene="etp"
                     /locus_tag="ACHKAU_001616"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO5530703.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(16768..17907)
                     /gene="gfcE"
                     /locus_tag="ACHKAU_001617"
     CDS             complement(16768..17907)
                     /gene="gfcE"
                     /locus_tag="ACHKAU_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO5530704.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(17953..20049)
                     /gene="gfcD"
                     /locus_tag="ACHKAU_001618"
     CDS             complement(17953..20049)
                     /gene="gfcD"
                     /locus_tag="ACHKAU_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5530705.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(20049..20795)
                     /gene="gfcC"
                     /locus_tag="ACHKAU_001619"
     CDS             complement(20049..20795)
                     /gene="gfcC"
                     /locus_tag="ACHKAU_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5530706.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(20792..21436)
                     /gene="gfcB"
                     /locus_tag="ACHKAU_001620"
     CDS             complement(20792..21436)
                     /gene="gfcB"
                     /locus_tag="ACHKAU_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO5530707.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(21543..21848)
                     /gene="gfcA"
                     /locus_tag="ACHKAU_001621"
     CDS             complement(21543..21848)
                     /gene="gfcA"
                     /locus_tag="ACHKAU_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO5530708.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(22290..22502)
                     /gene="cspH"
                     /locus_tag="ACHKAU_001622"
     CDS             complement(22290..22502)
                     /gene="cspH"
                     /locus_tag="ACHKAU_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO5530709.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            22788..23000
                     /gene="cspG"
                     /locus_tag="ACHKAU_001623"
     CDS             22788..23000
                     /gene="cspG"
                     /locus_tag="ACHKAU_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO5530710.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            23011..23199
                     /gene="ymcF"
                     /locus_tag="ACHKAU_001624"
     CDS             23011..23199
                     /gene="ymcF"
                     /locus_tag="ACHKAU_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5530711.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            23174..23404
                     /gene="ymcE"
                     /locus_tag="ACHKAU_001625"
     CDS             23174..23404
                     /gene="ymcE"
                     /locus_tag="ACHKAU_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO5530712.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            23394..23567
                     /gene="gnsA"
                     /locus_tag="ACHKAU_001626"
     CDS             23394..23567
                     /gene="gnsA"
                     /locus_tag="ACHKAU_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5530713.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(23615..24688)
                     /gene="yccM"
                     /locus_tag="ACHKAU_001627"
     CDS             complement(23615..24688)
                     /gene="yccM"
                     /locus_tag="ACHKAU_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5530714.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(24760..27504)
                     /gene="torS"
                     /locus_tag="ACHKAU_001628"
     CDS             complement(24760..27504)
                     /gene="torS"
                     /locus_tag="ACHKAU_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO5530715.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            27587..28615
                     /gene="torT"
                     /locus_tag="ACHKAU_001629"
     CDS             27587..28615
                     /gene="torT"
                     /locus_tag="ACHKAU_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO5530716.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(28588..29280)
                     /gene="torR"
                     /locus_tag="ACHKAU_001630"
     CDS             complement(28588..29280)
                     /gene="torR"
                     /locus_tag="ACHKAU_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO5530717.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            29410..30582
                     /gene="torC"
                     /locus_tag="ACHKAU_001631"
     CDS             29410..30582
                     /gene="torC"
                     /locus_tag="ACHKAU_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO5530718.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            30582..33128
                     /gene="torA"
                     /locus_tag="ACHKAU_001632"
     CDS             30582..33128
                     /gene="torA"
                     /locus_tag="ACHKAU_001632"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO5530719.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            33125..33724
                     /gene="torD"
                     /locus_tag="ACHKAU_001633"
     CDS             33125..33724
                     /gene="torD"
                     /locus_tag="ACHKAU_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO5530720.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(33817..34122)
                     /gene="cbpM"
                     /locus_tag="ACHKAU_001634"
     CDS             complement(33817..34122)
                     /gene="cbpM"
                     /locus_tag="ACHKAU_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO5530721.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(34122..35042)
                     /gene="cbpA"
                     /locus_tag="ACHKAU_001635"
     CDS             complement(34122..35042)
                     /gene="cbpA"
                     /locus_tag="ACHKAU_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO5530722.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            35302..36561
                     /locus_tag="ACHKAU_001636"
     CDS             35302..36561
                     /locus_tag="ACHKAU_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFO5530723.1"
                     /translation="MSSNIHGISCTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQS
                     DNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEQKV
                     NEVKNFLTALSDSICSLFIVNGNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPP
                     ELFAQAYELNNKERNAQIDFLTRYAIY"
     gene            36853..38094
                     /gene="agp"
                     /locus_tag="ACHKAU_001637"
     CDS             36853..38094
                     /gene="agp"
                     /locus_tag="ACHKAU_001637"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO5530724.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(38132..38359)
                     /gene="yccJ"
                     /locus_tag="ACHKAU_001638"
     CDS             complement(38132..38359)
                     /gene="yccJ"
                     /locus_tag="ACHKAU_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO5530725.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(38380..38958)
                     /gene="wrbA"
                     /locus_tag="ACHKAU_001639"
     CDS             complement(38380..38958)
                     /gene="wrbA"
                     /locus_tag="ACHKAU_001639"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5530726.1"
                     /translation="MINHILSHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            complement(38955..40265)
                     /locus_tag="ACHKAU_001640"
     CDS             complement(38955..40265)
                     /locus_tag="ACHKAU_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5530727.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYADRFPDSPNLKLFGLVKKDITVGELAQKWLTLKVMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSRKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLSRQHQITVQLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            complement(40318..40602)
                     /locus_tag="ACHKAU_001641"
     CDS             complement(40318..40602)
                     /locus_tag="ACHKAU_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5530728.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            complement(40648..40899)
                     /locus_tag="ACHKAU_001642"
     CDS             complement(40648..40899)
                     /locus_tag="ACHKAU_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5530729.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            complement(40887..41120)
                     /locus_tag="ACHKAU_001643"
     CDS             complement(40887..41120)
                     /locus_tag="ACHKAU_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530730.1"
                     /translation="MKITFESYGLTASVVISSSIFESRKHQHIVDAVKLKAPEVTVTT
                     HGLFRIRTTLTTTHLSACRVYDMALKEYNQCSS"
     gene            complement(41264..41644)
                     /locus_tag="ACHKAU_001644"
     CDS             complement(41264..41644)
                     /locus_tag="ACHKAU_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5530731.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNDIWQAVAREVDKKPNIASVQPVQPVQPVQPVQHNIIGDLLRKSRKEARRAGRKQK
                     RWEGACKALQELNKYRDLINELSE"
     gene            complement(41680..41835)
                     /locus_tag="ACHKAU_001645"
     CDS             complement(41680..41835)
                     /locus_tag="ACHKAU_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5530732.1"
                     /translation="MANLQLARSGIRFVPIPITADAELHLFGEILSRKLDELEKLVEE
                     ADTSPTV"
     gene            complement(41828..>42151)
                     /locus_tag="ACHKAU_001646"
     CDS             complement(41828..>42151)
                     /locus_tag="ACHKAU_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5530733.1"
                     /translation="HGVIVQAAWFVKASLEQNALSGNSTVTPDGWISCSERMPEKNQN
                     VLISVNFDRSLVEPLICSARYTGSTFRRGQITVAPGNGIDGNGIEQATHWMPLPEPPQ
                     EVNRG"
BASE COUNT        10267 a        10274 c        10722 g        10888 t
ORIGIN      
        1 aaaaagaagt atcccatatg aatggcgggc agcagtttat ttgggggttt gataaggtta
       61 ttcaagaagg cttaagtgga ttgattgagt taagtatcga tattaatgat agtacaaatc
      121 atcgtgataa gtcttctctt tctcctgatg ggagagctgt gttacatttt ttaggtacaa
      181 tttggaatat ggcgatggga gctgtacctg gttataatgc attgtctggt gtaagtagca
      241 ttttacatag tgctatagta aaagaatcta gcaatatctg tgattatatt cagggggctg
      301 tacgtattgg aatggacttt gttccaggca ctcgctctga cttacatagc cgttcgctgc
      361 agataaaata tgaggctttg aagcatatag aaaaaaacat taatgataat attatttatc
      421 atccgagtaa taatgctaat ttctattctg taattgagtc aattgatggt aatgacttta
      481 tatataacga aaaacaatct aaaatattag aaatgaaaca ggatcgtggg gggaatagat
      541 atagtgcagt agatcttaac tcttctaagt atgggtatta tgagaaagtt ggcggtggtt
      601 tttatagata tatagaatcc tttaacccca tatcttcaga gacaccaaat aaaatagtct
      661 acaaggggga atcagtagat ttaactaagg agccaaattc ggaattatat tcaggtaggt
      721 attctataaa taacaaacag gttaatgttt atttctttcg tgacgctgat ggtacatttt
      781 ataaatcaga aggtcttcat ggtgggggag ttattagata catagataaa ccgtattctc
      841 agttaagaga aggagatatt gggtatgatg aggatttgtt ggatatatac gatgattctc
      901 cggtgcttga agacacgttg cctgctttat cttctgaaat agtaccaact ccagaacata
      961 gtattaaaca aatttattcg aaaattaagg aggggcacat agaactgtcc gattcagaca
     1021 tcatattgtg tcgcggcaca accggtattc aagctgaaaa tatcgttgaa tataaaactg
     1081 ctggagggct tcctgattca aatccaaatg taaaagcacc agatgaatat atggcacaac
     1141 agcaggtacg tattggaaga atattgcctg aatacacatc ggatcttagc gttgctgatc
     1201 ggtttagtcg tgagcattat ctaatagttg ttaaagtaaa ggcaaaatat atcacacgag
     1261 gaagtgttac agagagtggt tgggttatag ataagaccgc acctgttgaa ccacttgcga
     1321 taattgatag aacttttggt atgaaggaaa atatctcaat ggtaaatgca tcgaaatagt
     1381 ttttttacaa tctatgtcct gcctcctctg gtaaaaacga tgctacatct ttgagatgtt
     1441 gcacggcagt acggtgttga ccgatatata gtgaagtaca catcggtcaa cgaattacca
     1501 ttgtcagcaa tatcatcctg ataaactcag tactcgtgag ccgcttgatg acgggcggaa
     1561 tagcccaaaa ggtaaacgat ccgcatggca gtccgtcgga aagctgctgc ttcatacgac
     1621 cgcttaaacc gtcagttagt gtcagtatcg ctgaagatca gcttcttttg ctgatttact
     1681 ctgtttttac ctgccctgat gaaatactct ttaatcataa cattgattat atcgaaaata
     1741 tattttttgc tatcattaag aatatttata tgtggaaccg gagtattttg gacggtagtc
     1801 tttccactct ggtgtatcta atattctttc tgctggtatc tctttgccat gcagataaaa
     1861 ttttatgttt ttcattattt ggctgttgtc tttattacga taaatccatc gtaattcgct
     1921 ggcggtaata ctctgaccat agccctctga cttgttagct atgagtttga gtgaattgac
     1981 aagcccatcc gcacaaaaca taaccgttct gttgcgaatt aatgtttgaa actctaggcc
     2041 tgccttgctt gtttttgccc ataacttgtc tcgattgtat atggaaaggt cggccatgat
     2101 gacattataa ttattgtgat tttttaagca ctgtttaaat tttgttcctc tttcatgttc
     2161 atttaatgct cttttgataa gcgagtcttg cttatattta cggggggttc tggtgtccca
     2221 tactgcgttt gttaatgtat tctgaattac gatatctttt ttggaaaagt cgttaaccag
     2281 tttgatgctg ttgacaagtt tttctctggg agaggatagc ccatagacaa tgtctcccgg
     2341 acgaaaatgc aaggcagcat ccagtgcgtt gccctgaaaa gtgatgtttt tttctttaat
     2401 tacattcgga gagagtagta cattttgtaa atcaagatcc agtccatcag gaaccccttc
     2461 agctacgatg ttccatgtag ttttctgctc cacatgaatg gttacagatc tcagactctc
     2521 cgggagtttc tccggcagta taatattgct acatccagtc agagatagcg tctgtagtga
     2581 cggtgatatc aggctgtcaa ttctggcctg attttctggg gtgtagcgat tgatgctgag
     2641 agataataac ccgtttggaa cattttttat gccgtctgtg gcgtaacctc tgatctcaag
     2701 atgacggaca ctctctggca ctccagatat atggcagtgg caaaccgtca ggcattcgat
     2761 gccttcagat atgatgtgta taaatggtga atcagttaat tcaatatgta gattttttac
     2821 tgtcgatggt atgtctgtga tttgtaattt ttcacagtta atgacggaca ggttttgtgt
     2881 attaaccggt aatcctgtaa gggtggtcag gttattgcaa ttttcgattg taactgatgt
     2941 tatttcgttt ggtaactgtg gcagatactc aatatcacaa tctttgatat ataaacgacc
     3001 aatggcccgc gcttcttcta tctgagttgg tgtgatttcg cttttgatgc ctgctgatgt
     3061 ttctgcggaa ttggagtaga atgtcccgga cggtcgtaat tgcgatgtag gaagcattga
     3121 taaattccat ttttagaagt aaataacagt gtaagttcta tccatactgc taatcaacaa
     3181 gctaatttta gcaaccagca aagccaaggt gtcaatatta gaaaagagag ttatatctca
     3241 aaatcaacag actgttgatt ttaacttttg cgaatacttt ctccagtccg tcatgcatgg
     3301 attaggattg ctcattttta taccatttgt tgttttttat agcgatttgc taaaagggag
     3361 aagaaaaaac gttctccaaa attagattgc aactgtttga ttttatggat agaggcatgc
     3421 tgtttactag acagtaaaaa gcatgccaaa tttgctatta accttttgaa tataaagcac
     3481 taattatcta tttctgttgc cttcttcagt agatctggag atcgcaacgg aggaagaaaa
     3541 ctcgttgctg gaagcctgga agaagtatcg ggtgttgctg aaccgtgttg atacatcaac
     3601 tgcacctgat attgagtggc cggaagaacc aagatcatcc gaataaaaat ataccaattg
     3661 gtataccaag tttataccaa gagccagaaa agcaaagggg ctaccatttg gtaacccctt
     3721 gattatttgg cggaagcgca gagattcgaa ctctggaacc ctttcgggtc gccggttttc
     3781 aagaccggtg ccttcaaccg ctcggccaca cttccggaat gacgcgcact ataaacatcc
     3841 cgatgcggcg tgtaaacccc taatttgttt gtttgcctga aaaacagcca aaagtgcatt
     3901 gatagcgtga aataacagca gattgatcat ttcatcacca tgaattcctt ctcttttact
     3961 cgtttagcaa ccggctaaac atccccaccg cccggccaaa agaaaaatag gtccattttt
     4021 atcgctaaaa gataaatcca cacagtttgt attgttttgt gcaaaagttt cactacgctt
     4081 tattaacaat actttctggc gacgtgtgcc agtgcagaag gatgagcttt cgttttcagc
     4141 atctcacgtg aagcgatggt ttgccttgct acagggacgt cgcttgccga ccataagcgc
     4201 ccggtgtcct gccggtgtcg caaggagaag agacgtgcga tatgaataac gaggaaacat
     4261 tttaccaggc catgcggcgt cagggcgtta cccggcgcag ctttctcaaa tattgtagtc
     4321 tggctgccac gtcgctggga ttaggcgcgg gaatggcacc aaagattgcc tgggcgctgg
     4381 agaacaaacc ccgcattccg gtggtatgga tccacggtct ggaatgcacc tgctgtaccg
     4441 aatcttttat ccgctccgct cacccactgg cgaaggacgt catcctttcc ctgatttccc
     4501 tcgattatga cgatactttg atggctgccg ccggaaccca ggcggaagaa gtcttcgaag
     4561 acatcatcac gcaatacaat ggcaaatata tcctcgcagt agaaggtaat ccgccgctgg
     4621 gcgagcaggg gatgttctgt atcagcagcg gtcgaccgtt tattgagaaa ctcaaacgtg
     4681 ccgctgccgg agccagcgcg attatcgcct ggggaacctg cgcgtcctgg ggctgcgtgc
     4741 aggccgcgcg gcccaatccg acgcaggcaa cgcctatcga caaagtcatc accgacaaac
     4801 ccattatcaa agtacctggc tgcccgccga tcccggatgt gatgagcgcc atcattactt
     4861 acatggtgac ctttgatcgc ttgccagatg tcgacagaat gggccgtccg ctgatgttct
     4921 atggtcagcg aatccacgat aaatgctatc gccgcgccca cttcgacgcc ggagagttcg
     4981 tccagagttg ggatgatgac gctgcccgca aaggttactg cctgtacaaa atgggctgca
     5041 aagggcctac cacctataac gcctgttctt ccacacgctg gaatgatggc gtttctttcc
     5101 caatccagtc tggtcacggc tgcctgggct gtgcggaaaa tggtttctgg gatcgcggtt
     5161 cgttctacag ccgcgtggtc gatattccgc aaatgggtac tcattccacc gccgataccg
     5221 tcggtttaac cgcgcttggc gtggtggcag cggctgttgg tgtgcacgca gtcgccagcg
     5281 ccgttgacca gcgcagacgt cataaccagc aacctacaga aaccgaacat cagccaggca
     5341 atgaggataa acaggcatga gcactcagta cgaaactcag ggatacacca tcaataatgc
     5401 cggacgccgc ctggtggtcg acccgattac gcgcatcgaa ggccacatgc gctgcgaagt
     5461 gaatattaac gatcagaatg tgatcaccaa tgccgtctcc tgcggcacca tgtttcgcgg
     5521 gctggagatc atcctacaag ggcgcgaccc gcgcgatgcg tgggcgttcg ttgaacgtat
     5581 ctgcggcgtc tgtactggcg tacacgccct ggcttcggtt tacgccatcg aagatgctat
     5641 cggtattaaa gtgccggaca acgcaaatat catccgcaac attatgctgg caacgctctg
     5701 gtgccacgat catctggtgc acttctatca gcttgccggg atggactgga tcgatgtgtt
     5761 agatgcgctg aaagccgacc cgcggaaaac ctccgaactg gcgcaaagtc tctcctcttg
     5821 gccgaaatca tcccctggct atttcttcga cgtacaaaac cgcctgaaaa aatttgttga
     5881 aggcgggcag ttggggatct tccgcaatgg ctactggggg cacccgcagt acaaactgcc
     5941 gccagaagct aacctgatgg gctttgccca ctatctcgaa gctctcgatt tccagcgtga
     6001 aattgtcaaa atccacgcgg tctttggcgg taaaaacccg catccaaact ggattgtcgg
     6061 cgggatgcct tgcgccatca acattgacga aagcggcgcg gtcggggcag tcaatatgga
     6121 acgcctgaac ctggtgcagt caattatcac ccgcacggcg gacttcatta acaacgtgat
     6181 gatccccgac gccttagcca tcggtcagtt caacaaaccg tggagcgaaa tcggcactgg
     6241 tctttctgat aaatgcgttc tcagctacgg cgcattcccg gatattgcca acgactttgg
     6301 cgagaaaagt ctgctgatgc ctggcggcgc ggtgattaac ggcgacttca acaatgtgct
     6361 gccagtggat ttggttgatc cgcagcaggt gcaggagttt gtcgaccacg cctggtatcg
     6421 atatcccaac gatcaggtcg ggcgtcatcc gttcgatggc atcaccgacc cgtggtacaa
     6481 ccccggcgat gtcaaaggca gcgataccaa cattcagcag ctgaatgaac aggaacgcta
     6541 ctcgtggatc aaagcgccac gctggcgcgg taacgcgatg gaagtggggc cgctggcgcg
     6601 cacgttaatc gcttatcaca aaggcgatgc tgcgaccgtt gagtcggtcg atcgcatgat
     6661 gtcggcgttg aacctgccgc tttccggtat ccagtcaacg ttaggccgca ttttgtgccg
     6721 cgcgcacgaa gcgcagtggg ccgcaggtaa gttgcagtat ttcttcgaca agctgatgac
     6781 caacctgaaa aacggcaatc tcgccactgc ttccacggaa aaatgggaac ctgcaacctg
     6841 gccgacagag tgccgtggtg tcggttttac cgaagcgccg cgcggggcgt taggccactg
     6901 ggccgccatt cgcgatggca agattgatct ctaccagtgc gtggtgccga ccacctggaa
     6961 cgccagcccg cgcgatccca aagggcagat tggcgcttat gaagcggcgc tgatgaacac
     7021 caaaatggcg atccccgagc aaccgctgga gatcctgcgt actctgcaca gctttgaccc
     7081 gtgcctcgcc tgttcaacac acgtgctggg cgacgacggt agcgagctga tctctgtgca
     7141 ggtgcgttaa cagcgaagga gaatcatcat gcaacagaaa agcgacaacg ttgtcagcca
     7201 ctatgtcttt gaagcgccag tccgcatctg gcactggttg acggtgttat gcatggcggt
     7261 gttgatggtc accgggtact ttatcggcaa gccgctacct tccgtcagcg gcgaggcgac
     7321 gtatctgttc tatatgggct acatcaggtt aattcacttc agcgccggga tggtttttac
     7381 cgtggttttg ctgatgcgga tctactgggc ttttgttggc aatcgatact cccgcgagct
     7441 gtttatcgtg ccggtatggc gtaaaagctg gtggcagggc gtgtggtatg aaatccgctg
     7501 gtatctgttt ctggcaaaac gtccgagtgc cgatataggc cataatccca tcgcccaggc
     7561 ggcgatgttc ggctatttcc tgatgtcggt ctttatgatc atcactggtt ttgcgctgta
     7621 tagcgaacac agccagtacg ctatttttgc gccgttccgt tatgtggtgg aatttttcta
     7681 ctggatgggt ggcaactcaa tggacattca cagctggcat cggctgggga tgtggctgat
     7741 tggcgcgttt gtgatcggtc atgtctacat ggcgctgcgt gaagacatca tgtccgacga
     7801 cacggtgatc tccaccatgg tcaacggcta ccgtagccac aaatttggca aaataagtaa
     7861 caaggagcgt tcatgagcga gcaacgcgtg gtggtcatgg ggctgggcaa cctgctgtgg
     7921 gccgatgaag gcttcggcgt gcgggtggcg gaacggctgt atgcccatta ccactggccc
     7981 gagtatgtgg agattgtcga tggcggtact cagggactga acttgctggg gtatgtcgaa
     8041 agcgccagcc atctgttgat tctcgatgcc attgactacg ggctggaacc tggaacgctg
     8101 cgaacctatg ccggagaacg cattccggct tatctcagcg cgaagaaaat gagcctgcat
     8161 cagaacagtt tctccgaagt gttggcgctg gcggatatcc gcggacatct gccagcacat
     8221 attgccctcg tcggtctgca acccgcaatg ctcgacgact acggcggtag cctgagcgaa
     8281 ctggcacggg agcaactgcc cgctgcggaa caggcggcgc tggcgcagct ggctgcgtgg
     8341 ggaattgtgc cgcaaccggc taatgaatcg cgctgtctca attatgactg tctgtcgatg
     8401 gaaaattacg aaggcgttcg cttgcgccag taccggatga cacaggagga gcagggatga
     8461 gcaacgacac gccatttgat gcgttgtggc aacgaatgct ggcgcgcggc tggacgccag
     8521 tcagtgaatc ccgtcttgac gactggctta cgcaagcgcc agacggcgtg ctgttattaa
     8581 gcagtgaccc gaaacgcacg ccagaggtca gcgataatcc ggtaatgatt ggcgaattac
     8641 tgcgcgagtt tcccgactat acatggcagg tggcgattgc tgaccttgag cagagcgaag
     8701 ccatcggcga tcgctttggc gcctttcgct ttcctgccac tttagtgttt accggcggaa
     8761 actatcgcgg cgtgctgaat ggtattcacc cgtgggcgga actgataaac ctgatgcgcg
     8821 ggcttgtcga accgcagcag gagcgtgcct catgagcgaa acatttttcc atctgctggg
     8881 gccaggaacg caaccgaacg atgacagttt cagcatgaat ccactgccga tcacctgtca
     8941 ggtgaatgat gaaccgagta tggcggccct ggagcaatgt gctcacagcc cgcaggtgat
     9001 tgcgctgtta aacgagttac aacatcaact aagcgaacgc caaccgccgt tgggcgaggt
     9061 gctggcagtc gatctgttaa atctcaacgc cgacgatcgt cactttatca atacgcttct
     9121 cggggaaggg gaagtgtcag tgcgcattca gcaggctgac gacagtgaaa gtgaaataca
     9181 ggaggcgatc ttctgcggat tatggcgggt gcgcagacgt cgcggcgaaa agttgctgga
     9241 ggacaaactg gaggctggct gcgcgccgct ggcgttgtgg caggcggcaa cgcaaaatct
     9301 cttgccgaca gattcgctgt taccgccgcc cattgatggc ctgatgaatg gcctaccgtt
     9361 ggcgcatgag ttactggcac atgtacgtaa ccccgacgcg cagccgcaca gcattaatct
     9421 gacgcaatta cccatcagcg aggctgatcg gctttttctc tcacgtctct gtgggccggg
     9481 aaatattcag attcgtacca ttggctatgg cgagagctat atcaacgcca cggggttacg
     9541 ccatgtctgg catttacgct gtacggacac cttaaaaggc ccgttactgg aaagttatga
     9601 aatctgccca ataccggaag tggtgctggc agcgccagaa gatttggtcg actctgcgca
     9661 gcggcttagc gaggtatgtc agtggctggc ggaagctgca ccgacgtaaa aagacggtaa
     9721 gtatcgcttt cagtcttatg aatatcgcaa tcggcgaata cctctggtcg tagagtttca
     9781 ggataaagag ggagatctac cattatcggg ttatttttct ctcttcgcct acaggagtgc
     9841 gcatgtggga tgtcattgat ttatcgcgct ggcagtttgc tctgacctgc gctgtatcac
     9901 tttttatttg taccccttac cctggggctg atttttttgc tggctatcat ggaaaccatt
     9961 tacgtggtca ccggcaaaac aatctaccgc gatatgacgc gcttctgggg taagctcttc
    10021 ggtatcaatt ttgctcttgg cgtggctacc ggcctgacca tggagtttca gtttggtact
    10081 aactggtcat tctattccaa ctatgtgggc gatatttttg gcgcaccact ggcgatggaa
    10141 gcattaatgg ccttcttcct cgaatccacc tttgtcgggc tgttcttctt cggctggcaa
    10201 cggctgaata aataccagca cctgctggtg acgtggctgg tggcgttcgg ttcaaatctc
    10261 tctgcgttgt ggatattgaa tgccaacggt tggatgcaat acccgaccgg tgcgcatttt
    10321 gatatcgaca ccctgcgtat ggagatgacc agcttcagcg agctggtctt taacccggtc
    10381 agccaggtga aatttgtgca caccgtaatg gcgggctacg tgaccggggc catgtttatt
    10441 atggcgatca gcgcctggta tttactgcgc ggacgggagc gcgatgtcgc attacgttcg
    10501 tttgccatcg gttccgtctt cggtactctg gcgattatcg gtaccctgca actcggagac
    10561 agttctgcgt atgaagtcgc gcaagtacaa ccggtaaaac tggcggcgat ggaaggggag
    10621 tggcaaacgg aacctgcacc tgcaccgttc catgtggttg cctggccgga acaggatcaa
    10681 gagcgtaacg cctttgccat caaaattccc gcgctgctag ggatcctcgc cactcactca
    10741 ttagataaac ccgtgccggg tctgaagaat ttgatggctg aaacctaccc acgcttgcaa
    10801 cgcggacgta tggcctggct gttaatgcag gaaatatcgc aaggcaatcg tgagccgcat
    10861 gtgttgcagg cattccggga actggaaggt gacctgggct acggcatgtt gctctcccgc
    10921 tatgcgccgg atatgaatca tgtcacagcc gcacagtacc aggcggcgat gcgtggcgcg
    10981 atacctcagg ttgcgccggt attctggagt ttccgcatca tggtgggctg tggttccctg
    11041 ctgctactgg tgatgctgat tgcgcttgtc cagacgctgc gtggcaaaat cgaccagcat
    11101 cgctgggtgc tgaaaatggc gctctggagt ttgccgttgc cgtggattgc gattgaagcc
    11161 gggtggttta tgaccgagtt tggtcgtcag ccgtgggcga tacaggacat cttaccgaca
    11221 tactccgcgc actccgcttt aaccacagga caactggctt tctcactgat catgatcgta
    11281 gggctttaca ccctgttctt aatcgccgaa gtctacctga tgcagaaata tgcccgtctg
    11341 gggccgagcg cgatgcagag tgaacaaccg acgcagcaac aggggtaaag gagaaaatca
    11401 tgtttgatta tgaaacattg cgcttcatct ggtggctgct gattggcgtg atcctggtgg
    11461 tctttatgat ctccgacgga tttgacatgg ggatcggctg tctgctgccg ctggtggcgc
    11521 gtaatgatga tgaacgccgg atagtgataa acagcgttgg tgcacactgg gaaggcaacc
    11581 aggtctggtt gatcctcgct ggtggggcat tatttgccgc ctggcccaga gtgtatgcag
    11641 cggcgttttc cggcttttat gtggcgatga tcctggtgct gtgctcactg ttcttccgcc
    11701 cgctggcctt tgattatcgc ggaaaaatcg ccgatgcacg ctggcgtaaa atgtgggacg
    11761 ccggtctggt catcggcagt ctggtgccgc cggtagtctt cggtatcgcc ttcggcaact
    11821 tgttgctcgg cgtgccgttt gccttcacac cgcaattacg cgtggagtat ctcggcagct
    11881 tctggcaact gctgacgcca ttccctttat tgtgcggatt gctcagcctt gggatggtga
    11941 ttttgcaagg tggcgtctgg ttacaactga aaactgttgg tgtgattcat ctgcgttcac
    12001 agctggccac caaacgcgct gcactgttgg tgatgctgtg ctttttgctg gcgggttact
    12061 ggctgtgggt cggtattgat ggctttgtac tgctcgccca ggatgctaac ggtccttcca
    12121 atccgttaat gaaactggtg gcagtgctac ctagtgcctg gatgaataat ttcgtcgaat
    12181 cgcccgtttt gtggatcttc ccgctgctgg ggttcttctg cccattgctg acggtgatgg
    12241 cgatttatcg tggtcgcccg ggttggggat ttttaatggc gtcattgatg caatttggcg
    12301 tgattttcac ggcaggcatc acgctgttcc cctttgtcat gccgtcaagc gtgagtccaa
    12361 tctccagcct gacgttgtgg gacagcactt ccagtcagct gacgctgagc attatgttgg
    12421 taatcgtgct gatatttttg cccattgtgt tgctctacac tctctggagc tactacaaaa
    12481 tgtggggacg catgacaaca gaaactctcc gccgtaacga aaacgagttg tactaaggag
    12541 caggaacaat gtggtattta ctttggttcg tcggcatttt gttgatgtgt tcgctctcca
    12601 cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag gcctgatgcg
    12661 gcgcattagc atcgcatcag gcaatcaata atgtcagata tgaaaagcgg aaacatatcg
    12721 atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc gcaatctgca
    12781 ttcgctcaga gtgagccgga gctgaagctg gaaagtgtgg tgattgtcag tcgtcatggt
    12841 gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga cgcatggcca
    12901 acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat cgcctatctc
    12961 ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa gggctgcccg
    13021 cagtctggtc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa aacaggcgaa
    13081 gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca ggcagatacg
    13141 tccagtcccg atccgttatt taatcctcta aaaactggcg tttgccaact ggataatgcg
    13201 aacgtgactg acgcgatcct cagcagggca ggagggtcaa ttgctgactt taccgggcat
    13261 cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt ttccgcaatc aaacttgtgc
    13321 cttaaacgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc atcggaactc
    13381 aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc aatgctgacg
    13441 gagatatttc tcctgcaaca agcacaggga atgccggagc cggggtgggg aaggatcacc
    13501 gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta tttgctacaa
    13561 cgcacgccag aggttgcccg cagccgcgcc accccgttat tagatttgat caagacagcg
    13621 ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac ttcagtgctg
    13681 tttatcgccg gacacgatac taatctggca aatctcggcg gcgcactgga gctcaactgg
    13741 acgcttcccg gtcagccgga taacacgccg ccaggtggtg aactggtgtt tgaacgctgg
    13801 cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca gactttacag
    13861 cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt gaaactgacc
    13921 ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg ttttacgcaa
    13981 atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa gagcattcag
    14041 ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt agaccggaaa
    14101 aggcgttcac gccgcatccg gccactttca gttttactct ttctcggagt aactataacc
    14161 gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac cattgaggat
    14221 agcgccttta atattgacgc ctgcctgttc cagacgctgc attgacaaac tcacctcttt
    14281 ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccaacagaac gccccacgac
    14341 cgcggcatca ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat cgtaatggtc
    14401 gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag acgggttagg
    14461 tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct ggatcacttt
    14521 gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac tcacggtaaa
    14581 caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt tttgatcgga
    14641 ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt ctggcgtcgc
    14701 accggtgatc atcagaatgt tattctccgt ctccatcata gcgaaatgca gactggttcg
    14761 tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca ggaaggggat
    14821 atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca gacgggtgcg
    14881 tttatccagc cactcggaca ttgggatagt ggcataaacg ctgatgccgt gctcttccag
    14941 ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg cacccacaga
    15001 aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg gtttcactgg
    15061 ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac tggatttcga
    15121 aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc ccgcttctac
    15181 gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata cccgcttatt
    15241 caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac gataagttgg
    15301 gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct cattgagttg
    15361 attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa ggtcaaccga
    15421 atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct cgctgcgcac
    15481 ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg cctggcgagc
    15541 gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa tcaactgggg
    15601 atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt cgctaacggt
    15661 aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca ggacaaactg
    15721 tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt tttccagacg
    15781 ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt ggccgttttc
    15841 cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat gcatccagct
    15901 gatcgccagc tcacctggtt tttctttggt taatctcgcc cagccgcgac ccacaatcgg
    15961 aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt taccgagaat
    16021 catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt tagggatcat
    16081 atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga ccagagtatc
    16141 tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg tgaataacgc
    16201 ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac gaagcagatc
    16261 gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg tcatcgtatt
    16321 ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg cgctccaaca
    16381 taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg atctctttct
    16441 gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca attgcggtaa
    16501 cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt gccatctcag
    16561 cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg gcaacatctg
    16621 ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat ttgactttta
    16681 cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa atgttgccgg
    16741 tacaaaccac caggattgag ttaaatttta gttgggccat gtttgaatcc accgtgaagt
    16801 ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca cacggttcca
    16861 acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct ggaattccgt
    16921 tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg cgattttgcc
    16981 attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag cgacatcctg
    17041 attcataccc tcagcgttac ccagcgcttc cgccagggtc ataccgctgc ggtccatttt
    17101 cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat cgttgcgcgg
    17161 aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc cacgctgcat
    17221 cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca cgttgcgcca
    17281 gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg tcagaggaat
    17341 attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg ctttttgcga
    17401 gcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat ccagacgcgc
    17461 tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc gaccaatata
    17521 ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg cgctacggta
    17581 ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca acacatcccc
    17641 aatgccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt tagcctgcga
    17701 cataatcggt ttggcgcgca actgatcaat caatcctggc gtgacgggat agatattgac
    17761 cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt tactggtcga
    17821 taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca atgtcagtac
    17881 cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg taatgttatg
    17941 aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg gatagagctg
    18001 gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg aaaccaccgc
    18061 gcggttgcgg ttaggtccaa tggtcatcaa gtcgaatgga atcgagatat aaaaaccttt
    18121 actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg cccatacccc
    18181 taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt tatcttttgc
    18241 cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg ggttccagta
    18301 agccgtcacg aaacctgttg gcgtggaata atcggtgaaa cgcatcatgt tgtcccagtc
    18361 acgttgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta gcgggcgata
    18421 aagcagctcg gaaccgacac cggcgtacat cgtttccaga taaccgccat acacctggcc
    18481 atagaaacca ttgcctaagt cggcaaagta gttcgcctgc aagttgttga gataaacgtc
    18541 attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt ccgcgggcaa
    18601 cagcgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac cgccatcaag
    18661 cagcaggtgg tcggtaaacc agtagcgggc actggacatc agccccagct ggaacatata
    18721 gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt agctaaagcg
    18781 atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt caacacgttg
    18841 ttgttgaagt tgctctgatt gaccaggcgc tgtaccagcc agctgtttgc gcaggctggc
    18901 tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca cgctaatctt
    18961 ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga ccgcttcgcg
    19021 agaatcacgg tatttgtatt gttgaccaga catatacagc gtcttatcgc gcagctgaat
    19081 ttccggtgct tcaaaacctg cgttgtactt cagcgcggta agttggttcg ctactgtggt
    19141 gtactgcaat ccttcagatt caggcgcagg ttgatatgcc ggttttggcg tatcgcgcag
    19201 ggcagggcgc agatcgttga aattggtccg taacgtgaag ccaaacatca acgtgttacc
    19261 gcgttcataa ctcaggttga gatctgccca gctggcagcg cgataaactg cgccgacgtt
    19321 gaaatggctt gcctgaggca gtttgccagc gaaatcattc tggtaattgt tgccatcgta
    19381 ttcgagtttc agacgcaggg gattccacgg cgtttgatac tcaatgccgc caaagatgga
    19441 agccgggcca cgaaagatat cgctaaagct gatatcgccc gcatcgtgag actctgcgcg
    19501 atgacaatat ttatcgctca cccggcaaaa cgggttggta atattgcccg cattaccggc
    19561 gtagccccat gccatcccga gggtgaaatc aaatggcccc gcttgcttgc tggccaccag
    19621 atactcaccg tcaaacagac ccgtaccagc aatatcacgt ttaccaaacg ccacttgcgg
    19681 tagccagtaa ccttcttccc acaggcgaag tttaaaatcg aatgatttgt ctttgtatga
    19741 ctgatcgccg ctgaaatctt cccactggct atatttgcgt gtgcgcacat ccgtataacg
    19801 aatggtgcct tccagccatg ggaacagcgc cacggaggtg gagtagaacc ggtattgatc
    19861 gttatcccgg tagttgacgc tgaattcacc ttccggtgcg atgcgcgcat ttggcatctg
    19921 caacaatcct gtgccgccaa agtctgattg cgacgaaccc agcggatccg ggtaggttaa
    19981 tacttcagca tgacaggcac tggagacggc aatggccagg cagcttaaaa gataagaatt
    20041 cttcttcatc agtcaggaac acgttgcgtt aaaacagaca caatctggtc atttaaatca
    20101 gcatattttt cgggcagtac atgcgcagaa aagcccaacc aaagctggct gccaggcggt
    20161 ggttccacat gtcttttatt ccacagggca acaggggcga cgacagtctc accctcaggc
    20221 gtaatcacca taacgttatt cttgtccgcg cctgcaaggc gggggtgatc ttgcagataa
    20281 tcggtaacgc tacggcctgc ctgccagggc aattgcccgg ccccagaaac cgcacccagc
    20341 agagtaatag tcaccggacg ctgtacggta tagagcgtat agtccccgac caacggggga
    20401 ttgctgttct catccactcg tacaaaatca gggtccagtt tgaccggtaa gcgaccagtg
    20461 atattgagat tgagtaactg ctggcgcacg gatttaatcg ttgccgccac atcgtcgtca
    20521 gcttctgctt cccaggaagc aagctgtgcc atgacatgtt ggtagtcttt aagcgcttta
    20581 gctttcgccg cagagtcggt cagcaaggca ccaggccacc agaggcgatc gcgcagttgc
    20641 ggttgtgtca ccaactggac aacattttcc atggggccaa cggaaagtgt ctgttgttcg
    20701 ccaggcagat aaatagtcac cgttccttgc gcaaaggcat ggggtgtcat tacgtaaagt
    20761 acgctggcaa tgaaatacga ctgtaattta ttcatggtgc gggtttaaga aacgtcattt
    20821 ctacgggaat aacccctgcg cctaacattt gccgactttg acgaacctgg ccagtagccg
    20881 aatcgaccca gaatgtgttt tgccactgtt tttctggccg cgtggaagtc acctcttcct
    20941 gccagacatt acacagcaca ttgcgacccg caatattcag cgtctctaat ccagcaaagg
    21001 aaaaagtaga actcagggtc gcagagcgaa aatggttgtc ttcactccag agaatatcgc
    21061 gcgtccagcg gctgccatct tttattgcca gcgcgttacg cagagggtcc tgcccggaat
    21121 ttgtcacttc cagcagatta ttattaagtt tgacggtttt taaaagacgt ccattgtggg
    21181 taaccagcat ggcgttatcc tgggataacc atttgctttg ttcttgttct atatatccca
    21241 gtaccacaaa gattcgttgc ccttcattaa ggcgtaaata catcgtggaa taaggcaacg
    21301 cctggatctg ctgatccgct acggtgatat cctgattatc gaaaaggctg gcgcgaaatg
    21361 tatcgaccat actttgctgg ctgtgcgtac atcccgcaag aaatagtgcg aaaatcgata
    21421 aaataagagg gcgcaccttt aatccttgaa aggttaatta aaaaaatgca aatctctaaa
    21481 gcccggtacg aatatcgcaa ccgggtatca tttaacttta atttcgacgc gttaaagcag
    21541 tattagcgtg tagtggacgt ggtggttgag gtgttatgag aaccgccatc gccactgtca
    21601 tttgctgcaa caactgccaa cgtcgccaca ccaacagccg ttgccacacc gacaactgtc
    21661 gccgtgccga cagcgccagc agccacgcca ccagtggtag tagcagtcgt ggttgcgccg
    21721 gtagtggtcg ttgtggtgcc agtagtaccc gtcgttgcct cggtcccgtt tgttgcttca
    21781 ggggcagcaa acgcagctgg cgtcgtcaac atgaaggcca taaggattgc agaaagcttg
    21841 tgtttcataa cttttccttt attcatcgca tggacaatac gtacgcttga gatcaggatt
    21901 gtctactttg agtataacat ccttgtgaaa tctccctttc ctcataccgc ttgcactata
    21961 tactgcgggt aggaaaggcg cgcagaggga aatataagat tgttaactat ccccctctgg
    22021 aaatacgact tgtgagcata agataaatct tagcaaaata caaattaaag ataaggaaat
    22081 tgttacgaaa gctattaatt attgttggta atatagtttc aagtggtact atagctggct
    22141 gttaaatggc atttataact attaggtgca gagatattcg cttaaatgga ttagtttctt
    22201 tgtggaaaag taactggtgt tataatgtat gatgttgact attgcactat tccaataaaa
    22261 ccagtcagct ttaaacaagc agcgtcatat taagagagat aaacatttgc cgctgttggt
    22321 cctcgcaggc catttacgcg gcaaaattcc acacgtaatc ctggtataag cacttctgcg
    22381 tcgcggggag tgaatgcgga aatatggacc tgaacttctt tacgaccgtc ggaggggata
    22441 atgaatcctt tgccgctttt gcgatcaaag gttttgacaa ttcctgtcat tttacgggac
    22501 aaacaaattc cttactgaaa atactgcgct gcactatacg gggttaataa aataaagcca
    22561 gcgatattta agaccgccgg acggctaaaa taaaatttgc ttaatctcaa ttatcatgcg
    22621 ttaatagctg cgtcggtttg aaagacagac agcatacaaa gtagtttact aaagcagttc
    22681 tcattatcag gcattatccc cttcttttga gtctctctcc tgaacactaa gtagtttctg
    22741 tattaaagcc ctgtttgccg aaaggcccaa aatgaaggaa gtaaaatatg tctaataaaa
    22801 tgactggttt agtaaaatgg tttaacgcag ataaaggttt tggctttatc actcctgatg
    22861 atggcagcaa agacgttttc gtccatttca ctgccatcca gagcaatgaa ttccgcacgc
    22921 tgaacgaaaa tcagaaagtt gaattttcta ttgagcaggg gcaacgtggc cccgcggcag
    22981 cgaacgttgt tacgctctaa ggttgccgtt attactcaac atctccattt ccgctgtcca
    23041 tgttgtcatg gttcacagta ccgtacatcg gcattcgatg tgacggagcg aaaccctttg
    23101 ggcgctaagt gtattttttg taaatcgacg atgatcacct ttgataacgt cgcgctgcaa
    23161 atacgcacgg accatgcgcc gctggatttc acaaaataat atcaggctcc ctcgtggagc
    23221 cttttttata tctgccttat ttttcttcaa cgctgtatgt atagtaagcg ataacctgtt
    23281 gattattgaa tctttcgggg agatggctta taacatttct tacctgacca gggtaccggg
    23341 aaccaacacc ttactggcgt gttgctgtct ttcaagacca gaagaggtta acagtgaata
    23401 ttgaagagtt aaaaaaacaa gccgaaacgg aaatcgccga ctttatcgcg caaaaaatcg
    23461 ccgagctgaa caagaataca gggaaagaag tctctgaaat tcgcttcacc gcacgagaaa
    23521 aaatgaccgg gcttgaaagt tatgatgtca aaatcaaaat aatgtgattt tgtgaacatc
    23581 acccgtgcga ggtgatgttc cgcttgttgc taatttagtg accaatcatt ggcgcttgtg
    23641 gaattaagcg tcggtacaat tcatccggta ccgggctttg ccatactccc gcatacattg
    23701 cgtaaccaat caccgcaaac ataatcccca gaaccagtag cgtcattaac cagccagaca
    23761 acgcaaaggc ttttttattt gccgcaggtt tttgcagtga aaaggtcaat gttgaggcta
    23821 ccggacatga ctctacgcaa gtcatacagc cggtacattc cactgttcgt acctgaatta
    23881 atttatcgac cgggatccgt gatgggcaat tttttgcgca tttgccacag tcgatacaac
    23941 tttcggcatt gcgacgaatc ttaaacggcg acaatagcga aaccacgccc atcagcgcgc
    24001 catatgggca aagataacga caccaggcat ggcgaataaa caggctggca atcagcaaaa
    24061 cggtcacgct gattaatgtc gcggtcccca tatgacgaaa gaaatcgagc attttaacgt
    24121 ccatcaccac gctgtagggc gacaacataa aatagtgaat cgcctgagcg ggcatcaata
    24181 atgcgatata gagaaaaaaa ctcaacagca aatacttcac gccgcgcaga ggaatatcca
    24241 gccagcgggg aaggacacat tgccgaccaa acagtttgtt accgagatcg ccgattaatt
    24301 cagaaagcgt accaaccggg cataaccatg agcaaaaggc ctttttgagt aatagactga
    24361 tgacgataaa agcgaccaat aacagcatcg cggcggcgtg gacggacggt aactgacctg
    24421 ttgtaaggct atatttcaga ttcatcagac cggcaatcgg tagccagcct tcgatacctc
    24481 ccggtctggc gacaaatgtc gtgctacttg ccgtttcgta atagcgcacc caataccaga
    24541 acgtgatggc aatataaata ttcattgcca acagtaataa ttgcgtcgct ttacgccagg
    24601 tcgtggcatt gcgccagtca ttccacggta atttgccgcc cgtcgtgcct ggccgccgct
    24661 gccagcgggt tctttttttc tctgccatga ttttgccagt ccgttaagtt gcataccaaa
    24721 tgccactatt ctagttgttc ttaactggct gatattgatt caaatcgcgt tcaggtcttt
    24781 cttattcaac catgcttcca gatcggcaac actgcgtgta atttcttcgt gtggaagggg
    24841 ggcagataat ggctgctgct ccagttgtgc gcatagctgg ctggcgatac gcattcccaa
    24901 actcgagcaa ctgcttttta gctgatgcgc ggcacgcttt attttctcgc tatcctgact
    24961 ggcgcgggca atgtcgattt catcgagaag cggcagggca tgttgtgtaa ataataccag
    25021 ccattcgtgg atcttctccg tccccattaa ctgagcatct tcattgagtt gcgatacatc
    25081 cagcgattga tcgttattga cttgcagttg gagatagtgc gccagtaact gaccgagcac
    25141 ttcacgcggc accggtttag ggataatccc gcggaataat gaactggtac gctggcgcag
    25201 cgtttcgtca atgacatggg cgctaaagcc aatcaaaacc agcgacggat attgctgtgc
    25261 cagttgtcgg gcaagcgtaa tgccgtcgac atccggcaga tcaaaatcca ccagtgcggc
    25321 agcaaacggt tcgctatttt gcagtgtctc taaagcctgc gcggcattgc caacagcaac
    25381 aacctgcgca ccactggtgt tcagcatctc aacggtaatt cgctgggtta gcgggttatc
    25441 ttcaattaac agcaaacgta aaccgtcaag acgcaccgcc tgattgactg tttttggcac
    25501 gggtgccgtg gcaacacgta acggcaagcg taaacaaaaa cagcttccaa cctccggcgt
    25561 gctggtggcg ctcagttcgc cgcccatcgc ctgggccaga cggctactga tagtcagccc
    25621 cagcccggtg ccgccgcgtt tgccgcttac ctgcacaaat ggctggaaga tttctgccag
    25681 tttcgcggga tcaataccgc agccgctgtc ttccacttcg accagccatt gctcgccatc
    25741 agtgcgacta cgcaggataa tgtacccttc gtcagtaaaa cgcagggcgt tgctcaacag
    25801 gttggttata acctgacgaa tacgtcgtgg atcgcccatt aacgcgcacg gcatatcatc
    25861 ggcaattgcc gttgccaggc gaatcgggcg acctttcacc cgtccgctca ttaattgcag
    25921 ggtactttcc agcagcgggc gcggttcaaa gggctcatcg ctgaccgaaa cattcttgcc
    25981 acctgcttcg atagcggaat aatcgagaat atcgttgagg atggtcagca acgattcacc
    26041 agagtcagta attgcccgca aatcatcacg ctgggcgtta agtgcggggt tatctgccag
    26101 cagttgagca gtgccgagaa taccgtacag cggtgtgcgg atctcatggc tcatcgccgc
    26161 cagaaacgcc gattttgcct ggctggcttt ttctgcttcc gcccgtgcct gtcggtgttc
    26221 tatcaccagt tcctgcaatt cagctgtacg cgctttgacc tgcgccgcca gctgttcgcg
    26281 gtggcgattc agtgcatgaa cattgctgcg aaacgcatcc atcagccgcc cgatggtatc
    26341 cagctcccgt acgccagcgg tttccgggaa aggggagtca atatcaccgt ccagcagccg
    26401 ttgcagcgcc tgcgtttgtt cggcaagtgg acgcgtgact gagcgataaa ccacgcgcca
    26461 gaggatcaga atcagtgcgc aaagagaaac tattcccagc aataacaggc tgtattgccc
    26521 gcgtgcactc gctttttcca gatgcgccag tccgtgctga ttacgcagct caatggtatc
    26581 gaccagctga ctgacttcgc tactaaactg cgcgaactgg gcgatgttat tttgggcgag
    26641 agtttgcagg tgagtgctga tttcactgtc ctgctgatac agcgccagta gatcgctata
    26701 ttggctaacg gtagttaacg ttgttgcgac ctgcgcacga actcccggat cttcaatgcg
    26761 tatttgccga cgttgcagaa ttttcaccgc attattgagc tgcttttcca gcgttggtgc
    26821 atttttctgg atctgctcca gccccagatt catcaccatt tgctgcaccc gcagagcgct
    26881 aaggcgcagt tcattcatct ggttaacata ctcaagatcg atatcaatca gccgatcgag
    26941 tgcactttca gcagcctgac gctgatcttg ttcgatcaaa tcgtaaatcc cggcctgggt
    27001 cgctccagcg gaagttgtcg cattattcgc ctgaccttgc gccaggcgtg cgatctcatc
    27061 ggcggcagcg actatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
    27121 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
    27181 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
    27241 ttgtgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
    27301 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
    27361 ggtaggaatc aatgcctgag tgttgtcttt ttccacctgg ctgataaagc gcaggttgta
    27421 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
    27481 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
    27541 aagcggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
    27601 atttttactt ctttcccttt tcatgttatc ggcattttcg gctgataacc tgttgcgctg
    27661 gcatgatgcg cagcattaca cggtgcaagc ctctacgccg cttaaagcca aacgcgcatg
    27721 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
    27781 tatgcaggag gcagctcgcc gctatggtgt ggatttaaaa gtgctggagg caggcggcta
    27841 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
    27901 cattttgctc ggtagtagca ctacgtcatt tcccgacctg caaaagcagg tagcaaatct
    27961 gccggtgatc gaactggtaa atgctcttga tgatcccctg gtgaaaagcc gcgttggtgt
    28021 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
    28081 accactgaac gtgctgttga tgcccggacc cgataatgcc gggggcagta aagagatggt
    28141 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
    28201 tgataacgat attgaaatcc agcgtaacct gttgcaggag atgctggaac gccacccaga
    28261 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
    28321 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
    28381 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
    28441 ggttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
    28501 gattttagtt ctgacgccga aaaatgccga ccgtgaacat attcgccgct cgctgtcacc
    28561 agggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
    28621 accatgttgc gtcaccagta aatccgcgct gagtttatga cgtaaacgac gaattaacac
    28681 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gtagcagacg
    28741 ttcacggctg agaatttcgc ccggattcgt cacaaatgcc accaacattt catactctgc
    28801 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
    28861 ataaccggca aagcgatagc agttgtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
    28921 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
    28981 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
    29041 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
    29101 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
    29161 ttcccgcagc ccggcaccgc tcgctgtaac ggaaacggta tacccctcct gagtgaagta
    29221 agattgtaat cgcgcctggg taaccggctc atcttcaaca ataacaatgt gatgtggcat
    29281 cagagggttt tactcattct gttcatatct gttcatattc tgccgtaagc cgttcatcct
    29341 gaccagtgcc gctgttcata tttgctcatt aagatcgctt cactaaacca taattttaca
    29401 ggggctatta tgcggaaact ctggaacgcg ctacgccgac ccagtgctcg ttggtcggta
    29461 ctggcgctgg tcgcaattgg gattgtgatt ggcattgcgc tgattgtatt gccacacgtt
    29521 gggatcaaag tcaccagcac aaccgaattt tgtgtcagtt gccacagtat gcaaccggtg
    29581 tatgaagaat ataaacagtc ggtgcatttc cagaacgcct ccggcgtgcg agctgaatgc
    29641 catgactgtc atatcccgcc ggatattcca ggcatggtga agcgcaaact ggaagcgagc
    29701 aatgatatct accagacctt tattgctcac tccattgata cacctgaaaa attcgaagcc
    29761 aaacgcgcgg aacttgccga gcgtgaatgg gcgcgaatga aagaaaacaa ctcggcaacc
    29821 tgccgctcct gccataacta cgatgcgatg gatcatgcga agcagcatcc tgaagcggca
    29881 cgtcagatga aggtggcagc gaaagataat caatcctgca tcgactgtca taaaggtatt
    29941 gcccaccagt taccggatat gagtagcggc ttccgtaagc agttcgatga gctgcgcgcc
    30001 agtgctaatg acagtggtga cacgctgtac tctattgata ttaagccaat ttatgcggcg
    30061 aaaggcgata aagaagcctc tggttctctg ctgcctgctt cggaagtgaa agtccttaaa
    30121 cgtgacggcg actggctgca aattgaaatt accggctgga cggaaagcgc cggacgtcag
    30181 cgtgtactca cccaattccc aggtaaacgc atctttgttg cctcgattcg tggtgatgtg
    30241 cagcagcagg taaaaacgct ggagaaaacc accgttgccg acaccaatac cgagtggagc
    30301 aagttgcagg ccactgcgtg gatgaagaaa ggcgacatgg tgaacgatat caaaccgatc
    30361 tgggcttatg cggattcgtt gtacaacggc acctgtaacc agtgccacgg tgcaccggaa
    30421 atcgcccact ttgacgctaa cggttggatc ggcacgctca acggcatgat tggctttacc
    30481 agtctcgata aacgtgaaga acgcaccttg ttgaaatatc tgcaaatgaa tgcgtctgac
    30541 accgcaggta aggctcacgg cgataagaag gaagaaaaat aatgaacaat aacgatctct
    30601 ttcaggcatc acgtcggcgt tttctggcac aactcggcgg cttaaccgtc gccgggatgc
    30661 tggggccgtc attgttaacg ccgcgccgtg cgactgcggc gcaagcggcg actgacgctg
    30721 tcatctcgaa agagggcatt cttaccgggt cgcactgggg ggctatccgc gcgacggtga
    30781 aggatggtcg ctttgtggcg gcaaaaccgt tcgaactgga taaatatccg tcgaaaatga
    30841 ttgccggatt gccggatcac gtacacaacg cggcgcgtat tcgttatccg atggtacgcg
    30901 tggactggct gcgtaagcgc catctcagcg atacctccca gcgcggtgat aaccgttttg
    30961 tgcgcgtgag ctgggatgaa gccctcgaca tgttctatga agaactggaa cgcgtgcaga
    31021 aaactcacgg gccgagtgcc ttgctgaccg ccagtggttg gcaatcgacg gggatgttcc
    31081 ataacgcttc ggggatgctg gcgaaagcta ttgccttgca tggtaatagc gttggtacgg
    31141 gcggagatta ctctaccggt gctgcgcagg tgatcctgcc gcgcgtggtt ggctcaatgg
    31201 aagtgtatga acagcaaacc tcctggccgc tggtattgca gaacagcaaa accattgtgc
    31261 tgtggggctc tgatttgctg aaaaaccagc aagcgaactg gtggtgcccg gatcacgatg
    31321 tttatgaata ttacgcgcag ctaaaagcga aagtcgccgc cggtgaaatt gaggtcatca
    31381 gcatcgatcc ggttgtcaca tccacccatg agtatctggg gcgcgagcat gtgaagcaca
    31441 ttgcggttaa cccgcaaact gatgtgccgc tgcaactggc gctggcgtat acgctgtaca
    31501 gtgaaaacct gtacgacaaa aacttcctcg ctaactactg tgtgggtttt gagcagttcc
    31561 tgccgtacct gctgggtgag aaagacggtc agccgaaaga tgccgcatgg gctgaaaaac
    31621 tgaccggcat tgatgccgaa accattcgtg ggctggcgcg gcagatggcg gcgaacagaa
    31681 cgcaaattat tgctggctgg tgcgtgcagc gtatgcagca cggtgaacag tgggcgtgga
    31741 tgattgtggt tctggcggcg atgctggggc aaattggcct gccaggtggt ggctttggtt
    31801 ttggctggca ctataacggc gcaggcacgc cggggcgtaa aggcgttatt ctgagtggtt
    31861 tctccggctc tacgtcgatt ccgcctgttc acgacaacag tgattacaaa ggctacagca
    31921 gcaccattcc gattgcccgt tttatcgatg cgatcctcga accggggaaa gtgatcaact
    31981 ggaacggtaa atcggtaaaa ctgccgccgc tgaaaatgtg tatttttgcc ggaactaacc
    32041 cattccatcg ccatcagcag atcaaccgca ttattgaagg ctggcgcaag ctggaaacgg
    32101 ttatcgccat agataaccag tggacctcaa cctgccgctt tgccgatatc gtgctgcctg
    32161 cgaccacgca gtttgagcgt aacgatctcg accagtacgg caaccactcc aaccgtggca
    32221 ttatcgccat gaaacaggtg gtgccgccgc agttcgaggc gcgcaacgac tttgatattt
    32281 tccgcgagct gtgccgtcgc tttaatcgcg aagaagcctt taccgaaggg ctggacgaaa
    32341 tgggctggct gaaacgcatc tggcaggaag gtgtacaaca aggcaaagga cgcggcgttc
    32401 atctgccagc gtttgatgac ttctggaata acaaagagta cgtcgagttt gaccatccgc
    32461 agatgtttgt tcgccaccag gcattccgcg aagatccgga tctcgaaccg ctgggcacgc
    32521 cgagtggcct gattgagatc tactcgaaaa ccatcgccga tatgaactac gacgattgtc
    32581 aggggcatcc gatgtggttt gagaaaatcg aacgctccca cggtgggccc ggctcgcaga
    32641 agtatccgtt gcatctgcaa tctgtgcatc cggatttccg acttcactcg cagttatgtg
    32701 agtcggaaac tctgcgtcag caatatacgg tagcgggtaa agagccagtg ttcattaacc
    32761 cgcaggatgc cagcgcgcgc ggtattcgta acggtgatgt ggtacgcgtc tttaacgctc
    32821 gcggtcaggt gttggcaggg gcagtagttt ctgaccgcta tgcacccggc gtggcacgaa
    32881 ttcacgaagg ggcatggtac gatccagata aaggcggcga gcctggtgcg ctgtgcaaat
    32941 acggtaaccc caacgtgctg accatcgaca tcggtacttc gcagctggcg caggcgacca
    33001 gtgcgcacac tacgctggtg gaaattgaga agtacaatgg agcagtggag caggtgacgg
    33061 cgtttaacgg ccctgtggag atggtggcgc agtgcgaata tgttcccgcg tcgcaggtga
    33121 aatcatgacc acgctgacag cacaacagat tgcctgtgtt tacgcctggc tggcgcagtt
    33181 gttctcccgt gaactggacg atgaacaact gacgcaaatc gccagtgcgc agatggctga
    33241 atggttttcg ttgctgaaaa gcgaaccgcc gctggctgcg gcggtgaatg agctggaaaa
    33301 ctgtattgcc acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg
    33361 cggcctgttt ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca
    33421 ggacgagcaa gagattaaac gcttgttagt tgaggcaggg atggaaacca gcggcaattt
    33481 caacgaaccg gcagatcatc tggcgatcta tctcgaattg ctcagccatc tgcatttttc
    33541 gctgggagag gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac
    33601 ggcgctgtgg caatggttac cagagtttgt tgtgcgttgt cgtcagtatg acagctttgg
    33661 tttttacgcg gcactaagcc agttattgct ggtgttagtg gagagcgacc accaaaacag
    33721 ataacgtcgt ttgtgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggggca
    33781 gttgccggat gcgtcatcac atccggcaaa cacacatcac ggatgagtta caaaccggga
    33841 aagtcgctgg cgcagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt
    33901 cagcgccacc gcgatcccag gccagtccag agccagttca tgacgcaggc gtactgcgcg
    33961 ttgcaccacg atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg
    34021 ttcaaccacc cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag
    34081 gcaaaattcg gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct
    34141 ttacgtggat caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt
    34201 tcatccggtt tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt
    34261 ttgctcacca gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg
    34321 atagtcagca aaatgctttc tttcagtgtt ggaacggtga ctttagcacc cagcgccgct
    34381 tcccacgggc taaccggcac tacaatttcc agatcatggc cgacaatatc gaacagcgga
    34441 tgtggcgcaa tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc
    34501 gtcccctggc ctttcagacg gatgcgttga ccattgccga cgcccgccgg gatcttgaca
    34561 ttcagcgttt tcggaatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta
    34621 tagctgatgg tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt
    34681 tcaatatcgt ggccgcgtgt ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa
    34741 attgacgaga agatatcgtc aaaatcttcg gcgttaaaac tctgaccgtc gctatgatgg
    34801 aactgacggt taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga
    34861 cgttgttcat cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca
    34921 tccggttctt tgctgacatc aggatggtat ttgcgggcca gtcgacgata ggcggtcttg
    34981 attgtcttga gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc
    35041 atagcgttat ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat
    35101 aagtgtaggg taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc
    35161 gcggagagga ggtcgcctca aggtggggtg ctgaacatat tttaaacagg tgaaaaaggg
    35221 tgagcgattt ttgatagtgg aaccaggcac tttaagttta actggggcgt cattatttat
    35281 taaattttat aggcgctata tatgagtagt aatatacatg gaattagttg cactgcaaat
    35341 aattatttga aacaagcctg gaacaatata aaaaatgagc acgaaaaaaa tcaaaaatat
    35401 tcaatcacgc tttttgaaaa cacactggtg tgttttatgc ggttgtataa agaaatcaga
    35461 cgtcaaaaag ctgaagacta cattccatgc ctggaatgcg attcactaga aaaagaattt
    35521 gaggaaatgc agaatgataa tgatttatca ttatttttga gaacattgcg tactaatgat
    35581 acagaaactt attcaggggt ttcagaaggt attacatata ctatacaata tgttcgagat
    35641 attgatattg ttagagtgtc cttgccgggc agaggttcag agtctatcac agattttaaa
    35701 ggttattatt ggtatggctt tatggaatat attgaaaaca ttaatgcgtg tgatgatgtt
    35761 ttttctgagt attgtttaga tgatgaaaat atgagtatcc agccagaatg gataaatacg
    35821 ccgggaatat ctgatttaga tactggtatt gatttgtctg gtatatcttt tattcagagt
    35881 gaaattaata aaacttatgg attaaaatac gctccagtag atggcgatgg ctattgtctg
    35941 ttaagggcca tactggtttt aaaagaacat gagtattcat gggcgctggg cagccataag
    36001 acgcaaaagc aagtatacga agagtttatt aaaattgttg ataaacaaac gatcgaggct
    36061 cttgttgata cggcattcaa tgatctcagg gaagatgtaa agacattatt cggcgttaat
    36121 ctacaatctg acaacaaaat tcaggggcag ggtggttttt tatcatggag ttttttgtct
    36181 tttaaaaaag aatttattga tagttgcttg aatgacaaaa aatgcatact gcatttaccc
    36241 gagtttatat ttaatgataa taaggcacgc cttgttttag acaccgaccc agagcagaag
    36301 gttaatgagg tgaaaaattt cctcactgct ctttcagata gcatttgctc attatttatt
    36361 gttaatggta atgtggcatc aatctcctta gggaatgaat ccttttcaac agatgacgac
    36421 cttgagtatg ggtatttaat aaacactggc aatcattatg acgtttacct ccctcctgaa
    36481 ctttttgctc aggcttacga gttaaacaat aaggaaagga atgcgcaaat tgatttttta
    36541 actcgttatg caatttactg acaaaggcat gtgctaaaaa ccattgttat tagcctcact
    36601 tctcttatta ttggtactat tgcctctgta ttagtaacgc cgctgatatt ctgtttagcc
    36661 acaggtgtaa tcatcagcgg cgtacgcgag gcagggagct aatcaggcat agtttgcgtc
    36721 aaaccttgcc tgtttttaaa gatgtatata gaaaaacagg cattcaacaa gccattttgc
    36781 gaacctgttc ccggaaaaat gtcatatttc tgtcacactc tttagtgatt gataacaaaa
    36841 gaggtgccag gaatgaacaa aacgctaatc gccgcaactg tggcagggat agttttactc
    36901 gcttcaaatg ctcaggcaca aaccgtaccg gaaggctatc agctacagca agtgctcatg
    36961 atgagccgcc ataacttacg tgcgccgctg gcgaacaatg gcagtgtgct ggagcagtct
    37021 acgccgaata aatggcccga atgggacgtc cccggtgggc aactcaccac caaaggtggc
    37081 gtgctcgaag tgtatatggg ccattacatg cgtgaatggc tggcacagca ggggatggtg
    37141 aaatcggggg aatgcccgcc gccggacacc gtttatgcct atgccaatag tctgcaacgt
    37201 accgttgcga ccgcacagtt ctttattacc ggcgcattcc cggggtgtga tattcctgtg
    37261 catcaccagg aaaaaatggg cactatggac ccaaccttta acccggtgat caccgatgat
    37321 tccgccgcat tcagtgaaca ggcggtggcg gcaatggaga aagagctcag caaactccag
    37381 cttaccgaca gctaccaact actggaaaaa atcgttaact ataaagattc ccctgcctgt
    37441 aaagagaaac aacagtgttc gctggtggat ggcaaaaata cctttagcgc caagtatcaa
    37501 caagaaccag gtgtttccgg gccgctgaaa gtcggcaact cgctggtaga tgcgtttact
    37561 ttgcaatatt acgaaggttt tccgatggat caggtggcct ggggagaaat caaatctgac
    37621 cagcagtgga aggtgttgtc gaagcttaaa aacggctacc aggacagcct gtttacctca
    37681 ccggaagtgg cgcgcaatgt tgcgaaaccg ctggtcagtt atatcgacaa agctctggtc
    37741 accgatcgca ccagcgcacc gaaaattaca gtgttggttg ggcacgactc caacattgcc
    37801 tctctgttaa cggcgctgga tttcaaaccg tatcagttgc atgaccagaa cgaacgcacg
    37861 ccgattggcg gcaaaatcgt tttccagcgt tggcatgaca gcaaagccaa tcgcgatttg
    37921 atgaaaattg aatatgtgta tcagagtgcg gaacagttac gtaatgccga tgcgttaacc
    37981 ctgcaggcac ctgctcagcg tgtgacgctg gaattaagcg gttgcccgat agatgccaac
    38041 ggtttctgcc caatggataa gtttgatagc gtgttgaatg aggcggtgaa ataacagaaa
    38101 actcccccgc gagaagtggg ggagtcgctg gttaaacgtt tttacgttcg atggtctgtt
    38161 cgccccaaaa aagcgaatct ttatcggtct tagcaaaggc tttgactaac acttcatcgc
    38221 taccttcttc ccaaatcttt tccgccattt tttcgtcgta cccggcgact tcgaaaatgg
    38281 cctcggctat ttccggcgac gtattgcgca gagatgccca ttcaccgacg tgatgagctt
    38341 tcgcttcttg agttggcatg cgtatcctcc tgttgaagat tagccgttaa gtttaactgc
    38401 cagacctgcg acatattccc cttgataacg agcaatagac agttcttcct ggcttggctg
    38461 gcgtgagccg tcaccgcctg cgatggtggt tgcgccgtac ggcgtaccgc cgcgaacctg
    38521 tgaaacgtca aataattcct gcgctgcgta gccaatgggg acaattacca tgccgtgatg
    38581 cgcaagggtc gtccaggttg aagtaatagt ttgttcctga ccgccgccag taccggtgga
    38641 actaaagacg ctcgccagtt ttccgtatag tgcgccggaa gcccacaggc cgcccgtctg
    38701 gtcgaggaag gtacgcattt gaccggacat gttgccaaag cgggtaggtg taccaaaaat
    38761 aatggcgtcg taatcggcca gttcttgcgg ggttgcaacc ggtgcagttt gcgttttacc
    38821 gcctgctttt tcaaataatt gcggcggcat ggtttccggt acacgcttaa cgacaacttc
    38881 tgcgccatcg actttgcttg caccctcagc gactgcgcgt gccatcgttt caacatgact
    38941 aaggatatgg tttatcattt tgaaaaatat aattttattt catcctcctg gtcactttgg
    39001 ggcacgtctg gggcacgggc attaaggaca ttattcaaca tggcaacctg agtcacgctg
    39061 cactcaggca tccatgcacc ataaacattg tagaccatgc tggcgctgga gtgccccatc
    39121 tgtgatgcaa taaatgtcgg gtttgctccg gcagataaag cccagcacgc ataggtatgg
    39181 cgtgactgat acgctttacg ggatcggata cccgctcttt ttattgctga atcccatgtc
    39241 gctccgatgg agcttaccgc gtagttaata cccgccttgt gattcttgcg aacgatttgc
    39301 ggacagaaaa caaaagtgca ctcgtgcaaa attgttcttc cgtactcgcg taattgaaca
    39361 gtgatctgat gctgcctgct aagacgagta agcatcgcct ggtttttaag tgcttcaatt
    39421 gctggtgcca gaagatgtat aacccggtta gtgcctgcgt cggtctttgg tagcgtaaaa
    39481 tcacctattt ttgtaaaatt tcgtcgcact gttatcgtgc cagctttcag gtcgatatcc
    39541 tcccatgcaa gtgcggcaat ttcaccgtgt cgcatccctg taaaaacagc cactgtccag
    39601 aggtttttgg tctgttgatg atggcaggca tcaatgagac ggctaaactc atctctggtc
    39661 agtggatccg gcactggttt tgatttcctc agcggtgtta ttgaattaaa ggggtttttc
    39721 tccagatacc cgttttcggc ggcaaagctg aacattccgg ctgttgttgt catgtaatag
    39781 ttcactgtgg gtacggttct tccttttcgg gacgtgctgg ttttcctgct tcccttttcc
    39841 ccggtcagta aatctttcct gataaacagc agatcttctt ttgtaatcga tgacgccagc
    39901 ctgccaggac caagcctcgg tagcatattt ttcatcactg attgataacg atttaaggcg
    39961 ttactactga tttccattac tttcagagta agccatttct gtgccagttc accgacggtg
    40021 atatcttttt ttaccaggcc aaatagcttc aggttaggtg agtcagggaa tcgatcggca
    40081 taatcaaacg ttcctgttct gattgcaaag cagaccgaag cccttaactc accagcgatc
    40141 tttctgtttt tcggtgtatc gggcacgcga agattttcgc gcactcgttt acccctgtac
    40201 ttaaacgtta ttcggagttt tcctccgtga ttttcaacgc cggctggata ggctgaattc
    40261 gccattgttc ctcctgcgtc caagagcacg ctcaggttat actcttatgg acaagaatta
    40321 aacatcctga gatggtaagg gttggttttt gatccaagtg ttgatggtag gaagatgata
    40381 gagacattcg ctgtttttct ttggcgttcc gtcgggggcg atttgtttat attctctgcc
    40441 gttcatccac gcactgtccc tggcgcgtaa tattgttcct tttctcaggc ctgttacagc
    40501 cattaaaagc tccagtgtta cccattcatt cgcatttatc tgtacaacag gtgtgatgac
    40561 tggctgaatg ccatggttat gttggtttac taactcgcgc ataagttgcc tctgcatgtg
    40621 taagaaaaaa ccgcccgaag gcggttgtca gttgattgat gtacggcgca tttttcgaag
    40681 gctggcaata tgcttttcct tctcaatttc cgctttaatc atatgcagtt cgttgtgctc
    40741 gattcgctca aattcttcat taaatgcacc aattgaagtg gctctggttc tgccgtcgag
    40801 ccttcggtag atcacctgag tcagagttac cttgcatatt tctaccggat agttgttggc
    40861 atcaacgaaa gactgcccgc gctggatcag gacgaacact ggttgtattc cttgagtgcc
    40921 atatcataaa cacggcatgc cgagaggtgt gttgtagtca acgtcgttcg tatcctgaaa
    40981 agtccatgtg ttgtgacggt aacttccggg gctttgagtt tcactgcatc aacgatatgc
    41041 tgatgtttgc ggctttcgaa tatagagctg gagataacca cacttgctgt taaaccgtaa
    41101 ctttcaaatg tgattttcat gtcattatcc tcgcagttag ccgggcaaca aaaataaata
    41161 atgttgttcc tgcgaataac aggataccgc ccgtcattag agaaaggaaa aacaatatta
    41221 atgtacgcgg gttgtttctc aaaatattct ctctgtgatt cgctcactct gacaattcgt
    41281 tgatcaagtc acggtattta ttcagttctt gcagcgcctt acatgcaccc tcccatcgtt
    41341 tctgtttccg cccggcgcga cgcgcttctt tccgtgattt acgtagcagg tcacctataa
    41401 tgttgtgctg cactggctgc actggctgca ctggctgcac tggctgcact gaggcgatat
    41461 ttggtttttt gtccacttcc cttgcaactg cctgccagat gtcatttttt acagttacta
    41521 ccccctggtt ttttaacttc catagagtgt caataacgtc attcaaatca atattgagac
    41581 ctttagaaat actttcggct gatgcttttc ctaatttgtt caattctgtg agcactgcgt
    41641 tcattttttt ctcctgatat tttaatgtcg gtggggctgt tatacggttg gtgaggtgtc
    41701 agcttcttct accagctttt ccagttcatc cagcttgcgg gaaagaattt caccaaacag
    41761 atgaagttct gcatctgctg taatcgggat tggaacaaat cgtattccac ttctggcaag
    41821 ttgcaggtta gccacggtta acctcctgcg gcggttccgg tagcggcatc cagtgtgtgg
    41881 cctgttcaat accattacca tcaataccat taccaggcgc aaccgttatc tgcccacgcc
    41941 gaaaggtgct tcctgtatag cgtgcggagc atattagcgg ttcaaccaga gatctatcga
    42001 aattcaccga aataagcaca ttctggttct tttcaggcat tcgctcacta cagcttatcc
    42061 aaccatccgg agttaccgta gagttgcccg atagtgcatt ctgctccagt gatgctttta
    42121 caaaccacgc tgcctgaact ataacgccat g
//
LOCUS       DAZFJF010000027        41462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFJF010000027 DAZFJF010000000
VERSION     DAZFJF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.027"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..206)
                     /locus_tag="ACHKAU_001647"
     CDS             complement(<1..206)
                     /locus_tag="ACHKAU_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530734.1"
                     /translation="MSGKPAARQGDMTQYGSSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGP"
     gene            complement(366..578)
                     /gene="rpmE"
                     /locus_tag="ACHKAU_001648"
     CDS             complement(366..578)
                     /gene="rpmE"
                     /locus_tag="ACHKAU_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO5530735.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            781..2979
                     /gene="priA"
                     /locus_tag="ACHKAU_001649"
     CDS             781..2979
                     /gene="priA"
                     /locus_tag="ACHKAU_001649"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO5530736.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3135..4160
                     /gene="cytR"
                     /locus_tag="ACHKAU_001650"
     CDS             3135..4160
                     /gene="cytR"
                     /locus_tag="ACHKAU_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO5530737.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4252..5211
                     /gene="ftsN"
                     /locus_tag="ACHKAU_001651"
     CDS             4252..5211
                     /gene="ftsN"
                     /locus_tag="ACHKAU_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO5530738.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5304..5834
                     /gene="hslV"
                     /locus_tag="ACHKAU_001652"
     CDS             5304..5834
                     /gene="hslV"
                     /locus_tag="ACHKAU_001652"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO5530739.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5844..7175
                     /gene="hslU"
                     /locus_tag="ACHKAU_001653"
     CDS             5844..7175
                     /gene="hslU"
                     /locus_tag="ACHKAU_001653"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO5530740.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7242..8168
                     /gene="menA"
                     /locus_tag="ACHKAU_001654"
     CDS             7242..8168
                     /gene="menA"
                     /locus_tag="ACHKAU_001654"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO5530741.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8261..8746
                     /gene="rraA"
                     /locus_tag="ACHKAU_001655"
     CDS             8261..8746
                     /gene="rraA"
                     /locus_tag="ACHKAU_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO5530742.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8831..9076)
                     /gene="zapB"
                     /locus_tag="ACHKAU_001656"
     CDS             complement(8831..9076)
                     /gene="zapB"
                     /locus_tag="ACHKAU_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO5530743.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9501..10346
                     /gene="glpF"
                     /locus_tag="ACHKAU_001657"
     CDS             9501..10346
                     /gene="glpF"
                     /locus_tag="ACHKAU_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO5530744.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10369..11877
                     /gene="glpK"
                     /locus_tag="ACHKAU_001658"
     CDS             10369..11877
                     /gene="glpK"
                     /locus_tag="ACHKAU_001658"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO5530745.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12012..13022
                     /gene="glpX"
                     /locus_tag="ACHKAU_001659"
     CDS             12012..13022
                     /gene="glpX"
                     /locus_tag="ACHKAU_001659"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5530746.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13119..13865
                     /gene="fpr"
                     /locus_tag="ACHKAU_001660"
     CDS             13119..13865
                     /gene="fpr"
                     /locus_tag="ACHKAU_001660"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO5530747.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13870..14298)
                     /gene="uspD"
                     /locus_tag="ACHKAU_001661"
     CDS             complement(13870..14298)
                     /gene="uspD"
                     /locus_tag="ACHKAU_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO5530748.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14325..14624)
                     /gene="yiiS"
                     /locus_tag="ACHKAU_001662"
     CDS             complement(14325..14624)
                     /gene="yiiS"
                     /locus_tag="ACHKAU_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO5530749.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14836..15276)
                     /gene="yiiR"
                     /locus_tag="ACHKAU_001663"
     CDS             complement(14836..15276)
                     /gene="yiiR"
                     /locus_tag="ACHKAU_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5530750.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15377..15976
                     /gene="yiiQ"
                     /locus_tag="ACHKAU_001664"
     CDS             15377..15976
                     /gene="yiiQ"
                     /locus_tag="ACHKAU_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO5530751.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16084..16851
                     /gene="tpiA"
                     /locus_tag="ACHKAU_001665"
     CDS             16084..16851
                     /gene="tpiA"
                     /locus_tag="ACHKAU_001665"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO5530752.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16906..17661)
                     /gene="cdh"
                     /locus_tag="ACHKAU_001666"
     CDS             complement(16906..17661)
                     /gene="cdh"
                     /locus_tag="ACHKAU_001666"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO5530753.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17768..18757)
                     /gene="sbp"
                     /locus_tag="ACHKAU_001667"
     CDS             complement(17768..18757)
                     /gene="sbp"
                     /locus_tag="ACHKAU_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO5530754.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19076..20038)
                     /gene="pfkA"
                     /locus_tag="ACHKAU_001668"
     CDS             complement(19076..20038)
                     /gene="pfkA"
                     /locus_tag="ACHKAU_001668"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO5530755.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20219..21121)
                     /gene="fieF"
                     /locus_tag="ACHKAU_001669"
     CDS             complement(20219..21121)
                     /gene="fieF"
                     /locus_tag="ACHKAU_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO5530756.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21270..21770)
                     /gene="cpxP"
                     /locus_tag="ACHKAU_001670"
     CDS             complement(21270..21770)
                     /gene="cpxP"
                     /locus_tag="ACHKAU_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO5530757.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21920..22618
                     /gene="cpxR"
                     /locus_tag="ACHKAU_001671"
     CDS             21920..22618
                     /gene="cpxR"
                     /locus_tag="ACHKAU_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO5530758.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22615..23988
                     /gene="cpxA"
                     /locus_tag="ACHKAU_001672"
     CDS             22615..23988
                     /gene="cpxA"
                     /locus_tag="ACHKAU_001672"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO5530759.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24130..24804)
                     /gene="yiiM"
                     /locus_tag="ACHKAU_001673"
     CDS             complement(24130..24804)
                     /gene="yiiM"
                     /locus_tag="ACHKAU_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO5530760.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24953..25936)
                     /gene="kdgT"
                     /locus_tag="ACHKAU_001674"
     CDS             complement(24953..25936)
                     /gene="kdgT"
                     /locus_tag="ACHKAU_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO5530761.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26196..26816)
                     /gene="sodA"
                     /locus_tag="ACHKAU_001675"
     CDS             complement(26196..26816)
                     /gene="sodA"
                     /locus_tag="ACHKAU_001675"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO5530762.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27101..28135
                     /gene="rhaT"
                     /locus_tag="ACHKAU_001676"
     CDS             27101..28135
                     /gene="rhaT"
                     /locus_tag="ACHKAU_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO5530763.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLEKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28132..28980)
                     /gene="rhaR"
                     /locus_tag="ACHKAU_001677"
     CDS             complement(28132..28980)
                     /gene="rhaR"
                     /locus_tag="ACHKAU_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO5530764.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29054..29891)
                     /gene="rhaS"
                     /locus_tag="ACHKAU_001678"
                     /pseudo
     CDS             complement(29054..29891)
                     /gene="rhaS"
                     /locus_tag="ACHKAU_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            30179..31648
                     /gene="rhaB"
                     /locus_tag="ACHKAU_001679"
     CDS             30179..31648
                     /gene="rhaB"
                     /locus_tag="ACHKAU_001679"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO5530765.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31645..32904
                     /gene="rhaA"
                     /locus_tag="ACHKAU_001680"
     CDS             31645..32904
                     /gene="rhaA"
                     /locus_tag="ACHKAU_001680"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO5530766.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32987..33811
                     /gene="rhaD"
                     /locus_tag="ACHKAU_001681"
     CDS             32987..33811
                     /gene="rhaD"
                     /locus_tag="ACHKAU_001681"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO5530767.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            33883..35031
                     /gene="fucO"
                     /locus_tag="ACHKAU_001682"
     CDS             33883..35031
                     /gene="fucO"
                     /locus_tag="ACHKAU_001682"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5530768.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            35028..35342
                     /gene="rhaM"
                     /locus_tag="ACHKAU_001683"
     CDS             35028..35342
                     /gene="rhaM"
                     /locus_tag="ACHKAU_001683"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO5530769.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35655..36101
                     /gene="frvA"
                     /locus_tag="ACHKAU_001684"
     CDS             35655..36101
                     /gene="frvA"
                     /locus_tag="ACHKAU_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO5530770.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            36112..37563
                     /gene="frvB"
                     /locus_tag="ACHKAU_001685"
     CDS             36112..37563
                     /gene="frvB"
                     /locus_tag="ACHKAU_001685"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO5530771.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37553..38623
                     /gene="frvX"
                     /locus_tag="ACHKAU_001686"
     CDS             37553..38623
                     /gene="frvX"
                     /locus_tag="ACHKAU_001686"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5530772.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            38623..40371
                     /gene="frvR"
                     /locus_tag="ACHKAU_001687"
     CDS             38623..40371
                     /gene="frvR"
                     /locus_tag="ACHKAU_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO5530773.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(40421..41461)
                     /gene="yiiG"
                     /locus_tag="ACHKAU_001688"
     CDS             complement(40421..41461)
                     /gene="yiiG"
                     /locus_tag="ACHKAU_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO5530774.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
BASE COUNT        10071 a        10721 c        10733 g         9937 t
ORIGIN      
        1 gggccgggca gggcgatgtc ggtttcaccg ggaaggacct ttgcaccgag cagcggattg
       61 accggatggc cggacgtcac tccgccgggg cacaccgaac aggccacgcc ggtgggggca
      121 ccaatgcgca ccccagctga accctggaca atgctactgc catactgcgt catgtcgccc
      181 tgacgcgctg ccggttttcc gctcatcgta aaatcctttt gaaataaata agaaccagaa
      241 gcctagcgcc agtcaacaac aggaaatgat aatgagttaa caccccggaa tttttcagaa
      301 tttataaaag gcaaattaca ggcacaaaaa aagcgccgtg cggcgctttt ttcggaaatc
      361 cggtcttatt tgctgcccgg gatgttgaaa cgcttgttga agcggtcaac acggccaccg
      421 gtagcaacat cacgctgttt gccagtgaag aacgggtggc acttgctgca cacgtcgagg
      481 ttcaggtcat gaccaacggt ggagcggatt ttcattacgt taccgcaaga gcagctagca
      541 gtaatttctt cgtatttcgg gtgaatatct tttttcatgg gaaaacctcg gtttaaggcc
      601 gcgtcgctct tccagcccta acgccagaca ccacgcgatg ttaaaagtat agcttcaata
      661 cgatcatttc gtacgaaggc gcgaaatcat acagaaatta accagcgtat gcaaactgat
      721 ccgcactctt ctacggcaat gtgtatacta acccaccgaa tttcaagtca ggatgatgct
      781 atgcccgtag cccacgttgc cttgcccgtt ccgcttcctc gtacctttga ctatctgctg
      841 ccagaaggca tgacggttaa agctgggtgt cgcgtgcgcg tgccgtttgg caaacagcag
      901 gagcgcatcg ggattgtggt atcagttagc gatgccagcg aactgccgct caatgagcta
      961 aaagcggtag tcgaagtgct ggacgttgag ccggtattta ctcactccgt ctggcggtta
     1021 ctgctctggg cggcagatta ctatcatcat ccgattggcg atgtgctgtt tcatgccttg
     1081 ccgattttac tgcgccaggg gcggcctgcg gcgaacgcgc cgatgtggta ctggtttgcc
     1141 actgaacaag gtcaggcggt ggatcttaac agcctgaaac gttcgccaaa gcaacaacag
     1201 gcgctggcgg cgttacggca aggcaaaatc tggcgcgacc aggtcgcgga acttgaattt
     1261 aatgatgccg cgttgcaggc gctgcgcaaa aaaggtctgt gtgatttagc aagtgaaaca
     1321 ccagagttta gcgactggcg aacgaactat gccgtttctg gtgagcggtt gcgattgaat
     1381 accgaacaag ccaccgccgt tggcgcaatt catagcgcgg cagatacttt ttctgcctgg
     1441 ctgctggcgg gcgttaccgg ttccggtaaa acggaggttt atctcagtgt actggaaaac
     1501 gtgctcgctc agggcaaaca ggcgctggtg atggtgccgg aaatcggcct gacaccgcaa
     1561 actatcgccc gttttcgtga acgttttaat gcccccgtgg aagttctgca ttccggcctg
     1621 aacgacagcg agcggctttc ggcgtggttg aaagcgaaaa atggcgaggc ggcgattgtg
     1681 atcggcaccc gctccgcgct gtttacgccg tttaaaaatc tcggcgtgat tgtcattgat
     1741 gaagagcacg acagctccta caagcagcag gaaggctggc gctatcatgc ccgcgacctg
     1801 gcggtgtatc gtgcgcatag cgagcaaatc ccgattattc ttggctccgc aacgcccgcg
     1861 ctggaaacgt tatgcaacgt ccagcagaaa aaataccgcc tgctgcgcct gacccgtcgg
     1921 gcagggaatg cgcgtccggc aattcaacat gtgctggatt taaaaggtca gaaggtgcag
     1981 gcaggtctgg ctccggcgtt aatcacccgt atgcgccagc atttacaggc caacaatcag
     2041 gttatcctct ttcttaatcg tcggggtttt gcgcctgcgc tgctgtgcca cgactgcggc
     2101 tggatagccg aatgcccacg ttgcgatcac tactacacgc tgcatcaggc gcaacagcat
     2161 ttgcgctgtc accactgtga cagtcagcgt ccggtgccgc gccagtgccc ttcctgcggt
     2221 tccacgcacc tggtccccgt ggggctgggc accgaacagc ttgaacagac gcttgcgccg
     2281 atgttccccg gcgtgcccat ttcacgtatc gaccgcgata ccaccagccg caaaggggcg
     2341 ctggaacagc aactggcgga agtgcatcgc ggcggcgcga ggatcttgat tggcacgcaa
     2401 atgctggcga aaggtcacca ctttccggat gtgacgctgg tggcgttact ggatgtggac
     2461 ggcgcgctgt tttctgccga ttttcgctcg gcagaacgtt tcgcccagct ttacacccag
     2521 gtcgccggtc gtgccgggcg tgcgggtaaa cagggcgaag tggtgctgca aacgcaccat
     2581 ccggaacatc ctctgttgca aacgttgctc tataaaggct acgacgcctt tgccgaacag
     2641 gcgctggctg agcgacgcat gatgcagctg ccgccgtgga ccagccatgt gattgtgcgt
     2701 gcggaagatc ataacaatca gcatgcgccg ctgttcctgc aacaactgcg taatctgatc
     2761 ctttccagcc cgctggcaga cgacaaactg tgggttctcg gcccggttcc ggctctggca
     2821 cctaagcgtg gtggccgctg gcgctggcag atcttgttgc agcacccttc ccgcgtgcgc
     2881 ttgcaacaca tcattagtgg tacgctggcg cttattaata caataccgga ttcccgcaag
     2941 gtgaaatggg tgttggatgt cgatcccatc gaaggctaat gcgcaaacgt tgcgaggcgg
     3001 atcgaaaaat tcaatattca tcacactttt catgaaaatt ctgtaaccgt tttcacgcgc
     3061 tatctgctaa aaatgttgcc gatgtgaagt aaacatggat gtagtacgcc tgacgtgcca
     3121 ggcgaggagt gagtgtgaaa gcgaagaagc aggaaactgc cgcgaccatg aaagacgttg
     3181 ccctcaaggc aaaagtctct acagcgactg tctcccgagc attaatgaat cccgataaag
     3241 tctcccaggc cacccgtaat cgggttgaaa aagcggcccg ggaagtgggt tatttaccgc
     3301 agcctatggg gcgcaacgtc aagcgtaatg aatcccgcac cattctggtg attgtcccgg
     3361 atatctgcga tcccttcttt agcgaaatta ttcgcggtat cgaagttacg gcggcaaatc
     3421 acggatatct ggtgctgatt ggcgactgtg cgcatcaaaa tcagcaggaa aaaaccttta
     3481 tcgatttgat catcaccaag caaattgatg gcatgttgct gctgggttca aggctgccgt
     3541 ttgatgccag cattgaggaa cagcgtaatc tgccgccgat ggtgatggcg aacgaattcg
     3601 caccggaact ggagctacct acagttcata tcgacaatct gaccgccgca tttgatgcag
     3661 taaattatct atatgagcaa gggcataaac ggattggctg tatagccggt cccgaagaga
     3721 tgccgctgtg tcactaccgc ctgcaaggtt acgttcaggc gctgcgtcgc tgcggcatta
     3781 tggttgatcc gcaatacatc gcccgtggcg acttcacctt cgaagccgga agcaaagcga
     3841 tgcagcagct gcttgatctt ccacaaccgc ctactgctgt cttctgccat agcgatgtga
     3901 tggcgctcgg cgcactttct caggcaaaac gccaggggct gaaagtcccg gaagaccttt
     3961 ccataatcgg ttttgataac atcgacctga cgcaattttg tgatccgccg ctgacaacca
     4021 tcgcgcagcc gcgttacgaa atcggtcggg aagctatgct gttattgctt gatcaaatgc
     4081 aggggcaaca cgttggcagt ggctctcgtt taatggactg cgaacttatc atccggggat
     4141 caacacgcgc gttaccttaa agtaaacctt taagactgcc gtgactggtc aaaggcccgc
     4201 cgcttaagta acatggcggg ctgacgaacg aataaataca gcgaaacgat agtggcacaa
     4261 cgagattatg tacgccgcag ccaaccggca ccttcgcggc gaaaaaagag cacctcacgg
     4321 aaaaagcaac gaaatctgcc tgcggtttct cccgctatgg tcgctattgc tgccgccgtt
     4381 cttgtgacct ttatcggtgg tctgtacttc attacgcatc acaagaaaga agagtccgag
     4441 acgctgcaaa gccagaaagt gaccggtaac ggactaccac caaaaccaga agaacgctgg
     4501 cgctacatta aagagctgga aagtcgccag ccgggtgtgc gtgcgcccac agaaccttct
     4561 gccggtggtg aagtgaaaac gccggagcaa ctgacaccag aacaacgtca gcttcttgaa
     4621 caaatgcagg ctgatatgcg ccagcagcca acgcagctgg ttgaagttcc gtggaatgaa
     4681 cagacgccgg aacagcgtca gcaaacgcta caacgccagc gtcaggcgca gcaattagcg
     4741 gagcagcaac gcctggctca gcagtcgcgt acgactgaac aaagctggca gcagcagacg
     4801 cgtacgtcgc aagccgcgcc ggtacaggcg caaccgcgcc agtctaaacc ggcttctact
     4861 cagcagccgt accaggatct gctgcaaacc cctgcgcaca cgaccgcgca gtcgaagccg
     4921 cagcaagctg cgccggttgc tcgtgccgct gacgcgccaa aaccgacggc ggagaaaaaa
     4981 gacgaacgcc gctggatggt gcagtgcggt tcgttcagag gcgcggaaca ggcagagacg
     5041 gtacgtgctc agctggcgtt cgaaggattt gactcgaaaa tcaccaccaa caatggctgg
     5101 aatcgtgtgg tcattggccc ggtgaaaggc aaagagaacg cagacagcac cctcaatcgg
     5161 ttgaagatgg cgggtcatac aaactgcatt cggctcgccg ccgggggttg aaaccctcaa
     5221 aatccccccc atctataatt gcattatgcc ccgtactttt gtacggggtt tgtactctgt
     5281 attcgtaacc aaggggtcag ctcgtgacaa ctatagtaag cgtacgccgt aacggccatg
     5341 tggtcatcgc tggtgatggt caggccacgt tgggcaatac cgtaatgaaa ggcaacgtga
     5401 aaaaggtccg ccgtctgtac aacgacaaag tcatcgcggg ctttgcgggc ggtactgcgg
     5461 atgcttttac gctgttcgaa ctgtttgaac gtaaactgga aatgcatcag ggccatctgg
     5521 tcaaagccgc cgttgagcta gcgaaagact ggcgtaccga tcgcatgctg cgcaaacttg
     5581 aagcactgct ggcagtcgcg gatgaaactg catcgcttat catcaccggt aacggtgacg
     5641 tggtgcagcc agaaaacgat cttattgcta tcggctccgg cggcccttac gcccaggctg
     5701 cggcgcgtgc actgttagaa aatactgaac tgagtgctcg tgaaattgct gaaaaggcgt
     5761 tggatattgc aggcgacatt tgcatctata ccaaccattt ccacaccatc gaagaattaa
     5821 gctacaaagc gtaaggatct cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac
     5881 tggataagca catcatcggc caggacaacg ccaagcgttc tgtggcgatt gctctgcgta
     5941 accgctggcg tcgcatgcag ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata
     6001 tcctgatgat cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgt ctggctaagc
     6061 tggcgaatgc gccgttcatc aaagttgaag cgaccaaatt caccgaagta ggctacgtcg
     6121 gtaaggaagt ggattctatt attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg
     6181 tccaggctat cgagaaaaac cgttatcgcg ctgaagaact ggcagaagaa cgtattctcg
     6241 acgtgctgat cccacctgct aaaaacaact ggggacagac cgaacagcag caggaaccgt
     6301 ccgctgctcg tcaggcattc cgcaaaaaac tgcgtgaagg ccagcttgat gacaaagaaa
     6361 tcgagatcga tcttgccgca gcaccgatgg gcgttgaaat tatggctcct ccaggcatgg
     6421 aagagatgac cagccagctg cagtccatgt tccagaacct gggcggccag aagcaaaaag
     6481 cgcgtaagct gaaaatcaaa gacgccatga agctgctgat tgaagaagaa gcggcgaaac
     6541 tggtgaaccc ggaagagctg aagcaagacg ctatcgacgc tgttgagcag cacgggatcg
     6601 tgtttatcga cgaaatcgac aaaatctgta agcgcggcga gtcttccggt ccggatgttt
     6661 ctcgtgaagg cgttcagcgt gacctgctgc cgctggtaga aggttgcacc gtttccacca
     6721 aacacgggat ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg ttccagattg
     6781 cgaaaccgtc tgacctaatc ccggaactgc aaggtcgtct gccaatccgc gttgaactgc
     6841 aggcactgac caccagcgac ttcgagcgta ttctgaccga gccgaatgcc tctatcaccg
     6901 tgcagtacaa agcactgatg gcgactgaag gcgtaaatat cgagtttacc gactccggta
     6961 ttaagcgcat cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac atcggtgctc
     7021 gtcgtttaca cactgttctg gagcgtttaa tggaagagat ttcctacgac gccagcgatt
     7081 taagcggtca aaatatcact attgacgcag attatgtgag caaacatctg gatgcgttgg
     7141 tggcagatga agatctgagc cgttttatcc tataatcgcg ttcaatcatt ttcatcattg
     7201 tttgatgggg ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa caacaaatta
     7261 gccgaactca ggcgtggctg gaaagtttac gacctaaaac cctccccctc gcctttgctg
     7321 caattatcgt cgggacggcg ctggcatggt ggcaaggtca cttcgatccg ctggtcgccc
     7381 tgctggcact aattaccgcc gggctattac agatcctttc taacctcgcc aatgattacg
     7441 gcgatgcggt aaaaggcagc gataaacctg accgcattgg gccgctacgc ggcatgcaaa
     7501 aaggggtcat tacccagcaa gagatgaaac gggcgctcat tattaccgtc gtgctcatct
     7561 gtctctccgg gttggcactg gttgcagtgg catgccatac gctggccgat tttgtcggtt
     7621 tcctgattct tggcgggttg tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc
     7681 cttatggtta tatcggtctg ggtgatattt ccgtactggt tttctttggc tggttgagtg
     7741 tcatggggag ctggtattta caggctcata cattgattcc ggcactgatc cttccggcga
     7801 ccgcatgcgg cctgctggca acggcagtac tgaatattaa taacctgcgt gatatcaata
     7861 gcgaccgcga aaatggcaaa aacacgctgg tggtgcgctt aggtgaagtg aacgcgcgtc
     7921 gttatcatgc ctgcctgctg atgggctcgc tggtgtgtct ggcgctgttt aatctctttt
     7981 cgctgcatag cctgtggggc tggctgttcc tgctggcggc accattactg gtgaagcaag
     8041 cccgttatgt gatgcgggaa atggacccgg tggcgatgcg accaatgctg gaacgtactg
     8101 tcaagggagc gttactgact aacctgctgt ttgttttagg gatattccta agccagtggg
     8161 cagcataact gacaaatatc aattaacaat tgatgatttt gccaacagcc cacatagcgc
     8221 gatatactga aaattctcgc agcaactgaa tgttaagcct atgaaatacg atacttccga
     8281 gctttgtgac atctatcaag aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg
     8341 cggacgggcg tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg acaacgggtt
     8401 gctgtacgat ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg atggcggtgg
     8461 ttctgttcgt cgcgcactgg tcgatgctga actggcgcgt ctggcagtac aaaatgaatg
     8521 ggaaggtctg gtcatttacg gcgcggtgcg tcaggtagat gacctggaag agttggatat
     8581 cggcatccag gcgatggcgg caattccggt tggtgccgct ggcgaaggca ttggcgaaag
     8641 cgatgtccgc gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc tttatgccga
     8701 caataccggg attattcttt ctgaagatcc gctggatatt gaatgataga aaaggcaccg
     8761 caaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt acgcggaaga
     8821 tgaagcgtaa tcagacctct tccatgcgac ccagcagggc ctgcagacgt tcctgccagc
     8881 cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc tgatgctggg
     8941 cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg atttccatct
     9001 gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc tcaaacactt
     9061 ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa aatcctcgtc
     9121 ccgattaccg gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga
     9181 agccgcgcca tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat
     9241 ccttctcctg gtgacataat ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat
     9301 gatctaacaa acatgcatca tgtacaatca gatggaataa atggcgcgat aacgctcatt
     9361 ttatgacgaa gcacacacat tttaagttcg atatttctcg cttttgctcg ttaacgataa
     9421 gtttacagca tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat
     9481 taactcttca ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg
     9541 aattcctcgg taccgggttg ttgattttct tcggtgtggg ttgcgttgca gcactaaaag
     9601 tcgctggtgc gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa
     9661 tggccatcta cctgaccgca ggggtttccg gcgcgcatct taatcccgct gttaccattg
     9721 cattgtggct gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag
     9781 ttgccggcgc tttctgtgct gcggctttag tttacgggct ttactacaat ttatttttcg
     9841 acttcgagca gactcatcac attgttcgcg gcagcgttga aagtgttgat ctggctggca
     9901 ctttctctac ttaccctaat cctcatatca attttgtgca ggctttcgca gttgagatgg
     9961 tgattaccgc tattctgatg gggctgatcc tggcgttaac ggatgatggc aacggtgtac
    10021 cacgcggccc tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta
    10081 tgggcccatt gacaggtttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg
    10141 cctggctggc gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc
    10201 tggtgccgct tttcggccct atcgttggcg cgattgtagg tgcatttgcc taccgcaaac
    10261 tgattggtcg ccatttgcct tgcgatatct gtgttgtgga agaaaaggaa accacaactc
    10321 cttcagaaca aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa
    10381 aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac
    10441 gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggt
    10501 tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg
    10561 ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt
    10621 gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg
    10681 cagtgccgtc gtaccgcaga aatctgcgag catttaaaac gtgacggttt agaagattat
    10741 atccgcagca ataccggtct ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg
    10801 atcctcgacc atgtggaagg ctctcgcgag cgtgcacgtc gtggtgaatt gctgtttggt
    10861 acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac
    10921 accaacgcct ctcgtaccat gttgttcaac atccataccc tggactggga cgacaaaatg
    10981 ctggaagtgc tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagta
    11041 tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc
    11101 ggtgaccagc aggccgcgct gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac
    11161 acctatggca ctggctgctt tatgctgatg aacactggcg agaaagcggt gaaatcagaa
    11221 aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa
    11281 ggtgcggtgt ttatggcagg cgcatccatt cagtggctgc gcgatgagat gaagttgatt
    11341 aacgacgcct acgattccga atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat
    11401 gtggttccgg catttaccgg gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg
    11461 attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct
    11521 attgcttatc agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg
    11581 cacgccctgc gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca gttccagtcc
    11641 gatattctcg gcacccgcgt tgagcgcccg gaagtgcgcg aagtcaccgc attgggtgcg
    11701 gcctatctcg caggcctggc ggttggcttc tggcagaacc tcgacgagct gcaagagaaa
    11761 gcggtgattg agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac
    11821 gcaggctgga aaaaagcggt taaacgcgcg atggcgtggg aagaacacga cgaataatgt
    11881 aaatgccgaa tgaagcgttt atgccgcatc cggtagtccc gaaacgtgcg ggggcaaccc
    11941 cgcacacatc aataatccct cccttcccct gtgctacact tcgcgccatt ccttactgct
    12001 tagagtttgc tatgagacga gaacttgcca tcgaattttc ccgcgtcacc gaatcagcgg
    12061 cgctggctgg ctacaaatgg ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg
    12121 taaacgccat gcgtattatg ctcaaccagg tcaacattga cggcaccatc gtcattggtg
    12181 aaggtgaaat cgacgaagca ccgatgctct acattggtga aaaagtcggt actggtcgcg
    12241 gcgacgcggt agatattgct gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc
    12301 aggctaacgc gctggcggtg ctggcagtag gcgataaagg ctgcttcctc aatgcgccgg
    12361 atatgtatat ggagaagctg attgtcgggc cgggagccaa aggcaccatt gatctgaacc
    12421 tgccgctggc ggataacctg cgcaatgtag cggcggcgct cggcaaaccg ttgagcgaac
    12481 tgacggtaac gattctggct aaaccacgcc acgatgccgt tatcgctgaa atgcagcaac
    12541 tcggcgtacg cgtatttgct attccggatg gcgacgttgc ggcctcaatt ctcacctgta
    12601 tgccagacag cgaagttgac gtgctgtacg gtattggtgg cgcgccggaa ggcgtagttt
    12661 ctgcggcggt gatccgcgca ttagatggcg acatgaacgg tcgtctgctg gcgcgtcatg
    12721 acgtcaaagg cgacaacgaa gagaatcgtc gcattggcga gcaggagctg gcacgctgca
    12781 aagcgatggg catcgaagcc ggtaaagtat tgcgcctggg cgatatggcg cgcagcgata
    12841 acgtcatctt ctctgccacc ggtattacca aaggcgatct gctggaaggc attagccgca
    12901 aaggcaatat cgcgactacc gaaacgctgc tgatccgcgg caagtcacgc accattcgcc
    12961 gcattcagtc catccactat ctggatcgca aagacccgga aatgcaggtg cacatcctct
    13021 gattgatttg atcgattgag ccttccagtc cttcgggact ggaatttttt tgttcggaga
    13081 acgaagataa ggcaagtcaa tcaaaacagg agaaaaacat ggctgattgg gtaacaggca
    13141 aagtcactaa agtgcagaac tggaccgacg ccctgtttag tctcaccgtt cacgcccccg
    13201 tgcttccgtt taccgccggg caattcacca agcttggcct tgaaatcgac ggcgaacgcg
    13261 tccagcgcgc ctactcctat gttaactcgc ccgataatcc cgatctggag ttttacctgg
    13321 tcaccgtccc cgatggcaaa ttaagcccac gattggcggc actgaaacca ggcgatgaag
    13381 tgcaggtggt tagcgaagca gctggcttct ttgttctgga tgaagtaccg gactgcgaaa
    13441 cgctatggat gctggcaacc ggtacagcga ttggccctta tttatcgatt ctgcaactag
    13501 gcaaagattt agatcgcttc aaaaatctgg tcctggttca cgccgcacgt tatgccgccg
    13561 acttaagcta tttgccactg atgcaagaac tggaaaaacg ctacgaaggg aaactgcgca
    13621 ttcagacggt ggtcagtcgg gaaacggcag cggggtcgct caccgggcgg ataccggcgt
    13681 taattgaaag tggggaactg gaaagcgcga ttggcctgcc gatgaataaa gaaaccagcc
    13741 atgtgatgct gtgcggcaat ccacagatgg tgcgcgatac acaacagttg ctgaaagaga
    13801 cccggcagat gacgaaacat ttacgtcgcc gaccgggcca tatgacagcg gagcattact
    13861 ggtaagcggt tacttatcga taaacggcac gatgagcaaa tccgcactca tcttattgat
    13921 catcccgcga tatgccggca tcaaacggtt gataaatgag tgatgatgac cacagacaag
    13981 gaggtcgcac tgctcttttt gcataatttc cagcagtgtt tccggcattt ctccgcgttc
    14041 aatacgcagt tttgtcttcg gccattgaat atttttcgtc agtttataca gcttgttatc
    14101 cgacttattc ttcaacaatt gaagaatatc ttctgttgca gggaagtaga tacccggata
    14161 caactcgctt aagccatcat caatatgaat taacgtcagg tgagcgtcat tatgtctggc
    14221 gagctccaga gctttattca ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc
    14281 aatgtgttta taagccatgt ttaactcctt ctaaagcaac tccatcaagc taacgaacgc
    14341 agtgataact caaaaataat catctcggcc tggcatgaga aagtgaaggc cgcatcaagc
    14401 tcaacttgcc cctccagatc cttaatcttg taggccacgc tggccccttc atctgcagag
    14461 gatgcagcgg cggcaagctc tttcagtttg gtcataaaag actctgcttc ttcgcgggtg
    14521 gcaaaaaagc gcgaaaactt actggtacag ttgtcgttat caatcaccgt accgatatca
    14581 atcgcacatc ctttagtact gcatttatct acgacatctt tcatagggca cctctgtata
    14641 ttcgcctctc tgtcgattac gacttaagaa taatgcgccg ttaagggaag aacaaacaag
    14701 taatgatctt atggattacc attttttttg atattgctta aggtgaagag ttcagaagga
    14761 ttttccgggt attgtcattt gcatcgtgga aataacaatc ccggaaaaaa atagcagtaa
    14821 gagatatatt aaaggttaat tacttgattt attgtcggct ttatacttca catcctgagt
    14881 atctttacca tatttatttt cgccttgtgt gccaacaaac gcaccaagat cgataagcat
    14941 catcaccaga atcaacgtcg ggacaaaacg ccccaccgcc cattgccaga cacccggtaa
    15001 aatcgcccag ttacccgcca gcagcatcca cgccacaatc atcagaaatg cccatgcgcc
    15061 ggaacgcccg cgatcatgca agcgcttaac agttactgcc gctgttggcc aaagcaagca
    15121 cacaaggcaa aacgccgcgg tctgaatatc gagtaaattt ttacccgcca gtgagaaaag
    15181 caccagcatg cctgcgaacc acaggcctat ccaaatccag aaatcacggc gtccaatacg
    15241 ccctttaaat gagaataacc attgctgtat ggtcatgtaa gttccttgat ggttgtcttt
    15301 tccaggattc tacccttttg acaagggcga cagatatcgt tttaatcgga gccagtcata
    15361 acaaaaggta ctgtcaatga agccagggtg tacgctgttt tttctcttat gttctgcatt
    15421 aaccgttaca acaacggcgc atgcacaaac accagatacg gcaacgaccg cgccttatct
    15481 gctggctgga gcccctactt tcgatctctc catcagccag tttcgagaag actttaacag
    15541 ccagaatcct agcctgccac tgaacgaatt tcgtgccatc gacagcagtc ccgacaaagc
    15601 caatctcact cgtgctgcaa gtaaaattaa cgagaacttg tatgcttcta cagcgctgga
    15661 gcgcggtacc ttaaaaatca aaagcattca aatgacctgg ctacccatcc aggggccaga
    15721 gcaaaaagca gcgaaagcga aagctcagga atacatggca gcagtgatcc gcacactcac
    15781 cccattaatg accaaaacac aaagccagaa aaaactgcag tcgctactaa cggcggggaa
    15841 aaacaaacgt tattacaccg agacagaagg tgcactgcgt tatgttgtcg cggacaacgg
    15901 cgaaaagggg ctgaccttcg ctgttgaacc gattaagctg gcgctatctg aatcgcttga
    15961 aggtttgaat aaatgacaaa aagcaaagcc tttgtgccga tgaatctcta tactgtttca
    16021 cagacctgct gccctgcggg gcggccatct tcctttattc gcttataagc gtggagaatt
    16081 aaaatgcgac atcctttagt gatgggtaac tggaaactga acggcagccg ccacatggtt
    16141 cacgagctgg tttctaacct gcgtaaagag ctggcaggtg ttgctggctg tgcggttgca
    16201 atcgcaccac cggaaatgta catcgatatg gcgaagcgcg aagctgaagg cagccacatc
    16261 atgctgggtg cgcaaaacgt ggacctgaac ctgtccggcg cattcaccgg tgaaacttct
    16321 gccgctatgc tgaaagacat cggcgctcag tacatcatca tcggccactc tgaacgtcgt
    16381 acttaccaca aagagtctga cgaactgatt gcgaaaaaat tcgcggtgct gaaagagcag
    16441 ggcctgactc cggttctgtg catcggtgaa accgaagctg aaaacgaagc gggcaaaact
    16501 gaagaagttt gcgcacgtca gatcgacgcg gtactgaaaa ctcagggtgc tgcggcattc
    16561 gaaggtgcgg ttatcgctta cgaacctgta tgggcaatcg gtactggcaa atctgcaact
    16621 ccggctcagg cacaggctgt tcacaaattc atccgtgacc acatcgctaa agttgacgct
    16681 aacatcgctg aacaagtgat cattcagtac ggcggctctg taaacgcgtc taacgctgca
    16741 gaactgtttg ctcagccaga tatcgacggc gcgctggttg gcggtgcttc tctgaaagct
    16801 gacgctttcg cagtgatcgt taaagctgca gaagcggcta aacaggctta agtctgacag
    16861 gtgccggatt tcatatccgg cacttacttt ccttaactcc tcgccttaac gcaaaatctc
    16921 acactcatga tcctggattt cctcggctga agcacggttc agcgtcagta gatttcgttg
    16981 tgtcgccagc aacacaaacg aattatcact ctgccgtacc atcgccagcc cgtagcttcc
    17041 catatgttcc cgcgcctcag gcacttcttc tgcaagcatc ataaatgggc tgcgttgtac
    17101 cagttcgctt tccgttaccc gacgcgccag gtattcatgc ccgcgcaaac cacctggcag
    17161 tggcaaccag cggctgctga tgttcgccag atttttatcc agttgttcgc gcacatcagg
    17221 acgaatacaa gagatatgaa tatgaaaatg gttttgcgta cgcccggtgc gggagttgat
    17281 cgccaaagaa accgcgcgat cgggaaccgg ctggccgtat tttttgctca taaaatcacg
    17341 cgcctgccag gccaaccaaa agaagttcgg cgttgaagga tcggtcaaca aaggactttc
    17401 agtaccgtta atacgatacg ttggcatcaa cagatattgc agtggaccat taagatcttt
    17461 taaaaccacg tatccggcat tgggtttgac ttccgcacat ggcgaaggat tttgattttg
    17521 ctgctgattg ggcaaacatt cctcaaggac aatcttacgt aatgtatccg actcttcacc
    17581 ggttaatttc cagtaaccaa taccggcagc cacaacggcg ataactatca tcaccaaaaa
    17641 aagaagaccc gcttttttca tctttttttc cctgtacctc aaagagatgc aagggtaacg
    17701 caaaatcgtg acaaataaaa aacccggtcg cgagaaacca ccgggtttga taattatcct
    17761 ggggaaatca gcgtttgctg atctgatcga acgtaccgcc gttagcaaaa tgctcttttt
    17821 gcgctttcgt ccagccgccg aactcttcat caatggtgaa taacttcagc tttggaaacg
    17881 cattttcgta ctttttcgct acttcagcgt cgcgcggacg gtagtagttt ttcgcggcaa
    17941 tttcctgacc ttctggcgag tagagatatt tcaggtaggc ttccgccacc tctttggtgc
    18001 cttttttctc gaccacttta tcgaccaccg acacggttgg ctctgcgagg atagactcac
    18061 tcggcgtgac gatttcgaat ttatctttcc ccagttcatt cgctaccagc agagcttcgt
    18121 tttcccaggc gatcagtaca tcgccaatcc cgcgctcgac aaaggtgtta gtggagccgc
    18181 gcgcgccaga atccagaact tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt
    18241 ttgcctgatc gttgttgttg tgatgcagcg cgtagcccca ggcagccagg tagttccagc
    18301 gtgcgccacc agagcttttc ggattaggcg tgatcaccga aacacccggt ttaatcagat
    18361 cgttccagtc atggatctgc ttcggattgc ccttacgcac caggaaaaca atggtggaag
    18421 tgtacggtgc ggagttatcc ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt
    18481 ccgcaatcgc gtccacgtca taggccagag ccagcgtgac aacatcagct tcaataccgt
    18541 tgattaccga cgtcgcttgt ttacctgaac caccgtgtga ctgacgaatc accacgttat
    18601 caccagtttg ctgtttccag tgggcgctga atgccttgtt gtactgttcg tacaattcgc
    18661 gcgttggatc atatgaaacg ttaagaagct gaatatcctt tgccataacg ttggttgccg
    18721 ccagcaaaaa tgttaaccct acgccccact tgttcatcgc ccgactctct tatgttgtgt
    18781 tgtgatgagc aaagcgtgcc agaaggttaa ccaaacatta aagaataaaa aaagattggc
    18841 tataacttgc gggtatatgt tgggggttaa aaaggcggaa gaaatccgcc tcatattgct
    18901 gacaaagtgc gctttgtcca tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag
    18961 tttgcaaatt caataaattg cagaattcat gtaggcctga taagcgaagc gcatcaggca
    19021 tttttgcttc tgtcatcggt ttcagggtaa aggaatctgc ctttttccga aatcattaat
    19081 acagtttttt cgcgcagtcc agccagtcac ctttgaacgg acgcttcatg ttttcgatag
    19141 cgtcgatgat gtcgtggtga accagctgtt cgttctggat acctacacaa cgaccgccgt
    19201 aacctgccag cagcagatcg atagcgtaag cgcccatacg ggaagccaga atacggtcgt
    19261 aaggcaccgg agaaccaccg cgctggatgt ggcccagcac agttgcgcgg gtttcacgac
    19321 cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg
    19381 ccacgatcgc gtgtttttta cctttcgcga tacccgcttt gatttcgttt accaggtctt
    19441 cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca gccaccggca atggccgcag
    19501 ccaacgtcag atcgccacaa taacggccca tcacttccac cacggaaata cgctggtgag
    19561 aagaagaggt gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga
    19621 aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc agaccgatgc
    19681 acgggaagcc catttcggtc agacgcattg cacccatgta ggaaccgtca ccgccgataa
    19741 ccaccagcgc gtcgatccca cgttttttca ggttttcgat agccacggcg cggatgttct
    19801 cgtcgcggaa ttccgggaag cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt
    19861 cagaaacgct gtaacggtct agctgtacca tacggtcttc atacagaccc agatagccgt
    19921 cataaatgcc cattacttcc agaccttctg tcagcgcaga acgaacaacc ccgcgaattg
    19981 cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac accgattttc ttaatcatga
    20041 ctacctctga actttggaat gcaaaataaa atctgttgcc ggaagtcttc ttgcacatcg
    20101 aagtgatcca acgaatgtgc aaatagtata acaatcactt cctgctgaat tgattcaggt
    20161 caggccaaat ggcggtattt tatacacaaa atgcgggtct ggctctcttt tatactgatt
    20221 atgaaagcat agaccgttta ccctccctgg gtacgacgga acaggggtcc tgatggataa
    20281 ttacatccga tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc gccaccatat
    20341 gtgcctgaac caaaggcaga gagtcttcca tttccaaatg aatctgaata aagcgggtcg
    20401 gccctgactg ccgcgtgcga agatcgtgag cgccgctaac acccggccag gaagtcacga
    20461 tatcaataat ttcttgccgt tcctcatcag gcaatgcgcg atccagtaat gactgtaccg
    20521 cctcatatcc catgcgtaac gcgctatata aaatatagat gccgattccc aatgcaaaca
    20581 gagcatcggc gcgatgccag ccgtaccagg acaaccccag cgccagcaga attgcgccgt
    20641 tcatcataac atcagactgg taatgtagca tatcagcccg caccgcctgg ctttgcgtcc
    20701 ggcgcaccac ccaacgctga aacgagacaa ggataatcgt acaaattagc gccacaattg
    20761 tcacgataac cccgacgcct ggatctgtca tcggtgttgg agatatcaga tgttgaatac
    20821 ccgtcaaaaa caggaatagt gccgaaccgg agataaacat actttgcgcc agcgccgcga
    20881 gggactctgc tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc agggaatatc
    20941 gcaccaccag taaattcgtc aacgacgcgc cgatatccac cagcgaatcc accagcgcgg
    21001 cgagaatact caccgacccg gtataccacc atgcaaaaat tttaatcagc aatagcaacg
    21061 aagccatcgc cgtcgcagca atcgccgccc gactgaccag ccgtccataa gattgattca
    21121 taaatactcc cgctatcaac tgacgctagt ataacggaag caaatcatct gcaatgcatt
    21181 aagcagcagg caaattgagg ataaaaaaaa cccccacatc atgtggggga agacagggat
    21241 ggtgtctatg gcaaggaaaa cagggtttac tactgggaac gtgagttgct actactcaat
    21301 agcttcaacg atgaactttt ttgccattgc gtcacgtcac gcaactgctc cattcgttgt
    21361 tgatgtttct cgtttaaaac cgcttgctgc tccggcgtta acaggcgata catttggttg
    21421 cggacttttg ccatctcaac ctgacgagca atttgctcat tcgccatttt ttctgcctgt
    21481 gcgcgcacag cgttttcatc aaaattttct gcggtgacaa ggcgatgcat tgtctccagt
    21541 tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt gcataagatc tcgcatctgc
    21601 tgacgctgat gttcggttaa acttatgccg tcgaacatat ggctctgcgt actgcgctgc
    21661 gtaagttctt cacccggatg ccagttatcg cctgaaccga cttcagcagc gtggcttaat
    21721 gaactgactg ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat catttgctcc
    21781 caaaatcttt ctgtcgcgat tcaacgatag agagtttacg attcaggctg caaacatgcg
    21841 tcagggggtg taaaacaacg taaagtcatg gattagcgac gtctgatgac gtaatttctg
    21901 cctcggaggt atttaaacaa tgaataaaat cctgttagtt gatgatgacc gagagctgac
    21961 ttccctatta aaggagctgc tcgagatgga aggcttcaac gtgattgttg cccacgatgg
    22021 ggaacaggcg cttgatcttc tggacgacag cattgattta cttttgcttg acgtaatgat
    22081 gccgaagaaa aatggtatcg acacattaaa agcacttcgc cagacacacc agacgcctgt
    22141 cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg agctgggcgc
    22201 agatgactat ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta ttcgcgcgat
    22261 cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt caccgacact
    22321 ggaagttgat gccttagtgc tgaatccagg ccgtcaggaa gccagcttcg acgggcaaac
    22381 gctggagtta accggtactg agtttaccct gctctatttg ctggcacagc atctgggtca
    22441 ggtggtttcc cgtgaacatt taagccagga agtgttgggc aaacgcctga cgcctttcga
    22501 ccgcgctatt gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc gtaaagatgg
    22561 tcacccgtgg tttaaaacct tgcgtggtcg cggctatctg atggtttctg cttcatgata
    22621 ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga cgctggcgct ggtgttgatg
    22681 ttggttttga tgttacccaa gctcgattca cgccagatga ccgagcttct ggatagcgaa
    22741 cagcgtcagg ggctgatgat tgagcagcat gtcgaagcgg agctggcgaa cgatccgccc
    22801 aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc accgccagga
    22861 cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg cagcgaaatg
    22921 cagatcattc gtaactttat tggtcaggcc gataacgccg atcatccgca gaagaaaaag
    22981 tatggccgcg tggaactggt cggtccgttc tccgtgcgtg atggcgaaga taattaccaa
    23041 ctttatctga ttcgtccggc cagcagttct caatccgatt tcattaactt actgtttgac
    23101 cgcccgctat tactgctgat tgtcaccatg ttggtcagta cgccgctgct gttgtggttg
    23161 gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga agttgcccag
    23221 ggaaacttac gccagcaccc ggaactggaa gcggggccac aggaattcct tgccgcaggt
    23281 gccagtttta accagatggt caccgcgctg gagcgcatga tgacctctca gcagcgtctg
    23341 ctttctgata tctctcacga gctgcgcacc ccgctgacgc gtctgcaact gggtacggcg
    23401 ttactgcgcc gtcgtagcgg tgaaagcaag gaactcgagc gtattgaaac cgaagcgcaa
    23461 cgtctggaca gcatgatcaa cgatctgttg gtgatgtcac gtaatcagca aaaaaacgcg
    23521 ctggttagcg aaaccatcaa agccaaccag ttgtggagtg aagtgctgga taacgcggcg
    23581 ttcgaagccg agcaaatggg caagtcgttg acagttaact tcccgcctgg gccgtggccg
    23641 ctgtacggca atccgaacgc cctggaaagt gcgctggaaa acattgttcg taatgctctg
    23701 cgttattccc atacgaagat tgaagtgggc tttgcggtag ataaagacgg tatcaccatt
    23761 acggtggacg acgatggtcc tggcgttagc ccggaagatc gcgaacagat tttccgtccg
    23821 ttctatcgga ccgatgaagc acgcgatcgt gaatctggcg gtacaggatt ggggctggcg
    23881 attgttgaaa ccgccattca gcagcatcgt ggctgggtga aggcagaaga cagcccgctg
    23941 ggcggtttac ggctggtgat ttggttgccg ctgtataagc ggagttaaac tccgcatttg
    24001 taggcaggat aaggcgttta cgccgcatcc ggcatttgag caggataagg cgtttacgcc
    24061 gcatccggca tttgagcagg atgcctgatg cgacgctgat agcgtcttat caggcctaca
    24121 ctccgggggt tattttcccc acaaccggcg ggaaaaatct tcgatcttgc cgctcagtcg
    24181 gcgcttttgc atcgtccgcg tccagctttt cgataacccg gcggcggaga gtaaacggtg
    24241 atactgatcg tcatcaaacg gcatatgcca tgcgatggcg gcagcctcct gcacggtcac
    24301 atcgctgaca cgggaaacca actccagcgg cgcgtccgca gacactttcc ctggcgctat
    24361 cacgctatac aaccacccca ccttacctgt gttttgcatc aactgcgcta tatcgctgat
    24421 atcaaaatgg taattgagtt tatagcaagg cgagcgcggc tggctgacct gaattaatgc
    24481 ctctccccag cggaaaatat cgcccatatg aacattactt tccgtgagac cgtcggttga
    24541 gagattttcg ccaaacgcag gcgcaacaaa caactccgcc tgttccggaa attcccgcac
    24601 ccagtagaga tagtgctcac gaggataatg acacagcgct ctgtctggcc cgccgtgaac
    24661 ctttttctcc gcctgctcgt caccttccag ccccagctcc gtcagcatca actcaccatc
    24721 aacctggatt ttggcaattg cgctgggttt gccttcggga taagcctgaa tcttgcctgt
    24781 gtatacatca accggatatc gcatctgttc cctctccacg agaaatttcc ccattagacc
    24841 atatttattt tatgtcaggc gtgctgtttt cgcggtaagt atccaggcag gaactcaata
    24901 gcaaaccgga cgaacgttag ttcatccggc tgtggggatt aaggaagcag gtttatttca
    24961 ccgtacctaa aatttcgatt ttcgctgctc tggctttgac tttacgtgac cagatagagg
    25021 taagaattgg caccagaatc gaagtcacaa tgaccgccgt cgctaccagc gaagttgctg
    25081 ccggagccat cggtttaaac gcaggcacca tttctgcaat cagcacaggt gtcgctaccg
    25141 cggcccctgc ggaactggaa gcggcaattc cggcagtgcc atcgccaccg ccaatcaatt
    25201 tatcggcgat aatcaacgga ataccggtca cgataattac tgccacaccc aacaggatcc
    25261 ccagcaaacc agtctgggca atcacagtca aatcaatggt attgcccagc gcgaaggcaa
    25321 agaatggaat cagcgtttgc accgctttgc tgaaaaattc tcgtaattca gggtcaaggt
    25381 tccccagggc aaagcccacc aggaacggta atactgcgcc gacgaaaaca tgcggttcaa
    25441 acgaggcaat ccctgcagtg cccagaataa tcatcgtcat gagcggcccg gactccaacg
    25501 acatcaacac aaatgcccca gcttcttctt ttgtgccgta ctgctgcatg atggaagcgt
    25561 aaagtccgcc attggtcata tccatcgccg ccaccagcgc cagcgttgaa agtccggcaa
    25621 agaatccaac ttcaacacca tgttccggaa taatgcgcga ggcaattgcc gcaaccaccc
    25681 acgcgacggc aatttttgtt accaccagcg taccggattt acgcaatacc gttcccgtcg
    25741 cgcttaattt tattgacgcc cccatgcaaa aaaaccacac cgccagaatg ggcaccgtac
    25801 cggtaatcat cccgttggtg aatgatccaa aatatttccc cgcccccggc gagaaggtgt
    25861 ggcacagtgc gccaaggaat agcgggacga gcatcatccc ccccgggatt ttctcaatcg
    25921 agcgttttat ctgcatttcc atcacctgtc ggcatatttt taagggctga tgcgtcattc
    25981 ctcaatggag catcagtgtt gatggaaacg aagatagaca ttcatttaat caataaaagt
    26041 gatcaaaacc acatcaattg aaacgctgtt ttatttttat cggatcattg tttttatttt
    26101 gaaattgatc acaaaaaaac caccgccgtt ggcgatggtt cattttttaa gctgatatgc
    26161 ggcctatacg cctcattgca gcaggcggca aatgattatt ttttcgccgc aaaacgtgcc
    26221 gctgcttcgt cccagttcac cacgttccag aactctttaa tgtagtccgg acggcggttc
    26281 tggaatttca ggtagtaagc atgttcccac acatccaggc ccagaatcgg gaagccggaa
    26341 gcgccagaaa tagcttcacc catcagcgga gaatcctggt tagcagtaga aaccaccgcc
    26401 agtttatcgc ctttcagcac cagccatgcc cagccggaac caaagcggga agctgccgct
    26461 ttttcaaatt ctgctttgaa gttatcaacg gagccgaagt cacgttcgat agccgctttc
    26521 aggtcaccct gcagggtggt gccttttttc agacctttcc agaacaggct gtggttagcg
    26581 tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg ctggcagctg gtccagtttg
    26641 gtgatcagct cttcaaccgg caggttggca aattctggca ggctttccag cgccgcgttg
    26701 gcgttgttta cgtaggtctg atggtgtttg gtgtggtgga tttccatggt ctgcttatcg
    26761 aagtgcggtt ccagggcatc gtaagcatac ggcagggatg gcagggtata gctcatattc
    26821 atctccagta ttgtcgggcg gccgattgtt aatgccgcgt aagcagttgg ttcattatag
    26881 ttaattaaat gatattgaaa atgattatca atgccgtact tttcgtaagg gtatggtttt
    26941 gcaggaaaat gcccgagatg tgaagcaaat cacccactta atgccgtgat tgccagtaaa
    27001 tcgacaacgg cggcaacagg cgaaaggtta atcgacagca cgatttttac actcatctcg
    27061 tcggagatgt gaagcgacga aaaatgatga ggataagaag atgagtaacg cgattacgat
    27121 ggggatattt tggcatttga tcggcgcggc cagtgcagcc tgtttttacg ctccgttcaa
    27181 aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtc ggtgggattg tttcgtggat
    27241 tattctgccg tgggccatca gcgccctgtt actaccggat ttctgggcgt attacagctc
    27301 gtttagtctc tctacgctgc tgcctgtttt tctgttcggc gctatgtggg ggatcggtaa
    27361 tatcaactac ggcctgacca tgcgttatct cggcatgtcg atgggaattg gcatcgccat
    27421 tggcattacg ttgattgtcg gtacactgat gacgccaatt atcaacggca atttcgatgt
    27481 gctgattaac accgaaggcg gacgcatgac gttgctcggc gttctggtgg cgctgattgg
    27541 cgtagggatt gtaactcgag ccgggcagtt gaaagagcgc aagatgggca ttaaagccga
    27601 agagttcaat ctgaaaaaag ggctggtgct ggcggttatg tgcggcattt tctctgccgg
    27661 gatgtccttt gcgatgaacg ccgcaaaacc gatgcatgaa gccgctgccg cacttggcgt
    27721 cgatccactg tatgtcgctc tgccaagcta tgttgtcatc atgggcggcg gcgcgatcat
    27781 taacctcggt ttctgtttta ttcgtctgga aaaagtgaag gatttgtcgc taaaagccga
    27841 cttctcgctg gcaaaaccgc tgatcattca caatgtgtta ctctcggcac tgggcgggtt
    27901 gatgtggtat ctgcaattct ttttctatgc ctggggccac gcccgcattc cggcgcagta
    27961 tgactacatc agttggatgc tgcatatgag tttctatgta ttgtgcggcg gtatcgtcgg
    28021 actggtgctg aaagagtgga gcaatgcagg acgccgtccg gtaacggtgt tgagcctcgg
    28081 ttgtgtggtg attattgtcg ccgctaacat cgtcggcatc ggcatggcga attaatcttt
    28141 ctgcgaattg agatgacgcc actggctggg cgtcatcccg gtttcccggg taaacaccac
    28201 cgaaaaatag ttactatctt caaagccaca ttcggtcgaa atatcactga ttaacaggcg
    28261 gctatgctgg agaagatatt gcgcatgaca cactctgacc tgtcgcagat attgattgat
    28321 ggtcattcca gtctgctggc gaaattgctg acgcaaaacg cgctcactgc acgatgcctc
    28381 atcacaaaat ttatccagcg caaagggact tttcaggcta gccgccagcc gggtaatcag
    28441 cttatccagc aacgtttcgc tggatgttgg cggcaacgaa tcactggtgt aacgatggcg
    28501 attcagcaac atcgccaact gcccgaacag caactctgcc atttcgttag caaacggcac
    28561 atgctgacta ctttcatgct caagctgacc gataacctgc cgcgcctgcg ccatccccac
    28621 gctacctaag cgccagtgtg gttgccctgc gctggcgcta aatcccggaa tcgccccctg
    28681 ccagtcaaga ttcagcttca gccgctccgg gcaataaata atattctgca aaaccagatc
    28741 gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg taaaagagat cgccacgggt
    28801 aatgcgataa gggcgatcgt tgagtacatg caggccatta ccgcgccaga caatcaccag
    28861 ctcacaaaaa tcatgtgtat gttcagcaaa gacatgttgc ggataacggt cagccacagc
    28921 gactgcctgc tggtcgctgg caaaaaaatc atctttgaga agttttaact gatgcgccac
    28981 cgtggctacc tcggccagag aacgaagttg attattcgca atatggcgta caaatacgtt
    29041 gagaagattc gcgttattgc agaaaaccat cccgtccctg acgaatatca cgcggtgacc
    29101 agttaaactc tcggcgaaaa agcgtcgaaa agtggttact gtcgctgaat ccgcaatgat
    29161 aggcgatgtc agtaacgctg gcctcactgt ggcgtagcag atgtcgggcc ttcatcagtc
    29221 gcaggcggtt caggtaacgc tgaggcgtca gtcccgtttg ctgcttaagc tgccgatgta
    29281 gcgttcgcag tgaaagagaa aattgatccg ccacggcatc ccaattcacc tcatcggcaa
    29341 aatggtcctc cagccaggcc agaagcaagt tgagacgtga tgcgctgttt tccaggttct
    29401 tctgcaaact gcttttacgc agcaagagca gtaattgcat aaacaaagat ctcgcgactg
    29461 gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc
    29521 acctgctgca atacgctgtg gttaacgcgc cagtgagacg gatactgccc atccagctct
    29581 tgtggcagca actgattcag cccggcgaga aactgaaatc gatccggcga gcgatacagc
    29641 acattggtca gacacagatt atcggtatgt tcatacagat gccgatcatg atcgcgtacg
    29701 aaacagaccg tgccaccggt gatggtatag ggctgcccat taaacacatg aatacccgtg
    29761 ccatgttcga caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa atccgcctga
    29821 gggagccggg gttctatcgc cacggacgcg ttaccagacg gaaaaaaatc cacactatgt
    29881 aatacggtca tactggcctc ctgatgtcgt caacacggcg aaatagtaat cacgaggtca
    29941 ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac
    30001 agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc acaatgaagc aaattgtgaa
    30061 catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa
    30121 ggtcacgaat tcaggcgctt tttagactgg tcgtaatgaa attcagcagg atcacattat
    30181 gacctttcgc aattgtgtcg ccgtcaatct cggcgcatcc agtgggcgcg tgatgctggc
    30241 gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt ttaaaaatgg
    30301 gctgcatagc cagaacggtt atgtcacctg ggatgtggat agcctggaaa gtgccattcg
    30361 ccttggctta aacaaagtgt gtgaggaagg gattcgtatc gatagcattg ggattgatac
    30421 ctggggcgtg gactttgtgc tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc
    30481 ttatcgcgat agccgcacca atggcctaat ggcgcaggca cagcaacaac tcggcaaacg
    30541 cgatatttat caacgtagcg gcatccagtt tctgcccttc aatacgcttt atcagttgcg
    30601 tgcgctgacg gagcaacaac ctgaacttat tccacacatt gctcacgctc tgctgatgcc
    30661 ggattacttc agttatcgcc tgaccggcaa gatgaactgg gagtacacca atgccaccac
    30721 cacacaactg gtcaatatca atagcgacga ctgggacgag tcgctactgg cgtggagcgg
    30781 ggccaacaaa gcctggtttg gtcgcccgac gcatccgggt aatgtcattg ggcactggat
    30841 ttgcccgcag ggtaatgaga ttccggtggt cgccgttgcc agccatgata ccgccagcgc
    30901 ggttatcgcc tcgccgttaa acggttcacg cgccgcttat ctctcttctg gcacctggtc
    30961 attgatgggc ttcgaaagcc agacgccatt taccaatgac acggcgctgg cagccaacat
    31021 caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg
    31081 gctgcttcag cgagtgcttc aggaacggca aatcaacgat ctcccggcgc ttatcgccgc
    31141 gacacaggca cttccggcct gccgcttcat catcaatccc aatgacgatc gctttattaa
    31201 tcctgaggcg atgtgcagcg aaattcaggc tgcgtgtcgg gaaacggcgc aaccgatccc
    31261 ggaaagtgat gctgaactgg cgcgctgtat tttcgacagt ctggcgctgc tgtatgccga
    31321 tgtgttgcat gagctggcgc agctgcgcgg tgaagatttc tcgcaactgc atattgtcgg
    31381 cggcggctgc cagaacacgc tgctcaacca gctatgcgcc gatgcctgcg gtattcgggt
    31441 gatcgccggg cctgttgaag cctcaacgct cggcaatatc ggcatccagt taatgacgct
    31501 ggatgaactc aacaatgtgg ataatttccg tcaggtcgtc agcaccaccg cgaatctgac
    31561 cacctttacc cctaatcctg acagtgaaat tgcccactat gtggcgcaga ttcactctac
    31621 acgacagaca aaggagcttt gcgcatgacc actcaactgg aacaggcctg ggagctggcg
    31681 aaacagcgtt tcgcggcggt ggggattgat gtcgaggagg cgctgcgcca acttgatcgt
    31741 ttacccgttt caatgcactg ctggcagggc gatgatgttt ccggttttga aaacccggaa
    31801 ggttcgctga ccggtgggat tcaagctact ggtaattatc cgggcaaagc gcgtaatgcc
    31861 agtgagctac gtaccgatct ggaacaggct atgcggctga ttccggggcc gaaacggctt
    31921 aatttacatg ccatctatct ggaatcagac acgccagtct cgcgcgacca gatcaaacct
    31981 gagcacttca aaaactgggt tgaatgggcg aaagccaatc agctcggtct ggattttaac
    32041 ccctcctgtt tttcgcatcc gctaagcgcc gatggcttta cgctttccca tgccgacgac
    32101 agcattcgcc agttctggat tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt
    32161 ggcgagcaac tcggcacacc atcggtgatg aacatctgga tcccggatgg tatgaaagat
    32221 atcaccgttg accgtctcgc cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc
    32281 agcgagaagc taaaccctgc gcaccatatc gacgccgttg agagcaaatt gtttggcatt
    32341 ggcgcggaga gctacacggt tggctccaat gagttttacc tggggtatgc caccagccgc
    32401 cagacggcgc tgtgcctgga cgccgggcac ttccacccga ctgaagtgat ttccgacaag
    32461 atttccgccg ccatgctgta tgtgccgcag ttgctgctgc acgtcagtcg tccggttcgc
    32521 tgggacagcg atcacgtagt gctgctggat gatgaaaccc aggcaattgc cagtgagatt
    32581 gtgcgtcacg atctgtttga ccgggtgcat atcggccttg acttcttcga tgcctctatc
    32641 aaccgcattg ccgcgtgggt cattggtaca cgcaatatga aaaaagccct gctgcgtgcg
    32701 ttgctggaac ctaccgccga gctgcgcaag ttggaagcgg cgggcgatta cactgcgcgt
    32761 caggcactgc tggaagagca gaaatcgttg ccgtggcagg cggtatggga aatgtattgt
    32821 cagcgtcacg ataccccagc gggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa
    32881 gaaattttga gtcagcgtgg gtaaacactg ccagatgcgg cacgagcgcc ttatccggcc
    32941 tacagatcgt gcatctgaca acaccccatt tacaggaaca ccgaacatgc aaaacattac
    33001 tcagtcctgg tttgtccagg gaatgatcaa agccaccacc gacgcctggc tgaaaggctg
    33061 ggacgagcgc aacggcggca acctgacgct acgcctggat gacgctgaca tcgcaccgta
    33121 tcacgacaat ttccaccaac aaccgcgtta tatcccgctc agccaaccca tgcctttact
    33181 ggcaaataca ccgtttatcg tcaccggctc gggcaaattc ttccgtaacg tccagcttga
    33241 tcctgcggct aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct accatattct
    33301 ctgggggtta accaacgagg ccgttccaac ttctgaactt ccggcacact ttctctccca
    33361 ttgcgaacgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccatgccac
    33421 caacctgatc gccctcacct atgtactgga aaacgacacg gcggtcttca ctcgccaact
    33481 gtgggaaggc agcaccgagt gcctggtggt attcccggat ggcgttggca ttctgccgtg
    33541 gatggtgccc ggcacggacg aaatcggtca ggcgaccgca caagaaatgc aaaaacattc
    33601 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacactgg atgaagcctt
    33661 tggtttgata gataccgcag agaaatcagc ggaggtgtta gtcaaaatct actcgatggg
    33721 cggtatgaag cagaccatta cccgtgagga gctgattgca ctcggtgagc gttttggcgt
    33781 tacgccactg gccagtgcgc tggcacttta atatcccaac agatatccgg cggtgtggga
    33841 aacgtcgccg gacagatgga tttgaactta aggagagtca ttatgagttt tatgttggca
    33901 ttaccgaaaa ttagcctgca tggtgctggg gcaattggcg atatggtaaa tctggttgcg
    33961 cataaacagt ggggaaaagc actgatcgtt accgatgggc aactggttaa gttgggatta
    34021 ctggacagcc tgtttatcgc actggatcaa tacgaaatgt cttatcactt atttgatgag
    34081 gtatttccta accccactga agaactggtg caaaaaggct acgctgcatt tcaggatgcc
    34141 gcatgtgact acattattgc tttcggtggc ggtagcccaa ttgatactgc aaaagccgta
    34201 aaaatcctta ccgctaatcc tgcgccttct acggcgtact ctggtgttgg taaagtgaca
    34261 aatcccggcg taccgctggt ggcaatcaat acgactgccg gaacagctgc tgagatgacc
    34321 agcaatgcgg tgatcatcga caccgcgcgg caggtaaaag aggtcattat cgacccgaat
    34381 attattcccg atattgcggt ggatgatgcc agtgtaatgc tggagatccc ggcctcggtt
    34441 accgccgcca cgggtatgga tgcactgact cacgcagttg aagcttatgt ttctgttggt
    34501 gcacatccat taaccgacgc taacgcactg gaagctatcc gtttaattac gctgtggctg
    34561 ccaaaagcgg tagatgatgg tcataatctg gaagctcgtg aacaaatggc attcgcccag
    34621 tacctggcgg gtatggcatt taacagtgct ggtctgggcc ttgttcatgc acttgcgcat
    34681 cagcccggcg ccacccacaa cctgccgcat ggtgtgtgca atgctatctt gttgccagtt
    34741 attgaaaact ttaaccgccc gaatgccatc gcacgctttg cccgcattgc tcaggcaatg
    34801 ggtgtcgata cgcgtgatat gagtgaagaa gcggcaagta ctgaagcaat taacgctatc
    34861 cgcgcattaa gccatcgcgt gggtattccg gcaggattca gccagcttgg catcaccaaa
    34921 gaagatattg aaggctggtt agataaagcc ctggctgacc cgtgtgtgcc atgtaacccg
    34981 cgtaccgcca gccgcgacga ggtgcgcgag ctgtatctgg aggcattatg atccgtaagg
    35041 catttgtgat gcaggttaac cccgacgctc acgaagagta tcaacgtcgg cataacccca
    35101 tctggccgga actggaagca gtgttgaaat ctcacggtgc gcataactac gccatttacc
    35161 tcgacaaagc gcgtaatctg ctgtttgcca cggtagagat tgaatctgaa gagcgctgga
    35221 atgcggttgc cagtactgat gtttgccaac gttggtggaa atatatgacc gacgtcatgc
    35281 ccgcgaaccc ggataacagc ccggtgagta gcgaactgca agaagtgttt tacctgccgt
    35341 aattgcctaa tgtgccggag acgcctccgg tacaatattt ttaatttcga tttgaaaaat
    35401 aaacatatta caccttcagt taatatatct cggtaactta tatttccccg atgcattgtg
    35461 agtaaatcac aaaaataatg aataactcat taatgattca tgtggtttat ttaaataacc
    35521 attatgtgca ttactccgca aatctggcct ttcactctgt ctcaaattgt cattgtttac
    35581 cgacatatca cctctcatca cttaatcgct ccaccattac aattgattaa aatatatttt
    35641 atggagtccg attaatggca gctcttactg caagctgtat tgacctgaat attcagggca
    35701 atggcgctta ttccattctg aagcagttgg cgacaatggc gttacaaaac ggttttatca
    35761 ccgactcaca ccagttcctg caaaccctgc tgctgcgcga aaaaatgcac tctactggat
    35821 ttggttccgg catcgccgtg ccgcacggta aaagcgcctg cgttaaacaa ccgttcgtgt
    35881 tattcgcccg caaagcgcag gccattgact ggaaagccag cgacggcgaa gacgtcaatt
    35941 gctggatctg cctcggcgtg ccgcaaagtg gcgaagagga tcaggtcaaa atcatcggca
    36001 cactgtgtcg caaaattatc catcaggatt ttatcaacca actgcgacag ggcgacgccg
    36061 accaggtact tgctttgtta aatcaaaccc tcagctcata aggaagtggc gatggagtca
    36121 tccttacgta ttgtcgcgat caccaactgc cccgccggga tcgctcacac ctacatggtg
    36181 gcggaagccc tggaacagaa agcccgttct ctcggccata ccataaaagt ggaaactcaa
    36241 gggtccagcg gcgttgaaaa ccgcttatcc agcgaagaga ttgccgctgc cgattacgtc
    36301 attctagcta ccgggcgtgg cctgagcggt gatgaccggg cgcggtttgc cgggaagaaa
    36361 gtttatgaga ttgccatctc ccaggcgctg aaaaatatcg accagatttt cagcgaatta
    36421 ccaacaaact cgcagctttt tgccgcagat agcggcgtga agctcggcaa acaggaagtg
    36481 caaagcggca gcgtgatgag ccacctgatg gcgggcgtct ctgcggcgct gccatttgtc
    36541 atcggcggcg gtattctggt ggcgcttgcc aacatgctgg tgcagttcgg tttgccatat
    36601 accgatatgt cgaaaggtgc gccgtcgttt acctgggtag ttgaatccat cggttacctc
    36661 ggcttcacct ttatgatccc catcatgggc gcttatattg cctcgtcgat tgccgataaa
    36721 cccgctttcg ctccggcatt tctggtttgc tatctggcga acgataaagc cctgctcggc
    36781 acccagtcag gcgcgggctt cctcggcgcg gtggtgctgg ggctggcgat tggctatttc
    36841 gtcttctggt tccgtaaagt ccgtcttggc aaagcattac aaccgctgct cggctccatg
    36901 cttatcccat tcgttactct gctggttttt ggcgtgctca cttactacgt tatcgggccg
    36961 gtgatgtccg acctcatggg cgggctgctc cacttcctga ataccattcc tccatcaatg
    37021 aagtttgcgg cagcgtttct ggtcggtgcg atgctggcgt tcgatatggg cggccccatc
    37081 aataaaaccg cctggttctt ctgcttctca ctgctggaaa aacacattta tgactggtac
    37141 gccatcgtcg gcgttgtcgc gctgatgccg cccgtcgcgg cgggtcttgc gacgttcatt
    37201 gcgcccaaac tgtttacccg acaggaaaaa gaggctgcca gtagtgccat tgtggtcggt
    37261 gccacagtcg caaccgaacc ggctattcct tacgctctgg cagctccgtt gccgatgatt
    37321 accgccaata cgctggcggg cggtattacc ggcgtgctgg tgatcgcttt cggaatcaaa
    37381 cgtctggcac ctggtttggg tatctttgac ccgctgatcg gcctgatgtc accggtaggt
    37441 tcgttttatc tggtgctggc gattggcctc gcgctgaaca tttcattcat tatcgtcctg
    37501 aaaggattgt ggttacgccg caaagcgaaa gccgcgcaac aggagcttgt ccatgaacat
    37561 tgagttactg caacagttgt gcgaagccag cgccgttagc ggcgatgaac aggaagttcg
    37621 cgacattctg ataaacacgc tggaaccttg cgtgaatgaa atcacctttg atgggctggg
    37681 cagctttgtc gcccgtaagg gaaataaagg gccgaaagtt gccgttgtcg ggcatatgga
    37741 tgaagtcggc tttatggtca cccacatcga cgagagcggt tttctgcgtt tcaccaccat
    37801 tggtggctgg tggaatcagt cgatgctcaa ccaccgggta accatacgca cacacaaggg
    37861 agtgaaaatc cctggtgtga ttggttccgt cgcgcctcat gcgttaacgg aaaagcaaaa
    37921 gcaacagccg cagtcatttg atgagatgtt cattgatatt ggcgcaaaca gtcgcgatga
    37981 ggtggaaaaa cgcggtgtgg cgatgggcga ttttattagc ccggaagcca attttgcctg
    38041 ctggggcgaa gataaagtgg tcggcaaggc gttggataac cgcatcggct gcgcaatgat
    38101 ggctgagcta ctacagacgg tgaataatcc cgaaattacg ctgtatggcg ttggcagtgt
    38161 ggaagaagaa gttgggctac gcggggcgca aacctcggct gaacacatta aaccggatgt
    38221 ggtgatcgtg ctggacaccg ctgtcgcggg cgatgttcca ggcattgata acattaaata
    38281 tccgctgaaa ctgggcaacg ggccggggtt gatgctgttt gacaagcgct acttccccaa
    38341 ccagaaactg gtagcagcgt taaaaagctg tgccacgcag aatgatttac cgctgcaatt
    38401 ttccaccatg aaaaccggtg cgacggatgg cgggcgctac aacgtgatgg gcggtgggcg
    38461 tccggttgtc gcgctgtgtt tgccaacgcg ttatctgcac gccaacagcg gcatgatttc
    38521 aaaagccgat tacgatgctc tgctcacgct gatacggggt tttctgacca ccttaaccgc
    38581 ggaaaaagtc agcgcgttta gccagttccg tcaggtggat taatgctaaa cgaacgccag
    38641 ttaaagattg tcgatctgct ggagcaacag ccgcgcacgc ctggcgagct ggcgcaacag
    38701 actggcgttt ctggcaggac catactgcgt gatattgact atctcaactt tacccttaac
    38761 ggcaaagccc gcatttctgc cagtggtagt gcgggctatc agctggaaat cttcgagcgc
    38821 cgcagctttt ttcagttgct gcaaaagcac gataacgacg atcggctgct ggcactgtta
    38881 ttactgaata cctttactcc ccgtgcgcaa ctcgcctcgg cgcttaattt gccggaaacg
    38941 tgggtcgcag agcgtctgcc ccggttaaaa cagcgttatg aacgcacttg ctgcctggcc
    39001 agtcgccccg gtttgggcca tttcattgat gagacagaag aaaaacgcgt tatattgctg
    39061 gcgaacttgc tgcgcaaaga tccgttttta attccgctgg cgggcgtaac acgagacaac
    39121 cttcagcatt tatccacggc ctgcgacaac caacaccgct ggccgctcat gcagggtgat
    39181 tatctctcca gcctgattct ggcgatttac gccctgcgta atcaactgac cgatgagtgg
    39241 ccgcaatatc ccggtgacga gataaaacaa atcgttgaac atagcggtct gtttcttggt
    39301 gataacgctg taagaaccct gacaggtttg atagagaaac agcatcagca agcgcagata
    39361 atttctgccg ataatgtgca gcggttgctg caaagggtgc cgggcatcgc gtcattgaat
    39421 attattgata cgcagctggt tgagaacatt accgggcatt tattacgttg ccttgccgca
    39481 ccggtgtgga ttgctgagca ccgccaaagc agcatgaata acctgaaagc cgcctggcct
    39541 gcggcctttg atatgagtct gcactttatt acgctactgc gtgaacagct cgatattccc
    39601 cttttcgaca gcgatctgat cggtttgtat tttgcctgtg cgctggaacg gcatcaaaac
    39661 gaacgccagc cgatcatttt gctctcggac cagaacgcga ttgccactat taatcagctc
    39721 gccattgagc gcgatgtttt acattgtcgg gtgattattg cccgtagctt aagcgaactt
    39781 gttgccattc gcgaagagat tgagccgtta ttgatcatta acaacagcca ttatttactg
    39841 gatgacgcgg taaataatta catcaccgta aaaaatatca ttacggctgc cggtatcgaa
    39901 caaataaaac attttctggc gacggcattt attcgccaac agccggagcg ttttttctct
    39961 gctcccggaa gttttcatta ttcgaatgta cgcggtgaaa gctggcaaca tattacccgg
    40021 caaatttgtg cgcaattagt cgcacaacac catattaccg ccgatgaagc acaacgcatc
    40081 atcgcccgcg aaggcgaagg tgaaaacctg attgttaatc gcctcgccat cccacattgc
    40141 tggagcgaac aggagcgacg ttttcgtgga ttttttatta ccctcgccca accagttgag
    40201 gtgaataacg aagtcattaa ccatgtcttg atcgcctgcg ccgccgccga tgcgcgtcat
    40261 gagctaaaaa tatttagcta tctggcaagc atattgtgtc agcatccggc ggagattatt
    40321 gccgggttaa caggatatga ggcatttatg gagttacttc acaaggggtg aactgtgtcg
    40381 gatgcgccgc atccgacact gcatttatca tcaaaaacgt ttaacgcagg ctattatagt
    40441 catcaaccat ttcgttgtac tcgcgtaatg ctctcgggaa agagtcttcg accatccagc
    40501 ttgagtttgc gtcctgaagt tgctctttat cccagtcaga gtacgggact ttatctctga
    40561 tgcggcgaac gtatttttta gcctcgagtt gatactggcc tgccgaatta ataaacgaga
    40621 aattcatgcc gcctttatcc gcttctttgg cctgcgccac tagcgtttcc agttcagaca
    40681 ctttcttcat tgccgcatca acatcaaact tctcttgcga tatcaggtta ttaatttgtt
    40741 tcgctgaaat catgactgcc agagagtagt agtggaatgt tttcccttct ctttcttcaa
    40801 tatttttcag ttccgcaagc tggcgcttat cgttaatttc ctgaatcgcc gcatgataag
    40861 attctgctac aggttcaaag gcttccagat tttttaagaa tgtctggtgc agcgttttcc
    40921 ctttcgcaaa cgcatcgtct ttataatttt cctgggtgta atatttatcc atttcgttaa
    40981 tggtattacc caacgccacg gcagcatcga tataactcac cgccacgcca tcaattggtt
    41041 gcagcgcagg cgttaacgcc acagcacttt ttacgccttt ttcacactca gcgaggttgg
    41101 attcactaat gccgtagatg ccataaacag tacgctcttc accggtaggc ccctgtttaa
    41161 aatctttcag ccagtcagca taacgcgcca gactgctctg tacagggatc tgtaacttgt
    41221 tatagcactt gatatagaca tccatttttt cgtcgaaaac cgcttgttca tcgaccaccg
    41281 gttgcgcagg tgtttcaggt ttagcttctg cttttgcaac gggagcttca ggagctggtg
    41341 cggcaggttg actattggca ggcgggggcg tttctgtttc ggctttttta tcatcacaac
    41401 ccgtcagacc gagggccatg atggcgacta taatagcgga agataataaa ttccttttca
    41461 tt
//
LOCUS       DAZFJF010000028        41326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFJF010000028 DAZFJF010000000
VERSION     DAZFJF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.028"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..459)
                     /locus_tag="ACHKAU_001689"
     CDS             complement(<1..459)
                     /locus_tag="ACHKAU_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5530775.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGF"
     gene            complement(719..1972)
                     /gene="amiC"
                     /locus_tag="ACHKAU_001690"
     CDS             complement(719..1972)
                     /gene="amiC"
                     /locus_tag="ACHKAU_001690"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO5530776.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2204..3535
                     /gene="argA"
                     /locus_tag="ACHKAU_001691"
     CDS             2204..3535
                     /gene="argA"
                     /locus_tag="ACHKAU_001691"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO5530777.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3597..5423)
                     /gene="recD"
                     /locus_tag="ACHKAU_001692"
     CDS             complement(3597..5423)
                     /gene="recD"
                     /locus_tag="ACHKAU_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO5530778.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5423..8965)
                     /gene="recB"
                     /locus_tag="ACHKAU_001693"
     CDS             complement(5423..8965)
                     /gene="recB"
                     /locus_tag="ACHKAU_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO5530779.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8958..11846)
                     /gene="ptrA"
                     /locus_tag="ACHKAU_001694"
     CDS             complement(8958..11846)
                     /gene="ptrA"
                     /locus_tag="ACHKAU_001694"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO5530780.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12022..15390)
                     /gene="recC"
                     /locus_tag="ACHKAU_001695"
     CDS             complement(12022..15390)
                     /gene="recC"
                     /locus_tag="ACHKAU_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO5530781.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWTHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15403..15726)
                     /gene="ppdC"
                     /locus_tag="ACHKAU_001696"
     CDS             complement(15403..15726)
                     /gene="ppdC"
                     /locus_tag="ACHKAU_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO5530782.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15711..16118)
                     /gene="ygdB"
                     /locus_tag="ACHKAU_001697"
     CDS             complement(15711..16118)
                     /gene="ygdB"
                     /locus_tag="ACHKAU_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO5530783.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16115..16678)
                     /gene="ppdB"
                     /locus_tag="ACHKAU_001698"
     CDS             complement(16115..16678)
                     /gene="ppdB"
                     /locus_tag="ACHKAU_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5530784.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16669..17139)
                     /gene="ppdA"
                     /locus_tag="ACHKAU_001699"
     CDS             complement(16669..17139)
                     /gene="ppdA"
                     /locus_tag="ACHKAU_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5530785.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17323..18117)
                     /gene="thyA"
                     /locus_tag="ACHKAU_001700"
     CDS             complement(17323..18117)
                     /gene="thyA"
                     /locus_tag="ACHKAU_001700"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO5530786.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18124..18999)
                     /gene="lgt"
                     /locus_tag="ACHKAU_001701"
     CDS             complement(18124..18999)
                     /gene="lgt"
                     /locus_tag="ACHKAU_001701"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO5530787.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19150..21396)
                     /gene="ptsP"
                     /locus_tag="ACHKAU_001702"
     CDS             complement(19150..21396)
                     /gene="ptsP"
                     /locus_tag="ACHKAU_001702"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5530788.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21409..21939)
                     /gene="rppH"
                     /locus_tag="ACHKAU_001703"
     CDS             complement(21409..21939)
                     /gene="rppH"
                     /locus_tag="ACHKAU_001703"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO5530789.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22292..22438)
                     /gene="ygdT"
                     /locus_tag="ACHKAU_001704"
     CDS             complement(22292..22438)
                     /gene="ygdT"
                     /locus_tag="ACHKAU_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO5530790.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22624..23313
                     /gene="mutH"
                     /locus_tag="ACHKAU_001705"
     CDS             22624..23313
                     /gene="mutH"
                     /locus_tag="ACHKAU_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO5530791.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23382..24095
                     /gene="ygdQ"
                     /locus_tag="ACHKAU_001706"
     CDS             23382..24095
                     /gene="ygdQ"
                     /locus_tag="ACHKAU_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5530792.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24233..24451
                     /gene="ygdR"
                     /locus_tag="ACHKAU_001707"
     CDS             24233..24451
                     /gene="ygdR"
                     /locus_tag="ACHKAU_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO5530793.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24559..25599
                     /gene="tas"
                     /locus_tag="ACHKAU_001708"
     CDS             24559..25599
                     /gene="tas"
                     /locus_tag="ACHKAU_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO5530794.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25631..26824)
                     /gene="lplT"
                     /locus_tag="ACHKAU_001709"
     CDS             complement(25631..26824)
                     /gene="lplT"
                     /locus_tag="ACHKAU_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO5530795.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26817..28976)
                     /gene="aas"
                     /locus_tag="ACHKAU_001710"
     CDS             complement(26817..28976)
                     /gene="aas"
                     /locus_tag="ACHKAU_001710"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO5530796.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29562..30593
                     /gene="galR"
                     /locus_tag="ACHKAU_001711"
     CDS             29562..30593
                     /gene="galR"
                     /locus_tag="ACHKAU_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO5530797.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30600..31862)
                     /gene="lysA"
                     /locus_tag="ACHKAU_001712"
     CDS             complement(30600..31862)
                     /gene="lysA"
                     /locus_tag="ACHKAU_001712"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO5530798.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31984..32919
                     /gene="lysR"
                     /locus_tag="ACHKAU_001713"
     CDS             31984..32919
                     /gene="lysR"
                     /locus_tag="ACHKAU_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO5530799.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32906..33598)
                     /gene="ygeA"
                     /locus_tag="ACHKAU_001714"
     CDS             complement(32906..33598)
                     /gene="ygeA"
                     /locus_tag="ACHKAU_001714"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO5530800.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33727..35145)
                     /gene="araE"
                     /locus_tag="ACHKAU_001715"
     CDS             complement(33727..35145)
                     /gene="araE"
                     /locus_tag="ACHKAU_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO5530801.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(35460..36221)
                     /gene="kduD"
                     /locus_tag="ACHKAU_001716"
     CDS             complement(35460..36221)
                     /gene="kduD"
                     /locus_tag="ACHKAU_001716"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO5530802.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(36251..37087)
                     /gene="kduI"
                     /locus_tag="ACHKAU_001717"
     CDS             complement(36251..37087)
                     /gene="kduI"
                     /locus_tag="ACHKAU_001717"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO5530803.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(37374..38555)
                     /gene="yqeF"
                     /locus_tag="ACHKAU_001718"
     CDS             complement(37374..38555)
                     /gene="yqeF"
                     /locus_tag="ACHKAU_001718"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO5530804.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38810..40039
                     /gene="yqeG"
                     /locus_tag="ACHKAU_001719"
     CDS             38810..40039
                     /gene="yqeG"
                     /locus_tag="ACHKAU_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5530805.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            40499..41131
                     /gene="yqeH"
                     /locus_tag="ACHKAU_001720"
     CDS             40499..41131
                     /gene="yqeH"
                     /locus_tag="ACHKAU_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5530806.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQY"
BASE COUNT        10366 a        11089 c         9938 g         9933 t
ORIGIN      
        1 aaaaccaatc tcatcatccg gtagttgaac gctaaattgc tcattaatga ttgttaatgc
       61 atcacgactt attgcatact ctgctttgta caacgctttg atttcctcaa ggaaaacatt
      121 attaatttgt atgcctgaga cgactcggga tattgcattc gaaatgtgat cagcaagaat
      181 cagtagtagc gatggattga gttttttttc gagattactc tccgcataat gcactatctt
      241 ttcggcaaca aaaatatact caatccccac atgctcaatc aatttgtaga gtttattttt
      301 ttgctcattt ctgacataaa aaatccggtt tgcaggatgc tcagggaccg acataccata
      361 ttttttattg taaccaacgc caggcccaga aatgataacc tcctgtccgt tcaccgatgc
      421 ctgtacacaa ttattgttca tgactttttc gatgatcatt ccatggctcc caaaaaaaaa
      481 ggcaaggcat gtcagaagta ataattactt caaacattgc cttgcctgat ttaacagtta
      541 caagataatt aactaatgcg tataccttac acggaattgt gcttaataaa agagatgaca
      601 ggcttatttt ttaagccgat attcaaatat tcgacagcag tcacaaaacc atcgttcaac
      661 atttatgtaa ataatattta ttcaatgaaa cataaattaa ttgaataaat taaaaacatc
      721 atccccttct cgccagcgtc gccccatcgg caaaatacgc tttaatcccc gcaagaatag
      781 actccgcaac ttcctgctga aaggtcgccg ttttcagttt acgctcttcc tcaacgttac
      841 tgataaacgc cgtttcgacc agaatggagg gaatatctgg tgcctttagt acggcaaacc
      901 cggcctgttc aacctgattt ttatgcagct tgttgatttt accgagctta ttcagtaccg
      961 ctttaccaaa cttaaggctg tcagcaatgg tcagcgactg caccatatcg aacatggtgt
     1021 ggtcgacata gcggtcaccg cttttgctca cgccaccaat caagtccgag gcgttctggg
     1081 tttgtgccag atattttgcc gcagtactgg ttgcaccttt ggttgagagc gcaaacacag
     1141 aggaaccgct cggctgacga ctggtaaagg cgtcggcatg gatagagaca aacaggtcag
     1201 cacgctgttt ctgggctttt gctacgcgca cttgcaacgg aatgaagatg tcttcattgc
     1261 gcgtcatgta caccttcata ttgccctctt tctcgatcag agagcgcaaa cggcgagcta
     1321 tttgcaatac cacatctttt tctcgcgttt tgtatttccc caccgcaccg gagtcttcgc
     1381 caccgtggcc agggtcaagc ataatgacaa tcggacgatc gcgccctgct ttacccggtt
     1441 gtggaccact ttgtgctggc ggcacctgct tttcgaggtc gcctttgttg taatcctcca
     1501 gcagcgccag cagcgggtcc tgcatatcct gtgcattggc cggatagagg tccatcacca
     1561 gacgctcttt aaacccggcg accggcgcaa gggcaaacag ctgcggtttt acgttttgct
     1621 ttaattcaaa aaccatccgt acggtctgcg ggtcaaattg cccgacgcgc gccgacttga
     1681 tgaacgggtc gtctgcacga atttgtgccg ccatcccctt gagtaccgag ttcaggttta
     1741 catcttcgat atccaccacc acgcgttccg ggttactcaa cgcgaactgc ttatatttca
     1801 gctgacgatt tgattctacc gtcacacggg tgtagctgga cgcaggccag acgcgcaccg
     1861 ccacgacctg gctgaccgca gccagactga cctgacttac gctcaatagc cacatggcac
     1921 ccgcgccttg cagtaaacga cgacggctga ttgcagtgtt ggatcctgac atgcctctcc
     1981 cgagcaaaag aaatctaagc tgtgtaacaa gtaaacgact aatttgaccg gaaactttag
     2041 cgaaagacgc ataatctgtc atctaataaa cggtaaacat tctttttata ttcacggcat
     2101 tactgataaa aaagtcgctc tcgcataaaa tttacacttg caccctgcga aaaaacagaa
     2161 taaaaataca ctaatttcga ataatcatgc aaaggggtgt gccgtggtaa aggaacgtaa
     2221 aaccgagctg gtcgagggat tccgccattc ggttccctat atcaataccc accggggaaa
     2281 aacgtttgtc atcatgctcg gcggtgaagc cattgagcat gagaatttct ccagtatcgt
     2341 taatgatatc gggttgttgc acagcctcgg catccgtctg gtggtggtct atggcgcacg
     2401 tccgcagatc gacgcaaatc tggccgcgca tcaccacgaa ccgctgtatc acaagaatat
     2461 acgtgtgacc gacgccaaaa cactggaact ggtgaagcag gctgcgggaa cattgcaact
     2521 ggacatcact gctcgcctgt cgatgagtct caataacacg ccgctacagg gcgcgcatat
     2581 caacgtcgtc agtggcaatt ttattattgc ccagccgctg ggcgtcgatg acggcgtgga
     2641 ttactgccat agcgggcgta tccggcggat tgatgaagac gcgatccatc gtcaactgga
     2701 cagcggtgca atagtgctaa tggggccggt cgctgtttca gtcactggcg agagctttaa
     2761 tctgacctcg gaagagattg ccactcaact ggccatcaaa ctgaaagctg aaaagatgat
     2821 tggtttttgc tcttcccaag gcgtcactaa tgacgacggt gatattgtct ccgaactttt
     2881 ccctaacgaa gcgcaagcac gggtagaagc ccaggaagag aaaggcgatt acaactccgg
     2941 tacggtgcgc tttttgcgtg gcgcagtgaa agcctgccgc agcggcgtgc gtcgctgtca
     3001 tttaatcagt tatcaggaag atggcgcgct gttgcaagag ttgttctcac gcgacggtat
     3061 cggtacgcag attgtgatgg aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat
     3121 tggcggtatt ctggagttga ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc
     3181 tcgcgagcag ctggagatgg aaatcgacaa attcaccatt attcagcgcg ataacacgac
     3241 tattgcctgc gccgcgctct atccgttccc ggaagagaag attggggaaa tggcctgtgt
     3301 ggcagttcac ccggattacc gcagttcatc acggggcgag gttctgctgg aacgcattgc
     3361 cgctcaggcg aagcagagcg gcttaagcaa attatttgtg ctgaccacgc gcagtattca
     3421 ctggttccag gaacgtggat ttaccccagt ggatattgat ttactgcccg agagcaaaaa
     3481 gcagttgtac aactaccagc gtaaatccaa agtgttgatg gcggatttgg ggtaagcgat
     3541 gaaaatcgcc ggatgcgaca tgcgtaacac tcgtgcgtcg catcaggcaa ttacgtttat
     3601 tcccgcgaac taaacaatgc cgccagacca ctgcgccgct cagtgcgcgt ggcgattgcc
     3661 gcacttaata tgcgctcatc ggcatacagc gacagacggc gacgcgcgcg ggtcactgcg
     3721 gtgtaaacca gctctcgcgt tactaccggc gtgcgttggc tcggtaaaat caacgccgca
     3781 tggtcgaact ccgatccctg cgatttatgt accgtcatcg cccacgtcgt ttcgtgctct
     3841 ggcaggcggc tcggttgcac agacttaata ttgccgtccg gcatcgcaaa ccaaacgcgc
     3901 gtcccctgcc cgcgatccag cgcaataccg atatcgccat taaacaaccc aagcgcgctg
     3961 tcattacggg caatcatcac cggtcgacct tcgtaccaac gagagtgcgg atgacgatga
     4021 attttgcgct tctgttgcat aaactgctca attcgctcat tcagtccagc cacgccaaac
     4081 ggcccttccc gcagggcgca caaaagttgg tactcattga acgcctgaat gattaaatcc
     4141 ggctcggcac gcgcttgcag cagatccaga taacgtccat aacccgcaag agcttcctca
     4201 agcatcgcaa tataatcttc tccgctctgt aaaagtcgtt tttcgatatc agtaaaatcc
     4261 tgctgaaaaa cggttttcac tgccgtttta tcaccacggt tgatcgccgc agctaactga
     4321 ccaatgccag aatcgctgcc gaaacgatag cttttttgca gcaggcagag actgtcgcgc
     4381 aaagatgccg cttctgtgcc ggttcctgcc ggaacgtgag tccccgtcag gcggcttagc
     4441 tgcctggcac gctcggcggt aaagcccgcg ttggcataag cgcagatatc gcccagcaca
     4501 gccccagcct caaccgaggc cagttgatca cgatcgccga gaaagatcac tcgcgcatga
     4561 tctggcaagg cgtcgatcag tctcgacatc ataggcagat cgatcattga cgcttcatct
     4621 accaccagca catcaagatg cagcgggtta ccggcatgat gacgtaaacg ctggctaccc
     4681 ggctgcgcgc ccagcaatcg gtgcaaagtg ctggcatctt ccggaatgcg tttcttttgt
     4741 tcatcggtca gcggtaactg tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca
     4801 gctttacccg ttggtgcagc cagacggata cggcagcgtt cgccgtcggc catttgaatt
     4861 aacgctgcca gcaacttcgc cacggtggtc gttttaccgg tgcctgggcc gccggaaacc
     4921 accgagatcc gccgcgtcag cgccactgcc gctgcaactt tttgccagtt aatttcatcg
     4981 cttactggaa aaagtttgtc cagggtttgc gccagtagag cttcatcaac ctcaatggca
     5041 tgattcacct cattgaagaa gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa
     5101 taaagacgat cgccacagag gaccatcggc gtgggttcat ctcccctgct gaccgcttga
     5161 gaagccagca agcattcttc ccaattttgt agctcaccga tttcactgac acaggtcgcc
     5221 aacagcggat gcgacgcctc gttattttcc agtcgtgaaa gcggcaaaca gacgtgtccc
     5281 tctccggcat catgacttaa cagtgccgcc gcgagggtga cggcaggatg ttcatctccc
     5341 gccacggtca gggcaaattg cacatccagc gggcgtagct gtttgtgctc cacagcttcc
     5401 agtaattgct tttgcaattt cattacgcct cctccagggt cataccggca aacatctcat
     5461 ccatcagggc aatcaacccg gcgttgggtc gggttgtgta aatcccttgt tgcggatgtt
     5521 ctttatcaac gccacgcagg aacagataga taacgccgcc aaagtgacga tcatagtcgt
     5581 aatcagcaat gcgatggcgc agataacgat gcagcgccag ggtataaagc tgatattgca
     5641 gatcatagcg gtgtgcctgc atcgctgctg ccatagcctg ttgggtgtaa gccgaactgt
     5701 cttcacccag ccagttggat ttatagtcga gcaggtaata acgcccttcg tggcggaata
     5761 ccaggtcgat aaagcctttt aacatgccgc gtacctgcat gaactccagc ggcgggcagc
     5821 cagcggatag cgggtcaaac tggcgaatta gtgcatcaag ctggctggcg atgagcggtt
     5881 cgctaatcgg caggtaaaac tccatctcca cctgtttatc gcggtcggaa agctgactca
     5941 ggctaacacc cgtttcattg agaggtgcct ggaggacagc cgtgatccac tcggtcaaca
     6001 ccggttccca ctgcgattca aagccgccga gttccagttt ttcctgtacc cagttcgggt
     6061 caaccggctg ggtaaaatcg aggtcttcaa acaaactgtg caagaatgtc cccggtgacg
     6121 caccgcgcgg gaactgatgc ggtgttaacg tcggttcttc aacgacgctg accacgcctg
     6181 cggcatcaac atccagccgt ggcatcagat cctgagcgat accgtgacca cgctgctgca
     6241 agccggagta gctggtgaca cgccagttat cgccgggcaa tcgttgtagc gtcctcgcat
     6301 tcagttctgc agttaaagca tcattaacct gccagggctg gttatcacca gtttgtgccg
     6361 tttgccaggc aatatcatca tcgcataacg cttcaataca ggtgcgaagc cctgccgcat
     6421 cttgcggttc ccctttttgc agcaaacgcc cgagcgcact ttggtggacg tcggtgtcac
     6481 cttttttatc gccacgacgg cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg
     6541 aacgcgtcag cgccacgtaa agcaaacgca gatcttccgc cagacgttcg acctccgcaa
     6601 gctcgacgct ttctggcgca gcgttaagat ccagaacagc ctcaaacgag tggcgatcgt
     6661 gataaaacgc ctgatcctgg acgcggaaat tggtaataaa tggcaaccag accagcggat
     6721 attccagccc tttcgattta tggatcgtga caatctgcac cagatgttta tcactttcga
     6781 ggcgcatttg ttggctggag gcattactgt ctggctcgag gatatgttgc gataaccagc
     6841 gtaccagcgc atgttcactt tccagctgcg ttccggcttc ttgtagcagt tcgctgatat
     6901 gcaagatatc ggtaagacgc cgctcaccgc ctgccgttgc cagcaagttt tctgcaatgt
     6961 tacgcgccga catcagcgcc cgcagcatcg gcataacgcc acgtttgcgc cagatttgcc
     7021 gataaccatc gaactcttca actaccgcat cccacgcatg ttcgtcatta ttcagcgttt
     7081 cgatatccag cgcgttcagc cccatcattg acgttgccag cgcactgcgc agggtgttct
     7141 cacgttcggg cgtcatcacc gcctgcaaca accaaagcat ttcctgcgct tccagagttt
     7201 caaaaacact gtcgcggttc gaaaggtaaa cggaagggat ttccagcaac gttaaggcat
     7261 cgcgcacctg ggcggcctcc tggcggctgc gcaccagcac actgatgtcc gaagcacgca
     7321 ccggacgcgc gtcgtcgccg ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc
     7381 agtcgcggat ttgcgcagca catacctgcg ccatggtact ttgataatcg ccaacgccgc
     7441 agctttcgcc ttccatcagc cacattttca tcgcaggctg cgtttcacct ttaaacacaa
     7501 aacgtaacgc ctgatttttc ccggctgatt tcactggaat aaacggtatt tcgcgaaaca
     7561 tgaacgcgtc atcagtctgg ctgaaaagct tgttcacact gttcaccatt ccaggtgcgg
     7621 agcgccagtt ggtgtctaaa gtgtagtggg cgtgaacttc actacgcgcc ttcatataag
     7681 tgaagatatc cgcaccccgg aatgcatata tggcctgctt cgggtcgcca atcagcaaca
     7741 atgcggtttc cggctgatgg tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg
     7801 tatcctgaaa ttcatcgatc attgccaccg ggaatcgcgt acggatcgcc gctgccaaca
     7861 cctcaccgct ttcgctacgc agcgcggaat cgagccgact taacatgtca tcaaaaccca
     7921 attcgccacg gcggcgtttt tcacgcgcta ctgtttcgcg gatctcagcc aatgcgcggg
     7981 tgatcaccag atcgcggatc gacaatggtt ctgcaagcag ttgatcgatc gcctcaaaca
     8041 gtggatgtcg cggggtttcc cccccggcct tcgtgcgatc ttctaagaaa cgctgggaga
     8101 atttttccag cgactccggc aactgataac tgtttgtctc ttcttctgcc caggcgctga
     8161 tcttttcgat ccatttagcc tgattgctac ggttaaactt gcgtcgatca ataccagaag
     8221 attcgatcag cgcatcaagt tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa
     8281 tacgcgccac aatttgcgcg tggcgggaag ccagcgtttc atcatcgggc ggcggtgctt
     8341 tgataaccgg tgcttcgcct tgcagataac gattaatatc gcgcagcaac gcctgcggcc
     8401 ctttccaggt ttcaaagacc accagggcaa tttcacgcgg cagcgggtag cagtggcgac
     8461 gccagaaatc ggcgcaggcc tggtagcgta gcagagactc atcttcaatc agctgctgct
     8521 caaacagcat accggattca aaggcattca ggttgagcat gcgctggcaa aaaccgtgaa
     8581 tagtaaagac tgccgcttca tccatctgcc gttcggctaa caacaaccac tgcgcggctt
     8641 gcgctttatc gtcgatctct tccagcaggc gttcgtacag tggattgtcg gtggtttcac
     8701 gcagacaggc gatgcgcaac tcgtggatat tgctacggat acgaccacgc aattctgccg
     8761 tggcagcctc ggtaaaggtg accaccagca gttcttcaac ggtcagcggg cggggaaagg
     8821 cggcggaacc gcctagtcca agtaacaggc gcaaatagag cgccgcaatc gtaaaggttt
     8881 tgcctgtgcc ggcagaggct tcaatcaggc gctcgccctg taagggcaag cgcaaaggat
     8941 ctagtgtctc ggcgacatca ctcattcttt tcactcatca ggggcattgt ttgctgcaac
     9001 gcgctgacgt tctcccacac tttccagcct tcaggatgca catattcggc tttcccgttc
     9061 tggctgccgg aaatctgcga cagaatagcc atgccttgcg gctcgaccac cgcctgatgg
     9121 aagaaatcag caagtttttg cggcgtcagc agttttatct gggccacgat tttatcacgc
     9181 gaatcgaagc gcatattgcc gcgatcgaaa tctttactta acttcgatgc ttcttcgccg
     9241 agcgtttgcg gtgcctgcag catctgggta attaccgcct gctggatttg cgcaaactct
     9301 tctggcttca tcgcccgcaa ttttgcctct gcggttggga aaaacgcctt gtaacgctcc
     9361 cacaagaagg aaggctgttt atcattgctt tgcaaaagga agcccatgcc ccactgacgc
     9421 cccacgctca ttggaaatgc aaacacggca tagcccaatt gttcttcggt acgcaactga
     9481 ttgtagaacc acggctgtac gatctgcccc aacagagagc tataggctga gctggtgtat
     9541 tcatcgtagc cagtcggtac aaataccgct gccagtgcgg agtcggtgct gttaccagct
     9601 ttttcaaaga tgacggattg ttttttatcg accacgacat ctttgttacg acaccactct
     9661 gaaccatcag cgcccaactg tttttgcaca tcgcgtgcca gcgttgttgc ctggtcctcg
     9721 gtcatgttgc cgataaccat aaactctggt cgagcccctg attttaaggc gtcgcgatag
     9781 gccagcacct ctttcaacgt aatggagggc aaaattttgc gccgttcatc tcgcgagaag
     9841 tacggcactt gcgagagcat ctgcgcgggc ataatcgcct gctcgaacgc tttgcccttt
     9901 tctgcggaat ccatcatctg gttataccag gacttcgcct gctcaagctg atcttccgtg
     9961 gcggtatagc taaagtagcc ttccagcaat gcctggaaca gctgcggcag atgctgggtg
    10021 taaccattag cattaaccat aaggccgttg ttagcgttgg tggaaaaact tatgccacca
    10081 accgacgcct ggttgcttaa ctgatcaagc gccagccctg cgagataatc attgagcgca
    10141 aacatcacct gattgcgggc gctgtccatg gctttcggat tacgcaaaat caggctgaca
    10201 tcggcttttg gctcgctggc aaaataacgg cttggcgcat acaccacgcg cagattcgac
    10261 tcatcaacaa tcagctctgg atggtcatat ttcttgtctg acttaatcag cgagaaatcg
    10321 tcaggaatat aagggttaag ctctggcaaa gagagcgcaa tgtcggcggc ttttttctgc
    10381 cagtcggcga aagtttgtgc gctgatttta tcgacctgat acggcgcatc gacaaagtaa
    10441 gccgttttgt tgtgcggctc tttcgggctg atataccaga tacgcgcatt ctgcggcgtc
    10501 atcatcgcca gacgttcctt tactgcttta gcatcgtacc gatcggcaat attgactgca
    10561 tccagcgtat gctcaacagg aacgcgaatc atggtatctg ccagccattc gacgtaatcc
    10621 atatcacggg tgattgacgg ataacggaag tcgatatcca gcacattcgc cagttcatcg
    10681 aagtattgtt tatcgatccc tttttcacgt aacaaattga ggtagctaaa aatagccgcc
    10741 acaacctgat cgcgattcgc cagaccttta tcggttaaag acgcagagat cgctaatacg
    10801 ccgctgttgc cgttgacgat aggatcggag ttggcgctaa tgccctcaac taatccctgc
    10861 ttttgcagcc agtcggaaag tgtacctggg ctgcgattac caatcagata ggtaatcaat
    10921 tcatcggttt tgctacggaa tttcgccgag ttgttatcaa tacgaaactc tacgcgcaac
    10981 actttacgcg gcagcgccgg gacgtaatga atgataatgc ccttttgcgc gtcggtgact
    11041 accggcacgg tgatttccgg ttttttgctc tctttgttcg gcacgcgacc aaaggtgtcc
    11101 gccgccattt ttgccaactc cggcagcggt ttattgctgt aaataaccgc cttcatcaga
    11161 ttggcggaat agtacttctc gtggaaatct ttcagcgcct gctgcaccgg attaccaggt
    11221 ttgtcgctta aagtttcgag gttaccacca gaaaactttg aaccggggtg tgccgggtta
    11281 atggtttctg cgctgacctg tgccatgcgc atcccgtcac gcgtacgcgc catggttaat
    11341 tcagcgttca ctgcattacg ctcacgttcg gcgtatttct tgtcgagcaa gggttctgca
    11401 atagcatcag ccaggcggtc taccgcacca ggcaaggcgt cgttctcaac ttccagatag
    11461 aaagccgtgc gatacggcgc agtgctggca ttgtgactac cgccgtgcat tttgagatat
    11521 tcggccagac tgtcagcctg cgggtacttt ttcgacccca tcagactcat atgttcaagg
    11581 taatgtgcca gcccctggta cgcctcggga tcttccagcg acccaacggg caccaccagc
    11641 gccgagagcg atttaactgc ctgcggatca gaaaccagca agaccaccat accgttatcc
    11701 agacgtatag cctgatactg gcggttatct ttatcacttt tacggatggt ttcctgaatc
    11761 ggctgccatc ccgtttctgc ctgacttaag ggtgcccaaa gggcaactaa caacaataat
    11821 gctttgaacc aggtgctgcg gggcattcac ggacctcata atcaacttaa ttttctgtcc
    11881 agattcaaca cgttaacgca aatcatctgc cagaatttaa tcttgtgctg cacgagtcag
    11941 cctatgttta tataaccatc agtccgtgac tggtgcgcat cataaagtaa gcggatagat
    12001 tgcgcaattt ttatacagca ctcatgactg attaaagcga aacagcggta acaggaaacg
    12061 ttgcgactgt tcaacgatgg cctccattgt ctctggtgtt aattgccgcc agagcctttg
    12121 ataccagata tcatcacctt cgccacgcac catcatgttg ccttcgtaag cctgaaggaa
    12181 tttcgtacgg gctttttgca acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc
    12241 ataacaggtt tttagccacg cgccgccact ttcaggtaac accagcaatg gcgcggacat
    12301 cccttcacga tacccctcaa tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag
    12361 cggcggaaaa cgccactcgc cgtctttgcg tagaaaaagg cgactttcac cattaccacc
    12421 gctggcacag tagacaaggt gttccagcca aagttgcatt ccctgcgcca cacttaataa
    12481 agagggacgc cagcgcaaca ggccatccgg ctgcacctgc ggcaaccagc cagttatctg
    12541 cacaccgttg caggcgagat caatttccat actctgcccc ggctggcgac aggcaatgac
    12601 tctgtcggca agctgctgca tctcctggca ctgtgtttcc cagaaaattt caccaaaagc
    12661 gccatacggt aaatcccctg ccgcccggaa gcggcggaac aagcgttcgg catcatcctg
    12721 ctcaaccagt gcattcaata actgctgatt gatttgataa cggctaagcc cttccagaat
    12781 aaatggctcg gtgtctggga tttcgctgtc ttcagtacgg aagttcacct gcaaacgcat
    12841 ctggaagaac gcccgcaccg gatgtgtcca gaatcgttgt agcgtttcca gcggcacggt
    12901 ttccggtaag gtaaacggca gcggctgaac aaattcagaa tgtgctttac cagcctggct
    12961 ggccgcaggt agccattcac gagcatagct ttgtcgttcg cctggctggt agttttgcgg
    13021 atcaaacggc atccgggtat ggaggcaagt aagatgcgct tttacccttg cctcgctttc
    13081 atcacagttg agcgcttcat cgtccggtag ataatgactt tgcccgatgt agtcgatcag
    13141 ttcctgcacc agtaccgacg ggaaacgttc actgttatcc tgaatggaac gcccgatata
    13201 gctgatatag agtttttgct gcgcggaaat taacgcttcc aggaacaggt agcggtcgtc
    13261 atcgcgacgg ctacggtcgc cacgcttcgg tttctggctc atcaggtcaa agcccaatgg
    13321 cgcaagctga cgtggataaa cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg
    13381 aattgaacgc attggcatca gagtacaaat gttaaccggt ccggcgagaa aacgctggct
    13441 gatacgttct tgatccagac gctgcgccag ttcatcacgc aatagtgaca gcggcaccgc
    13501 gtcgccatac tgcgcaccta aaccttcggc gataatcgcc tgccattgtt gttcgatcag
    13561 cgtcatcgcc gcttcggttt ccgcatccgg caggaagaag gcgttgagca tatcgcgaca
    13621 aaccggcaac cactcttcta gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag
    13681 ctgcattagc agtgaagcca gatgccccac cagttctgca attaagccgc tcgattcatc
    13741 ataaggtaga accgattgcc actcgccctg cgcgctctcc atcgcgtagc ccagcaacat
    13801 acgcgtcagg ccaaatcgcc aggtgtgttg tccggtggcg gggagttcca gctcgcgaac
    13861 gttgtcgtca tctatccccc aacgaatgcc ggattcgttg acccactggc gtaaataacg
    13921 cagcccttct tcggtgatgt caaaccgcgc cgccagcacc ggcacatcca gtaatgccag
    13981 cacgtcttcc gacacaaagc ggctgtctgg caacgataac aggctgataa acgcttccag
    14041 taccggatgc gactgccgcg cccgacggtc ggaaatggcg taaggcaggt aacgatccgc
    14101 aggtgcgcta ccaaacacag cctgaataaa cggactgtag ctgtcgatat cagccaccat
    14161 cacgatgatg tcgcgcggag taagtgtcgg gtcttcttcc agcatcgcca gcaggcgatc
    14221 gtgcaaaact tcaacttcac gctgcgggct atggcaaacg tggaaggtga tactgctatc
    14281 cagtggatca agcgggcgtt tgttatcgct acgggaaaac tcttcgatgt tcacaccagc
    14341 aacggcacgg ttttccagtt ccagaatgtc agactgaatg ttatgcagca ggttatctgg
    14401 cgtgacatcg acaaaggcgt ccagctcctg gctgctctcc aggtcagaaa ggagataaat
    14461 gtagtcgcgc ccaagcttac cccatgaagc cagcagcggg ttgccgacat cctgttcacc
    14521 atcgctgtta aagagctgcc cggcattttc gctatcacga aatagcggca attcgcgatc
    14581 ttcaacactg tgtcgacgct gacgggtcag cagtttcgcc agataagcag gatctttaat
    14641 atcgccccag taataacggc aggggttggt aaacaggaga tggatttcaa tatgtttacc
    14701 cagcgcctgg agcgcctgga gataaacagg cggtaacgcg gaaataccgc atataaagac
    14761 gcgcgaaggt aaccccggcg ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg
    14821 atagagattg gcgcggtgcc aacgcggttg tccgagttca tgggtatatt ccaccagcgc
    14881 cttccacaac ggcgcttgcc aggcctgtgc ttctcccagc ccttcaacca aatgtcctgt
    14941 ttcccactgt gccagccact ccgggcgata gaccagatac tggtcaaaca ggtccgccgc
    15001 ttttgaggaa agctggaaca gttttcgctt gtcgctatcg tcagccagat aatgccgcaa
    15061 cagggtaaag tcttcgcgat ccagcaattg cggcagcaga gtcatcagtt tccagctcat
    15121 gctctgtttg ttaaaggcgc tctctttggg gatctccggt aacacccgga cgaacatatc
    15181 ccagataaag ctcgctggca gcggaaaatc aatgtttgcc gcaataccaa acttttgcga
    15241 cagggtcatt tgcagccact gcgccatacc ggtactttgc accagaatca tctctggttc
    15301 gaaaggatcg tccagccgtt cgcgttcgac aataaactcc atcaacgctt ccagcacgtc
    15361 cagacgattg gaatggtaga cccttaacat agcggctcct gactactgac gattcgggca
    15421 atgcaggcgg gtcatctcgc cttctctgcc cccgggtgaa actagcgtaa cgctgatgct
    15481 gacacatccc gcctgcgatg tctgcattcg gttgacctgc cagttggcag gtggcgaaat
    15541 cgcgcgcagt tgcgtttgct gccaaccatg ccgccagagc tgttggtact ggtttctgct
    15601 ggcgagactg ttcattaatg ttcgctggat acccgataac gcagtgacaa tcatcaccat
    15661 cagcaccatc gcaaccatta cctccggcag gctaaagcct tgttgattct tcagggaagc
    15721 tgacataacg ccctctcttt cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca
    15781 atgtttccat caatgacttc gcctgttcgc cacaacgaaa cgccttcata accggcaacc
    15841 aataaggctt cattatctgc cagtaaacgc aaacaaaccc gggcatcggt tccagcgtac
    15901 tgcgagcact gaactgcggg ctgcgtctgc caggagtgca ttttccccca cgccagcgcc
    15961 gactgaacga tggcctggcg gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa
    16021 ctgcgatcct gctgactcat tccttgcaat agcaagctac ccaaaaccag cagcatcagg
    16081 accagagcca gtgacgaaac acctttttcg cggttcacag gttgaatcct gtcacgctat
    16141 agctggcatc caccacggtt tgcggttcag acttactggc agcacgcata ttaaccgtca
    16201 acaccggcga gaagccgctg acatcctgac gtacgacctg aaaagtgtcg ataatgatgg
    16261 catccggatt agtgacttta tcccagccct taccttcaca ggatgtcgca ccgcgtagcg
    16321 tttccagcac atgctccttc agacgaaatc caatctggtc agactctttt accggttcgc
    16381 gatcccagat accgttactg ttcgcatccc actgcacaat gatacagtca ccctgtccga
    16441 caatttccag cccttcgccg gtacagctgc catgacaata acccgccctc tggagatgct
    16501 tcgcgacggt aaatacccgc agccagattt catcttccag cgccagctta cgggtgctcg
    16561 ttaaactttc acgctgtaac gcaggcagaa agcgtgcagc ccccagcaac aatacgctac
    16621 tgatcgccat agcaatcaac acttccagca gagaaaaacc ttgctctttt acaggcatcc
    16681 ttctgtttct ccttgctgac aaagccggag tcttccccac ggcgaaacca ccagccacca
    16741 ctcgcccgtt gagtttttga agcgaatatg cccggcccat gcggtattgc gcaggccaaa
    16801 gaaagcaagc gaaggtgtca ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac
    16861 caatggtgaa ctgccatgac aggtattggc cccagcagcg gaactcacaa ggcaccataa
    16921 cgtcccctcc ctgataacgc tgatactgtg gtcgcggtta tgccagttgg catcttcacg
    16981 taaatagagc aaatagtccc gcgcctggct ggcggtttgc catagccgtt gcgactgctg
    17041 ccagtattgc cagccataga gtccacttgc gcttagcatg accaaaatca gcatcgcgac
    17101 cagcgtttca atcagcgtat aaccacgttg tgttttcatg ccggcagtat ggagcgagga
    17161 gaaaaaaaga cgagggccag tttctatttc ttcggcgcat cttccggact atttacgccg
    17221 ttgcaggacg ttgcaaaatt tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa
    17281 accgacgcac actggcgtcg gctctggcag gatgtttcgt aattagatag ccaccggcgc
    17341 tttaatgccc ggatgtggat cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa
    17401 gatggattcg ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa
    17461 ttgcagatga gtttgatcca tatgattgct gtacaggtgc gtgtcgcccc ccgtccagac
    17521 aaaatcgccc acttccagat cacactgctg cgccatcata tgcaccagta acgcgtagct
    17581 ggcaatgtta aacggcaggc cgaggaaaac gtcacaggag cgctgataaa gctggcaaga
    17641 gagtttgccg tctgccacat agaactggaa gaacgcatgg cacggtgcca gcgccatttt
    17701 atccagttcg cctacgttcc acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt
    17761 cagctggttc agtaccgtag tgatctggtc aatatgacga ccatccggcg ttggccaggc
    17821 gcgccactgt ttaccataca ctggcccgag gtcgccgttt tcatcggccc attcgtccca
    17881 gatggtgaca ttgttttcgt gtagataagc aatgttagtg tcgccctgca gaaaccacag
    17941 cagttcatgg atgatggaac gcaggtggca acgtttagtt gtcaccagcg ggaatccatc
    18001 ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc
    18061 gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt
    18121 tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc
    18181 cgcgacaatc atcgggatgg aaagaatttg tcccatgctg atgtactgca cccaggcacc
    18241 agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtaacc
    18301 aatcaggaac aaacctgaga cagctcccat tgggcgcggt ttacgaatat acaggttgag
    18361 gataataaac agcactacac cttccagcaa cagctcgtaa agctgtgatg ggtggcgcgg
    18421 cagcacaccg taagtatcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat
    18481 atcttctgta cgggagccag ggaacagcat ggcaaacggg aagttcgggt caacgcggcc
    18541 ccacaattca ccgttaataa agttgcccag acgcccggca ccaagaccaa acggaatgag
    18601 tggtgcgata aaatcagaga cctggaagaa ggaacgttta gtacggcggg cgaagataat
    18661 catcaccacg ataacgccaa tcaggccgcc gtggaaggac atgccgccgt cccagacacg
    18721 gaacagatac agcggatcgg ccataaactg cgggaagttg tagaacagaa cataaccaat
    18781 acgtccaccg aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt
    18841 ggtccagcca ctgcccggac gattcgcccg tcgtgttgcc agccacattg caaaaatgaa
    18901 acccaccaga tacatcaggc cgtaccagtg aagcgccacg ggtcctattg agaaaatgac
    18961 cggatcaaac tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt
    19021 atttcgctga aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg
    19081 tgcgaataat agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata
    19141 tgatccgcgc tataaccctc cgcgaatcag cccgcccatg ccacgacgct ccataaaggc
    19201 tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt
    19261 ttctgcttcg gcataatcaa tgcgccgcag caggtatttt acccgcgcta cagaacgtcc
    19321 gttcatagac agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc
    19381 cgccatttca ccgcacaaac ggagatcgat tccatgtatt tccgcttccc gggcgatcat
    19441 cgccagagct cgtaacattg caggatgaag actgtcataa atgttcgcca cccgggtatt
    19501 gttgcgatca acggccagaa tgtattgagt cagatcgttg gtgccaacag agatgaaatc
    19561 gacccgcttt gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc
    19621 gatacgtggt ttgggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc
    19681 aatcaggcgg cgtgcttcat caacttcatc gaggcttgtg accatcggca acagaatatt
    19741 caggttgccc gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc
    19801 cggctgatcg agcgtaatgc gaatcccacg ccaacccagg catggattct cttcgctgat
    19861 cggcatgtaa ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt
    19921 atcattgaac atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacgggaa
    19981 accactttgc agcatgaatg ggatttcagt gcgataaaga ccgatgccat caatacggct
    20041 gcccagtttt tcttcatgtt ccgggcttaa accagcattg agcatgactt ttatacgctc
    20101 accgcttttt aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat
    20161 ctcttcacta attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa
    20221 ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc agcaccgaag gctgaatatc
    20281 cgcgcccatc acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc
    20341 tgcgccatct cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagagttgt
    20401 cgctgacagt tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt
    20461 agcgtcatca agatgaaaca gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc
    20521 tttgagatag ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt
    20581 taccgcccac tctgccaccg agcctttatc aacctcggca aacaattcgc gacgcagccg
    20641 ggtgtccgaa agtaggtgcg agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc
    20701 ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt
    20761 cagtcgttcg cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa
    20821 aggtaacgtg gcatcctgcc agccttcggc aatcgccaca ccaggtgctg ccggtaatgc
    20881 gcggattcgc gtctggcgat attgcccaaa cagggcagtc aactgcgact gggaaagaat
    20941 agctgccatc tgggtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg
    21001 caactctcgt tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac
    21061 gcctaaaaac gcgcggaaac gttcttcttt tacggagggg atgtatttga agctggggtg
    21121 cttttgcgca tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc
    21181 ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat
    21241 caggtagtaa caacgtcgat catgatcggc caggtagacc gaacagacct cggtatccat
    21301 cgcaagacag atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc
    21361 taccttttcg actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt
    21421 acgtcgataa gcagatgcgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac
    21481 actcgcgaac tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct
    21541 gaccggatac cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg
    21601 catattgatt tctgcatcgc cgcttaccag ctgcaagaga aaccattttt gtttttggcc
    21661 gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca
    21721 gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa
    21781 ttcacggtac atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg
    21841 ccaggagtgc tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac
    21901 gatacctacg tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc
    21961 tttatatacg aatgattgtt tcatactcca ggaagacggt aaaccactct ctgcagggca
    22021 ttacacacta ataacaattg aataactcac agttatgtgc agagttataa acagaggaag
    22081 gggtggatag ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat
    22141 tactacggga aaacccggag cattctgaat aagcccgata tagacatttt aaaatattca
    22201 ttattagtca atgcattaca tgtaatttga gtaatgaaaa agagtaattc gtgacccagc
    22261 tcacacctct catttacggg ttggcgaaag atcaaccaat gccgtattta tccacagaat
    22321 gtgccactaa gttaagcact gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg
    22381 taaatggaaa cagtagtgga ggtttttcac agttatccca gctttctgtg gataacatgg
    22441 tgtaagatcc tgtttatttt cagtgaccag atttggaaaa cctattgcag tgttgcgcaa
    22501 ctcgattacc ggcagcctaa aaagcaatat aaatcagaga attgaacaac acatatggaa
    22561 aaagttacac tgcgaatatt cggcacataa ttgctgtttg ttttttaacc aaggtatcat
    22621 gacatgtccc aacctcgccc actgctctct cctcccgaaa ctgaagaaca gttgttagcg
    22681 caagcacagc aactttctgg ttatacattg ggagaactgg cggcacttgc cgggctggtt
    22741 acgccagaga atttaaaacg cgataagggc tggattggcg tgttactgga gatctggcta
    22801 ggtgccagcg cagggagtaa acctgagcaa gattttgctg ctctgggcgt ggaacttaaa
    22861 actatccctg tggatagtct tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg
    22921 ttaacgggta atagcggggt gacctgggaa accagccatg tgcgccacaa gctcaaacgc
    22981 gtactgtgga taccggttga aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga
    23041 tcaccgttgc tgtggagccc gaatgaagag gaagaccgac agctacgcga agactgggaa
    23101 gaattaatgg atatgattgt tctcggtcag gttgagcgga tcaccgctcg tcacggggaa
    23161 tatttacaga taagaccgaa agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc
    23221 cggggcgaac ggattctgac gctgccacgc ggcttttatt tgaagaagaa tttcaccagt
    23281 gcactactgg cccgtcattt tctgatccag tagccatcgc tttgacctgc cgctttccgg
    23341 gcatataatt accgcttcat ttttttggca gggcttttta gatgttattt gcatggataa
    23401 ccgatcctaa cgcctggctt gcgctcggta cgctgacgct gctggagatc gttcttggga
    23461 tcgacaatat tattttcctt tctctggtgg tggcaaagct tcccacagca caacgtgctc
    23521 atgcgcgccg tctggggttg gcgggagcca tggttatgcg tctggcgctg ctggcatcaa
    23581 tcgcctgggt tacgcgcctg acaaatccgc tttttacaat attcagtcag gaaatttctg
    23641 cccgtgattt gattctgctt ctgggtggct tgttccttat ctggaaagcc agcaaggaaa
    23701 tccacgaatc cattgaaggt gaagaagaag ggctgaaaac acgcgtttca tcattcctcg
    23761 gcgctatcgt gcagattatg ctgctggata ttatctttag cctcgactcg gtgattacgg
    23821 ctgtgggtct gtcagatcac ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg
    23881 tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga acgccatcct tcggtaaaaa
    23941 tgctggcgct ctctttcctg attctggtgg gctttaccct gattctggaa agtttcgaca
    24001 tccacgtacc gaaaggttac atctacttcg cgatgttctt ctctattgcg gttgaaagcc
    24061 tcaacttgat tcgcaacaaa aagaatccgc tctgatactc cgtacgctct cctgcgggag
    24121 ggcgtactca ctccttttac cctcacagtt taagattttc ctgctttcaa gatatataac
    24181 gtcggtttat aaacagacta ttatcatagg taggccaaat gaggataacc ggatgaaaaa
    24241 atgggcagta ataatttctg cagtcggact ggcgtttgct gtttccgggt gttccagtga
    24301 ttacgtcatg gcgaccaaag atggccgtat gattttgacc gatggaaaac ctgaaattga
    24361 tgatgatacc gggctggtga gttatcacga tcagcaaggt aacgcgatgc aaattaaccg
    24421 tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg tcagcatccg gctggcctta
    24481 agatttttct ctttcccttt tcccttccct ctgccatttt tatattcctt atgtcgtgat
    24541 tataaaaagg aaacggctat gcaatatcac cgtatacccc acagttcgct ggaagtcagc
    24601 acgctggggc ttggcacgat gacgtttggt gaacagaaca gcgaagccga cgcccacgca
    24661 caactcgact atgccgtcgc tcagggcatt aaccttatcg acgttgccga aatgtaccca
    24721 gtacctccgc gccccgaaac gcaagggtta accgaaacct acgtcggcaa ctggctggcg
    24781 aaacatggca gccgcgaaaa gttaattatc gcctccaaag tgagcggacc gtcgcgcaat
    24841 aatgacaagg gcatccgccc ggatcaggcg ctggatcgga agaatatccg cgaagcgctg
    24901 catgacagcc tcaagcgcct acagactgat tacctcgatc tttatcaggt gcactggccg
    24961 cagcgcccga ccaactgctt cggcaaactc ggttatagct ggacggattc tgcgcctgcg
    25021 gtttcgctgc tggatacgct ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt
    25081 tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc gctacctgca tctggcggac
    25141 aaacacgatc tgccgcgtat tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt
    25201 tttgaagtag gtctggcaga agtcagccag tatgaagggg tcgaactgct ggcctattcg
    25261 tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca
    25321 cgtaatacgc tctttagtcg gttcacccgc tatagcggtg agcaaacgca aaaagccgtc
    25381 gcggcgtatg ttgatatcgc cagacgtcat ggcctggacc ctgctcagat ggcgctcgcg
    25441 tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg gcgcaaccac gatggatcag
    25501 ctgaaaacta acatcgaaag tttgcatctg gagttaagcg aagacgtatt agctgaaatt
    25561 gaagcggtgc atcaggttta tacttatccg gcaccataag attaacgccg gttaaaaccg
    25621 gcgttaaata ttaatgacgg cgctgccaga tccacagcgc cgttattgcc agcgcaaaca
    25681 gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc tatcattacc gccagcgagt
    25741 aaatgcccag catcaacaac atggcgctgt tttcgccaag gttttgtact gcaatcgcat
    25801 tccccgcccc gacgcttttt ttaccccgct cctgtagcaa cgcattgagc ggaacgacaa
    25861 aaaagccccc catcacgcca atcagcatca acaaggcata ggctggcagc agctcgtgtt
    25921 gcagggaaaa aatcagtacc accacgccaa tcaaaatccc ggctggcata cagcgtgaca
    25981 cggtttccag cgtaactaac ttcgctgccg cacctgcgcc aaccacgata ccaatcgcta
    26041 ccatcgcgtt gagataggtg ggcgtagcgt tatcggtaat gcccagcgcc accggtaccc
    26101 acagcaccaa caggaaacgc agcgtgacac ccgctcccca gaataaactg gtgcccacca
    26161 gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca ggcattcagg aaactgcggg
    26221 tcatgttgat gagattccag gactgccccg gacgcgccgc cgccagtttg ggaatgtaga
    26281 tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc ggccagggcg acgaggacat
    26341 gccagtcagc cagcacacca ccggctacgg aaccgagcaa aatcgccgct atggtagaag
    26401 cttccattaa accgttagct ttcactaact tactacccgt ggttaattcg ccgagaatac
    26461 cgtatttcgc cggtgaatag gctgcagcac caacacccac cagcgtatag ccgagaaacg
    26521 gattgatacc aaagcagata ctggctgcgc ccagcagctt caggccgttg gcaaacatca
    26581 tcacccggcc tttggcgaag ctatccgcca cctgcccgac aaacggcgca aaaagaatgt
    26641 aagcacctac aaacaccatt tgcaggatgg gctggctcca ctccggatag aactgcgctt
    26701 tcagtaacgc cagagtggca aacagtaggg cattatcgcc aaacgcagag agaaactgcg
    26761 ccacgataac cgctttcatc cccttcgacc acaacgaagt gttagtgtgc actgactcac
    26821 tcatcgtgtt gttccgcttc gtctacccag cttttcaacg tgacaaagtc aggtttgccg
    26881 ctgccaagta atggcatctg tttcagatag cgaatatcgc gcggtacagc aagctccggc
    26941 acgccgtgct cgcgggcata ctgttgcaac ttatcgcgcg tcagttcgtt atctgtggtg
    27001 aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa tcgcagtggc atggacttta
    27061 tctggcgaaa caccaagtgc cagttgttcc accatttcca gcgacaccat ttcgcctgca
    27121 attttggcaa agcgttttgc gcggccctga atctgcacaa agccctgctc gtcaaaacgc
    27181 acaatatcgc cagtgtcata ccagccgcgc tccatttcgc cgcgaacatt ctcggcggtg
    27241 ggcacttcca gtacacctgg cttctccacc cgcagatagc cgttcattat gttcggccct
    27301 ttcagttgca ggcgtccgcc ctcttcgata ccaggaaccg acaacaggcg cgcatccatt
    27361 cctggtagaa tacgccctac cgtaccgggt ttcgccgcca tcggtacgtt gatagaaacg
    27421 acaggcgcgc actcggttac accatagcct tcaaggatgc gcaggccaaa tttatcctgc
    27481 caaagctgtt tggtactttc ttgtagtttt tctgcgcctg ccaccacata gcgtagacga
    27541 tagaagtcat acgggttggc gaaacgcgcg tagtgaccga ggaaagtcga ggtaccgaac
    27601 aacacggtgc aactgcggtc atacaccaac tccggcacaa tgcggtaatg cagcgggctt
    27661 ggataaagga acacttctgc acctgtaagc agtggcgtaa acaggcctac cgtcagccca
    27721 aaggagtgaa acagcggtaa tgccgacata aagcgatcgt tggtggtgaa gtcggcaatc
    27781 gttttaatct gctcgacgtt cgccagaatg cttttatggc tatggacgac gccttttgga
    27841 tggccttcag aaccggaggt aaagaggatc agcgcctctt cttccggctg ctgttttacc
    27901 tgcgccaggc gcggcatcag caaatgagcg aagatccata ctttgtcggc agtggtgaca
    27961 tctgctttta aatcttccag atagacccag cgcacctgag taagttgctc cggcagatgc
    28021 cagagtttgc ctttatcgag aaactggcgg gaagtgaaga tggttttgat ttcagccgcc
    28081 gtaatagcac tggtcagccc ttttacccca gcagtgtagt tcatcattgc gggaatgcgg
    28141 cgacgcgcga tggccccaaa aatcactgcc gcactgatgc ctgcattggg cagcattaag
    28201 ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc gtccaacaaa cagcgttttc
    28261 gtaagcaatt tgcgatagga gtctggggta aagttgacgt cttcgacaca tttcttcccg
    28321 gctccgaagc ggtacattgc actcagtaaa gattcgtaca gagtttcacg cgggcgcacc
    28381 gccatacgcg cttccatcat tatttgatgc agcatttcgc cagcgatttt gcgacggtca
    28441 cgggcacgcg gcgcatccgg catcgccacc tgcgttggtg gcaaaatatg cagagtaatt
    28501 tgcgggaaca agcgacgttt aaccagacct ttcaggcggc tgaagtgcgt aagttccgcc
    28561 ccttcaatac gcacaggaat aaccgttgca ccagacttcg ccgcgacaaa acccgcgcca
    28621 tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc cttcagggaa aatcaccact
    28681 ggtcggccct gttccaccag acgtaccaga tgtttaatag ccataggttg cgtcgggtcg
    28741 agaggaacaa agtcgataaa tgatttcagc caacgcatat accactgttg gcttattgag
    28801 gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc caagcaaaat gccatcaata
    28861 aaagagacgt gattaggcgt aattagaacg cgctcgccct tcagtgcctg ggtgtcaccc
    28921 gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa aaaagctaaa aagcattcca
    28981 actccctttg ctctgattca gtaaaagcga atggagggag attacacgag ataaagaacg
    29041 cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat ccgcgcaggt tggtgcaaga
    29101 gacagggtac gaagagcgta ccgaataatc tcaccaatca atacctctgg gatcttgatt
    29161 gtggtctgca cgacgactct tcgccagcga gaaaacgcaa aggaatgaag ggaaatgcaa
    29221 cgaggtgtgt aaattgtcgg ttactgttac agattgatga ccggcaaaaa aaaacctgcg
    29281 catctgcgca ggctggtgta attcatgtgc tcaacccgaa gttgacttca cctatcaata
    29341 cctctgggat caccacttta gcaacctgaa gccaaacgcc accagcggtc aatcgcaaca
    29401 gcgtttcgca aagtgtaacc aaaggtttgc aatctctttt tttgtcttac ttatttgcgt
    29461 ttggctcgca ttcccacgat gaaaacacgc caccccttga accaacgggc gttttccgta
    29521 acactgaaag aatgtaagcg tttacctact aaggtatttt catggcgacc ataaaggatg
    29581 tagcccgact ggcaggcgtt tcagtcgcca ccgtttcccg cgtcattaat aattcaccta
    29641 aagccagtga agcttcccgg ctggctgtgc atagtgcaat ggagtctctt agctatcacc
    29701 cgaacgccaa cgcccgtgcg ctggcgcagc agaccactga aacggtcggt ctgatcgttg
    29761 gtgatgtttc cgatccgttt ttcggtgcaa tggtgaaagc ggtcgaacag gtggcttatc
    29821 acaccggtaa tttcttattg attggcaacg gttaccacaa cgaacaaaaa gagcgtcagg
    29881 ctattgagca actgatccgc catcgctgtg ctgcgttggt cgtccatgcc aaaatgatcc
    29941 cggatgccga tttagcctca ttaatgaaac aaatgcccgg tatggtgctg atcaaccgta
    30001 tcctgcctgg ctttgaaaac cgttgtattg ctcttgacga tcgttacggt gcctggctgg
    30061 caacgcgtca tttaattcag caaggtcata cccgcattgg ttatctgtgc tctaaccact
    30121 ctatttctga cgccgaagat cgtctgcaag ggtattacga tgcccttgct gaaagtggta
    30181 ttgcggccaa tgaccggctg gtgacatttg gcgaaccaga cgaaagcggc ggcgaacagg
    30241 caatgaccga gcttttggga cgaggaagaa atttcactgc ggtagcctgt tataacgatt
    30301 caatggcggc gggtgcgatg ggcgttctca atgataatgg tattgatgta ccgggtgaga
    30361 tttcgttaat tggctttgat gatgtgctgg tgtcacgcta tgtgcgtccg cgcctgacca
    30421 ccgtgcgtta cccaatcgtg acgatggcga cccaggctgc cgaactggct ttggcgctgg
    30481 cggataatcg ccctctcccg gaaatcacta atgtctttag tccgacgctg gtacgtcgtc
    30541 attcagtgtc aactccgtcg ctggaggcaa gtcatcatgc aaccagcgac taaccgcagt
    30601 taaagcaatt ccagcgccag taattcttcg atggtctggc gacggcgaat caaccgcgcc
    30661 tgaccattat caaacagaac ttctggtaac agcggacggc tattgtagtt ggatgacatt
    30721 gatgcgccat atgcccctgt atcatgcagt accagataat cacccgcctt cacttccggc
    30781 aaggcgcggg tttcaacatt tcccccttcc tgctgggtaa agacatcgcc cgattcacat
    30841 aatggcccgg cgacgacggt ttccaccgtt ggcgcgtgtt ccagagaacg accatcagct
    30901 gccagggcac tgatatggtg gtaactaccg tacattgccg ggcgcatcag atcgttgaac
    30961 ccggcatcaa ccagcacaaa gtggcggcta cccatttgtt tgacgctccg cacctgagta
    31021 attaacacgc cagactgcgc taccagaaag cgacccggtt caatttccag tttcacaggg
    31081 tggcccaaat ggcgagcgat ttgctcacgc gcggcattcc acagaccata ataatgttcg
    31141 gtatcaaccg cctcttcacc ctgttgataa ggaatagaaa gcccaccgcc cgcagaaata
    31201 gcctgtaaat cctgaccgaa ttcgatgacc tgacgtacca tagcaccaca cacctgttcc
    31261 agatgggcat aatcaacgcc agaaccaatg tgcatgtgaa tgccgaccag ctgcaaatga
    31321 tgacgttgta tcacgtccag tgcggcgggc agatcggtgt accagatacc gtgcttgctg
    31381 ttttcgccac cggtattggt tttttggcta tgtccgtgac caaaccccgg gttaacgcgc
    31441 agccataccc gatgccctgg cgaaacctgg cccagttggt cgagcatatc aacagaaccc
    31501 gcattcaccg gaatttgcaa ttcactgacg cgttcaagcg tcgcctgatc gataacatct
    31561 gccgtaaaaa caatatcatc ggggtgcgtt tgcggattgt aacccgccgc caacgcacgc
    31621 tctatttcgc ctaacgagac ggaatccact ttcacgccct gctcacgcat taatcgcaaa
    31681 atatgaatat tggaacaggc tttctgtgca aagcgcacca catcaaactg tttcagcgct
    31741 gcaatctgcc gacgaataat ttgcgcatcg tagacccaca ccgggcagcc aaattcagcg
    31801 ggcaaacgca gcagattttc ggcggtgaga tcggtatcgg tgctgaacag tgaatgtggc
    31861 ataacaaact ccagatgagt gcttttttat gattacgcca catcataaaa agaataaaaa
    31921 atatcgattt atgtcgagtc tatgcaaaaa tgatatggat taccggattg cgagagagcg
    31981 ctaatggccg ccgttaactt acgtcatatt gaaatttttc atgcggtaat gaccgccgga
    32041 agcctgactg aggcagcaca cctgctacac acctcacagc caaccgtcag ccgcgaactg
    32101 gcgcgctttg agaaggtgat cgggctaaaa ttgtttgagc gcgtacgtgg acgattacat
    32161 cctaccgtgc aaggactgcg tctgtttgaa gaagtgcaac gatcctggta cggactggat
    32221 cgcattgtca gcgccgcaga aagtctgcgc gagtttcgcc agggagaact gtctattgcc
    32281 tgcctgccgg tcttttcgca atctttttta ccgcagctcc tgcaaccctt tctggcacgt
    32341 tatcccgatg tcagcttaaa tatcgtgccc caggaatcac cgctacttga agagtggctc
    32401 tcggcccagc gtcatgattt aggactcact gagacactcc atacgcctgc gggaacagaa
    32461 cgtaccgaat tactctcttt agatgaagtg tgtgtgttac ctccgggtca tccgctggcg
    32521 gtaaaaaagg tattaacgcc ggatgatttt cagggtgaga actacatcag cctttcccgt
    32581 actgacagct atcgccagtt gctggatcag ctatttactg aacatcaggt taaacgacgc
    32641 atgatcgtag aaacccacag cgccgcgtca gtctgcgcaa tggtacgggc gggggtaggt
    32701 gtttcggtgg ttaacccgct caccgcactg gattatgcgg caagcggttt agtggtacgg
    32761 cggttcagta ttgcggttcc gttcaccgtc agcctgatcc gccccctgca ccgcccgtca
    32821 tcagcgctgg ttcaggcgtt tagtgagcat ttacaagcgg ggttaccgaa actggtcact
    32881 tctcttgacg cgattttgtc gtcagctacg acagcataaa agcgacagca tcctcggcat
    32941 ggatcgccgc agtatcaaac acaggcaaaa cactgcgctc ttctggaacc aataaaccaa
    33001 tttctgtgca gccaaaaatg acaccctgcg ccccctgttc tgcaaggcga gcaatcactt
    33061 gcgcataata agcgcgtgac gcttcggtaa attgccccag acacagttct tcaaaaataa
    33121 tctgattaat tttcgcccgt tcatccgctt caggaataag acaattgatg gaaaattgtt
    33181 ccgtcagccg cccgcgataa aaatcctgtt ccatggtgta acgcgtaccc agcagcgcca
    33241 cacgagtcat tccggccccg gtaattgcac gtccggtggc atccgcaatg tgtaagaaag
    33301 gcagagtgca acgtgactca atggcatccg ccactttatg catcgtattg gtacataaca
    33361 caataccttc tgcgcccgcc cgctgtaagc caagcgccgc ctcagccaga atgtccccgg
    33421 ttttatccca ttccccgcga cgctggcact cttctatttc atgaaaatcg acgctatgta
    33481 gcagcacttg cgcagagtga agcccaccaa gccgctgttt aatgccttca tttatcagac
    33541 gatagtaagg aatggtggat tcccagctca ttcctcccag caaaccaatt gttttcattc
    33601 ctctctcctg atgtgtgtta ccgcaacaag gctgaataag gaacgagaca aacgcctcaa
    33661 cggccaggtg cccgatctct attaacgaaa aaagggccgg atgtacagca catccggccc
    33721 gtgaaatcag acgccgatat ttctcaactt ctcgcctgcc atcagtttgc gttcgatatg
    33781 ttccagcgtg acatttttgg tttccggaat gagccagaaa gtgatgccca caaacgcaat
    33841 gttcagcgca gtgtagagcc agaacgtacc ggcagcgcca atgctatcaa gcagtgtcag
    33901 gaaggtcgcg ccgataatca tattcgacac ccagtttgtc gtcgtcgaac aggtaatacc
    33961 gaaatcgcgg catttcagcg gctgaatttc agagcacagg atccacacca ctggcgcggc
    34021 gctcatcgca taaccggcaa tacacatcat cgtcatgcca acagagagcc aggacaagcc
    34081 actggaagcc gtaccgttat caaactgcat caggcaatag cccagcacca gagtgcctaa
    34141 cgccatcacg ctgaaaccaa ttttcagagc cggtttacga cctgctttat ctaccgtaaa
    34201 gaccgcaatg aaggtcgcga acataaaggt cagtcccacg accagagtcg caatcatctg
    34261 ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc ggcgcatagt acatgatgat
    34321 gttcataccg gtaaactgct gcatcgcctg caacaacata ccgagaaaca cagcacgacg
    34381 gacgttacgg ttgatcttaa acagtgccca accgccttgt tttaacttca ggctttcacg
    34441 aatttcgttg agttcttctc gcgctttttc cgacgtatcg cgcagcatac gcaacacttc
    34501 ttccgcctca atatgacgcc ccttttccgc cagccagcgc gggctatttg gcaggaagac
    34561 caccagaata atcagcagga ctgctggtaa agcaagaacc cccaacattg cgcgccagtt
    34621 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    34681 caactggtac atactgatca tcttaccgcg aacgttttca cttgccattt cagaaagata
    34741 cagaggagcg gtgtaagacg cgatcccgac agcaatgccc agcaccacac gagcggcgat
    34801 taacatctct acgctggtcg caaaagcgga ccctatagaa ccgagtacaa acaggatggc
    34861 ccccgccatc aggctgtatt tacgacccag gcggaacgac agccaaccat taaacagcgc
    34921 accaattgct gcgccgagca tcatgctact aaccacccat tcctgcaaac gactggtcag
    34981 cacaaagtga tcggtaatga acggcaacgc tccggcgatt acgccgatat caagaccaaa
    35041 taacaatcct gcgaccgcag cagctaccga aacaaacata ttcatacgcc gcgtatcccg
    35101 caaagaacgt ggcgttaaag cagattccgt attgatagta accatttttt cctgccagca
    35161 gagagtaaga catagtgaaa aaatacgtga acaactcacg caggtgtcag gtcggaaaca
    35221 gcataaatat ggattaaatt gctgcgacat gtcgttatgt gatggatatt ccaattttca
    35281 aattaagttg aattattgag attattatta accacctaat tttacagcag ataaaattca
    35341 taaagttcat taattgataa ttaatatgga ttatttcata accatgatat ggattatgat
    35401 gatctacagg tataaaaaac cctgccatgc ggcagggtca taaaagtaag aagaatgaat
    35461 taacgcgcca gccaaccgcc atccacggca atggtataac cattcacata atctgaagcg
    35521 ctggaggcaa ggaacactat cggccccatc aggtcactcg gcagtcccca acgaccagct
    35581 ggaatgcggt cgagaatttc cgcgctacgt tgttcatctg cccgtagttg ttgagtattg
    35641 ttggtcgcca tgtaacccgg agctatcgca ttaacattaa tgttgtgttt agcccattcg
    35701 ttcgccatca atcgcgtcac acccatcacg ccgctttttg atgcggtata agaaggcaca
    35761 cggatcccgc cctggaagga gagcattgac gcgatattga taatcttgcc gccattgcct
    35821 tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga atacgctctt gatattcagg
    35881 ttcatgacat cgtcccagtc cttttcgctg aactcgagag catcttcgcg gcgaatcaat
    35941 ccggcgttat tcaccaggat atcaatatga ccaaactccg ctaccgcgcg atccagcagt
    36001 ccaggaatgc catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc
    36061 gccgtgacct gctcgatggt ttcagtcggt tcaacgatgt taatgccaac aatgtcacag
    36121 cccgcttgcg ccagccccaa cgccatcccc tggcccagcc cagtatcaca accagtgacg
    36181 accgcaactt taccttcgag agaaaatgca cttaaaatca taacaatacc ttattcgttt
    36241 atgcccacaa ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt
    36301 tttcaccgac catgccccag ataaaggtat aagctttggt tccgacgccg gaatgaatcg
    36361 accagctcgg ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg
    36421 gctgccccat catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt
    36481 ccatccggcg ctcgtgagtg tgacacggca tggtgttcca caagttacct ggagccagct
    36541 ccgtcagccc catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa
    36601 ttgtgcgacg gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg
    36661 tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg
    36721 gagtggcggt atcaatactg gcaaagacaa cttcttttgc acctttaccc acatacaggg
    36781 cgtcgcggtg accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa
    36841 tattgataac acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa
    36901 cttccccgcc aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt
    36961 caatgtggct gtaaaccatg gtgtactcat cggcgacaaa tacctcttca accaaaaatt
    37021 cattgcgcag cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta
    37081 cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac
    37141 tgtttcgttt tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat
    37201 aaaacaatgt ttcattttta agttaagaat taaaaaaagt gcagtgtcga tcacgaatat
    37261 gtcatgagaa tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat
    37321 tattcaacag attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg
    37381 tcacgttcaa tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct
    37441 ttgcgggcat tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa
    37501 gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc
    37561 cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata
    37621 agatcgacat cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc
    37681 ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc
    37741 agcacgggta aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg
    37801 ccatcgttta tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga
    37861 tttaaacggg ctaaaccttc tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca
    37921 accaacgtct gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg
    37981 gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga
    38041 cggctgatgc catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg
    38101 aaggcatccc acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt
    38161 gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg
    38221 gcgatgacaa tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta
    38281 agcccagaac cgcaaacgtc attaatagtg attgcagaaa cgctattagg aagaccacct
    38341 ttaatagccg attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga
    38401 attacttcat ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc
    38461 agactaccaa gttccacggc ggaatgaccc gctaacgcac cgcgaaagca gccgataggt
    38521 gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg
    38581 caatagtaaa tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc
    38641 acaaaggaaa tatgcctgag cagtagtcag agtcataact ggcacgtaag gtttgcaacc
    38701 actaacccac caatagaggg gtagataggg cgttaatctc ccatacttaa cctggtttat
    38761 ggtaaattgc cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat
    38821 ttggtctaaa gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg
    38881 cacgctcttc cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc
    38941 tctggtcgcc tggcctttaa catattggcc acataaagcc ttatgccagt tcatcctctc
    39001 atcgaaaaca tcagcaggtg aagggataac gggcgcagta acacactact atggcaagaa
    39061 gattggtaat ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata
    39121 tgcagtggca attaccaact cacttacgga acagctggca aagcatatgg ttattgatct
    39181 tcgcatccgt atgttggtga gtctgggtgt tgtattaatc ctgaatctca tttttctgat
    39241 gggacgccat gcaaccattc gggtaatggg ttttttggta ttcccattga ttgcctattt
    39301 cttatttctt tccatttacc ttgtcggtag ttggcaacct gatctattaa cgacccaggt
    39361 agagttcaat cagaataccc ttcaccagat atggatatcg attcccgtga tggttttcgc
    39421 ctttagccat acgcccatta tttctacgtt tgccatagac agacgtgaaa aatatggcga
    39481 acacgctatg gataaatgca aaaaaattat gaaagtcgct tatctcatca tctgcataag
    39541 tgtactgttc tttgtcttta gctgcctgct ttctattcca ccttcgtata ttgaagcggc
    39601 taaagaagaa ggggtcacca ttttatcggc gctttctatg ctgccgaacg ccccagcatg
    39661 gctgtcaatt tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac
    39721 gtactttggc gttattgaag gtgccacgga ggtcgtcaaa acaacactac agcaggttgg
    39781 tgtaaagaaa agtcgtgcat ttaaccgcgc actatcaatt atgttggtat cgctgatcac
    39841 cttcattgtt tgttgcatta acccgaacgc gatttcgatg atttacgcga tcagcggccc
    39901 gctcattgcc atgatacttt tcatcatgcc tacgttgtca acgtatctga tcccggcgct
    39961 taaaccctgg cgttccatcg gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc
    40021 ggtaatgttc tttagctgat atccattgtg aatggttcaa aattgctaca attttgaacc
    40081 attccagttt tcataatgtt cattcgttat tattccgcac tatcacttcc ctcatcatat
    40141 cctagtatta tcagttagtt aaacacacaa agagatctga acatcttaaa taagactatt
    40201 taagatgcat aacttagatt cgcaagatat cttccggtca ttcaaaaaca atttgcgata
    40261 tagatattct cataccatga gatagttatc tgaaccaccc tcaaccaggt aagattaaat
    40321 cagtgaaaca taatcatatt taataacgag aaatgcattt taaaatgtct taatcagaat
    40381 tatagcaaat acagattaat cgcaattttt cccagcaaaa ttacaaaatc atggtgtatt
    40441 gacatcatca atcaccaatg aaattatgca attatatacg gatagggagg ttcttaacat
    40501 ggggctttgt agtcgttata aaagtcttac atgcaatagt tgctcaatgc attgccaaat
    40561 aatgccagaa gagtcaccgc gtttgcagta ctgcgcaaac tcgtgttttt gtatgtggcc
    40621 cgaagaaagt tcatatttta atcgtggtgt ggtagaaggt atcttaacaa aaaaccacaa
    40681 tgcgagatta agcggatata tttttgtcga tttttcagta agttttttac gtctattcct
    40741 tgaaaaagac tggattgact atcttgcaag tactgacatg gggattgttt tagtcagcga
    40801 ccgcaatatg caatcgttag ctaattactg gcgaaaacac aactcagcaa tatctgctgt
    40861 catatataat gatgatggtc ttgatgttgc caatgaaaag atcagacaac tatttattgg
    40921 tcgttattta tcatttacca gaggtaacac attaactcag atggaattca ccataatggg
    40981 gtatatggtt tctggttata atccatatca gattgctgaa gttctggata tggatatccg
    41041 tagcatctac gcgtacaagc aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt
    41101 atttattcgt tcacattcgg tccaatattg atactcaacg accagccaga atcatactct
    41161 gtttatacgg gcatttattg aaatgtccgt attttataaa taggccgtcc cctcaagagt
    41221 aaacaccata gtatttaaaa ccatcacacg aaaaattcag aagcattacg aaatatgaat
    41281 ttacaaacaa tagtggcata aatgttaacc atgttaattt acgtaa
//
LOCUS       DAZFJF010000029        40490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFJF010000029 DAZFJF010000000
VERSION     DAZFJF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.029"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(440..2257)
                     /locus_tag="ACHKAU_001721"
     CDS             complement(440..2257)
                     /locus_tag="ACHKAU_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO5530807.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIQAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYLM
                     SSVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEDSEDWMPSCASVTNDDGKN"
     gene            complement(2254..2553)
                     /locus_tag="ACHKAU_001722"
     CDS             complement(2254..2553)
                     /locus_tag="ACHKAU_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5530808.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(2560..2880)
                     /locus_tag="ACHKAU_001723"
     CDS             complement(2560..2880)
                     /locus_tag="ACHKAU_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530809.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVSSIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(2873..3076)
                     /locus_tag="ACHKAU_001724"
     CDS             complement(2873..3076)
                     /locus_tag="ACHKAU_001724"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5530810.1"
                     /translation="MRNHTIHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPDGCVMVFAARIRQGVFHA"
     gene            3274..3579
                     /locus_tag="ACHKAU_001725"
     CDS             3274..3579
                     /locus_tag="ACHKAU_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5530811.1"
                     /translation="MATANKNAKSQLTTVRVPLDVMQGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENPLVSSLDALAKVEQIGIKAAEEIGQLVTVAREELQRRKTKEPE"
     gene            complement(3474..4022)
                     /locus_tag="ACHKAU_001726"
                     /pseudo
     CDS             complement(3474..4022)
                     /locus_tag="ACHKAU_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4075..4263)
                     /locus_tag="ACHKAU_001727"
     CDS             complement(4075..4263)
                     /locus_tag="ACHKAU_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5530812.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(4638..5867)
                     /locus_tag="ACHKAU_001728"
     CDS             complement(4638..5867)
                     /locus_tag="ACHKAU_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5530813.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLESYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGAVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWMERLGI
                     LAGTHENVTTLPVARRK"
     gene            5855..6037
                     /locus_tag="ACHKAU_001729"
     CDS             5855..6037
                     /locus_tag="ACHKAU_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530814.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTSKITNLLIIK
                     NIMHILKHYETAKNTK"
     gene            6044..6949
                     /gene="tqsA"
                     /locus_tag="ACHKAU_001730"
                     /pseudo
     CDS             6044..6949
                     /gene="tqsA"
                     /locus_tag="ACHKAU_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(6974..8362)
                     /gene="pntB"
                     /locus_tag="ACHKAU_001731"
     CDS             complement(6974..8362)
                     /gene="pntB"
                     /locus_tag="ACHKAU_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO5530815.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(8373..9905)
                     /gene="pntA"
                     /locus_tag="ACHKAU_001732"
     CDS             complement(8373..9905)
                     /gene="pntA"
                     /locus_tag="ACHKAU_001732"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO5530816.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            10429..11373
                     /gene="ydgH"
                     /locus_tag="ACHKAU_001733"
     CDS             10429..11373
                     /gene="ydgH"
                     /locus_tag="ACHKAU_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO5530817.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            11559..12941
                     /gene="ydgI"
                     /locus_tag="ACHKAU_001734"
     CDS             11559..12941
                     /gene="ydgI"
                     /locus_tag="ACHKAU_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5530818.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            12978..13700
                     /gene="folM"
                     /locus_tag="ACHKAU_001735"
     CDS             12978..13700
                     /gene="folM"
                     /locus_tag="ACHKAU_001735"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO5530819.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(13697..14032)
                     /gene="ydgC"
                     /locus_tag="ACHKAU_001736"
     CDS             complement(13697..14032)
                     /gene="ydgC"
                     /locus_tag="ACHKAU_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO5530820.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            14161..14880
                     /gene="rstA"
                     /locus_tag="ACHKAU_001737"
     CDS             14161..14880
                     /gene="rstA"
                     /locus_tag="ACHKAU_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO5530821.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            14884..16185
                     /gene="rstB"
                     /locus_tag="ACHKAU_001738"
     CDS             14884..16185
                     /gene="rstB"
                     /locus_tag="ACHKAU_001738"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO5530822.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            16261..17190
                     /gene="tus"
                     /locus_tag="ACHKAU_001739"
     CDS             16261..17190
                     /gene="tus"
                     /locus_tag="ACHKAU_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO5530823.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(17187..18590)
                     /gene="fumC"
                     /locus_tag="ACHKAU_001740"
     CDS             complement(17187..18590)
                     /gene="fumC"
                     /locus_tag="ACHKAU_001740"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO5530824.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     LNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(18733..20379)
                     /gene="fumB"
                     /locus_tag="ACHKAU_001741"
     CDS             complement(18733..20379)
                     /gene="fumB"
                     /locus_tag="ACHKAU_001741"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5530825.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            20578..21753
                     /gene="manA"
                     /locus_tag="ACHKAU_001742"
     CDS             20578..21753
                     /gene="manA"
                     /locus_tag="ACHKAU_001742"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO5530826.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            21854..23362
                     /gene="ydgA"
                     /locus_tag="ACHKAU_001743"
     CDS             21854..23362
                     /gene="ydgA"
                     /locus_tag="ACHKAU_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5530827.1"
                     /translation="MNKSLVAAGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(23407..24672)
                     /gene="uidC"
                     /locus_tag="ACHKAU_001744"
     CDS             complement(23407..24672)
                     /gene="uidC"
                     /locus_tag="ACHKAU_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO5530828.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(24711..26084)
                     /gene="uidB"
                     /locus_tag="ACHKAU_001745"
     CDS             complement(24711..26084)
                     /gene="uidB"
                     /locus_tag="ACHKAU_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO5530829.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(26081..27892)
                     /gene="uidA"
                     /locus_tag="ACHKAU_001746"
     CDS             complement(26081..27892)
                     /gene="uidA"
                     /locus_tag="ACHKAU_001746"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO5530830.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(28281..28871)
                     /gene="uidR"
                     /locus_tag="ACHKAU_001747"
     CDS             complement(28281..28871)
                     /gene="uidR"
                     /locus_tag="ACHKAU_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO5530831.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(29092..29859)
                     /gene="hdhA"
                     /locus_tag="ACHKAU_001748"
     CDS             complement(29092..29859)
                     /gene="hdhA"
                     /locus_tag="ACHKAU_001748"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO5530832.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(29971..30999)
                     /gene="malI"
                     /locus_tag="ACHKAU_001749"
     CDS             complement(29971..30999)
                     /gene="malI"
                     /locus_tag="ACHKAU_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO5530833.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            31174..32766
                     /gene="malX"
                     /locus_tag="ACHKAU_001750"
     CDS             31174..32766
                     /gene="malX"
                     /locus_tag="ACHKAU_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO5530834.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            32776..33948
                     /gene="malY"
                     /locus_tag="ACHKAU_001751"
     CDS             32776..33948
                     /gene="malY"
                     /locus_tag="ACHKAU_001751"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO5530835.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            34052..35053
                     /gene="add"
                     /locus_tag="ACHKAU_001752"
     CDS             34052..35053
                     /gene="add"
                     /locus_tag="ACHKAU_001752"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO5530836.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(35088..36128)
                     /gene="ydgJ"
                     /locus_tag="ACHKAU_001753"
     CDS             complement(35088..36128)
                     /gene="ydgJ"
                     /locus_tag="ACHKAU_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5530837.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            36371..36496
                     /gene="blr"
                     /locus_tag="ACHKAU_001754"
     CDS             36371..36496
                     /gene="blr"
                     /locus_tag="ACHKAU_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO5530838.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            36769..36984
                     /gene="ydgT"
                     /locus_tag="ACHKAU_001755"
     CDS             36769..36984
                     /gene="ydgT"
                     /locus_tag="ACHKAU_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO5530839.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            37070..37510
                     /gene="ydgK"
                     /locus_tag="ACHKAU_001756"
     CDS             37070..37510
                     /gene="ydgK"
                     /locus_tag="ACHKAU_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO5530840.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            37587..38168
                     /gene="rsxA"
                     /locus_tag="ACHKAU_001757"
     CDS             37587..38168
                     /gene="rsxA"
                     /locus_tag="ACHKAU_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO5530841.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            38168..38746
                     /gene="rsxB"
                     /locus_tag="ACHKAU_001758"
     CDS             38168..38746
                     /gene="rsxB"
                     /locus_tag="ACHKAU_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO5530842.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            38739..>40490
                     /gene="rsxC"
                     /locus_tag="ACHKAU_001759"
     CDS             38739..>40490
                     /gene="rsxC"
                     /locus_tag="ACHKAU_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO5530843.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQV"
BASE COUNT         9837 a        10515 c         9746 g        10392 t
ORIGIN      
        1 gctgtggtca tgaatgcgct tatcagttcg tgatgtgctt tcgcgtatcc ttccccggcg
       61 ttgcggtcga tgatttttat cgccaccctc agccagtgtt ctgtcaaatc aagggcgtga
      121 gattgtggtt ttttggcgtg cttcgttgtc acaggcttta cctcacagca ataaaataaa
      181 atttttgcat ttcaaccctt cacctgttca ccttttacaa ttttcccttt ttattcatga
      241 tgttaagggg tgaacagttt cacaaaaact attcaccaac tgttcaccac tgttcaccct
      301 taaagctcaa taaagaatca aaaaggtgaa cagtgaatag tttggtgaac agttcataaa
      361 taactgttca ccctataata tactgatata aaagacattt attgcaggct gaacagtggt
      421 gaacagttat tccataagtt taattttttc catcgtcatt tgtgaccgat gcacatgatg
      481 gcatccagtc ttctgaatcc tctgtcaggg tcacatttga acgcaaaccg tgcttcgttt
      541 tccgtttcat atactccctg ccatattccg ccattgcccc cggcatatct ttaccgaagc
      601 gcgtcagtgt tacaggttta ccgaatccgt gtgccctcat atatgccaga taggcgtgat
      661 aaagatacct gcgcggactg aacggaataa tttcggcatt acccactaac agaccatcac
      721 acattaccga cgacatgaga tagccgcaga agtccaccag cgaatcgccc tcgcgtttta
      781 tcaccagagc ttcttctgat ttctgctgct catacagcag gcgtttagct tcgtcctggt
      841 cagaaaaacg agtaagcagg tggcgaatca caaccgccag ctctccttct attttttctg
      901 ccagcatggg gtcgcgttcg ttttccggta caacctccga aaaattgaat atcacccgac
      961 gacgtgaaat ccccccgctg cggtcactga atgacatggc gttattgttc accgccaaca
     1021 ctaccgcctg aatgcgtgtt gagtaggggg ctttatgctt cgggtcgatt gccaccttgt
     1081 caccgcctgt aatggcctta attcctgcgc catcaccagc gtaacgggtc atatccggca
     1141 tgataatcag cgaaaagcca accactaacg cgcgttccct ggcatcttcc agcgccttca
     1201 tgcttgccga taccgtgttg gccttacccg ccagcatggt gcaaatctcc gccatcacgc
     1261 ttttaccact tccccctgga cctgttacct caatgaataa ctgccagtcg taccggttcg
     1321 ccagcaccat gaataatgca gccagtacgc gatccgcctt gcggtcattc tcagccaccg
     1381 aacggcgtaa ccacttccag aaattcggcg catgtgttgc cagcgtttcc ccctctgctg
     1441 gtgggctgaa aggtaattca ctggcaatta acaaccagtc gtttttgtta tgctcccgaa
     1501 aattacctgt tctggtatca aataccccgt tactgaatcc aatcaggtta cgggctgtat
     1561 tccccattac aggcaaactt aacttcatgg tatcgaccgc cgatttaatg gcgttctgcg
     1621 aatagctgat ctccgcatca atgaaaatct gtgccatagc tcgctgtaat tctttatcct
     1681 gtactggctc ccatacaacg ccgttgtaat ggtgaacagt gtcagagtca gcatgaatcg
     1741 ccagttcacc gccataatgt gccaggagaa cttcgccgcg ttgacttgct cccatctggt
     1801 taagcgccag tgatgaagcg ttatcgtctt ttacccgctc ttttttcttt acaggcagtt
     1861 caactacctt tttcttttcc gcctgctctg cccgttcacg ttccagatat tcgcgccagt
     1921 tctcccgttt ctggctgtgc attccttcag ggtaataatc agcatccctg acacctgccg
     1981 ctgccagttt ctgcccgatg gtattaacaa gccccggacg caataacccc gcctggtaga
     2041 gacgcacccg atagcgtccg tccggtacga tttgcagctt atccagttcg gcaagttgtt
     2101 gttctccaag ccagacagga ggcacgctgt cgccagccag tcgcccgtcc tgttcctgcc
     2161 actgcttcgc atgtgcccac gcatcattac ccgcaaaaat gatgacttcc gtcattttgt
     2221 cacgcggctg gtgttttaaa tttggcgctt ttttcatttc tgctctctcc acgcggcaat
     2281 catgtttttc agttcctgta gttttttatc aacatccata catgacacat ggttatttct
     2341 ggaaagcggg atttcccgcc tgaatctgct aataaagatc tccacgttca gcgaactatg
     2401 aaatgaatag ccatcacgaa taaaatacac acggtcaaac atcagttctt ttaccgttac
     2461 tctgttaccg ttcttatcca gataaatagc gccggggata attttggggt gtgcataacc
     2521 gctggcagtc aagccagata aatacgttct catgattatt tatctcagat ttgaatcagt
     2581 attcgctttc tctatggcat ttaatgcatc tgtggcattt tcaatggtgc accgtaacga
     2641 aatatcaaac tgcccaagca ttgccagtaa caagccaata ttacccatgt caatgcgcat
     2701 agccttttcg tcatattcct cattttctga cgcatgccac atcaggctac caattgacga
     2761 aacagccatt gatatattgt cagtagcccc atccgcagcg gaataaacct ttttagcaat
     2821 atcatgctca cagttaaaat gcggattaat caggtactgg taattggtca tgtcaggcat
     2881 ggaacacccc ctgacgaata cgggcggcga ataccatcac gcagccatca ggagattgct
     2941 gacgcgcttc ctgttcgctg gtggcctcga tggtaatcac gcgcggttgt gccgtgctca
     3001 gggcgataaa acgccagatg tatttattca ggttgtgcga gtcccgccct tgcgggtgta
     3061 tggtatgatt tctcatagct gcctcgatac tctcgttatc gttggtggtt agacgccccg
     3121 tatgtgtttc cggcactgcg gggcgttgct ctttgtattt caaaaatcat ttcggtgtgt
     3181 ttcatgttat gagcgcatga aacacacgtc aaggcttttt gtatttcttt ttttgtgtat
     3241 actgaaacac accgatgatt aggagtttca gaaatggcaa cagctaacaa aaacgcaaaa
     3301 tcacaactga caactgtcag agtcccacta gatgttatgc aagggatgga atccgttaag
     3361 ctggacggcg aaagcaatgc cggatttatc gtaaccgcca tgcgcggaga aatagcccgc
     3421 cgccaagcag aaggcagcgg agaaaatccc cttgtgtcgt cactggatgc cctggctaag
     3481 gtcgaacaaa tcggcatcaa agcagcggaa gaaatcgggc agcttgtcac cgttgcacgc
     3541 gaagaactcc agcgtcgcaa gaccaaagaa ccagagtaat caccatcagc gccgtggtgt
     3601 aaggtattac ggcgcattgc tatgcaggac aacacaatga ccgataaaga attgaccaaa
     3661 acattatcac cggcacggaa aagacggcgc agaaagatag agcatgaatc agaaagattc
     3721 gcgccatgtg cttttgccct tgagcaattc cttaaagagt acagggaaaa gcgctcattg
     3781 caggtatggc aacgaactga accagactga tagcattgcc caccagcctg atagcgacta
     3841 tcatttccgt tcttatgttt gggatcacac acacaaacgg cgcagcgggt tatctgttca
     3901 gaaaggcggc tccatttcgg gaccgctttt tttattcctg aaaaacccca attttgttgt
     3961 ttttcagttt caccagggcg aacgaatccc cgcccacgtt cgggcgcata ttcaatcttc
     4021 atggttatat ctctgtatta gtggatgtgt ggcggctgcg tgccgccagc gtgattaatg
     4081 aactgccttg cagctatcct tccaggccag aacctcggat aaagaccagc caacggaacg
     4141 accgccaagt ttacgacgtg atgggaattg tccggccttt tccaggcggt agcggcatga
     4201 gcggctaagg cctgttagct tttcgcattc tttttcacgt ataaaccgat cagtgcttaa
     4261 cactattgcc ccctttcgtt tcttaaagag ttatttcgtg ttctattgcg ttgggatgtg
     4321 tctgattgtg tcaggatgat tcagagttgg caaatgttga ggctgtatgg tttacagaaa
     4381 gaggaataat caggataaaa tcatttaaat tcatgttaat acaaaggcat aaaatattgt
     4441 ttaatgcctt ttttctcgct atttaaagag tgattcgcta gtgtataaaa aaccagtaac
     4501 acattaaaaa tcagatactt ataaatctgt acactttttc gcctcttgtt cgtagttgtt
     4561 ccgcattgtt gctcattgtt gcacgttgta tctgttcgca tatccagtat gcgcatactg
     4621 aaaaaatacg aaaaaaatta ttttcttctg gctactggta gcgtggttac gttttcatgt
     4681 gttcccgcca gtatccctaa ccgctccatc cacatatcca gcgcattgcg tttagcatcc
     4741 agataacggg aatgattata aactcgctgc attcctggca tctggtggcc tgtaagctgc
     4801 tccacgacat gcggatcaac gcctaaatcg ttcagcatgg ttgtaaaggt gcgccggatg
     4861 tcatgcagtg accagtgagg gtgattaagc ctcctgtgcg ctaatcttcc atactgcgat
     4921 acgctggcct cctgtttcac tgcccccagc aataagcccg tgtgcctgtt ctgctccacc
     4981 agctgcgtga cgaacggcag gatcgcttcc ggtatgggcc ggaatattgc gaccttcgtt
     5041 ttgctgtgct ccttcggaac ggtccatagc atttccgtaa aatcccactc cccgatctcc
     5101 gataacctca gttctaccgt tctggctccg aagacaatca ggaggcggat taacgcgacg
     5161 tagtaagggg aaaatatttt tttatccagc gcctgcaata actcgcccag ttctttggtg
     5221 cttaagacac gttcgcttat atccggtttt ttcccaacgt ccgccacgtt cagatcgtcc
     5281 agaacgttgc tgattgcata gcgccgccta cggcagaact taagcgcctg tttgcacgcc
     5341 tgtagcacga atccggcagt aacaggcgtt cgctttgcca cctggtcaaa acaggccagc
     5401 cagtgccgta gctcgcattt atccagcggc atagcaccaa tctgctgtat tacgtgatta
     5461 ttaagtcgcc ttttcagggc gatataatcc acgcggtttt cctttacgta agactcaagc
     5521 cagtaggtga gcgcatcgcc aaccgttacg ggctttaacg cttcctgtac ggtgtaattc
     5581 atctcatgac gtggattttt cccctcagcc agccatgcgc ggcactgggc ggctttttcc
     5641 ctggctgctt tcagactcag atcaggataa tttcccagct taatacgttc cggtcgtgtc
     5701 tctcttcccg ttcctgccct gtaagtgaaa taccaggtta atttccctga tgttaaatat
     5761 ttcacgctca gatttccgcc atcgctataa aacgtgtttt tctccgctgg cttaccatga
     5821 agtttcctta gcaatgtgtc gctcagtttg ttcattgctc tgctcacgtt ttaccccctc
     5881 tttacgaaaa agtgactaca ccagtgacta caccgatcgt tgcacaaagg gctacaacgt
     5941 gaaacaagct ggaacaagta aaataacaaa tctgttgata atcaaaaaca taatgcacat
     6001 cctgaaacat tatgaaacag caaaaaacac taaatgataa aatgctgtta ttcttaaccc
     6061 gctggtgcaa cacatggtcc gctggcgagt gccgcgtgta ctggcggtgt cgattttgat
     6121 gaccatcatc gtgatggcga tggtgttgct attagcttat ctgggttccg cgctcaacga
     6181 gttgacgcgg acgttaccgc aatatcgcaa ctctattatg acgccgctgc aagctcttga
     6241 gccgttgttg caacgcgtag ggattgacgt ctcagttgac cagctggcgc attacattga
     6301 tccgaacgcg gcgatgacgt tgctcaccaa cttattgacg cagttatcta atgccatgtc
     6361 atcaatattt ttattgctgc tgacggtgct gtttatgctg ctcgaagtgc cacaattgcc
     6421 cggaaaattt cagcaaatga tggcgcgtcc ggttgaaggg atggcggcga ttcaacgtgc
     6481 aattgacagt gtttctcatt atctggtgct gaaaacagcc atcagcatca tcaccggcct
     6541 ggtcgcctgg gcgatgctcg ccgcactcga tgttcgcttc gcttttgtct ggggattgct
     6601 ggcctttgcg cttaattaca tccctaatat tggttcagtt ctcgcggcaa tcccccctat
     6661 cgctcaggta ctggtgttta atggcttcta cgaagcgttg ctggtgctgg cgggatatct
     6721 gctgattaat ctggtcttcg gcaatattct ggagccgcgc atcatgggac gtgggctggg
     6781 gctttccaca ttggtggtat ttttgtcgtt gattttttgg ggatggttgt taggaccggt
     6841 gggtatgctg ctttccgtgc cgttaacaat tattgtcaaa attgcgcttg aacaaacagc
     6901 gggaggtcaa agcatcgccg ttctgttaag cgatctcaat aaagagtgac ggcctccgca
     6961 gaggccgtca gggttacaga gctttcagga ttgcatccac gctggctttg gcgtcaccaa
     7021 acagcatgtg ggtgttttcc ttgaagaaca gcgggttttg cacaccagca tagccagtgt
     7081 tcatcgaacg tttaaagaca atcacgttct gcgctttcca cacttccagc acaggcatac
     7141 cagcaatagg actcttcgga tcatcctgcg ccgccgggtt aaccgtatcg ttagcaccaa
     7201 tcaccagtac ggtatcggta tcagcaaagt catcgttgat ctcgtccatt tccagcacga
     7261 tgtcatacgg tacttttgct tcagccagca atacgttcat atgtccaggc aaacgccccg
     7321 caaccgggtg gataccgaaa cgcacgttga taccacgagc gcgcagtttc tcggtaattt
     7381 cagcgacagg atattgcgcc tgcgcgactg ccatgccgta ccccggagta atgatcactg
     7441 aatgggagtt tttcagcagt tccgctgtct cttctgcggt gatttcgcgg tgctcgccca
     7501 cttcctgatc atcgccagta gaagagccgt cggtgccgaa accacccgca ataacgctga
     7561 taaaggaacg gttcatcgcc ttacacataa tgtaagaaag gatagcgccc gaagaaccga
     7621 ccagcgcacc ggtcacaatc agcaggtcgt tgctgagcat aaagcccgca gccgcagccg
     7681 cccagccgga gtacgagttc agcatcgaca ccaccactgg catatctgca ccaccgatgg
     7741 aggcgactaa atgccagccg aataccagcg caatggcggt cattatcagc aatgccagca
     7801 cttgcaggcc gacgctatcc gtgcgaacaa atacaatcag cagcaggaag gaaacgacca
     7861 gagccgccag gttcattttg tgacggtttg gcagcatcaa cggtttagac gaaatcttgc
     7921 cacacagttt gccgaacgcc accaccgaac ccgtgaacgt taccgccccg atgaagatac
     7981 cgaggaacac ttccgtcagg tgaatattga ccagaatcgg tgccattccc gcgtcatgat
     8041 gcagataact gttaaagcca accagcactg ccgccagacc cacgaagcta tgcaggatcg
     8101 ccaccagttc tggcatttcg gtcatttcaa ctttcttcgc cagacggata ccaattgccc
     8161 caccaatgac catcgccagc aagatccagc caacattacc cgtatccggt ccaaaaatgg
     8221 ttgcgattaa cgcaatcgcc atcccggcga taccgaagtt gttaccctgg cgagacgttt
     8281 catgtttcga aagaccggcc agactgaaga taaacaggat cgcggcaaca atgtatgcag
     8341 ctgtaactaa tcctccagac atatgttacc ccttaatttt tgcggaacat tttcagcatg
     8401 cgctgagtca cggtgaagcc accgaaaata ttaatgctgg ctataagcac cgcgataaaa
     8461 ctaaggaagc taacccagcc gccctggcca atctgcaaca gtgctccgac aacaataatc
     8521 cctgaaatcg cgttggtgac cgacatcaac ggtgtatgca gcgcatgcga tacattccac
     8581 accacgtaat aaccgacaac gcaggccagc gcgaaaacgg tgaagtgccc aagaaactct
     8641 ttcggcgcaa cgcttgccag ccaaccgaaa aggatgattg ccagcgccat caatgcgtat
     8701 ttacgccacg gtgagcaggc acatttttcc tcagttttca cttccggtgc cgctttttgt
     8761 gccgcctgcg gctgagctga tacctgaatc ggcggtgccg gccaggtaat ttcgcccgca
     8821 cggatcacgg tcacgccgcg aatcaccaca tcatcaaaat caacagtgat attgccgtct
     8881 ttctctttgc acaacagttt cagcagatta acgaggttag taccgtaaag ctgtgaggat
     8941 tgcgtcggca gacggcccgg aagatcggta taaccaatca ctttgacacc attttccgta
     9001 gtgaagattt cacccggcac ggtgtattca cagttgccac cgttttgggc tgccaggtcg
     9061 acaatcacac tgcccgcctt catggagtca accatttcac gggtaattag cttcggcgct
     9121 ggtttgcctg gaataagcgc ggtggtgaca atgatatcga cctcttttgc ctgggcggca
     9181 aagagttcca tttccgcttt gatgaacgcg tccgacatca ctttggcata gccatcgccg
     9241 ctgcccgctt cctctttaaa atccagctcg aggaattccg cgcccatact ttgaacttgt
     9301 tctttcactt ccgggcgggt gtcgaatgca cgcacaatcg cgccgagact gtttgctgcg
     9361 ccaatggcgg ccagacctgc aacacccgca ccaatcacca tcacttttgc cggtggcact
     9421 ttcccggccg cagtaatttg cccggtaaag aagcgcccaa attcatgtgc cgcttcaaca
     9481 atggcgcgat aaccggcgat gttcgccatc gagcttagtg cgtccagcga ttgtgcgcgt
     9541 gagatacgcg gcacagaatc catcgccatc acggtcacgt tacgttccgc aagtttttgc
     9601 attaattccg gattctgcgc aggccagata aaactcacca gcgttgtccc aggattcagt
     9661 aacgcaattt catcatctaa cggcgcattg accttcagaa tgatctctga ctgccagacg
     9721 ctattccctt ctacaatttc agcgcccgct tgcacaaacg ctttatcgtc aaaacttgcc
     9781 agttgacccg cgccgctctc taccgcgacg gtaaaaccca gtttcagcag ctgttccact
     9841 gtttttggcg ttgctgcaac acgggtttca ttggttaacc gttctcttgg tatgccaatt
     9901 cgcatgatat tcccttccat cggttttatt gatgatggtt tgcctgtgtc aggagccaca
     9961 caagccgctc atgtacgagc taaatgttac tccgttaaaa taaattagta acaaacgccc
    10021 tataacgtac tgaaaattat gcctgtgatc tagcgccaaa aatcagtatt tcggcgtgaa
    10081 ctcgcaaaat attaacgatt cagccgtgat agtgggataa acaccttaga acgccggata
    10141 aagactgata attgtcttcg acggtcgggt aaaacgagac atcgccccgg cacgaatcac
    10201 tacttaacat taaattaact tatacaattc agttgcttca gtagtaatga tgctgatacg
    10261 gctgtttttt aagcatagac ggtcatttga gcaggattaa aattggctta aggaatgtga
    10321 tatgaaaaat gacgcagaca gttacaccgt ttaaatgcaa taatcagcca cgtttctcgt
    10381 taataacaat accagtacct ggtttgcgca aggcgaagga ttatttttat gaagcttaag
    10441 aacaccctcc tggcgtcggc actgctttct gctacggcat tctccgttaa cgcagcaaca
    10501 gaactgacac cggagcaagc ggcagcggtt aaaccttttg accgtgtagt ggttaccggt
    10561 cgttttaatg ctattggcga agcggtgaaa gccgtttctc gtcgcgcaga taaagaaggt
    10621 gccgcctctt tttatgttgt cgacacttct gattttggta acagcggtaa ctggcgtgtg
    10681 gtcgctgacc tctataaagc cgatgctgaa aaagcagaag aaacaagtaa tcgcgtaatt
    10741 aacggtgttg tcgaactgcc gaaagatcag gctgttctga ttgaaccgtt tgacacggtc
    10801 accgtccagg gcttctatcg tagccagcca gaagtcaatg atgccatcac caaagcagca
    10861 aaagcgaaag gtgcctactc tttctacatt gttcgtcaaa tcgatgccaa ccagggcggc
    10921 aaccagcgta ttactgcatt catctataaa aaagatgcta agaaacgtat cgtccagagc
    10981 ccggatgtga tcccggcaga ttccgaagca ggacgtgcag ctctggctgc cggtggcgaa
    11041 gccgcgaaga aagttgagat cccaggtgtt gcgactaccg catcaccaag ttctgaagtc
    11101 ggtcgtttct ttgaaaccca gtcatcaaaa ggcggacgtt acactgtcac gcttcctgac
    11161 ggcactaaag tcgaagaact gaacaaagcg acagcagcga tgatggtccc gttcgacagc
    11221 atcaaattct ctggcaacta cggcaacatg accgaagtct cctatcaggt tgcgaaacgt
    11281 gccgcgaaga aaggtgctaa gtattaccac atcacccgcc agtggcagga acgtggtaat
    11341 aacctgaccg tcagcgcaga tctgtataaa taactattca gttactccta taaggcggct
    11401 tgatgccgcc tttcgcattt ttagtcacaa atttccccgc gaccattgca tgcagacaca
    11461 tctcttcgta aaatcccgcg cttcagtggc aaactccatt tttatgcgtt ttgccctaat
    11521 atttattctt cttatcacgt tttaatcact ggatatcgat ggaaaagaaa ctgggactga
    11581 gcgcactcac cgcgctggta ttaagctcaa tgctgggcgc gggtgttttc agtctgccgc
    11641 aaaatatggc ggcagttgcc agcccggcag cactgctcat cggctggggt attactggcg
    11701 ctggcatttt attgctggcc tttgccatgc tgatcctcac gcgcattcgt cccgaacttg
    11761 acggcggtat ctttacctat gcccgcgaag ggtttggcga gctaatcggc ttttgttccg
    11821 catggggata ctggctgtgc gcagtcatcg ccaacgtctc ctatctggtg atcgtttttt
    11881 ccgcgttaag cttttttacg gacacgccgg aattgcgcct gttcggcgat ggtaatacct
    11941 ggcaatcgat agtcggtgca tcggctttat tgtggattgt tcactttttg atcctgcgcg
    12001 gggtgcaaac cgctgccagc attaacctgg tggcgacact ggcaaaattg ttgccgctgg
    12061 gtctgtttgt tgtgctggcg atgatgatgt tcaaactgga taccttcaag ctcgacttca
    12121 ccggacttgc acttggcgta cccgtgtggg aacaggtgaa aaacaccatg ctgatcaccc
    12181 tgtgggtttt cattggtgtg gaaggtgccg tggttgtttc tgcgcgtgcg cgtaataaac
    12241 gtgatgttgg caaagcgaca ctgctggcgg ttctctccgc tctgggcgtt tacttgctgg
    12301 taacgctgct ttcgctgggc gtggtggcac gtcctgaact ggctgaaatt cgtaacccgt
    12361 caatggccgg tctgatggtg gaaatgatgg gaccatgggg cgaaatcatc atcgctgccg
    12421 gtttgatcgt ttccgtttgc ggcgcgtact taagctggac catcatggcg gcagaagttc
    12481 cgttcctggc tgccactcat aaagcattcc cacgcatttt tgcgcgccag aacgcacaag
    12541 ctgcgccatc cgcctcgctg tggttgacca acatctgcgt gcaaatttgt ctggtactca
    12601 tctggctgac cggttccgat tacaacacgt tgctgaccat cgcctcagaa atgattctgg
    12661 tgccctattt cctggtcggc gcgttcttgc tgaaaatcgc aacccgtcca ctgcataaag
    12721 cggtaggtgt cggtgcctgc atttatggct tatggttatt gtatgcctct ggtccaatgc
    12781 acttgctgtt atccgttgtt ctgtatgcgc cggggctgct ggttttcctg tatgcgcgca
    12841 aaacgcatac acatgataat gtactgaacc gtcaggaaat ggtgctgatt ggcatgctgt
    12901 taattgcctc agttccggcg acctggatgc tggtgggata acgttcatac catcgttgga
    12961 tagcaaagga gataacgatg ggtaaagccc agcccttgcc aatattaatt actggcggag
    13021 gtcgtcgcat cggcctcgcc ctcgcctggc atttcattaa tcaaaagcaa ccggtgattg
    13081 tcagctatcg gacgcactat ccagccattg atggactgat taaggcaggt gcgcagtgta
    13141 ttcaggctga tttttcgacc aacgacggtg tgatggcgtt tgccgatgaa gtactaaaaa
    13201 gcacccatgg tctgcgtgct attttgcata acgccagtgc gtggatggcg gaaaaaccag
    13261 gtacaccact ggccgacgta ctggcttgca tgatgcagat ccacgttaat accccatacc
    13321 tgctcaacca tgcgctggaa agattactgc gtgggcacgg acacgccgcc agcgatatca
    13381 ttcactttac cgattatgtg gtggagcgcg gtagcgacaa acatgttgcg tatgctgcaa
    13441 gcaaagcggc actggataat atgacccgct cgtttgcccg caagctggca ccggaagtga
    13501 aagtgaattc tattgcgcca tcgctgatcc tgtttaatga acatgatgat gccgaatatc
    13561 gacaacaggc gctgaataaa tcactgatga aaaccgcgcc tggcgagaaa gaagtgatcg
    13621 acctggtcga ttacttactt accagctgct ttgtcaccgg acgcagtttc ccacttgatg
    13681 gcggtcgtca tctgcgttaa tgcagcttta tccagcatat aatcaacacc catgcgctta
    13741 tcccccaaca cgcaacagaa ccaacaaaag cggcgggcaa gcgcatcatt ccggtgaaat
    13801 accacagcga caccaggtaa acaaaatagg gaataatcga ccacatacta aaaatgatgg
    13861 ttgcgcgtaa ggcttcaatg ccgcgttcgc tggcaacaat ataatgcgcg ataagcgcaa
    13921 aggtcgggaa aagtggaatc agcccggcga tataataatt tttcgttttt gctaaaacac
    13981 caatcaacag cactaccagc gcaccgagcg cggctttgat taccagcccc atctttttac
    14041 cttaacactt ccataacaag tcatcagtag aatacctgat gaaaacttgt ttagaaacga
    14101 ttgatagtaa gtaaaaacag cgcggtgtat tgtgacgttt ttatatctac cgtgaatgtt
    14161 atgaacacta tcgtatttgt ggaagatgat gcggaagtcg gttcactgat tgccgcgtac
    14221 ctggcaaaac atgatatgca ggttaccgta gaaccgcgcg gcgaccaggc cgaagaaacc
    14281 attttgcgag aaagtccgga tttggtgtta ctcgacatca tgctaccagg caaggacggc
    14341 atgaccattt gtcgtgattt acgcgcaaag tggtctggac cgattgttct tctaacctct
    14401 ctcgatagcg atatgaacca catcctggca ctggaaatgg gtgcctgcga ctatattctc
    14461 aaaacgacgc cccctgctgt tttgctagcg cgtttacgtt tgcatttgcg tcagaatgag
    14521 caagccacac tgaccaaagg tcttcaggaa acgtctctga ctccctacaa agccctgcat
    14581 ttcggcacgt tgaccatcga tcccatcaac cgcgtagtca ccctggctaa cactgaaatc
    14641 tcgctctcga cagctgattt cgaattattg tgggaattag ctacccatgc cgggcaaatc
    14701 atggaccgcg atgcattgct gaaaaattta cgcggcgtca gttatgacgg actggatcgt
    14761 agcgtggacg tggctatttc gcggttaaga aaaaaactgc tcgataacgc cgcagaacct
    14821 tatcgcatta aaactgtgcg taacaaaggc tatctttttg cgcctcatgc atgggaataa
    14881 gcgatgaaaa aactgtttat ccagttttac ctgttattgt ttgtctgctt ccttgtgatg
    14941 tctctgctgg ttgggttggt gtacaaattt accgccgaac gcgcgggcaa acagtcgctg
    15001 gatgatttga tgaacagttc gctgtatctg atgcgcagcg aattgcgtga gatcccccca
    15061 cacgactggg gtaagacgct gaaagagatg gatttaaatc tctctttcga tctgcgtgtc
    15121 gagccactga gtaaatacca tcttgatgat atttccatgc accgactgcg tggcggcgaa
    15181 attgtcgccc tggacgatca gtacacgttt ttgcagcgta tcccgcgcag ccactacgtg
    15241 ctggcagttg gtcctgttcc ttatctttat tatctccatc agatgcgatt gctggatatc
    15301 gccctgatcg cttttattgc tatttccctc gcctttccgg tgtttatctg gatgcgtccg
    15361 cactggcagg atatgttaaa actggaagca gcggcgcaac gatttggcga tgggcatctc
    15421 aatgagcgta tccactttga tgagggttcg agctttgaac gacttggtat cgcatttaac
    15481 cagatggcgg acaatatcaa cgccttgatt gccagcaaaa aacagcttat tgacggtatc
    15541 gctcacgaac tgcgaacacc gttagtgcgc ctgcgttatc gactggagat gagcgataac
    15601 ctgagcgccg ccgaatccca ggcgttgaat cgtgatatca gtcaacttga agctttaatt
    15661 gaagagctgc tgacttatgc ccgactcgat cgcccacaaa acgagcttca tcttagcgaa
    15721 ccagacctgc cgttgtggct gtcaacgcat ctggcagata ttcaggcagt aacgcccgat
    15781 aaaacggtac ggataaaaac gctcgtgcaa ggccattatg cggcgttgga tatgcgctta
    15841 atggagcgcg tgctggataa tttgctcaat aacgccctgc ggtactgcca ttcaacggtt
    15901 gaaaccagcc tgctactgtc ggggaataga gcgacattaa ttgttgagga tgatggccca
    15961 gggattgccc cagaaaaccg cgaacatatc tttgaacctt ttgttcgcct cgatcccagc
    16021 cgggatcgct caaccggcgg ctgcgggctg gggctggcaa ttgtccactc tatagcactg
    16081 gcaatgggcg gtacggttaa ttgtgacacc agcgaactgg gtggtgctcg cttctcgttt
    16141 agctggccgt tatggcataa catcccgcaa tttacctctg cctgacacta cgcgcacgat
    16201 ggtcaagtca ccacgactgt gctataaaat aagtatgttg taactaaagt ggttaatatt
    16261 atggcgcgtt acgatctcgt agaccgactc aacactacct ttcgccagat ggaacaagag
    16321 ctggctacat ttgccgctca tcttgagcaa cacaagctat tggttgcccg cgtgttctct
    16381 ttgccggagg taaaaaaaga ggatgagcat aatccgctta atcgtattga ggtaaaacaa
    16441 catctcggca acgacgcgca gtcgctggcg ttgcgtcatt tccgccattt atttattcaa
    16501 caacagtccg aaaatcgcag cagcaaggcc gctgtccgtc tgcctggcgt gttgtgttac
    16561 caggtcgata acctttcgca agcagcgttg gtcagtcata ttcagcacat caataaactc
    16621 aagaccacgt tcgagcatat cgtcacggtt gaatcagaac tccccaccgc ggcacgtttt
    16681 gaatgggtgc atcgtcattt gccggggctg atcaccctta atgcttaccg cacgctcacc
    16741 gttctgcacg accccgccac tttacgcttt ggttgggcta ataaacatat cattaagaat
    16801 ttgcatcgtg atgaagtcct ggcacagctg gaaaaaagcc tgaaatcacc acgcagtgtc
    16861 gcaccgtgga cgcgcgagga gtggcaaaga aaactggagc gagagtatca ggatatcgct
    16921 gccctgccac agaacgcgaa gttaaaaatc aaacgtccgg tgaaggtgca gccgattgcc
    16981 cgcgtctggt acaaaggaga tcaaaaacaa gtccaacacg cctgccctac accactgatt
    17041 gcactgatta atcgggataa tggcgcgggc gtgccggacg ttggtgagtt gttaaattac
    17101 gatgccgaca atgtgcagca ccgttataaa ccacaggcgc agccgcttcg tttgatcatt
    17161 ccacggctgc acctgtatgt tgcagattaa cgcccggctt tcatactgcc gaccatctgt
    17221 tctggccgta cccagctgtc aaactcggct tcgctaagat accccagcgc aagggccgca
    17281 gcttttaagg tcagcccttc tttatgcgct tttttggcga tctccgcggc tttgtcataa
    17341 ccaatgtggg tgttaagcgc agtcaccagc atcagcgatt cattgagtaa ttgattgatt
    17401 cgctcacgat tcagttcaat acccactgcg cagtgtttgt taaaactttc catgccatct
    17461 gccagcaagc gcaccgattg caggaaattg tggatcacca ttggacggaa gacgttcagt
    17521 tcaaagttac cggaagcgcc ccccatgttg atcgccacgt cgttccccat cacctgacag
    17581 cagagcatgg ttaatgcctc acactgtgtt ggattcactt tccccggcat gattgagctg
    17641 cccggctcat tttccgggat tgagatttca ccaattccgc agcgcgggcc agaggccagc
    17701 cagcggacat cattggcgat tttcatcagt gacacagcca accctttcaa cgcgccgtgc
    17761 gcctgaacca gggcatcaca ggtcgccagc gcttcaaatt tgttcggcgc ggtaacaaac
    17821 ggtgcacagg taatgactgc cagttcatct gctacgcgac gcgcatactc cggatgggta
    17881 tttagtccag tacccaccgc tgtaccgcca agagccagtt ccgctacgtg aggcaggctg
    17941 tattcgatat gtttgagatt atgctcgagc atcgctaccc agccggaaat ctcctgccct
    18001 agtgttagcg gcgtggcgtc ctgcaagtgg gttcgaccga ttttgacgat atcggcaaat
    18061 gcacgcgatt tttcactcag tgtctgtgtc agggttttaa gctgcggaat gagttgcttg
    18121 cgcagcgcca gcagcgccgc aacgtgcatc gccgtcggaa agacatcgtt ggaactttgg
    18181 cttttgttca cgtcgtcgtt agggtgaact ttacgttcca tcccgcgcac gccgccgagt
    18241 aattcactgg cccggttagc cagcacttcg ttcatgttca tgttactttg cgtgccggag
    18301 ccggtctgcc agatagccag cgggaattcg tcgtcatgct gtcctgccag tacttcatcc
    18361 gccgcctgcc gaatggcgct cgctttctct tcagacaaca agcctaaatc ttcattaact
    18421 tttgccgctg cgcgcttggt tagcgccagc gcatgaatca gtgaggtggg cattttctcc
    18481 gtcgaaatgc ggaaatgctc cagcgagcgt tgagtttgtg cgccccacag cttatctgcc
    18541 gggacatcaa tcgcccccat cgaatctttt tcgctgcgta ctgtattcat ggcctgctcc
    18601 tcacctgttt aattttttct ttctgttttg ctttcgttaa gcaactttta gctcacttat
    18661 tatttactat ttgataacaa atgtttggtc tttcgtgcca tgtaaaaaaa ccgccccgaa
    18721 gggcggctct gtttatttca cacagcgagt gcactgtgtg agttgtatct gctggaagaa
    18781 gtcatttcct ttatcatcca caaggataaa cgccgggaaa tcttccactt caattttcca
    18841 gatggcttcc attcccagtt ccggatattc aacacattcc aggctcttaa tacttccctg
    18901 cgccaataca gcggccggac caccgatact gccaaggtag aagccgccgt gttttttaca
    18961 ggcatccgtc acctgctggc tgcggttgcc tttcgccagc atgatcatac ttccgccctg
    19021 cgcttgcagt tgatcgacat aagaatccat ccgtccggcg gtcgttgggc caagagaacc
    19081 ggaggcataa ccttccggcg ttttggccgg acccgcgtag taaatcggat gatctttaat
    19141 gtactgcggc agcccttcac cgttatccat ccgctctttc agtttggcgt gagcaatatc
    19201 acgaccgacg ataatcgtgc cgttaagcga taagcgtgta gaaacgggat actgcgacaa
    19261 ctgtgcgagg atctctttca tcggacggtt aaggtcaacg cgcaccgctt cgccttctcc
    19321 cgctttacgc agctcttcag ggatatattt acctggatta tgttccagtt tttcgatcca
    19381 gatcccctga cggttgatct tcgctttgat attacggtca gcagagcagg aaacacccat
    19441 accaaccgga caggatgcgc cgtgacgtgg caggcgaatc acgcgtatgt cgtgagcgaa
    19501 gtatttacca ccaaactgcg cacccagacc gagattttgc gcttcgatca gcaattcttt
    19561 ttccagttcc acatcgcgga atgcctgacc gtgctcattc ccttccgttg gcagttcgtc
    19621 atagtattta gcggaagcca gtttcactgt tttaaggtta gtttctgcag aagttccacc
    19681 aattacgaac gcaatatgat acggaggaca ggccgccgta cccagcgtgc gcatcttctc
    19741 aaccaggtaa tttttcagtt tccccggcgt tagtaacgct ttggtttcct gatagagata
    19801 cgtcttgttt gccgaaccac cacctttggc gatacagagg aatttgtact cgtcgccatc
    19861 aacggcataa agatcgatct gcgctggcag attggtgccg gtattcacct ctttatacat
    19921 atccagcggc gcgttttgcg agtagcgcaa attatcttcg atataagtgt tatagacacc
    19981 gcgcgccagc gccgcttcat cacagccacc ggtccataca cgctgccctt ttttaccaac
    20041 aataatcgca gtgccggtat cctgacaggt tggcagaacg cctttcgccg cgatgtcgga
    20101 gttacgcagg aattgcagcg ccacatattt atcattttcg ctggcctccg ggtcacgcag
    20161 aatgtcggcc acctgttgtt ggtgagccgg acgcagcata aacgacgcat catgaaacgc
    20221 ctgacgcgcc aacagagtta acgcttcggg tgcgactttc aaaatctcct gcccttcaaa
    20281 ttcagatacg ctaacgtgtt cgctggttag caggtaatac tcagtatcat cttttttgag
    20341 tggaaaagga gcctgataat gaaagggttt gtttgacatt gttctctcac ttactgcctg
    20401 gtttggttat gctctgggcg ggtgttccgt tgccctgtta aaagcgagta acaatatcct
    20461 acacactttt ttaacaaaaa ctgagactag tacgactttt tgcggctcca ggttacttcc
    20521 cgtaggattc ttgctttaat agtgggatta atttccacat taaaacaggg attgatcatg
    20581 caaaaactca ttaactcagt gcaaaactat gcctggggca gcaaaacggc gttgactgaa
    20641 ctttatggta tggaaaatcc gtccagccag ccgatggccg agctgtggat gggcgcacat
    20701 ccgaaaagca gttcacgagt gcagaatgcc gccggagata tcgtttcact gcgtgatgtg
    20761 attgagagtg ataaatcgac tctgctcgga gaggccgttg ccaaacgctt tggcgaactg
    20821 cctttcctgt tcaaagtatt atgcgcagca cagccgctct ccattcaggt tcatccaaac
    20881 aaacgcaatt ctgaaatcgg ttttgccaaa gaaaatgccg caggtatccc gatggatgcc
    20941 gccgagcgta actataaaga tcctaaccac aagccggagc tggtttttgc gctgacgcct
    21001 ttccttgcga tgaacgcgtt tcgtgagttt tccgagattg tctccctact ccagccagtt
    21061 gcaggtgcac atccggcaat tgctcacttt ttacaacagc ctaatgccga acgtttaagc
    21121 gaactgttcg ccagcctgtt gaatatgcag ggtgaagaaa aatcccgcgc gctggcgatt
    21181 ttaaaatcgg cgctcgacag tcagcagggt gaaccgtggc aaacgattcg tttaatttct
    21241 gaattttacc cggaagacag cggcctgttc tccccgctgt tactgaatgt ggtgaaattg
    21301 aatcctggcg aagcgatgtt cctgttcgct gaaacaccgc acgcttacct gcaaggcgtg
    21361 gcgctggaag tgatggcgaa ctccgataac gtgctgcgtg cgggtctgac gcctaagtac
    21421 attgatattc cggaactggt tgccaatgtg aaattcgaag ccaaaccggc taaccagttg
    21481 ttgacccagc cggtgaaaca aggtgcagaa ctggacttcc cgattccagt ggatgatttt
    21541 gccttctcgc tgcatgacct tagtgataaa gaaaccacca ttagccagca gagtgccgcc
    21601 attttgttct gcgtcgaagg cgatgcaacg ttgtggaaag gttctcagca gttacagctt
    21661 aaaccgggtg aatcagcgtt tattgccgcc aacgaatcac cggtgactgt caaaggccac
    21721 ggccgtttag cgcgtgttta caacaagctg taagagctta ctgaaaaaat taacatctct
    21781 tgctaagctt attaaaggct tataacacct tcaggcggcc agtccgcctg atttcatttt
    21841 atggataatc attatgaata aatcgctggt agcggcaggc gtcattgttg cgctaggcgt
    21901 agtctggaca ggcggcgcat ggtatacagg caagaagatt gaaacccatc tcgaagacat
    21961 ggtcgcgcag gcgaacgcgc aactcaaact gacagctcct gaatccaacc tggaagtgag
    22021 ttatcaaaac tatcatcgcg gcgtattcag cagccagttg caactgttgg tgaaacccat
    22081 tgccgggaaa gaaaatccgt ggattaaaag cggtcagagc gtcatcttca acgaatcggt
    22141 tgatcatggt cccttcccgc ttgcccagct taaaaaactg aacctgatcc cgtcgatggc
    22201 atcaattcaa accacgctgg ttaataacga agtaagcaaa ccactgtttg atatggcaaa
    22261 aggtgaaacg ccttttgaga ttaactcgcg cattggttac agcggtgatt ccagttccga
    22321 tatttcgctc aagccactga attacgagca aaaggatgaa aaagtcgcct ttagcggcgg
    22381 cgagttccag ttaaatgctg acagagacgg caaagccatc tccctttccg gggaggcgca
    22441 aagtggtcgg atagacgcag ttaacgaata caaccagaaa gtgcagttga cctttaataa
    22501 tctgaaaacc gacggttcca gcacgctggc aagttttggt gagcgcgtag gaaaccaaaa
    22561 actgtcactg gaaaaaatga ccatttcagt ggaaggcaaa gaactggcac tgctggaagg
    22621 catggagatc agcggtaaat cggatctggt caatgacggt aaaacgatca atagccaact
    22681 ggattactcg ctaaacagcc tgaaggtaca gaatcaggat ctgggcagcg gcaagctgac
    22741 tttaaaagtc ggccagattg atggtgaagc ctggcatcag tttagccagc aatataacgc
    22801 gcaaactcag gcgctgctgg cacagccaga aattgccaac aatcccgaac tttatcagga
    22861 gaaagtgacg gaagccttct ttagcgccct gccgctgatg ttgaaaggcg atccggtgat
    22921 tactatcgcg ccgctaagct ggaaaaacag tcagggtgaa agtgcgctga atctgtcgct
    22981 gttcctgaaa gatccggcaa cgactaaaga agcgccgcaa acgctggcgc aggaagtaga
    23041 tcgttcggtt aaatctctgg atgcgaaact gaccattccg gtggatatgg caactgagtt
    23101 tatgactcag gtagcgaagc tggaaggtta tcaggaagat caagcgaaaa aactggcgaa
    23161 acagcaagtt gaaggtgcat cagcaatggg gcagatgttc cgtctgacca ccttgcagga
    23221 caataccatc accaccagcc tgcaatatac taacggtcag ataacgttaa acgggcagaa
    23281 aatgccactg gaagatttcg ttggtatgtt tgcaatgccg gcattaaatg ttccggtcgt
    23341 acccgctatt ccgcagcagt aattcacaga caaaacgccg gagatttttc tctccggcgt
    23401 tattttttac ttcagcataa agtcatactg aatactcatt ttcacatccc agtaattcag
    23461 gcgatcgccc agcgtactgc catcgcgctt ttcccgccct ttatcggcag caagaaagaa
    23521 attcatccct tgtaacggcc cttcgctgac ccgatagttc ataccaccta agatacccgt
    23581 gccttcaatc cgcacatctc gtttactgga ttcatagccg cgagtcacaa tcggtgcaaa
    23641 tgtcagcgta aattgcggcg ttaaacgata gttgacgccc aattgccagg actgcattcc
    23701 ttcacggtta cgatcaagac ttaatgaaaa gggatagcca atgtcggtat ccaccacggc
    23761 accattcatt gcatggcgca aataaccacc actgccaaat acgcttcccc acggcgcatt
    23821 ccaggtcaat tgtccactaa ccaacgccgt ttcattgggc tggctggtat tacgacttaa
    23881 accttcgagc tgcgcgtaaa agccgagcaa ctcccctttc aacgtataat tcgggtcgag
    23941 cggtatgctt ttctcgagaa tactttgtat ctgattggaa acatctttct gtaccgatgc
    24001 ccccagagaa tagcgactgg tatcatcatg ccaactggcg gctaaaaaag aacgtgcgcg
    24061 atgtttatcg tattttcccg tctggctgtt atacacccct tcatcacgaa taccggttgt
    24121 tgaatcagtc cataaaaata ctcgtgaacg ccacattccc cacagatcaa tatttggtgt
    24181 taaagcccaa taacctaatg ctccttcaga aactgtcggt aatgtacgtt gcgaactgcc
    24241 agccattaac ggaatacgat aaggtaagct accataacta taatccgtcc cgaagcgacc
    24301 tagtttaagc aggctatttt cactgggttt tatttttact gcccccaggg caaagccaaa
    24361 acttttatca ccatcaagat accaccgggt actcatatca gcacgagcac ctaatttata
    24421 aacataatat gccccgcctt caacgccaat aatattggaa taataaccac tattgaaatc
    24481 gagcaatcct ccctgtaccc atgcgtaaat atctcttccc gcgccagcac tgggcttatc
    24541 cgccctgcgc aattcgtttt tcagtctgat gcgtagtccc gcttcatcgt cagccagttg
    24601 cgccgcataa gcagaggtaa ggccagaggc agccgtcagg caaataacgg ccatggccac
    24661 tatttttctc atagttgcac ctttgatgtt ctgattattt ttattgaata ttaattagtg
    24721 atatcgctga ttaattgctg ctgcactttt ttacgattat caatttcaac cacgatttct
    24781 ttgaattttt tatccgtgag cggataaaac cagataataa cgaatgccag tagcataaat
    24841 ccgcaaggta ctaaggcaat tgatgtgcgg atgcccataa taacttccgg cgtttgcacc
    24901 tgattggcga tatatccgct taatcccaaa ataaaggcag gaattgaacc tccgattgcc
    24961 tgaccgcatt tacgggtaaa tgagaatagt gaataggtga gcccttcaat tcgcacgccg
    25021 gtcaggtatt caccgtattc tacggtatca gcttccagtg cccacatcac agtcatagta
    25081 acgccctgac caattgaagc gatggccaac gcaaccaacg ccaccggcag tgaccagacg
    25141 gaaacccaga agaatagcag ataaccgcag gttcccagca aagcgccaat caggaaggta
    25201 ttctttttac cgatcctcgc gaccatcccc ggcaccagcg gtgccgatgc cacagtacca
    25261 accaggtttt gcaccagtac cagcacagtg aacagcccgg tatcatttaa cacatagcgc
    25321 acgtagaaca acgacgaggc gctgaccgcg aaggtcgaaa tcagcacaca cagcgcaccg
    25381 atgcacaaca taaacagcgg gcgattccgt ttcagggttt gcagactgat cttcaatgac
    25441 ggctgcgcca cgatacgtac cacattctca cgcgtcgatt tgaagcagat gaagtaaagc
    25501 accattccgg caatcgccag cacaatcgtc cagaaatggt ataccgacac catctcttcc
    25561 gggctggagt tcttaatgct cggtcctatc agaaatgcca ggcagacaaa ggttaatgaa
    25621 gcggcaatcc cgcgagccgc gcccagacgg gcgcgggatt gtggttgttg ggtcatcgcg
    25681 gtagcaagtg aaccataagg aatattcacc aggctgtagc aaagcccgag gcccatgtag
    25741 gtcaaatatg catacaccac tttgctacca tggctccagt cggtcggcac ccagaatacc
    25801 agcacgctga agatcattaa cggcgcagta ccgaagagta aaaacgggcg gaattttccc
    25861 cagcgggtat tcacactgtc caccactcgt ccggcaaaga cgtcggcgaa ggcatcgaat
    25921 acccgcacca gtaacagcat ggtgcccgcc gcagcggcac cgacgccagc gacgtcggtg
    25981 tagtaactca ataggaagag cgcccccatt gcgaaggcga agttattggc gacgtcaccg
    26041 aggctgtagc cgacgatggt gcgccaggag agttgttgat tcattgtttg cctccctgct
    26101 gcggtttttc accgaagttc atgccagtcc agcgtttttg cagcagaaaa gccgccgact
    26161 tcggtttgcg gtcgcgagtg aagatccctt tcttgttacc gccaacgcgc aatatgcctt
    26221 gcgaggtcgc aaaatcggcg aaattccata cctgttcacc gacgacggcg ctgacgcgat
    26281 caaagacgcg gtgatacata tccagccatg cacactgata ctcttcactc cacatgtcgg
    26341 tgtacattga gtgcagcccg gctaacgtat ccacgccgta ttcggtgatg ataatcggct
    26401 gatgcagttt ctcctgccag gccagaagtt ctttttccag taccttctct gccgtttcca
    26461 aatcgccgct ttggacatac catccgtaat aacggttcag gcacagcaca tcaaagagat
    26521 cgctgatggt atcggtgtga gcgtcgcaga acattacatt gacgcaggtg atcggacgcg
    26581 tcgggtcgag tttacgcgtt gcttccgcca gtggcgcgaa atattcccgt gcaccttgcg
    26641 gacgggtatc cggttcgttg gcaatactcc acatcaccac gcttgggtgg tttttgtcac
    26701 gcgctatcag ctctttaatc gcctgtaagt gcgcttgctg agtttccccg ttgactgcct
    26761 cttcgctgta cagttctttc ggcttgttgc ccgcttcgaa accaatgcct aaagagaggt
    26821 taaagccgac agcagcagtt tcatcaatca ccacgatgcc atgttcatct gcccagtcga
    26881 gcatctcttc agcgtaaggg taatgcgagg tacggtagga gttggcccca atccagtcca
    26941 ttaatgcgtg gtcgtgcacc atcagcacgt tatcgaatcc tttgccacgc aagtccgcat
    27001 cttcatgacg accaaagcca gtaaagtaga acggtttgtg gttaatcagg aactgttcgc
    27061 ccttcactgc cactgaccgg atgccgacgc gaagcgggta gatatcacac tctgtctggc
    27121 ttttggctgt gacgcacagt tcatagagat aaccttcacc cggttgccag aggtgcggat
    27181 tcaccacttg caaagtcccg ctagtgcctt gtccagttgc aaccacctgt tgctccgcat
    27241 cacgcagttc aacgctgaca tcaccattgg ccaccacctg ccagtcaaca gacgcgtggt
    27301 tacagtcttg cgcgacatgc gtcaccacgg tgatatcgtc cacccaggtg ttcggcgtgg
    27361 tgtagagcat tacgctgcga tggatcccgg catagttaaa gaaatcatgg aagtaagact
    27421 gctttttctt gccgttttcg tcggtaatca ccattcccgg cgggatagtc tgccagttca
    27481 gttcgttgtt cacacaaacg gtgatacgta cacttttccc ggcaataaca tacggcgtga
    27541 catcggcttc aaatggcgta tagccgccct gatgctccat cacttcctga ttattgaccc
    27601 acactttgcc gtaatgagtg accgcatcga aacgcagcac gatacgctgg cctgcccaac
    27661 ctttcggtat aaagacttcg cgctgatacc agacgttgcc cgcataatta cgaatatctg
    27721 catcggcgaa ctgatcgtta aaactgcctg gcacagcaat tgcccggctt tcttgtaacg
    27781 cgctttccca ccaacgctga tcaattccac agttttcgcg atccagactg aatgcccaca
    27841 ggccgtcgag ttttttgatt tcacgggttg gggtttctac aggacgtaac ataagggact
    27901 cctcattaag ataaaaatac tggtcaacct ttaatctgat tagttaacca attctgtgtt
    27961 gcacacagtt atctgtgaga aacccatgga agagaaatgg aaataaaaag aactgacacc
    28021 gcgatcaaaa aaccaaaaac tgcaataccc acagcgtgat attgatcgca ttaatcttta
    28081 aaacggcgtt gacagaagcc ataaaataag caaccgaacg ttcgttcgag catattggga
    28141 acgataaact ctcatgacat atggctacgg tgaatatttt tgggggggaa ttctccgatc
    28201 ccggctagat tgtattttgt acagggaaat taaagtatga aacgcgggca cagttatcac
    28261 cagcccgcga aatgagagga tcaggatgcg gttaagatac cgccaatcat cgcgcgtaat
    28321 ccctgagcaa acgacgcttc acgcgccaga tcttccgctt cgatatcggc cagtacaccg
    28381 taggtcagat ccagcagtaa acgtgaagtc attgacgtgt tgatgtctgg gcttatctcg
    28441 cctttttgct gggcatcacg catccgctcg gcaacaaatt cagtgatcgt catatgctta
    28501 tttttaagca tggcggcgac ctgtgggtta cgcatccctt ccgccataat ttcgaccacc
    28561 agcgcccgtt gcccaaaggc ttcatgggtg agagagacaa tagactcgac catatagtca
    28621 acaaaatgaa tcccttcaat cggttcccgg aaacgggcca gcgccctctc ctggtcctgt
    28681 aagataatcg cctgaatcaa ggcttctttg gagatgaaat ggtgatagag cgtcccggga
    28741 ctaatggcgc aggatttaca gatggctttc atcgaggcac tgtgaaatcc attttctgaa
    28801 aaaatctctc tggcagcatt gaggatccgg gtccgtgtcg gttgtgcttc agtctgcatg
    28861 ttatccatca taaaaccatt atagttagcg tgaataacta gtttaccgcg ttgtcaggtg
    28921 agcgcctatc attgctgttg aatagtgcga aggcacactc tattcgtata cttttcactc
    28981 tgaaattggt taaccaatag ccgtgatttg gctgccagtt atttagcaga gacgttgatg
    29041 agtttatccc ggtaaacaac acgcacggtt gtttaccggt ccgttagtgt attaattgag
    29101 ctcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tcgcagcagg
    29161 cgagcaaagg aacagcgctg cgttagcaat atcttgcggt tggcccagac gtctgatcgg
    29221 cgtgtgctgt aacatttttt gttcaatttc tggtgtaata acggatttca gggcatcggt
    29281 taatattgcc cccggcgcaa tgccatttac ccgaatattt ttttcaccca ggtcaaacgc
    29341 catatttctg accagatgac tggctgcagc tttagatgat gcataggaag tcatgtttat
    29401 atttttattt tctgccgcca tagaagtgat ggtcagaata acgccaccgc catttttttc
    29461 catttctggc gcaacaagtt gtgacagatg gaaaaaagaa aacacattca gttcataagc
    29521 acggcgaaaa tccgccattg gcatatcaaa cggtttaggt ccaccgccac cggcgttgtt
    29581 aaccagaata tcaactttac ccagcttact gatagcaaag tctgccagtg cagagagttc
    29641 ctgttcggaa gtaatatcac aacggcaggc aaatgcctga ccacccagtt gttgaatttc
    29701 gtctacaaca tggttagctg cgtcggcgtt aatatcactg accaccacag atgcgccagc
    29761 tgtcgcgaat gtaatggcga tttctttacc aatacctgca cccgcacctg tgatgatggc
    29821 gcattttccg tcgagtctca ggttgtcaga attaaacaca taaacctcct gtgaacttca
    29881 gtaaaaataa aaggcaccgc ctgtgtgcca cattttttat cattaacctt gaggaaagga
    29941 tactaccagg tatagcagat aaagagagga ttatttcgcc gcaatgagcc gggcgggaat
    30001 aataagattg cgtgaatgcg tctcttcatg ggtgattttt tgcatcatgc gatccgcaag
    30061 tgtgatacca agttcccgcg ctggcgtgct ggcccaggta acaggtatat catcaagtgt
    30121 ggttggtgtc gcatcggtaa atgccgccag cgaaacctgt tgctcaaagt aacgatcgac
    30181 tccgctttcc ccgctttgcc gccctgcttt gagcaaacca aaccatgccc ccatcgcaat
    30241 agtttcgtta tagcaaacca cggcactgat ggtcgggtta tgacgtaaaa gcgccgtgat
    30301 agcttccgcg gcttgcttct ggctggaagt gcactccaac acccaatcgc tatgaaacgg
    30361 caggccaaat tttagtagag ttgcacaata gccccccacc cgttctgcac gggttaatga
    30421 ggaactttgc cctcccagcc aggcgatccg ctgatgccca ttgcgaatga gatgctccgt
    30481 caacaactgt gcagcctgca tgttgtccgg gcgaaccgta tcaacatcat caagataact
    30541 ggcacgggaa gcgaaaatca ccgggatagc tttttcttct gccatccgtc gcaggtcatc
    30601 gctgcttcct gcagccccgg caattaccac accatccaca ccctgattca gtaacagtga
    30661 aaaccgctgt gccagctgct cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc
    30721 ctgcgcttcc agagcttccg tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa
    30781 atcacggacg atcaaaccaa tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg
    30841 attgcgcaca aatcccagct cttcaatggc ggcgttaacg cgttctcctg tggcggtaga
    30901 gattcgccct ttgccactaa gcaccagcga aacggtactt accgacacgc ccgcagccag
    30961 cgcaacatca tgaatggtta tttttttggc ggtagccatg cagatgacct actccctgat
    31021 tatgtgacag ataaaacgtt ttacctttta tttatcttat actcgctatt atcgttgcgt
    31081 aatgtgattt atgcctcact aaaatttgat aaaacgtttt atcttctcgc gcaatttact
    31141 gaatccagat tgttctctac gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc
    31201 acgctgtggg agttcttcca gcagttaggc aaaaccttca tgttacccgt ggcattattg
    31261 tcgttctgcg gcattatgct cggcattggt agttctctta gtagccacga tgtcatcacc
    31321 ctgatcccgg tcctgggcaa ccccgtgttg caggctatct ttacctggat gagtaagatt
    31381 ggctcgtttg cttttagttt cctgccagtc atgttctgta tcgccatccc gctgggtctg
    31441 gcacgcgaaa acaaaggcgt agcggcattc gctggcttcg tcggttatgc ggtaatgaac
    31501 ctcgcggtaa acttctggtt gaccaataaa ggcattctgc caaccacgga cgccgcggtt
    31561 ctgaaagcca ataacatcca gagcattctt gggatccagt cgatcgacac cggtatcctc
    31621 ggtgcggtga tcgccggtat tatcgtctgg atgctgcatg agcgattcca taatatccgc
    31681 ctgccggatg cgcttgcatt cttcggcggt acgcgcttcg taccaatcat ctcctcgctg
    31741 gtgatgggcc ttgtcggcct ggtgattcca ttagtctggc cgattttcgc gatgggtatt
    31801 agcggcttgg gccatatgat aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc
    31861 ggtgaacgtc tgctgttgcc gtttggtctg catcacattc tggtggcatt aattcgcttt
    31921 accgacgcag gcggcacgca ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc
    31981 ttccaggcgc aattgagttg cccgaccact cacggttttt ctgaaagcgc cacgcgtttc
    32041 ctttcgcaag gtaaaatgcc tgcgtttctc ggcggtctgc caggtgcagc gttagctatg
    32101 tatcactgcg cgcgcccgga aaatcgccat aaaattaaag gtctgctgat ttctggcctg
    32161 atcgcctgcg tcgttggcgg cactaccgaa ccgctggaat tcctgttcct gttcgtagcg
    32221 ccagttctgt atgtcatcca cgcgctgtta accggcctcg gcttcaccgt catgtctgtg
    32281 ctcggcgtca ccatcggtaa taccgacggc aatatcatcg acttcgtggt gttcggtatt
    32341 ttgcatggtc tgtcaaccaa gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc
    32401 gtttactacg tcatcttccg tttcgctatc acccgcttca atctgaaaac cccggggcgc
    32461 gatagcgaag ttgccagctc aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt
    32521 tacaacgttc ctgcaatcct cgaagcatta ggcggtgccg acaatattgt cagcctcgat
    32581 aactgcatta cccgtctgcg tttgtctgtg aaagatatgt cgcttgttaa tgtgctggca
    32641 ctgaaggaca atcgggcaat tggcgtagta caacttaatc aacataacct gcaggttgtt
    32701 atcgggccac aagttcagtc agtaaaagat gaaatggccg gtctgatgca tactgtccag
    32761 gcataaggat aagatatgtt cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt
    32821 acacagtggg attatgtcgc tgaccgtttc ggcactgctg acctgttacc gttcacgatt
    32881 tcagacatgg attttgccac tgccccctgc attatcgagg cgctgaatca gcgcctgatg
    32941 cacggcgtat ttggctacag ccgctggaaa aacgatgagt ttctcgcggc tattgcccac
    33001 tggttttcca cccagcatta caccgccatc gattctcaga cggtggtgta tggcccttct
    33061 gtcatctata tggtttcaga actgattcgt cagtggtctg aaacaggtga aggcgtggtg
    33121 atccacacac ccgcctatga cgcattttac aaggccattg aaggtaacca gcgcacagta
    33181 atgcccgttg ctttagagaa gcaggctgat ggttggtttt gcgatatggg caagttggaa
    33241 gccgtgttgg cgaaaccaga atgtaaaatt atgctcctgt gtagcccaca gaatcctacc
    33301 gggaaagtgt ggacgtgcga tgagctggag atcatggctg acctatgcga gcgtcatggt
    33361 gtgcgggtta tttccgatga aatccatatg gatatggtct ggggcgagca gccgcatatt
    33421 ccctggagta atgtggcgcg gggagactgg gcgttgctga cgtcgggctc gaaaagtttc
    33481 aatattcccg cgctgaccgg tgcttacggg attatagaaa atagcagcag ccgtgatgcc
    33541 tatttatcgg cactgaaagg acgtgatggg ctttcttccc cttcggtact ggcgttaact
    33601 gcccatatcg ccgcctatca gcaaggtgca ccgtggctgg atgccttacg cgtctatctg
    33661 aaagataacc tgacgtatat ctcagataaa atgaacgccg cgtttcctga actcaactgg
    33721 cagatcccgc aatccactta tctggcctgg cttgatctac gtctgttgaa tattgacgac
    33781 aacgcgttgc aaaaagcgct tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc
    33841 tatggtgaag aaggtcgtgg ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg
    33901 gaaaaaggtg tggctggatt aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc
    33961 aacgtaaaaa atcgttgcgc aatcgtggat ttttaccctg ctttgttttt ataatggtgc
    34021 gcacttttat attcagaaaa agagtgcgac catgattgat accaccctgc cattaactga
    34081 tatccatcgc caccttgatg gcaacattcg tccccagacc attcttgaac ttggccgcca
    34141 gtataatatc tcgcttcctg cacaatccct ggaaacactg attccccacg ttcaggtcat
    34201 tgccaacgaa cccgatctgg tgagctttct gaccaaactt gactggggcg ttaaagttct
    34261 cgcctctctt gatgcctgtc gccgcgtggc atttgaaaac attgaagatg cagcccgtca
    34321 cggcctgcac tatgtcgagc tgcgtttttc accaggctac atggcaatgg cacatcagct
    34381 gcctgtagcg ggtgttgtcg aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt
    34441 tggtgtgcag gcgaagctta tcggcattat gagccggacc ttcggcgaag ccgcctgtca
    34501 gcaagagctg gaggcctttt tagcccaccg tgaccagatt accgcacttg atttagccgg
    34561 tgatgaactt ggtttcccgg gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc
    34621 gggctggcat attaccgtcc atgcaggcga agctgccggg ccggaaagca tctggcaggc
    34681 gattcgtgaa ctgggtgcgg agcgtattgg acatggcgta aaagccattg aagatcgggc
    34741 gctgatggat tttctcgccg agcaacaaat tggtattgaa tcctgtctga cctccaatat
    34801 tcagaccagc accgtagcag agctggctgc acatccgctg aaaacgttcc ttgagcatgg
    34861 cattcgtgcc agcattaaca ctgacgatcc cggcgtacag ggagtggata tcattcacga
    34921 atataccgtt gccgcgccag ctgctgggtt atcccgcgag caaatccgcc aggcacagat
    34981 taatggtctg gaaatggctt tcctcagcgc tgaggaaaaa cgcgcactgc gagaaaaagt
    35041 cgccgcgaag taacaaaatg gatggtgcaa attgcaccat ccatttttca tgcaaggcac
    35101 aaagtcgcgc gatgtttggc ggattcgatg cccagctcaa tcaactccat tacctggatt
    35161 gcctggcttg ccggaaccgg attttcacca tcgccattta acgcatcacg aatagccgca
    35221 tagtaagccg gataattccc aggtaccgtc aacagcgttt cttcgacacg ttcctcacct
    35281 tccacgcggg taagtacgcc atcacgcata tcgtagcccc agtcttcctg cggcagacgc
    35341 tcgccatttt tcagacgttc ttcctgtgga tcgaggccat atttcacata actgcctcgg
    35401 gatccatgca cgatataccg tgctgactca gcggctgcca gcatggtacc gtgtaaaatg
    35461 actcgccgct gtggatagga caagatggcg tggaaataat cggtcgactg cgctccgggc
    35521 cgtaactgtg ccaaatctac cgtcatgctg accggtaaac caaaaagcgt aatggcctga
    35581 tcaagaagat gtggtgctaa atcgtaccag ataccgctgc ctgggccgcc ctgttcacgc
    35641 caacgatcgc gcacctgcgg acggaagcgg tcaaaatgag actcaaagta agcaacttca
    35701 cccagcacgc cttccgcgag taaacctttt agcgtcaaga aatcgctatc ccagcgacgg
    35761 ttatggaata cagacagcac acgccccagg ctttttgcca gcgcatccag ctctcgcgct
    35821 tgtgacagtg tcacggtaaa gggtttatca acgaccacat gtttacccgc ctcaagcgcc
    35881 gctttggcta acgggaaatg ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg
    35941 ggatcgttaa acagatgctt cggctcagag acaaccgtaa ccgttggcca gtcggctttt
    36001 acttttgttt catcactgct ggagattacc gccagttcca gcccgggcgt gcccgcaatc
    36061 aggggcgcat ggaaggtttt gctcgcataa ccatacccaa tcaacccaac acggatgttg
    36121 tcgctcatgt cattgcctct cattgaagta tgatggctat ttgacactat cctttaccca
    36181 cgctcaacag tttaataacc tgccagcaat aagggatgtt gtttaactta agtcaaaaaa
    36241 atagcgaatt ttccaacgac aaaagctaaa tatcgcaaaa acctcagtaa aaatcttgct
    36301 ggagctatta ttgctaagta acatttaccc cctgaagtta atggatcaat caagagagat
    36361 gtgggctgta atgaatcgtc ttattgaatt aacaggttgg atcgttcttg tcgtttcagt
    36421 cattcttctt ggcgtggcga gtcacattga caactatcag ccacctgaac agagtgcttc
    36481 ggtacaacac aagtaagctc tgcacttgtg gagcgacatg ctgcccgtcc gggtgcatgt
    36541 tttcacttgt cggatattaa accaggaatt tattatcttg ttcgatgttg ttggtgattg
    36601 tcagggatag taaagcgcgt tactcttttc agagcagttt ttcatccctg aatatttgcc
    36661 atcagcgcat tcttattatt gtaataacac aagcgttcac ttttggtgaa attttatcat
    36721 cactaataat acctacccct ttggtattaa ttattttatg gaccttttat gactgttcag
    36781 gactacttat taaaatttcg caaaatcagt tcactcgaaa gtctggaaaa actctacgac
    36841 catcttaatt acaccctgac ggacgatcag gaactgatca atatgtatcg tgctgccgat
    36901 caccgtcgcg cagagctggt ttctggcggg cgtttgtttg acctcggcca ggtaccgaag
    36961 tccgtctggc actatgtcca ataaagaaag tagcgatcgg ttataaaagc cttataataa
    37021 cgcccctaaa attagatttg cctgtttgcg tcgctattct ggagaaccta tgaccaccac
    37081 aacgccacaa cgtattggag gctggttgct tggcccgttg gcctggcttt tagtcgctct
    37141 gttgagtacg acgctggcgt tgttgctgta taccgctgcg ttatcttctc cgcaaacatt
    37201 tcaaacgctc ggcggacaag cactgacgac acaaatctta tggggcgttt cttttattac
    37261 cgctatcgcc atgtggtatt acacactgtg gttgaccatc gctttcttta aacgtcggcg
    37321 ttgtgttcct aaacactata ttatctggct gcttatttcc gtattgctgg cagtaaaagc
    37381 attcgctttt tcacccgtgg aagatggcat agccgttcgc cagttgctgt ttactttgtt
    37441 ggcaaccgca ttgatcgtcc cctatttcaa acgctcgtcg cgggtaaaag cgacgtttgt
    37501 gaatccgtaa taaccttaca gttaacctgt tgtcgcctgc tctggattaa cggataatag
    37561 gcggcttttt tatttcaggc cgaaaaatga ctgactacct gttactgttt gtcggaactg
    37621 tactggtcaa taactttgta ctggtcaagt ttctcggtct ctgtccgttt atgggggttt
    37681 ccaaaaaact ggaaaccgcg atgggcatgg ggctggcaac aacgtttgtg atgacgctgg
    37741 cgtctatttg cgcctggctt atcgatacgt ggattttgat cccacttaat ctgatttacc
    37801 tgcgcaccct ggcatttatt ctggtgattg ctgtggtcgt gcagttcacc gagatggtgg
    37861 tgcgcaaaac cagcccggtg ctttaccgtc tgctggggat ttttttgccg cttatcacca
    37921 ccaactgtgc agtgctcggc gtggcgttgc tgaatatcaa tctcgggcac aatttcttgc
    37981 agtcggcgct gtacggtttt tccgccgcta tcggtttctc gctggtgatg gtgctcttcg
    38041 ccgccatccg cgaacgcctt gctgtggctg atgtcccggc accttttcgc ggtcatgcca
    38101 ttgcgttaat taccgcaggt cttatgtctc tggcctttat gggctttagt ggtttggtga
    38161 agttgtaatg aatgctatct ggattgccgt tgccgccgtg agcctgctgg gcctggcgtt
    38221 tggcgccatt ctgggttatg cctcccgccg ttttgcggtg gaagacgatc cggtcgttga
    38281 gaaaattgac gaaatcttac cgcagagcca gtgtggtcag tgcggttatc ccggctgtcg
    38341 cccctacgcg gaagccatca gctgtaacgg tgaaaaaatc aaccgttgcg ccccaggtgg
    38401 cgaagctgtg atgctaaaaa ttgccgagtt gcttaatgtc gagccgcagc cgctggatgg
    38461 cgaagcgcaa gagataacgc ctgcgcggat ggtggcggtt attgatgaaa ataactgtat
    38521 tggctgcact aaatgtattc aggcgtgtcc ggtagacgcc atcgttggcg ctacccgtgc
    38581 catgcatacg gtaatgagtg atctctgtac gggctgcaat ttatgtgttg atccgtgccc
    38641 gacgcactgc atctcgttgc aaccggtcgc agaaacacct gactcctgga aatgggatct
    38701 gaacaccatt cccgtgcgta tcattcccgt ggaacaccat gcttaagtta ttctctgcat
    38761 tcagaaaaaa taaaatctgg gatttcaacg gcggcatcca tccaccggag atgaaaaccc
    38821 agtccaacgg tacacccctg cgccaggtac cactggcgca gcgttttgtt attccactga
    38881 aacagcatat tggcgctgaa ggtgagttgt gcgttagcgt cggcgataaa gtattgcgcg
    38941 gccagccgct tacccgtggt cgcggcaaaa tgctgcctgt tcacgcgccc acctcgggta
    39001 ccgttacggc tattgcgccc cactctacgg ctcatccttc agctttagct gaattaagcg
    39061 tgattattga tgccgatggt gaagactgct ggatcccgcg cgacggctgg gccgattatc
    39121 gcactcgcag tcgcgaagag ttaatcgagc gcatacatca gtttggtgtt gccgggctgg
    39181 gcggtgcagg attcccgaca ggcgttaaat tgcagggtgg cggagataag attgaaacgt
    39241 tgattatcaa cgcggctgag tgcgagccgt acattaccgc cgatgaccgt ttgatgcagg
    39301 attgcgcggc tcaggtcgta gagggtattc gcattcttgc gcatattctg cagccacgcg
    39361 aaattcttat cggcattgaa gataacaaac cgcaggcgat ttccatgctg cgcgcggtgc
    39421 tggcggactc taacgatatt tctctgcggg tgattccaac caaatatcct tctggcggtg
    39481 ctaaacaatt aacctacatt ctgaccggga agcaggttcc acatggcggg cgttcatccg
    39541 atatcggcgt attaatgcaa aacgtcggca ctgcttatgc agtgaaacgt gccgttattg
    39601 atggcgagcc gattaccgag cgtgttgtaa ccctgactgg cgaagcaatc gctcgcccgg
    39661 gcaacgtctg ggcacggctg gggacgccag tgcgtcattt attgaatgat gccggattct
    39721 gcccctctgc cgatcaaatg gtgattatgg gtggcccgct aatgggcttt accttgccat
    39781 ggctggatgt cccggtcgta aagattacca actgtctgtt ggctccctct gccaatgaac
    39841 ttggcgaacc acaggaagaa caaagctgca tccggtgtag cgcctgtgct gacgcctgcc
    39901 ctgcggatct tttgccgcaa cagttgtact ggttcagcaa aggtcagcaa cacgataaag
    39961 ctaccacgca taacattgct gattgcattg aatgtggggc ttgcgcgtgg gtttgcccga
    40021 gcaatattcc cctggtgcaa tatttccgtc aggaaaaagc tgaaattgcg gctattcgtc
    40081 aggaagaaaa gcgcgccgca gaagccaaag cgcgtttcga agcgcgccag gctcgtctgg
    40141 agcgcgaaaa agcggctcgc cttgaacgac ataagagcgc agccgttcaa cctgcagcca
    40201 aagataaaga tgcgattgct gccgctctgg cgcgggtgaa agagaaacag gcccaggcta
    40261 cacagcctat tgtgattaaa gcgggcgaac gcccggataa cagtgcaatt attgcagcac
    40321 gggaagcccg taaagcgcaa gccagagcga aacaggcaga actgcagcaa actaacgacg
    40381 cagcaaccgt tgctgatcca cgtaaaactg ccgttgaagc agctatcgcc cgcgccaaag
    40441 cgcgcaagct ggaacagcaa caggctaatg cggaaccaga agaacaggtc
//
LOCUS       DAZFJF010000030        39929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFJF010000030 DAZFJF010000000
VERSION     DAZFJF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.030"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            117..1447
                     /gene="hpaG"
                     /locus_tag="ACHKAU_001760"
                     /pseudo
     CDS             117..1447
                     /gene="hpaG"
                     /locus_tag="ACHKAU_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
     gene            1444..2910
                     /gene="hpaE"
                     /locus_tag="ACHKAU_001761"
     CDS             1444..2910
                     /gene="hpaE"
                     /locus_tag="ACHKAU_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5530844.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2912..3763
                     /gene="hpaD"
                     /locus_tag="ACHKAU_001762"
     CDS             2912..3763
                     /gene="hpaD"
                     /locus_tag="ACHKAU_001762"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO5530845.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3773..4153
                     /locus_tag="ACHKAU_001763"
     CDS             3773..4153
                     /locus_tag="ACHKAU_001763"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO5530846.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4264..5067
                     /gene="hpaH"
                     /locus_tag="ACHKAU_001764"
     CDS             4264..5067
                     /gene="hpaH"
                     /locus_tag="ACHKAU_001764"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO5530847.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5078..5866
                     /gene="hpaI"
                     /locus_tag="ACHKAU_001765"
     CDS             5078..5866
                     /gene="hpaI"
                     /locus_tag="ACHKAU_001765"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO5530848.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            6041..7417
                     /gene="hpaX"
                     /locus_tag="ACHKAU_001766"
     CDS             6041..7417
                     /gene="hpaX"
                     /locus_tag="ACHKAU_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO5530849.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7427..8317
                     /gene="hpaA"
                     /locus_tag="ACHKAU_001767"
     CDS             7427..8317
                     /gene="hpaA"
                     /locus_tag="ACHKAU_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO5530850.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8568..10130
                     /gene="hpaB"
                     /locus_tag="ACHKAU_001768"
     CDS             8568..10130
                     /gene="hpaB"
                     /locus_tag="ACHKAU_001768"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO5530851.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10148..10660
                     /locus_tag="ACHKAU_001769"
     CDS             10148..10660
                     /locus_tag="ACHKAU_001769"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO5530852.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            11053..13203
                     /gene="btsT"
                     /locus_tag="ACHKAU_001770"
     CDS             11053..13203
                     /gene="btsT"
                     /locus_tag="ACHKAU_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO5530853.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13334..13537
                     /gene="yjiX"
                     /locus_tag="ACHKAU_001771"
     CDS             13334..13537
                     /gene="yjiX"
                     /locus_tag="ACHKAU_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5530854.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13548..14504
                     /gene="yjiA"
                     /locus_tag="ACHKAU_001772"
     CDS             13548..14504
                     /gene="yjiA"
                     /locus_tag="ACHKAU_001772"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5530855.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14591..14782)
                     /locus_tag="ACHKAU_001773"
     CDS             complement(14591..14782)
                     /locus_tag="ACHKAU_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO5530856.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(14822..15790)
                     /locus_tag="ACHKAU_001774"
     CDS             complement(14822..15790)
                     /locus_tag="ACHKAU_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530857.1"
                     /translation="MENVRFLQGVKQYFCTKALDVILLIYLFLGFILLPYKYLWKDII
                     LSLCFVGILLYALVEENRITEYMGYFVKFSNKNRLNSCAAWCALIACFIFLFLVLSIN
                     IFSVSASLYVFSAFSVFVFLGSIFVILELEFENNNKLILIRSMIIACIPIIYLFASSC
                     ASSLFLSLSNLNITLTPWVEYFWKGMAFMLLFFMLMQLVIYFAFLTLWAKLSVYRLFI
                     LAGAFIVSTILVAFASKNVEGISYYVLKSTIDFEWRNQVKCGELNISRPDERYFGFNT
                     DKYTVFYSNREGKWGVNELKCKKGSDRRDAYSIENVSEYNVPGWLK"
     gene            15931..18363
                     /gene="hsdR"
                     /locus_tag="ACHKAU_001775"
     CDS             15931..18363
                     /gene="hsdR"
                     /locus_tag="ACHKAU_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO5530858.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            18430..19899
                     /locus_tag="ACHKAU_001776"
     CDS             18430..19899
                     /locus_tag="ACHKAU_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5530859.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            19899..21668
                     /locus_tag="ACHKAU_001777"
     CDS             19899..21668
                     /locus_tag="ACHKAU_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5530860.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCKFGLTSEFINGDRGSNYPNKNEYVSQGIPWINTGHIEKNGTLTVTEMNFITEGK
                     FNELRSGKIQKGDLVYCLRGATFGKTAFVIPYETGAIASSLMIIRPFITEMGGYIYNY
                     LTSPFGRSQIYRFDNGSAQPNLSANSVMLYSFPCPPLTEQYRIFSQVGLLHELCDKLK
                     TRIKTAQQTQLHLADALTDAAIN"
     gene            21889..22230
                     /gene="symE"
                     /locus_tag="ACHKAU_001778"
     CDS             21889..22230
                     /gene="symE"
                     /locus_tag="ACHKAU_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO5530861.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(22277..23440)
                     /locus_tag="ACHKAU_001779"
     CDS             complement(22277..23440)
                     /locus_tag="ACHKAU_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO5530862.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(23488..24370)
                     /locus_tag="ACHKAU_001780"
                     /pseudo
     CDS             complement(23488..24370)
                     /locus_tag="ACHKAU_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(24467..24631)
                     /gene="yjiS"
                     /locus_tag="ACHKAU_001781"
     CDS             complement(24467..24631)
                     /gene="yjiS"
                     /locus_tag="ACHKAU_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO5530863.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            24808..26220
                     /gene="yjiR"
                     /locus_tag="ACHKAU_001782"
     CDS             24808..26220
                     /gene="yjiR"
                     /locus_tag="ACHKAU_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5530864.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(26462..27406)
                     /locus_tag="ACHKAU_001783"
     CDS             complement(26462..27406)
                     /locus_tag="ACHKAU_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5530865.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            27873..29105
                     /gene="mdtM"
                     /locus_tag="ACHKAU_001784"
     CDS             27873..29105
                     /gene="mdtM"
                     /locus_tag="ACHKAU_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO5530866.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            29146..30426
                     /gene="yjiN"
                     /locus_tag="ACHKAU_001785"
     CDS             29146..30426
                     /gene="yjiN"
                     /locus_tag="ACHKAU_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO5530867.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            30542..31693
                     /gene="yjiM"
                     /locus_tag="ACHKAU_001786"
     CDS             30542..31693
                     /gene="yjiM"
                     /locus_tag="ACHKAU_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO5530868.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            31703..32470
                     /gene="yjiL"
                     /locus_tag="ACHKAU_001787"
     CDS             31703..32470
                     /gene="yjiL"
                     /locus_tag="ACHKAU_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO5530869.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            32467..32724
                     /gene="ytiA"
                     /locus_tag="ACHKAU_001788"
     CDS             32467..32724
                     /gene="ytiA"
                     /locus_tag="ACHKAU_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO5530870.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            32789..33649
                     /gene="yjiK"
                     /locus_tag="ACHKAU_001789"
                     /pseudo
     CDS             32789..33649
                     /gene="yjiK"
                     /locus_tag="ACHKAU_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            33717..34895
                     /gene="yjiJ"
                     /locus_tag="ACHKAU_001790"
     CDS             33717..34895
                     /gene="yjiJ"
                     /locus_tag="ACHKAU_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5530871.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(34908..35462)
                     /gene="kptA"
                     /locus_tag="ACHKAU_001791"
     CDS             complement(34908..35462)
                     /gene="kptA"
                     /locus_tag="ACHKAU_001791"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO5530872.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            35712..36395
                     /gene="yjiH"
                     /locus_tag="ACHKAU_001792"
     CDS             35712..36395
                     /gene="yjiH"
                     /locus_tag="ACHKAU_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO5530873.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            36392..36853
                     /gene="yjiG"
                     /locus_tag="ACHKAU_001793"
     CDS             36392..36853
                     /gene="yjiG"
                     /locus_tag="ACHKAU_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO5530874.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            36866..38038
                     /gene="iadA"
                     /locus_tag="ACHKAU_001794"
     CDS             36866..38038
                     /gene="iadA"
                     /locus_tag="ACHKAU_001794"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5530875.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            38103..39015
                     /gene="hypT"
                     /locus_tag="ACHKAU_001795"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             38103..39015
                     /gene="hypT"
                     /locus_tag="ACHKAU_001795"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(39008..39400)
                     /gene="iraD"
                     /locus_tag="ACHKAU_001796"
     CDS             complement(39008..39400)
                     /gene="iraD"
                     /locus_tag="ACHKAU_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO5530876.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(39397..39480)
                     /gene="idlP"
                     /locus_tag="ACHKAU_001797"
     CDS             complement(39397..39480)
                     /gene="idlP"
                     /locus_tag="ACHKAU_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO5530877.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(39596..39739)
                     /gene="ytiD"
                     /locus_tag="ACHKAU_001798"
     CDS             complement(39596..39739)
                     /gene="ytiD"
                     /locus_tag="ACHKAU_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO5530878.1"
                     /translation="MADYAEINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         9906 a        10232 c        10109 g         9682 t
ORIGIN      
        1 atagtttgtt aattagatca catttacatc acttactttt gccaaactac gcaatcggga
       61 ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga
      121 aaggcactat cttcgccgta gcgttgaacc atcgcagcca acttgatgca tggcaggaag
      181 cgttccagca atccccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc
      241 gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaggtactga
      301 gcggtgcgac tgttgcgctg attgtgggaa aaacggcgac gaaagtacgt gaagaagatg
      361 cggcagagta catcgccgga tatgcgctgg ctaacgacgt cagcctgccg gaagagagct
      421 tttaccgccc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg
      481 tggctctcag caatgtcgat aatctgacca tctataccga gatcaacggg cgtcctgccg
      541 atcactggaa taccgccgat ttacaacgta acgccgcgca gttgctgagc gccctgagcg
      601 aatttgccac actgaatcca ggcgatgcca ttctgctcgg cacgccacag gcgcgcgtgg
      661 aaatacagcc aggcgatcgc gttcgtgttc tcgcagaagg tttcccgccg ctggaaaatc
      721 cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgcag ccacacccgc
      781 acggcacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta
      841 agccaccgga agaaccgctg gtgttcctga aagcgccaaa taccctcact ggcgataacc
      901 agacctccgt gcgtccaaac aatattgaat acatgcacta tgaagcggag ctggtggtag
      961 ttattggcaa acaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct
     1021 acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac cgccctaacc
     1081 tgcgggtaaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg
     1141 cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc
     1201 aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat
     1261 ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg
     1321 tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt gggctgcctg gcgatgaagt
     1381 agtggtggaa gtagaaggcg tgggctgcct ggtgaaccga attgtgagtg aggaaacagc
     1441 gaaatgaaaa aagtaaatca ttggatcaac ggcaaaaatg ttgcaggtaa cgactacttc
     1501 cagaccacca atccggcaac gggtgaagtg ctggcggatg tggcctctgg cggtgaagcg
     1561 gagatcaatc aggcggtagc ggcagcgaaa gaggcgttcc cgaaatgggc caatctgccg
     1621 atgaaagagc gtgcgcgcct gatgcgccgt ctgggcgatc tgatcgacca gaacgtgcca
     1681 gagatcgccg cgatggaaac cgcggacacc ggcctgccga tccatcagac caaaaatgtg
     1741 ttgatcccac gcgcttccca caactttgaa tttttcgcgg aagtctgcca gcagatgaac
     1801 ggcaagacct atccggttga cgacaagatg ctcaactaca cgctggtgca gccggtgggc
     1861 gtttgtgcgc tggtatcgcc gtggaacgta ccgtttatga ccgccacatg gaaggtcgcg
     1921 ccgtgtctgg cgctgggcaa tactgcggtg ctgaagatgt cagaactctc cccgctgacc
     1981 gctgaccgcc tgggtgagct ggcgctggaa gccggtattc cggcgggcgt gctgaacgtg
     2041 gtacagggct acggcgcaac cgcaggggat gcgctggtcc gtcatcatga cgtgcgtgcc
     2101 gtgtcgttca ccggcggtac cgccaccggg cgcaacatca tgaaaaacgc cgggctgaaa
     2161 aaatactcca tggaactggg cggaaaatcg ccagtgctga tttttgaaga tgccgatatt
     2221 gaacgcgcgc tggacgccgc cctgttcacc atcttctcga tcaacggcga gcgctgcacc
     2281 gccggttcgc gcatctttat tcagcaaagc atctacccgg aattcgttaa acgctttgcc
     2341 gaacgcgcca accgtctgcg cgtgggcgat ccgaacgatc cgaataccca ggttggggca
     2401 cttatcagcc agcaacactg ggaaaaagtc tccggctata tccgtctcgg cattgaagaa
     2461 ggcgcaaccc tgctggcggg cggcccggat aaaccgtctg acctgcctgc gcacctgaaa
     2521 ggcggcaact tcctgcgccc aacggtgctg gcagacgtag ataaccgtat gcgcgtcgcc
     2581 caggaagaga ttttcgggcc ggtcgcctgc ctgctgccgt ttaaagacga agccgaaggc
     2641 ttacgcctgg caaacgacgt ggagtacggc ctcgcgtcgt acatctggac acaggatgtc
     2701 agcaaagtgt tacgcctggc gcgtggcatt gaagcaggca tggtgttcgt caatacccag
     2761 aacgtccgcg acctgcgcca gccgtttggc ggcgtaaaag cctccggcac cgggcgtgaa
     2821 ggcggtgagt acagcttcga agtgttcgcg gaaatgaaga acgtctgcat ttccatgggc
     2881 gaccatccaa ttccgaaatg gggagtctga tatgggtaag ttagcgttag ccgccaagat
     2941 cacgcacgta ccgtcgatgt atctctctga actaccagga aaaaaccacg gttgtcgcca
     3001 gggtgcgatc gacgggcata aagagatcag caagcgttgc cgggaaatgg gcgtcgatac
     3061 cattatcgtt ttcgacaccc actggctggt caacagtgct tatcacatca actgtgcaga
     3121 ccattttgaa ggcgtctaca ccagtaacga actgccgcat tttattcgtg acatgaccta
     3181 caactacgag ggcaacccgg agttggggca gctaattgcc gatgaagcct taaagctcgg
     3241 cgtgcgggca aaagcgcaca atattcccag cctgaaactg gaatacggca cgctggtgcc
     3301 gatgcgctac atgaatgaag acaagcactt caaagtggtc tccatttcgg ctttctgcac
     3361 ggttcacgat tttgccgaca gccgcaagct gggcgaagcg attctgaaag cgatcgaaca
     3421 gtacgacggc accgtggcgg tccttgccag cggttcgtta tcgcaccgct ttattgacga
     3481 tcagcgtgcg gaagaaggga tgaacagcta cacccgcgag ttcgaccgcc agatggacga
     3541 gcgcgtggtg aagctgtggc gcgaaggcca gttcaaagag ttttgcaata tgctgccgga
     3601 gtacgccgac tactgctacg gcgaaggcaa tatgcacgac acggtgatgc tgctggggat
     3661 gctcggctgg gataaatacg acggcaaggt ggagtttatc accgagctat tcccaagctc
     3721 tggcaccggt caggttaacg ctgttttccc gcttcccgcg taaggacgtt ttatgccgca
     3781 ctttatcgtt gaatgcagtg ataacatccg cgaagaagcc gacctgccgg ggttgttcgc
     3841 caaagtgaat ccgacgctgg cagccacggg tatttttccg ctggcgggta ttcgcagccg
     3901 cgtgcattgg gtcgatacct ggcagatggc cgacgggcag catgattatg ccttcgtgca
     3961 tatgacgttg aaaatcggcg caggtcgcag cctggaaagc cgccagcagg cgggtgaaat
     4021 gctgtttgaa ctgattaaaa cgcacttcgc cgccctgatg gagagccgcc tgctggcgtt
     4081 gtcgtttgag attgaagagc tgcatccgac gctgaatttt aaacaaaaca acgtgcacgc
     4141 attgtttaag tagcgcgcag attgcccggt ggcgctgcgc ttaccgggcc tacaaaaccc
     4201 caaaccgtac accgtaggcc ggataaggcg cagccgcatc cggcaatgcc acaggatatc
     4261 gctatgttcg ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa
     4321 cagcgcgaac agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgct
     4381 tacgcggtgc agcgtgaatg ggttcgcctg aaaatcgccg aaggtcgcac gctgaaaggc
     4441 cacaaaatcg gcctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat
     4501 tacggcgcgc tgctggatga catgttcttc cacgatggca gcgatatccc gaccgatcgc
     4561 tttatcgtgc cgcgcattga agtggagctg gctttcgtgc tggcaaaacc gctgcgtggg
     4621 ccaaactgca cgctgttcga cgtctacaac gccacggact acgtgatccc ggcgctggag
     4681 ctgatcgacg ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagttttc
     4741 gacaccattt ctgataacgc cgccaacgcc ggggtgatcc tcggaggtcg tccgatcaaa
     4801 ccagacgaac tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa
     4861 gaaaccggcg tcgccgctgg cgtgctgaat catccggcaa acggcgtggc ctggctggcg
     4921 aacaaactcg caccgtatga cgtacaactg gaagccgggc aaatcattct cggtggttcg
     4981 ttcacccgcc cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg
     5041 ggttccatta gctgccgctt tgtttaagga gagaacgatg gaaaacagct ttaaagcggc
     5101 gctgaaagca ggccgtccgc agatcggatt atggctgggg ctgagtagca gctacagcgc
     5161 agagttactg gccggagcag gattcgactg gttgttgatc gacggtgagc acgcgccgaa
     5221 taacgtgcaa accgtgctca cccagctaca ggcgattgcg ccctacccca gccagccggt
     5281 agtacgtccg tcgtggaacg atccggtgca aatcaaacaa ctgctggacg tcggcacaca
     5341 aaccttgctg gtgccgatgg tacaaaacgc tgacgaagcc cgtgaagcgg tacgcgccac
     5401 ccgttatccc cccgccggta ttcgcggtgt gggcagtgcg ctggcccgag cctcgcgctg
     5461 gaatcgcatt cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat
     5521 cgaaacgcgt gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg
     5581 cgtgtttatc ggcccggcgg atctgagcgc cgatatgggt tatgccggta atcctcagca
     5641 cccggaagta caggccgcga ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc
     5701 gccggggatc ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct
     5761 gtttgtcgcc gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc
     5821 acggtttggt gcgcaggcta cagcgattaa gcccgacgtg tattaatgcc ggatgcgctg
     5881 cgcttatccg gcctacactc gcaccaaacc gtaggccgga taaggcgttt acgccgcatc
     5941 cggcaaaaag ccgtaccaaa tcgcggaccg gataaggcgt ttgtgcgcat ccgccataaa
     6001 ccttgtcgta ccctacaaaa atcccattag aggaagtaaa atgagcgaca cctcacctgc
     6061 cataccggag agtatcgatc cggcgaatca gcataaagcg ctgactgccg gacaacaggc
     6121 ggttattaag aagctatttc gccgcctgat cgtctttctg ttcgtgctgt ttatcttctc
     6181 gttccttgat cgcatcaaca tcggctttgc cggactcacg atgggacgcg acctcggtct
     6241 gagcgccacc atgtttggcc tcgctaccac cttgttctac gccgcttacg tcatcttcgg
     6301 cattcccagc aacattatgc tgagtattgt cggtgcacgg cgctggatcg ccaccatcat
     6361 ggtgctttgg ggcatcgcct ctactgccac catgtttgcc actggcccca ccagcttata
     6421 cgtactgcgt atactggttg gcattaccga agccggcttt ctgcctggca ttctgctgta
     6481 tttaaccttc tggtttccgg cctatttccg cgcccgtgcc aacgccttgt ttatggtagc
     6541 aatgccggta acgacagcgt tgggatcgat cgtttccggc tacattttgt cgctggatgg
     6601 cgtaatggca ttaaaaggct ggcagtggct gtttttgctg gaaggcttcc cgtcggtatt
     6661 actcggcgtc atggtgtggt tctggcttga tgactcaccg gacaaagcta agtggctgac
     6721 gaaagaagac aaaaaatgcc tgcaagagat gatggataac gatcgtctga cgctggttca
     6781 gccagaggga gccatcagcc accacgccat gcaacaacgc agtatgtggc gggagatctt
     6841 cactccggtg gtgatgatgt ataccctggc gtatttctgc ctgaccaaca cacttagtgc
     6901 gatcagcatc tggacaccgc agatcctgca aagctttaat cagggcagca gtaatatcac
     6961 catcggcctg ctggccgccg taccgcagat ttgtaccatt ctcgggatga tctactggag
     7021 ccgtcactca gatcgccgcc aggaacgaag gcatcacacc gcccttcctt atttgttcgc
     7081 tgccgctggt tggttactgg cttcggcaac tgatcacaac atgatccaga tgctggggat
     7141 cattatggct tcgaccggat cattcagcgc aatggcgatt ttctggacaa caccggatca
     7201 gtccatcagc ctgcgggcac gagcgatcgg tattgcggtg atcaacgcca ctggcaacat
     7261 tggttcagcg ttaagtccgt ttatgatcgg ctggttgaaa gatctgaccg gcagctttaa
     7321 cagtggattg tggtttgttg ccacgctgct ggtgattggt gcggggatta tctgggcgat
     7381 tccaatgcag tcctcccgtc cgcgagcgac cccgtaagga acgacgatgt gtgaccgtca
     7441 gattgccaat attgatatca gcaaagagta cgatgaaagc ctgggcacgg acgatgtgca
     7501 ttatcagtcg ttcgcccgca tggcggcctt ttttggccgc catatgctgc cacatcgcca
     7561 cgaacagtac tttcagatgc atttcctcaa tagcggacag attgagctac agcttgacga
     7621 tcatcgctac tcggtggaag cgcccctgtt tgtcctgacg ccgccgtcag tacctcatgc
     7681 gtttattacg gagtctgatg ctgacggtca tgtattgacg gtacgggaag atctgatctg
     7741 gcccctgctg gaagttcttt atccgggcac tcgggaaacc ttcggcctgc cggggatttg
     7801 cctgtcactg gcagataaac ccgacgaact ggcggcgctg gaacactatt ggcaactgat
     7861 agagcgggaa tcggtagaac aactgcctgg tcgggaacac accctgacgt tactggcaca
     7921 ggcagtgttc accctactgc tgcgtaacgc aaaactcgac gaccatgccg ccagcggaat
     7981 gcgcggagaa ttaaaactgt tccagcgttt tcatatgctg attgaaagcc attttcatca
     8041 gcactggaca gtaccggatt acgctaacga attgcatatc accgaatcac gcctcactga
     8101 catctgccgc cgctttgcca accgtccgcc aaaacggttg attttcgaca ggcagctgcg
     8161 agaagccaag cggctgctgc tgttttctga taacgccgtg aacaatattg cctggcaact
     8221 cggttttaag gatccggctt attttgcgcg cttttttaat cgcttagtcg gttgctcgcc
     8281 cagtgcttat cgcgccaaaa aagtacctgt gacgtgaaaa tcccttagcc ttaacgggaa
     8341 accaggcacc acctgctatt cccctttctc cgcctggagc aaggggattt agtaacgtta
     8401 aaaatcgcta aaagcgacct cgatcacaaa accgtacccg atctgaaaag taccagccat
     8461 tgtcccaaaa gtgtcttccc gtaatcgccc ctgcaagctt caattgaata acaaaaacaa
     8521 cacacaaaat taacaatcgt attccgatta atactgtaga ggtcgacatg aaaccagaag
     8581 atttccgcgc cagtacccaa cgtccgttca ccggggaaga gtatctgaaa agcctgcagg
     8641 atggtcgcga gatctatatc tatggcgagc gagtgaaaga cgtcaccact catccggcat
     8701 ttcgtaatgc ggcagcgtct gttgcccaac tgtacgacgc gctacacaaa ccggagatgc
     8761 aggactctct gtgctggaac accgacaccg gtagcggcgg ctatacccat aaattcttcc
     8821 gcgtggcgaa aagtgccgac gacctgcgcc agcaacgcga cgccatcgct gagtggtcac
     8881 gcctgagcta tggctggatg ggccgtaccc cagactacaa agccgctttc ggttgcgcac
     8941 tgggcgcgaa tccgggcttt tacggtcagt tcgagcagaa cgcccgtaac tggtataccc
     9001 gtattcagga aactggcctc tactttaacc acgcgattgt taacccaccg atcgatcgtc
     9061 atttgccgac cgataaagtg aaagacgttt acatcaagct ggaaaaagag actgacgccg
     9121 ggattatcgt cagcggtgcg aaagtggttg ccaccaactc ggcgctgact cactacaaca
     9181 tgattggctt cggctcggca caagtgatgg gcgaaaaccc ggacttcgca ctgatgttcg
     9241 ttgcgccaat ggatgccgat ggcgtgaaat taatctcccg cgcctcttat gagatggtcg
     9301 cgggtgctac cggctcgccg tacgactacc cgctctccag ccgctttgat gagaatgatg
     9361 cgattctggt gatggataac gtgctgatcc catgggaaaa cgtactgatc taccgcgatt
     9421 tcgatcgctg ccgtcgctgg acgatggaag gcggttttgc ccgtatgtat ccgctgcaag
     9481 cctgtgtgcg cctggcagtg aaactagact tcattacggc actgctgaaa aaatcactcg
     9541 aatgtaccgg caccctggag ttccgtggtg tgcaggccga tctcggtgaa gtggtggcgt
     9601 ggcgcaacac cttctgggca ttgagtgact cgatgtgttc agaagcgaca ccatgggtca
     9661 acggtgctta tttaccggat catgccgcac tgcaaaccta tcgcgtactg gcaccaatgg
     9721 cctacgcgaa gatcaaaaac attatcgaac gcaacgttac cagtggcctg atctatctcc
     9781 cttccagtgc ccgtgacctg aacaatccgc agatcgacca gtatctggcg aagtatgtgc
     9841 gcggttcgaa cggtatggat cacgtccagc gcatcaagat cctcaaactg atgtgggacg
     9901 ccattggcag cgagtttggt ggtcgtcacg aactgtatga aatcaactac tccggtagcc
     9961 aggatgagat tcgcctgcag tgtctgcgcc aggcacaaag ctccggcaat atggacaaga
    10021 tgatggcgat ggttgatcgc tgcctgtcgg aatacgacca gaacggctgg actgtgccgc
    10081 acctgcacaa caacgacgat atcaacatgc tggataagct gctgaaataa cgcagcagga
    10141 ggttaagatg caattagatg aacaacgcct gcgctttcgt gacgcaatgg ccagcctgtc
    10201 ggcagcggta aatattatca ccaccgaggg cgacgccgga caatgcggaa ttacggcaac
    10261 ggccgtctgc tcgatcacgg atacaccacc gtcgctgatg gtgtgcatta acgccaacag
    10321 tgcgatgaac ccggtttttc agggcaacgg taagttgtgc gtcaacgtcc tcaatcatga
    10381 gcaggagctg atggcccgcc acttcgcggg catgacaggc atggcgatgg aagagcgttt
    10441 tagcctctca tgctggcaaa aaggtccgct ggcacagccg gtgctgaaag gttcgctggc
    10501 cagtcttgaa ggtgagatcc gcgatgtgca ggcaattggc acacatctgg tgtatctggt
    10561 ggagattaaa aacatcatcc tcagtgcaga aggtcacgga cttatctact ttaaacgccg
    10621 tttccatccg gtgatgctgg aaatggaagc tgcgatttaa gctccagccc cttcacgcat
    10681 tcgtcgaagg ggcattttta ttgtgatctc aatcacatat ccacccgatg aataattctt
    10741 acccaaccgc tcaccgccat tcctgaccgc ttacatccct aaaataacca ctcagttatt
    10801 taccttactt tacgcgtgcg taactctggc aacatcactg caggatagcg gtcaatttac
    10861 ctcctcaaac gcaacgcaaa cctagaacgg cttcggccta tgattaacaa attactctat
    10921 ggattttggt ttgcaggaag gcagcaagtg agtgaatccc cgggagctta taacagtaag
    10981 tgacaggggt gaacgaacgc agctgccgca cctgtaagcc aaaagacgac gagtaaaatg
    11041 ccaggtttta ctatggatac gaaaaaacta ttcaagcaca taccctgggt gattctcgga
    11101 atcatcggtg cattctgcct cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc
    11161 ctgtggatcg tggtcgcctc tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg
    11221 tatatcgccc agaaggtgat gaaactcgac cccacgcgcg cgacgcctgc ggttattaac
    11281 aacgacggtc tgaactacgt tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc
    11341 gctatcgccg gtgctggtcc gctggttggt ccggttctcg ccgcgcagat gggctacctg
    11401 cccggcacgc tgtggctgct ggcgggggta gtgctggccg gtgcggtaca ggactttatg
    11461 gtgctgttta tctcctctcg ccgtaacggc gcatctctcg gtgagatgat caaagaagag
    11521 atgggaccag taccggggac tatcgcgctg tttggctgtt tcttaatcat gatcatcatc
    11581 cttgccgtcc tggcgctgat tgtggttaaa gccctggcag aaagtccgtg gggtgtcttc
    11641 accgtttgct caaccgtacc gattgcgctg tttatgggta tctacatgcg ctttatccgt
    11701 ccggggcgtg tgggtgaagt ctctgtaatc ggtatcgtgc tgctggttgc ctctatctac
    11761 ttcggtggcg tgattgctca cgatccgtac tggggtccgg cactgacctt taaagacacc
    11821 accattacct tcgcgctgat tggctatgcg tttgtttccg cactgctgcc ggtgtggctg
    11881 atcctcgcac cgcgcgacta tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg
    11941 gcgttgggta tcgtggtgct gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt
    12001 gacggtactg gcccgctgtg gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc
    12061 tgtggtgcgg tatctggctt ccacgcgctg atctcttccg gtacgacgcc gaaactgctg
    12121 gctaacgaaa ccgacgcgcg tttcattggc tacggcgcaa tgctgatgga gtccttcgtg
    12181 gcgattatgg cgctggttgc agcgtccatc atcgaaccgg gtctttactt cgcgatgaac
    12241 accccgcctg cgggccttgg catcaccatg cctaacctgc atgaaatggg tggcgagaac
    12301 gcgccgatca tcatggcgca gctgaaagac gttaccgcac acgcggcggc gaccgtcagc
    12361 tcctggggct tcgtgatttc gccagagcag atcctgcaaa ccgcgaaaga cattggtgag
    12421 ccttctgtcc tgaaccgtgc aggtggtgcg ccgacactgg cggtaggtat cgcgcacgtg
    12481 ttccacaaag tgctgccgat ggctgacatg ggcttctggt atcacttcgg tattctgttc
    12541 gaagccctgt tcatcctgac cgcgctggat gcgggtaccc gttctggccg ctttatgctg
    12601 caggacctgc tgggtaactt catcccgttc ctgaaaaaaa ccgactctct ggttgctggt
    12661 atcatcggta ctgcgggctg tgtgggtctg tggggctacc tgctgtatca gggcgtggtc
    12721 gatccgctgg gcggcgttaa gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg
    12781 gcagccgtag cgctggtact gggcaccgtt gtgctgatta agatgaagcg cacccaatac
    12841 atctgggtaa ctgttgttcc ggctgtatgg ctgcttatct gcaccacctg ggcgctgggc
    12901 ctgaaactgt tcagcaccaa cccgcagatg gaaggcttct tctacatggc aagccagtac
    12961 aaagagaaga ttgctaacgg tactgacctg acggcacagc agattgccaa catgaaccac
    13021 atcgttgtga acaactacac caacgcgggc ctgagtattc tgttcctgat tgtggtgtac
    13081 agcatcatct tctacggttt caaaacctgg cttgcggtgc gtaacagcga caaacgtact
    13141 gacaaagaaa caccgtacgt tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac
    13201 taaccggttg ccggatgcgt tgcgcgtatc cggcctacaa agtgtctatc gtaggccgga
    13261 taaagcgttt acgccgcatc cggcaaatcc ctagcccgga ccttccgggc tttacgcata
    13321 tctggaaggc aaaatgtttg gtaacttagg acaggcaaaa aaatatctcg gccaggcggc
    13381 gaagatgttg attggcattc cagactacga caactacgtc gagcatatga agaccaacca
    13441 tcccgacaag ccgtacatga gctatgaaga attcttccgc gaacgccaga acgcgcgcta
    13501 cggcggcgat ggtaaaggcg gtatgcgctg ttgttaatgg agagagcatg aacccgattg
    13561 cagttaccct actcaccggt tttttaggcg cgggcaaaac caccctgctg cgccatattc
    13621 ttaacgaaca acacggctac aagattgccg tgattgaaaa cgaattcggc gaagtctccg
    13681 ttgatgatca actgattggc gaccgcgcca cgcagatcaa aacgctgacc aacggctgca
    13741 tctgctgttc gcgctccaac gagctggaag acgcactact cgacctgctg gataatctcg
    13801 acaagggcaa tattcagttc gaccgtctgg tcattgaatg caccggcatg gccgatcccg
    13861 gtccgattat tcagaccttt ttctcccatg agattttatg ccagcgttac ctgctggacg
    13921 gcgtgattgc gctggtggat gcggtacatg ccgatgagca gatgaaccag ttcaccatcg
    13981 cccagtccca ggttggctac gccgaccgta ttctgctgac caaaaccgac gtcgcaggcg
    14041 aagcagaaaa actgcgtgaa cgcctggcgc gcatcaacgc ccgtgcaccg gtctacaccg
    14101 tcacccacgg cgacatcgac ctgggcctgc tattcaacac caacggtttt atgctggaag
    14161 agaacgtcgt cagcaccaaa ccgcgcttcc actttatcgc ggataaacaa aacgatattt
    14221 cgtcgattgt ggtggaactg gattacccgg tagatatcag cgaagtttcc cgggtgatgg
    14281 aaaacctgct gctggaatcg gcagataaac tgctgcgtta caaagggatg ctgtggattg
    14341 acggcgaacc taaccgcctg ctattccagg gcgtccagcg cctctacagc gccgactggg
    14401 accgcccgtg gggcgatgaa aaaccgcata gcacgatggt gtttatcggg attcagctgc
    14461 cggaggaaga gattcgggcg gcgtttgcgg ggttgaggaa gtaatcgtga aaggttgagt
    14521 gtaaagcagc tcaatgcccc atttagcagt gaggggggaa acagaacgac gtgctcccct
    14581 ccgctaagtt ttattgcgcg tgaatacctg gatctggcaa aaaattgccg cgtttggcgt
    14641 cagaccatgc ttcgctacca gaagcaggaa tgtcgctgta atcaatgtct tcgtcggatt
    14701 tattaaccaa agccatgagt tcagcttctt gctgcacatc aagtgtgtag agtgtgcccc
    14761 gtttatgttt aaccatgctc atttatgacc agaaccttta cgatttaatt ataaagatga
    14821 cttatttcag ccatccaggc acgttatatt ctgagacatt ctcaatggaa tatgcatctc
    14881 tcctgtcaga acctttctta cacttcaact cattgacccc ccatttaccc tctctattgg
    14941 aataaaatac agtatattta tcggtattaa acccgaaata tctttcatcc ggacgtgaaa
    15001 tattcaactc tccacattta acctgattac gccattcaaa atctatggtg ctttttaaaa
    15061 cgtagtacga aataccttct acattttttg aagcaaaagc cactaaaata gtagatacga
    15121 taaatgctcc ggcaagtata aacaacctat atacacttaa tttagcccac agagttaaaa
    15181 aagcaaaata gataacaagt tgcattaaca taaagaaaag caacataaaa gccatccctt
    15241 tccagaaata ctcaacccat ggcgttagtg tgatattgag attactcaaa cttaaaaaca
    15301 aactggaagc acaactactt gcaaacaaat atataattgg tatgcaagct ataatcatgg
    15361 acctaataag tattagctta ttattgtttt caaattccaa ttctaatata acaaaaatac
    15421 ttcccagaaa aacgaatact gaaaaagcac taaaaacgta tagagaggca ctgactgaaa
    15481 aaatattaat tgacaacact aaaaacaaaa atataaaaca cgcaataagt gcacaccagg
    15541 cagcacaact attcaacctg tttttattac tgaacttaac aaaatacccc atatattctg
    15601 taatgcgatt ttcttccact aatgcataaa gaagaatacc aacaaagcac agagataaaa
    15661 taatatcttt ccacagatat ttataaggga gaagtataaa accaagaaat agataaatta
    15721 gcagaatgac atctaatgcc ttagtacaaa aatattgttt cactccttgc aagaaccgaa
    15781 cattctccat atgaatcctt atcctactat tgatgaaact caagaatacc tttaaaacag
    15841 tgacttaacg ctgataatac tctttgaaca gatgaattca tctatttttc tgtgtagaat
    15901 ttgcgcatcg catttagatg agaacacccc atggcggaac tgaacctaag taacctgacg
    15961 gaagcagaca tcattaccaa atgcgttatg ccagccattc tcaatgcagg ctgggacaac
    16021 acaacgcaaa tcagacagga ggtcaaactc cgggacggta aagtcatcgt acgtggtaaa
    16081 gttgcggcac gcagaacggt aaaatctgct gacatcgtgc tgtatcacaa accaggcatt
    16141 cccttagccg tgattgaagc aaaagccaac aaacatgaaa ttggcaaagg gatgcaacag
    16201 ggcattgaat atgcgcgcct gctggacgtt ccttttgttt ttgccaccaa cggggatggc
    16261 tttatcttcc gcgatgccac cgcagccgaa ggtgaatgcc ttgaaaagca aataacgctg
    16321 gatgacttcc cctcccccgc tgaactctgg caaaaattct gcctctggaa aggatacaca
    16381 caagctcagc ttccggtgat tactcaggac tattacgacg atggcagcgg taaatcgcca
    16441 cgttattacc agcttcaggc aatcaacaaa accattgaag ccgtctccaa cgggcaaaac
    16501 cgcgttctgc tggtcatggc gaccggaaca gggaaaacct ataccgcatt ccagatcatc
    16561 tggcgcctgt ggaaatcaaa aaataaaaaa cgcattttgt tccttgccga tcgcaacatt
    16621 ctggtcgacc aaaccaaaaa taatgatttc cagccatttg gtacggcaat gaccaaagtc
    16681 agcggacgca ccattgatcc cgcttatgaa attcacctcg cgctctatca ggctataact
    16741 ggcccggagg aagaccaaaa agcgtttaaa caagtcgcac cagatttctt cgatctgatc
    16801 gtgatcgacg aatgccatcg cggcagcgca tccgaagaca gcgcctggcg agagatcctt
    16861 gattacttca gttccgccac ccaaattggc ttaaccgcca cgccaaaaga gacgcatgaa
    16921 gtctccagca cggattactt cggcgatccg gtttacgtct actcgctaaa agaagggatc
    16981 gaagacggct tcctcgcccc ttataaagtt gtccgtgttg atattgatgt tgatctgcaa
    17041 ggctggcgcc caaccaaagg gcaaactgac ttaaacggcg aagtgatcga cgatcgtatc
    17101 tataaccaga aagatttcga tcgcacgatg gtaatcgacg aacggacaga actggttgcc
    17161 agaaccatta ccgactacct caagcgcacc aacccgatgg ataaaaccat cgtcttctgt
    17221 aacgacatcg atcatgcaga acgtatgcgc cgcgccctgg tgaatctcaa cccggagcag
    17281 gtgaaaaaga acgacaaata cgtcatgaaa atcaccggcg atgatgaaat tggcaaagct
    17341 cagttggata acttcatcaa cccgaaaaaa gcgtacccgg ttatcgcgac cacttcagag
    17401 ctgatgacca ctggtgtgga tgctaaaacc tgcaaactgg tagtactgga ccagaacatc
    17461 cagtcgatga ccaaattcaa gcaaattatc ggtcgtggta cacgcatcga cgaacgttac
    17521 ggcaaactct ggtttaccat cctcgacttt aaaaaagcca ccgaactgtt tgccgatgag
    17581 cgtttcgatg gcattcccga aaaagtcatg gataccacac cagaggatat cgccgatcca
    17641 gaatctgatt ttgaagagaa actcgaagaa atcagcgaac atgacgacga acaggtaaca
    17701 ggcgttgatg aaccgcctgc gccaccatac caggttaccg ataccgatga tgtcggccca
    17761 cttccggaag aagacgagaa gaaaatccgc aagtttcacg tcaacggtgt agcggtgggc
    17821 gttattgcgc agcgtgttca gtattacgat gccgacggta aactggttac cgaatctttt
    17881 aaagattaca cccgcaaaac actgctcaaa gaatatgcct cgctggatga ctttacccgc
    17941 aagtggcagg acgccgatcg caaagaagcg atcattcacg agctggagca acaggggatc
    18001 atctgggaag tactggcaga agaagtcggt aaagatctcg acccgttcga catgctttgc
    18061 cacgtagtgt atggtcagcc gccgttaacc cgcaaagagc gcgccgagaa cgtgcgcaag
    18121 cggaactact tcacaaaata ctctgaagca gcgcaagccg tgctcgataa tctgctggat
    18181 aaatacgccg atgcgggcgt acaggagatc gaaagtattc aggtgctgaa acttaaacca
    18241 ttcgacagca tgggcacttt accggagatt attaaaaccg gatttggcga ccgtaacggg
    18301 tataatcagg cgctcagcga gctggaaaac gaaatctacc aattaccgcc ccgctctgct
    18361 taacaactct cttttgtgcc ttctgcaacc aacatgttgc agaaggcctt gtttttcatg
    18421 gaatacaaca tgtctatcag ttcagtaatc aaatcattac aagatattat gcgcaaagat
    18481 gccggtgtgg atggcgatgc gcagcgtctc ggtcagctct cctggctgct gtttttgaaa
    18541 atcttcgatg cccaggaaga ggtgctggaa ctggagcagg ataactatca atatccgatc
    18601 ccacagcgtt atttatggcg cagttgggcc gcaaacgctc agggcattac cggtgattcc
    18661 ctgctggaat tcgttaatga tgatctgttc ccggcgttga aaaacctcac tgcgcctatc
    18721 gataaaaacc cacgcggcta cgtggtgaag caggcgttca gcgatgccta taactatatg
    18781 aaaaacggta cgctactgcg ccaggtgatc aacaagctga acgaaattga ctttaccagc
    18841 gccagcgaac gccatctgtt tggtgatatt tacgaacaga tccttaaaga tctgcaatct
    18901 gcgggcaatg ctggcgaatt ctatacgcca cgcgccgtca ctcgctttat ggtggatcgc
    18961 gttgatccga aactcggcga atccattatg gacccggcct gcggtacggg cggttttctt
    19021 gcctgcgcat ttgatcatgt aaagaacaaa tacgtgaaga gcgtcgccga tcatcagacg
    19081 ctgcaacaac agatccacgg tgttgagaaa aaacagcttc cgcacctgct ggcgaccacc
    19141 aatatgctgc tgcacggcat tgaagtaccg gtacaaattc gtcacgacaa caccctgaac
    19201 aaaccgcttt cctcctggga tgaacaactg gatgtcattg ttaccaaccc gccgtttggt
    19261 ggcacggaag aagacggtat tgagaagaac tttccggcag agatgaaaac ccgcgaaacg
    19321 gcggatctgt tcctgcaact gattgtggaa gtactggcga aaaacggtcg cgcggcggtg
    19381 gtattgccgg atggcacact atttggcgaa ggcgttaaaa ccaaaatcaa aaaactgctt
    19441 accgaagagt gcaacctgca taccatcgtg cgtttaccga atggggtgtt taacccctat
    19501 accggcatta aaaccaacct gttgttcttt accaaaggtc agccaaccaa agagatttgg
    19561 ttctatgagc atccgtatcc ggcgggcgta aaaaactaca gcaaaaccaa gccgatgaag
    19621 tttgaagagt ttcaggcgga gatcgactgg tggggtaacg aggccgatgg ttttgccagc
    19681 cgcatcgaga atgagcaagc gtggaaagtc agcattgatg acgtgattgc gcgtaacttc
    19741 aatctggata ttaaaaaccc acatcaggcg gaaaccatta gccatgatcc ggacgaactg
    19801 ttagcgcagt atgcaaaaca gcaggcggag atccagacgc tgcgtaatca actgcgcgat
    19861 attcttggcg ctgcgctgtc tggcaaggag gttaactaat gagtgtggaa aagctgatcg
    19921 ttgatcatat ggaaacctgg acctcggcgt tgcaaacccg ttccaccgcc gggcgcggca
    19981 gctccggtaa gattgatttg tatggtatta agaaattgcg tgagctgatt ctggaactgg
    20041 cggtgcgagg taaactggtg ccgcaggatc cgaacgatga accggcgtcg gagctgctga
    20101 agcgtattgc ggcggaaaaa gcagagctgg tgaagcaagg taaaattaaa aagcaaaaac
    20161 cgctgccgga aattagcgag gaagagaagc cgtttgaatt gccggagggg tgggaatgga
    20221 ctcgattaat taatcttggt atatgggctt tagggtctgg ttttccaaat gtggtacagg
    20281 gaagcactga taaagaaatt ttgatgtgca aagttagcga catgaatctt gaaggtaacg
    20341 agaaattcat tttttcgaca aaaaatacaa tttcaaaaga cctagctgat gaatataaaa
    20401 ttaaaatctc agaacccggc accatcattt tccctaaaat tggcggagca attgcaacta
    20461 ataaacgaag aattttagtt caggatacag ctattgataa caactgtctt ggaataaaac
    20521 catgtgatgc aatatcgggg gagtggttct atctaatcct taatactctc gatatgtcga
    20581 agtatcaatc aggaacctca atacccgcga taaatcaaag tgtcataggt agtataccaa
    20641 ttgcgcttcc ttctttaaaa atgcaagaga agattgtttc ttatgtcata accctaatgt
    20701 ccctttgcga ccaactggaa cagcaatccc tgaccagtct ggacgcacat cagcaactgg
    20761 ttgaaaccct gttgggaaca cttacagaca gccaaaacgc cgaggaactg gctgaaaact
    20821 gggcgcgtat tagcgagcat ttcgacaccc tatttaccac cgaagccagc gtggatgcgt
    20881 taaaacagac cattctgcaa ctggccgtaa tgggcaaact tgtgccgcag gatccgaatg
    20941 acgaaccagc ctctgaactg ctcaaacgaa ttgcgcagga aaaagctcaa ctggtgaaag
    21001 aaggaaaaat aaaaaaacaa aaaccgttgc cgccaattag cgatgaggaa aaaccgtttg
    21061 aacttccgga agggtgggag tggtgtaaat ttggtttaac atcagagttt ataaatggcg
    21121 atcgtggaag taattacccc aataaaaatg agtatgtttc tcaaggtatt ccttggatca
    21181 atactggaca tatagaaaaa aatggtacac ttacggttac agaaatgaat tttataaccg
    21241 aaggaaaatt taatgaatta agaagtggga aaattcaaaa aggtgatttg gtttattgcc
    21301 tacgaggagc aacttttggt aaaactgcct ttgtaatccc ctatgaaaca ggagctattg
    21361 cctcatcact aatgatcatt agaccattta tcacagaaat gggagggtat atttataatt
    21421 atttaaccag tccatttggg cgctcacaga tttaccgctt tgataatggc tcagcacaac
    21481 ccaacctatc agcaaatagt gtaatgttgt attcattccc atgcccacca ttaactgaac
    21541 aatatagaat attttcacaa gtaggtttac ttcatgaact atgcgataag cttaaaactc
    21601 gtataaaaac agcccaacaa acccaactgc accttgcaga cgcactcact gacgcggcga
    21661 taaactaatt ttctcgccct caaaaaacca ataaagcggc taaatggccg ctttattcac
    21721 atcaccaaaa attatatttc acgctaattt tcatcttatt ttcattaatt tttagctgga
    21781 ctttccctgt atttactgat gatatatcca ggtatttagc gcggtgcgga tgtgcgccaa
    21841 cacacccgca ccgctaatca caatcactat tatcggagaa tagcagttat gactgacacg
    21901 cattctattg cacaaccgtt cgaagcagaa gtctccccgg caaataaccg tcaattaacc
    21961 gtgagttatg cgagtcgcta cccggattac agccgtattc ccgccatcac cctgaaaggt
    22021 cagtggctgg aagccgccgg ttttgccacc ggcacggcgg tagatgtcaa agtaatggaa
    22081 ggctgtattg tcctcaccgc ccaaccaccc accgccgaag agagcgaact gatgcagtcg
    22141 ctgcgccagg tgtgcaagct gtcggcgcgt aaacaaaagc aggtgcagga ttttattggg
    22201 gtaattaccg gtaaacagaa agtcgcctga taaattgacc cggtagcgcc agtgctaccg
    22261 ggtattattc gccgcgttat accaacgcgc tctccatctg tttttcaaat gcctgacgaa
    22321 ttaatcccat aatgtaagga agttgtgcga ggtcgctgaa accaatttct acatcgccat
    22381 taccccagcg cccaacattt gtcacatctt tggcaatacc tttcggatcg accagttcat
    22441 gaaactgcat attaagcgac aaccgcagtc ggcttttttg cggtacaaca tcaacaaaat
    22501 tcgtttcagc tttaaacgca atatagagct tcaatacttc ctgcgtgatc cctgcgtcca
    22561 ggcgcataac ttcatcgcga agatgatcga ataacacccg gctatgcgaa ccatcagcaa
    22621 gaaaaggata atcactcagg ctgtaactgg ttttgttttc aggctgcccc cgataaacag
    22681 ctaaaacctc ttcagaaaga gaaggcgctt gccagacctg aaccgcgaga tcggccagac
    22741 gatcggcacg ggcgcgaatg gcggcttcat cccatttttc gcactgccca agaccgatat
    22801 tcaaatagag cgggctatgt ttaaagccgt cttcaatatc gcgtttttcc gcgaaaggtc
    22861 tgtcactgta gcgggagtta tagcgcgtta aagtgagatt ccccaacgta tgtaacagtt
    22921 ctttatgcac acgctgccag tcgcttccca gctcttcacg ccatttagcc gacagatttt
    22981 cgttctgcgg cataatgtgc tcaatcgtaa actcttccac gcgctctctg cgtttatcgt
    23041 tttccagtct tcgtaaccag tagctgcggc tgcggaagtt atagagatcg cgaactttta
    23101 attcccgttt aaattcatca tcgttgggga aacgacgata tgaaggcagg ttcataaaat
    23161 gtacctgaat actttccaga tatttttctt tattaatgac cttataaaaa gtggcaaacg
    23221 tcttgttcaa agaattcgtc ggaattgcac atactgcacg gcggaaaaca taagattcaa
    23281 ttaaacgaat tatgctcagg aaatcttcgt gagacaaatc gtcattttta tagtcatgat
    23341 aaagcgccag taagaaagga tacgccacat caacctttaa ctcgcgcaaa tcctgaaaag
    23401 ccgtagcaag cgatttgtca ctttcttttc ccaatgccat tgcacagtaa tactccgcgt
    23461 agatatgaat atcttcaacc agccgatcta cgcctttttc agcaacactc tggctgcggg
    23521 catggagttt aaatgcctca tagacttcat ctgtccgagg gatctccccc gtttttaccg
    23581 tcagataatg acgcataaaa ctgtcaaaat attcgctgta accttgctga ccaaaggcga
    23641 cttccattgg acgccagtga tcttcataca accgggtttg atgctctggt tcgaggccca
    23701 tcagaataaa attgcgcacc agatcggcct gacttaacgc cttaccggtg gagttcatac
    23761 tttcaaaaat cagttgcgga ttatcctgac cacgattaag cgccacatcg acaattaata
    23821 actttgctaa cccacgacat aaggggatca agtcatcacc gagtttggca atttgttcat
    23881 caaagaaagt aaagttttcc attattcgat gggaatagtt ttctggcatt ggtctttgtt
    23941 ttattaacgc cagtaaactg tctttatcag tctcggtcag tagtaattta aaacctttct
    24001 cgccggactc ataaggattg agcaaataat aattacgcaa tttcattgct gaaaagccat
    24061 caaaaacttc gtcttcgcca agatggcgcg ataaagcctc aatcagcaac attgcggtcg
    24121 tcagccgttg ttgaccatca atgaccagta acggagaaat accagaaacc tgatacaacc
    24181 cctgctcaat ataaacaacc gaaccgataa aatgcgctga tatatcgtca cgctttccgg
    24241 cacgaatgat gtcgtcccaa agttgccgac attgttgttc tgtccatgaa taagtccgtt
    24301 gataaatggg aataacaaac tgctgcgaac gttttaaaaa atcgagtagg cgggcttctg
    24361 tagctttcat tctgacgtct ttccgtgaag cgatgatttc gcaataataa ccacttcctt
    24421 ttggaggtta tagagaaatc ctgtttatcc gcgatgaatt gccccctcac tccacatcct
    24481 ccctgcgtaa cccaatatct ctcaaccgct catcactcat ctgctgtaac actcgtcggg
    24541 tctgcatttg ccgccgccaa cgcctcaccg cctgccagag ttgcacaagt ctgataaacg
    24601 gcgttttggc tttgttttcg tgaaattcca tctgctgcct cctcatcttt cagaggcttc
    24661 atcatccacc ttctggcagt tcacaataca gatgcaaata ttagttttat ttaacataca
    24721 gacaggttaa aacagcatct gcatccggtt ttttagcctc atctgtaccg gtttttcttt
    24781 ctgtatggtc caaataagga atacagcatg acgcgttatc aacatctggc gactctactt
    24841 gccgagcgga ttgagcaagg gctgtatcgt catggggaga aattgccgtc ggtgcgtagc
    24901 ttaagtcggg agcacggcgt cagcatcagc accgtgcagc aggcgtatca gacgctggag
    24961 acgatgaagc tcataacccc acagccgcgt tcgggttatt ttgtcgcaca acgtaaagcc
    25021 cagccgccag tgccgccgat gacgcgtccg gtgcagcgcc cggtggaaat tacccagtgg
    25081 gatcaggtgc tggatatgct ggtggcgcat agcgacagtt ccattgttcc gttaagcaaa
    25141 agcacgccgg atgtcgaaac gcccagcctg aaaccgctct ggcgggagct aagccgggtg
    25201 gtgcagcata atctgcaaac cgtgctcggt tatgacttgt tagccggtca gcgggtattg
    25261 agagagcaga ttgcccgcct gatgctcgac agcggctcgg tggtcaccgc cgatgacatc
    25321 attatcacca gcggctgcca taactcgatg tcgctggcgt taatggcggt gtgtaaaccg
    25381 ggcgatattg tcgcggtcga atctccctgt tattacggtt cgatgcagat gctgcgcggc
    25441 atgggcgtga aagtgattga aatcccaacc gatccagaaa caggcatcag cgttgaagcg
    25501 ctggaactgg cgctggaaca gtggccgatt aaaggcatca ttctggtgcc aaactgtaat
    25561 aatccgctgg gatttattat gccggacgca cgcaaacggg ccgttctctc tctcgctcag
    25621 cgtcatgata ttgtgatttt tgaagatgat gtctacggcg aactggcaac ggagtatccg
    25681 cgcccgcgga ccatccattc atgggatatc gacgggcgag tgctgttgtg cagctcgttc
    25741 agtaaaagta ttgctccagg cctgcgcgtg ggttggggcg caccggggcg ttatcacgat
    25801 aaactgatgc atatgaaata cgccatcagc agctttaatg tgccgtccac gcaaatggcg
    25861 gcggcaacgt ttgtgctgga aggtcactat catcgccata tccggcggat gcggcagaac
    25921 tatcagcgca atttggcgct ttatacctgc tggatacggg aatattttcc ctgcgaaatc
    25981 tgtattacgc gcccgaaagg cggattttta ctgtggatag aattgcctga acaggtcgat
    26041 atggtctgcg ttgcgcggca gctgtgccgc atgaatatcc aggtggcagc aggctcgatt
    26101 ttctcggctt ccggcaaata ccgtaattgt ctacgcatca actgcgcttt gccgctcagc
    26161 gaaacctatc gcgaagcact aaagcaaatt ggcgaggccg tgtatcgggc aatggaataa
    26221 attcgaatta gtggcgaagc acctgatttc ctctcacgaa tcgcctgtaa ctcgttgtat
    26281 ctttgcaggg tcatcgccac gttccagcat ggcttaggca aagtgtgctt cgttcatacc
    26341 ggatgcggcg tgaacgcctt atccggccta caaaccgcgc aaattcaata gattgcagag
    26401 gaaatgtagg tctgataagc gtagcgcatc aggcagcttt gcgtttgtca tccgtctctg
    26461 attagtattc gatccatgga tcgcgcttga tttcaggcaa gggctgctct aatgcctgca
    26521 tttgctctgt cgaaagtccg gtaatctttt gtatccattc aggatctgcc ccattctgca
    26581 acaacaatct aaggaggcgt tgttcccctt cttctttccc cttttccatt cccaacagcc
    26641 atccatcatt ataaattcgc tctgcaatcg tcattattcg ctccctgtgt tgtggcattc
    26701 gacgggtaag ttcgctgata aacttcttaa aacgcgcttc atcgccagtc agtaaaatgt
    26761 aatttaacag cgcagttatc tggctgtcat tagcacactc tgtaaccagt aatactacca
    26821 gttggtcgat aagccccatc agatcgcgct ggcgaatatg cttttggatc aactccaaca
    26881 gggcgactct gcgatgctgc acaatctcgt cgtctggcac gacagtgata tccaccagcg
    26941 ggaacgcggc ggtataaagt ttccgtgcgg tagttgggtc ggcaaattcg tccagccagc
    27001 acagggacca gggataagga ctacggctac cgtgataaaa tagcatcggt ataaccagcg
    27061 gtagcgcccg acgtttatca tgctcgatat ggcgctgcat caccgccatg gaatagcgca
    27121 tcaggcgaaa ggccatatga atatcctcac ggctctgatg ttcaatcacc acataaatat
    27181 agccatcgcc ttcgcgggta tttaccgacc acagaatatc ggagtgtagc gcccgcaatt
    27241 tttcatcgac aaagctggcg gattccagtt ttaagctgtc gagatcgcac agttcacgta
    27301 aatctttggg taaatagatc tccataaaat cccgcgcggt gtcagggtgg gtgagaaagg
    27361 atttaaataa tgcgtcatgc ggcgtgctgg ttgtgaagtt tgtcatggcg atccgtcacc
    27421 tgttgataag atggcccgac tttagcgact aacaaaacca ttcacacctg gcaatttccc
    27481 gttccccgag gcgctttcca ggctattttt gttcctctgc aagcgataca tttttccgga
    27541 aacaggctcg cgttttacat ttcctgtacc cggtagatat tcccctttcc accgcgctct
    27601 tttgattaat cgcaaatttt aattacactt aaggtgtata ttttctatgc aaaccatcaa
    27661 ttcaagaggt gtaatgtgct gatgactatt tgtaatcgtt atacattctg acccgaactc
    27721 agaaggtatt tctctgtctg tataagcaca ggcagtgtgg ttgattacat gaaattcagt
    27781 acatttgtcc ttctcgccta cctttcgtca ttaccgacgg tattgaattt cgttttcccc
    27841 gttggggttc tccggacaag gagttgtttg ttatgccacg tttttttgcc cgccatgccg
    27901 ccacgctgtt tttcccgatg gcgttgattt tgtatgattt tgccgcgtac ctgtcgacgg
    27961 acctgattca gccggggatc attaatgtgg tccgtgattt taatgccgat gtcagtctgg
    28021 ccccggcagc cgtcagtctc tatctcgcgg gcggtatggc gttacagtgg ctgctggggc
    28081 cgctttccga caggattggc cgcaggccgg tgctgattac cggggcgcta atatttaccc
    28141 ttgcctgcgc tgcgacaatg ttcacaacgt ctatgacaca gtttcttatc gcgcgtgcaa
    28201 ttcagggcac cagtatctgt tttattgcta ccgttggtta tgtcacggtg caggaggcgt
    28261 tcggacagac aaaagggatc aagttgatgg cgattatcac ctccatcgta ctgattgcgc
    28321 cgattattgg cccgctttcc ggcgcagctc tgatgcactt tgtgcactgg aaagtccttt
    28381 ttgccatcat tgcggttatg ggttttatct catttgttgg cttactgctg gcgatgccag
    28441 agacggtgaa gcgcggcgcg gttccattta gcgccaaaag cgtcttgcgc gattttcgta
    28501 atgtcttttg caatcggctg ttcctctttg gcgcagcaac catctcttta agctatatcc
    28561 caatgatgag ttgggtggct gtctcgccgg tgatcctgat tgacgcaggc ggcttaacaa
    28621 cttcgcagtt cgcctggaca caggttccgg tgttcggcgc ggtgattgtc gcgaatgcca
    28681 tcgtggcgcg tttcgttaaa gatccgaccg aaccgcgctt tatctggcgt gccgtaccca
    28741 ttcaactggt cggcctcgca ctgttgatta tcggcaatct gctgtcaccg cacgtctggc
    28801 tgtggtcggt gctgggcacc agtctgtatg ctttcgggat tggtttaatc ttcccgacct
    28861 tattccgctt tacacttttt tccaataact taccgaaagg gaccgtctcc gcatcgctga
    28921 atatggtgat cctgatggtg atgtctgtct cggtcgaaat cggccgctgg ctgtggttta
    28981 acggcggtcg cttgccgttt catctgttag ccgtcgtagc gggagttatc gtcgttttca
    29041 ccctggcggg gttgctcaat cgcgtgcacc aacatcaggc tgccgagctg gcggaggagc
    29101 ggtgattttt gcgcgatcct gccgtcaggc tctattctta acgttatgaa taaactcatt
    29161 gaactcagac gcgccaaaat gttggcgctc tctttactgc ttatcgccgc tgctaccttt
    29221 gtcgttacgc tgtttttgcc acccaatttt tgggtgagcg gcgtgaaggc aattgctgaa
    29281 gcggcgatgg tcggcgcgct ggcggactgg tttgcggtgg tggcgctgtt tcgccgcgtg
    29341 ccaattccga tcatttctcg tcatacggcg attatcccgc gtaataaaga ccggattggc
    29401 gaaaatctcg gtcagttcgt gcaggaaaaa tttctcgata cccagtcgct ggtggcattg
    29461 attcgacgtc acgaaccggc gttgctaatt ggtaactggt ttagccagcc agaaaacgcc
    29521 cgccgcgttg gtcagcatct gttgcagatc atgagtggtt ttcttgaact gaccgatgat
    29581 gcacgtattc agcgcctgct taagcgcgcg gtccatcggg cgattgataa ggtcgatctt
    29641 tccggaacca gtgcgttgat gctggagagt atgaccaaaa acgatcgtca tcaggtgctg
    29701 ctcgatacgc tgatcgcaca gttgattgct cttctccagc gcgataaatc gcgcaagttt
    29761 atcgcccaac aaattgttcg ctggctggag agtgagcatc cactgaaagc caaaattctc
    29821 cccaccgaat ggctgggcga acatagcgcg gagttggttt ctgacgcggt gaattctttg
    29881 cttgatgata tcagccgcga tcgtgcgcat cagatccgcc atgcatttga tcgcgccact
    29941 tttgccctga tcgacaagtt gaaaaacgat ccggaaatgg cagcgcgagc tgatgccgta
    30001 aaaagctatc tgaaagagga tgaagccttt aaccgctatc tcagtgaatt gtggggggat
    30061 ttacgggaat ggctgaaagc ggatatcaac agtgaagatt ctcgtgtgaa agaacgtatc
    30121 gcgcgggcgg gtcaatggtt tggcgaaacg ttaattgccg atgatgcctt gcgggcgtcg
    30181 ttaaatggtc atctggaaca agccgcgcac cgcgtcgcac ctgagttttc cgcattcctg
    30241 acgcgccaca tcagcgatac ggtaaaaagc tgggatgccc gggatatgtc gcggcaaata
    30301 gagttaaata tcggcaaaga tctgcagttt atccgtgtca acggtacgct ggttggcggt
    30361 tgtattgggc taattttata tttgctgtca cagctcccgg ccttgttccc cctcggcaat
    30421 ttttagaaat ccatgaaaaa ctaagtggct aaccgcgaga gagatcaaat aaaccacact
    30481 aaaagaatgg taataatgtg gcctcttttc attatctccc gtggtacggg gaaggaaaat
    30541 catgtcactt gtcaccgatc tacccgccat tttcgatcag ttctctgaag ctcgccagaa
    30601 aggctttctc accgtcatgg atctcaagga gcgcggcatt ccgctggttg gcacttactg
    30661 cacttttatg ccgcaagaga tcccgatggc agccggtgcg gttgtggttt cgctctgttc
    30721 cacctctgat gaaaccattg aagaagcgga gaaagatctg ccgcgcaacc tctgcccgct
    30781 gattaaaagc agctacggct tcggtaaaac cgataaatgc ccctacttct acttttctga
    30841 tctggtggtc ggtgaaacca cctgcgacgg caaaaagaaa atgtatgaat acatggcgga
    30901 gtttaagcct gttcatgtga tgcagctgcc aaatagcgtt aaagacgatg cctcgcgtgc
    30961 gttatggaaa gccgagatgc tgcgcttaca aaaagcggtg gaagaacgtt ttgggcacga
    31021 aattagcgaa gatgctctgc gcgatgccat tgctctgaaa aaccgcgaac gtcgcgcact
    31081 ggctaatttt tatcatcttg ggcagttaaa tcctccggcg cttagcggca gcgacattct
    31141 gaaagtggtt tacggcgcaa ccttccggtt cgataaagag gcgttgatca atgaactgga
    31201 cgcgatgacc gcccgcgttc gtcagcagtg ggaagaaggc cagcgactgg acccgcgtcc
    31261 acgcatttta atcaccggct gcccgattgg cggcgcagca gaaaaagtgg tgcgcgcgat
    31321 tgaagagaat ggcggctggg ttgtcggtta tgaaaactgc accggggcga aagcgaccga
    31381 gcaatgcgtg gcagaaacgg gcgatgtcta cgacgcgctg gcggataaat atctggcgat
    31441 tggctgctcc tgtgtttcgc cgaacgatca gcgcctgcaa atgctcagcc agatggtgga
    31501 agaatatcag gtcgatggcg tagttgatgt gattttgcag gcgtgccata cctacgcggt
    31561 ggaatcgctg gcgattaaac gtcatgtgcg tcagcagcac aacattcctt atatcgctat
    31621 tgaaacagac tactccacct cagatgtcgg gcagctcagt acccgtgtcg cggcctttat
    31681 tgagatgctg taaggagtgg cagtgacata ttcgattggc attgattctg gttctaccgc
    31741 caccaaaggg atcttactgg cagacggcgt gattacgcgc cgtttcctcg ttccaacacc
    31801 ctttcgtccg gcaacagcaa ttactgaagc ctgggaaact ctgcgcgaag ggttagagac
    31861 aacgccgttt ctgacgctca ccggctatgg acggcaactg gtggattttg ccgataaaca
    31921 agtgacggaa atctcctgtc atgggctggg cgcacggttt cttgcgccag caacgcgcgc
    31981 ggtaatcgac atcggtggtc aggacagcaa agtgattcag cttgatgatg acggtaacct
    32041 gtgcgatttc ctgatgaatg acaaatgcgc ggcgggcacc gggcgtttcc tggaggtgat
    32101 ctcgcgcacg cttggcacca gcgtcgagca actcgacagc attaccgaaa atgtcacgcc
    32161 gcacgccatc acgagtatgt gcacagtgtt tgctgaatca gaagcgatca gcctgcgctc
    32221 agcgggcgtc gcgccagaag cgattctcgc cggagtgatt aacgcgatgg cgcggaggag
    32281 tgccaatttc attgctcgtc tctcctgtga agcgccgatt ctgtttactg gtggcgttag
    32341 tcattgccag acgtttgccc ggatgctgga atctcatctg ggaatgccgg taaataccca
    32401 tcctgatgcg caatttgctg gcgcaattgg cgcggcggta attggtcaac gagtgaggac
    32461 acgccgatga aagagttttt atttttattt cactccacag tcggcgtcat acaaacccgc
    32521 aaagcgttgc aggcagcggg catgactttt cgcgtcagcg atattccccg tgatttacgc
    32581 ggcggctgcg ggttatgcat ttggctgacc tgtccccccg gcgaggaaat gcaatgggtg
    32641 atccctgggc tgactgagtc aatttattgt cagcaggatg gtgtctggcg ctgcattgca
    32701 cattaccggg tttctccgcg ttaaggattc attaaggtcg ctacgtatac ttctcgatat
    32761 cttattgatt tgccttaaag ggattaccgt gacaaaaagt atttcgttaa gtaaacgcat
    32821 ctccgttatt gtgattcttt ttgccattgt ggcagtgtgt actttcttcg tgcaatcttg
    32881 cgcccgtaaa agcaatcatg cagcataatt tcagaattac cacgccacca ttgacggcaa
    32941 agaaattgca ggcataacga ataatatctc ttcgctgacc tggtcggcac aaagcaatac
    33001 tctttttagc actatcaaca aacctgccgc cattgttgaa atgaccacca atggtgattt
    33061 tatccgcaca attcctctgg attttgttaa ggatctcgag acgatcgaat acattggcga
    33121 taatcagttt gtcattagcg atgagcgcga ttacgccatt tatgtgattt cactgacccc
    33181 aaactcggaa gtaaaaatcc tcaaaaaaat aaaaatcccg ctgcaagact ccccaaccaa
    33241 ttgcggtttt gaagggttgg cttattctcg ccaggatcac actttctggt tttttaaaga
    33301 gaaaaatccg attgaggtct acaaagttaa cgggctgtta agtagcaatg aattacacat
    33361 cagcaaagac gaggcactac aacggcaatt tactttagat gatgtgtccg gcgcagaatt
    33421 taaccagcaa aaaaatacgc ttctggtgct gtctcatgaa tctcgcgcac tccaggaagt
    33481 aaccctggtt ggcgaggtta tcggtgaaat atcactaacc aaaggtagtc gaggtttgtc
    33541 gcataacatc aaacaggccg aagggattgc gatggatgcc tctggtaata tttatatcgt
    33601 tagcgaacca aatcgttttt atcgctttac ccctaaatca tcgcattaac aggctggcat
    33661 atttcacctt tttgttatta acttgttaac aacaaaaatg acatacaagg ttaaacatgc
    33721 cttcgtcctc gcatcccgta gaacgctttt ctttcagcac cgcgcttttc gggatgctgg
    33781 ttctgacctt aggtatggga ttaggtcgct tcctttatac gcctatgttg cccgtcatga
    33841 tggcggaagg ggcgttttca tttagccagc tctcgtggat tgccagcggc aactatgcgg
    33901 ggtatctggc tggcagtctg ctattttcgt ttggcgcatt tcaccagccg tcgcgcctgc
    33961 gcccatttct gttggtttct gccctggcga gcgggttgtt gatcctcgca atggcatggt
    34021 tgccgccatt tattctggtg ttattgattc gcttcctggc gggtgtcgcc agcgccggta
    34081 tgctgatttt tggttcgacg ctgattatgc agcacacgcg ccatcctttt gtgctggcag
    34141 ctttgttttc tggcgttggc attggcattg cactgggcaa cgaatatgtt ctggcaggcc
    34201 tgcattttgc cttatcctcg caaacgttat ggcaaggcgc cggggcgctt tctggcatga
    34261 tgctgattgc acttacgctt ttaatgccct cgaaaaaaca cgccatcgca ccaatgccat
    34321 tggcaaaaac ggagcaacag ataatgagct ggtggttact ggctattctg tacggcctgg
    34381 cgggatttgg ttatatcatc gtcgcgactt acctgccgct catggcgaaa gatgcaggct
    34441 caccattgtt aaccgcccat ctctggacgt tagtcggctt atcaattgtg ccaggctgct
    34501 ttggctggct atgggcagca aaacgttggg gggctttgcc ttgcctgacg gcgaatttgc
    34561 tggtgcaggc tatcagtgtg ctgcttactc tcgccagcga ctctcctctc ttgcttatca
    34621 ttagtagtat cggttttggc ggtaccttta tgggcacgac atctctggtg atgactatcg
    34681 cccgccaact gagcgttccg ggaaatctca atcttttagg ctttgtgacg ctcatttacg
    34741 gcattggaca aatcctcggc ccggcgctga ccagtatgct cggcaatgga acatcggcgc
    34801 ttgccggcgc cactctctgc ggcgcggcgg cgttgtttat tgctgcgctt atcagcacag
    34861 tacaattatt taagctgcaa gtggtcactt catgaggagt gtttccctta ccactcaaga
    34921 aattcgaccg caaccgttga tgtcagccag accccgtttt ccgtttgcca gaaagggatg
    34981 cctcgtttcg ccatttcctg tgctttgacg gttaagataa ccggcgatcc gtgtcttgct
    35041 cccactttgc gtgccgttgc ttcatcagca gagagatgta cgtagtgacg ctcacctgca
    35101 atcagcccct gtttttttat ctcgtcaagg aaacggcttg ccgtaccgtg gtaaagaaac
    35161 tgtggcggtg ttttttcggc aaaggaaata gcgacctgcg aagtcaaatg cccctgaacc
    35221 gcgcggatgc acctaccatc actggaataa ctaaaacgct ttttatcact ggtggcgacc
    35281 acggtatcca gcaatgtacg ggttaatcgc ttgcccgctt tttgggcaca aagtatgagc
    35341 ttatcgatat ccgcccatcc ttcacggtca agtacgatac caatcgcctc cggtttgtgt
    35401 cggaggacaa aacttaaaaa cttgctcgta tccgccagct ctttttcgtt gtactttgcc
    35461 atctcgtttt cgtccctgga ttttctgctt ttatttactg atgtcattgt cactgggttt
    35521 atgaattttc tgcaactcaa tcctgcgcag agccttttcc acacagcgta atttttccgc
    35581 tatgcaaaaa acgcatagcc agaaacatac ttgcattttc cccataccca catggccgat
    35641 aaggtttttc agctccagga attttcctgg ctcaagcaac acacaaacac aaaaaaacaa
    35701 tggggattgt tatgacacaa caaggggatg ctgtagccgg ggatctggcg acagaaaaag
    35761 tgggcattaa agggtatctg gctttttttc tcaccataat ctttttttcc ggtgtttttt
    35821 ccgggacaga cagctggtgg cgcgtttttg atttttccgt tctgaacgga tcgtttgggc
    35881 aactgcctgg tgcgaacgga gccaccacct catttcgcgg ggcgggtggc gcgggggcta
    35941 aagatggctt tctctttgcg ctggagctgg caccgtcagt cattctctct ctggggatta
    36001 tttcaattac cgatggtctt ggtggattac gcgccgcgca gcagttaatg acacctgtgc
    36061 taaaaccgtt gttgggcatt ccagggatct gctcgctggc gctgatcgct aacctgcaaa
    36121 acactgatgc cgccgccggt atgaccaaag agctggcgca ggaaggggaa attaccgaac
    36181 gcgataaagt gattttcgcc gcatatcaaa ccagcggtag cgccatcatc accaactact
    36241 tctcttccgg cgtcgcggtg ttcgcctttc tgggcacgtc cgtaatcgta cctttagccg
    36301 tcatcctggt gtttaaattt gtcggagcca acattttgcg cgtctggctt aacttcgaag
    36361 aacgtcgcaa cccaacgcaa ggagcgcaag catgacaact caggtccgca aaaacgtcat
    36421 ggatatgttt atcgatggcg cgcgccgtgg ctttactatc gccacgacca acctgctgcc
    36481 aaacgtggtg atggcgtttg tcatcattca ggcgctgaaa attaccggcc tgctcgactg
    36541 ggtggggcat atctgtgaac ccgtaatggc gctgtgggga ttacccggtg aagcggcaac
    36601 cgtcctttta gccgcattaa tgagtatggg tggcgcagtc ggtgttgccg ccagtctggc
    36661 aaccgctggc gcattaaccg gtcacgatgt caccgtttta ctgcccgcga tgtatttaat
    36721 gggcaacccg gtgcaaaatg tcggtcgctg cctggggaca gccgaagtga atgccaaata
    36781 ttacccgcat atcattacgg tctgcgtgat taacgcattg ctgtcgatct gggttatgca
    36841 gcttattgtt taacaaggag ttaccatgat tgattatacc gcagccggtt ttaccctgct
    36901 gcagggagcg catttgtatg cgccggaaga tcggggaatt tgcgatgtcc tcgtcgctaa
    36961 cggcaaaatt atcgccgttg ccagcaatat cccttctgac attgtaccgg actgcacggt
    37021 tgtcgatctc agcgggcaga tcctctttcc tggttttatt gatcaacacg tccatttgat
    37081 tggtggtggc ggtgaagcag gtcccacgac gcgcacgcca gaagtggcgc taagtcgcct
    37141 gacggaagcg ggcgtcacgt cagtggttgg tctgctgggc accgactcta tctctcgcca
    37201 cccggaatcc ctgctcgcca agacccgtgc gctcaatgaa gaaggcatca gcgcctggat
    37261 gctgaccggc gcttatcatg tcccttcccg caccattaca ggttccgtgg aaaaagacgt
    37321 ggcgattatc gatcgtgtga ttggcgtgaa atgcgccatc tctgatcacc gttctgccgc
    37381 accggacgtt tatcacctgg ctaatatggc ggcggaatcc cgcgttggcg gtttgctcgg
    37441 cggtaaacct ggcgtcaccg tgttccacat gggcgacagc aaaaaggcgt tacagcctgt
    37501 ctatgacctg ctggaaaact gcgatgtgcc gatcagcaag ttgctgccga cccacgttaa
    37561 ccgcaacgtt ccgttgtttg agcaggcgct ggagttcgcg cgcaaaggcg gcactatcga
    37621 tatcaccagc agcattgacg aaccggtcgc ccctgccgaa ggtattgccc gcgccgttca
    37681 ggcgggtatt ccgctggcac gcgtcaccct cagctccgac ggcaacggta gccagccgtt
    37741 cttcgatgac gaagggaatt taacccatat cggtgttgcc ggttttgaaa cgttgctgga
    37801 aaccgtgcag gtgctggtca aagactatga tttcagtatc agcgatgccc tgcgcccgct
    37861 caccagtagc gtagccggtt tccttaacct gaccgggaaa ggcgaaattc tgccaggcaa
    37921 tgatgctgac ttgctggtca tgacgccaga actgcgcatt gagcaggtat acgctcgcgg
    37981 caaactgatg gtcaaagacg gcaaagcctg cgtgaaagga acgtttgaaa cggcttaaac
    38041 ggttgtcagc cgcccgaatg gacgtagaat gcccgcaagg gcggctgaca gagtaaaacg
    38101 taatggatga ctgtggtgcg attttgcata atattgaaac caaatggctt tatgattttc
    38161 tgaccctgga aaaatgccgc aatttttccc aggcggcagt cagtcgcaac gtctcgcaac
    38221 cggcattcag ccgccgcatc cgtgcgctgg aacaggcgat tggtgttgaa ttgtttaacc
    38281 gccaggtaac gccgctgcaa ctctcagaac aagggaaaat cttccattcg cagatccgcc
    38341 atctgttgca acagttagag agcaatctgg cagagctgcg tggcggcagc gattacgcgc
    38401 aacgtaaaat caagatagcc gctgcacact ctctttccct cgggctgtta ccgtccatta
    38461 tcagccagat gccgccgctc tttacctggg caattgaagc tattgatgtc gatgaagcgg
    38521 tcgataaact gcgtgaaggg caaagtgact gtattttttc ctttcacgac gaagatttgc
    38581 tggaagcgcc gtttgaccac attcgcttat ttgaatctca attgttccct gtctgcgcca
    38641 gtgacgaaca cggagaagca ctttttaacc tcgcgcagcc acactttccg ttactgaatt
    38701 acagccgcaa ctcctacatg gggcgattga ttaatcgcac cctgacgcgc cacagtgagt
    38761 taagtttcag cacctttttt gtctcttcga tgagcgagct tttaaagcag gttgccctcg
    38821 acggctgtgg gattgcctgg ctgccggaat acgctatcca acaagaaatt cgcagcgggc
    38881 agctcgttgt gctgaatcgg gacgaactgg tgatcccgat ccaggatcta cgcataccgg
    38941 atgaatactc gcatgaatcc cgttgccgaa cgcttctggc gtgaactgcg cgagctggag
    39001 attgtgctta gctgacattc tccagcatcg cactacacca accgccatta tcccagacca
    39061 gatcaaacgt tagcggttca ccgtcgtggt acagcattat ctcaaggctg atcgccagag
    39121 tgtggcagta cgcttcttta aaattaacct gcaacgccgc aatccgtggc tcatgaaata
    39181 acagtgcaga acgcagctga cggcaatacc agctctggag atgtttgtcg ctctggttat
    39241 gcacttgcca ctcgggcatg cctgaaacaa gtaatgacgg taatgacgaa tgaggagaat
    39301 taaaaagagt gaggagatcg ctgcataccg atttacgcag caatgacgtt ttattgcctg
    39361 aaatttcagg taaaacagcc tgacgtgatt ggcgcatcat tttgcgcact cctgacgttt
    39421 agcaacatat gcggcttgcc ctgaacaccg ggcaccactg tattgatgct cattttccat
    39481 agccattccc ttttgttcat tacatccatg tcagtctgag acaggcctga atcctgtcag
    39541 caaagtataa aaccttactt gcctatgaat atctactgac ttttcaggaa aaaagtcatg
    39601 atttgataaa aatcatcaaa ctcaaaaaaa aaccgctagt atattctgaa tattccgaat
    39661 agttacgtag atgaaaatac ttgtcaccac cgctacttaa ttccggtgtg aaattattta
    39721 tttccgcata atcagccatg tattatttga aacaattagt ttgtaaataa tataaattga
    39781 taacatatca aatacatcaa aaataccatt cacaggcatt attaatatag acatcaatat
    39841 agcaacacca aattccaatt agtaataaga tatgctttta aattgcatga aaataatttc
    39901 aaatgagcgt tttttgatac tttagagtg
//
LOCUS       DAZFJF010000031        39194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFJF010000031 DAZFJF010000000
VERSION     DAZFJF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.031"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..310)
                     /gene="yfgG"
                     /locus_tag="ACHKAU_001799"
     CDS             complement(119..310)
                     /gene="yfgG"
                     /locus_tag="ACHKAU_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO5530879.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            328..480
                     /locus_tag="ACHKAU_001800"
     CDS             328..480
                     /locus_tag="ACHKAU_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5530880.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            662..2905
                     /gene="pdeF"
                     /locus_tag="ACHKAU_001801"
     CDS             662..2905
                     /gene="pdeF"
                     /locus_tag="ACHKAU_001801"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO5530881.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2944..4485)
                     /gene="ppx"
                     /locus_tag="ACHKAU_001802"
     CDS             complement(2944..4485)
                     /gene="ppx"
                     /locus_tag="ACHKAU_001802"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO5530882.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4490..6556)
                     /gene="ppk1"
                     /locus_tag="ACHKAU_001803"
     CDS             complement(4490..6556)
                     /gene="ppk1"
                     /locus_tag="ACHKAU_001803"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO5530883.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6727..7365)
                     /gene="purN"
                     /locus_tag="ACHKAU_001804"
     CDS             complement(6727..7365)
                     /gene="purN"
                     /locus_tag="ACHKAU_001804"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO5530884.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7365..8402)
                     /gene="purM"
                     /locus_tag="ACHKAU_001805"
     CDS             complement(7365..8402)
                     /gene="purM"
                     /locus_tag="ACHKAU_001805"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO5530885.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8727..9353
                     /gene="upp"
                     /locus_tag="ACHKAU_001806"
     CDS             8727..9353
                     /gene="upp"
                     /locus_tag="ACHKAU_001806"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO5530886.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9439..10728
                     /gene="uraA"
                     /locus_tag="ACHKAU_001807"
     CDS             9439..10728
                     /gene="uraA"
                     /locus_tag="ACHKAU_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO5530887.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10823..11524
                     /gene="hda"
                     /locus_tag="ACHKAU_001808"
     CDS             10823..11524
                     /gene="hda"
                     /locus_tag="ACHKAU_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO5530888.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11662..12021)
                     /gene="arsC"
                     /locus_tag="ACHKAU_001809"
     CDS             complement(11662..12021)
                     /gene="arsC"
                     /locus_tag="ACHKAU_001809"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO5530889.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12042..13505)
                     /gene="bepA"
                     /locus_tag="ACHKAU_001810"
     CDS             complement(12042..13505)
                     /gene="bepA"
                     /locus_tag="ACHKAU_001810"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO5530890.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13718..14779
                     /gene="yfgO"
                     /locus_tag="ACHKAU_001811"
     CDS             13718..14779
                     /gene="yfgO"
                     /locus_tag="ACHKAU_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5530891.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14817..15665)
                     /gene="focB"
                     /locus_tag="ACHKAU_001812"
     CDS             complement(14817..15665)
                     /gene="focB"
                     /locus_tag="ACHKAU_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO5530892.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15687..17699)
                     /gene="hyfR"
                     /locus_tag="ACHKAU_001813"
     CDS             complement(15687..17699)
                     /gene="hyfR"
                     /locus_tag="ACHKAU_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO5530893.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17729..18142)
                     /gene="hyfJ"
                     /locus_tag="ACHKAU_001814"
     CDS             complement(17729..18142)
                     /gene="hyfJ"
                     /locus_tag="ACHKAU_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO5530894.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18135..18892)
                     /gene="hyfI"
                     /locus_tag="ACHKAU_001815"
                     /pseudo
     CDS             complement(18135..18892)
                     /gene="hyfI"
                     /locus_tag="ACHKAU_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18889..19434)
                     /gene="hyfH"
                     /locus_tag="ACHKAU_001816"
     CDS             complement(18889..19434)
                     /gene="hyfH"
                     /locus_tag="ACHKAU_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO5530895.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(19444..21111)
                     /gene="hyfG"
                     /locus_tag="ACHKAU_001817"
     CDS             complement(19444..21111)
                     /gene="hyfG"
                     /locus_tag="ACHKAU_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO5530896.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21101..22681)
                     /gene="hyfF"
                     /locus_tag="ACHKAU_001818"
     CDS             complement(21101..22681)
                     /gene="hyfF"
                     /locus_tag="ACHKAU_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO5530897.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22686..23336)
                     /gene="hyfE"
                     /locus_tag="ACHKAU_001819"
     CDS             complement(22686..23336)
                     /gene="hyfE"
                     /locus_tag="ACHKAU_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO5530898.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23348..24787)
                     /gene="hyfD"
                     /locus_tag="ACHKAU_001820"
     CDS             complement(23348..24787)
                     /gene="hyfD"
                     /locus_tag="ACHKAU_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO5530899.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24804..25751)
                     /gene="hyfC"
                     /locus_tag="ACHKAU_001821"
     CDS             complement(24804..25751)
                     /gene="hyfC"
                     /locus_tag="ACHKAU_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO5530900.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25762..27780)
                     /gene="hyfB"
                     /locus_tag="ACHKAU_001822"
     CDS             complement(25762..27780)
                     /gene="hyfB"
                     /locus_tag="ACHKAU_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO5530901.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27780..28397)
                     /gene="hyfA"
                     /locus_tag="ACHKAU_001823"
     CDS             complement(27780..28397)
                     /gene="hyfA"
                     /locus_tag="ACHKAU_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO5530902.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28650..29120)
                     /gene="bcp"
                     /locus_tag="ACHKAU_001824"
     CDS             complement(28650..29120)
                     /gene="bcp"
                     /locus_tag="ACHKAU_001824"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO5530903.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29120..29692)
                     /gene="gcvR"
                     /locus_tag="ACHKAU_001825"
     CDS             complement(29120..29692)
                     /gene="gcvR"
                     /locus_tag="ACHKAU_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO5530904.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29838..30716
                     /gene="dapA"
                     /locus_tag="ACHKAU_001826"
     CDS             29838..30716
                     /gene="dapA"
                     /locus_tag="ACHKAU_001826"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO5530905.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30733..31767
                     /gene="bamC"
                     /locus_tag="ACHKAU_001827"
     CDS             30733..31767
                     /gene="bamC"
                     /locus_tag="ACHKAU_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO5530906.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31980..32693
                     /gene="purC"
                     /locus_tag="ACHKAU_001828"
     CDS             31980..32693
                     /gene="purC"
                     /locus_tag="ACHKAU_001828"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO5530907.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32847..33074
                     /gene="vapB"
                     /locus_tag="ACHKAU_001829"
     CDS             32847..33074
                     /gene="vapB"
                     /locus_tag="ACHKAU_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO5530908.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            33074..33472
                     /locus_tag="ACHKAU_001830"
     CDS             33074..33472
                     /locus_tag="ACHKAU_001830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO5530909.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33619..34482
                     /gene="ypfJ"
                     /locus_tag="ACHKAU_001831"
     CDS             33619..34482
                     /gene="ypfJ"
                     /locus_tag="ACHKAU_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO5530910.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34497..36512
                     /gene="tmcA"
                     /locus_tag="ACHKAU_001832"
     CDS             34497..36512
                     /gene="tmcA"
                     /locus_tag="ACHKAU_001832"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO5530911.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36586..37284
                     /gene="ypfH"
                     /locus_tag="ACHKAU_001833"
     CDS             36586..37284
                     /gene="ypfH"
                     /locus_tag="ACHKAU_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5530912.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37394..37594)
                     /gene="ypfN"
                     /locus_tag="ACHKAU_001834"
     CDS             complement(37394..37594)
                     /gene="ypfN"
                     /locus_tag="ACHKAU_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO5530913.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37622..38749)
                     /gene="dapE"
                     /locus_tag="ACHKAU_001835"
     CDS             complement(37622..38749)
                     /gene="dapE"
                     /locus_tag="ACHKAU_001835"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO5530914.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            complement(38753..39109)
                     /gene="yffB"
                     /locus_tag="ACHKAU_001836"
     CDS             complement(38753..39109)
                     /gene="yffB"
                     /locus_tag="ACHKAU_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO5530915.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9592 a        10031 c        10295 g         9276 t
ORIGIN      
        1 caggacaaag acgtaaaatt taagatgaaa gaatatctgc cgctttgata attccaaaga
       61 gcaaaagcat ccgacagcga agtgccgtta ggttctgtga aagtggagaa ggtaaccgct
      121 aacgttgtac cggtgattcg acggagagtg tggattgctg agcttctttt ttgctctgat
      181 ggtgctgcca ggcaccgacg gacgagtaga taaaacggcc aaagaagaag ataaagctga
      241 tgagcagtac gatacgggtc atgcgactgt taaatcggtg tcgttttcgc atactggttg
      301 cctgactcac aaaaggttcc ttgaagtatg tcccacgctg tggacggtac ttacattaag
      361 gcacaacagg acaaaatggt caattcttcg ttatgtaaaa aagcgtcagt ggatacatat
      421 tttaatgtta tggaagttaa tttaattatt tacaatgatg atgtaataat gatgaattaa
      481 catgataaac gtaataattc attaatcagg gttattttgt ttgatatata tcaattggaa
      541 ctttcatatg acacttagaa tcaatgctct ctatagtgat gaataatcat cattcgaagt
      601 caggtgggat gcctgtctga atacacctcc ttcaggatgt gggggattct gctgagcatc
      661 tatgaaactg aatgcaactt atataaaaat acgtgataaa tggtgggggc ttccgctgtt
      721 cctgccttct ttaatcttgc ccattttcgc ccacattaat actttcgcgc atatttcttc
      781 cggtgaggtt tttctctttt atctgccttt ggcactgatg atcagcatga tgatgttttt
      841 cagctgggcg gcattgccag ggatcacctt agggattttt gtccgcaaat atgcagagct
      901 gggtttttac gaaacgctct cattaacggc taattttatt atcattatca ttctctgttg
      961 gggcggttac agggtcttta ctccccggcg taacaacgtt tcacatggtg atacccgttt
     1021 aatttcccag cgtatattct ggcagattgt gtttcctgca acgctgtttc tgatactttt
     1081 ccagtttgct gcatttgtag gattactggc gagcagagaa aatctggtcg gtgtcatgcc
     1141 ttttaacctc gggactttaa tcaattatca ggccttactg gtgggtaatc tgatcggtgt
     1201 cccgctgtgc tacttcatca ttcgggtagt gcgaaatcca ttttatttac gtagctatta
     1261 ttcgcaatta aaacagcagg ttgatgccaa agtcaccaaa aaagagttcg cgctctggct
     1321 actggcatta ggtgctttat tgttgctgtt atgcatgccg ttaaatgaaa aaagcacaat
     1381 ttttagcacc aattatacct tgtcattatt gctgcccctg atgatgtggg gagcgatgcg
     1441 ctatggttat aagctgattt cgctgctctg ggcggtcgtg ttgatgatca gcatccacag
     1501 ctatcaaaat tacattccca tttatcctgg ctataccacg cagctgacca taacctcctc
     1561 cagttatctg gtattctctt ttattgtcaa ttatatggct gtactggcaa ctcgtcagcg
     1621 agcggtagtc agacgcattc agcggcttgc gtatgtggac ccggtggttc atctgccaaa
     1681 tgttcgcgcc ctgaatcgcg cgttgcgtga tgccccctgg tctgcgcttt gttatttacg
     1741 catccctggc atggaaatgc tggttaagaa ctatggcatc atgctgcgga ttcaatacaa
     1801 gcaaaaactt tctcactggc tgtcaccctt gctggaaccg ggtgaagatg tttatcagct
     1861 ttcgggtaac gatctcgcgc tgcggctgaa tacagaatcg caccaggagc gcattaccgc
     1921 actggatagc catctcaagc aatttcgttt cttttgggat ggcatgccga tgcaaccgca
     1981 gattggcgtc agttactgct atgtgcgctc gccagtgaat catatctacc tgctgctggg
     2041 agagctaaat acggtcgccg aactttccat cgtgaccaac gccccggaaa atatgcagcg
     2101 tcgcggggca atgtatttgc aacgcgaatt gaaagataaa gtcgcgatga tgaatcgact
     2161 acagcaggcg ctggaacaca accatttttt cctgatggcc cagccgatta ccggtatgcg
     2221 tggtgatgtt taccatgaaa ttcttctgcg catgaaaggt gagaatgatg aactgatcgg
     2281 ccccgatagc ttcttgccgg tcgcgcacga atttggttta tcgtcgagta tcgacatgtg
     2341 ggtcattgag catacgctgc aatttatggc tgaaaacaga gcgaagatgc ccgctcaccg
     2401 ttttgctatt aatctgtctc caacctcggt atgtcaggct cgttttcctg ttgaagtcag
     2461 tcagttgctg gataaatatc agattgaagc gtggcaactt atttttgaag tcaccgaaag
     2521 taatgctctg accaatgtta agcaggcgca aatcaccttg cagcatcttc aggaattagg
     2581 ctgccagatt gcgattgatg atttcggcac cggctacgcc agctatgcgc ggcttaaaaa
     2641 tgtgaatgcc gatctgctta aaattgacgg cagttttatc cgcaatattg tgtcaaatag
     2701 tctggattat cagatagtgg catcgatttg ccacctggcg cgaatgaaga aaatgcgggt
     2761 agtggcagag tacgttgaaa acgaagagat ccgcgaggcg gtgctctctt tggggatcga
     2821 ttatatgcag ggttatctta ttggtaagcc gcaaccgtta attgatacgc tgaatgaaat
     2881 cgaacccatt cgcgaaagtg cctgaataat gcgggccgac atttctcgtc ggcccgcaaa
     2941 gcattaagcg gcgatttcag gtgtactttc ttcttcaatt ttcaaccgcc agccagccac
     3001 gccttcccag tattcttgct ccttttccag atcaagcagt accagcgcat tctgactaaa
     3061 ccagtcatgc gggaaacgca gtgtccagtg actgtcatca gtaatcagtg tcaatgttgg
     3121 cggtgtggtg gttgcctgac gttgattgtt tagtaatacg ccaaggcgca atagctgtat
     3181 cagtggcagg aactgtttct tcttaaacaa ggtaaagcgc ggcagatcgt cgagcttaat
     3241 cgctttacgg tgatagcgca ccagtgtcgc catcatcagc tgctgttcct gattaaaacc
     3301 cggcaagtca ctgttttgca gaatataagc ggagtggcga tgcaaaccgc tgtggttgat
     3361 attcaacccg acctcatgca gcatggcggc ccatcgcagt agcgcctcca gttgcggatg
     3421 cgccagcttc ggttgctgtt cccgccactg ttcgtacatt tgcatagtgg tatccagcac
     3481 tcgtcgggcc tgttcgctgt cgatgtgata ctggttggcg aggctgctgg cggtgcgact
     3541 acgcacatcc tgatgacgga aacgtccttc catttcatac agtacgcctt cgcgtaacgc
     3601 cccgtcagaa aggcgcagtt cacggatggc taaagcatca aacacaccac ataaaatcgc
     3661 cagtccagga acgaagactg ttttccgctc ttcggaaaga cccggtaaac tcagcgatgc
     3721 gaaattacgg tgacgtaaaa cttcttttac cagtttttcc agacgttccg gggtaattat
     3781 cccgtctttc tcgcccattt ccattaatac ttcatgggcg gcttttatgg taccggaagc
     3841 gcccattgcc acgttccagc cctgaatacg gaattgccag gttaaagttt ccagtttttg
     3901 tgctgccgcc atgcgagcgc gctgaaaatt ctctttattg atgaccccgc cagggaaata
     3961 aagctgggca aagctgacac aacccatccg gcggctttca acgagaatag gttcgaaatt
     4021 ttcaccaatc accagttccg tagatccgcc gccaatatca ataaccagtt tgcgaccttt
     4081 ttccggttgg gtatgttcca cgcccataaa aatcagacgg gcttcttcat taccggaaat
     4141 aatttcaatc gggtagggaa tgaccttttc cgcgcgtttc agaaagtcag tggcgttcag
     4201 cgcctgacgc agtgtatggg tgccaactat acagacgctg gcaggagaaa acccttgtag
     4261 ccgttcggca aacagcgaca gacagttcaa accgcgcgtc attgcctctt cactcaacat
     4321 attatctggc cccaggccgt ccgccagatg cacccgctgt ttcaggcggc caataatctg
     4381 catggcacca tctaccacac gggctatgac catgtgaaaa ctgtttgaac caagatcgac
     4441 cgcagcaaac tcctgcggac gaggggattt atcgtgtatt ggcatagggt tattcaggtt
     4501 gttcgagtga tttgatgtag tcataaatcg ccaactgcgc ccgtactttg cggcgattgc
     4561 cgcggggaac gtagcgatta ctgagttctt tatcgatata acgtgctttg accgtatcgc
     4621 tgaacaatat gtcgatgatg tccagcaccc gctgcttcag gcgcggatcg agcagcggtg
     4681 tcgccacttc aatacgataa tcaatattgc gcgtcatcca gtcggcggaa gaaaggtaga
     4741 cctttttatc gccgccattt tcaaaaatat aaacccggtc atgttcaagg taacggtcaa
     4801 caatactgat ggcacgaatg ttgtcgctaa tgccttccag attggggatc agcgaacaca
     4861 ttccgcgaac cagcagatta accggtacgc cggagctgga ggccgcatac agacgatcaa
     4921 ccaggccttt atcgacaagg ttatttagct tcagggtgat accactgggc agcccttgct
     4981 gcacgttggc gatttcgcgg tctaccattt catataacag acggcgagag ttttgcggtg
     5041 acaccattaa ataatcaaat gtcactgggc ggtatgggtt ttcaataaag ttaaataccc
     5101 gccgtacttc gttggtgata cgcgcatctg cggtcagcaa cgaatagtca gtataaagac
     5161 gcgcggtttt ttcgttaaag ttcccggtcc cgatgtgtgc gtaacggacc acttcaccgt
     5221 tttctttacg tgaaatcagg aacagtttgg cgtgaatttt cagccccggc gcagagaaga
     5281 taacgtgcac gcctgcttcg gtcaggcgct tcgcccagtg aatgttggct tcttcgtcga
     5341 aacgcgcctg taactcaacc accacagtga ctttcttacc gttatgtgcg gcgtggatca
     5401 tcgagtcgat gatgcgtgaa tctttcgcca cgcggtaaat gttaatttta atcgccagta
     5461 cgctcgggtc gaacgaagcc tgacgcagca gttccagcac atgctcaaag gtgtgataag
     5521 gataatagag caacacatcg cgttcgcgaa tggcatcaaa accattgcgg aactgggctt
     5581 tatcaaacca aatgtggcgt aaacgcggca gtggtttgtt caccagattg gctttgccga
     5641 cattggggaa attaataaag tctttaaaat tatgataacg accgccgggg acgatggagt
     5701 cgtagcggga aatagtcagt ttttcgcgta acacttcaac cagcgcattg ggcatatcgc
     5761 gctgataaac aaaacgcacc ggctcagcag ttaaacgctg cttgagactg gaagacatca
     5821 actccatcag gctggcttcc atctcatgca ctaaatcgta ttcggcatcg cgtgtcatct
     5881 tcattgaata ggcattcagc gcgtcataat caaagaagcc tttgaaaata tcatcaaggc
     5941 agtaacgcag aatgttatcc agaagaatca tcggcttgcg tcgacgcggc gcttctggcg
     6001 gtaaattcac aaagcgcggc actttatctg atgggatctc cagcagcgcg taacggatgg
     6061 tatcaccacg gataatttcc accgccagat aggtgtaatc atccttcagg aactgcacta
     6121 agtcagtgtc aggattgatt aaaatcggcg taatgtgctg acgcagatac tgcttaaaat
     6181 aatgacgaag ccagttttgt tgattgacgg agagctggcg ttcattaatc aggaagatct
     6241 ggttgcgcgc catctccagc aatagctcgt tgtagaggcc gtcgaattcc tgatcggctt
     6301 tcagcacccg ggactgaatt ttgcccagta aatggcggga atgagagttg gagccttgtt
     6361 cttcgctaat aatgatgcgt cgcttcagtt cagcgaagcg gactttatag aactcatcaa
     6421 ggttattgga atagatcccc aggaaacgca tcctttcaat cagcgggtta gatttgtccg
     6481 ccgcttcctg aagcacgcgt tcattgaacg ataaccaact gagctctttt tcgatgtata
     6541 gcttttcctg acccattacc acttttactc cgttttattc acgggacact gcctggatat
     6601 tatggcgagc aatattggca gatgtccaac tatgccagaa aagggggaaa aaaactgctg
     6661 gagcttgcga tttacaggtg aattgaaaaa cgccagcggc agagctggcg ctttaactac
     6721 ggggcattac tcgtcggcag cgtagccctg cggcggcaga cgttgaccat ccagccacgc
     6781 ggcgttttcg tgcattttca gacgaccatc ggcaaaccag ctaatcacca gtggataaat
     6841 agcgtgttcc tgggtttgca cgcgggcggt gatgtcatct tccgtatcac cagcaaatac
     6901 cgggactttc gcctgtaaaa taaccgggcc accgtccagt tcatcggtga cgaaatgcac
     6961 cgatgtaccg tgctcttcat caccattttc cagcgcttga cgatgggtgt gtaatccggg
     7021 atatttcggc agcagagaag ggtgaatgtt cagcaaacgc ccggcatagt gggagacaaa
     7081 cgccgggctg agaatgcgca taaaaccagc cagcacgacc acatcgggtg cgtacatgtc
     7141 gatttcatga atcaactccc ggtcataggc ttcacgactg tcaaacgcgc tggcgatgag
     7201 cgtatgcgtt gcaatacccg cctggcgggc gcgttcaagg ccgaacgcgt cggccttatt
     7261 gctgaaaact gcccgtacgg tgcctttaat tttgttggtt ttacaggcgt caataattgc
     7321 ctgtaaatta cttccgttac cggaaataag caccacaata ttcattattc gataaccacg
     7381 cgttgttcgg aatcagaggc tttgatgata ccgattttcc acgcgttttc accgttggca
     7441 ttgagcaggg cgagggcttt gtccacttcc ggagcaggca gggcaataat catcccgacg
     7501 ccgcagttga aggtgcgata catttcatgg cgctcaacgt tacctgccgt ttgcagccag
     7561 ttgaacactt ccggccactg ccaggaagat tcatcaatca ctgcctgagt attatctggc
     7621 aatacgcgtg gaatgttttc ccagaagccg ccgccggtca ggtgcgcaat ggcatgcaca
     7681 tcgacctttt caatcaactc cagcactgac ttcacgtaaa tgcgggtcgg tgccagcaga
     7741 taatcggcta atggcttacc atcaagttgg gtggtttgcg gatcacaacc gctgacttca
     7801 agaattttgc gcaccagcga atagccgttc gagtgcggac cgctggaacc gagtgcaatc
     7861 agcacatcgc cgtcgctgac tttagagccg tcgatgattt ctgatttttc taccacgcca
     7921 acgcagaaac ccgcgacatc gtaatcctca ccgtgataca tccccggcat ttctgccgtt
     7981 tcgccaccca ccagtgaaca gcccgattgc agacatcctt ccgcgatacc gctgatcacc
     8041 gctgaagcgg tatcaacatc cagttttccg gttgcgtaat agtcgaggaa aaacagcggc
     8101 tctgcacctt gcaccaccag gtcattaacg cacatggcga ccagatcaat accaatggtg
     8161 tcgtgacgtt ttaagtccat tgccagacgc agcttggtac ctacgccgtc agtgccggaa
     8221 accagcacgg gttcacgata tttttgcggc aatgcacaca gcgcaccgaa gccgcccaga
     8281 ccgcccatca cttccggacg acgcgttttc ttcactacgc ctttgattct tccaaccaga
     8341 gcattacccg cgtcaatatc aacaccggca tctttgtagc taagagaggt tttatcggtc
     8401 actgcttggg tccccacgcg ttacttgcgg tagaaaaata aaattcggcg caattctaac
     8461 agggaaagca aacgtttgcg agactgcttt acacaacctt tttgcacgtc ttttgcccag
     8521 gcgcgcggcg aaagaagact tgtaccaggg taaaggttag ttttcggatg gaataatctt
     8581 ctttcataac catttgaata taaaataact ttatctcaag ccgttatcat tttgactaaa
     8641 gtcaacgaaa agaatattgc cgccttgaag aaaggaggta taatccgtcg attttttttg
     8701 tggctgcccc tcaaaggaga aagagtatga agatcgtgga agtcaaacac ccactcgtca
     8761 aacacaagct gggactgatg cgtgagcaag atatcagcac caagcgcttt cgcgaactcg
     8821 cttccgaagt gggtagcctg ctgacttacg aagcgaccgc cgacctcgaa acggaaaaag
     8881 taactatcga aggctggaac ggcccggtag aaatcgacca gatcaaaggt aagaaaatta
     8941 ccgttgtgcc aattctgcgt gccggtctgg ggatgatgga cggtgtgctg gaaaatgttc
     9001 cgagcgcgcg catcagcgtt gtcggtatgt accgtaatga agaaacgctg gagccggtac
     9061 cgtacttcca gaaactggtt tctaacatcg atgagcgtat ggcgctgatc gttgacccaa
     9121 tgctggcaac cggtggttcc gttatcgcga ccatcgacct gctgaaaaaa gcgggctgca
     9181 gcagcatcaa agttctggtg ctggtagctg cgccagaagg tatcgctgcg ctggaaaaag
     9241 cgcacccgga cgtcgaactg tataccgcat cgattgatca gggactgaac gagcacggat
     9301 acattattcc gggcctcggc gatgccggtg acaaaatctt tggtacgaaa taaagaataa
     9361 aaataattaa agccgacttt aagagtcggc ttttttttga gtaaagcgcc tataacacat
     9421 aatacagagg ataatactat gacgcgccgt gctatcgggg tgagtgaaag accgccactt
     9481 ttacagacaa tcccgcttag tttgcaacat ttgttcgcca tgtttggtgc aaccgtcctg
     9541 gtgcccgtct tatttcatat taacccggcg actgtactgt tatttaacgg tattggaacg
     9601 ctgctgtatc tcttcatctg taaagggaaa attccggctt atcttggttc cagctttgcc
     9661 tttatttcac cggtattgtt actgttgccg ttagggtatg aagtcgcgct gggcggcttt
     9721 attatgtgcg gcgtgctgtt ctgcctggtt tcttttatcg tgaagaaagc ggggaccggc
     9781 tggctggacg tgctgtttcc acctgcggca atgggcgcaa tcgttgccgt catcggtctg
     9841 gagctggcgg gcgtagctgc cggtatggcg ggtttactcc cggctgaagg gcaaacgcca
     9901 gactccaaaa ccatcatcat ctctattacc accctggcgg tcacggtttt aggttccgtg
     9961 ctgtttcgtg gtttcctggc aattatcccg attttaattg gcgtgctggt ggggtacgcg
    10021 ctctctttcg caatgggaat tgtcgatacc acgccgatta ttaatgctca ctggtttgcg
    10081 ctgccaaccc tctatacgcc gcgcttcgag tggtttgcca ttctgactat tctgccagcg
    10141 gcgttagtgg ttattgccga acacgtaggg cacctggtag taacggctaa tatcgtcaaa
    10201 aaagatctgc tgcgcgatcc aggtctgcac cgttcgatgt ttgctaatgg cttgtcgacc
    10261 gtgatttccg gcttctttgg ctctacgcca aataccactt acggagaaaa cattggcgtg
    10321 atggcgatca cccgtgttta cagtacctgg gttatcggcg gggcggcgat tttcgctatc
    10381 ctgctttcct gcgtcggtaa actggctgcc gctatccaga tgatcccatt gccggtgatg
    10441 ggcggcgttt cgctgctgct ttatggtgtc atcggtgctt ccggtattcg tgttttgatc
    10501 gaatcgaaag tggattacaa caaagcacag aacctgatcc tgacttccgt gattttgatc
    10561 atcggcgtca gtggcgcgaa ggtaaacatc ggcgcggcgg agttaaaagg tatggcgctg
    10621 gcgaccatcg tcggtatcgg cttaagtttg atcttcaaac tgatcagtgt gctgcgccct
    10681 gaagaagtgg tgctggacgc agaagacgcg gatataacag acaaataatg taccgaccgg
    10741 gcagtgttgc tgcccggttc aaacatcatg ggattctgtg gtaaacttct cgcgattttg
    10801 tgaaatcctg gttgaggtat ctctgaacac accggcacag ctctctttgc cactttatct
    10861 tcctgacgac gaaacctttg caagtttctg gccgggggat aactcctctt tactggctgc
    10921 gctgcaaaac gtgctgcgtc aggaacatag cggttacatc tatctctggg cacgcgaagg
    10981 cgcggggcgc agccatctgc tgcacgcggc ttgcgcggaa ttgtcgcagc gtggcgatgc
    11041 ggtgggctat gtcccgctgg ataaacgcac ctggtttgtt ccggaagtgc tcgacggtat
    11101 ggagcatttg tcgctggtct gtatcgacaa cattgagtgt attgcaggcg atgagttgtg
    11161 ggagatggcg attttcgatc tctacaatcg aattctggaa tcgggcaaaa cacgattgtt
    11221 gatcaccggc gatcgtccac cgcggcagtt gaatctggga ttaccggatc tcgcgtcgcg
    11281 actcgactgg gggcagatct acaaattgca gccactttct gatgaagata agttgcaggc
    11341 gctacagtta cgcgcgcgtt tgcgtggttt tgaactgccg gaagatgtgg ggcgtttctt
    11401 gctgaagcgg ctcgacagag aaatgcgcac gctatttatg acgttggatc agttggatcg
    11461 tgcgtcgatt accgcgcaac gtaagctgac cattccgttt gtgaaagaaa ttctgaagtt
    11521 gtagataagg tgtttattgt cggatgcggc gcgaacgcct tatccgaact acgcgaagtt
    11581 aatccatagt attcgtaggc cggataagac gtggcaagcg tcgcatccgg caatgtgctt
    11641 caactagcga tggagcacat cttaacccac gatttccagt acctgctccg gtggacgacc
    11701 aatccgcgct ttgccattcg cgaccacaat cgggcgctcc attagcttcg gattatccac
    11761 cattgcctga atcagcgcct cttcgctaag cgaactgtca gccaggttca gttctttata
    11821 aagatcctct ttctggcgca tcaattcacg ggcgctattc atccccagca ttttcagcaa
    11881 atcgcgcagc gttgccgcat cggcgggtgt ctcaaggtag agtaccactt ctggctccac
    11941 gccgttttct ttcagcaggt tcagcgtttc ccggctcttt gaacagcgtg ggttatggta
    12001 gatttttacc tgtttggtca ttgttcttcc tttaatgcga attacatctt ggtataaggc
    12061 ttaaagcgtt cctgcagctg gcgcaactgg tcgatgcgcg catcgtaacg cgcttgttgc
    12121 aggctgccta atttcacctg cgaactggcg ctactcaaca gcgaaatggc ctgatcgagt
    12181 cgtccggcga gcgcataacc ttctgctcgc gcagccagct cctgatcgcg gttatttagc
    12241 gcggcttccg cctgtgccag taaatcccag ccgttgctgt catctttatt attaaaggtg
    12301 tagcgattca gaatattggc cgcttcttgt ggttgaccgc cttgtagata agcgttcgcc
    12361 aggttgagct gcaacaccgg attggtgcgc aaatcacggg catttttcag gcgattgatt
    12421 gcctcattgg ctttgttttg cccaagatcg atatcggtag ccagatcgag ataccatgcg
    12481 ttgccaggtt ctgccgccag taacggttgc agcgttttac gcgcctcgtc gtatttattg
    12541 gcttccatcg cctgtaaagc acgaccatat tgcgccgctc gttgctgacg aacgtttcct
    12601 ttcgcccatt catccagcaa atcactggtg agctggttac gtccggaatt atacatcccc
    12661 agtgtgcgcg ctttcgccag atagaaatct tccgacgact gcaccaccat cggtcgcatc
    12721 tgattagcac ggttgcgggc atctgccaga cgactttccg gcaacgggtg agtcaataaa
    12781 atttccggcg ggcgcgagga gtaacgcgcc tgatcgagta atttttcgag gaaggttggc
    12841 atcgcctgcg gatcgaatcc cgagcgttgc agcacctgaa taccaatgcg gtccgcttcc
    12901 tgttcatttt gctgggtgaa actgatcatt ccctgacgtg ttcccgccag tgtaccggtc
    12961 agcgccgcca tccccgcctg cggactggcc atcgccagta aaatagaacc taacgcgccg
    13021 acccaggtca gcggcgcgct gcgttgttga tcttccatcg ctcgcgccag gtgacgttgg
    13081 gtgacgtggg agatttcgtg cgccataact gaagccagtt gactttcgtt atcggaataa
    13141 cggaacaggg cagagtgcag caccacgttg ccgccaaaga aagcaaaggc gttaatttcg
    13201 tcgttgttga tcagaaaaaa atgaaacggt gtcttaaccg aattggcatg cgaaaccaga
    13261 cgcatcccca gcgaattaat atattgcgtt aacagcgggt cattaattaa cggcgcgctg
    13321 ccgcgtagct ggcggacata atagtcgccc atctgcattt cctgaccaat ggaaagcgtg
    13381 cttcctgcgg aggttcccat atccggcaag gtgtctgcgc tgtcggcaaa cgccggggct
    13441 acctgaccaa tggtcatagc agcaatgagg gttgcaacca ggtttttttt caactgcctg
    13501 aacataacct ctatcctgta tttctgaaga tagtcatttg accgatactg ttaaatatcg
    13561 ttcccaccgc gccgcctttg agtgtaacgg cgaagattag cccactcaaa tgacttttca
    13621 gtttcctgaa tccgtcataa aaagcgaagt cttttttgtg acatttcgat acaattgcgg
    13681 atcgcaatcc cgctcttgag gttcagggaa ggtttttatg ctcgaaatgt tgatgcaatg
    13741 gtatcgccgc cgttttagcg acccggaagc gattgccttg ctggttattt tagttgccgg
    13801 atttggcatt atctttttct ttagtggcct gcttgctccg ttgctggtgg ctattgtgct
    13861 ggcctatttg ctggaatggc caaccgtgcg cctgcaatct attggctgct cccgccgctg
    13921 ggcgacgtcg attgtattgg tggtttttgt cggtatattg ctactgatgg cgttcgtggt
    13981 actgcctatc gcctggcaac agggcatcta cttaatccgc gatatgccgg ggatgctcaa
    14041 taagctttct gactttgccg ccacgttgcc gcgccgctat ccggcgttaa tggacgcggg
    14101 cattattgat gcaatggccg aaaatatgcg cagtcggatg ctgaccatgg gcgattcggt
    14161 ggtgaaaatt tccctcgcct cgctggtcgg tttgctgacc attgccgtct atctggtgct
    14221 ggtgccattg atggtcttct tcctgctgaa agacaaagag cagatgctga acgccgttcg
    14281 tcgggtgctg ccgcgtaacc gtgggctggc aggacaggtg tggaaggaga tgaatcaaca
    14341 aatcaccaac tatatccgcg gcaaagtgct ggagatgatc gtggtaggga tcgccacctg
    14401 gctggggttc ttgctcttcg ggctgaacta ttcgctgctg ctggcggtgc tggtcggctt
    14461 ctcggttctt attccgtaca ttggcgcatt tgtggtgacc attccggtgg ttggcgtggc
    14521 gctattccag tttggcgcag gcacggaatt ctggagctgc ttcgcggtgt atctgattat
    14581 tcaggcgctg gacggcaacc tgctagtacc ggtgttgttc tccgaagcgg ttaacctgca
    14641 tccgctggtg attattttat cggtggtgat cttcggtggt ttgtggggat tctggggcgt
    14701 attcttcgcc attccattgg cgacgctgat caaagccgtg atccacgcct ggcccgatgg
    14761 gcaaatcgcg caagaataat cttcagcccg acgaactgtt tcgccgggct gatttttcag
    14821 gattcctgac gtaaataaat agcccgataa cacatactca ccagcactgc accgccgata
    14881 atattaccca gcatcaccgg gagcagattg gcggtaataa aatggctgac cgtcagtgcc
    14941 ggaaaattgt ctgcgctact gtgcgccagt tgccagaagg aggtaggggc gaaatggcga
    15001 atggcaatgg cgaacggaat cacaaacaaa ttggcgatac agtgctcaaa gccactggcg
    15061 acaaacaggg tgatgggcaa aatcatggcg acgattttgt cgcataacga acgcccgcaa
    15121 taactcatcc acagcgccag gcaaaccatc agattgcaca taatgccgag gctgacagat
    15181 tcagtaaatg tatgatgcat tttgccctcg gcgcagtgta aaaccgcgac tccccacatc
    15241 gcgttttcac tcatcaccag cccggaaaac cagattaaca aactgaaaca ggcaatacct
    15301 gccagattac cgcaggccac cagaagtgcg ttaatcagcc aagttcgcca actaataacg
    15361 cccctacttt ttgccattac cgtcattacc gacgaggtga acaggttggt gccgcaaacc
    15421 gccagcaaaa taaagccaag tgtaaagcaa aggccgccca ccagatgggt taatgcctgt
    15481 gaagacgggg catcggcgat aactgaaagg taaaaagtaa atccgatcgc cataaacacg
    15541 ccagcggaca tcgccattaa aaagaccgac actttactgc gggcaatttt atgggcggca
    15601 atccgttcag cgattgccgc ttttctggcg ctcaactgca agtcgaaaga gagtttgttt
    15661 ctcatgcata ctcctgcaaa agcagattac aacacctcgc gaaccgagat tcccagacgc
    15721 tgcattcgtg acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc gccacggggc
    15781 ccggcaacaa tgccattggt ttctcgcaat acctgaacaa tgcgctgacg ttcttcttcg
    15841 tcattctctg gcgtcgtcgg gtgcagcaac tgcgccattg aactgcgaag cgctgaatct
    15901 tcatttagcg tcggcagtaa acggctttgt cggacattaa gatgtaaatt cagactgtta
    15961 ccgcgagtca gcagtaccgc tcgctcaatc acgttttcca gctcgcgcac gttgcccggc
    16021 cagtcccacg acatcaactg gcgtagcgcc tcggtcggga tggcgtcaat tgaacgattc
    16081 atatggcgcg ccattttttg cgtgaaatgc tttgctaaaa gagggatatc ttccggacgg
    16141 tcgcgcagcg gcggcaattc cagtgggaag acattcaggc gataaaagag atcgctgcga
    16201 aactggcgat cttcaaccat ttgccacaaa tcacggttgg tggcggcaat gactctgaca
    16261 tttaccggga tcgttctact cccgccgaga cgctcaatct cccgttcctg caatacgcgc
    16321 agcagtttag gctgaagttc taacggcaga tcgccaattt catcgagaaa caacgtgccg
    16381 ccatcggcaa tttcaaaacg accacgatgg gtattaatcg caccagtaaa cgcccctttg
    16441 tcatgaccga ataactcact ttccagaaga ctggcgggga tggcagcgca gttgattttg
    16501 accagcggct tgtcgcgtcg cgggctaagt tgatggatcg ctctggcgat cacctctttg
    16561 ccggttccgg tttcaccgca aatcaacacc gtactgtcgc tcttcgccac aatatctacc
    16621 tgctggagta ggtcttccat tgcctggctt tgatagataa tgtcaccgat gcccagattc
    16681 gaaaggagct gctcgctaag ctggtggttt tcttgctgca aactttcctg caaatcggtc
    16741 atgctacgcc aggcatcggc attgccaacg gcaatagcga tgcgatcggc tatgtgttgt
    16801 agtagctggc agttttcctc actaaagaga gtggaagagg tatgcgccag caacaatgca
    16861 cccggtgtat ggttgccaaa ggtaagcggt ataaggagcg cagattcgca gccgttagat
    16921 gcaagtaaga gtaataacgg gtcgcgttgc cagagaaaca gatcgtctgc acggtgcgtc
    16981 aaggtcggtt gtcgggtttg tagcgtttgt gtcagcaata cactgtcgcc aggcatattg
    17041 cgtggcagac acgcacaatg tgaggcagaa agatcgctgc accacaggct aaacttctcg
    17101 ttctttcgat gatcgcccag taccatactg actgaagcca gaccgaaaaa atgatggatc
    17161 tcacgagcga cgtcagcgat cagatcgtcg agatcaagat gtgacagcac tgcattagta
    17221 atatcaacca gtacccgatg atgatcgcga tctttctcca gcaaacgcag cgccacctgc
    17281 tgttcttcat cattgagcca tgattgcaga accggggtga ttatccgcgc cagccagtta
    17341 aacagtgtgc aatcctgggg agtaaaacgg ctgccgtggg tacgaataaa gcgtagctcc
    17401 gccattccac tactgccgag aaggacggcg tgacgcgaat aatcgaccat ctcgccctga
    17461 tactctgttg ttgccgtcac atcgctgcca tcatcatggt agcggtaata gaagttgctt
    17521 ttgagaacat gcagttcaat aagttgtacc gggctgaacg gcggcggcat cgggatgaag
    17581 gcgcgtaata aagacgcctt accgtgtttc tgtgctaacc gctccgcgag cattccgttt
    17641 accgcttcaa ttgttattcc ttgtggcggg gcaaacatcg cctcgtctga catagccatc
    17701 aacagtcctt attaatgaga aatccttctc acgcaatttt tctggccatc agatagatgg
    17761 ccggttcctg cacaatttca tccaacagat gaatgaattg aatgctacgc gcctgccaca
    17821 cagacgtcgc gtcatccgcc agtggcgcga atgcccgggt caacagggcg gtgtggctgg
    17881 cgtcaataac aatctcacca aaagtcatca cccccagcat cttacgtcgg gcttgctcct
    17941 cttcgacctg ggcaagccaa tcttcgtatt ccgtcagtgg gcaggggaag gcgacattca
    18001 gacaatcaat cacgccaacg tgatgaccga tagccagcga gtaatacatt acctgagagc
    18061 tgtgttccgg catctcgtcg ctactggcga caaacttttt tcgcagcgtc cagaaaacaa
    18121 tttccccgca ctcttcagtc atgtaacccc cggagtacgc gaaagagttg ctgaacgata
    18181 ctattgagac gtggatcgtc ggcatcgcgc aaccaggtgt taacccgatt tcctgtagga
    18241 tcgtcgctta aatggcgcag gagccgatcg caaatttctc gcccctgacg atagcccgcc
    18301 agccgccgcg cttctcgctc aatggcgata cgctgtgatg gcgggatctg cggccacaac
    18361 ggttgcatag tcaccccggt gggatcgcga taatccaccg cgtgaatctt ctgttgcagc
    18421 aaaccgagcg ccacggcgaa accgtgaatg gtggcggccg gtgttggcgg gcagccgggg
    18481 atccaaacat caatggggac aatggtgtcg ctgccgcccc agacgctgta gagatcgtgg
    18541 aaaatccgcc gccggcaccg cacgcgccgt aggaaacaca gattttatga tcgggggcag
    18601 actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct gtaaataaca
    18661 aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct gcgtcgaata
    18721 ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg caatcgacgc
    18781 gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc ttttgcgttt
    18841 gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc atagctgctc
    18901 cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt gtttgcactc
    18961 cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat tttgttgctg
    19021 tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc gttcgcaacg
    19081 gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat tggtgacggt
    19141 cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt cacaacgtcc
    19201 gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct ggtcgtcggt
    19261 ctggatagtc agcgcatttg ccggacaagc acaggcgcag gcaccgcagg caatacattg
    19321 gctgggcatc aggtccggtt tcccgcgaaa gcctgggctg acctccagtg gcgcgaaggg
    19381 atatttcacc gtcgcggttc cggcgcgcat aatagttttc agtaacttca gcatcttctg
    19441 tccttacttc agcggcgaac ggttacgatc aataccgtag cgttcgatct ctttatacgg
    19501 cacggttttt gactggcgct tgcgcacatc taccagcgtc acacggtcgg tacaggagta
    19561 gcagggatca aggctaccga taatcagcgg tgcgtcagaa acggtattgc cgcgcagcat
    19621 gtaacgcaac accggccagt tggcgtaggt ggcggcacgg cagcgccagc ggaacaattt
    19681 ttggttatca ccgagcatgc tccagtgcac gtcttcaccg cgtggcgctt caacaaagcc
    19741 cagcgcgaat gcgtgaggtt tatagctaaa gccttcggtc agcagtgggg tatccggcat
    19801 gttgtcgagg gcaaattcca gcattgccag cgaatcaaac gtctctttga cacggaccat
    19861 cacgcgggag aaaacatcgc cgccggtaaa ggtaaacagt gtttttggaa tattgccgta
    19921 gtcggcgtag gggtgatcaa agcgcaaatc acgggcgatt tgtcggtcga gaatgccaat
    19981 gccctgagtg cgttgttcca tattcggcgt agcaagcagc atctctacca gctccgacac
    20041 gtcggcgcgc atctcgcgca ccagtttcag cgtttgcaga cgttgctctt tgagaatatc
    20101 gcggcgaaca ccaccaatca gattcagacc gtaggtttta cgcgacccga tcagcaattc
    20161 cgccatcgtc atcgactttt cccgcacgcg gaaaaattgc ataaagccgg tatcaaaacc
    20221 aacgaaatgg caggagaggc caaggttaag caaatgactg tgtagccgtt cgacttccag
    20281 cagaatcgag cgaatagtat gtgctcgttg cggcacctca atccccagtg cattttcaac
    20341 cgagttggta taggccacac tgtgggcaaa accgcaaatc ccacacacgc ggtccgataa
    20401 gaaggtcact tcgttgtagc ccatccgcgt ttctgccagt ttctccatgc cgcgatggac
    20461 ataaaacagg cggtaatcag catcgacaat ttgctcgcca tccacaaaca agcggaagtg
    20521 acccagttca tcggaggtga tatgcagcgg gccgacaggg atcacctgcg catcgctgtt
    20581 gccctcattg ataaacggat acgtttcgga atcagtcgtc ggttcagggc gcagtcgata
    20641 atccatcgca tctttgcgca gcggatgcat atcttccggc cagtcatcgg gcaacaccag
    20701 gcgacgatga tccggcaggc caaccggaat cagcccgtac atatcgcgaa tttctcgctc
    20761 gccccagacc gcggcaggga cgcgcggtgt gactgacgga aactcccgac tgtcggcatc
    20821 gaccagtgcc ttcaccacga tccagcattt ttcggcccct tccattgaaa gggcataata
    20881 aaccgcgtaa tgaccgttaa gtgtccgctc gtcgttgcca aacaggactg gaagccagcc
    20941 atcatgttga taataaagat aatgtacaac gtcaggcagc agattgattt tcaccgtaat
    21001 ggtgacctgt tcaggcgttt gtcgctcttc atccagcacc gcgccgggga actgcgtttt
    21061 cagggcggcg agaatcgctt cgccacgatt tgacgatgaa ttaacgttca ctgtgtttct
    21121 ccgaaacaga tgcggtgcct gaaggcaaaa aatcatgcca ggtgctacgt tgtgcaggca
    21181 ggtcgtgcgt ccctgagagg actatagtgg aagcgcccgc caggatcctg atgacaggtt
    21241 gtggaatatg cgttcccatc gccagcatca tgaccagcag aatcaccatt ggcgaggtgg
    21301 tcaaccagcc gagatcaccc cggttaacgg cctgcggcgg tttcgccatt aacacccgcg
    21361 ctgccatccg taccaggccc gccagcacca gcgttaacag caataacagc aggacgataa
    21421 tcagcaggtg attacgtgcc agtccggcgg taatggtcat aaattcgcta agaaaaatgt
    21481 tgaagggcgg catccctgcc agcgccagcg caccgccgcc aaacagcacg gcggtaaatg
    21541 gcatgatttt gagcatccca cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta
    21601 cattgccgga accgcagaac agcagcgttt ttgccagact gtggtttaag gtgtgcagca
    21661 gcgcggcaaa aattcccagt ggcccgccaa tgcctagcgc caccgcgacc agtcccatgt
    21721 tctccacgct ggagtacgcc agcagacgct taatgtcccg ctgtaccaga atgaaaaatg
    21781 ccgccacggc aaccgacaac atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc
    21841 cgatggcctg gcagataatg atgtaatagc gaattagcac caacagcgcg cagttcagca
    21901 atacggcgga gagcagggcg ctgaccggac tcggtgcttc actgtgagca tccggcagcc
    21961 aggcgtgcat gggaaatagc ccggttttgg tgccaaagcc aattagcaca aacacaaagg
    22021 ccaacagcat taatgttggg tcaagcaagg acgattgctt aagaacctcg ctccagaata
    22081 tcgccatttc tgcctgcggc ataacgctgg cgacgttggc gtataccagc acggtaccga
    22141 acagaccaaa agcgacacca acagtacaaa taatgatgta cttccatgct gcttccagcg
    22201 atgaacgctg accgtaaatg cctaccagaa acgccgagct taaggtggtg gcttcgatcg
    22261 ccgcccacat cacaatcagg ttattgctgg taacaaccag cagcatggta aacaaaaaca
    22321 gatggaagaa accgtagtaa tcgcacagcg taacggggga aagctcgccg tgtgccactt
    22381 catgacgcat gtagccaatc gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg
    22441 cgaggaacaa accgcccaga ccatcaatat gaagccacag tcccgcagcg aatatttctc
    22501 ctgcatcagc ggcagtttgg accacccaga gtgccagaat cagcagcagt gtgatcccta
    22561 agctatgtaa tacagtcacc gtacaagtcg cagagagccc ccgtttccgg caggcaaaac
    22621 agagcagcga aaaaagcagc ggcgtgagca ggagtaaagc gaacatcaca gaataactca
    22681 tctcattatc ccttcagcgc ggtcaagttg ttcacgtcca gcgtgccgtg ggtacgccat
    22741 atttttcttg ccagtaacac catcacaatg acggcgaaga tggcgtcggt agcgatacct
    22801 atttccacca gttccggtgc tcgccaggca agaagcgcca gcaccagatg ggagccgttt
    22861 tccatcaggc agtaaccaaa aatttgccgc aggatattgc gctggctgac aatgcacagc
    22921 aggccaagca gaaaatgacc taacgctacc gccagcgccg gtttcagccc ggtagccatc
    22981 ggtagcttca cgggctgaac gacaaatgcg caaagcagga caattaacgc cgcgagcagt
    23041 gccatcattg ccggaccgaa taacgctttt tccgggatgt tctggggaat atttcgtgca
    23101 gcgtaagtca tgattaacgg taccagcagc actttggtga taaaggcgct ggcggaccag
    23161 atcagcagtt gctctgcggc gaacaggcac gagagagtgg cgaaaataga caccagcacc
    23221 agcgactggc aggcgtaaaa tccgcatgac aggcgatagc ttttgacgct aatcacaaac
    23281 agcgatgtca gcatcatcag tcccgccaga ttatttacga tcatagaacc ggtcattcat
    23341 catctcctta ctgcaaccag gtcgcggcga ttacgctgga aatcagcgac atcacaatca
    23401 gtacaatcaa caccaggcgc attgaccctg gcagcggtgc ggcatcggcg acggcctcgc
    23461 tcggtttgcc aggcacaacg cgaccaaacc agcgaataaa ccaggcgaaa ctggcgaccg
    23521 attcaatcat cagcagaatc atggcgggca gcaggatcca gtactccact gacaacgcaa
    23581 aaccggcagc aaacagcggg aatttactga agaagccgtt gaacggcggc acgccggtaa
    23641 tcgccagcgc tgccacgcag aaacccacgc ctggcaacgg cagggtgtgc aatacgccac
    23701 gcagacgcgg caacaagcgc gtgccgcagc tgtaactcag cgcacctgct acaaggaaaa
    23761 acaggctttt agcgaacgcg tggttgacga tgtaggcgat gctgccctcc agcgccagcc
    23821 gcgagccgaa gatggagagc gacaaaccga agaacatcca gccaagttga gtgatggtcg
    23881 accaggccag caaccgcttc atatcctgct gtggcaaata catcagaaag ccataaagaa
    23941 tggtgaccag cgccatgacc atgccaacgc cgccaatcac atgcgggata ttgccgccgt
    24001 cgataatggc gcgggcaaaa atgtaaacgc ccactttcac cttcgatgcg gcgtggagat
    24061 aggcactgat cggtgttggc gcttccattg catccggtag ccacgcttgc atcggtagct
    24121 gggccgattt cccccacgcg gcaaacagga tgccgccata aaccagataa cgtgcgtcgc
    24181 cgtgtaactc gctcatcgcg ctaagcgcaa acgttccggt ctgcaaaaac agcgtggcgg
    24241 cggcaagata caaccccaac gagccgatat gagtgataag taacgctttt agtgctgaac
    24301 gctgtgcttt atcgctctgg taataactga tcaacgccca ggagcagccg cccgtaattt
    24361 caaaaaacaa caactgaccg agcagcgtcg aggagagtac cagtcccgcc atcgcgccga
    24421 taaacaacag caaaaatgcg taataacgat tcgtgccgtt atgcgggtgt tcgcgatttt
    24481 tatccgtcag ataacccgtg gagtagatcg tgaccagcaa accgagaaac accaccacaa
    24541 acagaatcag cgtactcacg cgatcaatga ccaggccaaa caatgccaca tcccccacgt
    24601 tgaccaatgt caacgtgacg gcaactttat cggcctgata aaacgcggag atcagtgaca
    24661 acatgcacag cgtggtcagc gcggcgaaca aaacccccca ttcggcggcc cgacgttgtg
    24721 gcgaaaacga aacgaccagt gcgccgataa aaggcagcaa taacgtcgtc agagcaagat
    24781 tttccatatt ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca
    24841 gcaaggctga agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc
    24901 gtgttttctg cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca
    24961 agtgaagtca gcaggcaggc gagagaaagt tcttgcgcgc gcccaaagga cagaaacagg
    25021 gccacaaaca gtgatgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc
    25081 agcccggcac cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca
    25141 acatcaaagg gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc
    25201 agtacggtgg ttagcggcga gttccagccc atcgccagcg tattgctgat catctcgata
    25261 tgcgtggaac ctgctatcag cgccaatacc agcagtgaga gaataagcat tggttcgacc
    25321 agaatgccga gcgtcaactc gcgactggca ccgactcccg caaacggact tccggtatcc
    25381 agcccggaaa gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga
    25441 tcgccgccgc ccgcaaaagg ggaaacggta ataaacagtg gtaaggccat cgccagcacc
    25501 agcatgctgc tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc
    25561 gcaacttcct ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc
    25621 ccgcggcggg agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt
    25681 agcatcagca gaatcacggc ctgtgctaac gcaaaaataa ccagatatcc gtcgcaaaga
    25741 gtttgtctca tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc
    25801 gaccacatac aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc
    25861 cagccgttgg gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt
    25921 gctctgagcc ctggcggtga cgtgcgcaaa acctttattt agcctcagcg tagggtcgag
    25981 ctgttgtcgt agacgaaata acgcagagaa gaccacacgc agcggctgca tcacgccatt
    26041 gcctgacggg gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg
    26101 acgaaacgct gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat
    26161 cgccagtagt aacagcaaca gtaatgaggg ggtgacctgt gtatgaaacg tgccgggtac
    26221 aagtgctatt ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatatg
    26281 cattatcttc ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag
    26341 cagtagcatg gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtact
    26401 gcgcggcgca ccacagaaag taataccgta catttttacg aagcacatta ctgccagccc
    26461 accagtgact gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc
    26521 cacacgactt agtgagaaca gcgactgcca ggtgtaccat tcgctgataa aaccattcag
    26581 cggaggaatg gctgatatcg cgaggcaacc aatcaggcat gctgcggctg tccacggcat
    26641 ccgtttcgcc agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc
    26701 gcccgcgccg agaaatagca gccctttgaa cagcgcatgg ttgagcagat gaaacagtgc
    26761 gccgagcagt ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac
    26821 accgactgcc agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt
    26881 gatatcctgt tccgccagcg catacagcac gcccaggagc gcggagattg cgccgatcgc
    26941 catcaccaga atgccccacc acagcggcaa acccgtttgc gccagcaggt ccatcgctac
    27001 tttcaggatg ccgaaaatac cgattttgac cattacgcca gacatcaacg ccgaagcgtg
    27061 cgatggtgcg gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt
    27121 cgcgccaaaa ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga
    27181 aagcgtgcgg aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc
    27241 gatcataatc agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat
    27301 cgacgtttta tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa
    27361 cagcacgatg aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa
    27421 aagattcata aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt
    27481 caatgaatag agcgaacaaa ccaccaccag caaggagata accagcacca taaatgccga
    27541 gaggctatcc attcgcaggg taaaatcggc aaagtcaaaa ggggtggcaa aacgggcgac
    27601 taaagttaca ccagcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac
    27661 cagcgatgtg atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga
    27721 agccagactg gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat
    27781 tagcgttgct cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg
    27841 gcaaggcgct gtttttcttt catctgtcgt tgcaggctat cgtcggtgat cagccgtaac
    27901 gcctgattcg ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg
    27961 catttcaccg cgacggtctg gacacccggt tcccagcgca gcaatgcatg ttggggcggc
    28021 acgttttctt cgccgtcttt gagtgagcct tcagcctgaa aaacatattg cgcaggggca
    28081 ttcaccggac ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc
    28141 ttgcagccaa tacagaggct ttcgttgagt tggatcgcat catccctctg ggagatggca
    28201 ttaaccgggc agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca
    28261 gtgagtgttg acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta
    28321 tgcacgtccg aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc
    28381 accacaaagc ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg
    28441 cataaaggat gccattctga ttaactcaat atattcaata agttatgtat tgactggatt
    28501 ctgaacggta caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg
    28561 tcgcagggga aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag
    28621 cgcagccagc acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca
    28681 acgtcgtggt gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg
    28741 tcaatcagga agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca
    28801 ccccagacgc cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta
    28861 agcagctctt tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc
    28921 acatcaacgc ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg
    28981 gtacagccgg gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag
    29041 tcggtcaaat taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca
    29101 ccggctttca gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga
    29161 cgacgttaat actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa
    29221 tatttgctgc gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag
    29281 cccgttcatt ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg
    29341 cgtcgaaaag tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa
    29401 cccagacaga tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta
    29461 aaagatccag ttcggcacct ttcaacggta acgttgattc aatcagagtg atggcattcc
    29521 atgaaccgga aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt
    29581 cttcaatatt acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc
    29641 gatcggcacc caacgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta
    29701 ttcctttaag gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc
    29761 agaacggttc tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt
    29821 tgcacagagg atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg
    29881 aaaaaggtaa tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg
    29941 gtacttcggc gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg
    30001 aacatgctga tgtggtgatg atgacgctgg atctggctga tgggcgcatt ccggtaattg
    30061 ccgggaccgg cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca
    30121 gtggtatcgt cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt
    30181 tgtatcagca tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg
    30241 tgccgtcccg tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa
    30301 aaaatattat cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag
    30361 agctggtttc agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca
    30421 tgcaattggg cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg
    30481 cccagatgtg caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc
    30541 gtctgatgcc attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg
    30601 catgtaagga actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca
    30661 ccgacagtgg tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt
    30721 tagggagatt tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt
    30781 tcgcttgttt tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt
    30841 ggtgatgaag cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg
    30901 attttgccgg tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc
    30961 ggtaaggcgc tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt
    31021 acccagttca cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg
    31081 tggccgcagg tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat
    31141 gctggtcaga cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag
    31201 tatcgtggtc gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt
    31261 aaactgctga acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt
    31321 tacagcacgg agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc
    31381 gcgaacgctg cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac
    31441 accggtttac caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca
    31501 gcggcgctgg aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg
    31561 gccgtaactt ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca
    31621 ggcctggcat ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta
    31681 cagttcatcg atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct
    31741 gtcttccagg ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt
    31801 ctgatatgat acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca
    31861 cgaaatttgt ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat
    31921 ccggcacacc agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga
    31981 tgcaaaagca agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc
    32041 cggacctgtt ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg
    32101 agcagtttga tcgcaaaggt atggtgaaca acaagttcaa ttacttcatt atgagcaaac
    32161 tggctgaagc gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg
    32221 tgaaaaagct ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gctggctctc
    32281 tggtgaaacg tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt
    32341 tcctgaaaaa cgacgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg
    32401 gctgggtgag caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg
    32461 tgctgaaaaa actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg
    32521 gcctgtacaa aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt
    32581 gggacaaaga aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc
    32641 tgatcgaagc ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct
    32701 ggttgcttgt ttctttagcc ggagggtact ccggctaaat gtctttgata cccccgctac
    32761 aatacttctc attatctccc tttgacagga aagtcatacc aggccaatat gtatatacat
    32821 aacgtatatc ccaaaaaggg ggggctatgg aaactactgt atttttaagt aatcgcagtc
    32881 aggccgtcag attaccgaaa gcggtcgctc tgccagaaga cgttaagaaa gtagaaatta
    32941 ttgcgattgg tcgtacaaga atcatcactc ccgccggtga gtcctgggac agttggtttg
    33001 atggcgaaaa tgtgagtgcg gattttatgg atatccgtga tcaacccgca atgcaggaaa
    33061 gggaatcatt ttgatgctga aattcatgct ggataccaat atctgtattt tcactattaa
    33121 gaataaacca gtacatgtca gagagcggtt taacctgaat tctggccgga tgtgtataag
    33181 ttctgtaaca ttgatggagc ttatctatgg ggcagaaaaa agtcagatgc cagaacgcaa
    33241 tttggcggtt attgaaggtt tcgtatctcg tttggtagtg cttgattatg atagtgctgc
    33301 tgcgacgcat accgggcaaa tcagggccga gcttgcccgg cagggaaggc ctgttggacc
    33361 ttttgaccag atgatagcgg gacatgctcg cagtcgcggt ttgattgtgg ttaccaacaa
    33421 tacccgagag tttgagcgag tggcgggaat cagactagaa gactggagct aacattctct
    33481 tttacttcta acattcgtaa attatataac gtggcatgaa gccttaattc ctccgccatt
    33541 tcatcccgct ttcctgtggt aatcttcctc tgaaccacct acgatcattt ctataatgaa
    33601 tatattgttg aggtatctat gcgttggcaa gggcgacgtg aaagtgacaa tgttgaagac
    33661 aggcgcaaca gctccggtgg tccgtctatg ggcggtcccg gttttcgcct gccaagcggt
    33721 aaaggcgggt tgattttact gttagtcgtg ctggttgcag gctactatgg tgttgattta
    33781 accgggttaa tgactggaca gccggtttcc caacaacaat caacgcggtc cattagccct
    33841 aatgaagacg aagctgcaaa attcacctcg gtgattctgg caaccacgga agacacctgg
    33901 ggacaacagt tcgagaagat gggtaagacc tatcagcaac cgaagctggt catgtaccgt
    33961 ggaatgacgc gcaccggctg cggggctggt cagtccatta tggggccgtt ctattgcccg
    34021 gcggatggca cagtttatat cgatctctcc ttctatgatg acatgaaaga caaacttggc
    34081 gcggatggcg attttgccca ggggtacgtt atcgcccatg aagtcggtca tcatgtgcag
    34141 aaactgttag gcatcgagcc gaaagttcgt caactgcaac aaaacgcgac gcaggcggaa
    34201 gtgaaccgct tatctgtgcg tatggaactc caggccgact gttttgccgg tgtctggggg
    34261 catagtatgc agcagcaagg cgttctggaa accggcgatc tggaagaggc gctgaacgcg
    34321 gcgcaggcca tcggcgatga ccgtttacaa cagcaaagtc aggggcgagt agtaccagat
    34381 agtttcacac atggcacttc tcagcaacgc tacagctggt ttaaacgtgg tttcgacagc
    34441 ggcgatccgg cacaatgcaa tacttttggt aaaagcattt aacttccggg gcagggatgg
    34501 ctgaactgac tgcgcttcac acattaacag cgcaaatgaa acgtgaaggg atccgccgct
    34561 tgctggtgtt gagcggggaa gagggttggt gttttgatca tgcgcttaag ttgcgtgatg
    34621 ccttacctgg cgactggctg tggatttcgc cgcagccaga tgctgaaaac cactgttcac
    34681 cctcggcgct acaaacttta cttgggcgcg agttccggca tgcggtattc gacgcccgcc
    34741 acggctttga tgccgctgcc tttgcagcac ttagcggaac gttgaaagcg ggaagctggc
    34801 tggttttgtt actccctgta tgggaagagt gggaaaacca acctgatgcc gactcgctgc
    34861 gctggagtga ttgccctgac cctattgcga cgccgcattt tgtccagcat ctcaaacgcg
    34921 tacttacggc ggataacgac gctatcctct ggcggcaaaa ccagccgttc tcgttggcgc
    34981 attttactcc ccgtactgac tggcaccccg cgaccggcgc accacaacca gaacaacagc
    35041 aactcttaca gcagctactg accatgcctc cgggcgtggc agcggtaacg gctgcgcgtg
    35101 ggcgcggtaa gtcggcgttg gcagggcaac tcatttctcg tattgcgggc agtgcgattg
    35161 tcaccgcgcc cgcaaaagcg gcaacggatg tactggcaca atttgcgggc gagaagtttc
    35221 gctttattgc accggatgcc ttgttagcca gcgatgagca agccgactgg ctggtggtcg
    35281 acgaagccgc agccatacct gcgccgttgt tgcatcaact ggtatcgcgt tttcctcgaa
    35341 cgttgttaac cactacggtg cagggctacg aaggcaccgg acgtggtttt ttgctgaaat
    35401 tttgcgctcg ctttccgcat ttacaccgtt ttgaactgca acagccgatc cgctgggcac
    35461 agggatgccc gctggaaaaa atggttagtg aggcactggt ttttgacgat gaaaacttca
    35521 cccatacacc acaaggcaat atcgtcattt ccgcatttga acaaacgtta tggcgaagcg
    35581 agccagaaac gccgttaaag gtttatcagt tattgtctgg tgcgcactac cggacttcgc
    35641 cgctggattt acgccggatg atggatgcac cagggcaaca ttttttacag gcggctggcg
    35701 aaaacgagat tgccggagcg ctgtggctgg tggatgaggg gggattatct caacaactca
    35761 gtcaggcggt atgggcaggt tttcgtcgcc cgcggggtaa tctggtggcc cagtcgctgg
    35821 cggcgcacgg tagcaatcca ctggcagcga cattgcgtgg acggcgggtc agccggatag
    35881 cagttcatcc ggcgcgtcag cgggaaggca cagggcagca acttattgct ggtgctttgc
    35941 aatatacgcg tgacctcgac tatctttcgg tgagttttgg ttacaccggg gagttatggc
    36001 gtttctggca ccgctgcggt tttgtgctgg tgcggatggg taatcatcgt gaagccagca
    36061 gcggttgcta tacggcgatg gcgctgttac cgatgagtaa tgcgggtaaa caactggctg
    36121 aacgtgagca ttaccgttta cgtcgcgatg cgcaagctct cgcaaagtgg aatggcgaaa
    36181 cgcttcctgt tgatccacta aacgatgccg tcctttctga cgacgactgg cttgaactgg
    36241 ccggttttgc tttcgctcat cgtccgctat taacatcgtt aggttgctta ttgcgtctgc
    36301 tacaaaccag tgaactggca ttaccggcgc tgcgtgggcg tttacagaaa aacgtcagcg
    36361 acgcgcagtt atgtaccaca cttaaacttt caggccgcaa gatgttactg gtccgtcagc
    36421 gggaagaggc cgcgcaggcg ctgttcgcac ttaatgatgt tcgcactgag cgtctgcgcg
    36481 atcgcataac gcaatggcaa ttttttcact gactccttca attttctccc atggtcattg
    36541 tgagtcagcg gcgtaaaatt aatgacatca gttaaggagt tcgccatgaa acatgaccat
    36601 tttgttgttc aaagcccgga taaacctgcg caacagttgt tactgctttt ccacggtgtc
    36661 ggggataacc cggtggcaat gggggaaata ggtagctggt ttgcaccgct gtttcctgat
    36721 gcgctggtgg tgagtgtcgg tggcgcggag ccgagcggta atccggcggg gcgtcagtgg
    36781 ttttcagtgc agggtattac ggaagataat cgccaggcgc gtgtggatgc catcatgccg
    36841 acgtttattg aaacggtacg ctactggcag aaacagagcg gagtaggggc taacgccacg
    36901 gcgctaattg gtttctcaca aggcgcgatt atggcgctgg agagcattaa agccgaaccg
    36961 ggccttgctt cgcgcgtcat tgcctttaac ggacgctatg cgagcctgcc ggagaccgcg
    37021 tctacagcca cgacaattca cttgattcat ggtggtgaag atccggttat cgatctggcg
    37081 catgcagttg cggcgcagga agcgttaatc agtgccggtg gtgacgtgac gctggatatc
    37141 gtggaggatt taggccatgc gatcgacaat cgcagtatgc aatttgccct ggatcacttg
    37201 cgttatacca ttccgaaaca ttatttcgac gaagcgctaa gtggcggtaa gcctggcgat
    37261 gatgatgtga ttgaaatgat gtaaaagaca gattacttgg cctgataaga cgcgtgagcg
    37321 tcgcatcagg catcgttcac cattgccgga tgcggcgtta acgccttatc cggcctacgg
    37381 ttgagcactc agcttacttc ttcggttgat cctttttcgg ccagtcgtcg tcatcgtccc
    37441 acttatcgtt gaaatcacga tgcgggggaa gctccggctt gttggcgaga aatttcttat
    37501 ggtcgacgcg cttgagatct ttaatcacgt tcagcaggac gcctaccaaa aagacaatca
    37561 ccagaatcca ccaatattta gccagccagt ccatgcttat ttcctcttgc agaaccactc
    37621 atcaggcgac gagctgttcc atgatacgtt gatacatacg ggcaagtagc tgcaggttgg
    37681 cagcgttcac acattcatta attttatgaa tagtggcatt gaccggcccg agttccacca
    37741 cctgcgcccc catgcgagca ataaagcgcc cgtcggacgt tccgcctgtg gtcagtagct
    37801 gcggtttaat ttcattatag tgctcaaccg cgttaacgac cgcatccacc agtttaccgc
    37861 gcgcggtcaa aaatggctgc ccggaaagcc accagtccac cgtataacgc agttgatgtt
    37921 tttcaagcag ggcaagcacc tgtgctttga tcatctcatc agtcagttcg gtgctgaagc
    37981 ggaagttaaa ctgcacaaac agttcacccg gaataacgtt gttactgccc gttcccgcct
    38041 gaatattggc aatctgcata ctggtcgccg ggaagaattc attgccctga tcccactcaa
    38101 tagccactaa ttcattaagg aaaggtgctg cgcgatgtac cggattgtca gccagatgcg
    38161 ggtaggcaac atgcccctga acgccatgaa tggtgaggtt gcaggttaac gatccgcgac
    38221 gaccattttt caccacatca cctaccactt cgatactcga cggttcgccg accaggcagt
    38281 aatcgagacg ctcattacgt gccattaacg cttcgacgac ttttaccgta ccgttgtgtg
    38341 cactggcttc ttcatcagag gtgatcagaa atgccagtcg ccccgtatgg ttgggatgtt
    38401 gtgcgacaaa gcgttctgcc gctaccacca tcgccgccag cgagcctttc atatctgccg
    38461 caccgcgccc gaataacatg ccatcacgaa tggtgggttc aaacggcgga ttgatccaac
    38521 gatcggcgtc gccaggcggc accacgtcgg tatgcccggc aaaggctaac gtttcacctt
    38581 gcccacgcca tgcccaaaaa ttctgcgtat cggcaaagtc catgcgttca acggtaaaac
    38641 cgatcgcctg caaacgttca atcaacaaag cctggcatcc tgcgtcatca ggactcaggg
    38701 aagggcggcg aataagctgt tgtgtcagct caataaccgg gcacgacata gactacacct
    38761 catggaaaaa ttgctgataa ctggaatcac tgaaacccag cagcataggc ttaccaggcg
    38821 cgcagagcaa tggacgtttg ataattgcag gcatttcagt cattaatgcc gccgcagagg
    38881 ccgcatcggt gattttattg cgggtggttt cgtccagttt acgccaggtt gtaccacggg
    38941 tgttgagtaa cgcttcccag cctaattcgt tgataaaacc gttcaataat tcgctgtcca
    39001 gcccatcgac gcggtaatca tgaaaacgat agtcgatgtt attggcttcc agccaacggc
    39061 gagccttttt aatggtgtca caatttttga tgccgtaaag tgtaaccata atgaatcctt
    39121 tttcgattta tgagaataaa tactcattta agggtgaaaa cattttgcct gcaaatgatt
    39181 atcagaaaat ataa
//
LOCUS       DAZFJF010000032        38582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFJF010000032 DAZFJF010000000
VERSION     DAZFJF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.032"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..267
                     /locus_tag="ACHKAU_001837"
     CDS             <1..267
                     /locus_tag="ACHKAU_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO5530916.1"
                     /translation="QRLAERQRKTKPLLKSLESWLREKMKTLFFGSGHGGERGALLYS
                     LIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
     gene            306..659
                     /locus_tag="ACHKAU_001838"
                     /pseudo
     CDS             306..659
                     /locus_tag="ACHKAU_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
     gene            complement(699..1169)
                     /gene="yehS"
                     /locus_tag="ACHKAU_001839"
     CDS             complement(699..1169)
                     /gene="yehS"
                     /locus_tag="ACHKAU_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO5530917.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(1216..1935)
                     /gene="btsR"
                     /locus_tag="ACHKAU_001840"
                     /gene_synonym="yehT"
     CDS             complement(1216..1935)
                     /gene="btsR"
                     /locus_tag="ACHKAU_001840"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO5530918.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1932..3617)
                     /gene="btsS"
                     /locus_tag="ACHKAU_001841"
     CDS             complement(1932..3617)
                     /gene="btsS"
                     /locus_tag="ACHKAU_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO5530919.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3839..4570
                     /gene="mlrA"
                     /locus_tag="ACHKAU_001842"
     CDS             3839..4570
                     /gene="mlrA"
                     /locus_tag="ACHKAU_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO5530920.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4630..4737
                     /gene="yohO"
                     /locus_tag="ACHKAU_001843"
     CDS             4630..4737
                     /gene="yohO"
                     /locus_tag="ACHKAU_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO5530921.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4718..5449)
                     /gene="yehW"
                     /locus_tag="ACHKAU_001844"
     CDS             complement(4718..5449)
                     /gene="yehW"
                     /locus_tag="ACHKAU_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO5530922.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5454..6380)
                     /gene="yehX"
                     /locus_tag="ACHKAU_001845"
     CDS             complement(5454..6380)
                     /gene="yehX"
                     /locus_tag="ACHKAU_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO5530923.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6373..7530)
                     /gene="yehY"
                     /locus_tag="ACHKAU_001846"
     CDS             complement(6373..7530)
                     /gene="yehY"
                     /locus_tag="ACHKAU_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO5530924.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7537..8454)
                     /gene="osmF"
                     /locus_tag="ACHKAU_001847"
     CDS             complement(7537..8454)
                     /gene="osmF"
                     /locus_tag="ACHKAU_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO5530925.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8665..10962)
                     /gene="bglX"
                     /locus_tag="ACHKAU_001848"
     CDS             complement(8665..10962)
                     /gene="bglX"
                     /locus_tag="ACHKAU_001848"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO5530926.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AELGKFNVFIGTDSARVKKGEFELL"
     gene            11158..12873
                     /gene="dld"
                     /locus_tag="ACHKAU_001849"
     CDS             11158..12873
                     /gene="dld"
                     /locus_tag="ACHKAU_001849"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5530927.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12911..13843)
                     /gene="pbpG"
                     /locus_tag="ACHKAU_001850"
     CDS             complement(12911..13843)
                     /gene="pbpG"
                     /locus_tag="ACHKAU_001850"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO5530928.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(14017..14604)
                     /gene="yohC"
                     /locus_tag="ACHKAU_001851"
     CDS             complement(14017..14604)
                     /gene="yohC"
                     /locus_tag="ACHKAU_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO5530929.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14774..15352
                     /gene="yohD"
                     /locus_tag="ACHKAU_001852"
     CDS             14774..15352
                     /gene="yohD"
                     /locus_tag="ACHKAU_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5530930.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15482..16243)
                     /gene="yohF"
                     /locus_tag="ACHKAU_001853"
     CDS             complement(15482..16243)
                     /gene="yohF"
                     /locus_tag="ACHKAU_001853"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5530931.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(16296..17732)
                     /gene="mdtQ"
                     /locus_tag="ACHKAU_001854"
     CDS             complement(16296..17732)
                     /gene="mdtQ"
                     /locus_tag="ACHKAU_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO5530932.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17956..18039
                     /gene="yohP"
                     /locus_tag="ACHKAU_001855"
     CDS             17956..18039
                     /gene="yohP"
                     /locus_tag="ACHKAU_001855"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO5530933.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(18427..19377)
                     /gene="dusC"
                     /locus_tag="ACHKAU_001856"
     CDS             complement(18427..19377)
                     /gene="dusC"
                     /locus_tag="ACHKAU_001856"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO5530934.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            19616..20014
                     /gene="yohJ"
                     /locus_tag="ACHKAU_001857"
     CDS             19616..20014
                     /gene="yohJ"
                     /locus_tag="ACHKAU_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO5530935.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            20011..20706
                     /gene="yohK"
                     /locus_tag="ACHKAU_001858"
     CDS             20011..20706
                     /gene="yohK"
                     /locus_tag="ACHKAU_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO5530936.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            20836..21720
                     /gene="cdd"
                     /locus_tag="ACHKAU_001859"
     CDS             20836..21720
                     /gene="cdd"
                     /locus_tag="ACHKAU_001859"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO5530937.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            21870..22589
                     /gene="sanA"
                     /locus_tag="ACHKAU_001860"
     CDS             21870..22589
                     /gene="sanA"
                     /locus_tag="ACHKAU_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO5530938.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            22592..22831
                     /gene="yeiS"
                     /locus_tag="ACHKAU_001861"
     CDS             22592..22831
                     /gene="yeiS"
                     /locus_tag="ACHKAU_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO5530939.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            23150..24388
                     /gene="preT"
                     /locus_tag="ACHKAU_001862"
     CDS             23150..24388
                     /gene="preT"
                     /locus_tag="ACHKAU_001862"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO5530940.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            24382..25617
                     /gene="preA"
                     /locus_tag="ACHKAU_001863"
     CDS             24382..25617
                     /gene="preA"
                     /locus_tag="ACHKAU_001863"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO5530941.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(25688..26698)
                     /gene="mglC"
                     /locus_tag="ACHKAU_001864"
     CDS             complement(25688..26698)
                     /gene="mglC"
                     /locus_tag="ACHKAU_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO5530942.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(26714..28234)
                     /gene="mglA"
                     /locus_tag="ACHKAU_001865"
     CDS             complement(26714..28234)
                     /gene="mglA"
                     /locus_tag="ACHKAU_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO5530943.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(28295..29293)
                     /gene="mglB"
                     /locus_tag="ACHKAU_001866"
     CDS             complement(28295..29293)
                     /gene="mglB"
                     /locus_tag="ACHKAU_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO5530944.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(29573..30613)
                     /gene="galS"
                     /locus_tag="ACHKAU_001867"
     CDS             complement(29573..30613)
                     /gene="galS"
                     /locus_tag="ACHKAU_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO5530945.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(30755..31912)
                     /gene="yeiB"
                     /locus_tag="ACHKAU_001868"
     CDS             complement(30755..31912)
                     /gene="yeiB"
                     /locus_tag="ACHKAU_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO5530946.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(31929..32597)
                     /gene="folE"
                     /locus_tag="ACHKAU_001869"
     CDS             complement(31929..32597)
                     /gene="folE"
                     /locus_tag="ACHKAU_001869"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO5530947.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            32855..33691
                     /gene="yeiG"
                     /locus_tag="ACHKAU_001870"
     CDS             32855..33691
                     /gene="yeiG"
                     /locus_tag="ACHKAU_001870"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5530948.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(33723..35714)
                     /gene="cirA"
                     /locus_tag="ACHKAU_001871"
     CDS             complement(33723..35714)
                     /gene="cirA"
                     /locus_tag="ACHKAU_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO5530949.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(36007..37476)
                     /gene="lysP"
                     /locus_tag="ACHKAU_001872"
     CDS             complement(36007..37476)
                     /gene="lysP"
                     /locus_tag="ACHKAU_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO5530950.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(37681..38562)
                     /gene="yieE"
                     /locus_tag="ACHKAU_001873"
     CDS             complement(37681..38562)
                     /gene="yieE"
                     /locus_tag="ACHKAU_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO5530951.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
BASE COUNT         9449 a        10294 c         9620 g         9219 t
ORIGIN      
        1 cagcggcttg ctgaacgtca gcgaaaaacg aaaccgctgt tgaaatccct ggaaagctgg
       61 ttgcgtgaaa agatgaagac cctgttcttc ggctctggcc atggtggtga gcggggagcg
      121 ctactgtaca gcctgatcgg gacgtgcaaa ctgaatgacg tggatccaga aagctacctt
      181 cgccatgtgc ctggcgtcat agcagactgg ccggtcaacc gggtcagcga actgcttccg
      241 tggcgcatag cactgccagc tgaataacac atccccgtca atacggccct cgctgtacgc
      301 ttacgaacgg cactgaaatt gccattacct atgtctacaa aggtgacaag gtgcttaagc
      361 aatcttctga aaccaaaatt caatttgctt ctattggtgc aaccaccaaa gaagacgctg
      421 ccaagacact tgagccgtta agcgccaaat acaaaaacat cgcgggtgtt gaagaaaaat
      481 taacctatac agatacctac gcgcaggaaa acgtgactat cgatatggaa aaagtggatt
      541 ttaaagccct gcagggtatt tcaggaatca acgtttctgc tgaagatgcc aaaaaaggta
      601 tcactatggc gcaaatggaa ctggtgatga aagccgctgg ttttaaagaa gtgaaataat
      661 cggttggcgg tcatgctcta aacatgaccg ccaatttttt agcctttttt cacatgctgg
      721 cgcgctgcca gtccacgcag aaaataacgt aaaaattgat cgccgcattc gcggaagttt
      781 ttatgatccg gtgcgcgcat catcgctgta atttccggca tcgaaacgcg gaactgctgt
      841 tcggtgagga tagccagaat gtcatcggtt ttcagcgaaa acgcgatgcg taattttttc
      901 agcacgatgt tgttattaat gcgacgttcc ggctccagtg ccggagcaga ctcatccttg
      961 ccgcgttttt cataaatcag gccattgagg aatgacgaca aaaaaatgtc cggacaacgc
     1021 tgaaaaccct cttcgtcttc tttacgtagc cagacggcga tctgttccgc ggtggcttcg
     1081 acattaccca gagccagaat acgcaccagg tcattattat tggctttcaa aatgtagcgc
     1141 acgctgcgca gaatatcgtt acttagcatg aggccttcag gtgttgatga ggcaaaaagc
     1201 cattttagca gtcttttaca ggccaatcgc ctcttttaag cttttcagat aacggcggct
     1261 gaccggcacg gttaagccat tacgcaaaat caactcggcc tggccgttat cttccagacg
     1321 aatctcctgt aaatgcgcga ggttaaccag atactgacga tggcagcgca gtagtggtgt
     1381 acgactttcc agggtacgta atgtcaattc ggtaaagccc tctttccctt cgtggctggt
     1441 aacgtagaca ccgctcatcc gactgctgac aaatgccaca tctttcattt gcagcaaata
     1501 aatccgacta tgccccgtac aagggataaa tttcagcgcc tgttgatttt ccggtaacag
     1561 cgaaacatcc tgcttgctgc gctcctgacg caatcgcgcc agcgttttct ccagtcgcgc
     1621 ttcatcaatt ggcttcagca gataatcaaa ggcatgttct tcaaaggctt taatagcgta
     1681 ttcgtcaaac gcagtgagaa aaacaatata cgggcgatgt tccgggtcaa gcatccccac
     1741 catttccaga ccactgatgc gcggcatctg gatatcgaga aacagcacat ccgggcgcag
     1801 tttatgcacc gcgccgatcc cttccacggc gtttgaacac tctccaacga tttcaatatc
     1861 gctctgctcc tgcaaaaata cgcgcaggtt ctcccgtgct aacggttcat catcgacaat
     1921 taagacttta atcatgcctc gtccctccat ggtagtcgta acgttattcg ggtgtaacta
     1981 tcaggctcac aggcgacgct tattccatag tcatcgccaa accgttcacg taaacgctta
     2041 tccaccagat tcatccccag cccactggca ttggttaccg gttgatacaa accggcattg
     2101 tcttcgatct ccagcatcaa atgttgcccc tcacgtcggg cgctgattgc cactcgccct
     2161 gtatccagca gttgtgatgt cccatgttta atggcgtttt ccactatcgg ttgcagggta
     2221 aacgcgggca attgctgctg ggataattct tgcggaatag caatgttgac ctgcaaccgc
     2281 gactggaagc gcgccttttc aatttgcaga taagcgttca catgttcaat ttcgtcggcg
     2341 agagtaacaa actccgaagg ccgctttaag tttttgcgga aaaaagtgga aagatactgc
     2401 accagctggc tggcctgttc gctgtcgcgg cggatcaccg ctttaatggt gttaagcgca
     2461 ttaaacaaaa aatgaggatt cacctgggcg tgaagcagtt tgatttctga ctgggtgagc
     2521 atcgcttttt gccgctcata ttgcccggca agaatctgcg ccgaaagcag ttgcgcaatc
     2581 ccctcgccca gcgtgcggtt tattgaactg aataaacggt ttttggcttc atacaatttg
     2641 atggtgccca tcacgcgctg attttcgcca cgcaacggaa ttaccagcgt cgaccccagt
     2701 ttgcattgcg gatgcaaaga gcaacgataa ggtacttcgt tgccatcagc gtagaccact
     2761 tcaccggttt caatcgcttt taaggtgtaa gtcgaagaaa tcggtttgcc gggtaaatgg
     2821 tggtcgtcac caattccggt aaaggccagc aatttctctc gatcggtaat cgcgactgca
     2881 ccaatatcca gctcctgata cagcacctga gccactttca tgctgttcac ttcgttaaac
     2941 ccctgtcgca aaatgccttc cgtcgaggct gccactttca gcgcagtggc agaaaaagca
     3001 gaagtgtatt tttcaaacat cgcgcgttta tcgagcaata tacgcataaa cagcgccgcg
     3061 ccgacggtat tggtgaccat cattggtgca gcaatattac tcaccagacg caccgcatct
     3121 tcataaggtc gggcgatcgc aaggatgatc agcatttgca ccatttcagc gacgaacgtg
     3181 acggcaccgg cggtaatggg gttaaagact ttatcagtgc gcccgcgacg gatcaggatg
     3241 ctgtgtacca ggccgccgag caatccttca acgatggttg agatcatgca acttagcgcg
     3301 gtcatgcccc ccatcgaata tcgatgtaag ccgccggtca gaccaaccag cccaccgacg
     3361 accggaccgc cgagtaagcc gcccattacc gcgcctatcg cacgggtatt ggcaatagaa
     3421 tcgtcaatgt gcaacccaaa ccaggtgccc atgatgcaga agatggaaaa gacgatgtag
     3481 cagagaaatt tatgcggcag acgaaccgtg acctgcatta acggtatgaa taatggcgtt
     3541 ttactcatta accacgcaat gactaaaaaa acgcacatct gctgaagcag cagcaacacc
     3601 agattaaaat cgtacatacc cgcaaaccac acttcccttt aaaacgcgta acatacattg
     3661 cctgcgttta actttctttg aactcttgca gaaaaatgag aattcgtgag tacgatcact
     3721 caaaatcgcc tggcaaaaat aaaatcaccc tatagatgca caaaaaacgg gcaaaactac
     3781 ctggttcgca aaactgcgtc taaagttaaa ccgggacctc gcgagcaagg gtgagacgat
     3841 ggcgctttac acaattggtg aagtggcgtt gctttgtgat attaaccctg tcacgttacg
     3901 cgcgtggcag aggcgttacg ggttgctgaa accgcaacgg acagacggcg gtcatcgact
     3961 gttcaacgat gccgatattg accgtatccg cgagatcaaa cgctggatcg acaacggcgt
     4021 gcaggtcagc aaagttaaaa tgctgctcag taatgaaaat gttgatgtgc agaacggctg
     4081 gcgcgatcag caagaaacat tactgaccta cctgcaaagc ggcaatctgc atagcctgcg
     4141 aacgtggatc aaagagcgcg gtcaggatta ccccgcccag acactcacca cacatctgtt
     4201 tattcctctg cgccgacgac ttcaatgcca acaaccgact ctccaggcgc tgctggcgat
     4261 cctcgacggc gtactgatca actacatcgc catttgtctg gcttcggcac gtaaaaaaca
     4321 gggtaaagat gcgctggtgg ttggctggaa tattcaggat accacccgtc tgtggctgga
     4381 gggctggatt gccagtcaac aaggatggcg cattgatgtc ctcgcccact cgctcaatca
     4441 actacgccct gaactattcg aaggtcgtac attgctggtg tggtgcggtg aaaatcgaac
     4501 ctccgcccaa cagcagcaac tcaccagttg gcaagaacaa ggccatgata ttttcccact
     4561 cggcatttaa tgattcgtta acaaatgcgc tttactgtac aatcctttcg ttaacataag
     4621 gagtgcatta tgcgcatagc taaaattggg gtcatcgccc tgttcctgtt tatggcgttc
     4681 ggcggaattg gtggcgtcat gctcgcaggt tataccttta ttttgcgtgc tggctaagcg
     4741 cctgcaccag cctttcaaac aggcggtctg cgatgatcgc cgccagtgcc accagtaacg
     4801 ccccctggat cacatacgcg gtattaaatc cgctaagccc gatgatgatg ggcgtaccca
     4861 gcgtgctggc ccctaccgtt gaggcgatcg tcgccgtacc aatgttgata atcaccgaag
     4921 ttcgcacgcc cgccagaatc accggagccg ccagcggtag ctcgacctta cgcagtcgct
     4981 gaccacgact cattcccata cctttcgcaa cttctgtcac gctggcatcg atcgctccca
     5041 gcccggcaag tgtcgcctgc aggacgggca gcacaccgta aaggatcaag gcgataatcg
     5101 ctggttgcag accaaagccg atcaccggaa cggcaatcgc cagcactgcg acaggcggaa
     5161 aagtctgtcc aacggcggca atagtttcca ccagtgggcg aaattccgcg ccccacgggc
     5221 gagtgacagc aattccggca ccagtgccaa tgatcaccgc aaacaaactc gaaattccca
     5281 ccagccagaa atgagccagt gccagagctg caaaactttc ttgctgataa acgggtcgtg
     5341 gcagttgtgg gaacaaggca gcaaacagcg gctggctgta aggcagccag aaaatcagtg
     5401 ccacaaacaa agcaatgagc cagaacagcg gatcgcgcaa catcttcata cgcttacgcc
     5461 tccaccagca gatcctgaaa atgcagcgta ccgcaaggct ggccctgcgt gttcaccacc
     5521 ggcagcacct cgcaaccccg cgcgacaaac agggagagcg catcgcgtag cgtcatctct
     5581 tctgccagtg cctcaccttc tgcccgttct tcgcgacgca cgtaatccgc cacactacgt
     5641 aacgaaagca gacgcacacc cagttcgcta cgtccaaaaa actggcggac aaaatcattc
     5701 gccggacgag tcagcatcgt cagcggattg ccctgctgca ccacttcacc gtgatccatc
     5761 aataccagat gttcagccag ccgtagcgcc tcatcaatat catgagtgac cagcacaata
     5821 gtgcgcccca gcaaacggtg aatgcgcgtc atctcttgtt gcaacgcgcc gcgcgttacc
     5881 gggtccagtg cgccaaaagg ttcatccatc agtaagactt gcggatcggc agccagtgcg
     5941 cgcgccactc ccacacgttg ctgctgacca ccggaaagct gatgcggata acgctcacgc
     6001 aaatttgact ccagccccag tagcgtcatt aattcgtcga tacgatcgtc aatccgtgcc
     6061 cgcgaccatt tttgtaattg cggcacggta gcgatgtttt gcgccacgct ccagtggggg
     6121 aataggccaa tagattgaat ggcatagccc atccggcggc gcaactccag tactggcagc
     6181 gagcgaattt cttctccggc aaagcggatc tctccgctgt catgttccac caggcggtta
     6241 atcattttca aggtggtgga tttgccggag ccagatgtgc caatcagcac cgaaaaactc
     6301 ccttcctgaa aattgagatt aagatcgtta acggcttttt gtgcgccaaa cagtttgctg
     6361 acatggctaa attcaatcat tacgtttcac cttcagcagt gcgataagca aatcgaacag
     6421 ggcatcgatc agcaccgcca gaacaattac cgggatcacc cccagcaaca ctaaatcaat
     6481 ggcgctgctt agcagcccct ggaaaaccag cgcaccaaaa ccgcctgcgc cgattaacgc
     6541 cgcaatcacc gccataccta cagtttgcac catcaccacc cgcaggctgc gcagaaatac
     6601 cggtaacgcc agtggtaact gaacatgcag gaatcgctgc gccccgctca tgcccatcgc
     6661 tctggcgctc tccagcacat cgcgcgggat ctggttcaaa ccgactacca cgccgcgcac
     6721 cagcggcagc aaggcataga gcaccagcgc aatcagtgcg ggtgtcattc cggttcctgc
     6781 tatgccgagc ttccccagcc acggaaaggc cgtcaccagc gcggcaagcg gcgcaatcaa
     6841 caggccaaag agcgccaccg aaggcacggt ctgaatgaca ttgagcagag aaaaaattgc
     6901 cccctgccga gcagtggaaa agtagcacca gatgcccaac ggcacaccaa tcactaacgc
     6961 aggcagcacc gcaccaaaca gcaacgtcag atgttgtgcc agcgcgtcgt caaacacatc
     7021 ctgacggttg gcgtattctt tcattagtga gagatcgtta agcgtgccgg agtacagcaa
     7081 ccacagcgga ataatggcaa tctgcatatg caacaaccag cgccacagcg gatgcgtgga
     7141 gattcggcgg atggcatcgc tacaggccag caatgccagc gccgcagcca gccagaaacc
     7201 actgccgagg ctggtacgcg ccagcgcact gccattttgc gccagttgtg tcgccgcctt
     7261 tcccgcgccc cacaccagca atacgaagac gaattgcgcc agaatgagtg cacaaatgct
     7321 gcctttttta ccgggaataa aacaggccgt cagccaggcg caaccaacgc ccaccagcat
     7381 ccagatcgtt tgcggccaca gttgccagag atgacgccct tcacccgaga ctaaacgatt
     7441 aggcgcgtag ctgataaacg gcagcgctgc tgcgattgcc gtcagcaaca gcagcagcgc
     7501 cagaacagga ttaatacgta gataagtcac gggaaattac ttcgcccacc ctttttgttt
     7561 caggtagtcg gcagccactt ttttggcatc cagtccttcc actgcaatgc tggcattcag
     7621 ttgctgcaat gtttttgcat cgaggctggc gaagactggc tgtagccact gtgccatttg
     7681 cggatactcc ttcaacaccg attcacgcac cactggtgca ggcgcataga taggttgcac
     7741 gccttgcgga tcgcttaagg tttgcagccc cagcgccgct accgagccgt cagtgccgta
     7801 agccattgca gcattaacgc cagaggtttg ctgggcagcg gctttgatcg tcaccgccgt
     7861 gtcgccacca gccagtgaca gcaactgatc ctgaccgagc ttaaagccat aggctttttc
     7921 aaacgcgggt aacgcatcgg cgcgttcgat aaactctgcc gaggctgcca gtttgaaggt
     7981 gccgccctct tgcagataac gactcaggtc ggcaagcgaa gtgagtttat ttttctctgc
     8041 cacatcctga cgcacggcga tagtccaggt gttatttgca ggcgcgggcg tcagccagat
     8101 taacttgttg tgctccgaat cgagcttttt gactttctcg taaccttgct gcgcgttttt
     8161 ccacgctgca tcgttttcat ctttaaagaa gaaagcgcca ttgccggtat attccggata
     8221 gatatccagt tcaccggaag taatcgcccc gcgcaccaca ggagtcgttc cgagttgcac
     8281 tttattcacc gtcggtacgc cgtggctttc cagcacctgc aaaatgatat tgccgagtag
     8341 cgcaccttcg gtatcgattt ttgaaccgac tttaacgggg gaagccgcct gtagcggcag
     8401 gctcacggct gccaacataa ccagtgaacc tgcccagagc tttaagagtg gcatgatgct
     8461 ttcctcattc ttttactgtt gttttcagcg aattaagaga aaagcatagt tgataatggc
     8521 ggggttagct tgagcggaag gattcagttg cagaatcaga taaatactta agaggcatat
     8581 tcggtccggc gtttcctgcc ggatgcggcg cgagcgcctt atccggccta caaagggcgc
     8641 aaacgtcgcg cccttactaa agcattacag caactcaaac tcgcctttct taacgcgtgc
     8701 ggaatcagtg ccgataaata cattgaactt gccaagctcg gcgtcatatt tcatctgttg
     8761 attccagaac ttcagcgcct caatatcgat cgggaagctg acagtctgag tttcgcccgg
     8821 tttcagggtg attttctcaa agcctttcag ctgtttcaca gggcgactca tggaagccgt
     8881 cacatcctgc aagtacatct gcactaccgt ggccccctcg cgcttaccgg tgttcgtcac
     8941 ctgcacgctg gcagtcactt tgccgtcacg cttcatggtc ggcgcagaaa gtttcacatc
     9001 agagacggtg aaagtggtgt agctcagccc atagccgaac ggatacaacg ccccgttagc
     9061 ttcatcaaaa taacgcgaag tgtatttgtt cggcttgtcg gcattatacg ggcgaccggt
     9121 attcagatgg ctgtagtaca ccgggatctg cccgacagaa cgcgggaagg acattggcag
     9181 cttgccggac gggttgtaat cgccaaacaa tacatcggca attgcattac cgccttcagt
     9241 ccccgcaaac caggtttcca gaatcgcatc agcctgctga tcttctttca ccagcgccag
     9301 cggacgcccg ttcatcagca ccagcaccag cggtttaccg gtggctttca gcgccgcaat
     9361 caagtcacgt tggctttgcg gaatagtgat atcggtccgg ctggaggctt cgtgcgccat
     9421 cccctgtgct tcaccgacta cagccaccac cacatcagat tgtttcgccg tctgcaccgc
     9481 ttcatcaatc atctcttgcg gcgaacgcgg atcgactttg accgcttctt catactgatt
     9541 caggaaatcg ataatgcctt tgtcactggt aacgttcgcc cctttggcat acagcacttt
     9601 accgttttca ccgacggaat ttttaatccc ggtcagtacg gtcacggatt gatcggcaac
     9661 accggctgcg gaccagctgc ccatcacgtc acgtttactg tccgccagtg gcccaaccac
     9721 cgcaatggtg gccgattttt tcagcggtaa cgtttcgaga cggtttttca gcaacaccag
     9781 gctttcgcgc gccacttcac gcgcttcttt acggtgcagg cggctttcgg cattggtatc
     9841 caccgggtca gactctttcg gccccaaatg gctgtatggg tcgttaaaca atcccatatc
     9901 atatttaacg ttcagtacat ggcgggcagc atcgtccagc tcttccatcg tcactttgcc
     9961 ggatttgatc aacccaggca gatacttcga gtaatactcg tcgctcatac tcatgttgat
    10021 tccggatttc agcgccacgc gcaccgcatc ttccgggtct gccgccgtgc catgtttaat
    10081 cagctctttg attgcaccgt gatcggaaac ggtgatgcct ttaaagcccc actggtcgcg
    10141 cagaacatct ttcagcagcc aggaatcgga ggtggctggc gtgccgttca gcgagttcag
    10201 cgccaccatc accgcgccgc tgcctgcgtc cagccccgct ttgtacggcg gcatataatc
    10261 attaaacagg cgctgcggac tcatatcgac ggtgttgtac tctttaccgc cttctaccgc
    10321 gccgtatgcg gcaaagtgtt tgacgctggt catcaccgag tagcgatctg ccgggctttt
    10381 accctgcatc gcttccacca tggttttacc cattgttgag gtgagatacg tatcttcgcc
    10441 aaaaccttcg gaagcacgtc cccagcgcgg atcgcgcgag acatcgacca tcggtgccca
    10501 ggtcatattc aggccatcat ctgccgcttc ataagcagag acacgtccga ccgttttcac
    10561 tgcatcgagg ttaaaagacg aggccagacc gaggctaatc gggaacaccg tgcgctgacc
    10621 gtgcagcacg tcgtaagcaa agaaaagagg aattttcagg cggcttaatt ccatcacctg
    10681 atcctgcatg gcgcggatat cctgacgggt tacggtgttg aaaatcgccc caacctgacc
    10741 gtctttgatc atctcgcgga tcgcctcttt cgggttatcc gggccgacgc tgattaagcg
    10801 cagctgacca attttctcat caactgtcat tttcttaagc agttcggtga cgaacgcatc
    10861 ccgcgcttcg ggcgttaatg gatggttgcc gaataaatca tccgccagtg ctggctgcag
    10921 ggccagactc accgcgattc ctactgaaca tagccatttc atatggattt ttcttcctct
    10981 gttgcccgac gaggcagcga aaatggtgca aaaaccgtag tttgccataa gcatgatgga
    11041 gagagaaaaa gaatgctcag tttattgtct gaattttcaa aatattcact cgctgaattg
    11101 tcatacaagg cgctattcta gtttgtgata ttttttcgcc accacaagga gtggaaaatg
    11161 tcttccatga caacaactga taataaagcc tttttgaatg aacttgcccg tctggtcggt
    11221 cattcacacc tgctcaccga tcccgcaaaa acggcccgct atcgcaaggg cttccgttct
    11281 ggtcagggcg acgcgctggc tgtcgttttc cctggctcac tactggaatt gtggcgggtg
    11341 ctgaaagcct gtgtcaccgc cgacaaaatt attctgatgc aggctgccaa tacaggcctg
    11401 accgaaggat cgacgccaaa cggtaacgac tatgatcgcg atatcgtgat catcagcacc
    11461 ctgcgtctcg acaagctgca cgttcttggc aagggcgaac aggtgctggc ctatccgggc
    11521 accacgctct attcgctgga aaaagccctc aaaccgctgg gacgcgaacc gcactcggtg
    11581 attggatcgt cgtgtatagg cgcatcggtt atcggcggta tttgtaacaa ctccggcggc
    11641 tcgctggtgc aacgtggccc ggcgtatacc gaaatgtcgt tattcgcgcg tataaatgaa
    11701 gacggcaaac tgacgctggt gaaccatctg gggattgatc tgggcgaaac gccggagcag
    11761 atccttagca agctggatga tgatcgcatc aaagatgacg atgtgcgtca cgatggtcgt
    11821 cacgcccacg attatgacta tgtccaccgc gttcgtgata ttgaagccga cacgcccgca
    11881 cgttataacg ccgatccgga tcggttattt gaatcttctg gttgcgccgg gaagctggcc
    11941 gtctttgcgg tacgtctaga taccttcgaa gcggaaaaaa atcagcaggt gttttatatc
    12001 ggcaccaacc agccggaagt gctgaccgaa atccgccgtc atattctggc taacttcgaa
    12061 aatctgccgg ttgccgggga atatatgcac cgggatatct acgatattgc ggaaaaatac
    12121 ggcaaagaca ccttcctgat gattgataag ttaggcaccg acaagatgcc gttcttcttt
    12181 aatctcaagg gacgcaccga tgcgatgctg gagaaagtga aattcttccg tccgcatttt
    12241 actgaccgtg cgatgcaaaa attcggtcac ctgttcccca gccatttacc gccgcgcatg
    12301 aaaaactggc gcgataaata cgagcatcat ctgctgttaa aaatggcggg cgatggcgtg
    12361 ggcgaagcca aatcgtggtt ggtggattat ttcaaacagg ccgaaggcga tttctttgtc
    12421 tgtacgccgg aggaaggcag caaagcgttt ttacaccgtt tcgccgctgc gggcgcagca
    12481 attcgttatc aggcggtgca ttccgatgaa gtcgaagaca ttctggcgtt ggatatcgct
    12541 ctgcggcgta acgacaccga gtggtatgag catttaccgc cggagatcga cagccagctg
    12601 gtgcacaagc tctattacgg ccattttatg tgctatgtct tccatcagga ttacatagtg
    12661 aaaaaaggcg tggatgtgca tgcgttaaaa gaacagatgc tggaactgct acagcagcgc
    12721 ggcgcgcaat accctgccga gcataacgtc ggtcatttgt ataaagcacc ggagacgttg
    12781 cagaagttct atcgcgagaa cgatccgacc aacagcatga atccggggat cggtaaaacc
    12841 agtaaacgga aaaactggca ggaagtggag taaaaattac ggatggcaga gtatcgccat
    12901 ccgaattcac ttaatcgttc tgtgccgtct gccccgccgc cgccatttgg gcggcttttt
    12961 gttttttata gctcaacgct gctgccggca caggcatcac tttaccggtt tcaatccagg
    13021 tacgcaggcg gctggcatcg gcaaaatggg tatatttgcc aaacgcgtcc atcactacca
    13081 gcgccaccgg tttattattg ataaccgtac gcatcaccag acaatggccc gccgcattgg
    13141 taaagccggt tttggttaac tgaatattcc agttatcgcg atacaccaga tgattagtat
    13201 tgcggaacgg cagcgtatac gtcgggttag agaaggttgc catatcttcc cgggtagtac
    13261 ttaactgccc gatcaacgga tactgtttgc tggcaatgag cagtttggtt aagtcacggg
    13321 cagttgaaac gttatgcacc gacaatccgg taggttcaac aaagcgcgtg ttgttcattc
    13381 cgagcgattt cgctttcgca ttcattgcct taataaaggc tttgtaacca ccgggataat
    13441 ggtgcgcaag gcttgccgcc gcgcggtttt ctgaagacat cagcgccagc aacagcatat
    13501 ctttacggct gatttcgcta ttcagtcgta cgcgcgaata gacccctttc atctccggcg
    13561 tctggctgat atccactttt agtttttcat ccagcggcag tcgtgcatcc agcacaacca
    13621 tcgcggtcat taatttgctg atagacgcaa tcggacgcac cagatccggg tggttcgaat
    13681 agatcacttt gttggtattc agatcaacaa tcatcgcgct accggaggca atttccggtt
    13741 gtgaagcggt ggtagcggct gccgttttcg caacggcctg cggtgccaaa ggcacagcca
    13801 gcatcagggc caggctaaat aaagaaactc gaaatttcgg catgatgagc attcagatag
    13861 tggttcacgc gcacgggttg cgcaccgccg gagtaaggat ttactgaggc tagcgacgcc
    13921 atcataacga gcaaaaagtg cgatcgtcaa aggagaatcg tgaggaaatg ctgcattgct
    13981 gacatttacg ccagcaatgc aatgtcaaag aactttctag aacaggcgat acccgtagcc
    14041 ccatagtata acggttaggg cgagcagcac ttccagtacc agcacgccaa tcgccagcgt
    14101 cgaactggag aagctaaggc cttcctcttt gttgatattc aggaagctcg gaataccaag
    14161 gtaaagcagg tagccggtgt aaaacagcgc caccgtgccg accagcgcac acaaccagac
    14221 cagcggataa agcgccacca gaccgcttaa aaacagcgga gttgcaacgt agcccgcgaa
    14281 gaccatgcag tgcgcaagtg acggacgctg cgggtaattg cgcgccatcc accagatgac
    14341 ccgccccatc accgcaaccc cagcgagcat aacgccataa aacaggacag ccagcgccag
    14401 tccggtaaac caggacaact tcaggatagt gccatcgcca aaattccagc caatctgtgt
    14461 agtgccaatg aaggcgcaaa tcaccggaat cgccgccatc agcaaaacgt ggtgggtgta
    14521 atggtgagaa atcgtttcgt tttcgcgatt aatcacctgc atttcacgat cgggatggga
    14581 aaacagtccc cagacatggc tcataccgcc cccttgttgt gagttcatga acctgacagt
    14641 tcaagtataa gtcagcttgt gattattttt tgttcgctca tgtaatttgc accgtctgat
    14701 gtacgtcctt tttcagcgtg aatgatttcc agggtgtatg cttacaggca accaaaggga
    14761 gacagactgg cctatggatc tcaatacact catctcacaa tatggttatg ccgcgctggt
    14821 gatcggtagc ctggcggaag gtgaaaccgt gactttgctg ggaggcgttg cggcgcatca
    14881 ggggctatta aagttcccgc tggtggtact ttctgtggcg cttggcggca tgattggcga
    14941 ccaggtgctt tatctgtgcg ggcggcggtt tggcggcaag ctgttacgcc gtttctcgaa
    15001 acatcaggat aaaattgagc gggcgcagaa acttatccaa cgccatccgt atctgtttgt
    15061 cattggtacg cgctttatgt atggctttcg ggtgattggc ccgacgctga ttggtgccag
    15121 ccagctgccg ccgaaaatct ttctgccgct taatattctc ggcgcatttg cctgggcgtt
    15181 gatttttacc actatcggtt acgctggtgg tcaggtgatt gcgccgtggt tgcacaatct
    15241 cgatcagcat ttgaagcact gggtctggtt gattctggtc gtggttctgg tggtaggcgt
    15301 gcgctggtgg ctgaaacgac gcgggaagaa aaagccggat aatcaggcgt aaaaccattg
    15361 ccctggataa ggcgttcgcg ccgcatccga catccggtgc accatgcctg atgcggcgct
    15421 tgtgcgtctt atcatgccta ccaaaatttt acccattgaa atatccacac acctcactgc
    15481 gctattctgg gttgaactgc ggattcgcca acataaagcc gccatccact atcaacgact
    15541 gcccggtggt gtaatttgcg ccctccgaac aaagccacgc caccaggctg gcaatctcat
    15601 acgttgcgcc aaaacgccgc agaggaatcg aaggctccgc gtcgggcttc acgtcgctat
    15661 catccatgcc gttcattggc gtagcgatcg ccccaggtgc gactgcgttc accaaaatct
    15721 tatgcctgac caactccagc gccattgctt tggttaaccc gccgagcgca tgtttagcgg
    15781 ctgtgtaggc gctggcatcc ggcagcggcg tatgttcatg taccgacgta atgttgatga
    15841 tgcgaccacc ctgcccttgt ttcaccatct ggcgggccgc aatttgtgag cataaaaatg
    15901 caccatcgac atcaacggta aaaatcttgc gccactcatc gaaagccata tcaagaaacg
    15961 gcgctttggt cattgcgccc gcattattca ccagcacatc aatacgcccc agccgttgaa
    16021 tgagtttctc cagcgcctgt gcaccttctg gcagtttacc gagatccagc tgcacaatct
    16081 ccgcacgtac gccgtggcta actacctcac gcgcggtatc ttttgcccct tcttcatctg
    16141 agtgccaggt aatgccaata tcaaacccct gctgcgccag taataacgcg cactctttgc
    16201 cgatccccga atcggaggcg gtaataatcg caacctgtgc catcgagttc tccacttaac
    16261 gctgaataaa cgttaagtat agaaggcgca tatcatcagc gtttgtaacc cccgcccaac
    16321 gcaccagtga gttgaatgga agcatccagc cactgccctt gcaataacag gccattggcc
    16381 cgctcacgca gcgcggggat tctggcttcg ctgacgcggg aaccagcaat gatgcccgcg
    16441 ttaaagcgcg cctgcgcaag acctaccaca cgcaaggcat cgcgctcaat ttgcgcctga
    16501 tgctggtttt tctccgcaag tgtctgaacc tgactggctg cccgcgccac gtcattcacc
    16561 gcttcaacca ccgctttgtt gtagctggcg atagacaagt tgctttcggc ttttgcgata
    16621 tcgagattgg cgttaagacg accactatcg aaaatgggta gcgtcaggcc tgccgtaacg
    16681 cccatttgct gcgcggaatg acggaacaga tcgcttaagt gcaacgcatc ctgttgcagg
    16741 aaggccatca ggttgatgtc aggataaaat gccgcttttg ccgcatcaat ggtgcttagc
    16801 gatgactcaa cgtaccagtg cgccgcctgc aaatctgccc gccgggccag taaggagtac
    16861 cccagttcat caggaagctg gcttgccact ttcggcaacg cgaccggttt aagcttcaat
    16921 gactttgtct ggttatttgt aagtgcgctt aaccgtgcct caataatttt cattttcccc
    16981 gcgacatcgt tgagctgctg ccgggttttg ctggcattaa tatcggtttc cacaccttca
    17041 actgaagaag taatcccgtt ctgatatagc tggcgatcgg tcgcgataat ggtgttctgc
    17101 tctttttcta tttgctgcaa gaccgtgttt aacgccgcct gggtttgcca ctcccagtac
    17161 aggcgggcta cgctgccagc cagcaattgg cgggtttgct cgcgttccgc cgcccgtgct
    17221 ttaaccgtac ccaggcgggc agtaacctcc gcccgattct ttccccagat atcgagatgc
    17281 cagcccgccg ttaagccaaa agttccgttg gtgtaccacg gaccggtcgt acctgcggcc
    17341 ggatcgttca gagcaaacgg ccccattaag ccttctgccg acattttttg ccgctccata
    17401 tccgcagaaa agtcgatctg cggaccatcc tgagtagcaa ttgccttcgc ctgggcttca
    17461 gctagctgaa tgcgctgttc agccacctgc atatccggtg cgttctgtag tgcattgtta
    17521 attaaggaag tgagttgatt atcgtgatac tccagccacc attggctgtc tggccaacca
    17581 tttttcagcg ccgtgggtaa tgcggtgtca acttgtgcag cgggcgtttg ctggcttaac
    17641 gcctggcggg tttcatgcat aggcgcacac ccggccagca tcagtaacag cggaaaacag
    17701 gcgatggcgg gataaaagga atcacgattc atgggggaat aatcaggtaa gaaaaggtgc
    17761 gcggagatta ccgtgtgttg cgatatattt tttagtttcg cgtggcaata catcagtagc
    17821 aataaaacga catatccaga aaaatataca ctaagtgaat gatatcttcc gatttatctt
    17881 aatcgtttat ggataacggc aaagggcttc gttttttcct atacttattc agcactcaca
    17941 aataaaggaa cgccaatgaa aattatactc tgggctgtat tgattatttt cctgattggg
    18001 ctactggtgg tgactggcgt atttaagatg atattttaaa aatcagatat ggcatataag
    18061 ttgtcaggtc aggaactaga tattttgtgt acttgatttg caaacagctc cggtgttggt
    18121 ggcgtcaaaa tcgctgaagc gttccccgaa ggccggggca gcccacatgg atgtgggctg
    18181 agggcgcgtc ttacaaggac gttacctcgc gcccgacccg gtagacgaag gggataagct
    18241 gaaggtaccg cgcagcggcg attttgttcg ccagagcccg ggggtgcatg gggcggcggc
    18301 gattggccgc cccctgcgcg ctccttgcgc cagtggcaat atgtggctta gctcatgaaa
    18361 ggagcgcaac aagatgatga agctgaataa gcgattcatt gtctctgata aactgcgatt
    18421 ccactattat cgcagcttat cgatatcaat tgcctgaata gcccttgcaa tatcagggga
    18481 attattcaac acccgaacat gctgaaataa ttccgttgct tcatcgtatt ctttacgcaa
    18541 ataactcaac cactgtttaa tccgcgcaac gtgatataac ccggtatcgc cctgcttttc
    18601 cagacgggta tatttttgca gcaaagcaac cacctccggc cacggcattc gcggttcgtt
    18661 atattttact acccggctca ggttgggaat attgagcgcc ccgcgaccaa tcatcactgc
    18721 gtcgcagccg ctgatcgcaa tgcattgttg cgcgctctgc cagtcccaga tttcgccgtt
    18781 ggcaatcacc ggaatattca gccgctggcg aatctcgcca atcgcctgcc agtcaatatg
    18841 ctccgcgcgg taaccctgct cttttgtccg tccatgcacc accagctccg tagcaccagc
    18901 ctgttgaacc gcatcggcga tttcaaattt cttctcaccg ctgtcccagc ccagacgcac
    18961 tttcacgctg acgggcaaat gcgccggtac agcttcacgc atcgcttttg caccctggta
    19021 gatgagttca ggatctttga gtaacgtcgc cccaccaccg ctaccgttga ccgttttcga
    19081 cgggcagccg caattgagat ccacgcccca ggaacctaat tccaccgcac gggcggcgtt
    19141 ctctgccagc cattgtggga actgacccaa caactgcacg cgcaccagcg taccggatgg
    19201 tgtccggctg gcgttttgta gctcagggca aatgcgatga aaaactttta ccggcagcag
    19261 ttgatccacc acgcggacaa actcggtgat gcacagatcg tagtcgttaa cttcggtcag
    19321 caattcacgc accagagagt caagcacacc ctccatcgga gccagtaaca cacgcatatc
    19381 ttcacccgca aaaaaatgag gcgctatgtt agcgcctatg gtcaacggat taaaggtctg
    19441 caaagaggga ttgctgcaaa ctgtaatccc tctctatgct tgatgtatgc ctgagttcgt
    19501 tgcgcattgc gtaatgccgg ggagcgaaaa aataagcgct gatttcttaa tgtgatcggt
    19561 agcacgtttt acgaattaat tgtatgatga atccatctca tctggggtgt tgattatgag
    19621 taagacactg aacattatct ggcaatattt acgcgctttc gtcctgattt atgcctgcct
    19681 gtatgcaggc attttcattg cttccctgct accggtaacc attccgggca gcatcatcgg
    19741 tatgctgatc ctgtttgtcc tgctggcctt gcaaattctt ccggcaaaat gggtcaatcc
    19801 ggggtgctac gtactgattc gctatatggc gctattgttt gtgccgattg gcgtaggcgt
    19861 catgcaatat tttgatttgc tccgcgcaca gtttggcccg gtagtggttt cctgtgcaat
    19921 cagtacgctg gtggtttttc tggtggtgag ctggagttcg caactggtac acggtgaacg
    19981 taaagtcgta ggtcagaaag gatcagaaga atgatggcaa atatctggtg gtcattaccg
    20041 ctgacgttga ttgtcttttt tgccgcccgc aaactggcgg cacggtataa gtttcccctg
    20101 cttaacccgt tgctggtagc aatggtggtg atcattcctt ttttaatgct gaccggcatc
    20161 tcttacgaca gctactttaa aggtagcgaa gtgctaaacg atctgctgca accggcggtc
    20221 gtcgcgctgg cctatccttt atatgagcag ctacaccaga tccgcgcgcg ctggaaatcg
    20281 atcatcacca tctgttttat cggcagcgtg gttgcgatgg tgacggggac ttccgtggca
    20341 ttgttgatgg gcgcttcacc ggaaatcgcc gcgtcaatcc tgccgaaatc agtcaccacg
    20401 cctattgcaa tggcggttgg cggcagtatt ggcggtattc cggcaatcag cgcagtttgc
    20461 gtgattttcg tcggcatcct cggcgcggta tttggccata cattgcttaa tgcgatgcgt
    20521 attcgtacca aagctgcgcg cggtctggca atggggactg cctcgcacgc cctcggtacg
    20581 gcgcgctgcg ccgagctgga ttatcaggaa ggtgcattta gttcgctagc gctggtgtta
    20641 tgcgggataa ttacttcgct gatcgcaccg ttccttttcc cgattattct ggcagtaatg
    20701 ggctaaaatt tgcgatgcgt cgcgcatttt tgatgtaagt ttcacgcgtt gcataattaa
    20761 tgagattcag atcacatata aagccataac gggttcgtaa actgttatcc cattacatga
    20821 ttatgaggca acgccatgca tccacgtttt caaaccgctt ttgcccaact tgcggataac
    20881 ttgcaatctg cactggaacc tattctggca gacaagtact tccccgcttt gttgaccggg
    20941 gagcaagtct catcgctgaa gagcgcaacg gggctggacg aagacgcgct ggcattcgca
    21001 ctacttccgc tggcggcggc ctgtgcgcgt acgccattgt cgaattttaa tgttggcgca
    21061 attgcgcgcg gtgtgagcgg aacctggtat ttcggtgcca atatggaatt tattggtgcg
    21121 acaatgcagc aaaccgttca tgccgaacaa agcgcgatca gccacgcctg gttgagtggt
    21181 gaaaaagcgc ttgcagccat caccgttaac tacacgcctt gtggtcactg ccgtcagttt
    21241 atgaatgaac tgaacagcgg tctggatctg cgtattcatc tgccgggccg cgaggcacac
    21301 gcgctgcgtg actatctgcc ggatgccttt gggccgaaag atctggagat taaaacgctg
    21361 ctgatggacg agcaggatca cggctatgcg ctgactggtg atgcgctttc tcaggcggcg
    21421 attgcagcgg caaaccgttc gcacatgcct tacagtaagt cgccaagcgg tgtcgcgctg
    21481 gaatgtaaag acggtcgtat tttcagtggc agctacgctg aaaacgccgc attcaacccg
    21541 actctgccac cgttgcaggg agcgttaatt ctgttgaatc tcaagggtta tgattacccg
    21601 gatatccagc gcgcggttct ggcagaaaaa gccgatgcgc cgttgattca gtgggatgcc
    21661 acctccgcaa cgctgaaagc tctcggctgt cacagtatcg accgagtgct tctcgcttaa
    21721 gcctggtgcc ggatgcggcg tgaacgcctt gtccggcttg ccagccctct cctggtgtcg
    21781 aaattcccgg caaacagttt gccgtttctt gcgcaaaacc agcgggtaaa gtagcctgat
    21841 ggaaattttc cttagatcga gtctcctgca tgttaaagcg cgtgttcctc agcctgttag
    21901 tcctgatcgg cttgctgctg ttgactgtgc tcggcctcga tcgctggatg agctggaaaa
    21961 ccgcgcctta tatctacgac gaattgcagg atctccccta ccgccaggtc ggtgtggtgc
    22021 tcggaacagc aaaatattat cgtactggcg taattaatca gtattatcgc taccgcattc
    22081 aaggagcgat taatgcctat aacagcggta aggtaaatta tctattactg agcggcgata
    22141 acgcattgca aagttataat gagccgatga ccatgcgcaa agatttaatc gctgctggtg
    22201 tcgacccatc agatattgtt ctcgattacg caggctttcg tacgctggat tccatcgtgc
    22261 gtacacgcaa agttttcgat actaatgatt tcattattat cacccaacgt ttccactgtg
    22321 agcgggcatt atttattgcg ctgcatatgg ggattcaggc tcagtgttat gccgtaccgt
    22381 caccgaaaga tatgctgtca gtacgtattc gcgaatttgc cgcccgtttc ggtgcgctgg
    22441 ctgaccttta tatttttaaa cgtgaaccgc gttttttagg gccgctggtc cctattccgg
    22501 ctatgcacca ggtgccggaa gatgcgcagg ggtatcccgc cgtcacaccc gaacagttac
    22561 ttgaattaca aaagaaacaa ggaaagtagt tatggatgta cagcagtttt ttgtcgttgc
    22621 cgtttttttc cttattccga tattttgttt ccgcgaagca tggaaaggct ggcgcgcagg
    22681 cgcgattgat aaacgggtta aaaatgcacc ggaaccggtg tatgtctggc gagcaaaaaa
    22741 tcccggactc tttttcgctt atatggtggc atatatcggc ttcggaattt tatctatcgg
    22801 catgattgtt tatcttattt tctatcgtta atattttctc ttttctgtta attattgaga
    22861 ataattatta cttcactcaa taagctgcgg atataacatt ctgaaccgca gctatttgtg
    22921 aatcctttca cagtataaat ttccccacgc gcatagcctt aatatcagca cgcaatattt
    22981 atactctaaa taattcgagt tgcaggaagg cgacaagtga gtgagtcccc aggagcttac
    23041 ataagtaagt gactggggtg agcgaacgca gacgcagcac atgcaacttg aagtatgacg
    23101 agtatattta gcgttcgcct taattactca tagcattaag gaagatcaca tgccgcaaca
    23161 aaattatctg gatgaactca ctccggcttt tacgccttta ctggcgatta aagaagcctc
    23221 tcgctgttta ttatgtcatg acgctccctg tagtcaggct tgcccggcac agaccgatcc
    23281 ggggaaattt attcgctcaa tctactttcg taattttaaa ggcgctgccg agacaattcg
    23341 cgaaaataat gccctcggtg ccgtttgtgc cagagtgtgc ccgacggaga aattatgtca
    23401 aagcggttgt acccgtgccg gtgttgatac tcccattgat atcggtcgct tacagcgttt
    23461 tgttactgat tttgaacaac aaaccgggat ggaaatttat cagcccggta ctaaaacgct
    23521 cggcaaagtc gcgattattg gcgcaggtcc tgccggattg caggccagtg taacactgac
    23581 aaaccagggt tatgacgtca cgatttatga gaaagaagcg caccccggtg gctggttgcg
    23641 taacggtatt ccgcaattcc ggttaccgca atcagtgctg gatgcagaga tcgcccgtat
    23701 tgaaaaaatg ggcgtgacca ttaagtgcaa caacgaagtc ggtaacacac tcacccttga
    23761 gcagctgaaa gcagaaaacc gcgcggtact ggtcaccgtg gggttatcga gcggttccgg
    23821 gttatcgctg tttgagcata gcgacgttga gattgccgtc gacttcctgc aacgtgcacg
    23881 acaggcgcaa ggcgatatca gcattccaca aagcgcatta attatcggcg gcggtgatgt
    23941 tgcgatggac gtagccagca cgctgaaagt tctcggctgt caggcggtaa cctgcgtagc
    24001 gcgtgaagag ttagatgagt ttccggcaag cgaaaaagag tttgccagcg cccgggagct
    24061 gggcgtttcg atcatcgatg gattcacgcc agtagccgtg gaaggcaata aagtcacgtt
    24121 taagcatgta cggttaccgg gcgaactgac gatggcggca gataaaatta ttctcgccgt
    24181 cggtcagcat gccagactgg atgcctttgc ggagttagag ccgcagcgta acaccatcaa
    24241 aacacaaaat tatcagaccc gcgatccgca agtctttgct gctggcgata ttgttgaggg
    24301 tgacaaaacc gtggtctatg ccgtgaaaac cgggaaagaa gccgccgagg cgattcatca
    24361 ctatttagag ggagcttgct catgttaacg aaagatcttt cgattacttt ttgcggcgtg
    24421 aagtttccca acccgttctg cctctcttct tcgcctgtag gcaactgcta tgagatgtgt
    24481 gccaaagcct acgacacagg ttggggcggc gtggtgttta aaacgatcgg cttttttatc
    24541 gccaacgaag tctcgccgcg ttttgatcat ctggtgaaag aagataccgg ttttatcggc
    24601 ttcaaaaata tggagcagat tgctgaacat ccgttggaag agaatctggc cgccctgcgt
    24661 cagctgaagg aagattaccc ggacaaagta ttgatcgctt cgatcatggg ggaaaatgag
    24721 cagcaatggg aggagctggc gcgcctggtg caagaagctg gcgcggatat gatcgagtgt
    24781 aacttctcct gtccgcaaat gacttctcat gcgatgggta gcgatgtcgg gcaaagcccg
    24841 gagctggtag aaaaatattg tcgggcagtg aaacggggtt ccacgctgcc aatgctggcg
    24901 aagatgacgc cgaatatcgg tgatatgtgc gaagtggcgc tggcggcgaa gcgcggcggc
    24961 gcagatggca ttgcggcgat taacaccgtt aaatccatca ccaatatcga tcttaatcag
    25021 aaaatcggta tgccgatcgt taacggaaaa tcgagtattt ccggatattc cggtaaagcg
    25081 gtaaaaccga tcgccctgcg cttcattcag cagatgcgca cccatccaga actgcgcgat
    25141 ttcccaatca gcggtatcgg cggcattgaa acctgggagg atgcggctga gtttttattg
    25201 ctcggcgcag caacgttaca ggtgaccacc ggcatcatgc agtacgggta tcggatagtg
    25261 gaagatatgg caagcgggtt gtcgcattat ctcgccgatc agggatttga ttcgctgcag
    25321 gagatggtag gtctggcgaa taacaatatt gtcccggcgg aagatttaga ccgcagttat
    25381 attgtctatc cccgtatcaa tcttgataaa tgtgttggct gtggacgctg ttatatttcc
    25441 tgttacgacg gcggtcacca ggcgatggaa tggagcgaga aaacccgcac accgcattgt
    25501 aataccgaga aatgtgtggg ttgtctgctt tgtggtcacg tctgcccggt gggttgtatt
    25561 gagctcgggg aagtgaagtt taagaaaggc gagaaagaac acccggtaac gttgtaaaac
    25621 cagagacgca tccggcattt ggcaccgatt gccggatgca gcgcatccgg caatatccac
    25681 aatcacatca cttcttacgc gcgtatttca gtgaatccag cgctacggcg aagataataa
    25741 tcgccccttt gatgatgtac tgccagtatg ggtttacgcc gatatacgtc aggccatagt
    25801 tgatgacggt aaaaataatt accccggtca ccacgccaat caccgtcccc acaccgccgc
    25861 tgaacgatac accgcctacc acgcacgccg cgatagcatc cagctcatac ataaagccga
    25921 ggttgttggt ggcagagccg atacgtccgg cttctaacat cccgccaaag gcatagaaca
    25981 cgccagacaa cgcgtagatc atcagcaggt tcaggccgac gttgacacca gatacttttg
    26041 ccgcttccgg gttaccgcca atggcaaaaa tgttcttacc gaagcgggtt ttgttccaca
    26101 acacccagac gaacgccacc gcaatcaacg cgtagaaggt gatgtaagag agacggaaac
    26161 tccccagcgc gacaaagccc tgagcaaagg tagagaagcc actgtcaaaa ccagaaattg
    26221 gcgacgcccc gacaaagtcg taatagagcg agttgattcc atagacgatg atcatcgtgc
    26281 cgagcgtggt aatgaacggc gtcacgttga gataagcgat aatcagaccg ttgatcaaac
    26341 cgatcaccgc accaatggca cagacaatca gaataaccag cgcaatcggc atcgtcgcca
    26401 tttccgggaa cactttgttg gcgttatcca tggactgcaa taatgtcgca gccaccactg
    26461 ccgccagccc tacctgacga ccagcagaaa gatcggtccc ctgggtgaca attaaccctg
    26521 ccacaccgag cgcgataata atacgcaccg atgactgggt gagaatatta cttaagttca
    26581 acagacttaa aaatgttggg tcctggaaaa taataatcgc cagcaaaact aaaagaacga
    26641 cgtaaatacc gccctctttc aggtaagtaa gaaaactttt cttatttaac gcactcatgg
    26701 gaagccccta atcttaaagg tgcaaagacg caagacgcag aatttcgttt tgcgttgttg
    26761 tttttgtatc gacaattccg gaaacgagac cattgctcat gaccagaata cggtctgtta
    26821 tccctaacaa ctcaggcatt tcagaggaga taataataat ccccttgcct ttcttcgcca
    26881 gttcggcaat taactgataa atttcaaact tcgccccgac atcaataccg cgcgtcggtt
    26941 catcgagcat taatatttct ggttgcgtta gtagccagcg accaataatc accttttgct
    27001 gattaccacc ggagagcgaa ccaatttgcg tccgatgacc cggcgttttt acccgcatcg
    27061 aatcaatcac ccactgggta tcgcttttca tccgcgagtt atccagtaaa ccaactttat
    27121 ttttgtagtt gcgaatattg gaaattaacg agttaaaact aatatccaga taggcataaa
    27181 ttcccgttga gcggcgctcc tcagttacca gtgcaaatcc gtggtttatg gcttcgttgg
    27241 cattatggtt attgatctgt ttgccgtgca acgtaatggt gccagccgat ttctcgcgaa
    27301 taccaaataa cgtctcaaca atatcggtac gtttcgcccc caccagcccg gcaataccga
    27361 ggatctcccc tttatgcaga tcaaacgaga catcgcgaat cgacggctgg cgcagtgacg
    27421 ttaggttacg cacctcgagg atgacttcgc ccggcttgtt ttctttgtca ggaaaacgct
    27481 gattaagaga acgcccaacc atcatggcga tgatcttgtc catcgtcagt cctgccagcg
    27541 gttcggtggc gatccactga ccgtcgcgca atacggtaac ttcatcacat aactggaaga
    27601 tttcttccat tttatgcgag atataaacaa taccgcagcc gcgctctttt aatttacgaa
    27661 taatagtgaa cagatgattg acctcttttt cggttaacga agaagtcggt tcatccataa
    27721 tcacaatttt cgcgttatag gaaaacgctt tggcgatttc gatcatctgc atttgcgaaa
    27781 cggataatgt gccgacgcgc gcacgcggat cgatatcaat atccagttca tcaaaaatcg
    27841 ctttggtttc gcggtacatt ttgtcctgat cgacaaacat gcctttggtg ggatatcgcc
    27901 ccagccacat gttatccatc accgaacgtt gtaataccag gtttaactcc tggtgtacca
    27961 tcgaaatacc attttccagt gcctctttgg cggaatggaa atcgatctct ttaccctgga
    28021 ataaaatggt gccggagtct ttttgataaa taccaaacag gcattttaat aatgtcgatt
    28081 ttcctgcacc gttttccccc attaatgcat ggatagaatg tggccggact tttaaattaa
    28141 cgttatcaag tgccttaaca ccaggaaagg acttgttgat accgctcatt tccaacaagt
    28201 attccccgga agacggagtc gttgagctga ccatataatt ttaccttgtt ggccatacaa
    28261 taagggcgca gtaaacgact gcgcccaatc agtcttattt cttgctgaat tcagccaggt
    28321 tgtctttatc tacgccaaca taaggtacgc ggaccacttt gttgtcgatt ttccagttgg
    28381 tgccatcagc cgcacctttg ccatcggcca ggtttttcgc cagatcaaag gtcgctttcg
    28441 cctggttgtt agcatcgttc agtactgtgc ccgccagtgc accggatttc accagcgcca
    28501 gcgcttctgg cagcgcatcg acgccaaaca ccggaatgct ggacttgttg tgtgctttca
    28561 gtgcttctac cgcgcccatt gccatcgcat cgttgttggc gataaccact tcgattttgt
    28621 tggcgttcgg gccagacagc caggcgtcca tcttatcttt cgcctgagcg gtatcccaca
    28681 tagcggtatc taactgtaac tgttcagttt taatgccttt gtcgttcagc tctttaatca
    28741 cgtaagtggt acgtgcttct gcatccggat ggcccggttc acctttcagc aatacgaatt
    28801 gaatctgacc gtctttgttc agatcccaac cctgattcgc cgcccagtgt ttagcaatca
    28861 aatcgccctg aataatgccg gactctttgg aatcagtgcc aacgtagtag gctttgtcgt
    28921 agctatccag cgccttacga gacggttctt tgttgaagaa aaccaccggc acgttttgcc
    28981 cacgcgcttt ctcaatcacc gtacccgcag ctgccgggtc aaccaggttg attgccagtg
    29041 ccttcacccc tttcgccagc aatacgtcga tctgatcgtt ctgcttggac tggtcattct
    29101 gagaatcatt catcagcagc tgaacatctg gcgcggcttt cgcatcttgc tcaatagcct
    29161 tgcgcactac agacataaag ttatcgtcgt acttatagat tgttacacca atgcgagtat
    29221 cagcagcgtg tgcagcggca ccgaataaca tgctggccat cacagcagac agggttaaca
    29281 ccttcttatt catggtatct ccggtttttc ttatgcaggg tagtgcttga gataaatgct
    29341 cggcggggca gtagagttaa tgaagtgtta ctgaacgccg aagctcactt tttaaaattc
    29401 gttcttccat gcccggtaac gctccagaaa acggctttaa ttgttgttat gacgctatta
    29461 cctcggtgaa agtgattatt cactgttaca tacgggttac aacgttaaaa cggtgcaatc
    29521 atagctatca cattgttaag atactgtgaa atcactcaca gattgaaagc ggttacatcg
    29581 cctgatttgt tgagttagtg atcgtcgccg cattctggcg tgttgcgaca gaatggcgac
    29641 gcactaacgt cggcataaaa cagtggctag cacgaggatc aatattgcct gctgccccct
    29701 gcaaggccag ttcggtggct aatttcgcca ttgaagcaat gggataacgc acggtcgtta
    29761 attgcgggtc ggtgtaacgg gcgatgggaa tatcatcgaa accgatgatt gagagatgta
    29821 acggaatcgc aatgccatta tcttttaatg ctgtcagtgc gccagcagcc atattgtcgt
    29881 tataagcaaa tacagcggta agttgtagat tgcgccccag cagttcaacc atcgccgcct
    29941 caccgcccgg catgtccggc gtaccagtgc caatccagct ttccggcgga ataatatcct
    30001 gctctttcaa cgcactcatc cagcctgctt tacgcatggc gtcatcttca atgccgtgac
    30061 tggaagaaag ataaccaata cgttgatgac cgttattcag caacatgcgc gtcgccattc
    30121 gggcaccgct gagattatcc aggcaaacgc aacgatgggc gtaccccggc acaacgcggt
    30181 tgattaacac cataccggga atgttatcca taaattgcgc cagttcatcg tcactcaatg
    30241 cttttgagtg aacaatcaac gcattacaac gctggcgaat taacacctca atggcgtgac
    30301 gctctttttc cgcttcatga tagctattgc cgattagcac gtatttctga tgctgctgag
    30361 cgaccagatc caccgctttt accagcgcgc cgaaaaacgc atcagaaacg tccatcacca
    30421 ccacgccaat ggtgtcgcta acctgagttg ccagcgcctg ggcattggcg tttggccgat
    30481 aatccagctc actcacggct ttcattactg cttcacgcgt gtcggcactg accagcgtgc
    30541 tgttattgag cacccgggaa accgttgcca cagagacgcc agcctgacgc gctacatcac
    30601 gaatggtgat catattcact acctgttatg agattgcagt aaatgactgc ttgctggcgg
    30661 ctattttgtc agcactgaat acaggacttc gtgaatcgag tcacagcaat ggaaacggtt
    30721 acagccgttt tgttaacgaa tgtgatccag atcgttatct tgatgtttta gatattgccg
    30781 ttccggcagc acgcaaagtt aactgacgcc agagccattc caccggcccc tggcggaagt
    30841 aacgcagcca gataacagag aagaggatat tcgccagcca taccggaata acaaacgcca
    30901 gcagctccag gcggtcaaaa tgcataaaca aaccgaggtg gtaaaaaagc atggtacaaa
    30961 tcagcgtttg caatagatag ttggtcagcg ccatccgtcc gacgcaggcg atcgcaagca
    31021 ccagcttaaa gcggctcaat tgcggccaga agccataaaa cagcgacgca tagccaatcg
    31081 cctgaaacgg cgcactcagt tcccgcggca tttgaagtaa gaaggcacac cagcgatacg
    31141 cccagtccag ctgccattgc agggcgatgg caggaaggtt aatggtcacc ccaatcgcca
    31201 ccagcacaaa accagtacga cgatagtgac gtaagctgaa ctgccctttc agccagccgc
    31261 tgcgcatcag cgctgcacca atgagcatca tcccagccag ttgccagcca tactgtgcgc
    31321 ccagtgccag taaactgttg ccaacaccat cggcacgatt actgatcgct tcaacgccgc
    31381 cgtgaagctt ccagtatttt tcatacaaaa tagccgatgc atccggcgtc caggcgcggc
    31441 tggtctggct gtcggaaatc agccccaata acagcaaaac gccaagcccc accagataaa
    31501 gcatgacgcc tgtattaaac aggcttttta ccgatggcgc atcgcgcacc agccgccagc
    31561 agattaagcc caccagcccg taagccagca gaatatcgcc gtcccagaac aataaaccgt
    31621 gaataaagcc caataagact aacagcgtta accgcgactg gatccagcgt ctgccacggg
    31681 gcagcaacat ttgcagtcca gcaccaaaca gcagcgcaaa aagcgtgagg aatttcacct
    31741 gggcgatgag atcaagaaat gcccaggtcc atgcatcctg cggcgtaata gcaccgtacc
    31801 aggcgggatt gagataagcc gcctttggta gcccaaaggc gctgatgttt aatagcagga
    31861 tccccagaat ggcgacgccg cgaacaaaat cgagcgtgac gttgcgctcc atggttcctg
    31921 ccttttaatc agttgtgatg acgcacagcg cgcagaaact cgtggcgcgt attctgactg
    31981 gatttgaaca atccaccaag agaggtcgtt gttgtggcac tggttgcatc gcggatgcca
    32041 cgcgccttca cgcagtaatg caccgcgtcg atcgagacag ccacgttatt ggtgcccagc
    32101 agcgtttgta gcgcaataag aatttgctgc gtcagacgtt cctgcacctg cggacgctgg
    32161 gcaaagaact gcacgatacg gttaattttt gacagaccga tcaccgaatc tttcgggata
    32221 taagccaccg tcgctttgcc atcgatggta acaaaatggt gttcacaggt actggtcaga
    32281 gtgatatcgc gcacggtgac catttcatcg accttcattt tgttttcaat gagggtgatt
    32341 ttcgggaagt tggcgtaatc cagaccggag aaaatttcat cgacatacat tttagcgatg
    32401 cgatgcggcg tttccatcaa actgtcatca gccaggtcga gattcagcag ctgcatgatt
    32461 tcggtcatat gaccagcaat aaggcttttg cgcgtttcgt tatccatttc atgcacgggc
    32521 gggcgcagcg gtgtttccag tcctcgcgca actaacgctt catgaaccag ggccgcttct
    32581 ttactgagtg atggcattta tgatttctcc tgcaggtgtg acgcctccgc cctgcgtggg
    32641 ggcaaagtta ttaagctgat ttacagcttg attattgtgc gtgaggcggc gcacataatc
    32701 cagtattcac agcgataatt attgtaattg ccgctgcctt tcatcagcag atgttaaaac
    32761 atcgttatgc aaatacggaa gtgaaagtta ctcacagcac attgaataaa cggtatgatg
    32821 aagaaattgc aaacaacaca acaaggagcc acgcatggaa atgctcgaag agcaccgctg
    32881 ttttgaaggc tggcagcaac gctggcgaca cgactccagt accttaaact gtccgatgac
    32941 gttcagtatc tttctcccac cacctcgtga tcacaccccg ccaccagtgc tgtactggct
    33001 ttccggatta acctgcaatg acgagaactt caccatcaag gcgggtgccc agcgcgtggc
    33061 ggcagaactg gggattgtac tggtgatgcc agacaccagc ccgcgcggcg aacaggttgc
    33121 caacgatgat ggctacgatt taggccaggg cgcggggttt tatcttaacg ccacgcaacc
    33181 gccgtgggcg acgcattacc ggatgtatga ttatctgcgc gatgaattac cggcgctggt
    33241 tcagtcgcaa tttaatgtca gcgaccgctg cgccattagc ggtcattcaa tgggtggtca
    33301 cggtgcgctg attatggcgc tgaaaaatcc gggtaaatac accagcgttt cggcctttgc
    33361 gccaattgtg aatccgtgca gcgtcccgtg gggaatcaaa gcgtttagca gctatttagg
    33421 tgaggacaaa aatgcatggc tggaatggga cagttgcgcg ctgatgtatg ccagtaacgc
    33481 gcaggatgcg atcccgacgc ttatcgatca gggcgataat gatcagtttc ttgccgacca
    33541 gttgcaacct gcggtactgg cagaagccgc gcgccagaaa gcgtggccga tgacgctgcg
    33601 tattcagccg ggatatgatc acagttacta cttcatcgcc tcttttatag aggatcacct
    33661 gcgcttccat gcgcagtatt tactgaagtg aaagtccgcc cggttcgccg ggcatcttct
    33721 catcagaagc gataatccac tgccataaag taacgacgtc cgtcttcgtt atagctgtag
    33781 tcgtcacgac tgagatcctt gtcgccaagg ttcagcacgc ctgcgcgcag tttgacgtct
    33841 ttagtcacct gccaggccgc gccggtattc cagatggtat aaccgcccgg tgttttagcc
    33901 gtcgcgctgt cggcgcgttt ctgcccggta tagtgaccag aaacatagaa tgaccagtct
    33961 tccagcgcca gcggtttcca gtccagcgta ccgttagcag tatggaacgg cagatcggat
    34021 agcggtttgt tttcgccgtt gctgacatca cgaccatcgt tgtaggtgta gttgatcgac
    34081 agtttccatt catcgttgaa cggaattttc agttcggttt ccacgccctg aatacgagct
    34141 ttgttaacgt tgtagtagct aaataccggt atgcgccgtc cgttagcgcc cgtctcaaaa
    34201 ccaacaaagt tttggtagcc cggtgcagcg ttgacgtcag acgtacggct gatgctgata
    34261 cgatctttca catcgttacg gaaaacggta acgctggatt caaccccttc cagccagcct
    34321 tcttcaccca tgtagtaaag ccccagctcc caactttcgc tggtttctgg tttcagatcc
    34381 gggctaccca caatcttaca tgcgccacgg caggaattgc tcgtccagtc agggctaagt
    34441 tgcaacagag aaggtgcttt aaatgccgtc gcccagcccc ctttcaccgt tacggtgtcg
    34501 gtggcgttat aaaccaggta ggcacgcgga ctccagtgtt caccgtaggt ttcgtgatcg
    34561 tccatacgca cgccggtcgt cagcgccagc ggctcgaaga tccgccattc atcttccaca
    34621 aacagcgcgt actggctggc agacgttttg gagctggttc ccccggtcag gttcaccgca
    34681 tcgctaagtt tgtcgtgacg ccattcaccg ccaaccgtga gaaactgatt aattgccgtc
    34741 agcggcaacg tgtatttgcc gtcgaccgta ttgctttcgg aagttatcgg gctgctgttg
    34801 ccagggtttt tgttctcgac tttctcaccg tagtatttca gttcgctggt gccgtaatcc
    34861 caacgcccat tatggctgac ggagtagttc tggcgttcca ggcggttttt gtccagcgag
    34921 tcggaatcac gatcctgacg gtcgaaaccg tatccggcag taaaatcgtg attttgattc
    34981 ggtgtccagg caaattcgac attgccgtcg cggctggaga atccttcaat acgcggcgtt
    35041 tctccggtat cggtggtcgt tgagttttgc gggtcatcct tttcacgttt tgccaggctg
    35101 ccgtaagctt tcattcccag cacaccatca attaatggtc cactggtaaa gaactgaccg
    35161 ttataggtat caccgcgatc gcgatgttcc tgaatggtgg tatcgacggt aacggtaccc
    35221 gaccatttct gaccgatttt tttggtgatg atattcacta caccgccgag cgcatcggag
    35281 ccgtatagcg acgacatcgg gccacgaacc acttcaatac gttcgatgga atcgaccggg
    35341 atccagttca gatcgaaatc attgtggcgg aagacggcat tgcgggagtt aacgcgttta
    35401 ccgtcgacga gaatcagggt gtagctgctg tccagaccac gaatactaac gcccttacgg
    35461 ttatcccctt cgttcgtcag ttgtacgcca ggcacttctt tgaggacatc cttcagattc
    35521 tgtaccggtt ttcgctgcag gtcttcctgg gtaatgacgc tgatactggc gggtgcatct
    35581 ttgaggtttt gttcaacgga agatgcagtg acaaccatcg tttcgccatc atcatcgacc
    35641 gctaacacag gccatgcaca agaaatagcg gacaaacaca gcccgacccg tacgaaaggg
    35701 ttcaacctaa acattccata tctccatgag gtaactacga aaataaaatg ggttatcgct
    35761 cacatcttct tcacgtcccc ttgcgtacgg cagcatcgcg gtggacttat ttgtatgcag
    35821 gtgatcatcc cagaaagccg gaacagcctc tgatttgtca gccttttttg attgcggcgt
    35881 aacgataatg caaacgataa caattatcaa tccaaatgtt aaattttata tccgcatgat
    35941 ttgtcaggga aatggaattg aaaaagccct ctcggttgag agggcttagc aaggaaggga
    36001 ggaaacttat ttcttatcgt tctgcgggaa cttcatttcg ctgtagcgta cgaagtgagt
    36061 tcctttaatc agcttgtagc cgaaccaaat aatcaggaac agcgggatac caatatacgt
    36121 tgccgctacg ccgccccagt caatggtatc tttcaggaac gcttcgtagt tctggcccaa
    36181 agtgataatc agacacagaa tgaatgcgaa gatcggcccc agtgggaaga aacctgaacg
    36241 gtacggcaga tcgttaatgt cgtgtccctg caatacgtaa ccgcgacgga agcgatagtg
    36301 gctaatggca atccccagcc aggcgataaa acccgtcatc ccggaggtgt tcagcagcca
    36361 caggtatacc gtctggttgc caaacatgga ggtcaggaag cacagaccgg caatcaccgt
    36421 cgtcgcatac agcgcattac gcggcacgcc accacgcgac agtttagcga aaatgcgcgg
    36481 cgctttaccg tcacacgcca gggtgtacag catacgagta gacgcataca taccggagtt
    36541 acccgctgac agcaccgccg tcagaataac tgcgttcatc accgccgccg cagagagcag
    36601 acccgcgtgc tggaacacca gggtgaacgg actaacgctg atgtctttaa catcgttacg
    36661 cagcaggctc ggatcggtgt acggaataat caggctgata atcaggatcg cgaacacata
    36721 gaacaacagg attcgccaga acacctgacg taccgcgcgt ggaatgtttt tcgccggatc
    36781 ttcggactcg cctgcagcaa taccgatcag ctcggttccc tggaaagaga agccgacaat
    36841 catagctacg ccgatcatcg ccgcaaaacc accagcaaac ggcgcttcgc cgattgtcca
    36901 gttgctccag cccgcaggct gcgcgccttt gaagataccg ataatcatca gcacgccaac
    36961 gatgataaag acaataactg tcgtgacttt gatcagtgag aaccagtatt ccgcttcacc
    37021 aaagccacga actgagatgt agttcagcag gaagataacg ccgaggaaca acgcactcca
    37081 gatccagccc ggtgtatccg ggaaccacca gctcatgacc agctgagctg caaccaggtc
    37141 aacggcgata gtcaccgccc agttgtacca gtagttccag cccagcgcga agccaaagcc
    37201 ttcttcaaca tagttctgac cgtaagtggc aaacgaaccg gaaaccggca tatatgcagc
    37261 cagttcaccg agactggtca tcaggaagta aaccatcagg ccaatcagca tatacgagag
    37321 caatgccccg cccggacctg cctgagaaat cgttgcgcca gaggcaacaa aaagacctgt
    37381 accgatggaa ccgccaatgg caatcatcgt caggtgacgc gcctttaatt cacggcgtaa
    37441 gcccggcgct tctgtggttt tagtttcgga aaccatacaa aaatgctatc catcttaaaa
    37501 atgaggcgcg attgtagcag acgattggca atccttccgg cagaaataca cggttataag
    37561 acaccttcat gatcgcccag ggattataag taaagcagcc aatctctttt ctggcgaatg
    37621 catgctgagg gcgctgcgtc acgaaatcga cacagcacca gcatgttctt gtacagcaac
    37681 ttaacgcggt acattcgcgg gatcgcaata gtccagaaag cgccgtagcg cgttggaaag
    37741 gtgtttttgc cgatgatgta tacgccacaa cgtacgcatc aggcgcggga gagggaccgc
    37801 aacttcactt aatgtgcctg cctgcaattg atctttaatc acccgtcgcg acaggcaact
    37861 aattcccaac ccatgacgca ccgcatgttt gattgcctcg gagttaccta attccatcgc
    37921 catctcaaac ttcggtaaat gtgacagcaa cagataatcg acaatctccc gcgtgccgga
    37981 accgcgttca cgcaggatcc acggcgcagc ggccagctgt tctaaggtga ccggaccacg
    38041 agccaacggc gaagtcggcg cggcgaaaac caccagctcg tcttccagcc acggttcaga
    38101 aatgatttca gtgctgtggc acggtccttc aataaagcca atatcaacgc ggaaatccag
    38161 caccgcttgc atcacgtcct ggctgttccc aacgctaagt tcaatcggca actgcggata
    38221 atcatggcga taacgggcga taactgcagg cagaatgtag ttaccgatgg tactactggc
    38281 atagatacga atcgcgccgt tgtcttcgcg aaacagttgt tcgatttcaa ccgcctgttc
    38341 aagcaatgcc aacgcacgcg gatagagcag ccgcccgtgt tcattaacaa ccagtctttt
    38401 ccccacgcga tcaaacagtt gcacgccaag ctgcccttcc aggtcggtca aggctgcgct
    38461 cactgctgat tgcgacaacg ccagcatcac cgacgcctgg gtggttgatc cacttttcaa
    38521 tacttctgca aaaacttcca actgccggag ggtgatgtgc atagtcgctt accacttata
    38581 aa
//
LOCUS       DAZFJF010000033        37852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFJF010000033 DAZFJF010000000
VERSION     DAZFJF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.033"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            246..383
                     /locus_tag="ACHKAU_001874"
                     /pseudo
     CDS             246..383
                     /locus_tag="ACHKAU_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(491..1726)
                     /gene="aslB"
                     /locus_tag="ACHKAU_001875"
     CDS             complement(491..1726)
                     /gene="aslB"
                     /locus_tag="ACHKAU_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5530952.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1873..1949)
                     /locus_tag="ACHKAU_001876"
     tRNA            complement(1873..1949)
                     /locus_tag="ACHKAU_001876"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1913..1915),aa:Pro,seq:tgg)
     gene            complement(1992..2078)
                     /locus_tag="ACHKAU_001877"
     tRNA            complement(1992..2078)
                     /locus_tag="ACHKAU_001877"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2042..2044),aa:Leu,seq:cag)
     gene            complement(2099..2174)
                     /locus_tag="ACHKAU_001878"
     tRNA            complement(2099..2174)
                     /locus_tag="ACHKAU_001878"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2139..2141),aa:His,seq:gtg)
     gene            complement(2233..2309)
                     /locus_tag="ACHKAU_001879"
     tRNA            complement(2233..2309)
                     /locus_tag="ACHKAU_001879"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2273..2275),aa:Arg,seq:ccg)
     gene            complement(2412..3797)
                     /gene="yifK"
                     /locus_tag="ACHKAU_001880"
     CDS             complement(2412..3797)
                     /gene="yifK"
                     /locus_tag="ACHKAU_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5530953.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3988..4728)
                     /gene="wecG"
                     /locus_tag="ACHKAU_001881"
     CDS             complement(3988..4728)
                     /gene="wecG"
                     /locus_tag="ACHKAU_001881"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO5530954.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4731..6083)
                     /gene="wzyE"
                     /locus_tag="ACHKAU_001882"
     CDS             complement(4731..6083)
                     /gene="wzyE"
                     /locus_tag="ACHKAU_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO5530955.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6080..7159)
                     /gene="rffT"
                     /locus_tag="ACHKAU_001883"
                     /gene_synonym="wecF"
     CDS             complement(6080..7159)
                     /gene="rffT"
                     /locus_tag="ACHKAU_001883"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO5530956.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(7156..8406)
                     /gene="wzxE"
                     /locus_tag="ACHKAU_001884"
     CDS             complement(7156..8406)
                     /gene="wzxE"
                     /locus_tag="ACHKAU_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO5530957.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8408..9538)
                     /gene="rffA"
                     /locus_tag="ACHKAU_001885"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8408..9538)
                     /gene="rffA"
                     /locus_tag="ACHKAU_001885"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO5530958.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9543..10217)
                     /gene="rffC"
                     /locus_tag="ACHKAU_001886"
                     /gene_synonym="wecD"
     CDS             complement(9543..10217)
                     /gene="rffC"
                     /locus_tag="ACHKAU_001886"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO5530959.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10195..11076)
                     /gene="rfbA"
                     /locus_tag="ACHKAU_001887"
     CDS             complement(10195..11076)
                     /gene="rfbA"
                     /locus_tag="ACHKAU_001887"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5530960.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11095..12162)
                     /gene="rffG"
                     /locus_tag="ACHKAU_001888"
     CDS             complement(11095..12162)
                     /gene="rffG"
                     /locus_tag="ACHKAU_001888"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5530961.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12162..13424)
                     /gene="wecC"
                     /locus_tag="ACHKAU_001889"
     CDS             complement(12162..13424)
                     /gene="wecC"
                     /locus_tag="ACHKAU_001889"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO5530962.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13421..14551)
                     /gene="wecB"
                     /locus_tag="ACHKAU_001890"
     CDS             complement(13421..14551)
                     /gene="wecB"
                     /locus_tag="ACHKAU_001890"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5530963.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14607..15653)
                     /gene="wzzE"
                     /locus_tag="ACHKAU_001891"
     CDS             complement(14607..15653)
                     /gene="wzzE"
                     /locus_tag="ACHKAU_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO5530964.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15665..16768)
                     /gene="wecA"
                     /locus_tag="ACHKAU_001892"
     CDS             complement(15665..16768)
                     /gene="wecA"
                     /locus_tag="ACHKAU_001892"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO5530965.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(17008..18267)
                     /gene="rho"
                     /locus_tag="ACHKAU_001893"
     CDS             complement(17008..18267)
                     /gene="rho"
                     /locus_tag="ACHKAU_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO5530966.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18352..18453)
                     /gene="rhoL"
                     /locus_tag="ACHKAU_001894"
     CDS             complement(18352..18453)
                     /gene="rhoL"
                     /locus_tag="ACHKAU_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO5530967.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18594..18923)
                     /gene="trxA"
                     /locus_tag="ACHKAU_001895"
     CDS             complement(18594..18923)
                     /gene="trxA"
                     /locus_tag="ACHKAU_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO5530968.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19054..20319
                     /gene="rhlB"
                     /locus_tag="ACHKAU_001896"
     CDS             19054..20319
                     /gene="rhlB"
                     /locus_tag="ACHKAU_001896"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO5530969.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20453..21937
                     /gene="gppA"
                     /locus_tag="ACHKAU_001897"
     CDS             20453..21937
                     /gene="gppA"
                     /locus_tag="ACHKAU_001897"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO5530970.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21984..24005)
                     /gene="rep"
                     /locus_tag="ACHKAU_001898"
     CDS             complement(21984..24005)
                     /gene="rep"
                     /locus_tag="ACHKAU_001898"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO5530971.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYSILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24222..24671
                     /locus_tag="ACHKAU_001899"
     CDS             24222..24671
                     /locus_tag="ACHKAU_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO5530972.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24870..25151
                     /gene="ppiC"
                     /locus_tag="ACHKAU_001900"
     CDS             24870..25151
                     /gene="ppiC"
                     /locus_tag="ACHKAU_001900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO5530973.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25238..26713)
                     /gene="ilvC"
                     /locus_tag="ACHKAU_001901"
     CDS             complement(25238..26713)
                     /gene="ilvC"
                     /locus_tag="ACHKAU_001901"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO5530974.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26863..27756
                     /gene="ilvY"
                     /locus_tag="ACHKAU_001902"
     CDS             26863..27756
                     /gene="ilvY"
                     /locus_tag="ACHKAU_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO5530975.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27808..29352)
                     /gene="ilvA"
                     /locus_tag="ACHKAU_001903"
     CDS             complement(27808..29352)
                     /gene="ilvA"
                     /locus_tag="ACHKAU_001903"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO5530976.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29355..31205)
                     /gene="ilvD"
                     /locus_tag="ACHKAU_001904"
     CDS             complement(29355..31205)
                     /gene="ilvD"
                     /locus_tag="ACHKAU_001904"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO5530977.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31270..32199)
                     /gene="ilvE"
                     /locus_tag="ACHKAU_001905"
     CDS             complement(31270..32199)
                     /gene="ilvE"
                     /locus_tag="ACHKAU_001905"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO5530978.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32219..32482)
                     /gene="ilvM"
                     /locus_tag="ACHKAU_001906"
     CDS             complement(32219..32482)
                     /gene="ilvM"
                     /locus_tag="ACHKAU_001906"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO5530979.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32479..34125)
                     /gene="ilvG"
                     /locus_tag="ACHKAU_001907"
     CDS             complement(32479..34125)
                     /gene="ilvG"
                     /locus_tag="ACHKAU_001907"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO5530980.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34128..34178)
                     /gene="ilvX"
                     /locus_tag="ACHKAU_001908"
     CDS             complement(34128..34178)
                     /gene="ilvX"
                     /locus_tag="ACHKAU_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO5530981.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34265..34363)
                     /gene="ilvL"
                     /locus_tag="ACHKAU_001909"
     CDS             complement(34265..34363)
                     /gene="ilvL"
                     /locus_tag="ACHKAU_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO5530982.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
     gene            34716..36236
                     /gene="yifB"
                     /locus_tag="ACHKAU_001910"
     CDS             34716..36236
                     /gene="yifB"
                     /locus_tag="ACHKAU_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO5530983.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36261..36599)
                     /gene="maoP"
                     /locus_tag="ACHKAU_001911"
     CDS             complement(36261..36599)
                     /gene="maoP"
                     /locus_tag="ACHKAU_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO5530984.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36718..37557
                     /gene="hdfR"
                     /locus_tag="ACHKAU_001912"
     CDS             36718..37557
                     /gene="hdfR"
                     /locus_tag="ACHKAU_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO5530985.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37653..37728)
                     /locus_tag="ACHKAU_001913"
     tRNA            complement(37653..37728)
                     /locus_tag="ACHKAU_001913"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37693..37695),aa:Trp,seq:cca)
     gene            complement(37737..37813)
                     /locus_tag="ACHKAU_001914"
     tRNA            complement(37737..37813)
                     /locus_tag="ACHKAU_001914"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37777..37779),aa:Asp,seq:gtc)
BASE COUNT         9274 a        10308 c         9504 g         8766 t
ORIGIN      
        1 aattgccgtt aaaactaaaa aaagcatcaa taatcaacgc gatataataa acctgcctta
       61 catatcaact gcgccagagg taggattgaa aacgctctcc tgattttcca attcattttc
      121 tggataaata aataatttat ttttgtcact attatttatg taatcatcct gtcagggaga
      181 gggatctcaa ttatcaatgc ttaattacgt catcattttg atgacatgaa accggagcat
      241 tttgcatgga attttcgttt tcacccaaac ttcttgttgt tgctgtcgcc gccgctcttc
      301 ctctcatggc cagcgcagca gataccccgt caactgccac cgcacgcaaa ggctttgccg
      361 gatacgatca cccaaaccag tatccacaga acgctgcaat ttattgaatt tgcacgatca
      421 tgtaggccgg ataaggcgtt tacgccgcat ccggcaatca accgcaggcg gccacaaaat
      481 ggccgctttt ttacttactc accaccagca aatgcgcatg cataatgtca ctggccgggc
      541 gaccgtgcgc cagcaaatca gccattgctt taagatatgg cggtagatgg cggaaataac
      601 gctgataccc ggcacacaaa taattcagcc ccggtttgcc gctggcatcg agcatgaagc
      661 ggtgtttcgg gcagcctccc cagcacgctt ttaacacgtt acaactgcga cactgcgccg
      721 gtaattgctt aaatttatct tcaccaaaca cctgctgttg cggggaatcg atcatttctg
      781 caattgtttg ctgatgcata ttccccagcc gatattgcgg ataaacatag tgatcgcagg
      841 cgtaaacgtc gccgttgtgc tcaacaatca ccgagcgccc acaggttggc tgatgatggc
      901 aaaccgcacc cggcgcaccg acaaaattgg caaacgccca ttcgatattc atcacgaaaa
      961 tcttgccgac gtcgcgtttg atccagtggt cgaatatcgc caccagaaat tcaccaaatt
     1021 catcggggcg caccgaccat tccgtcagtt ccccctgaat atcaccaggc gcatgtagtt
     1081 tcagtccatc gctggcagct gtttcatcag ccaggcgctc gaccaccgga ataaactgga
     1141 tgaattcgac tcccgcatcg cacaaaaaat cataaacctg caacggttgc tgcgcgctgg
     1201 tgcggttgac gcagaccagc acgttatagt cgacatgatg tttttgcagc agcgtcaggg
     1261 cacgcatcac cagcttatgc gttggtctgc cacctttggt cacgcgatat tgattgtgga
     1321 tctcagccgg accgtccagc gataacccaa caagaaaatg attttctgcc agaaatgcac
     1381 accatttatc atcgagcagc acgccgttag tctggaagct gttacttatc ttcctgccag
     1441 caccgtattt cgcctgtaac tttacggcac ggcggtaaaa atccagcccg agtaacgttg
     1501 gttcgccgcc ctgccaggta aaagccactt cgttttgcgg ttcgctggca gcgatatagt
     1561 gacggacata cgcttccagc gtgtcatcgt ccatatgcgt gactggcttt tcgcggtaaa
     1621 gggattgttt ttcgagataa aaacagtagt cacagttcag attacaatcg gaaccactcg
     1681 gtttcgccat cacatgaaaa gcacgcgttg gaacctgttg cagcatggtc actccttgcc
     1741 tgaagtcatc cttcaatacg ttaagggcga aatcataccg tggggcaatg agttattacg
     1801 ggcgtttatt gcgaagggag ggtgacgaaa tgcacagaaa acaaaaaacc ccgccgaagc
     1861 ggggttcaaa attggtcggc gagagaggat tcgaacctcc gacccactgg tcccaaacca
     1921 gttgcgctac caagctgcgc tactcgccga aatactgctt tttgaatttt tagttcaatt
     1981 ctttaaagtc gtggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca
     2041 cctgaagcta gcgcgtctac caattccgcc accttcgcat tgtcacaact tctaataatg
     2101 gggtggctaa tgggattcga acccacgaca actggaatca caatccaggg ctctaccaac
     2161 tgagctatag ccaccactac aaatcttgtt acgcggtatt actaccaccg cagctcaagc
     2221 gccgggacta aatggcgcgc ccgacaggat tcgaacctga gacctctgcc tccggagggc
     2281 agcgctctat ccagctgagc tacgggcgct tagcgccgtt gcgggggtgg ataatacgga
     2341 cttcccaccc ctctgtctag tcccttttta aataaattgc gcgtttggtt acggtttgtg
     2401 cgttttgctg attattcctc cagtttatgc gctttcccgt ggcgattaag gccaaaaact
     2461 ttataaatcg ccgtcaccgc cagcataaag atgatgccaa caaacagcga catacgcgta
     2521 tcttcattaa agtacatgcc gatcaaaacg caaatcagga atgccattgt tacgtagttg
     2581 gcccacggga acaggattga gcggaacgga tggctggcaa tcgccgcttt atgtgcacgc
     2641 cgaaaacgca gctggcttat caatatcaca aaccatggca ccatccccgg aagcacgctg
     2701 gcactgtaga cgtagacaaa cacacgctgc ggattgggaa tgatgtagtt caggcatgag
     2761 ccaattagca gaatagcaat agatactgcc acacccgcaa ctggtacgcc gtgacgggaa
     2821 actttcgcca tcgccgccgg taactgacgg tttttcgcca gtgcgtagag catacgtccg
     2881 caactgtaca tgccgctgtt acagccagag agcgcagccg tcagcaccac aaagttgata
     2941 atgcccgccg ctgcggtaat accgattttg gcaaaagtca gtacgaacgg gctgccgttg
     3001 ctgccaattt cattccacgg gaagatggtg acgataacga aaatcgcgcc tacgtagaaa
     3061 atcaggatcc gccacagcac tttgcctacg gcactgcgca gcgtcacctg cggattcttc
     3121 gcttcaccgg cagtaatgcc aatcagctcc acgccctggt aggacgccac cacaatacac
     3181 agcgcggtca ggaacccttt ccagccaccc gcaaagaaac cgccatgctc tgtgagattg
     3241 ctaaaaccaa tcgactgccc gccattgcca aagccaaaga aaatcacgcc caggccaatg
     3301 acaatcatca cgataatcgt ggtgactttg atcatcgcga accagaactc gatttcgccg
     3361 tacaaccgca ccgccgccag attcgccaac gccaccagcg ccactgcgat caatgcgggt
     3421 atccactgcg ccatctccgg gaaccagaac tggacataaa cgccaatggc ggtgatttct
     3481 gagatcccca ccgccatcca cataaaccag taagaccagg cggtgagata accaaagaac
     3541 gggctcatat aacgatgcgc ataaacggcg aacgaaccgg taaccggttc gaggaacaac
     3601 atttcgccca ttgaacgcat gatgaaaaag acgaatagcc cggcgatgat ataggccaac
     3661 aatacggatg gcccggccca tttcaaggta ctggcggccc ccataaacag gccgacgcca
     3721 atggtgcccc ccagggcgat gagttcgata tgtcgagctt ccagcccacg ctgtagctct
     3781 ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat gctttccggt gttaccggtt
     3841 atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa ttcaggagaa atggcaaata
     3901 aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca gacaggcgac ggagtgacca
     3961 ctccgtcgct ttacaaagag aggaaaatca gaggttgccg gtgtagtgcc agcgtaaata
     4021 acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc gaaagcaggc ggtagagcca
     4081 ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt acgtgaccgg tgaaaacatc
     4141 gtaagtcccg ccaacgccca tatacagcgc atctggatgc accagacggc agtcgcgcat
     4201 gaagatctcc tgctttggcg atcccatcgc aacggtgacg atttgcgctc cgctggcatg
     4261 aatgcgttca aacagcgcct gacgctgctc gggtttaaaa taaccatcct gactgccaac
     4321 gatattcaca ttccactggt tgcgcagttt agcttcagtt tgcgccagca cttcaggttt
     4381 accgcccaca agaaataccg gcgtcccttc tttgcctgcg cgcgccatca gctcttccca
     4441 gagatcagca ccggcaacgc gggaaacctg cgcctgcggg tactttttac gtactgaacg
     4501 tacaacgctg atgccatccg catatttaaa ttcggcagcg ttaattagct ccctgacctc
     4561 agcattatct tcaatggtca gcattttttc ggcattaatg gcaaccagcg ttccctgctt
     4621 aagctgcccg tcagcaaaca gataatcgag ggcgtgctgc atatcacgcc aaccaatcaa
     4681 ctgtaagcca cgcagcgtat aggtcggtgc catggtgttg ttattcattg ttatccttca
     4741 acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga gtccggcgct ttcaaaaagc
     4801 cagtacaaca gttttgcgat catcagacat gcgccgaaga ccacgataaa aaagactacg
     4861 cgtgagacaa acgaatccag cccttcacgt gccagcacga tcatattgaa gatcgcccca
     4921 aagcagaaac tgtgcaatat cgcagcctta tagcgattag gctcgcggtt gcccagctca
     4981 tacagccagt cgaaccattt gatgatcaga ccaaccacga tcgccccgag cgggatgaac
     5041 aacgcgccgc ccatcaccac cagtgagcct ataagcgtag gcgagatcgc cagtccggag
     5101 tggttattca gcacttccca ggtaaagtag ttggctgagt tcagcaccat actcgggcga
     5161 cccggccaca gccaggaagg gataaagaca tagaaatcgc ggacaattgg agccaggccc
     5221 tggaagtcga tgttgtcgta gttctgcaac agcaacgcca gattctccca cggcgagaag
     5281 gtgtcgcgag tgagatagag aaacgtatag aacgcttcat cgccgctcac attcattcca
     5341 tagcgtttta gcgccagcca gaacatgccc acaatcccca gcacgcccgc cgccgccagc
     5401 atccacaacg aaatccagcc gcgaataatg ccaataaaca ggaagatagc gaatgcgatg
     5461 atgatattgg cgcgagtgcc gccgacaatc atataagtca gcaagccaaa ggcgaccgtg
     5521 ctgacgagga aaaacagcca cgctttgcta tcctggcgca gaaagtagac caccagcatc
     5581 gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc cggagacttc actggagaag
     5641 atctgactgt aggagttaag ccggaacagc aaaaagccgt tgtgcatgaa gaagatgccg
     5701 acgcttacca gcgcgatgcc catcaggatc acccacgtaa gattggtctc cacgcggttc
     5761 atggtaaaca gcggacggcg cggtacatca gcaacgcgtt tgcgtaggcg ggttttgtag
     5821 gtgacatagt aaaccgcgta gaagcagccc gcagaaagca acgcctgcaa caagatttct
     5881 ggcggcgcga caccaacatc aaagcgaaat accagcacgc tggtcagcgg gaagccgaag
     5941 aaaaaggtga gcaaaaacag caatgaaaag aagacattga agttaaagcg cacacggcga
     6001 aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga gcagccagac cacaaacagg
     6061 ccactgaatt gcagcagact catgcgccct ccccggcggc aatcgccaac gcccgctgcc
     6121 agccttgtag atagttaggg ctaaagaagg caatggtgtt tttatccacc gacgccaact
     6181 ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc ggtagtaaac agcactggca
     6241 aatgctgttc cgtcatatcc tgccagaacg gattttcccg gttaagcaca caaggaatgc
     6301 ccgcctgaat cagtaagcac agcgtaccaa tgccctgctg gcgagcaaaa ataaagtaac
     6361 caagatcgca ctgacgaagt agcgccagat aggcgtcaaa ttccagtttt tcgctcaaaa
     6421 tttgcagatt ttcttcgctg aataactcca gccccgcctg acgaacttcc tcaatgtacg
     6481 cttcgttatt aggcggatat cccatcggca ccaccacttt taccgtatcg ccaaattgct
     6541 gatgaacggc acgtaaagcg gcgacatgct cattgctgag gtcgccggag ttccccacca
     6601 gaatggtcat ttttccttca cgttgccgat cgttcgccat cgtattgaga gaagggtcca
     6661 tccgcgtcgg gaagtacagc agttctcccc gcacctttgg gtgcgttttg gcaaaaaagc
     6721 tcaaatcgcc gcgggtggca aatacacagc cgactcgctt ttgcgccagg cgacgtagtg
     6781 ggtaaaaaag cttatatctc aagccactgg aaagctcata caggtctgcc ccccagatat
     6841 gccagaaaaa ctggctgggc ttaatgccac cactcagcag agccagccac agtgtgggat
     6901 tgaactgacc gtggaaaaaa aatcgctgct gacggttagc ttttgctttc gcgatgaccg
     6961 cttccgccag cgattttttc ccagggaaaa attgcacaga aagcgccgga cagctatcac
     7021 ttaagccgtc gtccttgcca acaaccataa actcgcgcgc gtgctcgctc gtcgcagcca
     7081 gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg agggatatcc gatcccagta
     7141 cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa aacacgccac aacaaagaga
     7201 aaaatagacg atataagttg ccatatatgc ctgcgccgcg cccagtgcac catgcgcagg
     7261 gattagccag tgggcaaata ccatcaataa agtgaactgg ctgatttccg ccagaatata
     7321 aaaccgcaat gacgctttgg cgatcaccag ataaccaaag acataagcgc ccacttttaa
     7381 cacatcaccc actaactgcc aggcaaagag atcgcgcata gcggtaaatt tattcgacaa
     7441 cagcagccag atagcaaaat cacgcagcag ccagacggta aaactcgccg ccgccactgc
     7501 cggtaagacg aatttcagcg atttaaccac ttcccgggtg atatcgcgct tttccgttag
     7561 ccgcgacaac gtgggcagca aatatacgct gaacgatgcc gtaataaatt gcaggtaggc
     7621 atcggaaata ctgctcaccc cttgccagat ccccacttca tcccagctat actgcgccgc
     7681 cagcagttta cgcatcatga tgtaagcaac aggcaaggtc accgacgtaa tcaacgccat
     7741 gagcgtaaat ttgctcaact gccctgccag accgttatcc cagctgggtt tcagatagct
     7801 taacgggatg acaccacgtt tgatcaacat gatagtggca ggaattacca ccagcgcggg
     7861 aatcagcgcc agacccagca acgccccttc ataaccgccc aaacggtaac tgacgtagta
     7921 cgcgagaacg ccaatcaagc tgccgacaat cagcgataac gcattacctg cggcatcgcg
     7981 aaagcctttc atcagcgcca gtaacaggtt gccccaggcg atccccattt gcaccagcgc
     8041 cactaaacgc accagcccct gatagtcggt attaccaaac aatccctggc tgattggcgc
     8101 agctgccagc acaaaaacca gcgccatcag cgtagagaaa ccaagtacca tcgctgatga
     8161 agtgccgacc acgcggcgca gctgttgcgg attatcatgg tactgggcaa cgtatttggt
     8221 tacaccgtta aagatgccag ctccggcaag cacgccgagc acggtaatca actggcggaa
     8281 atttgccgcc agcccaagcc ccgccggacc aaatgacaca gccagcaact taccgaccag
     8341 taacccggca ccaatcttga ccagtgtact ggccgccgtc cacaaggacg cttttgccaa
     8401 cgacatatca ggagaagtag ttcaacaaag tcgcaattac cgtacgctga ttgacgggcg
     8461 acaggttgta gaacagcggc aggcgcagca ggcgctcgct ctctttggtg gtgtagcgat
     8521 cttcaccgtg gaactcacca aagcgttccc ccgcagggca accgtgcagc ggaatgtaat
     8581 ggaacaccgc catgatttcc gcttctttca gaaagttaat caacgcgctc cggtcatcaa
     8641 tatcccgcag tttaatgtag aacatatgcg cgttctgcac gcagccatcg ggaatcgacg
     8701 gcagctcgat acgcccggct ttcgccagag gcgctaacgc atcgtagtag ttttgccaca
     8761 gcgccagacg ttgctggttg atacgatccg ctgcttccag ttgcgcccac aggtacgcag
     8821 cttgcagatc ggacatcaaa tagctggagc caatatcgcg ccaggtatat ttatcgacct
     8881 gaccacggaa gaactggctg cggttagtgc ccttttcacg gatgatctcg gctcgttcga
     8941 ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc acccgccgta tagtttttgg
     9001 tttcatggaa gctaaagcag ccaatatgac caatggttcc cagtgcacgc cctttgtaag
     9061 tggacatcac gccctgagcg gcatcttcta ccacaaacag attatgcttt ttcgccaacg
     9121 ccataatggt gtccatttcg caggccacac ccgcgtaatg gaccggcacg ataacgcgcg
     9181 ttttgtcggt gatcgccgct tcaatcagcg tttcgtcgat gttcatggtg tccgggcgaa
     9241 catccacaaa aacgattttt gcgccacgca gcacaaaggc attggcggtg gagacaaagg
     9301 tgtagctcgg catgatcact tcatcgccag gctggatatc gagcagcagc gccgccatct
     9361 ccagcgaagc ggtgcaggac ggcgtcagta acactttggc gctgccaaaa cgttgctcca
     9421 gccactgctg gcagcgacgg gtaaaaccgc catcgccaca cagtttgccg ctacccattg
     9481 ccgactgcat atagtcgagt tcggttccca ccaccggcgg tgcgttaaat ggaatcatgt
     9541 gatcacctgt ataaccagta cgcggtgctt tctacattcg caccactttg tatgtatcgt
     9601 ttaagcgcgg cggtgttgcc catttgggtc gccacccgca aagttgtttt accgcgagca
     9661 tacgcccagt ttagcgccgt ttgcatcagc tcagcaccta caccgcgtcc agccagcagg
     9721 ccaattcgcg catctgtcgc attgagttcc cgtaaagaga cataaccgcg aataccgccg
     9781 gacgccgcac gtaaaatcag acattgatga tcaaaggtgc cgcgcacggc attttcaatc
     9841 cactgtgcat aaaagcgtct gctggcgtca ggcgcatacc acggcgcacg aaaacggctt
     9901 tgcgcaaatg cggcgctggc taactgacgt aatgcgggaa tatcggtctc ttgtgccact
     9961 acagcaccac tatcactgac attgttcacg ggtagtgcca aatcaacttc accttctacc
    10021 agggagaatc ccagctgttg cagggcatcc agttcacccg tatttgatgc cgcaattttg
    10081 gcctgcaccc gtgaccacgg cgctaacacg tctggcgtca ggagcggtgc ttcagacgta
    10141 atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt cccaggttag tggctcaata
    10201 ctggcgcgga cgggcacgaa gtaactccag cagatattgg ccgtagccag ttttcgctaa
    10261 tgaactggca gcacgcttca caccctcgtc atcgagccag ccgttacgcc aggcgatctc
    10321 ttccaggcag gcaatcttaa agccctggcg tttttctact gtctgcacaa aggtactggc
    10381 ttcaatcagg ctgtcgtgag tgccagtgtc cagccaggca aatccgcgcc cgagcagttc
    10441 aacggtcagg ttgcccgcct cgaggtacat ctggttgatg gaggtaatct ccagttcacc
    10501 acgctccgac ggcttcacct gctttgcgta ctccacgact ttactgtcgt agaaataaag
    10561 cccggtcacc gcccagtttg acttcggctg ttttggcttt tcttccagcg agatagcgcg
    10621 gaaattgtcg tcaaactcca ccacgccaaa gcgttccggg tccatcacct gatagccaaa
    10681 taccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc ttcggactga agccctgacc
    10741 gaagaagatg ttatcgccca gcaccagaca agaaggttca ccgttgagga aggtttcacc
    10801 gatgataaag gcctgcgcca gaccgtccgg gctgggctgt tcggcgtatt ccagctggat
    10861 accgaactca ctaccatcgc ccagcaggcg ctggaaataa cctttatctt ccggcgtagt
    10921 gatgatgaga atttcgcgga taccggccaa catcagcacc gacagcggat agtaaatcat
    10981 tggcttatcg taaatcggca acagttgctt cgatacgccg cgcgtaatcg gatgcaatcg
    11041 ggtgccggaa ccgcccgcca ggataatacc tttcatgttt gcctccgctg aaaattagcc
    11101 tttcagacct aaacgctcgc cctgatagct gccgtcctgc acctgcttcc accagctttc
    11161 attagccaga taccactgca ccgttttacg cattccactt tcaaaggttt cctgtggcag
    11221 ccaaccaagt tcacgggcaa ttttcgaagc atcaatggca taacgcagat catgccccgg
    11281 acggtcagcg acaaaggtga tcaagtcacg atagtgcgcc acgccgtgcg gcttgttcgg
    11341 agccagttct tccagcagct cgcaaatggt ttccacaaca tcgagattct tacgctcgtt
    11401 gtgaccacca atattatagg tttcaccgac tttcccggtg gtcgccacgc aatacagcgc
    11461 gcgggcgtga tcttccacat acagccagtc acggatttgc tgcccgttgc catataccgg
    11521 cagcggttta cccgccagcg cgttgaggat catcagcggg atcagttttt ccggaaagtg
    11581 gtaaggaccg tagttattcg agcagttggt gatcagcgtc ggcagaccgt aggtccgcag
    11641 ccaggcgcgc accagatggt cgctactggc ttttgacgcg gaataagggc tgctcggcgc
    11701 atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc aggtcaccat atacttcgtc
    11761 ggtggagata tgatgaaaac ggaacgctga ttttttatct tccgtcagcg tattccagta
    11821 agcccgcgcc gcttcaagca atgtataagt cccgacaatg ttggtttcaa taaacgctgc
    11881 cgggccgtca atagaacggt caacatggct ttcggctgcc agatgcatga cacagtctgg
    11941 ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg cagatatcaa ctttctcaaa
    12001 ggcaaagtgc tcgctttgcg cgaccggtgc cagcgacatc aggtttccgg cgtaggtcag
    12061 cttatcgacc actaccaccg catcgctcgt ttcgttgatg atataacgca ccagcgccga
    12121 gccaataaac ccggcaccac ctgttatcag aatttttctc atcagcgcca gactcctttg
    12181 gcatcgacga catactgctg atggacattg tcgccattga taactttgaa ctgactatga
    12241 tcgaccagca tcaccagcac gtctgccgtt gccagcgcct cgtcaagctg cgccagagta
    12301 caaagcccgg tcagtttttt cggcaactgg tggatgttag gctcaacaac cagcgtttcg
    12361 ccgctgtgcc actgggcgat cagttcagcg atttccatcg ccgggctttc gcgcaggtca
    12421 tcaatattcg gtttaaacgc cagaccaaag caggcgattt tcagttcact ggcgcgttta
    12481 tcggtagcag caaggcagtc ggcaaccgcc gctttcacct gatcgataac ccagaacggt
    12541 ttgtgatcgt taacttcgcg cgcggtacgg ataagccgcg cctgctgggg gttctgtgcc
    12601 acgataaacc acggatcaac agcaatgcag tgaccgccca cgccagggcc aggctgaaga
    12661 atattaacgc gagggtgacg attcgccagg cgaatcagtt cccagacgtt aatcccctga
    12721 tcggcacaaa tcagcgacaa ttcattagca aaagcgatat tcacatcgcg gaagctgttt
    12781 tcggtgagct tacacatttc cgccgtccgc gagttagtga cgacacactc accttcgagg
    12841 aaaattttgt acagttcgct ggcgcgggtc gaacaaaccg gcgtcatacc accaatcacg
    12901 cgatcgtttt taatcagctc gaccataacc tggcccggta acacgcgttc cgggcagtaa
    12961 gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga aagtgagatc cggacgcatc
    13021 tctgctaacc attctgccat cttctcggtt gaccccaccg gcgaggtgga ttcaaggatc
    13081 accagcgcgc cttttttcag cactggcgca atggagcgag cagccgattc aacgtaggtc
    13141 atatctggct catgatcgcc tttaaacggc gtgggtacag caatcagcca ggcatccgct
    13201 tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta cggcagtttt tactacgctc
    13261 gccaaatcag gctcgacgat atggatttcg ccacgattga tggtatcaac cgcgtgttgg
    13321 ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa acgctgctgc tgtaggcagc
    13381 ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac tcatagtgat atccgattat
    13441 tttttaacgc ttccagaatg cgagagcatg cctgaccatc accatacggg ttatgggcgc
    13501 ggctcatagt ttgatattcg ttttcgtctt ttaaaagacg cgtcacttcc tcgacaattc
    13561 gctgcttatc cgtgcctacc agacgcaccg tacccgccgt caccgcttcc ggacgctcag
    13621 tggtatcgcg catcaccagc accggtttcc ccagcgaagg cgcttcttcc tgaatgccgc
    13681 ctgagtcggt caaaatcagc caggcgtggt tcatcagcca gacaaacggt aaatactcct
    13741 ggggatcgat cagaatgaca tttttcacat gccccagaat gcgattgacc ggctctctga
    13801 cgttcgggtt gagatgcacc ggatagacaa tctggatgtc ctggtgcgtg gtggcgatgt
    13861 ctgccagcgc gtggcagatt tcttcaaagc cacgaccgaa actttcacgc ctgtgaccgg
    13921 tcaccagaat catcttttta tcggggtcga taaacgggta atttgccgcc agttctgaac
    13981 gcagcgtgtc gctgctcatc acctgatcac gcacccataa cagtgcatca atgactgtat
    14041 taccagtaat gaagattcgg ctatccgcaa cgttttcacg cagcaagttt tgccgggaag
    14101 tttcggttgg agagaagtga tacatcgcca gatgcccggt caatgtacgg ttagcctctt
    14161 ccggccacgg cgaatagaga tcgcccgtgc gtaaaccagc ctcaacgtga ccaacaggaa
    14221 tacgctgata aaacgccgcc aggctggttg ccagcgtcgt tgttgtgtcg ccgtgaacca
    14281 gcacgacgtc tggtttgaac tcggcaagaa taggttttag cccttccaga atccgacagg
    14341 ttatctctgt caggccctgt cctggctgca ttatgttgag atcgtagtca ggtacaatgg
    14401 aaaagagttt cagcacctga tcgagcatct cccgatgctg cgcagtgacg caaactttag
    14461 cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg agccatcttg atggcttccg
    14521 ggcgcgtacc aaatacagtc agtactttca catcgattct cttcgaataa gcggcgagcg
    14581 cctttgcgct cgccgcagca gtgttgctat ttcgagcaac ggcgggttaa tgcgacacca
    14641 gccccgatca gccccccgac aatgccccac ataatcatca ggaaggcacg acgtgggcta
    14701 tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat aggtctgaaa acgcggatcc
    14761 agagttggac caacattcag ggtgtttaac atggcccgat tctgatcata gtcgagatca
    14821 aaggccggac cgacggcctg taaattttcc agtcgagcct ggagcattgg acgcccaagc
    14881 aggaacattt ctgaatcagg taattcctcg gcaggcacat ctgtcgcact gcgcgaaata
    14941 ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt tcatccggcg gtcgtagatg
    15001 gctttcgcca cctcttcctg acgcttcacc tgagctttca tctggatggt gcgtgccgcc
    15061 catgcgcctt tcagctcctc attcagatgg ctggctgcac gctggctggc aaaagcaaca
    15121 tactgacgta acaggttatt agcgtcaggc gcggtttcgg caataagctt cacgctgtca
    15181 ttgaccgcgc gggtaaagtc tccggggata aactggatgt tgttaatcat ttcatccagc
    15241 aacgccgcat cggctttgct gttgcccacc atccgctgct tgtaatagtc ggtttgcagc
    15301 cagaactctc tgcgggtatc ccacgaggcc agttgcataa caaactcttt ataggcttcg
    15361 tccatgaccg atggttggtc ggcagaagcc atgtttgaac ggacatccaa gttacgcaaa
    15421 aattgctgct gcgagtaata tccccccagc atattcaccg ttggacgatc ggtaatcgcc
    15481 gtcgcgctcc actcttgtcg agcaaaaaaa gtatacgcca gcgcgattaa cgcaaacgcc
    15541 agccccatgc caataatcca tagcttccca gcccacaagg tacgaaacaa cccacgaata
    15601 tccagttcat tttcagcgtc ttcggccggt ttcccaggca ttggttgtgt catcacatcc
    15661 tcatttattt ggttaaattg gggctgccac cacgatttct acgcagtctg cgttttacgc
    15721 gcttaataaa gcgagcaact ttccaggcac gcttaatgca atatccatag aggaagaatg
    15781 ctagcaaaaa gagcaccagc atgacccact ccgggacaaa atgagaatat tctgccagca
    15841 cgccaatgga agcgagcagt gctgcggcaa gggtaatcag cacaaacgcc tgacgggaag
    15901 taaacccggc acgcatgatc aaatggtgaa tatgctgacg gtcaggagag aatgggctca
    15961 tgcctttacg caggcgacgg tacataatcg ccaccatatc cattagcgga atggcgatta
    16021 tccacaaagc ggtaaccggg ctgatgggat gggttttgcc ctgggtcgtt tcgagcagga
    16081 tccagataac ggtaaaacca atcagcgtac tgcccgcatc acccataaag actttgtagc
    16141 ggcgacccag gataccaagg ttaagcatga tgtatggcag gatggcggcg atcatcgcaa
    16201 agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa aatcataccg attgctgcaa
    16261 acgagacgca ggacaacccg cccagcaagc catcaatgcc atcaaccatg ttgaacgcat
    16321 taatggccgc ccagacggca aatagcgtca ggaagtaacc aaacggtccg agcaccatct
    16381 cccaggagcc aaagatataa cccaggctac tgagataaag tttgccgaac accatcataa
    16441 caatgccaac agcggcctgt atggtggcac ggatttttac gctgatatca aaacggtcat
    16501 ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag atagagagat gcatgcggaa
    16561 tatagtaatc gacaattccg aacgtgaagc aaatccctgc gtaaaccgaa atccccccaa
    16621 cgagaggtat caatccctgg tgacgtttgc ggaagtttgg tttatccact aaaccgactt
    16681 tttttgccac tttacgggca aaaaacagaa acagtgtcgt gaataaaaaa atactgatga
    16741 gatcagtact cactgtcagt aaattcacaa tgcatgctct cagagaaaat tattagcaga
    16801 agtataacca cgaagacctt tattcagaag ggaaagtccg ttccgaaccc accagtcctg
    16861 tgcaaaaatt aaccatttga tatgcaaatt gcataacatg ccagcaaatt ccaggaattt
    16921 tcctaacatc gccagcgcgg cattaagatt acagatataa aagcaaaacg ccacgtaaac
    16981 acgtggcgtt tttggcataa gacaaattta tgagcgtttc atcatttcga agaaatcgtc
    17041 attggtctta gtcatggcca gtttattaat gaggaattcc attgcatcga tttcgcccat
    17101 cgggtgaatg attttgcgca ggatccacat tttctgcagt tcttcctgag tcgtgagcag
    17161 ctcttcttta cgggtaccgg aacggttgta gtcgatagcc gggaagacgc gtttttcagc
    17221 gatcttacga gagaggtgca gttccatgtt gcctgtacct ttaaactctt cgtaaataac
    17281 ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg ataatggtca ggctgccgcc
    17341 ctcttccacg ttacgcgccg caccgaagaa gcgtttcgga cgatgaaggg cattggcgtc
    17401 cacaccaccg gtcaacactt tacctgacgc cggaacaaca gtgttgtaag cgcgcgccag
    17461 acgagtgatg gagtcgagca gaatgataac gtctttcttg tgctcaacca ggcgtttggc
    17521 cttctcgatc accatttccg caacctgaac gtggcgagat gcgggttcgt caaaggtaga
    17581 agcaacaact tcacctttta ccagacgctg catctcggtt acttcttccg gacgttcgtc
    17641 gatcagcaga accatcagca cacaatccgg gtggttgtaa gcaatgctct gagcaatgtt
    17701 ctgcagcagc atagttttac cggctttcgg cggtgccaca atcagaccac gctgaccacg
    17761 accgataggt gatgccagat ccagtacgcg agcggttaaa tcttcagtag aaccgttacc
    17821 acgttccata cgcagacgag agtttgcgtg cagcggggtt aagttctcaa agaggatttt
    17881 gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta actttcagca gcgcaaaata
    17941 gcgttcacct tctttcggcg ggcgaatctt accagagatg gtatcaccag tgcggaggtt
    18001 gaaacggcgg atttggctag gggaaacgta gatgtcatca ggaccggcga ggtaggagct
    18061 gtctgcggaa cggaggaaac caaatccatc ctgcaatatc tccagtacgc catcaccaaa
    18121 gatatcttcg ccactctttg cgtgctgctt caggatggca aaaataatgt cctgcttacg
    18181 catacgagcc aggttttcca gccccatatt ttcgccgaga gtgatcagct cagaaaccgg
    18241 cgtattcttt aattcggtaa gattcatagt ggtgtgagtt cttaaacttg gggtaaatct
    18301 cgaacttaat gttgtgaatg gtatggcagg atcatccatg cctgtttacg gtcatcgact
    18361 catgtctttt cgctgcctgg tcacaggaga gtacgcagaa ctgaaacgac aagacggatt
    18421 gagtgacgag ccagaaatct gttcacttcg catttaagat aaaacgggaa gcgttggaaa
    18481 ttacaagatt caaacttaat aaggtatgtt taatacgaag tcaacactaa gttagcatga
    18541 ctcacgccgg gcgtccagtt tttagcgacg gggcacccga acatgaaatt cccttacgcc
    18601 aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac tttggttgcc
    18661 gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc atatttcggc
    18721 gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc ctgatattcg
    18781 tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca ctctgcccag
    18841 aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact gtcgtcagtc
    18901 aggtgaataa ttttatcgct catatataac tccacaggaa taagcctggc gtgttggttt
    18961 cgttgttggt gtaacattaa ccaactaaag gttgacttta tttcaccgga tacgctttcg
    19021 taaagcaata gtaagctgat attctaccac actatgagca aaacacattt aacagaacag
    19081 aagttttccg acttcgccct gcatccgaag gttgtagaag cccttgaaaa aaaagggttt
    19141 cataactgta cgcccattca ggcactggcc cttccgctga cgctggcggg tcgtgacgta
    19201 gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt ttcttacgtc aacgtttcat
    19261 tatcttctct ctcatcctgc gattgccgat cgcaaggtga atcaaccacg tgccttaatt
    19321 atggcaccga cgcgtgaact tgccgtgcag atccatgccg acgcagaacc gctggcggaa
    19381 gctaccggcc tgaagctggg tctggcttac ggtggtgatg gctacgacaa acagctgaaa
    19441 gtgctggaaa gcggcgttga cattctgatt ggcactacgg ggcgtttaat tgactacgcc
    19501 aagcagaacc acattaacct cggtgccatt caggtggtgg tactggacga agccgatcgc
    19561 atgtacgatc tgggctttat taaagatatc cgctggctgt tccgccgtat gccgcctgca
    19621 aaccagcgcc tcaacatgct gttctccgcc acgctttcgt accgggtacg tgaactggcg
    19681 ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac cggaacagaa aacgggccac
    19741 cgtattaaag aagagctttt ctacccttct aacgaagaaa aaatgcgttt gctgcaaacg
    19801 ctgatcgaag aagagtggcc agacagagcg attattttcg ccaataccaa ataccgttgt
    19861 gaagagatct ggggccacct ggcggcagat ggtcatcgtg tcggtttgtt gacaggcgat
    19921 gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta cccgtggcga tctggatatt
    19981 ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc cggcagtgac gcacgtcttt
    20041 aactacgatt tacccgatga ctgtgaagat tacgttcacc gtattggtcg tacaggtcgc
    20101 gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag agtatgcatt gaatttgcct
    20161 gctattgaga cctatattgg tcactcaatt ccggtaagca aatacaatcc ggacgcattg
    20221 atgaccgatc tgccaaaacc gctgcgcctc acgcgcccac gtacaggcaa tggtccgcgt
    20281 cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa taaaatgtca tactcaaaat
    20341 cattcaaact gtatcaagat ggcaagagaa tgaatcccga tgagcatact gtagtaagtg
    20401 attcaggtga aagaatgcag ccaacacaga gacagattga aggatgaaga gtatgggttc
    20461 cacctcgtcg ctgtatgcag ccattgatct cggttcgaat agttttcata tgctggttgt
    20521 gcgcgaggtg gctggaagca tccagacgct gacgcgaata aaacgcaaag tgcgtctggc
    20581 tgctggcctg aatagcgaaa atgccctgtc taatgaagca atggagcgcg gttggcaatg
    20641 tctgcgcctg tttgctgaac gtctgcaaga tatccctccc tcgcaaattc gcgttgtcgc
    20701 tacggcgaca ttacgtcttg ccgtcaatgc gggtgatttt attgccaaag cgcaggaaat
    20761 cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa gcacgtctca tttatcaggg
    20821 cgttgctcac acaactggcg gtgccgatca gcgcctggtg gtggatatag gcggtgccag
    20881 tactgaactg gtaaccggca cgggtgcaca aaccacctcg ttgttcagcc tgtcgatggg
    20941 ctgcgtcacc tggctggaac gctattttgc cgatcgtaat ctggggcagg aaaattttga
    21001 tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt gccgatgaat tacggtatca
    21061 cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag gcgttacagg aaatcatgat
    21121 ggcacagggg atggatgaac gcattaccct ggaaaagttg cagcaattga aacagcgagc
    21181 cattcattgc ggtcggctgg aggaactgga gattgacggg ctgacgctgg aacgtgcgtt
    21241 agtgttcccg agtggtctgg cgatcctgat cgccattttt accgaactga atattcagtg
    21301 tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc tacggcatgt tgcatctggc
    21361 cgtcgagcag gatattcgca gccgtacgct gcgtaatatt cagcgccgct ttatgatcga
    21421 tattgatcag gcgcagcgcg tagccaaagt tgcggctaac ttcttcgatc aggtggaaaa
    21481 ggaatggcat cttgaagcaa taagccgcga tttgctcatc agcgcctgtc agcttcatga
    21541 aatcggcctg agcgttgact ttaaacaagc gccgcaacac gctgcttatc tggtgcgtaa
    21601 tctggatctt cccggtttta cccccgcaca gaaaaaattg ctggcgacgc tactgctcaa
    21661 ccagactaat ccggtcgatc tctcatcgct gcatcagcaa aatgccgtac ctccgcgcgt
    21721 cgcagaacaa ctctgccgtt tactccgcct ggccatcatt tttgccagcc gtcgccgtga
    21781 cgatctcgtg ccagagatga cattacaggc taaccatgaa ctgttgacct tgacgcttcc
    21841 gcaaggttgg ctaactcaac atccgctggg taaagagatt attgctcagg aaagccagtg
    21901 gcagagctat gtccactggc cgctggaagt gcattaatga gtaagtgccg gatgcgatgc
    21961 tgacgcatct tttccggcct tgattatttc cctcgttttg ccgccatcat cgctttcaga
    22021 ttcgccagat ggctttgccc tttctgcatc cgttcttcgg cgctgaccac tttgcgctcc
    22081 tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa agcggctcgg ctccgggcgc
    22141 accagctcgc cgtactgacg gcgttcttta cacagcgtaa aggtcaattc cttctgggcg
    22201 cgggtaatgc cgacataggc cagccgccgc tcctcatcga tattatcttc atcgatgctg
    22261 ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt agacataagg aaactccagc
    22321 ccttttgacg cgtgcagagt catcagttgc acctgatcca gctcttcttc actctcacca
    22381 cgctccatca tgtcgcgcaa agtaaagcgc gtcaccacct gggtgagcgt catcggctca
    22441 tccagttcgc tgccttccag catctccgtc atccagctaa acagttggtt gacgttcttc
    22501 atgcgcattt cggcggcttt cgggctgggc gatgtttcgt acagccagga ttcataatcc
    22561 atgccgtgga tcagatcgcg caccgcggca atcggctccc gctccgccag acgctggatt
    22621 tctgccaacc agtgagtaaa gcgggtcaat gcttcataac cacgtccgct aagcgtctga
    22681 ctcaggccca tatcaaagct ggcggtaaac atgcttttat tgcgcgtcat cgcccactca
    22741 cccagctttt tcagcgtcgc cgggccaatc tctctcttcg gcgtgttaac gatacgcaga
    22801 aatgcgctgt catcgtccgg gttagtcagc acgcgcagat aagccagcaa gtccttgatt
    22861 tcaggacgag agaaaaacga cgtaccacca gaaattttgt acgggatgcg gttttgcatc
    22921 aggaattttt caaacactcg cgactgatgg ttaccgcgat aaagaataga gtaatctttg
    22981 tactgcgttt tattgacgaa gtgatgcgca atcagttcac cagtgacgcg ctcggcttca
    23041 tgttcttcgt tattcgcgct taaaacttta agctctgtgc cgtaacccag ttcggagaac
    23101 agacgcttct caaagacgtg cgggttattg gcgatcagga tgttcgccgc tttcaaaata
    23161 cgtccggaag agcggtagtt ctgctcaagc ttaatcacct tcagcgccgg aaaatcctga
    23221 ctcagcagca ccaggttttg tggacgtgcg ccgcgccagg agtagatcga ctggtcgtca
    23281 tcgcccacca cggtaaagcg cgcgcggctg cccaccagca gtttcaccag ttcatactgg
    23341 ctggtgttgg tgtcctgata ctcatccacc agcagatagc gaattttgtt ctgccagcgc
    23401 tcgcggactt cttcattgcg ttgcagcagc aacgtcggca ataaaatcag atcatcgaag
    23461 tcgagaacat tgcacgcttt caggtgtgca tcatacagcc cataacaatg ggcaaaaata
    23521 cgatcccgct cgccctttgc ctctgccgcc gcctgcgccg gagttttgag atcgttcttc
    23581 cagttggaga tggtcgaaat cagctgttgc aggagaactt tgtcatcttc aatcagcccc
    23641 tcggtcaact ctttaagcaa agcaagctga tcggtatcgt caaacagcga aaagttcgcc
    23701 ttcatcccaa gcgccgcata ctcgcgtttg atgatatcca accccagcgt gtgaaaagtg
    23761 gagatcatca acccgcgcgc ctctttgcga cccagcgtct gcccgacacg ctctttcatc
    23821 tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat gtcgcgcctg ataaccgcaa
    23881 ccgcggatca gatgggcgat tttattggtg atcacacgag ttttaccgga acccgcgccc
    23941 gccagcacca ggcaggggcc ggtaacgaat tcgacagctt gttgttggcc ggggtttaga
    24001 cgcataggtg tattgctcaa tcttcgaacg gggggaggat tgtagcagaa tcgcatttga
    24061 atgtgaccgc gtgtaagccg ctaaccggtt ggctgcggat gtttaaatca gagagagaag
    24121 accgaaatcg gagttcatcg tgaatccatt agacttagaa aatatcgggt cgtaacgttg
    24181 tgttattact ttttcagagt ttactaagga gaggtttact cgtggcgatt aatttcagcc
    24241 ctaaggttgg tgaaatacag gaatgcaact ttggtaacta tcctgttatc cagaatggac
    24301 agttcagcac cacgtattac gatggccgca ttcctcctga aatgataaaa aacaggttag
    24361 ttgtcgtgct caacggaaaa attaatggca acgcatgtat cgtcgttcca ttatcaacca
    24421 ctcgcgatca tgacaagtta aatcgtggaa tgcatgttga aatagccagt aatgttatca
    24481 atggcctgca attctttgat cagcagattc gttgggccaa agccgacctg gtacaacaag
    24541 tcagtcgaaa ccgattaaat agagcaagga cttacagagg gtatcttaat caatgtttgc
    24601 cacatgagtt ggttgcagac atacagaggg cggtcatcaa gtcaatcaat gccatatcgc
    24661 tcataaattg acaataaaat aaccatcccc gataatgact ttgtagccca tagaggccct
    24721 aagttttctc cccttgaggg acctgcgaag cccggatttt ccgggctttt gccatatatg
    24781 taccagcccc tccctgactg acaattacgt tagcatctgc ggatcatgct aaaatcgccg
    24841 cctgacaatt attcactcaa ggcacgatca tggcaaaaac agcagcagca ctgcatatcc
    24901 ttgtaaaaga agagaaactg gctctggatc ttctcgagca gattaagaac ggtgccgatt
    24961 tcggcaagct ggcgaagaaa cactctattt gcccgtcagg caaacgcggc ggtgatttag
    25021 gtgaattccg ccagggtcag atggttccgg cgttcgataa agtggttttc tcttgtccgg
    25081 tactggagcc gaccggcccg ctgcacaccc agttcggata tcacatcatt aaggtgctgt
    25141 accgcaacta atagcaaggc cttctccagg agaaggcctt gagtgttttc tccctctccc
    25201 tgtgggagag ggtcggggtg agggcatcag cgcgcactta acccgcaaca gcaatacgtt
    25261 tcatatctgt catatagccg cgcagtttct tacctacctg ctcaatcgca tggctgcgaa
    25321 tcgcttcgtt cacatcacgc agttgcgcgt tatctaccgc gccttccgga atagctttac
    25381 ccaagtcgcc cggttgcagc tctgccataa acggtttcag caacggcaca caagcgtaag
    25441 agaacagata gttaccgtac tcagcggtat cagagataac cacgttcatt tcgtacagac
    25501 gcttacgggc gatggtgttg gcaatcagcg gcagctcgtg cagtgattca taatatgcag
    25561 actcttcaat gatgccggaa tcgaccatgg tttcgaacgc cagttcaacg cccgctttta
    25621 ccatcgcgat catcagtacg cctttatcga agtactcctg ctcgccgatt ttgccttcat
    25681 actgcggcgc ggtttcaaac gcggttttgc cggtctcttc acgccaggtc agcagtttct
    25741 tatcatcgtt ggcccagtcc gccatcatac cggaagagaa ttcgccggag atgatgtcgt
    25801 ccatatgttt ctggaacagc ggtgccatga tctctttcag ctgttctgac agcgcataag
    25861 cacgcagttt cgccgggtta gagagacggt ccatcatcag ggtgatgccg ccctgcttca
    25921 gcgcttcggt gatggtttcc caaccgaact gaattagttt ttctgcgtat gccggatcgg
    25981 tgccctcttc caccagcttg tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca
    26041 ggatggtttg ctcacccatc aggtcagatt tcacttccgc aacgaaggac gattccagca
    26101 cgcccgcacg gtgaccaccg gttgctgccg cccatgcttt agcgatcgcc ataccttcgc
    26161 ctttcggatc gttttccggg tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt
    26221 actcttcacg cacttcggtg ccagggcatt tcggcgcaac catgactacg gtgatatctt
    26281 tacggatctg ctcgcccact tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc
    26341 cgtctttcat cagtggctgt acggtgcgca ctacatcaga gtgctgcttg tccggcgtca
    26401 ggttaaccac cagatccgcc tgcgggatca gttcttcgta agtaccaact ttaaaaccgt
    26461 tttcggtcgc tttacgccag gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt
    26521 aggagatatc cagaccagaa tcacgcatgt tcaggccctg gttcagaccc tgtgcgccac
    26581 agccgacgat gactactttt ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc
    26641 gccccataaa gcgacatttg cccagctgtg ccagctgctg acgcagattc agtgtattga
    26701 agtagttagc catggtgatt cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg
    26761 cgagattgaa ttcactatat gacaggaaat ttattgcgga aattgatata ttcacaacgt
    26821 cacattgcaa tttttgcaac gtcaacatcg agggctgcgc cattggattt acgcgatctg
    26881 aaaaccttcc tgcatctggc ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac
    26941 gttagcccat ccacgctctc acggcagatt cagcgcctgg aagaagatct tggtcagccg
    27001 ctatttgtgc gcgataaccg cacggtaacg ctgacggaag cgggcgaaga gctgcgcgtt
    27061 ttcgcccagc aaacgctgtt gcagtatcag caattgcgcc acaccatcga tcagcaaggg
    27121 ccgtcgctct ctggcgaatt acatatcttc tgctcggtga ccgctgccta cagccatctg
    27181 ccgccgattc tggatcgctt ccgcgcggaa cacccgtcgg tggagatcaa actcaccact
    27241 ggtgatgcgg cagatgcaat ggaaaaagtg gtcacgggag aagcggatct ggcgattgcc
    27301 gggaaaccgg agacgctacc cggcgctgtg gcgttttcga tgctggagaa tctggcagta
    27361 gtgctgattg cgcccgcgct gccctgcccg gtgcgtaacc aggtttcggc agagaagccg
    27421 gactggtcta cagtgccgtt tatcatggcc gatcagggac cggtgcgtcg ccgcattgaa
    27481 ctgtggtttc gacgcaataa aatcagcaac ccgatgattt acgccacggt tggcgggcat
    27541 gaagcgatgg tatcgatggt ggcgctcggc tgtggtgtgg cgctgttgcc ggaagtagtg
    27601 ctggaaaaca gccccgaacc ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgaa
    27661 aaaacgccgt ttgaactggg cgtgtgtgca caaaaaaagc ggctgcatga gccgctgatt
    27721 gaggcgttct ggaggatttt gccgaaccac aaatgacgtt gtcgcgcggg taggcctgat
    27781 aagcgaagcg ctatcaggca tttttcccta acccgccaaa aagaacctga acgccgggtt
    27841 attggtttcg tcgtggcaat cgtagcccag ctcattcagc cgggtttcga aatccggttc
    27901 atggtcgcca agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
    27961 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
    28021 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
    28081 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
    28141 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
    28201 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
    28261 gaccgatcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
    28321 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
    28381 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccagccgtt cgccgcgaat
    28441 gttgtgctgg gcgatatatt ttttcattcc cgccagcgcc agcgcgccag agggttccgc
    28501 caccgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
    28561 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcac cgatgcgttt
    28621 taccgctacg ccttcagcaa atagccctac gcgcggcaga tcaaccggat gacccgcatc
    28681 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
    28741 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
    28801 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
    28861 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
    28921 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
    28981 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
    29041 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
    29101 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
    29161 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
    29221 aagacgcgac gacagttttt ccattttttg tagcggcgta acctgcgccg cctcgtaaac
    29281 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccgg acaggggttg
    29341 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
    29401 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
    29461 ggcgtccagg ctttgtcacc tcgagcgtcc tgcgcttcac gacgcgccgc cagttcggca
    29521 tcgcttacct gtaactgaat gccacggttc gggatgtcta tagcgatcag gtcaccatct
    29581 tcaatcaggc caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
    29641 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
    29701 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
    29761 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
    29821 accgcgtcgt cctggctttc gtacactttc gccgggccgg tgaatttgag gatgctgtca
    29881 tcgacgcccg cggttttaac gatgcagccg ttttctgcga aattaccgta gagcaccgcc
    29941 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccatt ggcgcgatcg
    30001 tcatcgagag aatcccaacg gcaatcctgc gagaatgcct gtgtggtacg aatgcctgca
    30061 ggacctgcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgt
    30121 tccagcgttt gcggcaacgt caggccaagt acgtttttca catcacggtt cagtaacccc
    30181 gcgcgatcca gttcgccgag aataccgata acaccaccag cacggtgaac atcttccata
    30241 tggtatttct gggtgctcgg cgcaacttta cacagctgtg gaaccttgcg ggaaagctta
    30301 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
    30361 acggtgttag tcgatccacc catcgcgata tccagcgtca tggcgttttc aaacgccgct
    30421 ttgctagcaa tattgcgcgg cagtgcactt tcgtcgtttt gctcgtagta acgtttggtc
    30481 aattcaacaa tgcgtttacc ggcattgagg aacaactgct tacggtcggc gtgggttgcc
    30541 agcagcgagc cgttgcccgg ctgcgacaga cccagcgctt cggtcaggca gttcattgag
    30601 ttagcggtaa acatcccgga gcaggagccg caggtcggac acgcggaacg ttcaacctga
    30661 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
    30721 agtttgatta tctgatcgga cagtttggtt ttcccggcct ccatcgggcc gccggaaaca
    30781 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
    30841 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
    30901 tcagcgatca gttcgcgaga tggcagtgaa taaagcatcc ccccgtggcc catggcaatc
    30961 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
    31021 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
    31081 gagttcacaa ccgcgataat cggcttgcca aaatcggcgt cggtcattcc ggtggcgcgc
    31141 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
    31201 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat
    31261 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
    31321 gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccacaac ggccttcgcc
    31381 aacctgaata ccatctacac tgcgcactgg cgtgatttct gccgcagtac cggacataaa
    31441 cacttcatcc gccaggtaca gggattcgcg cgacagaacc tgctcacgca cttcaattcc
    31501 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggaggaggt
    31561 gaacggtggg gtgaacagca caccatcttt cacttcaaac aggttttcgc ctgcgccttc
    31621 agagatataa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
    31681 gctacccacc agcagggaag agagatagtt accaccggct tttgccgcgg ttgggatggt
    31741 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
    31801 tgcgcccaga tacgctcccc acgggaaagc ggcgataatc acatcggttg agtatcccgc
    31861 tggcgggttt acacccatgc caacatcacc aacgaagatc agcggacgga tataggcgct
    31921 ggtgagattg tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
    31981 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
    32041 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
    32101 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
    32161 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
    32221 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
    32281 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
    32341 cgatatttat attttgtgca tcgctggcgg cagccatatt cattgagcag acgtggaaac
    32401 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag cgagccgata
    32461 cattgacctg atgttgcatc atgataattt ctccaacatt tctgaattac tggcgccagg
    32521 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
    32581 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
    32641 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggtggt
    32701 ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
    32761 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
    32821 cacattcatc atgaaagagc cgtcaccgga gatacagacg acagtatcgt tcggtcgtgc
    32881 gacttgtgcg ccaaccgccg ctggtaaacc gaaacccatg gtgcctaagc cgctggaggt
    32941 aatgaaattt tccgggcgag tgtgtgcgat gtgctgcgcg gcccacatct ggtgctgccc
    33001 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
    33061 cggcgcgtag atagcgtcac cgggatggtc gtaacgccag gtatgttcat cacgcagctg
    33121 cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaatgctg gtaacagagc
    33181 atttaaatca ccttgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
    33241 gatatccata tggataacac tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg
    33301 gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac tcctgcaccg cgaagttcgc
    33361 cgctttggtg ccatgcattc ccagcatgcc caggtagtac ggataatctg cttcaactgc
    33421 gcccagccct ttcagcgtgc aggtggcagg catttttgtg gtagcgagaa attctcgtaa
    33481 agcaggaact gcctgcgcca tacccacgcc accaccaacg tacagcatcg gtttttgcgc
    33541 ttttgccagc atctggcgcg cttgctcaac ttcggcatgt gggaaagtca cttcgttttc
    33601 aacggtggtg aaccacggtt ccaggtcacc gctggctagc tggatatctt ttgggatatc
    33661 gaccagaacc ggaccaggac gacctgagct ggcaacgtcg aatgcttcag ccataatgcg
    33721 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
    33781 caggacatcc acttcctgaa atgcgtccgt gccgataaac ggtgcggaca cttgaccggt
    33841 gatggcaaca acagggatag aatctaacag tgcgtccgca agcccggtta tcaggttggt
    33901 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
    33961 gatagccgcc attgccgcac cctgctcatg tcggcacagc aagtgctcca cgccgccgtc
    34021 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
    34081 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtc
    34141 ctgaatcttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatattt
    34201 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
    34261 atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
    34321 cgctaatcat gaccaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
    34381 cttgttcgaa ggaatgccta aagagttacc atagattttg cgaatagcac aagatatttt
    34441 ttggccgatt tttatgatgc gtttaataat ttcaatagaa attgttgttt tttaaaaaga
    34501 aaatagtgag tgaaaaaaat tcaatttcag tatgaatgtt gttcccttcc tcgtagttca
    34561 tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
    34621 tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
    34681 atcttatgca cataatagtg actcaaggag ggcttatgtc actgtcaatt gttcataccc
    34741 gcgcagccct gggagtaaat gcgcccccaa tcactgttga ggtacatatc agtaaaggtc
    34801 tacccggctt aacgatggtg ggcttaccag aaacaactgt aaaagaagct cgcgatcgcg
    34861 tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc accatcaacc
    34921 tggctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc gccattgcgt
    34981 tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa ttagtcggag
    35041 aactggcgct tacaggcgct ctgcgtggcg ttcccggcgc aatctccagt gcaactgaag
    35101 ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa gtggggctaa
    35161 ttaacggtga aggatgcctg atagccgatc atctgcaggc tgtctgtgcg tttctggaag
    35221 gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg
    35281 atctcagtga tgttgtcggt caggagcaag gaaagcgagg actggaaatt accgccgctg
    35341 gcgggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
    35401 gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagc gctgcgatat
    35461 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac
    35521 cgcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca gggccaggtg
    35581 aaatttcgtt ggcgcataac ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc
    35641 gtacactgga tgccttgcga gagccgattg aatccgggca gatccatctt tcacgcacac
    35701 gagccaaaat aacctatcca gcccgtttcc agcttgttgc ggcgatgaat cccagcccta
    35761 ccggacatta tcagggaaac cataaccgct gcacgccaga acagacatta cgttatctca
    35821 accggctctc ggggcccttt ctcgaccgct tcgatctctc actggagatc ccattaccac
    35881 cccccggcat tttgagtaaa acggtagtgc cgggagaaag cagcgccacc gttaaacaac
    35941 gcgtaatggc cgccagagag cgccaattta agcggcagaa taaactgaat gcctggctgg
    36001 atagtccgga aatacgccaa ttctgcaaac ttgagagcga agatgcgatg tggttggaag
    36061 gaacactgat ccatctgggg ttatcgattc gtgcctggca gcggttattg aaagttgcac
    36121 gaaccattgc tgatattgat cagtctgaca ttatcacacg tcagcatttg caggaggcag
    36181 ttagctatcg tgcgattgac cgtttgctca tccatctgca aaaactactg acataaaaaa
    36241 agggcatttc gcccttttta ttaatcatca gaatcggtgt agtcttcagc accttcaacc
    36301 tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac gcttaatacg cgtcatatac
    36361 ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt cgccacggca tactgctacg
    36421 aagagtttct cttcctcggt aaccggctcg cgtttgccaa gatccaactc attgaaggca
    36481 taaccatgac gctcaagcag ttgtgcctct ttgatggtga aatcaccatg acgagagaat
    36541 ccacgtggat aatgtttatt gtcgaaatat cgattagtcg tcgtaaagct ttccgccatc
    36601 ctgcacgctc ctaattcttt gaccgagcta gttatggcgc ggagtattag ttacgcttga
    36661 cagagtgtaa aacaaaacat ttaaatcata acgacaaata attttgcgga gagcactgtg
    36721 gatacggaat tgttaaaaac tttcctggaa gttagccgaa cgcgtcactt tggtcgagcg
    36781 gctgaatcgc tctatctgac ccagtcagca gtgagctttc gaatcagaca actggaaaat
    36841 caactgggtg tgaacctttt cacccgccac agaaacaata tccgtttaac cgctgccggt
    36901 gaaaaactac tgccttatgc agaaacgctc atgagcacct ggcaggccgc ccgcaaggag
    36961 gtggcgcata cctcacgaca taacgagttt tctatcggtg ccagcgcctc attgtgggaa
    37021 tgtatgctta atcagtggct gggacgtttg tatcaaaatc aggatgccca tacaggctta
    37081 cagttcgaag cgcgaattgc ccaacggcag tctctggtaa aacagctgca tgaacgccag
    37141 cttgatcttc ttattaccac tgaagcgccc aaaatggacg aatttagtag tcagttgctg
    37201 ggatatttca ctttagcgct ttataccagt gccccttcaa aactaaaggg agatcttaat
    37261 tatctgcgac ttgagtgggg gccagatttt caacagcatg aggcaggttt gatcggtgct
    37321 gacgaagtgc ccattctgac aaccagttct gctgaactgg cacagcaaca gattgcgatg
    37381 cttaatggtt gcacctggct acccgtcagc tgggcgcgta aaaaaggcgg cctgcatacc
    37441 gttgtcgata gcacaacact ttcacggccg ctttatgcca tatggctgca aaatagcgat
    37501 aaaaatacgt tgattcgcga tcttttgaaa attaacgtgc tggatgaagt gtattaatat
    37561 gaatggctgg caaggatgcc ggtagaagga tttacttcgg agagggttat ttcagataaa
    37621 aaaaatcctt agctttcgct aaggatgatt tctggcaggg gcggagagac tcgaactccc
    37681 aacacccggt tttggagacc ggtgctctac caattgaact acgcccctaa ttagggtggc
    37741 ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact
    37801 gaactaccgc tccaccgaat tcttttacaa ccaccggttt tatgaccggc tt
//
LOCUS       DAZFJF010000034        36339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFJF010000034 DAZFJF010000000
VERSION     DAZFJF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.034"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1677
                     /locus_tag="ACHKAU_001915"
     CDS             <1..1677
                     /locus_tag="ACHKAU_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5530986.1"
                     /translation="WPGDVDAGNIDGSIHRWAKNEVIDNDREDWKRISASMRKQPDAL
                     RYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVPE
                     TDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDKALAAG
                     ATEITKEEYLARKNGTYTDLPQNVDTAEDSVQPEPVKVTADEVNKIMQAANISQPDAD
                     KLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQQESEQNDQKTEQNSPNAQQNEP
                     ETKQEPEKACTACGQTGGGSCPDCGAVISDATYQEIFDEENQPEVQENDPEEMEGTAH
                     QHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTSRAGIHLMIDLETMGKNPDAP
                     IISIGAIFFDPQTGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAILTDEIP
                     LDDALLQLREFIDENSGEFFVQVWGNGATFDNVILRRSYERQGIPCPWRYTNDRDVRT
                     MVALGLVMDFDARTTIPFEGERHNALNDARYQAKYVSAIWQKLLPSQADF"
     gene            1745..1987
                     /locus_tag="ACHKAU_001916"
     CDS             1745..1987
                     /locus_tag="ACHKAU_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO5530987.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            1965..2984
                     /locus_tag="ACHKAU_001917"
     CDS             1965..2984
                     /locus_tag="ACHKAU_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5530988.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            3392..4051
                     /gene="yccA"
                     /locus_tag="ACHKAU_001918"
     CDS             3392..4051
                     /gene="yccA"
                     /locus_tag="ACHKAU_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO5530989.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            4142..4471
                     /gene="tusE"
                     /locus_tag="ACHKAU_001919"
     CDS             4142..4471
                     /gene="tusE"
                     /locus_tag="ACHKAU_001919"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO5530990.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(4468..4746)
                     /gene="yccX"
                     /locus_tag="ACHKAU_001920"
     CDS             complement(4468..4746)
                     /gene="yccX"
                     /locus_tag="ACHKAU_001920"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO5530991.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            4841..6031
                     /gene="rlmI"
                     /locus_tag="ACHKAU_001921"
     CDS             4841..6031
                     /gene="rlmI"
                     /locus_tag="ACHKAU_001921"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO5530992.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            6089..6406
                     /gene="hspQ"
                     /locus_tag="ACHKAU_001922"
     CDS             6089..6406
                     /gene="hspQ"
                     /locus_tag="ACHKAU_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO5530993.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6451..6864)
                     /gene="yccU"
                     /locus_tag="ACHKAU_001923"
     CDS             complement(6451..6864)
                     /gene="yccU"
                     /locus_tag="ACHKAU_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO5530994.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            7037..7699
                     /gene="yccT"
                     /locus_tag="ACHKAU_001924"
     CDS             7037..7699
                     /gene="yccT"
                     /locus_tag="ACHKAU_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO5530995.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7795..8253
                     /gene="mgsA"
                     /locus_tag="ACHKAU_001925"
     CDS             7795..8253
                     /gene="mgsA"
                     /locus_tag="ACHKAU_001925"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO5530996.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(8285..10339)
                     /gene="helD"
                     /locus_tag="ACHKAU_001926"
     CDS             complement(8285..10339)
                     /gene="helD"
                     /locus_tag="ACHKAU_001926"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO5530997.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            10462..10908
                     /gene="yccF"
                     /locus_tag="ACHKAU_001927"
     CDS             10462..10908
                     /gene="yccF"
                     /locus_tag="ACHKAU_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO5530998.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            10918..13080
                     /gene="yccS"
                     /locus_tag="ACHKAU_001928"
     CDS             10918..13080
                     /gene="yccS"
                     /locus_tag="ACHKAU_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO5530999.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(13043..13672)
                     /gene="sxy"
                     /locus_tag="ACHKAU_001929"
     CDS             complement(13043..13672)
                     /gene="sxy"
                     /locus_tag="ACHKAU_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO5531000.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            13891..14400
                     /gene="sulA"
                     /locus_tag="ACHKAU_001930"
     CDS             13891..14400
                     /gene="sulA"
                     /locus_tag="ACHKAU_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO5531001.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            14757..15797
                     /gene="ompA"
                     /locus_tag="ACHKAU_001931"
     CDS             14757..15797
                     /gene="ompA"
                     /locus_tag="ACHKAU_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO5531002.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(15872..16324)
                     /gene="matP"
                     /locus_tag="ACHKAU_001932"
     CDS             complement(15872..16324)
                     /gene="matP"
                     /locus_tag="ACHKAU_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO5531003.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVNVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            16510..18270
                     /gene="ycbZ"
                     /locus_tag="ACHKAU_001933"
     CDS             16510..18270
                     /gene="ycbZ"
                     /locus_tag="ACHKAU_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5531004.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            18339..18857
                     /gene="fabA"
                     /locus_tag="ACHKAU_001934"
     CDS             18339..18857
                     /gene="fabA"
                     /locus_tag="ACHKAU_001934"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO5531005.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18927..19094)
                     /gene="rmf"
                     /locus_tag="ACHKAU_001935"
     CDS             complement(18927..19094)
                     /gene="rmf"
                     /locus_tag="ACHKAU_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO5531006.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(19350..19913)
                     /gene="pqiC"
                     /locus_tag="ACHKAU_001936"
     CDS             complement(19350..19913)
                     /gene="pqiC"
                     /locus_tag="ACHKAU_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO5531007.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            complement(19910..21550)
                     /gene="pqiB"
                     /locus_tag="ACHKAU_001937"
     CDS             complement(19910..21550)
                     /gene="pqiB"
                     /locus_tag="ACHKAU_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO5531008.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(21555..22808)
                     /gene="pqiA"
                     /locus_tag="ACHKAU_001938"
     CDS             complement(21555..22808)
                     /gene="pqiA"
                     /locus_tag="ACHKAU_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO5531009.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(22938..24845)
                     /gene="uup"
                     /locus_tag="ACHKAU_001939"
     CDS             complement(22938..24845)
                     /gene="uup"
                     /locus_tag="ACHKAU_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5531010.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(24857..26965)
                     /gene="rlmKL"
                     /locus_tag="ACHKAU_001940"
     CDS             complement(24857..26965)
                     /gene="rlmKL"
                     /locus_tag="ACHKAU_001940"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO5531011.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            27209..28318
                     /gene="ycbX"
                     /locus_tag="ACHKAU_001941"
     CDS             27209..28318
                     /gene="ycbX"
                     /locus_tag="ACHKAU_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO5531012.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(28315..28857)
                     /gene="zapC"
                     /locus_tag="ACHKAU_001942"
     CDS             complement(28315..28857)
                     /gene="zapC"
                     /locus_tag="ACHKAU_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO5531013.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(29031..30041)
                     /gene="pyrD"
                     /locus_tag="ACHKAU_001943"
     CDS             complement(29031..30041)
                     /gene="pyrD"
                     /locus_tag="ACHKAU_001943"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO5531014.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(30152..30889)
                     /gene="ycbF"
                     /locus_tag="ACHKAU_001944"
     CDS             complement(30152..30889)
                     /gene="ycbF"
                     /locus_tag="ACHKAU_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5531015.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(30855..31370)
                     /gene="ycbV"
                     /locus_tag="ACHKAU_001945"
     CDS             complement(30855..31370)
                     /gene="ycbV"
                     /locus_tag="ACHKAU_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5531016.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(31378..31920)
                     /gene="ycbU"
                     /locus_tag="ACHKAU_001946"
     CDS             complement(31378..31920)
                     /gene="ycbU"
                     /locus_tag="ACHKAU_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5531017.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(31932..33002)
                     /gene="elfG"
                     /locus_tag="ACHKAU_001947"
     CDS             complement(31932..33002)
                     /gene="elfG"
                     /locus_tag="ACHKAU_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5531018.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(32993..35593)
                     /gene="elfC"
                     /locus_tag="ACHKAU_001948"
     CDS             complement(32993..35593)
                     /gene="elfC"
                     /locus_tag="ACHKAU_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO5531019.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(35618..36319)
                     /gene="elfD"
                     /locus_tag="ACHKAU_001949"
     CDS             complement(35618..36319)
                     /gene="elfD"
                     /locus_tag="ACHKAU_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5531020.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         8994 a         9406 c         8714 g         9225 t
ORIGIN      
        1 tggcctggtg atgtggatgc cggaaacatt gacggcagca tccatcgctg ggcaaaaaat
       61 gaagttatcg acaacgaccg cgaagactgg aagcgtatct cggcatcaat gcgcaaacag
      121 cctgatgccc ttcgctacga ccgccagact atttttggcc ttgtccgtga acgtccgatc
      181 gacattcaca aagaccctgt ggcactgaac aaatacatta ctgaatacct gactacaaag
      241 ggcgtgtttg aagatgaagg aacaaatcag agcgcaactg atactctctc gtcgccagta
      301 ccagaaactg atgcagtgga aacggcaatt ccggacaacg aaaaaaccga atgcaaagtg
      361 gaagtcgaac catctgtaga gcgtgagggg ccgttctact tcctcttcac cgacaaggat
      421 ggcgaaaaat acggtcgcgc aaacaaactt tctggtctgg ataaggcact ggctgccggg
      481 gctactgaaa tcacgaaaga agaatatctt gcccgcaaaa acggaacata cacggactta
      541 ccgcaaaatg tggataccgc tgaagattcc gtacaaccgg agccggtaaa agttaccgct
      601 gacgaagtaa acaaaattat gcaggcagcc aatatcagcc agcctgacgc cgataaattg
      661 cttgctgcat cacgtggtga atttgttgaa gggattagcg atccgaatga tccgaaatgg
      721 gtgaagggga ttgaaacctg cgattctgtg aaccagaacc agcaagaatc ggaacagaac
      781 gaccagaaaa cggaacaaaa cagcccaaat gcgcaacaaa acgagccaga aacgaaacag
      841 gagccggaaa aagcctgcac cgcctgcggt cagaccggcg gcggtagctg ccctgattgt
      901 ggtgcggtga taagtgacgc aacataccag gaaatattcg atgaagagaa tcagcctgaa
      961 gttcaggaaa atgatccgga ggaaatggaa ggcactgcgc atcagcacaa ggagaacact
     1021 ggcggcaatc agcatcatgc cagcgatagt gaaactggcg aggcgtcaga tcccttaatt
     1081 aaggcgaacg gtcatcataa tctcacatcc accagcagag cggggattca tctgatgatc
     1141 gaccttgaaa ccatgggaaa aaatcctgat gccccgatta tctcaatagg tgcaatattt
     1201 ttcgatccgc aaaccggaga gatggggcca gaattcagca aaactatcga tctggaaact
     1261 gctggcggag tcattgatcg ggacaccatt aagtggtggc tgaaacagtc acgcgaagcg
     1321 caatccgcca ttcttaccga tgaaatcccg ttagatgatg cactgctgca attacgggaa
     1381 tttatcgacg aaaactccgg tgaatttttt gttcaggtct ggggtaacgg tgcaactttc
     1441 gacaacgtga ttttacgccg ttcatatgaa cggcagggga tcccctgccc gtggcgttac
     1501 accaatgatc gcgatgtaag aacgatggtt gctctgggac tggtgatgga tttcgacgca
     1561 agaacgacta ttccattcga aggtgaacgc cataacgctc tgaacgatgc acgttaccag
     1621 gcgaaatacg tttcagccat ctggcaaaaa ctgctcccga gtcaggctga tttttgatgt
     1681 tcaacccata tcgccgccca ccagctatag tggcggcggt catgctgtaa aggcacgtga
     1741 ccacatgtac gaattaactc tatctccagc agagattaaa gagatcacga aatacgagcg
     1801 atacacaaaa cagcaacacc agttaagact gcacggcatc ccatttgtaa tcggccctaa
     1861 aaacgaaccc atcgttctcc gcagggatat tccacacggt ctgacaacga tgccaaaaac
     1921 atctgaactg gtttctgctg aacccgattt tgaggcgctg aacaatggga agaccaagaa
     1981 aaaataaaaa agataatgta ctgccaccgc gggttagatc gaatggttac agttacgtgt
     2041 ggaaacccga aggaagtaca agaagtatag ggctaggaag agtgcggaaa accagcgtag
     2101 ctaaagtctg gcaaaattat gaactggaaa aagcaaaact ccacaacata atgaccgtag
     2161 ctaaattgtg gcacatgttt atggactccc ctgcatttac agaactggcc ccccgaaccc
     2221 aaaaagatta tcgacaacat cagaaggcgt tgctgatggt attcggaaaa gtgcttgctg
     2281 ataatgtcaa aactgagcag gtaagaattt tcatggataa acgagggctt gagagcaaga
     2341 cccaggcaaa tcatgaactg gcaagcctga gtcgagtata cgggtgggga tatgagcgtg
     2401 ggtatgtgaa gaataaccca tgcaaaggag tcagaaaatt ctctcttaaa gcccgcactg
     2461 tttacatcac cgatgaacag tatgcggcga tatatgcgga agcaattcca cagttacgca
     2521 ttgcaatgga gatttcctat ctctgtgcgg caagactcgg tgatgtgctt gagttgaaat
     2581 ggcaggatat tatggataaa gggatctaca ttgagcaaaa caaaaccggc accaaacaaa
     2641 tcaaggaatg gtcaccgcga ttacgtacag cgatccagtt agcccgaaat gtatcttcct
     2701 gtacatgcga atatgtgatc aatacaacca aaggcgggaa agtcatagct aaaacgctga
     2761 ataactggtg gaatcaggct aaacgcgcag ccgagcaaaa agttggcgtt ccgttcgggt
     2821 gtaattttca cgacataaaa gccaagggga tctcagatta cgaaggcagc agtcgcgaca
     2881 aacaaatttt cagcgggcat aaaacagaaa atcaggtgtt gatttacgat cgtaaaacaa
     2941 aaatcacacc aacactggat ttgccgctcg tggttagcaa gtagacaatc cagaaaaatt
     3001 tctggcaaaa agacttggta tactagctat gagcaacttc ctaatacctt gatttttctt
     3061 ggtgttaaat ttgattaact tttcagcaat ttcacgtatt acactgattt ttatttgttt
     3121 ttcttcggtc ttgaaaaccg gcgacccgaa agggttccag agttcgaatc tctgcgcttc
     3181 cgccaaataa gataaggggt tagctaaatg ctaacccctt tttcttttgc ctgtcgaaat
     3241 tctcagggcg ttatatttgc ttaatgacct gataatccgc tgttaaacct gttccagatc
     3301 aaatgcgtaa agatgggtaa aacttctggg tgcccttacg cattatcatt atgctgctta
     3361 attaattaca tctgtcatag agagtgactc aatggatcgt attgttagtt cttcacatga
     3421 ccgtacatca ctgcttagca cccataaggt gctgcgtaat acctattttc tgctgagcct
     3481 gacgctggcc ttttcggcaa ttaccgcaac tgccagtacg gtgctgatgc tgccatctcc
     3541 gggtctgatt ctgacgctgg tgggtatgta tggtttgatg ttcctgacct ataaaacggc
     3601 gaataagccg accgggatta tctccgcatt cgcctttacc ggttttctgg gttatatcct
     3661 cgggcctatt ctgaacacct atctgtctgc cggaatgggt gacgtaatcg ctatggcact
     3721 gggcggaacg gcgttagtgt tcttctgctg ctctgcatat gtgctgacca cccgcaaaga
     3781 tatgtcgttc ctcggcggta tgctgatggc gggtattgtg gtggtgctga ttggtatggt
     3841 tgctaatatc ttcctgcagc tgcctgctct gcatctggcg atcagcgcgg tcttcatttt
     3901 gatctcctct ggcgctattt tgtttgaaac cagcaacatc gttcatggcg gtgaaacgaa
     3961 ctatattcgt gccacggtta gcctgtatgt ttcgctgtac aacatcttcg tcagcctgct
     4021 gagcattctg ggcttcgcca gccgcgatta atctcacccg ctaacacttt catagcctcg
     4081 ctttatgcgg ggctttgttt tttgttacac tgccgccagt ctgattaagg tagtaataat
     4141 tatgctgatc ttcgaaggta aagagataga aacggatacc gaaggctatc tcaaagaaag
     4201 cagccagtgg agtgagccgc tggcagtggt gattgcagag aacgaaggga tttcgctgtc
     4261 accggaacac tgggaagtgg tgcgttttgt gcgtgatttc tatctggaat tcaatacttc
     4321 tccggcgatt cgtatgctgg taaaagcgat ggcgaataaa tttggcgaag aaaaaggtaa
     4381 cagccgctat ttataccgac tgtttccgaa aggtccggct aagcaagcca ccaaaattgc
     4441 tggcctgcca aaaccggtaa aatgtattta acggatacga aaatcggtta attcccccga
     4501 gggatgatgc ggctcgctga gcacccgttc tacacgcgca gaacgcgggc cgccactctt
     4561 taaccacagc attaattttt ccacctgccc ttcttcaccg caggcaacca cttccacgct
     4621 gccatcgtca agatttttgg cgtacccggt taaacccagt cttttcgctt cgtactgtgt
     4681 ggtgtagcga aatcctacgc cctgaactcg cccgtaaacc caggcaatta tgcagacttt
     4741 cgacattgct gctctcctta tccttaatgg acgcgttgca aatccatgga aaactccgga
     4801 caatgacgcc catttctttg catcgacaga aaagtaaatt atgagtgtac gtttagtgtt
     4861 agccaaaggg cgcgaaaaat cattacttcg tcgccatccg tgggtctttt ccggggccgt
     4921 tgcccgcatg gaaggtaaag ccagcctcgg tgaaaccatc gatattgttg atcatcaggg
     4981 aaaatggtta gcacgcggcg cttattcacc agcttcgcaa atccgggcgc gcgtctggac
     5041 gtttgacccg tctgagtcta tcgacattgc ttttttttcc cgccgtttgc aacaagcaca
     5101 aaaatggcgt gactggctgg cgcaaaaaga tggcctcgac agctatcgtt taatcgccgg
     5161 agaatctgat ggcctgccgg gtattactat cgatcgtttc ggtaattttc tggtgctgca
     5221 actgctgagt gctggggcag aatatcagcg cgcggcatta gttagtgccc tgcaaacgct
     5281 gtacccggaa tgtgcgattt acgatcgcag cgatgttgcg gtacgtaaaa aagaagggat
     5341 ggagctgacc cagggcctcg tcaccggcga gttgccgcct gccctgctgc cgattgaaga
     5401 acacggcatg aagctgctgg tggatattca gcacggacac aaaacgggct actacctgga
     5461 ccagcgtgat agccgcctgg ctacccgccg ctacgttgaa aataaacgtg tgctgaactg
     5521 tttctcctat accggtggtt tcgccgtatc ggcactgatg ggcggttgca gccaggttgt
     5581 cagcgttgat acctcccagg aagcgctgga tattgcacgg cagaacgttg agctgaacaa
     5641 actggatctg agcaaggctg agtttgtccg tgatgatgtc tttaaattgc tgcgtactta
     5701 tcgcgatcgc ggtgaaatat ttgacgttat cgtgatggac ccgccgaagt ttgttgagaa
     5761 taaaagccag ttgatgggcg cgtgtcgtgg ctataaagat atcaacatgc tggcgattca
     5821 gttgctgaat gaaggcggta ttctcctgac tttctcctgt tccggtctga tgaccagcga
     5881 tttatttcag aaaatcatcg cggatgccgc aattgatgcc ggccgtgatg tacaatttat
     5941 agagcagttc cgtcaggcag ccgatcatcc ggtgatcgct acctatccgg aagggctata
     6001 tctgaaaggg tttgcctgtc gcgtcatgta acttgaaatg tggaatttta cccttatatc
     6061 aaaggtaaag atttcccggg aggtgactat gattgccagc aaattcggta tcggccagca
     6121 ggtccgccat tccctgttag gttatctcgg agtggtcgtg gatatcgacc cggtttattc
     6181 gctctctgaa ccgtcgcctg atgagctggc ggtgaatgac gagcttcgcg ccgctccgtg
     6241 gtatcacgtg gtgatggagg acgataacgg cctaccggtt catacctacc tggctgaagc
     6301 ccagctaagt agcgaattgc aggatgaaca tcctgaacag ccatccatgg atgagctggc
     6361 gcagaccatc cgcaaacaac tccaggcccc gcgtctgcgt aactgatccc tgaacgaatc
     6421 cccggaaggt ttttgccttc cggggatttt ttatttggcc aggcccaggc gagggatctc
     6481 aatagccggg cagcgatcca tcaccacatt caacccggca tcccgtgcca gtactgccgc
     6541 ttgctcatta atcaccccca gttgcatcca gagggttttc gcgccaatgg caatagcttc
     6601 ctgtgccacg ccccacgccg cttctgaatt acggaaaaca tcgaccatat cgaccttttc
     6661 cggaacgtct gccagcgtgc catacccctg ctggcccagc agcgttttgc cggcaacttt
     6721 tggcgaaacc ggaatgacgt gatacccctg gtcgagtaga tatttcatca cgcgatagct
     6781 gggacgatcg ggtttgtcgc ttgcccccac cagcgcaatg gtgtgagtgg acgttaaaat
     6841 gcctgcaata tcggtttctt tcattatttc ctccagactg tttaacaaag tgtacgacaa
     6901 agccgacatg ccaaccatgc agctaatacg aatgaatgag agcaaaagcg ggaaatatgt
     6961 ctatatgtta gtaagtatga ttaaagaaaa attttgacac attattattc gggcggtctt
     7021 ttgacaggag ctacctatga aaaccggcat cgtgaccacc ttgattgcat tatgtttgcc
     7081 ggtatccgtt tttgccacca cgctgcggct ttcaaccgat gtcgatcttc tggtactcga
     7141 cggcaagaag gtttccagct ctctgctgcg cggtgcggac agcattgaac tggataatgg
     7201 accgcatcag ttagtgtttc gcgttgagaa gacgattcat ctttccaata gtgaagaacg
     7261 gctgtatatc tccccaccgc tggtggtcag ttttaatacc cagctcatca accaggtcaa
     7321 ttttcgcctg cctcgcctgg agaatgagcg ggaagctaac cattttgatg ccgcgccgcg
     7381 ccttgaattg ttggatggcg atgcgacgcc gattccggta aagctggata ttctcgccat
     7441 tacctcaacg gcaaaaacga ttgattatga ggtcgaggtt gaacgctata acaaatccgc
     7501 gaaacgcgct tcactaccgc aatttgccac gatgatggca gatgacagta cgctgctttc
     7561 gggtgtttcc gagctggatg ctattccacc gcagtctcag gtgctcacag aacaacggct
     7621 gaagtattgg tttaaactgg ctgacccaca aacgcgaaat actttcctgc agtgggcgga
     7681 aaaacaacca tcttcctgag atttttgtgc ctgtgcgcag gctttttcgg tctttatctt
     7741 gcagcgataa gtgcttacag taatctgtag gaaagttaac tacggatgta cattatggaa
     7801 ctgacgactc gcactttacc tgcgcggaaa catattgcgc tggtggcaca cgatcactgc
     7861 aaacaaatgc tgatgagctg ggtggaacgg catcaaccgt tactggaaca acacgtactg
     7921 tatgcaacag gcactaccgg taacttgatt tcccgtgcaa ccggcatgaa cgtcaacgcg
     7981 atgttgagtg gcccaatggg gggtgaccag caggttggcg cattgatctc agaagggaaa
     8041 attgatgtat tgattttctt ctgggatcca ctaaatgccg tgccgcacga tcctgacgtg
     8101 aaagccttgc tgcgtctggc gacggtatgg aacattccgg tcgccaccaa cgtggcaacg
     8161 gcagacttca ttatccagtc gccgcatttc aacgacgcgg tcgatattct gatccccgat
     8221 tatcagcgtt atctcgcgga ccgtctgaag taatattgca caggtggcaa acgccacctg
     8281 tttcttacgg ttttctcgcc accggcacat ccagattttt cagtatttcc acaaagggag
     8341 agggattctc tttgttaaac agtgcccata cccgatggcg tgcccgggtc agcgccacgt
     8401 acattaaccg ccgttcttca gcgtccggga aatcctcaac cggtggcagt agcgcctctt
     8461 ccataatcga ctcccgcgcc gcagccggaa aaccatcact cccctcctgc aagccaacga
     8521 tgatgacgta atccgcctgt tgccctttgc tggcatgaat ggtcataaag tcgatttgca
     8581 acttcggcca gcgtgttgcc gctttttcca ggctggcagg cctcatgtga tggtaacgcg
     8641 ccaggataag aatgcgctct tccggtttgg cataaccaga gagcttatcc agcaaagcgt
     8701 caagttgact ctcatccaat aacgtgacgg ctttttcgtc tccattggtt aagctgttta
     8761 gcggcttttt caactggcct gggttctgct gaataaaccg gtttgccacc tcaccgatac
     8821 gactgttaaa acggtaagtc gtgtctaaat cacagcgatc gccttcacca aagttttcat
     8881 ggaaagcggt ggtgagcgac atttgcgcgc cgctgaatcg gtaaatcgcc tgccagtcat
     8941 caccaacggc gaacaacgtc gtctgactgt tttgcttgcg taatgccgct aacaacgctg
     9001 cccgctgcgg cgagatatcc tgaaattcat caaccagaat atgcttccac gggctgataa
     9061 agcgaccttt ctccagaatc acaatcgcct gatgaataag gcccgaaaaa tcgacagcgt
     9121 tttctgcctt cagcgcacct ttccaggctt ttaataacgg ggccatcaac ttgatacgtt
     9181 tactgaacag atcgcgaatc tcttcgggtg cactggcaat catttctgcc tgtgcaccac
     9241 cgtgcatccg catcagactt acccagcgat cgaggcgaga ggcaaggcga cgctgtaatt
     9301 tttcatcatc ccagaagtta ccttctggca ctgaccactg catttcttcc gtcagccatt
     9361 gccgccagcc cttcgcctgc gctttctttt cgctgcattg cttgcgccac tcagcaataa
     9421 agagttcatg acgggcagcg gtatcatttt ccagtttgct gactatcgga acttttttgc
     9481 tgccctgctg aataatatgc agcgccagcg catgaaacgt gcgtgcggta atgtcttcgg
     9541 tatgtagccg ttcgcgaatc cgctcgtcca tctcttcagc ggctttgcga ccaaacgcca
     9601 gcaataaaat ttgctcaggg gacgcttcac cacgcgccag caaccagcct gcacgggcca
     9661 ccagcaccga cgtttttccg cttcctgcac ctgccagcac taacagagaa tgctcgccat
     9721 taacgactgc ccgggcctgc gccggattca gcggtgaaga ctcgacctgg cggaaaaaat
     9781 ccgcatactc ggtaagcatg gcttcggtat acgcctggtt atgctgcaac cgagcgcttt
     9841 caatatcttt caaccaggcc tgacatttac gccacgcctc acggcagtta tcgaattctt
     9901 ccagtcggtt aaccggcaac ggcaacgccg acaaagcctg gcggatttgt tgctgcacgc
     9961 cagaggtttg ctcacgcgtc agccatttat tttccccagt gcgcgtggca atcaaatcca
    10021 gttgctggcg taaaacacca gacgcaattt cgctcatctc gccactccac cgccgccagt
    10081 gagcatcaag atgatggtaa aaacgctgcg tctcgcccca ttcggtgccg tgcagacgca
    10141 ccactttttc gtccggcagt acaaattcca gctcgcccca taccagaccg cgcttacagt
    10201 gaatcgccag taattgattg aaaggaataa ggtattcgtg gcgatcgccg gagactttaa
    10261 tcccggcatt gaggatcacc gcccgatcgt aagggtgctg tgccagacgt tttccaagcg
    10321 ttgtcgcttt cagttccatg actctgtaga tccacacgaa attatttctt atcagtgtaa
    10381 ccgtcagaga aaacgtgctc cagcccaaaa aaacgttaca attgccgccc attactgaaa
    10441 accacagtaa agcgaggttt tatgcgtacc gttttgaaca ttctgaactt tgtgcttggc
    10501 ggatttgcca ccactctggg ctggctgttg gcgactctgg tcagtattgt gctgattttt
    10561 accttaccgc tgacacgatc ctgctgggag atcactaaac tgtctctggt gccttatggc
    10621 aatgaagcta ttcatgtcga tgaactgaac ccggctggca aaaatgtact gctgaatact
    10681 ggcggtacgg tattgaatat tttctggctg attttctttg gctggtggtt atgcctgatg
    10741 cacattgcaa cgggcatcgc acaatgtatt tcaatcattg gcattcctgt cggcattgcg
    10801 aactttaaaa ttgccgctat tgcactatgg ccggttggtc gtcgcgtggt atcggtagaa
    10861 acagcgcaag ctgcgcgtga agccaatgca cgtcgtcgtt ttgaataatc cactaatatg
    10921 gcctttatgc taagtccttt gctcaaacgc tatacctgga acagcgcctg gctgtattac
    10981 gcgcgtattt ttattgcgct ttgtggaacc acagcgtttc cgtggtggct gggtgatgta
    11041 aaactgacga ttccgctaac gctggggatg gtggcagcgg cgctgaccga tctcgatgac
    11101 cgactggcgg gacgtttgcg taacctcatc attacgctgt tctgcttttt tatcgcctcg
    11161 gcctcagtag aattgctgtt tccctggccc tggctatttg cgattggctt aacgctctct
    11221 accagcggct tcattttgct cggcggtctg ggtcaacgct atgcaacaat tgccttcggt
    11281 gcattgctga tcgccattta cactatgttg ggaacatcac tgtatgagca ctggtatcag
    11341 cagccgatgt atctgctggc cggtgccgtc tggtacaacg tcctgacact tattggtcat
    11401 ctgctgttcc cggtccgccc gctgcaggac aacctggcgc gttgctatga acaactggcg
    11461 cgttatcttg agctcaagtc gcgcatgttt gatcctgata ttgaagatga aagccaggca
    11521 ccgctgtacg atttggctct cgccaacggt ctgctgatgg cgacattgaa tcagacgaaa
    11581 ctctcgctgc tgacccgctt acgtggcgat cgtggtcaac ggggaacgcg tcgcacgctg
    11641 cattattact ttgtcgcaca ggatattcac gagcgtgcca gctcttctca tattcagtat
    11701 caaacattgc gtgaacattt tcgccacagc gacgtgctgt tccgttttca gcggctgatg
    11761 tcgatgcagg gccaggcgtg ccagcaactg tcacgctgta ttttgttgcg tcagccttat
    11821 caacatgatc cgcattttga gcgcgctttt acgcatattg atgctgcgct ggagcggatg
    11881 cgcgataacg gcgcacccgc cgatttactc aaaacactgg gatttttgct gaacaattta
    11941 cgcgccattg atgcccaact ggcaacaatt gaatcagaac aggcccaggc actaccccat
    12001 aataatgacg aaaatgagct cgctgatgac agcccgcacg ggttgagtga tatctggctg
    12061 cgtcttagcc gtcacttcac gccggaatcc gccctcttcc gtcatgcggt aagaatgtcg
    12121 ctggtgttgt gcttcggcta cgccatcatt caaataaccg gaatgcatca cgggtattgg
    12181 atcttgctga caagtttgtt tgtctgccag ccaaactata acgccacgcg ccaccgcctg
    12241 aagttaagga ttattggtac gctggtaggt atcgccattg gcattcctgt gctgtggttt
    12301 gtgccatcac tggaagggca gctggtgctg ctggttatta ccggcgtgct cttttttgcc
    12361 ttccgtaacg tgcaatacgc tcatgcaacg atgttcatca cacttttggt gctactgtgt
    12421 tttaacttac tgggtgaagg ttttgaagta gcgttacctc gcgtaatcga tacgctgatt
    12481 ggttgtgcca ttgcgtgggc ggcagtgagc tacatctggc ctgactggca gtttcgcaat
    12541 ctgccgcgca tgctcgaacg cgccacagag gccaactgtc ggtatctcga tgccatactg
    12601 gagcaatacc atcaggggcg tgataaccgt ctggcgtatc gtattgcccg ccgcgatgca
    12661 cacaaccgtg atgctgagct ggcgtcggtg gtatcaaata tgtccagcga gccgaacgtt
    12721 accccgcaaa ttcgcgaagc cgcgtttcgg ttgctgtgcc ttaaccatac gtttaccagc
    12781 tatatctcag ccctcggtgc tcaccgggag cagttaacta atcctgaaat tctggcgttt
    12841 cttgatgacg cagtttgcta tgttgatgac gcgttacatc atcaacctgc tgatgaagaa
    12901 cgcgtcaatg aggcattagc tagcctgaaa cagcggatgc agcaacttga accacgggca
    12961 gacagcaaag aacctctggt cgtacaacaa gttggattat tgattgcatt actgcctgag
    13021 attggtcgtc tgcaacgcca gattactcaa gttccgcagg aaactcctgt ttcggcgtaa
    13081 gagagtcagc ccattctgca agctcctggc ggcgtgccac cgggagcgca gcttcatgaa
    13141 tgccgataat ggcaccttca agcataaaca gaatcttttc tgtcaccaga ctgttttgct
    13201 gccgcagtcg caaccaacac atttttgccc caagtatacg taacgcccgt acatccttaa
    13261 tccccacctc cccgagaatc gcttccagat gaaaagacat attgggcaaa tctttcagtc
    13321 tttcccgggt attgcgcgtg cttttctctt tcagcgctgc atcgagagaa tatttcgaca
    13381 gacgcaccag cttcagttga tttcgccata gactttcatc aacccgatag taattgaggg
    13441 taacggatcg gccacacttt ttatatgtca gccagacagg cggatgtttt acacagtact
    13501 gtgcactttg ctcacaagcc cgaagataca actcaccatc agaaaccatc gcaaacaccg
    13561 tgtcgtcaac ggtcaggctg taactgccaa acaatgatcg gtattcaatt gtgcccaacg
    13621 ttgccaggta ttcttgtgat ttatagatcc gcttatagga gaggcttttc ataaaattcc
    13681 ttttaaaatc ataacataaa agaatgattc acattaacgg atccgttaac tacgaaaata
    13741 ggcaacttat tcttaagggg caagattaat ttatgttttc ccgtcaccaa cgacaaaatt
    13801 tgcgaggctc tttccgaaaa tagggttgat ctttgttgtc actggatgta ctgtacatcc
    13861 atacagtaac tcacaggggc tggattgatt atgtacactt caggctatgc acatcgttct
    13921 tcgtcgttct catccgcagc aagtaaaatt gcgcgtgtct ctacggaaaa cactacagcc
    13981 gggcttatca gtgaagttgt ctatcgcgaa gatcagccca tgatgacgca acttctactg
    14041 ttgccattgt tacagcaact cggtcagcaa tcgcgctggc aactctggtt aacaccgcaa
    14101 caaaaactga gtcgggaatg ggttcaggca tctgggctac ccttaacgaa agtaatgcag
    14161 attagccagc tctccccttg ccacactgtg gagtcaatgg ttcgcgcttt acgcacgggc
    14221 aattacagtg tggtgatcgg ttggttggca gatgatttga ctgaagaaga gcatgctgaa
    14281 cttgttgatg cggcaaatga aggtaacgct atggggttta ttatgcgtcc ggtaagcgca
    14341 tcctctcacg ccacgagaca actttccggg ctaaaaattc actctaattt gtatcattaa
    14401 gtaaatttag gattaatcct ggaacttttt ttgtcgccca gccaatgctt tcagtcgtga
    14461 ctaattttcc ttgcggaggc ttgtctgaag cggtttccgc gattttcttc tgtaaattgt
    14521 cgctgacaaa aaagattaaa cataccttat acaagacttt tttttcatat gcctgacgga
    14581 gttcacactt gtaagttttc aactacgttg tagactttac atcgccaggg gtgctcggca
    14641 taagccgaag atatcggtag agttaatatt gagcagatcc cccggtgaag gatttaaccg
    14701 tgttatctcg ttggagatat tcatggcgta ttttggatga taacgaggcg caaaaaatga
    14761 aaaagacagc tatcgcgatt gcagtggcac tggctggttt cgctaccgta gcgcaggccg
    14821 ctccgaaaga taacacctgg tacactggtg ctaaactggg ctggtcccag taccatgaca
    14881 ctggtttcat caacaacaat ggcccgaccc atgaaaacca actgggcgct ggtgcttttg
    14941 gtggttacca ggttaacccg tatgttggct ttgaaatggg ttacgactgg ttaggtcgta
    15001 tgccgtacaa aggcagcgtt gaaaacggtg catacaaagc tcagggcgtt caactgaccg
    15061 ctaaactggg ttacccaatc actgacgacc tggacatcta cactcgtctg ggtggtatgg
    15121 tatggcgtgc agacactaaa tccaacgttt atggtaaaaa ccacgacacc ggcgtttctc
    15181 cggtcttcgc tggcggtgtt gagtacgcga tcactcctga aatcgctacc cgtctggaat
    15241 accagtggac caacaacatc ggtgacgcac acaccatcgg cactcgtccg gacaacggca
    15301 tgctgagcct gggtgtttcc taccgtttcg gtcagggcga agcagctcca gtagttgctc
    15361 cggctccagc tccggcaccg gaagtacaga ccaagcactt cactctgaag tctgacgttc
    15421 tgttcaactt caacaaagca accctgaaac cggaaggtca ggctgctctg gatcagctgt
    15481 acagccagct gagcaacctg gatccgaaag acggttccgt agttgttctg ggttacaccg
    15541 accgcatcgg ttctgacgct tacaaccagg gtctgtccga gcgccgtgct cagtctgttg
    15601 ttgattacct gatctccaaa ggtatcccgg cagacaagat ctccgcacgt ggtatgggcg
    15661 aatccaaccc ggttactggc aacacctgtg acaacgtgaa acagcgtgct gcactgatcg
    15721 actgcctggc tccggatcgt cgcgtagaga tcgaagttaa aggtatcaaa gacgttgtaa
    15781 ctcagccgca agcttaagtt ctcgtctggt agaaaaaccc cgctgctgcg gggttttttt
    15841 gcctttagta aattgaactg actttcgtca gttattcctt acccagcaat gcctgcagat
    15901 cctgcttcag agaagacatt ttattcgcgt atttctcttt gttttccgca tcttcaatca
    15961 gctgaacaat cgtttcagaa agcgttttac cgcgacgctg cgcaagacca gccagacgtt
    16021 gccagacaat aaattccaga tcgatcgatt ttttgcgcgt atgctggtgt tccgcattaa
    16081 aatggcgctt tcgtcttgcc cgaatggttt gcttcatgcg attgaccagt tccggattca
    16141 tatgcttgtc aatccagcca tttaccagca cgggttcatt ttcgagcgag agcaacacat
    16201 tgacggcttc ctgggcggca ctggcttcta tgtaacgggt gattaactcc ccttcgcgat
    16261 gcttcttcac cagatacttc catttccaac cgctttcaag attttcaagt tgttgatatt
    16321 tcatagcgat ctcaatgtta ccgtgtaact ctttacagaa tatcagcttt ttaggccagt
    16381 gaagaaaaga atctccatcc tgtgagcttt aacgcccatg ccaggagtat tgtcggtgct
    16441 ttctacgtgt gctgtagtgc cggttacggt ataatcgcgg ctttgacaac agactaaaaa
    16501 acatcaactt tgaccattac gaaacttgca tggcgtgacc tggttcctga taccgatagc
    16561 tatcaggaaa tatttgctca gccacatttg attgacgaaa acgatccttt attcagtgat
    16621 actcaaccgc gactgcaatt cgcactggag cagttgctgc atacgcgagc atcctcctct
    16681 tttatgctgg cgaaggcccc ggaagagtct gagtatctga atcttattgc caatgccgcg
    16741 cgtacgctac aaagcgatgc aggccaactg gtgggcggtc actatgaggt ttccggccac
    16801 tccatccgct tacgtcacgc agtgagtgca gatgataatt ttgcgacttt aacgcaagtt
    16861 gtcgctgccg actgggtaga agcggagcaa ctctttggct gcctgcgcca gtttaatggc
    16921 gacattaccc tgcagcctgg tctggtgcat caggcaaatg gcggtattct catcatctct
    16981 ttgcgtacac tgctggcgca acctctgctg tggatgcggc tgaaaaatat cgttaaccgc
    17041 gagcgttttg actgggttgc gtttgatgag tcgcgccctc tccccgtctc tgtgccttcg
    17101 atgccattga agctgaaagt cattctggta ggcgaacgcg aatcattgtc tgatttccag
    17161 gagatggagc cagagctttc agagcaggct atttatagcg aatttgaaga tactctgcag
    17221 attgtcgatg cagagtcagt aacccagtgg tgtcgctggg tgacatttac cgccagacat
    17281 aatcacttac ctgcaccggg agcggatgcc tggccggtac ttatccgcga agcagcacgc
    17341 tacaccggtg aacaagaaac acttccgctt agcccgcagt ggatcctccg ccagtgtaaa
    17401 gaggtcgcct ccctgtgtga tggcgacacc ttctccggcg agcagctaaa cttaatgctg
    17461 cagcagcgtg aatggcgcga aggtttcctc gctgaacgta tgcaggatga gatccttcag
    17521 gagcaaatcc tgattgaaac cgaaggcgaa cgcatcgggc aaattaacgc cctttcggtc
    17581 attgaatttc cgggtcatcc acgcgctttt ggcgaacctt ctcgcattag ctgcgttgtg
    17641 catattggcg atggtgaatt caccgacatc gaacgcaaag cggagcttgg cggcaatatc
    17701 catgcgaaag ggatgatgat catgcaagcg ttcctgatgt cggaactaca gcttgagcaa
    17761 cagatcccct tctcagcatc gctgacattt gagcagtcat acagtgaagt tgatggagat
    17821 agtgcctcga tggctgaact ctgcgccctg ataagcgccc tcgccgatgt gccggtgaat
    17881 cagagtatcg ctatcacagg ttcagtcgat cagttcggtc gcgcccagcc ggtcggtggt
    17941 ttaaatgaga aaatcgaagg cttctttgct atttgccagc aacgtgagtt aaccgggaaa
    18001 caaggtgtca ttatccccac agctaacgtt cgccatttaa gtcttcacag tgaactggtg
    18061 aaagcggtag aagaaggcaa attcaccatc tgggcagtag acgatgtgac tgacgcactg
    18121 ccgttattat taaatctggt gtgggatggc gaaggccaaa cgacgctgat gcaaaccatc
    18181 caggaacgta tcgcgcaagc atcgcaacag gaaggacgtc accgttttcc atggccatta
    18241 cgttggctga actggtttat tccgaactga tcggacttgt tcagcgtaca cgtgttagct
    18301 atcctgcgtg cttcaataaa ataaggctta cagagaacat ggtagataaa cgcgaatcct
    18361 atacaaaaga agaccttctt gcctctggtc gcggtgaact gtttggcgct aaaggcccgc
    18421 aattgccagc accgaacatg ctgatgatgg accgtgtggt caaaatgacc gaaacgggtg
    18481 gtaacttcga caaagggtat gttgaagcag aactggatat caatccggat ctgtggttct
    18541 tcggatgcca ctttattggc gatccggtta tgccgggatg cctgggcctg gacgcaatgt
    18601 ggcagctggt agggttctac ctcggctggc tgggcggcga aggtaaaggc cgcgcgctgg
    18661 gcgttggcga agtgaaattc actggtcagg tactgccgac agcgaaaaaa gtgacctacc
    18721 gtattcactt taaacgcatt gttaaccgtc gtctgattat gggcctggcg gatggcgaag
    18781 tgctggttga tggtcgtctg atctataccg ccagcgacct gaaagtcggt ctgttccagg
    18841 atacgtctgc cttctgatta taatcagaca gagtatcaaa aggcgaaacc tccgcaatgc
    18901 ggaggtttct ttttaaagag acagaatcag gccattacta ccctgtccgc catggcttct
    18961 cgccagcctc ccagccattg tgacctttga ttcagcgtct gatagggaca catttctttt
    19021 gagcgtccgg cgatgccggc ctgataacca cgttgatgtg cccgttccag gcgatctcgt
    19081 ttttgtctct tcatgcctcg tttccctcat actggtttct ggtggaaaag aaaacagtga
    19141 ttactaaatg tgcaatcaca gttaagcaat accgcgaatc ataactgccg tcaatgcgca
    19201 aaattcacgc cactgtcata tttgtgagct acacgggtat gccggttgta caaaaaatgt
    19261 gaagaggaac aactttttcc gcacgttaga gacaaaaaaa ccgcctcagg caacataccg
    19321 gaggcggtta tatttacaaa tctttataat taaggtagac gctttatctc ttgcgcaata
    19381 gaagcggctt cctgactcca ggcactggcc agtactttaa ccatctcatc gtaaccatcc
    19441 tgagtttgca ccccttccag acggaacgga cgtttgatca gttgtccctg gtggttcaac
    19501 agccactcac cactgacaat gaccttgcca tcatagcgac cgttaaactc cgtgacggta
    19561 acattgagcg tgtcctgggc gcttcccaga ggctgggagg caaccaccca gccgggcagt
    19621 tgcgtactca ggttggcaac cagggtgttg cgcaactgtt gatccaacgg gctggcccac
    19681 aagttgttat tggcaatcac atacttcaca tcactggttt gataaaccac accattcccc
    19741 gccagatagt caggaacagt gacctgctct acccataaca gacgattgcc ctggctggcg
    19801 gtactttgtg taccgctctg caccacaggt aactggtaat agtttttatt aatttcgccg
    19861 gagctgcatc cggccagcca cagtgctgca atcgtcacta gccacttttt cattgtttcg
    19921 ccctcttcgg ctctggatct tttttgtcct tcgcttcaaa taccagcgcg ttactcttct
    19981 cattgagcgt tttcagcacc ggttgcagtt ctcgcaacac ctgatcaagg cgctgcatat
    20041 ccgccaccat cttgttgtag gctgcggagc caggctggaa gccctgcatg ctgcgattca
    20101 attcacgcaa cgttgactgc atatccgtcg gcaactgctg catcgactgg ctagcgagga
    20161 tcttgttcat gctatccagc gtcgtttgca ggtttttcat tgtgcgctga ctttcagaaa
    20221 gcgtactggt tgcctgttca atcatcggat tcaatggcag tttgttgatc ttatccaacg
    20281 cttccatcag tcgttgctgg atttgcgcca ggccgccgct aacggtcggg ataatctgat
    20341 aaccattaaa ttcacgaata ccggttattg caggcgtatt tggatagaaa tcgagatcaa
    20401 catacagtgc accagtgacc aggtttccgg ttttcagcga tccgcgtaaa ccacgtttca
    20461 acaattcgcc aaggtgctca acaacatccg cattttcgcc aagctgcatt ttcagccgct
    20521 ctggctcgat acgaatcagt accggaatac ggtaatcatc gttaaatgtc tgacgcatat
    20581 tcggcgcaaa gaatggcact ttgcttacgg tacccaggcg aataccccgg aactctaccg
    20641 gagctcccgg ttgcagaccg cgtaccgaat ctttaaagaa catcagataa tcaatgtgat
    20701 cggtgtacaa cgaatcctga atgctcttct gatcatcata caaaacgaaa gctgttttcg
    20761 gtgccactgg ctgccctaaa tccagacctt ccggcacatc aaagctgaca ccgccactca
    20821 gcagcgttgt caatgagccc atctccacac gcatccctgc tgacgtcaga tcaaccgcaa
    20881 tgccactatc tttccagaag cgaacattgt tggtcaccag tcggtcataa ggtgcattga
    20941 tgaacagttg atagctgata ttgcgttttt gtgtatcgaa ggtgctggtt tcaaccgaac
    21001 ctacccgata gccacggaac agcaccggat ctcctggcga gagctgcccg gcttttttgc
    21061 tatcgagaat cacacggata cctttcgcat caggcggggc caacggtggc gagtccagca
    21121 aatcgtattt atccattttg ctgcctttcg cgcctggctg cagttcgata taaaccccag
    21181 acagcagggt tcccagaccg ctaatccctt ctcgaccaat ctgcggtttc accacccaaa
    21241 agacggtgtc tttatgcagc aatttttcca taccggaatt cagccgcgct ttgatttcaa
    21301 cgtgcgtcaa atcatcagcc agtgtggcgc tttcaaccac gccgacgtca acgctacggc
    21361 ttttaatggt ggttttgcca ccttcaattc cttccgcatt cgcggtgatc agggtcactt
    21421 ccggtccctg atggctgtaa tgataaaaaa gaacccaggc cccaatgagc gccgtgacga
    21481 taggaaatat ccacacggga gaccagttct tcactttctg gattttggct tccccattat
    21541 tagattccat actgtcagga ctcctcatgc tctgattcag gttgacgatc ccacgacaaa
    21601 cgcgggtcaa acgtcatagc agaaaacatt gtcattatga cgactaaagc aaacattaat
    21661 gcacccattg ccggataaat actcattaaa cctcccatac gcaccagcgc cgagagcacc
    21721 gcgataacga aaacgtcaat catcgaccag cggcctacaa actcaacaac ttcataaatc
    21781 aaatgcattc tttcactgtc gcgcttgcca tgccctttgg catcccaaca cagccacgcg
    21841 atggcgatca tctttaacgt tggcaccata atactggcca gaaagatcac cgcagcgacg
    21901 ggataagagc cttcgctcca taacagaatg accccagcga gaatcgtcga cggcatcttc
    21961 gagcctaata aatccgtcac catgatgggc aaaatattag ccggaagata cagcatgatg
    22021 gacgttacaa gcagcgcgag tgtccactgc aggctgtttc tacgccgaac gtaccctttg
    22081 gtactacaac gcgggcacac gggttcatca gcaggaagga ttgccgtaca acaggagcac
    22141 gaacgcagcc cctgacgtat ccccgtgacg cctggtttta gcggctggcg cagttctggc
    22201 atcggggcga tgtcgtccca taaccagcga cgatcaacgc actgaaaagc gcgcagttgc
    22261 aggacacaaa ataaacacca ggggagaaag ctgctgccta ccccaatgct gccgtaagcc
    22321 atcagtttaa cgaaactgac cagcacacca gcgaggaaaa tctccgccat tccccaggtt
    22381 ttgagttgaa aaagcacccg tgccagttgc tcttttaaac ggaccggtaa ttccgcgcga
    22441 ttcaccagta acagaatggt tatcagacaa aacgcgggaa ccagttgcac aaacaacagg
    22501 aaaaaggtgc cgaggctggc gtagtcctca gaaaaaagca cgccgggaat ttccagtaat
    22561 gtaatttcac tggtaactcc tgcaacgttc atattcacaa aaggaaacaa gttggacagc
    22621 aacagcatga acagtgcagc caacgcatag gcggtgggac gctgccgagg ggcatcccac
    22681 gccacggtta acgttgtgcc acaccgggga catgccgctt tctgaccatg ctcaaggcgc
    22741 ggtaacgcca ccagcatgtc acactgcgag cacaggatgt gcttcgcggc atgatgatgt
    22801 tcgcacattg gtactcctta atgatgcgct attttacaga tgactaacaa agtgttggtt
    22861 ttcatgcctg ataaacccag ttcatcaggc aattttaccg ttgtcagctg tctctgttta
    22921 aatcgactat tttgcgatca gccaccattt tttaacgctt caagatactc ccagcgttca
    22981 aaggcttgct ccagctcctg ctctgcagca gccatatcgg cgagcacttt ttgtgtctgc
    23041 tcatgtggct gactgaagaa ggaagcatcc gccacctgcg tctgtagggc ttccagcttc
    23101 gcctccagat cttcgagcaa ttgcggtagc tgctccagtt cgcgctgcaa tttatagctt
    23161 agtttgctac tgctgcgttt tacagtttct gcttttgccg cagcggcttc ttcggttttt
    23221 ttcaccgcag gctgtttgag cgccacatac tgctcttgct gaccacgggc atcatgataa
    23281 ccgccgacat aacgaccaat tttaccgccg ccttcgaaga tccaacattc tgtaacggtg
    23341 ttatcgacaa actgacgatc gtggctaacc agcaataccg tgccctgata gctgtcgatc
    23401 agttcttcca gcagttccag cgtttcgaca tcaagatcgt tggtcggttc gtcaagaatc
    23461 aataagttgc ttggtttgag gaacaaacgc gccagcagca agcggttccg ctcaccgcca
    23521 gaaagcgcac gtaccggcgt catcgcccgt ttcggatgga acagaaagtc ctgcaaatag
    23581 cccaatacgt ggcgtggctt gccgttaacc atcacctctt gcttaccttc ggcaaggtta
    23641 tccatcaccg ttttatcggg atccagttcc gcgcggtgct gatcgaaata agccacttcc
    23701 agtttggtgc caacgtgaat acgcccgctg tccgcttgaa gctgaccgag catcagtttc
    23761 agcagcgtgg ttttgccgca cccattcgga ccaatcaggg caattttgtc gccacgtaga
    23821 acctgggccg aaaaatcttt caccagttgc ttaccgtcaa cctggtagca aacgtcttcc
    23881 atttcgaaaa cgatcttacc ggagcggctg gcctcttcca cctgcatctt tgcggtaccc
    23941 atcacttcgc gacgttcacc acgctcgcga cgcattgctt tcagggcgcg tacgcggcct
    24001 tcattacggg tacggcgtgc tttgatcccc tggcgaatcc acacttcttc ctgcgccagt
    24061 ttgcgatcga actcggcatt ttgtagttct tccacgcgca gggcttcttc tttttcctgc
    24121 aggtactggt cgtaattccc cggataggtc accagcttgc cgcgatcgag atcaacaatg
    24181 cgcgtcgcca tattgcggat aaacgaacgg tcgtgggaga tgaaaataat cgtcccattg
    24241 aaggttttaa ggaacccttc cagccagtcg atggtttcga tatccagatg gttagtcggt
    24301 tcgtccagca gcaatacgcg cggattgctc accagcgcgc gtcctaatgc cgctttacgc
    24361 aaccagccgc cggaaagcga cgacagcgca acattaggat ctaaccccag ttgcgccagc
    24421 acttcgttga tgcggttttc cagctgccac aagttgtggt gatccagctg ttcctgaacc
    24481 ttcgccagtt cgttgagatt tttctcgctc gggtcgttca tcaccaggcg cgaaatatcg
    24541 tgatagcgtt tcagatattc cgcttgttct tcaatgcctt cggcaacgaa atcataaacg
    24601 ctaccctcaa cgttacgcgg cggatcctgt tgcagacgag ctacaatcaa atcttgctcg
    24661 taaataatgc gaccgtcatc cagcccttgt tcacggttga ggattttcat taacgtcgat
    24721 ttgcctgcgc cgttgcggcc taccagacaa acacgttcat tatcttcgat atgcagttct
    24781 gcgttatcga gaagcggcgc gtcgctgaac gacagccagg cgccatgcat actgattaat
    24841 gacattacta ttcctttcag gctgcggtaa tcagccagca gttgtggatc tggcggttac
    24901 gggcgaaatc ctgggagagc gttttttgcg taatttcttg tgctttcagt cccagttttg
    24961 ccaggccgcc gagatccata cggaagccac gtttgttgtt cgagaacatg atcgtcccac
    25021 ctgcacgcag cagacgtttc aaatctttca tcagcgccag atgatcgcgc tgaacatcaa
    25081 acgcatcttc cattcgtttt gagttagaga aggttggcgg atcgataaag atcagatcga
    25141 actgttcatt tgcctcacgc agccacgcca ggcaatcggc ctgaatcagg cgatgcgcac
    25201 gcccggtcag gccattcaga cgcaggttgc gttctgccca ctccagataa gtacgcgaca
    25261 tatccacggt ggtggtgctg cgtgcaccgc ctaatcccgc atgcacggtg gcgctgccgg
    25321 tataagagaa caggttgagg aaatctttgc ctttgctcat ctggccgagc atacgacggg
    25381 cgatacggtg atcgagaaac agaccggtat cgaggtaatc cgtcaggttc accaacaagt
    25441 gagcgttata ttcggtaact tcaagaaact cgcccttctc gcccagtttc tggtactgat
    25501 ttttgccctt ctggcgttca cgggttttca gcaccagttt gtttggcgca atccccagta
    25561 ccgaaatggt tgcagcgata atatcgaaca gacgctgacg cgctttgtga gcatcaatag
    25621 tttttggcgg cgcatactcc tgcaccacca cccagtcggc ataacggtca acggcaacgt
    25681 tatattccgg tagatcggcg tcatacaggc ggtaacattc aattccttcc tggcgagccc
    25741 acttctcgaa ttttttgagg ttcttacgca ggcggttggc gtagtcttcc gctaccatcg
    25801 ccggtttgct gtctggagtg ctttcggcaa cgtggtaatt tttctgtacg cagtccagcg
    25861 ggccgttttt cgccttgtac tgtttgtctg cacgcagctg caagcagctt agcagatccg
    25921 gcgaggcact aaacaaagag agattccagc caccaaactg gtttttcatg atccgaccca
    25981 gcaggctatg cagcgcaatc agcgccggtt cgctgtccag acgttccccg tatggcgggt
    26041 tgctcaacac tgtgccgtac ggccctttcg gcagcggatt ggtcagttgc gcgacatctt
    26101 tcacctcaaa ggtgatcagt tcaccaatcc ccgcaagacg ggcgttagtg cgtgcacgtt
    26161 gaatcacccg tgcgtcgctg tccgaaccgt aaaaatgaga gctatactca gccaggcctt
    26221 tacgggcgcg agtttgcgct tccgctttca cttcctgcca gatagcttca tcatgctgcg
    26281 cccagccgct aaagccccaa cgcccacggt gcaagcctgg tgcgcgatcg gtcgccagca
    26341 tcgccgcttc aatcagcaac gtaccggaac cacacatcgg atcgagcagc ggtgttcctg
    26401 gctgccagcc ggatcgcatc acaatagcgg ctgccagggt ttctttgatc ggcgcaatac
    26461 cagcacgatc gcgatagcca cgcagatgta aaccatcacc actcagatca agagcgatac
    26521 tggcggtttc tttatgcagc cagacgttaa cgcggatatc cggcgcatcg cgatcaacat
    26581 ttggacgcgg cagatttttc cgcgtgaaag catcgacgat cgcgtctttc actttcatcg
    26641 cgccgtactg actgttgcgg atggtgtcat tcaaaccact gaagtggaca gcgaaggtcg
    26701 cgccaggatt aaacatctct gtccagttga tcgcctgaac accgagatag aggtctaaat
    26761 cgctgtaaac cttacactcg cccagcggca acataatacg cgaggccagg cggctccaca
    26821 tcaggctctg gtaaacaagc cgtgtgtcgc ccttgaaatg gaccccaccc tgaaccacct
    26881 ggcattcaac ggcccccagg ttttccagtt cagtttttaa cagctcttcc agcccacggg
    26941 ccgtactggc aaacagagaa ttcatatcgt cacttttact ctaagagcct accgcagtag
    27001 gcgtaattgt tgcagtcagt ctggacatgg acagcgcgga gaaaccggag cgtacacaag
    27061 tacgtgagga tttcgagcac ttccctgggc caaactgaca aataaaatag cctaacggga
    27121 caggcgctaa gaaaattgtt gcgcattata gctaataagc gcgtcatgtc ataaagttga
    27181 gggcttattt tcatttgagg accgcaccgt ggcgacatta acccggcttt ttattcatcc
    27241 tgttaaatcg atgcgcggca ttggtcttac acataccctg gcagatgtga gtggtctggc
    27301 cttcgatcgc atctttatga tcacggaacc tgatggtacg tttattactg ctcgccagtt
    27361 tccgcaaatg gtacggttta ctccttcacc tgtgcatgat ggcttgcatc tcaccgcacc
    27421 agatggcagt agcgcatatg ttcgttttgc tgatttcgcc acacaagacg caccaaccga
    27481 agtttggggc acacatttta ccgcgcgaat tgcgccaggc gcgatcaaca aatggctaag
    27541 tggatttttc tcccgcgaag tgcaattacg ctgggtgggg ccacaaatga cccggcgcgt
    27601 gaaacgccac aacactgtac ctctgtcatt tgctgatggc tatccttacc ttcttgctaa
    27661 cgaagcctcg ttacgtgatc tccaacaacg ttgtccggcc agtgtaaaaa tggagcaatt
    27721 ccgccccaat ctggtggttt ccggcgcgtc agcctgggaa gaagatcgct ggaaagtgat
    27781 tcgcattggt gatgtggtgt ttgatgtggt taaaccttgt agccgctgta ttttcaccac
    27841 cgtcagccca gaaaaagggc aaaaacatcc ggcaggcgaa ccattaaaaa cattgcaatc
    27901 tttccgcact gcccaggata acggcgatgt cgattttggt cagaatttaa ttgcccgtaa
    27961 tagcggcgtg attcgcgttg gcgatgaggt ggaaattctg gcaacggctc cggcaaaaat
    28021 ttacggcgca gctgccgctg atgataccgc caacatcacg caacaaccgg acgcaaatgt
    28081 agatattgac tggcagggac aggcatttcg tggaaataac caacaggtgt tgctggagca
    28141 attagaaaat cagggaattc gtatccctta ttcttgccgc gcgggcattt gtggaagttg
    28201 ccgtgttcag cttttagaag gcgaagtcac gccgctaaaa aaatcagcaa tgggcgatga
    28261 tggcaccatt ctttgctgta gctgtgtacc gaagactgca cttaagttgg cgcgttagac
    28321 tgcctgttcg aggctgaagc tgtcaacatt aacctgcggt ttcagcctgt cgttcatgat
    28381 tttaatggcg tcgcccaact gcatcgcgcg acccgcaata acaacgcagg gttgtgccag
    28441 caggcatagt gcggcatttt cgccagattc aacgaccagc aaattgacct gctcgtgagt
    28501 atcgctcaac caaacgcagg ccgcatcgcc cggcatgggc acccacattt caccatggga
    28561 aacaaaatgc cagcttttcg gcatttgcgg tttgagatag cgaattgcta ccagtgcatt
    28621 cagcaccagt tcggcttttt gttctttaga taaattaaaa tcgcggcact tttcttcaaa
    28681 agagaaatag agcgcggcat cgtcaacgca aaagcctgcg ggagaaaagg catctggagt
    28741 caacatcttg cgcgcaaaac gtgagcgaaa tagcatacca ttggctaaat cgagcatcat
    28801 tcgatcgtgc tcttcatcgt aataccaacg ccagttatcg tctggtttaa ttcgcatctg
    28861 cctctcccct accctataaa accgcagcaa cacaatccat tgtcgcttcg cttaataacc
    28921 tcaacaataa aagtacaaaa tgtctaaata agcaacagcg atgaatataa aacaaccagg
    28981 gctgaaaata aagccctgat tatcttataa agaggaattc gaagaaataa ttagatatgg
    29041 gtaacgattt ctttaatcag cggcggacct ttaaaaataa aaccagaata aatttgcacc
    29101 agtgaggcac ccgcagcaat cttttcacgc gcagcgataa ccgagtcgat gccgccaaca
    29161 ccgatgatcg gtaagcgacc gtttaattcc agtgacaagc ggcgaataat ttcggtgctt
    29221 tttaactgaa gcggacgacc acttaagcca ccggtttgat cgcaattttt cattccctga
    29281 acaagagaac gatcgagtgt ggtattggtt gcaataacgc catcaatatt atggcgaact
    29341 aaactatcgg caacctggat caattcttct tcagaaagat ccggcgcgat cttcactgcg
    29401 atcggcacat atttatggtg catcgcttgc aaatcatttt gcttattttt aatcgcggtt
    29461 aagagatcat ccagcgcttc accatattgc agcgtgcgta atcctggggt attcggcgat
    29521 gaaatattga tggcgatata tcccgcatag gcatagattt tttccataca aatcagatag
    29581 tcatctttgc cctgctccac tggcgtatct ttatttttgc cgatgttaat acccaggacg
    29641 ccgtcataat gggccttttt tacgttctct acgaggttat caacgccaag attattaaag
    29701 cccatacggt tgatcaaacc ttcggcatct accagacgaa agagacgcgg cttgtcatta
    29761 cctggctgtg gacgtggcgt gacggtaccg atctcgatcg atccaaatcc catcgcgcct
    29821 aacgcgtcaa tgcactcccc gtctttatca agaccggctg ccagaccaag cggattttta
    29881 aacgtcaggc ccatgcagtt aacaggtttc gcaggcactt tctgccgcac cagtgcttca
    29941 aacggcgttc ctgtaatacg gcgtaattgc tgaaaagtaa actcatgagc gcgctctgga
    30001 tcgagctgga aaagggcttt acgaacgaag gggtagtaca tgaactctcc tggattcccg
    30061 gtgtgcaaac cgggggcgta ttatgtgcga gttcggacca aaagggaatt gacctgcggc
    30121 aaaaaaaacg caaacgtttt ccgaacctgt ttcagggcag attttgctcc ttctttgcac
    30181 tcaacccgcc gtaatcattt atcgcccgcc aggcgacatg acacggtgcg attgcctgac
    30241 accagcttgt ctgacgctgg cttttaggcg gcacaactcc cgcattactt aacgctttac
    30301 catttacgct caactgaatc aggttaatgt agtaaggcgt tgggttagtg agttgtacac
    30361 cctgcgaatt ccgtgtccag cgtaattgtt gataagcttc cagcgggtcg cccggcaaac
    30421 cttcgggtcg ccagaacagt ttaaataccg agcgcatcgc cacttttacg ctctgatttt
    30481 caactttgcc ggatggcacg ctggcaatgc ttaactcaaa tagcgtttcg cgatccacag
    30541 gcaagatgtc gctttccgtt ctcagcaagc gcaacgtgcc tgtagtaccg ggcttcagga
    30601 gaataagtgg cggagcggcc agtaatggtg ctggcactgt cgacgcttcc cccccggccc
    30661 aacgcgttgg gcggttgatt ttactgttaa taagccagga ttcctgactg gtattagtca
    30721 gtaaaataga tatcgattct ctgtctgccg gaaagataaa tcgcgttccg ccgaccacca
    30781 ctcctgcccg caattcaccc gccaccagca gcgataaaac ggcgaaaatc aagagcgcca
    30841 atctgttcca cgtattagtc ataatgaagt acaaatgtcg cgctggcatt cactttaccc
    30901 gactgcacat cgctattgac aggtcgcaat tgcgcataaa aagttaatgg caccttaccg
    30961 ctatgtaggc tgtagtcttt actcgcctgg ttaagcggaa tcaatatctt cgcatcatcc
    31021 agaatcgcaa ttgccagccc gctggctgca tccataccac tttcaatgga cagcatttcg
    31081 ctctgcactc cgtcgggggt tcctgaaaag gtgacgtcaa ccgtgctgac atctgttccg
    31141 caatcctgca aattaatgat gaaagatttc tccgggccat aattcccgtg agaaaaacgt
    31201 gtcagcgaga catcgcctaa ctgcacattg atttgcttgc tatcctgtgc cagcacgcat
    31261 gtattacgac gaaacgatcc gctaatgttg accgtgccat catgagcaaa taactcacta
    31321 ctccccgtta atcccagtaa gcacagaatc cagattatcc gtttcaacat cactccttca
    31381 ctggtaaacc agatcaaaat agagaaccgc cgtcgcatta ccgcccgttg ctcccgcctt
    31441 tgtggactta taacgaagtt gatatttgag tgtgttatcc ccggctttta agggcgtaag
    31501 aggctggacc tgattgagcg ggatttcttg ctgactttgc gcatcaagaa tttgcacggc
    31561 aatccccgtt gccataccac cgcttccgcc tgtgtcagtt agtttaagca gtgtcggcgc
    31621 aagggtgtca gcctcgccgc taaaaaggac gtccgcgcca gtaataccag cggcacaacc
    31681 ggtgatatcg atattcaaat cagctgcagt gcttacggta ttcgcggcct gaaaatcact
    31741 gatattaaaa tcgccaatat gaatattttg taatgcactt ttggtggcga catcacagcc
    31801 ctgatcgacc acattcccat aaatatgcat actgacttgt tcaggcccag ccatcccgac
    31861 atggatgtta agaaggctaa agaacaaaat aacgtactga tatatcgttt ttttcttcat
    31921 acgttcacgc gttaatcaaa atttattctc accgttgccg tggcttcaaa tcgcccggtt
    31981 tctggtggta cgcctgtcgt gctgactgga tagacattca ttcgcgcgat actgtctgca
    32041 tcatcaggaa tagagagcgg tagtgtccca ccagcagggg aaataatgtt gttctggcta
    32101 tcggtcacca ccacgcccac gccaggacgt gacgtcttaa tcgcctgggg atagctgggg
    32161 tctgaggttg ccgtcagact caggttgacg ttaactaacc cttcgtttcc cgtgttacac
    32221 ctgactggaa ttgcgagttc tgccgttctg gccccaaggg gtttatgccc tacaacacga
    32281 aagttagccg caaatacgtc gcccagatcg acctcgatag tctctccggc attgacggaa
    32341 catgagccaa gtgaattgat cgtaccagac aggtaataca ctaaaaccgg tgtacctgta
    32401 gtcgtgcagg aatcacctgc gggtatgttg tagcattcaa ataatcgcgc cagcgccata
    32461 ggaggaataa cgaccgaacc aacgaacggc tgcaatattt tcagggaaaa gctaccgcta
    32521 ttaccgctag ccccctggcc acctaaacgt tgaccgccgg gatcgcaaat atgccatcgt
    32581 gtatctgccc ctgtgcccac gttttcgaaa ggaaccgcag taggagaact gcctttaacc
    32641 tgcaataaaa ctttggcggc aagatagtca ttaattttgt accagttgtc gcctaaatag
    32701 actaacggtg aatccgcggc gaatgcccat cggccacttc tgacattgtc tttatcgcaa
    32761 tcacaggaaa cataataatc attcgattcg ttccactgat aaaatttgtc tttaactact
    32821 ccagccgtat tttcttcttc ctccagggtt ttaacgccaa aattgacgcc aatatacgga
    32881 ttcccctgag tggtatgaca ctgcccgcta taagcagcag tttgagcgca ccatagcatt
    32941 aagacggcgg caaaaaatag tcgtagtaat aacacgcatt tttctccaaa gattatctgc
    33001 atatggcatc cattcgcgta atcgcagagt taatttgttg ttcggtaagc tggtaactga
    33061 tatgacaaat ttgatcggga gcttcgcccc agcgtacagt tatcgctccc tgagcgggca
    33121 aaccggacat atagagcgag ccatcatcat caataatgcc gctattagtc tgtatgccgt
    33181 ccagcgtcgc tatcgcgcca aaacgcagcg gtataccctg ctttgatgtt ttcattaata
    33241 cccttgctcc cgcatgggca ttgaactccg ccagaaccaa cgcccctttg gtgggaacca
    33301 cgttgactac tgcattttca agatccacgt tacgttttaa cgatgccgca tcaagtgcga
    33361 ttcgattttc ccgataagaa gtggcatagg ggacaacggt atatccgcgc cagtcagttt
    33421 tcagcccggt gctgttcgcc acacgagtat tctctgcgcc tggtgctgca atcagcacgt
    33481 tagtttcccc cagcgattgc cccagggtaa tgccctgtga atgggcaact aaactgcctg
    33541 aaagagcgta gttcagttgt tgttgactgc cgttatcgct gtagttgtag cccactcggg
    33601 catctgcaaa cgcccctttg tagtccatgc tggcgctacc attagccgtt ttcccctcgc
    33661 tgttatatcc ctgttgcacg ctgtagctca ggttaccgtc atcgagtagt gtctccgtta
    33721 aacccaggtt ttgtgtaatg tgcccttcgt tatcgatact ggtgttagag gtcatccagg
    33781 cgtaattttt tttcctggta tagctatcat tacttctggg gagcaacaga ttgagcggca
    33841 gggaaaaatt tagtgcaaac acctgatcag catcaggttg accacgggac ttactgtagt
    33901 tccaggaaac ccccagcgag agatctttaa tttgcgtgtt gtagccaaac tgtaatagcc
    33961 gatcctgttg atcggtatgc cagtaagttt gctggctacc acttaaataa aacgaaccgt
    34021 actcgcctaa ttgctgggag atattgacct gcagtttgcc acgtttggtg taaaacaaat
    34081 tgtaataacg cgaccacatc ggcgtatctt catcatcacc gtcattaaat tcgtagccat
    34141 ccatatgttt atacatggtg tcggaaaggg tgtagaaacc cgacgtcgaa tagcgatagc
    34201 ccatcaattg aaaattagtg cccaattggt tgagcgtttt ggcatacaga aaacgatatg
    34261 actgaccttt atgttcgctt tggtctgcca gcgtactctt cgcctgggtc gcatcgaacg
    34321 aaattgctcc gaaatcgcca aggttaaatc ccagaccaaa catggcggca cggtaatatt
    34381 cagcatattg tccaccacca taccatgtcg ttccccacgg tccgccccac tgcaacgtgg
    34441 cctgggcaaa tttgctctcc tgctgttcat tactattggt tctgtatttc gccagtgtca
    34501 ccgcgtattt gattcgcccc tgacgctgga gtaatggcac gctggaaaac ggtacgctgt
    34561 agctattgac gctgccgtcc gcttctttga tttcaactaa caaatcaccg ctcgacgacg
    34621 tggaatagag atcactaatt tcaaaagcac caggcgatac ataagtttgg taaatggtgt
    34681 aaccattttg cttaatcgtt atctgggcat tgcttttggc gataccacgc accacaggcg
    34741 caaacccttt caggctgtct ggcagcatgt tgtcatctga cgccagctgc acaccacgaa
    34801 agctgacact atcgaaaaaa tcgcctgcag tataatcatc acctacggtc agttcgcctt
    34861 ttagtggaat aaccgcccgc tgcaaatagc tgctgagatt cttccattct gcggtttggc
    34921 cactactgcg actccatgtt gaattgttgc gcaatctcca tgggcctaaa ttaacgccac
    34981 tgtttaaatt cagaaaatag ctgtcgccag aatcactgtc tgcgctgcta tgaatactgt
    35041 tagccccgct aaacgaatat cccagtaata gcgcgttgat cccttcatcc caacgttcag
    35101 gggggacata accacgggca ttacttttga tggcgatttg cggtactgaa atttccagac
    35161 ggagtttatt cacattaaac gttacgctgg catccggaat aatctccgcc aatggcacac
    35221 acgcttgttt gtccagttgt ttaaaagccg gaaacgcatc agtattaaca cccattgcat
    35281 ccaggctttc agtcgtcaaa caggcggcaa tccccttctc tggcgttatc tcaacaaaat
    35341 tgacgttgcg cgtatctaca attgtctgat tcagaacaat atcgacacga taaacccctg
    35401 gcgttatttt ttgtcctttt tcgaaacgag acaaatcggc caccattgag ggatcatctt
    35461 tgaaaaaagc aggatcgaac caggtttcag cttgtgcatt gaacgctgcc gacacaaaca
    35521 ccaccagccc accaataaac gatggcactc caccagagct taacaggctg tgttgtcgat
    35581 gagttctata catctcgctc tcttaactga agacggctta cgatttacag ttgagttgcg
    35641 gcgtttccgc cccgtagtca ttgatggttc gatacgttac gctgctattt ttggccccct
    35701 ttggcagaga aaattggtat ttatcaaagg gggaaatcat ttgattttta actacttttc
    35761 cacctatttt caggtctgtc agggtaatga aataaggtgt tgggttactg accgtaacca
    35821 tgtcagctga cgaagtgcaa cgcagtttcg ccggagcctc gcctaaggga tacgctaaac
    35881 cttttggtcg ccagaaaagt ttgatgacgc tttgtgtggc aatcttcagg gtatttccct
    35941 cttcccgctt cgttgttgaa gggactgccc gtacgctagt gaagaaaagg gtttctctgt
    36001 ctttcgccaa cggcgcacca atgtacataa tgcgtaacag attctcatta gccggattca
    36061 gcacaaatag cggtggtgta ataataaaat ctgctgattt tttattatct ggggtcatca
    36121 cccatgactg aataagataa ggaacatccg gactggtgtt acgtacaccc agtgaaattt
    36181 gctcttcacc ttctgaataa acaagacgtg ttgctgcaag tccaatcccg cctttacctg
    36241 ccgcccatgc ggatgagcag ctgagtaata ttgccgttaa acttacggtc acaattccct
    36301 ttgttatgca agttttcata aatagtcact cttaaaaaa
//
LOCUS       DAZFJF010000035        36249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFJF010000035 DAZFJF010000000
VERSION     DAZFJF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.035"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            105..719
                     /gene="evgA"
                     /locus_tag="ACHKAU_001950"
     CDS             105..719
                     /gene="evgA"
                     /locus_tag="ACHKAU_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO5531021.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            724..4317
                     /gene="evgS"
                     /locus_tag="ACHKAU_001951"
     CDS             724..4317
                     /gene="evgS"
                     /locus_tag="ACHKAU_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO5531022.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4373..5518)
                     /gene="yfdE"
                     /locus_tag="ACHKAU_001952"
     CDS             complement(4373..5518)
                     /gene="yfdE"
                     /locus_tag="ACHKAU_001952"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO5531023.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5592..6536)
                     /gene="yfdV"
                     /locus_tag="ACHKAU_001953"
     CDS             complement(5592..6536)
                     /gene="yfdV"
                     /locus_tag="ACHKAU_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO5531024.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6606..8300)
                     /gene="oxc"
                     /locus_tag="ACHKAU_001954"
     CDS             complement(6606..8300)
                     /gene="oxc"
                     /locus_tag="ACHKAU_001954"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO5531025.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8354..9604)
                     /gene="frc"
                     /locus_tag="ACHKAU_001955"
     CDS             complement(8354..9604)
                     /gene="frc"
                     /locus_tag="ACHKAU_001955"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO5531026.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10117..10749)
                     /gene="yfdX"
                     /locus_tag="ACHKAU_001956"
     CDS             complement(10117..10749)
                     /gene="yfdX"
                     /locus_tag="ACHKAU_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO5531027.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            11045..11320
                     /gene="ypdI"
                     /locus_tag="ACHKAU_001957"
     CDS             11045..11320
                     /gene="ypdI"
                     /locus_tag="ACHKAU_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO5531028.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(11397..11639)
                     /gene="yfdY"
                     /locus_tag="ACHKAU_001958"
     CDS             complement(11397..11639)
                     /gene="yfdY"
                     /locus_tag="ACHKAU_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO5531029.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            11992..12912
                     /gene="lpxP"
                     /locus_tag="ACHKAU_001959"
     CDS             11992..12912
                     /gene="lpxP"
                     /locus_tag="ACHKAU_001959"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO5531030.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            13268..13339
                     /gene="ypdK"
                     /locus_tag="ACHKAU_001960"
     CDS             13268..13339
                     /gene="ypdK"
                     /locus_tag="ACHKAU_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO5531031.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(13404..14642)
                     /gene="alaC"
                     /locus_tag="ACHKAU_001961"
     CDS             complement(13404..14642)
                     /gene="alaC"
                     /locus_tag="ACHKAU_001961"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO5531032.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            15019..16716
                     /gene="ypdA"
                     /locus_tag="ACHKAU_001962"
     CDS             15019..16716
                     /gene="ypdA"
                     /locus_tag="ACHKAU_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO5531033.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            16731..17465
                     /gene="ypdB"
                     /locus_tag="ACHKAU_001963"
     CDS             16731..17465
                     /gene="ypdB"
                     /locus_tag="ACHKAU_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO5531034.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            17478..18335
                     /gene="ypdC"
                     /locus_tag="ACHKAU_001964"
     CDS             17478..18335
                     /gene="ypdC"
                     /locus_tag="ACHKAU_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5531035.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18338..20833)
                     /gene="ptsP"
                     /locus_tag="ACHKAU_001965"
     CDS             complement(18338..20833)
                     /gene="ptsP"
                     /locus_tag="ACHKAU_001965"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5531036.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(20858..21895)
                     /gene="ypdE"
                     /locus_tag="ACHKAU_001966"
     CDS             complement(20858..21895)
                     /gene="ypdE"
                     /locus_tag="ACHKAU_001966"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5531037.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(21895..22980)
                     /gene="ypdF"
                     /locus_tag="ACHKAU_001967"
     CDS             complement(21895..22980)
                     /gene="ypdF"
                     /locus_tag="ACHKAU_001967"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5531038.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(22995..24242)
                     /gene="fryC"
                     /locus_tag="ACHKAU_001968"
     CDS             complement(22995..24242)
                     /gene="fryC"
                     /locus_tag="ACHKAU_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5531039.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(24264..24590)
                     /gene="fryB"
                     /locus_tag="ACHKAU_001969"
     CDS             complement(24264..24590)
                     /gene="fryB"
                     /locus_tag="ACHKAU_001969"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5531040.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(24809..25774)
                     /gene="glk"
                     /locus_tag="ACHKAU_001970"
     CDS             complement(24809..25774)
                     /gene="glk"
                     /locus_tag="ACHKAU_001970"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO5531041.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            25978..27234
                     /gene="yfeO"
                     /locus_tag="ACHKAU_001971"
     CDS             25978..27234
                     /gene="yfeO"
                     /locus_tag="ACHKAU_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO5531042.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            27349..27675
                     /gene="ypeC"
                     /locus_tag="ACHKAU_001972"
     CDS             27349..27675
                     /gene="ypeC"
                     /locus_tag="ACHKAU_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO5531043.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(27816..29054)
                     /gene="mntH"
                     /locus_tag="ACHKAU_001973"
     CDS             complement(27816..29054)
                     /gene="mntH"
                     /locus_tag="ACHKAU_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO5531044.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            29390..30592
                     /gene="nupC"
                     /locus_tag="ACHKAU_001974"
     CDS             29390..30592
                     /gene="nupC"
                     /locus_tag="ACHKAU_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO5531045.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(30642..32831)
                     /gene="pdeA"
                     /locus_tag="ACHKAU_001975"
     CDS             complement(30642..32831)
                     /gene="pdeA"
                     /locus_tag="ACHKAU_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5531046.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(33039..33114)
                     /locus_tag="ACHKAU_001976"
     tRNA            complement(33039..33114)
                     /locus_tag="ACHKAU_001976"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33079..33081),aa:Ala,seq:ggc)
     gene            complement(33154..33229)
                     /locus_tag="ACHKAU_001977"
     tRNA            complement(33154..33229)
                     /locus_tag="ACHKAU_001977"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33194..33196),aa:Ala,seq:ggc)
     gene            33450..33809
                     /gene="yfeC"
                     /locus_tag="ACHKAU_001978"
     CDS             33450..33809
                     /gene="yfeC"
                     /locus_tag="ACHKAU_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5531047.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            33811..34203
                     /gene="yfeD"
                     /locus_tag="ACHKAU_001979"
     CDS             33811..34203
                     /gene="yfeD"
                     /locus_tag="ACHKAU_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5531048.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(34255..35670)
                     /gene="gltX"
                     /locus_tag="ACHKAU_001980"
     CDS             complement(34255..35670)
                     /gene="gltX"
                     /locus_tag="ACHKAU_001980"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO5531049.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            35929..36004
                     /locus_tag="ACHKAU_001981"
     tRNA            35929..36004
                     /locus_tag="ACHKAU_001981"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35962..35964,aa:Val,seq:tac)
     gene            36049..36124
                     /locus_tag="ACHKAU_001982"
     tRNA            36049..36124
                     /locus_tag="ACHKAU_001982"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36082..36084,aa:Val,seq:tac)
     gene            36129..36204
                     /locus_tag="ACHKAU_001983"
     tRNA            36129..36204
                     /locus_tag="ACHKAU_001983"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36162..36164,aa:Lys,seq:ttt)
BASE COUNT         9516 a         9141 c         8484 g         9108 t
ORIGIN      
        1 aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta tgtctgtatt
       61 actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac gcaataatta
      121 ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa aacgatattg
      181 aaatcttagc ggagttgact gaagggggaa gcgccgttca gcgggtggaa acacttaagc
      241 ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag gtgttagaaa
      301 cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa aatgaccatt
      361 tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa aaagaaggca
      421 tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat ttccccttct
      481 ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac tccttatcga
      541 aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat gacattgctg
      601 aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg atggaaaaat
      661 tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa atcggctaat
      721 cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc gaccataagt
      781 ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg tgtcacactt
      841 gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa aaatcttgtg
      901 attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca gcaacaggtt
      961 cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat caaattaaca
     1021 ctccgggaat acgccgatca tcaaaaagca atggacgcgc ttgcagaagg tgaagtcgat
     1081 atagtgttat cacatttagt tacttcgccg cctcttaata gtgacattgc tgcaaccaaa
     1141 ccattgataa ttacctttcc ggcgctggta accacccttc acgactcaat gcgaccgctt
     1201 acctcaccaa aaccagtaaa tattgctcgg gtagcaaatt accccccaga cgaggtaatt
     1261 catcaatcgt ttccaaaagc aacaattatc tcttttacaa atttatatca ggcattagca
     1321 tccgtatcag ccggacagaa tgattacttt attggtagta acatcattac cagcagtatg
     1381 atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc gccgcgtcaa
     1441 tataattttt tcttgaccag aaaagaatcc gtcattctta atgaagtact caatagattt
     1501 gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct tgatacagga
     1561 aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca gtggattaag
     1621 caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc tatgacggat
     1681 gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac cttgcaaaca
     1741 ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac acagcttaac
     1801 cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga aaataatgtt
     1861 tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa agcacctgac
     1921 agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta tgagctgcat
     1981 tcgcaattaa aagagatgta tccggaggtt gaatggatac aggtcgataa tgccagcgct
     2041 gcatttcaca aggttaagga aggtgaactt gatgctctgg tcgcgacaca gttaaattcg
     2101 cgttacatga tcgatcatta ctatcctaat gaactttatc attttcttat tcctggcgtt
     2161 ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa ggatattatt
     2221 aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga aaaatggatt
     2281 aaaatgccca atgtgaccat tgacacatgg gacctatata gcgagcaatt ttatattgtt
     2341 acgacattat ccgttttatt agttggcagt agccttttat ggggattcta cctgttacgc
     2401 tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc attccgaaaa
     2461 gcactctcgg attccttacc gaatccaact tatgttgtaa actggcaagg taatgtcatt
     2521 agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa tgcaatgtta
     2581 ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca tgaagtcaca
     2641 gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga taatggcatc
     2701 gagaaaagat gcattaatca ctggcataca ttatgcaatc ttcctgcaag tgacaatgca
     2761 gtatatattt gtggttggca agatattact gaaacgcgtg atctaattaa tgcactcgag
     2821 gtagaaaaaa ataaagcgat aaaggctacc gtagcaaaaa gtcagtttct ggcaacgatg
     2881 agtcacgaaa taagaacacc aataagctct attatgggct tcctggaact tctgtcgggt
     2941 tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc caccggacaa
     3001 tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc gggtaactat
     3061 caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg tcactctttc
     3121 ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt tcctgatcat
     3181 tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt actgagcaat
     3241 gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt tcacattgat
     3301 gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt atcgcaggaa
     3361 gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca gcaaacagga
     3421 tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg cgatttgtca
     3481 ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt agaaattagc
     3541 cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc tgaaaagttg
     3601 agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg ccagctaaat
     3661 ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca caaagtcagt
     3721 atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga tggttttgag
     3781 ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct tacagccaac
     3841 gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg tttgttcaaa
     3901 ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt tgcgcatatt
     3961 gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc gaacgatcta
     4021 caactgatgc aggagattct catgactttc cagcatgaaa cgcataaaga tctacccgct
     4081 gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat tcatcgcatc
     4141 cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca tcagttagaa
     4201 ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa ctctgtaaaa
     4261 gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa tgactaaata
     4321 gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac atttatgatg
     4381 agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc ggcataacat
     4441 gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt cccccggctt
     4501 caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc acacttaata
     4561 acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca gctgcctgcg
     4621 tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg cgtaaaatat
     4681 tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg cataacgcag
     4741 aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta ttgaaaacat
     4801 caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc cctgtcgcga
     4861 tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc gctatatcga
     4921 catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt ccgctgaata
     4981 aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc ggagcatcag
     5041 gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg taggcaggag
     5101 catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg aggcgcgggt
     5161 tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct gggcgaaaat
     5221 tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta tcgtgatcat
     5281 tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa tagagtgact
     5341 gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc ggctcaactt
     5401 taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca ttaaggacat
     5461 gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta tttgtcattt
     5521 gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg taaaatcaat
     5581 aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc cgtgacgaca
     5641 aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac attaaaccgg
     5701 ctggcaataa tgattccgga gaatgccggc ggtaatgcgc ctgccagtac catcatctgc
     5761 agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag tggcatcaga
     5821 atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt atgtgcagcc
     5881 agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat cagattaaag
     5941 gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt tgccagaaca
     6001 ggtgcccata ctactggctc ctttgccgca gaaattaatg cgctcagatt actattcttc
     6061 ttaccatccg ctcctgagga aggattcagc aaatacagac caataggaat agtaattgcg
     6121 ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc accataaata
     6181 ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacccgcaat taatgcacat
     6241 acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccaaacca ggagaaaaag
     6301 aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc aaaaatcatt
     6361 tccctgtttg cccgggtaat agaaacaaat agagccgcag gaagagcgta gtttaatacc
     6421 agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc gctaaaataa
     6481 cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt tagcataata
     6541 gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg agacgatgtc
     6601 agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc actttcagtt
     6661 cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg aataccggtg
     6721 gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc acgaaacgca
     6781 tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggtgatgg tgcaccagcg
     6841 ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat aacgatcgtc
     6901 accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc aaaagcacta
     6961 tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat ggcatagccc
     7021 ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg tttatacata
     7081 tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa ataaatatcc
     7141 tggttctcgc gcaatacgtc gcgcacagca cttaatgcat taaagtaatt taatggttga
     7201 gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg gatatttaaa
     7261 atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc cagcatacct
     7321 tgcatactgg atgcaatatc accaacgact ggcacagcaa tggggcggtt gctgtcaatt
     7381 tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc ttttttaccg
     7441 tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc atttgccagg
     7501 gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat ccctttcgcc
     7561 atagacattg gcaggaatgg aatctgagta ctttcaataa actcacgaag ctgttcatca
     7621 gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc ttttgctaaa
     7681 agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga cggattttca
     7741 actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag gacatttgct
     7801 ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat agcgcgtgcc
     7861 aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc atacggtttt
     7921 gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac gatcgcgcgg
     7981 tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt ggccaatgcg
     8041 gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg tttttgggta
     8101 agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa accaatataa
     8161 cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc tacaacacca
     8221 taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat accatctgtc
     8281 atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg ggagccagaa
     8341 gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca tcgtcgctat
     8401 aacccagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca gctttaatat
     8461 ccggcgtaaa ggcagagaat ttcattggac aaccaacggt cagatatttt ccacgcaacg
     8521 gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt gaaatttctt
     8581 tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc accgcttcat
     8641 gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa atatgtggct
     8701 gtcgtgcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg ccgatggctt
     8761 tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg ttaggatcgg
     8821 tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct gcattaccac
     8881 cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct tccagataac
     8941 ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc aatacggcat
     9001 cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc agcaaagcag
     9061 caagtaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct gcagcgctta
     9121 ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct gcctgagcaa
     9181 cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct ttgatcgaac
     9241 caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc atgtgatcaa
     9301 tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc ttttccatta
     9361 cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta ctgttaagca
     9421 tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc gttacgtcac
     9481 caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc atttgagtac
     9541 aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga agtggagttg
     9601 acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat ttaaggcgta
     9661 atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat ttttaaaatc
     9721 ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg catggtaagt
     9781 agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga gagagtcgcg
     9841 acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa gtaagggtat
     9901 gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg tgatgacacg
     9961 ggattattgg gatttacttt gaggaagata aaatgcgtgt ttatagaacc gcgagcgcca
    10021 aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg gctggaggga
    10081 gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca gtgcttcgct
    10141 gtcgacgatg attccatctt ccgcaccttt aagcgccagg tttgcctcgt agtattgctt
    10201 tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa gcggcatcag
    10261 atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg cgcccttttt
    10321 atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt cgggagttgc
    10381 tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt catcattgag
    10441 attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat ctgacagcaa
    10501 agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa acagagccag
    10561 gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac gctctgctgc
    10621 tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta ccggtgcatt
    10681 atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag ccataattaa
    10741 acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc atattgggtg
    10801 agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct tacagttgta
    10861 gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc ctgtcgcaga
    10921 gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa atattgatta
    10981 attatatgta aatacagcca taacatcgta ggttctcagt gaaccatttt caggataact
    11041 ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggtctcta ttatggcatg
    11101 caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata aagagactga
    11161 aaaaacatca gcgatgacaa ccacacactc tacaaaactt cagccatcac aggcgatttt
    11221 gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca cgcctactta
    11281 tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac atggcaggtg
    11341 aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct gacccactat
    11401 cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc aagaaacata
    11461 aatatccccg tcactagttc attaccgcca gttaaccaca tggtgaagta ataaatgagt
    11521 gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt cagtacctga
    11581 ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa ataaatcata
    11641 gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata gtaagacaat
    11701 ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa atgcaagact
    11761 gttgtgtacg gaaaaatatt tactttgcac gattaataat cagtagctga aagcagtcag
    11821 cgagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct gactagtctt
    11881 tacactcttt acaggaacca ttgtcgtaca tgatggccca accaattgag tgtaccgctg
    11941 tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac tatgtttcca
    12001 caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg gtttgggctt
    12061 ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct cggcacgcgt
    12121 attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg taaaaacctg
    12181 gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc cgaaaacttt
    12241 cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc cgacagtcgc
    12301 gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca aatgcaaaat
    12361 cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg ccgcgtgatg
    12421 ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct gatggaatgg
    12481 gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa taatctgcgc
    12541 ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga tcaggattat
    12601 ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc cacaaccaat
    12661 ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac gatggtaaga
    12721 aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg ctacccgaca
    12781 gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat catgcgcgca
    12841 ccggagcagt acctctggat ccaccgtcgc tttaaaacgc gcccggtggg agaatcgtcg
    12901 ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat aataacctca
    12961 tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa ttattaccgt
    13021 cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta aataagaaaa
    13081 tgtctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc agtttagctc
    13141 agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct ttgcccggtg
    13201 tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagtaatt acgcggagag
    13261 atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt gggccggtac
    13321 ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca tcaggctcct
    13381 gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt gctgctggcg
    13441 ggtagtaaac cgtcggcacg gaacatcgct ttaatcccac gaatcgcctg acgaatacgg
    13501 tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc aaagccaatc
    13561 cccggcgaga cacaaacctt cgcttcgtta aggagcttct tggcaaattc cagcgatccc
    13621 atggccgcat atggttccgg gattttcgcc cagacataca tcgaagcctt cggcatttca
    13681 accatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg tttgtactgt
    13741 tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc cgccacctgc
    13801 aacggcgtaa aggtgccgta atcgtgatag cttttaatac gagccagggc gctgaccagc
    13861 gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct tttcgacagc
    13921 gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga cggcgctttc
    13981 cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac atcgtagcgt
    14041 ttcgccagcg ccacaacctt ttcgaagaac tccagctcca cgcattgcgc ggttggattc
    14101 gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat ggcgcgctcc
    14161 agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg cgccccggca
    14221 atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac cgtgtcacca
    14281 tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat agtgacgatg
    14341 gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg ggaaatggcg
    14401 cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtccgg gcgctgggcc
    14461 acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt acccatgctg
    14521 aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc ggtgatgtta
    14581 aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg agtgtcagcc
    14641 ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc cacaatggtg
    14701 gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa catttttatc
    14761 aatggcttta aatgaggaac tccacttcaa aaacaggaaa tttttctacg ctaacaagaa
    14821 aattaccacc aattttatta acattataat ttcaataaat ttaccaacca gaagtgcatt
    14881 atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa atttatggtt
    14941 tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata gaggtttaat
    15001 ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt cttcgatcgg
    15061 gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt tcgcgaactg
    15121 ttgcacaagt cggcgcactc cccaaaagaa ttgctcgccg ttaccgccat tttctcgctg
    15181 ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt gaacgtacgt
    15241 attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtaggcat cattaccggc
    15301 gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc gatcccctgc
    15361 tttatcacca gcattctggc gggttgtata tcgggctgga tcaacctgaa aatccccaaa
    15421 gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgagacgtt gaccatgatt
    15481 ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc taaaatcggc
    15541 attccaatga tcctcggtag cgtctgtatc ggctttattg tgcttctggt gcaaagcgtt
    15601 gagggcgaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga tatcgccaac
    15661 aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt ctgcgaaatt
    15721 atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca tgtgctggcc
    15781 tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag cccgactacc
    15841 cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc ccaccgcaca
    15901 ccagagattc attccatgct ggtgatccca ttgtgggaga aaggggtcgt gaccggaacg
    15961 ctgaaaattt actactgcca cgcgcatcag atcacctcgt cattacagga aatggcggtc
    16021 ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca gctacgtgaa
    16081 atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca ttttctgttt
    16141 aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc tcgccagttg
    16201 atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga tgagcaaatc
    16261 gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca ggcccgcttt
    16321 ggtgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg cattcccagc
    16381 ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc ttgcaaaggt
    16441 aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat tgcggtgcga
    16501 gataccgggc acggcatcga tccaaaggtg attgagcggg tcgaagcgaa tgaaatgccg
    16561 ggcaataaaa ttggcctgct gaatgtccat catcgcgtga agttattgta tggcgagggg
    16621 ctgcatatcc gccgcctgga gccggggacg gaaattgcgt tttacattcc taaccaacgc
    16681 accccagtcg cctcacaggc tacgttattg ctttgagccg gagtgatatt gtgaaagtca
    16741 tcattgttga agacgaattc ctggcacaac aggaactgag ctggctaatt aaagagcaca
    16801 gccagatgga gattgtcggc acctttgacg acggtctgga cgtgttgaag tttttgcagc
    16861 ataaccgcgt cgacgccatt tttctggata tcaatattcc gtcgctggat ggcgtgttgc
    16921 tggcgcaaaa catcagccag ttcgccaata aaccgtttat tgtgttcatc accgcgtgga
    16981 aagaacatgc ggtagaagcg tttgaactgg aggcgtttga ctacattctc aaaccgtatc
    17041 aggagtcacg tattaccggg atgctgcaaa aactggaagc ggcctggcaa caacagcaga
    17101 ccagcagtac gcctgccgcg acggtaacgc gtgagaatga caccattaat ctggtgaaag
    17161 atgagcgaat aatcgtcacg ccaattaacg atatctatta cgccgaagcg cacgagaaaa
    17221 tgacctttgt ctatacgcgg cgtgaatcct acgtaatgcc gatgaacatt accgaatttt
    17281 gcagcaaact gccgccgtcg cattttttcc gctgccatcg ctcattttgt gtcaatctga
    17341 acaaaatacg cgaaatcgaa ccgtggttta ataacaccta cattctgcga ctgaaagatc
    17401 tggattttga agtgccggtc agtcgcagca aagtgaaaga atttcgccag ttaatgcatc
    17461 tttaatcggg aactttcatg aaagcaccag gcttgcccgc cgatcaacaa tttttcgccg
    17521 atctgttcag cggcctggtg cttaacccgc aactactggg gcgcgtctgg tttgccagcc
    17581 agcctgcctc gttaccggtg ggcagtttat gtattgattt tccccgtctg gatatcgtgc
    17641 tgcgcggcga atacggcaac ctgctggaag caaagcagca gcgaatggtg gaaggagaaa
    17701 tgctgtttat tccggcacgc gcggctaatt taccgatcaa caacaaaccg gtgatgctgt
    17761 taagcctggt gttcgcaccg acctggcttg ggttatcgtt ttacgatagc cgcaccacgt
    17821 cgttgttgca tcctgctcgc cagatccagc ttcccagcct gcaacgcggt gaaggtgaag
    17881 cgatgcttac cgccctcacc catcttagcc gttcgccgct ggagcagaat atcattcagc
    17941 cactggtgtt aagtttgctg catctttgcc gcaacgtggt gaatatgccg ccgggtaatt
    18001 cgcaaccgcg cggcgatttt ctctatcaca gcatttgtaa ctgggttcag gataattatg
    18061 cccagccgct cacccgcgag agcgtggcgc agttttttaa tatcacgccc aatcatctgt
    18121 caaaactgtt tgctcagcat ggaacgatgc gttttatcga gtatgtgcgt tgggtgcgaa
    18181 tggcgaaggc gaggatgatt ttgcagaaat atcatctgtc aattcatgaa gtggcacagc
    18241 gttgcggttt tccggatagc gactattttt gtcgcgtttt ccggcgtcag tttggtctga
    18301 cgccgggaga gtacagcgcc cgttttcagg gctaacgtca gaaggttaat tctgtttcca
    18361 gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa cgactggcgg aagtttttat
    18421 tcaccagttt acgcgccagc tgcgagaaga ctttcacatg attcatccct tcgttagcac
    18481 ccagcgtcag catgatcacc agttcgactt cgcccatttc tgactgccag tcaaccggtt
    18541 tcgccagccg ggcaatgctg atactggaat gacggatcca ctgagatttg gtgtgcggga
    18601 tcgctacgcc aaaaccaacg ccggtggtga caatctcttc ccgctgccag acatcttctt
    18661 ccagctcaaa cggatgttca gtgcgcccgt taacgccgag gttgccgcac aggaactgga
    18721 tcgcctgctc tttattgcta aaatcctgat caacaaagat attctccagc gccagcagtg
    18781 ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc ttcaatttcc tgcgcactgc
    18841 ggcattcaca tgcctgacgc gccagttccc gacacgcctc gctatccagt tgacgaagct
    18901 ggcttttcac cgccggaata cgcgggctac tcatactcag ctcatccagg cccagcccaa
    18961 gcagtagcgg cagataacgg ctttcaccgc ccagttcacc gcaaatgcct acccatttgc
    19021 cccgctgatg cgcagtggta acaatctgct gcaacatgcg caggaacgat ggcgtaatcg
    19081 ggttatacag cggcgatacg cgcgggttat tacgatcgac cgcatacaga tactgggtca
    19141 tatcgttgga gccgatactg aagaaatcga cctcatcgca gaagtgatcg atgatgtagc
    19201 acaccgacgg cacttccacc atgatcccca gcgtaatcgt ctctgcatga cgcaggccat
    19261 cgcgcttaag ctcaacgatc gctttttgga tctcgccctt cacccataag atctgatcga
    19321 gactgtggac catcgggatc atcaactggg cgttgccaaa actggcggcg cgcaaaatcg
    19381 cccgtagttg agtgcggaac aggccagcaa attccgggta aatacgtacc gcgcgatagc
    19441 cgaggaacgg gttctcttcc tggggaatat tcagataagg aatgctttta tcgccaccga
    19501 tgtccattgt gcggaagata atcggcttgt cgcccgctgc cagtagcacc tgctggtagg
    19561 cttcaaattg ctcctgctcg tccggcgcgc tgtcgcgatc catatagagc atttcagtac
    19621 ggaacagtcc aacgccttcc gcgccgttgg caaacgcgcc tggcgcttcc agagcggtgc
    19681 cgatattcgc cgcaatatca atacgcttgt tatcacggga ataggccagc tgcgcggcgg
    19741 cctgcgcctg ttgtttttgg cgtttatccg ccagcgtctg tgcgacctga taataaccgc
    19801 tcaccgcgtc attcgggtta atcgccagca cgccgcactg ggcgtcaagc accgccggtt
    19861 gcccggcata acgggcaatc gcatccagcg gcaggccgct cagtaccggg atcgccgagg
    19921 cgcgggtcag aatcagggta tgcgaggtgc gcccagtttt ctccagaatc atgcctgcaa
    19981 gattttttaa atcgaggctc aaaaactggc ttggggttaa atcctcggct accagaatag
    20041 tcggtttttc cagcaccaga ttgttgcgcg gcttcagttc cggccaggtg atatgcagca
    20101 actgttcgct aatatcgcga atgtcgctaa cacgttcgcg cagataatca ctggcagaag
    20161 cagaaagttt ggcgcaaacc tgctccatat tgctgatgat cgccgccccc agcccttgat
    20221 gctgttctgt catcagacga cggatattgc ccgcgaattc atcatcctga atcagcgaca
    20281 aatgggcgct gaggatagtt ttgctttcgc cgtcacgctc acgcaattgc tggttcagtt
    20341 gctcggcaag cgttgccagg ctgtgctcca gccgggtgga atcttgcgca ctggcgggga
    20401 ttgcccgata actgtcgagg ctgtcgcttt gtaacagggt cagcgtaccc acgccgacgc
    20461 cgcttgccag cacattgccg tacagtaaat ccgggttaag gcggcttaat gaacgcggca
    20521 gcggatgcgc cgtcagttct gcctgcgtcg gctgaacgct gtcgctgtcg ataaagcgca
    20581 ccaggatgta ctcttccagc acgcgccgcg cctgctcttc atcgctgccg ctaatgttca
    20641 ggctgcaact gtcattaaat aaggtgccag ttccaatcaa cgccagcgag cttttagcat
    20701 cggcctttgc gttctggcga tggttgaaaa aggtgatttc gctttgccac tggctgcact
    20761 gttctttaag ttcccacgcc ggacgagcat gtagaccgtt aggcaaagga cagagaaatt
    20821 gaatcgttaa cataaccgct ccgggttaat caggagatca tctgaaatcc gtcagttgaa
    20881 caaccgtctc acgcgtaaga cgttgaataa gggcagataa aagttgctcc atctgcaaaa
    20941 tgtcgcggca atcggcaatc gatgccgcgc aatgtccgtg gcgggtggca ggccccatta
    21001 ccactgtggg tacgccagta ccggttaagt gcaccgctcc gccgtccgtg ccgccgttgc
    21061 tgaacatatc tgcctgcaac ggtacgccaa tttcagccgc cacggtttcg atccaggcgg
    21121 taagttttgg cggcgcaatc agtgacttgt cgcttaacac cagcatcggg ccgttaccaa
    21181 tctggcgatg gttagccgcg ccataatcaa agtttttcgc ccagcaggcg gtatccagca
    21241 caatggcgac gtccggcgac accgcgcggg tagcggtttg cccaccgcgt aatcccacct
    21301 cttcgctgga acttgccacc agccacacct ccgcaggcag ttcggcatcg tgcagctcac
    21361 gtagtaacgt caccagcaga tagcaaccga ggcgatcatc aaaggctttc cccatcaccc
    21421 gctggtgagg gagaacctga aaagttgtat caaaggtgac gcgatcgcct ggacgaattc
    21481 ccgcctgcat cacttcgtca tacgagcgcg caccaatgtc cacgcgcatg gcgctgacgt
    21541 cattcccctg ccggtcgccg tcaagcaggc ctggaatttt gcactcttca cgggtggtga
    21601 tgcgcaccgg ctgcagctgg cgggcagcca tgcgcacgtt gccaaccggc agcacatcaa
    21661 ttgccccttc gcgggagatg ctacgcacca taaatcccac ttcgtccata tgggcgcaga
    21721 tcatcacctt cggacctgtc gattcattga ggcggatcag caccgatccc agaccatcaa
    21781 atcgcacttc tttttgcagg cgatccgctt cttccagcag gatctgccgc acttcctgtt
    21841 ccgaggaggc gatcgcatct gcctcgctca acgcttttaa tagcgataaa tccattatgc
    21901 ctctcccgtg agcaacactg ttttcggcat ggcgtagagc acttctgcgc cttgcggggt
    21961 aaccagcaca acatcttcga tgcgcacgcc cccttgccct ggcaaataaa tccccggctc
    22021 cacggtcagt aacatgcctg gctgtagcgt cgtggtgtcc cgcggtgaaa aacgcggatc
    22081 ttcatgaact tcaatgccga tagcatgacc ggtgttatga ccgaaatagt ggctaaaacc
    22141 tgcctcggta atcacttggc gcgcggcatc gtcaacctgc tggcagcgca cgccggggcg
    22201 aattgcagag attgctgcga gctgtgcctg caggacaatc tggtagacgt taaacagcgg
    22261 gtgagattcg gcgctcaccc cttcgccatt caccagcaag gtgcgcgtca tatcagagca
    22321 gtagccctga tacagcgcac caaaatcgag agtgacaaac tcgcccgctg caacaatctt
    22381 gtcgctggct ttgccgtgcg gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa
    22441 agaggctttt tctgcgccct gctggcgcat aaaccactcc agttcagcgg ctatctcgcg
    22501 ctcgctcatc cccgcctgaa taaagcggcg aatatgctcc gctccgcgat cggcaatccc
    22561 acaggcgagg cggattatct ccacctcctc tggcgttttg atttgccgca gcacatccgg
    22621 cgtggcgcta accagtttcg cattgagttc agacttccag cggtgcgcgg tttcccaact
    22681 cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga tttgattgac
    22741 gatagtggta agcgtgttcg tcgcgtcaag caaatgcagc tggtagcctt gcgcacgggc
    22801 ttccacctca acgaaatagc gcgaatctac cagaatgtgc gcactttcac ggctaataac
    22861 cacatagcct gatccggtgg agatccccag atgcggctgt ttgttctgcc gtgaggagag
    22921 aagcactgca tccagttgtt gcgccttaag ccagtcgcgc agcgaagcga gtaatgtcat
    22981 ctggattttc cttcttacag gctatcgatt aacaatttgc ctttacggaa catcatcaga
    23041 cgcaggaaca ccaccatcag cgcagttatg actgccccca gcgcgatccc cgccatatag
    23101 acgcccaggt tagtgaccag cggccatgcc cagatagcgg attccgggaa ccattgcact
    23161 gcgcccagcc agacagcggc ggttgagccg acaatcgcgc cgaccatata cgacggaatg
    23221 gcggtgatgg ggctttccag cgcaaacgga atcgcaccct cactaatccc cataaaggca
    23281 aggaacatcg cggtcttacc ctgcggataa agttgagcgt tgaacaggcc tttgccggtt
    23341 aaacgacggt caataatggt cgccagaccc agaccaatcg gaggaataac gatagcgata
    23401 gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg
    23461 gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac
    23521 atcagcgcgc ccttctcacc tgcggcagtc agcacagtac ggataccgcc gttgatccag
    23581 ccgccaaacg gggtgatgac gtagtacatc gccagcatga caaaaatggc ggaaagaatc
    23641 ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat tttctggttc
    23701 atccatttca caaggtaacc ggcaacgata gaaataatca gcgcgccaat gaaggtggac
    23761 ggcaccggcg aagaggtcgc ccattgcata gtgctgggat cgaagttcag cagttgcgtc
    23821 ggctgggtag acatcaagcc gccgataaaa ccagccggga acgccagttt gccaccgata
    23881 gagttcgcca caaaagcagc aaacatcgga atggcaaaac caaacaacac gccgccgaag
    23941 gactgcgaca gccaggcaaa tttcagtaag gagaggtcga aaccggagaa tttcccgcta
    24001 ttaagagcat ccatgatgcc gatctcagca ggaattttaa gccagctata agcaatcagc
    24061 tggctgaaag cgaggatcac accgcccata attaaggtcg gcaccatgcg tgaaatccct
    24121 gacatcacat gctgcgggag ttcaccccag aagctgcttt tagaggcctg cggattctta
    24181 actgccgctg ccgcaccgga tgcgccaggc acaacggttg cactgcgttt tttaatggcc
    24241 ataacaatta tccctgtcga atattactgt tgttcagagg caatcatctc ttcgatttct
    24301 ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt
    24361 gattcgaaac gctcattatc ttccggggta actgccacgg agtggatgat gatggttgct
    24421 tcggcgatat cctgcgccgt caggcgattc tggataccgt ccgcgccctg ggtttcaatt
    24481 ttcacttcat aaccggcttc taccgccgct tcttccagcg cctgagcggc cataaaagtg
    24541 tgagccaggc ccattgggca ggcacataag gcaattagtt tcttactcat cgttaattcc
    24601 tcattcagtg atagttgacg agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag
    24661 acaaataatg catttactgg agaattaatc ccgctgtacg gaagtgtgac gcaggtcggt
    24721 cttgtgaggg gacacaaaga aaaattgaat taactgtatg atttaaaaga ttatcgggag
    24781 agttacctcc cgatataaca ggaaggattt acagaatgtg acctaaggtc tggcgtaaat
    24841 gtgcaccgga accgagaaga cccggattgt catggacgat gagatacacc ggaatatcat
    24901 agacatattc tttaaagcgc cctttatctt caaatgcggc acggaaacca gaggctttga
    24961 agaactcaag gaagcgcggc acgataccgc ccgcaataaa cacgccgcca aatgtcccga
    25021 gattgagcgc cagattgccg ccaaaacggc ccataatgac gcaaaacagc gacaacgcgc
    25081 ggcggcaatc ggtgcagctg tcagccagcg cacgttcggt aatatctttt ggcttgagat
    25141 tttctggcag gcggttgtca gctttcacaa ttgcgcgata caaattcacc agcccagggc
    25201 cagaaagcac gcgttccgcc gaaacatggc caatttccgc acgcagtatt tcgaggataa
    25261 tggcctcttc ttcactattc ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta
    25321 cccaacgctt atcgacatgg accagatgcg caaccccaag ccctgttccg gcaccgtaaa
    25381 ccgcaatagg tttaccttcg accggttctg cgccaccaaa ctgaatcaga tgctcttttt
    25441 tcagcatcgg gatcgccatc gatacagcgg taaaatcgtt aataatttcc agatggctaa
    25501 aaccgagatt ctttttcatt tcggcaattg agaacgccca ggtatggttg gtcattgcca
    25561 cccagtcacc ggtaattggg caagcgatgg caatacagcc gtctttcacc tcgaccttat
    25621 gttcttcaag ataaacgcga atgaccgctt cgaggctggg gtaatcaagc cctgaatagg
    25681 tcttagcctg cgagatttca ccactggcaa tatcacacag agcaagacgt gcgttggtgc
    25741 cgcccacatc accgactaat gcatactttg tcattcttca actgctccgc taaagtcaaa
    25801 ataattcttt ctcacactgt aaatacctgg gggcataaca acaacgccgg aaaggcaggc
    25861 tccctgtaaa tatcgatctg ggtcacacat ttactttatc gtttcagcac caattgcagc
    25921 gatgcctttt tgcaagctgg gcaaactaag tatctgaccc cgcataagga atagaacatg
    25981 ctccatccgc gagccagaac catgttgtta ttatcgctcc ccgccgtggc aattgggatt
    26041 gcgtccagtc ttattctgat tgtggtgatg aaaatcgcct cggtattaca gaatttgctc
    26101 tggcaacgac tgccgggaac tctggggata gcccaggatt cacctctctg gatcatcggt
    26161 gtattaacgc taacgggtat tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc
    26221 ggaccagacc ccgcctgtga accgctgatc ggcgcaccag ttccgccctc tgcgctacca
    26281 ggacttatcg tagcattaat tctcggtctt gctggcggcg tcagcctggg gccggaacat
    26341 ccgatcatga ccgtcaatat cgcccttgcg gttgccattg gcgctcgtct gttaccgcgc
    26401 gtcaaccgaa tggagtggac tattttagcc tctgccggaa ccatcggcgc gctgtttggt
    26461 acacctgttg cggcggcgtt gatattttcg caaaccttaa atggcagtag tgaagttccg
    26521 ctatgggatc gtctttttgc gccattaatg gcggcagcag ctggtgcact tactaccgga
    26581 ttatttttcc atccccattt ttcactgccc attgcgcatt acggacagat ggaaatgacc
    26641 gatattctca gcggtgcaat tgtcgcggcg attgccatcg cagcaggaat ggtcgccgta
    26701 tggtgcttac cacggttgca cgcgatgatg catcaaatga aaaatccggt gctcgtgcta
    26761 ggtattggcg gatttattct cggtattctg ggagttattg gtgggccggt ttcgctgttt
    26821 aaagggctgg atgagatgca gcagatggtg gcaaatcagg ctttcagcac cagcgattac
    26881 tttttgctgg cggtaattaa acttgccgcc ctggtcgttg ctgccgccag tggctttcgc
    26941 ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat tagggttgat gctgcatgag
    27001 cacgttcccg ccgttccagc ggcaataacc gtttcctgcg ctattctcgg catcgtgctg
    27061 gtggtaacac gcgatggctg gttaagtctt tttatggcgg cagtcgttgt acccaatacc
    27121 acattgctac cgctgctctg tatcgtcatg cttccggcat ggctgttatt agcaggtaag
    27181 ccgatgatga tggtcaatcg tccgaagcaa cagccacccc acgataatgt ttagcaaaaa
    27241 atgctcttct ttatgattta agagttatgg cgatgatacg gggctttacc tccccgtaat
    27301 attgcgttaa caggccgctg acggcttcga tcgtgaagga gaataacaat gttcaggtca
    27361 ctgtttctgg cggccgccct gatggcattt accccgcttg cagcaaatgc aggtgaaatc
    27421 accctactgc catcgatcaa attacaaatt ggcgatcgcg atcattacgg taattactgg
    27481 gacggtggtc actggcgaga tcgtgattac tggcatcgta attatgaatg gcgcaaaaac
    27541 cgctggtggc gtcatgataa tggctatcat cgtggctggg ataagcgtaa agcatatgag
    27601 cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat
    27661 gggcaccgcc attaaacgcg tcctccaatg gagcacaatg cctgatgcga cgctagcgcg
    27721 tcttatcagg cctacaaacc ggctctttaa cgcgtagggc ggataaggcg tttacgccgc
    27781 atccgccaat agtgccagat gcgacgctca ttcaactaca atcccagcgc cgtccccacc
    27841 aacaaccaga tatttagcgc cacgaccagc actacaatca cccagcctgt ctgttttacg
    27901 cgtttgctgt tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga
    27961 accagcgcca gggcgatacc aaaacttaac agcacctgac tcataaccag aatccgtgtc
    28021 ggatctaatc ccatcagaat gacaataaat gacggcagca tggtgactgt acgacgcacc
    28081 cacagcggga tatggaagcg aatgaacccc tgcatcacca cctgccctgc cagtgtcccc
    28141 accaccgttg aggacagtcc ggcagcaacc aggcttaatc caaagaccgt tgccgcagca
    28201 tggcttaaca acggttgcag cgtcagataa gcctcatcaa gatcggcaac accagtatga
    28261 ccagaaaagt ggaacgccgc cgcagctgta gccatcatcg ccagattgac aaaaccggca
    28321 atcgtcatgg cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaaccg
    28381 ccatgtaaat gctgagtgag cgaagagtgc aaataaatca catgcggcat aatcgtcgcc
    28441 cctaacacgc ctgctgccag gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt
    28501 cctttaccca gctgcgccag gttaggctgg gagaaaatca actcgacaat gtaagccgcg
    28561 gcaacaaaca acagtaaccc gccaatcact ttctccagcg gtttttgccc gcgacgttgc
    28621 agcattaaaa tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca
    28681 ccaagaatga gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc
    28741 gcaataattt ctgcctgaac ccaatagaac cacactacgg gacgcggata gtgatcacga
    28801 atctgctccg ccagattttt accggtggca atccctagtt tggcagagag gatctgaatc
    28861 agcatcgcca tcaggttggc ccaaacgaca acccacagta gctgatagcc gaagctagca
    28921 cccgcctgaa tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac
    28981 gcaggtccca ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct actctcaacg
    29041 cgatagttcg tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctatgcca
    29101 aacgagaatg attatcaaat tcatttgaat gggttgtggt gatttctttg atagaccagg
    29161 attatgacta cgaaaagatt gatgacttga atgttatggt tatgtttaat gttagcacat
    29221 ttacataact ttcagcttcc atacacaaca tagcagaaat gtatgacaga tcactatttt
    29281 tgaagcttgt cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt
    29341 tacatagaat gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgcgt
    29401 ccttcatttt gtactggcac ttgccgttgt tgcgattctc gcactgctgg taagcagcga
    29461 ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc
    29521 gtggttcttc ctgaactccg acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt
    29581 cgaaaaactg ctcggatttg ccaacgaagg gactaacttc gtctttggta gcatgaatga
    29641 tcaaggcctg gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcact
    29701 gatcggtatt ctccagcaca ttcgcgtgtt gccggtgatc atccgcgcaa ttggtttcct
    29761 gctctccaaa gtcaacggca tgggcaaact ggaatccttt aacgccgtca gctccctgat
    29821 tctgggtcag tctgaaaact ttattgccta taaagatatc ctcggcaaaa tctcccgtaa
    29881 tcgtatgtac accatggctg ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc
    29941 atacatgacc atgctggaac cgaaatacgt cgttgctgcg ctggtactga acatgttcag
    30001 cacctttatc gtgctgtcgc tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat
    30061 ccagatgtcc aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct
    30121 ggcaggtttc aaagttgcca ttatcgttgc cgcgatgctg atcggcttta tcgccctgat
    30181 cgccgcgctg aacgcactgt ttgccaccgt tactggctgg tttggctaca gcatctcctt
    30241 ccagggcatc ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag
    30301 tgaagcactg caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc
    30361 gatgatggat ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag gcatcatctc
    30421 tgtgttcctg gtttccttcg ctaacttctc ttcaatcggg attatcgcag gtgcagttaa
    30481 aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc ggtctgaagc tggtttacgg
    30541 ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt aagaccatac
    30601 ataaaaaagc cggggataat tccccggttt ttttacgcct gttaatcagc taatggctgc
    30661 gggcgaccaa tcaaataccc ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc
    30721 tgctgttgcg tctcgacaaa ctccgcgacc acactcaatg acttcgcttt cgccagatcg
    30781 gtaattgatc tcacaatcat cgcatccagc gtattcgtga caatatcttt cacaaagacg
    30841 ccatcaattt tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg
    30901 gtgccaaagt catcaatcgc aatccggaaa ccaaacttat gcagctgctc gatgttgtac
    30961 atgctgcttt ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc
    31021 ggggagatgt gataacgctt aaacagacga ataatccgcc cggcaatatt cttttgcagc
    31081 agcgtgagcg gcattaaatt gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt
    31141 gccagccact tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac
    31201 tgagcaataa ggggcagaaa cttatccggg gtcataatgc cgccgtcata tttcagtcgc
    31261 gcgaggatct catcataacc gtcaccctct ttgttgcgaa ttggctgggc gtagagcagc
    31321 aaatcaccct gatctaacgc cgtgcgaatg gtattcagca atagcacctg tttagtggtc
    31381 tgcccggaaa ccatctcctc tctgctatcc agcgccagca catgatgatg tgcgcaggat
    31441 tgctccgcca gccagcttaa ctgccccaac aagggttgca gggtttcctg atttccatca
    31501 aaacgccccc aggcagcacc atagcccata tccagcccgg tattgttcca gtgaatttgc
    31561 cgactattca ggatgttaac catatgctgg agtcgccctt ccgtttccgg cccgctcagc
    31621 actaacagca gttcactacc cggcaattga tacaactttt cgttttcctg catcaacggc
    31681 agcagcgtac ggcaaattga gcggatacag tgaacgcgca tcattaagcc gtaatgacga
    31741 ctcataaact caagattatc aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc
    31801 ggcgcttgtt ccaacgcacg aaagttgggt aggagcgtta acggatctgt cagcgcctgc
    31861 aaatgccagc ggcgattaag ccattcactg cgatgataaa tgcgcaccat atagagcagg
    31921 caaacgctaa aggaaatcag caccgcaaga ataaatgcca gcgaatattc ggtttccacc
    31981 ccttgcaaaa agttctggtt gtaattcagc aggcaaagcg ttgaaacagc ccaggtgaga
    32041 tttaaaaacg gatagcgaag cttaccgacc ccgagggtaa agatgatgaa gaaaacgggt
    32101 accaagtaac cggcaataaa gtcgttttca taaggtgtgc acaacagaag tagcagcacg
    32161 cttaacgctg ccagccagct taaggtaaat gcgcgtttct ctttgcccaa cgacggagcg
    32221 atatccctgc gccacaatat ctgcgcaaag tggggactta caatcatgcg agtgagatag
    32281 tagaaaagca tgttgtaaat cagcacagcg gtgaaaaggc ttagcaaatc aacgaccgtg
    32341 aaaatggcat ccgcatcgcc gaaaaaggtg gatatcttta gcgggaaatc aaagaagctc
    32401 cccacaagat acatgctgca tttgatgccg atcggcgtca ccaaaccaag ccagaacaga
    32461 cgttgccaga catatcggct ggtcaatcca taacgccaac gcgtccccag ctgccagcgt
    32521 aatattgcgc aggcacacaa cacgacaaac gtctggctac cgagcatgac cgcagtctgt
    32581 aaaaaagaga gatgaaagtt ccacagattg gtgcaaaaca tcccgcataa aacgggaacg
    32641 acaccgcgcc agccaaagat aaacaagatt gacagcatta cgcacagtgg catccacgcc
    32701 agaaagatat aactggaatg gataatggca agtggcgaaa taaaacggga tagctgaata
    32761 agtaccacgg tgagcgtaaa cgctagtgtg aatatcttga tattttttat caggttatgc
    32821 tccacaaaca tgagtacact actctcactg atcggggttt tcaggcacac aaataataag
    32881 tttattcgca tcgcgaagca aacaggggcc agcctgttgc cgatttttca tccaatgatg
    32941 ctttcaccag gcggcgctaa cccggacgta gactgtatta caaaagcggc aaaaagcaga
    33001 gacaaaaaac ccccgctttg cagcgagggt tggaaatttg gtggagctaa gcgggatcga
    33061 accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccacgatg
    33121 cgtttacgta ccaaatttgc tgggtgcaaa atttggtgga gctaagcggg atcgaaccgc
    33181 tgacctcttg catgccatgc aagcgctctc ccagctgagc tatagccccg tcacgtaaag
    33241 cttgtcgagt tgacgggcgg catcatatga attccgcccg aatgtgtcaa cggcaaattg
    33301 caacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga aaaccgctcg
    33361 cattcagtag ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa tgaactacta
    33421 atagacccac atacattcag ggaattgtta tgttcaagga gaggatgaca ccagatgaac
    33481 ttgccagact gaccggctat agccgccaga ccattaataa atgggtacgc aaggaaggct
    33541 ggacaacgtc accaaaacct ggcgtccagg gtggcaaagc ccgactggtt cacgtcaatg
    33601 aacaggttcg tgaatatatt cgcaatgctg aacgtccaga aggtcaggga gaagcgcctg
    33661 ccctttctgg tgatgcgccg cttgaagtat tgctggtgac actggcgaaa gagatgacgc
    33721 cagttgaaca aaaacagttt acatccttgc ttctgcggga agggattatc ggattgttac
    33781 aacgcttagg gattcgcgat agcaaataat atgaaaagat tacgcaacaa aatgaccacc
    33841 gaagaactgg ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg gatcagagaa
    33901 aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag acttattctg
    33961 gtcgatacgc aagtttgcga gtttattcag aacacgccag ccttccataa cacaccaatg
    34021 ttgatggaag ctgaagagag aattgctgaa tacgctcctg gagcacgcgc cccggcgtat
    34081 cgacaaatca tcaacgcgat agataatatg acagacattg agcaggaaaa agtcgcgcaa
    34141 tttttgtcac gtgaaggaat tcgcaatttc ctcgcccgtc tcgatattga cgaatcagca
    34201 taaataaaaa acggcaggat attatctcct gccgtttatc tttttacacg ctaattactg
    34261 ctgattttcg cgttcagcaa taaaatccag cgctttgttg atacgctcga tactgcgggt
    34321 cttaccaatt gcgtgaacgg taacatccag tgctggagac tgccccgcac cggttacggc
    34381 gacacgcagc ggcataccaa ctttacccat acccacttcc agctcatccg ccgtcgcctg
    34441 aatagcgtga tgaacgtttt cagcggtcca gtcagtaatc gcggccagtt tgtcacgaac
    34501 cacttccagc ggctgacgcg ctaccggacg cagatgtttt ttcgccgcat cggcatcgaa
    34561 ctcagcaaaa tcttcgtaga aataacggca gctctgtgcc atctctttca gcgtcttgca
    34621 gcgttcgccc agcagtttca ccagatcagc cagctgcggg ccgttacggg tatcgatatt
    34681 ttcctgctca atgtgccact gtaagtgagt agcaacatac tccggcggca gcgcgttaat
    34741 gtagtgatgg ttcagccaca gcagcttgtc ggtgttgaac gcactggcag atttgctgac
    34801 ggcattcaaa gtgaagtatt tgatcatctc ttcacgagtg aagatttcct gatcgccgtg
    34861 ggaccagccc agacgcacca gatagttcag cagtgcttct ggcaaataac cgtcatcacg
    34921 atactgcatt acgctgactg ccccgtgacg tttggacagt tttttaccgt catcgccatt
    34981 gatcatagaa acgtgcgcgt aaaccggcac cggcgctttc agggccttaa gaatgttgat
    35041 ctggcgcggc gtgttgttga tatggtcttc gccacggata acgtgggtga tttccatatc
    35101 ccagtcatcg acaaccacac agaagttata ggttggggaa ccatcggtac ggcggataat
    35161 cagatcatcc agctcctggt tgctgaactc gatcggacca cggatctgat cgtcaaaaac
    35221 aacagaacct tcctgcggat tagcaaaacg cacgacacac ggttcatcat cagcatgatg
    35281 ctcatggctg tggcggcagc gaccgtcata acgcggcttc tcacctttcg ccatttgctc
    35341 ttcgcgcagc gcttccaggc gctctttaga gcaatagcat ttataagcag tgccctcttc
    35401 cagcatctga tcgatcaccg cattgtagcg atcaaaacgt ttggtctggt agtacggacc
    35461 ttcatcccac tccaggctca gccagttcat gccatccata atggcttcga tagcttccgg
    35521 cgtggaacgc tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc cgtggttacg
    35581 tgcaaaaagc caggagtaaa gagcagtacg cgcgccgcca acgtgcagat agcctgttgg
    35641 gcttggcgcg aagcgagttt tgattttcat gaaatggcct tacgtttaga aagatgccga
    35701 caaccggcaa atcctggaaa aattgagtgg gcgatattct atcactcccg cctgattcct
    35761 caatgtagtt cgggttgcaa ccttacgcat tattgttttc gttgacaaat tgcgcaacac
    35821 ggtctgtttt gcgatcatct tgcttaaatt tacgacgaac gaacaatttc tttagaaaat
    35881 gcgttgactc attttgaact ctccctataa tgcgactcca cacagcgggg gtgattagct
    35941 cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc
    36001 accaactact ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg gtgattagct
    36061 cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc
    36121 accacttcgg gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag
    36181 gttcgaatcc tgcacgaccc accaatgtaa aaaaagcgcc ctaaaggcgc ttttttgcta
    36241 tctgcgata
//
LOCUS       DAZFJF010000036        35358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFJF010000036 DAZFJF010000000
VERSION     DAZFJF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.036"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHKAU_001984"
     CDS             complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHKAU_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO5531050.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(580..798)
                     /gene="yiiE"
                     /locus_tag="ACHKAU_001985"
     CDS             complement(580..798)
                     /gene="yiiE"
                     /locus_tag="ACHKAU_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5531051.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(1403..1693)
                     /gene="nadS"
                     /locus_tag="ACHKAU_001986"
     CDS             complement(1403..1693)
                     /gene="nadS"
                     /locus_tag="ACHKAU_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5531052.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1694..2005)
                     /locus_tag="ACHKAU_001987"
     CDS             complement(1694..2005)
                     /locus_tag="ACHKAU_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5531053.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            2234..3142
                     /locus_tag="ACHKAU_001988"
     CDS             2234..3142
                     /locus_tag="ACHKAU_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5531054.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(3206..4147)
                     /gene="fabY"
                     /locus_tag="ACHKAU_001989"
                     /gene_synonym="yiiD"
     CDS             complement(3206..4147)
                     /gene="fabY"
                     /locus_tag="ACHKAU_001989"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO5531055.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(4192..4629)
                     /gene="dtd"
                     /locus_tag="ACHKAU_001990"
     CDS             complement(4192..4629)
                     /gene="dtd"
                     /locus_tag="ACHKAU_001990"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO5531056.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4626..5498)
                     /gene="yihY"
                     /locus_tag="ACHKAU_001991"
     CDS             complement(4626..5498)
                     /gene="yihY"
                     /locus_tag="ACHKAU_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO5531057.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(5492..6091)
                     /gene="yihX"
                     /locus_tag="ACHKAU_001992"
     CDS             complement(5492..6091)
                     /gene="yihX"
                     /locus_tag="ACHKAU_001992"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO5531058.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(6190..6975)
                     /gene="csqR"
                     /locus_tag="ACHKAU_001993"
     CDS             complement(6190..6975)
                     /gene="csqR"
                     /locus_tag="ACHKAU_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5531059.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(7009..7905)
                     /gene="yihV"
                     /locus_tag="ACHKAU_001994"
     CDS             complement(7009..7905)
                     /gene="yihV"
                     /locus_tag="ACHKAU_001994"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO5531060.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            8073..8969
                     /gene="yihU"
                     /locus_tag="ACHKAU_001995"
     CDS             8073..8969
                     /gene="yihU"
                     /locus_tag="ACHKAU_001995"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO5531061.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8993..9871
                     /gene="yihT"
                     /locus_tag="ACHKAU_001996"
     CDS             8993..9871
                     /gene="yihT"
                     /locus_tag="ACHKAU_001996"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO5531062.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            9888..11129
                     /gene="yihS"
                     /locus_tag="ACHKAU_001997"
     CDS             9888..11129
                     /gene="yihS"
                     /locus_tag="ACHKAU_001997"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO5531063.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            11267..12169
                     /gene="yihR"
                     /locus_tag="ACHKAU_001998"
     CDS             11267..12169
                     /gene="yihR"
                     /locus_tag="ACHKAU_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO5531064.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            12368..14404
                     /gene="yihQ"
                     /locus_tag="ACHKAU_001999"
     CDS             12368..14404
                     /gene="yihQ"
                     /locus_tag="ACHKAU_001999"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO5531065.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            14450..15835
                     /gene="yihP"
                     /locus_tag="ACHKAU_002000"
     CDS             14450..15835
                     /gene="yihP"
                     /locus_tag="ACHKAU_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5531066.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15878..17281
                     /gene="yihO"
                     /locus_tag="ACHKAU_002001"
     CDS             15878..17281
                     /gene="yihO"
                     /locus_tag="ACHKAU_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5531067.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            17349..18041
                     /gene="ompL"
                     /locus_tag="ACHKAU_002002"
     CDS             17349..18041
                     /gene="ompL"
                     /locus_tag="ACHKAU_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO5531068.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(18132..19397)
                     /gene="yihN"
                     /locus_tag="ACHKAU_002003"
     CDS             complement(18132..19397)
                     /gene="yihN"
                     /locus_tag="ACHKAU_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5531069.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(19499..20479)
                     /gene="yihM"
                     /locus_tag="ACHKAU_002004"
     CDS             complement(19499..20479)
                     /gene="yihM"
                     /locus_tag="ACHKAU_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5531070.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(20487..21197)
                     /gene="yihL"
                     /locus_tag="ACHKAU_002005"
     CDS             complement(20487..21197)
                     /gene="yihL"
                     /locus_tag="ACHKAU_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5531071.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(21414..23237)
                     /gene="typA"
                     /locus_tag="ACHKAU_002006"
     CDS             complement(21414..23237)
                     /gene="typA"
                     /locus_tag="ACHKAU_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO5531072.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            23610..25019
                     /gene="glnA"
                     /locus_tag="ACHKAU_002007"
     CDS             23610..25019
                     /gene="glnA"
                     /locus_tag="ACHKAU_002007"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO5531073.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            25338..26387
                     /gene="glnL"
                     /locus_tag="ACHKAU_002008"
     CDS             25338..26387
                     /gene="glnL"
                     /locus_tag="ACHKAU_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO5531074.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            26399..27808
                     /gene="glnG"
                     /locus_tag="ACHKAU_002009"
     CDS             26399..27808
                     /gene="glnG"
                     /locus_tag="ACHKAU_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO5531075.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            27920..28030
                     /gene="yshB"
                     /locus_tag="ACHKAU_002010"
     CDS             27920..28030
                     /gene="yshB"
                     /locus_tag="ACHKAU_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO5531076.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(28220..29593)
                     /gene="hemN"
                     /locus_tag="ACHKAU_002011"
     CDS             complement(28220..29593)
                     /gene="hemN"
                     /locus_tag="ACHKAU_002011"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO5531077.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(29782..30291)
                     /gene="yihI"
                     /locus_tag="ACHKAU_002012"
     CDS             complement(29782..30291)
                     /gene="yihI"
                     /locus_tag="ACHKAU_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO5531078.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(30410..30576)
                     /locus_tag="ACHKAU_002013"
                     /pseudo
     CDS             complement(30410..30576)
                     /locus_tag="ACHKAU_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30873..31505
                     /gene="yihA"
                     /locus_tag="ACHKAU_002014"
     CDS             30873..31505
                     /gene="yihA"
                     /locus_tag="ACHKAU_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO5531079.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(31887..34673)
                     /gene="polA"
                     /locus_tag="ACHKAU_002015"
     CDS             complement(31887..34673)
                     /gene="polA"
                     /locus_tag="ACHKAU_002015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO5531080.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            35037..>35358
                     /locus_tag="ACHKAU_002016"
     CDS             35037..>35358
                     /locus_tag="ACHKAU_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5531081.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWA"
BASE COUNT         8627 a         9071 c         8631 g         9029 t
ORIGIN      
        1 aataaacttc aatatattga atttgcatga ctttgtaggc cggataaggc gttcacgccg
       61 catccggcat gaacaacgcg cacgttgtca gcaatctgat tgacatcatt ttttcccatt
      121 accattcctc gcgcagctta cgctgatatt cgacaccatc ttcctcacac ccttgccaga
      181 tgccaggaac actggttctt gctgtttgcg attgatttat caggtattga tctaccgctt
      241 cccttaatag gtccgcacga ggaagattac gctgcacctc gagatcatca agttgcttaa
      301 tcacctcatt cgataaattg agtaaaattc tgcccatatc tatacctgcc agagagttca
      361 taaatatctc gccaatatca tttttgaatc tatggagaga aaagtaccct tgccgaatct
      421 ttaaagaaag ctcatttacg catcattttt tatttttgcg aggcgttttc cagatacgat
      481 gtaacagttg cagttattat aagttcaagt aaaacagcct tgtattaata ccagaataag
      541 ggcatctgtt tttttatatt caagaatgaa aaattttttt cattccttat gctccttaca
      601 ctgatcatca acaccaacta cctccccctt ttgcctgcct ccctttgctt tacgcaccgg
      661 aaattgctgc cgctccagat attgctcaac cgcttcgcgt aaaattgcag atcgtgaaac
      721 cttacgccag ccccgaagtt tgttcagccg tttaattgcc tcttctgata aatgaagaaa
      781 aaccgtattc atcgccatta ccaccacctt acctatatta ctcattaatg tatgtgcgaa
      841 aattacgccg gaaaatcatt taaaaaaagc ggatttttgc atgttgtttt gtttttgcga
      901 ggcgttttcc agaaataata agagtgttgc agttgtttca tttattactc aaaaatggtt
      961 tagttatcgc ccaggatgag ttatggatgc tatgaatata ttatattcag ggatgtttat
     1021 tttttgattg gatctttatt tgcataatct gaatggtgta cccgaaaaat gtcagcatcc
     1081 taacagcacc cggatttaag gtgaaattaa tcatccatca cagaccggtc agtactgcca
     1141 ccaaccggtc tttacttgtt catcgttata attttgttgg taacagttcg ggaaacaagg
     1201 aattccttcc ggaacccccc ggtatcatcc gatagcattg acgtcaatca tcagcgcgac
     1261 gatgagtttt tttaatcgca tcatagggtg gcgacgttca gcacgattac gggtcatagc
     1321 aaaactcctg ggcaggcgac cactatgccg ctttcacagg agttctgaat gctagtgctt
     1381 tctgtatggc ataaccatgc ctctaacggt taagtgcttg cagcacatgt tgcggatcat
     1441 tggcaatcgc tcgcagcaaa gcctttgcag gcccggtcgg tgaacgtcgc ccctgctccc
     1501 agttacgcaa cgtgtctacg cttacagaaa taagatcggc aaatttagat tgtgacaacc
     1561 ctgaagcgcg gcgtatttct ttgattttca tagcatcaac ttcaaaagtc cgcgaaggct
     1621 gtcgcgttcc ttcaataatt tcattcatct gggccatgct gtgagtcaac cgctcaaaaa
     1681 gtgccttatc catttaccac ctcgtattta acaaccggag cattgctttt tcctggggag
     1741 acagatcatc tttaataccc ttacgataaa taagcagtaa tctgatctca taatgtttaa
     1801 cctgatgaaa gtaaataacc ctgacaccag cacgtttccc ctttcctcct gacacccatc
     1861 gaacctttct taacccacca gtttcaggta ttacctcacc atagtcagga ttcaaggcca
     1921 ggaaaaactg aaaacgactg aattcatcgt cggtcagcag tttttgaaca tcttcagtaa
     1981 aaatttccgt ttcgatgaag agcataatta tacgccattg gcttagagta acaatccttt
     2041 ttgtacgtca ttgacgtacc cttgaaacca ctagcttact gatagtgctc cagcaaaacc
     2101 acgtctctgc cgggtttgtc tttagatttg cgaggcgctt tccagatttt tcacgcccgt
     2161 gaatttccct gtatccatcc agcaaaaacg ggtgtataag actgtacatt catacagcaa
     2221 aataagttct actatggcac tggaaaaagg aattgcagag ttagtcgaaa agtttatcgc
     2281 tgcgggtcgc ccctcatcgc gtcaacaaag tattgcgcag cgcagggagg gctatatcgc
     2341 cagcgctgtc cttgcgggtg agacagaaac gcgggtagat attcagacta tcgaacttga
     2401 agggatgacc ttacgaatag tgtcgccgct gaatgccccg acattactcc cgacgataat
     2461 ttattactac ggtggctgtt ttgttagcgg cgggttcgca actcacgaca accagctgcg
     2521 ccagttagcc tattacgggc aatgtcgggt tattgccgtc cagtatcggc tggcaccgga
     2581 acatactttc cccgctgccc atgatgatgc gcaacgaggt gcagaaatag tctggcaaca
     2641 cgccgaacga ctcggcgttg ataaacaacg tataactctt gccggtgaca gtgcgggcgg
     2701 acatctggcg ctggtaactg ctttgcgatt aaagcgtgct ggcgagtggc aaccggcaca
     2761 actcatctta atttatccta tgcttgatgc gactgcgcat ttcgaaagct acatacgcaa
     2821 tggtcacgat tacatcatca cacgcgacac tttactgagc ggctttgaga tgtatctgcc
     2881 aggcattgaa cgcaggcatc ctgaagccag ccctctttgg cgcgatgatt tcaatggctt
     2941 gccacctgta catatcatta ccgctgaata cgacccactt tgcgatgaag gtgaagcgtt
     3001 gtatcaccgc atgacagggc aaggagtgca atgcacctgc caacgctacc ttggcgttat
     3061 ccacggattt tttcagttag gtggtatcag tgaggccgcg cgatcggcac tacgggatgt
     3121 tgcctggaga gcgggaaggt aacttcggac ctgggccctg aaaaggtttt cagattgaaa
     3181 accttttcag tcaagtaaaa cgcccctact cttcttcgtt cccgccctct tcatacgggc
     3241 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
     3301 cgccaaagat ttcaacttgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga
     3361 gatcgccgct taaggcacca aggtcggcta ccgcatgagg tttaccgcta atcggcttgc
     3421 tgtagcggat atgcgcatcc gccagaataa tcgttccgcc gagatggcgt tcgcgcagca
     3481 tcagccagat aagcccccaa ccggtgagcg ttgccagtga gaataaactc ccggcaaaca
     3541 gcgtatggtg cggattctga ttaccggttt ccggcatggt ggtgataaat ttttgcccgg
     3601 tatattgttg aatacgcacg cccatttttt cactaagcgg gatatgttcg taccacgcct
     3661 gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcgacgggct
     3721 taatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa
     3781 caaaccccag cttggcgaaa aactccaccg cgtcttcacg ggcgctacag gtcacgcgct
     3841 taacgccttc ctgacgcgcc accgactcca gggtcatcgc catcagcgtt cctaaccctt
     3901 tgtcctgcac gtccgggtga acggccataa agcgaatgga cgcttcattg tcggcattaa
     3961 tatacaatcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
     4021 ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca
     4081 tttcccagcg aaactgatag taacgctcta actcttcttc tgtttgtgga acccgaaggt
     4141 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     4201 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatcgg cagcgaagcg
     4261 tcctgtttgc gtgttcatct cttgctggcg gcagcgttcg acgaaataat catataacgc
     4321 ctctgcgcga tccggtgatg cgcctttaga gaaacttggg cgcatccccc gttcggtatc
     4381 tgcggcgagg gtaaactggg aaaccaccag tacactgccg cccgcctgtt gcacgttgag
     4441 attcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg
     4501 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc
     4561 aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     4621 tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc
     4681 ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa
     4741 acaaagagaa tggggatcac cgccaacacg ccgtaaatga gctgatatga cgggaacatg
     4801 gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagcgcggc gacaaacgcg
     4861 ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag
     4921 aaggagatcc acgacaacag cagcggaaaa atacgcagca cgttatcgat gacagtattg
     4981 agatcgctcg cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc
     5041 agcagcggcc ccagcgttaa aatcatccag tacacggcga acgagtaaat ttttggtcgc
     5101 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat cagcaataac
     5161 gcggtgacga tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat
     5221 tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     5281 tgacgtaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     5341 acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt
     5401 gtcatgttgt cctcatcaat acgttgccag agtagtttta gccaggccca tagtggacgg
     5461 gtacggtgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     5521 ggatggtggt tttatctttc accagaatac tggtaatgcc cagctgattg gctccttcta
     5581 tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca
     5641 aaacatgctg gtaaattcgt gcttcaggtt tgcgcatccc cagatcttgc gacagataga
     5701 tatggtcagc agcatcacga atttccgggt attcttccgg ccagaaggtg gtatgcaggc
     5761 ggttagtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg
     5821 cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt
     5881 agcttagcgg cagggccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt
     5941 ccccacgctc atgctgatga aacgcctccc ccatgtggaa actcttctta agcgtagcca
     6001 gtggaacacg cgttaaatcg ctccaggctc ccagcacacg gttaaagtcg atatcgacaa
     6061 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     6121 aactgtagcg ggaaaggatg ggtttggcta tgtggcaagg tatgaagcca ggttaacggt
     6181 cacaaaaatc tacgcgtctt cctggggaag aatgatttca atttggctgt cttgtaagcg
     6241 tgattttagc gcagcgggcg gcagctcgtc ggtaaagagc gcagtgacct gtgccacgtt
     6301 accaatttca accgccgccg aagcatgata cttagtgtga tcggcgacca gcagaatatt
     6361 tctcgcgtgc gccatcatcg ttttcaccac gttagcttcg tttacatcaa actccatcaa
     6421 cgcgccatcg ctctcaatcg ccccaacgct tgttaccaga taatcagcac gaaaatcagc
     6481 cacaaaggac gccgctgaag ggccaatgat cccgctatta tgagagcgca acgtaccgcc
     6541 gggcaccatc acttcaaagc gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt
     6601 ggtgattatc cgcaaatgat tatggttaag taacgcccgg gcaacatgct caacagtcgt
     6661 accaatggtg ataaatattg ttgaaccatc aggaatatag tctgccaccg cttcggcaat
     6721 cgcttttttt tcctcggttt gcgaaacctc acgctgctcg aacgccgtat taacgacgct
     6781 ggaagcccga cccgcgccac cgtgatggcg cgtaatcagg ccttgctcgc ttaatttacg
     6841 aatatcccgg cggaccgtct gcgtggagac atccagcaga tttgccagct catcaatatt
     6901 catataccca cgctcggcga tcagcatgag gagttggtcg tgccgcgggt taccggttag
     6961 ttcggtaagg ctcatgaaaa ttcctcgaaa aaccatcacc ctggcatttt atacaaaaag
     7021 tgacaaaaaa gatcgggttt gatcacagtc agggatcccg gctcgtccac cgggacgtgt
     7081 gcattttaac gccgctacac cgctggcgaa gcggactgac tccgctaaat ccccacttgt
     7141 tgccagcgcc accgccaaag cgccgtgaaa aacatcaccc gcacctgtgg tatctaccac
     7201 atcaactttg aaggccggtt gatgctgacg cccaccattt tccagccagt cgcagcctgc
     7261 gctaccctgg gtcacataga catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact
     7321 ggccatctct ttcacgcccg ttaagcgcgc cagacccggt tctgaaaagg ccgcgtgatc
     7381 gcttaatgcc accagctcac tgatatcctg cggcgtaatg tccccgtcca gaacggtcat
     7441 cacacccgcc tgacgggcca gggtgaaggc ttttttagcg ccgtcgtgcc agcgtacatc
     7501 tgccagcaca acatcccact gagagaaatc aatttcctcc aaccactctg cgtcaggcag
     7561 cagatccggg ctggggtagt taatgattat ccgctcgcct ttggtatcca ccatgatggc
     7621 ggattgcgaa gatttcgcct ggttataccg tttggtgtaa cgggtgttaa cgccccagga
     7681 ttccagctct gccagcaggc tgttgcccgt gtcgtcatca cctacgcgac caataaaatc
     7741 gacctgcgcc cccagccttg ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac
     7801 ttccgtataa tttctcgcca cgtatttacc gctctccgtc ggtaaccctt ccacgtaata
     7861 gatgcgatcc atcacggtta tacctacaca agcaacacga atcatggtca ttccttaaac
     7921 attttgggat gcgttcattt taatttcacc aattgtttaa aaagtgacgg ctgtcaattt
     7981 tttgacgata aattacaaat acgatcaaaa acagacaagc aaagcatgtc ataaatgaca
     8041 aaaagtgaca tcactgtatt caggagaggg ttatggcagc aatcgcgttt atcggtttag
     8101 gacaaatggg ttcgccaatg gcgagcaatt tattgcagca agggcatcaa cttcgcgtct
     8161 ttgatgtgaa cgccgaagct gtgcggcatc tggtagacaa aggtgcgact cccgccgcca
     8221 acccggcgca ggccgctaaa gatgccgaat ttatcattac catgttgccg aatggcgatc
     8281 tggtgcgcaa cgtgttgttc ggtgaaaacg gcgtttgcga aggcttatct accgatgcgc
     8341 tggtcattga tatgtccacc atccatccgc tgcaaaccga taaattgatt gccgatatgc
     8401 aagccaaagg cttcagcatg atggatgttc cggtaggccg tacttctgca aatgccatta
     8461 ccggtactct gttactgctg gctggcggca ccgctgaaca agttgaacgt gccacaccga
     8521 tcctgatggc gatgggcagt gagttgatca acgctggcgg tccgggcatg gggatccgcg
     8581 ttaagctcat caacaactac atgagcatcg cgctcaatgc gctttcggca gaagccgccg
     8641 ttttgtgcga agccctgaat cttcccttcg atgttgccgt caaagtgatg agcggtaccg
     8701 ccgccggtaa aggtcacttc accacttcct ggccaaacaa agtcctcagc ggagatcttt
     8761 ctcccgcctt catgatcgat cttgcccata aggatcttgg catcgccctt gatgtcgcca
     8821 accagctgca tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat agccaggcgc
     8881 gcgcagcggg tcgcggtcgc caggactggt ccgccattct ggaacaggtc cgtgtcagtg
     8941 ccgggatgac tgccaaagta aaaatgtaac gactggataa aggaataaat gaatgaataa
     9001 gtacaccatc aacgacatta cgcgcgcatc gggcggtttt gccatgctgg cggtcgatca
     9061 gcgcgaagcc atgcgcatga tgtttgccgc ggctggggca cccgctccgg tagccgatag
     9121 cgttttaact gatttcaaag ttaacgctgc aaaggccctc tcgccttatg cctcggcgat
     9181 tctggtagat caacaattct gctaccgcca ggtggttgag caaaacgcga ttgccaaaag
     9241 ttgcgccatg attgtcgccg ccgatgagtt cattcctggc aacggtattc cggtcgatag
     9301 cgtggttatt gaccgcaaaa tcaatccgct acagatcaaa caagacggtg gcaaagcctt
     9361 aaaactgctg gtgctgtggc gtagtgatga agatgcgcag caacgtctgg atatggtgaa
     9421 agagttcaac gaactgtgcc actcacacgg tctggtaagc atcattgagc cagtcgtccg
     9481 cccaccgcgt cgtggcgata aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga
     9541 gctgggcgac agtggcgctg acctctacaa agttgaaatg ccgctttatg gcaaaggtcc
     9601 gcaacaagag cttctctgtg cttcacaacg tctgaatgac catatcaata tgccatgggt
     9661 gatcctctct tccggtgtcg acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac
     9721 ggcgggcgca tcgggattcc tggcaggccg tgcagtctgg gcatcggtcg tcggtttacc
     9781 agacaacgag ctgatgctgc gtgacgtttg cgcaccgaaa ttacaacaac ttggtgatat
     9841 cgtcgacgaa atgatggcta aacgccgcta agatgaggat aaaaggaatg aaatggttta
     9901 acaccctaag ccacaaccgt tggctggaac aggaaaccga ccgcatcttt gattttggta
     9961 aaaattcagt agtgccgact ggttttggct ggttaggcaa taaagggcaa atcaaagaag
    10021 agatgggcac ccatctgtgg atcaccgctc gtatgttaca cgtttattcc gttgctgcgg
    10081 cgatgggtcg acctggcgct tactcgttgg ttgatcacgg tatcaaagcc atgaacggcg
    10141 cactgcgtga taaaaaatat ggcggctggt atgcctgcgt aaatgacgag ggcgtggtgg
    10201 atgcctccaa acagggctat caacacttct ttgctctgct gggtgccgcc agcgccgtca
    10261 caacgggtca cccggaagcg cgcaagctgc tcgattacac cattgaaatt atcgagaaat
    10321 atttctggag cgaagaagag cagatgtgcc tggaatcctg ggacgaagcc ttcagcaaaa
    10381 ccgaagagta ccgcggcggc aatgccaata tgcacgcggt ggaagctttc ttgattgttt
    10441 atgacgtcac tcacgacaaa aaatggctgg atcgcgcgat tcgcgtggct tccgtgatta
    10501 tccacgacgt cgccagaaat aatcattatc gcgttaacga acacttcgat acccagtgga
    10561 atccgctgcc ggattacaac aaagataacc cggcgcatcg cttccgcgcg ttcggaggta
    10621 caccaggcca ctggatcgaa tggggccgtt taatgctgca catccatgct gccctggaag
    10681 cccgttgcga acaaccacca gcatggctgc tagaagatgc caaaggtctg tttaacgcca
    10741 ccgtgcgcga tgcctgggca cccgatggcg cggacgggat tgtttatacc gttgactggg
    10801 aaggaaaacc ggtggtccgc gaacgtgtac gttggcctat cgtcgaagca atgggtacgg
    10861 cctacgcgct ctacaccgtc actggcgatc gccagtatga aacctggtat caaacatggt
    10921 gggagtactg cattaagtac ctgatggact atgaaaatgg ttcctggtgg caggagctgg
    10981 atgcggacaa taaggtcacc accaaagtct gggacggcaa acaggatatt tatcacctgc
    11041 tgcattgcct ggtgatcccg cgtatcccgt tagcgcccgg catggctcca gcggttgcag
    11101 cgggtctgct ggatattaat gcgaaataac tgatctgaaa atagggtgcg ggttttcccc
    11161 tcaccctaac cctctcccca aaggggcgag gggaccgtcc ggtgcagttt ttgggtttga
    11221 catcagcctc acgggaggct gtatgtcgtt aattaaggtg ccttgtatgc aaatcaccaa
    11281 tatgcattgc agtggacaga ccgtttcact tgccgcgggg gattatcacg cgaccattgt
    11341 gaccgtcggt gctggtctgg ctgaattaac ctttcagggt tgtcatttag tcatcccgca
    11401 caaacccgaa gagatgccgc tggcgcatct gggtaaagtg cttattccgt ggccgaaccg
    11461 gatcgccaac ggctgttacc gctatcaggg gcaggaatat cagttaccca ttaatgaaca
    11521 tagttcaaaa gccgctattc acgggctgct tgcctggcga gactggcaaa ttagtgaatt
    11581 aaccgccacc agcgttaccc tgacagcttt tctaccgccc agttacggct atccgtttat
    11641 gctggcttca caagttgttt attcgctcaa cgcacacacg ggtctttccg ttgagatcgc
    11701 aagccagaat atcggcactg tcgcagcgcc ttatggcgtg ggtattcata cttatttaac
    11761 ctgcaactta acatctgttg atgaatatct gttccagctt ccggcaaacc aggtttatgc
    11821 cgttgatgaa cacgtaaatc cgacaacgct tcaccacgtt gatgagctgg atttaaattt
    11881 cacgcaggcg aaaacaatag ccgcgacaaa aatcgaccac acctttaaaa ccgctaatga
    11941 tctgtgggaa ataaccatta cccatccaca acaggcgctt tcggttagcc tttgttgcga
    12001 tcaaccctgg gtacaaattt atagcggtga aaaattacag cgtcagggac tggcagttga
    12061 acctatgagt tgcccgccta acgcctttaa ttcaggtatc gacttattat tactggaacc
    12121 aggaaaaccg caccgcctgt tttttaacat ttacggacag agaaaataac ttgtattgat
    12181 tagcagtcaa aatcattgaa gaacatttca ctcgctaaag gcaacattat gtatcttcaa
    12241 tttattaagt taaaaacagc tcagcttctg taaagttttt atatatcagt tttatgagaa
    12301 tcattttaca taagcctgta ataacaggaa ggcaacaatc tcgttctcaa ctctatggag
    12361 attagttatg gatacgccac gtccacagtt attagatttt caatttcatc agaataacga
    12421 cagttttacc ctacattttc aacaacgtct tattttaacc catagcaaag ataatccttg
    12481 tttatggatt ggctcaggta tagcggatat cgatatgttc cgcggtaatt tcagcattaa
    12541 agataaacta caggagaaaa ttgcgcttac cgacgccatc gtcagccagt caccggatgg
    12601 ttggttaatt catttcagcc gtggttctga cattagcgcc acgctgaata tctctgccga
    12661 cgatcagggc cgtttattgc tggaactaca aaacgacaac cttaaccaca accgtatctg
    12721 gctgcgcctt gccgctcaac cagaggacca tatctacggc tgcggcgaac agttttccta
    12781 cttcgatctg cgtggcaaac cgttcccgct atggaccagt gaacaaggcg ttggtcgcaa
    12841 caaacaaacc tatgtcacct ggcaggccga ctgcaaagaa aatgcgggcg gcgactatta
    12901 ctggactttc ttcccacagc ctacgtttgt cagcacgcag aagtattact gccatgttga
    12961 taacagttgc tatatgaact tcgactttag tgccccggaa taccatgaac tggcgctgtg
    13021 ggaagacaaa gcaacgctgc gttttgaatg tgctgacaca tacatttccc tgctggaaaa
    13081 attaaccgcc ctgctgggac gccagccaga actgcccgac tggatttatg acggagtaac
    13141 gctcggcatt cagggcggga cggaagtgtg ccagaagaaa ctggacacca tgcgtaacgc
    13201 gggcgtgaag gtcaacggca tctgggcgca ggactggtcc ggtattcgta tgacctcttt
    13261 tggcaaacgc gtgatgtgga actggaagtg gaacagcgaa aactacccgc aactggattc
    13321 acgcattaag cagtggaatc aggagggcgt gcagttcctg gcctatatca acccgtatgt
    13381 tgccagcgat aatgatctct gcgaagaagc ggcacaacac ggctatctgg caaaagatgc
    13441 ctctggcggt gactatctgg tggagtttgg cgagttttac ggcggcgttg tcgatctcac
    13501 taatccagaa gcctacgcct ggttcaagga agtgatcaaa aagaacatga ttgaactcgg
    13561 ctgcggcggc tggatggctg acttcggcga gtatctgccc accgacactt acctgcataa
    13621 cggcatcagc gccgaaatta tgcataacgc ctggcccgcg ctgtgggcga aatgtaacta
    13681 cgaagccctt gaagaaacgg gcaagctcgg cgagatcctt ttctttatgc gcgccggttc
    13741 taccggtagc cagaaatact ccaccatgat gtgggcgggc gaccagaacg tcgactggag
    13801 tctcgacgat ggcctggcgt cggttgtccc ggcggcgctg tcgctggcaa tgaccggaca
    13861 tggcctgcac cacagcgaca ttggcggtta caccaccctg tttgagatga agcgcagcaa
    13921 agagctgctg ctgcgctggt gcgatttcag cgccttcacg ccgatgatgc gcacccacga
    13981 aggtaaccgt cctggcgaca actggcagtt tgacggcgac gcagaaacta tcgcccattt
    14041 tgcccgtatg acctccgtct tcaccaccct gaaaccttac ctgaaagagg ctgtcgcgct
    14101 gaatgcgaag tccggcctgc cggttatgcg cccgctgttc ctgcattacg aagacgatgc
    14161 gcacacttac accctgaaat atcagtacct gttaggtcgc gacattctgg tcgctccggt
    14221 gcatgaagaa ggccgtagcg actggacgct ctatctgccg gaggataact gggtccacgc
    14281 ctggacgggt gaagcgttcc ggggcgggga agttaccgtt aatgcgccca tcggcaagcc
    14341 gccggtcttt tatcgcgccg atagcgaatg ggcggcactg ttcgcgtcgt taaaaagcat
    14401 ctaatcacat ccgcccgtgg ggaaactcat gggcaaaggg agaacatcga tgagtcacat
    14461 cacaacggaa gatccagcaa ctttacgcct gccctttaaa gagaaactct cttacggtat
    14521 tggcgacctg gcctctaaca tcctgctgga tatcggtacg ctttatcttt tgaagtttta
    14581 taccgacgtt ctggggctgc caggcaccta tggcggcatt atctttttga tttcaaaatt
    14641 ctttactgcg tttaccgata tgggtaccgg cattatgttg gattcccgac gcaagatcgg
    14701 tccaaaaggt aagttccgtc cttttattct gtatgcgtca ttcccggtca ccttactggc
    14761 gatcgccaac tttgtcggca caccgtttga tgtcaccggt aaaacggtga tggccactat
    14821 tctgtttatg ctttacggac tgtttttcag catgatgaac tgctcctacg gcgcaatggt
    14881 tcccgctatc accaaaaacc ccaacgaacg cgcctcactg gcggcatggc gtcagggcgg
    14941 cgctacgctg ggcctgctgc tgtgcacggt gggattcgtg ccagtgatga atcttatcga
    15001 gggtaatcag caacttggct atatcttcgc cgccacgctg ttttcactgt tcggcctgct
    15061 gtttatgtgg atctgctact caggcgtgaa agagcgttat gtcgaaaccc aaccaaccaa
    15121 tccggcgcaa aagcctggcc tgttgcaatc tttccgcgca attgccggta accgcccact
    15181 gttcattctg tgtattgcca acctctgcac cttaggggcg tttaacgtca agctcgccat
    15241 tcaggtctat tacacccagt acgtacttaa cgatcccatc ctgttgtcgt atatgggatt
    15301 tttcagcatg ggctgtattt tcatcggcgt gttcctgatg cctggcgcag tcagacgttt
    15361 tggtaagaag aaggtctata tcggcggcct gctgatttgg gtgctgggcg atctgctcaa
    15421 ctatttcttc ggcggcggct cggtcagctt tgtggcgttc tcctgcctgg cgttcttcgg
    15481 ctcagcgttt gttaacagcc tgaactgggc gctggtttcc gacaccgtcg agtacggcga
    15541 gtggcgcacc ggcgtgcgtt cggaaggcac ggtctacacc ggcttcacct tctttcgcaa
    15601 agtgtctcag gcgctggctg gtttcttccc cggctggatg ctgacgcaaa ttggctatgt
    15661 gccgaacgtc gcacaggctg accacaccat tgaagggtta cgccagttga tcttcatcta
    15721 cccaagcgca ctggcggtag tcaccattgt ggcgatgggt tgcttctaca gcctgaacga
    15781 gaagatgtat gtccgcattg tggaagagat agaagcccgt aaacgcacgg cgtaataata
    15841 ataaacgacg ccctgcgggg cgttataagg agtgattatg tctgaccata atccactgac
    15901 attaaaactg aatctgcggg aaaaaatcgc ctatggtatg ggcgacgtcg gttcgaattt
    15961 aatgctctgc atcggtactc tgtatctcct caaattttat accgatgaat tagggatgcc
    16021 tgcttactat ggcgggatta tctttctggt ggcgaagttt tttaccgcgt ttaccgatat
    16081 gctcaccgga tttttactcg actcgcggaa aaatattggg cctaaaggca aatttcgtcc
    16141 ctttatttta tatgcagcag ttccggcggc gttaattgca acgctccagt ttatcgccac
    16201 caccttttgt ttgccggtta aaacgacgat tgccaccgcg ctgtttatga tgtttggact
    16261 ttcatacagt ctgatgaact gctcgtatgg tgcgatgatc ccggcaatta ccaaaaaccc
    16321 gaatgagcgc gcgcagctcg cggcttaccg ccagggtggt gcaaccatag ggctattgat
    16381 ttgtaccgtg gcgtttattc cgttgcagtc gcttttttct gactcaaccg tcggttatgc
    16441 ctgtgcggca cttatgttct ccattggcgg ctttattttt atgatgctgt gctacagagg
    16501 cgtcaaagag cattatgtgg acacagcgcc aaccggacat aaagccagta ttctcaaatc
    16561 tttttgcgcg atatttcgta atccaccatt gctggtttta tgcattgcta acctgtgtac
    16621 tctggcggca tttaatatca aactggcgat tcaggtctat tacacccagt atgtgctgaa
    16681 tgatattaat ttattgtcgt ggatgggatt cttcagtatg ggatgcatcc tcgtcggcgt
    16741 attactggtg ccagtaactg taaaatgttt tggtaaaaaa caggtttatt tagctggcat
    16801 ggtgctatgg gcggtgggtg atatactgaa ttatttctgg ggaagtaact ctttcacttt
    16861 cgtcatgttc tcttgtgtcg ccttttttgg cacggccttt gtaaacagcc tgaactgggc
    16921 actggtacca gataccgtag attacggtga atggaaaacc ggtattcgtg ccgaaggttc
    16981 tgtttatacc ggttatacct tctttcgtaa aatttctgcc gcacttgctg gcttcttgcc
    17041 aggcattatg ctgacgcaaa ttggttatgt tcccaacatc gcgcaaagcg atgcgacatt
    17101 acagggtttg cgtcagctca tttttatctg gccttgtgca ctggcaatta ttgctgcatt
    17161 gacgatggga ttcttttaca cactcaatga aaaacggttt gcattgatta ttgaggaaat
    17221 caaccaacgc aaaaataaag agatggcgac agaagaaaaa acggcttccg taacgttata
    17281 aatgtcaata gccgctattt ccatcctggt gggtggcggc ctccctacgt ttaaaaaatg
    17341 gacttattat gaaaaagatt aatgcaataa ttttattatc ttctctgacc tctgcctcgg
    17401 tatttgctgg cgcatacgtc gaaaacaggg aggcatataa tcttgcctcc gatcagggcg
    17461 aggttatgct acgtgtgggt tataacttcg atatgggcgc gggtattatg ttaaccaata
    17521 cctacacctt tcagcgagaa gatgaactaa aacatggcta taatgaaatt gaaggttggt
    17581 atcctttatt caaaccaacc gataaattaa ccatccagcc cggtggctta attaatgata
    17641 agagtatcgg ttcgggtggt gcagtgtatc tggacgtcaa ctataaattt gtaccatggt
    17701 ttaatctgac agtacgaaat cgctataacc ataataacta tagttcaaca gatttgagcg
    17761 gggaactgga taataatgac acctatgaaa ttggcaccta ctggaatttt aaaatcaccg
    17821 ataaattttc ctatacattt gagccacatt atttcatacg cgtaaatgac tttaatagca
    17881 gcaatggaaa agatcatcac tgggaaatca ctaatacctt ccgttatcgc attaatgaac
    17941 actggctgcc ttatttcgaa ttacgttggt tagatcgtaa tgtcgaaccg taccaccgcg
    18001 agcaaaacca gattcgtatc gggacgaagt atttcttctg atgtgaaact gccggatgcg
    18061 gcgtaaacgc cttatccggc ctacggttga tacggttttc gttggccgga ttaagcacat
    18121 ccggccatta atcaagcttc agccgccagc atctctttct tacgttggct aattcgctta
    18181 accagcatta cggaaaccac cgcaccacag aatgcgaagc aggccataat gccaaacacg
    18241 attttgtagc caataatccc cggatttaaa tccagaatgt agccatacag actgaagcag
    18301 aacatcgccg gagcgtaacc aataaagcta cccaacgcca tcgccgcgcc tgttttattt
    18361 tcagcaattt ttgcttcgcc gataggtgca aaaaatacgg ctcgctgtgt aaagactatc
    18421 gcgccaaagc ccagcgtaca tgccatccct aaatagaccg gcatactttc gtgcggcagc
    18481 ataatcaaca gtacgagcgc agcggtactg atgataaagg tgtagcatag atatttactc
    18541 ggcgatttca ggatcttatc tgaaatcatg ccaccaatcg gtccaccaat cattttcaga
    18601 cagtattggt taatgatgcc gtaagccccc accagcgcaa cgggcaatag atagatgttt
    18661 ttcaggaatg gaatgaagaa tgtcaggcca cagtaaaccg cgtaaacgaa gaagacgtta
    18721 aaagcgataa gccagatggt tttgtctctc agcaccgagg tcatactggt gttttgcgat
    18781 gctccgtctt cttttttcac ctcaacggac ggtgcctctt ctttgtcatt caggacaaag
    18841 aaaataataa tgcctacggc aatcacaata agggaataga accagatgcc tgctttaaaa
    18901 cctaataagc cactgccaaa ccaggtaaat actgccaacg cagaaaatgc caccacggta
    18961 tcgacaatgc cacgccctgt ttcgaagaag ccaaacaacc gaccttgttg ttcgctattg
    19021 cccaatcgac ttaccgactt gagcaagacc ggccagttca tcatgtcgca agtaacgcca
    19081 aatagcgccc agacaaagag gatgccccaa taccccggca ttgtcgtcaa ataaacaccc
    19141 agtaatcctg tcgcaatgag tgagaatgac atggtgtatc tgcgcggtag tttatcggca
    19201 aaataaatag acagaaaaaa gcccactgtg gtgacaaatg agtttaccga catagcatta
    19261 ccaatttgac cattggtcaa atggaaatat tcctgcatcg ggatataaaa cgcatctttc
    19321 agcgacggta atttataaat tgtaccgcca cacaatgtta atagactaaa taacgcccat
    19381 ttctttttcg tgagcataac atcccctaag cgatttttaa aatcactgtt ttaaaggcaa
    19441 taagataccg accattcata tggaatggtt ttattaactg cgtatgagtt aaaacttatt
    19501 agtggttcag aagatggttt gcctcttgtt tgatttgttg tagtacgtta cgcacatgat
    19561 ttatctgatt aattgcatct tctttttctt tacgtaaccg cgcatccagt aaatgatttt
    19621 ctggcaatgg tgtttcactc atctggcgat aagggatcca cggattatca tcttcgtctt
    19681 caaagcggaa cgccggcgca tagtaatcaa cctcttcttc caggccatat gccgtcacct
    19741 gcggctcatc atcacccagg catataaggt gcgttaataa cgctttgaac ggcatatcac
    19801 cctgacctga aatacaggct ttatgaccca ggccacccgg ttctttaacg atcaaggcat
    19861 ctttgatatg gacctgggta atatgcggcg ccatcgtttt taaagcgtcg atgggatgct
    19921 catttgcatt gatcatgttc gcaaaatcaa acagtaagga aagagattcc atctcacttt
    19981 ctttgaccag cgacaccagc tcatggcttt ttaaatcttc atgctgttcg atcgtaaaag
    20041 tcaggccgct gtcctgatac gtttcccgta cataggcaat gtcgttagcg atgatcgata
    20101 atacgtcgcg caaattacct tcataacgtg gataaaaacg tacggacgat gccccagttt
    20161 tcaacgcaat ggcgacggct tcgtcaatag atgccttatc tgaagcgctg gtttcaatat
    20221 gaatatcaag gttcagacgg cgggctttat ccccaaaggc agagagttct ttatcgtcca
    20281 tattaccaag agaaaaacgc tcgccatcca gaacatggat tttgacgcca cgtagattat
    20341 tttcacttgc gatatcaagt aaatcagcag gcaacactct ttccagccgc atatttaaat
    20401 gaaaggcata agcatgtaaa taaagaggaa gagtgtcgac acgttgtaga atctttcttg
    20461 cgttatttat tgtcaccata aaacacctac agattaatga tgaggtctac ggcctcaaat
    20521 cgccaatatt caatatctat ctggacaata ttgccttgct ggtcggcgcg atgcttttca
    20581 attaagatcg ctggcatacc cgcagaaccg ccaagatact tgctgatatc gcccggcatt
    20641 ctgacgggtt tgaaaatcaa tttttttact accgtctctt tttggtagca cttttcccag
    20701 actgcagaaa atgagtggtt ttcaagttgt tcaataaaac ccggagcaac ttcaggatta
    20761 atatatgttt cgtgatagaa aactttatgt ccttccagcg ccccccagcc ggtgatgcga
    20821 tagagttcag ttgatggtgt cactgcaatc agtggcgcga gcgtcttggg aatatcgctg
    20881 gtacggtttt tctcggtaaa cccccaggaa ggctctcgtc cttgctcaat tgcggcccgc
    20941 tgaaagctcg ccgacaactc cggactgtaa ttaaatcgcg gctgggtcac gaaccacccc
    21001 ttacgatcct tacggaagat tttggattct gcctcgaggt tcaacaacgc ctggcgcagc
    21061 gtcatacgtt taatgcccag cagttctccc agctctcttt ctgaagggag ttttccaccc
    21121 ggcgtggtga tgccatcttt caaccaccga tccagtttct cttttgcatt ttctacggtg
    21181 gattgattct ccgccattgt ttaatccttt tggtttaaac cagatgaagc atcttacgct
    21241 tacgccaaaa aagaaagcaa ttaacggcgt atgactttgc agataattgt gacaaagctc
    21301 tctattgacg taatcccaga cttataaggg agatactcgc attggtgtgc gctcacgaca
    21361 ggtaaaaaaa aacctgccag cgatggcagg ttttttataa ctaaaaaacg aaattaatcg
    21421 tctttcggtg cgcggttggc gcggcgacga tcgttttccg tcaggtgacg tttacgaata
    21481 cggatagagg tcggagtcac ttctaccagt tcgtcatcat cgatgaactc cagagcttgt
    21541 tccagagtca tgcggatagg cggaaccaga acaacggctt cgtcagtacc ggaagcacgc
    21601 atgttggtca gtttcttacc ggtcaggcag tttaccgtca ggtcgttaga gcggctatga
    21661 ataccgataa tctgaccttc gtaaacttct gcaccgtgac cgaggaacag cttaccgcga
    21721 tcctgcagac cgaacagcgc gaacgcgacc gctttaccct gaccgttaga gatcagtacg
    21781 ccgttctgac gctgacccac ttcacccgga cgtacgtcgt cgtagtggct gaaggtggag
    21841 tacagcagac cagtaccgga agtcatggtc atgaactcag aacggaagcc aatcagacca
    21901 cggcttggga tcacgtagtc gagacgtacg cggcctttac cgtctggatt catgtttttc
    21961 aggtcgcctt tacgttcgcc cagcgcctgc attacagaac cctgatgctg ttcttcaacg
    22021 tccagagtca cgttttcata cggctcttgt ttacgaccgt cgatttcacg gaagataact
    22081 ttcggacggg ataccgccag ttcgaaacct tcacgacgca tgttttcgat cagaacagac
    22141 aggtgcagtt cgccacgacc agaaacgcgg aacgcatcgg cgtcttcggt ttcttctacg
    22201 cgcagcgcaa cgttgtgtac cagttctttg ttcagacgat ccaggatctg acgagacgtt
    22261 acgaacttac cttctttacc gcagaacggc gaggtgttaa cgcagaagaa catagaaacg
    22321 gtcggctcat caacggagag tgccggcagc gcttcaacgt tttgcgtgtc gcaaacggtg
    22381 tcagaaatgt tcagttcgcc aaggcccgtg atcgcaacga tatcgccagc ttccgccaga
    22441 tcggtttcga tacgttccag accgaggtgg cccagcactt taccgacttt cgcgttgcgg
    22501 gttttgcctt cgctatcgat gatagtgacc tgctggttcg gcttcacttt accgcgcttg
    22561 atgcggccaa tgccgataac gccaacatag ctgttgtaat cgagctgaga aatctgcatc
    22621 tggaacggac cgtcaaggtc aacgtccggc gcaggaacgt ggtcaacaat cgcctggtac
    22681 agcggggtca tgtcttccgc catatcttcg tggtccagac ccgcgatacc gttcagcgca
    22741 gaagcgtaaa cgatcgggaa gtccagctgc tcgtcggtcg cgtcgaggtt aacgaacaga
    22801 tcgaatacct gatccacaac ccaatcagga cgcgcgccag ggcggtcaac tttgttgata
    22861 acaacaatcg gcttcaggcc gtaagcaaac gcttttttgg ttacgaagcg cgtttgcggc
    22921 atcgggccgt caaatgcgtc aaccaccagc agcactgagt ctaccatgga cattacacgt
    22981 tcaacttcac caccgaagtc ggcgtgcccc ggggtatcaa cgatgttgat acggtaatca
    23041 ttccatttga tagcggtgtt tttcgcgagg atggtaatcc cccgctcttt ctccaaatcg
    23101 ttggagtcca tcacgcgctc ttgggtttcg gcacgagagt cgaacgtacc ggattgttgg
    23161 agcagcttgt ctaccagggt ggttttacca tggtctacgt gcgcgatgat ggcgatatta
    23221 cgcaattttt cgatcacaac tttgcctcag gcattagaaa tagcgcgtta ttgtacacgg
    23281 attaatcgca ctacaaaaca ggatcacaaa catcctccgc aaacaagtat tgcagagtcc
    23341 ctttgtgatc gctttcacgg agcataaaaa gggttatcca aaggtcattg caccaacatg
    23401 gtgcttaatg tttccattga agcactatat tggtgcaaca ttcacatcgt ggtgcagccc
    23461 ttttgcacga tggtgcgcat gataacgcct tttaggggca atttaaaagt tggcacagat
    23521 ttcgctttat cttttttacg gcgacacggc caaaataatt gcagatttcg ttaccacgac
    23581 gaccatgacc aatccaggag agttaaagta tgtccgctga acacgtactg acgatgctga
    23641 acgagcacga agtgaagttt gttgatttgc gcttcaccga tactaaaggt aaagaacagc
    23701 acgtcactat ccctgctcat caggtgaatg ctgaattctt cgaagaaggc aaaatgtttg
    23761 acggctcctc gattggcggc tggaaaggca ttaacgagtc cgacatggtg ctgatgccag
    23821 acgcatccac cgcagtgatt gacccgttct tcgccgactc caccctgatt atccgttgcg
    23881 acatccttga acctggcacc ctgcaaggct atgaccgtga cccgcgctcc attgcgaagc
    23941 gcgccgaaga ttacctgcgt tccactggca ttgccgacac cgtactgttc gggccagaac
    24001 ctgaattctt cctgttcgat gacatccgtt tcggatcatc tatctccggt tcccacgttg
    24061 ctatcgacga tatcgaaggc gcatggaact cctccaccca atacgaaggt ggtaacaaag
    24121 gtcaccgtcc ggcagtgaaa ggcggttact tcccggttcc accggtagac tcggctcagg
    24181 atattcgttc tgaaatgtgt ctggtgatgg aacagatggg tctggtggtt gaagcccatc
    24241 accacgaagt agcgactgct ggtcagaacg aagtggctac ccgcttcaat accatgacca
    24301 aaaaagctga cgaaattcag atctacaaat atgttgtgca caacgtagcg caccgcttcg
    24361 gtaaaaccgc gacctttatg ccaaaaccga tgttcggtga taacggctcc ggtatgcact
    24421 gccacatgtc tctgtctaaa aacggcgtta acctgttcgc aggcgacaaa tacgcaggtc
    24481 tgtctgagca ggcgctgtac tacattggcg gcgtaatcaa acacgctaaa gcgattaacg
    24541 ccctggcaaa cccgaccacc aactcttata agcgtctggt cccgggctat gaagcaccgg
    24601 taatgctggc ttactctgcg cgtaaccgtt ctgcgtctat ccgtattccg gtggtttctt
    24661 ctccgaaagc acgtcgtatc gaagtacgtt tcccggatcc ggcagctaac ccgtacctgt
    24721 gctttgctgc cctgctgatg gccggtcttg atggtatcaa gaacaagatc catccgggcg
    24781 aagccatgga caaaaacctg tatgacctgc cgccagaaga agcgaaagag atcccacagg
    24841 ttgcaggctc tctggaagaa gcactgaacg aactggatct ggaccgcgag ttcctgaaag
    24901 ccggtggcgt gttcactgac gaagcaattg atgcgtacat cgctctgcgt cgcgaagaag
    24961 atgaccgcgt gcgtatgact ccgcatccgg tagagtttga gctgtactac agcgtctaat
    25021 agttgaagtt gtactacccg gcgcaacaac gccggaggtt caaaccaggc ccatctggcg
    25081 taattgttgc agccagtttg aacacggaca gcgcgcagaa cccggagcgt acactggtac
    25141 gtgaggagtt cgagcactgc ccaggttcaa aatggcaaat aaaatagcct gatgggactg
    25201 gtttttagtt gccgtggaaa tttcagccca tctctgcatg ggcttttttc tccgtcaatt
    25261 ctctgatgct tcgcgctttt tatccgtaaa aagctataat gcactaaaat ggtgcaacct
    25321 gttcaggaga ctgctttatg gcaacaggca cgcagcccga tgctgggcag atcctcaact
    25381 cgctgattaa cagtattttg ttaatcgatg acaacctggc gatccattac gccaaccctg
    25441 ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt tggtacaccg ttaccggaac
    25501 tgttgagcta cttctcatta aatatcgagc tgatgcaaga aagtctggag gcggggcaag
    25561 gttttaccga taacgaagtg acgctggtca tcgacgggcg ctcgcatatc ctttctgtga
    25621 cggcccagcg tatgccggac ggcatgatcc tgctggagat ggctccgatg gataaccagc
    25681 gccgcttaag tcaggaacag ctacagcacg cccagcaggt tgctgcccgt gatttagtgc
    25741 gcggcctggc acatgagatt aaaaatccgc ttggcggttt acgtggcgcg gcgcagctgc
    25801 tcagcaaagc gttacctgac ccatcactac tcgaatatac caaagtgatt atcgaacagg
    25861 cggaccggct gcgaaatctg gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgcg
    25921 ttaccgaaag tattcacaaa gtggctgaac gcgtggtaac gctggtgtcg atggaactgc
    25981 cggacaacgt gcggttgatt cgtgattacg atcccagcct gccggaactg gcgcacgacc
    26041 cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc
    26101 cggaaggcgg tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg
    26161 agcgctaccg gctggcggcg cggattgatg tggaagataa cgggccgggc attccgcctc
    26221 atttgcagga tacgctgttt tacccgatgg tcagcggccg cgaaggtggc accgggcttg
    26281 gcttatccat cgctcgtaat ttgattgatc agcattcagg caaaattgaa tttaccagtt
    26341 ggccagggca taccgagttc tcggtttacc tgcctatcag gaaataaagg tgacgtttat
    26401 gcaacgaggg atagtctggg tagtcgatga cgatagttcc atccgttggg tgcttgaacg
    26461 tgcgctcgct ggggcaggtt taacctgtac gacgtttgag aacggcgcag aagtgctgga
    26521 ggcgctggcg agcaaaacgc cggatgtgct gctttcagat atccgtatgc cgggaatgga
    26581 cgggctggcg ctgctcaagc agattaaaca gcgccatcca atgcttccgg tcatcattat
    26641 gaccgcacat tccgatctgg atgctgccgt cagcgcctat caacaagggg cgtttgatta
    26701 tctgcccaaa ccgtttgata tcgacgaagc agtggcgctg gttgagcgcg ctatcagtca
    26761 ttaccaggaa cagcagcagc cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat
    26821 cggcgaagcg ccagccatgc aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc
    26881 tattagcgtg ctgattaacg gcgaatccgg caccggtaaa gaactggtcg ctcatgccct
    26941 gcatcgccac agtccgcgcg ccaaagcgcc gtttatcgcg ctgaatatgg cagctatccc
    27001 aaaagatttg atcgaatcag aactgtttgg ccacgagaaa ggcgcgttta ctggcgcgaa
    27061 taccattcgt caggggcgtt ttgaacaggc cgatggcggt acattattcc tcgacgaaat
    27121 tggtgatatg ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt
    27181 ttaccgcgtt ggcggctatg cgccggtgaa agtggatgtg cggattatcg ctgccactca
    27241 ccagaatctc gaacagcgag tgcaggaagg taagttccgt gaggatctgt tccaccgcct
    27301 gaacgttatc cgcgttcatc tgccgccgct gcgcgaacgt cgggaagata ttccccgtct
    27361 ggcgcgccat tttttacagg ttgccgcgcg cgaactgggc gtagaagcga agttactgca
    27421 tccggaaacc gaagctgctc tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga
    27481 aaacacctgc cgctggctaa cggtgatggc cgccgggcag gaagtgttga ttcaggattt
    27541 gcccggcgaa ctgtttgaat caacggttgc ggagagtact tcgcaaatgc aaccggacag
    27601 ctgggcgacg cttcttgcgc agtgggcaga cagagcgctg cgttccggtc atcaaaatct
    27661 gctttccgaa gcgcagccag agctggagcg gacgttactg acgaccgcgt tgcgacatac
    27721 gcaggggcat aaacaggaag cggcgcggct actcggctgg ggccgcaaca ccctgacgcg
    27781 taagttaaaa gagctgggga tggagtgatt cacagcttgt gtgtaaagat tgattattga
    27841 gcgcaaattg ctggtatttt acgctttact gttccgataa gttcagtatg atcttgcccg
    27901 gaaaacgggg agagtcatta tgctggaatc aataattaat ctggtatcga gtggcgcagt
    27961 tgacagccac acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcaa tgattgggct
    28021 gtttagttga gtttcagatt gctgatgaag tgctctttgt tcatgccgga tgcggcgtga
    28081 acgccttatc cggcctacaa aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt
    28141 agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc tactttgtaa
    28201 acgaagcgcc attcactatt taaatcaccc gtgagaactg ctgcatccgc gctttctggc
    28261 gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct ttcgccgtca
    28321 cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg gcgagcagct
    28381 tgagatcttc cgcaaagtaa tcagcgaagt gcaaatccca cagtttctca ataggggcgt
    28441 aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata cagtcatcac
    28501 gcgttagcgc aataccacgc cacagcgcat tgccttgttc atccacttgc tgatagtact
    28561 gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc
    28621 ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta cgatgcagca
    28681 cgccttcacg ctgggccacc gccagctcgt catccggacg ggcaaagtga tccataccga
    28741 taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata tcgagttttt
    28801 gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa atggtcggca
    28861 gatgcgcgta gttaaagaca ctcagacgat cggggttcag ctccgccaca cgtttcaggg
    28921 taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg
    28981 aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct tcatcctgct
    29041 cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg ctcaggcgat
    29101 taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc ggatcgactt
    29161 cgatcgaaat ctccgcatcg gcattgaact ggaagttttc gcgcagcagc ttcatcaggc
    29221 ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc aattggctga
    29281 cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc gcgtccagat
    29341 actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa
    29401 gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga tagcgcgcca
    29461 cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg
    29521 aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc cagtcgattt
    29581 gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc gtaccggcgg
    29641 atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg acaaccgctg
    29701 tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca gcagaacggg
    29761 aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt
    29821 ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct gcatcaactc
    29881 atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca gggtttcgcc
    29941 tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta attccaactc
    30001 cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg tgactttttc
    30061 agtcacgccc aatggaatag gggttttact gccaatacgt ggatcttttg gtgcgttctg
    30121 gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg catgaccacg
    30181 acgttttttc tgacgcttgc gatcacgagc ttcctgatcc agctcctcgc gtgtttttcg
    30241 acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca taatggttcg
    30301 tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag
    30361 aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct
    30421 tacaatcctt gcaggccgcc aacacaccct gtttttcctt tagccggaac aatccgtgtt
    30481 gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt
    30541 cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct
    30601 atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca gtatttgcac
    30661 attttcagag ttttatataa aagcaattca ctgatttata tagcttattt gacatgcatc
    30721 gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca aagcgtatgg
    30781 cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca ggtataatcg
    30841 ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat caacagacgc
    30901 attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga attgaagtgg
    30961 cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg actaaccaga
    31021 aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac ctgtttgaag
    31081 tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag
    31141 agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt cagagcctgc
    31201 aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat cagcagatga
    31261 ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa gcggacaaac
    31321 tggcaagcgg cgcacgtaaa gcgcaattga atatggtgcg tgaagctgta ctggcgttta
    31381 acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgtg gacaagctgc
    31441 ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg caggacggcg
    31501 aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg taaattcaat
    31561 agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac ggatcttttc
    31621 tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa tattcagcca
    31681 aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct gtaccctacg
    31741 cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt ttttttcagt
    31801 tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt gcttacttac
    31861 gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc gccactcccc
    31921 acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg atgaatctgc
    31981 ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc gtgtacctgc
    32041 atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc aatcatcgcc
    32101 cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc acgttcagcc
    32161 gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg gcgtccgtcc
    32221 agcgtttcaa cgtagccctg ctctttcgcc tgagcacggg tgcgttccat atactccagc
    32281 acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg
    32341 ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa gttgatcgct
    32401 ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaactaaa cacttctgcc
    32461 gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc tttgtcacgc
    32521 gaaagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac aatcacataa
    32581 tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg caccggaatg
    32641 ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc ctggtgataa
    32701 gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc ggtgtaggtc
    32761 gatttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg atagtccagc
    32821 gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc
    32881 ggtttaatac cctgtttttc aaagagaatg gtttgcaact gcttggtgga agaaaggtta
    32941 aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg caaggtgagc
    33001 tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg ttcaatgcgt
    33061 gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg ccctttgtgt
    33121 ttttgcagat ctggccacat tttcagatgc aactgcaagg tgacatcagc atcttcggcg
    33181 gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt tttgccttta
    33241 cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc gaggctgtcc
    33301 atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt atcaaacgca
    33361 atcccacgca gctcaatgcc gtaattcgcc agaataccgc gatcgtattt caggttttgc
    33421 ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag tgcacgctcg
    33481 cgagagattt gatcgggcgc gtcaagataa tcatgagcaa ccggaatata tgccgctacg
    33541 cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc aaggctgtcg
    33601 gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca cgctttcagt
    33661 gtttcttcat caaggatggt gacgtagttg tcataagaaa tcaccgttgc cgtcacttct
    33721 ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctggttttgc ccctttggcc
    33781 tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata ctttttgaac
    33841 agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca ggtcagctcc
    33901 agttcaacgt cggttttaat cgtcgccagc tggtatgaga gataagcaac ttctttgttt
    33961 tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc aattttttct
    34021 ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg cgcggttttt
    34081 tcaccgacgc ccggtacgcc aggaatgtta tcggaggagt cacccatcag cgccaggaaa
    34141 tcgatgatca gttctggcgg cacgccgtac ttattcacca cctcttccgg tccgaggatg
    34201 gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc catatcttta
    34261 tcgccagtgc tgatcagcac cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg
    34321 ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat cgctttaacc
    34381 atcgcgtgca ggggttcgat ttgtgctcgc agatcgttcg gcattggcgg gcgatgtgat
    34441 ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa gaccaccgct
    34501 gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac accatacatc
    34561 gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata tgcgcgataa
    34621 agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac cataatgtcc
    34681 gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa catgagtttt
    34741 tcaccgcttt tcgtgaaaat cttttgtaga tcttctggat cgctcgcaaa tcgtcatgtg
    34801 gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc tttttacagc
    34861 atgatatttg tctttgtttt tcatgcagtt aaaaataaag ccatccctaa taattgagat
    34921 aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag gatcatccgt
    34981 tatttgggtc gttttctgct aaaatctgcg ccttgcgctt ctttagcgca ctgtctatgg
    35041 ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc cttttgctga
    35101 gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc attgccggga
    35161 ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt
    35221 ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac ccacacctgc
    35281 aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg cttatttgta
    35341 accaccgtag ttgggcag
//
LOCUS       DAZFJF010000037        34994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFJF010000037 DAZFJF010000000
VERSION     DAZFJF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.037"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            133..717
                     /gene="yadN"
                     /locus_tag="ACHKAU_002017"
     CDS             133..717
                     /gene="yadN"
                     /locus_tag="ACHKAU_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5531082.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            822..1562
                     /gene="yadV"
                     /locus_tag="ACHKAU_002018"
     CDS             822..1562
                     /gene="yadV"
                     /locus_tag="ACHKAU_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5531083.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1597..4197
                     /gene="htrE"
                     /locus_tag="ACHKAU_002019"
     CDS             1597..4197
                     /gene="htrE"
                     /locus_tag="ACHKAU_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO5531084.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4214..4783
                     /gene="yadM"
                     /locus_tag="ACHKAU_002020"
     CDS             4214..4783
                     /gene="yadM"
                     /locus_tag="ACHKAU_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5531085.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4798..5400
                     /gene="yadL"
                     /locus_tag="ACHKAU_002021"
     CDS             4798..5400
                     /gene="yadL"
                     /locus_tag="ACHKAU_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO5531086.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5427..6023
                     /locus_tag="ACHKAU_002022"
     CDS             5427..6023
                     /locus_tag="ACHKAU_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5531087.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6007..7350
                     /locus_tag="ACHKAU_002023"
                     /pseudo
     CDS             6007..7350
                     /locus_tag="ACHKAU_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            7463..8257
                     /gene="panB"
                     /locus_tag="ACHKAU_002024"
     CDS             7463..8257
                     /gene="panB"
                     /locus_tag="ACHKAU_002024"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO5531088.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8269..9120
                     /gene="panC"
                     /locus_tag="ACHKAU_002025"
     CDS             8269..9120
                     /gene="panC"
                     /locus_tag="ACHKAU_002025"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO5531089.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9194..10096)
                     /gene="rpnC"
                     /locus_tag="ACHKAU_002026"
     CDS             complement(9194..10096)
                     /gene="rpnC"
                     /locus_tag="ACHKAU_002026"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO5531090.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10370..10750
                     /gene="panD"
                     /locus_tag="ACHKAU_002027"
     CDS             10370..10750
                     /gene="panD"
                     /locus_tag="ACHKAU_002027"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO5531091.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10754..11983)
                     /gene="yadE"
                     /locus_tag="ACHKAU_002028"
     CDS             complement(10754..11983)
                     /gene="yadE"
                     /locus_tag="ACHKAU_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5531092.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12047..12487)
                     /gene="yadI"
                     /locus_tag="ACHKAU_002029"
     CDS             complement(12047..12487)
                     /gene="yadI"
                     /locus_tag="ACHKAU_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5531093.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12592..13362)
                     /gene="yadH"
                     /locus_tag="ACHKAU_002030"
     CDS             complement(12592..13362)
                     /gene="yadH"
                     /locus_tag="ACHKAU_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5531094.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13359..14285)
                     /gene="yadG"
                     /locus_tag="ACHKAU_002031"
     CDS             complement(13359..14285)
                     /gene="yadG"
                     /locus_tag="ACHKAU_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5531095.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14394..15056
                     /gene="can"
                     /locus_tag="ACHKAU_002032"
     CDS             14394..15056
                     /gene="can"
                     /locus_tag="ACHKAU_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO5531096.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15097..15633)
                     /gene="hpt"
                     /locus_tag="ACHKAU_002033"
     CDS             complement(15097..15633)
                     /gene="hpt"
                     /locus_tag="ACHKAU_002033"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO5531097.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15839..18229
                     /gene="gcd"
                     /locus_tag="ACHKAU_002034"
     CDS             15839..18229
                     /gene="gcd"
                     /locus_tag="ACHKAU_002034"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO5531098.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVQFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18276..19826)
                     /gene="cueO"
                     /locus_tag="ACHKAU_002035"
     CDS             complement(18276..19826)
                     /gene="cueO"
                     /locus_tag="ACHKAU_002035"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO5531099.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19992..20339
                     /gene="yacC"
                     /locus_tag="ACHKAU_002036"
     CDS             19992..20339
                     /gene="yacC"
                     /locus_tag="ACHKAU_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO5531100.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20445..21311
                     /gene="speE"
                     /locus_tag="ACHKAU_002037"
     CDS             20445..21311
                     /gene="speE"
                     /locus_tag="ACHKAU_002037"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO5531101.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21327..22121
                     /gene="speD"
                     /locus_tag="ACHKAU_002038"
     CDS             21327..22121
                     /gene="speD"
                     /locus_tag="ACHKAU_002038"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO5531102.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22159..22521)
                     /gene="yacL"
                     /locus_tag="ACHKAU_002039"
     CDS             complement(22159..22521)
                     /gene="yacL"
                     /locus_tag="ACHKAU_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO5531103.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22696..25293)
                     /gene="acnB"
                     /locus_tag="ACHKAU_002040"
     CDS             complement(22696..25293)
                     /gene="acnB"
                     /locus_tag="ACHKAU_002040"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO5531104.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25648..27405
                     /gene="yacH"
                     /locus_tag="ACHKAU_002041"
     CDS             25648..27405
                     /gene="yacH"
                     /locus_tag="ACHKAU_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO5531105.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27476..28900)
                     /gene="lpdA"
                     /locus_tag="ACHKAU_002042"
     CDS             complement(27476..28900)
                     /gene="lpdA"
                     /locus_tag="ACHKAU_002042"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO5531106.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29225..31117)
                     /gene="aceF"
                     /locus_tag="ACHKAU_002043"
     CDS             complement(29225..31117)
                     /gene="aceF"
                     /locus_tag="ACHKAU_002043"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO5531107.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31132..33795)
                     /gene="aceE"
                     /locus_tag="ACHKAU_002044"
     CDS             complement(31132..33795)
                     /gene="aceE"
                     /locus_tag="ACHKAU_002044"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO5531108.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33956..34720)
                     /gene="pdhR"
                     /locus_tag="ACHKAU_002045"
     CDS             complement(33956..34720)
                     /gene="pdhR"
                     /locus_tag="ACHKAU_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO5531109.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8799 a         9052 c         8397 g         8746 t
ORIGIN      
        1 acaatggttg taaattttgg taacaaatat cattgctcat taatatctag catctgctta
       61 taacatttgt gtcatgaata gtcattcttc aaaccagtag tctgactaat ggaattttat
      121 tcaaggattt ttatgtctaa aaaattaggt tttgccctgt ctggtcttat gctggcaatg
      181 gttgcaggta ctgcttctgc tgatatggat ggtggtcagt taaatatcag tggtctggtt
      241 gttgataaca cctgtgaaac ccgcgttgac ggcggtaata aagatggtct gatcctgctg
      301 caaactgcaa ctgttggtga aatcgatgct ggcgtgctga atgacaccgt tggcgctaaa
      361 gccaaacctt tcagcatcac cgttgactgc agcaaagcaa atccgaatcc aggtagtaca
      421 gctaaaatga ccttcggttc tgttttcttt ggtaatagca aaggaacgct gaacaacgac
      481 atgtctatta acaacccgtc tgacggcgtg aatatcgctc tgcacaatat tgatggttcc
      541 actatcaaac aggttcaaat taacaaccct ggcgatgtgt atactaaagc cctggatgca
      601 acgacaaaat ctgctgttta tgattttaaa gcgtcttacg ttcgtgccgt cgcagaccaa
      661 acagcaactg ctggttatgt aaaaactaac actgcataca ccattactta tcagtaagta
      721 ttcaccaatt aaattgattg aatgaatata cagggaatag tgttaataat ttctattttt
      781 atattattcc ctgttttaat taactctgtc agggatggtt tatgtttttt aacaccaaac
      841 atacaacagc tttatgcttc gtaacctgta tggcttttag ttcatcgtct attgcggaca
      901 ttgtcatttc gggtactcgc gtaatatata aaagcgatca aaaaagtgtc aacgtacgtc
      961 tggaaaataa agggaataac ccgttgcttg tccagagttg gttagatact ggcgatgaca
     1021 acgctgagcc tggcagtatt acagtccctt ttactgccac gccgccagta tcgcgtattg
     1081 atgccaaacg tgggcaaaca atcaaattaa tgtacacagc cagcacctca ctgcctaaag
     1141 acagagagag cgtgttctgg tttaacgtac tggaagttcc accaaaacca gatgcagaaa
     1201 aggtcgcgaa tcaaagtctg ctgcaactgg catttcgcac acgtataaaa cttttctatc
     1261 gcccggatgg attgaaggga aatccctctg aagccccgtt agccctgaag tggttctggt
     1321 caggttcaga aggtaaggca tcattgcgag tgaccaatcc aaccccttac tacgtctctt
     1381 ttagcagtgg tgatttagaa gctagcggta aacgctatcc gattgatgtg aaaatgattg
     1441 caccatttag tgatgaggtc atgaaagtca ctggccttaa tggcaaagca agttctgcaa
     1501 aagtacattt ttacgccatt aatgattttg gtggcgccat tgaaggtaat gccagtctgt
     1561 aatcaggcaa ggatataatt ccgcaggaag cataacgtga ctataaaacc aactaaaaat
     1621 tatcataatc atctgacccg tatcgctacg ttttgcgcgc tgctgtattg caatagtgct
     1681 ttctgtgctg aacttgttga atatgaccat accttcctga tggggcagaa tgcctcaaat
     1741 attgatctca gccggtatag tgaaggtaac cctgctatac cgggtatgta cgacgtcagt
     1801 gtttatgtca acgatcaacc tattattaac caaagcatta catttattga aattgaagga
     1861 aaaaaaaacg cacaagcctg tatcacactt aagaatttat tgcagtttca tataaattct
     1921 cccgatataa ataacgaaaa agccgttctg cttgccaggg atgaaacact cggcaattgc
     1981 cttaatttga cggaaattat ccctcaggct tctgttcgtt atgacgttaa cgagcaacgt
     2041 ctggatatag acgttcctca ggcatgggta atgaaaaatt accaaaacta tgttgatcca
     2101 tcgttatggg aaaatggcat taatgcggcc atgttgtcat acaacctcaa cggatatcat
     2161 agtgaaactc ctggtcgaag aaatgacagc atttatgctg cttttaacgg tgggatgaat
     2221 ttaggtgcat ggcgactgcg tgcctcaggc aactacaact ggatgaccga ttctggcagt
     2281 aattatgatt ttaagaatcg gtatatacag cgtgatatcg cctcgctgcg ttctcaactc
     2341 attcttggtg agtcttatac aacgggcgaa acctttgatt ctgtcagtat ccgcggcatt
     2401 cgtttataca gcgacagccg catgttgcct ccgacattag cgagttttgc gcctatcatt
     2461 catggcgttg ctaataccaa cgccaaagtc actattacgc aaggtggcta taagatttat
     2521 gaaacgactg tgccgccagg cgctttcgtc attgatgatc tgagtccgtc aggttacggc
     2581 agcgatctta ttgttactgt cgaagaatcc gatggctcaa agcggacatt ctcgcaacct
     2641 ttttcatcgg tcgttcaaat gttacgccct ggcgttggac gttgggatat tagcggcggc
     2701 caggtcttaa aagatgatat tcaggatgag cctaatttat ttcaggccag ctactactac
     2761 ggtctgaata actatctaac gggttatact ggtattcaga ttaccgataa taactatacc
     2821 gctggtttgt taggtcttgg tctgaatact tcagtcggtg cattttcttt cgatgtgact
     2881 cactccaatg ttcgtatacc ggatgataaa acatatcagg gacaaagtta tcgtgtttcc
     2941 tggaacaagt tattcgaaga aacaagtact tcactgaata tcgcggccta tcgctattcg
     3001 acacagaatt accttggtct taatgatgca ctaactctaa ttgatgaagt gaaacatccc
     3061 gaacaagatc ttgaaccgaa atccatgcgt aattactcgc gcatgaaaaa tcaggttacg
     3121 gtcagtatta accaaccgct aaaatttgag aaaaaagatt acggttcatt ttatctttcc
     3181 ggaagttggt ccgattactg ggcttccgga caaaatcgta gcaattactc tattggctac
     3241 agtaacagtg catcctgggg cagctacagt gtcagtgccc agcgttcatg gaatgaagac
     3301 ggcgacactg acgatagcgt ttatctcagt ttcaccattc ctattgaaaa attacttggc
     3361 actgaacaac ggacttcagg tttccagagt attgatactc aaatgagcag tgactttaag
     3421 ggtaataacc aactcaacgt cagtagcagt ggctatagtg ataacgctcg cgtcagttat
     3481 agcgtaaata ctggctatac gatgaataaa gccagcaaag atttgagtta tgttgggggt
     3541 tatgccagct atgaatcacc gtggggaacc ctggcaggtt cagtttctgc aaatagcgat
     3601 aacagtcgtc aagtgtctct cagcaccgac ggtggttttg tattgcatag cggcggcctg
     3661 accttcagta atgacagttt tagcgactcc gatacactgg cggtagttca ggctccagga
     3721 gctcaaggag cgcgaataaa ttatggcaac agtactatcg atcgatgggg ttatggtgtc
     3781 accagcgctc tttctcctta tcatgaaaac cgtatcgcgc tggatatcaa cgatcttgag
     3841 aacgatgttg aattaaaaag taccagtgca gtagctgtac cgcgtcaggg ttcagtcgtc
     3901 tttgctgatt ttgaaaccgt gcaagggcaa tcagccatta tgaacatcac acgaagtgat
     3961 ggtaaaaata ttccatttgc tgcagatatt tatgatgagc aaggcaatgt cattggtaat
     4021 gttggacagg gtggacaagc atttgttcgt ggtattgagc agcagggaaa tatcagcatt
     4081 aaatggctcg aagaaagtaa acccgtaagt tgtcttgcgc attatcaaca aagttcagaa
     4141 gcagaaaaaa tagcgcaatc tattattctg aatggaattc ggtgtcagat tcagtaacta
     4201 caagggacgt caaatgataa aaataacgcc acataaaata gcgatactga tgggaatatt
     4261 attatcacca tcagtatttg caacggatat taatgtagag tttacagcca ctgtcaaagc
     4321 gacaacctgt aacatcacac ttactggtaa taacgtcacg aatgatggca ataataacta
     4381 cacattgaga atccctaaga tgggtctgga taagatcgcg aataaaacga cagaatctca
     4441 ggctgatttt aaactggttg ctagtgggtg cagcagtggc atcagttgga ttgataccac
     4501 tctgagcgga aatgcatcat caagctcacc taagcttatt ataccgcagt ctggtgattc
     4561 atcttcgacg acaagtaata tcggtatggg tttcaaaaaa cggactactg atgatgccac
     4621 tttccttaaa cctaacagtg cggaaaagat acgctggagc acagacgaga tgcagcccga
     4681 taagggtctt gaaatgaccg ttgcgctacg tgaaacagat gcagggcaag gcgtaccggg
     4741 gaattttcgt gcactggcaa cgtttaattt catctatcaa taagaaggat aattaagatg
     4801 acatttaaaa atttacgtta tggattatcc agcagtgtcg ttttggctgc ctcattgttc
     4861 agcgtaccct cttatgcggc aacagacagc attgggctga ccgtgattac taccgtagaa
     4921 ataggtactt gtaccgctac attagtaaat gactctgatc aggacatttc tgttgttgac
     4981 tttggcgatg tatatatttc tgaaatcaat gccaagacca aagttaaaac attcaaactc
     5041 aaattcaaag actgtgcggg tatccccaat aaaaaagcac aaataaaatt aaccaagcga
     5101 gccacatgcg aaggtactgc taatgacggt gcggggttcg caaatggatc cacagccgca
     5161 gataaagcaa gtgccgtcgc cgttgaagtc tggagcaccg aaactccggc aacagggagc
     5221 gcaacacaat ttagttgtgt gacaccagca tctcaagagg tcactatctc caatgcagcc
     5281 aacgcggttg tttattatcc gatgagtgct cgactggtcg tcgaaaaaaa taaaaccgta
     5341 agcaatgtca ctgcgggtaa attttctgca cctgccacat ttacagtaac ctataactaa
     5401 cagactgaca gatccaggaa ctatatatgc atcccactca acgtaagctg atgaagagaa
     5461 taattctgtt tctgtcacta cagtttccca ttacctatcc agccatcgct gggcaggata
     5521 ttgaccttgt tgccaatgta aaaaacagca cttgcaaaag cggaatcagt aaccagggta
     5581 atattgatct tggcgtcgtt ggggtgggat atttttcaga taatgttact cctgaaagct
     5641 atcaaccagg tggaaaagag ttcactgtca cagtatcaga ctgtgcatta cagggaactg
     5701 gcgatgtgct taatcagtta catattgatt ttagagccct tagcggtgtc atggctgcag
     5761 gttcaaggca aatatttgcc aatgaagtcg caaccggggc taaaaatgtc ggtgtcgttc
     5821 tcttttccat tcaggacccg accaatatat tcaatgttat tagttcagca ggaaattctc
     5881 gttccgtgta tccagtaatg acttctgccc tgcataattc ttcatggaaa ttttatgcca
     5941 gaatgcaaaa aatcgatccc gcgctggatg tcatatctgg ccaggtaatg agtcatatat
     6001 tagttgatgt gtactacgaa taatagatca aaacaaataa ctgcgactgt tacagcagta
     6061 gatggtggag tgataatgaa gaaattcttc agacattttc tttttttaat tctgtgcttg
     6121 agttgttata ccgcgagtgc tggcactgat gataacgtag gatatattgt tgggaatagt
     6181 tacggcgttg gtccgtcaga tcaaaaatgg agagaaacgg ggccgaatgg cgatgctact
     6241 gtcatattca gatatgcaac aagtacaaat aatttagttt tctacaaacc gacgcagctt
     6301 ggacctacag gtgtaaaatt acagtggagt cagttagata ccgcttctgg tggtggtttt
     6361 ctttattgca acagaagtga tagcacaagt ggtagcgcaa tgcgtattga aaatgcaatg
     6421 gttgactcag gtaaaatgta tggctcccat aaattattta atacatcagt tcctggtttg
     6481 tattacacat tattaatttc aaacatgtgg tcagcttacg gtaccgtaac taacgttagt
     6541 tcacctggga tatacattgg tgactctgca gaacaatatt tttcgtggta taatccaagc
     6601 gaagacgtgt tatattagag ttgcaataat gcgaatagca cccgtaaata ctgggctgta
     6661 ggtggtattt atcagaccct tacaattgaa ttctatacag atacaaactt tgatccaaca
     6721 gtaactcaac agatcaaatt atccagctca tcaaattatc tgtattcatt taaagcctat
     6781 ggcgcaggcc aaggtataaa tgagcatagt tattttatca aaatcgattt cgatctgctc
     6841 aatgtcaagt tgactaatcc cacttgtttt accgctatgc ttagtggaac ttctgtgact
     6901 ggttctacgg taaaaatggg tgaatatagt gcagaacaaa tcagaaacgg tgccacaccg
     6961 gttccttttg atatttcact tcaaaactgc gttcgtgtga ctaatattga gacaaaatta
     7021 gtttcaacaa aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat
     7081 gacgcagcaa aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta
     7141 atgacattga aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa
     7201 gacgatacaa caggaggagt ttacccggat caagatacag gtataacata ccctctccat
     7261 ttccaggcca cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc
     7321 accagtactt tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg
     7381 tgcagtaatt gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg
     7441 acatcattta tcaggatacg ttatgaaacc aaccaccatt gcttcacttc agaaatgtaa
     7501 gcaggataaa aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt
     7561 tgctgatgaa ggacttaacg tcatgctggt aggcgactcg ctgggcatga cggttcaggg
     7621 tcacgactcc accctgccgg tgaccgttgc tgatatcgcc taccacactg ccgccgtacg
     7681 tcgcggcgcg ccaaactgcc tgctgctggc tgatctgccg tttatggcgt atgccacgcc
     7741 ggaacaagcc tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat
     7801 tgagggcggt gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt
     7861 atgtggtcac ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagttca
     7921 ggggcgcggc gatgaagcgg gcgatcaact gctcagcgat gcattagcct tagaagccgc
     7981 tggggcacag ctgctggtgc tggaatgcgt gccggttgaa ctggcaaaac gtattaccga
     8041 tgcactggcg atcccggtta ttggcattgg cgcaggcaac gtcactgacg ggcagatcct
     8101 cgtgatgcac gacgccttcg gcattaccgg cggtcacatt cctaaattcg ctaaaaattt
     8161 cctcgccgaa acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc
     8221 cggcgtttat ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc
     8281 gaaaccctgc cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg
     8341 gcgctggtgc ctactatggg taatctgcac gatggccata tgaagctggt cgacgaagcc
     8401 aaagcccgcg ccgatgtggt cgtcgtcagt attttcgtta acccaatgca gtttgaccgc
     8461 ccggaagatc tggctcgtta tccacggacc ttgcaagagg actgcgaaaa gctaaacaaa
     8521 cgtaaagtgg atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa
     8581 acccacactt acgttgacgt tcctggcctt tcgaccatgc tggaaggtgc cagccgtccg
     8641 ggacattttc gcggcgtttc gactattgtc agcaagctgt tcaacctggt ccagccggac
     8701 atcgcctgct tcggtgaaaa ggactttcag caactggcgc taatccgcaa aatggttgcc
     8761 gatatgggct tcgatattga aattgtcggc gtcccgatta tacgcgccaa agatggcctt
     8821 gcgctcagtt cccgtaatgg ttatctgacg gcggaacaac gcaaaattgc gcccggtctg
     8881 tacaaagttt taagttcaat tgccgacaaa ttgcaggctg gggaacggga tctcgatgaa
     8941 attattacca ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag
     9001 attcgcgatg ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg
     9061 gtagccgcct ggcttggcga tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa
     9121 tacttaactg gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca
     9181 taacacaggt aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg
     9241 ccatctctgc aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt
     9301 gcctttcctc ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact
     9361 gcgccagcgt catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca
     9421 aatccgcttg ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac
     9481 ttccgctagt gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt
     9541 cgcgctggcg aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga
     9601 tttcgtcatc cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc
     9661 gtgccagctc cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg
     9721 cctcgccctg ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca
     9781 gatgccggtg catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg
     9841 gcttgctttg gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg
     9901 aatagagcac gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta
     9961 aatgaagcgt gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa
    10021 agtcgcgagc cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg
    10081 tactcggtgc atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt
    10141 caaggcgtgc catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt
    10201 caattggtta cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa
    10261 tgccgtcaga tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca
    10321 aagcaggcat cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac
    10381 gatgctgcag ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg
    10441 ttcttgcgcc attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat
    10501 tgatatctgg aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg
    10561 cggttcgaga attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat
    10621 tgtcatcatc gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa
    10681 cgtcgcctat tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca
    10741 ggttgcttga ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg
    10801 aatccgttct taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt
    10861 tgcctttcat ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt
    10921 tgtcattaaa tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca
    10981 gagcgcggcg tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata
    11041 tgggtcggcg atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa
    11101 catcgcgaat ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact
    11161 tctgcgggtg acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt
    11221 gtttcaatac aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca
    11281 ccaccgctcg cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc
    11341 tcagtgtcgc gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg
    11401 aggtggtcgt cgaagtatgg cgaaaacggg tgttttcttc atcgcgcaga atatggtgat
    11461 aggttagcac cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggcgagtc
    11521 gatcgccaat acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa
    11581 tcgggtagcg caaattgtcc gccagtaccc caaatggcgc actgcccgca ctcggcgcgt
    11641 tatagaccgg cgtatctttc caggtaatta agttctgatt actcagcggc ttgttgaggt
    11701 cacccaaacc gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga
    11761 taaacccttt accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg
    11821 caatgatttg cccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc
    11881 agatcgcagc attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg
    11941 cggtaaacag catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg
    12001 tgaaacaacg cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca
    12061 aattatgttt gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct
    12121 ccggcccacc cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc
    12181 aagccatcat ctgttcaatt aacggtaacg tgacaccaga aatcacttca caacgggagt
    12241 gtttatggct taataatgaa gccacgcggt acggtggcgc tccggctaaa tcggttaaga
    12301 agatgacacc ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca
    12361 tattagagct taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt
    12421 ttttctccag cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac
    12481 ctaacatcgc cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg
    12541 tgatagccgt caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg
    12601 caaaccacgt ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac
    12661 caccagtacg ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg
    12721 gaaaccactg atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg
    12781 caacaaagtc agggagtaaa acaccccgcc caaatacgtg agtggcgtta acacaaaggt
    12841 tggcaccagg ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag
    12901 ggagaatagc accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg
    12961 aaacggcaca aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc
    13021 cacgccaccg ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag
    13081 ctcttcaata ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc
    13141 attggtgatc accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc
    13201 atgcatatcg ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat
    13261 ggtgatgact ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt
    13321 cgcccagatg ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt
    13381 tcattaacca gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat
    13441 acctgaatgc cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc
    13501 acttcaactt ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt
    13561 aacggacttt tcggtgcgag atcgagaata aaggtttccg atttcagctt agccagcagc
    13621 gccttcatcg aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac
    13681 agcatttctg cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt
    13741 aaatccttca aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt
    13801 tcgtcgagaa tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc
    13861 ttcatcccgc cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagctgc
    13921 ttaagatatt tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc
    13981 tgattcacca caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc
    14041 agtcccaact gacgtttagc gttcacgaca tccttctcga gatcgtaacc aaatacgctg
    14101 acccgcccgg aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg
    14161 gccccgttcg gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctatccca
    14221 cgaagcgcct gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg
    14281 gtcataaatt tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg
    14341 agttgcccta tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag
    14401 acatagatac actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc
    14461 ccgggttttt tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt
    14521 ccgacagtcg cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc
    14581 accgtaatgt tgctaacctg gtcattcaca ctgacctgaa ctgcctttcc gtggttcagt
    14641 atgcagtgga tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg
    14701 gcgtacaagc cgcagttgaa aacccggaac tggggcttat caacaactgg ctgctgcata
    14761 tccgcgatat ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc
    14821 tggatacctt gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca
    14881 ttatgcaatc agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca
    14941 ttcacgacgg cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc
    15001 aacgttaccg tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat
    15061 gccatgccgg atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt
    15121 gccgatatac ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa
    15181 ctcatccggg atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg
    15241 tttatccagc agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg
    15301 cactttcgac agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc
    15361 acggatatct tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct
    15421 accgtagctg gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag
    15481 gtccgccata aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct
    15541 gtctttgtaa cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc
    15601 ttcggggatc attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa
    15661 atcacttcgc acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag
    15721 taaaactatt cattcgcgga atctgttaat aaagctcgaa ttgtgatgac gatcacacat
    15781 gttaaaacct ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat
    15841 ggcaattaac aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc
    15901 gctttgcggg ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg
    15961 gtactaccct atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa
    16021 acgtgccgcg ctttggctat acgccgccct gctgctcggc accatgattt ggggcgtctg
    16081 ggaagttggt ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg
    16141 catctggctg atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt
    16201 tgccgcactg gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa
    16261 cgatccgcag gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc
    16321 acccgttgcc gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc
    16381 acctctgaaa caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg
    16441 tactggcgat gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat
    16501 taaagtgggc gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc
    16561 cgccagcggc aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca
    16621 gcacgtaacc tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga
    16681 agtgatggcg gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc
    16741 gattaacgct gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct
    16801 gcaaagcaat atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat
    16861 caccgataaa accatcgtga tggcaggttc agttaccgat aacttctcaa cccgcgaaac
    16921 gtctggcgtg atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc
    16981 cggcgcgaaa gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc
    17041 aaactcgtgg gcgccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg
    17101 cgtgaccacg ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag
    17161 ctcgattctg gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca
    17221 ccacgacctg tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa
    17281 tggtcagaaa gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga
    17341 tcgtcgcaat ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc
    17401 gaaaggcgat tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa
    17461 agatttgagc ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt
    17521 gatgttccac cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct
    17581 ggtcttcccg ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg
    17641 tgaagtggca attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg
    17701 tccgggtaac ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat
    17761 tcagccacag tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg
    17821 tctgccgtgt aaacagccag catggggtta tatctcggcg ctggatctga aaaccaatga
    17881 agtggtgtgg aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt
    17941 accggtgcag ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa
    18001 cgtgctgttt atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg
    18061 tgaaaaactg tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga
    18121 agtgaatggc aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa
    18181 gatgggcgac tatattgtgg cttatgcgct gccggatgat gtgaaataac cataatgccc
    18241 ggagagatcc gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc
    18301 cccgtatctt catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg
    18361 ttgtgattaa acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc
    18421 cagcccgcgc gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgggtg
    18481 ccgtgaatat ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg
    18541 tactgccctt tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt
    18601 ttattggcat ggtggaaatc gaacttcccg ccatggttca tatgattcat attgccgtgc
    18661 cccatatggc ccatcatctg gctatgatcc atcccggcca tcgcctgatc gccatatttc
    18721 tccatcagca tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg
    18781 cgtaccgtca gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtctggcaaa
    18841 gcaccggagg cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac
    18901 ggcgcaatcg ccatccccat ctggctgacc ggtagcgtca ccaggtcaaa gggtttgttg
    18961 tcgttaacct ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc
    19021 ttcaccggtt caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg
    19081 ctggtggcga aattgagcga gcgggcatta cagccattaa gcaaacgcag gcgcagccaa
    19141 ccacgcgggg cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac
    19201 cagcccacgg cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat
    19261 tttttatcct gaacaatcac cggaacatca tcgatgcccc actgttttgg cagcattaat
    19321 ttcaggatct cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc
    19381 ccggtcttgc catgttgatg tggatggaac cagcaagtag cggcaggttg atcaacgttc
    19441 aacgtcaccg agcgcttgcc acctggcaga ataattccct gcgggccgcc gtcgacttca
    19501 cccggtactt ccagcccgtg ccagtgcaac gtcgtctctt ccgtcagttg gttatagata
    19561 tcaaccgtta ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta
    19621 tagccccagg tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac
    19681 tgaatgcgat tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc
    19741 gccgcaaaga ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa
    19801 tattttaaga aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa
    19861 atcaaacgtt gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa
    19921 tcgacaataa tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc
    19981 tggtagaagc aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgttt
    20041 gcgcaaacag ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag
    20101 tttttgtctt tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc
    20161 gcgcactggt ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg
    20221 acatgaaagc cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg
    20281 ctaaaaagtg caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat
    20341 ccctgatacc tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg
    20401 ttgcgccctt tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac
    20461 agtggcatga aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt
    20521 atcatgaaaa gaccgatcac caggatctga tcatttttga gaacgctgca tttggtcgcg
    20581 taatggcgct ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga
    20641 tgatgaccca tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg
    20701 gcggcgacgg tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga
    20761 tggtggaaat cgatgcgggt gtcgtgtcgt tctgccgtca gtatctgccc aaccataacg
    20821 ccggtagcta cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta
    20881 atcaaaccag ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg
    20941 gcgaaagtct gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg
    21001 gtatcttcgt cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagcc
    21061 atcgcaaact cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct
    21121 attacggcgg tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct
    21181 caaccgaaat tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc
    21241 cggcaatcca tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac
    21301 agccgtccta aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc
    21361 tgaccaaaag tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc
    21421 gcgacggtta tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc
    21481 tgtcagaaac ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg
    21541 aaccacaggg tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca
    21601 tcgacaaaac agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa
    21661 gtcatatttg cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc
    21721 gcgccgatat tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc
    21781 tgatccacca gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc
    21841 gcgacattaa cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta
    21901 tgtctgacga tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata
    21961 tcttccatac caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca
    22021 aaccggaaga tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa
    22081 tgcgcgagat ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc
    22141 aggctccgcc agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg
    22201 cagaaaatcc tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccctc
    22261 ttccatttca tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc
    22321 catccacagg gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc
    22381 gtgcgctgct tgttccactt catcaagcaa ggccaggttt tctttcacct cttcattaaa
    22441 ccagtgcccg accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc
    22501 gcgcagaaat tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca
    22561 ttgcgccatt gatgtaatta tcgcagcctt accggagaaa aacagcggaa cggcgatggt
    22621 ttagaaataa aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc
    22681 gcgtgcgctt acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta
    22741 ctgagaaagc tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg
    22801 cgcaacgtag gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc
    22861 cgccaattct gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt
    22921 acgggtagag gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca
    22981 cagggaacag ccagggatct cgatacgcgc accgctctta ccgaagacgc tatagtagcc
    23041 ttcttcggtc aactgcgcgg catccatacg ggttggcggt gccacccaca ggcgcgtcgg
    23101 caactggcct ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttggt
    23161 catgcaggaa ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg
    23221 tgcgtcgtcc gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat
    23281 gtcgatcact gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag
    23341 ccatttttcc atgccctgaa tacgacgttc cagggtacga cgatcgccgt aaccttccgc
    23401 gatcatccac ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt
    23461 cagcttgatg gtacaaccag cggcagaacg ctcggcggac gcatcggtca gttcaaatgc
    23521 ctgctcaact ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt
    23581 tttcttgcct ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc
    23641 gtgtaccaga tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac
    23701 ggattccggc atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga
    23761 acccgccggg aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac
    23821 ggtatccggc agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag
    23881 cgacacacca ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac
    23941 tggcttcgga tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc
    24001 caggcacgcc aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc
    24061 tacagaagtc attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc
    24121 acgacctacc attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc
    24181 ctgacggaac acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc
    24241 acgcccgata atcagcggga tacggccgcc agcacgcact tcatcaatca gcacgtcggt
    24301 tttcagttcg aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta
    24361 cgggtaaacg tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag
    24421 tgcacccgcg tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa
    24481 accaccgccg cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga
    24541 gttcgtggcg gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg
    24601 gaaacctttc tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc
    24661 tggctcaata ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg
    24721 gcgcgaccac gcatccggtg ccggagaaag gtcatcggtg ttagtttcgc cagtgacttt
    24781 gaagacagta acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc
    24841 cgcccaggac tgcataacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac
    24901 gtcatagaag ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc
    24961 cagtttggca tcatccagcg cgtcgatcag cgaatgaatg ttgtaaccac cctgcatggt
    25021 gcccagcagt tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttcgc
    25081 gatagcagcc aggaagcctg ctttgacata ggcggcttca tcgacgcctg ggggaacacg
    25141 gttggttaac agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc
    25201 tacaagtgcg gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg
    25261 ctcagctacg tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat
    25321 agtgcggcag gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc
    25381 agtaccgcaa cgggcagaat agcaggattt tgacgatgtg ttaatctgtt tacaaaaaag
    25441 caacataaaa aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa
    25501 acttcgttaa ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa
    25561 tttaaactac cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac
    25621 cattgcgatt tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg
    25681 ctggcgctaa tttgcagtgc cgggctttgt gccgcctcta ccgggctata tataaaaagc
    25741 cgcacagtgg aagcgcctgt ggaaccgcaa tcgacacaac agactgcgcc tgacatcacc
    25801 gcagttacgc ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc
    25861 gcattcagca ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct
    25921 ctgctttcac aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa
    25981 tggtcgcacg ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag
    26041 ccgtgggacg ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc
    26101 gaaaacccgc aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg
    26161 atggactcgg tacagcgatt gcggcaactg gcgcaacaaa ccggttcgct gaagtcatca
    26221 accgaacaaa aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg
    26281 gcacccgtca taccatccaa taccgtttta actgccagcc ccgtcattac agagcctgca
    26341 acaaccgtca tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac
    26401 ccaaccgtgg tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca
    26461 ccagccggag aaccgtttat tgacagcttt gtgcgcggat tcggctatag catgggtgtt
    26521 gctaccacgt acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat
    26581 catcatgacg atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg
    26641 caacacaacg gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag
    26701 catcttactg ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc
    26761 tatcatgatc aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagc
    26821 gccacgcagc tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt
    26881 cagcaacgaa cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca
    26941 cagcggttta atgaagctga aaactatggg agctatgacg acttccgcga cttcagccgt
    27001 cgccaaccac tgacccagca acaaaaggac gccgctcgtc agcgttatca gtcggcctcg
    27061 cctgagcagc gccaggcagt ccgcgagaaa atgcagacta acccacagat ccagcagcga
    27121 agagacgcag cgcgtgagcg tattcagtca gcctcgcctg agcagcgcca ggtgtttaag
    27181 gaaaaagtac agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtgtt
    27241 caatcagcat cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca
    27301 caacgtctaa acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta
    27361 cgcgaacgtc tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa
    27421 aaagcggcgt ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aacaattact
    27481 tcttcttcgc tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca
    27541 ggcccacaga ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat
    27601 cacaacccat ttcgattgcc aggccgattt cacccagcag ctcgccgccg ttggtaccga
    27661 caatcgcacc accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt
    27721 ctgcgcagtc ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat
    27781 agctgatgcc tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt
    27841 cggtataggc gatggacggg ataactttcg gatcgaagta gtgtttctta ccggcgataa
    27901 cttcagcggc aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccgacga
    27961 tatcgccgat agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga
    28021 aaccacggtc gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca
    28081 cacgaccaat cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc
    28141 cttccatcgt cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca
    28201 gcatcaggtt gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag
    28261 ctgccgggat aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt
    28321 atacggtgcc catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta
    28381 cttctttcag ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa
    28441 acggcagttg gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg
    28501 ttttgccgtt ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt
    28561 tgaccacttt gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga
    28621 tcactttctc tttccaggta cgaatcttgt caatatcggt tttcggttcg ccgaagacga
    28681 taccgtgttc agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt
    28741 tagaagggat acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga
    28801 ttacggtttc cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg
    28861 ccccaagtac cacgacctga gttttgattt cagtactcat catgacctct atatatttat
    28921 ctccggcggt catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc
    28981 ccagtcactt acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca
    29041 gaaatccata gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg
    29101 tattttacaa aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc
    29161 taataacctc aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt
    29221 ttacttacat caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac
    29281 gggcaccatc agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca
    29341 gacgcggcac gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga
    29401 ggatagccac ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga
    29461 tgctggagat ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg
    29521 ctttcttaga aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt
    29581 ctttgaatac cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt
    29641 atttcttcag ggtcagacgc tgaccgtctt ccgacaacga actgttgaag cgaggcatct
    29701 gctcaagagc tgcagcaacg gctttcatga tgaagacaac cggggtgatc ttcacatcca
    29761 gcttacgttt cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat
    29821 cggttttgtc gaagtgagta acatgcggga tcatcaccca gttacggctc aggttcgcac
    29881 cagaaatttt ctggatacgg cccagttcca cttcttcgat ttcaccaaac ttgctgaagt
    29941 ccaccttcgg ccacggcagc atgcctggga taccgccgcc agtcgccgcc ggagctgctt
    30001 ctgcacgttt gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt
    30061 tacggccagt gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga
    30121 tcagcggagt cgcgtgaacg taagcgtcgt tttctgcaaa ttcagatttg ccttccgctt
    30181 tcgcagccgg tgctgctgcc ggagcttcag cttttgctgc cggagccggc gctgccgctt
    30241 cctgtttcgc aggagccgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg
    30301 agccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg
    30361 gagccggaac ttccatagaa gctttgtcgc cttctacggt aatcagtgac tgttcagcgg
    30421 caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat
    30481 ccggaacgtt aacttctttc acgccagccg ctggtgcagg ggccgctgcc ggagccgctt
    30541 cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca
    30601 gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa
    30661 acggagccgg aacttccata gacgctttat cgccttctac ggtgatcagc gactgttcag
    30721 cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
    30781 tatccggaac gttaacgtct tttgccgctg cagccgctgg tgctgctgcc ggagctgctt
    30841 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
    30901 tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga
    30961 tacccgcctg cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg
    31021 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
    31081 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
    31141 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
    31201 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gctacaacca cgtaggaagc
    31261 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
    31321 acccagtacg cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
    31381 catatagtcg gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac
    31441 gcgcggagtt tccagcgggt gcagcatgtt ccagcgttca caatcctgac catctcgcgc
    31501 cagttcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
    31561 gatctctgcc gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt
    31621 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
    31681 accttccggc attgccggca tgtggtagtt ttcgttcagc gtggtgatgt agtagtaaac
    31741 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga cagcaacttc
    31801 gtaagcgtaa gccgggtcgt aagagataca gttcggaata gtcagcgact gaatgtggct
    31861 gtgaccatct tcgtgctgca gaccttcgcc gttcagggtg gtacgaccgg aagtaccgcc
    31921 gatcaggaag ccacgcgctt gctggtcgcc agccgcccag cacagatcgc caatacgctg
    31981 gaagccgaac atcgagtaat agatgtagaa cgggatcatc ggcagattgt tggtgctgta
    32041 agaggtcgcc gctgccagcc aggaacaacc tgcgcccagc tcgttgatcc cttcctgcag
    32101 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcggggtgta
    32161 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
    32221 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
    32281 tacgttcagg gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag
    32341 cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg
    32401 gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat gctcttcaga
    32461 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
    32521 gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat
    32581 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
    32641 tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
    32701 gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc
    32761 ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
    32821 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
    32881 tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt tccagccagc
    32941 accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
    33001 aagacgctgc aggttacagt tgataacgaa gactaggtta tccagttttt cacgggtagc
    33061 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
    33121 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
    33181 ctggtaaata gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc
    33241 cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
    33301 gttatccagc tgctcctgag tcagacgacc ttctaggaaa gcacgagcgt acacgcccgg
    33361 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
    33421 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc
    33481 gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat
    33541 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
    33601 ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt
    33661 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
    33721 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
    33781 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
    33841 gtcccattga actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc
    33901 ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
    33961 tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt
    34021 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
    34081 gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
    34141 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
    34201 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcagtg acagcaatct
    34261 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta
    34321 tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
    34381 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgactcag
    34441 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
    34501 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
    34561 cctcacgcaa ggagggacgg gagacgtcaa actgttttgc cagttcgcgt tccggtggga
    34621 gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca
    34681 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
    34741 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
    34801 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
    34861 gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac gtaggaaagg
    34921 agttttttaa cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt
    34981 tgtgattttt aatt
//
LOCUS       DAZFJF010000038        34904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFJF010000038 DAZFJF010000000
VERSION     DAZFJF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.038"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..554)
                     /locus_tag="ACHKAU_002046"
     CDS             complement(<1..554)
                     /locus_tag="ACHKAU_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO5531110.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWV"
     gene            complement(757..1158)
                     /gene="nikR"
                     /locus_tag="ACHKAU_002047"
     CDS             complement(757..1158)
                     /gene="nikR"
                     /locus_tag="ACHKAU_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO5531111.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1164..1970)
                     /gene="nikE"
                     /locus_tag="ACHKAU_002048"
     CDS             complement(1164..1970)
                     /gene="nikE"
                     /locus_tag="ACHKAU_002048"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO5531112.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1967..2731)
                     /gene="nikD"
                     /locus_tag="ACHKAU_002049"
     CDS             complement(1967..2731)
                     /gene="nikD"
                     /locus_tag="ACHKAU_002049"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO5531113.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2731..3564)
                     /gene="nikC"
                     /locus_tag="ACHKAU_002050"
     CDS             complement(2731..3564)
                     /gene="nikC"
                     /locus_tag="ACHKAU_002050"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO5531114.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3561..4505)
                     /gene="nikB"
                     /locus_tag="ACHKAU_002051"
     CDS             complement(3561..4505)
                     /gene="nikB"
                     /locus_tag="ACHKAU_002051"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO5531115.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLFVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4505..6079)
                     /gene="nikA"
                     /locus_tag="ACHKAU_002052"
     CDS             complement(4505..6079)
                     /gene="nikA"
                     /locus_tag="ACHKAU_002052"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5531116.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6190..6777)
                     /gene="acpT"
                     /locus_tag="ACHKAU_002053"
     CDS             complement(6190..6777)
                     /gene="acpT"
                     /locus_tag="ACHKAU_002053"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO5531117.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6832..7881)
                     /locus_tag="ACHKAU_002054"
     CDS             complement(6832..7881)
                     /locus_tag="ACHKAU_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5531118.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8013..9230
                     /locus_tag="ACHKAU_002055"
     CDS             8013..9230
                     /locus_tag="ACHKAU_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5531119.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9234..9791)
                     /gene="dcrB"
                     /locus_tag="ACHKAU_002056"
     CDS             complement(9234..9791)
                     /gene="dcrB"
                     /locus_tag="ACHKAU_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO5531120.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9864..10529)
                     /locus_tag="ACHKAU_002057"
     CDS             complement(9864..10529)
                     /locus_tag="ACHKAU_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO5531121.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10750..10995
                     /gene="tusA"
                     /locus_tag="ACHKAU_002058"
     CDS             10750..10995
                     /gene="tusA"
                     /locus_tag="ACHKAU_002058"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO5531122.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(11097..13295)
                     /gene="zntA"
                     /locus_tag="ACHKAU_002059"
     CDS             complement(11097..13295)
                     /gene="zntA"
                     /locus_tag="ACHKAU_002059"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO5531123.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13369..13995)
                     /locus_tag="ACHKAU_002060"
     CDS             complement(13369..13995)
                     /locus_tag="ACHKAU_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO5531124.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14136..14495
                     /locus_tag="ACHKAU_002061"
     CDS             14136..14495
                     /locus_tag="ACHKAU_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO5531125.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14498..14767)
                     /locus_tag="ACHKAU_002062"
     CDS             complement(14498..14767)
                     /locus_tag="ACHKAU_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO5531126.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14757..15353)
                     /gene="rsmD"
                     /locus_tag="ACHKAU_002063"
     CDS             complement(14757..15353)
                     /gene="rsmD"
                     /locus_tag="ACHKAU_002063"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO5531127.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15503..16999
                     /gene="ftsY"
                     /locus_tag="ACHKAU_002064"
     CDS             15503..16999
                     /gene="ftsY"
                     /locus_tag="ACHKAU_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO5531128.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            17002..17670
                     /gene="ftsE"
                     /locus_tag="ACHKAU_002065"
     CDS             17002..17670
                     /gene="ftsE"
                     /locus_tag="ACHKAU_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO5531129.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17663..18721
                     /gene="ftsX"
                     /locus_tag="ACHKAU_002066"
     CDS             17663..18721
                     /gene="ftsX"
                     /locus_tag="ACHKAU_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO5531130.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18966..19820
                     /gene="rpoH"
                     /locus_tag="ACHKAU_002067"
     CDS             18966..19820
                     /gene="rpoH"
                     /locus_tag="ACHKAU_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO5531131.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20091..21194
                     /gene="livJ"
                     /locus_tag="ACHKAU_002068"
     CDS             20091..21194
                     /gene="livJ"
                     /locus_tag="ACHKAU_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO5531132.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21393..21776)
                     /gene="panM"
                     /locus_tag="ACHKAU_002069"
     CDS             complement(21393..21776)
                     /gene="panM"
                     /locus_tag="ACHKAU_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO5531133.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            22200..23309
                     /gene="livK"
                     /locus_tag="ACHKAU_002070"
     CDS             22200..23309
                     /gene="livK"
                     /locus_tag="ACHKAU_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO5531134.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23357..24283
                     /gene="livH"
                     /locus_tag="ACHKAU_002071"
     CDS             23357..24283
                     /gene="livH"
                     /locus_tag="ACHKAU_002071"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO5531135.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            24280..25557
                     /gene="livM"
                     /locus_tag="ACHKAU_002072"
     CDS             24280..25557
                     /gene="livM"
                     /locus_tag="ACHKAU_002072"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO5531136.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25554..26321
                     /gene="livG"
                     /locus_tag="ACHKAU_002073"
     CDS             25554..26321
                     /gene="livG"
                     /locus_tag="ACHKAU_002073"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO5531137.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            26323..27036
                     /gene="livF"
                     /locus_tag="ACHKAU_002074"
     CDS             26323..27036
                     /gene="livF"
                     /locus_tag="ACHKAU_002074"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO5531138.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            27432..28748
                     /gene="ugpB"
                     /locus_tag="ACHKAU_002075"
     CDS             27432..28748
                     /gene="ugpB"
                     /locus_tag="ACHKAU_002075"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO5531139.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28846..29733
                     /gene="ugpA"
                     /locus_tag="ACHKAU_002076"
     CDS             28846..29733
                     /gene="ugpA"
                     /locus_tag="ACHKAU_002076"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO5531140.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29730..30575
                     /gene="ugpE"
                     /locus_tag="ACHKAU_002077"
     CDS             29730..30575
                     /gene="ugpE"
                     /locus_tag="ACHKAU_002077"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO5531141.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30577..31647
                     /gene="ugpC"
                     /locus_tag="ACHKAU_002078"
     CDS             30577..31647
                     /gene="ugpC"
                     /locus_tag="ACHKAU_002078"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO5531142.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            31644..32387
                     /gene="ugpQ"
                     /locus_tag="ACHKAU_002079"
     CDS             31644..32387
                     /gene="ugpQ"
                     /locus_tag="ACHKAU_002079"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5531143.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32374..32814)
                     /locus_tag="ACHKAU_002080"
     CDS             complement(32374..32814)
                     /locus_tag="ACHKAU_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO5531144.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            32934..34676
                     /gene="ggt"
                     /locus_tag="ACHKAU_002081"
     CDS             32934..34676
                     /gene="ggt"
                     /locus_tag="ACHKAU_002081"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO5531145.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(34714..>34904)
                     /locus_tag="ACHKAU_002082"
     CDS             complement(34714..>34904)
                     /locus_tag="ACHKAU_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YrhB domain-containing protein"
                     /protein_id="HFO5531146.1"
                     /translation="GWYFCFEAREFLETGDEAARLAGNAPFIIDKDSGEIHSLGTAKP
                     LEEYLQDYEIKKATFGLP"
BASE COUNT         8075 a         9119 c         9789 g         7921 t
ORIGIN      
        1 acccaccacg gcccctgcgg actgtttgtc gccagataac gatgcggact caggcgaagc
       61 tcttccggca gcgcctgcca gagtgcggcc agccggtgac cttcatccag tttcgccaca
      121 ccgccgcgca ccagccagag tgattcgctg cggctgtaac cgtcctcacc gggaaacagg
      181 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      241 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      301 gtttttgtcc ggtaactgct gtaggtgcgg gagagaatga acggcagcgg gccgggcagg
      361 gcgatgtcgg tttcaccggg aaggaccttt gcacccagca gcggattgac cggatggccg
      421 gacgtcaccc cgccggggca caccgaacag gccacgccgg tgggggcacc aatacgtacc
      481 ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcaccctg acgcgccgcc
      541 ggttttccgc tcatcgtaaa atccttttct attattttaa tgacagtatc ttcatacgaa
      601 aaagcgaaga aaaacttgtg gtcacaatga attgaagatt tttaggaata tatttagaac
      661 gttacaaaag agaaggttcg gatagggtat ttagagagga atcaggtgtg ctagtggccc
      721 tttgttgatc gttcaccaaa ggacccaata atagactcaa tcttccttcg gcaagcactg
      781 caaatgcccg tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc
      841 gcccatatca cctttcagca cggcgatttc cagacagtcg tcgtgattga tatgcacatg
      901 cagcgtggcg acggagagat cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa
      961 gtcgcgtttt tcgtgttcat acacatagga gagcaccgcg aaaccttgcg tgccgtgctg
     1021 ctgagtggcc tcctgggcca gagcgctacg cagaatatcg cggatagctt cggaacgatt
     1081 gttataacca cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt
     1141 gatggtgact cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat
     1201 gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct
     1261 cccaccacct gggtttcgac gatttgtccg ttgtccatca ccatcacccg ctgacagaag
     1321 cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa ctgttgttgt
     1381 agctttttca gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct
     1441 tcatccagaa tcagtagttt cggttcgacc gccagcgcgc gagccaggca gacgcgctgg
     1501 agctggccgc cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc
     1561 gccttcagca tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg
     1621 cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc gctgatggag
     1681 tcctgaaata ccatctgaat atcgcggcgg aacgctttac gctgggcgcg attgagtttc
     1741 gccagcggtt cgccacgcca gctaatattc ccctgcgcgg gcgattctaa acccaccagc
     1801 aaccgcgcga gggtactttt cccgcagccg ctgcgcccca gcagggcgac ggtttcgccg
     1861 cttttcaggg tcagggaaac gttattcagc accgcctgat gttgatgttt tccgttaaat
     1921 ccaccgtgcg catagtgatg ggaaaggccg gagatgttaa gtaaagtcat gatgccagct
     1981 ccataccgta gagggcgaga tgagcggaaa ccaggctgcg cgtcactgta tgtttggggg
     2041 cgttaaacag cgtttctaca tcgccctgtt cgacaatctt accgtcagac atcaccgcca
     2101 cgtcatccgc cagacgcgcc acaacgccca tatcatgggt caccagcagc attcccggcg
     2161 cttgtttttg cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga
     2221 ggtcggtggt cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa
     2281 tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg
     2341 cggcgttttc cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt
     2401 tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgcagt ggattaaagg
     2461 cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg caaggcgaaa
     2521 ccggtttgcc atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc
     2581 ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca
     2641 cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg
     2701 cgatattacg tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg
     2761 gtccagatga tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag
     2821 cgccagcccc ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt
     2881 aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc
     2941 cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat
     3001 caccgcgcct gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga
     3061 cgccagcaca aactcgcgtt ggcgtagtga aatcaccagg ctgcgcacca tgcgtgcata
     3121 ccacgcccag tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac
     3181 gccaaccata aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg
     3241 catggtggcc tgatcaacgc gcccgccaat caacccggcg ctgccgccaa taaccagccc
     3301 taatgtcagc accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgctgt
     3361 catcagccgc gagaaaatat cgcgacctaa gtgatcggtg cccagccagt gctgcgcatc
     3421 cggcgaaagc aggcgcgacg gcaaatcaat cgcctggggg tcatacggca gccaccactg
     3481 gctggtgagc gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca
     3541 gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc
     3601 agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc
     3661 agcgtaaagc actggatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc
     3721 ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg tcccgccaat cagttcgccg
     3781 atatgcatcc ccacggcggt gatcatcggc agcgaggcat tgcgcaggat gtggcgacgt
     3841 tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccagg tgacgtgacg ctgaccggcg
     3901 acgtccagca tactggcgcg cagtaaacgc gcgttaatcg ccagcgacat aaaggcaatg
     3961 gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg tagccattgc
     4021 agatacaccg aaaacgccat caccagtaaa aacgccagcc agaagttagg catcgacacg
     4081 ccaagaaacg cgatgaaacg tacggcgaaa tccggcagac ggtcgcgatg gcgcgccgcc
     4141 cagataccga gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc
     4201 agcgtggcgg gcaggaagtt cagcatatcg tccagtaccg ggcgttggct ggcgaatgag
     4261 ataccaaagt caagatgcag cgccttccac aaccaggtac cgtactggac gaacagcggc
     4321 tgatccagcc cgagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta
     4381 gataaacgca gataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg
     4441 atcaccgagg cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc
     4501 aacattaagg tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt
     4561 aggggatgtt acccagctcc ggttttgata ccaccatcat tgagatgtaa ctgataggca
     4621 gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg atacagcgcc tgacgttgcg
     4681 tttcgtcatg ggtcgccagc acttcgccga tctctttatc aatcagcggt ttgtcggcta
     4741 atccttgctg tgcctggaag tcagcgtgtg acggtacgcg cattgaactg aggaaggcgt
     4801 gtggatcata tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct
     4861 gacgagcata gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc
     4921 gcatatcagc ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg
     4981 acagttcaat gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg
     5041 tccaaccggc tttttccagc aacgctttcg ctttctgcgg atcgtactgg cgcggtttca
     5101 ggccgaggtt ggcgtagggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc
     5161 catacaacgc gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta
     5221 ctgccagctc gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg
     5281 gctgtgacag ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg
     5341 gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc
     5401 gggtagtcgg gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc
     5461 agtagttttc gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc
     5521 acggtccggt gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg
     5581 agggagcgat aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg
     5641 cgcttttcag ggtaatttgc agctctgttt tactgagtgc tttaacatca acaatctggt
     5701 ttgccagctc cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg
     5761 ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga
     5821 aggtccaggt tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg
     5881 aaccgtctgc ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt
     5941 taggcgtgta aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtggtgattt
     6001 catctggtgc agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc
     6061 ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa tagatggcgg aaataagtat
     6121 gacgatttta agtattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg
     6181 gtgggcggat cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg
     6241 agtaggggtg cagatcgcca ggcttaaatt ttcaagctga caatggctga ctgacagcga
     6301 ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac
     6361 gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac
     6421 ggcgtccatc tcagcgtgtt ccccgaggct gaatacggcg ttcgccagcc agtgccagtt
     6481 ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg ccttcatcac tcaacagcag
     6541 ggcgatatcg tcaccgctat ggcttaagtt gaaccatagc ggcatttccg gcgcaaatgc
     6601 aggtttgcct tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa
     6661 tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc
     6721 cggtggcagt ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc
     6781 agggccaacg tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg
     6841 taaccgactt ttcggtctgc ctggccccag taaaatcgcc agtttgctac cgcctttggt
     6901 ggtttccatc cagattttac acacactggt taatggcacc gaaagtagca tccctaccgg
     6961 gccgagcagc catccccaaa ttaacaatga aagaaatacc accatggtgg acatccccag
     7021 gcgatggccc atcatccgtg gttctaaaat attgccgatg accatatgga ccactaaaaa
     7081 caatgcgccg accagaatac attcgtaaac accattaaac agcagcacct gaatcattgg
     7141 cggtacggcg gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac
     7201 tgcccacatc agcgcaaact gcacccccat cagctccagc cccagccaga cgatgacacc
     7261 tgtccataaa ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg
     7321 gtgtaatcct gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac
     7381 gtggcgcact tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat
     7441 tgccccggaa agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat
     7501 ccgctgcagc attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa
     7561 tttaaaaagt ttgcgcgtca actccttatt aaacttcggc agcatagaga taaattcgtt
     7621 aaacgatgcc gccagtacgc cgaccagcgc agttagtgcg atcagcatca ccaccaccac
     7681 aatcgtaatg gcaatggggc gttgtactcc ccgacgaata aaccaggtga cgagcgggtt
     7741 gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg
     7801 aatgcccgcg aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt
     7861 atcgggttga ggggtttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg
     7921 agtcacgcca gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa
     7981 tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc
     8041 taaacggatt gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct
     8101 acctcaccat cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct
     8161 ttagcgcttt ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc
     8221 gtcctcatgc cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt
     8281 tatgcggctg ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc
     8341 ccgtcatcag cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt
     8401 ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc
     8461 gagtgatttc gtggaacggc attgtcactt acggggcgat ggcgatgggt gcgccgttag
     8521 gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg
     8581 cgctggtggc cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac
     8641 cgctgccgtt tcgcgcggtg cttgggcgcg tctggctgta cggtatggcg ctggcactgg
     8701 cttccgccgg atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt
     8761 gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt
     8821 tattccctaa cggcattaac cgtatcggtg gcttaaacgt agcgatgatt tgctttagcg
     8881 ttgagataat cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaaatcg
     8941 gcgtcttact ggcgggggcc gggttttcgc tggtgttccc ggcattgggt gtagtggcgg
     9001 taaaagcggt tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg
     9061 atttatcgct tggcgtgact ggaccactgg ctgggctggt gatgagctgg gcgggcgtac
     9121 cggtgattta tctggcggcg gcgggactgg tcacaatcgc gttattactg acgtggcgat
     9181 taaaaaaacg gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga
     9241 ataaccagcg tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagct
     9301 ggcagagtaa tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag
     9361 tacgccgtct ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc
     9421 agctcaatgg ctttattggt taccacttgc agctgcggat cgcggctacg ttgctgatct
     9481 tccagacgct tcgccagcac cgccagatct tcttttggat catcgcccat gatgacgatg
     9541 actgctttct gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc
     9601 ttaccgctct ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg
     9661 ttgacgggat tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg
     9721 tctttatcat cacaggccgc aagccccata accagcaggc caattccgac atatttaacc
     9781 agattgcgca ttgacttctt cctttcgata aacggccata acggctcatt catccatctt
     9841 atcacaactc tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct
     9901 gccagtcttt tcaacagcat attgagtaat acgccataca ttggcaggaa gaaaacgata
     9961 ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata
    10021 aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta
    10081 ccgaacagtg tggacgctgt cggtgccagc caccagcggc gactttgacg caggcggtta
    10141 aaaacgtgca cgtcgaggat ttgccccagc gcgtaggcca tgaaactggc ggtggcgata
    10201 cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc cctgccatga acccatatag
    10261 aacagcgacg agatgacata ggagattaat aacgcaggga tcattaccgc gaagataatg
    10321 cgtcgggcca gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag aaaaataaac
    10381 ggaaagctaa acgcgcccca ggtggtatgg aaaccaaaaa tggagacggg aagctgaacc
    10441 agatagttac tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta
    10501 tagcgttgag tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga
    10561 gggaacccaa tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt
    10621 tcgagccgcc gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc
    10681 gttaacctgg tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat
    10741 gagaagaaaa tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg
    10801 cgctgcccgg aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcccggcgaa
    10861 acgttgctga ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc
    10921 tttatggaac acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt
    10981 cgtaaaggcg gttgatgggg gctgattggc ttcgatgccg ccttttcccc tcaccctaac
    11041 cctctcccca gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat
    11101 ctcctgcgca acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc
    11161 agcaccgcca gccatagtcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc
    11221 agtcccagcg caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca
    11281 atcatttgca ccaggccgcg caggtggtta tgggttaatg ctgcatcggc ggtttccagc
    11341 gccacgtctg tgccgctgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg
    11401 tcgttaatac catcaccgac catcgccagc ggcgcatgtt gattcagctt ggtcaccgct
    11461 ttgactttct cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt
    11521 gccgccgctg cgcgtggatt atcgccggtg aggatcactc ctttaactcc cagcgcgttc
    11581 agttcactga tggcagttgc agcgtcggcg cgcagggtat cctgcaatgc aatgataccc
    11641 agcacgtcat cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta
    11701 atcaaaccag caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca
    11761 ccgttaacct gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggtggga
    11821 atggcgagtt cagcaacctg tgcttctcgc acgatggcct gcgccagtgg atgcgtcgca
    11881 ccttgctcga ccgccgctgc cagtgtcagc agttcagatt cactaatacc cgttgccgga
    11941 tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc
    12001 tgggtaacac gacctagttg ttccagcgcc gctccgcctt taattaacgc cccacgacgc
    12061 gctgctgccg ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa cgcacacggg
    12121 cagccaatca gcagcagcgt caggccttta taaatccact cttgccagct ggcggcaaac
    12181 agcagcggtg gcaccagcgt caccagcaga gcgacggcca taatcgcggg cgtatagata
    12241 cggctgaaac ggtcgataaa ccgctcaatg ggtgcgcgac gctcttcggc ttcttcgatc
    12301 agtttcagaa tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc
    12361 agacggtcta cgctggtggc tccagcaggg actttatcgc ccgttgcgcg ctccaccgga
    12421 atagattcgc cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg
    12481 tcggcaggca aacgtccacc tgcggcgact tcaatcacat cgccagggcg caggctgtta
    12541 atcgccacct cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc
    12601 attaacgcgc tgaccccttg acgtgcgcgg ctggcggccc agccttccag tcgttcgcca
    12661 atcaaaaaca gcagcaacac catcgcagct tcagccgttg cgccaataaa aagcgcacca
    12721 atagcggcta cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcaaccgt
    12781 aatgcctgac gagcaatcgg gtacagccca accagtgtgg tcgcgataaa cgccagttgc
    12841 ccgaacggat gattgaactg ctccagaccc cagctgattg ccatcatcac gattagcgta
    12901 atcagcggca gattctcttt caggcgtgat gcttgcggtt cgtcggcgga ctgttcatcg
    12961 cgcagggagt agcccgcttt ttgcaccgca gattcaacct gggcacggat gtcattgtcg
    13021 gcatcaacca ccagtttttc ggtggcgaac aacacctgta cctgattcac gcctgcaagc
    13081 tggcgcacgg cattttctac cttgcgcgca caggcggcgc agtccatgcc gctgactttc
    13141 cagctatagc gggtgccgga gacgttttca gagagagttg gcgtgctgga acatgcgccg
    13201 tcgcagcaac agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga
    13261 ggggctttct tgccgtgatt gtcaggagtc gacatggcat cctccggtta agttttttct
    13321 cattaaccga aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag
    13381 cgagcggacg atcaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc
    13441 acggaagcgt cggcgatagt gactacccag ccagacaaag ttactgataa acagcaacga
    13501 agcgccgaca aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc
    13561 cagccacacc atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc
    13621 cagccgcgtc cagataatcg ccagtaacag cgcgcccaga accagcagca ccagtggcag
    13681 aggccagaag aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga
    13741 gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc
    13801 gcccagcagt gaggcgcaca gccccgccag caccagatag ctaatggcgt cgaacatcgg
    13861 cgcttgccag gccagtaaca gcaggagaag aagggttaat ggtttaaata cccagcgttg
    13921 ccaggttggc ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat
    13981 aaacgaccaa agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac
    14041 ggtcttgctt ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt
    14101 atgcttaatt ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta
    14161 ttgttatcat tggcttaatc atcgtcgccg catcgtttcg ttttatgcaa caacgacggg
    14221 aaaaagctga taatgatatg gcaccactcc agcaaaagct ggtgttggtg agcaacaagc
    14281 gggaaaaacc gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca
    14341 gtatgcgcta tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc
    14401 tcgacgccca gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag
    14461 gaacgcgctt tgtcagcttt gtaggcgaac aataaggtta ttttttaact ttaaatttct
    14521 tctgccagac cagcagttca aacacgccga aaaggaaaat ccgtaccttt tcggcggtgg
    14581 tcatctgcgg gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca
    14641 tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt cagcggacgt gggaagggat
    14701 gcaccaggtt gaggattaaa aatccccaaa cacagagcat taacaaacga ccaatattaa
    14761 tcagcatcac tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc
    14821 actttttccc gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg
    14881 ctttcgacat aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc
    14941 gtctcttcta acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgta
    15001 cctttttgcg ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt
    15061 agtgtcgcca gattcttaat taactgctga gaaaccgcgc gatccatctc aatcaacgtt
    15121 gcccccgcag catagcgcga caacgcttcc agccccagcg cgccgcttcc ggcgaagcaa
    15181 tccagacatt gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg
    15241 cggtcggtgg tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac
    15301 tgcccgccaa taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat
    15361 tgctcaatcc gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata
    15421 gactatttca tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca
    15481 acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct
    15541 gggctttggt caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc
    15601 ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag gcttcggaac aagccgttga
    15661 agagcagccg caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga
    15721 agtcactgaa caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca
    15781 gccggaaccg gtcgcagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc
    15841 gttgccagaa gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga
    15901 aaccgtagag attgtcgaag cggcagaaga agaagcggct aaagaagaaa tcaccgacga
    15961 agagctggaa gcacaggcgc tggctgccga agcggcagaa gaggcggtga tggtagttcc
    16021 tccggcagaa gaagagcagc cggtggaaga aattgcccag gagcaagaaa aaccgaccaa
    16081 agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc
    16141 cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga
    16201 agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct
    16261 gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa
    16321 agaagagatg ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac
    16381 gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaactacga cgattggtaa
    16441 gctggcacgt cagtttgagc agcagggtaa atcggtgatg ctggcggcgg gcgatacttt
    16501 ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat
    16561 tgcccagcat accggtgcgg attccgcctc tgttatcttc gacgccattc aggcagctaa
    16621 agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca
    16681 cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc
    16741 gcatgaagtt atgctgacta ttgatgccag taccgggcag aatgcggtaa gccaggccaa
    16801 actgttccac gaagccgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc
    16861 gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg
    16921 tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact
    16981 ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt
    17041 gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg
    17101 accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg
    17161 cccagcgccg ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa
    17221 gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac
    17281 cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat
    17341 attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac
    17401 ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg
    17461 aataagcccg cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg
    17521 gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca
    17581 acgcacgaca tcaacctgat ttcacggcgt tcctatcgca tgctcaccct gagcgatggt
    17641 cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt
    17701 tggcgggcgt cttgatcgct tccgtaaatc ggtcggcggc tctggcgacg gcggtcgtaa
    17761 cgccccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgtaaaacca acgttttcaa
    17821 cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc
    17881 cacgttttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat
    17941 ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt
    18001 ttacttgcaa aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc
    18061 cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg
    18121 taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt
    18181 ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga
    18241 tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg
    18301 tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt
    18361 ggcggcagtg ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga
    18421 ctccatcaac gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct
    18481 gtatggtggc gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat
    18541 tctgatgctg cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt
    18601 tgatatcaat ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat
    18661 tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata
    18721 ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt
    18781 tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt
    18841 gcattgaact tgtggataaa atcacggtct gataaaacag tgaatgataa cctcgttgct
    18901 cttaagctct ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga
    18961 tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct
    19021 acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg
    19081 aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc
    19141 ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga
    19201 ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg
    19261 tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc
    19321 gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc
    19381 tgcgtaaaac caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc
    19441 gtgaactggg cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg
    19501 acatgacctt tgacctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc
    19561 tctatctgca ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag
    19621 agcaggcggc aaaccgtctg accgacgcga tgcagggtct ggacgaacgc agccaggaca
    19681 tcatccgtgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc
    19741 gttacggtgt ttccgctgaa cgtgtgcgcc aactggaaaa gaacgcgatg aaaaaacttc
    19801 gcgccgctat tgaagcgtaa tttccgctat taagcagaga accctggatg agagtccggg
    19861 gtttttgttt tttgggcctc tacaataatc aattccccct ccggcaaaac gtcaatcccc
    19921 acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa tggggtatgt
    19981 tttagcagag tatgctgcta aagcacgggt agtcatgcac aaaacgaaat aaagtgctga
    20041 atcacaacac cacaacacac gtaataacca gaagaatggg gattctcagg atgaatataa
    20101 agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag
    20161 aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc
    20221 aggagtttac cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag
    20281 gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg
    20341 tggcgaacaa agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat
    20401 caacgcagcc tgcgtctgat atctacgaag acgaaggcat tttaatgatc accccagcgg
    20461 caaccgcgcc ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg
    20521 actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta
    20581 ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcacgagcg gtgcaggacg
    20641 gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag
    20701 atttctctac gctggtggcg cgtctgaaga aagagaatat cgacttcgtt tactacggcg
    20761 gttaccaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc
    20821 agtttatggg gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag
    20881 cggaagggct gctggtgacc aagccgaaga actacgatca ggttccggca aacaaaccca
    20941 ttgttgacgc gatcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct
    21001 acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgagatcg
    21061 ccaaatacct gaaagcgaac tccgtggata ccgtaatggg accgctgacg tgggatgaga
    21121 aaggcgatct gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga
    21181 ccgatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag
    21241 tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctacaag atccaaagaa
    21301 atcagtaaat tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc
    21361 gttggtcatc acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc
    21421 gccgtaaacc ccagcgcctg cataaacgcc gtcatcacac cgcgatcttc caagcctgca
    21481 tccgccatcc accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat
    21541 tgccccacac cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg
    21601 gtgccgctta aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag
    21661 atacggtggt tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc
    21721 aggtcaatcc ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt
    21781 aacccgtgta aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc
    21841 tcttttttat ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa
    21901 acattagaga atgaaaaata cccagcataa tcccctgaat ggtagtgaat tattccgccc
    21961 tttgtgccgt tattttatgc tgacaaaggc acttttttct gtttatctat caataaattc
    22021 agaatattat ctgttcttaa tcgactgaaa aatagggatt ttaatcacta ttatcacaaa
    22081 atactgcgct aaacccttaa tcagacaggc aaaaacagtg cagtataaaa aaagaacagt
    22141 ctgatttgtt aacacataaa aacaaagcaa cacaacatca cgaatgggga tttttgacta
    22201 tgaaacggaa tgcgaaaact atcatcgcag ggatgattgc actggcaatt tcacacaccg
    22261 ctatggctga cgatattaaa gtcgccgttg tcggcgcgat gtccggcccg attgcccagt
    22321 ggggcgatat ggaatttaac ggcgcgcgtc aggcgattaa agacattaat gccaaagggg
    22381 gaattaaggg cgacaagctg gttggcgtgg aatatgacga cgcctgcgac ccgaaacaag
    22441 ccgttgcggt cgccaacaaa atcgttaatg acggcattaa atacgttatt ggtcatctgt
    22501 gttcttcttc tacccagcct gcgtcagata tctatgaaga cgaaggtatt ctgatgatct
    22561 cgccgggagc gaccaacccg gagctgaccc aacgcggtta tcaacacatt atgcgtactg
    22621 ccgggctgga ctcttcccag gggccaacgg cggcaaaata cattcttgag acggtgaagc
    22681 cccagcgcat cgccatcatt cacgacaaac aacaatatgg cgaagggctg gcacgttcgg
    22741 tgcaggacgg gctgaaagct gctaacgcca atgttgtttt cttcgacggc attaccgctg
    22801 gcgagaaaga tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt
    22861 actacggcgg ttactacccg gaaatggggc agatgctgcg ccaggcccgt tccgttggcc
    22921 tgaaaaccca gtttatgggg ccggaaggtg tgggtaatgc gtcgctgtcg aatattgctg
    22981 gcgatgctgc cgaaggcatg ttggtcacca tgccaaaacg ctatgaccag gatccggcaa
    23041 accagggcat cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg ccttatgtct
    23101 ggatcactta cgcggcggtg caatctctgg caacagccct tgagcgtacc ggcagcgatg
    23161 agccgctggc gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg attgggccgc
    23221 tgaactggga tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc cagtggcacg
    23281 ccgacggttc atccacggca gccaagtgat catcccaccg cccgtaaaat gcgggcgggt
    23341 ttagaaaggt taccttatgt ctgagcagtt tttgtatttc ttgcagcaga tgtttaacgg
    23401 cgtcacgctg ggcagtacct acgcgctgat agccatcggc tacaccatgg tttacggcat
    23461 tatcggcatg ataaacttcg cccacggcga ggtttatatg attggcagct acgtctcatt
    23521 tatgatcatc gccgcgctga tgatgatggg cattgatacc ggctggctgc tggtggcggc
    23581 gggattcgtc ggcgcaatcg tcattgccag cgcctacggc tggagtatcg aacgggtggc
    23641 ctatcgcccg gtgcgtaact ctaagcgcct gattgcgctc atctcggcaa tcggtatgtc
    23701 catcttcctg caaaactacg tcagcctgac cgaaggttcg cgtgacgtgg cgctgccgag
    23761 cctgtttaac ggtcagtggg tggtgggcca tagcgaaaac ttctctgcct ctattactac
    23821 catgcaggcg gtgatctgga ttgtcacctt cctcgccatg ctggcgctga cgattttcat
    23881 tcgctattcc cgtatggggc gcgcgtgtcg tgcctgcgcg gaagatctga aaatggcgag
    23941 tctgcttggc attaacaccg accgggtgat tgcgctgacc tttgtgattg gcgcggcgat
    24001 ggcggcggtg gcgggtgtgc tgctcggtca gttctacggc gtaattaacc cctacatcgg
    24061 ctttatggcc gggatgaaag cctttaccgc tgcggtgctc ggtgggattg gcagcattcc
    24121 aggggcgatg attggtgggc tgatcctggg gattgcggag gcgctctctt ctgcctatct
    24181 gagtacggaa tataaggatg tggtttcatt tgccctgctg attctggtgc tgctggtgat
    24241 gccgaccggt attctgggtc gcccggaggt agagaaagta tgaaaccgat gcatattgca
    24301 atggcgctgc tctctgccgc gatgtttttt gtcctggcgg gcgtctttat gggcgtgcaa
    24361 ctggagctgg atggcaccaa actggtggtc gacacggctt cagacatccg ttggcagtgg
    24421 gtgtttatcg gcacggcggt ggtctttttc ttccagcttt tgcgaccggc tttccagaaa
    24481 gggctaaaaa gcgtttccgg accgaagttt attctgcccg cgattgatgg ctccacggtg
    24541 aagcagaaac tattcctcgt ggcgctgctg gtgcttgcgg tggcgtggcc gtttatggtg
    24601 tcgcgtggga cggtggatat cgccaccctg accatgatct acattatcct cggcctcggg
    24661 ctgaacgtgg ttgttggtct ttctggtctg ctggtgctgg ggtacggcgg cttttacgcc
    24721 atcggcgctt acacttttgc gctgctcaat cactattacg gcctgggctt ctggacctgt
    24781 ctgccgattg ccgggttaat ggcagcggcg gccggcttcc tgctcggctt cccggtgctt
    24841 cgcttgcgcg gcgactatct ggcgatcgtt accctcggtt tcggcgaaat tgtgcgcata
    24901 ttgctgctca ataacaccga aattaccggc ggcccgaacg gaatcagtca gatcccgaaa
    24961 ccgacactct tcggactcga gttcagccgt accgctcgtg aaggcggctg ggacacgttc
    25021 agtaatttct ttggcctgaa atacgatccc tccgatcgtg tcatcttcct ctacctggtg
    25081 gcgttgctgc tggtggtgct aagcctgttt gtcattaacc gcctgctgcg gatgccgctg
    25141 gggcgtgcgt gggaagcgtt gcgtgaagac gagatcgcct gccgttcgct gggcttaagc
    25201 ccgcgtcgta tcaagctgac tgcctttacc ataagtgccg cgtttgccgg ttttgccgga
    25261 acgctgtttg ctgcgcgcca gggctttgtc agcccggaat ccttcacctt tgctgaatcg
    25321 gcgtttgtac tggcgatagt ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg
    25381 gcggcaattt tgctggtggt atcgcgcgaa ttgatgcgtg atttcaacga atacagcatg
    25441 ttaatgctcg gtggtttgat ggtgctgatg atgatctggc gtccgcaggg cttgctgccc
    25501 atgacgcgcc cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc
    25561 agccattatt atctgttaac ggtctgatga tgcgcttcgg cggcttgctg gcggtgaaca
    25621 acgtcaatct tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg
    25681 gaaaaaccac ggtttttaac tgcctgaccg gattctacaa acccaccggc ggcactattt
    25741 tactgcgtga tcagcatctg gaaggtttac cggggcagca aattgcccgc atgggcgtgg
    25801 tgcgtacctt ccagcatgtg cgtctgttcc gtgaaatgac ggtgattgaa aacctgctgg
    25861 tggcgcagca tcaacaactg aaaaccgggc tgttctctgg cctgttgaaa acgccttctt
    25921 tccgtcgcgc ccagagcgaa gcgctcgacc gcgccgccac ctggcttgag cgcattggtt
    25981 tgctggaaca cgccaaccgt caggcgagta acctggccta tggtgaccaa cgccgtctgg
    26041 agattgcccg ctgcatggtg acgcagccgg agattttaat gctcgacgaa ccggcggcag
    26101 gtcttaaccc aaaagaaacc aaagagctgg atgagctgat tgccgaactg cgcaatcatc
    26161 acaacaccac tatcttgttg attgaacacg atatgaagct ggtgatgggg atttcagacc
    26221 gaatttatgt ggtcaatcag gggacgccac tggcaaacgg tacgccggag caaatccgta
    26281 ataacccgga cgtgatccgt gcctatttag gtgaggcata agatggaaaa agtcatgttg
    26341 tcctttgaca aagtcagcgc ccactacggc aaaatccagg cgctgcatga ggtgagcctg
    26401 catatcaatc agggcgagat tgtcaccctc attggcgcga acggggcggg gaaaaccacc
    26461 ttgctcggca cgttatgcgg cgatccgcgc gctaccagcg ggcgaattgt gtttgatgat
    26521 aaagacatta ccgactggca gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg
    26581 gaagggcgtc gcgtcttctc gcggatgacg gtggaagaga acctggcgat gggcggcttt
    26641 tttgccgaac gcgaccagtt ccaggagcgc ataaagtggg tgtatgaact gtttccacgt
    26701 ctgcatgagc gccgtgttca gcgggcaggc accatgtccg gcggtgagca acaaatgctg
    26761 gcgattggtc gcgcgctgat gagcaacccg cgtttgctgc tacttgatga gccatcgctc
    26821 ggtcttgcgc cgattatcat ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag
    26881 gggatgacta tcttcctcgt cgagcagaac gccaaccagg cgctaaagct ggcggatcgc
    26941 ggctacgtgc tggaaaacgg ccatgtggtg ctctccgata ccggcgacgc gctgctggcg
    27001 aacgaagcag tgagaagtgc gtatttaggc gggtaataac acgttgattg ataaggagtc
    27061 aaaagactcc tttgagacag gtgacaaatg taaaattgcc tgatgcgctg cgcttatcag
    27121 gcctactggg tgagttgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt
    27181 ttacgccgca tccggcatga aacgatgggt aatctgtaga gtttgattca gaccttctat
    27241 tttccgctta tccgtgcccc atctcccatt ttccctcacc cacgccgtca ccgccttgtc
    27301 atctttctga caccttacta tcttacaaat gtaacaaaaa agttattttt ctgtaattcg
    27361 agcatgtcat gttaccccgc gagcataaaa cgcgtgaatt cgcgcattcg gtacaacaag
    27421 agagataaac gatgaaaccg ttacattata cagcttcagc actggcgctc ggactggcgt
    27481 taatggggaa tgcacaggca gtgacgacca ttccgttctg gcattctatg gaaggggaac
    27541 tgggtaaaga ggtggattct ctggcccaac gttttaacgc cgaaaatccg gattacaaaa
    27601 ttgtaccgac ctataaaggc aactacgaac agaatttaag cgcggggatt gccgcatttc
    27661 gtaccggcaa cgctccggct attttgcagg tttatgaagt tggcaccgcc accatgatgg
    27721 catcgaaagc cattaaaccg gtatatgacg tgtttaaaga ggcggggatt caattcgatg
    27781 agtcgcagtt tgtgccgacg gtttccggtt actactccga cagcaaaacg ggccacttac
    27841 tctcccagcc attcaacagc tcgacccccg ttctctatta caacaaagac gccttcaaga
    27901 aagcaggatt agacccggaa caaccgccga aaacctggca ggatctggcg gactatgccg
    27961 cgaaactgaa agcctccggt atgaagtgcg gctacgccag cggctggcag ggctggatcc
    28021 aactggaaaa ctttagcgcc tggaacggtc tgccgtttgc cagcaaaaac aacggctttg
    28081 acggcacgga cgcggtgctg gagttcaaca agccggagca ggtgaaacat atcgccatgc
    28141 tcgaggagat gaacaagaag ggcgacttca gctacgtcgg tcgtaaggat gaatccaccg
    28201 agaagttcta taacggtgat tgcgcgatga ccaccgcctc ttccggttct cttgccaaca
    28261 ttcgcgagta cgccaaattt aattacggcg taggcatgat gccttacgac gccgatgcga
    28321 aagatgcgcc acaaaacgcc attatcggcg gagccagcct gtgggtgatg cagggtaaag
    28381 ataaagaaac gtataccggt gtggcgaagt tcctcgattt cctcgcgaag ccagaaaacg
    28441 ctgccgagtg gcatcagaaa accggttatc tgccaattac caaagcggcg tatgacctga
    28501 cccgtgagca gggcttttac gagaaaaacc caggtgcgga taccgcgacg cgtcagatgc
    28561 tgaataagcc gccgttgccg ttcaccaaag ggctgcgtct gggcaacatg ccgcagatcc
    28621 gcgtgattgt ggatgaagag ctggagagcg tgtggaccgg taagaagaca ccacagcagg
    28681 cactggatac cgccgttgag cgtggaaatc agttgctgcg ccgctttgag aaatcgacga
    28741 agtcttaatc agtgtaatgt cggatgcgtt tcgcttatct gacttggcat cgcgtgtagg
    28801 ccggataagc gaagcgcatc cggcacagtt caggaattaa ccgtaatgtc atcatcccgt
    28861 ccggtgttcc gctcgcgctg gctgccttat ctgctggtcg cgccgcagct catcatcacc
    28921 gttatctttt ttatctggcc tgcgggcgaa gcgttgtggt actcgctaca aagcgtcgat
    28981 ccgtttggtt tctccagcca gtttgtcggc ctggataact tcgtcgcgct gtttcatgac
    29041 agctactatc tcgactcctt ctggacgacg ataaaattca gcacctttgt caccgtcagc
    29101 ggtttgctgg tgtcgctgtt cttcgcggcg ctggtggagt acatcgtgcg cggcagccgt
    29161 ttctatcaaa ccttaatgct gctgccttac gccgtggctc ccgccgttgc cgccgtattg
    29221 tggatcttcc tgtttaaccc cggtcgcggg ctgatcaccc attttctcgc ggagttcggc
    29281 tacgactgga accacgcgca aaacagcggt caggcaatgt ttctggtggt gtttgcctca
    29341 gtatggaagc aaatcagcta caacttcctg ttcttctatg ccgcgctgca atccattccc
    29401 cgttcgttga tcgaagccgc agccatcgac ggtgcaggcc caattcgccg cttctttaag
    29461 attgcgctgc cgcttatcgc tccggtgagt ttcttcctgc tggtggtgaa cctggtgtat
    29521 gccttcttcg acaccttccc ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc
    29581 accacgacgc tgatttataa gatctaccgt gaaggcttta ccggactgga tctggcttcg
    29641 tctgccgcgc agtcggtggt gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc
    29701 cgctatgttg aaagcaaggt gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat
    29761 tcagccatac catgctgatc ctcgggatcg cggtgatcct cttcccgctg tacgtggcgt
    29821 ttgtcgcggc gacgctggat aaacaggccg tctatgccgc gccgatgacg ctcatccccg
    29881 gcacacatct gctggaaaat atccacaaca tctgggttaa cggggtaggc acgaatagcg
    29941 cgccgttctg gcggatgttg cttaacagct ttgtgatggc gttcagcatt acgctcggca
    30001 aaattaccgt ctcgatgctc tcggcatttg ccattgtctg gtttcgtttt ccgctacgta
    30061 acctcttctt ctggatgatt tttatcaccc tgatgctgcc ggttgaagta cgtatcttcc
    30121 cgacggtgga agtcatcgcc aacctgaaga tgctcgacag ctacgccggt ttaacgctgc
    30181 cgctgatggc ctcggcgacc gccacgtttc tgtttcgcca gttctttatg acgctgccgg
    30241 atgaactggt ggaagcggcg cggatcgacg gcgcgtcacc aatgcgtttc ttttgcgaca
    30301 tcgtttttcc gctctccaaa accaatctgg cggcgctgtt tgtgatcacc tttatttacg
    30361 gctggaacca gtatttgtgg ccgttgttga ttattaccga tgtggatctc ggcaccaccg
    30421 tggcagggat caaagggatg atcgctacag gcgaaggcac cactgaatgg aactcagtga
    30481 tggcggcgat gttgttaacg cttatccctc cggtggtgat tgttttagtg atgcagcgtg
    30541 ccttcgtgcg cggcctggtc gatagtgaga aataagatgg caggactgaa attacaggca
    30601 gtaaccaaaa gctgggatgg taaaacccag gtcattaaac cgctgaccct tgatgtggcg
    30661 gatggcgaat ttatcgtgat ggtcgggccg tctggctgcg ggaaatcgac gctgctgcgc
    30721 atggttgccg ggctggagcg ggtgacagaa ggcgatatct ggatcaacga ccagcgcgtg
    30781 actgaaatgg agccaaaaga tcgcgggatt gcgatggtgt tccagaacta cgcgctttat
    30841 ccgcatatga gtgtcgaaga aaacatggcg tgggggctga aaattcgcgg catgggcaag
    30901 cagcaaattg ccgagcgcgt taaagaagcg gcgcgcattc tggagctgga cggtctgctc
    30961 aaacgtcgcc cgcgcgagct ttccggcggt cagcgccagc gtgtggcgat gggccgcgcg
    31021 attgtgcgcg atccggcggt gttcctgttt gatgagccgc tctctaacct cgatgccaag
    31081 ctgcgcgtgc agatgcgtct tgaactgcaa cagttgcacc gtcgcctgaa aacgacttca
    31141 ctctacgtta ctcacgatca ggttgaagcg atgacgctcg cccagcgagt aatggtgatg
    31201 aacggcggcg ttgccgaaca gattggcaca ccagttgaag tctacgaaaa gcccgccagc
    31261 ctgtttgtag cgagttttat cggcagtccg gcgatgaacc tgctgacagg ccgcgtgaat
    31321 aacgaaggca cgcatttcga actggacggc ggtattgagc tgccgctaaa cggtggctac
    31381 cgtcagtatg ccgggcgtaa aatgactctc ggcattcgcc cggaacatat tgcgctaagc
    31441 tcgcaggcag aaggcggcgt accgctggtg atggacacgc tggagatcct cggcgcagat
    31501 aacctggcgc acggacgctg gggcgagcag aagctggtgg tgcgactggc gcatcaggag
    31561 cgcccgacgg caggcagcac gctgtggctg catctggcgg aaaatcagct gcatcttttt
    31621 gatggtgaaa caggacaacg agtatgagta actggcctta tccccgcatc gtcgctcatc
    31681 gtggcggcgg taagctggcc ccggaaaaca ccctggcggc aatcgacgtc ggggcaaaat
    31741 acggtcataa gatgatcgaa tttgacgcga agttatcgaa agatggcgag atcttcctgc
    31801 tccatgacga caatctcgaa cgcaccagca acggctgggg cgtcgcgggt gaactgaact
    31861 ggcaggattt actgcgcgtg gatgcgggca gttggtacag caaagcgttt aaaggtgagc
    31921 cgctgccgtt gctttcgcag gtggcggaac gctgtcgcga acacgggatg atggcgaata
    31981 tcgaaatcaa acccaccact ggcactggac cattaacggg caaaatggtg gcgctggcgg
    32041 cacgccaact gtgggccggt atgacgccgc cgctgctgtc atcgtttgag attgatgctt
    32101 tagaagctgc acagctggcg gcaccggaac tgccgcgtgg tttattgctt gatgagtggc
    32161 gcgacgactg gcgcgaactg accgcgcggc tgggctgcgt ctctattcat ctcaatcata
    32221 agttactcga taaagtgcga gtgatgcagt tgaaagacgc cggactgtgg attctggttt
    32281 ataccgtcaa taaaccccag cgcgcggcag agttgctgcg ctggggcgtg gattgcatct
    32341 gtaccgatgc gattgacgtg attggtccga actttacggc ccaatagttt tcaacggaat
    32401 gtcaggctgc ggcgtgctgg gctgattcag catgtcgccg tttctcctct ccggcagcat
    32461 atgctgctga ttcaacgaac tatccggatt acggttgctg tttaacatcc cgccgttggt
    32521 gttgggcagc atttgctgcc gggcgggatt tcgctccccc ggctgtgact gcaacacccg
    32581 ctgagaatta ttgtttatct ggttttctaa atgctgctgt tgcaactgag tttgcgtttt
    32641 cagttgctga ttcagcatcc ctttttgctg gatttgctga gtctgcatct gggtttgcat
    32701 ccgctgctgg ctgggtatct gataccccgg ctggttaggg ttgttcagag tattaatggg
    32761 ctgtgcaaag ccgacaaacg gcaggagtgc cgtaagaatc agaagccgtt tcatcgcgta
    32821 tcctcctctg aagatatcct ttaagtttac tcgcttcccg acaaaacgat gattaattca
    32881 gagttatata ccaggcttag ctggggttgc cccttaatct ctggagaata acgatgataa
    32941 aaccgacgtt tttacgccgg gtggccattg ctgctctgct ctcaggaagt tgttttagcg
    33001 ccgccgccgc gcctcctgcg ccgcccgtct cgtatggtgt ggaggaagat gtcttccacc
    33061 cggtacgcgc gaaacaggga atggtagcgt ctgtggacgc cactgccact caggtggggg
    33121 tggatattct caaggagggc gggaatgccg ttgatgccgc cgtggcggtg ggctacgcgc
    33181 tggcggtaac gcatccgcag gcagggaatc tgggcggtgg tggttttatg ctgatccgct
    33241 cgaaaaatgg caataccacc gctatcgatt tccgcgaaat ggcacccgct aaagcgaccc
    33301 gcgatatgtt ccttgatgac cagggcaacc cggacagcaa aaaatcactc acttcgcatc
    33361 tggcttccgg cacaccaggt acggtcgctg gtttctcgct ggcgttggat aagtacggca
    33421 ctatgccgtt gaacaaagtg gtgcagcccg cgttcaaact ggcacgcgat ggttttatcg
    33481 ttaacgatgc tttggcagac gatctcaaaa cctacggtag cgaagtgttg ccgaatcacg
    33541 aaaacagcaa agctatcttc tggaaagagg gcgaaccgct gaaaaagggc gacacgctgg
    33601 tgcaggcgaa cctggcaaag agcctggaga tgattgctga aaacggcccg gacgaattct
    33661 ataaaggcac gattgcggaa cagatcgccc aggagatgca gaaaaacggt ggcttgatca
    33721 ctaaagaaga tttagcggcc tataaagcgg tcgaacgcac tccgataagc ggcgattatc
    33781 gcgggtatca ggtttactcc atgccaccgc catcctccgg cgggatccat atcgtacaaa
    33841 tcctcaatat tctggaaaac ttcgatatga agaaatacgg ctttggcagc gccgatgcga
    33901 tgcaaatcat ggcagaagcg gagaaatacg cctacgccga ccgctcggaa tatcttggcg
    33961 acccggattt tgtcaaagtc ccgtggcagg cactgaccaa taaagcctat gccaaatcca
    34021 ttgccgatca aattgatatc aacaaagcga agccgtccag tgagattcgc cctggcaagc
    34081 ttgcgcctta tgagagtaat caaactaccc attactctgt agtggataaa gacggtaacg
    34141 cggtggcggt gacctatacg ctgaacacca ccttcggtac gggtattgtc gcgggcgaga
    34201 gcggtattct gcttaataac cagatggatg atttctccgc caaaccgggc gtaccgaacg
    34261 tttacgggct ggtgggcggt gatgccaacg ccgtcgggcc gaacaaacgc ccgctgtcgt
    34321 cgatgtcgcc gaccattgtg gtgaaagacg gtaaaacctg gctggttacc ggtagcccag
    34381 gcggtagccg gatcatcact acagtgctgc aaatggtggt gaatagcatc gattatggca
    34441 tgaacgtcgc cgaagcgacc aatgcgccgc gtttccacca tcagtggttg ccggacgagc
    34501 tgcgtgtcga aaaagggttt agcccggata cgctcaagct gctggaagca aaaggtcaga
    34561 aagtggcgct gaaagaggcg atgggcagta cacaaagcat tatggttggg ccggacggtg
    34621 agttgtacgg cgcatccgac ccgcgctcgg tggatgattt aacggcgggg tactaaggtt
    34681 agcggccctc ttcgtgggaa gagggctatt ttgtcagggc aagccgaagg tagccttttt
    34741 tatttcgtaa tcctgtagat attcttccag cggctttgcc gttcccagag aatgaatttc
    34801 accactgtct ttatcaataa taaaaggtgc gttaccagct aagcgcgcgg cctcatctcc
    34861 agtttcgaga aattctcgtg cttcgaaaca gaaataccag ccct
//
LOCUS       DAZFJF010000039        34608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFJF010000039 DAZFJF010000000
VERSION     DAZFJF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.039"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            256..408
                     /gene="mokC"
                     /locus_tag="ACHKAU_002083"
     CDS             256..408
                     /gene="mokC"
                     /locus_tag="ACHKAU_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO5531147.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(512..1642)
                     /gene="dnaJ"
                     /locus_tag="ACHKAU_002084"
     CDS             complement(512..1642)
                     /gene="dnaJ"
                     /locus_tag="ACHKAU_002084"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO5531148.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1731..3647)
                     /gene="dnaK"
                     /locus_tag="ACHKAU_002085"
     CDS             complement(1731..3647)
                     /gene="dnaK"
                     /locus_tag="ACHKAU_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO5531149.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4024..4428
                     /gene="yaaI"
                     /locus_tag="ACHKAU_002086"
     CDS             4024..4428
                     /gene="yaaI"
                     /locus_tag="ACHKAU_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO5531150.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4454..5167
                     /gene="msyB"
                     /locus_tag="ACHKAU_002087"
     CDS             4454..5167
                     /gene="msyB"
                     /locus_tag="ACHKAU_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5531151.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5316..5882
                     /gene="satP"
                     /locus_tag="ACHKAU_002088"
     CDS             5316..5882
                     /gene="satP"
                     /locus_tag="ACHKAU_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO5531152.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5917..6504)
                     /gene="mog"
                     /locus_tag="ACHKAU_002089"
     CDS             complement(5917..6504)
                     /gene="mog"
                     /locus_tag="ACHKAU_002089"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO5531153.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6619..7572)
                     /gene="tal"
                     /locus_tag="ACHKAU_002090"
     CDS             complement(6619..7572)
                     /gene="tal"
                     /locus_tag="ACHKAU_002090"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5531154.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7851..9281
                     /gene="yaaJ"
                     /locus_tag="ACHKAU_002091"
     CDS             7851..9281
                     /gene="yaaJ"
                     /locus_tag="ACHKAU_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO5531155.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9351..10127
                     /gene="yaaA"
                     /locus_tag="ACHKAU_002092"
     CDS             9351..10127
                     /gene="yaaA"
                     /locus_tag="ACHKAU_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO5531156.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10280..10576)
                     /gene="yaaX"
                     /locus_tag="ACHKAU_002093"
     CDS             complement(10280..10576)
                     /gene="yaaX"
                     /locus_tag="ACHKAU_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO5531157.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10790..12076)
                     /gene="thrC"
                     /locus_tag="ACHKAU_002094"
     CDS             complement(10790..12076)
                     /gene="thrC"
                     /locus_tag="ACHKAU_002094"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO5531158.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12077..13009)
                     /gene="thrB"
                     /locus_tag="ACHKAU_002095"
     CDS             complement(12077..13009)
                     /gene="thrB"
                     /locus_tag="ACHKAU_002095"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO5531159.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPLGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13011..15473)
                     /gene="thrA"
                     /locus_tag="ACHKAU_002096"
     CDS             complement(13011..15473)
                     /gene="thrA"
                     /locus_tag="ACHKAU_002096"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO5531160.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15500..15619)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15555..15620)
                     /gene="thrL"
                     /locus_tag="ACHKAU_002097"
     CDS             complement(15555..15620)
                     /gene="thrL"
                     /locus_tag="ACHKAU_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO5531161.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(15834..16520)
                     /gene="yjtD"
                     /locus_tag="ACHKAU_002098"
     CDS             complement(15834..16520)
                     /gene="yjtD"
                     /locus_tag="ACHKAU_002098"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5531162.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16920..17060)
                     /gene="yjjY"
                     /locus_tag="ACHKAU_002099"
     CDS             complement(16920..17060)
                     /gene="yjjY"
                     /locus_tag="ACHKAU_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO5531163.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17156..17872
                     /gene="arcA"
                     /locus_tag="ACHKAU_002100"
     CDS             17156..17872
                     /gene="arcA"
                     /locus_tag="ACHKAU_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO5531164.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17932..19284)
                     /gene="creD"
                     /locus_tag="ACHKAU_002101"
     CDS             complement(17932..19284)
                     /gene="creD"
                     /locus_tag="ACHKAU_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO5531165.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(19342..20766)
                     /gene="creC"
                     /locus_tag="ACHKAU_002102"
     CDS             complement(19342..20766)
                     /gene="creC"
                     /locus_tag="ACHKAU_002102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO5531166.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20766..21455)
                     /gene="creB"
                     /locus_tag="ACHKAU_002103"
     CDS             complement(20766..21455)
                     /gene="creB"
                     /locus_tag="ACHKAU_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO5531167.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21468..21941)
                     /gene="creA"
                     /locus_tag="ACHKAU_002104"
     CDS             complement(21468..21941)
                     /gene="creA"
                     /locus_tag="ACHKAU_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO5531168.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22152..23021
                     /gene="robA"
                     /locus_tag="ACHKAU_002105"
     CDS             22152..23021
                     /gene="robA"
                     /locus_tag="ACHKAU_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO5531169.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23018..23665)
                     /gene="gpmB"
                     /locus_tag="ACHKAU_002106"
     CDS             complement(23018..23665)
                     /gene="gpmB"
                     /locus_tag="ACHKAU_002106"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO5531170.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23717..24238
                     /gene="yjjX"
                     /locus_tag="ACHKAU_002107"
     CDS             23717..24238
                     /gene="yjjX"
                     /locus_tag="ACHKAU_002107"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO5531171.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24323..24649)
                     /gene="trpR"
                     /locus_tag="ACHKAU_002108"
     CDS             complement(24323..24649)
                     /gene="trpR"
                     /locus_tag="ACHKAU_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO5531172.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24739..26676)
                     /gene="sltY"
                     /locus_tag="ACHKAU_002109"
     CDS             complement(24739..26676)
                     /gene="sltY"
                     /locus_tag="ACHKAU_002109"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO5531173.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            26887..28554
                     /gene="ettA"
                     /locus_tag="ACHKAU_002110"
     CDS             26887..28554
                     /gene="ettA"
                     /locus_tag="ACHKAU_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO5531174.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28861..30093)
                     /gene="nadR"
                     /locus_tag="ACHKAU_002111"
     CDS             complement(28861..30093)
                     /gene="nadR"
                     /locus_tag="ACHKAU_002111"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO5531175.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30114..31496)
                     /gene="radA"
                     /locus_tag="ACHKAU_002112"
     CDS             complement(30114..31496)
                     /gene="radA"
                     /locus_tag="ACHKAU_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO5531176.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31545..32513)
                     /gene="serB"
                     /locus_tag="ACHKAU_002113"
     CDS             complement(31545..32513)
                     /gene="serB"
                     /locus_tag="ACHKAU_002113"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO5531177.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            32619..33263
                     /gene="ytjB"
                     /locus_tag="ACHKAU_002114"
     CDS             32619..33263
                     /gene="ytjB"
                     /locus_tag="ACHKAU_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO5531178.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33291..34307
                     /gene="lplA"
                     /locus_tag="ACHKAU_002115"
     CDS             33291..34307
                     /gene="lplA"
                     /locus_tag="ACHKAU_002115"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO5531179.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34339..34602
                     /locus_tag="ACHKAU_002116"
     CDS             34339..34602
                     /locus_tag="ACHKAU_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5531180.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT         8176 a         9421 c         8641 g         8370 t
ORIGIN      
        1 ttaaataatt aaatcattaa aaaattacgc cacaaaaatg cgaggcgtct tcaggattag
       61 aatcgataaa acagaatggg ttaaagagag cgatataata gcggcgggta cttgaggcta
      121 tctgtctcag gcattagctg aacggcagat agagaaaagc cccgagtgat attttactat
      181 caacccgagg ccccctatat gctgaacaca tgtagagtgc ctcttactga ccgtaaggtc
      241 aaggagaaga gagcaatgaa gcagcataag gcgatgattg tcgccctgat cgtcatctgt
      301 atcaccgccg tagtggcggc gctggtaacg agaaaagacc tctgtgaggt tcacatccga
      361 actggccaga cggaggttgc tgttttcacg gcttacgaat ccgagtaagt gcaacggcgg
      421 ggagtgatcc ccgccacttt tcaggtgctc gcatatcttc aacgcaccct aattttaccc
      481 aagcctgccc acgggcaggc ttttggggag gttagcgggt caggtcgtca aaaaacttct
      541 tcacaccatc aaagaagctc tttgagcgcg ggctgttgtg ctcaccggtt gggccaccga
      601 agctttcttg cagctcttgc agcagctgtt tctgcttctc gttcaggcct accggtgttt
      661 cgacgacaac gcggcacagc aaatcaccct gtgcgccacc gcggacagac ttgacgcctt
      721 taccgcgcat acggaaaagc ttaccggtct gggtttcgcc aggcactttc agtttgacgc
      781 gaccatcaag ggtcggtact tcgatttcgc cacccagcgc cgccatagcg aagttgatcg
      841 ggacttcgca atagaggttg ttgccttcac gctcgaaaat cgggtgctgt ttaacctgaa
      901 cctgaacgta cagatcgcct gccggtgcgc catgctcgcc cgcttcacct tcgcccgcaa
      961 gacggatgcg gtctccagtg tccacccctg ccgggatttt aacggacagc gttttgctgc
     1021 gctcaacacg accatgacca tgacatttgt tgcacggatc tttgatcagc gtaccgcggc
     1081 cctgacagtg tggacaggtc tgctgtacag cgaagaatcc ctgacgcatc tgcacctgac
     1141 cagaaccatg acaggtcgga caggtctgcg gctgtgtacc tggttttgca ccgctaccgt
     1201 ggcaaacgtc acactcttcc agagtcggaa tgcggatctc tttggtcacg ccacgtacag
     1261 cttcttcgag ggtgagctcc atgttatagc gtaaatcagc accgcgcgcc gcacgttgac
     1321 gaccacgtcc gccgccaaaa atatcgccga aaacgtcacc aaaaatatcg ctgaagtctg
     1381 cgccgccgcc aaaaccgccg ccgcccatac caccttgctc aaacgcagca tgaccatact
     1441 gatcgtatgc cgcacgtttt tgcgagtcgg tcagaacttc ataagcttcc ttgatctctt
     1501 taaatttcgc ctcggcctct ttgtcaccct ggttacggtc cgggtggtat ttcatggcca
     1561 ggcgtttgta ggcctttttg atttcacgct cttccgctgt tttggaaacg cctaaaatct
     1621 cgtaataatc ttgcttagcc atctttttta aattgcccct agatgaatgc acgggcggag
     1681 aggaaattcc ccttcgcccg tgtcagtata attacccgtt tatagggcga ttattttttg
     1741 tctttgactt cttcaaattc agcgtcgaca acatcgtcat ctttcgcgtt gtttgcagaa
     1801 gcatcagcac cggcagtctg ctgctgggca tgttgctgct gggcgatttc catcagtttc
     1861 tgggaaacct gtgccagctc ctgcattttc gcttcgatag cggctttgtc ttcacctttc
     1921 agagcagttt ccagtgcagt cagtgcagac tcgatagcag ttttgtcgtc agccggcagt
     1981 ttgtcgcctg cttcttcaac ctgcttacgg gtgctgtgca gcagatggtc gccctggttg
     2041 cgagtctgta ccagctcttc aaacttacgg tcagcttcgg cgttagcttc tgcgtcgcgt
     2101 accattttct ggatttcatc ttcgttcaga ccagaagaag ccttgatggt gatcttctgc
     2161 tctttaccgc tgtttttatc tttcgcggaa acgtgcagga taccgtcagc atcgatatcg
     2221 aaggtaactt cgatctgcgg catgccgcgc ggtgccgggt tgataccatc caggttgaac
     2281 tgacccagag atttgttatc agccgcacgt ttacgttcac cctgcagcac atggatggtt
     2341 accgcagact ggttgtcttc agcggtagag aacacctggc tgtgcttggt cgggatagtg
     2401 gtgtttttcg cgatcagcgt cgtcatcaca ccgcccatgg tttcgatacc cagagacagc
     2461 ggggtaacgt ccagcagcag tacgtctttc acgtcaccag tcagaacacc accctgaaca
     2521 gcagcaccga ttgctacagc ttcgtccggg ttaacgtctt tacgcggctc tttaccaaag
     2581 aactcagcaa ctttcttctg aaccattggc atacgagtct gaccaccaac gaggataacg
     2641 tcgtcgatat cagatacgga caggccagcg tcctgcagtg caactttcag cggctcaatg
     2701 gaacggttta ccagatcttc aaccaggctt tccagtttcg cacgagtcac tttgatgttc
     2761 atgtgtttcg gaccggtcgc atctgcagtg atgtacggca ggttaacgtc ggtctgctga
     2821 gcggaagaca gttcgatttt cgctttttct gccgcttctt tcaggcgctg cattgccagc
     2881 ggatcgttgc gcaggtcaat gccctgatct ttcttgaatt cttcaaccag gtagttgatc
     2941 agacggctgt cgaagtcttc accacccagg tgggtatcac cgttggtagc cagaacttcg
     3001 aaggtttttt cgccgtcaac ttcgtcgatt tcgataatag aaatatcgaa agtaccaccc
     3061 cccaggtcat aaaccgcgat agtacggttg ccagtacctt tgtccagacc gtaagccagc
     3121 gcagctgcgg tcggttcgtt gatgatacgt tttacttcca gaccagcgat acggcctgcg
     3181 tctttggttg cctgacgctg agcatcgtta aagtatgccg gtacggtgat aacagcttca
     3241 gttaccggtt cacccaagta atcttcagcg gttttcttca tttttttcag cacttcagca
     3301 gaaatctgcg gcggtgccat tttctggcct ttaacttcga cccatgcgtc gccgttatca
     3361 gcagcaataa ttttgaacgg catgatggaa acatcacgct gtacttcttc gtcctggaag
     3421 cggcggccaa tcaggcgttt aatcgcaaac agggtgtttt gcgggttcgt cactgcctga
     3481 cgtttagccg gctgaccaac cagagtttca ccatcctggg tataggcaat aatagaaggc
     3541 gtggtgcgat cgccttcggc gttctccagc acgcgaggag tggtgccatc cataatcgct
     3601 acacaagagt tggtagtacc caggtcgata ccaattattt tacccatcta aacgtctcca
     3661 ctatatattc ggtcatcatg tggttgtgag tctgtaatag gggcgaaacg tctggtttca
     3721 actgcccaat ttcatttttt tgcagactca ctcactgcgg ttgactacta aatggggtcg
     3781 taacccacgt catcaagggg gagatgcaaa aattttttgt gcttttgcgc agaaatttcg
     3841 cgatatagca ggacaagacg tgaaactgac agcaatttta ttcatataaa gaatgaatcg
     3901 cagccaagac attcatcaaa aaattgtaat aaaaagaaaa cattacgcac ctgaatcttc
     3961 tctttatcag cagtaaacta gtgggtatcc atccccctac ctctccccac taagagaatc
     4021 cttatgaaat ccgtttttac gatttccgcc agcctggcga ttagtctgat gctgtgctgc
     4081 acggcgcagg caaacgacca taaaatcctc ggcgtcattg cgatgccacg taacgaaacc
     4141 aacgatctgg cgctgaaact tcctgtttgc cgaattgtta aacgcataca actctctgcc
     4201 gaccatggcg atttacagtt aagcggtgca tcggtttatt tcaaagccgc ccgtagcgcc
     4261 agtcagagcc tgaatattcc ttcagaaata aaagaagggc aaaccactga ctggatcaac
     4321 attaacagcg ataacgacaa taaacgctgc gtctcaaaaa tcaccttttc cggtcatacg
     4381 gtgaactcat cggatatggc cacgctgaaa attatcggcg acgactaacg ccagaatata
     4441 gaagccacaa aaaatgaatg ttaattacct gaatgattca gatctggatt ttctccagca
     4501 ttgcagtgag gaacagttgg caaatttcgc ccgattgctc acccataatg aaaaaggcaa
     4561 aactcgcctc tccagcgtac tgatgcgcaa cgaactgttt aaatctatgg aagggcatcc
     4621 cgagcaacat cgccgcaact ggcagctgat tgctggagaa ttacagcatt ttggtggcga
     4681 tagtatcgcc aacaaactgc gcggacacgg taaattgtat cgggccattt tgctcgatgt
     4741 ttcaaaacga ttgaagctga aagccgacaa agagatgtct acgtttgaga ttgagcaaca
     4801 gttactggaa caatttctgc gtaacacctg gaagaatatg gacgaggaac ataaacagga
     4861 gtttctgcac gcggtcgatg cgagggtgaa tgagctggaa gagctgctgc cgctgctgat
     4921 gaaagacaaa ttattggcaa aaggcgtgtc gcatttgctt tccagccaac tgacccgcat
     4981 tttacgcacc cacgcagcaa tgagcgtact tgggcatggt ttgctgcgcg gcgcggggct
     5041 gggaggccct gtaggtgcgg cactaaatgg ggttaaagcg gtcagcggca gcgcctatcg
     5101 cgtgacgatt ccagccgtac tgcaaatcgc ctgcctgcgc cggatggtta gcgccactca
     5161 ggtctgatac cggaaaccgg ggaaaatttt cattttcccc tcaaaagatc gtagacactg
     5221 ccccactggc tgattattat gccgcgccct gaaaacacta cagttattca gggaaattat
     5281 ttcaccattc attcgatgat gatttttgag gaattatggg caacactaag ttggctaatc
     5341 cggcaccgct gggcctgatg ggcttcggca tgaccaccat tctgcttaac ctgcacaacg
     5401 tgggttattt cgctctggac ggtattattc ttgccatggg cattttctac ggcggcatcg
     5461 cgcaaatttt tgccggtctg ctggagtaca aaaaaggcaa cactttcggt ttaaccgcat
     5521 tcacctctta cggttctttc tggctgacgt tggttgcgat tctgctgatg ccgaaactgg
     5581 gtctgaccga tgcgccaaat gcacagttcc ttggtgtcta cctgggtctg tggggcgtat
     5641 ttacgctgtt tatgttcttc ggcacgctga aaggcgcacg cgttctgcaa ttcgttttct
     5701 ttagcctgac tgtgctgttt gccctgctgg cgatcggtaa cattgccggt aacgccgcaa
     5761 tcatccactt tgccggctgg attggtctga tctgtggtgc cagtgcaatc tatctggcga
     5821 tgggtgaagt actgaacgag cagtttggtc gcaccgttct gccgattggt gaatcccact
     5881 aattgttttg agatcccccc gctcgggggg atttttttat tcgctaacgt cgcgtcttgc
     5941 actcttcggt ctgaatgcag caaccacttc tggtgccgtt tcaacgtatg gcccttccag
     6001 caactgaatg cagtacggta cgctggcaaa aataccgtgt accacaacgt tacccgcagc
     6061 gtccttcaca ccttccagcg tctctttaat agacttcggc tgaccgggta agttaaggat
     6121 cagcgcctgt ttgcgaatca caccaacctg acgcgaaagg atcgcagttg gtacaaaatg
     6181 caggcttatc tggcgcatct gttcaccaaa gccaggcatc tcgcggtccg ctactgccag
     6241 cgtcgcatcg ggcgttacgt cacgacgcgc cgggccagtt ccgcccgtgg tgagcaccag
     6301 atggcaactc atttcatcca ctagctcaca caacgtttgc tcgatgatcg cctgctcatc
     6361 ggggattaag cgggtttcca gttcaaacgg cgtggttagc gccgatgtca gccattcttc
     6421 cagcgcaggg atgcctttat cctgataaac gccgctggat gcgcgatcag agatggaaac
     6481 taagccaata cgtaaagtat tcatcaagat ttttccgttt aaacagtctc gttaaacaga
     6541 atgatacact gcgaagggag tgacagacag gcttcagaag aggtagcgtg accgacttcc
     6601 cggtcacgct aagaatgatt acagcagatc gccgatcatt ttttccagtt tttcctggtc
     6661 aatagcaaac ttacggatac cttccgccag tttatctact gccattggat cctggttgtg
     6721 ctgccacagg aactcggact cagtgatacg cgccggacgc gctttcactt cgccggtgta
     6781 agacagttta cgttcgatag ccccttcgct ctcagccagc tctttcagca gtgtaggtgc
     6841 gatggtaaga cggtcgcagc ctgccagttc cagaatttcg ccgatgttac ggaagcttgc
     6901 gcccataacc acggtttcat aaccgtgctc tttgtagtac tggtagattt cagatacaga
     6961 aaccacgccc ggatcttctg ccggagcgta ctctttctta tcggtattcg ctttgtacca
     7021 gtcaagaata cggccaacaa acggcgagat caggaacacg cccgcttccg cacaagcacg
     7081 agcctgagcg aaggagaaca gcagggtcag gttacagttg atgccttctt tttccagctg
     7141 ttctgcagca cggataccct gccaggtaga agccagtttg atcagaatac gatcgttgct
     7201 aataccagca tcgttgtaga gtttgatcag gcgttttgct ttcgcaattg acgcttcggt
     7261 gtcataggaa agacgcgcat caacttcagt tgagatacgg cccggaacca gtttcaggat
     7321 ttccagacca atatttactg ccagtttgtc ggtcgcgtcc acgatctgct gcgcgcgatc
     7381 gttgctctgc tgtttcgccc aggcgacagc atcatcaatc aacttacggt attccggaat
     7441 ctgcgctgcg ttaagaatga gagaagggtt ggttgtggca tcctgcggtt gatacagctt
     7501 cattgccgcg atgtccccag tgtcggccac tacggtggtg tactgacgaa gggaggtcaa
     7561 tttgtccgtc atgatagtat ttctctttaa acagcttgtt agggggatgt aaccggtctg
     7621 ccctgatgat atcacgacgc aacgtcagcg caaccgccga caagacggtt atcgccaggc
     7681 gagactgttt cggatttctg acactgcgtt gatatcgccc gccattttta tacaaaacct
     7741 catgtatgct acgcagaagt tatcaagtac ctcgtagcgt atatacttct taaacaattg
     7801 gtaacgttta cacaggaaag tcatcgcgac cggcaataag agggatatgc atgccagatt
     7861 ttttctcctt tattaacagc gtcctttggg gatcggtaat gatttacctg ctcttcggcg
     7921 caggttgttg gttcactttt cgcaccggat ttgtgcagtt tcgctacatc cgccagtttg
     7981 gcaaaagtct taaaaatagc attcatccac agccaggcgg tttaacctca tttcagtcat
     8041 tgtgtaccag tcttgcggcg cgcgtgggta gcggcaacct ggccggcgtt gcgctggcaa
     8101 ttaccgccgg tggacctgga gccgtcttct ggatgtgggt tgccgcgttt atcggcatgg
     8161 cgacctcgtt tgccgaatgt tccctcgcac agctttataa agaacgtgat gccaatgggc
     8221 agtttcgtgg cggaccggca tggtatatgg cgcgcgggct ggggatgcgc tggatgggcg
     8281 ttctgttcgc cgtctttttg ctcatcgcct acggcataat tttcagcgga attcaggcga
     8341 acgccgttgc gcgagccctg agtttttctt ttgattttcc cccgctggtg acaggtatta
     8401 ttctcgctgt ctttgctctg ctggcgatca ctcgcggtct tcatggcgtc gcccggctca
     8461 tgcaggggtt tgtcccgttg atggcgataa tctgggtact gaccagcctg gtgatttgcg
     8521 taatgaatat cgggcaactt ccccacgtca tttggtctat ttttgagagt gcttttggct
     8581 ggcaggaagc ggcaggcggc gcggcgggat ataccttaag ccaggcgatt actaacggtt
     8641 ttcagcgcag tatgttttcc aatgaggcgg gaatggggtc gacgccaaac gcggcagcgg
     8701 cagcggcgtc ctggcctccg catccggcag cgcaagggat tgtccagatg attggcattt
     8761 ttatcgacac cctggtcatc tgtacggcaa gcgccatgct gatattactg gcgggtaacg
     8821 gcacaaccta catgccgctg gaaggtattc agcttatcca gaaggcgatg cgggtgctaa
     8881 tgggttcctg gggtgctgag tttgttaccc tcgtggttat tctgtttgcc ttcagctcca
     8941 tcgttgccaa ctacatttac gccgaaaaca atctcttctt tttacgcctg aacaacccta
     9001 aagcgatctg gtgtttgcgg atctgcacct tcgcaacggt catcggcggc accttgctaa
     9061 gtcttccgct gatgtggcaa ctggcagata tcataatggc ctgcatggct attaccaatt
     9121 tgaccgccat tttactgctc tcgcctgtgg ttcataccat tgccagtgat tatctacgcc
     9181 agcgtaaact cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat atcggccgcc
     9241 agctttctcc ggacgcgtgg gatgatgttt cgcaggagta atcacaacta tcgatcaact
     9301 cattctcttt ttttgctaaa gtcggcataa atttcctgca aggactggat atgctgattc
     9361 ttatttcacc tgcgaaaacg cttgattacc aaagcccgtt gaccaccacg cgctatacgc
     9421 tgccggagct gttagacaat tcccagcagt tgatccatga agcgcggaaa ctgacgcctc
     9481 cgcagattag cacgctgatg cgcatcagcg acaaactggc gggtatcaac gccgctcgct
     9541 ttcatgactg gcagccagat ttcacgccgg agaatgcccg ccaggcgatt ctggcgttta
     9601 aaggtgatgt ctacaccggc ttgcaggccg aaaccttcag cgaagacgat ttcgattttg
     9661 cccaacagca tttgcgaatg ctttccggct tgtatggcgt actgcgcccg ctcgatttaa
     9721 tgcagcctta tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg aaagatctgt
     9781 atcaattctg gggagatatc atcaccaaca agctgaacga ggcgctcgca gcacaaggcg
     9841 ataatgtggt gattaatctg gcgtcagatg agtattttaa atcggtgaag ccgaagaaat
     9901 tgaatgccga gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa tttaagataa
     9961 tcagcttcta cgctaagaaa gcacgcggtc tgatgagtcg ttttattatt gaaaatcggc
    10021 tgaccaaacc agagcaactg actggtttta atagcgaagg ttacttcttt gatgaagctt
    10081 cctccagcaa tggcgaactg gtgtttaaac gctacgagca gcgttaagtt tgcagtcaat
    10141 gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc atgcagattc aatatattgc
    10201 agaattcacg taggcctgat aagacgcgcc agcgtcgcat caggcgctga atgccggatt
    10261 gttacccggc atttgtcatt tagcggtgat gtttgcctgg accatgaccg ccgtgatgat
    10321 catgatgagc tttcttatgg tgacgcggcg gtggcggcgg tccgtgtggg tgccagcgat
    10381 tgcctcgcca ttcataatgt tgtttccacc agccgtggtc gcgccagtga ccgccatccc
    10441 agtaataacc gcgattatca cgatcgccta tctgtaattt tactgacggg actaacgtaa
    10501 tttccgcagc ctgcgctgcc atgggagcga ccagaaccag ggaaagtgcg agtacgatag
    10561 attgcatctt tttcacaggt tactcctttt atcattgccc gttatgggtc gatatccgga
    10621 gattacgcga tgagaacggc ggttgttatt ctccgcaatc ctaatctcta agttaccgca
    10681 tttattgaga atttctcctt tttccctgtc gtttctctcc atattttctt cacaaaaaag
    10741 ccgggctgca taaaagcaaa cccggcctga ttgagataat gaatagatgt tactgatgat
    10801 tcatcatcaa tttacgcaac gcagcaaaat cggcgggcag attatgtgaa agcaagggta
    10861 aatcagcacg ttctgccagc tcttttggca gatccaacgt ttcaccgaga atcgcttcca
    10921 cgctctcttt aaatttcgcc ggatgcgcgg tgccgaggaa caagccatat tcgcctggat
    10981 tcaactggtc acgcagcgca cgatacgcta cggcagcgtg cggctccgag gtgtagccca
    11041 gttcttttaa ctcacgcatt gtctgttgcg tggtttcatc atccacggct gcataaccca
    11101 gctctttcag ttgccagatt ttgcggcgga acaactcttc cacacgcggc cagttgttcg
    11161 gctgactcac gtccatcgcg ttggataacg tcgcctgagt cgctttgggg gaccactgac
    11221 cgtcgtgcag gaaacgtggc acggtatcgt tcacgttggt cgcagcaata aaacgtttca
    11281 ccggcagacc gagtgacttc gccagcagac ccgccgtcaa atcgccgaag tttccgcttg
    11341 gtaccgaaac aaccagctgg ttgcgcgcct cctgcggcag ctgcgcaaca gcttcaaagt
    11401 agtagcaaat ctgcgccagc aaacggctga tgttaatcga gttagctgag tttaacccca
    11461 gcgccacttt cagttcttca tcatcaaacg cctgcttcac cagcgcctga caggcatcga
    11521 aatcgccgtc gatggcaaca gtttcgatat tgccgcccaa tgtacagaac agtttttctt
    11581 gcagtggact gattttgcct cgtggataga ggataaccac tttcacattc ggcaaaccgt
    11641 agaaagcatg agccactgcc gctccggtat caccggaggt cgcggtcaga atggtcactg
    11701 gcttatcacc cgcaatatgg gtcagcattt gtgccataaa gcgaccgccg aaatctttaa
    11761 atgccagcgt tggcccgtgg aacaattcca gacaaccgac atcgctttca acattggcga
    11821 ccggagccgg gaaggcaaac gccgcgcgca cgcgctcttc caggatttcc tgcgggattt
    11881 catcaccaat aaacgccgag aggatcttcg cactgcgggt gacaaaatcc agcttcagca
    11941 tctcatcaat ttcagtcagg ctgaattccg gcaggtcatg cggaaaaaac agcccctgat
    12001 ttttgcccaa cccctgggtt acggcttgcg caaagctgac ctgctcgttg tgatctttca
    12061 gattgtagag tttcatttag ttttccagta ctcgtgcgcc cgccgtatcc agccggcaaa
    12121 tatgaacaaa accttcctga ttttgcaggt agttcttacc caaccagtcg gcaacgcgct
    12181 gggcggtatc cggcttgtca cacagagcga acaaggtcgg gccggagccg gagataccgc
    12241 tcgctaccgc gccgatttcc gcgaccgcct gccgcgcctg ccggaagcct ggcagtaatc
    12301 gttcacggta gggttcagcg ataacatctt tcatcagctt cgcggcaagc tcaggctgac
    12361 gggaatagca ggcgtgaatg aagcctgcca gatgtcgccc gtgcgcaatg caatcctggc
    12421 ggcgatactg cgccggtaaa atagcccggg cttctgccgt cgagacttta atccccggat
    12481 acgccagcac ccacagccac tcatcaaacc caggcacttg ctggctgatg atgtcgtttt
    12541 cttcgatcat caactgcata ccacctagaa aacacggtgc cacgttgtcg taatgaatgc
    12601 tgccggagat acgtccttcc agctcgccca tcaaagccag caaacgagtg tcattaagcg
    12661 gcttgccgca gtgttcattc atcgccatca gcgccgcgac caccgaacag gcgctggagc
    12721 ctaagcccga accgagcggc atattctttt ccagagtcat cgccactgga atttgcttgc
    12781 caagctcctg gcaaaaacgc tcccagcact gataaacgat attttcccgt ggttctgacg
    12841 gcagcttatc ggcaaagcgc ccgaggttgt tgagactgaa tatctctgcc gcctcaaccg
    12901 taactacatc tccgagcaat gcaccatcaa cgggtgtcac cgccgccccg agcacatcaa
    12961 acccgacgct catattggca ctggaagccg gggcataaac tttaaccatg tcagactcct
    13021 aacttccatg agagggtacg cagcagatcg gcaaagacac cagcagcggt aacgtcattg
    13081 cccgcaccat atccgcgcag caccaacggc agcggctgat aatagtggct atagaaggcc
    13141 agggcgtttt cgccattttt cactttgaac agcggatcat taccatccac ttcggcaatc
    13201 ttcacgcgac agacgccatc ttcatcaata ttgccaacat agcgcaaaac ttttccttca
    13261 tcacgggcct tcgccacgcg cgcggcaaag cgatcgtcga gctgtgacag attcgccata
    13321 aaagcggcaa catcaccctc ggcgttaaac tctgcgggca gcacaggttc aatttcaata
    13381 tccgccagct ccagttcacg tcccgtttca cgggcgagaa tcaatagttt acgcgccaca
    13441 tccataccag aaagatcatc tcgcggatcc ggttcggtat aacccatttc ccgtgccagc
    13501 gtggtcgcct cggagaaact catgccttcg tctaacttgc cgaagatata agaaagcgat
    13561 cctgaaagaa tgccggagaa cttcatcaat tcatcaccag cattgagcag attttgcagg
    13621 ttttcaataa ccggtaatcc agccccaacg ttggtgtcat agaggaattt ccgccgcgat
    13681 ttttccgccg catgacgcaa cagatggtag taatccatcg acgaggtgtt ggcctttttg
    13741 ttcggcgtga caacgtggaa accttcgcgc aggaagtcgg catattgatc cgccactgcc
    13801 tggctggagg tgcagtcaac aatgaccggg ttcagcagat gatattcttt cacgaggcga
    13861 attaagcgcc cgagattaaa cggctctttg gcttgcgcca gttcttcctg ccagttttcc
    13921 agatttaggc catgcacatt ggtgagcagt gccttcgagt tggcaacacc gcagacacgt
    13981 aagtcgatat gtttattctt cagccagctt tgttgacgct tcagttgctc cagcagcgca
    14041 ccgccaacgc caccgacgcc aatcacaaac acttcgataa cctgatcggt attgaacagc
    14101 atctgatgag taacgcgcac gccagtggtc gcatcatcgt tatttaccac gacagagatt
    14161 gagcgttcag aagatccctg agcaatagcg acaatgttga tattggcgcg ggccagcgcg
    14221 gcaaagaatt tcgccgagat cccacgcaag gtacgcatac catcacctac caccgagata
    14281 atggccagcc gttccgtcac cgccagcggc tccagtaagc cttctttcag ttccaggtag
    14341 aactcttcct gcattgcccg ttcagcccgc acacagtcgc tttgcggaac gcagaaactg
    14401 atactgtatt cggaagatga ttgcgtaatc agcaccacgg aaatacgggc gcgtgacatc
    14461 gcggcaaaga cgcgcgccgc catgccgacc attcctttca tccccgggcc ggaaacgctg
    14521 aacattgcca tgttattcag attggaaatg cccttgaccg gtaattcgtc ttcatcacga
    14581 ctggcaccaa tgagcgtacc tggtgcttga ggatttccgg tatttttaat caggcaaggg
    14641 atctggaact gggcgatggg ggtaatggtg cgggggtgaa gaactttagc gccgaagtag
    14701 gaaagctcca tcgcttcctg gtaggacatc gacttcaaca acctcgcatc gggcacctga
    14761 cgcgggtcgc aggtatagac cccgtcaacg tccgtccaaa tctcgcaaca atcggcgcgt
    14821 aaacaggcag ccagcaccgc cgcggagtag tcggaaccgt tgcgtccaag taccaccagt
    14881 tcgccttttt cattaccggc ggtgaagcct gccatcagca ccatgtgatc agccggaata
    14941 cgacttgccg caatacggcg ggtggactca gcaatatcga cggtagattc gaggtaatgc
    15001 cccactgcca gtagtttttc gaccggatcg ataacggtaa cgttgtgacc acgcgcttct
    15061 aacacgccgg ccataatggc gatcgacatt ttctcgccac ggcaaatcag cgcagcgtta
    15121 atgctatccg ggcactgccc taacaaacta atgccatgca ggacatgttt tatttgggca
    15181 aattcctggt cgacgaaagt tttcaattgc gccagcggga atcccggctg ggcggcggcg
    15241 agtcccgtca aaagttcggc aaaaatacgt tcggcatcgc tgatattggg taaagcatcc
    15301 tggccgctaa tggttttttc aatcatcgcc accaggtggt tggtgatttt ggcgggggca
    15361 gagaggacgg tggccacctg cccctgcctg gcattgcttt ccagaatatc ggcaacccgc
    15421 agaaaacgtt ctgcatttgc cactgatgta ccgccgaact tcaacactcg catggttgtt
    15481 acctcgttac ctttggtcga aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt
    15541 gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtgataatg
    15601 gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tctgtgcgct
    15661 atgcctatat tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg
    15721 gcaggtaacc agttcagaag ctgctgtcag agactctttt tttaatccac acagagacat
    15781 attgcccatt gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt tttttatttg
    15841 gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct
    15901 aaaagcccca ggcgttgctg taaccagtcg accagtttta tgtcatctgc cactgccaga
    15961 gtcgtcagca atgccatgac tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt
    16021 gtgtcacttt tcgtcggttg ttgtattaat gttgctaatt gatagcaata gaccatcact
    16081 gcctgcccca gattgagcga aggataatcc gccaccatcg gcacaccagt aagaacgtca
    16141 gccaacgcta actcttcgtt agtcaacccg gaatcttcgc gaccaaacac cagcgcggca
    16201 tggctcatcc atgaagattt ttcctctaac agcggcacca gttcaactgg cgtggcgtag
    16261 taatgatatt tcgcccgact gcgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac
    16321 gattcaacca gtgtcgggaa aactttaata ttatcaataa tatcaccaga tccatgtgcg
    16381 acccagcgag tggctggctc cagatgtgcc tgactatcga caatccgcag ttcgctaaac
    16441 cccatcgttt tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc
    16501 agaataatcg ttatacgcat attgccactc ttcttgacca aatgaccgcg aacagggtga
    16561 tcactgtcaa cttattacgc ggtgcgaatt tacaaatcct taacgtaagt cgcagaaaaa
    16621 gccctttact tagcttaaaa aaggctaaac tatttcctga ctgtactaac ggttgaattg
    16681 ttaaaaaatg ctacatatcc ttctgtttac ttaggataat tttataaaaa ataaatctcg
    16741 acaattggat tcaccacgtt tattagttgt atgatgcaac tagttggatt attaaaataa
    16801 tgtgacgaaa gctagcattt agatacgatg atttcatcaa actgttaacg tgctacaatt
    16861 gaacttgata tatgtcaacg aagcgtagtt ttattgggtg tccggcccct cttagcctgt
    16921 tatgttgctg ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg
    16981 aaagtaccca cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg
    17041 caattacgta ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag
    17101 gtcagggact tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca
    17161 gaccccgcac attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat
    17221 tttcgaagcg gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat
    17281 cctctctgaa tatgacatca acctggtgat catggatatc aatctgccgg gtaagaacgg
    17341 tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg
    17401 ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac
    17461 caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt cccgtaccat
    17521 gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg
    17581 ggaactggac atcaacagcc gttcgttgat cggccctgat ggcgagcagt acaagctgcc
    17641 gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtcccg
    17701 tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa ccacacgacc gtactgtaga
    17761 cgtgacgatc cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat
    17821 cgccaccatc cacggtgaag gttatcgctt ctgcggtgat ctggaagatt aatcggcgtt
    17881 accaccgtca aaaaaaatgg cgctttttag cgccgttttt atttttcaac cttatttcca
    17941 gatacgtaat tgatcgtcca ttgtaacttc tttactggct ttcattttcg gcagtgaaaa
    18001 cgcataccag tcgatattac gagtcacaaa catcatgccg gccagcgcca ccaccagcac
    18061 actggttccc aacaacagcg cgctatcggc agagttgagc agtccccaca tcacaccatc
    18121 cagcaacaac agcgcgaggg taaacaacat gctgttgcgc cagcctttca ataccgcctg
    18181 caaataaata ccgttcatta acgccccaat cagactggcg attatccatg ccacggtaaa
    18241 accgatatgt tcagaaagcg ccagcaagag caaataaaac atcaccaatg aaagccccac
    18301 cagcaaatat tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa caaagaacgc
    18361 cataaaagtc agtgcaatca gcagaatggc gtacttagtc gcccggtccg ttaattggta
    18421 ttgatcggct ggcgtcgtca ctgcgacgct aaacgcgggg aagttttccc agccggtatc
    18481 attgcctgaa gcaaaacgct caccgagatt attagcaaac cagctgcttt gccattgcgc
    18541 ctgaaaacct gactcgctaa cttcccgttt ggctggcaaa aaatcaccta aaaaactggg
    18601 atgcggccag ttgctggtta aggtcatttc gctattacgc ccggcaggca ccacagaaag
    18661 atcgccggta ccgcttaaat tcagggccat attcagcttc aggttctgct tccgccagtc
    18721 cccttcgggt aaagggatat gcacgccctg cccgccttgc tctaacccgg tgccgggttc
    18781 aatggtcagc gccgttccgt taacttcagg cgctttcacc acaccaatac cacgcgcatc
    18841 cccgacgcta atcacaataa agggcttgcc taagatgata tttggcgcgt tgagctcgct
    18901 aagacgcgaa acatcgaaat cggcttttag cgttaaatca ctgtgccaga cctgaccggt
    18961 ataaatccct atcttgcgtt cttccacgtt ctgattgcca tcaaccatca atgactcagg
    19021 taaccaaaaa tggataaaac ttcgtttccg ctccacggtt ttatcctctt cctgcaccgt
    19081 ataaagctcg gtcacaggaa tagcgataag cggcccaacg agtttttgcg gcccgctggt
    19141 actttggcga atcgcatctt ccacatcgct acggtaatcg gcacgttcga caatcacttg
    19201 ccgaatcagc attatcggaa tcaacaacag caatactgca ccaaacaagg tggtcatttt
    19261 ccagaacagg ggggatttca acatagtctt ctcctttgca atggcagcag gatacgaagg
    19321 ctatgtggga agaatttgaa gctatgtgaa gtgacggtga agtcgaagcg aggccagcac
    19381 gccaccttcc tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg cgacctcact
    19441 gacgaacgcc aaccccagac cgctgctttt ttgcccattt gcacgcggca aagagtaaaa
    19501 gcgttcaaaa atacgtgaca gcgcgtagtc aggaatacca ctaccggtat ccagcacctt
    19561 aagggcgacg tgttcctgat ccacttcggc gcttagcgtt atgcgaccgc tctcgggggt
    19621 aaaatcgatg gcgttatcca gtaaattccc cagcgcctgc tccagtaacg ccggttcagc
    19681 agcaacatta acctcagtgg gcataacatg tagagtgatg tttttttctg ccaactgcac
    19741 ggtgcgcgct tcgctgacgc ggcgaaataa tgccgccaca tcaacaacag tcagaacgac
    19801 ttcctgacga ttctccagtc ttgcctggcg tagtaacgtt tctaccaatg cctgcatacg
    19861 cgcattttgc gtcagaatgt tgtcggtaaa acgagccacc acttccggtg gcggaccttc
    19921 gcgtaaaatt tccgccgcgc cacgaatcgc cgccagtggg ctttttagct catgggttaa
    19981 cgcataaaca tactgctcaa tatagttttt cccttccagc ttcacgcgca tactttccag
    20041 cgcctgcgca agcttacgca actcgctact gccgagttca ggaagaggaa cgggcttatt
    20101 gtcagtgacg gaatcggcat agcgagtgag ccgggcaatg gagcggttga tccaccaaac
    20161 catgcctgcg ccaatcacca gtgcaatccc cagcaaaatg gcgctggccc ataaaattcg
    20221 ccgctcgcta cgcttaatga ccggagccat cgccgcgttc ggtttgccta cgctcaaaac
    20281 gccaataagc cgcgagccgt ccataatcgg cgcggcaaca tacatcaccg aactttcggg
    20341 atcggcagga ttttgcaacg tgctgcgcgc accatactga ccacgcaacg ttagccagac
    20401 gtcattccag cgcgaataat cctgcccaac ggctttattt gccgaatcga acaatacttt
    20461 accctgcgaa tcggtcatat agacatgata ttcattgcgc actttgttaa tgccaccgat
    20521 attggcgcga aacgggcgat gttgtagctg attaaacgcc tgcgccagtt gcccatgcgt
    20581 tgggtcccca gagagcaaat cgggacgcgc cagctccgcc agcaacgttg cggtatcgat
    20641 taacgtcccc tccgttgctc ttcgcacgcc cggtttaact tctttgacaa aaatagccag
    20701 tacgaaccag gccgccaccg ccaccagtaa aaaatagccc agcagcaacc gcatgccgat
    20761 acgcattaca ggcccctcag gctatatccc atgccgcgat gagtattaat cggtgaaaga
    20821 tcggggttga tggcgcgcag cttggcacgc aacgttttaa tgtgggtatc aatggtgcga
    20881 tcgtaggtgt cctgcgcatc ttcccacacg ctatccatta gttgctggcg ggaccagacg
    20941 cggcctggcg acttgagtaa cgtcttcagc aataaaaact cataccgcgt cagcgttaat
    21001 ggcgtgtcaa accagctgat ctgcgccgcg ggttcattca attcaaaatg accaatacgg
    21061 atgacgggag acggcgacga gaacttcttc acccgacgca gtaaggtgcg cacccttgcg
    21121 cacacttcgc ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat ttccagccca
    21181 agcaggcgat cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa cgccggatgg
    21241 agcgccagta actggcggca taattcaaag ccgctgatat caggcaaacc aacatcgaga
    21301 atcatgacgt cgggagcctg ctggcgagct ttatccagca ccggcaagcc tcgctcaaag
    21361 acctcgacgg caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc tatcccttgc
    21421 tcatcttcca ctaaccagac cgtttcccgt tgcataaatc gcctctgtta ttgccgccac
    21481 ggcatgacag gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac tttgtcggag
    21541 taagccagat aagcgagcgc gttgcgtttg gcgtcataaa agcgcacgac ctgtaacgac
    21601 ttaaagacca gggacgtgcg ttttttgaat actacctcgc cctgagcttt gccgttttta
    21661 atacgatccg acagttcaat cggcccgact tgctgacaag aaatagccgc atcggaggta
    21721 tcttccgcca gacccaatcc ccctttaata cccccggttt tcgcccggct cacataacag
    21781 gtgacatttt tcacatcggg atcgtcaaag gcttccacaa caattttgtg atccgggccg
    21841 atcattttaa atacggtgtc gaccgaacca atctcctctg catgagccaa tggccccagc
    21901 attatcaggc ttaaagacag gatcaaatgc ttgtatttca tattgttacc atttttatct
    21961 attacacgag caattagttc gtcacggtaa ataacatatt gattaagaac aaaatctcaa
    22021 tacttttatt tccgtcaagc cctaaaacat actctactaa gaaaaaaaca ctgaatgcta
    22081 aaacagcaaa aaatgctatt atccaattac ctgatgtcag gtgctcgttg ttgaaaggat
    22141 gaggatattt tatggatcag gccggcatta ttcgcgacct tttaatctgg ctggaaggtc
    22201 atctggatca gcccctgtcg ctcgacaatg tagcggcgaa agcaggttat tccaagtggc
    22261 acttacagag aatgtttaaa gatgtcactg gccatgctat tggcgcgtat attcgtgctc
    22321 gtcgtttgtc gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt ctggacatcg
    22381 cgctgcaata ccgcttcgac tctcaacaga catttacccg cgcattcaag aagcagtttg
    22441 cccagactcc tgcactttac cgccgttctc ctgaatggag cgcctttggt attcgcccgc
    22501 cgctacgtct gggtgaattc actatgccag agcacaaatt tgtcaccctg gaagatacgc
    22561 cgctgattgg tgttacccag agctactcct gttcgctgga gcaaatctct gatttccgcc
    22621 atgaaatgcg ttatcagttc tggcacgatt ttctcggcaa cgcgccgacc attccgccgg
    22681 tgctctacgg cctgaatgaa acgcgtccga gtcaggataa agacgacgaa caagaagtat
    22741 tctataccac cgcgttagcc caggatcagg cagatggcta tgtactgacg gggcatccgg
    22801 tgatgctgca gggcggcgaa tatgtgatgt ttacctatga aggtctggga accggcgtgc
    22861 aggagtttat cctgacggta tatggaacat gcatgccaat gcttaacctg acgcgccgta
    22921 aaggtcagga tattgagcga tactacccgg cagaagatgc caaagcagga gatcgcccaa
    22981 ttaatctgcg ctgtgaactg ctgattccga tccgtcgtta acgctgcaac tcatctaatg
    23041 caggggcgtc cagatgcgag atgtcccctg cggtttcaac aacccagcca gacgccagcc
    23101 acaggctttc ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt aagcggcgct
    23161 ctgcccatgc tggtaatccg agaatcgtac tcaccaggca tcccagtgca ataccatgac
    23221 tcaccagcag cggtcggctt ccctgcggta agtcccggca agattccagt gccgcattga
    23281 cgcgatcgct gagctcctgc attgactcgc cttcaggaat acgcccgtca acggtgccat
    23341 tgaccagctg ccgacgccag ttctcttctt cttcggtcag agaatcgata tgtctttttt
    23401 ccagcacacc catgtttaat tcacgcaggc gagaatcaaa gatgatgtcg cagccgcagg
    23461 cctgggcgat gatttccgcc gtacgccggg tgcgtcctaa atcgctactg atgatatgcg
    23521 taatgccaag ctctttggca cgggttgcca cctgcatcgc ctgttgctca cctttggcgg
    23581 tcagcgggct gtcagactgg ccctgaatac gtcgctcggc gttccactgc gtttcaccgt
    23641 ggcggactag gtatacctgt aacatgcttt ttttccgtat actgctgtaa cgttaatttt
    23701 ctgagtaatg ctgattatgc accaagttgt ctgtgcgacc accaatcccg ctaaaattca
    23761 ggccattctg caggcatttc acgagatctt cggcgaagga tcctgccata ttgcatccgt
    23821 tgccgtcgag agcggtgtac cggaacaacc ctttggcagt gaggaaacgc gcgctggcgc
    23881 acgaaatcgg gtagcaaatg cccgccgttt acttccagag gctgattttt gggtggcgat
    23941 tgaagctggc atcgatggtg acagcacttt cagttgggtg gtgattgaaa acgccagcca
    24001 gcgcggcgag gcgcgttcgg ctactttgcc gttgcctgcg gtgattctgg aaaaagtgcg
    24061 tgaaggcgag gcgctcggcc ccgtgatgtc gcgttatacc ggtattgatg agattggtcg
    24121 taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc acccgcgcca gcgtctatca
    24181 ccaggcggtg atcctggcgc tgagtccgtt tcataatgcc gtgtatcagg cattgtagga
    24241 cggataaggc gttcacgccg catccggcat tcggtgcacg atgcctgatg cgacgctgcc
    24301 gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa cacctcttcc agccactggc
    24361 gcagctcaac gggcgcggct ttcaggctgt tagatccacg cgtaatcgtc gcgatgcccg
    24421 cgccgagttc attttttaac tcacgctggc tcatttcgcc gcgcaacagc tcttcgacaa
    24481 tacgcacgcg agtccccaac gcttcgcgct catctggcgt cagcatcagg tttaacaacg
    24541 gtaaatggag atcgttttgg taggcattct taagcaggtc gacaaaacgt aaccactcct
    24601 ggtgacgctg ttctgccatc gctgctgaat agggtgattg ttgggccata atatgtcgcc
    24661 attgttagcg ggggaagcaa aatgcctccc ccggttgtac tcgctaaaga gtacgatagc
    24721 atatcataaa cgtgcggatc agtaacgacg tccccattcc gtggtgctca tcaacgtcgg
    24781 tttatccccc atgaaatagc ggtagtaagc gtcataagcc agcacgttct tcacataacc
    24841 gcgcgtctcg gagaacggaa tactctcgac aaatgccaca gcgtcgatac gcccggcgct
    24901 gttgccaagc caggttcgca cccgtccagg tccggcgtta taagctgctg aggagaaaat
    24961 acggttattg ccaaactgct gataaacata ttgcaggtaa ctggtgccaa tgttgatatt
    25021 cgtttccgga tccagcaatt gcccagggct gctataacca ggaatagaga acatcttcac
    25081 cgtatgggtc gctgtaccag gcataatctg catcaggccg ctggccccta ccggtgattt
    25141 cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc atcgcatagc tttgcgggat
    25201 ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa gccagcggga atcgctcttc
    25261 cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg ctaagatccc accattggtt
    25321 attgaaagca taccgagcca gttgagcctg ctctgttttt gacttgctct tcaccagatt
    25381 ggcccactcg ctacgcgcgg tgttatcgag attccagtac atcaactcgc gcacgcgcgc
    25441 catctccggc ccctgagtca gggcgctgtc aacattctgc ggcgctttat caatcttcag
    25501 ctcatactct tctccgatgc gttgtgctgc aaccatcggg tagaaaccac gctgttgcat
    25561 gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg cgttccagca ataaatccgc
    25621 ctgccagtaa cgccattcat ctttctcttt tgcttccatc ggcagacgcg ccagccaggt
    25681 attcaggccg cgacgatcgc cggtgccaag tgccattcgc acacggcgtt caatcaacga
    25741 cgtggattgc gagcgcataa tggcgtcatc gcgccatttc gcctgctcgt cggtgacatc
    25801 gttgcccatc aaacgccagg cgacgatatc gcgcagctcc tgaatatgat cttcattaag
    25861 ctgctgcgcc tgggcaagcg acgggatcat cagccgtgca ttttcagcat cctgccgcgc
    25921 cacactggca aacgccaccg ccgccatttg acgggtaaaa tcggtcgctc cggttgtacg
    25981 cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa atgattgccg aggcgatagt
    26041 ctggtaatcg gcaggcatct gccctgccag cactgttacc aggccagtgt tacccgcttt
    26101 catcgccaga cggatacgct ctaaataagc cagcggatct tgtttacctg acgcacgcca
    26161 gacgctaaat aacttgtcac aggcgttagg ctggctcttg ccggttagcc acagctcttt
    26221 cgccccttgc caggcttctt cactctgccc ggtgttccat ttcgcatagt agtaattaca
    26281 ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct aacaagccac gccagtcttc
    26341 acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg cgagcgggag gaagcgtggg
    26401 gttagcgcga acaaagttag tgaccgtcac cgccggttga ttcatcagat catcggtgat
    26461 ctggcggtat tccaggtagg gataaagcgg ataatccttc agtccaggca tcatttgttc
    26521 gaccacatcc atttgtcgat tatcccaggc ctgcttgatt tgcgcgtaac ggctacgctg
    26581 ctcatccagt gagtcggctc gcgccacgct gctgaccgtc agcagacaga caccggcagc
    26641 caacagccgc caggtaactt gtttggcttt ttccacaagc gcatcctcta agtgtaaata
    26701 catcaatgca gcgtcatgcc gcgtaataga tttatgctaa ccagtcattg ccgtttacgc
    26761 cacgttacgg acactttttt acttttactg cgaaggtgat cgaccaactg ggattaggcg
    26821 gggaaaaagg ctacacttcg cctttgaaaa ctcatcatca cgataaacat agaggcgaag
    26881 tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg gcaaagttgt tccgccgaaa
    26941 cgtcatattt tgaaaaacat ctctctgagt ttcttccctg gggcaaaaat tggtgtcctg
    27001 ggtctgaacg gcgcgggtaa gtctaccctg ctgcgcatta tggcgggcat tgataaagac
    27061 atcgaaggtg aagcgcgtcc gcagccagac atcaagattg gttacctgcc gcaggaaccg
    27121 cagctgaacc cggaacacac cgtgcgtgag tccattgaag aagcggtttc tgaagtggtt
    27181 aacgccctga aacgcctgga tgaagtgtat gcgctgtacg ccgatccgga tgccgatttt
    27241 gacaagctgg ccgctgaaca aggccgtctg gaagagatca ttcaggctca cgacggtcat
    27301 aacctgaacg tacagctgga gcgtgcggcg gatgcgctac gtctgccgga ctgggacgcg
    27361 aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtag cgttgtgccg cctgctgctg
    27421 gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc acctggatgc cgaatccgtg
    27481 gcctggctgg aacgcttcct gcacgacttc gagggcaccg tggtggcgat tacccacgac
    27541 cgttacttcc tcgataacgt tgcaggctgg atcctcgaac ttgaccgcgg tgaaggtatt
    27601 ccgtgggaag gcaactactc ctcctggctg gagcagaaag atcagcgcct ggcgcaggaa
    27661 gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag agctggagtg ggtacgtcag
    27721 ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg cacgctttga agagctgaac
    27781 agcaccgaat atcagaaacg taacgaaacc aacgaactgt ttattccacc tggaccgcgt
    27841 ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat cctacggtga tcgcctgctg
    27901 attgatgacc tgagcttctc gatcccgaaa ggggcaatcg tcgggatcat cggtccgaac
    27961 ggcgcgggta aatcgaccct gttccgtatg atctctggtc aggaacagcc ggacagcggc
    28021 accatcactt taggtgaaac ggtgaaactg gcatcggttg atcagttccg tgactcaatg
    28081 gataacagca aaaccgtttg ggaagaagtt tccggcgggc tggatattat gaagatcggc
    28141 aacaccgaga tgccaagccg cgcctacgtt ggccgcttta actttaaagg ggttgatcag
    28201 ggtaaacgcg ttggtgaact ttccggtggt gagcgcggtc gtctgcatct ggcgaagctg
    28261 ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa ccaacgacct ggatatcgaa
    28321 accctgcgcg cgctggaaaa cgccctgctg gagttcccgg gctgtgcgat ggttatctcg
    28381 cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc tggactacca ggatgaaggt
    28441 aaagttgagt tcttcgaagg taactttact gagtacgaag agtacaagaa acgcacgctg
    28501 ggcgcagacg cactggagcc gaagcgtatc aagtacaagc gtattgcgaa gtaatgcgta
    28561 aaatgccgga tgcggcgtga acgccttatc cggcctacaa accaagaaat ttcaatacgt
    28621 tgtgaatctc caggcctgat aagacgcggt aagcgtcgca tcaggcattg agcaccgaat
    28681 gccggatacg gcgtgaacgc cttttccggc ctacaacagc cttagtatcc gcaatttcgt
    28741 agcgggtcac ccgtgcaggt tttttcaaac cttgtttaat ctcgacagcc tcttccagaa
    28801 aggttttcaa ctcatcaaaa aacttatttt atacctcgcc tttgagccgt ttcatcgcgg
    28861 ttatctctgc tcccccatca tctcccgcac cagctccacg cagcgcagga aacggctgtc
    28921 ataatcgtcc tcttcaaccc gcacgaattc gatattgttc tcttccagca tctccaccag
    28981 caagttctgg aactctttgc gatccaccga actgccgagg ctgcgtaaac catccgccac
    29041 ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcaacgc
    29101 ctgcacgaac ggatgctcac gcccttcgta ctttttgcag aacgcctggg tagtgacaaa
    29161 atcagtgtca ataaacgcca ctttattggc gtatttcact gcaaaatcaa tgtattgtgc
    29221 gtggcccagc gcgattttat cgtagtcgga atactgcaat gcgatctcat caccgccgag
    29281 gtgtgaaaag acataatcgc gaccatattc ccacgcactg gtggtattga agatattggc
    29341 aagtttgttt accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg
    29401 cacaaagaac ggcttcactt ccgtaggaat atattcccag tagcggaacg ggttttcgcg
    29461 gatctgcgca ccgctgatac tcataaaggt acgtttcgga tcgaccagta ccgtctcgat
    29521 ccccagatgt tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg
    29581 ctggatcccc ttttcagcca taaacttttt gatgccgttg ctccacacat cccagccatg
    29641 cggatacggc tccatgccct cttcgttgaa agcatgaatg cgaatatttt tctgatattt
    29701 aaaagtttgc aataaccagc gcagacgatc cggcaccgtg ggctgctgcg acatggcgct
    29761 gtcttcaaac aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc
    29821 aacctggcta caggcgcgct ggataaggta gatatgtccg gtatgcagcg ggtagaactt
    29881 accgaatacg acaccaatgg ttttcttctg ccggggaaat tcaagcccca aaaaacggtg
    29941 caacgcctcc agcttttgcg cgctggggct tttaattttg gcattcagta actggcttaa
    30001 ataccctttg gtcataccgc tggcatcagc tacctgctgt aacgtgcagc cctgttgctt
    30061 gatggcagtt ttcaggtaat caaatgacga catatctccc tccgtatctc tcattataag
    30121 tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc
    30181 ggcgcttttt tcggcacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc
    30241 gcttcagaga ttcgctcctg accgctgggc accgggcgga tctcccctgc cagcccgact
    30301 tcaccaaaca ccaccagatc ctgcggtagc ggtctgtcac gcaggctgga aaccatcgcc
    30361 agcagtaacg ctaagtcggc actggtttcg gttaccttca caccaccgac cacgttcaca
    30421 aatacatcct gatcggccat ttgcagacca ccgtgacggt gcaagacagc cagcaggatt
    30481 gccagacggt tttgctccag acctaccgcc acgcggcgcg gattcgccat catcgagtga
    30541 tcgaccagcg cctgaatctc caccagcaac ggacgcgttc cttcccacac caccatcacg
    30601 gagctgccgg aggtcacttc atctccgcga cttaagaaaa ttgccgaagg gttgctgact
    30661 tcacgcagcc cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg
    30721 tttttatggc tgcgcaaggt gcgaaaacgg gagtcggcat cgccatccaa aagcaccgaa
    30781 cagtcgatac agtgttccag cactttaggg cccgccagcg agccatcttt ggttacgtgt
    30841 ccaaccatga caatcgccac accgcgcgtt ttggcgaagc gtgtcaaata agccgccgtt
    30901 tcacgcacct gcgccacgct accaggcgat gactgtacat ccgccatatg catcacctgg
    30961 atggagtcaa ttaccatcag cttcggttgc tcttcttcag caatcaggca gatctgctcg
    31021 atgctggttt ccgacaacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc
    31081 attgccacct gttgcagcga ctcttcgccg gtgacataca gcgttttcat ctgctgggcc
    31141 agtttgcaca gcgtttgcag caacagcgtg gatttccccg cgcctgggtt accgccaatc
    31201 agaatggcac ttcccggtac cacgccgccg cctaatacgc ggtcgaactc tttaaatccg
    31261 gtggaaaaac gcggcagctc ttcaaggctg atgtcggaga gtttctggac ttttgccacc
    31321 ccggcgctac cggcatagcc actgagacgc tcgttacgcg ccaccgttgg cgacgcagca
    31381 agacgcacct cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc
    31441 ggataatcgg ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg
    31501 tacctcgtta atgctgtgcc gcccgcagga tgacgggcag gcaattactt ctgattcagg
    31561 ctgccagaga ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgtc
    31621 ttttcattca cttttggctt ggcatggtag gcaatcccca gccctgccgc tttgatcatc
    31681 ggcaggtcat tggctccatc gccaatcgcc acggtctgcg ccagcgggat ttcatactcc
    31741 tgcgcgaggc gggtcagagt tttcgctttg tactgcgcgt caacgatgtc gccgatcaca
    31801 ttgccggtaa atttaccgtc catgatctcc agttcattgg ctaccacggc ggtcaggcgc
    31861 agcttgtcgc gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc
    31921 cagcccagcg tttccagctt gagcaccagc tgcgttaagc ctggcatcag cggcagattt
    31981 tcacgcacct gttgcagaat attggcatca gcgcctttca gcgtcgccac gcggctgcgc
    32041 aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt cggttacctc cgccaccatc
    32101 tcgcccgttc cggccagttt ggcaatttca tcaatacatt caatctggat ggcggtggaa
    32161 tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc cagcggcgcg
    32221 acatccagtt gcgcttcatg ggccaggcgc gtagcgcgcg cggtgagtga acctgccaga
    32281 cgaatcacct gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcgcccagt
    32341 ttgctctggt attgggtcag gcgttgttta tccagcccac gaccatacag cagccagccg
    32401 ctacgacctg cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga
    32461 cccggccata aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct
    32521 cctgtaaaat cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt
    32581 ctggcaacat taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat
    32641 tccggctgca tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc
    32701 agggggcgtc atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac
    32761 tggcccgcac cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact
    32821 caccagatga aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa agccgcatcc
    32881 tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc ccgttcaggc gaaagcgtcg
    32941 aagtgcgcga ccggctggcg ctcgacggta aaaaagcagg cggctatttt aaccagcaga
    33001 ttgtcgagcc aattgcgggt aaaaacggac cgctcggcta tctgcgcctg acactcgaca
    33061 cccatacgct cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga
    33121 tgttgctgct ctcactggca atcggcgtag tgctgacccg cacgctgcta cagggtaaac
    33181 gcacccgctg gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg
    33241 aagaaagcga gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt atgtccacat
    33301 tacgcctgct catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta
    33361 tttttcgcca aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg
    33421 tagtgattgg tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg atggaagaag
    33481 ataacgtccg cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac gatctcggca
    33541 atacctgctt tacctttatg gctggcaagc cggagtacga taaaaccatc tctacgtcga
    33601 ttgtgctcaa tgcgctgaac gcgctcggcg tcagcgccga agcgtccggg cgtaacgatc
    33661 tggtggtgaa aacagtcgaa ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca
    33721 aagatcgtgg cttccaccac ggcactttgc tgctcaatgc cgaccttagc cgcctggcaa
    33781 actatctcaa tccggataaa aagaaactgg cggcgaaagg cattacctca gtgcgttccc
    33841 gcgtgaccaa cctcaccgag ctgctgccgg ggatcaccca tgagcaggtt tgcgaggtca
    33901 taaccaaggc ctttttcgcc cattatggcg agcgtgtaga agcggaaatc atctccccgg
    33961 acaaaacgcc agacttgcca aacttcgccg aaacctttgc ccgtcagagt agctgggaat
    34021 ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt agctggggcg
    34081 gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag gtgtttaccg
    34141 acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc tgcctgtacc
    34201 gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg gaccaggaaa
    34261 aagagctacg ggagttgtcg acgtggatag cgggggcggt aaggtaactc gctgattaaa
    34321 ataccaaagg aagggaatat gatcgatctg gaaaaccagg agcgggagat tatcaattta
    34381 atgttaagcc agcgtatttc ctggctagcc gccgtgcgta tcaggcataa attatcgctt
    34441 gctgaagtaa gtaaaatgtt aggtatctcg attaattctc taaaacagat cgaaaagacg
    34501 gaacggctta gctcaaacat caaaagcaaa atggcagaaa tttatggttg tccaccagag
    34561 ttgctgatat gcccttcctg gatgacggct gaacataaat gaatagat
//
LOCUS       DAZFJF010000040        33213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFJF010000040 DAZFJF010000000
VERSION     DAZFJF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.040"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1168)
                     /locus_tag="ACHKAU_002117"
     CDS             complement(<1..1168)
                     /locus_tag="ACHKAU_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5531181.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDIARKQVANGIDPVEERKAQKLAQKLSTKNSFES
                     VSREWHAAKADRWTLAYREEIIKTFEQDVFPFIGKRPIAEIKPLELLEVLKRIEKRGA
                     LEKTRKVRQRCGEVFRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFVRD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFATWQELDLEKGLWEIPAERMKMRRPHIVP
                     LSRQVIRLFQQLKPITGHYPYIFIGRNNRKKPISKESINQVIELLGFKGRITGHGFRH
                     TMSTILHEAGFNSAWIEMQLAHVDKNSIRGVYNHAQYLNGRSRMLQFYADKLYPN"
     gene            complement(1331..1425)
                     /locus_tag="ACHKAU_002118"
     tRNA            complement(1331..1425)
                     /locus_tag="ACHKAU_002118"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1389..1391),aa:Sec,seq:tca)
     gene            1718..3100
                     /locus_tag="ACHKAU_002119"
     CDS             1718..3100
                     /locus_tag="ACHKAU_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO5531182.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3110..5428
                     /gene="yicI"
                     /locus_tag="ACHKAU_002120"
     CDS             3110..5428
                     /gene="yicI"
                     /locus_tag="ACHKAU_002120"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO5531183.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5481..7190)
                     /locus_tag="ACHKAU_002121"
     CDS             complement(5481..7190)
                     /locus_tag="ACHKAU_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5531184.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(7311..8702)
                     /gene="xanP"
                     /locus_tag="ACHKAU_002122"
     CDS             complement(7311..8702)
                     /gene="xanP"
                     /locus_tag="ACHKAU_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO5531185.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8982..10187
                     /gene="gltS"
                     /locus_tag="ACHKAU_002123"
     CDS             8982..10187
                     /gene="gltS"
                     /locus_tag="ACHKAU_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO5531186.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10190..11056
                     /locus_tag="ACHKAU_002124"
     CDS             10190..11056
                     /locus_tag="ACHKAU_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531187.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11041..13122)
                     /gene="recG"
                     /locus_tag="ACHKAU_002125"
     CDS             complement(11041..13122)
                     /gene="recG"
                     /locus_tag="ACHKAU_002125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO5531188.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13128..13817)
                     /gene="trmH"
                     /locus_tag="ACHKAU_002126"
     CDS             complement(13128..13817)
                     /gene="trmH"
                     /locus_tag="ACHKAU_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO5531189.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13824..15932)
                     /gene="spoT"
                     /locus_tag="ACHKAU_002127"
     CDS             complement(13824..15932)
                     /gene="spoT"
                     /locus_tag="ACHKAU_002127"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO5531190.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(15951..16226)
                     /gene="rpoZ"
                     /locus_tag="ACHKAU_002128"
     CDS             complement(15951..16226)
                     /gene="rpoZ"
                     /locus_tag="ACHKAU_002128"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO5531191.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16281..16904)
                     /gene="gmk"
                     /locus_tag="ACHKAU_002129"
     CDS             complement(16281..16904)
                     /gene="gmk"
                     /locus_tag="ACHKAU_002129"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO5531192.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17162..18844
                     /gene="ligB"
                     /locus_tag="ACHKAU_002130"
     CDS             17162..18844
                     /gene="ligB"
                     /locus_tag="ACHKAU_002130"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO5531193.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18841..19458)
                     /locus_tag="ACHKAU_002131"
     CDS             complement(18841..19458)
                     /locus_tag="ACHKAU_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO5531194.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19750..20574)
                     /gene="dinD"
                     /locus_tag="ACHKAU_002132"
     CDS             complement(19750..20574)
                     /gene="dinD"
                     /locus_tag="ACHKAU_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO5531195.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20795..21658)
                     /locus_tag="ACHKAU_002133"
     CDS             complement(20795..21658)
                     /locus_tag="ACHKAU_002133"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO5531196.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21785..22501
                     /gene="rph"
                     /locus_tag="ACHKAU_002134"
     CDS             21785..22501
                     /gene="rph"
                     /locus_tag="ACHKAU_002134"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO5531197.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22567..23208
                     /gene="pyrE"
                     /locus_tag="ACHKAU_002135"
     CDS             22567..23208
                     /gene="pyrE"
                     /locus_tag="ACHKAU_002135"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO5531198.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23245..23841)
                     /gene="slmA"
                     /locus_tag="ACHKAU_002136"
     CDS             complement(23245..23841)
                     /gene="slmA"
                     /locus_tag="ACHKAU_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO5531199.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23948..24406)
                     /gene="dut"
                     /locus_tag="ACHKAU_002137"
     CDS             complement(23948..24406)
                     /gene="dut"
                     /locus_tag="ACHKAU_002137"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO5531200.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24384..25604)
                     /gene="coaBC"
                     /locus_tag="ACHKAU_002138"
     CDS             complement(24384..25604)
                     /gene="coaBC"
                     /locus_tag="ACHKAU_002138"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO5531201.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25776..26444
                     /gene="radC"
                     /locus_tag="ACHKAU_002139"
     CDS             25776..26444
                     /gene="radC"
                     /locus_tag="ACHKAU_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5531202.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26661..26897
                     /gene="rpmB"
                     /locus_tag="ACHKAU_002140"
     CDS             26661..26897
                     /gene="rpmB"
                     /locus_tag="ACHKAU_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO5531203.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            26918..27085
                     /gene="rpmG"
                     /locus_tag="ACHKAU_002141"
     CDS             26918..27085
                     /gene="rpmG"
                     /locus_tag="ACHKAU_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO5531204.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27183..27992
                     /gene="mutM"
                     /locus_tag="ACHKAU_002142"
     CDS             27183..27992
                     /gene="mutM"
                     /locus_tag="ACHKAU_002142"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO5531205.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28031..28510)
                     /gene="coaD"
                     /locus_tag="ACHKAU_002143"
     CDS             complement(28031..28510)
                     /gene="coaD"
                     /locus_tag="ACHKAU_002143"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO5531206.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28518..29795)
                     /gene="waaA"
                     /locus_tag="ACHKAU_002144"
     CDS             complement(28518..29795)
                     /gene="waaA"
                     /locus_tag="ACHKAU_002144"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO5531207.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30208..31266
                     /gene="rfaQ"
                     /locus_tag="ACHKAU_002145"
     CDS             30208..31266
                     /gene="rfaQ"
                     /locus_tag="ACHKAU_002145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO5531208.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31263..32387
                     /locus_tag="ACHKAU_002146"
     CDS             31263..32387
                     /locus_tag="ACHKAU_002146"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5531209.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            32380..33177
                     /gene="rfaP"
                     /locus_tag="ACHKAU_002147"
     CDS             32380..33177
                     /gene="rfaP"
                     /locus_tag="ACHKAU_002147"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO5531210.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7836 a         8223 c         8852 g         8302 t
ORIGIN      
        1 agtttgggta tagtttatct gcataaaact gaagcatacg agatcgacca ttaagatatt
       61 gtgcatgatt gtatacaccg cgaatgctgt tcttatcaac gtgggcaagt tgcatttcaa
      121 tccatgcact attaaaaccg gcttcgtgta gtatggttga catagtgtgt ctgaaaccat
      181 ggccagtgat tctcccctta aatcccaata actcaatcac ttggtttatg ctctccttac
      241 ttataggctt ttttcggtta tttcgtccaa tgaaaatgta aggatagtga ccagtgatcg
      301 gttttagttg ttgaaatagc cttataacct gccttgaaag cgggactatg tgtggcctac
      361 gcatcttcat acgctctgct ggtatctccc atagcccttt ttctaaatca agctcttgcc
      421 atgtagcaaa gcgcatctct tgtgttcgaa cacctgtaag catcacaatc ttggttgcat
      481 ttttagtgat tatgctgcca gtgtatgcct caagatctct gacaaaatgt gggagttcct
      541 ctgctgatag aaaaggatga tgtttttgct tcggcactgc taatgcgata gctaaatcag
      601 gtgctggatt gtattcagcg cggccagtga tgatcgcgta gcgaaacact tctccgcacc
      661 gttggcgtac ttttcttgtt ttctccaacg ctccacgttt ttctatgcgt ttaagtactt
      721 caagcagttc taggggttta atttcagcta ttggtcgttt accaatgaaa ggaaaaacat
      781 cctgttcaaa tgttttaata atttcttctc gataagctaa ggtccaacga tctgcttttg
      841 ctgcatgcca ttctctgcta acgctctcaa aagagttttt agtggataat ttctgagcaa
      901 gtttctgagc tttacgttct tctaccgggt caatgccatt agcaacctgc ttacgggcga
      961 tatcacgctt ttcacgtgct tctgcgaggc ttactaaatc gtagctgcca aatgacatta
     1021 aacgcgcttt ccctgcaaac cggaaacgga aacgccagcc tttcgagcca tcaggattga
     1081 taagcaatga cagaccttgt ccatcgttca atgtgtatgg tttgtcttgt ggttttgcac
     1141 gtttgatctg tatatcggtt agagccatgt gtataactga aaactgcgta taaaaaatct
     1201 atacacatta ctatacatat ttttcgcgga ttcagggaga tgatctcgga ctattacgga
     1261 taagctgatt attcatcgca ttgaatataa aggattttat ggagtgcctc ggaactggct
     1321 ggatgaggtt tggcggaaga tcacaggagt cgaacctgcc cgggaccgct ggcggcccca
     1381 actggatttg aagtccagcc gcctcaccgg agacgacgat cttccgcgcc tcgattgcta
     1441 catggaggcg gggcgcatta tagctacttc cttgagtttc tacatccccc agatcagttt
     1501 tttccgccta atacacgcag cgtattatca cgaaagcatt atagttcata taaaacctat
     1561 attttacaat gatatatgag cgaaattaac gctaccacga tcacataaaa caagaatcat
     1621 aaattaataa ccagatatcg gaatattcgc tctcacaggg atggtttaca aaatgcgtta
     1681 tcggtctcag cacccctata gcatcaagga aaagcagatg aagagtgaag tgttgtccgt
     1741 taaagagaaa attggttatg gcatgggaga cgccgccagc cacattattt tcgataacgt
     1801 aatgttatat atgatgttct tttataccga tatttttggc attcctgccg gttttgtcgg
     1861 aaccatgttt ttggtcgctc gtgcactgga tgcgatttcc gatccttgca tggggttgtt
     1921 ggccgatcga acgcgctctc gctggggtaa atttcgtccg tgggtactgt ttggcgcact
     1981 gccattcggg atcgtctgtg tactggccta tagcacgcca gatctcagta tgaacggcaa
     2041 aatgatctat gcagcaatta cttacaccct acttacctta ctttataccg tcgtcaatat
     2101 cccttactgc gcattgggtg gtgtaatcac caatgacccg actcagcgta tctcgctgca
     2161 atcctggcgt tttgtgctgg cgaccgcggg aggcatgctt tctactgttc tgatgatgcc
     2221 actggttaat ttaattggcg gtgataataa accactcggt ttccagggcg gtatcgcggt
     2281 cctttccgtg gtggcattca tgatgctggc attctgtttc ttcaccacta aagaacgcgt
     2341 tgaagcacca cctacaacaa cgtctatgcg ggaagattta cgtgatatct ggcaaaacga
     2401 ccagtggcgg attgtcggtt tactaaccat tttcaatatc ctggcggtgt gcgtacgcgg
     2461 tggggcgatg atgtattacg tcacatggat tttgggcacg ccggaagtgt ttgtcgcttt
     2521 tctcaccact tattgcgtgg gtaacctgat tggttccgca ctggcaaaac ctctgaccga
     2581 ctggaaatgt aaagtcacta tcttctggtg gacgaacgcc ctgctggcag tgattagcct
     2641 cgcgatgttc tttgttccca tgcaggccag catcactatg tttgtcttca tcttcgtgat
     2701 tggtgtgttg catcaactgg tgacacctat ccagtgggta atgatgtccg ataccgtcga
     2761 ctacggcgag tggtgcaatg gtaaacgcct gaccgggatc agttttgctg gcacgctgtt
     2821 tgtgctcaaa ctggggttgg ccttcggcgg cgctcttatc ggctggatgc tggcttatgg
     2881 cggatatgat gcggcagaaa aagcgcagaa cagcgccacg attagcatca ttattgcgct
     2941 attcacgatt gttccggcga tctgttattt gctgagcgcg attatcgcta aacgctacta
     3001 ctcactcacg acgcacaatc tgaaaaccgt tatggaacag ctggctcagg gtaaacgccg
     3061 ttgccagcaa caattcacct ctcaagaagt gcagaactaa ggaacggcaa tgaaaattag
     3121 cgatggaaac tggttgattc aacctggcct caatttgatt cacccgcttc aggtattcga
     3181 ggttgaacag cagggtaatg aaatggtggt ctatgctgcc ccccgtgatg tgcgtgaacg
     3241 tacctggcag cttgatacgc ctttatttac gctgcgcttt ttctccccac aggaaggtat
     3301 tgtcggtgtg cggattgagc attttcaggg ggcgctgaat aacggtcctc attatccgct
     3361 caatattttg caggacgtga aggtcacaat cgaaaacaca gaacgttatg ctgagtttaa
     3421 aagtggcaac ttaagcgcgc gtgtcagcaa aggtgagttc tggtcactgg attttctgcg
     3481 caacggcgaa cgtattaccg gtagtcaggt gaaaaataat ggctacgtgc aggacacgaa
     3541 taatcaacgc aattatatgt ttgagcggct tgatcttggc gttggcgaaa cagtttacgg
     3601 tctgggagag cgctttactg ccctggtgcg caatggccag acggtagaga cctggaaccg
     3661 ggacggcggc acaagtactg aacaggcgta taaaaatatc ccgttctaca tgactaaccg
     3721 tggttatggg gtactggtca atcatcccca gtgtgtctct tttgaagtgg gatcggagaa
     3781 agtctccaaa gtgcagttca gcgttgagag tgaatatctc gaatactttg ttatcgacgg
     3841 cccgacgccg aaagcggtac ttgatcgtta tacccgcttt actggtcgtc cggcgctgcc
     3901 gcccgcgtgg tccttcggcc tgtggctaac cacttcattt accaccaact acgacgaagc
     3961 gacggtaaac agctttatcg atggtatggc ggaacgcaat ctgccgctgc atgttttcca
     4021 ctttgactgt ttctggatga aagccttcca gtggtgcgat tttgagtggg acccgctgac
     4081 tttccctgac ccggaaggga tgatccgccg cctgaaagcg aaagggctga aaatctgcgt
     4141 ctggattaac ccctatatcg gtcaaaaatc ccccgtcttt aaagagttac aagagaaagg
     4201 ctatttactc aaacgcccgg acggttcgct atggcagtgg gataaatggc agccaggtct
     4261 ggcgatttat gactttacca atccggatgc ctgcaaatgg tacgccgaca aactgaaagg
     4321 tctggtcgcg atgggcgttg attgctttaa gaccgacttt ggcgaacgta tcccaactga
     4381 tgttcagtgg tttgacggtt ccgatccgca gaaaatgcat aaccattatg catacatcta
     4441 caacgaactg gtgtggaacg tgctcaagga caccgttggt gaggaagaag ctgtcttgtt
     4501 tgcccgctcg gcctccgtcg gtgcgcagaa attcccggta cactggggtg gcgattgtta
     4561 cgctaactac gaatcaatgg cggaaagcct gcgcggtggt ttgtctattg gcctttcagg
     4621 ttttggcttc tggagccacg atatcggcgg ctttgaaaat accgctccgg cgcacgttta
     4681 caaacgctgg tgcgcgtttg gtttgctctc cagccatagc cgtttacacg gtagcaaatc
     4741 ttatcgtgtg ccgtgggcct acgatgatga gtcctgtgat gtagtgcgct tcttcacgca
     4801 actgaaatgc cgcatgatgc cgtatctgta tcgtgaagct gcgcgtgcga acgcgcgggg
     4861 tacgccgatg atgcgggcca tgatgatgga gttcccggac gatccggctt gtgattacct
     4921 tgaccgtcaa tacatgttag gcaacaacgt gatggttgcg ccggtgttca ctgaagcggg
     4981 cgatgtgcag ttctacctgc cggaaggtcg ctggacacac ctgtggcaca acgatgaact
     5041 cgacggtagt cgctggcata aacagcagca cggcttcctg agtctgcccg tttatgtgcg
     5101 tgataacact ctactggcgc tgggcaacaa cgatcaacgt cccgattacg tgtggcacga
     5161 aggcacggca ttccacctct ttaatctgca agacgggcat gaagccgtct gtgaagtgcc
     5221 cgctgctgac ggatcggtga tctttacttt aaaagcagca cgtactggca acacgattac
     5281 tgtgactggt gcgggcgagg cgaagaactg gacactgtgc ctgcgcaatg ttgtgaaagt
     5341 aaatggtctg caagacggtt cgcaggctga aagtgagcag gggctggtgg tgaagcctca
     5401 agggaatgcg ctgacaatta cgttgtaatc tattgatgga atgccgggta cgtctgagtc
     5461 gtacccggcg agattgatgt ttactgcacc agctctgccg acgtaacttc accgctggaa
     5521 acgatgccag cattcatggt ttgagtcact tgctgctttg cggcgttcac ggcatgcaat
     5581 tgcccgctaa ccgtaggttt cagcggggca ttggcctgaa tatctccact ggcggtaagc
     5641 tgaatattgc cgtcgccagt caacggtaat tcaggccatc cccattgttg caaaatattc
     5701 accggcacac cgcgtccatt caggctgata tgggtctgac gttgtggcgt ttgtgaaaca
     5761 ctggcagtgg cttccagaat gcctttttca gtaaatgcac tcagttcgct gatattcacc
     5821 gtgctgctgt tggcggtcag cgccagcgac gggcgacgaa catcaacacg attgaatgtc
     5881 gcggcggcgg cattcagatt cgccgagcca ctccagacgc cccatttatg atcggtaacc
     5941 agcgtcaggt tggcaccgta accatcgagc gcggtgagct gccacgggaa gtcagggtcg
     6001 atatcaatga tcagattgcg gctggcgcta aatctcttca gttgcaggct gtttaaccaa
     6061 ccgggtgtcg tttccatcca caactgttgc cagtttttcg gcaaggtata ttccagcccg
     6121 gcaattgcca catcatcaag gatcaacgtt ttcccgtcac gcagccagtt ccctgacgtt
     6181 ctgaccatac ccccttccca gcggctggtg aactggcgca gcgctacgcc ctgcggggaa
     6241 aattccgcgt ttataatcgg gtcaaataaa tgcagcgaac cataaatgaa ctcgctagcg
     6301 ttcatcgaca gtttgccttc ctgtgtctgc cagtcatctt tactgaaggt catgttgcgc
     6361 aagctgagat cgaggtcggt caccgcccag tccggacctt gcaaacgagc atcgatcact
     6421 tccaggcgac caatctgcaa cgacgggaca gagcgtaatg gcgcaaagaa gtcggttagc
     6481 gatttttcgc tttgtagacg tatatccgcc atgcgcaggt tttccacttg ccagctgccg
     6541 tcggcgttac gctgcgcgtt tccggttaat gtcccgcggg cgatgtcggc acccaggtta
     6601 gtcagcgtaa cgcgatcgtt atcaatactg ccttcaatca gtacattggt ggcaggaaca
     6661 tcattgagcg aaagcgatcc ggcactaaac tgaatctgcg ccttcgtacc cagcacttta
     6721 ccggcttttg gtgaccacgg aactacgccg ccatttatcc gctgcgcgct cagtttccat
     6781 tcgctattcg ggctattaaa cgccatatca cgcagttgca gacgatcggc tttgaacggt
     6841 agcggcgcgg tctggtcggt gagattcagc gtcccgtttt ccagcaggat ggtatcgaca
     6901 tggcgtggtt cggtcagttg ccgactgctt agcgcaatgt cgacactttt tgccaccagg
     6961 gtcgcgggct ggccatcacg gccaaacgtg acgttctcca gcacgatatg agatggcgcg
     7021 gaaaaacggt gatccatcgc cccgaaggcc agatgatagt cgctattctc ggaaacccat
     7081 gcgctgatat gttctgctcc ccagcgggtt tgcagaagaa aatagaggcc agcgatcacc
     7141 actaacagag cgatgagaat gtagagaagc agcttcccaa taaatttcat ggtcttccat
     7201 cccgtgaaat gcacataagg gagttatgca cgatttacgc gcaatcctca aggcgggaat
     7261 ggtgaaagag gtgagactgc ggcggtaatt atcttaccgc cgccagtgaa ttactgtttt
     7321 tctggtggga aaatcagatt cagcacgatg gcagtaatac cgcccgcggc gatcccggag
     7381 gagagcaggt ttttcagcca ttcaggggca aactgcaaaa tcagcggctg ctgagacacg
     7441 cccagaccaa ccgccagcga cagcgcgata atcagaatcg cccgacggtt cagcggttca
     7501 cgagaaacga tacgcacacc ggaggcggcg atagtgccaa acattacaag cgttgcgccg
     7561 cccagaaccg gttctggaat gtgttgtaca aaaccgctca ctgccgggaa cagacccagc
     7621 acgatcaaca tcagcgcgac gacaaaaccg acatagcggc tggcaacacc agtcaactgg
     7681 atcactccgt tgttttgccc gaagcaggag ttcgggaagg tgttaaacac cgccgaaaca
     7741 aacgagttca ggccgtttgc cagcacgccg cctttcaggc gtttcatgta cagcggaccg
     7801 gacactggct gttcggaaac gtcagaggtc gccgtgatat cgccaatggt ttccagcgaa
     7861 gtgatcataa agaccagcat cagcggcagc agcagactcc attcaatgcc aagaccgtaa
     7921 tagagcggcg ttggcaccat aatcagttct tgcgtcatcg gttcgttgct ttctggcaac
     7981 atgcccataa accacgccag cgcatatccg gccgccatcg caatcaccag tgaggccacg
     8041 cgtaagtaag ggttacgttg acggttaagc aggataatta aggctaagac cacgcctgcc
     8101 agcagcagat ttttcggtgc gccgaaggtg ttatcgctca tggctgcgta accgccgcca
     8161 atggacgtta acccaacctg aattagcgac aggccgataa tcatcaccac aacgccagaa
     8221 accagcggcg taataatgcg gcgcgccaga tgcagaacgc gggagatcac catctcggtg
     8281 caacttgcca gcatcaacgt gccgaacaaa gccgccatca tggtaggaac atcagcacca
     8341 ccggttttca gcgcggtacc gcccataatc agcggggcaa caaagttgaa gctggtgccc
     8401 tgaatagaca acagcccgga gccaaccgga ccccaggcct taatttgaat aatcgatgcc
     8461 acaccggagg caaacagcga catactaata atgtgttgcg tgtcttgtgc cggtaaaccc
     8521 agcgcctggc agattaatag cgctggcgtg atcaccgcaa cgaacatcgc cagcagatgc
     8581 tgacaggcgg caaacagggt ttgaggaagc ggcggacgat cttcaagacg gtaaatcagt
     8641 tcgctgtttt gagtctgcgc aaccggttgc gcattttctg actcgagggt ggaaacagac
     8701 atcgaaaaca atcccgtggt agcaaagcgg gcattttagc tgaccgaaag agaaaagcaa
     8761 acgtttgcca catcctcatc taaatattta aatgagataa atgcactttt ttatataact
     8821 tttggtattt ttctgcttaa aatccatgcc atttgttcat taacagctga gaatttgacc
     8881 caggaaagca taactcgata cccgtcatag ttcatgaagt tgccactgcg cgatgaagta
     8941 tgacgagtat gaaagagtga tgcggataca aaggagtaac tatgtttcat ctcgatactt
     9001 tagcaacgct tgttgccgca acgctgacgt tgctgctcgg gcgtaagttg gtccattccg
     9061 tctccttttt gaagaaatac accataccgg aacctgttgc gggtggtttg ttggtggcgc
     9121 tggcgctact agtactgaaa aaaagcatgg gctgggaagt caactttgat atgtccctgc
     9181 gcgatccgtt aatgctggct ttcttcgcca ccattggcct gaacgccaac attgccagtt
     9241 tgcgtgccgg tgggcgtgtg gttggcatct tcttgattgt ggttgttggt ctgttggtga
     9301 tgcaaaatgc cattggcatt ggtatggcta gcctgttagg gcttgatccg ctgatggggc
     9361 tgttggccgg ttctattacg ctttccggcg gtcacggtac gggcgctgcg tggagtaaat
     9421 tgttcattga acgttatggc ttcaccaatg cgacggaagt ggcgatggcc tgtgcaacgt
     9481 tcggtctggt gctgggcggc ttgattggcg gtccggtggc gcgctatctg gtgaaacact
     9541 ccaccacgcc gaacggtatt ccggatgacc aggaagtccc gacggcgttt gaaaagccgg
     9601 atgtgggacg catgatcacc tcgctggtgc tgattgaaac tatcgcgctg attgctatct
     9661 gcctgacggt ggggaaaatt gttgcgcaac ttttggctgg cactgctttt gaactgccga
     9721 ccttcgtctg tgtactgttt gttggcgtga ttctgagcaa cggtctgtca atgatgggct
     9781 tttaccgcgt ctttgagcgt gcggtatccg tgctgggtaa cgtaagcctg tcattgttcc
     9841 tggcgatggc gctgatgggg ctgaaactgt gggagctggc ttcgctggcg ctgccgatgc
     9901 tggcgattct ggtggtacag accatcttca tggcgttgta tgccatcttc gttacctggc
     9961 gcatgatggg caaaaactac gatgcggcag tgctggctgc gggtcactgt ggttttggcc
    10021 tcggtgcaac gccaacggca atcgccaaca tgcaggcgat cactgaacgc tttggcccgt
    10081 cgcacatggc gtttttggtg gtgccgatgg tcggtgcgtt ctttatcgat atcgtcaatg
    10141 cgctggtgat taagctgtat ttaatgttgc cgatttttgc cggttaaaaa tgaaaaagcg
    10201 tcggaaggaa ccggaaacgc tacgtgaaca ctgtcgccat atttttggtg atgagccgcc
    10261 agtgctctgt gtatgggaaa cggaatttga ttacgcagat gccgaactca aggcgctggc
    10321 ggctaaagag tggcagcaga tcagcgaatg ggatttgagt gcttactatg tactcaatct
    10381 cgtttataac gaaccgatgc aaatagagtt gtttcgctat ctgtttccgc tctgcctggc
    10441 gcaatggcat gaaaccgttt tagctggcgg ttatggtgat cacttcgaag aatccttaat
    10501 gaaggcattg tgtcggccct atctctggca ggagatgatg aatgcttccc aacgccagca
    10561 ggtacggcaa tttttactgg acaccgcgtt gcagcgtatg gataacgagc gtggtttcaa
    10621 taacgttctt tgctggctgg cggtgttcaa tactctgggt ggggccgcgc cgttaattcg
    10681 ttctctctgg tcgcgctggt gggcgctgga tacgccagga aaagcggtgt gtgctattca
    10741 gtatgccgcg catctaattt atcccataga agccaatccg ttatggtcgc aagaatggat
    10801 aggctggggg catccattag gccataaaga tggctggtcc tctgataatc gcgcttttct
    10861 tcgccaaatg ttaacacctg aaatgattgt ggctggcgta caggcagcgg ctgaaattct
    10921 gcgtggtgaa ccggaaggcg caatggcggc gcgaattgca caggatgcgt atgaggcaat
    10981 ggatatcctc actatccaga ttgaagattt actgcgtgac ttgtcttgtg atgagtctgg
    11041 tcatgcgttg gagtaacgtt ccgtctccgg catccagcgt tctatcaggg cttttgcttg
    11101 ttgtgggtaa cgttcgtgaa tatgtcgcgc caggcgctga acttccggga tcatcgcctg
    11161 atcgcgcagt aaatccgcca ctttaaactc agcattaccc gtctgacgcg tacctaacag
    11221 ttcacccggt ccgcgaatct ctaagtcttt ctgtgcaatc acaaaaccgt cgttgctgtc
    11281 gcgcagcact tgcagccgaa tttgcgcggt tttggagagc ggcgttttgt agagcagcac
    11341 gcagtgagaa gccaccgcac cacgacctac acgcccgcga agctggtgta actgcgccag
    11401 acccagacgc tccgggtttt cgataatcat cagactggcg ttaggcacat caacgccgac
    11461 ttcaataacg gttgtggcaa ccagcaggtg tagctcacct tgtttaaacg acgccatcac
    11521 cgcctgtttc tcggcaggtt tcatccgccc gtgtaccagg ccaacgttca actctggtag
    11581 cgccagtttc aactcttccc aggtagcttc cgccgcctgc gcttccagca attccgactc
    11641 ttcaatcaac gtacaaaccc agtatgcctg acgaccttca gttatgcagg cgtggtgcac
    11701 gcggtcaatg atgtcggtac ggcgggtatc aggaatagcg accgtagtca ctggcgtgcg
    11761 gcctggcggc agctcatcta tcaccgaggt atcgagatcg gcatacgcag tcattgccag
    11821 cgtgcggggg atcggcgtgg cggtcatgat caactgatgc ggatggaagc cctgctgctg
    11881 gcctttctcc cacaatgcca gacgctgatg cacgccaaaa cgatgctgtt cgtcgataat
    11941 caccagcgcc aggccgttaa actgcacctg ttcctggaag atggcgtgtg taccgacaat
    12001 catctgcacc tgaccgctgg cgatggcttc ctgctgtgcc agccgtgctt tacctttctg
    12061 cttaccggcg agccagccca cttcgatacc gagcggtgca aaccagttgc ggaagttatt
    12121 ggcgtgctgc tcggcgagta attcggttgg tgccatcaat gctacctgtt tgccgtgggc
    12181 aatcgcacgc aacgcggcga gggcggcgac cagcgtttta ccggaaccta catcgccctg
    12241 caccagacgc atcatcggca catccagcgc catatcgcgc tcgatctccg ccactacgcg
    12301 tgcctgtgcg cccgttggct tgaacggtaa ggcggcgagg agtttatttt tcagcgtgtc
    12361 attggcgctc agcggctggg catgaaaacg ctgtgctccg gcacgtaagg ctaacatgct
    12421 gaggttgtgc gccagcagtt cttccagaat cagacgacgt tgcgccggat gctgcccggt
    12481 ttccagatcg ctaagctgta gcgtcggtgg cgggcggtgc aaagtgcgca acgcttccgg
    12541 tagcgtcatc attccttgtg acagttccgg cggcaggagt tcttcaatgg cgcaggtgtc
    12601 gagcagatcc agcgcctggt cggttaattt acgcagcgtg gcctgcttta cgccttccgt
    12661 tgttggataa accggcgtga gcgtttcctg taattctggc gtgctgagat cgccctgcac
    12721 gcggtattcc gggtggatca tctccgcacc atatttaccg cgctttgctt cgccataagc
    12781 cagtacacgg cggcccgccg ccaggctatt tttcattgcc gcgctgaaat tgaaaaagcg
    12841 catggtgagg atgccggaac cgtcgctgat ctggcaggtc atcatccgcc gaccgccgaa
    12901 ggagatattg cagttcagca cttcgccttc caccgtggca taaacgcccg gcagtagttc
    12961 tccgatgggg tagagatggg tgcgatcttc gtagcgcagg ggaaggtgta agagtagatc
    13021 ctgcacggta tgcaggttga ttttcgccag tttgttacta agtgctgcgc caacgcccgt
    13081 tagggaactg agtgggacag catctaacag gcgacctttc atggcactta ccctgcagcc
    13141 tgcatagtag cccaccagtc ggcatcagct tcgatctcgc cttgctgatt gacgtgggga
    13201 taaggcaggc ctttgcgttt tgcgactttc gccagcaccg gatagccgcc ttcaaacaac
    13261 aggcgttgtt gctctgcttc cggcaacatg ctgttttcac gcaggtacat gcctgcattt
    13321 tgccgctgac gctgggcttc gtaaagaatg agggctgagg caacggacac attcagcgac
    13381 tgcaccatgc cgatcatcgg aatgatgatg tcctgatccg ccagggccaa tgcttcctgc
    13441 gtgatgcccg ttttctcctg tcccatcaaa atgcaggtcg ggcgagtgta atcaatttcg
    13501 cggaaatcga cagcgttatc agaaagatgg gttgccagaa tctgcatgcc ctggcctttg
    13561 agatgagcga cggcatcgcc aatggtgcgg tgtgttttca cctgtaccca gctgttacta
    13621 cccgccgctg ccgaagccat ggtgcgcatg cggctaccag gccagacggc gtgaacttca
    13681 tgtacgccaa cggcatctgc ggtacgaata atcgcagaaa cgttatgagg tttgtggacc
    13741 tgctccatgc agacggtcag atcaggctgc cgcctggcga gcatttcgca gatgcgtgca
    13801 taacgtgttg ggttcataaa acattaattt cggtttcggg tgactttaat cacgtctggc
    13861 atcacgcgga ttttgcgcat gatattcgcc agatgcacac ggtcacgagc ggtcagacga
    13921 ataaaggcgc tgtagacgcg accatctttc tcttccgtat tcaaactttg aatattcgag
    13981 gtcgtggtgt taattgccgc cgtcaggttt gccagcgcac cctgatgatt gaacatctcc
    14041 accttgattt cggtgatgaa ctcctgcgcc gtctctttat cccattccac agccataaac
    14101 ttctctggct ctttctggta gccacggatg ttacggcagg attcatggtg gatcaccaga
    14161 cctttaccgg ggctgacgtg ggcgataatc gggtcgccag gaatggggcg gcagcatttc
    14221 gcaaaggtga tcagcacgcc atcagcgcct ttgatcggca gatgcccgtg gctttgagtc
    14281 gccggtggaa tggaggcgtc cccatgttgt agatttttcg cgaccaccac gctcattgcg
    14341 ttaccaaggc cgatttctgc cagcagatcg tcaagcgttg ccagcttcat gcgatccagc
    14401 tcgcgctgaa tattttcctg cgggatttca ttgagcttac ggctaccacc caaagcatgg
    14461 ttgagcagac gacggcccag gcttacagaa tcatcacgct tgaggttttt cagcaactga
    14521 cgaattttgg cgcgcgcttt cgagctaacg acaaagttca gccaagcggc attcgggcga
    14581 gcgcccggag cggtaatgat ttcaacggtc tgaccgctag taagcggctg cgacagcggg
    14641 taaggctggc ggtcgacgcg tgcgcccacg caggcatggc cgatatcggt atgcactgca
    14701 taagcgaagt cgacgggcgt tgcaccggca ggcagctcga caatgcgccc ttccggtgtg
    14761 aaaacgtaaa tctcatccgg gaagagatcg gatttaacgc tctcgataaa ttcaaacgaa
    14821 ctaccggcgc tctgttgcag ctccagcagg ctttgcatcc agcgctgggc gcggatttgc
    14881 gcggtggtgc tggtttcgcc gtgctcttta taagcccagt gcgcggcaac acccatctcc
    14941 gccatctgat ccatatcttc ggtacggatc tggacctcaa ccggcacacc gtgcgggccg
    15001 atcatcgagg tgtgcaaaga ctgatagccg ttcgcttttg gaatggcgat gtagtctttc
    15061 acgcgacccg gacgcggctt gtacaggcta tgcatttgcc cgagcacgcg ataacaggtg
    15121 tcggaatcat tgacgatcac gcggaaagcg tagatgtcca tgattgagtg aaaacgctgc
    15181 tctttgagca ccattttgca gtaaatcgaa taaagatgct tctcgcgacc actgacgcgg
    15241 cacggaattc ccgcttcctg caaacgccct tcgatttcag agaggatttt ttggatcatc
    15301 tctttacggt tgccgcgcgc ggctttcacc acttctttaa ttacgcggta acgattggga
    15361 tacagcgcct caaaacccag ctcttcgagt tcggttttaa tgtggtggat acctaaacgg
    15421 tgcgccagcg ggctgtaaat ttcgagagtt tcacgggcga tgcggcgacg tttgtccggg
    15481 cgaagtgagc ccagcgtgcg catgttgtgg gtacggtcgg caagtttgat gaggatgacg
    15541 cggatatcct gcaccattgc cataatcatc ttgcgaaagt tttcggcctg cgcctctttc
    15601 ttatcacgga acttgagttt atcaagtttc gacaccccct ctaccagctc ggcgacgctt
    15661 ttaccaaaaa gctgttccat atcctggtag gtggcgggag tatcttcaat cacgtcatgc
    15721 agcagcgccg ccatcagcgt ttcatagtcg agtttcatct cggccagaat gcaggcaacc
    15781 gctaccgggt gcgtgatata gggttcaccg cttgaacgtg tttgcccctc gtgagcatca
    15841 cgtgcaacga gatacgcctg ccgcagacgc ttgatttggt cttccggcag gtaggtttga
    15901 atcagttgat tcaggctttc aaacagatac aagggcgacc cgctttgtga ttaacgacga
    15961 ccttcagcaa tagcggtaac ggcttgtaat tcagcggctt cctgctcttg ctgttcctgg
    16021 cgttcgcgaa cgtcgaggat ctggttgttg atcagacctt cttcgatttc gcgcagcgcg
    16081 attacagtgg ttttatcgtt ttcttccggt accagcggat cctttccgcc tacctgcatc
    16141 tgacgagcgc gacgcgcggc gaccagtacc aggtcaaaac ggttaccaat tttctctaca
    16201 gcgtcctgaa cagttacgcg tgccatactt aaaaagctcc acaggtgaag aaatgactgg
    16261 gcatgatact gaaatcaggt tcagtctgcc aacaatttgc tgattaaagc gtcatgacgc
    16321 tgcttttggc ggctcatgcg cagacgttcg gcgcgaataa tggtcttcaa atcggtcaac
    16381 gcggtatcga agtcatcatt cacaatcaga taatcatatt cggcgtaatg gctcatttct
    16441 gcaacagctt gcgccatacg ctttgcaatg acctcttcgc tgtcctgacc gcgaccgcgt
    16501 agacggcggt ccagttcaat tttggacggc ggtaaaataa agatactccg cgcgtgcggc
    16561 atcttctggc gaatttgctg cgcgccctgc cagtcgatat cgagaaaaac atcgacaccg
    16621 gtcgccagta cttgctcaat ggcctcacgc gaagtgccat agtaattacc aaaaacttct
    16681 gcgtgttcga ggaacgcatc tctgctaatc atttctttaa attcatcatg attaacaaag
    16741 aaataatgtt caccgtggac ttcaccagga cgcggttggc gtgtggtgtg tgaaacagaa
    16801 acctgggtgt catacaacgg ttgggttttt aataaagcct gaatcaggct ggatttaccc
    16861 gcgccactgg gggcagaaac aatataaagc gtgccttgag ccatgagtat ctttcgtatg
    16921 tgattagcga aataaagcct acatacgagc ttattataca cggcgcggcc atgtgacgta
    16981 gcccttgctc acactttttg cgcaaagttg ttgcgttttg cgtagctgtt tccgcatttg
    17041 cctgtgattt actgcaataa agacgattct caggaagcgt cctcgcagac tccgtttttg
    17101 ccactcggct ttgccttcac cacgttgtat atctgccagc ctgactacag cggaggtgaa
    17161 gatgaaagta tggatggcga tattaataag tatcttgtgc tggcaatcat ctgtgtgggc
    17221 ggtctgtccg gcctggtcgc cagccagagc acaggaagaa atttcccgcc tgcaacagca
    17281 aataaaacag tgggacgatg actactggaa ggaaggaaaa agtgaggtgg aagacggtgt
    17341 ttacgatcag ttaagcgccc gtcttacgca gtggcaacgc tgctttgtga gcgagccccg
    17401 cgacgtgatg atgccgcctt taaatggtgc ggttatgcat ccggttgccc atacgggtgt
    17461 acgcaaaatg gcggataaaa acgcattaag tctgtggatg cgagaacgta gcgatctttg
    17521 ggtgcagcca aaagttgatg gcgttgcggt aaccctggtt tatcgggacg ggaaactgaa
    17581 caaagcaatc agtcgcggta atggcctgaa aggcgaggac tggacgcaga aagttagctt
    17641 aatttccgct gtgccgcaaa ccgttagcgg gcctttagcc aacagtacgc ttcaggggga
    17701 aatatttctc cagcgcgagg ggcatatcca acaacaaatg gggggaataa atgcccgcgc
    17761 aaaagttgct ggcctgatga tgcgccagga cgatagcgac acgctgaatt ctctgggcgt
    17821 ttttgtctgg gcatggccgg atggaccgca gttaatgacc gatcgtttaa aggagctggc
    17881 taccgcaggt tttactctaa cgcagaggta tacccgtgcg gttaaaaatg ctgatgaggt
    17941 tgcgcgcgta cgcaatgagt ggtggaaagc gaaattaccc ttcgtcaccg atggcgtggt
    18001 tgtgcgaggg gcgaaagaac ccgaatcccg ccattggcta ccaggccagg cagaatggct
    18061 ggtggcctgg aaatatcaac ctgtagctca ggttgccgaa gtgaaggcga ttcagtttgc
    18121 ggtgggtaag agcggtaaaa tatcggtggt tgcgtcactc gcacctgtca tgctggatga
    18181 taaaaaagtc cagcgggtga atattggttc cgtcaggcgc tggcaggagt gggatattgc
    18241 gcctggtgat cagatcctcg tcagccttgc cggtcagggt attcctcgca ttgatgatgt
    18301 ggtctggcgc ggtgcagaac gtacaaaacc gacaccacca gaaaaccgct ttaactcgtt
    18361 gacctgctac tttgcttctg atgtttgtca ggaacagttc atttcacgct tagtctggct
    18421 ggggtcaaaa caggttctgg ggctggatgg tattggtgag gccggctggc gcgcgctgca
    18481 tcagactcat cgctttgagc atatcttttc ctggctttta ttaacgccag agcaattaca
    18541 gaacacgccg gggatcgcga aaagtaaaag tgcgcagcta tggcatcggt ttaatctggc
    18601 tcgtaagcag ccttttactc gctgggtgat ggcaatggga ataccgctaa cccgggcggc
    18661 gcttaatgcc agtgatgaac ggtcctggtc gcaactttta tttagcacgg agcagttctg
    18721 gcagcagctg ccggggactg gatcggggcg cgccagacag gttattgaat ggaaggaaaa
    18781 tgcgcaaatc aagaagctgg gcagttggct ggctgcccag cagatcacag gttttgaacc
    18841 ttagtgtcct tcgtggctgt agtaaaagac cggtaatccc agcttaaggc gtagcgccag
    18901 taagcgggca aaaaagccga ataccagggt ggagataatt accacatcat ggttagaaac
    18961 atagtgttgc agcgcgatgt acagcacggc ggaggcaaat gagacaccgg catacaactc
    19021 tttctggaat accagtggga tacgtttaca gaacatatcg cgcaaaacgc cgccaaacac
    19081 gccggtggtt accgccgcga caacggcaat aattgggccg tgccccatat ccagtgcaac
    19141 ctgtgcgccg atgatagaaa agaccaccag tccaagcgca tcgagcacca gaaacacttt
    19201 gcgcaggtaa ggcattacag gagcaacgat agtggtgagt actgcggcgg tagcgacgat
    19261 aatcacatat tccgggtgtt tgacccagcc gagcggatag tggcccagca gaatatcgcg
    19321 cactgaccct ccgccaattg cggtggcggt cgcaataata attacgccaa atgtatccat
    19381 gcggcgtcgc ccggccgcca gagcccccgt catggcttcg gcagtaatgc caaccaaata
    19441 caaaatgtgt aacagcatgg ttctctccct aaaaatctgt ctggcagggt agcgatttgt
    19501 gcgtattgtc acgattgaga ttttttaagg tgagaggaaa ttgataactt gtagttatgt
    19561 gaaatcgcta ttttctgtag cgtagatgca ttcttctgac ttcttgtctg agaaaatgca
    19621 taagaaaaaa taggttaaaa ttgaggggtg gaaaagaaag gtgcaatgtt tagccattgt
    19681 gttcaccgca cctggacttt ggcttacgca tcgggtatct caatcaacaa aaagagtttt
    19741 tagtttctgc tatttcttct ctgtaatttt tacactgttt tccaactgct tgatgctgac
    19801 ctgcggggtc ggcaactcct caggcatggt tccgccaagt tcctgaatgg tttgcctcac
    19861 cttgctaccc acgtcaaagt gtgtggtatt agcttgctgt tttgaattca cgccatcccg
    19921 cttgagtttt tcttctgttt gggtagctcg aaagagatta gccgccagtt ctgttgaacc
    19981 catatgatcc aggatcttct gactcttttt cagccccttc cgctgatgga tggctttctg
    20041 atctaatcca ccatacagcc cccggtaacc atgattctga aatatggcga aatcgatagc
    20101 tgtcgctacc ccagcttgct gcgctgcctc aaccaattgt ttgttatgtt ctttcaattc
    20161 atttctcaga aaaagacgct tttcgtcttc acgaagttgc ctgaaagctt catcgtcagc
    20221 cagctcctgc cgtcgggtct ggatagcaaa ataagtttgc cctgccgcaa taaccggttt
    20281 cgcagggtcg ccgttttgta ctaccaggta acaggcatag cgggagagat gaacatcttc
    20341 aagttctcgt tgagcccctg aacctaatac aaccattttg gtggtttcaa cgaaatggtc
    20401 agatgcagct tgattgctgg cttcgcacgc ttgagtcgcg cgggccagta ccttttgaaa
    20461 attgcgccag tcccgataat ccagtaacgg tgccaattct ctagctgacc aaaattcttg
    20521 cccttcagtg ccataatgcc gtatttcttc aaaaggttga tgatgttcgt tcattgccca
    20581 ctccatgtga tatcgattgc aaagaaagta aatctgtaac tgtatttata tacagttgtg
    20641 agtatctcac gttctcagtg tgctgggcaa aaaaacatcg caaaatctgg agcaggcacg
    20701 caaaacagaa ttgaagttgc ggggaagaaa aatgccgctc tgtttgagtg ggcggcattt
    20761 gtgaaggctg aaaagtaaga attagtgtta cgagttactc aatattctga atctgctcgc
    20821 gcatctgctc aatcaacact ttcagctcaa tcgcggagtt tgtcacttcg gcattgatag
    20881 acttcgacgc aagagtgttc gactcgcggt tgaattcctg catcataaag tccagacgac
    20941 gaccaacggc ttcttttttc ttcagaatgt tgtaggtctc tttgacatgc gcttccaggc
    21001 ggtccagttc ttcggcaacg tcaattcgtt gtgccagcag aaccaactcc tgctccaggc
    21061 ggttgttttc cagttgcact tgagcatctt ccagcttcgc gaccagacgc tcacgctgcc
    21121 attgcaggat ttccggcata tgggcgcgga ctttgaccac ttcggcggtg acgccttcca
    21181 gacgctgctc gatcaatgct ttcagcgcct gaccttcggt ttcacgtgcg acgataaagt
    21241 cgtccagggt accatccaac gccgcgagaa tttcagcggc aatcgcgtcg agatcctgct
    21301 cctgggctgc cattacgccc ggccagcgca gaatatcaac cgggttgatt tccccttcgt
    21361 cactctgcat ttttacccag ttcgcggcag ttaccagctg tttagccagt ttttcgttga
    21421 ggatcagctc accttgcgcg ctaacatctg gctcatagcg cagggtacat tccactttac
    21481 cgcgcgtcag gcgagaacga atacgctcgc gaacgacagg ttcaaggcta cggaactgct
    21541 ccggcagacg aaagtaagtt tccagataac gctggtttac cgagcgcatt tcccaggttg
    21601 cgctccccca ttcacccttg atttcacgcc gggcgtaggc ggtcatactg cggatcatag
    21661 acgttcctgt ttataaaagg agaggtggaa ggattatagc catcgatgcc ttgtcaggat
    21721 aggaataacc gccggaagtc cgtataatgc gcagccacat ttgtttcaag ccggagattt
    21781 caatatgcgt ccagcaggcc gtagcaataa tcaggtgcgt cccgttaccc tgactcgtaa
    21841 ctatacaaaa catgcagaag gctcggtgct ggtcgaattt ggcgatacca aagtgttgtg
    21901 taccgcctct attgaagaag gcgtgccgcg cttcctgaaa ggtcagggcc agggctggat
    21961 caccgcagag tacggcatgc tgccacgttc tacccacacc cgtaacgctc gtgaagcggc
    22021 gaaaggtaag cagggtggac gcacaatgga aatccagcgt ctgatcgccc gtgctcttcg
    22081 cgcggcagta gatttgaaag cgctgggtga gttcaccatt acgctggact gcgacgtgct
    22141 tcaggctgat ggtggcacgc gtaccgcgtc gattacgggt gcctgcgtgg cgctggcaga
    22201 tgcgctacag aagctggtgg aaaacggcaa gctgaaaacc aacccgatga aagggatggt
    22261 agccgcagtt tctgtcggta ttgtaaacgg tgaagcaatt tgcgatctgg aatacgttga
    22321 agactcagcc gcagagaccg acatgaacgt agtgatgacc gaagacgggc gcatcattga
    22381 agtgcagggg acggcagaag gcgagccgtt cacccatgaa gagctactca ccttgttggc
    22441 gctggcccga gggggaatcg aatccattgt agcgacgcag aaggcggcgc tggcaaactg
    22501 atttttaagg cgactgatga gtcgcctttt ttttgtctgt agaaaagtaa gatgaggagc
    22561 gaaggcatga aaccatatca gcgccagttt attgaatttg cgcttggcaa gcaggtgtta
    22621 aagtttggcg agtttacgtt gaagtccggg cgcaaaagcc cctatttctt caacgccggg
    22681 ctgtttaata ccgggcgcga tctggcactg ttaggccgtt tttacgctga agcgttggtg
    22741 gattccggcc ttgagttcga tctgctgttt ggccctgctt acaaagggat cccgattgcc
    22801 accactaccg ccgtggcgct ggcggagcat catgaccttg acctgccgta ctgctttaac
    22861 cgcaaagagg caaaagacca cggtgaaggc ggcaatctgg ttggtagcgc gttacaagga
    22921 cgcgtaatgc tggtagatga tgtgatcacc gccggaacgg cgattcgcga atcgatggag
    22981 attattcagg ctaatggcgc gacgcttgct ggcgtgttga tttcgcttga tcgtcaggaa
    23041 cgggggcgcg gcgagatttc ggcgattcag gaagttgagc gtgattacaa ctgcaaagtg
    23101 atctctatca tcaccctgaa agacctgatt gcttacctgg aagagaagcc ggaaatggcg
    23161 gaacatctgg cggcggttaa ggcctatcgc gaagagtttg gcgtttaaag aaactcgccg
    23221 gatgaaaagt catccggcgt catattactg caactgtgcc gcaattagcg gccagcgggc
    23281 gtcaaaatca tccgtcgggc ggtatttaaa ttcgctgcgg acaaaacgtg acagcatacc
    23341 ttcacagaag gccaggatct ggcttgccag cagggtttca tcggtggtgt aaccttcacc
    23401 ctcacgcatt ctcttttcac gcaatacctg gcgcagctgc gcttcaatac gctcgaacag
    23461 ctggttgatg cgcccttgca ggcgatcctg ttcaaacatt agcgcatgac cagtgaggat
    23521 gcgggtcagg ccaggattac gctcaccaaa accgagaagc agcaacacaa tcagacgcag
    23581 gcgcgctgtg gtgtctttct catctttcag aatcaggttg atgcgagtaa tcaggctatc
    23641 ttcgataaac tcaatcaggc tatcgaacat gcgggtctta ctggggaagt ggcgatacag
    23701 tgccgcttcg gaaacgccga cagaggcggc cagttttgcc gtcgtgatac gttggcttcc
    23761 atcgctggat tccagcatca gcgccagaga ctgaagtatt tcctcgcgac ggttcctttt
    23821 cgcagtttgt ttttctgcca tgttacaaaa tacccctgaa aataagcact tgccaggcgg
    23881 cacccacgct atgaccgcaa acgaaatgtt tgcggctatg ttatgacgtt attcggatgc
    23941 gtatgtgtta ctgacgacca gagtgaccaa agccgccttc accgcggtcg gtggcgtcga
    24001 aatcttccac cagattaaat tcagcctgta ctaccggaac aaaaatcatc tgggcgatgc
    24061 gttcgccagg ttgaatggtg aagctgtcct gaccacggtt ccacacggaa atcatcaact
    24121 ggccctgata gtcagaatcg atcaatccta ccaggttacc aagcacgata ccgtgcttat
    24181 gtcccaatcc ggagcgcggc agcatcattg ccgccagtga aggatcggca atatgaatcg
    24241 ccagcccggt cggaaccagc gtagtgtcac ccggagccag ttctacggcg tcgtcgagac
    24301 aggcacgcag gtcaagtccg gcagagccag aggtggcata agtcgggagc ggaaattcct
    24361 tcccaacgcg cgggtccaga atcttaacgt cgattttttt catcataacg ggtcacgatc
    24421 tcgtcgagta ataattggcc aaggagctct ttgcgctcaa gcggtaagac tttatctccg
    24481 tcctgccaga aaaggtgtaa tgcgttgttg tcgctgttaa atccttgagt tggctgggaa
    24541 acatcgttcg cgcagatcag atcaaggttt ttacggatac gtttttgccg ggcgtattct
    24601 tccacattat ttgtttcggc ggcaaatcca acgacgtagg gtcgatggtc ttttagtgcg
    24661 gcaacgcctg cgacgatatc ggggttttta accattttta ttgttaattc atcaccctgc
    24721 gtggcctgct ttttgatttt ctctggggcc acggtagctg cgcgataatc cgccacggcg
    24781 gcgcagccga taaaaatatt ttgctgctgt acagaagcat tcacggcggc ttccatttcc
    24841 agcgcagtca tcacatcaac acgtttaaca aacggtggcg tcggtagtga aaccggacct
    24901 gataccagcg tgacgttcgc gccacgacgg gcagcggcgg cggcgatagc aaaacccatc
    24961 ttgccggagc tgtgattaga gatataacgc accggatcga gcggttcacg cgtcggaccg
    25021 gcggtaatca taatgttcag atgtttcagg tcgttgacgg gcgaaaaatg cgccaccgcc
    25081 atatccacaa tggttaacgg atcgagcatt cgcccaggac cgatatcacc acaagcctga
    25141 ctgccactgt ctggccccca gatgagcaaa ccacgggaag caagcacctc taaattatgc
    25201 tgcgtggcag cggcacggta catctgctgg ttcatggcgg ggagcacggc tacaggcgca
    25261 ggtgtagcca gacaaatcgt cgataccagg tcattcgcca ttccggcagc aacacgggca
    25321 atcaaatctg ccgtggcagg ggcgagaatc actaaatcag cccatttacc cagctcaata
    25381 tggcccatag cggcttcggc tgccgggtcc agcaaactgt cggaaacggg ataaccagaa
    25441 accgcctgca agctaagtgg ggtgataaag gcttttgccg cttcggtcat ggctacgcgg
    25501 acgtcggccc cgcgatcgcg caaacgacgc accagttcag gggttttata ggcagcaata
    25561 ccgccgctaa cgccgagaac gattttttta ccggccaggc tcatgatgat ttttcctgtt
    25621 gggtgacacc agaagttggc gattttatca caatactttt gttgtcgtgc cttcacctga
    25681 gaatcacttt gcgaggcgct ttccaggatt gaaaactggc cgtcgattta acggaacggc
    25741 tatgacagga tgcgagcacc acaaaggagg tgaaggtgaa aaacaatgca cagctgttga
    25801 tgccgcgcga aaaaatgctg aagtttggta ttagcgcctt aacggatgtc gagctgctgg
    25861 cgttatttct gcgtaccgga acgcgcggta aagatgtatt aaccctggca aaagagatgc
    25921 tggagaattt cggctctctt tatggcttgt taacctctga atatgaacaa tttagtggcg
    25981 ttcatggaat tggcgtagcg aaatttgccc agttaaaggg gattgctgaa ctggcgcggc
    26041 gttactacaa cgtgcggatg cgtgaagaaa gccctttact cagcccggag atgacgcggg
    26101 aatttttaca aagccagctc acgggtgagg agcgggagat ctttatggtg atctttctcg
    26161 actcacaaca ccgggttata acgcatagcc gtcttttttc cggcacgcta aaccatgttg
    26221 aagtccatcc tcgggaaatt atccgcgaag cgataaaaat aaacgcctcg gcgctgatcc
    26281 ttgcacataa tcacccttcg ggttgtgctg aacccagtaa agcggataaa ctcattactg
    26341 aacggataat aaagagttgt cagttcatgg atttacgcgt gctcgaccat atcgtgattg
    26401 ggcgtggaga gtatgtttct tttgccgaac gcggctggat ttaacccgct atgcgcgatc
    26461 cttcgggatc tttgtctgtt cgggacttga gcacatcgct gagtcagcgt atactacgcc
    26521 acctttgaga atctcgggtt tggcatttgg gcctggcaat cgagagttca catagaactg
    26581 cgatgaccgg gctgtaaagc ctgacgaggt gccaataccc catacgaagc tcgagctaat
    26641 ttgatttttg gagaatagac atgtcccgag tctgccaagt tactggcaag cgtccggtga
    26701 ccggtaacaa ccgttcccac gcactgaacg cgactaaacg ccgtttcctg ccgaacctgc
    26761 actctcaccg tttctgggtt gagagcgaga agcgttttgt caccctgcgc gtatctgcta
    26821 aaggtatgcg tgtaatcgat aaaaaaggca tcgatacagt tctggctgaa ctgcgtgccc
    26881 gtggcgaaaa gtactaagta cttagaggaa ataaatcatg gctaaaggta ttcgtgagaa
    26941 aatcaagctg gtttcttctg ctggtactgg tcacttctat accactacga agaacaaacg
    27001 tactaagccg gaaaaactgg aactgaaaaa attcgatcca gttgttcgtc agcacgtgat
    27061 ctacaaagaa gcgaaaatca aataattctc gctttgatgt aacaaaaaac ctcgctccgg
    27121 cggggttttt tgttatctgc ttgcccccat attgattgca tctgttcatt cctggagatg
    27181 ctatgcctga attacccgaa gttgaaacca gccgtcgcgg catagaaccg catctcgttg
    27241 gtgcaaccat tcttcatgca gtggtgcgca acggacgctt gcgctggccg gtttcagaag
    27301 agatctaccg tttaagcgac caaccagtgc ttagcgtgca gcggcgggct aaatatttgc
    27361 tgctggagct gcctgagggc tggattatca ttcatttggg gatgtctggc agcctgcgca
    27421 tccttccaga agaacttccc cctgaaaagc atgaccatgt ggatttggtg atgagcaacg
    27481 gtaaagtgct gcgctacacc gatccgcgcc gctttggtgc ctggctgtgg accaaagagc
    27541 tggaagggca taatgtgctg gcccatcttg gaccggagcc gctcagcgac gatttcaatg
    27601 gtgagtatct gcatcagaag tgcgcgaaga aaaaaacggc gattaaaccg tggctgatgg
    27661 ataacaagct ggtggtaggg gtagggaata tctatgccag cgaatcactg tttgcggcgg
    27721 ggatccatcc ggatcggctg gcgtcatcac tgtcgctggc agagtgtgaa ttgttagctc
    27781 gggtgattaa agcggtgttg ctgcgttcga ttgagcaggg cggtacaacg ctgaaagatt
    27841 ttctgcaaag tgatggtaaa ccgggctatt tcgcacagga attgcaggtt tacgggcgaa
    27901 aaggcgagcc gtgtcgggtg tgcggtacgc cgattgtggc gactaaacat gcgcagcggg
    27961 caacgtttta ttgtcggcag tgccagaagt aattcatgcg cgccggatgg cataccatcc
    28021 ggcataaacg ctacgctaac ttcgccatca gcgcctgatg gacattctcc ggcaggaaat
    28081 gggtgacatc gccctgatgg cgcgccacct ctttcaccaa cgatgaagag ataaacgacc
    28141 actctttcga cggcatcaga aacacacttt ccagttccgg cattaagtgg cgattcatat
    28201 gcgccagctg catttcatat tcaaaatccg ccaccgcacg caggccacga atcagcaccg
    28261 tagcgtgttg attacgggcg aagttcgcca tcaaatcact aaacccgacc acttccacgt
    28321 tccccagatg cgcggttgcc tgctgtgcca gcgccacacg ctcttccagg gtaaacatcg
    28381 gttttttact ggggctggcg gcaatcgcca gaataacgtg atcgaacatt tgcgtggcgc
    28441 gcgtcacgat atcgatatga ccattggtaa tgggatcgaa agtacccgga taaatcgccc
    28501 gtttttgcat aacaacctca atgcgttttc ggtggcaggt aaggttccag cagttgaagc
    28561 agacgctgta gcgcgccctg gttttgatac agtacttcaa cggcatgacg gccatagaaa
    28621 ctacggtaat cggcgtcggt gagtaaagag gacacctctt ttgcaagcgt agtggcatcg
    28681 gtaacggtaa tcagcccgct tgcctgctcc agccgcgcgc aaatgtcttt aaagttaaaa
    28741 gtatgcggcc ccatcaatac cggaatagcg tgtgcggcag cttccagcgg attatgccca
    28801 ccacgttcaa ccagtgaacc gccaacaaag gcgagatcgg caatgccgta cagtaacatc
    28861 aactcgccca tcgtatcgcc aaccacaacc tgcgtgctgg tggaggggac ttcccctgaa
    28921 gagcgtgtga tatagcttag tccagcctgg cggacaagat taatcgcatc cgggaagcgt
    28981 tctggatgac ggggtaccag gatgagcaat aaattcggga attgctgtaa caatgcctga
    29041 tgtgcggcga tcaccacact ctcttcgcct tcgtgagtgc tggtggcaat ccataccggg
    29101 cggtgtggtg cccactggcg gcgcagcgtc acggctttag cagccaactg cggcgttaca
    29161 gaaatatcga atttcaggct accggtaacg gtcacctgat tattttttgc gcccagcgcc
    29221 acaaaacgtg caccatcttc ttcattttgc gcagcaatca gcgtaatacg acgcagcaag
    29281 cgacggacga atttacccag tttggcataa cctgcggccg agcgggcaga gagtcgcgcg
    29341 ttagcgatca ccagcggaat tttacgtttg tgtagcgccg caatcaggtt aggccatagt
    29401 tcggtttcca taatcaacac cagtttgggg tcgactttat tcaggaaacg gttgagcgca
    29461 tcgggcaggt catacggcag ataaacgtgc tgaacatcct tcccgaaagc cgattgtacg
    29521 cgctccgaac cggttggcgt catggttgta acggtaatcg gtaaatcagg ataacgatgg
    29581 cgcagcgcgc gcaccaacgg gatcgccgcc agagtttcac cgacggagac ggagtgcagc
    29641 ataatgccgc ctggttttag cggatggcgg taaaaaccgt aacgttcacc ccagcgtttt
    29701 cgataggccg gagccttacg tccgcgcacc cagagccgta tccagatcag cggctgaata
    29761 aggtagagaa gggcggtgta aagcaattcg agcatagtaa atagctgact tatggatgtg
    29821 ctggggattc tatgtattta gctgtggctt taccattact tttcccgttt ttgacttaaa
    29881 tagcaattta cccgccaatt cttgctgaac atctatactt tttttgtatt tttatgcgat
    29941 tcattgattt ctgctgtacg tgcaggaagg aatcgcagag caacagtgaa agaatatggt
    30001 tgctaatgat tttatttgat tgatttcttt attgaaaatc aacaatgtgc ttaaaaatca
    30061 ttcgggtcgg attgccgcga aagaagtgat acactagcgc gtcaatatca gtgcggtatc
    30121 agtattcagg tagctgttga gcctggggcg gtagcgtgct ttttctgctt caccttaatc
    30181 ggataatctc aacaaaaaga gttacttgtg gataagccat ttcgaagaat tttgctcatt
    30241 aagatgcgtt ttcatgggga tatgttatta actactcccg tcattagttc gctgaaaaaa
    30301 aattaccctg acgcaaaaat cgatgtgctg ctttatcagg acaccatccc gatcctgtct
    30361 gaaaatccag agattaacgc gctctacggc ataaaaaata aaaaagcaaa agcctcagaa
    30421 aaaattgcca acttttttca tctcatcaag gtattacgtg ccaataagta tgaccttatc
    30481 gtcaatctca ccgatcaatg gatggttgct atactggttc gcttattaaa tgcccgtgtg
    30541 aaaatttccc aggattatca tcatcggcag tctgcttttt ggcgtaaaag tttcacccat
    30601 ttggtgccgt tgcagggtgg aaatgtggtg gaaagtaact tatccgtgct gaccccattg
    30661 ggagttgatt cgttggtgaa gcagacaacc atgagttacc cgcctgcaag ctggaaacgt
    30721 atgcgtcgcg aacttgatca cgctggtgtt ggacaaaatt atgtggttat ccaacctacg
    30781 gcgcggcaaa tcttcaaatg ctgggacaac gccaagtttt ccgctgtgat tgatgcctta
    30841 catgctcgtg gttatgaagt cgttctgacg tccggcccag ataaagacga tctggcctgc
    30901 gtcaatgaaa ttgcgcaggg atgccagacg ccaccagtaa cggcgctggc tggaaaggtg
    30961 accttcccgg aacttggtgc gttaatcgat catgcgcagc tgtttattgg cgttgattcc
    31021 gcaccggcgc atattgccgc tgcagttaat acgccgctga tatcgctgtt tggtgcgaca
    31081 gatcatattt tctggcgtcc ctggtcaaat aacatgattc aattctgggc gggagattac
    31141 cgggaaatgc caacgcgcga tcagcgtgac cgaaatgaga tgtatctttc ggttattccg
    31201 gcggcagatg tcattgctgc tgtcgataaa ttactgccct cctccacgac aggtacgtcg
    31261 ttatgatcgt tgctttttgt ttatataaat attttccctt tggcggtttg cagcgtgatt
    31321 ttatgcgtat tgctcagaca gtcgccgccc gaggtcatca tgttcgggtt tatacccagt
    31381 cgtgggaagg cgaatgccct gatgtatttg aactgatcaa agtgccggtt aaatcgcata
    31441 ccaatcacgg gcgcaatgcg gagtattttg cctgggtgca aaaacattta cgcgaacatc
    31501 ccgtcgataa agtcgttgga ttcaacaaaa tgccggggct ggacgtttat tatgccgctg
    31561 atgtttgtta tgccgagaaa gtagcgcagg aaaaaggctt tttctatcgc ctgacgtcac
    31621 gttatcgcca ttatgccgcc tttgagcggg caaccttcga acagggtaag ccgacacagc
    31681 tgctgatgct gacagataag caaatcgccg atttccagaa acattatcag actgaagcgg
    31741 agcgttttca tattctgcca ccggggattt atcctgatcg taaatatagc cagcagccag
    31801 ccaatagccg tgaaatcttc cgtaagaaga atggaataac cgaacaacaa tatttattgt
    31861 tgcaggtcgg ttcagacttc acgcgtaaag gtgtcgatcg ttccattgaa gcacttgctt
    31921 cgttaccgga ttcgctgcgc cacaacacat tgctatatgt tgttgggcag gataaaccgc
    31981 gaaaatttga ggcgctggca gaaaaacgcg gcgtgtgcag taatgttcac tttttctcgg
    32041 ggcgcaatga tgtctcggaa ttaatggcgg cggcggattt attactgcat cctgcctacc
    32101 aggaagcggc gggaattgtg ctgctggaag cgattactgc aggattaccg gtactaacaa
    32161 cagccgtttg tggctatgcg ccttatattg tcgaggctaa ttgcggcgag gctattgctg
    32221 agccattccg ccaggaaaca ttgaatgaga ttttacgcaa agcgttaacg caatcttcat
    32281 tgcgccaggc ctgggcggaa aatgcgcgat attatgctga tacacaagat ttatacagtc
    32341 tgccagagaa agcggcggat atcataacgg gtggtctgga tggttgaact taaagagccg
    32401 tttgccacgt tatggcgcgg caaagatcct tttgaggaag ttaaaacctt gcagggtgag
    32461 gtatttcgtg aactggaaac tcgccgtact ctgcgctttg aaatggcggg caaaagctat
    32521 tttctcaaat ggcatcgcgg cacgaccctg aaagagataa tcaaaaattt actctcattg
    32581 cggatgccag tattaggcgc agaccgcgaa tggaatgcga ttcatcgact gcgggatgtc
    32641 ggcgttgata ctatgcatgg ggtggcattt ggcgaaaaag gcatgaatcc gctgaccaga
    32701 acttcattta ttattaccga agatctgaca ccaaccataa gtctggaaga ttactgtgct
    32761 gactgggcga ctaaccctcc agatgttcgc gtaaagcgta tgcttattaa gcgtgtcgcg
    32821 acgatggtgc gcgatatgca tgctgcgggc attaaccacc gtgactgtta tatctgtcat
    32881 ttcctgctgc acttgccttt ttccggtaag gaagaggagt taaaaatttc ggtaattgac
    32941 ctgcaccggg cgcagcttcg cacgcgcgtt ccacgtcgtt ggcgggataa agatcttatt
    33001 gggctttatt tttcttcgat gaatatcggc ctgactcagc gggatatctg gcggtttatg
    33061 aaagtgtatt ttgccgcccc gcttaaagac attctcaagc aggaacaagg actgctgtcg
    33121 caagcagaag caaaagccac aaaaatcagg gaaagaacga ttcgaaaatc gttgtaaccc
    33181 gtaacttatt tttgccaaaa ttttggatac aga
//
LOCUS       DAZFJF010000041        32603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFJF010000041 DAZFJF010000000
VERSION     DAZFJF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.041"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(78..239)
                     /gene="rsmS"
                     /locus_tag="ACHKAU_002148"
     CDS             complement(78..239)
                     /gene="rsmS"
                     /locus_tag="ACHKAU_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO5531211.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(253..780)
                     /gene="priC"
                     /locus_tag="ACHKAU_002149"
     CDS             complement(253..780)
                     /gene="priC"
                     /locus_tag="ACHKAU_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO5531212.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            850..1227
                     /gene="ybaN"
                     /locus_tag="ACHKAU_002150"
     CDS             850..1227
                     /gene="ybaN"
                     /locus_tag="ACHKAU_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO5531213.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTMPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1380..1931
                     /gene="apt"
                     /locus_tag="ACHKAU_002151"
     CDS             1380..1931
                     /gene="apt"
                     /locus_tag="ACHKAU_002151"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO5531214.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            2060..3991
                     /gene="dnaX"
                     /locus_tag="ACHKAU_002152"
     CDS             2060..3991
                     /gene="dnaX"
                     /locus_tag="ACHKAU_002152"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO5531215.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3324..3388
                     /gene="dnaX"
                     /locus_tag="ACHKAU_002152"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4044..4373
                     /gene="ybaB"
                     /locus_tag="ACHKAU_002153"
     CDS             4044..4373
                     /gene="ybaB"
                     /locus_tag="ACHKAU_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO5531216.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4373..4978
                     /gene="recR"
                     /locus_tag="ACHKAU_002154"
     CDS             4373..4978
                     /gene="recR"
                     /locus_tag="ACHKAU_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO5531217.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5088..6962
                     /gene="htpG"
                     /locus_tag="ACHKAU_002155"
     CDS             5088..6962
                     /gene="htpG"
                     /locus_tag="ACHKAU_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO5531218.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7143..7787
                     /gene="adk"
                     /locus_tag="ACHKAU_002156"
     CDS             7143..7787
                     /gene="adk"
                     /locus_tag="ACHKAU_002156"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO5531219.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            8023..8985
                     /gene="hemH"
                     /locus_tag="ACHKAU_002157"
     CDS             8023..8985
                     /gene="hemH"
                     /locus_tag="ACHKAU_002157"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO5531220.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8982..9941)
                     /gene="aes"
                     /locus_tag="ACHKAU_002158"
     CDS             complement(8982..9941)
                     /gene="aes"
                     /locus_tag="ACHKAU_002158"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO5531221.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10093..11397
                     /gene="gsk"
                     /locus_tag="ACHKAU_002159"
     CDS             10093..11397
                     /gene="gsk"
                     /locus_tag="ACHKAU_002159"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO5531222.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11530..13206)
                     /gene="ybaL"
                     /locus_tag="ACHKAU_002160"
     CDS             complement(11530..13206)
                     /gene="ybaL"
                     /locus_tag="ACHKAU_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO5531223.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13444..14664)
                     /gene="fsr"
                     /locus_tag="ACHKAU_002161"
     CDS             complement(13444..14664)
                     /gene="fsr"
                     /locus_tag="ACHKAU_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO5531224.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14882..16534
                     /gene="ushA"
                     /locus_tag="ACHKAU_002162"
     CDS             14882..16534
                     /gene="ushA"
                     /locus_tag="ACHKAU_002162"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5531225.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16571..17050)
                     /gene="ybaK"
                     /locus_tag="ACHKAU_002163"
     CDS             complement(16571..17050)
                     /gene="ybaK"
                     /locus_tag="ACHKAU_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO5531226.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17254..18048)
                     /gene="ybaP"
                     /locus_tag="ACHKAU_002164"
     CDS             complement(17254..18048)
                     /gene="ybaP"
                     /locus_tag="ACHKAU_002164"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO5531227.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18186..18527
                     /gene="ybaQ"
                     /locus_tag="ACHKAU_002165"
     CDS             18186..18527
                     /gene="ybaQ"
                     /locus_tag="ACHKAU_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5531228.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18641..21145)
                     /gene="copA"
                     /locus_tag="ACHKAU_002166"
     CDS             complement(18641..21145)
                     /gene="copA"
                     /locus_tag="ACHKAU_002166"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO5531229.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21407..22339
                     /gene="glsA"
                     /locus_tag="ACHKAU_002167"
     CDS             21407..22339
                     /gene="glsA"
                     /locus_tag="ACHKAU_002167"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO5531230.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            22342..23634
                     /gene="ybaT"
                     /locus_tag="ACHKAU_002168"
     CDS             22342..23634
                     /gene="ybaT"
                     /locus_tag="ACHKAU_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5531231.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            23665..23762
                     /locus_tag="ACHKAU_002169"
                     /pseudo
     CDS             23665..23762
                     /locus_tag="ACHKAU_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23759..24166
                     /gene="cueR"
                     /locus_tag="ACHKAU_002170"
     CDS             23759..24166
                     /gene="cueR"
                     /locus_tag="ACHKAU_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO5531232.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(24167..24625)
                     /gene="ybbJ"
                     /locus_tag="ACHKAU_002171"
     CDS             complement(24167..24625)
                     /gene="ybbJ"
                     /locus_tag="ACHKAU_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5531233.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(24622..25539)
                     /gene="qmcA"
                     /locus_tag="ACHKAU_002172"
     CDS             complement(24622..25539)
                     /gene="qmcA"
                     /locus_tag="ACHKAU_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5531234.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            25685..26362
                     /gene="fetA"
                     /locus_tag="ACHKAU_002173"
     CDS             25685..26362
                     /gene="fetA"
                     /locus_tag="ACHKAU_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO5531235.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            26349..27128
                     /gene="fetB"
                     /locus_tag="ACHKAU_002174"
     CDS             26349..27128
                     /gene="fetB"
                     /locus_tag="ACHKAU_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO5531236.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(27191..28045)
                     /gene="cnoX"
                     /locus_tag="ACHKAU_002175"
     CDS             complement(27191..28045)
                     /gene="cnoX"
                     /locus_tag="ACHKAU_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO5531237.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(28106..28915)
                     /gene="ybbO"
                     /locus_tag="ACHKAU_002176"
     CDS             complement(28106..28915)
                     /gene="ybbO"
                     /locus_tag="ACHKAU_002176"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5531238.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(28905..29528)
                     /gene="tesA"
                     /locus_tag="ACHKAU_002177"
     CDS             complement(28905..29528)
                     /gene="tesA"
                     /locus_tag="ACHKAU_002177"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO5531239.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            29499..30185
                     /gene="ybbA"
                     /locus_tag="ACHKAU_002178"
     CDS             29499..30185
                     /gene="ybbA"
                     /locus_tag="ACHKAU_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO5531240.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            30182..32596
                     /gene="ybbP"
                     /locus_tag="ACHKAU_002179"
     CDS             30182..32596
                     /gene="ybbP"
                     /locus_tag="ACHKAU_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO5531241.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         7731 a         8619 c         8782 g         7471 t
ORIGIN      
        1 actgttaagc gtagcgcatc aggcagtttt gcgtttgccc acaatcttag gggacattta
       61 gcggccccat ttatttctca cttttccgcc tcatcatcgc gcgttaattt cttttcataa
      121 tcgcgtttta caatatccag cgcgcgcagc acggtgcggg ctgggatctg attttcttcc
      181 agcagcacaa tcaaatcgac agccagtttg acatcgtcag gggcattttc cagtgacata
      241 ctctctccat tgctagcggg ttaagcgcgc tagcctgttt tcgatttttt ccagcgcatg
      301 gcggcagcgt gccaggcgcg cttcataggc ttccacttca cgatgcagcg tttgctgttc
      361 cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg gcaaccatct cacgtagccg
      421 ccgctcaaaa tcctgatgct gaatacgttt acgctgccag cgggcaattt tcggtggcgc
      481 actgtcccac tcgcgcaatg accaggcgga ggcttcacgc gcgatggctt ccagttgtgc
      541 cgccagatgt tccgccagcc aggccacttg cggcagctgt tgctgctcaa ctgcatgacg
      601 aagcgcagcc agattatcgc ccgcctcatc gagacaagcc tgcagtgttg tcgcacgggt
      661 ttgaaaaagg tgtctgtcaa aacgggcact tagcgtggca aattgcgcta ccggggcaca
      721 acgctgacgc agcgtagcga gctgtccttc cagtttttcc agcagcaggg cggttttcac
      781 gataagacct caatgaaaat gataattgtt atgctaaagt agccactctg taagctgaca
      841 acactcaata tgcaacgaat cattttaatc atcattggct ggctggcggt agtgctgggt
      901 acgctgggcg tggtattacc ggtattaccg acgatgccgt ttatcctgct ggcggcctgg
      961 tgctttgccc gttcttcccc gcgctttcac gcctggttgc tgtaccgctc atggtttggc
     1021 agctatctac gtttctggca gaaacatcat gcgatgccgc gcggcgtcaa accgcgggcg
     1081 attttgctta ttttgctcac gtttgccatt tctctgtggt tcgtccagat gccatgggtg
     1141 cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct atatgtggcg aattccggtg
     1201 attgatgaaa agcaagaaaa gcactgaagc acaacaatcg cagttgcaat tattgcgtac
     1261 agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg gtcaaaggtt aaacaactgt
     1321 tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt aacaagcagg catacactta
     1381 tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag catcaaaagc attcaggact
     1441 acccaaaacc cggcattctt ttccgcgatg tcaccagctt actggaagac ccgaaagctt
     1501 acgctctcag catcgacttg ctggttgagc gttacaaaaa tgcgggcatt accaaagttg
     1561 tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt agctctgggt ctgggcgttg
     1621 gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga aaccatcagt gaaacttacg
     1681 acctggaata cggcaccgat cagctggaga tccacgttga tgccatcaaa ccgggcgaca
     1741 aagttctggt ggtggacgat ctgctggcaa ccggcggcac tatcgaagcg accgttaaac
     1801 tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt cattatcaac ctgttcgatc
     1861 tcggcggcga acagcgtctc gaaaaacagg gcattaccag ctacagcctt gtcccgttcc
     1921 cgggccatta attatcgcca gtcttgtgct gcccacgcta cggacagcac aagatgtgca
     1981 ttcagcctcg ccgttctgac ggggctgtgt tagcattacc ccttcgtgaa tccaccttcc
     2041 agcgtttcag agcctgccaa tgagttatca ggtcttagcc cgaaaatggc gcccacaaac
     2101 ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca ctggcgaacg gcttgtcgtt
     2161 agggcgtatt catcatgctt atcttttttc cggtacccgt ggcgtcggaa aaacctctat
     2221 cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc attaccgcga cgccgtgcgg
     2281 cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt gtcgatctga ttgaaatcga
     2341 cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg ctggataacg tccagtacgc
     2401 tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa gtgcatatgc tgtcgcgcca
     2461 cagctttaac gcactgttaa aaacccttga agagccgccg gagcacgtta agtttctgct
     2521 ggcgacgacc gatccacaga aattgccggt gacgattttg tcgcgctgtt tgcaatttca
     2581 tctcaaggcg ctggatgtcg agcaaattcg ccatcaactt gagcacatcc tcaacgaaga
     2641 acatatcgct cacgagccgc gggcgttgca attgctggcg cgcgccgctg aaggcagcct
     2701 gcgagatgcc ttaagtctga ccgaccaggc gattgccagc ggtgacggcc aggtttcaac
     2761 ccaggcagtc agtgcgatgc tgggtacgct tgacgacgat caggcgctgt cgctggttga
     2821 agcgatggtc gaggccaacg gcgagcgcgt aatggcgctg attaatgaag ccgctgcccg
     2881 tggtatcgag tgggaagcgt tgctggtgga aatgctcggc ctgttgcatc gtattgcgat
     2941 ggtacaactt tcgcctgctg cacttggcaa cgacatggcc gccattgagc tgcggatgcg
     3001 tgaactggcg cgcaccatac cgccgacgga tattcagctt tactatcaga cgctgttgat
     3061 tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg ggcgttgaga tgacgctgct
     3121 gcgcgcgctg gcgttccatc cacgtatgcc tctgcctgag ccagaagtgc cacgccagtc
     3181 ctttgcgccc gtcgcgccaa cggcagtaat gacgccaacc caggtgccgc cgcaaccgca
     3241 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
     3301 ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaaccggc
     3361 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
     3421 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgccag ccaaaaaaga
     3481 agcgtatcgc tggaaggcta ccactccagt gatgcagcaa aaagaagtgg tcgccacgcc
     3541 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctggc
     3601 ggcagaagcc attgagcgcg acgcgtgggc ggcacaggtg agtcaacttt cgctaccaaa
     3661 actggtcgaa caggtggcct taaatgcctg gaaagaggag agcgacaacg cagtatgtct
     3721 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
     3781 agcgttgagc acgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
     3841 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
     3901 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggttct tcgatgcgga
     3961 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
     4021 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
     4081 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
     4141 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
     4201 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
     4261 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
     4321 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
     4381 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
     4441 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgtct
     4501 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
     4561 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
     4621 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttcagg
     4681 ccgttatttt gtgttgatgg ggcacctgtc accgctggac ggcatcggtc cggatgatat
     4741 cggccttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
     4801 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
     4861 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
     4921 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
     4981 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
     5041 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
     5101 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
     5161 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
     5221 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
     5281 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctccgataac ggcgtgggga
     5341 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
     5401 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
     5461 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
     5521 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
     5581 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
     5641 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
     5701 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
     5761 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
     5821 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
     5881 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacatcccg tcccaggctc
     5941 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
     6001 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
     6061 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
     6121 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
     6181 aagacgacgc ggacaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
     6241 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
     6301 cttataccga ttcttctgcg cagaccgtgt ctctggaaga ctacgtttcc cgcatgaaag
     6361 aagggcagga gaaaatctac tacatcactg cagacagcta tgcggcagcg aagagcagcc
     6421 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
     6481 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
     6541 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
     6601 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
     6661 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
     6721 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
     6781 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
     6841 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
     6901 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
     6961 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
     7021 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
     7081 tgttggtggt atcgtttatc gctttttcaa aaaattcgac acattttaag gggattttcg
     7141 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
     7201 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
     7261 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaattggtca
     7321 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
     7381 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
     7441 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
     7501 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
     7561 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
     7621 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
     7681 gctactactc caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
     7741 agccggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaattc gaaagcgcgc
     7801 acggacagtc ccctcgcccc ctcggggaga gggttagggt gaggggaaca ggcccgcaca
     7861 agcaaactta tcagcaatct caggccggat attcattcgg ccttttacaa aaacctcaat
     7921 ctgcgctatt cctttttctg atttgacctc tcacaacaat tagttcttct tcctcacttt
     7981 tccgctacaa ttatcaacaa gttgaatcga taagaggcgg taatgcgtca gactaaaacc
     8041 ggtatcctgc tggcaaacct gggtacgccc gatgccccca cacctgaagc ggtaaaacgc
     8101 tatctgaaac aatttttaag cgacagacgc gtggttgata cctcacggtt gttatggtgg
     8161 ccgttgctgc gcggcgtgat tttgccgctg cgctcgccgc gtgtggcgaa gatgtacgcc
     8221 tcggtctgga tggaagacgg ctcgccgctg atggtttaca gccgccagca acagcaggcg
     8281 ttggcacaac gtttaccgga tacgcccgta gcgctgggca tgagctacgg ctcgccatca
     8341 ctggaaagcg ccgtcgatga actcctggca gagcatgtag atcatattgt ggtgctgccg
     8401 ctttatccgc aattctcctg ttctacggtc ggtgcggtat gggatgaact ggcacgcatt
     8461 ctggcgcgca aacgtagcat tccggggata tcgtttattc gtgattacgc cgataaccac
     8521 gattacatta atgcactggc gaacagcgta cgcgcttctt ttgccaaaca tggcgaaccg
     8581 gatctgctgc tgctctctta tcatggcatt ccccagcgtt atgcagatga aggcgatgat
     8641 tacccgcaac gttgccgcac aacgactcgc gaactggctt ccgcactggg gatggcaccg
     8701 gaaaaagtga tgatgacctt tcagtcgcgc tttggccggg aaccctggct gatgccttat
     8761 accgacgaaa cgctgaaaat gctcggagaa aaaggcgtag gtcatataca ggtgatgtgc
     8821 ccgggctttg ctgcggattg tctggagacg ctggaagaga ttgccgagca aaaccgtgag
     8881 gtcttcctcg gtgccggcgg gaaaaaatat gaatatattc cagcgcttaa tgccacgccg
     8941 gaacatatcg aaatgatggc taatcttgtt gccgcgtatc gctaaagctg agcggtaaag
     9001 aactgagcgc cgtcgcgaag agcctcgtcg gcggttttca tcattcgtga ataatgcaaa
     9061 aaggcatgca gcgtacctgg gtagagtttg aactcacagg gctgctgatg cgccgctaac
     9121 gtctggtaaa gcagacggct gtcatccagc agcggatcga actccgcccc ggcaataaaa
     9181 cagggcggaa cttcgcgagt gagatcatta ttaaacagac agtaatacgg tgactcgcgg
     9241 tccgcgtcgt tgcttaaata cgcctcttca tacatctgca aatcctgttg cgttaagcca
     9301 tcccagacac cgcccaacag acgacgagtc acggaatccc gtaatccgta aagcccatac
     9361 cacagcaaaa cacccgcaat tttaccgcaa tcgatctgtt tatcacgcaa ccacaacgca
     9421 ctggcgagcg ccagcatggc acctgcggaa tcaccggcaa agccaatgcg ggacatattg
     9481 atttgataat cctccgcctg ctggtggaaa taacaacaag cagccacaat ttcctctatc
     9541 gcttgcggaa aacgcgcttc aggtgaaagg gggtaatcaa taccaatcac cgtacattgg
     9601 ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat cgagattgcc gagaataaaa
     9661 ccgcctccat gcaaataaaa tagcgtcgcc gggctatctg gctgcggaca aaagagacgt
     9721 gtttccacct gcccatattt tgttggaacc atgtaagctc tggttgccat ttctggagcg
     9781 cccgcattcc agaatcggcg ctcaagcgtg taatactgtc gttgctcagc aatcgttccc
     9841 gttgcgggcc aggacggtaa atccggctga agagtattca caacggtctt catttcagca
     9901 gaaataaggt ccagaacagg tagtttgttt tccggcttca tacaaaaact cctttcaaat
     9961 tacgtcattg taaggaacca ctgccatgaa aatgcgatcc cgcctgctga tattgaaact
    10021 ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt cgccgctcag ttaaccaccc
    10081 gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca aacattactt ccctgtaaat
    10141 gcacgcgatc cgctgcttca gcaattccag ccagaaaacg aaaccagcgc cgcctgggtc
    10201 gtaggtattg accagacgct ggtcgatatt gaagcgaaag tggatgatga attcattgag
    10261 cgttatggat taagcgccgg gcattcactg gtgattgagg atgacgtagc cgaagcgctt
    10321 tatcaggagc taaaacagaa aaacctgatt acccatcagt ttgcgggtgg caccattggt
    10381 aacaccatgc acaactactc ggtgctcgcg gacgaccgtt cggtgctgct gggcgtcatg
    10441 tgcagcaata ttgaaattgg cagttatgcc tatcgttacc tgtgtaacac ttccagccgt
    10501 accgatctta actatctaca aggcgtggat ggcccgattg gtcgttgctt tacgctgatt
    10561 ggcgagtccg gggaacgtac ctttgctatc agtccaggcc acatgaacca gctgcgggct
    10621 gaaagcattc cggaagatgt gattgccgga gcctcggcac tggttctcac ctcatatctg
    10681 gtgcgttgca agccgggtga acccatgccg gaagcaacca tgaaagccat tgagtacgcg
    10741 aagaaatata acgtaccggt ggtgctgacg ctgggcacca agtttgtcat tgccgagaat
    10801 ccgcagtggt ggcagcaatt cctcaaagac cacgtttcta tccttgcgat gaacgaagat
    10861 gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg catctgacaa ggcgctggac
    10921 tgggtagatc tggtgctgtg caccgccggg ccaatcggct tgtatatggc gggctttacc
    10981 gaagacgaag cgaaacgtaa aacccagcat ccgctgctgc cgggcgctat agcggaattc
    11041 aaccagtatg agtttagccg cgccatgcgc cacaaggatt gccagaatcc gctgcgtgta
    11101 tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa tcatgaacac taatggagcg
    11161 ggggatggcg cattggcagc gttgctgcat gacattaccg ccaacagcta ccatcgtagc
    11221 aacgtaccaa actccagcaa acataaattc acctggttaa cttattcatc gttagcgcag
    11281 gtgtgtaaat atgctaaccg tgtgagctat caggtactga accagcattc acctcgttta
    11341 acgcgcggct tgccggagcg tgaagacagc ctggaagagt cttactggga tcgttaagtt
    11401 atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat ctggcatttg gctctcgatg
    11461 cctgatgcga cgctggcgcg tcttatcatg cctacatatt tttcatattt tacatccggc
    11521 aaccaccgtt taccccgtca ccacctcacc cgccggtggc gtttccagca gttccagcat
    11581 ggtacgggcg atttcacgct cgcccatcac tacctgattc gcaccacgtt cggtgatata
    11641 cgccacttca tcgtcataat gggcgcgggc aataatctca atatccggat ttttcgcgcg
    11701 ggcagatgcc acaatctcac ccgcttcata accgttggga atcgtcagga tcagccattt
    11761 tgcacattcc agatgcgcca gttgcataat ttcttcgttc gccgcattgc ccaatactgc
    11821 gcggaccccg cgctctcgca gctcatcaac acgggttcgt gacgtctcaa tcaccaccag
    11881 cggaatatca gaggcgagca atttctcccc cagcaggctg cctacacgac cgtaacccac
    11941 cagtagcgca tggttgcaaa tatccactgg gatctgcttc tcttcttcga ttgcctcttc
    12001 cagcgtctgc tcttccagcg tttcggtctt cgccagatat ttctccagta gtgcgaacag
    12061 taccgggttg agcataatcg acaggatcgc ccctgccagt actaggtttt gtccggcctg
    12121 cggcagtaaa ttcaatgcca ttcccagtcc cgccaggata aacgcgaact caccaatctg
    12181 cgccaggctg gcggcgatgg ttaatgccgt acgttgggag tgaccaaaca gtcgcaccag
    12241 gaaaaatgcg gctaacgact taccaaacag aataatcgcc agcgtcgcca gcactgccag
    12301 cggttgctga atcagaatta acggatcaaa caacatcccg acggagacaa aaaacagcac
    12361 cgcaaacgcg tcgcgcaatg gcagcgtatc gtgggcggca cggtgactca gttcagactc
    12421 gttcagtacc atcccggcaa agaacgcacc gagtgcaaag gagacatcaa acagctctac
    12481 cgcaccaaag gcaatcccta acgccagcgc cagcaccgac agggtaaaca gctcgcgaga
    12541 accggttgcc gcgctgcgtg ccataatcca cggcaccaga cggcgaccta ccagcatcat
    12601 aatggcgata aatgcgatca ctttgccgat ggtgatcccc atatcgactg caagagtggc
    12661 aaagcccaca tcgccctgtt ccatcattcc tgccactgcg ggcagcaaca ccagcgtcag
    12721 aaccattacc aggtcttcca caatcaacca accgatggcg atttgcccac gctgactgtc
    12781 aattaattgc cgttcttcaa gtgcgcgcag taacaccacg gtactggcgg tggaaagaca
    12841 taaaccgaac acgataccgg tcattaacga ccagcccagc acggcggaga gcgccatacc
    12901 cagcagcgtc gccacggcta tctgggcgat cgcgccggga atggcgatgg actttaccgc
    12961 catcaaatcc ttgagcgaaa aatgcaaacc gacgccgaac atcagcaaaa taacgcccag
    13021 ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag cccggagtga atggtcctgc
    13081 cagcacaccc gctaacagat atcccaccag aggagaaata cgtagtttat tggccagcat
    13141 gccgaggata aaggcgagca caaggccgcc aacaatggtg gtgataagcg gggtggcgtg
    13201 atgcattccg tctccttttc ctggtggtta ttgtccattt ttggccggga aaaccaaaat
    13261 tacaggtaat agtttatgac aatttcattg atgatgttca tgaataatta ttgaattttg
    13321 cagaaaaatg gaattagctg caaaaaaagc acggatcgga aaacagaagg cggtttaaca
    13381 gaggaaaggc ttatggcgac tgtaaaacga tgcagccaaa gtttgccttt ggctgcaatg
    13441 aaatcagtct ttatgccggt tatcaggcag gaatatggtc aacatcccca atagtggcag
    13501 gaaagcacag attttataga ctaactcgat actggtgtga tcggcgataa gccccagaac
    13561 tgccgctccc agacctccca taccaaaagc aaaaccgaaa aagagtccag aaaccatacc
    13621 gatacgtccc ggaagcagct cctgagcgta aaccagaatg gcagagaatg ccgaagcgag
    13681 gataaatcca ataatcaccg ttaaaacccc cgtccagtgc aggctggcgt agggtaaaat
    13741 cagcgtaaac ggcgcaacgc cgaggataga gccccaaatc acatatttcc gcccaatttt
    13801 atcccctaca ggcccgccga tcaccgtacc tgccgcaacg gcaaacagga aggcaaacag
    13861 atgaagctga gcattctgga tagataatcc gaatttttgc atcagataaa aggtgtaata
    13921 gctgctgatg ctcgccatat agaaatattt cgagaaaatg aggattaaca gaatgctgac
    13981 cgccagtacc actttgttgc gcggcagtgg attgataatc gtcgctttgg gttttccttt
    14041 attcattcgg tgctgtgccg agtaccaacg gctgatttgc gccaacacca cgatcgccag
    14101 cagtgccgca agcacaaacc aggcaacgtt gcctttgcca taaggcgcga taatcaccgc
    14161 cgccagcaag ggtcccaggg aactgccaaa gttgccgccg acctgaaaga tagattgcgc
    14221 caggccatgc cgcccgccgg aagccatacg ggctacgcga gaagattccg gatgaaagac
    14281 cgatgaaccg gtaccgacca gcgccgccgc cagcagaact gcgccaaaac tgcccgccag
    14341 cgcaagcagc accagaccac ttaaggtaaa gcacatgcca attggcaacg accacggcat
    14401 cggatattta tcggtccagt agccgaccac tggttgcagt agcgaagagg cgagctggaa
    14461 ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa aattctgact gaagcagcgg
    14521 ataaatcgcc agaatcagcg attggatcat gtcgttcagc agatgtgaga ggctgatagc
    14581 acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac gcagccgcgc ccgccacagg
    14641 ctggggttgt tcactcattg ccataggaaa gtcacttttt cagggttgcg atgtaaagaa
    14701 tgatcttatt tgtgattatt acccgactaa catacctgta tgcgtcgcct gaaggaagtc
    14761 tcaacgccga atacagaatt tctaatctgg atgcagattt atcttcaccg gacgcagact
    14821 tgtctatgat gtcgcgtcat actatttttc aacacgttga aatcaggtca gggagagaag
    14881 tatgaaatta ttgcagcggg gcgtggcgtt agcgctgtta accacattta cactggcgag
    14941 tgaaactgct ctggcgtatg agcaggataa aacctacaaa attacagttc tgcataccaa
    15001 tgatcatcat gggcattttt ggcgcaatga atatggcgaa tatggtctgg cggcgcaaaa
    15061 aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa ggcggtagcg tgctgctact
    15121 ttccggtggc gacattaaca ctggcgtgcc cgagtctgac ttacaggatg ccgaacctga
    15181 ttttcgcggt atgaatctgg tgggctatga cgcgatggcg atcggtaatc atgaatttga
    15241 taatccgctc accgtattac gccagcagga aaagtgggcc aagttcccgt tgctttccgc
    15301 gaatatctac cagaaaagta ctggcgagcg cctgtttaaa ccgtgggcgc tgtttaagcg
    15361 tcaggatctg aaaattgccg tcattgggct gacaaccgat gacacagcaa aaattggtaa
    15421 cccggaatac ttcactgatg tcgaatttcg taagcccgcc gatgaagcga agctggtgat
    15481 tcaggagctg caacagacag aaaagccaga cattattatc gcggcgaccc atatggggca
    15541 ttacgataat ggtgagcacg gctctaacgc accgggcgat gtggagatgg cacgcgcgct
    15601 gcctgccgga tcgctggcga tgatcgtcgg tggtcactcg caagatccgg tctgcatggc
    15661 ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg ccatgcaagc cagatcaaca
    15721 aaacggcatc tggattgtgc aggcgcatga gtggggcaaa tacgtgggac gggctgattt
    15781 tgagtttcgt aatggcaaaa tgaaaatggt taactaccag ctgattccgg tgaacctgaa
    15841 gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg ctttacactc ctgaaatcgc
    15901 tgaaaaccag caaatgatct cgctgttatc accgttccag aacaaaggca aagcgcagct
    15961 ggaagtgaaa ataggcgaaa ccaatggtcg tctggaaggc gatcgtgaca aagtgcgttt
    16021 tgtacagacc aatatggggc ggttgattct ggcagcccaa atggatcgca ctggtgccga
    16081 ctttgcggtg atgagcggag gcggaattcg tgattctatc gaagcaggcg atatcagcta
    16141 taaaaacgtg ctgaaagtgc agccattcgg caatgtggtg gtgtatgccg acatgaccgg
    16201 taaagaggtg attgattacc tgaccgccgt cgcgcagatg aagccagatt caggtgccta
    16261 cccgcaattt gccaacgtta gctttgtggc gaaagacggc agactgaacg accttaaaat
    16321 caaaggcgaa ccggtcgatc cggcgaaaac ttaccgtatg gcgacattaa acttcaatgc
    16381 caccggcggt gatggatatc cgcgccttga taacaaaccg ggctatgtga ataccggctt
    16441 tattgatgcc gaagtgctga aagcgtatat ccagaaaagc tcgccgctgg atgtgagtgt
    16501 ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa agtgccggat gtttgcatcc
    16561 ggcacaatgc ttaatcgcgg cgggcgatat cagcaaattt ggcatcgagg atctttgcca
    16621 gatcgcctgc cgccagttcg atatccagtc cgcgcttgcc gccggaaaca taaatagtgg
    16681 caaattcttg tgcgggggcg tcgataatcg ttggcagacg ttttttctgc cccagtgggc
    16741 taatcccccc aaccaggtat cccgtcgaac gctgcgcgac catcggatcg gccatctcaa
    16801 ctttcttggc acccagcgct tttgctactt ttttaagatc cagttgaccg gcgaccggcg
    16861 taacggccac ggcaaggtgt ttcatatcac cgttcactgc caccagcagc gttttgtaga
    16921 cctgatccgg attcaaacct aattttttga cgacttcatc gccaaaattg gtttcagccg
    16981 gatcgtgctc gtaggtatgg atttgaaacg aaatcttgtt tttttcgagt aatttaactg
    17041 cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg aagcaagcat acgcctgatg
    17101 gcggtccaaa aaaaagagtc atcttgccta agagtattgg caggatggtg agattgagcg
    17161 acaatcgagt tacaccgtcg cttaaagtga cggcataata ataaaaaaat gaaattcctc
    17221 tttgacgggc caatagcgat attggccatt tttttagcgc aacatttgcg gtaaattccc
    17281 ttctccatac aggtgtagtg caccgaccgc gaccacatat cgccccggcg gcatggcgcg
    17341 taatttatcc cgccaggcga gatttcgctg atgcatcagc acatcgtaca gcgactgact
    17401 gaacgtattg ggcagcgtta tttcattatt ttgcggcggt gcattcagcc accagctcat
    17461 catttgttgc agcaaccgtg cattggtgtg ccagtgggtc agtgtatcgt ccagcagcgc
    17521 cagtcctttg tcagggagct ggagcaacat ggcaatctgg ttttcagccc cttccagttc
    17581 aatcacgggt ttatgttgtt gcttcgccgc ctgcaatagc tggtaatcga taccgtattc
    17641 tgcccgcagc cccagttttt gtgcctgcgt cgcctgaaga accatcgcga tttgccacag
    17701 cggttgggta gaaaagagtg agggagaaat gcccatctcc tggctaatgt gctgcagatt
    17761 ttggagttgc tcctcgctaa tgcgctcttc cagcgcctcg caggcaggca aattggcaaa
    17821 aggcgtatcg ctggtggaaa catccgcctc gacgatcagc gcatcggcgt ttttgagctt
    17881 tttgagcaaa cgggtgggca ggggagccat atcgtggcta cccatatgaa tactgccaat
    17941 cagatgaaaa tggcgattgc cgggaagggt gatatcgatg gcaggccagg tgtaatgatt
    18001 accgcgcagc gcggcccaaa gcgtttttac ccggtacaac agatccatac gacctccctt
    18061 tgtgaaatat catgctagcg cgcggggagg gatggtgcaa atggagttgg ggacttgttt
    18121 aatgtgtttg tatgattcag tatattcttg tatcgctatt cacagggaag caacagttaa
    18181 aaactatgaa acaggcaaca agaaaaccga cgacacccgg agatattctt ctctatgaat
    18241 atctggtacc gctcgatttg aaaatcaatg agttagcaga gttgctgcat gttcatcgta
    18301 atagcgtcag tgcactgatc aataacaatc gtaaactcac cactgagatg gcattccgtc
    18361 tggcgaaagt ttttgatacc acagtcgatt tttggctaaa cctccaggcg gcagttgatc
    18421 tttgggaagt tgaaaacaac atgcgcaccc aggaagaatt gggacggatt gaaacagtgg
    18481 ctgaatactt ggcacgccgt gaagagcgtg caaaaaaggt cgcgtaaggc caaaacatcg
    18541 cttcatctcg taggccggat aaggcgttta cgccgcatcc ggcagtcatg cgtcgatgcc
    18601 aaatgtgcca ccctaaagca gcgcatccgc aatgacatac ttattccttc ggtttaaacc
    18661 gcagcaaccg gttggcgtta ctcactacgg taatcgacga gagcgccatt gccgctccgg
    18721 caactaccgg gttaagcagt gttccagtaa acggccacaa aataccggcg gcgaccggaa
    18781 taccgatact gttgtagata aaggcaccga gcaggttctg cttcatgttg cgcagcgttg
    18841 cgcgggaaat cgcgagcgca tccgcaacgc ccatcaggct atggcgcatc agggtaatcg
    18901 ccgcggtttc aatggcaaca tcactgccgc cccccatcgc aataccgaca tccgcctggg
    18961 ccagcgctgg cgcatcgtta atgccgtcgc ccaccattgc cacctgacgt ccttcacttt
    19021 gcaggcgttt aatcgcttcg gctttaccgt ccggcagcac cccggcgatc acttcatcaa
    19081 tcccggcttc tttggcgatc gcattggcgg tggttgggtt atccccggtc aacatcacca
    19141 gacgatatcc cgctttatgc aggcgttgca gcgccgccac gctatcacta cgcaatggat
    19201 cgcgtactgc cagcagggct accgctttcc cgtcaaccgc cagcagcaca ggcgttgccc
    19261 cttgcgatgc ctgagcagta atctccgctt cgatagcttt ggtaccaacc tgttgctcat
    19321 ttaacagcgc ctgattgccc agcaataacg catgaccttc agcttcaccg ctcacgccca
    19381 gcccgcgcaa tgtgcggaaa ccgttgacct gcggcagctg catatcacct gctttatcga
    19441 ggatcgcccg tgccagcgga tggctggaac cttgctccag tgccgccgcc agacgcaatg
    19501 cctgcgcttc atcaacatca gcaaacgttt tcactgcgac aacctgcggc ttcccttcgg
    19561 tcagcgtccc ggttttatcg aacacgacgg tatcgagtgt actggcgcgt tgcagcgcgt
    19621 cggcgtcccg caccagcacg ccaaactcag ccgcccgccc gacgccggaa ataatcgaca
    19681 tcggcgtcgc cagccccagc gcacacggac aggcgataat cagtaccgtg gtggcaatca
    19741 ccagggtata aacaatttgc ggtgccggac caaagaagta ccagattgcc gcactgacca
    19801 gcgcaatcac caccaccaca ggtacaaata cggctgagat tttatccgcc agctgaccga
    19861 tttctggctt gctgctctgg gcctggcgca ccatgcgaat gatccgtgac agcgtggtat
    19921 ggctgccaac cgcactggca cgaaacagca cgctaccgtc ctgtaccact gtcccggcat
    19981 ggacgctttc gccttcgcct ttttgctgcg ggattggttc gcccgtcagc atcgcttcat
    20041 ccagccatgc ttcgccctgg gtaatctcgc catctaccgg cacgcgatcg ccggtcgtca
    20101 ggcgcagcaa catacctggc tgcacttccg ccagaggcac gcttttttca ccttcgtcag
    20161 taaccaggcg cgccgtcggt ggggttaaat cgagtaattt ctccagcgcc ttagaagaac
    20221 gctgacgtgc gcgtgcttcc agcatatggc cgagattgat cagaccgata atcatcgcgc
    20281 tggcttcgta ataaagatgt cgcgcttcca tcgggaacca ctgcggccac aggttgacgc
    20341 tcatcgaata gagccacgcc acgccagtac ccagcgccac cagcgtatcc atcgtcgctg
    20401 caccgttcag caggcttttc catgcactgc ggtaaaaatg gccgccggcg aaaaccatca
    20461 ccgccagggt tatcaggccg ataaccaacc acaggctgcg gttgtcagcg gtgaccatca
    20521 tgttatcgcc aatcatcccc cagaccatca ccgggatacc caccgccagt gcgacaattg
    20581 cctgccagcg gaagcgcttc atcgtagcaa cggcggtttc ctgctggcgc tcacggcgtt
    20641 tcgcgtcatc ttcaatcgct tccgcgccgt agcccgcttt ttccaccgcc tgcactaaat
    20701 cttgtgggga ggcactgccc atcaccagcg cagtacgctc cgccaggttt acccgtgcct
    20761 gagtgacgcc cggtacgctt tgcagcgcat tttgtacgcg ggtgacacag ctggcgcagc
    20821 tcatgccgct cagcagcaac tgctggctgt catcgtcatc ggcggtcgct gccggaagcg
    20881 cctcagaaac cgctgtcagt gcttccgacg ggattgatga ctccgccagc ggtttagcct
    20941 ttgggtggct tacagatgcg tcataacccg cttgtttgat ggtttcgatc agctgttctg
    21001 cactggcagt cccggtaacg tgcgcttcag tgatagacac atccgcttgt tccacatccg
    21061 ggcgctgttc gagactttct ttcacgcgtt taacgcagtg accgcaggac aggccgtcca
    21121 gggtcaggtc gataatttgt gacataaaac actcctttaa gacagttttg actggctgtg
    21181 ataaaggtta aaccttccag caaggggaag gtcaagaaat taataaacca ggcgagtaaa
    21241 agtccgtaaa gattaaaaaa ttggctcaat ttgcatcagg attagacatt tatctctttg
    21301 ttttcctgta gttaagttgc gggtgctaag ttaaatcaag atgcctgaaa atcggcaccg
    21361 gggtgaggaa ttacctcccg catctataaa aaggagttaa caaaagatgt tagatgcaaa
    21421 caaattacag caggcagtgg atcaggctta cacccaattt cactcactta acggcggaca
    21481 aaatgccgac tacattccct ttctggcgaa tgtacctagt caactggcag cagtggctat
    21541 tgtgacctgc gatggcaaca tctatcgcgc gggtgatagt gattaccgct ttgcgctgga
    21601 atccatctcg aaagtctgta cgttagccct tgcgttagaa gatgtcggcc cgcaggcggt
    21661 acaggacaaa attggcgctg acccgaccgg attgcccttt aactcagtta tcgccttaga
    21721 gttgcatggc ggcaaaccac tgtcgccact ggtaaatgct ggcgctattg ccaccaccag
    21781 cctgattaac gctgaaaata ttgaacaacg ctggcagcga attttacata tccaacagca
    21841 actggctggt gagcaggtag cgctctctga tgaagtcaac cagtcggaac aaacaaccaa
    21901 cttccataac cgggccattg cctggctgct gtactccgcc ggatatctct attgtgatgc
    21961 aatggaagcc tgtgacgtgt acacccgtca gtgctccacg ctcatcaata ctgttgaact
    22021 ggcaacgctt ggcgcgacgc tggcggcggg tggtgtgaat ccgctgacgc ataaacgcgt
    22081 tcttcaggcc gacaacgtgc cgtacattct ggccgaaatg atgatggaag ggctgtatgg
    22141 tcgctccggt gactgggcgt atcgcgttgg tttaccgggc aaaagcggtg taggtggcgg
    22201 tattctggcg gtcgtccctg gcgtgatggg aattgccgcg ttctcaccac cgctggacga
    22261 agaaggcaac agtgttcgcg gtcaaaaaat ggtggcatcg gtcgctaagc aactcggcta
    22321 taacgtgttt aagggctgat catgatgaac acggaaggta ataacggtaa caaacctctc
    22381 ggtctatgga acgtcgtttc catcggcatt ggggcaatgg tgggggcggg gatcttcgcg
    22441 ctgctggggc aggctgcatt gctaatggaa gcctcgacct gggtcgcctt tgcttttggc
    22501 ggtattgtgg cgatgttttc cggttatgcc tatgcgcgtc tgggggcgag ctatcccagc
    22561 aatggcggca ttatcgactt ctttcgtcgc ggattaggca acggcgtctt ttcgctggcg
    22621 ctctcgttac tgtacctgtt gacgctggcg gtgagcatcg ccatggtcgc ccgtgctttt
    22681 ggcgcttatg ccgtgcagtt tttgcatgaa ggcagccagg aggagcacct tattttgctc
    22741 tacgcgttgg ggatcattgc ggtgatgacg cttttcaact ccttaagcaa ccatgcggta
    22801 gggcggctgg aagtgatcct cgtcggcatt aaaatgatga tcctgttatt gctgattatt
    22861 gccggtgtct ggtcgctgca accggcgcat atttccgtct ctgcgccccc cagctccggt
    22921 gcgttcttct cctgtatcgg gataaccttc cttgcctatg cgggctttgg catgatggcg
    22981 aacgcggcgg ataaagtgaa agatccgcag gtcattatgc cgcgggcgtt tctggtggcg
    23041 attggcgtta ccacgttgct ttatatctcg ctggcactgg ttttgcttag cgatgtatcg
    23101 gcattagagt tagaaaaata tgccgatacc gccgtagcgc aggctgcttc tccgctgctc
    23161 ggacatgtgg gttatgtgat cgtcgtcatc ggcgctttac tggcgacggc ttcagccatt
    23221 aacgcgaacc tgttcgccgt gtttaacatc atggacaaca tgggcagcga acgcgaactg
    23281 ccgaagctaa tgaataaatc cctgtggcgg cagagtacct ggggcaacat cattgtcgtg
    23341 gtgttgatta tgctgatgac ggcggcacta aatttaggct cactcgccag cgttgccagc
    23401 gccacctttt tgatttgcta tctggcggtg tttgtggtgg cgatccgcct gcgtcatgat
    23461 attcacgcct cgttgccgat tcttatcgtt ggtacgttgg tgatgttgtt ggtgatcgtt
    23521 ggctttatct acagtctgtg gtcccagggt agccgtgcgt tgatatggat tattggctca
    23581 ctcttactca gccttattgt ggcaatggtc atgaagcgca ataaaaccgt ataacatctc
    23641 tctgtgcgca gtacttcctg tattattgtg gtggcggtcg atattcgcac tggcaaaaaa
    23701 acgtgcttga atatctgttg aaacccttta acaaagcaca ggaggcgttg cgcgaacgat
    23761 gaacatcagc gatgtagcaa aaattaccgg cctgaccagc aaagccattc gcttctatga
    23821 agagaagggg ctggtgacgc cgccgatgcg cagcgaaaac ggttatcgca cctacacgca
    23881 gcagcatctc aacgaactga ccttactgcg ccaggcacgg caggtgggct ttaacctgga
    23941 agagagcggc gagctggtga atctgtttaa cgacccgcag cggcacagcg ccgacgtcaa
    24001 acggcgcacg ctggagaagg tggcggagat cgaacgacac attgaggagc tgcaatccat
    24061 gcgcgaccag ctgctggcac tggcgaatgc ctgccctggc gatgacagcg ccgactgccc
    24121 gattatcgaa aatctctccg gctgctgtca tcatcgggca gggtgattaa gacgagacgg
    24181 ctcggatatg tagggttatc ccttctatcg caatgacttc aacatgcgta cctgcaccga
    24241 gatcctcgct ggcgctgaca ggccatgaac tgtcaccgac gcgcatatga ccgcgcccgt
    24301 tgaccagcgg agattccagc acaaaacgtc ggccaatcag ctgctgcccg cgctggttta
    24361 aatgactatc gctgtgcttt tgttcgcgca cccgccgcga caaccatttc caccacagcc
    24421 aggcggcgag cagcgtcagg atggcaaaca ttaccccttg ccactcccaa cccagcggca
    24481 ccagccagac caccaggccg gtaatcaccg ctgccacgcc gctccacaac aaataaccat
    24541 ttccgcccag catctcggct gccagcagca aaccgccgag actgagccag aaaatatgtg
    24601 gatgaacgac cattaactcc atcatggctg agtccgtttg ttggcgctgt ctttcaccag
    24661 ctcggcaatc ccggcaatcg accccatcag gctgctggcc tctaatggca tcatcactac
    24721 tttgctgtta ctggaggaac cgatctgctg taacgcttcg gtgtatttct gcgctacgaa
    24781 gtagttcacc gcctgaatat caccggaggc gatagcttca gacaccattt tggtggcgcg
    24841 ggcttctgct tcggcggaac gttcacgcgc ttcagcctgt aaaaacgccg actgacgttc
    24901 gccttccgct ttcaggattt gcgactgttt ttcaccttcg gctttgagga tttccgcctg
    24961 acggatccct tccgcttcaa gaatgtaagc gcgtttggta cgttccgctt tcatctgcgc
    25021 gttcattgaa gagataagct ctgccggtgg gcgcacgtcg cgaatttcaa tacgggtgac
    25081 tttaatcccc cacgggttgg tggcttcatc gacaatacgc agcaggcgtg agttgatgct
    25141 gtcgcgctga gagagcattt cgtcaagttc cattgaaccc aacacggtac ggatgttagt
    25201 catggtcagg ttgatgatcg ccagctccag attgctgact tcataagccg cgcgcggcgc
    25261 gtcaatcacc tgaataaaac agactgcgtc gatggtaacg ttggcgttat ctttcgagat
    25321 aacttcctgg gaagggatat cgagcacttg ctccatcata ttgatcttgc gaccaatgcg
    25381 atccataaac ggcaccacca gactgagccc cggctgtaac gttttggtat agcgaccaaa
    25441 acgttctacc gtccattgat agccctgcgg cacgattttg acgcccgcgc cgacaatgac
    25501 cagcgcgaca aaaatgagaa tcgggataaa gataagcatc ggaaaaacct cctgttgtac
    25561 cgtccataat cagcaaaatt gctgcttgat taaacaaatt atacctgatt actgaaagag
    25621 agttccccct tattcctgcg aaggataaac tgtttttagt aaaaatcaga aaaagggaac
    25681 agtgatgcag gaaaatagtc ctttacttca gctacaaaac gtaggatatc tggcgggtga
    25741 tgcgaagatt cttaataaca tcaatttttc gctgcgtgct ggcgaattta agttaattac
    25801 cggtccttcc ggttgtggca aaagtacgct gctaaaaata gttgcttcat tgatcagccc
    25861 aaccaacgga acgttactgt ttgaaggtga ggatgtcagc acactaaagc cagaaatcta
    25921 ccgccagcaa gtctcttact gcgcccagac gccgacgctg tttggcgata cggtatacga
    25981 taatctgatc tttccctggc agatccgtaa ccagcagcct gacccggcca tttttctcga
    26041 ttttctcgaa cgcttcgctt tgccggacag cattttgacg aaaaatatcg ccgagctatc
    26101 tggtggtgaa aaacaacgca tctcactgat tcgtaacctg caatttatgc cgaaggtttt
    26161 attgctggat gaaatcacca gtgcgctgga tgaaagtaat aaacataacg ttaatgagat
    26221 gatccatcgt tatgtgcgcg agcaaaatat tgccgtgctg tgggtgacac acgataaaga
    26281 cgaaattaat catgcggata aagtgattac actgcaaccg catgccggag aaatgcagga
    26341 agcacgctat gaactcgcat aatattacta acgaatcatt ggcactggca ttaatgctgg
    26401 tggtggtggc aatcttaatt agccataaag aaaaactggc gctggagaaa gatattttct
    26461 ggagcgtcgg gcgagcgata attcagctga ttattgtcgg ctatgtgctg aagtatattt
    26521 tcagcgtgga tgatgccagc ctgacattat tgatggtgtt atttatctgc tttaatgcgg
    26581 cgtggaacgc gcaaaaacgc agtaaatata ttgctaaagc ttttatctca tcgtttattg
    26641 ccattacggt cggggcggga attaccctgg cggtgctgat tctctctggg tcgattgaat
    26701 ttatcccgat gcaggtgatc cctatcgccg ggatgattgc cggtaacgcc atggtagcgg
    26761 tggggttgtg ttacaacaat ttagggcaac gggtcattag cgaacagcaa cagattcagg
    26821 agaaactgag tcttggtgcg acgccgaagc aggcttcagc gatattgatt cgcgacagta
    26881 ttcgcgcggc tttaattccg acggtcgatt cagcaaaaac ggttggctta gtgagtttac
    26941 ctggaatgat gtccgggctg atatttgccg ggattgatcc ggtgaaggcg attaaatatc
    27001 agattatggt gacctttatg ctgctctcaa ccgccagctt gtcgaccatt attgcctgct
    27061 atttaaccta tcgtaagttt tataattcgc gccaccagtt ggtggtgacg caattgaaga
    27121 agaaatgatg cgatgccgga tgcgcagcat cgcatccggc gttgtggttc atgtgccgat
    27181 caacggaata tcaatacaac aatgcataca gctggcggcg atacttcgac gccagtgcat
    27241 cacccgtacc cagcgcagcg aggatctcct gaaacgtttt acgcgtctga ccgtctgcgg
    27301 cggtgagatc tttacgcaga tgcccgaaca gcaactccag cgcctcttca ttgcgcccaa
    27361 cctggtgcag ttgcagagcc agttgcgtcg ccagtgcggc atcttctgga ttctccgcca
    27421 cctgctgttg caactgttga atttccggcg tatcagccgc ctgcttcagc agttcgattt
    27481 gcgccaccag cccctggtag cgggtgtcct gatcctgcaa cggaatggtt ttcagcaccg
    27541 tttccgcatc ttcagaacgg ttcagcgcaa tcagcgtttc tgccagcagc aggccgatct
    27601 ccccgttctg attcgacaac tgccaggcgt ctttcagcaa tggcagggca tcggtgtaat
    27661 tgccttcctg catcagttgc atcgcctgct gcgctttcag ctcttcttcg cgcggcagca
    27721 ctttatccag cagggcgcgg atcgcctctt ccggttgcgg cccctggaag ccatctaccg
    27781 gttgcccgtt ctggaacaga tacacggtcg gaatcgcgcg cagaccaaac tgggcggcaa
    27841 tcatctgctc cgcgtcgcag tccagcttcg ccagaataaa ttgcccgttg tactgcgccg
    27901 cgaggctttc caggatcggg gttaactgca aacagtgctg gctacgttca gaccaaaaat
    27961 agaacagcac cggagtggtc atcgactgtt caagaacctg ttgcaggtta gattcgttaa
    28021 tgttgacaat attttctacg gacatggagt cgctctctgt tgtcgatatt ttctttgaca
    28081 tgggggctta agcgcgcgct tcaactcacc cctgcaatat tttgtccatc acgcgccccg
    28141 gcagcaggcg cttgagcacc attactgccc aggtcaccag cgttaccgga tagcgcatct
    28201 tcggcttctc gctaataaaa gcatggcgta ctttgtccac caccgcttcc ggtcccaacg
    28261 taaagcgggc ggcgatgccg ggattttcga ctggtttatc actttgcgtc tggttgacgt
    28321 tgtcggtgaa gcgagtacga atgggaccgg gttcgatcag gctgacttta attccgctgt
    28381 ggcgcagctc catgcgcagt gcgtctgacc acgcctccag cgcatattta ctggccgcgt
    28441 aagcgccacg acccggcgtg gagattaatc ccatcaccga tgatgtcatc acaatacgcc
    28501 cttcaccgtg cggtaacatc gcgggtaaca ggcgcatggt gagctggtgt gcgccgaaaa
    28561 agttggcgga aaactgctgt tccatctgcg cacggctgat ggtggaaagg gggccataca
    28621 tgccgaatcc ggcattgtta aagatcccat acagacaatt atcggtcagg gcgatcacct
    28681 cgtctgctgc gcgatcaaca ctttctggtg aatccagatc gatcaacacg ccggtaaatc
    28741 ccatgttgtt catacgctca acatcatccg gttttcggca acctgccagc acatgaaaac
    28801 cctggcgttt taattcgagc gcgctttcca ggccaattcc actggaacat ccggtaatta
    28861 agaccgattt ttgcataact ttacctgtca ggatctccgt tgctttatga gtcatgattt
    28921 actaaaggct gcaactgctt cgccatccag tcggcaataa acggctgggc gtcgcggttg
    28981 ggatgaatac cgtcatcctg catccattgt ggcttgaggt agacctcttc cataaaaaag
    29041 ggcagcagcg gaacatcaaa ctctttggcg agtttggggt aaatggcgct aaaggcttca
    29101 ttataacggc gaccatagtt tgcaggcaga cgtatttgca ttaacaatgg ttcagcgttg
    29161 gcggctttga catcctgcaa aatctggcgc agcgtttgct cggtttgctg tggctgaaaa
    29221 ccacgcaaac cgtcattgcc gcccagttca accagcaccc agcgcggctg atgctgtttc
    29281 agcagagccg gaaggcgcgc cagtccttgt tgcgaggtgt cgccgctgat gctggcatta
    29341 actaccgacg ttttactctg ccacttatca ttcaacaagg caggccaggc cgcgctggca
    29401 gacattcgat acccggcgct caggctatca cccagaatca ataacgtgtc cgctgcggcg
    29461 gcacggaagg ttaacaggac caggaacagg aagggcaaat gccagcggaa aacattgttg
    29521 aagttcatca tcttaagaag tccgtcggtc agggggagca tgaactctcc atcctcaccg
    29581 gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact ggtgggcgaa tcgggatcgg
    29641 gtaagtcaac cttgctggcg atcctcgccg ggcttgatga cggcagcagt ggcgaagtga
    29701 gtctggtggg acaaccgcta cataatatgg acgaagaagc gcgggcaaag ttgcgcgcga
    29761 agcacgtcgg ctttgttttt cagtcattta tgttaattcc tacccttaac gcgctggaaa
    29821 atgtcgagct tccggctctg ctgcgcggtg agagtagcgc ggaaagtcgt aacggggcga
    29881 aagcgttgct cgaacagtta gggctgggta aacgtctgga tcatcttccg gcacagcttt
    29941 ccggcggtga acagcaacga gtggcgctgg cacgagcctt taatggtcga cctgatgtgc
    30001 tgtttgccga cgaacccacc ggcaaccttg accgccagac gggcgataaa attgccgacc
    30061 tgctgttttc cctcaaccgt gaacatggca ccacgttgat tatggtgact cacgacctgc
    30121 aactggcggc acgctgcgac cgctgcttac ggctggtgaa cgggcagttg caggaggaag
    30181 catgattgca cgttggttct ggcgcgaatg gcgctcgccg tcgctattaa ttgtctggct
    30241 ggcgctaagc ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg atcgcatgga
    30301 gaagggctta agccagcaaa gccgtgagtt tatggcgggc gatcgggcgt tgcgcagttc
    30361 gcgcgaagtg ccgcaagcat ggctggagga agcgcaaaag cgcggcctga aagtcggcaa
    30421 gcagctgact ttcgccacca tgacatttgc aggcgacaca ccgcagctgg cgaacgtcaa
    30481 agcggtggat gatatctacc cgatgtatgg cgatctgcaa actaatcccc ctggcctgaa
    30541 accgcaggcg ggcagcgtat tgctggcccc acgcctgatg gcactgctta acctgaaaac
    30601 gggcgacacc attgacgtgg gcgatgccac cttgcggatt gccggagaag tgattcagga
    30661 accggattcc ggttttaacc ccttccagat ggctccgcgt ctgatgatga atctggcgga
    30721 tgtcgataaa accggagccg tgcagccggg gagtcgggtc acctggcgtt ataaattcgg
    30781 cggcaacgag aaccagctcg acggctatga gaaatggttg ttacctcagc ttaaacccga
    30841 acaacgctgg tacggtctgg aacaggacga aggcgcgctg gggcgatcga tggaacgctc
    30901 gcaacagttc ctgctgcttt cggcgcttct gaccttgctg ctggcagtgg cagcggtggc
    30961 ggtagcgatg aatcattact gtcgcagtcg ctacgatctg gtggcgatcc tcaaaacgct
    31021 gggggcaggg cgagcgcaac tgcgtaagct aatcgtcggt cagtggttga tggtgctggc
    31081 gctttcagcc gttaccggtg gggcgatagg cctgttgttc gaaaacgtgt tgatggtgct
    31141 gctcaagccc gttctgcctg ctgcactacc gccagccagc ctctggccgt ggctgtgggc
    31201 gctgggtacc atgacggtca tctcgctgct ggtggggcta cgaccgtacc gcctgttgct
    31261 ggcaacgcag cctttacgcg tattacgtaa tgatgtggta gcgaacgtct ggccgctgaa
    31321 gttttatctg ccgattgtca gtgtggtggt tgtgctgctg ctcgccggat taatgggtgg
    31381 cagcatgctg ctttgggcgg tgctggcggg cgcggtagta ctggctttgc tgtgcggtgt
    31441 gctgggctgg atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc tacctctgcg
    31501 cctggcggtt agccgcctgt tacgtcagcc gtggtcaaca ttaagccagc tttcggcatt
    31561 ttcgctctcc tttatgctgc tggcgctgtt gctggtattg cgtggcgatc tgcttgaccg
    31621 ctggcagcag cagctacctc cagaaagccc gaactacttt ttaattaaca ttgccacaga
    31681 acaggtagaa ccgctaaaag cgttcctcgc ggaacatcag atagtcccgg aatcatttta
    31741 tccggtagtg cgggcgcggc tgacggcgat taacgataag ccgacagaag gtaatgaaga
    31801 tgaggcgctt aaccgcgaac tcaatcttac ctggcaaaat acgcgacccg atcataatcc
    31861 gattgtcgcc ggtaactggc cgccaaaagc cgatgaagtg tcgatggaag aggggctggc
    31921 gaaacgctta aacgttgccc tcggtgatac cgtgactttt atgggcgata cccaggagtt
    31981 ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc ctaatttcta
    32041 ttttattttc cctgaagggg cattagacgg gcaaccgcag agctggctta ccagtttccg
    32101 ctgggagaat ggcaacggca tgttgacaca actcaaccgc cagttcccga ccattagcct
    32161 gttagatatt ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg taagtcgggc
    32221 gctggaggtg atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc tggcacaggt
    32281 gcaggtggga atgcgccagc gtcatcagga gctggtggta tggcgcacac tcggtgcggg
    32341 gaaaaaactg ctgcgcacga cattgtggtg tgagttcgcc atgcttgggt ttgtttccgg
    32401 cctggtggcc gcaattggtg cggaaacggc actggcagtg ttgcaggcga aagtgtttga
    32461 tttcccgtgg gagccagact ggcgattgtg gattgttctg ccgtgcagcg gtgcgctgct
    32521 gctgtcgctt ttcggcggct ggctgggtgc gcgactggtt aagggtaagg cgctgttcag
    32581 gcagtttgcg gggtgatgag agt
//
LOCUS       DAZFJF010000042        32240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFJF010000042 DAZFJF010000000
VERSION     DAZFJF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.042"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            9..611
                     /gene="yebB"
                     /locus_tag="ACHKAU_002180"
     CDS             9..611
                     /gene="yebB"
                     /locus_tag="ACHKAU_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO5531242.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(613..1134)
                     /gene="ruvC"
                     /locus_tag="ACHKAU_002181"
     CDS             complement(613..1134)
                     /gene="ruvC"
                     /locus_tag="ACHKAU_002181"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO5531243.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1169..1909)
                     /gene="yebC"
                     /locus_tag="ACHKAU_002182"
     CDS             complement(1169..1909)
                     /gene="yebC"
                     /locus_tag="ACHKAU_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5531244.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1938..2390)
                     /gene="nudB"
                     /locus_tag="ACHKAU_002183"
     CDS             complement(1938..2390)
                     /gene="nudB"
                     /locus_tag="ACHKAU_002183"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO5531245.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2508..4280)
                     /gene="aspS"
                     /locus_tag="ACHKAU_002184"
     CDS             complement(2508..4280)
                     /gene="aspS"
                     /locus_tag="ACHKAU_002184"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO5531246.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4590..5156
                     /gene="yecD"
                     /locus_tag="ACHKAU_002185"
     CDS             4590..5156
                     /gene="yecD"
                     /locus_tag="ACHKAU_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5531247.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5153..5971
                     /gene="yecE"
                     /locus_tag="ACHKAU_002186"
     CDS             5153..5971
                     /gene="yecE"
                     /locus_tag="ACHKAU_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO5531248.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6024..6419
                     /gene="yecN"
                     /locus_tag="ACHKAU_002187"
     CDS             6024..6419
                     /gene="yecN"
                     /locus_tag="ACHKAU_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO5531249.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6460..7203
                     /gene="cmoA"
                     /locus_tag="ACHKAU_002188"
     CDS             6460..7203
                     /gene="cmoA"
                     /locus_tag="ACHKAU_002188"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO5531250.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7200..8171
                     /gene="cmoB"
                     /locus_tag="ACHKAU_002189"
     CDS             7200..8171
                     /gene="cmoB"
                     /locus_tag="ACHKAU_002189"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO5531251.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8336..10765)
                     /gene="torZ"
                     /locus_tag="ACHKAU_002190"
     CDS             complement(8336..10765)
                     /gene="torZ"
                     /locus_tag="ACHKAU_002190"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO5531252.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10790..11890)
                     /gene="torY"
                     /locus_tag="ACHKAU_002191"
     CDS             complement(10790..11890)
                     /gene="torY"
                     /locus_tag="ACHKAU_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO5531253.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(12278..13024)
                     /gene="cutC"
                     /locus_tag="ACHKAU_002192"
     CDS             complement(12278..13024)
                     /gene="cutC"
                     /locus_tag="ACHKAU_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO5531254.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(13038..13604)
                     /gene="yecM"
                     /locus_tag="ACHKAU_002193"
     CDS             complement(13038..13604)
                     /gene="yecM"
                     /locus_tag="ACHKAU_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5531255.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13820..15553
                     /gene="argS"
                     /locus_tag="ACHKAU_002194"
     CDS             13820..15553
                     /gene="argS"
                     /locus_tag="ACHKAU_002194"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO5531256.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(15595..15639)
                     /gene="yecV"
                     /locus_tag="ACHKAU_002195"
     CDS             complement(15595..15639)
                     /gene="yecV"
                     /locus_tag="ACHKAU_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO5531257.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            15730..16218
                     /gene="yecT"
                     /locus_tag="ACHKAU_002196"
     CDS             15730..16218
                     /gene="yecT"
                     /locus_tag="ACHKAU_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO5531258.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(16338..16730)
                     /gene="flhe"
                     /locus_tag="ACHKAU_002197"
     CDS             complement(16338..16730)
                     /gene="flhe"
                     /locus_tag="ACHKAU_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO5531259.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(16730..18808)
                     /gene="flhA"
                     /locus_tag="ACHKAU_002198"
     CDS             complement(16730..18808)
                     /gene="flhA"
                     /locus_tag="ACHKAU_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5531260.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(18801..19949)
                     /gene="flhB"
                     /locus_tag="ACHKAU_002199"
     CDS             complement(18801..19949)
                     /gene="flhB"
                     /locus_tag="ACHKAU_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO5531261.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(20151..20795)
                     /gene="cheZ"
                     /locus_tag="ACHKAU_002200"
     CDS             complement(20151..20795)
                     /gene="cheZ"
                     /locus_tag="ACHKAU_002200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO5531262.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(20806..21195)
                     /gene="cheY"
                     /locus_tag="ACHKAU_002201"
     CDS             complement(20806..21195)
                     /gene="cheY"
                     /locus_tag="ACHKAU_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO5531263.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(21210..22259)
                     /gene="cheB"
                     /locus_tag="ACHKAU_002202"
     CDS             complement(21210..22259)
                     /gene="cheB"
                     /locus_tag="ACHKAU_002202"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO5531264.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(22262..23122)
                     /gene="cheR"
                     /locus_tag="ACHKAU_002203"
     CDS             complement(22262..23122)
                     /gene="cheR"
                     /locus_tag="ACHKAU_002203"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO5531265.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(23141..24745)
                     /gene="tap"
                     /locus_tag="ACHKAU_002204"
     CDS             complement(23141..24745)
                     /gene="tap"
                     /locus_tag="ACHKAU_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO5531266.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(24791..26452)
                     /gene="tar"
                     /locus_tag="ACHKAU_002205"
     CDS             complement(24791..26452)
                     /gene="tar"
                     /locus_tag="ACHKAU_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO5531267.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(26597..27100)
                     /gene="cheW"
                     /locus_tag="ACHKAU_002206"
     CDS             complement(26597..27100)
                     /gene="cheW"
                     /locus_tag="ACHKAU_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO5531268.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(27121..29085)
                     /gene="cheA"
                     /locus_tag="ACHKAU_002207"
     CDS             complement(27121..29085)
                     /gene="cheA"
                     /locus_tag="ACHKAU_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO5531269.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(29090..30016)
                     /gene="motB"
                     /locus_tag="ACHKAU_002208"
     CDS             complement(29090..30016)
                     /gene="motB"
                     /locus_tag="ACHKAU_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO5531270.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(30013..30900)
                     /gene="motA"
                     /locus_tag="ACHKAU_002209"
     CDS             complement(30013..30900)
                     /gene="motA"
                     /locus_tag="ACHKAU_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5531271.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(31027..31605)
                     /gene="flhC"
                     /locus_tag="ACHKAU_002210"
     CDS             complement(31027..31605)
                     /gene="flhC"
                     /locus_tag="ACHKAU_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO5531272.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(31608..31958)
                     /gene="flhD"
                     /locus_tag="ACHKAU_002211"
     CDS             complement(31608..31958)
                     /gene="flhD"
                     /locus_tag="ACHKAU_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO5531273.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         7594 a         8767 c         7844 g         8035 t
ORIGIN      
        1 atgacaacgt gaatattaat taccctgctg aatatgaaat tggtgatatc gtctttacat
       61 gtataggtgc tgccttattt ggtcaaatat cagctgcatc aaactgctgg agtaatcacg
      121 tcgggatcat tatcggtcat aacggtgaag actttctggt tgcagaaagc cgtgttcccc
      181 tctcaaccat cactacgcta tcccgtttta ttaaacgctc tgctaatcaa cgctatgcta
      241 taaagcgatt agacgccggg ctgacagaac aacagaaaca acgaattgtt gaacaggttc
      301 cctcccggct acgcaaactt taccacacgg gttttaaata cgaatcttcc cgccagttct
      361 gttcaaaatt tgtctttgat atttataaag aggcgctatg tattccggtg ggtgaaatag
      421 agacgtttgg acaactgtta aatagcaatc cgaatgcaaa actcactttc tggaaattct
      481 ggttcttagg ttctattccg tgggagcgta aaaccgtcac gccagccagt ttgtggcatc
      541 atccgggttt ggtgttgatt cacgcggagg gagttgaaac gcctcagcct gaactgaccg
      601 aggcggtata acttaacgca gtcgccctct cgccaggttc agccgcgatt cgctcatctg
      661 cattgcattc tggctgacgt gacaatgggt aatcgcgata gctaacgcat cagcggcatc
      721 cgcttgtgga ttagcgggca gtttcagcaa ggtgcggacc atatgctgca cctggctttt
      781 ctcggcgcta ccaataccta ccaccgtttg ctttacctga cgtgccgcgt attcaaatac
      841 cggcaattcc tgattcaccg ccgccacaat cgccacgccg cgcgcctggc ccagtttcag
      901 ggctgagtca gcgttcttcg ccataaagac ttgttcaatg gcgaaataat caggctggaa
      961 ctgggtgatg atttccgtca cgcccgcata gatgagcttc agacgagacg gtaaatcatc
     1021 cactttggtg cgtatgcatc cactacccag gtaggacagt tgcctgccta cctggcggat
     1081 gacgccatag ccggtcacgc gcgaacccgg atcaatgccg agaataatag ccatcacgcg
     1141 tctccgtttt gctgtttagc aggcctcatc agagagtcgc tgcaacctca tcagagattt
     1201 caccgttatg gtaaacttcc tgcacgtcgt cgcaatcttc cagcatatcg atcagacgca
     1261 tcagtttcgg tgcggtttct gcatccatat cagctttggt ggacgggatc atggaaactt
     1321 ccgcgctgtc tgctttcaga cctgccgctt ccagagcgtc gcgcacttta cccatttctt
     1381 cccatgcggt gtagacatca atcgcgccgt catcataggt cacaacgtct tcagcacctg
     1441 cttccaaagc tgcttccatg atggtgtctt catcgccttt ctcgaaggag atcacgcctt
     1501 ttttgctgaa cagataggct acggaaccat cagtaccgag gttaccgcca catttgctga
     1561 atgcatgacg cacttcagca acggtacggt tgcggttgtc agacagacat tcaatcatga
     1621 ttgccgtgcc gccaggaccg taaccttcgt agatgatggt ttccatgttt gcatcatcat
     1681 caccgcccac accacgtgca attgcgcggt tcagtgtgtc gcgggtcatg ttgttagaca
     1741 gtgctttatc aattgctgca cgcagacgcg ggttagcgtc cggatcgcca ccgcccagct
     1801 tagccgcggt taccagctca cgaatgattt tagtgaagat tttaccgcgc tttgcatcct
     1861 gcgcagcttt acgatgtctg gtgttggccc atttactatg acctgccata aaaatatctc
     1921 cagatagccc tgcctgttca ggcagcgtta atgacaaact gttcaatcgc ctgccggttg
     1981 ctccaggact tagtgagcgc cgccgcagca ggcgcatcaa gccacttgta agccagatgt
     2041 tcagtgaaaa cgatctgccg ctcgtgcgga agcgcaagac agaaccatga ttccgtatta
     2101 cgcgtcacgc ccggcgcata gcgatgacgt aaatgtgaaa aaatttcaaa ctctaccgtg
     2161 cgctgacagt caattaaggt cagttgttca gcgacaacat caatggtgac ctcttccttt
     2221 acttcgcgca tggcagcttg cggcgcggtt tcaccctctt ccacgctgcc ggttaccgac
     2281 tgccagaaat cgggatcgtc acgccgctgc aacatcagca cccgtttcgt atcttgtgca
     2341 tagatgacca ctaagatcga aacgggacgc ttatacactt tatccttcac gctgcctctt
     2401 cgttgactgc cttcgctcat cccattcaca tagttatcta tgctcatggg agttcactca
     2461 gttgccgcct tgatgcaacg cgaatgattt cgtgtatttg agtcatatca gttattctca
     2521 gccttcttca caacctgaat gctcagctca gccagtgcag tcgggttagc aaagctcggt
     2581 gcttcagtca tcagacacgc tgccgccgtg gttttcggga aggcgataac gtcacggata
     2641 ttgtcggtgc cagtcagcag catggtcaga cggtcaagac cgaatgccag acctgcgtgc
     2701 ggcggagtac cgtatttcag ggcgtcgagc aggaagccga atttctcgcg ctgttcctct
     2761 tcgttgatac ccagaatacc aaacaccgtc tgctgcatat caccattatg gatacgtact
     2821 gaaccaccgc ccacttcgta accattgatg accatatcgt aagcgttcgc caccgcattt
     2881 tccggtgcag ctttcagttc tgcagcagtc atgtctttcg gtgaggtgaa cggatggtgc
     2941 attgccgtca ggccgccttc accgtcgtct tcaaacatcg ggaagtcgat aacccacagc
     3001 ggtgcccatt tgctttcgtc ggtcagacca aggtctttac ccactttcag gcgcagtgcg
     3061 cccatcgcgt cggcaacaat tttcttgttg tcggcaccga agaaaatcat atcgccatct
     3121 tgcgcggcag tacgatccag gatggcttcg atgatttctg cattaaggaa cttcgctacc
     3181 gggctgttga taccttccag acctttcgcg cgttcgttaa ctttgatgta agccagacct
     3241 ttcgcgccgt agattttaac gaagttaccg tattcgtcga tctgcttacg ggtcagcgat
     3301 gcgccacccg gaacacgtag agcggcaaca cggcctttcg gatcgttcgc cggccccgca
     3361 aatactgcaa actcaacaga tttcagcaga tcggcaacgt cggtcagttc catcgggtta
     3421 cgcagatccg gtttatcaga accataacgg cgttctgctt ccgcaaaggt cattaccggg
     3481 aaatcgccca gatccacgcc cttcacttcc agccacaaat gacgcaccag cgcttccatc
     3541 acttcacgca cttgcggcgc ggtcatgaaa gaagtttcca catcgatctg agtaaattca
     3601 ggctgacgat cagcgcgcag gtcttcgtca cggaagcatt taacgatctg atagtaacgg
     3661 tcaaaaccgg acatcatcag cagctgtttg aacaactgtg gggattgcgg cagtgcgtag
     3721 aatttacctt tgtgcacacg agaaggcacc aggtagtcac gcgcgccttc cggcgtggct
     3781 ttggtcagca tcggagtttc gatgtcgagg aagccgtggt catccataaa acggcgcacc
     3841 aggctggtga ttttagcgcg ggttttcagg cgctgagcca tttccggacg acgcaggtcg
     3901 aggtagcggt atttcagacg cgcttcttcg gtgttgacgt ggttagagtc aagcggcaga
     3961 acatctgcgc ggttgatgat agtcagcgag gacgccagca cttcgatttc gccggtcgcc
     4021 atatcgcggt taatattttt ttcgtcacgc gcacgtacgg tgcccgtgac ctgaatgcag
     4081 aactcattac gcagttcaga ggccagcttt aacgcgtccg cacgatccgg atcgaaaaat
     4141 acctgcacga taccttcgcg gtcgcgcata tcgatgaaga tcaggctacc aagatcacga
     4201 cgacggttga cccaaccaca cagagtcacc tgctgcccca cgtgggacaa acggagctgt
     4261 ccacaatatt ctgtacgcat gagatatccc ttaacttagc tgccggcgga tgccccctgc
     4321 tgcgcaggtg accaagtcgc agcgttagct gtatgtcaca actgaatgaa aaaaggcggc
     4381 tattatactg gaaattctgc cgcaccgtaa gagcctggcc cgcgctggaa cgcctcgtta
     4441 ccactttata tcgggcctga aatcagactc tacgccagtt tgctataaag gtgttgcccg
     4501 aactcataaa aattaacaaa atttgtcgtt ccgccatcgg ctaatcgcat taaggtgtga
     4561 ggcacgattt tgttttttca ggagtcatta tgcttgaact taatgctaaa accactgcgc
     4621 tggtggtgat cgatttacaa gaaggcatct taccttttgc cggtggtcca catactgccg
     4681 atgaggtagt taatcgcgcc gggaagctgg cggcgaaatt tcgcgccagc ggtcagcccg
     4741 tgtttctggt gcgcgttggc tggtctgccg attacgccga agcattaaaa cagccggttg
     4801 atgccccctc ccccgctaaa gtgttgcccg aaaactggtg gcaacatcca gctgcattag
     4861 gtgcaactga cagtgatatc gaaatcatca aacgtcagtg gggtgcgttt tacggtacgg
     4921 atctggagtt gcaactgcgc cgccgaggta tcgatacaat agtgttatgt gggatctcga
     4981 ccaatatcgg tgttgaatcc accgcccgca atgcctggga acttggcttt aatctggtga
     5041 ttgccgaaga cgcctgtagt gccgctagcg ccgagcagca caataacagc atcaaccata
     5101 tctacccacg catcgcccgt gtgcgtagcg tggaagagat ccttcacgcg ttatgattta
     5161 catcggtcta ccgcaatggt cgcatcctaa atgggtgcgg ttggggatca ccagccttga
     5221 agagtatgcc cgccacttta actgcgtgga gggcaacacc acgctttacg ccctgccgaa
     5281 acccgaggtt gtcctgcgct ggcgtgaaca gaccacagat gacttccgct tctgttttaa
     5341 gtttccggcg accatttcgc atcaggcagc attacgacat tgcgatgatt tagtgactga
     5401 atttttgacc cgcatgtcac cgttggctcc gcgcattggg caatactggc tgcaactgcc
     5461 tgccacattc ggcccacggg agctgcctgc gctttggcat tttctcgatt ctctccctgg
     5521 cgaatttaat tatggtgtgg aagtccgcca tccacagttt ttcgccaaag gggaagagga
     5581 acaaacgctt aatcgcggtt tacatcagcg cggcgttaat cgggtgattt tagacagccg
     5641 cccggttcat gcagcatgtc cacacagtga agctattcgc gacgctcaac gaaaaaaacc
     5701 taaagttccg gtacatgctg tactgacggc gacaaatccg ctgatccgtt ttatcggtag
     5761 tgatgatatg acgcaaaacc gggaattatt tcaggtctgg ttacagaaat tagcgcagtg
     5821 gcatcagacc actacgcctt atcttttttt acatacgcca gacattgccc aggccccgga
     5881 actggtacat accctgtggg aagacttacg taaaacgctt ccagagatcg gagcagttcc
     5941 ggctattcca cagcaatctt ctcttttctg aatttgccac ctatcataga caggtgccat
     6001 cggccatttt aaagggagtt tgtatggtaa gcgcgctata tgccgtttta agtgcgttgt
     6061 tattaatgaa gttctctttt gatgtcgttc gcttgcgttt gcagtaccgc gttgcctatg
     6121 gtgacggtgg ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat
     6181 atattcccat cgcgattgtg ctgatgctgt ttatggaaat gaatggcgca gaaacctgga
     6241 tggtgcatat ttgcggcatc gttttgcttg ctggtcgtct gatgcattat tacggttttc
     6301 atcaccgtct gtttcgctgg cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc
     6361 tgatggtgct ggcgaatctt tggtatatgc cctgggagtt ggttttctcc ctgcgttagc
     6421 gcacaatacg ccactttctt tttcccggat ttttacgtta tgtctcaccg cgacacgcta
     6481 ttttctgccc ctatcgccag actgggcgac tggacctttg atgaacgggt agctgaagtc
     6541 ttcccggata tgatccagcg ttccgttccc ggctattcca atattatttc catgattggt
     6601 atgttagccg agcgcttcgt tcaacctggt acgcaggttt acgatctggg ttgttctctg
     6661 ggcgcggcga cgctctcggt gcgtcgcaac attcatcatg ataattgcaa aattattgcc
     6721 atcgacaact ccccggcgat gattgaacgc tgccgtcgtc atattgacgc ctataaagcc
     6781 cctacgccag tagacgtcat tgaaggtgat attcgcgata tcgccattga aaacgcatcg
     6841 atggtggtgc tgaattttac cctgcaattc ctggaacctt ccgagcgcca ggcattactg
     6901 gataaaattt atcaaggact gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt
     6961 ttcgaagatg ccaaagttgg tgaactgttg ttcaacatgc accacgactt taaacgtgcc
     7021 aacggttaca gcgaactgga gatcagccag aagcgcagca tgctggaaaa cgtgatgctg
     7081 accgattccg tggaaaccca taaagcacgc ctgcataaag ccggttttga gcatagcgag
     7141 ctgtggttcc agtgctttaa ctttggttca ctggtggcat taaaagcaga ggacgctgca
     7201 tgatcgactt tggtaacttt tattctctga ttgccaaaaa tcatctttca cactggctcg
     7261 aaacgctgcc cgcgcagatt gctaactggc agcgcgagca gcagcacggg ctgtttaagc
     7321 agtggtccaa cgcggtggaa tttctgcctg aaattaaacc gtatcgtctg gatttattgc
     7381 atagcgtaac cgccgaaagc gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa
     7441 cgcttatgcg gaacctgatg ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca
     7501 tcgataccga atggcgttcc gactggaaat gggatcgtgt tctgccccat ctttctgatt
     7561 taaccggacg caccattctg gatgtcggct gcggcagcgg ttatcacatg tggcgcatga
     7621 ttggcgcagg cgcgcacctc gcggtgggga tcgaccctac gcaactgttt ctgtgccagt
     7681 ttgaagcggt gcgtaaacta ctgggtaacg atcagcgcgc acatttgtta ccgttaggta
     7741 ttgaacagct tccggcactg aaagcctttg ataccgtctt ttcgatgggc gtgctttacc
     7801 atcgccgttc gccgctggag catctctggc agttgaaaga tcaactggtg aatgagggtg
     7861 aactggtgct ggaaacgctg gttattgatg gcgatgagaa cacggtgctg gtaccaggcg
     7921 atcgttacgc gcaaatgcgt aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact
     7981 ggctgaagaa gtgtggtttt gtagatatcc gcgttgtgga tgtgtgcgtt accactactg
     8041 aagagcagcg acgcactgaa tggatggtta ccgaatctct gtccgatttc ctcgacccac
     8101 atgatccgag taagacagtt gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc
     8161 gcaagccgta aaggtctggc attactgccg gatgcggctt gaatgcctta tccggcctac
     8221 aaacccttgc taattcaatc tattgcaggg actatgtagg cctgataagc atagcgcatc
     8281 aggcagtttt acgtttgcat aacctcagcg cccgtttccg ggcgctattc acttcttacg
     8341 cctgaacagc aggcttatca aatgccgtta acttcggtgc gttacccgta tatttttcga
     8401 tatacaccag cgcagagtta ccggcacagg catttgccag ctgcgagctg gggatatccg
     8461 ccgttaacac gttcgcactg ccgtttttac acaagccgtt ttccagatct ggccatgcac
     8521 cttcatgcac gcataccacg ccttttttga tcccgtcagt caccaccgcg cctgtcaaaa
     8581 tctgaccacg tttgttccag acacgcacca gatcgccatt cgcaatacca aatcgagcag
     8641 catcttcggt gtgaatagtg atcggttcac gatcggcaac cgcatatttt ttacgcagtt
     8701 ccgcatagtt aagctgacta tgtaaacggt gtgccggatg tgcggtcaga agctgcaact
     8761 gcttctcgtc ggcggtaccc ttccactcat caggcgcaag ccaggttgga tgtgccgggc
     8821 aatccttata gccaaatttt tccagcgttt tggagtaaat ctcaattttg ccgcttggcg
     8881 tacccagcgc atttttcacc ggatcggcgc ggaaatcagc ataacgaacg tactgttcgt
     8941 tcttctcgct gcggcgcatt tcgatcagtt tattttgctg ccagaaggca ttaaacatcg
     9001 gcatagtcac gcgttgcgca cgagcacctt tctgagcggc atcatagaaa aatttcagcc
     9061 acgccatttc atctttacct tcggtataga tctcttttcc gccaggtttc agtaattcag
     9121 caagatcggc aaatacgtca aaatcgttac gcgcttcaaa ttgcggagca acagcctgct
     9181 tcatcggcac aatatgctgg ttgctgtaat caccggtcat cgtcaagtca ttgcgctcaa
     9241 acgatgtggt gatcggtaat acgatatctg catgtttagc agctgcggtc cagtagcatt
     9301 cagaaacgac gatcatctcc ggtttctgcc atgctttgat cagacgatta gtatcctggt
     9361 gatgggtaaa gttaccgcca cccgcccacc agatcatttt gatattcgga tacgtttgtt
     9421 ctttaccgtt atgctgatat ttgccaccag gattttccag cgcatcgacg atacgggcaa
     9481 caggaatcgc cgtcattccg ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg
     9541 cagacatttc cggcagcacg ccgccaacac gcgtcggatt gccaccgttg gagtaatgat
     9601 aagagaaacc aaaaccgccg cccggtgtac caatctgccc taacatcgcc gccagcgtca
     9661 ccagcatcca gtgtttctgt tcaccatatt gctggcgctg aattccccag ccagccatca
     9721 gcattgtgcg attcgccgcc atcagttcag cgagtttgac tatctgcgct tccggtacgc
     9781 cagtaatttc cgccgcccag gccgcgctct tcggagtatt atcgctctta cctgtcagat
     9841 actcttcaaa ctgcggatac ccggtggtgt atttttcaag gaacacttta tcgtgtttac
     9901 cttgtgtcat cagggtatgc gcaatcccta acatcagtgc cacgtcggtg cccatattcg
     9961 gcgcgatcca ggtggcatta tcaccaaaga actcgatggt ttcggagcgg ataggatcaa
    10021 tggcaatcac tggtttgcca gattttttca gctgatggaa gtattccagc ccttgctcat
    10081 cggtactgct ccaggcaatt tttaaggtat tcagcgggtt catcccccac agcaccacag
    10141 cctggctgtt ttccagaatc agcggccagg aggtctgctg ttcatacacc tctacagaac
    10201 cgaccacatg cggcatgatc acctgtgctg ccccggttga ataatcgccg ctatgcccgg
    10261 aataaccgcc cgccaggttc atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc
    10321 cagaagagcg ccagccgtat gatccggcaa aaatagcaga tggtccgtta gctttgcgaa
    10381 tgcgatcgtg ttgctcatga atcagtttta atgcttgctc ccagctcacc tgtacataag
    10441 tatcttcgcc acgacctttc gccggttgca gtggattatc gagatagctt tttcttacca
    10501 tcggatgctg aatacgtgcc gtggtgtgta cctgatccgc cgccgtagac tgtaaggagt
    10561 tcggtatggt tttcgccagc gcgcctgttg aagaaacaat cttgccgtcc ttcacttcta
    10621 cgttcatcgc tccccaacgt cccgcggtga ggattttacc gcccttctct tctgcccatg
    10681 cggggagcgg tgctgccgat gtcaccacca gcgctccagc ggcaataccg ctgtgtttaa
    10741 taaattcacg tcttgttaat gtcataactt cctccctgat caacgaggat cactgtttct
    10801 cggtaatatc tttggcgttg tactggaaat accgcgttaa aatgtccagt tcgttttcgc
    10861 tcatgctggt tcgcgcaccc attcctttgg caatggacgg ccatgcattg acggtgtaat
    10921 ggtcagcggc aataggcgca tggcaaccag cgcaataggt atcgtcaagt ttttcagcgt
    10981 attgccatag cggtttacgg tccgctaatg cgggatcggt aagcgcaccc tgtaaagacg
    11041 cctgacgcca ttgattgccg tattcgtcag cctgccattc cccgtttacc gtgagcgcct
    11101 tgataccttc ttcacttaat gtggctagcg ccagccgttg acctgccgcc aggtagagcg
    11161 tgttttcact gccctgcatt tgataaccct gcaacagaac gatcggctgt ttgccactgg
    11221 catcaacgac ggtgagatcg gtaccaggat tcacggtagc cagctcgcct atgcgagaag
    11281 ttttgaaagg ataaatatgt gcgccattag tcactgaagt agcggcctga ctttccagct
    11341 catgcgccgc gttgtcatcc atttttattt ctggcggaaa atgggcaatg cctttatgac
    11401 aatcgataca ggtttcgccg cccttttgtg ctttgttatg cattttctgc gcagattcac
    11461 tttgcgaggc aatatccatg gcatcaaaag aatggcaact acggcacgtt gcagagtcag
    11521 ttgcttttaa ttctttccat actgtttcgg ccatttcctg gcgatgagtt tcgaacttat
    11581 cgtcactgtc tattttgccg ctaacaaatt catgataaat atctttagat gcttttaatt
    11641 tagcaaataa ataatccatc cctgactttg gaatatggca atctgcacat tccgcacgta
    11701 tccctttctg gttcgaaaag tggacagttc cctgatattc ctcaaaaggt ttactcatcg
    11761 agtggcagga aaggcaaaat gctgtatccg acgttttatg taaggctttt tgcgccagca
    11821 ataacccgcc gccaccaacc acaaccgcta tcagcaaaaa caataaccca atgcgttttt
    11881 tccctcgcat agattcctct aaataaattc cagaacaccg aaatcattgt ataattaaat
    11941 acacaacgaa ttaaccgctt gcataactcg gcacaacact gcctgaaata atcgataaag
    12001 aatatgattt attacaatgt aatcattaat tgctaaggaa taacccagtt gctatttaga
    12061 atatatattt atcagtttta gcaatttaaa tcccataatc aatgtgaata taaaaaatat
    12121 tattagcaca acgctatgtt cactgtcacc cgctcaacag atcacggtta tcgttcgttt
    12181 tttatactgc tcagggataa aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc
    12241 aagcatcata ttgggcgaca tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa
    12301 tgattccttt catttcagca accgccgccc cgtctacgat ataacgcgaa tactcgtccg
    12361 cgtgttcatc tgatgacatg gacaatcctt gattacgata acgcatcggt gaggcttgcc
    12421 acgctcccgc ggagctatgg acttccagca ctccggcatc gaggaagtgg tgcaggtttt
    12481 ctgcacggac tcctgctccg gccataatga ttggagcatc acgatgggca ataagttcca
    12541 taatttttga taaaccttgc agtgcgtctg atttttgccc tgatgtcagt acccgggcaa
    12601 tgcccaattc cgcaagatta ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc
    12661 gatgaaaagt cactgccagc ggaccggcag cagccattat tttttccatt cgtggcatat
    12721 cgacattccc gtcaacatcg agaacgcccg tcaccagtcc aggaaaacct aattcgcgga
    12781 ccgtgcgcac atcctcaaga atggcggcaa actcaccgtc gctgtagcaa aaatcaccac
    12841 cgcgtgggcg aattatcgga tgcacaggga tcgtcacccg ctggcgcacg gatttcagta
    12901 cccccaacga cggcgttaag cccccctctt ttggggctgc gcataattca actctgtctg
    12961 cgccgttttg ctgcgccgtt agtgcacatt ccatgctgta acagcaaatt tccagtaatg
    13021 ccatttttgc tccttaatta cgctgactgc tcgctggcaa cgatctcttc aatggaccac
    13081 ggatgaaact taatagttgt tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc
    13141 tcatgctcac cttttggtga actggtttta actgagatcc ccggtaggct gagtccttca
    13201 tcagaaagca atgccagcgc acgggcattc agcgtttctg gatcacccgg cagaacgatt
    13261 tcaatatgtt cccaaccttc gtgtgggtaa cgtttttccc cgggccacgg tagctccaca
    13321 atagaaaact gccagtgcgc aacctgtacc ggttcatgca atttaaacag acaaatcggt
    13381 ctgccattga tcatattttc tgacaaaagc tcgccacact gttcaaaccc gcgacgccag
    13441 cgttcagcag tggcgttttg atggcaacgc aaagaaatgt gatcggcagt cagcggagtg
    13501 atattcaacc ccagacggag ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc
    13561 gatgcaatat cctgcagctc gtcgatagat tgccagttcg ccataatcac tcttcgtctt
    13621 tcagtaaaag cgttaattta ccctgttgcc ctgcgccaac caaccgctga tttcacgccg
    13681 cttctgatgc aatagtgaaa acggcaatac gccacgcgca cgttgctgac gaaaacagcc
    13741 atttgcagta tactcccgcc ctaatttctt taactggtgc gggcaatttt tgctcgcttc
    13801 atcaatgtaa ggtattccgg tgaatattca ggctcttctc tcagaaaaag tccgtcaggc
    13861 catgattgcg gcaggcgcgc ctgcggattg cgaaccgcag gttcgtcagt cagcaaaagt
    13921 tcagttcggc gactatcagg ctaacggcat gatggcagtt gctaaaaaac tgggtatggc
    13981 accgcgacaa ttagcagagc aggtgctgac tcatctggat cttaacggta tcgccagcaa
    14041 agttgagatc gccggtccag gctttatcaa cattttcctt gatccggcat tcctggctga
    14101 acatgttcag caggcgctgg cgtccgatcg tctcggtgtt tctacgccag aaaaacagac
    14161 cattgtggtt gactactctg cgccaaacgt ggcgaaagag atgcatgtcg gtcacctgcg
    14221 ctctaccatt attggtgacg cagcagtgcg tactctggag ttcctcggtc acaaagtgat
    14281 tcgcgcaaac cacgtcggcg actggggcac tcagttcggt atgctgattg catggctgga
    14341 aaagcagcag caggaaaacg ccggtgaaat ggagctggct gaccttgaag gtttctaccg
    14401 cgatgcgaaa aagcattacg acgaagatga agagttcgcc gagcgtgcac gtaactacgt
    14461 ggtaaaactg caaagcggtg acgaatattt ccgcgagatg tggcgcaaac tggtcgacat
    14521 caccatgacg cagaaccaga tcacttacga tcgtctcaac gtgacgctga cccgtgatga
    14581 cgtgatgggc gaaagcctct acaacccaat gctgccagga attgtggcgg atctcaaagc
    14641 caaaggtctg gcagtagaaa gcgaaggggc gaccgtcgta ttccttgatg agtttaaaaa
    14701 caaggaaggc gaaccgatgg gcgtgatcat tcagaagaaa gatggcggct atctctacac
    14761 caccactgat atcgcctgtg cgaaatatcg ttatgaatca ctgcatgccg atcgcgtgct
    14821 gtattacatc gactcccgtc agcatcaaca cctaatgcag gcatgggcga tcgtccgtaa
    14881 agcaggctat gtaccggaat ccgtaccgct ggaacaccac atgttcggca tgatgctggg
    14941 taaagacggc aaaccgttca aaacccgcgc gggtggtaca gtgaaactgg ccgatctgct
    15001 ggatgaagcc ctggaacgtg cacgccgtct ggtggcagaa aagaacccgg atatgccagc
    15061 tgacgagctg gaaaaactgg ctaacgcggt tggtattggt gcggtgaaat atgcggatct
    15121 ctccaaaaac cgcaccacgg actacatctt cgactgggac aacatgctgg cgtttgaggg
    15181 taataccgcg ccatacatgc agtatgctta cacgcgtgta ttgtccgtgt tccgtaaggc
    15241 agaaattaac gaagagcaac tggctgcagc tccggtaatc atccgtgaag atcgtgaagc
    15301 gcaactggca gctcgcctgc tgcagtttga agaaaccctc accgtggttg cccgtgaagg
    15361 cacgccgcat gtgatgtgtg cttacctgta cgatctggct ggtctgttct ctggcttcta
    15421 cgagcactgc ccgatcctca gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact
    15481 ggcacaactg acagcgaaga cgctaaagct gggtctggat acgctgggta ttgagacagt
    15541 agagcgtatg taatcgattt ttcgtgagag taaagcctga tcagggttac ctgatcaggc
    15601 ttttttattg ccatctaaat gtattctgaa aatggacatg ccattgtttt ctgactgttg
    15661 gataagaggc cagaagcgta atatccggcc ccagggaaac gataacggtt gaatttaagg
    15721 aataccgcag tgtttaaatt tcttgtatta acattaggca ttatctcttg ccaggcttac
    15781 gcagaagata cggttatagt aaacgaccat gacatttcag ccatcaaaga ttgttggcaa
    15841 aaaaattcag atgatgatac tgacattaac gtgatcaaat catgcctgcg acaagaatac
    15901 aatctcgtcg atgcgcaatt aaataaagcc tatggtgaag cttatcgtta tatagaacaa
    15961 gtgccacgca caggtgtaaa aaaacctgat accgaacaac ttaacttgct taaaaaatca
    16021 cagcgagcct ggctggattt tagggacaaa gaatgtgaat taatcctttc aaatgaggac
    16081 gttcaggatt taagtgaccc ttattctgaa tcagaatggc tctcatgtat gatcatacag
    16141 accaatacgc gaactcgcca gttgcagcta taccgtaact ctgaagattt ttatccaagc
    16201 cctttgacaa gaggataatt cacatctttt tggcatgttt tgttgcaagc tattcctgat
    16261 aaataattgc aacaagacat cgagcctttt tcactgagtt attaaacata ctcgcgagcg
    16321 cgtaattttt ttgtccttca gcgataattc acaatcactt cattacgttg tacctttagc
    16381 ggtggaatta accgcccacc gcctggcact tcccagataa atcgcaacgg ttctgctgcc
    16441 gctataccgg aaaaggccac ggtggttccg ctctgcccct ctaattcgac acaacgagat
    16501 tgcgaacaca agcgaacccg cagtcctgaa ggtgtcgggc cgataagctg ataacgccac
    16561 gctaccagcg tcatcaatcc tgaagcaggt tgtcgcgtag aaagaggcgc agacgacatc
    16621 gactcaccgc gatgatttag cgtaattcct acactacttg cctgccacat cccctccccg
    16681 gcggcttgca ccagcagcgg aaacaataat attgctaata aggttctcat tatttgccgc
    16741 caattgtcgc cgtcatgcgg atatgtcggt tatcagacag ttccagattc gacaggacca
    16801 ctaactgcgg caagctgcgg cgcaggaagc gagacaataa tggtcgcggc gcgtggttca
    16861 ccaacaatac tggcggcgca cccagcatct cctgacggga tagcgcttcc tgagtttgcg
    16921 ccagtaaacg atccgccagc cctggctcca gtcctccccc gccctgcagc gcctgtagta
    16981 acaaacgttc cagcggtgta tcgaggccaa taacatggac ttcatctttg ccaggaaacc
    17041 actgctgggt aatcgcccgt cccaacgcca cgcgcacgac ggcggttaat tcatgtggat
    17101 cgctttggat gggcgcatgt tccgccagcg tttcgagaat ggtgcgcata tcgcgaatcg
    17161 gtactttttc atcgaggaga ttttgcagca ctttatgcag tgtggtgagc gtgacgacgc
    17221 caggaacgag atcttccgtc agctttggca tctcctgggc gacgcgatcc aacagctgtt
    17281 gcgcctcctg acgaccaaac agctctgcgg catgctggct aatgaggtgg ttaagatgcg
    17341 ttgctaccac cgtgctggcc tcaaccactg tgtacccctg aatctgcgcc tgttctttta
    17401 gcgcactttc aatccagata gcattcaggc caaatgccgg atcgacggtc gcctcaccag
    17461 gtaacgtccc ggcagcggtt ccagggttaa tcgccagcca gcgccccgga taagcatcac
    17521 cactgccaat ctccacgcct ttcatcaaaa tgcgatagcg ggcaggttgc agctccatat
    17581 tgtcgcgaat gtgcaccact ggcggcagaa atcccatctc ctgggcaaat ttcttgcgga
    17641 tactgcgtat acggcccaac aactcaccat cctgctggaa atcgaccatc gggatcagtc
    17701 gataacccac ttccattccc agagaatctt ccagttgtac atcgttccac gtcgcttcga
    17761 caacggtatt attctctgcc atttttaccg gtttgggttc gacaggcgct ttttgttcgc
    17821 gtccgcgtat ccaccaggcc agccccagca atccggcagt gaacagcaaa aataccaggt
    17881 tcggcattcc aggcaccagg ccgagtaaac cgagcacggc ggcgcttaac aacataacgc
    17941 ttgggttact gaaaagctga ttcaccatct gctcgccaac atcctgatcg gtgctgacac
    18001 gcgtaacgat gacccccgcg gcggtagaaa tcaccagcgc cggaatttgt gccaccagac
    18061 cgtcgccaat ggtcaatagc gtataacttt ccgccgcgtg tcccatgctc atgccatgtt
    18121 gcagcacgcc gaccagcaac ccgccgacaa cgttaatgac catgatgagg atcccggcga
    18181 tggcatcgcc gcgaacaaac ttacttgccc cgtccattga gccgtaaaaa tcggcttcct
    18241 gagtcacttc ggagcggcgt tttttcgcct catcttcacc aatcaatcca gcgttaaggt
    18301 cggcgtcaat cgccatctgc ttacccggca taccatcgag aacaaagcgc gcacccactt
    18361 ctgcgatacg cccggcacct ttggtaatga ccataaagtt gatgatcacg agaatgacaa
    18421 acactacgat accgatagcg aaattgccac caacgaggaa gtgaccgaat gcttcgacca
    18481 ccttcccagc cgccgccgcg ccggtatgcc cttccatcaa aatgatacgg gttgaagcca
    18541 cgttaagtgc cagacgcaac aacgtagtaa acaacagaat ggtcggaaac gcagcaaact
    18601 caagcgtgcg ctgggtaaac atcgccacca gcaacaccat gatcgacaag gcaatattga
    18661 aggtaaacaa caggtcgagt atgaatgcgg gcagtggcag caccatcatc gacaagatca
    18721 acaggatcaa aatcggtccg gcaaggatct gccattgtgt cgatttcagg tttgcgggca
    18781 ggcgcagcat cgcggccaga ttactcatgg gtcggtttct cgttaataaa atccagggct
    18841 tccggcaccg gaagatgagt aggttgtaca gggcgctgtc caccagccag acgccagcgt
    18901 ttcagttgcc agacccaggc cagcacttcc gccaccgcgg cgtacagttg acccgggatt
    18961 tgttgaccaa tctccgcatg tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc
    19021 gggacgttat tttcagcgcc aatttcacga atgcgcagcg cgaccagccc tgcaccttta
    19081 gcgaccactt tcggtgcgct cattttgttt tcgtcatact gcaacgctac cgaatagtgg
    19141 gtcgggttat tgacaatgac atccgctttc ggcacatcgg ccatcatccg acgccgtgca
    19201 gcagctcgct gcatctgacg gatccgccct ttaacatgag ggtcaccttc gctttgtttg
    19261 aactcatcac gaatatcctg ccgtgacata cgcagctttt tcaggtggct gaatatttgg
    19321 aaaaagacgt caaatcccac cattggaatg acaccaagca ccaccagcag tgcgcatagc
    19381 cctaccaaat ccatcgcatt acccatggcg gtaatcggag actcggccat caagcgcatc
    19441 atctgcggcc agtgatgcca gagaaaaaac cccgtcacgc tgccaaccag gatggttttc
    19501 aaaattgctt taagcaactc cgcgccagtc tgagccgaga acatccgttt aatgcccggt
    19561 agcgggttga gtttggaaaa cttcggctgc aaggatttgc cgctaaatac cagccctccc
    19621 agcatgaccg gagaaatgag cgccaccagc accacgccgc taatcagcgg cagcagcgcc
    19681 agcatggctt ctctgatcag cagaataatc tgcccgagga tcagattcgg gtcattgata
    19741 atactgtgat caaaatgcag cccagcggag agcatgcccg acaatcgacg ggccagcgac
    19801 acaccgccaa accagataac actaacgccc accagcaaaa tcagcagtga ggtcagttca
    19861 cgggaacgcg ggatttgccc ctcttcccgc gctttttcta gtcggtgagg tgtgggggct
    19921 tctgttttgt cgtcgctctc gtcagacacg tcgccaatcc tggaaagagt attaagcgtg
    19981 aatgatgcca gagcgcaaag cgttcaatgg tttgagtaag gggcaaaaca ggcgggattt
    20041 agggcttttg ctgccacaca tcaagcatag tgtgcgtttg tcggatgcgg cgtcatcgcc
    20101 ttatcagacc gcctgatatg acgtggtcac gccacatcag gcaatacaaa tcaaaatcca
    20161 agactatcca acaaatcgtc cacctgatcc tgactggcta ccacaccggc tttgctggta
    20221 tcgacctgag gtccattaag caaactctgg ttttcacgtt ttggacgcga ctcctgttcc
    20281 gggatgtttt ccaacagcac catcagcaac tggcgttcga tctcctgaat gacatccatc
    20341 atccgcttaa tgacctgccc ggtgagatcc tgaaaatcct gcgccatcat gatttccagc
    20401 agttgcgcgt tagtaaagct ggtatgcgcg ggtacatctg ccagaaattg tcgtgtatct
    20461 gttaccagtt cacgggcgtc ggcaaggtca atcggatcgg caaaccagtc atcccaacgt
    20521 tgggttaacg cttttgctga tttctccatt tgatcctgat gcggttgtga cgcctcaaca
    20581 ctgttcagcg cccgctccgc agcctgggcg gtcatctgca caacatagta caaacgatcg
    20641 cgcgcatcgg ggatggcttc cgccgcttcg gcaatggcct gatccagccc cagttcccgc
    20701 aaactgtcgc gcagcatacg cgtcaggctg ccgatgcgcg caatgatatc gccagctgaa
    20761 tgctcgtcag caggtttgat tgatggttgc atcatagtcg catcctcaca tgcccagttt
    20821 ctcaaagatt ttgttgagtt tttcctccag cgtcgcggcg gtaaatggct tcaccacata
    20881 gccactggcc cccgcttgcg ccgcagcaat gatgttctct ttcttcgctt ctgcagtcac
    20941 cattaacact ggcaatgccg acatcgcgcc atccgcacga attgttttca gcaattccag
    21001 gccatccata ttgggcatgt tccagtcgga gataacaaat ccataaccgc ctgcctgcaa
    21061 cttattgaga gcgtcgacgc catcttccgc ttcctcaaca ttattgaatc ccagctcttt
    21121 cagcaggtta cgcactatgc gtcgcatggt ggaaaagtca tccacaacca aaaatttaag
    21181 ttctttatcc gccatttcac actcctgatt taaatacgta tcgcctgtcc ggcactaatt
    21241 tttgccaaca tttgctggct tacctggcta agatcgacca cttcgcagac accacccata
    21301 ttgatggcct cgcgcggcat gccgaacacc acgcaacttg cttcgttttg cgcaagggtc
    21361 catgcccccg cctgacgcat cgccaacatt cccgccgcgc cgtcgttgcc cataccggtc
    21421 aggatcaccc caaccgcatt acgccccgcc tgtttggcga cagaatggaa caacacatct
    21481 accgaaggcc gatgacggtt aaccgccggg ccatcgtgaa ttttgatttg gtaatttgcg
    21541 ccactacgcg acagctccat atgccgatcg cccggcgcaa tataggcatg ccccggcaag
    21601 acacgttctc cgtcttcggc ttctttaacc ccgatctggc aaagcttatt aagtctgtcg
    21661 gcaaaagagc gggtgaaacc gggcggcata tgttgggtaa ttaacaatgc cgggctggaa
    21721 agcggcaacg gttgcagtac gtgacgaatt gcctcagttc cacccgttga agcaccaatc
    21781 gcaatcagtt tttcagaact caacaacgga cccgccttca gcgttgtcgg tgctgacaat
    21841 ggcttatgtg ctgcaaggct cgcctttgct gccgtacgca ccttttcagc aatcatttcg
    21901 ctatacgcca gcattccttc gcgaataccc agttgcggtt tggtgacaaa atctattgcc
    21961 cccagctcca gcgcgcgcag cgtgacttct gatcctttgc cggtcaagga agaaaccatc
    22021 acaacgggca ttggacgcaa acgcattaat ttttcgagga aatccagtcc gtccatccgc
    22081 ggcatttcaa catccagcgt cagcacatcg ggattgaatt tcttaatcaa gtcacgcgcg
    22141 accagcggat caggcgcggt cgccaccatt tccatgtcgc tatggctgtt gatgatttct
    22201 gtcatgatct ggcgcatcag tgccgaatca tcgacagata acaccctgat tttgctcatc
    22261 gttaatcctt acttagcgca tacaccgtct gaccacgcag cgtgaagcgg cgctcaaggt
    22321 ggctaaagtt ttcagagtga cccgcaaaca gcaatccgtc gggtttaagg agcggaacaa
    22381 agcggcgcaa aatctcctgc tgggtatttt gatcgaagta gatcatgacg ttacgacaga
    22441 agatcgcatc aaacggcccc ggcacggtgt actgtttcgc cagtagattc agcggggcaa
    22501 aatcaacata gttcgccagc tcctgacgca cgcgtaccag cccttcatgc ggtcccgtcc
    22561 ctcgcatgaa ataccgttgc agttgctgcg gcgtcaggtt tttcaactct tcatggcgat
    22621 aaataccgct tctggctttt tccagcactt cggtgtcgat atcactggca aacactttcc
    22681 agcgtccggg cgcggtgccc aatgtgtcag ccagcgtcat cgcaatgctg tacggctctt
    22741 cgccggtcga agccgccgcg ctccatacgc gatactcgcc agaacggcga cgtgcgtgat
    22801 ccgcgagcag agggaaatgg tgtgcctcac ggaaaaatgc cgtcagattc gtggtcagcg
    22861 aattgataaa cgcctgccac tcaccgctgt gctgattaga ttccagcaag ttcagataat
    22921 gaccgaaatc cgtcagtccc agcgaacgca aacgacgaac cagtcggttg taaaccatgt
    22981 cgcgtttatg gtcagccaga acgatcccgg ctcgttgata gatcaattga cttatccgcc
    23041 gaaaatgcgc gtcggaaagc gccaggcgct cggtcatctg taacaataaa gacgtttgcc
    23101 cacagggcag agatgaagtc atagcgcctt ctcaatcact tcaggatacc actggcgcaa
    23161 tttgtaactg cgccgactca tgtcgtgcta cttcatgttc ttcaagggta aataccgcca
    23221 cacgcgacga aagatggtcg gcctgattcg ccagttgttc cgttgccacc gccgcttctt
    23281 ctaccagcga ggcgttctgc tgagtcacct gatccatctg gctgacagcc tgtgcaacct
    23341 gctcaatccc ccgctgttgt tcttccgacg cagaggcaat ttctcccata atgtcgtcca
    23401 cgcgggtcac cgaactgaca atatcgatca tggtcgcggc ggcgttattc accagtttcg
    23461 aaccctgctg gacacgattg actgactctt cgatcagccc tttgatctct tttgctgcct
    23521 gcgcgctacg gctggcaaga ttgcgcactt cacctgccac taccgcaaaa ccacgcccct
    23581 gctctccggc gcgagccgct tccactgccg cattcagggc cagaatattg gtctggaaag
    23641 caattccgtc gataacgctg ataatgtcgc caattttttg cgagctggtg gcgatctcct
    23701 gcatggtgtg agtcatggta ctgacctgaa caccgcccgc ctgcgccgtt gtcgcggcat
    23761 tttttgccag ttccgacgcc tgtcgtgcgt tatcggcgtt ttgccctacc gtggcggtta
    23821 attgctccat actggcggcc gtttgtgcca gcgatgccgc ctgctgttcg gtacgacttg
    23881 agagatcgtt attgcctgcg acaatctccg cgataccaat gtgcatctcc tggcttccct
    23941 tacgcacatc actcaccgtc ccacgcaaag cctgctgcat ggtcttcagg ctggcaaaaa
    24001 tggcggtgat ctcattacga ccatataccg caatcggacg cgccagatta cccgcagcaa
    24061 tgctgtcaaa atggctaccg ataatggcca gtggttgaac aatcatcttg cgcgtccacc
    24121 acagcgcact gctgatgtag atcgccgcaa caataatcat gctgataaac accagcgccg
    24181 aaatctgata gttacgctgg ctttgcgcac tggcggcttc cagcacatgg ttgatctcca
    24241 gctgccaggc ctcaaagtta acgtcaaacg cattctgtga ttcctgcacc ggcgcagtga
    24301 ggaaatccga aagttgatta ctttcaagcc aggtcgcctg atgttcgaga tcgttgttgt
    24361 gccagcgggc aaaacttttc tccgtctctt tttgcaacgc cctgacgtgc tcgttgcccg
    24421 cagtcatcgc cataaaactt ttaaacagcg tggtggattg cgtcagactg gcgcgcgccg
    24481 tcgtcatcaa cgttttaatg tcatccgccg gatagctaag tgccgtcaga gtgcccgctt
    24541 tgttcagcgc ggtactggcc tgtaacatta ccgcccgagt ttgcgctaat gccgcacgtt
    24601 gctgattgct ctgctcgacc tgattcagtc gttgcaaatc gtcgcgaaat gcccaaaaag
    24661 acatgccgtt actgccaatc tgcaagatcc cgcagagaat caaaattaaa aacagcgtgg
    24721 tcgaaattcg aatacgatta aacatcaacg ctccccatca ggcggcaatg accgcgttag
    24781 taaatactcg tcaaaatgtt tcccagtttg gagcttgttc agcaattcgc agtcgtggct
    24841 gtgccggtgg ttgctcactg gcaggacggg atggtgtttg cggtttattg gtgagtgggc
    24901 tggctgccag acggaacgcg gaaaccgctt gcgttaaacg actcgcctgt tcttccagcg
    24961 cagcggcggc ggcagctgat tcctgcacca gcgatgcgtt ctgttgcgtg acgcgatcca
    25021 tttccgaaac cgccaatgcg acttgatcga tgccacggct ctgttcatcc gatgccgatg
    25081 caatctcgcc cataatgtca gtcacgcgag tgacagcatt gacgatattg ttcattgttt
    25141 ccccggcgct ttcgaccagc accgaaccgg tatcaacgcg tgagacggag tcttcaatga
    25201 gggctttgat ctcttttgcc gcctgggcgc tgcgactggc aagattacgc acttcacccg
    25261 ccaccacggc aaaaccacgg ccctgttcac ccgcacgcgc ggcttcaacc gcggcattca
    25321 gcgcgaggat attagtctgg aaggcaatac cgtcgataac gctgataatg tcggcaattt
    25381 tcttcgaact atcggcgatc tcatgcatcg ttttcactac gccatccacc actttgccgc
    25441 cgtgctgggc ggtgtcggag gcactttgcg ccagttgcga ggcctggcgg gcgttatcgg
    25501 cgttttgctt cactgtcgcg gtgagctgct ccatgctggc ggcagtttct tccagcgcgg
    25561 atgcctgctg ttcagtacgg gaggaaagat cggtgttgcc cgccgcaatt tcacgggtac
    25621 cggcatagat ggcatctgaa ccttcgcgga catgagtgac ggtgtcagtc aaagagcgtt
    25681 gcatatgtga aacgctctgc gccaggtcgc ccatttcact gcgcccgtca atggtcaggg
    25741 tattcgccag gttaccaccg gcgatttcgc gaatgtgagc aataattttt gccagcggag
    25801 taagcaacat acggcgaatg ccgtaccacg ccaccagcag aatcaatacc accaccagcg
    25861 cgataaccgc cagttgccac tgggcaaatc ggtaatcatc tgcgttgtca gtgacgatat
    25921 cgcgatacag tttttcactg ctgagggcgt actgagcaaa cgcttcgccc attgcatttt
    25981 gcattccctg ggttggctga gcgaaataag ctccagtatt gccataatca agataatcaa
    26041 tcagttcagt taacgctgtg tgatagtttt tatatttttc atcaatatta cgactggtag
    26101 cgaccatttc aggtaacggt gccatgcttt tgaatttttt ataatgagtc gcggcctggg
    26161 ccaatgtttt cctggcgcta tcgagcaatt caactttggc gttactttgt tgattggagg
    26221 aatccatcat catccgcacc gctgaacgac tcaggttaat gcgcgtttgc agcattaaat
    26281 cccaggttga cgtcagctcg ccctgctgtt cccgtaattg attggaaacc acaaagctct
    26341 tctggctatg gtgaagggaa gaaaaaaaca gactgccgga aataagctgt aacagtgcga
    26401 atacccccag caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc
    26461 ttcctgaaaa caagttaatc tcgttatcgg caagaagggg ggaaacttta ttgctgatgc
    26521 cacccgccgc gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt
    26581 gaggaaatcg ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt
    26641 cgctgttcag cagtttttcg atgttcacca gaatcaacat ccggtcgccc agtgcgccca
    26701 gtccagtgag atattctgtt gaaagcgtca cggcaaactc tggtgccgga cgaatttgct
    26761 ccgccgtcaa tgaaagcacg tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc
    26821 cgagattcag gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact
    26881 taattcgtaa gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa
    26941 acgctggcgt gttcgcaatc cgtgttacct gatcgtagcc acggatctcc tgcactttca
    27001 ggatatcaat accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg
    27061 gctcgctggc cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat
    27121 tcaggcggcg gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc
    27181 aacaatcagt gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt
    27241 gcgatagtta ctttcaaggt ttttaaccac aacctggtgc tgaccaatta attgatccac
    27301 cagcaaggcg tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc
    27361 ggttttcgcg cccgcgacgt tgaacacttt ccacagttcg acgatgggca gatattcacc
    27421 ccgcacttcc agcacccgct cgccgccggc cagtggatgc agatcggctt cacggggttg
    27481 cagtgattcc ataacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga
    27541 catgccgtcg aggatggcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc
    27601 ctgcttcgac tggatttcga catgaccgcc catctcctgg atattacgtt taacgacgtc
    27661 catgccgacg ccgcgcccgg agacgtcggt gacctgctct gccgtggaga agcctggtgc
    27721 aaatatcagc atcgcgactt cgtcgtcgct catgttttcg ctgacagtca aaccttgcga
    27781 ggccgctttt gccagaattc gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat
    27841 gcaaatgttg ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc
    27901 tgcggcgagc cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg
    27961 ggtcagcggg tcgataatgc gttctatcag gcttttgtcg agttcagtag aactgcccac
    28021 cagcgtcagt tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata
    28081 gcgactaaaa acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc
    28141 acgggcgtta cgttgtaact gccccatgct ggttatcaaa tcaccatgat taaccgggtc
    28201 cagttcgctg gaacgctggg caagcatgga ctgggtgata accagctcgc cgacgaggtt
    28261 aattaattga tcaacctttt ctaccgctac acggatgctg gtggattcat tgctgcgcgt
    28321 cgttttttcc cgctccacgc ggccggttgg cgcttgttcg gctgccagtt taagcactgg
    28381 tggggtggat atttttggcg agacttctac tgtttcaaag gtaatctgat cggcttcaat
    28441 cacaaaacag agtaccgctg tgatgtcatc ttcggcgatg tcgcccggta atattgccga
    28501 gagcgaatcc gcccctttca ccacgtcagt taacgttgtc agatgtccca gttcttcttc
    28561 cagcaggtcg acttccccgg ccttcaggcg cgaaaggata attcgtcgcg gcgactgact
    28621 gcgactctgc tcatcttgcg gttcactttt ggcaaccaca cttaatcggg tcactgcgga
    28681 tggcgtttcg cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgaa
    28741 gctggcggca tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc
    28801 cttcgtttcc aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc
    28861 ttcatcgagc aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt
    28921 tcctgcccct cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc
    28981 tggcgcttcc ggctgcaaaa ccagcagatg ctgctccatg tcagccaaca gttcgtccgc
    29041 ttcatcaaaa aatgtctgat aaaaatcgct tatatccatg ctcacgctgt cacctcggtt
    29101 cggctgatgg cattgtggga acactgacct gtggtgcaac ctcaggtttt tccagggcgc
    29161 ttactggctc attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt
    29221 tcagtaccag caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc
    29281 gcatcgttgc cgccatgccg acgacacgta acactttgcc gctatccaac cctccgacca
    29341 tcagttcgcg gcgggatgca ttggcccgat cggcagaaag ctcccagttg ctatatcctt
    29401 tctcaccgct ggcgtagggg aaatcatcgg tatgacctga aaggctaata cggttgggaa
    29461 taccgttcag tacaggcgca atggcacgca gaatgtcgcg catatagggt tcgacatcgg
    29521 cactgccggt tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt
    29581 cctggaccag atcgattttg agatggggac gtaacgcccg cagtttcgga tcggactcta
    29641 tcaactggtc gagatcaccc cgcaatttcc gcaatcgact ttgctccatg cgttttttca
    29701 gctcttcgat gttcggctgc ttattcactt ccccctggct ttgggtgtaa tcatcaccac
    29761 cgccgggaat tgggctttca ctattagaaa tgcgatcgcc gcccgtaacc gcagtcgcca
    29821 gtggagtccg gaagtactcc gcaatctgaa tcagctcttt tgggctggag atggagatca
    29881 gccacatcac cagaaaaaag gccatcatcg cagtcataaa gtcggcataa gcaatcttcc
    29941 acgatccatg tgctgccccg tggcttttgg ctttgcgtcg tttgacgaca ataatcggat
    30001 gcgcttgatt cttcatgctt cctcggttgt catctgttgt tgcggatttt tcaccgcacg
    30061 cacatgctct tccagttcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc
    30121 aaactcaacg gcgataggcg gtgcgtaacc gttcagatta gaaagcagag tgactttgac
    30181 gcactgcatc attttgctgg tttcggcgct tttctgacgt aaaacactcg ctaatgggga
    30241 aataaatccg taagccaata aaatgccgag gaaagtcccc accatcgcat gtgcgataag
    30301 cgcacccagc tcggcggcag gacgatcggc tgaacctaac gcgtgaacga cccccattac
    30361 agccgcgaca ataccaaacg ccggaagtga gtccccgacc agcgccagac tgttcgccgg
    30421 gacttctgcc tcgctttcgt gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa
    30481 ggtgttcata tgaccgctga taatcagacg cagataatcg acgataaaat caagcatgac
    30541 gctatccgcg aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat
    30601 atcacgttcc agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag
    30661 agccagcaga tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag
    30721 cgccttcagc gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc
    30781 tgcaccggca ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt
    30841 catcaaataa ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac
    30901 gacatcatcc ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg
    30961 attggggcaa aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgcctcaccg
    31021 tatcagttaa acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg
    31081 attctgggaa agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc
    31141 aaagctgcca acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct
    31201 ggaaagttgc agtaatccac tttcaacaaa ccgcaccaat gtccaggcac gggttaatgc
    31261 cagcagtggt ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat
    31321 caccgcatcg acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa
    31381 catcgaagca tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat
    31441 gcctttcggc ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact
    31501 taactgtgtt tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc
    31561 cagctgaata tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc
    31621 ttgcgcagcg cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg
    31681 ccggtatgaa tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc
    31741 tggtggctgt caaaacggaa gtgacaaacc agttgattgg tttctgccag cttaaccatt
    31801 tgcggaagag tcagtgccgc taacgttgtc gccatttctt catttatgcc gagacgaaac
    31861 atagcggacg ctttgtcctg aacaatcaaa cgctgtgcaa gtagtaaata tgacaagttg
    31921 atgtcataaa tgtgtttcag caactcggag gtatgcatta ttcccaccca gaataaccaa
    31981 ctttattttt atgcggtttc accgcacccc gtgatgtcgc cgggaagccc cggtaaaaaa
    32041 taattagcat tagaatagtt gcgataagct gcaataagca gaaccacctt tttggtttaa
    32101 tatgtcctta caaatagaaa tgggtcttta cacttatcta agatttttcc taaatcgacg
    32161 caactgtact cgtcactaca cgcacataca acggaggggg gctgcgattt tcaataatgt
    32221 gtgatgcaga tcacacaaaa
//
LOCUS       DAZFJF010000043        32125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFJF010000043 DAZFJF010000000
VERSION     DAZFJF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.043"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..295
                     /locus_tag="ACHKAU_002212"
     CDS             <1..295
                     /locus_tag="ACHKAU_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531274.1"
                     /translation="NGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTTTL
                     NLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(342..1229)
                     /gene="rarD"
                     /locus_tag="ACHKAU_002213"
     CDS             complement(342..1229)
                     /gene="rarD"
                     /locus_tag="ACHKAU_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO5531275.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1281..1748)
                     /gene="yigI"
                     /locus_tag="ACHKAU_002214"
     CDS             complement(1281..1748)
                     /gene="yigI"
                     /locus_tag="ACHKAU_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO5531276.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1913..2782
                     /gene="pldA"
                     /locus_tag="ACHKAU_002215"
     CDS             1913..2782
                     /gene="pldA"
                     /locus_tag="ACHKAU_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO5531277.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2915..4744
                     /gene="recQ"
                     /locus_tag="ACHKAU_002216"
     CDS             2915..4744
                     /gene="recQ"
                     /locus_tag="ACHKAU_002216"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO5531278.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4808..5428
                     /gene="rhtC"
                     /locus_tag="ACHKAU_002217"
     CDS             4808..5428
                     /gene="rhtC"
                     /locus_tag="ACHKAU_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO5531279.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5490..6110)
                     /gene="rhtB"
                     /locus_tag="ACHKAU_002218"
     CDS             complement(5490..6110)
                     /gene="rhtB"
                     /locus_tag="ACHKAU_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO5531280.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6221..7243
                     /gene="pldB"
                     /locus_tag="ACHKAU_002219"
     CDS             6221..7243
                     /gene="pldB"
                     /locus_tag="ACHKAU_002219"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO5531281.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7251..8051
                     /gene="yigL"
                     /locus_tag="ACHKAU_002220"
     CDS             7251..8051
                     /gene="yigL"
                     /locus_tag="ACHKAU_002220"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO5531282.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8127..9026
                     /gene="bioP"
                     /locus_tag="ACHKAU_002221"
     CDS             8127..9026
                     /gene="bioP"
                     /locus_tag="ACHKAU_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO5531283.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8914..9867)
                     /gene="metR"
                     /locus_tag="ACHKAU_002222"
     CDS             complement(8914..9867)
                     /gene="metR"
                     /locus_tag="ACHKAU_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO5531284.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9985..12246
                     /gene="metE"
                     /locus_tag="ACHKAU_002223"
     CDS             9985..12246
                     /gene="metE"
                     /locus_tag="ACHKAU_002223"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO5531285.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12285..13100)
                     /gene="ysgA"
                     /locus_tag="ACHKAU_002224"
     CDS             complement(12285..13100)
                     /gene="ysgA"
                     /locus_tag="ACHKAU_002224"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO5531286.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13362..14123
                     /gene="udp"
                     /locus_tag="ACHKAU_002225"
     CDS             13362..14123
                     /gene="udp"
                     /locus_tag="ACHKAU_002225"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO5531287.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14264..15691
                     /gene="rmuC"
                     /locus_tag="ACHKAU_002226"
     CDS             14264..15691
                     /gene="rmuC"
                     /locus_tag="ACHKAU_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO5531288.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15786..16541
                     /gene="ubiE"
                     /locus_tag="ACHKAU_002227"
     CDS             15786..16541
                     /gene="ubiE"
                     /locus_tag="ACHKAU_002227"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO5531289.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16555..17160
                     /gene="ubiJ"
                     /locus_tag="ACHKAU_002228"
     CDS             16555..17160
                     /gene="ubiJ"
                     /locus_tag="ACHKAU_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO5531290.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17157..18797
                     /gene="ubiB"
                     /locus_tag="ACHKAU_002229"
     CDS             17157..18797
                     /gene="ubiB"
                     /locus_tag="ACHKAU_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO5531291.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18876..19145
                     /gene="tatA"
                     /locus_tag="ACHKAU_002230"
     CDS             18876..19145
                     /gene="tatA"
                     /locus_tag="ACHKAU_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO5531292.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19149..19664
                     /gene="tatB"
                     /locus_tag="ACHKAU_002231"
     CDS             19149..19664
                     /gene="tatB"
                     /locus_tag="ACHKAU_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO5531293.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19667..20443
                     /gene="tatC"
                     /locus_tag="ACHKAU_002232"
     CDS             19667..20443
                     /gene="tatC"
                     /locus_tag="ACHKAU_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO5531294.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20485..21267
                     /gene="tatD"
                     /locus_tag="ACHKAU_002233"
     CDS             20485..21267
                     /gene="tatD"
                     /locus_tag="ACHKAU_002233"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO5531295.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21264..21752)
                     /gene="rfaH"
                     /locus_tag="ACHKAU_002234"
     CDS             complement(21264..21752)
                     /gene="rfaH"
                     /locus_tag="ACHKAU_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO5531296.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21919..23412
                     /gene="ubiD"
                     /locus_tag="ACHKAU_002235"
     CDS             21919..23412
                     /gene="ubiD"
                     /locus_tag="ACHKAU_002235"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO5531297.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23458..24159
                     /gene="fre"
                     /locus_tag="ACHKAU_002236"
     CDS             23458..24159
                     /gene="fre"
                     /locus_tag="ACHKAU_002236"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO5531298.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24351..25514)
                     /gene="fadA"
                     /locus_tag="ACHKAU_002237"
     CDS             complement(24351..25514)
                     /gene="fadA"
                     /locus_tag="ACHKAU_002237"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO5531299.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25524..27713)
                     /gene="fadB"
                     /locus_tag="ACHKAU_002238"
     CDS             complement(25524..27713)
                     /gene="fadB"
                     /locus_tag="ACHKAU_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO5531300.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27903..29234
                     /gene="pepQ"
                     /locus_tag="ACHKAU_002239"
     CDS             27903..29234
                     /gene="pepQ"
                     /locus_tag="ACHKAU_002239"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO5531301.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29234..29848
                     /gene="yigZ"
                     /locus_tag="ACHKAU_002240"
     CDS             29234..29848
                     /gene="yigZ"
                     /locus_tag="ACHKAU_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO5531302.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29887..31338
                     /gene="trkH"
                     /locus_tag="ACHKAU_002241"
     CDS             29887..31338
                     /gene="trkH"
                     /locus_tag="ACHKAU_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO5531303.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31350..31895
                     /gene="hemG"
                     /locus_tag="ACHKAU_002242"
     CDS             31350..31895
                     /gene="hemG"
                     /locus_tag="ACHKAU_002242"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO5531304.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7453 a         8171 c         8795 g         7706 t
ORIGIN      
        1 aaatggtaac ttttggcagt tgttcttggg taaagaacct tacagcaaaa aatctgattt
       61 tgttaaggca tggttattgt ggtgggctat taccatcctt gttctattta ttttaagcct
      121 gactacgaca ttaaatttaa gaagtatagg tatagggtta agttttgggt tttgtcaaat
      181 ccttggtaga tatacgatgt ctctaggatg gagtaagtac tggggagttt tgggggtatt
      241 tcctgtaaca aatattgttc tttttcttat cttaattttt ttcaaaaaaa gataaataag
      301 tcagtgggta ggctctttaa acggcctacc catgttaatt cttaactttt tctacgctgc
      361 gtataaatcg catccatcac aaaaattgcc agcgccaccc aaataaaggc gaaagtcacc
      421 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
      481 gggccaatgt actggaaaaa gcctaacgtt gagagacgca agcgcgtggc agcggcggta
      541 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
      601 atcgggtttt gccccatatg gctggttgag ctgtcggcaa tagcaaacag gtaaattgcc
      661 gccacgggca gcagccacat ggtttcgatt aacatgccgg tttgcgcttc aacggcaatc
      721 ttcttgcgta ccagaccgta gaaggcaaaa ctaaatgcca gtcccagcgc gataataggt
      781 agcgaaccaa aagtccacag ctggactaac acgccacata tcgccagaat caccgccagc
      841 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
      901 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
      961 aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt
     1021 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
     1081 ataaagaaaa acgaccagat cacgcgatgc gtcaggattt catcggcagg cacgtagtaa
     1141 atcaacttga agtacgctgg cgctataccc caaataaaat aagcggcaag agcgagtaat
     1201 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
     1261 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
     1321 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
     1381 aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga
     1441 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     1501 cgggttaagg tacttcccac gcacaccaga cctgcggcga catccagcgc tgacgcaata
     1561 accccgccgt gcaaaatgct ttgcgcccag ttacccacca tcattggctg atttttaaag
     1621 gccagctgtg cgaactcttt ttcgtaacgc tccagctcca tccctaacgc gcggttaaat
     1681 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     1741 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt
     1801 tgttggtttc tactttagga agggataact aacggctatg gagttaagta tgtaaaatag
     1861 cccgcagaaa aatattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     1921 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc
     1981 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     2041 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatttacac
     2101 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     2161 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     2221 cccgaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     2281 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     2341 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     2401 ctctaacggg cgttccgacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     2461 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     2521 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     2581 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     2641 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     2701 cggctatggc gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtgggggt
     2761 tatgctaaac gatttgtttt gatgaacggt tgagtggttg gcaaatctgg aacccagcat
     2821 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     2881 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     2941 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     3001 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggtcg tcatgcccac
     3061 cggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     3121 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactgc aagccaacgg
     3181 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     3241 aggctgccgc accgggcaaa ttcgtctgct ttatatcgcc ccggaacgcc tgatgctgga
     3301 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgttg atgaagcgca
     3361 ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     3421 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     3481 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     3541 ccgtccgaat attcgctaca tgctgatgga gaagttcaaa ccgctcgatc agttgatgcg
     3601 ctacgtgcag gaacagcgcg gtaagtccgg cattatctac tgcaacagcc gggcgaaagt
     3661 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     3721 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg acctgcaaat
     3781 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     3841 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     3901 tgatggcctg cccgcggaag cgatgctgtt ttacgatccg gccgatatgg cgtggctgcg
     3961 ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgaacgtc ataaactcaa
     4021 tgcgatgggc gcttttgccg aagcgcaaac ttgccgtcgt ctggtactgc tgaactattt
     4081 tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     4141 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     4201 ttttggtatg ggctatgtgg tggaagtgat tcgcggcgct aataaccagc gtatccgcga
     4261 ctatggtcat gacaaactaa aagtctacgg tatgggccgt gataaaagtc atgaacattg
     4321 ggtgagcgtg atccgccagc tgattcacct cggtctggtg acgcaaaata ttgcccagca
     4381 ttctgcccta caactgacag aggctgcgcg tccggtgctg cgcggcgaat cctctttgca
     4441 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     4501 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga tagccgatga
     4561 aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca
     4621 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     4681 acgctttggc aaaccgttta tggcgctgat tcgtgcgcat gttgatggcg atgacgaaga
     4741 gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc ccccgcgagt
     4801 caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg cgcttatgag
     4861 ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc
     4921 gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga ttgcgctgct
     4981 tggcctgcat ttgattatcg aaaaaatggc ctggctgcat acgctgatta tggtgggcgg
     5041 tggcctgtat ctctgctgga tgggttacca gatgctacgc ggtgcactga aaaaagaggc
     5101 ggtttctgca cctgcgccac aggtcgagct ggcgaaaagt gggcgcagtt tcctgaaagg
     5161 tttactgacc aatctcgcta atccgaaagc gattatctac tttggctcgg tgttctcatt
     5221 gtttgtcggt gataacgttg gcactaccgc gcgctggggc atttttgcgc tgatcattgt
     5281 cgaaacgctg gcgtggttta ccgtcgttgc cagcctgttt gccctgccgc aaatgcgccg
     5341 tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt
     5401 tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa
     5461 ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct
     5521 cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct
     5581 ttaatccata gagcaatccg ttgagcaagg gtggcgtaac cgatcatcac aataatatcg
     5641 accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg
     5701 atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca
     5761 aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaaggtca
     5821 attgcaccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc
     5881 cacttcaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca
     5941 accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg
     6001 cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg
     6061 cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc
     6121 cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgcta
     6181 aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga
     6241 ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg
     6301 gcgtcagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg
     6361 ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta
     6421 cgtaaaatat gcggaactgg cctatgacct gttccatttg gggtttgatg tcttaatcat
     6481 cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt
     6541 taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcagcagg aggttcagcc
     6601 cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt
     6661 atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg
     6721 gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca
     6781 tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc
     6841 tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacttacgct tctatgctga
     6901 tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc
     6961 tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc tcttacaagc
     7021 tgaagaggaa cgcgtggtgg ataaccgcat gcatgaccgt ttttgtgaac tccgcaccgc
     7081 cgcgggccat cctgtcgaag gaggacggcc gttggtaatt aaaggtgctt accatgagat
     7141 cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg attttttcaa
     7201 caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg
     7261 ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg
     7321 ccaaagaaac tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc
     7381 gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gattaagtct tacatgatta
     7441 cctccaatgg tgcgcgcgtt cacgatctgg atggtaatct gatttttgct cataacctgg
     7501 atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatccggac atcattacta
     7561 acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag atgcgctttt
     7621 ttaaagaagc ggtgttcaaa tatgcgctgt atgagcctgg attactggag ccggaaggcg
     7681 tcagcaaagt gtttttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga
     7741 ttaacgctcg ctggggcgat cgcgtcaacg tcagtttctc taccttaacc tgtctggaag
     7801 tgatggcggg cggcgtgtcg aaaggccatg cgctggaagc agtggcgaag aaactgggct
     7861 acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga
     7921 tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagac cttcatcccg
     7981 agctggaagt gattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct
     8041 atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat
     8101 actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg
     8161 ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg
     8221 tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt acccgtggca
     8281 atagcctgaa aacggtcggc ctgtatatgt tggtgggcgc gatgcagctc ggcgtgatgt
     8341 atatgctgag tttccgcgcg tatctctacc tgacggtctc cgaactgctg ctgttcaccg
     8401 tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct
     8461 ggggctatgc ctttagcgcc ttgttggcgg tgattggtgc cgggattatt cgctatgatc
     8521 aggtcaccga ccatttctgg actggcttgc tgctggtgca actctccaat atcacttttg
     8581 ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgcca cagcataacg
     8641 cctttgcgtg gttctatctt ggcgcgtttc tggtggcggt aattgcatgg ttcttgctgg
     8701 gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg
     8761 tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg
     8821 gaacgctggg cattatgaat aatatgcacg ttccggcagg gctgctggta aacctggcta
     8881 tctggcacca acagccgcac tggccaacgt tcattacagg cgcgctggtg atcctggcct
     8941 cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca
     9001 ggcgtgattg cgcgctgagc gaataaatgc ttctgtaatc ggctgacgct gctcgccatc
     9061 gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag
     9121 accctggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc
     9181 ggcaaccatc tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc
     9241 tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaatcaa
     9301 cagcgtctcg ctggcgagat cttccggtgt aattcgcgtt ttcgccgcca gtggatggtc
     9361 aggtgctaac accagacgca cttcatagtc gaacatcggc gaataatgca ggccactgcg
     9421 cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg
     9481 gtcaaacgtc acgcccgatt taaaatccat ctctacctgc ggccagttct tatggaaatt
     9541 ttctaacgcg ggcgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg
     9601 cgtctgctgc ggttcattgc aggcttgcag tgcctggcta atttgcggca gcacctggtt
     9661 tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac
     9721 aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg
     9781 cgtctgatgc aacgtcgccg cagcggctgc gagagagccg cagttccgca acgcttgtag
     9841 cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg
     9901 cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaatac
     9961 taaaaattcc ataaggggca tataatgaca attcttaatc acaccctcgg tttccctcgc
    10021 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    10081 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    10141 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgttctgacc
    10201 accagtctgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    10261 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    10321 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga attcgttaaa
    10381 ggccaacagt tcaaactgac ctggacgcag ctgctggagg aagtggacga ggcgctggcg
    10441 ctgggccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    10501 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    10561 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    10621 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    10681 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    10741 acgattactg cgctgcctgt tcagggtctg catgttgacc tcgtacatgg taaagatgac
    10801 gttgctgaac tgcacaagcg cctgccttct gactggttgc tgtctgcggg tctgatcaat
    10861 ggtcgtaacg tctggcgcgc cgatcttacc gagaaatatg cgcaaattaa ggacattgtc
    10921 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    10981 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    11041 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    11101 gagtggagcg ccccgattca ggcacgtcgt cactctaccc gcgtacataa tccggcggta
    11161 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    11221 cgtgccgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    11281 tctttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    11341 gacgccaata actaccgcac gggcattgcg gaacatatca ggcaggccat tgttgaacag
    11401 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    11461 tactttggcg agcacctcga cgggtttgtc tttacgcaaa acggttgggt acagagctac
    11521 ggttcccgct gcgtgaagcc accgattgtt attggtgacg ttagtcgccc ggcaccgatt
    11581 accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg
    11641 acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    11701 atcgccaaac agattgcgct ggcgctgcgt gatgaggtgg ccgatctgga agcggcaggg
    11761 attggtatca tccagattga cgaaccggcg ctgcgcgaag gtttaccact gcgtcgtagc
    11821 gactgggacg cctatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    11881 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    11941 tccattgcgg cgctggatgc agacgtcatc accatcgaaa cctcgcgttc cgacatggag
    12001 ttgctggagt cgtttgaaga gtttgattat ccgaatgaaa tcggtccagg cgtctatgac
    12061 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccctgctgaa gaaagcggca
    12121 aaacgcattc cggcagagcg cctgtgggtc aacccagact gtggcctgaa aacgcgcggc
    12181 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtctg
    12241 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    12301 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    12361 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta gacgataatc
    12421 tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga
    12481 atgctgttat cctgaccgcc atataagccg agaaccggcg cgttaagatc ggttgcgata
    12541 tcaacaggtt gtttcggtga attcagcgac ttatcgcccg tcagtttgcc gtaccacgcc
    12601 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    12661 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    12721 acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc
    12781 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    12841 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    12901 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    12961 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    13021 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    13081 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    13141 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    13201 gcagaatgaa gagtgattta tgtgatttgc atcacttttg gtgggtaaat ttatgcaacg
    13261 catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    13321 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    13381 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    13441 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    13501 accgcgaatt caccacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta
    13561 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    13621 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    13681 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    13741 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcca
    13801 ttggcgcgac aactcacgtt ggcgtgacag cttcttctga taccttctac ccaggtcagg
    13861 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    13921 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aaccctgctg accatgtgtg
    13981 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    14041 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    14101 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    14161 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    14221 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    14281 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    14341 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    14401 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgcg agttactcaa
    14461 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    14521 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    14581 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    14641 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    14701 tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    14761 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    14821 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    14881 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    14941 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    15001 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    15061 ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    15121 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    15181 aactctggat tacgtgctga tgtttattcc cgttgaaccc gctttttcac tggcgcttga
    15241 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    15301 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    15361 caacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    15421 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    15481 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    15541 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    15601 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    15661 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    15721 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    15781 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    15841 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    15901 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    15961 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    16021 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    16081 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    16141 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    16201 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    16261 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    16321 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    16381 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    16441 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    16501 cggcgggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    16561 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    16621 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    16681 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa
    16741 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    16801 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    16861 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    16921 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    16981 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    17041 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    17101 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    17161 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    17221 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    17281 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    17341 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    17401 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    17461 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt
    17521 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    17581 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    17641 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    17701 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    17761 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    17821 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    17881 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    17941 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    18001 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    18061 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    18121 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    18181 agctacacgt cgattcaggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    18241 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    18301 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    18361 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    18421 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    18481 gtattcctgc gctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    18541 cagaactgcc tgaattggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    18601 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    18661 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    18721 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    18781 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    18841 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    18901 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    18961 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    19021 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    19081 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    19141 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    19201 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    19261 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    19321 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    19381 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    19441 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    19501 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    19561 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    19621 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    19681 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    19741 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgatatct atcacctggt
    19801 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    19861 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    19921 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    19981 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggta tggcattcgc
    20041 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    20101 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    20161 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    20221 gccagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    20281 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    20341 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    20401 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    20461 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    20521 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    20581 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    20641 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    20701 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    20761 ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    20821 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    20881 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    20941 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    21001 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    21061 attccggcgg agaaattgct gatcgaaact gatgcgccgt atctgctccc gcgcgatctc
    21121 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    21181 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgctaatgtc
    21241 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    21301 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    21361 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    21421 taaggggttg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaatcacc
    21481 gccgatggga ctatcgctgg cgacgcgcca aagcgcacga agtggctgac accgcgggtc
    21541 gcgttgatag tcgtggtatg aatcacttct ggatcaaatt cgacaaacag gtagttggga
    21601 aacaatggct cactgactgc agtacgtttt ccacgcacga ttttttccag ggtgatcatc
    21661 ggtgccaggc aattcacagc ctgtctttcg aggtgttcct gggcacgttg aagttgcccg
    21721 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    21781 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    21841 agagttcacg ctaatttaac aaatttacag catcgcaaag atgaacgccg tataatgggc
    21901 gtagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcttgacg
    21961 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    22021 atcactgaaa ttgctgaccg cactttgcgt gccggtgggc ctgcgctgtt gttcgaaaac
    22081 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    22141 atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    22201 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    22261 aagcaagtat tgaacatgcc gacaaagcgg ctgcgtggtg cgccctgcca acaaaaaatc
    22321 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    22381 gccgcgccgc tgattacctg ggggctgaca gtgacgcgcg gcccgcataa agagcggcag
    22441 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    22501 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    22561 ttcccggttt ctgtggcgct gggtgccgat cccgccacga ttctcggtgc agtcaccccc
    22621 gttccggata cactttcaga gtatgcgttt gccggattgc tgcgcggtac caagaccgaa
    22681 gtggtgaagt gtatctccaa tgaccttgaa gtgcccgcca gtgcggagat tgtgctggaa
    22741 gggtatatcg aacaaggtga aacggcgccg gaagggccgt atggcgacca caccggttac
    22801 tataacgaag tcgatagttt cccggtattt accgtgacgc atattaccca gcgtgaagat
    22861 gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg
    22921 gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt
    22981 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    23041 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    23101 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    23161 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    23221 attgattatc tggattttgc ctcgcctgtc tccgggctgg gttcaaaaat ggggctggat
    23281 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    23341 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    23401 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    23461 acaaccttaa gctgtaaagt gacctcggta gaagctatca cggataccgt atatcgtgtc
    23521 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    23581 gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa agggtttatc
    23641 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    23701 ctcaaagatc atcaaatcgt ggtcgatatt ccccacggag aagcgtggct gcgcgatgat
    23761 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    23821 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    23881 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa gcaccctggt
    23941 ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc
    24001 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    24061 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg
    24121 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    24181 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    24241 ccggcaatcg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    24301 tgcaggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    24361 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    24421 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacagccc
    24481 agcggatgac ccagcgcgat cgcaccaccg ttgaggttga tcttctcgtc aatctgctca
    24541 atcagtccca gatctttaat acatggcagg atctgcgcgg caaaggcttc gttcatctca
    24601 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    24661 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    24721 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    24781 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    24841 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    24901 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    24961 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    25021 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgctgttaa gcccatcatg
    25081 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    25141 ggcacatggc ccatatgctc cacgccgcca accagacatg cctgcgcatc gccggtcata
    25201 atcatccgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    25261 accgccggga cagagtgtgg tacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    25321 ccctgctcca gcgtctgctg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    25381 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttctgcacgc
    25441 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    25501 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    25561 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    25621 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    25681 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    25741 agcgccatat ccgcttccgc cggagtggcg ataatgcctt cctccagaca gcgcaccact
    25801 tcgttgacca tcgggatcat catgcgggcg ataatctcct cttcgctgaa atcgcgcttc
    25861 ggctggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    25921 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    25981 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatcctctg cgggaagcct
    26041 gctgccatga cagcctgggc atgatgcgcg gtgtcaatgc ccacgacatc cagcagatat
    26101 gccggaccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    26161 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    26221 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    26281 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    26341 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg tttcagcgcg
    26401 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    26461 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    26521 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gattgtggag
    26581 atcacgccag ccagtttcag accatcgatc ttgccgcgct caagctgctt gttcagcagt
    26641 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    26701 ggcacgcctt tccacgcaga ctggtaagcg atgccgccgc ccataatgcc tgcacccagc
    26761 accgcggcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    26821 tattgatcgt taaggaaaat gccgaccagt gcgcgggctt cgttggtatg cgccagcggg
    26881 acaaaacttt tgttttccag gtttaaggct tcttcacgac caaaacgggc cgcagcttca
    26941 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgctgtttg tgcgaccatc
    27001 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag tggttccagc
    27061 ttcggctgac gttttgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaaccgcc
    27121 tttgcgcctt caaccagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    27181 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagcgcact gtctgcgccc
    27241 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    27301 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    27361 cagccaccgc ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    27421 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    27481 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    27541 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    27601 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    27661 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    27721 tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc gaagtgtgta ttttgtgtta
    27781 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    27841 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    27901 aaatggaatc actggcctca ctctataaaa atcatatagc taccttacag gaacggactc
    27961 gcgatgcgct ggcgcgcttc aagctggatg cgttacttat tcactccggc gaactgttca
    28021 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    28081 tgccggtaac tcaggtgcca aactgctggc tgctggtgga tggcgtgaac aagccgaaac
    28141 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctctttct
    28201 ggactgaaga cgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    28261 ctgctgcgcg cgggaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    28321 gtattgaggc cagcaatatc aacccgaaag gggtgatcga ctacctgcat tactaccgct
    28381 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaacg
    28441 gtcatcgcgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaacattg
    28501 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    28561 tcaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    28621 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgccgct gacctgaccc
    28681 gtacctggtc ggcaaaaagt gacaacgatt acgcacagct ggtgaaagac gtaaatgatg
    28741 aacaactggc gctgatcgcg accatgaaag ctggcgttag ctatgtggat taccacatcc
    28801 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    28861 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt atcggccatc
    28921 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacgcacc
    28981 tcgcggcacc ggcaaaatat ccgtacctgc gctgcacccg tattctccag ccgggcatgg
    29041 tgttaaccat cgaaccgggt atctacttca ttgaatcgct actggcaccg tggcgtgaag
    29101 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    29161 ttcgtatcga agacaacgtg gtgatccacg aaaataacgt ggaaaacatg acccgggatc
    29221 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    29281 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    29341 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    29401 ggtggcgggt gcgccggatg attcacaaca gctgggtttc tctgacgacg gggagccggc
    29461 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgttg gggaaattac
    29521 cgctgtggtg gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    29581 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    29641 aaccgaatat actttgcaat gtgaatatca tcagttaacc ggcattgaag cgttgctggg
    29701 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    29761 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    29821 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    29881 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    29941 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    30001 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    30061 aaagagaaag gcgaacttaa atcccgtgag gggtttctga tagtggtgct gttctggacc
    30121 gtgctgggta gcgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    30181 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    30241 gggctggatt cgctccctca cgccatcctc ttttatcgcc agatgctgca atggtttggc
    30301 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    30361 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    30421 gcggaaacgg cgaaaaccct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    30481 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    30541 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgatag cccgactatt
    30601 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    30661 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    30721 ggcgtacagt ttacgctggt ggttatttgt accctcgtac tgtggtttca taatgtctac
    30781 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtgtcgat ggcgacaacc
    30841 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    30901 tgttcggctt ttatcggcgg ttgtgccggg tcaacgggcg gtggcctgaa agtgatccgc
    30961 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    31021 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    31081 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    31141 ggcgtggatg acttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    31201 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    31261 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tgctggtgct ctttaccccg
    31321 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    31381 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    31441 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaat tatgaccgtg
    31501 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    31561 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    31621 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    31681 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    31741 atcgctggta cgaccgtttt atgatcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    31801 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    31861 tcgcccattt aaccgacaaa ccgacgctga aataagcaca aagaataaaa aatgcgcggt
    31921 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    31981 actgacacgg aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaattccg
    32041 acagagaaag caaaaataaa tgcttgactc tgtagcggga aagcgtatta tgcacacccc
    32101 gcgccgctga gaaaaagcga agcgg
//
LOCUS       DAZFJF010000044        31618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFJF010000044 DAZFJF010000000
VERSION     DAZFJF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.044"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..425)
                     /locus_tag="ACHKAU_002243"
     CDS             complement(<1..425)
                     /locus_tag="ACHKAU_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFO5531305.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVAD"
     gene            610..756
                     /locus_tag="ACHKAU_002244"
     CDS             610..756
                     /locus_tag="ACHKAU_002244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531306.1"
                     /translation="MPELQKRGICDGMHRTGTLKKGRKESALEEITLVFDERFRFLLK
                     DKTI"
     gene            complement(849..2540)
                     /gene="fruA"
                     /locus_tag="ACHKAU_002245"
     CDS             complement(849..2540)
                     /gene="fruA"
                     /locus_tag="ACHKAU_002245"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO5531307.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2557..3495)
                     /gene="fruK"
                     /locus_tag="ACHKAU_002246"
     CDS             complement(2557..3495)
                     /gene="fruK"
                     /locus_tag="ACHKAU_002246"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO5531308.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3495..4625)
                     /gene="fruB"
                     /locus_tag="ACHKAU_002247"
     CDS             complement(3495..4625)
                     /gene="fruB"
                     /locus_tag="ACHKAU_002247"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO5531309.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIVVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4993..6174
                     /gene="setB"
                     /locus_tag="ACHKAU_002248"
     CDS             4993..6174
                     /gene="setB"
                     /locus_tag="ACHKAU_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO5531310.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAIIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(6171..6425)
                     /gene="yeiW"
                     /locus_tag="ACHKAU_002249"
     CDS             complement(6171..6425)
                     /gene="yeiW"
                     /locus_tag="ACHKAU_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5531311.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            6580..7152
                     /gene="yeiP"
                     /locus_tag="ACHKAU_002250"
     CDS             6580..7152
                     /gene="yeiP"
                     /locus_tag="ACHKAU_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO5531312.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            7375..8841
                     /gene="yeiQ"
                     /locus_tag="ACHKAU_002251"
     CDS             7375..8841
                     /gene="yeiQ"
                     /locus_tag="ACHKAU_002251"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5531313.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHMVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8959..9945
                     /gene="yeiR"
                     /locus_tag="ACHKAU_002252"
     CDS             8959..9945
                     /gene="yeiR"
                     /locus_tag="ACHKAU_002252"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO5531314.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9984..10697
                     /gene="lpxT"
                     /locus_tag="ACHKAU_002253"
     CDS             9984..10697
                     /gene="lpxT"
                     /locus_tag="ACHKAU_002253"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO5531315.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            11109..11675
                     /gene="mepS"
                     /locus_tag="ACHKAU_002254"
     CDS             11109..11675
                     /gene="mepS"
                     /locus_tag="ACHKAU_002254"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO5531316.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11856..13412
                     /gene="pdeN"
                     /locus_tag="ACHKAU_002255"
     CDS             11856..13412
                     /gene="pdeN"
                     /locus_tag="ACHKAU_002255"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5531317.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13494..15308
                     /gene="yejA"
                     /locus_tag="ACHKAU_002256"
     CDS             13494..15308
                     /gene="yejA"
                     /locus_tag="ACHKAU_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO5531318.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            15309..16403
                     /gene="yejB"
                     /locus_tag="ACHKAU_002257"
     CDS             15309..16403
                     /gene="yejB"
                     /locus_tag="ACHKAU_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO5531319.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16403..17428
                     /gene="yejE"
                     /locus_tag="ACHKAU_002258"
     CDS             16403..17428
                     /gene="yejE"
                     /locus_tag="ACHKAU_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5531320.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLSSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17430..19019
                     /gene="yejF"
                     /locus_tag="ACHKAU_002259"
     CDS             17430..19019
                     /gene="yejF"
                     /locus_tag="ACHKAU_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO5531321.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(19023..19367)
                     /gene="yejG"
                     /locus_tag="ACHKAU_002260"
     CDS             complement(19023..19367)
                     /gene="yejG"
                     /locus_tag="ACHKAU_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO5531322.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19700..20890)
                     /gene="bcr"
                     /locus_tag="ACHKAU_002261"
     CDS             complement(19700..20890)
                     /gene="bcr"
                     /locus_tag="ACHKAU_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO5531323.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(20918..21613)
                     /gene="rsuA"
                     /locus_tag="ACHKAU_002262"
     CDS             complement(20918..21613)
                     /gene="rsuA"
                     /locus_tag="ACHKAU_002262"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO5531324.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21762..23522
                     /gene="radD"
                     /locus_tag="ACHKAU_002263"
     CDS             21762..23522
                     /gene="radD"
                     /locus_tag="ACHKAU_002263"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5531325.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23647..23931
                     /gene="rplY"
                     /locus_tag="ACHKAU_002264"
     CDS             23647..23931
                     /gene="rplY"
                     /locus_tag="ACHKAU_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO5531326.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(24070..25077)
                     /gene="yejK"
                     /locus_tag="ACHKAU_002265"
     CDS             complement(24070..25077)
                     /gene="yejK"
                     /locus_tag="ACHKAU_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO5531327.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            25259..25486
                     /gene="yejL"
                     /locus_tag="ACHKAU_002266"
     CDS             25259..25486
                     /gene="yejL"
                     /locus_tag="ACHKAU_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO5531328.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25506..27266
                     /gene="yejM"
                     /locus_tag="ACHKAU_002267"
     CDS             25506..27266
                     /gene="yejM"
                     /locus_tag="ACHKAU_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO5531329.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            27341..27417
                     /locus_tag="ACHKAU_002268"
     tRNA            27341..27417
                     /locus_tag="ACHKAU_002268"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27375..27377,aa:Pro,seq:ggg)
     gene            27636..28877
                     /locus_tag="ACHKAU_002269"
     CDS             27636..28877
                     /locus_tag="ACHKAU_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO5531330.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28976..29206
                     /locus_tag="ACHKAU_002270"
     CDS             28976..29206
                     /locus_tag="ACHKAU_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531331.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            29374..29580
                     /locus_tag="ACHKAU_002271"
     CDS             29374..29580
                     /locus_tag="ACHKAU_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5531332.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
     gene            29595..30071
                     /locus_tag="ACHKAU_002272"
                     /pseudo
     CDS             29595..30071
                     /locus_tag="ACHKAU_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            30264..30602
                     /locus_tag="ACHKAU_002273"
                     /pseudo
     CDS             30264..30602
                     /locus_tag="ACHKAU_002273"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            30633..30824
                     /locus_tag="ACHKAU_002274"
     CDS             30633..30824
                     /locus_tag="ACHKAU_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5531333.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQEVCHVQ"
     gene            30814..31056
                     /locus_tag="ACHKAU_002275"
     CDS             30814..31056
                     /locus_tag="ACHKAU_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531334.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGLCVMIVLIP"
     gene            31029..31250
                     /locus_tag="ACHKAU_002276"
     CDS             31029..31250
                     /locus_tag="ACHKAU_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531335.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEAVLRKTTDGD"
     gene            31252..31485
                     /locus_tag="ACHKAU_002277"
     CDS             31252..31485
                     /locus_tag="ACHKAU_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531336.1"
                     /translation="MRDIYHETIDRAFSALAYAEGMYEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            31491..>31618
                     /locus_tag="ACHKAU_002278"
     CDS             31491..>31618
                     /locus_tag="ACHKAU_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5531337.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVT"
BASE COUNT         7777 a         8155 c         7850 g         7836 t
ORIGIN      
        1 cagtccgcga caatgacctt tgccctctga ataaattcac ggtgctgtgc gagatattca
       61 gctgtaatag cgttgctaat attcatgtca tttatagcaa ccagcatttc accggtatta
      121 tcgagtaatg ataaataact cgacgtattt tctcccggca caatcaggca tttatcgaca
      181 taaacgccag attgattggt ttgcgttagc agcgattgac cataaaaatc actacctact
      241 gcgctcagta accaggcttt gttacccagc aacgccaggt tttgtgcaat atttcgccct
      301 actccaccag gtgtaaattt tattttacct ggatttgaat ccgcataatt taatgattca
      361 tgtgaatatc cggcgacatc aatattcgcc gaacctataa ttacgacata atccttttcg
      421 cgcatagcac gtcctctggt aaaaaattac caaccctgtc aatccacaag aatatagata
      481 ctgcaaagac agtttaaaca tgtgttcata tttaaacatg tgctcatagt atgtatgttt
      541 tacgtaaagt aaattgccgc cagagaggca aagcgggcag atttgagcag gctcacaaaa
      601 tgcgattaaa tgccagaatt acaaaagcga ggaatttgtg acgggatgca cagaacagga
      661 acactaaaaa agggcagaaa agaatctgcc cttgaggaaa taaccttagt gtttgatgaa
      721 cgtttcaggt ttctactgaa agataaaacc atttaaattc ataaaattac attcacaaac
      781 cggtcattct ctttgcgctc atccaatgat acagggcagt aaatttctgc cctgtaacac
      841 accttttatt acgcggcttt cgctactgcg tccacttccg gacgtttcag gaaggcatag
      901 gccaaacccg ccaccagcgt accggcaata attgctacca ggtaacccag taccggcgta
      961 atagcgccag ggatcagcag aacaaacaga ccaccgtgcg gtgccatcag tttcgcacca
     1021 atcgccattg agattgcgcc agtcagcgcc ccaccaacga tacaacacgg cagcacacgc
     1081 atcggatcac gagcagcaaa cggaattgca ccttccgaaa tgaagcacag tcccaatacc
     1141 agagcggctt tgccaccttc ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt
     1201 gccagaccca ttgccagcgg tggcaccata cctgccgcca taatcgccgc catcgggcca
     1261 taggtttgag tactcagcag acccacaccg aatgcgtacg ctgctttgtt taccggaccg
     1321 cccatgtcag tacacatcat gccaccgagg atcgccccca gcagaaccgc attcgcagtc
     1381 cccatggtct gcagccagtg agtcaaccct tcgagaatgc cagcaactgg tttaccgatc
     1441 aggtagatca tcgccagacc gaccaccaga ctggaaatta gcggaatgat caggatcggt
     1501 ttcagcgcct ccatactctg tggcagtttc agttgcgtac tgattaactt cgcaatgtaa
     1561 ccagccagga agcccgcaat aataccgcca atgaagccag aaccggtgct gaccgccagc
     1621 ataccgccaa tcagacccgg cgtgagaccc ggacgatcgg caatggagaa ggcaatgtaa
     1681 cctgccagta ccggcaccat cagcgcaaag gctgaaccac caccaatctg catcagcgcc
     1741 gctgccaacg tacccggctc tttaaacgct tcgataccaa aagcaaaaga aagcgcgata
     1801 cacagaccac ctgcaacgac catcggcagc atgtaagaga cgcccgtcag caagtgacgg
     1861 tatgcgcctg cactctcttt cttaccttca gaggtcgccg tttgagcttt gcccgccggt
     1921 tcatacggcg ttgcttcagc aaccgcttta tccagttcct gcgcggtttt cttcagcgcc
     1981 agaccggtag aggtacgata catcggttta ccagcaaatt tcgccagatc cacttcgata
     2041 tctgccgcca caatcaccag atccgctgcg gctacttctt cgggggtgat tgcattaccc
     2101 gcaccaacag aaccacgggt ttcaactttc acccaccagc cacgtttttt cgcttcggtt
     2161 tcaatggctt cagccgccat aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca
     2221 actacgcgtt tcggaccgct ggcggcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa
     2281 ggtttcgcat ggcctttggc ttcactcagg aacagctcag ggtgcgcaac tgcccgggaa
     2341 atatcaccca gccagacatt tttaccgttc agcgcgctgt cattcgggat ggaatcaccg
     2401 agaacaatcg ccatttctgc gtcgttcgga ttgtcgatga tttccagttt tgcttttcgc
     2461 gccgccgcgc ccagcagggt cttcgccata taggcgcgtg cctgaccgag attagcgtca
     2521 ataatcagca gcgttttcat tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac
     2581 gcgcgccatc attgcggcca actgcggacg atcggtaata cccacattgc tttgacttac
     2641 cgccagggct gcaacagcag tcgccagacg cagtgtgtgt tcactggatt cacgcatcag
     2701 caagccataa atcaggccac caaccataga atcccctgcg ccaacggtgc ttacgacatc
     2761 gactgacggt ggtttggcga tccattcgcc ggaggcatta acccaaagcg cgccttcggc
     2821 acccagtgaa ataacaacat gcgcaatacc ttgttcacgt agcgcatgtg cagcttcaat
     2881 cacatctttc atttcaggca gtttacggcc tgcccagatt tccaactcgc ggcggttagg
     2941 tttcaccagc cacggtgccg ctttcaaacc tgctactaac gcttcacggc tactatcaaa
     3001 gataatgcaa ggacactgac tacgcaggcg agtcatccag tcggtgaacg cttccgggct
     3061 gacgcctgac ggtaagcttc cgctgacaca gaccatatcg aactgaccga gccagctcag
     3121 agaatcagtc acaaagcgtt cccagtctgc gggggtgact tcaaaacccg agaagttgaa
     3181 gtcggtcact tcgccgtctt tttccgtcag cttaacgtta attcgggtgc gcccctgtac
     3241 aacctggaaa cggttggcaa tgcccagctc gctgaacagt tgctgaaaac catcctgatt
     3301 gtctttaccc aggaagccgc caacggtgac atcaattccc aggtctttta atactttggc
     3361 cacgttgatg cctttacccg ccgcatgcag accggtggtt ttcaccaggt tcacttcgcc
     3421 gcgttcaatt tccgggcaga aaccaacaag gtcataagcc ggattaaggg tgatagtagc
     3481 aacacgtctg ctcattatgc gccctcccca agaccagcag cgatagcgtc gccgattgct
     3541 ttcagcgcct gttcagcatc tgcaccctgg gcggtaaagc gtaggcgatg acctttctta
     3601 acgccaagtg ccacaacttt catcagacta cgtccgtttg ccggtttgcc ggtgccatca
     3661 aggtttgtca cggtaatatc actgttaaat tgtttaatgg tgttgaccag catggtacct
     3721 ggacgagcat gcaggccgtg ttcattgcgc accacaaact ccgcgcttaa cacgtcgtcg
     3781 gtcggcgcat catcgctggt cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag
     3841 cggtcagctt tattgtcgag caacaaatca gcgagacgct taagaaccac aatgggctga
     3901 tcgtcattca tcgccacact caccagcatg gctgccgttt cgccgtccac atcaaaagca
     3961 tttgccgcac ggcttaccgc aatcgcgcta cgcagattgc cttcggcgct atcgctcagc
     4021 cagataccct gtccgaggtt cagcggttgt tcattgatgg ctttggtgac gaaagtggcg
     4081 tcaactgccc tcgcctcttt cagacgcgca gcgttcagcg cctgaagagt cagcagatcg
     4141 ctggcggcga tatccagcgt cagcatttcg ttgtcgagct tcagctgctc actctgcttt
     4201 tcgcccatca gcaatgcgcg aagttcttct gctgttgttg ctgacttcag ttgttcagca
     4261 acggaatcat cgctcagtac gtgggtcagc tggcgtagca ggcccagatg ttcatccgag
     4321 ctggcagcaa taccgatcgc tacgtacgct acctgaccgt caccccaggt gacgccttcc
     4381 gtgaactgaa atacctgaac gccggttttc agcacctgat cgcgggtgtc ggtagtgccg
     4441 tgtggaatag caataccatt gccgaggaac gttgaggttt gctgttcgcg cgccagcatg
     4501 ccattgacgt agccttctgc tacgttaccg gcctgcacca gcgccgcagc gagctggcga
     4561 atcgcctctt ctttgtctcc ggccttttcg cccggatgga tgtcctgtac ggataactgg
     4621 aacatagttc tcctctcttg ctgaattgaa acgattcagc ttctatgaga aaaaaagcgc
     4681 caacctggct tagggttaaa gacaagatcg cgctgaaacg tttcaagaaa gcataatact
     4741 tctgtttcag cacgcaagga aagccgtaaa gttatgagca aaagtttgat gtgctgcaca
     4801 attttttgtc atttttccta attgctgatg ggaaaatccg ttatcagcaa tttcatttca
     4861 gcagcgtcag cagccaatca gcaacagtga aatggcattt tgattattcg tggcgctttt
     4921 gatttcgctg tctgtttatt ttctgacaag cagcgtaaac tccgcgtctt cctcttccag
     4981 tgatcgacca gcatgcataa ctcccccgca gtctccagcg cgaaatcgtt tgacctgacc
     5041 tcgacggcgt ttttaatcgt tgcctttctc accggtattg cgggcgctct gcaaaccccg
     5101 acactcagta tttttcttac cgatgaagta catgcccgtc cggcgatggt gggattcttc
     5161 tttaccggca gcgctatcat tgggattctg gtaagtcagt ttctcgccgg gcgctctgat
     5221 aagcgcggcg atcgcaaatc gctgattgtc ttttgctgcc tgttaggcgt gctggcctgc
     5281 accctttttg cctggaatcg caactacttt gttttgctat tcgttggcgt ctttcttagc
     5341 agctttggct cgaccgctaa cccgcaaatg tttgcccttg cccgtgaaca tgccgacaaa
     5401 accggacgtg aggcggtgat gttcagctct tttttacgcg ctcaggtttc actggcatgg
     5461 gtcattggcc caccgctggc ttatgcgtta gcgatgggtt tcagctttac ggtaatgtat
     5521 ctgagcgcag cggtagcgtt tattgtttgc ggcgtgatgg tgtggctgtt tttaccgtcg
     5581 atgcaaaaag agcttccgct ggcgaccggc acggttgaag cgccgcgccg taaccgtcgc
     5641 gatacgctac tgctgtttgt catttgtaca ttaatgtggg gctcgaacag cctgtacatc
     5701 atcaacatgc cgctatttat tatcaacgaa ctgcatcttc ccgagaaact ggccggtgtg
     5761 atgatgggga ccgccgccgg gctggaaatc ccgacgatgt tgattgccgg atatttcgcc
     5821 aaacgtctgg gtaagcgttt cttaatgcgc gttgctgccg tgggtggcgt ctgtttttac
     5881 gcaggaatgc tgatggcgca ttcacctgtc attctgttgg gcttgcagct gctaaatgct
     5941 atttttattg gcattctggg cggcatcggg atgctctatt ttcaggatct gatgcccggt
     6001 caggcgggtt cagccaccac gctctatacc aacacttcgc gcgtgggctg gatcatcgca
     6061 ggatcagtgg cgggcatcgt cgccgagatc tggaattatc acgctgtgtt ctggtttgcg
     6121 atggtgatga ttatcgccac tctgttttgc ttactgcgga ttaaagatgt ttaaggcgcg
     6181 gtcagcattt ccagatcgat aagccaggtc atggcctgct gacgcgagtt accgcacatt
     6241 tcagcgctgg cttgtaagcc tgcacagact ttcggtcgca gtggcgaggt gaaaatttta
     6301 cagcgttgct gttcatcaag ctgaatgcag ggcgtattgg cgggcttgcc atctggcata
     6361 ccgggaatag ggctggaaat tgaaggggca gtgcaacacg ccccgcaacc cggacggcat
     6421 tccatacgtt gttctcttaa ggttaaccac cggattgatg agcgcgcaca gtaccacctt
     6481 ttttgcacca gcaaaagtgc gaataccact tgcccgaaag gcccgtcgcg agtactttgt
     6541 cgcgatattt ttgacatttt cgactacagg aatttttcga tgccaagagc gaacgaaatt
     6601 aaaaaaggta tggtactgaa ttacaacggc aaactgctgc tggttaagga tattgatatt
     6661 cagtcgccca ctgcccgcgg tgccgctacg ctgtacaaaa tgcgtttttc tgatgtccgt
     6721 accgggctga aagtagaaga gcgtttcaaa ggtgatgata tcgttgacac cgtgacgctg
     6781 acccgccgtt acgttgactt ctcctatgtc gatggcaacg aatatgtctt tatggataaa
     6841 gaagactata ccccgtatac cttcaccaaa gatcagattg aagaagagtt gctgtttatg
     6901 ccagagggcg gcatgccgga catgcaggtg ctgacctggg acggtcaact gctggcgctt
     6961 gagctgccgc agaccgttga tctggaaatc gttgaaaccg caccaggcat caaaggggca
     7021 tctgccagcg cccgtaacaa accggcgaca ttgagcactg gtctggtgat tcaggtacca
     7081 gaatacttaa gcccgggcga aaaaattcgt atccatatcg aagaacgccg ttatatgggg
     7141 cgtgctgact aacttcagcc gcatgcagaa aagggatagc tcaggctgtc ccttttttaa
     7201 tttattatac caatcttcta ttttgcgctt cctgaacacc ttacgccctc aattttcact
     7261 cgttgattga tcgccctcac acttcatcgc attaacaatc cagaccaatt tcaattgctg
     7321 tcatataact ttacactgtc attgttaatt tatcgttact aagacgtgac tcctatgaag
     7381 acaattgcct ccgttacgct cccgcatcat gtacacgctc cacgctacga tcgccagcag
     7441 ttgcaatcac gtatcgttca ttttggcttt ggcgcctttc accgcgctca tcaggcgtta
     7501 ctgaccgatc gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga aatcagcttg
     7561 ttcagcggtg atcaactgat gagccagctc cgcgcacaga accatttata taccgtgctg
     7621 gagaaaggtg cggacggcaa tcaggtgata attgtcggtg ccgttcacga atgccttaat
     7681 gcaaaactgg attccttagc ggcaattatt gagaaatttt gcgagccaca ggtggcaatt
     7741 gtttccctga cgattaccga aaaaggctat tgtattgacc cggccaccgg tgcactcgac
     7801 accagtaatc cgcggattat tcacgatcta caaacccctg aagaacctca ctccgcaccg
     7861 ggtattctcg tcgaagcact gaaacgccgc cgtgagcgcg gccttacacc gtttaccgtg
     7921 ctctcctgcg acaatattcc cgacaatggt catatggtga aaaacgcggt gctgggaatg
     7981 gcagaaaaac gttcgccaga actcgccggg tggataaaag agcacgtcag ttttccggga
     8041 accatggtcg accgcattgt tccggctgca accgacgaat cactggtgga aatcagccag
     8101 catctggggg tgaatgatcc ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtg
     8161 gaagataact tcgtcgctgg gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat
     8221 gatgtcctgc catgggaaga gatgaaactg cggatgctta atggcagcca ctcttttctc
     8281 gcttatctgg gttacctctc aggattcgcc catatcagtg attgcatgca ggatcgcgca
     8341 tttcgccatg ccgccagaac attaatgctg gatgagcaag cgccgacact gcaaattaaa
     8401 gatgtcgatt taacacaata tgcggataag ttaattgcac gttttgctaa tccggcgctg
     8461 aaacataaga cctggcaaat cgcgatggat ggcagccaga aattaccgca acgcatgctg
     8521 gcaggtattc gcatacatca ggggcgcgaa acggactggt cgttgctggc attaggcgtt
     8581 gcaggctgga tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc
     8641 gatccgctta gcgataaaat tcgcgaactt gttgcgggca gcagcagtga acaacgcgta
     8701 accgccctgc tttccctgcg tgaagttttc ggtgatgatc tgccagataa cccgcatttt
     8761 gtgcaggcca tcgaacaagc ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta
     8821 ttaaacaccc tcaaaattta acgatttctg cggttaaagc ggatgaagct caccttcgtc
     8881 cgctctcccc ttctcttttc tgcttttttt agccaggatt aacgctcagt taacttacca
     8941 gagtattgcg gagccgttgt gaccaggacc aacctcatca ccggttttct cggcagcggg
     9001 aaaaccacgt cgattcttca tctgttagcc cataaagatc ccaacgaaaa atgggcggta
     9061 ctggttaatg aatttgggga agtcggaatt gatggtgctt tgctcgccga tagcggcgca
     9121 ttgctgaaag agatccccgg cggctgcatg tgctgcgtta atggtttacc catgcaggta
     9181 gggttgaata ccttactgcg tcagggaaaa ccagaccgct tgttgataga gccgaccggg
     9241 ctgggccatc cgaaacagat cctcgatctg ttaaccgcac cagtctatga accgtggata
     9301 gatctgcgcg ccaccttgtg cattctcgat ccgcgcttgc tgctggacga aaaaagcgcc
     9361 agcaatgaaa acttccgtga ccagctggct gccgcagaca tcattgtcgc caataaatcc
     9421 gaccgtgcga cgcccgaaag tgagcaagcg ctacagcgtt ggtggcagca aaatggtggc
     9481 gatcgacaat taattcacag tgagcatggg aaagttgacg gtcatcttct ggatttgccg
     9541 cgtcgcaatt tagccgagtt gcccgccagc gccgcgcatt ctcatcagca tgtcgtgaaa
     9601 aaagggttag cagcgttaag cctgccagag catcaacgct ggcgtcgcag tctgaacagc
     9661 gggcaaggat atcaggcctg cggctggata ttcgacgctg atacggtatt cgacaccatt
     9721 ggcattctgg aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt
     9781 cccgaagggc tggtacgaat caaccgtcag ggcgatgacc tgcacattga aacgcaaaac
     9841 gttgcgccac cggacagccg tattgagctg atttccagca gcgaagctga ctggaatgct
     9901 ttgcagagcg cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt
     9961 tcagtaagat aattagagaa aatatgatta aaaatttgcc gcaaatagtg ttgttgaata
    10021 ttgtcggcct cgcgctgttt ctttcctggt atattcccgt taatcatgga ttctggttgc
    10081 cgattgatgc ggatattttt tatttcttta atcagaaact ggtcgaaagt aaggcctttt
    10141 tgtggctggt tgcattgacc aacaatcgcg ctttcgacgg ttgttctctg ctggcgatgg
    10201 gaatgttgat gctgagtttc tggctgaaag aaaacgcccc tggcagacga cgtatcgtga
    10261 ttatgggtct ggtcatgcta ttaactgcag tggtattaaa ccagctgggt caggcattaa
    10321 ttcctgtaaa acgggccagc ccaacattga cttttaccga tattaaccgc gtcagcgaac
    10381 tgctctctgt tcccacgaaa gatgcctcac gagatagctt tcccggcgat cacggcatga
    10441 tgctgcttat tttttcggca ttcatgtggc gttatttcgg caaagttgca ggccttatcg
    10501 cccttattat ttttgtggtt tttgcatttc ccagagtaat gattggcgca cactggttta
    10561 ctgacatcat tgtcggttcg atgaccgtga tattgatcgg tttgccctgg gtgttgctga
    10621 cgccattaag tgatcgatta atcacctttt ttgacaaatc actaccggga aaaaacaaac
    10681 atttccaaaa caaataactc acagtaatta acatcatcag gcttattttt atagtgagga
    10741 taatcctgat gatgcgcacc gtgctttcat ccatcgcgcg caaaaatcat tctctaagta
    10801 aatgaatgga ttgcatgcgt ttcactcaat cgtactttaa ttgaccaatc ccgcttatta
    10861 actttctgta tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc
    10921 aatcgggtaa tctcgaactc gttttgcctc ggcggtagat tatcctcaca gcatataatt
    10981 ttgtgcgtta gtccacagat ttggccttaa ggaattgttt caacatgccc aggtaattag
    11041 tctcgtgtcg cttggcattt ttttataacg atatttgtcg ttaaggactt caagggaaaa
    11101 caaacaacat ggtcaaatct caaccgattt tgagatatat cttgcgcggg atccccgcga
    11161 ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa caccgcaaag aatatgcatc
    11221 ctgagacacg tgcagtgggt agtgaaacat catcactgca agcttctcag gatgaatttg
    11281 aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat gctgactgga
    11341 aaggcgtacg ttatcgtctg ggcggcagca ctaaaaaagg tatcgattgt tctggttttg
    11401 tacaacgtac attccgtgag caatttggct tagaacttcc gcgttcgact tacgaacagc
    11461 aggaaatggg taaatctgtt tcccgcagta atttgcgtac gggtgattta gttctgttcc
    11521 gtgccggttc aacgggacgc catgtcggta tttatatcgg caacaatcag tttgtccatg
    11581 cttccaccag cagtggtgtt attatttcca gcatgaatga accgtactgg aagaagcgtt
    11641 acaacgaagc acgccgggtt ctcagccgca gctaataaac cgtttggatg caatcccttg
    11701 gctatcctga cgagttaact gaaagcactg cttaggcagt gcttttttgt tttcattcat
    11761 cagagaaaat gatgtttccg cgtcttgatc caggctatag tccggtcatt gttatctttt
    11821 aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt catacgcgct cccaattctg
    11881 gacgtaagct cctgcttacc tgcattgttg caggcgtgat gattgcgata ctggtgagtt
    11941 gccttcaatt tttagtggcc tggcataagc acgaagtcaa atacgacaca ctgattatcg
    12001 acgtacaaaa gtatctcgat acctattttg ccgacctgaa atccactact gaccggcttc
    12061 agccgctgac cttagatacc tgccagcagg ctaaccccga actgaccgct cgcgcggcgt
    12121 ttagcatgaa tgtccgtacg tttgtgctgg tgaaagataa aaaaacattc tgttcatctg
    12181 cgactggtga gatggacatt ccactcaatg aattgattcc ggcgctcgac attaataaaa
    12241 acgtcgatat ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca
    12301 tctggtatcg caaccctttg ctgaaaaata gcggtgtgtt tgccgctctg aatctcaacc
    12361 tgacgccttc tctcttttat agttcacggc aggaagatta cgatggcgtc gccctcatta
    12421 ttggcaatac tgcgctatct accttttctt cacgtttgat gaacgttaat gaattaaccg
    12481 acatgccggt ccgtgaaact aaaattgcgg gcattcctct gaccgttcgg ctttatgcgg
    12541 atgactggac atggaacgat gtgtggtacg catttttact gggcggcatg agtggaactg
    12601 tcgttggcct gctctgctat tacctgatga gcgtacgtat gcgccccggc agagaaatca
    12661 tgaccgccat caagcgcgaa caattttacg tggcgtatca accggtggtg gatacacaag
    12721 ctttgcgagt aacgggcctg gaagtactgc tacgctggcg gcatcctgtc gcgggagaaa
    12781 ttcccccgga tgccttcatt aactttgccg aatcgcaaaa gatgattgtg ccgctgactc
    12841 agcacctgtt tgagttaatt gcccgcgatg ccgcagaatt agaaaaagtg ctgccagtag
    12901 gcgtcaaatt tggtattaac attgcgccgg accatctgca cagcgaaagc tttaaagcag
    12961 atatccagaa actgctcact tccctacccg cacaccattt ccagattgtg ctggaaatta
    13021 ccgagcgcga tatgctgaaa gagcaagaag ccacacaact cttcgcctgg ctgcactcgg
    13081 tcggcgtaga aattgctatt gatgacttcg gcaccgggca cagcgcgctt atctatcttg
    13141 agcgttttac gctcgattat ctgaaaattg accgtggatt tatcaacgcc atcggtacgg
    13201 aaacgatcac ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgt ctcaatatgc
    13261 tgacagttgc tgaaggggtc gaaacgccgg aacaagcgcg atggctaagc gaacgcggcg
    13321 ttaatttcat gcaaggctac tggattagtc gcccgttacc gctggacgat tttgtccgct
    13381 ggctgaagaa accgtatacg ccgcagtggt aaggtgtgct tacgtccctt attattcata
    13441 gtgaaagcat gccggattgc ggctaatgat gagtaaaagg aaatccgttg cagatgattg
    13501 tgcgcatact gctgctgttt atcgctctgt tcacctttgg tgtgcaggcg caggctatca
    13561 aggaaagcta tgcctttgcc gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt
    13621 ttgattatgt gaaccccgcc gcgccaaaag gtgggcagat aacgttgtca gccctcggca
    13681 ccttcgatac tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc accgagcagc
    13741 tgtacgacac gctatttacg acttccgatg acgaaccagg cagttattac ccgctgattg
    13801 ctgaaagcgc acgctatgct gacgattatt cctgggtgga ggtcgctatt aatccgcgcg
    13861 cccgttttca tgatggttcg cccattactg cccgcgatgt agagtttact tttcaaaaat
    13921 ttatgaccga aggcgtgccg caatttcgtc tggtctacaa aggcaccacc gtcaaagcca
    13981 ttgcgccgtt aaccgtgcgc attgagttag ctaaacccgg caaagaagat atgttgagtc
    14041 tgttttcgct gccggtattt ccagaaaagt actggaagga tcacaaactt agcgacccgc
    14101 tcgccacgcc tccgcttgcc agtggtccgt accgcattac gtcctggaaa atggggcaaa
    14161 atattgtcta ttcccgcgtg aaagattact gggcagcaaa cttaccggta aaccgcggac
    14221 gctggaattt cgacaccatt cgctacgatt attacctcga tgataatgtc gcctttgaag
    14281 cgtttaaagc aggtgccttt gatttgcgta tggaaaacga cgccaaaaac tgggccacgc
    14341 gttataccgg taaaaatttc gataaaaaat acatcatcaa agatgagcaa aagaacgaat
    14401 cagcccagga tacgcgctgg ctggcgttta atatccaacg tccagtattc agcgatcgcc
    14461 gggtgcggga agcaatcact ctcgcctttg actttgaatg gatgaacaag gcgttgtttt
    14521 acaatgcctg gagtcgcacg aacagttatt ttcagaatac cgaatacgcg gccagaaatt
    14581 accccgacgc cgcggagctg gtgcttctgg caccaatgaa aaaagatcta ccgccagaag
    14641 tcttcacaca aatctaccag ccgccggtat ccaaaggcga tggctacgat cgtgacaacc
    14701 tgttaaaagc cgacaaactt ctcaacgaag cgggctgggt gctgaagggt cagcaacgcg
    14761 ttaatgccac aacgggtcag ccactcagct ttgaattatt gcttcccgca agcagcaata
    14821 gtcagtgggt attgccgttc cagcacagcc tgcaacggct gggtatcaac atggacattc
    14881 gcaaggtgga taactcgcaa atcactaacc gcatgcgcag tcgcgactat gacatgatgc
    14941 cgcgcgtatg gcgggcgatg ccgtggccca gttccgattt acagatttcc tggtcatcgg
    15001 aatatatcaa ttccacttat aatgcccccg gcgtgcaaag cccggttatc gactcgctga
    15061 tcaaccaaat tattgccgcg cagggaaata aagaaaaatt actgccgttg gggcgagcac
    15121 tggatcgcgt attaacgtgg aattattaca tgctgccaat gtggtacatg gcggaagacc
    15181 gtctcgcctg gtgggataaa ttctcccagc cggccgtgcg ccccatctat agcctcggta
    15241 tcgatacctg gtggtatgac gtcaataaag cggccaaact gccgtccgcc agcaaacagg
    15301 gagagtagat gggcgcttac ctgattcgcc gtctgttgct ggtgatccca acattatggg
    15361 cgattatcac catcaacttt ttcatcgtgc aaattgcgcc tggcggtccg gtcgaccagg
    15421 ccatcgccgc cattgagttt ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg
    15481 ttcgtgccag ccatgcgcaa acgggtgtcg gcaatatcag cgacagtaag taccgtggcg
    15541 gacgcggatt agatccagaa gtgatcgctg agatcactca tcgctacggt tttgataagc
    15601 cgatccacga acgttacttc aaaatgctct gggactacat ccgctttgat tttggcgata
    15661 gcctgtttcg cagcgcctcg gtgctgacgc tgattaaaga cagtctgccg gtttccatca
    15721 ccctcggatt gtggagcacg ctgattatct atctggtgtc gattccgtta ggcattcgca
    15781 aagctgttta taatgggagc cgctttgacg tctggagtag cgcatttatc atcatcggct
    15841 acgccattcc ggcctttttg tttgccatcc tgctgattgt cttcttcgcg ggcggcagct
    15901 atttcgacct gttccctcta cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt
    15961 atcagaaaat caccgattat ctgtggcata tcacgctgcc ggtgctggcg acagtgattg
    16021 gtggctttgc ggcgctgacc atgctgacaa aaaactcatt ccttgatgaa gtgcgcaagc
    16081 aatacgtggt gaccgcgcgt gcgaaagggg taagtgaaaa aaatattctc tggaaacatg
    16141 tgttccgcaa cgccatgctg ctggtgattg ccggttttcc ggcgacgttt atcagcatgt
    16201 tttttaccgg ctcgctgctg attgaggtga tgttttcact caatggtctg ggcttactgg
    16261 gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg tatattttca
    16321 ccctgattgg cctgctgctg aatattgtca gtgatatcag ctatacgctg gtcgatccgc
    16381 gtatagattt tgagggacgt taatgtcgcg actcagcccc gtcaatcagg cccgttgggc
    16441 gcgttttcgc cataaccgtc gcggctactg gtcgttatgg attttcctcg ttttgtttgg
    16501 tttgagtttg tgttctgaac ttatcgccaa cgataaaccg ttgctggtac gttatgacgg
    16561 cagttggtat ttcccgttgt tgaaaaacta cagcgaaagc gattttggcg gcccgctggc
    16621 aagtcaggct gattatcagg acccgtggct gaaacaacgg ctggaaaata acggctgggt
    16681 actgtgggca ccgattcgct ttggtgctac cagtatcaac tttgctaccg ataagccctt
    16741 cccttctcca ccctcccggc aaaactggct gggaacggat gccaacggcg gcgatgtgct
    16801 ggcacgtatt ctctatggca cgcggatctc ggttctgttt ggcctgatgc tgactctcag
    16861 ttccagcgtg atgggcgtgc tggcgggggc gctacaaggc tattacggcg gtaaagtcga
    16921 tctctggggg caacgcttta ttgaagtatg gtcggggatg ccgacgctgt ttttgattat
    16981 tttactttcc agcgtcgtac agcctaactt ctggtggctg ctggcaatta ctgtcttgtt
    17041 tggctggatg agtctggtcg gcgtggtgcg ggcggagttt ttacgcaccc gtaatttcga
    17101 ctacattcgt gcggcgcagg cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat
    17161 gttgcctaat gccatggtcg cgaccctcac ctttttaccg tttattttat gtagttcgat
    17221 aaccaccctg acctcactcg atttcctcgg cttcggtctg ccgctcggtt caccgtcact
    17281 cggtgaactg ctgttacaag ggaaaaataa ccttcaggct ccgtggcttg ggatcaccgc
    17341 cttcttgtcg gtggcgatat tattgtcttt gctgatcttt attggtgaag ccgtccgcga
    17401 cgcatttgat cctaataagg cggtgtagca tgacgcaaac tctgttagcg attgaagatt
    17461 tgtcggtggg ttttcgccat cagcaaaccg tacgtacagt agtcaatgat gtttcactac
    17521 agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta
    17581 ccgcgctgtc aattttacgc ctgctccctt ccccgccggt tgaatatctc tccggcgata
    17641 ttcgttttcg tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg
    17701 gtaataagat cgccatgatt tttcaagagc cgatggtgtc gttaaatcca ttgcataccc
    17761 tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc
    17821 gaggcgaaat tcttaactgc cttgatcgcg ttggtatccg ccaggcggca aaacggctga
    17881 cagattatcc gcatcagctc tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc
    17941 tgttaacgcg accggaatta ttaattgccg atgaaccgac caccgcactg gacgtctctg
    18001 tccaggcgca gattttacag ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc
    18061 tgtttattac tcataacctc agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc
    18121 aaaacggtcg ctgtgtcgag caaaataacg ccgctacgct atttgcctca cccactcatc
    18181 cttacacaca aaagctactc aacagtgaac cgtcaggcga cccggtgccg ttgccagaac
    18241 ctgcctcaac gttgctggat gttgaacagc ttcaggttgc cttccccatt cgcaaaggga
    18301 ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa catcagtttt acgctacgag
    18361 cgggtgaaac actgggttta gtgggcgagt ccggttccgg gaaaagtacg acgggactgg
    18421 cgctgctgcg actgattaat tctcagggca gcatcatctt tgacggtcag ccactgcaaa
    18481 atttaaatcg ccgccagctg ttacctattc gtcatcgcat tcaggtggta tttcaggatc
    18541 caaactcctc gctcaaccca cgactcaacg ttttgcagat tattgaggaa ggcttacggg
    18601 ttcaccagcc gacgctttct gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg
    18661 aagtgggatt agatcctgaa acacgccacc gttatccggc ggagttctct ggtggtcagc
    18721 gacaacgtat tgcgattgcc agggcattaa ttcttaagcc ctcgctgatc atacttgatg
    18781 aaccgacatc atcactcgac aaaacggtac aggcgcaaat attgacgcta ttgaaatcat
    18841 tgcaacaaaa gcatcaactg gcctatttgt ttatcagcca cgatttgcac gttgtccgcg
    18901 cgttatgtca tcaggttatc gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg
    18961 cgcgcgtgtt tgccacaccg cagcaggagt atacgcgtca gctactggcg ttgagctgac
    19021 gcttaaaaag gattgtagtt tgaaaagggt tcggcaattg ccacaccaaa atttttcaat
    19081 cggcatgttg cagcaaactc gtcctggcga tttacaaaca ggcacggctc accttcacac
    19141 tccagcaccg aacatggaac ttcgatgtcg cttaacgcct ggcttagttt atacatcacc
    19201 gaccatgcat tatcaccatc cgggctaagc aatttaagag ccaccaggct gttgtcaccg
    19261 cacggtccat tttgcggaat cggttcaacc ttcgaacctg cgaaaccagc agaccagcga
    19321 tagttctgcg gcagtcgatg gacgattgag agttgtaatg aagtcactta ttttccccgg
    19381 aagcacattc acttcacaat ttgtttacat caattttaac acatcatcaa caatccgtct
    19441 ttatacagat cgtaaagatt gatgcctcgc ctgcgtggcc tctatggctc tgatttaagt
    19501 aatatctgcg ggcgtggtca cgtttgcggg gctatatgta cccgtttctg cgatctaact
    19561 caaccttttt aactacaatg atgtgacttt ttacataaat tgattttaca taaaataaac
    19621 atatatcggg ggaatgatag atttgtggtt gacatgcatc aacaaaggaa gccttttagc
    19681 ttcctcgttg tgcaatagat caccgttttt tcggtcgact ggcgtacaga cagaagagaa
    19741 tggagctgct tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg
    19801 caagagaaag caatgcgcca acaattgccc ctatgccaaa acggaaggtt cctgccagcg
    19861 aagatgccgt tcccgccata tggggaaact catcaagaat gaccgccatc gcattggatg
    19921 acaccatcga cacgcagccc acaaacgccg caacgccaac caccagcgac cagaatccca
    19981 gccccagcag cgcactgatg accatccacg ctgccataat aaattgtatc cacaaccccg
    20041 agcggaacat atttaacgcg ccaatgcggc gaacgaagcg gctgttaaaa atggtcatca
    20101 cgaacaggaa aacaatgttt agtgcaaagt aataaccaaa gttttccggc gcgacgtggt
    20161 taatttcaat gtagacaaac ggtccggcgc ttaagaacga gaacatcccg gcaaagctga
    20221 aaccactggc aagcatataa ctcaggacac gcttatgacg gaacagcgcc gcaaagttac
    20281 caatagtggt acgaatgtga aaaggctggc gacgctccgg tggtaaggtt tctttaatca
    20341 ggaagaaaat cattgccgaa gccagaatcg ccgctaatgc caggatccag aagatgtaat
    20401 gccagctcag ccacaccagc acccaaccgc cgacaatcgg tgccatcagc ggtgcaatag
    20461 tggtcaccag catgacaaac gacatcatcc gcgagaactc ttctttcggg taaatatcgc
    20521 gcatcagggc gttaatgacc acgctggccg cagccgccgc cagcccgtgg aagaaacgca
    20581 tcacaatcag ctgatcgatg gtgtttgcca acgcacacgc caccgcggcg gcggcaaaca
    20641 ccagcgtacc gccgagcacc accggcttac gcccgaagct gtctgccatc ggcccgtaga
    20701 ttaactgccc caacgcaaag cccagaatat aagtactgag ggtcatctgc gtactgcccg
    20761 caggtacgcc aaactgcgct gaaattaccg gtagcgcggg cagatacata tcaatcgaca
    20821 gcggcatcaa catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc
    20881 gggtggtcac aacgggctcc tgaaagtcat ggaaaagtta gacgacgctg gcaatttctt
    20941 cttcagttaa cggacgatat tcaccggggg ctaaatcagc atccagcgta ataccgccaa
    21001 tacgttcacg atgcagctca accacgtggt tacccacggc ggcgaacatg cgtttcacct
    21061 gatgataacg cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttccagca
    21121 ccgcaggctt agtgagatct ttttcgttat gcagctgcac gcctttagca aattgctctg
    21181 ccgtatcgtc agctacaggt gattccagtg tcaccagata ggtcttctcg caatgatggc
    21241 gcggagaagt aatgcggtgc gaccactgac catcatcagt catcagcacc agaccggtgg
    21301 tatcgatatc cagacgtcct gcggcgtgca gtttccaggc taccggttca tccagaaaat
    21361 agagcaccgt tgggtgatca gggtcgtccg tggagcaaac atagccctga ggcttattga
    21421 gcatgaagta acgtggacca tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat
    21481 gttcaggaag cagtttgaac gctgcattac gaacaatttc gccatcgacg gtgacacgat
    21541 tgccgcggat ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt
    21601 tatcaagtcg catgtgtgtg attttgcctg taaaaacgga ggtcgggcat tgacccgaaa
    21661 atctgaactg ttgtctgccc agtatagcgg tctaataaca tccctcaagg gaaaaagatc
    21721 catggcatac tattagcaga ataatctacc tacgcgagac catgattttt acactccgcc
    21781 catatcagca agaagccgtg gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg
    21841 ccgttatcgt gctgcccacc ggcgcaggta aaagcctggt gattgccgaa ctggcacggc
    21901 tggcgcgtgg tcgtgttctg gtgctggcac acgtcaaaga actggtggcg caaaaccatg
    21961 caaagtatca ggcgctgggg ctggaagccg atatttttgc cgctgggtta aagcgcaaag
    22021 agagccacgg taaagtggta tttggcagcg tgcagtcggt cgctcgtaat cttgatgcct
    22081 ttcagggtga attttcgctg ttgattgtcg atgaatgcca ccgtattggt gacgatgaag
    22141 agagccagta tcagcaaatc ctcactcacc tgactaaagt gaatccccac ttacgcctgc
    22201 tggggctgac tgccacgcct tttcgattgg gcaaaggctg gatctaccag ttccattatc
    22261 acggcatggt acgcggcgat gagaaagccc ttttccgtga ctgcatttat gagctgccgc
    22321 tgcgttatat gattaaacac ggctatctga cgccgccaga acgactggat atgccagtag
    22381 tgcaatacga tttcagccgc ttgcaggcac agagtaacgg gctgttcagc gaagctgatc
    22441 tcaaccgtga gctgaaaaaa cagcaacgca ttacgccaca tatcatcagc cagatcatgg
    22501 agttcgccga aaagcgcaaa ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag
    22561 agattgtggg attactgcct gccgaagatg ctgcactgat tactggcgac acccccggcg
    22621 ctgagcgcga tgtgttaatt gaagatttta aagcccagcg ttttcgctat ctggtcaacg
    22681 tcgcagtact gaccaccgga tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc
    22741 ctaccgaatc agtgagtctt taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg
    22801 gcaagactga ttgtttaatt cttgattatg cgggtaatcc tcacgatctc tacgcgccgg
    22861 aagttggtac accgaaaggc aaaagtgaca acgttccggt acaggttttc tgccctgcct
    22921 gcggttttgc caacaccttt tgggggaaaa cgaccgccga cgggacattg attgaacact
    22981 ttggtcgccg ctgtcaggga tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact
    23041 tccgtttccg ttttaaaaat tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc
    23101 gctgccgcga atgtgacacg gtactggttg atccggatga tatgttaaaa gcggcgctac
    23161 gactgaaaga tgcgctggta ttacgctgta gcggcatgtc tttgcagcat gggcacgacg
    23221 agaaaggcga atggttgaaa atcacctatt acgatgaaga cggcgcggat gtgagtgagc
    23281 gtttccgtct gcaaacgccc gcccagcgaa ctgccttcga gcagcttttt atccgcccgc
    23341 atacgcgcac accgggcatc cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc
    23401 aagccttact gcgacatccg gattttgtcg tcgcccgcat gaaaggtcag tactggcagg
    23461 tgcgtgaaaa agtgttcgat tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt
    23521 aatggtagtt ttgattgatg tataaggcga ttgagtatag aatctcgccc gcttttgcat
    23581 acgcaaagca gatcacttac ctgttgctgg gtcgcctgta gcaggattaa tttaagagag
    23641 aaagaaatgt ttactatcaa cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc
    23701 cgcctgcgtg ccgctaacaa gttcccggca atcatctacg gtggcaaaga agcaccgctg
    23761 gctatcgagc tggatcacga caaagtcatg aacatgcaag ctaaagctga attctacagc
    23821 gaagttctga ccatcgttgt tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag
    23881 cgtcacccgt acaaaccgaa gctgcagcac atcgacttcg ttcgcgctta attgctgaat
    23941 aagttgtaaa aaaccccgct ccggcggggt tttttgtatc tgcagattat gcctgattac
    24001 ggtattgcta ttttttgcag gccagataag gcgttcacgc cgcatctggc attcagaaca
    24061 caaaaccgat taattgccgc cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg
    24121 cgtgccttta atggtcaagg tatcggttgc tggatcccag aagatgcgtt cacctaacag
    24181 catcgcatca aagttaatag tcaagccacc gccgctaccg gcaaacttcg tcaactgacg
    24241 cagtgtgcta cggtctgccg ggaagctctc ttccagttcg tagccttttt cagccgcaaa
    24301 ctccgtgaag ctcacttcgc ttacgcctgc cagctctttc gacagcgatt ccagttcgat
    24361 ctcttcgcct gcctgcagct gctcattaca gtagctataa acctgctggc gcacgttctg
    24421 tcgctctgct ttatccagtt gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc
    24481 acggttttgc gctttcgcgt tcaatccttc gctggccccg aggaaatcca taaagaaatc
    24541 cgccactttg cgccctactc gccctttcag gaaagtgaga tagcgggtgg actctggatt
    24601 ggtttcccat tcggttaaat cgatacgcgc aacaatatcc gcatgattga tatcaagata
    24661 atgggttggg ttgatgtcga gattttcgtt aacgcgcata ctgctcaggt tgctcagaac
    24721 tgccaccagc agatactcca ccgccagata acggtagtgg caaaacagca cgaatccacc
    24781 gtcagcaaaa ggatatttcg ctaattcgtc acgcaggcgc ccggttgccg cgcggctaaa
    24841 tgccagaaag tcctcttcgc cctgacgctg taagcgcagc gtctgcgcca gttcactctc
    24901 ttcgctaaac aagccgtaag ctttattttt ggcgctatag acccgatgca gttctgccac
    24961 catctcaacg acggtttctg tcggttccag caatgaatcg cgcaacacca gctcaaggtt
    25021 ctgctcatca cgcttgataa gctggtgcag ggcaatctgg ttgatatcca gactcatgat
    25081 aaactctcct ttaagaccgg gcggtattca accaccgccg gttaagcgac gcaataaaag
    25141 atggcggtca ttttacagaa ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga
    25201 aaaaaaaccg ttgctacggt aatatgttgc cctttcatca actaactgat ttcgatttat
    25261 gccacaaatt tcccgctaca gtgatgaaca ggttgaacaa ttgctcgcag agctgctcaa
    25321 cgtactggaa aaacataagg ctccgaccga cctttccctg atggtgttag gcaacatggt
    25381 cactaacctt atcaacacca gcattgcccc ggcccaacgc caggcaattg ccaactcttt
    25441 tgcccgcgcc ttacagtcct ctatcaacga agacaaagcg cactaaggga aacagataac
    25501 tggttatggt aactcatcgt cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg
    25561 ggcactggtt tgcactgttc aatattctgc tttcgctcgt cattggcagc cgttacctgt
    25621 ttatcgccga ctggccgaca acgcttgctg gtcgcattta ttcctacgta agcattatcg
    25681 gccatttcag cttcctggtg ttcgccacct acttgctgat cctcttcccg ctgaccttta
    25741 tcgtcggctc ccagaggctg atgaggtttt tgtccgtcat tctggcaacg gcgggaatga
    25801 cgctattact gatcgatagc gaagtcttta ctcgtttcca tctccatctt aatcccatcg
    25861 tctggcaact ggttatcaac ccagacgaaa atgagatggc gcgcgactgg cagctgatgt
    25921 tcatcagcgt gccggttatt ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa
    25981 agctgcgcag cctgacgcgt cgtcgacgct tcgcgcgccc gctggccgca ttcttattta
    26041 tcgcctttat cgcctcgcat gtggtgtata tctgggccga tgccaacttc tatcgcccga
    26101 tcaccatgca gcgcgctaac ctgccgcttt cgtacccgat gacggcgcga cgttttcttg
    26161 agaagcatgg tctgcttgat gcgcaggagt atcaacgccg tcttattgag caaggtaatc
    26221 cagacgccgt ttccgtacag tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg
    26281 ggcagaatgt gctgttgatt actgtcgatg gcctgaacta ctcacgcttc gagaagcaga
    26341 tgcctgcgct ggcaggtttt gctgagcaaa atatttcgtt cacgcgccat atgagctccg
    26401 gcaacactac agacaacggc atctttggcc tgttctatgg catctcgccg agctatatgg
    26461 acggcattct gtcgacccgt acgcctgcgg cattaattac tgcgcttaat cagcaaggct
    26521 atcagctggg gttattctcg tcagatggct ttaccagccc gctgtatcgc caggcattgt
    26581 tgtcagattt ctcgatgccg agcgtacgca cccaatccga cgagcagacc gccacgcagt
    26641 ggatcaactg gctgggccgc tacgcacaag aagataaccg ctggttctcg tgggtctctt
    26701 tcaatggcac taacattgac gacagcaatc agcaggcatt tgcacggaaa tatagccggg
    26761 cggcaggcaa tgtcgatgac cagatcaacc gcgtgctcaa tgcactgcgt gattctggca
    26821 aactggacaa tacggttgtg attatcactg ccggtcgggg tattccgctg agcgaagacg
    26881 aagaaacctt tgactggtcc cacggtcatc tgcaggtgcc attagtgatt cactggccag
    26941 gcacgccggc gcagcgtatt aatgcgctga ctgatcatac cgatctgatg acgacgctga
    27001 tgcaacgcct gctacatgtc agcacacctg ccagcgaata ttcgcaaggt caggatttgt
    27061 tcaaccctca acgccgtcat tactgggtta ctgcagcgga taacgatacg ctggcaatta
    27121 ccaccccgaa aaagacgctg gtgctgaaca ataacggtaa ataccgcact tacaacttac
    27181 gtggtgaaag agtgaaagat gaaaaaccac agttaagttt gttattgcaa gtgctgacag
    27241 acgagaagcg ttttatcgct aactgattaa ttataaatca gttagcgaaa tatcttactt
    27301 gcaatcggtg tggaaaacgg tagtattagc agccacgagt cggcacgtag cgcagcctgg
    27361 tagcgcaccg tcatggggtg tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaat
    27421 cgcccaccgg atatcaccgg aaagcaccgg ataagacagg gataacccgc taatcacttg
    27481 attggcgggt ttttttatgt tttttagtca ccgaacatca ccggatttat aagctatcag
    27541 ccggattttt tagttatacg tttagttata cgggctggcg tataactaaa gccgtataac
    27601 taaaaccctg ttttttcgtg tggaatcata gacatatggc gagaaaaaca cacccattaa
    27661 ccacagtgca gatcaaagca gccagaccag cgcaaaagga gtacaccctg caggacggcg
    27721 gagggctttt tctcctggtc aaaccgtctg gatcaaaact ctggagattt tcctactacc
    27781 gaccatcgga caaaaaaaga atattgctga gttttggatc gcttgaagat gtttccctgg
    27841 ctgatgccag aaaacgccgt agcgagtaca ggacgttaat cagtgccgga actgacccgc
    27901 aggaccacga gaggcaaaaa agagagacag aggcccgaag acaagggaac acgttcgaaa
    27961 atgtggcggc ggcatggtac caggtgaaaa tcagccagaa tctggccccc aacacgatta
    28021 aagacatctg gcgttcgctg gataaatatg tattcccgtt catcggcaac acgccaatag
    28081 ataccctcac cgcccgaagg ttcgttgaag tgcttacccc catcaaggag cgcggcaacc
    28141 tggaaacact caagcgggtt ttacagcgcg ttaatgaggt aatggattac gccgccaaca
    28201 gtgggctgat tgatgccaat ccggctatga atgtgcgtaa ggcgtttccc tcacctgtaa
    28261 aaaaacatat gccaacaatc cgccccgaac agctgcccga gcttatgcag gctttatcag
    28321 tatcggcaac agaacggcag accagattac tgattgaatg gcagttactg accgtaaccc
    28381 gtcccgccga agcgtcatca acgcggtggg atgaaatcaa cctggacgcg aagcaatgga
    28441 cgatacctgc cggacgcatg aagatgcgca gggatcacgt tatcccgctt tccggtcagg
    28501 ctatggcggt gctggaggcc atgaaaccaa tcagccacca ccgcaattac gttttcccaa
    28561 gtctgaaaga cccacagcag ccgatgaaca gccagacagc taacgcagca ttgcggcgta
    28621 tgggattcgc tggcgtgctg gtgtctcatg gattacgcgc catattcagc acagcagcga
    28681 acgaggaagg attcgagccg gacgtaatag aggcggcact tgcccacgtc gacaccaacg
    28741 aagttagacg ggcatacaac cggagcaact acatagaaaa acgcatcgtg ctgatgcgct
    28801 ggtggggcga atttgtcgag gctgcggcga cgggcgtaac cctcgccagt ggtaaaaggg
    28861 gtatccgagc cgtgtagctg tacagaaaac cagtaaaaac tacgaaaacc atgtaaaacc
    28921 gtcgtataat tgcatcaaat ttaacgacaa ggccgtgaaa catgaaaccg ttaagatgca
    28981 aaaaaatatc agatgcaatt gcgacgggct gcaactggcc ctgatggttc agcatgaatt
    29041 ttggtcaacc tacgatccgg aggacagaac gacggcccca tcaaaaaaac aggtagtaga
    29101 ttttctggta tcccgtggcg cttccagaaa tctggcggta agtattgata aggtcgtacg
    29161 tccggcatct atgaagatcg gaggcaggcc caaaaaatgg cggtaacaat cctggaagcg
    29221 gcagaaatgc cgcttttttt ataattccat ttcaaatcat caatataaaa aacggtgtat
    29281 accgtttaaa aacggtggga actgttttta cccatatccg atgatttacc gtatttgtca
    29341 ccggaataca ccggattcac aaggtaaatc acgatggaag caatcagaaa aattatcttt
    29401 cgccaggaag taaaaaaaat tatccacatc aaggcagaca gcacgctgca aagcatgatc
    29461 aacgccggag aatttccgca gggttttcgc gttggtttac gccgtcgcgg atggtatgag
    29521 gatgatgtgt tgtcctggca gaaagaacgc gaagaggaag cacgcggaac ggctgcttaa
    29581 cgggtatcac agatatgaca aacacgaaaa aagcggcccc gatatggagc cgcctttctg
    29641 aacaattaac ccgctgcgcc ttatgtgtat gtgatcccaa acataagcac ggggatgata
    29701 gccgctatca ggctggtggg caatgcaatc agtctggttc agttcgttgc catacctgca
    29761 atgagcgctt ttccctgtac tctttaagga attgctcaag ggcaaaagca catggcgcga
    29821 atctttctga ttcatgctct atctttctgc gccgtctttt ccgtgccggt gataatgttt
    29881 tggtcaattc tttatcggtc attgtgttgt cctgcatagc aatgcgccgt aatacctcac
    29941 accacggcgc tgatagtttt tatcctttgg gttctatgcc gcgttgctgt aattctttgc
    30001 ggagaacgcg tttaatccaa gtggctaaag atgtgtcgcc atcggcctta gctgcttctt
    30061 ctagctgcgc tctgaagtct tccggtaatc gcatttggta tggaggcgaa cgtttttcta
    30121 atggtgttga catggtaatt acccttcatt actataccta catggtaatt accattgtac
    30181 ttgccattac acaaaaatca acgccccgca gtgctgggaa cacatacggg gcgtctaacc
    30241 aacaacgtaa actaggagcc gttatggttg ccgtaaatca tataccacac cttgtacaca
    30301 cacaaacggc ctttgtgtgg cgttttctgg ccctgaatat cggagaaaaa aaccagttga
    30361 tcgccagtgt cgaaaatagc tatgattccc gcgcacctca taaaacgggt gccgggattg
    30421 gcgtcctggg caaaactgaa gcgcataaca cgcgccccgc gtgttttttt gtgccgcata
    30481 gtcacacctt atcaatggtg ggctgtacgg gggcggagca atccgcgccg gtttcttcag
    30541 tgtccggtta cgccaaccct gtacagtccg ccaccagcga aattggcgtt tccggtggcg
    30601 gttatcttaa ccacactgag gaggctgcca acatggctac tatccctacc cttgtacatt
    30661 ctcaaaccgc ctttctctgg cgctttatca tctttggcgc gtcagaacat caaatcatcc
    30721 acgtaaccgc ctggacggaa cgcgaagcgc gtagccgttg cccgtccggt tgtgttgctg
    30781 tattcgccgc ccgtattcgt caggaggtgt gccatgtgca ataaaaccac accggacgca
    30841 gcagccgccg cgctcactac gctgatgcac gcgcttatcg atattgaatg cactgcagag
    30901 cttgcgcagg gagaagaaca gaaagaccgg acacagttcg ccctggaatg tatccgatac
    30961 atcgcaacgc ggtcgctgaa tgacgctaaa aatattcttg ttgctgattg tgaaaatggg
    31021 ggggggttat gcgtgatgat cgttttaatt ccctgaaaca agaattttcc ggcgttcctg
    31081 atgatgcggc tgatgcgctt tcgtcaatat ctgaaattat gcgggtggct tttttctttc
    31141 tttgcactga tgagcacaga gatacagggc taaatattct tgatattgcc gctaactatg
    31201 ctgatttcgt gacagaagct gttttaagaa aaacaacgga cggggattaa tatgcgtgat
    31261 atttaccacg aaacaataga ccgcgcattt agtgcccttg cttacgctga aggtatgtac
    31321 gaaatattgc gcatatggct tgaaacactt ggcgacaatg aacgcgacaa acaaaaatca
    31381 agaattgtca cggcattaat aacgcttctt gagcctgtaa taaatgagtt gcaggaaata
    31441 gagacattgc acgacagata taacgaacag cacaccggag aataaaaata atgaaactta
    31501 aatattctgg cttaactgcc agtggcaaca ctcaccctaa atttacgcgc ggtgatattt
    31561 accgcgacca gtacggcggc acggtaatga ttaagggcgt ggcgggacgg tgcgtaac
//
LOCUS       DAZFJF010000045        31498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFJF010000045 DAZFJF010000000
VERSION     DAZFJF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.045"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(167..430)
                     /gene="cedA"
                     /locus_tag="ACHKAU_002279"
     CDS             complement(167..430)
                     /gene="cedA"
                     /locus_tag="ACHKAU_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO5531338.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            613..2874
                     /gene="katE"
                     /locus_tag="ACHKAU_002280"
     CDS             613..2874
                     /gene="katE"
                     /locus_tag="ACHKAU_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO5531339.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVLVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3133..3891)
                     /gene="chbG"
                     /locus_tag="ACHKAU_002281"
     CDS             complement(3133..3891)
                     /gene="chbG"
                     /locus_tag="ACHKAU_002281"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO5531340.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3904..5256)
                     /gene="celF"
                     /locus_tag="ACHKAU_002282"
                     /gene_synonym="chbF"
     CDS             complement(3904..5256)
                     /gene="celF"
                     /locus_tag="ACHKAU_002282"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5531341.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5361..6203)
                     /gene="chbR"
                     /locus_tag="ACHKAU_002283"
     CDS             complement(5361..6203)
                     /gene="chbR"
                     /locus_tag="ACHKAU_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO5531342.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6211..6561)
                     /gene="chbA"
                     /locus_tag="ACHKAU_002284"
     CDS             complement(6211..6561)
                     /gene="chbA"
                     /locus_tag="ACHKAU_002284"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO5531343.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6612..7970)
                     /gene="chbC"
                     /locus_tag="ACHKAU_002285"
     CDS             complement(6612..7970)
                     /gene="chbC"
                     /locus_tag="ACHKAU_002285"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO5531344.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8055..8375)
                     /gene="chbB"
                     /locus_tag="ACHKAU_002286"
     CDS             complement(8055..8375)
                     /gene="chbB"
                     /locus_tag="ACHKAU_002286"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO5531345.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8674..9012)
                     /gene="osmE"
                     /locus_tag="ACHKAU_002287"
     CDS             complement(8674..9012)
                     /gene="osmE"
                     /locus_tag="ACHKAU_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO5531346.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9214..10041
                     /gene="nadE"
                     /locus_tag="ACHKAU_002288"
     CDS             9214..10041
                     /gene="nadE"
                     /locus_tag="ACHKAU_002288"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO5531347.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10271..11158
                     /gene="cho"
                     /locus_tag="ACHKAU_002289"
     CDS             10271..11158
                     /gene="cho"
                     /locus_tag="ACHKAU_002289"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO5531348.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11118..11693)
                     /gene="ves"
                     /locus_tag="ACHKAU_002290"
     CDS             complement(11118..11693)
                     /gene="ves"
                     /locus_tag="ACHKAU_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO5531349.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11896..12381)
                     /gene="spy"
                     /locus_tag="ACHKAU_002291"
     CDS             complement(11896..12381)
                     /gene="spy"
                     /locus_tag="ACHKAU_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO5531350.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12711..13679)
                     /gene="astE"
                     /locus_tag="ACHKAU_002292"
     CDS             complement(12711..13679)
                     /gene="astE"
                     /locus_tag="ACHKAU_002292"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO5531351.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13672..15015)
                     /gene="astB"
                     /locus_tag="ACHKAU_002293"
     CDS             complement(13672..15015)
                     /gene="astB"
                     /locus_tag="ACHKAU_002293"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO5531352.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKMHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15012..16490)
                     /gene="astD"
                     /locus_tag="ACHKAU_002294"
     CDS             complement(15012..16490)
                     /gene="astD"
                     /locus_tag="ACHKAU_002294"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO5531353.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16487..17521)
                     /gene="astA"
                     /locus_tag="ACHKAU_002295"
     CDS             complement(16487..17521)
                     /gene="astA"
                     /locus_tag="ACHKAU_002295"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO5531354.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17518..18738)
                     /gene="astC"
                     /locus_tag="ACHKAU_002296"
     CDS             complement(17518..18738)
                     /gene="astC"
                     /locus_tag="ACHKAU_002296"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO5531355.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19184..19990
                     /gene="xthA"
                     /locus_tag="ACHKAU_002297"
     CDS             19184..19990
                     /gene="xthA"
                     /locus_tag="ACHKAU_002297"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO5531356.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20157..20867
                     /locus_tag="ACHKAU_002298"
     CDS             20157..20867
                     /locus_tag="ACHKAU_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5531357.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20872..21549
                     /gene="ydjY"
                     /locus_tag="ACHKAU_002299"
     CDS             20872..21549
                     /gene="ydjY"
                     /locus_tag="ACHKAU_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO5531358.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21564..22271
                     /locus_tag="ACHKAU_002300"
     CDS             21564..22271
                     /locus_tag="ACHKAU_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5531359.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22271..22819
                     /locus_tag="ACHKAU_002301"
     CDS             22271..22819
                     /locus_tag="ACHKAU_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5531360.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22829..23995
                     /locus_tag="ACHKAU_002302"
     CDS             22829..23995
                     /locus_tag="ACHKAU_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5531361.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23968..25503
                     /locus_tag="ACHKAU_002303"
     CDS             23968..25503
                     /locus_tag="ACHKAU_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO5531362.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25503..26156
                     /locus_tag="ACHKAU_002304"
     CDS             25503..26156
                     /locus_tag="ACHKAU_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5531363.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            26223..27530
                     /gene="ynjE"
                     /locus_tag="ACHKAU_002305"
     CDS             26223..27530
                     /gene="ynjE"
                     /locus_tag="ACHKAU_002305"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO5531364.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27539..28159)
                     /locus_tag="ACHKAU_002306"
     CDS             complement(27539..28159)
                     /locus_tag="ACHKAU_002306"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5531365.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            28246..28653
                     /gene="nudG"
                     /locus_tag="ACHKAU_002307"
     CDS             28246..28653
                     /gene="nudG"
                     /locus_tag="ACHKAU_002307"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO5531366.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28619..28891)
                     /locus_tag="ACHKAU_002308"
     CDS             complement(28619..28891)
                     /locus_tag="ACHKAU_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO5531367.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29127..30470
                     /gene="gdhA"
                     /locus_tag="ACHKAU_002309"
     CDS             29127..30470
                     /gene="gdhA"
                     /locus_tag="ACHKAU_002309"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO5531368.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30587..>31498)
                     /locus_tag="ACHKAU_002310"
     CDS             complement(30587..>31498)
                     /locus_tag="ACHKAU_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5531369.1"
                     /translation="FYLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIV
                     RAGFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGK
                     DTFQYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEE
                     APEQVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIE
                     EELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDK
                     QFWEHYSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         7779 a         8293 c         7770 g         7656 t
ORIGIN      
        1 ttaaccattt taaaataaat aaacaattaa agaaaaaaga tcacttattt atagcaatag
       61 atcgtcaaag gcagcttttt gttactggtg gtttgaatga atgtagcaat gaaatacaga
      121 atttcaggtc atgtaactcc cggcaaaacc gggaggtacg taatctttac tcagtcactt
      181 ccccttcctg gcggatctga tttgcccaac gctgggcgga ttcaggcaca gtaaacgccg
      241 gtgagcgcag aaatgactct cccatcagta caaacgcaac atatttgcca cgcagcatcc
      301 agacatcacg aaaagaatcc atctttatcg catgttctgg cggcgcgggt tccgtgcgtg
      361 ggacatagct aataatctgg cggttttgct ggcggagcgg tttcttcatt actggcttca
      421 ctaaacgcat attaaaaatc agaaaaactg tagtttagcc gatttagccc ctgtacgtcc
      481 cactttgcgt gtatttcata acaccgtttc cagaatagtc tccgaagcgg gatctggctg
      541 atggtctata gttagagggt tttttgacca aaacagcggc cctttcagta ataaattaag
      601 gagacgagtt caatgtcgca acataacgaa aagaacccac atcagcacca gtcaccacta
      661 cacgattcca gcgaagcgaa accggggatg gactcactgg cacctgagga cggatctcat
      721 cgtccagcgg ctgaaccaac accgccaggt gcacaaccta ccgcccctgg gagcctgaaa
      781 gcccctgata cacgtaacga aaaacttaat tctctggaag acgtacgcaa aggcagtgaa
      841 aattatgcgc tgaccactaa tcagggcgtg cgcatcgccg atgatcaaaa ctcactgcgt
      901 gccggtaacc gtggtccaac gctgctggaa gattttattc tgcgcgagaa aatcacccac
      961 tttgaccatg agcgcatccc ggaacgtatt gttcatgcac gcggatcagc agctcacggt
     1021 tatttccagc catataaaag cttaagcgat atcaccaaag cggatttcct ctcagatccg
     1081 aacaaaatca ccccagtatt tgttaggttc tctaccgttc agggtggcgc tggctctgcc
     1141 gataccgtgc gtgatatccg tggttttgcc accaagttct atactgaaga gggtattttt
     1201 gacctcgttg gcaataacac gccaatcttc tttatccagg atgcgcataa attccccgat
     1261 tttgttcatg cggtaaaacc agaaccgcac tgggcaattc cacaagggca aagtgcccac
     1321 gatactttct gggattatgt ttctctgcaa cctgaaactc tgcacaacgt gatgtgggcg
     1381 atgtcggatc gcggcatccc ccgcagttac cgcaccatgg aaggcttcgg tattcacacc
     1441 ttccgcctga ttaatgccga agggaaggca acgtttgtac gtttccactg gaaaccactg
     1501 gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaac tcaccggacg tgacccggac
     1561 ttccaccgcc gcgagttgtg ggaagccatt gaagcaggcg attttccgga atacgaactg
     1621 ggcttccagt tgattcctga agaagatgaa ttcaagttcg acttcgatct tctcgatcca
     1681 accaaactga tcccggaaga actggtgctc gttcagcgtg tcggcaaaat ggtgctcaat
     1741 cgcaatccgg ataacttctt tgccgaaaac gaacaagcag cattccatcc aggtcatatt
     1801 gttcctggtc tggacttcac caacgatccg ctgttgcagg gacgtttgtt ctcctatacc
     1861 gatacacaaa tcagtcgtct tggtgggccg aatttccatg agattccgat taaccgtccg
     1921 acctgccctt accacaactt ccagcgtgat ggcatgcatc gtatggggat cgacactaac
     1981 ccggcgaatt acgaaccgaa ctcgatcaac gataactggc cgcgcgaaac accgccggga
     2041 ccgaaacgcg gcggttttga atcatatcag gaacgcgtgg aaggaaataa agttcgtgag
     2101 cgcagtccat cgtttggcga atattattcc catccgcgtc tgttctggct aagtcagacg
     2161 ccattcgagc agcgccatat tgtcgatggt ttcagttttg agttaagcaa agtcgttcgt
     2221 ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata ttgatctcac tctggcccag
     2281 gcggtggcga aaaatctcgg tatcgaactg actgacgacc agctgaatat caccccacct
     2341 ccggacgtca acggtctgaa aaaggatcca tccttaagtt tgtacgccat tcctgacggt
     2401 gatgtgaaag gtcgcgtggt agcgatttta cttaatgatg aagtgagatc ggcagacctt
     2461 ctggccattc tcaaggccct gaaggccaaa ggcgttcatg ccaaactgct ctactcccga
     2521 atgggtgaag tgactgcgga tgacggtacg gtgttgccta tagccgctac ctttgccggt
     2581 gcaccttcgc tgacggtcga tgcggtcatt gtcccttgcg gcaatatcgc ggatatcgct
     2641 gacaacggcg atgccaacta ctacctgatg gaagcctaca aacaccttaa accgattgcg
     2701 ctggcgggag acgcgcgcaa gtttaaagca acaatcaagg tcgctgacca gggtgaagaa
     2761 gggattgtgg aagctgacag cgctgacggt agttttatgg atgaactgct aacgctgatg
     2821 gcagcacacc gcgtgtggtc acgcattcct aagattgaca aaattcctgc ctgatgggag
     2881 gcggcgcaat tgcgccgcct caaatgattt acatagtgcg ctttgtttat gccggatgcg
     2941 gcgtgaacgc cttatccggc ctacaaagtc gtataaattc aatgtattgc agtttattgg
     3001 tagatactga taagcgaagc gcatcaggca gttttgcgtt tgtcagcagt ctcaagcggc
     3061 gcagttacgc cgcctttgta ggaattaatc gccggatgca aggttcacgc cgcatctggc
     3121 aaacatcctc acttacacat cccgataact ccccaaccga taaccacgct gagcgatagc
     3181 acctttcaac gacgctgatg tcaacacatc cagctccgtt aagcgtggga aacagtaagc
     3241 actctgacgg atagtattat cgataaacgc cggatgacac atcacctcca gcgaacgatc
     3301 accccgatgg cttgcgtcat ccagcacttg caggaacagt gcctcgctaa tctcttcacc
     3361 ataaaacgcg ctactgaatc cctgagaact gcgcagatta gccggtaaat caccagcgtt
     3421 agatagcggc tgacggtcga tgcgcaacgc aatcccctct tcagccgcaa acctggcaac
     3481 aatcgggaaa atctgcggga acatatgcac atgatgatgg ctatcaagat gcgtaggttt
     3541 gcgtccaaat agctcaatga aacgcaaata ctgactggca agctcctgag taatttcttc
     3601 cagcggtaaa gcatcttctt ctgccaactg ccagatccat tttcccagca caccatcgcg
     3661 ggttaacccc ggcatagctg tcaggggctt gcccatagta aggacaaagt gcatccctat
     3721 ggccagactc ggttcatcac gactcaactg caccgcatgg tcaatagcct gcccattcac
     3781 cagcgccgtc gttgatgtga caatcccatt gcgacaggcc tcgataatgc cgtagttctg
     3841 acctttgctt aagccaaaat catcggcatt aacaatcagt aagcgttcca taatcagcct
     3901 cggttaatgt gcttttttaa gctctgcgat gcagtcggca aaatttggca gccatttctc
     3961 gtgcgccaga atcatctcgc gtgccagcag ctcagcatcg cgatcggaat gcaccaacgg
     4021 actaaggttt agcgccagta acacatcgtt aaattctccg ctaagtgcgg cgttgctggc
     4081 agcaatctcg aagcctttaa tggtgtgaat cagccccatc actttatcat cgaaatgcgt
     4141 aatgcgcgga tgtggcgtcg cgccatcgcg ccccagctta caggtcattt ctaccgccca
     4201 gtctgccgga atattatcaa tatgcccatg atgcgggata ttaacgtaat gttcagcttg
     4261 cttgtcgttg tagatagcgt tgatcacttc gcacgctgca tcagagtaat aagccccacc
     4321 gcgctgttcc agttctttcg gcttaacttt caactccgga tttttataca gctcaaaaag
     4381 ttgtttctcg actttctgta ctacctgtgc tcgtgcgccg cctttgtagt attcgcccat
     4441 ttcaatagcc agcatctctt tctgcttgaa gtaatacagc agataagaac atggcagcag
     4501 atttaacgaa cgaattaagc cctcactaaa tggcagatcg aaaatatttt ttacagagga
     4561 cgcttttaac tgccctgacg ccacaccatc aagcaattcg gcaaagcgcg acttgccatt
     4621 taccagcaca tccttaatga acaccatatg gttgaggccg aacagatcga tagataaatc
     4681 atcgctgtct ttcagcatca gaacatcgcg aataaacatc ttcatgccga tcggaatatt
     4741 acacacgccg ataaagcgtt taaatccggt atgacgataa acggcttcag tgaccattcc
     4801 cgccgggtta gtgaagttaa tcacccatgc attcggacaa agttcttcga catcttttac
     4861 gatgtcaaaa atcaccggaa tggtacgcag acctttaaac agaccgcccg cgccgttggt
     4921 ttcctgacca agataaccat gacttaatgg aatacgttca tccagttcgc gcgccggtaa
     4981 ttggcctacg cgcagttggg tagtaacgaa atcagcatct ttcaatgctt cgcggcgatc
     5041 cagcgtttta taaagcttca tcgggacgcc agcgttatca atcatccgtt ggcagagttc
     5101 aaaaatgata tccagtttct ctttaccatc ttcgacatcc accagccata attcgctgac
     5161 cggcaattcg tgataacgct taataaatcc ttccagtaac tccggggtat agctgctccc
     5221 gccaccaata gtgacgactt ttaatttctg gctcataatt tctcccttca gtacagaata
     5281 ctgatatctg gcatatctgc ccccccggac ataaataatc cagcaacagg acagatatgt
     5341 gaattgtcag gtataacgac ttactgatta aattcagtca atttcttacg atagctctta
     5401 ggcgtaaagg acgttaattt cttaaacgtt ttaataaaca aactcgggct actataaccg
     5461 gcctcaaacg caatatccgt cacggaatag ttggtcattt ccagttgttt tttggcaaaa
     5521 ttaatacgga tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtggct
     5581 cgcgtcaaat attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca
     5641 ctaaactgct ctttatcatg catcttttct accgtacttt tcagccactg cggtacatca
     5701 tcaatcacct gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc
     5761 tcaacaaact cttccagccc tgtttcgcgg aaattcaatg aagaaatcac tgtttccaca
     5821 taggtgagaa aagcgttatt ggtccggtat acctgcgaag cgacaaagca ataaggcaac
     5881 aacggcaggt aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatacgcgtg
     5941 gcaccaaact cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc
     6001 cgttccagta acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga
     6061 gtaaattcat aatagtcgtg ctgatgcagc ccgctgatac tctcagtttt gttatagata
     6121 aacacatgga agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc
     6181 gcgttaatca ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat
     6241 taattcagta atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac
     6301 cagcaccaga ctcactttca tcttaacttc gcccgcatcg ccttcaatca gtttcgtctg
     6361 taccagatgc gcttcattca atgccatccg tgactgatcc atcatggctt ttgctgcagc
     6421 aaaatcgccc tgcttcgcct gtttcagtgc cgcatacgcc aggctgcgcg cttgtccgga
     6481 gttgatgata agccccatca ccacttcttc cagctcttca gcttctgttt gcgtatcagg
     6541 aatattatcg agatccatca tacatcgttc ctctctcttc ttaccggcaa gattacccgt
     6601 accggcatcg attaaaattt cagggcgtta gcgatatctt cttcgctctc ttctttatca
     6661 atcgcatttt gtgctttgtt agccaccaca acaaagggca gataaattaa ggttgcgatg
     6721 ccaaggttga agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct
     6781 cccagaccgg ttggcatggt ccacggtgca atattggtca ccggaggaat gatgcccatg
     6841 tagtacgctg cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac
     6901 atcaccgggt tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg
     6961 ccggacggca gcgccagttt tgccacctga cgataatcag cacggcgaga ggcgataaag
     7021 atagccagga tcaggcctaa agtcgcacca ctgcccccaa ggaaaataaa ggagtccagc
     7081 atcggcttgg cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca
     7141 tattgctgat aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt
     7201 gcggtcagcg ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca
     7261 taggcccagc ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg
     7321 atctgatgga agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa
     7381 agaataataa agccgggaat taatgccgag aaggaacgcg atactgaagc aggtacgctg
     7441 tcgggcagtt taatgaccca attgcggcgg acaataaagg taaacatttc tgccaccacc
     7501 aggccaataa taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca
     7561 taggcctcac cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaacccc
     7621 gccgccagcg catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc
     7681 attaaagaca ttatccctaa tgttccgtta tatacgttgc cgccaatacc tttcaggcca
     7741 ttaagtgttt caatggttga ggcgtcgagg cgaataccta aggaataaaa aaacgacccc
     7801 tccccaaagc ttagaaaaac gttgttaatt aatacaaaca tggcccccgc aagggttaac
     7861 ggcattaagc gaataaagcc atttttgatt gcattaacgt gtggctgctt tcctatttta
     7921 actgcaaaag ggaggagtac cttttcaagc gatgcaataa cattactcat agaaaaatac
     7981 ccttaaaaac cgcaatttaa atattgcggt attgatttat gaaataactc tttgacggga
     8041 aaatttaaaa taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc
     8101 acgcctaaac catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg
     8161 ggtaacaaac gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca
     8221 tcggcattct gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga
     8281 acttcatatt tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt agacatgccc
     8341 gcagaacaaa acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc
     8401 tgtcagccag acactccgca agccttaacc tgcttccatg ctctgggtaa cttgcgaaac
     8461 caacatgact ttttttgtgg ctgaaaggag tatacggtat agacctgagc aatagtattt
     8521 ctttgacctt ataaattgtt tctccttgac ctgtggttat gaccctcttc acatttcggg
     8581 caaataattc gcgaagataa ttaagaactc aggccaaaaa aaaccggcgc aatggccggt
     8641 ttccgttgtt actcaagctt tcagacgaat tgattacttc gcagcctgtg gatcagtgtc
     8701 gtattcagca caggtctgat aaccggagtt gatgacatgt ccggtatcat ctaacgcgac
     8761 aaagtaggtt tctgctttac catcacgttg acccaggatg taggtctggc aagtaccgcg
     8821 agcatggatc atgctcactt cagacgaagg tttacccgca atttgtgcaa cctgcgcccg
     8881 gctcatgcct tttttgacgt ctttcaccac aggctgtaca aactggtctt tggtacgatc
     8941 ataagccgta caacccgcca gcatggttaa taccgccgct gcactcagaa ttcctgccat
     9001 attcttgttc atattccgtc ctcttgttta tcagcgtgtt agataagcct ggaatacatt
     9061 gggcgctttt tcaagcccgt gaacgaaacg gctccgcttt cagaggattc ctgtatgacg
     9121 ttttaaccac cattcagccc gctgtcgctt gtcgtttcag tagcaacggg ttagctttaa
     9181 ggaagttttg tcttttctgt ctggaggggt tcaatgacat tgcaacaaca aataataaag
     9241 gcgctgggcg caaaaccgca gattaatgct gaagaggaaa ttcgtcgtag tgtcgatttt
     9301 ctgaaaagct acctgcaaac ttatccgttc attaaatcac tggtgctcgg gatcagcggc
     9361 ggtcaggact ccacgcttgc cggaaagctg tgccagatgg cgattaatga gctgcgccag
     9421 gaaactggca acgaatcact gcaatttatt gccgtacgcc tgccctatgg tgttcaggct
     9481 gacgaacaag attgccagga tgccattgcc tttattcaac cggatcgcgt attaaccgtt
     9541 aatatcaagg gcgcggtatt ggctagcgag caggcattgc gggaagcagg cattgaactg
     9601 agcgattttg tccgtggcaa tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt
     9661 gcgggtatga ccagcggtgt cgtggtgggc accgatcatg cagcagaagc cattaccgga
     9721 ttcttcacta aatatggtga cggcggtacg gatattaatc cgctgtatcg tctcaacaaa
     9781 cgccagggta aacagttgct gacggcatta ggttgcccgg aacaccttta taagaaagcc
     9841 ccaacggccg atctggaaga tgatcgccct tctctgccag atgaagtggc actcggcgtg
     9901 acctatgaca atatcgacga ctatctggaa gggaaaaacg tacctgaaca ggtcgccaga
     9961 acaatagaga actggtatct gaaaaccgaa cataaacgcc gtccgccaat taccgttttc
    10021 gatgatttct ggaaaaagta ataatttgca cataatggtt tgtgcgaaaa agagtaattt
    10081 gttcacgccg gatgcggcgt gaacgcctta ttcgacctac aaaactatgc aaattcaata
    10141 tattgcagga gtcgaatagg cctgacaggc gtagcacgtc agacggtgta acctttgtca
    10201 tcgacccgcc tcttttttaa tcgcttcccg cctgttacac tggatagata accagcattc
    10261 ggagtcaaca gtggtacggc gtttaacttc tccgcggctc gaatttgaag ctgcggcaat
    10321 ttatgaatat cccgaacatt tacgttcatt ccttaatgac ttacccaccc gaccaggggt
    10381 gtatctgttt catggcgaaa gtgacaccat gccgctctat atcggcaaaa gcgttaatat
    10441 ccgcagtcgg gtgctttctc atttacgcac cccggatgaa gccgccatgc tacggcaatc
    10501 ccggcgaatt agctggatct gcaccgcagg cgaaatcggc gctctgctcc ttgaagcgcg
    10561 attaatcaaa gaacaacagc cgctgtttaa taaacggttg cgccgcaatc gccagctctg
    10621 tgccctgcaa ttaaatgaaa agcgcgtcga tgtggtgtat gccaaagagg tggatttttc
    10681 acgagccccc aacctgtttg gcctgtttgc caataggcgc gcagctttgc aagcattgca
    10741 gagcatcgct gatgaacaaa aactttgtta tggcctgctg ggactggaac cgttaagtcg
    10801 cggtcgtgca tgttttcgtt cagcgctaaa acgttgcgcc ggagcatgct gcggtaaaga
    10861 gagccatgag gaacatgcgc tacgcttgcg ccagtctctg gagcgtttgc gggtggtgtg
    10921 ttggccttgg caaggggcgg tggcgctgaa agaacagcac ccggaaatga ctcaatatca
    10981 tattattcaa aactggctgt ggctgggggc ggttaattcg ctggaagacg cgacaacgtt
    11041 aattcggaca cccgccgggt ttgatcacga cggttataaa attctttgta agccgctgct
    11101 ttccggtaac tatgaaatta ctgaacttga tccggcgaat gaccagcgag ccagttgatt
    11161 tcactgaaca acagtttacc ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac
    11221 cagcacgcgc cctggtcggt agtgagcagt ttgtcgccca gttgccacgc cccgttaatc
    11281 acaaacacta ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc aattctgact
    11341 ttcgccttac agacatccag gcgcgtcata atattgaaat ccatcgacat ctgccctgcc
    11401 gtcagtttcg cttttaccac ctggtccgcc gcaaaggcaa aaggctgtaa cggttttaag
    11461 gtatggttaa agtggtctgc gctttcaagg aacatctcgc cgccttccag caacgtcact
    11521 atcctttcca taccgggaaa caaagaaaac tccccattcg ccgcgatgga tgcaatgctg
    11581 gcacgccagt aaaaatcacg ttttgccggt gggaaagtgc aaatttcgcg cgtttcaccg
    11641 gcagcatttc gccacagatt caccgacatt ttacgcatat caaagtattc catactcgct
    11701 cccggcccgt tcttttcatt gttatcgttc aggtaaccgt ggatggttat cggcaactcg
    11761 ggtgatttac gttagtggtg atcaggcttt ttttacaact gtttgagaag agaaaagaaa
    11821 accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt
    11881 tttttggctg aaagattatt cagcagttgc aggcatttta ccttttgccg ctggacgttc
    11941 tgtcagacgc ttctcaaaat tagcattaaa ttgctttttc tgttccggcg tcaggatgtt
    12001 gtaaattttg ttctgggttt ccatgtgcgc cagcatgtta gctttgcgct gttcttccat
    12061 ttttgcgatc tgcgcttcag cttttgcttt atcgaaggta tcgctggcaa cgatgtcatg
    12121 cattgcgcgg cgttcttcca gcggcggacg tttcatctgg tcacgctggc ctttcatgat
    12181 ttcgcggatc tgctgtttct gcgcgtcggt caggttcagg tctttgaaca tcatgtcctg
    12241 atgcggaccg aacttgcctt tgtggtgcat aatcggcttc gcgtcagccg gtgctgcggt
    12301 agtggtgtct gcggcatggg ccaggttagc cgcgccaaga gccagggtag aggcaacaaa
    12361 cagtgcagtt aatttacgca tattctatat ccttcctttc agttatttat tacggctttc
    12421 ttaagtagcg tgccgtgttg acgagattaa ctttactgac tttagcgtca attaatcaga
    12481 gcaacggtaa aacaatgaaa gtgtaaaaaa cacttttgcg ccaattatgg agaaaaaaag
    12541 aaaatttgat ggagagtgat gagagaatat tacaacacga tgattttgca gagattatga
    12601 agaactatac cggatgactg gtgataaata aagcaaataa ccaggattaa tctgtattaa
    12661 tttataagaa agcaacttaa tacccgcaga gtgatttctg cgggtaagta ttagcttatt
    12721 ttttcgagca ttaatcccgc gcgtaatccc aacgctacca acggattagg gaataacaca
    12781 tactctacat catgggttac ggtaaaacgt tcctctccgt cctgcgccag caatgttcct
    12841 ttctcaaacg gcataaaatt cagcgtgtca cttgccatat gcatttcgaa ggacggcgag
    12901 tggcgagtaa tttgcgaaac cacccgataa cggagcggcg gtgttctcac gataccgaca
    12961 ctctcaccag atagcagcgc agcaattgcg ctggcagtta ctgcaaactg gcgaagatcg
    13021 ttttgcccaa agggcaacgc tttgccaagt tccagcgtac aggccagcgc gccaaaatgt
    13081 ctggcgctga aatgggtaaa cgtaccacca ggttcctgat ggaacaccag cgcctccagc
    13141 cccgccgcac ccagccacgt cagaaatttc tcgtcccagg gaatgtcgcg ttgcggtaat
    13201 acaccgaact gcggatgcaa ggagccacga attgcggtat gtagatcaag gtgccagcgc
    13261 acagattctt tgccctggtc ataaaaatct tccaggcact gttccagttc gcgcgcccga
    13321 caggtttctc cgctttcagc aaatagctgc caacgaccgc caaacattcg attcatatcg
    13381 ctatggcaat aacgtttccc ttgcttcagc gcaggaggat tcccgaggat caccagcaac
    13441 cgccaacgta acgggatctc gccgtgagat atcgcgccaa gcaacgcgtc cagcatctcc
    13501 acaggtgccg tctcattacc gtgtattccc gctgaaatca ccagtgcgcc ttgcggtggc
    13561 gttaatggcg tcagttccag cacaccatcg cccagccagc gccagcgaac gccgttgatt
    13621 tctcgctcgg tgataaccgg ttttttaccc gttaaggtca gagcaagaaa attatccatt
    13681 gccccctccc tcgcgctgga acggataaac cgaaccgaga ttcagtaatt ggctcaatac
    13741 atccagtgct tcccgccctt cgcgcagcaa ttgcgggtcg gccagatcgg cagcagtaag
    13801 gcgatcgcgg tagtaacgat ccacccagtc attgagcgca ttaaacagcg tatcgttcat
    13861 catcaccgcc ggattcaccg cccggcgttc ttcttctgtc aataccaccc gcaaccgcag
    13921 gcacgccggg ccgccgccat tcgccatgct ttcacgtaaa tcaaagactt ttagttcgct
    13981 aatcgggttg tcagcggcaa ggagttcatt gagataaccc catactccgg cgtgttcccg
    14041 acactcctga ggcagcacca acatcataga accatcatcg cggctcaaca gttggctgtt
    14101 aaacagatag gtagacaccg catccgacac ggaaacctga gttgccggaa cttctatcgc
    14161 cataaaaccg ttcacccgcg cacgcaggtt tgccagtaac tgtgactggc gagcgaacgc
    14221 ctgttggtgg caaaacagca cctggcggtt actcacggca atcacgtcat tatgaaaaac
    14281 gccctggtcg ataacgtccg ggttttgctg ggcgaaaatc acctgttggg gattcacctg
    14341 attcagcctt gccaccgcct cgctggcttc gcgagtctgt cgcgccggat aacgggaagg
    14401 ccgggtatca ttgccttctt ctcgcccgta gacaaaaagt tgcatacccg gctcaccgta
    14461 atgaccgccg agacgattgt ggtttgccgc cccctcatca ccgagcaacg ctacctgcgg
    14521 caacgccgaa tgaacgctaa atttctcttc gtcgttaaaa atcgctttta aaagtgattc
    14581 ggtgacgtgc gcttccagcg agcggtgaaa tttattgttc aggttggcaa cggtgagatg
    14641 cattttgcca tccagcgtat cggcagatgg cgcgatcgtt gccgcattgg ctacccacat
    14701 tggcgaagcg gaactgacgc tggaaagcca gtgcggtgcc tggcgtgcaa ctttttccag
    14761 tacctgctca tcgctaccac tgaatcccaa ctgacgcagc accggaataa acggacgctc
    14821 gtgcggcggg atcacggcct gggggaatcc cgcatcggca agggctttca ttttcagtaa
    14881 gccctgtttt gccgcctgtc gcgggttaga cacctgaaaa cggtgacggg tagaggcttc
    14941 attaccaaac gacaggccag cgtaatgatg cgtcagccct accagcccgt cgaaattaac
    15001 ttcccaggcg ttcatcgcac cacctcatcg gaaaaatcca gcccggggtt aagcgttgcg
    15061 ggcaaagtta acgagtccga ctccaggctc gccataggcc atgcgcagta atctgcggca
    15121 taccaggcgc tggggcgatg gttaccggat gcaccaatgc cgccgaatgg cgcggtactg
    15181 gcagcaccag taagcggttt gttccagtta acaatccccg cccgcgcctc cagcaacagt
    15241 tgatcgaatt tttcccgctc gggggaaacc agaccgcaag agagtccgaa gcgagtgtta
    15301 ttcgccatta gaatcgcttc ctcgaaagaa tcataacgcc agacgcgcaa taacggtccg
    15361 aacacctctt catctggtac gccagcaacg cctgtcattt caatgatccc cggcgtcagc
    15421 aacgatgtct ctgattgtaa taagcgcggc gcaagcaggg ttcgtccgcc catcgcttcc
    15481 agttgctgcc aggcagtaac cacctgctgt gcggcctgtt cagaaatcag cccgccaata
    15541 aacggctgcg gttcgtcatc ccagttgccc ggcgttaatc gctggctgac ggcaaccaga
    15601 cgagcaagaa acgcatcgcc ctgcgctccg cttttgagca ataaacggcg ggcgcaggtg
    15661 cagcgttgcc cggctgtgac aaacgccgac tgaatggtca gatggacagc cgcgtcgata
    15721 tccgccacct catcgataat tagcggatta ttaccgccca tctcaagggc gagaattttc
    15781 tccggctgac cggagagctg gcgatgcagc tggtagccgg tattggcgct accggtaaac
    15841 agcaaaccgt cgagatcctc cagcgcactc agcgcctgac ccgtttcacg cccgccctgc
    15901 accaggttca gtacgcccgg cggcaagcca gcctgctgcc ataaacgcat taccgcttcg
    15961 ccactccacg gtgtcagttc gctgggttta aagataatgg tgttacctgc cagcaatgcc
    16021 ggaacgatat gtccgttcgg caaatgacca gggaaattat acggcccaaa caccgccagc
    16081 acgccgtgcg ggcgatgtcg caggctcgcc gcaccgtccg gcatttcact acgctgctcg
    16141 ccggtacgaa cgtgatacgc cttaattgat atcgcgattt tattgatcat cgccgtcact
    16201 tcggttgccg cttcccagcg cggcttaccc gtttctctgg caataatcgc ggttaattcg
    16261 cctttattcc tttccagcaa tccggcaaag cgttcgacaa cgacctgacg ttcagccaat
    16321 gagagccgcg cccagcgcgg aaacgccgca cgggctgccc gacaagcctg ttcgacctga
    16381 gcggcatcgg catcattgcc ttgccataac acctcgcccg ataccggatt acgcttcaca
    16441 cgcgatgcgc cctggcccgt tatccagtca ccgttaatcc ataaagtcat gctgttttct
    16501 cctctgcgca caggcgcacc agacgaacgc gatcccctgc gtggcatttg agggcatcca
    16561 gttgtgcggc ggttaaaatc aaacgctcgg ttgccggatc ggtacgcgcc agcaccacgc
    16621 ggaaatggtg ataattttca ttggcgacca ggcaggccgg aaaatcgccc tgcgcaggct
    16681 gcccttctgc cacttccacc agccgacttt tacgaatggc gcgcacgcgg tcgatgtcac
    16741 actcaagcgt tggcccgccg tcgaagatgt cgatatagtt gcggtaacga aaaccttctt
    16801 tctccagcac cgcgcgggca ggcgcggttt gcggatgtac ctgaccgatg acgtcctggg
    16861 cttcctggga taaaaagtgg gtatagatcg gatgtttcgg catcagttct gcaataaatg
    16921 ccttttgccc ggtgccgcag agaaaatcgg cgcggctaaa atccatcgaa aagaagcgtt
    16981 taccgaggct ttgccagaac ggtgaatagc cgtgttcgtc aatcaccccg cgcatttcag
    17041 caaccacttt gtcattaaac ttgtcgcgaa aagccgccat aaacataaag cgcgatttcg
    17101 acagcaaata gccgttgccc tctttgcgcc agtccgggtc gagaaacagc gtgcacagct
    17161 cgctgctgcc ggtgtgatcg ttactgagaa acagcgtcgg caatgcgtta tagacattca
    17221 gctcttttga ggcgtgaacc aacgtgccga cgcgatagtt gtaccaggga tcgttcagcc
    17281 caaccgccac ctcaatggca caaatccccg ccacggtgcc tgtctcgcta tcttccagca
    17341 cgaacacata gccctgctca cttttgggca gttcgccttg ccaggttttg attgcccttt
    17401 cgatacgcgc cgaaagcgtg gcttcattgg cgggaagcga cgtcaggccg ccgcccgttt
    17461 tgctggcaag ctgcatcagc gccgagacat ctgatcgctc aacgggacgg atgaccatca
    17521 tgatgaacct cggctaacaa agtgttcgca agcagctgca aagcgatcca gtccggtcgt
    17581 cacctcttct tcgctgacat tgagcgcagg cgcaaaacgc accacgttgc cacccgcaat
    17641 cagtaccatc acgcctgctt tcgccgcttc ctgagagatc tgtttcgctt gcccggcgta
    17701 atcggcattc agtacacagc caatcagcaa acctaagccg cgaacttcac tgaacaaacc
    17761 atagcggtga ttaatagtat taagacgctc aacaaaccag tcgtgacgct gtttaacgcc
    17821 attaagcatc tctggtgtgt tgatgagctc cagcactttg cctgccaccg ccgaggccag
    17881 cggattaccg ccataggtgg tgccatgagt gccaacggtc atcacgcggg cgcactcttc
    17941 ggttgccaac aacgcaccga ccgggaaacc gccgcccagc gctttggcgg tagttaacag
    18001 atcaggcgtc acgccgtagt gcatataggc atacagttcc ccggtgcgcc cgacgccggt
    18061 ttgtacttca tcaaaaatca acagcgcatt gtggcggtta cacaattcac gcagaccttg
    18121 taaaaacgcg ttgctggctg gcaccacacc gccttccccc tggatgggtt cgacaatcac
    18181 cgcacaggta gagtcgtcaa tcagcgcgct ggcagagtta atatcgttat atgcagcatg
    18241 acgaatatcc gccggcagtg gcgcaaaatc ctgtgaatag gctggctgcc cacccgcact
    18301 gacagtaaac agcgtgcgac catgaaacgc atttttgaac gccacgatgc cgctcttatg
    18361 gctgccgtag cggtcgtgag cgaatttacg cgccagtttt agcgccgctt cgttggcttc
    18421 cgcaccggag ttacaaaaga agacgcgatc ggcaaacgtg gcgtcgatca attttttcgc
    18481 cagtcgcagt accggctcgt tggtgtaacc gttgccggta tgccagaact tactcgcctg
    18541 ttcgttcagc gcttcacgca gttccggatg cgcatggccc agcgcgttca ccgcaatgcc
    18601 acccgcgaag tcgatatact ctttccccag ctgatcccac aagcgcgaac cttcgccacg
    18661 taccggtata aagggtgccg gagcgtaaac aggtatcatc cattcatcaa agttttcacg
    18721 cgtaattggc tgagacatag cgacctctac agtaaataat tcgttattta tatgttaaca
    18781 ataagtaatg tttgcgctgt aaatgtagat tgcagggttc gtgccagcca gtgataaaag
    18841 tgcataaacg gcggaggcta actggaaatc aaggagttat aaccaaacca tatgcattta
    18901 aagtgcatat aaagtgaata cgtttgcgat gtgggtgaat aaaaagaata aaaaacgcaa
    18961 tgttatgcag aagtaaaata taattctgga attgtgatca ttgacgaaat ttactggaaa
    19021 ttactgcgcc attctgacgc agcgcgcacc aaaagcgggc attttttgcg ccatcgttga
    19081 catcattaac aaccatcgat caaatcactt aacaacaggc ggtaagcaac gcgaaattct
    19141 gctaccatcc acgcactctt tatctgaata aatggcagcg actatgaaat ttgtctcttt
    19201 taatatcaac ggcctgcgcg ccagacctca ccagcttgaa gccatcgtcg aaaagcacca
    19261 accggatgtg attggcctgc aggagacaaa agttcatgac gatatgtttc cgctcgaaga
    19321 ggtggcgaag ctcggctaca acgtgtttta tcacgggcag aaaggccatt atggcgtggc
    19381 gctgctgacc aaagagacgc cgattgccgt gcgtcgcggc tttcccggtg acgacgaaga
    19441 ggcgcagcgg cggattatta tggcggaaat cccctcactg ctgggtaatg tcaccgtgat
    19501 caacggttac ttcccgcagg gtgaaagccg cgaccatccg ataaaattcc cggcaaaagc
    19561 gcagttttat cagaatctgc aaaactacct ggaaaccgaa ctcaaacgtg ataatccggt
    19621 actgattatg ggcgatatga atatcagccc tacagatctg gatatcggca ttggcgaaga
    19681 aaaccgtaag cgctggctgc gtaccggtaa atgctctttc ctgccggaag agcgcgaatg
    19741 gatggacagg ctgatgagct gggggttggt cgataccttc cgccatgcta atccgcaaac
    19801 agcagatcgt ttctcatggt ttgattaccg ctcaaaaggt tttgacgata accgtggtct
    19861 gcgcatcgac ctgctgctcg ccagccaacc gctggcagaa tgttgcgtag aaaccggcat
    19921 cgactatgaa attcgcagca tggaaaaacc gtccgatcac gcccccgtct gggcgacctt
    19981 ccgccgctaa tttagcagct ctcctggctc aaactgggtc aggagaatta accttgagaa
    20041 aaatcaacaa actgtcagta atgatttgtt gcctgccgtc ctttgttata ccgtcactgc
    20101 gtttttagtt gtctgaccac ttctctatta tcaagtttga tataggaaac tccacgatga
    20161 acgctgagcg taactttctg tttgcctgtc ttatttttgc gctggtcatt tacgctatcc
    20221 acgctttcgg tttattcgat ctgctcaccg atttacccca cttacagaca ctcattcgcc
    20281 agagcggatt gttcggttat agcctctata ttctgttatt catcattgcc accctctttc
    20341 tgttaccagg aagcatactg gtgatcgctg gaggaatagt ttttggcccg ctcttaggga
    20401 cactactctc attaattgcc gcaacgctgg cctcctcgtg ctcattcctg ctggcgcgct
    20461 ggctgggacg tgatttactg ctgaaatacg tgggccatag ccataccttc caggccattg
    20521 aaaaaggcat tgcgcgtaac ggtattgatt ttcttattct gacccgctta atcccgttgt
    20581 ttccttacaa tattcaaaat tacgcttacg gattaaccac catcgccttc tggccttata
    20641 cccttatttc ggcactcacg accctgctcg gtattgttat ttataccgtg atggcaagcg
    20701 atctcgccaa tgaaggcatt acgctgcgct ttattttaca actctgtctg gcgggcctgg
    20761 cgctgtttat tctcgtccag ctcgcaaaac tctacgcccg acacaaacat gtggatctgt
    20821 ctgcttcgcg ccgcagccca cttactcacc caaaaaatga aggatagaac gatgtcgcaa
    20881 cattattcag tgtcatggaa aaaaggactg gctgcactct gcttactggc tgttgccggg
    20941 cttagcggct gcgatcaaaa agagaatgcg gcggcaaaag tggaatacga cggactttcg
    21001 aacagccaac cgctgcgcgt cgatgccaat aaccatacgg taaccatgct ggtgcaaatt
    21061 aatggtcgtt tcctcaccga cgacactcgt cacggtattg tgtttaaaga tggctccaac
    21121 ggacataaat cgctgtttat ggcttatgcg accccgaaag cattttatga agccctgaaa
    21181 gaggcaggtg gtacgccggg cgaaaacatg acgatggata ataaagaaac gactcatgtc
    21241 acaggcagca aactggatat ttcggtcaac tggcaagggg cggcaaaagc gtattccttc
    21301 gatgaagtga ttgttgacag taatggcaag aaactggaca tgcgctttgg cggtaattta
    21361 acggcagcag aagagaagaa aacgggttgc ctggtgtgtc tggatagctg cccggtcggc
    21421 atcgtcagca atgcaacata cacttatggt gcggtggaaa aacgcggcga agtgaaattc
    21481 aaaggcaatg cctcagttct cccggcggat aacacgctgg caacggttac ctttaaaatc
    21541 accgaataaa gccaggataa aggatgatga agatacaatc gcgtaaaatc tggtactacc
    21601 gtataaccct catcatcctg ttgttcgcta tgctgctcgc atgggcgctg cttcccggcg
    21661 tccatgagtt tatcaatcgc agcgttgcgg cgtttgccgc cgtggaccaa cagggtatag
    21721 aacgctttat tcagtcttac ggcgcactgg cggcagttgt ctcgttcttg ttgatgattt
    21781 tacaggccat tgctgcaccg ctacctgcgt ttttgatcac ctttgccaat gcgtcgctgt
    21841 ttggcgcgtt ctggggcggc ttgctgtcgt ggaccagttc gatggccggc gcggcgctgt
    21901 gcttttttat cgccagagtg atgggccgcg aagtggtgga aaaattaacc ggcaaaaccg
    21961 tgcttgacag tatggacggc tttttcactc gctacggcaa acacaccatc ctggtctgtc
    22021 ggttattgcc ttttgtccct ttcgatccaa tcagctatgc tgccggtctg acttcaatac
    22081 gttttcgctc gttttttatc gccaccgggc ttggtcaatt accggcgact attgtttatt
    22141 cctgggcggg cagcatgtta acaggcggta ctttctggtt tgtcaccgga ctgtttattc
    22201 tgtttgccct gaccgtggtg atttttatgg cgaagaaaat atggcttgag cgccagaaga
    22261 ggaatgcctg atgggtttac cgccgcttag caaaattcct ttcattttac gtccgcaggc
    22321 gtggctgcat cgtcgccatt acggcgaggt gctaagcccg attcgctggt gggggcggat
    22381 cccgtttatc ttttatttgg tgtcaatgtt tgttgggtgg ctggagcgca aacgctcacc
    22441 gctcgatccg gtagtgcgat cgcttgtcag cgcgcgcatt gcgcaaatgt gcctgtgtga
    22501 gttttgcgtg gatatcacca gtatgaaagt cgccgagcgt accggcagca ccgataaact
    22561 gctggcagtg gctgactggc ggcaaagccc gctcttcagc gatgaagaac ggctggcgct
    22621 ggagtacgcc gaagccgcaa gcgtaacgcc gccaacggtc gatgatgccc tgcgcacccg
    22681 actggctgcg cattttgacg ctcaggcgct caccgaactg acggcattga tcggcctgca
    22741 aaacctgtcg gctcgtttta attctgccat ggacattccc gctcaggggc tgtgccgtat
    22801 tccggaaaaa cgttcttaag gagagaagat gcgccattgt gggtggttgc tgggattgtt
    22861 atcgctgttt tctctggcaa cacatgccag tgactggcag gaaattaaaa atgaggccaa
    22921 agggcaaacc gtctggttta acgcctgggg cggcgatacc gcaattaacc gctatctcga
    22981 ctgggttagc ggcgagatga aaacccatta cgctataaac ctgaagattg ttcgtctggc
    23041 ggatgccgca gacgcggtga agcgcattca gaccgaagct gctgccggac gtaaaacggg
    23101 cggctcggtg gatctgctct gggtgaacgg cgaaaacttc cgcaccttaa aagaggccaa
    23161 tttattacaa acgggctggg cggagactct gcccaactgg cgctatgtcg acacacagct
    23221 gccggtgcgg gaagattttt cagtgcctac agaaggggct gaatcgccct ggggcggcgc
    23281 acaactgacg tttatcgccc gccgcgatgt tacgccacag ccaccacaaa cgccgcaagc
    23341 cttactggag tttgctaaag ccaatcccgg cacggttacc tatccgcgcc caccggactt
    23401 taccggcacg gcgtttcttg aacagttgct gattatgctg acgcccgatc ccgccgcgtt
    23461 aaaagttgcc ccggataaag ccactttcgc ccgcgtcact gcgcccttgt ggcaatatct
    23521 tgacgcgcta catccctatt tatggcgcaa aggaaaggat ttcccgcctt cgcccgcgcg
    23581 gatggatgct ctgctgaaag ccggcacatt gcgcctgtcg ctgaccttta accctgctca
    23641 tgcccagcaa aaaatcgcca gcggcgattt gcctgcaagc agttacagtt ttggttttaa
    23701 ccaggggatg attggcaacg tgcatttcgt caccattcct gctaacgcaa atgccagtgc
    23761 tgcggcgaag gtggttgcca acttcctgct ctcacccaat gcgcaactgc gtaaagcgga
    23821 tcccgctgtc tggggcgatc cttctgttct cgatccgcaa aaactgcctg atgggcagcg
    23881 cgaaacattg caatcaagaa tgccgcaaga tctgccgccg gtactggctg aaccgcacgc
    23941 aggttgggta aatgcgctgg aacaagaatg gctacaccgt tacggtacgc attaatcttt
    24001 ttgctgtggg cgatggtggc ggtgatttat gcaccactga tcccggcagc tctcacgctg
    24061 atatcgcctg ctttgtcgtt gacacactgg caggcgttat ttgccgatcc acagttaccg
    24121 caggcattac tggcaacgct ggtgtcgaca accatcgcgg cggtcggggc gttgttgatt
    24181 gccctgctgg tgattgtggc gctgtggcct tggccgaaat ggcagcgtat gtgtgcccgt
    24241 ctgccgtggc tgctcgccat tccccatgtg gcttttgcca ccagcgccct tctgctcttt
    24301 gctgacggag ggctgcttta tgactatttc ccgtatttca ctccgctaat ggacaaattg
    24361 ggtattgggc tgggcctcac ccttgcagtg aaagaaagcg catttctgct gtggatctta
    24421 gcggcagtat tgagcgaaaa acggctgttg cagcaggtca ttgtgctgga ttcgctgggc
    24481 tacagccgct ggcaatgcct gaactggctg ctcttgccct ccgtcgcacc tgcactggca
    24541 atggcgatgt tggcgattgt tgcctggtcg ctttcggtcg tggatgtggc gattattctt
    24601 gggccaggta atccaccgac gctggcagta attagctggc agtggttaac tcagggcgac
    24661 gcggatcaac aaacgaaagg cgcactcgcc agtctgctgc tgatgctgtt actcgccgcc
    24721 tacgttttgc tgagctatct gctatggcgc agctggcggc gcactattcc ccgcgtagat
    24781 ggcattcgca agcctgccac acctttatta ccaggcacta cgctggcgag ttttttaccc
    24841 ttaaccggtg tgctgtgtgt ggttctgctg gcgatcctcg cggatcagtc gacgatcaat
    24901 agtgaagcac tcatcaacag cctgacaatg gggctggtgg cgacattcat cgctttgctc
    24961 ctgttactgc tgtggctgga atgggggcca cagcgtcgcc agttgtggct ctggttgccc
    25021 actctattgc ccgctctgcc actggtggcg ggccagtaca cgctggcgct atggctgaac
    25081 ctggatggaa gctggacggc ggtggtctgg gggcatctgc tgtgggtaat gccgtggatg
    25141 ctgtttatcc tgcaacccgc ctggcagcgc attgattcac ggttaatttt gattgcgcaa
    25201 acactgagct ggtcgcgggc caaaatcttc ttttacgtga aatgtccgct tatgctacgc
    25261 cctacgctga ttgccttcgc ggtgggattt tcagtcagta ttgcgcagta tatgccgacg
    25321 ctgtggctgg gcgcggggcg ttttccgacg ctcaccactg aggcggtggc attaagcagc
    25381 ggcggcagca atggtatttt cgccgcccag gctttatggc aactgctatt accgcttatt
    25441 atttttgccc tgaccgcgtt agtcgcaaaa tgggtaggtt atgtcagaca aggactccgc
    25501 taatgctctg cgtgaaaaat gtttcgctac gtttaccaga aagccgcttg ctgacaaacg
    25561 ttaactttac ggtggataaa ggtgacattg tcacgttaat ggggccgtct ggctgtggaa
    25621 aatccactct gttttcatgg atgattggtg cactggctgg acagttttct tgtacaggtg
    25681 agctatggct caatgagcaa cggattgaca tgctacccac cgcacagcgt cagattggca
    25741 ttcttttcca ggatgcactg ttatttgacc agttcagcgt cgggcaaaat ttactgctgg
    25801 cgctaccgtc gacgctcaag gggactgcca ggcgcaatgc cgtgaaagac gcgcttgatc
    25861 gagcgggact ggctgaaact tatcaccagg accctgcgac attatcaggc ggtcagcgag
    25921 cgcgggtggc cctgctgcgc gcccttctcg ctcagccaaa agcgttactc ctggatgagc
    25981 cattcagccg tcttgatgtg gctctgcgcg ataattttcg ccagtgggtg ttcagcgaag
    26041 ttcgcgaact ggcgatcccc gtcgttcagg taacgcacga tctccaggat gttcctgctg
    26101 atagttctgt tctggatatg gagcagtggt cagaaaatta caacaaactg cgataacgca
    26161 aagtttttct caatgcgtca gttcagaatg gcgcactcaa aactacaatg tcgggatttt
    26221 cgatgaaacg tgtttctcaa atgaccgcgc tggcaatggc tttagggctg gcttgcgctt
    26281 cttcgtgggc cgctgaactg gcgaagcctc ttacacttga ccagcttcaa caacaaaatg
    26341 gcaaagcgat agatactcgc cccagcgcgt tttataacgg ctggccacaa accttaaatg
    26401 gcccttctgg tcatgaacct gccgccttaa acctctctgc cagctggctc gacaaaatga
    26461 gcaccgaaca gctcaacgcg tggatcaagc aacataacct gaaagccgat gccccggtgg
    26521 cgctgtacgg taatgacaaa gatgtcgacg ccgtcaaaac gcgactgcaa aaagcaggtt
    26581 ttacgcatat ctccattctg agtgacgcgc taagcgaacc ttcccgtcta caaaaactgc
    26641 cgcactttga gcagctggtt tatccgcaat ggctgcacga cctgcaacaa ggtaaagagg
    26701 ttacggcgaa acctgccggt gactggaaag tcattgaagc ggcctggggc gctcctaagc
    26761 tttaccttat cagccatatt cccggtgctg actacatcga taccaacgaa gtggaaagcg
    26821 aaccgctgtg gaacaaagtt tctgatgaac aactaaaagc gatgctggca aaacacggca
    26881 ttcgccatga caccacggtc attctgtacg ggcgtgacgt atacgctgga gcgcgtgtgg
    26941 cgcaaattat gctctacgca ggcgtgaaag atgtgcgtct gctggatggc ggctggcaaa
    27001 cctggtccga cgcgggactg cctgttgagc gaggaacgcc accgaaagtg aaagcggaac
    27061 cggatttcgg cgtgaagatc ccggcacaac cgcagttgat gctcgatatg gaacaggccc
    27121 gtggactgct gcatcgccag gatgcatcgc tggtgagcat tcgttcgtgg ccagaattta
    27181 tcggtacgac cagcggttac agctatatta aaccaaaagg tgaaatagcc ggagcacgtt
    27241 ggggacacgc tggtagcgac tcgacgcata tggaagattt tcataacccg gatggcacca
    27301 tgcgcagcgc cgatgatatt accgctatgt ggaaagcgtg gaatatcaaa ccagagcagc
    27361 aagtttcatt ctactgcggc accggctggc gcgcgtccga aacctttatg tacgcacgcg
    27421 ccatgggttg gaagaatgtt tccgtgtatg acggcggctg gtacgaatgg agcagcgatc
    27481 caaaaaatcc ggtagcaacc ggtgaacgcg gcccggacag tagcaaataa cattgcaatt
    27541 actgacgctg gtgcgacttc agcgtcagat acccactcca cacccgcgta aaggtcgtca
    27601 tccagcacaa cgcaccgaag atccacgcaa accacgcaaa ccatgcaggg aataaacatc
    27661 ccagcacaaa cagtaagatc gtttctgtgc cttcggttaa cccgcccaga taataaaacg
    27721 atttatgcgc ataaccggga ttatcaatct gatgtttcgc cgccagcgca gcaaaagcga
    27781 gaaaactgct gcctgtgccg ataaacgcaa acagcagcca gccgcctgcc agggcgtttt
    27841 gctgcggtgc ggcgaggata aacccaaaag gtaccagcgc gtaaaaaaga aaatcgagag
    27901 aaatatcaag aaagccgccc gcgtcggtaa gccctctgcg ccgcgccagc gcaccgtcca
    27961 gaccatcaag cagcctgttc aacaaaatga cgacaagcgc cgccagatac cagcccagcg
    28021 ccagaaaagg cagtgccagc acgccgatgg caaatcccac taatgttaaa ccatctggcg
    28081 taatgcccgg tttatcaaga acccgcacgc actgatgcaa caacggttta atccggggat
    28141 gaagatggcg gtctagcaca ggcactcctt aaatataaag cctttctgat tgagcaacag
    28201 tgcggatatt atggcatttt tcgcttatct gcccgtgtgt aatttatgaa aatgattgaa
    28261 gttgttgccg ccatcattga acgtgatggc aaaattttac tcgcgcaacg ccccgcccag
    28321 agcgatcagg cgggattatg ggagtttgcc ggtggtaaag tcgagccgga tgaaagccag
    28381 cggcaggcgc tggtgcgtga gttacgcgaa gaactgggca tcgaagcaac tgtgggtgag
    28441 tatgttacca gccatcagcg agaagtttcg ggccggatta tccatcttca tgcctggcac
    28501 gtacccgact tccacgggac gttacaggca catgaacatc aggcgctggt ctggtgctca
    28561 cctgaagagg cgctgcaata tccgctggcc cctgctgaca ttccattatt agaggcgttt
    28621 atggctttac gcgccgccag accagcggat tagtgctaag ggttttgtca tcccgctggc
    28681 attgcagcag tattccctcg gctttaatta ccgcgccttc agaataattt ttatcctgat
    28741 aaacgcagca ctgagtacag ggctgcgctg actgcccgcc tgaactgaac acttctggcg
    28801 gtacgtttac ctccacgtcc ggacgataat gcgggttagc cagtgcaatt aatggaaatg
    28861 ctaatactac ggcgaacaat gctcgactca cagggaactc cttaacgtta ttgtctctgc
    28921 tactgataac ggtagccggg tggcaaaact ttagcgtctg agttatcgca tttggttatg
    28981 agattactct cgttattaat ttgctttcct gggtcatttt tttcttgctt accgtcacat
    29041 tcttgatggt atagtcgaaa actgcaaaag cacatcacat aaacaacata agcacaatcg
    29101 tattaatata taagggtttt atatctatgg atcagacata ttctctggag tcattcctca
    29161 accatgtcca aaagcgcgac ccgaatcaaa ccgagttcgc gcaagccgtt cgtgaagtaa
    29221 tgaccacact ctggcctttt cttgaacaaa atccaaaata tcgccagatg tcattactgg
    29281 agcgtctggt tgaaccggag cgcgtgatcc agtttcgcgt ggtatgggtt gatgatcgca
    29341 atcaggtaca ggtcaaccgt gcatggcgtg tgcagttcag ctctgccatc ggcccgtaca
    29401 aaggcggtat gcgcttccat ccgtcagtta acctctccat tcttaaattc ctcggcttcg
    29461 aacaaacctt caaaaatgcc ctgactaccc tgccgatggg cggtggtaaa ggcggcagcg
    29521 atttcgatcc gaaaggaaaa agcgaaggtg aagtgatgcg tttttgccag gcgctgatga
    29581 ctgaactgta tcgccacctg ggcgcggata ccgacgttcc ggcaggtgat atcggggttg
    29641 gtggtcgtga agtcggcttt atggcgggga tgatgaaaaa gctctccaac aataccgcct
    29701 gcgtcttcac cggtaagggc ctttcatttg gcggcagtct tattcgcccg gaagctaccg
    29761 gctacggtct ggtttatttc acagaagcaa tgctaaaacg ccacggtatg ggttttgaag
    29821 ggatgcgcgt ttccgtttct ggctccggca acgtcgccca gtacgctatc gaaaaagcga
    29881 tggaatttgg tgctcgtgtg atcactgcgt cagactccag cggcactgta gttgatgaaa
    29941 gcggattcac gaaagagaaa ctggcacgtc ttatcgaaat caaagccagc cgcgatggtc
    30001 gagtggcaga ttatgccaaa gaatttggtc tggtctatct cgaacgccaa cagccgtggt
    30061 ctgtaccggt tgatatcgcc ctgccttgcg ccacccagaa cgaactggat gttgacgccg
    30121 cgcatcagct tatcgccaat ggcgttaaag ccgtcgccga aggggcaaat atgccgacca
    30181 ccatcgaagc gactgaactg ttccagcagg caggcgtact gtttgcaccg ggtaaagcgg
    30241 ctaatgctgg cggtgtagca acgtcaggcc ttgaaatggc gcaaaacgct gcgcgcctgg
    30301 gctggaaagc cgagaaagtt gacgcacgtt tgcatcacat catgctggat atccaccatg
    30361 cctgtgttga gcatggtggt gaaggtgagc aaaccaacta cgtgcagggc gcgaacattg
    30421 ccggttttgt gaaggttgcc gatgcgatgc tggcgcaggg tgtgatttaa gttgtaaatg
    30481 cctgatggcg ctacgcttat caggcctaca aatgggcaca attcattgca gttacgctct
    30541 aatgtaggcc gggcaagcgc agcgcccccg gcaaaatttc aggcgtttat gagtatttaa
    30601 cggatgatgc tccccacgga acatttctta tgggccaacg gcatttctta ctgtagtgct
    30661 cccaaaactg cttgtcgtaa cgataacacg cttcaagttc agcatccgtt aactttctgc
    30721 gatagcagca gatatgccag taaagaaacc ccattagact atttttttga taatcttctt
    30781 cgctttcgaa caactcgtgc gcctttcgag aagcaagcat tatataatgc caggccagtt
    30841 cttcttcaat tgtcccgttt tgaaaagctg tgcttgatat cgaggtcatc catgataatt
    30901 ccgccgccca tattagcttc gccgaggatt taccggagcg attattagcg caatcagaga
    30961 tatagtctga gggaaaaaca gcaaatttat tcaacaaggc gataacctgc tctggggctt
    31021 cctccatgtt tgctttaaag gtattggctc catggtcgcc agaaagaaaa tactccatta
    31081 aggcacaata actttcgcta tcttcgatac cccattgatc ctctaaagac tcgcgtcttt
    31141 tacttatgat atcgatcgag tcaaagggaa gcacatgata ttggaaggta tctttgccag
    31201 gttcaggctt tcgcggccag aactccagcg tttcagacca ttgcttatga tagaaccgat
    31261 aaggtgcgat caattgtagc gcctgtaact tctcgatact gagcggctca atacctttag
    31321 cctgataata atgcagttgt tctttttttg ctttaaaacc ggcccgaaca ataagcccca
    31381 tcataattaa tagataaaga aaagagcatc ccgcggtaat caggcctctt tcattcaaac
    31441 cgttggatgt tatcgctgcg aacacaaaca ttacagcgac aacacatgtt aaataaaa
//
LOCUS       DAZFJF010000046        31311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFJF010000046 DAZFJF010000000
VERSION     DAZFJF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.046"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKAU_002311"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKAU_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO5531370.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHKAU_002312"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHKAU_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO5531371.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTNGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHKAU_002313"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHKAU_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO5531372.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKAU_002314"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKAU_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO5531373.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKAU_002315"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKAU_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO5531374.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKAU_002316"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKAU_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO5531375.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKAU_002317"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKAU_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO5531376.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKAU_002318"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKAU_002318"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO5531377.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKAU_002319"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKAU_002319"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO5531378.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAKRLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKAU_002320"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKAU_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO5531379.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKAU_002321"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKAU_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO5531380.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKAU_002322"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKAU_002322"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO5531381.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     ALRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKAU_002323"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKAU_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO5531382.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKAU_002324"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKAU_002324"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO5531383.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKAU_002325"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKAU_002325"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO5531384.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKAU_002326"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKAU_002326"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO5531385.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKAU_002327"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKAU_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO5531386.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKAU_002328"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKAU_002328"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO5531387.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKAU_002329"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKAU_002329"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO5531388.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKAU_002330"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKAU_002330"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO5531389.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKAU_002331"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKAU_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO5531390.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKAU_002332"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKAU_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO5531391.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKAU_002333"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKAU_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO5531392.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKAU_002334"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKAU_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO5531393.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKAU_002335"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKAU_002335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO5531394.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKAU_002336"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKAU_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO5531395.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKAU_002337"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKAU_002337"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO5531396.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKAU_002338"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKAU_002338"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO5531397.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            31230..>31311
                     /locus_tag="ACHKAU_002339"
     rRNA            31230..>31311
                     /locus_tag="ACHKAU_002339"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7406 a         8108 c         8552 g         7245 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa acggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc aaaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaagtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga ttccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgttctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa gtgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 tgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgcagagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgctatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcaggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagctg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21541 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag cgatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggat atcgccgatg cgcgccgccg
    21661 cgtgggcaat aaagtcgcgt tgcagggcaa tatggatccg tcgatgctgt acgcgccgcc
    21721 agcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agtggcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc cccgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctgc tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatcccg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctagtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagctg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aggccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg agcgggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 agagatggac ggcattgcca cgctaaaaga gatcaaagcg ttaaatccgg cgattccggt
    26641 actgattatg accgcatact ccagcgtcga gacggcggta gaggcactga aaaccggggc
    26701 gctggattat ctcatcaagc cgctggattt cgacaccctg caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aacgcctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgtggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aggatatccc
    27361 tctgctggct agccattttc ttcagcgttt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa acattcgtga
    27481 actggaaaac gcggtggaaa ggtcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg gggcaaagtc aggatattca
    27601 gccgctggtg gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgtgcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gcctgccact tcttccagcg gcaggccgtg
    27961 gatcacgtca ccggtgcggt aatcacccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact ctgacgtttt ctcgtccagt ttgccttcac aggccgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcagcacat gctcgccgtc taccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagt gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaataa ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatctagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttattttttc gcccacattg
    30721 taacgaaaac gtttgcgcaa cgctcgcgaa tttttctctt tcaatggtga tcacaatttt
    30781 gacagtggtt accgtgggaa aaatacagaa attacattga tgattgtgga taactctgtg
    30841 cgtaaaaagg tataaagcgg ggttttgctg gggaatgcag cagtcagtca tttttctgca
    30901 atttttctat tgcggcctgc ggagaactcc ctataatgcg cctccatcga cacggcggat
    30961 gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaatcctga aattcagggt
    31021 tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgcgct aaagcgcgtc
    31081 gcaactgctc tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct
    31141 taacgtcgca agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg
    31201 aagtttaatt ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg
    31261 aacgctggcg gcaggcctaa cacatgcaag tcgaacggta acagaaagca g
//
LOCUS       DAZFJF010000047        31011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFJF010000047 DAZFJF010000000
VERSION     DAZFJF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.047"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(314..1904)
                     /gene="lsrK"
                     /locus_tag="ACHKAU_002340"
                     /pseudo
     CDS             complement(314..1904)
                     /gene="lsrK"
                     /locus_tag="ACHKAU_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
     gene            complement(1983..2936)
                     /gene="lsrR"
                     /locus_tag="ACHKAU_002341"
     CDS             complement(1983..2936)
                     /gene="lsrR"
                     /locus_tag="ACHKAU_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO5531398.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3185..4720
                     /gene="lsrA"
                     /locus_tag="ACHKAU_002342"
     CDS             3185..4720
                     /gene="lsrA"
                     /locus_tag="ACHKAU_002342"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO5531399.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4714..5742
                     /gene="lsrC"
                     /locus_tag="ACHKAU_002343"
     CDS             4714..5742
                     /gene="lsrC"
                     /locus_tag="ACHKAU_002343"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO5531400.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5742..6734
                     /gene="lsrD"
                     /locus_tag="ACHKAU_002344"
     CDS             5742..6734
                     /gene="lsrD"
                     /locus_tag="ACHKAU_002344"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO5531401.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6746..7768
                     /gene="lsrB"
                     /locus_tag="ACHKAU_002345"
     CDS             6746..7768
                     /gene="lsrB"
                     /locus_tag="ACHKAU_002345"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO5531402.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7795..8631
                     /gene="lsrF"
                     /locus_tag="ACHKAU_002346"
                     /pseudo
     CDS             7795..8631
                     /gene="lsrF"
                     /locus_tag="ACHKAU_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            8630..9124
                     /locus_tag="ACHKAU_002347"
                     /pseudo
     CDS             8630..9124
                     /locus_tag="ACHKAU_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(9128..10042)
                     /gene="yneE"
                     /locus_tag="ACHKAU_002348"
     CDS             complement(9128..10042)
                     /gene="yneE"
                     /locus_tag="ACHKAU_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO5531403.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10249..11700)
                     /gene="uxaB"
                     /locus_tag="ACHKAU_002349"
     CDS             complement(10249..11700)
                     /gene="uxaB"
                     /locus_tag="ACHKAU_002349"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO5531404.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(11927..13345)
                     /locus_tag="ACHKAU_002350"
     CDS             complement(11927..13345)
                     /locus_tag="ACHKAU_002350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5531405.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAIWPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13484..13843)
                     /gene="yneG"
                     /locus_tag="ACHKAU_002351"
     CDS             complement(13484..13843)
                     /gene="yneG"
                     /locus_tag="ACHKAU_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO5531406.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13843..14769)
                     /gene="glsB"
                     /locus_tag="ACHKAU_002352"
     CDS             complement(13843..14769)
                     /gene="glsB"
                     /locus_tag="ACHKAU_002352"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO5531407.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14833..16221)
                     /gene="sad"
                     /locus_tag="ACHKAU_002353"
     CDS             complement(14833..16221)
                     /gene="sad"
                     /locus_tag="ACHKAU_002353"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO5531408.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16322..17203
                     /gene="yneJ"
                     /locus_tag="ACHKAU_002354"
     CDS             16322..17203
                     /gene="yneJ"
                     /locus_tag="ACHKAU_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5531409.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17281..18395
                     /gene="yneK"
                     /locus_tag="ACHKAU_002355"
                     /pseudo
     CDS             17281..18395
                     /gene="yneK"
                     /locus_tag="ACHKAU_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            18545..19735
                     /gene="ydeA"
                     /locus_tag="ACHKAU_002356"
     CDS             18545..19735
                     /gene="ydeA"
                     /locus_tag="ACHKAU_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO5531410.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(19760..20425)
                     /gene="marC"
                     /locus_tag="ACHKAU_002357"
     CDS             complement(19760..20425)
                     /gene="marC"
                     /locus_tag="ACHKAU_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO5531411.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            20637..21071
                     /gene="marR"
                     /locus_tag="ACHKAU_002358"
     CDS             20637..21071
                     /gene="marR"
                     /locus_tag="ACHKAU_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO5531412.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21091..21474
                     /gene="marA"
                     /locus_tag="ACHKAU_002359"
     CDS             21091..21474
                     /gene="marA"
                     /locus_tag="ACHKAU_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO5531413.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21506..21724
                     /gene="marB"
                     /locus_tag="ACHKAU_002360"
     CDS             21506..21724
                     /gene="marB"
                     /locus_tag="ACHKAU_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO5531414.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(21781..23220)
                     /locus_tag="ACHKAU_002361"
     CDS             complement(21781..23220)
                     /locus_tag="ACHKAU_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5531415.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23245..24918)
                     /locus_tag="ACHKAU_002362"
     CDS             complement(23245..24918)
                     /locus_tag="ACHKAU_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5531416.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(24974..25285)
                     /locus_tag="ACHKAU_002363"
     CDS             complement(24974..25285)
                     /locus_tag="ACHKAU_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO5531417.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25313..26635)
                     /locus_tag="ACHKAU_002364"
     CDS             complement(25313..26635)
                     /locus_tag="ACHKAU_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO5531418.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFDLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(26750..27061)
                     /locus_tag="ACHKAU_002365"
     CDS             complement(26750..27061)
                     /locus_tag="ACHKAU_002365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5531419.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27260..27958
                     /locus_tag="ACHKAU_002366"
     CDS             27260..27958
                     /locus_tag="ACHKAU_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5531420.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(28003..28902)
                     /gene="eamA"
                     /locus_tag="ACHKAU_002367"
     CDS             complement(28003..28902)
                     /gene="eamA"
                     /locus_tag="ACHKAU_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO5531421.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            29097..30284
                     /gene="ydeE"
                     /locus_tag="ACHKAU_002368"
     CDS             29097..30284
                     /gene="ydeE"
                     /locus_tag="ACHKAU_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO5531422.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            30411..30506
                     /gene="mgtS"
                     /locus_tag="ACHKAU_002369"
     CDS             30411..30506
                     /gene="mgtS"
                     /locus_tag="ACHKAU_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO5531423.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            30506..30610
                     /gene="mgtT"
                     /locus_tag="ACHKAU_002370"
     CDS             30506..30610
                     /gene="mgtT"
                     /locus_tag="ACHKAU_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO5531424.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(30725..>31011)
                     /gene="dgcZ"
                     /locus_tag="ACHKAU_002371"
     CDS             complement(30725..>31011)
                     /gene="dgcZ"
                     /locus_tag="ACHKAU_002371"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO5531425.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         8099 a         7683 c         7199 g         8030 t
ORIGIN      
        1 taagaaaact gtttctttca ataggaaaat tatcatcatc atatttaaca aagaatagca
       61 ctaattgcta aaaatcgaag tttattaaac cccttttgca tccattaatg tctttaggaa
      121 tattcgctat aaaataaggg ttatgcttaa tgctttaaga aaaatagcaa ttttcccttg
      181 aatatagtac tggtgatgga acgatgaatc tgcaagctgg catttaacaa gccagctcta
      241 aaagaaggga aataagaaaa actatactca aaaactaaca gccacggtca tcatgatgtg
      301 gccgtcaatg aaactataac ccaggcgctt tccataacga cgtcgtcagt ccatgatcaa
      361 ccagccccag ctgatcctga taaactgcct gccacttatc gcgtgaatcc tgataaagtt
      421 catgcttttc cgggtctggt gtgtgcgtcc gttcccagcg aaccaggcgt tctccggttt
      481 ctgccattga tgaaaaaatt ccggcaccga cgccagctgc aatggcacat cctaatgcgg
      541 tggcttcttt gactaccgga atattgacgg gtaatcccga gacatcagcg agaatttgac
      601 tccataattt cccttttgaa cctccgccag caaaaaccag cgaggtggga tgaatattcg
      661 agaaatcagc aatttgctgc aagttacacg ctgatacaat cgccgcattt tcttccagcg
      721 cacggaacaa tgtcgctttg ttacatttat ccgggtcaat ggacaagtta ataaaggaag
      781 gcgcagcgtg ataccaggtt ttaaagcgca ttctgtcgga gaagatcggc attacgcccc
      841 acgacccagg cggcacccga ctggccatct cttccagcag cgtataggtg tcgatgccta
      901 aacgttcggc aatcagtttt tcttcggcac agaaagcatc gcggaaccag cgcatggtga
      961 gtccggtaaa aaagcttata gattcagctt gtaccatgcc aggaataaca tgaggattaa
     1021 cgcgcacgtt catttctggg tctgtcaccg gcgcggctaa atttacaact tgctgccaga
     1081 atgtgccgcc aagaaccgcg gtttgtgccg gacgcacaac gcctaaccca aggcaaccaa
     1141 gctgcacgtc gcctcctcca acgaccaccg gagtgcccgc cttcagaccg cagagttccg
     1201 ccgcttgtga acttaccacg cccagcaatg tgccggtttc tttgacagga gaaagaatat
     1261 cggcacgcag gccagccata tccagcaatg caggtttcca gtcacgggtg gttagatcaa
     1321 agtcccgtgg tgccagcgtt agagggatcc accgccagtt cgccgctgag catataggcc
     1381 agccagtcgc tgatcatggt gatggttgat gcctgacggt aaatatcgga acgatggtgc
     1441 gccagccaaa gtaatctggg gatggcactt aaagccagtg tttgtccggt cgcgcgataa
     1501 acttcgtttt caaaggtatt gttgtgcagt tctttaagtt cgctaacttc gcgcgctgct
     1561 ctggcatcca cattggcgca ggcccagatc ggtgttcctt cattgttata taaaacaatg
     1621 ccttcacgca tagaacatgc cgaaacggca gcgatatact ccgggtctat gccggcgttg
     1681 tgcagcgcct ggcgcataca ctcacacgcc agttgccagt ttttgttgag atcaaattcc
     1741 atagaaccag gaacgtccgg tactgccaga tgccgccact ccgcctgtcc cactgctatt
     1801 tgattgcctt tcaggtcgaa aatcacagcc cgaatacttc cggtgcctgc atccagcgcc
     1861 aacaggtaat actttgattc tgaaggggta aagagtcgag ccattagatt atcctcggct
     1921 tatgcgttct gtctgttcct ctatacgttc tccatcattc ccggtaataa ggtcatgcaa
     1981 atttaactac gtaaaatcgc cgctgctgta tcctgatcgg taaccagtgc gttgatataa
     2041 ccgcctttca ttgcagcggc aattgcttcg gctttatttt ctccccctgc cacgccaacc
     2101 cggacgggta tggtcttcag cgcgcttaaa ggtaagccaa tcagttcgtt atgtatttta
     2161 atatccgtga caacgtcacc ttttgcatca aaaaagtagc ctaaaatgtc gccaaccgcc
     2221 ccttttcgac caatcattaa ctgttcgccc tggctgatat aaccggagcg aatgatcgtc
     2281 gcgtcgtcct gttgactgac ggccccaatg ccgacaatcg ccacatccgc tgcttgcgcg
     2341 gctaacagaa catctttgac gcaattttca ttttttagcg tacgggcaat gtcagcggag
     2401 gatgcccgca acggagccgg aataatattc acactgcacg ccgcgttaag ctgcccgata
     2461 cccgtcatat aagaaccgac gccaccggag agcgtgacca ggcgaatttg ctgtgacgaa
     2521 ataaaaccac ttaagcgttg cagcgtattc atggttgcct cgccaaaacc aatcgccagc
     2581 atctgttgtg gttgaagtaa actcatcaac atatgcgccg cgcctatccc tagtcgcccg
     2641 ccgacatcag catccgcaag cccagggatc acccggacat gttgcagcga aaactgacga
     2701 cgtaattgag tttcatattc cagacagcct tcaaagcgag aattaatctg tacgcgaata
     2761 atgccggact gatgcccttt ctccagcaag cgcgacactt tcaaacgggt caggccgaga
     2821 cgatcgctga tctcgctctg ggtcagcccg tcgtgatagt aaaaccacgc gatccgcgcg
     2881 acctgttctt cttcacacat tccctgttct gaaattgccg aatcgttgat tgtcataatt
     2941 cattcttcac tttgaacata tttaaatctt taatgcaatt gttcagttct tgcgcattta
     3001 tatctgtgat ggcagccaca gtttgactct acgagcatga acaaacgcaa ccgtgaaaat
     3061 caaaatagca taaattgtga tctattcgtc ggaaatatgt gcaatgtcca cctaaggtta
     3121 tgaacaaatt aaaagcagaa atacatttgt tcaaaactca cctgcaaaac tgaacggggg
     3181 aaatatgcaa acgagtgata cccgcgcgtt accgctactt tgcgcccgct cggtttataa
     3241 acagtattca ggggtcaatg tcctgaaagg catcgatttt acgttgcatc agggggaggt
     3301 ccacgccctg ctcggcggca atggtgccgg taaatcgacg ttaatgaaga ttattgccgg
     3361 tattacccct gctgatagcg gtacgctgga gattgggggc aacaactacg ccagattaac
     3421 gccagttcat gctcatcagc tgggcattta tctcgttccc caggaaccgc tgcttttccc
     3481 aagcctgtcg ataaaagaaa acatcctgtt tgggatggca aaaaaacagc tctccatgca
     3541 aaaaatgaag aacttgctgg cggcgctggg ctgccagttt gatctgcata gtctggcagg
     3601 atcgctggat gtcgccgatc gccaaatggt ggaaatcctc cgcgggctaa tgcgcgactc
     3661 gcggattctg atcctcgatg aacctaccgc ctcgcttacc cctgcggaaa ccgaacgctt
     3721 gtttactcgc ttgcaagagc tgcttgctac tggcgtgggt attgttttta tctcgcataa
     3781 gctgccggaa attcgccaga ttgccgatcg aattagcgtg atgcgcgacg gaaccatcgc
     3841 cttaagcggc aaaaccagcg aactgtctac cgacgacatt attcaggcca tcacgccagt
     3901 ggtacgggaa aaatcgctct ctgccagcca aaaattatgg ctggagttac ctggtaaccg
     3961 cccacaacat gctgccggaa cgccggtgct gacactggaa aatctgaccg gcgaaggttt
     4021 caggaatgtc agcatgacgc tcaatgccgg agaaattctg ggcctggctg ggctggtggg
     4081 ggccggacgc acagaactgg ccgagacgtt ctatggtctg cgtactttgc gtagcggacg
     4141 cattatgctg aatggtaaag agatcaataa attatccact ggagaacgtt tactgcgcgg
     4201 tctggtttat ctgccggaag atcgccagtc atccggactg aatctcgatg cttcgctggc
     4261 ctggaacgtc tgcgccctta ctcataacct tcgtggattc tgggcgaaaa ccgcgaaaga
     4321 taatgccacc ctggaacgtt atcgtcgggc gctgaatatt aaatttaacc aaccggaaca
     4381 agctgcacgg acattatccg gtggcaacca gcaaaaaatc ctcattgcca aatgcctgga
     4441 agcttcgccg caagcattga ttgtcgatga gccgacgcgc ggcgtggatg tctcggcccg
     4501 taatgatatc taccagctgt tgcgcagcat ctccgcacag aatgtggctg tgctgcttat
     4561 ctcctctgat ctggaagaga tcgaactgat gacagatcgt gtgtatgtga tgcatcaggg
     4621 cgaaattacc cactctgcac tgaccgggcg cgatattaat gtcgagacta ttatgcgcgt
     4681 tgccttcggc gatagtcagc gtcaggaggc atcatgctga agtttattca gaacaaccgt
     4741 gaaatcacgg cattgctggc ggtggtgctg ctgtttgtat tacccggttt tctcgaccgt
     4801 cagtatttaa gtgtgcaaac gctgaccatg gtttatagca gcgcgcaaat cctgatcctg
     4861 ctggcaatgg gcgcgacact ggtaatgctt acgcgcaata ttgatgtttc agtgggttcg
     4921 attaccggaa tgtgcgcggt gctgttgggg atgttactga acgcaggata ttcactacct
     4981 gttgcctgtg tcgcgacttt actgcttggt ttgctcgcgg gatttttcaa cggtgtcctg
     5041 gtcgcgtggc taaagatccc tgccattgtt gccacccttg gcacgttagg gttgtacaga
     5101 ggcatcatgt tgctgtggac tggcggtaaa tggattgaag ggttacccgc cgaactgaaa
     5161 cagctctccg caccgctgct gtttggcgtt tccgcgattg gttggttgac gataattctg
     5221 gtggcattta tggcctggct gctggcaaag acggcgtttg gacgcagttt ttatgccacg
     5281 ggcgataatt tacagggcgc tcgtcaactg ggtgttcgta cggaagccat tcgcattgtg
     5341 gcattttcgt tgaacggctg catggcggca ctggcgggaa ttgtgtttgc ttcgcagatt
     5401 ggttttatcc ccaaccagac cggtaccggg ctggagatga aagcaattgc agcctgcgtg
     5461 ctgggcggca ttagtttgct cggcggttcc ggtgcgatca tcggtgcggt actcggcgca
     5521 tggttcctga cgcagatcga tagcgtactg gtgctgttgc gcattccggc atggtggaac
     5581 gattttatcg cgggcctcgt gctgctggcg gtgctggtgt ttgatggacg cctgcgttgt
     5641 gcgctggaac gtaatctacg gcggcaaaaa tatgcccgct ttatgacgcc accgccatcc
     5701 gttaaacccg cttcgtcagg taaaaaacgg gaggccgcat aatgcgtatt cgctacggtt
     5761 gggaactggc tcttgccgca ctgctcgtta ttgagattgt cgcatttggt gcaattaacc
     5821 cgcgaatgtt agatctcaat atgttgctgt tcagcaccag tgactttatc tgcattggca
     5881 ttgtcgccct accgctgacg atggtgattg tcagtggcgg gatcgatatt tcgtttggtt
     5941 cgaccatcgg cctctgcgcc attgcattgg gcgtactgtt tcaaagtggt gtgccgatgc
     6001 cgctggcgat actcctgacc ttactgctcg gcgcattgtg cgggctgatc aacgccggat
     6061 taattatcta taccaaagtt aacccgctgg tgattacgct tggcacgctg tatctgtttg
     6121 ccggaagcgc tctgctgctt tccggtatgg ccggagcgac ggggtacgaa ggtattggtg
     6181 gattcccgat ggcgtttaca gatttcgcta acctggatgt gctgggactc cccgttccgc
     6241 tgattatctt cctgatatgt ctcctcgttt tctggctctg gctgcataaa acccatgccg
     6301 gacgtaatgt gtttttgatt gggcaaagcc cgcgcgtggc gctttatagc gcgattccag
     6361 ttaaccgcac cttatgtgcg ctctatgcca tgacggggct ggcgtctgcg gtcgccgctg
     6421 tgctgctggt atcgtatttt ggttcagcac gttccgatct cggtgcgtcg tttctgatgc
     6481 ccgccatcac cgccgtggtg cttggcgggg ccaatattta tggtggttcc ggttccatta
     6541 tcggcaccgc cattgcggtt ttattagtgg gatatttgca gcaaggtttg caaatggcag
     6601 gagtgccaaa tcaggtgtcc agcgcccttt ccggtgcgct acttatcgtc gttgtcgtag
     6661 gtcgttccgt tagcctgcat cgccagcaaa ttaaagagtg gctggcgcgt cgggccaata
     6721 acccattgcc ataaaggata tcttcatgac acttcatcgc tttaagaaaa tcgccttact
     6781 tagcgctctt ggcattgccg caatctctat gaatgtgcag gccgcagagc gtattgcatt
     6841 tattcccaaa ctggttggcg tgggattttt taccagcggt ggcaacggcg cacaacaagc
     6901 gggtaaagag ctgggcgttg atgtgaccta cgacgggccg acagaaccca gtgtttctgg
     6961 tcaggtacag ttgattaata acttcgtcaa tcaaggttat aacgccatta tcgtttctgc
     7021 ggtttcgcct gatggcttgt gtccggcact gaaacgcgcc atgcaacgtg gtgtgagagt
     7081 gctgacctgg gactctgata ctaaaccgga gtgccgctct tactacatta atcagggaac
     7141 gcccgcccag ttgggaggta tgttggtgga tatggcggcg cgtcaggtga ataaagacaa
     7201 agccaaagtc gcgtttttct actcaagccc caccgttacg gaccaaaacc agtgggtgaa
     7261 agaagcgaaa gcgaaaatcg ccaaagagca tcctggctgg gaaattgtca ctacgcagtt
     7321 tggctataac gatgccacta aatcattaca aaccgcagaa ggaatattaa aagcgtatag
     7381 cgatctcgac gccattatcg cccccgacgc caacgccctg cccgctgccg cacaagccgc
     7441 agaaaacctg aaaaatgaca aagttgcgat tgtcggattc agtacgccaa acgtgatgcg
     7501 tccatatgtg gaacgcggca cggtgaaaga atttggcctg tgggatgtgg ttcagcaagg
     7561 caaaatttca gtgtatgtcg cggatgcatt attgaaaaaa ggatcaatga aaacgggcga
     7621 caagctggat atccagggcg taggtcaggt ggaagtctcg ccaaacagcg ttcagggcta
     7681 tgactacgaa gcggatggta atggcatcgt actgttaccg gagcgcgtga tattcaacaa
     7741 agagaatatc ggcaaatacg atttctgatg tgcattactt aaccggagta agttatggca
     7801 gatttagacg atattaaaga tggtaaagat tttcgtaccg atcaatcgca acaaaatatc
     7861 ccttttatcc tgaaaggttg cggtgcgctg gattggggaa tgcagtcacg cttatcgcgg
     7921 atatttaatc cgaaaacggg taaaaccgtg atgctggcct ttgaccatgg ttattttcag
     7981 gggccgacta ccggacttga acgcattgat ataaatatcg ccccgctgtt tgaacatgcc
     8041 gatgtattaa tgtgtacgcg cggcattttg cgcagcgtag ttcccccagc gaccaataag
     8101 ccggtggtac tgcgggcgtc aggagcgaac tctattctgg cggaattaag taatgaagcc
     8161 gtggcgttat cgatggatga tgccgtgcgc ctgaacagtt gcgcggtggc ggcgcaggtt
     8221 tatatcggca gcgaatatga acatcagtcg atcaaaaata ttattcagct ggttgatgcc
     8281 ggaatgaaag tgggaatgcc gaccatggcc gtgactggcg tgggcaaaga tatggtgcgc
     8341 gatcagcgtt atttctcgct cgcgactcga atcgccgctg aaatgggggc gcaaattatc
     8401 aaaacctatt atgtcgaaaa aggttttgaa cggattgttg ccggatgtcc ggtacccatt
     8461 gttattgctg gcggtaaaaa attaccggag cgcgaggcgc tggaaatgtg ctggcaggct
     8521 atcgatcagg gcgcttctgg tgtggatatg gggcgtaata ttttccagtc tgaccatccg
     8581 gtggcgatga tgaaagccgt acaggcggtg gttcaccata acgaaacggc aaccggaaca
     8641 ggcactcgat ctgatttttg ctaatgcctc actgcaatgg ctgcccgacc actacgaatt
     8701 gtttcctcat ctggtttctt tacttaatcc gcagggcgtg ctggcagtac agatgccaga
     8761 taactggctg gagccgaccc atgtgctcat gcgcgaagtt gcctgggaac aaaactaccc
     8821 ggatcgcggg cgtgagccgt tggctggcgt tcatgcttac tacgatattt tgagcgaagc
     8881 cggatgtgag gtcgatatct ggcgaaccac ttactatcac cagatgccgt cgcaccaggc
     8941 gattatcgat tgggtgaccg ccactggatt acgtccgtgg ttacaggatc tgaccgagag
     9001 cgaacagcag ctttttctta cgcgctacca tcagatgctg gaagagcagt atccactgca
     9061 agagaacgga cagatactgc tggcatttcc gcgtctgttt attgttgccc gccgtacgga
     9121 gtaattatca cgtcagctgg taatgacgat cgggaagaat ttttgctgga atttctgctt
     9181 catcgttcat ctgtaacagg tcaatttcaa tagcgttgca gatggcatcc agcggtaaat
     9241 cattgttttc agtaccgaac ggatcttcca gttcttccgc cagacaatcc agcgaaataa
     9301 aagtgtagga aatcagcaca gagataaaag gcgtcatgta atgcaggtcc acgaccagcg
     9361 cgaacggcag catgatacaa aacagataaa cggtacgatg caaaatcagg gtgtaggcaa
     9421 agggaattgg cgtgtaggca atgcgctcac agcctgccag aaccgctgaa atatcattaa
     9481 gccgatcgtt gaggctaata aactggatat ctgaaagctg cccattgcgg cgctgaaccg
     9541 ccaaccactc ccccattatt aacaagatac ggttagctgg agagttcgaa gccagtacag
     9601 gctgaagatc ttcagtcttg agataatgag ccagcacttc cgcctgtggc tgtttgcgta
     9661 atgtcatgcg taaacagtgg gcgaaggcga tttgcaaccg ggcaaactcc cttacacttg
     9721 ccgaatccgg caatgtcgtt tttacctcgc gcagtaacga ccgtgaggca atcatcaact
     9781 gcccccaaag ttttcgcgct tcaacgtaac gggcgtaccc ggcattatta cgaaaaccaa
     9841 gaaaaatggc gatggcgaca ccgagaatgc tgaacggtgc gagggtgaat ttgatgccca
     9901 gatgcgtgta ccagggcagc atgaaaataa cagcgataga aaagagaaaa ttgagtagta
     9961 agcgcgatga tatcttggat aatactgagc cgtgccagac aaaaatacgg cgcagccagt
    10021 gttgttgtgg acgaacaatc atggttatct tcaggcgtga aaaagtcgcc ctattaaacg
    10081 tgattacgat cacattctca agacgctact tacaaattac ctacaagctt acaacattaa
    10141 caggcgctgc atgtggcatc tgttaatgat aaaggcacat aggatgttgt aactaatata
    10201 gtgaaataag aaacccggtc gaaaccgggt tcagaagtaa cggtgttatt agcacaacgg
    10261 acgtacagct tcgcgcatcc ctttttcaag aatcgcatcc aggtcattag caacctgctc
    10321 gaccagacca ggcacttgcg tcaggtcctg ctcccagtgg tctttttctg ccaacacaat
    10381 cgctaccagt tcctgggtac cgataacacg gtcacgatgc tggctccaga gttgctgata
    10441 acgttccagc cagtgtgcat cgtcctgtac cggatatgtt tctccgttac gctcaccacg
    10501 atagaaggca atcaatgccg ctaatgcgaa agtaaggcgc gccggaagtg tgccttttgc
    10561 cttctgccgt gccagcagct gcggcaggat gcgagtgcgg aacttggtca taccgttgag
    10621 cgcgatagac agcagctgat gcttaatgta cgggttacgg aaacgcccgg tgactgcact
    10681 ggcgaaagat tccagttcat cacgaggcaa atccagtacc ggaataattt cttcgtagat
    10741 agctttttca acgaatgcgc aaatttcagc atcgttcatc gcttcaccta cggtatccag
    10801 ccctgcctgg aacgccaccg gcaccagcgc ggtgtgcgca ccgttgagga ttgccacttt
    10861 gcgttctttg tacggtttaa tatcgtcaac aatcagcacg ttgagcggat atttgtccag
    10921 acgcagttca gtcgctaagg atttcggtcc ctgaatcaca aacaggtaaa agtgttcagc
    10981 ggtgtcgaga aaaccatcgt gataacccag ctcttcttcc agtttagcca cttcatcgcg
    11041 cggataaccg gtaacgatac ggtccaccag cgtagaacag aagctgttag cctgatccag
    11101 ccattgaata aatgcttctg gcagtgccca ctcttgcgca tagcgcagca ccagttcacg
    11161 caacgcgtcg ccattgtagt cgatcaactc acacgggatg atgatccaac ctttatccag
    11221 cgcaccgttg aaatggctaa aacgttcgaa cagcagacgg gtcagttttg ccggataact
    11281 taccgctggc gcgtcatcga atttatcgcc cgcatggtag ctgatacccg cttcggtggt
    11341 attagagaaa acaaagcgca tttccgggtt gtgagccagt ttcaggaatt catcgtattc
    11401 actgtagacg ctgatttcac gattaaccga gcgaatcaga cgcgcgtcgc tgaccgcttc
    11461 ccccttctca ttcaggccac ggataatggt ggtatacaga ccatcctgcg tgctcagtga
    11521 cggtgggaat gaagtttcaa tcggacgaac aacgaccaca ccagaattca gatcggtgtg
    11581 ctcattcagg agatcgattt gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa
    11641 ctgaatgata cgttctggat actgtgcacc gggaaaatcg cgacgattta gtgttttcac
    11701 aatgggttcc cttctgatta gtcatacaac ctgtttgaat tggtacgaca ggttagcaaa
    11761 ctttaatacg ccgaacccct gttttgatca actcctgatg attaatgagc agttttatga
    11821 gaaaagtgtg gcgtggatca tggtttaatc gaggaaatac gccttttcct ggatcataaa
    11881 gtggtagaac acattgcatt caaatcgcgc ggaatgaata aagatgtcag acaacttcct
    11941 caccgtaacg catagtgctg gtacggttgc gcccatcttt cttcgagcga tacagacacg
    12001 tatcggcttc gaccatcaat ttattaaaat catcggtcag cgttcggtac gatgcgcgac
    12061 cactaccgac gccaatactt accgtgagat aaagcgtttt ttgttgccag gtgaatggtt
    12121 gcagttcaac gcctttacgg attttttccg ccattagcag accatcgaca ggattcaccg
    12181 acggcactgc aacagcaaat tcttcgccgc ccatgcgcgc caccagcccc ttatcaccga
    12241 caatcttctg aatatgccgg gcaaacacgc ttaacacttt atcgccacat tcatgtccat
    12301 agttatcgtt aatgcttttg aaatagtcga tatcaagcag catgaccgtc agatgttgtg
    12361 tctgtttcag cgatggactt ttcagcgcct cataaagacc ggaccgtgag taaacctgag
    12421 tcagaaaatc aaagtcggct cgcagcgcaa cttgcttcat tagcgaattg atcgctgcca
    12481 cgctaaaaga aaccataatt ggccatatcg ccatcgtggc aataccgaga cgtgcggaga
    12541 acatttccgg aatggagaac ggcgaaccga ccgagatatc aatcaccgaa tttgccacca
    12601 gtacgatttc caccgcaccg gtgacaaagg tcaacagaca tgttacctgc ggcgtatagc
    12661 gcactgcaca ccagattaat gcaggcagcg ggaacgccag actccccgct ccgccaatga
    12721 ctaccgaggc aataaccgac acaataagcg cgatggcggg catcatctgt tctgctttaa
    12781 agcgcggcag cactccagga atagccaacg tcagcataca aggcacgatc aacacgcccg
    12841 ttgagaattg ctcactgaac cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac
    12901 tgaccgaacc aatcgccccg acaatagcgc ataataatgc ggcaatcaga cagtaattaa
    12961 atagccgtaa ggcacttact ggctcatact tatttttccc cagacgctta tcccgcacga
    13021 ccagtaaggc gacggtaaca ataaacatca tattggagaa attgataacc agtgaaacca
    13081 gtccccattc ggtggttatg gcatcataaa ccagcatcgc cacataactg atcgcatagt
    13141 aatgcagtcg attaagccag acatagcggg caaacacccc cgccattacg ccattcagcg
    13201 gccagaaaag agaaagttcg tgaaccagcc gtaactctgc accaataaaa tagaacagcg
    13261 tggttagcgc aaaaatagcg atgctattac gtagcagatg accttcctgg aacaaacgga
    13321 aagttgaaat ggggtgtaca tgcattaatt atctgtccat acaattgcgg gtggcaatta
    13381 tctcaacgta ataagtaaat tctcataaag gatagcatgt tcgctaacag cggcggcaaa
    13441 taaaggtacc ctgggaatgc tattttatcg ttttccgcaa ttctcatggc tgattcatct
    13501 gtacaaccag ccagtgatag attaccgcca cgatataacg ttgttgttct tcgcttaacg
    13561 aaacaccctg cggaatgtta tgccacttag ccagacaacc gcgacagcag gtcgcggtgg
    13621 cgtgctgagc gataaacacc ggatgcccgc gcatgggggt ttgcttaccg tcattagccg
    13681 gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc atgttgttcg atgactggcg
    13741 cgcctttctc caggcaatac tgacgctctt tcatgcccag acgaaagcga gagcgaaatt
    13801 ttgaacggga taaacgcgcg aagagtggat cgagagactg cattaataaa ccgaacgccc
    13861 taactgtttc gtcaattgtt caaggacggc aatacccgca agcgagttac ctgcatcatc
    13921 cagttccgga ctccagacag cgatggccat ttcatgcgga acaatcgcca caataccgcc
    13981 accaacgcca gatttcgccg gtagccccac ccgccaggca aactcccccg cgttctggta
    14041 cataccactg gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcaccacagg
    14101 ttcatcaata tgaatagctt tcccctgatt agccagaaag acaaacgtcc tggccagctc
    14161 tacacagctc attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacatc
    14221 atgatggaaa ttgccaaacg acttcatcag ccaggcgata gccgcatttc gcgcggaatg
    14281 ttcaaattcg gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc
    14341 gcgcacgact tccagcatac gttgccgtgg tgcgcttaat cgcccttgca acatatcgca
    14401 aaccaccagc gcaccagcat taataaacgg attacgcgga ataccctgct ccatttccag
    14461 ttgcactaaa gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc
    14521 ctcttcggag taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat
    14581 ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc
    14641 aatgcccaat cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc
    14701 ctgaccaatg agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc
    14761 gactgccact ttctactcct ggaccgcagg tctgaaaaga cctgcgagta tatcagagct
    14821 gaatatgtcg cgtcagatcc ggtctttcca caccgtctgg atattacaga attcgtgtaa
    14881 gccgaaatgg gaaagctcac gaccaaagcc actctttttc acgccaccaa aggccactcg
    14941 cgcgtcgctg gcacaataac cattgataaa caccccaccg cattccagac gtgccgccat
    15001 ctgtctggcc tgtgtttcgt cggtggtaaa aatggtcgct gaaaggccga actcactatc
    15061 attagccagt tccagcgcat gttctgcatc tttcgcaacg gtgattgccg caacagggcc
    15121 aaacatttct tcccgaaacg cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg
    15181 atagtagtta cctgccccag ccatcttttc cccgcccagt aacaaacgcg caccctgcgc
    15241 cagggttttc tccacctgat gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc
    15301 gagagcattc tcttcatcac ggggatcgcc cattttcaag gctgccgcag ctgccacaaa
    15361 acgttcggta aatgcagaag caattccctc ttcgataata aagcgttttg ccgctgcaca
    15421 aacctgtccg gtattctgat aacgtccggc taccgccgct ttaaccgcca gttccagatc
    15481 ggcatcgtta agcacaataa atggatccga accgcccagt tccagtacgc attttttcag
    15541 tgccgctcca gcctgtgcgc caatagccgc tcccgcacga acacttccgg tcaccgtgac
    15601 agcagcaatg cgcgaatctt taatcatttg actgacaccg tcgttgtcgg cattcagcca
    15661 gccatatacg ccttgcggga ttcccgcatc tttaaacacc tgggcaatga gctgagcaca
    15721 gcccatcaca ttcggcgcat gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac
    15781 cgcgccacgc atcacctgcc ataacggaaa gttccacggc ataatcgcca gaatcgtccc
    15841 caacggtcga tactcaataa ctgcctgctg attttccacc agcgtaggtt ccgccttcag
    15901 cattgccgga ccatgttctg cataccagtc acacaaattc gccgatttcg ccacttcagc
    15961 gcgcgcctga ttgattggtt tgcccatttc acgggtgatc atttgcgcca tttcttcgct
    16021 acgggcgcgc agagccttac cgataccacg cagtttttca gcacgataat ctatatttgt
    16081 ctcgcgccag tcgcgaaagc ctgctgccgc cagctgaagt gcgttttcga tatcgttagc
    16141 gccagcccac ggcagcacag aaagttgttc acccgtggca ggatttattg aaattgcatg
    16201 agttgccgga gtaatggtca tcggggtatc tcctttatga gtcatggtat gaagatacgc
    16261 agatttactc ttgctttaaa atgaataata ttaagccact tattcacgaa tcgagaatgt
    16321 tatggatctg acccaactgg agatgttcaa cgcggttgcc gaagctggca gcattaccca
    16381 ggctgcagca aaagtgcatc gcgtaccgtc gaatctcact acccgtctac gccagctgga
    16441 aacagaactg ggggtagatc tgtttattcg cgagaatcag cgtttacgtc tctctcctgc
    16501 cgggcataac tttttacgct atagccaaca aattctcgcg ttagtggatg aagcgcggag
    16561 cgttgtcgct ggcgatgagc cgcaaggttt attttctctt ggttcgctgg aaagcaccgc
    16621 agcagtgcgc attccggcca cgctggcgga attcaaccat cgttatccca aaattcagtt
    16681 ttcactttcc accggccctt ccggcactat gctggaagga gtgctggagg gaaaactgaa
    16741 tgcggcattt attgatgggc ccattaacca tactgccatt gacgggatac cggtataccg
    16801 cgaggagctg atgatcgtca cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt
    16861 taacggcagt aacatttatg ccttccgcgc caattgttcg tatcgtcgcc acttcgagag
    16921 ctggtttcat gctgacggtg ccgctccggg aactattcac gagatggagt cgtatcacgg
    16981 gatgttggcc tgtgtggtcg caggagcagg cattgcgctt attccacgct ctatgctgga
    17041 aagtatgccg gggcatcacc aggttgaagc gtggccgtta gctgagcaat ggcgttggtt
    17101 aacaacctgg ctggtctggc gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca
    17161 actactggat gtgcctgact cggcaaaaca gggttaccaa tgagctattt ttgatagttc
    17221 ttgcagctca aatgccctat atagtgatgt tccgatgact tatgactata tggggcaaat
    17281 atggttacgc cagtaagcat cagtaattac atatctcttc ctgatgattt tcccgtacgt
    17341 aatattgcac ctcaggtaaa agaagtttta aaggacttta ttgatgcgct aagtacaata
    17401 atatgtgatg aagagtggcg cacgtcttta aacatcaact cagccacaaa aaagatattt
    17461 aataaccttg ataacttatc atatattcag aggacttctt ttcggggtaa cgacacgcta
    17521 tacaatgaaa aggttcagtt taaactcact tatcccgtca agaatggaag acacaaagaa
    17581 aatattgaat ttcaggtagt aataaattta agccctattt atttagataa ttttcgccat
    17641 gatggagaaa ttaatatttt tgcgctccca acccaaagcc tgtcactatg gggcgcgtat
    17701 ttcagaccgg cgtcgagcgt gtgctatttc tgtttctgaa tgattttatt gaacaatttc
    17761 caatgatcaa ccttggcgtt cccatcaaaa gagcgcatac accacatatt gaacccctgc
    17821 ctccggatcg ccataccgct gcagattact tacgccagtt tgatttgctt gtcctgaatt
    17881 ttatctctcg cggtaatttt gtcatactcc cccgattatg gagtaactct gaggttcaca
    17941 gatggttcgt caataaagat cctaacttga tcaccgccat tctcgacata acagacggcg
    18001 aattaaaaga ggatttattg caaagcctga tggattcatt aggttctaac aaacatgtac
    18061 tacccgaagt ctgcatctgc tttttatccc ttttagcaga acaagaatct cctcattttc
    18121 aggacttttt tttatttttc gccaatatgt tattgcacta tcaccaattt atgaatccca
    18181 atgaaagtga tctgaatgac gtgttgatgc cagcatcatt aagtgatgat aaaattatca
    18241 aacatatggc gcgcaggacc ctcaaactgt ttgtaaaaaa tgaaacgccg ccaaaagtta
    18301 ctcacgaaga cctggtgaaa aacaggcctc gctcccctgt cagaccacct atacccgcaa
    18361 ccgccaaaac gccagacctc cctgaacgtc attaaaccgt gatgttaccg actctctgac
    18421 gcggaacaga atcagcgtca gagaaacgga aaacgcgatc cagatcacaa atgcattgta
    18481 ttcacatcat taaccgtttt aagatcattt catcactttt tcgcaactca cccgataatc
    18541 tgttatgaca acaaacactg tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc
    18601 agtcgccgcc ttcatcttca acaccaccga atttgtccct gttggcctgc tctctgacat
    18661 tgcgcaaagt tttcacatgc aaaccgctca ggtcggcatt atgttgacca tttatgcatg
    18721 ggtagtggcg ctaatgtcat tgccttttat gttaatgacc agccaggttg aacggcgcaa
    18781 attactgatc tgcctgtttg tggtgtttat tgccagccac gtactgtcgt ttttatcgtg
    18841 gagctttacc gttctggtga tcagtcgcat tggtgtggct tttgcacatg cgattttctg
    18901 gtcgattacg gcgtctctgg cgatccgtat ggctccggcc gggaagcgag cacaggcatt
    18961 gagtttaatt gccaccggta cagcactggc gatggtctta ggtttacctc tcgggcgcat
    19021 tgtgggccag tatttcggtt ggcgaatgac cttcttcgcg attggtattg gggcgcttat
    19081 cacccttttg tgcctgatta agttacttcc cttactgccc agtgagcatt ccggttcact
    19141 gaaaagcctc ccgctattgt tccgccgccc ggcattgatg agcatttatt tgttaactgt
    19201 ggtggttgtc accgcccatt acacggcata cagctatatc gagccttttg tacaaaacat
    19261 tgcgggattc agcgccaact ttgccacggc attactgtta ttactcggtg gtgcgggcat
    19321 tattggcagc gtgattttcg gtaaactggg taatcagtat gcgtctgcgt tggtgagtac
    19381 ggcgattgcg ctgttgctgg tgtgcctggc attgctgtta cctgcggcga acagtgaaat
    19441 acacctcggg gtgctgagta ttttctgggg gatcgcgatg atgatcatcg ggcttggtat
    19501 gcaggttaaa gtgctggcgc tggcaccaga tgctaccgac gtcgcgatgg cgctattctc
    19561 cgggatattt aatattggaa tcggggcggg tgcgttggta ggtaatcagg tgagtttgca
    19621 ctggtcaatg tcgatgattg gttatgtggg cgcggtgcct gcttttgccg cgttaatttg
    19681 gtcaatcatt atatttcgcc gctggccagt gacactcgaa gaacagacgc aatagttgaa
    19741 aggcccattc gggccttttt taatggtacg ttttaatgat ttccaggatg ccgttaataa
    19801 taaactgcac acccatacat accagcagga atcccatcag acgggagatc gcttcaatgc
    19861 cacccttgcc caccagccgc ataattgcgc cggagctgcg taggcttccc cacaaaataa
    19921 ccgccaccag gaaaaagatc agcggcggcg caaccatcag tacccaatca gcgaaggttg
    19981 aactctgacg cactgtggac gccgagctaa taatcatcgc tatggttccc ggaccagcgg
    20041 tacttggcat tgctagcggc acaaaggcaa tattggcact gggttcatct tccagctctt
    20101 ccgacttgct tttcgcctcc ggtgaatcaa tcgctttctg ttgcggaaag agcatccgaa
    20161 aaccgataaa cgcgacgatt aagccgcctg caattcgcag accgggaatc gaaatgccaa
    20221 atgtatccat caccagttgc ccggcgtaat acgccaccat catgatggca aatacgtaca
    20281 ccgaggccat caacgactga cgattacgtt cggcactgtt catgttgcct gccaggccaa
    20341 gaaataacgc gacagttgtt aatgggttag ctaacggcag taacaccacc agccccaggc
    20401 caattgcttt aaacaaatct aacattggtg gttgttatcc tgtgtatctg ggttatcagc
    20461 gaaaagtata aggggtaaac aaggataaag tgtcactctt tagctaacct tgcatcgcat
    20521 tgaccaaaac ttgaaccgat ttagcaaaac gtggcatcgg tcaattcatt tatttgactt
    20581 atacttgcct gggcaatatt atcccctgca actaattact tgccagggca actaatgtga
    20641 aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc catatggtta
    20701 atcagaagaa agatcgtctg cttaacgagt atctgtctcc gctggatatt accgcggcac
    20761 agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt gaactgaaaa
    20821 aagtgttgtc tgtcgacctt ggagcactga cccgtatgct ggatcgcctg gtctgtaaag
    20881 gctgggttga aaggttgccg aacccgaatg ataagcgcgg cgtactggta aaacttacca
    20941 ccagcggcgc ggcaatatgc gaacaatgcc atcaattagt tggccaggac ctgcatcaag
    21001 aattaacaaa aaacctgacg gcggacgaag tggcaacact tgagcatttg cttaagaaag
    21061 tcctgccgta aacaaaaaag aggtatgacg atgtccagac gcaatactga cgctattacc
    21121 attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc actggagaaa
    21181 gtgtcagagc gttcaggtta ctctaaatgg cacctgcaac ggatgtttaa aaaagaaacc
    21241 ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc gcaaaagctg
    21301 aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga gtcgcaacaa
    21361 actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata ccggatgacc
    21421 aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaacag ctagttgata
    21481 acgtgacaac gtcactgagg caatcatgaa accactttca tccgcaatag cagctgcgct
    21541 tattctcttt tccgcgcagg gcgttgcgga acaaaccacg cagccagttg ttacttcttg
    21601 tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt taaatcacat
    21661 gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc aacacgctat
    21721 gtagttattg tcaggctctg atcacagagc ctgacatctc cgtaatatct tcctcaagtc
    21781 tcaaatattt tcgccattgt tggcgatgag atcccgatac cagtaaaacg acgctttacg
    21841 tatccgttcc agcgttccct tcccttcgtt gtctttatcg acataaatca ttccgtagcg
    21901 ctttttcatt tcccctgttc cggcagaaac caggtcaatg cagccccacg gggtgtagcc
    21961 aattaagtca acaccatctt caactacggc ttttttcatt tcccgaatat gggaagcaaa
    22021 gtaatcaatg cgatagtgat cgttcaccgt gccgtcagct tgtctctggt caaccgcacc
    22081 aaatccattt tcgacaataa acagcggcaa ctggaaatga tcccagaacc agttgagtga
    22141 ataacgtagc cctgccggat caatttgcca tccccattcg gatgtatcga tataagggtt
    22201 gctgaccaaa tctcgtggtt caacataatc cagttgcggg ttatcgggcg aaaattgtgt
    22261 cgtaaaagac atgtagtagc taaagccgat aaagtcgaca caaccactgg caagaatcgc
    22321 gttatcttct ggtgtgatat cgaggttgaa tcctttcctg gcaaagtaat tcagcatatg
    22381 ttgcggataa tatccacgag catgaacatc agtaaaccag taacgacgat gcattgcttt
    22441 cgtggccatc atcatatcgt tgggtgcaca cgtcagagga tagatgggac acatggcgat
    22501 catacagcca atgttaaatt caggattgat cgattttcca gtctgtaccg ccagggcact
    22561 ggcaactaac tcgtaatgta ccgcctggta cattatttgt tcgcgctcat tgatatcttc
    22621 ctctggcgaa tacaagatac cggaattagt aaatggacac aggctttcgc tgaaattcac
    22681 ctgattattg atttcgttaa acgtcatcca gtactttact ttttctttat agcgcgtgaa
    22741 gaccgttgat gcgaagcgga taaaaaagtc gatcagttta cggtttcgcc agccaccata
    22801 ttttgtcacc agatgataag gcatctcaaa atgtgaaagc gtcaccacag gttccattcc
    22861 ctgcttaagg cattcatcga acagatcatc ataaaattgt aaaccctctt cattcggctc
    22921 ctgttcgtca ccttgcggaa agattcgtgt ccaggcaatg gaagttcgaa agcatttgaa
    22981 tcccatctcg gcaaataact gaatatctgt tttatagcga tgataaaaat caattgcttc
    23041 atgattggga taattaagcc cgtcgataac gccttctgtc acttcacgcg gcaccccgtg
    23101 agcgccagca gtcatcacat cagcgatgct gatgcctttt cctccttcat tccagccacc
    23161 ttccaactga tgcgcggcta ccgcaccgcc ccataaaaaa ccttttttaa atcctgacat
    23221 acacaactcc ttaactaaaa tagattacca ccagacttcc gcctggatgc cggccataaa
    23281 ctcgtctttg ctgttctcat taaacttaaa attcgataat tcgttatcca gaatattgat
    23341 atacgttcca tagaagcgaa tttctggtcg tgaacctaaa atgctttcac ccactttcaa
    23401 tgcatgccag agtgtcgttt tataagccga ttcatttagt gttacaccct gctgagtttt
    23461 gttctgttgc ttaaaccagc ctaattcaag ccccgtctga ttccatgtat tccagatcca
    23521 ggccggtctt attacagtgc gaatactgtc aaaatcacta tgagcaccac tttcataact
    23581 ataaacatct tcgccgtgag aatagacaag cgcgttagcc ataataatat tgtcagtaag
    23641 atacatctcg ccctgagaga ttaaacgcca ggcgatccca ttggtatgat caccatagta
    23701 atagcgacca tgcgccatcg tattactggc atctgagaaa ctggcaaaac tgctggcata
    23761 ggaattattg gcaacctgta acgtaaattc attaaacgta tcgcgttgca ggttttgccg
    23821 taaaacagaa gtcagcatcc agctatcttt catttcaaaa taactgttgt cattttcgtt
    23881 atcttgttgt tgatccgttt tattaggtgc ggaatattta gccattaatg ataatgtcgc
    23941 atcatcccat aacgggatat tgcgataacg aacatccaca gaattggtat tcatctggct
    24001 ggtacgcgaa aaatcacgag agtaaacatc gacatcatca cggcttaagg acatatcaaa
    24061 cagccctaca ccaagtgccc agttttcaat ccccacaccc gcggcgacat ccgtggttaa
    24121 ggttttccag tccagcatct ggatctcata ttgcgggagt ttatgtttgc cgacccagaa
    24181 gtctgcctct ggtgcgaagg gtaaaaaacc tcgcgttgtc aggtaaatat cgctgaactg
    24241 catgatattt tcattggcac tgtcaccaaa ccaggcatcg ttatactgct cacccacgtt
    24301 gccatcatag gtaacgaccg catttgccgt tttaccgtcc tgattataaa cacgctgatt
    24361 taaggtcagg tcaaaccaac cgctcatctc attaccaaaa cgtcccagag aacccgctgc
    24421 ataagtttgt ggtgagccgt gattaccagt accccagcct gagcggaagt acccctgata
    24481 gctgaaccca atatcatcct ttatatattt actgatatct ttcagggtca cgctttcgat
    24541 agtggttttg tcgcctttac tttcaataac ggcaacttgc tgcgatccat ttatagtatg
    24601 cgctgcggta aacgagttct gttcaccgtt ttcatttttg atgttatcag cgacgggaga
    24661 tgtcgctgat atttcagcaa gcgaggctga tttatatttg ttttccgtga tgcttttttg
    24721 taaggtcgaa agtcgggatt tatatactcc cagttcattc tgtgttgctt tcagctcttg
    24781 tttattttgc gacaattcat tttcaagcaa ttcaaggcgc tgctctatgg ttaattttgt
    24841 tgccatcgcg ttcaatggaa acaatgctgt aataatagaa aaagacaaaa ggctgacgtt
    24901 aagcgtcttt atattcatga cttatcctta agagtataat aatttcctcc agctatcgca
    24961 ctggaggaaa aaatcaggca tggagttctt ttcttaatga aataatttcc ctggctaaat
    25021 cctggcataa tattgcattc atcaaatgat cctgtgcatg aatcagaatt aaattaactt
    25081 ttattttgcc acagccttca tcctgactaa ttaatgcggt ctgaatctta tgtgcctgaa
    25141 gacaggcaga ttcagaactg gccattagct gagttgcgcc ctgccagtct ttttgcccgg
    25201 cacaacgtat ggcttccatc gcgtcagaac gtgcctgccc cgcgttgacc agtaactcca
    25261 taacaagttc ttcatctgca aacatcacaa tctccttcct gggtaaatgg aattatccgt
    25321 tagttgtcat gttattgact ttgttggcgg caataacaaa aggtaaatag atcattgtcg
    25381 ccacacctaa acataaaaaa ccaaccagta aagccagcca gttccccccg gttccgagga
    25441 aagcaattaa aggtccgggc gtagtccagg gcacggcata agcaaccggt ggaataattt
    25501 ccatcgaaac aaagaagtaa ccaatggcac agttaacaat gggaaccata ataaacggca
    25561 ccatcatgat ggggttaagt acaataggaa ggtcaaacat tatcggttca ttgatattga
    25621 aaataccggg gataaatgac atttttgcca gatcacggta ttccgcacgc ttagaagcga
    25681 taaaaatagc caacaataac cccaacgtca tacctgaacc gccgcagttg gcgaatgcat
    25741 cattaataga agtccaggta atcggatatg gcgcgcccca ggtagtgcca tgttgagcgg
    25801 cccaggagag attctccaga ttagcctcag aaaaaacggt ttctcgaatt gccgatgtgg
    25861 tattaggtcc gtggatcccc agcacccaca ggaaattacc cacagccccc aggataatta
    25921 ccgcaaagat attcgttccc atatgtttga gcggcgtctg gattaatgtg taaataagat
    25981 cgtttagtcc tgccggggat atcagtgtca ggcaataatt aagtgcggaa aagaacacca
    26041 tgacaaaaaa tattggcaat aaaactttaa atgaacgcgc taccgcaggc ggtacagctg
    26101 ccggcatcgt tatggtgatt tttgggtttc gagctaagcg acaaaatatt tcactggtga
    26161 tcaatgcaac gataacagca acaaacaacc cctgcgcgcc gagatatttg gtcgtcagga
    26221 atgcttgccc atctatcatc tgatatggcg tataaacaat aaaaaatgct cctattgccg
    26281 ttaatccgca taaaagatca tcttgctcat agctaatcgc catattcctg gcgaccagga
    26341 aagcaatcat aattgacatg atatttacag aaccattcat taccggagta aaaatagctt
    26401 gtgctttttc aaggttgggg aaaatgctat tcagatgcag taatccggca ataaaaccgt
    26461 ctggcgataa tatggcaaag ttaattaata taattaatga acttgccatg ataattggaa
    26521 acgtaaaaac gaatccatct ctcaccgctg caacatgttt ttgtgagttt aacttgatag
    26581 caactggaac aagaaaacgt tccattccac gttcgaatga ggccattaat cccatttttt
    26641 tatacctgtt gttttggata tttatacatc ccaataaagc atgtatgcca tatccatttt
    26701 atgatgtgtg aaaagttagt tcaggtaata gtttaataat attagattct tactcttgca
    26761 gtagtttcat cgcaaattca agaactgcct gtccgttcat ggtgccgtag tctttcatct
    26821 cgataacggc tacaggcatt ttcccctgtg caacggcttc aatctctggt ttctgaaagc
    26881 gaacttgcgg gccaagtaaa acaacgtcaa cttcgttatt tttaattttc cctttagcct
    26941 ctgcaatcgc caatgcggaa atattaactt caagtgaaat agcgttagca tgatcaatca
    27001 tacgtttaac cagcatactg gttgacatgc ccgcagcgca aactaacaat atttttttca
    27061 tattttccct tactggtata taacagatta cattgtttaa atatcatatc ccgcagtagc
    27121 tatacgtcct ggtacattga tcacagtaat acagtgataa agttgttcaa catcagggtg
    27181 attattaaac gactcggcca tacgataaac caatcaggac tcaactcctt gttttcaatg
    27241 gctcttaaaa ggtgcaaaag tgatttttca aaagattgcc cgcttactta aatccgaaat
    27301 caatggcaat tcatggcatg ttggtgattt gttgccgtca gaagcggaac tcgctgttcg
    27361 ttataatgtt tcgcgtaata ccctgcgtaa ggcattatcc ttgctggaag gcgaaggaat
    27421 tattcacaga aagcatggtt caggaagata cattcaaaaa aagaattttg ttgcacacat
    27481 tgatcacatg aacagtttca gtgaaattgc gcataaaagc ggcaaagagg cagggagtca
    27541 gattatgaaa tttgaagtgc aggatgcttc acccactatt gcgactgagc tgaatttagt
    27601 gactggtgag caggtttatt acataaagag actgcgattc attgaggata atgcagcaca
    27661 attagaagaa acgtggatgt cagtagcacg ttttcctgat ttaaccgtgt cacatatgca
    27721 aaaatccaaa ttttcatata ttgagaatga atgcgggatc aaaatcattg gcacctttga
    27781 aactttctcc cctacttttc ctaccccaga aatcgccagt attttacgga tcagcccacg
    27841 ggatcccata cttaaaattc agacccaggc tgtggacagt aactctattc cgctggatta
    27901 ttcgttactt tacagcaata ttttcgagtt ccaggtaaag tactttttcc cgcgataaaa
    27961 gcagacgcac agagtgctga catcacattc tgtgcgtatg gtttagcctc taacctttac
    28021 cgctttacgc caccgcaagc caaatacatt gatatacagc ccggtcataa tgagcaccgc
    28081 acctaaaaat tgcagacccg ttaagcgttc atccaacaat agtgccgcac ttgccagtcc
    28141 gactacgggc accagtaacg ataacggtgc aacccgccag gtttcatagc gtcccagtaa
    28201 cgtcccccag atcccataac caacaattgt cgccacaaac gccagataca tcagcgacaa
    28261 gatggtggtc atatcaatag taaccagact gtgaatcatg gatgcggaac catcgagaat
    28321 cagcgaggca acaaagaagg gaatgattgg gattaaagcg ctccagatta ccagcgacat
    28381 caccgccgga cgcgttgagt gcgacatgat ctttttattg aagatgttgc cacacgccca
    28441 actaaatgct gcagccaggg tcaacataaa gccgagcatc gccacatgct gaccgttcag
    28501 actatcttcg attaacacca gtacgccaaa aatcgctaag gcgatccccg ccaattgttt
    28561 gccatgcagt cgctccccga aagtaaacgc gccaagcacg atagtaaaaa acgcctgtgc
    28621 ctgtaacacc agcgaagcca gtccagcagg cataccaaag ttaatggcgc agaacagaaa
    28681 ggcaaactgc gcaaaactga tggttaatcc ataccccaag agcaaattca gtggtacttt
    28741 cggtcgtgca acaaaaaaga tggccggaaa agcgaccagc ataaagcgca aaccggccag
    28801 catcagcggt ggcatgttat gaagccccac tttgatgacc acaaaattta gcccccatac
    28861 gaccactacc agtagcgcca acaccccatc ttttcgcgac attctaccgc ctctgaattt
    28921 catcttttgt aagcaatcaa cttagctgaa tttacttttc tttaacagtt gattcgttag
    28981 tcgccggtta cgacggcatt aatgcgcaaa taagtcgcta tacttcggat ttttgccatg
    29041 ctatttcttt acatctctaa aacaaaacat aacgaaacgc actgccggac agacaaatga
    29101 acttatccct acgacgctct accagcgccc ttcttgcctc gtcgttgtta ttaaccatcg
    29161 gacgcggcgc tacgctgcca tttatgacca tttacttgag tcgccagtac agcctgagtg
    29221 tcgatctaat cggttatgcg atgacaattg cgctcactat tggcgtcgtt tttagcctcg
    29281 gttttggtat cctggcggat aagttcgaca agaaacgcta tatgttactg gcaattaccg
    29341 ccttcgccag cggttttatt gccattcctt tagtgaataa cgtgacgctg gtggtgctct
    29401 tttttgccct tattaactgc gcctattccg tttttgctac cgtgttgaaa gcctggtttg
    29461 ccgacaatct ttcgtccacc agcaaaacga aaatcttctc aatcaactac accatgctaa
    29521 acattggctg gaccatcggt ccgccgctcg gcacgctgtt ggtgatgcag agcatcaatc
    29581 tgcccttttg gctggcagct atctgttccg cgtttcccat gcttttcatt caaatttggg
    29641 taaagcgcag cgagaaaatc atcgccacgg aaacaggcag tgtctggtcg ccgaaagttt
    29701 tattacaaga taaagcactg ttgtggttta cctgctctgg ttttctggct tcctttgtta
    29761 gcggcgcatt tgcttcatgc atttcacaat atgtgatggt gattgctgat ggagattttg
    29821 ccgaaaaggt ggtcgcggtt gttcttccgg ttaatgctgc catggtagtt acgttgcaat
    29881 attccgtggg ccgccgcctt aacccggtta acatccgcgc gctgatgaca gcaggcaccc
    29941 tctgtttcgt catcggtctg gttggtttta ttttttccgg caacagcctg ctattgtggg
    30001 gtatgtcagc tgcggtattt actgtcggtg aaatcattta tgcgccgggc gagtatatgt
    30061 tgattgacca tattgcgccg ccaggaatga aagccagcta tttttctgcc cagtctttag
    30121 gctggcttgg tgctgcaatt aacccattag tgagtggcgt agtgctaacc agcctgccgc
    30181 cttcctcgct gtttatcatc ttagcgttgg tgatcattgc tgcgtgggtg ctgatgttaa
    30241 aagggattcg tgcaaaaccg tgggggcagc ccgcgctttg ttgatttaag tcgaacacaa
    30301 taaaaattta attcagcctt cgtttaggtt acctctgcta atatctttct cattgagatg
    30361 aaaattaagg taagcgagga aacacaccac accataaacg gaggcaaata atgctgggta
    30421 atatgaatgt ttttatggcc gtactgggaa taattttatt ttctggtttt ctggccgcgt
    30481 atttcagcca caaatgggat gactaatgaa cggagataat ccctcaccta accggcccct
    30541 tgttacagtt gtgtacaagg ggcctgattt ttatgacggc gaaaaaaaac cgccagtaaa
    30601 ccggcggtga atgcttgcat ggatagattt gtgttttgct tttacgctaa caggcatttt
    30661 cctgcactga taacggatcg ttgacacagt agcatcagtt ttctcaatga atgttaaacg
    30721 gagcttaaac tcggttaatc acattttgtt cgtcaataaa catgcagcga tttcttccgg
    30781 tttgcttacc ctcatacatt gcccggtctg ctcttccaat gaccacatcc agaggctctt
    30841 caggaaatgc gcgactcaca cctgctgtca cggtaatgtt gatatgccct tcagaatgtg
    30901 tgatggcatg gttatcgact aactggcaaa ttctgacacc tgcacgacat gcttcttcat
    30961 cattagccgc tttgacaata atgataaatt cttcgccccc gtagcgataa a
//
LOCUS       DAZFJF010000048        30913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFJF010000048 DAZFJF010000000
VERSION     DAZFJF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.048"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            185..913
                     /gene="gadW"
                     /locus_tag="ACHKAU_002372"
     CDS             185..913
                     /gene="gadW"
                     /locus_tag="ACHKAU_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO5531426.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1276..4389)
                     /gene="mdtF"
                     /locus_tag="ACHKAU_002373"
     CDS             complement(1276..4389)
                     /gene="mdtF"
                     /locus_tag="ACHKAU_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO5531427.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4414..5523)
                     /gene="mdtE"
                     /locus_tag="ACHKAU_002374"
     CDS             complement(4414..5523)
                     /gene="mdtE"
                     /locus_tag="ACHKAU_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO5531428.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5631..5909
                     /locus_tag="ACHKAU_002375"
     CDS             5631..5909
                     /locus_tag="ACHKAU_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531429.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5910..6437)
                     /gene="gadE"
                     /locus_tag="ACHKAU_002376"
     CDS             complement(5910..6437)
                     /gene="gadE"
                     /locus_tag="ACHKAU_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5531430.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7236..7808)
                     /gene="hdeD"
                     /locus_tag="ACHKAU_002377"
     CDS             complement(7236..7808)
                     /gene="hdeD"
                     /locus_tag="ACHKAU_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO5531431.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            8063..8395
                     /gene="hdeA"
                     /locus_tag="ACHKAU_002378"
     CDS             8063..8395
                     /gene="hdeA"
                     /locus_tag="ACHKAU_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO5531432.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            8511..8837
                     /gene="hdeB"
                     /locus_tag="ACHKAU_002379"
     CDS             8511..8837
                     /gene="hdeB"
                     /locus_tag="ACHKAU_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO5531433.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            8901..9548
                     /gene="yhiD"
                     /locus_tag="ACHKAU_002380"
     CDS             8901..9548
                     /gene="yhiD"
                     /locus_tag="ACHKAU_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO5531434.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(9590..10120)
                     /gene="dctR"
                     /locus_tag="ACHKAU_002381"
     CDS             complement(9590..10120)
                     /gene="dctR"
                     /locus_tag="ACHKAU_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5531435.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(10276..10842)
                     /gene="slp"
                     /locus_tag="ACHKAU_002382"
     CDS             complement(10276..10842)
                     /gene="slp"
                     /locus_tag="ACHKAU_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO5531436.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(11197..12312)
                     /locus_tag="ACHKAU_002383"
                     /pseudo
     CDS             complement(11197..12312)
                     /locus_tag="ACHKAU_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12892..13893)
                     /locus_tag="ACHKAU_002384"
     CDS             complement(12892..13893)
                     /locus_tag="ACHKAU_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5531437.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            14000..14296
                     /locus_tag="ACHKAU_002385"
     CDS             14000..14296
                     /locus_tag="ACHKAU_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5531438.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(14430..14855)
                     /gene="arsC"
                     /locus_tag="ACHKAU_002386"
     CDS             complement(14430..14855)
                     /gene="arsC"
                     /locus_tag="ACHKAU_002386"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO5531439.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(14868..16157)
                     /gene="arsB"
                     /locus_tag="ACHKAU_002387"
     CDS             complement(14868..16157)
                     /gene="arsB"
                     /locus_tag="ACHKAU_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO5531440.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(16211..16564)
                     /gene="arsR"
                     /locus_tag="ACHKAU_002388"
     CDS             complement(16211..16564)
                     /gene="arsR"
                     /locus_tag="ACHKAU_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO5531441.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            17303..17386
                     /gene="dinQ"
                     /locus_tag="ACHKAU_002389"
     CDS             17303..17386
                     /gene="dinQ"
                     /locus_tag="ACHKAU_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO5531442.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(17440..18792)
                     /gene="gorA"
                     /locus_tag="ACHKAU_002390"
     CDS             complement(17440..18792)
                     /gene="gorA"
                     /locus_tag="ACHKAU_002390"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO5531443.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(18864..19706)
                     /gene="rlmJ"
                     /locus_tag="ACHKAU_002391"
     CDS             complement(18864..19706)
                     /gene="rlmJ"
                     /locus_tag="ACHKAU_002391"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO5531444.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            19909..21951
                     /gene="prlC"
                     /locus_tag="ACHKAU_002392"
     CDS             19909..21951
                     /gene="prlC"
                     /locus_tag="ACHKAU_002392"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO5531445.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            21959..22711
                     /gene="rsmJ"
                     /locus_tag="ACHKAU_002393"
     CDS             21959..22711
                     /gene="rsmJ"
                     /locus_tag="ACHKAU_002393"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO5531446.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(22760..24229)
                     /gene="dtpB"
                     /locus_tag="ACHKAU_002394"
     CDS             complement(22760..24229)
                     /gene="dtpB"
                     /locus_tag="ACHKAU_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO5531447.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            24395..24448
                     /locus_tag="ACHKAU_002395"
     CDS             24395..24448
                     /locus_tag="ACHKAU_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531448.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(24547..24981)
                     /gene="uspA"
                     /locus_tag="ACHKAU_002396"
     CDS             complement(24547..24981)
                     /gene="uspA"
                     /locus_tag="ACHKAU_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO5531449.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            25372..25707
                     /gene="uspB"
                     /locus_tag="ACHKAU_002397"
     CDS             25372..25707
                     /gene="uspB"
                     /locus_tag="ACHKAU_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO5531450.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(25952..27451)
                     /gene="pitA"
                     /locus_tag="ACHKAU_002398"
     CDS             complement(25952..27451)
                     /gene="pitA"
                     /locus_tag="ACHKAU_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO5531451.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            27683..28885
                     /gene="yhiN"
                     /locus_tag="ACHKAU_002399"
     CDS             27683..28885
                     /gene="yhiN"
                     /locus_tag="ACHKAU_002399"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO5531452.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(29201..30253)
                     /gene="yhiM"
                     /locus_tag="ACHKAU_002400"
     CDS             complement(29201..30253)
                     /gene="yhiM"
                     /locus_tag="ACHKAU_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO5531453.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            30636..>30913
                     /locus_tag="ACHKAU_002401"
     CDS             30636..>30913
                     /locus_tag="ACHKAU_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531454.1"
                     /translation="MKAIDNIFKYVPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNDRSHQMEKVWFYNSEPSIDI"
BASE COUNT         8281 a         7274 c         7462 g         7896 t
ORIGIN      
        1 cgctgtgcga aaatatattc gtgctgcatt tacttattat caattaactg ttatgcaaaa
       61 ctactttgtg gataaatttt ggtcctacca aatctggcag tttttgcgct aagaaacagt
      121 ctggcaacat ttcattagta tactgaaatt gaaataatcg cagtatgaaa tataagggat
      181 aatcatgact catgtctgct cggtgatcct cattcgtcgt tcattcgata tttatcatga
      241 acagcataaa atatcgctgc ataacgagag tatcgtgctg ctggagaaaa atttggcaga
      301 cgattttgcg ttttgttcac cggatacgcg acgactggat atcgatgagc tgacagtttg
      361 ccattactta caaaatattc gtcagctacc acgcaattta gggttacata gcaaagaccg
      421 tctattaatt aaccagtcac cccccatgcc gctggtgacg gcgatttttg atagcttcaa
      481 tgaatccggg gtaaattcac cgatactgag caatatgctc tacctttcct gtttatcgat
      541 gttttctcat aagaaagaac tgatcccctt acttttcaat agcatcagca ctgtttcagg
      601 aaaagttgaa cgccttatta gctttgatat cgccaaacgt tggtatctgc gcgatatcgc
      661 ggaaagaatg tataccagcg agagtctcat caaaaaaaag ttgcaggatg aaaatacctg
      721 tttcagtaaa atattactcg cttccaggat gtcgatggcc agacgattac tcgagttacg
      781 tcaaattcct ctgcatacta ttgcagaaaa atgtggctat agcagtacgt cgtactttat
      841 aaacacattt cgacaatatt atggtgtaac gccacatcag tttgcgcaac attcgccagg
      901 taccttttcc tgacatattt tgcatttgaa tattggtcag gatctcacac ctgcttcatg
      961 tgaaactctt ccctgatgat ttctgccggg ctaccggcta gttctctttc gcagtttgcc
     1021 aggcggtata ggtattttca ggaggaaaag actcggcatg tttgggatta ttaagctgac
     1081 aattcatacg attacgggaa tgtgggtcag tattgtgctg ttcaaattga tgacgaatgg
     1141 ctggagtggt ttctattttc agtgctgtgt actaagcctg gtattcctga cggttagctg
     1201 gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct gaaccttcac gttcaacctt
     1261 actctcattt acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa
     1321 aaagaccgga acgaagtaaa ttgccagcac tgttgcggca aacatcccgc ccatcacgcc
     1381 ggtacctacc gcgttttgcg cgccagaacc ggcaccatga ctgataacca gcggcagcac
     1441 gccgagaata aaggccagag aggtcatcag gattgggcgt aaacgcatcc gcgccgcttc
     1501 gatgattgcc tctatcggcg ttttcccttc tttctgcatc atctcaacgg caaattcgac
     1561 aatcaggatg gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta
     1621 gacgtcatta cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg
     1681 aacaaccaac atcaccgaga acggaattga ccagctctca tagagtgcgg cgagggcgag
     1741 gaacaccacg accagtgaaa tggcatacag tgcaggagcc tggtttgagg ataacgcttc
     1801 ctgatacgac agtccggtcc atgagtagcc gacgcctgcc ggaagtttag cgaccaggtc
     1861 tgccataaat ttcatagcgt caccggtact tttcccggcc gccgcttcac ctaaaatctc
     1921 cattgacggg atgccgttgt agcgttccaa tcgcggtgaa ccataggtcc attcggtaga
     1981 cgagtaggca gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat
     2041 gttatccggc aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc
     2101 ctggttgagg aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat
     2161 atcagacagc gccacaccca ttgcctcagc tttcgcggcg ttgacgttca ctttgaacat
     2221 cggcgtatct tccaggccgt tcgggcgtac cccggtgacc tggtcaggcg attgcgctgc
     2281 cagtgataac agctcgtttc gtgcctgggt tagtttttcg tgccccaggt taccgttgtc
     2341 cagcagttcc atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa
     2401 cgggaagacg acggctttat tgatactgct taacgcaatc attgcccgct gaatgatcgc
     2461 ggtaaccgag ttttcctcac cgacacgttc agaccacggc ttgagactga taaacgccag
     2521 gccgttgttt tgcccctggc cgctgaagcc aaagccgcca acggtaaaca ccgactgaac
     2581 attatctttc tcttttgtaa gataataatc cgtcacctgt tgcagcactt tcgtggtgtt
     2641 aaccatggtg gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc
     2701 tggtaagaaa gaggtcggtg tacgcaggaa cagcaccgcc atcccggcgc aaatcagcag
     2761 gtagaccacc atgtagcgac cggtacaacg caacagcgag cgggtgctat cggtatagtg
     2821 ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgacc
     2881 gccttccggc gcggctttca gaatggtggc gcacagggca ggggtcaggc tcattgccac
     2941 aaatactgaa agcagcatgg aggagatcag cgtgatggag aactggcggt agatctcccc
     3001 ggttgcgccg ctcataaagg ccatcggcat aaacactgcg gaaagaacaa cagcaatacc
     3061 gaccagcgca cgttggatct gccccatcga tttatgcgtc gcttccttcg gtggtagctt
     3121 atcttccgca atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa
     3181 ccctatcgcc agcaccatcc cgaacatagt taacgtgttg atggtgaaac cgaccgccga
     3241 caagatcgca aacgtcccga gaataaccac cggtacggca atcgtcggga tgattgtggc
     3301 acggaaattc tgcaaaaaca gatacatgac caggaagacg aggatgatag cctcaaccag
     3361 tgttttaaaa acttcctgaa tagaaatttc gataaacggc gtggtgtcgt aaggataaac
     3421 cgtcttcaga cttgcgggga aataggctga taagcggttc agctcctctt tgactgcccg
     3481 cgaggtatcc agggcgtttg ctccggtagc cagtttgatg gcgatcccgg cagcaggttt
     3541 gccgttatag cgcgccacgg tggaataatc ttccgctcca agttcgacgc gggcgacatc
     3601 acgcagcagc acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc
     3661 cggcgtttgc agacgcgtct gcacaatgat cgaggcgttt agttgctggt ctgccgcctg
     3721 tggcatgcca cccagttgac caccggaaat ctggttgttt tgcaccttaa tctgggaaat
     3781 aacatcggaa ggtaccagat tgtatttatt gagtttttgc gggtccagcc agatacgcat
     3841 ggcgtactcg gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc
     3901 tttgatatta gacgctacat agtccgcgat atcgtactgg ttgaggctgc cgttatcaga
     3961 aataaacgcc gctaccatca gaatattact gctcgactta tcaacgctaa tcccctgctg
     4021 ctgcaccgct tcaggtaatg aaggcatagc gagttgcagt ttattttgca cctgaacctg
     4081 tgcgatatca ggagatgtcc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc
     4141 atcactggtt gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac
     4201 ctgagtgacc gagtcttcta ccgtttgcgc atcggcacct ggataggtag cgctgacggt
     4261 aatggtcggt ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc
     4321 acctgcaagc atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata
     4381 gttagccatg aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt
     4441 ttcctggctg gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga
     4501 aacgatcacc cgatcgccag cctgcaagcc agaggtgaca acccactggt cgccgatggc
     4561 tttgctggct tcaatttcgc gtagctgcac aacatcgtct ttatccagaa tgagcgccgt
     4621 tgctttaccc tgggcgttgt gggtgacgcc ttcctgcggc accagtaata cattctggcg
     4681 gctaccttca tccactaatg ccgtgacgta catgccaggc agcaagtcac catttgggtt
     4741 ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca tccaccgtcg ggtcggagaa
     4801 tttcagcgtg ccggtctggc tgtagcgttt accattttcc agattgagct gtactggcgt
     4861 actgccctga acctgtttga tttgcccact ggcgacctct tctttcatgc gtaagaaatc
     4921 ttgcaccgac tgcgtgagat cgacataaat cgggtccaga cgttgtacgg taaccagcga
     4981 atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc gacgatttcc cgctgacgcc
     5041 cgtaatcggc gaggtgacat tcgcgtattg cagattgatc gtcgcctgtt caacagccgc
     5101 tttggcgacg gtgacattgg cttctgcttc attcaactgg gtgcgcgcgg tatcgtaatc
     5161 ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg cggttaaagg tgatgcgggc
     5221 attgctggcg gtagagagcg ctttcgccag cgagcctttg gcggagttta gctcggcctg
     5281 taaaggtgca ggatcaatct gatacagcga atcgccctgg ttcactttat cgccttcgat
     5341 aaagttgcgt ttaatgataa taccgcccac ctggggacgt atctcggcaa cttcataagg
     5401 aacggttcta ccgggcaatt cgctcaacac attgaccgaa ccgggggaga gtgtgacgac
     5461 accgacctca ggcgtcatgg cggcggcgtt ttccgccgat ttgtcatcgc aggcggtgag
     5521 catcgcgccg cagaataaca acggtattaa cagctttctt cttctgttca ttttagtccc
     5581 tgaaaattct tgagaaataa cggttcttta aattgcgcgc gaggagagat atgagggatt
     5641 tacagaccag cggcatagtg gggttgtcag ccagtaaggt gggatacagg cacagtgatc
     5701 gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct gccaacagtt cctgccagca
     5761 ttcgggcaac gaagggttct actggtggat acacatacca ggggaataaa gtcgttttag
     5821 cctctccttt agtaatcacc gggggtaatg aaatcagcat ctgcatccct cgtcatgcca
     5881 gccatcaatt tcagttgctt atgtcctgac taaaaataag atgtgatacc cagggtgacg
     5941 atgtcgctca tacgtcttag tttcagcttg tacataatgt tgaatttctg aatctttaat
     6001 gtttgttgcg aaatattgag cgtcgacgtg atattgcttt catttttatt tttcagaatc
     6061 agttgcataa ttttgaactg attatgtgaa aaacataatt gtgaattctt atggggcaag
     6121 tgtttaccat aagtaatctc aagaagattt ttgatacttt ctttgcggct aacaaaataa
     6181 ataggcgctt tagcttttag tttactgatg tgataaggtg ccaaaacaat aattctctcg
     6241 gcatctaatt tctccagaaa tttaatcgct tcttcatcaa ggatatgatt gtgataggta
     6301 tcaatgataa ccttgaaggg tttattgcct acttttttga tctctgacag ggaattgatt
     6361 tttatcattt cgtgattatc tttcaactgc caaaagccct gtaaaagaaa agaatctttc
     6421 gtcatgagaa aaatcataac ttgctcctta gccgttatcg tttcgaacat gtcatccttt
     6481 tcattattta catccttgtc cgaatcgttg ttcaatatag taaacgccta taagaacttt
     6541 gcccttcgtg aactaatacg tctattaaca ctaatcaata cttcagattt atccatcacg
     6601 cactccatat attgatccta agtaagattc ctggttgtta tcaatttgta aatatcaaac
     6661 aaaaagttaa cgtttgcgga ttactggcca agaataagtt atcaataacc agtttgtgat
     6721 ctctgaagaa tattactaaa gttaaaatgt aatccgattt aaatatcgag tctccttgtt
     6781 tcgacttaag ctggcaattg gattgccagc tttcttttaa cgcattgaaa ttacctgagt
     6841 aaataacgca ttcatgtctt caggttttta tatagcgtct ggagacacgg atatttatgc
     6901 aatgtaatca acgccagttg caaaaaaatg aacccggatt acttatttaa ccgcccatag
     6961 catcaacgct tacggaactc tttgttcact ttcccacagc acttttaaaa cattaaacct
     7021 gactacggaa aatatcagcc atgataatgt ttgaaatggc taatttgcca tggagtgaaa
     7081 aaaattagat gaaattcagt aggttgaaat gatcactagc aggtaattat ttcaatgata
     7141 gtgcgcaatt gatctacaac actgcgtagc ggagagagta ttaatcggat catagtcaca
     7201 tcaagtgact atgatcctgg tgacaaccgg gataattatt gctgcttaac gaacaaactg
     7261 gcgaagctga acaggctggc ggcgctaaat atcagttcaa ttcccaccag tgtggaaacc
     7321 agcgttacag acaccatcgg cgttgcacca aggaatatcc aggcaatgac gatatccagc
     7381 acaccaataa cgagctgtag ccagctgcct ttcattgaac gctgacgata ccaactcatc
     7441 aggcgaataa cccctgcaac acagaacaaa ccggcaataa atgccgcaat ggcaaaaatg
     7501 cccagctccg gtgcgcggat gaagaaatag ccgatcaata aataggcgac tgcgacgagg
     7561 aaaccggata ataccggcca gaaattatga ctgcggttgc tgaataaccc gacaataagc
     7621 gcaatacccg agcagattaa taatgcaccc actactgtgc ttaaaatatc gccagagacg
     7681 aacgggaaac tgatacacag caacccgacg ataaacagca gcacggcaat aaactggatt
     7741 gctctgcgat gctttttcag catctccaga tcaaacttca aaattgttgc cttatctata
     7801 tataacatag aaccacccta taaaattaag aagaaaatcc cctgctatca atctatgcca
     7861 aaaacgcgtc taagaatgca gtcgatttaa taaaaatttc ctaattgcag tatctgatgc
     7921 atctgtaact cattgtattg aaataaaaat atctgatttt gatattttcc atcaacatga
     7981 catatacaga aaaccaggtt ataacctcag tgtcgaaatt gattcgtgac ggctctttca
     8041 ctttatagtt gaggatatta cgatgaaaaa agtattaggc gttattcttg gtggtctgct
     8101 tcttctgcca gttgtgagca atgcagcgga tgcgcaaaaa gcagctgata acaaaaaacc
     8161 ggtcaactcc tggacctgtg aagatttcct ggctgtggac gaatccttcc agccaactgc
     8221 agttggtttt gctgaagcgc tgaacaacaa agataaacca gaagatgcgg ttttagatgt
     8281 tcagggtatt gcaaccgtaa ccccagctat cgttcaggct tgtactcagg ataaacaagc
     8341 caactttaaa gataaagtta aaggcgaatg ggacaaaatt aagaaagata tgtaattccg
     8401 ggaatgcgtt acatcgtact tccttgcata ttgaacaggc cggaatatct tctttaaaag
     8461 cagctattcc tcctgttcat atataatctc tatattgaat gggttacaaa atgaatattt
     8521 catctctccg taaagcgttt atttttatgg gcgctgtagc ggctttgtca ctggtgaacg
     8581 cacaatctgc gctggcagcc aatgaatccg ctaaagatat gacctgccag gaatttattg
     8641 atctgaatcc aaaagcaatg accccggttg catggtggat gctgcatgaa gaaacagtat
     8701 ataaaggtgg cgataccgtt actttaaatg aaaccgatct cactcaaatt cctaaagtga
     8761 tcgaatactg taagaaaaac ccgcagaaaa atttgtatac cttcaaaaat caagcatcta
     8821 atgacttgcc gaattaatga ggtacaagta aaaaggagta gcaagttgag ccatcttgct
     8881 gctccttttt gcatttttat atgacagcag aatttattat acgtcttata ctcgcggcaa
     8941 ttgcctgtgg cgctattggc atggaaaggc aaatgcgcgg caaaggagca gggttacgca
     9001 cacatgtatt aattggcatg ggaagcgccc tgtttatgat tgtttcgaaa tatggttttg
     9061 ctgacgtgct gtctttagat cacgtcggac tcgaccccag ccgtattgct gctcaggtgg
     9121 tgacgggcgt cgggtttatc ggtgcaggta acattctcgt tcgtaaccaa aatattgtag
     9181 gtctgacgac ggcagcggat atctgggtga ccgccgccat aggtatggtt attggcagcg
     9241 gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg gaagtcttcc
     9301 atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca ttagtgaacg
     9361 gtaatacggt atcaatgctc gactggttta agcagcaaaa aataaaaacg gatttagttt
     9421 cgttgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt catgccacaa
     9481 cgtcgataga agacctgttg cgattattaa aaggtatagc aggggtgaaa ggcgtttcaa
     9541 ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt cacaccagat
     9601 aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg atcatcatga
     9661 tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc agaatatctt
     9721 cagttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc gaaagtgaca
     9781 cattcgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa tcaatgatag
     9841 tgatagtccg cggaacaaag gtcaccggta ctttccccag acagcgttta atattaaagg
     9901 gggagaaaac aatgacatgg acaggtttta atcgttcaat ttgagtgaga aaattagaat
     9961 gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc aacattacgt
    10021 cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg tttttcatcg
    10081 cggtgaagaa catcgtatcc ctggtaatta taagaaacat aagcgactcc tggtgcggac
    10141 attaacttta taatcagttt gccaaaggta attaacagga cagggcttat aataataata
    10201 gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag aaagcatctg
    10261 catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg cattggtgta
    10321 gtaaggcgca ccccagcccg gttccggcca gaatgcacca tagccgtaat cccacaggtt
    10381 ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca tattcacttc
    10441 caggaagtta tacgggactt tattgataaa gcctggttgt tcaccctgaa tggtaccgag
    10501 gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt gtctggcgag
    10561 cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca acggtaatac
    10621 agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct tcccaccaaa
    10681 gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa aacttttttg
    10741 aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg cggcaagcaa
    10801 aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat ccttatcaac
    10861 aaatattaaa gccataatag atatttcttt atagttttca tctgattctg aggtgatatg
    10921 cctgatagta acgtaaacct gtctgatgtt tatatccctt ttgtttagtt caattccatt
    10981 gattttgggg cgtggcatga gtatccatcg ccccataata taatcctttg ttttaaaatt
    11041 acataaaatc tatttttatt caataattat aaagtgctaa taatcaatat tatggtatta
    11101 tattctgata atgatttgat aagcgaattc ttttgccaga tggtatttct atttccaccc
    11161 cataatttat atctttttca ttaggaagtg gcctctttaa ataaggcgtg tgtgacattt
    11221 ttttggggtt aagcgtctcg tattgaaata cttcgaactg atattcagac atttctgccc
    11281 atgtttgaat tttattgtat cgagagagtg tcgtaaaggc tttaacttgc ttataacatt
    11341 gtttttggaa ttcttgctga tatgttaata tgtcattgta tgaaagattg gttatcctgg
    11401 cctctaaaaa tttaattatt gtcgaagcaa tttccgcttt tgctcgtaaa aagttgtcat
    11461 taatggttaa taatgttgaa aataaacctt cgatttgttt gtcaagatat tgtccatctt
    11521 tatataattt gggggtaggg tgttctttat gtaaaaaata gtgtcaccta aattttcgtc
    11581 agtgaaattt atattaagta gatcttcgaa ataaaacctt gacaaggtta gaacgaaccc
    11641 ggacaggttc tgtcctgtca tattgaaatc aataaagttg gttccggcaa agactttcat
    11701 cctgttttta ttttcactgt gctcaatata taacttcgtc attttttgat acaggctttg
    11761 aatatctttt atattagttg gtaagtcagc tcgttgttca gcaataaaat tagtatctga
    11821 aaacatggtt tggacactga atgttatggg tattccctct tctacgctaa ttttagttat
    11881 gattgccttg tcgggtttag gattagcgac aacaatgatt cgacattcag cacttttgtt
    11941 tttttcatat ctaatgttat taatctgatt aagtattgat tgtgtctgtt gcgtaagttg
    12001 tgtcgaactt atcgtttcaa tgttagtaat aagacttaca agttgagcca caacttttga
    12061 gtagtctgca tttgtatatg tagtgttcgt gtggcacgca ttaatttgtt gaagttggtg
    12121 ttgcgctaaa tcatattgat cagagatata tccttctcca gagaatgtgg aattaatggg
    12181 ttattgttct tcccaattat cgcaaggcca cttcggtgtg tctggcgttt tagcattatc
    12241 ggtatttgtt gtgcagttat aaatatcgcc gtgatatgta tttattatac ctggggtaaa
    12301 gtcaattgac ataaaattcc ttattcatca tttgttttat gctaagatgt ttaaattgga
    12361 taattagaac aggaatggat tttcttctac acacaataaa tataatgcgt tatatcttcc
    12421 tgatataatt acctttaaat tccctttcat ttccacccgg taatatttgt gtataaaaaa
    12481 aggcagtgat agtatcgtaa agggaaacgt taacaactat caaaaagcgc tcattttaaa
    12541 aagttatttt gttctccgat agtgtgtgaa gtaatatttt tgtgatgttt tagatgtgta
    12601 ataatgaacc aggttaaagt gtatttaaaa atgtgttgta ttagcgactg aaatctgcct
    12661 tttaaaaagg aggagggtac tgagacaaag agtcattgct tctccgataa aatgtcagag
    12721 aagcatgatt acgtggttat tgtctgttga tgacttgttg accgagccgt catcattctc
    12781 aatgcattag ttaaatcatt tgtgattttt aaggattatc ctgtctatga ttttctactt
    12841 agaaggatat ggcactaact atgcatctta tagcttactg ctggcatcta tttaaaatag
    12901 caattgagtc aaatagccca tcaagatagc catacctaat ataaccgtca ggaatgcagc
    12961 tatcatcggc attctgaaca ttgatttaag taatatcact tccgtcaggc ttgcgccggc
    13021 actgccgatg attaatgcca ttaatgctcc cagacccatt ccttttgtca tcagaacaga
    13081 tgccagaggg ataactgcct cagcccggat atacagcgga ataccgacaa cggcgcttaa
    13141 tgggatggcg aagggattat ctgctcctgc atgagcggca atccacgctg agggaataaa
    13201 gccataaata aaagatccta ttaatactcc taacaaaaga tatggcagta catctttaaa
    13261 ctgttgcaac gcatctttgg tggctagttt tatgggatta atacttacta cagctttggt
    13321 actgcaacag gtaactacgg ggttctcaat ggctttagcc gctggactac aacaactcac
    13381 ttttatgggc gtatatgtcg ttcccggcga agtttttgct ggagcacaac aatttgctga
    13441 tgagctttta ctggcaatga tatgacgttc aaaccccaga gaatccagga taatactggc
    13501 aagaacggag acgccggctg cgataatcgc gtacaacaag gtgactttcc aaccaaaggt
    13561 catccacatt agtccgacga taatgggatt aagtaatggt gaaacaaata aaaaagtgag
    13621 agtcggacca aacccggctt tcgctgacaa caatccacgt aacatgggta ttgtcgagca
    13681 actacagaac ggggtaacgg ctcccaacag agcagccagg agatagcctc tcccttttct
    13741 ggcccccatc atctgctgga ttttatggtc tggcaccttt tgtcttatca ggctgacacc
    13801 ggcactaatc actataaaca gcaacgaaag ctcgacggcg agaaacacaa acatctctgc
    13861 ggcatcttgc agcatcgcaa gccaactact catacgtaac ctcattggat aaatctatat
    13921 ttccagaata atcgaaatga aattcagatc aagatatttc tggtattatc gaaatatcaa
    13981 ccagacagag gggtgtcata tgcagttgga agaagtcgct aaagcgctga aagagctagg
    14041 tcacccgacc cgtttgttca tatttaaaca tcttgtcaaa gccggggaac aagggttgcc
    14101 ggtcggtgag ttgcaaaagc agcttgggat acctggctcc acactcagcc accatatatc
    14161 agcactggta tcggtaggac tggtcaaaca aaaccgtgag agtcggacgc tgatgtgtgt
    14221 ttcgcaatac gcaatgctgg aagcgataat tgaattctta cgcgaggagt gctgcgtaaa
    14281 tagcaaaatt gcctgatgcg ctacgctcta catatttcat gccatatatt gaatttgcac
    14341 ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa agcgcacttt
    14401 accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc ttcatcaacc
    14461 actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag aatttccagc
    14521 accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat cgggcgatta
    14581 atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa tttatcttcc
    14641 gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg tacggaaatc
    14701 cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc cagataatgg
    14761 ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg cgacgtgccg
    14821 caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta caaagtgaaa
    14881 gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt catgataatc
    14941 cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag tacgtgcagc
    15001 cagagtagcg tagccaggct accaattggg gtaattttcg gtcccaaatc gcagccaatc
    15061 acattggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc atcaatggac
    15121 aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa ggcggtgagg
    15181 aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt gagtacgcca
    15241 gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata catgccgagc
    15301 gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt aatcgcatga
    15361 ccgcgtttag cgacgacaaa taatatcagc gcgcccacag ctgcaatggc gctcaccgga
    15421 atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac aacccagcca
    15481 gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag cgccatatcg
    15541 tagttctgcg gaatatcttt gcgaaaatag agatgtaaca tcaccagcgt ggcaacaatc
    15601 gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag gccaaagaaa
    15661 tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc ggtatcggca
    15721 atgaatccgg ccgccatcac gaacgccagc gtagtgcctt tactgaaccc taaagccagc
    15781 agcatggcga tgacaatcgg tgtcaaaata agcgccgcgc catcattggc aaacagggcg
    15841 gcaacggcag caccgagcag gacaatccag gtaaacagca agcgaccacg accattaccc
    15901 cagcgtgaga cgtgcagcgc cgcccattca aaaaagccgg actcatccag cagcaggctg
    15961 atgataatga cggcgataaa cgcagccgtc gcgttccaga cgatattcca caccaccgga
    16021 atatcacccg gatggaccac gcccgtaact aacgccagta ctgcgccgag cgttgcactc
    16081 cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag gacaaagata
    16141 gcgcctgcca gtaacataat gcctcccgga taaaacacat ctgaaaattc atatgtgttt
    16201 agctaaattt ttaactgcaa atgttcttac tgtccccgga acagttttgt cgagccaggt
    16261 tgcggacaat cgcctgaacc ttttcctgtt cacatcgcca ggcctcatca ataattttcg
    16321 ccgcccatgc tggaatatgc ggtgataagc ggtaatgaac ccacttacct tgcttgcggt
    16381 ccagcaatag cccgctttca cgcagcaatg ccaggtggcg ggagatcttg ggctgcgact
    16441 ggtcgagagc agtgcagaga tcgcagacgc ataactctcc cagttcgctg agcagtaaaa
    16501 cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt aacagaaatg
    16561 acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat aagtcaaaaa
    16621 catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga attttgagtt
    16681 ggctattcat ttgaaaggag gtctgaatca ggacgtaaaa aaaccgactt tgcgtcggtt
    16741 ttttactttc cagccctgag ttggtggctc tggctggagt gagagagttc ttatctaaca
    16801 gctcaatact attagccagt ggctaacatt gcaagaatta gcgatttctt cagctggcgg
    16861 agggggcact tcggtatata tcgcaatgac gatttatata ctttcactgg gtcatcgtca
    16921 tattaagcct tgtccgaatc cggatgtaaa aaaccgactt tgcgtcggtt ttttactttc
    16981 cagccctgag ttggtggcac tggctggagt gagaatgcca ttatctaacg catcaatgct
    17041 aataccatac tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg
    17101 attatccagt tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt
    17161 cctggtcaga agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agaatgccat
    17221 tatctaacaa tagggcatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca
    17281 ctccatcgca tggagaaaac gggtgattga taaagcaatc atcgttcttg gggcgttaat
    17341 tgcgctgctg gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa
    17401 ttaagggcta agagcacact actcttagcc ctttaacatt taacgcattg tcacgaactc
    17461 ttctgccgcc gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcacccat
    17521 cttcagcgcc accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat
    17581 accgacaatc ttctcttccg atccaacgca caccagcttc atgcggcacg gctggcggtg
    17641 agtggtgacg gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc
    17701 gccatactgc tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct
    17761 gaagaccacg gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag
    17821 gcgttcagag agacggcgac ccgctgcaac tgccaccggt gtcagctcca ctgcacccgt
    17881 gttatcgccc accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat
    17941 atagcctttt tcgttagttt taacgccagc ggcttccagg ttgatgttgt cattggcagg
    18001 ctcgcgacca atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc
    18061 cagcgtcagg ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag
    18121 ctgcgggcct tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct
    18181 gcgcagcggc gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac
    18241 gcccgccagc tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc
    18301 aggaagggcg aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg
    18361 gctcggacga ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac
    18421 ctccagcgtt ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt
    18481 accgagcacg ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat
    18541 caacgtttcc cagttgaatt tattgatagt ggtatcaaaa ccataatccg ggccgtacat
    18601 atggatcgct tcacggattt gcgccgcgtg ccacatcact tttttcggca cacagccaac
    18661 atttacgcag gtgccgccca gctctttggc ttcaatcagc gcacatttct ggccgtacat
    18721 agccgcgcgg ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata
    18781 gtgtttagtc atgacaaagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta
    18841 ataggtgcca gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg
    18901 gtgcctgccg gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc
    18961 agtttccacg gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg
    19021 tctggcagta ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg
    19081 tggatcatgc gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca
    19141 gtggcgaaac gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc
    19201 atttcatacg gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg cagtttggcc
    19261 ttaagctgct ggaaaccatc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca
    19321 gaacgcagca acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg
    19381 cgcagtagct ggcgagcaat caatggcgaa ccggggtagt aacgcaactg accgctacgg
    19441 ttgaagtgtt ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc
    19501 cagatacggg cgatgccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac
    19561 tgataacgcc cggcccctgc gtgggtgtcg agatagagaa acggtttatc tttctctttc
    19621 agcgactcga tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg
    19681 tgaaagctgt ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc
    19741 gtattcgggg cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga
    19801 aagcgtaacg tttctcattg aaattcacta cacttaaccc catgctacac acattatgta
    19861 aagcgcctgt tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg
    19921 ttactgactc cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca
    19981 gccgtgacta aggcgctgaa cgactgccgc gaaaatgtgg agcgcgtagt agcgcaagga
    20041 gcaccgtaca cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgctgggg
    20101 cgtatcttct ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa
    20161 gcgtacgaac aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa
    20221 gggctgtata aagcgtatcg cgacctgcgc gatggcgatc attacgccac gctgaacacg
    20281 gctcagaaaa aagcggttga taacgcactg cgcgacttcg aactctctgg catcggtctg
    20341 ccaatagaga aacaacaacg ctacggtgag attgccactc gtctttccga gctgggcaac
    20401 cagtacagca acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa
    20461 gcggagctgg cggggatgcc tgaaagcgcg ctggctgcgg caaaagccca ggccgaagcg
    20521 aaagagctgg aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc
    20581 tactgcgaca accaggcctt gcgtgaagag atgtatcgcg cttacagcac ccgcgcctcc
    20641 gatcaaggcc cgaacgccgg taaatgggat aacagcaagg tgatggaaga gatcctcgct
    20701 ctgcgtcacg aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc
    20761 actaaaatgg cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc
    20821 gcgcgtccgc aaggcgaaaa agagctggca caactgcgcg ccttcgccaa agcggaattt
    20881 ggcgtcgatg agttgcagcc gtgggatatc gcgtactaca gcgaaaaaca aaaacagcac
    20941 ctctacagca tcagcgacga gcagctgcgt ccgtacttcc cagaaaacaa agcggttaac
    21001 ggcctgtttg aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagatgtt
    21061 gatgtctggc atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc
    21121 ggcagcttct acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat
    21181 gactgcgtag gccagatgcg taaagctgat ggttcgctgc aaaaaccggt cgcgtatttg
    21241 acttgtaact tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgaggaagtg
    21301 atcaccctgt tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc
    21361 gctggtgttt ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt
    21421 atggaaaact ggtgctggga gccggaggcg ctggcgttta tctccggtca ctatgaaacc
    21481 ggcgaaccgc tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg
    21541 gcgctgttta ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag
    21601 ttccgcccgg atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt
    21661 gccgtggtgc catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc
    21721 ggtggttatg cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct
    21781 ttctcgcgct ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac
    21841 aacattctga gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt
    21901 cgtgaaccgc agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt
    21961 gaaaatctgc ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc
    22021 ccgctggggg ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca
    22081 tctggaattg cgcaagcgtg atgagccaaa acttggcggc atatttgttg attttgttgg
    22141 cggagcgatg gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc
    22201 ggtgggcatt aaaggcgatt atttgccgga tgtggtggac gccaccgcag ggctggggcg
    22261 cgatgccttt gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt
    22321 cgttgccgca ttgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg
    22381 ctggttgcag gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat
    22441 taccccacgc ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc
    22501 gctggtgaaa aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga
    22561 tggattgctg gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga
    22621 ctacgcgcca ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg
    22681 ctttgatatc tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc
    22741 ctgctttctg caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg
    22801 gcaccatcaa cagcatcact actgcaacgc ccagcgtgac cagaccaatc ttaccaaaca
    22861 cgttggtata gacgggcaac gtctcaagcg gatcggtaat gttgtccggc accgcggtaa
    22921 atgttgccac atagccgccc agcaagaacg cggcagcctg cgtcaggaac cacatcccga
    22981 gaataaagcc catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc
    23041 taataaacag ttcacctaag ctctggaata agtacaccag cacgataaac catggcgatg
    23101 tcagcccttg tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg
    23161 agcacataaa catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac
    23221 ccagatgcgt gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa
    23281 gcgcctggaa gctgaccggg ttgatggaaa aaccgagaat ttcatgatgc acgttgttga
    23341 tggcaaagaa gttcagcgat gttggcatct gggcgtagag aatgtaaaac accaccgctt
    23401 cgagcatcag gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg
    23461 cctgacgaaa gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga
    23521 cttctacgtt gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata
    23581 cgtacaacag tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca
    23641 ccattccacg acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt
    23701 tgtaggtgac tgaataaccg aatctatcag cgatcacagg ggccagcgat aacgctatca
    23761 acgagccgat attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt
    23821 tcggcggata gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga
    23881 cagcgatagt ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca
    23941 tgaagtagcc aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca
    24001 ggtggtcgcc gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac
    24061 caaaagtgac aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca
    24121 gtacgccctg cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga
    24181 aaaatgggcg aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc
    24241 ttccaatacc atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat
    24301 ctgtcagaga ttcttaatat tgatgcggtg aatagattaa tacactattg caacagaaaa
    24361 taaccagctg atcacactaa tgatggaaaa tatgatggtg ttaaatgttg tattggcagg
    24421 agattttgat tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa
    24481 caaggcgcgc caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtggag
    24541 ggatgattat tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt
    24601 gatcagctga cgtgcggaag acatcagttt gctccagaag tcctggtggt gaccacaaac
    24661 caccagatcc atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct
    24721 gccgctcagg gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg
    24781 atgtgtctct tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt
    24841 gtataggtca gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg
    24901 agccatagag actgctttct ctaccagaac tttgctttcc ggggagagat cgactgcgat
    24961 gagaatgtgt ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag
    25021 ctagcgtttc ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac
    25081 ccagccgtaa agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt
    25141 caatccgcct tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg
    25201 ttaaccttct ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg
    25261 ttggtcacca caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg
    25321 gagcggtagc cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc
    25381 accgtcgcat tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc
    25441 tcatcactac gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat
    25501 gttgatggag ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt
    25561 tggtatatct atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt
    25621 gagcgggtgc gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc
    25681 ctgattgcat tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg
    25741 ggccagtaaa ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg
    25801 cttatcagga tctacataat ctctgcaatc tattgaattt gcatgttttt gtaggccgga
    25861 taaggcgttc acgccgcatc cggcatgaac aaagcgcact atgtcacaat ctgaagcggg
    25921 ccagctgacc cgctcgtttt ggtgtgtacg attacaggaa ctgcaaggag agccagtaca
    25981 gtccgccgga aagcagtacc gcagccggaa gggtaaacac ccaggccatc agaatactgg
    26041 ttacggtttt acgctgtaag ccgccgccat ctaccaccat cgtccccgcg acagaagagg
    26101 agagtacgtg agtagtggaa accggcatcc cggtataact cgccaggccg atagacactg
    26161 ccgccgtcat ctgggccgac atcccctgag cgtaggtcat gcctttctta ccgattttct
    26221 caccgatagt cgttgccaca cggcgccagc caatcatcgt accgatacct aacgccagcg
    26281 cgaccgccat gatgatccac accggtgcat actcgatggt gctaagcatg tcggacttca
    26341 gtttcttcaa caggcgctga tcgtcagcac tcacaccagg catcttcacc actttgtcga
    26401 tagtgtcaga aacgcacagc ataatgcggc gcatctggct acgttgatca agcgacagct
    26461 tgtcgtagct ttccacatcg gtagtcaaca tgcctttcag gcggttgagc gcgttaatgg
    26521 tattcgacgg atggcaatgg aactccgcgg gttgcgttgc gccagcttcc ggagccggta
    26581 ctaactgatc agcaccggta gcctgtttga gcagcgcagg atgctgctca aagtaagctt
    26641 cgacgttgtt gatggcatca cgggtacggg tgatttcgta gccagtggca ttcatgttca
    26701 ccacgaagcc tgctggtgcg acgccaatca ataccaacat aaccagacca atgcctttct
    26761 gaccatcgtt cgcgccgtgc gaaaacgcca cgccgatagc ggaaaggatc agcgcaatac
    26821 gcgtccagaa cggcggcttt ttcttgccgt ctttcttttc acgctccgct ggggtcaggt
    26881 ggatacgggc gcgtttcttg gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc
    26941 cagcaaacac caggccgaca ataggggaaa cgatcagaga accgaaaata cttaatactt
    27001 tcgggatatt gagtgcatcc accactgacg tcccggtcat caacgcattg gttaaaccaa
    27061 tcccgatgat cgcgccaatc agcgtatgag agctggatgc aggtaaacca aagtaccagg
    27121 tacccaggtt ccagataatc gccgccagca acatagagaa caccatggca aggccatgag
    27181 acgatcccat attaagcagc agatccgtcg gcagcatatg cacaatggca taggcaacac
    27241 tcagaccacc cagcaaaaca cccaaaaagt tgaacaccgc tgccataacc acggcgagct
    27301 gagaacgcat cgcgcgggta tagataacgg ttgccacggc gttggctgtg tcatggaaac
    27361 cattgatggc ttcgtagaac agcacaaaag ccagtgcaag caataataac agcccggtat
    27421 gcaaatccag gccagcaaac aaatgtagca taggacgtta cgccattttg aggacatgaa
    27481 cgcggcgcat tatcagtgac tttcgcggcg cgggcaaagt gaaatataga ctttttttga
    27541 tttgcctcct gtatggattt cactcaaaaa ataattatct tatataattc aggcaaatac
    27601 ttccttttag taatattgat gctggtgcga ccactgagga atctttacaa ttcacgcccg
    27661 ttttttctaa gaggagcgca acgtggaaag gtttgatgcc attattatag gcgctggtgc
    27721 ggcgggtatg ttctgttctg cgctggcagg tcaggcagga cgccgggttc tgctgatcga
    27781 taatggtaaa aaaccagggc gcaaaatcct tatgtctggc ggtgggcgct gcaactttac
    27841 caacctttat gtcgaaccag gcgcttatct gagccagaat ccgcattttt gtaagtctgc
    27901 actcgcgcgt tttacccagt gggatttcat tgatctggtc aataaacacg gcatcgcctg
    27961 gcacgagaaa acgttaggac aactcttctg cgatgactcc gcgcagcaga ttgtcgacat
    28021 gctggtggat gagtgcgaga agggcaatgt aaccttcaga ttgcgtagcg aagtgctgag
    28081 tgtggcgaag gatgaaacag gcttcacgct tgaactgaac ggcatgactg tcggttgcga
    28141 aaagctggtc atcgcgaccg gtgggctgtc aatgccgggg ctgggcgcgt cgccgtttgg
    28201 ttataagatt gccgaacaat ttggcctcaa cgtgctgccg acccgcgcgg gtctggtgcc
    28261 attcactctg cataaaccgt tgctcgaaga gttacaggtg ctggcgggcg tggcggtgcc
    28321 ttccgtgatt accgctgaaa acggcatcgt tttccgtgag aacttactct tcacccatcg
    28381 cggcttgtct ggaccggcgg tgttgcagat ttccagctac tggcaaccgg gtgaatttgt
    28441 cagtatcaat ctgctaccgg atgtggatct cgaaaccttc ctgaatgagc agcgtaacgc
    28501 acatccgaat cagagcctga aaaacacact ggcggttcat ctaccgaagc ggttggttga
    28561 acgcttacag caactcgggc aaatcccgga tgtttcgcta aaacagctca acgtgcgtga
    28621 ccaacaggca ctgattagca cattgaccga ctggcgcgta caacccaacg gcactgaagg
    28681 ctatcgcact gccgaagtga cgctcggcgg cgtggacacc aacgaactct cttcacggac
    28741 gatggaagcg cgcaaagtgc ctgggctgta cttcatcggc gaagtgatgg acgttaccgg
    28801 ctggctgggg ggctataact tccagtgggc gtggtcgagt gcgtgggctt gtgcgcagga
    28861 tttgattgca gcgaagtcgt cctgacctgt tacctaaaga tgaaaattat tagagccgca
    28921 ttaaaaatga gctaattttg atagtggcta tcttgtgatt attttctaat gagcccgtga
    28981 actgaaaccc tccaggctta atataaggtg gaaggaaagg tgattgaaaa ctcacccagt
    29041 ggccagatct ttataaaata tgaacaggtt gaaaaaaaca gtaactttgt tgttttttta
    29101 tccttaacca ctatgcatta atgctgcgtt atttcatgat gcctaagaaa aaccagcgtt
    29161 acgcaaatgg tcaacgctgg ttttatccgg tacgttgcaa ttatttttta gcagaacctg
    29221 cttctaatat tgaaacgatt gagaacaacg taaagcatac cgctcctaaa ccgaccaaaa
    29281 cccgcgaagg aataaaatat cccatgtcgg tctgcgccat ttccgcaaga aacgatgcca
    29341 ggaaaaggca aaacaggcag gtgaagacgg gaatcatcgg tattctgttg gctaacgaac
    29401 aggtacgtct ccataccagt gccagtagcc agacttttga gaatatgctg taacagatca
    29461 ttccgaggca aataagaata atgccgggag ccagtcgggc gcttgcatcg gaactgacta
    29521 agacgtatat cccctgcagt accgtgattg agccgagaaa aataacccaa taacaccaca
    29581 gccagtgttc tttagttgaa aacgtattac gtgtttgatg gacaattgtg gcaacaaggc
    29641 caattagaca ggcacagatt gcggttaacc caagcaatac gtgacccgcg acataatgcg
    29701 gagttatgtc agcactacgt aacagcgtaa tagaccaaat gaaaccgagc agggtgagta
    29761 aaacgggaac ggcaatgagg caattaccga ttaatgaaga ataagcctgt accggcgttc
    29821 catcgctctt gctccccgct gcatttttgg gaatgagcag aaagtgaccg gatgacgctg
    29881 ccaccgtcga tacacaggcg gcaatcatac caacgccgaa aataacatgg ccggcgacaa
    29941 actcgtctgc catcacatca ttgcctgcta acagtgccca accccatatc atggtgataa
    30001 ttgaccccgc atagccaata atggggaaca atgtattgta aaacgtatta acgccgcgcg
    30061 tgagctgcga aatgataata aatgcggtag tgaataatgc caggcatatg gctgccagag
    30121 aaatcagcac atgacccgca acgaaatact cactttgccc agaactttcc agcacaaagc
    30181 cacctaacgc gatacaaatt aagcccataa ttaaagggat taatttgaaa agccacccga
    30241 tatatatgtt cactttaaac tcctcaggta tccctgctaa tattgaaatg tgcattaatg
    30301 ataatttcag cctgttaata taatgacgag atatcattaa agacaaatgc atagcataac
    30361 tatagcggga tttactttgc agagcgctac ttttcagcgt tcaaaaggcc atggtaaata
    30421 aatatttatc ctaagattaa ttcttattct gttgtttttt acaattatct tccagggagg
    30481 gattcttttt cattatttgg taaaagaccg gatggtatcg gtgcttaaaa tcagcgaaca
    30541 tataagtatt attgttttat atcgtcgaaa atatgtcttt cagacatgta ttatttattt
    30601 tgttactaac aagtcaaaaa tatcgcaggt tatttatgaa agcaattgac aatatattta
    30661 agtacgtccc ttcaaaccat aaagataagc attcagataa agttaataat catcaacacc
    30721 atagcaaggt tgataaaaca catcgttcaa agatagttga aattgataaa ctggataatg
    30781 attcgcaaat agataacgat tttggcatgc atatcatcta tttcctgcaa catggtcact
    30841 ggagggtaaa tgatcgtagt catcagatgg agaaagtatg gttttacaat agtgaacctt
    30901 ctatagatat tca
//
LOCUS       DAZFJF010000049        30738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFJF010000049 DAZFJF010000000
VERSION     DAZFJF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.049"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..166
                     /gene="xisR"
                     /locus_tag="ACHKAU_002402"
     CDS             <1..166
                     /gene="xisR"
                     /locus_tag="ACHKAU_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5531455.1"
                     /translation="LKKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQ
                     ASARPRRHLT"
     gene            201..1481
                     /locus_tag="ACHKAU_002403"
     CDS             201..1481
                     /locus_tag="ACHKAU_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5531456.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSKFAPTMPHNEAMKN"
     gene            complement(1669..2688)
                     /gene="rspB"
                     /locus_tag="ACHKAU_002404"
     CDS             complement(1669..2688)
                     /gene="rspB"
                     /locus_tag="ACHKAU_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO5531457.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2700..3914)
                     /gene="rspA"
                     /locus_tag="ACHKAU_002405"
     CDS             complement(2700..3914)
                     /gene="rspA"
                     /locus_tag="ACHKAU_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO5531458.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4120..4446)
                     /gene="ynfA"
                     /locus_tag="ACHKAU_002406"
     CDS             complement(4120..4446)
                     /gene="ynfA"
                     /locus_tag="ACHKAU_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO5531459.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4581..4922
                     /gene="ynfB"
                     /locus_tag="ACHKAU_002407"
     CDS             4581..4922
                     /gene="ynfB"
                     /locus_tag="ACHKAU_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO5531460.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4957..5517
                     /gene="speG"
                     /locus_tag="ACHKAU_002408"
     CDS             4957..5517
                     /gene="speG"
                     /locus_tag="ACHKAU_002408"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO5531461.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5520..6230)
                     /gene="ynfC"
                     /locus_tag="ACHKAU_002409"
     CDS             complement(5520..6230)
                     /gene="ynfC"
                     /locus_tag="ACHKAU_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO5531462.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6338..6643
                     /gene="ynfD"
                     /locus_tag="ACHKAU_002410"
     CDS             6338..6643
                     /gene="ynfD"
                     /locus_tag="ACHKAU_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO5531463.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            6842..9267
                     /gene="ynfE"
                     /locus_tag="ACHKAU_002411"
                     /pseudo
     CDS             6842..9267
                     /gene="ynfE"
                     /locus_tag="ACHKAU_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            9328..11751
                     /gene="ynfF"
                     /locus_tag="ACHKAU_002412"
     CDS             9328..11751
                     /gene="ynfF"
                     /locus_tag="ACHKAU_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO5531464.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWVKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            11762..12379
                     /gene="ynfG"
                     /locus_tag="ACHKAU_002413"
     CDS             11762..12379
                     /gene="ynfG"
                     /locus_tag="ACHKAU_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO5531465.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12381..13235
                     /gene="ynfH"
                     /locus_tag="ACHKAU_002414"
     CDS             12381..13235
                     /gene="ynfH"
                     /locus_tag="ACHKAU_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO5531466.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13278..13892
                     /gene="dmsD"
                     /locus_tag="ACHKAU_002415"
     CDS             13278..13892
                     /gene="dmsD"
                     /locus_tag="ACHKAU_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO5531467.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14051..15343
                     /gene="clcB"
                     /locus_tag="ACHKAU_002416"
     CDS             14051..15343
                     /gene="clcB"
                     /locus_tag="ACHKAU_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO5531468.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15296..15991)
                     /gene="bioD"
                     /locus_tag="ACHKAU_002417"
     CDS             complement(15296..15991)
                     /gene="bioD"
                     /locus_tag="ACHKAU_002417"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5531469.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16116..17336)
                     /gene="mlc"
                     /locus_tag="ACHKAU_002418"
                     /gene_synonym="dgsA"
     CDS             complement(16116..17336)
                     /gene="mlc"
                     /locus_tag="ACHKAU_002418"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO5531470.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17471..18364)
                     /gene="ynfL"
                     /locus_tag="ACHKAU_002419"
     CDS             complement(17471..18364)
                     /gene="ynfL"
                     /locus_tag="ACHKAU_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5531471.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            18471..19724
                     /gene="ynfM"
                     /locus_tag="ACHKAU_002420"
     CDS             18471..19724
                     /gene="ynfM"
                     /locus_tag="ACHKAU_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5531472.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(19773..19892)
                     /locus_tag="ACHKAU_002421"
     CDS             complement(19773..19892)
                     /locus_tag="ACHKAU_002421"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO5531473.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            20148..20456
                     /gene="asr"
                     /locus_tag="ACHKAU_002422"
     CDS             20148..20456
                     /gene="asr"
                     /locus_tag="ACHKAU_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO5531474.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            20549..20632
                     /locus_tag="ACHKAU_002423"
     CDS             20549..20632
                     /locus_tag="ACHKAU_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531475.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            20732..21553
                     /gene="ydgD"
                     /locus_tag="ACHKAU_002424"
     CDS             20732..21553
                     /gene="ydgD"
                     /locus_tag="ACHKAU_002424"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO5531476.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(21592..21921)
                     /gene="mdtI"
                     /locus_tag="ACHKAU_002425"
     CDS             complement(21592..21921)
                     /gene="mdtI"
                     /locus_tag="ACHKAU_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO5531477.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(21908..22273)
                     /gene="mdtJ"
                     /locus_tag="ACHKAU_002426"
     CDS             complement(21908..22273)
                     /gene="mdtJ"
                     /locus_tag="ACHKAU_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO5531478.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            22685..22828
                     /locus_tag="ACHKAU_002427"
                     /pseudo
     CDS             22685..22828
                     /locus_tag="ACHKAU_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            complement(23587..23868)
                     /locus_tag="ACHKAU_002428"
     CDS             complement(23587..23868)
                     /locus_tag="ACHKAU_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531479.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRALELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(23882..25543)
                     /locus_tag="ACHKAU_002429"
     CDS             complement(23882..25543)
                     /locus_tag="ACHKAU_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5531480.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLESPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVMHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(25527..25883)
                     /locus_tag="ACHKAU_002430"
     CDS             complement(25527..25883)
                     /locus_tag="ACHKAU_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO5531481.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(26006..26188)
                     /locus_tag="ACHKAU_002431"
     CDS             complement(26006..26188)
                     /locus_tag="ACHKAU_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531482.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(26172..26612)
                     /locus_tag="ACHKAU_002432"
     CDS             complement(26172..26612)
                     /locus_tag="ACHKAU_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5531483.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(26612..26908)
                     /locus_tag="ACHKAU_002433"
     CDS             complement(26612..26908)
                     /locus_tag="ACHKAU_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5531484.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQLVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(26905..27243)
                     /locus_tag="ACHKAU_002434"
     CDS             complement(26905..27243)
                     /locus_tag="ACHKAU_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5531485.1"
                     /translation="MNIGRLRDRVTIQTLKQTRDITGEILETWVDGHTLWASVNMISS
                     KEAISSGAELATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(27240..28451)
                     /locus_tag="ACHKAU_002435"
     CDS             complement(27240..28451)
                     /locus_tag="ACHKAU_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5531486.1"
                     /translation="MWPFRRKKEQRSMTLDGFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(28453..29025)
                     /locus_tag="ACHKAU_002436"
     CDS             complement(28453..29025)
                     /locus_tag="ACHKAU_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5531487.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDNLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDDTGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(29065..30222)
                     /locus_tag="ACHKAU_002437"
     CDS             complement(29065..30222)
                     /locus_tag="ACHKAU_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5531488.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGATFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFAQKYLGGGVVDSNAIKILELPQDDD"
     gene            complement(30513..30737)
                     /locus_tag="ACHKAU_002438"
     CDS             complement(30513..30737)
                     /locus_tag="ACHKAU_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531489.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
BASE COUNT         7602 a         7964 c         7404 g         7768 t
ORIGIN      
        1 gttaaaaaag gcccgggaaa aatcgtttat ggaaggaagg gaatataagc atgtcgctca
       61 tgacggtatg ccatgggata acagtccatg cttttataac ctggaagaaa ttgatcgctg
      121 gattgagcgc caggcatcag cgagaccaag acgtcatctt acttgactaa aagccacact
      181 aactaatgag agaagttgaa atgaaatatc cgacaggcgt ggaaaaccat ggagggaaat
      241 tacgtatctg gtttgtttat aaaggcgtaa gagtcaggga aaatctgggg gttcctgaca
      301 catcaaaaaa caggcgcgtt gcaggtgaac tacgctcctc tgtttgttac gcaataaaaa
      361 ctggtgtttt cgactatgca aaacagtttc cctcctcacg caatctggaa aaatttggtg
      421 aggcccgaca agatttaacc ataaaagaac tggctgaaaa atttttggca ctgaaagaaa
      481 ctgaagtcgc caaaacatca ctcaacacat accgtgccgt catcaaaaat atcctgagca
      541 taatcggtga aaaaaatctt gcctcatcga ttaataaaga aaaattactg gaggttcgta
      601 aagagctact gactggatac cagatcccca aaagtaacta tattgttaca caaccaggga
      661 gatcggctgt aactgtaaat aattacatga caaatcttaa cgccgtgttc cagtttggtg
      721 ttgataacgg ttacctggca gataatccgt ttaaggggat ctcgccatta aaggaatcaa
      781 gaaccattcc ggatcctctt tcgcgggagg aatttatccg tcttatcgat gcgtgcagaa
      841 atcagcaagc aaaaaattta tggtgtgttt ctgtttatac tggcgttcgc cctggtgagc
      901 tgtgtgcact tggatgggag gacatagatc tgaaaaatgg aacaatgatg atcaggagaa
      961 atttagcaaa agaccgtttc acggtaccaa aaacacaggc gggaaccaat cgggtcattc
     1021 atcttattaa gccagcaatc gacgctctcc ggagtcagat gacattaacg agactgagca
     1081 aagagcatat cattgatgtt cacctcagag agtatggcag aacagaaaaa caaaaatgca
     1141 cctttgtttt tcaacctgaa gtgtcagcga gagtaaaaaa ttatggtgac cattttaccg
     1201 ttgactcaat aaggcagatg tgggacgcag cgataaaacg tgccggactc cgccatcgaa
     1261 aatcatatca gtcgagacat acttatgcct gctggtcgct gacagctggt gctaacccgg
     1321 catttatagc aaaccagatg ggccatgcag atgcgcaaat ggtatttcag gtatacggaa
     1381 aatggatgtc tgaaaacaat aatgcacagg tagctttgtt aaatacacag ttaagcaagt
     1441 ttgccccaac catgccccat aacgaagcaa tgaaaaatta atttaatatt tatcaaatag
     1501 ttaacacgca tgactcttga aatccataaa ttcaagcgca gtgcccagcc atcccgatac
     1561 tgctgctttc accaaatcct tagtgcttct ttcatgtttt tctattgtca taatggttat
     1621 ctctaaaaaa gaggtaagat gcgtactact tactcgccgt tattggtatt attcagaaaa
     1681 agtgagcaag actttgcagc aatgtttttg atcctgttca aataaactaa tggcatcagc
     1741 aacatgctgg aaatcaaacg tatgggtaat taatttttct ggtttaatta accctttact
     1801 taaccagtcg ataacaaccg gaaatttatt tgcatttaag cgtgaagaga aaatagagag
     1861 ttcttttccg gtaattcctt gctgaatcac ttcagacggt tcactggaga agcccatcaa
     1921 tacaatacgt gccgctggag aagccagcgt tacggcctct ttcaggatag aaggatgaca
     1981 agccgcatcg ataattaatg tcggcttgat gcctttttca gcgaaaatct cgccaagcgg
     2041 tgtctggctg ttattaatcg cccagtctgc cccgctctct ttcgcttttt ccagtcgttc
     2101 atcaatgcga tcggcaacaa tcacattttt aacgttatag acgcctttta atacctgaac
     2161 gatcgtcagg ccgattggac cggcaccata aaccagaacg gtatcatttt cagtcggttg
     2221 accatgaccg gtaacgttag ccgcaatggt aaaaggttcg atcatcaccg catattgatc
     2281 ggccactgct tcaggaattt tccacgcatt ttttgccgga accacggcat attcactgaa
     2341 accaccgtca gcgtgcacac ctaatacagc aagtgtcgta caaacgttcg gcttacctat
     2401 agagcacgga tagcaatgcc cacagctgac caccggatcg acagcaacgc gctcaccgac
     2461 tctggcgctt tccacacctt cacccaccgc atcaatgacg ccaaagaatt catgaccaat
     2521 gacgcgcgga tatttcgcaa aaggattatg cccacggtaa atatggctat ctgaaccaca
     2581 aattccggca agtttcactt ttactcgtac ttcacccgct gacggggtgg gtatttcacg
     2641 ttcgataatc gacagttgat tcggtttttc aattaaaatg cttttcatta ccttactcct
     2701 taccagttcc acagcgtgcc atcttccaga cgtgcgactg gcagataagc aggttcatag
     2761 ggatatttcg ccgccagctt ttcatcgaat tcgatgccaa gacccggttt gtctcccgga
     2821 tgcatatagc cgttatcgaa agtccagttg tgcgggaaga cttcgagcat ttgttcggaa
     2881 taacccatgt actcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca
     2941 gccatgcaga ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc
     3001 gaagcaaaat cggcaatccg gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg
     3061 cggatataat cgatgagttg ctcttcaatc agttgtttgc agtcccagat gctgttgaag
     3121 acttcaccca ctgcgatggg tgtgacggta tgttggcgaa tgagacggaa gcattcctgg
     3181 ttttccgcag gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca
     3241 aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta
     3301 aaaccaaact tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc
     3361 gtcgaccaca gctgctcttc cggccactgt cctttggttg cgggttcata agccagacct
     3421 ttacctttcg acatgccgta ggtggttttc ataccaggga ttccgcactg cacgcggatg
     3481 gctttgaatc ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga
     3541 ccggtggtat ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa
     3601 agcggcatgt tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg
     3661 gccgacatcg taaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata
     3721 tcttcgatac ggtgcgcatc gcgaccaata agctgcggac aaaggtgatc ctgcaaataa
     3781 gaggccacgg aaagctcacg tccattgagg gtggcatccc caaggcccgt aataccgtcc
     3841 tcagtggtga tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc
     3901 tttacgatct tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa
     3961 ctgagctact accatacaag cataaagatc gaaaaaagcc ggagtgatca caaaaaaagg
     4021 cgtatatttg cgctgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga
     4081 cacttcctct tatcttattg ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc
     4141 aacaatgatc aacatgccgc aaagcgcaat caacgcaccc gtccagtcat aaagagtcag
     4201 tttcacgcca tccacaacgc gcagccacat caacgccgtg cagacataaa cgccaccata
     4261 agccgcgtaa acacgcccac tcgccgctgg atgcaacgtt aacaaccaga caaacagcgc
     4321 cagtgaaatc cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca
     4381 gggcagaaag catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt
     4441 aatcatcttt gtctcttatt gacatcatgt atagttatag ggcgacataa tatcatcaat
     4501 ataaacaccc tcctggtacg ttttgcgtcc gcagtggatg gtgtagaatc acctttaatc
     4561 attcatacag ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc ctgctcgcta
     4621 ttctgctgcc ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg
     4681 tgattgagtc tggcgacagt gcacaaagcc gccagcacgc cgctatggaa aaagagcaat
     4741 ggaatgacac gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata
     4801 aagccgacgc cgcttttgat aaccgcgata aatgtgagca aagcgccaac atcaatgcct
     4861 actgggagcc caatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct
     4921 aacctgttat tgatttaagg aatgtaagga cacgttatgc caagcgccca cagtgttaag
     4981 ctacgcccgc tggagcgtga agatttacgc tatgaacatc aactcgacaa caacgccagt
     5041 gtgatgcgtt actggtttga ggaaccctac gaagcctttg ttgaactctc tgatctgtat
     5101 gataagcata ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa
     5161 gccggtctgg tggagctggt ggaaattaac catgttcatc gccgcgcaga atttcagata
     5221 attatctccc cggagtatca ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg
     5281 gactatggct ttaccgttct caatctctat aagctgtatc tgatcgttga taaagagaat
     5341 gaaaaagcga ttcacattta ccgcaagctt ggcttttcgg ttgaaggtga attgatgcac
     5401 gagttcttta ttaatggtca atatcgtaat gccattcgca tgtgtatatt ccagcatcag
     5461 tatctggcag agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat
     5521 taataataat cgatcgtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct
     5581 tcatcaacaa tgattagctg acagtccacc ggattagcgt gactgtcata ttcacagctc
     5641 tgttttacat taccaacccg ttgattattc agtaaagtaa ccgctgtgta atctaatttt
     5701 ttgatcggat ccgttgatgg cgtggcgctg acagataatg ttttgtcgtt acttttcgtg
     5761 gttttaccca gcggataacc ctgatcatca tagcgatatt ccatctgcat ttgtttgctg
     5821 ctggctttaa tcacgaagcc attatcatcg gtttcccaac tcaccccggc agaaggtaat
     5881 tctgctagct ggcattttcc ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg
     5941 taataattgg cgtccagtac cagagcgacc acggtattat tttccagatc cagtaattcg
     6001 agtgaatcaa aacagccttc ttccgacaaa gtcccagaaa cacgtttcgt cacttcacct
     6061 tgctcatcca ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg
     6121 aattcattag agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca
     6181 catcctgtga ggaatatcgc gagtaagcat ggtagtaatt tgtatttcac aacagtcacc
     6241 agagagtaga gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct
     6301 atgcttaaag aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc
     6361 cgcgcttctg ctcgccctcg cctcacccgt ggtactcgcc gcgccgggat cctgtgagcg
     6421 catacagagc gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac
     6481 gttaagtatt gtacccaatg accaggttga tcagcctgat tcccaggtcg tcggccattg
     6541 cgctaatgat acgcataaaa ttctctatac ccgcaccacc agcggtaacg tctctgctcc
     6601 cgcgcagtct acccaggatg gcgcgcctgc cgaaccgcag taatacatta tcgactgaac
     6661 gccggatacg acacatccgg cgatttgaac atacaacata atcccacctt attactcata
     6721 cccttctatt gatatggatt aataattctt aacccaaaat gggtagactc cctctattgt
     6781 tagcgcgcta aatattcaat atataaacat ttatataacg ataaagaaca gggagtgagt
     6841 tatgtccaaa aatgaccgaa tggtgggcat cagccgcaga acactcgtta aatcaaccgc
     6901 gataggttct ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc
     6961 agcggcaacg gtacaacagg ccagcgaaaa agtgatctgg ggtgcctgtt ccgtcaactg
     7021 tggtagccgc tgtgcacttc gtctacatgt taaagataat gaagtgacct gggtggaaac
     7081 tgacaatacc ggcagcgatg agtacggcaa ccatcaggta cgcgcctgtt tgcgcggtcg
     7141 ctccatccgc cggcgtatta atcatcccga tcgcttgaat tacccaatga aacgcgtggg
     7201 tacacgcggt gaaggcaaat tcgaacggat tagctgggat gaagccctgg atactatcgc
     7261 cagtagcctg aaaaaaaccg tcgaacaata tggcaatgag gctgtatata ttcagtactc
     7321 ttcggggatt gttggcggca atatgacccg ctcttcgcca tcagcctcgg cggtcaaacg
     7381 cctgatgaac tgctacggcg gttcactcaa tcagtatggc tcctacagca ccgcgcaaat
     7441 ttcctgtgcc atgccctaca cctacggcag taatgatggc aacagcacca cggatattga
     7501 aaacagcaag ctggtcgtga tgtttggtaa caacccggca gaaacccgca tgagcggcgg
     7561 tggcatcact tatcttcttg aaaaagcgcg cgagaaatcg aacgccaaaa tgattgttat
     7621 cgatccgcga tataccgata cggctgctgg tcgtgaagat gaatggctcc ctattcgccc
     7681 gggcaccgat gccgcgctgg ttgcgggtat tgcctgggta ttgattaacg aaaatctcgt
     7741 tgatcaacct tttctcgata aatactgcgt cggctatgac gaaaaaacct tacccgcaga
     7801 tgcacccaaa aatggtcact ataaagccta tattcttggt gaaggtgatg ataacacagc
     7861 gaaaacgccg cagtgggctt cgcaaattac cggtatcccg gtagaccgta tcatcaaact
     7921 ggcgcgtgaa attggcacag caaaacccgc ttatatctgc cagggctggg ggccgcaacg
     7981 ccaggcaaac ggcgaactga ctgcacgcgc tattgctatg ttacctattt tgacgggcaa
     8041 tgtcggcatc agcggcggaa atagtggcgc gcgtgaatcg acctatacca ttaccataga
     8101 acgcctgccg gtgctggata atccggtcaa aacgtcaatc tcctgcttca gctggacaga
     8161 tgctatcgat catggtccgc aaatgacggc aatccgcgac ggcgtccgcg gcaaagataa
     8221 actggatgtg cccattaagt tcatctggaa ctacgcggga aataccctcg ttaatcagca
     8281 ttctgacatc aacaaaacgc atgaaattct gcaggacgaa tcgaaatgcg aaatgattgt
     8341 gatcatcgaa aactttatga cctcatcggc aaaatatgcc gacattctgc tgccagacct
     8401 gatgaccgtt gagcaggaag atattattcc taacgactac gccggtaaca tgggatatct
     8461 cattttcctc cagcctgtca ccagcgaaaa attcgaacgc aaaccgattt actggatcct
     8521 gagtgaagtc gcgaaacgtc ttggacctga cgtctatcaa aagttcacag aaggtcgcac
     8581 gcaggaacaa tggttacaac atctgtacgc caaaatgctt gccaaagatc cggcgttacc
     8641 gtcttacgac gaactgaaaa aatgggtatc tataagcgta aagatcccaa tggccatttt
     8701 gtcgcctaca aagcatttcg tgacgacccc gaggcaaatc cacttaaaac gccttccggt
     8761 aagattgaaa tttattccag caggctggcg gaaattgccc gtacctggga actggaaaaa
     8821 gatgaagtga taagcccatt gccagtttat gcctcaacct ttgaaggctg gaactcccct
     8881 gaacgtagaa ccttcccact gcaactgttt ggtttccatt acaaatcccg tactcactcg
     8941 acctacggca atattgatct cctgaaggct gcctgccgtc aggaggtgtg gatcaaccct
     9001 atagatgcgc agaaacgtgg gattgccaac ggcgatatgg tgcgggtgtt taaccatcgt
     9061 ggcgaagttc ggctaccagc caaagtaaca ccacgtattc tccctggagt tagcgctatg
     9121 ggccagggag cctggcacga ggccaatatg tctggcgata aaatcgacca tggcggctgt
     9181 gtgaatacgc taaccactct gcgcccttca ccactggcga agggaaaccc gcagcacact
     9241 aatctggtcg agatcgaaaa aatataaccc acgacaacca taaattctgg catgacattt
     9301 tgtttgaaaa gcaataagtg agtaatgatg aaaatccata ccacagaggc gctcatgaag
     9361 gctgaaatca gccgtagaag tctgatgaaa acctccgcac ttggcagtct tgcgctggca
     9421 agcagtgctt tcactctgcc attttcccaa atggtccggg cggcagaggc tccggtagaa
     9481 gagaaagcgg tctggagttc ctgcaccgtt aactgcggga gccgctgtct gttacgtttg
     9541 catgtgaaag atgacaccgt gtactgggtg gagtctgata cgacaggtga cgacgtctac
     9601 ggtaaccatc aggttcgagc gtgtttacgc gggcgctcta tccgccgacg gatgaatcat
     9661 cctgataggt tgaaatatcc catgaagcgc gtcggcaagc gcggtgaagg taaatttgaa
     9721 cggataagtt gggacgaagc cctggatacc atcagtgata atcttcggcg gatcctgaaa
     9781 gattacggca atgaggctgt acatgtcctg tacggaacag gcgtagatgg cggaaacatc
     9841 accaactcaa acgtcccgta ccgtctgatg aactcttgcg gtggttttct cagtcgctat
     9901 ggcagctaca gtaccgcaca gatcagtgcc gcaatgagtt atatgttcgg tgccaatgat
     9961 ggcaacagcc cggatgatat cgccaatacg aaactggtcg ttatgttcgg aaataacccg
    10021 gcagaaacgc ggatgagcgg cggtggtgtc acttactacg tcgagcaagc ccgcgaacgt
    10081 tcaaacgcac gcatgatcgt catcgatcca cgttataacg acactgctgc cgggcgtgaa
    10141 gatgaatggc tgcccattcg ccctggcacc gatggcgcac tggcctgtgc gattgcctgg
    10201 gtactgatta ctgaaaacat ggtcgatcag ccatttctcg acaaatattg tgttggttac
    10261 gatgaaaaaa cgctgcccgc caacgcacca cgtaacgcgc attataaagc ctatattctg
    10321 ggcgaagggc ctgacggcat agctaaaacg ccggaatggg cagcaaaaat caccagcatc
    10381 ccggcagaaa aaattatcca gttggcacga gagatcggtt cagcaaaacc tgcttatatt
    10441 tgtcagggtt gggggccaca acgacattcc aacggcgagc aaacgtcccg cgctattgcc
    10501 atgctttccg ttctcaccgg taacgtcggc ataaacggcg gcaactcagg tgtacgcgaa
    10561 ggtagctggg atctgggggt agaatggttc ccgatgcttg agaatcctgt taaaacgcag
    10621 atttccgtct ttacatggac agatgccatc gaccatggta cggaaatgac cgcgacccgt
    10681 gatggtgttc gtggaaaaga aaaactggat gtccccatca agtttttatg gtgctacgcc
    10741 agtaacacat tgatcaatca acatggcgac atcaatcaca cccatgaggt gcttcaggat
    10801 gacagcaagt gcgagatgat tgttggcatt gaccacttca tgacggcctc ggctaagtat
    10861 tgcgatatcc tgttgcccga cctgatgcca acagagcaag aagaccttat ctctcatgaa
    10921 tctgcaggga atatgggcta tgtgatcctc gcccaacccg caacctcagc aaaatttgaa
    10981 cgcaaaccca tctactggat gctgagtgaa gtcgccaaac gcttaggacc agacgtttat
    11041 caaaccttta ctgaaggtcg cagtcagcat gaatgggtca aatatctcca tgcgaaaacg
    11101 aaggaacgta accctgagat gcccgactac gaggagatga aaacgaccgg gatctttaag
    11161 aaaaaatgcc cggaagaaca ctacgtcgct ttccgcgcat tccgtgaaga tccacaggca
    11221 aacccgttga aaacaccttc ggggaaaatc gaaatttatt ctgaacgact ggcgaagatt
    11281 gcagatacct gggaattgaa aaaagatgaa attattcatc cccttcctgc gtatacccca
    11341 ggttttgatg gctgggacga tcccctgcgg aaaacctatc cactgcagtt aacgggcttc
    11401 cattacaaag cgcgtaccca ctccagctac ggcaatattg atgtgttaca gcaggcctgc
    11461 ccacaagagg tgtggatcaa ccccattgat gctcaggcac gcggtatccg tcatggcgat
    11521 accgtgcggg tatttaacaa taatggagaa atgctgattg ccgcaaaagt gactccgcgt
    11581 attctgcctg gcgttaccgc catcgggcaa ggtgcgtggc ttaaagcgga tatgtttggt
    11641 gaccgggtcg atcacggcgg cagtatcaat attctgacct ctcaccgccc ttcaccgctg
    11701 gcaaagggaa acccgtcgca cagcaatctt gtccagatcg aaaaggttta aggagtagcc
    11761 catgaccaca caatatggat tttttatcga ttccagccgc tgtactggct gtaaaacctg
    11821 cgaactggcg tgcaaagatt ttaaagatct tggcccggaa gtcagtttcc gccgtattta
    11881 tgaatacgct ggcggcgact ggcaggaaga taatggcgtc tggcaccaga acgtgttcgc
    11941 ttattatctc tccatctcct gtaaccattg cgatgacccc gcatgtacaa aagtctgccc
    12001 cagcggcgca atgcataagc gtgaagatgg ctttgtggtg gttgatgaag atgtttgtat
    12061 cggctgccgc tactgccaca tggcttgtcc gtacggcgca ccacagtaca atgctgaaaa
    12121 agggcacatg acgaagtgcg atggttgtta ttcgcgcgtc gccgagggga aacaacccat
    12181 atgcgtcgaa tcctgcccgc tgcgtgcact tgagttcggt ccaattgaag agctgcgcca
    12241 gaagcacggc actctggctg ccgtcgcgcc actgccgcgc gcgcatttca caaaacccaa
    12301 tatcgttatc aaacctaacg ccaacagccg cccgaccggc gataccactg gttatctggc
    12361 taatccggag gaagtgtaaa atgggaaatg gatggcatga atggccactg gttatcttta
    12421 ccgtattggg tcaatgcgta gtaggcgcgc tgattgttag cggaattggc tggttcgccg
    12481 ccaaaaatga cgcagaccgt cagcgtattg tccgtggcat gtttttcctc tggctgttga
    12541 tgggcatcgg gtttattgcc tctgtcatgc acctgggatc gccactgcgg gcgtttaact
    12601 cgctgaatcg aatcggagct tctggtctga gtaacgagat tgctgccggt tccatatttt
    12661 ttgccgttgg cggactatgg tggctggtgg cggtcatcgg taaaatgccg caagcgttgg
    12721 ggaaactctg gttacttgtc agcatggcgc tcggcgtcat tttcgtctgg atgatgacct
    12781 gcgtgtatca aatcgacact gtgccaacct ggcataacgg ttacactacg ctggctttct
    12841 tcctgacggt attgttgagc ggtccaatac ttgctgccgc aattctgcgg gcagcccgcg
    12901 ttacatttaa taccacacca tttgccatca ttagcgttct ggcgctgatc gcttgtgccg
    12961 gggtgattgt gctgcaaggt ttgtctctgg cgtctattca ctcatccgtg caacaagcca
    13021 gcgcactggt accagattac gcctccttac aggtctggcg tgtggtattg ttatgcgcag
    13081 gtcttggatg ctggctttgc ccgctcattc gtcgccgtga acctcacgtt gccggactta
    13141 ttctggggct aatcctcata cttggcggtg agatgatcgg gcgtgtgctc ttttatggcc
    13201 tgcacatgac agtaggaatg gctattgcag gttaacaaat tggtgcgggg caacccgcac
    13261 atttcaggat gttaggaatg acccattttt cacagcaaga taatttttct gtcgcggcgc
    13321 gcgtgttggg cgcgctgttt tattacgctc cagagagtgc ggaagccgca cctctggttg
    13381 cggtactcac cagtgacggc tgggaaactc agtggcctct accagaagcg tcattagcgc
    13441 cgctggtgac ggcttttcag acacagtgtg aagagactca cgcccaggcc tggcagcgtt
    13501 tgtttgtcgg cccgtgggca ctgccgtctc cgccgtgggg gtcggtctgg ctggatcgcg
    13561 aatctgtgct gtttggcgat tcaacattgg cacttcgtca gtggatgcgc gagaaaggca
    13621 ttcagtttga aatgaagcaa aacgaaccgg aagatcattt tggttcactg ctgttgatgg
    13681 ctgcgtggtt ggcggagaat ggtcgccaga cagaatgcga agaattgctg gcatggcacc
    13741 tttttccgtg gtcaacacgt tttcttgatg tttttatcga aaaagcagaa caccctttct
    13801 accgtgcact gggtgaactg gctcgcttaa cgctggcgca atggcaatca caactgttaa
    13861 ttcctgtcgc ggttaaaccg ctgtttcgat aggatcaccg taatattgcc ggatggtgac
    13921 gattacgcgc aatccggcaa taataggtta cagtgtcacg tttttttatc tcttaaagca
    13981 cgcactgctt ttgcggctgg cctcttttgc cgcaaaatag tcgcccgtgt ttcattgccc
    14041 atttctgctc atgcatcatc tacacatcta tccggatctg cgcactatgt tccgccgtct
    14101 gcttatcgca acagtcgtcg gtattctcgc ggcctttgcc gttgccgggt ttcgtcatgc
    14161 gatgctgcta ctggagtggt tgttcctcaa taatgactcc ggcagtctgg tcaatgcagc
    14221 gacaaacctt tccccctggc gacggttgct aactccggcg ctcggcggac tggcggcggg
    14281 tttgttgctg atgggctggc agaaatttac ccaacaacgc cctcatgcgc cgaccgatta
    14341 catggaagcg ttgcaaaccg atggacagtt cgattacgca gcaagcctgg ttaaatcgct
    14401 tgcctctctg ctggtagtaa ccagcggcag tgcaattggt cgcgaaggtg cgatgattct
    14461 tttagctgcc cttgccgcct cctgttttgc ccaacgtttt acgccacgcc aggagtggaa
    14521 attatggatc gcctgtgggg ccgcggcggg aatggctgcg gcctatcgtg ccccgcttgc
    14581 tggcagttta tttatagccg aagtgctgtt tggcactatg atgttggcct ctctcggccc
    14641 ggtgattatt tccgccgtcg tggcattgct ggttagcaat ctgattaatc atagcgacgc
    14701 gttactctac aacgtacaac tctcagtgac ggttcaggct cgtgactatg cgctgattat
    14761 cagtacaggt gtgctggcag gtctgtgcgg accactgttg ttaacgttaa tgaacgcctg
    14821 tcatcgtgga tttgtgagtc tcaaacttgc gccgccctgg caactggcac taggcgggtt
    14881 gatcgtgggt ctgctttccc tgttcacacc tgcagtgtgg ggcaacggct atagcaccgt
    14941 acaatccttt ttaaccgccc caccactgtt aatgatcatt gccgggatct tcctttgtaa
    15001 actgtgtgcc gtgctggcga gtagcggttc cggcgcaccc ggtggtgtct ttacaccgac
    15061 gctatttatc ggtcttgcca ttggcatgtt gtatggtcgt agcctgggat tatggttccc
    15121 tgatggcgaa gaaattacgc ttttactcgg attgaccggg atggcgacac tgttggcggc
    15181 aaccacgcac gcgccgatta tgtcgacgtt gatgatatgt gaaatgaccg gggagtatca
    15241 gctactcccc ggtttattga ttgcctgcgt cattgcgtcg gtaatttcgc ggacgttaca
    15301 ccgtgactct atctaccgcc agcacactgc gcagcatagc taagcggatg tattgcccca
    15361 gttctcgctg ttcggcgcgc ggcagataag gcagttcacc aatgagcggt gccggaagtt
    15421 ttttacccag cacatcaatg atttccgcat aatgcgccag tcctgggttg attcggttag
    15481 ccacccagcc aatgagcggc agcccgtcgt tggcaatcgc ctgagctgtt agcagtgcat
    15541 ggttaatgca accttcctga ataccgacaa ccatcaacac cggcaactgt tcctgcacca
    15601 cccattcaga gagtggacgc aaatcattca tcagactgcg ccagccgcca gtcccttcta
    15661 ccacgacatg atcgacttta tcggtcaggt ttgccaggcc gtttgaaatg agggtgtaat
    15721 tgattgggca actgtgcgcc acgctacttt cttcttcgct taacgcgata ggattaactg
    15781 cttcataagg cagttcgatg gttgaaacac tctgcaacac cagggcatct ttattacgca
    15841 gcccttcggg tgtctctttg ctccccttcg ctacgggttt atatcccgca accgtttttc
    15901 cctgggaggc taacgcttgt agcaatgcgc gggaaaccac cgttttccct acagaagtgt
    15961 ctgtaccggt aataaagaaa cgcttcagca tcactaactc caccgttatg cttcacaaat
    16021 ataaaccagg aaaataatta accttgaaag tctaagttat gctttcctgg cccaaattga
    16081 gatagcgcaa attttggtag aacagttaaa aaatgttaac cctgcaacag acgaatcaac
    16141 aaagaaccgt tatacatcgc gtcttttacc agtgcagcgc ctgccatcgt gccctggtta
    16201 gaaaactgag tactctcaac gctgatgtgc tgactatacg caggaagggc ctgctgacgg
    16261 atgctgtctg agatgaccgg gaagaggata tctgccgctt tacttaacgg tgagccaatc
    16321 agtatttttt gtgggttaaa taaattcacc atgatggcaa gaatgcgccc tacatgcgcg
    16381 cccaccccgg taatgatgtc ttttgccagc agatcgccgc gcaatgccgc ctgacacaat
    16441 gagtccacgg ttaacggttg tccatgtaac atcgagctca tggattgatt aagacgcagc
    16501 tgtgccagct caagaatact gtccacactg gcgatggttt cgaggcagcc gtgattcccg
    16561 caataacagc gtttcccata cgggtcgacc tgtgtgtggc ctatttccac gagactactg
    16621 ctgcctgcgt gtagcagatg accatcggta atgacgcccg cccccacgtt gtgatcgata
    16681 accacctgaa tcacatcgcg cgccccgcgt gaggcaccaa acaaggcctc tgccatcgtc
    16741 catgcgctga tatcatgctg aatataaacc ggtacgccgg tatgctgctc cagcgcctcg
    16801 ccgagcggca tctcttttac atcctcgtag aacggcatgc gatgtacaat accattttcc
    16861 gtatcaataa ttcccggcaa ggttatggca atcgaagtta gacgctcaag ttttttctgg
    16921 tggcgaataa aaaactgatc gatatgggaa atgatacgat ccagcaatgg cgagtcatct
    16981 tttaacgcca gttcctgcga ctcttccacc accagtttgc tgctcagatc gcgcagagca
    17041 aggaaaatct ccccgcgact aatgcgcaga gaaagatagt gccaggcttc agtttcaacc
    17101 accagcccca ccgccggacg gccacggttc cccgcttctt tgatttccag ctcttgcacc
    17161 aggtgtgctt cgagcatctc acggacaatt ttagtgatac tggcaggagc cagctgcgcc
    17221 agacgggaaa gatcgatacg cgagactgga ccaagctgat caatcaggcg ataaaccgcg
    17281 cccgcgttgg tctgctttat ttgatcaatg tgcccaggct ggttttcagc aaccaccgca
    17341 tactccctat attttcgcgc tccgaaataa tctgtaggct atggtgaagc acttcaatac
    17401 gtgtcgtcaa atttttactt aggcatgtga ttaacagcac atttttcggg ctttttcgct
    17461 gaaatttccc tcacctgaga gcattcagca gatgaatacg aaagttacgc gcagccggac
    17521 tttgttcatg atgtttcggc cagaccaacc acatttcaga aaccgcatcc tcttcagcaa
    17581 tcggcaccca gcgcatttcg ttgagctgaa cacgtttaaa tgaagcaggc aaaattgaaa
    17641 cacccagacc ggcggaaacc agaccgatga tggtcattgc ctcgcccacc tcctgagtga
    17701 tgacgggcgt caaatggtaa cgtcgcatca gcccgagaat atcgtcatac agccctgtcc
    17761 cgacgtgcgg atcaaaaaag acgaagggtt ctttcgccag ttcagccagc gttacattcg
    17821 ggttatttgc caggggatga tcgtgcggga tcatcgccat aagcggttca tggacgatga
    17881 ctgcgtgttc aagcgtctcc ggtaacgctg tgtaacgcag caatcccata tccagcgttc
    17941 cttcaatgag cggagcgatt tgctcacgag tgttcatttc gcgggtttgt aaatggacat
    18001 cgggataatc acggcgaaac agcgataacg tatcggacac cgcccgaata aaaggagcgg
    18061 acgaagtaaa accaatgcgc aactcccccg cttccccctg atgcagtctt tcagcgcgag
    18121 cggcagcgtc atctaccata gacaggattt gccgactatc tgcaagaaac tgttttcctg
    18181 ctgccgtcag caatacactg cgattagttc gtgccagcag tcgggcacca atttgttgct
    18241 ccagtgcctg aatctgctga cttagcggtg gttgcgaaat attcaggcgg gcagcggcgc
    18301 gcccgaaatg cagctcttcc gcaacagcaa caaagtaacg cagatgacga agttcaatat
    18361 tcatatttaa aacatcttat ttgagattat taatatatta gacagaacaa ttcgattttc
    18421 ctaacctatg tataagcctg atccacaggc atatttagca aggatttcaa gtgagccgta
    18481 ctacaactgt tgatggcgct ccggcaagcg acactgacaa gcaaagcatt tctcagccaa
    18541 atcaatttat taaacgcggt acgccgcaat ttatgcgcgt caccctggcg ctgttctctg
    18601 ccggactggc aacatttgca cttctctatt gtgtgcagcc tatccttccg gtgctttcgc
    18661 aggagtttgg cttaacgccc gcgaacagta gtatttcact gtccatttcc acggcgatgt
    18721 tggctattgg tttgctgttt actggccccc tatccgatgc cattggtcgc aaaccagtga
    18781 tggtcacggc gctactgttg gcctccattt gtacgttact ttcgacaatg atgaccagct
    18841 ggcacggcat tttgattatg cgcgccttga ttgggctttc gttaagtggc gtggcagctg
    18901 ttggcatgac ttatcttagc gaggaaatcc accccagttt cgtggccttt tcgatggggt
    18961 tgtatatcag cggcaactca attggcggca tgagcggacg cttaattagt ggtgtcttca
    19021 cggacttttt caactggcga attgctctgg cggcaatcgg ttgtttcgcg ctagcctcgg
    19081 cgttgatgtt ctggaaaatc ctccctgaat cacgccattt tcgcccgact tcgctgcgac
    19141 ctaaaacgtt gtttatcaac tttcgtctgc actggcgtga ccggggatta ccgttattgt
    19201 tcgcagaagg ctttttgctg atggggtcgt tcgtcacgct gtttaattac atcggatatc
    19261 ggttgatgct ctccccctgg catgtcagtc aggccgtggt tggcttatta tcgctggctt
    19321 atttgaccgg tacatggagc tcacccaaag ccggaaccat gaccacccgc tacgggcgcg
    19381 gtccggtgat gttgttttcg acgggggtta tgctgtttgg tttactgatg accttattca
    19441 gctcgctgtg gctgatcttt gccggaatgt tactcttctc agcaggattc ttcgcagccc
    19501 actcagttgc cagcagctgg attggccccc gcgcaaaacg cgctaaaggc caggcctcct
    19561 cgctgtatct gttcagttac tatctggggt cgagtattgc cgggacgctg ggtggtgttt
    19621 tctggcataa ctatggctgg aacggcgtcg gcgcatttat tgctctgatg ctggtcattg
    19681 ctctgctggt cgggacgcgt ttgcatcgtc gtctgcacgc ctgaaaaata agcccggact
    19741 gcggtaacta cacgtccgga cttattgctt gctcaaacca acgttaatag ccatcctaaa
    19801 atagacgaag cgccagccaa ttcccgcagc gcgtctagcg tcatcaggat tataagtacc
    19861 caaataaacg gattcatttt gctgtgtgtc atttattact gatgcgcagt tattctaccg
    19921 ctttgtaagt agtaaaatag ttaacccgat caagactact attattggta gctaaatttc
    19981 ccttaagtca caatacgtta ttatcaacgc tgtaatttat tcagcgtttg tacatatcgt
    20041 tacacgctga aaccaaccac tcacggaagt ctgccattcc cagggatata gttatttcaa
    20101 cggccccgca gtggggttaa atgaaaaaac aaattgaggg tatgacaatg aaaaaagtat
    20161 tagctctggt tgttgccgct gctatgggtc tgtcttctgc cgcctttgct gcagagactg
    20221 cgaccacacc tgctccgact gcgacgacca ccaaagcagc gccggcgaaa acgacacatc
    20281 ataaaaaaca gcataaagca gcacctgccc agaaagcgca ggcggctaaa aagcatcata
    20341 aaaatacgaa agctgaacag aaagcccctg aacaaaaagc gcaggcagcg aagaaacacg
    20401 ccgggaaaca cagccatcag caaccggcaa aacctgctgc acaacccgca gcgtaagttt
    20461 tcaacagtaa tgctggcgcg ccccctcgcg cctgaaaatt acggtgctaa gcaggtaacg
    20521 tttagcaccg cctttagcgg agggtagtat ggtgggccgt tatcgctttg agttcattct
    20581 tatcatcctt attttatgcg cactgattgc cgcccgtttt tatctttcct gactgtagtt
    20641 atctgatttt actcccactt tcatcccgtc ccgtctatag tatttatgag ggtttgcttt
    20701 taataatcat aattacccac cagagtgtga tatgcgtaca accattgctg tagtgttggg
    20761 tgcaattagt ttgacgtctg cttttgtgtt tgcagataaa ccagacgttg ccaaatcggc
    20821 aaacgatgag gtcagcaccc tgttttttgg tcatgatgat cgtgtgccag tgaatgacac
    20881 gacccaatca ccgtgggatg cggttgggca actggaaacg gccagcggca atttatgtac
    20941 ggcgacgctg attgcaccca atctggcatt aacggcagga cactgtttat tgacgccgcc
    21001 taagggtaaa gcggataagg cggtagcgct gcgttttgtg tcaaataaag gtctttggcg
    21061 ctatgacatc cacgacatag aaggccgcgt tgatccgaca ctgggaaagc ggttaaaagc
    21121 agatggggat ggttggattg ttcctcccgc agctgcacca tgggacttcg gcttgattgt
    21181 gctgcgtaat cccccttctg gcattacacc gttgccgtta tttgagggag ataaagccgc
    21241 gcttactgcc gcattaaaat cggctggtcg taaagtgact caggcgggat atcctgaaga
    21301 tcatctcgat acgttataca gtcatcaaaa ctgtgaagtg actggctggg cgcaaacgtc
    21361 ggtgatgtca catcagtgcg ataccttgcc gggtgacagc ggttcgcctt tgatgttgca
    21421 taccgatgac ggctggcaat taattggggt gcaaagttcg gctcctgccg cgaaagatcg
    21481 ctggcgcgcc gataaccggg ccatttctgt taccggtttt cgcgacaagc tggatcaact
    21541 gtcgcaaaaa taatgttcaa acgctgcccg acagtgcggg cagcgtgttc atcaggcaag
    21601 tttcaccatg atcattccag ccaacagcaa gaccaggcca atccagcctt tacgatttaa
    21661 ccgttgacca aacaagatcc aacctgcggc taacgtggcg gcaataccaa acccgcccca
    21721 caatgcataa gcgacagaca agtcgatccc ttttacggcc tgagaaagcg cactaaaggc
    21781 agccagcacc gccgccaggg agagcaagcc aaatattttg cgacgaaagc cgtcagaaaa
    21841 tttcaaaaag acgttagcaa cgatttccag cacgattgcc aatgccagcc aggcggcgtg
    21901 aacccattca aactgcgcca tggttcactt ccagttcagg tttacgcgct ttacgggtac
    21961 ctgatttgat caacacaatc ccggcgacca gggtggttaa cccggcaatt ttcatcagcg
    22021 ataaactttc gtcgaataac aaaacgctaa acaaggtaat aaataaaata ccgatacctt
    22081 cccacagcgc ataagctacg cctaaggcga tctttttaac ggcgaaagag agaaatatat
    22141 acgacagaga aatcatcacc agcattaaaa taaagccgcc atttccctca ctgacgctcg
    22201 cccatttcat tgacagcgta ccggtaattt ctgtagcaat agccaggcct aataaaatcc
    22261 aataaatata cattgtcctt ctcctgcaag agaattattt taattttcgc ttaattcagc
    22321 gaaaccaaag taaagtggtc aagccccgcg catagggcaa gcttaggcag aagaaaggac
    22381 taaagcgcgt tgcgccagtg ctgctcacct acgagcaaga tagtggatga ggtacgaaga
    22441 tgggtaaatg ttgaaaataa cgtcctgaac aaattgtcca taatattaca attatccgca
    22501 gtgttgcttc tcgtcatcgc ggatgataat tgtcctcggt agttgaacac gcctgatttg
    22561 tatcatagct taagaattaa ctcaaaatat tttcacttct ttacctgagc ggtttgattt
    22621 tcgttatgat gacggagcga aaaagacatt attattagca aaggaagaaa aaacggggac
    22681 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
    22741 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
    22801 cgcattattt attgctgtta tatataactt atttcacata acacgttgat ttaattaaat
    22861 aattttcacc gcaaaaagtg ccaaaaatcg caaaatgact acaccagtga ctacaccgtt
    22921 cggtgcactg tatgaaacaa cgtggaacaa atagacacaa gaaatataca ggcggatcat
    22981 ctttccaggg ggaagcgcac caattcatga ggggcgttaa tgtcgatatg gggatcccca
    23041 cctaccggaa tttttttccg gttaaacgtt aatcaggctg gtgggcttta cctgttcggt
    23101 aggtgttatg attatcgtaa tacctttccc ccaatggggt aaaagcatca accgtaacga
    23161 aagtcgttat ggttgccact gataccccaa atttgggtat cacgagtaag acccaaaatc
    23221 tgggtgttac tcttactacc caaactacgg gtagtttccg tagtttcatg gtcgagttgc
    23281 agatctgcaa ctcccccatt aaccacaggc gaatttccgc cttcggtcat tccttaatca
    23341 tctgcgtaat cataatgatt attcagcaag cgcaattttg cactttgctt aaagttcagc
    23401 gagttaccgc gccgacttcc tcaaattgag gtttccgaaa atatcagcgg gttagcgatt
    23461 aagctgatca ttaattaaac tgcgaaaatt tcgtagtttg tgactgtctt tcaggcagct
    23521 tgttaacagt cctcagaagc gagggatgta gccacccatt tttggtccgt cgggaatatc
    23581 aaccagttac cgccgcaacc gctccggctt cttccagtgg tacgttattt tctccttctc
    23641 ccgatacatc tccacgcggc gacggtaggc cagcaactca agagctctgg ttcgtatgtt
    23701 gcgcatatcc acgccattaa gttctatacc atcacggcgc atcacctcag caacaacacg
    23761 cgcatagttt tcggctgtca cgctgtccgg ctgcgtggcc tgttcgtcag cctgctggct
    23821 gattccggca acgcggcgga ttaatcccgt tatttcggct tctgtcatgc tgctgacctc
    23881 attacacccc gctaaattct tccagtttct ggcggtggct gtcgcttata tcaaaagcaa
    23941 aatcctcatg ctctgcctgg aatgtaccaa acgccatcag cgccgccacg ctcgggtcta
    24001 tcttgttcgg tgattttttc ttgttcggct tgatattggc gttcgcgtca ctctgcatca
    24061 cgacgttact catcgaccag gacaacaccg gatcgccacg atgcacaatc accctgcggt
    24121 taacaaaaac ttcgaacgat ttcgccgccg gactgaatct gaggtaggtt tgcgggaacg
    24181 gctccacctc aaaacctgcc ccctgtaatt gcgttctgag atgcgtggcg ttccacgtat
    24241 caaagcccac cagcctgata ttaaatttct ccgcgtcctg catgatgtca tctctgatcc
    24301 ggtcgtaatc aatgcagtcg ccaggcgttg tgcgtatcca gcctgcttta gcccactgac
    24361 gatagatggc gcggttttta ttggcggggt tctgtagctg aaattccggc agatagtgac
    24421 gggaaaccag cataatggtt ttaccgaccg gaaaggcata gcacacgctg gaaatatcgc
    24481 tggttgatga taagtccagc cccgcgtaac actcctggcc gtgtaaatct tcctccgtga
    24541 acgttccggc acactcagcc catgcgccgt tacccatcca cggggtagcc ccctggcacc
    24601 agatattaaa tcgctttgtc atcatctcca cccactgcga cggaataccc cgcgctttct
    24661 ggatggttga ggccagtttt tcacgatcca cggaaacatc aatattaggg ttcgccttta
    24721 tccacatcgc cggatcatca acctcgtttt cgtcgtccag ttcgtaaatc agtacaaaaa
    24781 ttgaatcgtt gacctcttcg ccgtccagga tctggcagca ataatcatag tgctgcttac
    24841 aggctgaaac gacgttactg cctgatgtgg taatagcaaa taacagccct tcggggcgtg
    24901 cgcccatccc cagctcaagc gcggaataaa cgccgttatc ggggtgcaag tggtattcgt
    24961 ccacgatgga aaggctgggg tttgttccct cgatggttgc cgcttttgct gccagcggct
    25021 ttaacaggct gttgcttttc gggtgcatca ccttatgcgc ctgaatattc acccgcctgc
    25081 gtaacggtcg ggataaaagg cacatctgac gcgcatcatc aaagacgatc cgcgcctggt
    25141 cacggctcac cgctgcggtg taaatatcct gctgaccgtt ctccataatc agaaaccagt
    25201 tagccagaat cgcggcggtc gtggatttcg catttttgcg cggcacttcg ataaaggcgc
    25261 tcgtgtattt gcgccgtccg gtggccttaa ccttaaagcc gaggatgcac gcaaaggcga
    25321 actgctgcca cggctccagc tcaatgggtc tgccacgcat cggccctttt acgtgcgggc
    25381 acaccctgga aaaggcaata aaccgctcca caacctcacg atcgaacgtg taaagggggc
    25441 tttcaaggtc cgaaaagtac cgtttaacgg cctgtttcag ccgtttacag gccggaattt
    25501 tgcccgtttt tacgtcttct gcgtacttat tccaggcggt caagctcgtc ctcttcttct
    25561 gtttccggtg gatttttacg gcggcttatc gggtcaaaac cgagcaagga ggccattttt
    25621 atcatcaccc tttcagcgtc ggattttgcg cttaatgcgg ggtttctgct ctcgccgccc
    25681 tgactgttaa caatgctgaa cccgcgcgcc gcaaggtctg caacggcttt ccggtaaatg
    25741 gagtagttga cacaatacag ttccagattg ctccagtcgg cgggggtcag gtctccccgt
    25801 tccgcaagct gccgcgattt ttcccgccac tgcttcacgg cgatgtcatc caggtaggcg
    25861 ggggctttcg gtggtcttgc catacttatt ttttcgccag attatttttc aaaaaattcc
    25921 cgtgcataaa aatttgagga ggcggtcggt gcccggcggg gtctggtttg tcctgaaaac
    25981 gcccccaccc cgtcatacag cctcatcagc gattgcgaaa acattccata acctcgcggt
    26041 cacggtcggt taatcgcttc gctgtgatgc gttctgcgcg tcctgacgct ttatctttat
    26101 gccctgtttc ctgtgtcttc catgcgtcac gctgccttat aagtccacgg ataaggcggt
    26161 tttgttcccg ctcattcatc agcgtcatac atccagttat tgcggttagc ggcccgttct
    26221 tcctcttcac gaaatccacc tgcggcacgc ttgctttttg ttgccggatc aagccatttc
    26281 gttttctggt tatggcacgc ctgacacaat ggttgatggt tccattcggg ccagaagaga
    26341 acatcatcac cgccatcgat ggggataatg tgatccacca ccacggcggg cgtatatatc
    26401 cccttctcaa ggcatcgcac gcataacggg tttttactca gatacatggc gcggtatttg
    26461 tcccactgtc tggagtaccc acgcgcgcgg cggtgtcctc gtctggcatc ctctgcacgc
    26521 catgccgccc gcctgtgctc ctcacacttg ccggacttca cgcgcctgtt acagcctggt
    26581 tctgtgcatc gtcttaatgg ttgccacggc atcagtacac ccccacatca cggtaagccg
    26641 tccagagtgc accaatcgtc atgggtacgc gcgtttttgc gttatccgct accagctggc
    26701 gattctcgta caggtgagcg ataaacatca tgcagccaat ctttatggct ggcgtgaact
    26761 ccagcccgtc atcaaagcgc ctgcctatat gtatctggca cgcctcaagc gatgcggcaa
    26821 tgtatccgct gattaactgg tcttcctcgt cgccatcgat gcggcaatgg agtttcactt
    26881 cttccagggt gataagttct tctgtcattt ttccgcgccc tcacgacaaa gaatttcaag
    26941 gcgtgtcctg gcggcatccg gcagcggctg cccgatgata ttcagtacac gccccgccag
    27001 cggccccgta ttgaccttta tccggctggt ggcgttgatg tccttcctgt agcgtatcca
    27061 tatccttacc gttccagtcg ccagttctgc acccgatgaa atggcctcct tgctgctgat
    27121 catgttcacg cttgcccaga gtgtgtgacc gtccacccac gtttcgagta tttcgccggt
    27181 tatatccctg gtctgtttca gggtctgaat cgttaccctg tcacgcagtc gccctatgtt
    27241 cattccggtt tttctccctc gctgattttt acttcctgtt tccatgcctg gctgaactca
    27301 tcaccaccat cacgcgggga cagcccttca cgttcgcggg cttcgttcgg gcacataacg
    27361 ccggatttga tgccgcgttc ataggtggca aagcgttcgc caggtgtggc ccgtaacagg
    27421 tccgcgctgt caaactccac ctgataccgg ataccaggta caggtgaggc caccagcagg
    27481 gcgtttttta tctgctgctc aaagttcgcc agccacgggc gcatcgtcat ggtgagaaat
    27541 gcgcggcttg cctcgctgaa attgctgtag gtgctgttgc tgtattcctg cagaaaaatc
    27601 ggcgagacgt tgaacattcg ggcgatgtct tcaatggtga agcgacggga ggccagccat
    27661 tcagcatcct gattactcat gcccagctgc tgatagctca tacccccttc aaggatgggc
    27721 gtttttccgg cgtttctggc ccctttgtag cgttccagtg cggctaatgc ctgtttgccc
    27781 ttcacgccgt ccagccattc gcctgacgtg ataacccctg ccgccatcat gccatcacgc
    27841 atcacgctcg cgccgtggcg ctgttgggcc agcccaagcc ccagcgattc tcggcagatg
    27901 gttacaggtg agcgccccat aaaaccgtca tccgtggagt aacgaaggtg gagaacctcc
    27961 cacggtaaat agttgcgggt gtttccggtg taggcgtcag tgatgcagta gcgccagtta
    28021 tgctcacctg tctgctcaac gttcaccgac tgcggcgggt aaggatgcaa agcgaccgga
    28081 aaaccatcac gcccccactg aatcaccgca taagcattac cgtttaacag gcagtggcgg
    28141 atcatcattc gcttaaactg gtagggggtc tgccatgcgt tcgggcgctc gttgaggatg
    28201 tgatcgaccg gatgagaatc aagccactcg cgggcctcct tccccttctc attgcgtacc
    28261 aggtacaggt aacacggcat ggtagccacc gcctcagaga tgaccgtcac ggcgttcatg
    28321 acggcgggca gtgattccgc tgtccccgat gatacgtact cacccgcccc cgtgttcgat
    28381 gttccagcca gcgccataaa cccatcaagc gtcatgctgc gctgctcttt ttttcttctg
    28441 aaaggccaca tatcacaccc ccgctaaatc cgcccaccag cggcgattat ccgcacgcgg
    28501 cattttttcg gggtgctgct cgtacaggga gcggcgggcc agctccacgc cagaatcagg
    28561 gtaagccggt acggatgtaa cggtaatttc gtacagttcc gccaccagca cggtgcgcac
    28621 gcatggatct gttgtggtat cccatacatc cttacgggag cgaaagccaa agctcatgcc
    28681 ggatatatca cctcgtttaa ccagttcgat aacgtcccgc cctgtactgg tatccggtgg
    28741 ggtcagttca aagcgtaacc ctgtgtcgtc ctcttccagt ttcagcgtgc cggaacgggt
    28801 gcgccccagt aacatgctgt ggtcatgctc atacaggccg cgaacgtcat tacccgctgc
    28861 aagccattca gtaaacgctc cccgctggaa tttttcgtaa aactcccccc atagcagctc
    28921 tgaaaggtta tcccagcgaa caacgtagcc tgtaagcgtg ttgctggcgc tggtggtgat
    28981 ttccgatgac cggatttcca tactcttcat aattttttca cccataaaac actaaagggg
    29041 cttttaagcc ccttctgtat gctgttaatc gtcgtcctgt ggcagttcca gaatcttgat
    29101 cgcgttcgaa tccaccacgc caccgcctaa atatttctgc gcgaaaattt tgatgaagcc
    29161 cggctctgtg aggttgtccg gtctggtgcg aacacctgtt tcgtgatcaa caatgtagta
    29221 accgcgtttg aagtcaccca gggcaataac gttatccggc ataaactcca gatattcgac
    29281 cggaaggccc agcaacgtat caggatcacc cgcctgtaaa cggtcacgcc agatgtaatc
    29341 accgttcgcg ttcttcacct tctggagttt tgccgccgtc gtggagttaa ccacccagac
    29401 cgcgtttttg cggtattttt tacgtaatgc aaatttcagg tcgatcagcg ggtccgcaga
    29461 tgtccacgcc agagattcgg aaggtttaat tacctgtaac gtaccaaaat cacgctcttt
    29521 gtcgttcttc tctgcacggg gtacggataa aaagcctttt gcttttttgt caccatcgcc
    29581 cacaaccaga tcgctttctt cggtttccgt gaaggtgtcg ccaatctcac ccgtcagcca
    29641 tgaaaggatg tccacatcgg aaaaatccac gatttcctgt gtggtgcgcg ggtacgcata
    29701 gaccggatac agcttaatgc tcacctcgtt aatctgcggg gtgctggtct gttcgcgtgc
    29761 cttaccctct tcaccgtggt taacggtcgc accgccagcg gaaacaagct gcttaaactc
    29821 gttgctgctg attttcttca cggtacagat gcggcgcatg gtggattcat ccgccagcat
    29881 tcgcatgatt tcggtgttca gttcagggat aacggtataa ccaccatcag cgggaacgcc
    29941 tgtacttaat gcgcgggttt cacctgtcag aatgtagttg cggagttctg cggggtcagt
    30001 ggtctgactg cttttacctg gcttgctgcg ctcttcgtct gcaatggctt caaggcggga
    30061 aatgtcttta tcgagggatt cagctttagc gcgtaattcg tcaaatgttg cgccctcagc
    30121 atcgttaaga ctgcggtttt ctttttccgc gttctccagc atgtcgcgca tctgattttt
    30181 aatggcggtt ttctgctggc gtaattcgat tattctcggc ataaaaaagt cctggggtta
    30241 agtaaggaac tccaggacgc ggcaaaaact caaccgtttt tcataaggaa atcagcaatc
    30301 gcaccgatcg ttttcccgcc tggtaatgaa tattggcgag aacattaaca ggcggaaaag
    30361 tggccccagc gtcctggcac cacgggcgag aataatcatg attcagttcg ggtaaaatat
    30421 gccgatcctg tcagtgaaca acgtggaaca accgcgaaca aataatttac aaaaaatgac
    30481 aaaaagccgg attgctccgg ctgtatgcgt gctcagtccc tgatcccttt cagaaattct
    30541 atcaatgcat ctatctgttc aggatttacc gccagcattt caccggacag agcacatctc
    30601 acaaaaccat gctgatcctt ttcaatcagt gcgcccgtct ccaggaatgc gcggtaatca
    30661 ttgatgctca tcgtctccat gttgtcagca tggtatttat cacgctgttt taatatctca
    30721 tcaaatttca tcggcatt
//
LOCUS       DAZFJF010000050        30182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFJF010000050 DAZFJF010000000
VERSION     DAZFJF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.050"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..710
                     /gene="yqaB"
                     /locus_tag="ACHKAU_002439"
     CDS             144..710
                     /gene="yqaB"
                     /locus_tag="ACHKAU_002439"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO5531490.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            707..1135
                     /gene="yqaA"
                     /locus_tag="ACHKAU_002440"
     CDS             707..1135
                     /gene="yqaA"
                     /locus_tag="ACHKAU_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO5531491.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1208..2764
                     /gene="gshA"
                     /locus_tag="ACHKAU_002441"
     CDS             1208..2764
                     /gene="gshA"
                     /locus_tag="ACHKAU_002441"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO5531492.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2914..3429
                     /gene="luxS"
                     /locus_tag="ACHKAU_002442"
     CDS             2914..3429
                     /gene="luxS"
                     /locus_tag="ACHKAU_002442"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO5531493.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHKAU_002443"
     CDS             complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHKAU_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO5531494.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHKAU_002444"
     CDS             complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHKAU_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO5531495.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVILDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHKAU_002445"
     CDS             complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHKAU_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO5531496.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHKAU_002446"
     CDS             complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHKAU_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO5531497.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHKAU_002447"
     CDS             complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHKAU_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO5531498.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8154..9338)
                     /locus_tag="ACHKAU_002448"
     CDS             complement(8154..9338)
                     /locus_tag="ACHKAU_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5531499.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHKAU_002449"
     CDS             complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHKAU_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO5531500.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHKAU_002450"
     CDS             complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHKAU_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO5531501.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHKAU_002451"
     CDS             complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHKAU_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO5531502.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHKAU_002452"
     CDS             complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHKAU_002452"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5531503.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHKAU_002453"
     CDS             complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHKAU_002453"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5531504.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHKAU_002454"
     CDS             complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHKAU_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO5531505.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHKAU_002455"
     CDS             complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHKAU_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO5531506.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17180..17509)
                     /gene="ygaM"
                     /locus_tag="ACHKAU_002456"
     CDS             complement(17180..17509)
                     /gene="ygaM"
                     /locus_tag="ACHKAU_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO5531507.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17661..18005
                     /gene="ygaC"
                     /locus_tag="ACHKAU_002457"
     CDS             17661..18005
                     /gene="ygaC"
                     /locus_tag="ACHKAU_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO5531508.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHKAU_002458"
     CDS             complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHKAU_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO5531509.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            19159..19563
                     /gene="stpA"
                     /locus_tag="ACHKAU_002459"
     CDS             19159..19563
                     /gene="stpA"
                     /locus_tag="ACHKAU_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO5531510.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(19610..20134)
                     /gene="ygaP"
                     /locus_tag="ACHKAU_002460"
     CDS             complement(19610..20134)
                     /gene="ygaP"
                     /locus_tag="ACHKAU_002460"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO5531511.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(20144..20443)
                     /gene="ygaV"
                     /locus_tag="ACHKAU_002461"
     CDS             complement(20144..20443)
                     /gene="ygaV"
                     /locus_tag="ACHKAU_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5531512.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            20626..20784
                     /gene="yqaE"
                     /locus_tag="ACHKAU_002462"
     CDS             20626..20784
                     /gene="yqaE"
                     /locus_tag="ACHKAU_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO5531513.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            20868..21317
                     /gene="kbp"
                     /locus_tag="ACHKAU_002463"
     CDS             20868..21317
                     /gene="kbp"
                     /locus_tag="ACHKAU_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO5531514.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(21318..21980)
                     /gene="csiR"
                     /locus_tag="ACHKAU_002464"
     CDS             complement(21318..21980)
                     /gene="csiR"
                     /locus_tag="ACHKAU_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO5531515.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(22001..23401)
                     /gene="gabP"
                     /locus_tag="ACHKAU_002465"
     CDS             complement(22001..23401)
                     /gene="gabP"
                     /locus_tag="ACHKAU_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO5531516.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            complement(23712..24992)
                     /gene="gabT"
                     /locus_tag="ACHKAU_002466"
     CDS             complement(23712..24992)
                     /gene="gabT"
                     /locus_tag="ACHKAU_002466"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO5531517.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            complement(25006..26454)
                     /gene="gabD"
                     /locus_tag="ACHKAU_002467"
     CDS             complement(25006..26454)
                     /gene="gabD"
                     /locus_tag="ACHKAU_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5531518.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            complement(26477..27745)
                     /gene="lhgO"
                     /locus_tag="ACHKAU_002468"
     CDS             complement(26477..27745)
                     /gene="lhgO"
                     /locus_tag="ACHKAU_002468"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO5531519.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            complement(27765..28742)
                     /gene="glaH"
                     /locus_tag="ACHKAU_002469"
     CDS             complement(27765..28742)
                     /gene="glaH"
                     /locus_tag="ACHKAU_002469"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO5531520.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            complement(29078..30182)
                     /locus_tag="ACHKAU_002470"
                     /pseudo
     CDS             complement(29078..30182)
                     /locus_tag="ACHKAU_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
BASE COUNT         7512 a         7721 c         7686 g         7263 t
ORIGIN      
        1 ctcccaaaaa tttccttcaa aaatatctca tttctaaata actactttat ccatttcctg
       61 aattttcttc tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa gtagcgtcat
      121 atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt tttgatatgg
      181 atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa gtattagggc
      241 actacggtct tcagtacgat gttcaggcaa tgattgcgct taatggatcg cccacctggc
      301 gtattgctca ggcaattatt gagctgaatc aggccgatct cgacccgcat gcgttagcgc
      361 gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg cttcctcttg
      421 ttgaagtggt gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg gggagtgaaa
      481 gcgccatcgc tgaggcattg ctggcgcacc tgggattacg ccgttatttt gacgccgtcg
      541 tcgctgctga tcacgtcaaa taccataaac ccgcgccaga cacatttttg ttgtgcgcgc
      601 agcgtatggg cgtgcaaccg acgcagtgtg tggtctttga agatgccgat ttcggtattc
      661 aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga gtgaagcgtt
      721 atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac ccggcaactc
      781 cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt tatccctggg ttttagtttt
      841 aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg ggcgtttctt
      901 tccattgcgt aaaacatcgc gctggcaaga gaaagctacc ggctggctga aacgctatgg
      961 tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt gtctgttagc
     1021 gggatggatg cgcatctcct gggggccagt aatctttttt ttgtgccttg gtaaagcgtt
     1081 acgctatgtt gcagttgcag cagcgaccgt tcagggcatg atgtggtggc actaattgta
     1141 ggcctgcaca tatggtcacc attacagtta tgctaattaa aacgattttg acaggcggga
     1201 ggtcaatttg atcccggacg tatcacaggc gctggcctgg ctggaaaaac atcctcaggc
     1261 gttaaagggg atacagcgtg ggctggagcg cgaaactttg cgtgttaatg ctgatggcac
     1321 actggcaaca acaggtcatc ctgaagcatt aggttccgca ctgacgcaca aatggattac
     1381 taccgatttt gcggaagcat tgctggaatt cattacacca gtggatggtg atattgaaca
     1441 tatgttgacc tttatgcgcg atctgcatcg ttatacggcg cgcaatatgg gcgatgagcg
     1501 gatgtggccg ttaagtatgc catgctacat cgcagaaggt caggacatcg aactggcaca
     1561 gtacggcact tctaacaccg gacgctttaa aacgctgtat cgtgaagggc tgaaaaatcg
     1621 ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactac aatttctctt tgccaatggc
     1681 attctggcaa gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga aaatttctgc
     1741 gggctatttc cgcgttatcc gcaattacta tcgtttcggt tgggtcattc cttatctgtt
     1801 tggtgcatct ccggcgattt gttcttcttt cctgcaagga aaaccaacgt cgctgccgtt
     1861 tgagaaaacc gagtgcggta tgtattacct gccgtatgcg acctctcttc gtttgagcga
     1921 tctcggctat accaataaat cgcaaagcaa tcttggtatt accttcaacg atctttacga
     1981 gtacgtagcg ggccttaaac aggcaatcaa aacgccatcg gaagagtacg cgaagattgg
     2041 tattgagaaa gacggtaaga ggctgcaaat caatagcaac gtgctgcaga ttgaaaacga
     2101 actgtacgcg ccgattcgtc caaaacgcgt tacccgcagc ggcgagtcac cttctgatgc
     2161 gctgttacgt ggcggcattg aatatattga agtgcgttcg ctggacatca acccgttctc
     2221 gccgattggt gtagatgaac agcaggtgcg attcctcgac ctgtttatgg tctggtgtgc
     2281 gctggctgat gcaccggaaa tgagcagtag cgaacttgcc tgtacgcgcg ttaactggaa
     2341 ccgggtgatc cttgaaggtc gcaaaccggg cctgacgctg ggtatcggct gcgaaaccgc
     2401 gcagttcccg ttactgcagg tgggtaaaga tctgttccgc gatctgaaac gcgtcgcgca
     2461 aacgctggat agcattaacg gcggcgaagc gtatcagaaa gtgtgtgatg aactggttgc
     2521 ctgcttcgat aatcccgatc tgactttctc tgcccgtatc ttaaggtcta tgattgatac
     2581 tggtattggc ggaacaggca aagcgtttgc agaagcctac cgtaatctgc tgcgtgaaga
     2641 gccgctggaa attctgcgcg aagaggattt tgtagccgag cgcgaggcat ctgaacgccg
     2701 tcagcaggaa atggaaaccg ctgataccga accgtttgcg gtgtggctgg aaaaacacgc
     2761 ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat ttcatctctg aattcaggga
     2821 tgatgataac aaatgcgcgt ctttcatata ctcagactcg cctgggaaga aagagttcag
     2881 aaaattttaa aaaaaattac cggaggtggc taaatgccgt tgttagatag cttcacagtc
     2941 gatcataccc ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa caccccgcat
     3001 ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca
     3061 gaaagaggga tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac
     3121 ggtaagggtg tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg
     3181 agtctgattg gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa
     3241 gacgtgctga aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc
     3301 acttaccaga tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt
     3361 gacgtacgca tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg
     3421 cacatctagt cagtaaacta tcttcacaat taattgaaaa aagccagttc aaatgaactg
     3481 gcttagttgt acttagtgcg caccgcctcc gccgccacct gcgccaaatg gcggtttagc
     3541 aaaccacacc agccccagca gtacgaggaa tattccggct gacatccaga agatctcatt
     3601 ggcggaaata atcagcccct gattggtgat ctgctgggca atccagcctg atgcctgctg
     3661 ttgcgtcatc ccaagccctt ccagttgact gtacatcgcc tgggcattcg ggttgaacgg
     3721 gtttaccgac tcagtcaact gcgcatggtg catcgactcg cggttggtcc acatggtcgt
     3781 ggttatcgac gtgccgatag accccgccag cgttcgcgta aagttagaga ggctcgatgc
     3841 cgccgccagt cgttccggtg gcaaaccaga cagcgtaatg gtggtcagcg gcataaagaa
     3901 gcaggccacc gcaaacccct ggataaactg cggccaggcc gacgcgccaa aatccatacc
     3961 tggttcaaag gtataggcac gccagtagaa gcagacggca tacataataa agctgaaggt
     4021 taccagccgc cgcatatcca gtttatgcgc gaagcggccg ataatcggcg acaggatcac
     4081 cggaataatc cctaccggcg cagaggccaa acctgcccag gtcgccgtgt aaccgtagac
     4141 ctcctgcaac aactgcggca gcagaacaat agcgccgaag tagagcatat acgcgaggct
     4201 gatacacaag cagccgatgg tgaagttgcg cgacttaaac aacgacagat cgactatcgg
     4261 gttatcgtcg gtcagctccc agacaatcag gaagcagata gccaccaccg ccaccacggt
     4321 aaggatgata atttcctgtg atgaaaacca atccagctct ttaccgcggt caagcataat
     4381 ctgcaggctg ccgataccaa taaccagcag tgccagcccc acggcatcaa tccgccgccg
     4441 ttcggtgcgg gtttcacgtc cgcgcagagt ttgcagtgtc atcaacacca ccgccacgcc
     4501 aatcggcacg ttgatgaaga atatccagcc ccagtggtaa ttatcgctga tataaccgcc
     4561 gaggatcggg ccgcaaattg gcgcgacaat caccgtcatc gaccacaacg ccagcgcgat
     4621 cgagcgtttg gctggcgggt agttattcag caatagactt tgcgaaagcg ggatcaacgg
     4681 cccggcgaca atcccctgaa tcacgcggaa gaagatcagc atattcaggc tgctggagac
     4741 accacacgcc cacgacgcaa tagcaaaggc gatggtggac caaaggaaca gtttcacttc
     4801 cccgacgcgc tttgccagcc agccggtaag cgggatcgag atggcattcg ccaccccgaa
     4861 agaagtgatt acccacgttc cctggctgag cgatgagccc agattcccgg cgatagtggg
     4921 gatcgccacg ttagcaatgg tggagtccag cacctgcatg aatgtcgcca gtgacagcgc
     4981 aatcgtcata atgaccagtt gcgcgccttc cagcggtttt tgctgttgca tcacacgcac
     5041 ctctggatta gccagcgtta gcttttacga tatcgtcgat cagtttattg acaggtgcca
     5101 ggctgatttc acgcgcggta cttaccgcga ccggtgttga acgtacttta tttgccagta
     5161 cctgaccgtc acggttagtg gtattgacgc tcaccagcgt ggacaaacca atgcgcagcg
     5221 ggtattgctc cagctgtttc tggtccagtt cgatacgcac aggtagacgc tgaacgactt
     5281 tgatccagtt accggtcgca ttttgcgccg gaagcagtga gaatgcgcta cctgtgccca
     5341 tatccagacc aaccacttta ccggtgtatt tcacatcatc gccgtaaata tccgtggtga
     5401 tagtgaccgg ctgaccgata cgcatattgg caatctgcgt ctctttaaag ttggcatcca
     5461 cccacatatt ggtggctgga acgaccgcca tcagcggcgt cgttgggcta atttgcgccc
     5521 caggctgtac cgcgcggcgg gagacataac cggtcatcgg actgacaata cgggtacgct
     5581 ccagcgccag ccaggcgtta cgtacttcgg tggcagcctg ttgcacggca ggctgatctt
     5641 ccagtttagt ccccagaatc atcgcctgat tggcattgta ttgttgaata gcgacgtcca
     5701 gttgcgcctg ggcactggtg acggcgtcgc gggcgtgttg cagctcttcg cgaccaatca
     5761 ggttggcatt gcccagcggc acacggcggt tgtagtcgct ttgtgctttc gcgagggcga
     5821 ttttttgcac ctcaatattc gcctgcaact gtttgctgtt aatcatcagc tggtgggttt
     5881 ggcgaacgct ggaagccagt gcagttttgg ctttttcaaa cgcctggcga gcatctgtcg
     5941 ggtcgagaat gaccagaaca tcgccttctt ttacaaaatc ggtgttatcg gcccagactt
     6001 tcgtcacgct gccagacacc tgagacataa tttgaatctg attccctgcc acgtatgcgt
     6061 catcggtttc ttcgaaatga cgcagtacca aaaaccaata aatccctatc gctacggcaa
     6121 taattataaa gagcaaggtg agaaggagga gcagacgctt acgtttgccg ctcttcttta
     6181 ccggttgctg cggggtttga gtctccgcat ttgcgctcat attgttctcc acgatcttct
     6241 taactcatcg gctgagccga gctgcttgcc aaaaaggcca gcatgttgcg atgctggccg
     6301 gtcatttttt cttttataaa tctggatttt tgagcgagat gacgcgttag ctcatcgctt
     6361 cgagaaccac accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt
     6421 gctcgagctg atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat
     6481 gctgcggtgg taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca
     6541 ggcagcggcg atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat
     6601 cggcaatacg cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct
     6661 gaatactgtg gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa
     6721 tcccctgagc cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc
     6781 aaagacgagt cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt
     6841 ttagcatttg ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc
     6901 agccgatata gtaacgacag tgacaaataa agtaaatgta ttgttttaga aaaatgattc
     6961 ttgtgggtta tataatcgcc atcactttcc aggcgagccc ataggccagc gcactaagca
     7021 gtgttgggat gataatgctg cgtgttttat agaaactggc acccagtacc gcgaagccga
     7081 ccagcgtggg cacgaaacgg cgtgtatcgt gcatcacttc tggtgcggta gagacaacca
     7141 gcagagcgca tatcgaggcg atgccaatgg tgtcgagcaa aatacctacc gcgccacgtt
     7201 tggttgggcg ggcattaccc acacgcaggc gcagcggcaa atagcggaag caataattcg
     7261 ccacgccaac cagtaaccca agcagcagaa cctcatagct catcgggcgc tccttgccag
     7321 aatgcctgga ttaacgcagt gaggcagcca cagacaatgc ctgccagaat ggcgacggga
     7381 atagaaaata gcgttacgcc tgcaagggca ccaactaacg ctgcggtaac gcaaagagat
     7441 tgtttgcgct ggaaagaggc gagcaggaaa ctcataaaga gtgccggaag cataaaacct
     7501 aatgcagctt caacggcggg ataaccttgc agcaagccgc tgccggagaa tgcccctatt
     7561 accgtaccaa atacccacga tgaccatgaa ctgaaggcaa tgccgatcat ccagttctcg
     7621 ctccagcggc gattattgcg taccagtttt gcggttgcgg cggcaaaaac ctcatccgtc
     7681 aggccaaatg cccacagggc ggttttcgat ttttgcagac gctgaataat acggctacgc
     7741 agtgacgggc catacaacac atggcgaaca tccattgcca tgacggtcag tgcagcaacc
     7801 cacaaactac tcccggctgc cagcatcgcg gtaatgacga actggctcgc gcctgcataa
     7861 atgatgcagg agaaaaaaac gctttcgaga ggagagaatc ccagacgggt cgcattcaga
     7921 ccgaacgcaa aggccaccgg aatataacta ataacaatcg gtaaactgtc tttgcatcct
     7981 tccatgaagg tcgccgaacc aggagcaggc tgtggagtag ggctttccat agagtgtacg
     8041 cttaacgatt gttaatatta taaatagact gaatgaatat cttaacctta tcagactgat
     8101 gggcttctta acacccttat aagtgtaaag ccacgaaaac ggttgctgat tgcttaattt
     8161 gcggcttcct gacgatgaaa acctcgccac cagaccagta agttaaccag ggcaatcgtc
     8221 gcgccagcca gacaaacgcc agcccaaccg ccatgttgcc aggctgaggc tgaaattagc
     8281 gaaccggcgg caccgccaat aaagtagctg gtcatgtaac ctgcggtcag gcgattacgc
     8341 gcatcagggt gtattcgata aattaccgtc tggttagtga tatgcacgcc ctgtacggtg
     8401 agatccagca ccaggattcc gataatcaac gccagtacgg aagtgtgacc aaaccagatc
     8461 gccagccatg aaagtaatag cagcagcaga ccgaaagttg tggtgtggtg cgatttaccc
     8521 ttatcggcaa aaccgcccgc cggacgagcg cccaacgcgc cggcagctcc cgcaagtcca
     8581 aacagaccaa tgacaccatc gctgtagtta aaaggtggag cggcaagcaa aaaggccatt
     8641 gaggtccaga gaatgctgaa gttggcaaag gtcaggcagc caagcaatgc gcgggtgcgc
     8701 aggatcttat cgttgataaa catgctgaaa acggaaccca acaactgtgg gtagttgagg
     8761 tgggtttctg atttcatttg tggcagacca cgccataatg ctagcgccat cagagccatt
     8821 aacaccgagg caacccaaaa gacggtgcgc cagccgccga ggtttgccag taacccggca
     8881 acggttcgtg ccagcaatat ccccaacagc agaccgctca taatggtgcc aaccactttg
     8941 ccgcgtttat ccggtgaagc cagcgtcgct gccagcggaa ccagaatttg tgccacgaca
     9001 gagaataaac cggttaatgc cgtaccgagg agcatcatcg ccagcgactg actgctggcg
     9061 gtaatcaaca taccgcccgc tgccagtaag gtcatcgaga caatcaggcg gcggcgttca
     9121 aacatatcac cgaggggaac aagaaacagc agacctgctg catagcccaa ttgcgcggcg
     9181 gtaacaataa agcctgccga actggcggaa agggaaaagt tacgcgcgat ggtgtcgagt
     9241 agcggctggg cgtaatagtt actggcgacc gccagaccgg tggcgataga catcagcacg
     9301 atcagcgccg ggctaagctc atgattaggt ttagtcatta tttttgttca tcaggtgatg
     9361 attaagctgg aaatcaataa tagcggatgt tgatgaatat gggatgaaag atagcttgtt
     9421 gaaatgtgcg atgtggtttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt
     9481 gtacagagcg gcggatgagg caaaaacgcc ttatccgccc gaataaaaat tacttctgcg
     9541 ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg atccagccat
     9601 caacatggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg
     9661 cgttaatatc tgccactggc aactgcataa tggcaaacag tttcgctgct gccgggtttt
     9721 tctcggccca ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg
     9781 caccattcgg cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct
     9841 gcaaccagac cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg
     9901 tgtagtaaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgccg
     9961 cgtagttccc ctgattatgc gtcacggtat gggtcagttc atacgcggca agctggtggt
    10021 tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct tttccgtcgc
    10081 cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt
    10141 tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacaaata
    10201 ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagtg
    10261 gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg tagccaacgt
    10321 tgtaatccac ttcgctgggt ttgttgacgg tataacctaa tttctccagc gcacgactga
    10381 ccagcagcgt ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc
    10441 ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg
    10501 caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg
    10561 tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca acagggccag
    10621 tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc
    10681 gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca agccccatat
    10741 ccagacgacc gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg
    10801 caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc ataatggtcg
    10861 gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc
    10921 gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc agacggataa
    10981 tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg ataccaaata
    11041 gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg
    11101 gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccagc ggcaaaccaa
    11161 tgacgataca gaacagcagg gcggttaaca ccagcgccag ggtaaccatg gcctgcgacc
    11221 aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc
    11281 cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg ggcataccca
    11341 gcagcaattg ctggaaaccg ttgagaatgt aatcaaccgg aacgcgcacg ccctggaaga
    11401 cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga
    11461 tcagcgtttt atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac
    11521 tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta ccccaggcgt
    11581 ctgcggattg cacggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag
    11641 ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg
    11701 acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag
    11761 agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca
    11821 tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggaaac tgcgccgaca
    11881 aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc
    11941 agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg
    12001 gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca
    12061 taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca
    12121 ttttgcataa tggcaattcg gtcgccaata cgcatagcct catcgagatc gtgggaaata
    12181 aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg
    12241 cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc cggattaatc
    12301 gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag
    12361 ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt
    12421 tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag cacggtcata
    12481 tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg
    12541 agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg
    12601 ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc
    12661 atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat
    12721 agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata tttgaacgct
    12781 cgctgtggat gctcgccaaa tattttataa agatttttaa tttctaattt aattgccatg
    12841 caatagaaag attcctttat ttgtttatgt cgatatatta cccaatggaa atagtcactt
    12901 ttttctaccc taacatactg agaatctgag gcaacccctg agggcaaatg tggcatgaat
    12961 attgatgcga gcaaatcgcc ggaatttccc gtgatataag ggctgagagc aaatcgagaa
    13021 aagttgatat tttttgtcat agatagcgaa aaaacgcctg aatattgtag tttcaggcga
    13081 ataatgcaac agagtatcag tgtagatcac tacagaatat ttgcgtgata aaaagctatt
    13141 tggcgggaat tatttccctg ctgcgggtag tgatattttt gaaaataaca ccctcagaaa
    13201 ttccagtctt catcttctgt ttcaaccgct ttccccatca cataagagga gcctgaaccg
    13261 gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg cggcgaggat tgccggattc
    13321 acttccgcca tttctgcggg aaataacggt tcgtagccca gattcatcaa agccttattg
    13381 gcgttgtaac agagaaacgc tttcacatcg tcagcccacg gggtttcggc gtacagctca
    13441 tcggtgtatt gcaactcgtt gtcgtagagt tccagcaaca aatcgaaggc gaaactcttc
    13501 aactcttcac gttgtcccag agatatcttt tccatgtttt tctgatattt atagcctatg
    13561 tagtaaccgt ggactgcttc atcgcggata atcagacgga tcagatctgc ggtattggtc
    13621 agctttccgc ggctggaaaa atacatcggc agccagaaac cggaatagaa caaaaaagat
    13681 tcaagaaaca cactggcgat tttctttttc agcggatcat caccgcgata atgttgctga
    13741 ataatctgag cttttcgctg caacggtgcg ttttcttcac tccaggcgta ggcggcatcg
    13801 acatctttgg tctgacacag cgtcgagaaa atcgaactgt aagagcgggc atgaaccgct
    13861 tccataaagc tgatattcga taatactgcc tcttcatgag gcgtgagcgc atcgggcatc
    13921 agcgaaggcg cgccgataac attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg
    13981 cgcattgtca gttgttgttc tacgacactt aatgtctgcc atgcaggtat atcgttcgac
    14041 agtggcacct tttctggtag ccagaaattg ctggtcaggc gattccacac ctccagatct
    14101 ttatcgtcag atatcttgtt ccagttgatg gcgctgatac gtgagagttt catagatatt
    14161 ccttaaagtg cacaggagac gcagccttca atttcagtgc cttccagcgc catctgacgc
    14221 aggcgaatgt aatagagcgt tttgatacct ttgcgccagg cgtaaatctg cgctttgttg
    14281 atatcgcgag tggtggcggt atcggggaaa aacagcgtca gcgacaaacc ctgatcgaca
    14341 tggcgagtcg cttccgcgta ggtgtcgatg atcttttctg cgccgatttc gtaagcatcc
    14401 tgatacagcg ccagattctc gttagtcata aacggggcag ggtagtaaac gcgtcctgtt
    14461 ttgccctctt tgcgtatctc tactttcgcc acaatcggat gaatactcga cgtagcatgg
    14521 ttgatataag agatagaacc ggttggcggc accgcctgga gattctggtt gtatatgccg
    14581 tagcgcatca cgtcgtcgcg cagttgcgcc cacatctcac gggtaggtaa cgtaataccg
    14641 ctgcgggcaa acagttcgcc aactttcgcc gttttcggct gccagttacc ttgcagatat
    14701 tggctgaaat attcaccact ggcatagcgt gactgtttga acccggcgaa ggtttcaccg
    14761 cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggt atagaaatag
    14821 agattggtga aatccagggc ttccggcgaa ccataagcga tgccttccct cgccagataa
    14881 ccatgtaaat tcatctgccc cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg
    14941 gacggcacgc tgcggatatg actcatatct gacaccgccg ttaaaccgcg tacggcagtc
    15001 tctaccgtgc gggcaaagtt gggggaatcc atggtgtggg caatattcaa cgaacctaaa
    15061 ttgcaggaaa tatcatggcc tgtacgggca tagtcgagat tctcgtcata ctctgaggcg
    15121 ctgttaacct gcaaaatttc tgagcagaga ttactcatat ttatgcgccc ggcgataggg
    15181 ttagcacggt ttaccgtgtc ttcatacatg atgtagggat agccggactc aaactggatt
    15241 tctgccagtc gctggaagaa atcacgggcg ttgaggtatt ttttgcggat gcgttcatcg
    15301 gcaaccagtt cgtcatagtg ttcgctaatg gcgatatcgg caaacggctt gccataaact
    15361 cgctcaacgt catagggcga aaacagcgcc atctgcgcat tctcttttgc cagatggaaa
    15421 gtgatatccg ggatcaccac gccaagcgac agtgttttaa tgcggatttt ttcgtcggca
    15481 ttttcccgtt tcgtgtcgag aaaacgcaga atatcgggat gatgagcatg taaatagact
    15541 gcaccagccc cctgacgcgc gccgagttgg ttgggatagg aaaatgcgtc ttccagcatt
    15601 ttcatcaccg gaattacgcc agaagattga ttttcaatac gtttaattgg cgcgcccgct
    15661 tcccgcagat tcgacagcaa aaatgccacg ccgccgccgc gtttcgacag ttgcagtgcg
    15721 gaatttaccg cccggccaat cgactccata ttgtcttcaa tacgcagcag aaaacaggaa
    15781 accagttcgc cgcgctgctg cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag
    15841 cgtcctgaca gcatttcatc ggtcagttgc aacgccagcg tcgcatcgcc ttgtgccagc
    15901 gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca gataacgttt accgtcgaat
    15961 gtcttcaacg tatagctggt gtaaaacttc catgccccga ggaatgtctg gaaacgaaaa
    16021 ccgctggtgt gtgcgtgggc aaacagcgta atgacaaagt cgcgagagta gcgattaaga
    16081 acgctttcat catagtaacc ttcgttgacc agccagttca ggcgctgctg ctggctactg
    16141 aaggtcacac tgttcggacg cacatgcgtc gcaataaagg cgtcaacggc ctggcggtct
    16201 ttatcgaact gaatgcgacc tgcgctatcg tagaggttaa gcatcgcatt cagcgcgtgg
    16261 taatccatcg tttcctgcgt caggcattct gcggttgtcg ttgccaaaat tcggttactc
    16321 ctttacgaac gttttcgata tcgctttgcg tacccatgag ttcaaaacgg tacagccacg
    16381 gcacgccgca tttccgggca atcacatctc cggcgcggcc atacgcctca ccaaagttgc
    16441 gattaccaga agcaataacg ccgcgaagca acgcccggtt gtgctcgtcg tttaaaaagc
    16501 gaattacctg tcgtggcacc gcgccagccg taccgccgcc gccgtaagag ggcacgatca
    16561 ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg
    16621 gcagacctaa acgttcgata aaacgctgcg tgttttcgga gctgctggag aagtagacga
    16681 gctggctcat gcactggccg cgtgtggcgc tggatgcaga cggttaatca tgtccggacg
    16741 gaaaccagac cagctaagat cgccagcaat cactaccggc aactgacgaa agccctgagc
    16801 acgcaacgct tccgctgctt caggaacgcg atcgacatta atcatttcaa aatcaaagcc
    16861 ccggttttcc atcgcccgtt tggtggcgtg gcactgaacg caatcgttac gagtgtaaat
    16921 agtaatgcgc atgattcgta tttccgttta aaatgaagat acggcgcgat gatacgcgtc
    16981 gggttgtctc tctgttgata cagagatact agatgtagtt gaaaaaagat tcaaccacac
    17041 aatatatagc aaatagtggt cgaaattacc ctggatatga gcgtgacggg gtagggggat
    17101 ttttgtgatt caccaggcaa aaagaaaccc cgaagccagg cttcggggtc aaagacgcgt
    17161 atttattatc atttttgcac tacgatttgc gcatgcttaa cagtgcgccg ataaaaatac
    17221 ctaccgcagc tgctgtaccc acgctacacc agggtctttc acgaacaaaa gaatccgcgc
    17281 agccaacggc atcgcgtgcg gcttgctgga cgcgagtacg accatgcatt cgcgccctgg
    17341 tttctttcag caatgcctga gctttactgc gtgcggcttc agcttcccct ttggcgtcgc
    17401 tgccccagga cttcaataca gattcgaggc tgtcagctaa ttgattgaca tcattctgga
    17461 tatcctgcac gccatcatca acgtcgttgc ggttcggtcg gttaaacata tgatccccca
    17521 ttagaattcg atgtaatttt cagtgtagac cataattttc ttaactgagg ttcatcgcgg
    17581 ctttatggac ttttctgaaa gcgttgtgaa tgcgcaatca ggtaaggtag gcccgcggtt
    17641 gataataacg aggatcaaat atgtatttac gaccagacga ggtggcgcgc gtacttgaaa
    17701 aagtcggttt tactgtcgat gtggtaaccc agaaagcgta tggttaccgc cgtggggaaa
    17761 attatgtcta tgttaatcgc gaagcgcgaa tgggacgtac cgctctggta attcatccaa
    17821 cattaaaaga acgcagttca acactggctg aacccgcttc cgatattaaa acttgcgatc
    17881 attaccagca atttccgctc tatttagcag gcgagcgaca cgagcattac ggtatcccgc
    17941 atggctttag ttcgcgtgtt gcgcttgaac gttatttgaa tggtttattt ggcgaagcca
    18001 gttaaagacg cgactggcga tgccagtcgc gaaaagaaga gtcaggcttt tacctgctgg
    18061 taacggctga ctttaaacag tcggcggcaa taatcgagga agtagccata aaccgccccc
    18121 atcaacatcg aaacaacgat gtttgaactg accgccgcca taatctgatg ccagtctgcg
    18181 cccaccacta acaagatcgc cacatacacc ggtgactgga acgtcacata agccaggata
    18241 tcagccagat tttttatcca gcccgacggg ctaactttgc gtgccgcgcg cataaacaga
    18301 tcgcggtaca taccgtatgg ccatgcaatt aagatgttcg ccggaatcgc taccaatctg
    18361 gaataaaaag actgttcgaa gctcattccg gagaggaaaa cttcaataca catgttcacg
    18421 acagaacagt aaacaaccat cgcgaacgta tctgcaactg catgacgcaa gcgtgactgc
    18481 ggtgagaaca tgctaatgct ccttaataaa agcgtaatgg gatgttaatg gagatgaaag
    18541 cagtggatct cattgccatc tgcattttcg catagcgtat atcgctggct atgactattc
    18601 aattagtgaa aaatttttat ttagtcagtt tttgtagata aaatattccg taacggctcg
    18661 ttttttgtat actgtggatt tttacatgcc gatatgattt attttcaatt aaatcaattg
    18721 gatatgacct tgcctttaat ttcactaatg aaagtttggg ggtgaggtaa cgctataagc
    18781 gtagagaatt cggtgtatgt cgtgaccggg tcatttacag cattttttat gccagaaaat
    18841 gtacattaac agcagaaagc accagtgagg ggtgttttct tttattctgt tatattgcat
    18901 ttttattcac ttcggttttg tcaggcttgc ggaattagcg agcagagagc accactctgt
    18961 tcaccaataa tctaaattac tcatcaaaat ggctaatata taatcttgaa attattctga
    19021 gagtttcaga aaataacagc tgaaataatc tcgcgcagga ctgtaaatag attaaatttt
    19081 gtggaaatat aataagtgat cgcttacact acgcgacgaa atactttttt gttttggcgt
    19141 taaaaggttt tctttattat gtccgtaatg ttacaaagtt taaataacat tcgcactctc
    19201 cgtgcgatgg ctcgcgaatt ctccattgac gttcttgaag aaatgctcga aaaattcagg
    19261 gttgtcacta aagaaagacg tgaagaagaa gaacagcagc agcgtgaact ggctgagcgt
    19321 caggaaaaaa ttagcacctg gctggagctg atgaaggctg acggaattaa cccggaagag
    19381 ttattaggta atagctctgc tgctgcacca cgcgctggta aaaaacgcca gccgcgtccg
    19441 gcgaaatata aattcaccga tgttaacggt gaaactaaaa cctggaccgg tcagggccgt
    19501 acaccgaagc caattgctca ggcgctggca gaaggtaaat ctctcgacga tttcctgatc
    19561 taattactga ggccggatgt cgctgctagg gcgcgtccgg cgtcgcttct caagttcgtt
    19621 ggttccacgg catcttatct aacaaccttg ccattccaca aaaaccgctg attcctgcaa
    19681 acagtaaccc ggcaccaaca aagccactta ataagaagaa accgctattt acggtataac
    19741 ccagtacaac gccgattaat attaaaccac ccgcagcgat ctgcacctgg cgcattaacg
    19801 gcaagggttg agatttattt accgctactg gcaatccagc tcttttccag ccatcaatcc
    19861 catcttcgag taaaaatatt tctgcggggg cggcaatcgc tgctaattta tcggcgttat
    19921 tactggtgcg tttacctgcc tggcagtgga atataatttg ctcgcgacgt aatttaggtg
    19981 gaagacctga ctgttccagc acggataatg gagccagatc tgcttcagga atatgttcac
    20041 gaagatattc atcagcgtca cgaatatcga ttaactttgc gccgcgtgcg attaattctt
    20101 gtgcatcatg cggcgaaatg gttgtcaaag ccatagtgac tccttacgga caatagacat
    20161 ttttcagggt ggcgataatg gcatttaccg cctcattttt aatggaatat agaatgcgtt
    20221 gggcatcccg ttggctgtcg ataagccctt cgtcccgcat acgagcgaga tgctgtgatg
    20281 tcgcagaggc actcagtccg gtaatgcgcg tcagctctcc cgcgctggtg ccgggggaac
    20341 cgctaagcat gcacagaatc agcaatcgtt taggatggct cattgctttc aataaggccg
    20401 ctgcctgttc ggcactggcc tgtaattgcg cgagttcagt catatttatt taagtatttt
    20461 ctaaattaag taaactctaa actaaaaatg caacatatac cagcctcagc agcgtaaatg
    20521 agagtaaaag cgtaagctga aactggcagg ctccgctaaa attactacgc ttaagagata
    20581 aaatctcttt ttaaacaatg agtaattttc ttatagggag tacatatggg tttctggaga
    20641 atcgtcatca ccatcattct gccgccgctc ggcgtgctgc tcggtaaagg gttcggttgg
    20701 gcgttcatta ttaatattct gctgacactg cttgggtata tccccggttt gattcacgcg
    20761 ttctgggtgc aaacccgcga ttaaacctgt gctcaccccc gcggcgttaa cgcgccgcgt
    20821 tcctctgcta cactttctga gtgtttccgt taacgaatga ggtagctatg ggtctgttca
    20881 attttgtgaa agatgccgga gaaaaactct gggacgcggt tacaggtcag cacgataaag
    20941 acgatcaggc gaagaaggtg caggagcatc tgagcaaaac cggtatacct gatgccgata
    21001 aagtgaacat tcaaattgcc gacggcaaag cgacggtcac tggtgacggc ctgagtcagg
    21061 aggcgaagga gaaaatcctt gttgcggttg ggaatatttc cggtattgcc agtgtcgatg
    21121 atcaggtgaa aacggcgaca ccagccactg ccagccagtt ttataccgtt aagtctggcg
    21181 acactctgag tgccatttcc aaacaggtct acggtaacgc taatctgtac aataaaatct
    21241 tcgaagcgaa taaaccgatg ctaaaaagcc cggataaaat ttatccgggg caagtgttgc
    21301 gtattccgga agagtagtta attgccagcc atcgcctgct ggataatggg aattggcgtc
    21361 agtaaatgct ggcgcattaa ctcactggcg cgcgcggtat ctcgcgccag taccgccgca
    21421 gtcagggtct ggtgctgatc gtgtttatcc tccagcattt ctaccgaaag caccgttcgc
    21481 cgcagccaga taaatcgata gcgcgccgcc agatcaaaca accgttcacg catttgcagc
    21541 aaatagtgag aaccacagcc cgccacaatt gctgtatgaa acgcctgatg acgcagatcc
    21601 cactcatcaa gcattttctc gctggcgtca caggcttcca gcttactgag cagatgcgct
    21661 tttgcgagaa cgtctgcctc ccattcatcg ccaccgcggg caatcgccag actgaccagc
    21721 atcgcttcca tattggcgcg ggcgtcgaaa atatcgagca gctcctgctc tgacatagac
    21781 gccacccgat accctttttg attcaccacc gtgaccagcc gttccgccac cagttgagaa
    21841 agtgcttccc gcaacggccc aacgccaagt gcataacgcg atgtcagcaa actcatccgt
    21901 aatttttcat ccggttgaaa attaccgcga ataatatcgt tcttcagcca gcgatagcca
    21961 tccagagacg taatggtcat ggtgtgactc ctgatcatta tcagcgcgta ttatgaacgg
    22021 gtgttttttg ccacaatacc agctttttcc agcgagccat aattggcacg gtacagataa
    22081 tccctatcgc taataagccg gtggagatca cttctaactg ttgcgccgga cggaatagca
    22141 tcactaccaa cacaaaggta ataaagccga tgaccagcca ggtgagccag ggataaagcc
    22201 acatgcgcaa gcgaatttcg cttccttctg ctcgcagaat tttgcgcatc cgcaactgtg
    22261 aaacggcgat gactaaataa accagcaggg cgatagcgcc ggagctgtct atcaggaatt
    22321 taaacacttt cgcaggtgcg taatagttca ccaccaccgt taaaaatgcc gctccggtgg
    22381 agagtaacac cgccacatac ggggttttac tgcggttgat tttgcccatt agcgcgggcg
    22441 catcaccgcg acggcttaag gagtagagca tccttgacgc ggtatacagc gccgagttca
    22501 gacagctggt tacggaaagt aatatcacgc agtccatgat taatttcgca tggggaatat
    22561 tgagcaattc cagaaccgag cgataagaac caacggcttt cagccccggc atattccacg
    22621 gtattaacgc cactacgaca aaaatagagc acaaatagaa gatagaaata cgccagataa
    22681 ccgagttggt agcgcggaca atatgttttt ccggcgtgtc ggattccgcg gcggcaatgg
    22741 tgacaatttc tgcgcccata aacgagaaca tggtgatcaa catcgcgctt aataccgcac
    22801 cgaagccgtt gggcataaag ccgccgctat cccacaatct tgagatcccg ctcacttcgg
    22861 cataagggta aaaaccgcta attgcgactg caccaaggaa aataaaggcc aggatagcga
    22921 tgactttgca cagcgccagc cagaactcaa attcgccgta gtttttaacg cttaataaat
    22981 tactgccagt taaggcgagg gtaatgacga gggaaaataa ccagatggga atgcctggaa
    23041 cccacgagtg caggatcata gcggcgatgt tggcttccag cgggataacc agtacccaaa
    23101 accaccagta cagccagccg atggtataac ccgcccagcg gccaatggct ttatcggcat
    23161 aggtggaaaa cgaaccggta tcgggcgtgg caactgccat ttccgccaac atccgcataa
    23221 tcataaccac cagtaatccg gcgaacagat aggccagtaa taccgccggg cccgcttcgg
    23281 cgatggcgac gctggaaccg acaaacagac ttgcgccgat aacaccggca atagacaaca
    23341 tggtgacgtg gcgtgacttc agcccgccgc ctaactcatg tggttgcgat gattgcccca
    23401 tcctgaatcc tctcgaaagt gtgtcacttg ggagagcgcc ccgacatttc ccgcgtcggg
    23461 tcacgcgttt aattgagacg gtcaacaaaa tcttaaaaac gaaagcggga gagttttctc
    23521 cctctccctt agggagaggg ccggggtgag ggtgtagccg cgtgattacc cctcacccta
    23581 accctctccc cagaggagcg aggggacagt ccgcagtcgt ttttaacttt gtcgatcgcc
    23641 tcatcgttat tattttttac tgcatcgtta tcattccctt agcaacgttt aactcttaca
    23701 aacggacata gctactgctt cgcctcagca aaacactggc tgatgatctc cagaccctga
    23761 cggatctgag cgtcttcaat ggtgagcggt acaaggatgc gcagcacgtt gtaatacggg
    23821 ccgcaggaga gaagaatcag gcccttatcg cgggcgcgtg ccacgatctc ggcggtgagt
    23881 tttgcgtccg gtttgctgtg atcgccatct tcaaacagct cgatggcaat catcgctccc
    23941 agcccgcgta cgtcgccgat ctccgggtgt ttttcggcta tcgccaacaa tccgtctttt
    24001 agcttctgcc ccagatcgtt ggctttttgc agcagatttt cctgctcaaa caccttcaac
    24061 acttccagcg ccgccacgca ggcaatcggg ttaccggcat aggtgccgcc cagaccgcct
    24121 ggagcgacgg catccatcac ttccgcgcgc ccggtgacgc ccgccagcgg gaagccgccc
    24181 gcgatcgatt tcgcaaaggt agtgagatcc ggcgcaacgc ccatctgctc catcgcaaac
    24241 agcgtgccgg tacgccccgc gccgctctgc acttcatcgg caatcagcat gatcccgtgc
    24301 tcgtcacaca gcgcgcgtaa acgctgcata aaggctggcg acgcggcgta gaaaccgcct
    24361 tcgccctgaa ccggctcaat cacgatggcg gcgatatctt ccggcgcggc atcatttttg
    24421 aagatccggt ggatgctggc gatagcgtca tcctcgctta tgccgtgcag cgggcaagga
    24481 taaagcgcgc gataaacatg acccggcatc agccccatgc ccgcagagta cggattcacc
    24541 ttgccggtca gcgccagcgt gtaatgcgtg cgcccgtgat acgcgccgct aaaagcgatg
    24601 gtgccgctac gtttggtggc ggcgcgggcg atttttaccg cgttttccac cgcttcggaa
    24661 ccggtcgtaa ccagcagcgt tttcttggcg aaatcgcccg gcaccttctg attcataatc
    24721 tcgcacagct ccagatacgg ctcgtaagcc agcacctgga agcaggtgtg cgacagtttt
    24781 ttcaactgcg cttccaccgc ggccaccacc ttcggatgca ggtgcccggt attgagcacc
    24841 gcaatcccgc ccgcgaaatc aagatactca cggccttcaa cgtcccacac ccggcagttt
    24901 tccgcgcggt cagcgaaaat cgggtgaatt tgcccaacgc cacggggaat cgcctgactg
    24961 cggcgctgca ttaactcttt attgctgttc attcgcattc tccagttaaa gaccgatgca
    25021 catatatttg atttctaagt aatcttcgat gccatacttc gaaccttcac gacccagacc
    25081 cgaggctttg atgccgccga acggggccac ttcattggaa ataatgccgg tattgatgcc
    25141 gacgatgccg tactccagcg cttcgcccac gcggaagacg cggcttaaat cacgggcgta
    25201 gaaataggcg gcaaggccaa actcggtgtc attggcttgc gcaatcacat cggcttcatc
    25261 tttaaagcgg aacagcgggg cgagggggcc gaacgtctct tctttcgaca ctttggcgtt
    25321 ggccggcacg tccaccagaa tggtcggctg gaagaagttg ccgccgcgtt catccgcttt
    25381 accgccgcaa accacacgcg cacctttctc cagcgcatcg gcaatatgct cttccacttt
    25441 tgctaccgct ttttcatcga tcagcggccc gatggtgacg cctttatcca gcccgtcgcc
    25501 gatgtgcagt ttgctcaccg cctgctgcaa tttttcggca aagcggtcat acacgccgtc
    25561 ctgcacgtat aaacggttgg cgcagacgca ggtttgcccg gcgttgcgga atttcgaggc
    25621 cagcgcgcct tccacggctt tatcgagatc ggcatcgtca aagacgataa acggcgcgtt
    25681 gccgcccagc tccagcgaca cttttttgat gtctttcgcg cactgttcca ttaactggcg
    25741 gccaatttcg gtcgaaccgg taaacgacag tttgcgtacc atcgggttgc tggtcagttc
    25801 attaccgacc gcgcctgccg aaccggtgac cacgttaaac acgccagccg gaatgcccgc
    25861 gcgaatcgcc agctccgcca gcgccagcgc agagaacggt gtctgactgg cgggcttcag
    25921 caccatggtg cagcctgccg ccagcgccgg accggcttta cgggtaatca tcgccgccgg
    25981 gaagttccac ggcgtgatag ctgcggtaac gccaatcggc tgcttgataa caatcaggcg
    26041 tttatcggcc tgatgaccgg gaatggtgtc gccataaatg cgtttgcctt cttcggcaaa
    26101 ccactcaata aaagaggcgg cgtagctgat ttcacctttc gcttcggcca gtggtttgcc
    26161 ctgttcgagg gtcatcaggc gcgctaaatc gtcctgatgc tccatcatca aattgaacca
    26221 gttgcgcaga atgttggcgc gttctttggc ggtgagcgca cgccaggcgg gcagggcgcg
    26281 gttggcggcg tcgatagcgg cgcgggtttc atcagcgccc attttcggca cgctacccag
    26341 cttgtcgccg ttcgccggat tggtgacgtc gatgacttca ccattgttgg cgtccagcca
    26401 ttccccgtta atcaacgcct gctggcggaa taagttactg tcgttaagtt tcattgttac
    26461 gtcctgtttt caaaggttat tgattaaatg cggcgtgtaa ggcatccaca ctacgtgccg
    26521 ctcgcagcgt gcgtccgggg ttactctggc ttgccaacag cgtttgtacc ttgctgacaa
    26581 tatgcgcacc aataggaatt gctgatgtcg ctgccgggga gggcgcattg caggtgtgga
    26641 tcgtgcgtgg ggtggtgaca aacagaaaat cgtcaatcag cttgccgtcc ggcgatactg
    26701 cctgcgcccg cacaccggcg ggccagggct ggagatcgct taacgaaagc cggggacaat
    26761 acttttgcac caggcgcaga tagccgcttt tgcacagcga gtttttcatc tcgcccagtc
    26821 ctgagcgtag atggttttgc agcacccggc gaatccccga cgagcccaaa atctccagcg
    26881 tgtcgctaaa tgagaagtcg cgcttgcgat agccttcgcg tttgaaagcc agcaccgcgt
    26941 ttggcccgac ggtgacgctg ccatcgatca ttcgggtgag atgcacgccc aaaaatggca
    27001 ttgccgggtc gggaatgggg taaatcagat ggttaacaat ctggttatgc tccggcgcaa
    27061 ggcggaaata ctcgccacgg aacgggcaga taataaaacc cggttcgagg ccgagcattt
    27121 tcaccagccg gtcagccatc agcccggaac agctaatcag cgtcgatgct tcatattcgc
    27181 cgccctgacg ggttcggacg accacgccgt ttttatgctc actgagggcg ctgacttcgg
    27241 cgttataaat aatctcaccg cctctggcct ggaagatttt tgccattgcc gctgtgactt
    27301 cgcgatagct gacaatgccg ctggacggca caaaaatgcc gccgagcccg gtgatattcg
    27361 gttcgcgctc gcgcagttcc tcggcgttta accactcgcg ctcgataccg ttcgccgccg
    27421 tgcgttccca caacgcgcgc atccgttcca tttcgagatc ggacgtggcg accagcatct
    27481 tgccgcagtt gtcatagcga atgtcgtttt gatcgcaaaa ggctttggtg gcgcggtttc
    27541 ccgccaggca aaactgcgcc ttcaggctgc cgggcgtgta atagaccccg gcatggatca
    27601 cgccgctgtt gtggcccgtc tggtgacagg ccgggccgga ctctttttcc agcaacgcaa
    27661 tgcgggcatc cggataaaca tcaatcagtt gcatggcggt cgacatgccg atgatgccgc
    27721 cgccaataat cacaaaatca tacatccgct caattccttt gcgcttactg atgcgtctgg
    27781 tagtggtgag tggcgtaagc gaaatagcca cgctgacgca tcagttcacg gcgcagatcc
    27841 gggtgcggag taaagcggtc gcgaccgtgc agccagaaca ggttgtttat caacaggaat
    27901 ttgccaacgg gaacgggtac agaaagaatg cctttgctgg tttcaatggc atcggaaagc
    27961 tcgctcaacc acacgccttc ttcaaaatct tttggctgga cgaactggtc gatatagcgc
    28021 atcaccgggc gaccctgttg atcaacgtcg aacactggat ggaaaacatc tttgctgacg
    28081 tttttgctcg gcggcgcggc aaagcgcatc gggcgacgcg ccagtggatg gcggaaatag
    28141 tggtccagat gttcccagtc atcgagatgc agcagcaacg aatttccacc ctgcatgttt
    28201 tgctcatcga ttttcatcat cagcacgtaa tcggtgatct cttcgacata cgtgccgtcg
    28261 ttgtgcagtt ccatcacgcg gtgcggctga cgcagatagc tgtctgagtt atcgacattt
    28321 ttcaccacga aacgcgcgta atactgaccg ctcatcgcat cgaaatttga gcgaccaatc
    28381 agatgcgcta ccgccgttgc cagcttcacc atctcatctg cctgcgccac atcatcgata
    28441 cccaccgcat tgatcaacag cgcaccttca gcacggttta gcagggtctt caacagcaat
    28501 ggttgcagct gattcgcaca cagatcgtca agaattttgc ctacccgaaa acgcagaaac
    28561 gatttgtact ccagcgcctg cacgggccat tcggcaacct gttcgagaaa ctgtttggtc
    28621 gtctgttcgg tgaaggtgag ttccagcaga cgcggggatt gcgccgacgg gatgagagtg
    28681 aatccgctat agtcctggcc tgaatcgaca gcgttattgt gtacggcggt cagtgcattc
    28741 atcagaagcg atcctcttat gagatgtagg gtgacatggc gatgctcatt tcgtagccat
    28801 aatctaaaaa tatctacatt tctgaaaaat gcgcacaaaa gcgacatatt gttttcttat
    28861 tgtgatcaaa agcaacaaat tagtaacatt taacgtaggg tattaaggca aagtgagaca
    28921 ggaaatgaat aactaaagca acaggttagg gggagttaac attagagaac agattatctg
    28981 gtcgataaaa aacagtaaat ggcttcactg caaaatgacc gttagtaaac taaacatatt
    29041 atctgccccg ttttatcggg gcagatataa cattaaatta taaatagaga tagttcggcg
    29101 gttctcctgt tttccctaaa agaagagcgc tacgcccagg aatattgata tttagagttc
    29161 cgcctctaag cgtaaatact ttaccagggg ggataatttt tcgggtatta ttaataataa
    29221 tgttaacgct attactcccc atgtttttgt atattccagg ttgcaatttg ttaggccagg
    29281 taccttcaac ttcttcgttt cctttattta ttataactag tccatcatga ggagagtgat
    29341 taatgttcac agaatcccca cgactaaagg ccagaacgtt tttagttgca gatacaccct
    29401 caaatgcgct ccatattcct gctttatcta tatataatgc gtggaattca ttgtagagct
    29461 gctgataggt agagcaacct ttattaagct acgcctcacc gccgacacgc gtggacccat
    29521 cataattttt aatttcagtc gttgatttta atacttggcc atcccagtaa actaaaggcc
    29581 tgctgaacgg cagagcggct atatatgcca ttgctaattg ttcatcttgt tgatggttaa
    29641 atagaaagtc attgaaaaga ttaccattgt taggaaaatc gtggttaatg ttaatcaata
    29701 tccgtctttc tgatgggata cttgagattg tggaacccgg ttttgatgca agggtttgaa
    29761 gatctccacc aaatgaaaag gcttttatcg ttttcatgag caatgaaaaa tcaatggcgc
    29821 ttaaatcatc tctttcataa agcagtgatg acatttgttg ttcatccaac tgacaagtta
    29881 ccagatattc catgtaattc ataacggctt ttttatgata ttttgggtta aataagttag
    29941 tattgtgtaa ccgctcatta taatttttaa gcggtggggt tattgatctt tctatttgtt
    30001 catgttttga gtgttttgcc gcatcataac gtaaacctcg tacgccatat tttcgcattt
    30061 cacgaacgta ttgattttgc aacataataa cctggtcact tttgggtgtt gttttaggca
    30121 ttccatttaa acgcttttct tttaatgcat ggaggttttc ccattcatcc ttagagatgt
    30181 ta
//
LOCUS       DAZFJF010000051        30159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFJF010000051 DAZFJF010000000
VERSION     DAZFJF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.051"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..206
                     /locus_tag="ACHKAU_002471"
     ncRNA           75..206
                     /ncRNA_class="other"
                     /locus_tag="ACHKAU_002471"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(390..1067)
                     /gene="narI"
                     /locus_tag="ACHKAU_002472"
     CDS             complement(390..1067)
                     /gene="narI"
                     /locus_tag="ACHKAU_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5531521.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1067..1777)
                     /gene="narJ"
                     /locus_tag="ACHKAU_002473"
     CDS             complement(1067..1777)
                     /gene="narJ"
                     /locus_tag="ACHKAU_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5531522.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1774..3312)
                     /gene="narH"
                     /locus_tag="ACHKAU_002474"
     CDS             complement(1774..3312)
                     /gene="narH"
                     /locus_tag="ACHKAU_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5531523.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKVMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3309..7052)
                     /gene="narG"
                     /locus_tag="ACHKAU_002475"
     CDS             complement(3309..7052)
                     /gene="narG"
                     /locus_tag="ACHKAU_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO5531524.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7567..8958)
                     /gene="narK"
                     /locus_tag="ACHKAU_002476"
     CDS             complement(7567..8958)
                     /gene="narK"
                     /locus_tag="ACHKAU_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO5531525.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9297..11093
                     /gene="narX"
                     /locus_tag="ACHKAU_002477"
     CDS             9297..11093
                     /gene="narX"
                     /locus_tag="ACHKAU_002477"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO5531526.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            11086..11736
                     /gene="narL"
                     /locus_tag="ACHKAU_002478"
     CDS             11086..11736
                     /gene="narL"
                     /locus_tag="ACHKAU_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO5531527.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11737..13131)
                     /gene="ychO"
                     /locus_tag="ACHKAU_002479"
                     /gene_synonym="iatA"
     CDS             complement(11737..13131)
                     /gene="ychO"
                     /locus_tag="ACHKAU_002479"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO5531528.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13316..13669
                     /gene="ychN"
                     /locus_tag="ACHKAU_002480"
     CDS             13316..13669
                     /gene="ychN"
                     /locus_tag="ACHKAU_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO5531529.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13713..14408)
                     /gene="chaC"
                     /locus_tag="ACHKAU_002481"
     CDS             complement(13713..14408)
                     /gene="chaC"
                     /locus_tag="ACHKAU_002481"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO5531530.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14566..14796)
                     /gene="chaB"
                     /locus_tag="ACHKAU_002482"
     CDS             complement(14566..14796)
                     /gene="chaB"
                     /locus_tag="ACHKAU_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO5531531.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15066..16166
                     /gene="chaA"
                     /locus_tag="ACHKAU_002483"
     CDS             15066..16166
                     /gene="chaA"
                     /locus_tag="ACHKAU_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO5531532.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16572..16679
                     /locus_tag="ACHKAU_002484"
     CDS             16572..16679
                     /locus_tag="ACHKAU_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5531533.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
     gene            17107..17214
                     /locus_tag="ACHKAU_002485"
     CDS             17107..17214
                     /locus_tag="ACHKAU_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5531534.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(17363..18217)
                     /gene="kdsA"
                     /locus_tag="ACHKAU_002486"
     CDS             complement(17363..18217)
                     /gene="kdsA"
                     /locus_tag="ACHKAU_002486"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO5531535.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(18253..19062)
                     /gene="sirB1"
                     /locus_tag="ACHKAU_002487"
     CDS             complement(18253..19062)
                     /gene="sirB1"
                     /locus_tag="ACHKAU_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO5531536.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(19066..19458)
                     /gene="sirB2"
                     /locus_tag="ACHKAU_002488"
     CDS             complement(19066..19458)
                     /gene="sirB2"
                     /locus_tag="ACHKAU_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO5531537.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(19455..20288)
                     /gene="prmC"
                     /locus_tag="ACHKAU_002489"
     CDS             complement(19455..20288)
                     /gene="prmC"
                     /locus_tag="ACHKAU_002489"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5531538.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(20288..21370)
                     /gene="prfA"
                     /locus_tag="ACHKAU_002490"
     CDS             complement(20288..21370)
                     /gene="prfA"
                     /locus_tag="ACHKAU_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO5531539.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(21412..22668)
                     /gene="hemA"
                     /locus_tag="ACHKAU_002491"
     CDS             complement(21412..22668)
                     /gene="hemA"
                     /locus_tag="ACHKAU_002491"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO5531540.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22882..23505
                     /gene="lolB"
                     /locus_tag="ACHKAU_002492"
     CDS             22882..23505
                     /gene="lolB"
                     /locus_tag="ACHKAU_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO5531541.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            23505..24356
                     /gene="ispE"
                     /locus_tag="ACHKAU_002493"
     CDS             23505..24356
                     /gene="ispE"
                     /locus_tag="ACHKAU_002493"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO5531542.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            24507..25454
                     /gene="prs"
                     /locus_tag="ACHKAU_002494"
     CDS             24507..25454
                     /gene="prs"
                     /locus_tag="ACHKAU_002494"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO5531543.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            25579..27258
                     /gene="dauA"
                     /locus_tag="ACHKAU_002495"
     CDS             25579..27258
                     /gene="dauA"
                     /locus_tag="ACHKAU_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO5531544.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(27313..27591)
                     /gene="ychH"
                     /locus_tag="ACHKAU_002496"
     CDS             complement(27313..27591)
                     /gene="ychH"
                     /locus_tag="ACHKAU_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO5531545.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27869..28453
                     /gene="pth"
                     /locus_tag="ACHKAU_002497"
     CDS             27869..28453
                     /gene="pth"
                     /locus_tag="ACHKAU_002497"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO5531546.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28570..29661
                     /gene="ychF"
                     /locus_tag="ACHKAU_002498"
     CDS             28570..29661
                     /gene="ychF"
                     /locus_tag="ACHKAU_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO5531547.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         7284 a         7947 c         7586 g         7342 t
ORIGIN      
        1 aaggttcgaa tccttccccc accaccatca cttttcaaaa gtccgtaaac tctcaaacga
       61 atccgcaatt aaatattctg cccaagcggg gaaggatgag aagcttcgac caaggttcga
      121 cttgagcgcc agcgagagag cgttgccgca ggcaacgacc cgaagggcga agcgcgcagc
      181 gttgagtaat ccttccccca ccaccatcac ttccttaaat aatcaaaacc tgttactccg
      241 agctaatttt gcccgatggc accacgttta ccgggcctac aatatcctcc cctgtaggtc
      301 ggataagcga agcgcatccg acaactcacc ccgcaccgtg cccataaaaa aaccccgccg
      361 aagcagggtc tatgtgaact aaaattcgct tagtgacgag cgcgcaccag ctggtactta
      421 cgtgtcagat actccaccgg tacgctccag atgtgtacca gacgcgagaa cgggaacagc
      481 aggaataacg tcatccccag caccaggtgc agacggaaga taaacgccac accatcgagg
      541 tgttgagaag cgccaccgtg gaaggtcacc accgactgcg cccagccaac cagtttcatc
      601 atctcgctac cgtccatatg ctgagcggag aacggaatgg tcaacaggcc cagcgcgcac
      661 tggataacga gcagcgacag gatcaggata tccgctccgg tagtggttgc acgcacgcgt
      721 gggctgaaca gacgacgctt cagcaacagc acgccgccaa tcagacacag cacgccgctg
      781 gcaccaccag caaacatcgc cattttctgt ttcacttcaa tcggcagcca ggcttcatac
      841 atccagtgcg gcgtcagcat accgaagaag tgaccaacaa aaatccccag aataccgata
      901 tggaacaggt tcgacgccag gttcatccct ttgcgatcca gcatctggct ggacgccgcg
      961 cgccaggtgt actgcccgta gtcataacgc agccagctac caatcaggaa gaccgccccg
     1021 gctatgtacg ggtagatatc aaagaagaac atattcagga attgcattag tgctgtcctc
     1081 cggtggtgat attcagatat tgcggcgcga cggcaccggc aaagcgacgc tgatgagcag
     1141 tgattgctga atcaccgcag cctttgtcag caaagaattt aacctgctct tcttcccaga
     1201 cagcatccag cgcctgcggc gtatcatcgc gcgcttcgtc ggcaattttt tccgccactt
     1261 tgtcgctgtc gatagcggta ttggccaatt tcagcagcag atcaaacatc acggcataac
     1321 ggctttcacg ctgttgcaga cgcgcgctca gcaatgccag aatcggcgcg atatctttca
     1381 aaccttccac ggcttcgctt tgcggcagct gcgacaggta ctccagatac agcggcagat
     1441 ggtccggtaa ttcgcggctg tttaactgca agccgtgctg ctcgtactgc gccagcaggt
     1501 ccaccatcgc ctggccgcgg tcacgggatt caccgtgaac atgttcaaat agcaacagtg
     1561 acgtggcgcg gccacggtcg aacagttcgc tgtactgggc ctgggcatcg agcggatcca
     1621 tcgccgttaa atcgcgcagg aaaatgccca gcgcatgggc atcttctttc gacagatttt
     1681 tcgacgcagc aatcgcctca aacatctctt gttgatgctg ccataaggca gcatccggat
     1741 attcaaggag acgcgataca atcacgagtt cgatcatgga tgcggctccg ttttgctggt
     1801 cacatcgatg gcatcgatac gacggctgtt gaacagattg aatttggtat ctgaaccgtg
     1861 gcagccatca ccaaaggtaa agccgcagcc atttttctcc gggaaggctt cccgtgccag
     1921 ttcacgatga ctactcggca ccacaaagcg atcttcgtag ttagcaatcg ccagataacg
     1981 gtacatctcc tgtgcctggg cttcggtcag accgacctct tccagcgcac gggtatcaac
     2041 tttaccgtca acggtttcag cacgtttgta atgacgcatc gccaacatac gtttcagtgc
     2101 gcgcagtacc ggtttggtat caccggcggt cagcagattc gccagatact gtaccggaat
     2161 acgcaagctt tcgacgtctg gcagaatgcc gttgctaccc agctcacccg cgtctgctgc
     2221 agactgaatc ggagacagag gcggcacgta ccagaccatc ggcagtgtgc gatattctgg
     2281 atgcagcggc agcgccagtt tccattccat tgccatttta taaaccggcg actgctgtgc
     2341 ggcttcaata acgctcagcg gaataccgtc tttaatcgcc tgctcgatga ctttcggatc
     2401 gttcggatcg aggaacacct ccagctgacg ctggtaaaga tctttctcgt tctcggtgct
     2461 ggctgcacgt tcaatcgcgt cggcatcgta cagcagcacg ccaagataac ggatacgacc
     2521 gacacaggtt tctgagcaca cggtcggctg acccgcttca atacgcggat agcagaagat
     2581 gcacttctca gacttaccgc tcttccagtt gaagtagatt tttttgtacg ggcatccggt
     2641 gatgcacata cgccagccac ggcatttatc ctggtcgatc aggacgatgc catcttcttc
     2701 acgcttgtaa atcgcaccgc tcgggcaggt cgccacacat gccgggttca ggcagtgttc
     2761 gcacaggcgc ggcaaataca tcataaaggt gttttcgaac tggctataca tcaccttctg
     2821 gatgttgtcg aagttcttgt ctttcgccag tttgtcaaac tcaccaccca ggtcatcttc
     2881 ccagttcggc cctttttcga ttttcgccat ccgttccccg gtaatcagcg aacgcggacg
     2941 ggcaatcggc tgcgatttgc tgccttccgg cgcggtatgc aggttctgat agtcaaaatc
     3001 gaacggctcg taataatcgt cgatccccgg cagatgcggg ttagcgaaga ttttacccag
     3061 caacatggca cggttaccca tgcgcggctg cagtttgccg ttgattttac ggatccagcc
     3121 gcctttgtat ttttcctggt tttcccagtc agtcgggaag ccctgacccg gcttggtttc
     3181 cacgttgttg aaccacgcgt attccacgcc ttcacggctg gtccagacgt ttttacaggt
     3241 aactgaacag gtgtggcagc cgatgcactt atcgagattc agcaccatgc cgacttgtga
     3301 acgaattttc attttacgct ctcctgtacc tggtcattgc cttcgccatc taaccagtca
     3361 atgttcttca tcttacgcac tacaacaaac tcatcgcggt tagaacctac ggtgccgtag
     3421 tagttaaagc cgtatgccag atgggcatag ccgccgatca tatgcgtcgg tttcggcgtg
     3481 atacgggtga ccgagttatg gataccacca cgctgttggg taatttccga accaggcagg
     3541 ttaacgatac gttcctgcgc gtggtacatc atggtcatcc ctgccggaac acgctggctg
     3601 acaaccgcac gggcagtcag agcaccgttg ctgttgaaga cttcaatcca gtcgttatcg
     3661 gcgataccca gatctttggc atcggcttca ctcaaccaga ccaccggacc accgcgacct
     3721 aaagtcagca tcagcaggtt gtcgctgtag gtggagtgga taccccactt ctggtgcggc
     3781 gtcaggaagt tgagcgcttt ttccgggttg ccattggatt tctggcccat cacttctttc
     3841 accgaacggg tgtcgatcgg cggacgataa accagcaggc tttcaccgaa atcacgcatc
     3901 cattggtgat cctgatacag ctgctgacgg ccagagagcg tacgccacgg gatcagctcg
     3961 tgaacgttgg tgtaaccggc gttgtaagaa acgtgttcat cttcaaggcc agaccaggtc
     4021 gggctggaga tgattttgcg cggctgtgcc tgaatatcgc ggaagcgaat cttctcgtct
     4081 tctttattca gcgccagatg cgtatggtca cgaccggtaa actcgctcag ggcagcccag
     4141 gctttcacgg ctacctgacc gttggtttcc ggtgccagcg tcaggatcat ctctgccgca
     4201 tcaattgcgg tgttcagcat cggctggcct ttcgccggac cttccgcttt ggtgtagttg
     4261 agcttacgca gcagatccat ctcgctctgg gtgttccagg caatcccttt accgccatta
     4321 ccgattttct ccatcagcgg gccgatagag gtaaagcgtt cgtaagtcgc cggataatcg
     4381 cgctctacga ccataatgtg tggcgcggtt ttaccaggga tcaggtcgca ctcgcctttt
     4441 ttccagtctt tcacatccag cggctgcgcc agttcagcgg cagagtcgtg ctggataggc
     4501 agcgtgacga tgtcggtttc tttacccaga tgaccgacgc acacttcgga gaatttcttc
     4561 gcgatggctt tgtaaatttc ccagtcgctt ttcgcttccc aggccggatc gaccgccgca
     4621 gacagcgggt gaataaacgg atgcatatcc gaagtattca tgtcgtcttt ttcgtaccag
     4681 gtcgccgtcg gcaaaatgat gtcggaatag agacaggtgc tcgacagacg gaagtccagc
     4741 gtaaccacca gatccagctt gccttccaga ccattgtcct gccagtccac ttcttccggc
     4801 ttcacgccgc cctgttgccc cagatcttta ccctggatac cgtgctccgt ccccagcagg
     4861 tacttgagca taaactcatg acctttaccg gaagaaccga gcaggttaga acgccagatg
     4921 aacaggttgc gcgggtggtt tttaccgttt tctggttgtt ctgccgcaaa acggatggaa
     4981 ccctctttca gggatttcac cgtatagtcc accggattca tcccggcttt ttccgcttcg
     5041 ccagcgatag tcagcgggtt agtgcctaac tgcggtgcag acggcagcca gcccatgcgt
     5101 tccgcacgga cgttaaagtc gatcaagtgt ccggtatagc gggatttgtc cgccatcggt
     5161 gacagcaact cttccgccgt gacggtttca taacgccact ggctggagtg gttatagaaa
     5221 taagaagtgc tgttcatgtg acgcgccgga cgctgccagt caagggcaaa cgccagcggc
     5281 tgccagccgg tttgcggacg cagtttttcc tgacctacat agtgcgccca gccgcccccg
     5341 ctctgaccga cacagccgca gaaaatcagc atgttgatca gaccacgata gttcatatcg
     5401 aggtgatacc agtggttcag ccccgcaccg acgataatca tcgaacgacc gtgcgtttta
     5461 tcagcgttat cggcaaattc acgggcgatg cgaataatct ggctgcgaga aacgccggta
     5521 atctgctcgg cccaggccgg ggtataagct ttcacatcgt catagctggt tgcacagtta
     5581 acgtcgttca ggccacgttc cagaccgtag tttgccagcg tcagatcata aacggtggtc
     5641 accagggcgg tgctgccatc agccagttgc aggcgtttca ccggcagttt gtgcagcagc
     5701 acgttttcca gttccacttt gttgaagtgt tccgtgccgt cgccaccaaa gtacgggaag
     5761 cctacctcag cgatctcatc ctggctaccc agcaggctca gttgcagctc ggtttcttcg
     5821 ccagttttgc cgtcgcgctg ctcaagattc catttgccct tctcgcccca gcggaagcca
     5881 atagaaccgt tcggcgcaac catttcgcca ttggtattaa aggcgacagt tttccattcc
     5941 ggattgtttt cctggcccag cgcatcaacc agatcagcag cgcgcagcat acgacctgca
     6001 gcgtagtaac cgtcgcgttc ttccagcatc accagcatcg gcatgtcggt gtagcgacgc
     6061 acatagtcgg tgaaatactg gcttgggttg tcgaggtgga attcacgcag cattacgtgg
     6121 cccatcgcca gcgccattgc cgcatcggtg ccctgtttcg gtgccagcca cagatcgcac
     6181 agtttggcga tttcagcgta gtctggtgtg acggcaacag ttttggtccc tttgtaacgc
     6241 acttcagtaa agaagtgagc atccggggta cgcgtctgcg gtacgtttga cccccaggcg
     6301 atgatgtagc tggagttgta ccagtcagca gattccggta cgtcagtttg ctcgccccag
     6361 gtttgcggag acgcaggagg caagtcgcag taccagtcgt agaagcttaa gcaagtaccg
     6421 ccaatcagcg agagatagcg tgcacccgat gcgtaagaaa ccatcgacat tgccggaatt
     6481 ggcgagaaac cagcaacacg gtccgggccg tagtttttga tggtgtaaac gttagatgcg
     6541 gcgatcagtt cgttcacctc ctgccaggaa gaacgaacaa atccaccgcg tccacgcgcc
     6601 tgcttaaagc ttttcgcttt atcggcgtct tcaatgatag aagcccatgc ctcaaccgga
     6661 tcgctatgca gcgccttcgc ttcacgccac attttcatca ggcgtttgcg catcatcggg
     6721 tatttcaggc ggttggcact gtaaagatac caggagtagc tggcaccgcg cgggcagccg
     6781 cgaggttcat ggtttggcag atccggacgg gtacgcggat agtcagtctg ctgggtttcc
     6841 caggtgacca gaccgttttt gacgtagatt ttccagctgc aggagccggt gcagtttacc
     6901 ccgtgggtag agcggacgat tttgtcatgc tgccaacgct ggcgatatcc atcctcccag
     6961 tcacggttgg tattgagaag ctggccatgc ccatcggcaa aggtttcacc cttctgtttg
     7021 aagtagcgaa accggtccag gaatttactc atcggttttc tcctgtggga gcctgtcggc
     7081 cctctgataa atcgacattg ctaatgttta agggcaaggt aacgctctga aacagcgtgg
     7141 gaattgataa cgatcaagtg tgatggggat gaaaaataaa gtaaattcct aagatacccc
     7201 tttaggataa ttaacagtta aagcataaca tgctgtaaat taagacgttt tatacctgta
     7261 gttatttaaa gcatgaagat ttttatgcag atgggtatta aggagtattc cccatgggta
     7321 acatattaat ggctcttaat gaaatccggc gaataagcca tataacatta gtatgttgta
     7381 actaaataac gcataaaaaa agcgcgacat catgccgcgc aaaggataat caaatatttt
     7441 actctatata attcggttta caggaaggcg gcaagtgaat gaatcccagg agcttactaa
     7501 agtaagtgac tggggtgaat gaacgcagcc gcagcacatg taacccgaag tatgacgagt
     7561 aacggtttat tttttagaat gccgaccata taccgcccag gtaatcacta cgcaggcgat
     7621 atagaaaatc aaaaatacct tcattgcgcc gactggcgaa cccgttaatg ccagcgagct
     7681 accaaacgct ttcgggataa agaagccacc aatagcgcca atcgcagaga tgaaacccag
     7741 cgccgctgcc gtgtcggttg ccgcttcacg catcgcacgt tcgtcagaac ccccttctgc
     7801 tttcacgcga tccattgtca gtttacggaa gatcactgaa atcatctgga aagtggaacc
     7861 actacccagc ccagctgtca ggaacagcgc caggaagacc gcgaagaacg ccatgaagct
     7921 tccgccctgc ccgtcagtcg gtaaggtcag gaacagcagg ccgctgaaaa tcgccatcag
     7981 aataaagttc accagcgtga cacgagttcc gcccagacgg tcagataatg caccacctgc
     8041 agaacgcgcc agcgcaccaa taaacggccc gaagaaagcg tattgcagaa tctgaacatc
     8101 cgggaactgc gtttttgaca gcattgcaaa gcccgcggag aagccgatga aggagccgaa
     8161 ggttgccaga tacagcaggc tcataatcca cagatgaccc cgtttgagta ccggcaactg
     8221 ctccttgatg gaggctttcg aggtagcaag atcgttcatg ccaaaccacg ccgcaatagt
     8281 gaagatggca aggaacggca cccatatcca ggacgcattc gccagataca gctcagtccc
     8341 atccggctgt ttgacgccct ggctaccaaa tactgcgaaa atcgacagtg ataccaccag
     8401 cggagcaacc aactgcatga cgctgacgcc catgttgccc agaccaccat tcagacccag
     8461 cgcgccaccc tgcttctgtt tcggaaagaa gaagctgatg tttgccatac tggaggcgaa
     8521 gttcgcgcca gcaaagccgc atagcagaga gatgatgatg aagacgctat aaggcgtgga
     8581 ggtatcctgc acggcaaaac ccagccagac gcaaggaata atcagaatac cggtgctgaa
     8641 cgccgtccag cgacgaccac cgaagatagg aaccataaag gagtatggaa cacgtaataa
     8701 cgcgccagaa accgaaggca gcgcagtcaa cataaatagc tgatcggtcg taaaattaaa
     8761 gccgactttc ggtaggttca ccgcaacagc gctgaacaac atccatacgc aaaacgccag
     8821 cagcagacag ggaacggaaa tccacaggtt gcggctggca atacgttgac cgcgttgttg
     8881 ccagaacgca ggatcttccg gtcgccaatc tgtaatgaca gctccagtag ccctttcggg
     8941 ggcggatgag tgactcatag acacctctga tactcgtttc gatgtctgcc accttagtgt
     9001 ctgtagctaa aggcaatttg atgtaaatca aacgataaga taactttatc tatcattgat
     9061 atttatcatt acccatagtg agtacagtga tttcataaaa attatgagat ttttcacggt
     9121 gctgtagaat ccctaccctt aacgatgtaa agcgactaac cacacggcaa ataaggagta
     9181 actctttcca ggtatgggta tacttcagcc aatagccgag aatactgcca ttccagaatg
     9241 tatcgtcaca ttcattaagg ttattgctca tttaaagcct gaaggaagag gtttacatgc
     9301 ttaaacgttg tctctctccg ctcaccctgg ttaatcaggt tgcgcttatt gtgttgcttt
     9361 ctactgctat tggactggca gggatggcgg tttctggctg gctggtgcaa ggcgttcagg
     9421 gcagcgccca tgcgatcaac aaagcgggat cgctgcgcat gcaaagttac cgtctgttgg
     9481 cagcagtgcc attaagcgag aaagacaagc ccttaattaa agagatggaa caaacggcat
     9541 ttagcgccga gttgactcga gcagcagaac gagacggaca actggcgcaa ttacagggtt
     9601 tacaagatta ctggcgtaat gaactgatcc ctgcgttgat gcgtgcacaa aaccgagaaa
     9661 cggtgtcagc ggatgtcagc cagtttgttg ccgggcttga tcaactggta tctggttttg
     9721 accgcaccac ggaaatgcgc atcgagacag tggtactggt ccatcgggta atggcggtat
     9781 ttatggcact tttactggtg ttcactatta tctggttgcg ggcgcgactg ctacaaccgt
     9841 ggcggcaact gctggcaatg gcgagtgccg tcagtcatcg cgattttacc caacgcgcaa
     9901 acatcagcgg gcgcaacgaa atggcgatgc ttggaactgc gttgaacaat atgtctgcag
     9961 aactggccga aagttatgcc gtacttgagc agcgggttca ggagaaaacc gccgggctgg
    10021 agcataaaaa tcagatcctc tcttttttat ggcaggctaa ccgccgtttg cattcccaag
    10081 ccccgctgtg tgaacgcctg tcacctgtac tcaacggctt acagaattta accctgctac
    10141 gtgatatcga actgcgggtt tatgatactg atgatgaaga gaatcatcag gagtttacct
    10201 gccaaccaga tatgacttgt gatgataaag gctgccagct ctgcccgcgc ggcgtattac
    10261 ccgttggtga tcgcggcaca accctgaagt ggcggctggc tgactctcat acgcagtacg
    10321 gtattttgct ggcgaccctg ccgcaggggc gtcatcttag ccatgatcaa caacaactgg
    10381 tggataccct ggttgaacaa ctcaccgcca cgctggcgct ggatcgccat caggaacgtc
    10441 agcaacagtt gatcgtgatg gaagagcgtg ccaccattgc gcgcgaactg catgattcta
    10501 ttgcccaatc tctctcttgc atgaagatgc aggtgagttg tttacagatg cagggcgatg
    10561 cgctgccaga aagcagccgc gaactgttaa gtcagatccg taacgaactg aatgcatcct
    10621 gggcgcagtt gcgtgaattg ctcaccacat tccgcttgca gctcaccgag cctggattac
    10681 gtccggcgct ggaggcgagt tgcgaagagt acagcgccaa atttggcttc ccggtgaagc
    10741 tggattatca attgccgcct cgcctggtgc cttcgcatca ggcaatccac ttgttgcaaa
    10801 ttgcccgtga ggcattaagt aacgccctca aacactcgca agcgagtgaa gtcgtggtga
    10861 cggtggcgca aaacgataat caggtcaaac tgaccgtcca ggataacggc tgcggcgtgc
    10921 ctgaaaatgc catccgcagc aatcactacg gcatgataat aatgcgcgac cgtgcgcaaa
    10981 gtttacgagg cgattgccgc gtccgccgtc gtgaatcagg tggcaccgaa gtggtggtca
    11041 cctttattcc cgaaaaaact ttcacagacg tccaaggaga tacccatgag taatcaggaa
    11101 ccggctacta tcctgctgat tgacgatcac ccgatgctgc gaactggcgt aaaacagctt
    11161 atcagtatgg caccagatat caccgtggtt ggcgaagcga gtaatggcga acagggtatt
    11221 gaactggcgg agtctcttga tcccgatctg atcctgttag atctcaatat gcccggcatg
    11281 aacggtctgg aaacgctgga taaactgcgc gaaaagtccc tctcagggcg cattgtggta
    11341 ttcagcgtct ctaaccatga agaagatgtg gtcaccgcac tgaaacgcgg cgcggatggt
    11401 tatctgttaa aagatatgga accggaagat ctgctgaaag cattgcatca ggcagctgct
    11461 ggcgaaatgg tattaagcga agcattaacg cctgttctgg ccgccagctt gcgcgctaac
    11521 cgtgccacta ctgagcgcga tgttaaccag ttaaccccac gcgagcgcga tattctcaag
    11581 ctgattgccc agggtttgcc gaacaagatg attgcccgcc gcctggatat caccgaaagc
    11641 acagtaaaag tgcacgtcaa gcacatgctg aagaaaatga agctcaagtc tcgcgtggaa
    11701 gcagcggtat gggtgcatca ggagcgcatt ttctgattac ggttcccagc gcagttcgtc
    11761 gtttgacaat gcatcgaacg gttcgacaag cgttagcgtg atctcattgg aggagacacg
    11821 ctgcccctgg ttatcttcca ccaccaccga caatcgccag tgattgcttg ccccttcccc
    11881 gttctgccag tcaggcatga tcagcgtcca gccctccgcg ctgttggctt gtgcgcccgg
    11941 cgtcaaactt aatatctgcg tatcgccctg ccaaatcagt tgccgaatac cgtaacgact
    12001 gcggatttgt aatttcagcg gcactgtttc gccaggtttt agatcccacg gcggtgtcgc
    12061 cagaaacacc gttaacgttt ttcgctgtcg gtactcaaga gtcggtagat tatttcgctg
    12121 cggattgtca tagcgactac cacgtaacga ctgactttcg gcaacctcac ccgcagaaag
    12181 ttgttttttg agcggtacac caaagcggta attaagattc agcccgaggt tattttgatt
    12241 ctcgccactt tcaccctgtt tatgctgggc cgtcacagtg actaatggca caggggtgta
    12301 atttaatccc agactcaacg cgacgggatt gtgataaccc gtaccagagt taaacaaatc
    12361 aacacgatca ccaaaatact gttctacgct gacactggta ttgaggtgtt gatagaacgg
    12421 catgcgcatc cgggctgtca ggtcataccc gcgcgccatc cgttgttcct gcgtggctgt
    12481 ctgttcatgc catgcagcaa acggctgata aaagtttgcc gatagtcgca aatattcgcc
    12541 ccacgcttcg gcaccaaagc ccgctcgctg aagattttcg tccagcaagt tgtcataaaa
    12601 agtgttataa cccaccagcc agttgccgcg cgcccagcgt tgcccaacgc ccacattgct
    12661 caccaaccca tcatcctgct gagtaagacc aagctggctc caggtgagat aacgatcatt
    12721 atcttgtaac ggcacaaacc agcttccacg actgccggtg aaatgtcctt cgttatccac
    12781 tttgacgtca acactggcat ttccccacgg tgatagccag gactctacgt gctgattaac
    12841 ctgttgacta agcgcgtcgc ggacttttcc caatgcgaaa gcttttgcct gctcgccagt
    12901 atccagcccg ttatcattca tactggtttc gccgaaatct ttcacaattt cagcaaagtg
    12961 tttttcccca tcatgatttt cgggagccat gcctaaatcc ggcagaccat cattgttatt
    13021 atcaaaggga tttgccgctt tttgctcgaa ggtagattgt gcgttagctg taccgcctgc
    13081 tacaagcaag agtaaataaa acgggataat gcgaggaacg aaacggctca aaacgtcggt
    13141 aacttccggt aataatgaaa aaccatagcc ttaaagaata acgattactg gcaacttttg
    13201 tcgtattttc tcgcgctttc aggaaaatcc ttaaaaatgt gaccgattgg tcagacgctg
    13261 cagaagcagt aagatagaag atgaatgtct aattccctga atacaggagt aaataatgca
    13321 aaaaatcgtg atcgttgcca atggcgcacc ttacgggagc gaatccttgt ttaacagctt
    13381 gcggctagcc attgcgttac gagagcagga gagcaatctg gatctgcgtc tgttcctgat
    13441 gtctgatgcg gtcacagccg ggttgcgcgg gcaaaaacca ggggaaggct acaacattca
    13501 gcaaatgctg gagatcctta ccgctcagaa tgtaccggtg aaattgtgca aaacctgtac
    13561 cgacgggcgc gggattagta cacttcctct gattgatggg gtggaaatcg gtactcttgt
    13621 ggaactggcg caatggacgc tgtcagccga taaagtgctc acattttaat gtttgagggg
    13681 gcgtctgacg ccccactatg agggaagttt actcaggcga atcccggccg taacacacca
    13741 tccggataat tctccgccag cagttttttt accgatacca gcaaatcatt cagcccatcg
    13801 tcctgcattc ccagtttgat aagctcctgt tccagtgaaa acaggtattg tgcgttggtt
    13861 cccagcggac cgctcgccgc cgcaatcaac ggcgcgatga cctgagcgcg agtgtcagat
    13921 tcatattctg gatgtcgcgg gtccataata aacacaatgg cgtttactgt gcatccatca
    13981 tcaagatcaa gctgacacca ggttggcaga taacagccgg taatcatctc gcgcttccac
    14041 aacagggtta gttcctgctc cagcgtctct tctggcagtc gataggcgac gcctgtagtg
    14101 cgtccgccct ctttcagtgc aagcatccgt cccggctggt gcgcagtccc gcgcccggcg
    14161 gtcaggcgca ggcaaaacgc gcgatgccat ccaaccagtg taccggtgca cgactcggta
    14221 aactccagtg ccggattcca catcaacgat ccataaccga agatccacac cggtccctca
    14281 tcaggtcgac aagccagcgt cgccgccagc gaagccgccc gttgctctgc tgaccataag
    14341 agtgattctt caatggcacc aaatgccgtt ttacaatcgg cattcatcaa gaaatcacgc
    14401 gttatcacct tacacctccg ccactgcata cttccctgcg acaacttttt ggtgtcttcc
    14461 ggacattttt actgctcatt ttgaaaccac taacgggaca atatgcaatc cacggaccac
    14521 ttgcaaggca acaccgcacg aatagcttta actaagctac cggttttacg attttttatg
    14581 ccatttatca tcatcccctt tggcatattc atgcttcaca gcagcccagg ccactttatg
    14641 cgcggtttct tcgcgactgg cgtcatcacg ccgatcgtct ttatctttat attgatccca
    14701 tgcgctgttg aacgcttctt tatagatatc ctgggcatga gacggtagaa cgtgctttac
    14761 gctttccggc agatcgcttt tcgttttata cggcatcgtt gactcctttt acaaagttga
    14821 acattaagtg tggtcaacaa tatctactct tgccaaaaca taagaatttt catgtcagac
    14881 tgttcttaag aaacgctaaa acaaacattg tctaaacgta atacttattt ttatgtccca
    14941 atgagtaaaa aatcgacagt ggggtaaaaa ttcgtaaaat gttaccgata tccagacact
    15001 ttctgttatt tttcctacct attttttaca ttttcgataa attcatctgc aaagggataa
    15061 ccataatgtc aaatgctcaa gaggcggtaa aaacccgcca caaggagact tcgcttattt
    15121 tcccggttct ggcgctggta gtgctgttcc tgtggggaag cagccagaca ctaccagtgg
    15181 tcattgccat caatcttctt gcgcttattg gtattttaag tagcgccttt agtgttgtcc
    15241 gtcatgcgga cgtattagcc catcgcctgg gagaacctta cggttcgctt attcttagcc
    15301 tttcagtggt tattcttgaa gtcagtttga tttcagcttt aatggcaacc ggcgacgccg
    15361 cgccaacgct aatgcgtgat acgctctatt caatcattat gattgttacc ggtgggctgg
    15421 ttggcttttc attattgttg ggcggtcgta agtttgccac ccaatatatg aatctgtttg
    15481 gtatcaagca gtatttaatt gcgctgttcc ccctggcgat aatcgtactg gtatttccaa
    15541 tggctctgcc tgcggcgaat ttttcaaccg gtcaggcgtt actggtagca ttaatttctg
    15601 cggcaatgta tggcgtattt ttgctgatcc agaccaaaac gcatcaaagt ttgtttgtct
    15661 acgagcacga agatgacagt gatgacgatg acccgcatca cggtaaaccg tctgcccata
    15721 gcagcgtgtg gcatgctatc tggttgatta tccatctgat tgccgttatt gcagtcacca
    15781 aaatgaatgc cagcccgctg gagacattgc tcgacagcat gaatgcccct gtcgccttta
    15841 ctggcttcct ggtggctctg ttgattctgt cgccggaagg tttaggtgcg ttaaaagcag
    15901 tgttgaacaa ccaggtccag cgcgcgatga atctgttctt tggttcagtg ttagcaacca
    15961 tttcgcttac cgtacctgtc gtcacgctaa ttgcctttat gacgggtaac gaattacagt
    16021 ttgcacttgg tgcgccagaa atggtggtga tggtggcctc tttagtgctg tgccatatct
    16081 ccttctccac cggacgtact aacgtgctca atggcgcagc gcatctggca ctgtttgccg
    16141 cctatttgat gacgatattt gcctgacagt tatatctcct ccgatgacgg aaatcggggg
    16201 agataagata aattcctgct tattgtattt cttcatacta cctggacagc tcgttaatca
    16261 gaattgcgag cctgtaccac aacctgcacg aaaccactgc aacagctgtt gaaaagccgg
    16321 aatgtactct ttattgcaaa catttcaggg atgaagtgaa gatgaattgc gacgtagtat
    16381 ccacaccaag ccggcgttat ccggtgaggc gcaatgttgc gggggcttta tccctggtgg
    16441 cattggttgc tggaaagaga aaacccccgc acgttgagag gtaaaacctg acaacagaac
    16501 gggggctaat cttgaaccat caacaattca agaatagccg caaaatgttg tcattacaac
    16561 aaggcggcta tatgacgctc gcgcagtttg ccatgatttt ctggcacgac ctggcagcat
    16621 cgatcctggc gggaattatt accgcagcga ttgtcggctg gtggcgtaac cggaagtaag
    16681 gcgaaaacgg gcgtcaggct gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt
    16741 aacagcctgc ggcctttttt gtctttatct cttccgccag taaacattgg caacccgagc
    16801 tggaagcatc atccagagcc ctcatgaaac gactgtaaca gctgttgaaa aaccggaatg
    16861 cactcttcat tgcaaacatt tcagggatga agtgaagatg aattgagacg tagtatccac
    16921 atcaagccgg cgttatccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt
    16981 ggttgctgga aagagaaaac ccccgcacgt tgagaggttt aacctgacaa cagaacgggg
    17041 gctaatcttg aaccagaaca attcaagaat agccgcaaaa tgttgtcatt acaacaaggc
    17101 ggctatatga cgctcgcgca gtttgccatg attttctggc acgacctggc agcaccgatc
    17161 ctggcgggaa ttattaccgc agcgattgtc ggctggtggc gtaaccggaa gtaaggtgaa
    17221 aacgggcgtc aggctgccgt gatggcaata tgcgcccgaa accggccgca ggggtaacag
    17281 cctgcggcct tttttgtatc tggcacccat gaaaaaagtc ttaacgcaga acgctaagac
    17341 tttatttttc aagcaaaaaa gattacttgc tggtatccag ttcttcgaaa cctttcacca
    17401 gatcatcaat cgctttcatc tgcttgagga acggttccag tttagccagc ggcagcgcgg
    17461 atggaccatc acatttcgca tgttccggat ccggatgcgc ttcaataaac agccccgcca
    17521 gacctaccgc cataccggct cgtgccagct cagccacctg agcacgacga ccaccggaag
    17581 ctgcgccaaa cggatcgcgg cattgcagtg cgtgggtcac gtcgaaaatc accggcgagt
    17641 taccagacac tttcttcata atgctgaagc ccagcatatc gacaaccagg ttgtcatagc
    17701 cgaagttagc accacgatcg caaagaatca ctttttcgtt gccgccttct ttgaatttat
    17761 caacgatatt acccatctgt cccgggctga caaactgtgg tttcttgacg ttaattaccg
    17821 caccggtttt cgccatggct tcaaccaggt cagtctggcg agcaagaaac gccggcaact
    17881 gaatcacatc cacgacatca gcaacgggct gtgcctgact tggttcgtga acgtcggtga
    17941 taattttcac gccaaaagtc tgcttcaact cctggaagat tttcatccct tcttccaggc
    18001 ccggtccacg ataagagtgg atggaggagc ggttggcttt gtcaaaagag gctttgaaca
    18061 cgtaagggat acccagtttc tgggtcacag ttacgtagtg ctcgcaaatg cgcatcgcca
    18121 gatcgcgaga ttccagcacg ttcataccgc caaacagtac gaacggcagg tcatttgcta
    18181 cgttgatgtc gccaatgcta accacttttt gtttcatagg atcgccttaa tcttgagtaa
    18241 aatgtcgatt aattaatgca gcacaatatg tttatgcgcg atgttattta tttgcgcacg
    18301 gatcatttcg ctgatcgggt cttccggaca ctgttcaacg aaataactta aatcgttcaa
    18361 cgcaacgtgt tcgcaatcca gttgcacata aatcaacccg cgatcgcgaa tttcataggg
    18421 atcttcaggg ttgaattgta ataaagcttc gctggtgcgt aacgccagct ccatctgatt
    18481 ttcttccatc aacgaggctt tgagtgtatc cagcaatttg cggattacct caatgttatc
    18541 agcttcatca aggtcttcat aaaacagttc cgccgacggg ctgatatttc cctttaacca
    18601 tacgtccagc atatgttcgc ttaacgattc accgttaaaa ggattaatca gccaaatttc
    18661 gccatccgga cattcaatgc gcaatatcag ctgcgtaggg aaaatcaccg gcagcagcgg
    18721 caaatcgaga cgattcgcga cccataataa aaccgcacct aatgataccg cactgccctg
    18781 tcgattcttt aacacctggt ccagccataa tgcatcggaa agacgataaa cgccgcgtga
    18841 ggctttaaat ccccagtcgc cgtaaaacag cgcgatcagt ttttccagct gctcttctaa
    18901 aggcagaagc tggctgattt cttccttcgc cagactaacg agacgctcca gttcgtcgta
    18961 aacatcttgc gagggaaaat cgcggcggat tgcttcgcaa gccaggatca tgccttcgca
    19021 caatggcgct ttattaaatt cgaaatcagc taacgatctc atgacttacc ccagtaacgg
    19081 tacttttgtg gtggcgagtt taatgatgat gtacagcacc accaacgcca gcgggaaggc
    19141 aataatgcgc gcctgctgac tgtgcatacg acgataatcg agtgcaataa aacccaaaac
    19201 gatataaatg ataactccaa acagcttttc agtcagccac tgtgcctgcc cggaaaatgg
    19261 caggatgtgc gctttagcca tcaacgcaat gccgcttaac aataacaccg tatcgacaac
    19321 cggcggcaca atgcgcgtcc agcgagcaaa tgcctgtgga tgcttctgat aacgtagcca
    19381 gaaacgtaag gttaatagcc caacagaaag cgcgatacta ataagatgaa cactaagcag
    19441 tgtagaaaaa cttgtcattg ataatagcgg ccgagcgtta cgcgctcgtt atcaccatag
    19501 tcacggcagg tttcgacgtc atgatacccc gcgaggataa atgcttgtcg caccgcttcg
    19561 ccctgctgcc agccatgttc cagaagcaga aagccgccgg ataccagcgc gttacgcgac
    19621 tgttcgatga tatgcacgat gtctgccatt ccactgtctg ccgcaaccag cgcagtgagc
    19681 ggctcaaagc ggacatcgcc ttgttgaaga tgtgggtcct gctcgtcaat atacggcgga
    19741 ttgctgacaa tcatcgcaaa ctgctgcccg gctagcgcgc taaaccagtc gctttgcaga
    19801 atgtggatat ttttgatcgc cagatgctgg gcattacgtt gtgccaggga gacagcatca
    19861 ggcatacgat ctacagcgat aatttcgcag tccgggcgct cgctagccag cgcaagcgca
    19921 atcgccccgg tacccgtccc gagatcaaga atacggcaag gttgttcagg caaccgcgcc
    19981 agtgcctgct ccaccagaca ctccgtatcc gggcgcggaa ttaaggtcgc tggcgaaaca
    20041 aataacggca acgaccagaa ttctcgcacc ccggttaaat gagcaatggg ttcaccatcg
    20101 cgacgacgtg tcagtagcgc atcaagttgc tgacattgtt cgtcagtcag ctgcgtttca
    20161 ccaaaggcga gaataaaagt acgccctctg ccggtaacat gctccagcag gatttcagca
    20221 tcacgccgcg ggctttcgct cgcctgaagt tggcttattg cttcacgtaa ccagtgttga
    20281 tattccatta ttcctgctcg gacaacgccg ccagttggtc ggcctgatgt tcctggataa
    20341 tcggttcaat cagcatatcc agcttacctt ccatcacttc atccaggcgg tagagcgtca
    20401 ggttgatgcg gtgatcggta acgcgcccct gcgggaaatt gtaagtacgg ttacggtcgc
    20461 tgcgatcgcc actccccagc aggttacgac gggtagacgc ttcggcctgt tggcgttttg
    20521 ccatttcagc agcgtggatg cgagcaccga gcactgaaag cgccttcgct ttgtttttat
    20581 gttgtgaacg ttcatcctga cattcaacaa caatcccggt cggcaagtga gtaatacgaa
    20641 ttgccgaatc ggtggtgtta acgtgctgac cacccgcccc tgacgagcgg aaagtatcaa
    20701 tgcgtaaatc tgctgggttg atgtccggca gttctgcgtc aggcagttct ggcattaccg
    20761 caacggtaca agcagaagta tgaatacgac cctgcgattc cgtagcagga acacgctgca
    20821 cgcgatgacc gccagattca aatttcagac gaccatacac accatcaccg ctaattttgg
    20881 cgatgatctc tttataacca ccatgttcac cctcgctggc gctcatgatt tctacccgcc
    20941 agcggcgggc ttcggcgtaa cggctgtaca tacggaacag atcgcctgcg aacagcgccg
    21001 cttcgtcgcc gccggttccg gctcggactt cgaggaaggc gttacgttcg tcatcaggat
    21061 cttttggcag taacagaacc tgtaattgct gttccagttg ctcgcttttt tctttagctt
    21121 cgcgcagttc atcctgcgcc atctcacgca tttcaggatc atcgagcatc atctgtgcgg
    21181 tttcgatatc ttcctgaacc tgttgccagt cggtaaaaca gcgcgaaaca tcacttaact
    21241 gcgcatattc gcgtgataat gcgcgaaaac gttcctggtc ggcgatagtt tgcgcgtcac
    21301 ccagcaacgc ctgaacttct tcatggcgtt catgcagggc ttccagtttg gcaacgatag
    21361 aaggcttcat aggcgtaaat gcaccctgta aaaaaagaaa atgatgtact gctactccag
    21421 cccgaggctg tcgcgcagaa tattcaggcg ttcgttatcc ccgtcacggg cggcctgttg
    21481 aagtgatttc gttggcgcat ggatcaagcg gttagtcagt ttccatgcca gatcctgcat
    21541 aatggcttgc gcgtcgccgc cctgctcaag ggccgctaac gctttggcgg ttaactcatc
    21601 gcgaacatgc tctgcctggc tgcgatactc gcgaatggtt tcgctggcgc tttgtgctcg
    21661 cagccacgcc ataaattcgc tggtttcctg agcgacaata gtttccgcct caaccgctgc
    21721 ggctttacgc tgcgccaggt tgtgcgaaat gatgctttgc agatcgtcca cactataaag
    21781 ataagcattc gccagtttgc caacttccgg ctcaacatcg cgcggaacgg caatatccac
    21841 caacagcatt ggttggttgc gacggctttt taatgcgcgc tccaccatgc ctttcccgat
    21901 aatcggtaac gggctggcgg tggaactgat gatgatatcg gcttcgcgca gacgttcgtc
    21961 gatctcactc agggcaatca cttccgcgcc aacttcatct gccagaattt gggcacgttc
    22021 gcgagtgcgg ttggcgataa tcatcttctg tactttatgt tcgcgcagat gacgcgctac
    22081 cagctcgatg gtttcgcccg cgcctaccag caacactgtg accgtagaga gcgattcaaa
    22141 gatctgccgc gccagcgtac aagccgcaaa agcgacagac acagcactgg caccgatatc
    22201 tgtttcagtg cgaacgcgtt tcgctacaga gaaagatttc tggaacatgc gttccagttc
    22261 gctggccttc atatgacctt tttgcgaatc ggcaaacgct tttttaacct gaccgaggat
    22321 ctgcggctcc ccaagaacca gtgaatctag gccgctggca acacgcatta aatggctaac
    22381 cgcgtcgtta tcctgatgcc agtagaggct tttacgcaga tcttcttcat taagattgtg
    22441 ataatcgcaa agccagcgga ttaacgcctc ttgcaggtta tcctgctctt caacgctaag
    22501 ataaagttcc gtgcggttgc acgtcgacag caccacgccg ccctgcacca tcggctgcgc
    22561 aagcaggctg tcaagcgcct gatcgagctt atccggcgaa aacgatacac gttctcgcag
    22621 cgatacaggt gccgttttat ggttgatacc gagtgctaaa agggtcatgt ctgcgggaaa
    22681 taataccaac gttgataggg ttagtctgct tgcatcatac aggatgcgta agatcaataa
    22741 aagagagcgc ccccttttgg agtaattgcc gaaagccgtt agattctggc aattaagaca
    22801 acttgaacat agacgatagc ggacggtaac gctagcatta agggttataa ctgcaacgta
    22861 tctcaaggac ttgtcatcac tatgcccctg cccgattttc gtcttatccg cctgctaccg
    22921 ctggctgctc ttgtgctcac tgcctgttcc gttaccacgc ccaaaggtcc tggcaaaagc
    22981 ccggattcgc cacaatggcg tcagcatcag caagacgtgc gcaatcttaa tcagtatcag
    23041 actcgcggcg cgttcgctta tatttctgac caacaaaaag tgtacgcccg ctttttctgg
    23101 cagcaaaccg gccaggatcg ctaccgtctg ctgctcacta acccattggg cagcacggaa
    23161 ctggagctga atgctcaacc gggtaacgtg cagttagtcg acaataaagg tcagcgttat
    23221 accgccgatg acgccgaaga gatgattggc aaattgaccg gaatgccaat tccgctcaac
    23281 agcttgcgcc agtggatttt aggtttaccg ggtgatgcaa ccgactacaa actggacgac
    23341 cagtaccgcc tgagcgaaat tacctacagc cagaatggca aaaactggaa ggttgtttat
    23401 ggtggttatg acaccaaaac gcaacctgcg atgccagcca atatggaact caccgacggt
    23461 ggtcaacgca tcaagttaaa aatggataac tggatagtga aataatgcgg acacagtggc
    23521 cctctccggc aaaacttaat ctgtttttat acattaccgg tcagcgtgcg gatggttacc
    23581 acacgctgca aacgctgttt cagtttcttg attacggcga caccatcagc attgagcttc
    23641 gtgacgatgg ggatattcgt ctgttaacgc ccgttgaagg cgtggaacat gaagataacc
    23701 tgatcgttcg cgcagcgcga ttgttgatga aaactgcggc agacagcggg cgtcttccga
    23761 cgggaagcgg tgcgaatatc agcattgaca agcgtttgcc gatgggcggc ggtctcggcg
    23821 gtggttcatc caatgccgcg acggtcctgg tggcattaaa tcatctctgg caatgcgggc
    23881 taagcatgga tgagctggcg gaaatggggc tgacgctggg cgcagatgtt cctgtctttg
    23941 ttcgggggca tgcctcgttt gccgaaggcg ttggtgaaat actaacgccg gtggatccgc
    24001 cagagaagtg gtatctggtg gcgcaccctg gtgtaagtat tccgactccg gtgattttta
    24061 aagatcctga actcccgcgc aatacgccaa aaaggtcaat agaaacgttg ctaaaatgtg
    24121 aattcagcaa tgattgcgag gttatcgcaa gaaaacgttt tcgcgaggtt gatgcggtgc
    24181 tttcctggct gttagaatac gccccgtcgc gcctgactgg gacaggggcc tgtgtctttg
    24241 ctgaatttga tacagagtct gaagcccgcc aggtgctaga gcaagccccg gaatggctca
    24301 atggctttgt ggcgaaaggc gttaatcttt ccccattgca cagagccatg ctttaagccg
    24361 ggcaagctga gtttcggtga caacgtcacc ttgttccaga cgttgcatcg cgctctttaa
    24421 tacaccgcct ggaaaggatc atgcctggcc cgcacagttt tcggcagatt ctttccacca
    24481 atggacgcat gcctgaggtt cttctcgtgc ctgatatgaa gctttttgct ggtaacgcca
    24541 ccccggaact agcacaacgt attgccaacc gcctgtacac ttcactcggc gacgccgctg
    24601 taggtcgctt tagcgacggc gaagtcagtg tacaaatcaa cgaaaatgta cgcggtggtg
    24661 atattttcat catccagtcc acttgtgccc ctactaacga caacctgatg gaattagtcg
    24721 ttatggttga tgccctgcgt cgtgcttccg caggtcgtat caccgctgtt atcccctact
    24781 ttggctatgc gcgccaggac cgtcgcgtcc gttccgctcg tgtaccaatc actgcgaaag
    24841 tggttgcaga cttcctctcc agcgtcggtg ttgaccgtgt gctgacagtg gatctgcacg
    24901 ctgaacagat tcagggtttc ttcgacgttc cggttgataa cgtatttggt agcccgatcc
    24961 tgctggaaga catgctgcag ctgaatctgg ataacccaat tgtggtttct ccggacatcg
    25021 gcggcgttgt gcgtgcccgc gctatcgcta agctgctgaa cgataccgat atggcaatca
    25081 tcgacaaacg tcgtccgcgt gcgaacgttt cccaggtgat gcatatcatc ggtgacgttg
    25141 caggtcgtga ctgcgtactg gtcgatgata tgatcgacac tggcggtacg ctgtgtaaag
    25201 ctgctgaagc tctgaaagaa cgtggtgcta aacgtgtatt tgcgtacgcg actcacccga
    25261 tcttctctgg caacgcggcg aataacctgc gtaactctgt aattgatgaa gtcgttgtct
    25321 gcgataccat tccgctgagc gatgaaatca aatcactgcc gaacgtgcgt actctgaccc
    25381 tgtcaggtat gctggccgaa gcgattcgtc gtatcagcaa cgaagaatcg atctctgcca
    25441 tgttcgaaca ctaatcgaac ccggctcaaa gacccgctgc ggcgggtttt tttgtctgta
    25501 atatccattt gtatgaccta tgcctccttc acctgccatt tagttgacag atgatgcgct
    25561 cacggatgaa acattattgt gaacaaaata ttttcctcac atgtgatgcc tttccgcgct
    25621 ctgatcgacg cttgctggaa agaaaaatat actgccgcac ggtttacccg tgacctgatt
    25681 gccgggataa ccgtcgggat tattgctatc ccgctggcga tggcgttggc tattggtagt
    25741 ggtgtggcac cccagtacgg tttatatacc gcagctgttg cggggattgt cattgctctg
    25801 acgggtgggt cacgctttag cgtttccggt ccgactgcgg catttgtggt aattctctat
    25861 cccgtgtcgc aacagtttgg actggcagga ctgctggttg cgaccttgct gtcggggatt
    25921 tttttgattc tgatgggtct ggcacgcttt ggtcgcctga ttgagtatat tccggtttcc
    25981 gtcaccttag gtttcacctc gggtatcggg atcaccatcg gtaccatgca gattaaagat
    26041 tttctcggtc tgcaaatggc ccatgtcccg gaacattatc tacaaaaagt cggcgcatta
    26101 tttatggcgc tgccgaccat taatgtgggt gatgctgcca ttggcattgt gacgctaggt
    26161 attcttgttt tctggccgcg tctgggcatt cgtttacccg gtcaccttcc ggccttgctg
    26221 gctggttgcg cggtgatggg gattgttaac ctgctcggcg gacatgttgc taccatcggt
    26281 tcgcaattcc actacgtcct ggccgatggt tctcagggta acggtattcc gcaactgctg
    26341 ccgcaactgg tgctgccgtg ggatctgcct aattcagaat tcacgctaac ctgggattct
    26401 attcgcacac tgctgcctgc ggcattctca atggcaatgc tcggcgcaat cgaatctctg
    26461 ctctgcgccg tggtgctgga tggtatgacc gggacgaaac acaaggcgaa tagcgaactg
    26521 gttggacagg gactggggaa tatcatcgct ccgttctttg gtggcattac cgctacagct
    26581 gccatcgcgc gttctgccgc taacgtccgt gccggggcaa cttcccctat ctcggcggtg
    26641 atccactcta ttctggttat tcttgccctg ctggtactgg caccgctgct ctcctggctg
    26701 ccgctttccg ccatggctgc cctgctgttg atggtggcgt ggaacatgag tgaagcgcac
    26761 aaagtggtcg acttgctgcg tcatgcgccg aaagatgaca tcattgtcat gctgctgtgc
    26821 atgtcgctga ccgtgctgtt tgatatggtt attgccatca gcgtggggat cgtgctggca
    26881 tcgctgctgt ttatgcgtcg tatcgcacgt atgactcgcc tggcaccggt agtcgtagat
    26941 gttccagacg atgtcctggt tctgcgcgtt attggcccgc tgttttttgc tgctgctgaa
    27001 ggcttgttca cggacctgga gtcacgtctt gaaggcaaac ggattgtgat tctgaagtgg
    27061 gatgcggttc cggtacttga tgctggtggt cttgatgcgt tccagcgttt tgtgaagcgt
    27121 ctgcccgagg gatgtgaact gcgcgtgtgc aacgtggaat tccagccact gcgcactatg
    27181 gctcgcgctg gcattcaacc gatcccggga cgcctggcgt tcttcccgaa tcgtcgcgcg
    27241 gcgatggcgg atttataagt acgagattga ccagtcagcg caaactgact ggtcagcaaa
    27301 ctgcattatt tgttagctat gacggcgatt atcgctacgg cggcaacgtt tgtcatagtg
    27361 gcgaacccac cagtaacggt cgcacacctg ttcatgcccg ccaacacggg cacccgccag
    27421 ccagagaatg gcaccgacga aaatacttag cactgcacca tgtgcgaaat actggggcat
    27481 attaaactgt ggtaactggt tgaggattga ataccccacg ccgaccacca ttaccaccag
    27541 acccaacccc atgagcacgt taccgagtaa cgaagcgttt ttgcgtttca tatgtcacct
    27601 ccggaacttt ctgggttgta acagggaata cccctcttcc ttatgtgtaa agtatagaca
    27661 accccctgtg ggttatgtgc gggcgtgatc acaattacaa cccttatttc aacaaaactt
    27721 tacaaataaa cgcctgaaca ccttgacttt ctaatagtcc actcatgcaa ttattacgtt
    27781 atctgcattt gatacgcagt tttttgtcaa gcggggccgc aaccagtaaa ctacgcgcca
    27841 gttatgtaca cactcaggac aaaaaaacgt gacgattaaa ttgattgtcg gcctggcgaa
    27901 ccccggtgct gaatacgccg caacgcgaca taatgctggt gcctggttcg ttgacttact
    27961 ggcagagcgt ttgcgcgctc cgctgcgcga agaggctaaa ttctttggtt atacttcgcg
    28021 agtcactctt ggaggcgaag atgtccgcct gttagtcccg actacattta tgaatctcag
    28081 cggcaaagcc gttgcggcga tggccagttt tttccgcatt aatccggacg aaattctggt
    28141 ggcccacgac gaactggatc tgcctcctgg cgtcgccaaa tttaaattgg gcggtggcca
    28201 tggtggtcac aatggactga aagacatcat cagtaaattg ggtaataacc ctaactttca
    28261 ccgtttacgc atcggaatcg gtcatccggg cgataaaaat aaagttgtcg gttttgtgtt
    28321 aggcaaaccg cctgttagtg aacagaagtt aattgatgaa gccatcgacg aagcggcgcg
    28381 ttgtactgaa atgtggttta cagatggctt gaccaaagca acgaaccgat tgcacgcctt
    28441 taaagcgcaa taagtcgttg tctgcggcat ttttgccgag tgccgtgtat aataggcaaa
    28501 gttatttcca tttctgcaat ctgttagcaa taacaggttg attattaaga tattaaggtg
    28561 atttaaatca tgggattcaa atgcggtatc gtcggtttgc ccaacgtcgg gaaatctacc
    28621 ctgttcaacg cgctgaccaa agccggtatt gaagcggcca actttccatt ctgcaccatt
    28681 gagccgaaca caggcgtcgt accaatgcct gaccctcgcc tggatcaact ggctgaaatc
    28741 gtaaaaccgc aacgtacgct tcccacgacc atggaatttg tcgatatcgc cggtctggta
    28801 aaaggcgcat cgaaaggcga aggtctgggt aaccagttcc tgaccaacat ccgtgaaacc
    28861 gaagcgatcg gtcacgttgt tcgctgcttt gaaaatgaca acatcattca cgtttccggc
    28921 aaagttaacc cggctgacga tattgaagtt atcaacaccg aactggcgct ggcagacctc
    28981 gacacctgcg aacgtgcgat tcatcgcgta cagaagaaag ccaaaggtgg cgataaagac
    29041 gcgaaagctg agctggcggt cctggaaaaa tgcctgcccc agttggaaaa tgcaggtatg
    29101 ctgcgcgcgc tggatttaag cgctgaagag aaagcggcta ttcgttacct gagcttcctg
    29161 acgctgaaac caacaatgta catcgccaac gtcaacgaag acggttttga aaacaaccca
    29221 tatcttgacc aggtgcgtga aatcgcggcg aaagaaggtt ctgttgtggt tccggtttgt
    29281 gctgctgttg aagcagacat tgccgaactg gacgacgaag aacgtgacga gtttatgcag
    29341 gagcttgggc tggaagagcc gggcctgaac cgtgtgatcc gtgccggtta taagctgctg
    29401 aacctgcaaa cttacttcac cgctggggtg aaagaagtgc gtgcatggac cattccggtt
    29461 ggagcaaccg cgccgcaggc agcgggcaaa atccatactg attttgaaaa aggctttatc
    29521 cgtgcacaaa ccatctcgtt tgaagatttc atcacttaca aaggtgaaca aggcgcgaaa
    29581 gaagcaggca aaatgcgtgc agaaggtaaa gattacatcg ttaaagatgg cgatgtgatg
    29641 aacttccttt tcaacgtcta attaatttgt tgtctcatga ggtttcgcta aatctcatga
    29701 agagcgcaaa agtatatata atccacgcaa ttgcgtggat ttttcatttc accgagtcct
    29761 aatcattctt gtaacatcct acacttttgt gcttattgcc tacagtgaac cacgagaata
    29821 attaaagaca tcagtgtcag atgacggata tctcattagt agtgatataa cgtaagtttt
    29881 ggtccactac acatcacccc cttcacagct gatcaatatt gatcacgctc caatatgctg
    29941 gcgagaaaag gacttacctg aagggtaata tcccgcagat aaagtccccg aataacgtga
    30001 ttacgtgcga tcctggcaag atgaaaatga taaccagtag cacaataaca acgataggcc
    30061 agaaaaccca ttgaccaaaa atgaatatcg atgttttttg ctttttctta ttagtaatgc
    30121 aaaaatggcg catattgttt ttgttatgca gatacatca
//
LOCUS       DAZFJF010000052        30008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFJF010000052 DAZFJF010000000
VERSION     DAZFJF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.052"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..181)
                     /gene="hokA"
                     /locus_tag="ACHKAU_002499"
     CDS             complement(29..181)
                     /gene="hokA"
                     /locus_tag="ACHKAU_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO5531548.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(506..2575)
                     /gene="glyS"
                     /locus_tag="ACHKAU_002500"
     CDS             complement(506..2575)
                     /gene="glyS"
                     /locus_tag="ACHKAU_002500"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO5531549.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2585..3496)
                     /gene="glyQ"
                     /locus_tag="ACHKAU_002501"
     CDS             complement(2585..3496)
                     /gene="glyQ"
                     /locus_tag="ACHKAU_002501"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO5531550.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3591..3890)
                     /gene="ysaB"
                     /locus_tag="ACHKAU_002502"
     CDS             complement(3591..3890)
                     /gene="ysaB"
                     /locus_tag="ACHKAU_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO5531551.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            4065..5060
                     /gene="wecH"
                     /locus_tag="ACHKAU_002503"
     CDS             4065..5060
                     /gene="wecH"
                     /locus_tag="ACHKAU_002503"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO5531552.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5102..5539)
                     /gene="yiaA"
                     /locus_tag="ACHKAU_002504"
     CDS             complement(5102..5539)
                     /gene="yiaA"
                     /locus_tag="ACHKAU_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO5531553.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5585..5926)
                     /gene="yiaB"
                     /locus_tag="ACHKAU_002505"
     CDS             complement(5585..5926)
                     /gene="yiaB"
                     /locus_tag="ACHKAU_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO5531554.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(6095..7549)
                     /gene="xylB"
                     /locus_tag="ACHKAU_002506"
     CDS             complement(6095..7549)
                     /gene="xylB"
                     /locus_tag="ACHKAU_002506"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO5531555.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7621..8943)
                     /gene="xylA"
                     /locus_tag="ACHKAU_002507"
     CDS             complement(7621..8943)
                     /gene="xylA"
                     /locus_tag="ACHKAU_002507"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO5531556.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            9309..10301
                     /gene="xylF"
                     /locus_tag="ACHKAU_002508"
     CDS             9309..10301
                     /gene="xylF"
                     /locus_tag="ACHKAU_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5531557.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            10379..11920
                     /gene="xylG"
                     /locus_tag="ACHKAU_002509"
     CDS             10379..11920
                     /gene="xylG"
                     /locus_tag="ACHKAU_002509"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO5531558.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11898..13079
                     /gene="xylH"
                     /locus_tag="ACHKAU_002510"
     CDS             11898..13079
                     /gene="xylH"
                     /locus_tag="ACHKAU_002510"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO5531559.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            13157..14335
                     /gene="xylR"
                     /locus_tag="ACHKAU_002511"
     CDS             13157..14335
                     /gene="xylR"
                     /locus_tag="ACHKAU_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO5531560.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14443..15267)
                     /gene="bax"
                     /locus_tag="ACHKAU_002512"
     CDS             complement(14443..15267)
                     /gene="bax"
                     /locus_tag="ACHKAU_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO5531561.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15587..17617
                     /gene="malS"
                     /locus_tag="ACHKAU_002513"
     CDS             15587..17617
                     /gene="malS"
                     /locus_tag="ACHKAU_002513"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5531562.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17795..19048
                     /gene="avtA"
                     /locus_tag="ACHKAU_002514"
     CDS             17795..19048
                     /gene="avtA"
                     /locus_tag="ACHKAU_002514"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO5531563.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(19200..19673)
                     /gene="ysaA"
                     /locus_tag="ACHKAU_002515"
     CDS             complement(19200..19673)
                     /gene="ysaA"
                     /locus_tag="ACHKAU_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5531564.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19775..20623)
                     /gene="yiaJ"
                     /locus_tag="ACHKAU_002516"
     CDS             complement(19775..20623)
                     /gene="yiaJ"
                     /locus_tag="ACHKAU_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO5531565.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20824..21822
                     /gene="yiaK"
                     /locus_tag="ACHKAU_002517"
     CDS             20824..21822
                     /gene="yiaK"
                     /locus_tag="ACHKAU_002517"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO5531566.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21834..22301
                     /gene="yiaL"
                     /locus_tag="ACHKAU_002518"
     CDS             21834..22301
                     /gene="yiaL"
                     /locus_tag="ACHKAU_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO5531567.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            22419..22892
                     /gene="yiaM"
                     /locus_tag="ACHKAU_002519"
                     /pseudo
     CDS             22419..22892
                     /gene="yiaM"
                     /locus_tag="ACHKAU_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
     gene            22895..24172
                     /gene="yiaN"
                     /locus_tag="ACHKAU_002520"
     CDS             22895..24172
                     /gene="yiaN"
                     /locus_tag="ACHKAU_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO5531568.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            24185..25171
                     /gene="yiaO"
                     /locus_tag="ACHKAU_002521"
     CDS             24185..25171
                     /gene="yiaO"
                     /locus_tag="ACHKAU_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO5531569.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            25175..26671
                     /gene="lyxK"
                     /locus_tag="ACHKAU_002522"
     CDS             25175..26671
                     /gene="lyxK"
                     /locus_tag="ACHKAU_002522"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO5531570.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26668..27330
                     /gene="ulaD"
                     /locus_tag="ACHKAU_002523"
     CDS             26668..27330
                     /gene="ulaD"
                     /locus_tag="ACHKAU_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5531571.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            27323..28183
                     /gene="sgbU"
                     /locus_tag="ACHKAU_002524"
     CDS             27323..28183
                     /gene="sgbU"
                     /locus_tag="ACHKAU_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO5531572.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            28177..28872
                     /gene="araD"
                     /locus_tag="ACHKAU_002525"
     CDS             28177..28872
                     /gene="araD"
                     /locus_tag="ACHKAU_002525"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5531573.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28904..29005)
                     /locus_tag="ACHKAU_002526"
                     /pseudo
     CDS             complement(28904..29005)
                     /locus_tag="ACHKAU_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            29009..29272
                     /locus_tag="ACHKAU_002527"
                     /pseudo
     CDS             29009..29272
                     /locus_tag="ACHKAU_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29219..29959)
                     /gene="yiaT"
                     /locus_tag="ACHKAU_002528"
     CDS             complement(29219..29959)
                     /gene="yiaT"
                     /locus_tag="ACHKAU_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO5531574.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
BASE COUNT         7417 a         7690 c         7500 g         7401 t
ORIGIN      
        1 ggcgggaata acttcccgcc gaagactctt actcttttaa attgcaggct aaaaatgccg
       61 ccagctcata actcccctgt ttaatatgca attcacacag tgaatctctt atcatccagg
      121 tgaaaaataa aagcgtgaaa caaatcacta ttaaagaaag taatctatat ttctgcggca
      181 ttcccagccc cctgtgttga tttccaacga gtaagaggct aaccttatgg tgttcaggca
      241 tagggtagcc tcactttgat ttatagtcag gtggggcttt tctctgtctg cctttcggtg
      301 aatacctgag acaaacagtc tcaagcaccc gtggctattc tagcttaata agtttgtttc
      361 ttctccttga tataatccta aaaaacctca taaaattaat atatgagata atctttattc
      421 agcagaagat tattaaaggt tgctgtatta tttagcgata ataaaaaaag cctgccagat
      481 ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca acgcgcagga
      541 acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct ttgtcatcaa
      601 ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct gccagttcga
      661 ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca cgtagcacca
      721 caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca ttcacgcggt
      781 cgctcagcac ttcgtcagat ttcgccaaaa tgttggagac acgcttgttc gccgccgcca
      841 gtgcagcagc tgcttcaagg gtacggaagt gcgataccgc tttcatgcgg gcatcgaaat
      901 cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg gtgtaacctt
      961 cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca tcaaccacgt
     1021 tggcgttagt cagcttatcg ccatacagac gcactgcttc ttcggtcagc gtttgcagat
     1081 caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca cgacgcagcg
     1141 caaacgggtc tttgtcgcct ttcggatgct ggccgatacc gaagataccc gccagagtat
     1201 ccatcttgtc agcaatcgcc agcgcacagg ctaccgggtt agacggcagg tcatcaccgg
     1261 caaagcgcgg ctgatactgc tcattcagcg ccaccgcgac atcttccgct tcgccatcgt
     1321 gacgcgcata gtgcatcccc ataacgccct gggtgtcggt gaactcgaag accatgttgg
     1381 tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg tcagcgccaa
     1441 tctgttcagc aatccagcca gccagcgcct ggatgcggtc agttttgtcg cgcagcgtac
     1501 ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc agacgttttt
     1561 tacggtcggt attgaagaag aactcggcat ccgccagacg tggacgaacg actttctcgt
     1621 taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag atgaagttcg
     1681 gcagcagttt gccatcgttc gcatacaccg ggaagtattt ctggtcacct ttcatggtgt
     1741 aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg gtcagaacga
     1801 ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag tcagcgttac
     1861 cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta cgttcttcgt
     1921 aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg gcgttgtcga
     1981 tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg gactgaatgc
     2041 ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg tgcaccggac
     2101 gcacgaagtg cacgtcgctt gcgccccaac gcatcagttt cgggatcggc agtttcgcca
     2161 gagaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc acatgggcgc
     2221 gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca acggtaatac
     2281 cgcaaccacg cgcccaacct tctgccgctt tgctcggttt gccttcagcg tcgaacgcct
     2341 gggcaatcgc cgggccgcgt ttttcgattt cacgatccgg ttgcgcttcc gccaggttag
     2401 ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg tgtgcgaggc
     2461 cagcgttatc cagctccgca gtaaaattcg cagcaaagga ctcagccagg ctgcgcagtg
     2521 cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca gacatagccg
     2581 cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa gcgtagtatg
     2641 cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga cgctcggtga
     2701 cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc agaatacgct
     2761 cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct tctttctcgt
     2821 actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg taagtggact
     2881 gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc gggccgtcgc
     2941 tccagaccag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga cgttccagac
     3001 cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc tggaagtaag
     3061 tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc caggcaccca
     3121 gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc gggtccatgc
     3181 ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat ggcttaatga
     3241 ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag cgaccatcgg
     3301 tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc agcgcgcgca
     3361 gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt tgaacaatgg
     3421 tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg aaggtcctgg
     3481 tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg aagggatcag
     3541 tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc ttaacgcatg
     3601 gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc tttacggaag
     3661 gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac gtcgattttt
     3721 tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc catactcaac
     3781 gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg tgtagaacaa
     3841 cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat taaattaccg
     3901 tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat cttacgaatt
     3961 tccttaagcc tctataagct gtagtgataa aactcagatt attccagtgc gccaaccaaa
     4021 tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag cccaaaattt
     4081 actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt cacaccacta
     4141 cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc gccaatgttc
     4201 tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc tatctctttt
     4261 ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt ctgatttttt
     4321 atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg gagttagcgc
     4381 tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc gcgattgcgg
     4441 tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa atgttgctgg
     4501 tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag aaaattgacg
     4561 gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac tacattctgt
     4621 atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca ctgtcgtggg
     4681 tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca ttatatgaat
     4741 tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg atggttttta
     4801 tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt accattcgcg
     4861 gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc ttgattatcc
     4921 atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat attatttgga
     4981 tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa cgaatcgaca
     5041 gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg gcttacgtga
     5101 attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt attccggtat
     5161 cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa gccagtccat
     5221 aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc agtaatgcaa
     5281 cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc ggtatgcctt
     5341 catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct aacaccagta
     5401 cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc cctaacagat
     5461 aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc ggtgaatagg
     5521 ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa gagctttatg
     5581 ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa aaggcgatgg
     5641 ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata aacaagagac
     5701 cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc agttgcccga
     5761 gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac acgccaagaa
     5821 aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga taaaccagcg
     5881 ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg atctccatat
     5941 ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg ccattaacaa
     6001 atgattttca gaataaattc atactaaata ttaattaatt agtgagatat atagatgtga
     6061 attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc agaagttgct
     6121 gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc tgcgcatctg
     6181 gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat ttctctggat
     6241 tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc ccccccgtac
     6301 ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac tcactacgcg
     6361 ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc acgacatcca
     6421 tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt tcattggggc
     6481 catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg tgtggtgtac
     6541 gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca gcctgttgag
     6601 ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcgacc caatccagac
     6661 acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc gcatggcaaa
     6721 agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca aaatagaccc
     6781 ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca ccaactgcac
     6841 cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc cacgctttcg
     6901 caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg ggcatctggt
     6961 cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt gcgacatcca
     7021 gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc atacgcagac
     7081 gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc ggctcatgcc
     7141 gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg ccggtaatca
     7201 ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag cgcccgtcgt
     7261 tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt gctccatgca
     7321 tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg cccagagctt
     7381 tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac cagagtggat
     7441 gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc tgctcgttga
     7501 gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata tcgatcgttc
     7561 cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca cagttagccg
     7621 ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc gaccactctg
     7681 atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca gtgacatttg
     7741 gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct gcgcgatgcg
     7801 tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca ctgccatcgt
     7861 atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt gacgacgtac
     7921 tttggcatcg aagttcagac caccggtggt gaaaccacct gctttgagaa tttcatacat
     7981 caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc ccagttgcgc
     8041 atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg tggctatttc
     8101 atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca gtttaatctc
     8161 tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt aatcatattg
     8221 atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctgga agccgatttt
     8281 atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct cctgacgcaa
     8341 gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat agttttcacc
     8401 gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc agctgaagac
     8461 ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa agcagttagc
     8521 ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga catcaaccat
     8581 ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa catccacatc
     8641 gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga catctgcttt
     8701 acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa acgcccccac
     8761 accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg caaaacgcaa
     8821 gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac ggaatgctaa
     8881 cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa aataggcttg
     8941 catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt aatattgggc
     9001 actccctttc agttgctcaa ttatgttatt tcacactgct attgagataa ttcacaagtg
     9061 tgcgctcgct cgcaaaataa aatgggatga tgaaactggg taattcctcg aagataaaaa
     9121 tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa gaaataaacc
     9181 aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag ttgctaaaaa
     9241 ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac tacagaaggc
     9301 cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt ctgcttacca
     9361 acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat ctccgtcttg
     9421 aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc ggcgcgaaag
     9481 tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt gaaaacatga
     9541 taaaccgggg tgttgatgtt cttgtcatta ttccgtataa cggtcaggta ttaagtaacg
     9601 ttgtaaaaga agccaaacaa gaaggtatta aagtattagc ttacgatcgt atgattaacg
     9661 atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa ctgcaggcaa
     9721 aagccctggt cgatattgtt ccgcaaggta attacttcct gatgggcggc tcgccggtag
     9781 ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct tacgttgatt
     9841 ccggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg gaaaacgcat
     9901 tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct gtagttgcct
     9961 caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt ttatcaggga
    10021 aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt gctgccggta
    10081 cgcaaactat gacggtgtat aaacctatta cgttgttggc aaatactgcc gcagaaattg
    10141 ccgttgagtt gggcaatggt caggaaccaa aagcagatac cacactgaat aatggcctga
    10201 aagatgtgcc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac atcaaagaca
    10261 cggtagttaa agacggattc cacaaagaga gcgagctgta agcgttacgc cccggcgcgg
    10321 agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg gagtcgttat
    10381 gccttatcta cttgaaatga agaatattac caaaaccttc ggcagtgtga aggcgattga
    10441 taacgtctgc ttgcggttga atgctggcga aatcgtctca ctttgtgggg aaaatgggtc
    10501 tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct cctacgaagg
    10561 cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata ccgaacgcaa
    10621 aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg tgctggaaaa
    10681 tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc tgatgacgct
    10741 acgctgtcag aagctgctcg cacaggtcag tttatccatt tcacctgata cccgcgttgg
    10801 cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta ataaacaggt
    10861 gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa cgtcggtttt
    10921 actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata tttcgcacaa
    10981 actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg gacagcacat
    11041 tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga tggtcgggcg
    11101 agagttaacc gcgctttacc ctaatgaacc acataccacc ggagatgaaa tattacgtat
    11161 tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta atgatgtctc
    11221 gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg ccggacgtac
    11281 cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa aaatttatat
    11341 tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcgcccagg ggattgcgat
    11401 ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg gtaaaaatat
    11461 taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg acgcggaaga
    11521 gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt cccccgatct
    11581 tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgctc gctgtctgtt
    11641 acttaacccg cgcatcctca ttcttgatga acctaccagg ggtatcgata ttggcgcgaa
    11701 atacgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg ttattgtcat
    11761 ctcttccgaa ttacctgaag tgctcggcct tagcgatcgg gtactggtga tgcatgaagg
    11821 gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga tggaagccgc
    11881 attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg gccgtaccga
    11941 catccggtgg cttttccggg ctgaaatcac tgaatttgca ggtcttcgtg atgattgcag
    12001 ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac ttaagcgccc
    12061 gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg gtaggaatgg
    12121 tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg gggctattag
    12181 gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt accatcattg
    12241 tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc gcgtaccgca
    12301 aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc atactcattg
    12361 gcatcaccaa cggcacgact gtttccccca ccagcgccgc gatgtcacaa attgggcaaa
    12421 gctatctacc tgccagcact ggcttcatca ttggcgcgct tggcttaatg gcttttgttg
    12481 gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttgcagtct ccggcctcta
    12541 ccgcagtagt cggtcgccag gctttaaccg ctatcatcgt attaggcgca atctggctgt
    12601 tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacattgctg ttactcggcg
    12661 gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc ggcggcaatc
    12721 tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc gtgttcgcga
    12781 ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt ggcgctggtt
    12841 caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg attggcggca
    12901 ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca tttatcatgg
    12961 cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag tatatcgtta
    13021 aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc cgttcttgat
    13081 tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt ttgcatgatt
    13141 cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt tcaatgccaa
    13201 taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg catcacaatc
    13261 agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa tcaaggactg
    13321 gttaggagat ggcgtcattg ccgacttcga cgacaaacag atcgagcaag cgctggctga
    13381 tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa gttacccacc
    13441 cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt tgcatttaaa
    13501 agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg gcaaacgttg
    13561 ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt atcgcggagt
    13621 ggtttatcag gggttagaaa ccgcgccaga gaactggcaa catgcgcaaa atcggctggc
    13681 agactggcta caaacgctgc caccgcaaac cgggattatt gccgttactg acgcccgggc
    13741 gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa aattatgcgt
    13801 gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc tttcttcggt
    13861 cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc gattattaga
    13921 taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca ttgaacggcg
    13981 ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc attacattcg
    14041 taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg ggatctcgcg
    14101 ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg caatgattca
    14161 tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt cgatcaatga
    14221 gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt ttaaaaaagc
    14281 atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt tgtagcgatg
    14341 atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc gagcaaaagc
    14401 cttatccgac cgacaaatca gataaggctc aaggaaatgc aattacatat gcgccgcgat
    14461 taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct tccctttggt
    14521 cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat ccgctttacg
    14581 cagctgcgca cgcgatttac ggaatgaaga gtaagccgga tgcgtattga ggttagtgac
    14641 ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca ctttacccgg
    14701 cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttgt tgttgcgcgc
    14761 cagctttgac gttccccaac cgctttctgc tgcagccatc gtcgccacca tactggtggg
    14821 gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat taccagacca
    14881 cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag gtgaccattg
    14941 gccctgatac tgttttgaaa tcagccagtt acgttccgca gtaatggctg cattttggct
    15001 ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc cggaagggta
    15061 ttttcgcaaa tcaggaagtg aattactctt tgcactattg cgagaatact cttgtttact
    15121 gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg ttgttgtgtg
    15181 cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa tcatcgcccc
    15241 atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat cattccaaca
    15301 ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa tagctgtcaa
    15361 gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga ttcagattgg
    15421 cgctaaatcg cagaaaatgt ggggtttatc gcaaaattca gccgtttttt gcgcgagatc
    15481 gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt gtttggctta
    15541 tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccgccatga aactcgccgc
    15601 ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt ctccggggtt
    15661 ccctgccttt agtgaacagg gaacggaaac atttgtcagc cacgcgcagt tgcccaaagg
    15721 tacgcgtcca ctaacgctaa attttgacca acagtgctgg cagcctgcgg atgcgataaa
    15781 actcaatcag atgctttccc tgcaaccttg tagcaacacg cagcctcaat ggcgattgtt
    15841 cagggacggc gaatatacgc tgcaaataga cacccgctcc ggcacgccaa cattgatgat
    15901 ttccatccag aacgccgccg aaccggtagc aagccaggtc cgtgtatgcc cgaaatggga
    15961 tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg ccgtacggga
    16021 ttattacagc cagcaaattg cgatagtgaa gaacggtcaa ataacgttac aacccgctgc
    16081 caccagcaac ggtttactcc tgctggaacg ggcagaaact gacgcctctg cccctttcga
    16141 ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg gcgatcccag
    16201 taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca cttttcacgg
    16261 cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg gcgttaatgc
    16321 tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg gtacaaaagg
    16381 cgatttcccg cattatgcct accacggtta ttacacacag gactggacga atcttgatgc
    16441 caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc agcgcggtat
    16501 tcgtattctc tttgatgtcg tgatgaacca caccggctat gccacgctgg cggatatgca
    16561 ggagtatcag tttggcgcgt tatatctttc tggtgacgag gtgaaaaaaa cgctgggtga
    16621 acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta acgattacat
    16681 taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga tccgtaccga
    16741 tatcggcgat tacgacaatc ctggattcga cgatctcacc atgtcgctag cctttttgcc
    16801 ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata aaaacaaaac
    16861 ggatacccac gctaaagcca tcgacggctt tacccctcgc gattacttaa cccactggtt
    16921 aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg ccaaacatgt
    16981 tgagttgccc gcctggcagc aactgaaaac cgaagccagc gccgcgcttc gcgaatggaa
    17041 aaaagctaac cccgacaaag cattagatga caaacctttc tggatgaccg gtgaagcctg
    17101 gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga tgatcaattt
    17161 cgattatcag gagcaggcgg cgaaagctgt cgattgtatt gcgcagatgg atatgacctg
    17221 gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct cgtcgcatga
    17281 tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat tagcgccagg
    17341 cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta caggttctga
    17401 tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta aatctgccgc
    17461 caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg caattggcgc
    17521 gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg agcatggcga
    17581 cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc ccgttcgtca
    17641 gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt tgcaggataa
    17701 agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg ttattgttac
    17761 cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt ttggtgacaa
    17821 atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg gtttacgcac
    17881 ccctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa tgcaggacta
    17941 cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg cactgtgcaa
    18001 ttacgacggt ccacagggga aaacggagct actcacactg cttgccggaa tgctgcgcga
    18061 gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca gccagagcgc
    18121 gtttttctac ttatttaatc tgtttgccgg acgccgtgcc gatggtcggg tcaaaaaagt
    18181 gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg aagaagatct
    18241 gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta aataccacgt
    18301 cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct cccggccgac
    18361 gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg cgctggcgaa
    18421 tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc cgggtatcat
    18481 cttcagtgaa gcgcgcccgc tatggaatcc gaatatcgtg ctgtgcatga gtctttccaa
    18541 gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa tcatcaccgc
    18601 catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc cggcgatgat
    18661 gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca tcaaaccgtt
    18721 ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac cggaaaatcg
    18781 ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta aggatttgcc
    18841 cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga tggtgccggg
    18901 gcacaacttc ttcccagggc tggataaacc gtggccgcat acgcatcaat gtatgcgcat
    18961 gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg cggaagagat
    19021 agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct tatcaggcct
    19081 acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa ggcgtttacg
    19141 ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg aaaaacgttt
    19201 caaacaggct gctgccgtag ccgatgccgc tgcactttct cgacatccat gcactgcaac
    19261 gcctttgtcg ggcaggcttc aacacaggcc ggtcccgtct cccgatgcca gcacagatcg
    19321 cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc aaacgggcaa
    19381 gccagcatac agcttttaca gccaatgcaa cgtgtttgtt caacgaaaat atgcccatgt
    19441 tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc acactgatga
    19501 caggctactg ccgtggtcca gctgtggtct ttaatgacac gaatacggga aataaactcg
    19561 tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca agccacttca
    19621 caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt catgatcact
    19681 cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg atccaacgaa
    19741 ggaaatgtta aagttttggc acttgtccaa agcgttatgt gatcgcgccc aggtcatccc
    19801 tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgcagt ggtttcagga
    19861 gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac acggcgtacg
    19921 gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg agttcgtttt
    19981 cttctctgtc catcgccgct ccgctttcac gaatgtgcgc cagttcgtcg aacatcgcgg
    20041 gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg ttttcccagt
    20101 atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt gcggaacagt
    20161 agagcggcat atgctggcca atataggcac gggttcgcag catcccggtt gtgggttcca
    20221 gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg gtttcaccag
    20281 tggcgatgtt cagtgcctca agatgcggag cggcgatatg aatgatattc agcgaagaca
    20341 gcgccttctg cccgacggca ataaatttgg tagtcaggcg gtaactccct gcggcgggcg
    20401 cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg gtactcttat
    20461 ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag ttgctcaaaa
    20521 tctcaatcag catcaacccg cgaaacagac tctggcttcc ggctggacgc tctttttcct
    20581 gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg cgtcctgatg
    20641 gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc acttgatttt
    20701 tatgtctttt gaaattcatc aatcagattg ctttgttaaa aagtgatcga tatatttgaa
    20761 atcaagtttc gcatattgaa attttaagcc aaaaaagcga tcaaaaaaac aaggaagcct
    20821 gggatgaaag tgacatttga gcagttaaaa gcagccttta atcgggtctt aatttcacgc
    20881 ggcgttgaca gcgaaacggc tgacgcctgt gcagagatgt tcgcccgcac caccgaatcc
    20941 ggcgtttatt ctcacggcgt taatcgtttc cctcgtttca ttcaacaact ggaaaacggc
    21001 gatatcattc ctgatgccca acccaaacgt ataaccagcc tcggcgcaat tgaacagtgg
    21061 gatgcccagc gttcgatcgg caacctgacg gcgaaaaaga tgatggatcg cgccattgaa
    21121 ctggctgccg atcacggtat tggtctggtg gcacttcgta atgccaacca ctggatgcgc
    21181 ggcggcagct acggctggca ggcggcggaa aaaggctata ttggcatttg ctggaccaac
    21241 tccatcgccg taatgccgcc gtggggcgca aaagagtgtc gcattggcac taacccgctg
    21301 atcgtcgcca ttccttccac gccgatcacc atggtcgata tgtcgatgtc gatgttctct
    21361 tacggcatgt tagaagttaa ccgcctggcg ggccgtcagc tcccggtcga tggtggcttt
    21421 gatgatgagg gcaatttgac caaagaacct ggcgttatcg agaagaatcg ccgcattttg
    21481 ccgatgggct actggaaagg ttctggcatg tcgattgtgc tggatatgat cgctactctc
    21541 ctttccgacg gcgcatcggt tgccgaagtc acccaggaca acagcgacga atacggcatt
    21601 tcacaaattt ttattgccat tgaagtggac aagcttatcg acggtcccac ccgcgatgcc
    21661 aagctgcaac gtatcatgga ttacgttact accgctgaac gcgctgacga aaaccaggcc
    21721 atccgcttac ccggccacga atttactacc ctgctggccg aaaaccgccg taacggtatt
    21781 accgttgatg acagcgtgtg ggccaaaatc caggcgttat aaggagataa gtcatgattt
    21841 ttggacatat cgcgcagccc aatccgtgcc gtttgcccgc cgccattgaa aaggcgctcg
    21901 attttctgcg cgctaccgat ttcaacgccc tggagccggg cgttgtcgaa atcgacggca
    21961 aaaatattta tgcgcaaatt atcgatttaa ccacacgcga agcggtggaa aaccgtccgg
    22021 aagtgcatcg tcggtatatc gatattcagt ttctggcgtg gggtgaagag aaaatcggca
    22081 ttgctattga tacgggaaat aataaagtca gcgaatcact attagagcag cgcgatatta
    22141 ttttttatca tgacagtgaa catgaatcat ttatagaaat gataccgggc agctacgcaa
    22201 ttttctttcc gcaggatgtt caccgaccag gttgtatttt gcaaacagcc tctgagatac
    22261 gaaaaattgt ggttaaagtc gcattaacgg cgctgaatta atcgaataat cgtctacgcc
    22321 agaacgactg ggtaatgtta ttgctctaac tatattaata atttaggttg ctgcacatgc
    22381 aacttgaatt atgacagaca tagcctcaag gaatagctat gaaaaaaata cttgaagcaa
    22441 tactggcgat taatctcgcc gtactttcct gtattgtgtt tataaacatt attttaagat
    22501 atggttttca gacaagcatt ttatctgtag atgaattgtc acgttattta tttgtctggt
    22561 taacatttat tggcgcgatt gtggctttta tggataacgc ccacgttcag gtcacctttc
    22621 tggtggagaa actctccccc gcatggcaac gacgagtcgc tctggttacc cattccttaa
    22681 tcttatttat ttgtggcgca ctggcctagg gcgcaacgct aaaaacaatt caggactgga
    22741 gcgattattc accgatcctc ggtttaccca tcggcctgat gtatgccgct tgtctgccca
    22801 ccagcctcgt tatcgcattc tttgaactgc gtcatttata tcaactcatc acgcgtagca
    22861 attctctaac gtcgccaccg caaggagctt aatcatggct gtgctgattt ttctgggctg
    22921 tctgttgggt ggtattgcta tcggtttgcc tattgcctgg gcactgttgt tgtgcggggc
    22981 ggcgttaatg ttctggctgg acatgtttga tgtccagatc atggcacaaa cgctggtgaa
    23041 cggagccgat agcttctccc tgctggcgat tccgttcttt gtgctggcgg gtgaaatcat
    23101 gaatgcgggc ggcttgtcaa aacgcattgt tgacctgccg atgaaactgg tggggcataa
    23161 accaggcggg ctgggctacg tcggcgtgct ggcagctatg attatggcga gcctttctgg
    23221 ttctgccgtt gcggataccg ccgccgttgc cgctctactg gtgccgatga tgcgcagcgc
    23281 caactacccg gtcaaccggg cggcggggct gattgcctct ggcggcatta tcgcgccaat
    23341 tattccccct tccattccgt ttattatctt cggcgtttcc agcggattat ccatcagtaa
    23401 gctgtttatg gcgggcattg cccctggcat gatgatgggc gcaacgctga tgcttacctg
    23461 gtggtggcag gccagtcgcc ttaatttacc ccgccagcaa aaagcaacga tgcaggaaat
    23521 ctggcactct tttgtctccg gtatctgggc gctgtttctt ccagtcatca ttattggcgg
    23581 gtttcgctcc ggtctgttta cccctaccga agccggggcc gttgctgctt tctatgcgct
    23641 gtttgtcgcc acagttattt accgtgaaat gaccttcgcc acactttggc atgtactcat
    23701 tggcgcggcg aaaaccacct cagtggttat gtttctggtg gcctcggcac aagtttccgc
    23761 ctggctgatt accattgctg aactgccgat gatggtttct gatttactgc aaccgttggt
    23821 cgattcaccg cgtctgctgt ttatcgtcat tatggtggct atcctgattg tcggcatggt
    23881 catggattta acgccgacgg tattaattct taccccggta ttaatgcctt tagttaaaga
    23941 ggcaggaatc gatccgattt atttcggtgt catgtttatc attaactgct caatcggctt
    24001 aattacaccg cctatcggta atgtacttaa cgttatttca ggggtggcaa aactcaaatt
    24061 cgatgatgca gtcagaggcg ttttccctta cgtcctggtt ttatattcat tattagtcgt
    24121 atttgttttt attcccgatc tcatcatcct ccctttgaaa tggattaatt aaaaggaaaa
    24181 tattatgaaa ttacgctctg taacctacgc attattcatt gctggcctgg ctgcattcag
    24241 cacatcttct ctggcggcgc aatctttacg tttcggttat gaaacctcac aaaccgactc
    24301 gcaacatatt gcggcgaaaa aattcaatga tttattgcag gagagaacca aaggcgagct
    24361 gaaattaaaa ttgtttccgg atagcaccct cggtaacgcg caggcgatga tcagcggcgt
    24421 acgtggcggc accatcgata tggaaatgtc cggctcgaat aactttgccg gattatcacc
    24481 agtgatgaac ttgcttgatg tccctttcct gttccgcgat accgctcacg cgcataaaac
    24541 gctcgacggc aaagtcggtg atgatctgaa agcctcactt gaaggtaaag gactaaaagt
    24601 actggcctac tgggaaaacg gctggcgcga tgtcaccaac tcgcgcgcac cggttaaaac
    24661 ccccgccgac ctgaaagggc tgaaaattcg caccaacaat agcccgatga atatcgccgc
    24721 attcaaagtc tttggcgcta acccgatccc gatgccgttt gccgaagtct ataccgggct
    24781 ggaaacccgc actatcgacg ctcaggaaca cccgatcaac gtcgtctggt cagcaaaatt
    24841 tttcgaagtg cagaagtacc tttctctgac gcaccacgcc tattccccgc ttctggtggt
    24901 gatcaacaaa gcgaagtttg atggcttaag cccggagttc cagcaagcgc taatttcatc
    24961 tgcgcaggaa gcgggtaact accagcgcaa actggttgcc gaagatcagc aaaaaatcat
    25021 cgacggcatg aaagaagcgg gcgtggaagt catcaccgat ctcgaccgca aagcctttag
    25081 cgacgcactg ggtactcagg tccgcgacat gtttgtaaaa gatgttccgc agggtgctga
    25141 tctactgaaa gccgtggatg aggtgcaata aaccatgacg caatactggc tggggttaga
    25201 ttgtggcggt agctggctga aagccgggct gtatgaccgc gaaggccggg aggcaggcgt
    25261 gcagcgcctg ccgctgtgcg cattaagccc gcagccaggt tgggcagagc gcgatatggc
    25321 agaactgtgg caatgctgca tggctgtcat tcgcgaactg cttacccatt ccggcgttag
    25381 cggggaacaa attgtcggta tcggtatctc cgcacaagga aaaggcttgt ttttgctgga
    25441 taaaaacaac aagccgctcg gtaacgccat tttatcctcg gatcgccggg cgatggaaat
    25501 cgttcgtcgc tggcaggaag atggcatccc ggaaaaactc tacccgctga cccgacaaac
    25561 cttgtggacc gggcatccgg tatcgctgtt acgttggctt aaagagcacg aaccggaacg
    25621 ctacgcgcaa attggctgcg tgatgatgac gcacgactac ctgcgctggt gtttaaccgg
    25681 cgtcaaaggc tgtgaagaga gcaatatttc cgagtccaat ctctacaaca tgagtcttgg
    25741 ggaatatgac ccgtgcctca ccgactggct ggggattgct gaaatcaatc acaccctgcc
    25801 gcctgttgtc ggatctgccg aaatctgcgg ggagatcacc gctcagacag ccgtactgac
    25861 cggtctgaaa gcgggtacgc ccgttgtcgg cggcctgttt gatgtggttt ccaccgcact
    25921 ctgcgccggg atcgaagacg aatttaccct taatgcggtg atgggcacct gggcggtgac
    25981 cagcggcata acccgcggtt tacgtgacgg tgaagcgcat ccgtatgtct atggtcgcta
    26041 cgttaacgat ggtgaattta tcgttcacga agccagcccc acctcttccg gcaacctcga
    26101 atggtttacc gcacagtggg gagaaatctc gtttgatgag atcaaccagg ccgttgccag
    26161 cttgccgaag gccggtggcg atctcttttt cctgccgttc ctgtacggca gcaacgccgg
    26221 actcgagatg accagcggtt tctacgggat gcaggccatt cacacccgcg cacacctgtt
    26281 gcaggccatt tatgaagggg tggtgttcag ccatatgacc catctcaacc ggatgcgcga
    26341 acgttttact gatgtgcaca ccctgcgcgt cactggcggc ccggcgcatt ccgatgtctg
    26401 gatgcaaatg ctggcggacg tcagcggtct gcgtattgag ctgccgcagg tggaagaaac
    26461 cggctgcttt ggtgcagcac ttgccgcccg cgtcggcacc ggggtttatc gcgatttcag
    26521 cgaagcccag cgtgatttac agcacccggt gcgcaccctg ctgccggata tgactgtcca
    26581 tcagctttac caacaaaaat accaacgcta tcagcatctc attgccgcac ttcagggctt
    26641 tcacgcccgc atcaaggagc acatattatg agccgaccac ttctgcaact ggcactcgac
    26701 cactcatcac ttgaagccgc acagcgcgac gtgacgcagt taaaagacag cgtcgatatt
    26761 gtcgaagcgg gcaccattct ctgtttaaac gaagggcttg gcgcggtgaa agccttgcgc
    26821 gaacagtgcc ctgacaaaat catcgttgcc gactggaagg tcgccgacgc tggtgaaacg
    26881 ctcgcgcaac aggcatttgg cgcaggcgct aactggatga ccatcatctg cgccgcaccg
    26941 ctcgcgacgg tggaaaaagg ccacgcgatg gcgcaacgct gcggtggtga aattcagatc
    27001 gagctgttcg gtaactggac gctggacgac gcccgcgact ggcatcgtat tggcgtgcgg
    27061 caggcgattt atcatcgcgg tcgtgatgca caggccagcg ggcaacagtg gggcgaagcc
    27121 gatctggcac gcatgaaggc gctttccgat atcggccttg agctttccat taccggcggg
    27181 attacccctg ctgacctgcc gctgtttaaa gatattcgcg tgaaagcgtt tattgccggg
    27241 cgagcactgg caggctcagc aaatccggcg caggtggctg gcgatttcca tgcgcaaatc
    27301 gacgctatct ggggaggcaa gcgtgcgtaa tcatccgtta gggatttatg aaaaggcgct
    27361 ggcgaaagat ctctcctggc cggagcggct ggtgctggca aaaagctgcg gttttgattt
    27421 tgtcgaaatg tcggtggatg aaaccgacga acggctttca cgtcttgact ggagcaccgc
    27481 acaaaggact tcgctggttg ccgcgatgat cgaaacgggt gttgggatcc cgtcgatgtg
    27541 cctgtccgca catcgtcgct ttccttttgg tagccgtgac gacgcggtac gccagcgagc
    27601 acgggagatc atgagcaaag cgattcgtct ggcacgcgat ctcggcatac gcaccatcca
    27661 gctggcgggc tacgacgtct attacgaaga ccacgatgaa ggcacccggc aacgttttgc
    27721 tgaagggctg gcgtgggcgg tcgaacaggc ggcggcatca caagtcatgc tagcggtgga
    27781 gattatggac accgcgttta tgaactccat cagtaaatgg aaaaagtggg acgagatgct
    27841 cgcctcgccg tggttcaccg tctacccgga cgtcggcaac ctcagcgcct ggggcaatga
    27901 tgttcccgcc gaactgaaac tgggcattga ccgtatcgcc gcgatccacc tgaaagacac
    27961 ccagccagtt accgagcaaa gtcccggaca gttccgcgac gtgccgtttg gcgaaggctg
    28021 cgtcgatttc gttggcatct tcaaaacgct gcataaactg aactatcgcg gttctttcct
    28081 gattgagatg tggaccgaaa aagccaaaga gccggtgctg gagattattc aggcgcggcg
    28141 ttggattgaa gcgcgtatgc aggaggctgg atttatatgt tagagcaact gaaagccgac
    28201 gtgctggcgg cgaatctggc gcttcccgct caccatctgg tgacgttcac ctggggcaat
    28261 gtcagcgcgg tagacgaaac gcggcaatgg atggtaatca aaccttccgg cgtcgagtat
    28321 gacgtgatga ccgccgacga tatggtggtg gttgagatag ccagcggcaa ggtggtggaa
    28381 ggtagcaaaa agccctcgtc cgacacgcca acgcatctgg cgctttaccg tcgctacgcc
    28441 gaaatcggtg gcattgtgca tacccactca cgccacgcca ccatctggtc gcaggccggg
    28501 ctggatctcc ccgcctgggg caccacgcac gctgattatt tctatggtgc catcccctgc
    28561 acgcgacaga tgaccgcaga ggagattaac ggcgaatatg aatatcagac cggcgaagtg
    28621 atcattgaaa ccttcgaaga acgtggcagg agtccggcac aaatcccggc ggtgctggtg
    28681 cattctcacg gcccgttcgc atggggtaaa aacgccgccg atgccgtgca taacgccgta
    28741 gtactcgaag aatgcgccta tatgggccta ttctcgcgcc agcttgcgcc gcagctccct
    28801 gcgatgcaaa acgaactgct ggataagcac tacctgcgta agcatggggc caatgcctat
    28861 tacgggcagt aatccctcac gccggggctt catcgccccg gcactacgaa ttgatatgtt
    28921 ccttgctgta acgccgcttc cacgctgctg gcgttaaacc ggtgtgcttg cgaaaaatct
    28981 gccggaaata gccaacgtca ttaaaccgta ttgcatggag caggccgaca acgtcgagtc
    29041 actgcataac ctgttgctgc ccgccgatgc gccatttacg acatttgaag gcaagggatt
    29101 gttcagccat aagatcctga ttcagacgcc aggttcccgg tatgaacaga gcaatccaga
    29161 gcaacaacct ttgtggcatt acgacggagc accagccgca ccgacatcca ccggtgaatt
    29221 aaaaacgcca gctcacgccg gtgataaagg taaaactctc attacgatca atcatcgggc
    29281 tgttggtaat ctcttccggc agctcgctgt atcctgcgct cgccatcaat acgacgtgct
    29341 ctcctatcgg gtattttgcc gtcaggctga catagggcac ccaggcatcc tgcgcggaat
    29401 aacttgccag accgctacga cgggactcac tctctgaaat gccatagtaa tagtcactga
    29461 aattctcgtc ataatagaga acgcccagcg caggtgtcag cgacaggcga cctatctgca
    29521 ttttgtggaa taccgatagc tcccccaccc agccgttgct gttatccaga acgtccgcag
    29581 ccgcagaggc tttcacggtt ccccaacgct cgtggtgata ccaggcaacc cccgccatag
    29641 ccgtactatc acgcttatca agctgttgca tggcataatc gtcattatcg gcagggtcaa
    29701 attccagcgg catccaggat gcggtcaggc taagttcatt tttttcactt tgcgacagaa
    29761 taaaacctaa tgtggtctga cgaacataaa aagaatcacc ttcataacta atcagcggaa
    29821 ttgcctttgt attttcatta taaccgcgat aaggcgattc attataagct gcaccagcac
    29881 caatggataa ttcagaagca gttgcgcttg ccataaaagg caacgcaaat aacgccacaa
    29941 tattgcgatt aattaacatg tccggtattc cattttaaaa taagataaaa aagagtcggc
    30001 ataataat
//
LOCUS       DAZFJF010000053        29613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFJF010000053 DAZFJF010000000
VERSION     DAZFJF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.053"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKAU_002529"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKAU_002529"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO5531575.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="ACHKAU_002530"
     CDS             complement(804..1352)
                     /locus_tag="ACHKAU_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO5531576.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1536..3383)
                     /gene="ldtD"
                     /locus_tag="ACHKAU_002531"
     CDS             complement(1536..3383)
                     /gene="ldtD"
                     /locus_tag="ACHKAU_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5531577.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3644..8104)
                     /gene="mukB"
                     /locus_tag="ACHKAU_002532"
     CDS             complement(3644..8104)
                     /gene="mukB"
                     /locus_tag="ACHKAU_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO5531578.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8104..8808)
                     /gene="mukE"
                     /locus_tag="ACHKAU_002533"
     CDS             complement(8104..8808)
                     /gene="mukE"
                     /locus_tag="ACHKAU_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO5531579.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8789..10111)
                     /gene="mukF"
                     /locus_tag="ACHKAU_002534"
     CDS             complement(8789..10111)
                     /gene="mukF"
                     /locus_tag="ACHKAU_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO5531580.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10108..10893)
                     /gene="cmoM"
                     /locus_tag="ACHKAU_002535"
     CDS             complement(10108..10893)
                     /gene="cmoM"
                     /locus_tag="ACHKAU_002535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO5531581.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11029..11808
                     /gene="elyC"
                     /locus_tag="ACHKAU_002536"
     CDS             11029..11808
                     /gene="elyC"
                     /locus_tag="ACHKAU_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO5531582.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11785..12678)
                     /locus_tag="ACHKAU_002537"
     CDS             complement(11785..12678)
                     /locus_tag="ACHKAU_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO5531583.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12832..13578)
                     /gene="kdsB"
                     /locus_tag="ACHKAU_002538"
     CDS             complement(12832..13578)
                     /gene="kdsB"
                     /locus_tag="ACHKAU_002538"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5531584.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13575..13757)
                     /gene="ycaR"
                     /locus_tag="ACHKAU_002539"
     CDS             complement(13575..13757)
                     /gene="ycaR"
                     /locus_tag="ACHKAU_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO5531585.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13809..15041)
                     /gene="ycaQ"
                     /locus_tag="ACHKAU_002540"
     CDS             complement(13809..15041)
                     /gene="ycaQ"
                     /locus_tag="ACHKAU_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO5531586.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15078..16064)
                     /gene="lpxK"
                     /locus_tag="ACHKAU_002541"
     CDS             complement(15078..16064)
                     /gene="lpxK"
                     /locus_tag="ACHKAU_002541"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO5531587.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16061..17809)
                     /gene="msbA"
                     /locus_tag="ACHKAU_002542"
     CDS             complement(16061..17809)
                     /gene="msbA"
                     /locus_tag="ACHKAU_002542"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO5531588.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17846..20110)
                     /locus_tag="ACHKAU_002543"
     CDS             complement(17846..20110)
                     /locus_tag="ACHKAU_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO5531589.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNRSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20317..20601)
                     /gene="ihfB"
                     /locus_tag="ACHKAU_002544"
     CDS             complement(20317..20601)
                     /gene="ihfB"
                     /locus_tag="ACHKAU_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO5531590.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20761..22434)
                     /gene="rpsA"
                     /locus_tag="ACHKAU_002545"
     CDS             complement(20761..22434)
                     /gene="rpsA"
                     /locus_tag="ACHKAU_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO5531591.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22545..23228)
                     /gene="cmk"
                     /locus_tag="ACHKAU_002546"
     CDS             complement(22545..23228)
                     /gene="cmk"
                     /locus_tag="ACHKAU_002546"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO5531592.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23401..24165)
                     /gene="ycaL"
                     /locus_tag="ACHKAU_002547"
     CDS             complement(23401..24165)
                     /gene="ycaL"
                     /locus_tag="ACHKAU_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO5531593.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24334..25617)
                     /gene="aroA"
                     /locus_tag="ACHKAU_002548"
     CDS             complement(24334..25617)
                     /gene="aroA"
                     /locus_tag="ACHKAU_002548"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO5531594.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25688..26776)
                     /gene="serC"
                     /locus_tag="ACHKAU_002549"
     CDS             complement(25688..26776)
                     /gene="serC"
                     /locus_tag="ACHKAU_002549"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO5531595.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26975..27667)
                     /locus_tag="ACHKAU_002550"
     CDS             complement(26975..27667)
                     /locus_tag="ACHKAU_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO5531596.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGKIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27797..29557
                     /gene="ycaO"
                     /locus_tag="ACHKAU_002551"
     CDS             27797..29557
                     /gene="ycaO"
                     /locus_tag="ACHKAU_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO5531597.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7190 a         8056 c         7191 g         7176 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgtc acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgatgact caaccccagc agtgacgggg gctgcagaga atcgccccca atcatgctaa
     1501 ttattacgac aactgatttc cccgaactac ttcatttacc tgattaattg ttccgctttc
     1561 gatacgattt gcgagctgga tcgcgcaggc agatcataat tgtaaatatc tgtacgatac
     1621 tgggtacgac catctgcacc aacaaaggcc gtcaggtagt agagattcac cggaatcgac
     1681 tgccgaatat tgacgtaacg tgtatcaccc tgcttcagcg catcagaaat acgtttgtca
     1741 ttccagcctg catcctgcaa cagcatattc gccagatcgg aagctttatt cactcgtaca
     1801 cagcctgagc tcaatgcgcg tgtatcacgc ttgaacagat tgtggttcgg cgtgtcatgc
     1861 aaataaatgg cctctgaact cggcatattg aatttatagc gccccagcga gttccgtggg
     1921 cctggagcct gctggaagcg gaacggtaaa ttcgaggccg tgattgtaga ccagtcaacc
     1981 tgccatgggt caatcgcttc tctgctgttc cagccgcgca tcaccgtata gccatggctt
     2041 tcgagatatc ccggatcgtt gcgcactttt ggcagaatat ctttgcgtgc cagagttgga
     2101 ggtacgttcc acggcgggtt taccactacg ttgttaaggg cactgctcat catcggcgtt
     2161 ttgcgatcgg ggcgaccgac aatgactcgc gaatccagca cctgattgcc gttctgatag
     2221 tagaccagcg aataggccgg aatgttaacc atgatcccgg tagaaagctc tgttggcagc
     2281 aagcgcaatc gctggatgtt gagagccaac acgccagcac gctgggcggg cgttacgttt
     2341 aaccagtcac gtgttgccgg gccaatagca ccatctgccc ccaatccttg ccatgcctga
     2401 aaacgtttaa cggcttccac cagttcatta tcgtaggcgg cacgaacggc aggcgcaggt
     2461 ttactacgag acgtcgtttg cttgtccatc ggcttagttt ctgctgtttc aacagtaaca
     2521 gcggatgggc tgactaccgc gtcagttggc gtgtcatcgc caggtagagt aattttcggc
     2581 cccccgtcca acatgcctgt gcgttgcaat atttcgcgca acgccggtac gtcgttactc
     2641 cactgccctg ggcgcaacgt tgctttgccg gtcagttggg gccacggttt ggtgtcactg
     2701 agtaaggcca gtaacgattc atgcatcgcc gcatattgcg gatgctgcgg tgccagtcct
     2761 gcaacaaacg taggcaattg acctttatcc agcgccagct gccattggtt aatcaccgag
     2821 agcggcggcg ttgcaagtgc ataaggttta ctgctatata gccagcgagt gcctttgacc
     2881 ggaatatttg caatgaaatg gagatagccc atcatcgcat cagagagcac tacgtcgcgt
     2941 gccatcccgt taacaccagg atcggtcagt aactctaccc atttattaaa ctgcggctgg
     3001 aaaccggcaa tcgccacctc tgccagctgt tgctggaagg ctttaacagc atcacggttt
     3061 tcccacatgg gttgcatatc gcgtgcggca tacaacagtt gaagctggtt aagataaacc
     3121 ggcttgtaac ctgcgggtaa ttgagattcg atttgcgtgc gggctttttc tgccgcacct
     3181 tcaggcaatg gctggatccc cgccattatt gccgtggctt gcgcctgcgg cagtgcctcg
     3241 ccctgttcac tgacagccac cgggctgtcg ccagggatta cttcaggctc atcggcctgc
     3301 gcattgaaca gtggagcgaa tgttacggcc aggcacaaac tgattgccga cagccgacga
     3361 ccacacatca tattaagcaa catcccttgc cccctgtttt tatcatactc tgaatagcca
     3421 ggctgttatt ttacgacttg cctgtacaac gccaatactc acgaaagtat gacgagcaag
     3481 cctgttactc atcaggctta tcttcagtat ataaacgccg atacgttttt acataaccta
     3541 atgtaaagaa gtttaaagaa aagcgcgccg taatgtgcca cttttttagt gcggaaacgg
     3601 agttttcgga aaaagaaaag gcggcattgc tgccgcctta attttaactc gcctgagaag
     3661 gcgcttcgtc acttcctgga agcgtttcag ggagttgcgg cgcaaatcct cgcaggccga
     3721 cgacgtgaac gtgttcggta ttctggaaga ctttacgcac cagtttatag gtggtgcctt
     3781 tctccgggct gatattttcc ggcgctgcga tgatgagctg catttgcaaa cgctcacaca
     3841 attcaaacag cgtggcgata gaacgagcat ccagtcgcgc tgcttcatcg aggaacagca
     3901 ggcggcaagg agagatatct ttaccgcgca ggcggcgaga ttcatcttcc cagctttgta
     3961 ccaccatcac cagaatcgac ataccggtac caatcgcctc accggtcgac aatgcaccag
     4021 actctgcgcg caaccaacca tcggaaccac ggttaacctc aacttccatt tccagatagt
     4081 tgcggtaatc cagcagttct tcgccaatgg tctgcggcgt gcgctgtccc atatcaatct
     4141 gcggattaag acgctgatac aatttcgcca acgcttccga gaaggtcaga cggttgctgt
     4201 taaacagatc ctgatgctgc tcgtgctgtt cagagagcac atccagcagc atggcgtgcg
     4261 tttcacgcac gttcacgttg aggcgcacgc tgttcacctg accaaacgat acgttctgca
     4321 acccctggtt gagcatacgg atacggttct gctcgcgctg aatggttttg cgaatgatgt
     4381 tcgccacgct gcgggaactg atcgccagtt tctgttcacg ggaggttaat tcttcggtca
     4441 gacggctaag ttcgatctcc atctgttcga tagcttccac cggatcatcg gtacgaataa
     4501 tatcctgacg aatacgttca cgcagatgct gataaaccgc cacgaagaac tgaattttac
     4561 gctccggacg tttcggatct tccgacatgc gcagcacgtc gcgcagatgt tcgttatccg
     4621 ccaccgccag acgcagcgca cccaatgcct tatccgacat agagcgcaaa tcatcagcgg
     4681 agagataagc cagctcacga cggtgtaagc ggcgctcaac gccgttatct ttcaccatgc
     4741 gcatcaccgc acaccagccc gctttggcgg tcactacctg ctcgcgcatc tcaaagtaat
     4801 cccgctccag cttgcgcagt ttgcgggtca ggttgtccat ctccgcttcg cagaaggtaa
     4861 gcgctttttc cagttgattg cggcgtgaac ggttattgct cagttgcgca tgcagctcgt
     4921 cacggcgaat acgcgcccgc tcttctgccc cgctatcagc acgcacgccg atatcctgca
     4981 attcacgttg cagatcgttg agtagctctt ttttggtgtc gtaagaactt ttcagcgaag
     5041 ccagcacctg gttgtactga ctcaactgcg ctgcgtgacc acgcaacgct tcacgagcgc
     5101 gagtacgctc cgcttccgcc tgttccagac gttcacgcag cttttcgttg agatcgctat
     5161 taccgctcag catttctgcc gagtcagaat agctaaagtg cgcacgacgc tgcaccactt
     5221 ccgtcagggc aaacgcctgc tgacgggcat cgcgctgcat ctgctgagag tacgcgtaat
     5281 cttcttttag ctgttcgaac tgttccgggt cgctctgcaa taccgaaacg atcggttcca
     5341 gtttcgccag ttgattgcca aactgctgaa caaaacgcgc ggcttcctgg gcttcatcca
     5401 gacgttcgcg gatttcatcg acgcgatccg ccaggctgtc atcagccaac aggttgagac
     5461 gcggcagaat gcggttcagc gccgtaacgc cttctttcgc ctgctcaaac tgaatacgct
     5521 gctgctggtt atcattttca tgattactca acgcccgctc cagttcgacg cgacggctgt
     5581 tcagttgacg gatttctgct tccgggtcag actcaaacgc aaccgccaga tgactgccga
     5641 taaagcggct gaacgcctga tgcagacgct gagttttctg tacatcaaag gagagcgtgg
     5701 cgaagcgttc ggaaagcact tcacgctcgg catggaggct ttcaatacgg ctttcacgcg
     5761 cagcacgacc aaacagcggc acttccggga aacgtgaata acgccactga cgatcggcga
     5821 ttttcaccac taccgctttt tccagctcat caacgctgaa cacgctgtca tcgaatgact
     5881 gcggatctcc ttcgatcaga tagagatctt ccgggcaatc ggtcaagcct tccaggtgtt
     5941 cagttatctg tgacagatct ggcaccacga tggcgtggcg tgacgggcca tacagcgctg
     6001 agaagtacgg cgcatcttcc aggctaacgt cgtcataaat ttctgacagc agcacaccac
     6061 caaaacgctc cgccagcgcg ttcagacgct gatcttcaga gccgccaggc tggcttaaac
     6121 gttcgatctc ttcatcgacg gcgtttttgc gcgcgcccac ttcatcgcgt tcaacaatcg
     6181 cctctcgctc acgctccagc aactgttgca gatattcggt gacgtcctgg ctggaggtaa
     6241 actcttcgcc gcactgttcg ctcaactggt tgagactgtt ttgcgctgcc agccaaaccg
     6301 gcgcacgctg catcaaactc tgaatgcgag actgcagctg ttcctgttcc tggcgcagtg
     6361 ccatgcgctc ttcacgggcg ttagacacgc tatcggaaag agaggcaatg cgcgcttcca
     6421 gctcctgatg cagagcttcc agctcgtcaa tgtcaaaatt cttgccctga cgtttgcaga
     6481 aatctgccag cagacgctca gcttcttgct gttcgcgcag acgctgttcc agttcgctta
     6541 agcgcatccg caacggctga acctgctctg ccaggtgacg ctgatcgacc ccttcgcgca
     6601 atagttcgcg agcgacatcc cacgcctcgt tacgcgccag cgggccgttg attgccacca
     6661 ccagctgata agcctgctca aactggctgt gcgcggtttg cgccatgctc attttctgct
     6721 caagagagag cattttttca gtcgcttcca gctctttcgc ctggaaggtt tccagccatt
     6781 cggcggcgct gtcggcggtt aagtccggca gatggcacag ttctttggcg cgattaagcg
     6841 cagcaatcgc ctggttatac tggatcgcgc gcgtttgctg gacgtccagc gcctgctggt
     6901 agttagcaag ctggcttttc agctcgtcca cttccagttc ggcagcttcc gcgcgagcct
     6961 cgttctcttc ctggcgttcg atggcttctg ccaccacttc gttttgctct tccagacgga
     7021 tctgcagctc atcgagatcc gcttcgtagc gttcgatctt ctcctgctga cgcagtgcgg
     7081 tttgcaccag gttcaggtga tcgctggccg cctgataatc cgcttccaga tcgccttcgg
     7141 caccgttgtg ctctgccagc tcacgcgcca tatcgacgtg tttgtactgc tcagccgcca
     7201 gttgctgacg cgaagtatgt aactcgcgac gaaactccag ggctttgtcg agatggacac
     7261 ggcgctcgtt ggcgtgacgc atgtagtccg ccgccacgta gttggtagct tcgctgatca
     7321 gatgcttaaa caggtcacgg tccgactggg tgacacgaat cgcttccagc gtcatgcggt
     7381 tttcacgcag cgccgcttcc atgtcctgga acgctttacg cacgccgctg ttttccggca
     7441 acaggtagtc gcgcagagaa cgggtaatgg cactggagat cccgccatac agcgaagctt
     7501 cgatcagacg atagaatttg ctacggtcag atgcggagcg cagacgacgc gcgatgatgc
     7561 ccagatcgaa catcagcgag tggtaatcgg taatggagtt gaactgttta aactgcacgc
     7621 cttccatcgc ctcgagcttg tctttcagct cgttaagcgg cagcacgcgc gcctggcgtt
     7681 cgttcagggt ttcggtcacc agctgtgtcg gctgcaccga catcggcagt ccctgaatgg
     7741 caaatggttt gatatccact ttacgatcgc gtccggcaac ctgttgcaga cgcacaccga
     7801 ccaccacgcg ctggtggcgc gagttaatgg tgtcgagcat cgaataacag acacccgctt
     7861 tcagcttacc gtgcagacct ttatcgcgcg aaccgctggt ggccccggct tccgtagtgt
     7921 tacggaaatg cagcagggtc aggtcgggga tcagcgccgt aacgaacgcc gccatggtgg
     7981 tggatttacc cgccccgtta ccgccagaaa gcgtcgtgac cagctcgtca aggtcaaaag
     8041 ttcgggcaaa aaagccgttc cagttaatca gcgtcagtga gcgaaattta ccgcgttcaa
     8101 tcattattct tcctctccgc tatctggctg attttcttcg gtttcatcgt tgagttgcag
     8161 atgattttca atcggcattg cttcgccatc acgaatcagg cgacgctgtg cttcacgggg
     8221 atcgtcgcca gcacgcacat cggctccgaa gcggaacacc gattcggtaa tgcgaaactt
     8281 gctgctgtcg tggcccataa accacaccat gcctaaacga cgcagacggt tgagcgaaga
     8341 acgtactttc tcctgcaact tctgacggtc aacgtctgaa ccggttgaac ggttgttcac
     8401 cagtttcagc agttttgctt catcggccag ggtgagcagt tcgtcgtaca gttcctgctg
     8461 ggtgaaaatc ccctcattcg ccagccgttc cgggctgaga tagagataac agaggatttt
     8521 cccgaccatc atatccagtt ccgacaagac ggaacgaggg atcagcgtgg tggaacgtgg
     8581 gcgtaaatag aagaaccctt ctggtgcgcg aataagctca acgttataac gcgcgtaaaa
     8641 ctcttccaga tattcctgaa aatccatcag gaatgcatga ttatccagtt cgtcgaggcc
     8701 aatatggcgg cctgaacgta aggcgctgtc cagcgccgga aataacggat tcgccagcgc
     8761 ctgcgccagc ttaaccggca tcacttgttc aatatttgtc gatgacatgc gcctgtacct
     8821 tggctccgta atcattaatc ggctgccatt tcgctggcag tccggtgaaa tctgcttgcg
     8881 ctacgccaag acgtaccgcc tgatcaataa caatacgcgc aacgtcaaag tgacgtgcac
     8941 gcggatactg tgacagatat tcgcgtacca ccagaccaag atccagcggc acttgtctgg
     9001 ttttgtacac ggcaagttgt tcttcgatga tcgccgccag ctgttcgcgg atctcgttaa
     9061 actcttcgta ttccagatcc tcaggaagtt ccccagtcac ttcttcatcg cgcagtgcca
     9121 tctcttcgtc acgcatatcc agcagacgat cggcattggc ataagttagc gcccacggct
     9181 catcaaaata ggtttgtacc gactgacgta accgctgagc aaagacgcgg tttttatcca
     9241 tatcgatcgc ggtacgaata aatttgtgta cgtggcggtc gtagccaatc cacaagtcga
     9301 tggattgctg gccccaactg ataatacgat cgagtttgct ctgcagatcg aacaccagac
     9361 gatcgacgaa atgcagatcg tcatgggtca tcgtcgcatc ctgaatgcgc aacagattag
     9421 cctgcaattt gtcgcctgcc gcttccagcg tatcctgcaa ttcacgcagc gtcccggaag
     9481 tttccgaaag caacaattca cagctggaaa tcgccgcccg ccagtctttg ttcagcaact
     9541 gggcgatatc gtccttcacc tgctgctgct gttcgtccat cagacgttgc gtcaggtcga
     9601 tactgtcgaa aatttctgct accgaatatt tcagtggcgc atagacatta cggtgccagt
     9661 gaaattcatc accgccctct tcggcggcat ctgctgcgcg tttgagctca cccgccacaa
     9721 tcgacaactg catagaaaga cgcagcgtag aaaactcgcg ctgacggatg tagtagtcag
     9781 taatgccgat gccgagcggc gtcagacggt aaattgcgtt cccttccgcc tgctcgctgg
     9841 taaagcggtt cagcagacgt tgacgcacca tgtcgttgat cgcgttattg gcgcgcacgc
     9901 cgatggtttc gctggtttgc tcaaacgcat cactcacatg gcggaatgca tccaccagct
     9961 cgccttcact catctcacca tccagacgct cgccgttcag cgtggcaacc gccagcagaa
    10021 aagagagtcg gtctaccggc agcgagatgg agaagtcatt ttttctggcc caggcaacca
    10081 gttcggggac tgtctgggaa aattcactca tactttatcc ttgctctgcg gtttgcgcgc
    10141 ggtgacatga atataacgcc ccagggtaat atacggttcc tgacggcaat aacgcgtttc
    10201 taattcaagt aatgcttcat agcagtcgcg ctgctggtgc ttctcgcgca gataatcatg
    10261 aaacacgcga acgcctgtct tacccataat ttgccaacca gcttcttcca gccacagata
    10321 aacctgcgcc gggtcgcgtg gataatctgg cgaaagcgtc cgttttttct ttttcggcat
    10381 tcccgcctgc acgtaatcaa aattcccggc gaccatgtta tgcatcaaca aaccatgcgc
    10441 attgtagaac attaacgaca acacgccgcc tggacgcaac actgaccaca gggtctgcaa
    10501 tacactgcgg ggatcggcca cccactcgag caccgcatgg aacaatatca gatcaacggg
    10561 cgtttccaaa tgcgaagcaa catcctgagc ggcgcaatgt ataaattgca tgttgtcgct
    10621 cacacctttt gcttctgccg cctgttttgc gcggtcgatc atctgcgcag aaagatcgca
    10681 taaaatgacc tgatgcccac gctcggccat tttgattgcg gtctgccctt ctccaccgcc
    10741 agcatccagc acacgcagtt tttgcgggcc catttccgcc agcacgcgat cgagatcctg
    10801 ccacagaata gcctgtcgaa gctgcccttt ggtggtgccg taaatgttac gggaaaactt
    10861 ttccgcaata tcatcaaaat tgcgatcctg cattgctttc tccaccggtt ggctaagacg
    10921 ttttctgata aagacgctat tttgtcacag ccacggcgag aatgaacccg tctggtgtaa
    10981 tatcgctggc gatcccttgc tatatggtta aaaaaggaac cagaaaggat gctttttaca
    11041 ctgaaaaaag tgattggcaa tatgttgctg ccacttccgc tgatgttgtt aattattggc
    11101 gctggcctgg cgctgctgtg gtttagccgt tttcaaaaaa ccgggaagat ttttattagc
    11161 attgggtggc tggcactctt gttattgagt ttgcaaccgg tagccgaccg cctgctgcgt
    11221 cccatcgaaa gcacctatcc gacatggaat aattcgcaaa aagtggacta tatcgtggtg
    11281 ctcggagatg gttatacctg gaacccgcag tgggcaccga gctctaattt aatcaataac
    11341 agtctgccac gcctgaatga aggtattcgc ttatggcgcg aaaatccggg atcaaaactg
    11401 atcttcacgg gaggcgtagc aaaaaccaat acggtgagta cagcggaagt aggtgccaga
    11461 gttgcgcaat cgctgggcgt gccgcgcgag caaattatca ccctggattt gccaaaagat
    11521 accgaagaag aagctgcggc agtgaaacag gcgattggtg acgccccttt cctgctggtg
    11581 acctccgcat cacatctgcc gcgggcgatg atcttttttc agcaggaagg gttaaatccg
    11641 ctaccagccc cggcgaatca gttggccatc gactcgccgc ttaacccgtg ggaacgggca
    11701 atcccttctc cggtctggct gatgcatagc gatcgtgttg gctatgaaac gcttggccgc
    11761 atctggcagt ggctgaaagg tccgtcaggc gagccacggc aagagtgatt ttgatgccag
    11821 atcgaagttg cgccgactaa atcgtccggt attaaccagt tgcgcaactt catcccataa
    11881 cacatacaac caacgccgcc agatgaacga ctccgccact ggcgcgcgtt gcaggtaact
    11941 ccagagcaaa tcttccgcca gagaattatc cattagtcgg aacagttcgt attctcgcgg
    12001 tgcccaaagc attagtcccg gcccgaccat cgccagtaac tgatcgctgc gcgaatcttt
    12061 cagcatgctg cgtaaacaga agttaccgtg aatcaacaca caattgtcgt taaagccttc
    12121 aaataatgcc gggagacatt cgcgagtgcg aaacaggatc cgcttatcct gcatcgtcag
    12181 accggtgtta ttgaactgat tgagcgtggt ccatagcact tcaacatgtt gcaggtacca
    12241 tgagggccag aaattttcct gagtattgtc gaccgcgccg acgcaaccgc gactgtcctg
    12301 acggtgccag gccagtaagg cttcaacgat ttggtctttg agctgttccc agcgttctgg
    12361 tgttcgggct ggtgcctcca ccgaaacacc acgcatccgc tccagtagca ggacatcagg
    12421 gccggggtgt tcttcatgcg tcatcacacc ataaatagtc ggcatacgga cagtcccact
    12481 tcttgccagc atggtggttt tccatgccag ttgtcgggct tttccgggcg tactaaagct
    12541 tcttgccatt aacggcattg ggtttccctg gctgtcatac aaagcccaca acgccgtatc
    12601 cgctttttca ttgacgcact caatacgact gagtttttcg cccagtaaat ggcttaattc
    12661 ggcacgcagc tgttccattc agattaccct cataaaagct actgctttca taatgagcgc
    12721 caatataaaa gatgtcacct gaatgagatc aattgttgaa aagaaaaatt gaggaaaatc
    12781 gtaacggtac gacactcctc ccaaaattgg ctgaagtgtc gtgaagtgaa attagcgcat
    12841 ttcagcgcga acgcgctcaa gatcttcagg ggtatccaca cctgtgccag gaacttcctg
    12901 agcaacagca acatggattt tttcgccgta ccacagaaca cgaagctgct ctaacatttc
    12961 gatgtgttct aacggacttg cctgccagtt gacgtaacga cggataaagc ctgcacggta
    13021 gccataaata ccaagatgac gcaggaagtt atcgccaacg gtttcaaggc cttctgcaaa
    13081 acgatcacga tcccaaggaa tggtggcgcg tgagaagtac agtgcatacc cttcagcgtc
    13141 gagaaccact ttcaccgcat tcgggttaaa cgcttcttcc gcattgtgga ttggcaccgc
    13201 cagagtcgcc atacccacct gacgctgagc gaggttatca gcaacctgac gaatgattgt
    13261 cgcagggatc atcggttcat caccctgcac attaacgatc accgtgtcgt cgctgaatgc
    13321 gcatttttcg acaacttccg ccagacgttc tgttcctgac tgatgatcgg cgcgcgtcat
    13381 acatacttca ccgccagcgg cttcaacggc gcgggcaaca tcctcatgat cggttgccac
    13441 gatgatgcgc tcggcacctg attcacgcgc gcgttcaaga acatgaacaa tcatgggttt
    13501 gccgttaata tcaaccaatg gtttaccggg cagacgcgtg gacgcgtagc gcgcgggaat
    13561 aatgaccaca aaactcatga tttactctca tcagcagtca gcacgcgggc ttccgtttcc
    13621 agcaacactg gaatgccatc gcggagcggg aaagccaggt tatccagttt gcaaataagc
    13681 tcttgttttt cctggttata ccaaagtttt ccgttgcaaa ccgggcaggc aatgatttca
    13741 agcaaacgat gatccatagt tcctccagat ggatcgggtt atgaatgcat aaatcttatc
    13801 ataatcattt atgcgacggg gtctatttcc cagccagtgc ggcaatcagt aaagagacct
    13861 tgcgggcagc gtcctaatgt cacccgcgtt gcctgctgcc agctcgcgaa atcagtaatc
    13921 gcctgacgta accctttttg cagcattgtc gttggtttaa tgccttcctg taaccacaga
    13981 gagataactt caaggatgcc tgtctggcga tgcattttgg catccattcg cccaactaat
    14041 tgcccacgat gtaataatgg cagaacaaaa tagccatact ggcgtttcgg cgctggggta
    14101 tagcactcca gccggtagct aaaatcaaaa agctgctctg cgcgtttgcg atcccagaca
    14161 acaggatcaa aaggcgaaag taccgcgctg tgcgttgcag tgagctttcc ggccagcgca
    14221 cgctcgagta gcggcagcaa atcagcatgc agccaaagat tgcccaattt ttcaacatgc
    14281 acagcaatga tttgctgctg ttcagccctc gcttcgcgcc acgccgccag tgccggacgt
    14341 ttcagccgat agtaatctgc cagccactgt tcgcggaata ttcccaggct acgcgcactg
    14401 ttatccagca tgatgatttc tgcttctgtt tgcgaaacga gatcgcgctc atcatcccag
    14461 tcaggcatga cacggtgggt taaatcataa acgcgctgga agttgcgccg ttcaatcacc
    14521 atcacctttc cggcagtaaa taaaccttcc agatgccgtt tatgcggctt ccattcccac
    14581 cagccgcttg cacctttacg aggatgctca aaatcggctg aacgtaccgg ccccttatca
    14641 tgaatatgct gaattaactg tgcaatttcc gcctcatgtt cctgcatcca ggcgtctttg
    14701 tatttccagc ccattttttc aggtgccagc atgcggtggc gaataagacg aaagtcgcta
    14761 cgcggcataa agcaggcttc atgcgcccag tattccatta attcgccacg cgccagagac
    14821 tcatccagcc actgggcagg ataatttccc agacgactga aaagcaccag atatggacta
    14881 cgggcaacaa tattgatggt atcgatttgc agcaaggaca tgcgggagat cgttgccgga
    14941 atatcctcca acgacgcttg acggcggggt ttgtttaaca ggccttgtgc ggcaaggtga
    15001 agattacgcg catcagcaag ggagaggtgc ggcagcgaca ttcatgactc catcaatcga
    15061 acgctgccgc ggcgtaacta gttgccagaa gccagcgagg ttagttgcgt aagcagtttc
    15121 gctggttcat cacctgaaag ctgtgcgtct acaggcaaat accaccaatt ttcttctgca
    15181 aaggcccggc atttcaccgc atctttttca gtcattacca gcgtttgccc ggcgcttacc
    15241 aacgcactga catccgcatg gttcaaagac tgatgatcgg ccagcggtac acatttttcc
    15301 ggttgtacgc cacacatctt cagcgtggca aaaaagcgcg gcggatgccc aatccccgcc
    15361 atcgccacta catgttcaag ctgagcaacg tcacaacgcg taccggtacg taaattcacc
    15421 gcctgacccg gcagcagatg catggggatt tcaccgctgc gagggacacc gccgttgacg
    15481 attaccgcat caaccgactt taagcgcccc gctcgctcac gcattggccc cgccggcaac
    15541 caccagccat tgccaaagcg acgcacacca tcaataacga caatttccac atcacgcgcc
    15601 agacggtaat gctgtaaacc gtcgtcggtt acgatgatct gcacatcagg gtgttgcgcc
    15661 agaatggctt ttaccgcatc agaacgaacg ggagaaaccg caacaggcgc atcagtgcgt
    15721 tgataaatca acacaggttc atcacccgcc tgtgctgttg tggtatctgc cgacaataac
    15781 agcggataag attcagcctt accaccatat ccccgcgata cgacccccac gcgaataccg
    15841 cgctgttgca actgttccac cagccagaca acgaccgggg tttttccgtt gccgcctgcg
    15901 gtgagattac caaccacgac aaccggtacg ggggcacgcc aggcgcgctt cagttttagt
    15961 ttatagcaaa gacggatcgc gccacttacc aggccataca accaggagag tggcagcaat
    16021 agccgccaca aaggggattc accagaccag attttttcga tcattggcca aactgcattt
    16081 tgtgaagttg cgcgtaaacg ccgcggtgct caagcaaatc gttatgcgta ccgcgttcca
    16141 caatgacacc atcctcgacg accacgattt cgtctgcctt ttcaatggta gacaagcggt
    16201 gggcaatcac cagagaggta cggtttttct gcaactcatc cagtgccgcc tgaatcgcac
    16261 gttcggattc ggtatccaga gccgaggtag cttcatccag aatcagaatc gggctatcac
    16321 gcagcaaggc tcgagcgata gcaatacgct gacgctgacc gccagagagc agcacgccgt
    16381 tttcaccaat cactgtatcg agaccgttat ccatcttatt gatgaagtcc atggcgtagg
    16441 ccatacgcgc cgcttcttca atttgctcac ggctgtactg ttcagtccgt gcgtaagcaa
    16501 tgttgttagc aaccgtatcg ttaaacagat ggacattctg cgacaccaga gcaacctggt
    16561 tacgtaacga cgccagggta tactcgcgca gatcgtgacc atccatcagg atttcgcctt
    16621 catcaatatc gtaaaaacga gtgatcaggc tggcgatggt tgatttacct gaaccagagc
    16681 gtccaaccag agcaaccgtc ttccctgccg gaattttcag gttgatgtta cgcaatgcag
    16741 gtacgtcacg tcccggataa gtaaaggtga cattgcggaa ttccacgtcg ccagtcgcac
    16801 gctcgatcac gcgcttacct tcgtctttct cctgctcact gtccagaatg gtaaacagcg
    16861 tctgacaagc cgccataccg cgctggaact gggcgttaac gttggtcagt gatttcagcg
    16921 gacgcatcag tgcaatcatt gaagagaaaa caacggtaat cgtaccggca gtcaggctat
    16981 ccatgacact tgggaagctc gccgcataca gaacaaacgc cagcgccaaa gaggcgatca
    17041 gctgaatgat cggatcagag atggaagagg ctgaaaccat tttcatcccc tgaagacgca
    17101 ttcggttgct gactttatca aagcgtttcg tttccacttc ctgaccaccg aaaatcaata
    17161 cttctttgtg gcccttcagc atttgttcgg cgctggtggt cacctgcccc atggtgttct
    17221 gcatgttttt actgatgttg cgaaaacgct tcgatactac gcgaatcgca atcgaaacaa
    17281 tcggtgccag cacaatcaaa atgatcgaca gttgccaact gtaatagaac atcatgatga
    17341 acaggccgat gattgacgca ccttcacgca caaccgttat cagtgcgccg gaagaagaag
    17401 aagcaacctg ttcggaatcg taggtgatac gtgacaacag cgtccccgtt gactgtttgt
    17461 caaaaaatga aaccggcatt cccatcatgt gaccaaacag gcggcgacgc atggtcatta
    17521 ccacctttcc tgatacccag gagatacagt agctggagac atagctggtg ataccacgta
    17581 aaatcatcag cccgatcacc accagcggca tccacaccag cacggagcga tctgttttac
    17641 caaagccatc atcaagaagt ggcttaagga gcgataacat gaaggtatcg ctggctgcgt
    17701 tgaggattaa cgctacgccc gccacgatca gacccgcttt aaaaggcgca atggttggcc
    17761 acagtcggcg gaacgtctgc cacgtagaga gatctttgtc gttatgcatt caaaaaacca
    17821 gcatttgttg aaatagccgc atattctacc cgttatccac tggcacgcca aaccactgat
    17881 gataccaacg aggtaaaatt tgctctctga ggctgctaat ccgccagcct tgcgctgaaa
    17941 aattgaccgt cacttgaccc tgatgtggag tatcaagcca ttgatatcct tgctgttgat
    18001 agcgatgctt aactttatta gagggcaatc gccatgcgtt atagcgcgat gccgatgcga
    18061 gtgccacttt tccattcact cgctgaatta atggcaacga tgatgaggta ttactgccat
    18121 ggtgaggtac ctgaagcaat gttgtctgca cttgctgcca gtaacggctt agcatctttt
    18181 gttcagcggg gacttcaata tcaccggtta gaagaatgct attcgtcccg tcatcaacct
    18241 taaccacaca ggaatggtta tttcctttat cgttgctagc ttgtaaaggc cagtgcacgc
    18301 tgaaacgcaa tccttgccat tgccacgctt cgccacgcac acagggctga tgatgttccc
    18361 agtttaacgg acttctgatc cataacatcg gccatgtgtg caatattgag tccagccctc
    18421 cccggtgatc cagatgttca tgactcagaa taacgccttc cggttcaaga ttatgccagt
    18481 ggagccaggg gataatcagt tgttgcccac tatccccttc gggccaggcc agtcctgtgt
    18541 catagagaat cgctttgccg tttctggcta tcaccattgc cagcccttgc ccgacatcaa
    18601 gcatgtacac ctgccactcg tcaggtcgag gtttttgcca cagcggccag cacatcagca
    18661 agcctacagc cacacacatt gctggcaacg ttcgccaggc gtttaatcgc cataccacca
    18721 gtaagaacca tggggaaaat gatagccatt gccaacgttc agcaatgttg atccaccctt
    18781 ctggcaaact ctttaacccc cagaaaagta aagccaaaga ccggtcggca agaaaccata
    18841 acccttgctc caggattaac ggcccgctta aatgcacaac catcgcggcg aggatcaacg
    18901 gaaccgtgat aaatgtcacc aagggaattg ctaatagatt tgcaataaac gaggtcagac
    18961 taatgccatg aaatatgacg atttgcacag gcataagcag gagtgtgatt cccagttgca
    19021 aatgaatgag ggaaacaact gcacgcaata ccggcggcag ttgccactca ggacagggaa
    19081 accactggta ccaaaaaatc agtgccgcga ccgcagcggc agagagccat aaactttgcg
    19141 agagaatggc aacaggatcc atcagcaaaa ttgccgccag acaacatatc catacatccc
    19201 agccactcca ctgtcgccca cttaacttaa gcattcccca cgtagcaagc gccaccacgg
    19261 tacgcaatgc aggaggttgc attcccgtca accaggcata aaaagcagca cagcagattc
    19321 caccaattaa tggcatttgc cagtggatcc agcgcccagg cagataaact tgcccaccgc
    19381 gaatgagtcc ggcagccagc aacgccgcaa aagcgatgtg caatcccgat atcgccatta
    19441 aatgcgccgt tccagtatca cgcatgatat ttttgatttc tttggggacg gataaccgtt
    19501 cccccatacc taaaccaaga ataaccgcat tccacatata gggttgcagc gttgtttgta
    19561 gtgacgccag atactgtgca cgcaggctac gattcggttc aatgacactt gcctgcagaa
    19621 agcggccggt gagcggctga tgctgggcaa tggcataacg ctggctatca aagccgccat
    19681 cattaagttg gccatgaact gcacgaactt tgagtttcat tgaccattgt tgtccagcac
    19741 aaaccgcttg cggaagatat tcgccataca gcacaaggcc tggcgcaggg aatatacgtc
    19801 gaccttgtag atgagtaatt tgaccgtaat gagtggtcat gccgtcagtt gcagtgatct
    19861 caacaattgc atcctgcgtc gcgccagtta aggtttctcc tgcccacaaa atttgctttg
    19921 ctgataatat gccccaaacg aaaaacagca acgtcagcgc gatataacgg acggttttaa
    19981 cagggataaa tgccagtacg caggcgaaga gagtcagaaa cgcaagggtt aatgtcccag
    20041 gcaattgggg caaaatcagc aacggaaaaa ttccgcaaat tatgcatacg ccgactgtcg
    20101 ttattttcat gcacccctcc ctgtttatgg aaagtgtgca actttgttga gcggttgtct
    20161 ggtgggaatt ttgcgttccc cgaggcacat tcagaagaat ttaacgaaag ggtgaaaact
    20221 gctcaaaact ctgcgaggca gcttccagtt cctgattaga gaaatgaaaa aagcacccga
    20281 caggtgcttt tctcgcgttc aagtttgagt aaaaacttaa ccgtaaatat tggcgcgatc
    20341 gcgcagttct ttaccaggtt taaagtgagg aacgtatttt ccttccagtt ctactttatc
    20401 gccagtcttc ggattacgtc cggtacgtgg tgcgcggtag tgcaaagaga aactgccgaa
    20461 accgcggatt tcaatacgct cgccctgcgc aagagtcgag gccatatgct ccagcatctc
    20521 ttttactgca tcttcaaccg tcttggcggg aatgtgcgat tgctgggtgg caagtctttc
    20581 tatcaattct gacttggtca tgattcctcc ggttccttaa aggcaaatta agcggctgct
    20641 gttgctgcat tgattaaggg cggccgtagc cgcccttagt gcttgattac aggacgaaac
    20701 ctgcaatctg tcaagtaaac tcaacaaact tcggaataaa aatcccgaag agtcagagaa
    20761 ttactcgcct ttagctgctt tgaaagcttc agccattgcg ttgttggaga agtttgcatc
    20821 ttcctgtttg ttaacagttg cgattgcatc tttctcgtca gcttcgtctt tcgcacgaac
    20881 agacaggctg attgcgcggt ttttacgatc aacgccggtg aatttagctt caacttcgtc
    20941 gccaacgctc agaaccaggg tagcgtcttc aacgcggtca cgggatgctt cagaagcacg
    21001 caggtaacct tcaacgccgt cagccagttc tacggttgcg cctttagcgt caactgcagt
    21061 tactttaccg gttacgatag cgcctttctt gttcagagca acccagttgt tgaacggatc
    21121 ttctgcgagc tgtttaacgc ccagggagat acgttcacgt tctgcgtcaa cctgcagaac
    21181 aactgcagcg atttcgtcgc cttttttgta ttcacgaact gcttcttcgc ctgcaacgtt
    21241 ccaggagatg tcagacaggt gaaccaggcc gtcgatgccg ccgtccaggc cgatgaagat
    21301 accgaagtca gtgatagact tgattttacc ttcaacacgg tcgcccttgt tgtgggtttc
    21361 cgcgaactgc tgccacgggt tagctttgca ctgtttcaga cccagggaga tacgacgacg
    21421 ttcttcgtcg atatccagaa ccataacttc cactacatcg ccaacgttaa caactttgga
    21481 cgggtggatg tttttgttgg tccaatccat ttcggaaacg tgtaccaggc cttcaacgcc
    21541 ttcttcgatt tcaacgaagc agccgtagtc ggtcaggttg gtcacgcgac cagtcagttt
    21601 ggtaccttcc ggataacgtt tagcgatagc tacccacgga tcttcgccca actgtttcag
    21661 gcccagggat acacgggtac gttcgcggtc gaacttcagc actttaacag tgatttcgtc
    21721 gcccacgttg acgatttcgc tcggatgctt aacgcgtttc caggccatgt cagtgatgtg
    21781 cagcaggccg tcaacgccgc ccagatcaac gaatgcaccg tagtcagtga ggttcttaac
    21841 gataccttta acttccatgc cttcctgcag gttttccagc agctgatcgc gctctgcgct
    21901 gttttcggat tcgataacgg cacgacgaga aacaacaacg ttgttgcgct tctgatccag
    21961 cttgattact ttaaattcaa gctctttgcc ttccaggtgc agagtgtcac gcaccggacg
    22021 aacgtctacc agagaacctg gcaggaacgc acgaataccg ttcagctcaa cagtgaagcc
    22081 gcccttaact ttgccgttga taacaccggt aacagtttca gcatcttcgt aagctttttc
    22141 cagcgtgatc caggcttcgt gacgtttagc tttctcacgg gacagcagag tttcaccgaa
    22201 gccgtcttct actgcgtcca gagcaacgtc aacttcatca cctacctgga tttccagctc
    22261 gccctgggcg tttttgaact gctcagccgg gatggcggac tcagatttca gaccagcgtc
    22321 aaccagtact acgtctttgt cgatagcaac aacaacgcca cgaacgatag aacccgggcg
    22381 ggtttcgatt tcttttaagg actcttcaaa gagttgagca aaagattcag tcatgtttaa
    22441 tcttcaggtt tatatttaac gtccacctgg ctccatgccg gatggggttg tttaacatac
    22501 ccgctgtcat tccattgcaa cgggggtact gcaaattcgg tcgcttatgc gagagccaat
    22561 ttctggcgcg cgtattgtag cgctttttca atcacttgct caatgcttaa ggtggtggaa
    22621 tccaacacta aagcatcggc tgccggaacc agtggcgcta ccgctcggtt acgatcgcgg
    22681 tcgtcgcgtt ctttgatctc ggccaaaagg cgctcaaagt taacactaaa gcccttctcc
    22741 tgcaactgta gcatgcggcg atgcgcacgt tcttccgagg aggcgtcaag gaaaattttc
    22801 actggcgcat cagggaatac caccgttccc atgtcgcggc catcggcaat caggccgggt
    22861 aattcgcgaa acgcgcgttg gcgacgcaat aatgcttcac gaacgcgtgg gaatgccgcg
    22921 acttgtgaag ctgcattcgc cacttcctga gtacgaattt cgccgctgac atcttcccct
    22981 tcgaggatca cttccagatt gccattggtt gacacaaaac gtacatccag atgggatgcc
    23041 agcggtacca gcgcatcttc tgacgcaaca tcaacatggt gatgtaatgc cgccaatgcc
    23101 agtacgcgat aaattgcacc cgaatccagc agatgccatt gcaatgcttc cgccatagcc
    23161 ttacacaagg tgcctttccc tgcaccgctt gggccatcaa tggtaataac cggggcaatt
    23221 gccgtcatct ttatctcctt aaaacaggcg taccgttaac ataaccgcgc gcattatacg
    23281 cgccgacgcc acaaatcgtt aacgttgtgt gcaaaaacca gttttgtctg tattcagatg
    23341 gggatttatt gcgcaattat agcgggctgg cagaagacca gcccgaaaga tgacaatgat
    23401 ttacttacca gaggcgatac gatcacggat gtgttgcgca cgctctgttg atggtgggtg
    23461 agagtcaaac atggactggg tgcgaccacc atccagacta gccagttttt caaagctgcc
    23521 aaccagcccc tgggtgctga tgccacgttt cttcaacaga tcaaaggaga aatcatctgc
    23581 ttcggactct ttatcacggg agtatttagc attaatagcg ccttctgcga tatcacccaa
    23641 ttgtgagcgg gaaagctggg aagccacacc gctggtagct gaaatggcat cgcgtgcggc
    23701 aacgatcgca taagaagctt tcatttcagc cagcgagtga cccaacgcga cgtggcccag
    23761 ttcatggccc agaacacctt caatttcgtt gtcattcatc atgtccatca ggccactgta
    23821 gacacggaca cagccgttcg ccatcgccca tgcgttgacg tcgctggtca tataaacctt
    23881 atagttgacc ggcgtgccat taatattgtt acccaatgct ttggcgattt ttgccagacg
    23941 tttaccgtac ttgctggatt tacttgcgac ttgattgccg ctgtccattt cggcacagcc
    24001 ctgattggca atcgcttttg catcggcatc gcttaatgtt gctgctttat aggcatttaa
    24061 accggagctg atagccatat tggtatcaat accgtgggta ttttgacacc ctgtcagtaa
    24121 tgctgcagag gtcgcaatcg ccagcagtaa tttagtattc ttcatgtgaa tcgtccgttg
    24181 attaatcata tataaatatt atgtatcaac aacttacatt gacaagcgac gctaccatta
    24241 atcaaaatga ctcacaaggt ccgaaaaaaa acgccgacaa tgtgccgacg ttttgataaa
    24301 gaaagatttg gctatttatt gcccgttgtt cattcaggct gcctggctaa tccgcgccaa
    24361 ctgctcgaaa taatccggaa atgttttggc cgtacatttg ggatcaagaa tcgtcactgg
    24421 tgtatctgac aacgccacca gcgagaaaca catcgccatc cggtgatcat tgtacgtcgc
    24481 gatctcggca aagttcagtt tttccggtgg agtgatacga atgtaatcgt gcccctcttc
    24541 tacttccgca ccgactttac gcagttctgt tgccatcgca aacaggcgat cggtttcttt
    24601 aacacgccag ttatagatat tgcgcagcgt ggtggtgcct tttgcaaata acgccgccgt
    24661 ggcaatggtc atcgccgcat cgggaatatg gttcatatcc atatcaatag cgttcagttc
    24721 accacgcgtg caggaaatat aatcatcgcc ccagcaaatg gtcgcgccca ttttttccag
    24781 cacatcagca aagcgaatat caccctgcat actgttacgt ccaataccgg tcacttttac
    24841 agtgccgcct ttgattgctg ctgctgccag aaagtaagaa gccgaagatg catcgccttc
    24901 gaccaaataa gtacccggag actgataaga ctgcccgcct tttacgacaa attgttgata
    24961 gtgctgattt tcaatttcaa caccaaacgt cttcatcaga ttgagtgtga tgtcgatata
    25021 aggtttagaa accagatcgc ctttaatacg aatcaccgta tcttccggcg caagaggcgc
    25081 agtcattaac agtgcggtga ggaattggct ggaaacggag ccatcaacgt caacgttgcc
    25141 gccagtaaag ccgccctgta aacgcaacgg cggataattt tcttgttcca ggtaagtgat
    25201 cttcgccccg cccaggcgca gcgcatccac cagatgacca atcgggcgtt ctttcatacg
    25261 cggctcaccg gtcagcacaa tatcattgct acccagacaa agagctgccg ccagcggacg
    25321 cattgccgtt ccggcgttac cgaggaacaa ctccagggca ccttctgcgt gtaatggacc
    25381 gccgttaccg ataatttcgc aacgcgtacg atcggctgaa agcgtatagc ttacccctaa
    25441 cgctgttaat gcattcagca tatggcgcac gtcatcgcta tccagcagat tggttaatac
    25501 tgttttgccg tgtgctaatg ccgccagcaa taaagcgcgg ttagaaacgc tcttggaacc
    25561 gggcagatta atagtgccat cgacacgagc gatgggttgt aacgtcaggg attccatgaa
    25621 ctcaactctc tacaacagaa ataaaaaccc cacaggctgg ctgtggggat taagcaaaat
    25681 ttcggcatta accgtgacgg cgttcgaact caaccatgaa gtctgtcagc gctttaacgc
    25741 cttccagcgg catggcgtta taaatagaag cgcgcattcc gccgaccaca cggtgacctt
    25801 tcagtgcatg aaggccagca gcaaaagact cttcaaggaa caatttgtca agcgcactgt
    25861 ccgccaactg gaacggcacg ttcatccgcg aacggttagc tttcgccacg tcattgcggt
    25921 agaaatcgct gttatcaatc accccatata gcagttctgc tttttgctga ttgattttat
    25981 ccatttcagc tacaccgccg ttcgctttca gccatttaaa gaccagacca gatagatacc
    26041 aggcaaatgt cggcggcgtg ttaaacatgg agccgttatc gttgaggatg gaataatcca
    26101 gaatcgacgg acacgcgata ttcgctttgc ccagcaaatc ttcacgaacg atgacgattg
    26161 tcaggccagc cgggccgata tttttctgcg cgccagcgta aatcacgcca taacggctga
    26221 cgtcaatcgg acgggaaaga atggttgaag agaagtcggc ggcgaccacc acatctttgc
    26281 cgaagtctgg cgtttcgtcg atggcgatac cgtcgatggt ttcattcggg cagtaatgca
    26341 tataagcagc attatcagag agttgccatt cacgcattgg cttaaccgcg cgcagaccat
    26401 caacagtcac tttggcgtca aagacattag gcgtgcagta ttttttcgct tctttaatgg
    26461 cacttgccgc ccagtaaccg gcatcaacat aatctgcggt ggttttatca ccgagaatat
    26521 tcagcggtac cgcagcaaac tgaccgcgac caccgccatg gcagaataat accttgtagt
    26581 tggaggggac attaagaaga tcgcgaaaat ccttctcggc ttcctctgca acctgaatga
    26641 actctttgcc acggtgactc acttccatca ccgacgtacc aagaccgttc cagtcgcgca
    26701 gttcctgttg agcctgttta agcacctctg ccggtagcat tgccggacca gaactaaaat
    26761 tgaagatttg agccatttcc cctcaccacg ttgcgttgct attaacccgc tgtcaatcga
    26821 ttgcgaccac aggtaataca ttattcctgt ggctatcggt tttatcattc agtgacacac
    26881 atcgcaatga gtaaaaccgt tgagggcaaa aatgtggctt gcatcacaca aaagaatctc
    26941 tcggattatt agcaaaaagc cgatgttttt actgtcaggt cacccgtttt gcccgacttg
    27001 ccttcgtcca ttctggattt gcacagcgcg gacataattg tttttccccc gctttcatat
    27061 gaatgatttc actacaacga atacaggcat agtccgccga ctccggcacc actttgtaac
    27121 gctgcgtaca atcactgggt aaaataagta aacccggttt caccttgtca tcttcaaaga
    27181 aatagacact gattttcccg ttggtttcga gaatcgccag acgtacctgc cccagctgct
    27241 ccacgccacg caatcgtagc tccataaaga actcaaattc cgtcatgttg gagttattga
    27301 gtttcgacca ggccagctcg ccatcttcaa taataacaac cggcttacct tccagtagat
    27361 cttccagttt ttcactgtgc gccatcaacc acattaccag gcggtataac aacgccagag
    27421 tgataaagac gataagtacc ggcaccatcg gcacatcatc ataaaacgcc acatctcccg
    27481 ccgctgaacc cagcgtgaga atgattaaca cttcaaacag cgacatctgt cgcacaccgc
    27541 gtcttccggt cattttgagg aacaaaaaaa ccagtacaaa ggtataaaga ctacgtagtg
    27601 caacttcgcc aaggaaatca aaaggcactt tatcaaatgc catacggtgg agatcgaacg
    27661 ccttcatttt tatgctctta tcgtggttta tccgctaaaa gcatagtcaa cagttcatcg
    27721 gcatggcggc ggtgatagcg cctttgagat aaaacccgta ttattgcgcg ctttccgtac
    27781 gactaaagtg attttcatga cgcaaacatt tatccccggc aaagatgccg ctctggaaga
    27841 ttccatcgct cgcttccagc aaaaactttc agacctcggc tttcagattg aagaggcctc
    27901 ctggctgaat cctgtgccta acgtctggtc tgtacatatt cgcgacaaag agtgcgcact
    27961 gtgttttacc aacggaaaag gcgcaaccaa aaaagcggcg ctggcttctg cacttggtga
    28021 atatttcgag cgtctctcaa ccaactactt ttttgctgat ttctggctgg gcgaaaccat
    28081 cgccaacggt ccgttcgtgc attatcccaa cgaaaaatgg ttcccactga ccgaaaatga
    28141 cgatgtgcca gaaggactac tcgatgaccg tctgcgcgcg ttttatgatc cggagaatga
    28201 actgaccggc agcatgctga ttgacctaca atccggtaac gaagatcgtg gtatttgcgg
    28261 cctgccgttt acgcgtcagt ccgacaatca gaccgtttat attccgatga atatcattgg
    28321 taacctgtac gtctccaacg gtatgtccgc aggtaatacc cgcaacgaag cacgcgttca
    28381 gggattgtct gaagttttcg aacgctacgt gaaaaaccgc attattgctg aaagcatcag
    28441 cctgccagag atcccggcag acgtgctggc gcgttaccca gcagtagttg aagccatcga
    28501 aacgctggaa gcggagggtt tcccaatctt cgcttatgac ggttcgcttg gcggccagta
    28561 tccggtgatt tgcgtggtac tgttcaatcc tgctaacggt acctgctttg cctctttcgg
    28621 tgcgcatcct gattttggcg tagcactgga acgtaccgtg accgagctgc tgcaaggtcg
    28681 tggcctgaaa gatttggatg tgtttactcc gccaaccttc gatgatgaag aagtcgctga
    28741 acataccaac ctcgaaacgc actttatcga ttccagcggt ttaatctcct gggacctgtt
    28801 caagcaggat gccgattatc cgtttgtgga ctggaatttc tccggcacca cggaagaaga
    28861 gtttgctacg ctgatggcta tcttcaacaa agaagataaa gaagtttata ttgccgatta
    28921 cgagcatctg ggcgtttatg cttgccgtat tatcgtgcct ggcatgtccg atatttatcc
    28981 ggctgaagat ctgtggctcg cgaataacag tatgggcagc catttacgtg aaacgattct
    29041 ttcgctacca ggcagcgagt gggaaaaaga agattacctg aacctcatcg agcaactgga
    29101 tgaagaaggt tttgatgact ttacccgcgt gcgtgagctg ttgggtctgg cgaccgggtc
    29161 ggataacggt tggtacaccc tgcgtatcgg tgaattaaaa gccatgctgg cgctggctgg
    29221 tggcgatctg gaacaggctc tggtctggac cgaatggacg atggagttta actcatcagt
    29281 atttagtccg gaacgcgcca actattatcg ctgcctgcaa acgttgttat tactggcaca
    29341 ggaagaagat cgccagccgc tgcaatatct gaatgcgttt gttcgcatgt acggcgcaga
    29401 tgccgtagaa gccgccagtg cggcaatgag cggcgaagcg gcgttttacg gcctgcaacc
    29461 ggtagatagc gatctgcacg cgtttgctgc acatcagtcg ttgttgaagg cctacgaaaa
    29521 gctgcagcgc gccaaagcag cattctgggc aaaataaaat caaatagcct acgcaatgta
    29581 ggcttaatga ttagtctgag ttatattacg ggg
//
LOCUS       DAZFJF010000054        29536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFJF010000054 DAZFJF010000000
VERSION     DAZFJF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.054"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..960
                     /gene="ybhD"
                     /locus_tag="ACHKAU_002552"
     CDS             7..960
                     /gene="ybhD"
                     /locus_tag="ACHKAU_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5531598.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1001..1996)
                     /gene="pgl"
                     /locus_tag="ACHKAU_002553"
     CDS             complement(1001..1996)
                     /gene="pgl"
                     /locus_tag="ACHKAU_002553"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO5531599.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2151..2969
                     /gene="ybhA"
                     /locus_tag="ACHKAU_002554"
     CDS             2151..2969
                     /gene="ybhA"
                     /locus_tag="ACHKAU_002554"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO5531600.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(2970..4028)
                     /gene="modC"
                     /locus_tag="ACHKAU_002555"
     CDS             complement(2970..4028)
                     /gene="modC"
                     /locus_tag="ACHKAU_002555"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO5531601.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4031..4720)
                     /gene="modB"
                     /locus_tag="ACHKAU_002556"
     CDS             complement(4031..4720)
                     /gene="modB"
                     /locus_tag="ACHKAU_002556"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO5531602.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4720..5493)
                     /gene="modA"
                     /locus_tag="ACHKAU_002557"
     CDS             complement(4720..5493)
                     /gene="modA"
                     /locus_tag="ACHKAU_002557"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5531603.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5659..5808)
                     /gene="acrZ"
                     /locus_tag="ACHKAU_002558"
     CDS             complement(5659..5808)
                     /gene="acrZ"
                     /locus_tag="ACHKAU_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO5531604.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5937..6725
                     /gene="modE"
                     /locus_tag="ACHKAU_002559"
     CDS             5937..6725
                     /gene="modE"
                     /locus_tag="ACHKAU_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO5531605.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6793..8265
                     /gene="modF"
                     /locus_tag="ACHKAU_002560"
     CDS             6793..8265
                     /gene="modF"
                     /locus_tag="ACHKAU_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO5531606.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8526..9542
                     /gene="galE"
                     /locus_tag="ACHKAU_002561"
     CDS             8526..9542
                     /gene="galE"
                     /locus_tag="ACHKAU_002561"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO5531607.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9552..10598
                     /gene="galT"
                     /locus_tag="ACHKAU_002562"
     CDS             9552..10598
                     /gene="galT"
                     /locus_tag="ACHKAU_002562"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO5531608.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10602..11750
                     /gene="galK"
                     /locus_tag="ACHKAU_002563"
     CDS             10602..11750
                     /gene="galK"
                     /locus_tag="ACHKAU_002563"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO5531609.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11744..12784
                     /gene="galM"
                     /locus_tag="ACHKAU_002564"
     CDS             11744..12784
                     /gene="galM"
                     /locus_tag="ACHKAU_002564"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO5531610.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFLGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            12987..13739
                     /gene="gpmA"
                     /locus_tag="ACHKAU_002565"
     CDS             12987..13739
                     /gene="gpmA"
                     /locus_tag="ACHKAU_002565"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO5531611.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13898..14950)
                     /gene="aroG"
                     /locus_tag="ACHKAU_002566"
     CDS             complement(13898..14950)
                     /gene="aroG"
                     /locus_tag="ACHKAU_002566"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO5531612.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15266..15646
                     /gene="ybgS"
                     /locus_tag="ACHKAU_002567"
     CDS             15266..15646
                     /gene="ybgS"
                     /locus_tag="ACHKAU_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO5531613.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15760..16701
                     /gene="zitB"
                     /locus_tag="ACHKAU_002568"
     CDS             15760..16701
                     /gene="zitB"
                     /locus_tag="ACHKAU_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO5531614.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16698..17417)
                     /gene="pnuC"
                     /locus_tag="ACHKAU_002569"
     CDS             complement(16698..17417)
                     /gene="pnuC"
                     /locus_tag="ACHKAU_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO5531615.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17455..18498)
                     /gene="nadA"
                     /locus_tag="ACHKAU_002570"
     CDS             complement(17455..18498)
                     /gene="nadA"
                     /locus_tag="ACHKAU_002570"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO5531616.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18747..18871)
                     /locus_tag="ACHKAU_002571"
     ncRNA           complement(18747..18871)
                     /ncRNA_class="other"
                     /locus_tag="ACHKAU_002571"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(18903..18978)
                     /locus_tag="ACHKAU_002572"
     tRNA            complement(18903..18978)
                     /locus_tag="ACHKAU_002572"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18943..18945),aa:Lys,seq:ttt)
     gene            complement(19012..19087)
                     /locus_tag="ACHKAU_002573"
     tRNA            complement(19012..19087)
                     /locus_tag="ACHKAU_002573"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19052..19054),aa:Lys,seq:ttt)
     gene            complement(19234..19309)
                     /locus_tag="ACHKAU_002574"
     tRNA            complement(19234..19309)
                     /locus_tag="ACHKAU_002574"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19274..19276),aa:Lys,seq:ttt)
     gene            complement(19313..19388)
                     /locus_tag="ACHKAU_002575"
     tRNA            complement(19313..19388)
                     /locus_tag="ACHKAU_002575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19353..19355),aa:Val,seq:tac)
     gene            complement(19538..19613)
                     /locus_tag="ACHKAU_002576"
     tRNA            complement(19538..19613)
                     /locus_tag="ACHKAU_002576"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19578..19580),aa:Lys,seq:ttt)
     gene            complement(19616..19691)
                     /locus_tag="ACHKAU_002577"
     tRNA            complement(19616..19691)
                     /locus_tag="ACHKAU_002577"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19656..19658),aa:Val,seq:tac)
     gene            complement(19827..19902)
                     /locus_tag="ACHKAU_002578"
     tRNA            complement(19827..19902)
                     /locus_tag="ACHKAU_002578"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19867..19869),aa:Lys,seq:ttt)
     gene            complement(20067..20858)
                     /gene="cpoB"
                     /locus_tag="ACHKAU_002579"
     CDS             complement(20067..20858)
                     /gene="cpoB"
                     /locus_tag="ACHKAU_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO5531617.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20868..21389)
                     /gene="pal"
                     /locus_tag="ACHKAU_002580"
     CDS             complement(20868..21389)
                     /gene="pal"
                     /locus_tag="ACHKAU_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO5531618.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21424..22716)
                     /gene="tolB"
                     /locus_tag="ACHKAU_002581"
     CDS             complement(21424..22716)
                     /gene="tolB"
                     /locus_tag="ACHKAU_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO5531619.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22849..24114)
                     /gene="tolA"
                     /locus_tag="ACHKAU_002582"
     CDS             complement(22849..24114)
                     /gene="tolA"
                     /locus_tag="ACHKAU_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5531620.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(24179..24607)
                     /gene="tolR"
                     /locus_tag="ACHKAU_002583"
     CDS             complement(24179..24607)
                     /gene="tolR"
                     /locus_tag="ACHKAU_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO5531621.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24611..25303)
                     /gene="tolQ"
                     /locus_tag="ACHKAU_002584"
     CDS             complement(24611..25303)
                     /gene="tolQ"
                     /locus_tag="ACHKAU_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO5531622.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25300..25704)
                     /gene="ybgC"
                     /locus_tag="ACHKAU_002585"
     CDS             complement(25300..25704)
                     /gene="ybgC"
                     /locus_tag="ACHKAU_002585"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5531623.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25854..26147)
                     /gene="ybgE"
                     /locus_tag="ACHKAU_002586"
     CDS             complement(25854..26147)
                     /gene="ybgE"
                     /locus_tag="ACHKAU_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO5531624.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(26147..26260)
                     /gene="cydX"
                     /locus_tag="ACHKAU_002587"
     CDS             complement(26147..26260)
                     /gene="cydX"
                     /locus_tag="ACHKAU_002587"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO5531625.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26275..27414)
                     /gene="cydB"
                     /locus_tag="ACHKAU_002588"
     CDS             complement(26275..27414)
                     /gene="cydB"
                     /locus_tag="ACHKAU_002588"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5531626.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27430..28998)
                     /gene="cydA"
                     /locus_tag="ACHKAU_002589"
     CDS             complement(27430..28998)
                     /gene="cydA"
                     /locus_tag="ACHKAU_002589"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO5531627.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7355 a         7792 c         7269 g         7120 t
ORIGIN      
        1 gtaataatga agcatgaatt atcaagtatg aaggcatttg tcatactggc agagtccagt
       61 tcatttaaca atgctgctaa attactcaat attacgcaac ctgcattaac gcgaagaata
      121 aaaaagatgg aggaagattt acatattcaa ctatttgagc gtacaactcg caaggttacg
      181 ttaacaaaag caggaaaaag gttgctcccg gaggcgcggg aattaataaa gaaattcgat
      241 gaaacgcttt ttaatattcg tgatatgaat gcttatcatc gtggtatggt gacgttagcg
      301 tgtattccga ccgcagtatt ttatttttta ccgctggcaa tcggtaaatt taatgagctg
      361 tatcccaata ttaaagtgcg gattctggaa caaggcacga ataattgcat ggaatcggtg
      421 ctgtgtaacg aatcagactt tggcataaac atgaacaacg tcacaaattc atccattgat
      481 tttaccccac tggtcaacga gccattcgtg ctggcctgtc ggcgtgacca tccattggcc
      541 aaaaagcagc ttgtagaatg gcaggagtta gtgggttaca aaatgattgg cgtacgttcc
      601 tccagcggca accgattgtt gatagaacaa cagctagccg acaagccctg gaagctggac
      661 tggttttacg aagtgcgtca tctttcgacg tcattaggac tggtagaggc gggactgggg
      721 atttcggcgc ttcctggtct ggcaatgccc catgcgccct attcttcgat tattggtatt
      781 ccactggtgg aacccgttat acgtcggaca ttggggatta ttcgccgcaa agatgccgtg
      841 ctttctccgg cagcagaacg cttttttgcc ttgctgatta atttgtggac tgacgataaa
      901 gacaatttgt ggaccaatat cgtcgaacgc cagagacatg cgttacagga gataggttaa
      961 tacaccggcg caggagagac tgcgccggtt aaatcagcgg ttagtgtgcg ttaaccacca
     1021 cccacattgg tccctgcccg accgcataac ggcctttttc atgcagtagt ccctgctcgc
     1081 caacaatttc gtataccgag atgtggtgag atttttgccc ggcggcaatc agatacttgc
     1141 cgctgtgatc aacattgaag ccgcgcggct gggtttccgt tggctggaag ccttctttac
     1201 tcaacacgct gccatcttcc gaaacgctga aaacggtaat caggctggcg gtacggtcgc
     1261 aggcgtataa atggcgacca tccggggtga tatgaatatc agccgcccaa cgggtgtcgg
     1321 agaagttttc cggcatcata tccagcgtct ggacacattc gatattaccg tgcggatctt
     1381 tcagttccca gacatccact gagctgttta actcattgac gcaatacgca tattgttcgt
     1441 ttggatggaa taccatatga cgcgggccgg ccccttcaac ggtggtcact tccgcagggt
     1501 cctgcgccac gagatgacca tcatcgctga ccgtaaacag gcaaatgcga tcctgcttta
     1561 atgccggaac ccacagcgta cggttgtccg gtgagatatt ggcggaatgg caaccgtcca
     1621 gcccctcgac cacatcgacg acgcccactg gcaggccatc ttccagacgc gttacgctca
     1681 cgttacccgc attgtaagaa cctacaaaga caaactgccc ctggtgatcg gtggaaatat
     1741 gcgtcggact acccggcagc gcagactctg cggcaaaggt cagtgcgcca tcgtccgggg
     1801 cgatactata cgccaggacg cgaaactcag ggcgaacacc aacatagaga taacgtttgt
     1861 ccgggctgac caccatcggc tgcacctgcc ccggcacatc gacaacctgt gtcagcgtca
     1921 gtgcgccttc atgattcagg ttccagacgt gaatttgctg gctctcaggg ctggcgatat
     1981 aaactgtttg cttcatgaat gctcctttgc atttagcaac tgactgtaac agctaaaatt
     2041 agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat agtggtatcg
     2101 gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc atgaccacac
     2161 gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc ctgcttcctt
     2221 catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag
     2281 gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat acacctgcta
     2341 tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg gaagcggacc
     2401 caatgcccgt taataaagcc ctgcaactca ttgagatgct gaatgaacac cacattcacg
     2461 gtctgatgta tgtggatgat gcaatggtct atgagcaccc gaccgggcat gtcattcgca
     2521 cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca caagtcgctt
     2581 ctctggctga aacggcgcaa caagttaacg ccgtatggaa gttcgccctg acgcacgatg
     2641 acctgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga ctggagtgtg
     2701 aatggtcctg gcacgatcag gttgatattg cacgcggcgg caacagcaaa ggtaaacgtt
     2761 tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa cgtcgtggcg ttcggcgata
     2821 actttaatga tatcagtatg ctggaggccg ctggtacagg cgtggcgatg ggcaacgccg
     2881 atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc gacagcattg
     2941 cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact tttaatttgc
     3001 gcgtacagcc acaggccagg tttgatcacc agttcatccc tggcccacgg gctgatacgc
     3061 gcccacagcg ttttacctcc gacttccagt tccacttcca cctggccgtt gtcgtcataa
     3121 ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg cggttgtaaa
     3181 accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag cggttcgtcc
     3241 agcttattga cccacaaatg ctgatcgccc agcgccagcg cagtcatcgc gtaatgcgga
     3301 tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg cagccacgga
     3361 ttcatcacgc tactacccca gacttcttcc agtgcgccaa aggctttcac ctgaccgttt
     3421 tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg gctgacatac
     3481 aacatcggga tgttgatttc ccgcgtcaga cgttgcagat aaggcaacag ttcgcgttta
     3541 cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattctgg tgccgtcagc
     3601 aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg acaggctgcc tggtaaacgg
     3661 tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc gaccatgctt
     3721 ttcgccatgc cgtagcgcag attgccacgc actttgtaat gcggaaacag ccgcgcatcc
     3781 tgaaaaacat agccaacgcg acgcttttct ggcgtcaggc agataccttt ttcggcatca
     3841 tttagtaccc gaccattgag gacaatcctc cctttttgcg ggcgcgtcag tccactgatg
     3901 gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt gatgccattg
     3961 gcgggcagcg tttcattaat ggtcaggcaa tggttgccca aggtctggga aaaattcagt
     4021 tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat tctgaaatca
     4081 acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg ccactttccc
     4141 cgccgggggt ctggatcagg gtatacatgg cagaaggaat ggttcgcgtt tcaccaggaa
     4201 tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca aaagccagta
     4261 ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag aaaacgcgcc
     4321 agcgcccggc ccccagtgtt cttgcggcct gttcgagttt gacatcaacc ccttccagcg
     4381 ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg agaaccgcgc
     4441 cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca ccgataaatc
     4501 cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt aacaccagcg
     4561 gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc accagtaacc
     4621 aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa actttcaggc
     4681 ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg taaatccgta
     4741 acgtttaaag atttccgcag cctgcggccc tttcaaataa tcataaaatg ctttcactgt
     4801 cgcattgtta tgctcttcca caacagcaac cggatattcc acttttttat gtgaatcttc
     4861 cgggaaggtg gcaaccactt ttaccccttt gctggcaact gcgtcagaac cgtaaacaat
     4921 gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat cttccgccgg
     4981 agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt ctttcgcata
     5041 aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat tcagcagcga
     5101 agtccagttg gttttgctgt cgatggtgaa atctttctgc tcgctggctt tcggtgctac
     5161 aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg cttttttatc
     5221 aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag gcgctcccgc
     5281 ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat ccacgccttt
     5341 ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg cggcggcgaa
     5401 caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag agagtgccgc
     5461 cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt aacgttatgt
     5521 aggcgacaat ataacggcta gctcagaatt ttcccagcag ttattgctaa cctttgtgag
     5581 gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa atgcccgact
     5641 aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac caacaccaga
     5701 aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg ccatcacgac
     5761 aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg tcaactccag
     5821 atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct tttgtgcggt
     5881 cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac attgttatgc
     5941 aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac ccgcgccgga
     6001 tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg aaagatgccg
     6061 gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta agtgagcata
     6121 ttctggtcga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg acccgctatg
     6181 gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg
     6241 tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc tcacgttttt
     6301 cactgcaaac cagcgcccgt aaccagtggt tcggcaccat caccgcccgc gatcatgatg
     6361 acgttcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa
     6421 ttaccgcaca aagcggcgcg cgtctggggc tggatgaagg caaagaagtg ttgatattgc
     6481 taaaagcgcc gtgggtaggt attactcagg acgaggcggt cgcacaaaac gccgacaacc
     6541 aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa gtattaatgg
     6601 cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc
     6661 agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc gccacgctgt
     6721 gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc gctgcacaaa
     6781 atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca
     6841 aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc gtttgtcggt
     6901 tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact tccgcttttg
     6961 aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga gcaattgcaa
     7021 aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc tggcgaagat
     7081 gacaccggac gcactacggc tgagatcatt caggatgaag taaaggatgc accgcgttgc
     7141 atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt taaatacctt
     7201 tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg
     7261 ttgattcttg atgagccgtt cgatggcctg gatgttgcct cacgtcagca gctggctgag
     7321 cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa tcgcttcgat
     7381 gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt agcggaaact
     7441 ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca tagtgaacag
     7501 cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc cttacccgcc
     7561 aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg ccccattctt
     7621 aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg gccaaatggt
     7681 gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg ttacagcaac
     7741 gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga tatcaaaaag
     7801 catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac taccgtgcgt
     7861 aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt ttcggatcgc
     7921 cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg tacggctgac
     7981 gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg
     8041 gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga tccgctcaat
     8101 cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg
     8161 tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct tgagttcgtg
     8221 ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg tagtgctgac
     8281 cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg caggcatgaa
     8341 accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt ttataatcac
     8401 ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa tttattccat
     8461 gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc ctaatggagc
     8521 gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat acctgtgtgc
     8581 aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac agtaagcgca
     8641 gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata
     8701 ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac accgtgatcc
     8761 acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa tattacgaca
     8821 acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact
     8881 ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca tacgttgaaa
     8941 gcttcccgac cggcacacca caaagccctt acggcaaaag caagctgatg gtggaacaga
     9001 tcctcaccga tctgcaaaaa gcccagccgg actggagcat tgccctgctg cgctacttca
     9061 acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc attccgaata
     9121 acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg gcgatttttg
     9181 gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac gtaatggatc
     9241 tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc gtacacatct
     9301 acaacctcgg cgctggcgta ggcaacagcg tgctggacgt ggttaatgcc ttcagcaaag
     9361 cctgcggcaa accggttaat tatcattttg caccgcgtcg cgagggcgac cttccggcct
     9421 actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg
     9481 atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga tatcccgatt
     9541 aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc tacaacccgc
     9601 tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc
     9661 aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc ctctgcgcag
     9721 gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac caggacttac gttttcacta
     9781 atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac gatcctttga
     9841 tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg gatcacagta
     9901 aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc
     9961 aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg
    10021 cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc ttcctgccta
    10081 acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa
    10141 tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg
    10201 aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg ctactgctgc
    10261 ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc
    10321 tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc ttcccctact
    10381 ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg cagctgcacg
    10441 cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg gttggttatg
    10501 aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg
    10561 cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa
    10621 cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt caggcgcctg
    10681 gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt ctgccctgcg
    10741 cgattgatta tcaaaccgtg atcagctgtg cgccacgcga tgaccgtaaa gttcgcgtaa
    10801 tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc attgtcgcgc
    10861 atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg caactgcgta
    10921 acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt
    10981 taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag ctttattatc
    11041 tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac cagtttgttg
    11101 gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa gatcatgcct
    11161 tgctgattga ctgtcgctca ctggggacca aagcagtttc catgccgaaa ggtgtggctg
    11221 tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac aacacccgtc
    11281 gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agccctgcgc gatgtcacca
    11341 ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa cgcgtgcggc
    11401 atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag cagggcgacc
    11461 tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat gatttcgaaa
    11521 tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agctgtgatt ggcgacaaag
    11581 gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgttg atcccggaag
    11641 agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa acaggtatta
    11701 aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga acgaaactcc
    11761 cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca acgcagggat
    11821 ggtagtcacg ctgatggact ggggtgcgac tttactttca gcccgtattc cgctttccga
    11881 tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc aggatcaggc
    11941 cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata gccgttatac
    12001 ctttgacggt gaaaccgtga ccctttcgcc aagtcagggc gttaaccagc tgcacggcgg
    12061 cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc gtcaggtgct
    12121 gtttgccctg agttcagatg atggcgatca gggcttcctg ggtaatctcg gcgcaacggt
    12181 gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca cagttgataa
    12241 accttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg agcagtctga
    12301 cgtgcgcaat cacaagttgc agattctggc ggacgaatat cttccggttg atgaaggcgg
    12361 cattccgcac gacggcttga aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa
    12421 aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag gttacgatca
    12481 cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc
    12541 agatgaaaaa ttgcagctga aggtctacac caccgctccg gctcttcaat tctactccgg
    12601 caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact ggcaagggct
    12661 ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc cgcaaccgga
    12721 ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt ttattgctga
    12781 gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc accagcaaac
    12841 accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact attttcgcta
    12901 tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa ttattatcat
    12961 taaaagatga tttgaggagt aagtatatgg ctgtaactaa gctggttctg gttcgtcatg
    13021 gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg
    13081 agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa ggttacagct
    13141 ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg
    13201 acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac gaacgtcact
    13261 acggtgcgtt gcagggtctg aacaaagcag aaaccgctga aaagtatggc gacgagcagg
    13321 tgaaacagtg gcgtcgtggt tttgcagtca ctccgccgga actgactaaa gatgatgagc
    13381 gttatccggg tcacgatcca cgttacgcga agctgagcga gaaagaactg ccgctgacgg
    13441 aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact attctaccgc
    13501 gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta cgtgcgctgg
    13561 tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc ccgaccggcg
    13621 tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat tatctgggta
    13681 atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa gcgaagtaaa
    13741 cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag ccggcttttt tgttgctaac
    13801 aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg
    13861 gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg
    13921 cattcgccag ttgacgtaac agagcatcgg tatcttccca gccgatgcag gcatcggtga
    13981 tgctcttacc gtaggccagc ggctccccgc tctcgaggct ctgattgcct tccaccagat
    14041 ggctttccac catcacgcca ataatggctg tttcgccacc ggcaatctgc tggcaaacgt
    14101 cagcacacac atccatctgc tttttgaatt gtttggacga gttagcatgg ctgaaatcga
    14161 tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca gcaacgtgct
    14221 tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca ccgttaccgc
    14281 tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac
    14341 cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac
    14401 ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcg gtggtacgtg
    14461 caccaattgc gccccagctc atcaggtcag cgagatattg tggggtgatc atatcgagaa
    14521 actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac
    14581 gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc agccctttcc
    14641 agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt
    14701 tcagctcttc acgcagcgcc agcaagcgag tggcatactc ttttgctgcg acaggatcat
    14761 gaattgagca tgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat
    14821 ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagtagcg gggaattttt
    14881 ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct
    14941 gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat
    15001 ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga
    15061 attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact
    15121 gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata acctggctga
    15181 aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac
    15241 ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac
    15301 tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa
    15361 taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa
    15421 tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggtg gcacaatgct
    15481 gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa
    15541 caccatgtgc aaagatggtc gctgtccgga tatcaataaa aaggtacaaa ccggtgatgg
    15601 catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc
    15661 caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg
    15721 tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac
    15781 acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg
    15841 actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg
    15901 ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc
    15961 gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc
    16021 acgctcgcgg cgtttgtgaa tgccatagcc ctcgtggtaa ttaccatttt gattgtctgg
    16081 gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat gatggcaatt
    16141 gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa
    16201 gagaaaaacc tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg
    16261 gttggggcta ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc
    16321 attctctcga tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat
    16381 agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt
    16441 cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta
    16501 ggcgagaagc cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc
    16561 ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt
    16621 cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc
    16681 cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg
    16741 atccacatcc ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg
    16801 gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac
    16861 agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc
    16921 atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat
    16981 cctaatgctt gcatgatcat gactgccagg cgggtcaaaa atgcaaacac cggattgata
    17041 aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc
    17101 aatggcaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca
    17161 taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc
    17221 tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa gaagtagttg
    17281 ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct
    17341 tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag gatattctgc
    17401 acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc
    17461 acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag
    17521 cctttcatca acatgaacct cgtgattgct tccttcctgt tctaatgcct ctgcgatgga
    17581 ctgaaggcca ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcaccctc
    17641 acctgcggtt ggtgcttcca gtaactcttt atctggcact gcctgctgca ttttgtagaa
    17701 aatgccccga tcggttgcca cgataagccg ctgatgtggc aatgttttcg cagcagcgat
    17761 cagttgactg gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg
    17821 atgcaccagt atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt
    17881 aaattcatca tgcacaatac aggcaccctg ccagcataga atgtctccac ccgtctgttt
    17941 ttgcacgtaa cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc
    18001 aagatgatca ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt
    18061 taccgcagca gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa
    18121 tgcgttaaat tcttcaacag ggcagccgag atccagtgaa cattcagcct gaagtgtcgg
    18181 catcagaatt gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc
    18241 agcgactaac aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc
    18301 agaaatacag ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg
    18361 ggcaaccatc accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc
    18421 tttttcatca atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg
    18481 atcaaacatt acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt
    18541 agcgtaatat tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat
    18601 ggtggggaaa agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt
    18661 aagctctgac gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa
    18721 agcgccttta gggcgctttt ttacattggt gggtcgtgca ggatgactcg cttcgctcgc
    18781 ccttcgggcc gtcgccgcga gcggctccgt tgtttcgctg cgctcaactc aaacctgctg
    18841 caggttcgaa tctttaagta tcgcagatag caaaaaagcg cctttagggc gcttttttac
    18901 attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac
    18961 caactgagct aacgacccct tgcgggatta tgctgcttta atcattcagg atggtgggtc
    19021 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
    19081 acgacccata cgggtgttac ctgaaagatt tctactcggg gccaatcttt aagattggtg
    19141 tgtcgtgcag gatgactcgc ttcgcttgcc ctacgggccg ttgctggcgc aacgttatcc
    19201 ttcacgctta acatctgagt ttgatgttaa aattggtggg tcgtgcagga ttcgaacctg
    19261 cgaccaattg attaaaagtc aactgctcta ccaactgagc taacgacccg agtggtgggt
    19321 gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct
    19381 aatcacccga tactgcgctg gatactacaa actacttcca ccgaacccac cgtaattagt
    19441 ggtgggtcgt gcaggatgac tcgcttcgct cgccctacgg gccgttgctg acgcaacgtt
    19501 atcctacacg cttaacatct gtcttcgatg ctaaaactgg tgggtcgtgc aggattcgaa
    19561 cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga cccggtggtg
    19621 ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggagatgctc tcccagctga
    19681 gctaatcacc caatactacg ctggatacta caactaacta tgttagtggt gggtcgtgca
    19741 ggatgactcg acttcgtctc gcccttcggg ccgttgctga tgcaacgtta tccttcacgt
    19801 tttactaccg cttccacctt agcgattggt gggtcgtgca ggattcgaac ctgcgaccaa
    19861 ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccactttttc gttgctttcg
    19921 gtttgtttga tatcccgtgg caacggcggc atatattact gatttcagac ttgagcgcaa
    19981 caaaaatttc gatgcagatc actcaactgc ttatgattcg cacgacacga ccagaaataa
    20041 tgcgacttct ggtcgtgtgt tatgcattac atcgcgttca gacgtttttg tgcctgttta
    20101 gcgccatcag taccagggta tttactgata acctgctggt atacggcttt cgcttttgcg
    20161 gtgtcacctt tgtcctgcat gatgacgccg actttaaaca tcgcatcagc agcctttggt
    20221 gacttcggat agtttttcac taccgaagca aaatagtacg ccgcatcatc ttttttaccc
    20281 ttgttgtagt ttaactgacc cagccaataa ttggcgtttg gcaggtaagt tgaatcaggg
    20341 taatttttga tgaaattctg aaatgccacc attgcgtcat cctggcggga tttatcctgc
    20401 accagcgcaa tagctgcatt gtaatccgtg tttgcatcgc cgcttttcac cggcgcgcca
    20461 gcattcgcag taccagcatc agctgtcggc gtcgttgatg ccgccgcacc gctttgatcg
    20521 ctgctggttg attgcgccgc tgcaccaccg ctgctgaggc tgtcgatctg caacaggatc
    20581 tgcttttgcc gctccacgac ctgattcagt tgatactgat tttcctgaat ctgaccacgc
    20641 agggaatcaa tatcggattg attatcagaa agttgttgct ggagttgggt taaaagctgg
    20701 ctgtgagcat tagaaatacg ctcaagttga gtgacgcggt cttcgaccga gcctgagccg
    20761 acactactga ttggtgcctg agcaaaagcg gcccaggggg ccgctatacc aaccagtaac
    20821 gacagactca atagttgatg tctgaagtta ctgctcatgc aattctctta gtaaaccagt
    20881 accgcacgac ggtttttgga gtatgccgct tcgtcatgac ccagtactgc aggtttttct
    20941 ttaccgtaag aaacgatgga gatctggtct gcagaaacgc ctttaccctg caggtacatc
    21001 ttaacggcgt tcgcacgacg ttcacccagg gagatgttgt attccggagt accacgttcg
    21061 tccgcgtgac cttctacggt gactttgtaa gacgggttgc tacgcaggaa gtttgcatgt
    21121 gcatccagca tttgagcgaa gtcagaacgg atatcgtact tgtccagatc gaagtaaacg
    21181 atgttgttct gctgcagctg ttgcatttgc agacgagcct gctcttcgga agacatgttg
    21241 ccgttgccgc cgttcgcatc cataccagtg ccggcaccca gcatgccttc gctgccgtca
    21301 ttgctggcgt tcttgttgga agaacatgcc gcaattgcca taacaggcag agcaatcatc
    21361 agccctttca gcactttgtt cagttgcatt tcaatgattc ctttactatt caattaatta
    21421 ttatcacaga tacggcgacc aggcagggaa tttgacctgt ccatcagttg ccggaagacg
    21481 cgctttgaaa cgcccatctg tagaaaccaa attcagcacg gatcccatcc cctgagaaga
    21541 gctgtagatt accatagtgc cgttaggtgc cagacttggc gtttcatcca ggaacgtgga
    21601 cgacagaact tgtacgcctc ccgttgccag atcttgtttg gcaatgtgct gctgcccacc
    21661 attggagctg accattacca taaatttacc gtcgctgctg acatccgcat cctggttctg
    21721 cgaaccttcc caggtaatac gttgtggcgc accgccgttg atattcactt tataaacctg
    21781 cggacgaccg gcctggtcag aagtaaatgc caggttctgg ctatccggga accaggtcgg
    21841 ttcggtattg ttactgcgac catcagtcac ctggcggatc tgaccagaag ccaaatccat
    21901 tacgtacagg ttcagactac cggttttcga caaggcgaat gccagtttgc tgccgtctgg
    21961 cgagaatgca ggtgcaccgt tgtgacgcgg gaatgaagcc acctgacgta cagcgccatt
    22021 tgccagcgtc tgaataacca gcgcggaacg accgctttcg aaggtcacat aagccagttt
    22081 agaaccgtct ggtgaccacg ccggagacat cagcggctgc ggtgaacggt gaacgacaaa
    22141 ctggttgtaa ccgtcatagt cagatacgcg cagttcatac gggaactgac cgccgttggt
    22201 ctgaacaacg taggcaatac gagtacggaa cgcaccttta atgccggtca gtttttcaaa
    22261 cacttcatca ctggcggtat gaccagcata acgcagccac tgcttgttca ctttgtacga
    22321 gttctgagca agtacagtac ccggtgcgcc gccagtgtca acaagttgat aagcaacatt
    22381 gtaagaacca tccggattcg gagtgacctg accgacaact acagcgtcaa tgcccagtgc
    22441 ggaccatgca gctggttgta cttcctgcgc actacccggc tgctgtggca gacgagcgcg
    22501 atctaacgga ttaaatttac cgctgttacg caagtcagca gcaacgatgc cgccaatatc
    22561 ttcaggtgcc gcaccaggcc ccgcccactg gaaaggaaca acaccaatag gacgaccgga
    22621 atctacaccg ctgtcgatca caatgcggac ttcagcatgc agaactgatg cccacagtat
    22681 gagaaaacca aatgctactc gtaatgcctg cttcatcata tctcccttat ctggaccgat
    22741 ggcccacgat aatttagcag aatgttaaca aactaaaata cacaaaacta ccagaacccc
    22801 gtggcaaccg gtgcctgatg ttgaccgtcc gaacagtcaa catcgcgatt acggtttgaa
    22861 gtccaatggc gcgtttttga acacttcata tactgcctgg cttggtggtt tcgggatctt
    22921 cgcaagttta gctgctgcca acgcagcctg acaaagtgcg ggatcgccac cttcaggttt
    22981 gatatccagt aacataccat cgggtgccag ttttatgcgc agcgtacagg ttttgcctgc
    23041 ataggacgat gcgtcataga acttactttc gatagcagat ttaatctgcc cggcatagtt
    23101 attgatatcg gcccctgatg cgccattgtt tttagtatta ccactcccgg caggcgaagc
    23161 attgttccct ttcgcccctc cccccgtttt cggtgcattc ttaccagagc ttagctcacc
    23221 gaaaatatca tctgcctctg cggccgcttt tgctgcagcg gctttttcag ctgcagcttt
    23281 tgccgctgcc gcttttttgt ctgcggcggc tttttctgcc gctgctttct tatcagctgc
    23341 tgccttttca gcagccgctt tttctgctgc ttttttgtcg gctgcagctt tctctgctgc
    23401 cgctttctta tcagctgcag ccttttcagc agccgctttc ttctcagctt ctgctttggc
    23461 tttttcagca gcttcagttg ccgctttctt tcttgcttca gctgcagctg cttctgccgc
    23521 ttccgctttc ttcttcagtg ccgcagcggc tgcctcggct tttttctgcg cttcggctgc
    23581 ggctttggcg gcttctgctt ctgctttttt ctttgcgtct gcagccgctt tcttcgctgc
    23641 ttcttccgca gctttagcat ctgcttcggc cttcgcttta gcatctgccg ccgctttcgc
    23701 tgccgcctct tcagcttgct tctgctttaa ctcggcctgt tttgcggctt cttcagcctg
    23761 ctttttctgc tcctgagccg ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc
    23821 agccgcttgt ttctcacgca gttcttcagc agcctgctgt tccttcatct tgcgctgttc
    23881 atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt ttgtactgct caactaccgc
    23941 acctgaatca accatgacag cgtcgatgga cgaaccaccg ccgcctccgg ctgaagcttc
    24001 tatattctca tcgaacgaac tccagatcag cgccgcaaat aagatgacat gcagcactgc
    24061 tgaaataatt atcgcccgct tgagcttgtc gttttgttcg gttgcctttg acactctcgg
    24121 tttccaaaaa ctgttcgcct gttacccgct ctctttcaag caagggaaac gcagatgttt
    24181 agataggctg cgtcattaaa ccaaccgatt tcacacccgc actatgtaac aagttcagtg
    24241 ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc gatcagaaag accgttttcg
    24301 ggttggcctt gaaacggctg gacacttccg ccaccacctg ctctggtggt aaacgctcca
    24361 ggcgatcttt ctcaaccacc acggtgtact gaccaatacc agacacttca acaatcactg
    24421 gcggattatc gttactgctc accgcctgtg attcagtagc gtctggcaga tcgacctcca
    24481 cgctctgggt gatgatgggc gctgtcgcca tgaagatcag caacagcacc agcagtacgt
    24541 ccagcaacgg tacaatgttg atttcggact taagttcgcg acgacctcgt ccacgcgctc
    24601 tggccatgac ttacccctta ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg
    24661 cggtaaactc ttccataaag ttgtcgtaat tcagttccag tttgtttacg cgctggttga
    24721 ggcggttgta ggccataact gccgggatag cggcaaacag accaattgca gtcgcaatca
    24781 acgcttctgc gataccgggc gcaaccattt gcagtgttgc ttgttttacc gccccgaggg
    24841 cgataaaggc gtgcatgatc ccccagaccg taccaaacag accaatatac gggctgatgg
    24901 agccaaccgt accgaggaac ggaatgtgcg tttccagatt ttcaagttca cggttcatgg
    24961 agatacgcat agcacgcgac gccccttcca ctacggcttc cggcgcatgg ctattggcac
    25021 gatgcaggcg cacaaactct ttgaacccgc tgtaaaatat ttgttccgaa ccagtcagat
    25081 tatcccgttt cccctgactc tcttgataga ggcgagagag ttcgattcca gaccagaatt
    25141 tatcttcaaa cgcttcggct tcgcgcgccg ctgcgttaag aatacgggtc cgctggataa
    25201 taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa
    25261 ggctagcctt caggaacaaa tcaaggatat tcatgtcagt cactgcttaa actccgcgac
    25321 aatagacttg ggaagcgcac gaggcttcat tttgagtggg tcaacgcaaa caaccagaac
    25381 ctctgcttca ttcagcaaag tattctcggc gttgacaata cgttgcgtga aaaccaaaga
    25441 ggtgccacgc attgatgtta tttcagtctg tatttcgagc atatcgtcga gccgcgcagg
    25501 tgcgtaatat tccaccgtca ttttacgtac cacaaaggca acgcgttcag ccatcagcgc
    25561 ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac
    25621 gtaactggcg tggtacacca caccaccggc atcggtatct tcatagtaga cgcgaaccgg
    25681 ccatcgaaac agcgttgtat tcactttaca tcccggtaat gcaacaaaag ttagagcttt
    25741 taaacgctgc tactatacgc gcgcgattga ggtttgggaa tgagggcaag ttaagggagc
    25801 gtaaaaattt atgggcctgg agcggcccat aattttttaa agagaattct gatttaaaag
    25861 aagaagaaaa tcagcccgac aatgagaaca atatcggcaa gcaatgggca aaaaatccct
    25921 tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa tcacaccagc acagacggcc
    25981 cacatcagca ataaaccatg ccagatttcc agttcactgg tcttcgcggc aaaacgagat
    26041 gggtcccaaa acatacatcc tgctaacaga agcgccatca cgaaggaaag cgcccgcagg
    26101 gggcgcttgt ccattaccgc atacaaagtc gcgataatct tactcatcag atgtcttctt
    26161 gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc ggtgattacc ccaaacgaac
    26221 aggcaagaag cgttcccaga atccatgcga aataccacat ttttagctcc ttacttagta
    26281 cagagagtgg gtgttacgtt caatatcttc tttggtgata cgaccgaaca ttttccagta
    26341 acaccaggcg gtgtagagca gaatgatcgg taccagaacc accgcaaccc aggtcatgac
    26401 gttaagcgtc agctggctgg aagttgcatc ccacattgtc agacttgcgt tcatcatggt
    26461 gctggacggc atcacaaacg ggaacattgc gataccggct gtcaggatga tgcaggccag
    26521 cgtcagggag gagaacacaa acgcccacgc ggctttatcc atacgtgcag tcaggatggt
    26581 cagcagcggc agaaccacac ccagtgccgg aatagcccac agaattggcg tgttgttgaa
    26641 gttaaccagc catgcgccag cttcacgaac cacttcttta ttcagtgggt tagaggctgc
    26701 gtaatggtcc attgtcgatt tcacgacata accatcgata ccgtacatca cccatacgcc
    26761 agccagtgcg aaacagacca gtgtcaccag cgcagccacc tgagccgttg cacgggtacg
    26821 caggtgcagt tcgcccacgg tacgcatttg cagataggtt gcgccctgag taatgatcat
    26881 ccctacgctc accacgcctg ccagcaggcc gaacgggtta agcaactgga agaagttacc
    26941 ggtgtagtac agacgcagat attcatcaac gttgaacggt acgccctgca acaggttacc
    27001 gaacgctaca ccaattacca gcggcggaac gaagctacca atgaagatgc cccagtccca
    27061 catgttacgc cagcgggttt cttcaatctt ggagcggtag tcaaaaccga ccggacggaa
    27121 gaacaaagac gccagcacga ggatcatcgc cacatagaag ccggagaacg cagcggcata
    27181 gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg atcagccaaa cctggttacc
    27241 gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc tcggtgtcgt tacgaccgag
    27301 gaaacgggtg agcatgccca cccccatgtc gaaaccgtca gtgactgcaa aaccaatcag
    27361 cagaacgcca accagcagcc accagataaa acgcaatact tcataatcga tcatttgacg
    27421 actcctgtct tagcgtgccg gctgagtagt cgtggaagac tgctcaaagt gatagcgacc
    27481 ggttttcagg ctgcttgggc cgaggcgtgc aaatttgaac attaagaaca attctgccac
    27541 caggaacagg gtatacaggc cgcaaatcag caccattgag aagatgagat cgcctgcggt
    27601 cagtgacgag ttcgccacag ctgtcggcag cacttcaccg atagcccacg gttggcggcc
    27661 atattcagcc acgaaccagc ccgcttctac agcaatccac ggcagcggaa taccgtacag
    27721 cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg cggatgacac tccagaaaga
    27781 gagcgcgatg attgccagaa gcaggaagcc acacgccacc atgatacgga acgcaaagta
    27841 cagcggcgct acacgcggga tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc
    27901 atcagccacg tttggcgtat agcgtttcag cagcagaccg taaccgaggt ctttcttcat
    27961 gctattgaac tggtcacgaa ccgcctggtc ggtagaacca gaacgcagtt gttcgagcag
    28021 agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc tgcaccatca gctctttcag
    28081 gccgataacc ggggtatcca cggaacgcgt tgcaatgatg cccagtgcgt aagggatctg
    28141 aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg ccgaacagag taaaggcagc
    28201 aggcgcaggt tgcgtttccc actcggcttc aatagcagcc agtttggttt tctgcacgtc
    28261 gcccatttcg tagccggatt catcacccag aacaataaca gacagaacag cagccatacc
    28321 gaagctggca gcgatagcaa aggagcgttt agcgaaggcg aagtcacgac ctttcagcat
    28381 ataccatgcg ctgataccga ggatgaacat cgcgccagtc acataaccag acgctacagt
    28441 gtgaacgaat ttcacctgag caaccgggtt aagcaccagc tcggagaagc tcaccatctc
    28501 catacgcata gtttcaaagt tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc
    28561 aaccagaatc cacagtgcgg acaggtttga accgagcgcc accagccagg tgacacacat
    28621 atgctgaact ttacccagac gatcccaacc gaagaagaac agacctacaa aggtggattc
    28681 gaggaagaag gccatcagac cttcgattgc cagcggcgca ccgaagatat cccctacata
    28741 gtgggaatag taagaccagt tagtcccgaa ctggaactcc atggtcagac cggtagccac
    28801 acccagagcg aagttgatac caaacaactt gccccagaac ttggtcatat ctttataaat
    28861 ctgtttgccg gagaggacgt agaccgtttc cataatggcc agcaggaacg ccataccgag
    28921 cgtcagtggc acaaaaagga agtggtacat cgcggtcaag gcaaactgta agcgcgacag
    28981 ttcgactata tctaacatca tgactccttg ctcatcgcat gaagactccg agagtgaacc
    29041 ccgttagaaa gggtcacacg catgccccaa tagaaatttg tttgaacact ttcatcatac
    29101 catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca aacatgttaa taaaaactca
    29161 aattgatccc acgtatatat tacgccgcaa aatccttaca ataaacaggt ttttgttgaa
    29221 gcaaatttgc atttttcgac ggtgatcaat ttatagctaa attaccgcct ttcagccaat
    29281 ttgatcgaga acaatttatc tctttttgat gcccatttcc aagacttata cattgataaa
    29341 tatcaattcc tacaaattat gttaatcatg tttcaataag gtaactttag tgacatttat
    29401 gtttaaaatg tgtgagttat aaggctgaaa ttagtttcgg ttggcaatta aagataaaaa
    29461 gagacgaagc aaaaacagca cggttgacct taattctttt tttggtcact ggctaatacg
    29521 acgcagagtt tcagga
//
LOCUS       DAZFJF010000055        29037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFJF010000055 DAZFJF010000000
VERSION     DAZFJF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.055"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..701
                     /locus_tag="ACHKAU_002590"
     CDS             <1..701
                     /locus_tag="ACHKAU_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5531628.1"
                     /translation="EEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSWGI
                     NNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQT
                     AMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMHYFS
                     AYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTV
                     QPECPVTLKDVSDL"
     gene            698..2149
                     /gene="djlC"
                     /locus_tag="ACHKAU_002591"
     CDS             698..2149
                     /gene="djlC"
                     /locus_tag="ACHKAU_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO5531629.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2209..3879)
                     /gene="hscC"
                     /locus_tag="ACHKAU_002592"
     CDS             complement(2209..3879)
                     /gene="hscC"
                     /locus_tag="ACHKAU_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO5531630.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3963..4898)
                     /gene="rihA"
                     /locus_tag="ACHKAU_002593"
     CDS             complement(3963..4898)
                     /gene="rihA"
                     /locus_tag="ACHKAU_002593"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO5531631.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5016..5741)
                     /gene="gltL"
                     /locus_tag="ACHKAU_002594"
     CDS             complement(5016..5741)
                     /gene="gltL"
                     /locus_tag="ACHKAU_002594"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO5531632.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5741..6415)
                     /gene="gltK"
                     /locus_tag="ACHKAU_002595"
     CDS             complement(5741..6415)
                     /gene="gltK"
                     /locus_tag="ACHKAU_002595"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO5531633.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6415..7155)
                     /gene="gltJ"
                     /locus_tag="ACHKAU_002596"
     CDS             complement(6415..7155)
                     /gene="gltJ"
                     /locus_tag="ACHKAU_002596"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO5531634.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7325..8233)
                     /gene="gltI"
                     /locus_tag="ACHKAU_002597"
     CDS             complement(7325..8233)
                     /gene="gltI"
                     /locus_tag="ACHKAU_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO5531635.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8688..10565
                     /locus_tag="ACHKAU_002598"
     CDS             8688..10565
                     /locus_tag="ACHKAU_002598"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO5531636.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10643..12181)
                     /gene="lnt"
                     /locus_tag="ACHKAU_002599"
     CDS             complement(10643..12181)
                     /gene="lnt"
                     /locus_tag="ACHKAU_002599"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO5531637.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12206..13084)
                     /gene="corC"
                     /locus_tag="ACHKAU_002600"
     CDS             complement(12206..13084)
                     /gene="corC"
                     /locus_tag="ACHKAU_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO5531638.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13174..13641)
                     /gene="ybeY"
                     /locus_tag="ACHKAU_002601"
     CDS             complement(13174..13641)
                     /gene="ybeY"
                     /locus_tag="ACHKAU_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO5531639.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13638..14678)
                     /locus_tag="ACHKAU_002602"
     CDS             complement(13638..14678)
                     /locus_tag="ACHKAU_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO5531640.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14831..16255)
                     /gene="miaB"
                     /locus_tag="ACHKAU_002603"
     CDS             complement(14831..16255)
                     /gene="miaB"
                     /locus_tag="ACHKAU_002603"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO5531641.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16401..17576
                     /gene="ubiF"
                     /locus_tag="ACHKAU_002604"
     CDS             16401..17576
                     /gene="ubiF"
                     /locus_tag="ACHKAU_002604"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO5531642.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17730..17804)
                     /locus_tag="ACHKAU_002605"
     tRNA            complement(17730..17804)
                     /locus_tag="ACHKAU_002605"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17770..17772),aa:Gln,seq:ctg)
     gene            complement(17842..17916)
                     /locus_tag="ACHKAU_002606"
     tRNA            complement(17842..17916)
                     /locus_tag="ACHKAU_002606"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17882..17884),aa:Gln,seq:ctg)
     gene            complement(17965..18041)
                     /locus_tag="ACHKAU_002607"
     tRNA            complement(17965..18041)
                     /locus_tag="ACHKAU_002607"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18005..18007),aa:Met,seq:cat)
     gene            complement(18057..18131)
                     /locus_tag="ACHKAU_002608"
     tRNA            complement(18057..18131)
                     /locus_tag="ACHKAU_002608"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18097..18099),aa:Gln,seq:ttg)
     gene            complement(18166..18240)
                     /locus_tag="ACHKAU_002609"
     tRNA            complement(18166..18240)
                     /locus_tag="ACHKAU_002609"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18206..18208),aa:Gln,seq:ttg)
     gene            complement(18264..18348)
                     /locus_tag="ACHKAU_002610"
     tRNA            complement(18264..18348)
                     /locus_tag="ACHKAU_002610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18312..18314),aa:Leu,seq:tag)
     gene            complement(18357..18433)
                     /locus_tag="ACHKAU_002611"
     tRNA            complement(18357..18433)
                     /locus_tag="ACHKAU_002611"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18397..18399),aa:Met,seq:cat)
     gene            complement(18814..20478)
                     /gene="asnB"
                     /locus_tag="ACHKAU_002612"
     CDS             complement(18814..20478)
                     /gene="asnB"
                     /locus_tag="ACHKAU_002612"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO5531643.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(20735..21487)
                     /gene="nagD"
                     /locus_tag="ACHKAU_002613"
     CDS             complement(20735..21487)
                     /gene="nagD"
                     /locus_tag="ACHKAU_002613"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO5531644.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(21535..22755)
                     /gene="nagC"
                     /locus_tag="ACHKAU_002614"
                     /gene_synonym="nagR"
     CDS             complement(21535..22755)
                     /gene="nagC"
                     /locus_tag="ACHKAU_002614"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO5531645.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            complement(22764..23912)
                     /gene="nagA"
                     /locus_tag="ACHKAU_002615"
     CDS             complement(22764..23912)
                     /gene="nagA"
                     /locus_tag="ACHKAU_002615"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5531646.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            complement(23972..24772)
                     /gene="nagB"
                     /locus_tag="ACHKAU_002616"
     CDS             complement(23972..24772)
                     /gene="nagB"
                     /locus_tag="ACHKAU_002616"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5531647.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25105..27051
                     /gene="nagE"
                     /locus_tag="ACHKAU_002617"
     CDS             25105..27051
                     /gene="nagE"
                     /locus_tag="ACHKAU_002617"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO5531648.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            27253..28917
                     /gene="glnS"
                     /locus_tag="ACHKAU_002618"
     CDS             27253..28917
                     /gene="glnS"
                     /locus_tag="ACHKAU_002618"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO5531649.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
BASE COUNT         7010 a         7346 c         7550 g         7131 t
ORIGIN      
        1 aggaagaaca atatcttctt tttgcgcttt ctgcgcccat ggaaatactt aatcagggtt
       61 gcaaacccgc gcatgactcg ccaaaaatgt ataccgggat aaaagaattc gatctctcat
      121 catcctgggg aataaataat cgtgatgatt taattcagac tatttatcag atgactgatg
      181 atggtcatgc caatgattta gctggcttat atctgacatg gcatcgctct tcccctgaag
      241 agtggaaagc attaattgct ggtggctcgg agagagggtt gatttatacc caattcgttg
      301 cccaaaccgc tatgtgctgt ggagaagggg gaatcaaagc ctgggattat gttcgaatgg
      361 ggtttctgtc acgggtcggt gtgctcaaca actggctgac ggaagaagag agtttatggc
      421 tgcaatcgtg cgtctatgta cgagctcacc attactatca cagctggatg cactattttt
      481 ccgcctattc gttaggacgt ctctactggc aatcttctca gtgcgaagac aacacttcgc
      541 tacgcgaggc gctaaccctg tacaaatacg atagcgccgg gagtcggatg ttcgaagagc
      601 tggcagcggg aagcgatcgt ttctatgcca cgcttccctg gcagccatta actgtccagc
      661 ctgaatgccc ggtaacgctt aaggatgtga gcgacctatg aaaacatgtt ggcaaatcct
      721 cgaaattgaa agcacgacgc aaatagacat tatccgccag gcttatcttg ctcgcttacc
      781 gttatgtcat cccgaaaccg atccgcaggg gtttaaagca ttacgccagg cctatgaaga
      841 ggcgctgcga ctggcggtaa atcctgtcga ggaagcagat gatgaagaaa aagatgccgc
      901 cgctgaacat gaaatactgc gtgcattcag gacattactg gattcagaaa gtgatcgttt
      961 tcagccctcc gcctggcaga aatttattca gcaattaaat acctggaaca tggaggatgt
     1021 cgatcaatta cgctggccgc tgtgtgcaat cgctatagaa gcgcgatatc tttcattaaa
     1081 ttgtgcttct ttgctggcag agcgtttgaa ctggcattcg tttaatgaca gcgaaggaat
     1141 ggatgaggaa gaaagggagg cttttcttga ggccattcag gctggtgatt gtttcgattt
     1201 ccttagcctt ctggaatatc ccgttgcgtt gcagaaccag actgttgagt attacttcgc
     1261 gctggaacgt tgctgccgtt accatcctga ctacgtcact gcgtttttgg cgatggaagg
     1321 tccgtggttc attcctgatg atgcaaagtt acatcgcaaa ctgttgcgct ggtacagctc
     1381 ggtgcaaaca ggtatggcgg aactcattcc tgtagccaaa cagtggcaaa tggaagaacc
     1441 agaaagcgaa gatgcccgat attacttgtg tgctcaaagg ttgtactgcg gcgaggggga
     1501 aagcctgctt gccgatctct gcgcgtaccg ggaaagttac ccatctacac aagctgataa
     1561 tttgttgttg cagtggagca agcgtcattg cccggattat ttcgcgttat tagtgatggt
     1621 tatcgaagcg cggagcatgg tagatgcgca aggtcaaccg ctgaaatatg ttcctggtga
     1681 gagcgcccgg acgcggctgt tatgggcgga aattttacat agcggaaaat tatcgccgtt
     1741 aggtcaatcg tttattgagt cgttattctt caaacgcaaa gcatgggcgt ggtggaaatc
     1801 gagagtcggt agcgagacag aagaagattc accgttactg gatttgtatc gggtagcgga
     1861 acaggtagta cttgaagcgt ttccgaaaca agagatgctg gcccgtctta atacaaggct
     1921 ggaaggcgga gatgctcatc cattagaggc cattatcacc cggatgctgt tgacgaaagt
     1981 aaaactcgag ccggaggatg aagatgtcga tgagccaaca cctgaaaacc atgaagaaaa
     2041 aaatgatgag ggtgaaaaac cacagagcat taccagcatt atcaaaatca gtttaacggt
     2101 gctggttata ggttatgctc tcggcaaaat cgcgatgttg tttagttgac gatgtgtttt
     2161 ttgcgccgat gccggatgca gcatttacgc cgcatccggc agccgtgatt atgaggtctc
     2221 aatggctaaa taaacgctcg cccgccgcct gacctcatcg acgcgggcca tatcgtttga
     2281 ctgcaacgcc gcatcaaaat cggtgatcac ccggccaatc tcttctcgct cgtcacccag
     2341 cgcccgcgcc cacaactctt ccagtttggc cttaaaggtg cggttgatga gcatatcgcg
     2401 cggataaatt ttcaatgcgg ataaccgcgt ccgactctct tcaatctgct gcgcgctcaa
     2461 tgttaccggg ctatgattaa tgactctgga cttaacgctg ccatcttcca gaagcacgtc
     2521 aacttcaagc aagccgttga tatcataact aaagcgaata tcaatcgact gataagcccc
     2581 ggttttcttc aacggcacat cgaaggattc aaccagaatg ttatttttaa ctttgtggtt
     2641 ttctccctga tagacgttaa ccgtaattga attctgttcc gggtgcatgg tcgagtaagt
     2701 ttctacacgc gacacgggta ctgtggtgtt tcgttcaata attggcgaga aaatgccgga
     2761 aacgccctgg cggttaactt caacgcccaa cgagtaaggg caaatatcag tgaggatcac
     2821 ctcttcaata tcttcactgc gtaagcggca ggcggcctgg attgctgcgc ccagcgcgac
     2881 aatggtgctc gggtcgtaac tttgatacgg taatttgcca aacagacgca cggcgattcg
     2941 ctgcaccagc ggcatttgtg acgcgccgcc aaccagcacc agactgtcga tttgactcgg
     3001 cttcagacgt gcatcgcgca acgcctgttc aatcggcact cgcaagcgat tgagcagcgg
     3061 caaccacaaa tcttccagtt cgttctcgta aaattcgcac tcccgcgttt catcctggta
     3121 ctgccagcga atgtgcaatg gcgattgatt gctacatttt gccgcttcca cacaggcgta
     3181 caaggctccc agttcactct cgttaagcgt ggtcctggcg acatccgcgc gttttaaaac
     3241 ctcatcgacc agcatatggg taaaatcttc gccaccaaga aagttgtcgc cagcggaggc
     3301 gtgaacttca atcaccggcg tggcgtactc aagcaccgta acgtcaaacg tgccgccacc
     3361 gagatcaaac accagcgaac gggtattttg ttgggtatgc aggccatacg ccatcgcagc
     3421 tgctgtgggt tcattaatta agcgtaccgc atttaacccg gctaactccg ctgctaaacg
     3481 ggtatgcttg cgttgttcat cgctgaaata agccggaacg gagatcacca catctttaat
     3541 cggacgttgc agaaattctt cggcatcttc ttttaatgag cgtaatacca aagaggacag
     3601 ttctggcgcg ttaaatgtgt cgctgcctaa ccgccagttg gtattactgc ccattgcacg
     3661 tttaaataac gctgccgttt tatccggatg cgaagtacgc cgtgatacag ccggttttcc
     3721 gactaaaata tgattatttt catccatgct aattatggat ggtgttaaat attcaccgaa
     3781 cttatttgga attaattgcg cggcaccgtc tttccagacg gcaattaaac tattggtagt
     3841 gccgagatcg ataccaatgg cgagttctgc attatccatt gcatttatat cttgaaagaa
     3901 aaagagataa acagattaag accacccagg taacgcgtgg tgatcttaat caatgacgtg
     3961 tgttaagcgt aaaatttcag acgatcagcc agtaaatcaa caaagccctg acgatcaaca
     4021 tcgaccatta cagtggcatt cggtttattg ccagtcagat aataataatc aacaaccgtc
     4081 ataccctggc tatatttccc ctgtgtttcc acgccaaccc agcgctcaac agtggtaaac
     4141 aactcgggtt tcaacagcca ggcgatggtg catgggtcat gcagcggtgc accgacaaag
     4201 ccccattttt cgtctttatg atattcgagg aagaaatcca gcagttcggc aacaatggtt
     4261 gaaacagggt tgccaatcgc gcggaaacgc tcggtgtctt caacgtggat ttgtgctttg
     4321 tgagtaacat ccagaccggc catcaccact gggatccctg actggaagac aatttctgcc
     4381 gcttccgggt caacgtaaat attaaattca gccgcaggcg tccagttacc aagccccatt
     4441 gcgccgccca taatcacgat acgggcaatt ttgctatgca gttccggatg gctattgagc
     4501 agcaaggcaa cgttagtttg cggtccggta gacacaatcg tgacaggttc cgcactttcg
     4561 cgcagcgttt tcgccatcag ctctaccgcc gtacagtttt gcggtgcgaa cgtcggttcc
     4621 ggtaatgccg ggccatccag accgctttcg ccgtgcacat tgtccgcgat aatcaactca
     4681 cgcattaacg gttttaccgc gccgcctgct accggaatat cggtgcgatt aagcaaggtc
     4741 agcatacgca gaacattgcg taaggttttt tctggtgtct ggtttccggc ggaagacgta
     4801 attgctttga catcaagctc tggtgaggcg agggcgagaa ctattgcgat agcgtcgtca
     4861 tgacctgggt cgcaatctaa cagaattggc agtgccattg ttgctccttg ttgtgtgctt
     4921 ctttgcgaca agggtaacgc caggatgtaa cagatacgag gggcgaaacg ataaagcgtg
     4981 agatggcgcg cacttgggta tgcgcgccag agtgattaat gcaggatttt cgcgaggaag
     5041 tctttcgcgc ggtccgattt cggatcatcg aagaaagcgt ctttcggcga gtcttcgaca
     5101 attttaccct cgtccataaa gatcacccga ttcgccactt tgcgggcaaa gcccatttcg
     5161 tgggtcacca ccatcatggt cattccttcg ttcgccagtt ccaccatcac gtccagcact
     5221 tcgttgatca tctccggatc cagcgccgat gtcggttcgt cgaacagcat cgcaatagga
     5281 tccatacaca acgcgcgagc gattgccaca cgctgctgct gaccgccgga aagctgcgcc
     5341 ggaaatttat tggcatgtgc ggaaagtccc acgcgctcca gcaatttcaa tgctttttca
     5401 cgagcgggcg ctttatcgcg tttaagcact ttcacctgcg ccagagtcag gttttcaata
     5461 atcgacaaat gggggaacaa ctcaaaatgc tggaacacca tcccgacgcg ggaacgcagc
     5521 tttgccagat cggttttctt gtcattaacc acgataccat cgacggtgat ttcaccttgc
     5581 tgcactggtt cgaggccgtt gacggtttta atcagcgttg atttgccgga accagacggg
     5641 ccgcaaacca ccaccacttc gccttttttc acttcggttg agcagtcggt cagcacctga
     5701 aagtgaccat accattttga aacatttttc agggtaatca ttatgctgtc cttcttttca
     5761 agtagctgac caacaacgac gcgctaagac taataacgaa ataaacaaat ccggcaaata
     5821 ggatcatctc aacctgcgta ccatcacgct caccaatggt tgaggcggta cggaagaaat
     5881 cggccagaga taacacatac accagtgagg tatcctggaa cagtacgatg ccctgagtga
     5941 gcagaagcgg caccatcgcg cggaacgcct gcggcagaat aatcagtttc atcgactgcc
     6001 agtgagtcat tcccaacgcc agcgcggcgc tcgactgacc acgagaaata ctttgaatac
     6061 cagcacggat aatctctgaa tagtaggccg cttcaaacat tgaaaacgcc accatcgccg
     6121 aaattaaacg gatatcattt tttggtgata atcccagcac gttttgcaga aaacctggca
     6181 cgatcaggta aaaccacagc aaaaccataa ctaaaggaat cgagcggaat acgttaacgt
     6241 aggctttggc aaaccacgct acgggcgcaa agctggacaa gcgcatcacc gccagcatgg
     6301 tgccccacaa aataccaatc acgactgccg tgacggtgat tttcagcgtg atcaccagcc
     6361 cgtcgagcag atatggcagg gaagggacaa tggaactcca gtcaaactcg tacattattt
     6421 gccccccatg ttgccaggca ggcgaacttt acgttcaacc agcgtcatta ccagcatgat
     6481 aaccgcgtta atcaacacat acgccagcgt aatagcggta aacgactccc aggcgtgggc
     6541 tgagtaatca agcaatttac ccgcctgcgc cgccatatcc accagaccaa tagtcgaggc
     6601 gatggcggag tttttcacca ggttcatcat ctcggaagtc atcggcggga cgataacgcg
     6661 ataagcatta ggcagcagta cgtatcgata agcctgcggt agcgtcaggc ccatcgccag
     6721 cgcggcattt ttttgcccac gcggcaacga ctgaatggcg gcgcgtacct gttcgcaaac
     6781 acgggcggcg gtaaacagcc ccaggcagag catggatgaa aggaaaaact gaatattggg
     6841 atccagctcg gccttaaacc acataccgat tttctctggc agcaactccg ggatcaccag
     6901 ataccaggta aagaattgca caatcaacgg cacgttgcgg aacagttcga catacaacgt
     6961 acccagaccg gagagaaaac ggtttggaac ggtacgtaaa atgccgaaaa atgaaccgac
     7021 gaggaaagcg ataatccagg cgcagatcga caaagcgatc gtcacctgaa aaccactcca
     7081 gatccaacca agataggtgg tgttgccgaa cggggcttgt tgtaaaaaaa taccccagtt
     7141 ccagtctata gacataaatc tactccagaa aaaagagggt agcagcgtta actgctaccc
     7201 tcgaagattg ttacccagcg tattgcggtt ttcaggcccg atggggaacg accatcaggc
     7261 gtatagtctg tccgtgctac gtaacaatcg agagggctgg aatttccgcc cctggttctt
     7321 gtaattagtt cagtgctttg tcattcggtt ctttgaacag tgctttcatt tcgtctgaca
     7381 gttcgaaatt catgttcagg tttttcggcg gaattggatt tttgaaccat ttatcaaacc
     7441 atttttccgc ttcaccggaa gtctgcacct gagcgatagt gtcatccatc agctttttga
     7501 actgcggatc atctttacgc aacatgcaac cgtaggcttc ctgagactgc ggcttgccga
     7561 cgatatccca gttgtctggt ttcttcgctt tcgcacgttc accggccagc agggcatcat
     7621 ccatcataaa ggcaacggca cgaccgcttt ccagggtacg gaaagagtca ccgtgatctt
     7681 tggcgctaat gatgcgcata ttcatttttt gctcttcatt cagtttgttg agcaaaactt
     7741 cagaagtagt gccggaagtg acgactacgg ctttgccttt caggtcggca aaatctttga
     7801 tatcgccgcc ctttttggtc agcaggcgcg taccgaccac gaaaatagta tcagagaaag
     7861 ccgcctgttt ttggcgttcg acgttgttgg tggtagaacc acattcaaaa tcgaaagtgc
     7921 cgttttgcag cagtggaata cggttttgtg aggtaatcgg aatcagtttt acctgcaagt
     7981 ccggtttgtt gagtttcttt ttcactgctt caacaatggc gttggagtaa tcctgcgagt
     8041 aacccaccac tttttgctga ttgtcgtaat aagagaaagg tactgaagat tcacggtgac
     8101 cgacgacaat cacgccgttt ttggcgattt tgtccagcgt actgcccgct accggggcgg
     8161 cgtcatctgc ctgtgccagt cctgcggaaa gcgccagggc gaggattgct gtggcaggtt
     8221 tacgtaattg catatccaac tcctttatct tctgcgttaa gaacgcatgg atacccgttg
     8281 tgagtgtttg tgttgttatc gtctgcaact ttattgtgca gtgttgtgcc tgttaggtaa
     8341 catttagttt ggctaaatgt aaagatattg ctgttttatt gtttgttttt gcgagatgcg
     8401 ccgcaccatt ccgaagcaaa attcttaaac cgcacctttt tggtgctacc gctgatttgt
     8461 ggtggtgcaa cctggttgcg tcgcggctga tttcatcgct tacacagtaa ataaagcaac
     8521 ttacatgcca aaaaatactt aggggcgaat taattacgat tgtaatttta ggctgaaaaa
     8581 gtcatatttc ggacagaatt atcttttcgg catgggacgt ttgccgggat gataactgca
     8641 cacgggaaaa atgcttttta cctgtctttt caaggaagaa acaccttatg tctgcatctt
     8701 cggttaagcc gttaaatgtt caattacccg caataaccct tatccttttt gcactctgta
     8761 ttgggatatt ttgttatctc gcacaatgga tgagttatga agaggtcgat caatccgcac
     8821 ttatccatct cggtgcaaac gttgcgcctc ttactttgtc gggtgaaccc tggcgcttat
     8881 tgagcagtat ctttctgcac agtagtgttt ctcatctgct gatgaatatg tttgcattcc
     8941 tggtcgtggg aggcgtggcg gaacagattc tggggaaatg gcgactcctg attacctggt
     9001 tattctccgg cgtctttggt gggctcatca gcgcctgtta tgcgttacgc gaaagtgagc
     9061 agatagtcat cagcgttggg gcatccgggg cgattctggg aatagctggt gcagcgatag
     9121 caacacagtt tgcttcaggt acgggcacat accataaaaa ccagcggcga gtatttcctc
     9181 tgttgggtat ggtggcgctg acactgttgt acggtgcccg gcaaacagga atagataacg
     9241 cttgccacat tggcggcctg attgcgggtg gcgcgttggg ttggctgagc gcgcgtttat
     9301 ctgggcaaaa cagactcgtt acggaaggcg ggattattgt tgcgggcagt cttcttctga
     9361 ccggggctat ctggcttgcg cagcagcaga tggatgagtc agttttacag gtcaggcaaa
     9421 gcctgcgtga agcgttttat ccgcaggaga ttgaacaaga gcgacgacaa aaaaaacaac
     9481 agttagcgga ggaacgcaac gccctcaggg aaacattatc cgctccggta agtcgtgaac
     9541 aggccagtgg tgatttactc gctgagattg ccgatatcca tgatatggcg atcagtcggg
     9601 atggtaatac gttgtatgcc gcaattgaaa acaccaacag cattgttgtt ttcgacctcg
     9661 gacaaaagaa aatcctgcat acctttacag cgcccatagc gaaagaaaag tcagtcaaac
     9721 attgtggtgg ctgtaaagat cagggcgtca gatcgctggc attaagcccg gatgaaaagt
     9781 tgatttatgc gacgtcattt gaagcgaatg cgttatcggt cattaacgtg gcgacagggg
     9841 agattattca gtcaattacc accggtgcac atcctgacag ttttatcctc tcgcgtgatg
     9901 gcacaaaagc ctgggtgatg aatcgcacca gtaatagtgt gtcagcgatt gatctggtga
     9961 cttatcagca tgtggcggat atcccgctgg agaaatacga cgggacgggg acgagcggta
    10021 aaccaggcgc ctgggttatg gcactttccc cggatgaaag aacattactg gttccgggag
    10081 caggcagagg taacatcgtg cggatcaata ccatcacgca tcagaaagaa gactttcccg
    10141 caggtgatgc gcgtggaacg atatcggcga tgcgttttcg acctgaaaac ggcgaggtta
    10201 tttttgccga tagtcagggg atttcacgta taagcgtagg ggatcaacaa gccagcatta
    10261 tgacgcaatg gtgtagcagg agcgtttatt ccgttgaggg tattagcccg gacggtcaat
    10321 atttagcgtt ggtgtcatat ggcctgcaag gttatgtcat cctgctcaat attaatgccg
    10381 ggcagattat tggcgtttat cctgccagct acgttaatca ccttcgtttt tcagcggatg
    10441 gtagaaaaat atttgtcatg gcgaagaacg ggttgatcca aatggacagg acgcgctcgc
    10501 ttgatccgca ggcaattatt cgtcatcccc aatatggcaa tgtggcttgt atccctgaac
    10561 cgtaagaaaa aggggcatga taaccatgcc ccaaagctca atgagaaagt acgttttgct
    10621 catgcaggat acatcaggca catcaacgtt tacgtgctcg cagactcatc agcacagcgg
    10681 caaaaccaaa cagcgccgtc agcacccaca gcggccagtt gccggtacgt gcatacggtg
    10741 tgagtccggt ggtcggtgtc acattagtgg tcaacacctc gcgggtgaac tgcgggatca
    10801 tcgcctgaat ctcaccctgc gggccaatca ccgccgtaat gccgttgttg gtgctgcgca
    10861 acagtgggcg cgccagctcc agcgcacgca ttcgcgccat ctggaagtgc tgccatggac
    10921 caatagactt accaaaccac gcatcgttgg agatagtcag cagatagtcg gtatccgggc
    10981 ggaagttatc gcgcacttgc tcgccaagaa tgatctcgta gcaaatggcc gcagtaagct
    11041 caataccatt taccgacagc ggcggctgga tatatggccc acggctgaac gacgacatcg
    11101 gcagatcaaa gaacggtgct aacggacgca gaatcgactc cagcgggaca aactcgccaa
    11161 acggcaccag atggtttttg ttatagcgat cggctgattc gtagctgtac ggtgcacctt
    11221 tacccagcgt gatgatggtg ttgtaggtat cgtagcggtt ctgcttattg agacgcgcgt
    11281 cgacaatccc ggttaccagc gagctacctt tatcacgcaa ctcaccgtcc agtgctttga
    11341 ggaacggttg ctgattaatt tccagatcgg ttatcgccga ctccggccag ataatcaacg
    11401 atgatttgcc cagcagcggt gccgttgcgt tgtagtaaat cttcagcgta ttaagaagct
    11461 ggtctccgtc ccatttcagt gattgcggaa tatcgccctg aaccatcgaa acctgaatgg
    11521 ttttctccgg ttgcggggta aaccattgga tgtaacgcag cgggaaggga agggcaaaca
    11581 gcacgacggc caccaccagc ggacgccagt tgcgtttgac taacgccagt gccagcaggc
    11641 cgctaaccat catcagcagg aagttaatgg cttccacgcc cattattggt gccagccctt
    11701 ttaacgggcc atcaatctgg ctatagccga actgtaacca cgggaagcca gtcagcaccc
    11761 aaccgcgcag aaactcggtc acttgccaga gagcaggggc ggcaatcgcc acgcgcagcc
    11821 aggtggtttt cggccacagg cgcgacagca cgccagcaaa cagtccggta tacagcgaca
    11881 aatacgccgc cagcagcacc accaggaaga tgttaaccgg gccaggcatc ccgccaaagg
    11941 tcgcgatgct gacatagacc cagttaatac cggtgccaaa gaggccaaat ccccagcaga
    12001 agccaatagc ggcagactgg agcgggcggc ggttaaaggt cagcgcctga agccccatca
    12061 gcgaaacaat cgccgcaggc cagacatcgt aaggagagaa ggccagcgtt ccgcaggcac
    12121 cgaataataa cgccagcagc aggcgaatgc gctggcgttc aattaatgag gcaaaagcca
    12181 tgtagttatc tatccagttt cggttttatt catccagctt tggctggggt gagtcatccg
    12241 ggattttgac atgaacctga ataatacgcc gactatcggc catcgccact ttgaactggt
    12301 aaccgtcgat gtcgatagtt tcgccacgcg ccggaagatg cccaaacgcc tgcatcacca
    12361 gaccaccgat agtgtcgact tcttcatcgc taaagtgggt gccgaacgct tcgttgaagt
    12421 cttcaatgga agccagtgcg cgcacggtcc aggtatgacg actcagctga cggaagtcga
    12481 tatcatcttc ttcgtcatac tcgtcttcaa tctcaccaac aatcagttcc aggatgtctt
    12541 caatggtcac cagaccggaa accccaccga attcgtcaat aacgatcgcc atgtggtaac
    12601 gctgagagcg aaactctttc agcatccggt ctacgcgctt actttcagga acgacaaccg
    12661 cctgacgtaa cactttgtcc atgctgaagg cttcagcatc gctgcgcata aacggtagca
    12721 agtctttcgc catcagaatc ccttcaatgt gatctttgtc ttcgctaatc accgggaaac
    12781 gtgagtgggc ggactcgatg atgacatcaa ggcattcgtc cagcgtctgg ttgcgtttca
    12841 gggtaatcat ctgggagcgg gggatcatga tgtcgcgaac gcgttggtct gcgatgtcca
    12901 tcaccccttc gagcatatcg cgcgtatctt cgtcgataag gtcgttctgc ccggaatcac
    12961 ggatcagcgc cagcagttcg tcacggtttt tcggttcacc gtggaaaagt tggctgagta
    13021 acagggagaa aaatcccttc ttgttgctta tcgtgtcact actgtgtgaa ttgtcgtcgc
    13081 tcatggcgtc gtatgggttc tcatgttagt taatcaaaac gccgtcgtta atcaccaacg
    13141 gcggggacgt ctgccagtca aatgcctggc aaattattct ttctcggcaa tgtacggatc
    13201 ctcatagccc agagcaagca taatctctgt ttcgagggct tccatttctt ctgcttcgtc
    13261 atcttcgatg tgatcgtaac ctaacaaatg cagactgccg tgcaccacca tatgcgccca
    13321 gtgcgcctcc agtggtttgc cttgctcctg agcttccttc tcaaccacct gacggcagat
    13381 aaccagatcg cccagtagcg acatttccat gccaggcggc acttcaaacg ggaaggagag
    13441 cacgttggtc ggcttatcct taccgcgata ggtcagattc agactgtggc tttcggcggt
    13501 atcgaccacg cgaatagtca cttccgattc ttcctgaaac tgcgggatca ccgcattcag
    13561 ccatgtttga aactggctct cttccggtaa cccggaatta tcttcacatg ccagttgtaa
    13621 atcgaggatc acctgactca tttttgttcc tgttcttcgc gcttgcgttc tgccgccagc
    13681 gccgcttttc gtttttgttc ggcttcttcc caggcttcat aggcgttaac gatacgcgcc
    13741 accaccgggt gacgaaccac gtcttcgctg tggaagaagt taaagctgat ctcttcgaca
    13801 tcggccagca cttcgatggc gtgacgtaag cctgatttag tattacgcgg caggtcgatc
    13861 tgtgtgacgt cgccggtgat aaccgctttt gagttaaaac cgatacgggt caggaacatc
    13921 ttcatctgtt cgatggtggt gttctggctc tcatcgagaa tgataaacgc gtcgttcagc
    13981 gtacgaccac gcatataggc cagcggcgcg acttcaataa cgttgcgctc aatcagtttc
    14041 tcaactttct caaagcccag catttcaaac agcgcgtcgt acagcgggcg cagatacggg
    14101 tctactttct ggcttaaatc gccaggcagg aagcccagtt tctcaccggc ttcgaccgcc
    14161 ggacgagtca gcagaatacg gcgaatctcc tgacgctcca gggcatcaac tgccgcagcc
    14221 actgccaggt aggttttacc cgtacccgcc gggccaacgc cgaaggtaat gtcatggtcg
    14281 agaatattgg cgatgtactg cgcctggttt ggcgtgcgcg gcttaattac gccgcgtttg
    14341 gttttgatat tgaccgcttt gccgtactcc ggcacgctct ccgcactttg ctccagtact
    14401 cgcgcttctt taatcgcaag gtggatctgt tccggttcga tatcctgaat ctgaccgcgc
    14461 atcggggctg tatcgacata caggctacgc agaatgtctg ccgcagcggt gacgcaaatc
    14521 ggacggccgg tcagtttaaa gtggttgtcg cggcgattga tctcgatgcc gagacggcgt
    14581 tcgagctgct tgatgttgtc atcaaacggg ccgcacaggc tcaacagacg cgcgttgtct
    14641 gctggctcca gggtgatttc gcgagtgtct atgttcaaac cgttcctctt atctgtatgt
    14701 cgccggaagc tgaacattca ccggcctata aggaaattat tcacgccaca ggaaaaaggc
    14761 gcaagcattg cgataaagat ggggataaag agagaaaaaa caaggcccac cggaacggcg
    14821 ggcctgagaa ttacggctga taataaccca cgccaaggtc gttttctttg cgggtacggg
    14881 caatcaccga ttccggtgtt tctgccacgc gcagacccat ttcatcttca gtacgcacca
    14941 ctttaccgcg cagagagttc gggtagacgt cggtaatttc tacatcgacg aatttaccga
    15001 tcatatccgg tgtgccctcg aagttgacca cgcggttatt ttcggtacgc ccggaaagct
    15061 ccatgatgct cttacgcgaa gtcccttcta ccagaatacg ctgggtggtg ccgagcatcc
    15121 ggcggctcca tgccatcgct tgctgattga tacgttcttg cagaatatac agacgttgct
    15181 tcttctcttc ttccggaaca tcatcaacca tatcggcggc tggtgtaccc ggacgtgcag
    15241 agaagataaa gctgtagctc atgtcgaaat tgacgtcggc aatcagcttc atcgttttct
    15301 cgaagtcttc ggtggtttcg ccagggaagc caacaatgaa gtcagaactg atctgaatat
    15361 ccggacgcgc cgcacgcagt ttacggatga tcgctttgta ctccagtgcc gtatgagtac
    15421 ggcccatcag gttcagaatg cgatcggaac cgctctgtac cggcagatgc aggaagctca
    15481 ccagctccgg cgtgtcgcga tacacttcga tgatatcgtc ggtgaattcg atcggatggc
    15541 tggtggtaaa gcgaatacga tcgatcccgt cgatcgcagc aaccagacgc aacagatcgg
    15601 caaacgatcc ggtggtgccg tcgtagttct caccacgcca ggcattcaca ttctgaccga
    15661 gcaggttgac ttcacgcacg ccctgagccg caagctgggc gatctcaaac agaatatcgt
    15721 cggacggacg gcttacctct tcaccacggg tgtaaggcac cacgcagtag gtgcaatatt
    15781 tattgcagcc ttccatgatg gagacaaacg cggtcggccc ttcggcgcgc ggttccggca
    15841 gacggtcaaa cttctcgatt tccgggaagc tgatatctac aaccgggctg cggtcgccgc
    15901 gcacggagtt gatcatctcc ggcagacggt gcagcgtttg cggcccaaaa ataatatcga
    15961 catagtgggc gcgctggcga atgtgctcgc cttcttgcga tgccacgcag ccaccgacgc
    16021 cgataatcag gtctggattc ttctctttta acagtttcca gcgacccaac tgatggaaga
    16081 ctttttcctg agccttctcg cggattgagc aggtgttcag cagcagcaca tccgcttctt
    16141 ccgccacgtc ggtcagttga tagccgtggg tggcatccag cagatcggcc atcttcgatg
    16201 aatcgtactc gttcatctga cagccccagg ttttaatatg gagttttttg gtcatcgact
    16261 tgctcttgcg aaatagtagc caggaatgca gggcgcatag tgtaatgctt tgctgccgtt
    16321 gtgaccagta tgagcgttat cagcccttgg gggtaaaaat cctgtaaact taaagcatat
    16381 tgctaacagg atgattgacc atgacaaatc aaccaacgga aattgccatt gtcggcggag
    16441 gaatggtcgg cggcgcactg gcgctggggc tggcacagca cggattttcg gtaacggtga
    16501 tcgagcacgc agaacctgca ccgtttgtcg ctgatagcca accggacgtg cggatctcgg
    16561 cgatcagcgc cgcatcggta tcattgctta aagggttagg ggtctgggat gcagtacagg
    16621 ctatgcgttg ccatccttac cgcagactgg aaacgtggga gtgggaaacg gcgcatgtga
    16681 tgtttgacgc cgctgaactt aagctgccgc tgcttggcta tatggtggaa aacaccgtcc
    16741 tgcaacaggc attgtggcag gcgctggaag cgcatccgaa agtaacgtta cgtgtgccag
    16801 gctcgctgat tgcattacat cgagataatg atcttcagga gctggaactg aagggcggtg
    16861 aagtgattcg cgcgaagctg gtgattggtg ctgacggcgc aaattcgcag gtgcggcaga
    16921 tggcgggaat tggcgttcat gcctggcagt atgcgcagtc gtgtatgttg attagcgtac
    16981 agtgtgagaa cgatcccggc gacagcacct ggcagcaatt taccccggac ggaccgcgtg
    17041 cgtttctgcc gttgtttgat aactgggcat cgctggtgtg gtatgactct ccggtacgta
    17101 ttcgccagtt gcagaatatg aatatggcgc aactccaggc ggaaatcgcg aagcatttcc
    17161 cgtcgcgtct gggttacgtt acaccgcttg ccgctggtgc gtttccgctg acgcgccgac
    17221 atgcgttgca gtatgtgcag ccggggcttg cgctggtggg cgatgccgcg cataccatcc
    17281 atccgctggc ggggcagggg gggaatcttg gttatcgtga tgtcgatgcc ctgattgatg
    17341 ttctggtgaa tgcccgcagc tacggtgaag cgtgggccag ttatcctgtc ctcaagcgtt
    17401 accagatgcg gcgcatggcg gataacttca ttatgcaaag tggtatggat ctgttttatg
    17461 ccggattcag taataatctg ccgccgctgc gttttgtgcg taatctcggg ttaatggcgg
    17521 cagagcgtgc tggcgtgttg aaacgtcagg cgctgaaata tgcgttaggg ttgtagcctt
    17581 acaacattgc cggatgcgtg ccaaccgtag gtcggataag acgcggcagc gtcgcatccg
    17641 acattgaagg ataagacgtg tcaacatcgc attcgacatt gaatgaacgc agaaaagcaa
    17701 aaagctcgcc gaagcgagct tttttaatgt ggctggggta cgaggattcg aacctcggaa
    17761 tgccggaatc agaatccggt gccttaccgc ttggcgatac cccaactggg cgcacttacg
    17821 atgtaagcgt cttgaataaa ttggctgggg tacgaggatt cgaacctcgg aatgccggaa
    17881 tcagaatccg gtgccttacc gcttggcgat accccaacaa attggttttt gaatttgccg
    17941 aacgtattcg acacattcag aatttggtgg ctacgacggg attcgaacct gtgaccccat
    18001 cattatgagt gatgtgctct aaccaactga gctacgtagc cagattgttt cttcgatggc
    18061 tggggtacct ggattcgaac cagggaatgc cggtatcaaa aaccggtgcc ttaccgcttg
    18121 gcgatacccc aataaccggg cggtgaaccg cttactcgaa gaagatggct ggggtacctg
    18181 gattcgaacc agggaatgcc ggtatcaaaa accggtgcct taccgcttgg cgatacccca
    18241 tccgtacaac gctttctggt gaatggtgcg ggaggcgaga cttgaactcg cacaccttgc
    18301 ggcgccagaa cctaaatctg gtgcgtctac caatttcgcc actcccgcaa aaaagatggt
    18361 ggctacgacg ggattcgaac ctgtgacccc atcattatga gtgatgtgct ctaaccaact
    18421 gagctacgta gccatctttt ttttcgcgat accttatcgg cgttgcgggg cgcattatgc
    18481 gtatagagcc ttgcagcgtc aacctctttt tcaaggaaaa ttgctcgaaa gtgactgttt
    18541 ggttaggttg cgaacagcgt ggcgctatat tcgtcaatta ttgtttactt tgtgtttttt
    18601 ccccacccta cagccattct tttgtcgtac aggatgaaat tcggaattca acaatagtgg
    18661 tgggaaatta atctatgaaa tactggccta cagtgatgag ttgtcaaaca gtgatgtggc
    18721 aaacccggaa catttcctta ctgcatatca gaattaacaa gcacctcaat aactgaaaca
    18781 gccccggatt tcaccggggc tgtttcgcat ttcttactta tacgccgact ggtgaacgcc
    18841 aaccgcacga ccagacggat cgtccatttt cttgaacgcc tcatcccact caatcgcttt
    18901 agcggaagaa cacgcgacgg aaggaccgcc aggcacgcac tcagcggcgc tcggaagcgg
    18961 gaacagttct tcaaagatct cccggtacag atacgcttct tttgaggttg gcgtgttgta
    19021 cgggaagcgg aagcgggcag tttccagttg ctgatcagaa acctgctgcg ccgccacttc
    19081 tttcagggtg tcgatccaac tgtaaccgac gccatcggag aactgctctt tctgccgcca
    19141 ggccacgctt gcgggcaggt atgactcaaa acattcacgc aggatgtgtt tttccatttt
    19201 gccgttaccg cacattttat cctgcgggtt aatgcgcatc gccacatcaa ggaatttttt
    19261 gtcgaggaac ggaacgcgtg cttccacgcc ccaggctgac atcgctttgt tggcgcgcgc
    19321 gcagtcatac atatgcaggg ccagcagttt acgcaccgtc tcttcatgca gttctttggc
    19381 attcggtgct ttgtggaagt aaagataacc gccaaacact tcatcagaac cttcaccgga
    19441 cagcaccatt ttaatgccca ttgccttgat cttacgcgac attaaataca tcggtgttga
    19501 agcgcgaata gttgtcacat cataggtttc gatgtggtaa atcacgtcgc ggatggcatc
    19561 cagaccttcc tgtacagtga agtgaatttc gtgatgcacc gtgcccagat ggtttgccac
    19621 ttcctgggct gctttcagat ccggtgaacc cggcagacct acagcaaagg agtgtaattg
    19681 cggccaccag gcttcagagc gttcctgatc ttccacgcga cgggcggcgt atttcttggt
    19741 gatcgcggaa ataattgagg aatccagacc acccgagagc agcacaccgt aaggaacgtc
    19801 agacatcaga tggcttttaa cggaatcttc cagtgcctga cgcagctcgt ttttgtcggt
    19861 cacgttatct ttcactgcat caaagtcgaa ccagtcgcga tgataataag aacggatttc
    19921 gccgtcctga ctccacaaat agctccccgc cgggaactct ttaatcgtgc ggcaaactgg
    19981 caccagggct ttcatttctg aggccacata cagctgaccg tgctcgtcat agcccatata
    20041 cagtgggatg atccccagat ggtcgcgacc aatcagatat gcatcttttt cgctgtcgta
    20101 cagggcaaag gcaaacatgc cctgcaagtc gtcgagaaat tccggccctt tttcctgata
    20161 cagcgcgagg atcacttcgc agtcagatcc ggtctggaac tgataacgat cgccatattc
    20221 ggcgcgcagt gcctggtggt tgtagatttc accgtttacc gccagcacat gagttttttg
    20281 ttggttgtag agaggttgcg cccccgcgtt aacgtcaaca attgacagac gttcgtgggc
    20341 gagaatggcg ttatcgctgg cataaatacc ggaccagtcc gggccacgat gacgcatcag
    20401 gcgtgacagc tcgagtgctt tcttacgcag ctcaactgcg tctgttttga tatcgaatac
    20461 gccaaaaatt gaacacataa ccttctccgt taacctggta tttgttgctt gttgtgtttg
    20521 cttgtttaaa aaaatgccgc aaagcagcac cgtgcgcaag cgatttggca gtgcaaaaat
    20581 aaaaaacgta atggtgattg ctgatttgtg aaaaagggga gggtgtggtg atgtatttat
    20641 tgatggatcg ataatttttg gcgagtttta ttgaatggga tgattttaat gatgggtaaa
    20701 aataagaaac cacgccctga gacgtggttg ctattcagat aacgtcgatt tcagcgaccg
    20761 acgggtaaat ccagcttggg cggaacggca tactgtcgat atcgtcgagc gatgaaacac
    20821 cagaaagcac cagaatcgtc tccagacccg cctggaagcc cgccagaatg tcggtgcgca
    20881 agttatcgcc gacaatcacc gtttcttccg aatgcgcctg cattttgttt aatgccgcgc
    20941 ggatgatcca cgggctgggc ttaccaacat agaacggttt gcgcccggag attttctcaa
    21001 tccctgcaca caacgcgcca caagcgggat aaaaaccgcg cccgtgggtg tccgggttgg
    21061 tggcgataaa gcgtgcaccg ttagcgacga aataggccgc tttatgcatc atgtcccagt
    21121 tgtaggaacg cgtttcgcca acaatcacaa aatcagggtt cacatcggta atagtgaaac
    21181 cggctttgta cagttcatga atcagtgcgc cttcgcccac cacatacgct ttcttgcctt
    21241 cctggcgacg caggaaatcg gcagtcgcca tcgcagaggt ataaaacacg ctatccggca
    21301 catcgacacc ggcagtggca aagcggttcg ccagatcttg cccggtctgc gaaggatagt
    21361 tggtcagcaa caccagcggc aggcctttat ccataatccc gtgcaaaaat tccgctgcac
    21421 ccggtacggc gacgttatcg tgcatcagca cgccgtcgat atcgcaaatt acatttttaa
    21481 tggtcatgga ctacccagaa tattgacaac aataagcgcc actataaaag cacattaatt
    21541 ttccagcaaa tgctggagca aaataccgtt gagcatggcg cgttttacca gcgcaaaagc
    21601 gccgattgcc gagcggtgat ccagctcaga acgtaccacc ggcagattag tgcgaaacgc
    21661 cttcagcgcc tgggtattaa tgcagctttc aatagcaggg agcagcactt tatcggcttc
    21721 ggtgatttca ccggcaataa caattttttg cggattaaat aagttgatag caatggcgat
    21781 ggttttgccc agatgacgac cgacatactc aatcacttcc gaggccaggc tatcgccttt
    21841 gttcgcggct ttgcagatag ttttgatggt gcagtcgtcc agcggcacgc ggctctggta
    21901 gccctgcttt aacagattca acacccgttg ttcaatagcg gcgttggcag cgatagtttc
    21961 caggcagcca aagttgccgc agtggcagcg ttcacccagc ggttcgacct gaatatggcc
    22021 aatttcaccg acgttgccgt tgcggccaat aaaaatgcgc ccgttagaga taatcccggc
    22081 cccggttccg cgatggacac gcaccagaat ggagtcttcg caatcctgac ttgcaccgaa
    22141 gtagtgctcc gccagcgcca gactacggat atcgtgacca acgaaacagg tcactttaaa
    22201 acgttcttcc agtgcttcta ccagccccca gttttctacc tgaatatgcg gcatgtaatg
    22261 aattttgccg ctgtccgggt caacaagccc tggcaggatc accgaaatcg cgatcagctc
    22321 gcgcagtttg cgctggtagc tatcaataaa ctgagcaatg gcattcaaca gggcatgttc
    22381 cagcgtttgc tgggtacgtt ccggcagcgg gtaatgttct tctgccagca ctttgctgct
    22441 gagatcaaac agggtgatgg tggcgtcgtg acgaccaagc cgtacgccga ttgcgtggaa
    22501 attgcgggtt tcggtgacga tggagatagc gcggcggccc ccggtggagg cctgctgatc
    22561 aacttctttg atcagcccgc gttcgataag ctgacgcgta attttggtta cgctggcggg
    22621 ggcaagctgg ctttgctcgg caatctgaat ccgcgagatt ggcccgtact ggtcaatcag
    22681 gcgataaacc gccgcgctgt taagctgttt tacgagatca acattaccta tctgagcttg
    22741 tccgcctggt gtcatacttt ctcttattga gttacgacct cgttaccgtt aacgatggtc
    22801 ttggtgattt taaaatcagg tgtgaatgcg gtcaggttgg ctactttacc tgcggcgagt
    22861 gtgccgagac gtttctcaac gccaatcgca cgcgccggat agagcgtcgc catacgcagc
    22921 acttcatcca gtgcgatacc gcaatgttcg accagattac gcacgccttc aatcatggtt
    22981 aaggatgaac cgcttaacgt accgttctca tccacacaaa gtccgttacg gtagtatatt
    23041 gttttacccg caaaaatgaa ctgttcaatg ttggcacctg ctggcgcggt ggcgtcggta
    23101 accagacaca gtttgtcgcc tttcagacgt ttagcgttac gaatgttggc gtaatcaaca
    23161 tgcaggccat cagcaataat accgcaataa atgtcagctt cgtcgaggat cgcgcccgcc
    23221 aggcccggtt cacgaccggt aatatacggc atcgcgttgt acagatgggt ggcaaaggta
    23281 atccccgcgc ggaaaccggc ttttgcttct ttcaacgtcg cgttggagtg accggcagaa
    23341 accacaatcc cggcatttgc cagtttgctg atgacttccg caggaaccat ttccggtgcc
    23401 agggtcactt tggtaatgac gtcggcgttt tcacacagga aatcgaccag cgcggcatca
    23461 ggcttacgca caaaattcgg attatgggtg ccttttttta ccagattcag ccacggacct
    23521 tccagatgca gacctaacgc ctgattcgga tgttttgcca ggtactcgcg cataacgcgc
    23581 acgccctgtt tcatcagctc atcgctggtg gtgataagcg tcggcagata gttagtacag
    23641 cctgatttct cattggcttt ctgcatgatt tccagcgttt ccacgctgac cgcttcagcg
    23701 gtgtcgttaa actgtacgcc gccgcagccg tttaactgca catcgataaa accgggggag
    23761 agaatggccc cgttcagtga acgttgttcg atctctggcg gcagttccgc taccggacag
    23821 acgcttttaa tcaggccatc agcgataaca accgcgtggt catcaagaaa ttcgtggccg
    23881 gtaaagatcc ggccctgggt taatgcatac attctgaccc ccgattttaa aaaatattgc
    23941 cctgagcaag gagccagggc agggataaca attacagacc tttgatattt tctgcttcta
    24001 attcattgaa atatcttaaa gtcttaactt tcagctccat ggtggaaggt tcatcgcaca
    24061 ccatgatcgc ttttggatgc agttgcagac agctgatggt ccacatgtgg ttcacgcaac
    24121 cttcaactgc agcctgcagt gccagtgctt tctggctacc cagcaccaga atcatcactt
    24181 cttcggcatc cagcaatgta ccaacaccga cagtcagggc atattttggc acctgattga
    24241 catcgttatc aaagaaacga gagttcgcga cgcgagtgtc atgagtcagg gttttgatac
    24301 gagtacgaga agccagagaa gacgccggtt cgttaaatgc aatatgaccg tcgttaccta
    24361 caccgcccat aaacagatga atttttccgt aagaacggat tttttcttca tactggcggc
    24421 actcggcgtc gatatccggg gcattgccgt tgagaaggtt gatgttttct gctggaatat
    24481 caacgtgatc gaagaaatta cggtgcataa agctgtagta gctttccgga tgctctttcg
    24541 gcagaccgac atattcgtcc atgttgaagg tgacaacgtg cttaaagctg acctggcctg
    24601 ctttatgcat ttcgactaac gctttatagg tggtcatcgg cgtgccgcca gtcggcaggc
    24661 ccagtacaaa cggacgatcg gcagtcggtt tgaacgcatt gatacgattg acgatatggc
    24721 gagcagccca tttgccgacc tgttcagcgg tagtcagggg gatcagtctc attattcacc
    24781 tcaataagta aaatgtaagc cgttggcgga ttaggcatct ttaagcgtaa cctggatttg
    24841 cgcagacagg cgtcaatccg acctgatttt ttgaatgata aaataagttt tctgctttag
    24901 ccagtaaaag ggagtgatga taacgatatt tggtgacaaa actcacaaaa gacacgcgtt
    24961 taatttgcga tacgaattaa attttcacac actctgtagc agatgatcta acaatctgat
    25021 tacagaacat cggcagtaca atttgcagca aaataaaaat acggctttaa acgagccaaa
    25081 tagggttctc gtagggggaa taagatgaat attttaggtt ttttccagcg actcggtagg
    25141 gcgttacagc tccctatcgc ggtgctgccg gtggcggcac tgttgctgcg attcggtcag
    25201 ccagatttac ttaacgttgc gtttattgcc caggcgggcg gtgcgatttt tgataacctc
    25261 gcattaatct tcgccatcgg tgtggcatcc agctggtcga aagacagcgc aggtgcggcg
    25321 gcgctggcgg gtgcggtagg ttactttgtg ttaaccaaag cgatggtgac catcaaccca
    25381 gaaattaaca tgggtgtact ggcgggtatc attaccggtc tggttggtgg cgcagcctat
    25441 aaccgttggt ccgatattaa actgccggac ttcctgagtt tcttcggcgg caaacgcttt
    25501 gtgccgattg ccaccggctt cttctgcctg gtgctggcgg ccatttttgg ttacgtctgg
    25561 ccgccggtac agcacgctat ccatgcagga ggcgagtgga tcgtttctgc gggcgcgctg
    25621 ggttccggta tctttggttt catcaaccgt ctgctgatcc caaccggtct gcatcaggtg
    25681 ctgaacacca tcgcctggtt ccagattggt gaattcacca acgcggcggg tacggttttc
    25741 cacggtgata tcaaccgctt ctatgccggt gacggcaccg cggggatgtt catgtccggc
    25801 ttcttcccga ttatgatgtt cggtctgccg ggtgcggcgc tggcgatgta cttcgcagca
    25861 ccgaaagagc gtcgtccgat ggttggcggg atgctgcttt ctgttgctgt tactgcgttc
    25921 ctgaccggtg tgactgagcc gctggaattc ctgttcatgt tccttgcacc gctgctgtac
    25981 ctcctgcacg cactgctgac cggtatcagc ctgtttgtgg caacactgct gggtatccat
    26041 gctggcttct ctttctcagc gggggctatc gactacgcgt tgatgtataa cctgccggcc
    26101 gccagccaga acgtctggat gctgctggtt atgggtgttg tcttcttcgc tatctacttc
    26161 gtggtgttca gtttggttat ccgcatgttc aacctgaaaa cgccgggtcg tgaagataaa
    26221 gaagacgaga tcgttactga agaagccaac agcaacactg aagaaggcct caatcaactg
    26281 gcgaccaact atattgctgc ggttggcggc actgacaacc tgaaagcaat tgacgcctgt
    26341 atcacccgtc tgcgcctgac cgtggctgac tctgcccgcg tcaacgatac gatgtgtaaa
    26401 cgtctgggtg cttctggtgt agtgaaactg aacaaacaga ctattcaggt gattgttggc
    26461 gcgaaagcag aatccatcgg cgatgcgatg aagaaagtcg ttgcccgtgg tccggtagcc
    26521 gctgcgtcag ctgaagcaac tccggcaact gccgcgcctg tagcaaaacc gcaggctgta
    26581 ccaaacgcgg tatctatcgc ggagctggta tcgccgatta ccggtgatgt cgtggcactg
    26641 gatcaggttc ctgacgaagc attcgccagc aaagcggtgg gtgacggtgt ggcggtgaaa
    26701 ccgacagata aaatcgtcgt atcaccagcc gcagggacaa tcgtgaaaat cttcaacacc
    26761 aaccacgcgt tctgcctgga aaccgaaaaa ggcgcggaga tcgtcgtcca tatgggtatc
    26821 gacaccgtag cgctggaagg taaaggcttt aaacgtctgg tggaagaggg tgcgcaggta
    26881 agcgcagggc aaccgattct ggaaatggat ctggattacc tgaacgctaa cgcccgctcg
    26941 atgattagcc cggtggtttg cagcaatatc gacgatttca gtggcttgat cattaaagct
    27001 cagggccatg ttgtggcggg tcaaacaccg ctgtatgaaa tcaaaaagta atctgcttta
    27061 tgcctgatgc gacgcttgag cgtcgcatcc aacaatgaca agcggtggag atcttctctg
    27121 ccgctttttt ttcatcaatc atccccataa tccttgttag attatcaatt ttaaaaaact
    27181 aacagttgtc agcctgtccc gcttataaga tcatacgccg ttatacgttg tttacgcttt
    27241 gaggaatcca cgatgagtga ggcagaagcc cgcccgacta actttatccg tcagatcatc
    27301 gatgaagatc tggccagtgg taagcacacc acagtacaca cccgtttccc gccggagccg
    27361 aatggctatc tgcatattgg ccatgcgaaa tctatctgcc tgaacttcgg gatcgcccag
    27421 gactataaag gccagtgcaa cctgcgtttc gacgacacta acccggtaaa agaagatatc
    27481 gagtatgttg agtcgatcaa aaacgacgta gagtggttag gttttcactg gtctggtaac
    27541 gtccgttact cctccgatta ttttgatcag ctccacgcct atgcgatcga actgatcaat
    27601 aaaggcctgg cgtacgttga tgaactgacg ccggaacaga tccgcgaata ccgcggcacc
    27661 ctgacgcaac cgggtaaaaa cagcccgtac cgcgaccgca gcgttgaaga gaacctggcg
    27721 ctgttcgaaa aaatgcgtgc cggtggtttt gaagaaggta aagcctgcct gcgtgcgaaa
    27781 atcgacatgg cttcaccgtt tatcgtgatg cgcgatccgg tgctgtaccg tattaaattt
    27841 gctgaacacc accagactgg caacaagtgg tgcatctacc cgatgtacga cttcacccac
    27901 tgcatcagcg atgccctgga aggtattacg cactctctgt gtacgcttga gttccaggac
    27961 aaccgtcgcc tgtacgactg ggtgctggac aacatcacca ttcctgttca cccgcgccag
    28021 tacgaattct cgcgcctgaa tctggaatac actgtgatgt ccaagcgtaa gctgaacctg
    28081 ctggtgaccg acaagcacgt tgaaggctgg gatgacccgc gtatgccgac catttccggt
    28141 ctgcgtcgtc gtggttacac tgctgcttct attcgtgagt tctgcaaacg catcggcgtg
    28201 accaagcagg acaacaccat tgagatggca tcgctggaat cctgcatccg tgaagatctc
    28261 aacgaaaatg cgccgcgcgc aatggcggtt atcgatccgg tgaaactggt tatcgaaaac
    28321 tatcagggcg aaggcgaaat ggtcaccatg ccgaaccatc cgaacaaacc ggaaatgggg
    28381 agtcgtcagg tgccgtttag cggtgagatt tggatcgatc gcgccgattt ccgcgaagaa
    28441 gctaacaagc agtacaaacg tctggtgctg ggtaaagaag tgcgtctgcg taatgcttac
    28501 gtcattaagg ctgaacgcgt ggagaaagat gccgaaggca atatcaccac catcttctgt
    28561 acttatgacg ccgacacctt aagcaaagat ccggcagatg gtcgtaaagt caaaggtgtt
    28621 attcactggg tgagcgcggc acatgcgctg ccggttgaaa tccgtctgta cgaccgtctg
    28681 ttcagcgtgc cgaaccctgg tgctgcggat gatttcctgt cggtgattaa cccggaatcg
    28741 ctggtgatca aacagggctt tgctgaaccg tcgctgaaag atgcggttgc gggtaaagca
    28801 ttccagtttg agcgtgaagg ttacttctgc ctcgacagcc gccattccac agcggaaaaa
    28861 ccggtattta accgcaccgt tgggctgcgt gatacctggg cgaaagtagg cgagtaattt
    28921 taagttctac caggccgggt gaggcattta cgtcgcatcc ggcaagaaat agcaaacaat
    28981 cccaaacgcc gcactctgcg gcgttttttc tgcttttctt cgcgaattaa ttccgct
//
LOCUS       DAZFJF010000056        29028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFJF010000056 DAZFJF010000000
VERSION     DAZFJF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.056"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            177..3062
                     /locus_tag="ACHKAU_002619"
     CDS             177..3062
                     /locus_tag="ACHKAU_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5531650.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(3162..5081)
                     /gene="dhaR"
                     /locus_tag="ACHKAU_002620"
     CDS             complement(3162..5081)
                     /gene="dhaR"
                     /locus_tag="ACHKAU_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO5531651.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5309..6379
                     /gene="dhaK"
                     /locus_tag="ACHKAU_002621"
     CDS             5309..6379
                     /gene="dhaK"
                     /locus_tag="ACHKAU_002621"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5531652.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6390..7022
                     /gene="dhaL"
                     /locus_tag="ACHKAU_002622"
     CDS             6390..7022
                     /gene="dhaL"
                     /locus_tag="ACHKAU_002622"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5531653.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7033..8451
                     /gene="dhaM"
                     /locus_tag="ACHKAU_002623"
     CDS             7033..8451
                     /gene="dhaM"
                     /locus_tag="ACHKAU_002623"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO5531654.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            8771..10468
                     /gene="treA"
                     /locus_tag="ACHKAU_002624"
     CDS             8771..10468
                     /gene="treA"
                     /locus_tag="ACHKAU_002624"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5531655.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(10547..10987)
                     /gene="ycgY"
                     /locus_tag="ACHKAU_002625"
     CDS             complement(10547..10987)
                     /gene="ycgY"
                     /locus_tag="ACHKAU_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO5531656.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(11165..11419)
                     /locus_tag="ACHKAU_002626"
     CDS             complement(11165..11419)
                     /locus_tag="ACHKAU_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5531657.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            11620..12354
                     /locus_tag="ACHKAU_002627"
     CDS             11620..12354
                     /locus_tag="ACHKAU_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO5531658.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(12356..12967)
                     /gene="emtA"
                     /locus_tag="ACHKAU_002628"
     CDS             complement(12356..12967)
                     /gene="emtA"
                     /locus_tag="ACHKAU_002628"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO5531659.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            13067..13981
                     /gene="ldcA"
                     /locus_tag="ACHKAU_002629"
     CDS             13067..13981
                     /gene="ldcA"
                     /locus_tag="ACHKAU_002629"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO5531660.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            14076..15812
                     /locus_tag="ACHKAU_002630"
     CDS             14076..15812
                     /locus_tag="ACHKAU_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO5531661.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16198..17268)
                     /gene="dadX"
                     /locus_tag="ACHKAU_002631"
     CDS             complement(16198..17268)
                     /gene="dadX"
                     /locus_tag="ACHKAU_002631"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO5531662.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(17278..18576)
                     /gene="dadA"
                     /locus_tag="ACHKAU_002632"
     CDS             complement(17278..18576)
                     /gene="dadA"
                     /locus_tag="ACHKAU_002632"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO5531663.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            18906..20438
                     /locus_tag="ACHKAU_002633"
     CDS             18906..20438
                     /locus_tag="ACHKAU_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO5531664.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(20490..21209)
                     /gene="fadR"
                     /locus_tag="ACHKAU_002634"
     CDS             complement(20490..21209)
                     /gene="fadR"
                     /locus_tag="ACHKAU_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO5531665.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            21431..22972
                     /gene="nhaB"
                     /locus_tag="ACHKAU_002635"
     CDS             21431..22972
                     /gene="nhaB"
                     /locus_tag="ACHKAU_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO5531666.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            23118..23648
                     /gene="dsbB"
                     /locus_tag="ACHKAU_002636"
     CDS             23118..23648
                     /gene="dsbB"
                     /locus_tag="ACHKAU_002636"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO5531667.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(23694..24962)
                     /gene="umuC"
                     /locus_tag="ACHKAU_002637"
     CDS             complement(23694..24962)
                     /gene="umuC"
                     /locus_tag="ACHKAU_002637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO5531668.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLSDTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(24962..25381)
                     /locus_tag="ACHKAU_002638"
     CDS             complement(24962..25381)
                     /locus_tag="ACHKAU_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5531669.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            25754..26665
                     /gene="hlyE"
                     /locus_tag="ACHKAU_002639"
     CDS             25754..26665
                     /gene="hlyE"
                     /locus_tag="ACHKAU_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO5531670.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(26872..27318)
                     /locus_tag="ACHKAU_002640"
     CDS             complement(26872..27318)
                     /locus_tag="ACHKAU_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO5531671.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(27410..28069)
                     /locus_tag="ACHKAU_002641"
     CDS             complement(27410..28069)
                     /locus_tag="ACHKAU_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5531672.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(28141..28434)
                     /locus_tag="ACHKAU_002642"
     CDS             complement(28141..28434)
                     /locus_tag="ACHKAU_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO5531673.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(28446..28604)
                     /locus_tag="ACHKAU_002643"
     CDS             complement(28446..28604)
                     /locus_tag="ACHKAU_002643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531674.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            28675..>29028
                     /gene="pliG"
                     /locus_tag="ACHKAU_002644"
     CDS             28675..>29028
                     /gene="pliG"
                     /locus_tag="ACHKAU_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO5531675.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDA"
BASE COUNT         7319 a         7404 c         7088 g         7217 t
ORIGIN      
        1 aaatatatat taattcctgt gttcctgatt ttgcccggta atagagaatt attctctgag
       61 aagctcatca tgattgcacc atacaaatat catgatgata agaggatatt attcattgat
      121 ttaaacaaaa tgacaaaatc catagaccca tagtctgggt actatatagg agtggtatgg
      181 gcatcaaaca acacaatggg aataccaaag cagatcgtct cgctgaatta aaaatccgtt
      241 cgccctcaat tcaactgata aaatttggcg ctattggttt gaatgcaatt atcttttccc
      301 ccctgctgat agctgctgat acaggaagtc aatatggcac caatattact attaatgaag
      361 gtgacagaat tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc
      421 cagcaggaaa cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg
      481 acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta
      541 accgactcac agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg
      601 actataccca tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca
      661 ttattgggca tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta
      721 taggcctaac catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca
      781 cgaccgatgg aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg
      841 ctgcgcgttt tacggctaca gacctgacaa tcgatgttca gggctacagc gccatgggga
      901 taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa cagttccatt aaaaccaatg
      961 gcgataatgc acacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg
     1021 atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg
     1081 cagatagcca tatttcttcc gcgcagggcg gtggcctcgt caccagtggt tcagacgcga
     1141 caatcaattt ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg
     1201 gtgcctcggc ccagacggca acggctgttg tcaacatgca aaataccgat attacagttg
     1261 atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag
     1321 acagtttggc tatcaccggc gcggcaggag ccaggggaat ttatgccatg accaacagcc
     1381 agatcgacct cacgagcgat ctggtcattg atatgagtac acccgaccag atggccattg
     1441 caacgcaaca tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta
     1501 tcaacggtag cgttctttcc aaaggtgggc taatcaatct agatatgcac ccagggtcgg
     1561 tttggacagg ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga
     1621 ataacagcgt ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt
     1681 caactgtcga ttttgccagc cacgggtcaa ctgccggcac atttgccaca ttaaacgtag
     1741 agaacctgag cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg
     1801 gcgttaataa taaaggggat ttattgaata tcagcggaag tagtgctggt aatcacgtat
     1861 tggctatccg caaccagggc agcgaggcca ctacgggaaa tgaagttctg acagtggtaa
     1921 aaaccactga cggtgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc
     1981 tgtacgatgt gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg
     2041 aaccgactcc taatcctgaa ccaacaccgg ctcccgctca gcctcccata gtcaaccccg
     2101 atcctacgcc tgaacccgat cctacgccta accccactcc cacacctaaa cccaccacga
     2161 ccgcagatgc tggcggcaat tatctcaatg tcggttactt attaaactat gttgaaaacc
     2221 gtacgctgat gcaacgaatg ggtgacctgc gaaatcagag taaagacggt aatatctggc
     2281 tgcgcagtta tgggggaagc ctggactcct tcgccagtgg caaactgagc ggctttgaca
     2341 tgggttacag cggtatccag tttggtgggg ataaacgtct ctctgatgta atgccgttgt
     2401 atgtcggtct gtatattggc tcaacacatg catcgccgga ctatagcgga ggcgacggta
     2461 ccgcacgttc agactacatg ggaatgtatg ccagttacat ggcacacaac ggtttttaca
     2521 gcgatctcgt tgtaaaagca tcgcgccaga aaaatagttt tcacgtactg gacagtcaga
     2581 acaacggcgt taacgccaac ggcactgcga atggattgag catctccctg gaagccgggc
     2641 agaggttcaa cctgacccct actggttatg ggttctatat agagccgcaa acccagctca
     2701 catacagcca ccagaatgag atggctatga aggcgagtaa tggtctcaat atacatctga
     2761 atcactacga atcgctgctg gggcgtgcca gcatgatact gggatatgac atcaccgcag
     2821 gcaacagcca gctgaatatg tatgtgaaga ctggagctat ccgcgagttt tcaggggata
     2881 cagactatct gttgaacaac tcccgggaga agtacagttt caaaggtaat ggctggaata
     2941 acggcgtggg agtcagtgca cagtataaca aacagcacac attctacctc gaagcggatt
     3001 acacgcagag taacctcttt gatcagaagc aagtcaacgg aggatatcgc ttcagctttt
     3061 aagtgcggga tagaccagcc cttaaacgat gctgaaaaat gttagcgtgc gccggaatga
     3121 aatatagatc actctgatgt atcagaaatc gtctctgtct ttcatacccg gcgcttaaac
     3181 tgccctgcat caatgccatg ttgcttcatt ttccgccaca gcgtagtgcg gccgatccca
     3241 agtaaagccg acatttcctg aatgcgaccg cctgtgacct gggctgcgtt aataattgcc
     3301 tctttttcaa cttccgcaaa tgacagactg gtggaaaggc gtgtggcgct gacatcatct
     3361 gttgcctgct cggtaaacag atgttccggc aaatcactga cgcgaatgcg cccattatca
     3421 ctactcagag caagattctc gatgacgctg taaagttcaa aatcgttgcc aggccatgca
     3481 caggaaacca ggcgagcgag ggcatcgtca tcaattttca gccgcgtaga gaagcgtttt
     3541 tcaagactgc gtaatttgtt attcaccagc gccggaatgc tgccacgccg catacgcaga
     3601 ggcgggatgg taatttcaaa tgcatgcaac gcgtaataca gctggcgact aaaacgattt
     3661 tgttccacca gcattgcgag gtccgcggtc gttgtggcaa tcactttgac atcaactggt
     3721 attaaacgcc gcgcatccag tcgcgtgata acaccctgct tgataacctg aagcaaagca
     3781 gactgtaact ccaccgccag atattcaatc ttttcaagaa acagcgtgcc gccgtgtgcc
     3841 agttccagcc gactcagacg gccattttca ttgtccgtgc gatcgccacc aataaattct
     3901 tccgccagcg ccgcatcacc atataactca caattgacgg cgatataagg acctgcagca
     3961 cgctcgcttt cattatgaat tgcctgactt agcagtgcct ttcccacgcc ctcttctcca
     4021 caaagcagga caggaaagct actgcgcgcc gcctggcgac caaaatgaat caagcggcgg
     4081 gtttgcggat cgtcctgtgg catatgagcg aaggtatggc tgacttttcc taattgactg
     4141 gtcatcaact gccgcatctg ttccacagga tggagcaaca aaataaagct ggttccctgc
     4201 gtttctatta tcggtttaag ggttatcacc gcatcaataa actgatgctg actttcaaag
     4261 gttgcttcta cgtgtttgag cggatgtgcc tgttttattg cttgttgcaa tacggcgggt
     4321 aacgttaaga gttcagtgat tgcccttccc tgacttgccg tcgcgtcaag gcgcaagacc
     4381 cgcgccgcct gggcattaat aaattgcaaa ttaccctgct cgtcccagct aatcacgcca
     4441 tcatccatac tttctaacag ggcattaagt tgatttaaat gacggttagt ttcagcgagc
     4501 aaactgtccg tcagcagtaa atttccgacc tcgcgggcga ttgccaacgt caacggcaaa
     4561 tcagctgcgg tagtttgctc aaccggacac gccagcgcta ttgttcccgt caatcggccc
     4621 ttgctgtcaa acaacggcgt tgcacaaaag gcccagttcc agagtgcctg tttgaaatgt
     4681 tgatcggcca tcgttttcac ggcctgacca gagatagccg ctaacgatag cgcacaagta
     4741 ccaataattc cctcggcgca atacgtgccg tcattgaatc ccagtgcact tagctgctgc
     4801 aaggtttgcg gatcgccatt acggctgaga atgcaggcgg tttcatcgag gataaacagc
     4861 gcacactctc gcggtgccat atattcccag gcatcttcca gcgcagcctg cccgagcgtc
     4921 agcatcgctt tcttacgccg ataaatagaa gcaaatgtca cgccctgggc ctgatgtggt
     4981 acgttccatg tctcccgttt catcagctta ttgcatcgct cccaggaggt tgcaattaag
     5041 ggagatatgc cccgaccatc gttgttaaaa gcgccactca tatcccgcat cccttatgac
     5101 aaaaggtgtt ccggctcgca attttaagta ttaatacacg taaaagtttc aatatggaac
     5161 attcattaaa gcatatgttt cactatgaaa cataatttgg gctaattttc ttttcccttg
     5221 cctgatgcac aatggattca tcggcagtca gtggtcgccg tgtcgttgaa catcatccat
     5281 gccctaccgt aattgctgga gcaaaataat gaaaaaattg atcaatgatg tgcaagacgt
     5341 actggacgaa caactggcag gactggcgaa agcgcatcca tcgctgacac tgcatcagga
     5401 tccggtgtat gtcacccgag ctgatgcccc tgttgcagga aaagtcgccc tgctgtcggg
     5461 tggcggcagc ggacacgagc cgatgcactg tggttatatc ggtcagggga tgctttcggg
     5521 ggcctgtccg ggcgaaattt tcacctcacc gacgcccgat aaaatctttg aatgctccat
     5581 gcaaattgat ggcggcgaag gtgtactgtt gattatcaaa aattacaccg gcgatattct
     5641 taactttgaa acagcgaccg agttactgca cgatagcggc gtaaaagtga ccactgtggt
     5701 cattgatgac gacgttgcgg taaaagacag tctttatacc gccgggcgac gcggcgttgc
     5761 caacaccgta ttaattgaaa aactcgtagg cgcagcggcg gagcgtggcg actcactgga
     5821 cgcctgtgcg gaactggggc gtaagttgaa taatcaaggc cactcaatag gtatcgctct
     5881 cggtgcctgt accgttcctg ccgcgggcaa accttctttt accctggcgg ataatgagat
     5941 ggagtttggc gtcggcattc atggtgagcc gggtattgac cgccgctcct tctcttccct
     6001 tgatcaaacc gtcgatgaaa tgttcgacac cctgctggaa aatggctcat accatcgcac
     6061 tttgcgtttc tgggattatc aacaaggcag ttggcaggaa gaacaacaaa ccaaacaacc
     6121 gctccagtct ggcgatcggg tgattgcgct ggttaacaat cttggcgcaa ctccgctttc
     6181 tgagctgtac ggcgtctata accgcctgac cacacgttgc cagcaagcgg gattgactat
     6241 cgaacgtaat ttaattggcg cgtactgcac ctcactggat atgaccggtt tctcaatcac
     6301 cttactgaaa gttgatgacg aaacgctggc actctgggac gccccggtcc acaccccggc
     6361 ccttaactgg ggtaaataag gagaaagcaa tgtcactgag cagaactcaa attgttaact
     6421 ggctcactcg ttgtggcgat attttcagca ccgagagcga gtatcttacc ggactggatc
     6481 gcgaaattgg cgacgctgac cacgggctaa atatgaaccg aggctttagc aaagtggtgg
     6541 aaaaactccc tgctatcgca gataaagata tcggtttcat tctcaagaat accggtatga
     6601 cgctgctttc cagcgtcggt ggtgccagtg gtccgctgtt cggtaccttc tttatccgcg
     6661 ccgcacaggc gacccaggca cggcaaagcc tgacactgga agagctttat cagatgttcc
     6721 gcgatggcgc ggacggcgta atcagtcgcg ggaaagccga acctggcgat aaaaccatgt
     6781 gtgatgtgtg ggtgccggtg gtggaatcgt tacgtcagtc cagcgagcaa aatctctctg
     6841 ttccggtggc gctcgaagct gccagtagaa tcgccgaatc cgctgcacaa agtacgatta
     6901 cgatgcaagc ccgcaaaggc cgcgccagtt atctcggtga acgcagtatt ggtcaccagg
     6961 atcccggcgc gacctcggtg atgtttatga tgcaaatgtt ggcgttagcc gcaaaagagt
     7021 aaggaattgg tgatggtaaa cctggtcata gtttcacata gcagccgact gggagaaggt
     7081 gtcggtgaat tagcccgtca gatgttaatg agtgatagtt gtaaaatcgc cattgccgcg
     7141 ggaattgacg atccacaaaa tcctattggt accgatgccg tcaaagtgat ggaggccatc
     7201 gaatctgttg ctgatgccga ccatgtgctg gtcatgatgg atatgggtag cgcattattg
     7261 agtgctgaaa ctgcgctgga attgctggct cccaagatcg ccgcaaaagt acgtttgtgt
     7321 gctgcgccgt tggtcgaagg tacactggca gcaacggtca gcgcggcctc gggagcggat
     7381 atcgacaaag ttatctttga cgctatgcat gcgctggaag ccaaacgtga acaactgggt
     7441 ttaccgtcct ccgacactga aatctctgac acatgtcctg cgtacgatga agaagcccgt
     7501 tctctggcgg tggtcataaa aaaccgtaac ggcctgcatg tacgtccggc ctcccggctg
     7561 gtttatacct tatcgacatt taatgccgat atgttgctgg aaaaaaacgg caaatgcgtc
     7621 acaccagaga gtattaacca gattgcgtta ctacaagttc gctataacga tacgctgcgc
     7681 ctgattgcga aagggccaga agctgaagag gcactgatcg ctttccgtca gctggctgaa
     7741 gataactttg gtgaaacgga ggaagtcgct ccacctactc tgcgtcccgt tccgcctgtt
     7801 tcgggtaaag ccttttatta tcaaccagtt ttatgtacgg tacaggcaaa atcaaccctg
     7861 accgtggaag aagaacaaga tcgattacgc caggctatcg acttcacgtt attagatctg
     7921 atgacgttaa cagcgaaagc agaagccagc gggcttgacg atattgccgc aatcttttct
     7981 ggtcaccata cactgttaga tgatccggaa ctgctggcgg cggcaagcga actccttcag
     8041 catgaacatt gcacggcaga atatgcctgg cagcaagttc ttaaagaact tagccagcaa
     8101 taccagcaac tggatgatga atatctacaa gctcgctata ttgatgtgga cgatcttctg
     8161 catcgcaccc tggtccacct gacccaaacg aaagaagaac tcccgcagtt taactcgcca
     8221 actattctac tggcggagaa catttatcct tccacagtac tgcaactgga tccggcggtt
     8281 gtaaaaggta tctgccttag cgccggaagt ccggtatccc acagcgccct aatcgcccgt
     8341 gaactgggga ttggctggat ttgccagcag ggtgagaaac tgtatgcgat acaaccagaa
     8401 gaaacgctaa cgctggacgt taaaacgcaa cgtttcaacc gtcagggtta agccaccgtt
     8461 cggatggcat cgttctgatg tcatccggca atttacccag cactgatgtt ttttaactac
     8521 ctgttatatt ttttaccaat ttatgatgat ttttcttcgc taatcgcgaa cccaacgtcg
     8581 ctgactggct aataaacaga cgttatttga tcactctatt tcaccgttat ttatcccttt
     8641 aattcccttt tctaaaatgc ctgacagttc gcagaatgag atttcgatca tgcagctagt
     8701 gcgatcctga actaaggttt tctgatactt gaataccgtt ttattcggtt tcgccaaagg
     8761 agaatgattg atgaaatccc ccgcaccttc tcgcccgcaa aaaatggcgt taattccagc
     8821 ctgtatcttt ttgtgtttcg ctgcgctatc ggtgcaggca gaagaaacac cggtaacacc
     8881 acagccgcct gatattttat tagggccgct gtttaatgat gtgcaaaacg ccaaactttt
     8941 tccggaccaa aaaacctttg ccgatgccgt gccgaacagc gatccgctga tgatccttgc
     9001 tgattatcgg atgcagcaaa accagagcgg atttgatctg cgccatttcg ttaacgtcaa
     9061 tttcaccctg ccgaaagagg gcgagaaata tgttccgcca gaggggcagt cactgcgcga
     9121 acatattgac ggactttggc cggtattaac gcgttctacc gaaaacaccg aaaaatggga
     9181 ttctctgtta ccgctgccgg aaccttatgt cgtgccgggc ggacgttttc gcgaggtata
     9241 ttactgggac agttacttca ccatgttagg acttgccgaa agcggtcact gggataaagt
     9301 cgcggatatg gtggccaatt ttgctcatga aatagacact tacggtcata ttcccaacgg
     9361 caaccgcagt tactatttaa gccgctcgca accgcccttc tttgccctga tggtagagtt
     9421 actggcgcag catgaaggcg atgccgcgtt gaagcaatac ctgccgcaaa tgcaaaaaga
     9481 atatgcttac tggatggacg gtgttgaaaa cctgcaagcc ggacaacagg aaaaacgcgt
     9541 tgtaaaactt caggatggta cccttctcaa ccgctactgg gacgatcgcg atacgccacg
     9601 accagagtca tgggtggaag atattgccac cgccaaaagc aatccgaatc gacctgccac
     9661 tgaaatttac cgcgacctgc gctctgccgc tgcgtctggc tgggatttca gctcgcgctg
     9721 gatggacaac ccgcatcaac taaatacctt acgcaccacc agcatcgtac cggtcgatct
     9781 gaacagcctg atgtttaaaa tggaaaaaat cctcgcccgc gccagcaaag ctgccggaga
     9841 taacgcgatg gcaaaccagt acgaaacgct ggcaaatgcc cgtcaaaaag ggatcgaaaa
     9901 atacatgtgg aacgatcaac aaggctggta tgccgattac gacctgaaaa gtcataaagt
     9961 gcgcaatcag ttaaccgcgg ccgccctgtt cccgctgtac gtcaatgcgg cagcgaaaga
    10021 tcgcgccaac aaaatggcga cggcgacgaa aacacatctg ctgcaacccg gcggcctgaa
    10081 caccacgtcg gtgaaaagtg ggcaacaatg ggatgcgcca aatggctggg caccgttaca
    10141 gtgggtcgcg acagaaggat tacaaaacta cgggcaaaaa gaggtggcga tggacattag
    10201 ctggcacttc ctgaccaatg ttcagcacac ctatgaccgg gagaaaaagc tggtggaaaa
    10261 atatgatgtc agcaccaccg gaacgggggg cggcggtggc gaatatccat tacaggatgg
    10321 ctttggctgg accaatggcg tgacgctgaa aatgctggat ttgatctgcc cgaaagagca
    10381 accgtgtgac aatgttccgg cgacgcgtcc gaccgttaag tcagcaacga cgcaaccctc
    10441 aaccaaagag gcacaaccca caccttaacc agcgcttact ccgacaacgt cattcggctt
    10501 ctccggatgg cgttgtcggc agataacacc atagctatgg tgttttctac acagacggtt
    10561 tttcaagtcc tggcgggaag tattgcgcaa atgtttgcca tacgtggttg ccgaagatag
    10621 cgtcatttct acgggtaagt gtttcacttt catcacccgg tgatttacac cgacgttgcc
    10681 tatataaagc atcgattgct acctgaatat tgttacggtg ttcactcatc aacgtttttt
    10741 tgctttctgg agctaaatta ataatcacat aatccagaca ctccagagaa tttagcaacc
    10801 aaaaaaagaa ttcagaccat tcctcttcgc gtaactgaag cattgccgaa cgggtatttt
    10861 ggcttaatct gccagaagga ttgatcaatt ctaaatgttc aataagcgca gccaaaaccg
    10921 cctcactatc cgccccattt ttaacgcctg actggctact gtctaaaata tttattttct
    10981 gaatcacacc aaaaacaacg aaacaataat taaaaaagga ttctgttgtt gttaccggga
    11041 accgacttta tcgggcatct aagtgctata ttgatttgtt ccacccgtaa agcaggtgtc
    11101 gagtatacgt tctgatgtga aagcggatag cggcatctgt atcgctatcc gccttatgaa
    11161 aatcttattc tcttcgcagg aggcggaata cgcccagaac gagaatagct cccaccaccg
    11221 ccaccaggaa gctgtgcaga ttaaaaccac tgatggagcc gccaatgcca aacatggtcg
    11281 ccagccagcc gccgaccacc gcaccgacta tcccgagaat acaggtcagg aaaaatccac
    11341 caccatcacg ccccggcatg attagcttgg cgataatgcc ggctatcagg ccaaaaataa
    11401 tccaggcaat aattcccatg ttcaggccct cgtttcgatg aatcggacgc aagaaatgct
    11461 tacgtcaacg ctctaagtat aggcaagggc gggatcgatg ctgggcgtat gtcggctgat
    11521 ttaagaacaa catgtaaaaa atattcacgc atttgtatta agttttgtta actgtgaccg
    11581 ataaaccaaa gacagtttgt cagtcaggag tttttccgcg tgagtcatta ccatgagcag
    11641 ttcctgaaac aaaatccgtt agccgtcctg ggcgtgttac gcgatttgca caaagccgca
    11701 attcctttgc gtctcagttg gaatggcggg cagctgatca gcaaaatact ggcaataacc
    11761 ccggataaac tggtgctgga tttcggcagt caagccgaag acaacatcgc cgtgctaaag
    11821 gcacagcaca ttaccattac cgccgaaact cagggtgcga aagtcgagtt tactgttgaa
    11881 caactacagc agagtgaata cttgcagctt ccggcattta tcaccgtacc gcctcccacc
    11941 ttatggtttg tacaacgacg ccgatatttc cgcatctccg ccccactcca tccgccttat
    12001 ttttgccaga ccaaactggc ggataacagt acgttacgtt tccgcctgta tgatttgtcg
    12061 ttaggcggca tgggcgcatt actggaaaca gcaaagcctg ccgaattaca agaaggcatg
    12121 cgcttcgctc agattgaagt caacatgggg caatggggtg tttttcactt tgacgcccag
    12181 ttaatctcca tcagcgagcg caaagtgatt gatggcaaga atgaaaccat caccactccc
    12241 cgtctgagct tccgttttct taacgtcagc ccgacggtgg agcggcaatt acagcggatt
    12301 attttctctc tcgagcgaga agcccgggaa aaagcggaca aagtgcgcga ctgatttaca
    12361 tcgcgtccag tgcctgctca agtttgtaga tatagcgcgg agcctgcggc gcaggatgat
    12421 ttcgcgctac gtgatcgagg aactcgtcag catccaaatc gttgattttg ctgatcgcct
    12481 ttttccgatc tgacgagaaa gtccgtagca gcgcacctgc cccgttagcg tatgacacca
    12541 ccagcgcata ttgcagtacc ttcggatctt caatgcctgc cagcgggccg gtttccagaa
    12601 tattcaggta agccgccccc attgaaatat tacgctctgg atttttcagc tcgctggtcg
    12661 tcggctcacc actccagccc atacggcgat aaacatcacg tccggaggtt gaagctttta
    12721 actgcatcaa accaatggca ttcgatttac tcaccgcatt cggattacca cccgattcga
    12781 tagcgataat cgccgtgatc aattgtggat cgacgcccca ggctgcaccg gctttctggc
    12841 ttattggcat ccactgcatc gcacgttgca ccggaacttt cgcgttccac ggcgggttcg
    12901 tatagtcatg ctttgatgaa caacccgcta ataacacaat caaaaaggca aaccatctca
    12961 atttcacttc ttctatcctt atagcctgga atcgccgttg aggtgacaac ggcacaccta
    13021 agcggcatga tatacatttc gtttctgttg tcagcaagga attgccatgt ctctgtttca
    13081 cttaattgcc ccatcgggtt actgcattaa acagcacgcc gcgctgcgtg gtatccagcg
    13141 ccttaccgac gaggggcatc aggtcaataa cgtcgaggtt attgcccgtc gttgtgagcg
    13201 ttttgcgggt acggaaacgg agcgcctgga agatctcaat tcccttgcca gactgaccac
    13261 ccccaacacc atcgtgctgg ctgtacgcgg cggttacggt gccagtcgtt tactggcaga
    13321 tattgactgg caggcactgg ttgctcgcca acaatatgac ccgttgctca tatgcgggca
    13381 tagcgatttt accgccattc agtgcggtct tctggcgcaa ggcaatgtca tcacctttag
    13441 cggccccatg ctggtagcaa actttggcgc ggatgaactg aacgccttta ccgagcacca
    13501 tttctggctg gcgttacgca atgaaacctt caccattgaa tggcaagggg aaggcccgac
    13561 atgtcagacc gaaggcacgt tgtggggagg caatcttgcg atgctgattt cactgattgg
    13621 tacaccgtgg atgccaaaaa ttgagaacgg tattctggtg ctggaagata ttaacgagca
    13681 ccctttccgc gtcgaacgta tgctgttgca gctctatcat gcgggaattt tgccacgtca
    13741 gaaggcgatt attctcggta gctttagcgg cagcacgccc aatgattatg acgcaggtta
    13801 caacctggag tcagtgtacg cgtttttgcg ttcccgcctg tcgattccac tcattaccgg
    13861 cctcgatttt ggtcatgaac aacgcacggt tacgctgccg ttgggcgcac atgccattct
    13921 gaataacacc cgggaaggca ctcagttgac gattagcggt catcctgttc ttaaaatgta
    13981 aaaaaatcac gcttcagggc taagtaaaac gctgtctctg ccgctaatat ggtagggttt
    14041 atataagaga cagcgtaatc aggagtaacc gaccgttgga tgccacaaca ataattagcc
    14101 ttttcatctt aggatccatc cttgtcacca gcagtatatt acttagttca ttttcttccc
    14161 gtcttggcat tcctattctg gttatttttc tggcgatcgg catgctggca ggagtcgatg
    14221 gcgtcggcgg tatcccgttt gataattacc ccttcgccta catggtaagt aacctggcac
    14281 tggcgattat tttgctcgac ggcgggatgc gcactcaggc cagttccttt cgtgtggcgt
    14341 taggaccggc actgtcgctg gcgacgctgg gcgtgcttat cacctctggt ttaaccggca
    14401 tgatggcggc gtggctgttt aatcttgatt tgattgaagg cttattaatc ggcgctatcg
    14461 tcggctctac cgatgctgca gcggtctttt ctctgctggg tggtaagggg cttaacgaac
    14521 gtgttggctc gacgctggaa attgaatccg gcagtaatga tccaatggcg gtctttctga
    14581 cgattaccct gattgcgatg atccagcaac atgaaagcag tgtcagttgg atgttcgtgg
    14641 tcgatattct gcagcaattt ggcctcggga ttgtcatcgg acttggtggt ggttatttac
    14701 tgctgcaaat gattaatcgc atcgccctgc ccgccggatt atatccattg ctggcattaa
    14761 gcggcggtat tttaattttt gctttaacca cggcgctgga aggcagcggt attctggctg
    14821 tttatctgtg cggttttctg ctgggtaatc ggccgattcg caaccgctac ggcatcctgc
    14881 aaaatttcga cggcctcgcc tggctggcgc aaatcgccat gttcctggtg ctggggctgt
    14941 tggttaaccc aagcgatctg ctgcctattg ccattccggc gctcattttg tcggcatgga
    15001 tgatattctt cgcccgtccc ctttcggtat ttgctggatt gctccccttc cgcggtttca
    15061 atctgcgtga gcgcgtgttt atcagctggg taggattacg cggcgcggtg ccaatcatcc
    15121 tggctgtgtt cccgatgatg gcggggctgg agaatgcacg tctgttcttt aatgtcgcct
    15181 tctttgtggt tctggtttca ctgctcttgc agggaacatc actctcgtgg gcggctaaaa
    15241 aagccaaagt ggtggttccg ccagtgggac gtccggtgtc acgcgttggc ctggatattc
    15301 atccggaaaa tccgtgggag cagtttgttt atcaattgag tgccgataaa tggtgcgtgg
    15361 gcgcggcact gcgcgatttg catatgccaa aagagacgcg tattgcggca ctgtttcgtg
    15421 ataaccagtt gcttcatcct accggcagca cccgactgcg cgaaggcgat gtgttgtgtg
    15481 taattggtcg ggaacgcgat cttccggcgc tcggtaaact gttcagccag tcgccgccgg
    15541 tcgcgctgga tcaacgcttc tttggtgact tcattctcga agccagcgcc aaatatgctg
    15601 atgttgcgct gatatatggc ctggaagacg ggcgagaata tcgtgataag cagcaaacgc
    15661 tgggtgaaat tgtccagcag ttgttaggcg cggcaccggt tgtcggtgac caggtagagt
    15721 ttgccgggat gatctggacg gtggccgaga aagaagataa tgaagtgttg aagattggcg
    15781 tacgggtggc tgaagaggaa gctgaatctt aaatttaatg aagcatctat cgccggttgc
    15841 gatgctttgc tgaacgcagc ctgagcctgc aattgtttgt cgtccataat ttaaccttca
    15901 tttgatgttg aattgaacat atcaaaatca ggcaattaca caaatctatg tacaggctcc
    15961 atcaagggta aagcgtgatt tatctgaagt cgagttcgag tcaacaatga agcgtaagcg
    16021 ttttatgtag aacgcctgtg cggtgagttc aggttccggg gaaatagtgt ccattctttt
    16081 aatggacact atcatgggca atggctctgt cacattaaca actacagttg ctgaccagcc
    16141 ggatggatga ttttttcgca accagaagac gctgcctccg atccggctta caacaagtta
    16201 cactgtcaca accgggacgc gtaacgccag cgcgcacatc aactcatagc ccaccgttcc
    16261 ggcagcggcg gcgacatcat caattttgat ctccttgccc cacagctcaa ccggcgtacc
    16321 aatccccgcc tgcgggcaag gcgttaaatc gaccgctagc atatccatcg agacggtccc
    16381 caccgtcatg gtgcgcacgc cgtccactaa aacaggggta ccggtaggcg cgtggcgcgg
    16441 ataaccgtcg gcgtaccctg cggcgacaat gccgattcgc tgttcatcgc gcgcagtata
    16501 gcgaccgccg tagcccacac gctcacccgc ttttagcgtc tggacaccaa taatctcact
    16561 gcttagcgtc atcaccggac gtaatccggt attggcgata tcacgccact gaccggacgg
    16621 cgaagcgcca tacaaaataa tgccaggccg aacccagtca aaatgcgctt ccgggtgcca
    16681 cagagtcgcc gccgaattgg acaacgaacg ccgacactcc agcccttccg ccgcctgctc
    16741 aatacgcgcc atcgcgctgg aaattccatc aggatgttcc gcttcggcaa aatgcgacat
    16801 cagggtcatc tcgccaacat tcgccattgc ccgcaactgc tgccagacgg taagcacgcg
    16861 atcgggctgg aagcccaacc gattcatccc actgttcact ttaagataaa tatccaacgg
    16921 tgcttttagc cgcgcatttt gcagtgcttt gagctgccag ttgctgtgta cgcaggtggt
    16981 caggcggtgc tggtcataaa tctccagatc ctgagcatgg aaaaatcctt ccagcatcag
    17041 gatcggcccc ttccagccgc gctcacgtaa cgttattgcc tcttccaggt taagtaatgc
    17101 aaagccatcg gtggccccga gcgcgctcca gatacgctca ataccatgcc cgtaagcgtt
    17161 cgcttttacc accgaccaga cgcgcgcgtg cggcgcggcc tggcggacaa tggacagatt
    17221 ctgttttaat gcctgcagat cgaggctggc ctgtatcgga cgggtcatct cgtttcctta
    17281 gctgtgtgcg ccatgtaaat ggcccggacg tgatggcgta aatccacggc tgtagcgcgc
    17341 tacgcttaga tcctcatatg ggatcgctgg cgtgcgacca gagagcagat cgcttaacaa
    17401 ctgaccggaa ccgcaagcca tcgtccagcc gagcgtgccg tgaccggtat tcagccacag
    17461 atttttaaag cgtgtacgcc cgacaaccgg cgtaccgtct ggcgtcatcg ggcgcaaacc
    17521 cgtccagaaa gtcgcctgct cgacatgacc gccgcgtgga tagagatcgc gaaccaccat
    17581 ttccagcgtt tcacgacgcg gttgcaacag ctcggtgtta aaaccaacaa tttccgccat
    17641 tccgccaaca cgaatgcggt tatcgaaacg ggtaatggcg attttgtagg tttcatcaag
    17701 aatagtggat actggcgcac catcttcctg cgcaattgga atagtcagcg agtagccttt
    17761 cagcggatac accggaatat caacaatgcc tttgagcatc gccgtcgagt aagaaccaaa
    17821 cgccatcaca tacgcatcgg ccttaatcac ttcatcgcca cacttcacgc cgtagatttg
    17881 ctcgccgtcg caaagcagtt ggtcaacggg cgtattaaag cggaatttaa cccccgcctg
    17941 ctctgccatc cgcgccagat tctgggtaaa cagctggcag tctccggttt catcattggg
    18001 cagttgcaga ccacccgtca gtttgtgcgc tacttctgcc agcgcgggct ccacttccgc
    18061 caggcggctg gattccagca gctgatacgg tacgccggca tcttccagca cggcgatatc
    18121 gcgggtcgca ttttcatact gttgttcggt acggaacagt tgcagcgtcc caccctgacg
    18181 cccttcatac tgaatattgg tttcggcgcg caatgctttc aggcaatcac ggctgtattc
    18241 cgccagacgc accatccgcc ctttgttttc catgtagtgg ctggtgtcgc agttacgtaa
    18301 catttgccac atccatttca actgaaactg cgtaccgtcg agacggaccg ccagcggcgc
    18361 atggcgctgg aacatccatt tgatcgcttt taaaggcaca cctggtgccg cccacggtgc
    18421 agcatatccg ggggagattt gcccggcatt cgcggcgctg gtttccaggg cggcccccgg
    18481 ctcccgatca atgacggtga cctcatgtcc tgcctgattt aagtaccagg cgctggcaac
    18541 gcctaccaca ccacttccca gtatgacaac tcgcatagcc actccgataa cggtagatac
    18601 agtaaaagaa taataatcta attacatctt gataactcag ttgaaaatat tattcaacat
    18661 acggcttttt tatggtgagc tggctcacat ctccctgact ctgcggcaat atggcgtctt
    18721 tagtatctcc tgctgaacgc cctttttatc cagcataaaa ttccgttcag aagcggatta
    18781 gtggcactct gataataaga aatcgcaaag aaaaaaattc tccggcgacg cggtttttaa
    18841 caggtgttct atgcttgaaa tgaggtgctc cacacagaga gcgccgacaa caatgaggtg
    18901 cgcgtatggc gacgatcgat tctatgaata aggacaccac acgtttgagc gatggacccg
    18961 actggacgtt cgacctgctg gatgtttatc tggcagagat agaccgggtg gcgaaactct
    19021 accggctgga tacctacccg caccagattg aagtgataac ctcagaacag atgatggatg
    19081 cctactccag cgtcggcatg ccaattaact atccgcactg gtcattcggt aaaaagttta
    19141 tcgaaactga acggctgtat aagcacggtc agcaaggact ggcctatgaa atcgtcatta
    19201 actctaaccc gtgtatcgct tacctgatgg aagagaacac cattaccatg caggcgctgg
    19261 tgatggcaca cgcctgctat gggcataact ctttctttaa aaacaattac ttattccgca
    19321 gttggaccga cgccagttcg attgtcgatt atctgatttt tgcccgtaaa tatattaccg
    19381 agtgcgaaga acgttatggc gttgatgaag tagaacggct tctggactcg tgccacgcgc
    19441 tgatgaacta cggcgtggac cggtacaaac gcccgcaaaa aatctcactg caagaagaga
    19501 aagcccggca gaaaagtcgc gaagagtatc tacaaagtca ggtcaatatg ctctggcgta
    19561 ccctgccgaa gcgcgaggaa gagaaaacgg ttgctgaagc gcgccgctat ccgtccgaac
    19621 cacaagaaaa cctgctctat tttatggaga aaaatgcgcc actgctggaa tcatggcagc
    19681 gtgaaatcct gcgtattgtg cgtaaggtga gccagtattt ttatccgcaa aaacagactc
    19741 aggtgatgaa cgaaggctgg gcgaccttct ggcactatac catccttaac catctgtatg
    19801 atgaagggaa agtaacggaa cgttttatgc tggagttttt gcacagccac accaatgtgg
    19861 tcttccagcc cccctataac agcccgtggt acagcggcat caacccgtat gccctcgggt
    19921 tcgccatgtt ccaggatatt aaacggattt gtcagtcgcc aacggaagaa gacaaatact
    19981 ggttcccgga tatcgccggt tccgactggc tggaaacgct gcatttcgcg atgcgtgatt
    20041 tcaaagatga gagttttatc agccagtttc tgtcaccgaa agtgatgcgt gatttccgct
    20101 tcttcaccgt gctggatgat gatcggcata attatctgga gatttccgct attcataatg
    20161 aagaaggtta tcgggagatc cgtaaccggt tatcgtcgca atataactta agtaatctgg
    20221 agccgaatat tcagatctgg aacgtggatt tgcgcggcga ccgttcgctg acgctgcgtt
    20281 acattccaca taatcgcgca ccgctggatc gggggcgcaa agaagtgctg aagcatgtgc
    20341 atcgcctgtg gggatttgat gtgatgctcg aacagcaaaa cgaagacggc agcatcgagt
    20401 tgctggaacg ttgcccgcca agaatgggaa atctgtaaaa acaacaaaaa acccctcgtt
    20461 tgaggggttt gctctttaaa cggaagggat tatcgcccct gaatggctaa atcacccggc
    20521 agatttttct gcatccggtg ccaaatctcg ccactctcat gcccatagcg acgcactgtt
    20581 tcgtacacct gatcgtgcgc gccttcactg cacaacgccg acagtttgtg gtagaagccc
    20641 agcgccagac tgcgcgcttc cggattggcg aaatagtgac gaccaatgcg cgtatacagc
    20701 cctttcatcc cgttaaggat cagaccgtaa atcgggttgc cggaagcaaa cgccaggccg
    20761 cggaatatgt tgtaatccag ctcggcaaag gcatcggcgt gatcggccac ttcattagcg
    20821 gtagccagca cttcctgcgc tttatcggga tgctgacgaa acgcggtgcg aataaaaata
    20881 gtggaaatat tggtacgcac cgacagcaaa ttatcaataa gctgcggcac actttcgtga
    20941 tccagtcgcg ccagtgtttc aaggatattt aaaccagagg tttcccagaa attattcacc
    21001 ttcgtcggct tgccatgttg aatggtcaac cagccatctc gtgccagacg ctgtaacact
    21061 tcacgtaacg tagtacgcgt tacgccaatt aattctgaaa gttcacgttc tgcgggcaaa
    21121 atagtcccgg gagggaagcg gttattccag atactttcaa taatgtactc ttccgcgaaa
    21181 cccgccgggc tttgcgcctt aatgaccata gtgagatttc cataacacag caaaacaaag
    21241 ttggactcat cataccagag ggctaactac gctgatagca gacaaaaaga agaaaaaagg
    21301 gatcaaggct tcattttccg catctctttc cccttgacta tcaagacttg cctcactcct
    21361 gcctgaccgt taagctttgc gtttaaacat aaaaacatca ttgattattt gtaaggtaag
    21421 ggaaaccatc atggagatct cctggggccg cgcgctatgg cgcaattttt tgggccagtc
    21481 ccccgactgg tacaaactcg ccctcatcat tttcttaatc gtaaacccgt taattttcct
    21541 catcagccct ttcgtcgcgg gctggttgct ggtagcggaa tttattttca ctctggcgat
    21601 ggccctgaaa tgctacccgc tgctccccgg tggtctgttg gctatcgaag cggtattcat
    21661 cggcatgacc agcgcggaac acgtccgtga agaggtggcg gcaaatcttg aagtcttgct
    21721 gttattgatg tttatggtgg cgggtatcta ttttatgaaa cagctgttgc tgttcatatt
    21781 tacccgtttg ctgttaagca ttcgctccaa aatgctgctg tcgctctctt tttgcgtggc
    21841 ggcggcgttc ctctccgcgt tcctcgatgc cttaaccgtc gtggcggtgg tgatcagcgt
    21901 tgcagtcggt ttttatggta tttatcaccg cgtagcctct tcccgtactg aagataccga
    21961 cctgcaagac gatagtcata tcgacaagca ttacaaagtg gttctggaac agttccgtgg
    22021 ctttctgcgt agcctgatga tgcatgccgg tgtcggcact gcattaggcg gcgtgatgac
    22081 catggtgggc gaaccacaga acctgatcat cgctaaagcg gctggctggc attttggcga
    22141 tttcttcctg cgcatgtcgc cggtgaccgt tccggttctg atttgtggcc tgttaacctg
    22201 cctgctggta gagaagctgc gttggtttgg ctacggtgaa acgctgccgg agaaagtccg
    22261 cgaagtgttg caacagtttg acgatcaaag ccgccaccag cgtactcgtc aggataaaat
    22321 ccgtctgatt gtccaggcga ttattggcgt ctggctggtg actgcgctgg cgttgcatct
    22381 ggcggaagtt ggcttgattg gtttgtcagt cattattctg gcgacatcat tgaccggtgt
    22441 caccgatgaa catgctatcg gtaaagcctt caccgaatct ctgccattca ccgcactgtt
    22501 gacggtgttt ttctcggtag tcgcggtgat tatcgaccaa caactgtttt cgccaattat
    22561 tcagtttgtg ttgcaggcat cggaacatgc tcagctgtcg ctgttctata ttttcaacgg
    22621 tctgctgtca tccatttcgg ataacgtctt cgtggggacg atttatatca acgaagcgaa
    22681 agcggcaatg gaaagtggcg ctatcacgtt gaagcaatac gagctgctgg cggtcgccat
    22741 taataccggt accaatctgc cctccgtcgc tacgccgaac ggtcaggctg cgttcctgtt
    22801 cctgctgacc tctgcactcg cgccattgat tcgcctctct tatggccgca tggtgtggat
    22861 ggccctgcct tacaccctcg tactgacact cgtcggcttg ctctgcgtcg agtttacgct
    22921 tgcccctgta accgaatggt ttatgcaaat gggctggata gcaacgcttt gataacaact
    22981 taccgggcat ataaatgccc ggtttgcctt ttcgcccgat aattgtccaa ttgcgtccta
    23041 tttttgctgc ctcctggtgg cgcagcgaat gaattggttt aaactgcgca ctctatgcat
    23101 attgcaggga aatgattatg ttgcgatttt tgaaccaatg ttcacaaggc cggggcgcgt
    23161 ggttgttgat ggcgtttact gctctggcac tggaactgac ggcgctgtgg ttccagcatg
    23221 tgatgttact gaaaccttgc gtgctctgta tttatgaacg ctgcgcgtta ttcggcgttc
    23281 tgggtgctgc gctgattggc gcgatcgccc cgaaaactcc gctgcgttat gtagcgatgg
    23341 ttatctggtt gtatagtgcg ttccgcggtg tgcagttaac ttacgagcac accatgcttc
    23401 agctctatcc ttcgccgttt gccacctgtg attttatggt tcgtttcccg gaatggctgc
    23461 cgctggataa atgggtgccg caagtgtttg tcgcctctgg cgattgcgcc gagcgtcagt
    23521 gggatttttt aggtatggaa atgccgcagt ggctgctcgg tatttttatc gcttacctga
    23581 ttgtcgcggt gctggtggtg atttcccagc cgtttaaagc gaaaaaacgt gacctgttcg
    23641 gtcgctaatc cattcggcgc tcctgcggga gcgctttttt cctgccgcta tatttatttg
    23701 acccgcagta aatcagaact tcgcgttgta taacgtggtg aaagcatggc acgcttcatc
    23761 tgccattgtt gctggatccc ctgcccggca aaatagagtg ttcctctgcc ctctttagca
    23821 ttcagtgtat ccattaccgt catcaattgc tcactcccgg ggcgcggtgc gttgtcatcg
    23881 aataaattga gctgcgcgac tccctgactg aagaaatccc ccagcatcac gcccgctttt
    23941 tggtagcgat ggcccgcttg ccagatggca tccagagatc gcgtagcagc gttaatgata
    24001 tccctgctgt cctgagtggg cgtcagcagt tttaccgacg cgctattgcc gtaataaggt
    24061 tcattgagcg caaatggtga cgtcttaata aatgtggaga taaaccgaca atattgatgc
    24121 tcgctgcgaa gtttttccgc cgcccgggca gcgtaactac aaatggcctg ccgcatcgat
    24181 ggataatccg tgatgcgttc accaaacgag cgggaacaga taatttcctg cttcgtcggt
    24241 gcaaactctt ccagttgcaa acagggttcg ccgcgcagtt cacgcaccgt tctttcgagc
    24301 acgacattaa aatgtttacg gataaaccgg atatctgtat ccgacaaatc aagaacggtt
    24361 ttgatcccca tcgcgtccag ttttttgctg atccgccgtc caatccccca gacttcatcc
    24421 acggggagag cagacattaa tttacgctgg cgttccagat ttgataaatc caccaccccc
    24481 cccgtctgcc gctgccattt ttttgccgca tgattggcaa gcttagccag cgttttggtc
    24541 tgggcgatcc ccacaccaac agtaagatgg gtacgttgta gcaccgttgc gcgaatttct
    24601 ctgccaaaat cagtcagatc gcgacaatta cgcacacctg tcagatcgca gaatgcctca
    24661 tcaatactgt aaatctcgac gcggggcgat agttcttcca gcgtcgacat cacccgattg
    24721 ctcatgtctg cgtaaagctc ataattgctg ctaaagcaaa ccacaccaca gcgacgaaac
    24781 agatcttttt gtttgaacca gggatcgccc attttaacac ccagcgcctt tgcctcagcg
    24841 tttcgggcga taacgcaacc gtcattattc gatagcacaa ccaccggttt accccataaa
    24901 tcagggcgaa acaccgtctc acagctggca taaaacgcgt ttacatcaca gagagcaaac
    24961 atcagcgcat cgccttaacg acgtggatca ccacaccaaa gacatccagc gtatcttcgc
    25021 tactgatggt aatgggcgag tacgcactgt tcatgggaat aagctgtacc gtcgggcgta
    25081 gttgcaattt tttcaccgta aactcgccgt caacagcagc gatgacaata tcaccatggc
    25141 tggcggtaat agcgctatcg acaatcagta aatcaccgtc actaattcca ccatcaatca
    25201 tagaatcacc acttgctttg acgaagtaag tcgcgctggg atgctggatc aacagttgat
    25261 tcagatcgat gcgctgttca acgtaatctg ctgccggtga aggaaagcca cactgaacaa
    25321 gatcgctaaa tagcggaaaa gtcacaattt cgcggagatc cgcaggcttg ataaacaaca
    25381 taataatctg cctgaagcta tactgttttt atatacagta gcctgttctt gccagcagat
    25441 caatactgat tcaggctatc aatatttgtc gctgcataga ctgctgattt ttcgttctct
    25501 tatcctgtgc tcacgcggcc ttctggcgac tacgctcatc cagcagaaat gaaaaatatt
    25561 acccggctaa aaaatagaat agaagcattc gccataatga cattaaacat tgtttgatat
    25621 ttatcatatt aatagaaata aagacattga cgcatcccgc ccggctaact atgaattaga
    25681 tgaagtaaaa tttattaata gttgtaaaac aggagtttca ttacaattta tatatttaaa
    25741 gaggcgaatg attatgactg aaatcgttgc agataaaacg gtagaagtag ttaaaaacgc
    25801 aatcgaaacc gcagatggag cattagatct ttataataaa tatctcgatc aggtcatccc
    25861 ctggcagacc tttgatgaaa ccataaaaga gttaagtcgc tttaaacagg agtattcaca
    25921 ggcagcctcc gttttagtcg gcgatattaa aaccttactt atggatagcc aggataagta
    25981 ttttgaagca acccaaacag tgtatgaatg gtgtggtgtt gcgacgcaat tgctcgcagc
    26041 gtatattttg ctatttgatg agtacaatga gaagaaagca tccgcccaga aagacattct
    26101 cattaaggta ctggatgacg gcatcacgaa gctgaatgaa gcgcaaaaat ccctgctggt
    26161 aagctcacaa agtttcaaca acgcttccgg gaaactgctg gcgttagata gccagttaac
    26221 caatgatttt tcagaaaaaa gcagctattt ccagtcacag gtagataaaa tcaggaagga
    26281 agcgtatgcc ggtgccgcag ccggtgtcgt cgccggtcca tttggattaa tcatttccta
    26341 ttctattgct gcgggcgtag ttgaagggaa actgattcca gaattgaaga acaagttaaa
    26401 atctgtgcag aatttcttta ccaccctgtc taacacggtt aaacaagcga ataaagatat
    26461 cgatgccgcc aaattgaaat taaccaccga aatagccgcc atcggggaga taaaaacgga
    26521 aactgaaaca accagattct atgttgatta tgatgattta atgctttctt tgctaaaaga
    26581 agcggcaaaa aaaatgatta acacctgtaa tgagtatcag aaaagacacg gtaaaaagac
    26641 actctttgag gtacctgaag tctgataagc gattattctc tccatgtact caaggtataa
    26701 ggtttatcac atttaaaaac acagacgttt tttaaagatt gagagttacc tgttgataat
    26761 aaggtatcgg gtaactctca ttatgacaga gcgctttcaa aaacagggct acgaaatggc
    26821 gagcatccag acgtatatta agaaagcccc catcaacatc tattaaattt atcactgcgc
    26881 ccagtcaggt ttatttaaga tatgatcctg ccagtcaatc acttctgatt ctttcactgc
    26941 gatatgacgc acagagatac gttcaccatg cattgccgct ttcgaacccg taagtagcgg
    27001 atgccacgca ggtaaatctt taccttccgc cagcaaccga taagcgcagg tcattggcag
    27061 ccattcgaat gttggcagat tttcacgggt taatttaatg cagtcgggtt caaactcgaa
    27121 acgacgttcg tagttccgac attgacaggt tttaatattg agctggcgac aggcgacgtt
    27181 agtgaagtag atttcgtcgg tgtcttcatc catcagttta tgcaggcaac actgaccgca
    27241 accatcacac aacgactccc attccgcatc gctcatttcg tccagggttt tactttgcca
    27301 gaaaggtaca tcgctcatca ggtgttccgc cattacgtta aaacgcacct tataaccagt
    27361 ctggcacagc gatgcaagtt ttgccgccgc tttcaggcgg caaaaagtat tacaaaacgc
    27421 gagttgccaa agaatgccca tcgaaagtga ctgtcagctc atcaccgctt tgcaacgggc
    27481 cgacgccatc aggcgtgcct gtcagcacaa cgtcaccggc cttgagggta aaaaacttgc
    27541 tcatataagc gatcagcgga acaattttat ggatcatgtc cgcagtcgta ccttgctgac
    27601 gttgctcgcc gtttacgctc aggcccagcg ttgtattttg cgggtcgccg gtaaattccg
    27661 ccgcgggaat aaacccggaa agcggacaag agttatcaaa cgctttagcc ttttcccacg
    27721 gctgcccggc tttcttcatt tttccctgaa catcacgcaa tgtcagatcg agcgccacgc
    27781 cataaccggc aatggctttg cggacatgct cttccgtagc ctgacgcagc gtcgcgccaa
    27841 tcaacaccgc cagttcgact tcatgatgaa ctgaaccgaa atcggatggg atcgccaatg
    27901 gctgccgcag atcgcacagt gccgtttctg gtttaataaa cagcactggc tcttcgggca
    27961 ctgcgctgcc catctcttta atatgtttgg catagttact gccaacacag actactttac
    28021 tcaccggata atccagcagc gcaccttgcc agttgtgatg ttgatacata acgttcccct
    28081 caccttatcg tggatatgaa aacccgcgcc gacaaagata atgccaccgc cggatatcgg
    28141 ttatttgtta gtgtcgtctg ttttctgccc catgacggaa agatgttgct tcagcaaatc
    28201 ttcgggtggt ggcggtaact gcaaatagta accttgctcg gttaatgcca gtttaacctt
    28261 ttcaatatca gcattgacca gtttcttccg cccatccaac ggcagaatca tcgctaactg
    28321 aggctgacca aaacctttca tcagttcctc aggaacacgc gaaaaatcgt cttttttttc
    28381 gacatataaa taggtctggt cacgcttgct gcttcgataa atcacacaaa acatactttt
    28441 actctttaag atgccaggtt ttggcataag ctaacatcaa tgaaacccgc gccacaagcc
    28501 agccagctgt agatttctac ggtttgttga gtccatgccc ctttataagt aatccccccg
    28561 gcaaaaatgc cgcgccggga ttttaccgaa cgcaggaaag ccactggaat tctcccgtta
    28621 gggattgtta tattataaca gttcatcgta ctcattctga acaggagact accaatgaaa
    28681 atcaagagca tcagtaaggc tgtattgctg tttgcactgc taacctccac gagctttgct
    28741 gcgggtaaga atgtcaatgt tgagttcaga aaaggccata gcagcgctca gtactccggc
    28801 gaaataaagg gatacgatta cgatacatat accttctatg ccaaaaaagg tcagaaagtg
    28861 catgtgagta tctctaatga aggcgctgat acttacctct tcgggccagg aatcgatgat
    28921 tcagttgatt tatccagata ttccccagaa ctcgatagtc acggacaata ctcactccct
    28981 gcttctggca aatatgagct gcgagtgttg caaacccgaa acgacgcc
//
LOCUS       DAZFJF010000057        28881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFJF010000057 DAZFJF010000000
VERSION     DAZFJF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.057"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            85..939
                     /locus_tag="ACHKAU_002645"
     CDS             85..939
                     /locus_tag="ACHKAU_002645"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO5531676.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            975..1865
                     /locus_tag="ACHKAU_002646"
     CDS             975..1865
                     /locus_tag="ACHKAU_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5531677.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            1926..3425
                     /locus_tag="ACHKAU_002647"
     CDS             1926..3425
                     /locus_tag="ACHKAU_002647"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5531678.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            3426..4415
                     /locus_tag="ACHKAU_002648"
     CDS             3426..4415
                     /locus_tag="ACHKAU_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5531679.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            4437..5399
                     /locus_tag="ACHKAU_002649"
     CDS             4437..5399
                     /locus_tag="ACHKAU_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5531680.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            5396..6442
                     /locus_tag="ACHKAU_002650"
     CDS             5396..6442
                     /locus_tag="ACHKAU_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO5531681.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(6534..7883)
                     /gene="accC"
                     /locus_tag="ACHKAU_002651"
     CDS             complement(6534..7883)
                     /gene="accC"
                     /locus_tag="ACHKAU_002651"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO5531682.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7894..8364)
                     /gene="accB"
                     /locus_tag="ACHKAU_002652"
     CDS             complement(7894..8364)
                     /gene="accB"
                     /locus_tag="ACHKAU_002652"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO5531683.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9229..10059)
                     /locus_tag="ACHKAU_002653"
     CDS             complement(9229..10059)
                     /locus_tag="ACHKAU_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531684.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(10096..11070)
                     /locus_tag="ACHKAU_002654"
     CDS             complement(10096..11070)
                     /locus_tag="ACHKAU_002654"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO5531685.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            11222..13162
                     /gene="csrD"
                     /locus_tag="ACHKAU_002655"
     CDS             11222..13162
                     /gene="csrD"
                     /locus_tag="ACHKAU_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO5531686.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13467..14510
                     /gene="mreB"
                     /locus_tag="ACHKAU_002656"
     CDS             13467..14510
                     /gene="mreB"
                     /locus_tag="ACHKAU_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO5531687.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            14576..15679
                     /gene="mreC"
                     /locus_tag="ACHKAU_002657"
     CDS             14576..15679
                     /gene="mreC"
                     /locus_tag="ACHKAU_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO5531688.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            15679..16167
                     /gene="mreD"
                     /locus_tag="ACHKAU_002658"
     CDS             15679..16167
                     /gene="mreD"
                     /locus_tag="ACHKAU_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO5531689.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            16176..16769
                     /gene="yhdE"
                     /locus_tag="ACHKAU_002659"
     CDS             16176..16769
                     /gene="yhdE"
                     /locus_tag="ACHKAU_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO5531690.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            16759..18228
                     /gene="rng"
                     /locus_tag="ACHKAU_002660"
     CDS             16759..18228
                     /gene="rng"
                     /locus_tag="ACHKAU_002660"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO5531691.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            18296..22096
                     /gene="yhdP"
                     /locus_tag="ACHKAU_002661"
     CDS             18296..22096
                     /gene="yhdP"
                     /locus_tag="ACHKAU_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO5531692.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            22252..23697
                     /gene="tldD"
                     /locus_tag="ACHKAU_002662"
     CDS             22252..23697
                     /gene="tldD"
                     /locus_tag="ACHKAU_002662"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO5531693.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(23825..24754)
                     /gene="aaeR"
                     /locus_tag="ACHKAU_002663"
     CDS             complement(23825..24754)
                     /gene="aaeR"
                     /locus_tag="ACHKAU_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO5531694.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24937..25140
                     /gene="aaeX"
                     /locus_tag="ACHKAU_002664"
     CDS             24937..25140
                     /gene="aaeX"
                     /locus_tag="ACHKAU_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO5531695.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            25148..26080
                     /gene="aaeA"
                     /locus_tag="ACHKAU_002665"
     CDS             25148..26080
                     /gene="aaeA"
                     /locus_tag="ACHKAU_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO5531696.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            26086..28053
                     /gene="aaeB"
                     /locus_tag="ACHKAU_002666"
     CDS             26086..28053
                     /gene="aaeB"
                     /locus_tag="ACHKAU_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO5531697.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            28145..28417
                     /locus_tag="ACHKAU_002667"
     CDS             28145..28417
                     /locus_tag="ACHKAU_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO5531698.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(28473..28736)
                     /gene="yhcN"
                     /locus_tag="ACHKAU_002668"
     CDS             complement(28473..28736)
                     /gene="yhcN"
                     /locus_tag="ACHKAU_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO5531699.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6984 a         6880 c         7750 g         7267 t
ORIGIN      
        1 tgctgtgcta gtttgctttc aaaatgaaag tgaatattct ctttcttcat tttgtaaggc
       61 gaacgacaaa taacaggagc tgcgatgtac atcatttcaa gcaaaaacat gctgaaaaaa
      121 gcccagtcag agggatatgc cgtccccgct ttcaacattc ataaccttga aactttacag
      181 gtagttgttg agagcgctgc acaacttcgc tctcccgtta tgctggcggg aacacccggt
      241 acttatcgtt atggtggcgt gggaagttta atttcgttag tacaatcgct ggctcgcgag
      301 tacaacctgc cgttagtcct gcatctcgac catcatgaag agagtgatga catttttaac
      361 aaggttcgcg cagggatccg ttctgtcatg attgatggct cccatttgcc atttgaagac
      421 aacattgcgc gagtgcaaga agttaccacc ttttgccatc gctttgatgt tagcgttgag
      481 gccgagctag ggcgtcttgg cggccaggag gacgatctgc tggtggatga aaaagattcc
      541 gcttatacct caccagccgc agctgcagag tttgttcaac gtacccaaat tgactccctg
      601 gccgtagcaa tcggcacagc acatggttta tatgcgcatg aaccacgact ggattttgtg
      661 cggctggaac agattcgtca gcgagtggag attccactgg tgttgcatgg tgcttcgggc
      721 ttatcagaac aggacgttca gcactgtatt cgccgcggta tttgtaaagt gaatgtcgcg
      781 acagaattga aaattgcgtt tgccgatgcg ttaaaaggat atttctacaa caatcctgcg
      841 gcaaatgatc cacgccacta tatgcagccc gcaaaggccg ccatgaaaga agtcgtaact
      901 cgtattattg gtgtctgtgg tagtgaagga aaaatttaag acgttgtacc ctacaaaaac
      961 aatgaggatt gtttatgaaa aatattaaat tacttggttc agctatgttg gtgagcctgg
     1021 cattgttctc gcaaaattcg ttggcaaaag agtataaaat tggtgcgtca ttattaacac
     1081 agcaacatcc tttctatatt gatcttgcga atgccatgaa agaagaggca aaaaaagaca
     1141 atgtcaattt aagtgtctct attgccaatc aggatttaaa caaacaactt tctgatgttg
     1201 aggattttat tactaaaaaa gtggatgcca ttattatctc tccggtcgat tcgaaaggcg
     1261 tccaggcggc gataattaaa gccgataagg ccggtattcc cgttatcacc gtcgacgtcg
     1321 ctgcggaagg cgttccggtc gtatcgcatg ttgcgaccga taactatgcg ggaggcgttg
     1381 aagcaggcaa gttaatgggc aaacttttga atggtaaagg tactgtagcc attatttctt
     1441 atccggcact acaatcggtg gttgcgcgcg ttgatggttt taagaaaggg ttaagcgata
     1501 caccggatat taaaattgtt gctgaacagc ctggtattac gcgggctgaa gcgttaacga
     1561 ctgcgcaaaa cattatgcag gcaaatgcca acctgaatgg tctgtttggc tttggtgatg
     1621 atgcggcgct ggcagcggtt atcgcagcaa aatctgcgca taacgacaac atcaaaatca
     1681 ttggttttga cggcatgaaa gaagcgcgtg atgccgttga cagtgaaaaa acgtttgcgg
     1741 ccgtgattcg gcaatatccc gatcaaatgg gtgctaaagc cattgatgcc gctgttgatc
     1801 acctcaatgg taaaccggta gaaaaaatga tcccggtaca acccggcgtt tatacaggta
     1861 aatagtaaac atcttcacct cacaacaaaa cgttattggc gttttgttgt gacaccgagg
     1921 tcattatgac ggatgttatt cttgatgttt cgcatattgc gaagacattt ggtcatgttc
     1981 aagcattaaa agatattacc ctctctttac gtaaagggcg ggtgcatact ttattaggcg
     2041 agaatggtgc cggtaaatcg acattaatga aaatactggc cggtgtttat ccgccaaccc
     2101 agggaacgat aacactacgt ggtgaaacta tcaccattaa taatccgcag cattcacgcc
     2161 agttaggaat tgcgattatt tttcaggaat tgagcctgag taataatatg accgtggcgg
     2221 aaaatattta tgccaataat gaaccgcgac gttttggcat tattaacgat aaaaaaatgc
     2281 tcgcagattg ccagaatctc ctcgcggaac tgggcatacc actagaccca ctagaaatgg
     2341 tcggcaatat gtcgatggca cataggcaac tggtagaaat tgctaaagcc ttaagctatg
     2401 cagcggatgt cgtgattatg gatgaaccga catcttcgct aagtgataat gaagcggaga
     2461 ttctgtttaa cattattgaa aaattaaagc agcgaggctg tgcggttatt tatatctctc
     2521 atcgcatgga tgaaattatg cggatttcag atgatatttc ggtgatccgc gacggtgagt
     2581 atatcgccac gcatgaaaag aaaaacagtg atatccagca tttgattgcc caaatggttg
     2641 gtcgggaaat gaaaaatatt tggccagcac gtcttgggga aataccggat gaaaatgttc
     2701 ctgcaaaact ggaagtaaaa aatctttccc atccttcgct ttttaaagag atcagtttcg
     2761 ctgtgcgtcc gggggaggtc ctcggatttt ttggcctggt gggggccggg cgttccgatg
     2821 tgatgaaggc gctctttggc ctcgtttcgt atcaggggac tgtgttgatt gatggtaagg
     2881 aagtcaggat cgctaatcct aaacaggcta ttgatcatgg cattgctttt gtgaccgaaa
     2941 accgcaagga agaagggtta gtgctgatgc acgacgtcaa tatgaacacg caccatgtgg
     3001 cgtttcaata taacgcttcg cggatgggac tcatcaatca ccgacaagaa gaggcgaaga
     3061 cgctgcaatc aattgccaga atgaacacta aagtgagttc tgtacatcag gcagtgggag
     3121 cgctgagcgg cggtaatcaa cagaaaatcg tgttatcgaa gtggctggag aaaactccgc
     3181 gtattttatt gctcgatgag ccaacgcgcg gagtggatgt cggcgcaaaa tttgaaatct
     3241 ataacgtgat acgtcaactg gctgcggcag ggacagcaat tattctggtg tcctctgaac
     3301 tgccagaagt catggcactt agcgatcgtc tggtggtaat gcgtaataaa acgattgccg
     3361 atatttattc atgtgaaaat ttaacgcaga cacaggtaat gaccgctgca acaggagtac
     3421 gttaaatgac agagaaaact cttgccagtc cgaaaccttc ggaaattccc attgcaaata
     3481 attttaaaaa agtcttcagg caatatggcg gtatcctcag tggaatggtg gtgttaatca
     3541 ttctgttcag ttttattaat gacagttttt ttaccgccaa caacattact aatattattc
     3601 ttcaggtttc gataattgca attaccgcct atggaatgac ttatgtactt ttgcttggcg
     3661 atattgattt atcggtcggt tcaaccattg cattgattgg cacatttgct gcgcttgggg
     3721 cttcatgggg aattccattt attttattgg tcccattatc cattattgca gcactggctc
     3781 tggggatgat aaatggtggc ttgacggcta tagcggggat cccgtcgttt attgtcactg
     3841 ttgctactat ggggatcttc cgtggtatcg cttatatagt gaccgatggt atgcccatca
     3901 tgattaaaga tgatgccttt ctggcactgg ggaatggaga atttctctat atacctattc
     3961 caatctggat attaatcatc ttattgctga ttaaccactt cattcttact aaaacaacct
     4021 ttggacgaaa aatttatatc actggtggta ataaagaagc ggcaatttat tcagggataa
     4081 atgtcacccg cttgaaaatt aaagtcttta tgattacagc cgtgctggca ggtatcagcg
     4141 ggatgatcct cgcctcacgc cgctattccg ggcaaccgaa ttccgcgttg agctacgaac
     4201 tggatgctat tgctgcggct gtactgggag gcaccagcct gaacggcggc tatggaaccg
     4261 tggtggggac ggtgatcggc gcattaacta tcggcgttat taataatggc atgaacttaa
     4321 tgaatgttcc ctatttttac cagatggtgg ttaaaggatt agtcattctt gtagccgttt
     4381 attttgacgt aagaaataaa agaaaacgca gctaatcatt tcaaggaatt aatcatatga
     4441 gtaaggtctt cacaattgga gaaattctgg ttgaaattat ggcgagtaaa atcggccagc
     4501 catttgatca accaggtatc tggaacggcc cttatcccag tggtgcgccc gctattttta
     4561 tcgaccaggt gacacgtctt ggcgttccgt gcggcatcat tagctgtgtg gggaatgatg
     4621 gctttggtga tattaatata catcgtctgg ctgctgacgg tgtggatatc agggggattt
     4681 cagtcttacc tctggaagct accgggagtg cctttgttac ttatcataac tctggcgatc
     4741 gcgattttat ttttaatatt aagaatgccg cttgcggtaa attatcggcg cagcatgttg
     4801 atgaaaatat tctgaaggac tgtacccatt ttcatattat gggctcttcc ctgttttcgt
     4861 ttcatatggt agacgcagta aaaaaagcgg taactatcgt taaagcaaat ggtggtgtta
     4921 tttctttcga ccctaatatt cgtaaagaga tgctcgatat tccagaaatg cgtgacgccc
     4981 tccactttgt tcttgagtta acggatatct atatgcccag cgagggcgaa gtgttgctct
     5041 tgtcgccaca ttcgacgccg gagcgtgcaa tcgcaggttt tcttgaagaa ggtgttaaag
     5101 aggtgattgt gaagcgcggc aatcaaggtg ccagttacta ctccgcgaat gagcagtttc
     5161 atgttgagag ctatccggtg gaagaagttg acccgaccgg tgcgggagac tgctttggtg
     5221 gcgcgtggat tgcctgccgc cagctagggt ttgacgccca tcgagcgctg caatatgcca
     5281 atgcctgcgg tgcgctggcg gtaaccagac gtggcccaat ggagggaacg tctcgccttg
     5341 cggaaatcga agcgttcatc cagcgccatg acatgtccat tcgggaagca gcacaatgaa
     5401 aaacgaaatt tggtcgtgga ttggcgatct ctcgcaggtt gccggaatta aacactatga
     5461 gttacgtagt ggcagagcaa agggtacaga agcgtttgat gtccgtactg gtgcgggact
     5521 ggcatttacg gtggtaaaag atcgcgcgct ggatattgca tgggccagct acaaggacac
     5581 ggcgctttct tttatcaccc ccaacggcat tgtcgccccc gctttttttg agtctcaggg
     5641 caacggattt ttacgtagct tttacgcggg gttactgact acctgcggac ttagctacat
     5701 tggcacgcct tgtgaggatg aaggagaaac gctggggtta cacgggcgtt tggctgcgac
     5761 tccagcagaa gaggtggggt atcgcacaga acggaccgac gacggcattg aatttgtcat
     5821 caatggcaaa gtacgggaaa cgcgactgtt cggcgaaaat ctgacgctgg aaaggaccat
     5881 tcgctgccgt tatggcgaaa atgtgctgcg cattgaagac aaggtgacta atcatggttt
     5941 cacccgccag ccgctgcaaa ttctttatca ctttaattac ggctggcctt tattatcgcc
     6001 acaggcagaa atcttgctgt cggctaaaag cgtaacgcca cgaactccgc atgcggcgga
     6061 agggttaacc agtcatctgg agatttgtac cccacagccc ggttttgacg agcaggttta
     6121 ctatctcacg ttgaatagcg acagtcaggg gatgagtaaa gttgctctgg tgaacgccga
     6181 actggggtgg ggaatttacg agaaattcga caccatgcag ctacccaact ttatccagtg
     6241 gaaaaattta ggcgcgggtg agtatgttat gggactggag gtcagtaata gttttcctga
     6301 cggtcgtgat aaagaacgag cgcaggggcg tctgcccttt attgagccag gggaaacaaa
     6361 gaaatactgc tttgagctgg gaattgttga tggcgatgct gaaatgagcg cgttgaaagc
     6421 ggaaattgca ggctaccgtt agctaacggc aataaaaacg ccgggcagcg ttaacaggcc
     6481 cggcgttgat tacgttcttt gctaagcgcg atgaaaacac cgcgcgtcac aaattatttt
     6541 tcctgaagac cgagtttttt ctccagatag tggatgttag tgccaccatg ctggaagttc
     6601 tcgtcattca tgatgcggat ctgcagatca acgttggttt tgataccgtc gatgatcagc
     6661 tcctgcagcg cattcttcat gcgggcaatc gccacgtcac ggttttcacc gtagcaaatc
     6721 agcttaccga tcattgagtc atagtacggc ggtacggtgt agcccgcgta gatatgagac
     6781 tcccaacgta cgccaaaacc gccaggtgcg tggaaacggg tgattttgcc tgggcttggc
     6841 aggaaggtgt tcggatcttc ggcgttgata cgacattcca ccgcatggcc gcgaacgtga
     6901 acttcttctt gcttgatcga cagcggttga ccggcagcga tacgcagctg ttctttgatc
     6961 aggtcaacgc cggtgatcat ttctgtaacc gggtgttcta cctgaatacg ggtgttcatt
     7021 tcgatgaaat agaactcgcc gttttcgaac aggaactcga aagtacctgc accgcgatag
     7081 ccgatatcaa cacacgcttt agcgcaacgt tcgccgatgt agcgacgcag ttccggggta
     7141 atgcccggtg ctggtgcttc ttcgaccact ttctggtggc ggcgctgcat ggagcagtca
     7201 cgttccgcca gatagatagc gttgccctga ccgtcagcca gtacctgaat ctcgacgtgg
     7261 cgaggatttt ccaggtattt ctccatgtaa accatatcgt tgctgaaagc agctttcgct
     7321 tccgcacggg tcatggagat ggattgtgcc agttcagcgt cgccgcgcac tacgcgcata
     7381 ccgcgaccac cgccgccgcc ggaggctttg ataatcaccg gataaccaat gcgtttagca
     7441 atggcacggt ttttatccat atcgtcgccc aacgggccgt cagaacccgg tacgcaaggg
     7501 acgcccgctt ttttcatcgc cgcgattgcg gatactttgt cgcccatcag gcgaatggtt
     7561 tctgctttcg ggccgatgaa gataaagccg gagcgttcaa cctgctcggc aaagttggcg
     7621 ttctcggaga ggaagccgta acccggatgg attgctactg cgccggtgat ttcagcggcg
     7681 ctgatgattg ccgggatgtt cagataactt tttactgacg gagcagggcc aatacagacc
     7741 gtttcatctg ccagtaatac gtgttttaga tcgcgatccg cgctggagtg cacagcgaca
     7801 gtcttgatgc ccagttcttt acaggcacga agaatacgca atgcaatctc gccgcggttg
     7861 gcaataacaa ttttatccag catgttcgcc tcgttactcg atgacgacca gcggctcgtc
     7921 aaattctacc ggttgtccac tttcgaccag aattgctttc acggtaccgg atttgtccgc
     7981 ttcgatctgg ttcatcattt tcatggcttc aacgatgcac agggtatcgc ccacgttgac
     8041 tttctgaccc acttcgatga acgcttttgc gtccgggctt ggggtgcggt agaaagtacc
     8101 aaccatcggg gaacgtacga tgtgaccact gatttccgct gctgctggcg cttccatgga
     8161 aggaacggtc gccggagcgg ctgcgttaga ttgagctggc tgctgcatta ttggtgcagc
     8221 gtaagcttgt tgcatcacag ggaaacttgc ggcaggagct gcacggctaa tgcgtactga
     8281 ctcttcgcct tcagaaattt ccagttcgga gatgcctgat tcttcaacca gctcgatcag
     8341 ttttttaatc ttacgaatat ccatgagtgg gttccgtact ctttgtttag tgtgattgtg
     8401 acaggcgttt caccgccgtc tgtaaagcgt atgccccgac agctgtatgc atagcgataa
     8461 attccagcag gccgggacgc cggtctattt tgccccagag tgtcaatgtt agacttgacg
     8521 gacattgtgc agcgatctgc tgctatcctc cggcaaaaaa caaaatatac ttcgttgcgc
     8581 caatgtcacc ttttccgcag cgcaaaaact gcgtcaggga ggacgaggtc gcacattata
     8641 acgatttcgt agcaattggc agctaaatac tggtcttatc agggaagata atcaacagct
     8701 aacatgtaaa taaccttcaa caccgtgtaa tttgcaacaa gccgcacaat tcacgaaatt
     8761 agcgccatca tcgacggaac ttcttataac gtaaggtgaa aagctgtaaa gccagccctg
     8821 cgaagatgag gggctgcaat gagataatct tcacagacca caaataatgt agggatgcca
     8881 ggatcgcgac aagatagacg acattacgca gcggttgctg gggattaccc aattttcact
     8941 gcattgtctg gatagatgtt aatgctaaag cacgcaaaat taaccgattg acaatgctca
     9001 gcattaaata ggttcgagtg attaatttgt agtcaaacaa aatcagatta ttccccccat
     9061 tttgcagaag gtaaactgtt ttgtcaggcg gcagtgcggc tatctgatgc tttcagaatt
     9121 tgccacctga caataaaaaa gccgatttgg tagcaaaatc ggcttcggtg agtttgcagt
     9181 tttcatcaca tactccgaca agctttttca agcatgacgg aggttgcgtt aatgcaaggt
     9241 ttctacccag ggataaaagt gaccgttacg gcggatgaat atctgtgttg tcagaggatg
     9301 gtcatcctca cggaagcatt gttttgttag ctgtcgtaat gcactgagat ttgctggctg
     9361 atcattgtgg gtgaaaaaca gttttcctct ggagggaacg gtcactaaca ggtcgccgca
     9421 ttcaggatcg ggtgattgca cccaaagatc gtcaatgagc atcagactgg gctcataagt
     9481 accccctgcg gagataacgg caacttttcc atcgtttgcg cctgtatcaa cgatttcagc
     9541 cgtaacttcc ggcaatattt gcttcaaatt actgatggct tgttcgcggt caatggtcac
     9601 ttcgtagcgc atcagatagc gcatggtttc gctaccatct tcagcaaaca agataattaa
     9661 ctcatcgtta aagtcttccc aggcaaaatc cagcgaggcg ttgaggacct ctggatcaga
     9721 ctcatactgg ctaagctgtt ggcgaacggt atccagccag gctttttctt taatcaccgg
     9781 aacaatacag cgcccggtcg gcagcatttc tgcaagctgt ttcagcatat ctgcagccac
     9841 ttcttgcagc gggtctggtg cggcttgata acgtttgtag gcatcatcaa gggtgatttc
     9901 caccgtttgg tgacccgtat caatgcgcat caatttgtgg tcagtcgaaa tcatgacttt
     9961 ccagtttgga tgatgttgta caacttcctg accaaggtgt tcgacgaaag ctgtccggct
    10021 aaccgctttt cccgttaacc gattgaataa tgatgtcaaa atgagttccc tctcttatta
    10081 ttccttgcta aatggttagt taaccttcac cagcgtgcga ccctggatct ggttattaat
    10141 gatggcctcg gcaaagttcg gtgcctctga cagagatatc tcttttgccg cctgggtata
    10201 gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct tgtgcgcggc gttctggtgg
    10261 cgtcattact gaatccaccc cttgcaaacg gacattacgc agaataaatg gcatgaccgt
    10321 ggttggcaga gtaaaaccac ccgccagacc acaggcagcc acgcagccgc cgtaattcat
    10381 ttgcgccagc acttttgcca gcactttgtc gccaacagtg tcaattgccc cagcccagac
    10441 ttgtttttcc agaggacggg attcggcaaa ctcatcacga gggagaatac gactggcacc
    10501 taaacttttc agatattcat gggtactttc gcgaccggaa acggcaacga cctgataacc
    10561 caacttatgc agcagcgcca cggcggtact gccgacgcca ccactggcac ccgtcacgac
    10621 aatctccccg tcctgcgggc gaacaccggc atcttccagc gccatcacac acagcatggc
    10681 ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg tccagccctt gcggcatggc
    10741 aaccagccag tcacctttca ctcgcgcctg ctccgccagc cccccccagt ggttttcacc
    10801 aacgccccag ccagtgagta acacctcctg accggcatga aaacgcggat cttcgctggt
    10861 gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga aaattacgga tgatttttcc
    10921 cttaccggta atcgccagcg catctttata gttcaggctc gaccagtgaa catcaaccgt
    10981 gacatcgccc tccggcaggc gactttcgtc aagtgtctgt accgatgcga gagttttgcc
    11041 gtcctgctgt tctaaaagta acgcctgcat aagtggtcct catgtgcatg atggattgga
    11101 aaataatttc tgaagactat actcgctaat ggaaaagcaa agccgatgaa gcgcaataaa
    11161 ttgcgagata aatctgattt gctagtatgc ccgcttcctc actatcggag ttaacacaag
    11221 gatgagatta acgacgaaat tttcggcctt tgttacgctg ctcaccgggt taacaatttt
    11281 tgtgactttg ctgggctgtt cgctaagttt ctacaacgca attcagtata agtttagtca
    11341 tcgcgttcag gcggtggcga cggcgattga tacccacctt gtgtcgaatg acttcagcac
    11401 attaaggcca caaattaccg aattaatgat gtcggcagat atcgttcgtg tagacctgct
    11461 ccatggtgat aaacaggttt ataccctggc cagaaatggt agttatcgtc cggttggctc
    11521 cagcgatctg tttcgcgaac tgagtgttcc gttgataaag catccgggga tgtcgctgcg
    11581 tctggtttat caggatccga tgggcaacta tttccattct ttaatgacta ccgcgccgct
    11641 cacgggggcg attggcttta tcattgttat gctcttcctg gcggtacgct ggttacaacg
    11701 gcaacttgcc gggcaagaat tgctggaaac ccgggctact cgtatcttaa acggtgagcg
    11761 tggctctaat gtgttgggaa ccatctatga atggccgccc agaaccagca gtgcgctaga
    11821 tacgctgctt cgtgaaattc agaacgcacg cgaacaacac agccgtcttg atacgctgat
    11881 ccgctcttat gccgcccagg acgtgaaaac cggcctcaat aaccgactct ttttcgataa
    11941 tcagttagca acgttactgg aagatcagga gaaagtaggt acccacggga tcgtgatgat
    12001 gattcgtctg ccggatttca atatgttgag cgatacttgg gggcacagcc aggttgaaga
    12061 acagttcttc actctgacga atctgctgtc gacatttatg atgcgctacc ctggcgcact
    12121 gctggcgcgt taccaccgca gtgattttgc tgcgctgtta ccgcaccgga cgttaaaaga
    12181 ggcagagagc atcgccggtc agttaatcaa agccgtcgat accttgccga acaataaaat
    12241 gctcgatcgc gacgatatga tccacattgg tatctgcgcc tggcgtagtg gtcaggatac
    12301 cgagcaggta atggaacatg cagagtctgc cacgcgtaat gcgggattgc agggcggcaa
    12361 tagctgggct atttacgatg actcgttgcc tgaaaaagga cgcggtaatg ttcgctggcg
    12421 tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc ctttatcaaa aaccggcggt
    12481 tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc cgcatcttcg atggtaatga
    12541 agaggttagc tcggcggagt atatgccgat ggtcttgcag tttggcttat cggaagagta
    12601 tgaccgtctg caaatcagcc gtcttattcc actattgcgt tactggccag aggaaaatct
    12661 ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt tttcagcgtt ggctgcgcga
    12721 tacgttaatg caatgtgaaa aatcacaacg aaaacgcata attattgaac ttgcagaggc
    12781 cgatgtaggt caacatatca gtcgtttaca acctgttatt cgtttagtga atgctttagg
    12841 ggtacgggta gccgtcaacc aggctggttt gacgctggta agcaccagtt ggatcaaaga
    12901 acttaatgtt gagttactca agctccatcc ggggctggtc agaaacattg agaagcgaac
    12961 ggagaaccag ctgctggttc aaagcctggt ggaagcctgc tccgggacca gcacccaagt
    13021 ttacgccacc ggtgtgcgtt cgcgaagcga gtggcagacc ctgattcagc gcggtgttac
    13081 aggcgggcaa ggggattttt tcgcgtcctc acagccactt gatactaacg tgaaaaaata
    13141 ttcacaaaga tactcggttt aacctgccgt ttaatctgtt ttcacgtaga ataacgcgcg
    13201 ctgcgtctca tgggagtgtg cttgtctgct cgccagattg ttgcagcaca tatgcagatg
    13261 aatgacctta cgcggttgca aacaggcgag gaatgctgct gatgcattaa gcctttctgg
    13321 actcaggcag agatttgtaa caaaggaaac gaactgcact aattttcacc gtagcagatg
    13381 atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt aagcggattt tcttttccgc
    13441 cccagctttc aggattatcc cttagtatgt tgaaaaaatt tcgtggcatg ttttccaatg
    13501 acttgtccat tgacctgggt actgcgaata ccctcattta tgtaaaagga caaggcatcg
    13561 tattgaatga gccttccgtg gtggccattc gtcaggatcg tgccggttca ccgaaaagcg
    13621 tagctgcagt aggtcatgac gcgaagcaga tgctgggccg tacgccgggc aatattgctg
    13681 ccattcgccc aatgaaagac ggcgttatcg ccgacttctt cgtgactgaa aaaatgctcc
    13741 agcacttcat caaacaagtg cacagcaaca gctttatgcg tccaagcccg cgcgttctgg
    13801 tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc aattcgtgaa tccgcgcagg
    13861 gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat ggctgccgca attggtgctg
    13921 gcctgccggt ttctgaagcg accggttcta tggtggttga tatcggtggt ggtaccactg
    13981 aagttgctgt tatctccttg aacggcgtgg tttactcctc ttccgtgcgc attggtggtg
    14041 atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa ttacggttct ctgatcggtg
    14101 aagccaccgc agaacgtatc aagcacgaaa tcggttcggc ttacccgggc gatgaagtcc
    14161 gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt tccacgcggt tttaccctga
    14221 actccaacga aatcctcgaa gcactgcagg aaccgctgac cggtattgtg agcgcggtaa
    14281 tggttgcact ggaacagtgc ccgccggaac tggcttccga catctccgag cgcggcatgg
    14341 tgctcaccgg tggtggcgca ctgctgcgta accttgaccg tttgttaatg gaagaaaccg
    14401 gcattccagt cgttgttgct gaagacccgc tgacctgtgt ggcgcgcggt ggcggcaaag
    14461 cgctggaaat gatcgacatg cacggcggcg acctgttcag cgaagagtaa tcggatgcag
    14521 gcaggggaaa tgtctgttta ccctgcctgg tctgatacga gaatacgcat aacttatgaa
    14581 gccaattttt agccgtggcc cgtcgctaca gattcgcctt attctggcgg tgctggtggc
    14641 gctcggcatt attattgccg acagtcgctt ggggacgttc agtcaaatcc gtacttatat
    14701 ggataccgcc gtcagtcctt tctactttgt ttccaatgct cctcgtgaat tgctggatgg
    14761 cgtatcgcag acgctggcct cgcgtgacca actagaactt gaaaaccggg cgttacgtca
    14821 ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga caatacaaac aggagaacgc
    14881 gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat gagcagaaaa tggtgactca
    14941 ggttatctcc acggttaacg atccttatag cgatcaagtt gttatcgata aaggtagcgt
    15001 taatggcgtt tatgaaggcc agccggtcat cagcgacaaa ggtgttgttg gtcaggtggt
    15061 ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt gatgcgaccc acgcgctgcc
    15121 aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc ggtaacggtt gtacggatga
    15181 tttgcagctt gagcatctgc cagcgaatac ggatattcgt gttggtgatg tgctggtgac
    15241 ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc gcggttgtct cttccgtaaa
    15301 actcgatacc cagcgcgctt atactgtgat tcaggcgcgt ccgactgcag gactgcaacg
    15361 tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac ggcgctaacc cgatgacgcc
    15421 ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag atgatgccgc aggtattgcc
    15481 ttcgccagac gcgatggggc caaagttacc tgaaccggca acggggatcg ctcagccgac
    15541 tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct gctgcgccca cacagcctgc
    15601 tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt ggtgctcaac cgcctgcgcg
    15661 tgcgccggga gggcaatagt ggcgagctat cgtagccagg gacgttgggt aatctggctc
    15721 tctttcctca ttgcgctgtt gctgcaaatc atgccctggc cggataacct gattgttttc
    15781 cggccaaact gggtgttact catcttgttg tattggatcc tggccttgcc tcatcgcgta
    15841 aatgtgggca caggttttgt gatgggtgcc atactggatc tgatcagcgg ctcgacgctt
    15901 ggcgtacgcg tattggcgat gagcatcatt gcttacctgg tggcgctgaa ataccagctt
    15961 ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca tgttgctttc gctggtggtg
    16021 gatattattg ttttctgggc agagttttta gtgattaacg tctctttcag accggaagtg
    16081 ttctggagta gtgtagtcaa tggggtgctc tggccgtgga ttttcttgct gatgcgtaaa
    16141 gtccgtcagc agtttgcagt gcaataaagg tttctatgac ttctctgtat ttagcttccg
    16201 gttctccgcg tcgtcaggag ttacttgcgc aacttggcgt gacctttgaa cgtattgtta
    16261 cgggcattga ggagcagcgt cagccgcagg agagcgcgca gcagtatgtt gtgcgtctgg
    16321 cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca ggatctcccg gtgctgggtg
    16381 cggatactat cgttatcctg aatggagaag tgctggagaa accgcgcgac gcagagcatg
    16441 cggcgcagat gttgcgcaaa ttatcgggtc agacccatca ggtgatgacg gcagtggcgt
    16501 tggccgacag ccagcacatt ctcgattgcc tggtggtcac cgatgtgact ttcagaacgt
    16561 taacagacga agacatcgcg ggctatgtcg ccagcggtga accgttagat aaagcaggtg
    16621 catacggtat tcaggggctg ggtggctgtt ttgtcaggaa gataaatggc agctatcacg
    16681 ccgtagtcgg cttaccgctg gttgaaacgt atgaattatt aagtaatttt aacgcactgc
    16741 gtgagaaaag ggataaacat gacggctgaa ttgttagtaa acgtaacgcc ttcggaaacg
    16801 cgagtggcgt atattgatgg cggtattctg caggaaattc atattgaacg tgaggcgcga
    16861 cgcggaatag taggcaatat ctacaagggt cgtgtaagtc gtgtacttcc gggtatgcag
    16921 gcggcttttg tagatattgg gctggataaa gccgcgtttc ttcatgcatc cgatatcatg
    16981 ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat tcacggtgcg cgacatctcg
    17041 gaactggttc gtcaggggca agatctgatg gtgcaggtgg tgaaagatcc gcttggcact
    17101 aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc gctatctggt gtttatgcca
    17161 ggggcttctc acgttggggt ttcccaacgt attgaaagcg aatcagaacg tgaacgcctg
    17221 aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt ttatcatccg taccgcagcg
    17281 gaaggggttg gcgaggctga actggcctcc gatgccgctt atctgaaacg cgtctggacc
    17341 aaagttatgg agcgtaaaaa acgcccgcag acccgttatc agctgtacgg cgaactggcg
    17401 ctggcgcagc gtgttctgcg tgatttcgcc gacgccgaac tggaccgcat tcgcgttgac
    17461 tcacgcctga cttacgaagc attgctggag tttacctcgg agtacattcc cgagatgacc
    17521 agcaagctgg agcattacac cggacgccag ccgattttcg atctctttga tgtcgaaaac
    17581 gaaatccagc gagcgctgga acgcaaagta gaactgaaat ccggtggtta tctgattatc
    17641 gaccagaccg aagcgatgac caccgtggac atcaataccg gagcgtttgt cggtcatcgc
    17701 aatctggacg acaccatttt caataccaat attgaagcga cgcaggctat cgctcgccag
    17761 ttacggttgc gtaatctggg tgggattatc attattgatt tcatcgatat gaataatgaa
    17821 gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt tgagcaaaga ccgggtgaaa
    17881 accagcgtta atggtttttc ggcgctgggg ctggtggaga tgacgcgtaa acgcacccgc
    17941 gaaagcattg agcacgtact gtgtaacgaa tgcccaacct gccacggtcg cggaacggtg
    18001 aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga ttgttcgtgt ccaccatgct
    18061 tacgactccg accgtttcct ggtctatgca tctccggcag tagctgaagc cttgaaaggc
    18121 gaagagtcac actcgctggc ggaagtggaa attttcgttg gcaaacaggt taaagtacaa
    18181 attgaaccgc tctataacca ggagcagttt gacgtcgtaa tgatgtaaac agatgctggg
    18241 ccgccatccg gcaaagggtt tttgagtcac atttttagca gacaaggagt gacgggtgag
    18301 gcgattgccg gggattttac tgcttactgg agccgcgctc gttgtgatcg ctgctctgct
    18361 ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc tggcgtccgg aaatcctcaa
    18421 caaaatagaa tccgcgactg gcatgccggt agaagccagt cagctctcag ccagctggca
    18481 gaattttggc ccgacgcttg aagcacacga catccgtgca gaactaaaag atggcggcga
    18541 attttcggtt aaacgcgtta ctctggcgct ggatgtctgg cagagcctgt tacatatgcg
    18601 ctggcagttt cgcgacctca ctttctggca gctgcgcttt cgcaccaaca ctcctatcac
    18661 cagcggtggt agcgatgaca gtctggaagc cagtcacatc agcgatctgt ttcttcgtca
    18721 atttgaccat ttcgatctcc gcgacagtga agtcagtttt ctgacgccat ccggtcagcg
    18781 cgccgagctg gcgatcccac aactcacctg gctgaacgat ccacgtcgac accgtgcgga
    18841 aggcctggta agcctctcca gccttaccgg acagcacggc gtgatgcagg tgcgcatgga
    18901 tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc tggctacagg cggatgacat
    18961 cgacctgaag ccgtggctcg gtaaatggat gcaggacaat attgctctgg aaacggcaca
    19021 gttctccctt gaaggctgga tgacgatcga caaaggcgat gtaaccggcg gtgacgtctg
    19081 gctgaaacag ggcggtgcca gctggttggg cgagaagcaa acgcatacgc tgtcggtgga
    19141 taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg cagttctcta ttcccgatac
    19201 acggatcacg atggacggca aaccctggcc gagcggagca ttgacgctgg cctggatacc
    19261 ggaacaggac gttggcggca aagacaataa acgcagtgac gaactccgga ttcgcgccag
    19321 taatctggag ctggcaggcc tggagggcat acgcccgctg gccgcgaaac tttcacctgc
    19381 actgggtgat gtttggcgct ccacacaacc gagcggcaag attaacactc tggcgctgga
    19441 tatcccgctt caggcggcag acaagacccg ttttcaggca tcgtggagcg atctggcctg
    19501 gaagcaatgg aaattattac cgggtgcgga acacttctcc gggacgcttt ccggcagcgt
    19561 tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag atgccttacg aaacggtatt
    19621 ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata agctggctga acaatgacaa
    19681 aggtttccag ctggatgggc gtaatattga cgttaaagcc aaagccgtcc atgcgcgcgg
    19741 cggttttcgt tacctgcaac ctgctaacga tgaaccctgg ctgggtattc tggctggcat
    19801 cagtaccgat gatggttcac aagcctggcg ctatttcccg gaaaacttga tgggtaaaga
    19861 cctggttgat tacttaagtg gcgcgattca gggcggtgaa gcggataacg cgacgctggt
    19921 ttatggtggc aatccgcaac tcttccccta taaacacaac gaaggtcagt ttgaagtgct
    19981 ggtgccgctg cgcaacgcga agtttgcctt ccagccggac tggcctgcat taactaacct
    20041 tgatattgaa ctggacttta ttaacgacgg tttatggatg aaaaccgatg gcgttaatct
    20101 gggcggcgtg cgcgcgagta atctcaccgc agtgatccct gactactcga aagaaaaact
    20161 gctgattgac gctgacatta aaggtccggg taaagccgtt ggcccttact ttgatgagac
    20221 accgctgaaa gattctctgg gtgcgaccct gcaagaactc cagctcgacg gcgatgtgaa
    20281 tgctcgctta catcttgata tcccgctgaa cggcgaactg gtaaccgcga aaggtgaagt
    20341 gacgctgcgt aataacagtc tgtttatcaa accactcgac agcaccctga aaaatttgag
    20401 cggtaaattc agctttatca atggcgatct gcaaagtgaa ccactgacag caagctggtt
    20461 taatcagccg ttgaacgtgg atttttccac caaagaaggg gcaaaagcct accaggttgc
    20521 ggtgaatctc aacggtaact ggcaaccggc gaaaaccagc gttctgcctg aagcggtgaa
    20581 cgaagcattg agtggcagcg tggcgtggga tggtaaagtg ggcattgatc tgccttatca
    20641 tgctggcgcg acctataacg tagagctgaa cggcgatctg aataatgtga gtagtcactt
    20701 accttcaccg ttagccaaac ctgcgggtga accactagcg gtaaacgtta aggttgatgg
    20761 caatctcaac agctttgaat taaccggaca ggctggtgcg gataaccatt tcaatagccg
    20821 ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt tgggcggcag acagtaaaac
    20881 gctcccgccg ttgccggaac aaagtggcgt tgaactcaat atgccgccga tgaatggtgc
    20941 cgagtggctg gccctgttcc agaaaggcgc tgcggagagt gtcggtggtg cagcgagttt
    21001 cccacaacac ataacgttac gtacgcctat gttgtcactg ggaaatcagc aatggaataa
    21061 cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg gttgaggcgc aagggcgtga
    21121 aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg ctggcgaata tcaaatatct
    21181 ttattacaac ccgagcgtgg cgaaaactcg tggtgattca acgccgtcat cacctttccc
    21241 gacaacggag cgcattaact tccgtggctg gccggacgca caaatacgat gcacagagtg
    21301 ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat atcaccattt ctggcgatac
    21361 gttaacgctg accaatggac tgattgatac tggtttctcg cggcttactg ccgatggtga
    21421 atgggttaat aatccggaga atgaacgtac ctcgctgaaa ggaaaactgc gcgggcagaa
    21481 aattgatgcc gccgcagaat tttttggtgt cacgacgccc atacgccagt cgtcatttaa
    21541 tgtggattac gatttacact ggcgcaaagc accctggcaa ccagatgagg cgacgttgaa
    21601 tggcatcatt catactcaac tgggtaaagg cgaaattacc gaaatcaata ccggacatgc
    21661 cgggcaattg ctgcgcttat tgagcgttga tgccctgatg cgtaagctgc gttttgattt
    21721 cagagacact tttggcgaag ggttctattt tgactccatt cgcagcaccg cgtggattaa
    21781 agacggggtt atgcacaccg acgacacgct ggtggatggc ctggaggcgg atatcgccat
    21841 gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg gaagcggttg tcgcaccaga
    21901 gatttctgcg acggtgggcg tggctgcggc ttttgcggtt aaccccattg ttggcgcggc
    21961 agtatttgca gccagtaaag tgctggggcc gctgtggagc aaagtctcca ttttgcgcta
    22021 tcacatttcg ggtccgctgg acgatccgca aatcaacgaa gtgttgcgcc aaccgcgtaa
    22081 agaaaaagcg caatgatttg acgagggcgc gtaattgccc caatctcata tgataatcgt
    22141 tgccaaaggc caacgagccg gaacataact gtaggccgga tagggcgttc acgccgcatc
    22201 cggcagccgt aaaaaatcct ctactgcagt aactaacgag tagcaaaaac gatgagtctg
    22261 aacctggtaa gtgaacaatt gctagcggcg aacggcctga aacatcagga cttgttcgcg
    22321 atcctcggtc aactggccga acgtcgcctt gattatggcg atctctattt tcagtcgagc
    22381 tatcacgaat cctgggtttt agaagaccgc attattaaag atggttctta caacatcgat
    22441 cagggcgttg gtgtgcgtgc aatcagtggt gaaaaaaccg gatttgctta cgctgaccaa
    22501 atcagcctgc tggcgctgga acagagtgcg caagcggcgc gcaccatcgt ccgtgatagt
    22561 ggtgatggca aagtacagac gctgggcgcg gtagagcata gcccgttgta tacctcggta
    22621 gatccgctgc aaagcatgag ccgtgaagag aagctggata tcctgcgtcg cgtcgataag
    22681 gttgcccgcg aagcggacaa gcgcgtacaa gaagtgactg ccagcctcag cggcgtttat
    22741 gaattaattc tggttgcggc caccgacggc acgctggcgg cggatgttcg tccgctggtg
    22801 cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac gcgaacgcgg tgccagtggc
    22861 ggcggcggtc gttttggtta tgagttcttc cttgccgatc ttgacggcga agtccgtgcg
    22921 gatgcatggg caaaagaagc agtgcgtatg gcgctggtca atctttctgc cgttgctgca
    22981 ccagcgggca ccatgccggt agtacttggc gcaggttggc cgggcgtgct gttgcatgaa
    23041 gcggtaggtc acggtctgga aggcgacttc aactgccgcg gcacttcagt atttagtggg
    23101 caggtcgggg agctggtggc ttcagaactg tgtaccgtgg ttgatgacgg cacgatggtc
    23161 gatcgccgtg gttcggtggc gattgatgac gaaggtacgc caggccagta caacgtgctg
    23221 attgagaacg gcattctgaa aggctacatg caggataaac tcaacgcgcg tttgatgggg
    23281 atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc atctgcccat gccgcgtatg
    23341 accaacacct atatgctgcc gggtaaatcg accccgcagg aaattattga atccgttgag
    23401 tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg atattacatc cggcaaattc
    23461 gttttctcca cttcagaagc gtatctgatt gaaaacggta aagtaacgaa gccggtgaaa
    23521 ggcgcgacgt tgattggttc cggtatcgaa accatgcagc agatttcgat ggttggcaac
    23581 gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag aagggcaaag tttgccggtt
    23641 ggcgtgggcc agccaacgct gaaagttgat aacctgactg ttggcggtac tgcgtaataa
    23701 tttcattatt ttcagaagga taattaatct ttctacgtgc acgaacggtc ccctcgctcc
    23761 tctggggtta gggtgagggg aacccgttgg cacaggtttg tagaacgtaa cagtacaata
    23821 tgaattactt ctctttcccg cgcccgtgca tctcctgaaa caatttaccg acctcaacaa
    23881 aataatccgt cagcgagttg atcacgacct gtaccttcag cggcagctta tctttttcgg
    23941 tatataacgc ataaaccggg cgtggatctg actgataacg cggcagcagg atctccagct
    24001 caccgcgatt gatctcgttg atcacccaca tcagcggcac gtaggcgatc ccggcacccg
    24061 ccgttagcca gcgcaccagc gtcatcggat cattagtcac aaatctccct tgcgggatca
    24121 ggcgagtcga gatcccttcc ggtgcaatca gttcaaattc attgtcgggc cgcacgctgt
    24181 attcaagcca tgaatggcta cttaaatcgg cgggtttttc cggtatgccg tattgtgtga
    24241 gatagctttt cgcggcgcac accaccattg gcatcgcgcc aagacggcgg gaaaacaggc
    24301 tggaatcctg caacgcgccg acgcggatca ccacatccag accgtcggca atcaggtcgg
    24361 gggctggaat tccggtaacc agattgacgc tcaaacctgg gtattctttc agcattttgg
    24421 ctgtcagccc ggcgagaaca ttttgtgcca tagttgaaga acagccaatg cgtagcgtcc
    24481 cgatgggggt gttattgaag gcatacagtt gctcatgaac atcctgcact tcatgaagca
    24541 tacgacggca gccctggtag taaattctac cggcttcggt caggccaatg ctgcgtgtgc
    24601 tacggtttaa cagctttacc tgcaactcat cttccagttt tgacaccgtc tgactgatgg
    24661 acgaaacgct catctgtagc tgtctggcgg cggcggtaaa agagccaaat tcaactactt
    24721 tggcaaacac cgacatgcgt tttagtcgtt ccattattca ctctgactta aaagtgattt
    24781 agatcacata atatagataa cagcataaca gttacgctaa tatattaaat atcaatctac
    24841 agcaatgttg ctctcgcccg gctttccatg cccttctctg cggcgacaga tgctgaaaat
    24901 aataacgcct gctctctcta tacaaccaag gtcaacatga gtctgtttcc cgttatcgtg
    24961 gtgtttgggc tgtccttccc accgatattt tttgaattgc ttttatcact ggcgattttc
    25021 tggctggtgc gccgggtact tgtgccaaca ggtatctacg actttgtctg gcatccggcg
    25081 ttgttcaaca ccgcgctcta ttgctgcttg ttttatttga tatcgcgact gttcgtttga
    25141 ggttgaagtg aaaacactaa taagaaaatt ctcccgtacg gccatcacgg tcgtattagt
    25201 cattctggcc ttcatcgcaa tttttaatgc ctgggtctat tacaccgaat ccccctggac
    25261 gcgtgacgcg cgctttagcg ctgacgtcgt tgcgatcgcg ccggacgttt ctggactcat
    25321 tacccaggtg aatgttcatg ataaccagct ggtgaaaaaa ggacagatac tgttcaccat
    25381 cgaccagccg cgctatcaaa aggcgcttga ggaagcgcaa gccgatgttg cctattatca
    25441 ggtactggca caggagaaac gccaggaggc cggacgtcgt aaccgtctcg gtgtgcaggc
    25501 gatgtctcgc gaagagatcg accaggctaa caacgtacta caaacggttc tgcatcagtt
    25561 agcgaaagcg caggcgaccc gcgatctggc aaaactggat cttgaacgca cggtgatccg
    25621 cgcgccagca gatggctggg tgaccaacct caacgtctat accggtgagt ttattactcg
    25681 aggatcaacg gcggttgcgc tggtgaaaca gaactccttc tatgtactgg cctatatgga
    25741 agaaactaag ctggaagggg tgcgtccggg gtatcgtgca gagatcacgc cgcttggcag
    25801 taacaaagtg ctgaaaggga ctgttgatag tgttgccgct ggggtcacca acgccagcag
    25861 cacgcgtgac gacaaaggga tggcgaccat cgactctaac cttgaatggg tgcgtttggc
    25921 gcaacgtgtt ccggttcgta ttcgtctcga caaccagcaa gagaacatct ggcctgcggg
    25981 caccactgct acagtggtgg tcactggcaa acaagatcgc gacgaaagcc aggattcgtt
    26041 cttccgtaaa atggcccatc gcctgcgtga gtttggttaa tcacgatggg tattttctcc
    26101 attgctaacc aacatattcg ctttgcggta aaactggcga ccgccattgt actggcgctg
    26161 tttgttggct ttcacttcca gctggaaacg ccacgctggg cggtactgac agcggcgatt
    26221 gttgccgccg gtccggcctt tgctgcggga ggtgaaccgt attctggcgc tattcgctat
    26281 cgtggctttt tgcgcatcat cggcacattt attggctgta ttgccggact ggtgatcatc
    26341 attgcgatga tccgcgcacc attattgatg attctggtgt gctgtatctg ggccggtttt
    26401 tgtacctgga tatcctcgct ggtacgaata gaaaactcgt acgcgtgggg gctggccggt
    26461 tataccgcgc tgatcattgt gatcaccatt cagccggaac cattgcttac gccgcagttt
    26521 gccgtcgaac gttgtagcga gatcgttatc ggtattgtgt gtgcgattat ggcggatttg
    26581 ctcttttctc cgcgatcgat caaacaagaa gtggatcgag agctggaaag tttgctggtc
    26641 gcgcaatatc aattaatgca actctgtatc aagcatggcg atggtgaagt tgtcgataaa
    26701 gcctggggcg acctggtgcg acgcaccacg gcgctacaaa gcatgcgcag caacctgaat
    26761 atggaatctt cccgctgggc gcgggccaat cgacgtttaa aagcgatcaa tacgctatcg
    26821 ctgacgctga ttacccaatc ctgcgaaact tatcttattc agaatacgcg cccggaattg
    26881 atcactgata ctttccgcga attttttgac acgccggtag aaaccgcgca ggacgtccac
    26941 aagcagctca aacgcctgcg gagagttatc gcctggaccg gggaacggga aacgcctgtc
    27001 accatttata gctgggtcgc ggcggcaacg cgttatcagc ttctcaagcg cggcgttatc
    27061 agtaacacaa aaatcaacgc caccgaagaa gagatcctgc aaggcgaacc ggaagtcaaa
    27121 gtagagtcag ccgaacgtca tcatgcgatg gttaacttct ggcgaaccac actttcctgc
    27181 attctgggca cgcttttctg gctgtggacg ggctggactt ccggcagtgg tgcaatggtg
    27241 atgattgcgg tcgtgacgtc actggcaatg cgtttgccga atccacgcat ggtggcgatc
    27301 gactttatct acgggacgct ggccgcgctg ccgttagggc tgctctactt tttggtgatt
    27361 atccctaata cccaacagag catgttgctg ctgtgtatta gcctggcagt gctgggattt
    27421 ttcctcggta tagaagtaca gaaacggcga ctgggctcga tgggggcgct ggccagcacc
    27481 ataaatatta tcgtgctgga taacccgatg actttccatt tcagtcagtt tctcgacagc
    27541 gcattagggc aaatcgtcgg ctgtgtgctc gcgttcaccg ttattttgct tgtgcgggat
    27601 aaatcgcgcg acaggaccgg acgtgtactg cttaatcagt ttgtttctgc cgctgtttcc
    27661 gcgatgacta ccaatgtggc acgtcgtaaa gagaaccacc tcccggcact ttatcagcag
    27721 ctgtttttgc tgatgaataa gttcccaggg gatttgccga aatttcgcct ggcgctgacg
    27781 atgattatcg cgcaccagcg cctgcgtgat gcgccgatcc cggttaacga ggatttatcg
    27841 gcgtttcacc gacaaatgcg ccgcacagca gaccatgtga tatctgcccg tagcgatgat
    27901 aaacgtcgtc ggtactttgg ccagttgctg gaagaactgg aaatctacca ggaaaagcta
    27961 cgcatctggc aagcgccacc gcaggtgacg gaaccggttc atcggctggc ggggatgctc
    28021 cataagtatc aacatgcgtt gaccgatagt taagtcaaaa ccgacgccaa aagcgtcggt
    28081 tttttcatgg ctatacttag cgatgcacgg cagaactcgc cgcgaaacgt gacggtggca
    28141 acagatgaat atttatacct ttgattttga tgagattgag agtcaggagg atttttatcg
    28201 tgactttagc caaacctttg gtctggctaa agataaggta cgcgatctcg actcactatg
    28261 ggatgtgtta atgaacgatg tcctgccgct accacttgag attgaatttg ttcatctggg
    28321 agagaaaacg cgtcgccgtt ttggcgcgtt aatattgctg tttgatgagg cagaggaaga
    28381 gctggaaggg catttgcgtt ttaatgttcg tcattagcgc aaaaaaaagc ccccgaaccg
    28441 ggggcaatat cgtcggacaa gacgatgagg gtttatttgt acagttcagc cgtagcgtgc
    28501 caggtgtcac cgctacgagc ttcagtaatc tggtaggccg ttgcgccttt ctcttccgct
    28561 tttttgttca gcatttcacg catatccatt ggcgaagacg ccacaccact tacggatacg
    28621 gtcccgattg cttcacgatt ttgtgcttgt gcagcatcaa tggagtcggc agcgaatgca
    28681 ccgaaagaga gaacagaaag tacgcttaat gcagctacag tggttttgat tttcatgatt
    28741 tttacctcga cataatcttt tagctgggcc tttgtttcgt gacccatatc acaaaatcaa
    28801 gtatacacta attattgagc taattaatac cacgctaact gtttcagttg atactaagtg
    28861 ttaaaaatgt gaattttaat a
//
LOCUS       DAZFJF010000058        28304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFJF010000058 DAZFJF010000000
VERSION     DAZFJF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.058"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHKAU_002669"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHKAU_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO5531700.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHKAU_002670"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHKAU_002670"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO5531701.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHKAU_002671"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHKAU_002671"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO5531702.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHKAU_002672"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHKAU_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO5531703.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHKAU_002673"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHKAU_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO5531704.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHKAU_002674"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHKAU_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO5531705.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHKAU_002675"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHKAU_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO5531706.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHKAU_002676"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHKAU_002676"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO5531707.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHKAU_002677"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHKAU_002677"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO5531708.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHKAU_002678"
     CDS             9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHKAU_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO5531709.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHKAU_002679"
     CDS             10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHKAU_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO5531710.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHKAU_002680"
     CDS             10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHKAU_002680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO5531711.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHKAU_002681"
     CDS             12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHKAU_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5531712.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHKAU_002682"
     CDS             complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHKAU_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO5531713.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHKAU_002683"
     CDS             complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHKAU_002683"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO5531714.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHKAU_002684"
     CDS             complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHKAU_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO5531715.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHKAU_002685"
     CDS             complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHKAU_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO5531716.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHKAU_002686"
     CDS             complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHKAU_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO5531717.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHKAU_002687"
     CDS             16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHKAU_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO5531718.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHKAU_002688"
     CDS             17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHKAU_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO5531719.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHKAU_002689"
     CDS             complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHKAU_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO5531720.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19444..20322
                     /gene="nadK"
                     /locus_tag="ACHKAU_002690"
     CDS             19444..20322
                     /gene="nadK"
                     /locus_tag="ACHKAU_002690"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO5531721.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20408..22069
                     /gene="recN"
                     /locus_tag="ACHKAU_002691"
     CDS             20408..22069
                     /gene="recN"
                     /locus_tag="ACHKAU_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO5531722.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22218..22559
                     /gene="bamE"
                     /locus_tag="ACHKAU_002692"
     CDS             22218..22559
                     /gene="bamE"
                     /locus_tag="ACHKAU_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO5531723.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHKAU_002693"
     CDS             complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHKAU_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO5531724.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHKAU_002694"
     CDS             complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHKAU_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO5531725.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23509..23991
                     /gene="smpB"
                     /locus_tag="ACHKAU_002695"
     CDS             23509..23991
                     /gene="smpB"
                     /locus_tag="ACHKAU_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO5531726.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHKAU_002696"
     tmRNA           24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHKAU_002696"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24839..25087
                     /locus_tag="ACHKAU_002697"
                     /pseudo
     CDS             24839..25087
                     /locus_tag="ACHKAU_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            complement(25196..25353)
                     /locus_tag="ACHKAU_002698"
                     /pseudo
     CDS             complement(25196..25353)
                     /locus_tag="ACHKAU_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            25682..26332
                     /gene="nleG7"
                     /locus_tag="ACHKAU_002699"
     CDS             25682..26332
                     /gene="nleG7"
                     /locus_tag="ACHKAU_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO5531727.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQKQHCK"
     gene            complement(26557..27432)
                     /locus_tag="ACHKAU_002700"
     CDS             complement(26557..27432)
                     /locus_tag="ACHKAU_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO5531728.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
     gene            complement(27573..27842)
                     /locus_tag="ACHKAU_002701"
     CDS             complement(27573..27842)
                     /locus_tag="ACHKAU_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5531729.1"
                     /translation="MNILKKLMQCLCGCGKHDGREHVQSPTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(27844..>28304)
                     /locus_tag="ACHKAU_002702"
     CDS             complement(27844..>28304)
                     /locus_tag="ACHKAU_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5531730.1"
                     /translation="RGPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAG
                     PAGPQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPASIIETNSNGWFPDTDGA
                     LITGLTFLDPKDATQVQGLFRHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT         7275 a         7394 c         6496 g         7139 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa agcatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagca ttgatggcaa ccagctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ctattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
    10381 tgttttattt ctcacattga tgacgatcgc atgaaaaaat gatattttaa tgagcattct
    10441 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
    10501 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaggagcc
    10561 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
    10621 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
    10681 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
    10741 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
    10801 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
    10861 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
    10921 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
    10981 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
    11041 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
    11101 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
    11161 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
    11221 caactgaaac actggcagcg ctgggccagc aagggcaatt ttcaactgca gaagtacgtg
    11281 ataagcagca aaatattctg gcgtcctggc attacacccg taaggatcca ggcgatactt
    11341 tcagcaattt cataagccac tggctcttcc ccgcccccat cattcagccg attcgtcaca
    11401 atggtgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
    11461 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcaggc atcgcaatta
    11521 ccctcacccg ccatttgcac aatggcctgg tagaagcgct gaaaaatatc accgatgtcg
    11581 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg
    11641 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
    11701 ttcgtttaca ggccaaaaat gcgcagcttc tgcgtaccgc gctacatgac ccattaaccg
    11761 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaatac gttgatgaac aattccgatg
    11821 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatat atcaatgata
    11881 cctggggtca tgcgacgggc gatagagtct tgattgaaat cgcaaaacgg ttagctgaat
    11941 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
    12001 atgatgtaca gtcggaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
    12061 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
    12121 cgatgaccat tgagcacgcc tctgcgaaaa aattacaaga gcttgccgat cacaatatgt
    12181 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
    12241 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
    12301 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
    12361 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
    12421 aattgctccc ggaaagccag caacagatcc aaaccatggc agccaaattg gcctcgacag
    12481 ggctaacaca tgcccgtatg gatggacaca ccgataacta tggtgaagac agttacaacg
    12541 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa
    12601 ttccgcgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
    12661 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattact accccttaaa
    12721 acaccagcaa acagataaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
    12781 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
    12841 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
    12901 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
    12961 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
    13021 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
    13081 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
    13141 cataatttaa tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca
    13201 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
    13261 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
    13321 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact
    13381 tccatccctt ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
    13441 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
    13501 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
    13561 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
    13621 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
    13681 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
    13741 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
    13801 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccaact ctggatgctc
    13861 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
    13921 tcaggaaaca ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg
    13981 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
    14041 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
    14101 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcgac gactttaccg
    14161 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtcc
    14221 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
    14281 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
    14341 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga
    14401 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
    14461 ccgtaagacg aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt
    14521 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
    14581 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
    14641 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
    14701 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
    14761 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
    14821 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
    14881 tcatttttgc gggaattgca atcaacagtt gctaactctg ctgtaaaagg ccgtcggcgg
    14941 tgcagccagt ttggtgccgg agtgcgcgca gtcaccggag cgtacacgca gtacgtgagg
    15001 atgacgagca catcccggtg ccaaaatggc aaacaagcca ggccgattag cgaccaggga
    15061 agcctggggg catcataccc ttcatgcttc tcatcatctt cgccattccg cctttcttca
    15121 ttttcttcat catgcgctgc atgtcgtcga actgcttcag aaggcggttc acgtcctgca
    15181 cctgcatacc gcaaccggca gcgatacgac gcttacgcga acctttgatg atttctggct
    15241 tagcgcgctc ttttatcgtc atcgagttga tgatggcttc catacgcacc agcactttat
    15301 cgtccatctg cgacttaaca ttatccggga tctgccccat gcccggcagc ttgcccatca
    15361 ggctagccat gccgcccata tttttcatct ggcgcagttg ctcaagaaaa tcgttgagat
    15421 cgaagccgtc gccttttttc agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
    15481 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
    15541 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg agaaacttga
    15601 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
    15661 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
    15721 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
    15781 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
    15841 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacgatat
    15901 ctaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
    15961 tctcaagctg tttgattgcc gccgggcgat aaacgtcggc agaaaccacc agcactttct
    16021 tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac gctggttgtt ttaccggcac
    16081 cttgcaggcc cgccatcagt acgaccgcag gcggttgcgc agccaggttc agggtctggt
    16141 tctcttcgcc catcgccgca accagttcgt tacggactat tttgacgaac tcctgccccg
    16201 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
    16261 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
    16321 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
    16381 acaaacgatc ggttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
    16441 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
    16501 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
    16561 ctgtcgacat tactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
    16621 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
    16681 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
    16741 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttgatga
    16801 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
    16861 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
    16921 cccatctgga agcaacgcct gggatgctgg tgcacattgg cttatcgctc ttttcctatg
    16981 ccacgctgat aattgccgcc ctgtacgcgc tacaactggc gtggattgat taccaactga
    17041 agaacaagaa gctggcgttt aaccaagaaa tgccgccatt gatgagtatc gagcgtaaaa
    17101 tgttccacat cacgcagatt ggcgtggtgc tactaacgct cacgctttgc actggcctgt
    17161 tctacatgca caacttattt agcatggaaa atatcgacaa ggccgtgctc tctatcgtgg
    17221 cgtggtttgt ctatattgtg ctgctgtggg gacattatca tgaaggatgg cgtggacgcc
    17281 gcgtcgtctg gtttaacgtt gcgggtgcag tcatcctgac actggcctac ttcggcagcc
    17341 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
    17401 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
    17461 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctg cgacatatgg cgaaacaggg
    17521 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
    17581 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgttgg
    17641 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
    17701 actggttttt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
    17761 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
    17821 gctgaatgct atcacccgta tgctgatgcg catgatgggt atcaaaaccg atatcgtggt
    17881 tagcggctct ttgagcaaag aagagttgcg cactatcgtg cacgaatcac gctcacaaat
    17941 ttcccgtcgc aatcaggata tgctactgtc tgtgctcgat ctggaaaaga tgaccgttga
    18001 tgacatcatg gtgccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
    18061 gattctgcgc caactctccc actcacctca cgggcgcatc gtgctctacc gtgattcgct
    18121 ggacgacgcc atcagtatgc tgcgagtacg tgaagcctgg cggttgatgt cggagaaaaa
    18181 agagttcacc aaagaaacca tgctgcgcgc cgcggacgag atctattttg tgccggaagg
    18241 tacgccgctc agcacgcagt tggtaaagtt tcagcgcaac aaaaagaaag tcggcctggt
    18301 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
    18361 tgtcggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
    18421 cgacggttcg gtgattatcg atggcaccgc caacgtgcgg gaaatcaaca aagcctttaa
    18481 ctggcatcta ccggaagatg atgcccgcac ggttaatggc gtcattcttg aggcactgga
    18541 agagatccct gtcgcaggca cccgcgtgcg tattggcgag tacgatatag atattctcga
    18601 cgtacaggac aatatgatta agcaggtaaa agtttttcct gtgaaaccgc tgcgcgagag
    18661 tgtggcggag taacgaaaac ggccctgcat tcgcatgcag ggccgtgaat tattacgaaa
    18721 acagaaatta cgcttttgct ttcgctacag ttaccatcgc cgcacgaatc gtacgaccat
    18781 tcagcgtata acccttctgc ataatgccca gtacgttacc tggcgcaacg tcatcagatt
    18841 ccaccattgc gatagcctga tgcacattcg ggtccagcgg cacgttagtt tcggcgatca
    18901 cttcaacacc aaacttacgc acaacatcca gcatcgactt cagcgtcagc tcaatgcctt
    18961 caaccatcgc agacatatcc gggttagctt tatcagccac ttccagcgca cgatccaggc
    19021 tatcaatcac cggcagcaat tcgttgatga atttctccag cgcgaatttg tgggcttttt
    19081 caatatccag ttcagtacgt cgacgcaggt tttccatttc ggcttttaca cgcaaaatgc
    19141 cgtcacgttc acgggtctgg gcttcagcca gctgagcttc gagattcgca attttttcat
    19201 cgcgcggatc cacctgctca gcagaagctt ctggctcaac tgcctcaatc tcttcgtgct
    19261 gatccatgat aatttcttcc ggggcttgcc cctcaggcgt tttctgttct ttactactca
    19321 tgaatttctc cgcgtttttt tcgcattcat ctcgctaact tcgcttatta tggggatcag
    19381 tttcagggtt tcaagggaag cactcacatt gtcatcaatc ttcgcaacaa ggacctcgga
    19441 aaaatgaata atcatttcaa gtgtattggc attgtgggac acccacggca ccccactgca
    19501 ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa aaggttacga ggtcatcgtt
    19561 gagcaacaaa tcgctcacga actgcaactg aagaatgtga aaactggcac gctcgcggag
    19621 attgggcaac tagctgatct cgcggtagtc gttggtggcg acggtaatat gctgggcgcg
    19681 gcacgcacac tcgcccgtta cgatattaaa gttattggaa tcaaccgtgg caacctgggt
    19741 ttcctgactg accttgaccc cgataacgcc cagcaacagt tagccgatgt gctggaaggc
    19801 cactacatca gcgagaaacg ttttttgctg gaagcgcaag tctgtcagca agattgccag
    19861 aaacgcatca gcaccgcgat aaatgaagtg gtgcttcatc caggcaaagt ggcgcatatg
    19921 attgagttcg aagtgtatat cgacgagatc tttgcgtttt ctcagcgatc tgatggacta
    19981 attatttcga cgccaacagg ctccaccgcc tattccctct ctgcaggcgg tcctattctg
    20041 accccctctc tggatgcgat taccctggtg cccatgttcc cgcatacgtt gtcagcacga
    20101 ccactggtca taaacagcag cagcacgatc cgtctgcgtt tttcgcatcg ccgtaacgac
    20161 ctggaaatca gttgcgacag ccagatagca ctgccgattc aggaaggtga agatgtcctg
    20221 attcgtcgct gtgattacca tctgaatctg attcatccga aagattacag ttatttcaac
    20281 acattaagca ccaagctcgg ctggtcaaaa aaattattct aattttacgc cagcctcttt
    20341 actgtatata aaaccagttt atactgtaca caataacagt aatggttttt catacaggaa
    20401 aacgactatg ttggcacaac tgaccatcag caactttgct atcgttcgtg agcttgagat
    20461 tgattttcat agcggcatga ccgtaataac tggcgagacc ggcgcgggta aatctattgc
    20521 aatagatgcc ctcggtcttt gtctcggtgg tcgcgctgaa gccgacatgg tgcgtaccgg
    20581 cgctgctcgc gctgacctgt gcgcccgttt ttctctgaaa gatacgccag cggctctgcg
    20641 ctggctggaa gaaaatcagc ttgaagacgg gcatgaatgt ttgcttcgtc gagtgatcag
    20701 cagcgatggt cgctcccgtg gtttcatcaa cggtacagct gttcctctgt cacaactgcg
    20761 cgaactgggt cagttgctga ttcagatcca tggtcagcac gctcatcaat tactcaccaa
    20821 acctgagcac caaaaattcc tgcttgatgg ctatgccaat gaaacctctc tactgcagga
    20881 aatgaccgca cgttatcagt tgtggcatca aagctgccgt gacctcgcgc atcatcaaca
    20941 gttaagtcag gaacgcgccg cccgtgcgga actgctgcaa taccaattaa aagaacttaa
    21001 cgaatttaat ccgcagcccg gagagtttga acaaatcgac gaagagtaca aacgtctggc
    21061 gaacagcggt caattgctga ccaccagcca gaatgcattg gcattaatgg ccgacggtga
    21121 agacgcaaac ctgcaaagtc agctttacac ggctaaacaa ctggtgagcg aattgattgg
    21181 catggacagc aaactgtccg gcgtacttga tatgctggaa gaagctacca tccagattgc
    21241 tgaagccagc gatgaactgc gccactactg cgatcgtctg gatctcgatc ccaaccgact
    21301 atttgaactt gaacagcgca tctcaaaaca gatttcgctg gcacgtaaac atcacgtcag
    21361 ccctgaggca ttgccacagt attaccagtc gctactggaa gaacagcagc aactggacga
    21421 tcaggccgac tcacaagaaa cgcttgcgct ggcggtaacg aaacatcatc agcaggcact
    21481 ggaaatcgcg cgcgcattac accaacaacg ccagcaatat gcagaagaac ttgcacagct
    21541 gatcaccgac agtatgcatg cgctctcaat gccgcatggg cagtttacga tcgatgttaa
    21601 atttgacgag catcacctgg gcgctgacgg tgccgatcgt attgagtttc gggtaaccac
    21661 caacccaggt cagccaatgc agcctattgc caaagtcgca tccggtggtg aattgtcccg
    21721 catcgcactg gcaatccagg tcatcacggc gcgtaaaatg gaaaccccgg cactgatttt
    21781 tgatgaagtg gatgtaggga ttagcggtcc aacagcggca gttgtcggca aactgctgcg
    21841 tcaactcggc gaatcaactc aggtgatgtg tgttacccac ctgccacaag tcgcgggatg
    21901 tggtcatcaa cactattttg tcagcaaaga aaccgatggt gcgatgacag aaacgcatat
    21961 gcaatccctg aataaaaaag cgcggttaca agagctggcg cgcctgcttg gtggcagtga
    22021 agtcacacgt aatacactgg cgaatgcgaa agaactgctt gcagcgtaaa cttttttcct
    22081 gcttcacggt cagagtaaac agcaaaacgc cgtaagaccg gaaagcaaaa ggttttaaag
    22141 tgatgaaagg tctattatca tcggcatatt acagatgagc cacgtactgc tcgggcccga
    22201 aaaggaatca aatcactatg cgctgtaaaa cgctgactgc tgcagcagca gtactattga
    22261 tgttgaccgc aggctgttcc actctggagc gagtggttta ccgtcctgac atcaaccagg
    22321 ggaactatct gaccgctaac gacgtatcca aaatacgtgt tggcatgacg caacaacaag
    22381 ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt tggtacgaat acctggttct
    22441 atgtcttccg ccagcaacca ggtcatgaag gtgtaacgca gcaaacgctg acgctgacct
    22501 ttaacagtag cggtgtgttg accaatattg ataacaaacc tgcgctgagt ggtaactaat
    22561 aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat gagcaccttt tttctgtctg
    22621 ttatttattc gctgattttt ctgctcgttg cctgcgaagc tctttcggat cggcaatgag
    22681 agggcgataa atctccaccc gatcgccatc atgcacacta tcgcttagtt ttgccggacg
    22741 gctgtaaatg ccgactttgt ttttagttaa atcgatatcg gtacgcaatt ccagcaagcc
    22801 actggcgcga atagcttctt caaccgtcgc gccctcctgc agcgtcacgc gctgcaggta
    22861 ctgcttctca ggtagcgcat aagccacctc aacggcaatt ttacctggca ctgtaaacct
    22921 ctttcgcacg aaccgtaaaa gcctggacca tattagacgc cagctcttta aacacgcgac
    22981 caaaggcgag ttcaatcaac ttattggtaa actcaaagtc gagatgaaac tcgatacgac
    23041 acgcctcctg gctcagcggc gtaaacttcc atccaccaat caatttcttg aacggcccat
    23101 ccaccagatt cataagaata ctttggttac tggtcaactg gttgcgggta gtaaacgttt
    23161 tgctgatccc agccttagag acatctaccg ccgcagtcat ctgcccagga gtggactcca
    23221 gaatccgact tccggtacaa cccggcaaaa actgaggata agactgaacg tcattcacta
    23281 actgatacat ttgctccgcg ctgtagggta ccagtgcggt ccggctaatc tgaggcataa
    23341 caatttccat caacaaaaat ccaacaaata atatcattta tcctgctgta aaaaaaacgc
    23401 tatcccggcg ctgggtaaca tcgggttcat gctaagatag agccttgtcc cccgcaggat
    23461 tgatatgggg tgttttcgat ttcagattac cgatgattca cgacgcttat gacgaagaaa
    23521 aaagcacata aacctggttc agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac
    23581 tttatcgaag aagagttcga agcgggactt gccctgcaag gctgggaggt taaatccctg
    23641 cgcgcaggaa aagccaatat cagcgacagc tacgtccttc tgcgtgacgg agaggcattt
    23701 ctgtttggcg ctaacatcac gccaatggcc gtggcctcca cgcatgtggt gtgcgatcct
    23761 acccgtaccc gcaagttact tctcaaccag cgcgaactgg actcattgta cggtcgcgtc
    23821 aatcgagaag gctataccgt agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa
    23881 gtgaaaatcg gcgtcgccaa aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa
    23941 cgcgaatggc aggtggataa agcacgtatc atgaaaaacg cccaccgtta aacctgcact
    24001 ccaattattg accagttcct caccgcgcct ccctctccgg cggcgcgaat gaacatctta
    24061 ttggctatca catccgacac aaatgttgcc atcccattgc ttaatcgaat aaaaatcagg
    24121 ctacatgggt gctaaatctt taacgataac gccattgagg ctggtcatgg cgctcataaa
    24181 tctggtatac ttacctttac acattggggc tgattctgga ttcgacggga tttgcgaaac
    24241 ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac
    24301 gacgaaaact acgctttagc agcttaataa cctgcttaga gccctctctc cctagcctcc
    24361 gctcttagga cggggatcaa gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc
    24421 tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc
    24481 tggcaagcga atgtaaagac tgactaagca tgtagtaccg aggatgtagg aatttcggac
    24541 gcgggttcaa ctcccgccag ctccaccact tttgatagga ctgcaatcgg acagcggcaa
    24601 taaaaacagc cacttacgga ctctgaccag acagagcgca gaccgagaaa agacaaaaaa
    24661 atgcacgtaa aatgcacgtg cactttaaaa gaaccccaga tctcacggtc tggttttttt
    24721 ctgtttgtaa ctaatggtaa caaaaacaca tcacctcttg gcgttccttc tccttgatac
    24781 tgtttatcca tacagttaaa aataacactg tatacaaaca cagtatagag agatttttat
    24841 gcgtattgaa atttgcatag ccaaagaaaa aatcagcaaa atgccaaaag gatctgttcc
    24901 tgccctacag caagaaatgc tgtgacgtgc cagtaaacgt tatgacgatg tagaagtgat
    24961 catcaaatcc accagcaacg atggcctttc agttactctc accgccgata aagattctgc
    25021 aaaaactttt gttcaggaga cgctgaaaga tacctgggag tctgctgacg agtggtttgt
    25081 tcgctaatta acgagtaaaa tcagtaacgg ctggaaatca ttcaatactc acactatcta
    25141 aaagttcacc agccaacagc ggtaagttct ttcatacgac gtgccgcgga tcctattatt
    25201 ctagtatgaa atccctaacc gatttcaaat tctccagact gctcagattc tcttttatcc
    25261 cacgctggcg ctgataaatc tcgtcattgc aatcgacctg cgctatcgct gccgcagttc
    25321 ttccagtttc tgcatcgata actccatctt ctgagcaata aacctcacct atgcaatatt
    25381 ttatgtcaat tatttcaatt gcattaatat cttgaatgga ttacatagag ttaatgtatc
    25441 cagtactccc tattctcttg catataatac atgttgcaac ttacatctca gcgctatgaa
    25501 aaaacaccac ctctctcttt atgaaattct ggatttacca agcgctaatt tatcatttca
    25561 atctaccttt aagcattgca tttatctccc tacgaggtca tactttagga agttaaatat
    25621 gaatgataat atacctacag cgcgaaatca caaacaatcg acttgtatta cagaaaaaaa
    25681 catgcctatc tttttaaact tcacagcagg cagtatctta cctgagaatg agctagcatc
    25741 tttacgttat attgtgcagc aaaatcaaaa tgatactgta atcataaaag aacgttataa
    25801 aatggatatc cgttatatcg aatcagtcaa tggttttaca gtaaatcctg tatgcagtaa
    25861 tcatttctcc atatttatgg cgagacaaaa cactattgct cgcaacctgg aacagcagat
    25921 caacaacgga cgaagttttg cacaaatatc tcaggatttt atgcttcaat tatcttcaaa
    25981 tataggatgg aaaaaagggg ccgaaaacgc ccttaaaaat aaaatccatt ctcattcatt
    26041 tgttgtaaat cctgatgaat tctcttgcga cacacaattt cttaagtgcc caataacatt
    26101 atgcgttcca gaaaaagggg tttttgtcaa gaacgcactg aactccaaca tatgcactct
    26161 ttatgataag tctgcgttca tgaatctcac aagagaacat ctaccccacc ctctcagcag
    26221 ggaaaagata gtaaaagaaa tgattattga aaggaatatg tgttattttg acaccataag
    26281 tcagcatttc ataattatgg atgcagacca acagaaacag cattgtaaat aaaatgtaat
    26341 aattacatac tattagtgat tctcatgcat cgtaagcggc tcgccagaac cgtattgata
    26401 tttactgagc gctcagatca actttccatg gcaacagatc gcgtacccgg tttgccggcc
    26461 agtcctggat atgctcccgc cgacaatatt aatggccagg aaatgtttaa aacaatgatt
    26521 ctgtttccta accatcaagt gtatatgctg gctcaattac tcaatatcta tgggttgagt
    26581 tctacccaaa gtggctctgt agtcgcttct tcttacagta tgttcaggtg ttgcaggcac
    26641 agttgtggag cgtagatgcg ttgttggttt accatgctct ggtacataaa aaacgccagt
    26701 gccatggtaa cctgatatag aaacgttaat tccgcgacgt tcaaactcgg catagacgtg
    26761 ttctgctaaa gacctctttt gaccaaataa tgccctggcc aaccagccgt tattcatatt
    26821 tgcggatttt tctatttcat caggagagaa gtctttgttt tttattatgt tggctgagtt
    26881 acaggatgtt aatctgatat catctatttc atgaagatta tgttgaacaa tgcggtcgac
    26941 aatatctgac ggtgataaaa attcatctcc gcacttaaga agaggtagac cagcagaacc
    27001 atgcccagac agataaattt tgttgtattt tcctggtgtt aaatcatctg gcaggatggt
    27061 tttcatctct gtcgctgtaa tacttatagc tgcagcagca acaacatcgc tattacttga
    27121 ttgtagatgg cttttatttg ctccaggata tgtaaactcc attttccttt tatcaaaatc
    27181 ttgttttgta gcatcattca ggaataataa atcatacggt tttttccccg ttgtgactct
    27241 tgaaacattt tggccaagaa tattaagagt atagtcatta acggatttca ttcctaaaaa
    27301 aacaagtaat ccttctttct cttgcgcttt ttcgattgtt ctccctgcta aagttattgg
    27361 gatggattga gattgttttg tcggaactga tacgctggcg ctaataggta gtagggttct
    27421 tatgctaaac atacaacctc tttcttaatt ttcgaacctg tttaggattc tgtgtaaatt
    27481 caaaataaac ctgtctgaac attcctaaca aacatccacc ggacatgaca acaaaaaccg
    27541 gagccggact ccggtttttg tgaagctgtc ggctatttca ttccgccaat attttcccac
    27601 gtcccgtcag cacgcagaat ttgcagcggt cttaccacgc actgtatctg ctttttatcc
    27661 gcatccagta tcaccacctg cgtgattacc ctggcctgct ccgggataat gccattctca
    27721 tcggactcca gaatgtctgc cggtcccagt cgcagctgtg ctgtaggcga ctgcacgtgt
    27781 tcacggccat catgctttcc gcaaccacac agacactgca taagtttttt tagtatattc
    27841 atgtcattct cctgttctgc ctgtatcact gcccacttca tccagcccct taacatcctg
    27901 ccacggcccg tcaccaaacc tgacctgcaa atgccgaaac agcccctgaa cctgtgtggc
    27961 atctttgggg tcaagaaagg tcagtccggt gatgagtgcg ccatctgtat ccgggaacca
    28021 gccattgctg tttgtctcaa taatgctcgc cggccccaga cgaaaacgga tttgtgtctc
    28081 ccccgggtcg cccttcggtc cctgaggtcc ggttgccccc accgggccag ccgcacctgt
    28141 ttctcctttc ggtccctgtg ggcctgccgg gcctgccgca ccggtatctc cctttggacc
    28201 ctgtggacct gcatttcccg tcagaccggt ctctccccgc tctcccctgt cacctttcgg
    28261 cccctgcggg cctgccggac cagcatcacc tgccggtccc cgtt
//
LOCUS       DAZFJF010000059        28303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFJF010000059 DAZFJF010000000
VERSION     DAZFJF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.059"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..1140)
                     /gene="gldA"
                     /locus_tag="ACHKAU_002703"
     CDS             complement(37..1140)
                     /gene="gldA"
                     /locus_tag="ACHKAU_002703"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO5531731.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1151..1813)
                     /gene="fsa"
                     /locus_tag="ACHKAU_002704"
     CDS             complement(1151..1813)
                     /gene="fsa"
                     /locus_tag="ACHKAU_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5531732.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1825..4326)
                     /gene="ptsP"
                     /locus_tag="ACHKAU_002705"
                     /pseudo
     CDS             complement(1825..4326)
                     /gene="ptsP"
                     /locus_tag="ACHKAU_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4635..5714
                     /gene="frwC"
                     /locus_tag="ACHKAU_002706"
     CDS             4635..5714
                     /gene="frwC"
                     /locus_tag="ACHKAU_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO5531733.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5729..6049
                     /gene="frwB"
                     /locus_tag="ACHKAU_002707"
     CDS             5729..6049
                     /gene="frwB"
                     /locus_tag="ACHKAU_002707"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5531734.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6100..8397
                     /gene="pflD"
                     /locus_tag="ACHKAU_002708"
     CDS             6100..8397
                     /gene="pflD"
                     /locus_tag="ACHKAU_002708"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5531735.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8363..9241
                     /gene="pflC"
                     /locus_tag="ACHKAU_002709"
     CDS             8363..9241
                     /gene="pflC"
                     /locus_tag="ACHKAU_002709"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO5531736.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9243..9584
                     /gene="frwD"
                     /locus_tag="ACHKAU_002710"
     CDS             9243..9584
                     /gene="frwD"
                     /locus_tag="ACHKAU_002710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5531737.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9571..10422)
                     /gene="yijO"
                     /locus_tag="ACHKAU_002711"
     CDS             complement(9571..10422)
                     /gene="yijO"
                     /locus_tag="ACHKAU_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5531738.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10571..12304)
                     /gene="eptC"
                     /locus_tag="ACHKAU_002712"
     CDS             complement(10571..12304)
                     /gene="eptC"
                     /locus_tag="ACHKAU_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5531739.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12487..15138)
                     /gene="ppc"
                     /locus_tag="ACHKAU_002713"
     CDS             complement(12487..15138)
                     /gene="ppc"
                     /locus_tag="ACHKAU_002713"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO5531740.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15490..16641)
                     /gene="argE"
                     /locus_tag="ACHKAU_002714"
     CDS             complement(15490..16641)
                     /gene="argE"
                     /locus_tag="ACHKAU_002714"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO5531741.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16795..17799
                     /gene="argC"
                     /locus_tag="ACHKAU_002715"
     CDS             16795..17799
                     /gene="argC"
                     /locus_tag="ACHKAU_002715"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO5531742.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17807..18583
                     /gene="argB"
                     /locus_tag="ACHKAU_002716"
     CDS             17807..18583
                     /gene="argB"
                     /locus_tag="ACHKAU_002716"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO5531743.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18644..20017
                     /gene="argH"
                     /locus_tag="ACHKAU_002717"
     CDS             18644..20017
                     /gene="argH"
                     /locus_tag="ACHKAU_002717"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO5531744.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20284..21201
                     /gene="oxyR"
                     /locus_tag="ACHKAU_002718"
     CDS             20284..21201
                     /gene="oxyR"
                     /locus_tag="ACHKAU_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO5531745.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21184..22584)
                     /gene="sthA"
                     /locus_tag="ACHKAU_002719"
     CDS             complement(21184..22584)
                     /gene="sthA"
                     /locus_tag="ACHKAU_002719"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO5531746.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22915..23565
                     /gene="fabR"
                     /locus_tag="ACHKAU_002720"
     CDS             22915..23565
                     /gene="fabR"
                     /locus_tag="ACHKAU_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO5531747.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23565..23924
                     /gene="yijD"
                     /locus_tag="ACHKAU_002721"
     CDS             23565..23924
                     /gene="yijD"
                     /locus_tag="ACHKAU_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO5531748.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(23964..25064)
                     /gene="trmA"
                     /locus_tag="ACHKAU_002722"
     CDS             complement(23964..25064)
                     /gene="trmA"
                     /locus_tag="ACHKAU_002722"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO5531749.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25178..25368
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25433..27277
                     /gene="btuB"
                     /locus_tag="ACHKAU_002723"
     CDS             25433..27277
                     /gene="btuB"
                     /locus_tag="ACHKAU_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO5531750.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27222..28079
                     /gene="murI"
                     /locus_tag="ACHKAU_002724"
     CDS             27222..28079
                     /gene="murI"
                     /locus_tag="ACHKAU_002724"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO5531751.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6470 a         7120 c         7805 g         6908 t
ORIGIN      
        1 ttcaaactcc cggacaagcc gggagtttgg agtaggttat tcccactctt gcaggaaacg
       61 ctgaccgtac tggtcggcta ccagcagagc ggcgtaaacc tgatctggcg tcgcgccgcc
      121 aggcatgttg tggatggttt caccttctgc acatgccgct tctgccacaa ttcgcatttt
      181 cgccgggaca tcttctttaa tatccagttg agcgagagtt attggcaaac ctaccgcatg
      241 gctaagtgca gctacggttt cgatttcctc aaccggcgcg ttttccagta ccagctgcgt
      301 cagcgtaccg aatgccactt tttcaccgtg ataatagtga tgcgcgtccg ggatagcggt
      361 caggccgtta tgcaccgcat gagccgccgc cagtccgccg ctttcaaaac caacgccgct
      421 taaataggtg ttcgcttcaa tcacgcgttc cagcgcaggg gttactacat gctgttcggc
      481 ggcaagcatc gctttttcgc cttcttccag cagagtgttg tagcacagtt ctgccagtgc
      541 cagcgccgcc tgggtgcact tgccgcccgc catagtggta gcgccgctac gggaacaggc
      601 gcgtgcttca aaccaggttg ccagcgcatc gccgataccc gccgctaaca gacgtgcagg
      661 agcgcccgcg acgattttgg tgtcgacaat gaccatattc gggttatttg gcaacagtag
      721 atagcggtca aactcaccct catcggtgta gataacagac aatgcgctgc acggtgcatc
      781 ggtagaggcg atagtcggtg cgatcgctac cggaacaccc atgaaatgtg ccagtgcttt
      841 ggcagtatcg agggtttttc cgccaccgat accgagaatt gcgccacact gcgcagtctc
      901 cgcgatgcca cgcagacgat cgatctcatt ttgcgaacat tcaccgccaa acggcgcaat
      961 ttctactacc agtccagcat ctttaaagct tttctcgaca gtggattgag caaaacctaa
     1021 aacaaatttg tcacccacca ctaaccagcg ttctgccagc ggcttcaggt attcgcccag
     1081 acgattaatc acatcagcgc cctggatgta tttacccggt gattgaataa tgcggtccat
     1141 aattgctcct ttagagatga gtagtgccaa atgcggcatt ccagtcgtgt tcgaacttct
     1201 ctatagctga ctctaccgca ggggtgttga gcatttgttg cgctacatct aaaggcaggg
     1261 tgatggactc acatcctgcc agcaaacaat ccagtgcctg acgcggcgtt ttaaagctgg
     1321 ctgcaagcac catgctttct ggcgcgtgca tttctaacag cgcttgcagc tcctgaaccg
     1381 tacgaatgcc gtccccgccc tgggcatcta cgcggttaac atacggagca acgtattttg
     1441 cccctgccag cgcggccagt aacccttgtg cagcactgta cactgcggtg ccaagcgtag
     1501 taataccctc ttttttcagc atcttaatgg cggccagacc ttcggaagtc accgggattt
     1561 tcaccacaat gcccggaatg gcgtcgcgca ggcgcttcgc ttcttccacc ataccctgcg
     1621 cgtcacgact catagtctga gcaaacagaa tgccctcatc accaatcgct ttttgcagac
     1681 gcggcagaac ttcccatatg gactccttgc tggcagcgat aatgctcggg ttagttgtca
     1741 ccccggcaat ggggaatatg cgtgccagac gttcgacttc tgcgacgtta gcggtgtcca
     1801 gatacagttc catgatgttt ccttttacag ttccagttca tgttgcagca ggctggcgat
     1861 agcgtcggca gaggcggcgt taaccagcgc gttacggaat tcttcgtgca tgatgcggcg
     1921 agcgaggcgc gagaaaatgc gcatgtgctg atcgcccgca gcgtgtttgt tcagggttaa
     1981 cataatgatg aattgcgctt catcatcgcc ccagcgcacc ggcgcttgca gacgcgctac
     2041 actgatggtg gattgctcaa tgtgttctga tttgctgtgg ggaatggcaa aactaaagcc
     2101 cagaccggta gagaaaacgg cctcgcgcgc ccacaagtcg gcttccagtt tacgcggata
     2161 gcggcagcgg cccgccagca gcaggttatc ggtcatgcct ttgagcactt cttctttgct
     2221 gcgtcagtcg ctttccagcg tgatgcactc ggcggtgacc agcggtgcgt cctgttgggt
     2281 catgcggaat tgcgccagca ggtgttccac ttccagcgaa gtacggcagg ccattgcctg
     2341 gttgagcaac tggcggcatt cgcggctatc aagttgcgcc attcgcgctt tcgccgccgg
     2401 aattgacggt gcgctcatgc tcagctcatc cagccctaaa ccgaccagca acggcagcac
     2461 ggaacctttc gctcccagct caccgcacag accaatccat ttgccctggc gatgcaccgc
     2521 ttgcacggcg taatcgagcg cccgcaagaa tgccggattc aggctgttgt agtgacgagt
     2581 aaccttagca ttatcgcgat ccaccgccag cagatattgc gtcaggtcat tactaccaat
     2641 actaaagaaa tcaatctctt cgcagcattg atcgatgatg aacatcaccg acggcacttc
     2701 cagcataatg ccgagctgga tcttctcatc aaacggaatg tgttcgttac gcagttgctg
     2761 tttggcttcc gccagttttt ctttcaccca taagatctct tccattgaag agatcatcgg
     2821 gatcatgatt ttcaggctgc cgtgagcgga ggcgcgcagg atcgaccgta gttgtgtggt
     2881 gaacagcgag gcgtattctt cataaatacg cacagcgcga taaccgagga acgggttagc
     2941 ttctgccgga atgttcagat aatcaacggg tttgtcgccg ccaatgtcca tagtgcgcac
     3001 aataatgctg cgtccgttgg cagactccag cgcctgacaa aaaatgttgt acagctcgct
     3061 ttcgcccggt gcactggtgc gatccatata gagcatttca gtgcggaaca aaccaacgcc
     3121 ttccgcacca ttgccgaatg cggcctgcgc ttccacggag tgagcgatgt tagcggcaat
     3181 ttcaatgcgg ataccgtcag cggtacgggc ttgttgggtc agccagacac gctgttgctc
     3241 acgcagggca tcctgtacgc gggcttcttg ctgataataa cgggctacgg cttcccctgg
     3301 ctcaaccaca atcgctccgg cgttgccgtc gatataaatc gtttgatgtt gccacggggt
     3361 aagggcatca atatccacac caaccagcgt cggaatgttg aacgaacggg caaggatcac
     3421 cgtatgtgag gtggtgccgc cgcttttgag caacaatcct ttgaggtgat ttttatccag
     3481 ttcgaggaac tggctggggg tcagttcatc agccatacaa atggcgggct gcgtcagttt
     3541 gcccggtgcc gggaagcgtt gctcaccgta gatttgctgg agtaactgga agcagacgtc
     3601 gcgtacgtcc agggcacgtt cttgcaggta gctgctgctg gaacgggaaa actcttcaca
     3661 aaagtgattc gcactggcga caatcgcttc ggcgcagctt aatccggcgc tgacgcctgc
     3721 cagtaaatgt tcgtgcaggg aagtatcgcc agccagggat cggtgcgctt ccagaatcgc
     3781 gctggtggca ccgtcgctat ccagcagacg aaactcaatg tttttcagta ccagcgtcag
     3841 gccgttttcc agtgcggatt gctcggcgtc aacgtctttg gctgcgggaa gattactcag
     3901 cgcattgaga tctaaagaag agatcggcgt cagaatgccg cctgcgctac cgctgcacac
     3961 ggtgcgggcg cggataattt gcggattcag attggtcagt gaaaccggca gtggttccag
     4021 ttcgtcagat ttaacttccg ccagcggtgc gtcgcagtgg gggaattcat cgcgcagcca
     4081 ttggcttaaa cgctggtgcg cttcctgttc gtcggccccg gaaatcagta accggcagtt
     4141 atcgcccgcc agcgtgtcgg tgccaatcag cgcaagggcg cttttggcgt tgcccttgcg
     4201 gtcagtgcgc aggttatgcc actcaatttg tgatgaaaaa gtattacaca gcgtttcaac
     4261 gtggcttgcc ggacgtgcat gtacgccgtt aggtagctca caaataaatt ccacaatcag
     4321 ggccattgcc tctctcccgg tacggttttc tgtttacagg ataagagcgc acggcaacgg
     4381 cctgccatgt gacaaatctg ccaaaagctg gacaaatgta atgtaaccgt caatttgcga
     4441 cgcgtctcac aagacgccgt tttgcggcat gcttccggat tatcgcaagt tatgaggcgg
     4501 atcgcatttt tgtactgata ttacaaaagt ccagtaaatg gcctttttat ccactgtttg
     4561 ctccgcctgc gattgcctat tgtttcctcc atcaaaaata tggacatggg ctttcgcaag
     4621 cccgggagca gcttatgaat gagttggtgc agatcctgaa aaatacccgt cagcatttaa
     4681 tgacgggcgt ttcacacatg attcccttcg tggtatcggg cggtattttg ctggcggttt
     4741 ccgtcatgtt gtatggcaaa ggcgcagtgc cggatgccgt agccgatccg aatctgaaaa
     4801 aactgtttga tatcggagtt gcaggtttga cgctgatggt gcctttcctc gccgcttaca
     4861 tcggttactc cattgcagag cgttctgcgc tggccccgtg cgctatcggt gcctgggttg
     4921 gtaacagctt tggtgcgggc ttctttggtg cgctgatcgc cgggattatc ggcggcatcg
     4981 tggtgcatta cctgaagaaa attccggtgc ataaagttct gcgctcggtg atgcctatct
     5041 tcattattcc tatcgtcggc acactgatta ccgcaggcat catgatgtgg ggcttgggcg
     5101 agcctgtagg ggcgttgacc aacagcctga ctcagtggct tcaggggatg cagcagggca
     5161 gcattgttat gctggcggtg atcatgggtc tgatgctggc gttcgatatg ggcggtccgg
     5221 ttaacaaagt ggcctatgcc ttcatgctga tttgcgttgc tcagggtgtt tataccgtgg
     5281 tggccattgc tgccgtgggt atttgtatcc cgccgctggg gatggggctg gcgacgctga
     5341 ttggtcgtaa aaatttctcc gcagaagagc gcgaaaccgg caaagcggcg ctggtgatgg
     5401 ggtgcgttgg ggttactgaa ggggcgattc ctttcgccgc tgccgatccg ctgcgtgtca
     5461 ttccttccat catggtcggt tctgtttgtg gtgcggtaac tgcggcgctg gtcggtgcgc
     5521 agtgctatgc aggctggggt ggtctgattg tgctgccggt ggttgaaggc aagctgggtt
     5581 atatcgcagc agtggctgtc ggagcagtgg tgacggctgt ttgcgtgaac gtgctgaaaa
     5641 gtctggcgcg taaaaatggg tcttcgactg atgagaagga agacgacctg gatttggatt
     5701 ttgaaattaa ttaattgagg aacgagccat gacgaaaatt attgcagtaa ccgcgtgtcc
     5761 atctggtgtt gcccatacct atatggcggc agaggcgctg gaaagcgccg cgaaagcgaa
     5821 aggctgggaa gtgaaagtag aaactcaggg gtcaattggt ctggaaaacg aactgactgc
     5881 ggaagacgtg gcgagcgccg atatggtgat tctgaccaaa gatatcggca tcaagtttga
     5941 agagcgtttt gctggcaaga ccatcgtgcg cgtcaacatc agcgatgcgg taaaacgtgc
     6001 tgacgccatt atgagcaaaa ttgaagctca cctggcgcaa accgcttaag ttcttctccc
     6061 gctcgcaagg gcgggttcgc tttcccacag gagttcctca tgacgaatcg tatctctcgc
     6121 ctcaaaactg cactgttttc caatacccgt gaaatctcgc tggagcgggc gctgctttat
     6181 accgccagcc atcggcaaac cgaaggcgaa ccggtgatat tgcgccgggc gaaagcaaca
     6241 gcgtatatcc ttgaacatgt tgaaatttcg attcgtgatg aagaactgat tgccggtaac
     6301 cgcaccgtaa aaccgcgcgc cgggattatg tcgccggaaa tggaccctta ctggctgctg
     6361 aaagagctgg atcaattccc gacgcgtccg caggaccgct ttgctatcag cgaagaagat
     6421 aaacgtatct accgcgaaga gttgttcccg tactgggaaa aacgttcgat gaaagatttc
     6481 atcaacgggc agatgacaga tgaagtaaaa gccgcgacca gcacgcagat tttcagcatt
     6541 aaccagacgg ataaaggcca ggggcacatt attattgatt acccacgcct gctgaatcac
     6601 gggctggggg agctggtggc gcagatgcag caacattgtc agcaacagcc ggagaatcac
     6661 ttttatcagg ccgcgctgtt actgctggaa gcctcgcaga aacacattct gcgttacgct
     6721 gaactggcgg aaacaatggc ggcaaactgc acagatgccc agcgtcgcga agaactgctg
     6781 actattgcgg agatctcccg ccataacgcc gaacataagc cgcagacgtt ctggcaggcg
     6841 tgccagttat tctggtacat gaacatcatt ctgcaatacg aatccaacgc cagttcgctg
     6901 tctttagggc gcttcgacca gtatatgttg ccgttctatc aggcttcttt aacccagggc
     6961 gaagatccgg cgttcctgaa agaactgctc gaatctttat gggtgaaatg caacgacatc
     7021 gtgctgttgc gctccaccag cagcgcgcgt tatttcgcag gtttcccgac cggctatacc
     7081 gcactgctcg gcgggttaac cgagaacgga cgtagcgcgg tgaacgtgct ttcgttcctt
     7141 tgccttgacg cctatcaaag cgtgcaatta ccgcaaccga acctcggcgt gcgcactaac
     7201 gccttgatcg acacgccgtt cctgatgaaa accgccgaaa ccattcgcct cggcaccggt
     7261 attccgcaaa tctttaacga tgaagtggtg gtaccagcgt tcctcaaccg tggcgtttcg
     7321 ctggaagatg cgcgcgacta ttccgtagtg ggctgtgtgg aattatctat tcccggcaga
     7381 acctacggct tgcatgacat cgcgatgttt aatctgctga aagtgatgga aatctgcctg
     7441 catgaaaatg aaggcaatgc tgcgctgact tatgaaggtt tactggagca gatccgcgcc
     7501 aagatcagcc actacatcac cctgatggtt gagggcagca atatttgtga tatcggccat
     7561 cgcgactggg cacctgtacc gctgctctcg tcttttatca gcgattgtct ggaaaaaggt
     7621 cgcgatatta ccgatggcgg cgcgcgttat aacttctccg gcgtacaggg gatcggtatc
     7681 gccaacctga gcgattctct ccatgcgttg aaagggatgg tttttgagca acagcgttta
     7741 agttttgacg aattgctgtc ggtattaaaa gccaactttg caacgccaga aggcgaaaaa
     7801 gtccgtgctc gcttaattaa ccgcttcgag aaatacggta acgatatcga cgaggtggat
     7861 aacatcagcg ccgaactgtt acgccactac tgcaaagaag tggaaaaata ccagaacccg
     7921 cgcggtggtt acttcacgcc gggatcgtat accgtttctg ctcacgtccc gttgggatcg
     7981 gtggttggcg cgacgccaga cggtcgtttt gccggggaac aactggcaga cggcggcttg
     8041 tcacctatgc tgggccagga cgcacaaggg ccaacagcgg tactgaagtc agtcagtaag
     8101 ctcgataata cgctgctgtc taacggtacg ttgctgaacg tgaaattcac tccggcgacc
     8161 ctggaaggtg aagcgggatt acgcaaactg gccgacttct tacgggcgtt tacccagctt
     8221 aagttgcagc atattcagtt taacgtggtg aacgccgaca cgttgcggga agcgcaacag
     8281 cgcccacaag attatgccgg gctggtggtg cgcgttgccg gatacagcgc cttctttgtc
     8341 gaactgtcga aggagatcca ggatgacatc atccgccgga cagcgcatca gctgtaacgt
     8401 tgtggaaacg cgccgcaatg atgtggcgcg cattttcaac attcagcgtt attcactgaa
     8461 tgacggcgag ggcattcgta cggtggtctt ttttaaaggc tgtccgcatc tttgcccatg
     8521 gtgtgctaat ccggagtcga tctccggcaa aatccagacg gtacgcagag aggcgaaatg
     8581 tctgcactgt gcgaaatgtt tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg
     8641 gattggtcgc gatatcagcc ttgacgcgct ggaacgggaa gtgatgaaag atgacatttt
     8701 ctttcgcacg tccggcggcg gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga
     8761 gtttgctacc cgttttttac agcggctgcg gttgtggggc gtgtcatgcg ccattgaaac
     8821 tgccggagac gtgccagcca gcaagctgtt accactggcg aaattgtgcg atgaagtgct
     8881 gttcgattta aaaattatgg acgcgactca ggcgcgggat gtggtgaaga tgaacctgcc
     8941 acgcgtgctg gagaatctgc gtttgctggt gagtgagggt gtcaacgtga tcccgcgttt
     9001 accgttgatc cctggtttca cgctcagccg ggagaatatg cagcaggcgc tggatgtact
     9061 gatcccgctg aaaatcaggc agatccatct gttaccgttt catcagtacg gcgaaccgaa
     9121 ataccgcctg ctggggaaaa catggtcgat gaaagaggtg cctgcgccgt cgtcagccga
     9181 tgtgacaacg atgcgcgaaa tggcagaacg ggccggattt caggttaccg tgggaggtta
     9241 aaatggcata cctggtggca gtaaccgcct gcgtaagcgg cgtggcgcat acttatatgg
     9301 cggcggaacg gctggaaaag ttgtgccagt tagagaagtg gggcgttagc attgaaactc
     9361 agggcgcgct gggaacggag aatcgtttag cggacgagga tatccgtcgg gcggatgttg
     9421 ctctgttgat tacggatatc gagcttgccg gtgccgagcg atttgaacat tgccgctatg
     9481 tgcaatgcag catctacgca ttcctgcgtg agccgcagcg ggtaatgagc gcggtgcgca
     9541 aagtgctttc tgcgccgcag caaacccatc ttattctgga gtagtcggtt tttctgtcag
     9601 ttggctgtgg tactgccgac gatattccga cggcgagcgt tcggtgtttt tacgaaacag
     9661 acggcagaag tagttgctgt cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag
     9721 gtcatagcct ttcaataacg tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt
     9781 aaaaccaatg gcccccgttt tttgaaacag gtgcgagaga tagtttggtg aaatatagaa
     9841 tgcctgtgcg acagattcgc gggtaagcgc ggaggcatag cgttcgtcga tataatcgcg
     9901 aatagcttca aatagtgcct ggctgcgtga ggcggtctgg atttggctgc cgagcaaatc
     9961 gcggcagtgg ctaagcaggc tggcgacgat aagccttgcc gtttgctgct cctgcggctg
    10021 catttgcatt tcattgagcg tttgtagcag aaaagaacct atgcgtgggc cgcgccgggc
    10081 gacgtgttgc ttcgccagat tttgatattg tttgccatcc cattgcacga cgctgaagcc
    10141 gagttgctgt ttgccaaaca gcacgctaaa ggtagtagcg ggggcttgcc attgtggaaa
    10201 attccagcca ccagcaggaa cgtacagcac atcgccggga actaacgttg ctccagcgcc
    10261 ggtatcaaca aactcacctt ccagcacaat ttccagccgt ggaaaatcga cctgatacgc
    10321 gagatcgggc accgggccgt tactgctggc aaagtaaatc tggcgcaggg acagcgggcc
    10381 gttgatcagg cgggagagca gatagctgac gtcgtgatac atgttattcc cttaggtatg
    10441 tagacgggaa aacagtaact ggcattatta aaaaaggcca cctgaaaagt gtggcctgag
    10501 gggagttcga tgtaaacatt gcggcagtat cgcatccggc aaatagcgcc agacgcgata
    10561 caaaggtcat ttactgatta cccacctgat cgccatacgg cagcgtgtcg taatcgatca
    10621 gcgcgttttt cttgtacggg ttaccaatcc agcgggtagt ttctttgaac tgcggattca
    10681 ccactgaacg ggttggatcg taaccgtcgt aagataagcc tgctaaatct gaccaggtgt
    10741 ggatcagttc cgccaggctg tatttacgat caacatcctg cgagaaatca cgcggatgag
    10801 tcgcttgcca tttttccgac gtccacagca ggaacggaat ggtgtacatg tggcgcgtcg
    10861 ggttgtcttc gttacgcccc tgagttttgt gcggcggcgt gtcgtaaacc tcttcaccgt
    10921 ggtcagagaa ataaaccagg aaaccgttcg ggtttgctgc tttaaagtct ttaatcaggc
    10981 tggcaaccac atgatcgtta tacaggttag cgttgtcata atcgttatat gactccagct
    11041 cttccgcgtt taatccaggc ggaacatgat cggtattgcc atcaaacttg ccctgatttt
    11101 ccgggtagcg gtatttgtat ttgatatgcg tacccagcag atgaacgatg atcagtttct
    11161 tcggcgccgg gtccttcagc acttcctgga atggtttcag cacgttggtg tcgtattcac
    11221 gcgcactctg cgtacgttgc tggttcatat agaactgttt gtcggtctgc ttcgaaaata
    11281 ccgtcagcat ggtattgcgg gcggtcatcg tctgctggtt ggtgatccag aaggttttat
    11341 aacctgcctg tttcatcatg ttcatcagcg acggttgtgt caggtacagg tccgggttct
    11401 tttcattagc aaaggtcagc gcctgttgca ggatttcaat ggtgtacgga cgagacgtaa
    11461 ccacgttatt aaacacggtc agattcggat cggttttatg cagcgcatcc agctccggcg
    11521 tggtttcacg cggataaccg tacagactca tgcgcccgcg ttgggtcgat tcgccaatca
    11581 ccagcactaa ggtacgcggt tcgttacccg attcatcttt gaaattagcc agaggcggca
    11641 aggcattatt ttcattcagt aactttgtta gcgagtttag ttgctgacga tactgataat
    11701 agccggtcag gaattgccac ggtgcggcag gctccatacg cgaggccagg ttatccaacg
    11761 ttttctcaaa cggcttgttt ttgataaacg tattcatggc gatcggatgc agaatcaagc
    11821 cataaagcag ggcaaaagag acaacataac gccacggctt tggaatatag accgggtgca
    11881 ggcgtgtcca cagcagaact gccaccgccg tataggccag cgcgataagc acaattttca
    11941 ggctgaaata ctggcttaaa tactcgctgg cttcgttggt gttggtttcg aacatcacaa
    12001 acagaacgct ctgcgagaac tcctgcccgt agatgacgta gtagcatagc gccgccagag
    12061 aggccgccca tagcaccacg ccgattactg cggcaataat tttaatccgc ttcggaaaga
    12121 ggaataccgg gatcaaccac agcgagctga ataacagcga gtcgcgaatg ccgttggtgc
    12181 cactataacc actgatgtaa ataatggcct gtagcagagt agagaaaaac caaaagtaga
    12241 gcagtgccca acccagggct ttccagctaa aaaggggttt agcctggact tctgtggaat
    12301 gcatagtaag aacctgtctt gaaaaaaata tcgccgaatg taacgacaat tccttaagga
    12361 tatctgaagg tatattcaga atttgaataa aatgcagaca gaaatatatt gaaaacgagg
    12421 gtgttagaac agaagtattt cagaaaaccc tcgcgcaaaa gcacgagggt ttgcagaaga
    12481 ggaagattag ccggtattac gcatacctgc cgcaatcccg gcaatagtga ccattaacgc
    12541 ttgttcgacg cgaggatccg gttcctggcc ttctttttct gcctggcggg agcggtgcag
    12601 taactcggcc tgcaatacgt tcagcgggtc ggtgtaaata ttccgtagct gaatagactc
    12661 tgcaatccac ggcagatcgg ccattagatg ggaatcgttg gcaatcgcca gcaccacttt
    12721 gatgtcttct tcttgcaggt tgcgtaactc tttacctaac ggccacagtg ctttgtctac
    12781 caggcgttgg tcatagtatt ccgccagcca caggtctgct ttggcgaaga ccatctccag
    12841 catgccgaga cgcgtcgaga agaatggcca atcgcggcac atggcttcca gctcgctctg
    12901 tttgccatct tcgaccactt tttgcagcgc cgtacctgca cccagccagg cggggagcat
    12961 caggcggttt tgcgtccagg caaaaatcca cggtatggcg cgcagcgact cgacgccgcc
    13021 ggttgggcga cgtttcgccg gacgtgaacc caacggcagt ttgcccaatt cttgttccgg
    13081 cgtagcggag cggaagtaag gcacaaaatc tttgttttca cgtacgtagc cacggtagag
    13141 atcgcaggag atgactgaca gttcatccat aatgcgacgc cagctctctt tcggctcagg
    13201 tggtggcagc aggttggctt ccagaatagc cccggtgtac agcgacaggc tgctgacggt
    13261 gatttctggc agaccatatt taaagcggat catctcgccc tgttcagtca cgcgcaggcc
    13321 acctttcagg cttcctggcg gttgtgacag cagcgccgca tgagcaggtg cgccgccgcg
    13381 accaatggaa ccgccgcgac cgtggaacaa cgtcagctca atacccgctt tttcgcaggt
    13441 tttgattaat gcatcctgtg cctgatattg cgcccaggaa gctgccatca ctcccgcatc
    13501 ttttgctgag tcggaatagc caatcatcac catctgtttg ccctgaatca ggccacgata
    13561 ccagtcaata ttgagcagct gggtcatgac atcgttggcg ttgttcagat catcgagggt
    13621 ttcaaacagc ggagcaaccg gcatcgcaaa cccgataccc gcttctttca gcagcaggtg
    13681 gacagccagt acgtcggacg gcgttttcgc catcgagatc acgtaggcgg caatggagcc
    13741 ttgcggtgct tcggcaatca cctggcaggt atcgagcact tcgcgcgttt cggcgcttgg
    13801 ttgccagttg cgcggcagaa gcggacgttt ggagttcagt tcgcggatca ggaacgcctg
    13861 tttgtcggcc tctgaccagc tttcgtagtc gccgataccg aggtagcggg tcagctcgcc
    13921 cagcgcttcg gtatgacgcg tgctctcctg acggatatca atacggacca gcggtacgcc
    13981 gaaacatttc acgcggcgca gggtgtcgag cagatcgccg ttggcgataa tacccatgcc
    14041 acacgcctga agtgactggt agcaagcgta gagcggttcc cacagttctt cgttttgtgt
    14101 cagcaggcct tctggttttg gcagttcttc gcctttcagg cgcgcttcca gccatgcctg
    14161 tgtcgccatc aggcgagaac gcaggttttt catcagatag cgatacggtt ctgcggcacc
    14221 ttcttcgcca accagcgcca gcagttcagg ggtcgcttca accatcgaca gttcagaaac
    14281 cagcacctga atatctttca ggaacaaatc ggtggctttc cagcggctga gtagcaggac
    14341 gtggcgggtg atatcggcag tgacgttcgg gttgccgtcg cggtcgccgc ccatccacga
    14401 agtaaaacgg accggaacaa attcgacggg cagtttgtag ccgaggttct cttccagttg
    14461 ttcgttcagt tcgcgcaggt aatttggtac gccttgccac aggctgtttt ccactacggc
    14521 aaagccccat ttggcttcat ctaccgggct tggacgcagc ttacggattt catcggtatg
    14581 ccatgactgg gcgattaact ggcgcaggcg gcgcatcagc tggttgtgtt cgtagtcagc
    14641 gatatctttg ttatcgagct gttttaaaca ggcgttcact tccaccattt tgtggatcag
    14701 tgtacgacgg gtaatttcgg ttgggtgagc cgtgaggacc agttccagcg acagcgattc
    14761 cactgctttt ttgatggtgt cttcgctcag ttccggctgg tttttcagtt tacgcagggt
    14821 gcgggcgatc acttccgggt tgctggcagc ttcgcctttc ggcgaaatgc tgtggtattg
    14881 ctcggcggtg ttggccaggt tcaggaactg actaaacgca cgcgcaacgg gcagcagctc
    14941 gtcgttcgac aaattttgta aggtggtgag caactcctgg cggttagcat cattgccagc
    15001 gcgtgaagat ttcgacaact tacggatagt ttctacgcgt tcaagaatgt gttctcccaa
    15061 cgcatccttg atggtttctc ccagcacttt gccgagcata ctgacattac tacgcaatgc
    15121 ggaatattgt tcgttcatat taccccagac accccatctt atcgtttgat agccctgtat
    15181 ccttcacgtc gcattggcgc gaatatgctc gggctttgct tttcgtcgtc ttttataaag
    15241 ccacgtaaaa gcggtgacgt caaatgctgc gaaatcgctt cagcaaacga ataaatagca
    15301 ggaatttacg ccattaaatt cacgacgctt taaataagcg taacttatgg aaatgttaaa
    15361 aaatcgccat acataacacc aaaggtgtag gtcggataag atgcgcaagc atcgcatccg
    15421 acattattgc ggcacaggag tttggcaacg gtgccggatg cggcgcgagc gccttatccg
    15481 gcctacgttt taatgccagc aaaaatggtg aattacctgg gttatcagtt cgcgggtggg
    15541 cttgataaac cgtgtttcca gatattcatc aggttgatga gcctgattaa ttgagccagg
    15601 ccccaacacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac agtagttcac
    15661 cacttcggtc tttgctccga gcaatttctc aactacttca accagttgat gattcggtgg
    15721 gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac ccggccagcg
    15781 ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga gtgtcatgcc
    15841 aggcagcgga cgaatatcca tatgcaactc acagcaagcg caaatacggt tagaagcgtc
    15901 gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaacg cttcgtagtg
    15961 ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt cgtgcattag
    16021 ttcgatagcg ttaactccgc gtgctggatc gctggagtgc cccgactggc cctgaatacg
    16081 gatggcgtta gagatatgac ctttatgtgc gcgtaccggt tgtagtgacg tcggttcgcc
    16141 aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg ctccggccat
    16201 actggtttct tcatcagcag tcgccagaat gtagagcggt tttttcagtt tcgtgacgtc
    16261 gacatcgcgt agcgcatcaa ggataaacgc aaaaaagcct ttcatgtcgg cggtgcctaa
    16321 gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc agcgaccgtc
    16381 atcaaatggc accgtatcgg tatgccccgc cagcaacaag ccgccagccc cctgtccgat
    16441 acttgccagc atattgaatt tgttgcgagt tcctggcaca ggctgcactt ccacattgaa
    16501 gcctaaatct ttaaaccagt ccgccagcag agtgattaaa tctgcattgc tttgatcgag
    16561 tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa tctcgataaa
    16621 tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat caatattcat
    16681 gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac cagccgtaag
    16741 gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc cccgatgttg
    16801 aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac ctatgtaaat
    16861 cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa tgatgcggga
    16921 aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgatctgcc gttgcagccg
    16981 atgtcggata tcagcgagtt tagcccaggg gtggacgtag tgtttctcgc caccgcccat
    17041 gaagttagcc acgatttagc gccgcagttt cttgaagcgg gctgcgtggt gttcgacctt
    17101 tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg ctttacccat
    17161 caatacccgg aactgttgga acaggcagcc tacggtctgg cggagtggtg cggtaataaa
    17221 ttaaaagaag cgaatttgat tgcggtgccg ggctgttatc cgacggcggc acagctggcg
    17281 ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt gatcaacgcc
    17341 accagcggcg tgagcggtgc aggacgtaag gcagcgattt caaatagttt ttgtgaagtt
    17401 agcctgcaac cgtatggcgt ctttacccat cgccatcaac cagagatcgc cacacacctc
    17461 ggtgctgacg ttatcttcac cccgcatctg ggcaatttcc cgcgcggcat tcttgaaacc
    17521 attacctgcc gcctgaaacc gggtgtgacc caggcgcaag tggcgcaagc gttacagcag
    17581 gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc gctgaaaaat
    17641 gtcgttgggc tgccatgttg cgatatcggg tttgccgttc agggcgagca tctgattatt
    17701 gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca gtgcgccaat
    17761 attcgtttcg gctatgcgga aacgcagtct cttatttaag ggtgcaatga tgaatccatt
    17821 aattatcaaa ctgggcggcg tactgctgga tagtgaagag gcgctggaac gtctgtttag
    17881 cgcactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtgc acggcggcgg
    17941 ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga aaaatggcct
    18001 gcgggtgacg cctgcggatc agatagacat tatcaccgga gcactggcag gaacggcaaa
    18061 taaaaccctg ctggcatggg cgaagaaaca tcagattgcg gccgtaggtt tgtttctcgg
    18121 tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggtcatg ttggactggc
    18181 gcagccaggt tcgcctaagc ttatcaacac cttgctggag aacggctatc tgccggtggt
    18241 cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg accaggcggc
    18301 gacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctctgatg tcagcggcat
    18361 tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag aacaactgat
    18421 tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc tggatgctgc
    18481 gcgcacgttg ggccgtccgg tagatatcgc ctcctggcgt catgcggaac agcttccggc
    18541 actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt tggccggagg
    18601 cgcagctttc cggcattgaa tttcaaaata aggaaacaga gttatggcac tttggggcgg
    18661 gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac tgcgctttga
    18721 ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag ccctggtaac
    18781 ggtcggcgtg ttaaccgcag aagagcaggc gcaactggaa gaggcgctga acgtgttgct
    18841 ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag atatccatag
    18901 ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc tgcataccgg
    18961 gcgtagccgt aatgatcagg tagcgacaga cctgaaactg tggtgcaaag ataccgttgg
    19021 cgagttgctg acggctaacc ggcagctgca atcggcgctg gtggaaaccg cacaaaacaa
    19081 tcaggacgcg gtgatgccag gttacactca cctgcaacgc gcccagccgg tgacgttcgc
    19141 gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt tgcaggatgc
    19201 gcttaagcgt ctggatgtca gcccattagg ctgtggcgcg ctggcgggaa cggcctatga
    19261 aatcgaccgt gaacagttag caggctggct gggctttgct tcggcgaccc gtaacagtct
    19321 cgacagcgtt tctgaccgtg accatgtgtt ggaactgctt tctgctgccg ctatcggcat
    19381 ggtgcatctg tcgcgttttg ctgaagatct gattttcttt aacaccggcg aagcggggtt
    19441 tgtggagctt tctgaccgcg tgacttccgg ttcatcatta atgccgcaga agaaaaaccc
    19501 ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggggcgt taaccggcat
    19561 gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg aagacaaaga
    19621 aggtctgttc gacgcgctcg atacctggct ggactgcctg catatggcgg cgctggtgct
    19681 ggacggcatt caggtgaaac gtccacgttg ccaggaagcg gctcagcagg gttacgccaa
    19741 cgccaccgaa ctggcggatt atctggtggc gaaaggcgta ccgttccgcg aggcgcacca
    19801 tattgttggt gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg aagatctgcc
    19861 gctcgacgag ttgcagaaat tcagcccggt gattgacgaa gatgtctatc cgattctgtc
    19921 gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc agcaggtggc
    19981 gcaggcaatt gcttttgcgc aggcgcggtt ggggtaagaa catttatatg tataaatttg
    20041 agcctggctt atcgccgggc ttttttatgg caaaaaaaag cggatcctgg agatccgcaa
    20101 aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc agtgacttca
    20161 agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa tgcttgatag
    20221 ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcaa tggaggatgg
    20281 ataatgaata ttcgtgatct tgagtacctg gtggcattgg ccgaacaccg ccattttcgg
    20341 cgtgcggcag attcctgcca cgttagccag ccgacgctta gcgggcaaat tcgtaagctg
    20401 gaagatgagt tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt gttcacccag
    20461 gcgggaatgc tgctggtaga tcaggcgcgt accgtactgc gtgaggtgaa agtccttaaa
    20521 gagatggcaa gccagcaggg cgagacgatg tccgggccgc tgcacattgg tttgattccc
    20581 acagttggac cgtacctgct accgcatatt atccctatgc tgcaccagac ctttccaaag
    20641 ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact ggacagcggc
    20701 aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat tgaagtgccg
    20761 ttgtttgatg agccaatgtt gctggctatc tatgaagatc acccgtgggc gaaccgcgaa
    20821 tgcgtaccga tggccgatct ggcaggggaa aaactgctga tgctggaaga tggtcactgt
    20881 ttgcgcgatc aggcaatggg tttctgtttt gaagccgggg cggatgaaga tacacacttc
    20941 cgcgcgacca gcctggagac gctgcgcaac atggtggcgg caggtagcgg gatcacttta
    21001 ctgcctgcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta tctgccgtgc
    21061 attaagccgg aaccacgccg cactattggc ctggtttatc gtcctggctc accgctgcgc
    21121 agccgctatg agcagctggc agaggccatc cgcgcacgaa tggatggcca tttcgataaa
    21181 gttttaaaac aggcggttta aaccgtttaa cgcagctacc cgataggctt ccgccatcgt
    21241 cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac ctttctgttc
    21301 cataatcgcc tgaccgatat gaataatttc ggcagcgcgc tcgccaaagc agtgaatacc
    21361 cagaatctct tttgtttccc gatggaacaa aattttcagc gtgcccacgt tcatgccgac
    21421 gatttgtgcg cgtgccagat gtttaaactg ggcgcggccc acttcatatg gcactttcat
    21481 tgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg tgtaaatgcc
    21541 ggtagggata tcttcaatca gatgtgcggt ggcttcgcct tttaccagcg cctgcgcggc
    21601 aatgcgcccc tggtcatagg ccgctgatgc caggctcgga taaccaatca cgtcgcccac
    21661 cgcgtaaacg tgcggctgtg cggtctgata catgctgttg accttcagct gtccgcggct
    21721 gtcagtttcc agcccaatgt tctgtaacgc cagcgaatcg gtattaccgg tgcgaccgtt
    21781 ggcatagagc aggcagtcag ctttcagttt tttacccgac ttcaaatgca tgatcacacc
    21841 atcgtcacag ccttcgatct tctcgtactc ttcgttgtga cgaatcacta cgccactgtt
    21901 ccagaagtga taggagagag aatctgacat ctcttgatcg agaaatgcca gcaggcgatc
    21961 gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg catattcaca
    22021 gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca tgctaagaat
    22081 tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat gatatggacg
    22141 agagccgcag gcaataacaa atttttcagc ggttagtgtt tcaacgctgc cgtccgggca
    22201 atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttt ccctgcaata tttcacagtg
    22261 attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt tatcggcatg
    22321 gttaaggata tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt aaagtgggtt
    22381 ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg ggatggtgcc
    22441 ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga cgcgcgcacc
    22501 ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta ttactatggc
    22561 atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat aaaagcaaca
    22621 gaatggtaac attttatcgc gggtaagcca attgatcccc gtcatttatc tggctatatc
    22681 ctgagcggcc tttgctttgt ctgtttctta cttttgccct gacgttttat tggattttta
    22741 tcgacgatac tctccgttta agcggcaggt ttccgctgta cgtaaaagaa ccggccaaag
    22801 aattgcagta aatatgtttt attgcgttac cgttcattca caatactgga gcaatccagt
    22861 atgttcattc tctggtatag tgccagcagt acttttggca aggattcaga catcgtgatg
    22921 ggcgtaagag cgcaacaaaa agaaaaaacc cgccgttcgc tggtggaagc cgcatttagc
    22981 caattaagtg ctgaacgcag cttcaccagc ctgagtttgc gtgaagtggc gcgtgaagcg
    23041 ggcattgctc ccacctcttt ttatcggcat ttccgcgacg tagacgaact gggtctgacc
    23101 atggttgatg agagcggttt aatgctacgc caactcatgc gccaggcgcg tcagcgtatc
    23161 gccaaaggcg ggagtgtgat ccgcacctcg gtctccacat ttatggagtt catcggtaat
    23221 aatcctaacg ccttccggtt attattgcgg gaacgctccg gcacctccgc tgcgtttcgt
    23281 gccgccgttg cgcgtgaaat tcagcacttc attgcggaac ttgcggacta tctggaactc
    23341 gaaaaccata tgccgcgtgc gtttactgaa gcgcaagccg aagcaatggt gacaattgtc
    23401 ttcagtgcgg gtgccgaggc gttggacgtc ggcgtcgaac aacgtcggca attagaagag
    23461 cgactggtac tgcaactgcg aatgatttcg aaaggggctt attactggta tcgccgtgaa
    23521 caagagaaaa ccgcaattat tccgggaaat gtgaaggacg agtaatgaaa caagcaaatc
    23581 aagatagagg tacgctgctg ctggcgttag ttgctggctt atcgattaat ggtactttcg
    23641 cagcgctgtt tagctccatt gtgccatttt ctgtattccc gattatttcc ctggtgctga
    23701 cggtttactg cctgcatcaa cgttatctta atcgcactat gccggtaggc ttgccgggtc
    23761 tggcagctgc ctgttttatt ctcggcgtac tgctgtacag cacggtagtt cgtgcggaat
    23821 atccggatat cggctctaac ttcttcccgg cagtactctc cgtcattatg gtgttctgga
    23881 ttggcgcgaa gatgcgtaac cgtaagcagg aagttgctga ataatcggta ttatgccgga
    23941 tgctggcgca tccagcatgg gttttacttc gcggtcagta atacgccgca ctccatatgg
    24001 tgcgtgtagg ggaactgatc aaacagagcc agacgttcga ccttgtgcgt ctggcttaat
    24061 gtttccagat tcttgcataa cgtttccggg ttacaggaga tgtacaaaat acacggatac
    24121 gcctgcacca ttttctcggt ttcactgtcc agaccgctgc gcggtgggtc gacaaaaatg
    24181 gtttcgcact gataactctt taagtcgatc ccttgcaggc ggttaaactc gcgcacacca
    24241 ttcatcgcct gagtaaattc ttctgccgcc atacgaataa tttgtacgtt atcaatatgg
    24301 ttagctgcga tgttgtattg cgcagcagca accgacggct tagcgatttc ggtggctaat
    24361 acccgatcaa aattgcgcgc cagcgctaat gaaaagttac cgttgccgca gtacagctcc
    24421 agtaaatcgc cttttgagcc tttggttacg tccagcgccc attccagcat ctgaatattc
    24481 atcgccgcgt tcggctgggt aaagctgttt tctacctgac ggtagatcat ctctttccct
    24541 gcgaccggca gacgctcgtc gatgtaatct tgatccagct cgattttggt tttcgttgcc
    24601 cgaccaatca gatgcacatt cagattctgc gcgcgcagtg catcgcgcag ggcctccgct
    24661 tcctgacgcc actcatcatc cagcttctta tggtatagca gggaaaccac cgcctgatta
    24721 ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc gcagaacggg attattacgc
    24781 acacccgcaa tcatcgccgt catcaactgg ttgataagtt cactggcggc ggggaagcta
    24841 tccacgcgga tgcggctttt ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca
    24901 tcgtgccaga tgcggaactc cgcgcgcatc cggtaatgac tgaccggcga gcgaaacact
    24961 tccggaacca ggtcagaaaa cggtgccatc atactttgca aacgtaccac tttttcggct
    25021 aactgcgctt catactgttc tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa
    25081 ttaaagtacg cggcgattgt agggattgct catcagatgt ccagatcttg atgaattcct
    25141 atttgtgagc tacgtctgga cagtaacttg ttacaacctg tagcatccac ttgccggtcc
    25201 tgtgagttaa tagggaatcc agtgcgaatc tggagctgac gcgcagcggt aaggaaaggt
    25261 gcgatgattg cgttatgcgg acactgccat ccggtgggaa gtcatcatct cttagtatct
    25321 tagatacccc tccaagcccg aagacctgcc ggccaacgtc gcatctggtt ctcatcatcg
    25381 cgtaatattg atgaaacctg cggcatcctt cttctattgt ggatgcttta caatgattaa
    25441 aaaagcttcg ctgctgacgg cgtgttctgt cacagccttt tccgcttggg cacaggatac
    25501 cagcccggat actctcgtcg ttactgctaa ccgttttgaa cagccgcgca gcactgtgct
    25561 tgcaccaacc accgttgtga cccgtcagga tatcgaccgc tggcagtcga cctcggtcaa
    25621 tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa aacggcggtt caggtcagct
    25681 ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg ttggtgttaa ttgatggcgt
    25741 acgcctgaat ctggcggggg tgagtggttc tgccgacctt agccagttcc ctattgcgct
    25801 tgtccagcgt gttgaatata tccgtgggcc acgctccgcc gtttatggtt ccgatgcaat
    25861 aggcggggtg gtgaatatca tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg
    25921 gtggggaagc aatagttatc agaactatga tgtctctacg cagcaacaac tgggggataa
    25981 gacacgggta acgctgttgg gcgattatgc ccatactcat ggttatgatg ttgttgccta
    26041 tggtaatacc ggaacgcaag cgcagacaga taacgatggt tttttaagta aaacgcttta
    26101 tggcgcgctg gagcataact ttactgatgc ctggagcggc tttgtgcgcg gctatggcta
    26161 tgataaccgt accaattatg acgcgtatta ttctcccggt tcaccgttgc tcgatacccg
    26221 taaactctat agccaaagtt gggacgccgg gctgcgctat aacggcgaac tgattaaatc
    26281 acaactcatt accagctata gccatagcaa agattacaac tacgatcccc attatggtcg
    26341 ttatgattcg tcggcgacgc tcgatgagat gaagcaatac accgtccagt gggcaaacaa
    26401 tgtcatcgtt ggtcacggta gtattggtgc gggtgtcgac tggcagaaac agactacgac
    26461 gccgggtaca ggttatgttg agaatggata tgatcaacgt aataccggca tctatctgac
    26521 cgggctgcaa caagtcggcg attttacctt tgaaggcgca gcacgcagtg acgataactc
    26581 acagtttggt cgtcatggaa cctggcaaac cagcgccggt tgggaattca tcgaaggtta
    26641 tcgcttcatt gcttcctacg ggacatctta taaggcacca aatctggggc aactgtatgg
    26701 cttctacgga aatccgaatc tggacccgga gaaaagcaaa cagtgggaag gcgcgtttga
    26761 aggcttaacc gctggggtga actggcgtat ttccggatat cgtaacgatg tcagtgactt
    26821 gatcgattat gatgatcaca ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg
    26881 cgtcgaggcg accgccaatt ttgataccgg accactgacg catactgtga gttatgatta
    26941 tgtcgatgcg cgcaatgcga ttaccgacac gccgttgtta cgccgtgcta aacagcaggt
    27001 gaaataccag ctcgactggc agttgtatga cttcgactgg ggtattactt atcagtattt
    27061 aggcactcgc tatgataagg attactcatc ttatccttat caaaccgtta aaatgggcgg
    27121 tgtgagcttg tgggatcttg cggttgcgta tccggtcacc tctcatctga cagttcgtgg
    27181 taaaatagcc aacctgttcg acaaagatta tgagacagtc tatggctacc aaactgcagg
    27241 acgggaatac accttgtctg gcagctacac cttctgaacc acgtcccacc gtgctggtgt
    27301 ttgactccgg cgtcggtggg ttgtcggtct atgacgagat ccggcatctc ttaccggatc
    27361 tccattacat ttatgctttc gataacgtcg cttttccgta tggcgaaaaa agcgaagagt
    27421 ttattgttga gcgagtggtg gcaattgtca ccgcagtgca agaacgttat ccccttgcgc
    27481 tggctgtggt cgcttgcaac accgccagta ccgtttcact tcctgcatta cgcgaaaagt
    27541 tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa
    27601 atggcattgt cggattactg gcaacccgcg gaactgttaa acgttcttat actcatgagc
    27661 tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct gggctcggca gagatggttg
    27721 agttggctga agcgaagctt catggcgaag atgtttctct ggatgcacta aaacgtatcc
    27781 tacgcccgtg gttaagaatg aaagagccgc cagataccgt tgtattgggt tgcacccatt
    27841 tccctctact acaagaagaa ctcttacagg tgctgccaga gggaacccgg ctggtggatt
    27901 ctggcgcagc gattgctcgc agaacggcct ggctgttaga acatgaagct ccggatgcaa
    27961 aatctgccga tgcgaatatt gccttttgta tggcaatgac gccagaagct gaacaattat
    28021 tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat
    28081 ttggttgaat gttgcgcggt cagaaaatta ttttaaattt cctcttgtca ggccggaata
    28141 actccctata atgcgccacc actgacacgg aacaacggca aacacgtcgc cgggtcagcg
    28201 gggttctcct gagaatctca acagagaaaa gcaaaaaatg cttgactctg tagcgggaaa
    28261 gcgtattatg cacaccccgc gccgctgaga aaaagcgaag cgg
//
LOCUS       DAZFJF010000060        28182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFJF010000060 DAZFJF010000000
VERSION     DAZFJF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.060"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..662
                     /gene="bcsQ"
                     /locus_tag="ACHKAU_002725"
     CDS             <1..662
                     /gene="bcsQ"
                     /locus_tag="ACHKAU_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO5531752.1"
                     /translation="NVLVVDACPDNLLRLSFNVDFTHRQGWARAMLDGQDWRDAGLRY
                     TSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRYQWILIDLPRDASQIT
                     HQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINNFRIGSQVQDDIYQLWLQSQ
                     RRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWCLLNYSGLKTPVGSA
                     S"
     gene            659..3277
                     /gene="bcsA"
                     /locus_tag="ACHKAU_002726"
     CDS             659..3277
                     /gene="bcsA"
                     /locus_tag="ACHKAU_002726"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO5531753.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            3288..5627
                     /gene="bcsB"
                     /locus_tag="ACHKAU_002727"
     CDS             3288..5627
                     /gene="bcsB"
                     /locus_tag="ACHKAU_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO5531754.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5634..6740
                     /gene="bcsZ"
                     /locus_tag="ACHKAU_002728"
     CDS             5634..6740
                     /gene="bcsZ"
                     /locus_tag="ACHKAU_002728"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO5531755.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6722..10195
                     /gene="bcsC"
                     /locus_tag="ACHKAU_002729"
     CDS             6722..10195
                     /gene="bcsC"
                     /locus_tag="ACHKAU_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO5531756.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10277..12265
                     /gene="hmsP"
                     /locus_tag="ACHKAU_002730"
     CDS             10277..12265
                     /gene="hmsP"
                     /locus_tag="ACHKAU_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO5531757.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12448..13734
                     /gene="dctA"
                     /locus_tag="ACHKAU_002731"
     CDS             12448..13734
                     /gene="dctA"
                     /locus_tag="ACHKAU_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO5531758.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13954..15450
                     /locus_tag="ACHKAU_002732"
     CDS             13954..15450
                     /locus_tag="ACHKAU_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5531759.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(15546..16475)
                     /gene="kdgK"
                     /locus_tag="ACHKAU_002733"
     CDS             complement(15546..16475)
                     /gene="kdgK"
                     /locus_tag="ACHKAU_002733"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO5531760.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16707..17474
                     /gene="pdeH"
                     /locus_tag="ACHKAU_002734"
     CDS             16707..17474
                     /gene="pdeH"
                     /locus_tag="ACHKAU_002734"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO5531761.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17544..19604
                     /locus_tag="ACHKAU_002735"
     CDS             17544..19604
                     /locus_tag="ACHKAU_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5531762.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19838..21160)
                     /locus_tag="ACHKAU_002736"
     CDS             complement(19838..21160)
                     /locus_tag="ACHKAU_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5531763.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21571..22584)
                     /gene="yhjD"
                     /locus_tag="ACHKAU_002737"
     CDS             complement(21571..22584)
                     /gene="yhjD"
                     /locus_tag="ACHKAU_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO5531764.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22633..23532)
                     /locus_tag="ACHKAU_002738"
     CDS             complement(22633..23532)
                     /locus_tag="ACHKAU_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5531765.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            24052..24654
                     /locus_tag="ACHKAU_002739"
     CDS             24052..24654
                     /locus_tag="ACHKAU_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5531766.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24705..26354)
                     /locus_tag="ACHKAU_002740"
     CDS             complement(24705..26354)
                     /locus_tag="ACHKAU_002740"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5531767.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            26759..28156
                     /gene="ccp"
                     /locus_tag="ACHKAU_002741"
     CDS             26759..28156
                     /gene="ccp"
                     /locus_tag="ACHKAU_002741"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO5531768.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT         6614 a         7150 c         7827 g         6591 t
ORIGIN      
        1 aaaatgttct ggtggtcgat gcctgcccgg acaacttgtt gcgcctgtca tttaatgttg
       61 attttaccca ccgtcagggc tgggccagag cgatgctgga tggccaggac tggcgtgacg
      121 ctgggttgcg ctacacctcg cagctcgatt tgctgccttt tggtcagtta tccattgaag
      181 aacaagaaaa tccacagcac tggcaaaccc ggctgagcga tatttgctcc ggcttacagc
      241 aactaaaagc cagcgggcgt taccagtgga ttttaatcga cttaccgcgt gatgcctcgc
      301 agataaccca ccagctgctg agtttgtgcg atcactcgct ggctatcgtc aatgtggatg
      361 ccaactgcca tatccgcctg catcagcaag cgctgccgga tggcgcacat attttgatta
      421 ataacttccg tattggcagt caggttcagg acgatattta ccagctttgg ttgcaaagcc
      481 agcgccgatt gctgccgatg ctcattcatc gtgatgaagc gatggctgaa tgcctggcgg
      541 ctaagcaacc agtcggtgaa taccgcagtg atgcgctggc ggcagaagag atactgacgc
      601 tggcgaactg gtgcctgttg aactattccg ggctgaaaac gccagtcggg agtgcatcat
      661 gagtatcctg acccggtggt tgcttatccc gccggtcaac gcgcggctta tcgggcgtta
      721 tcgcgattat cgtcgccacg gtgcgtcggc tttcagcgcg acgctcggct gtttctggat
      781 gatcctggcc tggattttta ttccgctgga gcacccgcgc tggcagcgta ttcgcgcaga
      841 acataaaaac ctgtatccgc atatcaacgc ctcgcgtccg cgtccgctgg acccggtccg
      901 ttatctcatt caaacatgct ggttattgat cggtgcatcg cgcaaagaaa cgccgaaacc
      961 gcgcaggcgg gcattttcag gtctgcaaaa tattcgtgga cgttaccatc aatggatgaa
     1021 cgagctgcct gagcgcgtta gccataaaac acagcatctg gatgagaaaa aagagctcgg
     1081 tcatttgagt gccggggcgc ggcggttgat cctcggtatc atcgtcacct tctcgctgat
     1141 tctggcgtta atctgcgtta ctcagccgtt taacccgctg gcgcagttta tcttcctgat
     1201 gctgctgtgg ggggtagcgc tgatcgtacg gcggatgccg gggcgcttct cggcgctaat
     1261 gttgattgtg ctgtcgctga ccgtttcttg ccgttatatc tggtggcgtt acacctctac
     1321 gctgaactgg gacgatccgg tcagcctggt gtgcgggctt attctgctct tcgctgaaac
     1381 gtacgcgtgg attgtgctgg tgctcggcta cttccaggta gtatggccgc tgaatcgtca
     1441 gccggtgcca ttgccgaaag atatgtcgct gtggccgtcg gtggatatct ttgtcccgac
     1501 ttacaacgaa gatctcaacg tggtgaaaaa tactatttac gcctcgctgg gtatcgactg
     1561 gccgaaagac aagctgaaca tctggatcct cgatgacggc ggtagggaag agtttcgcca
     1621 gtttgcgcaa aacgtggggg tgaaatatat cgcccgtacc actcatgaac atgcgaaagc
     1681 gggcaacatc aacaatgcgc tgaaatatgc caaaggcgag ttcgtgtcga ttttcgactg
     1741 cgaccacgta ccaacgcgat cgttcctgca aatgaccatg ggctggttcc tgaaagagaa
     1801 acagctggcg atgatgcaga cgccgcacca cttcttctcg ccggacccgt ttgaacgcaa
     1861 cctggggcgt tttcgtaaaa cgccgaacga aggcacgctg ttctatggtc tggtgcagga
     1921 tggcaacgat atgtgggacg ccactttctt ctgcggttcc tgtgcagtaa ttcgtcgcaa
     1981 accattggat gaaattggcg gtattgctgt cgaaacggta accgaagatg cgcacacgtc
     2041 tctgcgcctg caccgtcgtg gctacacctc tgcatatatg cgtattccgc aggcggcggg
     2101 gctggcgacc gaaagtctgt cggcgcatat cggtcagcgt attcgctggg cacgcgggat
     2161 ggtgcaaatc ttccgtctcg ataacccgct taccggtaaa gggctgaaat tcgcccagcg
     2221 gctgtgctac gtcaacgcca tgttccactt cttgtcgggc attccacgac tgatcttcct
     2281 gactgcgccg ctggcgttcc tgctgcttca tgcctacatc atctatgcgc cagcgttgat
     2341 gatcgccctg ttcgtgctgc cgcatatgat ccatgccagc ctgaccaact ccaagatcca
     2401 gggcaaatat cgccactctt tctggagtga aatctacgaa acggtgctgg cgtggtatat
     2461 cgcaccaccg acgctggtgg cgctgattaa cccgcacaaa ggcaaattta acgtcaccgc
     2521 caaaggtgga ctggtggaag aagagtacgt cgactgggtg atctcgcggc cctacatctt
     2581 ccttgtcctg ctcaacctgg tgggcgttgc ggtaggcatc tggcgctact tctatggccc
     2641 gccaaccgag atgctcaccg tggtcgtcag tatggtgtgg gtattctaca acctgattgt
     2701 tcttggcggc gcagttgcag tatcggtaga aagcaaacag gtacgccgat cgcaccgcgt
     2761 ggagatgacg atgcccgcgg caattgcccg cgaagatggt cacctcttct cgtgtaccgt
     2821 tcaggatttc tccgacggtg gtttggggat caagatcaac ggtcaggcgc agattctgga
     2881 agggcagaaa gtgaatctgt tgcttaaacg cggtcagcag gaatacgtct tcccgaccca
     2941 ggtggcgcgc gtgatgggta atgaagttgg gctgaaatta atgccgctca ccacccagca
     3001 acatatcgat tttgtgcagt gtacgtttgc ccgtgcggat acatgggcgc tctggcagga
     3061 cagctacccg gaagataagc cgctggaaag tctgctggat attctgaagc tcggcttccg
     3121 tggctaccgc catctggcgg agtttgcgcc ttcttcggtg aagggcatat tccgtgtgct
     3181 gacttctctg gtttcctggg ttgtatcgtt tattccgcgc cgcccggagc ggagcgaaac
     3241 ggcacaacca tcggatcagg ctttggctca acaatgatga taacgcgatg aaaagaaaac
     3301 tattctggat ttgtgcagtg gctatgggga tgagtgcgtt cccctctttc atgacgcagg
     3361 cgacgccagc aacgcaacca ctgatcaatg ctgagccagc tgtagccgcc cagacggaac
     3421 aaaatccgca ggtggggcaa gtgatgccgg gcgtgcaggg cgctgatgcg ccagtcgtgg
     3481 cgcagaacgg tccttcgcgt gatgtgaagc tgacctttgc gcaaattgcg ccgccgccgg
     3541 gcagcatggt gctacgtggc attaacccga acggcagcat tgagtttggc atgcgcagcg
     3601 atgaagtggt gacgaaggcg atgctcaacc tcgaatacac gccatcgcca tcgttactgc
     3661 ctgtccagtc gcagttaaag gtttatctca atgatgagct gatgggcgtg ctgccagtga
     3721 ccaaagaaca gttgggtaaa aaaacgctgg cgcaaatgcc cattaaccca ctgtttatta
     3781 ccgacttcaa ccgtgtgcgg ctggagtttg tcggccatta tcaggacgtg tgcgaaaacc
     3841 cggccagcac cacgctttgg ctggatgttg ggcggagcag tggactggat ctgacctatc
     3901 agaccctgaa tgtgaagaat gacctgtcac acttcccggt gccattcttt gacccgcgcg
     3961 ataaccgtac taacaccttg ccgatggtct ttgcgggtgc gccggatgtt gggctgcaac
     4021 aagcctctgc cattgtcgcc tcgtggtttg gttcgcgttc tggctggcgt gggcagaact
     4081 tcccggtgct ctataaccaa ctgccggatc gtaatgccat tgtctttgca accaacgaca
     4141 aacggccgga cttcctgcgc gatcatccgg cggtaaaagc ccctgtgatt gagatgatta
     4201 accatccgca gaatccttac gtcaaactgc tggtggtgtt tggtcgtgac gacaaagacc
     4261 tgttgcaggc agcgaaaggt atcgctcagg gtaacattct gttccgtggt gaaagcgtgg
     4321 tagtgaatga agtgaaaccg ctgctaccgc gtaagccgta cgatgcgccg aactgggtac
     4381 gtaccgatcg tccggtcaca tttggcgaac tgaaaaccta tgaagaacag ttacaatcca
     4441 gcggtcttga gccagcagcg attaacgttt cgctaaacct gccgccggat ctctacctga
     4501 tgcgcagtac cggcattgat atggatatta attaccgcta caccatgccg ccggtgaaag
     4561 acagttcgcg gatggatatc agcctgaata accagttcct gcaatccttc aatctgagca
     4621 gcaaacagga ggcgaaccgc ctgctgctgc ggattccggt attacaaggt ttgctggatg
     4681 gcaaaacaga tgtctctatt ccggcgctga aactgggcgc gaccaaccag ctgcgcttcg
     4741 actttgagta tatgaacccg atgccgggcg gttcggtgga taactgtatt accttccagc
     4801 cggtgcagaa tcatgtggtg attggtgacg actccaccat cgacttctcg aagtattacc
     4861 acttcatccc gatgccggat ctacgcgcct ttgctaacgc gggcttccca ttcagccgga
     4921 tggcggatct gtcgcaaacc atcaccgtga tgccgaaagc gcctaacgaa gcacagatgg
     4981 aaacgttgct gaatactgtt ggttttatcg gcgcacagac gggcttcccg gcgattaatc
     5041 tgacggtgac cgatgatggc agcaccattc agggcaaaga tgccgacatc atgatcatcg
     5101 gtggtatccc ggacaaactg aaagacgata agcagatcga cctattggtg caggcgaccg
     5161 aaagctgggt gaaaacaccg atgcgccaga ccccgttccc cggcattgtg ccggacgaga
     5221 gcgatcgcgc ggcagaaacc cggtcaacgc tgacctcttc cggtgcgatg gcggcggtga
     5281 ttggcttcca gtcgccgtat aacgaccagc gcagcgtgat tgcgctgttg gcagatagcc
     5341 cacgcggtta tgaaatgctt aacgatgcgg tgaacgatag cggcaaacgc gccaccatgt
     5401 tcggttcggt cgcggtgatc cgcgagtccg gtatcaacag cctacgtgtt ggcgacgttt
     5461 attacgtagg tcatctgccg tggttcgagc gcgtgtggta tgcgctggca aaccatccga
     5521 ttctgctggc ggtgctggcg gctatcagtg tgatattgct ggcatgggta ctgtggcgtc
     5581 tgctgcgaat tattagtcgt cgtcgtctta acccggataa cgagtaattg aagatgaatg
     5641 tgttgcgtag tggaatcgtg acgatgctgc tgctggctgc ctttagtgtt caggcagcct
     5701 gtacctggcc tgcctgggag cagtttaaaa aggattacat cagtcaggaa gggcgcgtca
     5761 tcgaccccag cgacgcgcgc aaaatcacca cctccgaagg gcaaagttac ggcatgttct
     5821 ttgccctggc ggctaacgac cgtgcagctt tcgataatat tctcgactgg acgcagaaca
     5881 atctcgctca gggttcttta aaagaacgtt tgcccgcctg gctgtggggc aagaaagaga
     5941 acagtaagtg ggaagtgctg gacagcaatt cggcctccga tggtgatatc tggatggcct
     6001 ggtcgttgct ggaggcgggg cgtttgtgga aagagcagcg ttataccgac atcggcagcg
     6061 cgttgctaaa acgtatcgcg cgggaggaag tggtgacggt gcctgggctg ggttccatgt
     6121 tgttaccggg caaagtgggt tttgctgagg ataacagctg gcgttttaac cccagctacc
     6181 tgccgccgac gctggcgcag tatttcaccc gctttggcgc gccgtggacc acgctgcgcg
     6241 aaaccaatca acgtttattg ctggaaaccg ccccgaaagg tttttcgcca gactgggtgc
     6301 gctatgagaa agacaaaggc tggcagctaa aagccgaaaa aacattgatc agcagctacg
     6361 acgctatccg cgtttacatg tgggtaggca tgatgcctga cagcgatccg caaaaagcgc
     6421 ggatgctcaa ccggtttaaa ccgatggcga cattcactga gaaaaacggt tatccgccgg
     6481 aaaaagtgga tgtggctacg gggaaagcgc agggtaaagg accggtcggt ttttctgccg
     6541 ccatgctgcc ctttttacaa aaccgcgatg cgcaggccgt tcagcgccag cgcgtggccg
     6601 ataactttcc cggcagcgat gcctattaca actatgtgct gaccctgttt ggacaaggct
     6661 gggatcaaca ccgtttccgc ttctcgacaa aaggtgagtt attacctgac tggggccagg
     6721 aatgcgcaaa ttcacactaa acatattcac gctttccctc ggtctggccg tcatgccgat
     6781 ggtcgaggca gcaccaaccg ctcagcaaca gttgctggag caagttcggt taggcgaagc
     6841 gacccatcgt gaagatctgg tgcaacagtc gttatatcgg ctggaactta ttgatccgaa
     6901 taacccggac gtcgttgccg cccgtttccg ttctttgtta cgtcagggcg atattgatgg
     6961 cgcgcaaaaa cagctcgatc ggctgtcgca gttagcgccg agttcaaatg cgtataaatc
     7021 gtcgcggact acgatgctac tttccacgcc ggatggtcgt caggcactgc aacaggcacg
     7081 attgcaggcg acgaccggtc atgcagaaga agctgtggcg agttacaaca aactgttcaa
     7141 cggtgcgccg ccggaaggtg acattgctgt cgagtactgg agtacggtgg cgaaaattcc
     7201 ggctcgccgt ggcgaagcga ttaatcagtt aaaacgcatc aatgcggatg caccgggcaa
     7261 tacgggcctg caaaacaatc tggcgctatt gctgtttagt agcgatcgcc gtgacgaagg
     7321 ttttgccgtc ctggaacaga tggcaaaatc gaacgccggg cgcgaagggg cctctaaaat
     7381 ctggtacggg cagattaaag acatgcccgt cagtgatgcc agtgtgtcgg cgctgaaaaa
     7441 atatctctcg atctttagtg atggcgatag cgtggcggct gcgcaatcgc aactggcaga
     7501 acagcaaaaa cagctggccg atcctgcttt ccgcgctcgt gcgcaaggtt tagcggcggt
     7561 ggactctggt atggcgggta aagccattcc cgaactacaa caggcggtgc gggcgaaccc
     7621 gaaagacagt gaagctctgg gggcgctggg ccaggcgtat tctcagaaag gcgatcgcgc
     7681 caatgcagtg gcgaatctgg aaaaagccct cgcactggac ccgcacagca gcaacaacga
     7741 caaatggaac agtctgctga aagtaaaccg ctactggctg gcgatccagc agggcgatgc
     7801 tgcgctgaaa gccaataatc ctgaccgggc agaacgcctg ttccagcagg cgcgtaatgt
     7861 cgataacacc gacagttatg cagtgctggg gctgggcgat gtggcgatgg cgcgaaaaga
     7921 ttatcccgcc gccgaacgtt attatcagca gaccttgcgt atggacagcg gcaacactaa
     7981 cgccgtgcgc gggctggcaa atatttaccg ccagcaatcg ccagaaaaag ctgaagcgtt
     8041 tatcgcctcg ctctctgcca gtcagcggcg tagcattgat gatatcgaac gcagcctgca
     8101 aaacgaccgt ctggcacagc aggcagaggc actggaaaac cagggcaaat gggcgcaggc
     8161 ggcagcactt cagcggcaac gactggcgct ggaccccggc agcgtatgga ttacttaccg
     8221 actttcgcag gatctctggc aggccggaca acgcagccag gccgatacgt taatgcgcaa
     8281 tctggcgcag cagaagccga acgacccgga gcaggtttac gcttacgggc tgtacctctc
     8341 tggtcatgac caggacagag cggcgctggc gcatatcaac agcctgccgc gcgcgcagtg
     8401 gaacagcaat attcaggagc tggttaatcg actgcaaagc gatcaggtgc tggaaaccgc
     8461 taatcgcctg cgagaaagcg gcaaagaagc tgaagcggaa gcgatgctgc gtcagcaacc
     8521 accttccacg cgtattgacc tcacgctggc tgactgggcg cagcagcgac gtgattacac
     8581 cgccgcccgc gctgcatatc agaatgtcct gacgctggag ccaactaacg ccgatgccat
     8641 tctggggctg acggaagtgg atattgctgc cggtgacaca gcggcggcac gtagccagct
     8701 ggcgaaactg cccgctaccg ataacgcctc gctgaacaca cagcggcgcg tggcgctggc
     8761 gcaggcgcag cttggagata ccgcagcggc gcagcagacg tttaataagt tgatcccgca
     8821 ggcaaaatct cagccaccgt cgatggaaag cgcgatggtg ctgcgcgacg gtgcgaagtt
     8881 tgaagcgcag gcgggcgatc caaagcaggc gctggaaacc tacaaagacg ccatggtcgc
     8941 atccggtgtg actacgacgc gtccgcagga taacgacaca ttcacccgac tgacccgtaa
     9001 cgacgagaaa gatgactggc tgaaacgcgg cgtgcgcagc gatgcggcgg acctctatcg
     9061 ccagcaggat cttaacgtca ccctcgagca cgattactgg ggttcgagcg gcaccggtgg
     9121 ttactccgat ctgaaagcgc acactaccat gttgcaggtg gatgcgccgt attctgacgg
     9181 gcggatgttc tttcgcagtg atttcgtcaa tatgaacgtc ggcagtttct ccactaatgc
     9241 cgatggcaaa tgggatgaca actggggcac ctgtacatta caggactgta gcggcaaccg
     9301 cagccagtcg gattccggtg ccagcgtggc ggtcggctgg cgaaatgacg tctggagctg
     9361 ggatatcggt accacgccga tgggcttcaa cgtggtggat gtggtcggcg gcatcagtta
     9421 cagcgatgat atcgggccgc tgggttacac cgttaacgcc caccgtcggc ccatctccag
     9481 ttctttgctg gcctttggtg ggcaaaaaga ctccccgagc aataccggga aaaaatgggg
     9541 tggcgtacgt gccgacggtg tggggctaag tctgagctac gataaaggtg aagcaaacgg
     9601 cgtctgggca tcgcttagtg gcgaccagtt aaccggtaaa aatgtcgaag ataactggcg
     9661 cgtgcgctgg atgacgggct attactataa ggtcattaac cagaacaatc gccgcgtcac
     9721 aatcggcctg aacaacatga tctggcatta cgacaaagat ctgagtggct actcactcgg
     9781 tcagggcggt tactacagtc cgcaggaata cctgtcgttt gccataccgg tgatgtggcg
     9841 ggagcgcacg gaaaactggt cgtgggagct gggggcgtct ggctcgtggt cgcattcacg
     9901 caccaaaacc atgccgcgtt atccgctgat gaatctgatc ccgaccgact ggcaggaaga
     9961 agctgcgcgg caatccaacg atggcggcag cagtcagggc ttcggctaca cggcgcgggc
    10021 attacttgaa cgacgtgtta cttccaactg gtttgttggc acggcaattg atatccagca
    10081 ggcgaaagat tacgcaccca gccatttcct gctctacgta cgttattccg ccgccggatg
    10141 gcagggtgac atggatttac cgccgcagcc gctgatacct tacgccgact ggtaagtttt
    10201 cagatagcgc ctctcttaat gccgctgcga tcgggtatac tcgggcggca atctgggatt
    10261 tccgggggga gacaatttgc gcgtaagtcg ctcgttaaca atcaagcaga tggcaatggt
    10321 ggcagccgtt gtcctggtgt tcgtttttat tttttgcacc gttttgctgt tccatctggt
    10381 ccaacagaat cgctataaca cggctacgca actggaaagc attgctcgct ctgtccgcga
    10441 acccttatct tcagctattt tgaaaggcga tattcccgaa gcggaagcta ttcttgccag
    10501 cattaaaccg gcaggcgtgg tcagccgtgc cgatgtagtg ctgcctaacc agttccaggc
    10561 gctgcgtaaa agttttattc cagagcgccc ggtgccggta atggttactc gcctgtttga
    10621 gctaccggtt caaatctcgc tgggcgttta ctcgctcgaa cgtccggcaa acccgcagcc
    10681 aattgcctat ctggtactac aggcggattc cttccgtatg tataagttcg tgatgagcac
    10741 cctctcaatg ttagtgacca tttacttact tttgtcgctt atcctgaccg tcgccatcag
    10801 ctggtgcatt aaccgcctga ttttgcatcc gttacgcaat attgctcgcg aacttaacgc
    10861 catcccagcc caggagcttg ttggtcacca actggcatta ccgcgtctgc atcaggacga
    10921 tgaaatcggt atgttggtgc gcagttacaa cctcaaccag caattgctgc agcgccatta
    10981 tgaagaacag aacgaaaatg cgatgcgctt cccggtgtcg gatttgccga acaaagcctt
    11041 gctgatggag atgctggagc aggttgtcgc gcgtaaacaa accaccgcgc tgatgatcat
    11101 cacctgtgaa accctgcgtg atactgcggg cgtgctgaaa gaggcgcaac gagaaattct
    11161 gctgctgacg ctggtggaaa aactcaaatc ggtactgtcg ccacgtatga tcctcgcgca
    11221 gattagcggt tatgactttg ctgtcattgc caacggtgta caggaaccgt ggcacgcaat
    11281 caccttaggt cagcaagtgc tcactatcat gagcgagcgc ctgccgattg aacgtattca
    11341 actccgtccg cactgtagca ttggcgtggc gatgttctac ggcgatctca ccgccgaaca
    11401 gctttacagt cgcgctattt ctgcggcatt taccgctcgc cataaaggca agaatcagat
    11461 tcagttcttt gatccgcagc agatggaagc cgcccagaag cggttgacgg aagagagcga
    11521 tatccttaat gcactggaaa atcatcagtt tgctatttgg ttacagccac aggtcgagat
    11581 gaccagcgga aaactggtca gtgcggaagt gttactgcgt atccagcaac cggatggcag
    11641 ttgggacctg ccggatggct taatcgatcg cattgagtgc tgtgggctga tggttaccgt
    11701 cggtcactgg gtgctggaag agtcctgtcg attgcttgca gcctggcaag agcgcggcat
    11761 tatgctgccc ttgtcggtaa acctctctgc gctgcaactg atgcacccaa atatggtggc
    11821 ggatatgctg gaactgttaa cccgctatcg cattcagccg ggaacactga ttctggaagt
    11881 gacagaaagc cgacgtattg acgaccctca tgctgcggtg gcaatcctcc gtccgctgcg
    11941 caatgccgga gttcgggtgg cgctggatga tttcggcatg ggctacgcag ggctgcgtca
    12001 gctgcagcat atgaaatcgt tgccaatcga cgtactgaaa atcgacaaaa tgtttgttga
    12061 aggcttgccg gaagatagca gcatgattgc tgcaattatc atgctggcgc agagcctgaa
    12121 cttacaaatg attgccgaag gcgtggagac tgaagcacaa cgcgactggc tggcaaaagc
    12181 gggcgttggt attgcccagg gcttcctttt tgctcgccca ctccctattg aaatcttcga
    12241 agagagttac ctggaagaaa agtagctacc ccaaactgat tacaaaactt taaaaagtgc
    12301 tggtttgtgc gagccagctc aaacttttta acctttttgt ttcaattatg atccaggtac
    12361 atttctgtga tgttgtctgg gtgttatttt aaggccgcag gtaccccata accttacaag
    12421 acctgtggtt ttactaaagg acaccctatg aaaacctctc tgtttaaaag cctttacttt
    12481 caggtcctga cagcgatagc cattggtatt ctccttggcc atttctatcc tgaaataggc
    12541 gagcaaatga aaccgcttgg cgacggcttc gttaagctca ttaagatgat catcgctcct
    12601 gtcatctttt gtaccgtcgt aacgggcatt gcgggcatgg aaagcatgaa ggcggtcggt
    12661 cgtaccggcg cagtcgcact gctttacttt gaaattgtca gtaccatcgc gctgattatt
    12721 ggtcttatca tcgttaacgt cgtgcagcct ggtgccggaa tgaacgtcga tccggcaacg
    12781 cttgatgcga aagcggtagc ggtttacgcc gatcaggcga aagaccaggg cattgtcgcc
    12841 ttcattatgg atgtcatccc ggcgagcgtc attggcgcat ttgccagcgg taacattctg
    12901 caggtgctgc tgtttgccgt actgtttggt tttgcgctcc accgtctggg cagcaaaggc
    12961 caactgattt ttaacgtcat cgaaagtttc tcgcaggtca tcttcggcat catcaatatg
    13021 atcatgcgtc tggcgcctat tggtgcgttc ggggcaatgg cgtttaccat cggtaaatac
    13081 ggcgtcggca cactggtgca actggggcag ctgattatct gtttctacat tacctgtatc
    13141 ctgtttgtgg tgctggtatt gggttcaatc gctaaagcga ctggtttcag tatcttcaaa
    13201 tttatccgct acatccgtga agaactgctg attgtactgg ggacttcatc ttccgagtcg
    13261 gcgctgccgc gtatgctcga caagatggag aaactcggct gccgcaaatc ggtggtgggg
    13321 ctggtcatcc cgacaggcta ctcgtttaac cttgatggca catcgatata cctgacaatg
    13381 gcggcggtgt ttatcgccca ggccactaac agtcagatgg atatcgtcca ccaaatcacg
    13441 ctgttaatcg tgttgctgct ttcttctaaa ggggcggcag gggtaacggg tagtggcttt
    13501 atcgtgctgg cggcgacgct ctctgcggtg ggccatttgc cggtagcggg tctggcgctg
    13561 atcctcggta tcgaccgctt tatgtcagaa gctcgtgcgc tgactaacct ggtcggtaac
    13621 ggcgtagcga ccattgtcgt tgctaagtgg gtgaaagaac tggaccacaa aaaactggac
    13681 gatgtgctga ataatcgtgc gccggatggc aaaacgcacg aattatcctc ttaatctcat
    13741 cacttatgcc cgcactccct tccccgagtg cgggtgtttg cgcataattg acccctgtgc
    13801 ctgaacttac cacctgggtg agttcaggtt tatttcactt cttccgtgac attttcatgt
    13861 tcttgcggtc taacacgaag tgtttttaac gtcatttaga ctgaccgaca acgggcagtt
    13921 ttttattacc aggatagttg atcaggggtt cacatgcagg gcacaaaaat tcgactttta
    13981 gcgggcggtt tgctgatgat ggccactgct ggctatgtgc aggcagatgc gctccagcct
    14041 gatccagcat ggcaacaggg gacgctttcc aacggtttac agtggcaagt gctgaccacc
    14101 ccccagcgtc ccagcgatcg tgttgaaatt cgcctgctgg ttaataccgg ttcgctcgcc
    14161 gaaagtacac aacagagcgg ttacagtcac gccatccctc gtattgcgct aacgcaaagc
    14221 ggtggccttg acgcagcaca ggcgcgttca ttgtggcagc aggggatcga ccctaaacgc
    14281 ccgatgccgc cggtaattgt ctcttatgac accacgctgt ttaatctgag tttgcccaat
    14341 aaccgtaacg atttgctgaa agaagcgctc tcttatctgg caaatgccac tggcaaattg
    14401 accatcacac cagaaaccat caaccacgcg ctgcaaagtc aggacatggt ggcaacctgg
    14461 cctgccgata ctaaagaggg ctggtggcgc tatcgtctga aagggtcaac cttgttaggt
    14521 cacgatcctg ccgatccgct gaaacaaccc gttgaagcgg aaaaaattaa agatttctat
    14581 cagaaatggt acaccccgga tgcaatgacg ctactggtgg tgggaaacgt ggatgcgcgc
    14641 tcggttgtcg accaaataaa caaaacgttt ggcgaactga aaggcaaacg tgaaacgcca
    14701 gctccggtgc cgacgctttc tccgctgcgt gcggaagcgg tgagtattat gactgacgcg
    14761 gtgcgtcagg accggttatc tatcatgtgg gatacgccgt ggcagccgat tcgtgaatca
    14821 gccgcactgc tgcgctactg gcgtgcggac ctggcccgcg aggcgctgtt ctggcatgtt
    14881 cagcaagcgt taagcgccag taacagcaaa gacatcggtc ttggatttga ctgccgtgtg
    14941 ctgtatctgc gtgcgcagtg tgccatcaac atcgaatcac caaacgacaa gctgaacagc
    15001 aaccttaatc tggtggcgcg tgaactggcg aaggttcgcg ataaaggtct gccggaagaa
    15061 gagttcaatg cgttagtggc gcaaaagaaa ctggagctgc agaaactgtt tgccgcctat
    15121 gcgcgggctg ataccgatat tctgatgggt cagcggatgc gttcgttgca aaatcaggtc
    15181 gtcgatatcg cgccggagca gtatcagaaa ctgcggcagg atttccttaa cagcctgacg
    15241 gtggagatgt taaatcagga tctgcgtcag cagttgtcga atgatatggc gttaatactg
    15301 ctgcagccga aaggcgagcc ggaatttaac atgaaagcgt tgcaggcggc ctgggatcaa
    15361 atcatggccc catcgactgc tgctgccgcc acctctgtcg ccacggatga cgtacatcct
    15421 gaagtgacgg atattccacc tgcacagtaa tgcctgatgg tgatgagtta gcacaaccat
    15481 caggtattta tggatgagct ggatagaggg gtaacacttt atccagcctt ttgcatatgc
    15541 tgcgtttacg ctggcatcgc ctcacgcggg ataatcgcgc cgcgatactg aataacggta
    15601 cttgcggtca ggtgcccgcg tttcgccgcg ttttccgcac tgccgcccgt caaacgtacc
    15661 gccagataac cggcgctgaa agagtcgcca gctgcggtgg tatcgatcac tttttctttc
    15721 ggcagtttca ccgccggaac atccactaac ccttcgccag caatggacac caggcaagaa
    15781 tccgccccgc gtttcaccac cacttctttc acgcccgcgt tatgggtgcg cgcaatgacg
    15841 tcttccaccg gctgttgacc ccacagcgcg tcttcatcgt ccagcgtcag gaaggcgata
    15901 tccgtgcatt ccagcatttg ttggtacacc tgctgtgtct cttctttgct ggcccacagg
    15961 cgcggacgat agttattgtc gaaaatcact tttccgccgt tggcgcggca ttcgcgcagc
    16021 agggaaagca gcttttcgcg gctggtcggg cttaagatcg ccaggctaat cccgctcagg
    16081 tagagataat cgaaattcgc cagctcttcg caaatcgccg cagactgctc actctccagc
    16141 cagaatttgg cggcggcttc gttccgccag tagtagaacg tacgctcgcc ggtgctgtcg
    16201 gtttcaatgt agtaaaggcc cggcagacgg ttttccatcc gttgggtcag ggaagtatca
    16261 acgttctcgc cgtgccaggc gtccagcatt tgctggctaa aactgtccgt tcccagcgcc
    16321 gttacgtaat gaacggttaa tgccgcagga tcgacctgac gggcgatata gacggaagtg
    16381 ttcagggtat cgccgccgaa accgcgctta acgtccgcgc ctttctcgga aagctcaatc
    16441 atgcattcgc caatcacggc aatctttttg gacatagtcg tgaacctgat ctgtaaaaaa
    16501 ttagcgttag tttgcgctgt ggtggtttgc tggtcaacca tattaaaaca gtgttccatt
    16561 atttttttga gccagaacgt attcctgaaa gattccgttg tggagtgagg aaatttaccc
    16621 cggccaggcg gtaaagttct gcccttgcgc gccgataatc tttgtcgagt ccgggcagca
    16681 tcacttttaa acacaggaca tctttgatga taaggcaggt tatccagcga ataagcaacc
    16741 ctgaagcaag catcgagagc ttgcaggaac ggcgtttttg gttgcagtgt gagcgtgctt
    16801 acacctggca gccgatctat caaacatgcg gacggttaat ggccgtggag ctattaacgg
    16861 tggtcacgca tcccttgaac ccttcgcaac gcctgccgcc ggatcgctat tttactgaaa
    16921 tcaccgtcag ccatcggatg gaggttgtga aagagcagat tgatttgctg gcgcaaaaag
    16981 ccgacttctt tatagagcac ggcctgctgg catcggtcaa tattgatggc cctacgctca
    17041 tcgccctgcg tcagcaacca aaaatcctgc gccagattga gcgtcttccc tggctgcgtt
    17101 tcgaactggt ggagcatatc cgtctgccga aagattcaac ctttgcctcg atgtgtgaat
    17161 ttggcccgct gtggctggat gattttggta ccgggatggc aaatttctct gcactaagtg
    17221 aagtgcgtta tgactacatc aaaatcgcgc gagaactgtt tgtgatgctg cgtcagtcgc
    17281 cggaaggacg cacactcttt tctcagcttt tacatctaat gaatcgctat tgtcgcgggg
    17341 tgattgtcga gggcgtagaa acgccggaag agtggcgtga tgttcagaac tcgcccgcat
    17401 tcgccgcaca aggctggttt ctttcacgcc cggcaccgat agaaacgctg aatacggcgg
    17461 ttctggcgct ataagctgcc tcattttccg cctggctgga ctatctttag gactggcaca
    17521 ggaagaatat gaggaagcga acgatgagca aggcaggcaa aataaccgct gcgatttcag
    17581 tggctttctt gttgttgatt gtcgtggcga tcattttgat tgcaactttt gactggaatc
    17641 gactcaaacc gaccatcaac cagaaagtct ctgcggagtt gaatcgtccg ttcgctatcc
    17701 gtggcgatct gggcgtggtg tgggagcggc aaaaacaaga aactggctgg cgcagctggg
    17761 tgccgtggcc ccatgtacac gcggaagaca tcattcttgg caatccaccg gatattcccg
    17821 aagtcacgat ggtgcatttg ccacgcgtag aggcaacgct ggccccgctg gcgctgctga
    17881 ccaaaacggt ctggctgccg tggatcaagc tcgaaaagcc cgacgcgcgc ctgattcgcc
    17941 tctccgaaaa gaacaataac tggacgttta atctcgccaa cgatgataac aaagacgcga
    18001 atgcaaagcc gtcggcatgg tcgtttcggc tggataatat tcttttcgat caagggcgga
    18061 tcgccattga tgacaaagta agcaaagcgg atctggagat ttttgttgat ccgttaggca
    18121 agccgctgcc gttcagcgaa gttactggat cgaaaggtaa agcggataaa gaaaaggtgg
    18181 gcgattacgt ttttggcctg aaggcgcagg gacgttataa cggtgaaccg ctcacgggta
    18241 cgggaaaaat aggcggtatg ctggcgctgc gtggcgaagg gacgccgttt ccggtacagg
    18301 ctgatttccg ctctggtaac acccgtgttg cttttgatgg cgtcgtgaat gatccaatga
    18361 agatgggcgg tgtcgattta cggcttaaat tttctggcga ttcactgggt gatctctatg
    18421 aactgacggg cgttctgctg cccgataccc cgccgtttga aacggatggt cggctggtag
    18481 cgaaaatcga cactgaaaaa tcgtcggtct ttgattatcg cggttttaat gggcgaattg
    18541 gcgatagcga tatccacggt tctctggtct acaccaccgg aaagccacga ccaaaactgg
    18601 aaggtgatgt cgagtcgcgg caattgcggc tggcggacct gggaccgtta attggcgttg
    18661 actccgggaa aggggcagaa aagtcgaaac ggtctgaaca gaagaagggc gaaaaaagcg
    18721 ttcagcctgc gggcaaagtg ctgccttatg accgcttcga aaccgataaa tgggacgtta
    18781 tggatgccga tgttcgcttc aaagggcggc gcattgagca tggcagtagc ctgccgatta
    18841 gtgatctttc tactcatatc atcctcaaaa atgctgacct gcgcctgcaa ccgctgaaat
    18901 ttggcatggc gggcggcagc attgcggcga atattcatct ggaaggcgat aaaaagccga
    18961 tgcaggggcg ggcagatatt caggctcgtc gactgaaact gaaagaactg atgcccgatg
    19021 tggaactgat gcagaagacg ctgggggaaa tgaacggtga cgcggaacta cgcggtagcg
    19081 gtaactcggt ggcggcactt ttaggcaaca gtaacggcaa cctgaaactg ttgatgaatg
    19141 acgggctggt gagccgcaac ctgatggaga ttgttgggct gaatgtcggc aactacattg
    19201 tcggtgcgat atttggtgat gatgaggtgc gggtgaactg cgcggcggcg aatctggata
    19261 ttgccaacgg cgtggcgcgc ccgcagatct ttgctttcga tactgagaac gcgttgatta
    19321 acgttaccgg cacggcaagt tttgcttccg aacagctgga tttgactatt gatccggaga
    19381 gtaaaggaat tcggattatc acactgcgtt cgccgctgta tgtgcgtggg acgtttaaaa
    19441 atccgcaggc tggggtgaaa gccgggccgt tgattgcccg tggtgctgtt gctgcggcac
    19501 tggcaacgct ggtaacaccg gcggcggcgt tactggcact gatctcacct tccgaagggg
    19561 aggctaatca gtgtcggacg atattgtcgc agatgaagaa gtgatgaggt tggattgtgt
    19621 ttgctggtga gttcgggagc agcgtgttcc cctcacccta accctctccc caaaggggcg
    19681 aggggactgt tttgtgcgtg ttgaaacaac gagtaccgct tttccgttca ctttctgggg
    19741 gagggctgtt atgttcgcga tataacatct cgctccgctt ttctccctct ccctttcagg
    19801 gagagggctg gggtgagggt ggctgcccac acgatactta caacgactga tgtcgcgtct
    19861 catgggtcag cagcagggcg attaacgtca agccagccat cgccgccaga tataacccca
    19921 ccgcacctaa cccgtagtta gcctgcaacc aggctgcgat atatggtgca acggaagccc
    19981 cgagaatcga cgctacgttg taagagaacg atgctccggt gtaacgcact tctgtcggga
    20041 acagctctgg taacagcgcg cccatcggcc cgaaggtcag tcccatcaga cttaacccca
    20101 gcagcaggaa ggcaaaaacc agaatcgggt tgccagaacc gagcagcggg ttaaaggcga
    20161 acagcgcgaa caggatgatc agcgtggtga tgattaccat gcttttacgg cgaccaaagg
    20221 catcagccag taatccggcg actggcacca tcacgccaaa accaataact gccatcatca
    20281 gcatccacaa cacttcgtta cgcggcaggc caagcccaac tggcgcggcg gcggtactga
    20341 aggtcataga gtagaccgtc atgatgtaaa acagcgtata ggttgccagc ataatgaacg
    20401 tacccagtac ggttacgcga acatgtttgg tcagcagcgt acccagcggg atcttcacct
    20461 gttttttcgc tttagcgact ttctcaaaca ccggcgactc atgcagcgac acgcgaacat
    20521 acaggccgat aatgaccagc accgccgaga agataaatgg cacacgccag ccccagctca
    20581 taaactgctc gtcggtcagt agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga
    20641 agccaatcgg tgcgcccagc tgtgggaagg agccatacag tgcacgtttg cgcggtgggg
    20701 cgttttcagt cgccagcagc gccgcgccgc cccattcacc gcctaagccc agaccctgac
    20761 caaatcgagc cagcgccagc agcagcgggg cgaaaatacc aatcgtggca tagcccggca
    20821 gcagaccaat caccacggtc gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt
    20881 tacgcccaac gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga
    20941 aggcgatggc gaaggtggcg agcgactgta gcgttgctgc cgttggatcg ccctgcggga
    21001 agaagatatg cggaaacaca ataacggccg cagtggcgta aatgtaaaag tcgaagaact
    21061 caatggccgt gccaatgaga gaggcgacaa ggactttatt acgcgagttg atcggcgtgt
    21121 attcttgctc gtggtcgagt gttgtggctg ttgcttgcat aatcttttct tattttttgg
    21181 ctaacgaata gccatattac gcacagcaaa agtgatattt caatgtgtaa caaatcgcgg
    21241 aaatggtcaa aaaccaggca aaaaggggat aatgtttagg ccagtgattt ttcttctatg
    21301 aaatgcttca cataatttta taaatagtgg ataaaactgg tttcaggtta aataaaagtt
    21361 accaactgga tttatatgct ggggttatga gttggaatga aattgtttgc tcccaaatag
    21421 atggctgaag aggctctggg gaagagtttc aagaggttat gtgcgcatta tagccggatg
    21481 cgatgctggc gcatcttatc cggcctacgg ggagccaggc cgaataaaat cgtatgccgg
    21541 ataaggcgtt tacgccgcat ccggcctatt ttaaggctgc gttttccctg gcattcgcgg
    21601 gtcgtcttta tattcggcgg tggcaatcca cgccgcgcaa aacagcgtca aacgggcgaa
    21661 gaagtagaaa aatgccatca gccccagcac ggaaccaaat gctgcgccag agggggattt
    21721 catcagcgat ggcaaggtgt aggtcatcac gattttaatc acttcaaaac caatagcagc
    21781 cagaaatgtt ccgcgaatca gggctttttt gcgcggacgg tggcgcggca accgccagaa
    21841 gatccagaag aaaagcagat agttggcgaa gatggaaatc gccaggccaa tcaaccgcca
    21901 cgtcggtttc agccactcaa tgctgttcag gtgcagggcg ctaataatca tttgctgcgc
    21961 cgaaccggca accgaggtga tcgacagcgt cacaatcagc gcaattaaca aaccaatcag
    22021 tgaaataaaa tcacgcagat attttaccca gaacttctcc tgatcttgcg gcgagcgttc
    22081 ccagacatcg cgcgactggg cacgaatcgc ttcacgcagg ttacccatcc agttgatgcc
    22141 ggaataaagt gccaccgcca ggccgacaag ccctacagtc gtacgctgct gaacggcggt
    22201 gttgatggtg tttttcaacg tggcggctag cgtcggatcg ctgatgtttt gcagaatttt
    22261 gtcgaagata tcctgtagca acatcggatg ggaggccagc acaaagcccc ctgcggcaaa
    22321 cgacaccatc aaaatcggga tcatcgacaa aaatgagaaa taggtgatag ccgcaccaaa
    22381 ctggttaccc agccgatcgt taaagcgttc tgtcgcacga atcaggtgcg caataaccgg
    22441 ttggcgttgg actttttcgg cagtggtggt gacggtttcc agcgcctggc taactttgcc
    22501 gcctttctcg ctattatcca actgtttaat cggctcgtgc tccagatcct gggtgggacg
    22561 tttgatctcg ttttcctgcg tcatccgctc ttttgtcctt ctgtcgtggt cattcttaaa
    22621 aagtatagtc ggtcagtcca ggtgctcttt cattacgcca gccagccact gcataaacag
    22681 gtttacacgc cgggaaagct cccgacgctg cgggtaaacc agcgaaaggg agagcggctc
    22741 ggcacggtag cctggcaaca cttcaataag ccgcccggca cgcagggctt cgcgcacggc
    22801 aatgcgcggg atctgaataa tccccagacc ggcaagaccg gcggcgagat agttttcgct
    22861 actgtttacc gtcaacatgc cgccagactt aaaccactgg acgccattaa cgctggcaac
    22921 ctcaaaacct aacggatgta cacccaggtg cggtgtataa cgcactattg cgtgtgaagt
    22981 cagatcatcg gggctttgcg gataaccaaa gcgcgtcaga tagtgcggac tggcacagtt
    23041 gaccatggtc agtttgccga ggggacgggc gataacgcca tcttccggta acgcgccagt
    23101 gcgtatcacg caatcaaaac catcatgaag aatatctacc ggacggtcat ggctactcag
    23161 ttccagctca attcccggat gcagatagag aaattccgac aggcgcggca gtaacaggct
    23221 tttcgcgatt cctggcggga tgtcgatgcg taatttaccg ctgatactgg tggcatcctg
    23281 ttgaaacaga ccgtccagtt cgctgagatt actcaacaca tcttttgcgc gttgataata
    23341 ggtcatgcct tctggcgtga gtttgacccg tcgcgtggtg cgctggagaa gctgggtgcc
    23401 aagttgatgt tccagtgcct gtatctggcg cgaaacactt ccttttggca aagcaaagaa
    23461 atccgccgcg cgggaaaaac tttccagctc cgcgactttg atgaacaact gcattgcgtg
    23521 aattttatcc atttctttga ccgattgttg ttcacagcga aacagtaacg cgcatatcgc
    23581 attgtttatt gttttttcta acatctaatg ccgggtttca caatgttgtc atgacgaatg
    23641 tggcgatttc tcagcaggaa aagggcggaa tagctttctg agaaaggtga atccccctaa
    23701 acagagcgta tttttcagcg attcccttta aagtgaatag aggacgtgtt ggctatttgt
    23761 gcttattttt gtctcattgc cgtttgttat ttttgcttgt ggtcgcggag tttcattttt
    23821 aattgcatgc gtactaacca ccaatataag gaaatcctca aaattgtttt aaatagtgcc
    23881 ctcgacatgt aatttcaggc gtttagctgt aactatttat atcggtaact attttctacg
    23941 cgtcatctct gtagaatggc caccgccaaa tggcgaattc ttattttttc tcgcgggtaa
    24001 tacccttcca ctacttaagt ctttttaacg tattcaggtg gagattcagt tatgcaaata
    24061 gtcatgtttg acaggcagtc aatatttatt catggaatga aaatcagttt acagcagcgt
    24121 attccaggag tgagtattca gggggccagt caggcagacg agttatggca aaagctggaa
    24181 agttaccctg aagccttagt tatgctcgat ggcgatcagg atggtgagtt ttgctactgg
    24241 ttgctgcaaa aaaccgtggt gcagtttcct gaggttaagg tgttaatcac ggcgacggat
    24301 tgcaataaac ggtggttaca ggaagttatc cattttaatg tgctggccat tgtgcctcgt
    24361 gattcaaccg tcgagacatt tgcgttggcg gtaaatagtg cggcgatggg gatgatgttt
    24421 ctgcctggag actggcggac gacgccggaa aaggacatta aagatctcaa atcgttaagc
    24481 gcccgccagc gggagatttt aaccatgtta gcggcgggcg aatcaaataa ggagatcggc
    24541 agagcgctga atatcagtac cggaacggta aaagcgcatc tggaatccct gtatcgccgt
    24601 ctggaagtga aaaatcgcac ccaggcggca atgatgttaa atatctcctc ctgaagagga
    24661 taattttaca gccagcagga agttagcggc tggctgtaaa aatattatgg ttcgccgtac
    24721 aaaccaatta aacggcgtac cacaccgtta gtccagccaa acccatcctg caacggatac
    24781 tcgccaccgc cgccttcgcg gggaacacca tcggcaatat ggtatttctc gatcattttg
    24841 tgctgttcca gatagaactg attcaccgtt ttcagccagc tgcgcgcgat ttcatcaccc
    24901 agaaggtcat cgccatacat tttaaatccc tgaattgcca tccattgtaa cggtgcccag
    24961 ccattgggtt tatcccactg ttcaccggtt tcgtactcgc ttgccagaat cccgccaggt
    25021 gtcagtaacc ggctgcgtac ggcgtttgcc agacgatcgg cctgttcatg gttcgccatg
    25081 ccgacataga gcggcacaat ggcggcagcg gaaaacagcg ccagttgttc gcgtcgccag
    25141 tcgtaatcgc ggtagatgcc gttttcatca tcccagaggt aacggtttac cgcatcgcga
    25201 cgggcactgg ccttctggcg gaacagcgct tctgtctctt tctcgccttt cagcgccgag
    25261 atgttggcga tggcgctctc cagtttaaac aggaaggcat tcagatcgat ggggatgaac
    25321 tgggtggtac gaatgctcgc cagacgacca gtatcacgca gccaacggga agagtaatcc
    25381 caacctgagg ccgctcccgc gcgtaaatcg cggtacacct cgttgggcgg gcgaccagaa
    25441 tgtttcgcgg tttcaacgtc ctcaagccag gattcgtcac gcggcgtgtc gcgatcgtcc
    25501 caataacggt tgagcagcga tccgtccggc atccgcacaa catggcgata ggcctgatta
    25561 gggatcaacg attctgcacc gtccatccag aaggcatatt ccattttaag gtggtcgaga
    25621 tagcggcgcg caccgcgtac accatcttct tcaaacaact ccaccatcag cgcaaaaacc
    25681 ggtggttgcg atcggctcaa ataataggtg cggttgccgt tggggatgtg accatagttt
    25741 tcgatcatcc aggcgaagtt atcggccatg catttcagca aatcttcccg accactttcc
    25801 gccagcccca gcatggtgaa ataggaatcc caatagtagg tttcgctaaa acggccgccc
    25861 gggacaatat atgactgcgg tagcgccagc agagaagacc acggaatgtg atcctgtggt
    25921 tcgcgggtta gcaccggcca cagctggtcg atatgctctt tcagggaatt ttgcgggtcc
    25981 gatacatact cgctggagta gacctccggc agccagaagt gattttcaac aaacttgcgc
    26041 aagtcaaaat cacgatgacg gcgcacttta cggtagcgga ttaaaatatc cagcgggtcc
    26101 attttgggtg cgcagtcggg aaaggtttta ctgtcgggga aaattttcgc cgactgaaca
    26161 tgctcgaaca gttcgagata gcgatcggca ggcgtcagcg catcagaggc gggcagccct
    26221 tcaatcatct cgggttccgg ttctgcctcg atcatctcat ccagttttaa ttcatacggg
    26281 tccagttcat agcagagatc gacctcgatc atcagttcgt ctggattagg gttttgaatt
    26341 ttctgattga gcataaggat ccaacctccg aaattcgtcg ctaattatcg ggcttttctt
    26401 taagggtagt cattcgctcc gcctggtggg cgacaaagcg tgcggtggat cacgtttatt
    26461 aacttgtcaa aaaatataac gcactaatat caatggaatt tataaaaata cggatttcgt
    26521 gttgtttttc attttcagaa cattcaatcc tgctctcgcc gcaatcatga tatgccattc
    26581 ctgatctggg taataggcac aggctatctt attgatagtt tatattcatg taattgattg
    26641 attcctatca aaaagcgtat ctccctgttt tttatgatgg ctttgtctcg cagagacgtt
    26701 tgcagcattc tggaaaattt tctgggtcac aataactcac caccgtgagg gaagaataat
    26761 gaaaatggtc tcacgtatta ccgcgatcgg cctggctggc gtcgcgattt gctatttagg
    26821 gttatctggt tatgtgtggt accacgataa taaacgcagt aaacaggccg atgttcaggc
    26881 atctgctgtc agtgaaaata ataaggtttt aggctttctc cgcgaaaaag gatgcgacta
    26941 ttgccacacg ccttcggcag aattacccgc ctattattat attcctggcg cgaaacagtt
    27001 gatggattac gacattaagc ttggatataa atcttttaac ctcgaggccg tgcgtgcggc
    27061 actgctggct gataaacccg tttcgcaaag cgatctgaat aagattgaat gggtgatgca
    27121 gtatgaaact atgccaccaa cgcgttatac cgcgctacac tgggcgggta aggtgagtga
    27181 tgaagagcgg gcggaaatac tggcctggat tgcaaaacag cgcgcggaat attacgccag
    27241 caatgatact gctccggagc atcgcaatga accggtgcag cccatcccgc aaaaactgcc
    27301 taccgatgcg caaaaagtgg cgttgggctt tgcgctgtat cacgatcccc gtttatcggc
    27361 tgatagcacc atttcatgcg ctcattgcca tgcgttgaat gcggggggcg tcgatggcag
    27421 aaaaacatcg attggtgttg gtggcgcagt tgggccgatt aacgcgccga cggtatttaa
    27481 ctcagtattt aacgttgagc agttctggga tggtcgtgcg gcaacattgc aggatcaggc
    27541 aggtggaccg ccgttgaacc cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc
    27601 taagctggaa aaagatccgc agcttaaagc gcagttcctc gaagtttatc cgcaaggttt
    27661 cagtggcgaa aatattactg atgccattgc tgaatttgag aaaacattaa ttacgccgga
    27721 ttccccattt gataaatggt tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa
    27781 aggctatcaa ttatttaaag ataataaatg tgcaacttgt catggtggta ttattctcgg
    27841 cggacgttcc tttgaaccgt tggggctgaa aaaagacttt aactttgggg aaattacggc
    27901 ggcggatatt ggtcgtatga atgtgactaa agaagagcgt gataaattgc gtcagaaagt
    27961 acccggttta cgtaacgttg ctttaacggc accgtacttc catcgcggtg acgtgccgac
    28021 gctggacggg gcggtgaaac tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga
    28081 ggatgtggat gatatcgtag ctttcctgca cagtctgaac ggggtgtaca cgccgtatat
    28141 gcaggataaa caataattaa tttgatcgcc cgaacaggaa tg
//
LOCUS       DAZFJF010000061        27323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFJF010000061 DAZFJF010000000
VERSION     DAZFJF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.061"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..449)
                     /gene="mutT"
                     /locus_tag="ACHKAU_002742"
     CDS             complement(60..449)
                     /gene="mutT"
                     /locus_tag="ACHKAU_002742"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO5531769.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(509..3214)
                     /gene="secA"
                     /locus_tag="ACHKAU_002743"
     CDS             complement(509..3214)
                     /gene="secA"
                     /locus_tag="ACHKAU_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO5531770.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3276..3788)
                     /gene="secM"
                     /locus_tag="ACHKAU_002744"
     CDS             complement(3276..3788)
                     /gene="secM"
                     /locus_tag="ACHKAU_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO5531771.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4019..4936)
                     /gene="lpxC"
                     /locus_tag="ACHKAU_002745"
     CDS             complement(4019..4936)
                     /gene="lpxC"
                     /locus_tag="ACHKAU_002745"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO5531772.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5037..6188)
                     /gene="ftsZ"
                     /locus_tag="ACHKAU_002746"
     CDS             complement(5037..6188)
                     /gene="ftsZ"
                     /locus_tag="ACHKAU_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5531773.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6249..7511)
                     /gene="ftsA"
                     /locus_tag="ACHKAU_002747"
     CDS             complement(6249..7511)
                     /gene="ftsA"
                     /locus_tag="ACHKAU_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO5531774.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7508..8338)
                     /gene="ftsQ"
                     /locus_tag="ACHKAU_002748"
     CDS             complement(7508..8338)
                     /gene="ftsQ"
                     /locus_tag="ACHKAU_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO5531775.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            complement(8340..9260)
                     /gene="ddlB"
                     /locus_tag="ACHKAU_002749"
     CDS             complement(8340..9260)
                     /gene="ddlB"
                     /locus_tag="ACHKAU_002749"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5531776.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSYGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9253..10728)
                     /gene="murC"
                     /locus_tag="ACHKAU_002750"
     CDS             complement(9253..10728)
                     /gene="murC"
                     /locus_tag="ACHKAU_002750"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO5531777.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10782..11849)
                     /gene="murG"
                     /locus_tag="ACHKAU_002751"
     CDS             complement(10782..11849)
                     /gene="murG"
                     /locus_tag="ACHKAU_002751"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO5531778.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11846..13090)
                     /gene="ftsW"
                     /locus_tag="ACHKAU_002752"
     CDS             complement(11846..13090)
                     /gene="ftsW"
                     /locus_tag="ACHKAU_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO5531779.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13090..14406)
                     /gene="murD"
                     /locus_tag="ACHKAU_002753"
     CDS             complement(13090..14406)
                     /gene="murD"
                     /locus_tag="ACHKAU_002753"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO5531780.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14409..15491)
                     /gene="mraY"
                     /locus_tag="ACHKAU_002754"
     CDS             complement(14409..15491)
                     /gene="mraY"
                     /locus_tag="ACHKAU_002754"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO5531781.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15485..16843)
                     /gene="murF"
                     /locus_tag="ACHKAU_002755"
     CDS             complement(15485..16843)
                     /gene="murF"
                     /locus_tag="ACHKAU_002755"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO5531782.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16840..18327)
                     /gene="murE"
                     /locus_tag="ACHKAU_002756"
     CDS             complement(16840..18327)
                     /gene="murE"
                     /locus_tag="ACHKAU_002756"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO5531783.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18314..20080)
                     /gene="ftsI"
                     /locus_tag="ACHKAU_002757"
     CDS             complement(18314..20080)
                     /gene="ftsI"
                     /locus_tag="ACHKAU_002757"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO5531784.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20096..20461)
                     /gene="ftsL"
                     /locus_tag="ACHKAU_002758"
     CDS             complement(20096..20461)
                     /gene="ftsL"
                     /locus_tag="ACHKAU_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO5531785.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20458..21399)
                     /gene="rsmH"
                     /locus_tag="ACHKAU_002759"
     CDS             complement(20458..21399)
                     /gene="rsmH"
                     /locus_tag="ACHKAU_002759"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO5531786.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21401..21859)
                     /gene="mraZ"
                     /locus_tag="ACHKAU_002760"
     CDS             complement(21401..21859)
                     /gene="mraZ"
                     /locus_tag="ACHKAU_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO5531787.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22461..23465)
                     /gene="cra"
                     /locus_tag="ACHKAU_002761"
     CDS             complement(22461..23465)
                     /gene="cra"
                     /locus_tag="ACHKAU_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO5531788.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23547..23633)
                     /locus_tag="ACHKAU_002762"
     CDS             complement(23547..23633)
                     /locus_tag="ACHKAU_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO5531789.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23645..24136)
                     /gene="ilvN"
                     /locus_tag="ACHKAU_002763"
     CDS             complement(23645..24136)
                     /gene="ilvN"
                     /locus_tag="ACHKAU_002763"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5531790.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24139..25863)
                     /gene="ilvI"
                     /locus_tag="ACHKAU_002764"
     CDS             complement(24139..25863)
                     /gene="ilvI"
                     /locus_tag="ACHKAU_002764"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO5531791.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25982..26026
                     /locus_tag="ACHKAU_002765"
     CDS             25982..26026
                     /locus_tag="ACHKAU_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531792.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26181..27125)
                     /gene="leuO"
                     /locus_tag="ACHKAU_002766"
     CDS             complement(26181..27125)
                     /gene="leuO"
                     /locus_tag="ACHKAU_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO5531793.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         6531 a         7782 c         6559 g         6451 t
ORIGIN      
        1 cagcgtcgca tcaggcatct tgtgcaaatg tcggatgcgg cgtgaacgcc ttatccgacc
       61 tacagacgct taagcttcgc aattaccggt tcattggctg gcggaaaatc atcggcatta
      121 agaccgacta gcgacatcca ctcaccgggt tgcccttctt taccccacgg ctccccttcc
      181 cagctttcga ccagccagaa ccacagagtt atatgcctgt ccgggaattc atattccagt
      241 ttttcaaata gcgaaaaatg ttgtggggta atcccgactt cttcctgaag ttcacgcacc
      301 accgcctgtt ccggcgtttc gcccatttca attttaccgc cgggaaactc cagtttattc
      361 gccatgtgcg catctgctgc gcgacgcgtt ataaagattt cattgttctc gttgcgaata
      421 atacctaccg caatttgcag ctttttcatt gtcttaaacc tataaaaaag gcgcagaatc
      481 ctgcgccttt tacttcaact gttagctttt attgcaggcg accatggcac tgcttgtatt
      541 ttttaccaga accgcacggg caaggatcgt tacgtcctac tttgcgctca ccggtttgcg
      601 ccgccagtgc agctgcggct gcagagtcgt catcctgatg gctaagctgc tgcatttgcg
      661 ctaaacgctc ggcttccata cgacgctgtt gttccagctc ctcaacctct tcaggcatac
      721 gtacctggac tttgctcagc gtgctgataa cttcatattt taacgactcc agcatcgctg
      781 caaacatgga gaacgattca cgtttgtatt cctgcttcgg atctttctgt gcatagccac
      841 gcaggtggat accctgacgc agatagtcca tcgctgccag gtgctctttc cacagggagt
      901 caagcgtttg cagcatgacg cctttctcga agtgacgcat catctcagca ccaaccactt
      961 cttctttacg ctgatacact tcgatggact gcgccagaat gcgctcacgc agcgtctctt
     1021 catgcagttc tggttcttta tccagccact cggcaattgg taaatcgagg tcgaaatcgt
     1081 tcttcagacg ttcctgcagc cccggaatat cccacatttc ttccagcgac tgtggcggaa
     1141 tgtaggcatc aatggtggct ttgaacacat cttcacgaat gctgttgatg gtttcgctca
     1201 catcgctgac atccagcagt tcgttacgct gggagtaaat ggcgcgacgc tgatcgttag
     1261 ccacgtcatc atattccagc agttgcttac gaatgtcgaa gttacgactt tcaactttac
     1321 gctgggcgtt ggcaatcgct ttagtcaccc acgggtgttc aatggcttcg cctggcttca
     1381 tacccagttt acgcatcatg ccggatactc ggtcggaagc aaaaatacgc atcagcgcat
     1441 cttccatcga caggtagaaa cgggaagaac cagcatcccc ctgacgacca gaacgaccgc
     1501 gcaactggtt atcgatacga cgggattcgt gacgctcggt accgatgata tgcaggccac
     1561 ctgcttccag taccgcatcg tgacgtacct gccagtcggc tttaattttt tcaatttgct
     1621 ctacggtcgg attttccagc gcggcaactt ctgcctgcca gctaccaccg agcacaatat
     1681 ctgtaccacg acccgccata ttggtcgcga tagtcaccgc agccggataa cctgcctgag
     1741 caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag gacgttgtgc ttaataccgg
     1801 ctttggtcag ttcgtttgac accagctccg atttttcgat ggagatagta cccaccagca
     1861 ccggctggcc tttcgcagta cgttctttga tatcttcaat gatcgcctga attttttccg
     1921 cttcagtcat gtagaccagg tccggcagat ctttacgaat cattggacgg ttggtcggaa
     1981 caacgacggt atccagcttg tagatggagc taaattcgaa agcttcggta tcagcagtac
     2041 cggtcatccc cgccagtttt tcatacagac ggaagtagtt ctggaaggtg atcgaagcca
     2101 gcgtttggtt ttcgttctgg atctgcacac cttctttcgc ttccacagcc tggtgcagac
     2161 catcggacca gcgacggccc tgcatggtac gaccggtgtg ttcgtcaacg atgataactt
     2221 caccatcttt aacgatgtag tcgacgtcgc gggtaaacag cgcatgagcg cgcagcgccg
     2281 ccgttacgtg gtgcatcagc atgatgttgg ccggagagta cagagactcc ccttcatcca
     2341 tgatgccctc tttcaccagc agttcttcaa tcagcaccag accacgttcg gtcaggttca
     2401 cctggcgaga tttttcgtcc accgagaagt ggccttcgcc ctggaaggtt tcggagtctt
     2461 ctttttcctg acggatcagg tgcggaataa ttttattcac gcgtttatac atttccgagc
     2521 tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc ttcatcgatc aggatggagt
     2581 ccacttcgtc caccagcgca tagtgcagtt tacgctgtac acgttcttca gggctgaacg
     2641 ccatattgtc gcgcaggtag tcaaagccgt attcgttgtt cgtaccgtaa gtgatgtcag
     2701 ctgcgtaagc ttcgcgcttt gccggtgctg gcatgcccgg caggttgata ccgacagtca
     2761 ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc acgttgcgcc aggtagtcgt
     2821 tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt caggtaagca ggcagcgttg
     2881 cggtcagggt tttaccttca ccggtacgca tttcggcgat gcagcgttcg ttaagaacca
     2941 taccgccgag taactgaacg tcgaagtgac gcataccaaa gacgcgctta cttgcctcac
     3001 gtaccacggc gaaagcttcc gggatcagat tttccagcac ttcgcctttt tccagacgtg
     3061 cacgaaactc tgcggttttc cctttcagtt cttcgtcgga gagtttttcc atctccggtt
     3121 ccatggcatt gatgatgttg accactttgc gcatccggcg cagggtgcga tcgttacgac
     3181 taccgaaaac tttagttaac aatttgatta gcataataaa atctcaaacg ccccgcgttg
     3241 cggagttaat aaaatgaagt aaaggtttat tgttgttagg tgaggcgttg agggccagca
     3301 cggatgcctt gcgcctggct tatccagacg ggcgtgctga attttgcttg tggggtaaaa
     3361 tgcgcataat caatgcgata acccttttca gacggcgtgc cttcctgggt cagcagcgcg
     3421 ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag gcaaagattc ttcagcaacg
     3481 ggcagtgttt gcggtgccat tgcgaaagaa agatgacgga ttaccgtgcg aatggcatgt
     3541 tgatgccagt aatcaacgga atagttcgaa ttcgggcggc gtgtgttcgc ttccagcaag
     3601 gccaattgac caaagttaac tttggctgaa ggctcgtggt tgcgggttgt cgcttttgcg
     3661 ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac ctaaactcgc cgcaaccatc
     3721 cctaataaga gatgcggcca gaagtagcgt ttaccaaact gtcgccagcg cgtcagtatt
     3781 ccactcacgt tattgccatc ccgatcgtat tcaaacgtat ttagcgcaca aatcttatca
     3841 ttaaagcctg cagtccacag cacgaatgaa gataagttgt gcggaaaagt gcttaagaaa
     3901 acagcgttcg caccgttttt tatagctctt ataaaattta aaaaaaaacg actggttcac
     3961 ctggccggag agagtgccag atttgccagt cgaattttat acgacagtat aaatgtcgtt
     4021 atgccagtac agctgaaggc gctttgaagg ccaacggcag ttctgcgtcg tcctggaagg
     4081 tcacatattc ccaggcttcc tgtttcgcca ggacagcctg cagcagtttg ttattcagtg
     4141 catgaccgga tttataagcg gtaaatgcac caataatatt gtgaccacac atgaacaagt
     4201 caccgatcgc atcgagcatt ttgtgacgca caaattcgtc ttcaaaacgc aggccgtctt
     4261 cgttcagtac gcgataatcg tcaacaacga tggcacaatc gaagctgccg cccaggcaca
     4321 aaccacggga ctgcagatat tcgatatcac gcatgaaacc gaacgtacgc gcacggctga
     4381 tctggcgcat aaacgcatca gcggagaagt tcatcgcata gcgctggttg ctggaatcaa
     4441 tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa accattgtac ggcttaaatt
     4501 cagcccactt atcgccatct tcgacacgaa cagtctcttt gatgcgaaca aattttttgg
     4561 cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta tacaaacgga gcggcgctgc
     4621 cgtccatgat cgggatttcc ggcgcgttaa cttcgataac aatgttatcg atgcccaagc
     4681 ccgcgagagc agcattgagg tgctctacgg ttgaaatccg tacatcatgc tcgttgacca
     4741 gacacgtaca gagcatggta tcacgcacag atttggcatc ggccgggaaa tctaccggtg
     4801 gattcaagtc ggtgcgacga tagatgaccc cggtgttggc cggcgcaggg cgtaacgtca
     4861 gggtgacttt cttgccggta tgtaaaccga cacccgtcgc ctgaacgata cgtttaagtg
     4921 tcctttgttt gatcatcgta ttatctcgcc aaattaccta tccaaccgaa gtgtactata
     4981 cattcggcgg gccagtttag cacaaagagc ctcgaaaccc aaattccagt caattcttaa
     5041 tcagcttgct tacgcaggaa tgctgggata tccagataat ccggctcttt cgcagtttgc
     5101 ggcgcattgt cattcacgac tttagcaacc ggcttctgct cctgggtcag cggagccatc
     5161 ccatgctgct ggtagcgatc catcactggc tgctgaacct gcttattggt caccagagtg
     5221 atttcaggac gtttgtccat gccgatacct gtcgcaacaa cggttacgcg cagctcgtca
     5281 ttcatatccg ggtcaagaga agtaccgata accacagtcg cgttgtcgga agcaaatgca
     5341 cggatggtgt tacctaccgt ttcgaactca tccagacgca ggtcgaagcc cgccgtgatg
     5401 ttaaccagca cgccgcgcgc gccagacagg tcgatatctt ccagcagcgg agaagagata
     5461 gccatttcag cagcttcttc cgcacggtct tcaccgctcg ccacgccaga acccatcatt
     5521 gcgtagccca tctcagacat tacggtgcgt acgtctgcaa agtccacgtt catcaaaccc
     5581 ggacgagtaa tcagttcagc gataccttgc acagcgcctt tcagtacatc gttcgctgcg
     5641 ccaaacgcat ccagcaggga gataccgcgg cccagaactt tcagcagttt gtcgttcggg
     5701 atagtgatca gagagtccac atgcttggac agttcagtga tcccctgctc cgcgaatgcc
     5761 atacgcttct tgccttcaaa gttgaaaggc ttagtgacga cagcaacggt caggataccc
     5821 aaatcttttg ccacttcagc gacgactggt gctgcacctg taccggtacc accacccata
     5881 cccgcagcaa taaagaccat gtctgcacct tccagcgccg cacgcaatgc atcgcgatcc
     5941 tcatcagccg cattgcggcc aacttctgga ttagcgccag cgcccagtcc tttggtgata
     6001 ccgctaccga tttgaatcgt ctgtccaacc gctgttttac gcagcgcttg tgcatcggta
     6061 tttaccgcga agaattcaac accttcaatg cgctcgcgca ccatgtgttc aacagcatta
     6121 ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt cattggtaag ttccattggt
     6181 tcaaacatag tttctctccg atttgtgcct gtcgcctgag gccgtaatca tcgtcggcct
     6241 cataaaaatt aaaactcttt tcgcagccaa ctattgagtc gcttgatcca cgagccaact
     6301 gatgctgtaa cacgtttttc tacttcagct tcaccgttaa gatgtgactc tttcccatag
     6361 tgaagcaatc ccaccgccgt cgaataatac ggctcctgag cataatccgt taaaccggta
     6421 atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca cgcgctgagc acaggctgca
     6481 agaccttcga tctgcgctgc gccaccggtt aatacaatgc ctgccgccag gtgatgttta
     6541 accccttgtt ggcgaagctt ttcctgcaac tgcaatatct cttcgttgac caggttgagc
     6601 agctcggtat agcgcggctc gatcacctct gccagggtct gacgttgcag actccgcggc
     6661 ggacgaccac ctacgctcgg cacttccacg ctctcatctt ttccaacgat ggaacccagc
     6721 gcacaaccgt ggcgaacttt aatcgcttcg gcgtcgcttg gcggcgtgcc aaaggcgtaa
     6781 gcgatatcac tggtcacgac attgccagca taaggaatta ccttagtgtg gcgcaatgcc
     6841 ccaccggtat aaacggcgat atccattgta ccaccaccga tatcgacgac gcagacaccc
     6901 agttcacgtt catcttccgt caataccgaa taacttgatg ccagtccggc aaatatcagt
     6961 tggtcaactt tcagcccaca acgttcaacc gctttgacga tgtttttcgc catatcgttg
     7021 tgacatgtga tcaggtgcac ttttgcctgc atccgcacgc ccgaaagtcc taccggattc
     7081 ttgatccctt cctgatagtc aatcgcatac tcttgcggga tcacatgcag cacacgatgc
     7141 tcatcgcgca cacgcaccga tttcgcggta tggacgacgt tttccacatc ttcttgcgtc
     7201 acttcttctt cagaaatagg caccatacca atttcattct ggcagctgat gtgcttacca
     7261 gaaagcgcca gatataccga agagatctga caatctgcca tcaattctgc ctggtcaatg
     7321 gcgcgttgta cgcacttgac cacggattcg aggtcgttca ccccgccttt atccatacca
     7381 cgtgacgggc agctgcccac gccaatgata ttgaccatac cgtcgggcag aacttcccct
     7441 actaaagcgg caaccttcgc ggtaccaatc tccagtccta ctaccagttt tctgtccgtc
     7501 gccttgatca ttgttgttct gcctgtgcat gattttgttg ctgagtagat tcctctggcg
     7561 gcaagggcgc ccagcctact gccgctccag agtcataacg caaatcaacg tagctaatcc
     7621 gtttgccatc ggtttgcgcc tgctgctgta aaaccggata aagttctaca aagcgagcca
     7681 aacgtttcat cgtatcgccc cggccaagat tgagcttaat atcgttattc agcgtcaact
     7741 gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt aaatctgtcc tttgccagca
     7801 tctgccccat ttcgcgatag ccctgcaaca cttcattggc gctgccttcc gggccataca
     7861 gcattggaag cacctgcttg ctggtgcgat ctggcggcac gctgaaggtg tttccttccg
     7921 cgtctaccat atgttgatca ttccaccgcg caatcggcac atattcaacc agatgaatct
     7981 tcaattcatc aggccactgc tttctgacgc tcacctgctt aatccacggc aggcgttgtt
     8041 ctatttgcgt ctggatgatg ttgacatctt gggtcataaa ggtacccggc tcacccaatg
     8101 ccaggatcga ctgccggata tcgtcattac gcgtgtaatg gcgttcaccg gtcaacacca
     8161 gctttgagag cggcaggcgt tgcgcatctt ccatccagcc caacacgacc cagccgctca
     8221 ccaacactgt cgttaaaacg gtcagcagga aaaggatccc cgccagacgc gttccattat
     8281 tgcggcgaga agaaacctct tcttcgctat ttcgcgtgtt cagagcagcc tgcgacatat
     8341 tagtccgcca gttccagaat tcgtactacc aactgcgaga agctcatacc tgcctgacgt
     8401 gccgccatcg gcaccaggct gtggctggtc atacccggtg aggtattggc ttccagcaga
     8461 taaaactgtc catagctgtc cagcataacg tcaatacgtc cccatccttt gcaacctaac
     8521 gtcgtccatg ctttcagcac taatgcctgc aaattggcct cttgtgacgc ttccagacct
     8581 gcggggcaga aatactgtgt ctcatcagag agatacttcg cctcataatc atagaaggtt
     8641 ccggacggtt gaatacgtat tgacggtaaa atttcttcac cgagtatcgc aaccgtgaac
     8701 tccggcccac ttagccattt ttcaatcaat acttcttcat cgtgctgaaa tgccaatctt
     8761 aatgcatctt gtagagcatt ttctgctact acttttgaca ttcccacact ggaaccttcg
     8821 cggctcggct taacgataac cggcaaaccc agagcagaaa tttctgctaa ctgcttatcg
     8881 ctcaggcctt tttcaaactc tgcgcgggtt aacgctaccc acggcgcgac cggtaaaccg
     8941 gcaccttgcc acagcagttt gctgcgtagt ttatccattg aaagcgcaga tgccatcact
     9001 ccgctgccgg tataaggcaa gcccatcagc tcgagcatgc cctgcagcgt accatcttca
     9061 ccgccgcgac cgtgtagcgc gataaacact ttctgaaaac ccatcgactt cagttgcgtc
     9121 acgtcgactt ctttcgggtc gacaggatac gcatcaatac cgccttcacg cagcccggct
     9181 aacaccgctg cgccagaatt cagagaaact tcccgctcag cggaagtccc gcccaacagg
     9241 accgcgattt tatcagtcat gttgttcttc ctccggagtt tgcggcttca gtttgatttc
     9301 agctaaagaa cgggcaattt ttccgatatt accagccccc tgaacgagaa tcaggtcgtt
     9361 accggttaat accggtgcca gcatctcggc tacctgcgca gggtccggca ccagaatggg
     9421 atcaattttc ccacgtccac gaattgtgcg acacagcgaa cggctgtccg ctcccggaat
     9481 tggcgcttcg ccagccggat acacttccag catcaacagg gtatcaacct gcgtcagcac
     9541 attggcgaaa tcatcataca ggtcgcgcgt acgggtaaaa cggtgcggct gaaacagcat
     9601 taccaggttt ttatccggcc agcctgcgcg cgccgcttta atggtggcgt caacttccgt
     9661 cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg cttttaccat tcactggctc
     9721 cagcgggaat tcaccgagga aatcaaaacg gcgcccagtc ccctggaagc tctccagcgc
     9781 acgtaaaata gcctcgtcat caattccctc ttccgtagca accgcaaccg cagctgcggc
     9841 gttcagcgcg ttatgacgac ctggcgcatt cagggtgacg cgcatcggct ctttgtcctg
     9901 gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga taatcttcta cacgcacgtc
     9961 ggcatcttcg ctgaagccgt aagtcgtggt ctgacgtccc actcgcggta acaattcgcg
    10021 gatcaccgga tcatcaacac acatcaccgc acgaccgtaa aacggcaggt tgtgcagaaa
    10081 attaataaaa gtctgtttta aattctcaaa gtcgccctgg taggtatcca tgtggtcggc
    10141 ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc aggaacgatg catcactctc
    10201 atctgcttcg gcaatcaggt accgaccatg ccccaaacgc gcatgaaccc ccgccgcttt
    10261 taccagcccg ccgttaacga aggttgggtc gagccccgct tctgcgtaga tgctggaaac
    10321 catcgcggtg gttgtcgttt tgccgtgcgt tcctgcaatg gcgatgccat gacgaaaacg
    10381 cattaactca gccagcattt cggcacgacg gatcaccgga atacgcgctt catgagctgc
    10441 gacaatttcc gggttatagg cagaaatggc gctggaaaca acgaccacgc tggcatcacg
    10501 tacgttttcc gggcgatggt tgaaataaat cgtcgctccc agattcatta actgctgcgt
    10561 gaccggattt ggcgctaaat cggaaccact gatctgataa ccttcattgg ccagaacttc
    10621 ggcaataccg cccataccgg caccaccgat gccgacaaaa tgtatgtgcc gaacgcgacg
    10681 catttcgggc acgatggaac gcagttttgc caattgttgt gtattcattc tttacgccat
    10741 taacttctta aattcatacg atgcaaaagg catcgctaca attacgcccg ggcagcccgg
    10801 ctcacttcat ttgccactcg ctcggtggca tccggaatgg atgcagcgcg ggcgcgttct
    10861 gccatggtta ataaggtttc tcgcgaccac ccggccaggg tgttggcgac agcatccacg
    10921 ctaagctgtg gctgctcgat aattttggct gcgcccgctt tttccagcgg tagcgcattc
    10981 cagtattgct ggcggtcttt atgttgaaac ggcacaaaca acgccggtag tcctgccgcg
    11041 gcgatttcac tcaccgttaa agcaccggag cggcaaacga cgacatccgc ccacgcatac
    11101 gccgccgcca tatcatcaat aaattccgtc actttatgct gcggttgccc cgcttcggca
    11161 tacgcctgtt caacggattg ttgcgaacct ttaccgctct gatgccagat agtgactgaa
    11221 tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa gaatgcgtgc gccctgggaa
    11281 ccacccacta ccagcacacg aaccggacct tcacgtccag ccaaacgttg ctgcggcaac
    11341 ggcagcgcca gtacatcggt acgcaccggg ttacccacca cttccgcatt agggaaagcg
    11401 cctggaaacg cctgcatcac tttggtggca atcttcgcca gccatttatt ggttaagccc
    11461 gcaataccgt tttgttcatg aagtacaacc ggaatgccta acgaccacgc ggccagacca
    11521 cctggacctg atacatagcc gcccataccg agtaccacgt caggtttgta cgctttcata
    11581 atcgcccgcg cctgacgcca ggcgttgaag atacgcagcg gcgcagctat cagtgctttt
    11641 ataccttttc cacgcagacc agagatacga atgaaatcaa tttcgatgcc atgttttggc
    11701 actaagtccg cttccatacg gtcggcagtc cccagccagc gaacttgcca accctgagcc
    11761 attagatggt gcgcaaccgc cagtcccggg aatacatgtc caccggttcc gcctgccatc
    11821 accattaatc gctttccttg accactcatc gtgaacctcg tacaaacgcc tgcgctttct
    11881 ccagacgcgt ttcataatca atacgcaaca gcatcatgat ggctgtcgac ataatcagta
    11941 agctcgaacc accgtaactg atcagcggca atgtcagacc tttggtcggt aacatccccg
    12001 ccgccgcgcc tacgttaacc agcgcctgga agctaaacca gatgccaata gaacaggcga
    12061 gaaaaccgga aaaacggtgg tcaatttcta acgctttgcg gccaatcgac atcgcgcgaa
    12121 aagcgacgaa gaataccatt aaaagtgcca gcaccacacc gacatacccc agttcttcgc
    12181 cgataatggc gaaaataaag tcagtgtgcg cttccggcag atactccagt ttttgtaccg
    12241 agttacctaa accttgtccc caaagttcgc cgcgaccaaa cgccatcagc gattgcgtta
    12301 actgatagcc gctgccaaag ggatcttccc acgggttcca gaatgcggta acacggcgga
    12361 tacggtacgg ttcggcgagt atcagcaaca caaccgctga aatgcccata ccgataatgg
    12421 caatgaactg ccacaatttc gctcccgcca ggaacaacat cgccagcgta gtcacaaaca
    12481 acaccaccac cgtaccaagg tctggctgtg ccagcagtaa cactgccaac accagaatca
    12541 cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac ttcgtcgcct ttacgcacca
    12601 gatagttggc gatataacaa aacagcgaca gttttgtcag ctccgcaggc tggatacgca
    12661 gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga gctacccact accagcacga
    12721 tcatcagcag gatgatagat ccgagcagca tcgtggcact gtagcgttgc cagaactcca
    12781 tcggcagacg cagcgtaatg atcgccagaa tgaatgccag gatcagatag acgccatcac
    12841 gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc tatgggcatt gacgccgagg
    12901 tcaccatgat aaagccaatc gccgcaaggc cgaaggtcag ccacagtaag gtgcgatcgt
    12961 acatgatcag gctgtcggta tctttttccc gcgagcccat cacccagcct tttagcgccg
    13021 tggagatcca gaccaggata ctgaatcctg gcaggcgcgg cattttcagg cgagggagag
    13081 ataaacgcat caacctaact ccttcgccag acgggcaaac tcattgcctc gttgttcaaa
    13141 gttcttgaac tgatcaaggc tggcacaggc cggggagagc agaaccatat cgcccggctg
    13201 aacacgcgga gccagcaagc gcatcgcctg ttccatagtt tcggtttgtt ctgccacttc
    13261 cgggcgtagc gccgccagct gcgcaccgtc acgaccaaaa cagtacaaac gcacgttgtc
    13321 gccattcaga tagcgcgcca gtggactgaa gtccgccgat ttaccatcgc cgcccagcaa
    13381 caaatgcagc gtgccgtcta cctgcaagcc attcaacgcc gcttccgtac tgccgacgtt
    13441 agtggctttc gaatcgttaa tccagcgtac gccattgtgc tccagcacca cttcaaagcg
    13501 atgcggcaga ccggtgaagg tggttaacgc tttcaggctg ctggcgcgcg gcaaaccagc
    13561 tgcgtctgcc agtgccagtg ctgccagcgc attggtgtag ttatgctgcc cggaaagttt
    13621 catctctttc acgttcagca ctttctcgcc tttcactcgc agccaggttt ctccctgctg
    13681 atgattcagg tgatagtcac ccatgttgac gccaaagctg acgcagcgtt catcagcacc
    13741 gcgaatcggc attgttaagg catcatcagc attgacgaca caaactttcg cattttcgta
    13801 aatacgcaat tttgccgcac ggtattgctg caaaccaaac ggatagcggt ccatatgatc
    13861 ttcagtcacg ttcagaatgg tcgccgccac cgcctgtaag ctggaagtgg tttccagctg
    13921 gaagctcgac agttccagca cgtacagttc gcactcagca tcaagcagca tcaacgctgg
    13981 caggccaata ttgccaccca caccaacgtt aacccccgcc gctttcgcca tttcacccac
    14041 tagcgtggtg accgtgcttt tgccgttaga accggtaatc gccacaatcg gtgcttgtgc
    14101 ttcgcggcag aacagctcga tatcgccaac gatttcgatt ccggcatcag cggcagcgct
    14161 taaggatgga tgcgccagtg caataccggg actggcgaca atcagatctg ccgccatcag
    14221 ccattcatca ttcagactgc ccgtgtggcg ttctacggct tcgggtaatt tatccaggcc
    14281 aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca ccgcgagcga ggaaaaagtc
    14341 cacgcaggaa agcccagtga ggcccaggcc gataatgacg acatttttac cctgataatc
    14401 agccatgatt aacgtacctt cagcgttgcc agaccaatca gaaccagcat cagcgaaata
    14461 atccagaaac gcacaatgac gcgcggttcc ggccagcctt tcagttcata gtggtgatga
    14521 atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa aggagccgac ctgcaggatg
    14581 acagaaagcg tttctaccac gaacacgccc cccataatca ccagcaggaa ttcctgacgt
    14641 agcagtacgg cgataatgcc taacgcacca cctaacgcca gcgaacctac atcgcccata
    14701 aagacctgcg ccggataggt gttaaaccac aggaagccca gtcctgcccc gactatcgcc
    14761 gtgcagacaa tgaccagttc cccggcgtgt cgcagatacg gtatgtgtaa atagctggca
    14821 aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac caccggcgac aaatacggtc
    14881 ggcataattg ccaggccgtc gagaccatcg gtcaggttta ccgcgttgcc agtacccaca
    14941 atgacgaagt aagccagcag aatgtagaac agccccagct gcggcatcac atctttaaag
    15001 aatgggacca ccagctgcgt tgcgggcgtg tctttgccgg caaggtacag ggcgaaggcg
    15061 atacccagcg caatgaccga catccagaaa tacttccaac gagcgatcaa ccctttggtg
    15121 tctttacgca ccactttgcg ataatcatca acaaagccaa taacaccgta acctaccagc
    15181 accaccaaca cgcaccagac gtacggattg gacgggtaag cccacagcag tacggagatc
    15241 acaatcgccg tcaggatcat aatcccgccc atggtcggcg taccgcgctt gctgaagtgt
    15301 gattcaggac cgtcgttacg caccacctga ccaaaggaaa gtttttgcaa atgagcaatc
    15361 atacgcgggc ccatccacaa tgagatgaac agcgcggtca gcaggctgac gatggcgcga
    15421 aacgtcagat aggaaaagac gttaaagccg gaataatatt tgaccaaatg ttcggccagc
    15481 caaactaaca tgtcccattc tcctgtaaag cgcgtactac ctcttccatg gcggcactac
    15541 gtgaaccctt aactaaaatc gtaattacct gttgctcagc aatcagtgat ttaagacgcg
    15601 taattaacgc agttttatca gcaaaatgtt cgccaacgcc gctggcggtg ctgatagcat
    15661 ggctttgttt acccacgctt aacacgcggt caataccagc agcttttgcc gcctcgccca
    15721 cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc catatcgccc accaccagca
    15781 cgcggtagcc cggcatttca gccagtacct gaacagcggc ggtcattgaa ccgacattgg
    15841 cgttgtagga gtcgtcgagc agcaactggt tttctgccag ttggatgggg aacagacggc
    15901 ctggaacagc tttcagattt gccagccccg ctttgatagc atcaagcgtt gcgcccacgg
    15961 acatggagag cgcagcggct gccagcgcat tcgcaatatt gtgacgcccc ggcaacggca
    16021 gcagaacatc gacgctaccg gttggggttt gtagggtaaa ttccgtaccg tgcgaggtca
    16081 catggatatt ggtggcggtg aaatcgctgt tggcggcatt gggtgagaaa cgccacactt
    16141 tgcgtgagcc aattacgctc tgccagttca gccagtcgtt gttgtcggcg ttcataatgg
    16201 cgataccgtt ttccggcagg ccgctaaaga tttcaccttt cgctttcgcg acacccgcaa
    16261 gcgagccaaa accttccaga tgcgccgctg ccaggttgtt gaccagcgca gcttccgggc
    16321 gagtcagact cacagtccag gctatttcgc cctgatggtt cgcgccaagt tcaataactg
    16381 cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg tacaccgatg tcgttgttga
    16441 gattgcctgc cgtataaagc gtgttgccgc actggcttaa aatcgccgcc gtcatctctt
    16501 taacggaggt tttgccggag gaccccgtca gagcaaccac gcgcgccgga acttgctggc
    16561 gaacccatgc agccagttca ccaaacgcca gacgcgtatc cttgacgatt aactgcggca
    16621 ggtcgatgtc cagcggacgg ctaaccagta gtgcgcctgc gccgccagct ttcgcctggt
    16681 cggcaaaatc gtgggcatca aaacgttcgc ctttcagggc aacaaacagg cagcccggcg
    16741 tcagttttcg ggtatcagtg gttacagcat caagggtgat atctgcacct tgcagttcac
    16801 cgttgagaat atcggtaagt tggctaaggg ttacgctaat catgcaatca cccccagcag
    16861 acgcgccacc gtgacgcgat cggagtagtc cagacgctga ttgccaacaa tctggtaatc
    16921 ctcatggcct ttgcccgcga ccagtaccac atcattctct ttagcctgca taacggcgca
    16981 agtcaccgct tcagcacggc cttccatcac tttggcatgt ccggcatcta acattcccgc
    17041 cagaatatcg ttgatgatgg cacgcggttc ttcggtacgc gggttatcgt ccgtcaccac
    17101 cgccacgtca gcaaactctt cggcaattgc gcccatcagt ggacgcttac ctttatcgcg
    17161 atcgccacca cagccaaaga cgcaccacag cttgcccgca cagtgcaggc gcgccgcctg
    17221 taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc accaccaccg tcggtttgcc
    17281 tggcgcagtg aacacttcca tacgtccgca aaccggttgc agacgcgcgg cggtttttaa
    17341 cagatcagcc agcgggtagc caagtgccag cagtgtcgcc agcgcgagca gcaggttgct
    17401 gacgttaaaa gcgcccatca gatggctttc aatttcgcca tcgccccaac ttgaactaaa
    17461 gcgaatggtc gcgccgctgt cgtgatagtt cacttcggtc gctttcaacc agcgtccgtg
    17521 acagttcgga ttgatatgat cttccattga taccgcaacc gcgtccggca gttttgccag
    17581 ccagcggcgg cccacttcat cgtcggcgtt aacaatcgcc tgaccacaat gatgttcaga
    17641 ataaagcagc catttcgcgg cttcgtagtg ttccatatca ccatgataat caaggtgatc
    17701 gcggcttaag ttggtaaaga ccgacgccgc aaatttcaat gccgccacac ggtgctgtac
    17761 cagcccgtgg gaggaaactt ccattgcgca aaacgtcgcg ccctgatcca ccagccccgc
    17821 cagctcatgc tgaacatcga ctgccgaacc ggtggtattt tctgtcggga tcactttccc
    17881 cagcagaccg ttaccaacgg tgcccattac cgcgctggtt tcgccaagca gttggctcca
    17941 ctgcgccagc agctgggtag tcgtggtttt gccgttggtg cccgttacgc ctacgagacg
    18001 taaattgtca gagggttcat ggtaaaagcg gcccgccagt gcagataaac gctcgttgag
    18061 ctggctgaga tagatgaccg gtacgccgtg catttcacgg atttcaccgt cggtcgcctc
    18121 acctttcgcc tctgcaataa tggcagtcac accttgcgct atcgcctgcg ggatatatcg
    18181 acgcccgtcc gcctgatgac ctactacagc tacaaagaga tcgcccgccg cagccacacg
    18241 gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg tctggcaccc acggagcaag
    18301 aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc ttgattaatc acaaattcat
    18361 ttttatcgcc cgttgtcagc gcatccggct cgatgttcat ggtacgcaat acgccgccca
    18421 tgatggcacc aaagaccggc gctgaaacgg cgccgccgta gtatttaccc gcctgcggat
    18481 cgttgataac aacaaccagc gcgaagcgcg gctgactcgc aggcgcaacg cctgcggtat
    18541 aagcaatata tttattgatg tagcgaccgt ccggcccgac ctttttcgcg gtaccggttt
    18601 taatggcgat acgatagcct ttaatcgccg ccttcacgcc gccgccgcct ggtagcgcca
    18661 cgctttccat catatgcacc acagtgcgga caatggattc cgggaagaca cgttcaccgg
    18721 gaaccggggg gtcaactttg gtaatcgaca gtgggcgata aatgccgtag ctgccgatag
    18781 ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag cccgtagccg aaagagaagg
    18841 tggccctctc tatgtcagac caccgttgtt tttgaggata taagccactg cgttctccga
    18901 ccaaccccaa attggtcgct tttcccagtc caaaacgtga gtaagtatct actaacgctg
    18961 aggacggcat cgctaacgcc agcttggaaa caccgacgtt actcgacttc tgtaataccc
    19021 cggtcagggt taattcgctg tagcgtgcca cgtctttgat ttcgtggccg ttaattcgat
    19081 aaggaatggt attgagtacc gagttttccc gcaccacgcc acgttgcaac gcggtcatta
    19141 ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc ggtgatggta cggttacgca
    19201 tcgcctcttt cggcgtgccg ctcagattgt tagggttgta tgacgggctg ttagccatcg
    19261 ccagcacttc accggtgttg acatccacca gcacggcgct accagattca gccttgttaa
    19321 aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg caggcgttca tcaatactca
    19381 gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat atcttcaatt acgcgaccat
    19441 agcggtcttt acgcacaatg cgctcacccg gctgcccggt aagccattta tcgaaactct
    19501 tctcaacgcc ctcaatccct tgactatcga cgttagtaaa gccgatgagg tgagcagtca
    19561 cttcgccgga cggatagtaa cggcgagact cttcacgcag atgaatcccc ggcagtttca
    19621 gttttttgat gtagtccgcc atgtcagggt tcacctgacg cgccagataa ataaagcgcc
    19681 ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag cggaatattg agcgcgttag
    19741 ccagcgcctt ccagcggtca ccgacgctga taccgccagc gtcatgcact tctttcgggt
    19801 cagcccaaat cgcttttacc ggcacgctca ccgctaacgg gcgaccagaa cggtcagtaa
    19861 tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga acgcatgtcg ccctctttca
    19921 ccagcatatc cggggagata acttgtaacc acgctacgcg tccgagcaga aaagccagcg
    19981 ccaggagaat acagccgcat aacaacgcaa aacgccaact gataaagttg gcatgttctt
    20041 cctgacgttt tggtttctgc gttttcgccg ctgctttcat gtgtcgcgtt tatccttatt
    20101 tttgcactac gatattttct tgtgacggat caacatgctg catttgcagc ttttccgtgg
    20161 cgatcctttc cacccggcta tggtcgccga gcgcattctc ttcaaggatc aggttgcgcc
    20221 attcaatgtc taaagcatct cgctccagca ccagttgttc gcgctgagcg gtcagtaaac
    20281 gggtatggtg cgccgtggtt accacagtca ccgccgtcaa aataatgcaa atgaacaggc
    20341 agagtggcag cttcccaaat cgcaaaagat cgtcaccgat aacaccaggc aatgcatggc
    20401 gctcgtggct tcccatcgat cctttaactt tgcttagagc ttctgtcact ctgctgatca
    20461 tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg gcacgagggt tctcagccac
    20521 ctcttcttcg cccggcatta acttgcctag tgctcgcagc tgacggccac ccagtttttt
    20581 gagctgctct tcagtcatcg gtaaccctgc cggaacttgc ggaccgcggc tgttttcacg
    20641 cataaaacgt ttcacaatac ggtcttccag cgagtggaag ctgatgatcg aaagccgccc
    20701 acccggggcc agcacgttga gcgagctttt tagcgcctgc tctatctcct ccagttcact
    20761 gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg ggatgtttaa acttatcttt
    20821 caccggcgtt gcagcagcca cgacttccgc cagttctttg gtgcgggtca tcggctgttc
    20881 gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca aaacgctctt caccataggt
    20941 tttcaatacc caggcgatat cggcttcttc tgcggtttgt agccattcag cggctgactg
    21001 cccacgggtt gggtccatac gcatgtccag cggaccatcg cgcataaagg aaaagccgcg
    21061 ctcagcatca tcaagttgcg gtgaagagac gccaagatcg aggagaatgc cgtcgatctt
    21121 gccgataaga tcgcgctcgg caacgtattc gcccagcgcg gagaaaggtc cgtggatgat
    21181 ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata gcctgcgggt cgcgatcgat
    21241 cgccagcaaa cgcccctctt cgccaagctg cgagaggatc agacgtgagt gaccaccgcg
    21301 accaaaagtc ccatcaatgt agatgccatc aggacggata ttgaggccat taacggcttc
    21361 atccagcagc accgtagtat gtttatagtt ttccatcatt ttatagagac aagtcctgca
    21421 gtcgctccga taagtctccg gtagccaact gctctgcgtc gatatcttcc ttgacctgtt
    21481 gatgccaggt tgtttcatcc cacagctcaa acttgttgaa ctgtccaacc agcatcactt
    21541 cttttgtcag cccggcatgt tgccgcagta ctggcgcgat taacaatcga cctgcgccat
    21601 ccatctgaca ttcgctggca tgacctaaca gtaggcgctg cacacggcgc tcaaccgggt
    21661 tcatgctcga cagacgcgat aatttttgct cgataatttc ccattcaggt agggggtaaa
    21721 gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat ttgaccggca gcgttctcaa
    21781 gcagctgttc ccggtaacgg gtaggcactg ataagcgccc tttgctgtcg agattgacta
    21841 acgttgctcc ccggaacatg ccagcctcac cccttattcc cactttgccc cacaatttcc
    21901 cactgaaagg agtttacgga gcggaggaaa agcttgtcaa gccagtcaca aggcataccg
    21961 aggtaaaaaa cccgatgttt acggctaata tcagcgttgt ttattttacg tccaacgtgc
    22021 gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc ctcatcgtca tcaccgtttt
    22081 aaaatgactc actattttcc acagcaaata taaagtgtca gtttgcgacg cgagcggcat
    22141 tttaggacat atcttccccg gttaacagtc cctgttgcgt ctgtgtggcg cttgatgcca
    22201 gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc ggcaattcat ctctgcatgt
    22261 aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat tattgtgaat ttcagctggt
    22321 gagttaatta acgggcgggt ttaaaagaac ttatcaataa tagtcaggaa tagtcttatt
    22381 tactttaagg atattgatgt ccattcccgt actctacgcg ccagagggaa attcacctgg
    22441 cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac cgcggcgata gagattacgt
    22501 ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt ccaggcttgc caggacaatc
    22561 tccagcacac gctctgcgac atcgcggtga cgttgagcca ctgcaagcac cgggcactgt
    22621 aagaagtcga gcagttcgtt atcgccaaag gtggcaattg ccaggtcaga aggcagttta
    22681 ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca acgcaaacga cgttgtgaac
    22741 agcgcctgcg gcatcggatg cgtttccagc catttttcga ataactgggc agccgcctcc
    22801 cgctcatagc tgttggcata caggaaatgc acttcgcgcg gatcatcttt ccaggcagta
    22861 cggaaacctt gttcacgcag gaagctgaca gaaagctccg gcagcgcgcc aagataaagc
    22921 accgtctcgg cgggaaactt acgtaactct tccgccagca tttcggcatc atcctgatcg
    22981 gcaccaacca cgctggtgaa atgttcacga tcgagggcgc ggtccagcgc gacaatcggg
    23041 aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag gtggcaacga cgtcgaaaca
    23101 ataatggcat caacctgacg ctgtaaaagg tgctcaatgc agcgcatttc gttgtctggc
    23161 tgatcttctg agcaggcaat cagcagttga taaccccgtt gccgcgcctg gcgctcaaga
    23221 tagttagcga tgcgggtata gctggtgttc tccagatcgg ggatcacaag accaatagaa
    23281 cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg ggtggtaatt gtgctcacgc
    23341 accacagcca tgactttttc aacggttttg tcgctcacac ggtattgctt cgctttgccg
    23401 ttaataacat agcttgcagt ggtccgcgac actcccgcca gccgagcgat ttcatccagt
    23461 ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc attgtactgg tatgggttaa
    23521 attcattaac atctaagcgc agaaaattaa ctgcggcaac tgcttttatt ccaggttccc
    23581 actgatttcg caaaaaaagc ccaacgtgac atgttgggct gcaaattgcg cactgagatc
    23641 attctcaacg cattatttta tcgccgcgcg aaagtccgac cacaccagag cgagcaactt
    23701 ccacaatttt cgccacatcg cgaatcgatg ctaaaaatgc atcaagctta tcgctggtgc
    23761 ctgctaattg aacggtataa agcgaggggg tgacatcgat aatttgccca cggaatattt
    23821 ccgtattacg tttcacttcg tcacgcccgt aaccgctggc ctgaattttc accagcatga
    23881 tttcccgctc aacatgcgcg ccctgcccca actcactcac gcgcaagaca tcgaccagct
    23941 tgtgtaattg cttttcgatc tgctcaagca ctttttcatc gcccacggtc tggatggtca
    24001 tacgcgataa tgtcggatcg tcggttggcg caacggtcag gctttcaatg ttgtagccac
    24061 gctgggaaaa aaggccaatc acgcgggata acgcgcctga ttcattttcg agtaagactg
    24121 ataatatccg gcgcataatc aggttctctc cgttttgctt aaccacattt catccattcc
    24181 gcccccgcga atctgcatcg ggtagacgtg ctcactgcca tcgacggtaa catcaacaaa
    24241 caccaggcga ttattgcaca cctgttccag cgcctcgcta agtttgcttt ccagctcatg
    24301 cggatgagag atctggatcc cgacgtgacc ataggcttcc gccagacgga cgaaatcggg
    24361 tagtgattgc atataagatt gtgaatgacg gccggaatag atcatgtcct gccactgttt
    24421 caccatcccc agatagcggt tattgagatt caccaccagt accggcaact cgtattgcaa
    24481 cgcggtagac agttcctgga tgttcatctg aatactgccg tcgccagtga cgcaaaccac
    24541 ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca ggtaaaccaa aacccatcgt
    24601 gccgaggcca ccggaattga tccagcgacg cggtttgtcg aatggataat aaagtgcagc
    24661 aaacatctgg tgctgcccga catcggacgt cacgtaagcg tctcccttcg tcaaccgcca
    24721 aagagtctcg atcaccgcct gcggtttaat cttttcactg tgagtgtcat atttcaggca
    24781 ctgacgagcg cgccactgtt caatttgctg ccaccagtcg cggatctcat ccagtggttg
    24841 atgggcggat tcttgcgaca agagttcaag catttgttcg aggacctggc gagcatcccc
    24901 cacaatcggg atatccgcag tcacggtttt agaaatggaa gtaggatcaa tatcgatatg
    24961 cagaacagtg gcatttgggc agtactttgc cagattgttc gtcgttcggt catcaaatcg
    25021 taccccgacg gcgaaaatca catccgcgtt atgcatcgtc atattggctt cgtaggtacc
    25081 gtgcattccc agcatgccca gtgcctgacg atgcgttgcc ggaaacgccc ccagccccat
    25141 caatgaggaa acaacgggca gattcaacgc ctccaccgtt tctttcaact gctgatggca
    25201 gcccgccatg attgccccac cgcctacgta gacaaccggt ttttttgccg ctaccagcgt
    25261 ttgcagagcg cgcttaattt gccctttatg tccggtagta gtgggattgt aagaacgcat
    25321 actgaccgac tcaggccaga catagggtaa tttgttcgcc ggattaagaa tatctttcgg
    25381 taaatcaacg accactggtc ctgggcgacc acttgccgcc agccagaaag cctttttcag
    25441 cacctgcgga atgtcttccg tttgcttaac cagaaaactg tgtttaacca ccggtcgcga
    25501 aatccccacc atgtcgcact cctgaaaggc atcgtaacct atcaacgagg tcgctacctg
    25561 cccggaaagg acaactaatg gaatggaatc catataagcg gtggcgatgc cggtaatcgc
    25621 attggtcgcc cctggacccg acgttaccag cacgacgccg acttccccgg tcgcgcgcgc
    25681 cagaccatcg gccatatgca ctgccgcctg ctcatgacga actaaaacat gatcgatacc
    25741 acccacggta tgcaatgcat cgtaaatatc aaggactgcg cctccgggat aaccgaatac
    25801 ttgtttaacg ccctgatcga taagcgatcg gacgaccatc tcggctccag acaacatctc
    25861 catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa cacattaacc gtccgatctt
    25921 gcttaagcaa ttggcagcca gagacataaa cgcagggtaa taagaataaa caggaggaaa
    25981 ggtgaaaaga aacgataaaa tcctccatta cgaaggatta aattgataat aaatcggctg
    26041 aatcccacaa cttacagcaa tttctctatt tttttaatta atcctaaaac agagagccca
    26101 cacattcaga ataaaattct acattcaata aaaaatacag aataaaccag acattcatgc
    26161 ctgacctatt ctgcattcag ttagcgtttg caaattgaga ctaattgctc ttccatccac
    26221 tgatggcctt tatcgcgccc ggcagcttca tgccaggaga gataacaggt tctgctattt
    26281 tgttttaacg gcagcggtaa tacctgtaat tctaaggatt cagcgaactc ttcagccagc
    26341 caacgcggcg caatagcgac caaatgcgtt tgcgacacca cgctaagtac gctcatcatt
    26401 gccatgccct gatacgcgat actggcttgc ttatctaccg tgtcatacca aggttgacta
    26461 aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt cgttataaac atcatgtttc
    26521 agtaacgggc ccttaattgt tggatgattt ttgctggcta ccagcaccat ttcatcttta
    26581 aataatggca cgctggtaaa ttcaggacga tggaagtctt cataactaat cacaaactcc
    26641 gtttcctgat aacgcagctg atgttcagtg ttctgattta atgaagactt gaacataaca
    26701 tgtatatttg gcgcaatctg ctcaatgtga ttataaatct gcgaggtcag aatgctgtct
    26761 aacgggctgc aaacacaaag atgaaataca cgttcactgc tcgcgggttc aaaacctgaa
    26821 ccaggcaatt cattttgtac tagttgcaat gcctgacgaa ctgagccaaa aagttgaaat
    26881 gcgcgagcag tcggttgaat accacggcca taacgaacaa aaagctcgtc attaaacatc
    26941 accttcaggc gtgcaacagc gttactgacc gcaggttgcg acatacccag aacatgagcg
    27001 gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa cggttaataa gttgagatcg
    27061 accatgcgta gctgtggctt gcttaactcc gccgtctctg aatgatctgt ttgtacctct
    27121 ggcatactta actccactgt cacacttaac tccctttccc ttattggaat gcagagaaat
    27181 aatcctgaag aatatgattt atcatgcata taaaataaga aaaagcaaaa tgagtaaaat
    27241 tcggaaaaca taaagatacg ggttctgata aaaacagaat ccattgccgt ggaatgagtc
    27301 atttaccaca gcataataat cca
//
LOCUS       DAZFJF010000062        26758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFJF010000062 DAZFJF010000000
VERSION     DAZFJF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.062"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(17..799)
                     /gene="yjjV"
                     /locus_tag="ACHKAU_002767"
     CDS             complement(17..799)
                     /gene="yjjV"
                     /locus_tag="ACHKAU_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5531794.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(796..1869)
                     /gene="yjjU"
                     /locus_tag="ACHKAU_002768"
     CDS             complement(796..1869)
                     /gene="yjjU"
                     /locus_tag="ACHKAU_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO5531795.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(1991..2152)
                     /gene="ytjA"
                     /locus_tag="ACHKAU_002769"
     CDS             complement(1991..2152)
                     /gene="ytjA"
                     /locus_tag="ACHKAU_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO5531796.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(2279..2884)
                     /gene="osmY"
                     /locus_tag="ACHKAU_002770"
     CDS             complement(2279..2884)
                     /gene="osmY"
                     /locus_tag="ACHKAU_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO5531797.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(3277..4866)
                     /gene="prfC"
                     /locus_tag="ACHKAU_002771"
     CDS             complement(3277..4866)
                     /gene="prfC"
                     /locus_tag="ACHKAU_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO5531798.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVSVATARWVECADAKKFEEFKRKNESQLALDGADNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(4957..5634)
                     /gene="yjjG"
                     /locus_tag="ACHKAU_002772"
     CDS             complement(4957..5634)
                     /gene="yjjG"
                     /locus_tag="ACHKAU_002772"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO5531799.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(5649..6095)
                     /gene="rimI"
                     /locus_tag="ACHKAU_002773"
     CDS             complement(5649..6095)
                     /gene="rimI"
                     /locus_tag="ACHKAU_002773"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5531800.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(6064..6477)
                     /gene="holD"
                     /locus_tag="ACHKAU_002774"
     CDS             complement(6064..6477)
                     /gene="holD"
                     /locus_tag="ACHKAU_002774"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO5531801.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            6580..7611
                     /gene="rsmC"
                     /locus_tag="ACHKAU_002775"
     CDS             6580..7611
                     /gene="rsmC"
                     /locus_tag="ACHKAU_002775"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO5531802.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNNGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            7912..7998
                     /locus_tag="ACHKAU_002776"
     tRNA            7912..7998
                     /locus_tag="ACHKAU_002776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7946..7948,aa:Leu,seq:cag)
     gene            complement(8037..8273)
                     /gene="yjjZ"
                     /locus_tag="ACHKAU_002777"
     CDS             complement(8037..8273)
                     /gene="yjjZ"
                     /locus_tag="ACHKAU_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO5531803.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
     gene            8414..9202
                     /gene="fhuF"
                     /locus_tag="ACHKAU_002778"
     CDS             8414..9202
                     /gene="fhuF"
                     /locus_tag="ACHKAU_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO5531804.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(9240..9917)
                     /gene="bglJ"
                     /locus_tag="ACHKAU_002779"
     CDS             complement(9240..9917)
                     /gene="bglJ"
                     /locus_tag="ACHKAU_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO5531805.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(9875..10600)
                     /gene="yjjQ"
                     /locus_tag="ACHKAU_002780"
     CDS             complement(9875..10600)
                     /gene="yjjQ"
                     /locus_tag="ACHKAU_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5531806.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            11219..11989
                     /gene="yjjP"
                     /locus_tag="ACHKAU_002781"
     CDS             11219..11989
                     /gene="yjjP"
                     /locus_tag="ACHKAU_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO5531807.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            11980..12453
                     /gene="yjjB"
                     /locus_tag="ACHKAU_002782"
     CDS             11980..12453
                     /gene="yjjB"
                     /locus_tag="ACHKAU_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO5531808.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            12560..13099
                     /gene="dnaT"
                     /locus_tag="ACHKAU_002783"
     CDS             12560..13099
                     /gene="dnaT"
                     /locus_tag="ACHKAU_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO5531809.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            13102..13839
                     /gene="dnaC"
                     /locus_tag="ACHKAU_002784"
     CDS             13102..13839
                     /gene="dnaC"
                     /locus_tag="ACHKAU_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO5531810.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            13888..14382
                     /gene="yjjA"
                     /locus_tag="ACHKAU_002785"
     CDS             13888..14382
                     /gene="yjjA"
                     /locus_tag="ACHKAU_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO5531811.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            14636..16927
                     /gene="opgB"
                     /locus_tag="ACHKAU_002786"
     CDS             14636..16927
                     /gene="opgB"
                     /locus_tag="ACHKAU_002786"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO5531812.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(17066..18088)
                     /gene="lgoD"
                     /locus_tag="ACHKAU_002787"
     CDS             complement(17066..18088)
                     /gene="lgoD"
                     /locus_tag="ACHKAU_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO5531813.1"
                     /translation="MSTMKVLICQEIKNLVWTVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNFHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            18227..19132
                     /locus_tag="ACHKAU_002788"
     CDS             18227..19132
                     /locus_tag="ACHKAU_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO5531814.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(19303..20955)
                     /locus_tag="ACHKAU_002789"
     CDS             complement(19303..20955)
                     /locus_tag="ACHKAU_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5531815.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNL
                     AALNARRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
     gene            21635..22606
                     /locus_tag="ACHKAU_002790"
     CDS             21635..22606
                     /locus_tag="ACHKAU_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO5531816.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            22670..23143
                     /locus_tag="ACHKAU_002791"
     CDS             22670..23143
                     /locus_tag="ACHKAU_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO5531817.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            23140..24411
                     /locus_tag="ACHKAU_002792"
     CDS             23140..24411
                     /locus_tag="ACHKAU_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO5531818.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(24454..26124)
                     /gene="tsr"
                     /locus_tag="ACHKAU_002793"
     CDS             complement(24454..26124)
                     /gene="tsr"
                     /locus_tag="ACHKAU_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO5531819.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAAAALEEQAS
                     RLTEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(26243..26689)
                     /gene="hpaR"
                     /locus_tag="ACHKAU_002794"
     CDS             complement(26243..26689)
                     /gene="hpaR"
                     /locus_tag="ACHKAU_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO5531820.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         6696 a         6934 c         6441 g         6687 t
ORIGIN      
        1 gcctacgaag ggtaaactac ccactaacgc taaacaacgc atacgtatta ttaagcaaca
       61 cttcggcaat ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca
      121 cagcctgttc cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt
      181 cggtttccag caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg
      241 cgcgtggata ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct
      301 cggcctgttg caggctgccg gaaaaaccgt gaaccacgcc agtgcacggt aaatcgtgac
      361 gtttaagatg catcgccagt ttgtcgtgtg tacgccggga atgaaggatc accggcagat
      421 cgtagcgttt cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaaattgcg
      481 gatcgtcgcc gaagagatcc agaccgatct cccccaccgc caccaccttc gccggacgcc
      541 tttccagcgc ctgctgtagc tgatcaagag acacatcgct atgtttttcc aacatccccg
      601 gatgcaaccc caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacgcggg
      661 caaaattttc cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc
      721 gttgcaggct ggcctcttca tcgccactaa acggcgggaa atcaaaatgg cagtgggtat
      781 cgataaaacg gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg
      841 agcatcgctc acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat
      901 cgattccggc gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag
      961 cttgccaacc gtggcgagga aataacgacc gcataaacgc ccaagtttat agtcttcacg
     1021 cagcgccgga atccgactgc caagcgcgat actatgtaat ggtttcggtg gataaatttc
     1081 gaatatccgc agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt
     1141 ttcatgatgc tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc
     1201 catgcgtttg aaccactgcg gcgtgtagta catttgtgac ggcacagtgc ggatgacgac
     1261 caacgtttta gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc
     1321 atccaggtag ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact
     1381 ggaggcgcga atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta
     1441 gtcatcctga cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc
     1501 ggtatccatt tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat
     1561 aagatttcct ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg
     1621 catgatgact ttgcgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc
     1681 cccggcagat gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc
     1741 cagcacgcca gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg
     1801 tccaggctgg aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg
     1861 ctgccccacc tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat
     1921 tattcgctaa aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat
     1981 ggtctgaaat ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc
     2041 cgacgacaaa gacaatttta gctgcgcctg cagcggtacc ggccagacca ccaaacccaa
     2101 gtgcggcggc gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct
     2161 cctttaccat agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt
     2221 tttagtgtgg tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt
     2281 acttagtttt cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt
     2341 cagcacggtc gctctgtgcc tgggaatcga cggtaccgga gagctgaacc acgccgtcgg
     2401 tggtttcaac tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga
     2461 tttcactggt ggtggcggtg tcacccgcgt agcccttcac agagccttct ttagcgtcgc
     2521 gaacgtgcag tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg
     2581 cctcttcggc ctgagcctgg ctttcaacga aaccgctcag ggtcacgact ttttgatcgg
     2641 ttttgacaga gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt
     2701 tcgcggtgat ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt
     2761 tttgccctgc gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg
     2821 cgacggcaga ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag
     2881 tcatcgattt attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt
     2941 gctgaatagg gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat
     3001 gggtgatgaa taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca
     3061 atcgacggct ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg
     3121 tcattacttt gttaaatata gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca
     3181 taagcaaaag tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga
     3241 cagggcgcag cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa
     3301 ctgaacgtcc ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta
     3361 agcgaggtta tcggcgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc
     3421 gaatttcttc gcgtctgcac attctaccca gcgggcggtg gcaacgctga ctgattcata
     3481 tactgcttca acgttgtatt cgctcttcag gcgcgatacc actacatcaa actgcagcac
     3541 accaactgca ccaacgatca gatcgttgtt ggagatcgga cggaacacct gcaccgcgcc
     3601 ctcttcggaa agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag
     3661 gcggatacga cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc
     3721 accctgggta aaggtgtcgc cgatctgaat ggtgccgtgg ttgtgcaggc cgaggatatc
     3781 gccaggatac gcttcttcaa cgtgcgaacg gtcacccgcc ataaaggtca gcgcgtcaga
     3841 gatcaccacg tctttcgcgg tgcgcacctg gcgcagcttc atgccttttt catatttacc
     3901 ggataccacg cgcataaacg ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat
     3961 tttaaatacg aagccggtaa atttgtcttc gctcgcttct acggtacggg tatcagtctg
     4021 acgcggcatc ggcgcaggag cccactccac caggccatcc agcatatgat cgacaccgaa
     4081 gttacccagc gcagtaccga agaataccgg agtgatttcg cccgcaagga acaattcttt
     4141 gtcgaactcg ttagacgcgc ctttcaccag ttccagttcg tcacgcagct gctgcgccag
     4201 atcttcacca accgcagcat cgagatccgg gttattcagc cctttaacaa tgcggacttc
     4261 ctgaatggtg tgacctttac cgctctgata gagataggtt tcgtctttat aaaggtggta
     4321 aacgccttta aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccgatttt
     4381 cagctcgttc tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt
     4441 gttcataaag gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt
     4501 acgatcttca acaccttttg cggcgtcgat aaccatcagg cagcagtcca ccgccgtcag
     4561 ggtacgatag gtatcttccg agaagtcttc gtgccccggg gtgtctagca ggttaaccag
     4621 gcaatcgtga tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc
     4681 catctccatc cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccggc
     4741 ggtctgaatg gcctgtccga acagcagcac cttctcggtg atggtagtct taccggcgtc
     4801 cgggtgagaa ataatggcaa aagtgcggcg cttagccacc tcttgcaaat aaggagacaa
     4861 cgtcataatt ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa
     4921 ttgcggctat tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc
     4981 cagttcgtgc aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatt
     5041 gtgcgcattc agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc
     5101 ggcagtgtcg ccaaccatca gcacgcgtga acggtcagga ttgcccgcct gttccagcgc
     5161 ataatcgaaa attttcttat tcggtttagc aacgccaact tcttcggaaa tcaccagcaa
     5221 atcgaagtaa tcacgcaggc ccgtgcgttc cagacgcact tgctgcaagg cgctaaagcc
     5281 gttggtgatg atgccgattt tggcgttgcc acgaatggcg ttaagcagag aaaccgcgcc
     5341 cggcagcggc gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc
     5401 ttcaactttc agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt
     5461 gatcgcgccg ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa
     5521 atcttcagcg gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga
     5581 gtcaaaggta aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc
     5641 accttgtctt acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg
     5701 gtggggtagt aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca
     5761 atggcggcag catttgaggc acggacttcc agccagagcg tcgccacgcc gcgtttttcc
     5821 agttcgtcga tcagatgttc cagcagcgcc cttcccaatc cctgacgctg atagtcagga
     5881 tcgaccgcaa tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc
     5941 gccattttgc cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca
     6001 aaggtttttt cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt
     6061 aaatcagtcg tttcgaggga agaaatcgtg ttcatatgtg caaatttgtt gccataacgc
     6121 ggcgcgtgcc gttgggtttg cccgtaaatc ggtgagcgcc ggtgatgcca cctgagcgcc
     6181 ttccagtgat agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg
     6241 cagcatcgcg attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc
     6301 gcgcagaaca tcgctcacta aaggatcagt cagggcggga agatcgtttg ccaccatcac
     6361 cagacggacg tgtgccggga tcgcaatggc aatttccccc tgcaacgcgc cagggcgacg
     6421 cagcgaccac tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc
     6481 gaatcgctcc tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata
     6541 tggctataat cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca
     6601 agtgaagtct tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga
     6661 gacttacagg atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag
     6721 caattccacc actggcaggt attaagccgc cagatggggg ataacgcccg ttttagtctg
     6781 gtcgccaagg cggatgacgt cgcagattgc gatacgctga tttactactg gccgaagaac
     6841 aaaccggaag cccagttcca gttgatgaat ttactttctc tgctgccggt ggggacggat
     6901 atttttgtcg ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat
     6961 tatgcgccgt tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg
     7021 gaaaaacagc cagtatttga tgcggataaa ttctggggcg aatacagcgt cgatggcctg
     7081 acggtcaaaa cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg
     7141 ctgctctcga cgttaacccc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg
     7201 ggtgtgcttt cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat
     7261 gtctctgccc cggcggtaga agccagccgt gcgacgcttg cggccaacgg tgttgaaggt
     7321 gaagtctttg ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc
     7381 aacccgccgt tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc
     7441 ggcgcggtgc gtcatcttaa taacggcggc gagctgcgaa ttgtagcgaa cgccttcctg
     7501 ccttacccgg acgtgctgga tgagacattt ggcttccatg aagttatcgc gcaaaccggg
     7561 cgcttcaagg tgtatcgcgc cattatgacc cgccaggcga agaaaggtta agtatcccgc
     7621 cgcaagcctt gtcggatgcg gcgtgctcgc cttatacaac ctacaaaacc tgcacgttag
     7681 cccttcgtag gccagataag acgcgcccgc gtcgcatctg gcatctgcac ccaaataccg
     7741 tgtcggatgc ggcgtgaacg ccttatccga cctacaaaac ctgcacgtta gtttcctgat
     7801 ggacattttt ccagcaatta cacctccggc gataattaac tattgacgaa aagctgaaaa
     7861 ccactagaat gcgcctccgt ggtagcaatt ctttttaaga attgatggta tgcgaaggtg
     7921 gcggaattgg tagacgcgct agcttcaggt gttagtgtcc ttacggacgt gggggttcaa
     7981 gtcccccccc tcgcaccaat tatctttact tcctttcttg tttcttcctt gatttttcat
     8041 ccctgattca aattcattag catcagcatg atgccagcaa taagcgccag gcacgatggt
     8101 agcaaaacgt aatgccgtaa ctgtttgtga tacagcatca ccggcgtcaa tatcaatccc
     8161 atcagaatga caatccgcca ttgatccggc gataaatccg catacatcag ccctgcaatc
     8221 agcaatcccg gcagcaacac tccccagcca ctgcccagcg tacgttgcaa catggtttca
     8281 tctctttcat tgataatgat aaccaatatc atatgataat ttttatcatt tgcaagccag
     8341 agaaatccct tgctatcggg taaacctatc gctaggatta gcaatcatta tcatttagat
     8401 tactatcccg attatggcct atcgttccgc accgctctat gaagatgtca tctggcgaac
     8461 gcatctccag ccgcaggatg ctgggcttgc acaagccgta cgggcgacga tcgccgaaca
     8521 tcgtgaacat ttgctggagt ttatccgtct ggatgaaccc gccccgcttc gcgccatgac
     8581 gctggcgcaa tggtcatcac cgaatgcgct aagttctcta ctggcggtct attccgatca
     8641 tatctatcgc aaccaaccaa cgatgatccg cgagaacaag ccgctgatct cactatgggc
     8701 gcaatggtat atcggcctga tggtgccacc attaatgctg gcgctgctga cgcaggaaaa
     8761 agcgttagat gtgtcgccgg aacatttcca cgttgagttt cacgaaaccg gccgtgccgc
     8821 ctgtttctgg gtcgatgtgt gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg
     8881 aatggaaacg ttaatcagcc aggcgctggt tccggttgtg caggcactag aagcgacggg
     8941 agagatcaac ggcaaactta tctggagtaa taccggttat ttgatcaact ggtatctgac
     9001 tgagatgaaa cagctgctcg gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt
     9061 tgagaaaacg cttactaccg gtgaagataa tccactctgg cgtaccgtgg tactgcgcga
     9121 cggcctgctg gtgcgccgca cttgctgcca gcgttatcgc ttaccggatg tgcagcaatg
     9181 tggcgattgt acgctgaaat gaaagcactg ccggggaagt aaacccggca tcatgcggat
     9241 taatagggat gcaacacatt acttgcttcg caatgccgca tacataacag aatatctgcg
     9301 gcctccaaca accctgcatc agaactgacg cccagtttcg acatcacatt aaatttatgt
     9361 gcacggatcg ttttgatatt acgtttaagc tgctcggcaa tttgtgtcat tgagtaacca
     9421 cgcgacataa agcgcaatat ttctctctcc gtcgggctta gcgtccggct ttggttaatg
     9481 taccactgat tgttcagtcg gtcggtcgcc tgacgtacac catttaatga caaaaagagt
     9541 tcctgatgaa aaatctccag cgttgacgct ttacttaata caccgtccag cggtgatggc
     9601 gacaatgaac caatcagccg agcttcaata tcatcatccg caataactaa acgccgggta
     9661 cgcggaagct taatcgccag ttcagtcagg caagataatc cctctctgcg ctcacttctc
     9721 atggcagaaa aagaaaaaat gaccgccgca aacgagatcc gcgtcatggc atcctgaaat
     9781 gactcctgac tggtataggt atggagctta taagggttac ccgcaaactt tctgaataaa
     9841 ctctcaatac cgatactact catgacgcat ttttctatga gtgcgacatt tctcttctta
     9901 attcggctgt gttccattct gcggcatctc cataaacaga aggcaagtta agttctcttg
     9961 caccctgcgc gctatttatc catgcataca tttcagcgtt gctgtgaatt gccagtcgac
    10021 gcatcgcact gtttttttgg gcgctgatag ttttattact tttcttaagc aatgaagcta
    10081 tctggttgat gccccatcct ttacctaata gccgcagtac cttgcgctct gaaagagtga
    10141 gaatgacgga gcgatagcta aaatcatgag tttcagtcat tgccggagag gtcagcatgg
    10201 cgctgatacg ctctgcgtgc gtattgccgg aacgtacggt cttgaccaga ttctcaatgg
    10261 gttcaacatc agacaataac gtcgccgtag ggcacatgag cagttctact gcctgggaat
    10321 accaggagcg tgacaccatg aaaacccagt gaatttcccg atattgtgag attaaagaat
    10381 aataatgttc acaaacagaa cgtggatctt cactttcacc ggctaaatca gcaatgacta
    10441 atccggaacg gcgtaattgt aataaggtaa ggtcctccgc agaggcgctg gatattattt
    10501 catattcagg gaagtgagtc ctcatgacct cttttaaccc tgcttgcata acaggtattt
    10561 tactgataac aattccattt ttgcagcatc ctggcaacat attccctcca catccactgt
    10621 ctaaaaatta tgatggttta ttcaggatat tcattcctta aatgtttcca gcaaattaat
    10681 agattaagcg aataaattat ccttagcatt cactttcata tcagcatact cctgctgcac
    10741 aacaactcca ctgcatgtgt cagaaggtta gcatcaaatg ataacctggt gaaatagcct
    10801 ggagacattt aaaatatcta cattaagttg ttaggaatac ttaatgaaaa acagaaatca
    10861 cattcattta atttataatt aaatgatatc ccttcctgca gaatgcaaaa tcacacaacc
    10921 aatatttatt caatgaaatc tgatggtttt aacttgttaa cattaaaaaa cgtgcccggt
    10981 tatctattat cctgattcgt aaaggccaaa caataaattc attatgatga taatattcat
    11041 gtgatagttt gtttgcggcg agagataatt cgctttttat caccgagctt ctcccgataa
    11101 ccaggattaa tcagcgcaat tttgatctta acctaccgca aaacaaccgt gcttcatgat
    11161 acaaatggat tctcattatc tgaacaatac gcagcacgtc tatgacaaag ggcgagttat
    11221 gcaaactgag caacagcgag ccgtaacacg gctttgtatc cagtgtggat tatttctttt
    11281 gcaacatggt gcggaaagcg cgttggttga tgagctttcc tcacgactgg gtcgggcgct
    11341 gggaatggac agcgtcgaaa gttctatctc ttcgaacgcc atagtgctga caaccattaa
    11401 agatgggcaa tgcctgacat cgacacgtaa aaatcacgat cgcggcatta atatgcatgt
    11461 ggtgactgaa gtccagcaca ttgtgattct tgcggagcat catctgctgg attacaaagg
    11521 cgtagagaaa cgatttagcc aaattcagcc attacgttac ccaagatggc tggtagcctt
    11581 aatggttggc ctttcttgcg cctgtttctg taaactcaat aacggtggct gggatggtgc
    11641 cgtcatcacc ttctttgcca gtacgaccgc gatgtatatc cgccagctgc tggcacaacg
    11701 tcatcttcat ccacagatca acttttgcct taccgctttc gccgccacca ccatttccgg
    11761 attgcttttg caactcccca ctttcagcga tacacccacc attgcgatgg ccgccagcgt
    11821 tctgctgctg gtgccgggct ttccgttgat taatgccgtc gccgatatgt ttaaaggcca
    11881 catcaatacc ggactggcac gctgggcgat cgccagtctg ctgacgctgg ctacctgcgt
    11941 cggcgtggtg atggcactga cgatttgggg gctacgcgga tgggtgtgat cgaatttctg
    12001 ttagcgttgg cgcaggatat gatcctcgcc gccattcctg cggtcggctt tgcgatggtg
    12061 ttcaacgttc ccgttcgggc gttacgctgg tgtgcgctgc ttggcgcgat tggtcatggt
    12121 tcacgaatga tcttaatgac cagcgggctg aatattgagt ggtcaacttt tatggcttct
    12181 atgctggtcg gcaccattgg tattcaatgg tcgcgctggt atctggcgca tccgaaagtg
    12241 tttaccgtgg cggccgttat ccctatgttc ccgggcatat cggcttatac cgcaatgatt
    12301 tcggcggtaa aaatcagcca gttaggttac agcgaaccgt tgatgattac cctgttaacc
    12361 aactttctta cagcttcatc gattgttggt gcgttatcca tcggtctttc cattcctgga
    12421 ttatggttgt accgcaagcg ccctcgcgta taaaattgcc tcgcttaagc acacggatga
    12481 gagacagcct cctctcctcc gtgtgttact ataaaagtta tctcccttct cgttcatcgt
    12541 tccatatttg agaaacagta tgtcttccag agttttgacc ccggacgtcg ttggtattga
    12601 cgccctggta cacgatcacc aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt
    12661 atttgctaac aatgccccgg cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct
    12721 ggcgctggaa gaaaagctgg cgcgtccggg aagcgatgtc gctctggacg atcaactcta
    12781 tcaggaaccg caagccgctc ccgttgctgt ccccatgggg aaattcgcca tgtatccgga
    12841 ctggcaaccc gatgccgact ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga
    12901 gccggtgacc accgaagaac tggcctcatt cattgcctac tggcaggcgg aaggtaaagt
    12961 ctttcaccat gtgcagtggc aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag
    13021 caacggcgga ctgccgaaac gagatgtgaa tacggtcagc gaacctgaca gccaaattcc
    13081 accaggattc agagggtaac gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa
    13141 atgatgcctg cccatatcaa acctgcattc aaaacgggtg aagagctact ggcctggcag
    13201 aaagaacaag gggcgatccg ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg
    13261 cagcgcacct ttaaccgctc cggtattcgt ccactgcatc agaactgctc ctttgagaac
    13321 tatcgcgttg agtgtgaagg gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa
    13381 gagtttgacg gcaacatcgc cagcttcatc ttttctggta agccaggaac cggcaaaaac
    13441 catctggcgg cggcaatctg caacgagctg ctgctacgcg gtaaatccgt attgatcatc
    13501 accgtggccg atattatgtc ggcgatgaaa gataccttca ggaatagcgg taccagcgaa
    13561 gaacaactgc ttaacgatct gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg
    13621 cagaccgaat cgaaatacga aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct
    13681 tccaaacgcc caaccgggat gctgaccaac agtaatatgg aagagatgac caagttgctg
    13741 ggcgaacgcg tgatggaccg tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg
    13801 gatagctacc gtagccgggt aacaggtaaa gagtattaat ccccgtttct ggcggtatac
    13861 tgcgacacaa ttccaccttg agtaatgatg atgaaaactg ttaaacatct tctgtgctgt
    13921 gccattgccg ccagcgcatt aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg
    13981 ctctccagcg ccgccagcga acttggcaac caaaacagca cgacacagga aggcggttgg
    14041 tcgctcgcgt cattaactaa cttgcttagc agcgggaacc aggccttaag cgcagataac
    14101 atgaacaacg ccgcaggcat tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc
    14161 gatgccgaaa acatcaagaa ccaggtgctg gaaaagctgg gcctgaacag tgaagaacaa
    14221 aaagaagaca ccaactatct ggatggcatt cagggtttgc tgaaaaccaa agatggtcag
    14281 caactcaatc tggataacat cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc
    14341 tgcgatctgg tgttaaagca ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt
    14401 ttagctgtta cccggctgac gcggctctct cctgcccttt tccctgctcc tggacggttt
    14461 tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa ctaattccta
    14521 acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca ttacattgtg
    14581 cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc gcccgttgtc
    14641 agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat ggaaagcggg
    14701 acgtaacacc tggtggtttg cagccacatt aacggtgctg gggctatttg tcgttttaaa
    14761 tatcaccctg tttgccagcg actattttac tggcgctggt attaacgacg cggttctcta
    14821 taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg
    14881 cattgtgctg gggctgacag cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg
    14941 ccatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc tgggttcggt
    15001 ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt cacgcgatgg
    15061 cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg acccgaaact
    15121 caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata acgaagcttt
    15181 cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac
    15241 gcagcagctg ccgggaacag attacacgat tgcgggcatg gtggcttctc agtgcggcat
    15301 tccgcttttt gccccatttg aaggtaacgc ctccgcttct gtttccagct tcttcccgca
    15361 gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg
    15421 cgcgaatctg cgttttgccg gtaaagatgt gttcctgaag tcgcatggct tcgaccactt
    15481 atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca acgactgggg
    15541 attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc
    15601 aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg atggttttat
    15661 ctcgcgtacc tgtaaccgca aaaaatatga ttttgacggt aagccgaacc agtcattcag
    15721 cgcggtaagt tgcagccagg agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc
    15781 gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga acaacacggc
    15841 gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc gtggcgacaa
    15901 gccgcagcaa gagacgctgg cagtgaagcg taacacgatg gataacggcg cgacggtgct
    15961 ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat ccgggcagtc
    16021 aatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc cggatatcat
    16081 ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc agaaaaacat
    16141 gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt cagacaaacg
    16201 cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc tggccgattt
    16261 cgctccacgc gacaatttcg tctgggttga ccgttgctac aagatggcac aactctgggc
    16321 tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg gcggtcagca
    16381 aattgttcag catgttgaca aaacaacatg gcagggcaaa acggcattta aagatacggt
    16441 catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg ataacgatat
    16501 tcgctacaaa gccgacagtt tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca
    16561 gtttagtggg atttcccgtc cagagtcgtg gggccgctgg tccaacgcgc agctgggcga
    16621 tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg tgattaccgc
    16681 caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca atgaagaaca
    16741 aacccttgtg ctgggcaatg aagtgaccac caccacactg catttcgata acccaaccga
    16801 tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag ggaatatcct
    16861 cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagttg tggaacgtga
    16921 agggtaattc ctgactgatg tgtaggtcgc ataaggtgcg acctacacac cttctccggc
    16981 ctacaaaaca tcattgagaa acagaaaaaa acagcgccca acacttcccc ggacgctgca
    17041 tggcaacttt aataatctcg ccttatcaga aagtaattac acctttaatt aactcacgat
    17101 tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag cgatgggtta
    17161 acatcatgtc agcagtgatt tttccttccg ccatcagtcg accgacttta gcaaaatctt
    17221 ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat tccggatcgg
    17281 agaactgcaa ctcaccttta aacaggccga caaataccac cgtgccgccg tgacgaatca
    17341 gattcacggt gttattcatc gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca
    17401 gcgaaccacc aaactgcgcc cgcaactgcg cgtcaaaatc ttcggctgac gggtccagca
    17461 cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta tccgccacca
    17521 ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc
    17581 cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa
    17641 tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg ggcaaaatgt
    17701 tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg ccaatgactg
    17761 aaattttttc acagcaattg gtacgcccgc ttttacacgc cgggcattgc tgacaagaaa
    17821 tatacggaat taccgcaact tgctgtccga tattaaaatt gtgaaagttt tttcctgatt
    17881 caataatctc cccacatatt tcatggccta aaacgcgtgg atagctaaaa aatggttgat
    17941 tgcccgccca ggcatgaata tctgtaccgc aaatcccgac ggttttaatt ttaattaatg
    18001 cttcattatc gcctggcacc ggtctttccc ggactgtcca tactaaattc tttatttcct
    18061 gacaaattaa tactttcatt gtggacatga ttcaccgctc cttttatttt atgactttca
    18121 gatatcgtcg aatttataag agcacctgaa tttacccata aaatatgcgg atgatcacgc
    18181 ataaaaatgt tttaaatcgg tttttaatgc cggactggag ggaagaatga gtcgttcgca
    18241 gaatttacgt cataacgtca taaatcaggt gattgaagat atggcgaggg gcaatattcc
    18301 ttctccgctg ccttcacaaa gtgggctggc agaaatgtat aacatcagtc gcactacggt
    18361 acgccacata ctccagcatt taagcgcatg tggcgtgctc accctggtgg ggaaaaatta
    18421 tgttatcgcc cgcaaacccg aacagaacga tggatttgat tgcatcacgg cctcgctgaa
    18481 agagcaaaac cgtcttttcg agcaggcatt tttcaccatg attaacctgc gccagttacg
    18541 cgcgggagaa agcttttccg aacttcagct ggcgcgagcg gcaggcgtca gtccggtagt
    18601 agtacgagaa tatcttttga aattcggtcg ttataatctt attcaaagcg aaaagcgcgg
    18661 ccagtggagc atgaagcagt tcgatcaatc ctacgccgag cagctgtttg agctacgcga
    18721 aatgctcgaa acacattccc tgcaacactt tctcaacctt cccgatgacg atccccgctg
    18781 gttgcaggcg aaaaccatgc tcgaacgtca caggacattg cgcgacagca ttggcagcaa
    18841 ctttcggatg ttttctcaat tagaccgtga ttttcatgcc atgctgttat ccgctgctga
    18901 caacatcttt tttaatcaat cacttgaaat tatctcggtg atctttcatt ttcactatca
    18961 atgggatgaa agcgatctca agcagcgcaa tatcattgct gttgatgagc atatgaccat
    19021 tctcagcgcc ctgatttgcc gtagcgattt agatgcaata acggcattgc gaaaccatct
    19081 ggataccgcc aaacaatcaa tgattcgttc catcaggcaa catcaccgat aaattaagta
    19141 cagattaaaa acattgattc ttggtttata tcactatgtt gtattaatgc agtattaatc
    19201 caggcgattg ttctgcccat aaggaaatac atagggaata aacgatgctg gaagtattac
    19261 tctcagccat catttaactc cctattactg gtagcgataa aattatcggc tatccatgat
    19321 taacgtttcc agcacatcta attcactgac tatttttgcc agtgtttcat cactgacttc
    19381 ttgccgtgca cgcatctggt aaatagccgc acgcctggca tttaacgctg ccagattaaa
    19441 tgttctttcc agcttcgcgc gctgcatatg taaagaatta tcattttccg acatatcatc
    19501 ttcgctacgc cgcatataac cgataactct ggcccctgtt tccataatca actcgttgtc
    19561 gaggttttct gtactggtgc gtagcaaacg ttgctcagtc ttttgcacac tgagtatggc
    19621 cattttggcc ataaacagat cggcagcttc ctcttctttt tgccggttat cgctgaaaat
    19681 gcccacattt ttccccagta gtaacggcag aataataatg ccgcaaacca gcgaaaagag
    19741 gatcacacct gtggcgagaa ataccagtaa gtaacgcccg ggaaaaggtg tgccatccgg
    19801 cagcaataac gggatagaaa gcacccccgc aagcgtgatg gctccgcgca cccccgcaaa
    19861 ggtcgccagc agcatatgac gcgttttata tccagcaaac agcatcggat gtttgccggg
    19921 tcgtttacgg ctatacagac gcatcagcca aagccagccc aggcgcacaa ccattaacgc
    19981 caggtagatc caaatgacat caaggagcag cgatccggtg tctaccagtg gatcgttttc
    20041 tgcacggatc agtgattccg aaataatgcc cggcagttgt aagcctaaaa tcagaaacac
    20101 catgccgtta aagacatatt caagcatcga ccagctactt ttagctttaa ttctcaggcg
    20161 gataggcgcg tggtggacta cgccaatgcg actggttgtt aaccccgcgg cgacagcggc
    20221 aagaatgcca gataagccaa aatgttccgc aaccagataa acaataaacg gcaacagaag
    20281 catcaataca atctgaatgg aaggttcatc gtgagcatat cgactgatca acagcagccc
    20341 tttgccatat aaccaggtaa cagcaatccc cacggcaata ccgcccaacg caacgacaaa
    20401 aaacgcaatg gttgcgccat gaacgctgaa ttccatcgtt cccatcgtca ctgcaacagc
    20461 aaatttcaac gccaccaggc cggaagcatc attcatcaac gcttcgcctt cgagcacttc
    20521 ctggatgttt ttgctgatac gccctttccc gacaatcccc agcaacgcca cggcatcggt
    20581 aggcgacaac acggcggcta atgcgaaagc agcgatgagc ggcacattcg gtaagagtaa
    20641 atgaatcaca tagccgacac caaaaatagt gattagcacc aacacaagcg ccaggctgat
    20701 aatttcccga ctgtgcttga tcaattcttt aggttgtatt tttgagcttt cagcaaacaa
    20761 caaggggggt ataaacaata agagaaaaag ttcaggattg aattccacat gcagaccaaa
    20821 tatgggctgg gccagtacag cgccagcggc aatctggatc aaaggaagtg gtatctgaac
    20881 aggagacaga cgcgtaagca cgctggttaa tgatactacc aacgtaagaa ttaaaattgt
    20941 taaaagtatt tccatataat aaacaggctt cctcattcat aaataatggg tattattaat
    21001 ttaacttcat aaaaagcgcc ctggttagca cttaacgtta atcattaatc acagcgttca
    21061 ctcacatgct caggaggaaa ccagtgagat gagttttcca gttgataaca aattcaccat
    21121 gtgaatattt actaccttta accgttgtaa cgaatcatcc gttactcttt gatctccttg
    21181 ccgaacaagt tatttagtga atgatggctt tcttttaacg ttagcatgaa ttatacgttc
    21241 agcaaatgta ttaaatcagc actaaataaa tttaatggat accaccattc tgcgtcaaca
    21301 cttagttttt atctgtaaaa atagccgcag ccaaattaat gcttttattt taataaatca
    21361 tcaaccgaat aagagatctt atcagcggta actaaactca tcagtgggtg ttccttgtaa
    21421 cgtcatacat tacataactt taagatatac aatcaaaaaa tgcattaaaa accgattaaa
    21481 agcagcaaag tattacccgg tgcacgaatt tatgacacat ttgcagccat tccgctattc
    21541 gctgactact tttcagtaat gactgctttt tcataaggtt tcactcccga caacaatgtt
    21601 ggagttttat ttccttagca tggagtaagt aaagatgagc aaaataatag cgatgctaat
    21661 tactgtcagt accctttttt taccgtcaat aatccaggca aaacctattt cgataaaagt
    21721 tgcctatgaa aataaccctg gagaacccct ggatgtggtc atgcgttact gggcagacct
    21781 attaaataaa aaaagtaatg gcgaaataac acttgcgctc tatccaagtt cccaattggg
    21841 ctcaaaacag gatgtcaccg agcaagccat gatgggcatg aatgtcataa ccttgagtga
    21901 tgtggccttt cttgccgatt atgaacccga tttgggtatt ttgtttggac cgtatctgac
    21961 tgacgatccg caaaaactgt tcaaaatata tgaaagcgac tggtttaaac aaaaaaatga
    22021 aagtctgaag aaaaagggaa ttcatgtggt aatgaataat tatctctatg gcactcgaca
    22081 gattatttcg aaaaaaccca ttcgcactgt tgacgatctc gcaggcttaa aaattcgtgt
    22141 tcctaataac gtcatgcaaa taaaagccat tcaggcgatg ggggcaacac caactcccat
    22201 gccattaggc gaagtttatc ccgcattaac ccagggcgtt attgacggcg tagaaaaccc
    22261 aatttcagtg ctgcaagggc aaaaactgta tgagcaggca agatatctga caatggttaa
    22321 ctacctgaca aatacctcag tatggatagg aggtgaagcc ttctttagca ccctttcacc
    22381 agaacaactg gagatgatcc accagaccgg ctatgaagcg ggtgtctata gccagaaact
    22441 cactcttgaa cgtgatgccg aaatgttgaa aaccatgcag gcagcgggcg tcgaagtgat
    22501 ctatcccgat actggaccat tccagaagaa agcacgcgaa gtttactctc aattcccgga
    22561 atggacacca ggtctgtacg aaaccattca acagcaactg caataagcat taatgctcag
    22621 cgtcctgcct catctctctt aggcaggaca cgcactaaca ggaggtttca tgcgtctttt
    22681 tggcaaagtg gcagaattta gtgctgcctt tgcgctattc gttctggttg tgatgacaat
    22741 tggagcggta tttatgcgtt atttcatcgg ccaaccgctt caatggacag aagaaatgtc
    22801 gggaatgctg atgatttggg tggtgatgtt aggtggagtg gtggcggaac gtgacagggc
    22861 gcacctgacc attcccttcc tgatggagat gcttccaggc aagctcaggc gtgttattgc
    22921 agtactggtc gccgttctct ccatcgcact gctgctgtat atggcctggc tgggctaccg
    22981 actagccgaa atggcgcagt ttaaagtgac acaacttctg aaggtttcct ggttctggat
    23041 cgatctggcg gtccctgtcg gtgcactggc aacggctatt tataccctct actggttaat
    23101 acacgatata aaacaatctg atagcggaga caaaatgcaa tgagttggtt attgattatt
    23161 ccgctaatga tcgtctgttt gtttctcaac attcgggtct atctggcgat gtttattggc
    23221 atcatcgctt actttttatt cttctccagc gtaccgattg aaattgccgt gcaaagactg
    23281 atcgcgccaa cgcaaagccc atcattgctg gcaattccgt tttttatctt gctcggcaca
    23341 ctaatgagct acaccggcat tgccgaacgc atcctgcaca ttgccaacgt tctggtgggg
    23401 aaaatgcgcg gcggacttgg cgtggcaaac attctcgtca gcaccatgat gggcggcgtc
    23461 agcgcctcta accttgctga cgcggcgatg ctgtcgcgca tgatggtgcc ggagatggag
    23521 cgtaaaggct acaaccgcgc gtttgccgcc gccattaccg ctggtggctc gttagtcacc
    23581 ccgatcatac cgccgggaat agccctgatt atttacggtc tggttgccga cgtctctatc
    23641 ggaaaaatgt ttatcgctgg gctggtacct ggtttgctat gcgcagtcgt tctgatgttt
    23701 accgtctacc tggtcgcccg caaaactaac gctaaaccct cgcgtgaaag ctggcctacg
    23761 ggcaaagagg tggtgttctc gctgggacag gcctggcctg cgctgttcct catttttgct
    23821 gtggtgggtg gtattcgcgc taacattttc accccaacag aagcaggtgc agcagcggtg
    23881 ctaatcgtgc tggcgattgg cttttttatc tatcgtgaaa tgcggatttc tcatgtcgtc
    23941 aaagcattgg gcgaaacggc acgcgcaacc gcatcggtga tgctggtaat catggccagc
    24001 gccgcgttgg gatggatttt ctcaatggaa catgctggtg tcgcagtggc gaattttgtg
    24061 acttcgctga ccgaaaataa atatatgttc ctgttaatta tcaacatatt gctgctgacg
    24121 ctggggatgt ttcttgaagg aaacgccatt ttgctgatcc tggtaccact attaaaaccg
    24181 gtggtcgaac actttggcat cgatccggtg cattttggca tcatcatgat cttcaacctg
    24241 tcgattggcg cattcacgcc accggtaggt accgtgatgc tgctggtatg taacatcacg
    24301 caagtttcgg tgggaaattt ctttaaacaa tccctgcctc tactggcggc tctactatta
    24361 atgctaacgc tggtgacata tatcccggcg atttcactgt tcctggttta ataataaaaa
    24421 cgccggatga aatactcatc cggcgtcatt gcgttaaaac gtttcccagt tctccccgct
    24481 atctgccacg gccattttac gcggcgcagc tggcgtcacg gtttttacca cagccgatgt
    24541 ttcacgctgc tgttgctgct gttgctgaat ccggaacact gccacagctt cggtcaggcg
    24601 actggcctgc tcttccagcg cggcggcggc ggcggcggcg gcagattctt ccaccagcgc
    24661 ggcgttctgt tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc
    24721 acggctctgc tcatcagaag cagaagcaat ttcgcccata atgtccgtca cgcgggtcac
    24781 agcactgaca atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc
    24841 cacttttccc accgagtctt caatcaggct tttaatttca cgagccgcct gagcgctacg
    24901 ctgggccaga ttacgcactt ctcccgcgac caccgcaaaa ccgcgtcctt gctcacccgc
    24961 acgcgcagcc tcaaccgccg cgttcaaagc cagaatattg gtctggaagg caatgccgtc
    25021 aattacgctg ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg
    25081 caccacgtta tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag
    25141 atggctggcc tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat
    25201 gctggctgcc gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcgtt
    25261 attgccggta gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc
    25321 accgacggta cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat
    25381 ctcattagag ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat
    25441 gctgtcaatc aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc
    25501 gaagatgacc gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta
    25561 ggaggcattg ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc
    25621 cacatactgc ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt
    25681 gattttgcct gcacctaata gttggatcag ctccgccagc gcattgtgat aaatatcgta
    25741 attacgtttg atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc
    25801 gtaatccgcc cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc
    25861 agcaacggtt gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg
    25921 gttgagggtg ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tggattgctg
    25981 ctggcgaatg gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaagaa
    26041 cagaccgcct gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt
    26101 cacaattttg atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg
    26161 gcctggaaag caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg
    26221 ctggttatca gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat
    26281 tacccagagc aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa
    26341 actgagcttc aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg
    26401 cctgcccctc tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc
    26461 gcaacactaa accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa
    26521 tgcaggcgcg atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca
    26581 cgatgcgcca ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca
    26641 tcgccgcttc tcgcgcctgg agcaacgcaa tggttagaga gtcgtgcatt atctttcccc
    26701 tgaaaaattc aaaatcatta atatagaaac agttaatcat cacgttaccg ggactgcg
//
LOCUS       DAZFJF010000063        26609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFJF010000063 DAZFJF010000000
VERSION     DAZFJF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.063"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1373)
                     /gene="djlB"
                     /locus_tag="ACHKAU_002795"
     CDS             complement(<1..1373)
                     /gene="djlB"
                     /locus_tag="ACHKAU_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO5531821.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKII"
     gene            complement(1370..2077)
                     /gene="ybeR"
                     /locus_tag="ACHKAU_002796"
     CDS             complement(1370..2077)
                     /gene="ybeR"
                     /locus_tag="ACHKAU_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5531822.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSVNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2241..3218
                     /gene="ybeQ"
                     /locus_tag="ACHKAU_002797"
     CDS             2241..3218
                     /gene="ybeQ"
                     /locus_tag="ACHKAU_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO5531823.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3288..3770)
                     /gene="ybeL"
                     /locus_tag="ACHKAU_002798"
     CDS             complement(3288..3770)
                     /gene="ybeL"
                     /locus_tag="ACHKAU_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO5531824.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4005..6587
                     /gene="leuS"
                     /locus_tag="ACHKAU_002799"
     CDS             4005..6587
                     /gene="leuS"
                     /locus_tag="ACHKAU_002799"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO5531825.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6602..7183
                     /gene="lptE"
                     /locus_tag="ACHKAU_002800"
     CDS             6602..7183
                     /gene="lptE"
                     /locus_tag="ACHKAU_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO5531826.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7183..8214
                     /gene="holA"
                     /locus_tag="ACHKAU_002801"
     CDS             7183..8214
                     /gene="holA"
                     /locus_tag="ACHKAU_002801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO5531827.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8216..8857
                     /gene="nadD"
                     /locus_tag="ACHKAU_002802"
     CDS             8216..8857
                     /gene="nadD"
                     /locus_tag="ACHKAU_002802"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO5531828.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            8881..9492
                     /gene="cobC"
                     /locus_tag="ACHKAU_002803"
     CDS             8881..9492
                     /gene="cobC"
                     /locus_tag="ACHKAU_002803"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO5531829.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPPPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9752..10069
                     /gene="rsfS"
                     /locus_tag="ACHKAU_002804"
     CDS             9752..10069
                     /gene="rsfS"
                     /locus_tag="ACHKAU_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO5531830.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10073..10540
                     /gene="rlmH"
                     /locus_tag="ACHKAU_002805"
     CDS             10073..10540
                     /gene="rlmH"
                     /locus_tag="ACHKAU_002805"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO5531831.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10571..12472
                     /gene="mrdA"
                     /locus_tag="ACHKAU_002806"
     CDS             10571..12472
                     /gene="mrdA"
                     /locus_tag="ACHKAU_002806"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO5531832.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12475..13587
                     /gene="mrdB"
                     /locus_tag="ACHKAU_002807"
                     /gene_synonym="rodA"
     CDS             12475..13587
                     /gene="mrdB"
                     /locus_tag="ACHKAU_002807"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO5531833.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13598..14686
                     /gene="rlpA"
                     /locus_tag="ACHKAU_002808"
     CDS             13598..14686
                     /gene="rlpA"
                     /locus_tag="ACHKAU_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO5531834.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14826..16037
                     /gene="dacA"
                     /locus_tag="ACHKAU_002809"
     CDS             14826..16037
                     /gene="dacA"
                     /locus_tag="ACHKAU_002809"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO5531835.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16148..16411
                     /gene="ybeD"
                     /locus_tag="ACHKAU_002810"
     CDS             16148..16411
                     /gene="ybeD"
                     /locus_tag="ACHKAU_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO5531836.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16512..17153
                     /gene="lipB"
                     /locus_tag="ACHKAU_002811"
     CDS             16512..17153
                     /gene="lipB"
                     /locus_tag="ACHKAU_002811"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO5531837.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17412..18365
                     /gene="ybeF"
                     /locus_tag="ACHKAU_002812"
     CDS             17412..18365
                     /gene="ybeF"
                     /locus_tag="ACHKAU_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO5531838.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            18574..19539
                     /gene="lipA"
                     /locus_tag="ACHKAU_002813"
     CDS             18574..19539
                     /gene="lipA"
                     /locus_tag="ACHKAU_002813"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO5531839.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(19640..19843)
                     /gene="tatE"
                     /locus_tag="ACHKAU_002814"
     CDS             complement(19640..19843)
                     /gene="tatE"
                     /locus_tag="ACHKAU_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO5531840.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(19972..20760)
                     /locus_tag="ACHKAU_002815"
     CDS             complement(19972..20760)
                     /locus_tag="ACHKAU_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO5531841.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            20853..21236
                     /gene="crcB"
                     /locus_tag="ACHKAU_002816"
     CDS             20853..21236
                     /gene="crcB"
                     /locus_tag="ACHKAU_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO5531842.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21291..21500)
                     /gene="cspE"
                     /locus_tag="ACHKAU_002817"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21291..21500)
                     /gene="cspE"
                     /locus_tag="ACHKAU_002817"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5531843.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(21675..22235)
                     /gene="pagP"
                     /locus_tag="ACHKAU_002818"
     CDS             complement(21675..22235)
                     /gene="pagP"
                     /locus_tag="ACHKAU_002818"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO5531844.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            22824..24209
                     /gene="dcuC"
                     /locus_tag="ACHKAU_002819"
     CDS             22824..24209
                     /gene="dcuC"
                     /locus_tag="ACHKAU_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5531845.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24250..24930)
                     /gene="dpiA"
                     /locus_tag="ACHKAU_002820"
                     /gene_synonym="citB"
     CDS             complement(24250..24930)
                     /gene="dpiA"
                     /locus_tag="ACHKAU_002820"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO5531846.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(24899..26557)
                     /gene="dpiB"
                     /locus_tag="ACHKAU_002821"
     CDS             complement(24899..26557)
                     /gene="dpiB"
                     /locus_tag="ACHKAU_002821"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO5531847.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         6727 a         6703 c         6527 g         6652 t
ORIGIN      
        1 aaaataattt taatgatgcc cagacagcct ggcccaccat cgtcaacggg cattggctgc
       61 ggttgcggct ccggttcaac agtttgcttc tcggtaaaat tcgccgcctg aattaataat
      121 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
      181 ggcattaatg attcttcggt tggttggttc gcctgcacta attgtttggc aactaaatat
      241 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
      301 tgcaaaacca tctcgaccag tgaacgcccc agagcagaga gtttttcatc gctgagtatt
      361 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga tataaagtag cggttttccc
      421 tgatcgttaa ccagttcacg agcttcaatc atcatcacca ctaacgggta ataatcgacc
      481 cgatgctgac ggcaccattg caatattaaa gcatccgcct gggtggaggg atactcgcgc
      541 cagtagtcac aaagttctgc cagcaagctt tcgccttctc cacaatagac gcgttgtgcg
      601 tattcataat aacgcgcatc ttcattatca gattcagctt cccgccacga ccgggcgtaa
      661 tccaggagtt cgggaattcc caggcgacct gcgctatacc agcggagtaa atttctgtgc
      721 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
      781 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
      841 acggcaacgg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
      901 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ttgcaaccac
      961 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
     1021 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
     1081 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgca
     1141 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
     1201 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
     1261 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
     1321 atatcgacgt cagtcgtttc ctctatttcg agaatcttcc agcaattttt cataggtcgc
     1381 tcatatcctt aagcgtttcc gggcatgcag aatagtaagc caatggttgc caggacaagg
     1441 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg acggaatcgt
     1501 cgtattcttt acgcgctaag aattcgcgta ataactgcag atgatcatct tcgggagact
     1561 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta cgataataac
     1621 gtagagcccg tagatgaatg cgcgactgga tccatagact ttcttcttcg ctcaaccagt
     1681 tattaagtac gcccattcgg cttaaaaaac ccatccggac ataatcccag gctttaattc
     1741 ctccctcacc acagcattcg gcggtgctgg cgacaaattg cgcataagcc tgaccttgct
     1801 catttagttc agcaagatag tcacgccatt cacatggcga ataacgaaac cagcggtgat
     1861 aaaaccctgc caaccgagcg gcatgaccgt tatcgatcat tcggtgaatt gtgcgcagca
     1921 ggtcatcacg ggcatcaatt ccccaggagg aagaaaggtc aaaaaagcat gtacctaaat
     1981 acattttggg tgatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
     2041 aaagtgcaaa caatattttt tgcgactcca tgtccatata atttatcctt aaatattcat
     2101 taaatgaata ttagccgcat tgcgaggctc gtttatttat acttttctga cgcgacataa
     2161 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tgttttatct
     2221 gcaaccataa ggaaatgata atgattttca cgtcaagttg ctgcgataat ttatcaatag
     2281 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
     2341 attataatcg cgatagcgca attgattctc cggacgacga aaaagccttt tactggctga
     2401 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
     2461 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
     2521 tacaaggcca tactttcgct tccaacgccc ttggctggac actggatcgt ggagaagccc
     2581 ccaattataa agaagcggtt gtctggtatc agatagccgc ggagagcgga atgtcttatg
     2641 cgcaaaataa tcttgggtgg atgtacagaa atggcaacgg agtcgcaaaa gactatgcgc
     2701 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagtgacgcg caaaacaatc
     2761 tggccgatct ttatgaagac ggaaaaggcg ttgctcaaaa caagacactc gccgcattct
     2821 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
     2881 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
     2941 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
     3001 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
     3061 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
     3121 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
     3181 tcagtcagga aattcgtgag actgaagatt tactgtagag ataaaaacaa gggccgggga
     3241 atttcattcg gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
     3301 gggcgtctct ggaactggtc atgaccacat ttcgggcata gcgtcagcac ttccggtgtg
     3361 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
     3421 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa cttcgcgcca ttcaagctgc
     3481 gttttatcgg tgatatctgc cagctcctgc cataagcttt ctttaatcac ccgcataaag
     3541 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
     3601 cgtcttacag ctcgcgtcag ctcatcgacc tcggttcgcg ttaactcccc tgtttttatt
     3661 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
     3721 tcgctcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
     3781 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
     3841 ccacaaactc tgtaaattaa tgtatgaata atccgggcat catttcaacg tcgagtgcgc
     3901 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
     3961 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
     4021 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
     4081 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
     4141 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgctacc
     4201 agcgtatgct gggcaaaaac gtcctgcagc cgatcggctg ggacgcgttt ggtctgcctg
     4261 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
     4321 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
     4381 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
     4441 aaggcctggt atataagaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
     4501 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
     4561 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
     4621 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
     4681 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
     4741 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
     4801 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
     4861 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
     4921 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
     4981 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
     5041 acgagtttgc ctctaaatac ggcctgaaca tcaaaccagt tatcctggct gctgacggct
     5101 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
     5161 aattcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
     5221 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgtcagc
     5281 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccgaccc
     5341 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
     5401 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
     5461 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
     5521 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
     5581 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
     5641 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
     5701 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
     5761 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
     5821 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
     5881 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
     5941 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
     6001 ctaaccgctt cctgaaacgt gtctggaaac tggtttacga gcacacagca aaaggtgatg
     6061 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
     6121 ataaaacgat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
     6181 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accaaccgat ggcgagcagg
     6241 atcgcgctct gatgcaggaa gcgctgctgg ccgttgtccg tatgcttaac ccgttcaccc
     6301 cgcacatctg cttcacgctg tggcaggaac tgaaaggcga aggcgatatc gacaacgcgc
     6361 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
     6421 ttaacggtaa agtccgtgcc aaaatcaccg ttccggtgga cgcaacggaa gaacaggttc
     6481 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt accgtgcgta
     6541 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
     6601 cgtgcgatat ctggcaacat tgttgttatc tctggctgtg ttaatcaccg ccgggtgtgg
     6661 ctggcatctg cgtgatacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
     6721 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
     6781 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
     6841 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
     6901 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagtgc
     6961 caaagtcttc cgttcgttct ttgataaccc gcaaatggcg ttagcgaaag ataacgaaca
     7021 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
     7081 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
     7141 tccggcaacg cctgcacgcg tctccaccac gctgggtaac tgatgattcg gttgtacccg
     7201 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
     7261 gatcctctgt tattgcagga aagccaggac gctgttcgtc aggtagctgc ggcacaagga
     7321 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
     7381 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
     7441 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
     7501 gacctgctgt tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
     7561 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
     7621 cttccccgct gggttgctgc gcgcgcaaaa cagctcaact tagaactgga tgacgctgca
     7681 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
     7741 cgtttatcgc tgctctggcc agacggcaaa ttgacattac cgcgcgttga acaggcggtg
     7801 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
     7861 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgaacc ggttattttg
     7921 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
     7981 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
     8041 ggcgaggcgt taaatcgctt aagtcagacg cagttacgtc aggccgtgca actcctgaca
     8101 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
     8161 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
     8221 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atcttaaacc
     8281 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
     8341 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
     8401 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
     8461 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caagggccgg acgtgccgct
     8521 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
     8581 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
     8641 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
     8701 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
     8761 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
     8821 actgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
     8881 atgcgactgt ggttaattcg tcatggtgaa acgcaagcga atatcgatgg tctttacagc
     8941 ggtcatgcgc ccccccccct gaccgcgcgc ggtattgagc aagcgcaaaa tctgcataca
     9001 ctgctagatg atgtttcctt tgacctggtt ttatgcagtg aactggaacg ggcacagcat
     9061 accgcgcgac gggttctcag tgaccgccag ctccccgtgc aaatcatccc tgaactcaac
     9121 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
     9181 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
     9241 tttcaggcat tttcgcaacg tgtggaaaga tttatcgcaa ggcttagtga atatcagcac
     9301 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgtcta
     9361 attggcatgc ctgccgaatc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
     9421 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
     9481 gagaatgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
     9541 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
     9601 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
     9661 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
     9721 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
     9781 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcagggcaa
     9841 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
     9901 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
     9961 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    10021 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    10081 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    10141 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggaaaacg
    10201 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    10261 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    10321 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtcagtc tactgattgg
    10381 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggcg gagcagagct ggtcgctgtc
    10441 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    10501 gtggagcatc accaccaacc atccttatca ccgtgagtga taaaggagct ttgagtagaa
    10561 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    10621 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    10681 caacctgtat aatctgcaaa ttgttcgctt taccgattac cagacccgct ctaatgaaaa
    10741 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    10801 ccctctggca ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    10861 cgtgcagcaa acgctggacg ctttgcgcag cgtggttgat ctgaccgatg acgatattgc
    10921 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattcccg tgaaaactaa
    10981 cctgaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    11041 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    11101 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    11161 caactatgcg gcaacgcatg atatcggtaa gctgggcatt gagcgttact atgaagacgt
    11221 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    11281 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    11341 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg tagtcaccga
    11401 tccgcgtaca ggtggggtgc tggcactggt ttccacgcct agttatgacc caaacctgtt
    11461 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    11521 ggtgaaccgc gccacacagg gggtttatcc tcctgcgtct acagttaaac cctatgtggc
    11581 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    11641 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    11701 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    11761 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    11821 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    11881 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    11941 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    12001 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    12061 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    12121 cggtatgtac ggtgttgcta accgccctaa cggtacggcg cataaatact ttgccagcgc
    12181 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    12241 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    12301 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    12361 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    12421 taccgatctg cctgcggaaa atcctgcggt tgccgcagcg gaggaccatt aatcatgacg
    12481 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    12541 atcttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    12601 attggcatga tggagcgtaa aataggccaa atcgcgatgg gtctggtcat catggtggtg
    12661 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    12721 attattttgc tggtggcggt agatgctttc ggtgccatct ctaaaggcgc tcaacgctgg
    12781 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    12841 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa cactggcatc
    12901 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    12961 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    13021 ctgattggcg tcgcagtagt gctggtggcg gcgttcattc ctattctgtg gttcttcctg
    13081 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    13141 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    13201 aaaggctggc tgcatggcac tcagtcacaa cttgaatttc tacccgaacg ccataccgac
    13261 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    13321 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    13381 ggtcgcgtca tggctggcgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    13441 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    13501 ggatcagcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    13561 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    13621 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    13681 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    13741 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    13801 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    13861 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacac agttgacggc
    13921 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    13981 aatgatcgtg gtgcgcatta atgatcgcgg cccctacggc aacgaccgcg taatttcgct
    14041 ttctcgcgca gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    14101 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    14161 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gggcaagttc
    14221 agtgtctggc ccgcagggtg acattcttcc ggtcagtaac tcgacgctaa aaagcgaaga
    14281 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    14341 tggtgtactg gaaagcagcg aaccgacacc tgcaccacag cccgttgtta cagctccgtc
    14401 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    14461 cggcaacttt atggtgcaag tcggggccgt tagcgatcag gctcgtgcgc aacagtacca
    14521 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    14581 gcgtatccag cttggcccat tcgccagtaa agccgaagcc agtaccttgc agcaacgttt
    14641 gcaaaccgaa gcccaattac agtcatttat taccaccgcg cagtagcgta aagcaggcat
    14701 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    14761 cggtagtgca tttgctatag tagggcactt tttttaattc catcacggat gtcgtagttc
    14821 agaccatgaa taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc
    14881 tttgcacagc ctttatctct gctgcacatg ccgatgacct gaatatcaaa actatgatcc
    14941 cgggtgtacc gcagatcgat gcggagtcct acatcctgat tgactataac tccggcaaag
    15001 tgctcgccga acagaacgca gatgtccgcc gcgatcctgc tagcctgacc aaaatgatga
    15061 ccagttacgt tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca
    15121 ctatcggcaa cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt
    15181 tcctcaaacc gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat
    15241 cgggtaacga tgcttgcgtc gccatggccg attttgccgc tggtagccag gacgcttttg
    15301 ttggcttgat gaacagctac gttaacgccc tgggcctgaa aaacacccac ttccagacgg
    15361 tacatggtct ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc
    15421 aggcattgat ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt
    15481 ttaacggtat tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg
    15541 acggcatcaa aaccggacac actgacaaag caggttacaa ccttgttgct tctgcgactg
    15601 aaggccagat gcgcttgatt tctgcggtga tgggcggacg tacttttaaa ggccgtgaag
    15661 ccgaaagtaa aaaactgcta acctggggct tccgtttctt cgaaaccgtt aacccactga
    15721 aagtaggtaa agagttcgcc tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt
    15781 taggggttga taaagacgtg tacctgacca ttccacgtgg tcgcatgaaa gatctgaaag
    15841 ccagctatgt gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg
    15901 gaactatcaa cttccagctt gatggcaaaa cgatcgagca acgcccgctg gttgtgttgc
    15961 aagaaatccc ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc
    16021 atcactggtt tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc catatactaa
    16081 gcatcagtaa aaaaactccc gccttctggc gggagttgct atttaattac gttacgccgg
    16141 agctgacatg aaaaccaaac ttaacgaact gcttgaattc cctactcctt ttacttacaa
    16201 agttatgggg caggcgttac ctgagctggt tgatcaggtg gttgaagtgg tacagcgcca
    16261 tgcgccaggt gactacaccc caacggtaaa accaagcagc aaaggcaact accactcggt
    16321 atctatcact atcaacgcca ctcatatcga gcaggttgaa acactgtatg aagaactggg
    16381 caaaatcgat attgtccgca tggttctgta actcgcttct ccgttacccg gctcctggtc
    16441 gggtaactcc cctctcctcg ctgtgatata cttttccccc acttttactc attctccacg
    16501 gagatgccgt tttgtatcag gataaaattc ttgtccgcca gctcggtctt cagccttacg
    16561 agccaatctc ccaggctatg catgaattca ccgatacccg cgatgatagt acccttgatg
    16621 aaatctggct ggtcgagcac tatccggtat tcacccaagg tcaggcagga aaagcggagc
    16681 acattttaat gccgggtgat attccggtga tccagagcga tcgcggtggg caggtgactt
    16741 atcacgggcc gggacaacag gtgatgtatg tgttgcttaa cctgaaacgc cgtaaactcg
    16801 gtgtgcgtga actggtgacc ttgcttgagc aaacagtggt gaataccctg gctgaactgg
    16861 gtatagaagc gcatcctcgg gctgacgcgc ctggtgtcta tgtcggggaa aagaaaattt
    16921 gctcactggg tttacgaatt cgacgcggtt gttcattcca cggtctggca ttaaacgtca
    16981 atatggatct ttcaccattt ttacgtatta atccttgtgg gtatgccgga atggaaatgg
    17041 ctaaaatatc acaatggaaa cccgaagcga cgactaataa tattgctcca cgtttactgg
    17101 aaaatatttt agcgctacta aacaatccgg acttcgaata tattaccgct taattccata
    17161 catcaatggc ccaatttaca ctgggtcatt actcataata cctgctaatg ctttacatct
    17221 ccttcgcgtt tgtatatttt ctttatcact tgcagaaatg cggtgccacg ccttgctccg
    17281 caactcaccc cattgattaa cctattgaaa tagttgatta tggtgagcaa aagcgttccg
    17341 ttccaattag acataagatt attgtaattt tttaaatatt atttttccat gaataaaatt
    17401 ggagtaagtg cgtggatagt aataatcaaa ttgaaccctg tttaagcaga aagtcgtcag
    17461 aaggtaaacc acaaatattt acaaccttac gaaatattga tcttaacctt ctgactattt
    17521 ttgaagctgt atatgtacat aaagggatcg ttaatgcagc gaaagtgctt aatctgaccc
    17581 cctcggcaat cagtcagtct attcagaaac tgcgcgttat attccctgac ccattgttta
    17641 ttcgcaaagg ccagggtgtc actcctaccg catttgcgat gcatctacat gagtatatca
    17701 gtcagggcct tgagtccatt cttggcgcgc tggatatcga aggaagctat gataagcaac
    17761 gaacgataac tattgcgacc actccctcgg tcggagccct ggtccttcct gtcatctatc
    17821 gggcgattaa aactcactat ccgcagcttt tactgcgcaa cccgcccatc agcgacgcgg
    17881 aaaaccaact cagtcagttt caaaccgatc tcatcatcga taacatgttt tgcaccaatc
    17941 gtacggtgca acatcatgtt ctgttcaccg acaatatggt gttaatttgc cgcgagggaa
    18001 atccactact ctctttagaa gatgacagag agactatcga caacgctgcg catgtacttc
    18061 tgttaccgga agagcaaaat ttcagcggtc tgcggcagag agttcaagaa atgtttccgg
    18121 accggcaaat caatttcacc agctacaaca ttttgacaat cgctgcactg gttgccaaca
    18181 gtgacatgtt agcgattatt cccagccgtt tttataacct gtttagccgc tgctggccgc
    18241 tggaaaaact gccttttccg tccttaaatg aggagcaaat agatttctct atccactaca
    18301 acaaattcag cctgcgtgat cccatcctgc acggagtaat agatgttatc cgtaatgcat
    18361 tttgaaaaag tattgttaca gccagatcac agcgcagaac gataaaatgg cacaatccaa
    18421 caacaatttt acattttcgc gaccgctttg gctgctttca ggtccgtttc aatgatatac
    18481 tgccagtcgt taattcaaaa atagttgata attacaacaa tctattgaat tgaaacgctt
    18541 tccttcgtaa ttcgcaactg gaacacgcac gctatgagta aacccattgt gatggaacgc
    18601 ggtgttaaat accgcgatgc cgataagatg gcccttatcc cggttaaaaa cgtggcaaca
    18661 gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc agcggactct
    18721 acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag
    18781 gaagcctcct gccctaacct ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc
    18841 ctcggcgcta tttgtacccg ccgttgtccg ttctgtgatg ttgcccacgg tcgcccggta
    18901 gctcctgatg ccaatgaacc agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt
    18961 tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc ccagcacttt
    19021 gcggattgca ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg
    19081 ccggatttcc gtggtcgtat ggatcgtgct ctggatattc tgactgcaac gccaccagat
    19141 gtgttcaacc ataacctgga aaacgtaccg cgtatttacc gtcaggtacg gcctggtgca
    19201 gattacaact ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg
    19261 accaagtctg gtctgatggt gggactgggt gaaaccaatg aagaaattat tgaggtaatg
    19321 cgcgacctgc gccgtcatgg tgtgacgatg ttaacgctgg ggcaatattt gcagccaagc
    19381 cgccatcacc tgccggttca acgttacgtt agcccggatg agttcgacga aatgaaagcc
    19441 gaagcgctgg cgatgggctt tacccatgct gcatgcggtc cgtttgtccg ctcttcttac
    19501 cacgccgatt tgcaggcgaa agggatggaa gttaagtaat cctgtaataa ttacttacat
    19561 ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg
    19621 gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt
    19681 ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg
    19741 ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca
    19801 gcgccgcaac tactagcagt ttggtaatac taatctcacc catagatacc ttcttgacat
    19861 ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat tcgacgcgcg
    19921 cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa
    19981 ttgcggcggc gcaaagcgac ggttgtttaa gacgggcagt tgcgcgcgca cctgacgcac
    20041 acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt
    20101 gacgccaaag ggatctataa tccggctttg accgatattt ttgttcccgc actcccccgc
    20161 cgccaccata taacaggtgg tatccagcgc acgagcggca agcaacgttg accaatgatg
    20221 ctctttgagc ggcccgcgaa cccaggcggc aggaagtacc aggatttcag ctccctgtaa
    20281 tgcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt
    20341 catcccctcc acctcgagta acggagcgat ttcattacca gcatcaacac ggcgtgattc
    20401 ctgaatggca aatgcatcat agagatgcaa tttggcataa cgggcgacga tgtttcctgc
    20461 ctgtagcgcc accagcatat tccatgcgcg ccccggcgtt gaaggaacat gaatcgtcag
    20521 aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cgtccgagga attcgccttc
    20581 cagcagctgt gctgatttaa ccgatagatc tgcatcatga tcatcgcgcg ccagcaatgc
    20641 ttccggcagg acgaacagcg atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca
    20701 aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcgg caaccagcat
    20761 tgccacctcc tgtcagagtc gtaaaattcg ccaacgacga ttattcggcg ttacacttat
    20821 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
    20881 ttggcggtgg tacgggaagc gtggcgagat ggctgttaag tatgcgattt aacccgctgc
    20941 atcaggcgat tccgttgggg acgctggcag caaacctgat tggggcattc atcataggaa
    21001 tgggattcgc ctggttcagc aggatgacga acattgatcc agtgtggaaa gtattaatca
    21061 ccaccggatt ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt
    21121 tgttacaaga gggccgcttt ggctgggcgt tactgaacgt tttcgtcaac cttctggggt
    21181 cttttgccat gacagcactg gctttctggc tgttttcggc ctcaaccgca cactaaagac
    21241 caaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc ttacagagcg
    21301 attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc tacgcgctga
    21361 ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac gaacacgtct
    21421 ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa ccacttaacg
    21481 ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac aaatgctgtg
    21541 tcggttacca gtacaccaat tgtggtacgc tttgtccagt gcaactttac taaaaagtga
    21601 taaaagtcgc gttatttttg atgttactta ttttagctat tgattttaaa gaagttacta
    21661 aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt gccattgttg
    21721 taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac ggaggccaat
    21781 ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac gccagcggtg
    21841 aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact ttcccatccg
    21901 tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc atacaggcca
    21961 tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca cggtcgctcg
    22021 ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt gatggcagga
    22081 atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc tctaaacgtt
    22141 gtcatccact catctgcgtt agcaaaaact ttaccaacgc tgattaactg aataaaaaca
    22201 aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac atttatcaaa
    22261 aatctactac tagcatagca aagctacaat taacataacc ttaatagacc caacataaag
    22321 aataatctga atagctgctc tatttatttc cgcttgttca agttaaatat ttccgcgcat
    22381 cgattaaaga tgatgcgtcg ttttctgcga tgggaatagt caaaaaagaa aaaccaagtc
    22441 ttttttgatg acaaaatgca atcaaggaaa aattatttta ttttttaact agatacaaca
    22501 atcatcttaa cgaagtatat aatataaatc gtcaacctat gctcatgtta gattattttt
    22561 tgagcgcaca gccgcagcta acaccattgc aattaacaaa tttgcatcaa tccaccatca
    22621 atttacacac attattatgt gataattgcc aaccgctaaa tatgcgtttt gttatctatg
    22681 tataaaaaca gcaacttcaa tgtcttaatg gcagtttttc ttgattttaa tcagcattca
    22741 tcgccaattt attgggcata ttttttcctt aagctttagg aattttttat tatttacttt
    22801 ggggcctgga gacaggaaaa attatgctga cattcattga actccttatt ggggttgtgg
    22861 ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt gtgttatttg
    22921 tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg ttaccgtcca
    22981 gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa atattactaa
    23041 tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt gccgcttaca
    23101 tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca ttgcagtata
    23161 ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg atgtctctgg
    23221 ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt ccggtgatgg
    23281 taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg gcggcgatta
    23341 ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa atgtcgctga
    23401 ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc ggcatggcga
    23461 tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct catgaaatgt
    23521 tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg ccgttcacgc
    23581 cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac atcatcacta
    23641 ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc tttaataccc
    23701 agaaagtttt ctctggtctg gaagtggctt atcgcggaat ggcagatgcg tttgctaacg
    23761 tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc atcggcttta
    23821 ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc ctgatgctgg
    23881 tattggtgat tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt
    23941 atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac ccggcgtatt
    24001 tgactatccc gatgctgcag gcgtcaaacc tgggccgtac cctttcgccc gtttctggcg
    24061 tagtcgttgc ggttgccggg atggcgaaga tctcgccgtt tgaagtcgta aaacgcacct
    24121 cggtaccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg atggtgccag
    24181 gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc
    24241 tttatcatgt cacccactgt ggtatatgcg ttgcggtctg ccaactttgc cgtgaacaat
    24301 ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg cagtggtgcg
    24361 actgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa
    24421 cagttttcgc accgcgttta gcgtcaaggg atcaatgccg gtcggcagtt cgtctttagg
    24481 ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg cgctatcaat
    24541 actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca gccgttcata
    24601 ggcaatgggc ttaatgagat aatcaaatac accacaacgt actgcttcag acaccgtttc
    24661 catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct gcaccagttc
    24721 atgcaataaa ttaatccctc tgccgtcagg aagatagtta tcgagcaaga ttagccccgg
    24781 cttaaaacgc tcgatcatca ttcgggcctg cgccaggttt cccgccagta atatctgact
    24841 gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg tttcgtcctc
    24901 aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata tagattgaaa
    24961 acaaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag cgcgttacgt
    25021 agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca cgcgtactga
    25081 ccccctgctc aaatattttg tctcgtagag actctggaac gccgcagccc tgatcggcga
    25141 cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc ttgtttcctt
    25201 catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgcccaca atggctgcaa
    25261 actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg acaatgatca
    25321 tttttagccc cagttcccgg gcgcgctgca ctttaccaaa aagcagcccc gccacctggc
    25381 gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga gactccccct
    25441 gcaccatcgc cagcacgcga tcatactctt tcatctgcaa cagaccattg agcgtcgaca
    25501 tccaattgag atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt tttatttgcg
    25561 tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata atggccccca
    25621 gcaaatcatc acctgaacga atagcttccc gattggcaat aacgctcaga ccgttaaagt
    25681 tagccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg gcgggcctga
    25741 ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc cccagcatct
    25801 ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca atcagccctt
    25861 cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt tgctttggct
    25921 ccatgcccat catttgccga cggatatgcg ctgccaggaa ccacgacagc aacatcagaa
    25981 tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc cgccagctat
    26041 cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca tcgtcatcaa
    26101 agattggcgt tttggcgcgc atcgccatcc ccattgaccc tttcccggta atgaagtagc
    26161 tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca attttctccg
    26221 gattaggatg gtaaaggcgg atcgagtgcc ggtccccaat caccacataa tcaaaatcgg
    26281 tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc gttttcaccg
    26341 cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc atgtcgcgta
    26401 catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg gctgcaatga
    26461 caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc agtctttgga
    26521 aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg ggttgtcccg
    26581 agcctctttg acggatgtaa ttaaaatgt
//
LOCUS       DAZFJF010000064        26601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFJF010000064 DAZFJF010000000
VERSION     DAZFJF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.064"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            62..493
                     /gene="tnpA"
                     /locus_tag="ACHKAU_002822"
     CDS             62..493
                     /gene="tnpA"
                     /locus_tag="ACHKAU_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO5531848.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(509..697)
                     /locus_tag="ACHKAU_002823"
     CDS             complement(509..697)
                     /locus_tag="ACHKAU_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO5531849.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(701..1060)
                     /gene="yeaR"
                     /locus_tag="ACHKAU_002824"
     CDS             complement(701..1060)
                     /gene="yeaR"
                     /locus_tag="ACHKAU_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO5531850.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1233..1871)
                     /gene="leuE"
                     /locus_tag="ACHKAU_002825"
     CDS             complement(1233..1871)
                     /gene="leuE"
                     /locus_tag="ACHKAU_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO5531851.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1998..2921)
                     /gene="dmlR"
                     /locus_tag="ACHKAU_002826"
     CDS             complement(1998..2921)
                     /gene="dmlR"
                     /locus_tag="ACHKAU_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO5531852.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3024..4109
                     /gene="dmlA"
                     /locus_tag="ACHKAU_002827"
     CDS             3024..4109
                     /gene="dmlA"
                     /locus_tag="ACHKAU_002827"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO5531853.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4360..5970
                     /gene="yeaV"
                     /locus_tag="ACHKAU_002828"
     CDS             4360..5970
                     /gene="yeaV"
                     /locus_tag="ACHKAU_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO5531854.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6002..7126
                     /gene="yeaW"
                     /locus_tag="ACHKAU_002829"
     CDS             6002..7126
                     /gene="yeaW"
                     /locus_tag="ACHKAU_002829"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO5531855.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7182..8147
                     /gene="yeaX"
                     /locus_tag="ACHKAU_002830"
     CDS             7182..8147
                     /gene="yeaX"
                     /locus_tag="ACHKAU_002830"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO5531856.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8201..9316)
                     /gene="rnd"
                     /locus_tag="ACHKAU_002831"
     CDS             complement(8201..9316)
                     /gene="rnd"
                     /locus_tag="ACHKAU_002831"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO5531857.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9398..11083)
                     /gene="fadD"
                     /locus_tag="ACHKAU_002832"
     CDS             complement(9398..11083)
                     /gene="fadD"
                     /locus_tag="ACHKAU_002832"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO5531858.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11288..11869)
                     /gene="yeaY"
                     /locus_tag="ACHKAU_002833"
     CDS             complement(11288..11869)
                     /gene="yeaY"
                     /locus_tag="ACHKAU_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO5531859.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11909..12604)
                     /gene="tsaB"
                     /locus_tag="ACHKAU_002834"
     CDS             complement(11909..12604)
                     /gene="tsaB"
                     /locus_tag="ACHKAU_002834"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO5531860.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12662..14572)
                     /gene="yoaA"
                     /locus_tag="ACHKAU_002835"
     CDS             complement(12662..14572)
                     /gene="yoaA"
                     /locus_tag="ACHKAU_002835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO5531861.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14704..15048
                     /gene="yoaB"
                     /locus_tag="ACHKAU_002836"
     CDS             14704..15048
                     /gene="yoaB"
                     /locus_tag="ACHKAU_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5531862.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            15471..15770
                     /gene="yoaC"
                     /locus_tag="ACHKAU_002837"
     CDS             15471..15770
                     /gene="yoaC"
                     /locus_tag="ACHKAU_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO5531863.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(15890..16069)
                     /gene="yoaH"
                     /locus_tag="ACHKAU_002838"
     CDS             complement(15890..16069)
                     /gene="yoaH"
                     /locus_tag="ACHKAU_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO5531864.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            16143..17504
                     /gene="pabB"
                     /locus_tag="ACHKAU_002839"
     CDS             16143..17504
                     /gene="pabB"
                     /locus_tag="ACHKAU_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO5531865.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            17508..18086
                     /gene="nudL"
                     /locus_tag="ACHKAU_002840"
     CDS             17508..18086
                     /gene="nudL"
                     /locus_tag="ACHKAU_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO5531866.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            18270..19634
                     /gene="sdaA"
                     /locus_tag="ACHKAU_002841"
     CDS             18270..19634
                     /gene="sdaA"
                     /locus_tag="ACHKAU_002841"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5531867.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            19765..21363
                     /gene="pdeD"
                     /locus_tag="ACHKAU_002842"
     CDS             19765..21363
                     /gene="pdeD"
                     /locus_tag="ACHKAU_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5531868.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(21367..22923)
                     /gene="yoaE"
                     /locus_tag="ACHKAU_002843"
     CDS             complement(21367..22923)
                     /gene="yoaE"
                     /locus_tag="ACHKAU_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO5531869.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(22911..23069)
                     /gene="yoaL"
                     /locus_tag="ACHKAU_002844"
     CDS             complement(22911..23069)
                     /gene="yoaL"
                     /locus_tag="ACHKAU_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO5531870.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            23386..24357
                     /gene="manX"
                     /locus_tag="ACHKAU_002845"
     CDS             23386..24357
                     /gene="manX"
                     /locus_tag="ACHKAU_002845"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO5531871.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            24420..25220
                     /gene="manY"
                     /locus_tag="ACHKAU_002846"
     CDS             24420..25220
                     /gene="manY"
                     /locus_tag="ACHKAU_002846"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO5531872.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            25233..26084
                     /gene="manZ"
                     /locus_tag="ACHKAU_002847"
     CDS             25233..26084
                     /gene="manZ"
                     /locus_tag="ACHKAU_002847"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO5531873.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            26139..26597
                     /gene="yobD"
                     /locus_tag="ACHKAU_002848"
     CDS             26139..26597
                     /gene="yobD"
                     /locus_tag="ACHKAU_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO5531874.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         6500 a         6882 c         6608 g         6611 t
ORIGIN      
        1 ctcctgttga ccacctggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca
       61 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac cagataccga ctccagattt ttgaccatga
      181 cgcgacagaa aaactacgca cttacttttc aaatgtatgt gctgattttg aagctgaact
      241 tgttgaaatg gatggcgaac cagatcacgt ccatttgtta attaactatc ctcccaaact
      301 ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg ggtaggttac tgcgacgaga
      361 tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtc ctggctattt
      421 tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc caatacattg aacaacagca
      481 aacacctggt tagatggaaa accgcgcctt atatccccgc cctgaaggac ggggttttac
      541 ggcgcaccgg ataagaacgc acggtattca ccagatcttt tatcacttca gccgccactt
      601 ctggcaccag caaagtcatc ggcgtctctg tttcataatc gacagaaacg ccattgctgt
      661 tattggtgac agtcacggta tacgttgctt tgcccatgat tcatttcccg ttatgaatga
      721 ctttccgttg ttgcgcacct tccatcagga cttcaggagc cacgaagaag tcaatgttga
      781 aataagtatc gtcagtcatg gcttcaatgt tgtgccactt ttctggaggg aacaccgcaa
      841 actgccccgc ttcgataagg atcacctgat caggctctgc actgtgttca tcagcgtagc
      901 cgagatattt gaccgcccca tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc
      961 ctttatcaag atgacgttcg aatattccgg caggcgcagt ttgtttattc cagaaaggcg
     1021 ttgagcgcgt atgaatataa ttctgtggga tttgcagcat cctattccct ccttcgggga
     1081 atgcgctgaa aacggtttat tccagccgtt tcagggtacg cctgataatt tgcattttaa
     1141 ataccattta ttggttactt tttagcacca tatcagagaa aaatcaggga ggattacaag
     1201 tggggagccc gcgacaacgc gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg
     1261 gcgaaaccca cgaacatcaa accaatcagt gagttgccaa ctttagccag tttctttttg
     1321 gtacgtatgt actgcgtgac aaaagcacca gaaataatca ggaagctcaa atagcagaaa
     1381 ctcaccagtt ccagcgtcgt cgccagaata aagaatgaaa ttcccgtatg tggggcatta
     1441 acatcgataa actgtacgaa aaacgacaca tagaacaaaa tggctttcgg attagtcagg
     1501 ctcaaaatta acgcgcgttt aaaaatggcc ccgtattggg gctcatcgga tttggcctcg
     1561 ttatttttac ccttcagggt cgcgtagaga attttactcc ccagatagag caaataaaac
     1621 gcaccaagat aacgtacaat gttgaataat atcggggtgg tcttaattaa tgtcgccact
     1681 ccagcccatg ccagaaacat caatactgca tcgccaataa atacaccgca ggccgcaaga
     1741 taaccgcctt tcataccgct actgacgcta tttttgagta caaacagggt atttggccct
     1801 ggcaccaaca caataaaaat ggccccaacc agataggtcc agtaattcag aaccccgtat
     1861 tcagcgaaca cattaacctc tttaattatc tttcgatcat gcgcgattaa aggtgaatat
     1921 gctaaccaat ctgtagcggc ttagaaagga gaaaatcagg ttttaacccg atatcaaccc
     1981 gataattgaa tcattaatca ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa
     2041 tactggcgta aaaattctac cgttatccgc actttcgctg acgtcgccag ccttgaaaca
     2101 taaacggccc agacgttcgc tggctggtaa tattccggta gcacttgcac taaatgacca
     2161 ctggcaatgt tttcgctaac atcccaccag gagcgcagag caatcccctg cccatcgaga
     2221 caccactgat ggacaatttc tccatgatta gacgacagtg ggccggtgac tttaatcgca
     2281 tggtggcctt ctttattgcg taattgccag acaccaaaag gatgatcgcg ctctttaatg
     2341 accaggcacg gcaatgcgga aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata
     2401 aattccggcg acgcgcagag aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta
     2461 ggggcgatgt catcgccaat gcgaatatcg agatcaacac cttcattgac caaatcgacc
     2521 aaccggtctt ccacatcaaa acgtaattcc agttgcggat acgccttagc cagcgctgat
     2581 aacgccggag ccaccacctg tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc
     2641 tgcggcacct gacgcacgtc agaaagttcg tccatcatct gaccgacatc ctgcaaaatc
     2701 cgctgcgccc attcataaat ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg
     2761 cggtgtaaca acaccacgtt tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac
     2821 gccggtgaaa cgcccagttc ttccgccacg gcggcaaaac cagcccgacg agccaccagc
     2881 ataaagacgc gcaaatcatt cagcagcggt aaattattca tgattcgtgt tttatgtttc
     2941 accagttacg gggattaatt ccttttcagt caattatagg atggtgatgt tgtcaatttt
     3001 gatggtcagg aagtgagaac ccaatgatga aaacgatgcg tattgctgcg atcccgggag
     3061 acgggattgg caaagaagtc cttcctgaag ggattcgcgt gttacaggct gccgctgagc
     3121 gctggggctt cgccttgagt tttgagcaaa tggagtgggc gagctgcgag tattacagcc
     3181 atcacggtaa aatgatgccg gacgactggc atgagcaact tagccgtttc gacgccatct
     3241 attttggtgc cgtcggctgg ccggacaccg ttcctgacca tatttcgttg tggggttcgc
     3301 tgctgaaatt tcgtcgtgaa ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc
     3361 ctggcgttcc ctgcccgctg gcgggaaaac agcctggcga catcgatttt tacgtggtca
     3421 gggaaaacac cgaaggcgaa tattcctcgc tcggcggtag agtgaatgaa ggtacagagc
     3481 atgaagtcgt cattcaggaa tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt
     3541 atgccttcga acttgcgcaa agccgcccac gtaagacgct aacttctgcc actaaatcaa
     3601 acggtttagc catcagcatg ccgtactggg atgagcgagt ggaagcaatg gccgagaatt
     3661 acccggagat ccgctgggac aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc
     3721 agccggaacg cttcgatgtg gtggtggcgt ccaatttatt tggcgatatc ctttccgatc
     3781 ttggcccggc ctgcaccggc accattggca ttgccccatc cgccaacctg aatccggaac
     3841 gcactttccc gtcgctcttc gagccagtcc acggttccgc gccggatatc tacgggaaaa
     3901 acattgctaa ccctatcgcc acgatttggg ccggtgcaat gatgctcgat tttctcggca
     3961 atggcgatga gcgtttccag caagcgcata acggtattct ggcagcaatt gaagaagtga
     4021 ttgctcacgg gccgaaaaca cctgatatga aaggcaatgc caccacgcca caggttgccg
     4081 acgcgatttg caaaattatt ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact
     4141 gacgcggttt ttttattcgt tctttgcagt aaataacctg cgtcatttca ccttttattg
     4201 tttccgtttc gtgttttatg gctttccgta ttcttaattg tttaatttat gtaacatgca
     4261 aataatcaat ttgttaaaca caacatacaa ctcattaccc tgcaaataat tacaactggc
     4321 aaaagccaga gacgggcttt tatgcccagg agatgactca tgatgagcaa tgttaagaaa
     4381 aaagatgtgc cgctgataag catcagcctg gtggccattc ttttcatcgc agctgcatta
     4441 agccttttcc cacaacaatc ggccgacgcg gccaacgcaa tatacacttt tgttacgcgt
     4501 acgttaggtt ccgccgtaca ggtattggtt ttgctggcaa tgggactggt gatttattta
     4561 gccaccagta aatacggcaa tattcgtctt ggcgaaggaa aaccggaata cagcacgctc
     4621 tcctggctgt ttatgtttat ttgtgccggt ttaggttctt ctacgcttta ttggggggtt
     4681 gctgaatggg cctattatta tcaaacgcct ggattaaata tcgcaccgcg ttcacaacag
     4741 gcactcgaat ttagcgtccc ttactccttc ttccactggg gcatcagcgc ctgggcaact
     4801 tatacgctgg cctcattaat catggcttat cactttcatg tgcgtaaaaa caaaggtctg
     4861 agtctttccg gcattattgc cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa
     4921 ctggttgatt tgatgttcct gatcgccact gtcggcgcac tgactatttc ccttgttgtc
     4981 accgcagcaa cctttactcg tggactttcc gcgctgaccg gtttacccga taacttcacc
     5041 gtgcaggcat ttgtgatcct gctttccggc ggcatttttt gcctaagctc gtggattggt
     5101 atcaacaacg gtttgcaacg tctgagcaaa atggttggct ggggcgcgtt cctgctgcca
     5161 ttactggtgc tgattgtcgg cccaaccgaa tttattacca acagcatcat caatgccatc
     5221 ggtctgacca cgcaaaactt cctgcaaatg agcttattca ccgatccgct tggcgatggt
     5281 tcatttaccc gcaactggac cgttttctac tggctgtggt ggatctcata cacccctggc
     5341 gtagcaatgt ttgtcacccg cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga
     5401 ctgatcctcg gcagcaccgt cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc
     5461 attcatcagt ttatcaatgg cgtaatcaac gtcccacagg tgctggaaac actgggcggc
     5521 gaaacagctg tacagcaagt tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca
     5581 tacctgggcg tgatgattat tttccttgcc tcgcatatgg atgcggtggc ctacaccatg
     5641 gctgcgacca gtacgcgtaa tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt
     5701 ttctggtgcg tggtgatcac tctgatcccg ctttccatct tgtttaccgg tgcttcgctg
     5761 gaaacgatga aaaccaccgt cgtgctcaca gcccttccct tcctcgtcat tttactggtg
     5821 aaagtcggcg gatttattcg ctggctgaaa caggattacg ccgacattcc ggctcatcaa
     5881 gttgaacatt atctcccgca gacaccggtt gaagccctgg aaaaaacgcc agtgctccct
     5941 gcgggaaccg tattcaaagg cgacaactga gcgccatcat cctaacgata aaggtatccc
     6001 tatgagcaat ctgagccctg actttgtact acccgaaaat ttttgcgcta acccgcaaga
     6061 ggcgtggacc attcctgccc gtttttatac cgatcagaac gcgtttgaac acgaaaaaga
     6121 gaacgtcttc gccaaaagct ggatttgcgt cgctcacagc attgaactgg cgaatgccaa
     6181 tgattatgtg acgcgtgaga tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa
     6241 ggttttgcgc gcgttctata acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga
     6301 aggaaaagca aaaaatgtga ttacctgccc gtatcacgca tgggcattca aactcgatgg
     6361 caatctggcc catgcacgta actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca
     6421 actggttccg gtgcgtctgg aagaatatgc cggattcgtc ttcatcaaca tggaccccaa
     6481 cgccaccagc gtagaagatc agttacccgg tctgggcgcg aaagtgctgg aagcctgccc
     6541 ggaagtccac gatctgaaac tggcggcccg ctttaccacc cgcacgcctg ccaactggaa
     6601 gaacattgtc gataactatc tcgagtgcta tcactgtggt ccggcgcatc caggtttctc
     6661 cgactccgta caggttgatc gttactggca caccatgcac ggtaactgga cgctgcaata
     6721 cggtttcgcc aaaccgtccg aacagtcgtt taaatttgaa gagggtacgg atgcggcatt
     6781 ccacggtttc tggctgtggc cgtgcacgat gctgaacgtc accccgatca aagggatgat
     6841 gacggtcatt tatgaattcc cggtggattc tgaaactacc ctgcaaaact acgatattta
     6901 cttcaccaat gaagagttaa ccgacgagca aaaatcgctg attgagtggt atcgcgatgt
     6961 gttccgtccg gaagatttac gtctggttga aagcgtacag aaagggctga aatcgcgtgg
     7021 ctatcgtggt caggggcgca tcatggccga cagtagcggt agtggcattt ccgaacatgg
     7081 tatcgcccat ttccataatc tgctggcgca ggtgtttaag gactaatgac atcggcggcg
     7141 gtattttccg ccgctgggct gatttttgat ggagtacagc aatgtcagac tatcaaatgt
     7201 ttgaagtaca ggtgagtcag gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg
     7261 tggcaaccga tggcaaaccg ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga
     7321 tgagcgatgg tgataaccag tacagcaatg cgtattcact actgagttcg ccgcatgaca
     7381 cctcttgtta tcagattgcc gttcggctgg aggaaaactc gcgcggcggt tcccgctttt
     7441 tgcatcagca ggtaaaagtg ggcgatcggt taacgatttc aacgcctaat aacctgtttg
     7501 cgctaattcc ctcagccaga aagcatctgt ttatcgcggg cggtattggt atcacccctt
     7561 tcctgtcgca catggcagag ctgcaacaca gcgacgtcga ctggcagcta cattactgct
     7621 cgcgaaatcc agaaagttgc gcatttcgtg atgagctagt ccagcatccg caggctgaga
     7681 aagttcactt gcatcactca tcaaccggaa cacgactgga attagcgcga ttattggcgg
     7741 atatcgaacc tggcacacac gtttatacct gtggtcccga ggcgctaaat gaagcggtaa
     7801 gaagtgaagc tgcgcgtctg gacatcgtcg ccgatacgct gcactttgag caatttgcta
     7861 tcgaagacaa aaccggcgat gcctttaccc tggtgcttgc ccgttccgga aaagagtttg
     7921 tggtgccgga agagatgact attttgcagg ttattgaaaa taataaagcc gcgaaagtgg
     7981 aatgtttatg tcgtgaaggg gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag
     8041 ctgaccatcg ggatcagtat tttagcgatg aagagcgtgc cagccagcaa agtatgttga
     8101 tctgttgttc gcgtgcgaag ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg
     8161 gatgtggcgc tgagcgcgcc cagtccggct tcggaagatt ttactgcgga tattcctgca
     8221 ataaattgtg taatgcttcc gccatcagct caccacgcca gccggaaatc agcgccggca
     8281 aattgttctg cggtttcagt ttccagtgcc agttcagcag ttggttgatt tgccgacgcg
     8341 atgccagcaa ttcggcgctg atcttatgcg tttcgctcac gtcagtaatc agcgacttaa
     8401 tcgctttaaa cactttacga taaccaggca tgtccatcag gttaagcatc ggctgcggta
     8461 aggcctcttc cggcaatgtc tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa
     8521 agcggatttc gctaccggat aaacccaggc tgtccagttc gcctaaactt cccggcatat
     8581 aacgcgcgac cgaccacaaa tgctcttcac gcacgacaaa gttcaccgcc agatcacgct
     8641 ctcgcgcctt gcgcagtcgc cagtcggcta acagttgcag acaggccagt tggcgtgtgc
     8701 gtaactgcca ggcattggtg atatcacgcc aggcatcttc cggcgcaacg acttcctgac
     8761 gacgcatttg catcaggcgg cattcatcca gcgccgcagg tagccagccg gaggcctccg
     8821 tttctaccat aagcttggcg gtgatcggta acagatacca gacatccgcc gctgcgtatt
     8881 cacactgacg ttcggtcagc ggtctggcca gccagtcggt gcgcgattca ctcttgtcca
     8941 gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc
     9001 agaaggcagc aaggatttgc gtgtcaatca agggttgtgg taattcgcca aagacattga
     9061 ggaacacttc cagatcttca ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca
     9121 ggatcgcttt cagcggtgac cagtcggtga tcccgagagg atcaattagc gccagatgct
     9181 cgccatcgaa aagttgaatc aaccccagct gcgggtaata agtgcgcgta cgaacaaatt
     9241 cagtatccag ggctatcgcc ggaaaggcac ggacggcttc acacaaagaa gccagcgcat
     9301 cgtccgtggt aatcatttgg taattcaaat tgttttctct ttagtgggcg tcaaaaaaaa
     9361 cgccggatta accggcgtct gacgactgac ttaacactca ggctttattg tccactttgc
     9421 cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc gacgttagat ttcggtaact
     9481 catcacgaaa ctccaccagc ttcggtactt tgtagcccgt gagctgacgg cggcaaaagg
     9541 tcaccagtga ctcttcggta agcgatggat cttttttcac tacgaagatt ttcaccgctt
     9601 caccactgga gccggaaggt acgccaacag ccgcgacttc ctgtacgcca ggatgctgca
     9661 tgacgacatc ttcaatctcg ttgggataga cgttaaaacc ggaaaccaga atcatgtctt
     9721 ttttacgatc gacaatgcgc aggaaccctt cttcatccat caccgcgatg tcgccggtgt
     9781 gtaaccagcc atttttgatg atctcatctg tagcatccgg acgctgccag taacccagca
     9841 tcacctgcgg tcctttgaca caaagctcac ccggttgacc cggtggtact tcattatcat
     9901 catcatccac cagtttggct tccgtcgacg gcaccggcaa accgatgcta ccactatgat
     9961 aatcaatatc atatgggtta acgctgacca gcggcgcaca ctcggtaagg ccatagcctt
    10021 ccagcagata ctgtcctgtc agtttcaccc aacgctctgc caccacttgc tgcactggca
    10081 tccctccgcc tgcggaaaga tgcagactgg agaaatccag ctgctggaac tctttattgt
    10141 tcagcaacgc attgaacaag gtgttaacgc ccgtgatagc ggtaaacgga tatttcgcta
    10201 actcttttac caaccctgga atatcgcgcg ggttagtgat aagcaggttc tgcccaccca
    10261 gttcgataaa cagcaggcag ttaatggtca gggcaaaaat gtgatacagc ggcagcgccg
    10321 tcaccaccag ctctttgccc ggatgcaaca gcggaccata ggtcgcgtta acctgttcca
    10381 ggttcgccag catattgcgg tgagtcagca tcgcgccttt cgccacacca gtggtgccgc
    10441 cggtgtattg cagaaaagct aaatcttccg gcaccagttc gggtttgacg tactgcatcc
    10501 ggtagccgtt atgcagtgcg ctacgaaatg aaatggcatc tggcagatgg tatttcggca
    10561 ccaaacgctt gatgtattta acaacgaaat tgactaccgt gccttttgcc gtagatagct
    10621 gatcgcccat acgggtcaga attacgtgct gaacggcggt tttatcaacc actttttcca
    10681 gtgtgtgagc aaagttagac acgataacaa tcgccgatgc gccgctatcg ttaagctgat
    10741 gctcaagctc acgcggggta tacaacgggt taacgtttac gacgatcatc ccggcacgca
    10801 aaatgccaaa cagcgccacc ggatattgca ataaattagg catcatcaac gcaacgcgat
    10861 cgcctttctt cagccccaac ccttgttgca aataagcggc aaacgcgcga ctgcgttctt
    10921 ccagcttgcg gaaggtcatt acctccccca tattcacaaa cgcaggttga tcggcgtagc
    10981 gcgcgaccga ctgctcaaac atatctacca gagattgata acggtcaggg ttgatctccg
    11041 tcggaacgtc cgcgggataa cggttaagcc aaaccttctt caattcttca cctctaaaat
    11101 gcgtgttcgt cgtcatcgca accccaaatg atatacatgc cgttaacata atattaactc
    11161 atcataccag cttgataatt acccaacgaa aaggttgcga agcgcgtcac tatttatttt
    11221 tatctttacc gtaagaatgc agaaacagcg gaccagccgc tgtttctttt tctttaaaaa
    11281 caagcaatta ttcagttaca actgtttgta ctctcgcggg gccgggatta taccagcccc
    11341 atccgccgta gccatagggc cagccacgac cgccataaaa ccatggatca atcggctgag
    11401 gcggcataat cacctgctgg gttaaatgcc aacgtttgta acccgttact tgcatcacca
    11461 taaatttata gggcgtattg ccgattttgc cgtcaaccgc accagtgatt ggcccgacta
    11521 ccgtaacaag ttgtccacgg aaatccaccg ggtccagaaa accgttcaca tcggcataaa
    11581 tgcgaccgcg agaaggttct cccagcgtcg gtctggctcc gctgtccagc ggtacggtag
    11641 caatttccag gcgggttttc ccttgctggt tttgtaccgc aaccactttg ccaccaaagc
    11701 gtgcctcctg accaacgtac agctgcggcg cactcatcac ccgaactaaa tcttgttgcg
    11761 gcgtgggact gctgccttta atggcgtccg gcacagtgac acaaccgctc agcattagcg
    11821 caaacgtacc tgccagtatg cctttgataa cattcttttg aaccgccatg atgcgactcc
    11881 tttttttctc agggcatact cttaagattc attctttgcc cggaagtttc ttccatgcga
    11941 cgttgttacg taaataaacc ggttcggcat gttccaccgc caccgtttta ccctcggcaa
    12001 acatctgaca cgcaatcggc agcatatctt ccgccgctgg cagtaaaact tctccgtcgc
    12061 gcaaaaccag cccgctctct ttaccgagat ccggccaggc ttgccagccc gtccctaccg
    12121 ttacccattc gccggaaagc tgttgcattc gttcatgaac gagttcgggt ttgagtacgg
    12181 cttcggtttc ttcaccgcgc cagataccgt tttcatcacg ctgatattcg gcccagtaaa
    12241 cttcgcccat tcgcgcgtca atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg
    12301 ccccttgcgc catcgtcatt agcgtggaga cgccaatcat cggtagctct gcgccaagtg
    12361 ccagcccttg cgcaatgcca ataccaatgc gcacgccggt aaagctaccg gggccgcgcc
    12421 cgtaaaccag agcgttaata tcagtcaggg aagttccgct ggtggtcagg atatcctgca
    12481 ccatcggtaa gattcgttga gtatgttcac gagggcaaag ctcaaaatga gcgttgacag
    12541 taccgtcgtt ccacagggcg acagagcagg cctctgtcgc ggtatcgata gccagaattc
    12601 gcatgggtct tcgtgcttag atcaataaaa aggcgcgcat cataccatac tccgtaacaa
    12661 attacctgga ggatggtatc gcaaggaaac gaaccgcacg ggcaatgtca cgagtgcgtg
    12721 gcgcgggcgg cagactggcg agaaacgtcg cgccgtaagg acgcatcacc agccgattgt
    12781 cacaaatcac cagcacgcca cgatcgtcgg cgtcgcgaat cagtcgccct accccctgtt
    12841 tgagagtaat gacggcatct ggtagttgca cttcatcgaa cgggtcgcca ccgcgcaaac
    12901 gacaatcttc catgcgcgct tttaacagtg gatcatccgg cgaggtaaac ggcaacttgt
    12961 cgataatcac caatgacaat gtatcgccac gcacgtccac cccttcccag aaactactgg
    13021 ttgccacaag aagcgcgtta ccggcgctga caaattgctg caacagttgc cctttgctgg
    13081 tttccccctg caacaaaacg ggaagcgtca tggtagcgcg gaactgctcg gccagatcac
    13141 gcatcatggc gtgcgaggta caaagcataa aacaacgacc gttgttagct tcgatgatcg
    13201 gtcgcagcat tgccgctaac tggcgagcag aacctggctg gttggtttgc ggcagattgc
    13261 gcggcacaca gagtaacgcc tggcggctgt aatcaaacgg gcttggcaat agcaacgact
    13321 ccgcctgttc tatcccaagc cgcgaggtga aatgatgcag atcgtcgttc accgacagcg
    13381 ttgctgaggt gaagatccag ctaccaggct tttgcgccat taactctttg aatttatccg
    13441 ccacgctgag cggcgtgaga gccagagtaa aatggcgcga agtgcattcg taccagtagc
    13501 tgtagcccgg ctgattgatc tcttttagcc gcttcagccg tgtgcgatac aacgtagcgc
    13561 gctcaaatgc cgcatccagc aaggcggaac gccccagtga cagtttcgcc acgtcataac
    13621 aaagttccag ggtgtcatcg agcagtaaaa atgcccgctg aatttgcgga ttagctaaca
    13681 gctcacgcag gttaccgcga taacctggct caccgagttg cagacgaaaa tcctgcgcac
    13741 tctgggcaag acgatcagcg cacttttgta actgctgggt gtcttttaat tcggtgcggt
    13801 aagcgatggt gatgtctttt gccaggtcga gcagttgtcg actggagagt gactgaccaa
    13861 aatactggct ggcaatgtcc ggtagctggt gggcttcgtc gaagatcatg acgtccgctt
    13921 ccgggatcag ttcgccaaat ccactctctt taaccaccat atccgccaga aagagatgat
    13981 ggtttaccac caccacatcg gcgtccatcg cttttttacg tgctttgacc acaaagcaat
    14041 ctttatacat cgggcagtcg ctgccaagac agttgtcgtt ggtgctggtg accagcggcc
    14101 acgcctgtga atcttccgcc acgctgacgc aggtgctgat atcaccatcg actgtttgat
    14161 tagaccagga gcgcagcagg atcacatcgc ttaagatttg taccggcaga tcgccccccg
    14221 ccagcgcctg ctgttcgaga cgctcgaggc agaggtagtt tgagcgccct ttcagcagcg
    14281 ccacgttgcc cgtatatttc aatgcctttg agactgttgg caaatcgcgg ctgtagagct
    14341 gatcctgcaa cgcttttgag ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag
    14401 gagccaggta agcgtaggtt ttgcccgtac cggttcctgc ttccaccacc agcggctggc
    14461 ctttttctat cgcctgggtg acggctaccg ccatctgtcg ctgtggttct cgcggcttaa
    14521 agcctggtat cgctttcgcc agctgaccgt ctggtgcaaa atcgtccgtc acactacccc
    14581 ctgttgattt gaacagggat tatgtcagga tgagggcgct ttcgccagtt gaattggtga
    14641 cggcgacctc acattgtggc agtctttgca gcacgaaaat ggaaggataa tgaggacaaa
    14701 atgatgacta tcgttcgtat cgatgctgaa gcccgctggt ctgatgtggt aatccacaac
    14761 aacacgctct actacactgg tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc
    14821 gccaacacgc tggcgcagat tgatgccgtg ctggaaaaac agggcagcaa taaatcgagc
    14881 attctggatg ccaccatttt cctggccgat aaaaacgact tcgcggcgat gaataaagcg
    14941 tgggatgcct gggttgtcgc gggtcatgcg ccggtgcgct gcacggtaca agcgggtttg
    15001 atgaacccga agtataaagt tgaaattaag attgtggctg cggtgtaagc tttatcgaag
    15061 caaaataagt cagacgataa tttatcgata atactggtcg gttttacata aatcgaccag
    15121 agattaagat attacctgtt gatgctcctg ctaattcatt atttattttt ataatttcaa
    15181 ttttatctat aaaactaact atataaataa ccataaccat gccgttgtgt tcgccctctg
    15241 actagcaact ctggttttct ttcttagctg aataatctgg ttatagcgtg aggactgacc
    15301 gttaaatttc tccatcttac tggataacac accatgagct gctaccttta aaacacctga
    15361 atgtcaacag gttaactcgt gccatatcgt tataatccct ttgatattag atgcaaatta
    15421 aggtcatata tagtcttatc gataacatgg ttaattttaa ggataagaat atgcctgctg
    15481 taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca acaccaagtt
    15541 cgatgaatga aagcacggcg aagggaatct ttaaatattt aaaagaactg ggagtacccg
    15601 cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct gggttcacgg
    15661 aaaaaatggt tggatgggca aaaaaaatgg agacaggtga acgctctgtg attaaaaatc
    15721 ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtctga gttaaattta
    15781 tatcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc tttttctcac
    15841 aatcaaatga tcatcggctt ctataacgaa atcgatacgg attagtgtgt tactcgtctt
    15901 catcctcaaa acgcgccacg attcgctcgc cggagtggtt ggcacgcagc tcttctgcca
    15961 ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg atccgctcga
    16021 cagctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt gttaactcct
    16081 gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta gagtcaggac
    16141 tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac gccgctgaat
    16201 tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc ggctatgccg
    16261 atcatccgta tagccgcttt gatattgtgg tcgccgatcc gatttgcact ttaaccactt
    16321 tcggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact gatgacccgc
    16381 tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccaacgcat aacgaagatt
    16441 tgccatttca gggcggcgca ctggggttgt ttggctacga tctgggccgc cgttttgagt
    16501 cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg ggtatctacg
    16561 attgggcgct cattgtcgac caccagcgtc atacagtttc tttgctgagt cataatgatg
    16621 tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa gatttcacgc
    16681 tcacttccga ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa tttcgccagg
    16741 tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag cgttttcatg
    16801 cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc aaccgcgcgc
    16861 catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg ccagagcggt
    16921 ttattctttg tgataatagt gaaatccaga cccgcccgat taaaggcacg ctaccacgcc
    16981 tgcccgatcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca gcgaaagatc
    17041 gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt gttgccgtag
    17101 ccggttcggt aaaagtacca gagctcttcg tggtggaacc cttccctgcc gtgcatcatc
    17161 tggtcagcac tataacggcg cgactaccag aacagttaca cgccagcgat ctgctgcgcg
    17221 cagcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg gaaattatcg
    17281 acgaactgga accgcagcga cgtaatgcct ggtgcggcag cattggctat ttgagctttt
    17341 gcggcaacat ggataccagc attactatcc gcacgctgac tgccattaac ggacaaatat
    17401 actgctctgc gggcggtgga attgtcgccg atagccagga agaagcggaa tatcaggaaa
    17461 cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg gaataccgta
    17521 gcctgacgct tgatgatttt ttatctcgct ttcaactttt gcgcccgcaa attaaccggg
    17581 aaaccctaaa tcatcgtcag gctgccgtgt taatccccat cgtccgtcga ccgcaaccgg
    17641 ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa gtggcattcc
    17701 ctggaggtgc agtcgatgac acggacgcat cagttatcgc cgccgcgctg cgcgaagctg
    17761 aagaagaggt cgctataccg ccttccgccg ttgaagttat cggcgtgctg ccgcccgtcg
    17821 atagcgtaac tggctatcag gtaaccccag tggtcggcat tatcccgccc gatctgccgt
    17881 atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc caggcattac
    17941 atctgggtcg ttatcaccct ttagatatct accgccgtgg cgattcacat cgggtatggc
    18001 tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt cgtgagctgg
    18061 cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa aacactactt
    18121 gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat tcatgtgaat
    18181 agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt cgttactgga
    18241 agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg tttaaggtgg
    18301 ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa cagttcgtcg
    18361 atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg gacgtttatg
    18421 gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt atgggtcttg
    18481 caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt cgcgacgtag
    18541 aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc ccgcgcgaca
    18601 acgggatgcg ttttcataac ggcaacctgc cgctgcatga aaacggtatg caaatccacg
    18661 cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc ggcggtttta
    18721 tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg ccgtatccgt
    18781 tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggctattcg ctgtctggtc
    18841 tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag tatttcgcgc
    18901 atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa ggtgtactgc
    18961 caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgtta gtttccagcg
    19021 ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt gcgctggcag
    19081 ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac ggtgcctgcg
    19141 gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcggtc agcccggaca
    19201 tctatacccg ttactttatg gcagcgggcg cgattggtgc attgtataaa atgaacgcct
    19261 ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt tcaatggctg
    19321 ctgcgggtct tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg gcggcggaaa
    19381 ttggcatgga acacaacctt ggtttaacct gcgacccggt tgcagggcag gttcaggtgc
    19441 cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat taacgccgca cggatggctc
    19501 tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg atgtacgaaa
    19561 ccggtaagga catgaacgcc aaataccgcg aaacctcacg cggtggtctg gcaatcaaag
    19621 tccagtgtga ctaatacttc ttactcgccc atctgcaacg gatgggcgaa tttatacccg
    19681 ctttctcgtc tgctgtaata ttccccacta cacttccact gttgcgtcag gcgtttgtcg
    19741 ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa aacctatcgc
    19801 cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc gaccctgagc
    19861 gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt cgccaatcac
    19921 gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga agtcttgctt
    19981 ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca ggcggcaaaa
    20041 ctccaaactg tgcgatccat tggcctggtg caagacggca cactctattg ctccagcatt
    20101 tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc accgcaacca
    20161 cttttacgcc tgacgatcga ccgtgccctg attaaaggca gtccggtttt gattcaatgg
    20221 acgcctgcag cgggcagtag caatgctggg gtcatggaga tgattaacat cgacttactg
    20281 acggcaatgc tccttgagcc acaactgccg caaatcagta gcgccagcct gacggtggac
    20341 aaacggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc tgaagacaat
    20401 gaaaactatc aggtttcttc gcaacgcttt ccttttacca ttaacgttaa tggtccgggg
    20461 gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt gctgctaagt
    20521 ttactggtgg gctacatcgc ctggctggcg accgcttacc ggatgagctt ttcccgcgaa
    20581 atcaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt gcttaatgcg
    20641 cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaataatcc gcgtcagggc
    20701 tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat tgtgccactg
    20761 acccgctatg tgatggcaga aaccattcgt cagcgccatg ttttcccgat gagtagtcag
    20821 tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct gataaaagat
    20881 ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga aatcaccgaa
    20941 cgcgatgcct tactggatgt tgattatcgg attgcccgcg agctacatcg taaaaacgtc
    21001 aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct tgaaacatta
    21061 cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc tgacgcggtt
    21121 aactcgacgg tgaccgatat catcatcgcg ctggggcaaa gactgaatat tgaactggtg
    21181 gcggagggcg tggaaacgca agaacaggcg aagtatttgc gccgtcatgg cgtgcatatt
    21241 ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa atggctggcg
    21301 ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccattat gccgttacgt
    21361 taatgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa ccagatcaac
    21421 acgataatca ttggcttcaa tgatggtgat atgcagtggc cctacatcaa tcacatcgcc
    21481 cacacgggga atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg cgatatcatc
    21541 gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat ctgtaccgcc
    21601 ttttaccagc cagccgtcac catcagtaat gatttccggc gtttcgtcag cgtccgggaa
    21661 ttcacccgca atggcttcca gcacatccag cggcgtgacc agaccttgta ccacaccaaa
    21721 ctcgttggtc acgataacaa agctcccgcg agcacgacgc agcacgccca acaggttgat
    21781 cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagcaatcg ccgccacatc
    21841 aacgccctct tccagcgcca ccagcagttc tttagcacgt acaataccga tgatttcatc
    21901 cagttcaccg cgacataccg ggaacagact gtgcggtgaa gagagcagtt gctcgcggat
    21961 ttcatcgacc ccgagattag cgtcaaccca gcttatttca ccgcgcggcg tcatgatccc
    22021 gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt cttcttcggc
    22081 aaatgcacct tccgggatcg gcatcggcat cgggttatcg gcatcgtgct gaacattggc
    22141 ctgacgtttc ccgcccatca aacgcaggat ggcatcggca gtacgcgctc gcagcggcaa
    22201 agtcgactgg tggcgaataa agttgcgacg cgcaatctgg ttaaacactt cgatgatgat
    22261 cgagaagcca atcgcggcat acaggtaacc tttcggaatg tggaaaccga aaccttctgc
    22321 caccagactc agaccaatca ttaacaggaa gctcagacag agcaccacca ccgtggggtg
    22381 ctggttaacg aatcgcgtca gcggtttgga tgccagcaac ataaccgcca tcgcaatcac
    22441 taccgccgcc atcatcaccg gcagatggtt aaccatccct actgcagtaa ttaccgcatc
    22501 caacgagaag acggcgtcaa ggatgacaat ctgtgtgacg accacccaga aactggcgta
    22561 gcctttaccg tggccggaat catgatcgcg gttttccagc cgttcatgca gttcggttgt
    22621 tgctttgaac agcaagaata tccccccgaa caacataatc aggtcgcgtc cggagaagga
    22681 gaaatccatg acggtaaata gcggtttggt cagcgtgacc atccatgaaa tcagcgacag
    22741 cagccccaga cgcataatca gcgccagtga taaccccagc aaacgcgctt tatcgcgttg
    22801 ttttggcggc agtttgtcag caagaatggc gatgaagacc aggttatcga tacccagcac
    22861 aatttcgaga acaacaagcg tgagtagccc cgcccaaatt gaggggtcca ttaagaattc
    22921 catgacaagc tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc aggcgtaacg
    22981 acaaaatgca aacgaaaggt gcggcataga gagccagaaa ggtaggcgtt aaaaggcctg
    23041 atgctgaaat gacgtcggtg acgatccata ctgcgggcta ctgccctata ctccatggtt
    23101 gttaaacggg agttaaacat atcagagacg cctctgattt ggcaaagatt taccttcctt
    23161 tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt tctgctaatc gaaagttaaa
    23221 ttacggatct tcatcacata aaataatttt ttcgatatct aaaataaatc gcgaaacgca
    23281 ggggtttttg gttgtagccc ttatctgaat cgattcgatt gtggacgacg attcaaaaat
    23341 acatctggca cgttgaggtg ttaacgataa taaaggaggt agcaagtgac cattgctatt
    23401 gttataggca cacatggttg ggctgcagag cagttgctta aaacggcaga aatgctgtta
    23461 ggcgagcagg aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc cgaaacgctg
    23521 attgaaaagt acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt gctgtttctc
    23581 gttgatacat ggggaggcag cccgttcaat gctgccagcc gcattgtcgt cgacaaagag
    23641 cattatgaag tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt aatggcccgt
    23701 gatgatgacc caagctttga tgaactggtg gcactggcag tagaaacagg ccgtgaaggc
    23761 gtgaaagctc tgaaagccaa accggttgaa aaagccgcgc aagcacccgc tgccgcagca
    23821 ccaaaagcgg ctccaactcc ggcaaaacca atggggccaa acgactacat ggttattggc
    23881 cttgcgcgta tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg gaccaaagaa
    23941 accaatgtct cccgtattat tgttgttagt gatgaagtgg ctgcggatac cgttcgtaag
    24001 acactgctca cccaggttgc acctccgggc gtaacagcac acgtagttga tgttgccaaa
    24061 atgattcgcg tctacaacaa cccgaaatat gctggcgaac gcgtaatgct gttatttacc
    24121 aacccaacag atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc tgttaacgtc
    24181 ggtggtatgg cattccgtca gggtaaaacc caggtgaata acgcggtttc ggttgatgaa
    24241 aaagatatcg aggcgttcaa gaaactgaat gcgcgcggta ttgagctgga agtccgtaag
    24301 gtttccaccg atccgaaact gaaaatgatg gatctgatca gcaaaatcga taagtaacgt
    24361 attgtgttga ttatcactca gttttcacac ttaagtctta cgtaaacagg agaagtacaa
    24421 tggagattac cactcttcaa attgtgctgg tatttatcgt agcctgtatc gcaggtatgg
    24481 gatcaatcct cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc ctggtgggta
    24541 tcgttcttgg ggatatgaaa accggtatta ttatcggtgg tacgctggaa atgatcgcgc
    24601 tgggctggat gaacatcggt gctgcagttg cgcctgacgc cgctctggct tctatcattt
    24661 ctaccattct ggttatcgca ggtcatcaga gcattggtgc aggtatcgca ctggcaatcc
    24721 ctctggccgc tgcgggccag gtactgacca tcatcgttcg tactattacc gttgctttcc
    24781 agcacgctgc ggataaggct gctgataacg gcaacctgac agcgatttcc tggatccacg
    24841 tttcttctct gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc gttgcgctgt
    24901 ctgttggtac cagcgaagta cagaacatgc tgaatgcgat tccggaagtg gtgaccaatg
    24961 gtctgaatat cgccggtggc atgatcgtgg tggttggtta tgcgatggtt atcaacatga
    25021 tgcgtgctgg ctacctgatg ccgttcttct acctcggctt cgtaaccgca gcattcacca
    25081 actttaacct ggttgctctg ggtgtgattg gtactgttat ggcagtgctc tacatccaac
    25141 ttagcccgaa atacaaccgc gtagccggtg cgcctgctca ggcagctggt aacaacgatc
    25201 tcgataacga actggactaa caggtgagcg aaatggttga tacaactcaa actaccaccg
    25261 agaaaaaact cactcaaagt gatattcgtg gcgtcttcct gcgttctaac ctcttccagg
    25321 gttcatggaa cttcgaacgt atgcaggcac tgggtttctg cttctctatg gtaccggcaa
    25381 ttcgtcgcct ctaccctgag aacaacgaag ctcgtaaaca agctattcgc cgtcacctgg
    25441 agttctttaa cacccagccg ttcgtggctg cgccgattct cggcgtaacc ctggcgctgg
    25501 aagaacagcg tgctaatggc gcagagatcg acgacggtgc tatcaacggt atcaaagtcg
    25561 gtttgatggg gccgctggct ggtgtaggcg acccgatctt ctggggaacc gtacgtccgg
    25621 tatttgcagc actgggtgcc ggtatcgcga tgagcggcag cctgttaggt ccgctgctgt
    25681 tcttcatcct gtttaacctg gtgcgtctgg caacccgtta ctacggcgta gcgtatggtt
    25741 actccaaagg tatcgatatc gttaaagata tgggtggtgg cttcctgcaa aaactgacgg
    25801 aaggggcgtc tatcctcggc ctgtttgtca tgggggcatt ggttaacaag tggacacatg
    25861 tcaacatccc gctggttgtc tctcgcatta ctgaccagac gggcaaagaa cacgttacta
    25921 ctgtccagac tattctggac cagttaatgc caggcctggt accactgctg ctgacctttg
    25981 cttgtatgtg gctactgcgc aaaaaagtta acccgctgtg gatcatcgtt ggcttcttcg
    26041 tcatcggtat cgctggttac gcttgcggcc tgctgggact gtaagactgt tgtacactac
    26101 cggggccttt tggccccgtt tttttatctg gaggattaat gacaatcacg gacctggtac
    26161 tgattctttt catcgccgca ctcctggcct tcgcgatcta cgatcagttc atcatgcccc
    26221 gccgtaacgg ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc atcgatagcg
    26281 ttatcttcgt cggattgatt gtcattctta tctataacaa cgtcacgaat catggtgcgt
    26341 taataacgac atggttatta agcgcactgg ctctgatggg tttttatata ttctggatcc
    26401 gcgttccgaa gatcatcttt aaacaaaaag gttttttctt cgccaatgtc tggattgaat
    26461 atagccgaat caaagcgatg aacttgtcgg aagatggcgt gttggtgatg caattagaac
    26521 agcgtcggct gttaatccgc gttcgaaata tcgacgatct ggaaaaaatt tataagcttc
    26581 tcgtttcaac tcagtaagtt a
//
LOCUS       DAZFJF010000065        26365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFJF010000065 DAZFJF010000000
VERSION     DAZFJF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.065"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1632
                     /gene="yqiK"
                     /locus_tag="ACHKAU_002849"
                     /pseudo
     CDS             1..1632
                     /gene="yqiK"
                     /locus_tag="ACHKAU_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            1871..1930
                     /locus_tag="ACHKAU_002850"
     CDS             1871..1930
                     /locus_tag="ACHKAU_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5531875.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            2247..2306
                     /gene="ibsE"
                     /locus_tag="ACHKAU_002851"
     CDS             2247..2306
                     /gene="ibsE"
                     /locus_tag="ACHKAU_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO5531876.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="ACHKAU_002852"
     CDS             complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="ACHKAU_002852"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO5531877.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="ACHKAU_002853"
     CDS             complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="ACHKAU_002853"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO5531878.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(6770..8071)
                     /gene="ygiF"
                     /locus_tag="ACHKAU_002854"
     CDS             complement(6770..8071)
                     /gene="ygiF"
                     /locus_tag="ACHKAU_002854"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO5531879.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8313..8933
                     /gene="ygiM"
                     /locus_tag="ACHKAU_002855"
     CDS             8313..8933
                     /gene="ygiM"
                     /locus_tag="ACHKAU_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO5531880.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            8997..10235
                     /gene="cca"
                     /locus_tag="ACHKAU_002856"
     CDS             8997..10235
                     /gene="cca"
                     /locus_tag="ACHKAU_002856"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO5531881.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10398..11219)
                     /gene="bacA"
                     /locus_tag="ACHKAU_002857"
     CDS             complement(10398..11219)
                     /gene="bacA"
                     /locus_tag="ACHKAU_002857"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5531882.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11309..11677)
                     /gene="folB"
                     /locus_tag="ACHKAU_002858"
     CDS             complement(11309..11677)
                     /gene="folB"
                     /locus_tag="ACHKAU_002858"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO5531883.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11782..12399
                     /gene="plsY"
                     /locus_tag="ACHKAU_002859"
     CDS             11782..12399
                     /gene="plsY"
                     /locus_tag="ACHKAU_002859"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO5531884.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12412..13344)
                     /gene="ttdR"
                     /locus_tag="ACHKAU_002860"
                     /gene_synonym="dan"
     CDS             complement(12412..13344)
                     /gene="ttdR"
                     /locus_tag="ACHKAU_002860"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO5531885.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13551..14462
                     /gene="ttdA"
                     /locus_tag="ACHKAU_002861"
     CDS             13551..14462
                     /gene="ttdA"
                     /locus_tag="ACHKAU_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO5531886.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14459..15064
                     /gene="ttdB"
                     /locus_tag="ACHKAU_002862"
     CDS             14459..15064
                     /gene="ttdB"
                     /locus_tag="ACHKAU_002862"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO5531887.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15112..16575
                     /gene="ttdT"
                     /locus_tag="ACHKAU_002863"
     CDS             15112..16575
                     /gene="ttdT"
                     /locus_tag="ACHKAU_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO5531888.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16618..17631)
                     /gene="tsaD"
                     /locus_tag="ACHKAU_002864"
     CDS             complement(16618..17631)
                     /gene="tsaD"
                     /locus_tag="ACHKAU_002864"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO5531889.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17869..18084
                     /gene="rpsU"
                     /locus_tag="ACHKAU_002865"
     CDS             17869..18084
                     /gene="rpsU"
                     /locus_tag="ACHKAU_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO5531890.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18195..19940
                     /gene="dnaG"
                     /locus_tag="ACHKAU_002866"
     CDS             18195..19940
                     /gene="dnaG"
                     /locus_tag="ACHKAU_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5531891.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            20135..21976
                     /gene="rpoD"
                     /locus_tag="ACHKAU_002867"
     CDS             20135..21976
                     /gene="rpoD"
                     /locus_tag="ACHKAU_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO5531892.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22055..22561)
                     /gene="mug"
                     /locus_tag="ACHKAU_002868"
     CDS             complement(22055..22561)
                     /gene="mug"
                     /locus_tag="ACHKAU_002868"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO5531893.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22686..22761
                     /locus_tag="ACHKAU_002869"
     tRNA            22686..22761
                     /locus_tag="ACHKAU_002869"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22719..22721,aa:Ile,seq:cat)
     gene            complement(22815..23579)
                     /gene="nfeF"
                     /locus_tag="ACHKAU_002870"
                     /gene_synonym="yqjH"
     CDS             complement(22815..23579)
                     /gene="nfeF"
                     /locus_tag="ACHKAU_002870"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO5531894.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            23856..24479
                     /gene="nfeR"
                     /locus_tag="ACHKAU_002871"
                     /gene_synonym="yqjI"
     CDS             23856..24479
                     /gene="nfeR"
                     /locus_tag="ACHKAU_002871"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO5531895.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24633..26153)
                     /gene="aer"
                     /locus_tag="ACHKAU_002872"
     CDS             complement(24633..26153)
                     /gene="aer"
                     /locus_tag="ACHKAU_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO5531896.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
BASE COUNT         6325 a         7258 c         6767 g         6015 t
ORIGIN      
        1 tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgcc
       61 aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa
      121 aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc
      181 aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa
      241 gatcgtatgc gtgtcgatgt agtagtcgct ttctttgtac gggtaaaacc gtctgtagaa
      301 ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga agacttacgt
      361 atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg
      421 catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa
      481 gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt taaccagacc
      541 tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgact
      601 caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg
      661 gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg
      721 tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt
      781 attgcggctt ttgaagctga acgtcgtcgt gaagcagagt agacgcgaat tctggctgaa
      841 cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag aaaggttgag
      901 gctgaacgtg aagttcgtat taaagagatc gaacaacagc aggtcaccga aatcgccaac
      961 cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt
     1021 gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac cactcgccag
     1081 accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa
     1141 accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag
     1201 gcggcggcta tcgttgagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa
     1261 gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa
     1321 ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt tgagccgatg
     1381 aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg
     1441 ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc attatcagcc
     1501 gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt
     1561 tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc cgtcgccgaa
     1621 aacgtagaat aaattttcag ctatgaacgt ccctgtcaca aaaagccggg gattttttat
     1681 atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg cgtgaccgct
     1741 cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct catcaacaat
     1801 cggggagtca gcaaagggct gaaacgggaa agcccctccc taggaagggg ccttacagaa
     1861 ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttaatcg taagtttcgc
     1921 agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt
     1981 taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct ggcccaaaaa
     2041 gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc
     2101 gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc
     2161 ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg
     2221 aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt
     2281 tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat cctcccttac
     2341 ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc ctgctacgaa
     2401 gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgatga
     2461 tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg ccgttggccc
     2521 agacttcttt actcccggcg atctcttctg gtttatagtc gccgcccttc accagtaaat
     2581 ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac accacccagt
     2641 cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta acggggcggg
     2701 aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc agacggtcac
     2761 ccagtttgcg agcatttgcc agataggaga cgtgcccggc gtgcaggatg tcaaagacac
     2821 cgttggtcat taccaccttt tcgccgcgtt tacgcgctgc cgctacagca agcttcagtt
     2881 cctcttcggt catcacacca aagccggtat ctgcgcgccc acgcactgca ttttccagct
     2941 cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc gccgcattgg
     3001 caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa
     3061 tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt ggcatatgca
     3121 gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc actaacagag
     3181 ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct
     3241 tacatttacc gacaacagct tcaaattccg agagattcgg tgtcagcagc gtagcgccgc
     3301 ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc gctttacgcg
     3361 ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca
     3421 gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc ggatcaacac
     3481 cttcgaaacc ttcttcaaaa tccaggcgga tcagctgttg gttgcgggaa agtacccgta
     3541 atttggtgat agtcggatgc gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg
     3601 ccagagattt actcagcgca cgcgccgcat cgtcaatgcc cgtcagcccg accaggcgag
     3661 cattggcacc gagagaagcg atgttcattg ccacgttagc cgcgccgccc ggacgttctt
     3721 cgatggtatt gactttaacc acgggcaccg gcgcttccgg cgagatacgg ctggtgggac
     3781 cgtaccagta acgatccagc atcacatcac caaccaccat cactcctgca cgttcaaact
     3841 ctggcagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac
     3901 catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagtt cacgctctgc
     3961 ggtgaagcaa tcctccggca catggcccgg caattcctgt aatgccagat gatgaagttc
     4021 atcgcgcagc gtagtatacg cacgggtcag cgccatcgct tcctgctctt ccataatgtc
     4081 gttttgcgcc agtagttcca gaatgcgcac gttgtctgac cagcgcgtta acttcggttt
     4141 ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg taattccgcc
     4201 ttcatcagct ttgatatcaa agcgatcgcg atgtttattg ccgagatgag cgcgcatttt
     4261 ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tcacgcggca gcgtcataat
     4321 ctcgcgacgc accgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacccgcgc
     4381 acgcaccagc gcctgatgtt cccacgtcca ggcctcgttt ttctgataat cggcaaatgc
     4441 ttctgcggat gtcaccagca ttcccgcggc cccggacggg cgtagccgtg catccacttc
     4501 atacaaaatg ccggaagagg tacgcgtact gaacagatgc ataatgcgtt gcgccagacg
     4561 cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat ccattgggca
     4621 atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc cgcccagctt
     4681 gccgtagccg accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat ttggcttacc
     4741 gtagcgggcc accatttgaa cccacgcctg ctgaacgacg gcatctatca tagcctctgc
     4801 cagccaggtt aagtgatcgc tcactttcat caccggtagc gtaccagcga tatccgctgc
     4861 ggcgatgcgt aacagctgtg cctgtttgaa ctgacgcagc gcctcaagct gttgctcttc
     4921 gtcatcttcc ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg catcggtcgc
     4981 cgtcggctgg taaagggtgt ttggatcgag caattcatcc agcaataatg gataacgcgc
     5041 cagttggctg gcaatcatcg gcgacgcggc acacagagaa atcaaatgtt taagcgccgc
     5101 ggggaattca ctgagcaatt ctaaataggt ggtgcgggta acaatcccca ccagcaaggc
     5161 ggtaatgcgc gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac ttagcagatg
     5221 cggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atggtgcgct tatccagctc
     5281 tttgcggaaa tcggcaatca gcgttagcac ctgtttgcga tcatcctcgc taagatgcgc
     5341 cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc
     5401 cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat taaacacccg
     5461 gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt cagcaaagtc
     5521 catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgtct gggtttgttc
     5581 gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatacg ccactcgcaa
     5641 ttgttcagca tcgttttcag aaagcagatg cagcgcggca atgacgctga gcgttggcag
     5701 taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga acacctgaac
     5761 gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg tcaaaccacg
     5821 tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa tcacgctgaa
     5881 atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt tagcatagac
     5941 gccttcgcta tcgcccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc
     6001 ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc cactttcgcc
     6061 aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccaa
     6121 cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg
     6181 tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat ctgaggagaa
     6241 attcagttcc ccaccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc
     6301 ctgcgcattg cacggcgttc cccactcgcg gcagcaagcg tcatacagcc agtcacgcgc
     6361 ggcgacaatc agcgtctccg ccagatggct gagctgctgc aatatgctct cttcagtaac
     6421 caaagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga atagccgcag
     6481 ctcgcgcatt aacccggctt cgtcactcac attactgagc gcctcctgca accatgccgc
     6541 gtaatgctgc cattcgtcgg cctgcggcgg ttggctttcc agttccgtca gccactctgg
     6601 atgcacaatc acgctgtcct gcacaaaatc actaaaagta agtactgact tcgcctgtgc
     6661 gctaagtgat tcctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg
     6721 ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc
     6781 cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcaa
     6841 tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc caggcttgcg
     6901 cgacgacagg atcatagtaa cctgccagca gcagtattga gtcaatatca cgcgtcaggc
     6961 gtggcaactg atcatggtag cgatcgccta acggctggca gaaaacgctt ttcagttcag
     7021 cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctg attttaccct
     7081 gcgctttggc atctaaaaac ggctgccagg ctttgcttac caaccattcg gtcaacgcca
     7141 gctttgccat tgccgtttcg gtagagtaga ccgccgtcac ggcagaaacc gcagaagcga
     7201 tggtcgcttc gcattgagtc agcaaatcac gtaagtgagt gctcgcttta cgcggcacaa
     7261 tgccaccgaa cagcatcagt gcatggcgaa caaggccaat ggctgccagc acctgctctt
     7321 tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca
     7381 gctcgaacgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca tgcaaaatgg
     7441 tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgccgctt
     7501 tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca
     7561 ccgcgcgcgt gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt
     7621 cccccgcttt cacctccccc tgatcgagag cgatttcaat tcggctgtca tcgaccgcca
     7681 ccagccattt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat
     7741 cggcgggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg tcgagcgtcg
     7801 gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg
     7861 caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag cccatatcgt
     7921 gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat
     7981 ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa
     8041 caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta tgtcacataa
     8101 gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca
     8161 aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga
     8221 cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc
     8281 gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa
     8341 ctttactcgc acttagcgcc actgccgtct cacacgctga agaaacgcgc tatgtttctg
     8401 atgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg
     8461 ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg
     8521 tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc actgagccaa
     8581 gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc gataaactca
     8641 ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga
     8701 gcgacagcgt gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac gagctgattg
     8761 tcgcgcagaa aaaggtcgat gccgccagcg tacagttgga tgacaaacag cgcaccatca
     8821 tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg ctcggtctgg
     8881 tactgccaca cctgatccca acgcgcaaac gcaaagatcg ctggatgaac taaatcgcct
     8941 tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat gatggcgtga
     9001 agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca
     9061 gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg
     9121 taggccgcga ttttcctgtg tttctgcatc cgctaacgca tgaagagtat gcgctggcac
     9181 gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc gcaccggatg
     9241 tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg gctcaggacg
     9301 ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc
     9361 gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg gcgcgttttg
     9421 ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg ctgatgcgcg
     9481 agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg aaagagacgg
     9541 aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggtg
     9601 cacttcgcgt tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt
     9661 ggcatccgga aatcgacacg ggtattcata ccttaatgac gctctcaatg gcggcgatgc
     9721 tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt aaaggactga
     9781 cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt aagttagtgg
     9841 aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtgg
     9901 ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc atcgtcaaat
     9961 tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca
    10021 gcgaggctga cgtacgcggc agaaccggtt ttgaatcagc ggactacccg caaggccgtt
    10081 ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc gttgaagcgg
    10141 gatttaaagg tgtggagatt cgcgaggagc tgacccgacg gcggattgcg gcggtagcca
    10201 gttggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg ttaacacgaa
    10261 ccatcccctc gccccttcgg ggagagggtt agggtgaggg gaacagacca gcaccggtgc
    10321 gaacaatcac ccccaccccg gccctctccc tacagagcga gggagaaaac caggcctgct
    10381 cccgaacctt ttctcaatca aaagaacacg acgtacaccg cagccgccac aataaagcga
    10441 taaatggcga acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt tttaatcgct
    10501 atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa acatcgggat atcgccggtt
    10561 gtcaggaagc cccagctttt gtagagatcg agcgccgttg cgcccatcat catcggtacc
    10621 gccagcagga acgagaactc ggaagcagcg taacggctca cgcccatcag catcccacct
    10681 gaaatggtgg ccccggaacg ggagaaaccc ggccacagcg ccagacactg gaaacagcca
    10741 atcataaacg cctgacgata ggtcatatca tcaagacccg gcgcacgcgg ctctttcggc
    10801 ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga ccagtgcata catcacattt
    10861 atcgggttaa acaatgactt aatcgtgtcg tggaacaaca gccccagtac taccgccgga
    10921 atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt tgctttctcc ttcgtgctgc
    10981 aacgggcggc caaagtggat gccaatcagg ccaaacagac gccgccagaa catcactact
    11041 accgccagaa ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt gtcgccctcg
    11101 aaccccaaca agtgaccgac aataatcata tggcccgtgc tggataccgg cagaaattct
    11161 gtcaatcctt cgaccacacc caatattgcc gctatcagca gcgagtgcat atcgctcatc
    11221 aataaacccc taaattatta aaatgtaccg cttgtccgaa ctactgcgta tgaccaggtt
    11281 ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct
    11341 caatgattac gccaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatac
    11401 gcacccacgg cgagttaaag cgtgctaaca gcagctccgc cacctcttca gccacgcgtt
    11461 ccaccagcgc aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt
    11521 aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc catgccattt
    11581 cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg
    11641 tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct
    11701 aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga
    11761 attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac
    11821 ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg
    11881 cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga
    11941 gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg
    12001 tatgaattag gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga
    12061 cacatctggc ccgttttctt cggatttaaa ggaggaaaag gcgttgctac cgcttttggt
    12121 gccatcgcac ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc
    12181 gtgctattga gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt
    12241 tatgtatggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc
    12301 ctgctgcgtc atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg
    12361 acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac
    12421 ctgatagcct tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac
    12481 gcagacacgt aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc
    12541 gctttgtgca tactccggca atacttgcac cagtttgcca ctctccagaa acggcaacac
    12601 atcccactcg gaacgcagca taatcccttt gccctccagc gcccattgca acactatctc
    12661 gccgctattg gaggaaagat gcccgctaac cttcaccgat tttttctcct gaccgttccc
    12721 caactcccat attccgtggg tcatatcgcg ctctttggtc accagacagt catgacgact
    12781 taattcctgt aatgattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa
    12841 tattctttta tttttcgtta acagatgcgc aatataataa tcaggaattt cgtcattaat
    12901 acgaatatcc agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa
    12961 atgcacctgt aactcaggat aattgcgcat aagttcggta atggctggcg caatatggct
    13021 gcgcccaaaa ccaaaactgc aaccaatacg aatcatcccc tccgggcgcg ttttgatttg
    13081 cgtgacgtca tcgaccagtc gctgatattg ggtgaggatt tccagcgcat gttcgtaaca
    13141 acgttggcca ctttcagtca gcgccacgcc gcgagcggag cggttcagca gcgttgttgc
    13201 cagcgtattt tcaaggattt ggatccgctt ggtgacaaat gcaggcgtct gcccaagcgt
    13261 cgccgctgca gcactaaaac tcccgctgtg gacgatttct accagtacct gcaagtcttt
    13321 ggctaaaggc cagctattca gcatcgttaa ttatccgcag ttgtgataag cgcagtgtat
    13381 ttcgcaaaac attgccattc cctgctctgg cgcaatattc attcttattt tcgcgttgat
    13441 tcacgaactg ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgg
    13501 tagtttgtgg cggtgaattt ttccagacaa atacaaaaac tggagttgcc atgatgagcg
    13561 aaagtaataa gcaacaggca gtgaataagt tgacagagat tgtcgctaac tttaccgcca
    13621 tgatttctac ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgccg
    13681 aaacgtcgtc gatggggaaa attatctacc atacgatgtt cgacaacatg caaaaagcga
    13741 ttgacctgaa tcgtcctgcc tgtcaggaca ccggggagat tatgttcttc gttaaagtcg
    13801 gttcccgctt cccactgctt ggcgagctgc aaagcatact caaacaagcc gtggaagagg
    13861 caaccgtcaa agcgccacta cgtcacaatg cggtagaaat ttttgacgaa gtaaacaccg
    13921 gcaaaaatac cggtagcggc gtaccgtggg tcacctggga catcatcccc gacaatgacg
    13981 atgcggaaat cgaagtttac atggcaggcg gcggctgcac gctacctggc cgctcgaaag
    14041 tgttaatgcc gtcagaaggc tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca
    14101 ccctcgccgt aaacgcctgt ccaccggtac tggtgggcgt gggcatcgcc acctcggtgg
    14161 aaaccgccgc cgtgctctcg cgaaaagcca ttttgcgccc gattggcagc cgccatccca
    14221 atccaaaagc ggcagagctg gagctacgcc tggaagaagg actcaaccgt ctgggaattg
    14281 gcccacaagg gctgactggc aacagttcag tgatgggcgt gcatatcgaa tctgccgccc
    14341 gccatccgtc aaccatcggc gttgctgttt ctacaggctg ctgggcgcat cgtcgcggca
    14401 cactgctggt tcatgccgat ctcacctttg aaaatctgtc tcacacccgg agcgcgttat
    14461 gaaaaagatc ctgacaaccc cgatcaaagc tgaagatctg caagatattc gcgtcggcga
    14521 tgtgatctac ctgaccggta cgctggtgac ctgccgtgac gtttgtcacc gccgcttgat
    14581 cgaactgaaa cgtccgatcc cttacgatct caacggcaaa gcgattttcc acgctggccc
    14641 catcgtgcgc aaaaacggcg ataaatggga gatggtttcc gttggcccga caaccagtat
    14701 gcgtatggaa agttttgaac gtgaatttat cgagcagacc ggcgtgaaac tggtggttgg
    14761 caaaggtggt atggggccgc tgaccgaaga aggctgccag aaattcaagg cgctacatgt
    14821 gattttcccg gcaggctgcg cggtgctggc ggcaacccag gtagaagaga ttgaagaagt
    14881 gcactggaca gaactcggga tgccggagtc actgtgggtc tgccgggtca aagagttcgg
    14941 cccgctgatt gtctctattg atacccacgg caacaacctg atagccgaaa acaaaaagct
    15001 gttcgccgaa cgccgcgatc ccatcgtgga agagatctgc gagcacgtcc attacatcaa
    15061 ataaccctcc cggagaggct cacccctctc ctttttcgca ggcataacac gatgaaacct
    15121 tccactgaat ggtggcgata tcttgcgccg ctggcggtca tcgccattat tgctctactt
    15181 ccggttcccg cagggctgga gaaccatacc tggctctact ttgccgtttt tactggcgtg
    15241 atcgttggac tgatcctcga acccgtgccg ggtgccgtgg tggcgatggt gggtatctcc
    15301 atcatcgcca tactctctcc ctggctgctg ttcagccccg aacagctcgc ccagccaggc
    15361 tttaaattca ctgcaaaatc cctctcgtgg gcggtttccg gtttttctaa ttcggttatc
    15421 tggctgattt tcgccgcctt tatgtttggc acaggctatg aaaaaaccgg gcttggacgc
    15481 cgcattgcgc tgtttctggt gaaaaagatg ggacatcgca cgctgtttct cggctatgcg
    15541 gtgatgttct ccgagctgat cctcgcacct gtaacaccgt ccaactcggc gcgtggtgcg
    15601 gggattatct atcccatcat ccgtaacctg ccaccgctct atcaatcaca accaaacgac
    15661 agcagttcgc gcagcattgg ctcgtacatc atgtggatgg ggattgttgc cgactgcgtg
    15721 accagcgcca ttttcttgac ggcgatggca ccaaacttgc tgttaattgg actgatgaaa
    15781 agcgcatctc acgccacgct gagttggggc gactggttcc tcgggatgtt gccgctcagt
    15841 attttgctgg tgctgctggt tccctggctg gcttatgtgc tgtacccgcc ggtactgaag
    15901 tctggcgatc aggtgccgcg ctgggcagag acggaactgc aggcaatggg cccgctctgt
    15961 tcgcgtgaaa aacggatgct ggggctgatg gtaggcgcgc tggtgctgtg gattttcggc
    16021 ggtgattata tcgatgccgc gatggtcggt tacagcgtgg tggcactgat gctgcttctg
    16081 cgcattatca gttgggacga cattgtcagt aataaagcgg cgtggaacgt tttcttctgg
    16141 ctggcctcgc ttatcaccct cgctaccgga ctcaacaaca ctggttttat tagctggttt
    16201 ggcaaactgt tagcaggcag cttaagcggt tattcgccaa cgatggtgat ggtggcgttg
    16261 attgtggtgt tttatctgct gcgctacttt ttcgccagcg ccacggcgta tacctccgcc
    16321 ctcgcaccga tgatgattgc cgctgcgctg gcgatgccgg aaatcccgct gccggtgttc
    16381 tgcctgatgg ttggcgcggc aattggtctg gggagcattc ttacaccata cgccaccgga
    16441 cccagtccga tttactacgg tagtggttat ctgccaacgg tggattactg gcgactgggg
    16501 gcgatttttg gactgatatt cctcgtgttg ctggtgatta ccggcttgct gtggatgccc
    16561 gtggtgttgc tttaagtgag gacggtgccg gaaattgctc tccggcaccg gatagtttta
    16621 cgcagccggt aactctgcca gtggccagcg cggacgcacg ctaacgccga gatccgccgt
    16681 cgcgcctgct ttaaaccgca ccattccggc ataggcgatc atcgcgccgt tatcagtaca
    16741 aaattccgga cgcgcgtaga acacttcgcc gcggcgtttt ttcatcattt ccgccagctt
    16801 cgcccgtaac gtgcggttag cactcacgcc gcccgccatg accagtcgct taaagcccgt
    16861 ctgatccaac gctcgcttac acttaatcat caacgtatcg accaccgcat cttcaaaggc
    16921 gcgggcgata tcagcacgcg tctggtcgtc ggtgccgttg tcacgaatcg tgttcgccgc
    16981 aaaggttttc agaccagaaa agctgaaatc cagccccgga cggtcggtca tcggacgcgg
    17041 gaaaacaaag cgcccggcag taccctgagc cgccattttc gacagtaacg gtccgccagg
    17101 ataatccagc cccagcagct tcgcggtttt atcaaacgct tcaccggcgg catcatcgat
    17161 agactcgccg agcagctcgt actgaccaat gccagtcacg ctgattaact gcgtatgacc
    17221 gccggacacc agcagcgcga caaacggaaa ttccggcggg ttatcttcca gcatcggcgc
    17281 taacagatgc ccttccatat ggtgtaccgg gattgccgga acgttccagg caaacgccag
    17341 agaacgcccc acggttgcgc caaccagcag tgcgccgact aatccagggc ctgcggtata
    17401 ggccacagca tcaatgtctt ttgccgttaa accagactcc tttagcgccg cctggatcaa
    17461 cggtacggtt ttgcgcacgt gatcgcggga ggccagttca ggcacgacgc cgccgtagtc
    17521 agcgtgcaat ttcacctgac tatacaattg gttggctaac aaaccttttt catcgtcgta
    17581 aatggcgatg ccggtttcat cgcaggaagt ttcaataccc agtacacgca tgacttgttt
    17641 tacctcgctt tattaccgcg cagtgtagga ccaatgcggg ttgatgtaaa actttgttcg
    17701 cccctggaga aagcctcgtg tatactcctc acccttataa aagtcccttt caaaaaaggc
    17761 cgcggtgctt tacaaagcag cagcaattgc agtaaaattc cgcaccattt tgaaataagc
    17821 tggcgttgat gccagcggca aaccgaatta atcaaaggtg agaggcacat gccggtaatt
    17881 aaagtacgtg aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa
    17941 aaagcaggtg ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa
    18001 cgtaagcgcg ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac
    18061 gcacgccgca ctcgtctgta ctaattcccc gagagcgttg ctctccgatc agaccgagtt
    18121 gtagttgtaa ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt atgaattgct
    18181 aaaaatcggg gcctatggct ggacgaatcc cacgcgtatt cattaatgat ctgctggcac
    18241 gcactgacat cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt
    18301 tccacgcgtg ttgtccattc cacaacgaga aaaccccgtc attcaccgtt aacggtgaga
    18361 aacagtttta ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga
    18421 actacgacaa gctcgagttc gttgaaacgg tcgaagagct ggcagcaatg cacaatcttg
    18481 aagtgccatt tgaagcaggc agcggcccca gccagatcga gcgccatcag aggcaaacgc
    18541 tttatcagtt gatggacggt ctgaatacgt tttaccaaca atctttacaa caacctgttg
    18601 ccacgtctgc gcgccagtat ctggaaaaac gcggattaag ccacgaggtt atcgctcgct
    18661 ttgcgattgg ttttgcgccc cccggctggg acaacgtcct gaagcggttt ggcggcaatc
    18721 cagaaaatcg ccagtcattg attgatgcgg ggatgttggt cactaacgat cagggacgca
    18781 gttacgatcg tttccgcgag cgggtgatgt tccccattcg cgataaacgc ggtcgggtga
    18841 ttggttttgg cgggcgcgtg ctgggcaacg atacccccaa atacctgaac tcgccggaaa
    18901 cagacatttt ccataaaggc cgccagcttt acggtcttta tgaagcgcag caggataacg
    18961 ctgaacccaa tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat
    19021 acggcattaa ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat cacatacaac
    19081 tgttgttccg cgcgaccaac aatgtcattt gctgttatga cggcgaccgt gcaggccgcg
    19141 atgccgcatg gcgagcgctg gaaacggcgc tgccttacat gacagacggc cgtcagctac
    19201 gctttatgtt tttgccggat ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaag
    19261 aagcgtttga agcgcggatg gagcaggcga tgccactctc cgcatttctg tttaacagcc
    19321 tgatgccgca agttgatctg agtacccctg acgggcgcgc acgtttgagt acgctggcac
    19381 tgccattgat atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt caggaattag
    19441 gcaacaaatt aggcatactt gatgacagcc agcttgaacg attaatgcca aaagcggcag
    19501 agagcggcgt ttctcgccct gttccgcagc taaaacgcac gaccatgcgt atacttatag
    19561 ggttgctggt gcaaaatcca gaattagcga cgttggtccc gccgcttgag aatctggatg
    19621 aaaataagct ccctggactt ggcttattca gagaactggt caacacttgt ctctcccagc
    19681 caggtctgac caccgggcaa cttttagagc actatcgtgg tacaaataat gctgccaccc
    19741 ttgaaaaact gtcgatgtgg gacgatatag cagataagaa tattgctgag caaaccttca
    19801 ccgactcact caaccatatg tttgattcgc tgcttgaact gcgccaggaa gagttaatcg
    19861 ctcgtgagcg cacgcatggt ttaagcaacg aagaacgcct ggagctctgg acattaaacc
    19921 tggagctggc gaaaaagtga tttaacggct taagtgccga agagcgatcg ggaagccccc
    19981 gacagccgca ctgagaggca gcggcaaata tataagtacg ccctcgtaat tatcgttggc
    20041 ggtaaacaac cgttggattt cagcgttaac ggctgaagga catcgggtca atcgcccaac
    20101 accaacctca tgaaataagt gtggataccg tcttatggag caaaacccgc agtcacagct
    20161 gaaacttctt gtcacccgtg gtaaggagca aggctatctg acctatgccg aggtcaatga
    20221 ccatctgccg gaagatatcg tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa
    20281 cgacatgggc attcaggtga tggaagaagc accggatgcc gatgatctga tgctggctga
    20341 aaacaccgcg gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc
    20401 tgaaatcggg cgcacgactg acccggtacg catgtacatg cgtgaaatgg gcaccgttga
    20461 actgttgacc cgcgaaggcg aaattgacat cgctaagcgt attgaagacg ggatcaacca
    20521 ggttcaatgc tccgttgctg aatatccgga agcgatcacc tatctgctgg aacagtacga
    20581 tcgtgttgaa gcagaagaag cgcgtctgtc cgatctgatc accggctttg ttgacccgaa
    20641 cgcagaagaa gatctggcac ctaccgccac tcacgtcggt tctgagcttt cccaggaaga
    20701 tctggacgat gacgaagatg aagacgaaga agatggcgat gacgacagcg ccgatgatga
    20761 caacagcatc gacccggaac tggctcgcga aaaatttgcg gaactacgcg ctcagtacgt
    20821 tgtaacgcgt gacaccatca aagcgaaagg tcgcagtcac gctgccgctc aggaagagat
    20881 cctgaaactg tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct
    20941 ggtcaacagc atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa
    21001 gctctgcgtt gagcagtgca aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa
    21061 cgaaaccagc gatacctggt tcaacgcggc aattgcgatg aacaagccgt ggtcggaaaa
    21121 actgcacgat gtctctgaag aagtgcatcg cgccctgcag aaactgcagc agattgaaga
    21181 agaaaccggc ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt ccatcggtga
    21241 agcgaaagcc cgccgtgcga agaaagagat ggttgaagcg aacttacgtc tggttatttc
    21301 tatcgccaag aaatacacca accgtggctt gcagttcctt gacctgattc aggaaggcaa
    21361 catcggtctg atgaaagcgg ttgataaatt cgaataccgc cgtggttaca agttctccac
    21421 ctacgcaacc tggtggatcc gtcaggcgat cacccgttct atcgcggatc aggcgcgcac
    21481 catccgtatt ccggtgcata tgattgagac tatcaacaaa ctcaaccgta tttctcgcca
    21541 gatgctgcaa gagatgggcc gtgaaccgac gccggaagaa ctggctgaac gtatgctgat
    21601 gccggaagac aagatccgca aagtgctgaa gatcgccaaa gagccaatct ccatggaaac
    21661 gccgatcggt gatgatgaag attcgcatct gggggatttc atcgaggata ccaccctcga
    21721 gctgccgctg gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc
    21781 tggcctgacc gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac
    21841 cgaccacacg ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca
    21901 gatcgaagcg aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag tgttgcgtag
    21961 cttcctggac gattaatcgg taggccggat caggcgttac gccgcacccg gcactagtcc
    22021 ctttgcacaa acgccacctt ttcggtggcg ttttttatcg cccacgcact accagcgcct
    22081 ggtccagctc gcgatatgct tcaaccagtt tctctagtga aacgcgactt aaaccgctgg
    22141 gatttggcag cacccaaatc tgcgtcgaac caatggtgag cgtttgtttc ccccactgtg
    22201 caccgcgctg gctgaatccc tgttcatatg cttgtttgcc cagaatcgcc aacgcctgcg
    22261 gctgataatc ttcaattttt tcaatcagct tacgcccacc tgcgtgcagc tcctgctttg
    22321 aaacttcatt ggcttgcacc gttggacggt ctaccagttt ggtgacgcca caacgataat
    22381 ccagcagatg ctgtgcctcc tgcggcttca actgacggtc ggtaaacccg gcctgatata
    22441 tcaccttcca gaagcgattt gctggatgag caaagggaaa accagtcccg gcggatgaaa
    22501 gcccagggtt gataccgcaa aacacgaccc gtaaccctgg agccaaaata tcctcaacca
    22561 tatttactcc tttctaacat caccaggaaa agtataacgg attgaaaata cattgtttat
    22621 aaaaacagca ggcgcacggt aatggctgga ttgcgacacg gagttacttt ataatccgct
    22681 accatggccc cttagctcag tggttagagc aggcgactca taatcgcttg gtcgctggtt
    22741 caagtccagc aggggccacc agatatagca aaggctgacg agaaatcgtc agcctttttc
    22801 tttttatata tcagttactt tgcgtgccag taagccgctg cacgtacccg ctgtgggtca
    22861 tactgttccg cttcaaagcg gcggcttaaa ttcttaacga ctttaccttc gccggttatc
    22921 cagatgaagt aatcatcggc agggatttgc atctgcgcca gacgcgcatc taccgcctgc
    22981 tcatcatgtg ccagccattc gatattaaaa ccatcaaggt gcgcgagata atcctgacag
    23041 gcgttatccc gcacgctaac cagcgcacta acttgcggtt taacggcaag tttgctcaac
    23101 gtttccaggc ggcggcgcag tgcgggcatt ccggattcat cgcagacata cagctgatac
    23161 gcgtaatctt ccggcaccac cagcgaaccg cgcggacctg ccaccgtaag tttatcgccc
    23221 ggttgcgcct gcatcgccca gccgctggcg accccaccgt cgtgaataaa gaaatcgatc
    23281 gccagttcat ggcgtagttc gtcatacagc ggcgtatagt cacgcgacag tgggcgtggt
    23341 ccttccggcc agacgatgcc ctcttccgtt accgttggcg gcacaaagtg agcgtcaggt
    23401 tgaggaaaga agagtttgct gtgatcgtca aagccgcgcg atgtaaaacc gtccagcgcc
    23461 tcgccgccga ggacaatgcg ctgaaaaccg gcgctgatgc gctcaacgcg taacacagtc
    23521 agttcacgga agcgcagatc attgcgaacg cgctgcgggt agcgggggga gttattcatt
    23581 gttatcgcct tcgtgatagt aatcagatat atctaaatga ttttcgtaaa tgataatgat
    23641 tgttaatcat gataaatgca agcgatttgt agaactgata tatctgaagt atgtgaaggc
    23701 attcagtgcc atagcaatgc tgccggatgc ggcgtgaacg tcttatccgg cctggatgtg
    23761 aggtgcttcc acgtttaaaa atcatttttg tacttgcagt aatgctcaca tcagatataa
    23821 attagatata tctaatcaag caaaaggagg ctgatatgag ccatcatcac gaagggtgtt
    23881 gtaaacatga aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg
    23941 agcactgcgg acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc
    24001 gcggcggcgg tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg
    24061 atattctctc gcgcgatgac agccacggtt acgaattgat taaagcgatt gagaacctaa
    24121 cccaggggaa ttacacccca agcccgggcg tcatctaccc aacgctggat tttctgcagg
    24181 agcagtcgct gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg
    24241 aacaaggcgc gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca
    24301 tcaaagcgcg ttgcgttggc gcggcgctgc gccagaaccc gcaaatgaag cgggcgctgg
    24361 ataattttaa agcggtgctg gatttacacg tcaaccagag cgatatcagt gatgcacaaa
    24421 taaaaaagat cattgcggtg atcgaccgcg ccgcttttga tattacgcaa ctggattaat
    24481 cgccgcatcc gccagtggcg cggtgcaatt gccggatgcg acgcttgacg cgccttatcc
    24541 ggcctacacc cgctacacac cccgcaggcc tgataagatg cgccagcatc gcatcaggca
    24601 ttgtgctcca accgccggat ccggcatacc gattaatgca gcaccgtcac cgcgtcttcc
    24661 agtcggctgg cgcggtgttt caccatcgcc gacacctgcg cactctcttc caccagctcg
    24721 gcatttttct gggtgatcag gttaagctca tccactgcac gggtcaggct ggaaagccca
    24781 tcggcctgtt ccagcgttga atggctaatc tgggcgatca actgggtgac gtttttcacc
    24841 tgtgccacaa tatcttccat cgtccgtccg gcggcgtgta cctgctgcga accggattgc
    24901 accttatcag cactggcatc aatcagcttg cgaatatcgt tggcagcatt agcgctgcgg
    24961 ctggctaaat gacgcacttc ccctgccacc actgcaaaac ctttgccctg ttcgccggca
    25021 cgcgccgctt ccaccgccgc attcagggcc agaatattgg tctgaaacgc aatatcgtta
    25081 atcagcgaag taatggtgcc aatgcgctgg gtactgtcgg cgatatcgtc cattgtcttg
    25141 atcaccgtgg tcatcgcctc cccaccctgc actgccgcat tactggcggt gatagaaagt
    25201 ttatcggcag ccgacgccgt ggcagagttc tgtttcaccg acgccgccat ttggttcatg
    25261 gtcgccaccg tttgctgaac gttatcaact gtctgctggg tatgttcgtt cagttcatcg
    25321 gtgcctttcg ccagcgtctc actgccattt ctgacactgg acacctggct tgagacatcg
    25381 ttaattagcc aacggcacat caggccaagc tgccctaccg cgcgtaatgt cagccccagc
    25441 tcatcgctgc gattcaggtg ctcaacacta ttacgctctc cggtcgccac cttcagcgcc
    25501 tgacgggcga cattttctat cgggcgcaca atctgccatt caaaacaagc gcttgccaac
    25561 aataccacta acacacagag gaaatacgtc accaccgggg cagcaacaaa ccaaagcatg
    25621 gccgccagca agataaacat cagggtcatc actccacgcg cccgccagcg aagcggtaat
    25681 gaaggcagtt tacccagcca gcctttacgc accaccaggc ctttatgaat acgcttactg
    25741 gtacgtccgg cgttcagcgc tttgtacagc ggctccaccg ccgcaatctc ttcatccgtc
    25801 gcccgggtac gaatcgacat atagccactg atttttccct cgcgcaccat cggtaccgca
    25861 ttggcccgca cccaataatg gtcgccattt ttgcggcgat ttttcacgat gccgctccag
    25921 ggctcccctt ttttcagggt gaaccacata tccgcaaacg ccgcttttgg catatccggg
    25981 tgacgcacca tgttgtgcgg ctgcccttgt aactcttgca aggtaaagcc gctcacctgc
    26041 acaaaagtgt cattagcatg agtgatatag ctttgcagat cggtagtgga catcagagtg
    26101 gtatcgtccg ccagcggggt attttgctgg gtgacatacg gatgagaaga catgttcgcg
    26161 tcctgtgcag gttatatggt tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg
    26221 ttatctttaa ccgattaatt tgatttagat cgcaatttgc gattaaagca caaatctaat
    26281 tctttgattt aaaatacttt cactctgtta ctatacgaaa acgttaatta tcttgcccaa
    26341 aaatcaggca attattgccc tgaaa
//
LOCUS       DAZFJF010000066        26359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFJF010000066 DAZFJF010000000
VERSION     DAZFJF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.066"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKAU_002873"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKAU_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO5531897.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHKAU_002874"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHKAU_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO5531898.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHKAU_002875"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHKAU_002875"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO5531899.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHKAU_002876"
     CDS             complement(1154..1621)
                     /locus_tag="ACHKAU_002876"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO5531900.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHKAU_002877"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHKAU_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO5531901.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHKAU_002878"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHKAU_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO5531902.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHKAU_002879"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHKAU_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO5531903.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHKAU_002880"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHKAU_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO5531904.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHKAU_002881"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHKAU_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO5531905.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHKAU_002882"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHKAU_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO5531906.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHKAU_002883"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHKAU_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO5531907.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHKAU_002884"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHKAU_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO5531908.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHKAU_002885"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHKAU_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO5531909.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHKAU_002886"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHKAU_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO5531910.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHKAU_002887"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHKAU_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO5531911.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHKAU_002888"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHKAU_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO5531912.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHKAU_002889"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHKAU_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO5531913.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHKAU_002890"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHKAU_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO5531914.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHKAU_002891"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHKAU_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO5531915.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHKAU_002892"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHKAU_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO5531916.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHKAU_002893"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHKAU_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO5531917.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHKAU_002894"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHKAU_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO5531918.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHKAU_002895"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHKAU_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO5531919.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHKAU_002896"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHKAU_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO5531920.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHKAU_002897"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHKAU_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO5531921.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHKAU_002898"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHKAU_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO5531922.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHKAU_002899"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHKAU_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO5531923.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHKAU_002900"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHKAU_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO5531924.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHKAU_002901"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHKAU_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO5531925.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHKAU_002902"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHKAU_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO5531926.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHKAU_002903"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHKAU_002903"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO5531927.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHKAU_002904"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHKAU_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO5531928.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHKAU_002905"
     CDS             15761..16129
                     /locus_tag="ACHKAU_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO5531929.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHKAU_002906"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHKAU_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO5531930.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHKAU_002907"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHKAU_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO5531931.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHKAU_002908"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHKAU_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO5531932.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHKAU_002909"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHKAU_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO5531933.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHKAU_002910"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHKAU_002910"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO5531934.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHKAU_002911"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHKAU_002911"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO5531935.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHKAU_002912"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHKAU_002912"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO5531936.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22834
                     /gene="dprA"
                     /locus_tag="ACHKAU_002913"
     CDS             21710..22834
                     /gene="dprA"
                     /locus_tag="ACHKAU_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO5531937.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22806..23279
                     /gene="smg"
                     /locus_tag="ACHKAU_002914"
     CDS             22806..23279
                     /gene="smg"
                     /locus_tag="ACHKAU_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO5531938.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23308..23850
                     /locus_tag="ACHKAU_002915"
     CDS             23308..23850
                     /locus_tag="ACHKAU_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO5531939.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHKAU_002916"
     CDS             23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHKAU_002916"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO5531940.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24432..25250
                     /gene="aroE"
                     /locus_tag="ACHKAU_002917"
     CDS             24432..25250
                     /gene="aroE"
                     /locus_tag="ACHKAU_002917"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO5531941.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25247..25504
                     /locus_tag="ACHKAU_002918"
     CDS             25247..25504
                     /locus_tag="ACHKAU_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO5531942.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25480..26034)
                     /locus_tag="ACHKAU_002919"
     CDS             complement(25480..26034)
                     /locus_tag="ACHKAU_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO5531943.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6765 a         6267 c         6839 g         6488 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgctagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 aggtccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg ttagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttctgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggca atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaaatct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtagttcacc caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc
    11521 agccagtaca cccgttacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccagg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagattactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaacat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacaac gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc ccacttatct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgt catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtag
    15841 cggcgaacca ttgatactgg atgatgattc tcatgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcagcgga gggaagcaat
    15961 tcagcttctg gatatcctga gaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acctgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggcgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ttcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactgaca ggattacact cgcgccgtaa aataaccaac tggagttttt
    16621 atgagccgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggtcttct gctttcaggc gcttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgc cagtaatact tcttctttag ttggtgcagg tgcggcagct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaaatc gatcccgcca
    17101 attaataagc ccagaggagg catgatgata tcggcaacca gtgaagagac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggttgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacactc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcacgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttccacaatg gtcgaacctt gtggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcgttac caatcagcgg gccgccataa taggctttaa ccacagcgag gctgacttta
    18301 ccctcagcga agttcaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtgatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg taccaggcag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
    18901 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgctttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc cagaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccaa caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttagcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcataaaacc cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgtta atcagcccct taagttgcgg gcgcttaatt
    19741 gcgacagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca caccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgtgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg atttgttcaa cggcctgtgc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaatgcccg gtaagaccgg gcttagaaga
    20101 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga attcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc
    20341 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa
    20401 agcgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgctagtt gtttcaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata aagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 actgctttag gcagaattaa gccataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggctttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aaccgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatctagttt ttcaactttc tgacgaatac
    21121 gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagcccct ctgcttccag ttcaaatggt ttaccgtcgc
    21241 ggtcaagggc gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc gtacatcgtc tcgaacatat
    21481 catcgacgat acgctgtatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agaaatttgg ctgcgtttaa tgagtatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcaaat tgatgcggtt ggattgcagc aaacagggct
    21841 tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact
    21901 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
    21961 tcaacttctg gcgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc
    22021 gctgcattca tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg
    22081 atggggacgg ttattttgcg aaactctggc gacgcgtgga gtgacaatta cgagtggact
    22141 ggcgcgtgga atcgatggtg tggcgcataa agcggcctta caggtaaatg gcgtcagcat
    22201 tgctgtattg gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc
    22261 cagtctgctt gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct
    22321 tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt
    22381 ggtggaagcg gctttgcgca gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg
    22441 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
    22501 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
    22561 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
    22621 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
    22681 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
    22741 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
    22801 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt
    22861 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag
    22921 atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag
    22981 aaccgatgca actggcctct gatccactct ccatgcgtat ttatacccct gaggagtgtg
    23041 aaagactgga tgccagctgt cgtggttttc tgcttttcct tgagcaaatt caggtgctta
    23101 accttgaaac ccgtgaaatg gtgatagagc gagtgctcgc gctggatacc gcagagttcg
    23161 acctggaaga cctgaaatgg gtaatcctga tggtgttgtt caatattccg ggctgcgaaa
    23221 atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat
    23281 catttcttta attcagcata agttgttatg gcgaaatcag cactgttcac ggtgcgtaat
    23341 aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa acacggtccg
    23401 tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg
    23461 gatggacata ttgtcaaagt tctggagggg caggtttgcc ttgcatgtgg cgcaaatctg
    23521 gtattacgcc agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa
    23581 cataccgaac ttatcgataa accggacgaa acagcaatta catgccccca atgccggacg
    23641 ggccatctgg tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac
    23701 ccggagtgtc aatttgccat taacttcaaa cccatagctg gagaatgccc tgagtgtcat
    23761 tatccactac tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa
    23821 caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc
    23881 tatcgcagct gcgatagatg tcctcaatga agaacgtgtc atcgcctatc caacggaagc
    23941 cgttttcggt gttgggtgcg atcctgatag cgaaacagca gtgatgagac tgttggagtt
    24001 aaaacagcgt ccggttgata aggggctgat tttaatcgcg gcaaattacg agcagcttaa
    24061 accctatatt gatgacacca tgctgactga tgcgcagcgt gaaaccattt tttcccgctg
    24121 gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg
    24181 ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca
    24241 ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg
    24301 aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac
    24361 gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca
    24421 ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc
    24481 cattcattca tcagcaattt gctcagcaac tgaatattga acatccctat gggcgcgtgt
    24541 tggcacccat caatgatttc atcaacacac tgaacgcttt ctttcgtgct ggtggtaaag
    24601 gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc cagagcggat gagcttactg
    24661 aacgggcagc gttggctggt gctgttaata ccctcaagcg gttagaagat ggacgcctgc
    24721 tgggtgacaa taccgatggt ataggtttgt taagcgatct ggaacgtctg tcttttatcc
    24781 gtcctggttt acgtattctg ctcatcggcg ctggtggagc atctcgcggc gtactactgc
    24841 cacttctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag
    24901 agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg
    24961 aaggtcatga gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc
    25021 cggcgatccc gtcatcgctc attcatccag gcatttattg ctatgacatg ttctatcaga
    25081 aaggaaaaac tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg
    25141 gtttaggaat gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc
    25201 ctgacgtaga accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat
    25261 ccagtttccg gacagagaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct
    25321 cgtgaatggc atgcaactga catgtgcgat ctctggcgag agtctggcgt atcgttttac
    25381 tggagatacg ccagaacagt ggttagcgag ttttcgtcag catcgctggg atctggaaga
    25441 agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc
    25501 ctgatccaga tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc
    25561 cttctcttca tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc
    25621 gctctccagc cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc
    25681 tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt
    25741 gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt
    25801 gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt
    25861 ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat
    25921 ccccacatca tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac
    25981 gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac
    26041 ctcagcaata aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt
    26101 tagtatgcaa aaaagcaccg ttttgtgtgc gaatgcagca aaaagggtga aaaaacaaca
    26161 aacagaaaaa aagatcaaaa aagtacttgt gcaaaaaatt gggatcccta taatgcgcct
    26221 ccgttgagac gacaacgtga aacacttcac aagatggtcg taacaacgaa gagaaaaaat
    26281 cctgaaatta agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt
    26341 gagctgaaag ccgcgtcgc
//
LOCUS       DAZFJF010000067        26276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFJF010000067 DAZFJF010000000
VERSION     DAZFJF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.067"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..627)
                     /locus_tag="ACHKAU_002920"
     CDS             complement(<1..627)
                     /locus_tag="ACHKAU_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO5531944.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRAS"
     gene            943..1707
                     /gene="ydiQ"
                     /locus_tag="ACHKAU_002921"
     CDS             943..1707
                     /gene="ydiQ"
                     /locus_tag="ACHKAU_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO5531945.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            1727..2665
                     /gene="ydiR"
                     /locus_tag="ACHKAU_002922"
     CDS             1727..2665
                     /gene="ydiR"
                     /locus_tag="ACHKAU_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO5531946.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            2721..4010
                     /gene="ydiS"
                     /locus_tag="ACHKAU_002923"
     CDS             2721..4010
                     /gene="ydiS"
                     /locus_tag="ACHKAU_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5531947.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            4007..4300
                     /gene="ydiT"
                     /locus_tag="ACHKAU_002924"
     CDS             4007..4300
                     /gene="ydiT"
                     /locus_tag="ACHKAU_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5531948.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            4357..6003
                     /gene="fadK"
                     /locus_tag="ACHKAU_002925"
     CDS             4357..6003
                     /gene="fadK"
                     /locus_tag="ACHKAU_002925"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO5531949.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6060..8438)
                     /gene="ppsA"
                     /locus_tag="ACHKAU_002926"
     CDS             complement(6060..8438)
                     /gene="ppsA"
                     /locus_tag="ACHKAU_002926"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO5531950.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            8771..9604
                     /gene="ppsR"
                     /locus_tag="ACHKAU_002927"
     CDS             8771..9604
                     /gene="ppsR"
                     /locus_tag="ACHKAU_002927"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO5531951.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
     gene            9761..10807
                     /gene="aroH"
                     /locus_tag="ACHKAU_002928"
     CDS             9761..10807
                     /gene="aroH"
                     /locus_tag="ACHKAU_002928"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO5531952.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            10939..11130
                     /gene="hemP"
                     /locus_tag="ACHKAU_002929"
     CDS             10939..11130
                     /gene="hemP"
                     /locus_tag="ACHKAU_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO5531953.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(11134..12570)
                     /gene="selO"
                     /locus_tag="ACHKAU_002930"
     CDS             complement(11134..12570)
                     /gene="selO"
                     /locus_tag="ACHKAU_002930"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO5531954.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(12633..13346)
                     /gene="ydiV"
                     /locus_tag="ACHKAU_002931"
     CDS             complement(12633..13346)
                     /gene="ydiV"
                     /locus_tag="ACHKAU_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO5531955.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(13593..14057)
                     /gene="nlpC"
                     /locus_tag="ACHKAU_002932"
     CDS             complement(13593..14057)
                     /gene="nlpC"
                     /locus_tag="ACHKAU_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5531956.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(14135..14884)
                     /gene="btuD"
                     /locus_tag="ACHKAU_002933"
     CDS             complement(14135..14884)
                     /gene="btuD"
                     /locus_tag="ACHKAU_002933"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO5531957.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(14884..15435)
                     /gene="btuE"
                     /locus_tag="ACHKAU_002934"
     CDS             complement(14884..15435)
                     /gene="btuE"
                     /locus_tag="ACHKAU_002934"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO5531958.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(15498..16478)
                     /gene="btuC"
                     /locus_tag="ACHKAU_002935"
     CDS             complement(15498..16478)
                     /gene="btuC"
                     /locus_tag="ACHKAU_002935"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO5531959.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            complement(16579..16878)
                     /gene="ihfA"
                     /locus_tag="ACHKAU_002936"
     CDS             complement(16579..16878)
                     /gene="ihfA"
                     /locus_tag="ACHKAU_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO5531960.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(16883..19270)
                     /gene="pheT"
                     /locus_tag="ACHKAU_002937"
     CDS             complement(16883..19270)
                     /gene="pheT"
                     /locus_tag="ACHKAU_002937"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO5531961.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(19285..20268)
                     /gene="pheS"
                     /locus_tag="ACHKAU_002938"
     CDS             complement(19285..20268)
                     /gene="pheS"
                     /locus_tag="ACHKAU_002938"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO5531962.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(20478..20602)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(20552..20596)
                     /gene="pheM"
                     /locus_tag="ACHKAU_002939"
     CDS             complement(20552..20596)
                     /gene="pheM"
                     /locus_tag="ACHKAU_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO5531963.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(20719..21075)
                     /gene="rplT"
                     /locus_tag="ACHKAU_002940"
     CDS             complement(20719..21075)
                     /gene="rplT"
                     /locus_tag="ACHKAU_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO5531964.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(21128..21325)
                     /gene="rpmI"
                     /locus_tag="ACHKAU_002941"
     CDS             complement(21128..21325)
                     /gene="rpmI"
                     /locus_tag="ACHKAU_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO5531965.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(21422..21964)
                     /gene="infC"
                     /locus_tag="ACHKAU_002942"
     CDS             complement(21422..21964)
                     /gene="infC"
                     /locus_tag="ACHKAU_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO5531966.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(21968..23896)
                     /gene="thrS"
                     /locus_tag="ACHKAU_002943"
     CDS             complement(21968..23896)
                     /gene="thrS"
                     /locus_tag="ACHKAU_002943"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO5531967.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            24420..26276
                     /gene="espL1"
                     /locus_tag="ACHKAU_002944"
                     /pseudo
     CDS             24420..26276
                     /gene="espL1"
                     /locus_tag="ACHKAU_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
BASE COUNT         6648 a         6818 c         6241 g         6569 t
ORIGIN      
        1 gctggcacga aattttttcg ataactgttc taaagtgatt ttttcgcgat aattattatt
       61 cagatagaaa agaatatctt ttatcagaac atctttttta atatacccct gctccgaacg
      121 ataagcattt ttaaaatttt cgtagtaaag aatggctaac gtataggcaa atgcatccca
      181 taacgaagat gtttggctat ttttactttg cggcaaaatc acacttagct cattaaataa
      241 ggtttttatg acttcttttc cctgccccgc ggcaattacc ggacaagaat gcggttgcag
      301 tagctgattt tcctcagccc cctgaaactg aaagccgtac agcgcacagg tacacgtcgt
      361 tgccggatcg ttaacgtcag aggccaccgc atgtagcctg ccgcgttcta tcaccacaat
      421 gtcatccgca tgtgcgacat ataacgaaga atcgatggta aaccttgcaa cccctttctt
      481 gacgtaaatc aactcggttt cattgtcatg aacgtgatgt ccggactccc attttggatc
      541 atcgctaaat gcaaaacgtg aaagccgtgg cgttttaccg gcgacaaaca gcgtttcact
      601 ggcgttatca aaacagcgtt gatacatgac aacctcccta ttccatgagc aagcaaaaac
      661 aatatatgcc ggataaaata cggcgtgtat tgcggttata caaccgcgtt tagcgagagc
      721 taaacaagat tatttacact gaatgcaaga ttgtacggtc atggaataac tatattgccg
      781 caaattgcac ttttgtttta gcgatcgcat tttttttgca agattgttgg caaggaaaac
      841 agcttgctcc gtcgaaaacc ccgcaccgct atcgcacact attttcaggc catttttacc
      901 ttccatcgga gatggttccg tatgcgactc acaggagaaa tcatgaaaat aataacctgc
      961 tttaagctgg tgcctgaaga acaggacatt gttgtcactc cagaatacac cctgaatttc
     1021 gacaatgccg acgccaaaat cagccagttc gatctcaatg ccattgaagc tgcaagccag
     1081 ctcgcaacag atgacgatga gatagccgcg ctgaccgttg gcggctcatt gttgcagaac
     1141 tcgaaagtgc gcaaagacgt gctatcccgc gggccgcaca gcctgtattt ggtgcaggat
     1201 gcgcaacttg aacatgcact gcctctcgat accgcaaaag cgctggcggc agcaattgaa
     1261 aagatcggct tcgatttact gatctttggt gaaggttccg gcgaccttta tgcccagcag
     1321 gttggcttgc tggtcggaga aattctgcaa cttccggtga ttaatgcagt gagtgctatt
     1381 cagcgtcagg gcaatacact ggtgattgaa cgcacgcttg aagatgatgt tgaagttatt
     1441 gaactctctg ttccagccgt gctctgcgtc acctccgata ttaacgtgcc acgcattcct
     1501 tcgatgaaag ccattctcgg cgcgggtaaa aaaccggtaa atcagtggca ggcaagtgat
     1561 attgactgga gccagagcgc gccacttgct gaactggtcg gcattcgcgt accgccgcaa
     1621 acagaacgta agcacatcat tatcgataac gattcgccgg aggccattgc cgagctggcg
     1681 gaacatctga agaaagccct gaactgaagc ctacggagaa gaaacgatga gtcaattaaa
     1741 cagcgtctgg gtctttagcg ataatcctga acgttatgct gaactgtttg gcggcgctca
     1801 gcaatggggc caacaggtgt atgccattgt acaaaatacc gaccaggcgc aggcagttat
     1861 gccttatggt ccaaaatgta tttatgttct tgagcaaaac gacgcgctgc aacgcactga
     1921 aaattacgcc gaaagcattg ctgccctgct gaaagataaa caccccgcta tgttgctgtt
     1981 ggccgcgacg aaacgtggta aagctctggc agcacggtta agtgtgcaac tgaatgcggc
     2041 gctggtgaac gatgccacgg cggtggatat tgtcgatggt cacatttgcg ccgaacaccg
     2101 gatgtatggc gggttagcgt tcgctcagga aaagatcaac agcccgctgg cgatcattac
     2161 ccttgcaccc ggtgttcagg aaccgtgcac cagtgatacc tctcatcagt gcccgacaga
     2221 aacggtacct tatgttgctc cgcgtcatga aattctctgt cgcgaacgcc gtgcgaaagc
     2281 cgcaagcagc gtggacctga gcaaagcaaa acgtgtggtt ggcgtcggtc gtggtctggc
     2341 ggcgcaggat gacctgaaaa tggtccacga actggcggcg gtgctgaatg ctgaagtcgg
     2401 ctgttcacgt ccaattgccg aaggcgagaa ctggatggag cgtgaacgtt atatcggtgt
     2461 ctccggcgtg ttgctgaaat ccgatctcta cctgacgctg gggatctccg ggcagatcca
     2521 gcatatggtt ggcggcaacg gcgcaaaagt gattgtcgcc atcaataaag ataaaaatgc
     2581 gccaatcttc aattatgccg actacggtct ggtgggcgat atctacaaag tcgtccctgc
     2641 cctgattagc cagttgagcc gctaattccc ctttcaacca catctcccgc tgtaaacgca
     2701 gcgggaaggg agcataacgc atgtcggaag acaaatttga tgccattgtg gtcggtgcgg
     2761 gcgttgctgg tagcgttgcc gcactggtca tggcgcgagc cgggctggat gtcctggtga
     2821 tagaacgcgg cgacagtgcc ggatgtaaaa acatgaccgg cgggcgtctt tatgcccaca
     2881 cacttgaagc aatcattcca ggctttgcag tatcagcgcc ggtagaacgc aaggtcacac
     2941 gcgagaaaat ctccttctta accgaagaaa gcgccgttac cctcgatttt caccgcgagc
     3001 aaccagatgt tccgcaacac gcatcttata ccgtattgcg taatcgtctg gacccgtggt
     3061 tgatggaaca agccgagcag gcgggcgcgc agtttatccc aggcgttcgc gtcgatgcgc
     3121 tggttcgtga aggaaacaag gtcactggcg tgcaggctgg ggatgatatt ctcgaagcga
     3181 atgtggtgat tctggctgat ggcgttaact cgatgcttgg ccgctcgctg ggaatggttc
     3241 ccgcttccga tccgcatcat tacgctgttg gtgttaaaga ggttattggc ctcacaccag
     3301 aacagatcaa cgatcgcttt aatattacgg gcgaggaagg tgccgcctgg ctgtttgccg
     3361 gttccccttc tgacggcctg atgggcgggg gatttctcta taccaacaag gattccatat
     3421 ccttggggct ggtttgtgga ttgggtgata tcgcccatgc gcaaaaaagc gtgccgcaaa
     3481 tgctggaaga ttttaaacaa caccccgcca ttcgcccact aattagcggc ggcaaactgc
     3541 ttgaatattc cgcgcatatg gtgccagaag gcggtctggc aatggtgccg cagatggtta
     3601 acgatggcgt gatgatcgtt ggtgacgccg caggcttctg cctgaatttg ggttttacag
     3661 ttcgtggcat ggatttagcc attgcatcgg ctcaggctgc cgccacaacg gtgatcgccg
     3721 ccaaagaacg cgaggatttc tccgccagca gtctggcgca atacaaacgt gagctggaac
     3781 aaagctgcat catgcgcgat atgcagcatt tccgcaagat cccggcgctg atggaaaacc
     3841 cgcgcctgtt tagccaatac ccacgaatgg tcgccgacat catgaacgag atgttcacca
     3901 ttgacggcaa accaaaccag ccggtacgca aaatgatcat gggacatgcg aagaaaattg
     3961 ggctgatcaa cttgctgaaa gatggcatta agggagcaac cgcgctatga gccagaacgc
     4021 tacggttaac gtcgacatca aattaggcgt caataaattc catgttgatg agggccaccc
     4081 gcatatcatt ctggcggcaa atcccgatat caatgaattc cgtaaattaa tgaaagcctg
     4141 ccctgccgga ctttataagc aggatgacgc cggcaacatt cattttgatt ctgccggttg
     4201 tctggagtgc ggcacctgtc gggtgctgtg cggtaacact attctcgaac agtggcaata
     4261 tcccgcaggc accttcggta ttgattttcg ctacggctaa tcatgcatcc cacaggcccg
     4321 catctcgggc ctgatgttct gtttcgagag tccaacatga aagtgacatt aacgttcaac
     4381 gaacaacgtc gtgcggcgta tcgtcagcaa gggctatggg gcgatgcttc gctggccgat
     4441 tactggcagc agaccgctcg tgcgatgcca gacaaaattg ccgtggtcga taatcatggt
     4501 gcatcgtaca cctatagcgc gctcgatcac gccgcgagct gtctggcaaa ctggatgtta
     4561 gcgaagggta ttgaatcagg cgatcgcatc gcatttcaac tgcctggctg gtgtgaattt
     4621 accgttatct atcttgcctg cctgaaaatc ggtgcggttt ccgtgccgct gttgccttcc
     4681 tggcgggaag cagaactggt gtgggtgctc aataagtgtc aggcaaaaat gttctttgca
     4741 ccgacgttgt ttaaacaaac gcgcccggta gatttaatcc tgccgctgca aaatcagctt
     4801 ccacaactac aacaaattgt cggcgtggac aaactggctc ccgccacctc ttccctctca
     4861 ttaagtcaga ttatcgccga caatacccca ctgaccacgg cgataacgac ccacggcgat
     4921 gaattagctg cggtgctgtt tacctccgga accgagggtc tgccaaaggg cgtgatgcta
     4981 acgcataaca atattctcgc cagtgagcgg gcttattgcg cgcggctgaa tctgacctgg
     5041 caggatgtct ttatgatgcc tgcgccactc ggccacgcaa cgggctttct gcatggcgta
     5101 acggcaccat tcttaattgg cgctcgcagc gtgttgttag atattttcac tcctgatgcg
     5161 tgtctcgcgc tgcttgagca gcagcgttgc acctgtatgc taggcgcaac gccgtttgtc
     5221 tatgatcttt tgaatgtact agagaaacaa cccgcggacc tgtcagcgct gcgtttcttt
     5281 ctttgcggcg gaaccacaat ccccaaaaaa gtggcgcgtg aatgccagca gctcggcatt
     5341 aaattattaa gtgtttatgg ttccacagaa agttcgccgc atgcggtggt gaatctcgat
     5401 gatcctttgt cgcgctttat gcacaccgat ggttacgctg ccgcaggtgt agagattaaa
     5461 gtggtcgatg acgcacgcaa gaccttaccg ccaggttgcg aaggtgaaga agcctcgcgt
     5521 ggccccaatg tgtttatggg atattttgat gaacctgaat taaccgcccg tgcgctggat
     5581 gaagaaggct ggtattacag cggcgatctc tgccgtatgg atgaggctgg ctatataaaa
     5641 attaccggac gcaaaaaaga tattattgtc cgcggcggcg aaaatattag cagccgtgaa
     5701 gtggaagata ttttattgca gcatcctaaa attcacgatg cctgtgtggt tgcaatgccc
     5761 gatgaacgtt taggtgaacg atcatgcgct tatgtcgtgc tgaaagcgcc gcatcattca
     5821 ttatcgctgg aagaggtggt ggcttttttt agccgtaaac gggtcgcaaa atataaatat
     5881 cctgaacata tcgtggtaat cgaaaaacta ccgcgcactg cctcaggtaa aatacaaaag
     5941 tttttgttaa gaaaagatat tatgcggcga ttaacgcagg atgtctgtga agagattgaa
     6001 taagttttat cttcggggat cacataaacc cggcgactga acgccgccgg ggatttattt
     6061 tatttcttca gttcagccag gcttaaccag gtttgcacca cggtgtccgg gttcagagac
     6121 aggctatcga tcccctcttc catcaaccat gcggcaaagt cttcatggtc ggacggaccc
     6181 tgaccgcaaa tcccgacata tttgccctgt ttcttcgcgg cacggatagc catcgacagc
     6241 aatgctttca ccgcatcgtt gcgctcatcg aacaattcag acaccacgcc ggagtcacga
     6301 tccagaccga gcgccagctg tgtcatatcg tttgagccaa ttgagaagcc gtcgaaatat
     6361 tcgaggaact gctctgccag caaggcgttg gacggaattt cacacatcat gatgattttc
     6421 agcccgttct cgccacgttt cagcccctga cgcgccagtt cttcaaccac cgctttcgcc
     6481 tgatctacgg tacgcacgaa cgggatcatg atctcaacgt tggtcagccc catgtcgttg
     6541 cgcactcgtt tcactgcttc acactccagc gcgaagcagt cgcggaagct gtcggaaaca
     6601 tagcggcccg cgccacggaa gccgagcatc gggttctctt catctggctc gtaacgctca
     6661 ccaccgacca ggttggcata ttcgttcgat ttaaaatcag agagacggac aatgacgcgc
     6721 ttcggataaa acgcggcacc cagcgtcgcg atcccttcag tcagacgacc aacgtaaaat
     6781 tcacgcggag aatcaaaacc tttcatcatc tcgcggattt cgttttgcaa ctgcggttcc
     6841 tgatcgtcaa actcaagcag tgcgcgtggg tggacgccaa tcatacggtt gatgataaat
     6901 tccagacgcg caaggcccac gccttcgttc ggcaggcagg cgaagtcgaa agcacggtcc
     6961 gggttaccga cgttcatcat cactttcaac ggcagatccg gcatcgtttc tacgctggag
     7021 cttttcacgc taaattccag caactccgca tagacgtaac cggtatcacc ttcggcacaa
     7081 gaaacagtga cgttctcacc gtctttcatc cgttctgttg catcaccaca gcccactacc
     7141 gccggaatgc ccagttcacg agcgatgatc gccgcgtgac aggtacgacc gccacggttg
     7201 gtgacgatgg cagatgcttt cttcatgatc ggttcccagt ccgggtcggt catgtcagtg
     7261 accagcacgt cgccaggttc gatgcggttc atttcgctga tgtcatggat gactttcacc
     7321 ggacccgcgc cgatgcgatg accgatagca cggccttcgg cgataatctt accctgtgaa
     7381 tgcagcgtat aacgctccat gacctgaccg cgtgagcgca cggtttccgg acgcgcctgc
     7441 acaatgaaca gtttgccggt gtggccatct ttcgcccact caatatccat cgggcgaccg
     7501 tagtgtttct caatttgtac ggcctgtttt gccagttcct gcacttcttc gttggtcagc
     7561 gagaagatgt cacgctgttc ctgcggtacg tcttcgattt taacctgctt gccgtgctcc
     7621 tgggtcggcg cgtaaaccat gcggattttt ttcgacccca tggtgcgacg cacgatagcc
     7681 gggcgattcg ctgcaagtgt cggtttatgg acgtaaaact catccgggtt aaccgcgccc
     7741 tgcacgacca tttcaccaag gccccatgcg gaagtgataa acaccacctg gtcaaagccg
     7801 gattcggtat caatggagaa catcacgcca gaagatgcga ggtcagagcg caccatccgt
     7861 tgaacaccgg cggagagcgc cacgccacgg tgatcgtaac cctggtgcac acgataagag
     7921 atggcgcgat cgttaaacag agaagcaaat acatgtttca ctgccacgag aacggcgtca
     7981 aaaccctgaa cgttgaggaa ggtttcctgc tgaccggcaa aggaagcgtc cggcatatct
     8041 tctgcagtag cggaggagcg caccgcaaaa gaggcgtttt cgtcatcggc ggaaagctgt
     8101 gcataagctt cgcggatggc attttccagc tcaggctgga agggagtgtc gataatccac
     8161 tggcggattt gcgcgcccgc tttcgcaagc tgggtaacat cgtcaatatc cgttttatcc
     8221 agcagttcat aaatgcgctg gtttacgccg ctttggtcca gaaactggtt aaacgcgtcg
     8281 gcggttgtgg cgaaaccatt cggaacggaa acacccattc cggaaaggtt agtaatcatt
     8341 tcacccaggg aggcattttt gcccccaacc ctgtctacat cattcatgcc gagttggtta
     8401 taccaaagca ccagcggtga cgagccattg ttggacatcg aacaatcctt ttgtgataaa
     8461 tgaacggttt gagaaacaca tttctgcgca tttatctttg catatttaac cggatgaaaa
     8521 aaacggtgaa tcgttcaagc aaatatattt ttttactttt taagactgat cccagcgttg
     8581 cgcaaacctg ctctattcga caatttccac ataaaccatc ggtataaacg aaaccataaa
     8641 aatgaaatgc tgttttcata aaaaataaaa ttgaaggttc attttataaa ccagacatta
     8701 cgtcctacgc ttctgtgcgt ttttaattta tgctttcata gaattatgtc tgcatcacgg
     8761 gaagaacaaa atggataatg ctgttgatcg ccacgttttt tatatttctg atggtacggc
     8821 aataactgcg gaggtattag gacacgcagt aatgtcacaa tttcccgtca ctatcagcag
     8881 cattacgctg ccgtttgtcg aaaatgagag ccgtgcacgg gcagtgaagg atcagattga
     8941 cgcaatttat caccagacag gtgtgcgccc gctggtcttc tactccatcg tgttgccgga
     9001 gattcgcgcc atcatcttgc aaagtgaagg cttttgccag gatattgttc aggcgctggt
     9061 tgccccgcta caacaagaga tgaaactgga tccaacgccg attgctcatc gtacccatgg
     9121 ccttaaccct aataatctca ataaatatga tgcgcgcatt gcggcgattg attacaccct
     9181 cgcccacgat gacggcattt cgttgcgcaa tctggaccag gctcaggtga tcctgctcgg
     9241 tgtttctcgc tgtggtaaaa cccccaccag tctgtatctg gcaatgcagt ttggtatccg
     9301 cgcggcaaac taccccttta ttgccgacga tatggacaat ctggtgctac ccgcgtcgct
     9361 caaaccgctt cagcataaat tgttcggcct gactatcgac ccggaacgtc tggcggcgat
     9421 tcgcgaggaa cgtcgggaga acagccgcta tgcctcgctt cgtcagtgca ggatggaagt
     9481 cgcggaagta gaagcgttgt accgtaaaaa tcagatcccg tggattaaca gtaccaatta
     9541 ttcggtagaa gagattgcca ccaagctcct cgatatcatg gggcttagcc gccgaatgta
     9601 ctagagaact agtgcattag cttatttttt tgttatcatg ctaaccaccc ggcgaggtgt
     9661 gacacacctc gcacttgaaa tcagctgcga ttggtttatc gtgatgcgca tcacttcccg
     9721 gtagtcctgc cgtagaagca acaaatttct gagacttgta atgaacagaa ctgacgaact
     9781 ccgtactgcg cgtattgaga gcctggtaac gcccgccgaa ctcgcgctac ggtatcccgt
     9841 aacgcctggc gtcgccaccc atgtcaccga ctcccgccgc agaattgaaa aaatactgaa
     9901 tggtgaagat aagcgactgt tggtcattat tggcccctgc tcgatccacg atctcaccgc
     9961 tgcaatggag tacgccaccc gtctgcagtc gctgcgcaac cagtaccagt cacggctgga
    10021 aatcgtaatg cgcacctatt ttgaaaaacc acgaactgtt gtcggctgga aagggctaat
    10081 ctccgatccg gatttaaacg gcagctatcg ggtaaatcac ggtctggaga tggcgcgcaa
    10141 attactttta caggtaaatg agctgggtgt ccctaccgcg actgagttcc tcgatatggt
    10201 gaccggtcag tttattgctg atttaatcag ttggggcgcg attggcgcac gtactaccga
    10261 aagtcagatc caccgtgaaa tggcttcggc actctcctgt ccggtaggtt ttaaaaatgg
    10321 taccgatggc aactcgcgga ttgccgtgga tgctatccgc gcagcccgcg ccagccatat
    10381 gtttctctcg ccagacaaaa atggtcagat gaccatctat cagaccagcg gcaacccgta
    10441 tggccacatt attatgcgtg gcggcaaaaa accgaattat catgccgatg atatcgccgc
    10501 agcctgcgat acgctgcacg agtttgattt acctgaacat ctggtggtgg atttcagcca
    10561 cggtaactgc cagaagcagc accgtcgcca gttagaagtt tgtgaggata tttgtcagca
    10621 aatccgcaat ggctctacgg cgattgctgg aattatggcg gaaagtttcc tgcgcgaagg
    10681 aacgcaaaaa atcgtcggcg gtcagccgct cacttacggt caatccatta ccgacccgtg
    10741 tctgggctgg gaagataccg aacgcctggt cgaaaaactt gcctctgcgg tagatacccg
    10801 cttctgaatg cgtgcccatt cctgatggga tgggcatttc tgcgcaactt gttgtcttct
    10861 cagcacatta ctgcttgctc tggtcagcca taatattgat aataagaatc attgttatat
    10921 caattattat taatttttat gcgttatacg gatagcagaa aactcacgcc tgaaacggat
    10981 gccaatcaca agaccgcttc cccgcagcct attcggcgaa tttccagcca gacactgtta
    11041 ggtccggatg gcaaactgat tatcgatcat gacgggcaag aatatctgct ccgtaaaact
    11101 caggctggca agctgctgtt gaccaagtag cctttaactc gagcagctga cttccagccg
    11161 tttaccccag tcaggtggac gactgacata gtcatcagct ctgtcgctga aaggatttcg
    11221 caacgcctca tgcaggcggt gcaattccgt catatcaccc ttttctgccg cttcaatcgc
    11281 ccgttgcgcc aaccagttgc gcaacaccag agctgggtta acgctttgca tcagctgctg
    11341 acgctcacta tcgctaacct cgtcttgttg caaacgcccc cgataacggg caaaccagtc
    11401 atcaaatgcc gcacgatcaa taaactcatc acgtagcggt gacgccgcgc tgtgctgctc
    11461 ggtcagactc agcatgcgga atgtgcgggt ataatcgctg cgctctcgcg ccatcagact
    11521 gaataattca ttcagtagcg cgttatcctc tttttgctcc gtcatgaagc ccagtttctg
    11581 ccgcatccgt tgtccataat gcgtcaacaa aacctgctga tagctgtcca gggcctcatt
    11641 cagggcatct acggcaacaa atggtgacaa tgtctgcgcc agacgctgta aattccacaa
    11701 cgcgacagca ggttgattat caaagctata acgcccttga tgatccgagt gattacaaat
    11761 aaaaccgggt tcgtaatcat ccagaaaacc aaacggcccg taatcaagcg tcagccccag
    11821 cagcgacatg ttatcggtat tcatcacccc atgagcaaag ccgaccgtct gccattgggc
    11881 aattaacgat gcggtacgtg cgacaacatc gctaaaccag aggcggtatt tgtcctcatc
    11941 atcttcaaga tatgaccagt aatgacgaat ggcaaagtca gctaactgac gaaccttttc
    12001 cggctcgcgg cgatagtaaa aatgttcgaa atgaccaaag cgcagatgac ttggtgccac
    12061 acgcatcagc atcgcgcctg gctccaccgt ttcccgatac actggcgaat cgctggtgac
    12121 gatacttaac gcgcgggtcg tcggaatgcc cagataatgc atcgcctcac tggcgagact
    12181 ttctcgtatc gtcgaacgta aaaccgcccg tccatcaccc attcgcgaat aaggcgtcag
    12241 gccagcacct ttcagatgcc agtccattgt agtgccatca gcaagcagtt gttcgccgag
    12301 taaaataccg cgcccatcac ccagttggcc cgcccagacg ccgaactgat gaccactgta
    12361 aacctgcgcc agtggtgaca taccaggcag tagggtttcg ccgccccaga cacctgcgcc
    12421 atttttaaac agcgacgatg gaatactcag cgtgttagcg agttcggtat tatgccaaat
    12481 cagccgggca ttatttaaag gcgtagggga aagtgctgta taggttgctg gcaattcatc
    12541 gcgccagcgg gtaataaaag acagggtcat agatcctcct gtttaatagt gtagacggtt
    12601 actctcgtct taaacaccag caaacaggag ggttatcgct gaaccaacgt cgttatctga
    12661 tttataggaa cggcaggcca caggcatccc tggagagcat ggaaatcgaa gggggtaatc
    12721 tgtgccaata tttctgtcgt atcaatgccc cccgcgatga tgcaattgca acaaggggaa
    12781 atttgcgcct ggatcgcacg aataaacggt tcgaaggagc gatgcgtgat ttgttgctga
    12841 ataaaacttt tatccagcat gacgcgggtg aaaagaccat caaaaaccgc tttcattgtg
    12901 ctattacctg ctcccaaatt gcctaacaca aggggataca tttgcgataa ggataaaaga
    12961 tcgcgattat cttttccttc attgagatgc gggtaattct cattaatcaa cagttcaata
    13021 aacggatatt ttaatagttc gcctgcgtaa ttatccctct ctaataataa tgtagccact
    13081 tgtggcgtta aattaagcca ggcaaagagt ttatgttgaa taaaaaaatg ttggcatgat
    13141 tttagtaatt ccaactgctc tgaaaataat tgccaatgtt gctcctcggt aagttgcgct
    13201 ataacccgac tcgtcgggat acgaaccgta ccatcttcac tggagaaatg ggtaattaac
    13261 tcaacgccga caaggtcttg ctggttatct cttatcggca ggaaatagca atcagaatga
    13321 taaagattct ccaaaaaaat cttcatggta accgtccctc tcgaaggatg tttttcagta
    13381 tccggctaaa agggatgagg ccataagaca taacaattac ataaggagta actttcattt
    13441 gttccatgtt aaccactttt tcaggggtcc tttttttaga ttatcctgat tataaacgaa
    13501 taatccattt acggaatttt tgtctgcaaa atactactgt attcaggagt aaatggacgg
    13561 cgatgtatca tcgccgtcca tttttcgaca tactaaattc gtctcgcctg ccagaaattt
    13621 ttctgccaat agacattatc aagtgaggaa cgcatcactc ccttgctggt agaggcgtgg
    13681 ataaattggt tgttggtatc gtaaatacct acatgcaaac cattttgtcc ggaacccgtt
    13741 ttgaaaaaga ccaggtcacc aggcagcaac tcgtctttat caatttgcgt gccgatagag
    13801 gcttgttctt tggtttctcg gggcagctgc aaatcgaaac gatcgcgcat cgttacaacc
    13861 acaaatcccg aacagtccac accgcgccgc gtcatgccac cataacgata cggcgtgcca
    13921 tgccagcttt gtagctggtc gttcaaaccg gcaataacgg taatcgaatc agaaagtctg
    13981 gcatttggcg gcggtgcttt atggtggcta cacccggcca gaaacagtgc tgtgatcaaa
    14041 ataaggcaga aacgcattcc gtacggttcc tctgtttttt attcttgcat taatttagcg
    14101 tcgtaattac ccgattttca agatactaat gaaatcagat ggtcgaaatc agcattctgt
    14161 gaccttcgat atccagacgg cgaaaattca tcccataggc ctgcgccaga tttggcggcg
    14221 tgagcacctc ttctctgcgt ccactggcca gcatttttcc accttttagc aaccacgccc
    14281 gatgcgcatg acgcaatgtg tggttgagat cgtgactgct catcacaatc gccagtcctt
    14341 gctgacacag agcgctcaga attttgtcta acgcactttg ttgcgcaaca tcaagactgt
    14401 tcatcggctc atcaagaagc agcaattgac ctgcgggatt ggcttgtggt gtgatttgca
    14461 gcaccaccgc agcaagacgt acgcgttgcc attcaccgcc ggaaagttga ttggtgctac
    14521 gtccgagttt gtcatcaaga gccagcgccc ctgcgacatc attcagtagt tcggtacgcg
    14581 ttttatcgtg ctgatgcagt gtcaggtagt gccagaccgg cattgcaaac ggcggcgtct
    14641 gctgttgtga aaggtaggcg cgatgcagcg cgagttttgt tgcggaccat gcttccagtg
    14701 gttgccccgc gaactgaatg cttcccttac cgctggtcat tccggccatt cgtgcaagca
    14761 aggtactctt acccgcgcca ttcggcccca ccaggtgcag gatctcccca gcccgaacct
    14821 cgccagaaag cggccccagg cgggtagttt ccgcaacatc ctgtaactgc atcacaatag
    14881 acattatttt gccaacgcca gtttaatgct ttccatcaca atgggatctt caggcgtcat
    14941 atccggggaa aaacgctgga tgaccaaccc gtccctgcca actaaaaatt tctcaaaatt
    15001 ccataaaata tcatcagggt acagcggtgc acgacctttg ctgaccatac gggcatagaa
    15061 tccgctctct tccggcgcga ctgcggtcgg cgctgcggca atcaattttt gatacagcgg
    15121 atggcgtcct tcgccattaa cttcaatctt actgaacatc gggaacgtca ccccccatgt
    15181 ggtggtacag taagttttaa tctcttcatc gctgcccggt tcttgttcca gaaactggtt
    15241 gcacgggaat cccagcacca caaaacctcg atcggcccag gctttctgaa tattctccaa
    15301 ctgctcatat tgcggcgtta agccacactt tgaggcgaca ttgacaatca acagcacatt
    15361 accagcgtac ttctccagcg tggtcacttc accgtcgata tctttcacta cggtcgtcag
    15421 aatggaatct tgcatcgttt ctcctgggtg tggtcagtaa aaatcttagc ttttaatcat
    15481 agaccgtctt tttgcggcta acgtcttgct tttaacaata accagataaa caccggcgca
    15541 cccaacgttg cggtgaccac gccaataggc agctctgcgg cagctaatgc caggcgcgct
    15601 acaatatcgg ccagcagcaa tgcgctcgcc cctgccagcg cgcagccggg aagtaatacg
    15661 cgatgatcgg ttaaaccaca caaccgaaga atatgtggga tcaccagacc aataaagccg
    15721 atagcacccg ccagcgccac actgacgcca accatccagc cggtcgcggc caccagcaca
    15781 ttgcgccaga accacagggg taaacccagt tgccgcgccg agatctcgcc aagtgctaac
    15841 atattcatcg gcctggactg acaacagatc cacaacaaca cggggatcaa tgccagcatc
    15901 agccagcttt gccgccagtc tacgccgcca aaaccgccca tcatccagta catcagctga
    15961 cgtaaatcaa cagaggtgga aaagtagata gcccacgtca ttagtgcgct acagataatc
    16021 cctaatgcaa cgccagccag caataaccga ctggtcgaaa gatgacgacg ggcgaaacgt
    16081 aagagtatta aagtgatgat aagcgcgcca gcaatcgcac acagccctag cgcccagttg
    16141 gggagttgcc cttgcccaag caataccgcg gcgataagcc ccacgcctgc gccgttagag
    16201 acgccaagta gtccaggttc tgccagagga ttttcaaaca acgcctgcat tacagcgccg
    16261 gatatagcca gcgccgcacc aaccagcaat acagccagcg tacgtggcag gcgaatttgc
    16321 cagacgaaca gttcgccacg aggagtaaac cagtcacctg gcgagatcca ttgttcaccg
    16381 gcgcaaaggc ttaagagaag cgccagcagc atcaaaactg acaggcataa taaccagcga
    16441 atattttgtc gctgttgttg gcgggcaagt gtcagcatgg tatccgttct gctgaagtgt
    16501 catggcgttg attttacggt gactcttcga cagtgaaaag aaaaaaggcc gcagagcggc
    16561 ctttttagtt agatcagatt actcgtcttt gggcgaagcg ttttcgaccc ggctttttaa
    16621 cttctgcccg ggtctgaagg tcaccacgcg ccgtgctgta atgggaatat cctcgcccgt
    16681 tttcgggtta cgtcccgggc gttgattctt atcacgcaga tcgaagttac caaaaccaga
    16741 gagtttcacc tgttcgccgt tttccagagc gcgacggatc tcttcgaaaa acagttcaac
    16801 cagttctttg gcatcccgct tgctaagccc aagcttatca aacagatatt ctgacatttc
    16861 agcttttgta agcgccatag gttcaatccc tcaatgatgc ctggaatcgc tcttttaatg
    16921 cctctacaca tttggcgacg gtagcggcaa tctcctcttc ttcgagtgta cggctggtat
    16981 cttgcaggat caggcttatg gcgaggctct tatacccctc cgcaacaccc ttaccgcggt
    17041 acacgtcaaa taagtttacg ccaactacct gatttacgcc aactttctta cattcggata
    17101 aaatatccgc tgcgggaacg ttttctgcga ccaccaccgc gatgtcacga cggttcgccg
    17161 ggaagcgaga aatctcgcgc gcctgaggca ccacgcggtc tgcgagcttg ttccactcca
    17221 gttcgaacac cagagtgcga ccgttaagat ccagtttacg ttccagttca ggatgaacaa
    17281 ccccaacaaa accaatacgt tcacctttca gataaatcgc tgcggattgc cccggatgca
    17341 gtgccggatt cgcttctgca cggaactcaa cctcattcag tttaccggtc aggtcgagaa
    17401 cggattcaag atcgcctttc aaatcataga aatcaacggt ctcttttgcc aggttccagt
    17461 gctcttcgta acggttaccg caaatcacac cggctaacat cagatcctga cgaatgccca
    17521 acggtgcctg agtatctggt acgaaacgca gaccgctttc gaaaatgcgc acacggttct
    17581 gctgacggtt ctggttgtac accacggttg ccagcaggcc ggtccacaga gaaagacgca
    17641 ttgctgacat ttcaacagag atcgggcttg gcagcagtaa ggcttcaacg cctggatgga
    17701 tcatctgctg cactttcgga tcaacgaagc tgtaggtgat cacttcctga tagcctttgt
    17761 cgttgagcag cgttttcacg cgcttgagcg acaggtcagc ttcacggtga gtacccataa
    17821 tcaggcttgc ctgtaccggc tcatccggga tgttgttgta gccgtaaaca cgcgcgactt
    17881 cttcgaccag atcttcttca atctccatat cgaaacgcca gctcggcgca actgcctgcc
    17941 actcgtcttt gccttcggtc acttcgcagc cgagacgacg cagaatgtca gttacctgct
    18001 catccgcaat atgatggccg atcaggcgat ccagtttgct acgacgtaaa gtgatggttg
    18061 cacgcttcgg cagcgttgct tcgttggtga tatcaattac cggaccagcc tcaccaccgc
    18121 agatgtcgat cagcagacgg gtcgcacgtt ccatcgcttt gtgctgcagt gccggatcaa
    18181 cgccacgctc ataacggtga gacgcatcag tatgcaggcc atgacgacga gcacgaccgg
    18241 tgatagacag cgggctgaag aaagcgcatt ccagcaagac gttttgcgtt tcgtcattca
    18301 cgccagagtg ttcgccacca aagatgccgc ccatcgccag cgccttgttg tggtcggcga
    18361 tgaccagagt gtcagcattc agcttcgctt cagtgccatc aagcagtacc aacgtttcgc
    18421 cctctttcgc catccgcacc acaatgccgc cttcaatgcg atctttatcg aaagcgtgca
    18481 tcggctggcc cagttcgagc agtacatagt tggtgacgtc aacaactgca tcgatagaac
    18541 ggatcccgca acgacgcagt ttttctttca tccacagcgg agttggcgct ttaacgttaa
    18601 tgccttttac cacacggcca agataacgcg ggcaggcttc cggcgcttcg actgtaatcg
    18661 gcagcgtgtc gtcgatggtc gcaccaaccg gaacgatttc cggttcaacc agcggcagct
    18721 gattcagcac ggcaacgtca cgcgcaacac caatgatacc taaacagtcg gcacggtttg
    18781 gcgtcacgct gatttcgatg gtgttgtcat caagtttcag gtattcacgg atgtcggtgc
    18841 caatcggcgc atccgcaggc agctcgataa tgccgctatg atcgtcagaa attcccagct
    18901 cagaaaagga gcacagcatc ccttcagacg gttcaccacg cagtttcgcc gctttaattt
    18961 tgaaatcacc cggcagaaca gcaccaatgg tagctaccgc cacacgcagg ccctgacggc
    19021 agtttggcgc accgcagacg atgtccagca ggcgatcgcc gccaacattc acttttgtca
    19081 cacgcagttt gtcagcgttc ggatgctgcg cacactcaac cacttcacca acgaccacgc
    19141 cgtggaagct accggcaacc ggttctacac cgtcaacttc caggcccgcc atagtgattt
    19201 gattcgccag cgcatcgcta tcaatcgccg ggttcaccca ttcgcgtaac cacagttcac
    19261 tgaatttcat aatctattcc tgccttattt aaactgtttg aggaaacgca gatcattttc
    19321 gaagaatgaa cgcaggtcgg tgacgccgta acgcaacata gtcagacgct ccatccccat
    19381 cccgaaggcg aaaccagagt aaacttccgg gtcgatgcca acgttacgca gcacgttcgg
    19441 atgcaccatc ccgcaaccca gcacttccag ccatttaccg tttttaccca tgacgtcaac
    19501 ttctgcagaa ggctcggtga acgggaagta ggaaggacgg aagcgaatct gcaaatcttc
    19561 ctcaaagaag ttacgcagga aatcgtgcag agtgcctttc aggttggtaa agctgatgtt
    19621 ggtatcaaca atcagacctt ccatctgatg gaacatcggc gtgtgagtct ggtcgtagtc
    19681 gttacgataa acgcggccag gcgcaatgat acgaatcggt ggctgctggg ctttcatggt
    19741 gcggatctgt acgccagagg tctgggtacg cagcaggcgg gtagtgtcaa accagaaagt
    19801 gtcgtggtca gcgcgcgccg ggtggtgacc aggaatgttc agagcatcga agttatgata
    19861 atcgtcttcg atttccggcc cggttgccac ggtaaagcca agctcaccga agaaactttc
    19921 gatacggtcg atagtacggg taaccggatg cagaccgccg ttttcaatgc gacgacctgg
    19981 cagagagaca tcaatcgttt ccgccgccag acgcgcattc agtgcagcgc tttccagttc
    20041 cgctttacgc gcattcagcg cctgctgaac ctgctctttc gcttcgttga taaccgcacc
    20101 agctgccgga cgctcttctg gcggcagctc acgcagggtc gtcatctgaa gggttaagtg
    20161 ccctttttta cccaaatatt cgacgcgcac attatctaac gcggcaacat ctgacgcctg
    20221 gctaatggcc gccttcgcac tggcaaccag ttctgcgaga tgtgacatgg ttttcctcat
    20281 tgtgtcagtg gtgacactgg ttcgttggac ttagagccta tcccatcagg ctattttact
    20341 tgccattttg gtcccgggca gtgctcgaaa ttctcacgta cttaaatacg ctccggtttc
    20401 tccgcgctgg ccgtgtccag actggctgcg acaattacac ctgatgagac aggcttttta
    20461 tttttcaaaa cgcgcataca aaaaaagcct ccactgggag gctttcaggc gctgttttcc
    20521 gtttctcttc tcacgcgcta gcctcctgga ttcaggtgct aaagtaaaaa aagaagcgga
    20581 aaatagcagc attcattgct tgcgttacct tttggtactc ttcaaaagac ctttattgaa
    20641 aaggctacgg cgataaaagt caatgttttg atggcgttga aacgaaaaga gggagactag
    20701 ctccctcttt caactggctt atgccagagc tgctttcgct ttttcaacca gagcggtgaa
    20761 cgctactttg tcgaatactg cgatatcagc caggatctta cggtcgattt caacagaggc
    20821 ttttttcagg ccattgatga atttgctgta agaaataccg ttctgacgtg ctgctgcgtt
    20881 gatacgcgca atccacagtt gacggaactg acgcttacgt tgacgacggt cacggtaagc
    20941 atactgacca gctttgataa cagcctggaa ggcaacgcgg tatacgcgag aacgcgcacc
    21001 gtagtagcct ttagcttgtt tcaaaatttt cttgtgacgt gcgcgtgcaa taacaccacg
    21061 ttttacgcga gccatatgtg ctctcctgta tctatattct aattaaaaag ttaaaaacgt
    21121 taacggctta tgcgtacggc aggcacgcga ttaccaggcc cagatcgcct ttggaaacca
    21181 tggctttcgg acgcaggtga cgtttacgtt tggtcgcttt tttggtcaga atgtgacgca
    21241 ggttagcgtg cttgtgctta aaaccacctt taccggtttt tttgaagcgc ttagcagcac
    21301 cgcgtacggt cttaattttt ggcattttaa taacttccac ttcgcattgt taataaacga
    21361 aacaaaggcg aacaaagcct gtgaagcccg aaggctccac agacagtgct acttgaaggc
    21421 cttactgttt cttcttagga gcgagcacca tgatcatctg acggccttcg atcttcgttg
    21481 ggaaggattc gaccactgcc agttcttgca aatcgtcttt cacgcgatta agcacttcca
    21541 taccgatttg ctggtgcgcc atctcacgac cgcggaaacg cagcgtgatt ttggctttat
    21601 caccctcttc gagaaagcga atcaggctgc ggagttttac ctgatagtcg ccttcatctg
    21661 taccaggacg gaatttaatt tccttaacct ggataacttt ttgctttttc ttctgttcct
    21721 tagaagactt gctcttttca tagaggaatt tgccgtaatc cattatacga caaaccggcg
    21781 gctcggcgtt agggctgatc tcgactaagt ctactccggc ttcttctgct ttctccagag
    21841 cttctctcag actcacaata ccaagctgct cgccttccag acctgttaag cgaacttcct
    21901 gggcgcgaat ttcgccattg atacggttag ggcgcgccgt ttgaactcgt tttccgcctt
    21961 taatacctta ttcctccaat tgtttaagac tgcggctgcg aatctcttgt tgcagcttct
    22021 cgatcacttc atttacgtcc atgcttccca ggtctttacc acggcgggtg cgaacggcaa
    22081 ctttgcctga ttccacctct ttatcaccac agaccagcat atatgggacg cgacgcaaag
    22141 tgtgctcgcg gattttaaag ccaatcttct catttctcaa gtctgcttta acacgaatgc
    22201 ccgcatttga tagtttttgc gtcaattcgt taacgtattc agactgtgaa tcggtaatat
    22261 tcatgataac aacctgaact ggcgcaagcc aggtcgggaa gaaaccagcg aattcttcgg
    22321 tcaggatacc gatgaaacgt tccatcgatc ccagaattgc gcggtgaatc attaccggta
    22381 ctttacgttc attgtcttcg cctacataag aagcgctcag acgagaaggc aaagagaagt
    22441 ccagctgtac tgtaccgcac tgccatgcac gatcgaggca gtcatacagg gtaaattcaa
    22501 ttttcggacc gtagaaagcg ccttcaccca gttgatattc aaacgggatg ttgttttctt
    22561 ccagcgcaac cgccaggtcc gcctcagcac gatcccacat ttcatcactg ccaatacgtt
    22621 tttcaggacg agtggagagt ttgacgacga tcttctcgaa gccaaaagtg ctgtacatat
    22681 catagactaa acggatacat ccgttaactt catcgcgaat ttgttcttca gtacagaaga
    22741 tatgcgcgtc atcctgggta aatccacgca cgcgcatcag gccatgcagc gaacctgacg
    22801 gctcattacg gtggcagcta ccaaactcgg ccatacgcag cggcagatcg cgataagact
    22861 tcagcccctg gttgaaaatt tgtacgtgac ccgggcagtt catcggctta atgcagtatt
    22921 cacggttctc agaagacgtg gtgaacattg catctttgta gttgtcccag tgaccggttt
    22981 tttcccacag gacacggtcc atcatgaacg gacctttaac ttcctgatac tggtactctt
    23041 tcagtttaga acgaacaaac acttctagtt cacggaagat ggtccagccg tcgttgtgcc
    23101 agaataccat acccggcgct tcttcctgca tatggtacag gtcgagctgt ttaccgattt
    23161 tacggtggtc gcgtttcgcg gcttcttcca ggcgctgcag gtaagcgtta agcgcttttt
    23221 tgtctgccca cgccgtaccg taaatacgtt gcaacatttt gttgttgctg tcgccacgcc
    23281 agtaagcccc tgccgttttc attagtttga aatgatggca gaaacgcatg ttcggtacgt
    23341 gcggaccgcg gcacatatcg acatattctt catggaagta cagacctggc ttgtcatcat
    23401 gggcgatgtt ttcgtcaaga atggagactt tgtagctctc cccacggttg gcgaaagttt
    23461 cacgcgcttc gtgccagctg actttcttct taatgacgtc gtagtttttc tcagcaagct
    23521 catgcatccg cttctcgagt gcttcgacat cttcctgggt taacgtgcgg tcaagatcaa
    23581 cgtcgtaata aaaaccgttg tcaataaccg ggccgattgc cattttggta tgcggccaaa
    23641 gttgtttaat cgcgtgccct aacaggtgcg cacaggagtg acgaatgatc tccagaccgt
    23701 cttcgtcttt ggcggtaatg atggacagtt gtgcgtcgtt ttcaatcaga tcgcaagcat
    23761 caaccagttc gccattaacg cgccctgcga tacaggcttt cgccagacct ggaccaatgt
    23821 ccagcgcaac atccatgggg cttacagcgt ggtcgtaatg gcgttggctg ccatcaggaa
    23881 gagttataac aggcatttta tatccttatt tgcagtggtg acccacacga aagatcacat
    23941 acaaagaaaa atttgtttat taacagttca ttgcgaaacc atctagccaa caaatgctaa
    24001 attggttcgc aatcaggtac acaaatgaaa gacattaacc tctcaccttc cacccggttg
    24061 agcgatgtta acactaaaaa aagggagatt gtacctttcc gtttcacatt gattcgattc
    24121 gcaattcgtt tgtagtgagt acaccagcaa aacgcttaaa gaaccactga taacatcacg
    24181 gtgcgtctga aaggtttact gcgagcggat ttacagcagc ataaactgaa tccagcaaag
    24241 ttcgttgcgt gatttggcgg tagtatttta aacgctcatg agattaatga cgaagtggtc
    24301 atatcacaat gataaaagtg acacaattct tataacaatt tttcgtgcac atttcgttct
    24361 ggcgataata attaatcatc atcctcaaaa cctccagata tatataaggc gaatagatta
    24421 tgagtcaaaa cgatatcatt atcagaactc attataagtc tcctcataga ttgcacatcg
    24481 atagcgacat accaacacct tcatcagagc ctattaataa atttgcgcgc cagctcatca
    24541 ccctacttga tacctctgac ttaagttcga tgctgtcata ctgtgttact caggaattta
    24601 ccgcaaactg tcgaaaaata tcacaaaatt gttattccac tgcccttttt accattaacc
    24661 ttgccacttc acccatccat gcagaaaata tactcattac attacactat aaaaaagata
    24721 tcatttcctt attactggaa accacgccta ttaaagctaa ccatttgcga agcatactgg
    24781 attatattga acaggaacag ttaactgctg aagatcgtaa ccattgtatg aaactgtcta
    24841 aaaaaatcca tagagaaaaa actatacacc caacagtaaa tctcaatggt agtgcatgtt
    24901 ttttgcaatc tccttctgac gctatttttt gtcgccatct gtcattgcaa tacgcccttg
    24961 attcattgag aaatggaaaa ggcaaagtca acctgattaa acattactcc tccgttgaat
    25021 ccatacagca gcatgtcccc ttagtccggg acgcggagtt cagagcatta cttcgccatc
    25081 ctcctgcagg gagtcgcgtt atcgcgagta aggattttgg cttcgcttta gattttttct
    25141 tttgtcgaat gatggcaaac aatatcagtc atatgtccgc gattttatat atagacaatc
    25201 atactttgtc agtaaggcta cgaataaagc agtcagtgta tggacaatta aactatgttg
    25261 tgtccgttta cgacccgaac gataccaacg ttgccgtcag agacacccac aggacagcac
    25321 ggggctttct ttcgcttgat aagttcatca gttcaggtcc cgatgctcag acctgggctg
    25381 ataggtatgt tcgcaactgt gcaattgcta ttctgcccct attacctgtg ggagttccag
    25441 gggctatttt cgcgggtatt gcatcacgaa tgccatttgc ccctatacat ccatcggcaa
    25501 tgttgttaat aatggctaca ggccagactc aacagcttat tacattattc aaacagttgc
    25561 ccatactccc tgaaaaagaa atcattgaaa taataactgc gcagaatagc gttggtacac
    25621 ctgctttatt tttggctatg atgaacggac atactgacaa cgtgaaaata tttatgcaag
    25681 aaattcagtc actggtagat aatcacatca ttcatgaaga taacctggtt aaattactgc
    25741 aaactaaaag tgctaacgaa acacctggac tctatatctc catgttgtat ggattcgatg
    25801 aaataatcga tatctttctg aatgcattaa ccactccaat agcacaagaa cttttaaaca
    25861 aaaaaatggt gatggatatt ttagccatga aaacacgtga tggtgaacca gggttatttg
    25921 ctgcaatgga aaataatcac cctttgtgtg tcacacggtt cctctctaaa gtttatggga
    25981 tcgccgttaa atataaactc agcaaaatca acatcatgga cttattaaaa ggcgcgacag
    26041 cacagggaac ccctgcatta tacatcgcca tgagcaaggg taatgaagac gtcgtgttat
    26101 cttatatatc gacgctgggt gcttttgcaa aaaaaacatt cttttagtca acatcagtta
    26161 tttacactat tggccgctaa aaatcatgac aacatgtcag ctgttcatat agccattcat
    26221 cataagcatt ataaaactgt agaaacatat tatgctgcta taaatgtcat cagcca
//
LOCUS       DAZFJF010000068        26208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFJF010000068 DAZFJF010000000
VERSION     DAZFJF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.068"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(250..780)
                     /locus_tag="ACHKAU_002945"
     CDS             complement(250..780)
                     /locus_tag="ACHKAU_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531968.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            971..1219
                     /locus_tag="ACHKAU_002946"
     CDS             971..1219
                     /locus_tag="ACHKAU_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5531969.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            1221..3311
                     /locus_tag="ACHKAU_002947"
     CDS             1221..3311
                     /locus_tag="ACHKAU_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO5531970.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            3382..4314
                     /locus_tag="ACHKAU_002948"
     CDS             3382..4314
                     /locus_tag="ACHKAU_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5531971.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            4317..4538
                     /locus_tag="ACHKAU_002949"
     CDS             4317..4538
                     /locus_tag="ACHKAU_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531972.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            4551..4805
                     /locus_tag="ACHKAU_002950"
     CDS             4551..4805
                     /locus_tag="ACHKAU_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531973.1"
                     /translation="MNIQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            4807..5088
                     /locus_tag="ACHKAU_002951"
     CDS             4807..5088
                     /locus_tag="ACHKAU_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO5531974.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            5085..5357
                     /locus_tag="ACHKAU_002952"
     CDS             5085..5357
                     /locus_tag="ACHKAU_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531975.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFTELVAMKASALMKDMDMSSGVIKGNEFNQQG"
     gene            5362..5655
                     /locus_tag="ACHKAU_002953"
     CDS             5362..5655
                     /locus_tag="ACHKAU_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531976.1"
                     /translation="MSKVRVIFEFDHVMHEVKPAGNDSEEITEGVTATVKIERDTENR
                     PAGPCDVYAQILKYHSPTIIQFLTDELQGSMQAMGVSSSVERRSVQNGPDTLQ"
     gene            5667..6197
                     /locus_tag="ACHKAU_002954"
     CDS             5667..6197
                     /locus_tag="ACHKAU_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5531977.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            6295..6837
                     /locus_tag="ACHKAU_002955"
     CDS             6295..6837
                     /locus_tag="ACHKAU_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531978.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            6841..7374
                     /locus_tag="ACHKAU_002956"
     CDS             6841..7374
                     /locus_tag="ACHKAU_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531979.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            7374..7889
                     /locus_tag="ACHKAU_002957"
     CDS             7374..7889
                     /locus_tag="ACHKAU_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531980.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            7893..8444
                     /locus_tag="ACHKAU_002958"
     CDS             7893..8444
                     /locus_tag="ACHKAU_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO5531981.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDAVLRELTP"
     gene            8441..8626
                     /locus_tag="ACHKAU_002959"
     CDS             8441..8626
                     /locus_tag="ACHKAU_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531982.1"
                     /translation="MIKRLVFFAIFVPVLGVGFIFAITGNLSMMPDIWFFIRMSLFLF
                     IMNLLIDIYIRITGKHK"
     gene            8665..8997
                     /locus_tag="ACHKAU_002960"
     CDS             8665..8997
                     /locus_tag="ACHKAU_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531983.1"
                     /translation="MSIELRSSYEYRKFLIAGGMKPEDVEKIVSFMDKECDKRDVSEI
                     IMDDMILDSVVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            8990..9187
                     /locus_tag="ACHKAU_002961"
     CDS             8990..9187
                     /locus_tag="ACHKAU_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531984.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            9177..9473
                     /locus_tag="ACHKAU_002962"
     CDS             9177..9473
                     /locus_tag="ACHKAU_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531985.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHVRWDEAEKAFHVRKKVNTV"
     gene            9470..9979
                     /locus_tag="ACHKAU_002963"
     CDS             9470..9979
                     /locus_tag="ACHKAU_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO5531986.1"
                     /translation="MRGKLISAIHVAKRELALDDEAYTFALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMEMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            10049..10474
                     /locus_tag="ACHKAU_002964"
     CDS             10049..10474
                     /locus_tag="ACHKAU_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO5531987.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDILKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            10546..11046
                     /locus_tag="ACHKAU_002965"
     CDS             10546..11046
                     /locus_tag="ACHKAU_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5531988.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            11081..11509
                     /locus_tag="ACHKAU_002966"
     CDS             11081..11509
                     /locus_tag="ACHKAU_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531989.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASVLVPDDVADRVRRAVCEIRACEAGADPR"
     gene            11493..11711
                     /locus_tag="ACHKAU_002967"
     CDS             11493..11711
                     /locus_tag="ACHKAU_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531990.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            11722..11949
                     /locus_tag="ACHKAU_002968"
     CDS             11722..11949
                     /locus_tag="ACHKAU_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5531991.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            11930..12238
                     /locus_tag="ACHKAU_002969"
     CDS             11930..12238
                     /locus_tag="ACHKAU_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO5531992.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            12235..12525
                     /locus_tag="ACHKAU_002970"
     CDS             12235..12525
                     /locus_tag="ACHKAU_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO5531993.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            12528..13109
                     /locus_tag="ACHKAU_002971"
     CDS             12528..13109
                     /locus_tag="ACHKAU_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO5531994.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            13109..14773
                     /locus_tag="ACHKAU_002972"
     CDS             13109..14773
                     /locus_tag="ACHKAU_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5531995.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            14773..16362
                     /locus_tag="ACHKAU_002973"
     CDS             14773..16362
                     /locus_tag="ACHKAU_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO5531996.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLSIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVRDQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPGKQEKTEQTALAARLPEAKS
                     TPRDELDDMGDAVPARLLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            16346..17671
                     /locus_tag="ACHKAU_002974"
     CDS             16346..17671
                     /locus_tag="ACHKAU_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO5531997.1"
                     /translation="MAQGIDLGYAATLASKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKAARLDVLTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGNPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFAQLPAMLAAPDAVLWDHVHQNLLYITETRDG
                     TAKIAVNAPYSVKRQPDKLDVVINAYRVSRNTLIDAVQGGKMEILEGKL"
     gene            complement(17671..17758)
                     /locus_tag="ACHKAU_002975"
     tRNA            complement(17671..17758)
                     /locus_tag="ACHKAU_002975"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            17797..18270
                     /locus_tag="ACHKAU_002976"
     CDS             17797..18270
                     /locus_tag="ACHKAU_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5531998.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            18447..19571
                     /locus_tag="ACHKAU_002977"
     CDS             18447..19571
                     /locus_tag="ACHKAU_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO5531999.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            19571..20518
                     /locus_tag="ACHKAU_002978"
     CDS             19571..20518
                     /locus_tag="ACHKAU_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO5532000.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAINRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGINAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            20562..20948
                     /locus_tag="ACHKAU_002979"
     CDS             20562..20948
                     /locus_tag="ACHKAU_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO5532001.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAEAEAEQDAPRKKTGNKAEQARA"
     gene            20945..21364
                     /locus_tag="ACHKAU_002980"
     CDS             20945..21364
                     /locus_tag="ACHKAU_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO5532002.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            21361..21921
                     /locus_tag="ACHKAU_002981"
     CDS             21361..21921
                     /locus_tag="ACHKAU_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO5532003.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            21922..22167
                     /locus_tag="ACHKAU_002982"
     CDS             21922..22167
                     /locus_tag="ACHKAU_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO5532004.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            22164..23666
                     /locus_tag="ACHKAU_002983"
     CDS             22164..23666
                     /locus_tag="ACHKAU_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO5532005.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTG
                     TFGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            23675..24040
                     /locus_tag="ACHKAU_002984"
     CDS             23675..24040
                     /locus_tag="ACHKAU_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5532006.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            24055..24531
                     /locus_tag="ACHKAU_002985"
     CDS             24055..24531
                     /locus_tag="ACHKAU_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO5532007.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            24658..>26208
                     /locus_tag="ACHKAU_002986"
     CDS             24658..>26208
                     /locus_tag="ACHKAU_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5532008.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPS"
BASE COUNT         6257 a         6583 c         7586 g         5782 t
ORIGIN      
        1 gtaaccaggt gaatcatttg ataccaacga gggttttagt gaaaggaggt gaaatcacct
       61 gtaagatact gaaataaaac aggtgatgtc tggatgagcg cttaaagtct atttgatacc
      121 gattgtgcaa taattaatcg tttattctaa aatcggcaaa acttttgata ctgcgatctc
      181 aactttttaa agcgtttaaa acttcattaa atctatatgg caactaacat acgggccagc
      241 ctcgccaaac taacccgctt tagattctac cggatttgat tgcctacttg tctcggtttt
      301 ttccaaaatc tctctgacgc aatcgtctgg catatctaca atcatcaacg cagccaggcg
      361 cttcctgcct tctagttgta ataaatcctg gttttcttta tccagcagtg tcagaaccgt
      421 ctctacacct ttgcgagcaa ggagattggt taactgttct cgttcgtctt tcgtaaggaa
      481 tgaaagcatc tgtgtaagtt gatctccctt atcaccagaa caaccaaaat taccacaaat
      541 accaacatcg tttggtgatt cacccttgcc ctctttaagc cactcaagac taactccttc
      601 actttcagaa attcgtataa ggatactttc tctgggggct ggttgtgttt gtcctccctt
      661 gaaatagctg tttaatgtgt tgatattgat tccccacgct ctggcagctg cacttcttga
      721 agggtagcgc tttgtaagca ttccaatacg ttcaataaca ggttcttttc cctgcgacat
      781 aaagatacct cttggtgctt ttctaaggaa aggtttcttt ctatttatcc aattgaaaaa
      841 taaggataaa agcaaaaatt agccaaaata ccaaaaatac cttgatttgg tatttttagt
      901 gatgcattat ttacccgtgc agatatccgc acgaatatcc gcgcgggtaa ctttttagaa
      961 tagaggaagt atgagtagaa gagaagtttc agatcacgat tggccgcctg aaatcatcaa
     1021 ggcgcgctta cacatggctg gcttatcact gcgctcttta tctttaaagg ctggttacag
     1081 cagggattcg ctgaaaagcg tattacgtac tccttgccga ccgtatcagc aaataattgc
     1141 tgatgctctt ggggtatcgc ctgaagaaat ctggcccagt agataccagg ttaagagcta
     1201 tatgagaaag gcgtcatgat atgtatgtca ttgcaaaaga actgattggc gcgcccggaa
     1261 tgcctgctac aacaaaaggt attcgccagg cattacaacg ttacgtacaa gggaaaagct
     1321 gttgttcccg tcgtcgctca ggctctaaag caactgaata cagcatcgac tgtttacctg
     1381 aagtgacgca gcaggcatta cgtgaacgtt atgccctgca actgatgacg caaaaagccg
     1441 atgaatcacc ggctccggta gtgacaaagg ccaggcgctc acctgccgtg gttgatgcgg
     1501 tggaggcata tcgcggatca ccacaactga tggtcgaacg ccttaatgcc ctgactgaaa
     1561 accagcgcca ggtggctgat gcacgaatcg cgatcgtcag cgaagttctg aaagtcgcgc
     1621 aacaacctgg tttcagctgt gcgaaggcta tccggtttat cgttgacaac ctggcacgtt
     1681 cgcagctgga cgagcgcatt gtggcaatgg ttgagacggc gaacgccaaa aagggaaaca
     1741 gccgcgcgtt aagtgaaatc acgctgaaac gctggattgc ggcctttaac aaggcgcaga
     1801 acgccgctga acgcctgctt ttactggcac cgggtaagcg ccaggaaata aaagccgaag
     1861 atattaactg gctgcccgaa tttctggcgc agtatcgcca gtcaaacggc cgaccaatga
     1921 ccgaggctta cgaggatttt gtcgctgaat ggcagcaccg gcacgctgat gagccttata
     1981 tgctcgatat catgccgtct tatgacacca ttcgccgcgc aatgaagaaa ctgccggaag
     2041 tggtgaaaca aaaaggccgg gtgaccggca gtgaataccg ccagcttgag ggattcacgc
     2101 gccgcgactg gtcaaaaatg ccggtgaatt atgtctggat tggtgacggt cacggcatga
     2161 agctgaaatg cgcacacccg gttcacggtc ggccatttgc accggaagtg acctttgtta
     2221 tcgacggtgg cacgcgcttt gtggtgggct ggagccttga cctggctgaa aatgttttcg
     2281 ccgtagccgg tgccatacag cacggcattc gccatcacgg caaaccgttt ctgtattact
     2341 cggataacgg ctccggggaa accgccgaca tcctggataa ggaggttgtg gggatactgc
     2401 cgcgactggg gattaatcac ccgaccggga ttgccggtaa tccgcaggga cggggcatta
     2461 tcgaacggct taaccgcaca ttaccgatgc gcatagcccg taaataccgc acctatttcg
     2521 ggaaaggtgc agatcgcgag acgttacgca aaaccaaccg cgatttacgc tcggcattta
     2581 ctgccctgca acagggcaaa cggctgaacg cccggcagca gtcagcgatg cgtgatttac
     2641 cgtcctggtc tgaactgatt gatgccattc gtgatggtgt tgagtggtac aacaaccggc
     2701 cgcacgatga attaccggtg aagccgaacg gcaagcatta cagcccggcg gagttcagaa
     2761 aaaaacgcct ggcggaagag gacacggaaa ttgaatggct gtccgatgtg gaattgcggg
     2821 acatgttccg gccgatggtg gaacgccctg taagacgctg tgaaatacgc tggctgaata
     2881 atatttacta cgcgcccgag ctgcgtgatg agcatggccg caaggtgctt atcagctatg
     2941 acattcatga tgccgaacga attaccgtac gtcgcctgga tggcagcgtg atttgcgagg
     3001 cggtatggga cggtaataaa cgcgaagcct tcccggttag cgcggaatac tacaaacagc
     3061 agcaacgcct taaaggcatg cgtaaacgcg cagaggaaaa aatccgtgat gccgaggatg
     3121 aggttgtcaa cgtgctggag cacaagccgc aggagccctg gctggaaaac atctatcgcc
     3181 cggtaggtaa tacggtggcc gttcagcaac ctgccgttga tgatgagcct gatgaagaat
     3241 acgagcgcaa tttccagcgg ggattgcagt tgctcgaagc gaaattaaaa gaaaatgacc
     3301 cgctggcctg aaataaaaaa taacccgagc ggcgactcag gttatttgat taaacaaggt
     3361 atcaaatgag aggttaataa tatgactgat attaacgatg taatcaagac cattgatgaa
     3421 cttattgatg gcggcgtact gacgcagtat gccatcgcca gagaggcggg aatttccgac
     3481 ggcacattat cggctttccg caaggggaaa tataaaggcg ataacgccgc tgtggctgct
     3541 tccctgcgtt cctggtatga gaactggaat aaacaaagcg cactgccgga accgccgcag
     3601 tttgtggaaa cgcagacagt tcaggagctg cgcgcactgt ttcaggcggt tcgtctgatg
     3661 ggctgtatta acgttattgt gggcgtaccg ggtgtgggta aaacggccac tgcccgtaat
     3721 tactgccagg agcaaccaaa cacctggatg atcaccctgt cacccgcgca ctccagcgtc
     3781 acggagtgtc tgctggagct ggccgatgcg ctggggattg attacacccg cgcgaacaaa
     3841 ggggcattat cccgcgccat ccgccgtcgc ctgatgggaa cgcgtggact ggtgattgtg
     3901 gatgaggcgg atcatctggg tattgacggt ctggagcaac tccgggcaat tcaggacgcc
     3961 acggggatcg ggatggtgct tattggtaac ccgcgcggat tgtttaaagg tggacgccgc
     4021 gcctttgatg atttatcgcg cctgttcagc cgtcttgccc gtacaaaaca acttcgcaag
     4081 gccaaaaagg cggatgtgct ggccattgcc agggcgtggg ggatcagtgg tgaggccgag
     4141 ctggccgtca tgcaggctat cgctgaaaag ccgggagcgt tacgcgttct gacacatacg
     4201 cttaaccagg cgtggctcac cgccagcggt gaaggcgcgg cgctgacaga aaaacatatt
     4261 aatgcggcct ttaaagaggt ttataccaac cctgaattac tctcacaggt gtgattatgg
     4321 cggtatttaa tattcctgat atttacggac gcttttacct ggttaatttc gataacgtga
     4381 aggtgatttc actggccgaa aataaagaat gtggcgattt actttttgaa tttaatgacc
     4441 gcacacgaat ggtgatatct gccggacttg atcgcgaagg tgcgacagac gtttacagcg
     4501 gaatatgccg ttctgttggt gcgaaacaag tcagctaaat gaggtgttat atgaatattc
     4561 aatcctgcgg taacaaaatg aatttattcg actccctgaa cagcgcgcgc cgtctgaccg
     4621 aacttgccgg tgcggtactg gaacgcagta agcgctatcc gcaacgtttt gcactgaaaa
     4681 ccacgccgcc ggtaggcaac gtgcagggaa ccggtgaaat tgaaatcacc atacagacca
     4741 acggcctgcg tcgccgtgtg aaggccaccc gcatcagcgg ctgcacggtt tactgggagg
     4801 tatgaggtga aaaaaaatct cattgcatgg gcgtggtcga gtggtcttat tgagtttggt
     4861 tacgtcctgc cggaaggtgc attaccgata gttgccggaa agcctgccac ggtacggcat
     4921 gtgattgagg ttatggcgcg tcatggacgt gatgaacagg agcagttact ggttccgggg
     4981 ataccggaag cggtgacgga ggaagaagcc tttaatgcca tgattcggtt ctgccgtgag
     5041 gtcagacgcc gggtcagtta tccaaacaga acgaggacca gaggatgagt aaagttgtac
     5101 gcattatttt cgaatacaag gagcacgtta tccataaaaa cgcggatgga acagtgcgca
     5161 tgggggtaag tctggacata cgttcaaccg ggataaagca gaaaggtgat ggacctgcca
     5221 tgatttttgg ggtggttatg ctcgcggaaa gcagagactt tactgaactt gtggcaatga
     5281 aagccagtgc gctcatgaaa gatatggaca tgagttccgg ggttattaaa ggtaatgaat
     5341 ttaatcagca ggggtaattc catgagcaaa gtacgcgtta tttttgaatt tgaccacgta
     5401 atgcatgaag taaagccagc cggtaatgac agtgaggaaa tcacggaggg ggttactgcc
     5461 acggtaaaaa tcgaacgtga tacagagaac agaccggcag gaccctgtga tgtttatgca
     5521 caaattctta aatatcacag ccccacaatc attcagtttc tgacagatga actacagggt
     5581 tccatgcagg ccatgggggt aagcagctcc gttgaacgcc gttctgttca gaacggacca
     5641 gatacattgc aataaggaaa aacaaaatgg caaaacgcgt tacaaaatta aaggccgcag
     5701 cagaggcggc accgcagacc cgcgaagaag tcagccgcga tatccgcacc ctgggcgata
     5761 ttcagcgaga ggcgctgcgc ctggaaacag cgatgaatga cgaggtggca gaaatcaccg
     5821 cccgttatac gccgcagatt gaaaacctta aaaaagaaat caaagtgctt tttaagggga
     5881 ttcaggactg gtgcaaaacc aaccgcgatg agctgacgaa cggcggcaaa acaaaaactg
     5941 ccaatctgac caccggaacg gtgtcatggc ggctgggaac gccatcatgc agcgtcagcc
     6001 gtgatgtgga aggtgtgatt gaaatgctgc gccgtatggg tcttgagcga ttcatccgca
     6061 cgaaagagga agtgaacaag gaagccgtcc tggcggagcc ggatgcggtg aaagggattg
     6121 ccggtattaa ggtaaataaa ggcgctgaaa gtttttatgt cgagcctttt gaacaggacg
     6181 ccggactgaa caaataacat cgcattaaat ctttaaatat cacatcgttt taagtatggc
     6241 gctcgcgtca ggggactgcc tgcgcctgta aactgaaaat aaggaacagg atatatggca
     6301 tatttttatt tcaaattaga ccgtgtgcag acaaataaat atttcaccaa atatcaacag
     6361 actgttttac cgctacgcaa cagtattctt cgggcattac tgaaaaatac aggcgctgcc
     6421 ggattgcgct taaagccgtt tgccatggac gtaatcagtg agttttattt ttctggtgct
     6481 ctgcctgcgg gctggcgtaa gcgcgatgat gtggctttta tcggggacgg accgtgcttt
     6541 attgccagac ctgatgagtc atgccctgaa ggaccggcga ttgccgcaat gattgaagcc
     6601 gctgaacgcg agttaagaaa gcgtcctgat ttccttgtct ggctctgtga aaagctgggg
     6661 gtaatgagaa tcccctccat gtttaacatg gactcctggt ggaccccgtc gctctcccgt
     6721 gatgccctgt gcgtggtgtt taaagtaggc gcttatggca gggaaataaa agggggtatt
     6781 cctgaagaat gccaggagat taaacattct gaatatgtgg cgctgacgga ggaataattc
     6841 atgattgatg caaaagtgct tgaaggggtt aaaaactggc tgagtattta cgggcgtctg
     6901 acctgcggca ttctggctga aaaaatgaat atgccgccat cctcgatggt ttattttctg
     6961 cgtgatgcgg tcgatgccgg ggtgctgacg gaatgtaacg gtttttatga tattccgcgt
     7021 ccccgcccgg tgcagccggt tcgtcgcaaa tgcagccagg aaggtgcggc tgatgatgtt
     7081 cagtggtgca gtttcagaaa atccctgccg tggattgagg ggcatgatat tccgtcaatg
     7141 gcgtgggaat ttgctcaggg cgttctgacc tgtgaaaccg tttatgtggt ggctgaagtt
     7201 gatgagcagg ccatgaaaga aggcgtgccc cagtttgtga tggcgtatat cgacatacgc
     7261 ctgggtgtca ttatctgcgg tttaagcggc tggaatatca cctcgcgcgt tctgcgttac
     7321 ctgattgttg accggacggc cgcgcctgct gggatatctg cggaggtggc gtaatgttct
     7381 ttaaaacatc aaacccttcc gcgctggccg cgtggcaaaa atatcagcag gactgccaga
     7441 aagttaagga tgaggcaaaa cgccttgagg ccgtgctgaa tgttgcgtgc cggtcggtat
     7501 ttgtatccgg tatcagtggc ttttgtttta aagggctgcg ctttatggat gacaaatatc
     7561 cttttcatcg cgacttatgg cgaaaaccga ctgcgtcgaa tggctggagc tgcacaccgc
     7621 gcacatcacg tattcccaaa gccctgcgcg ttgcctctga cgaacttaac agtctgtggc
     7681 gtgaatattc gcccgtcacg tatgccagaa ccgatgcact gttgttctgg ctgggtattg
     7741 atttctcggc aatcatgttt ggccctgtga agtggttctg cgttgacgat gtgatttacc
     7801 ttgagtgcgg cgtaaaaccc gcaaatcaga aaatgaccga aattctgtct gatgagtttt
     7861 atgctgctga aaagcgagtc agggggtgat gtatgatgat tttacaaccc atggggcgaa
     7921 aaggtcaggc acctgctcat gtccgtgcgt ggacacctga agaagatgcg ctgctgattg
     7981 cgctttatcc atccaccccg gttaaggata ttgctgtcag ggtaaaaaga agtttctggg
     8041 gtgtacataa ccggattgtt ttattacgcg gtacttaccc ggagttgctc aaatgcaaac
     8101 gtccaagatt taaacatgat gaagataaat ttatccgaaa aaatgccaga acgatgaccg
     8161 gtaagcaaat cggagaatat ctggggcgag accgggattc tgtccacaat cgggcgcgat
     8221 atattggcgt aagcatgaaa aaatacggag aattgctgcc cttcacccgt atacctgacg
     8281 atgatgttca tcttattcgt gaattacggg atgctgaatc accacgacgt cttaccttcc
     8341 gggaaatagg cgagaaattt gaattatccg agagtacggt gaattttatt tatcaccatc
     8401 gccggactgc cgaggacgct gtattacggg agttaacgcc atgataaaga gacttgtatt
     8461 tttcgcaatt ttcgttccgg tgttgggggt tggttttata tttgcgatta caggaaacct
     8521 gtccatgatg ccagatattt ggttctttat caggatgtcg ctcttcctgt ttattatgaa
     8581 tcttcttatc gacatatata tccgtattac tggaaaacat aaatgagaaa aatcacgtcc
     8641 ttgtcagagt tacaggagat gaatatgagt attgaattaa gatcgtctta tgagtatcgc
     8701 aaatttctta tcgccggagg catgaaaccg gaagatgtgg aaaaaatcgt ttcttttatg
     8761 gataaggaat gtgacaagcg ggatgtgtca gaaattatta tggatgacat gattctggat
     8821 tcagtcgtgg cgttaagtcc gttatggatt gtgcattctc ttgcggaaat tgccaaaggc
     8881 accgataaac aggcagctgt tgctgccctt cagacgctta atgaaatgcg tatatctcca
     8941 cgcccgacat taatacatat gattctgtcc agcatggagg ataaagcaaa tgaatagtct
     9001 acccgctgga tgggcgaggc cactaatggc caggaagcat cattttttta aaacaggcga
     9061 aaatatcagt atatgtggac ggtggttata tctggctcat aatcgcgagc cggatacatt
     9121 tgaaagccct gatgactgtg ccgaatgccg cagaagagtg aataaggaga aagataatgg
     9181 acaatagttc tctgttgttc tggtgcttat atatcacatc ctttttcggt gcatttgtta
     9241 ttacccggtg gttatgccgg aaaattatct gcttttttga taaaagacat ccggttgaac
     9301 gggcggctga tgcgctcatt cagcaggcca ttgtgttata tagcggtgaa tttttctgcc
     9361 ggataacaac cagagatggc tggcacataa tgattattcc gccaacacac catgtccggt
     9421 gggatgaggc agaaaaggct ttccatgtcc gtaaaaaggt aaatacggta tgagaggaaa
     9481 actgatatcc gccattcatg tggcaaagcg cgagcttgcc ctggatgatg aggcttatac
     9541 attcgcgctg ctggccgcca ccggcaaaac cagctgccgg gatatgtcac cggatgagtt
     9601 atcccgcgtg ctggatgttt tcaaaaaacg cggttttaaa gtgcgccaga agccggttaa
     9661 ccgggcctta aaaccgggta cggtgaccgc caaaattcgt gccatctgga aggtgatgca
     9721 ccggcagggc tttatctctg atggtgcgga aaccgccctt aaccgctggg tgaaatcgca
     9781 gacagccgcg cagaacggcg gcgaaggtgt ggcaaactgg cagtggctgg agcaacaccc
     9841 cgccctggcc tcagatgtgc tggagcgtct caagcgatgg caccgccgca aaatgctggc
     9901 cgcgatggaa atgcccgaac gcacgctgat ggggtatgac gccgtttgca ggcagtatga
     9961 aaaatcactt ccccgttaac cccaaatccc gccacaacgc gggattttta ttttaaactt
    10021 accgggaacg cgagaaccgg aggctgatat ggcagaaact cagatgagca tgtttggtgg
    10081 tgacagtgag caactgcacg cccttatcga ccgccttgat gacatccctg atgatattct
    10141 caaaaagaac tggccgcgga ccctgtcaga actggttgaa gtcaccggcg cagaacttca
    10201 gcgtcagggg attgagccgg tactggccgg taaactggca cgcaaggtgg ctgcggctca
    10261 ggcagcctat atgggcggac ggggttatta cctgccggtc ggggaatctc tctttgccga
    10321 gctgcgaaac aatgagatat tttcgcgctg ggaccggggc gagaaaattg aatcccttcg
    10381 ccgccattac cggatgtcag aaacccagat ttataccgtc atacgcgaac agcgccgtct
    10441 gcatctggca agaacgcaac cgccactttt ctgatattcc gcaaagcctg ccgcctctcc
    10501 ttcaccgtta cgctgactca gagaacatca tgagacagcg taacaatgcc aaaactcccc
    10561 gcaccactgc gtaaaaagct gattgccctt gttctggccg gtgccgggac gttcacgatt
    10621 gccacgcatt acaccggtta ctgggaaggg aaagaaaact ccacgtatat cgatcccacc
    10681 ggtacaccca ccatctgtta cggccatacc ggcccggatg tgaaaccggg tatgaccctg
    10741 acagatgaag aatgcctgga actactggaa aaggacatga aatgggcctt tgcggccatt
    10801 gatcggcgtg ttcaggtgcc gcttacccgt ggtcagacgg tggcgctggc ttcgtggatc
    10861 ttctgggccg gtgaaacgaa ctttcgcaac tccacgcttc tgcgcctgat caatgccggg
    10921 cagatgcccg cgtcctgtaa gcagtatatc cgctggattt attcaaaggg ggtgaaactc
    10981 cccggccttg aggcccgccg ttcggcggat gaatggttat gtcgctacga cttgccgaaa
    11041 gtctgaaccg cttctggcga ccgctcatga tagcgctgct gtgtgcggta ctgctgttac
    11101 ggggtgtcct gtggctgcgg tgaataatgc cttaccggcc ctgattgccg gggcgtgcat
    11161 ggcggcgctg gggattatcg ctgtactggt tgccgctata tggggaatgc accagaagac
    11221 gcagcgcctt gaagacaata atcaggtgct tgtgcgtgag cgggatgagg cgcgtcaggt
    11281 gctggcaaat cagcagcaca ccctgcaact catttcacaa atcagtgagg cggccacgaa
    11341 tgaaaaacaa cagaacattc agcacagcga ggggcagcag agcgttgtcc gccggtcgct
    11401 ggcggcagtg cctgcggcct ctgtccttgt tcctgatgat gtggctgatc gggtgcgccg
    11461 ggccgtctgt gaaatacgtg cctgtgaagc cggtgccgat ccccgctgaa tggctggctg
    11521 actgcctggt ccctcctgcg ccggagccgt tcacgtttgg ggcatcggtc acttacaacc
    11581 tgcaactgct ggcggtgatc aagaactgca acgtggacaa ggccagtatt cgtcgtctgg
    11641 aggcgcggcg acaacatgaa tttactgata tggccggaac gcctgctgtt ccggcaggaa
    11701 agacgaagta aggaaaaagg tatggatgat tcagatcgtg ctcaggctgt gatggagcgg
    11761 ggatcagaac gcgccctttg taaccggctg acacgtaagc gccgggcaat ggatgctccg
    11821 ggcagacgtg tctgcgcgga ctgtggcgga gagatccccg ccgcacgtct tgttgctgtg
    11881 ccggatgcca tccgttgtgt gaactgccag aacatcatgg aggcccgtca tgtgggtcag
    11941 catcgttaaa gattatgttg tgccgatcct ttcggcgacg gcaaccgctg gcgggatatt
    12001 catggcgctg atgcgcaaaa cgtttgtccc ccgcgaggcc tttgaaaaac tctccgaccg
    12061 cgttgagaag gtggaaaccc gcctgtcgtc actgccgaca gaagccgagg tcaaccgtct
    12121 gaatgtggaa atcgtgaccc tgcggggtga actgaaaacc acgaacgcca cgctccgctc
    12181 tgtctcctat cagaacgaac tgctgctgga gcaggctgta aggaaaaaaa cgcaatgagt
    12241 gatttcatta ctgaagatca gcgtcttgtc attctgcggt cgctggcgga ttacaacggc
    12301 gaactgggtg aatccgtgct ccaggactgt ctggatgatt acggtcaccg cgtgtcccgc
    12361 gacaccgttc acacccacat tgcctggctt gccgagcagg gactggtgcg taagcgcacg
    12421 ctcgttaacg gttatttcat cgccgaactg accggccggg gtcaggatgt ggcggaaggt
    12481 cgcgccagcg ttccgggtgt gaagaaacca cgcgcaaggg ggtaacgatg gacaagccga
    12541 cgcgtggacg cgtgcgcaag gtggatttgc tccccgacag catccgtaag ccactgctgg
    12601 aaatgctgcg tgaaaaacgt ctgacgcagg tccagatacg cgaggaaatc aaccgtctga
    12661 tccgtgaggc gggcctgccg gaagagcagc agctttcacc ggcggccatc agccgggaag
    12721 cctcccgtaa tgagctgatt gcccgtaacc tgcgtgattt acgcgagcag acaaaagcca
    12781 tgatggccga actgggagac aagccgaccg gtgaaaccac agcgctgatt ctggagatgt
    12841 cccgcgccct gatgtaccgc cgtctgcgtg ccgcgaccga atccctgaac agtgacagcg
    12901 acgtggatat gcgtcttatt aaggacatcc tcctgtcagc acagcgtgcg gaaagtgccg
    12961 ccgagcgcag cattaagcgt gaaaaagaaa tccgggccgc atttgctgaa gagatggcaa
    13021 acgccgtcac cgacgagctg cgcggcgtgg acgggatgag tgaacagctt gagtcccgta
    13081 ttaaggggat tctgttgggt aaagcctgat gaataacgcc gaagaactct cccccttact
    13141 gacgaatacc gtctccaccc gcaaaattga tctggccggt gaaaaagccc tgctgggcgt
    13201 ggatgtgccg gacagccttg atttgcccgg tgatatgccg gtatttctgg attatcaggc
    13261 ccgctggttt gaggatgaaa gcgaggtctg catcgcggaa aaatcccgcc gcaccgggct
    13321 tacctgggcg gaagccgggc gtaacgtcat taccgccgca aagcctaagc ggcgtggtgg
    13381 ccgcaatgtg ttttatgtgg ggtcaaagca ggagatggcg ctggaatata tttctgcctg
    13441 cgcactgttc tcccgtgcct ttaaccagct ggcagatgct gatgtgtatg agcagacctt
    13501 ctgggatcgg gataaaaaag aagaaattct gacctacatg atccgctttc cgaacagtgg
    13561 attcaaaatt caggcactgt cttcccgtcc gtcaaacctg cgcggccttc agggggatgt
    13621 ggtgattgat gaagccgcgt tccacgagtc actggatgag cttctcaagg cggcaatggc
    13681 gctcaccatg tggggcgcgc gcgtgcgtat tatctccacg cataacggcg tcgataatct
    13741 gtttaatcag tacattcagg aagcccgcga ggggcgcaag gattacagcg tccaccgcat
    13801 taccctggat gatgccattg cggacgggct gtaccgtcgt atctgttatg tcaccggccg
    13861 cgaatggtca ccggaaagcg agcagaagtg gcgtgatgat ctctacaaaa acgccccgac
    13921 ccgtgaggat gccgacgagg aatacggctg tatcccgaaa aaatccggcg gtgcctatat
    13981 tcctcacgcg cttattgaaa tggcgatgct ccgcgacatc ccgattctga cgtttgaagc
    14041 cccggacgac ttcatcagcc gtgccgcatg gctgcgtgaa tcggaggttt taacctggtg
    14101 tgaagaacat ttaaaaccgc ttttagaggc gttaaatccc cgttcccgct tcagcttcgg
    14161 ggaagacttt gcccgcaccg gtgacctctc ctgctttgtg ctgctggaaa tcactgaatc
    14221 cctggcaaaa cgcgaggtgt ttcgcgtgga gctgcgtaac ctgccatatg cccagcagga
    14281 gcaggtgatg atgtacatcc tgacccgcgt tccggcgctg gtcggtgcgg cgttcgacgc
    14341 caccggtaac ggcggctatc tggccgaagc cgcgctgctg gcctttggcc cggacatcat
    14401 cgactgcgtg atgttatcgc cgaagtggta cggcgagtgg atgccaaaac tgaaggccga
    14461 gtttgaggat cagaatatcc tcgtcgcccg ccatcagacc acgcttgatg atttgcgcca
    14521 tgtgaaggtg gtgaacggta tcccgcaaat cgacaagggg cgtacgaagg accagaacgc
    14581 aacggctgtg aacgcccgcc gccatggtga ttttgccgtg gcgctctgta tggcaaaccg
    14641 ggcgtcatac atggagggct tcatcctgga tgaatcagcc tgtcaggcgc tgccggagcg
    14701 gtcgcgggca atggagggcg gttatcgtga tgacgatgag gcatatcatg aatttgatcg
    14761 ggggtgctgg tagtgggacg cataattgat cttgacggaa aacctttctc ctttgaccca
    14821 gagatgcaga gtgccgtgct ggatattccg cagattgcca gccgttatat tgaacatccg
    14881 gcctcgggta tcaccccgaa ccgggcggca cagtgccttc gcggggctga acgtggcgat
    14941 ctgattgccc agtccgatct ggccgctgac attgaagaaa aggataccca cctttttgca
    15001 gagctgggca agcgacgtct ttccattcag ggcgtgccct ggagtattga gccgccaccg
    15061 aacgccagcg cgaatgagaa aaaggacgcg gaaatgctcg acgaatatct gcattccgcc
    15121 gactggtttg atgccatgct gtttgatgcc accgatgcca tcctgaaggg ctattcctgc
    15181 atggagattg agcacgggat gctcggtaaa atgcacatca tccgcgccat ccgctggcgt
    15241 gacagcgggc atttctgcct aaacccggat gatttgagcg aactgcggct gcgtgacggc
    15301 agccatgccg gggtggcgtt tcagcccttt ggctggatag tgcatcagtc acgttcacgc
    15361 accggctacg gtggtgcgac ggggcttgtc agaacgctta tctggccgtt cattttcaaa
    15421 aactattccg tgcgcgatct ggctgaattt ctggaggtgt acggcctgcc gatgaaggtc
    15481 ggtaaatacc cgtccggggc aacgccggag cagaaaagcg ccctgatgcg ggcggtgatg
    15541 gatatcgggc gacgtacagg cgggatcatc ccggccggga tgtctctgga gtttcaggca
    15601 gccgcgaacg gtcaggccga tccgtttgaa accatgattt cgtgggggga gcgttccatc
    15661 tccaaagcta tcctcggcgg cacgctgacc acggaagccg gagacaaggg cgcgcgctcg
    15721 ctgggtgaag tgcataacga ggtgcgccgg gaaatcaggg attctgattt acgtcagctg
    15781 gccgccacgc tgaaccgcga tctggtgtat ccgctgtatg ccctgaacac cgcccacgcc
    15841 atagatatcc gtcgcctgcc gcgtatctgt ttccagacaa aagagccggg ggatatcacc
    15901 aaaatcacca gtgcggtgat gcagctcagt acggggatgg atattcctga tccctgggta
    15961 cgggaccaga ccggtattcc acagcctgcc cccggtgaag ccatcttccg tgtccggcag
    16021 agtggcaatg aacctgctca gacagacaaa gagatgccac cgggaaaaca ggagaagaca
    16081 gagcagacag cgctggctgc cagactgccg gaagcaaaaa gcacgccccg tgatgaactg
    16141 gacgacatgg gggatgcggt gcctgcccgc ctgttacagg aggccataga cccggtgctt
    16201 gagccggtca ttgatgccat cagaacgcgg gggctggcgg aggcactggc agacctgcct
    16261 gccctttacc gtgaaatgga tgattcccgc ctgatgacgc tgctcagtga tgccatgttt
    16321 gctgcggaaa tgaaggggat gctggatggc acaggggatt gatttaggtt atgccgccac
    16381 acttgcctca aaagaggcgg tggcatactt ccgcgccaaa ggggcgcata tcagctggaa
    16441 ctggttcgaa acagacgcgg atgttcatgc ccgttcattc acggcggcaa aagcggcacg
    16501 cctggacgta ctgactacac tacaggcgga agtgcaacgg gctattgatg aggggatttc
    16561 acagaaagca tttatccgca cactgacccc ccgcctgcaa aagctgggat ggtggggaaa
    16621 gcagattgtg gtggacagcg ctggtaacgc ggaagaagtg cagctgggta atccccgccg
    16681 tctggcgctg atttacaacg tgaacacccg cgtggcttac aatgccgggc gttacacgca
    16741 gatgatgaac aacacggaca cgcatccgtt ctggcagtat gtggcggtga tggacagccg
    16801 cacccgcccg gcgcattccg cccttaacgg gctggtattc cgctatgacg atccgttctg
    16861 gaatacacac tacccgccca atggctggaa ctgtcgctgt cgtgtccggc cattgtctca
    16921 ggcccgtctg gatgcgatgg ggctgtctgt ttcatccggt caggatcatc tctccacccg
    16981 caatgttgag gctggcgtgg ataagcagac cggagaagtc agagaaatgc cggtgaccac
    17041 atattcagat ggcaccagaa ccatgacacc ggatgtgggc tggtcatata accccggttc
    17101 ggcggcgttc ggcacagacc aggcgctgat ccgtaaactg atcgaggtga aaagcccggc
    17161 gctgcgggaa atggtggttc aggagatgaa caacagcccg gagcggcaac tggcgttccg
    17221 catctgggca aaaaacatca tgaaaacccg gcgaggcggt aacgatatcc gcacgctggg
    17281 ctttatgacc gaaagtattg cgcaggcggt ggaaagccgg acgggaacgc caccggcccg
    17341 cctgctggcg atgagcggta aaaatgtgct ccatgcggac agcatgaaac atcagaatga
    17401 cggtatcgcc ctgacgccgg aggatttcgc gcagcttccc gccatgctgg ctgcccccga
    17461 tgccgtgctg tgggatcatg tccatcagaa cttgctttac atcactgaaa cccgtgacgg
    17521 aacggcaaag attgccgtca atgcgcctta tagtgtgaaa cgtcagccgg ataagctgga
    17581 tgtggtgatt aatgcctata gagtgagcag gaatactctg attgatgctg ttcagggagg
    17641 taagatggag atcctcgaag ggaaattgta agtcggtggt gggggtcgaa cccacataaa
    17701 catccttcgg gtgaagatat ccctcaggtg ccgctttacc agttaagagt acaccgacct
    17761 gcttttattt taaccaggta gtttcacgga ggcaatatgt catccattga tgccgctgtc
    17821 gttgttgacg ttacgcgtct ccagcgggtc tttgcccggc ttcagtttgt gggtggtgga
    17881 aaagacctgg cccgcagtgt ggcggcgagt cttctgtcat cgtcagaaat ggcgtttgaa
    17941 caggaaaaag agccggacgg cgaacgctgg catgactggt cagatcctta ccgcaagtgg
    18001 cgtacccgta agggatacat gcccggcaaa atcctgacgc tgaacggcga tctggcccga
    18061 cgcctgacca cggattatgg cgacacctgg gcgctgattg gatcaaatga gccttatgcg
    18121 gccattcatc agtggggtgg cctgcccggt atgccgccgg gaccggcggc cattggtgca
    18181 cgtccgtata tgggctttga tcaggtggca gagcaggaga tcatggacga aatcagaaaa
    18241 cgctttaaaa aggccacaga aacgccttaa ttgtttaagg tatgcaaatg cattacctta
    18301 cccccttcag gcgcgtgtcg tgattttcta acctgaattt aacgggcttt aaaatctgcg
    18361 tgaagcgcct ttgtattttt cccctgaaag cacttcctga aatcccgcaa aacccgccct
    18421 gaaaattccc ggcctatgct gccggaatga agacgaaaaa cacgcccaaa cttgcttatg
    18481 ccattctgaa tgccatcagc ctgtccgcag acggggacgg tgactggtgt cagatcatgc
    18541 cagccggtcg ggtaaaggcc cgtgacggtc gcccggaaaa accggccgag ggctggctga
    18601 tcaaccatgc agccgttgag cgcatggtct cccgtgttgt ggcgctcaat cagccggtga
    18661 aaattgacta caaccaccag accctgattg agggacatcc ggcaccggct gccggttttg
    18721 tgatggcctc accggagaat tttcgcttca gtgaagagcg aggtttcgag gtgcgcccga
    18781 agtggaaccc tccggccctt gaacatctgc gtaataacga attcccctgg ttttcaccgg
    18841 tgattggcta tgacgagagc accggcgaac ctgtcgaact tcggatgctg gctattaccg
    18901 gtgaccccgg tctgaccggc atgaatcctg tcgccgcact gtcggcggat gacctttata
    18961 acgccttaaa ccctccttta aaggatacct ccatgaatga gcaattacgc cagttgctga
    19021 cggcgctcgg tctgaccgtg gctgacggtg acgaatttac gccggagctg ggcacggcag
    19081 cgctgtctgc cctcaccggg atcaaaaccc gtgcggatgc acacgacaac ctgaaaacac
    19141 aggtcgccag tctgtcggca gagctggaaa ctgcaaaagg cacaccggct ggcggcagta
    19201 ttgacctgac gaaatacgtg cccgttgaaa cgtataacgc cctgcgtacc gaatacgttg
    19261 cgctgtcggc gcagcacggc agcaccacgc tggagcagtt gctggacaag gccgagtccg
    19321 aaggacgcat cttcaaaagt gaacgcggct atctggaaca actgggcggg cagattggcg
    19381 tggcggcact ttctgcacag cttgacgccc gccagccggt tgcggccctg accggccttc
    19441 agaccgacac cgtgaccgtg ccggataaaa agactgccac cgctgtgctg tcggctgaag
    19501 atatcgcggc cgccaaactg ctgggtaaaa ccgaagccga gttcctgaaa atgaaagagg
    19561 aaatgcaata atgcctaccc cgattacacc ggcgatgatc accgccctga tgacgggcta
    19621 ccgttctgat tttcaggccg ggatgtccat ggccccgtcg cagtacaaaa aaattgcgat
    19681 gaccgtgccc tccacatcga aatccaacac ctacggctgg ctggggcagt tcccgcagtt
    19741 ccgtgagtgg atcggctccc gcgtcatcga gaagatgaag gcctatggct atgccatcgt
    19801 gaacaaaacc tttgaaggca ccgtcgccat taaccgcgat gacttcgaag acgataacct
    19861 cggtatttat tccccgttgt tccaggagat ggggcgcgct gctgcggcac aaccggatga
    19921 actggtcttt gctgctcttc gtgacgggat taatgcggcc tgctatgacg gccagaactt
    19981 ctttgatacc gaacatcctg tttaccccaa agtggatggc tccggggatg cgcagatggt
    20041 cagcaatatg tttgtggcaa aaaccggctc tgtcggtgca caggcggatt acagcggtcc
    20101 tgcctggtat ctgctcgact gttcacgcgc aatcaaaccg ctgatttatc aggatcgccg
    20161 taaggctgaa ctggttgccc agaccaaagt cgatgaaggt cgcgcattca ccgataacga
    20221 atttgtgttc ggtgcttccg cccgtcgcaa tgtgggctac ggcttctggc agatggccta
    20281 catgatgcag tcaccgctga cgctggatgc actgtggcac ggctggtcag ccatgcgcga
    20341 atttaccgct gacggtggcc gcaaactcgg cattaaaccc acccatattg ttgtccccac
    20401 ctcgctggaa aaacaggcgg tgcagttgct ggagcgtgaa ctgttcgcag acggaaacgc
    20461 aaccgtctct aacgagatga agggcaagct ggagctggtt gtcgcggatt atctgtaaac
    20521 ggtgagccgg gtttacggcc cggcccctgc ggagggcaaa tatgaatgaa catcacactg
    20581 tggcagcaac agatgattca ggtcttcagg tcagtggtga caattctgtc acggtgctgg
    20641 cagaagtgcg ttgcagtcgt tcggcgtttc gtcgtgcggg gttcctgttc acgcgtggac
    20701 gtcagcaggt tgaggtcacc ccggagcagc ttgcccgact ggaggaggag ccatgcctca
    20761 ctgtgcgaat actacagacg tctgctgatg atgcggggag cgtggcgggt gtggttcatg
    20821 cagtggccgg tacagattta gccgaagccg aagccgaagc cgaagccgaa gccgaagccg
    20881 aagccgaagc cgaacaggat gctccccgga aaaagaccgg taacaaagcg gaacaggccc
    20941 gtgcatgaat tacgccactg aaaccgatat gcgggcgcgt taccgcgagg atttgctcag
    21001 gccgttactg gctgtgcccc gttcggatga gccggacacg cgcaagctga accgggcgct
    21061 gacagatgcg tcagccctta tcgacagcta tctgtccgcc cgttacacgt tgccgctgga
    21121 ggttattccg gccgttcttg ttcagcactg ttgcgcgatt gccttttatt acctgtgcga
    21181 tcagcgagcc tccgatcagg cgcgtgaccg ttaccgtgaa gcgctggcct ggctgaaaga
    21241 tgtcatgaac ggcaatgtgc cggtcggtgt ggatacgaac ggtgcggccc ctgaatccgg
    21301 ggatttacca caggttcagt ctgatgcagc ggtattcggg cgcaaccaga agggcttcat
    21361 atgattacgg aaaccgaaca ggcatacatc gcccgtatcc gtgagtattt cgggaatgaa
    21421 ctggtgtctg ttgacacgca tcccggcgac tggagcgaca gcgtactgcg caccatgctg
    21481 attaacgccc cggcgatcta cgttgcctgg ctgggtgccg gtgaaggccg tacccgtggt
    21541 cgcctggtca gtcactgggt gttctacgtc atcggcgaca tgctcaacgg gcgtgaggcc
    21601 agccgtcccg gactgtatca gattgtggcc cggctgattg ccgtgcttaa cggcttcaga
    21661 acagaaaaaa cctcaccgct ttactttgaa aaggcggtca acggttacac cgaaacccag
    21721 gcggacagtg gtgcggtgat gtatgcgctt tatttctcct gcgaggaaat gatcgcaccg
    21781 ctgaccgata ccagctcgct ggacgacttc ctgcgtcatt acgaaacctt tgtggagccg
    21841 cccggtacgc cgccgtttga ggcacatatt gatttgccgg ggaacacgac aacggcggtc
    21901 gaaccgccgg aggaactgta aatgaaaacc atctttatca aacccgcgcc gggacgcctg
    21961 attcgtgatc ccgacacaat gcgcccgctg gcacaggagg gggaggaaaa gccctttacg
    22021 ccgttctggt gtcggcgtct tgatgacggc gatgtcattc aggctgaaaa agccgctgaa
    22081 gaggctccgg ctgtatctgc ggatgcaacg actgcgacag aaaaaccggt cgcccaaccg
    22141 gcatccgata aggagaaacc gcaatgatta attttgacgg gatcggtaat gaccgccgta
    22201 taccgctgat tgaggttgag tttaataact ccatggctgt caccggcact ccggcacagc
    22261 gtcagtgtgt gctgctgttt ggtcaggcga gaatgaaaga gaatgccgtg gacggtgccg
    22321 gtgttctgga tgttccggtc aggatcaccc gtgcgtctca ggcaacagaa ctctgggggc
    22381 gcggctccat gattgccctg atggtggcgg aatttatcgc catcaatcct gatgctgaac
    22441 tgtatgccat tgctcagggg aacggcaccg gacaggcaac ggctgcggcc atgaatctga
    22501 ccggaacggt cacccgtgac gggattgttt atgcctgcgt gggggggcgg cgttatacac
    22561 tcccggcacc aaaaggtaag aaaggcaaag aactgaccga cgagctggcc gcgctgatca
    22621 atgccgatcc tgacgcgccc ttcacggcct cctctggtgc aggaagtggt gataatggcg
    22681 caggtctgaa agggtctctg gggatcaccg cccgctttac cggtgaatgc tccgttcatg
    22741 atgtgcgcct gaactatcac gacggggaag caaccccgga aggaatacag gtcgctatag
    22801 cctatccgaa acaaaaagcg gctaacccgg atatcacccg aagcgtggcg ggtatgggcg
    22861 atcgccagta caactatgtc gtcatgccct ataaggatga tgcaaacctg aaaatcatca
    22921 gtgatgagct tctgaaacgc tggggaccgg ccaaaatgtc tgacggtgtg ctgtggatgg
    22981 cgcataccgg cacgtttggt gaggtgcagg cattcggggc aaaacgcaat gattttctgg
    23041 cgacctgcac attcattccc aaagcccctg aaccggatta cctctgggca gcggcggtct
    23101 gcgccacctg tgcgccgtct ctgggggcag atcctgcgcg tccgcttcag acgctggcgc
    23161 ttccgtcacg catggccccg gccccggccg atcgtctgac gcgtgaagag cgtaacagtc
    23221 tgctgtgggg agggattgcc acggtcagtg tggctgccgg tgatgtggtt cagatcgaac
    23281 gtcaggtgac catgtatcgc cagaacgtgt acggcgaaag tgatcccagc tatctggatg
    23341 tggaaaccat ctacaccctc tcctggctgc gttattccct gcgtactttt atcacacaac
    23401 gttttccgcg ccataaactg gctgatgacg ggacgcccgt tcgtgccggt cagaatatcg
    23461 tgacgccgga gatcatgaag cttcagttaa tttcgctggg tgaagagtgg gtggaactcg
    23521 ggctggtcga aaatctggac acctttaaaa agaatctgct tgtggagcgt aatgcgtcag
    23581 atcgaaaccg tcttgacgtg ttatgcacgc cggatctggt taaccagttc cgcttcctgg
    23641 cagcacagat ccgtttcatt ttgtgaggta aggcatgagc ggaaaacagt atcagggcac
    23701 ggccactatc cgtgtgaacg gtcaggaata cgagacgctt gagggggcta cattttctcc
    23761 gtcaggtttt gaacgtgaag tggtgaaagg cgcgaaagtc tatggttacc gccagaaacc
    23821 acgtgaagcg acactggact gcaaatttcc ggcaggcgga gaaggttcac cagccgccga
    23881 tgaaatcaac aactggactg ctgtcactat cgagtttgtg gcggataccg gcgaagtcca
    23941 catgatgacg aaggcctgga gcagtgaacc ggcctcgctt gacggtggcg gggagatctc
    24001 cgtgaagttc gccagcgcca ccagtacccg tgttcagtga tcaggaaaaa taaaatgacc
    24061 acacgtaaga aaaaaacggc agtttccgag gcagccgtga tggaggcaat ccgggaagca
    24121 cttgaggggg ctgatccacg cactgccggg ctgacagagc aactggcaaa gggatatgtg
    24181 gatctgcttg atggtctgcc gtttggtgag acccgtgaat atcgtgtcac gttccgggaa
    24241 ctgacagcga aagacagtat tgacgcagaa gcagaagccg agcgcgtggt ggagacaaac
    24301 aatggcccga tgctgatagc gtctccgtcg ttacgcggtg ttgcactgct gcgccgtcag
    24361 attgccgcag tgggagacat tgaaggtccg ttgtcacccc gtcagattgg acagttaagc
    24421 gagcgcgatc tctcccgcct gatggcagcg gtcagcctgc tggataccgc gcttgccgga
    24481 aagctggcag ctgaccgggg gcgatcaggc gcagtgtcgg gatcagattg aagaagcggc
    24541 aattatcctg gggatggtga caaaaagcgg tccggaatgg gcgcttaacc tccccctgtc
    24601 gcaactttac cggcactgcc gacagacaga aaaaatcatc agaacgaagc agtaagtatg
    24661 gcccgaaatc tcagagcatc tctgatagtt gatttgctcg gcaatatctc agccaaatcc
    24721 cgccagtggt cacaggagct gggggcattc tcacgctccg gccaggcggg gctgggtggc
    24781 ctgggaaatg ctgcccgccg tgccgggcag gaaacagatg tactcggaag caggatgcag
    24841 cgcacgctgg ccggggtgcg gggcagtatc cgccatgtca cttctgattt tgaccgtctt
    24901 cagggcagta ttaccggcac catcgggcgg atcagcaatc tttacggaat gctggccggt
    24961 ggtgctgctg tatatggctt taaccgggga tttattcgcc cggcagcgga gatggaaacc
    25021 tatatgattc gcctgaactc cctttacaaa ggggatcggg cgaaaactga tgatgtccgc
    25081 aggtgggcaa tacagaatgc gaaggaaacc acctgggggc tggctggcgt catgcaggaa
    25141 tatacgtcca gtctgggctt tggtatgagt gacagggagg cccgaaattt tgtcaccatg
    25201 cttcaggatc agggtgccgt gggcggctgg tcactgtccg atgcacaggg ggcatccctg
    25261 caactgaaac agatgtatgc aagaggcagt attcaggcag cggatgcaaa tattcttgcc
    25321 acctacggca ttaatgccta ccgcgtgctg gctgatcgtc tgggtgtgga ccagaaagtg
    25381 gtcagaaaac ttggtgaaaa agggctgctt gggccggaca gtattcgcct gttatttcag
    25441 acgctggcgg aacaggccag gggagcacaa aaggacgcaa tgaattcctg gagtggcctg
    25501 acggccatga tgggtgacgt ctgggatcag tttgcccggg atgtgatgga cagtgggcca
    25561 tttgaaaaac tgaagggtaa tctcagggga tttctttcat gggttgacag tgccaaatct
    25621 gatggcagtt accattccct ggcggaaagt acggcatcag ccatgaacca gggctttgag
    25681 tatgcccgtg atgcagtgac agggttttat caggcgatca gaaaggtacg cgacacgctt
    25741 caggctctgc gggatgcggg ctatggtgat gcgctggatc gcattggtca gggggcacag
    25801 accgccgcga aatacctgct gtacatgtat ctcgccaccc gtgccctgaa aatggcgcgg
    25861 gctgtcggaa cggggattat acgtccgggt gctgcattgc tgggatacgg attatcagcg
    25921 gcggccttcc ttacctcacc tttccggcgc tctcctccgg ccggaacacc accaggcaca
    25981 tctcagggac gagggcaacg ttttataaac tttcttaccg gggtaaatcc ggcagccgtt
    26041 cagccagtac ttgtaacaaa ctggcccaca ggaggactgt ccggcaccgg cgcgaccgat
    26101 acatcctcag gcggacgtca ggggcggggc cgcaggaaac gcggaccggg gcgcggaaga
    26161 ccggtcacgc ctcccctgcc accgtcccca gtgccaccgt ctccctct
//
LOCUS       DAZFJF010000069        26169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFJF010000069 DAZFJF010000000
VERSION     DAZFJF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.069"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..882
                     /locus_tag="ACHKAU_002987"
     CDS             <1..882
                     /locus_tag="ACHKAU_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5532009.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     KGISPFEYANVNNPLRR"
     gene            1173..1526
                     /locus_tag="ACHKAU_002988"
                     /pseudo
     CDS             1173..1526
                     /locus_tag="ACHKAU_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1787..1969)
                     /locus_tag="ACHKAU_002989"
     CDS             complement(1787..1969)
                     /locus_tag="ACHKAU_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532010.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICFLQKVMIIDKTKYK"
     gene            1908..3998
                     /gene="iha"
                     /locus_tag="ACHKAU_002990"
     CDS             1908..3998
                     /gene="iha"
                     /locus_tag="ACHKAU_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO5532011.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(4216..4375)
                     /locus_tag="ACHKAU_002991"
                     /pseudo
     CDS             complement(4216..4375)
                     /locus_tag="ACHKAU_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(4578..4898)
                     /locus_tag="ACHKAU_002992"
                     /pseudo
     CDS             complement(4578..4898)
                     /locus_tag="ACHKAU_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(5322..5897)
                     /locus_tag="ACHKAU_002993"
     CDS             complement(5322..5897)
                     /locus_tag="ACHKAU_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5532012.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(5966..6544)
                     /gene="terD"
                     /locus_tag="ACHKAU_002994"
     CDS             complement(5966..6544)
                     /gene="terD"
                     /locus_tag="ACHKAU_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO5532013.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(6593..7633)
                     /gene="terC"
                     /locus_tag="ACHKAU_002995"
     CDS             complement(6593..7633)
                     /gene="terC"
                     /locus_tag="ACHKAU_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO5532014.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(7656..8111)
                     /locus_tag="ACHKAU_002996"
     CDS             complement(7656..8111)
                     /locus_tag="ACHKAU_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5532015.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(8134..9291)
                     /locus_tag="ACHKAU_002997"
     CDS             complement(8134..9291)
                     /locus_tag="ACHKAU_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5532016.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(9291..9872)
                     /locus_tag="ACHKAU_002998"
     CDS             complement(9291..9872)
                     /locus_tag="ACHKAU_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5532017.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            10195..11253
                     /locus_tag="ACHKAU_002999"
     CDS             10195..11253
                     /locus_tag="ACHKAU_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO5532018.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            11263..12405
                     /locus_tag="ACHKAU_003000"
     CDS             11263..12405
                     /locus_tag="ACHKAU_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO5532019.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            12398..13171
                     /locus_tag="ACHKAU_003001"
     CDS             12398..13171
                     /locus_tag="ACHKAU_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532020.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            13173..14252
                     /locus_tag="ACHKAU_003002"
     CDS             13173..14252
                     /locus_tag="ACHKAU_003002"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO5532021.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            14252..15208
                     /locus_tag="ACHKAU_003003"
     CDS             14252..15208
                     /locus_tag="ACHKAU_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO5532022.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            15219..16427
                     /locus_tag="ACHKAU_003004"
     CDS             15219..16427
                     /locus_tag="ACHKAU_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO5532023.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            16445..16912
                     /gene="terW"
                     /locus_tag="ACHKAU_003005"
     CDS             16445..16912
                     /gene="terW"
                     /locus_tag="ACHKAU_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO5532024.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            17173..17502
                     /locus_tag="ACHKAU_003006"
     CDS             17173..17502
                     /locus_tag="ACHKAU_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5532025.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            17489..17869
                     /gene="tnpB"
                     /locus_tag="ACHKAU_003007"
     CDS             17489..17869
                     /gene="tnpB"
                     /locus_tag="ACHKAU_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5532026.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            17912..18742
                     /locus_tag="ACHKAU_003008"
                     /pseudo
     CDS             17912..18742
                     /locus_tag="ACHKAU_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(18773..20386)
                     /locus_tag="ACHKAU_003009"
     CDS             complement(18773..20386)
                     /locus_tag="ACHKAU_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO5532027.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(20417..20767)
                     /gene="tnpB"
                     /locus_tag="ACHKAU_003010"
     CDS             complement(20417..20767)
                     /gene="tnpB"
                     /locus_tag="ACHKAU_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5532028.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(20764..21189)
                     /locus_tag="ACHKAU_003011"
     CDS             complement(20764..21189)
                     /locus_tag="ACHKAU_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5532029.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            21243..21994
                     /locus_tag="ACHKAU_003012"
                     /pseudo
     CDS             21243..21994
                     /locus_tag="ACHKAU_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            23377..24048
                     /locus_tag="ACHKAU_003013"
     CDS             23377..24048
                     /locus_tag="ACHKAU_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532030.1"
                     /translation="MEFSVIFFWVITGLITILFLKSIITDLIKNLNKTDIALYILAFL
                     SAGYTFQYIYNDNIFSELWGVGLFVTLIVLNIVFNLFFRLLFILSKMILMSRHIRKMQ
                     KKLKQTSVPDFIYYSIEKHNKYPIYVLHMPSNNIIEIGYNIINTDLVIGEKIHFRTLS
                     NRSLYFNLTQPPLIATIKDDVFCTLHDYYNHNNETKSTIDNYISKIKDNHNTPWLLNN
                     ENVQE"
     gene            complement(24919..25059)
                     /locus_tag="ACHKAU_003014"
     CDS             complement(24919..25059)
                     /locus_tag="ACHKAU_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5532031.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(25361..25624)
                     /locus_tag="ACHKAU_003015"
     CDS             complement(25361..25624)
                     /locus_tag="ACHKAU_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5532032.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            26047..>26169
                     /locus_tag="ACHKAU_003016"
     CDS             26047..>26169
                     /locus_tag="ACHKAU_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO5532033.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSE"
BASE COUNT         6700 a         6615 c         6445 g         6409 t
ORIGIN      
        1 ttccccttta tcggcaaccg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagcaaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgctgtaa tgcgttacgc cgtacagtct ggcatcatca gatataaccc ggctctggat
      181 atggctggtg cattgaccac ggtaaaacgc cagcatcgcc ccgctctgaa tctttcacgc
      241 ctgcctgaac ttctgtcgcg tattgacggt tataaaggcc agcctgtcac ccggcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatatgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg ctccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggctgtag ccatactgac agaactacag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccgcg taaaccaatc agtgaaaata ctgtaaataa agccctgagg
      601 gtgatggggt atgacacaac ccaggatgtc tgtggccatg gattccgggc gatggcgtgc
      661 agtgcattga ttgaatcagg tttgtggtcc cgcgatgctg tggaacgtca gatgagccat
      721 caggagcgta atggtgtacg tgctgcgtat atccataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt cttgatgcga accgggagaa ggggatcagt
      841 ccgtttgaat acgcaaatgt taacaatccg ctgagaaggt gacagaaggc tattcacaca
      901 catgctcttc ccatggggaa gagcaacctg ctccaataaa tttctttttt attccggctg
      961 atagcaccgc aatcacccgt tacctctaaa tagccactta gcattcggcg tataagttga
     1021 cagagaagga gtatctcagc aggagcttaa agtgtggttt taggcagaat ttgattgatc
     1081 ctccatttgg ggcatgtgac taatcgatat agcttcctga acggaaaaag tgttttccct
     1141 tccaatataa actattcgac aataatataa atcaattaca gaataaaact ctgtataaac
     1201 atgcaaaaat ggtaaaggcg ttcgactata tcctgaatca ctggaatgcg ctgaacgagt
     1261 tctgtcgtga cggctgggtg gaaatagaca acaacatcgg tgaaaacgcg ttacgatcgg
     1321 tggcggttgg aagaaaaaat tatctcttct tcggctcaga caagggggga gaaagcgcgg
     1381 cgatcatcta cagtctgctg gtcacctgca aactgaacga agtggagccg gaggactggt
     1441 tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgtgtgcat gaactgctgc
     1501 cctggaactg ctcaactgta aaataatcct tacgctacgt acttctcggg gcgcttacga
     1561 atttttcacg tgccacagaa ttttgaacgc tttctcttac aatatttcaa tgtttctatc
     1621 agtattcgcc ggaagaagtc atcgaccaaa tcatcccagt cgtctcgcac cactgaccat
     1681 tcatggttga catgtggtgg aaatccgctt ctacagtaac cattttttat tcgcaaaacc
     1741 gaacacgcca tacggatagc tgttaactgg catgccgagg cagtcgttat ttatatttag
     1801 ttttgtcaat aatcattact ttttgcaaaa agcaaatata aattattctc attattgttt
     1861 gtatttgtgt attgtcttgc cggttaacat gatcggagat tagtaatatg cgaataacca
     1921 ctctggcttc cgtagtcatt ccctgtctcg gattttcagc cagcagcata gctgctgcag
     1981 aggatgtgat gattgtctcg gcatccggct atgagaaaaa gctgactaac gcagccgcca
     2041 gtgtttctgt gattagccaa gaggaattgc agtccagcca gtaccacgat ctggcggagg
     2101 ctctgagatc agtagagggt gtggatgttg aaagtggtac gggtaaaacc ggtgggctgg
     2161 aaatcagcat ccgaggaatg ccagccagtt acacgctgat actgattgat ggtgttcgtc
     2221 agggcggaag cagtgacgtg actcccaacg gtttttctgc catgaatacc gggttcatgc
     2281 cccctctggc cgccattgag cgtattgagg ttatcagggg gccgatgtcc acactgtatg
     2341 gctctgatgc gatgggcggt gtggtgaata tcattaccag aaagaatgca gacaaatggc
     2401 tctcttccgt caatgcaggg ctgaatctgc aggaaagcaa caaatggggt aacagcagcc
     2461 agtttaattt ctggagcagt ggtccccttg tggatgattc tgtcagcctg caggtacgcg
     2521 gtagcacaca acagcgtcag ggttcatcgg tcacatcact gagcgataca gcagccacgc
     2581 gtattcctta tcccacggag tcacagaatt ataatcttgg tgcacgtctt gactggaagg
     2641 cgtcggagca ggatgtgctc tggtttgata tggataccac ccggcagcgt tatgataacc
     2701 gggatgggca actggggagt ctgacggggg gatatgaccg gaccctgcgc tatgagcgaa
     2761 acaaaatttc agctggctat gatcatactt tcaccttcgg aacatggaaa tcgtatctga
     2821 actggaacga gacagaaaat aaaggtcgtg agcttgtacg cagtgtactg aagcgcgaca
     2881 aatgggggct tgccggtcag ccgcgggagc ttaaggaatc gaaccttatc ctgaattcat
     2941 tactgcttac ccctctggga gaatctcatc tggttacggt ggggggcgag tttcagagct
     3001 cgtccatgaa agacggagtt gtccttgcca gcacaggtga aactttccgg cagaaaagct
     3061 ggtcggtatt tgctgaggat gagtggcatc tcacggatgc acttgcgctg actgcgggca
     3121 gccgctatga acatcatgag caattcgggg gacacttcag tccgcgtgca tatctggtct
     3181 gggatgtggc agatgcctgg acgctgaaag gcggtgtgac cacgggatat aaggcaccca
     3241 gaatggggca gctacataaa gggattagtg gtgtgtccgg gcagggaaaa acaaatctac
     3301 ttggtaaccc cgacctgaag ccggaagaga gcgtcagtta tgaggctggg gtgtattacg
     3361 ataaccccgc cggtctgaat gccaatgtca caggttttat gactgacttc tccaacaaga
     3421 ttgtctctta ttccataaat gataacacca atagctatgt aaacagcgga aaggcccggt
     3481 tgcacggtgt ggaatttgcc ggcacattgc cgctgtggtc agaggatgtc acgctgtcac
     3541 tgaattacac ctggacccga agtgaacaac gtgatggtga taacaaaggt gcgccgctga
     3601 gttatacccc tgaacacatg gtgaatgcga aactgaactg gcagatcacc gaagaggtgg
     3661 catcatggct gggtgcccgt tatcgcggga aaacaccacg tttcacccag aattattcgt
     3721 cactgagcgc tgtacagaag aaagtgtatg atgagaaagg agaatacctg aaagcctgga
     3781 cggtggtgga tgcaggtctg tcgtggaaga tgacggatgc cctgacgctg aatgctgcgg
     3841 tgaataacct gctcaacaag gattacagtg acgtgagcct gtacagtgcc ggtaagagta
     3901 cgctgtatgc cggtgattac ttccagacgg gatcatcaac aacaggatat gtgatacctg
     3961 agcgaaatta ctggatgtcg ctgaactatc agttctgata ataacaaaac gctatcactg
     4021 acggtagaat acgttgccac tgcaactcct ggcggaacag tggcaacgta ttaggttaag
     4081 tgcatttccg atccgctaat gagatttcgt taccaacaac taatatcgtc acaggaaatg
     4141 cacggattat ttttaactta tcatttacat acttgtccag agtgtaagcg caccgtgaag
     4201 gacgtggggt aaaaattagt ttacagagag agtgacgttc cagggcaaca actctttcat
     4261 gcggttggca ggccaggtgt tgattacact gatcacgtgg cgtagccacg tttccggctc
     4321 gattccgtta agttttgcag ctaccgatca ggctgtacat cactgccgca ctatcgctcg
     4381 tcatctcaaa gtccggtctc gtcagcagga aggtatcatt ctctcccgcc atttttccag
     4441 gggcccggtc agataagtcc ctttgtctat cgctgactcc tgactcataa cccggttagc
     4501 agaatgcagg gtcaccacct cgccacgacc aaatccaaat aagtcaattg caccttctca
     4561 atcgccattt tgtcagtaag cgtacagcct gaactgatgg tatcttcacc atcaatgaca
     4621 acggtgatcg caattttact gacgttcgcc ggaacacgat ccagtgctat ctcaatgctg
     4681 gcctgctgcg aaccggtaac gagcctgact gccccctcag gagaagacaa attattataa
     4741 aagataaagt cagaatcgcc actgaccttt ccctgagcat taagcatgaa caggcaggta
     4801 tcgggttcgc ctttaaagcc ggatttggct gggtactgaa gattcagcct gatcgtagat
     4861 tgctgaaggg gtatgttttg tccggattgt aaattcatat taactctcct gatttttgat
     4921 tattattaat gcgcagcgtt tatatatgtt ccctaggctt agttctggac gctggatatt
     4981 cggtgaggcg taaatatggt atgacaccat ttttcataac gctgaagttt ctatacctgt
     5041 tgaatttgaa ttttcattga ccgggtatct tattttccag ggcccgttcc ttcataagtc
     5101 gcaaaagtaa catatatccg aagggcatgc tgttgatatc agacacggaa tactggcttt
     5161 aaccagcaac cacagataaa cccggggcct gcataaaagg ttcatgccag aattaacata
     5221 gctctgcttt ctgccccccc ccctttccca tattcacccg ttgatagcgg atcgataccc
     5281 aaaaaaaacc ccggcattgc cggggtcaga ctcagcgtct tttagacatt gacgccgtgc
     5341 tgggaagcaa gcgctgacag accaccggca aaaccctggc caacagcttt aaacttccac
     5401 tcagtaccat ggcgatacag ttcaccgaag accattgcgg tttcggttga ggcgtcttca
     5461 gacagatcga aacgggcaat ttccgtcccg ttgtcgttgt tgtaaacgcg catgaagctg
     5521 ttgctcacca tgccgaagtt ttgtttacgc gcttctgcat catagatggt aacggcaaat
     5581 accagttttt tgatgtctgc tgagactttg gtcagatcga ttttgacctg ctcatcgtcg
     5641 ccgtcgcctt caccggtacg gttgtcgccc tggtgctcta ctgcgccatc agggctggtt
     5701 ttattattga agaaaatgaa atgggcatct gacagtactt taccgtcttc acctactgcg
     5761 aatacggaag cgtccagatc aaaaccctga ccatcggtta cacgggcatc ccagcccagg
     5821 ccaaccatag cgacattcat ggttggtgct tctttggtca gagatacgtt gccgcctttt
     5881 acgagagaaa ctgccatttt tagctcctgc aaacagttga atgaggctga attaacagcc
     5941 ccagatatga aaagttactt ttcgatcagg acgcgttaat gccgtactga gcacatacag
     6001 atgccagacc accagcataa ccctggccta ctgcgcggaa tttccactca ccattgtggc
     6061 gatacagctc gccgaacagc atggcagtct cagtggacgc atcttcggtc agatcgtagc
     6121 gagcgacttc agtctggtta tcgtcattaa ccagacgaat aaacgcaccg gatacctgac
     6181 caaagctctg gcgacgagcc tgagcatcgt ggatggtcac aacgaagatg atcttgtcaa
     6241 cttcagacgg gacggcgtcc agtttaattt tcagcgattc atcatcacca tcgccctcac
     6301 cggtgcggtt atcgccggtg tgcgttacgg aaccgtcgga tgacgtcagg ttgttataga
     6361 agatgaaatc tgaatcgccg cgcactttgc cgtttgaggc cagcaggaat gctgaagcat
     6421 ccaggtcaaa gtcctgaccg tctgttgaac gcgcatccca gccaaggccc accaggacat
     6481 ttttcattga cggagctgct ttactcaggg agacgttccc gcctttggaa agagaaacac
     6541 tcataaaata acctcttcga ttagtaattg ttcaggttaa cacttaaggg gattaactcc
     6601 ccttttcctc agattcaggt ttgcccggga acatgacgct tgcgagaatg cccagcgcca
     6661 gtacacccag tacaacatac aggctggttg ttgccgcgat gctgtaacca tgatgccaga
     6721 tgtgatcggt cgcattcagg ccgagttttg ccgcgatgaa gaacagcagc acgataacgg
     6781 ccttctccag atgaaccagg tactgtttca gtgcctcaag gacaaaatac agagtacgca
     6841 ggcccaggat agcaaacatc atggcactat agacgataag cggttcacga ctgacggcaa
     6901 tgattgccgg taccgagtca aacgcgaaca ttacgtccga gagttcaacc acagccacac
     6961 acaggaacag cggggtcgca tacagcgccg cttttttacc acggccaatg gtgacatcgc
     7021 tgttttctgg tttcgccagt tcagcatcca cttccttctg gttaagtagg aaggcatgcc
     7081 ctctgagctt cggccagata gggaagaagc gtttaaccat acggtaagcc agatgctggg
     7141 agtaatcctc aatttcatca tcgtcatcac cgcttttaag catcatgacc gccgtccagg
     7201 caacgataat agcgaagaca acttcaacgt acggccccag actcagcagg ctcgtaccga
     7261 tggcgacaaa gatgcccctg aagacgatgg caccaatgat cccccagtag agaacacggt
     7321 ggcgataacg atccggaacg gcgaaccagg agaagattgc catcatgacg aacaggttat
     7381 cgaccgacag cactttctcc agcgcataac ccgtgacaaa cagactggca acctcagcac
     7441 cgtggtggat atagaggaaa ccggcaaatg ccatcgcaac cacaacccag aatacggacc
     7501 agagcgcagc actcttcagc gagattggct tgtcatcacg gtgcataaac aggtcaataa
     7561 agatggcacc gactgaaagc gcaataaaaa caatgaccgt ttcagtcggg aagccgatgt
     7621 gtgtggaaac catattttac ccttagggat gtggatcaga ggccaaattc agccgggtcg
     7681 aagcccaacg cgatagcgat ctcgcgggag gccagtttct cgtcctgatc gaagttaccg
     7741 tcacttttcg caacggcaat accaacacgt aaggccagct gagcggcctc aggctgatct
     7801 ttcagcgcca ggatgtattt catggtttcg cccttgccaa tttcaacatc gaaatcgaag
     7861 cttgaaacca gtttattgaa gaactcaatc acctcattgg tatcgaagac cttcagctct
     7921 tctgaagagc gcagaaagcc catcattttc tgcttttctt ccgaacttac gccgtcactc
     7981 gatacggcaa tacgggcaca tacagcaacg gtgccctgca tgaatttttt gtttttgaaa
     8041 cggccaacct ggcgggtcag ttcgtcacgg cctgagttaa tggcaccttt aactttgtcg
     8101 aaaaagctca tacctgtttc ctttgttgct gtgttattta gaaccggcgc tccagcgaaa
     8161 tccccagcca aatgcccggt ccatttcgtc ctggcctttg aagtactggt taattcgctc
     8221 gactttaatt gcgccgtttt cgtttacgag tctggcaatg gcgcacaatg tgcgacggtt
     8281 ctcaccttcg gtcaggcggg tctcaatggg cggttgatcc ggcacatgaa tggtgactac
     8341 accatcagtc ttgtcccagc tgggaacacc ttcataaata aaggcgtaaa tcagcacttc
     8401 gcggatatgc ttccattcac gtccattgat atgcagccac tcgccgtctg atacatcacc
     8461 cgtccggtca tcgcccttga gctgaacata cggttcatcg cgataatcac cgaatgcatt
     8521 accgagcgcc tgaatgaccg atttataccc gtcctgaagc tcaacaaagg cccccagatc
     8581 caggtcgatt cctttggagc cgaacatacc tgcaaagcct gatttgctac tgccccggtg
     8641 ccagttcagg ttgatccgga tttcaccgaa gttatcccgt ttggtcaggc tgattgcagg
     8701 cttctctttt gtcagcgata ccttgctcag gctgattttt gtctcaactg gcggaggggt
     8761 tacaaccgga ggagttgggg ctgcgggagc cggttcgtcg gcaatattta cgccgaaatg
     8821 ctctgccagc ggtttaagtc caccgttgaa cccctgcgca acgaagcgga atttccagtc
     8881 attattacga cggtaaaatt cccccagaat taaggctgct tcctgacggc cgctcagttc
     8941 gacactgccg ctaaccaggc cggtagcccc ttgctccacg tcgatggaaa gattacgaag
     9001 accggacacc gtctgcccac cgtcacaggt cacggtgaac gcaattttct ggacatcagg
     9061 tttcagccgg ttgagcgcga cagtaaaggt tgagtactgg ccttcactga ccaggctgac
     9121 ggtaccatca tcgttacgtg gctgaccata aaacaccatg tccgcatcac cctgcacctt
     9181 cccgtcggca tacaggcgaa acgccgaagc atccacctgg ccgcccgagg tgatccggac
     9241 ccgcaactcc tgggctggaa cgggggcatt gccccccggc gtcaggttca ttagcgaata
     9301 accgctgaaa cgatatcgga gtgcatatcg tcaattgtgc ggccgcgaca ggcatggcca
     9361 agggcggtaa agtcccagtt gccgttatta cggcgcagcg aagaaataac gatcccggta
     9421 tgtgagccct gctcagtcag cttgtagcga gccagctctt tgccggtttg atcgacaacg
     9481 cggcagaaag cgttttcgac gtcattgaac gactgaccac ggaagctgtt taccgtaaac
     9541 gccaggtatt caacgtttgc aggcaggcgg gagaggttga ccttaatcac ttcatcgtca
     9601 ccgtcaccct caccggtcag gttatcgcca ctgtgcacaa cagcgccgca ggtggatttc
     9661 agtttgcgga accagatggt gtcaattgtt ttacctgtgc tgtccattaa tacacagcca
     9721 gcatcgagat cgattgagtc gttgcctcca aacaatcctc caagaagacc ttttttctta
     9781 accggatccc aaccgagacc gaaatgaagc tggcttaatg cagatgattc tttgctgagt
     9841 gataccgtct ggttcttaac taatgaaacc atgttttatt ccttaaaatg gtaatgaccg
     9901 agacaatcaa aggtatattt agtaactgac gaagaaaatc atatcatgat gaagttctct
     9961 gacaagggaa atttttgtgg ttttctgtaa aaacacaaaa agaaatcctt ttcagggtgt
    10021 tagtttttct atttttaatt taaatatcaa tgtgttataa ggaatccaaa aaacactgac
    10081 gcactccata accacaggag ttattaatca tattatgatt gacatgtagc ttagacactc
    10141 atagactcac ttctcaatca taaatttgca tggcgttccg gataattaaa aaaaatgaac
    10201 aagaaaattt ggtttatgga aggtttatcc tcccagcgag atattattca gggggtaaaa
    10261 tcatttgcac aaaaaaataa ttttgccatt accgtttttg cctcccaccg taacgaaaga
    10321 aatgaaatcc tttccgttgc cgattattct ttgactgaac ctgaagatcc tcaaaaacgt
    10381 cttcagttta tccaggaaac cattcagacc tacggcatcc accatattca tactggccgt
    10441 aacagccagt ggtttgaaga acaccgttca gccattgaac cgaccggtgc cacccttact
    10501 accggtgcaa cgggcgtcga ctggttaact ctggctgacg aaaaagttac ttttgctcag
    10561 tttatggagc aaaagggtct cccggtcgta ccatcctggc gggtgaatac gctggcagaa
    10621 ttaaagacac acctcgcggc cccgccgttc actgacagcc cggtatgcgt gaagccggtg
    10681 acgggtatct atggcatggg attctggcgc tttgatgaca gtgcttcgcc tatggccgtc
    10741 tttaatcatc ccgaacatcg tctggtcagt ccgcaacagt atattgcagc agcatcagct
    10801 gctgagtcgt ttaaacccct tgttttgatg ccgtacctgc caggcccgga attttccgtc
    10861 gatatcctcg cggataaggg cgaaatactc gcagccgtgg gacgccgtaa ggaaggggct
    10921 atccagtatc tggtaaacga aggaagcgcc tgggaactgg cgtgtgactg cgcccgtgtt
    10981 atgaaggccg acgggctggt gaatgttcag acgcgaaacg atgtgaatgg caacccggtg
    11041 ctgcttgaaa ccaacatgcg tccgtcaggg ggggtgggtt atacccttca cagcggtgtg
    11101 aaccttcctg ggttatttgc tgcctttaag ctcggtctga tgtctgaaga tatggtacgc
    11161 cagagcgcta aaaacacctt ttctccggtt gcggtgagat ccattacgga tgtaattgca
    11221 tacccggaat cactctctaa ccttctgaat taaggcgtat ttatgtctga tatcctcgaa
    11281 gatatgattt atcgccgtac cctctcatgc ggtacgattc aggtaacccg tgaccagggt
    11341 gaagtttcgc tcgatgacct gtttgacatc gctgaaagac gtaacccgaa acgcgccttt
    11401 ctgtttgtca gcaaagtact cggcaggcac attcccgtgc caccctcagt catgcgacag
    11461 gcctacagac agcttgccag ccagttcccc tcgacactga caggacccgt actgtttatc
    11521 ggtatggctg aaaccgccgt tgggcttggg gccggtgtat ttgatgaagt gcgccaccag
    11581 catcgtgaat ctgtctatct gacctctacc cgtcacccgg ttgatggcac gttgctttgc
    11641 gagttcaagg aagaacacag ccacgccacc gatcatctga tctatctgcc agatgatgaa
    11701 gagaaaagac gtcgtgttac caacgcacga acgctggttt tgattgatga cgaggcaacc
    11761 accggtaata cctttattaa cctgctttct gccctgcgta ataccggcaa gcttcaacat
    11821 attgaacagg ttatcgccgt aacgctgacc gactggagtg gcaatgccct gtcggagcgc
    11881 acccccttgc ccgtcacttc ggtttcgctt gtaagcggtc agtggggatg gaccccatta
    11941 cctgatgcac ctgtcccgga catgccgaaa gtcaacgtaa cttcacgagg agaatgggac
    12001 atccagggga aacagtcatg gggccgactg gggatgctcg cacctgcggc cgatctcggc
    12061 catgaggtct ccgtccacaa gggggaacgt attctggttc tcgggaccgg ggaattcgtc
    12121 tgggagccgt tcctgcttgc tgaacggctc gaagctgccg gagcacaggc attttatgga
    12181 tcgaccaccc gctcccctgt cgccgttggt tatgccattg agtccgccat ttcctttacg
    12241 gataactacg ggctgggcat ccccaatttt gtctataacg tcgcccacca gcagtttgac
    12301 cgcattcttg tgtgtactga gacacccgca gaaagtattg acacgcagct tcttaaagcg
    12361 ctggctgagg ttgcgcccgt cgtggagatt gttacctatg aataaacccg ttgttctgag
    12421 cgaccttgac gacacgctct ttcagacccg tcgtaaaatg gtggacgagc tggccctgga
    12481 gccattccgt accggtgccg ttgatcgcac cctgaatcca cgaagcttta tgacggaaga
    12541 acagtccatg ttggtggact ggctcctgga gcaggctgaa ctcatacccg ttaccgcacg
    12601 gggaactgaa gaaatcagcc gcgtacggat ccctttccac tcctgggcaa tcacaacgca
    12661 cggggccgtc attctcacgc ctgaaggcaa acccgacgag gagtggaagg cccatatgct
    12721 cggccagctg gctccctacc aggaaaagct gacatcgatg cagcgtctga tcactgaaat
    12781 gatggacgca aaaggaatca atgcctgggc aaggctgaac ttcgaatacg gtgaaacggc
    12841 ggtttatatg gtgatgaaac accgcgacag cacccgcctt gacgagctca atgccattgc
    12901 agatgagata gaaacggtgt ttccgaccga gggcttctac atccaccgca acagtaataa
    12961 cgtggcctgg cttcccaccc cggttgagaa agggctggca gtcagctggc tgcttgaaaa
    13021 acttcgggct gaacgaggag ttttccccgt aattggtttg ggcgacagcc tgagcgatca
    13081 tcgttttatg aaactgtgca gctggtttgg catcccgcgt cagagtcagt ttgcagatgc
    13141 catttcacag cgaatttttg gagaaaatta aaatgcagcc acatgaaaca tttaccggct
    13201 cataccagcc cggtgacgtg gaatttctgc taaagccggt tgtcattgag atgacgccgg
    13261 ttgagaaaaa agaagaactg attcagtccg ggaagaaaca ttattcggac atgctcagcc
    13321 aggagccagc gcctacacaa tggcatcttg atttgtttca ccgggcgctg gatcggggcg
    13381 cagagaggct tgcaaaagaa gtcacacagc tcgctattgc tctggccgaa cgcttcggtg
    13441 atgagcccat tgtactggcc agtcttgtca gggctggcgt tccgctcggc gttatgctgc
    13501 accaggccct gcgtgacatg gggaaaacct catggcatta cggtatcagc attatccggg
    13561 atcgtggaat tgacggtgca gccctcgacg tcattgaaga gcggcatggt accagcggta
    13621 ttgtctttgt tgacggatgg acaggtaaag gcgcaattac cggagagctt gtacgcgcac
    13681 tgaaagatcg cccgggctat cctgagcagc cgcgactggt tgtcctggcc gatccctgtg
    13741 gctgctcctg gcttgcagcc agtgatgatg actggctcat tccttttggc atcatggggg
    13801 cgccggtatc aggcctgatc tcccggtcag tatggtcctc tgaaggatta cacgggtgca
    13861 tggtttgcga gcatctcagt gaattcgaat gcagccggat gcttgccgat accgtcgctc
    13921 atttccgtaa gaagttaaca ccgtcatccc tcgctcccct gagctggaat acggaatcag
    13981 cccgggtctt atggcagaca agtcgcgacg ttatcgcgtt cctggccgat gaatttaaag
    14041 tggacagcgt caatcgtatt aaacccggta ttgctgaagc aacccgggct gtattacgtc
    14101 gggtaccgga ccatgtattt gtgcgttcta ttgacgaccc ggacgttgcc ttgcttgtag
    14161 ggcttgctcg tgaaaaggga atagttgtta cagaaatggg gggaaccctc ggccagtatc
    14221 gggctgtaac cattatcaag aaggtactct gatgaaaaat cgtctttctc cctggaatct
    14281 gggagccacg ctatacatgc ctgcaacacg ggaagatatt gctgatgccg tcctgcacgg
    14341 gaaaatcccg ggcttgcgct ctctggtgat ttgcctggag gatgctgtca gtgaagcaga
    14401 catccccgtc gccctcaaaa atctggagca cctgctgcac gagctcagta acagcatgag
    14461 tagcctgggt aaaaatgact ggcctctggt gttcattcgc ccccggcatg ccgaaatggg
    14521 cagatggtta aaagcgcatt atgatctttc cgctgtcgac gggtttgtgc tgcctaaatt
    14581 taccctcagt tcgttagctg aatggtggga tatcatggtc gggactcacc tgtgcatgat
    14641 gccaacgctg gaaacagaag atgtctttga cgtggttcag atgcgcgagc tggctacccg
    14701 tctggaggaa catccctgcc acgaccgcat tattgcgctt cgaatcggcg gcaacgatct
    14761 tatgaacgtt gtgtcgcttc gccgtccccg ggacctgacg ctgtatgaca gcccgatggg
    14821 ctacgtcatc aaaatgctcg tttcggtctt cggcccgcgt gattttgccc tgaccgcgcc
    14881 tgtatgtgag catattgacg atcatgccgt tatggccaga gagctggctc ttgatatggc
    14941 acacgggctt gttgggaaaa cggccattca cccgggtcag atagaggtca ttcaaaacgc
    15001 gctgatggtc actcagggtg agcattctga cgctctgcgg atcctgaact ctacccaggc
    15061 cgtgtttaag tcgcagggag caatgtgtga acccgccaca catcgccgct gggcggctgg
    15121 cattctggac agggctcgtt tttatgggtt acagaacgag caaagcgctg atggaatcag
    15181 attacttacc gtgacccagc atcattaagg aaatcataat ggggttcagg ttcagaaagt
    15241 cgatcaacat tattcctggc gttcgcctca acctgagtaa cggtgcaccg agcctgagtg
    15301 tcgggccgag aggtgcttcc gtttcttttg gtagccgggg gacctatgcc aatctgggct
    15361 tgcccggtac cgggctgagt taccgtaccc ggcttgaccg ggccgcgcgt tccagaggtg
    15421 aaaaccggac ggcaaccgac ccggggctca gacaggcgct tgagcagaaa gccgctgaac
    15481 tcatgtcagc ggtaaccgca atccgtaata ttcacgagct gacgccggat ccaaaaacag
    15541 gcatcagctg ggcagagctg gaagcagtat acctgcataa cagaacgtcg ccttttcagg
    15601 ttccggcacc ggtgcgtcca gaaaagccag actaccttgt attgccggaa aagcctgccg
    15661 agagcgaagg cattagtttt ttgggtaaat ggtttgaatc ggaatcagct aaagctgagc
    15721 gccacgccga aaatcttcgc cggtggcagc aggagctgat tgatgtggag cgtgagaata
    15781 cccttcgaca gcaccggtac cagcaacaac ggacggcctg ggccgaacag tatgcaaact
    15841 ggaagtttga agctgaagaa catgaaaaac ggctcgccac ggctcaggca gatgcccggc
    15901 agcagttccg gacagacgcc gcgtttttcg aatcatacct ggcgggtgtg ctggcagaaa
    15961 ctgaatggcc gcgtgaaacg cttgttgcat ttgaagtaaa gccggagcta tcagcagtcc
    16021 tgctggacgt tgatttagct gagattgaag atttccctga taagatttac ggcgttaatg
    16081 cccggggaac ggagctgacg gaaaaagcca tgacgcaaaa agccgtacgc gaaaactatg
    16141 cccaccacgt ccatggctgc ttgttccgcc tggtcggtat cgttttacat acgctacctt
    16201 tcgacaacgt gattgtgtca ggctttacgc aacgggtcag taagcggacc ggctatctgg
    16261 aggatgagta tatcctgtcc tgcaaatgca ctcgcagcca gatgtcgtca gtaaattttg
    16321 caggcataaa acacattgat ccggttgaag cgttaggcga tcaaccggtt attcgaaaga
    16381 tgagcagtac cttcattttt cagcctattg aaccactaac cctttaagca ggctaaatct
    16441 tacgatgcaa ttaaatacca gacaggcccg gatttttaag cttgccaact tactgggcac
    16501 cggcaagccc gtttccgctg ccgacattat aaccagtctg gaatgctctg agccgacatt
    16561 aacccgggcc ctcaaagagc tacgcgagtc atattctgcg gagatcaaat acagcaaggc
    16621 aggtcattcc tatcatctcg ttaatccggg ccagttggat aaaaagaccc tccgccggat
    16681 gaatgaagcg ctcgcacaga atgctgaact gaaaaccggt gagtccacag ggaaggtcgt
    16741 actcgataag gataaaaaaa cagccgtgtc attatcgcta cgcatgcgga ttctaagaaa
    16801 aattgaccgt ctggctgcgc tgagtgactc gacccgaagt gaagcggtag agaagctggc
    16861 tctgcactcc gttgatgaac tgataaaaga atacagcgct aaaaagtcct gacgctaaat
    16921 ctcagaccgg ggtgaaaact ccggttttgt catatttcac ctctgtattg gcgtgatgca
    16981 aaatggcatc tgaatcgatc atggtttggc atccatttcc gatcacagtt tggcatcaca
    17041 atcgatcacg atttggcatg cttccgctca ttgattggca tcctggtgtc gttacgggga
    17101 agtcatcttc acagggatct tcagtcagtt agcattaccg acatccattc accaacttac
    17161 cggagataat caatgtcaaa accccgatgg acgcttgatc agaaaaaaca tcacgttgcc
    17221 gcatggcgcg ccagcggcct tacccgtgaa cagtactgtg aactttacga cattccgttt
    17281 aaatccctgc gccagtggcc gcaggatgtc gcaaaagccg aaaaacgagc cagagcacca
    17341 gaaattattc ctgtttcagt atctggctct tccggaatga ctgatggcag accattgtcg
    17401 gatgagcctg tcactctctt cctgcccggt ggtatccgga tgtgctgcca gccttctcag
    17461 ttaaccgatg ttttccgggc tcttcgccat gctgacgcct gataacgtat tcctggtcgt
    17521 aaaacccgtc gatatgcgcc ggggtatcga cacactgaca cagtacgtcc agaacgaact
    17581 taacgctgca tggcatgacg gtgctgcttt tgtcttcacc aataaagtcc gctcccgcat
    17641 aaaagttctc cggtgggaca aacatggcgt atggctgtgt acccgacgac tgcaccgggg
    17701 aagcttccgc tggccccgga aaggtgatgc cacatggcat ctcacacagg atgagtttca
    17761 ctggctggtc tttggggttg actggcaaca ggtgaaaggg catgacctgg caaaatgggt
    17821 ataccaggaa gaacccgaag atgctcagag caacgcaata actcaatgaa atataatgaa
    17881 tatccatgcg tgatgtcata taatccaccg catggatatc tcgcttctca gtaccaccag
    17941 tgaccctgaa cagcttcgcg ctctggcaat tgccatggtg caaaaagtca tggctgagaa
    18001 tgcagaactt cagaaccgta tccgcatcct ggaagagcag atgaagctcg cacgccagca
    18061 gcgtttcggt aaaaaatgcg aatcgctggc cgggatgcag cgttccctgt tcgaagagga
    18121 tgttgacgcg gatatcgcag aaatctcggc gcatctggac aaactgcttc ctcagaccgg
    18181 ggacgaagaa aaaacaacaa cacgtcctgt tcgtaagccc ttgccttcac ctcttccccg
    18241 tgctgaaaag gttatcccgc cagctgagga acgttgtcct gactgtgatg cccccctgca
    18301 cttcattcgc gatgaagtca gcgagaagct ggaatatatt ccggcacagg tcgtggtcaa
    18361 ccgctatatc cgcccgcagt acagctgtcc ctgctgtgag aaagtgttca gcggaaaaat
    18421 gccggcacat atccttccga aaagtgctgt tgagccatcg gtcatcgcac aggttgtcat
    18481 cagcaaatat accgaccatc tgcccctgta tcgccagcag cacattttct cccgaatggg
    18541 ggtggagctc cccgtcagta caatggcgga tatggtcggt gttgctggcg ctgcactggc
    18601 cccgctggca aaattgttac gccacgagct tctgacacgg gatgttattc atgcagatga
    18661 aacgtccctg aggctccttg atacccggaa aggcggtaaa tcctgctccg gctggctgtg
    18721 ggcttacgtc agcggagaaa gagtaagcgt caacggagca ccgtattgac gcttatttat
    18781 tggtgagtac tacgttccat ggcaggagtt catcaactcg gttggaaggc cattctggca
    18841 gtacgctcag gatatggcgc agatacgctt ccggatcgat accgttcaga cggcaggtgc
    18901 cgatcagccc gtacaacagt gctccacgct cgccgccgtg atcgctgcca aagaacataa
    18961 agtttttctt tccgagacag actgcacgaa gcgctctttc cgcagcatta ttatccgcct
    19021 ccgccagacc gtcatcactg taataacaga gggcatccca ctgattcagt acatagctgc
    19081 acgcttcgcc cagtctggat tttttcgaca gcgtaccatt cttctccacc atccattcat
    19141 gcagtgacgt cagtaacact ttgcttcgct gctgcctgac ggcaagacgc tctgactccg
    19201 gtaatccccg tatttcatcc tcgatggcgt acagttcact gattcgcttc agggcttctt
    19261 ctgccgtcgc acttttgctg ctgatgtata catcgtggat ttttcgccgg gcatgggccc
    19321 agcacgcaac ttctgtcagt gcaccacctt cacgttctgc actgaacagc cgatcgtaac
    19381 cgctgaacgc atccgcctgc aggatacccc ggaagggacg aaggtgttgt accggatgtt
    19441 ttccctgcct gtctggtgag taggcgaacc agaccgccgg tggctctgat gagcccgcat
    19501 tccggtcatc ccggacatac gtccagatgc gtcctgtttt tgcctttttt ctgcccggtg
    19561 ccagcacttt tactggtgtg tcgtcagtgt gaaccttgcg ggtgttcatc acgtaacggt
    19621 acagggtatc attcagcaga gtcattaact gacaacatgc gtcaacccag ttggagagta
    19681 atgcccggct cagttcgatg ccctggcggg caaagatttc actctgacga tacagtggca
    19741 ggtgttcgca gtattttccc gttaacacgc gggcaagtaa tccggagccc gcgatgccgc
    19801 gctctatcgg gcgggacggc gctggcgctt caactataca gtcacatttt gtacaggctt
    19861 tttttacccg aacagtgcgg atcactttca gggcgctact caccagttcc agctgctcag
    19921 cactgacttc ccccagataa tccagctcac tgccacactc cgggcaacaa ctttcttcag
    19981 gctccagacg gtgtatttca cggggaagat gtgccggtaa cggacgacga tgacgtgact
    20041 gtcgcaactg gcggggaacc tgcgggtcat cctcacgccc actgtaacga tcgctttcct
    20101 gttcgcgttg tttcagttgg gcctcagcct gttcaacctc acgctgcagt ttttcagaac
    20161 gggtaccgaa caacatccgg cgcagttttt ctatctgggc cctcagatgt tctatttccc
    20221 gctcctcctc ttcgatcttt tcctcggcac gtgccagtgc agagcgcagg aaggcctccg
    20281 tctcttcaac cagactcagt tgctgatctt tctgacggag ggcttcagcc tgctcagaga
    20341 gcagcctttc cagctcagtg atacgaatga ggtatttccg actcatgacc gtttttataa
    20401 tccggccatg acatttttac aacattgtca gtgcattaag gcgggatgtt ttgggctgac
    20461 gccagtccag tttatcgagg agcattgcca gctgcgagcg ggtaatggat accttaccgt
    20521 cacgcaccgc aggccagata aactggcctt cctccagacg tttggtgaac aggcacagac
    20581 catcagcatc agcccacaga attttaatcg tgtcaccccg tcggccgcga aagataaaca
    20641 ggtgaccgga gaaggggttc tcatccagca catgttgtac ctgttctccc agaccgttga
    20701 aggatttacg catatcagta acgccggcaa ccagccagat tcgagtgcct gatgggagcg
    20761 agatcatcgt cccctcccgg tcagttcacg gatcaacacc gtgagcagct ctggtgaagg
    20821 attttccagc gtcatgttac cgtggcggaa ctcaacttta caggaactgg cactgactgt
    20881 gctttgtgaa ggagtggata aaagcggagt aagagccgcc ataggctctt tctgctcatc
    20941 aggcgttatc tcaacaggta ataattcaac gccagcgcca gaagaggttg ttaccggaag
    21001 acgccgcgat atacgccttt cgttctgcca gagcctgagc catttgaaca ggaggttatc
    21061 attgatatcg tgttcccggg caatacgggc aacagaggct cctggttgtg aagccagttt
    21121 aaccatttga agtttaaact catttgaaaa tgatctgcgg ggttctgagg ataatatttt
    21181 ctgttccata acaggtgtcc actagttgaa aaggtgggca cctacgttac caatactggc
    21241 ttaatggcta catacggcgg tcagtttacg cttacgagaa agaagcggtc cgccggtcgt
    21301 ctgttttgac tcgcagaccg gacgggccct cagatatcct gaggcctggc tgcagggctg
    21361 gtgtggcggt acgctggtga gtgacggata cagcgtgtac aaatctctgg cggataatga
    21421 ccccggtatt acatctgcct gttgctggag ccacgcaagg cgcggtttcg caaatctgta
    21481 taaggccagc agggagcccc gtgcagccat ggcgctgaga aaaatcgcgg gtctttatcg
    21541 cattgagaag ttcatccgtg aacgtcctgt ggaaaaaata cggcaatggc gacaacggta
    21601 ttcccggccg atagtgaatg acctgtttgc ctggcctgaa gagcaggagc cgtgctgtcc
    21661 gcccgacgga ccactaaaca aagcgattaa ttatatcctg aaccggcgcg atgaactgag
    21721 ctgcttcctg agtgacggag ccgtgcccct ggacaacaat atctgcgagc gtgctatccg
    21781 cccggtggtc atggggcgga aagcatggtt attcgccggt tcactgatgg ccgggaaccg
    21841 cgcggcacag ataatgagcc ttctggaaac ggcaaaacgt aatggtctgg agccgcatgc
    21901 ctggctgaca gacgttctga cgcgtctacc ggagtggccg gaagaccgac tggaagagtt
    21961 gctgcccctt gagggattta ccttctccgg gtgagtgata cctgccatca ggtgttcgtg
    22021 cagcgggcca taacccgcag tcgggagttg aacgcctgac gacaggaaat gagccagagc
    22081 agcgaccacg cgggctgaca gtgagtcaga acctgaatca gcccggagcc attcccgggg
    22141 ctgtacttca tcacctgcca tcagtacccg tgtcagtact tcgcgacctg acgcaattac
    22201 ccgactcagt tcttcagaac ccgcctccga taccagcctg gcggtcgccc cggcgcaaca
    22261 gaataaatca accatcgtca gactgcgatc attcgtatgc atcgctgatg atgcgcccga
    22321 aaaacgcgtc aatatgagtt cgccggaata ttactgacga cacggtttgt agttacgttc
    22381 cttcggtgag tgagtacctg tgcgtcaggg taacgcaatc agttcaggtt tagcggggtg
    22441 tcaagtcggt ttggctaacc gaccgcagaa ttaactgtac ctttaaaggt cgcccccgat
    22501 atatctgcct gtcagacata tcgccgattc ggtatctcac tacataaaac tccttaaaaa
    22561 acaatgcgat tctgttggta cgaagctttc agtaagtcag tccggagaag cgccagtacg
    22621 atacagaatg aggttgaaaa aaacgttcac gtctggtcac ttcttcaaat gttaaaaaac
    22681 atctcgtaaa gagccagttt atgcgggttc gttgagtaga cgtgcgagcg tcactttgtc
    22741 agctttaacg ctgacgaaag tgacgcccgc acagcttcaa actccacatg cacacgcaag
    22801 cgaccaaatt tcaaatggtc ttatgccgtg cgcacagtca taagaagcgc atgcggaaag
    22861 tcagaaaata tcttaagacg cagattttaa aggcgttttt atgccagtct gccgggaata
    22921 cacagtagtc cggttgtccg gaagcaccac ggtaatccga aggaaaaatc aacgtacctc
    22981 aatcaccgta gtgatatccg ttcccagagg ctaaacggat gtaccaccat aaaggcatcc
    23041 tctttcaggc tacgaggata ttgcaatcac ccgaaggcgt ttctctcaga ttgctgaaac
    23101 attggctggc agtcacccga aggcgtccct ctcagactgc tggaacattg actggtcgca
    23161 aaagcgacag ttaatgcaaa tacaggttaa caaaaaggga aatctgccgt caacccaacc
    23221 atatgaacat gttgatttca ttatgaatgt gatgttctaa tataaattaa tcagtaactt
    23281 cataaaaaga atataagaaa cagataatgc atgtatttac acacatatca gcacaatgat
    23341 agattaccac cccagaaaca cgataaaggt ataattatgg aattttccgt tatttttttc
    23401 tgggttatca ctggtcttat taccatctta ttcttaaaat caataataac cgatctaata
    23461 aaaaacctaa acaaaacaga catagcgtta tatatattgg catttttatc agccggatat
    23521 acattccagt atatttacaa tgacaatata ttttcagaac tatggggagt tggcctgttt
    23581 gttactctga tagtactgaa tatagtattt aatctctttt ttagactctt attcattctc
    23641 tcgaaaatga tattaatgtc acgacatata agaaagatgc aaaaaaaact aaaacagacg
    23701 tctgtgcctg attttattta ttattcaata gaaaaacaca acaaatatcc catatatgta
    23761 ttacacatgc catcaaacaa tataattgaa ataggctaca atataataaa caccgatctg
    23821 gtcattggcg aaaaaattca tttcaggaca ctaagcaatc gttctttata ttttaacctc
    23881 acccaacccc cattaatcgc cacaataaaa gatgatgtgt tttgcactct tcatgattac
    23941 tataaccaca ataatgaaac gaaatccact attgacaatt acatatcaaa aataaaggat
    24001 aatcataata ccccatggtt attaaacaac gaaaatgttc aggaataaaa aaaagagaca
    24061 attcccgaga taaaacaaaa gagcataaac atatgaaccg gaaattctat ttcaaaaata
    24121 tcctgtgggg aatgtgcccc attataatat ccatattgca tctgatatat gacacaccaa
    24181 cagaagcatc gataaaagtt gacttatttc tgatactcag tggcctggcc cttccttgtt
    24241 caatatacct gacaaaaaca atcacactaa aatacacatc catatcattc tggaaaaaac
    24301 ctcttttctg gtcttatggt atatatcatt ttcatcttgt ttatataatg acatgtatag
    24361 ttttagcaat accttctgca attatcgttt tattctttca cttttatcaa aacatcacca
    24421 gaaactggaa gtgacaaccg caataatgaa cataaaaagg caaatcagaa tggcagtaaa
    24481 tcaataaagt accatttcat atgaatcatc agaaaaacgg tggacgcgac atccagcacg
    24541 aggcaaaaca cagcaacaaa ataagccgga tatcgcgccc tgtacttttc taactcaact
    24601 cccggcattg tgcaagtttt aatcgcaggt ggattcagag ccacgccccc attgattcag
    24661 cctgaagaca cgtatatcct tggagaaagc aggaatctca ttgctgtgaa attccttaca
    24721 gaatcttcag ccccatttta tggggctttt tttggtgccc ccacgctgtt accacacttt
    24781 cgggatgcga ataaaaaagc ccacagtttt tacactgcgg acttttgttt tttctttgtg
    24841 atttgtgtgc aagggtgcga ctttcgaccg aacctttggc aaccggatag cggggactgt
    24901 tgtccccgct gcggtttctt acggtttaca ctgcaagaac gccgcaattt ccgaccacat
    24961 gaagcttacc ctgaattcac acggtgatgc gtgtaacacc cagacggtca gcaacatggt
    25021 caggcataac atgcccacaa tgatcgtttt ttgcggcata gcacttgctc cttttacaga
    25081 gaggcgctaa ccttccactt gctgaaggta gatggtcagg gcctcgggtt aacgtttaag
    25141 ttgactcggg gcctttccac atccggcctt caggtaatcc ttccagccgt cagccgaaag
    25201 gcacccgcgc gtaatgtact ggttttcaga gaggtccgca atcttccggt gacgagatca
    25261 tctgcacacg ccccggtggg acgggctaac ggtatattca ggtgcaggat ctctctgtct
    25321 ggtgcgtttt taaattgtca aacgcaccct ggcggcaaaa tcagccacct tttttcgcat
    25381 caacaaaacg cccaaaacgc tgggtaaagc gtgcaacatt accttctgaa gcgacagttc
    25441 tcagtttacc tgtataaaac ggatgcgact tagaagaaac gtcaattgta acatatgggt
    25501 aagttacacc atccagttca attacccggt cagttttgat cgtcgaaccg atcctgaagt
    25561 actcatcaat actggtatcg tgaaatacta ctgtacggta ttccggatgg atattcggtt
    25621 tcacaacagt tttccccctt taagtgaaaa ttccgcataa aggtacatat tcagacggtc
    25681 agattcaact gtctgttctt ataacctcac tctctttcag tattacaata tgttatataa
    25741 ttataaactg gacttcccga aaaaatccat taaataagaa gcgagccgtt gcggaggctt
    25801 gctctctcct ttaacctcat gtgcaatatc cgtaacgcgc tcacataaat catcatctga
    25861 taatataaca acacccatta ttttattctg acctaaaaac ttcttatgaa ataaacgttt
    25921 acccgacgta tctgatcccg ctgtttttac ctgtatcctt taacatactt gagaagcccg
    25981 ctgaaaaata taccgtaaag gcggttctct gtcttccata aacctgtcgg ataagaaggg
    26041 cattaaatga aaaccaaaaa agttatgatg attgcactgg tcagctctgc gctggctctt
    26101 tcaggatgtg gagccatggg cacagcgatc aaaaaacgaa atctggaagt aaaaacccag
    26161 atgagtgaa
//
LOCUS       DAZFJF010000070        24441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFJF010000070 DAZFJF010000000
VERSION     DAZFJF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.070"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..363
                     /locus_tag="ACHKAU_003017"
     CDS             <1..363
                     /locus_tag="ACHKAU_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO5532034.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHKAU_003018"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHKAU_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO5532035.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="ACHKAU_003019"
     CDS             2185..3504
                     /locus_tag="ACHKAU_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO5532036.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3881..5263
                     /gene="dosC"
                     /locus_tag="ACHKAU_003020"
     CDS             3881..5263
                     /gene="dosC"
                     /locus_tag="ACHKAU_003020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO5532037.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5288..7687
                     /gene="dosP"
                     /locus_tag="ACHKAU_003021"
                     /gene_synonym="pdeO"
     CDS             5288..7687
                     /gene="dosP"
                     /locus_tag="ACHKAU_003021"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO5532038.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHKAU_003022"
     CDS             7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHKAU_003022"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO5532039.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8540..10090
                     /locus_tag="ACHKAU_003023"
     CDS             8540..10090
                     /locus_tag="ACHKAU_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5532040.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10092..11114
                     /locus_tag="ACHKAU_003024"
     CDS             10092..11114
                     /locus_tag="ACHKAU_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5532041.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHKAU_003025"
     CDS             11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHKAU_003025"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO5532042.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12004..12990
                     /locus_tag="ACHKAU_003026"
     CDS             12004..12990
                     /locus_tag="ACHKAU_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5532043.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12983..13909
                     /locus_tag="ACHKAU_003027"
     CDS             12983..13909
                     /locus_tag="ACHKAU_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO5532044.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHKAU_003028"
     CDS             complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHKAU_003028"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO5532045.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14742..14957
                     /gene="bdm"
                     /locus_tag="ACHKAU_003029"
     CDS             14742..14957
                     /gene="bdm"
                     /locus_tag="ACHKAU_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO5532046.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15059..15196
                     /gene="sra"
                     /locus_tag="ACHKAU_003030"
     CDS             15059..15196
                     /gene="sra"
                     /locus_tag="ACHKAU_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO5532047.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15353..17050
                     /gene="maeA"
                     /locus_tag="ACHKAU_003031"
     CDS             15353..17050
                     /gene="maeA"
                     /locus_tag="ACHKAU_003031"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5532048.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17184..18194
                     /gene="adhP"
                     /locus_tag="ACHKAU_003032"
     CDS             17184..18194
                     /gene="adhP"
                     /locus_tag="ACHKAU_003032"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO5532049.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18341..18625
                     /locus_tag="ACHKAU_003033"
     CDS             18341..18625
                     /locus_tag="ACHKAU_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5532050.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHKAU_003034"
     CDS             complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHKAU_003034"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO5532051.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHKAU_003035"
     CDS             complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHKAU_003035"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO5532052.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHKAU_003036"
     CDS             complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHKAU_003036"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22679..22681),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5532053.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23498..24379
                     /gene="yddG"
                     /locus_tag="ACHKAU_003037"
     CDS             23498..24379
                     /gene="yddG"
                     /locus_tag="ACHKAU_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO5532054.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5977 a         6201 c         6189 g         6074 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctaaat gaagacccca
      901 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgctct ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgcct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgcagaa gtggtttccc gctatcccgt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac ggctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtacagg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccacaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagcgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgtttcgttt
     3841 tgattccgtc tgatttccca gccttataag ggtgtgaatt atggagatgt attttaaaag
     3901 aatgaaagat gagtggaccg gacttgtcga acaggcagat cctctcattc gtgctaaagc
     3961 cgcagaaatt gccgttgcgc atgctcatta tctgagtatc gagttttatc gaattgtccg
     4021 catcgacccg catgccgaag aattcttgag taatgaacaa gttgagcggc agttgaaaag
     4081 tgcgatggaa cgctggatta ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct
     4141 aatacaaatc cagcataccg tcgcggaagt gcatgcccgc ataggaattc cggtagaaat
     4201 tgtcgagatg gggtttcggg tgctgaaaaa aatcctctat ccggtaatct tctcttcgga
     4261 ttattctgcc gcagaaaaac ttcaggtcta ccatttctcg attaacagta ttgatatcgc
     4321 tatggaagtg atgacccgcg cgtttacctt tagtgacagt agtgcctcaa aggaagatga
     4381 aaactatcgt atcttctcgt tactagaaaa cgccgaagaa gaaaaagaac ggcaaatagc
     4441 ctcaatactt tcatgggaaa tagatattat ctataaaatc ctgctggatt ctgatttagg
     4501 cagtagtttg cctttaagcc aggctgattt tggcctgtgg tttaaccata aaggtcgaca
     4561 ttattttagt ggcattgccg aagtaggcca tatctcccgt ctgattcagg atttcgacgg
     4621 tatattcaat caaaccatgc gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa
     4681 atttttatta cagataagaa atactgtatc gcaaattatt accttgctgc gtgaattgtt
     4741 tgaagaagta tcgcgccacg aagtcggtat ggatgtactg acgaaattac ttaaccgccg
     4801 tttcctgccg actatcttca aacgcgaaat tgcccatgcc aaccggaccg gtacaccact
     4861 gtcagtgctg attattgacg ttgataaatt caaagagatc aacgatacgt ggggccataa
     4921 cactggcgat gaaattctgc gtaaagtctc tcaggccttt tatgacaacg tccgcagtag
     4981 tgattatgtt tttcgctacg ggggcgatga atttatcatt gttttgactg aagcttctga
     5041 aaacgaaacg ttacgtaccg cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa
     5101 agccgcaaac ggcgaggata ttgccctctc actttccatc ggtgccgcca tgtttaatgg
     5161 tcatcctgac tatgagcgcc tcattcaaat agccgatgaa gctctgtata tcgccaaaag
     5221 acgaggtaga aaccgtgttg aactctggaa agccagtctt tagatgcgcc aggatgcaga
     5281 ggtaatcatg aagctaaccg atgcggataa tgccgccgat ggcatttttt tccccgccct
     5341 tgagcaaaat atgatgggtg cggtgttaat taacgaaaat gatgaagtga tgtttttcaa
     5401 ccccgccgca gagaagctct gggggtacaa acgtgaagaa gtcattggca ataacattga
     5461 tatgctgatt ccgcgggatt tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga
     5521 aggcggtaaa gcgcgtgtcg aggggatgag tcgggagctg cagctggaga aaaaagacgg
     5581 cagtaaaatc tggacccgtt ttgcgctatc gaaagtgagc gccgagggga aagtttatta
     5641 cctggcgctg gtacgggatg ccagcgtaga aatggcgcaa aaagaacaga ctcgacaatt
     5701 gattattgcc gttgaccatc tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat
     5761 tgtgcagtgc aatcgcgcat ttaccgaaat gtttggttac tgcattagcg aagccagcgg
     5821 tatgcagccc gatacactcc tgaacattcc tgaattccct gccgataacc gcattcgttt
     5881 acaacagttg ctatggaaaa ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac
     5941 cggtgaaaaa atctggatta aagcctctat cagcccggtt tatgacgtgc tcgcgcatct
     6001 gcagaacctg gtaatgactt tctcggatat caccgaagaa cggcaaatcc gccagcttga
     6061 aggcaatatt ctcgccgcca tgtgcagcag cccgccattt catgaaatgg gggaaatcat
     6121 ttgtcgtaac atcgaatctg ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa
     6181 cgggatgccg atacactggg cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag
     6241 ctggtcagcg accattcgtc agcgtgatgg cgcgcctgcg gggatcctgc aaattaaaac
     6301 ctcgtcagga gcagaaacca gcgcctttat cgaacgcgtg gcagatatca gtcagcatat
     6361 ggccgcgctg gcgctggaac aggaaaaaag ccgccagcat attgaacaac tcatccaatt
     6421 tgatccgatg accggtctgc caaatcgcaa taacctgcac aattacctcg atgacctggt
     6481 cgacaaagcc gtctctccgg tggtgtatct catcggtgtt gaccatattc aggatgtgat
     6541 tgatagcctt ggctatgcgt gggccgatca ggcattgctg gaagtggtca atcgctttcg
     6601 tgaaaaactc aaaccggatc agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt
     6661 gagcctggaa aacgacgtca gtaacattac ccagatcgcc gatgagctac ggaatgtggt
     6721 cagcaagccg ataatgattg acgataaacc attcccgctt accttgagta ttggcatcag
     6781 ctacgacgtg ggtaaaaacc gcgattacct actctccact gctcacaatg caatggatta
     6841 tattcgcaag aatggcggta acggctggca gttcttcagc ccggcgatga acgaaatggt
     6901 aaaagagcgt ttggttttag gcgcagcgct gaaagaagcg attagcaata accaactgaa
     6961 actggtttac cagccgcaaa tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct
     7021 tgctcgctgg cacgatcccc agcatggtca tgtaccccct tcacggttta ttcctctcgc
     7081 agaagagatt ggtgaaatcg aaaatattgg gcgctgggtc atcgcggaag cttgccgtca
     7141 gttagcagaa tggcgtagcc agaatattca tatcccggcg ttatccgtga acttgtcggc
     7201 gctgcacttt cgcagtaatc agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg
     7261 tattgacggc caccagctga cggtagaaat cacggaaagc atgatgatgg aacacgatac
     7321 cgaaatcttt aagcgcattc agatcctgcg tgatatgggc gtgggcttat cggtagatga
     7381 ttttggtacg ggcttttccg gattatcccg cttagtcagt cttccggtaa cggaaatcaa
     7441 aattgacaaa agttttgtcg atcgttgtct gaccgaaaaa cgcatccttg ccttacttga
     7501 agccattacc agcattgggc aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac
     7561 caaagagcaa tttgagatgc tacgcaagat ccactgtcgc gttattcagg gatatttctt
     7621 ttcccgcccc ctacccgccg aagaaattcc aggctggatg agcagcgtgt taccgctgaa
     7681 aatctgacaa attcctctcg cccgcactcg cgggttttca tttaacgtga cactgtcact
     7741 taaatactgt gatttcagct acgattccgg gagattcctt ccttaacttc ccctcttgca
     7801 ccaaaactgc actacgctgc acaaatgttg atcaaaagtt aaaaaatata actttaagcc
     7861 agcattctgg cgcttatccc ggcatggcat gagatctgca taagcggaaa gcgcagcaat
     7921 ttttgtctta tacaggaacc gtttatgtcg gataccaccg aactggttga tttagccgta
     7981 atcttccctg atctggagat cgaattgaaa tacgcctgcg ctgataacat cacaggtaaa
     8041 gctatttatc agcaagcgcg ttgtctgcta cacaaggatg cgattaccgc gctggcgaaa
     8101 agtatcagta tcgcccagct gtcaggctta caactggtga tttacgatgc gtatcgccca
     8161 caacaagcac aggcgatgtt gtggcaagcc tgcccagacc cgcaatatgt tgttgatgtg
     8221 acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc tgacgcttcg tgatgagcac
     8281 gggaacatcc tcgatatggg cgcagggttc gatgaaatgc acgagcgctc ccatgcctat
     8341 cacccttccg tcccgcccgc cgctcagcgc aatcggctgt tgctgaatgc gataatgact
     8401 ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact tcgaattacc ccaggcagcg
     8461 agttaccctc tgctcgctga tcaattctct tgttttatat cccctggcac acagcacgtc
     8521 agttaatcag gaaactgtca tgaagagatc gatatcgttt cgtcccacat tgctcgcgct
     8581 cgtccttgcc acaaatttcc cggtttcgca cgccgccgta ccgaaagata tgctggtgat
     8641 tggtaaggcc gccgatccac aaaccctcga cccggcggta acaatagata ataacgactg
     8701 gacggtgacc tacccgtctt atcagcggct ggttcagtac aaaacggacg gtgataaagg
     8761 ctcaaccgac gttgaaggcg atctggcaag tagctggaaa gcgtctgacg atcaaaaaga
     8821 gtggacgttc accctgaaag ataatgctaa atttgccgat ggcacacctg tcactgccga
     8881 agcagtaaaa ctttcttttg agcggctact aaaaatcggc caggggccag cagaagcatt
     8941 tcccaaagat ttaaagattg atgctcccga cgaacatacg gtgaagttta cccttagcca
     9001 accattcgca ccgttcctct acacgctggc gaatgacggt gcatccatta tcaatccggc
     9061 ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc ctcgcgcaaa ataccgccgg
     9121 ttccggacca tttatgctga aaagctggca aaaaggtcag caattagttc tggtgccaaa
     9181 tccgcattac cccggcaata aaccgaactt taagcgagta tcggtaaaaa ttatcggtga
     9241 aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt gacattgccg atgcgctgcc
     9301 ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc aatgtggcag agtatccgtc
     9361 actgcgcgtt acctatctgt atctgaataa cagcaaagcg cctcttaatc aggcggatct
     9421 acgtcgggcc atttcctggt ctaccgatta tcagggcatg gttaacggca ttctgagtgg
     9481 taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg tggggctacg atgcgacggc
     9541 aatgcaatac aaccatgacg aaacgaaagc caaagccgaa tgggataaag tgacgagcaa
     9601 acccaccagc ctgacgtttc tctactccga taacgatccg aactgggagc ctattgcttt
     9661 ggcgacacaa tccagtctca acaagctggg tatcaatgtg aagctggaaa agctggcgaa
     9721 cgccaccatg cgcgacagag tgggtaaagg tgattacgac attgcgattg gcaactggag
     9781 tccggatttt gccgacccgt atatgtttat gaattactgg tttgagtcag acaaaaaagg
     9841 tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc gataagttac tgcgcaatgc
     9901 gcttgcgacc accgaccaga cgcagcgtac ccgggactac cagcaggcac agaaaatcgt
     9961 cattgatgac gctgcttatg tgtacctgtt ccagaaaaac taccaactgg cgatgaacaa
    10021 agaggtgaaa ggctttgtgt tcaatcccat gctggaacag gtcttcaata tcaataccat
    10081 gagtaaataa cgtgaccttc tggagtattt tacgccaacg ctgctggggg ctggtgctcg
    10141 tggtggcggg cgtctgcgtg attacgttta ttatctcgca tctgatccct ggcgatccgg
    10201 cgcggttact ggcgggtgac cgcgccagcg atgctatcgt ggaaaatatt cgccagcaac
    10261 tgggactgga ccagccactg tatgtacagt tttaccgcta cgtcagcgac ctgtttcatg
    10321 gtgacctggg aacatccatt cgtaccgggc gtccggtgct ggaagagttg cgtatatttt
    10381 tcccggcgac gctggagctg gcttttggtg ccctgctgct ggcactcctg attggcatcc
    10441 cgctgggcat actctctgca gtctggcgaa atcgctggct ggatcatctg gtgcgaataa
    10501 tggccattac cggaatctcc acacctgcgt tctggcttgg gctgggcgtc attgtgctgt
    10561 tttatggtca tctgcaaatt cttcccggcg gcggaaggct tgatgactgg ctggatccac
    10621 caacgcacgt taccggcttt tatctgctcg atgcgctgct tgaaggcaac ggtgaagtct
    10681 tcttcaatgc gttgcaacat ctcatcttac cggcattaac gctggcgttc gttcacctgg
    10741 gaattgtcgc tcgccagatc cgctcagcga tgctggaaca attgagtgaa gactacattc
    10801 gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt tttatgttat gcgctaccca
    10861 atgcgttgat cccatcgatt accgtattgg gtttggcgct gggcgatttg ttgtatggcg
    10921 cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg tgcatgggta gtaacatcaa
    10981 tacaggcgct cgacttcccg gcagtgatgg gctttgccgt cgtggtttca tttgcttatg
    11041 tgctggtcaa cctggtggtg gatttgctct atttgtggat tgatccgcgt atcggacgtg
    11101 gaggtggtga atgatgctaa gcgaggaaac gtccgccgta cgcccacaaa aacaaacgcg
    11161 atttaacggt gcaaaactgg tatggatgct gaaaggcagt ccgctcaccg tgaccggcgc
    11221 agtcatcatt gtattaatgc tattgatgat gattttttca ccgtggctgg cgacgcatga
    11281 tcccaacgcc attgatttaa ccgcccgcct tttgccgcct tctgcggcgc actggtttgg
    11341 caccgatgaa gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc agcaatcaat
    11401 tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac tcggatgtct
    11461 atccggtgtg cttggcggac gcgcagacgc catcatcatg cgcatcatgg acattatgct
    11521 gtcgattcct tcgttggtac tgacaatggc actggctgcc gctctcgggc cgagtttatt
    11581 taacgccatg ctggcgcttg ctattgtgcg aattcccttt tatgtgcgcc tggcgcgggg
    11641 gcaagcatta gttgtacgcc agtataccta tgttcaggcg gcgaaaacct ttggtgcgtc
    11701 gcgttggcat ctgatcagct ggcatatttt acgtaactcc ctgccgccgc tgatcgtgca
    11761 ggcatcgctg gatatcggta gcgcgatttt aatggccgcc acgttgggat ttattggcct
    11821 gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta gcgaatggtc gcaactatgt
    11881 actcgatcag tggtggtatt gcgcatttcc gggggcagcg attttgctta ccgccgttgg
    11941 gtttaatctc tttggtgatg gtattcgcga tctgcttgac ccgaaagcag gaggaaagca
    12001 gtcatgaccc aacccgttct ggacattcaa cagctgcatt tgagtttccc cggttttaac
    12061 ggtgatgttc acgcgctcaa caatgtgtcc ttgcagatta accgcggcga aattgtcggt
    12121 ctggtgggag aatccggctc aggtaaatca gtcaccgcaa tgctgattat gcgtctgcta
    12181 cagacgggca gttattgcgt acatcggggg cagatttcac tgctgggaga cgatgttctt
    12241 aacgctcggg aaaaacagct tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag
    12301 gaaccgatga ccgccctcaa tccgacactt cgaataggtc ttcagatgat ggacgtgatc
    12361 cgccatcatc aaccaataag tcgtcgggaa gccagagcta aagcgattgc cctgctggaa
    12421 gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct atccgtttga gctttcaggt
    12481 ggtatgcgcc agcgggtaat gattgcgctg gcattctcct gcgagccgca attgattatt
    12541 gccgacgaac cgactacggc gctggacgtc acggtgcagt tgcaggtact gcgtttgctt
    12601 aaacataaag cccgcgccag tggaactgcg gttctgttca tcagccatga tatggccgtg
    12661 gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg gaagcgtaat tgaaagcggc
    12721 gtgacggcag atgttatcca tcatccccgg catccgtata ccattggttt gctccaatgc
    12781 gcaccagaac atggagtacc acgccagcta ttacccgcca ttccagggac ggtaccaaac
    12841 ctcacccatt tgcctgaagg ctgcgctttt cgcgatcgtt gctatgcggc aggtgcacag
    12901 tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca accagcgctg cgcctgttgg
    12961 tatccgcagc aggaggtcat aagtgtctga cacgttatta acgttacgcg acgtccatat
    13021 caatttcccg gcccgtaaaa actggcttgg taaaactacg gaacatgttc atgccattaa
    13081 tggtattgat ttacagatcc gccgtggtga aaccttaggg atcgtcggcg agtcaggctg
    13141 cggcaaaagc accctcgcac agcttttaat gggcatgctg caaccgagcc acgggcagta
    13201 catccgttct ggctcacaac gcattatgca gatggtgttt caggacccgc tctcttcgct
    13261 caatccgcgc ttaccggtgt ggcgcatcat cacagaaccg ctctggatag ctaagcgtag
    13321 tagtgaacaa cagcggcgag cgttggcaga ggagctggct gtgcaggtgg gtattcgtcc
    13381 ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac gcatcgccat
    13441 tgccagagca ctctcttcgc agcctgacgt gattgtgctt gatgagccaa cctcggcgct
    13501 ggatatctcc gtgcaggcgc agatcctcaa tttactggta acgctacagg aaaatcacgg
    13561 gctgacttat gtgctgattt cacacaatgt ctcggtgata cgtcatatga gcgatcgggt
    13621 ggcggtgatg tatctcgggc agattgtgga actaggggaa gcgcaacagg tgttgactgt
    13681 tccggcacat ccatataccc gattattgct ggattccctc cccgccattg ataaaccgct
    13741 ggaggaagaa tgggcattac gtaaaacgga tctgccggga aaccgcacgt tgccgcaagg
    13801 ctgttttttc tacgaacgtt gccctctggc aacccacgga tgtgaagtcc ggcaatcatt
    13861 aacaataagg gaggacggac gtgagatccg gtgctggcgg gcgctgtaga gatgctaaca
    13921 acgcatcagg cattcacgcg cctgacgcgt catccggcaa tgctttacga tttcaactgg
    13981 taatcaagcg taatttccgc tttcagtacc tgagaaaccg ggcatcctgc ttttgctttc
    14041 tggatgatgc cgtcaaaggt agaggcatca atacccggca ccgcaacttc actcttcagt
    14101 gcgattttcg taatcgcaaa accggcatcc actttatcca gcgacacatc ggcggtggta
    14161 tcaatcgatg ttggcgtaaa tcccgcttcc cccagcatta atgaaagcgc cattgagaaa
    14221 catgcggcat gcgctgcgcc aatcagttct tcagggttag ttcctttttc gccttcaaaa
    14281 cgcgtgttaa atccatacgg ctgttggttc agcacaccac tctcggtgga tactgttccc
    14341 ttcccgcgtt tgatatcgcc ttcccagtgt gcctgacctt tcttatggat tgtcattgtt
    14401 gctctcctgt gggcttaaaa aggaaattat agactacgtg agaaacagca cgaggataag
    14461 caggatattc cgaataaaaa tccggcagaa atattacttc tgcacttaaa ttaaatttcc
    14521 acaatagata accgggaata tataacccaa gagatattgc attaatatta atcaactcac
    14581 cacaataata atacctatca atatcatgag gttaaaatat aaaaatcatc aaccaggact
    14641 aatcttaaca acgaagcggc aaatatttgc catgctgaat gtgctttata aatctgcgat
    14701 ccgtagcaga caccataaat acacagacac ggagaatcac tatgtttact tattatcagg
    14761 cagaaaattc aacagcagaa cccgctctgg ttaatgccat tgagcaaggc cttcgggcag
    14821 agcacggtgt tgtcactgaa gatgacattc tcatggagct gaccaaatgg gtggaagctt
    14881 ctgataacga catcctcagt gatatctacc agcaaacgat caattatgtg gtcagtggcc
    14941 agcaccctac gctttaaggt gctatgcttg atcggcaacc taatttaggg gtttagcacg
    15001 tgtttcttcg ctacggcgat gttgtcctta aaactagcaa caggattgag gagttaaaat
    15061 gaaatcgaac cgtcaggcac gtcatattct tggactggac cataaaattt ctaaccagcg
    15121 caaaatagtt accgaaggtg acaaatccag cgtggtaaat aatccaaccg gcagaaaacg
    15181 ccccgctgaa aagtaattca taaccatcag tcctcaatga cgattaaaca ccattgcctg
    15241 cgcaatggtg tttttgtttt tatctgctct atacttgggg ccgacgccct ggcggtaaag
    15301 caaagacgat aaaagcgtgc cagggatgga tattcaaaaa agagtgagtg acatggaacc
    15361 aaaaacaaaa aaacagcgtt cgctttatat cccttacgct ggccctgtac tgctggaatt
    15421 tccgttgttg aataaaggca gtgccttcag catggaagaa cgccgtaact tcaacctgct
    15481 ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag catggatcca
    15541 gtatcaggga ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca tccaggacac
    15601 caacgaaacc ctcttctacc gtctggtaaa caatcatctt gatgagatga tgcctgttat
    15661 ttatacccca accgtcggcg cagcctgtga gcgtttttct gagatctacc gccgttcacg
    15721 cggcgtgttt atctcttacc agaaccggca caatatggac gatattctgc aaaacgtgcc
    15781 gaaccataat attaaagtga ttgtggtgac tgacggtgaa cgtattctgg ggcttggtga
    15841 ccagggcatc ggcgggatgg gcattccgat cggtaaactg tcgctctata ccgcctgtgg
    15901 cggcatcagc ccggcgtata cccttccggt ggtgctggat gtcggaacga acaaccaaca
    15961 gctgcttaac gatccgctgt atatgggctg gcgtaatcca cgtatcactg acgatgaata
    16021 ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa cgctggccgg acgtgctgtt
    16081 gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta cttaaccgct atcgcaatga
    16141 aatttgttct tttaacgatg acattcaggg cactgcggca gtaacagtcg gcacactgat
    16201 cgcagccagc cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct tccttggcgc
    16261 aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc cagacccagc gcgaaggatt
    16321 aagcgaggaa gcagcgcggc agaaagtctt tatggtcgat cgctttggcc tgctgacgga
    16381 caagatgccg aacctgctac ctttccagac caaactggtg cagaagcgcg aaaacctcag
    16441 tgactgggat accgacagcg atgtgttgtc actgctggat gtggtgcgca atgtaaaacc
    16501 agatattctg attggcgtct caggacagac cgggctgttt acggaagaga tcattcgtga
    16561 gatgcataaa cactgtccgc gtccgatcgt gatgccgctg tctaacccga cgtcacgcgt
    16621 ggaagccaca ccgcaggaca ttatcgcctg gaccgaaggt aacgcgctgg tcgccactgg
    16681 cagcccgttt aatccagtgg tatggaaaga taaaatctac cctatcgccc agtgtaacaa
    16741 cgcctttatt ttcccaggca tcgggctggg tgttattgct tccggcgcgt cacgtatcac
    16801 cgatgagatg ctgatgtcag caagtgaaac gcttgctcag tattcgccgc tggtgctgaa
    16861 cggcgaaggt ctggtactac cggaactgaa agatattcag aaagtctccc gcgcaattgc
    16921 gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg gtgaaaacgt ctgccgaagc
    16981 tctgcaacaa gccattgacg ataatttctg gcaagccgaa taccgcgact accgccgtac
    17041 ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc tatttccctc tccctttttc
    17101 agatctcatc catactgggt agtggcgaat aaatctcatt tgcctcacct gctatgcaga
    17161 acatcatccg aaaaggagga actatgaagg ctgcagttgt tacgaaggat catcatgttg
    17221 acgttacgga taaaacactg cgctcactga aacatggcga agccctgctg aaaatggagt
    17281 gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg cgattttggt gacaaaactg
    17341 gggtaattct gggccatgaa ggcatcggtg tggtggcaga agtgggtcca ggtgtcacct
    17401 cattaaaacc aggcgatcgt gccagcgtgg cgtggttcta cgaaggatgc ggtcattgcg
    17461 aatactgtaa cagtggtaac gaaacgctct gccgttcagt taaaaatgcc ggatacagcg
    17521 ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga ttacgcggta aaagtgccag
    17581 atggtctgga ctcggcggcg gccagcagca ttacctgtgc gggggtcacc acctacaaag
    17641 ccgttaaact gtcaaaaatt cgtcccggtc agtggattgc tatctacggt cttggcggtc
    17701 tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa cgccaaagtg atcgccattg
    17761 atgtcaatga tgagcagtta aaactggcaa ccgaaatggg tgcagattta gcgattaact
    17821 cacgcaccga agacgccgcc aaaattgtgc aggagaaaac cggtggcgct cacgctgcgg
    17881 tggtaacagc agttgctaaa gctgcgttta actcggcagt tgatgctgtc cgtgcaggcg
    17941 gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag cctggatatc ccacgccttg
    18001 tgctggatgg cattgaagtg gtcggttcgc tggtcggcac gcgccaggat ctaaccgaag
    18061 ccttccagtt tgccgccgaa ggcaaagtgg tgccgaaagt cgccctgcgt ccgttagcgg
    18121 acatcaacac catctttacc gagatggaag aaggcaaaat ccgtggccgt atggtgattg
    18181 atttccgccg ttaagaggcc tttgctgcga gtgccagtcc tggtgtcgca gcatcgcact
    18241 ctctccgaca ttaaatggct aaatggatta ttgttccttc ctacgcaaca ataatgatag
    18301 tgttcctgat gaaggaacac tactgagagt aaagacactg atgaaaatgg ccaatcatcc
    18361 ccgcccgggg gacattattc aggaatcact ggacgaactt aatgtcagcc tgcgcgagtt
    18421 tgccagagca atggaaattg ctccctcaac ggcaagccga ttgctgaccg gaaaagcagc
    18481 tttgacacca gaaatggcga ttaaactttc cgtggtgatc ggcagttcgc cgcaaatgtg
    18541 gctgaatctg caaaacgcct ggagtctggc agaggcagaa aaaacggtgg atgtgtcgcg
    18601 actacgccgt ctggttacgc aataaaaaaa tggcgcttct taaagcgcca ttttcagctt
    18661 aaagttcaaa agagattata tcccttcttc actctctttt ttcgcttctg ccttctcgat
    18721 ttcacgatac cagcgcggat ggtgtttctt cgcccagcga cggctcacct tcccttcgat
    18781 catcccttta atcgaccctt tcacccagaa tgccatatac atatggatca ggatggcgtg
    18841 gatcaggatg atacccgcag ccgcgtggat cagcaggctg tagcgaacaa cctgcatcgg
    18901 gaagtactgc gcaaagtacg gacgccagat aatcaccccg gtcaccagca gcacgaaaat
    18961 catgctcatg atcgaccaga acatcatctt ttgcccggcg ttgtacttac cgacatccgc
    19021 cactttatgc tcattgcctt tcaatacttc gacaatgttc aacagccacg gaatatcttt
    19081 cttatccggg atgttgtgat gcacaaaacg cacaaacata aacatcagtg cgacgaaaat
    19141 cgcaatgccg aaaaacgggt gcaaaatgcg tcccatctgc ggcgtaccga aggtttgcgt
    19201 cagccattgc agcgtcggga agaagaacga aatcccggac agcgccacca ggaagaagca
    19261 aatcaccacg gtccagtgac aggcgcgatc aataaatttg gtgcgcacaa tcattttcga
    19321 cttactcatg atgatcctcc tcgtcatcgt ccacttcctt attcgggcca ataccgatgt
    19381 agtggaaaat caacccggca aaagtggcaa taaagccagc cgctgccagc ggtttcaacg
    19441 cgcctttcca caggcttacc gaggtgtcga tcttcggatc tttcggcaga ccgtgataca
    19501 gctccggctg atcggcgtga tgcagcacgt acataacgtg cgtaccaccg accccttccg
    19561 ggttgtagac gccagcatgt tcgtaaccac gcgctttcag tttcgccacg cgctgttccg
    19621 ccagctccag catctccttc ttggtgccga agtggatagc cccggtcgga caggttttca
    19681 cacaagccgg ttcctggccg acgctgacgc gatcgacgca gagcgtgcat ttatataccc
    19741 ggttatcctc tttgttgagg cgcggaatat taaacggaca cccggcaatg cagtaaccac
    19801 agccgatgca gttttccgac tggaaatcga caatcccgtt agcgtactga atgattgcac
    19861 cagcagacgg gcacgccttc aggcagccgg gatcttcaca gtgcatacag ccgtctttac
    19921 ggatcagcca ctccagcttg ccgttctgtt cggtttcgct aaagcgcatc accgtccagg
    19981 acttggcgct cagatcggcg gggttatcgt aaaccccgac gcagtgcccc acttcatcac
    20041 ggatgtcgtt ccactccgaa cacgccacct gacaggcttt acagccgata caggtggaaa
    20101 cgtcgataag ttttgcgact tctgctttgt aatcacgcac ctgagaaggc ggcgtgatgg
    20161 agttagttgc ggaccttttg ataatgtcct gcgtttccat agccatctgt tcgccccctt
    20221 acgccttctc gatgttgact aagaacgctt tatattccgg cgtttgcgag tttgcatcac
    20281 cgacattcgg cgtcagagtg ttagcgatat aacctttacg cgcgacaccc tcaaagcccc
    20341 agtggattgg aatacccacc gtttcaacct gctgaccatt tacattcagt ggtttcagac
    20401 gacgcgttac cacagccacc gcgcggataa agccacgctt gcttgagaca gtgacacgat
    20461 cgccattatt aatgcctttc gccgccgcca gcgtttcgct gatttccaca aactgttccg
    20521 gctgagcaat tgcgttgagc aatgcgtgct tggtccaggt gtggaagtgc tcggtcagac
    20581 gataggtcgt acccacatac gggaactgct cttttttacc catccgcagt gcgtcttgtt
    20641 catacagacg aacaaccggg ttagacacca cgttcggatg cagcgggtta gtgcccagcg
    20701 gcgtttcaat cggctcgtag tgttccggga acggaccttc cgccattttg ttgatggcaa
    20761 acaggcgtcc catcccttcc ggctgcatga taaacggccc ggttggcgta cccggtgcgg
    20821 cattgccgaa gtcaggaata tcgttacccg tccacttgct gccgttccac tggatcagca
    20881 tccgtttcgg atcccacggt ttaccgttga tatccgccga agcacggttg tacagcacgc
    20941 gacggttgag cggccacgcc caggcccatc ccagcgtatt ccccagaccg gacgggtcgg
    21001 agttatcgcg gttagccatc tggttgccct gctctgtcca gctaccggtg tagatccagc
    21061 aagaagatgc ggttgtaccg tcatcacgca gatgcgcaaa gctactcagc aactgacctt
    21121 tcttcgcaat cagcacgcca ttagcatcat agagatcttc cagcgcatag ccgttgttct
    21181 ctttagctac ttcgtcagat tgcggttcgt gcggctgctt gtagttccag ctcatcttca
    21241 tcagcggttc tacgccttta ccaccttcgg cctggtacag ctcgcgcagg tgatggtaga
    21301 tacccgccag aatttcaccg tcgttacgcg cttcgcccgg cgcatcctga cctttccagt
    21361 gccactgcag ccagcgaccg gagttagcaa tagaaccatc ttcttcagca aagcaggtcg
    21421 aaggcagacg gaatacttca gtctgaatag acgccggatc gacatcgttc gactcgccgt
    21481 ggttctgcca gaaggttgag gtttcagtca ccagcggatc gataaccacc atgtacttca
    21541 gcttgcttag gcagctcacc actttgtttt tgtccgggaa ggacgcaacc gggttaaagc
    21601 cctggcagaa ataaccggtg actttgcctt catccatcat gttgaaatac ttgatgacgt
    21661 cgtaggtctg gtcccacttc ggcagccagt catagcccca gttgttctct ttctgcgcgg
    21721 catcgccata gaaagatttc atcaggctaa cgaagaactt cggatagttg ctccagtagt
    21781 tcacctgacc agccagcgtc gctttcggcg tgttcgcttc cagatacgac tgcaaatcaa
    21841 cctgtttttc tgacggcagc gtcagataac ctggcaggct ggtagagagc agacctaagt
    21901 cagtcagacc ctgaatgttg gagtgaccac gcaatgcatt cacgccgcca ccggccatac
    21961 ccatgttacc gagcagcaac tggatcatcg ccatagtacg gatgttctgc gcacccacgg
    22021 tgtgctgcgt ccagcccagc gcgtacagga aggtggttgt gcgatccggt gcgctggtgg
    22081 aggccagcac ttcacacact ttcaggaagt cggcttttgg cgtaccacag atgttttcaa
    22141 caacgtccgg cgtgtagcgg gaaacgtgcg ctttcagcag gttccacaca cagcgcggat
    22201 gagtcagtgt ttcatcgcgt ttcgcatagc cgttttcatc gaactgatag ttccaggacg
    22261 atttatcgta ttgccgtttt tcagcgtcgt agccgctgaa cagaccgtct tcgaaagcaa
    22321 aatcatcacg caccagcagg ctggcgttgg tgtaatgctt aacgtattcg gcgttgattt
    22381 tgtcgttttc gatcaggtag cgcaaaacgc cagacaggaa cgtaatgtcc gtaccggaac
    22441 gaataggtgc gtaaatatcc gccacagaag cggtacgcgt aaaacgagga tcgacaacga
    22501 tcaaggttgc atcgttgttg tttttcgctt ccatcgccca gcggaaaccg acgggatgcg
    22561 cttcagcagc gttaccgccc attaccatta cgacgttagc gtttttgata tccacccagt
    22621 ggttggtcat cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc
    22681 agacgcgcgc ctggttgtct accgccagca tcccgaggga gcgggcaaat ttctgtgtca
    22741 gcatcccggt ttcgttgctg gcaccggagg cacacagcat accggtagaa agccaacggt
    22801 ttaccgttac gccctgctcg ttcttttcaa taaagttagc gtcacggtca gctttcatca
    22861 gctttgcaat acgggagaat gcttcttccc agctaatgcg ctgccatttg tcagaacctg
    22921 gcgcacgata ttccgggtag cgcagacggt tttcgctgtt gacgtaatcc agcaaaccgg
    22981 ccccttttgg gcacagcgca ccacggctta ccggatgatc cgggtcacct tcaatgtgat
    23041 aaatcgcttc tctggcgttt ttcgcgccat cacccaggct atacatcaat agcccgcaac
    23101 ctacggaaca gtatgtgcag gtgttacgga tctctttagc gcgtaataat ttgtagtttc
    23161 gcgcctgagc cagtgcttgc ttcggggcaa agcccaatgc cgctaccgtt gttccagcca
    23221 taccgcccgc gcagatttta aaaaattgtc tgcgactgac gtccattgct ttcctctttt
    23281 ttcaggggta ttactgcgcg aggaaactaa cagtaaaatt ctgaatcttt ttgcgcgaaa
    23341 tcaaaaagaa cacgtgtttg ccacaaaata atccgcctgc gcaaattgac ctacctcaat
    23401 agcggtagaa aaacgcacca ctgcctgaca ggccagttaa aaaaatgcta taaaattcag
    23461 cttaattttt aacggcaaga gagacaaaac agcgagcatg acacgacaaa aagcaacgct
    23521 catagggcta atagcgatcg tcctgtggag cacgatggta ggattgattc gcggtgtcag
    23581 cgaggggctc ggcccggtcg gcggcgcagc tgctatctat tcattaagcg ggctgctgtt
    23641 aatcttcacg gttggatttc cgcgtattcg gcagattccg aaaggctatt tactcgccgg
    23701 gagtctgtta ttcgtcagct atgaaatctg tctggcgctt tccttagggt atgcggcgac
    23761 ccgtcatcag gcgattgaag tgggtatggt gaactatctg tggcccagcc tgacaattct
    23821 ctttgccatt ctgtttaatg gtcagaaaac caactggctg attgtaccag gattattatt
    23881 agccctggtc ggcgtctgct gggtgttagg cggtgagaat gggttacatt atgatgaaat
    23941 catcaataat atcaccacca gcccattgag ttatttcctg gcgtttattg gtgcgtttat
    24001 ctgggcagcc tattgcacag taacgaataa atacgcacgc ggatttaacg gaattaccgt
    24061 ttttgtcctg ctaacgggag caagtctgtg ggtttactat tttctcacgc cacaaccaga
    24121 aatggtattt agcacgcccg tcatgattaa actcatctct gcggcattta ccttaggatt
    24181 tgcttatgct gcatggaatg tcggtatact gcatggcaat gtcaccataa tggcggtagg
    24241 ctcgtatttt acgcccgtac tttcctcagc gcttgcagcc gtgctgctca gcgccccgct
    24301 gtcgttctca ttctggcaag gcgcgctaat ggtctgcggc ggttccctgc tctgctggct
    24361 ggcgacacgt cgtggttaaa ataattgccg ggtctacggc ccggcagtta atcagataaa
    24421 ttccgacaat attaaaaaat a
//
LOCUS       DAZFJF010000071        24223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFJF010000071 DAZFJF010000000
VERSION     DAZFJF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.071"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..3867)
                     /gene="hrpA"
                     /locus_tag="ACHKAU_003038"
     CDS             complement(<1..3867)
                     /gene="hrpA"
                     /locus_tag="ACHKAU_003038"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO5532055.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDK"
     gene            4068..4673
                     /gene="azoR"
                     /locus_tag="ACHKAU_003039"
     CDS             4068..4673
                     /gene="azoR"
                     /locus_tag="ACHKAU_003039"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO5532056.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4724..6040)
                     /locus_tag="ACHKAU_003040"
     CDS             complement(4724..6040)
                     /locus_tag="ACHKAU_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO5532057.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYLLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(6030..7787)
                     /locus_tag="ACHKAU_003041"
     CDS             complement(6030..7787)
                     /locus_tag="ACHKAU_003041"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO5532058.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(7803..8699)
                     /locus_tag="ACHKAU_003042"
     CDS             complement(7803..8699)
                     /locus_tag="ACHKAU_003042"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5532059.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8699..9304)
                     /locus_tag="ACHKAU_003043"
     CDS             complement(8699..9304)
                     /locus_tag="ACHKAU_003043"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5532060.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9475..11781)
                     /locus_tag="ACHKAU_003044"
     CDS             complement(9475..11781)
                     /locus_tag="ACHKAU_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO5532061.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(11845..12705)
                     /gene="pdxI"
                     /locus_tag="ACHKAU_003045"
     CDS             complement(11845..12705)
                     /gene="pdxI"
                     /locus_tag="ACHKAU_003045"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO5532062.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(12936..13526)
                     /gene="paaY"
                     /locus_tag="ACHKAU_003046"
     CDS             complement(12936..13526)
                     /gene="paaY"
                     /locus_tag="ACHKAU_003046"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO5532063.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(13508..14458)
                     /gene="paaX"
                     /locus_tag="ACHKAU_003047"
     CDS             complement(13508..14458)
                     /gene="paaX"
                     /locus_tag="ACHKAU_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO5532064.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(14559..15872)
                     /gene="paaK"
                     /locus_tag="ACHKAU_003048"
     CDS             complement(14559..15872)
                     /gene="paaK"
                     /locus_tag="ACHKAU_003048"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO5532065.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(15899..17104)
                     /gene="paaJ"
                     /locus_tag="ACHKAU_003049"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(15899..17104)
                     /gene="paaJ"
                     /locus_tag="ACHKAU_003049"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO5532066.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGPGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(17104..17526)
                     /gene="paaI"
                     /locus_tag="ACHKAU_003050"
     CDS             complement(17104..17526)
                     /gene="paaI"
                     /locus_tag="ACHKAU_003050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO5532067.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(17516..18943)
                     /gene="paaH"
                     /locus_tag="ACHKAU_003051"
     CDS             complement(17516..18943)
                     /gene="paaH"
                     /locus_tag="ACHKAU_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO5532068.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(18945..19733)
                     /gene="paaG"
                     /locus_tag="ACHKAU_003052"
     CDS             complement(18945..19733)
                     /gene="paaG"
                     /locus_tag="ACHKAU_003052"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO5532069.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(19733..20500)
                     /gene="paaF"
                     /locus_tag="ACHKAU_003053"
     CDS             complement(19733..20500)
                     /gene="paaF"
                     /locus_tag="ACHKAU_003053"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO5532070.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(20497..21567)
                     /gene="paaE"
                     /locus_tag="ACHKAU_003054"
     CDS             complement(20497..21567)
                     /gene="paaE"
                     /locus_tag="ACHKAU_003054"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO5532071.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(21575..22072)
                     /gene="paaD"
                     /locus_tag="ACHKAU_003055"
     CDS             complement(21575..22072)
                     /gene="paaD"
                     /locus_tag="ACHKAU_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO5532072.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(22087..22833)
                     /gene="paaC"
                     /locus_tag="ACHKAU_003056"
     CDS             complement(22087..22833)
                     /gene="paaC"
                     /locus_tag="ACHKAU_003056"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO5532073.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(22842..23129)
                     /gene="paaB"
                     /locus_tag="ACHKAU_003057"
     CDS             complement(22842..23129)
                     /gene="paaB"
                     /locus_tag="ACHKAU_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO5532074.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(23141..24070)
                     /gene="paaA"
                     /locus_tag="ACHKAU_003058"
     CDS             complement(23141..24070)
                     /gene="paaA"
                     /locus_tag="ACHKAU_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO5532075.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
BASE COUNT         6055 a         6658 c         5659 g         5851 t
ORIGIN      
        1 cttatctgaa atcggataag gcgtaccaag ttgttgagcg aagtaactaa cgcgcaactc
       61 ttctatcatc caacggatct ctttcacgtc ttcatcctca cgacgtgcgg gcggcagttt
      121 gttgatccat tgctgccacg cctgctggac gttttcgact ttcagcatct gagcacggtc
      181 gcgatgtgga tcaaccgcca gtttttccag ccgtttttca atcgcctgca aatatcgcag
      241 cgtgtcgccc agccgtttga agccgttacc agtgacaaaa ccgcgatata ccaacccgcc
      301 catctgcgct ttaatgtcag aaagccccag tgccatggtc atatccaccc gccctttcag
      361 acgtttgttg atattgaaca ccgcagtgag gatttgctcg acctgcttcg caatatccac
      421 caccgtgtca ttcagttcgg cgcgcacttt ttcatgcagc gcagcaaagc cttcttccgt
      481 ccagaccggg ccaccattgg cgtcgatcaa ttgatccaca ccgcaggaga tacagtcgtc
      541 gatcagctcc agcactttgc catacgggtt aaagtacagt cccagcttgg ctttgttcgg
      601 taacttttca tgtaaatatt tgattggcga tggaatattc agcagcagta gacggcgaag
      661 accgttccac attgcctgct tttgctccag cgggttatca aacagtttga tcgccacact
      721 gtcgcgctca tccaccagcg ccggccacgc cttcactttg tagttgccac gcttctgttc
      781 gtagctttcc ggcagctgac caaaactcca gatatgtaag ccgctctgct cgataccgtc
      841 atccgccacc gcagatagcg tttcctgcac tttgcctttc agcgcatctt tcagatcttg
      901 tagcgagcgc ccttctttta gcttcttgtt tttgtcatcc accacgcgga aggtaatttt
      961 caggtgatca ggcacctgat cccagtgcca gtcttcgcgg tcaacggtaa cgccggtcat
     1021 ccgccgtaac tcgcgctcaa ggctgtcgag caacggtaac tccagcggtt tgacgcggcc
     1081 taaaaacgct tcggcatagt ttggcgcggg tacaaaatta cggcgtaccg gtttcggcaa
     1141 cgatttaatc agagcaatca ccagttcgcg gcgcagaccg gggatctgcc attcaaaccc
     1201 gctttcctca acctggttaa gtaacggcag cggaatatgt acggtcacac cgtcagcatc
     1261 cgcgccgggc tcaaactgat agctcaaacg cagcttgaga ttgccctgat gccagaagtt
     1321 cgggtaatcc agcttgctga ttttttctgc gccctctttg atcaacatgc ttttttcaaa
     1381 gttgagcaaa tcaggggttt cgcggctgac ttttttccac cagctgtcga agtgacgagc
     1441 ggagattaca tcgtggctga tgcgctggtc gtagaactca aacaacgttt cgtcatcaac
     1501 cagaatatcg cggcgacgtg atttgtgttc cagctcttcc acttcggccc gcagtttcag
     1561 gttttcacgg aagaatgcgt gacgcgtctg ccagtcacct tccaccagcg catggcgaat
     1621 aaagagttca cggcataacg ccggatcgat ctggctgtag ttgaccttgc gcgcggcaac
     1681 aatcggcaaa ccataaacag tgactttttc cgttgccatc accgcgccct gcgcccgttc
     1741 ccagtgaggt tcgctgtagg tgcgtttaat caaatgctga gcgacaggct ccacccattc
     1801 cgggtcgata cgcgcagcaa tgcgccccca caggcggctg gtttctacca gttccgccac
     1861 cattacccat ttcggcggtt ttttgaataa accagaaccg ggaaagatgg agaaacgcgc
     1921 gttacgtgcg ccggtatatt cttgtttatc ggcatctttc atgccgatat gggaaagtaa
     1981 acccgtcagc aacgcaatgt gaatttcgcg atactccgcc ggttcgctgt taaccggaat
     2041 gccaagttct ttcaccacct ggcgcaactg ggtgtagata tcctgccatt cgcgcacgcg
     2101 cagatagttg agataatcga tacgacacag gcgacggaag gcgttggaag aaagcgcctt
     2161 ttgctgctcg ccaagataat tccacagatt cacaaacgcg aggaagtcag actctttgtc
     2221 gtggaagcga cgatgttttt cgtccgatgc ctgctgttta tccatcggac gctcgcgcgg
     2281 atcctgaatg gaaagcgcgg acgtgataat catcgcctca cgcacgcagc catgtttttg
     2341 cgcttccagc accatacgcg ccagacgtgg atcgacaggc aactgcgaga gctggcgacc
     2401 gagcggcgtc agtttatagg cgctggcctg ttcatccgtg gtgatcgcgc ccagttcttc
     2461 gagcagacgc acgccatcct ggatattgcg tttatccggt gcttcgacaa acgggaacgc
     2521 agcgatatcg cccagcccca gcgcggtcat ctgcaaaata accgaggcca ggttggtacg
     2581 cagaatctcc ggatcggtaa actccgggcg cgaaaggaaa tcgtcttcgg aatagagacg
     2641 aatacagatc ccttcggaca cacgcccaca gcggccttta cgctgattgg cagacgcctg
     2701 cgaaatgggt tcaatcggca aacgctgcac tttggtgcga tagctgtagc ggctgatacg
     2761 tgctgtaccg gggtcgataa cgtatttaat ccccggtacg gtcagcgagg tttccgcgac
     2821 gttggtcgcc agcacaatgc gccgtccgct gtgcgactgg aacacgcggt tctgttcgct
     2881 gttcgaaagc cgcgcataaa gcggcaagat ttcggtatga cgcagattta gcttgttcag
     2941 cgcatcggcg gtatcgcgaa tttcccgctc accactcata aagatcagaa tgtcgccagg
     3001 gctttcctga ctcagttcgt ctacggcgtc aaaaatcgcc tgcaactgat cgcgctcggt
     3061 gtcgtcggct tcttcaacaa tcggtcgata gcgcacttcc accggatagg tccgaccgga
     3121 gacttcgata atcggcgcat tattaaagtg gcgcgaaaag cgttccgggt cgatagtcgc
     3181 ggaagtgata atgattttta ggtcaggacg ccgcggcagc aactctttca aatagccgag
     3241 cagaaaatcg atattcaggc tgcgttcgtg tgcttcgtca ataatgatgg tgtcgtactg
     3301 catcagcagg cggtcttgct ggatctccgc cagcaggata ccgtcggtca tcagcttgac
     3361 catcgtgtta tcactaacgt gatcgctgaa acgcactttg taaccgatgc aaccgcccgg
     3421 ctccgttttc agctcttccg caatacggtt cgccaccgtt cttgccgcca ggcgacgcgg
     3481 ctgggtatgg ccgatcagcc ctttaatccc gcgccccagc tccatacaga ttttcggtaa
     3541 ctgagtcgtt ttaccagaac ccgtttcccc ggcgacgatc accacctggt gatcacgaat
     3601 cgcttcgaga atgtcctgtt ttttctgact aaccggtaaa ttgtcaggat aagtaatttc
     3661 cggtcgtgcc gcttcacgca gcaggacttt ccctgccgcc tggtcaatct ctttcgccat
     3721 ctcctggaaa atggcctgtt gtgcatcagg atttttaacc ttcttaacac cgtgcagacg
     3781 ccgagaaaaa cgcagtctgt cgcgcagcat cagcgaatcc agccgctgct gcaaggccgt
     3841 aaaggtcaat ttttgttgtt ctgtcataac gttagtgggc agtagtctgc ctgattaatt
     3901 ttcttttaaa tgatagatat agagtaccac atcgcggctt tctatgcgtt gttcaataaa
     3961 ttcgaacata ggcttcgatt tattgcgcta tctctgtggc aggattgtga ataaagtgtc
     4021 agcaagcaac ggggcatcgc ccattcaaac atctataagg aaacaccatg agcaaggtat
     4081 tagttcttaa atccagcatc ctggcagggt actctcagtc taatcagttg tccgattatt
     4141 ttgttgaaca atggcgcgaa aagcactccg ctgatgaaat caccgttcgc gacctggctg
     4201 caaatccgat tccggtactg gatggcgaac tggttggtgc actgcgtccg agcgatgcgc
     4261 cgctgactcc gcgtcagcag gaagctctgg cgctttccga tgagctgatt gccgagctga
     4321 aagcccacga cgttatcgtt attgcggtac cgatgtataa cttcaacatc tcaactcagt
     4381 tgaaaaacta ttttgacctg gttgcacgcg caggcgttac tttccgctat accgagaacg
     4441 gtccggaagg tctggtaacg ggtaaaaaag ccatcgttat taccagccgc ggtgggatcc
     4501 acaaagatgg accaacggac ctggtgacgc cgtatctgtc cacgttcctc ggctttatcg
     4561 gcattaccga tgtgaaattt gtcttcgccg aagggatcgc atacggtccg gaaatggcag
     4621 cgaaagcgca gtctgacgcg aaagcggcca tcgacagcat tgttgctgca taaccggaca
     4681 cgatcttgat gtggactgta gttcagtcca catctcaatc cacttacctg ttttcccata
     4741 atttcagcat cgctttgtga tcttccttaa gcacaatatg cgaacgtctg gctcgaatat
     4801 aactaatcgc ttcatcaacg gttttacagt gtccgtaaca taacaaccat gccgccacta
     4861 ccagcgcact gcgcgataat cccaacgcgc aatggaccag aacgctgcct tgctcttcgc
     4921 gtaatgtttc cagcatcgcc acggcctgtc ggagctcccc ctcttccgga accaccagat
     4981 ccagcatcgg tacacagaaa tagagtcgat cttttgtcgc tcgtccccga gggaattcaa
     5041 aggtgacgtc cagaaccgca ttctgtgccg gaatatgtcg tggaaacgcc cctaaataaa
     5101 caccagcagt aattttgctc accggctcca ggcgacgaca aaaccaacgc atagacagcc
     5161 acatcccgat gcgccagggc aatgtcagcc agtaaacggc gggcgggagt ttcccctgac
     5221 tatctttccc tgttgttatc gcgccaagcc caccgtaccc acggccaata atgagtagcg
     5281 ataatactgg ccaacataac cagactgacc accataactg aatcatcacc attagctcca
     5341 tcaacacaat gcacgagccc gcgcctacga catatggcag tgctattttg attcgacgtt
     5401 gatcaggttt ctgataattc caacgacgat cgacgggcac catccagtca atcaacatac
     5461 ctaccgccag ccctgtgatg acatcaataa aatgatgctg ccaggtcgtc agagtagaaa
     5521 tagcgatgag taaaaaccat ccgccacaga ctttacgcca cctctcagcc agatgctgac
     5581 gaaagtgacg ccaaagtagc cagcagagaa taatatgcag cgaaggagac tggttataag
     5641 gcagatcaaa cagttcaagt tgcgaaaata gccatcccgt caccccactc acttcaggac
     5701 ggataaaact aaacttcagc agatagagca aaaaaccgca gcaggccatt accgttgcca
     5761 gaataagccg gtggacaagt cggcgctgtt cgaatgtcgt gctacaaacg aacagcgaaa
     5821 atccatataa aagatccaga ctccagtaag gaacaatagt ccagggaaga aaagggatcg
     5881 ccgtttccca accgaatacc tgactgggga tatcatggct gttaaggtcc tgaaccgcgg
     5941 tgaactgatt aagagatcca taggtgaaga agaaaaacgg ggccagcaac aataaccagc
     6001 cagcgccttg tagcaatacg ttccgacgtt cagttatcac gacgcaccgc catcgaaacc
     6061 gtaaaaatac cccactcatc aatccgttgt gtgcatttat caaatccggc atcacgcacg
     6121 agtgaatcca tctccccttg cgaacgcacg cgcattaccc acggtttacc atctttatga
     6181 ctggttaaca ccccggcaat catctccagt tgagggtgcc acggctgccc ggtgtagatg
     6241 agaatgccgc ccggttcgat ggcattggca agacctgcga gtgagttttt tacctgctca
     6301 ttttcgggaa aaagctcata caggccagag acaatcgcca gcgtaggccg cggagttaac
     6361 gcgctgagtt cctccgggtt aaacgcatcg ccctgttcaa aacgcacccg cccagacatt
     6421 ccccgttggg caatcatcgc ttgcccctgt gcaacattta actcactgta atcacgtaac
     6481 aaaatatcgc ttacggcagg ctcgtttgcc agcgcatcca gtacatagcg cccatgccct
     6541 gcggcaatgt caaccacgcg gacggctaaa cctttggcgt ggagatcggc aacggcttgt
     6601 ttaatcagta tttgtaaatg ggttttacgc tggcgaatac cgcgccagcc aacactgttc
     6661 aggtagattt tgtcaattaa gcgcccgaat gcgttactgc cttgcggttg attttgatag
     6721 acataatcca gggaactgcc tgaatcaaag ccggtgctca ttccgagatg caggcccgca
     6781 gagtgcgccc cgaacaattt catcgccttt cgcataaagc gatacgccaa atcaaccggc
     6841 gataatggca cgggtccacc tgaaaggagc cgccagcgat ccgctgatgg tccagtgcgg
     6901 tcttcatgct gataatcaaa tttttgtgac ttgttagcat ataaacgact aataaagctt
     6961 tgcatttttt caaatgcctg cgccctgttc tcttcaccca acgtgtcgtg ataaaagcct
     7021 ggcagcagat gcagctcttt cagagggcta cgtaatctct gataaaaatc aatctgcggt
     7081 tggcgatgca ccacatagtc atcgcctgat atcagaagtt gcgtggggag cgtaatcgcc
     7141 gccgcatctc taataatacg ttcagacgtt ttgtagagat cgagcaagat attaacggca
     7201 atcgcccgtg tgatcagcgg atcattattg aaactcgcca cccgttgccg atcgtgggtc
     7261 aaatagcgtc ctttcacata ggaattaata aaaaacagac cacgcagacg atgccataac
     7321 gccagcgcag gacgtgccag cggtacatac aatttaacct taaaggccgg agaagccagc
     7381 accagcccgc gaattgcagg cgcataatca tgaacccatg tggcaaccag cactgcgccg
     7441 acgctttgcg cgatcactac cacctcatcc agtccgacct ggctatcgct ggcagcaaaa
     7501 cgaacaaatt catcgacatc ctgcactgaa cgcgcaagag atgggctata accacgcggc
     7561 cccgaagttt gtccatgccc tcgggcatcc caggcataaa aaacagtatc tggcatcgcc
     7621 agttcatcaa cgagatgttg tagacgacca gaatgttcat gaccgcgatg aaataagacg
     7681 atgacctttt tagccccggg ctgtaaagcg ggccagtgcc gataaaacaa cgctgtatta
     7741 tcactgctgg taaaaaaatg ttccccatgg atgcgtgaat tttccatttt ctctattttc
     7801 cttcagtaac agcagtagcg aataaaataa aaaaataccg gagcggtgaa aattaatgag
     7861 tcaatcctgt caaggagtcc gccatgtcct ggtaatagtt tcccactatc tttaacacca
     7921 atatctcgtt tgatggctga catcacgaca tcgccgcaaa aaccactaat accaattaac
     7981 aaacccgcta ataatgcctg taatgtattt agcggtgtca gcaacggtcc aataatcagt
     8041 gacgcaatca tggtggtgat tacgcccccc accagacctt ccagcgtttt tcccgggctg
     8101 actttgggga ccacttttct tctgccgcag gatttccccc ataaatactg tgcaatatcg
     8161 tttgactcgg ttaatgccaa aagaaataac accagtaacg ccccgccctg gatatttgtg
     8221 gttggcaaca ttaataacca ggcggcatga ctcaaggcga aaacggtcat tatccagccc
     8281 caaaaaatgg cgctcacggt atgcagaaaa ccggaggggt cccccataaa aacttgcccg
     8341 gtggctaata ttagaaaccc agccaaaggt ataaataata gatacagctc aaagcaatta
     8401 aaaccgataa acaaatagtt aagaggaata acccaatata accaccgcgg aaaatgtaca
     8461 gatataagcg tacaatattc tttcaatgcc agaaaactta tcagcgcaaa aaacgtcaac
     8521 gtcatccagc gtggacctga aatcaccagt gaaaaacaaa tgacgatgcc ccaccaggta
     8581 cgaatacgta atgtaacttc atcctctttt ctctccggta accgccatag cagaaaatgg
     8641 tttatcagcg ttgctaaaat taacaacgcg aaaagtgttg ccagagattt ttccagcatt
     8701 atctttcagc gctcataaga agcacactcc gacaacgatt aatcgcagtc cagagaagca
     8761 gaattgaagc aatgctccat aaaagattat tccactgcat ccactgtgga taaatggcaa
     8821 cggccagacc ccacagacca aatattaacg cgcgatcgct tttgccaaat ggaccggcat
     8881 aactgcgaac accgttaatc gtctgcgcga gtaaaccgca aaactcggtc aatatggtgc
     8941 aaaataacat gagtatcacg agtgatgcgt tactttccgg taaaaataaa aatggtaaat
     9001 agagcgcaat atcggaaata acatcaccag tctcattcaa aattgcccca agccgtgttt
     9061 gctgattgca ctcacgcgcc aacatgccat ccagcgcatt gagcgccata cggataaaaa
     9121 gcacgatggg caatagcata aagaggatgg gttgtgccac caacatcaac agcaatccgg
     9181 taagaagaga aagcgccagt gcagcaagag tgatgtgatt cgctgtaacg tggtgcttgt
     9241 aaagccaaaa catcgtcggc cttaacagcg actgaaagag cggttttatc tggtagagcg
     9301 tcattatcaa tccttgataa ttcggtttgc gctatggtgc atcaattccc ctgacggaga
     9361 tatcgcaaca cgccgcttca ttaaaaattc agaatatatc aaggtattgg ggactcactg
     9421 tcaggaataa ttatccccaa ttaatcaaag ggttaagaaa tattacgttg tggatcaata
     9481 caactcatca agttttctca tcgcttgttc ccgtattgcg cgcgtcttac cgttaagcgc
     9541 cagctggcgg actaaatcca gttcagggtt aacaagctct aacgccagca aaggatcacg
     9601 tttcagcctc gcctttaata catcaactgg aaaacgggga ttattcatcg ccacaatcca
     9661 ccactcggga tcaatttctg ccgccagtac tgcaggcggt gtgtggcggt tgttaagtgc
     9721 gccgaacttg aaaaatgtga gattgctttt cgccagtgta ctccaccata tacgatccgg
     9781 ttgttttgcc agtacatcca gttgtgctaa cgcatcgggg tcgagtgcat aatagacgcg
     9841 taatgcttca tcaacctgcg cacgggagct gttttcctgc ctcagaaaaa acttcgccca
     9901 aatatcattg cgtggcgtat tttgctgtaa ttccttgagg acaacagcgg gtaaattatc
     9961 ccaactaact aatgcactct ctaaaaagtc ccagtcttcc aaacgtatca gggcggtggc
    10021 atttaatagc atctgatcat caacttcacc gtggctgata agctcagaca aaatcatatc
    10081 agcgctggtt tcatacgcag ctgaatctgc tttcggatta tttttccgat gcgataaagc
    10141 acgttgccaa attttttgtt tcatcgacgt gctcagatac tgatttttcg ccagattact
    10201 ccacccctca ggatcgttga aacgtgtcaa taaccgttgt agagcagcgt cagagtgaat
    10261 tacctgcata tcccgacagc aaaagtaggc aggggatttt ttctccatcc ttttaacaaa
    10321 attaaactgt aagttatctg gcaatgctaa aaataacaca cgtattagat catcgtcatt
    10381 ctcgaatttt tggtataaat aggctgcaac tttgtcactc tcttgtggcg tcatccgcaa
    10441 taattgacca tggtgtttca tctctgccag cttgaacgct aagcttgtga caacattgtt
    10501 tgatgaatcg ttcgccaact gcattgcctg attgtaggtg agttgtcctc cagcaccgac
    10561 ctgacgaata gaggaatcat cactgcggat atagccatca ataatggatt caggcaataa
    10621 ccacgcatct ttataaagct cctgaatgat catatcccaa tctcttttcg ccagagtcat
    10681 tacgatatcc agagggagat tttcacgtag cgcaagattc aacggaccga caccatcagg
    10741 tagacgactc attcgcgtca ttatttcgac agagagcggt tcacggtcga gcaaccccag
    10801 aacaacaggc tggtaaccca attgctgatc ggtcaactcc agaatctcat tgtcaggcat
    10861 atatctcgcc atactttcaa tcgtttgttc atcattgcta ttaataagtt gtttgcgtag
    10921 gttagctgaa attcctttaa gccttgggag agttcgcatt atggcaagat caaatgtcgc
    10981 aatatctcgt ccttcaaatg cagcaacgtt gccttcatcc agcatttgca attcctgcac
    11041 taactcagga gaaggcttag ggcgaaacag cgcttctttt ttcatctgat tacagacatt
    11101 ccctgccatg cgaatattgc ttcgcttaaa aatttctttc agagcatctt catcatttat
    11161 tagaccccaa taatatgcat caggatttat tatcatcagc gcttttttga cattttcagt
    11221 gtcatttttg tcactcgcac ggaccatgaa ttcactattt gtcgaagacg caacaacagg
    11281 tgcaattatg tcattgataa atattccacg cgcattaata ctccccttaa tcgaagacgg
    11341 attatcgtaa tatgagtcga taaataccag tccacttgca tttatcgaac cggtaattaa
    11401 cagtggtgaa tccgtaacta tattcgttgc cgtcacgtta ccgagaataa cccatggatt
    11461 agaaagagag aaagtggaaa tattaccttt gactgtcaaa ttaccaagaa caatcaattt
    11521 ttgattactc tctatattac cttctaccac taaatcgcca cgatgcactt tccagtccga
    11581 tgcagataaa ccgaagggga caagattcac gctactgttg atagcttgtt caacttgttg
    11641 tttatcttca gcggtagccg cagaaaaaaa tggttcaata tcaactgtat ttgcaacaca
    11701 tcgaagttcc gcctgtactg gaagactcag cataaacagg ggaagtaaac ctatccagcc
    11761 gcaaatcctg gacttaaaca tatgcactcc atgccattaa aaaagaaaca aaagggaaat
    11821 gcatctttcc cttttgtata ttcgttattc tcgcgaaata ccatccaacg tagacaacac
    11881 ttcctcagaa agatgcaatt tttcagcagc catattctcc cgtaaatgcg caacagaaga
    11941 cgtccctggg atcagcaaaa tattcggtga acgttgtaac agccacgcca gcgccacctg
    12001 catgggtgtt gcacccaggc tcgcagcaac atccgacagc gtagaggatt gcagcggtgt
    12061 aaagcccccg agcgggaaga acggcacgta ggcaatgcca tcgtgggcca aagcatcaat
    12121 cattgcatca tcagcacggt gcgcgatgtt gtattcgttt tgcacacaga caatttcggc
    12181 aatcttgcgc gcctctgcaa cctgcgtcgg tgtgacgttg ctcaggccaa tatgttttac
    12241 caggccttgt tgttgcatct ctgccagcac ggtcaggctg gcctcaatcg atccttccgc
    12301 tgggccatga ccatctccca tcataacgcg caggttaacc acatccagta cgtccaggcc
    12361 gagattacgt agattatcgt gcaccgcttt ttgcaactct gccggagaaa aggcgggcaa
    12421 ccaggaagcg tcctctccac gccgcgcacc aattttagtg acaattgtca ggtcgtcaga
    12481 gtaaggataa agcgcttcgc ggataatctg attggtgacg tgcggaccat aaaagtcgct
    12541 ggtatcaatg tgattgacgc ccaatgccag cgcctcgcgc agcacagtta tagcgacgtg
    12601 gcgatctctt gggggaccaa aaaccccagg acctgccagt tgcatcgcgc cataaccaag
    12661 acggttaacg gattttgtac cgagagtaaa tgtattgctg ctcatcgcac cctgccctgt
    12721 aataaaacgg attaagaaaa atcagtatag aaacgaaaag agataaataa tgcgagggcg
    12781 taaacaagat tactggatga gttgctctat tgtcctgaaa aacacaaaac aaaacgcgta
    12841 aagcgccgca tccggcaata aaaaaaccgc aacctgtatc agcgttgcgg tttaggtatc
    12901 acatgctaaa aattagaatg ttcagtatct ggacgttact gtttcggtcg cagattctca
    12961 tcaaatacca gacgtttcct gttcggttca acttcccgca atggctcaac ttgatgcaac
    13021 gtctgcttac aacgagtcac cagcacctga tactcatgcg taccctgctt tttccatgcc
    13081 agctcctgct cactgagttc acgaatcgct ttcgccgggc tgccgacaat caggtaattg
    13141 gcaggcattt ctgctttggc tttcacaaat gcggatgcgc caacaatgct gttctcgcca
    13201 atcaccgcgc cgtccatcac taccgcgttc attcccacta atgcattgcg gcggataatg
    13261 caaccatgaa ggatagcgct atgaccaata tgtccatctt cttccacgac agtatcctgc
    13321 tcgggaaaac cgtgcataac gcaattatcc tgaatgttcg cgccatcttt caccacgata
    13381 cgaccaaaat cgccacgcag gctggcattt ggcccaacgt aaacgccctt gcctagaata
    13441 acatcgccga tcaataccgc tgtcggatgg acaaaactct cttctggcac aaccggagtc
    13501 agaccatcta tctggtaaat tggcatatcg cctcctgttc aatattcaaa ccgccaaaac
    13561 gttgaaaata caggcttccc ggcgcaggca gttcaccgac cgaggtttca cctttttcac
    13621 taacgaacgc taaagcagca ggcgctacgc gctgataaat gttgatacac agctgacgcg
    13681 ccgtatgccc tgcccagtgc gccggaagca actcctctgg caacaatggg tctttaagga
    13741 cgacacggcg ataaaaatgg atcagtaaaa gctgaatatg aaatgcacgc tccggggtta
    13801 actcgtctgc tgcctctttt aacagcggca ccagcgggcg gaatgactga ataaaggttt
    13861 cgtacatggc attttgttca gttaaatgcc agcactcttc cactctggca cgcagtgctg
    13921 cacgagaaag cgccagtggt atttgcgctt caaaacaaat cacgttatcc gccacccccg
    13981 cttcatgcaa aagtgtctgt acatccgcca gtttttgcga cggcgatgcc atcaggctgg
    14041 gtgccagtgc gccaaaacct tgccagatca actgcttttt gacatcagcc agcgttgatt
    14101 tatctaaacc ttccgagagc aacaggagcc atttaccatc ccatgcaggt tgctctgcgc
    14161 gataaatttt actttctgcc cgtcgcgtca ggcgcaagcc tttatcactg aggctataga
    14221 aactgcgtcg cccgatgcgg gaaacatcca gccagccttc tttattaaga cgaaacaaag
    14281 cggtgcgcac gaaacgctca ccaaatccca gcccttccag caaagcagcc agactaccca
    14341 gccagatttc accaccacga tgggaaagcg aatcaccata cagagaggag atcaaagatg
    14401 tgccactgac cggaacagcg tttacagcat gttggataaa agtaacaagt ttactcattc
    14461 gattcagtct gttgttattg ttgtgattta tgaatcatag cacaggtcgt atcgacttct
    14521 ggccctggcg ctttacctca ccagggccag acgacacgtc aggcaccaac aatattgcgc
    14581 agatcaaaca cccgacacgc cttgccttct gaacgcggga tactgccaca gttaacgatc
    14641 atcacatcgg tagagatccc caccatcgac ttaatccgat ggcgcagctg gtggcacacc
    14701 tggcaacgct gctcatgcgt caatgtcagg ctgctttctt tcaactccac tttcacagaa
    14761 agtgaatcaa gatgcccccg gcggttcacc tccagttggt aatggggcga taaatgttcg
    14821 aatttgacaa tctcttcttc cagttgtgac gggaagacat ttacaccgcg aatgatcaac
    14881 atgtcatcgc tgcgtccgct gatgcgatcc atgcggcgca tagtccgcgc cgttcctggt
    14941 aacagtcggg ttaaatcacg cgtgcggtaa cgaatgaccg gcaatgcttc tttggtcagc
    15001 gtggtgaata acagttcgcc atgttcacca tcggcaagcg gtgtgccgtc atgaggatta
    15061 acaatttcag gatagaaatg atcttcccaa atggttgggc cgtcggtagt ttccagacac
    15121 tccatcgcca cccccggccc catcacttct gacaggccat aaatatccag tgcggtgatc
    15181 cccagacgcc gttcaatctc tttacgcatg gcctgtgtcc acggctcagc accaaacact
    15241 ccaacccgca gcgagcaacc gctggcatca ccgcccaact gccgctccag ctcttcaatc
    15301 aggttaaggc aataagatgg cgtaaccatg atcatatctg gttgaaaatc gcggatcagt
    15361 tgcgcttgtt tttccgtctg cccaccagac atcgggatca ccgtagcgcc cagacgttcg
    15421 gcaccatagt gcgcgcccag cccaccagta aacaggccgt aaccataggc aacatgaatt
    15481 ttgtctttgg gcgtgccacc tgccgcacgc aaggaacgcg ctacaatatt ggcccagtta
    15541 tcaatatcgt tttgcgtata gccgacaact gtcggtttac ctgtggttcc cgaagaagca
    15601 tgaatgcgca ccacttgttc catcggcacg gcaaaggtgt cgaagggata gttgtcgcgc
    15661 agatcctgtt tggtggtaca gggaaattta cgcagatctg aaagttccct gaaatcatca
    15721 ggatgtacgc ctgctgcgtc aaatttgcgc cgatacatcg ggacattttc atacgcgtgt
    15781 ttgagcgtcc atttcagacg ttgtgtttgc aacgcctgta actcatcaac agacgcggtt
    15841 tcaatcgggt caagctttgt attggttatc attgtagggt actcgcaggt tgatatgctc
    15901 aaacacgctc cagaatcatg gcgatgccct gaccgacacc gatgcacatg gtgcacaatg
    15961 cgtaacgacc gttacgccga tgcagctcat ggctggcagc cagtgccagg cgggcaccac
    16021 tcattcccaa cggatggcct aaggcgatag cgcctccgtt ggggttaaca tgtggggcat
    16081 catcaggcag ccccaattcg cgtagtacac ccaacgcctg ggccgcgaac gcttcgttca
    16141 gttcaatcac gtccatatcg tgaatactca gccccgcgcg ttccagcacc cggcgagttg
    16201 caggcaccgg accaggcccc atcaggcgcg gttccacccc ggcggttgcc atggctacga
    16261 ttcgcgcccg cggtgtcagt ccttgcgctg ctgccatctg ttcactggca ataatcaacg
    16321 cagcggctcc gtcattcacc ccggaggcat tgcctgcggt aatcacccca ttggcacgaa
    16381 atggtgcttt taacccacgt aactgttcca gcgtcgtttc cgggcgcaga tgctcatcat
    16441 gttgtatttc tgttacaacg cctttcttgt ttttcaacac aaccggaaca atctcctcag
    16501 ccagaatgcc tgaggattgc gcttttgccg tacgttgctg actgcgtagc gcaaaactat
    16561 cttgatcttc tcgcgatatt tttaacagtt cagctacatt ctctgccgtt tccggcatgc
    16621 tgtcagttcc aaattgttga gccatgagcg ggttcacaaa tcgccagcca atagtggtat
    16681 caaacatctc agcctgacga gaaaatgcac tggttgcctt gcccataaca aacggtgccc
    16741 gagacattga ctccacgcca ccagcgatca gcaaatcgcc atcgcccgct ttaatcgccc
    16801 gtgcggcgaa ccccagtgcg tccagcccgg aaccacacaa gcggttaatg gttgtgccgg
    16861 aaacactatg cggcagcccc gccagtaacg tcgccatccg ggctacgtta cggttgtctt
    16921 ctcccgcctg attagcacag ccgaggatca catcatcgat acactccgca tcgagacgcg
    16981 ggtttcgcac cagcagctcc cgcaaaggga tagcagccag atcgtcagcc cgaacacctg
    17041 ataatgcccc accatagcgg ccaattggcg tacgaattcc gtcacagata aaggcttcac
    17101 gcatcaggct tctcctgtaa tggtgccgcc gatgcggtga gatttaccgc gaaacagcgc
    17161 aaccgttttt tgttgttggt taacaatttc gatgtcgtaa acaccggttt gcttgccctg
    17221 atgacgtacc tgcgcagtag cagttaaggt gtctccggca aagcctggac gcaaaaaatc
    17281 aatcgtgcag gcagaagcga cggctgccag cccctggcta ttgcaggcgt aggcaaaggc
    17341 agtatcagcc agtgaaaata gctgcccgcc gtggcaactt tgatgaccgt taagcatttg
    17401 tgcagtgacg gtcatggtca ctacagcaaa gccttcatcc attgagataa tgtcgatgcc
    17461 aagcgctttg gcgcaggcat cgttctcata cattgcatgg gcattttgcc aggccttatg
    17521 actcataacc gctctccaga agcgctcgtt ggcgcagcaa tgaacatggg cgatagcgtt
    17581 cttcgccata gtgatgttgt agattttcca ggagccttaa tattcgctgc cagccaagtt
    17641 gcgctcccca ggcaagcggg ccatatggat aattcacccc aagacgcatg gcggtatcga
    17701 tatcctgttc agaggccacg cctttttgca gcgcatcaag ggcttcattg atgatcattg
    17761 ctaccgttcg ccaaatcagc attcctgggt aatctgcaat ttgcagcact gttttgccct
    17821 gctgttgcag gtaataaatg gccttgcggg tcgctgagtc cgggttcacc gctgccgcag
    17881 caatggtcac caccttgccc gccattttgt cgaccactac caccgggcgt gccagtcgta
    17941 ttgccagcgc ctgtgccgtc tcgccttgtg tctcaatcaa taaaacatcg tcaatttccg
    18001 tgacgccgtc acttttcttt tctactttca ttgggctaaa actgtcgctt accgcttcca
    18061 ggccaacaac tgcctcacgt tccgcgcgcc agtcgtacac gcccagccca cttttcttgc
    18121 ccaaccgtcc accaatcacc agttcctgtt gcaccagcga aggtaaaaaa cgacgctcct
    18181 gccagaaagc gttaaacacc gaacaggtga cagcaaaatt gacgtcctga ccaatcagat
    18241 cggttaattc cagcggcccc atcgggaaac cagcgccatc gcgaagtgca gcgtcaatca
    18301 cttctggtgc agcaacctgc tcttccagtg cccgccaggc ctcggaataa taaggacgcg
    18361 ccacacggtt aacgataaat ccaggggtcg aatgacagcg cacaggctgc ttaccccaac
    18421 tcaacgttag ttcacacaac tgctcaacaa cttccgctgc cgttgccagc ccactgacca
    18481 cctccaccaa cttcatcacc ggtgccgggt taaaaaaatg cagccccgca acacgttcag
    18541 gattttttac ctccgcagca atcgcggtta tagagattga cgaagtgtta gtggtcaata
    18601 gcgtttgtgg cgggcaaact tccgccagct gtgcaaagag cgcttttttg acttccagac
    18661 gttcagacgc cgcttcaatg accaggtccg cagctgccag cgcgtgaata tcggtcaccg
    18721 ggatcaggcg tttcaatgtg cgttcacagg tttcagcagt cagttttccc cgcgtcacgc
    18781 gtgaatttag ccgcgcgtgt atcccgtcga ttgcgcgggt cagcgcttca gcagaaatgt
    18841 catacagtaa aacctgatgt ccatgactgg cggcaacttc agcaatgcca gcccccatgg
    18901 tgccgctccc aatcactgcc acagtttgca catttatcat catgctattt ccccgtgaac
    18961 tgcggtgagc gtttagccag gaacgcactg acaccttcac gataatctgc gctccgtccg
    19021 gcaagtcgct gatagtcgcg ttccagatcc agttgcgtat cgagcgtatt ggtttcagcg
    19081 ctatttatcg cttgcttgat aagtccaaga ccaaatgtcg gttgtgtcgc cagatgccgt
    19141 gccagctgtt gcgcggtatc tgccagcgtt tcatcatcaa caacctgcca gatcatcccc
    19201 cattcgtgcg cctgttcagc actcagttga ttccccagca gtgccagccc catggcgcgc
    19261 gctcgcccag caacgcgtgg cagtaaccag gttccaccgc aatcgggtat taagcctaac
    19321 ttactaaacg ccatgacgaa ttttgctgaa cgggcagcaa taacgatgtc gcaccccagt
    19381 gccagtgttg cgcctgcccc tgccgccacg ccattgactg cacagatcac cggttttggc
    19441 agttttgcca ggcgacgtac cagtgggtta tagaaacgtt caactgacat ccctaaatcc
    19501 ggtgcggggc ctgtgggatc aacgttacga tcgttaagat cctgaccagc acaaaatccg
    19561 cgcccggcac ctgtaagtaa caggcaacgg atagtgtcgt cgcgctcgac ctgtttcagg
    19621 cactctgcca gttgtgcgtg catctcatca ttaaaactgt tcaggcgttc cgggcggttg
    19681 agcgttagtg tcatcacgcc cttttctaca tgactgagga tgaattccat cattagcgtc
    19741 ctttaaagtc gggcgtgcgt ttttgtaaga aagcggagat gccttcatga cgatcttctg
    19801 ttgccgccag caaggtgaat aactgtcgct cctgggcaag tccggcttgc aaagccactt
    19861 cctgcgactg gcgcagcgct tgctttgccg cttgtaaggc caacggcgag tgacgtgcca
    19921 ttttcgatgc cagctgtaag gcgtattcga gggttaaatc gctggggaag acgtcgctaa
    19981 ccagcccggc ctgctgtgct tgctgggcgg tgatactttc tccgctcagc accattttgc
    20041 tggctaacga tttaccgaca ctacggatta aacgttgcgt tccgcctgcg ccaggcatga
    20101 tgccgagagt gatttccggc aacccaaaac gcgcgttctc tccggcaacc accacatcgc
    20161 acaacaatgc cagttcgcaa cccgccccaa gcgcgtaacc attgacggct gcgataagtg
    20221 gtttattaaa ggcctgcaat cgcgcccata gctgcggacg tgtatcgttt aaggtggccg
    20281 cgagatcttt ttctgccatt tcgttgagat cggccccagc ggcaaaaaag cgtgcattac
    20341 cggtaatcac acagaccgaa atgctggtat cggtagccgc agcttccagt tcatttacca
    20401 gttgcatcag cagggcatta tttagcgcat tacgtgcggc gggacggtta agggtcagca
    20461 acaatactcg ttgctgacgg ctgacgatca gttcgctcat gccatcccct tcgcgtcaaa
    20521 gtcaaccacc acatcgctgg tcagcggcag tgcctggcaa ctcaacacat aacctgcggc
    20581 cagttcatcc ggttccagac tgtaattggt ttccatcgcc actttgccac gcagcacttt
    20641 gcatttgcag gtcgcacaga cgccgccttt gcaggcatag ggcagatcag ccccctggcg
    20701 caatgccgca tcgagaatgc tttcatcgtc ggcattaagc acgatttccc gatcccgccc
    20761 atcctgacgt acagtcactt tttgtccgtc actttgcacg ttaacgctac gtttgacgcg
    20821 cgtgccaggc gtattaaacc gctccagatg aatggtttta tctggcattc ccagtgcttt
    20881 taaggcggct tccgtctcat ccatcatcgc cgccggacca caaataaatg cctcatcata
    20941 aagacgaaaa ttaatgagcg aggccccaag tgactgtaat ctttcaccgt caatacgccc
    21001 gtgaagcaga tcgctgtcga gggtttcctg actgaaaatg cacaacaact gtaaacgctg
    21061 aggatatttg tctttcaggt ctgccagtgc ctggcgaaac atcatgctct ggctggtacg
    21121 gttaccgtag atcagggtga actgactttc aggctcggtt tgtaaagtgg tggcgataat
    21181 cgccagcatt ggcgtaatac ctgatcctgc tgcaatagcc agatagcggc cctggcgttc
    21241 ggcctggggc tgatagccga aatgcccctg cggcaccatg acctccagcg tcataccctg
    21301 gcggatgtgt tcgcgggcat agcgggagaa acgtccgcct tcaatggctt tcaccgccac
    21361 actaatttcg ccaggcaggt agctgcggca aatggagtaa caacggcgta attcttcacc
    21421 atcaagactg gcttttaagg tcaaatgttg accggggcga aagcgatacg cctcctgcaa
    21481 aggctggggc accgcaaagg taatggtcac cgcatcacgg gtttccggct cgacttttgc
    21541 cactgttaag gaatgaaacg ttgtcatggc atcctcaaat acatttgaaa taatcgaaag
    21601 gttcgcggca actatcgcag cggtacaatg ctttgcaggc cgtggaacca aattcactga
    21661 taagtgtggt atggacgctg gcgcagcgcg ggcaacgtac ttctggcggc aaatgggcat
    21721 ggcaactgtg tccggcgggc gggctaatgc catactgtcg cagacgctca cgggcatcgg
    21781 gggtcatcca gtcggtggtc catgccgggt cgagttgcag cacaacctga acgggggtaa
    21841 acccgtgggt tgtcattgcc tcacgtatcg cgccaatcaa atgttccgtt gccggacaac
    21901 cggaataagt cggcgtaaag ccgatcaccc atccttctcc catctgtgtc acattacgca
    21961 ccatgcctaa atcggtaatg gtcagcaccg ggatctccgg gtccgggatc tggctgagta
    22021 gcgcccatat ctcatgaacc tgtggcggtg caatggtggc cagacgttgc atacccatct
    22081 cctctgttac cattgctgac cgggcaagac acgctggaga tactgcattt ctgccagcat
    22141 gggtccaagg tgttcggtat gcagtccttt tttaccgcca gtgcgatacg cctgctcttg
    22201 cggtacgttc aatgtggctt cgttgattcc ggcaaaaact tcggcttccc acgctgcgcg
    22261 taaagtgcgt ggatcaaccg caataccctc ttcactcagt gcaatatcaa tctcgtcggc
    22321 atcgaacagt tcggcagtaa aacgccacaa cttgtcgatg gcctgctgca tcttttgccc
    22381 tgatacgtca gtaccattgc ccagtcgctc cagccagcca cgactaaaac gcaggtgata
    22441 acgcgcttct ttaattgctt tggcagaaat cgccgccagt tgcggatcac ggctttccat
    22501 cagacgggta aagagcgcca catgccatgc atcgatgaaa tactggcgtg caatggtgtc
    22561 ggcaaaattg ccgtttggtt gttcaaccaa caataagttg ctgaactggc gctcgtctcg
    22621 ggtgaaggcc agggtatctt catcaccttc tcccgctaat tcagcggcat acgataagaa
    22681 gttacgtgcc tgacctaata aatcgaggcc aatgtttgcc agtgcgagat cgatttccag
    22741 ttccggtgcg tgaccgcacc attcccccaa ccgctgggag agcaccagat agttatcgcc
    22801 caggcgcaag gtgtaagccg ttaactgatt catttccgac ctcacatgtg ctcaatgcca
    22861 tcagggatgg tgtaaaacgt tggatggcga tagaccttgc tttcagccgg atcaaaaaat
    22921 tcaccgcgtt cttccggttg cgaggcaaca atttcactcg ccttcaccac ccaaattgaa
    22981 catccttcgc tacgacgggt gtaagcatca cgggcatttt ccagtgccat ccgctcatcg
    23041 gcagcatgta aactgccaac atggcggtgt gataagccct gtttgccacg cacgaaaact
    23101 tcgtataacg gccagtaaac attactcatt ttgaatctcc ttatgcgacc ttacgggcaa
    23161 gttgtttttg tgcatgggcc agcgctgctt cccgtaccca ggtaccttct tcccaggctt
    23221 tacgtttggc gtcgagcctc tcctgattac aaattccgcg accgttaatt acttcgttaa
    23281 actcctgcca gtcgatctca ccaaagcggt agtgaccgct ttcagtgtca aaatgcagat
    23341 ccgggtcagg aacggtcata ccgagcattt caacctgtgg aacggtgttg tccacgaaac
    23401 gctggcggag ttcgtcgttg gtgaaacgtt tgattttcca ggcgagactt ctggcgctgt
    23461 ttggcgagtt atcatcgttt ggcccgaaca tcattaaggc tggccaccag aaacggttaa
    23521 tggcgtcttg caacatctgc ttttgagctt cgctgccctg cgccagtgcc atgcaggctt
    23581 caaacccctg acgctggtga aaactctctt ctttgcagat tttcaccatt gctctggcat
    23641 acgggccata agaagttcgg cataacgcca cctgattgac gatcgctgcg ccatccacca
    23701 gccagccgat aacgccgata tcagcccaac tcaatgtcgg ataattaaag atggaggaat
    23761 atttcatccg tccgtcgagc attttttggt agatgtcttc tctggcacag cccagtgttt
    23821 cggcggcgct atagagatac agaccatgac cggcttcatc ctgcactttg gctaacagaa
    23881 tggctttacg ccgcaaggtt ggtgcgcgag tgatccagtt accttcaggc aacataccaa
    23941 caatctcgga gtgcgcatgc tgcccaatct ggcggatcaa tgtctttcgg taagcatcgg
    24001 gcatccagtc ctgcggctcg atagccgttt cctgggctat ccgttgctca aagcgttctt
    24061 cttgggtcac gtcatcacct ttacgattcc agttttcgtg aattaattaa aaacacgaat
    24121 cacgttgttt tatgaaagtt acacaatggt taactaaagt cacaaatttt gtatacttga
    24181 actgtgacag atttcgcact ctattttgat aactcattat tta
//
LOCUS       DAZFJF010000072        24028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFJF010000072 DAZFJF010000000
VERSION     DAZFJF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.072"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..3871)
                     /gene="entF"
                     /locus_tag="ACHKAU_003059"
     CDS             complement(<1..3871)
                     /gene="entF"
                     /locus_tag="ACHKAU_003059"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO5532076.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRAT"
     gene            complement(3868..4086)
                     /gene="ybdZ"
                     /locus_tag="ACHKAU_003060"
     CDS             complement(3868..4086)
                     /gene="ybdZ"
                     /locus_tag="ACHKAU_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO5532077.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4089..5290)
                     /gene="fes"
                     /locus_tag="ACHKAU_003061"
                     /pseudo
     CDS             complement(4089..5290)
                     /gene="fes"
                     /locus_tag="ACHKAU_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            5533..7773
                     /gene="fepA"
                     /locus_tag="ACHKAU_003062"
     CDS             5533..7773
                     /gene="fepA"
                     /locus_tag="ACHKAU_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO5532078.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7813..7911
                     /locus_tag="ACHKAU_003063"
                     /pseudo
     CDS             7813..7911
                     /locus_tag="ACHKAU_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7948..8568
                     /gene="entD"
                     /locus_tag="ACHKAU_003064"
     CDS             7948..8568
                     /gene="entD"
                     /locus_tag="ACHKAU_003064"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO5532079.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8690..8842)
                     /gene="hokE"
                     /locus_tag="ACHKAU_003065"
     CDS             complement(8690..8842)
                     /gene="hokE"
                     /locus_tag="ACHKAU_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO5532080.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9193..9345)
                     /gene="hokE"
                     /locus_tag="ACHKAU_003066"
     CDS             complement(9193..9345)
                     /gene="hokE"
                     /locus_tag="ACHKAU_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO5532081.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9314..9583
                     /locus_tag="ACHKAU_003067"
     CDS             9314..9583
                     /locus_tag="ACHKAU_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532082.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            9787..10905
                     /gene="ybdK"
                     /locus_tag="ACHKAU_003068"
     CDS             9787..10905
                     /gene="ybdK"
                     /locus_tag="ACHKAU_003068"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO5532083.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLKEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10971..11219
                     /gene="ybdJ"
                     /locus_tag="ACHKAU_003069"
     CDS             10971..11219
                     /gene="ybdJ"
                     /locus_tag="ACHKAU_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO5532084.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            11284..11652
                     /gene="ybdF"
                     /locus_tag="ACHKAU_003070"
     CDS             11284..11652
                     /gene="ybdF"
                     /locus_tag="ACHKAU_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5532085.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11746..12399
                     /gene="nfsB"
                     /locus_tag="ACHKAU_003071"
     CDS             11746..12399
                     /gene="nfsB"
                     /locus_tag="ACHKAU_003071"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO5532086.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12507..13754
                     /gene="ybdG"
                     /locus_tag="ACHKAU_003072"
     CDS             12507..13754
                     /gene="ybdG"
                     /locus_tag="ACHKAU_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO5532087.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13822..15198)
                     /gene="pheP"
                     /locus_tag="ACHKAU_003073"
     CDS             complement(13822..15198)
                     /gene="pheP"
                     /locus_tag="ACHKAU_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO5532088.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15300..18443)
                     /gene="cusA"
                     /locus_tag="ACHKAU_003074"
     CDS             complement(15300..18443)
                     /gene="cusA"
                     /locus_tag="ACHKAU_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO5532089.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVLIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18455..19678)
                     /gene="cusB"
                     /locus_tag="ACHKAU_003075"
     CDS             complement(18455..19678)
                     /gene="cusB"
                     /locus_tag="ACHKAU_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO5532090.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19694..20026)
                     /gene="cusF"
                     /locus_tag="ACHKAU_003076"
     CDS             complement(19694..20026)
                     /gene="cusF"
                     /locus_tag="ACHKAU_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO5532091.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20184..21557)
                     /gene="cusC"
                     /locus_tag="ACHKAU_003077"
     CDS             complement(20184..21557)
                     /gene="cusC"
                     /locus_tag="ACHKAU_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO5532092.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            21714..22397
                     /gene="cusR"
                     /locus_tag="ACHKAU_003078"
     CDS             21714..22397
                     /gene="cusR"
                     /locus_tag="ACHKAU_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO5532093.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22387..23835
                     /gene="cusS"
                     /locus_tag="ACHKAU_003079"
     CDS             22387..23835
                     /gene="cusS"
                     /locus_tag="ACHKAU_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO5532094.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5794 a         6543 c         6114 g         5577 t
ORIGIN      
        1 gcgttgcgcg aataatcggc ccaattttta caaacgcccc tggagagata atatccacat
       61 gcgcacaatc ctgacgatag atatccagct ccgcaatcca cggcgaccag gcacgttcgg
      121 gactcatacc ttcctgaagt gtgcgctcag caacaaataa cgttgctttt ccatcaaacg
      181 gaacgctatg ggcggtggtt aacaagcgca cagcatcagc gtagttgcct tcaatggtgg
      241 taaacaactc cgttgaagta cttccctgct gtgccgccag gaaggcctcg cgttcgcggt
      301 taatctccgc cagcacttcc gggtccagac cattagcctc tttttcctgc cagttttgcg
      361 tctctggcgg ccaggtatcc agcaaaccaa gaaatgccac ctgttcgcca cgggcacgca
      421 gtcgcgccgc aataccctgc gccagcgtac cgccaaggga ataccccagc aggtaataag
      481 ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc ttcgcagact tcatccaggt
      541 ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg aatgccaata atcgaccatt
      601 gtggatcgag ataacgcgag agcacgctaa actgccaggc aaaaccggac gccggatgga
      661 aacaaaacag cgtcgggcca ttaccttcac gcaacggcag aatggtttcg aatcccatgc
      721 gccgggagct gtcctcttca ccatcaataa tcgttgccag tttggcgacg gttgacgcga
      781 ccatcacctg ccccggcgtc acctgacgag caaactgtcg acttaactgc gctgctagtt
      841 tcattgccag tagcgaatga ccgcccagcg caaagaaatc agcatcggca tcctgcacgt
      901 cacaacccag caacgacgcg aatgccgcgg cgataatcgt ttcactgccc gctttcggtg
      961 cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa ggctttgcga tccagcttgc
     1021 cgttggcgct aagcggcaac tgcggaagtt gcagcagaac gaccggcacc atatgcggcg
     1081 gcaatgtttc gcgaagctgt gcctgtaatg cgctggtatc caacggcagt cccgattgcg
     1141 acaccagata gcccaccaac tgacgcgcat caccaccggt ggctgccgcc tggttaatca
     1201 cacaggcgtg ggtaactgcc tgttcgacat ccggcagcgc ctgcatcacg cgatcgattt
     1261 cgcccagttc gatacgctgc ccgcgaattt ttagctgatc gtcactgcgc ccgaggtact
     1321 ccaccgcgcc gttatccagc cagcgggcga cgtctccggt acgatacatc cgttcacctg
     1381 gggcaaaagg atcggcaata aaacggctgg cggtcagatc gggtcgtcca agatacccct
     1441 gcgccagttg gatgccggtg agatagagat cacccgccac acccggcggc accggatgca
     1501 tcatcgcatc aagaatacgc agacccgtat tccataccgg ataaccaatc ggcacactgc
     1561 tgccgcgcac ctgtgccagt tcctcgccaa aagccggata ccagctgaca tctaccgccg
     1621 cttccgtcgg gccatacagg ttatgcaacg gcgcgccggt taactgttgc cattcgcggc
     1681 ataaatcagc cggtaaggcc tcaccactac agaaaacctg tttcaacgtc gcgcaattct
     1741 ggcgagcggt ttgcggcgtc agcgatgcaa caaatgccgc cagcatcgac ggcacaaagt
     1801 gcgtggtcgt tacgccatat tcggcaaaga attgctgcat agcgagcggg tcacggtgcg
     1861 cttccggttc agccatcacc agttttgccc ctgcgataaa cggccagaaa aactcccaca
     1921 ccgagacatc aaaactgcac ggcgtttttt gggcaacgac atcttcgcct gtaagcgggt
     1981 agtgattttg catccataac aggcggttaa cgatagccgt ctgcccgacc attacccctt
     2041 tcggcctgcc ggtggagcca gaggtaaaga tgatataagc cgtgtggtgc ggttgtgaaa
     2101 gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt atagcaaagg cttgttaaat
     2161 tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt aattaacagc gacggacgcg
     2221 catcttccag catcattttc aggcgatcgt ccggatagcc ggtatccagc ggtagccagg
     2281 ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa aaagaccgag cgcggtagtg
     2341 ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg cagcagattc gccagcgcca
     2401 ccacctgctc gcgcatttcc cgatagctga acaggtaacg cgcatctgcc agcgccggag
     2461 catccggtgt ttttgccgct tgttctgcca ccagcgcgct cagcgtggtt tctggaatct
     2521 caacctgagt ggcgttgatc tgcgccagct gcgcatactc acctggcagc ataatatcga
     2581 catcgccgca caacagcgcc ggatccgcgg cgaactgggc aatcagcatt ttcaggcgtt
     2641 cagcatgctg gattaacgtt ggctcatcgt aacgctgttt attggcgagg atctcaatac
     2701 tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag gtcattaacc ggaccggttg
     2761 ccagggtatg ggtttgcgtc tgaacaccag gaatatccag ttggtaatca aataccttga
     2821 tattgagtac cggaccgaac agcggttcct ctcctgccgc tcgcccgctg tcacggacaa
     2881 tttgttcggc atcgtaacgt tgatgacgac gcattttttt cagttgtgct gccagtcggg
     2941 ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat acccaacggt aaaacgttga
     3001 gcacgggtcc gctagccgtc agcgccgccg agcccagtcg acgcataaag ataaatccgg
     3061 cggcatagtc catgcgattg cacaatcgcc ccagccacaa ggctgccagc gcaagggcta
     3121 aatcggtacg ctgcacacct gaaagttgcg tagccagctg gcggaattcc ccgtcggtaa
     3181 attccagttt caggcgcaga atatctgccg aagcgctgcg ccccgctaaa ggtgccggag
     3241 aaagtgacgc gggcggcggc agttgacgac gctgttctgc ccagaatgcc gcatcacgct
     3301 gccaggcttc gctttcgcgg tattgctggt actcttccac tacatcagcg aaaggcgtaa
     3361 acggcgaagc aggcgttggt tcgccacgca gccatgtgca gtaaatattg gcgatctggc
     3421 gagtaatggc cgggaaactg aagccatcga ccagcaaatg gtgataacgc tgataccagt
     3481 accagcggtt atccgcaaca tgtatcagct gatgaaagac cagtggttta ccgctatcga
     3541 cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg cgcagtaccg tgcggatcaa
     3601 tattggttcg caggtcgata atttctggca gttcgaatat cagcgcatca tcgacccact
     3661 gccagacttc gccgttatct tccgtaaaac gcatccgcaa cgtatctgct tgcgctagtc
     3721 cgacaaccac cgcgcgggcc agtaatggcg catcaacatc tccagttaac tcaacgtaat
     3781 gcgccacgct ccaggcggag ggtaaatccg acagtttttc tgccatccag atgccgggct
     3841 gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc tgcaactggg tgaaattggc
     3901 cggtgtcaga gtacgccagt ggacttccag ccactgctgg caggacgcct gtgactgcgg
     3961 ctgacacaca atgtcccagc ctgccggtaa ggcgcattgt tgcggccaca gactgaattg
     4021 cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgtgga tcatcgaagg gattactgaa
     4081 tgccatattc aactcctgtc atggaaagtg gttgccagag gtcgattagc ccctgcatca
     4141 agccaccgcg ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag aaaatggatt
     4201 cttttatggg gtgtaattgc gcatacagcg cctgattggc ccgcataatc atcggctcgc
     4261 gaatacccgc ttccagcaca atgcgcagac cttcggcgct aacttcacca gctttaagct
     4321 tttcaagtaa cacgccctct tgctgcccgc cccgatgcgg ccaccagtac gatcctgact
     4381 ggcttaatac acagccaaag cgttcaggcc agtgcagtcc ggcatacagc gcggacagcc
     4441 caccaaaact ctgcccggcg accacggtgc gatcggcgcg atcgctaaaa ggggcaatag
     4501 ctttcaccag gggtaataac tcttgctgta ctgccagcca gaaatccgca ttacacggca
     4561 gttcgtgggc gcggtgcgtg gtgtcgatag cgtcgatcaa cacatacacg gcgggaggaa
     4621 gttgctgacg atgggtcagc gaagtcagca caggccagac gggcatactt tgcgcccaaa
     4681 attcgccatc gagcaaaact gccagcggac gttcttctgc tgttgcatcg ccggtggtaa
     4741 aaatccatac acgacgtgaa tttttcaacc gttcactttt ccagataatt tctttggctg
     4801 ctatttctgg cgcttgcgga caatcccatc ccggttgcag aggcgcttgc ggcatttcga
     4861 gtgcagaaac agcgtgccct cgcccgcctt tccagctttg tggattcagc ggatcggcta
     4921 ttgcctgggg taatagtttt cgccagcctt cacgtaattc gaggcgatcg ggggatggtg
     4981 cagaaaaaat gtcatcgcgt tcggtgggaa taaagcagta gctgccgcgc cagttggcat
     5041 tgagttgtgt cgtccactgc cagacatcag tacctgcaat tcgctgcatc gactggggct
     5101 ggctgttctg atgatgatcg gtcacaccag tgatgtagat ccatacgcgc tttatcgtcg
     5161 agtattcttc agaaccttgg ggatcacgcc accagaaagt gacctcaaac atttcgtcat
     5221 tcagacgctg ccattccggg ccatgtttcg actgccacca gctctcactt cctactttta
     5281 acgccgtcac accataaccc catgtttact gtgcaatttt tcattgattg cagaaatata
     5341 ttgataatat tattgataac tatttgcatt tgcaatagcg taatggcgcg ccgtgggaag
     5401 cgcggacatt tattaaccaa ctgcactgcg tgtctttcag gatcaaaggt tttcgcggta
     5461 gcgggatgcg tcgtgttgag gacgaccatg cccgacagtt gcaattcgtg gcaaaaatgc
     5521 aggaataaaa caatgaacaa gaagattcat tccctggcct tgttggtcaa tctggggatt
     5581 tatggggtag cgcaggcaca agagccgacc gatactcctg tttcacatga cgataccatt
     5641 gtcgttaccg ccgccgagca gaacttacag gcgcctggcg tttcgaccat caccgcagat
     5701 gaaatccgca aaaaccccgt tgcccgcgat gtgtcggaga tcatccgtac catgccaggc
     5761 gttaacctga ccggtaactc caccagtggt cagcgtggga ataaccgaca gattgatatt
     5821 cgcggtatgg gtccggaaaa cacgctgatt ttgattgacg gcaagccggt aagcagccgt
     5881 aactcggtgc gtcagggctg gcgtggcgag cgcgataccc gtggtgatac ctcctgggtg
     5941 ccacctgaaa tgattgaacg tattgaagtt ctgcgtggtc cggcagctgc gcgttatggc
     6001 aacggcgcgg cgggcggcgt ggttaacatc attaccaaaa aaggcagcgg tgagtggcac
     6061 ggctcctggg acgcttattt caatgcacca gaacataaag aggaaggagc cactaaacgc
     6121 accaacttca gcctgaccgg tccgctgggc gacgaattca gcttccgctt gtatggcaac
     6181 ctcgacaaaa cccaggctga cgcgtgggat atcaaccagg gccatcagtc cgcgcgtgcc
     6241 ggaacgtatg ccacgacgtt accagccggg cgcgaagggg tgatcaataa agatattaac
     6301 ggcgtggtgc gctgggactt cgcaccattg caatcgctgg aactggaagc gggttacagc
     6361 cgccagggta acctgtatgc gggcgatacc cagaacacca actctgacgc ttacactcgc
     6421 tcgaaatatg gcgatgaaac caaccgcctg tatcgccaga actacgcgct gacctggaac
     6481 ggtggctggg ataacggcgt gaccaccagc aactgggtgc agtatgaaca cacccgtaac
     6541 tcgcgtattc cggaaggtct ggcgggcggt accgaaggga aatttaacga aaaagcgaca
     6601 caggatttcg tcgatatcga tctcgatgac gtgatgctgc acagcgaagt taacctgccg
     6661 attgatatcc tcgttaacca gacgctgacg ctgggtacgg agtggaatca gcaacggatg
     6721 aaggacttga gttccaatac ccaggcgctg accggggcga acaccggcgg cgctattgat
     6781 ggtatgagtg ccaccgaccg tagcccgtat tcaaaagcag aaattttctc gctgtttgcc
     6841 gaaaacaaca tggagctgac tgacagcacc atcgtaacgc cggggctgcg tttcgatcat
     6901 cacagtattg tcggcaataa ctggagcccg gcgctgaaca tatcgcaagg tttaggcgat
     6961 gacttcacgc tcaaaatggg cattgcccgt gcttataaag caccgagcct gtaccagact
     7021 aacccgaact acattctcta cagtaaaggt cagggctgct atgccagcgc gggcggctgc
     7081 tatctgcaag gtaacgatga cctgaaagca gaaaccagca tcaacaaaga gattggtctg
     7141 gagttcaaac gcgacggctg gctggcgggc gtcacctggt tccgtaacga ttatcgcaat
     7201 aagattgaag cgggctatgt ggctgtaggg caaaacgcag tcggcaccga tctctatcag
     7261 tgggataacg tgccgaaagc agtggttgaa ggtctggaag gatcgttaaa cgtaccagtt
     7321 agcgaaacgg tgatgtggac caataacatc acttatatgc tgaagagtga aaacaaaacc
     7381 acgggcgacc gtttgtcgat catcccggag tacacgttga actcaacgct gagctggcag
     7441 gcgcgggaag atttgtcgat gcaaacgacc ttcacctggt acggtaagca gcagccgaaa
     7501 aaatacaact acaaaggtca gccagcggtt ggcccggaaa ccaaagaaat cagtccttac
     7561 agcattgtgg gtctgagcgc gacctgggat gtgacgaaga atgtcagtct gaccggcggc
     7621 gtggacaacc tgttcgacaa acgtttgtgg cgtgcgggta atgcccagac cacgggcgat
     7681 ctggcagggg ccaactatat cgcgggtgcc ggggcgtata cctataacga gccgggacgt
     7741 acgtggtaca tgagcgtaaa cactcacttc tgatgctaac gtcagattgt tgacaaagtg
     7801 cgcgtcgttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa atcttgccaa
     7861 ttcaatatat tgcaggacca tgtaggcctg ataagcgtag cgcatcaggc agtttagcgt
     7921 ttgtcatcag tctctaatat ggtcgacatg aaaactacgc atacctccct cccctttgcc
     7981 ggacatacgc tgcattttgt tgagttcgat ccggcgaatt tttgtgagca ggatttactc
     8041 tggctgccgc attacgcgca actgcaacac gctggacgga agcgcaaagc cgaacattta
     8101 gccgggcgga tcgctgcaat ttatgcgctg cgggaatatg gctataaatg tgtgcccgca
     8161 atcggcgagc tacgccaacc ggtgtggcct gcggaggtat acggcagtat tagccactgc
     8221 ggggctacgg cattggccgt ggtttcccgt caaccgattg gcgttgatat cgaagaaatt
     8281 ttttctgcgc aaacagcgac cgaattgaca gacaacatta ttacaccagc agaacacgag
     8341 cgactcgcag actgcggttt agccttttct ctggcgctga cactggcatt ttccgccaaa
     8401 gagagcgcat ttaaggcaag tgagatccaa actgatgcag gttttctgga ctatcagata
     8461 attagctgga ataaacagca ggtcatcatt catcgtgaga atgagatgtt tgctgtgcac
     8521 tggcagataa aagaaaaaat agtcataact ctgtgccaac acgattaatt gacaacatct
     8581 ggtacgattc gcccgcagcc atcactgacc gcgggcgaaa gtgtaaagca ggtgccttac
     8641 cactctgacc tgacaaccgg atatgcgggg attgctcccc gcacaacggc tacttcttcg
     8701 gttcgtaagc gagaacagcc ttaaactcaa tattacgttc ctttacggtg aactcacaca
     8761 gcgagtctcc gaccagaagc gtaaatccca gtaccgtcaa acacagcact attactgcca
     8821 caagggcata tttcgtcagc atgtttatat tgcctccatg cgaagaaggg actaccatcc
     8881 gagttgttga ggctcggagt ggcagcccca ggattgatag aaatatctcc tggggcttca
     8941 tctttccaga cctcagagtt aacctgaaac cggaaagctt caggcacccg ccgttatctt
     9001 acccttcctc aacaaaatat ctgtttattt atacagtgaa tcctgtttcg gacttctgtc
     9061 ggcaaaactc atcattgctc ttaaaacgaa atcgcccgca gccatcactg accgcgggcg
     9121 aaagtgtaaa gcaggtgcct taccatcctg acctgacaac cggatatgcg gggattgctc
     9181 cccgcacaac ggctacttct tcggttcgta agcgagaaca gccttaaact caatattacg
     9241 ttcctttacg gtgaactcac acagcgaatc cccgaccaga agcgtaaatc ccagcaccgt
     9301 taaacacaga actatgactg ccgcaagggc atatttcgtc agcatgttta tattgcctcc
     9361 atgcgaagaa gggactacca tccaaattgt tcaggtttgg gtaggtagcc ccagggttga
     9421 tagaaatatc gtcctgggac ttcatctttc cagacctcag agtaaacctg aaaccggaaa
     9481 gcttcaggca cccgcagtta tcttaccctt gcttagcaaa atagctgttt atttatacag
     9541 taagtcctgt ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt
     9601 tctgcatgcc tcaatcgtca aaattcttcg ccaccagcca gccgtgccat aacttgtcac
     9661 attgcaaaag tagccaaatt gcttcagtca ccatcaccaa aaaaagaaac attccagaac
     9721 ttccgcatct cgcccagccg ctccgacttc tatactgaat agaaaacgcc aacttaagag
     9781 aaacctatgc cattacccga ttttcatgtt tctgaacctt ttaccctcgg tattgaactg
     9841 gaaatgcagg tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt
     9901 gacgcggtta aaaatgagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg
     9961 ctggagctgg cgacggatgt ttgccgtgat atcaaccagg ctgccgggca gttttcagcg
    10021 atgcagaaag tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc
    10081 acgcacccgt ttcagaaatg gcagcgtcag gaggtatgcg acaacgaacg ctatcaacga
    10141 acgctggaaa actttggcta tctcatccag caggcgaccg tttttggtca gcatgtccat
    10201 gttggctgcg ccagtggcga tgacgccatt tatttgctgc acggcctgtc acggtttgtg
    10261 ccgcacttca tcgccctttc cgccgcgtcg ccgtatatgc agggaacgga tacgcgtttt
    10321 gcctcctcac gaccgaatat tttttccgcc tttcccgata atggcccgat gccgtgggtc
    10381 agtaactggc aacaatttga agccctgttt cgctgcctga gttacaccac gatgatcgac
    10441 agcattaaag atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt
    10501 cgggtgatgg ataccccgtt aacccttagc cacgcggtaa atatggcggg attaattcag
    10561 gccaccgccc actggttact gacggaacgc ccgtttaaac atcaggagaa agattacctg
    10621 ctgtataaat tcaaccgttt ccaggcctgc cgctatgggc tggaaggcgt cattaccgat
    10681 ccgcacactg gcgatcgtcg accgctaaag gaagatacct tgcgattgct ggaaaaaatc
    10741 gccccttccg cacataaaat gggtgcatcg agcgcgattg aggccctgca tcgccaggtc
    10801 gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg tggctcgctg
    10861 attgggctgg tgaaaaagca ttgtgagatc tgggccggtg actaagtagg gaattgcctt
    10921 acagaagaat atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc
    10981 ctttagaaac cttgactacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc
    11041 tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc
    11101 tgatggatct tagccagctt tacactttat tgttttgtct gtggttttta gtgctgggcg
    11161 ctattgagta ttttgttctg cgctttatct ggcaacgctg gttctcgctg gcggattaaa
    11221 cgtcgattat cgccgtactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg
    11281 tttatggata agcaatcact gcacgaaacg gcgaaacgcc tagcccttga gttacccttt
    11341 gtcgagcttt gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaaaatt
    11401 tttatgctgt cgtcggagct gcgcggcgtc ccctttatca atctgaagtc cgatccacaa
    11461 aaatccctgt taaatcagca gatatacccg agcattaagc cagggtatca catgaataaa
    11521 aagcactgga tttcggtgta tcccggcgag gaaatctccg aagcgttact tcgcgatctg
    11581 atcaacgatt cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg
    11641 cgtccaggct aaagcggaaa tctatagcgc atttttctcg cttaccattt ctcgttgaac
    11701 cttgtaatct gctggcacgc aaaattactt tcacatggag tctttatgga tatcatttct
    11761 gtcgccttaa agcgtcattc cactaaggca tttgatgcca gtaaaaaact taccccggaa
    11821 caggccgagc agatcaaaac tctcctgcaa tacagcccat ccagcaccaa ctcccagccg
    11881 tggcatttta ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc
    11941 ggtaattatg tgttcaacga acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt
    12001 gcaaaaaccg cgatggacga tgcctggctg aagctggttg ttgaccagga agatgctgat
    12061 ggccgctttg ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgct
    12121 gatatgcacc gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc
    12181 aacgtcggta acttcctgct cggcgtggcg gcgctgggtc tggacgcggt acccatcgaa
    12241 ggttttgacg ccgccatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt
    12301 ctggtggttg ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa
    12361 tctcgtctgc cgcaaaacat caccttaacc gaagtgtaat tctctcttgc cgggcatctg
    12421 cccggctatt tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata
    12481 ggctgctgtt gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag
    12541 cgggtattga gatcgatcac accacctcga tggtgatgat tttcggtatt atttttctga
    12601 ccgccgtcgt ggtgcatatt attttgcatt gggtggtact gcggaccttc gaaaaacgtg
    12661 ccatcgccag ttcacggctt tggttgcaaa tcattaccca gaataaactc ttccaccgtt
    12721 tagcttttac cctgcagggg attatcgtca atattcaggc ggtattctgg ctgcaaaaag
    12781 gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc
    12841 tgctttcagt cttctcgttg ctggatgtta ttttgaatct ggcgcagaaa ttcccggcgg
    12901 catctcagtt accgctgaaa gggatatttc aggggattaa actgatcggc gcaatcctgg
    12961 tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc agcgattctg atcagcggtc
    13021 ttggtgcaat ggctgccgtg ctgatgttgg tttttaaaga tccgattctt ggtctggtgg
    13081 caggtattca gctttccgcg aacgatatgc tgaaactggg cgactggctg gagatgccga
    13141 aatacggcgc ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtgcgtaact
    13201 gggacaatac cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact
    13261 ggagcgggat gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca
    13321 ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg cacttgttaa
    13381 aaccttattt aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta
    13441 cggagtcgat attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga
    13501 acgaatatct gcgtaaccat ccgcggattc gtaaagatat gaccttaatg gtacgccaac
    13561 tggctccagg tgataacggt ttaccgctcg agatctacgc gtttactaac accgtggtgt
    13621 ggctggaata tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag
    13681 agtttggtct gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg
    13741 catttaagca gtaattaaaa aaaccgctct catcgaatgg atgagagcgg acatcgtttt
    13801 gtcggatgta gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa
    13861 tacaatccac accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag
    13921 cagcaaaatc atgccgagga aggcaatgca aagatagttg ccgaacggat agagcagcgc
    13981 cttaaactgt gtttcacgcc cctgacgtcg catcgctgca cgaaaacgca gatgcgccag
    14041 acagatcata atccagttca acagcagcgt tgctaccacc agcgccatca gcagaccaaa
    14101 cgctttttgc ggcagcagat agttgattaa taccaccagc gaagtgatcg ctccggaaag
    14161 catcagcgag ttaatcggca caccgcgacg gctgacgcga gtcaaaaact ttggcgcatt
    14221 accctgcaca gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac
    14281 tgacagcgat gctaccagaa tgacgaagtt cagcgcagaa gctaccacgt tgctgtcgag
    14341 attatggaaa atcatcacaa acgggctact gttggatttc acttccaccc acggatagag
    14401 cgccagtaaa accaccagtg aaccgatgta aaacagcagg atgcgataca ccacctgatt
    14461 taccgctttt ggaatgcttt tttccggatc gcgcgcttca gcggcagtaa tcccaatcag
    14521 ctccagaccg ccgaaggaga acataattac cgccagcgac aaaatcagcc cattccagcc
    14581 ggtggcaaag aaaccaccgt agcgccagag gttgtcgata ctggctttct cgccgccgtg
    14641 accagaaaac agcagccaca ggccaaagcc gatcataccg atgatcgcca gcactttaat
    14701 cagcgcaaac cagaactcgg tttcaccata taagcgcacg ttcaccaggt taacggcgtt
    14761 gatgataata aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg
    14821 catatagatg cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta
    14881 gttccagcca gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa
    14941 tgaaccggat accggctctt cgaccaccat ctcgccaagc tggcgcataa tcaggaaagc
    15001 gatgatcccg gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg
    15061 gccaatgcca agaaacagac cagtaccaat tgcgccaccc agcgcaatca gttgaatatg
    15121 acggttatgt aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac
    15181 ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta
    15241 acatttagtg cccatcgtcg caagacacaa tccacacggt taaacggggt atcctgcttt
    15301 tatttccgta cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac
    15361 agcgacagca aaggtgcggt gatcatgccg ccaatcatcg gtgcggcaat ccggctcatc
    15421 acctctgaac cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc
    15481 accgtcatcg ctttcgggcg cacgcgcagg accgcgccgt gatataacgc ctcatccagc
    15541 ttctgctcgc tgaatgtttg tggattattc aacgacggct cggcctctat ggcgtgacgt
    15601 aaatacatca gcatcaccac gccaaattcg gcggcgaccc cggcgagggc gataaagcca
    15661 gtgcccgtcg ccacggaaag atgaaagccc atccaccaca gcaaccagat gccgcccacc
    15721 agcgcaaacg gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaacgccaga
    15781 tacaacagca cgaagataat catcaacgtc atcggcacca tgagtttaag cttatggttg
    15841 gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc
    15901 actttttcag ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg
    15961 gcatcgatat aaatccagct cgtcgggcgc gcattctcgg ttttcagcat tgacggtccg
    16021 gtagagactt taatgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg
    16081 atcggcaact ggcgcagtgc ctgcggacta tcgcgccagc tttgcggata acgcagatta
    16141 attggataac gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa
    16201 gtcacaaaca actgcacatc cgccaccgtc ataccgtaac gtgcggcttt ttcacggtta
    16261 atctcaacgt tgatatagcg cccaccttcc agacgctcgg ctagagcaga agccacgcct
    16321 ggcaccgttc gcgctacttc ttcaatttgc tcagccatcg cgtcgatatc cgccagcaca
    16381 gtgccggaaa ctttaatgcc gatggggctt ttaatgccgg ttgagagcat atcgatacgg
    16441 ttacgaattg gcggcaccca cagattcgcc agccccggca gacgcacggt gttatccagt
    16501 tcctcaatga ttttgtccat tgtcatgcct ggccgccact gctcctgcgg cttaagctgg
    16561 atggtcgttt ctaccatctc cagcggagca gaatcggtgg cggtttccgc tttcccggtt
    16621 ttgccaaata cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc
    16681 atactcgccg cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaggtcg
    16741 ccttcattga tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc
    16801 agcaccgaaa gcgctgccac caacagcgtg gttttcggcc agtgcagtac tttcagcaac
    16861 agcggatgat aaacacgaat caaaaagcga ttgagcgggt tactgctttc cggcggaatt
    16921 ttgccacgga tccagtaacc catcaggata gggatcacta cgatcgccag cagcgccgca
    16981 cccgccatcg catacgtttt ggtgaacgcc agcgggccaa acagacgccc ttcctgccct
    17041 tccagggtga agatcgggat aaacgacaac gtgataatca gcagactgat aaatagcgcc
    17101 ggacccactt caacagatgc atcggtgatc acctgccagc gcgttttatt atccagcgtg
    17161 gcgtcaggat gctggtgctg ccactcttcc agccgtttat gtgcattttc gatcatgacg
    17221 atagcggcat cgaccatcgc cccgacggca atcgcaatgc cgcccaacga cataatattg
    17281 gcattcagtc cctggaagtg catgacaata aaagcaatac acaaccccag cggcaacgaa
    17341 ataatcgcca ccagcgccga gcgcacatgc cagagaaaca gcgcacagac taccgccacc
    17401 acaataaact cttccagcaa cttgccgctg aggttgtcga tggcgcggtc aatgagctgg
    17461 ctgcgatcgt atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc
    17521 agtttgtcct tcacggcggc gatcacttct cgggcgtttt tgccggatcg caggatcacc
    17581 accccgcccg ccacttcgcc ttcgccgttt agttcggcaa tgccccggcg catctccggg
    17641 ccaatcagga ccttcgcaac atcgcgcaga taaacgggca cgccattttc actggctttt
    17701 aaaacgatgt gattaaagtc gtcgagcgtt tgcagatagc cgctggcgcg caccatatat
    17761 tccgcttccg ccagttcgat cgacgaaccg cccgcttcct ggtttgaagc atccagcgcg
    17821 ctttttactt cggcgagact gatgccatac tgcgccaggc gctggggatc gataaccacc
    17881 tgatactctt tcaccacacc gcccaccgac gccacttccg caacgtcagg gatggttttc
    17941 agctcatatt tgagaaacca gtcctgcaat gagcgtaaat cggccagatc gtgcttaccg
    18001 ctgcgatcca ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggtcccagc
    18061 tcggcgctga ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc
    18121 gaacgcgccc agtacggatc ggtgccatct tcgaaaatga catacacata agagtcgcca
    18181 aactgtgaga aaccgcgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc
    18241 ggataagtca cctgattttc aacgatttgt ggtgcctgac cgggatagct ggttttaata
    18301 ataacctgca catcggagag atccggcagc gcatccactg gcgtattaat gatggtccag
    18361 gtgccccaga tgctcagaaa caacgcgccc atcagcacca aaaaacggtt cgccaccgag
    18421 cgacgaataa tccattcaat cattggttat tccctcaatg cgcatgggta gcactttcag
    18481 agcgcatccg ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc
    18541 tggaaaccac cttttcacct tccgccagac cagagcgtaa tgcggtgacg ccttgcgatg
    18601 cctggaaaac agcaacgcgt ttcggtacaa agcgcccgtc ggcatcaacg gtaatcaccc
    18661 gctgttcgct gccggtatca atcagcgctt gtgacggaat gagcagcatc ggttcgctgg
    18721 cggtgttgag ttgcaaccag gcgttcatgc ccggttttag cgcctcgtcg gcgttgtcga
    18781 cttccagacg cagctgcagc gtgcgggtcg cggcatccac gccaggtagc agcgtccatt
    18841 tgcggatggt gagtgtttta tccggtcgcg ccggaacggt gagggtaaac tgcgaggcat
    18901 ctttcaccag ccaggcgatg gactccggga tcgcagcagt gacccacacc gggtccatac
    18961 cctgaatttt cgcgaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg
    19021 cggtgatcac gccatcaatg ggcgctttga gcgtaaagcg agtctggatt ttttgcgtgg
    19081 cgatcaggcg gcgaatatcc gcctccggca ttcccgccag tcgcagccgc tcaagaatgc
    19141 cttcagtctg ggtcgccgta ccgccggttt cgcgcagcag taaatactca ctctgcgctt
    19201 ccacccagtc aggaatggtc aggtcgagaa gcggtgtgcc cttttgtact ttatcgccca
    19261 cggtaagcgg atacaccttg tcgataaacc cggcagcgcg agcctgcaca atggcatact
    19321 gatactcgtt gtaactgaca ttcgccggga aactctgggc aaaagtcagc ggcccgcgcg
    19381 tgacggtagc cgttttcacc cccagattct gcgtctgagt cgggtcaatg cgcacaccag
    19441 acgcagaact ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt
    19501 tacctggttt atcgaaccgc gtattggggt acatcgggtc gtaccagaat aagattttac
    19561 gttctgcggt cgacgttttt tctgcgggtg gttcagcctt tgcaaaccag gtaaaacctg
    19621 ccgcagaaat aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct
    19681 cattaaacct gggttactgg ctgactttaa tatcctgtaa taaagaaagg ttgccctgct
    19741 ggacaaaatt aaacgccact ttgtcgccag ttttaatttc actcattttc gtctgcggag
    19801 tgatggtaaa gcgcatggtc atctccggcc agttcacggc agcaatcgga tcgtgatgga
    19861 tggtgatttt tttgctttcc agatcaatac cctttaccac gccagtggcg ctaataacct
    19921 gcggttgtac ttcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa
    19981 taacggtaaa cagactgaac attgcgactt gcagtgcttt tttcatttat tctctcctgg
    20041 agttaaaagt catactctaa ataattcgag ttgcaggaag gcgacaagcg agtgaatccc
    20101 caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca catgcaactt
    20161 gaagtatgac gagtatattg ctgtcaaccg ccacctaagg cggtatacaa agaaatttcg
    20221 ttaacctgac gggcataatt caggtcgagt aaagtttgtc gggttgcaaa taaagaacgc
    20281 tcggcatcca gcacttccag ataacttact gcgccgtgct gatataatgc ccgcgcccgt
    20341 tgcaaagtaa tttgcagcga cgccagataa cgctgctggg cgctgatttg atcgttcagg
    20401 ctttgacgta atgccagcgc atctgccact tctttaaagg cgttctggat tttctgttca
    20461 taattcacta ccgactgctg ctggcgaatt tcggcgatat ccagattggc ctggttgcgt
    20521 ccggcattaa aaatggggat ctcaatttta ggaataaaat tccacatccc gctgctggcg
    20581 ttaaataatg acgacagatc gctgctggcg gttgatattc cgctggtcag ccttatagat
    20641 gggaaaaatg ccgcgcgtgc ggccccaata ttggcattag ccgccattaa cgcgtgttca
    20701 gcttccataa tatccgggcg ctgcaataag atttgcgacg acaatcccgc cggtaattta
    20761 acgctttgca ggctgtcgct gtttactgtc tgcgcttgcg gcagcttgcc gtagcttccc
    20821 aataacagtt gcaatgcatt attcgcctgc gccagttccc cctgacgttt agcgatgtcg
    20881 ctgcgggtac tttcaatcac cccgcgagcc tgttccagcg ccagaacatt actgctacct
    20941 gtcaacagtt gtttttcgac aaacgcatat gactgctgat aattacgcag cgtttcttcg
    21001 gctatttgca attgcgcata cgctagctgc tgattgaaat aactttgcgc gacattagaa
    21061 accagcaaaa tatgcaccgc gcgctgggct tcctcagtgg ctaaataatt ttggcgctcg
    21121 gcttcgctca tgttctttaa gcgaccgaaa aaatcgagat caaagctggc gttaaggccg
    21181 gtcgaaaact cccgtgtcgt ggcggtattc cctttaagat taccgctcca gctgccgctg
    21241 ccctcgccat tgagctgtgg gtagcggtcg gcatcggtca gacgatattg cgcccgcgtt
    21301 tcctgcactt tcagcgtcgc catgcgcaaa tcccggttat tcaccagcgc ctcgctaatc
    21361 agcgtcttta cctgattatc aacaaaaaag gtgcgccagc ccacgttctg gtagttatct
    21421 gctgcgttaa ccaagccgtt ctggctgagt gagaactgct gcggcacggg cattgccgga
    21481 cgctgataat ccggtgccag tgaacaaccg gttagcgcaa gggccacaca aaatggcaga
    21541 agtttacaag gagacatagg ctcataattt ctggtgattt tataccgcca actttactcg
    21601 ccaggctctg attttccggt gacaggaaaa tgacaaaatt gtcattttgc caataagcga
    21661 ttgccatctg atcccgctac tctagaattg cccgggcaac atgcggagga aaaatgaaac
    21721 tgttgattgt cgaagatgaa aagaaaaccg gagaatactt gaccaaaggg ttaaccgaag
    21781 ccggttttgt ggtcgatttg gccgacaacg ggctgaatgg ctaccatctg gcgatgaccg
    21841 gtgattatga tctgataatc ctcgatatta tgctgccgga cgtgaacggc tgggatatcg
    21901 tgcgcatgtt gcgttccgcc aataaaggga tgccgattct gttgcttacc gcgcttggca
    21961 ccattgaaca tcgcgtcaag gggctggagt tgggggcaga tgactacctg gtgaaaccat
    22021 tcgcttttgc tgaactgctg gcgcgggtgc gcactctcct gcggcgcggg gcggcggtga
    22081 ttatcgaaag tcagtttcag gttgccgatc tgatggtcga tctcgtcagc cgcaaagtca
    22141 cccgcagcgg cacgcgcatc actctgacca gtaaagagtt tactctgctg gagtttttcc
    22201 tccgccatca gggcgaagtg ctgccccgct cgcttatcgc ctcgcaggtg tgggacatga
    22261 atttcgacag cgacactaac gccattgatg tagcggtgaa gcggctgcgc ggcaaaatcg
    22321 acaacgactt tgagccaaag ctaattcaga ccgtgcgcgg cgtgggttac atgcttgagg
    22381 tgccggatgg tcagtaagcc atttcagcgc ccgttttcgc tggcaacccg cctgaccttt
    22441 tttatcagcc tggccaccat cgcagcgttt ttcgcctttg catggatcat gatccactca
    22501 gtaaaagtgc attttgccga gcaggatatt aatgatttaa aagaaattag cgccacgctt
    22561 gaacgggtac ttaatcatcc tgacgagacg caagcccgac gcttaatgac gctggaagat
    22621 atcgtcagtg gttattccaa cgtgttgatc tccctggcag atagccacgg caaaacggtg
    22681 tatcactccc ccggtgcgcc tgatatccgc gagtttgcac gtgatgccat acccgataaa
    22741 gacgcgcggg gtggcgaggt gtatctcctt tccggcccga cgattatgat gccaggccac
    22801 ggtcacgggc atatggaaca cagcaactgg cggatgatta acttgtcggt tggcccgttg
    22861 gtggacggca aaccgattta tacgctctac atcgcgcttt cgatcgattt tcatcttcat
    22921 tacataaatg atttgatgaa taaacttatt atgaccgcat cggtaatcag catcctgatc
    22981 gtctttatcg tactgttggc ggtacataaa ggtcacgcgc cgatccgcag cgtcagccgt
    23041 caaatccaga atatcacctc gaaagatctc gacgttcgcc ttgacccgca gaccgtgccc
    23101 attgagctgg aacagctggt actgtcgttc aaccatatga tcgagcgtat tgaggatgtc
    23161 tttacccgcc agtccaattt ctcagcggat atcgctcacg aaattcgcac gccgattacc
    23221 aatctcatca cgcaaacgga aatcgccctc agccagtctc gcagccagaa ggagctggaa
    23281 gatgtgctct actctaatct cgaagagctg acgcgaatgg cgaaaatggt cagcgatatg
    23341 ctgtttctcg ctcaggccga taacaaccag ctaatccccg aaaagaaaat gctcaacctg
    23401 gcggatgaag tcggcaaagt gttcgatttt ttcgaggcgt tagcggaaga tcgcggcgtg
    23461 gagttgcgat ttgttggcga cgaatgtcag gttgcgggcg atccgctgat gctgcgtcgg
    23521 gcgttaagca acctgctttc taatgccctg cgttatacgc cgcccggaga ggcaattgta
    23581 gtgcgctgcc agacggtcga tcaccaggtg caagttaccg tcgaaaaccc cggtacgccc
    23641 attgcgcccg agcacttacc gcgattgttt gaccgtttct accgcgttga cccttcccgc
    23701 cagcgaaaag gcgaaggtag cggcattggg ctggcgatag tgaaatcgat tgttgtcgcg
    23761 cataaaggca cggttgcagt aacgtcagat gcgcggggga caaggtttgt gattatgctg
    23821 ccggagagag agtgattatt aagttcaaat tcagggcgtg gtgcatcacc tcctggtatt
    23881 gttgatcaac aaattagaga aaaacttatt aaacatatga aatatcgaag gcttttattt
    23941 ttcaaagttt cacgagatgt ttgtatatga aacttagtta aatctgtgtt tcttccaatt
    24001 gtagatcaca atgccctttc taaatttt
//
LOCUS       DAZFJF010000073        23949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFJF010000073 DAZFJF010000000
VERSION     DAZFJF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.073"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..198
                     /gene="rrf"
                     /locus_tag="ACHKAU_003080"
     rRNA            83..198
                     /gene="rrf"
                     /locus_tag="ACHKAU_003080"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(313..756)
                     /locus_tag="ACHKAU_003081"
     CDS             complement(313..756)
                     /locus_tag="ACHKAU_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5532095.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            913..1842
                     /gene="metA"
                     /locus_tag="ACHKAU_003082"
     CDS             913..1842
                     /gene="metA"
                     /locus_tag="ACHKAU_003082"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO5532096.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2111..3712
                     /gene="aceB"
                     /locus_tag="ACHKAU_003083"
     CDS             2111..3712
                     /gene="aceB"
                     /locus_tag="ACHKAU_003083"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO5532097.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3742..5046
                     /gene="aceA"
                     /locus_tag="ACHKAU_003084"
     CDS             3742..5046
                     /gene="aceA"
                     /locus_tag="ACHKAU_003084"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO5532098.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5327..7063
                     /gene="aceK"
                     /locus_tag="ACHKAU_003085"
     CDS             5327..7063
                     /gene="aceK"
                     /locus_tag="ACHKAU_003085"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO5532099.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(7032..8606)
                     /locus_tag="ACHKAU_003086"
                     /pseudo
     CDS             complement(7032..8606)
                     /locus_tag="ACHKAU_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(8923..9747)
                     /gene="iclR"
                     /locus_tag="ACHKAU_003087"
     CDS             complement(8923..9747)
                     /gene="iclR"
                     /locus_tag="ACHKAU_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO5532100.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            9947..13630
                     /gene="metH"
                     /locus_tag="ACHKAU_003088"
     CDS             9947..13630
                     /gene="metH"
                     /locus_tag="ACHKAU_003088"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO5532101.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13850..15481
                     /locus_tag="ACHKAU_003089"
     CDS             13850..15481
                     /locus_tag="ACHKAU_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO5532102.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(15572..16261)
                     /gene="pepE"
                     /locus_tag="ACHKAU_003090"
     CDS             complement(15572..16261)
                     /gene="pepE"
                     /locus_tag="ACHKAU_003090"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO5532103.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(16644..17873)
                     /gene="sorE"
                     /locus_tag="ACHKAU_003091"
     CDS             complement(16644..17873)
                     /gene="sorE"
                     /locus_tag="ACHKAU_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO5532104.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(17925..18748)
                     /locus_tag="ACHKAU_003092"
                     /pseudo
     CDS             complement(17925..18748)
                     /locus_tag="ACHKAU_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(18759..19556)
                     /locus_tag="ACHKAU_003093"
     CDS             complement(18759..19556)
                     /locus_tag="ACHKAU_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO5532105.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(19622..20116)
                     /locus_tag="ACHKAU_003094"
     CDS             complement(19622..20116)
                     /locus_tag="ACHKAU_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5532106.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(20116..20523)
                     /locus_tag="ACHKAU_003095"
     CDS             complement(20116..20523)
                     /locus_tag="ACHKAU_003095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO5532107.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(20533..21339)
                     /locus_tag="ACHKAU_003096"
     CDS             complement(20533..21339)
                     /locus_tag="ACHKAU_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5532108.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(21409..22356)
                     /locus_tag="ACHKAU_003097"
     CDS             complement(21409..22356)
                     /locus_tag="ACHKAU_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5532109.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            22704..23576
                     /gene="rluF"
                     /locus_tag="ACHKAU_003098"
     CDS             22704..23576
                     /gene="rluF"
                     /locus_tag="ACHKAU_003098"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO5532110.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(23577..23849)
                     /locus_tag="ACHKAU_003099"
     CDS             complement(23577..23849)
                     /locus_tag="ACHKAU_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO5532111.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         5799 a         6117 c         6162 g         5871 t
ORIGIN      
        1 tgttttggcg gatgagagaa agattttcag cctgatacag attaaatcag aacgcagaag
       61 cggtctgata aaacagaatt tgcctggcgg ccgtagcgcg gtggtcccac ctgaccccat
      121 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
      181 agtagggaac tgccaggcat caaattaagc gaaaggccat cagtcacgat ggcctttttg
      241 cgttggtgaa aaaaatgccg ggtgcgacgc tggcgcgtct tctccagcct gggcaggcac
      301 ggcaggggcg gcttattccc ccacatacgc cagattcagc aacggatacg gtttccccaa
      361 atcgtccacc tcagagcgtc ccgtaacctt aaaacccacc ttcttataga acccaaccgc
      421 ctgctcattt tgctcattaa cgttggttgt cagttccggt gccatcgaga gcgcatgctc
      481 caccagcatc cgacccacgc cgcagccgcg cacatcagga tcgataaaca gcgcatccat
      541 atgctgccca cttaacaaca taaacccaac cggctgatcc cgttcattaa ccgcgaccca
      601 caacggtgct tccggcagaa aggaacgaac cagctcttcc agctcggccc gatactctgc
      661 tgataaaaaa tcgtgggtgg catcgacaga tcgacaccaa atcgcaacga gttcctcccc
      721 ttcctcatgc tgtgagcggc gaatactaat aaccattttc tctccttttc gtcattctta
      781 tattctaacg tagccttttc cttgaaactt tctcaccttc aacatgcagg ctcgacattg
      841 gcaaattttc tggttatctt cagctatctg gatgtctaaa cgtataagcg tatgtagtga
      901 ggtaatcagg ttatgcctat tcgtgtgccg gacgagctac ccgccgtcaa tttcttgcgt
      961 gaagaaaacg tctttgtgat gacaacttct cgtgcgtctg gtcaggaaat tcgtccactt
     1021 aaggttctaa tccttaacct gatgccgaag aagattgaaa ctgaaaatca gtttctgcgc
     1081 ctgctttcaa actcaccttt gcaggtcgat attcagctgt tgcgcatcga ttcccgtgaa
     1141 tcgcgcaaca cgcccgcaga gcatctgaac aacttctact gtaactttga agatattcag
     1201 gaacagaact ttgacggtct gattgtaacc ggtgcgccgc tgggcctggt ggagtttaat
     1261 gatgtcgctt actggccgca gatcaaacag gtgctggagt ggtcgaaaga tcacgtcacc
     1321 tcgacgctgt ttgtctgctg ggcggtacag gccgcgctca atatcctcta cggcattcct
     1381 aagcaaactc gcaccgacaa actctctggc gtttacgagc atcatattct ccatcctcat
     1441 gcgcttctga cgcgtggctt tgatgattca ttcctggcac cacattcgcg ctatgctgac
     1501 tttccggcag cgttgattcg tgattacacc gatctggaaa ttctggcaga gacggaagaa
     1561 ggggatgcat atctgtttgc cagcaaagat aagcgcattg cctttgtgac gggccatccc
     1621 gaatatgatg cgcaaacgct ggcgcaggaa tatttccgcg atgtggaagc cggactaggc
     1681 ccggaagtac cgtataacta tttcccgcac aatgatccgc aaaatacacc gcgagcgagc
     1741 tggcgtagtc acggtaattt gctgtttacc aactggctca actattacgt ctatcagatc
     1801 acgccatacg atctacggca catgaatcca acgctggatt aatcttctgt gatagtcgat
     1861 cgttaagcga ttcagcacct tacctcaggc accttcgggt gcctttttta tttccgaaac
     1921 gcacctcagg aggtgaataa attttattca tattgttatc aataagttat caagtatttt
     1981 taattaaaat ggaaattgtt tttgattttg cattttaaat gagtagtctt agttgtgctg
     2041 aacgaaaagc gcacaacgat ccttcgttca cagtggggaa gttttcgggt ccatgacgag
     2101 gagctgcacg atgactgaac aggcaacaac aaccgatgaa ctggctttca taaggccgta
     2161 tggcgagcag gagaagcaaa ttcttactgc cgaagcggta gaatttctga ctgagctggt
     2221 gacgcatttt acgccacaac gcaataaact tctggcagcg cgcattcagc agcagcaaga
     2281 tattgataac ggaactttgc ctgattttat ttcggaaaca gcttccattc gcgatacgga
     2341 ctggaaaatt cgtggtattc ccgcggactt acaagatcgt cgagtcgaga taactggccc
     2401 ggttgagcgc aagatggtga tcaacgcact gaacgccaat gtgaaagtct ttatggccga
     2461 tttcgaagat tcactggcac cagactggaa caaagtgatc gacgggcaaa ttaacctgcg
     2521 cgatgcggtt aacggcacca tcagctatac caatgaagca ggcaaaattt atcagctcaa
     2581 gcccaatcca gcggttttga tttgtcgggt acgcggtctg cacttgccgg aaaaacatgt
     2641 cacctggcgt ggtgaggcaa tccccggtag cctgtttgat tttgcgctct atttcttcca
     2701 caactatcag gcactgttgg caaagggcag cggtccctat ttctatctgc cgaaaaccca
     2761 gtcctggcag gaagcggcct ggtggagcga agtcttcagc tatgcagaag atcgctttaa
     2821 tctgccgcgc ggcaccatca aggcgacgtt gctgattgaa acgctgcccg ccgtgttcca
     2881 gatggatgaa atccttcacg cgctgcgtga ccatattgtt ggtctgaact gcggtcgttg
     2941 ggattacatc ttcagctata tcaaaacgtt gaaaaactat cccgatcgcg tcctgccaga
     3001 cagacaggca gtgacgatgg ataaaccatt cctgaatgct tactcacgcc tgttgattaa
     3061 aacctgccat aaacgcggtg cttttgcgat gggcggcatg gcagcgttta ttccgagcaa
     3121 agatgaagag cgcaataacc aggtgctcaa caaagtaaaa gcggataaag cgctggaagc
     3181 caataacggt cacgatggca catggattgc tcacccaggc cttgcggata cggcaatggc
     3241 ggtattcaac gacattctcg gctcccgtaa aaatcagctt gaagtgatgc gcgaacaaga
     3301 cgcgccgatt actgccgatc agctgctggc accttgtgat ggtgaacgca ccgaagaagg
     3361 tatgcgcgcc aacattcgcg tggcagtgca gtacatcgaa gcatggatct ccggcaacgg
     3421 ctgcgtgccg atttatggcc tgatggaaga tgcggcgacg gctgaaattt cccgtacctc
     3481 aatctggcag tggatccatc atcaaaaaac gttgagcaat ggcaaaccgg tgaccaaagc
     3541 cttgttccgc cagatgctgg gtgaagagat gaaagtcatt gccagcgaac tgggcgaaga
     3601 acgtttctcc caggggcgtt ttgacgatgc cgcacgcttg atggaacaga tcaccacttc
     3661 cgatgagtta attgatttcc tgaccctgcc aggctaccgc ctgttagcgt aaaccaccac
     3721 ataactatgg agcatctgca catgaaaacc cgtacacaac aaattgaaga attacagaaa
     3781 gagtggactc aaccgcgttg ggaaggcatt actcgcccat acagcgcgga agatgtggtg
     3841 aaattacgcg gttcagtcaa tcctgaatgc accctggcgc aactgggcgc ggcgaaaatg
     3901 tggcgtctgc tgcatggcga gtcgaaaaaa ggctacatca acagcctcgg tgcactgact
     3961 ggcggtcagg cgctgcaaca ggcgaaagcg ggtattgaag cagtctatct gtcgggatgg
     4021 caggtagcgg cggacgctaa cctggcggcc agcatgtatc cggatcagtc gctctatccg
     4081 gcaaactcgg tgccagctgt ggtggagcgg atcaacaaca ccttccgtcg tgccgatcag
     4141 atccaatggt ccgcaggcat tgagccgggc gatccgcgct atgtcgatta cttcctgccg
     4201 atcgttgccg atgcggaagc cggttttggc ggtgtcctga atgcctttga actgatgaaa
     4261 gcgatgattg aagccggtgc agcggcagtt cacttcgaag atcagctggc gtcagtgaag
     4321 aaatgcggtc acatgggcgg caaagttttg gtgccaactc aggaagctat tcagaaactg
     4381 gtcgcggcgc gtcttgcagc tgacgtgacg ggcgttccaa ccctgctggt tgctcgtacc
     4441 gatgccgatg cggcggatct gattacctcc gattgcgacc cgtatgacag tgaatttatt
     4501 actggcgagc gtaccagtga aggcttcttc cgtactcatg cgggcattga gcaagcgatc
     4561 agccgtggcc tggcgtatgc gccatatgct gacctggtct ggtgtgaaac ctccacgccg
     4621 gatctggaac tggcgcgtcg ctttgcacaa gctatccacg cgaaatatcc gggcaaactg
     4681 ctggcttata actgctcgcc gtcgttcaac tggcagaaaa acctcgacga caaaactatt
     4741 gccagcttcc agcagcagct gtcggatatg ggctacaagt tccagttcat caccctggca
     4801 ggtatccaca gcatgtggtt caacatgttt gacctggcaa acgcctatgc ccagggcgag
     4861 ggtatgaagc actacgttga gaaagtgcag cagccggaat ttgccgccgc gaaagatggc
     4921 tataccttcg tatctcacca gcaggaagtg ggtacaggtt acttcgataa agtgacgact
     4981 attattcagg gcggcacgtc ttcagtcacc gcgctgaccg gctccactga agaatcgcag
     5041 ttctaagcaa caacaaccgt tgctgactgt aggccggata aggcgttcac gccgcatccg
     5101 gcaatcggtg cacgatgcct gatgcgacgc ttgcgcgtct tatcatgcct acagccgttg
     5161 ccgaacgtag gccggataag gcatttacgc cgcatccggc aatcggtgca cgatgcctga
     5221 tgcgacgctt gcgcgtctta tcatgcctac aaccgttgcc gaacgtaggc tggataaggc
     5281 gtttacgccg catccggcaa ttctctgctc ctgatgaggg cgctaaatgc cgcgtggcct
     5341 ggaattactg attgctcaaa ccattttgca aggcttcgac gctcagtatg gtcgattcct
     5401 cgaagtgact tccggggcgc agcagcgttt cgaacaggcc gactggcatg ctgtccagca
     5461 ggcgatgaaa aaccgtatcc atctttacga tcatcacgtt ggtctggtcg tggagcaact
     5521 gcgctgcatt actaacggcc aaagtacgga cgcggcattt ttactgcgcg ttaaagagca
     5581 ttacacgcgg ctgttgccgg attacccgca cttcgagatt gcggagagct tttttaactc
     5641 cgtgtactgt cggttatttg accaccgctc gcttactccc gagcggcttt ttatctttag
     5701 ctctcagcca gagcgccgct ttcgtaccat tccccgcccg ctggcgaaag actttcaccc
     5761 cgatcacggc tgggaatctc tactgatgcg cgttatcagc gacctgccgc tgcgcctgcg
     5821 ctggcagaat aaaagccgtg acattcatta catcattcgc catctgacgg aaacgctggg
     5881 gacagacaac ctcgcggaaa gtcatttaca ggtggcgaac gaactgtttt accgcaataa
     5941 agccgcttgg ctggtgggca aactgatcac gccttccggc acgttgccct tcttactgcc
     6001 gatccaccag acggacgacg gcgagttatt tattgatacc tgcctgacga cgaccgccga
     6061 agcgagcatt gtttttggct ttgcgcgttc ttattttatg gtttatgcac cgctgcccgc
     6121 agcgctggtc gagtggctac gggaaattct gccgggtaaa accactgctg aattgtatat
     6181 ggctatcggc tgccagaagc acgccaaaac cgaaagctac cgcgaatatc tcgtttatct
     6241 acagggctgt aatgagcagt tcattgaagc gccgggtatt cgtggaatgg tgatgttggt
     6301 atttacgctg ccgggctttg atcgggtatt caaagtcatc aaagacaagt tcgcgccgca
     6361 gaaagagatg tctgccgctc acgttcgtgc ctgctatcaa ctggtgaaag agcacgaccg
     6421 tgtgggccga atggcggaca cccaggagtt tgaaaacttt gtgctggaga agcggcatat
     6481 ttccccggca ttaatggaat tactgctcca ggaagcagcg gaaaaaatca ccgatcttgg
     6541 tgagcagatt gtaattcgcc atctctatat cgagcggcgg atggtgccgc tcaatatctg
     6601 gctggaacaa gtggaaggtc agcagttgcg cgacgccatt gaagaatacg gtaacgctat
     6661 tcgccagctt gccgctgcta acatttttcc tggcgacatg ctgtttaaaa acttcggtgt
     6721 cacccgtcac gggcgtgtgg ttttttatga ttacgatgaa atttgctaca tgacggaagt
     6781 gaatttccgc gacatcccgc cgccgcgcta tccggaagac gaacttgcca gcgaaccgtg
     6841 gtacagcgtc tcgccgggcg atgttttccc ggaagagttt cgccactggc tatgcgccga
     6901 cccgcgtatt ggtccgctgt ttgaagagat gcacgccgac ctgttccgcg ctgattactg
     6961 gcgcgcacta caaaaccgca tacgtgaagg gcatgtggaa gatgtttatg cgtatcggcg
     7021 caggcaaaga tttagcgtac ggtatgggga gatgcttttt tgagtaaagc ttccatataa
     7081 tttttctccg caatgtatcg agggttatcc gtaaagccaa agctttcagc catcttattt
     7141 aatgtattaa ggattaattc agcaataacc cggtgaccca attcaaaagc caactcaaag
     7201 gcagagtatt tttgtggggc tttgtgttgc caaaaatcca taatatcttc agcggtaaat
     7261 ccaaacaggc gtgcatggtt agataaagca agataaaccg tctctacaac attttgttgt
     7321 ttatgctgta tcgctgaaaa caaaccggga tattcattag aattatttgc caggaggagg
     7381 ggcttcatat tttttttatc gaatttaaac gtattaaaca gagtgggtta tgcgttaaaa
     7441 atagtattaa taacgttcat atgtccacta ttataggcag tcaacatttc tgttctatgt
     7501 agttctggca ttttctggag ctgaatcatc agttgcgtaa gttggtgatt ctgaccaatt
     7561 gccattagca aattcatcgt tgctggtgat aatggagggg ttatttcatc cacagatcct
     7621 tttaacatca atagctgttc tttcggaagt gctggaatta ttgcaacaga ttggttaata
     7681 atatgatcat ttgaccattt caggaggcta taatctacat tcataaaatc catcagagaa
     7741 tagtgtttaa tatcctcttt gctttcgctc ataattctta ttttatcgtt agtgacatta
     7801 ggatcataaa ccgaaaccac gtaatgggta catccttcag ttgtgttctt tattcttagc
     7861 cggaccgtca aagcatggtt atccaccagt aatatggctg ccataacact gatgccattt
     7921 gattccatct tgtcgaaata tgccttcata actagcccga aatcaccaca ggcagcaaca
     7981 caacgcccct caggacggct ggaaatcaac gccaaagtct ggtttttgca ttttttgttg
     8041 aagtgagtga gaaatttttc agctgaagag taatcatctt gatggggttt gtaatcaaca
     8101 ttctgataaa tatcattaat atattgtgca gcaatgtgtc ggcatactat acggttttgg
     8161 ggtcgccccg gaaaataaca ttgaccatta agtttaattc ttgggaattc tttttctttt
     8221 ctatctgatt ttaggtttaa ttcttgttta tcaagagtgt ctgagcgttg aggtaaaatt
     8281 tccattaggt attcttcaaa tattggggca atggtttgag taagtgtcgt gttttcgttt
     8341 ttattaatta tcgtaatttc tttcttatca gcttgtaaga cggacgtgga aaatttaatg
     8401 acaaaatcct tgttgctaaa gatatgacga ccattttgtc tacagttctc tgagaagctt
     8461 tttaatagag gcgtcgccag ctccttgcca gaaaatttat cctcgagttc tttataaaac
     8521 aattcactca gggtttggtg ttcatttgtc tgggctgtat tattaatatt tgcagagaaa
     8581 gaactacgag ggatacgagt aatcatttaa atatctcatt gtttattgat gtcttgaatt
     8641 ttaactatca gaacagtgaa aaaatttaat atgattacaa ctaaagaaat atcataaatc
     8701 gctcaatctc ataatgcagc cgtaaaagtt tcggtggaat gagatcttgc gattttgtta
     8761 atgattaagt agtttgatgt ttaaaaataa tgctgagtta ataaaagtgt gtgaagtgta
     8821 tgacattaag ttattttggc gtcaatgcga ttaacagaca cccttattct attgccactc
     8881 aggtatgatg ggcagaatat tgcctctgcc cgccagaaaa agtcagcgca ttccaccgta
     8941 cgccagcgtc acttccttcg ccgctttaat caccatcgcg ccaaactcgg tcacgcggtc
     9001 atcggtaata cgtgaaatcg gtccggaaat tgaaattgcg gcaaacggtt cgcggtgctc
     9061 atcgaaaata cacgctgcaa ggcaacgtag ccccagtgca tgttcctcat cgtcaaatga
     9121 ataaccccgt ttgcgcgttt gggcgagatc ttcttttaaa tgcacaggag acaccagcgt
     9181 tgcgtgggta taggcatgta accctttgcg gtgcagcagc ttcgtcacct gttcttcgct
     9241 cagttgggct aaaaaggctt tacccgcacc ggaagcgtgc atcggcaatt taccgccgat
     9301 aggcgcggac attcgcatca gatgcgtaca ctgtacctgg tcgataataa tcgcttcgtg
     9361 atcgctttga tcaagcaccg ccatattgac cgtttcgcca gactcttcca ttaaattgcg
     9421 caggataggg tgaacaatcg ctaacaaatt acggctctgg agaaagctgc tgccgaccat
     9481 aaaggcatgt gcgccgattg cccaatgtcc cagttcgccg acctgacgca cgaaaccctg
     9541 ctgttgcatc gtggttagca ggcggtgggt cgtggaattg ggtaacccgg cttgttgcgc
     9601 cagctctgtg agtgccacac tgccattgga ttcggcaatc cactccagta atttcaggcc
     9661 acgcgttaaa gactgaacct gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc
     9721 gcgtttcgcg ggaatgggtg cgaccatgac agcctccttt ttctgtatcg tggaaatcat
     9781 tttcattttt attgttagct aatgcaatag ttgctgaact gatccgatga gttaatgttg
     9841 aacaaatctc atgttgcgtg gtggtcgctt ttaccacaga tgcgtttatg ccagtatggt
     9901 ttgttgaatt tttattaaat ctgggttgag cgtgtcggga gcaagtgtga gcagcaaagt
     9961 ggaacaactg cgtgcgcagt taaatgaacg tattctggtg ctggacggcg gtatgggcac
    10021 catgatccag agctatcgac tgaacgaagc cgattttcgt ggtgaacgct ttgccgactg
    10081 gccatgcgat cttaaaggca acaacgacct gctggtgctc agcaaaccgg aagtgatcgc
    10141 cgctatccac aacgcctact ttgaagcggg cgcggatatc atcgaaacca acaccttcaa
    10201 ctccacgacc attgcgatgg cggattacca gatggaatcc ctgtcggcgg aaatcaactt
    10261 tgcggcggcg aaactggcgc gcgcctgtgc tgacgagtgg accgcgcgca cgccggagaa
    10321 accgcgctac gttgccggtg ttctcggccc aaccaaccgc acggcgtcta tttctccgga
    10381 cgtcaacgat ccggcattcc gcaacatcac ttttgaccag ctggtggcgg cctatcgcga
    10441 atccaccaaa gcgctggtgg aaggtggtgc ggatctgatc ctgattgaaa ccgttttcga
    10501 cacccttaac gccaaagcgg cggtttttgc ggtgaaaacg gagtttgaag cgctgggcgt
    10561 tgagctgccg attatgatct ccggcaccat caccgacgcc tccgggcgca cgctctccgg
    10621 gcagaccacc gaagcatttt acaactcatt gcgccacgcc gaagctctga cctttggcct
    10681 gaactgtgcg ctggggcccg atgaactgcg ccagtacgtg caggagctgt cacggattgc
    10741 ggaatgctac gtcaccgcgc acccgaacgc cgggctaccc aacgcctttg gtgagtacga
    10801 tctcgacgcc gacacgatgg caaaacagat acgtgaatgg gcgcaagcgg gttttctcaa
    10861 tatcgtcggc ggctgctgtg gcaccacgcc acaacatatt gcagcgatga gtcgtgcagt
    10921 agaaggatta gcgccgcgca aactgccgga aatccccgta gcctgccgtt tgtccggcct
    10981 ggagccgctg aacattggcg aagatagcct gtttgtgaac gtgggtgaac gcaccaacgt
    11041 caccggttcc gctaagttca agcgcctgat caaagaagag aaatacagcg aggcactgga
    11101 tgtggcccgc cagcaggtgg aaaacggtgc gcagattatc gatatcaaca tggatgaagg
    11161 gatgctcgac gccgaagcgg cgatggtgcg ttttctcaat ctgattgccg gtgaaccgga
    11221 tatcgctcgc gtgccgatta tgatcgactc ctcaaaatgg gatgtcattg aaaaaggtct
    11281 gaagtgtatc cagggcaaag gcattgttaa ctctatctcg atgaaagagg gcgtcgacgc
    11341 ctttatccat cacgcgaagc tcttgcgtcg ttacggtgcg gcagtggtgg taatggcctt
    11401 tgacgaacag ggacaggccg atacccgcgc acggaaaatc gagatttgcc gtcgggcgta
    11461 caaaatcctc accgaagagg ttggcttccc gccagaagat atcatcttcg acccgaacat
    11521 cttcgcggtt gccaccggca ttgaagagca caacaactac gcgcaggact ttatcggcgc
    11581 gtgtgaagac atcaaacgcg aactgccgca cgcgctaatt tccggcggcg tatctaacgt
    11641 ttctttctcg ttccgtggca acgatccggt gcgcgaagcc attcacgcag tgttcctcta
    11701 ctacgctatt cgcaatggca tggatatggg gatcgtcaat gccgggcagc tggcgattta
    11761 cgacgacctg cccgctgaac tgcgcgacgc ggtggaagat gtgattctta atcgtcgcga
    11821 tgatggcact gagcgtttac tggagcttgc cgagaaatat cgcggcagca aaaccgacga
    11881 caccgctaac gcccagcagg cggagtggcg ctcgtgggaa gtgaataaac gtctggaata
    11941 ctcgctggtc aaaggcatta ccgagtttat cgagcaggat accgaagaag cccgccagca
    12001 ggctacgcgc ccgattgaag tgattgaagg cccgttgatg gacggcatga acgtggtcgg
    12061 cgatctgttt ggcgaaggga aaatgttcct gccacaggtg gtcaaatcgg cgcgcgtcat
    12121 gaaacaggcg gtggcgtatc tcgaaccgtt tattgaagcc agcaaagagc agggcaaaac
    12181 caacggcaag atggtgatcg ccaccgtgaa gggcgacgtc cacgacatcg gtaaaaatat
    12241 cgttggtgtg gtgctgcaat gtaacaacta cgaaattgtc gatctcggcg ttatggtgcc
    12301 tgcggaaaaa attctccgta ccgctaaaga agtgaatgct gatctgattg gactttcggg
    12361 gcttatcacg ccgtcgctgg acgagatggt taacgtggcg aaagagatgg agcgtcaggg
    12421 cttcaccatc ccgctattga ttggaggcgc gacgacctca aaagcgcaca cggcggtgaa
    12481 aattgagcag aactacagcg gcccgacggt gtatgtacag aacgcatcgc gcaccgttgg
    12541 tgtggtggcg gcgctgcttt ccgatactca gcgtgatgat tttgtcgctc gtacccgcaa
    12601 ggagtacgaa accgtacgta ttcagcacgg gcgtaagaaa ccgcgcacac caccggtcac
    12661 gctggaagcg gcgcgcgata acgatttcgc ttttgactgg caggcttaca cgccgccggt
    12721 ggcgcaccgt ctcggcgtgc aggaagttga agccagcatc gaaactctgc gtaattacat
    12781 cgactggaca ccgttcttta tgacctggtc gctggccggg aagtatccgc gcattctgga
    12841 agatgaagtg gtgggcgttg aggcgcagcg gctgtttaaa gacgccaacg acatgctgga
    12901 taaattaagc gccgagaaaa cgctgaatcc gcgtggcgtg gtgggcctgt tcccggcaaa
    12961 ccgtgtgggc gatgacattg aaatctaccg tgacgaaacg cgcacccatg tgatcaacgt
    13021 cagccaccat ctgcgtcaac agaccgaaaa aacgggattc gctaactact gtctcgctga
    13081 cttcgttgcg ccgaagcttt ctggtaaagc agattacatc ggcgcatttg ccgtgactgg
    13141 cgggctggaa gaggacgcac tggctgatgc ctttgaagcg cagcacgatg attacaacaa
    13201 aatcatggtg aaagcgcttg ccgaccgttt agccgaagcc tttgcggagt atctccatga
    13261 gcgtgtgcgt aaagtctact ggggctatgc gccgaacgag aacctcagca acgaagaact
    13321 gatccgcgaa aactaccagg gcatccgtcc ggcaccgggc tacccggcct gcccggaaca
    13381 tacggaaaaa gccaccatct gggagctgct ggaagtggaa aaacataccg gcatgaaact
    13441 cacagaatct ttcgccatgt ggcccggtgc atcggtttcg ggttggtatt tcagccaccc
    13501 ggacagcaag tactacgcgg tggcgcaaat tcagcgcgat caggttgaag actatgcccg
    13561 ccgtaaaggt atgagcgtct ccgatgttga gcgctggctg gcaccgaatc tggggtatga
    13621 cgcggactga ttcacaaatc tgtcactttt ccttacaaca aacagggcgc tcaatgagtg
    13681 ccctgtctct ttattaatat gaaacactta tactggaaac aggctggaat aatcttagcc
    13741 ggaaaaacga atgtcaaaag gcacagtaaa gatgccagtc agcgtgtttc cggtcctttg
    13801 gtcattcaat tgcttcagga acatataacg ataaggagaa cctgaactcg tgttaacgct
    13861 gcttcacctg ctttctgccg tcgccctgct ggtctggggg actcatattg ttcgaaccgg
    13921 cgtaatgcgc gtcttcggcg cgcgtttgcg tactgtcctt agccggagcg tcgaaaaaaa
    13981 gccgctcgcc ttttgcgcgg ggatcggcgt taccgcactg gtgcagagca gtaatgccac
    14041 caccatgctg gtgacctcgt ttgtcgctca ggatctggta gccctcgcac cggctctggt
    14101 cattgtgctg ggggcagatg tcgggacggc gctaatggcg cgtattctca ccttcgactt
    14161 atcctggctg tcaccgttac ttatttttat cggcgtgatt tttttcctcg gacgcaaaca
    14221 gtcacgcgcc gggcaactgg gccgcgtcgg tattggtctt gggctgattt tactggcgct
    14281 ggagttgatt gtgcaggccg taacgccgat cacccaggca aacggcgttc aggtgatctt
    14341 tgcctcgctg accggcgata ttctgctgga tgcgctgatt ggcgcgatgt tcgccattat
    14401 cagctactcc agccttgctg ctgtactgct gactgcgact ctgaccgccg caggcattat
    14461 ctccttcccc gtggcgctct gtctggtgat tggtgctaac ctcggttccg gcctgctggc
    14521 gatgctcaac aacagtgccg ccaatgccgc agcccgccgt gtcgcgctgg gtagtctgct
    14581 gtttaagctg gtgggtagcc tgattatcct gccgtttgtc catttgctgg cagagacaat
    14641 ggggaagctg tcattgccaa aagcggaact ggtgatctat ttccacgtct tctacaacct
    14701 tgtacgttgc ctggtcatgc tgccatttgt tgacccgatg gcacggtttt gcaaaacgat
    14761 tattcgcgat gaaccggaac tggataccca gctacggcct aaacatctgg atgtcagcgc
    14821 gctggatacg cccacgcttg ctctggcgaa cgccgcgcgc gaaaccctgc gcattggcga
    14881 cgccatggaa cagatgatgg aagggctgaa taaagtgatg cacggcgagc cacggcagga
    14941 gaaagagctg cgtaagctgg cagatgatat caacgttctc tataccgcca ttaagctgta
    15001 tctggcgcgg atgccaaaag aggaactggc agaagaagag tcgcgtcgct gggcggagat
    15061 tattgaaatg tcgctcaacc ttgaacaggc ttccgatatc gtcgagcgca tgggcagcga
    15121 aattgccgat aaatcgctgg cagcacggcg ggcattttcg cttgatgggt tgaaggaact
    15181 ggatgcgctc tatgagcaat tgctcagtaa tttaaagctg gcaatgtcgg tgttcttctc
    15241 tggcgatgtc accagcgctc gtcgtttgcg tcgcagcaaa catcgttttc gcattcttaa
    15301 tcgccgctat tcccacgccc acgtcgatcg cctgcatcag caaaacgtgc agagcattga
    15361 aaccagttcg ctacatttag gcttactggg agatatgcag cgcctgaact cgctgttttg
    15421 ttcggtggct tacagtgtgc tggaacagcc ggatgaagat gaaggacggg acgagtatta
    15481 atattgtgaa tcctgtaggc ctgataagac gcgtcaagcg tcgcatcagg cactatgaac
    15541 aagatgccgg atcatgcatc cggcaattta cttaaaaacg gtgaccagct tctagcggca
    15601 ccgcttcttc accagcctta aacacataag tggtgttcgg gccacccagc gtagcgtgac
    15661 ctttactcac tgtgatccag ttaccttccg gtagaccaat aatcgtcagt tctggcgcga
    15721 cgaccagcag ttcgcgaata cgctgctcac gggtttcacc tttatggcct tccggcagcg
    15781 cgttggtgaa gtgcgggttg atttgcagcg ggaacagatt tagcgcatcg aaaccttgcg
    15841 gatcgacaat cggcatatcg ttggtggtac gaatggttgg gcaagcaagg ttagcgcctg
    15901 cgctccagcc aatatacagc gcaccacgtt taaccacgtc agtaattggt gccagcagcc
    15961 cgcgctcgcg gcactgtttc agcaactgga aagtattccc gcccccgaca atcacgatct
    16021 cagcattttc aatcgcggca acgggatcga caacgctatg aataccggtg acagatacac
    16081 ccagcggagc gagaaccgca gccgttttcg ctgtgtaatc atcccaggtc tgcgttacgc
    16141 cagcgaaagg aataaatacc gctgaacggc gaccctgcaa ctgttcagca atcagcggca
    16201 gcgcatgttc cagccaggct ttacccggca gcgtcgagtt actcaataaa agcagttcca
    16261 ttacttctcc agttagtaag aaatcacggg aggcatgcta ccactgcgaa tttaaccact
    16321 tactgacatg gctcacgatg tcacgtattg caccgggcgc tgagactgat gacaaacaca
    16381 gcattgcctg atgcgctacg cttaccaggc ctaccagata actgcaatat attgaatttt
    16441 cgagattatg taggccgaat aaggctttat tccgcatccg gcataaacaa tgtgtacttt
    16501 gccaccaatt tgagcgcccc ttaaggcgct gatgtttttt tatccttctc gtgcacattg
    16561 ggccgcttca gcaatcagcc acacatcagg tcgcgaagtt gccgcggcga ccagccagcg
    16621 acatgcagag ttaagtagcg cgattaatca tgggaaattg cctctgcatg agtgagcaga
    16681 tattgctccg cctcaccgga ccagatcccc tgatgacgcg ccagaatcgc cgccagtgct
    16741 tgatcctgaa tctgcgtgag tgacgtcagc ggcaggtatt tcccggtata caccagcttt
    16801 ttgccgccgc cgacggcagg caattcaagc gtggtttcgc ccgcggcatt cagcccaaga
    16861 atatgtgtta ccacttttgc ggcctgcact tttttctctt caatcagttt gaccgccgcg
    16921 cgcatgtcgt cggtattgcc gcctgacgtg cccacgtagt gggtaaatgc ataatgcaca
    16981 tcgtagaaat taattggcgc gctgaaatgt ttatcctgcg gtccggcgaa gaaattcagg
    17041 caaccatctg tcgccagcaa ggaagaggcg agagtcacca gtccttcatt aggcacaaag
    17101 acgaaaatat catcgaagcc gtgaccgcca ctcagcgcca ttagcgtatc aaatgctgca
    17161 tcggcggcat tgagataatg aatcagtgtt tgcggttctg atggatagtg cttgcgcgca
    17221 taactcaatt tatcgttgtc ggtatcggta atgacgagca gcgacgggtt aacgggtcca
    17281 tgtagcgcat aatcaatcgc caacagtccc attggtccgg tgccgccgag gatcagcatg
    17341 cgcccttgcg ggcgaatccc catcgtgtgg ttataactac cttcctgaag atgatagttg
    17401 gcgttgaacg cgccaatcac gcaggaaagc ggttcaacca gtgagccttc aaaataggtt
    17461 tcgccgtcgt atgccagcag gcaatcttgt tccatgacct cgttgggaat aaccacatgc
    17521 gtggcttcgc cgcccaccca cgggaaggag tagccggggc agtccgggcg gtcggggagt
    17581 tgcaggttgg cctgaatcac ataacgctga cccggctgga atttgtgctg ccacttttta
    17641 cccacggcca gaatatcgcc gcaaaactcg tggccgatga tgatggggtt ggtcgccaca
    17701 tcgtcgggta cttttttatg gttttcaccc agattggcct ctttccagga agagaggcac
    17761 aggctgtcag tgaccaccgt cgcgaggatt tcatcctcct gcatttcagg aaggtcaaag
    17821 gtttccaggc gtaaatcacg tttaccataa agacgcagag ctgttgtttt cataaaccac
    17881 ctcatgttga gcgatggccc cggcagtcgt gccggggcga agaatcagga caggaagccc
    17941 agcgcattac cgataatccc taagccaaac agggcgaaaa tcagccatac cgggttaatt
    18001 tttttgttga gcagacgaac cataagcagc gtcagaccga gcgccagcaa accagggcaa
    18061 agttggtcga gaatgttctg cacggtcatg gtgacggtgg agccatcggc ggcatgcgtt
    18121 tgtgaaacca ccaacggtac gttgattgac gtccatttgg tgaccagcac gcccatcaca
    18181 aacagtccga gaatcgacgc gccttcggtg agtttttgca gcacattccc gcccatatcg
    18241 ctgacgatat tcaccccttt gcgaaagccg agctgtaggc cataccactt catcgccaga
    18301 cgcaccgcat tgaaaataaa gaaaaaaaaa gcgggccgag gatgttgcca gaaagtgcca
    18361 gagatgcgcc gagcgcagcg gtaatcgggc gcagcgttcc ccaaaccagt ggatcgccaa
    18421 cccctgccaa tggtcccatc agaccgactt tgatgccgtt aatggtaccg tcatcaattt
    18481 ccgcgccgtt ggcccgcgcc tcttccatgg cggcagtgac gccgatgacc gggccacata
    18541 cggctggcgt ggtattgaag aacaccaggt gtcgcctgag cgccgcaacc tgatcctctt
    18601 ttaatgggta aagtcgcttg atggcgggga tcatgtcgta gcaaaagccc agcccgtgaa
    18661 tacgttcaaa gttaaaggac gcctgttgca ggttggagcg cagaaacatg ctcaccagat
    18721 cgctgcgtgt aatttttctc tgttccatga tggctccgtt aatcgtcaag ttggtcaagg
    18781 gcggtggagg tggtggcctg gggttgaggt tcagctttac gccactgtgg atttagctgg
    18841 atgtagacca gggccatgat cacgccgacg ccaccgaacg ccagcagact gagatcgagg
    18901 tagccacctg cgaggaaacc gaggaaaaag aaaggcatca aatatttcac gcccatcata
    18961 cgtagcacca tggcgtaacc gaccaccacg ataaaaccgc cagcaatttg cagaccgcgc
    19021 gtcacgaatt ccggtatcgc gctcagcata ttgctcacca tatcggcgct gacgaacagt
    19081 gagacaatca gtgccggaat agcaacgcgc agcgcctgca cgccaagcgc ggagacatgc
    19141 agaatatcga gagtgcgaaa ccgcgcttct tccgctgctt tatccgccgc gtgctggaac
    19201 actacggtga tggtacgggc aaacacagtc agcacctggc ctgccgcagc cacaggcaac
    19261 gcgatggcga ttccggtggc gatgctttgc tgaccaacga taaccagaat ggcggagatt
    19321 atgctggcga gtgcagaatc cggagattgc gccgcgccga cgttcatcca gccgagagct
    19381 atcaattcca gcgtaccacc gagtattatt ccggttttta aatcaccgag aattaaacca
    19441 atcaccgtac aggcaattaa cgggcgatga gtctgaaatt catccagcac gctgcccatt
    19501 ccggcaatac aggaaaaaag aaatatagca atgatttgta gggtacttat ttccatgatt
    19561 tttcaccttt taagtgtcgt catccctgcc tgttaaagca tatgtacaca ggcgctatta
    19621 tttaatttgc gattaactgt tcgtttattt tgtcgataat attgattgag ggatctgacg
    19681 ctacgacgcg taaatccagt atgacgccaa gattatttaa ctcatgaaat gcgttaatat
    19741 catcatcatc taatgaaacc gctttggtta actgtttttt accgggccgc caggccatgc
    19801 cgccaatatt cagggtgtct atttttaccc cctggcgaac catcgctaag gcatcctgtg
    19861 ggcgagtgaa aagataaaaa acggtttcgt cctgatattg cgggttgtga taaacagcga
    19921 cggctttttc aatattgacc acgttaacct tcattcccgg tggcgcagcc tgacgcaata
    19981 atgtccggcg aacttcatcg ttataaactt cgtcattgca gataataatg cgctgggcgt
    20041 tagccacttt cgaccagacg gtagtgacct gaccgtggat caggcggtca tcaatgcggg
    20101 cgagcgtaat gttcatcaga aatcctcctc tgccgttgcc agttgtttcc agacaacgca
    20161 ggtttgcttt gcgatttgcg tcaggtgttc acacagttca tcaacattca tgctgtcctg
    20221 gttatcaacc agttcgagcg ccagcggcag agaaaggccg ctgattaccc gcagacgagg
    20281 attgcgcatc gcaagcatcg ctgccgcatt ccacgggctg ccgcactgta aatccacggc
    20341 aatcagccac tcatcttgat tgtgaatgct tactaacttt tccagctttg ctacgatgtc
    20401 tccggcgttc tcgccgggca caaattcaac ggcttcaaca ttggcgttgc cgtaaaccat
    20461 ctgaaccgat tccagcatgg cgcaagctaa tttgccgtgg gcacaaaaaa tggcgttgac
    20521 cataaagcct ccttagccgc gcgttttgcc gcccgcaatg ttagtggtta ctccggtgat
    20581 gtagctggcg cgttcagaca acagataaca aacaaaatca gcgacttctg ctaatcttcc
    20641 ggcgcgccca ataggaatgg cgtttttggt atagccttca cgcagctgct cgacggtgat
    20701 attgcgcgtc cacgccagcg cctcttcata ttccggcgtg cgtagcccgg ttttttccag
    20761 aatcccgggc gcgataccga ccacacggat accgtgttta cccagctctt tcgaccagga
    20821 gcgcgtgaag ctattaagcg cggctttggt agcggcgtaa cagctttggc cttctgagcc
    20881 ttccagtcca ctttctgagg aaacattcac aatcacgcca tcatgttgtt tgaccatctg
    20941 tcgcgccacc gcctgcgaca tcagaaaaac gcctttctgg ttgatattga ccattttttc
    21001 gaatgcagct tcgttgagtt catactgccc ggcaggcgct ttctcatcga ccagcagacg
    21061 cgggaaattg accccggcgt tattgaccag accgtcgatg cgaccaaaac gctggataat
    21121 ttccgctacc gtatgattta cctctttggc gctggaaata tcggtcggcc aaaactgata
    21181 gcctttatga ccttcatatt gaccatcgcc accgtgaata tctaccatct gtacgtttgc
    21241 gccttgtgct aataattcct ctacaatcgc cagaccaata ccggatgcgc cgccggtgac
    21301 gataatgatt ttatcctgca aatttaacca cgtttgcata acagactcct gattttagat
    21361 aatagggacc cgccgtaatt tttattacgg taaacatcgg gaagattttt atttcagtaa
    21421 tagttcagct gtgctgctat tcgttaccag acaattaata tattttccac gcagtgcacc
    21481 aataattccg ctgtattttt cttcactcat ggcaatgcca atggaatatc gcgcctgctt
    21541 taatttattc atttcgatag agagcgtttt ttcgctcata ttcgtttcaa ccattgcgcc
    21601 gtgaatatca aaaaagcgcg agcaaatatc gccagcaacc tggcgggcat tcaggtcgtc
    21661 actctcttca ccaccataaa acgcatgcca gttagcgccg tcgcgaatgg ccggtgagcc
    21721 aattcccacc agggcgacat ccagattatc ccagtaggca gagatggttt taaagtgctg
    21781 agactgcatg atcccattac gaattaatgg gttatccagc agagccggaa aatccgcgag
    21841 atgcgattcc cctttcagct tcgctgccgc gctgtaggtt aatgtgttta catgatagcg
    21901 gctttcgagt ttaccggacg gtccgccaat aattggtacg cagattaact gccgcgattg
    21961 ccccgcctgc ggcaagtttt caaccagtgc gctcaccgcg cgaccccagg aaaaaccgac
    22021 aatatcgcca ggctccagca agcgatccag cagttgcgcg ccgtgtaacc ccatcatcgc
    22081 cagttgagtc tcttcatcct catcatttcc cgacaccacc acaacgtctt tcaggccaaa
    22141 cttttgcttc agttgctgct ccagccagag attttcgttg tagtcatagt tgatggcgat
    22201 ggtgacaatt ccctgatcgc ggcctcgttt aagcaagcgg ctgatggtgg tgcggtaaat
    22261 acgcagttcg cgcgcgattt gcgcctgcgt catatcctgt tcgtaataga gttgggcaat
    22321 cttcacaatc aaacggatat cgtcactgtt ttccattgcc gcctcctggt tctgcacatt
    22381 tgtgcaaata cgctttctca ccgcttgatc tgatataacc ccatcatgag ggcctttcaa
    22441 agcgattttt cactctttgc atggggagaa atagcgcttt ctcttgcaca tttgtttctc
    22501 tgcgtagtag tcatcagcac aggtgtgcag cgattcgtaa tatgttgatt atccttattt
    22561 tgccaggcaa atttgcatcg tacttccgga gacacttttg tgataaaagg cgtggcagat
    22621 cacatttaat ttcgacgttg ccgcataacc ctgtttttcc ccgctaagag gtacggtata
    22681 ttccgcgttt tacaggagaa tttatgctgc ccgactcatc agtccgttta aataaataca
    22741 tcagcgaaag cggaatttgc tcacgccgcg aagcggatcg ctatatcgag caaggcaatg
    22801 tgttccttaa tggcaagcga gccaccattg gcgatcaggt gaaacccggc gacattgtga
    22861 aagtaaacgg tcagttgatt gagcctcggg aagccgaaga tttggtactt atcgccctga
    22921 acaagcccgt cggtattgtc agcaccaccg aagatggcga gcgcgataac attgtcgatt
    22981 tcgttaacca cagcaaacgc gtgttcccga ttggccgcct ggataaagac tcccaggggc
    23041 tgattttcct caccaatcac ggcgatctgg tgaataagat cctccgtgcg ggtaacgatc
    23101 atgagaaaga gtatctggtg acggtcgata aaccgattac cgatgaattt attcgtggta
    23161 tgggggcggg ggtgccgatc ctcgggacgg tgacgaaaaa gtgcaaagtc aaaaaagaag
    23221 cgccgtttgt cttccgcatt accctggtgc aggggctgaa ccgtcagatc cggcgcatgt
    23281 gcgagcattt cggctatgaa gtgaaaaagc tggaacgtac gcgcattatg aacgtcagtt
    23341 taagcggcat tccgctgggt gagtggcgcg atttaactga cgatgagtta atcgacctgt
    23401 ttaaactcat tgaaaattcc tcttccgagg taaaacctaa agcgaaggct aaaccgaaaa
    23461 cagcgggcat caaacgtcca gtcgttaaga tggaaaaaac ggcggaaaaa ggcggtcgcc
    23521 cggcgtccaa cggtaagcgt tttacctcgc cggggcgtaa aaagaaggga cgctaatcag
    23581 cgcctgccgc gcgtcgacgt atttgctgac cagctaaatg acgcttcagg atccggttta
    23641 taagcctgct ttttcttcaa ctggcgggct tttttcgctt cagcctcacg ctcgaccatt
    23701 aatttatcga tgtactcttt cttaatgctg ttggtttcgg agttggtcag tacgcgacca
    23761 tgcgcaatcc gcgcccgatc cagcagtgtt ttaagttcac gctgctcgcg ttcagtcatc
    23821 tctttttggg taatacgggg gagtgccatg atggtgccct cagtgagcca gagagttcag
    23881 tgtacgggaa ttcgtttgcg agctgaactg ataatattcc tttgttctct tcttaaatat
    23941 ttatctttc
//
LOCUS       DAZFJF010000074        22931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFJF010000074 DAZFJF010000000
VERSION     DAZFJF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.074"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            213..308
                     /locus_tag="ACHKAU_003100"
                     /pseudo
     CDS             213..308
                     /locus_tag="ACHKAU_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            332..4846
                     /locus_tag="ACHKAU_003101"
     CDS             332..4846
                     /locus_tag="ACHKAU_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO5532112.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4847..6496
                     /gene="yfaQ"
                     /locus_tag="ACHKAU_003102"
     CDS             4847..6496
                     /gene="yfaQ"
                     /locus_tag="ACHKAU_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO5532113.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6501..7277
                     /gene="yfaP"
                     /locus_tag="ACHKAU_003103"
     CDS             6501..7277
                     /gene="yfaP"
                     /locus_tag="ACHKAU_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO5532114.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7552..10401
                     /gene="rcsC"
                     /locus_tag="ACHKAU_003104"
     CDS             7552..10401
                     /gene="rcsC"
                     /locus_tag="ACHKAU_003104"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO5532115.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHICIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10487..11137)
                     /gene="rcsB"
                     /locus_tag="ACHKAU_003105"
     CDS             complement(10487..11137)
                     /gene="rcsB"
                     /locus_tag="ACHKAU_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO5532116.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(11154..13826)
                     /gene="rcsD"
                     /locus_tag="ACHKAU_003106"
     CDS             complement(11154..13826)
                     /gene="rcsD"
                     /locus_tag="ACHKAU_003106"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO5532117.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            14565..15656
                     /gene="ompC"
                     /locus_tag="ACHKAU_003107"
     CDS             14565..15656
                     /gene="ompC"
                     /locus_tag="ACHKAU_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5532118.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            15768..16823
                     /gene="apbE"
                     /locus_tag="ACHKAU_003108"
     CDS             15768..16823
                     /gene="apbE"
                     /locus_tag="ACHKAU_003108"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO5532119.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            16897..17961
                     /gene="ada"
                     /locus_tag="ACHKAU_003109"
     CDS             16897..17961
                     /gene="ada"
                     /locus_tag="ACHKAU_003109"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO5532120.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            17961..18611
                     /gene="alkB"
                     /locus_tag="ACHKAU_003110"
     CDS             17961..18611
                     /gene="alkB"
                     /locus_tag="ACHKAU_003110"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO5532121.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            18687..20330
                     /gene="yojI"
                     /locus_tag="ACHKAU_003111"
     CDS             18687..20330
                     /gene="yojI"
                     /locus_tag="ACHKAU_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO5532122.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            20548..22194
                     /gene="mqo"
                     /locus_tag="ACHKAU_003112"
     CDS             20548..22194
                     /gene="mqo"
                     /locus_tag="ACHKAU_003112"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO5532123.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(22343..22852)
                     /gene="eco"
                     /locus_tag="ACHKAU_003113"
     CDS             complement(22343..22852)
                     /gene="eco"
                     /locus_tag="ACHKAU_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO5532124.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         5547 a         5629 c         6141 g         5614 t
ORIGIN      
        1 tgctcttctg attggctgta tgtttgatga caaaaaatta gcgcaagagg acaaaaaatc
       61 accttgcgct aatgctctgt ctcaggtcac taatcacatc taactcattg attaattgtg
      121 actctaaatg tagtgttttg tcgcgttttg cagtatgtta tttaatctat tatatgaata
      181 acataatgat ttttaatgta tttttgtaat ttactctcct tctgacatgg aaggacaccc
      241 gatggatacc caacgattcc aatcccaatt tcattggcat ttatcgttta aattttctgg
      301 cgcgatagca gcgtgcttgt ccctgtctct tgtgggaaca gggcttgcta atgctgatga
      361 ttcgcttcct tccagtaact atgcgccgcc cgccggggga acattctttt tgcttgctga
      421 cagcagtttt agcagcagtg aagaggcgaa agtgcgactg gaagcgccgg ggcgtgatta
      481 tcggcgctat cagatggaag agtacggcgg cgtggacgtt cgcctgtatc gtattcctga
      541 cccgatggca tttttgcgcc agcagaaaaa cctgcatcgc attgtggtgc aaccgcaata
      601 tctgggcgac gggctgaaca atacgctgac ctggctgtgg gataactggt acggcaaatc
      661 tcgccgcgta atgcagcgta ctttctcttc tcagtcacgg cagaatgtga ctcaggcatt
      721 acccgaatta catctcggca atgccattat taaaccttcc cgttatgtac agaacaacca
      781 gttttccccg ctgaaaaaat atcccttggt ggaacagttc cgttatccac tatggcaggc
      841 taaaccggtc gagccgcagc aaggggtaaa actggaaggc gcatccagca atttcatctc
      901 gccgcagccg ggtaacattt atattcctct cggccaacaa gagccgggac tgtacctcgt
      961 cgaggcgatg gttggtgggt atcgggcgac gacggtggtg tttgtttccg ataccgtggc
     1021 gcttagcaaa gtgtcaggca acgagcttct ggtgtggacc gcgggtaaaa aacagggtga
     1081 agcgaagccc ggctcagaga tcttgtggac tgacggtctt ggcgtgatga cccgcggtgt
     1141 gaccgatgac agcggtacct tgcagttaca acatatatcg ccagaacgtt catacattct
     1201 gggtaaggat gctgaaggcg gtgtttttgt ctccgagaac ttcttctacg aaagcgaaat
     1261 ctacaacacc cgcttgtata tttttaccga tcgcccgcta tatcgcgcag gcgatcgtgt
     1321 cgatgttaaa gtgatgggcc gcgagttcca cgatccgttg cattcatccc ccatcgtcag
     1381 cgccccggcg aagctttcgg tgctggacgc taacggcagt ctgttgcaaa ccgtcaatgt
     1441 cacgctggat gcgcgcaatg gcgggcaggg aagtttccgc ctgccagaaa atgccgtagc
     1501 cggaggttat gagttacgtc ttgcttaccg caatcaggtc tatagcagca gttttcgcgt
     1561 ggcaaactac atcaagccac atttcgagat tggtttagct ctcgacaaaa aagagttcaa
     1621 aactggcgaa gcggtcagcg gcaaactgca actgctttac ccggatggtg agccggtaaa
     1681 agatgcccgc gtgcagttaa gtttgcgcgc tcagcaatta tcaatggtcg gtaacgattt
     1741 gcgttatgcc ggacgttttc ccgtgtcgct ggaaggcagc gaaacggtgt ccgacgccag
     1801 cggtcatgtg gcgttaaatc tccccgccgc cgataaaccg agccgctatt tgttaaccgt
     1861 ctccgccagt gacggcgcgg cgtatcgcgt caccaccacc aaagagatcc tcattgaacg
     1921 cggcctggcg cattactctt taagtaccgc cgcacaatac agtaatagcg gcgagtcggt
     1981 tgtgttccgt tatgccgcgc tggaatcttc aaaacaggtt cctgttacgt atgaatggtt
     2041 gcgtctcgaa gaccgcacga gccatagcgg agagctaccg tcaggcggca aatcctttac
     2101 cgtcaatttc gctaaacctg gcaactacaa tctgacatta cgcgataaag acggcttaat
     2161 tctcgctggg ttaagtcatg ccgtcagcgg taagggcagc acggcgcata ctggtacggt
     2221 agatatcgtg gcggataaaa cgctgtacca gccaggcgaa actgcgaaga tgctgattac
     2281 ctttccggag ccaattgatg aagcattatt gacgctggaa cgcgatcgcg tggaacagca
     2341 gtcgctgctt tcgcatccgg caaactggct gacgttacaa cgtttaaacg atacccagta
     2401 tgaagcccgg gttccagtga gcaattcctt tgcgcctaac atcacttttt cggtgctgta
     2461 tacccgtaat ggtcagtaca gttttcagaa cgccgggatc aaagttgccg ttcctcagct
     2521 tgatatccgg gtgaaaacgg acaaaaccca ttaccagcct ggtgaactgg tcaatgtcga
     2581 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
     2641 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
     2701 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct acgaccaggc
     2761 gctctccagc gagccggttg cgcctggcgc aactaaccgc agcgagcggc gagtaaaaat
     2821 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
     2881 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
     2941 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
     3001 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
     3061 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
     3121 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
     3181 ttcgctgacg cagaacattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
     3241 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
     3301 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
     3361 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
     3421 tgcgttagtc gatggaccat ggggtggcgt gatcaacacc ggtagccgtc tgatcccgct
     3481 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
     3541 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     3601 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     3661 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     3721 cgacagttat gccgagcagg cggataacat gccgttattg catcgggcgc tggtgctggc
     3781 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaagggttgg atgaagctat
     3841 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     3901 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     3961 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     4021 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     4081 tagcggtggt gacgctaccc agacggccgc tattttaagt ggtttgaccg ctgagcaatc
     4141 cactattgag cgcgcgctgg ccatgaactg gctggcgaaa tatatggcga caatgcctcc
     4201 agttgttttg cctgcgcctg cgggcgcatg ggctaaacat aagttaactg gagggggcga
     4261 agactggcgt tgggttggtc agggcgtgcc ggacattctc tcttttggtg acgaattatc
     4321 gccgcaaaat gtgcaggtcc gctggcgtga gccggcaaaa atggctcaac aaagtaacat
     4381 tccggtgacc gttgaacgcc agttgtatcg gcttatccct ggtgaagaag agatgagctt
     4441 tattctgcaa ccggtgacca gcaatgagat tgacagcgat gcgctgtatc tcgatgaaat
     4501 tacgcttacc agcgagcagg atgcagttct gcgctatggt caggtggaag taccgctccc
     4561 gccgggagcc gacgttgagc gcacaacatg gggcatttca gtcaataaac ccaacgccgc
     4621 gaaacagcag gggcaattgc tggaaaaagc gcgtaatgaa atgggcgaac tggcctatat
     4681 ggtgccggtg aaagaactga cgggaacggt cactttccgc catttgctgc gcttctcgca
     4741 aaaagggcaa ttcgttctgc ctcctgctcg ttatgtgcgt tcctatgcac ctgcgcagca
     4801 aagtgttgcg gcagggagtg aatggaccgg gatgcaggtg aaataagtga actggcgcag
     4861 aatcgtctgg ctattggcgt tggttactct gccaacgctt gcggaggaga cgccgttaca
     4921 actggtgctg cgcggtgcgc agcacgatca actgtatcag ctgtcgtcat caggcgtgac
     4981 caaagtctca gcattacctg actcgctcac cacgccgtta gggagtttgt ggaaactgta
     5041 tgtttacgcc tggctggagg atacccatca accggagcag ccctaccagt gtcgcggaaa
     5101 ctccccggaa gaggtttatt gttgtcaggc tggggagagc ataacgcgcg ataccgcgct
     5161 ggtacgttcc tgtgggctct attttgcgcc gcagcggttg catattggcg cagatgtgtg
     5221 ggggcaatac tggcaacagc gtcaggcacc tgcgtggtta gcgtctctta caacgctgaa
     5281 gccagaaacc tcggtaacgg ttaaatcgct gctcgattca ttagcgacgt tacctgcgca
     5341 aaacaaagct caggaagtct tactggatgt ggtgctggac gaggcgaaaa tcggtgtcgc
     5401 atccatgctc ggtagtcggg tacgggtgaa aacctggagc tggtttgccg atgataaaca
     5461 ggaaatccgc cagggtggtt ttgctggctg gttaaccgat ggtacaccgc tgtgggttac
     5521 tggcagtggc accagcaaaa cggtactaac ccgttatgcg acggtgctta atcgggtttt
     5581 gccagtgccg acgcaggttg ccagtgggca atgtgtagag gtggagctgt ttgcccgcta
     5641 tccgctgaag aaaatcacag cggaaaagag tacgacggca gtaaaacccg gcgtacttaa
     5701 tgggcgctat cgtgtgacgt ttactaatgg taaccacatt acgtttgtca gccacggtga
     5761 gacgacctta ctgagcgaaa agggcaaact gaaattgcag tcgcatctcg atcgtgaaga
     5821 gtacgttgcc agagttctgg atcgcgaggc gaaaagtacg ccccctgagg cagcgaaagc
     5881 catgaccgtg gcgattcgca cttttttaca gcaaaacgcc aatcgggaag gcgattgcct
     5941 gaccatcccc gacagcagcg ccacgcagcg tgtttctgct tcgccagcga ccacgggggc
     6001 gcgaacgatg accgcctgga cgcaggatct catctatgca ggcgatccgg ttcattatca
     6061 tggcagtcga gctaccgaag ggacactttc ctggcggcaa gccacggccc aggcggggca
     6121 gggagagcgt tacgaccaga tactcgcgtt tgcttatccc gacaacagcc ttagccgctg
     6181 gggagcgcca cgctcaacct gccagttatt acccaaagcg aaagcctggc tggcgaaaaa
     6241 aatgccgcag tggcggcgta tattacaagg cgagacgggg tacaacgaac cagacgtgtt
     6301 tgcggtctgt cgtttagtct caggtttccc ctataccgat cggcagcaga aacggctgtt
     6361 tattcgtaac ttctttacgc ttcaggatcg gctggattta acccatgagt atctgcacct
     6421 ggcctttgat ggttatccca ccgggcttga tgagaactat atcgaaacgc tgacccgaca
     6481 attattaatg gactaatgct atgcgaaaga tttttcttcc gttgttactg gtggcacttt
     6541 cgcctgtcgc tcacagtgaa ggtgtgcagg aagtcgagat tgacgcaccg ctctctggct
     6601 ggcatccggc agagggcgaa gatgccagtt tttcacaatc gatcaattac ccggcgtcgt
     6661 ccgtcaacat ggcagatgat caaaatatct cagcgcagat ccgcggcaaa ataaagaatt
     6721 atgctgcggc gggtaaagtt cagcagggtc ggctggtggt caacggtgcc agtatgccgc
     6781 agagaattga atccgatggt tcatttgcac gcccttatat tttcactgaa ggcagcaaca
     6841 gcgtgcaggt catcagcccg gatgggcaaa gccggcaaaa aatgcagttt tactcaacgc
     6901 cgggtacagg aacgattcgt gcacgtttac ggctggttct ctcgtgggat acggacaata
     6961 ccgatctcga tctccatgtt gtcacgcccg atggcgaaca cgcctggtac ggtaacaccg
     7021 tgctgaaaaa cagtggtgca ctggatatgg atgtcacgac ggggtacgga cccgagattt
     7081 tcgccatgcc agcaccagtt cacgggcgct atcaggtgta tatcaactac tatggtgggc
     7141 gcagcgaaac ggaattgacg accgcccaac tgacgctgat caccgatgaa ggatcggtca
     7201 atgagaaaca ggaaacattt attgtaccga tgcgtaatgc tggcgagctg acgctggtga
     7261 aaagttttga ctggtgatcc atcccttcac acaacctggc tctctctttt ttcagataag
     7321 actgctgggc gatctgccga cgccaggata taaccattaa caagcgtcgg cagattggtc
     7381 atttcttatt tttcgtactg agaactgtcc atctactccg gctttcctgg ttaaggcaat
     7441 ggtttctaag cgatacgcat tattgcaatg gtgacagaac attgcggtaa cacgctttta
     7501 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
     7561 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
     7621 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
     7681 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
     7741 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
     7801 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
     7861 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
     7921 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctcaac
     7981 cgggtatttt taatcggcag tgataacctc tgcatggcca atttcggtct gcgtgatatg
     8041 ccagtggaac gcgataccgc gttgaaagcg ttgcatgaac gcatcaataa atatcgaaat
     8101 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
     8161 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
     8221 ggtgtcgagc agaccatccg gatggaaaac tttttcttac cgggtacgtt gccgatgggg
     8281 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
     8341 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
     8401 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
     8461 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
     8521 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
     8581 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
     8641 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
     8701 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
     8761 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
     8821 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
     8881 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
     8941 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
     9001 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
     9061 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
     9121 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
     9181 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
     9241 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
     9301 atgcgtttac agcaggtcat ctccaacctg ctgagtaacg ccataaaatt caccgatacc
     9361 ggctgtatag ttttgcatgt tcgcgctgat ggcgattatc tctctatccg tgttcgcgat
     9421 accggcgtgg gcattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
     9481 ggaacgggcg tacagcgtaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
     9541 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
     9601 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
     9661 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
     9721 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
     9781 gaagatgtgt taatcactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
     9841 accttctgtc gtcgccatat ttgtattccg ctggagaaag cgccagggga gtgggtacac
     9901 agtgtggctg ctccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
     9961 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataacgac
    10021 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    10081 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gcgtcgatgc gcttaatgta
    10141 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    10201 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    10261 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    10321 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    10381 aggaaatcgc gggaatcgta ggacggataa ggcgtttacg ccgcatccgg cattcagtgc
    10441 aaatgccaga taagacacta acgcgtctta tctggcctac aggtgattag tctttatctg
    10501 ccggacttaa cgtcacagaa gagagataat tcagcagggc gatatcgttc tcgacaccca
    10561 gcttcatcat cgcagatttc ttctggctac tgatggtttt aatactgcgg ttcagctttt
    10621 tagcgatctc ggtcaccagg aagccttccg caaacaggcg cagaacttca ctctcttttg
    10681 gcgagagacg cttgtcaccg taaccaccag cactgatttt ttccaacagg cgagaaacgc
    10741 tttccggggt aaatttcttc cctttctgca gcgcggcgag agctttcggc agatcggtcg
    10801 gtgcaccttg tttcagcacg atcccttcga tatccagatc caataccgca ctaagaatcg
    10861 ccgggttgtt gttcatggtc agaacaataa tcgacaggct tgggaaatgg cgcttgatgt
    10921 acttgattaa ggtaatgcca tcgccgtact tatcgccagg catggagaga tcggtaatca
    10981 acacatgcgc atccagtttc ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc
    11041 cgacaacatt cacccactca atttgctcaa gtgatttgcg aataccgaac aagactatcg
    11101 gatggtcatc ggcaataatt acgttcatat tgttcatgta ataggctacc ttgctacagc
    11161 aagctcttga cataactgtc aatgtcgctg atgtattttt ctattcctgg aacatccttc
    11221 tcacgaatca gatgttccag cgtttcacat aactgcttgc cgggtaccag atttagcatg
    11281 gcaaatacgc ctttaagacg atgagccgtt tgggctaacg cagcaaagtc actggttgct
    11341 gcttcagtat acagcctctt aacatcatcc ggtactgtgt ctacaaagag cgcataatag
    11401 ccgctggcat ggagttgcgc attttcatct ccgcccagag gcgattctgt cacctcttcc
    11461 tgcgccagtt gcacttcaat taattgtaag accgcttcct gcatagcgtt gctcatattg
    11521 aagttgacgc acaattgacc aggcccaatt tcccgtacgc cagactcatc atcgcttaaa
    11581 agcaagccag aggcagtaag attagacgga ttatccgtta aaaagatatc ataatcttga
    11641 ctaattaatc tttcatcggg tgtgatacag gttgcacccc aattttctaa ctggcgagtg
    11701 acaatattcc gaatttctgc cgaagtaaca tccaccatta cgcagacatc atccagtaaa
    11761 cgctcttctt cctcttcaac ttccgggtca gcggcgagca ttttgatatg cacagagtag
    11821 cgtgtaccaa gcccatcccg cgttttgatg tttaaatgac cgcccagttt acgcgccagt
    11881 tgatcgctca gccagaatgc cagcgggtcc gccttgccat agcgatcgtt ttgggtctgg
    11941 ttgataaacg ggaagtgcaa attatccatt tcatgaatac ttacgccttc tccggtgtcc
    12001 agaatgcgga atgtcaggcg gtcttcagag gactcatcct gatcaacctc aagggtgatt
    12061 tttcccaatt gcgttgaggt cacggcatat tgcatcagta gcagcaaaat acgtcgtaag
    12121 gcatcgcgat cgccgcggcg catatcgtgt gctttcagat gattgttaat cagcagttgc
    12181 agacctttac gcttgatggc aggcaacact gaaggcacaa cttcatcaat taaatcctgc
    12241 acggagaaca gcaccgtctc acttttccag ctatcatccg caagcatgtt cgctaactgt
    12301 atttcatcaa ccagccgcac cagcacatct gcctgattcg ccagttgttt gctttccggg
    12361 gcgttgagtt tagccgcgct ctccgccagg gactgtgcag gttctttcag cgcatcgcca
    12421 atgtttttca taaaggtcat ccggccctgc tggtttttct catacagacg ctgcgcctgc
    12481 ttgagtttct tgtttaccag cacttcgcga tcctgatcgc gaataatgaa aatttgtgtg
    12541 cgcggcgcga cctggctgcg gaacatgcgg atctcataca gctcgttatt gatcgtcgcc
    12601 tgaataatcc cctgatgctg ttccgccatg gtggtgatgt tttgcagatt caaatgcggc
    12661 agcaaatgat cggcaatttt gttacttatg acagtgcggt tcgattcctg atcgtgaacc
    12721 agcaggccga gcggcagcag tgagactatc tcttcattaa ttgcccgtaa aatgcgcaat
    12781 tcgttattga ccgccgtgct gggtagactt tctgtactgc ggctggagaa atggcggaat
    12841 gtggtatagc caaataacgc cagcgccagc aaaccgatgt tcagcagcag tggcagcaga
    12901 atgttttgca acgtatccag caataaggtg ccataaggaa cctgccagac caggcgcata
    12961 tcggtagagt tgagtgccga ggagatttca atcttcgtac tgttaaagtg gatactgacg
    13021 ctatccgtcc cttctttctc attatcattg tttcccgttg ccgtcgcgtc tggctcaagg
    13081 cggaaactgt ccagcggcat acccggtggg atcaaatcat taatcggcag atcaaaagcc
    13141 acgaccgttg ccagatgtcc tggctggtta aaggtagtac gcaaggtaaa gtaatgaccg
    13201 ttctgccagg ccaggcggcg catgttagaa aagctttcgc gttcatcgag ggcgttggcc
    13261 tgttgcaaca tctctgcacg acgtgaatca acaatgtcgc tgacggtcga ttctttaaat
    13321 ccggaggtga gatctttaag gggtagggtt gagatcagca ccagactgtt atcctgacca
    13381 ttcaggtaat acatcgacca cggtacattt tctgcgcccc acaatgtatc cagataagtg
    13441 gacatccgct gagtcatctc aagcgttgag ttgtcgtgag agccaaagat taacgcttcc
    13501 gttttgcggc gtggtttctc cagatagtag acatcctgtt tcaggcgcgt ctcttgtaaa
    13561 ccttcgccgg aggaggggga ggtcgtcgcg gcaatgttgt cgtagatctg ccaggtcacg
    13621 taacgccagg tatcgacgcg cttttggata gcgtgggtaa tgtcgacaat ctggtaactt
    13681 ttatctttca gccatgcgtt aacggcgctt tgtaccatta cacccatcgt caccagtaac
    13741 acaatgatca acagtaaaaa gaagcgggta atgctccccg gtaggagtga aaagcgggtc
    13801 gtggccgttg tctctttctg acgcattcgt gtttatgacc tgttaaaact tcgcgaagtg
    13861 atagggcagt ataaagggta cagtgtgaaa ttccagactc ttacctgatt gatgattctc
    13921 tattctcccg tgatatttat catcctgcat tgggggaaat agatgtacaa gcgccatttt
    13981 gctgtacaaa taacctacaa aaagcccaac gaaaataatt gcaaataaac aaagattgct
    14041 ggaaattatg ctgatgttaa ttatttgtga aatagttaac aagcgttata gtttttctgt
    14101 ggtagcaaag aataatgaaa agtgtgtaaa gaagggtaaa aaaaaccgaa tgcgaggcat
    14161 ccggttgaaa taggggtaaa cagacattca gaaatgaatg acggtaataa ataaagttaa
    14221 tgatgatagc gggagttatt ctagttgcga gtgaaggttt tgttttgaca ttcagtgctg
    14281 tcaaatactt aagaataagt tattgatttt aaccttgaat tattattgct tgatgttagg
    14341 tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac attttgaaac
    14401 atctatagcg ataaatgata catcttaaaa gttttagtat catattcgtg ttggattatt
    14461 ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt
    14521 ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa gttaaagtac
    14581 tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct gaagtttaca
    14641 acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac tatttctctg
    14701 acaacaaaga tgtagatggc gaccagacct acatgcgtct tggcttcaaa ggtgaaactc
    14761 aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag ggcaacagcg
    14821 ctgaaaacga aaacaactcc tggacccgtg tggcattcgc aggtctgaaa ttccaggatg
    14881 taggttcttt cgactacggt cgtaactacg gcgttgttta cgacgtaact tcctggaccg
    14941 acgtactgcc agaattcggt ggcgacactt acggttctga caacttcatg cagcagcgtg
    15001 gtaacggttt cgcgacctac cgtaacactg acttcttcgg tctggttgac ggcctgaact
    15061 ttgctgttca gtaccagggc aaaaacggta gcgtaagcgg cgaaggcatg actaacaacg
    15121 gtcgtgaagc actgcgtcag aacggcgacg gcgttggcgg atctatcact tatgattacg
    15181 aaggcttcgg tatcggtgct gcagtttcca gctccaaacg tactgatgat cagaacagcc
    15241 cgctgtacat cggtaacggc gaccgtgctg aaacctacac tggtggtctg aaatacgacg
    15301 ctaacaacat ctacctggct gctcagtaca cccagaccta caacgcaact cgcgtaggtt
    15361 ccctgggttg ggcgaacaaa gcacagaact tcgaagctgt tgctcagtac cagttcgact
    15421 ttggtctgcg tccgtccctg gcttacctgc agtctaaagg taaaaacctg ggtcgtggct
    15481 acgacgacga agatatcctg aaatatgttg atgttggtgc gacctactac ttcaacaaaa
    15541 acatgtccac ctatgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg
    15601 acgctggcat caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc
    15661 gattgatatc gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaacac
    15721 agttttttgg tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct
    15781 ttacccgcgt ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac
    15841 aaaccgccaa aacccacgct actgaagtta ccgttcttga aggcaaaact atgggtacct
    15901 tctggcgtgc cagcatcccg ggcattgacg ccaaacgcag tgccgaactt aaagaaaaga
    15961 ttcagaccca gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc
    16021 tgatgcgctt taacgattcg caaagtttat cgccgtggcc ggtaagtgaa gcgatggccg
    16081 atatcgtcac cacctcgctg cgcattggcg caaggaccga tggtgcgatg gatataaccg
    16141 tcgggccgct ggtgaatctg tggggctttg gcccggaaca acagccggtt caaattccga
    16201 gccaggaaca gatcgatgcg atgaaagcca aaaccggctt acagcacctg acggtcatta
    16261 atcagtcgca tcagcaatat ctgcaaaaag acctaccgga tttatatgtc gatctctcca
    16321 ccgtcggtga aggttatgcg gcggatcacc tggcacgctt gatggagcag gaagggattt
    16381 cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg tggtatgaac ggtgaaggcc
    16441 tgccgtggcg ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt caggctgtgg
    16501 tggatatcaa cggtcatggt attagcacct ctggcagtta ccgtaactat tacgaactgg
    16561 acggcaaacg tctttctcat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc
    16621 tggtatccgt gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct
    16681 tgatggtact cgggccggag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt
    16741 atatgatcac caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct
    16801 tccttgtcag cgaaaaaaat taaagcgcaa gattgctggt ttttgcgtga tggtgaccgg
    16861 gcagactaaa ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact
    16921 gacgatcaac gctggcaatc tgtcttagcc cgcgacccga atgccgacag cgaattcgtt
    16981 ttcgccgtgc gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgccttg
    17041 cgggaaaatg tctccttcta cgcaaatgcc tgcgaggcac tcgccgccgg atttcgcccc
    17101 tgcaaacgct gccaacctga taaagccaat gcccagcaac atcggttgga taaaatcacc
    17161 cacgcgtgtc gactgctgga acaggaaacg cctgtaacgc tggaagcctt agccgaccag
    17221 gtggcgatga gtccatttca tctacatcgg ttgtttaaag cgactaccgg aatgacgcct
    17281 aaagcctggc aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag
    17341 agcgtgacga cgtctattct taacgccgga ttccccgaca gcagcagtta ctatcgcaaa
    17401 gctgacgaaa cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg
    17461 gtgcgttacg cgctggctga ttgtgagctg ggtcgttgcc tggtggcaga aagcgagcgg
    17521 gggatttgcg cgatattgct gggcgatgat gacgcgacac taatcagcga gttgcagcag
    17581 atgtttcccg ctgccgacaa cgcgcctgcc gatctgatgt ttcagcaaca tgtgcgtgaa
    17641 gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt taccgctgga cattcgcggc
    17701 actgcttttc agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgtc
    17761 agttatcagc aactggctaa cgccatcggc aaaccgaaag cggtacgggc cgttgccagc
    17821 gcctgtgccg ccaacaagct ggctatcata ataccctgtc atcgggtggt ccgtggtgat
    17881 ggcacacttt ccggttaccg ctggggcgtg tcgcgtaaag cgcaactgct gcgccgcgaa
    17941 gctgaaaatg aggagaggta atgttggatc tgtttgccga tgctgaaccg tggcaagagc
    18001 cactggcggc tggagcggta attttgcacc gttttgcttt taacgctgcg gagcaactga
    18061 tccgcgatat taatgacgtt gccagccagt cgccgtttcg ccagatggtc acccccgggg
    18121 gatataccat gtcggtggcg atgaccaact gtgggcatct gggctggacg acccatcggc
    18181 aaggttatct ttattcgccc attgatccgc aaacaaataa accgtggccc gccatgccac
    18241 aaagctttca tgatttatgt caacgtgcgg ctacggcggc gggctatccg gatttccagc
    18301 cagatgcttg tcttatcaac cgctacgctc ctggcgcgaa actgtcgctg catcaggata
    18361 aagacgaacc ggatctgcgc gcgccgattg tttctgtttc tcttggctta cccgcgattt
    18421 ttcaatttgg tggcctgaaa cgtaatgatc cgctcaaacg tttgttgttg gaacatggcg
    18481 atgtggtggt atggggcggt gaatcgcggc tgttttatca cggcattcaa ccgctgaaag
    18541 cggggtttca tccactcacc ggcgactgcc gctacaacct gacattccgt caggcaggta
    18601 aaaaagaata aaaataagaa ttattattgc tgtgcgcgcg aagatgttta aactgcgggc
    18661 tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg cggcagtatc
    18721 gctggccatt tatcagtgtg atggcgctaa gcctcgccag tgcggcatta ggcattggct
    18781 taattgcttt tatcaatcag cgccttatcg aaacggcgga taccagtctg ctggtattgc
    18841 cggagtttct gggattattg ctgctgttga tggcagtcac tctcggatcg caactggcgc
    18901 tcaccacttt ggggcatcac ttcgtttacc gactgcgtag tgaatttatc aagcggattc
    18961 tggataccaa cgtcgagcgc attgaacaac tcggtagcgc ctcgttgctg gcgggattaa
    19021 ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct tccggaactg gtgcagggga
    19081 tcattctcac tatcggttcc gcggcgtatc tgtggatgct gtcgggcaaa atgttgctgg
    19141 taacggctat ctggatggcg atcaccatct ggggtggttt tgtactggtg gcgcgggtgt
    19201 acaaacatat ggcgaccctg cgtgaaaccg aagacaggct gtatacggat tttcaaactg
    19261 tactcgaagg gcgcaaagag ctgactctga accgggaacg cgccgagtat gtgtttaaca
    19321 acctctacat tcctgatgcg caagagtatc gccaccatat tatccgcgca gacaccttcc
    19381 atcttagcgc cgtgaactgg tcaaacatca tgatgttggg cgcaatcggc ctggtgttct
    19441 ggatggcgaa cagtctcggt tgggctgata ccaacgttgc cgcgacctat tcgttgacgc
    19501 ttttattcct gcgtacgccg ctgctttcgg cggttggcgc attgccgacg ctgctgacgg
    19561 cgcaggtggc gtttaacaag ctgaacaaat tcgcgctcgc gcctttcaaa gcagagtttc
    19621 cgcgcccgca ggcgtttccc aactggcaaa cgctggagct gcgtaacgtg acgtttgctt
    19681 atcaggataa cgcgttttcc gttggtccga ttaatctcac catcaaacgt ggcgagctgc
    19741 tgtttctgat tggcggcaac ggtagcggaa aatcgacgct ggcgatgttg ttgacgggct
    19801 tgtatcagcc acaaagcggc gaaatcttgc tggatggcaa acctgtcagc ggcgaacaac
    19861 cggaagatta tcgcaaactg ttttcggcag tgtttaccga tgtctggctg tttgatcaac
    19921 tgctggggcc ggagggtaaa cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc
    19981 tgaaaatggc tcataagctt gagttaagca acgggcgtat tgttaacctg aagttatcaa
    20041 aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc gatattatcc
    20101 tgctggatga atgggcggcg gatcaggatc cacacttccg tcgtgagttt tatcaggtgt
    20161 tgctgccgct gatgcaggag atgggtcaaa ctattttcgc catcagtcat gatgatcatt
    20221 actttatcca cgccgaccgc ctgctggaaa tgcgcaacgg gcaacttagc gagctgacgg
    20281 gcgaagagcg cgatgccgct tcgcgtgatg ccgttgcccg gacggcataa tattacgacg
    20341 ctgacctggc ggactgctgc cgtcaggtca atatgctaaa aatcccttca tttctaataa
    20401 ccctataatt aatcaacgaa attataatgt ttctaaaatt agaatataat ttataaacat
    20461 tatttaaatg ttgttactta agtgttaccg ttgatgccgc gcaaatctca cctgcaataa
    20521 agcgactaaa agtaaggcat taacaagatg aaaaaagtga ctgccatgct cttctcgatg
    20581 gccgtggggc ttaatgccgt ttcgatggcg gcaaaagcga aagcgtccga ggagcaggaa
    20641 actgatgtac tgttgattgg cggcggcatt atgagcgcca cgttggggac ctatttacgc
    20701 gagctggagc ctgaatggtc gatgaccatg gtggagcgcc tggagggtgt cgcgcaggag
    20761 agttcgaacg gctggaataa cgccggaacc gggcattctg cactgatgga actgaactac
    20821 accccgcaaa acgccgatgg cagcatcagt attgaaaaag cagtcgccat taacgaagca
    20881 tttcagattt cccgccagtt ctgggcgcac caggttgagc gcggcgtgct gcgtactccg
    20941 cgttcattta tcaataccgt tccgcatatg agctttgtct ggggcgagga taacgttaat
    21001 ttcctgcgcg cccgttacgc cgcgttgcaa caaagctcgc tgtttcgcgg tatgcgttac
    21061 tctgaagatc acgcgcagat caaagagtgg gcaccgttag tgatggaagg gcgcgatcca
    21121 caacagaaag tagcggcaac acgcacagaa attggcactg atgtgaacta tggcgagatc
    21181 acccgccagt taattgcttc cttacagaag aaatctaact tctcgctgca actcagcagc
    21241 gaagtccgcg ccctaaagcg taatgacgat aacacctgga ccgttaccgt tgccgatctg
    21301 aaaaacggca ctgcacagaa catccgcgcc aaatttgtct ttatcggcgc gggcggtgcg
    21361 gcgctgaagc tattacagga atcggggatt ccggaagcga aagactacgc cggtttcccg
    21421 gtgggcggac agttccttgt ttcggaaaac ccggacgtgg ttaatcacca tctggcgaag
    21481 gtttacggta aagcatccgt tggcgcacca ccgatgtcgg ttccgcatat cgatacccgc
    21541 gttctggacg gtaaacgcgt agtgctgttt gggccatttg ccaccttctc aaccaaattc
    21601 ctcaaaaacg gttcattgtg ggatctgatg agttccacca ccacctctaa cgtgatgccg
    21661 atgatgcacg tcgggctgga taatttcgat ctggtgaaat atctggtgag tcaggtgatg
    21721 ttgagtgaag aggatcgttt tgaagcgttg aaagagtact atccgcaggc aaaaaaagag
    21781 gactggcgtt tgtggcaagc ggggcagcgc gtgcagatta tcaagcgtga tgccgagaaa
    21841 ggtggcgtac tgcgtctggg tactgaagtg gtcagtgacc agcaagggac cattgccgcg
    21901 ctgctggggg catcgccagg ggcgtcaacc gccgcgccga ttatgttgga tctgctggaa
    21961 aaagtatttg gcgatcgcgt ttccagcccg caatggcagg ctacgttgaa agcgatcgtt
    22021 ccgtcttatg gacgcaagct gaacggtgat gtagcggcaa cagaacgcga gttgcagtac
    22081 accagcgaag tgctggggct gaaatacgac aagccgcagg cagcagatag tacgccaaaa
    22141 ccgcagttga aaccgcaacc cgttcaaaaa gaagtggcgg atattgcgtt gtaatgatgt
    22201 gccacatccg gcatggtatg ccggatgtgg cgtatgctga taagacgcgc cagcgtcgca
    22261 tcaggcaccg gctcgggcgt tagatgtcgg atgcggcgtg accgccttat tcggcctacg
    22321 gggtgccgca cttcacagca ttttagcgaa ctaccgcgtt gtcaattttc tcttccgcct
    22381 tccagacgcg gtacttcaca tctacattgt ctggcgtata caccacgatc ggcagcttgc
    22441 tgttgtaacg cagcattcca gcatcgccca gatacgcggt gacaaatttc ttctctttct
    22501 tgccatccgg gcaggccatc atcgtggaaa ccggcgagct aactttatca aagacataat
    22561 aatcatagcc ccagccttcc agcgttttgc tttccagctt cccgccgaga cgatgcaaat
    22621 tgcaatcgac ttccagcgtc tgaccgatta acagttctac tttcagggta gattcatctt
    22681 cttgcggggt taactgaatc acctgacgct tcatcccttt ttcagcttgt ggataaggcg
    22741 cgattttttc cagtggctgg acgctttctg ccgcccaggc ggaagtggta gcgaaagcgg
    22801 caaacaatac tgcaggtaga atggtcttca tttttttgct ttccttcttc aatttatagg
    22861 ttttcaccga tgatgtcgct gaaggttaac atattcatca gatttattaa tttgctttat
    22921 gaataactta a
//
LOCUS       DAZFJF010000075        22695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFJF010000075 DAZFJF010000000
VERSION     DAZFJF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.075"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..525
                     /locus_tag="ACHKAU_003114"
     CDS             <1..525
                     /locus_tag="ACHKAU_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO5532125.1"
                     /translation="GDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVN
                     HWLLNGGGWFFNLDYDKEILAKALAHKAGELPLIIELVSKDKKYVICHADYPCDEYEF
                     GKPVDHQQLIWNRERISNSQDGIVKEIKGADMFIFGHTPAVKPLKFANQMYIDTGAVF
                     CGNLTLIHVQGEGA"
     gene            522..1145
                     /locus_tag="ACHKAU_003115"
     CDS             522..1145
                     /locus_tag="ACHKAU_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5532126.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            2218..2541
                     /locus_tag="ACHKAU_003116"
     CDS             2218..2541
                     /locus_tag="ACHKAU_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO5532127.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2525..3001
                     /locus_tag="ACHKAU_003117"
     CDS             2525..3001
                     /locus_tag="ACHKAU_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO5532128.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            2998..3435
                     /locus_tag="ACHKAU_003118"
     CDS             2998..3435
                     /locus_tag="ACHKAU_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5532129.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            3641..4159
                     /locus_tag="ACHKAU_003119"
     CDS             3641..4159
                     /locus_tag="ACHKAU_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5532130.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            4443..4814
                     /locus_tag="ACHKAU_003120"
     CDS             4443..4814
                     /locus_tag="ACHKAU_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO5532131.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            4918..5160
                     /locus_tag="ACHKAU_003121"
     CDS             4918..5160
                     /locus_tag="ACHKAU_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO5532132.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            5240..5665
                     /locus_tag="ACHKAU_003122"
     CDS             5240..5665
                     /locus_tag="ACHKAU_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFO5532133.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            5662..7074
                     /locus_tag="ACHKAU_003123"
     CDS             5662..7074
                     /locus_tag="ACHKAU_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFO5532134.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            7077..9203
                     /locus_tag="ACHKAU_003124"
     CDS             7077..9203
                     /locus_tag="ACHKAU_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO5532135.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDPMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYRKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVVVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            9217..10101
                     /locus_tag="ACHKAU_003125"
     CDS             9217..10101
                     /locus_tag="ACHKAU_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFO5532136.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDNDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKHAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            10113..11384
                     /locus_tag="ACHKAU_003126"
     CDS             10113..11384
                     /locus_tag="ACHKAU_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO5532137.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            11427..11612
                     /locus_tag="ACHKAU_003127"
     CDS             11427..11612
                     /locus_tag="ACHKAU_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532138.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            11587..12069
                     /locus_tag="ACHKAU_003128"
     CDS             11587..12069
                     /locus_tag="ACHKAU_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO5532139.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            12078..13496
                     /locus_tag="ACHKAU_003129"
     CDS             12078..13496
                     /locus_tag="ACHKAU_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO5532140.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            13496..14344
                     /locus_tag="ACHKAU_003130"
     CDS             13496..14344
                     /locus_tag="ACHKAU_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFO5532141.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            14344..14799
                     /locus_tag="ACHKAU_003131"
     CDS             14344..14799
                     /locus_tag="ACHKAU_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO5532142.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            14802..15494
                     /locus_tag="ACHKAU_003132"
     CDS             14802..15494
                     /locus_tag="ACHKAU_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO5532143.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            15504..16910
                     /locus_tag="ACHKAU_003133"
     CDS             15504..16910
                     /locus_tag="ACHKAU_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO5532144.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIDRS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            16910..18754
                     /locus_tag="ACHKAU_003134"
     CDS             16910..18754
                     /locus_tag="ACHKAU_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO5532145.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(18769..19254)
                     /locus_tag="ACHKAU_003135"
     CDS             complement(18769..19254)
                     /locus_tag="ACHKAU_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532146.1"
                     /translation="MKKSLLIIPFLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(19325..19591)
                     /locus_tag="ACHKAU_003136"
     CDS             complement(19325..19591)
                     /locus_tag="ACHKAU_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5532147.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            19713..21836
                     /locus_tag="ACHKAU_003137"
     CDS             19713..21836
                     /locus_tag="ACHKAU_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFO5532148.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIINGAGQTDSTAPLAGNGKWSYVSCGVYIYETDTAINV
                     GIRNYGSGNVLINSPSLCAYGMPSNALQVEKTTFYLSSVPTSGFWDIGERVINSAPAS
                     GQPKAWTCNIPGGPGTYSFLSEGNF"
     gene            21907..>22695
                     /locus_tag="ACHKAU_003138"
     CDS             21907..>22695
                     /locus_tag="ACHKAU_003138"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5532149.1"
                     /translation="MERKTNKLDSIQVLRGIAVLLVIAFHFRVYLNGVYAQKDLGDIL
                     FGIGEVGVDIFFVISGFIITYSSMNKAKNRPMVFAAKRFFRLYPVYFIILTLAIYLNY
                     EAIYTSSQIIKSYLLIPIDYNFIGPWSGYSIIIPAWTLTYEIWFYVVFFFSLCISHKY
                     RTAIASFLLIIICVLGQLYFHGDVFLNPIAQPPSGDGIIRNLVFVSNPIIYDFILGMI
                     IAEAYIRLPSRLINKMPVVQFLNITLAIALLFLCLGLRNGPGIVA"
BASE COUNT         6438 a         5115 c         5596 g         5546 t
ORIGIN      
        1 ggcgatttgg tcgatcgcgg tacagagaac gtcgaatgtc tggaattaat cacattcccc
       61 tggttcagag ctgtacgtgg aaaccatgag caaatgatga ttgatggctt atcagagcgt
      121 ggaaacgtca atcactggct gcttaatggc ggtggctggt tctttaatct cgattacgac
      181 aaagaaattc tggctaaagc tcttgcccat aaagcaggtg aacttccgtt aatcatcgaa
      241 ctggtgagca aagataaaaa atatgtcatc tgccacgccg attatccttg tgacgaatac
      301 gagtttggaa agccagttga tcatcagcag ttaatttgga accgcgaacg aatcagcaac
      361 tcacaagacg ggatcgtgaa agaaatcaaa ggagcggaca tgttcatctt tggtcatacg
      421 ccagcagtga aaccactcaa gtttgccaac cagatgtata tcgataccgg cgcagtgttc
      481 tgcggaaacc tcacattgat tcatgtacag ggagaaggcg catgagactc gaaagcgtag
      541 ctaaatttca ttcgccaaaa agcccgatga tgagcgactc accacgggct acggcttctg
      601 actctctttc cggtactgat gtgatggctg ctatggggat ggcgcaatca caagccggat
      661 tcggaatggc tgcattctgc ggtaagcatg aactcagcca gaacgacaaa caaaaggcta
      721 tcaactatct gatgcaattt gcacacaagg tatcggggaa ataccgtggc gtggcaaagc
      781 ttgaaggaaa tactaaggca aaggtactgc aagtgctcgc aacattcgct tatgcggatt
      841 attgccgtag tgccgcgacg ccgggcgcaa gatgcagaga ttgtcacggt acaggccgtg
      901 cggttgatat agcaaaaaca gagcagtggg ggagagttgt tgagaaagag tgcggaagat
      961 gcaaaggcgt cggctattca aggatgccag caagtgccgc atatcgcgct gtaacgatgc
     1021 taatcccaaa ccttactcaa cccacctggt cacgcactgt taagccgctg tatgacgctc
     1081 tggtggtgca atgccacaag gaagagtcaa tcgcagacaa cattttgaac gcggtcacac
     1141 gttagcagca tgattgccac ggatggcaac atattaacgg catgatattg acttattgaa
     1201 taaagttggg taaatttgac ccaacgatgg gttaattcgc tcgttgtggt attgagataa
     1261 aaagaggcgg cgcttactac cgattccgcc cagttggtca cttcgacgta tcgtctggaa
     1321 ctccaaccat cgcaggctga gaggtctgca aaatgcaatc ccgaaacagt tcgcaggtaa
     1381 tagttagagc ctgcataacg gtttcgggat tttttatatc tgtgtaacag gtaagagcat
     1441 tctcccttat ggggcttggc ttaaatgcac cgagtgctct tatcgttgtg gcagcacaac
     1501 gatagttttc gtcagagttg gcgactttgc ggttttttag aaactgacca caaagataaa
     1561 tgcaaacgat gatgttgttc tgatggcggc gtaatagcct gtaagtcagc aaggtcttcc
     1621 gactccttgt aaacaaattc ggcgcactgg cccggtgtga ttaataatgg gcacacaaca
     1681 ggtaagagca ttaaagaact ggcaaagagc ttaacggtct gcgaaagcat ttcttagtgg
     1741 cacaactggc cggtacaact gagtgctctt tccggtgtgg tgaatgcgca ggctgatgcg
     1801 cgcaggagag cttcggaaga acaaggtgcc tgtatacaag ccggagatca gcgccggcca
     1861 ccacagccaa atccacccag agcaaaaccg ttgttcatcc ttaccattca ctcagtattt
     1921 tgggctacac catcagccca ttttttaaag cgtacttcca ccaagaacca gacctaacca
     1981 actcattgct gacactctgt ggatacggtt gtctagtacg ctttaaaaaa gaaaacccag
     2041 catcaatggc tgggcttcgt gatatgagcg gcatgtattg ttggcgcaat ccacgcctga
     2101 tttgctcatg aatgcggtca cgaacaaacc cgttacaaat caaccgtaac ccggatttgt
     2161 tcaagcgacc atatccataa ttcctaattt gaacagatcc ccttctgggg gtaagacatg
     2221 aagatgccag aaaaacatga cctgttagcc gctattctcg cggcaaagga acaaggcatc
     2281 ggggcaatcc ttgcgtttgc aatggcgtac cttcgcggca gatataatgg cggtgcgttt
     2341 acaaaaacag taatcgacgc aacgatgtgt gccattatcg cctggttcat tcgtgacctt
     2401 ctcgacttcg ccggactaag tagcaatctc gcttatataa cgagcgtgtt catcggctac
     2461 atcggtactg actcgattgg ttcgcttatc aaacgcttcg ctgctaaaaa agccggagta
     2521 gaagatggtg gaaatcaata atcaacgcaa ggcgttcctc gatatgctgg cgtggtcaga
     2581 gggaacagat aatggacggc agaaaaccag aaatcatggt tatgacgtca ttgttggcgg
     2641 agagctattc actgattact ccgatcatcc tcgcaaactt gtcacgctaa acccaaaact
     2701 caaatcaaca gccgccggac gttaccagct tctttcccgt tggtgggatg cctatcgtaa
     2761 gcagcttggc ctgaaagact tctctcccaa aagccaggac gctgttgcgc tgcagcagat
     2821 taaagagcgt ggcgctttgc cgatgattga tcgcggtgat attcgtcagg caatcgaccg
     2881 ttgcagcaat atctgggctt cactgccggg cgctggttat ggtcagttcg agcataaggc
     2941 tgacagcctg attgcaaaat tcaaagaagc gggcggaacg gtcagagaga ttgaggtatg
     3001 agcagagtca ccgcgattat ctccgctctg gttatctgca tcatcgtctg cctgtcatgg
     3061 gctgttaatc attaccgtga taacgccatg acctacaaag agcagcgcga taaagccaca
     3121 tccatcatcg ctgatatgca gaagcgtcaa cgtgatgtag cagaactcga cgccagatac
     3181 acaaaggagc ttgctgatgc taacgctact atcgaaagcc ttcgtgctga tgtttctgct
     3241 ggtcgtaagc gcctgcaagt cgccgccacc tgtgcaaagt caacgaccgg agccagcagc
     3301 atgggcgatg gagaaagccc aagacttaca gcagatgctg aactcaatta ttaccgtctc
     3361 cgaagtggaa tcgacaggat aaccgcgcag gttaactacc tgcaggagta catcaggact
     3421 cagtgcctga aataattttt ttgcaaatca caaagtccat ttaatgagcc tcgcgatgcg
     3481 gggctttttt tacatctgaa tttcacagcg catctcacgc gcatattaca tcacccgagc
     3541 ctttcagaaa gttgagcctg agaactgccg tatatggtgg cgaccatctc ggggcggctt
     3601 ttctgtgaga caggctcact ttctaaaagg taaagacgct atgaatcatc aattggctaa
     3661 tctcgatttc cgggacatgg tggttgtttc tggtgatcgc gtgatcacaa cctcccgcaa
     3721 ggtagcagct tacttcgaca agcagcatca ccacatcatt cagaaaatcg aaaagctaga
     3781 ctgttcggat gaatttctaa ccagcaactt ttcgcgggtt acctatgaac acaagggtaa
     3841 tcagtatgtt gaatatgaaa tttccaaaga cggtgcgatg tacatcatca tgtcgtttac
     3901 cggcaaaaaa gctgccgcca tcaaagaggc gtttatcaaa gcatttaatt ggatgcgtga
     3961 caggctgatg gagatggctc actcatacca aagagagcac aacgagttaa tgctggagtt
     4021 catgaaggaa aaggatgttg ccagtatgtc aggacgcttg ctgaaccgct ggggcaggat
     4081 caaaaaaccg caactcatag caagaatcaa aaggcttgag cagcaggcgc aaatatcgat
     4141 ccccggactg ccaaagtgac cattccaaag cccatctacg ggtgggcttg ataatgaaac
     4201 cgtgatttac atccccacaa tccgggtatg taaaagatag ttcaggcgag aacagattta
     4261 actaaatctg tgcaccacca gttacggcag taccgcgaaa caacccaagc cagtaagtgg
     4321 ggaaataaca ctggcagcca ctgaaagatg aacctccagc cttatggcaa aaaagattct
     4381 ttgtggtggc gaactgatgg aaagacatcc taatcaagca accactctac agggtcataa
     4441 ttatgaacga ccagcaaatc gaaaaagaaa tcgttgagaa aggcaaaacg gcaccgcgag
     4501 ttacgccaga ccatatcgaa ggaattattg ctcaggaggc atatttcaca gcagaggatg
     4561 gtgcctttgg caaagccata aaagcgaaac atactggcgg agaggtaaac taccagtcgc
     4621 acgaatcact ttctctgctg acgttctgcg tcctggtgct gcgcaacggc ttcaccgtca
     4681 ccggagagag tgcctgtgca agtcaggaaa attttgatgc agaaattggt cggaagattg
     4741 cccggcagaa tgctgtaaac aaaatctgga tgctcgaagg ttacttgctg aagcagaagc
     4801 taagcgaaca gtagttatta caaaagccat tccttactga gtggctttga taatggctta
     4861 taccctacac gggataactt aactgatatc ccttttaacg gataaacgga gccaacaatg
     4921 gcagagatta ttcccatgac tgaagaacag aaattccagt tagagattta caaactggtc
     4981 atgaaccaga acgcagccgc ggaggaagca tttcagttca ttggcactga cgagctgaag
     5041 cttgagctat tcaaaattca cttccagtca ggtggcgcta attcagatat cacgacccgc
     5101 acaatcgaag cggtgcgtaa atcgaaggaa gcgttagacc tgttcactac cggagcgtaa
     5161 acatggcaac tcaaggtttc gacaacccat ccaaattccg cgatgaatgg gataagcaag
     5221 cagaagggaa ataatcaata tggcgactga gaaaaagaaa ggtggtcgcc cctctgatta
     5281 tatgccggag gtggctaatg acatttgcgc attgctttcc tccggtgaga gtctgcgcaa
     5341 agtttgcgaa cgcccaggaa tgccgagcaa aacatcagtt tttcgctggc tggctgaaca
     5401 tcaggagttt cgtgaccagt acgcgaaggc aacagagact cgggccgact ctattttcga
     5461 agagatattc gaaattgctg acgacgtaat ccctgatgcc gccgaggtgg caaaggcaag
     5521 acttcgcgtt gatacccgca aatgggcgct ggcccgaatg aatccccgta agtatggcga
     5581 caaggtaact aacgagcttg tcggcaaaga cggcggcgca atccagattg aaacatcacc
     5641 gatgagcact ctattcggaa aatgacctcg attaatccta tctttgaacc gttcattgag
     5701 gcgcatcgct acaaagtcgc caaaggcggt cgaggtagcg gtaagtcatg ggcaattgcg
     5761 aggcttcttg ttgaagcggc gcgtcggcag cctgtgcgta ttctttgcgc tcgtgagctg
     5821 caaaacagta tcagcgattc ggtaatcagg ttgcttgaag acaccatcga gcgtgaaggg
     5881 tattcggctg agtttgaaat tcagcgttca atgattcgtc atctcggaac gaatgctgaa
     5941 ttcatgttct acggcatcaa aaacaacccg acgaagatta aatcgctcga aggcattgat
     6001 atctgctggg tggaagaagc ggaagcggta acgaaggaat cgtgggatat cctgattcca
     6061 accatccgta agccgttctc tgaaatatgg gtaagtttca acccgaaaaa catcctcgac
     6121 gatacctatc agcgattcgt cgtaaatcct cccgatgata tttgcctgct gacggtgaac
     6181 tacaccgaca acccgcattt tcctgaagtc ctccgtctgg agatggaaga gtgtaaacgc
     6241 agaaatccga cactgtatcg tcacatctgg cttggtgagc cggtaagcgc aagtgatatg
     6301 gcaatcatca aacgtgaatg gcttgaagcc gcaaccgatg cgcacacgaa actcgggtgg
     6361 aaagcgaaag gtgcggttgt ttctgcgcat gacccatcag atacagggcc agatgctaaa
     6421 ggttatgcat cgcgtcacgg ttcggtagtt aagcgcattg ccgaaggtct gctgatggac
     6481 atcaacgagg gtgctgactg ggctacttcg ctggcgattg aagacggcgc tgaccattac
     6541 ctgtgggacg gtgatggcgt cggtgcgggc ctacgcagac agacaacgga agcattctcc
     6601 ggcaagaaaa ttaccgccac gatgttcaag ggtagcgaat cgccattcga tgaagatggg
     6661 ccttatcagg ccggagcatg ggctgatgaa gtcgtacagg gcgacaacgt tcgcactatt
     6721 ggcgatgtat tccgcaataa gcgagcgcaa ttctattacg cgctggctga caggctgtat
     6781 ctgacatatc gggcggttgt ccacggtgag tatgcagacc ccgacgacat gctgagcttc
     6841 gacaaagaag cgataggcga gaagatgctg gagaagctgt ttgcagaact gacgcagatt
     6901 cagcgcaaat tcaataataa cgggaagctg gagcttatga ctaaggtcga aatgaagcag
     6961 aagctcggta tcccatctcc taacctggct gatgcgctga tgatgtgtat gcattgcccg
     7021 gcattggtcc gcgaagaaac agaaatatac gttccctcat cctccggttg gtaaacatgg
     7081 cagagacatt agagaaaaaa catgagcgga tcatgctcag gtttgatcgc gcctattctc
     7141 cacagcagga agtgcgcgaa aagtgcattg aagctacgag gtttgctcgt gtccccggag
     7201 gtcaatggga aggagcaacg gcggctggaa ctaagcttga tgagcagttc gagaagtatc
     7261 ctaagtttga aatcaataag gtagcaactg aacttaaccg catcattgca gaataccgca
     7321 ataacagaat cactgttaag tttcgtcctg gtgacagaga ggcaagcgaa gagttagcca
     7381 ataaattaaa tggtctgttc cgtgctgact acgaagaaac cgatggcggt gaggcttgcg
     7441 ataatgcatt tgacgacgct gctactggtg gtttcggttg cttccgtttg acgtcgatgc
     7501 tggtcaatga atacgacccc atggacgatc gtcagcgtat tgctattgaa ccaatatacg
     7561 acccgtcgcg ctctgtgtgg tttgaccctg acgctaagaa gtacgacaaa tctgacgcgt
     7621 tgtgggcgtt ctgcatgtat tcgttgtcac ctgaaaaata tgaggctgaa tacagaaaga
     7681 aacctcctgc ttctctggat gtaacgtcta tgaccagttg ggaatatgac tggtttgatg
     7741 aagatgttat ttacatagcg aagtattacg aagttcgtaa agagtctgtt gtcgtcatca
     7801 gttatcgaca tccaatcact ggagagattg caacatacga cagtgatcag gttgaagata
     7861 ttgaagatga actggcaata gctggatttc aggaagtggc aaggcgctca gtgaagcgcc
     7921 gtcgtgtgta tgtatccgta gtggatggtg atggtttcct tgagaaacct cgacgtattc
     7981 ctggtgagca tatccccctc atcccggttt atggaaaacg ctggttcatt gatgacattg
     8041 agcgtgtcga aggacatatt gcaaaagcaa tggatccaca gcgtttgtat aaccttcagg
     8101 tatcaatgct ggctgatact gcagcgcaag accccggtca gatccctata gttggcatgg
     8161 agcaaattcg tggacttgag aagcactggg aggctcgcaa caagaaacgc ccagcgttct
     8221 tgccgttgcg cgaagtgaga gataaatctg gcaacattat cgctggagct accccggcag
     8281 gatatacaca gcctgcggtt atgaatcagg cattggctgc attactacag caaaccagtg
     8341 ctgatattca ggaggttaca ggcggcagtc aggccatgca gcagatgcca agtaatattg
     8401 ctcaggaaac ggttaacaac ttgatgaaca gagcagatat ggcttcgttt atctatctgg
     8461 acaatatggc gaaaagtctt aaacgcgctg gtgaagtatg gctgtcaatg gcgcgtgaag
     8521 tgtacggttc agagcgtgaa gtgcgcatcg ttaacgaaga tggaagtgat gatatcgctg
     8581 tcctgagcgc acaggttgtt gacaggcaaa caggggctgt tgttgcgtta aatgaccttt
     8641 ctgtcggtcg atacgatgtg acggttgatg ttggaccaag ctacacagca cgacgtgatg
     8701 caacggtttc tgtactgaca aatgtcctta gctctatgct tccaacagac ccaatgcgcc
     8761 cggcaattca gggtattatt ctggacaata tcgatggcga aggccttgat gacttcaaag
     8821 agtacaaccg aaaccaactg ctgatatctg gtattgcaaa accacgcaat gagaaagagc
     8881 agcagattgt tcaacaggcg caaatggcag cacaaagcca gccaaatcct gaaatggttc
     8941 tcgctcaggc gcaaatggta gcagcgcagg cagaagcgca aaaagcaact aacgaaactg
     9001 ctcaaactca aatcaaagca tttactgccc agcaggatgc gatggagagt caggcaaaca
     9061 ctgtctataa actggctcaa gccagaaaca tcgatgacaa agcagtgatg gaggcaatac
     9121 gccttctgaa agatgtcgcc gagtcacaac aacagcaatt ccagtcacca ccacagtcac
     9181 cggcagactt aatgccgagt taaccaggag taatcaatgg aaaacgaact gatcatcgac
     9241 ggtcaggtta ttgacctgtc tgaaacacag gaaaatgcag aagaaaccat catccaaaca
     9301 gagtcacagc ctgagaatga aagccaggat gacaacggaa aagagatggc aactgatcct
     9361 gaaaaaaccg aagagacacc agaagattac gccttgcgta ttggtgatga agaaattcag
     9421 cttaacgctg acgataatga tcacattgac gggcaacctg caccgcaatg ggtgaaagat
     9481 cttcgcaaag gcttcaaaga aacacagaaa gaaaaccgtg agttgcgacg ccagcttgag
     9541 gaagcattag ccaagcatgc ggaacatcag caaccacaac cagacgctat tccaccaaaa
     9601 ccgactcttg agtcgtgtga ttatgacgaa caggcgtttg aacaggcatt gactgattgg
     9661 catgagaaaa aaggccgtgt cgaacagcag cagcaacaaa aactacgtca gcaacaggaa
     9721 taccagcagc gtttccagca aagggtagaa gcgcataaac aacgggcagc caaacttcct
     9781 gtgaaagatt atcaggaaat ggaagccatt gttcttagtg agctaccacc aattcagcag
     9841 gaaatcatca ttcactgtgc agacgaaggc tctgaactac tcgcctatgg cttaggtaag
     9901 agccagcaat tacgccagcg tgtagccgct gagacagatc caattcgcgc agcattcctc
     9961 ttggggcaga ttagcaaaca ggtaagcctt gctccaaaac caaagaaagc catcaagcca
    10021 gagccggaag tacgtggtgg cggtgctgat gcgaaacaag acgaattcaa caaattatgc
    10081 cccggcgcaa aaatcgaata aggaaaagat aaatgcctaa caatctcgac agtaacgtca
    10141 gtcaaatcgt tctgaaaaaa ttccttccgg gttttatgtc agatttagtt ctggcgaaaa
    10201 ccgtagaccg tcagttgctg gcaggtgaaa tcaactccag cactggcgat agcgttagct
    10261 ttaaacgtcc gcatcaattc tcatccctcc gtactcccac tggtgatatt tcagggcaaa
    10321 ataaaaacaa cctgatctca ggtaaagcta cggggcgtgt aggtaactac atcactgttg
    10381 ctgttgaata tcagcaactg gaggaagcga tcaagcttaa ccagctggaa gaaattctcg
    10441 cgccggttcg ccagcgaatc gttaccgacc ttgaaacaga gcttgctcac ttcatgatga
    10501 ataacggtgc gttgtcactt ggtagcccca atactccaat caccaaatgg tctgatgttg
    10561 cgcagacggc atctttcctg aaagacctcg gcgttaatga aggtgaaaac tatgctgtaa
    10621 tggatccatg gtctgcacag cgacttgctg atgcgcagac tggtttgcat gcttcagatc
    10681 aattggttcg tactgcatgg gagaacgcac agatcccaac caattttggc ggcattcgcg
    10741 cactgatgtc taatgggctt gcctctcgta cgcagggggc atttggcgga acactgacag
    10801 tcaaaacaca gccaactgtt acctataacg cagttaaaga ctcataccag ttcactgtaa
    10861 cattgaccgg agcgacagcc agcgttacag gttttctgaa agctggtgat caggtcaaat
    10921 tcaccaatac ctactggctg caacagcaga ccaaacaggc gttgtataac ggagccacac
    10981 caattagctt cactgcaacg gttactgctg atgctaattc agacagcagt ggcgatgtga
    11041 cggttacgct ttctggtgtt ccgatttatg acactacaaa cccgcagtac aactctgtaa
    11101 gtcgtcaggt agaggcaggc gatgccgtat ctgtagtagg cactgctagc cagacaatga
    11161 agccaaacct gttctataac aagttcttct gtggacttgg atctatccca ctgccgaaac
    11221 tgcacagtat tgattctgct gttgcaacat atgaaggttt ctccatccgc gtacataaat
    11281 acgcagatgg cgatgccaac gtgcaaaaaa tgcgcttcga cttactgcct gcatatgtgt
    11341 gctttaaccc acacatgggc ggtcagttct tcggtaatcc gtaataacaa ggggcttacg
    11401 ccccttttat gttttaagga aacaatatgg atcggatgag tgtattcctt gccgcagata
    11461 acgaatccgg gcatgtacag gccgttatcg cagaaaaaga cttccagttt ttcgaaaggt
    11521 tgggctttgt tgcctcagtt gatgaattga aaccgaccag taagcgaggt cgtaaggcgg
    11581 cggacaatgg caacagtact gacaaagggt gagatcgtcc tttttgcgct tcgtaagttt
    11641 gctattgctt ctaatgcatc gcttactgat gttgagccgc aatcaattga agatggtgta
    11701 aatgatctgg aagatatgat gtccgagtgg atgattaacc ccggcgacat tggttacgct
    11761 ttcgcaactg gagatgagca gccattacca gatgatgagt caggtcttcc aagaaaatac
    11821 aaacacgcag taggctatca gttattgctg agaatgctat ctgattacag ccttgaacca
    11881 actccgcaag ttctcagtaa cgcccaacgc tcatatgatg ccttgatgac cgacactctg
    11941 gttgttcctt caatgcgacg acgtggagat tttcctgtag ggcagggtaa taaatatgac
    12001 gtgttcacat ctgaccgata ttatccaggc gatctccctc tgattgatgg cgatatccca
    12061 aacgcatagg tgaataaatg cctattcagc aacttccgct tatgaaaggt gtcggcaaag
    12121 actttagaaa cgccgactat atcgactatc tgccagtgaa tatgttggct acacccaaag
    12181 aaatcctgaa cagcagcgga tatcttcgct cattcccggg cattgccaaa cgttctgatg
    12241 tgaacggcgt atcgcgaggc gtcgagtaca acatggcaca gaatgctgtt tatcgcgtgt
    12301 gtggtggcaa actgtataag ggcgaaagtg aggtcggtga tgttgccgga agtggtcgcg
    12361 tatcaatggc gcatggtcga acatcacagg cggtaggcgt taacgggcaa ctggtcgaat
    12421 accgctatga tggcacggtt aaaaccgtct caaactggcc tgcagacagc gggtttatgc
    12481 agtatgagtt aggttctgtt cgcgacatta cgcgtttacg tgggcgttat gcgtggtcaa
    12541 aagacggcac tgattcatgg tttatcactg accctgaaga cgaatcgcat cctgaccgct
    12601 acagcgcaca atatcgagcc gagtctcagc ctgacggcat catcggcatc ggaacatggc
    12661 gagacttcat cgtctgcttt ggttcatcga cgattgaata tttctccctg acgggggcaa
    12721 ccaccgttgg tgccgctttg tatgtcgcac agccatcgct gatggtgcag aaaggcattg
    12781 ccgggactta ctgcaaaacg ccgttcgccg attcgtatgc gttcatcagc aatccggcaa
    12841 caggtgcgcc gtctgtgtat atcatcggct ccggtcaggt atcaccaatc gccagcgcga
    12901 gcattgagaa aattcttcgc tcctacactg ctgatgaact ggctgatggt gtgatggaat
    12961 cgctgcgatt tgatgcgcat gaactgctga ttatccacct tccgcgccac gtcctcgtgt
    13021 acgacgcatc ttcaagcgcc aatggtccgc aatggtgtgt gttgaaaaca ggcctgtatg
    13081 acgatgtgta ccgcgctatc gacttcattt acgaaggaaa tcagataacg tgcggcgata
    13141 agctggaatc ggttaccggg aaattgcagt tcgatatcag cagccagtat gggctacagc
    13201 aagaacacct gctgtttact ccgttgttca aagcggataa cgccagatgc tttgatctgg
    13261 aggtggaatc atcgacgggt gttgctcagt acgctgaccg cctgttcctc tctgcaacca
    13321 ctgacggcat aaattacggg cgtgagcaga tgattgagca gaatgaaccg ttcgtttacg
    13381 acaaacgcgt tttgtggaag cgagtagggc gcatcaggaa aaatgttggc ttcaaactgc
    13441 gcgttatcac taagtcgcct gtcactctgt ctggcgctca gataaggatt gagtaatggc
    13501 ggattcgaat ctcaatgtgc cggtaatcat tcaggctaca cggctcgaca catcagtcct
    13561 tccacgcaat atcttctcgc agtcgtatct gctttacgtt atcgcacagg gtactgatgt
    13621 tggtaatgtg gctaacaagg ccaacgaggc cggacagggc gcttatgacg cacaagtcag
    13681 gaacgatgag caggatgtga ttctcgctga ccatgagcag cgaatttctg ctgcggaagc
    13741 aacgcttgtt aatcatgagg agcgaatcag ccaggcagaa tcaactcttc aggaacatga
    13801 aacgcgaatc gctcagaatg aaagcgatat tgcgtcgctt gataccagag ttcagtcgct
    13861 ggaatcgcag gtttcagacc atgaatcgcg cattgatgct ctggagtatg ccactactcg
    13921 caagaagtca gaggttgttt actctggcgt atctgtaacc atcccgacag cgccgaccaa
    13981 ccttgttagc ctgctgaaaa cgctcacgcc gtcatcaggc tcgttggcac cattcttcga
    14041 caccgttaac aacaagatgg ttgtgttcaa cgagaacaaa accttgttct tcaagctgtc
    14101 gatcgtcggg acgtggccca gcggaaccgc caacaggtca atgcaactaa cattttccgg
    14161 ctctgttcct gacacactgg taagcagtcg caactcggcg acaacgaccg ataacatcct
    14221 gttagctacg ttcttcagcg tggataaaga cggctttctt gccacaaatg gcagcacgtt
    14281 aaccattcag tcaaatggtg cggcgtttac tgccacaacc atcaagataa tcgcggagca
    14341 gtaatgattc agttcaaacc aacgcgaaac atcgacctga tagaagcagt cggaaatcac
    14401 cctgacatta ttgccggaag caacaacggt gatggatacg actacaagcc tgaatgccgt
    14461 tactttgagg ttaacgtgta cggtcagttt ggcggcattg tttactatca ggaaattcag
    14521 ccgcttacat tcgattgcca cgccatgtac ctgccagagg ttcgtggatt cagcaaggaa
    14581 atcgggctgg cgttctggcg atacattctg actaacacca ccgttcagtg cgtcacatcg
    14641 ttcgctgcgc gcaaattccg ccacggtcag atgtactgcg caatgattgg ccttaagcgt
    14701 gtaggaacca tcaagaaata cttcaaaggc gtggatgacg tgacgtttta cagcgccaca
    14761 cgcgaagaac taatcgactt cctgaatcac gggagatagc tatgttatat gcatttaagc
    14821 tgggcagaaa actgcgcggc gaggaacctt attgccctga aaaaggcggg aaaggtggca
    14881 gctctgataa aagcgcaaag tatgcagcag aagctcagaa gtatgccgca gacctgcaaa
    14941 atcagcagtg gcagacgatc atgaaaaacc ttgctccgtt cacgccgctt gcggagcagt
    15001 atgttaacca gcttcagaat ctttccagtt tagaaggtca ggggcaggca cttaatcagt
    15061 attacaactc tcagcagtat aaagaccttg caggtcaggc tcgttaccag agtcttgctg
    15121 ctgcggaggc gacgggtgga cttggttcga cagccacaag caatcaactg gctacgatcg
    15181 cgccgacact cggtcagtct tggttatcaa atcagatgag caattacaac aatctggcaa
    15241 acgttgggct tggtgcactg caaggtcagg caaacgccgg gcagacgtac gccaacaaca
    15301 tgagcagcat tgcacagcaa agcgcagcac ttgccgctgc taatgccaat aaaccatcaa
    15361 gtcttcagac tgcaattagc ggcggcacat ctggtgcgat tgccggtgca ggtcttgcca
    15421 gccttttggg aacatcaacg ccttggggcg ctggcattgg tgctggtatc ggattgcttg
    15481 gctcgttgtt ttaaggggta atcatggcta cttggcaagg aacaaacggc ggattgttgg
    15541 ctggtatcgg cggcgtcaac tcaaacgctc cgagcgtaaa tgacatcggc aatacgcttc
    15601 agcttatcag gcagaacaat gatattgatc gttcaggcgc taacaatgtt gggctgactg
    15661 ctttgcaagg cctttcaggt attgcagggg tgtttcagca ggaaaagcag gctcagcggc
    15721 agaaagaatt tcagcaggcg tacgctaatg cttatgcgtc tggtgatcgc ggtgctttgc
    15781 gtcagttggc tactcaatat ccagaccaga ttgaatccgt tcgtaaaggc atgggattca
    15841 ttgatgaaga ccagcgtaat tctatcggca ccttagcggc tggcgcacgc cttgcgtcat
    15901 cgtctccaga agcaatgcaa tcatggctgc aaaacaacgc caaggaactg actcgcgtcg
    15961 gtgttgaccc taacagcgtt gctcagatgt atcagcagaa cccttcagga tttggtgagt
    16021 ttgttgatca ccttggaatg gctgctcttg gtccaattga ttacttcaat gttcaggaca
    16081 agatggctgg tcgtgaaatt gaccgaggca gactggcaga gacaatccgc agcaatcagg
    16141 ctggagaagc acttcaggcg agagggcaaa acctttccta tcagtcagca atgactgggc
    16201 acaatatcgc agcacaacgc ttggctctgg atcagcaaga gttcgggttt aagatgcagc
    16261 aagcgcagga aaaggctcag cagttgatta gcgaagcacc taagctgtca gtaaacatgg
    16321 aaaaaggcat cgagacggct gtaaacaatg ctacagcatc atcaaactca gccaattcta
    16381 tgagtgcgct tgctcaacag ttcagagcag aaaaaccaac gacaggtttg ttcggtaacg
    16441 cacagaacat gttcgcaaaa cttaccggaa gcgatacaac attgcgtgat ttgcgcattc
    16501 gccaaaatgc ccttgttaac agtcaggttc ttaaattcct acctcccggc ccagcaacgg
    16561 ataaagacgt tgagatcgtt cgacagggtg cgccaactga catggataac cctgagacgg
    16621 tcgcaagatg gcttgatgca atggcaaacc ttgagcgacg aaacgcgcag tttaatgagt
    16681 ttaaagccga gtggatgagc gcgaatggca accctggaca atcgcgtaat ggcggtcaga
    16741 tattggggtt ggatgttaaa aaaggtgaat cattggggag tgccgttaag cggtatatgt
    16801 caatgaatac tgacgcagcg ccagcacaag attcgacacc ttcaggagaa ccacggaatc
    16861 aggttggatc atatacctca aaatcaggca ttcaatttac ggtggaatga tgaaagtaac
    16921 tgcaaacggt aagacattta cctttcctga tggtacgagc accgaagata ttggcaccgc
    16981 cattgatgag tattttgctg gtcaggctgt tcagcaacaa acagttaatc aggccaataa
    17041 tgcaccaaca cgggacgaac catcattgat gcaacaagct ggcgattggc tcactggtgg
    17101 tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt ctggtaaaca taccatttga
    17161 cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt caggggcttg gtgggccaaa
    17221 agttttggat gatgtttatc gtccagtaga caaaccgaca gacccatacg cacaagccgg
    17281 tgaaacaatt ggtgggtatt tagttccagg agttggaacg gcaggaagca tggctattgg
    17341 atcgctggca gaggccgcaa atcagaaagg cgatttcgca caaaatgcag ctaaaaatgc
    17401 cggagttaac cttgccgctc agggtgttct ttccgcagca gcaaagggaa tagggcgtgg
    17461 aataacggct ataaaaggtg atattgcgcc agaagtggcg aagaaaattg ccacatcaga
    17521 atcgatgggc gtgacaccaa tgacatctga tgttatcccg ccgaaaaatg ctttcactcg
    17581 cggccttact caggatgccg agggggcttt gctcgggacg ggctcaaagc gagcagagca
    17641 atatgcaacg cgtagtaagc tggtaagcaa ttattttgac cgttttggtg agtacaaccc
    17701 tgatgatgtg gtgaaatctc tgaccaccac gttaaggggg cggaaggatg ctgctggcgc
    17761 tgttatcaat gacgtcacca ataaaatggg taatgccgca gttgatacta caaataccat
    17821 gaatgctctg aatacagcga tcgcaagaca ggaacggctt gggacttcag ccaatcaaag
    17881 cctgcttaca tccttgcgta acctgcgtga agaattagca aaccctgcaa ctgatttgga
    17941 tgttacgttt gatctcttgc gtcagcacag aacagcattt agatctaatg ttcagggaga
    18001 tgctatggtc ttccccaacc aggcaaaagc agctaccaat atggtagaga atgcaatgtc
    18061 aaaagacctt cgtaacgcag ttgcaaaaaa cctcggtgcg tcagacgcag caaaatacct
    18121 taaagcaaat tccgattatg caaacgttta taataaggtg cttaataaaa acattgctaa
    18181 caagctcaac aaggcaagca gtgaagccag tcctgaactt ataaatactg ttgtattaag
    18241 cagaaaacca tctgacgtga aacgaatctg gagcgcattg gatgataaag ggaaagatgc
    18301 tatgcgtgca gcttacgtca gcaaaatagc ggaaaaggcc ggtgactctc cagccaagtt
    18361 catcactgaa gttaataagc tgaaatctca gtcaggtggt gaaatttaca acactatttt
    18421 ttctggaaag cacatgaaag agcttgattc tcttcatgaa gttctacaga aaacagcaag
    18481 gtcagacacc gcaaatgtag taactcagac tgggcaatcg caagccaaca ggataaggac
    18541 gattggcgca actgcgactc ttggcgtatc aatggggctt gaggctggtt tcggtgcaat
    18601 gatgcgcttg tatgagtcca aagcagcaag gaatgctctc ttacgtttgg caaacaccaa
    18661 agcaggaaca ccagcctatg aaagagcgct aaataatgct gcaaatgcga tacgccctat
    18721 actctcaagc caaattacag cagaacagca ataaaagata agatgtaact acctgatatt
    18781 actgctactg ttgcatgtta ccgtgtttcc aaatcctgaa ttgcagtttg tatatgtgtc
    18841 aacgcgtgtt gggtaaggtt gagttataac aggctggcgc gctttttgct cgatcgcttg
    18901 cattgtgttt acagcctgat aattcaataa agcctgctgg aatgcttggc tttgtgctat
    18961 ttgttgggct tgttcttggc tttgtaattg aacataaaga ttctgaagct caagtcttgc
    19021 ctgtgcgtca cttatcttgc cttcatcgac acctttcccg agcatctttg cagcaaggac
    19081 atacagctta ggtgttggtg ctgatgccat gcgtgagtcg ttcttcacac tggcatcaag
    19141 gcaattagcc atatcgctaa gcttttgata gcgttgttcg caacttgctt gatagtcact
    19201 tacttttgcg catccaacca gcagaaacgg gataattaac agtgattttt tcatatggtt
    19261 aactctcctt agtttttcac aggatatcat gaaggcaatg ccattttagc cggaaactag
    19321 atttctatgt ttccttttta ttattgctat acatggtctt aagcgtttca aaaaccattt
    19381 tcttaaccat atcagattgt tgttctgcca tacgctctgc atcatcaatg taaacggatg
    19441 cagagctttg tttagccaac gattcttcaa tcgctgcaat tatctctgag ttcagcgacc
    19501 tgttattcat cttcgcgcgc tgtttaattt tcgcgtggag ttcatgcgga agtctcaagt
    19561 gaaactgcgc ctcgtcgtat ttgctgtaca tccttgatgc ctcaccagtt gggtggaatg
    19621 gcatcgtaac ctactggata aatactcaat agtaccattt cggtatgcaa tcacatcatg
    19681 gttgcatcat atcattcttc tggagcaatg aaatgtcaga tatcaccgca aatgttgtgg
    19741 tcagcatgcc ttcgcaactc ttcactatgt ctcgctcttt taaagccgta gccaatggaa
    19801 aaatttatat cggtaaaatt gacactgacc cggtaaatcc agaaaaccag attcaggttt
    19861 atgtggaaaa cgaagacggt tctcacgttc ctgtttcgca accaatcatc attaacgctg
    19921 ctggttaccc tgtatataac ggacagattg ccaagttcgt aactgtgcaa ggccattcta
    19981 tggctgttta tgatgcatac ggtgcgcagc agttctattt tccgaatgtg ctaaagtatg
    20041 atccagatca gtttaagggc aatctcctga gcgaagatca ctcacttggc gactctctag
    20101 tcatgcataa cccacagttt gtcgatagca catcgaccat tctctcaaaa aagctaacta
    20161 tcgacctgag gacgcttaca gactacgggt ttgctgaggg aaacaccggt gctcagaaca
    20221 aagctgcatt tcagaaggct attgatgatg ctacgttgcc aactgagatt gttataccag
    20281 agggggcgtt cattgttgac cctggtatta cgattaagaa tactgtcacc atgatacgag
    20341 gtgctggcgc atatcagtcc agaattttct ctacgggtac agcggcaccg ataatcacgc
    20401 aacaagatgg ggtaattacg ttctgcgagt tcagggactt tggtcttgac ggaaatggct
    20461 acgcagctaa cggcatcagt cttactgaag ccaaccacat taaaatagaa aatattgacg
    20521 tggttaatac taataacaac gcaatattag ttaacggtta ctccattgac atcattgggt
    20581 gccggttgtt tcaaaacgcc ggtaacggta ttaatgttgg tggttactgc aataatatta
    20641 atataatcaa cagccgtatc tatggtaacg gagccggtgg agttttactt acgccagctt
    20701 acgctgaggg cggtatgagc gtcagagtta atggcaacaa tatagagcaa aataagttct
    20761 acggactttt ggcttacggg gttaaaggcc ttaaccttga tgctaactat tgggaacgca
    20821 acggtgagat tggttacccg tatagtgtac ctgagtctat tacagtacgt gcggatatcc
    20881 accttatcgc gaacaatttt acattaattc ctgacctttc aaaaattaat gataccgtat
    20941 ctattcgcgg gaatcagcag accgcgattg gctacgccag cgcgctacct aaccaagacg
    21001 gatttatttt cacgaactat gcgaaaaacc tgacgattga aaataatcag ttattagacg
    21061 catcgaaagt gaataattta ttagcaatgt accacaataa tctttcgtcc aaagttactg
    21121 acaggctata tcttgccaac aacacagtga actccatcgg atacgttggt agttatgacc
    21181 cggcgacaca aaaccctgac actgcgcatt taatagatat tgcgaacaga gagttaactg
    21241 ctaactatct tgatcgaaat atgttgctct ggactgcggc atctggaact acaggaacat
    21301 tgataaaaac tcaaaacatc tatgctggta attattcatt cttagttacg acaggagata
    21361 gagtttgggg acgaacgata gacctcaaca aatcaccaga actaaaaggg aaatttgtat
    21421 ggtttggtgc gtgggtcaat gaccagggat cagcgtcaaa acttatgttt ataattaatg
    21481 gtgcaggaca aacagactct acagcacctt tagctggtaa tgggaaatgg agctatgtta
    21541 gttgtggtgt gtatatatac gaaactgata cagctataaa tgttggtata agaaattatg
    21601 gttcaggaaa tgtactcata aactcacctt ctctatgtgc atatggaatg ccatcaaacg
    21661 cgttgcaagt tgaaaaaaca acattttatc tttcgtcagt acctacatcc ggtttctggg
    21721 atataggtga gcgtgttatc aatagcgcac ctgcttcagg acaaccaaag gcatggacat
    21781 gcaatattcc aggtgggcca ggaacctatt ctttcttatc agagggaaac ttttaaaaga
    21841 cctatgtatt gaaatgatta ctttgatctg gtatgtttaa aaaaaatcag taaaacacat
    21901 tttgatatgg aaagaaaaac aaataagtta gactctattc aagtccttag agggatcgcg
    21961 gtacttctag tcattgcgtt tcactttaga gtatatttaa atggagtgta tgcccaaaag
    22021 gacttgggcg acattctttt tggtataggt gaggttggtg ttgatatatt ctttgttata
    22081 agtggattta tcattactta ttcttcaatg aataaggcaa aaaaccggcc aatggtgttt
    22141 gctgctaagc gtttttttag gttgtaccct gtttacttta taatccttac tctggctata
    22201 tacctcaact atgaggcaat atatacatca tcacaaataa taaagagcta cttgctcata
    22261 ccaattgact ataattttat agggccatgg tcaggttata gtataataat acctgcatgg
    22321 acactaacgt atgaaatttg gttctatgtg gttttctttt tttcattatg catcagccat
    22381 aaatatagaa ctgcgatcgc atcatttctt ctcatcatta tttgcgttct tggtcaactg
    22441 tactttcacg gagatgtttt tctaaatcct atcgcgcaac cgccatctgg tgatggaatt
    22501 attcgtaatc tggtatttgt atcaaatcca ataatatatg actttatttt ggggatgatt
    22561 attgcagagg cctacattag gctaccttct agacttataa ataaaatgcc tgttgttcaa
    22621 tttcttaata ttacattagc gattgccttg ctttttttat gtctagggtt aaggaatgga
    22681 cctggaattg tagca
//
LOCUS       DAZFJF010000076        22208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFJF010000076 DAZFJF010000000
VERSION     DAZFJF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.076"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(202..2469)
                     /gene="adiA"
                     /locus_tag="ACHKAU_003139"
     CDS             complement(202..2469)
                     /gene="adiA"
                     /locus_tag="ACHKAU_003139"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO5532150.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2668..3576)
                     /gene="melR"
                     /locus_tag="ACHKAU_003140"
     CDS             complement(2668..3576)
                     /gene="melR"
                     /locus_tag="ACHKAU_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO5532151.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3859..5214
                     /gene="melA"
                     /locus_tag="ACHKAU_003141"
     CDS             3859..5214
                     /gene="melA"
                     /locus_tag="ACHKAU_003141"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO5532152.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5317..6738
                     /gene="melB"
                     /locus_tag="ACHKAU_003142"
     CDS             5317..6738
                     /gene="melB"
                     /locus_tag="ACHKAU_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO5532153.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6877..7506)
                     /gene="yjdF"
                     /locus_tag="ACHKAU_003143"
     CDS             complement(6877..7506)
                     /gene="yjdF"
                     /locus_tag="ACHKAU_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO5532154.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7629..9275)
                     /gene="fumB"
                     /locus_tag="ACHKAU_003144"
     CDS             complement(7629..9275)
                     /gene="fumB"
                     /locus_tag="ACHKAU_003144"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5532155.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9353..10693)
                     /gene="dcuB"
                     /locus_tag="ACHKAU_003145"
     CDS             complement(9353..10693)
                     /gene="dcuB"
                     /locus_tag="ACHKAU_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO5532156.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11264..11983)
                     /gene="dcuR"
                     /locus_tag="ACHKAU_003146"
     CDS             complement(11264..11983)
                     /gene="dcuR"
                     /locus_tag="ACHKAU_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO5532157.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11980..13611)
                     /gene="dcuS"
                     /locus_tag="ACHKAU_003147"
     CDS             complement(11980..13611)
                     /gene="dcuS"
                     /locus_tag="ACHKAU_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO5532158.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13792..14022
                     /gene="yjdI"
                     /locus_tag="ACHKAU_003148"
     CDS             13792..14022
                     /gene="yjdI"
                     /locus_tag="ACHKAU_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO5532159.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            14034..14306
                     /gene="yjdJ"
                     /locus_tag="ACHKAU_003149"
     CDS             14034..14306
                     /gene="yjdJ"
                     /locus_tag="ACHKAU_003149"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5532160.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14533..14829
                     /gene="ghoS"
                     /locus_tag="ACHKAU_003150"
     CDS             14533..14829
                     /gene="ghoS"
                     /locus_tag="ACHKAU_003150"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5532161.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14857..15030
                     /gene="ghoT"
                     /locus_tag="ACHKAU_003151"
     CDS             14857..15030
                     /gene="ghoT"
                     /locus_tag="ACHKAU_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO5532162.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15149..16666)
                     /gene="lysS"
                     /locus_tag="ACHKAU_003152"
     CDS             complement(15149..16666)
                     /gene="lysS"
                     /locus_tag="ACHKAU_003152"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5532163.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16903..18360)
                     /gene="dtpC"
                     /locus_tag="ACHKAU_003153"
     CDS             complement(16903..18360)
                     /gene="dtpC"
                     /locus_tag="ACHKAU_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO5532164.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18419..20566)
                     /gene="cadA"
                     /locus_tag="ACHKAU_003154"
     CDS             complement(18419..20566)
                     /gene="cadA"
                     /locus_tag="ACHKAU_003154"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO5532165.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20646..21980)
                     /gene="cadB"
                     /locus_tag="ACHKAU_003155"
     CDS             complement(20646..21980)
                     /gene="cadB"
                     /locus_tag="ACHKAU_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO5532166.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         5635 a         5311 c         5552 g         5710 t
ORIGIN      
        1 acaaattttt atagtaattt ctcatgtaca tattttttta caggataaat aacaggcaaa
       61 aaaaagcgcg gggtggggcc gcgcaaaaag tatgacattt ttgtttgcag caatttttat
      121 gaaaacgttg ccatactcaa aaggcctaag gccaggcggc gtaatgttat ttaaacaatt
      181 acgccttcag cggaatagtg gttacgcttt cacgcacata acgtggtaaa taccgtcaat
      241 aatttcagtc ccttcagttt cgtgttcaaa tccagggaaa tggtggtccc aggattgcag
      301 cgagcgtaaa taacttactt gcggactgtt tttatcgccg aagttttcac cagacagcag
      361 catcgggatt cctggcggat acgggataac tgagtttgcc gcgatgcgtc ctggcagatt
      421 ttcaatggat accagttcga cattgttgtc gacaatcgcg ttgtacgctt cacgcggggt
      481 gatttccgcc atcggcaggc cggaataggc ttcgttcaac cgtgcgccag ggttgttttc
      541 tttcagccag gcaaacatgg tgtcacccag atcgtgaatc cccatgttcg cgtaagtgtc
      601 aggatattgt tcaacaagtt ccggcatcac ctgcgccagc ggtgtgttgg cgtcatagtg
      661 gcgtttgaag gaacaaaggg tgttaaccag agtcccccat ttcccacggg ttacgcccat
      721 agagaacagg aacataattt ggaagtcagt ggtgcgggta ggcacaatgc cgtggcgacc
      781 aagccaggca gtgaccagcg ccgccggaac accggtttct tccagttcac catcttcacc
      841 cattcccgga gcaaggatgc tgactttaat tgggtcgagc atactccagt tatccggaat
      901 atctttgaag ccgtgccagc tttcgcccgg atgcattacc cagcagtcct gaacggtggt
      961 cagcagtttg gttggtgcgt cagcaaagtc ataggttttg ccggtttgtg ggtcggtgac
     1021 gacttctttg ttccacggtt tgaagaacca gctaccgtca gcggtgaact ctttatatag
     1081 ccgcgccatc gcctgacgga aatcaaccgc ttcgtcaatc acttcctgtg tcagtgacag
     1141 gccgctgttg ccgtccatca tcgacaccgc cacgtcgttg gatgcgcaga tggcatacag
     1201 cggggaggtg gtggcatgca tcatgtaggc ctggttgaag cgggagaagt taatcgcccc
     1261 acgaccttca cgtacatgaa tataagaagc ctgtgacagc gcattcagca gtttgtgggt
     1321 ggagtgggtg gcgaaaacgg taggaccgtt gtgatcgcca ggttcgccgc gcatggcata
     1381 gtgatcggca tagatcgggt tgaaacgtgc atagccgtac caggcttcgt caaagtgcag
     1441 acgatcggag gttttttcca gcagatcctg cgcttcttta gcgttataac acacgccgtc
     1501 ataggtgcag ttggtcacca cgcagtaaga cggtttttgc ccggctttgt ctttggtcag
     1561 cgggctttca ctgattttct tctgcaaggt ttcaggttgc atttcctgcg gatagattgg
     1621 cccgataatg ccgtagcggt tgcggcttgg caccatatag accggtttcg cgcctgtcag
     1681 catcaagcct tgttcgatgg atttatggca attacggtca acgaccacga catcgttatc
     1741 ggtcatgcaa gcctgcatga tggtgcggtt agagccggaa gtaccgacga ctaccgacca
     1801 ggagcgatcg gcaccaaata cgcgtgcggc atatttttcg ctttcgccaa atgcgccagt
     1861 atggtcaagc aaagaaccga gggaagttcg ttcgatgccc atgtcggtgc ggaacagatt
     1921 ttcaccatag tagtcatggt agaaacgtcc ggcgggtgtt ttggtaaaac caacgccgcc
     1981 ctggtggcct ggcgctgccc aggaatattc atggatgtca ctatatttca tcagcgcgct
     2041 gaacagtggc ggcaacagct gctggcggta gcgggtcatc gcggcaacgg cgcgtccggc
     2101 gataaagtcg gcggtatctt ccagaatcca ggcgaattca tcgacaagct ccagcagatc
     2161 ccggtccatt gcggcgaggg ctttttcccg atcgcccaac aggaagaccg gcacgttttg
     2221 ttggcgctca tgaagcttac cgatcaattg tctgacgttt tgatgttcgt ccggatgttc
     2281 catttgatag ctgaacatca ggcagtcaat ggcttcgttt gaagagagaa tggcaaaacc
     2341 atcatcaaag gaggtggatt taatcacggt aacattttgc tggcttaaag catctgccag
     2401 acgctcaacg gcgttaccga cccaggtgtc ttgatggaga aactcgcttt caacaattaa
     2461 tactttcatc attgcttacc cggttatgaa ggaatcttcg ttgcgggcaa gtatcttccg
     2521 gccttttcgg gctgtaaagc gcgtgaaaaa caaacatttt tgaaatattt agttttataa
     2581 acaatacgtt atgtgattat tttaaattat tttcactgct tattatcgac ggctaaacta
     2641 tttttttggc tgatactgat atcgtcttta gccgggaaac gtctggcggc gctgttggct
     2701 aagtttgcgg tattgttgcg gcgacatgcc gacatatttg ccgaacgtgc tgtaaaaacg
     2761 actacttgaa cgaaagcctg ccgtcagggc aatatcgaga atacttttat cggtatcgct
     2821 cagtaacgcg cgaacgtggt tgatgcgcat cgcggtaatg tactgtttca tcgtcaattg
     2881 catgacccgc tggaatatcc ccattgcata gttggcgtta agtttgacgt gctcagccac
     2941 atcgttgatg gtcagcgcct gatcatagtt ttcggcaata aagcccagca tctggctaac
     3001 ataaaattgc gcatggcgcg agacgctgtt tttgtgtgtg cgcgaggttt tattgaccag
     3061 aatcggttcc cagccagaga ggctaaatcg cttgagcatc aggccaattt catcaatggc
     3121 gagctggcga atttgctcgt tcggactgtt taattcctgc tgccagcggc gcacttcaaa
     3181 cgggctaagt tgctgtgtgg ccagtgattt gatcaccatg ccgtgagtga cgtggttaat
     3241 caggtcttta tccagcggcc aggagagaaa cagatgcatc ggcagattaa aaatcgccat
     3301 gctcttacag gttccggtat ctgttagttg gtgcggtgta caggcccaga acagcgtgat
     3361 atgaccctga ttgatattca ctttttcatt gttgatcagg tattccacat cgccatcgaa
     3421 aggcacattc acttcgacct gaccatgcca gtggctggtg ggcatgatat gcggtgcgcg
     3481 aaactcaatc tccatccgct ggtattccga atacagcgac agcgggctgc gggtctgttt
     3541 ttcgtcgctg ctgcacataa acgtatctgt attcatggat ggctctcttt cctggaatat
     3601 cagaattatg gcaggagtga gggaggatga ctgcgagtgg gagcacggtt ttcaccctct
     3661 tcccagagag gcgaggggag cgtccgagca tcatgaggcc gaaaactctg cttttcaggt
     3721 aacttattac cataaactca gatttactgc tgcttcacgc aggatctgag tttatgggaa
     3781 tgctcaacct ggaagccgga ggttttctgc agattcgcct gccatgatga agttattcaa
     3841 gcaagccagg agatctgcat gatgactgca cccaaaatta catttatcgg cgctggttcg
     3901 acgattttcg ttaaaaatat tcttggtgat gtgttccatc gcgaggcgct gaaaacggcg
     3961 catattgccc tgatggacat tgatcccacc cgcctggaag agtcgcatat tgtggtgcgt
     4021 aagcttatgg attcagcagg ggccagcggc aaaatcacct gccacaccca acagaaagaa
     4081 gccttacatg atgccgattt tgtcgtggtg gcatttcaga ttggcggtta tgaaccttgc
     4141 acggtgactg atttcgaggt ctgtaagcgg catggtctgg aacaaaccat tgccgatacg
     4201 ttggggccgg gcggtattat gcgcgcgcta cgtaccattc cgcatctgtg gcaaatttgc
     4261 gaggacatga cggaagtctg ccccgatgcc accatgctca actatgttaa cccaatggcg
     4321 atgaatacct gggcgatgta tgcccgctat ccgcatatca aacaggtcgg gctgtgccat
     4381 tcggtgcagg gaacggcgga agagctggcg cgtgacctca atatcgaccc agctacgctg
     4441 cgttaccgtt gcgcaggtat caaccatatg gcgttttacc tggagctgga gcgcaaaacc
     4501 gccgacggca gttatgtgaa tctctacccg gaactgctgg cggcttatga agcagggcag
     4561 gcaccgaagc cgaatattca tggcaatact cgctgccaga atattgtgcg ctacgaaatg
     4621 ttcaaaaagc tgggctactt cgtcacggaa tcgtcagaac attttgctga gtacacaccg
     4681 tggtttatta agccaggtcg tgaggatttg attgagcgtt ataaagtacc gctggatgag
     4741 tacccgaaac gctgcgtcga gcagctggcg aactggcata aagagctgga ggagtataaa
     4801 aacgcctccc ggattgatat taaaccgtca cgggaatatg ccagcacaat catgaacgct
     4861 atctggactg gcgagccgag tgtgatttac ggcaacgtcc gtaacgatgg tttgattgat
     4921 aacctgccac aaggatgttg cgtggaagta gcctgtctgg ttgatgctaa tggcattcag
     4981 ccgaccaaag tcggtacgct accttcgcat ctggccgccc tgatgcaaac caacatcaac
     5041 gtacagacgc tgctgaccga agctattctt acggaaaatc gcgaccgtgt ttaccacgcc
     5101 gcgatgatgg acccgcatac tgctgccgtg ctgggcattg atgaaatata tgctcttgtt
     5161 gacgacctga ttgccgccca cggcgactgg ctgccaggct ggttgcaccg ttaaaacgcg
     5221 actaaacgct actgcgccgg gggatttatt ccggcgcaca cctctgacga taccaataac
     5281 agaaggcggg cgttggtaac agcgacccga taccctatga gcatttcaat gactacaaaa
     5341 ctcagttatg gatttggagc gttcgggaag gattttgcga tcggcattgt gtatatgtac
     5401 ctcatgtatt actacaccga tgtcgttggg ctgtctgtgg gtttggtcgg tactttgttt
     5461 ctggtggcga ggatctggga tgctattaac gatccgatta tgggatggat tgtaaatgct
     5521 acgcgatcgc gatggggtaa gttcaaaccc tggatcctga tcggtacgtt ggcaaactct
     5581 gtaatcttat ttctcctctt tagtgcgcat ctgtttgaag gtactactca gattgtcttt
     5641 gtttgcgtga cctacatcct ctggggcatg acttacacca ttatggatat tcccttctgg
     5701 tcgctggttc caaccatcac gctcgataaa cgtgagcgcg aacaactggt tccttatccg
     5761 cgtttttttg ccagtctggc aggctttgtt acggcaggtg tgacgctacc atttgttaat
     5821 tatgtcggcg gtggcgatcg gggatttggc tttcagatgt tcactctggt actaatcgcc
     5881 ttttttattg tttcaaccat catcactctg cgcaatgtgc atgaagtctt ttcgtcagac
     5941 aatcaaccgt ctgctgaagg aagccatctg acacttaaag ccatcgttgc gctaatttat
     6001 aaaaacgatc agctttcatg cctcttgggt atggctcttg cttataatgt agccagcaac
     6061 attattaccg gatttgctat ctattatttc tcatatgtta tcggtgatgc ggatttgttc
     6121 ccctattatc tgtcgtatgc gggagctgct aacctggtga cgttagtatt cttcccacgc
     6181 ttagttaaat cattatcccg acgcatttta tgggccggag catctattct tccggtgtta
     6241 agctgtggtg ttctcctgtt aatggcatta atgagctatc acaacgtcgt cctcattgtg
     6301 attgcgggta ttttgctgaa tgtgggaacg gcgcttttct gggtattaca ggtcatcatg
     6361 gtggcagata ccgttgatta cggtgaatat aaactgcacg tacgctgtga aagtatcgct
     6421 tactccgtgc agactatggt ggtgaagggc ggttcagcct ttgcggcttt tttcattgcg
     6481 gttgtgttag ggatgattgg ctatgtaccg aatgttgaac agtctacgca agccctatta
     6541 ggtatgcagt ttattatgat tgctctacca actctgtttt tcatggtaac gctgattctc
     6601 tacttccgtt tctatcgcct caatggcgac acgctgcgca ggatccagat tcatctgctg
     6661 gataaatatc gcaaagtacc gcccgagcct gttcatgctg atattccggt cggtgcagtg
     6721 agtgatgtga aagcctgacg tggacaatag ctaacgaaaa tggcctgatg cgatatgttt
     6781 atcaggccac gtcttaatgt aatgctttgg atttgcaaat tttgtagacc ggataaggaa
     6841 ttcacgccgc atccggcatc aacaaagcgc acgttgttat ccggttatca agccaaagcg
     6901 ccgtagctgg cggcaatgaa agcgagcgag gaatatcacc gtcgttaatg cgccaagcag
     6961 cgcgcagaac atatcggatt gcgtgtccca ctggtcgccc tgggtgccga gaaaatcatc
     7021 cgctccctgg cccattgcca gcgccgccca ccactcgatt aattcataca tcgcgcttat
     7081 cgccagcgcc acgcagcaga ccaggaacgc caccatttta cgtccgcgca cgtacatccc
     7141 gcgcacgaga atttctcgtg ccaccagtgc aggcaccagc ccctggaaaa aatgccccag
     7201 cttgtcatac ggattacggc tcaaccccag ccattcctgc acctcaaaac cgatggggac
     7261 tttcgcgtag gtgtattgcc cgccgaccat caggatgatg gcgtgaaaga aaatgagcgt
     7321 atagagcagc ggcgttaacg gataacgttt agcggtggca agcagtagcg gcacgacaat
     7381 aatcaccggt gtcacttcca tcagccaggt gagtttgtca tgggccgaaa tgccggtata
     7441 aatcaggatt aacgttagcg ccagtgcgcc ggtgttaaga attaacggct tgagtgtacg
     7501 ggtcatggtg agttcgcaag tcagggaaaa tactgactat tcaccagcaa gcgtgaaatt
     7561 acaatcgcaa aagaggtggc ccagggggat cacctggcag catgctgcca ggcgctgggc
     7621 cgaagaggtt acttagtgca gttcgcgcac tgtttgttga cgatttgctg gaagaagtcg
     7681 ttacctttgt catcgaccag gataaacgcc gggaaatctt ctacttcgat tttccagata
     7741 gcttccatac ccagctccgg ataagcgacg cactccagat gcttgatgct ctgctgcgcc
     7801 agtaccgccg ccggaccgcc gatgctaccg aggtagaagc cgccgtgttt atgacacgcg
     7861 tcggtaacct gctgactgcg gttacctttc gccagcatga tcatgctgcc gccgtgggat
     7921 tgcagcagat ccacgtagga gtccatacgg cctgcggtgg ttgggccaag tgaacctgat
     7981 ggataaccgg caggggtttt cgccggaccc gcgtagtaga tcgggtgatc tttgatgtac
     8041 tgcggaagtt ctttaccggc gtcaatcagc tctttcagct tggcgtgtgc aatatctcgg
     8101 cccacgataa tggtgccggt gagcgacaaa cgagtggata ccgggtattg cgaaagctgg
     8161 gcgaggatct ctttcatcgg gcggttaagg tcaactttca ccgcttcgcc ttcaccggcc
     8221 tggcgcagtt cttgtggaat gtactggcct gggttgtgtt ccagtttttc gatccagata
     8281 ccttcgcggt tgattttcgc tttaatgtta cggtcagcgg agcaggagac gcccatgccg
     8341 accgggcagg atgcgccgtg acgtggcaga cggataacgc gaatgtcgtg cgcgaagtat
     8401 ttaccgccaa actgcgcgcc aagaccgagt ttctgggcct cttccagcag ttcctgttcc
     8461 agctggacat cgcggaacgc ctgaccatgt tcgttccctt ccgtcggcag ttcatcgtaa
     8521 tagtgagcgc ttgctaactt gacggttttc aggttggttt ccgcagacgt accgccaatc
     8581 acaaacgcga tatggtacgg cgggcaggct gcagtaccga gggtacgcat tttctcgacg
     8641 aggaagtttt tcagtttgcc gggagtcagc agggctttgg tttcctggta gagatacgtt
     8701 ttgttggcag agccgccgcc tttcgcaacg caaaggaatt tgtactcatc gccatctacc
     8761 gcgtacaggt cgatttgcgc aggcaggtta gtgccggtgt tgacctcttt gtacatgtcc
     8821 agcgccgcat tctgtgaata gcgcaggtta tcttcgatat aggtgttata gacgcctttc
     8881 gacagcgctt cttcatcacc gccaccggtc cacacgcgct ggcctttttt accgacgatg
     8941 atcgcggtgc cggtatcctg gcaggtcggc agcacgcctt tggcggcgat ttcggagttt
     9001 cttaagaatt gcagcgccac gtacttgtcg ttttcgctgg cttctggatc gtgaagaata
     9061 gccgcaacct gtttctggtg tgccggacgg agcatgaaag aagcgtcgtg aaaggcttgc
     9121 tgcgccagta gggtcagggc ttctggttcc actttcagga tggtttcgcc gtcgaagtcg
     9181 gcaacgctaa cgtaatcgga agtgagtaga tagtattcgg tattgtcttt ccccatcggg
     9241 aaaggtgcct ggtagataaa gggtttgttt gacatagctt ccagcctgta actctgtatt
     9301 tgttattcga aaatggcgtg ccgcgtagtc acgggcggca cgcaaagtgc atttataaga
     9361 acccgtacat cgcggcgaag atccagccga agacgcacga tacgctcaca ccaatcaacc
     9421 ccggcagaat aaaactgtgg ttgatgacga agcgaccgat gtgggtggtg ccggaacggt
     9481 caaactgaat cgctgccaga tcgctcggat aagtcggcag gatgtaataa ccgtagcaag
     9541 ccggtgctga agccacgatg tatgccggat caacgccgat cgccagcgcg accggaacaa
     9601 tcgccgccag cgccgcagcc tgagagttta caaacttgga aaccagcagc agaacaatgg
     9661 catacgccca cggatactct ttcaccattt cacccagtac gccctgaatt tcagacatat
     9721 gcgcaccgaa catggtttct gccatccatg cgataccgta caccgccacg atggcgatca
     9781 taccggaacg gaagacttcg ttttttgaga tagacgcggg attggttttg gtcaggataa
     9841 taatcagcgc cccggtcagc agcataaaca tctgaataac cagtaccatc gacagcggtt
     9901 tgccgccgaa ggatggacgc aggtccgaat cagcaccaag aagggcgact acagcgattg
     9961 caccgaggaa aatccacatt gccagccagt tgcttttcgg cagtttttta tcgagcagcg
    10021 tcgcggtatc accgtaaaca tactcacggt tttccggtac ggagatgaat ttctggaact
    10081 cttcgtcttt atccagatct ttaccgcgga accagctgaa gataccgatc gccaggatac
    10141 cgattaacgt cgatggaatg gtgattgcca gcaggtcgag gaactcaaga tggcgaccat
    10201 caaaggtgac attacccagc attgcaacca gagacacgac cgcaaccgac accggactgg
    10261 cgataatccc catctgtgca ccgatagaac ttgccgccat cggacgttcc ggacggatgt
    10321 tgttcttaat ggcgacgtcg tagatgatcg gcagaatggt gtaaaccaca tgacccgtac
    10381 cgcaaagaat ggtcagtgta caggtcacaa acggcgcgac aattgagaca tatttcgggt
    10441 tgcggcgcag cagcttctcg gcaatttgca gcatgacatc aagaccgccc gaagcttgca
    10501 aggtcgccga tgccgccacc accgcaatga taaccagcat gacatcaacc ggtggtttac
    10561 ctggctgaag gtggaagacg aagaccagaa tgaccagacc gataccgcct aataaaccca
    10621 gcgcgatacc accctttctg gcaccataaa acagacatat cagtattatg ataagttgga
    10681 tagtaaataa catgtgtgaa ccctcgcgat aatcctattt aaatttttgc tgaatagatc
    10741 acagtcacgt tctgttttgt atgaactgtt ttcagagcct gactaaatat ccgtttggtc
    10801 gttaccggct ttagcaaata cctcacagta aatattggct gattaaagca gcggcaaatt
    10861 tatgcactgt gataagcggc tttttcagag agaaaaactc tctggcgatg ggtttatagt
    10921 ttgtactgcg cagatgttaa tgttttgttt gctgttatct tattgatatt tatcggtcta
    10981 ttttttgttc tcagggcaga tatcattagg tttaaagatt aatctgatct acccatttgt
    11041 gggtaaaaat acacataatg cgggtgacat aatagttaat taacttttgt tatcgttttg
    11101 aaattaaaaa caccgttcac ctgaagagat attaattttt agcgatgatg gagggataat
    11161 tatatttgat ctggcacaag ttttactgat gaaggatgta acttgtgcca ggggtatttt
    11221 gcattacggt aataattatt acttacagat aaccagcgat ccgttattgg caatattgtt
    11281 tcagtagtga gtagtgttct gcctgaatac ggtaacggta aaccggacgc cccgtgacgc
    11341 cataatggat actggtgaac aagatgtggc agttgaccag ccagatgagg tatttacggc
    11401 aggaaacacg cgagatgtta acctcgttag ccagctcgtc ggttgaaaac tcatagtcct
    11461 gatgcgcgtc aatccactgg cacagcgtgc gtaaagtctg cggcgttaag ccttttggca
    11521 agcgacgagg atcctgttcg ttggagctgc tgccgtggat tagctgatca agctcggcct
    11581 ggtcataata ctgatgtttt tccagcgcca ttttcttttg ccgccagccg gtgagcgcct
    11641 cttcaaagcg ggaagcctgg aagggtttga tcaggtaatc cacgacaccg taatgcagcg
    11701 aatctttaat ggttgccgca tcggctgcgg aggagatgac aatcacatca cttttgcaac
    11761 gcgcgttatg caggacaggc agtaaatcga gcccgttctc tttttgcata tagatatcga
    11821 gcaatatcag gtcgataggc gtatcgctat tgaagataat ctctttggct ttctccagcg
    11881 tcgaggctgt tccacagcat tgaaagcctg ggatttgtgc tacgtatcgg cgattcagct
    11941 ccgcgaccat tgcgtcgtca tcgataatta atacattgat catctgttcg acctctcccc
    12001 gtcccagggt atctggacaa aaaattgtgt gaaaatcccg ggttccgatt ccacggcgat
    12061 gctgccgccg agattttcta cctgttgttt gacaagtgct aaaccgacgc ctcgctcgct
    12121 tccttttgtc gagacacctt tgtcaaaaat gtgatcgatt ttgtcgggtg cgattcccgg
    12181 tccatcatcg ttaacttcgc agtgcagcca gccgtgacgg tagtgcaatg ttacgctaat
    12241 ttcgccaccg ggttccggcc ctaatgcttc cagcgcgttt tctatcagat ttcccaatgt
    12301 ggtaatcagc gtcgcgacct ggtcctcact gccgctgtct ggcagctggc tttcactgtt
    12361 taaaatcagc gtatggccta aatcggtcgc gcggttaatc ttgctgatta aaaaaccagc
    12421 gataaccgga gatttgatct tacccagcag agagccaatc tcttcctgat agttattggc
    12481 tgttttgaga atgtaatctt ccaactgctt ataactcttc agatgcaata atccgagaat
    12541 cacatgcaat ttattcataa attcgtggga tcgttcacga agtgcatcag catagttgac
    12601 cagaccgtcg agtcgctgca tcagtttacg tacttcagtt ttgtccctga aggttgaaat
    12661 ggcaccgatg ataacgccat tactgcgcac cggaacggtg ttgatcagta atagccggtc
    12721 tttaatcgta atctcttcgt cgcggcgcgg cgtaccgtcg cgtaacactt ccgagacatc
    12781 taccacctgt gaccatgagt ggctgagcgt cgacagtttc tcatcgtcct gcgacttacg
    12841 gtaattcagc aattcttgtg cggcatcgtt gatcagcgtg acctcgccgc gatcgtccac
    12901 ggcaacgacg ccttctttga tagactgcaa catggcctgg cgctgctcaa acagcgtgga
    12961 gatttcgtag ggttccaggc cgaaaaggat ttttttcagt accttaacca gaatgcaggt
    13021 gccaatcagt ccgaccagca tgccaaataa taccgaccag ataatgctcc agcgactgtc
    13081 attgatttgt tgggtcacgc ggcttaactc aaggccgatc gccaccacgc caatttgttt
    13141 atgattttca tcgtagatgg gggtaaatac gcgtaaagcc tgcgccagaa aaccgcgatt
    13201 gatagcgaca ttttctttgc cattcagcgc tttaaggatg tcatcacctt taaatggctg
    13261 accaatacgc tgggcttcag gatgcgagta gcgaagactt tgcatatcgg taacgacaat
    13321 aaacagcaga tcgttgcgtt tgcgcacggc ttccgcgatg gcctggattc cgctctcctg
    13381 cggttttttc tgcaagccct gacggatttc cggcgagtcg gcgagggtac gcgccactgc
    13441 cagtgccttg ttggccagcc cgtctcgcgt catatcactg atttgcgaga agtaaatcag
    13501 atgcaccacc aatagcaccg agaacagtac cgcgctgacc attaagatca ctgtggtact
    13561 caatttcatc ggacgtttgc gtaacatgcg gtagggcaat gaatgtctca tcagcttcct
    13621 tgtgtgacaa atttcttaag cattatctct gatgaggcgg gtaattcaaa gggagtaaga
    13681 atgattggct atatagggga agagactctg gcaacggaaa ctgccagtgc tgtatgaaga
    13741 ttccagggct atgcttatag cgataatcat actgatgaga gagggaaagt catggatcag
    13801 gcgctactgg acgagggtta tcgctgttat accggcgaaa agatcgatgt ctatttcaac
    13861 actgcgatat gtcagcattc tggcaattgc gtacgtggca acggcaagtt atttaatctc
    13921 aaacgaaagc cgtggatcat gccggatgaa gtcgacgtcg tcactgtggt taaagtgatt
    13981 gatacgtgcc cgagcggcgc gctgaaatac cgtcataaat aagcgagggt aaaatggaaa
    14041 tacgcgaagg ccacaataaa ttttacatta atgacgaaca aggcaagcaa atcgctgaaa
    14101 ttgtctttgt gccgaccgga gagaatttag cgattatcga acataccgat gtcgatgaaa
    14161 gcctgaaagg gcaggggatt ggtaaacagc tggttgcgaa agtcgtggaa aaaatgcgtc
    14221 gggaaaaacg aaaaattatc ccactatgcc catttgcgaa acacgaattt gataaaacgc
    14281 gggagtatga tgatattcgc agttgatggg agagtacaga gtcacgatat ttttcatgct
    14341 ctccgcggag tgatgcagga gagcatctga agggtagggg gatgcacaaa gaatgggcag
    14401 agagcgcgtt tttttgtccc aagtcatccc ctttactgag caaaaaaaag aatatctcct
    14461 atatgagaat catcaatcgg ggttaataat ttttgcgtcc ccagagcatt caatattgat
    14521 aggagtcata ttatggaagg taaaaacaag ttcaatactt atgttgtttc ttttgattat
    14581 ccatcatctt attcctcagt gttcttaagg ttaagatcat tgatgtatga tatgaatttc
    14641 tcttctatcg tggctgatga atatgggata ccacgacaat tgaatgaaaa ttccttcgca
    14701 ataacgacat cgttagccgc aagtgaaatc gaagatttaa tcaggcttaa atgcttagac
    14761 ttaccggata ttgattttga cctcaacatt atgacagttg atgactattt ccgtcagttt
    14821 tacaagtagc caggaaggat aagaagaaca taaaaaatgg cactattctc taaaatatta
    14881 attttttatg tgattggtgt gaacatatcc tttgtcatta tctggtttat ctcacatgag
    14941 aaaacacata ttcgtttact tagtgcattc ctggtcggaa taacctggcc aatgagtctg
    15001 cctgtggcat tacttttttc tctcttttag gagctgtagt tgagtggacc cgctggttat
    15061 cgtgaaacga agcgtctctc ctcaaaactg gatttgcgga acgacgaacg aagcagtcgc
    15121 ctgactgctt cgttcattaa agtgaaattt atttctgtgg gcgcatcgcc gggaagagaa
    15181 taacgtcgcg aatagtatga ctgtttgtaa acagcataat cattcggtcg ataccaatac
    15241 ccagaccagc ggttggcggc agaccatatt ccagcgcagt cacgtaatct tcgtcataga
    15301 acatggcttc gtcgtcacct gcagctttag cgttaacctg ttcctggaaa cgttcagcct
    15361 gatcttctgc gtcgtttaat tcgctaaaac cattaccgat ttcacgacca ccgatgaaga
    15421 attcaaaacg gtcggtgatt tctgggttaa catcattacg gcgtgccagc ggtgacactt
    15481 ctgccggata ttccgtaata aaggttggct gaatcagatg tgcttctgcc acttcatcaa
    15541 agatctccgt gacaatacgt cccagccccc agcttttctc taccgtaata ccgatagatt
    15601 cagctaatgc tttagcagca tcaaaattat ccaggtcggc catatcggtt tctggacgat
    15661 attttttgat tgcttcgcgc atggtgagtt tttcaaacgg tttgccgaaa tcaaacacat
    15721 gctcgccata agtgacttta gtggtaccca gaacctcttg tgccagggtg cggaacaggg
    15781 actctgtcag ttcaatcaaa tcgtggtaat ccgcatacgc catgtagagt tccatcattg
    15841 tgaactcagg attatggcga acagaaatac cttcattacg gaagttacgg ttgatttcga
    15901 atacccgttc aaaaccgcct acaaccagac gtttcagata cagctccggc gcgatacgca
    15961 ggtacatatc taaatccaga gcgttatgat gggtaataaa cgggcgagca gatgccccac
    16021 ctggaattac ctgcatcatc ggggtttcta cttccataaa gccgcgcgcg accatgaact
    16081 ggcggatagc ggccagaatt tttgaacgga caacaaacgt ttgacgggat ttatcgttag
    16141 cgatgaggtc cagataacgt tgacgataac ggacttcctg atcctgcaga ccatggaatt
    16201 tatctggtaa aggacgtagt gcttttgtca gcaggcgcaa ctcagtacag tgaatggaaa
    16261 gctcaccggt ttgcgtctta aacagcgtac cgcgggcacc gataatgtca cccagatccc
    16321 attttttaaa ctgatcgtta taaacacctt ctggcaggct atctcttgca acgtacagtt
    16381 gaatacggcc accgacatcc tgcaacgtta caaaggaggc tttccccatg atacgacggg
    16441 tcatcattcg gccagcaacc gagacttcaa tgtttaagga ttccagttcc tggttatcct
    16501 tcgcatcaaa ctcttcgtgc agctggtcag aggtatggtc gcggcgaaaa tcattgggaa
    16561 acgccacacc ttgctgacgt agtgccgcca gtttttcgcg gcgatttctc agttcatcgt
    16621 taaaatcaat agcctcattg gctccccgtg tttcttgttc agacattttg gttcctctaa
    16681 atccagcttt caatttagcg tagataaaga gacagatcgg tacactgtat tatctgcctc
    16741 gactattaat aactcagttt atcttatcga aactaacgct tacctcaagt tgatatcact
    16801 aataaataac catcaaaatg gtttaaatga cttattaatc tagatttgct agtgttataa
    16861 actgaaagtt aatttattca taaccaattg tttttacgac agttaatcgt tgctctcctg
    16921 tatcatattc gtcggcgtgc taaacagaaa acgggtagca aaggccaata cgacgatgat
    16981 cgcgacacaa gccaacgtcc actctcccat ctgagaaaag aatcgctggt aagctgcaat
    17041 tgccataccg ctaatttgcg actctgtcgt ctgctgtgcc acgacgcctg ccagccagtt
    17101 ggcgaccgcg cctgttgcca gcatataaat accggttaat acgccagaca ttttcagacg
    17161 cgtgatttgc gcaatcgcca ccgggtcaat aaagagttcg gcaaagccca ttagcgccag
    17221 cccggatatc atcacggcca ttgacgcttg accgccagcc gctgcatgtc gggcatcaaa
    17281 tgccaacaac ataaagccac aagccatcag cagtaagcca aaggcaaact tcagccagac
    17341 gcgcaatgtt gagttgccgc ggctttctgg cgacgccagc caggccagta caaccccagc
    17401 gagcatcact gcaattgcat tcaccgactg gaatagtgct gtaggtactt caatattgaa
    17461 tgcctgacga ttcacaaagc gatcgataaa caagctgatg gtactgccgc cctgttgtgc
    17521 cagtacccag aacaacgtcc cgacaaacat caacaataca atttgccaaa gagcacggcg
    17581 gtgttcgggg aatttgatca tcatgcgggc aatgatttgt gcggcaatga ggcaaacgat
    17641 cgccagcaaa tatccggacc agtcgttctc cagcagcagg gtaaaaaata ctggggctaa
    17701 acagagcatc accactaacc agctccatac tggtaaggca aatttcacgc tagtgagcgc
    17761 ttttttatcc atactacgcg tggactggaa atgacgatgc ccgcttaaga aaatcaacaa
    17821 accgataaac atgccgccac ccgcaagggc aaagccaaca tgccatccat accactgagc
    17881 agccaggccg caggcgattg gggcggcgat agaaccgata ttgcccgcag catacagcag
    17941 cgaaaaaccg ccatcacgtc gatgatcgtt ctcgtcgtag agctcgccaa gcaaacagct
    18001 gatgtttgat ttgaataaac cgtagccaca aataatgatt gccagcgcca gatacaggct
    18061 aaaggttgaa tttgtatcaa tgcccagcac cacatggcca agggtcatta acagcgcgcc
    18121 ggcaatcact gcagtgcggt tgccgagcag gcggtcggca agccagccgc cgagaatagg
    18181 ggtaacgtaa accagagaag catatgcgct gaacaggctg atggcatggt tatcatcaaa
    18241 accaagctga tgggtgagat agagaatgag taaggcacgc atgccgtaaa aactgaagta
    18301 ctcccagatt tggatcgcca cgatatagta tatcgcgcgc ggctgtgagg gtgttttcat
    18361 gtgttctcct tatgagcaaa aaagggaagt ggcaagccac ttcccttgta cgagctaatt
    18421 attttttgct ttcttctttc aataccttaa cggtatagcg gccatcagcc tgacggtatg
    18481 caccgtgaat atcggtttca aagcccggat agtgagcgcc gatttcacac agcatctgca
    18541 ggaactccag aaccggacgg ctttcttcgg tgatcatttc acccggcatt accagaggaa
    18601 ctcccggcgg atacggaagg atcatattgg cgttaatacg acctaccatt tcgtcgaggt
    18661 aaacttcttc ggtcataccg tgcagctctt tctggaatgc agcatacgga gtcattacca
    18721 tcgtcggcag cacttcaaat gcgcggtaca tcagatccgg cagattgtgg tgaacaatca
    18781 gtttgtggat attctgagcc agttcctgaa tacgcatgtt ttcatagaat tcaggatctt
    18841 cacgatacag agacggcagc atgtttttca cacgcaggtt caggtcgaac gcacgtttga
    18901 agtcagtcag agcacgcagc aggctcagtg ctttggtctt atcgataccg atgctgaaca
    18961 ggaacagcag gttatacgga ccggttttct caacaacgat gccatgttcg tcgaggtatt
    19021 tcgccacgat gctggccgga ataccaaagt cgctcatggt gccgtctttt tccatccccg
    19081 gagtcagcag ggtgactttg atcgggtcaa gatacatgtg ctcgttatcg atgtttttga
    19141 agccgtgcca ggtgctgtca gaacgcagcg gccagcattc agtcgtatcg atatgatccg
    19201 gctgccatac atcaaagaac cagccatcag attccgttct cagacgtttg atctctttac
    19261 ggaatttgat cgcacgttca atggaaccgt tgatcagacg tttacctgca ttgcctttca
    19321 tcatcgccgc agcggtttca gtggacgcca cgataccgta gtgcggagaa gtggtggtgt
    19381 gcatcatgta ggcttcgtta aaggtttctt cgtttacgtc acctttaacg tggatcatgg
    19441 aagcctgaga gaacgccgcc agcagtttgt gagtggactg ggtttcgtaa atcactttcc
    19501 cttctacacg gccaccgctc ataccgcatt taccttcgta aatcggtgag aagttggtgt
    19561 aaggcaccca cgcggagtca aagtggatgg atttcacatc cagtgttttc ttgatgaagt
    19621 cggtgttgta cagcagacca tcataggtag agttggtaat tacagcatgt accggccagg
    19681 ttgcgtttgg tgtttctttc acgcgcttag caatggtagc gtgctggaat tcactctgtg
    19741 ggataccacc aagaataccg taagcgttac gggtcgggcg gaaatagatt ggcgtaacat
    19801 cgctcatcat catcaggtgg gtcagcgatt tgtggcagtt acggtcaatc agaatggtgc
    19861 tgcctgccgg agcagagtac atgccaacaa ttttgttcgc agtggaagta ccgttggtca
    19921 ccatgtagct gcggtctgcg ttaaagacgc gagcgatata ctgttctgct tctttgtgtg
    19981 gaccactgtg atccagcaga gaacccagtt cagatactga aatggaaata tcagatttca
    20041 tggtattcgg accaaagaaa tcatagaaca ggctacctac cgggcttttc tggaatgcag
    20101 taccgcccat gtgaccagga gtacagaaag tatatttacc ttcacgaaca tatttaaaca
    20161 gtgctttagt cagcggaggc agaatagtgt tgatatattc gtcagtggtc tgcttgatct
    20221 tattagcaat atcttcagca gcacccagcg catattcaaa gaagctaatc tgtaaacgca
    20281 ggtcattcag gcttacatcg agagtggaat acgtattagc gaacgcgtac aacggcaggt
    20341 tctcgttcat tttgctaatt tcttcgcaca gctcgagatt atatttatcc cagtcaaaaa
    20401 taacgccgca cagacgcgca ttgttttcga tcagttttaa taagtcgtca cggtcgttcg
    20461 ggtaaacaat ctggaagttc agacgttcaa gcgcgcgatg aagttcacgg atgggttctt
    20521 ctttaaaata aacccccatg tgattcaata ttgcaataac gttcatagtc atatctccag
    20581 gtaaaaaagg cccctcccaa cacatgggac aaaatgaaag gaggagcctc ggaaaatact
    20641 tttaattaat gtgcgttaga cgcggtgtgg ttatccattg agtggctctg gcgctcgtgc
    20701 attttgcgag cgtagaacat caggataatc aggctgacga tgaaggtacc tgccagctcg
    20761 aaggagcttg cgcccatcag cgcgatgaag cagaacacac aacccagtac agagcagatc
    20821 aggctgacaa agttgcggat gttaacgcct tcaaaacgaa tcaggtcaac gcaagagtag
    20881 aagtacggca gcatagtcag cagtactgcg ataccggtca gttcaccgaa caggtcagat
    20941 gctttaccac cggcagagtt catcagagtg atgaggatca tcagggcagt cattttcact
    21001 gcagccagca gcagaccttt tttcggaata ccgttgctgt cgacttcacc ataaactttc
    21061 gggaagttac cgtcgttagc ggcacgtaca cctgcctggc ctaccaacat catccaggag
    21121 cccagagaag tcaggcacgc aaaggcggtg aatgcagaaa ccagcggcgc agcccagtta
    21181 ccgaggatgg ttgaagcact gattgcaaac ggagcaccgg aagctgccat tacagaagat
    21241 ggatacatac cggaaagcac ctgagtcgca gcgatgtaaa caatacctgc taaaccagta
    21301 cccagcatgg ttgccagcgg aacggtacgt ttcgggtttt taaccatacc agtacttact
    21361 gctgcggatt caacacccac gaaggcccac aggcagagca gaatactttt aatgatcgca
    21421 tgaccatcag tggtatccgc agtattccag ttagctgcat aagttgccgc atcaaaccaa
    21481 tgccagccaa caatagcggt cattactacc ggaataagaa ccagcaccag accaatagtg
    21541 gttaaacggc ttacccaagt accgccgagc atatttacaa aggtaaatac ccagacgata
    21601 gcaatacagg cgatacccgc cggaacagga tcatttaata ctgggaagaa ggtggaaaga
    21661 taagataccg cggtaatacc aatcgccagg ttaccaatcc agttagcatg gtaataaaga
    21721 acacctgtct gaaaaccaaa tgcaggggaa atttctccgg cataagcaat tgggccacct
    21781 tgttgcgggt tttttgttgc cagtcgggca tatacatacg ccagcgacat tgcaccaata
    21841 atagagataa tccaacccca gatagcaata ccaccgatac ttgctaggtt cgcaggtaat
    21901 aatgcaatac cgctccccat catattaccg gcaacaacac cggtacaggc aaatagcccg
    21961 atcttcttgg cagaactcat gctcttctcc taatttcatt tttgaatttg gagtccgggt
    22021 catgatgtat aactatttcc tgaccagacc aaactggcgc taagaatact cacgaaaaaa
    22081 ggattaatcc taaagattag gtgaaataac acaaaagttt ctgtaagtga gaacttgagg
    22141 ttttttatta acacatcagg atcgcaagtt gatatcatga aaagataaac attcaatgtt
    22201 tacaatgg
//
LOCUS       DAZFJF010000077        20984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFJF010000077 DAZFJF010000000
VERSION     DAZFJF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.077"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            234..884
                     /gene="csgD"
                     /locus_tag="ACHKAU_003156"
     CDS             234..884
                     /gene="csgD"
                     /locus_tag="ACHKAU_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO5532167.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            889..1278
                     /gene="csgE"
                     /locus_tag="ACHKAU_003157"
     CDS             889..1278
                     /gene="csgE"
                     /locus_tag="ACHKAU_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO5532168.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1303..1719
                     /gene="csgF"
                     /locus_tag="ACHKAU_003158"
     CDS             1303..1719
                     /gene="csgF"
                     /locus_tag="ACHKAU_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO5532169.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1746..2579
                     /gene="csgG"
                     /locus_tag="ACHKAU_003159"
     CDS             1746..2579
                     /gene="csgG"
                     /locus_tag="ACHKAU_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO5532170.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2643..3134)
                     /gene="ycdZ"
                     /locus_tag="ACHKAU_003160"
     CDS             complement(2643..3134)
                     /gene="ycdZ"
                     /locus_tag="ACHKAU_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5532171.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3236..3790)
                     /gene="ycdY"
                     /locus_tag="ACHKAU_003161"
     CDS             complement(3236..3790)
                     /gene="ycdY"
                     /locus_tag="ACHKAU_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO5532172.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3814..4551)
                     /gene="ycdX"
                     /locus_tag="ACHKAU_003162"
     CDS             complement(3814..4551)
                     /gene="ycdX"
                     /locus_tag="ACHKAU_003162"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO5532173.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4606..5544)
                     /gene="ghrA"
                     /locus_tag="ACHKAU_003163"
     CDS             complement(4606..5544)
                     /gene="ghrA"
                     /locus_tag="ACHKAU_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO5532174.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5778..5865
                     /locus_tag="ACHKAU_003164"
     tRNA            5778..5865
                     /locus_tag="ACHKAU_003164"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5812..5814,aa:Ser,seq:gga)
     gene            complement(6015..6857)
                     /locus_tag="ACHKAU_003165"
     CDS             complement(6015..6857)
                     /locus_tag="ACHKAU_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5532175.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6942..7139)
                     /locus_tag="ACHKAU_003166"
     CDS             complement(6942..7139)
                     /locus_tag="ACHKAU_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5532176.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7151..7642)
                     /locus_tag="ACHKAU_003167"
     CDS             complement(7151..7642)
                     /locus_tag="ACHKAU_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5532177.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7639..8013)
                     /locus_tag="ACHKAU_003168"
     CDS             complement(7639..8013)
                     /locus_tag="ACHKAU_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5532178.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8060..8434)
                     /locus_tag="ACHKAU_003169"
     CDS             complement(8060..8434)
                     /locus_tag="ACHKAU_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5532179.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8597..8818)
                     /locus_tag="ACHKAU_003170"
     CDS             complement(8597..8818)
                     /locus_tag="ACHKAU_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5532180.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8881..9357)
                     /locus_tag="ACHKAU_003171"
     CDS             complement(8881..9357)
                     /locus_tag="ACHKAU_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5532181.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9373..9858)
                     /locus_tag="ACHKAU_003172"
     CDS             complement(9373..9858)
                     /locus_tag="ACHKAU_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5532182.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9949..10767)
                     /locus_tag="ACHKAU_003173"
     CDS             complement(9949..10767)
                     /locus_tag="ACHKAU_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5532183.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11107..12177)
                     /locus_tag="ACHKAU_003174"
     CDS             complement(11107..12177)
                     /locus_tag="ACHKAU_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO5532184.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALWEHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            complement(12174..13079)
                     /locus_tag="ACHKAU_003175"
     CDS             complement(12174..13079)
                     /locus_tag="ACHKAU_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO5532185.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(13076..15460)
                     /locus_tag="ACHKAU_003176"
     CDS             complement(13076..15460)
                     /locus_tag="ACHKAU_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO5532186.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININKAREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            complement(15571..18420)
                     /gene="cah"
                     /locus_tag="ACHKAU_003177"
     CDS             complement(15571..18420)
                     /gene="cah"
                     /locus_tag="ACHKAU_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO5532187.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            complement(18792..19664)
                     /locus_tag="ACHKAU_003178"
     CDS             complement(18792..19664)
                     /locus_tag="ACHKAU_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5532188.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            19993..20175
                     /locus_tag="ACHKAU_003179"
     CDS             19993..20175
                     /locus_tag="ACHKAU_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532189.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            complement(20404..20478)
                     /locus_tag="ACHKAU_003180"
                     /pseudo
     CDS             complement(20404..20478)
                     /locus_tag="ACHKAU_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(20475..20900)
                     /locus_tag="ACHKAU_003181"
     CDS             complement(20475..20900)
                     /locus_tag="ACHKAU_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5532190.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
BASE COUNT         4986 a         5530 c         5204 g         5264 t
ORIGIN      
        1 ttaagattaa cattttaata taacgagtta catttagtta catgtttaac acttgattta
       61 agatttgtaa tggctagatt gaaatcagat gtaatccatt agttttatat tttacccatt
      121 tagggctgat ttattactac acacagcagt gcaacatctg tcagtacttc tggtgcttct
      181 attttagagg cagctgtcag gtgtgcgatc aataaaaaaa gcggggtttc atcatgttta
      241 atgaagtcca tagtattcat ggtcatacat tattgttgat cactaaaccg tctttgcaag
      301 cgacagctct cttgcagcac cttaaacaat cgctggcaat tacaggaaaa ttacataata
      361 ttcaacgttc tctggacgat atatcttcag gctctattat tcttctggat atgatggaag
      421 cagataaaaa gcttatccat tattggcagg atactttgag caggaaaaac aacaatatca
      481 aaatattgtt gctaaatacg cctgaagatt acccgtaccg cgacattgaa aactggcctc
      541 atatcaacgg cgttttttat gccatggagg atcaagaacg tgttgtcaat gggttgcaag
      601 gcgtcctgcg cggcgaatgc tactttacgc aaaagcttgc cagttacctg attacacatt
      661 ctggtaacta tcgttataac agcacggaat cagccctcct tactcatcgg gaaaaagaga
      721 tcctgaataa gctgcgtatc ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca
      781 gcgaaaatac ggttaaaacg catctttata atcttttcaa gaagatagcc gtaaaaaacc
      841 ggacacaagc ggtttcctgg gcaaacgata acctcaggcg ataaagccat gaaacgttat
      901 ttacgctgga ttgtggcggc agaatttctg ttcgccgcag ggaatcttca cgccgttgag
      961 gtagaagtcc cgggattgtt aactgaccat actgtttcat ctattggcca tgatttttac
     1021 cgagccttta gtgataaatg ggaaagtgac tatacgggta acttaacgat taatgaaagg
     1081 cccagtgcac gatggggaag ctggatcact ataacggtca atcaggacgt tattttccag
     1141 acttttttat ttccgttgaa aagagacttc gagaaaactg tcgtctttgc actgattcaa
     1201 actgaagaag cactaaatcg tcgccagata aatcaggcgt tattaagtac gggcgatttg
     1261 gcgcatgatg aattctaaat aaaaaattgt tcggaggctg caatgcgtgt caaacatgca
     1321 gtagttctac tcatgcttat ttcgccatta agttgggctg gaaccatgac tttccagttc
     1381 cgtaatccaa actttggtgg taacccaaat aatggcgctt ttttattaaa tagcgctcag
     1441 gcccaaaact cttataaaga tccgagctat aacgatgact ttggtattga aacaccctca
     1501 gcgttagata actttactca ggccatccag tcacaaattt taggtgggct actgtcgaat
     1561 attaataccg gtaaaccggg ccgcatggtg accaacgatt atattgttga tattgctaac
     1621 cgcgatggtc aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc
     1681 caggtttcgg gtttacaaaa taactcaacc gatttttaag ccccagcttc ataaggaaaa
     1741 taatcatgca gcgcttattt cttttggttg ccgtcatgtt actgagcgga tgcttaaccg
     1801 ccccgcctaa agaagccgcc agaccgacat taatgcctcg agctcagagc tacaaagatt
     1861 tgacccatct gccagcgccg acgggtaaga tctttgtttc ggtatacaac attcaggacg
     1921 aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg
     1981 ccacggcaat gctggtcacg gcattgaaag attctcgctg gtttataccg ctggagcgcc
     2041 agggcttaca aaacctgctt aacgagcgca agattattcg tgcggcacaa gaaaacggca
     2101 cggttgccat taataaccga atcccgctgc aatctttaac ggcggcaaat attatggttg
     2161 aaggttcgat tatcggttat gaaagcaacg ttaaatcggg cggtgttggg gcaagatatt
     2221 ttgggatcgg tgcagacacg caataccagc tcgatcagat tgccgtgaac ctgcgcgtcg
     2281 tcaatgtgag taccggtgag atcctctcct cggtgaacac cagtaagacg atactttcct
     2341 atgaagttca ggccggggtt ttccgcttta ttgactacca gcgcttgctg gaaggggaag
     2401 tgggttacac ctcaaacgaa cccgttatgc tgtgtctgat gtcggctatc gaaacagggg
     2461 tcattttcct gattaatgat ggtatcgacc gtggtctgtg ggatttgcag aacaaagcag
     2521 aacggcagaa cgacattctg gtgaaatacc gccatatgtc ggttccaccg gaatcctgac
     2581 agataaaaaa gccggaatta tcatccggca ttttactgtt taagcgaaag cgtgagtgcc
     2641 cctcacgctt tatttttgat ttcctgctca cggtgagccg tctttgcact acgcgccgcc
     2701 agccacaggc cgctgttttt cattgcgtaa ccaaatacca gcccaagcgc cagcgaaggt
     2761 aacaccagtt tccagtcacc ctgcccggca aacgtcgcgc atgcgcctat aaacgtcccc
     2821 ggaacaaatg aaagcagcag ctgtttggcc tgaatacaca tcagaaacgc gacaataccg
     2881 gttatgacat aaccgagaat ttccagatgt ggtgccagtg cactaccgta aataatgacc
     2941 atcgcccaca ccacgccact aagcagggtt gcagcggaga tcgccagccc tttcagccca
     3001 ccttgcgggc aggcaaagta ggccgtacag cccaggaagc ccgcccagct tagtaagccc
     3061 agggaaacag ccacccatcc ccagataccg gagagaatgc ctgttgtgat tgcaatagag
     3121 agaagtatgt tcatgccgcg catgatagca gaaaacgcgg ccatgaatga gatcgagcac
     3181 acattttatg caacaacaaa ttatctgttg cagttatgag tgacgatgat cacatttact
     3241 cttcagaatc ttcttccagc tcgtcccaca ttgcactaat ggcatcacgg gttagcggtg
     3301 ccatggtgcg ccagaaaggc gtggttgcat gggcctccac tttgccgagg aacgcaccac
     3361 accagggtaa cagatactca ctgaacagtg tttccagtgc ttcgctctca tcttccgttg
     3421 actgatcttc cagccaggaa gccgcaagca gcaatgtgcc gatgtgatcg gctggcgtat
     3481 ccgccagtgg catccctcgt tcggaaagaa aagcgcgcac ttccgcttcc gttgcgtcct
     3541 caacccatgc gctacgatat ggcggcacgg cacattcatc gccgataaac aacgcattgt
     3601 aatcggcaga gacttgcgcc atatcacagc ttttctgtaa acgcgtcagt aactcatcct
     3661 gctccagtgg ccagttcgca gccagtttcc cctcacgaat cagggtaaac agcggcacca
     3721 gtaaaggatc ttgcggttgg cggtaataga gcgaacccag cacacgacag aggatagaaa
     3781 actcgttcat taatatattt ccattactag taattaaagg tctgcaaatt ccgcaatcgg
     3841 tgccataccg cgagattcaa ggaagttcag taagcggcgc ggcgaaacat tcaaaatgcg
     3901 ctctggcgga aaatctaccg cgtcgaggat tttaagacac tcttcaaatt cccccatggt
     3961 aaacgcggtg tgagaatccg agcctaatgc cacccaacct ccagcatcac gtaccgctgc
     4021 ggctaccgca cgacagttgt cttcactgcc cttacgtgag tgtaaaaatg aagaattatt
     4081 gatttccagc gccacctgat gtttcgcggc tgcttcagca acggctttca catctatttc
     4141 atatttggga tttccgggat ggcttattat atgcacattg ccgctggcga tagtggcgat
     4201 catcgcttgt gtattggtcg ctttgtcatg tggtgcaaaa accggctcat gaaaaccggc
     4261 aataatcaga tccagcgagt caaacatttt accgctgcaa tcaatttcac catcaacatt
     4321 tttaatgtta gcttcgatgc cgcgcaggat ccctacccca tcaaccactc gcggccagat
     4381 acgcatgtta atgaagtgcc agtgatgcgg agcatcttcc atatccgggc catgatcggt
     4441 gatcgcaaaa agtttaatgc ccttttgttt ggcctgggca atgtaatcac tcaatgtgct
     4501 atatgcatgt gtgctggcaa cggtatgcat atgaaggtcg acgggataca taactctctc
     4561 ctgtattcaa ttcccgccaa ggatagcagg aatcctgatg ctttattagt agccgcgtgc
     4621 gcggtcgact tgcccgcaga ccctctcccc tttttcgagc tgggcaatgg tgcgagaaat
     4681 gtactccaca gcttcagcgg gacgggtaat cgcggcgaca tgtggtgtta tcgtcacgcg
     4741 tggatgttgc cagagcggac tttcaggcgg taagggttca cgattaaaaa catccaacat
     4801 tgcgccttta actttgccgc tatccagcgc cgcgagcagg tcatcttcca caacatgaac
     4861 accacgcgcc aggttgagga gatacgcgcc atccggtaat ttttcgagta attgttgatt
     4921 aataatgccg acggtttcag gggtattcgg taacaaatta atcaataccc gacattggct
     4981 cagaaatgca gacagttctt cccgtccggc aaagctttgc acgccaggcc acgatttacg
     5041 ggttcgactc cagcaacgca gcggaaagcg ccaggtttgc agactctgag caactttact
     5101 gcccagtacg cctgcgccca aaatgccgat ggtaaaatct tcccgatgat attcaggcag
     5161 cggttgccaa tgcgaactat tttgctggat gcgataatcg tcaaaacgtc gaaaccaatg
     5221 cagcacctga ctgacagcat attcctgcat ttgctcgccc ataccggtat cttccaggcg
     5281 aaaaagtgga acagaagggt tcagcatttc agggtgtgcc tgtagcttgc tcaaaataga
     5341 atcaacaccg gccccgagtg cgaacaccgc tttaagatcg cgccctgcca gcatttcaac
     5401 aggaggatgc cagactaaag cataatcagc agagtcatta tctccgcttt tccacgctct
     5461 gactcttgcc tgaggaatag ctttgcgcag tgcctcaatc caccattggg tatcgaacgt
     5521 tgggtgataa aagatgatat ccatactgac tcccgaaaag cgttgtgcga aatttattcg
     5581 cacttatcgt tatgatctac aaaggccacc agcataacaa atccgtggtc ggtggcaaaa
     5641 aaagcagatt tcgcttatta aaaccacaca ttgattgaag tttgaataaa cgcgcgattt
     5701 tttcaaaaag tttgttgacc tcaggtcatg atttccctaa attagcgccc gttccagcaa
     5761 gacaggaacg acaatttggt gaggtgtccg agtggctgaa ggagcacgcc tggaaagtgt
     5821 gtatacggca acgtatcggg ggttcgaatc cccccctcac cgccagatta taagcgcatg
     5881 ttattgttta acatgcgctt ttttattttc ccttccagac agtatgccat cgggtatgcc
     5941 atctgaaacc ggtttatatg ccccgcgatc atatcttatc ggacaatttc cgatctttga
     6001 gcatactggc aaagctatac tgccagcata tcaggatagt gttttgcgat aatatcattc
     6061 agtctgtcaa ccagttctgg ttttctgaac gtgatatgcg cggagccctt cttaaagtat
     6121 ctgatgctga acatctcatc ttcatagctc tctttaccct gaacagactg aatgtgatca
     6181 tccagacgaa gggtgatgtt tccccggttg tcggggattg gtttaccgct gaacaggtgc
     6241 aacattcttt ccagatcggc gagtcggtca gcctgttgtc cggtgataag atgaaatccc
     6301 caccggtccc atctcaccag attgttgacg ataattttac tgccaaattt acaggggcaa
     6361 ttggttttgt aattccagtt cagccctctg aagacgttga tcacccctcg ctcaaacacc
     6421 tcatccttat tctggtgcag ttgttcaaat gtgctcagaa tgttcgcttc gctgatttcc
     6481 ggaaaattat cgtactccag tgaccggtac catgtatcac gggcctgcgt atccattaat
     6541 gacagcatcc cggaacgttg catcaggtca cgccagattt cgcgatcaag attccgggta
     6601 atggctttca ttgctgtttc gggtttctcc atcagccagc agccgcagcg aaaatcctgt
     6661 ttcatggccc agtccggggc agttttacct ccgatgcttt ccgtcagcac cgagatttca
     6721 gccagttgtc ggataagtgt gtcgatttgc aacagtgtac tctttcgtac agccagaatg
     6781 tggcgaatgc tggttgagca gataacgtcc gtatgcccgt tgaccacttc cggacgggag
     6841 attgtgatat ctgacataat cgatattcct ttttgcagat aaatgaaaac gccagccctg
     6901 tgcaggctgg cgtcagagga atgaagtctg gtgagtgagc ttcaccggtt ctggtgcaga
     6961 agctgcagat gacgcagggt acggacatcc tcccgggcct gttcgataca gggcaacagt
     7021 gctgctgaat ccgttttatc ctcatcgttg tcaaagataa ttcccgatcc gcagtcgata
     7081 ttgtcctgca gccacgcaat cagaatatcc agcgctgttt ccgtggttaa tgatttcatg
     7141 ttgttcctgg ttaccagtcg aaagtgggta aacccggcag gcattcagcg ctggcatcca
     7201 gatgaatgag actgacgcca taacgccgga tgagtgtgac gaccagacga cggaacgttt
     7261 ttgacagccc caggcgttta agccgcagaa ccgggtatga ccacgcatcg gtacgtaaca
     7321 gatatccgct acctgtaaaa tgaatccatt ctgattcacc gaagtcactg gtctggtgtg
     7381 acagcgagta caaccaggcg ttgtccgttt ccgtgatatg tgcggtactg cagcgtatgc
     7441 cggtaagggt agcaaatggt ggcgtgcatt ctgctgaggt ctggtcagat tcatcaacaa
     7501 cacgaaggga gtagccgtta tcgcagacca tgttaatcag ttcagcgagc tcaataccat
     7561 cgacgtcaac gacaatgcgc cccatgttca gtgcctgtac gttaacgctg tcggcttcca
     7621 gtgtcagggc cagtttcatc atttcgcctc cggatactta cccaaggtaa tgttatttac
     7681 cgttctgtaa ttgtcgcggg tcatcaggcc ggtcgcccgg cgagcccgga ggatatcaat
     7741 actgtttatt aactgagagc ggggacaggt gctgaatccc ggctggtcgg tacgcaccag
     7801 cgcgtatttt tccacgagaa aattaactgc atcacacagt gaaatgcctg cctcaatatg
     7861 ctgctcaatc acacgttcat cggcaaacgg tgtgtcattc agtgtgaggc cataatgctg
     7921 gtccagcagt cggatgagca gtgtctgcca gatggtgacg ggagacgggc agcgagatac
     7981 ctcccgtaca tgtgtgtcgg ataatgtttt catcttaagt tctctgtaac tggtagtgct
     8041 aaaggtgaga gcttcggggt tatgaggtgg ttgcgagtgc tggtgtcgga taaacagcca
     8101 gataaacgta accacaggag ccgagggtgt cggcttcgca ggtcagccct tttgcattca
     8161 gcgtgacggt atgctgatat cggggattca gttcaccgct ggtgagcatg agttccagtt
     8221 gtttcatcag cagcggaaag gcctggtcca gatggtatgc atctgcgtcg ctgaagcggc
     8281 ctctgatacc ggcgcggtcg gcaaggtaat gcaggcggtt accctcttgc accagacgag
     8341 cgccaaaaca gggcctcact gtgcagggaa gcccccacca ggggcggtcg tgattgtcat
     8401 cgggatgagt tatcccggag agtttgtctg acacgataaa atcctctctg aaaagtggtt
     8461 aagaatggtt taacgatgac aggtcagccg gcgcgctgac cggtttcggt gaagatgtat
     8521 gcattgatga gaatggcctc atccacagcg gcgtctgagg tcatctggtc gtactcgttt
     8581 cccgggtgac ggaacatcag ttcagggtga cactcatcag ccggacatac gggccaaaac
     8641 tgtccttacg gcgttctgca aacacggcaa gaacgccggg aatatcctgc acttcacgac
     8701 cagtatacgc ttcagcactg ccgtgccagc gatacttacc ggtacagaac ggaaaaagac
     8761 gggatgtcgg atgttgttgg tgaatacgca tggcttcacc acgggtgata attttcataa
     8821 tgggatacct ctgaagacag aagataaaag tgaaaacagg tgtgatgtgg ttgtgaaggg
     8881 ttaaagcaga ccatgttcgg caaaggagaa aacctggttg ccaccgacta tcagatggtc
     8941 cggcacccgg atatccacca ggcccagtgc ctgtaccaga cgttcggtga taagccggtc
     9001 tgccttactg ggtgtgactt caccggacgg gtgattgtgc gccagcacca cggcagcggc
     9061 attgtggtac agggcgcgtt taatcacttc ccggggatga acttccgtac ggttgatggt
     9121 gccggtgaag agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg
     9181 gaactcttcc cgctccagtc ccgccatgtt cagaatcagc cattcccgtg ccgcacgggt
     9241 ggaggtgaag gccacgccgg gttcatgaag atggcggtcc agggtttgca gggcccgcag
     9301 aatgagactg cgctcgccgg gcgtcatctc tccgggcaga aaggaaagct gctgcattgt
     9361 ccctgcctcc attcagtcaa tgatgcgcat aatggcgctg cattccggat gctgcagggc
     9421 gtaatcccgc agccggtaat agtggaccgt catggcataa cactccgtac gacaggcatg
     9481 atgactgtac gtcatcagac aggcagcaat accggcggct tccgggctca tttcagcgcg
     9541 gttgccgttc atggcattga acagtaccca tgtttcgtca tcatcgtcat ccggttcggg
     9601 tgccataaat gccccgccgt tgttcagggt gtacagattc cagataccac cgcagtagtc
     9661 ttcgcaaaga cggtccatcc agccgaagac acggggctcc agggtcaccc actgtggaat
     9721 gaggccaaaa tgctgcggcc agaagctgat gcgctgttca tcagggacgg gagtggcaac
     9781 cagctgaggt gggttattcc ctgatgcagt ggttacggaa acagaaggtg tggtggaatt
     9841 atgcgaaacg gttgtcatga ggttattcct tataaaaagt aaatgaatgg aagaagcccc
     9901 ggggcaggaa cagatgtgag tcaggactgc actttcagtg aaacggcatc agcgcatgct
     9961 ctccagcagc gtttctgcca tcacccacaa tgcgcgattg agcttaatgt cggtatcaat
    10021 gctgtgaatg gcccgggtat ggatacgttt tccttttgca ctgcgaccgg aaatcccgcc
    10081 tttcagcata ttctcctgga tggtctgata agcactccac aggtccttac cgtaatcctc
    10141 ccggcgtcgt ggtgtcagaa tgtcggcggt ggtgacgggc tgatgttcgt caccataacg
    10201 gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt ggcggcagga ccagcgactg
    10261 catggcatca cgcttctcct ctatccggtc aaaaacgccc accacctcgt aagccccttc
    10321 aatgactctg tccaccacat ttccccggtg tggaacacgc aattctccca gagactgacc
    10381 gcagaccagt ccattggtac aaatggcacg aaaatatccg ggtaacatct ggtagctgga
    10441 tgaaccgtca tgggagttga gcagaataat ttcagggaca tgctgaccgg ttatctgtcc
    10501 ggcccgccgc agacgcagca tgtgttttgt gtattcccgg cggctctggt cgcgcacgcg
    10561 ggtctggcag gcaaagaacg gctgaaagcc ttcctgctgc agattttcca ggacggtgat
    10621 agtgggaatg tacgcatagt gttcactgcg agaggtatgt cggttttccc caaaaatact
    10681 cggtacatgg cgcatcagtt cttcgtgtgt cagcggacga tcacgacgta tctggttcgc
    10741 ataaccaaaa cgactggcta atctcatgat ttgctcctta tgaatgatta agcgatataa
    10801 atgaaaaagc cgcgtctccc ggagaagacg cggcctgatg ggtgaaatga atgaggttta
    10861 ttgtctgaga agcccttcac tggcgaacca cgtattaagc tgtattccgg catcgccagt
    10921 gcaactgacc ttcagcattg cggataaccc gccggaaata tgttcactgg ttgtttttgt
    10981 atcttccccc aaaattaaat aaattcatct taaccatcac ccagataata tatatctgaa
    11041 aatattttca ataacaccag acaggaaaaa caccaccaca ggcggtggcg tttctttggg
    11101 gcgtctttat ttaatccggg ccatcgcgtt tctcagtgcc aaatctgcct cttccgttat
    11161 atccaggctt ttctgcaata ccgcctctga ccgggtcagt gcctgacgtg ctgccagcgg
    11221 tttccggata tgctccatcg ccagcatcgt gtcttcatag ccctgctgac gccaggcgtc
    11281 ggtatgtgcc gatgtaaaac tgagcagacc gccgctgttg ccaccatcac ctgcatattt
    11341 cagccttttc cggggacgga tctcaaggat tgtggtgtcc ggaaaagcct ggcgatccca
    11401 taaagaaccg tcactcagat gcgtcacaat caccatattg catccggcat cgaccagagg
    11461 cgtcacaggg gtatttccct gcatatttcg ccatcctccc ataccaccat caccgaacat
    11521 tgtcccctga acctcacggg gacggaacag caggggcagc gcggctgacg caagcagagc
    11581 ctctttctgc tgtccgcggg gcaggctctg gatatgctga aaaacggcgt ttttcgtggt
    11641 tccgacaccc agttcagcac gaatgcagtc aataatatcc tgcatgcccc cttctgtggg
    11701 gtacagcgac acatacagcg gtagcccgtc tgccagagca tcagtatcaa gataatgatc
    11761 catcagcgct gtcaggggct catctgacaa caacggctga gccatcagat tgtcaaaccc
    11821 gttgattgtc gatatatggc gaagaagcgt cgtaagcagt gctcctgcac gtccggggat
    11881 ctggcagagg ttcattgcct ggaacaattt tttcagcaat gaaaagtaaa ccaatctgtt
    11941 taccgacagc acctgattat tccccagatg ctcccagagc gcctccaggc gtacggcagc
    12001 ttctgacaga tcgggagagg ccgcgataat ggcaccattg aatgcgccaa tgctggtccc
    12061 tgaaaccatg ctgatctgtg taccacactc tgccagtgcc ttaaccactc ccgcctgata
    12121 agcgcccacc gccccgccac cggacagcac cagccccgtt ttcatttctg tactcatgca
    12181 gcccccttct gaaatatttc atcgaccata ctttcagaga ggcgttctgc agagcagagg
    12241 cgaggcatgc gaaccggtaa gtgttctgca gcctgcgtgg ttgaccagat caccggatgc
    12301 tgctcttcac tgcaccagtc tcccagccag ttaatcgcgt catgccactc gttatctgcc
    12361 tgctcccggc cctgaccacc aagccagtcc agagtgttca gacgcacggt tgcactaccg
    12421 ccactctcct gtgccatctg cgttaatgct ttatgtctga gaatatccag tagctggtta
    12481 acctggcctg tttcgccctg acgtatcaca tcaccaaacg cgccccagcg aagctcttca
    12541 agtgccacat aacaacgtcc ggcaggggaa aatgacgcat atcgcccggc gctccaccat
    12601 gagaatatct gctcaagatg taactgcgcc cgctgaacct ggtcaacacg gtggagtttt
    12661 tcttcaagat gattcagttt atggtgaacc tgatcggcca gggtgagtaa ctgctcccgc
    12721 gtatctgcgg aataatgcgc cagcctggct gtatcactga ccagtgaact gaccctgtcg
    12781 tttacccggg taatcgcgta gtttgtggtg gcaagtgacg ttgtcatctc cgtcagccag
    12841 cgcagggacg cctcaacgcc ctgtgcaagc gacgcattga tggcattctg ccgtgcagcg
    12901 ccttcgccgg tcagcccctc ttttaaacgc cggaagaacg tgcgctctcc ggccgtgcgg
    12961 atatgctcct gtgcaacatc gattccgttt gcgaaatcaa ccacaaattt ttcaggaaga
    13021 caggtgagta cctgacatac ccggttatca gcaatttgtt gaataaatgg tgaagtcaca
    13081 gcattgtctc cagctcttcc ctcagcgcct tactgtcagt aatgagctca ggaacccttt
    13141 catttaatgc ctgaagtcgc ccggtaagcc gttcctgcgc ctgctgatcg cgtttatgat
    13201 cttcagagct ctgaatcagc gtgttacgca gttgttcaac tttctccctg aatgcgcaga
    13261 aaaaggcatc gatttcctgg cggacgggct ggttaatatc ctgctcaatg gatgcctgca
    13321 gctccccgaa atatgcccgg gtcagtgaca taacttcctc tctggccttg ttgatattga
    13381 tcacgctgcg actcacatcc tcgtcatagt tttcccagcc caggtcactg gtgccaaacc
    13441 atcgacaaac ggttccccat acgccactct gccgacggcg acgggtgacg gtctccgttc
    13501 tttcctccat aagcgcactc agatttattt ttaccgcgag ccgggtctgt aactggctga
    13561 ctgccggaaa actgattttt acggtaaagc cctcatcctc caggcgggca ttgatgcgac
    13621 cggcaatgtt ttctgtggcg tacagggccg tgccacgaaa acctgtttca atcccgctga
    13681 cgattttctc cagctcaggc cggaactgtg cttcagcctc acggtgcagg ctggtcacgg
    13741 tcgattcgat ttgcgtcatc agttcaaggg ctgccctgcg atcactgaat tttatttcgg
    13801 ggctgtccgg atcaaagtcc cgcatcctgt catgctgatt tccggttccg aaaatatcgt
    13861 gaaagaaagc gaatgcgttt ctctcccggg gctgcgctga attttcttcc tccagcattt
    13921 gctgttcttt gcgtttaccg cttctgaaat agtcgttcag ctcctcctcc agtgaggcac
    13981 agaccttcgt cagtaattcc ccggttttgg tattgatgtc ctccatggcc attctgacat
    14041 caccggtcac ctgattctga cattcctcaa tctcctggat atccgccagc aggctggtaa
    14101 tatgcgcctg tagtgactga atgctttgct gcagcccctg atgacgtaat gacagatatt
    14161 cgctgacatt ttctgcattc tgcatcagtt ttgcggcggc agagtcaacc gccagtgccg
    14221 ccgctctgga atgggagctc tgtatgactt ccgttatcag ttgatcgatc agagatatat
    14281 tccagagttt ttctgccttt ttatttacct tagtgctgtc tttccagtcg tcctcttcca
    14341 ccataggtcc gaaagcttcc ctgataaaat catcgaccca ggcttcatca accggaagcg
    14401 caccgttttt tcgtaactca tgaagcgcac ggttagccag gtacgcctga cgtgatgagc
    14461 cgggataaac gcgggaggcg ggcagcacat cgctgttcag cattgctggc accttctggc
    14521 gtacggcatc cgcaccatca ccattgcgat ctttttcatc gaatttattg accagcacaa
    14581 acagacgacc ggctgagacg tcagcaatgg cattaagctc tttacgaacc tcctcatcgg
    14641 ctttcgagtt catctgggtg taatccatca ccgccagcac cgcagaggct ttctgtagtt
    14701 gatcccgcat catcatttcc atctgtggtt gtccggcttc attagggccg ggggtatcca
    14761 gcagtgtgag tgttccctga catgcatcca ttcccaccag gtggctgaac gccacgtcga
    14821 tcaccggtag tttctgcact tctgcgtatt cgtcaaaagg aaattcagtc cccattgccg
    14881 cagcaagacg aaccaggtca tttaacgatg ccagtccggt aaagatttcg tcttccccgt
    14941 ggtattcatt tttaagccag acatcatccg ccagaatatc cagtagttcg cgggtatccc
    15001 cggtttgctg cagggactgt gcgacttgct gccctgccgg ggtggcgagt ttttcctgca
    15061 gtgtgattaa taaattgcgg acgggctgaa tatgttccag atggaggacc ggttccgttt
    15121 ttccgggaac gtggcggatc aatgtcggta ccgaggtcat ggggcggtta cggttgggca
    15181 gaatttcctg ccccacaatg gcgttgatgg tggttgattt gcccgccttc atcgtgccga
    15241 ccacagccag caccatctcc ctgcgggcgg ttttgatttg ttcgccctcc agctcctcaa
    15301 ttcttttcag cgctttccgt ttatcaaaaa gcagcccgtt ttcgttctta ctgtcagata
    15361 acacatccgg ctcatcaacc atttgccgga gcagattgat attcagttgc aaaagccggt
    15421 cggcttcatc acaaagcagg gcgatgttct tttcgtgcat cattcacctt tataaattca
    15481 ataattcaga actgcggtga acagcaatga gcgtcctttc ccattactgc atgaattttc
    15541 ctgaaatcgt atttccggga taccgtataa tcagaaagtc atattcagcg tagcctgacc
    15601 gttatagcct tcagcgctgc tgccgctgac gctgtgggca taaccggcct gaacgcccag
    15661 ggtgatattt tcccggatac gggcttccag tccggcctgc aggtccagtg acgtgccatt
    15721 ccgggacggt gagaacgtca tgttactgcc ggctgcggct gtccccatgc tcatgtcacc
    15781 ccgggagctg aaggtgcgga taacagaagg ctgtacccac cagttcaccg gcagttcact
    15841 cacactgtgt tttgcactgt cgcgcagggt gtcacgggat gaggtgcctt caccaaaggt
    15901 catatcgttg tggctgccca gacggaaacc ggcacgcaca tgttgtgcac tgccatgccc
    15961 gaacttcaca taaccggcgt tatcctggcc gtcatccagg gagagtccct gccaggtgta
    16021 ctgcagttgt ggctccagca tcagattgtc agtgatactg aagggcagac cggtttccag
    16081 tgagcccagc cagccccagc cccgggcgcg gaagtcgtta ttgtccgatg acgctttcat
    16141 gctgtgacgg gttccctggg ccacaatgtc agcccacagg ccggaggatg tgtgtaccag
    16201 attcaggtat ccgcccagac tgccggcatc atcccggacc gtgccggcgc gggaaccgtc
    16261 atcatcctta acatcaacgg aagaatggcc tgcagcacca tacacccctg tcgtcagaga
    16321 cataccggca acctctgttc tgagcaggtc accctccaga cggacgaagc catagctgcc
    16381 gctgctttcc ggcgtggctc cacgggcaat accgccgttg ttatcgtgac cgagatgacc
    16441 gccctgaatg ctgagacgga cgctgttatt ttcaccgttt acaccggtct gatggctgcg
    16501 ggagcctgcc agaatccggt catagtccat tgcctgtgtc aacatggatg tatacagggg
    16561 gacttcagca cgataagcat tttcactgcg caggtaccag tcttcatcgc tgtcacggtt
    16621 cagggtgtag ttaaaggcgc cggcctgaag cgggcgactc agggcaaacg caccttcttc
    16681 tgtggtggcg ccattctgtg catccacaac ccggataccc tgtccggtgg ttgccacccc
    16741 gaggttgctg tttccgacat ttgtaaacgc aagccaggtt ttgccggttg cctgaccacc
    16801 attaatcacc agctggtcag aggcattgct gccatcaagg cgaacacgca tattgatggt
    16861 gccgccctgg ccggtgaggt tcgtggtggt cagtttatgg aacgttaccg gggagttact
    16921 ttttgcaaca gcacggctca gcgcggcatt cggtgtcgtc tgattatcaa agcgaatctc
    16981 ccctgcgtta acaatatcgc cggtactgac cacatcacca ttcagtacca tactggcgcc
    17041 tttactgagc tgtgttctgc cactcagact tcccccggca gccagcgtca gtgttccacg
    17101 atgtccgaca gtggtgttgc cagcctgtcc cccggcatta accgtaaagc tgccgccatt
    17161 ttccagcagc aggccgctgg cctgaccgga tgtgccatca atactgaact taccgctggc
    17221 attggtcccc tcaacagtgg caccactgtc tgcaatcagg gcaccaccgg atgttatggt
    17281 gacacttgtc gccttaccgc cggcagacac tgccagggta ccgccgtcat ccactagggt
    17341 attctgtgct gaatggctct ccagtacatc cagacgaccg ccggattcca gaacaacacc
    17401 gtcagcctta ccgttttcca ccgtgaaatt ccccagacgg tttgtgccga tgacagttgc
    17461 agcagtactg gtaaccagtg caccgcccgt gttctgggtg actgcagtgg cttccccgcc
    17521 ggcatgtacc ctcagttcac cgttctgatt tatggtggtg ttaccggcag cgccacctgc
    17581 cttaacaacc tgccagcccc cctcgttaat taccgtgttc agcgcaagtc cgtccctgtg
    17641 cacatactgg taaccgccat tcagtgtggt attcagggcc cttccgtgta ccgactggtc
    17701 accacctgcg taaatgagag tgtcacgggc tagcccggaa aataagataa tctgccgtcc
    17761 atttttgttg atggtggtgg attctgctgt tccctggacc ttctgacccg tatacgagtt
    17821 gtcagaatca gggccgcctt ctgcgccggt gttgatgatg gttcctgttg ccagcccgcc
    17881 acttttaacg ctctggtaac cgtcgcggtt gataattgta ccggtggcaa ccccgccctc
    17941 atgcacccac tgctcgcctc tgttattcag tgtggtgttc accgccagtc cgttcaggct
    18001 ctgtcccccg ccgtcacgaa taaccgtatc actggctgtt cccccctcca gcacgacctg
    18061 acgaccattt gtggtgacag tggtgtttcc ggctatcccg ccattctgta tccattgccc
    18121 accggtgttt tcttcactgt ccggccccag ttccagcccg gtactgatgg tcattccgtt
    18181 ggccgtaccg aagacaatct ggttgtcatg atttgtcagt gttccatcgt tcacggtttc
    18241 tcccgcctgt acaaccttgt cagcagccag tgccgggact gatgtgacag cagcaagaga
    18301 cagcgcaacc gccacaccgg cgcgttttcc ccgtgagcgg gccagttcgg aggccaccac
    18361 cagggtgccc gtaatgtgat tccataccag cctgtagctg gtgttcagat gtcgtttcat
    18421 cagcttttcc ttacagaggg tgaataaaaa agccggtacc cacaaaagtg gataccggca
    18481 ggtacaaaac agagttatcg ggcacagcga tacgtcccgg gcgtaaacgc tctccccctt
    18541 catcagacag agaagtatca gtctgttgtg tgcaattccg ttctgcatga tgaagagatt
    18601 cagctgcctt ttcgcaggcc attaaccact ataaacgatc gatataaacg atcgatacct
    18661 gttttatcga tcgttttatt ctattaacag tagagcagtc acgcaagaga aacaggaggg
    18721 gcatgaggtg tttgtatcat gttttcagtt ggttaacgca atgtaatcag acatttcccc
    18781 tggcagactg ttcagaagaa aatccagttc cataccgcgc gggcaacaga gaccaccgtg
    18841 tcacggacgg cacgcagaac tgtgcgtgca acagaggcaa cacagacgct ctccaccgtg
    18901 tcaaatatcc ggtccaccgc accggtaaac tgttcacggg cctgcccccg gacagattcc
    18961 gtgcacagct cgtcctgcag gcgggtcatc aggggactgg cggcatggtc gggaagcgct
    19021 gtcatgagcg cactgaccag cgtatccagt tcccagccgg tgcgggccga tacggcaaca
    19081 accggatgta cgggccggaa cagccggaat accgcatctg ttttttcgcg aatgttctgt
    19141 tcctgtgcgg gagaaggctg aatacctgcc atatcccatt catggcaggg ctctgttttg
    19201 tcggcctgcg tcaccacaaa cagcacctgc tgatgtcccc ggtgcagtat gtgtcgccag
    19261 aaatactcat ccacagacag ggcacggtca tcggctttaa tcagccacag taccaagtcc
    19321 agttcaggca gaatgtcacg gtacagggct tcatactctg catccctgtc ccggctctcg
    19381 cccaccccgg gcaggtcagt gataaccatg ctgtgaccat ggccactcag acggaagcgc
    19441 cgcacttccc gggtgccggc gtgaacatca ctgaccgggg tgacctcccc ctgaaacagt
    19501 gcattacaga gtgaggattt accggccccg cttttaccca taatgccaat cacgggttcg
    19561 tgactggtga gtttgcgcag atgttccagg atgtgacggg aaagcgagta aggcagggag
    19621 gagagcggtt tttcaattgc ctcaatggca tcagacggat tcatacatgt attcccggtg
    19681 aaaaaagaca aaaccccgcg caccggaaag gtggcggggg attcacaacg ataatatctg
    19741 tctgaaatta tttaggatgc aggtatcttc tgataatcat acatcccgaa ttttgttctt
    19801 aatagttgag tagtggtatt tggtttccct gagagaatat aaaggtgaat catgctgcat
    19861 tgagtttgat tcaatttgta ctgagacaag tatcttcatg attataatca aaagttgggt
    19921 ggattggtct ggcgctcatc attttttcga ataaactcac taattattgc cccccccaaa
    19981 ggagatgatt tcatgtataa ttgtggtgaa tcatattcag aatacgacat atcattatct
    20041 gacgatgaaa actatgacga tgactattat cgtgaacgcc cgtgtccggg agaagttttc
    20101 ggctatgata atactgagga taatgatgag gcggcatctg attatgcatt aaaaataaat
    20161 gaaaactctg attaatacgg gggtagctga taataagaca atcaatgaca gatattaatg
    20221 aataaacata atcaggcgct caatacttgg tttaatctgg gcgcctgatt aaaatctgag
    20281 gaaacatata ataattttca tcaaaatagt atttatgtac taactaagct ggatttgaac
    20341 ctctatttct gggtatttgt tatcacttaa tccattactg ctcctccttc agcatatgtc
    20401 gtatccgttg aaggatttac gcatatcggt aacgtcggca acgagccaga tgcgggtacc
    20461 tgacgggagt gagatcatcg tcccctcccg gtcagttcac ggatcaacag cgtgagcagc
    20521 tctggcgatg gattttccag cgtcatgtta ccgtgacgga actccacctt gcaggagccg
    20581 gcactgacac tggtctgagt ggaagtggat aaagacggcg caatggccgc caccggttct
    20641 ttctgctcat ccggcgttat ttctacaggt aataattcaa cgccagtgtc agaagaggtc
    20701 gttacctgaa gacgccgtga tatacgccct tcgttctgcc agagcctgag ccatttgaac
    20761 aggaggttat cattgatatc gtgttcccgg gcaatacggg caacagaggc tcctggttgt
    20821 gatgccagtt ccaccatacg aagtttgaat tcattcgaat agtttttacg aggttctttt
    20881 cgccagtcct gtaattccat acttagatgt ccgtctatat cagatgggcg tctaagttac
    20941 caattttcgt ctgatggcta catacggcgg tcagtttacg ctta
//
LOCUS       DAZFJF010000078        20619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFJF010000078 DAZFJF010000000
VERSION     DAZFJF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.078"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(15..1664)
                     /gene="actP"
                     /locus_tag="ACHKAU_003182"
     CDS             complement(15..1664)
                     /gene="actP"
                     /locus_tag="ACHKAU_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO5532191.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1661..1975)
                     /gene="yjcH"
                     /locus_tag="ACHKAU_003183"
     CDS             complement(1661..1975)
                     /gene="yjcH"
                     /locus_tag="ACHKAU_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO5532192.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2176..4134)
                     /gene="acs"
                     /locus_tag="ACHKAU_003184"
     CDS             complement(2176..4134)
                     /gene="acs"
                     /locus_tag="ACHKAU_003184"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO5532193.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4526..5962
                     /gene="nrfA"
                     /locus_tag="ACHKAU_003185"
     CDS             4526..5962
                     /gene="nrfA"
                     /locus_tag="ACHKAU_003185"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO5532194.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKLQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6007..6573
                     /gene="nrfB"
                     /locus_tag="ACHKAU_003186"
     CDS             6007..6573
                     /gene="nrfB"
                     /locus_tag="ACHKAU_003186"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO5532195.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6570..7241
                     /gene="nrfC"
                     /locus_tag="ACHKAU_003187"
     CDS             6570..7241
                     /gene="nrfC"
                     /locus_tag="ACHKAU_003187"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO5532196.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7238..8194
                     /gene="nrfD"
                     /locus_tag="ACHKAU_003188"
     CDS             7238..8194
                     /gene="nrfD"
                     /locus_tag="ACHKAU_003188"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO5532197.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8214..9932
                     /gene="nrfE"
                     /locus_tag="ACHKAU_003189"
     CDS             8214..9932
                     /gene="nrfE"
                     /locus_tag="ACHKAU_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO5532198.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9925..10308
                     /gene="nrfF"
                     /locus_tag="ACHKAU_003190"
     CDS             9925..10308
                     /gene="nrfF"
                     /locus_tag="ACHKAU_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO5532199.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10305..10901
                     /gene="nrfG"
                     /locus_tag="ACHKAU_003191"
     CDS             10305..10901
                     /gene="nrfG"
                     /locus_tag="ACHKAU_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO5532200.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11243..12556
                     /gene="gltP"
                     /locus_tag="ACHKAU_003192"
     CDS             11243..12556
                     /gene="gltP"
                     /locus_tag="ACHKAU_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO5532201.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12634..13323)
                     /gene="yjcO"
                     /locus_tag="ACHKAU_003193"
     CDS             complement(12634..13323)
                     /gene="yjcO"
                     /locus_tag="ACHKAU_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO5532202.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13417..15564)
                     /gene="fdhF"
                     /locus_tag="ACHKAU_003194"
     CDS             complement(13417..15564)
                     /gene="fdhF"
                     /locus_tag="ACHKAU_003194"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15145..15147),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO5532203.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15762..17228)
                     /gene="mdtP"
                     /locus_tag="ACHKAU_003195"
     CDS             complement(15762..17228)
                     /gene="mdtP"
                     /locus_tag="ACHKAU_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO5532204.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17225..19276)
                     /gene="mdtO"
                     /locus_tag="ACHKAU_003196"
     CDS             complement(17225..19276)
                     /gene="mdtO"
                     /locus_tag="ACHKAU_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO5532205.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19276..20307)
                     /gene="mdtN"
                     /locus_tag="ACHKAU_003197"
     CDS             complement(19276..20307)
                     /gene="mdtN"
                     /locus_tag="ACHKAU_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO5532206.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20326..20601)
                     /gene="ytcA"
                     /locus_tag="ACHKAU_003198"
     CDS             complement(20326..20601)
                     /gene="ytcA"
                     /locus_tag="ACHKAU_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO5532207.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4633 a         5486 c         5659 g         4841 t
ORIGIN      
        1 gggaagggga gagattaatg tgcgcggcct tgctcaacgc caaagccggt ctgggagcgg
       61 ataaactgtg cacggaacag ttcacgctcg cgcgcacctt ccgctgagtt atcggttgcc
      121 gagaagaacc agatgccgag gaatgccacg ctgatagaga acagcgccgg gtattcatac
      181 gggaagatgg ctttttcgtg accaaggatc tgtacccaaa tcgtcgggcc gaggatcatc
      241 agtaccactg cggtaatcag ccccagccag ccacccatca tcgcgccacg agtggtcagt
      301 ttcgaccagt acatagaaag cagaatgatc gggaagttac agctcgctgc gatggcaaac
      361 gccaggccta ccatgaaggc gatgttctga ttctcaaaca gcacgccgag gataatggca
      421 atcacaccta aaatcagcac ggtgattttc gatacccgca gttcttcacg ttcggtcgcg
      481 ccttttttga agacgttagc gtacaggtca tgcgaaaccg ctgatgcgcc cgccagcgtc
      541 agaccagcaa ccaccgcaag aatggtggca aaagcaaccg ctgaaataaa accgaggaac
      601 aggttgccgc ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga
      661 tggcccgccg cgtctttata ttccggattc gcaccaacca gcatgatcgc gccgaagccg
      721 ataataaagg tcagaatata gaagtagccc ataaacccgg tggcgtagaa cacgctctta
      781 cgtgcttcgc gggcatcgct gactgtaaag aagcgcatca gaatgtgcgg caagcccgcc
      841 gtaccaaaca tcagtcccag acccagagag agcgcggaga tcggatcttt caccagcccg
      901 cccggcttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcact gaacagattg
      961 ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc
     1021 aacagcacgg ctttgataat ctgtacccaa gtggtcgcca gcatgccgcc gaacaggacg
     1081 tacatcatca tcagcacgcc gaccagcacc accgcaatgt gatagttaag gccaaacagc
     1141 agctcgatca gtttacctgc gcccaccatc tgggcgataa ggtaaagcgc caccaccacc
     1201 agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacggta agaggccaca
     1261 tcggcaaagg tgtagcgccc caggttacgc agacgttcgg caatcaggaa caaaatgatc
     1321 ggccagccca ccaggaagcc cagcgagtaa attaagccgt catagccgga ggtaaacacc
     1381 agcgcggaga tccccaagaa tgaggcggcg gacatatagt ccccggcaat cgccagcccg
     1441 ttctggaagc cagtgatatt gccgcctgcg gtgtagtagt cgctacgaga acgtacgcgt
     1501 tttgatgccc agtaggtaat gccgagcgta aacacgacga aaatcaggaa cataataatc
     1561 gcctgccagt tcgttggctg gcgctctacg gccccgctaa tagcatccgc ggcgttagct
     1621 gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat
     1681 gcaggacttc attattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccgg
     1741 tgagaacaaa ggagatcaca atcactccaa caccaattgg aataccgcgt gtgacgctgg
     1801 tgttcggatt cagcggcgtg cccagccagc cgggcgcgaa ggcgatcagt aaaataaagc
     1861 cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga
     1921 ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatcgtacca ttcatcacag
     1981 attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa
     2041 cgctcgggta acccttgccg aatgtaggcc ggataaggcg ttatacgccg catccggcaa
     2101 tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta caaaccgtta ccgactcgca
     2161 tccggcaatt gtgggttacg atggcatcgc gatagcctgc ttctcttcaa gcagcttctc
     2221 gactacgcca ggatcggcaa gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc
     2281 aattttgcgc agaatacggc gcataatttt gccggagcgg gttttaggca gggagtcggt
     2341 ccagtgcagc acgtctggcg tcgccagcgg gccaatctct ttacgcaccc agttgcggac
     2401 ttctgcgtac agttctggtg acggttcctc cccgtgatta agcgtgacgt aggcgtagat
     2461 cgcctgacct ttaatattgt gcggaatacc tactacggcg gcttcggcaa tcttcggatg
     2521 cgccaccagc gccgactcaa tctctgccgt ccccagacgg tgaccggaga cgttcagcac
     2581 gtcgtccaca cgcccggtga tccagtaata gccatcttca tcgcgacgcg cgccgtcgcc
     2641 gctgaaatac atatttttga aggtggagaa gtaggtctgt tcaaaacgtt cgtgatcgcc
     2701 aaacagcgta cgcgcctgac ccggccagga gtcggtgatc accaggctgc cttcggtagc
     2761 gccttccagc gggttacctt cgttatcgac cagcgccggt tgcacgccga agaacggacg
     2821 tgttgccgaa ccggctttca gctcggtagc gccaggcagc ggcgtgatca tgaaaccgcc
     2881 agtttcggtc tgccaccagg tatcgaccac cggacatttc tcgttgccga tttttttcca
     2941 gtaccactcc cacgcttccg ggttaattgg ctcgcccacg gaaccgagaa tgcgcagcga
     3001 cgaacggtcg gtaccctcga tcgctttatc gccttccgcc atcagcgcgc ggatcgccgt
     3061 gggtgcggta tagagaatat tgacctgatg cttgtccacc acctgcgcca tacgggcagg
     3121 cgtcggccag ttgggtacgc cttcaaacat cagcgtggtc gcaccgcagg ccagcgggcc
     3181 gtacagcaag taactgtgtc cggtcaccca gcccacatcg gcggtgcacc agtagatatc
     3241 acccggatga taatcaaaga catatttaaa ggtcagcgcc gcgtacacca gataaccgcc
     3301 ggtagtatgc agcacacctt ttggcttacc ggtagaaccg gaggtgtaga gaataaacag
     3361 cggatcttcg gcgttcatct cttccgcctg gtgctgatcg ctcgcttgct caaccaggtc
     3421 gtgccaccac aggtcgcgcc cttcctgcca gtcaattttc ccgccagtac gcttcagtac
     3481 caccacatgc tctacgctgg tgacgttcgg gtttttcagc gcgtcatcaa cgtttttctt
     3541 cagcggaata ctgcgcccgg cacgcacacc ttcgtcggaa gtgatcacca gtcgtgagtt
     3601 ggaatcaata atgcgcccgg caacggcttc cggcgagaag ccgccgaaaa tcaccgaatg
     3661 caccgcgcca atgcgggcgc aggccagcat cgcaaccgcg gcttccggca ccatcggcat
     3721 ataaatcgcc accacatcac cttttttaat gcccagctcg agcagggtat tggcgaagcg
     3781 gcacacgtcg cggtgcagct ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc
     3841 ttcccagatg atggcggtgc gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc
     3901 cgccagattc agcgtgccgt cctcgtacca tttaatggac acattaccgg gggcaaagga
     3961 ggtatttttc accttctggt acggtttgat ccagtcgaga atttttccct gttcgcccca
     4021 gaaggtatca ggtacgttaa tagattgttg atacatcgcc tcgtactgct gagggtttat
     4081 caggcaacgg tctgcgatgt tggcaggaat ggtgtgtttg tgaatttggc tcatgctttt
     4141 gttctccttg taggatgtta ataatatgtg gcataagcgt taaatgtagg ggtattggca
     4201 gttttgttta gtatttgggc gacagatcac gcaaaagtag aattgtgcaa ataaacggca
     4261 gggtaatttt tgaaggtcac caacaaaagt tgattaattc tttgaggaac atgcagttat
     4321 gcatgctgtt gaaaaagagg aagatactga ctaactctaa agtggtattt tacatgcact
     4381 tacaattgat taaagacaac attcacagtg tggttatatg ttacacatag gggcgagcaa
     4441 tgtcatgaca gtgtaggtgc ggttactgtc gtgaaaacaa taaaaaccgc cattgcaaca
     4501 atggcgcaat tcggatgaag cccctatgac aaggataaaa ataaacgcac gccgtatctt
     4561 cagcttattg attccttttt tctttttcac ttctgttcac gctgaacaaa cggcagctcc
     4621 cgcaaaacct gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc atcccgatca
     4681 atatctctcc tggaaagcca cctcggaaca gtcagagcgt gttgacgccc tggcggaaga
     4741 tccacggctg gtgatcctgt gggcggggta tcccttctcg cgcgattaca acaagccgcg
     4801 tggacatgcc tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg cgccgaaaaa
     4861 cgctgaagat ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg atgtggcgcg
     4921 tctgatccag aaagacggcg aagatggcta cttccacggt aagtgggcgc gcggcggccc
     4981 ggaaatcgtc aacaacttag gttgtgccga ctgccataac accgcctcac cagagttcgc
     5041 caaaggcaaa ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga tggaagccat
     5101 tggtaaacct tttgagaaag ccggacgttt cgaccagcaa tcgatggttt gcggtcagtg
     5161 ccatgtggag tattacttcg acggcaaaaa caaagcggtt aaattcccgt gggatgacgg
     5221 catgaaagtc gaaaatatgg agcagtatta cgacaaaatt gccttctctg actggactaa
     5281 ctccctgtcg aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa cctggacagc
     5341 gggcattcac ggtaaaaaca acgtgacctg tatcgactgc catatgccaa aattgcagaa
     5401 cgccgaaggc aaactctaca ccgaccataa aattggtaat ccgtttgata acttcgccca
     5461 gacttgtgcg aactgccata cccaggacaa agctgctttg caaaaagtgg tcgcggaacg
     5521 taagcagtcg attaacgacc tgaaaatcaa ggttgaagat caactggttc acgctcactt
     5581 cgaagcgaaa gcagcgctgg atgcaggcgc gacggaagcc gaaatgaagc cgattcagga
     5641 cgatatccgt catgcccagt ggcgttggga tctggcgatc gcttcccacg gtattcatat
     5701 gcacgcaccg gaagaaggtc tacggatgct cggtacggcg atggataaag cggcggatgc
     5761 acgcaccaaa ctggcgcgcc tgctggcgac caaaggcatc acccatgaaa tccagatccc
     5821 ggatatctcg accaaagaga aagcccagca ggccattggc ctgaacatgg aacaaatcaa
     5881 ggccgagaag caggacttca tcaaaacggt gatcccgcag tgggaagaac aggcacgtaa
     5941 aaatggtctg ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa acacaatgga
     6001 gtgaatatga gcgtattacg ttcgttatta actgccgggg tgctggcgtc gggcttgttg
     6061 tggagcctga acggaattac cgctacccct gcggcgcagg catctgatga tcgctacgaa
     6121 gttacccagc agcgtaaccc ggatgccgcc tgtctggact gtcataaacc agataccgaa
     6181 ggtatgcatg gaaaacatgc ttccgttatc aacccgaata acaaactgcc ggtcacctgc
     6241 accaactgtc acggccagcc atcaccgcaa caccgcgaag gggtgaaaga tgtgatgcgc
     6301 tttaacgagc cgatgtacaa ggttggagag cagaacagcg tctgtatgtc ctgtcatctg
     6361 ccggaacagt tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa agtggcgtgc
     6421 gccagctgcc attccctgca tccacagcaa gatacgatgc agacgttaag tgacaaagga
     6481 cggattaaga tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa ctttaacccg
     6541 gcgtctgttc cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag tttctcaccg
     6601 gcgtcggcgt gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg aaaacgttga
     6661 atatcaatgg ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc ggctgtaccg
     6721 cctgtatgga tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg cgcctgacga
     6781 ttattcgtag cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc ttccgtaagt
     6841 cttgccagca ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt gcgtcttttc
     6901 gtgatgccgc tagcgggatt gtcgatgtta atcctgatct ctgcgtcggt tgtcagtact
     6961 gcatcgccgc ctgtccgtac cgcgtgcgct ttatccatcc ggtcacgaag acggcggaca
     7021 aatgcgattt ctgccgtaaa accaatttgc aggccggtaa gctgcccgcg tgcgttgagg
     7081 cttgcccgac gaaggcgctg acgtttggca atctggacga tcctaacagt gagatttcgc
     7141 aactgctgcg ccagaagcct acttaccgct acaagctggc gctgggaacc aaaccgaagc
     7201 tgtaccgcgt accgtttaaa tacggggagg tgagccaatg acgcagactt ccgcatttca
     7261 ttttgaatcg ctggtgtggg actggccgat tgccatctac ctgtttttga ttggtatttc
     7321 tgccggtctg gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg cgggcggtgc
     7381 agacagtacg ttgctgcgca ccacgctgat tgtcgggccg ggcgcggtga tcctcggtct
     7441 gttgatcctc gtcttccacc tgacaagacc gtggaccttc tggaagctga tgttccacta
     7501 cagttttacc tcggtgatgt cgatgggggt gatgctgttt cagctctaca tggtggtgct
     7561 ggtgctgtgg ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac aacgctggtt
     7621 gccgaagctg gggatcgtgc aaaaggttct gagcctgctg acgcccgttc atcgcggact
     7681 ggaaacattg atgctggtgt tggcggtgtt gttgggggct tataccggct ttctgctgtc
     7741 ggcgctgaaa tcgtatccgt tcctcaataa cccgatcctg ccggtgctgt tcctcttctc
     7801 cggcatctcg tccggtgcgg cggtggcgct gatcgccatg gcgatacgcc aacgcagtaa
     7861 cccgcattcc acggaagcgc agtttgtaca ccgtatggaa atccccgtgg tatggggtga
     7921 aatcttcctg ctggtggcat tttttgtcgg tctggcgctg ggcgatgacg gtaaagtgcg
     7981 tgcgctggtg gcggcattag gtggcggttt ctggacgtgg tggttctggc ttggtgtcgc
     8041 cgggctgggg ctgattgtgc caatgttgct caaaccgtgg gtcaatcgca gttccggcat
     8101 tcctgccgtg ctggcggcgt gtggggccag tctggtcggc gtgttgatgc tgcgcttttt
     8161 cattctctac gccggacagt tgacggtggc gtaagccaga aaagaggtgg tttctggacg
     8221 tattccttcc tgaagtcggt tttctggcgt tgttgttaag tctcggggtc aacgtgttga
     8281 ccccgttgac ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg cgactcactt
     8341 gcatcggcat tctggcgcag ttcgcgctcc tgctgctcgc ctttggcgta ctgacgtatt
     8401 gttttctcat cagcgatttc tcggtcattt atgtcgcgca acatagctat agcctgctgt
     8461 cgtgggaact caagctggca gcggtgtggg gcggccatga aggttcgctg ctgctttggg
     8521 tgctgctgct ttccgcctgg agcgcgctgt ttgcctggca ttatcggcag caaaccgatc
     8581 cgctatttcc gctgacgtta gccgttttat ctctcatgct cgccgcactg ctactgtttg
     8641 tggtgctgtg gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa ggccgcgatc
     8701 tcaatccgat gctgcaacat cccggtctta tctttcatcc accgctgctt taccttggct
     8761 atggcggttt gatggtagcg gcgagcgtgg cgctggcgag tttactgcgc ggcgagtttg
     8821 atggtgcctg cgccagaatt tgttggcgct gggcactacc tggctggagc gcattaacgg
     8881 cggggatcat cctcggttcc tggtgggcct attgcgaact gggctggggc ggctggtggt
     8941 tctgggatcc ggtggaaaac gcctctttat taccctggct ttctgccact gcgctgctgc
     9001 acagtttgtc cctgacacgc cagcgggaga ttttccgcca ctggtcgctg ttgctggcga
     9061 tagtaactct gatgctgtcg ctactgggta ccttaattgt ccgttctggc attctggttt
     9121 cggttcatgc gttcgcactg gataacgtcc gcgccgtgcc gttgttcagc ctgtttgcac
     9181 tgattagcct tgcgtctctg gctctgtatg gctggcgagc gcgggacggt ggtgcggtgg
     9241 tgcgtttttc ggggttatcg cgggaaatgt taatcctcgc tacgctgttg ctgttttgcg
     9301 cagtgctact gatcgtgctg gtgggaacgc tttatccgat gatttacggc ctgctgggct
     9361 ggggacgcct ctccgttggc gcgccgtatt ttaaccgcgc gacgttaccg tttggcctgt
     9421 tgatgctggt ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgtg cagcttccgg
     9481 cgctggtagc tcatgcgggc gtgctgttat ttgccgcggg gatcgtggtt tccagcgtca
     9541 gccgtcagga gatcagcctg aatttacagc cgggtcagca ggtgacgctg gcaggataca
     9601 ccttccgttt tgagcgcctc gatctgcaag ccaaaggcaa ttacaccagc gaaaaagcga
     9661 tagtggcact gtttgaccat cagcaacgca ttggtgaact gatgccggag cggcgttttt
     9721 acgaagcacg tcgtcagcaa atgatggaac cgtcaattcg ctggaacggc atccatgact
     9781 ggtatgcggt catgggtgaa aaaaccggag cggatcgtta cgcttttcgc ttgtatgtac
     9841 aaagcggtgt gcgctggatc tgggggggag gattgttgat gattgcgggc gcattgttaa
     9901 gcggatggcg ggggaggaag cgcgatgaat aaaggacttc tcacgctact gctgttattt
     9961 acctgttttg cgcgcgctca ggtcgtagac acctggcaat tcgccaatcc gcaacaacag
    10021 caacaggcgt taaatattgc cagccagtta cgttgtccgc agtgccagaa tcaaaactta
    10081 ctggaatcca acgcgccggt ggcagtcagt atgcgccatc aggtttacag catggtggcg
    10141 gaggggaaga acgaagtcga aatcattggc tggatgaccg aacgctacgg agattttgtt
    10201 cgctataacc caccgttaac gggtcagacg ctggtgttgt gggcgctgcc agtggtgttg
    10261 ttactgctga tggcactgat cctctggcga gtgagggcga agcgatgaaa gagcccaaaa
    10321 taccggtgaa aatgctgaca acactcacga ttttgatggt atttctctgt gtcggcagtt
    10381 atctgttaag tccaaaatgg caggcggtac atgcggaata tcagcgtcag cgcgatccgc
    10441 tacatcagtt tgccagccag caaaccccgg aagcgcagct tcaggcattg caggataaaa
    10501 tccgcgctaa tccacaaaac agcgaacaat gggcgttact gggcgagtac tatctgtggc
    10561 aaaacgatta cagcaattcg ctgctggcgt accgtcaggc gttgcaacta cgtggtgaga
    10621 acgctgaact gtatgcggcg ctggcgacgg tgctttatta ccaggccagc cagcatatga
    10681 ccgcccagac tcgcgcaatg atcgacaaag ccctcgcgct ggactctaac gaaatcaccg
    10741 ctctgatgct gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa gccatcgaac
    10801 tatggcaaaa agtgatggat ctcaactcac cccggatcaa ccggacacag ctggtcgagt
    10861 cgattaatat ggcgaaattg ttgcagcgga gatcagatta atcatctggt gatgaagatt
    10921 atgttattga gttgtataaa aaataatcga atgaacatat gccaaaaata atcaccaatc
    10981 agtattattg cagattaaac aaataaaaat ctttccataa caaatggtta ttcattaatc
    11041 ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt
    11101 gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg
    11161 caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg
    11221 ctccattgag gaagttattc tgatgaaaag tataaaattc agcctggcct ggcagattct
    11281 gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg
    11341 cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa
    11401 aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga
    11461 tgccaaacag ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac
    11521 cgtcgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga
    11581 tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca
    11641 aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc
    11701 gtcgatggcg aaaggcgaaa tgctgccgat catcttcttc tcggtgctgt ttggtctggg
    11761 gctttcctcc ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc
    11821 tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc
    11881 gctgattgcg gtgacggtgg ctaactttgg tttctcttct ctgtggccac tggcgaaact
    11941 ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc
    12001 gcgcctgtgc gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct
    12061 ggcgtactcc actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc
    12121 ctacggagca ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct
    12181 tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat
    12241 tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg
    12301 gattgctggc gtgccgggcg tgtcgtttgt ggtgctgctg gcaacgctgg gtagcgtagg
    12361 tattccgctg gaaggcctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg
    12421 tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca
    12481 caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa
    12541 aactgcggat caataattga agattgccgg ggttatccac ccggcaatgt gtggatgcct
    12601 gatgcgacgc tggtgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc
    12661 ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg
    12721 tttgttcttc tcgataaaac cttcctcacc gtttaagaac atcattcccg cccagtactc
    12781 ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc
    12841 gtcggcctta atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac
    12901 ggcggagtca ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata
    12961 atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt
    13021 actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac
    13081 cggattggtg attttgatct gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc
    13141 ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc
    13201 aagaaaatat tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg
    13261 ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt
    13321 cattttgata atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa
    13381 aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag
    13441 gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcggc
    13501 gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa
    13561 gttttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc
    13621 ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg
    13681 cacccaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg
    13741 tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg
    13801 gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag
    13861 tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgt gccagaccgt tcggggtatc
    13921 aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg
    13981 ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca
    14041 cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat
    14101 acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac
    14161 tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc
    14221 ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg gttttggtca taaagatgtc
    14281 ctgaacgata accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcgtc
    14341 agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg
    14401 gtgcggcagc tcgctgatgc gataaccggt atgagcaggc aggctttcca cgccccaggc
    14461 tttggcgaat ttctcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc
    14521 cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac
    14581 acccgcatgc ggcttaccga ggttaccggt cagcatcgcg aggctggtca gagaacgcac
    14641 ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt
    14701 cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacgc cggtgatatc
    14761 ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt
    14821 acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc
    14881 attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc
    14941 ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat
    15001 tacgtgattc gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa
    15061 atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg
    15121 cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc
    15181 aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga
    15241 ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc
    15301 ggcaacgtaa ttcagtgcct catcccagga aacaggttcg agtttgccgc cacgctggcg
    15361 acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca
    15421 gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc
    15481 ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg
    15541 gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg
    15601 ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt
    15661 acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac
    15721 gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg
    15781 ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg
    15841 cggctgtcca gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg
    15901 gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag
    15961 cgcgttgctt ccacgcgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc
    16021 gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc
    16081 atcatgttgc tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac
    16141 agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta
    16201 tccaggtgga tggcatcaag gccgaaaaac gctttgatat caaagctcgg gtagaacagc
    16261 gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga
    16321 tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt
    16381 ggtaatgcca ccggtttgat ctccggcata tcgctggccc ccgcgccaat caatgcacgc
    16441 agagattctc gcgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc
    16501 agaatctgtg cccgcgcgcc ctggaaaggc acttgtgctt ccagaccgtg cgccacttta
    16561 ctctggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc
    16621 atctgatagc tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc
    16681 tctactgctg cggtttctgc cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct
    16741 gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaagctac tgtggcttcc
    16801 gtatagtacg gcccgtccat acccagtgcg ggcgcatcca tcgcataagg gcttaaaaag
    16861 ccgttcgccg agacgcgttg gcggttgagc atccctaacg ctgcgacctg taattgtgaa
    16921 ccggcatcta acaaatcggc ctgggactgc gctttttctt cccgcagttt cgcttcggcg
    16981 agggtgtgtg aaccacttag cgtccgttgg agcagcgcat ccagctgcgg gtcattgagt
    17041 tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc gtcggccagt
    17101 ttgatttgtt ccggtttgag ctgttgatga ggtgcagaat ccttacgtac cagggcacag
    17161 ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga
    17221 ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc
    17281 agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca
    17341 ggatggcctg cgaggcgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg
    17401 ttgccagacc ggcagcgtat ttctccaggg cgtcggcaaa ctgagctgct ctgtccggct
    17461 gtagtgcgtt atccagcgcc tgcgccgagt tccaggtggc atcccaggcg tgaagaatat
    17521 cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt
    17581 ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat
    17641 gcagaccgat acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga
    17701 tccgcattac tttacttaac atacccagcg tgccagccag tttttgcggc agtgtgcgcg
    17761 cttcactttc aggccagaca aaggtgtaaa tcaccgcgga caccacggta ccaatgatga
    17821 tacccagggc gcgatcgcga atttccacca ggtcgtacac tgggccaaaa acgttttcga
    17881 gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc
    17941 tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa
    18001 tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa
    18061 aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt gggttagcga
    18121 cgatcacgca tgtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtaaaagg
    18181 tgtaacagat caaacaggcg agcagcgttt ttaccgcgta gcgcatatag tctggattgg
    18241 taaaagcatc ggcggccatt gatggcggtt tggctgcggg cgttggcggc gtattcgggt
    18301 ccatctgacc cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg
    18361 ccatcgcttc actttcactg atccgccagt cgctttgcca gcactgacct tcagcaacgg
    18421 catgctgcag cttattgatt tctgaggcta atttttgccg aaattcaata attgcctgag
    18481 aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg
    18541 ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa
    18601 agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg
    18661 cgaggctgtc cgtcaggtgg ctaatggcat catcaagccg atcattaagc gcctgatgca
    18721 tttgcgaaat ggcacgactg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg
    18781 gataaaggcc aacaacgata caccacagcg ttaagcgcac gaccacttcc ggatagtcga
    18841 gcatggcggg gaaggtttgc ccgtaaatgg cgacaatggc gactgcgaaa aagaccagtc
    18901 ccagccgatg ggtgcgcatc aaaaacatac agcccatcag gatcggcccg gcgatgaaca
    18961 atcggatcaa cggttcgccg tatgaccatt tgtagatcaa aaacaggctg ccgatctcca
    19021 gcatcgtggc gaccacaaat aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa
    19081 taccgtaaaa cagcactgcc agcgataacg ccacgaaagg gatctcaaag gtcatcgaga
    19141 tcagaatcac cagcaggcag cctacccaga gttgcacggt ctgcggcacg cggcctggtc
    19201 gccgctcgct taactcttca tgaaagaacg ccagcagcct gaccaccggt aatggcaggg
    19261 agttgagcgc gctcattatt gcggctcaag attagcgact gccgaagcgc cgatgcggaa
    19321 catttccggg tcaggtttat cgaccatgat tttgaccgga aaacgctggg caacgcggac
    19381 ccagttaata gagcgagaaa ctttcggcag gccgcccagc accaggccgc cgtcatccgg
    19441 tagcacgccg tagccaatcg aatccacttt accctcgaag gttttgccgc tgtcactcat
    19501 caggcgaatc gttgcgggtg tacctgagcg aatatttttc agatcggttt cgcggaagtt
    19561 ggcgatcacg taccagtgac gagtgtcgat tagggtaaaa atagggcgca tggcagaagc
    19621 aaattgcccg acggaggttt tgagggaaat gacccggcca tcaaacggcg cgcgaacggt
    19681 cgccatttcc agatgcagtt tggtcagggc aatatccgct tcgaccgccg cacgctgggc
    19741 aactaatgca tccacgccgc tgacggcgct ggcggctgac tgcgcctgca acaatacagc
    19801 attaagatcg gcttctgcag cgcgctgcgc ggtcctcgca cggtcaacgt cttccgctga
    19861 gacaaaacct tctttcagta atggctcggt gcggcgtaat gtatctgtgg cctgtttcgc
    19921 ggcggcacgg gctttttcta ccgtggcgtt aaccgagtcg gcaccaaact gttgcgcgtc
    19981 aacgctacgc tgggtgagca taatttgctt atccagcgcc gcgagggagg cttcagcttt
    20041 cgccagattg gcttcgtacg ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac
    20101 tgcctggttg tcggtgaccg ccagttctac aatgcgaccg ctgacttccg gcaccacatc
    20161 aatggtatct gctgacgcgt aagcgtcatt agttgatggc gcactgtcta cgcgccagat
    20221 aacgaaaaca agggcaacca gcgccaacgc aaccactaac agagcaggga atttactgcg
    20281 aggagctttt ttcggcgtac tttccatgag cgcgtctcaa atagattaga aaaatgccag
    20341 ccagaacagc atggcgtaga gagcaaaaag ggcggtataa ataattccga caaatgccag
    20401 attgatattt gcccgctgaa taactcgcct cgtgatgagc atcaaaataa ggctggcaat
    20461 ggcgcagaaa aaccagctgg gataataagc accgatcacc gggattgccg gagataatga
    20521 gcatcccgaa accatgaaga cgggaattat ccaggctgtt tttttgagtt gcatcgccat
    20581 gcgagagaga actgttggca tattaaatac atcctgtct
//
LOCUS       DAZFJF010000079        20602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFJF010000079 DAZFJF010000000
VERSION     DAZFJF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.079"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..757)
                     /gene="aroD"
                     /locus_tag="ACHKAU_003199"
     CDS             complement(<1..757)
                     /gene="aroD"
                     /locus_tag="ACHKAU_003199"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO5532208.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(788..1654)
                     /gene="ydiB"
                     /locus_tag="ACHKAU_003200"
     CDS             complement(788..1654)
                     /gene="ydiB"
                     /locus_tag="ACHKAU_003200"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO5532209.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1666..2931)
                     /locus_tag="ACHKAU_003201"
     CDS             complement(1666..2931)
                     /locus_tag="ACHKAU_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5532210.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3158..4372)
                     /locus_tag="ACHKAU_003202"
     CDS             complement(3158..4372)
                     /locus_tag="ACHKAU_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5532211.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4472..4828)
                     /locus_tag="ACHKAU_003203"
     CDS             complement(4472..4828)
                     /locus_tag="ACHKAU_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO5532212.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4964..5071)
                     /gene="rprA"
                     /locus_tag="ACHKAU_003204"
     ncRNA           complement(4964..5071)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKAU_003204"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5257..6369)
                     /gene="ydiK"
                     /locus_tag="ACHKAU_003205"
     CDS             complement(5257..6369)
                     /gene="ydiK"
                     /locus_tag="ACHKAU_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO5532213.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6758..9814
                     /locus_tag="ACHKAU_003206"
     CDS             6758..9814
                     /locus_tag="ACHKAU_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO5532214.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9811..10221
                     /gene="menI"
                     /locus_tag="ACHKAU_003207"
     CDS             9811..10221
                     /gene="menI"
                     /locus_tag="ACHKAU_003207"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO5532215.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10321..10509
                     /locus_tag="ACHKAU_003208"
     CDS             10321..10509
                     /locus_tag="ACHKAU_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO5532216.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11057..11425
                     /gene="sufA"
                     /locus_tag="ACHKAU_003209"
     CDS             11057..11425
                     /gene="sufA"
                     /locus_tag="ACHKAU_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO5532217.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11434..12921
                     /gene="sufB"
                     /locus_tag="ACHKAU_003210"
     CDS             11434..12921
                     /gene="sufB"
                     /locus_tag="ACHKAU_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO5532218.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12931..13677
                     /gene="sufC"
                     /locus_tag="ACHKAU_003211"
     CDS             12931..13677
                     /gene="sufC"
                     /locus_tag="ACHKAU_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO5532219.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13652..14923
                     /gene="sufD"
                     /locus_tag="ACHKAU_003212"
     CDS             13652..14923
                     /gene="sufD"
                     /locus_tag="ACHKAU_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO5532220.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14920..16140
                     /gene="sufS"
                     /locus_tag="ACHKAU_003213"
     CDS             14920..16140
                     /gene="sufS"
                     /locus_tag="ACHKAU_003213"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO5532221.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16153..16569
                     /gene="sufE"
                     /locus_tag="ACHKAU_003214"
     CDS             16153..16569
                     /gene="sufE"
                     /locus_tag="ACHKAU_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO5532222.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16718..17722
                     /gene="ldtE"
                     /locus_tag="ACHKAU_003215"
     CDS             16718..17722
                     /gene="ldtE"
                     /locus_tag="ACHKAU_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO5532223.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17786..18022)
                     /gene="lpp"
                     /locus_tag="ACHKAU_003216"
     CDS             complement(17786..18022)
                     /gene="lpp"
                     /locus_tag="ACHKAU_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO5532224.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18333..19745)
                     /gene="pykF"
                     /locus_tag="ACHKAU_003217"
     CDS             complement(18333..19745)
                     /gene="pykF"
                     /locus_tag="ACHKAU_003217"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO5532225.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19753..19947)
                     /locus_tag="ACHKAU_003218"
     CDS             complement(19753..19947)
                     /locus_tag="ACHKAU_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532226.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            20302..20511
                     /gene="fumD"
                     /locus_tag="ACHKAU_003219"
     CDS             20302..20511
                     /gene="fumD"
                     /locus_tag="ACHKAU_003219"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO5532227.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5405 a         4921 c         5266 g         5010 t
ORIGIN      
        1 atgcctggtg taaaatagtt aataccgtgc gtaaatcatt taccgagatt tgccctggtg
       61 cagaggcttt ttttaccgca ccaaaagttg ccgccgagcc aaatacttca ccagccagac
      121 gagaaattac gccagttttt gccatcgaca tcgtgatgat tggacgatcg gcatactgct
      181 cctgcatctc cagggtcgcg gtaagcaacg tcagcacatc gctggtactt tgcggcatca
      241 gcgcaatctt aggaatatcg gcgtcgaagg actgcatttt gcgcagacgg gcaatgattt
      301 cttcggcttc cggcgtttta tggaagtcat ggttggacat gacaactttc acatcatgcg
      361 cgtgggcgta ggcgacggtt tctttaacct gatcatcgcc ggtaaataac tccagatcga
      421 tcatatcaac caggccgctg tcgatggctg cacgattgag agcaatataa gcctcggtgg
      481 aaatcgcctg ctcgccgcct tctttggcac tgcggaaggt aaacagcagc ggtttttctg
      541 gcatggtttc acggagaatt tttgccgccg ccatgacaga ctccacattg gagaggtcgg
      601 caaagtggtc cacacgccat tccagaatat caaagtccgc ttcacgatag gcgagagctt
      661 cggatttcac gcgggcgata tctttcgcca tcagcgagac gatgatttta ggtgcgcccg
      721 taccaatgac gagatctttt acagttacgg ttttcatttt ttaccctttc tgcacgcggt
      781 cagcctgtca ggcaccgaac cccatgacct gtttaacata ttccagaggg aaatctttgc
      841 cagtccataa cgtgaactgt tcagcccctt gccacaacaa catgccgtat ccatcaatcg
      901 ttttgcaacc agcttgttgc gcctgctgca ataacttcgt catatgcggg ttatacacgc
      961 attcagtgac cagcagtccc ggatgtaaca gactgatatc attaactaat gattcattct
     1021 caaggggttt catacccact tttgtgccat tggttaaaat gtcggcggaa gccagggctt
     1081 cagcaaaggc ttgctgatcg gcgagatcgg taaccgtgac gacacaatcg gtgttttcgt
     1141 taacccgctg cgcgaaggcg agggctttat cgaagaactc atcccgacgg ttaaagagtt
     1201 taatttcttt taaaccttca attgccccct gcgcgccaat cgccgttgag gcacctccgg
     1261 cccctaacag caccattgtt ttgcctttga tatcaaaacc gctctcttta atggcgcgaa
     1321 tatggcccgt gccgtcggtg ttatagccgc gcaggtagcc atcatcatta acgatggtgt
     1381 tgatggcacc caccagtttg gctgctggcg ttaattcatc aacatattca cacgccagtt
     1441 gtttgttcgg cattgatata ccagttccgc gcattttgag ggcttttaat ccttcaattg
     1501 ctccgggaaa actatcgtta tccacttcaa aggccatata ggtaaatggc aatcccgctt
     1561 tttctaaggc tttattctgc atttcaggcg ataaactgtg gcggatagga taggccatca
     1621 gcccaatcaa ttcgtatttt gcggtaacat ccataattaa ctcctttaac ctctatgctt
     1681 aattgtgttt aaccgggtgc taaatttacg ctcgccaaac cgcacatcat tctcaggaat
     1741 aataaaaacg cggtaatagc ggataaaaac aataattgcg gtaatcaggg ccagcagcgc
     1801 gaaagtaaaa tcgagaacaa tgatatattg aaggccgatg ttcgacagat aaccggtaat
     1861 cagcggaata acaaagttag ccagtccacc catcatcata taaatactgg tgactttggc
     1921 tttgcttttt gggaaaaact ctgacatgac cgaaacaccg agctgtaaaa tgccgccagc
     1981 tgctgagaaa ccgataacaa aggcgccggc attacacacc agtggagaag ggtacaggta
     2041 gataatggct gctgttattg ttgccagtgc agagttaaat acattagccc agatgggccg
     2101 gaccattttt ttcagtagtg cggcaaaaat aaagacacag accaacgagc ccatactgta
     2161 ataagagatg gtttttaatg cctcagcttc tgacatacca gcaaaagcca tcgcatattt
     2221 gggcatccac accacaatca cataaaaggt cgagaatgcg gctacaccga acagtaccga
     2281 tgaaacacct tccagccaga ctaacggttt gctgttcatt tgcggtaatt cattagctac
     2341 gctggcgtcc accaactggc tggggaattt gcttttcaac agcatcagcg tgatcagtac
     2401 aaacagaata cccggaataa tcagcccata gccgtaccag atattattga gcaacatata
     2461 gctcaccagc attgggtaga acatttgccc aaatgacacc atcgctttaa ccagtatgac
     2521 cgccgaacca gaggctttcg gaaagcattc catgagcgcg gggtagccac ccgtatccag
     2581 cgctgagtta gcgataccta cgcacactgc cagaccgtag gcgagagtta aattcgggca
     2641 agcgggaata ccaaagaaga atagcagata cattattact gccattaata tcaccgcccg
     2701 acgaccaaac ttatcggaga tcacaccgaa gaataaaata ctgatcaatc gccccaaacc
     2761 aataccggaa attaagtagg caatgcccgc gttgtcagtg gaaaactttt ccgccagaga
     2821 tgacatgttt tgggcaagcg taataacact aatgccgtgc aggaagtagc tgaagtaaat
     2881 acaaagaaca gccaggataa atggcgtgct gaaagcctta ttttgagaca ttttcacagc
     2941 tccttgctga aagataattt cgttaccgta cgtattgttc ttcgacggtt tacataatta
     3001 ctatactgaa gatgattttc cctgggtaca tggcataaag tttatgtttc caggaatatt
     3061 gcatgtcata gtttgttttt gatcgcgatt ttccttagtt agcgcagaaa gagttattta
     3121 tccgtaagat cttgcatgac ctgaaaagaa tattgcatta cccacccgga gcgacatttt
     3181 cctttagcga gtgatgccgc gtttttttgc ggctgcgtaa gccgataaac agtgccagta
     3241 aaaaaccgat ggcagcgatg gcggtatcga accacataat atcggcaata cttctttggg
     3301 acagatgagc cgtaatcaac ggaatagtaa aggtcgcaat actgcccgca ctgtaataga
     3361 tccctgtagc tttaccttta gcgtaaggga aacgttcagc cattaacgtc aggccaattt
     3421 gcacaacacc tccagcagag gtaaaaccaa tgacaaaagc aaatattatc accacataaa
     3481 atgtgggatg caggcagacg gtaaacagag caataaatga gataaaggtg tacagcatca
     3541 gtaatgttgt tgggcgaacg gtattacgaa tgagtggagc ggtaataaat acacaaagca
     3601 gcgaatccac ggtgtagata ctgagtagtt taatcgacat agtgtatgac atgcctgcaa
     3661 caaattgtcc gtactgtgcc agccactggc taaccagata aaacgttgcc attgagatat
     3721 agccatataa ggtataactg gctaaatcga taattgaaca gcgatgttcc gtagagctgg
     3781 tggttttctt tatgacaggt aagcgatgac ccggatgggg tgggaacgta caacggtata
     3841 aaaacagagc gttaataaac ataatgcctg cagcaatcat aaaggaccaa ccgaaccaca
     3901 gttcagccca cactaacagg ctaatgatta gcggtaataa aaattgtccg ctggaaacaa
     3961 atgctttaat taaaatattg gctgtcccag gtgagcgtgg aaaagcttcc atcaaactgg
     4021 gataagtgcc tgcatcgaga aaactgtttg ccattcctgc cagaaagcca aaaacataag
     4081 cgataatgat gttattggtg tgcaggatgc caaaaaagaa ggccatatag cagcacatcc
     4141 cgagcatgat aaaagggcgg cgaccaaagc gatcggataa taatcctgca aaaagcaaga
     4201 cacttaatcg accaatgccc agcgatgaga taactatcga gacacccgcg gcattagtct
     4261 gccaaagtgt ctccagcgag gccatattca ggctcatcaa aatgacgccc ataccatgca
     4321 ctaggtaatt aaaatacaac ccaagtgcgg tagggtaata gggatttttc ataggttcta
     4381 cttagcaaag gtgctttaaa aagtgtagcg atgttgtgtc agtatccgtt cattattgtt
     4441 gtaacaacga caattccttt ttgtggagaa attaacaaag ccgctccaga tcgtgtgcat
     4501 ataattcagc ggctaccgat tgatagatct tccagtcaat aaaattgcca tcagggtaga
     4561 cttgttgaaa cgcggttaag tcggggaaaa aacgcatggt gttgttgccg atgcggttat
     4621 ttattttctc tatgatagca tgaagatgtt tttttcgttg ctgacgcata gccaacagtt
     4681 gctcgactac acgatcagga atggcgcatt tgccattttc ccactgacgc cagctttcac
     4741 tgtcaccggt ctgggcaatc caggtcgcgc attcatcaat agtcatggca aaaatatggc
     4801 gaagtgcctg gagttcataa gcgttcatcg tgtaatggct gcatgaaata aacaataaaa
     4861 tataaataac attatcagcc tgctgacggc ttgaagagag tcacagtatc ttgtgcaaca
     4921 ttattggaaa gagtgagggg cgaggtagcg aagcggaaaa atgttaaaaa aaagcccatc
     4981 gtgggagatg ggcaaagact acacacagca attcgttgtt tcactcaggg gatttccatg
     5041 cttataaatc aatatgttga tttataaccg taagctaata gtaggcatac ggactttttg
     5101 cgtcgatcag attcgtctca ttagtttaaa aactgtaaag aatttgcgac aggtactacc
     5161 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcgggg
     5221 gttaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
     5281 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
     5341 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
     5401 cccgaaagca atcaaaccac caataacacc agagaggatc aggatcagcg gtaaatcggc
     5461 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
     5521 cactaacaat accgttcccc aggtggtatc gccagtccag tagagccaga taatcgccgg
     5581 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
     5641 caacgttgca taaggtacgc cggatacggc gaggccgata ccgccaagca ctgcctgtac
     5701 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagtac
     5761 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
     5821 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
     5881 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
     5941 gcggactttc gccatgatcg ccgtcccccc catatccagc aagttgtgcc agcctgcata
     6001 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
     6061 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
     6121 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     6181 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     6241 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     6301 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     6361 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     6421 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     6481 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     6541 cgatttactc gtcgtatttc aagttgcatg tgctgcggct acgttcgttc accccagtca
     6601 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     6661 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     6721 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     6781 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     6841 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     6901 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     6961 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgtg gcggcggcac
     7021 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     7081 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     7141 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcgccgg aactttcgac
     7201 cagcaaccgg gcaacgctcg gtgggatgat caataccgat gcttctggtc agggatcgct
     7261 ggtctatggc aaaacgtcgg atcacgtact tggcgtacgt gcggtgttgt tggggggcga
     7321 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aatccaatac
     7381 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtcagcaac gccagttaat
     7441 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     7501 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     7561 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     7621 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     7681 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     7741 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     7801 gaacattgtg gagtttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgctct
     7861 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     7921 gtgccgcgag ctggcgggtg ttgaacgtat ctatgcgatg cgtaaaaaag ccgttggtct
     7981 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     8041 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     8101 aagctacggt atgttcggtc acgtcgacgc aggtgtcttg cacgtccgtc cagcgctgga
     8161 tatgtgcgat cctcaacaag agattttgat gaagcaaatc tctgatgacg tagtggcgct
     8221 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     8281 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     8341 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     8401 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     8461 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     8521 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     8581 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     8641 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttgcggacgt taattgcccg
     8701 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     8761 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     8821 tgtgccggag tttcgctctc gttttctgca gctctatcac acccgttatt tacgcccgct
     8881 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcgccg ctgatggcac gcgcgccgaa
     8941 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     9001 catggttgat ttgccgctgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     9061 ctcggcaaac atgacgctgg aacagcttga atctctcaat gcagagcaga aagcgcgcac
     9121 agtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     9181 tgtccgtctg gtcgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     9241 caaagcccag catattaaag gttttcttaa tcgttttgcg aagacggcga aaaagacggc
     9301 ggatttcctc aaccgtatgg cgaagctggg tatgccaatg gtgggcgtcg atccggcgct
     9361 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     9421 cgtcttactg gcgaatgaat ggctggcaag cgcacttgag tcacagccag tggctacagt
     9481 cagcggtgaa tcatggtatt tctttggtca ctgtaccgaa gttaccgcct tgccgggtgc
     9541 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     9601 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccata aaaattcgct
     9661 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     9721 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     9781 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     9841 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     9901 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     9961 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
    10021 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taaccacgtc
    10081 cgctcggcac gagaagggcg ggtgcgcggg gtatgcaaac cgttgcatct cggttcgcgt
    10141 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
    10201 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcctttggt
    10261 cattatctgc acagtaaacg gtggtatact gtgcaggtct tgtctaaaag cgaggatgct
    10321 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
    10381 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
    10441 ctgcttactc tctcttctgc tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
    10501 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
    10561 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
    10621 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
    10681 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
    10741 gcctgtgttg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
    10801 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
    10861 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
    10921 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
    10981 gtttgctggg ttgtactgat aatcattatc actaacatgc tgttatacgc tgaaagcgat
    11041 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
    11101 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
    11161 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
    11221 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
    11281 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
    11341 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
    11401 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
    11461 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
    11521 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
    11581 cgtaatgagc cggagtggat gctgaagttt cgtctaaacg cctatcgcgc atggctggag
    11641 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagc
    11701 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
    11761 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
    11821 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactcagtt
    11881 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
    11941 ggtgaggcga tccacgatca cccggaactg gtgcgtaaat atctcggcac cgtggtgccg
    12001 gggaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
    12061 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
    12121 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
    12181 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcagtggtg
    12241 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
    12301 ggcgataaca acaccggcgg tattctcaac ttcgtcacca agcgtgcttt gtgcgaaggc
    12361 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt ccgcgattac gtggaaatat
    12421 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
    12481 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
    12541 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
    12601 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
    12661 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
    12721 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
    12781 caacgcggga tcagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
    12841 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
    12901 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
    12961 gtggaagata aagctatcct gcgtggatta agcctcgccg ttcgtcccgg cgaagttcac
    13021 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcgc
    13081 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcactg
    13141 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
    13201 ccaggcgtca gtaaccagtt tttcctgcaa acggcactta atgcagtgcg cagctatcgc
    13261 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
    13321 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
    13381 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
    13441 gagtcggact ccgggctgga tattgacgca ttaaaagtgg tcgccgatgg cgtgaactcg
    13501 ctgcgtgatg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
    13561 atcaagcctg attacgtcca tgtgctatat cggggacgaa ttgtgaaatc cggcgatttc
    13621 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaacgc
    13681 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
    13741 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
    13801 atatacgccg ctggaagggc tgaccaatag ccagtttgtc agcattgcgg gagagatatc
    13861 cccacagcag cgagatgcct tagcgttaac gttagacgct gtgcggctgg tatttgtcga
    13921 tggacgttac gtgtcagcac tgagcgatgc gactgaaggc agcgggtatg aagtgagcat
    13981 taacgacgac cgtcaggggg tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
    14041 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
    14101 gccattgctg ttaatgcata ttacccaggg cgtggcaggt gaagaggtca acactgccca
    14161 ttaccgacat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
    14221 cagccttaat gatgctcgtc acttcaccgg cgcacggttc actatcaatg tcgcagcgaa
    14281 cgcccacttg cagcatatca agctggcgtt tgaaaacccg gtcagtcacc actttgccca
    14341 taacgatttg ttgctggctg acgatgccac cgcatttagc catagtttcc tgctgggtgg
    14401 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
    14461 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
    14521 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
    14581 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
    14641 gaccaacaat aatctgctga tgggcaaact ggcagaagtg gatacgaaac cgcagctgga
    14701 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
    14761 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
    14821 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
    14881 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
    14941 gtgcgggccg actttccggt gctttctcgt gaggtaaacg gtttgccgct ggcttatctc
    15001 gacagcgccg ccagtgcgca gaaaccgagc caggtgattg acgccgaggc cgagttttat
    15061 cgtcatggct acgcggcggt gcatcgcggt attcatacct taagcgccca agcgaccgag
    15121 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    15181 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ctggggtaac
    15241 agcaatgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    15301 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat cccgctcaat
    15361 cccgacggta cgttgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    15421 ctggcaatta ctcatgtctc caacgtgctt ggcacagaaa atccactggc ggaaatgatc
    15481 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    15541 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    15601 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    15661 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cacaacctgg
    15721 accaaagcac cttggcggtt tgaagccggt acacccaata ccgggggcat cattggtctt
    15781 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    15841 aatctgatgc actacgcgct atcacagctg gaatctgtac cggatctcac tctctatggt
    15901 ccgcagaaca ggcttggcgt tattgctttt aatcttggta aacaccacgc ctatgatgtt
    15961 ggcagttttc tcgataacta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    16021 ttaatggcct attacaacgt ccctgctatg tgtcgggcgt cgctggccat gtataacacc
    16081 catgaagaag tggatcgtct ggtgaccgga ctgcaacgta ttcaccgtct gctgggataa
    16141 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    16201 tgcgccaatt gggaagagaa atatctctac attattgagc tgggccagcg tctgccagaa
    16261 ttacgcgacg aagacagaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    16321 attgtcatgc gccagaatgc gcagggaatt attgaattgc agggcgacag cgatgcggcg
    16381 attgtgaaag ggcttattgc ggtcgtcttt attctctacg atcagatgac gccgcaggat
    16441 attgtcaatt tcgatgtgcg tccgtggttt gaaaaaatgg cgctcaccca acatctcacc
    16501 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    16561 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    16621 tttacactga cctgcacagg agtgtttcgg tttcaggtga acataagcgc catccgacgt
    16681 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    16741 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    16801 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aggaagggga
    16861 taaaaacctt caggccatcg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    16921 caataacact atcgccccgg tgccaaaacc cggtacgacg ataactattc cttcgcaact
    16981 gttattacct gatgcgccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    17041 ttattatccg ccgggagaaa atatcgtgca ggtttatccg ataggtattg gcttgcaggg
    17101 gctggaaacg ccggtgatgg aaacgcgtgt ggggcagaaa atccctaacc caacctggac
    17161 gcctactgca ggcattcgtc aacgttcgct ggagcgtggt attaaattac cgccagtcgt
    17221 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    17281 cgaatacctc attcatggca ccagtgcgcc ggacagcgtc ggtttgcgcg tcagttcagg
    17341 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    17401 gccggtgaaa gtgatcaacg aacctgtgaa atattccgtg gagcctaacg ggatgcgtta
    17461 tgttgaagta catcgaccac tatcggcaga agaacagcag aacgttcaga caatgccata
    17521 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    17581 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    17641 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    17701 tatgttacgc gcgacgcagt agcggtaaac ggcagacaaa aaaaatggcg cacaatgtgc
    17761 gccatttttc acttcacagg tactattact tgcggtattt agtagccatg ttgtccagac
    17821 gctggttagc acgagctgcg tcatctttag cagcctgaac gtcggaacgc attgcgttca
    17881 cgtcgttgct cagctggtca actttagcgt tcagagtctg aacgtcagaa gacagctgat
    17941 cgattttagc gttgctggag caacctgcca gcagagtaga acccaggatt accgcgccca
    18001 gtaccagttt agtagctttc attattaata ccctctagat tgagttaatc tccatgtagc
    18061 gttacaagta ttacacaaag ttttttatgt tgagaatatt tttttgatgg gaaggcactt
    18121 atttttgatc gttcgctcaa agaagcatcg aacgctgggt tttagataaa aaaattgaga
    18181 gaaaacagga tgcttccatc ggattcatct tagataaagt gagattatat agtgaaatcc
    18241 gatttgattt tttattgctt ctggttatcg attaaataaa aaaagcgccc atcagggcgc
    18301 ttcgatatac aaattaattc acaaaagcaa tattacagga cgtgaacaga tgcggtgtta
    18361 gtagtgccgc tcggtaccag tgcaccagaa accataacta caacgtcacc tttgtgtgcc
    18421 agaccgctct gcagagccag ttctttaccc agacggtaga aatcatcagt agaagtgatc
    18481 tctttaacca gctgcggcac aacgcctttg ctcagtacca actgatgagc cgttttttcg
    18541 ttggtggtca gtgccaggat ggtggcatcc gggaagtatt tacgtactgc gcgagcagat
    18601 ttaccgccct gagtagcaac cacgatcagc ggagcatcca gtttttcagc agtttcaacg
    18661 gcaccacggc ataccgcttc ggtaatgcgc agtttacggt tgtcattgtt gaactcgaga
    18721 cggctgttca tcacgcggtc ggtacgttcg caaatggtcg ccatgataga aaccgcttcc
    18781 agcgggtatt taccttttgc ggattcacca gacagcatca ctgcatcagt accgtcgagg
    18841 atggcgtttg caacgtcacc ggcttctgcg cgagtcgggc gtgggttttt gatcatggaa
    18901 tccagcatct gggtcgcagt gataacgact ttacgtgcac ggatacattt ttcgatcatc
    18961 atcttctggg cgaagataac ttcttctacc gggatttcta cacccaggtc gccacgcgca
    19021 accatgatgc cgtcagaggc ttcgaggatt tcgtcgaagt tgttgaggcc ttcctggttt
    19081 tcgattttgg agatgatgtg gatgttttcg ccgccgtgcg ctttcaggtg ctcacggatt
    19141 tcgataacgt cagaacgctt acgaataaag gaagcagcaa caaagtctac gccttgttcg
    19201 caaccaaaga tcaggtcctg tttgtctttt tcagccagtg ctggcagagc aatggaaacg
    19261 ccaggcaggt tcacaccttt gttttcgccc aggtcaccgt tgttcagcac tttacagata
    19321 actttgttac cttcaatggc ggtaacttcc ataccgatca gaccatcgtc aaccagtacg
    19381 gtgttgccaa ctgacaggtc agtagtgaaa ccttcatacg ttaccgcaac catttcgctg
    19441 ttgccgataa cagatttatc agtggtgaaa gtaaaggtct gaccagcttt cagagaaacg
    19501 tcgttaccgc cttccagttt catggtgcgg atttccggac ctttggtatc aagcaggata
    19561 gcggcggttt taccagtttt gctcatcacg ttgcgcagat tctgaatgcg ctgaccgtgt
    19621 tctgcatagt caccatgaga gaagttcaga cgcataacgt tcatgccagc gtccagcatt
    19681 ttagctaaca tctcttcaga ttcggttttc ggtccgatgg tgcaaacaat tttggtcttt
    19741 ttcatgacag tcttagtctt taagttgaga aggatgggag aaacttgctt tctgggcgca
    19801 ctgccggaaa gcgaatccgg tattacgaaa gtggtggtgc ctcgctctaa ggataggtga
    19861 catcgaaaaa gcgtgcagag gaatgtgtgc ttgtgtctca gcccaacgtg agtgcctgtt
    19921 tttgagtcgt tgagttctac agtccaaagt gctgaaacca ttcaagagtc aattggcgcg
    19981 cattatacgc tgaaattaat ccaaaaggaa aacaaaaaca gcgtttcaaa aaatatacaa
    20041 cttgttagac aataaattgt tatcaaagat ttaacgttcc agggaaaagg ttacgcaaaa
    20101 acttcgctgt ttgcgcaacg tctggtgcca aagttgtcag tgtgaggata agtgaaagtt
    20161 catccggtgt ataaatatat catcgctcaa ttgctatagt taaaataatt tatcgcgtta
    20221 tttgtctgat tttgtttgat ttttgtttaa ttggttagat tgatatatgc tgatagttgg
    20281 catgataaat aggtataaag aatgggaaat cggacaaaag aggatgaact gtaccgagaa
    20341 atgtgcagag ttgtcggtaa agttgtgctg gaaatgcgcg atctggggca ggaaccaaag
    20401 catattgtta tcgcaggcgt attacgtaca gcattagcca ataaacgcat tcagcgcagt
    20461 gagctggaaa agcaggcgat ggaaaccgtc attaacgcac tggtaaaatg accattcagg
    20521 atgaggcggt aagcttcatc ctgatattct tgcttcagtt gcggtccagt taatcattct
    20581 aaataacaac tgctgaaatt at
//
LOCUS       DAZFJF010000080        20472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFJF010000080 DAZFJF010000000
VERSION     DAZFJF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.080"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(402..1598)
                     /gene="hemY"
                     /locus_tag="ACHKAU_003220"
     CDS             complement(402..1598)
                     /gene="hemY"
                     /locus_tag="ACHKAU_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO5532228.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1601..2806)
                     /gene="hemX"
                     /locus_tag="ACHKAU_003221"
     CDS             complement(1601..2806)
                     /gene="hemX"
                     /locus_tag="ACHKAU_003221"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO5532229.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(2828..3568)
                     /gene="hemD"
                     /locus_tag="ACHKAU_003222"
     CDS             complement(2828..3568)
                     /gene="hemD"
                     /locus_tag="ACHKAU_003222"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO5532230.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3565..4506)
                     /gene="hemC"
                     /locus_tag="ACHKAU_003223"
     CDS             complement(3565..4506)
                     /gene="hemC"
                     /locus_tag="ACHKAU_003223"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO5532231.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4893..7439
                     /gene="cyaA"
                     /locus_tag="ACHKAU_003224"
     CDS             4893..7439
                     /gene="cyaA"
                     /locus_tag="ACHKAU_003224"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO5532232.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHIDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            complement(7479..7799)
                     /gene="cyaY"
                     /locus_tag="ACHKAU_003225"
     CDS             complement(7479..7799)
                     /gene="cyaY"
                     /locus_tag="ACHKAU_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO5532233.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7847..9172)
                     /locus_tag="ACHKAU_003226"
     CDS             complement(7847..9172)
                     /locus_tag="ACHKAU_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO5532234.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(9169..10677)
                     /locus_tag="ACHKAU_003227"
     CDS             complement(9169..10677)
                     /locus_tag="ACHKAU_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO5532235.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11015..11569)
                     /locus_tag="ACHKAU_003228"
     CDS             complement(11015..11569)
                     /locus_tag="ACHKAU_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO5532236.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11919..12122
                     /gene="yifL"
                     /locus_tag="ACHKAU_003229"
     CDS             11919..12122
                     /gene="yifL"
                     /locus_tag="ACHKAU_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5532237.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12159..12983
                     /gene="dapF"
                     /locus_tag="ACHKAU_003230"
     CDS             12159..12983
                     /gene="dapF"
                     /locus_tag="ACHKAU_003230"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO5532238.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12980..13687
                     /gene="yigA"
                     /locus_tag="ACHKAU_003231"
     CDS             12980..13687
                     /gene="yigA"
                     /locus_tag="ACHKAU_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO5532239.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13684..14580
                     /gene="xerC"
                     /locus_tag="ACHKAU_003232"
     CDS             13684..14580
                     /gene="xerC"
                     /locus_tag="ACHKAU_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO5532240.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14580..15296
                     /gene="yigB"
                     /locus_tag="ACHKAU_003233"
     CDS             14580..15296
                     /gene="yigB"
                     /locus_tag="ACHKAU_003233"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO5532241.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15380..17542
                     /gene="uvrD"
                     /locus_tag="ACHKAU_003234"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15380..17542
                     /gene="uvrD"
                     /locus_tag="ACHKAU_003234"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO5532242.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17689..18453)
                     /gene="yigE"
                     /locus_tag="ACHKAU_003235"
     CDS             complement(17689..18453)
                     /gene="yigE"
                     /locus_tag="ACHKAU_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO5532243.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18615..18671
                     /gene="ysgD"
                     /locus_tag="ACHKAU_003236"
     CDS             18615..18671
                     /gene="ysgD"
                     /locus_tag="ACHKAU_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO5532244.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18823..19773
                     /gene="corA"
                     /locus_tag="ACHKAU_003237"
     CDS             18823..19773
                     /gene="corA"
                     /locus_tag="ACHKAU_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO5532245.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19815..20307)
                     /locus_tag="ACHKAU_003238"
                     /pseudo
     CDS             complement(19815..20307)
                     /locus_tag="ACHKAU_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
     gene            complement(20304..>20472)
                     /locus_tag="ACHKAU_003239"
     CDS             complement(20304..>20472)
                     /locus_tag="ACHKAU_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO5532246.1"
                     /translation="VAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTN
                     FNTIERIENYL"
BASE COUNT         4812 a         5257 c         5294 g         5109 t
ORIGIN      
        1 cattcataat aagtatgtgt aggatcaagc tcaggttaaa tattcactca ggaagttatt
       61 actcaggaag caaagaggat tacagaatta tctcataaca agtgttaagg gatgttattt
      121 cccgattctc tgtggcataa taaacgggta gatgctcatt ccatctctta tgttcgcctt
      181 agtgcctcat aaactccgga atgacgcaga gccgtttacg gtgcttatcg tccactgaca
      241 gatgtcgctt atgcctcatc agacaccatg gacacaacgt tgagtgaagc acccacttgt
      301 tgtcatacag acctgtttta acgcctgctc cgtaataaga gcaggcgttt ttttatgcat
      361 cagaaaggtc ccggaggtgc ttgcctccgg gtgagaagga actactgtgg tgggttgttc
      421 tgcaacgtta acatcaaacc gtcgcgacgc atcgctgcag cttcttccgg cttgtgcagt
      481 ctgtccagcg cgtcggcaag ccatgcgtaa tcgtaggcgt ccggacgttg tttcagcgcc
      541 gcgcggaagg cgagcgatgc ttcctgccat tctccgtgct tcatcagtga ctggcccagt
      601 gtgctccaca acagcgggcg atcgccgacg tttttgatct gctggcgcag cactttttcc
      661 agctgttccg gattgttggt tttcagtcga ggaatcggca gcagtaggcg atcgtcgtac
      721 tggcgtttca ggccatcgat gataatttgc tgggcagtat catgatcgtc acattcaata
      781 agatgttccg ccattgccac ctgcaacgct acctgatgac gcgttttccg gctttggttt
      841 ttccaccagt tacgcaaacc ttcactaccg ttatcggcac gcgcctgatc catcaggcca
      901 atccatgcct gttgttccag cattgcacga tgttcttcat caccaacatg ggctttcgcc
      961 attgatggga tgatatccag cagcgaactc catgcacctg tgcggatata cgcctgttcc
     1021 gccagacgta atacttccgg atggcgtggc gtaacttcca gcagcttatc cacgccgtgg
     1081 cgtgcagcat ggttttcatt acgggccagt tgcagacgta cacgggtgat ttctaccgga
     1141 atggtgtcat tgccggccag ctctgctgcg cgttccagat gttggttggc gcgtgcttca
     1201 tcaccacgtt gttgcgcggc ttcggcagcc agtagatagt tcaccaccgg ttgttccgcg
     1261 tgatcggcat ttttcgccat cagcttttca acttgctgat aatcgccttc cgccagtttc
     1321 agcagcgcct gttcggtctg cttacgggca cggcgacgct tacgtccgac aaaccaccca
     1381 cgggtgtgtg cgcctgtgcg gaagatccgc cgcagtagcc actcaatggc aaacagcact
     1441 accatcgcca gaatcaaaat aatcgccagg cccgtgacgc tggtttcgat attgtagttg
     1501 tcagtctgga tcagcacgta accctgatga ccggcaatca tcgggccaac cactatcccc
     1561 gcaatcagca aaacaaagag taataacact tttagcatgg ttattctcct tgcggcgcgg
     1621 ctgccggagt atcaccttgc ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtt
     1681 tagcttccgt tgtccccgct gccggttgtg ccagcaggtt acgcacgcgg gtctgcatca
     1741 gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct
     1801 ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt
     1861 aagctcgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctggtgac
     1921 gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat
     1981 cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt
     2041 tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg
     2101 aaagctcttc accgtcggaa tccatcggcg aaccatcgct gtcattatcg gccagacgca
     2161 ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct
     2221 gcgaaactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac
     2281 tcgggtcatt catatccgcc aggctggcat ctgcactttt cagcaacgct gcagcggtcg
     2341 tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctgag
     2401 ccagcagcca ggttttagca tcgctgccgg aaatggtggc gaccttttgt tggacttcat
     2461 ccaactgttt tgccagcgtt tcttgctgac gattcgcctg cttaagctgt gcagcttgtt
     2521 gcttaataat gccttccagc tcggcttttt ggctctcctg ggctttttgc aatgccgtca
     2581 gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc
     2641 catacaaacc gacacccgcc gccagagcaa tagcgatagc caccgcgctg agaatcaatg
     2701 cagtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct
     2761 ccctggtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt
     2821 atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa
     2881 tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc
     2941 gacagtgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca
     3001 acatttcacc gctggtaaca acgaccatcg tcacctcgcg ggattgccag cgcatcgctt
     3061 cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag
     3121 caccgcgcgc tgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca
     3181 gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt
     3241 cccgatcctg cgggtagaga atcttctgtc cgcttacggt atgtagcgcc agtgcggtgg
     3301 tgcgtccaat ggcgaaataa tcaggtagtc ggggccattt acgatcttgc tgatgcagct
     3361 gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca
     3421 gcgccgccag ttgatcagca agttgcggta attgtcgacc cggagaaaac tcaatcagcg
     3481 gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg
     3541 gagacgggcg ggtgaccagg atactcatgc cggggcgtct ccgttataga cttcagcgag
     3601 gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgttcggc
     3661 attttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac
     3721 cagcgcacgc agccagattt caccatcaat aagctcggcg tagctaccaa ttggcacctg
     3781 acatccacct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc
     3841 gtggtgattc agcgcggcaa gcagctcgcg agtgcgcgta tcatcaaggc ggcattcaat
     3901 accgactgca ccttgtccta ccgccggaag agaaatctcg ggtggtaacg cggcgcgaat
     3961 gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc
     4021 gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag
     4081 atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact
     4141 gcctgccggt aacgcatcca gactgtcata attattggac acaaaggcat cgcgaggatc
     4201 ttcacgctca caaatagtga ccaaccccag accttgcggg aattcaaccg gcacatcttt
     4261 cattgaatgt acggcgatat cggcgcgatt ctccaggagc gcgacttcca gctcttttac
     4321 aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt
     4381 caccatcggt accagttcaa ccaccaggcc cggatggctc gccatcaact tgtctttgac
     4441 atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc
     4501 taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga
     4561 tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg
     4621 gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt acagagaata aacggtgcta
     4681 cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt
     4741 tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta
     4801 cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca
     4861 gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac
     4921 agagactgga tgccataaat caattgcgtg tggatcgcgc gcttgctgct atggggcctg
     4981 cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat ccgctaatgc
     5041 cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc
     5101 aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag gacccaccga
     5161 aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa
     5221 gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc
     5281 gccagttact acaacgtaaa tgtagtctgc tggaaagctg ggccgcctcg ctgggtgtgg
     5341 aagtcagctt cttcctgatt gatgaaaacc gcttccgtca taatgaaagc ggcagcctgg
     5401 ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtaccg
     5461 ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc
     5521 attacgacga ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc
     5581 tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc
     5641 agctctacaa gagtatcgat tccccataca aagcggtact gaaaacactg ctgctggaag
     5701 cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc
     5761 acgacggcga gattgtatcg tttggtctcg atccatactg catgatgctg gagcgtgtta
     5821 ctgaatacct gacggcgatt gaagatttta cccgtctgga tttagtacgt cgctgcttct
     5881 atttaaaagt gtgcgaaaag ctcagccgtg aacgcgcctg cgtaggctgg cgtcgcgcag
     5941 tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata
     6001 accgcgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagttg ctcgacgcga
     6061 tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg
     6121 ccagtccgca ggatatcggc gtgctgacgc gtaagctgta tgccgcgttt gaagcattac
     6181 caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga
     6241 cctttattta tgtgccgccg ggccgtgcta accgttcagg ttggtatctg tataaccgcg
     6301 cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaaca
     6361 aactggtggc gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt ttgtatatta
     6421 aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc
     6481 atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagatcc
     6541 gccatctggc gattatcgtt aacctggaat atgacccgac agcggcgttc cgcaatcagg
     6601 tggtgcatat tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc
     6661 tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact
     6721 tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg
     6781 acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg cgcgggttaa
     6841 tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc
     6901 gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg gggttgttct
     6961 tcgaacgcct gaatgtatcg gtacaaaaac tggaaaacgc catcgagttt tatggcgcta
     7021 tttcgcataa caaactgcac ggcttgtcag tgcaggttga aaccaatcac gtcaaattac
     7081 cggcggtggt ggacggcttt gccagcgaag ggatcatcca gttcttcttc gaagaaacgc
     7141 aagacgagaa tggctttaat atctacattc tcgacgaaag caaccgggtt gaggtgtatc
     7201 accactgcga aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc tactcgtcat
     7261 cgcacgatcg cttcacctac ggctcaagct ttatcaactt caacctgccg cagttctatc
     7321 agattgtgaa ggttgatggt cgtgaacagg tgattccgtt ccgcacaaaa tctatcggta
     7381 acatgccgcc cgccaatcag gacaacgata cgccgctatt acagcagtat ttttcgtgat
     7441 gagcgtgccg gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca
     7501 cccgcttgct gtgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg
     7561 caaatccatt catcgccttt taggtcaaaa tggtagccgc cctgtttggt tgccagccac
     7621 acctggtgca gcggctcctg gcggttaata atgattttgc tgccgttttc aaaggtaatg
     7681 gtcagtacgc cgccgttgat ttcgcagtcg atatcgctgt cgccatccca gtcgtccagg
     7741 cgttcttcaa tggtcagcca gagttgatca gccaggcgat gaaattcact gtcgttcatt
     7801 gttgtatcct gtctttaagt gatggcggca gtatagcggt atggggtcag ggcttcaaag
     7861 tttgcccgtc tgttgctgcg ttccggcatg agtcatccgt caacggaata ataatgtctc
     7921 tgttgtgaag ttcacgcaca ttctcttctc tgacgtgtgt tttcgccggg cagggggagg
     7981 gcgtaatcct tacctctgta tcggtccatg tataaacatt gcgatcgtca taagcgaagc
     8041 gatcgttttt agttagcgga ccgttaactg gaataatttg cacgctcttt gaccgtaatt
     8101 ggcggataaa ataagcatgc tgtttgccgc tattaaatga gcgatcaatg accaggtctg
     8161 gcgtatccag cgggattatc tcaagctggt cttcaccaaa tgtcgataat tttgttacat
     8221 acagccgatc tttatattgc gcaatattgc cagtaatcag atggaattgt tcaggttcaa
     8281 tatctggtag gtctatccac ttgctccact gacggtcgga cgtgagctgt ttacgccagc
     8341 gaacgcgact ttcccctact tcaaaagaga cgtcatcatc tacgggtatc gctttgccag
     8401 acaattgccc aaagggataa cgatgcatac ccttattgtc gcgaaaaacc agcagtgaat
     8461 gaggtatcca gcgaattatc tcaaatgagt ctgcattcgc cgtaaggtga acgccattta
     8521 aataaatatt ctgcccactg cggataagca gatcattgga atacaaattt ctgtttacac
     8581 tcataagacg tggattaata ccccaccatc ttttttctaa tacagtaaca ttactgctgc
     8641 tcccctgacg tcgaccggac aaatagagat ttttactatc caccagcgtg gtagagttac
     8701 cttcgatagc ttttagtgtc gccagatcaa cacgtgacgc gccgctatta ctttcagtgc
     8761 gctgtccgtc aaaatagagg ctatatttat cgaccgcgaa gcggcccata gcctgaaagc
     8821 tggcaatatc gacagtgggt gttcccggtg gattgctaac gattttgcca cgccagagaa
     8881 taacgcggcc gtctgtcacc cagtggtatt cctcattttt tatgcttatt tcatcggtgg
     8941 gtatatagcc ttccaccaac tcccgctccc cggatatatt tgtcacaagg cgcaaatcgg
     9001 ccctgctcat ctccggaaac tgggcaatga ttttcttatc cccccaataa cctttaccgt
     9061 aagacataac accgtgatca ttgtaatagt cgaaccctat ggcctgtaca gcaggcgtca
     9121 gaaatgtgtg tcccaacagg atgagtgcaa ctgtgattat ttgacccttc attttgtcgc
     9181 ctcattgcgc ggggcaggaa taaattcgcc gtcatcagta atttttccgt ccagatatcc
     9241 cgttttgtaa tttacgccat agccatatcc cgaatgttgt tccttccaga ttaagcgaac
     9301 ttgtttcgga tcagcggtgc ggtatcgcca gagctgcgcg ccatcaaagc tgtaaatgta
     9361 gcggttatcg cgggcatacc atctgcgcaa aaagggatgg tcattaagat gggcttcgcg
     9421 ttcatccgga gaacggttat ccagtaagat cagcggtccg tcagattcaa acacatgtaa
     9481 accggaatgg tatggccacc cttcggcacg cgtaagtaaa tagccgtggc gtttcccggc
     9541 gttgaaacgt ttgttgataa taagattggg atcatcaagt ttcacgattt ccagcttgcg
     9601 gtcaccagac tctgtttcga tgatggtata aagcgagtcc tgatactggg cgacagcatc
     9661 actgatgggt tgaaactgct ccggatcgag tccgggtaat tcttcctgtt tacaagcagg
     9721 tcctttacgc cagaacactt tcttttccag cagagaaaaa tccagacagt gctttgttaa
     9781 atcttcatcc cactccagat tttccttgtc gaggacttta cgctgcagcc cgtttttatc
     9841 acgccaggtc aagagtgagc cgggcatcca gcgcacgacg gaaaaggtgt ccgggtcggc
     9901 atcaagctgt tcgccgttga tcaggatttt ggtccgtgta cgagccaggg tatcccaggg
     9961 gccaaatggc atagccacac agggattaaa cgcggtagaa aactttcctc gctgatccca
    10021 tgacttttgt gccagcacac ggaaactatc gggatctgcg aggcgatgcc cattgatata
    10081 caagaaatta gcgtcacgta atatcagccc cagtaagtca ggatcccagg gaggccggaa
    10141 ttccacctga tgcagtgttt tgatatctaa gcgatttccc ccgccattgt tatcggtgcg
    10201 cttgccttcg aaatagagac tgtgtttatc tgcggcaaaa tcaccccacg ccttgaacga
    10261 ggcgacatcg acgggaggtt tgtctggcgg gttttgcaca acttcacccg cgtagaggat
    10321 atagcgacca tcgctatgcc aggagtaatg aggataccag gcctgtgcgc cttcatagag
    10381 gtagtcggtt gtcagatcag tcagaggagg tactttatcc catttgtcta gtgttgtcgg
    10441 gtctattaat aaattgggta aacggcgcaa acttttgtaa ttcacgtcag gaagagaaag
    10501 atttgtttct ttataagggc gcaggtatac cacgcctttt ccctgtttct ggtaaatatt
    10561 atgttcaggc ggatcaagcc gacatcccca tgccgacgat aaaaacaatg aacaagacaa
    10621 caaacaaacc acattgcgat agtgcataaa gccatcctgg cgcgaggtgc cgatcacgaa
    10681 actaccagca gaacataatt cctcgcacgt aagcgtaact tttgcggcaa atcgtcactt
    10741 ttcagaatct ataaaggcca tcgctatttt ctgatagttg ctggggtaaa cttgaggttt
    10801 tcattcacga tataaatacg agctactgcg ccatggtgtg actgatcacg ttaaaatgtg
    10861 ccggattgac gccgggaagg gtgtcaacat gcgactctgc ccaccgttac ctactttatt
    10921 gtctttcgcg tgttgtactt aggtcccacc accggtatct aacattcccc acattattac
    10981 gctttctgcc gaacgtgatt tttatagaaa tctatcaatt cattttccct gtaccataaa
    11041 gtttatcttt cacatcctgc cgaacgcggg tcttgtaccc cccgttgtga agatcttcat
    11101 acggagaaaa agaatcttta ctccacccca gcagcacttt atcgccaggc ataaaatgca
    11161 catgcagaaa atcttctccc cattctcttt tggggagagg taacgtcata ttccgtttct
    11221 ccagacgcat tccagcatta tactgtgtac gagtagtact taatgtccag ataacctctg
    11281 ccgtatcccc actaacatca ccacaacagg ttgttgaacc gccaccattt gcaaaagcat
    11341 tcgcgcccgc cacgccgtta acactgaatc ccagtatcgg gcggtcaatt tcactgtgga
    11401 tttccagaat cactccacct cttggtcctc cccatactgc ctgtcctata ttccacaaca
    11461 cgaccagtac cggaacgaca attaacgtcc gccataccca gcgcccccac cgggcataac
    11521 cgcgcgtaag cttttggtca aatttttcca gttgggtaaa tagccccatc agattttatt
    11581 ccctcttatc accaggttgg caaagttgta tgtcactggc gactaaggtc agacaacaaa
    11641 attgcatggt gcgccactct tttaaaagct ccatgccgac gataagaaca acgaacaagc
    11701 caacaaacaa actacattgc gatagtgcat agagccatcc tggcgcgagg tgccgatcac
    11761 gaaactacca gcaaaacata aatccccacg agtaagcgtt atactcgcag catttcctca
    11821 cttttcagac ttcataaaga gtcgctaaac gcttgctttt acgtcttctc ctgcgatgat
    11881 agaaagcaga aagcgatgaa ctttacaggc aatccataat gaaaaacgtg tttaaggcac
    11941 tcactgtatt aattactctc ttcagcctga cgggctgcgg tctgaaaggt ccgctctatt
    12001 tcccgcctgc agataaaaac gcaccgccgc cgaccaaacc ggtagagacg caaacgcaat
    12061 ccacggtgcc ggataaaaac gatcgcgcca ctggcgatgg tccatcccag gtgaattact
    12121 aaaagtcagt ttctgtaccc gcgtgattgg agtaaatgat gcagttctcg aaaatgcatg
    12181 gccttggcaa cgattttatg gtcgtcgacg cggtaacgca gaatgtcttt ttttcaccgg
    12241 agctgattcg tcgcctggct gatcggcacc tgggggtagg gtttgaccaa ctgctggtgg
    12301 ttgagccgcc gtatgatcct gaactggatt ttcactatcg cattttcaat gctgatggca
    12361 gtgaagtggc gcagtgcggc aacggtgcgc gctgctttgc ccgttttgtg cgtctgaaag
    12421 gactgaccaa taagcgtgat atccgcgtca gcaccgccaa cgggcggatg gttctgaccg
    12481 tcaccgatga tgatctggtc cgcgtaaata tgggcgaacc caacttcgaa ccttccgccg
    12541 tgccgtttcg cgctaacaaa gcggaaaaga cctatattat gcgcgccgcc gagcagacaa
    12601 tcttatgcgg cgtggtgtcg atgggaaatc cgcattgcgt gattcaggtc gatgatgtcg
    12661 ataccgcggc ggtagaaacg cttggtcctg ttctggaaag ccacgagcgt tttccggagc
    12721 gcgccaatat cggttttatg caagtggtta agcgcgagca tattcgttta cgcgtttatg
    12781 agcgtggggc aggagaaacc caggcctgcg gcagcggcgc gtgtgcggcg gttgcagtag
    12841 ggattcagca aggtttgctg gccgaagaag tacgcgtgga actccccggc ggtcgtcttg
    12901 atatcgcctg gaaaggtccg ggtcacccgt tatatatgac tggcccggcg gtacatgtct
    12961 acgacggatt tattcatcta tgaagcaacc aggggaagaa ctgcaggaaa cactcacgga
    13021 gcttgatgac cgggcggttg tcgattatct gattaaaaat cctgagtttt ttatccgtaa
    13081 tgcgcgcgca gtagaagcga tacgtgtgcc gcatccggta cgcggcaccg tttcgttggt
    13141 cgagtggcac atggcccgcg cacgtaatca tattcatgtt ctggaagaga acatggcgct
    13201 gttgatggaa caggctatcg ccaacgaagg cctgttttat cgcctactct acctgcagcg
    13261 cagtctcacc gccgccagca gtctcgacga tatgctgatg cgctttcacc gctgggcgcg
    13321 cgatctcggc ctggcaggtg cgagtctgcg cctgtttccg gatcgctggc gcttaggtgc
    13381 gccgtcgaac cacactcatc tggcattaag ccgtcagtct ttcgaaccgc tgcgtattca
    13441 gcgtttgggg caggaacagc actatcttgg gccgcttaac ggaccagagc tgctggtggt
    13501 gctaccggaa gcgaaagcgg tgggatcggt ggcgatgtcg atgctgggaa gcgatgctga
    13561 tttgggtgtc gtgctgttta ccagtcgcga tgccagtcac tatcaacaag ggcaaggaac
    13621 gcagttactt catgaaattg cgctgatgtt gccggagctt ctggagcgtt ggattgaacg
    13681 cgtatgaccg atttacacac cgatgtagaa cgctacctac gttatctgag cgtggagcgc
    13741 cagcttagcc cgataaccct gcttaactac cagcgtcagc ttgaggcgat catcaatttt
    13801 gccagcgaaa acggcctgca aagctggcag caatgtgatg tgacgatggt gcgcaatttt
    13861 gctgtacgca gtcgccgtaa agggctggga gcagcaagtc tggcgttacg gctttctgcg
    13921 ctacgtagct tttttgactg gctggtcagc cagaacgaac tcaaagctaa cccggcgaaa
    13981 ggtgtttcgg caccgaaagc gccgcgtcat ctgccgaaaa acatcgacgt cgacgatatg
    14041 aatcggctgc tggatattga tatcaacgat cccctcgctg tacgcgaccg tgcaatgctg
    14101 gaagtgatgt acggcgcggg tctgcgtctt tctgagctgg tggggctgga tattaaacac
    14161 ctcgacctgg agtctggtga agtgtgggtt atggggaaag gcagcaaaga gcgccgcctg
    14221 ccgattggtc gcaacgctgt ggcgtggatt gagcactggc ttgatttgcg cgacctgttt
    14281 ggtagcgaag acgacgcgct ttttctgtcg aaactgggca agcgtatctc cgcgcgtaat
    14341 gtgcagaaac gctttgccga atggggcata aaacaagggc tgaataatca cgttcaccca
    14401 cataaattac gtcactcgtt cgctacgcat atgctggagt cgagcggcga tcttcgtggt
    14461 gtgcaggagc tactgggtca tgccaacctc tccaccacgc aaatctatac tcatcttgat
    14521 tttcaacacc ttgcctcggt gtacgatgcg gcgcatccac gcgccaaacg ggggaaataa
    14581 tgcgttttta ccggcctttg gggcgcatct cggcgctcac ctttgacctg gatgataccc
    14641 tttacgataa ccgtccggtg attttgcgca ccgagcgaga ggcgcttacc tttgtgcaaa
    14701 attatcatcc ggcgctgcgc agcttccaga atgaagatct gcaacgcctg cgccaggcgg
    14761 tacgggaagt ggaacccgag atttatcacg acgtgacgcg ctggcgtttt cgttcgattg
    14821 aacaagcgat gctcgacgcc gggctgagtg ccgaagaagc cagtgcaggc gcacacgcag
    14881 caatgatcaa ctttgccaaa tggcgcagcc gaatcgacgt cccgcagcaa actcacgaca
    14941 ccttaaaaca gctggcgaag aaatggccgc tggtggcgat caccaacggt aacgcccagc
    15001 cggaactgtt tggtttgggg gattattttg agtttgtgct gcgcgctggc ccgcacgggc
    15061 gctcaaaacc gttcagcgat atgtactttc tggctgcgga aaaactcaac gtgccgatcg
    15121 gcgagatctt acatgttggg gacgatctca ccactgacgt gggtggggca attcgcagcg
    15181 gaatgcaggc ctgttggatc agaccggaaa atggcgatct gatgcaaacc tgggacagcc
    15241 gtttactgcc gcatctggaa atttcccggt tggcatctct gacctcgctg atataatcag
    15301 caaatctgta tatataccca gctttttggc ggagggcgtt gcgcttctcc gcccaaccta
    15361 tttttacgcg gcggtgccaa tggacgtttc ttacctgctc gacagcctta atgacaaaca
    15421 gcgcgaagcg gtggccgcgc cacgcagcaa ccttctggtg ctggcgggcg cgggcagtgg
    15481 taagacgcgc gtactggtgc atcgtatcgc ctggctgatg agcgtggaaa actgctcgcc
    15541 atactcgatt atggcggtga cgtttaccaa caaagcggcg gcggagatgc gtcatcgtat
    15601 cgggcagctg atgggcacca gccagggcgg catgtgggtc ggcaccttcc acgggctggc
    15661 gcaccgtctg ctgcgtgcgc accatatgga cgccaatctg ccgcaggatt ttcagatcct
    15721 cgacagcgag gaccagctac gcctgctcaa acgcctaatc aaggcgatga acctcgacga
    15781 gaagcagtgg ccgccgcgcc aggcaatgtg gtacatcaac agccagaaag atgaaggcct
    15841 gcgtccacat catattcaaa gctacggtaa tccggtggag cagacctggc agaaggtgta
    15901 tcaggcgtat caggaagcgt gtgaccgcgc gggcctggtg gacttcgccg agctgctgct
    15961 acgtgctcac gagttgtggc ttaacaagcc gcatatcctt cagcactatc gcgaacggtt
    16021 taccaatatc ctggtggacg aattccagga taccaacaac attcagtatg cgtggatccg
    16081 cctgctggcg ggcgataccg gcaaagtgat gatcgttggc gatgacgatc agtcaatcta
    16141 cggctggcgt ggagcgcagg tggagaatat tcagcgtttc ctcaatgatt tccccggtgc
    16201 tgaaactatc cgtctggagc agaactaccg ctctaccagc aatattctga gcgccgcgaa
    16261 cgccctgatt gaaaacaaca acgggcgttt gggtaaaaaa ctgtggaccg atggcgcgga
    16321 cggtgagcct atttccctct attgcgcttt taacgaactc gatgaagcgc gttttgtggt
    16381 taaccgcatc aaaacctggc aggacaacgg cggggcgctt gccgagtgcg ccattctcta
    16441 ccgcagtaac gcccagtcgc gtgtgctgga agaagcactg ttgcaggcga gtatgccgta
    16501 ccgtatttac ggcgggatgc gcttcttcga acgccaggaa atcaaagatg cgctctcgta
    16561 tctgcgcctg attgccaacc gtaacgacga cgcggccttt gagcgtgtgg tgaatacgcc
    16621 aacgcggggt attggtgacc ggacgctgga cgtggtacgt cagacatcgc gcgatcgcca
    16681 gttaacactc tggcaggcat gtcgtgaact gttgcaggaa aaagccctcg ccggacgtgc
    16741 tgccagcgcc ttacagcggt ttatggaact gatcgacgcc ttagcgcagg aaactgccga
    16801 tatgccgctg catgtacaga ctgaccgggt aattaaagac tccggcctgc gtaccatgta
    16861 cgagcaggag aagggcgaaa aaggtcagac gcgtatcgaa aacttagagg aactggtgac
    16921 ggcaacgcgc cagttcagct acaacgaaga agacgaagat ttaatgccgc tgcaggcgtt
    16981 cctatcccat gcggcgctgg aagcaggcga agggcaggcg gatacctggc aagatgcggt
    17041 acaactgatg accatgcact cggcgaaagg tctggagttc ccgcaggtgt ttatcgtcgg
    17101 tatggaagag ggcatgttcc caagccagat gtcgctggat gaaggcgggc gtctggaaga
    17161 agaacgccgt ctggcctacg ttggcgtaac ccgcgcgatg cagaaactga cgctgaccta
    17221 cgcggaaact cgccgtctgt atggcaaaga ggtttaccat cgcccgtcgc gctttattgg
    17281 cgagctgccg gaagagtgtg tggaagaggt gcgcctgcgc gccacggtaa gccgcccggt
    17341 cagccatcag cggatgggta cgccgatggt cgagaacgac agcggctaca agctcggcca
    17401 gcgcgtacgc cacgctaagt ttggtgaagg caccattgtc aatatggaag gcagcggtga
    17461 gcatagccgt ttgcaggtgg catttcaggg ccagggtatt aaatggctgg tggcggctta
    17521 tgcccggctg gagtcggtgt aacgttgccg gatgcggtgc tgcgcacctt acttggccta
    17581 aaaaatcatt cagattcaat aaattgcaac gtcatgtagg ccggataggg cgtttacgcc
    17641 gcatccggca tctgcgccat cttcagtatc tgacacaaaa ctatcgtttt aacctttccg
    17701 ctctacggaa atcatggtga caaaaggata gcgttgccac gggattgccc cgcctttcat
    17761 atacatatgt gaaatcgtgc catcaagata aagcagctgc tcaacgttca gtttcgcttt
    17821 ggcataacag gcaaaatcat aaaaatttgt cgcctgctgg ctcaacaaaa acacggcatt
    17881 cccatgttta ttaatcccaa caccgttacg aattttacgt gaggcgacgt tgggatgaat
    17941 acgcggatta attacactgt tttccatcaa cattggccct gactgcaccg caaactgaat
    18001 ctctttactg gttttgaagg catccagacg aacgatgccg actttatctc ccgcgacata
    18061 aaacacgccg ccaggacgga taaagaaatt cccttcacct gaagcgagat ttaacgccac
    18121 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
    18181 gccgttcatc gccatctgca cctgaccctg actattcata tccgccagca gagcatgtaa
    18241 agttccccac gcttcgccat tggctttttg ccagtacatt ttcacccgct ctgtttgagg
    18301 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggc
    18361 aacagcaaac aagggaagta aggtgagggt gagaaaaatc cgtttgagat tcaaggtgat
    18421 cattccttta ccaatgagta gccgatgcgc cattataggt cctggatgtt ggattttttt
    18481 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
    18541 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
    18601 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
    18661 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
    18721 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
    18781 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
    18841 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
    18901 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
    18961 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
    19021 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
    19081 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
    19141 cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac
    19201 ggtaacgcct acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat
    19261 gaaattgaaa atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat
    19321 cagggcgatg agtacgacga ggcgctctcc acgctggcgg aactggaaga tatcggctgg
    19381 aaagttcgcc tgtgtctgat ggatacccag cgcgcgctca acttcctggt acgcaaggcg
    19441 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgagatat cgaatccctg
    19501 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcgatgggc
    19561 tttatcaaca tcgagcagaa ccgcatcatc aaaatcttct cggtggtatc cgtggtgttc
    19621 ctgccaccga cgctggtggc ttccagctac gggatgaact ttgaatttat gccagagttg
    19681 aagtggagtt tcggctaccc aggcgcgatt atctttatga tcctcgcggg cctggcaccg
    19741 tatctgtact ttaagcggaa gaactggttg taatatgagt gccggataaa gctatttttt
    19801 tatccggctt atttctaaaa attgtctttt ttaaagtcat gcggattgaa aactcgctct
    19861 attttatttt cttgtgttaa ttgatactca gtaaaatcgt taataccaat atgagtgtta
    19921 cgataagggt attcattaat aaccattaag taatttttca cgatgcaatt atcattgctc
    19981 ttaattgtcg ggaaacagta gagatcttca tacggccctt cgatatattt ttttatagtc
    20041 agtgaagcca ttaccgatat caatgacata ccaggtatgc caactacctt ctggtagggg
    20101 atgttgattt ttaatttcat tctgatgctt cagataccaa tttattttat cgctgtcagt
    20161 ccatggcaga tgatcaacga ccagaaagac tgaattgcca gcatcagagt atgtatacaa
    20221 aatctgtaca ggacgcaatg tccataaatt atagatgcag catagaagaa ataaacataa
    20281 tataattttc tggagtttgc ttttcataga taattttcga ttctttcgat agtgttaaaa
    20341 ttggtgaaaa aaggtttgaa agcaaagtct ttatgccgtt gtaaaaagaa ccatatgcga
    20401 aaaaatctat atttgctgta agtcttgttt ttgatatcag ttacatcaag accgaagtgg
    20461 tcctgtgcta ca
//
LOCUS       DAZFJF010000081        20374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFJF010000081 DAZFJF010000000
VERSION     DAZFJF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.081"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(66..476)
                     /gene="ybcV"
                     /locus_tag="ACHKAU_003240"
     CDS             complement(66..476)
                     /gene="ybcV"
                     /locus_tag="ACHKAU_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO5532247.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            777..983
                     /locus_tag="ACHKAU_003241"
     CDS             777..983
                     /locus_tag="ACHKAU_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532248.1"
                     /translation="MNKEQSADEVSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1556..2083
                     /locus_tag="ACHKAU_003242"
     CDS             1556..2083
                     /locus_tag="ACHKAU_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO5532249.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATV
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            2092..4191
                     /locus_tag="ACHKAU_003243"
     CDS             2092..4191
                     /locus_tag="ACHKAU_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5532250.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            4188..4400
                     /locus_tag="ACHKAU_003244"
     CDS             4188..4400
                     /locus_tag="ACHKAU_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532251.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            4400..5908
                     /locus_tag="ACHKAU_003245"
     CDS             4400..5908
                     /locus_tag="ACHKAU_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5532252.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5853..7880
                     /locus_tag="ACHKAU_003246"
     CDS             5853..7880
                     /locus_tag="ACHKAU_003246"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO5532253.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7967..8290
                     /locus_tag="ACHKAU_003247"
     CDS             7967..8290
                     /locus_tag="ACHKAU_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO5532254.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSEPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            8283..8558
                     /locus_tag="ACHKAU_003248"
     CDS             8283..8558
                     /locus_tag="ACHKAU_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO5532255.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMIVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            8570..9148
                     /locus_tag="ACHKAU_003249"
     CDS             8570..9148
                     /locus_tag="ACHKAU_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5532256.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTVRIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGSLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            9145..9546
                     /locus_tag="ACHKAU_003250"
     CDS             9145..9546
                     /locus_tag="ACHKAU_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5532257.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            9557..10300
                     /locus_tag="ACHKAU_003251"
     CDS             9557..10300
                     /locus_tag="ACHKAU_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5532258.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            10361..10747
                     /locus_tag="ACHKAU_003252"
     CDS             10361..10747
                     /locus_tag="ACHKAU_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5532259.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            10756..11085
                     /locus_tag="ACHKAU_003253"
     CDS             10756..11085
                     /locus_tag="ACHKAU_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5532260.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            11057..14122
                     /locus_tag="ACHKAU_003254"
     CDS             11057..14122
                     /locus_tag="ACHKAU_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5532261.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARVAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            14122..14451
                     /locus_tag="ACHKAU_003255"
     CDS             14122..14451
                     /locus_tag="ACHKAU_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5532262.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            14461..15159
                     /locus_tag="ACHKAU_003256"
     CDS             14461..15159
                     /locus_tag="ACHKAU_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5532263.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15164..15907
                     /locus_tag="ACHKAU_003257"
     CDS             15164..15907
                     /locus_tag="ACHKAU_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5532264.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            15805..16452
                     /locus_tag="ACHKAU_003258"
     CDS             15805..16452
                     /locus_tag="ACHKAU_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5532265.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16513..19926
                     /locus_tag="ACHKAU_003259"
     CDS             16513..19926
                     /locus_tag="ACHKAU_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5532266.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQNRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPEGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDAASVSFRIAAPAAPSRIELTPGYFQITATPHLAIYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            19997..>20374
                     /locus_tag="ACHKAU_003260"
     CDS             19997..>20374
                     /locus_tag="ACHKAU_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5532267.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVST"
BASE COUNT         4927 a         4731 c         6113 g         4603 t
ORIGIN      
        1 tgttgatggt ttttattaaa ccaatgcagt ccggctcagg agtgagagaa gccggacgtt
       61 atggtttagc gtggtaagat ctgtgtagtt ttctggatgc tttcagtaaa tagtaatgag
      121 ttatcaaagg catagtaata tctttggtgt tcctggatat ttgtaaccca tcggaaaact
      181 cctgctttag caagattttc cctgtattgt tgaaatgtga tttcttttga tttcaactta
      241 ttataggagg tctctataag atgtttgttt ctggagaatt taacatttac aacctttttg
      301 agtcctttta ctaacactat gttgtcgttt tctaacacaa tgtgaatatt atctgtggct
      361 aaatagtaaa tataaagtga gacattgtga cgttttagct cagaataaaa taattcacag
      421 tttaaatctt tacgcacttg atcgaatatt tctttaaaaa tggcagcctg agccattggt
      481 aaaccttcca tgtgatacga tggcgcgtag ttagcattat cgtgtttatt gtttcaatct
      541 ggtctgacct ctttgtgttt tgttgatgat ttatgtcaaa tattaagcct gtttttaatg
      601 aatagtgttg attgcgtaac aaaattgagc cttgctggca tccaggaggg atatgcaacc
      661 gacagatgta tgtaaggtcg atgtactcaa actttcatac ttttcctctt ttatgcagta
      721 agatttgaag taatatttta accgctagat gacgagcaaa cgcatggagc gacaaaatga
      781 ataaagaaca atctgctgat gaagtctcgt tggatctgat tcgtgtaaaa aatatgctta
      841 atagcaccat ttctatgagt tacccggatg ttgtaattgc atgtatagaa catcaggtgt
      901 ctctggaagc attcagggca attgaggcag cgttggtgaa gcacgataag aattcgaagg
      961 attattccct ggtggttgac tgagcaccat aactgctaat cattcaaact atttcacctg
     1021 tgacagagtc aatatcgcat tctgtcactg tcaggctaat acagagctgc aattcaacta
     1081 ctgcaatgtc ctcgtaatta ggtgaattta caatatcgtc ctgttcggat gccggttgca
     1141 ttgctgaaga tgaggcattt atggttcgca tattttcccc tcatgctcgt cagtcctgtg
     1201 cgtaggaaga aacaggacac tcacactaat ttgtgtgggc atgctgtgat gtccttctga
     1261 attattccta tgccattatg taaagcgctg tatcagatgc tcatcacggc tgtcaggctg
     1321 tcaggctgtc gggtcctccc ggtgggggcc cctgccacgg ggcgggagcg tcgcggaaaa
     1381 aggctagttt ttgcatttcc atggcggcgg cagcatgttt ggtaatttat tgataattaa
     1441 aagttatttc tcttttcacc tgtacaatat ttctttctcc ctgtcattag accagtttgc
     1501 aattaattga aatatataaa taaacatgat tttcacctgc cagatggagt tgcttatgtc
     1561 aaatgtgagc gggatcggtg atgcttatta ctggagtgtt tttaaaatcg ctgaggcctt
     1621 tgggcttcac cgggacacag taaaaaaacg gctcctcgcg gctaacactc ctgtggctgc
     1681 gactgtcagg gggaaccccg tttacgccct gcagcatgtc gggccagccc tgtttagtgt
     1741 gaagcatgag gcagcagact ctgttcatga tccatcccgt atggagccga aagagagaaa
     1801 ggactggtac caatctgaaa atgaaaggat caagctggaa aaggagcagc gaaaactcat
     1861 ccccgttgat gaagtagtca tcgtctattc gtccatgaga aaggctgtcg tccaggttct
     1921 ggagacaatt ccggatgttc ttgaacgcga ttgcgccctg actcctcagg ccgtcggcgt
     1981 tgtacagcag gccattgatg acctgcgata cactcttcag gaaaaatcct acgaggcttg
     2041 tgctgctgaa ttaattcctg atgaggaagg agagagtctc taggaggaat aatgggtttt
     2101 tcatcagccc gaaatttggg aagggacata tcggcaggat tttccccacc acgtcgcatg
     2161 ccgatttcgg aggctgttaa aaaattcatg cgtgttccca agggggctgg taactcggtg
     2221 ccatgggatc ctgaactgac accctacatc attgagccca tgaactgcct ggcatcgcgt
     2281 gaatacgatg cggtgatttt tgttggtcct gcgcgaacag ggaagaccat tggtctgatc
     2341 gatggatgga ttgtctatac catcgtttgc gatccttcgg acatgctcgt tgtgcagatg
     2401 accgaagata aggcccgcga gcattctaaa aagcgcctcg acagaacgtt cagaagcagt
     2461 gcggcggtaa agaaaagaat gagtccacgt cgtaacgaca ataatgtcca tgataagacg
     2521 ttcagggatg gctcgttcct taaaattggt tggccctcgg tcaacattat gtcgtcgtcg
     2581 gattaccggt ttgtcgcctt aaccgattac gaccgttttc cggagaatat cgacagcgag
     2641 ggtgatggtt tctccctggc ctcaaaacgt accaccacat ttatgtccgc tgggatgact
     2701 ctggtggaga gctcgccggg acgtgacatc tgcgacagca aatggcgacg taagtcgcct
     2761 catgaagcgc caccgacgac tggtattctt tccctttaca atcgtggtga ccgccgccgc
     2821 tggtactggt catgtccgca ctgcggtgaa tattttcagc cagccatgga tgccatgacc
     2881 ggctaccgta atgaaccgga tccctttaaa gccagtgagg cggcgtatct actttgcccg
     2941 cactgcagcg gcattatcac tgcggagaaa aagcgtgagc tcaatagtgc aggagtctgg
     3001 ttgcgtgaag gtcaggtcat tgatcgtaac ggcaacgttt ccggtgaacc gcgccgctcc
     3061 cgtatcgcca gtttctggat ggaagggcca gctgctgcgt atcagacctg ggcgcaactg
     3121 gtttacaaat tactgactgc agaacaggag tatgaagcga caggaagcga agaaacactc
     3181 agggcggtta tcaacaccga ctggggattg ccttatcttc ctcgtgccag catggagcaa
     3241 cgaaaaagtg aactgcttga gcagcgggca gagccagttc cttcccgcag tgtgccggat
     3301 ggcgttaatt tccttgtggc gacagtggat gtgcaggcgg gacgtcatcg ccgttttgtg
     3361 gttcaggtaa cgggctatgg cagtcgtggc gaacgctgga ttattgatcg ttacaacatc
     3421 acgcagtcat tgcgcagtga ctgcgacggg gagagccagc gaattgatcc ggccagctat
     3481 ccggaagact gggatgtcct gctgacggat gtttttcata aaagctggcc gctggcctcc
     3541 gatccttctc aacaaatgcg actgatggca atggcggtgg actccggcgg tgaagacggg
     3601 gtcactgata atgcctataa attctggcgt cgttgccgtc gtgatggcct tggtaaacgt
     3661 atttacctgt ttaagggtga cagcatccgg cgcgcaaaac tgatcagccg tacattccct
     3721 gataacaccg gacgaacggg ccgccgggcg caggccgcag gtgatgtgcc gctctggctt
     3781 cttcagacgg atgccctgaa agaccgggtg aataacgcgt tatggcgtga ctcgccaggt
     3841 ccaggctatg tgcatttccc tgactggctg gggagctggt tttacgacga actgacgtat
     3901 gaagagcgga gcagtgacgg gaaatggagt aagccgggtc gcggtgccaa cgaagctttt
     3961 gacctgatgg tgtatgccga ggctctggtc attctgcatg gatacgaaaa gatccgctgg
     4021 ccggatgcac cggagtgggc gagccgggag acctggctgg agtgtgtcca ggacagtacc
     4081 gaaccgtcat cctcaccgga accggtatcc acgcctgtta aaaaacaaaa acggaagaaa
     4141 acagtaactg acgatgttaa cccctggctg acttccggag gatggttatg aaccagaatg
     4201 atatcgaagc catgattcag cgttatacgg aagctgaaat ggcggtgctg gacggaaaat
     4261 ccgtcacctt taatggtcag cagatgacca tggaaaactt atctgagatc cggcagggac
     4321 ggcaggagtg ggagcgccgc cttgcggctc tgattacacg acgacggggg catcccgggt
     4381 accggctggc gaggttctga tggcaattct tgatgatgtg attggcgttt tttcaccagg
     4441 atggaaagcg gcaaggctgc gttcccgtgc ggtgatccag gcttatgagg ccgtaaaaac
     4501 gacgcggaca cacaaagccc gacgggagaa ccgaactgcc gaccagttaa gccagtacgg
     4561 ggccgtgtcg ttacgtgagc aggcccgtta ccttgataac aaccacgatc tggttattgg
     4621 tgtatttgac aagctggaag aacgggtggt ggggaaaaac gggattattg tcgagccaca
     4681 tccggtatta cgcaatgggg ccattgcccg tgacctggct gcggagattc gcacccgatg
     4741 gagtgaatgg tctgtcagcc cggaagtcac cgggcagttt acccgtccga tgctggaacg
     4801 tctgatgctt cgtacctggc tgcgcgatgg tgaggtgttt gcccagatgg tttccgggcg
     4861 cataaacagc ctggcgcctt ctgccggtgt tcatttctgg ctggaggcgc tcgagccgga
     4921 ctttattccc atgactagtg atgagagcaa caggctgaat cagggcgtgt ttgttgatga
     4981 ctgggggcgt cccgaaaaat atctggtgta taaaagccgt cccgtatccg gacggcagat
     5041 ggaaaccaaa gaagtggatg cagagcgaat gctgcatctt aaatttgttc gccgtctgca
     5101 ccagatgcgc gggacgtctt tgttgtccgg tgtgctgatc cgcctcagtg ccctgaaaga
     5161 atatgaagat tctgagctga ctgcagcaag gatcgccgct gctctgggga tgtacatccg
     5221 gaaaggcgac gggcagagct atgaaccgga tggtaatggc agaaaggaga atgaacgtga
     5281 gcttaccatt cagccgggca ttatttacga cgatctgaaa cccggcgaag aaatcggaat
     5341 ggtgaagtcg gatcgcccca atcctaacct tgaaactttt cgtaatggtc agttgcgtgc
     5401 cgtggcggcg ggcagtcgtc tgagtttttc cagtacagcg cgcaactata acggcactta
     5461 cagcgcccag cgtcaggagc tggttgaatc cactgatggc tacctgatcc tgcaggactg
     5521 gtttattggt gccgtcaccc gcccgatgta tcgtgcatgg ctgaaacagg ctgtggcatc
     5581 cggtgttatc aggctacccc gcgatcttga ccgttcttca ctgtataccg cggtgtattc
     5641 cggaccagtg atgccgtgga ttgaccctgt taaggaggct gaggcctgga aaatccagat
     5701 tcgtggtgga gcggcgacag aatcagactg ggtacgtgct ggtggtcgta atccggatga
     5761 tgtcaaacgt cggcgcaagg ccgaaattga tgaaaaccgc aagctggatc tggtatttga
     5821 taccgatccg gccagtgata aaggaggcag tagtgccgca acgaaacgac aggagccgca
     5881 gcacaccgac gaccagtccg aagaataatt cctggttcag gatgcaggct ggtcaccaga
     5941 gtgacgcgga tatttatatt tatgacgaga ttggtttctg gggtgttaca gcgaagcagt
     6001 ttatcagtga tctgaatgca ctgggcgata tcacccacat taatctccat attaattcac
     6061 cgggtggcga tgtctttgaa ggcatcgcca tttttaatgc gctgaaaaca catggtgcgt
     6121 ccattaccgt ttatgtcgac ggtgtggcgg cgtcaatggc gtcggtcatt gcgatggtag
     6181 gaaacccggt cattatgccg gaaaactcct tcatgatgat tcataaacca tttggcttta
     6241 cgggcggtga tgcggaggac atgcgcacct atgccgacct gctcgataaa gttgaggcgg
     6301 ttctgttacc cgcttatgca cagaaaaccg ggaaaaccac cgatgaaatt gctgccatgc
     6361 tggcggatga gacctggatg tccggtgccg aatgtctggc acagggattt gctgatcagg
     6421 tgacaccagc cgttaaggca atggcatgta ttcagtcaaa acgtacagag gaatttaaaa
     6481 agatgccgga atccattcga aatatgatta ctccgccacg caacagtgct ccacgcgtac
     6541 aggatgatgg acctgcagcc tcccggacgc cagtgcaggc agcagcaccc gtggtggatg
     6601 aaaacagcat ccgtgcgcag gtcctggcag agcaaaaagc gcgtgtaaac ggtattaatg
     6661 atctgtttgc catgtttggc gggcgttatc agtcgttgca ggctcagtgt cttgccgatc
     6721 ctgaatgttc gctggagcag gcccgcgaaa agctgttgaa cgagatgggg cgcgagtcca
     6781 cgccatccaa taaaaatacc ccggctcata tttatgccgg taacggtaat tttgtggggg
     6841 acgggatccg ccaggcgctg atggcgcgcg ccgggtttga aaaaactgaa cgtgataatg
     6901 tctacaacgg gatgaccctg cgtgaatatg cccgtatgtc actgactgaa cgtggtattg
     6961 gtgtttccag ttataacccg atgcagatgg tcggtgcggc gttcacacac agtacgtctg
     7021 acttcggtaa tattctgctg gatgttgcga acaaagccat tctgcagggc tgggaagatg
     7081 cccctgaaac ctatgaacag tggacgcgga aaggtcagtt gtctgatttt aaaattgccc
     7141 atcgtgtggg tatggggggc ttcagtgctc tgcgtcaggt gcgtgaaggg gcagaatata
     7201 aatacgtcac caccggagat aaacaggcca ctattgcact ggcgacctat ggcgagctgt
     7261 tcagtatcac ccgtcaggcc attatcaatg atgatctgaa tatgctgacc gatgtcccga
     7321 tgaagctggg ccgtgcggcg aaatccacta ttgccgatct ggtttatgcc attctgacgt
     7381 ctaacccgaa aatctccaca gataatgtaa gtctgttcga taaagcgaaa catgcaaacg
     7441 tactggagag cgcggcaatg gacgtggcat cgctggataa agcccgccag ttgatgcgcg
     7501 ttcagaaaga gggtgagcgt catctgaata ttcgtcctgc gttcgtactg gtaccgacgg
     7561 cgatggagtc tgttgctaac caggtcattc gctcctcaag tgtcaagggg gctgacatta
     7621 acgccggtat tattaacccg gtgaaagatt ttgcgaccgt tattgcagag cctcgtcttg
     7681 atgataacag ccagaccacc ttctacctgg ctgcgtccaa aggctccgat acgattgaag
     7741 tggcttatct caacggtgtg gatacgccat atattgatca gatggagggc ttcagtgtgg
     7801 atggcgtgac aacgaaagtg cgtattgacg ctggtgttgc gccagttgat caccgcggcc
     7861 tggtgaaatg tacggcgtaa acgtcgcaga caacaactct gatggcccgt aagggctttt
     7921 tttgtacctg aaatcagccc ctgaacgggg ctgtgcggag acagttatgg caaagaattt
     7981 tgtagaagaa ggaaaaacgg tggcgattgt tgccagtgca gccatcagca gcggagatct
     8041 ggtgcaggtg ggggatgttt ttgcggtggc gctgaccgat attccacagg gagaaacagg
     8101 cgacggcata accgaaggtg tgtttatgct gcctaagctg aaaacggatg acatgaaaac
     8161 gggtaagaag gtttatctga agtcaggaaa agttcagctg actaacagcg gctctgagcc
     8221 gctggtcggg gttgtctggg cagatgccgg aaccagtgca gaagaagtgc cggtaaaact
     8281 caatgtctga tcccttttcc cggctggcag cgcgtatgga tgcgatcacg gtcagaaaga
     8341 tgggaaagac agcctcgatt aatgatgtcg atatgattgt gatcccggga gaaacactgg
     8401 cagagctgaa tgctctgtcc ggacctgcgg tctctctggt ggtgttttct tcgggatacc
     8461 gcccacggcg cggggatcgc gttgtttatg acggacaaca atggacggtc acacggcatg
     8521 aacgttttaa cggtaagcca atgatcttta ttgagtaaag aggtgtggga tgaaggggct
     8581 tgagaatgcc atccgcaatc tgaacagcct tgatacccgt atggtgccac aggccagcgc
     8641 atgggcgata aaccgtgtgg cacagaaagc ggtctcggtt gccacccggc aggttgccgg
     8701 gaataccgtt gcgggagata accaggtgaa agggatcccc ctgaaactgg tacgtcagcg
     8761 tgtccgggtg tttaaagcca gtccgtcagg aaaaatgacg gtcaggatcc gcgttaaccg
     8821 gggcaatctg cccgccatta agctggggac agcccgggtc agactggccc ggcgtggtgg
     8881 aaaactgcag taccgtggca gtgtgctgaa ggtgggtaaa tatcttttcc gggatgcgtt
     8941 tattcagcaa ctggcgaatg gtcgctggca tgtgatgcga cgtattgatg gcaaaaatcg
     9001 ttaccccatt gatgtggtga aaatcccgct gtccggatcg ctgacacagg catttgaaga
     9061 tgcccgcgac cacatcattg ctgcggaaat gccgaaacag ctggggtatg cactaaaaca
     9121 acaactgagg ttatggctga cccgatgaac cgacatacac aaatccgcca ggccgtactg
     9181 gcacgccttc gggaacagtg tggagacagc gccacgtttt ttgacgggct tccggcattt
     9241 attgatgcgc aggaactgcc tgccgtggcg gtgtggctga gtgatgctca gtacaccgga
     9301 aaaatgacgg atgaagatga ctggcaggct gttctgcata ttgccgtctt catccgggca
     9361 caggcaccgg attcagagct ggatatgtgg atggagagca ccattttccc tgccctgaat
     9421 gatataccgg cactttccgg actcatcgac accctgatcc cactcggttt taactatcaa
     9481 cgtgataatg agatggccac ctgggcgatg gcggaaatca cgtaccagat cacgtacacg
     9541 aattaaggag gtggtaatga ccacaccaaa tccactggca aagacgaaag gtgcggggac
     9601 gacgttctgg atgtataccg gcaacggcga tgcgtttgcg aaccctttgt cggacactga
     9661 ctggctgcgt cttgcgatgg tgaaggatct gcaacctggc gaaatgaccg ctgatgcaga
     9721 agatgacact tatctcgatg atgaagatgc agactggaaa acgacaaccc aggggcagaa
     9781 atccgttggt gatacttcgg cgacgctggc ctggcgtccg ggtgacagcg ggcagaaaaa
     9841 actggttcag ttgttcgact ccggtgaagt ctgcgcgttt cgtatcaaat atcccaacgg
     9901 cactgttgat gttttccgtg gctggctgag ctcactgggt aaaaccattg cctcaaaaga
     9961 cgtgatgacc cgcactgtga aaatcagcgg tgtggggcgt ccgtatctgg cagaggaagg
    10021 cactgaaacc gtgagcgtta ccgggctgac agtatcaccg gcgtctgcca gtgtaaaagt
    10081 gggagcaacc accacgctga cctttacagt aaaacctgac ggagccagtg acaaagcgat
    10141 cagtgtgcat tcgtcagatc cacagactgc cacggttacc ctgaacggac ttgtggccac
    10201 ggtaaaaggc gtgaagcagg gcagtgtcag cattgtgggc atgaccgctg acgggaattt
    10261 tgtggctgtg actgcggtga ctgtcagcgc agcaggttaa caggacgata ctcatcattt
    10321 gccccggtta ttcggggctt ttttgcaggt ggagaacatg atgtttctga aacaggacac
    10381 gtttaattat gaaaaacagt ccgtggtgct cagtgagctg tccgggctgc agagaattga
    10441 atatctgacg tttgttcagc agcgaacggc aaagtttgat gccggggagg gagaactgcc
    10501 ggaggctgaa cgacagattg cttttctgcg gatggggatg gatatcaatg cctggctggt
    10561 ttcccgctca ctgtggaatg cggatcagtc taaggatgta gagacgcttt gcgcatccgt
    10621 tattacaaca tggtcgtatg atgccctggg tgcgggggcg gagatggttc tgtcgctgag
    10681 cggtatgggg gccattgata atgccgtgga tgatgagcat gaggcgctga cgccggaaaa
    10741 gtcctgacgc gggaaatgca gtttgtcatg cggcttgccc gggagttccg gcgggcagac
    10801 tggcggcgga tgctgtcgga aatgtcggcc actgagcttg gtgagtgggg cgattatttc
    10861 cggatgcaga gcttcagtga tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg
    10921 atcgtgagaa tggtgtccgg cagcagtgat gctgcggtgg ctgatttcag ccttttaccg
    10981 gaagagaacg ggataccgga gcgaacggac gaagaactga tgcatcttgg ggaaggtatt
    11041 tccggaggtg tgcgttatgg accagatagc caacctggtc attgatttgg ggattgatgc
    11101 ggcagagttt aaaaatgaaa ttccccgtat caaaaacctt ctgaatggtg cagccagcga
    11161 tgcagaacgg tcttctgccc gtatgcagcg ttttatggag cgtcagactc aggccgcccg
    11221 gcagacaacg caggcagctt cttcggctgc aacagccgca tccgtccatg cgcagacggt
    11281 ggagaagaac gcacaggctc atgaacgcat ggcccgcgag gtggagaaaa cccgccagcg
    11341 catggaggca ctgagccaga aaatgcgcga ggaacaggcg caggccatgg ctctggcgga
    11401 ggctcaggat aaagcggctg ctgcgtttta tcgtcagatt gacagtgtga aacaggccag
    11461 tgcggggctg caggaattac agcgtattca gcagcagatc cgacaggcca gaaacagtgg
    11521 cgggattggt cagcaggatt atctggcgct gatttctgag gttactgcga aaacccgtgt
    11581 tcttacacag gctgaggaag aggctacccg acagaaagtg gcgtttatcc gtcagcttaa
    11641 agagcaggca acccgcctga atctttcatc ttctgagttg cttcgtgcta aggcagccca
    11701 gctgggggta agcagtgctg cagaagtgta tatccgcaaa atggagcagg caggaaaagc
    11761 cacgcattcg ctgggtctga aaagtgcagc agcccgccag gagataggcg ttctgatagg
    11821 tgaactggcc cgcggcaatt taggggcgct gaggggatcc gggataacgc tggctaaccg
    11881 tgccggatgg atagacacac tgatgtcacc gaaaggcatg atgcttggcg gggttattgg
    11941 cggtattgcc gcggccgtct atggtctggg taaagcctgg tatgacggtc agaaggaggg
    12001 ggaagaattt aaccgccagt tgtcgctgac ggggcattat gccggagtca ctgccgggca
    12061 gctgtggacg ctcagtcgtg ctatttccgg gaatggtatc acgcaacatg ctgcagccgg
    12121 tgcgctggct caggtggtgg ggagtggtgc atttcgtgga aacgatatcg gtatggtggc
    12181 gagagttgcc gcacagatgg agcgatcggt tggccagtcg gtcagcgata ccataaatca
    12241 gtttaagcgg ctgaaggatg atcctgtaaa tgccgcgaag gctctggaca atgagctgca
    12301 ttttcttact gccactcagc ttgagcagat acgcgtcctt ggggagcagg ggcggtccag
    12361 tgatgctgca cggatagcca tgtctgcact ggcagaggaa accggtcggc gtactgcgga
    12421 tattgataat aacctcaatg cgcttggcag tacgctgaag tatctgtctg atttgtggag
    12481 tcgtttctgg gatgcggcca tgaatattgg tcgtgaagac tcgctggatg aacagattgc
    12541 cgctttacag gagaaagtgt cgcgggcgaa aagactcccc tggacggcat catcttctca
    12601 ggttgaatac gatcagcagc gtcttaacga tcttcaggag aaaaaacgcc agaaggattt
    12661 gcaggatgca aaagagcagg cagagcggaa ttatcaggag caacagaaac gccgtaatgc
    12721 tgaaaatgct gcactgaacc ggatgaatga aacggaagca gcacgacatc agcgtgaaat
    12781 tgcgcgtatt aatgccatgc agtacgccga tcaggctgtc agggatgcgg cgatacaacg
    12841 tgaaaatgaa cgttacgaga aagccctggc atccggtaag aaaaaaacac gcgaaacccg
    12901 taatgatgag gccacccggt tattgctgca gtacagtcag caacaggcac aggtggaagg
    12961 acagattgct gctgccagac agtcagcagg cattgccacg gaaaggatga cagaagcgca
    13021 taaacagctt ctggctctgc agcagcgcat cagcgacctg gacgggaaaa aactgacggc
    13081 agatgaaaag agtgtgctgg cccgtaaaga tgaactgatt caggcactga cgctgctgga
    13141 tgtaaaacag caggagcttc agaaacagac ggcactcaac gagctgaaga aaaaaacaat
    13201 tcagctgacc agtcaactgg ctgaagaaga gcgcgctcag cgtcagcaac atgacctgga
    13261 tatcgccacg gtgggtatgg gtgatcagca gcgacagcga tatcaggtac aactgagtct
    13321 tcgccagaaa taccagcaac agctggagca gttgaggcgg gatagtgagc agaaagggac
    13381 atataacacg gatgactaca gaaaggccga gcaggcgctg acggagagcc tgaaccgaca
    13441 actgaatgag aatcgccgtt actggcaaca gcttgaagtt gtgcagggta actggaaaaa
    13501 cggagtcctg cgtgcatttc aggattttac cgtggatgca gataatacgg cagaaacagc
    13561 agaacaggtg ttctcgtcag ccttcagcaa catgggaaat ggcctggcaa cttttgtcac
    13621 taccggcaaa ctcaatttca aatccttcac ctcttctgtg ctgtcagata tggcgaaaat
    13681 cctggcgcag gcaaccatga tgaaatcgat aaaagggatt ggcagtgtac tgggatttga
    13741 tctcagcagc ctttccctga atgccaatgg ggggatttat cagtctgctg atttgagtcg
    13801 ttacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag gcgcgggtgt
    13861 gatgggggaa gcgggacctg aagccattct gccattgcgt cgtggtgctg acggtaagct
    13921 gggggttgtg gcggatattg gggggtcagg tatggcgatg ttttccccgc agtacaacat
    13981 cgagatcaat aacgatggca cgaacgggca gataggtccg gctgccctga aggcggttta
    14041 tgacctcggg aaaaaagcgg cagcggactt tatgcaacag caggcccgtg atggtggtcg
    14101 gttaagtgga gcatatcggt aatggagacg tttcactgga aagtgcgccc ggatatgaat
    14161 gtggtatcag agccgaaagt ggtgacagtg aagctgggcg atggttatga acagcgtcgt
    14221 gcggcgggac tgaataacca gttgtcgact tacagcgtga cgatacgtgt tcgtaaatgt
    14281 gaccacccat ctttaaaagc ctttctggaa cggcacggtg gcgtccgtgc atttcagtgg
    14341 acgccacctt atgactggaa accgatcagg gtggtttgtc gtaaatggtc ggcaagcgtg
    14401 ggggcgctgt gggtaaccat aacggcagat tttgaacagg tcgtggcata ggaggctctg
    14461 atgcaggata ttccacagga aacacatcat gagacgacac gcctcactca gtcagcccag
    14521 gtggtgctct gggaaatcga tctgacagag gtcggtggtg aacgttattt tttctgtaat
    14581 gagcagaacg aaaaaggtga gccggttatc tggcaggggc ggcagtatca ggtatacccc
    14641 attcagggga cgggatttga actgaacggc aagggcagtg ctgcccgtcc gacactgacg
    14701 gtttctaacc tgtacggtat ggtcaccggg atggcggaag atctgcagag tctggtcggc
    14761 ggaacggtgg tccggcgtaa ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac
    14821 ggaaacagtg acgccgatcc ggagcaggag gtgatcagcc gctggcgcat tgagcagtgc
    14881 agcgaactga gcgcggtgag tgcctctttt gtactgtcca cgccgacgga aacggacggc
    14941 gctgtttttc cgggacgtat catgctggcc aacacctgca cctggaccta tcgcggtgat
    15001 gagtgcggtt atcacggtcc ggcagtcgcg gatgaatatg accagccgac gtccgatatc
    15061 acgaaggata aatgcagcaa atgcctgagc ggctgtaagt ttcgcaataa cgtcggcaac
    15121 tttggcggct tcctttccat taacaaactt tcgcagtaat cccatgacag agacagaatc
    15181 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgcggct tcgtggtgag
    15241 agcgccggag ggggaaagat attttccctg cgtgaatatt tccggtgagc cggaggatta
    15301 tttccggatg gctccggagg actggctgca ggcagagatg cagggtgaga ttgtggcact
    15361 ggtccacagt catcccggtg gtctgccctg gctgagtgag gctgaccggc ggctgcaggt
    15421 gcagagtgat ttgccgtggt ggctggtctg ccggggggcg attcacaagt tccgctgtgt
    15481 gccgcatctc accgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg
    15541 ggatgcttat catctggcgg ggattgagat gccggatttt catcgcgggg atgactggtg
    15601 gcgtaacggt cagaatctct atctggataa tctggaggcc acagggctgt atcaggtggc
    15661 gttgtcatca gcacaaccgg gcgatgtgct gctgtgctgt tttggttcat cggtgccgaa
    15721 tcatgccgcc atttactgcg gcgacggtga gctgctgcac catattcctg aacaactgag
    15781 taaacgagag aggtataccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
    15841 ggcatggcac gcatctgcct ttacggggat ttacaacgat ttggccgccg catcgacctt
    15901 cgagtgaaaa cggggtctga agccatccgg gcgctggcca tgcagatccc ggcgtttcgt
    15961 cagaaactga gcgacggctg gtaccaggta cgcattgccg ggcgtgatgc aggtgaaacc
    16021 gaattgtctg cccgtcttaa tgagccgctg gcaaatggtg ccgtgatcca catcgtgccg
    16081 cgtctggcgg gagctaaaag tggcggtgtg tttcaggtgg tgctgggggc ggcgctgatt
    16141 gcggtggcat ggtggaaccc tgtgggctgg ctgggtgccg cggctgtatc gggcatgtat
    16201 gcggcagggg ccagtatgat cctgggcgga gtggcgcaga tgctggcacc gaaagccagg
    16261 acgcccacgg cagcaagtac agataacggc aaacagaaca cctatttctc ctcactggat
    16321 aacatggttg cccagggcaa tgttctgccc gttctgtacg gtgaaatgcg cgtggggtcg
    16381 cgggtggtct ctcaggagat cagcacggca gacgaagggg atggtggtca ggttgtagtg
    16441 attggtcgct gatgaaaaac gttttatgtg aaaccgcctc cgggcggttt tgtcgtttat
    16501 ggagcgttag gaatgggtaa aggcagcagt aaggggcata ctccgcgcga agcgaaggac
    16561 aacctgaagt ccacgcagtt gctgagtgtg atcgatgcca tcagcgaagg gccgattgaa
    16621 ggtccggtgg atggattaaa aagcgtgctg ctgaacagta cgccagtgct ggacactgag
    16681 gggaatacca acatctccgg cgtcacggtg gtgttccggg ccggtgagca ggagcagaca
    16741 ccgccggagg gatttgaatc ctccggatcc gagacggtgc tggggacgga agtgaaatac
    16801 gacacgccga tcacccggac catcacgtcg gcaaacatcg accgtctgcg ctttaccttc
    16861 ggtgtgcagg cactggtgga aaccacctca aagggggaca ggaatccatc ggaagtccgc
    16921 ctgctggttc agatacaacg taacggtggc tgggtgacgg aaaaagacat caccattaaa
    16981 ggcaaaacca cctcgcagta cttggcctct gtggtggtgg ataacctgcc gccgcgcccg
    17041 ttcagtatcc ggatgcgcag gatgacgccg gacagcacca cagaccagct gcagaacaaa
    17101 acgctctggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggca
    17161 ctggtcggcg tacaggtgga ttcggagcag ttcggcagcc agcaggtgag ccgtaattat
    17221 catctgcgcg ggcgtattct gcaggtgccg tcgaattata acccgcagac gcgacaatac
    17281 agcggtatct gggacggaac gttaaaaccg gcatacagca acaacatggc ctggtgtctg
    17341 tgggatatgc ttacccatcc gcgctacggc atggggaaac gtcttggtgc ggcggatgtg
    17401 gataaatggg cgctgtatgt catcggccag aattgcgacc agtcagtgcc ggacggcttt
    17461 ggcggcacgg agccgcgcat cacctgtaat gcgtacctga ccacacagcg caaggcgtgg
    17521 gacgttctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg
    17581 ctgacgttcg tgcagaaccg accgtcggat aaggcgtgga cctataaccg cagtaatgtg
    17641 gtgatgccgg atgatggcgc gccgttccgc tacagcttca gcgccctgaa agaccgccat
    17701 aatgccgttg aggtgaactg gattgacccg aacaacggct gggagacggc gacagagctt
    17761 gttgaagata cgcaggccat tgcccgttac ggtcgtaatg ttacgaagat ggatgccttt
    17821 ggctgtacca gccgggggca ggcacaccgc gccgggctgt ggctgattaa aacggagctg
    17881 ctggagacgc agaccgtgga tttcagcgtc ggcgcagaag ggcttcgcca tgtaccgggc
    17941 gatgttattg aaatctgcga tgatgactat gccggtatca gcaccggtgg tcgtgtgctg
    18001 gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aaatcacgct gccatcctcc
    18061 ggtaccacgc tgataagcct ggttgacgga agtggcaatc cggtcagcgt ggaggttcag
    18121 tccgtcaccg acggcgtgaa ggtaaaagtg agccgtgttc ctgagggtgt tgctgaatac
    18181 agcgtatggg ggctgaagct gccgacgctg cgccagcgcc tgttccgctg cgtgagtatc
    18241 cgtgagaacg acgacggcac gtatgccatc accgccgtgc agcatgtgcc ggaaaaagag
    18301 gccatcgtgg ataacggggc gcactttgac ggcgaacaga gtggcacggt gaatggtgtc
    18361 acgccgccag cggtgcagca cctgaccgca gaagtcactg cagacagcgg ggaatatcag
    18421 gtgctggcgc gatgggacac accgaaggtg gtgaagggcg tgagcttcct gctccgtctg
    18481 accgtaacag cggacgacgg cagtgagcgg ctggtcagca cggcccggac gacggaaacc
    18541 acataccgct tcacgcaact ggcgctgggg aactacaggc tgacagtccg ggcggtaaat
    18601 gcgtgggggc agcagggcga tgcggcgtcg gtatcgttcc ggattgccgc accggcagca
    18661 ccgtcgagga ttgagctgac gccgggctat tttcagataa ccgccacgcc gcatcttgcc
    18721 atttatgacc cgacggtaca gtttgagttc tggttctcgg aaaagcggat taccgatatc
    18781 aggcaggttg aaaccacagc ccgctatctt ggcacggcgc tgtactggat agccgccagt
    18841 atcaatatca aaccgggcca tgattattac ttttatatcc gcagtgtgaa cactgttggc
    18901 aaatcggcat ttgtggaggc tgtcggtcgg gcgagcgatg atgcggaagg ttacctggat
    18961 tttttcaaag gccagataac cgaatcccat ctcggcaagg agctgctgga aaaagtcgag
    19021 ctgacggagg ataacgccag caaactggag gagttttcga aagagtggaa agacgccaac
    19081 gataaatgga atgccatgtg gggcgtcaaa attgagcaga ccaaagacgg caaacattat
    19141 gtcgcgggta ttggcctcag catggaggac acggaggaag gcaagctgag ccagtttctg
    19201 gttgccgcta accgtatcgc gtttattgac ccggcaaacg ggaatgaaac gccgatgttt
    19261 gtggcgcagg gcaaccagat attcatgaac gacgtgttcc tgaagcgcct gacggccccc
    19321 accattacca gcggtggcag tcctccggta ttttccctga catcagacgg aaagctgacc
    19381 gctaaaaatg cggatatcag tggcagtgtg aatgcgaact caggaacgct caacaatgtc
    19441 acgattaacc agaactgtac gattaagggc atgctggagg cgacccaggt cagaggggat
    19501 ttcgttaaag ctgtatcaaa agccttcccg aaaaaagtcg gtacgtgggg taacacggaa
    19561 acaccaaacg gtacggttac agtcaccatc agcgatgatc ataactttga ccgccagatt
    19621 attattccgc ccattatttt taacggtata gcgtatgacg atccggggag cggaaataac
    19681 ccaggaggca cgcgatacac gggttatggt tttgaagttc gcaaaaacgg cgtattaatc
    19741 gcatccagag aaactaaagg ggccattccc ggtagttaca gtgcagttat tgatatgcct
    19801 agtggtggtg gtagcgtcac tctggagttt aagattttcc agaaaggcaa tcagggggca
    19861 ggcaatatca ccgattgtac ggtgattgtg accaaaaaag ctgcttccgg catcagtatt
    19921 cgttgaaata tttataaccc caataaaggg cgtcaggaat gacgcctttt ttattgcaga
    19981 aaagcgagag gtaattatgc gtaaagtttg tgcagccatt ttgtccgcag ccatctgtct
    20041 ggccgtatcc ggtgcgcctg catgggcgtc tgagcagcag gccacactga gcgcagggta
    20101 tcttcatgcc cgtacgaacg ctcccggcag cgataatctg aacgggatta acgtgaaata
    20161 ccgttatgag tttacggaca cgctggggct gattacgtca ttcagttatg ccaacgctga
    20221 agatgagcaa aaaacgcatt acagcgatac ccgctggcat gaagattccg tgcgtaaccg
    20281 ctggttcagc gtgatggcgg ggccgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
    20341 gatggcgggt atggcttaca gccgtgtttc gact
//
LOCUS       DAZFJF010000082        20176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFJF010000082 DAZFJF010000000
VERSION     DAZFJF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.082"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..155)
                     /gene="yojO"
                     /locus_tag="ACHKAU_003261"
     CDS             complement(<1..155)
                     /gene="yojO"
                     /locus_tag="ACHKAU_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO5532268.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSL"
     gene            264..758
                     /gene="napF"
                     /locus_tag="ACHKAU_003262"
     CDS             264..758
                     /gene="napF"
                     /locus_tag="ACHKAU_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO5532269.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            748..1011
                     /gene="napD"
                     /locus_tag="ACHKAU_003263"
     CDS             748..1011
                     /gene="napD"
                     /locus_tag="ACHKAU_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO5532270.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1008..3494
                     /gene="napA"
                     /locus_tag="ACHKAU_003264"
     CDS             1008..3494
                     /gene="napA"
                     /locus_tag="ACHKAU_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFO5532271.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3501..4196
                     /gene="napG"
                     /locus_tag="ACHKAU_003265"
     CDS             3501..4196
                     /gene="napG"
                     /locus_tag="ACHKAU_003265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO5532272.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4183..5046
                     /gene="napH"
                     /locus_tag="ACHKAU_003266"
     CDS             4183..5046
                     /gene="napH"
                     /locus_tag="ACHKAU_003266"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO5532273.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5043..5492
                     /gene="napB"
                     /locus_tag="ACHKAU_003267"
     CDS             5043..5492
                     /gene="napB"
                     /locus_tag="ACHKAU_003267"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO5532274.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5502..6104
                     /gene="napC"
                     /locus_tag="ACHKAU_003268"
     CDS             5502..6104
                     /gene="napC"
                     /locus_tag="ACHKAU_003268"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO5532275.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6117..6740
                     /gene="ccmA"
                     /locus_tag="ACHKAU_003269"
     CDS             6117..6740
                     /gene="ccmA"
                     /locus_tag="ACHKAU_003269"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO5532276.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6737..7399
                     /gene="ccmB"
                     /locus_tag="ACHKAU_003270"
     CDS             6737..7399
                     /gene="ccmB"
                     /locus_tag="ACHKAU_003270"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO5532277.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7441..8178
                     /gene="ccmC"
                     /locus_tag="ACHKAU_003271"
     CDS             7441..8178
                     /gene="ccmC"
                     /locus_tag="ACHKAU_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO5532278.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8175..8384
                     /gene="ccmD"
                     /locus_tag="ACHKAU_003272"
     CDS             8175..8384
                     /gene="ccmD"
                     /locus_tag="ACHKAU_003272"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO5532279.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8381..8860
                     /gene="ccmE"
                     /locus_tag="ACHKAU_003273"
     CDS             8381..8860
                     /gene="ccmE"
                     /locus_tag="ACHKAU_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO5532280.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8857..10800
                     /gene="ccmF"
                     /locus_tag="ACHKAU_003274"
     CDS             8857..10800
                     /gene="ccmF"
                     /locus_tag="ACHKAU_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO5532281.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10797..11354
                     /gene="dsbE"
                     /locus_tag="ACHKAU_003275"
     CDS             10797..11354
                     /gene="dsbE"
                     /locus_tag="ACHKAU_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO5532282.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11351..12403
                     /gene="ccmH"
                     /locus_tag="ACHKAU_003276"
     CDS             11351..12403
                     /gene="ccmH"
                     /locus_tag="ACHKAU_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO5532283.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(12438..13085)
                     /gene="narP"
                     /locus_tag="ACHKAU_003277"
     CDS             complement(12438..13085)
                     /gene="narP"
                     /locus_tag="ACHKAU_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO5532284.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13405..15996
                     /gene="yejO"
                     /locus_tag="ACHKAU_003278"
                     /pseudo
     CDS             13405..15996
                     /gene="yejO"
                     /locus_tag="ACHKAU_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16464..17387)
                     /locus_tag="ACHKAU_003279"
     CDS             complement(16464..17387)
                     /locus_tag="ACHKAU_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5532285.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            complement(17377..17538)
                     /locus_tag="ACHKAU_003280"
     CDS             complement(17377..17538)
                     /locus_tag="ACHKAU_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532286.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            complement(17550..18038)
                     /locus_tag="ACHKAU_003281"
     CDS             complement(17550..18038)
                     /locus_tag="ACHKAU_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5532287.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            complement(18166..18327)
                     /locus_tag="ACHKAU_003282"
     CDS             complement(18166..18327)
                     /locus_tag="ACHKAU_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO5532288.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            complement(18331..18525)
                     /locus_tag="ACHKAU_003283"
     CDS             complement(18331..18525)
                     /locus_tag="ACHKAU_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532289.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            complement(18656..18901)
                     /locus_tag="ACHKAU_003284"
     CDS             complement(18656..18901)
                     /locus_tag="ACHKAU_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532290.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            complement(19103..>20176)
                     /locus_tag="ACHKAU_003285"
     CDS             complement(19103..>20176)
                     /locus_tag="ACHKAU_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO5532291.1"
                     /translation="KRENWREYLERERGKKPGEEKHHQRKTTLPMSVGSAGYDTQLDY
                     VVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGI
                     GVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIAARQVERETGKPVRMIIL
                     DTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRA
                     SLDAEYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVV
                     DKPRPPVELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHF
                     RRWLYKLENDGVIVIDGDDVRPL"
BASE COUNT         4462 a         5024 c         5560 g         5130 t
ORIGIN      
        1 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
       61 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
      121 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
      181 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
      241 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
      301 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
      361 ttctgaccca ttgcacccgc tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
      421 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
      481 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
      541 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
      601 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
      661 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
      721 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
      781 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
      841 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
      901 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
      961 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     1021 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     1081 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     1141 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
     1201 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
     1261 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
     1321 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
     1381 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa aggaccggaa tcgatcggta
     1441 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgttcaaag
     1501 cgggcttccg ttcgaacaac atcgacccga acgcacgtca ctgtatggcg tcggcagtag
     1561 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
     1621 aggctgacgc gtttgtgctg tggggcgcta acatggcgga gatgcacccg atcctctggt
     1681 cacgcatcac taaccgtcgt ctctctaacc agaacgtcac cgtggcggtg ctttctacct
     1741 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
     1801 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
     1861 tcttcagcaa gcacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
     1921 cgacccatcc gctggaaaaa gcagcgaaga atccgggttc tgacgcctcc gaaccgatga
     1981 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaact gccgaaatga
     2041 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
     2101 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggccaaca
     2161 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
     2221 tctccctgac cgggcagcct tccgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
     2281 accgtctgcc tgcggacatg gtggtgacta acgagaaaca ccgtgatatt tgcgagaaga
     2341 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
     2401 accgtgcact gaaagacggc aagctgaatg tttactggac catgtgtacc aacaacatgc
     2461 aggccgggcc gaacattaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
     2521 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc agccgacttg atcctgccga
     2581 ccgcaatgtg ggtagagaaa gagggcgctt acggtaacgc cgaacgccgt actcagttct
     2641 ggcgtcagca ggtacaggcg ccaggcgaag cgaaatcgga tctctggcag ttagtccagt
     2701 tctcccgccg cttcaaaact gaagaagtat ggccggaaga gctgctggcg aagaaaccgg
     2761 aactgcgtgg caaaacgctg tacgaagttc tgtatgccac ccccgaagtg agcaaatacc
     2821 ctgtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
     2881 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctgg
     2941 caccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
     3001 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
     3061 agttctacgg taaaccggat ggcaaagcgg tgatcttcgc actgccgttc gaaccggcgg
     3121 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
     3181 ggcacaccgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
     3241 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
     3301 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
     3361 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactga
     3421 cgctggatgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
     3481 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
     3541 ttctgcgcga tgtcgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
     3601 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
     3661 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
     3721 acaccttaaa actggcgacg ctggcctctg gtctgtcggc gggcacgcca tattttgtcg
     3781 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccaagcg
     3841 gtgcgctgga tcgtgagatt gaatcgatcg acgacgcgcg gatggggctg gcggtactgg
     3901 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
     3961 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
     4021 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggttgcggt aagtgcgaaa
     4081 aagtatgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
     4141 agttaggtca ccattaccgc ttcggttggc tggaggggaa caatggcaaa tcgtaaacgt
     4201 gacgccgggc gcgaggcgct ggagaaaaaa ggctggtggc gcagtcaccg ttggctggtg
     4261 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc atggtttggt
     4321 gtgtggatct tgcacggcaa ctacagcagt agcctgttat tcgacaccgt tccgctgacc
     4381 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
     4441 accggtgcgg tgattatcac cgtgctctac gccctggcgg gaaagcgatt attttgcagc
     4501 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
     4561 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
     4621 ctgggatcgg ccttaactgg cacgctcatc tgggaatgga tcaaccccgt atctctgatg
     4681 gggcgcagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
     4741 tttgatctac tggtcgttga acacggctgg tgcgggtaca tttgtccggt tggcgcgctg
     4801 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccaccgatcg gcagaaatgt
     4861 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
     4921 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
     4981 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
     5041 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
     5101 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
     5161 ccgggacaca ggaaggggcc attcggatgc caaaagagca ggaacggatg ccgctgaact
     5221 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
     5281 ccaaccgctg cttgcagtgc cacggtgtcg aaagttatcg caccactggc gcgccgcgta
     5341 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
     5401 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
     5461 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
     5521 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
     5581 ggacgctgct gttgatcggt tttgttggcg gcatcgtctt ctggggtggc tttaacaccg
     5641 ggatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgcaacacgg
     5701 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
     5761 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
     5821 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
     5881 atcgtctgac gatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
     5941 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
     6001 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
     6061 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
     6121 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
     6181 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
     6241 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
     6301 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
     6361 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
     6421 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
     6481 aagatattcc tgtaaatcag ctctcggccg ggcaacaacg ccgcgtcgct ttagcgcgtc
     6541 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
     6601 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
     6661 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
     6721 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
     6781 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
     6841 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
     6901 ggttgctgca ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
     6961 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
     7021 gaaggtgatg gcgcactgga tggtaagcgg tctgccgtta ctcattcttt cgccactggt
     7081 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
     7141 gggaacgcct acgcttggct ttctcggtgc accgggcgtg gcgctgacag tgggacttaa
     7201 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
     7261 cgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
     7321 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
     7381 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
     7441 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
     7501 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
     7561 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
     7621 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
     7681 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
     7741 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
     7801 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctttt tttgtatgtg
     7861 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
     7921 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
     7981 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
     8041 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
     8101 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
     8161 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
     8221 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
     8281 tcgtgcactc ggtgatgcag catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
     8341 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
     8401 ccgcttgtgg attgcctgtg ctgttctggc agggttggca ctgactatcg gtctggtgct
     8461 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
     8521 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtctg cgcgttggcg ggatggtgat
     8581 gccgggtagc gtgcagcgcg atcccaattc gctaaaagta actttcacca tttacgacgc
     8641 tgaaggttca gtagatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
     8701 gggcgttgtg gtgcagggcg aactggaaaa aggcaatcat atcctcgcaa aagaagtgct
     8761 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagccaatca
     8821 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggtaacggg
     8881 ctgctgtgtc tggcgctagg aattgcgctg ctgctgtccg tgtatccgct atggggcgta
     8941 gcgcgcggag atgcgcgcat gatggcgtct tcccgcttgt ttgcctggct gctgtttatg
     9001 tctgtggctg gcgcatttct ggtactggtc aatgccttcg tggtcaacga cttcaccgtc
     9061 acctatgttg ccagcaactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
     9121 tggggcgcgc atgaaggctc gctcctgctg tgggtgctgc tgatgagcgg ctggaccttt
     9181 gcggtagcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
     9241 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccgttctca
     9301 cgcacgttgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggatccg
     9361 gggctgatct tccatccgcc tctgctctat atggggtacg tggggttctc ggtggcgttt
     9421 gcttttgcca ttgcttcttt gttgtgcggg cgtctggaca gcacttatgc gcgttttact
     9481 cgtccgtgga cgctggcggc gtggatcttc ttgacgctcg gcattgtgct cggttccgca
     9541 tgggcctatt acgaactcgg ctggggcggc tggtggttct gggatccggt agaaaacgcc
     9601 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
     9661 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
     9721 ctggggactt tcctggtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcctctgat
     9781 ccggcacgcg gtatgtttat cctcgccttt atggtactgg tgattggtgg ttcgctgctg
     9841 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
     9901 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtattactg
     9961 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    10021 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctgct gcttggtgtc
    10081 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    10141 gccttcatct caacgctggt gctgtcgctg ctgctgccgt ggctgttcga aagcaaagtt
    10201 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgctgtgct ggcaattgcg
    10261 gaagctgcgc tgcgtatttc acgtggcacg aaaaccacct tcagttattg gggaatggtg
    10321 gcggctcacc tggggctggc agtgacaatt gttggtattg cctttagcca gaactatagc
    10381 gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg atattcatga atatcgcttc
    10441 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    10501 ggcgtaacgc gcgatggcaa gccggaaacg gtgctgtatg cggaaaaacg ttattacaac
    10561 actgccgggt cgatgatgac cgaagcggca attgacggcg gcatcacgcg tgacctgtac
    10621 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggccg tgcgtcttta ctacaaacca
    10681 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact actgtgtctg
    10741 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggccgtatga
    10801 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    10861 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    10921 gcaagcctgt gccgaagttt cgtctcgaat cactggacaa tccggggcag ttttatcagg
    10981 cggatgtgct gactcagggc aaaccagtac tgcttaacgt ctgggcgacc tggtgtccga
    11041 cctgccgtgc ggaacatcaa tatctgaatc agctttcagc gcagggcatt cgcgtggtcg
    11101 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    11161 cttacgcgct aagcctgttt gatggcgacg gcatgttagg gctggatctc ggtgtctatg
    11221 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    11281 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    11341 aggccgcaca atgaggtttt tattgggcgt gctgatgctg atgatctccg gctcagcgct
    11401 ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    11461 cactgaagaa ctgcgctgcc cgaaatgcca gaataacagc attgccgatt ccaactcgat
    11521 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaagaa
    11581 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    11641 aacgccgctg accgtgctgc tgtgggtgct gccagtagtg gctattggca ttggcggttg
    11701 ggtcatttac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    11761 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    11821 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    11881 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagccga
    11941 tccgctcaac gaagaagaga tgtcgcgtct tgcgctgggg atgcgtactc aactgcaaaa
    12001 aaatccggga gatatagaag gctggattat gttgggccgc gttggcatgg cgctgggtaa
    12061 cgctagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagcga
    12121 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    12181 cggcggcgaa ctgctgcgcc agttggtgag aagcgaccac agcaatatcc gtgtgttaag
    12241 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    12301 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    12361 cgcgcaggcg atgcaacatt tgtcgccgca ggagaataaa taaaaagagg cccgatggtg
    12421 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggctgcc
    12481 acgcgtgagc ggacattgag tttgcgcagc agattgcgaa tatgtacttt tactgtctgc
    12541 tctgaaatat tcaacaccga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    12601 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    12661 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctcca
    12721 gcacgaattg cttccagcaa tacttccggg tcgctgtctt tcaacagata accgtctgcg
    12781 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatctg atacggtcag gataataatt
    12841 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    12901 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    12961 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    13021 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    13081 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    13141 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    13201 cttgtcgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    13261 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    13321 aaaactgact cccggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    13381 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    13441 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    13501 gagtatggtg agacagttga tggtgttgtc ctggaaaaag atatccagct ggtttatggg
    13561 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    13621 agtaataata ctgaaattaa cggagggtat cagtacattg aaatgaatgg cgccgcagaa
    13681 tactcagtat taaatgacgg ttatcaaatt gttcaaatgg gtggcgcggc aaaccagact
    13741 acgctcaata atggtgtgct acaggtttat ggcgcagcga atgataccac gattaaaggc
    13801 gggcgcttaa tcgttgaaaa agatgggggg gccgtctttg tcgctatcga aaagggagga
    13861 ctactggagg ttaaagaggg gggattagcg attgcggtag atcagaaagc aggcggtgct
    13921 attaaaacaa ccacgcgggc catggaggta ttcggaacaa accgtctcgg tcagttcgat
    13981 atcaagaatg gtattgctaa taatatgttg ttggaaaacg gcggaagttt gcgagttgaa
    14041 gaaaatgact tcgcttataa taccactgta gatagtggcg gcttactgga ggttatggat
    14101 ggcgggactg taactggcgt tgataaaaaa gcaggcggaa aattaattgt ctcaacgaat
    14161 gcgctggaag tgagtggtcc aaacagtaaa ggccaattta gtataaaaga tggtgtgtca
    14221 aaaaattatg aactggatga tggttccggg ctcattgtta tggaggacac gcaggccatt
    14281 gatactatcc ttgatgagca tgccactatg caatcgctgg gaaaggatac tggtacgaaa
    14341 gtgcaggcaa atgcggtata tgatctcggt cgatcatatc agaatggaag tatcacgtat
    14401 tcctcaaaag ccatctctga aaatatggtt atcaacaatg gccgcgctaa cgtctgggct
    14461 ggcacaatgg ttaacgtttc agtcagaggg aatgatggca ttcttgaggt catgaagccg
    14521 caaataaatt atgcacccgc aatgttggtg ggtaaggtag tggtttctga gggcgcttct
    14581 tttagaacgc atagtgccgt ggataccagc aaagcggacg tttcgctcga aaatagcgta
    14641 tggaccatca ttgccgatat cactacgacg aaccaaaaca ccctcctcaa cttagccaac
    14701 cttgcgatgt ctgacgcaaa tgtgattatg atggatgagc cagtgactcg ttcatcagtg
    14761 acggcaagtg cggaaaattt cattacgttg accaccaata ccctgtcggg aaacggcaat
    14821 ttttatatgc gtaccgatat ggctaatcat cagagcgatc agctcaacgt caccggtcag
    14881 gcaacaggtg atttcaaaat attcgtgacg gacaccggtg ccagcccggc agcaggagat
    14941 agccttacac tggtaacaac gggcggcggt gatgctgcat ttacgttggg caatgccgga
    15001 ggcgttgttg atatcggtac gtatgaatat accttgctgg ataatggcaa ccatagctgg
    15061 agtttggcag agaatcgcgc gcaaattacc ccttcaacca ctgatgtgct gaatatggcg
    15121 gcagcacaac cgctggtatt tgatgcagaa ctggacaccg tgcgtgagcg tcttggtagc
    15181 gtaaaaggcg ttaattacga tacggcgatg tggagttcgg caattaacac ccgcaacaac
    15241 gtgaccactg atgcgggcgc tggctttgag caaacattga ccgggctgac gctcggtatc
    15301 gatagccgtt tctcccgtga agaaagcagc acaattcgcg gcttgttctt tggttactct
    15361 cattctgata ttggttttga tcgcggcggc aaaggcaatg tcgatagcta taccctgggg
    15421 gcttatgccg gttgggagca tcagaacggt gcctatgttg atggagtggt gaaagttgac
    15481 cgttttgcca acaccatcca tggcaagatg agtaatgggg caacagcgtt tggcgattac
    15541 aatagtaacg gcgcgggtgc tcatgtcgag agcgggttcc gttgggttga cggattgtgg
    15601 agtgttagac cctatctggc ctttaccggc tttaccacag atggtcagga ctacacgtta
    15661 tcaaacggca tgcgcgcgga tgtgggaaat acccggatat tacgcgctga agcgggaacg
    15721 gcggtaagct atcacatgga cctgcaaaac ggtacgacgc tggaaccctg actgaaagcc
    15781 gccgtgcgtc aggaatacgc cgattctaac caggtgaaag ttaatgacga tggcaaattt
    15841 aataatgatg tggctggaac ccgtggcgtt tatcaggctg ggataaggtc atcgtttacc
    15901 ccgacgttaa gcggtcattt gtcagtcagc tatggcaatg gcgcaggggt agaatcgccg
    15961 tggaatactc aggcgggtgt ggtctggacg ttctgataac aaaaaataaa caggctgtga
    16021 tgtgtcacgg tctgtttatc gaattaattg cagatataaa aaaaaccaac cgtaaaggtt
    16081 ggttttttct tgggattttt ggcacgagag tagtttaatt gagtatgtaa ctacatgatt
    16141 ttaaatagta tgcttgattc aacttttaaa ccgtataact aaacgtataa ctaaaatcat
    16201 ttgctgaaaa agtgtacaat ttaaatgcga gttttcctgt ggtaaaagct ccaaccgtaa
    16261 cgatgttcgt tatggttgcc ttgatggtct gcgtggtcat tatgaccatt caggatttga
    16321 agggtgacgc ttccgacttc ctcaaattga ggtttccgaa aatatcagcg ggttagcgat
    16381 taatctgacc attaattaaa ctgcgaaaat ttcgtaatta tcccgatatg gggatcacca
    16441 tatcgggatt gatgccggaa aaatcagctt ttcgccagaa cggtgacggc cttatcgagt
    16501 ttttgccatc cgcaataggt gattgccctg aacgctgtag cgtcattctg gaaaaggcta
    16561 accgtccctg attcggtttt tattaccgcc tcatcagttg gggttataac cgtggcttca
    16621 acgttaataa tcagcttttg agcgtctatc agcatcatgc gatccgcctg ttcgctcaca
    16681 attaccggta ttctcaaaag ctctatctgc cagaactcag catcagtaag ctgtaaggcg
    16741 ttttctacgg gcaagattag cgacgacgtg gcaagcgtgt taccgttcgc tatgtgcttt
    16801 ttgaaaagct ctatgaagtt ttcggcctca tcagcaccat taagcacgcc ctcaggggat
    16861 acgcccgcca cctcctggtc actggaaaga aattttttat ctatttcttt caccgattcc
    16921 gatgtgatca agtctctgat cgctgaattg gtaccagggg tgctaaattt gattagctcg
    16981 ttattcagca tcacaatagc gccaagtttg cacagtttta gcgcactttc gtttacttct
    17041 ccctctttga cagggatttc ttcggcttct ttaagccatt tactaccctt gcctgatatt
    17101 gactgcaatt ttgcgccaaa cggaagattc agcgcgctgc tgattagctt gcctggtaca
    17161 gactgcgaaa aaatatactc gccaagctct cttactatcg gctctgccac cagcgacggc
    17221 atattatccg tagtcattcc ggcaacaatg gatttttcaa gaaatttcga tactgccgga
    17281 tcattttttt tcgtcatttc aattgcgtct gcatggctga atttacacga tgtaagcacg
    17341 ctggcggcgc gaactgccga cacgcctttc atgtttttag aaatcattat cttcttcctc
    17401 ctggtaacat ggggttaggg tttcgtgaaa atatggcatc agaacagcgg cagcctgtaa
    17461 ccgtatttcg gttggctgct gcttgtcgtt catgatttcc gtgagtacat caatagggtc
    17521 tgtgatgtat tcgctcatag ttcgctacct cagtgtttta agccagttgg tggcttaggg
    17581 atggggaacc gatttgagga gacattagcg gcattgagtt ttttaacctc ttttttccca
    17641 atgctgccga tcttgcggtg ggtgaactgg gccagcttaa acgcggcatc taaagccagt
    17701 tttggatctg tgtccatgtt gtcaatcagt atcctgccca ttgctttgat ggggtcgggt
    17761 aggccgttat ccatcatctc aatggtgtta attgatgccc gttccggtgt aactttttcg
    17821 gtaactttct ttgtaacttt tttcggtttt gtaacctgaa aagttacatc agaagttaca
    17881 gccttaagat actttttcac ctccggatca ttggatagct gccagccttt aacccttgca
    17941 gtcttttcgc tgtatcccgc atgtatcgcg gcttccgttg gatttgtgcc ctttgataac
    18001 gcatcagcaa accgcttttt tttggtcgtt aatgccatgc aaaaacctcg ttttgagggg
    18061 gtgtaacttt tcgtgtaact tttttgaaaa gcaaaatttt ctatgaatga gactgggggc
    18121 ggtgtccagg gcgatcgact ttccggccgg aacactcccc ccctgtcatt gctcaccgag
    18181 taacgagatc aggccttcaa cttcatcact cagcttgttt attcgtgtct caagctctgc
    18241 gatcttttcc atcaatggcg ctatggtgtc tcttgtttcc tcgccaatgg ctttaatgat
    18301 gcttgcctcg cgttctgtga tagccattgt ttacgcctca cttgttgcaa tgcccaggcg
    18361 ttcgcgtaac agtacgcgcg caagatgccc tattgttggt gttgccgccc tgccgtagat
    18421 acctgattca ttcatccggc gcacaatatc gaactgcaac tttttaagct cgttaaggat
    18481 ttcatgatca atcatcacgg tagcgctttg ctttatctct tccactttgt tttctcctga
    18541 tacttatcat tgcataacca gtattttcga tagattatca ttaattaacc atctttgaag
    18601 atatatgaga tcgaaaaaaa gccggattgc tccggctatg tgctcacttc ttcggttatt
    18661 ctccagcgct tttaacgttg atggttgtta cctgttccgc ttcggcaatc tcccgttctg
    18721 tcagcgtggc aaagtttgcc gccgctgtgg tcatgaatgc gcttatcagt tcgggatgct
    18781 ccttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc tttaaggaaa
    18841 gctctgtcat gtctaacgct ttatattttg gcgctgtctt atctctggtt tttctgttca
    18901 ttccccacta ctccccactt ttgctcccca ctttttgaaa ttacccaccg tctccccacc
    18961 tctttttttt gtgattctaa cgttatgttt ttcaatatgt tttttactcc ccactttttt
    19021 gggtatatac aggtgggaaa gtgggtaatt atgtttcaat tttgttaaat tccccactcc
    19081 ccccactttt actccccact ttttacagcg ggcgcacgtc atcaccgtca ataacgatta
    19141 cgccatcatt ttccagcttg tacagccagc gccggaagtg cttcatctca taccccagct
    19201 ttttcatatc gtcgcggaga agggcgatag tgcacgcctc cccgtgtgct gttcgtgttc
    19261 tgatgcactg ccatagcgcg gcgtgattct ccgtcttgtt ccctgcctcc tcgatgcgct
    19321 ccagttcaac gggagggcgc ggcttatcca ccaccaccag cgacgtgatt aactccccgt
    19381 cagcgtcggt aaaaagctcc accacgcgta agtcatatgc tgcttctttg agttcctccg
    19441 cgtccttcat tttggtacat gagataacca gcgcttcgct gcctgcgtcc tccctgcgta
    19501 tccggtattc ggcgtccagt gatgcacgaa atgcactgga accacgcgcc ccctttgttt
    19561 catccttgcc ggaatggtga accaccagca ccgttgcccc tgtgcgccgt ttcagttcgt
    19621 cacacccacg gataaacgcc cccatatcac gggaatcatt ttcatcattg ccgccaaagc
    19681 agcgcgccag cgtatccaga ataatcattc gaaccggttt acccgtttcc cgttccacct
    19741 gacgggcagc gataaccatt tcctcaacat caagcggggc agccggaaag atgggacggt
    19801 ttaccagata cagatttttc acctgctcac cgtgcacaac ctcccaggct tttacacgac
    19861 gcggaacgcc gataccgcct tcaccaacca catagagaac agcgccatgc gccaccctgc
    19921 ggcctcccca ctggcggccc gtggaaacgt gacacgccca cgaacccgca aggaacgatt
    19981 tatatgaacc gctcgccccg tatatgctgc acagcgatac cgccggaata atccccttaa
    20041 ccacgtaatc aagttgtgtg tcgtatccgg cagatcccac gctcattggt agcgtggttt
    20101 ttcgctggtg gtgtttttct tcccctggct ttttcccccg ctcccgttcc agatattcgc
    20161 gccagttctc ccgctt
//
LOCUS       DAZFJF010000083        20151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFJF010000083 DAZFJF010000000
VERSION     DAZFJF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.083"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..704
                     /locus_tag="ACHKAU_003286"
     CDS             54..704
                     /locus_tag="ACHKAU_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5532292.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            875..1110
                     /locus_tag="ACHKAU_003287"
                     /pseudo
     CDS             875..1110
                     /locus_tag="ACHKAU_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1520..1621)
                     /locus_tag="ACHKAU_003288"
                     /pseudo
     CDS             complement(1520..1621)
                     /locus_tag="ACHKAU_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1845..1970
                     /locus_tag="ACHKAU_003289"
                     /pseudo
     CDS             1845..1970
                     /locus_tag="ACHKAU_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(2030..2536)
                     /gene="yciE"
                     /locus_tag="ACHKAU_003290"
     CDS             complement(2030..2536)
                     /gene="yciE"
                     /locus_tag="ACHKAU_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5532293.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(2582..3082)
                     /gene="yciF"
                     /locus_tag="ACHKAU_003291"
     CDS             complement(2582..3082)
                     /gene="yciF"
                     /locus_tag="ACHKAU_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5532294.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(3168..3347)
                     /gene="yciG"
                     /locus_tag="ACHKAU_003292"
     CDS             complement(3168..3347)
                     /gene="yciG"
                     /locus_tag="ACHKAU_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5532295.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(3728..4534)
                     /gene="trpA"
                     /locus_tag="ACHKAU_003293"
     CDS             complement(3728..4534)
                     /gene="trpA"
                     /locus_tag="ACHKAU_003293"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO5532296.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(4534..5727)
                     /gene="trpB"
                     /locus_tag="ACHKAU_003294"
     CDS             complement(4534..5727)
                     /gene="trpB"
                     /locus_tag="ACHKAU_003294"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO5532297.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(5739..7100)
                     /gene="trpCF"
                     /locus_tag="ACHKAU_003295"
     CDS             complement(5739..7100)
                     /gene="trpCF"
                     /locus_tag="ACHKAU_003295"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO5532298.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(7101..8696)
                     /gene="trpD"
                     /locus_tag="ACHKAU_003296"
     CDS             complement(7101..8696)
                     /gene="trpD"
                     /locus_tag="ACHKAU_003296"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO5532299.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(8696..10258)
                     /gene="trpE"
                     /locus_tag="ACHKAU_003297"
     CDS             complement(8696..10258)
                     /gene="trpE"
                     /locus_tag="ACHKAU_003297"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO5532300.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(10280..10373)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(10350..10394)
                     /gene="trpL"
                     /locus_tag="ACHKAU_003298"
     CDS             complement(10350..10394)
                     /gene="trpL"
                     /locus_tag="ACHKAU_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO5532301.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            10532..11413
                     /gene="rnm"
                     /locus_tag="ACHKAU_003299"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             10532..11413
                     /gene="rnm"
                     /locus_tag="ACHKAU_003299"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO5532302.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11410..12030
                     /gene="yciO"
                     /locus_tag="ACHKAU_003300"
     CDS             11410..12030
                     /gene="yciO"
                     /locus_tag="ACHKAU_003300"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO5532303.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            12131..13003
                     /gene="rluB"
                     /locus_tag="ACHKAU_003301"
     CDS             12131..13003
                     /gene="rluB"
                     /locus_tag="ACHKAU_003301"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO5532304.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(13043..13633)
                     /gene="cobO"
                     /locus_tag="ACHKAU_003302"
     CDS             complement(13043..13633)
                     /gene="cobO"
                     /locus_tag="ACHKAU_003302"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5532305.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(13630..14388)
                     /gene="yciK"
                     /locus_tag="ACHKAU_003303"
     CDS             complement(13630..14388)
                     /gene="yciK"
                     /locus_tag="ACHKAU_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO5532306.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            14608..15657
                     /gene="sohB"
                     /locus_tag="ACHKAU_003304"
     CDS             14608..15657
                     /gene="sohB"
                     /locus_tag="ACHKAU_003304"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO5532307.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(15693..15944)
                     /gene="yciN"
                     /locus_tag="ACHKAU_003305"
     CDS             complement(15693..15944)
                     /gene="yciN"
                     /locus_tag="ACHKAU_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO5532308.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            16324..18921
                     /gene="topA"
                     /locus_tag="ACHKAU_003306"
     CDS             16324..18921
                     /gene="topA"
                     /locus_tag="ACHKAU_003306"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO5532309.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            19131..20105
                     /gene="cysB"
                     /locus_tag="ACHKAU_003307"
     CDS             19131..20105
                     /gene="cysB"
                     /locus_tag="ACHKAU_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO5532310.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         5049 a         4862 c         5166 g         5074 t
ORIGIN      
        1 atggcttttt gttaattatc gaaaagtaag atatatgctt tagggataaa attatgccat
       61 tttcatttaa tttgtcttct ggaaattact tgtcaacaca agatgttgaa gttttacaac
      121 gtgcgaccag agatcatcaa atggaaagac tcacaatagg agaaagatcg ttttctgttc
      181 gctatcagag tgctatggac gcttttattg tagatcctgt tcaaggggaa ctatattcgg
      241 gtttaagcca tacagaacta gccgatatca ttagattggc tgattctgtt gaaaatcaat
      301 tgaatggagg caattcattt cttgatgtat tcagtacata tatggggcag gttatttctg
      361 aatttatgca tagtaatgat aacagaattg aattgttaca gcggcgatta cattcatgtt
      421 catttttagt taatattgaa gaaatgtctt acatagatga agcattacag tgcccgatta
      481 cgctggcaat tcctcaacga ggtgtttttt taagaaatgc tgaaggttcc agagtatgta
      541 gtttatatga tgaaatggct ctttctcgta taattaatga tgggatgcat cacccactaa
      601 gcagagagcc aataacatta tcaatgcttg tggccagaga gcagtgtgag tttgattgca
      661 gtatcggtca ctttacggtg aggagtgatt gttattcagt gtagactcgg tacacgattt
      721 tgtgtgaaga gactcttatt aaaagttagc atcaaagcgg tcatcaaact tgataataaa
      781 gtgactcatt gctattcgct ggtccttaag ggatattgcc cattttgtat cgtgaactga
      841 atagcaagta gccaccgtcc tgatttcaac gcattatcag gcgcttctga agcggcatac
      901 ctgcatgaca ggatgagtgc aaaggggcgc tgttacgata atgctagggc ggaaagtttc
      961 ttccactcac taaaagtcga atgcatttac ggctaatatt ttatgagtcg ggaaataatg
     1021 cagacggctg tgtttaatta gatctagtgt gattacaatc ggtggtgccg acacagtact
     1081 tgtggctgtc tcagcacgga actgttttga aaaacagaac cttgattagg gctgtgtcca
     1141 cattacgtag gtaggatcac cccacaggca ccacatgtca tgcatggatt aggattgaat
     1201 gataaatcgt aaagaaaact gcaacacctc gcgtgtagca cctggttagc gaactttcga
     1261 aagtacgagt attgtatgat ttccagcagt tatcaatttt acgtattaat cagtgaatca
     1321 acaactcgtc agcagactct ctttgctgtt gaattttttg gaacgtgtat agggcagaaa
     1381 tttttgtttt gggacatggt tgggacgccg tagtacatac gaactctgcc gaataacgta
     1441 tgatattatt ttttaatctc ttggaataca ttatagaaac agaatgctct tgggcgattt
     1501 ttagtgattg tttaaatagc cctgtcatgc agttaaagtg gcgggcatcc tcttcaagga
     1561 tggtgatcct caaccgcacc catttaggat gcgactattg tggtagaaca atgtaaacca
     1621 taattaatct gcgtgcggat acatgcttca tagataggat attctggttt gggttgtgaa
     1681 tgtgttggtc atcgagtgga caataaggta agaggagtaa ctcctgcaaa acaaaaaaag
     1741 agttcaacaa aatcaatcag ttaaaaacac gagagatctg tataaaaaag catcgtaaat
     1801 tctgtatttt tcctttaaat ataatatgtt attgagtttt gtaaaatcta ccagtgtggt
     1861 acatggatat cgataccacc gccaattata agctaggcgg tgcacagcaa cacgatagcg
     1921 tacgcctcga tccgtgggtg tttatgttct cagcaggata tcgtttttaa ttccgcacaa
     1981 aaacgacccc gtaatatacg gggtcaataa ggacatggta taaagggtat tatttcttcg
     2041 cttctacgcc atcagtttca gaacgaatta aaaatttctc agttgtttgc ggaatatgct
     2101 gaatcagcca gtcggccatt tgcttttcct catttaaaat cgcttcgatg atcggaattg
     2161 agactgtatc accggcattt tttgctgctg ctaatagtga ggtgtaacag gcgatttcaa
     2221 attgctcgaa gacatatccg ctaatagagc ctttaactat ttcatcagaa gggaatatac
     2281 caccgattga ctgcccaagc gcagccattt tactcatgga atctttaatg actgaacgtg
     2341 aaatgtcatt acgatcaaga atagtttcca gttgaacaat ctggtttttg gtttcactaa
     2401 gatgttgttc aatacgagcg cgtagttcag gataattatc tatacggctg gccatggatt
     2461 caagcataga ttcggcttgc ttttccattg cgtgggcgtc acgtaaccag tcatgataat
     2521 gttcaatacg attcatgaaa atgtcctctt tataaaatga ctaaaagtta aaattcatat
     2581 ttcaggcttt attttcggct ttcttattta cgttattaag ggccagatca gtcagtttga
     2641 tgtcggtggc cttttcttct tccagggttt ctttcagaag cttcgctgct ttacggtaac
     2701 ctaattgttc agccagcgtc gctaatgtcc cgtaactggc aatctcataa tgctcgactt
     2761 tctgcgctgc ggcaatcagt gcggcatcac gcacttcgtt tttctcggta ctttcgatga
     2821 cctcattagc ttcttcaata agaccttcca ttgccacaca tttcatgcgc ttaattttca
     2881 ggttcgattc ggactccaca acttgatcaa tacgttcaat ctgtccatga gtttcctcga
     2941 ggtgcgcatg aaaagcctga cttaattttt catttgatgt tgctcttgcg agttttgcca
     3001 gtgcccgggt taattgtttt tctgcgctgt aggtatctga aagcaggtga ataaatacat
     3061 cttcaatggt cttcatattc atatttttct ccagtgaaat cactgcgagc catacggccc
     3121 gcagatgcaa ttatctttat cagctgatat taataattaa cggtgaatca ggatttgcct
     3181 gatttattac caccgctttg ttgaccgcct tttttacccg cttcagatgc gcgttgcgga
     3241 tcatttttaa aattaccgcc gctatgctga ccgcctttac ggcctgcgtc ggatgccttc
     3301 tcacggtctt cggcgaaatt tcctgaacca ccacgatgtt cggccatgtt atttctcccg
     3361 ttgcgttgca ttgtttcatt aatatgagtg ttgtgtgtcg acactcatta aaattagtcg
     3421 ctaatgagaa ttagtcaaat taagcgcaac gagaagatag agggaaaata tattttgagg
     3481 aacattctgg atatattaac aattacctga ggaataagtg acttagagaa aattattgat
     3541 tttactggtg ttatgttgcg ggattaattt gtttgattat attactgttg ggcggaaaaa
     3601 tgacgtaagt tgacgctcga ccggggtaag cgaaacggta aaaagataaa tattaaatga
     3661 atttaggatt tttccggctt cattaaagaa agttaaaatg ccgccagcgg aactggcggc
     3721 tgtgggatta actgcgcgtc gccgctttca tcggttgtac aaaaactttc agtgccgcca
     3781 gcattttctc tggctcatta atatgttgct cgatgatttt aacaatggcc gaaccagaaa
     3841 tcgcgcccgc agctcctgca tcaatcgctg cttttacctg atccggggcg gaaataccaa
     3901 atccctgcaa tggaggtgca gcgttgtact ctttcagctt cgcaaccaga tgattgaggg
     3961 gtaacgcggc gcggttttct gcgccggtca cgcctgctcg tgacagcaaa taggtgtaac
     4021 cacgaccgta agaggctatc tggcgcagca ggtcgtcatc ggcatttggc gggcagatga
     4081 agataggtgc gacattatga cgcaacgcgg cctggcggaa gggcgcggac tcttcaactg
     4141 gcacatcggc aaccagcacc gaatcgacgc cgactttttc gcactgggca taaaactcat
     4201 caatgccttt gttaaacacc agattggcat acatcaacag gccaatggga atggtcgggt
     4261 gtttctggcg aatcagtgcc agcatttcaa aacattgtgc cggagtcaca cctgccgcaa
     4321 aggcgcgcag agtggcgttt tgaatcgtcg ggccatccgc cagtgggtcg gagaagggga
     4381 tacctaactc cagcgcgtca gcaccggctt caattagcgt atcgataatt ttcaatgact
     4441 gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa tgcgccttct ttgcgctcct
     4501 tcaactgggc aaacagagat tcgtagcgtt ccatcagatt tcccctcgtg ctttcaaaat
     4561 atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa aggttaacca ccagtagctg
     4621 ctctttatcc gggttttcgc gcatcatttt caacgcatgg gccagggcgt gggaggattc
     4681 cagcgccggg atgatccctt cgtgcaggca cagcgttttg aaggcttcaa gggcttcatc
     4741 atcggtaata gacacgtaat cagcgcgtcc agtgctgtta agatacgcgt gttgtgggcc
     4801 gacagacggg aaatccagtc cggcggagat ggagtaagat tcttcaatct gcccgtcttc
     4861 ggtttgcatc atcggcgctt tcataccgaa atagataccc acgcgaccat gttttagcgg
     4921 tgcgccgtgc tcgccagttt cgataccgtg accacctggc tccacaccaa tcaggccgac
     4981 gttggtttca ttgatgaaat cagcaaacat gccgatggca ttcgaaccgc cgccaacaca
     5041 ggcgataacg gcatccggca ggcgaccttc tctttccaga atctgcgctt tggtttcttc
     5101 gccaatcatc cgctgaaact cacgcacaat ggtcggataa ggatgcgggc cagctgcggt
     5161 gcccagcata tagtgcgcgg tttcgtaact accggaccag tcgcgcagcg cctcgttaca
     5221 ggcatctttc agcgtcgcgg aaccgctatg caccgggatc acttccgcac ccattaagcg
     5281 catacgaaaa acgttaggcg actggcgttc aacgtctttg gcacccatat aaatacggca
     5341 tttcaggccg agcagggcgc tggcaagggc cgacgccacg ccatgctgac cggcaccggt
     5401 ttcggcgatg atttcggttt tacccatccg cttcgccagc aacgcctgcc ccagcacctg
     5461 gttagtttta tgcgcgccgc cgtgcagcaa atcttcacgc ttgagataca gcgtagtgtt
     5521 cgtcccggct gtaatgttct ggcatttggt cagcgcggtt ggacgcccgg catagttttt
     5581 cagcaggtcg ttgaactgag cctgaaattc aggatctttt tgcgcactga caaaagcttc
     5641 ttccagctgg cgcagagcag gcatcaggat ttgtggcacg tacatgccgc caaactcacc
     5701 aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt aatatgcgcg cagcgtctgg
     5761 aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt gcgactctac agcagaatta
     5821 aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc agttatctgc gcctaagccc
     5881 cccgccagca gaacgttgcc aagcgattga ccatttaata gtgaccagtc aaaacgttgc
     5941 ccgcttccac cctggccgtt gtctaaaaca tatttatcaa cgtgctgaaa ctcgcgggcg
     6001 ggcagggttt caccgacgct taatgctttc cagatggcaa catgtgctgg cagagcttca
     6061 cgcagcgtat cgatatacag ctgttcttca ttaccatgca gttgcactgc cgccagcgat
     6121 aacaccttag ctttgtccac cacatcggca atatcgtgat tgcggaacac gccaacatac
     6181 tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa cgttgacgca acgcggtgat
     6241 gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt cataagctgc tttagcatct
     6301 tgcccacgcg tcaggccaca tactttattc tcacccagca acacccggcg cacggcggcg
     6361 tgcaaatcgt catgggccat caacgccgaa ccaatcagaa aaccgttagc gaagtggctt
     6421 aactcgcgca cctgagcgta agtattgatg ccggattcgc tgattaccgt cacgttgtgc
     6481 cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa tcgacaaatc acgcagatcg
     6541 cggttgttga tgccaacgac ctttgccccc aatgcaatgg cacgctccag ttcctcttca
     6601 ttactgactt cggtcagcac acccatctcc agactgtggg cgacggcggc aagctggcga
     6661 tattgttcgt catccagtac tgaaagcatt aataagcagg catcagcctg gtaatagcgc
     6721 gccagataga tctggtaagg atcgataatg aagtctttac ataaaatcgg ctgcggggcg
     6781 atttggctga cgatggggag gaaatcaaag ctcccctgaa aatatttctc atcagtcagc
     6841 accgaaattg ccgaagcgta atgtttataa atggcggcaa tgcgtgctgg atcgaaatca
     6901 tcacggatca cgccttttga cggcgacgat ttcttgcact ccagaataaa cgccgtgcgc
     6961 gcaccctgta gcgcatcata aaaatgtcgc gtgctcggct gaacctcatt ctgaaaactg
     7021 gccagcggtt gctgctgttt gcgggtttct acccaaatcg ccttgtctgc gacgattttc
     7081 gctaaaacgg tttgcatcat ttaccctcgt gccgccagtg cggtaactct gtcgtaagcg
     7141 gaaccactgc gcagtacctc aagaacggtt tgcgcattgg cttgcagatc ttcatggcca
     7201 tgcaggcgca ttaacatggc gacgttcgca gcgacggctg cttcatgggc ggcgtcgcct
     7261 ttaccttgta acaagcgtgt taaaatgtca cggttttctt ccggtgttcc gcctgccagt
     7321 tgctcctggt ggtagggagt caggccaaaa tcttcagcgg tcaattgata gctcttaatt
     7381 tcgccgtcat gcagctcggc aacgattgtc ggcgcgtgta atgaaacttc atccatcccg
     7441 ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc gcaaggtttc ggcaatcggc
     7501 agcaccagtt ccggactata aacaccaatt aacgccagcg gcggatgcgc cgggttaatc
     7561 aatggcccca gcacattgaa cagggtgcgg gttttcagtt gctggcgaac cggcatcgcg
     7621 tggcggaatc cggtgtgata cttcggcgca aagaggaaac atacacctaa ctcatccagc
     7681 gcctggcgcg atttatcggc gttcatatca agattaatac cgaacgccgc cagcagatcg
     7741 gacgaaccag atttactgga gacgctacgg ttgccgtgtt tcgccacttt cagcccacag
     7801 gccgcggcga caaacgcact ggcggtagaa atattgatac tgttgctgcc gtcaccgcca
     7861 gtaccgacga tatcagcaaa cagataatcc gggcgcggga acggcgcggc gttttccagt
     7921 agcgcggttg ctgctccggc gatctcgttc gggtgctcac cgcgaatttt catgctcacc
     7981 agcgccgccg ccagttgttc cggcttcagc tcgccacgca ccaccgctga aaacagctgg
     8041 tggctttctt gttggctaag cgtctgcgcc tgatacagtt tttccagaat cggttgcagc
     8101 gtgttggctg gctctagttt ctgctgcgcc caggccagcg tttgttccag caggcgagcg
     8161 ccctgggtgg tgagaatgga ttccggatgg aactggaatc cacaaacgcg atccgcatcg
     8221 tgacgtactg ccatcaccat gccattaaaa tgggcgttga tggttaaacc ggccggaatg
     8281 ttactgccaa ccagcgagtg ataacgcgcc accggcagcg ggtttgttaa tccggcaaac
     8341 atcgcctgac cgtcatgttc aatgctcgac gctttaccgt gaagaatttc gcccgcctga
     8401 ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc cgaggcaaat gccaataatg
     8461 ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac aaccggcttc gctcggcaca
     8521 ccggggccag gagaaagcat cagtaccgga ttgctcatcg tcgccaggcg ttcaattaag
     8581 gtctgcgccg gaatatggtt gcggtaaatc accacattat gcccattgct gcgcaactga
     8641 tctgccaggt tgtacgtaaa agagtcgata ttatcgagca gcagaatgtc agccatcaga
     8701 aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag tacagcgcgg gctttattac
     8761 gggtttcgtc ggcttccgac tgcggaacag aatcaaggac tacgccagca ccggcttgca
     8821 cggtggcgat accgttttcc accagcgccg agcggatcac aatgcaggtg tcgagatcgc
     8881 catgcgcggt gaaataacct accgcgccgc cgtagctgcc gcggcgacga ccttccgcct
     8941 cggcaattaa ctgcatagcg cgtactttcg gcgcaccact taacgttccc atattcatac
     9001 aggcgcggta agcgtgcagg gcgtcgagat cgtggcgcag ctcaccgacc acgcgggaga
     9061 ccaagtgcat cacgtaagag taacggtcaa ctttggtgag gtcggcgacg tagcggctgc
     9121 cgggggtgca aatgcgtgcc agatcattac gggcgagatc caccagcatc agatgttcag
     9181 aaagctcttt atgatcggta cgcatttcca gttcgatgcg gctgtcgagg tctctgtcca
     9241 gtgaaccatc ggcacgacga ccgcgcggtc gtgttccggc aatcgggtag atctcaatct
     9301 ggcggctggt ggcgtcatac ttgagcgaac tttccggcga cgcgccaaac agggtgaaat
     9361 cattatcctg cataaaaaac atgtacgggc tgggattact ctttttcagc acgtaatagg
     9421 ccgccagcgg tgacgggcag ggcagagaga aacggcgaga tggcaccacc tggaaaattt
     9481 ctccggcgcg aatcgctttt tgcaacaaac gcactacgcc accgaactct tcatcgctct
     9541 ggttacattc acaacgcata tgcggcacgg aaaccaccgg cagcggtggc gcggtttcgg
     9601 tcagttgctg gcgaagatcg ttcaggcgag cagtgagacg ttgtttttct tcttcattcg
     9661 gagcaaacag gctggcctga atgcgggtgc tttttttctg atggtcaatc accatcagcg
     9721 tttcagcgag ataaaaacag aaatcaggac agttattttc cgctgacaat tgcggtaaat
     9781 cttcaaaccc cgccacaagg tcataagaga acaggccgcc gaagaacatt gcttctcgtt
     9841 cttccttcgg tacattcagc atattctgca ataagcggaa agcgtcaaaa accgaaaggg
     9901 agcataagcg agcgtcttca tccagcagtg gactgacagg ggggaagcgc agcacacggc
     9961 agtttggtaa ttgttcattt tccatacccg caggcagggc gttatccagt agtgtcagca
    10021 gggcttcgcc gttgccggaa agtgcctgga ttgtgacagt gtcacctaaa gctgtaatgc
    10081 gcagcgcact gtctaccagc agcaggcttt ttaaatcatc tttgctgtcg atatctgcgg
    10141 attccagcag cagcgttgcc ggacgatccc cacacaactg gtgaaaaagc gcggtgggat
    10201 tgtcgcgata agcgccttcg caggttagca gttcgagagt cggtttttgt gtttgcattg
    10261 ttattctcta attttgttca aaaaaaagcc cgctcattag gcgggctggg tatctgattg
    10321 ctttacgcat ggtgaataca ctgcccgttt caggaagtgc gccaccaacc tttcagtacg
    10381 aaaattgctt tcattgtcga tacccttttt acgtgaactt gcgtactagt taactagttc
    10441 gatgattaat tgtcaatagc tcatttcaga atatttgcca gaaccgttat gatgtcggcg
    10501 caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg cagtgattta
    10561 cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag cattggtgca
    10621 ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc gaccatgaca ccacagctgc
    10681 aatcgcgcca gcaagagagg aaatttcacg ttcgggactg gcgttgaatc ttattcccgg
    10741 cgtggaaatt tccacggtct gggaaaatca tgaaattcat attgtcgggc tgaatattga
    10801 tattactcat ccgctaatgt gtgagttcct cgcgcagcag acagaacggc gcaatcagcg
    10861 ggcgcagctg attgccgaaa gacttgaaaa agcgcaaata cctggggcgc tggaaggcgc
    10921 acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat tttgcgcgtt tccttgttga
    10981 gtgcggcaaa gccagttcaa tggcggatgt ctttaaaaag tatctggcgc gcgggaaaac
    11041 cggatacgtt ccgccacagt ggtgtacaat agaacaagct attgatgtca ttcatcattc
    11101 tggcggtaag gcggtgctag ctcatcctgg gcggtacaat cttagtgcta aatggctgaa
    11161 aagattggtt gcgcattttg ccgaacacca cggtgacgcg atggaagtcg cgcagtgcca
    11221 gcaatcgccc aatgaacgta cccagctggc ggcccttgcg cgtcagcatc atttatgggc
    11281 atcacaagga tctgattttc atcagccatg cccgtggatc gaactgggtc gtaaactctg
    11341 gctgcccgca ggcgttgaag gcgtctggca gctatgggaa cagccgcaga acaccacaga
    11401 gagggaatta tgagccagtt tttttatatt catcctgata acccacagca acgtctgatc
    11461 aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg tttatccaac tgattccggc
    11521 tatgcgctcg gctgtaaaat tgaagataaa aacgcgatgg agcgtatttg tcgtattcgc
    11581 cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg atctttctga actctcgacc
    11641 tattcatttg ttgataacgt tgcgtttcgt ttaatgaaaa acaacacgcc gggcaactat
    11701 accttcatct tgaaggggac gaaggaagtg ccacgccgtc tgttgcagga aaaacgcaaa
    11761 accatcggta tgcgtgtgcc ttctaacccg attgcgcagg cgttgcttga agcactgggc
    11821 gaaccgatgc tttccacttc gctaatgctg ccaggcagcg aatttaccga atccgatccc
    11881 gaagaaatta aagaccgtct ggaaaaacag gtagatttga ttattcacgg cggctatctc
    11941 gggcagaaac cgacaacggt tattgatctt accgatgata agccggtcgt ggtgcgtgaa
    12001 ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact ctgtatacta cgcggcctta
    12061 aaatggcgct atacggcgcc attgattctc ctgttcgacg cctgtgaagg cgacactaaa
    12121 aggaagctct atgagcgaaa aattacagaa agtgctggcc cgcgctggcc acggctcccg
    12181 ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt gttgatggca aaattgctaa
    12241 actcggcgat cgcgttgaag ttacccctgg gctgaaaatt cgtatcgatg gtcatctgat
    12301 ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg gcctattaca aaccggaagg
    12361 tgagttgtgt acccgtaacg atccggaagg gcgtccgacg gtgtttgacc gtctgccaaa
    12421 gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac gttaacacct gtggtctgct
    12481 gctgttcacc accgatggtg aactggcaaa ccgtttaatg catccaagcc gtgaagttga
    12541 acgtgaatac gccgtgcgtg tatttggtca ggttgacgac gcgaaactgc gtgatttgag
    12601 tcgtggcgtg caactggaag atggtccggc agcttttaaa accatcaagt tcagcggcgg
    12661 cgaagggatc aaccagtggt acaacgtgac tctgaccgaa ggccgtaacc gtgaagttcg
    12721 tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg atccgtgttc gttacggtga
    12781 tatcccactg ccaaaaggtc tgccacgcgg tggctggacg gagctggacc tcgcccagac
    12841 taactatctg cgcgaactgg tggagctacc gccagaaacc agctctaaag tcgctgtaga
    12901 aaaagaccgt cgtcgcatga aggcgaatca gattcgtcgt gcggtgaaac gtcacagtca
    12961 ggtgagcggc ggtcgtcgtt ctggcggacg taataacggt taatcaaagt atgccggacg
    13021 tcatatccgg catttttaca gattagtaat caatccctat ctgcgctttt acaccggcat
    13081 caaacgcatg tttgacgggg cgtaattcac ttaccgtatc tgccagttca agaatatccc
    13141 gatgacaacc acgacccgtg ataatcaccg tctgttgatg tggacgttca tttaacgcct
    13201 gcaccacttc ttccagtggc aaatagtcat acgccaccat atacgtcagt tcatcaagca
    13261 aaaccatatc cagtgaggaa tcagcaagca tccgctttgc atgttgccag acttcgcggc
    13321 aggcggcggt atcagactcg cggttttgtg tatcccaggt aaagcccgtt gccatcacct
    13381 gaaactcaac gccatgtggc tccagcagat tgcgttcgcc attaggccag gtgcctttaa
    13441 taaactgcac gacacctact ttttttccgt gaccaactgc gcgtgttgcc gtaccaaatg
    13501 ccgcggtggt tttgcctttt ccattgccgg taaagacgat gataataccg cgttcatcct
    13561 gggcctgggc cacacgagca tctacttttt ctttcactcg ctgctgacgc tgttggtagc
    13621 gttcatcact cattgggaaa ttcctggttt acggcccggt tgggcgtcaa aggtcatgcc
    13681 ggttttacgg cggctgtcat cgcccatcag ccagaggtag agcggcatga tatcagcggg
    13741 tgttttaagt ttctgtggat cttcggtcgg gaaggcgctg gcacgcattg cggtgcgcgt
    13801 accgcctggg ttaatgcagt tgacgcgcag gcgctgctga tattcatcgg ccagtacctg
    13861 catcatccct tcggtggcaa atttcgacgc tgcatatgca ccccagttag ctcgtccctg
    13921 acgtccaacg cttgatgaag taaagaccag tgaaccggcg tccgatttga gtaataaagg
    13981 aagcagcgcc tgggtgagca aaaaggtggc attaacgttg acctgcatga cgtcctgcca
    14041 gacctgcgga ttttgttcgc tcattgggca aacatcgccg agcaatccgg cattatgcaa
    14101 aacaccatcc agacgcggat aattaacggt aatgcgctgt gccagttgtt ggcaatcttc
    14161 ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc tgacgtccag tttcttcgtt
    14221 tatgtggctg gctacctgac gtaatttttc ttcattacgg cccaacagaa tcactgtcgc
    14281 accatagcgt gcatacgtca tcgcggcttc acggccaatc ccatcgctgg ctcccgtcac
    14341 caggataatg cgatcattga gtaaatcttg ttttggctgg taatgcatgg ctactcctca
    14401 acgacgttgt ctgtcgcgct gccagtgtac tttatgactt tggggcttta tgcctgaaac
    14461 agaagggcat ttcaatcagc ctggggcgat aacaccgcaa aatggatact ttgtcatact
    14521 ttcgctgcaa taacatctct gcgagacggc ttaacatgcc tgttgtaaac tgtgagccaa
    14581 agcgttgttt aaccaaggtg gggactcgtg gaattgttgt ctgaatatgg tttgtttttg
    14641 gcgaaaatcg ttaccgttgt gctagcgatt gcggcgattg ccgccattat tgtcaatgtt
    14701 gctcaacgta ataaacgcca gcgtggcgag ttacgggtca acaatctcag cgaacagtat
    14761 aaggagatga aagaagaact ggccgcggcg ctgatggact cacatcagca aaaacagtgg
    14821 cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag cagcaaaagc gaaagccaaa
    14881 ctgggcgagg tggcaactga cagtaaacct cgcgtctggg tgctggattt taaaggcagc
    14941 atggacgccc atgaagtgaa ctcgctacgt gaagagataa cggcggtact cgcagcattc
    15001 aaaccgcagg atcaggttgt gctccgtctg gaaagccctg gtggcatggt gcatggttac
    15061 gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa acattccttt aactgttacg
    15121 gtagacaaag tcgctgccag cggcggttac atgatggcct gtgtggcgga caaaattgtt
    15181 tccgcaccgt ttgctattgt gggttccatt ggggtggtgg cgcaaatgcc caactttaac
    15241 cgcttcctga aaagcaaaga tattgatatc gaactgcaca ctgccgggca gtataagcgt
    15301 actctgacct tgctgggtga aaataccgaa gaagggcggg agaaattccg cgaagagttg
    15361 aacgaaacgc atcagttgtt taaagatttt gtgaagcgta tgcgtccgtc tctggatatt
    15421 gaacaggtgg caacgggtga acactggtac ggacaacagg cggtagagaa aggcctggtt
    15481 gatgaaatca acaccagtga tgaagttatt cttagcctga tggaaggccg tgaagtggtt
    15541 aatgtacgct atatgcagcg taaacgactc attgaccgat tcaccggcag cgcggcagag
    15601 agcgccgatc gattgttgct acgctggtgg cagcggggtc aaaagccatt gatgtaaaag
    15661 acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa ccagatgata tttttctgaa
    15721 agcacatggg ccaggtgttt gaacatatta aacaccgcgg tgcttttggc tgttggcaat
    15781 ccttgttcat ctaaaaagta gtcgccggta aataccagca cgccattacg ctgcgtgacg
    15841 ccggtggctt caatccctgc gagcgtatcc tcatgctcac gaatgatttt gttggcctct
    15901 ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt tcattattta ctccttaaac
    15961 aaggacatta gtctacgcca ggcatggctt gcagacaaat ataccacgct ggtggcaaga
    16021 gcgccttact ggtaactttg gattttgcat gctaataaag ttgcgtatcg gattttatca
    16081 ggtacagtgt gacgctttcg tcaatctggc aatagatttg cttgacattc gaccaaaatt
    16141 ccgtcgtgct atagcgcctg taggccaaga cctgttaact cagtcacctg aattttcgtg
    16201 aacagagtca cgacaagggg ttgatatccg cagagaacga gtccatatcg gtaactcgtt
    16261 gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa gaatcaaatt aggtaaggtg
    16321 aatatgggta aagctcttgt catcgttgag tccccggcaa aagccaaaac gatcaacaag
    16381 tatctgggta gtgactacgt ggtgaaatcc agcgtcggtc acatccgcga tttgccgacc
    16441 agtggctcag ctgccaaaaa gagtgccgac tctacctcca ccaagacggc taaaaagcct
    16501 aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg ttgacccgtg gcacaattgg
    16561 gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg tctctgaact gaaacaactg
    16621 gctgaaaaag ccgaccacat ctatctcgca accgaccttg accgcgaagg ggaagccatt
    16681 gcatggcacc tgcgggaagt gattgggggt gatgatgcgc gctatagccg agtggtgttt
    16741 aacgaaatta ctaaaaacgc gatccgccag gcatttaata aaccgggtga gctgaatatt
    16801 gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc gcgtggtggg gtatatggtt
    16861 tcgccgctgc tatggaaaaa gatcgctcgt ggtctgtctg ccggtcgtgt gcagtcggtg
    16921 gcggttcgcc tggtggtcga gcgtgagcgt gaaattaaag cgttcgtgcc ggaagagttc
    16981 tgggaagtcg atgccagcac gaccacgcca tctggtgaag cgttggcgtt acaggtgact
    17041 catcagaacg acaaaccgtt ccgtccggtc aacaaagaac aaactcaggc tgcggtaagt
    17101 ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag acaaaccgac aaccagtaaa
    17161 cctggcgctc cttttattac ctctacgctg caacaagctg ccagcacccg tcttggattt
    17221 ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg aagcaggcta tatcacttac
    17281 atgcgtaccg actccactaa cctgagtcag gacgcggtaa atatggttcg cggttatatc
    17341 agcgataatt ttggtaagaa atatctgccg gaaagtccga atcagtacgc cagcaaagaa
    17401 aactcacagg aagcgcacga agcgattcgt ccttctgacg tcaatgtgat ggcggaatcg
    17461 ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt taatctggcg tcagttcgta
    17521 gcctgccaga tgaccccagc gaaatatgac tccacgacgc tgaccgttgg tgcgggcgat
    17581 ttccgcctga aagcacgcgg tcgtattttg cgttttgatg gctggacaaa agtgatgcct
    17641 gcgttgcgta aaggcgatga agatcgcatc ttaccagcag ttaataaagg cgatgctctg
    17701 acgctcgttg aacttacacc agcccagcac tttaccaagc cgccagcccg tttcagtgaa
    17761 gcatcgctgg ttaaagagct ggaaaaacgt ggtatcggtc gtccgtctac ttatgcgtcg
    17821 atcatttcga ccattcagga tcgtggctac gtgcgagtag aaaatcgtcg tttctatgcg
    17881 gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa atttccgcga gttaatgaac
    17941 tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg tggcaaatca cgaagcagag
    18001 tggaaagctg tactggatca cttcttctcg gatttcaccc agcagttaga taaagctgaa
    18061 aaagatccgg aagagggtgg tatgcgcccg aaccagatgg ttctgaccag cattgactgc
    18121 ccgacttgtg gtcgcaaaat ggggattcgc acagcgagca ccggggtatt ccttggctgt
    18181 tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca ccattaacct ggtgccggaa
    18241 aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa ccaacgcgct gcgcgcaaaa
    18301 cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc tcatcgatcc gaaacgtaag
    18361 ttgcatgtct gtggtaataa cccaacctgc gacggttacg agatcgaaga gggcgaattc
    18421 cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa aatgtggctc tgaaatgcac
    18481 ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca acgaagagtg taaaaacaca
    18541 cgtaagattt tacgtaacgg cgaagtggca ccaccgaaag aagatccggt gccattacct
    18601 gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc gtgacggtgc tgccggtgtg
    18661 ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc gtgcgccact ggtggaagag
    18721 ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt atctggccga tgcgccacag
    18781 caggatccgg aaggtaataa gaccatggtt cgctttagcc gtaaaaccaa acagcaatat
    18841 gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag cattttatgt tgatggcaaa
    18901 tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt tttataaaca aagggtcgcg
    18961 aaagcggccc ttttttattg catattattt tttcttcaca cctatacact aaggctataa
    19021 atgatatagt ggttatagtt agcacctttt ttattattaa atcgtattag tcacccgcca
    19081 ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg atggtttaac atgaaattac
    19141 aacaacttcg ctatattgtt gaggtggtca atcataacct gaatgtctca tcaacagcgg
    19201 aaggacttta cacatcacaa cccgggatca gtaaacaagt cagaatgctg gaagacgagc
    19261 taggcattca aattttttcc cgaagcggca agcacctgac gcaggtaacg ccagcaggac
    19321 aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt cgatgccata aaatcggttg
    19381 ccggtgagca cacctggccg gataaaggct cgctgtatat cgccaccacg catacccagg
    19441 cacgctacgc gttaccaaac gttatcaaag gctttattga gcgttatccc cgcgtttctt
    19501 tgcatatgca ccagggctcg ccgacacaaa ttgctgatgc cgtctctaaa ggcaatgctg
    19561 atttcgctat cgccacagaa gcgctgcatc tgtatgaaga tttagtgatg ttaccgtgct
    19621 accactggaa tcgggctatt gtagtcactc cggatcaccc gctggcaggc aaaaaagcca
    19681 ttaccattga agaactggcg caatatccgt tggtgacata taccttcggc tttaccggac
    19741 gttcagaact ggatactgcc tttaaccgcg cagggttaac gccgcgtatc gtcttcacgg
    19801 caacggatgc tgacgtcatt aaaacttacg tccggttagg gctgggggta ggggtcattg
    19861 ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt gcgtgttgat gctcacgata
    19921 tcttcagcca cagtacaacc aaaattggtt ttcgccgtag tactttcttg cgcagttata
    19981 tgtatgattt cattcagcgt tttgcaccgc atttaacgcg tgatgtcgtt gatgcggctg
    20041 tcgcattgcg ctctaatgaa gaaattgagg tcatgtttaa agatataaaa ctgccggaaa
    20101 aataatttct tgcgttattt tcggcacctt ttatgtagcg aaggtgccgg a
//
LOCUS       DAZFJF010000084        20028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFJF010000084 DAZFJF010000000
VERSION     DAZFJF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.084"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..335
                     /locus_tag="ACHKAU_003308"
     CDS             66..335
                     /locus_tag="ACHKAU_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5532311.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            304..1422
                     /locus_tag="ACHKAU_003309"
     CDS             304..1422
                     /locus_tag="ACHKAU_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO5532312.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKARMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGITPPAGKTPPTFHEQRSLSE
                     RLYRTQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV"
     gene            complement(1499..1966)
                     /locus_tag="ACHKAU_003310"
     CDS             complement(1499..1966)
                     /locus_tag="ACHKAU_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFO5532313.1"
                     /translation="MPDQKESENTKLTCEEQKDNELVSRVIENPEVLNRVLDSPQVRA
                     IVCQHFQGPVPPPSMLKKYDQLVPGLANRLVELTEKEQAHRHKTVADSIDIARDGQTK
                     AFWLAILIILAATVFGVMGETVLAGTLVSIDLVALVTAFIVGKHYSKQEPDQD"
     gene            complement(1944..2147)
                     /locus_tag="ACHKAU_003311"
     CDS             complement(1944..2147)
                     /locus_tag="ACHKAU_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532314.1"
                     /translation="MKRKSVALLQAIGSVIALSPTVVSANGMPANPVSALRQDAAAIR
                     GDFQKAMVSVNVEKLNNARSKRK"
     gene            2456..3250
                     /gene="potC"
                     /locus_tag="ACHKAU_003312"
     CDS             2456..3250
                     /gene="potC"
                     /locus_tag="ACHKAU_003312"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO5532315.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            3247..4293
                     /gene="potD"
                     /locus_tag="ACHKAU_003313"
     CDS             3247..4293
                     /gene="potD"
                     /locus_tag="ACHKAU_003313"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO5532316.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            4351..4812
                     /locus_tag="ACHKAU_003314"
     CDS             4351..4812
                     /locus_tag="ACHKAU_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO5532317.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            4809..5597
                     /gene="ycfZ"
                     /locus_tag="ACHKAU_003315"
     CDS             4809..5597
                     /gene="ycfZ"
                     /locus_tag="ACHKAU_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5532318.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5736..6557)
                     /gene="cobB"
                     /locus_tag="ACHKAU_003316"
     CDS             complement(5736..6557)
                     /gene="cobB"
                     /locus_tag="ACHKAU_003316"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO5532319.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(6573..7484)
                     /gene="nagK"
                     /locus_tag="ACHKAU_003317"
     CDS             complement(6573..7484)
                     /gene="nagK"
                     /locus_tag="ACHKAU_003317"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO5532320.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(7513..8757)
                     /gene="lolE"
                     /locus_tag="ACHKAU_003318"
     CDS             complement(7513..8757)
                     /gene="lolE"
                     /locus_tag="ACHKAU_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO5532321.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKDVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8757..9458)
                     /gene="lolD"
                     /locus_tag="ACHKAU_003319"
     CDS             complement(8757..9458)
                     /gene="lolD"
                     /locus_tag="ACHKAU_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO5532322.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(9451..10650)
                     /gene="lolC"
                     /locus_tag="ACHKAU_003320"
     CDS             complement(9451..10650)
                     /gene="lolC"
                     /locus_tag="ACHKAU_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO5532323.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10912..11985
                     /gene="ycfT"
                     /locus_tag="ACHKAU_003321"
     CDS             10912..11985
                     /gene="ycfT"
                     /locus_tag="ACHKAU_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5532324.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            12113..15559
                     /gene="mfd"
                     /locus_tag="ACHKAU_003322"
     CDS             12113..15559
                     /gene="mfd"
                     /locus_tag="ACHKAU_003322"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO5532325.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15703..16665
                     /gene="ldtC"
                     /locus_tag="ACHKAU_003323"
     CDS             15703..16665
                     /gene="ldtC"
                     /locus_tag="ACHKAU_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO5532326.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16748..17005)
                     /gene="bhsA"
                     /locus_tag="ACHKAU_003324"
                     /gene_synonym="ycfR"
     CDS             complement(16748..17005)
                     /gene="bhsA"
                     /locus_tag="ACHKAU_003324"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO5532327.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            17246..17878
                     /gene="comR"
                     /locus_tag="ACHKAU_003325"
     CDS             17246..17878
                     /gene="comR"
                     /locus_tag="ACHKAU_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO5532328.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(17940..18479)
                     /gene="ycfJ"
                     /locus_tag="ACHKAU_003326"
     CDS             complement(17940..18479)
                     /gene="ycfJ"
                     /locus_tag="ACHKAU_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5532329.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(18706..20010)
                     /gene="ndh"
                     /locus_tag="ACHKAU_003327"
     CDS             complement(18706..20010)
                     /gene="ndh"
                     /locus_tag="ACHKAU_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO5532330.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT         5068 a         5163 c         4959 g         4838 t
ORIGIN      
        1 ttaatgttca accgccgcca gttgtcgttg atattctgca actggcgcgt tccggagtga
       61 tagccatgag cgaacagtac ctgataacgc tcgacgagtg gaaaccaaaa cggttcagtc
      121 tcccaataac aaacactacc ctggtgaaat acggaaaact aggatacatc gttccaagac
      181 cacaaaaaat tcgtgggcgt tggctgatag atcgccgagc agtatttgtt ggacctggtg
      241 aaacaggaat tgcgccggaa attcatactg gcgatgatga tgcactgaag gagattttaa
      301 ctcatgtcac cgaggccacg aaaaaacagc actgacgtag ccggacttta cgaaaaattt
      361 gatcgcagaa ctggcagagt ttactaccag tataaaaacc ctgtgactgg aaaatttcac
      421 gggctcggaa cagacaaagg taaggcagaa aaaatcgctt ccacagccaa tcagcgaata
      481 gctgcagcag aggctgaata tttcatgcgc aaaattgatg aaagtccgtc agcaacaaag
      541 cgtcggggta tcagattaaa ggcatgggtt gatcgatatc tgaaaataca ggacacgcga
      601 ctgaaaaatg gagatattgc agctacaact cacaaagaaa aagcccgaat ggctgcatac
      661 ctggtttccc gtctgggaaa ccacccattg aaagaactgg aagtaagaga ctttgcatta
      721 atactggatg agtggctgga taaagacatg gtcagcacag cgagagtaaa tcgtggatta
      781 tgggttgata tttataaaga agcacagcat gcaggggaag ttcctcctgg atggaatcct
      841 ccggaggcta cccgtaaacc gatccctaaa gtaactagag ccaggctcac cctggaagac
      901 tggcaaaaaa tttacaacgc aacgcctgaa aaacacttta tccgtaacgc aatgcttctt
      961 gcgattgtta ctggtcagcg ccgtgatgac atttgccaca tgcgtttttc agatgtgtgg
     1021 aacgaacact tgcatatcac ccagggaaaa accggaatgc gtctggcgtt accgcttaca
     1081 ctacgctgtg atgccattgg gataacgtta aaagaagtta ttgatgggtg ccgagacaga
     1141 atattaagtc catatctaat ccatagtcgg caccagaaac aaccaaagcc gatgagtaaa
     1201 gacaacctga gcgactactt tgccaaagca cgggatctgg ctgggataac tccaccagca
     1261 ggaaaaactc cgccaacatt tcatgaacaa cgctccctgt cagaacgact gtaccgcacg
     1321 cagggtattg atacaaaaac attactggga cataaagtcc aggcaaccac cgatcgttat
     1381 aacgatactc gtggtcagga atgggttaaa ttggttgttt gacgaaataa atgcagctga
     1441 aaaatgttga tattgctttg ctagtgtaaa aaccccggca tcaaccgggg ttcggagact
     1501 aatcttgatc aggttcctgc ttagaataat gttttccaac aataaatgcc gtaaccaatg
     1561 caacaagatc tattgaaacg agagttccgg caagaactgt ctcccccatg acgccaaaga
     1621 cagtggcagc taagatgatc aatattgcca accaaaaagc ctttgtttga ccatctctgg
     1681 caatatcaat gctatcagcc actgttttat ggcgatgagc ctgctctttt tcggtcaact
     1741 caacaagtcg atttgcaagc ccaggcacca gttgatcata ctttttaagc attgaaggtg
     1801 gcggaacagg cccctgaaaa tgctggcaaa caatagcccg cacctgcgga ctatccaaaa
     1861 ccctgtttaa gacctctgga ttttcgatta ctcgagaaac cagttcatta tccttttgtt
     1921 cttcacaagt gagtttggtg ttctcacttt ctttttgatc tggcattgtt cagcttttca
     1981 acgttaacgg ataccattgc tttttggaaa tcgcctctga tagccgcagc atcctgacgc
     2041 aaagcggaaa ctggattcgc tggcatgcca tttgctgaaa cgaccgtcgg gctcagggct
     2101 ataaccgagc caatggcttg taacaaagcc acacttttac gcttcatagt cactcctgag
     2161 ttcaatggga ttcaaaacgg attcaaagtg aatcttgata gctaattcta ataccacatt
     2221 atgtggtttt tcaacacgaa gaaatcacat caaaaatctc attcggaaag cgatatgggt
     2281 aaacaaactg gaatactttt ctgtcctagt aagctcaccc atgtataaaa atgctgtaaa
     2341 atgcgtggtc ctgttttggg gaaaagtttt ggagagattt tggagaaagg aaaaaattga
     2401 ataaattcaa aatcctgaaa gtgtcccgcc ttatgaataa aaaggtggaa ctcgaatgat
     2461 cggtcgactg cttcgcggcg gttttatgac cgctatctac gcgtacctgt atatcccaat
     2521 cattattttg attgtgaact cctttaacag ctcgcgcttt ggcatcaact ggcagggttt
     2581 taccaccaaa tggtatagcc tgctgatgaa caacgacagc ctgttacagg cagcgcagca
     2641 ttcactaaca atggcggtgt tttcggcgac atttgctacg cttatcggtt cactgacggc
     2701 agttgcgctg taccgttatc gctttcgtgg taagccgttc gttagcggaa tgctgtttgt
     2761 ggtgatgatg tcaccagata tcgtgatggc gatttctctg ctggtgctgt ttatgctgct
     2821 gggtattcag cttggcttct ggtcgctgct gttctcgcat atcaccttct gcctgccgtt
     2881 tgtggtggtg acggtgtatt cgcgcctgaa aggttttgac gtgcggatgc tggaagcggc
     2941 gaaagatctc ggtgccagtg aatttaccat tctgcgaaaa atcattctgc cactggcaat
     3001 gcctgcggtg gcggcgggct gggtgttaag ctttaccctg tcgatggacg atgtggtagt
     3061 ttcttcgttc gtcaccggac caagttatga aattctgccg ttaaaaattt attcgatggt
     3121 caaagtcggc gtatcgccgg aagttaacgc gctggcaacc atattactgg tgctgtcgct
     3181 ggtgatggtt attgccagcc agcttattgc tcgtgataaa acgaaaggta acacagggga
     3241 cgttaaatga aaaaatggtc acgccacctg ctcgcggcgg gtgctctggc actgggcatg
     3301 agcgccgctc acgccgatga caacaacacg ctgtatttct acaactggac cgagtacgtg
     3361 ccgccaggac tgcttgaaca gttcaccaaa gaaaccggta ttaaggttat ctattcgact
     3421 tacgagtcga acgaaaccat gtacgccaag ctgaaaacgt acaaagacgg tgcctatgac
     3481 ctggtggttc cttcaaccta ttacgtcgat aaaatgcgta aagaagggat gatccagaag
     3541 atcgacaagt cgaagttaac gaacttcagc aatctcgatc cagacatgct caacaagccg
     3601 tttgacccga ataacgatta ctccattccg tatatctggg gtgcgacggc gattggtgtt
     3661 aacggtgatg cggtggatcc gaaatctgtc accagctggg ccgatctgtg gaagcctgag
     3721 tacaaaggca gcctgctgtt gaccgacgat gcccgtgaag tgttccagat ggcgctgcgt
     3781 aagctgggct actccggtaa caccaccgat ccgaaagaga ttgaagctgc atataacgag
     3841 ctgaaaaaac tgatgccaaa cgtcgcagcg tttaactccg ataacccagc taacccgtac
     3901 atggaaggcg aagttaacct cggcatgatc tggaacggtt ctgctttcgt tgcacggcag
     3961 gcgggtacgc caattgacgt ggtgtggccg aaagaaggcg gcattttctg gatggacagc
     4021 ctggcgatcc cggcaaatgc caaaaacaaa gaaggcgcgc tgaaattgat caacttcctg
     4081 ctgcgcccgg atgtggcaaa acaggttgct gaaactatcg gttatccaac gccaaacctt
     4141 gcggcgcgta agctgttaag cccggaagtg gcgaacgata aaacgctcta cccggatgct
     4201 gaaaccatta aaaatggtga atggcagaat gacgttggcg cagccagcag catttatgaa
     4261 gagtattatc agaagctgaa agcaggacgt taattcagca agcgggcggt ggcattaccg
     4321 cccgtttttt atttagcaag gatgtggcta atgagtcagg acagtaaagt gttatcccgc
     4381 gcttttctcg gtatcggtct gatattgctc gttatttctg caatcatctt ttattaccat
     4441 tttactttta ccaaaagcgc ggttcataca gaaggaatca ttgttgatgc cgtttggtac
     4501 aacaatcact caaacgacgt cgatgataat ggttcctggt atccagtggt tgcctttcgc
     4561 cctacgccag attacacgct aattttcaat tcgaatatcg gtagtgattt ttatgaagat
     4621 agcgaaggtg acagagtcaa tgtctattat ccgccagggc atccagaaca agccgaaata
     4681 aacaatccct gggttaattt ttttaaatgg ggctttgttg gcatagcagg cattattttc
     4741 ggtagtgtcg gtctgataat cacgctcccc tccaccaaaa aatctcgcag gaaacggaag
     4801 tcctgcccat gaaaaaattc attatactgc tcagtttact gatactcctg cctctggtag
     4861 caaccagtaa accattgatt ccaataatga agacactttt tacggatgtt accggtactg
     4921 taccggatgc tgaagagatc gcacgtaaag ccgagctttt tcgccaacaa acgggaattg
     4981 caccgtttat tgtgatttta cccgacatta ataatgaagc cagtctcaga caaaatggta
     5041 aagcgatgct ggcgcatgcg tcatcttcat tgagtaatgt aaaaggaagt gttctgttac
     5101 tatttactac ccgcgaacca cggttaatta tgatcaccaa cggccaggtt gaaagtggtc
     5161 tggacgataa acatctcggc cttctgatag aaaatcacac gctggcttat ttaaatgcag
     5221 atctctggta tcagggaatc aataatgcat tggctgttct acaagcacag atattaaaac
     5281 aaccgacgcc gccactgacg tattatccgc atccaggaca acagcatgaa aatgctcccc
     5341 ccggcagcac caacacatta ggttttatcg cctgggctgc gacgtttatc ttattttcct
     5401 ggatcttttg ctacacgact cgttttattt atgcgttaaa atttgcagtg gcgatgacta
     5461 ttgccaatat gggttatcag gctttatgtt tgtatatcga taatagcttt gcgattacca
     5521 gaatatcgcc actctgggca gggctaattg gtgtctgtac gtttattgcc gcattgttat
     5581 tgacgagtaa acgataaaag aggccggaaa agtgcgcgtg tattatgccg gatggggcgc
     5641 gaacgcctta tccggcccac aaaacccgca aattcaatta attgcgtccc cttgtaggcc
     5701 tgataagcgt agcgcatcag gcaatgcttc ctgctttata atcccttcag caacttttca
     5761 acaaactcag gcaccacctg gcttgccggg ccgtaatatt tctcggcaaa ttcattaccg
     5821 acctgacttg gttcgagatt cagttccacg gtgtgcgcgc catgcagttt cgcttcgtga
     5881 acaaacccag ccgccggata aacatgcccg gaagtaccaa tggcaatgaa aatatcagcc
     5941 atcgacaacg ccatataaat ttcatccatg ccgagtggca tttcgccaaa ccaaacgacg
     6001 tgcgggcgca agggtgccgg gaactggcaa caatggcatt tatcttctgg cgtaacgtct
     6061 ccggtccagt cgagaacctg accactttgc gaacaacgaa ctttcagcaa ttccccatgc
     6121 atatgaatga cattggtatt acctgcgcgt tcatgcaggt tgtctatatt ctgcgttacc
     6181 agcaaaaagc gatcgccgag ggcgtcttgc agtttagcca gcgcaagatg cgcggcgtta
     6241 ggctgaattt ctggctgctg cagctgtcga cgacgggcgt tataaaacgc ttgcaccagt
     6301 tcaggatcgc gatcgaaacc ttccggcgtt gccacatctt caacccgatg ttcttcccac
     6361 aggccatctg cggcgcgaaa ggtacgaata cctgattccg cagaaatccc tgcccctgtc
     6421 agtacgagta ctcttggttt ttccattgct tccggcacca ctttatctct gaaaaaaata
     6481 cgctgacgca aacgctcgcg caggcggcgt ttatttttac gaaaacgact taaccgatga
     6541 ccccgacgcg acagcatagc aacctctgtt gtttaatcgg ttagatgtag gaaggccgca
     6601 ccacgcattc ctcccgcatc accgtggcgc gcgcgttcaa tgcgcggaac acgagctaca
     6661 ggtaagagat gacgaggcag cctgtccgcc agttgcgttg tgattgccgg gaaattcgat
     6721 aagccaccac caatgacgac caggtcaggg tcaacaatgg tcaggatatt tcccagacaa
     6781 accgctaata aatccagata acgctcaacg tgcgcccttg cctgctcatc gccttgatca
     6841 taaagcgcaa taatttcggg agcctgcaac ggttgatgat aatagtgttg atacagccac
     6901 gcaaaaccgc gaccagacag ataattttca atgcagccat gctgaccaca gccgcagcgg
     6961 cgtaacggga aatccaaccc catcatggtt aacgcatcaa ccggcagacg catatggcca
     7021 aactcgccgg taatgtagct tttaccggta atcggtttgc cgttgaaaat cagcccgccg
     7081 ccaacgccgg tgccgagaat caaccccatc accagtggat attgagtaaa ttcatcatcc
     7141 caggcttctg aaagggcaaa acagttggca tcgttatcaa ggcgtacatc gcgatcaaga
     7201 cgtgcgctca ggtcggcacg cagcggttta ccgctggcag cggggacatt ggcggcatac
     7261 agcgtaccat cttctgtttc cggcattccc ggaataccga tgccgacaga acctttacag
     7321 ccaaaacgtt ggtcagcttc agctaccagc tcacacactg catctaaaaa tgcgtcatag
     7381 ctgtcacgcg gtgtcggcac ccgcttttcc cactgcaact gccgaccgct atcaaacacg
     7441 ccaagcgcaa tttttgttcc accaatatca aacccgtaat acattgccgc tccttgtttt
     7501 aatgtactgc ctttactggc cgctaaggac tcgcgcaggg tcaatattac tggcgcgccg
     7561 cgccggatac caacttgcca aaagactcag caacaatgct gtgaccagta cgtagaagac
     7621 gtccagccag tgcaattccg atggcaggaa atcaataaaa tagatatcgc tggagaggaa
     7681 ctgatgaccg atcaactttt caatccactc aataatcggg gtaagttgca gtgaaaccac
     7741 cacaccgata atcaccccac acaggctgcc gaacagccct gccagcaatc cataccagac
     7801 aaagatggcg cgaattaaac catctttcgc ccccagcgtt cttaatactg cgatatcgcc
     7861 actcttgtct ttcaccgcca tcactaaggt ggagacgatg ttgaaacagg ccacgccaat
     7921 caccagtacc atcgccagat acataatggc gcggatcatc tggatatcgc gatacatata
     7981 gccgtaagta ccaatccaac ttttaatata aacatagctg ttggtcactt cacccgcatc
     8041 gcgtaccagc ttattggcgt tgaaaacatc cgtcatttta agggcaatac ctgacacgct
     8101 ggaacccata tcaagatatt gttgggcatc cgccagcggg atcatggcaa aactgtgatc
     8161 gagttgacca ctcaactgca aaataccggc aacgtgcaaa cgcacacgtt ttggctgcat
     8221 cagtttatgc tcgggattcg agttggggat cataatcgac acccaatccc cctgcttcac
     8281 tttcagcgca tccgccacgt ctttgccgat gataatttgc tgttcgcccg ctttaaaatt
     8341 gcgccacgca tcgccctgga caaacgaggg taatgcgctc agacgctgtt cctgttgcgg
     8401 gttaacgccc ttcacctgga ttgcgcgaag attcgcgcca ctttccacca gcccggtgaa
     8461 attgatatac ggcgcggcag cggcaatacc cggcactttt tgcacgtgat ccagtgcttc
     8521 ctgccagtta gtccacggct gatccaccgc ctcgatttca ccatgcggca ccaccgccag
     8581 aatacggttg ttcagttcgc gttcaaagcc gttcatcgcg cttaagccga cgatcaatac
     8641 cgccacgccg agggcaatgc caatggtaga aataacggag atcagcgaca ccatgccgcc
     8701 gcgccgccgt ccgcgactaa aacgcaggcc aattaataac gataaaggca tcgccattac
     8761 tccgccccca tcaggctcag ttccgccgtc agacgaccat cacgcatttc taactggcgg
     8821 ctcatacgtt tcgccagttg caggtcgtga gtaaccacca ggaaggcggt gccctgcaag
     8881 cgattcaatt ccccaagcaa ctggaagatg ctgtcggcgt tacgcgcatc gaggttaccg
     8941 gtaggttcat ccgccagtac caggcgcggg ttattcacca gcgcacgggc aatcgccaca
     9001 cgctggcgtt cgccgccaga aagttcagat gggcggtgat tcgcacgatg atccagcccc
     9061 accgctttta acatctcaag tgcacggctg ttgatttcag cgggcttttt cttgccaatc
     9121 agcagcggca tagccacgtt ttccagggcg gtaaaatccg gcagcaggtg gtgaaactga
     9181 taaataaagc ccagcttctg gttgcgcagc tctgctttcg ccgccgaaga cagtttgctc
     9241 atcggttgac cgttaaagat cacatcgccg gaggttggcg tatccagccc gcccaacaag
     9301 tgcagcaagg tgcttttacc ggaaccagag ctaccgacga tcgccatcat ttcaccttcg
     9361 ccgacgctga aactgacatt gtgcagcaca tcggtttgca cactgccttc ctgatagcgt
     9421 ttgcacaggt tgtcgcattg caacaggatc ttattcataa cgtaaagcct cagcgggttg
     9481 agtggcggca gcgcgccatg aagggtaaag cgtagacagc agcgcgatag ccatcgccac
     9541 cagcgcaata acaatgacct gtaaaggttc gatagcgacc ggcagcgccg cgccatcaag
     9601 caggacgccg attatcggca tcagattatt taactggctg gcaagcagtg cgccaagcgc
     9661 cgcaccgagg atcgcaccga taatcccggc gctggcccct tgcaccataa agaccatcat
     9721 gatttgtcgc ggagttaagc cttgcgtttg caggatcgcg acttcgccct gcttctccat
     9781 taccatcagc cctagtgagg taataatgtt aaacgccgca acggcgacaa tcaggctcag
     9841 cagcaagccc atcatatttt tttccatgcg tacggcctgg aacagctcgc ctttacggtc
     9901 acgccagtcc tgccatttgc tgccttcagg cagtttttgc tgacttaaag agtcaacttt
     9961 cagcggctca tccagccaca aacgccagcc ggtaatattg cctgccggat aacgcatcag
    10021 acgcgaggca tcctcaatat tcaccagcat ttcatagcca tcgacttcac tgttggcggc
    10081 gaaagtgcca atcacattga acaggcgctg gcttggaata cgccccatcg gcgtgaactg
    10141 gctggcagat ggtaccatca cgcggatttg atcaccgcga ttaacgccta gctgtgaggc
    10201 aagttgttcg ccgaggatga cattatattt ccccggctcg aggtcagttt gtttcacatt
    10261 gaccagatac ggtgtaagtg gatctttttg cgccgggtcg ataccgagca tcaccccgac
    10321 cgccacgctg cgcgcgcttt gcagtaccac atcaccggta gtaataggtg cgacgcgatt
    10381 aacgccgtcc agtttgactg ccgtttccgg gagttgctgc gggttaagag agccatgctc
    10441 agaagagaga attgcctgtg gcatcaggcc aaggatgttg ttttgcagct cgcgctcaaa
    10501 gccgttcatc actgacaata ctgtgaccag cgccatcacc ccgagggtaa tgccgatggt
    10561 agaaagccag gagacgaaac gaccgaagcg atccgctgca cgcccacgca tgtaacgcag
    10621 gccaataaat agagcgacag gttggtacat gaaatccgtc tggttgctgt agcaaagaca
    10681 cgagtatata agcgaaagca agatgggtaa acgtctgaac tgtaagtgat cgtcagggct
    10741 aatctgaaaa gcccccgtaa atcaatcggc taaattgcca tgttttatca tcaatcttgc
    10801 cgcctgatct taatctgaaa aatcatccaa tacagacaga tccccattac acctcgggtc
    10861 ataacccgca ggatcaaccg catgtaaatt aaaaaagagc gtgttgtcct gatgaaacaa
    10921 aaagagctat ggattaacca gatcaaaggg ttatgtattt gtctggtggt gatttatcac
    10981 tcggtcatta ccttttatcc gcatctgacc acttttcagc atccgttatc ggaagtcctg
    11041 agcaaatgct ggatctattt caatctttac cttgccccct ttcgtatgcc ggtttttttc
    11101 tttatatccg gctatttgat tcgccgctat atcgacagcg taccgtgggg aaattgtctc
    11161 gataaacgga tctggaacat cttctgggtg ctggcgcttt ggggcgtggt gcagtggctg
    11221 gcgttaagtg cgctgaacca gtggctggca cctgagcgcg atttaagcaa tgcctccaat
    11281 gccgcttatg ccgattccac cggtgagttc ctgcacggga tgatcaccgc cagcaccagc
    11341 ttgtggtatc tgtatgcgtt aattgtctat ttcgtggtat gtaaaatttt tagccgtctg
    11401 gcgctgccac tattcgcctt gtttgtactg ctgagtgtgg cggttaattt cgttcccacg
    11461 ccgtggtggg gaatgaacag tgtgatccgc aatttgcttt attacagcct tggcgcatgg
    11521 tttggcgcaa caataatgac ctgtgttaaa gaggtgccgt tgcgccgcca tctgctgatg
    11581 gcttctttgc ttaccgttct ggcggtcggt gcctggttgt ttactatctc gctgctgttg
    11641 tcgctggtat cgattgtggt gatcatgaag ctgttttatc aatacgagca acgattcggt
    11701 atgcgctcca ccagcctgct gaatgtgatt ggctccaaca ccattgctat ctacaccacc
    11761 catcgcattc tggttgaaat attcagctta actctgcttg cgcaaatgaa cgcagcacgc
    11821 tggtcgccgc aagtcgaact gacactcctg ctggtttatc cctttgtcag tttgttcatc
    11881 tgtactgttg cgggcttgct ggtaagaaaa ctttcacagc gtgcattcag cgatctgttg
    11941 ttctccccgc cttcactgcc cgctgccgtc agttactctc gttaaaccat cgccccagcc
    12001 ggggcgattc aatttgctaa accatgtcga ttacggataa tgaacagcat tgcttatcag
    12061 agatatgccc ccatatgttg aggcatatcc taacgagaat ctgacaaccg ttatgcctga
    12121 acaatatcgt tatacgctgc ccgtcaaagc gggtgagcag cgtctgctgg gcgagttaac
    12181 cggcgcagcc tgtgcaacgc tggtagcgga aattgccgaa cgtcacgccg gtccggtggt
    12241 actcattgca ccagatatgc aaaatgctct gcgtttgcat gatgaaatca gccagttcac
    12301 cgatcagatg gtgatgaatc tggcggactg ggaaactctt ccctacgaca gtttttcgcc
    12361 tcatcaggac attatctcct cgcgcctttc caccctttac cagctaccga cgatgcagcg
    12421 tggcgtactg attgttccgg tgaatacgct tatgcagcgc gtttgcccac acagttttct
    12481 ccacggtcat gcgctggtga tgaaaaaagg tcagcgcctg tcacgagatg cattacgaac
    12541 ccaactggac agcgccggtt atcgccatgt tgaccaggtg atggagcacg gcgaatacgc
    12601 cacgcgcggc gcgttgctgg atctcttccc gatggggagt gagctgcctt atcgtcttga
    12661 tttctttgat gatgaaatcg acagcctgcg ggtgtttgac gtcgacagcc agcgcacgct
    12721 ggaggaagta gaagcgatca atctgctgcc cgcgcacgaa tttccgaccg ataaagcggc
    12781 aattgaactg ttccgcagcc agtggcgcga taccttcgaa gtgaagcgcg atccggaaca
    12841 tatttaccag caagtgagta aaggcacatt acctgccggg atcgagtact ggcagccatt
    12901 gttcttcagc gaaccactgc cgccgctgtt cagttatttc cctgccaata ccttgctcgt
    12961 gaatactggc gatctggaaa ccagtgccga acgtttccag gctgacacgc tggcgcgttt
    13021 tgagaatcgc ggcgtcgatc cgatgcgccc gctgttgcca ccacaatcgc tctggctgcg
    13081 ggtggacgag ctcttctcag agctgaaaaa ctggccccgg gtgcagctaa aaactgaaca
    13141 tttaccgaca aaagccgcga atgccaattt aggtttccag aaactgccag acctggccgt
    13201 tcaggcacaa caaaaagcgc cgctggatgc gctgcgtaag ttcctcgaga ctttcgacgg
    13261 tccggtggtg ttctcggtag aaagtgaagg tcgccgtgaa gcgctgggtg aactgctcgc
    13321 acgaattaaa attgctccgc aacgcattat gcgtcttgat gaagccagcg accgtgggcg
    13381 ttatctgatg attggcgctg ccgaacatgg ttttgtcgat aaggtgcgta atctggcgct
    13441 gatctgcgaa agcgatctgc tcggtgaacg tgttgcccgc cgtcgtcagg attctcgccg
    13501 caccatcaac cccgatacac tgatccgtaa ccttgcggag ctgcatattg gtcagccggt
    13561 ggtccatctg gagcacggtg tcgggcgcta tgccggaatg accacgctcg aagcgggcgg
    13621 cattactggc gagtatttga tgctcaccta tgccaacgac gccaaactgt atgttccggt
    13681 gtcgtcactg catctgatta gccgttacgc aggtggcgcg gaagaaaacg ccccgctgca
    13741 taaacttggc ggcgatgcgt ggtcacgcgc gcggcagaaa gcggcggaaa aagtgcgtga
    13801 tgtggcggcg gaattgctgg atatctacgc gcaacgcgcc gccaaagagg gcttcgcgtt
    13861 taaacacgat cgtgagcagt atcagttgtt ctgcgacagc ttcccgtttg aaaccacgcc
    13921 ggatcaggcg caggccatta atgcggtact tagcgacatg tgtcagccgc tggcaatgga
    13981 tcgtctggtg tgcggcgatg ttggctttgg taaaacagaa gtggcgatgc gcgccgcttt
    14041 cctggcagta gataaccaca agcaggtggc ggtgttggtg cctaccaccc ttctcgcgca
    14101 gcagcattac gacaacttcc gcgaccgttt cgccaactgg ccggtacgta tcgaaatgat
    14161 ctcccgtttc cgcagcgcca aagagcagac gcaaatcctt gcggaagtgg cggaagggaa
    14221 aatcgatatt ctgatcggta cgcacaaact gctgcaaagt gacgtcaagt ttaaagattt
    14281 aggcctgctg attgtcgatg aagaacaccg cttcggggtg cgtcataaag agcgtattaa
    14341 agcgatgcgc gcgaacgtgg atattctgac gcttactgca acgccgatcc cacgtacgct
    14401 gaatatggca atgagcggaa tgcgtgacct gtcgattatc gccacgccgc ccgcccgtcg
    14461 tctggcagtt aaaacctttg tccgtgagta tgacagcctg gtggtccggg aggcgatcct
    14521 gcgtgaaatt ttgcgcggag gacaggttta ttatctctac aatgatgtgg aaaacattca
    14581 gaaagccgcc gaacggctgg cagaactggt gccagaagcg cggatcgcca tcggtcacgg
    14641 gcagatgcgc gagcgcgaac tggaacgggt gatgaatgat ttccatcatc aacgtttcaa
    14701 cgtgctggtt tgtacaacca ttatcgaaac cgggatcgac atcccgacag ccaacaccat
    14761 tatcattgaa cgcgcggatc acttcggtct ggcgcagctg caccagttac gcggtcgcgt
    14821 cggacgttca catcatcagg catatgcatg gttgctgacg ccgcatccaa aagcgatgac
    14881 taccgatgca caaaaacgtc ttgaagcgat tgcctcgctg gaagatctcg gggcaggttt
    14941 tgcgctggca acgcacgatc tggagattcg cggcgcgggt gaactgcttg gcgaagaaca
    15001 aagcggctca atggaaacca tcggtttctc gctgtatatg gagttgctgg aaaacgccgt
    15061 cgatgcactg aaagccggac gcgagccgtc gctggaagat ctcaccagcc agcaaacaga
    15121 agtcgagctg cggatgccgt cgctattgcc agatgatttc attcctgacg tgaatacgcg
    15181 tttgtcgttc tataaacgta ttgccagcgc caaaacggaa aacgaactgg aagagatcaa
    15241 agtcgagctt atcgatcgct tcggcctgct gccggatccg gcgcgtaccc tgctggatgt
    15301 tgcccgtctg cgccagcaag cgcagaaact ggggatcagg aagctggaag gtaatgagaa
    15361 aggcggggtg atcgaatttg ccgagaagaa tcacgttaat ccggcctggt tgattggttt
    15421 gctgcaaaaa cagccgcagc attaccgcct tgatggtccg acgcgcctga aatttattca
    15481 ggatttgagt gagcggaaaa cgcgtatcga atgggtacgc cagtttatgc gtgaactgga
    15541 agagaacgcg atcgcttaat ggtaaatcgg gggcgtttct gcgcccccat ctgcaacatt
    15601 tacaaattct ttgcacttcc ctgcactatc cgacactgtc accatccata attcaggctt
    15661 atctgtttat tacaataacc ttatatttat tatggatttt tggtgatgat caaaacgcgt
    15721 ttttctcgct ggctaacgtt ttttacgttc gccgctgccg tggcgctggc gctaccggca
    15781 aaagccaaca cctggccgct gccgccagcg ggcagtcgtc tggttggcga aaacaaattt
    15841 catgtggtgg aaaatgacgg tggttctctg gaagccatcg ccaaaaaata taacgtcggc
    15901 tttctcgctc tgttacaggc taaccctggc gttgatcctt acgtaccgcg cgcgggtagc
    15961 gtgttaacga tcccgttgca aaccctactt ccagatgcgc cgcgcgaagg cattgtgatc
    16021 aatattgcgg agctgcgtct ctattactac ccgccgggta aaaattcggt aaccgtgtat
    16081 ccaataggta ttggtcagtt aggtggtgac acgctgacac cgacaatggt gaccaccgtt
    16141 tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa acatccgcgc ccgttataaa
    16201 gcacagggaa ttgagttgcc tgcggtagtg ccggctggac cggataaccc aatgggccat
    16261 catgcgattc gtctggcggc ctatggcggc gtttatttgc ttcacggtac gaacgccgat
    16321 ttcggcattg gcatgcgggt aagttctggc tgtattcgtc tgcgggatga cgatatcaaa
    16381 acactcttta gccaggtcac cccaggcacc aaagtgaata tcatcaacac tccgataaaa
    16441 gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac atcagccgct gtcagagaag
    16501 attgatgacg atccgcagct gctgccaatt acgctgaata gcgcaatgca atcatttaaa
    16561 gatgcagcac aaactgacgc tgaagtgatg caacatgtga tggatgtccg ttccgggatg
    16621 ccggtggatg tccgccgtca tcaagtgagc ccacaaactc tgtaaatcct ggataaaaaa
    16681 aatccccgcg acacggcatg tcgcggggaa aaaatcagca gtagcaggca ttaatgaggg
    16741 ttaatgctta tttataaatt actgctgttc catggagggt attcggaccg gttacagaag
    16801 taatacggaa agattttgcg cccatctcat ccgctttttg cgccagctgc tcttccagcg
    16861 atcccagatt tgtccccgcg ttagcactga ttgtaccgac tttttgttgg ccttctggcg
    16921 ttgactgaac ttcgacagcc gcaaagctgg caaatgacat ggagcttaaa atcgccgcag
    16981 cgatgagggt ttttacgttt ttcataatag tggccttatg cagatgaatg acgtcgcaag
    17041 caattactta acgatcgata actaaatgat agatgtgatc tggatcacat acaagatatt
    17101 ttttataaca atcattaatt aatttaaaaa tctttaaatt tcagttaaat agaaagcaat
    17161 tatttttatg ggatctggac tggtgaacgg aggtgattat ttttataata acctttcatt
    17221 cacagtcacc aggtacaacg gcatcatggc aactgactca acacaatgtg taaaaaaaag
    17281 ccgtggccgc ccaaaagtgt tcgacaggga tgccgcgctt gataaggcca tgaaattgtt
    17341 ctggcaacac ggttatgaag cgacttctct tgcggacctc gtcgaagcga ccggagccaa
    17401 agcgcccacg ctatacgcgg aatttaccaa caaagagggg ttatttcgtg ccgttctcga
    17461 ccgctatatc gatcgttttg ccgctaagca tgaagcacag ctgttttgtg aagagaaaag
    17521 cgtggagtct gcgctggctg actattttgc tgccatcgcc aactgcttta ccagcaaaga
    17581 caccccggca ggctgcttca tgatcaacaa ctgcaccacc ctctccccag attcaggaga
    17641 tatcgccaat acgttgaaat cacgccatgc gatgcaagag cgcactttgc agcagttttt
    17701 atgtcaacga caagcgcgcg gggaaatccc gacccactgt gacgtgacac atctggcaga
    17761 attccttaat tgtattattc aggggatgtc gatcagcgca cgcgaaggtg catcgctgga
    17821 aaaactaatg cagattgccg gaacgacttt gcgtttatgg cccgaactgg tgaaataagt
    17881 cgcaaaaaaa gcccctcagc ctgagcgaat gaggggccaa attgcagagt acagccttgt
    17941 tatactttgt tattcaaaat cagttgccca ttactatcta gcgggatctg cgtacccgga
    18001 tcgcggtcca tgcggatttt gccctgctga tcgccaatct tataggtcac atcataaccg
    18061 agcatttttt ctgacttgtc atacaccgtt ttacaacgct gttgcgtagt cgtgtaagta
    18121 tcgctttcct ggagagagcc ctggatctgg ttaccggcat atccaccacc cagcgccccc
    18181 acaacagtgg cgacatcttt accgcgacca ccgccaaact gatgccctat cacgccgcca
    18241 gcaacagcgc cgagcaccga cccggtaatg cgattttcat cctgcaccgg tcgacgatgg
    18301 gtcactgtga cgttgcgaca ctcctgacgc ggtgttttaa ccgtttcctt gattggggtt
    18361 gcagaaacaa cctgagcgta ttgcgggcca cgttcaaaca cgttcagact ggccactgcc
    18421 gctacgccca gcgcagctgc gacaccaatc ccgatacccg ccaacattga tttattcacg
    18481 ggaacacctc cttctttgca gttatgccaa tcatgcaagc gaaggagaaa agcagctatc
    18541 agacagaaga tgaaaagcgc gggaatggct ggaaatactt aatgattcgg aggagtctga
    18601 aggaatgcct gatgcgacgc ttaacgcgtc ttatcaggcc taccgctcgt accatttgtc
    18661 cagggcggat gaggcgttta tgccacatcc gccagtgtac gtcgattaat gcaacttcaa
    18721 acgcggacgg ataacgcggt taatactccc caccagcatc attaatccgg ttttaaagta
    18781 accatgcagc gcaatctgat gcattcggta tagcgagata tatacaaagc gcgcaattcg
    18841 tccttcaatc atcattgagc cgcgcgtcag gttacccatc aggctaccaa cggtggagaa
    18901 gttcgacagc gataccagcg aaccatgatc tttatactga taatttttca gcggcttacc
    18961 attcatctgc gccagaatgt tgttcattgc gcaagtcgcc atctggtgtg cagcctgagc
    19021 acgcggcgga acaaagcccc cttccggacg cgggcatgac gcgcagtcgc caatagcgta
    19081 aatgtctgga tcgcgggtgg tttgcagcgt cggttccacc accagctggt tgatacggtt
    19141 agtttcaaga ccaccgatat ctttcaggaa gtctggcgct ttgatcccgg ctgcccacac
    19201 catcagatca gcctcaatat attcgccatc tttagtgtgc aggccgcctt catcagcact
    19261 ggtgaccatg gtttgcgtca gcacgcgaac gccaagtttc gttagctcgt tgtgggccgc
    19321 agcagagata cgtggcggta atgcaggcaa aatacgttct cccgcttcta ccagcgttac
    19381 gttcagggct tcgttggtca ggcctttgta accgtagctg tgcagttgct tgaccgcgtt
    19441 gtgcaattca gcggagagtt ctacacccgt cgcgccgccg ccgacaatcg caatgttcac
    19501 tttgccattt gcgcccaggt tggcggagta tttcaggaag agattcagca tctcctggtg
    19561 aaagcgacgc gcctggtgcg ggttatcgag gaaaatgcag ttctctttga cacctggcgt
    19621 attgaaatcg ttagaggtgc tacccagcgc cattaccagg gtgtcatagg cgattttacg
    19681 ttccggaacc agcagttcac ctttctcatc gcgcagttct gcaatagtga ttgttttcgc
    19741 ttcacgatca atatcaatga cggaacccag ctggaactgg aaaccatgat tgcgcgcatg
    19801 ggccagatag ctcaacgcat cgacgccttc atcaagcgag ccagtcgcca cttcgtgcag
    19861 cagcggtttc cacagatggc tgtggttacg atcgaccagc gtaattttgg cttttttctt
    19921 gcgtcccagc ttatgcccca gctgtgttgc catttccagc ccaccagcac cgccgccgac
    19981 aatcacaatc tttttcaatg gcgtagtcaa cgtgaccccc ttaaattt
//
LOCUS       DAZFJF010000085        19452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFJF010000085 DAZFJF010000000
VERSION     DAZFJF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.085"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            129..2339
                     /gene="katP"
                     /locus_tag="ACHKAU_003328"
     CDS             129..2339
                     /gene="katP"
                     /locus_tag="ACHKAU_003328"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO5532331.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            2383..2772
                     /locus_tag="ACHKAU_003329"
     CDS             2383..2772
                     /locus_tag="ACHKAU_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5532332.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            2886..3653
                     /locus_tag="ACHKAU_003330"
                     /pseudo
     CDS             2886..3653
                     /locus_tag="ACHKAU_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3166..3276
                     /locus_tag="ACHKAU_003330"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(3733..4128)
                     /locus_tag="ACHKAU_003331"
     CDS             complement(3733..4128)
                     /locus_tag="ACHKAU_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5532333.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            complement(4128..5087)
                     /locus_tag="ACHKAU_003332"
     CDS             complement(4128..5087)
                     /locus_tag="ACHKAU_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5532334.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5360..6262
                     /locus_tag="ACHKAU_003333"
     CDS             5360..6262
                     /locus_tag="ACHKAU_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5532335.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            6259..6570
                     /locus_tag="ACHKAU_003334"
     CDS             6259..6570
                     /locus_tag="ACHKAU_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532336.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            6646..7329
                     /locus_tag="ACHKAU_003335"
     CDS             6646..7329
                     /locus_tag="ACHKAU_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO5532337.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNRFEPEAA"
     gene            7329..7547
                     /locus_tag="ACHKAU_003336"
     CDS             7329..7547
                     /locus_tag="ACHKAU_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532338.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            7507..7993
                     /locus_tag="ACHKAU_003337"
                     /pseudo
     CDS             7507..7993
                     /locus_tag="ACHKAU_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8038..8277
                     /locus_tag="ACHKAU_003338"
                     /pseudo
     CDS             8038..8277
                     /locus_tag="ACHKAU_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKAU_003339"
                     /pseudo
     CDS             8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKAU_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            8517..9236
                     /locus_tag="ACHKAU_003340"
     CDS             8517..9236
                     /locus_tag="ACHKAU_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO5532339.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            9233..9830
                     /locus_tag="ACHKAU_003341"
                     /pseudo
     CDS             9233..9830
                     /locus_tag="ACHKAU_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10292..10792
                     /locus_tag="ACHKAU_003342"
     CDS             10292..10792
                     /locus_tag="ACHKAU_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO5532340.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(10794..11414)
                     /locus_tag="ACHKAU_003343"
     CDS             complement(10794..11414)
                     /locus_tag="ACHKAU_003343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532341.1"
                     /translation="MDLSDGAATATARGSCPGADAKVFDACGKKFCGWYIFPWTAAPC
                     CGRFSPRKVHSGKQAAGVAAEGLTAPSGAVALHTVRCAGSAVSGATGAARPERSLQAW
                     FFRHRTVAAARNRASGFGGDWRVRQQLPVGVGPTTGCRQAAGSRRVSPPPGIRTTETT
                     KQKNASHHDGQEGQRRRRGPPVFQAGVSLPGFSGGPAGHTPAGAGE"
     gene            11520..11954
                     /locus_tag="ACHKAU_003344"
     CDS             11520..11954
                     /locus_tag="ACHKAU_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO5532342.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            12047..12313
                     /locus_tag="ACHKAU_003345"
     CDS             12047..12313
                     /locus_tag="ACHKAU_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532343.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            12378..13268
                     /locus_tag="ACHKAU_003346"
     CDS             12378..13268
                     /locus_tag="ACHKAU_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5532344.1"
                     /translation="MSKKKNTTTPTPHDAAFWSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKNITH"
     gene            13268..13513
                     /locus_tag="ACHKAU_003347"
     CDS             13268..13513
                     /locus_tag="ACHKAU_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532345.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(13544..13795)
                     /locus_tag="ACHKAU_003348"
     CDS             complement(13544..13795)
                     /locus_tag="ACHKAU_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5532346.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(13829..14024)
                     /locus_tag="ACHKAU_003349"
                     /pseudo
     CDS             complement(13829..14024)
                     /locus_tag="ACHKAU_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14374..15222
                     /locus_tag="ACHKAU_003350"
     CDS             14374..15222
                     /locus_tag="ACHKAU_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5532347.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            complement(15308..15643)
                     /locus_tag="ACHKAU_003351"
     CDS             complement(15308..15643)
                     /locus_tag="ACHKAU_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO5532348.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            15875..16207
                     /gene="mobA"
                     /locus_tag="ACHKAU_003352"
     CDS             15875..16207
                     /gene="mobA"
                     /locus_tag="ACHKAU_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO5532349.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            16219..18939
                     /locus_tag="ACHKAU_003353"
     CDS             16219..18939
                     /locus_tag="ACHKAU_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO5532350.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQLMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM"
     gene            complement(19160..>19452)
                     /locus_tag="ACHKAU_003354"
     CDS             complement(19160..>19452)
                     /locus_tag="ACHKAU_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFO5532351.1"
                     /translation="QYDTDIESRANELWKKAVHTINNTTREERRVCYITLNRPEEEHS
                     DPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY"
BASE COUNT         4645 a         4660 c         5333 g         4814 t
ORIGIN      
        1 tgcggcttgg aaccccttag tattttttgt ctgtagtatc tatcccagca ataggtatat
       61 cctgttgcat caataaagtt gacttttgta tacaacatgc gaatttccct taatccggag
      121 ctattcgtat gataaaaaaa actcttcctg ttctgattct tctggcgcta tcggggagct
      181 tttctaccgc tgtagccgct gataaaaaag agactcaaaa tttctactat ccagaaacac
      241 tggatttaac tcctctgaga ttacacagcc ctgaatcaaa tccctggggg gctgattttg
      301 attatgccac cagatttcaa cagctggata tggaggctct gaaaaaagat atcaaagatt
      361 tgctgacaac ttcccaggat tggtggcctg cggattatgg tcattatggt cctttcttta
      421 ttcgtatggc ttggcacggt gccggaacat acaggacata tgatggccgg ggaggcgcca
      481 gtggtggtca gcaacgtttt gaaccgctga acagctggcc ggataacgtt aatctggata
      541 aagcccgtcg attgctgtgg ccagtcaaga aaaaatacgg ctccagtatt tcctggggag
      601 acctgatggt cctgactggt aatgttgccc ttgaatccat gggatttaaa acgctgggat
      661 ttgctggcgg aagagaagat gactgggagt cggacctggt atactggggg cctgacaaca
      721 agcctcttgc agataaccgg gataaaaacg ggaaacttca gaaacctctt gccgccacgc
      781 agatgggact tatttatgtc aatcctgaag gccccggtgg aaaaccagat cctctggctt
      841 ccgcgaaaga tatcagggaa gctttttcac gtatggccat ggatgatgag gagactgtgg
      901 ccctgatcgc gggagggcat acatttggta aagcacatgg tgcagcgtct cctgaaaaat
      961 gtattggcgc agggcctgat ggtgcacctg tggaggagca gggactggga tggaaaaata
     1021 aatgtggtac aggaaacggc aaatatacca tcaccagtgg cctggaagga gcctggtcga
     1081 catcgccaac ccagttcaca atgcagtatc tgaagaattt atataaatat gaatgggagc
     1141 tgcacaagag tcctgccggt gcttatcagt ggaagcctaa aaaagcggca aatatagttc
     1201 aggacgcgca tgatccgtct gtcctgcatc cgttgatgat gtttacgacg gatattgctc
     1261 ttaaagttga tcctgaatat aagaaaataa ccacccgttt cctgaatgat ccaaaagctt
     1321 ttgagcaggc attcgcaaga gcatggttta aactgaccca ccgggatatg ggaccggcag
     1381 cccgatatct tggtaatgaa gttcctgcag aatcatttat ctggcaggat cctcttcctg
     1441 cggcggatta tacaatgatt gatggtaaag acattaagtc gctgaaagag caggttatgg
     1501 atttgggtat ccctgcatct gagctgataa agacagcctg ggcttcagct tccacatttc
     1561 gtgtgactga ttatcgtggg ggaaataatg gtgcccgcat caggttacag cccgaaatta
     1621 actgggaagt taatgagcct gaaaaactga agaaagtact ggcatccctg acctcattac
     1681 agcgtgaatt taacaaaaaa cagtctgacg gaaagaaagt gtcgttggct gatttaattg
     1741 ttctttcggg taatgctgca atcgaagatg cggccagaaa agccggggtg gaacttgaga
     1801 ttccctttac tccgggaaga actgacgcct ctcaggagca gacggatgtt gcctcattca
     1861 gtgtactgga gccgacagca gatggattca gaaattatta ctcaaaaagc agaagtcata
     1921 tatcgccggt tgaaagcctc attgataaag ccagtcagct ggatctcacc gttcctgaaa
     1981 tgacggcatt actgggtggt ctgcgggtaa tggatattaa tacaaataat tcttcgttgg
     2041 gagtgtttac cgatacccct ggtgttctgg ataacaagtt ttttgttaat ctgctggata
     2101 tgtcaacacg atggagtaaa gcagataaag aagatacata caatggattc gatcgtaaaa
     2161 cgggagcatt aaaatggaaa gcatcctctg ttgatttaat cttcagttca aatcctgaat
     2221 tacgtgcggt ggcagaagta tatgcctcgg atgatgcgag aaataagttt attcatgatt
     2281 ttgttaaatc gtggaataaa gttatgaata gcgatcggtt tgatttaaac aataaatgat
     2341 aaaactgatg agggagggca cctccctcat gaaggagaaa ttatgcagaa aagcaataaa
     2401 gttataatca gctgtggtat ggcattatat tttttgtgtg gggggattac agtggcagaa
     2461 gaacttgatg aaagcatgca agttcttgca aggaatatga gtgtacttca gtcatcaatg
     2521 gataaaaagg agcttcttga agctcttgac agaatggatg atgctgtaga tgagtcaatg
     2581 aagcgtcttc ctgagagcat ttcaccggct gatgaagaag ggaagaatga ctatataaaa
     2641 gaacttcaaa agcttgaaaa agagattatg ctggcgaaac aaaaagtctt atcgggacag
     2701 ttatcagata tacccgattc ggtaaacaag atgaataaaa tcagagttga ggggcatgat
     2761 aaattcagat aaatcagttg gttgattcct gcgggacgaa tacatacgtc aggaacataa
     2821 ctttatggca gtgaaccgcc ccgggaatcc tggagactaa acttcctgag aaagaggtaa
     2881 acaggatgac taaaaatact cgtttttccc ccgaagtccg tcaacgggcg attcatatgg
     2941 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttgttcc attgcccaaa
     3001 gattggctgt acaccggaga ctctgcgtgt ctggggacgc cagcatgagc gggataccgg
     3061 gagcggtgat ggtgggctca ccagcgctga acgtcagcgt ctgaaagagc tggaacgtga
     3121 aaaccgtgaa ctgcgccgca gtaacgatat ccttcgccag gcttccgctt attttgcgaa
     3181 ggccgagttc gaccgcctct ggaaaaaata atgccactgc tgctgcgtga gcagtacggg
     3241 gtcggaccgg tatgcagtga actgcatatt gccccgtcaa cgtattacca ctgtcagcaa
     3301 cagcggtatc atccttataa acgtagtgcc tgtgcacagc gcgatgactg gctgaagaaa
     3361 gagatactgc gcttatacga tgagaatcat caggtatacg gtggcgtaaa gtctgacgtc
     3421 agttgttaca ggaaggtatc agggtggcca gatgtatagt ggcgcgcctc atggcggtta
     3481 tgggacttgc cggtgttctc cggggtaaaa aggtccgcac taccgtcagc cggaaagctg
     3541 ttgccgcagg cgacagcgta aaccgccagt tcgtggcaga acgtcctgac cagctgtggg
     3601 tggcggattt tacttacgtc agcacatggc agggcttcgt ctgtatggcg ttcgatttag
     3661 ggcactaaaa ccgctgctgg tccctcataa taaaagaaga gcaggtaata gacttaacct
     3721 gctcttcttt tattattttt ttaacataga aagattgcgt ctggtttgtt cttcgaaatc
     3781 atcttttgaa gtgatgctct ctgatttgtt ttctgaagat gcccctatga tgttttttaa
     3841 cacacttata tcaattgatg tagatgtgta tcctgttgtt tgctctataa gtttcaccag
     3901 attttctgca gtgattttct cactgaaaaa accactgatt agcgttaaca gtcgggggtc
     3961 aacagaataa agagctgctc cgcaaataag agattgtctg tagaagtctc cccgcatttg
     4021 tatcgggaga gagtctattg tttgttgtgt caggttatcc tgcggataaa gtgtcgggtg
     4081 caggtatgcc acaactttct ttcttttttt cggatcactg ctttccatta cgcatgcacc
     4141 attgctttca ccaatgcaaa ctgaggttca tccaccttgt tcactttcag attcgggaaa
     4201 tattgcttta tgtgagggta aataagctcg gctcctccgc cagtgagata gattcggttc
     4261 attcctgaga atgttgaaat ttcctgcctg atgctttctg ccagagattt cacttccctg
     4321 tctattacat gagtaatgtt ttttatttca gctacatcat ttattaatgt ctggagatat
     4381 tcattatcat gtctgttttt tattaactca tcagcaataa aataattaca tggtgtggag
     4441 gctttcgtca gcgcattcat gacggtatga gtgatgcggg atgtcccgat ttctgaatac
     4501 ccacggattt cagaaatgcc ttcaaatgca ccgagaatta aaccacaatc cagagtggtt
     4561 cctccaagat cgataatcag gctgcgttca agtttgttta ctttatcttt ttttagtgat
     4621 tcaaatgcag ctggcaaaga ttcaggcatt acattaactt ttttaatttt aaatgtttcc
     4681 cctttattca gagatatctc acggagaaca tttgcctttt ttctttctat tctttcttca
     4741 tttggttgat tgtcattatc aaagaattcg gttacaggta atgtcactgt cagttctaca
     4801 tcctgtggct gaaggccgga tgtcagcagt gcatgatgta tggcaagcaa attggtggtg
     4861 ctgtattgat aactgacatg tgttgtacct atgacagcag tactgcccaa atcataggtg
     4921 tatttttttc catccacgac gtaattgtaa actttgccgg cattaaacag tcccgggttc
     4981 cagccttctt taaagctgtt tcctgacaga ctggttctta cctcgccatc ttcaagccag
     5041 gccagtttta cgttagttga accatcatcg catgatactt ttagcatttt ttcttgcctc
     5101 gttttgagtt tttatttatc tcaaattgag catcctttat ctagatgcat ctttttgagt
     5161 tttttgttgt tccttattgt gtctcatttt gggttcatgt caagcgatga ggctctttta
     5221 gagtctcttt gcattcttgt tgtgactcag tttgagtctc tatcgatttc tcattttgcg
     5281 tttcgaactg agacggaaac tacttaacca ggccgcatgt gagaggagaa ttcccatgcc
     5341 aaactggtgc tcaaatcgta tgcatttttc tggtgaaccg gcacagattg ctgagattaa
     5401 acgactggcc agcggtgccg tcacaccgct ttatcgccgc gccacaaatg aaggtattca
     5461 gctgtttctg gccggaagtg ccggacttct gcagaccact gaagatgtgc ggtttgaacc
     5521 atgccccgga ctgacggctg ccgggcgtgg cgttgtatcg ccggagaata tcgcgttcac
     5581 ccgctggctg acatacctgc agaacggtgt actgctggat gagcaaaact gcctgatgct
     5641 gcatgaactc tggctgcaga gcggtactgg acagcgtcgc tgggaaggat taccggacga
     5701 tgtcagggat accatcaccg cacttttcac cgcaaaaaga ggtgactggt gtggcttctg
     5761 gagtaacgag gatgtatcgg tgtggtggaa ccgtctgtgt gacaacgtac tgccggaaaa
     5821 aaccatgccg tttgacctgc tgacggttct gccgacccgc ctggatgttg aagtgaatgg
     5881 ctttaacggt ggtgttctga acggtgttcc ttctgcatat cactggtata cggaacagta
     5941 tggcgtgaag tggcctgtgg ggtatgaggt gaatatcagt agtcagggag acaacttcat
     6001 tcaggttgat ttcgacacgc cgtggtatca gccggaaagc gacgttattg cagaattaag
     6061 ccgccgtttc agctgcacac tggagcactg gtatgccgaa cagggctgca atttctgtgg
     6121 ctggcagcga tatgagcgtg gcgagcttgt tgatgtgctg tggggggagc tggaatggtc
     6181 acatccgaca gatgatgacg agttgccgga agtcaccgga cctgcgtgga tagtcgacaa
     6241 ggtggcgcat tatggcggat gaagtatgac ggagacgggc gggcaactgc ccgtttcttt
     6301 tccggtaaag gatgtcgccg tgctcctctt tttactgcgc cggctgacgc ggcgcggcat
     6361 aaacgctgtc tgtggtcagt gtcccgcgtc cggcgggcac gggacgggcg cttttaccgc
     6421 tcccggctgg cgtcggtgac agtgtacgcc agcccgtcgc ccttttctga tgaacgccct
     6481 tcaagccgct ttcgcggcat agccttgccg tcaaaaagac gaaggctgcg gtattccacg
     6541 gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggt aacgggcacc
     6601 acaggggtgc cctcccggtg ccctctgtaa gagaaggagt tttgtatgtc ccgttttgtc
     6661 ctcggtaact gcatcgatgt tatggcccgt attcctgata acgccattga tttcatactg
     6721 actgacccgc cgtacctcgt cggtttccgt gaccgttccg ggcgcaccat tgccggtgac
     6781 gttaatgatg actggctgca acctgccagc aatgagatgt accgcgtact caaaaaagac
     6841 gcgctgatgg tcagcttcta cggctggcac cgtattgacc gctttatggc ggtctggata
     6901 cgcgccggat tcagcgtggc agggcacctc gtgttcacca aaaactacac atccaaatct
     6961 gcgtatgtcg cgtaccgcca cgaatgcgct tacatcctgg caaagggcag tccggcccgg
     7021 ccccgtaacc cgctgccgga tgtgctgggc tggaaatatt cgggcaaccg ccatcacccg
     7081 acggaaaagc ccgttaccag cctgcaaccg ctgattgaga gcttcacaca cccgaactca
     7141 attgtgctgg acccgtttgc aggcagcggt tcaacctgcg tggccgccct tcagtccgga
     7201 cgccggtata ttggtatcga gctgcttgaa cagtatcacc gtgccggaca gcaacgcctt
     7261 gccgccgtgc aacgggccat gcagcagggg gccgcaaatg ataacaggtt tgaaccggag
     7321 gcagcatgat gaattacagc ggatttgaag atatgcgtga acaggtggct gaactggcca
     7381 acaccatgtg cgacctgcgc acaaccatga atgagatgga gcagcggtac agcgatgcag
     7441 acaccctgcc ggaacgtctg gtgcgtcaga cgctctttcg tgccagccac ctgtttatga
     7501 aggcatataa cgaaatactg gaactggatt actgcttcaa agattgaggg gaaagagtat
     7561 gtacggaaca tgcgaaacgc tctgccgggc gctggcagca aagtatccgg gagacacgcc
     7621 gttgatgctg gttatctggt ccccggaaga gattcaggcc ctcgctgacg gaatggatat
     7681 ttccctggcc gatcatgaaa tcaggaccat cctggcgcgc ctggaggaca tcccggaaga
     7741 ccagcggact gagtccggta tttcttcctc cgcggcgatg gagattatca gcaacgtgag
     7801 cgaaaaccgc caggtgaccg tccctgctga actgctggcg tccctgattc agaccgctga
     7861 acaggcattg tggaagcgtg aatgggccgc ccgtgattac ggcctcgccg tcccggaatg
     7921 cgttacccgc cgtcaggcgg tggttaatca ggcccgcatc ctgctgaaaa acaacacgca
     7981 cgaaaacgac tgatgttatc gccgcctccg ggcggcgatt cagggagacc atgaatcatg
     8041 aataaaacgc tgaatgcact gatttgtcgt catgcccgcg acctgctgct ggcgcagggc
     8101 tggccggaag agacggatgt tgaccagcgt aacctgaagt atccgggctg gatcagcatt
     8161 tacgtgcggc tggatgcgcc ccggctggcg acgttactcg ttaaccgcca cgatggcgta
     8221 ctgccgccgc acctggcctc cgccattcag aaactgaccg gaaccggggc ggaacttggt
     8281 ttcacgccag cccacgaacg tttccacctg gcattatgtt cgccgggaga cgtctctcag
     8341 ctctggatgc tggttctggt gaattgtggt ggacagcctt tcgccgtcgt tcaggtgcaa
     8401 catatcttca cgcctgtcgc tatcagtcac acgctggcgc ttgccgcgac acttgatgcg
     8461 cagggataca gtgttaacga catcatccat atcctgatgg cagaaggagg tcaggcatga
     8521 gcgcacgttc acgggcactg attcccctca gcgcagaaca acaggccgcc atgcaggctg
     8581 tggctgtcac agaacaacgt cgtcgtcagg gacgcacact ttcagcatgg ccttatgcca
     8641 gcgctttctt tcgctgcctg aatggcagtc gccggatttc gctgaccgat ctccgctttt
     8701 ttgcccctgc gctgacgaag gaggaatttc atggcaaccg tctcctgtgg ctggctgccg
     8761 tggataaact gattgaaagt tttggtgaag tctgtgttct tcccctgcca tccgatgcgg
     8821 ggcatcgtct gttcccgtcc gttccttttc gtgaaggtga gcgtcgtcgt cagaaaacca
     8881 cgctgacaga gcagaaatac agccgccagc gggaacgtga ggcagaacga cgggaactgg
     8941 aataccagac atgttttgct caggcgcaga ttgaccttgc gtttcatact cccgccacgg
     9001 tcggaagctg gttgtcccgc tggtctggtg ttgttgagga gcatgatctg gaaacgattt
     9061 tctgggggtg gtgcgggcgt tttccatcac tgtcatcatt tgaccggttt ttctggcagg
     9121 aggaaccact ctggcggctg atttttgaag ccggtgaggc cggtcgtggt gcaccggtac
     9181 aggtacgtgc acttgagcag tggatgatcc cgaacaagct ggagaacgca atatgatgaa
     9241 atcagacgaa caatatcagg ttcccgtgtg gatgcgacct ctgttgccgt tgctctgcaa
     9301 taccggtggt aatgatccgg aggaacttct gaatgataca gaaaccaccg ccagtgcgaa
     9361 tattgtccgt tatgtactga tagttgcagt gcggtcgcag attggatctg ctgcagctac
     9421 tgtacaggaa aggactgttg cgcacagaga taccaggtgg cttttcaccg gaagaagcgc
     9481 aggaactcct ggataatctg gtgcgcagcc atatcagcaa ggcgctgtcg ggtgagcgaa
     9541 tggcagcccg tgacagatat gccgagctgg cctggattcg ccagcaactg gtggatgccg
     9601 cctggtttgt ccgtgccaca ctggaagcgc atggtacgag cgtcggaaat gagagtccct
     9661 ctgcacttcc agagacaatg ccggacatac agacacggga actggtgatg ctgatcaaac
     9721 gactggcatc ttctctgaaa gcggtgaaac cggacagtag cgtggtgcgt gcggcgcagg
     9781 actggctgtg cgacagaaaa cttgtggata tcacagatat tctccggtga accaggcatg
     9841 tatatttttc gcagggacac gccgatcatg atcgtttcat atcaggggtg gtctggaggt
     9901 catgcggcgt gtcctctgca ctcgccggaa taaggaagtc gccggcggat ccgcttttac
     9961 ccggccatgc ggggcatggc cttgtgggtt ttcagttatg tggcctcagc gtcgtgtgcg
    10021 ggctgtgccg tgcctccatc gtagccgggc ttctgcgggc gggatgcaag gtaagatgaa
    10081 ccgctgcggt cacccggtcc ctccttcccg tctgccgtga ttttccggtc gttcacccgc
    10141 tcagatttcg cggtctcacc ctccaactcc cgtcagccgt gcgcgtcggg ctgcggcttc
    10201 ccttgcgccc ttgcatccct gcaggcccgg caaagatgga ggcacggcac cgcccggtgt
    10261 cgcagaacat gtgactatgg aggattcggg aatgtctgtt gttgcacctg ctgtatacgt
    10321 tggaacctgg cataaataca actgtggaag catcgctggc cgctggtttg acctgaccac
    10381 gtttgatgat gaacgcgact ttttcgccgc ctgccgtgct cttcaccagg atgaagccga
    10441 tcctgaactg atgtttcagg attatgaggg gttcccggtg aatatggcct ccgaatgcca
    10501 tatcaactgg gcctgggttg aaggcttccg ccaggcacgg gatgaaggct gcgaagaggc
    10561 ttatcgtctc tgggtggatg ataccggtga gacggatttt gacaccttcc gcgatgcctg
    10621 gtggggcgag gctgacagtg aggaggcttt tgcggttgag ttcgtcagtg ataccggcct
    10681 gctggctgac gtgccggaga cggtggcgct ctattttgac tatgaggcgt atgcgcggga
    10741 tttattcctg gactccttca cctttattga cggtcatgtg ttccgtcggt gatttactcc
    10801 cccgctccgg cgggggtatg gcctgccggg ccgccggaaa acccaggcag gcttacgcct
    10861 gcctgaaata caggagggcc gcgcctgcgg cgctgccctt cctgcccgtc atggtgtgag
    10921 gcatttttct gttttgtcgt ttctgttgtt ctgatgcccg gaggcgggga cacccttcgg
    10981 ctcccggctg cctggcggca tccggtcgtc ggcccgactc cgacaggcag ctgctggcgc
    11041 acccgccagt ctccgccgaa cccgctggcg cggtttcggg ctgccgctac ggtccgatgt
    11101 ctgaaaaacc aggcctgcag cgaccgttcc ggtcgcgccg cccctgtcgc gccggaaacg
    11161 gcgctccctg cacacctgac ggtgtgtaag gccactgcgc ctgatggcgc agtcaaccct
    11221 tctgccgcca cccctgcggc ctgtttcccg ctgtggactt tccgggggct gaagcggcca
    11281 caacacgggg cagccgtcca ggggaagatg taccagccac aaaacttttt gccgcaggcg
    11341 tcaaaaactt ttgcgtctgc ccccggacaa ctgccccgtg ccgtggcagt cgctgcccca
    11401 tcggaaaggt ccactgcggg gagaaacccc gaagaagtgg acggcagaag gggctttcgc
    11461 ccctccgggg gagcaaccgg tcggcccggc gacctgattc agcagaagga gacgttttca
    11521 tgacagtcag cagcacgatt tccgtttttt gccgcgacgg ggtgttccgt accgtttact
    11581 gccacctgca cggggagccg acctggaacg gtcgcatcct gcatacccac tatgccaccg
    11641 gtcagctggc cgaagccctg gttgaacacg gagatatccg ttgcctcggt ccccgttgcg
    11701 acaaatccgc cgggcatacg cttcagaacc cggtggacgg tgtgacggct tattacggac
    11761 gtgacagtgg tttccggatg gacagtgagg cgcgtgagta ccgttctttc atggaggcta
    11821 ttgccactga aagcactgaa gaggtgcgct tccattatgt gttcatcgac ggctactgga
    11881 aggtgatgta ccgcacgccg gaaggctgga agatgaaagc gctcgcgctg gcactgcgcc
    11941 gctgtccgaa atgaaaaaag gcagggcgat aaaccctgcc tttctccgcc ggcgcttccc
    12001 cgtacggaag tccggcatct caatcactat ctatggagat tatgccatga gaccatccat
    12061 tatcttcgca accgccgagt atgtaaagcg tctgcgtgaa gagtgcctgc gggagaataa
    12121 acccctgcac cgccataccc gcgtcagacg tcaggagctg gcacaggatg agattaaccc
    12181 ggacgtcctg gcgatgagcg gtcatatcgc cagacgctgc agtgagcaga agcgggtacg
    12241 tatccccgcc atgaaagtca gcgaatgggg ccacctgctc cgcgcgcttg aaattgagcg
    12301 ggcctgccac tgattaaccc ttgccgtctg cctgtgcagg cggcaggata cgttcagaca
    12361 tctcaccagg aggaaccgtg agtaagaaga aaaacaccac aacgcccacg ccgcatgatg
    12421 ccgcgttctg gtcgttcctg gcgaatcccg acgtcgccag agattttctg gaactgcatc
    12481 ttccggcgga gtaccggcag ttgtgcgacc tgtccacgct gaagctggaa cccgccacct
    12541 ttgttgagcc ggacctgcat cagtacgcca gcgatatcct ctggagtgtg aaaaccaccg
    12601 ggggtgaaga cggctatgtt tatacgctca tcgagcacca gagcaccgaa aatctgtaca
    12661 tgcctttccg catgttacgt tacagtgtgg cggcgatgca gagacatctg gagcagcaca
    12721 aaacattgcc actggtgatt ccggtgctgt tctatcatgg tgagcgcagc ccgtacccgt
    12781 acagcatgaa ctggctggac tgttttgagg aaccggcgct tgcggctaaa atatacacaa
    12841 agccgtttcc gctggttgat atcactgtcg ttgatgacaa tgaaatcatg aaccatcgcc
    12901 ggatggccgc actgacgctg ctgatgaagc atatccgcca tcgtgacatg atggagctgc
    12961 tggacaaact cccgcaggtc atggtggaaa tttcagacga gcaggtgcgt gttctgattc
    13021 attacatcgt taacgcaggg gactctgtat caccggaatt tatgcgggcg ctggctgagc
    13081 gtctgccgca gcatgaggat aaactgatga ctatcgctga acgtcttgag caaaaaggtc
    13141 gccaggaagg agcgcttgaa aaagccctgg ctattgcgtg ccagcttcag aaaatgggga
    13201 tgacgccgga gcagattaag caggctaccg gactttccga agctgaactg aagaacatca
    13261 ctcactgatg agaaccacaa agcccggcaa aatgccgggt ttttttgtca gcaggagcag
    13321 agcaaacagc atgacgattg cagaacgtct tatacagaaa ggtttcgacg aaggttttaa
    13381 gaaaggtgcg cttgaagtgg cgcgggaagc agcctgccgg ctgcgggata tgggctggac
    13441 gccggaacgg attcaggagg caaccggact ttccggtgaa gaactgaaaa agctgtttcc
    13501 tgatgagcag tagcctggca ttctgccagg ctgtgaatca cgctcacaca ttccctgccg
    13561 tttcccccag ttcattttcc agacgcctga tgatatctgc cttgcgcatc ccctgcatgt
    13621 gaggcagcag atcacggact gaaagtgcct gggtggtcct ccagacggtg acatccacct
    13681 gttccgcatt ttcccaggcg tgccatgttc tctgtgtcac gccaaattct ctggccgctt
    13741 tttcgcgtga ccagagcatg ctttttcgcc agattcgcag ttcccagccg gtcataaaac
    13801 acctttgaaa aagtgaaaaa acttcacttt attttatcat cataatcacc aggtcagcgc
    13861 gataaataag agtgaaaaag tgaagaaatt tcacttttta gttgtaggtg tcgtgctgtt
    13921 ttctctgttt tcacctgtgc gggagtggtg cgccagtgac caccacatgc cattgttgtg
    13981 ctgtcgtttt tcttcagtcg cgccggatgc ggcgctccct gcacacctga cggtgtgtaa
    14041 ggccactgcg cctgatggcg cagctgtctc tgcctgtctg ccgccgcact gcggcccgtt
    14101 ctccgcagac ggactttccg ggggtacata cacgaccaca tcccgtttca gtcgtccagg
    14161 ggagagatgt accagccaca aaactttttg ccgcaggcgt caaaaacttt tgcgtctgcc
    14221 cccggacaac tgccccgtga cgtggtgagc gtcccccatc ggaaaggtcc actgcgggaa
    14281 gaaccccctg tgaagtggac ggcagaagga gcttcgctcc tccgggaaat gaccaggctg
    14341 acccggcagc cgtgacgatg aggagacaat atgatgaatc agactttacc cactgctgac
    14401 ctgaatactg ccggcacgac agatattatt ccgtctatgg ctatcgaccg catcatcgcg
    14461 cagcgtaacg aaggtattgc actgttcatg caggcgatgg aatgcctggc gacagcgcgc
    14521 aggattctgc tcgatgcttc cggtgacatt tttctctacg ggtttgaaga ctgcgtgact
    14581 gactccgttc gatgcatgga taaaccggaa gaagcaaaaa agaatatcac ccgtcttgcc
    14641 gaccggaaaa tctgggatcg cctgatgact tatacgggca tgtacacctt catgagttca
    14701 tgccagcgtg atgagtggaa cagccagctg atgagcgaca cctgtcctga aatcaccctg
    14761 gacaatgtac tggcaacatt ccgccatctg aatgccagta agatgcagac atttgaacag
    14821 gaactgattg atatctggcg gaaattgtca tgggattaca gaaccaataa tccctgccgt
    14881 ctgggcaaga gaatcattat tgaaaacctg ctgtaccgct ggagtaacgg gcgtgtgacg
    14941 ctggactgca gcggacggga ggcactggat gacctggtac gtccgtttta tctgctggag
    15001 ggacgcaacg ttcctgactt caggaacagc atcggggcgc agtatggtga atttctcggg
    15061 aacggcgaca atatcggtga gttgtttgaa ggggaatatt ttacggtgcg tggctaccag
    15121 aaagggaccg tacacattgt ctttaagcgt cctgaccttg ttgaaaaact gaatgatatt
    15181 attgcacggc attatcctgg tgtattgccg ccacgagtct gattaaacag aaaagccctg
    15241 gtatttatgc cggggctttt ttttctgtaa tcatcataat ctcagaatac gatgtgacat
    15301 tgtcacatca attgttgttc atatcgatgc catctttcct gcctgaatct gtctttttag
    15361 gtgctgtttt agaggaagaa cggcgggatg gttttttcga ttctgcttcc cgaataatgt
    15421 cacgaagtgc cttctctcct acttcatacc ctgcattttt gagcgtgtca cgaacatctg
    15481 ccagtgtata accctttgtc cttaccagga caatgatgtc atcacgaatg gcatcaagaa
    15541 aatcacgcaa tgtttttcgt tgagcggtga ggtccggcaa ttctgataaa gcagcttttg
    15601 ccagctggat gtcatcatct gagtaaaatt ttttagaagc cattggcact ttttctccgt
    15661 ttcatgttga agtctgattt tatcatcaaa aactgcctcc tggcagagct gcgccccgcc
    15721 tctgccgtct gcataagact atgatgcaca aaaataacag gctataatgc ctgaaaaacg
    15781 ggacaggatg tgtaatcgta taaccgcgca cgcacggcta tacgattacc acctggtcag
    15841 ggcttagccc cgacaccccg gtaaggagca gaccatgagt gagaagaaaa cccgaagtgg
    15901 cagtgagaaa cggcaaaaga atgtcctcat tgctgttcgt ttttcaccag aagaggctga
    15961 gattgttaaa gaaaaggcag aaaaaaacgg actgacagtt tcaactctga taagaaaaac
    16021 tgtgctggga aaacagataa atgcccgcat tgacgaagat tttttgaaag agctgatgcg
    16081 tcttggtcgc ctgcagaaac atctgtttgt ggaaggaaaa cgcacgggag ataaagagta
    16141 tgcagaggtt ctcgttgcta ttacagagct tgctaatact ctgcgaagag atttaatggg
    16201 acgctgagat aatcgctgat gaatgccatc attccgaaaa aaagacgcga cggaaagtct
    16261 tcttttgaag acctgattgc gtacacctca gtgcgggatg atgtgccgga agatgaactc
    16321 aggccagaca cggaaatgaa agctgagatt ccgcaccgga accgcttcag acgtcttgtt
    16381 gattatgcaa cacgtctgcg taatgaaaaa tttgtttccc tgattgatgt gatgaaagac
    16441 ggcagtcagt gggtgaattt ctatggtgtg acctgctttc ataactgttt gtctcttgaa
    16501 tctgcggctg aagaaatgca gaaagcagca gaccttgctc atttttccaa agatgataca
    16561 gatcctgttt ttcactatat actctcctgg cctgcgcacg aaagtccgcg ttctgagcag
    16621 ctttttgact gtgtgcgtca caccctgaaa tcccttgagc tgtcaaaaca tcagtatgtg
    16681 gcagctgttc atacagacac ggataacctg catgtacatg ttgcagtgaa tcgtgtccac
    16741 cctgaaaccg gctatatcaa ttgccttccg tggagccagg aaaaactcag tcgtgcatgc
    16801 cgtgaacttg aaattaagca cggattcgcg ccggataatg gctgctttgt gcatgcaccg
    16861 ggcaatcgta ttgtgcgcaa aacagcgctg gtacgtgagc gccgtaatgc ctggcgacgc
    16921 gggaaaaagc agacgttcag ggaatatatt gttcagatgt cgattgccgg gctgcgtgaa
    16981 gagcccgcac aggactggtt atctttgcat aaacggctgg ccagtgatgg tctgtatatc
    17041 acgatgcagg aaggggaact ggtggtgaag gatggctggg acagagcgcg ggaaggtgtt
    17101 gcactcagtt cgttcggacc gttatggaca gctgaaaaac tggggcggaa actgggggaa
    17161 tatcagcctg taccgacaga tattttcagt caggtgggga cacccggtcg ttatgatccg
    17221 gaagccataa acgttgatat ccggccggaa aaagtggcag aaacggagag tctgaaacag
    17281 tacgcctgcc gtcattttgc cgaacgctta ccggaaatgg cgagaaatgg tgagctggaa
    17341 agttgcctgg atgtgcacag aactctggca acggctggat tatggatggg gatccagcac
    17401 gggcatctgg tactgcatga tggatttgat aaacaacaga caccagtacg tgcagacagt
    17461 gtgtggccgt taatgacgct ggattatatg caggatctgg atggtggatg gcagcctgta
    17521 ccgaaggata tttttactca ggttataccc ggagaacggt tccggggacg taatcttggt
    17581 actcaggctg tcagtgatta cgaatggtat cgtatgcgga tggggacagg tccacaggga
    17641 gcaataaagc gcgaactgtt ttctgataag gaaagtctgt ggggatacgg ccttattcag
    17701 tgcaggagcc tgattgaaga gatgatagct gacggggatt tcagctggca ggcatgtcat
    17761 gagatgtttg cgcgtaaagg gcttatgctg cagaaacagc atcatggtct tgtgattgtg
    17821 gatgcgttta atcatgagct gacaccggtg aaggccagca gtatccatcc tgatttgacg
    17881 ttatcccgtg ccgagcctca ggccgggcca tttgaaattg cggctgcaga tattttcgaa
    17941 cgggtgaagc ctgaatgtcg ttacaatccg gaacttgctg ccagtgatga agtggaacca
    18001 ggcttccggc gcgatcctga gattcgtcgt gagcgccgtg aagcccgtgc agctgcgcgg
    18061 gaagatttgc gtgcgcgtta tcttgcatgg aaggagcact ggcgtaaacc cgacttacgt
    18121 tacggagaac ggttacggga aattcatgct gcctgccgtc ggcgcaaggc gtatatccgt
    18181 gtgcagttcc gggatccaca attgcgtaag ctgcattacc atattgcgga agtgcagctt
    18241 atgcaggcgc tgatcaggct gaaggagagt gtgaaggaag agcggttgtc actgattgcg
    18301 gagggtaagt ggtatccttt gtcctaccgg cagtgggtgg aacaacaggc ggctcaggga
    18361 gacagggctg cagtatcgca gttacgtggc tgggattatc gtgatcgtcg ctcgcgtaat
    18421 aaggataaaa gacgcacaac taatgttgat cgttgtgtcg tactctgcga acccggagga
    18481 acgccacttt ttaataatgt tgcaaaactt gaagctcgtc ttcagaaaaa tggaagtgtt
    18541 catttcaggg atacgcggac aggaaaaaat gtctgtacgg attacggaga tcgggttgtg
    18601 ttctaccatc acacggaccg aaatgaactg gcagagaagc ttgatttaat tgcgccagtt
    18661 ctgttcagcc gcaacggaaa gcttggtttt gagccggaag gttcatacca gcagtttaat
    18721 gatgtgtttg cagaaatggt ggcctggcac aatgcagcgg gaataaccgg aaacggacac
    18781 ttcaccatta ctcgtccgga cgtggatttg caccgtcaac gcagtgagca gtattaccgg
    18841 gaatatatca gacagcagac ccgtttatcg gtgagccatg atgataatta tacgttacgg
    18901 caggagaaaa catgggagcc accatcacct ggaatgtaag cccggataag tgatggtgca
    18961 aagattcagc ccaaaaacgg caggaacctt cgtcaataag ggcgggagta gtcactgaat
    19021 tgagtttata cccggttaac tctgaattaa gggccgtgtc tgcggccctt atccagtgcg
    19081 ccgtaaaacc ccggacttag tgaacagggt tttacagtct gattgacaga tatggtcacc
    19141 agaaaatatt tgtattcctt cagtattttt cgtcagttgt tggttgtgct ccacatgatt
    19201 tctggggatg atcattattt attgtctgcg atttattatt ttcagcgacc ggtaacagta
    19261 tctccagggg gagcagggta ttcagaatcg ccttcactga aggtatatct tcaggatcag
    19321 agtgttcctc ttccggacga ttcagtgtga tataacatac ccggcgttct tccctggttg
    19381 tgttattgat agtgtgcaca gcctttttcc acagttcgtt tgcccgggat tcgatatctg
    19441 tatcatattg cg
//
LOCUS       DAZFJF010000086        19428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFJF010000086 DAZFJF010000000
VERSION     DAZFJF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.086"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(138..1007)
                     /locus_tag="ACHKAU_003355"
     CDS             complement(138..1007)
                     /locus_tag="ACHKAU_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO5532352.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="ACHKAU_003356"
     CDS             complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="ACHKAU_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5532353.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2467..3033
                     /gene="efp"
                     /locus_tag="ACHKAU_003357"
     CDS             2467..3033
                     /gene="efp"
                     /locus_tag="ACHKAU_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5532354.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3085..3210
                     /gene="ecnA"
                     /locus_tag="ACHKAU_003358"
     CDS             3085..3210
                     /gene="ecnA"
                     /locus_tag="ACHKAU_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5532355.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            3321..3467
                     /gene="ecnB"
                     /locus_tag="ACHKAU_003359"
     CDS             3321..3467
                     /gene="ecnB"
                     /locus_tag="ACHKAU_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5532356.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            3649..3966
                     /gene="sugE"
                     /locus_tag="ACHKAU_003360"
     CDS             3649..3966
                     /gene="sugE"
                     /locus_tag="ACHKAU_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5532357.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3963..4496)
                     /gene="blc"
                     /locus_tag="ACHKAU_003361"
     CDS             complement(3963..4496)
                     /gene="blc"
                     /locus_tag="ACHKAU_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5532358.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4585..5718)
                     /locus_tag="ACHKAU_003362"
     CDS             complement(4585..5718)
                     /locus_tag="ACHKAU_003362"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5532359.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="ACHKAU_003363"
     CDS             complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="ACHKAU_003363"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5532360.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="ACHKAU_003364"
     CDS             complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="ACHKAU_003364"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5532361.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="ACHKAU_003365"
     CDS             complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="ACHKAU_003365"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5532362.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="ACHKAU_003366"
     CDS             complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="ACHKAU_003366"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5532363.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATELAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            9417..10394
                     /gene="epmA"
                     /locus_tag="ACHKAU_003367"
     CDS             9417..10394
                     /gene="epmA"
                     /locus_tag="ACHKAU_003367"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5532364.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10613..12115
                     /gene="yjeM"
                     /locus_tag="ACHKAU_003368"
     CDS             10613..12115
                     /gene="yjeM"
                     /locus_tag="ACHKAU_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5532365.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            12167..12481
                     /gene="yjeN"
                     /locus_tag="ACHKAU_003369"
     CDS             12167..12481
                     /gene="yjeN"
                     /locus_tag="ACHKAU_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO5532366.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            12478..12792
                     /locus_tag="ACHKAU_003370"
     CDS             12478..12792
                     /locus_tag="ACHKAU_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO5532367.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(12821..16144)
                     /gene="mscM"
                     /locus_tag="ACHKAU_003371"
     CDS             complement(12821..16144)
                     /gene="mscM"
                     /locus_tag="ACHKAU_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5532368.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(16166..17134)
                     /gene="asd"
                     /locus_tag="ACHKAU_003372"
     CDS             complement(16166..17134)
                     /gene="asd"
                     /locus_tag="ACHKAU_003372"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5532369.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(17231..18283)
                     /gene="rsgA"
                     /locus_tag="ACHKAU_003373"
     CDS             complement(17231..18283)
                     /gene="rsgA"
                     /locus_tag="ACHKAU_003373"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5532370.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            18378..18923
                     /gene="orn"
                     /locus_tag="ACHKAU_003374"
     CDS             18378..18923
                     /gene="orn"
                     /locus_tag="ACHKAU_003374"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5532371.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            19134..19209
                     /locus_tag="ACHKAU_003375"
     tRNA            19134..19209
                     /locus_tag="ACHKAU_003375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19167..19169,aa:Gly,seq:gcc)
BASE COUNT         4502 a         4950 c         5090 g         4886 t
ORIGIN      
        1 caggcattgc aactgattga atgcttgaga atttgtaggc cggataaggc gtttacgccg
       61 catccggcat gaccaacgat cacgttatca gtcatttcag gctggatggc tccagcctcg
      121 cattagcaaa gatgagatta tttcgcctgt ggtgcagttt tgctggtgga ttgaacacgt
      181 aagtgatgca gcgcgcgtaa gcattgatcc tgggttagcg gttgggcgtt attcgccgct
      241 acggtgcgaa catagacctg acatggcaca cccgcgagtt ttttcagtcg ttggctgaaa
      301 tcaagcattc ggcgagtgat cgcctctatt tctttacccg gcgttgcttc acgcgttttc
      361 accactgcgc cgctaaattg ttctttgcca ctttcatctt taaagcggta aagcaccgcc
      421 agataatggt cgaacaggtt atgtttccat tccggttggg aataggtgtc atattccaga
      481 ttgccatttt cctggcagtc aacgtcatat aacggcagga aatctaacag tgaggtaata
      541 cgtaatgcca gttcacgcat ttccgcatta ttaattgcat gaatgattgc tcgcgccagc
      601 atttcaatct gcaactcatt gacgactaac tcacatttgc taccgtcatg tagcgttaat
      661 acgaaggtaa gattttcgcc ttgattatcg gtaagttcca gcgtgttaac ccgacgatta
      721 atatcggcat ttttaagctc atcaaccaga atttctggga tgttttctgc cattttctta
      781 atgactttat cgcgagcttg ctcatactga aggcgtgcag cagcatccag tttatgcttt
      841 tgatgcatac gactttccag cgcaatcagc aggtcgcgca gttccatgac agacataaag
      901 aacaacgatt ctttattgcg aggttcttta attttcagcg ccagtgcaat gaaattatta
      961 ctcttgcgaa taacaccggt attaacaccc ttgatgctta tggccatgta ggttctccct
     1021 aaccatttct caattaaata attaatttta atttataagc cagataaatg ggcttggtag
     1081 taatagttgt taaaataaca taaatagccg tactcactct atataaacag aaaagaaagg
     1141 ttaattattg gtgttggcta tataaagagt aacaacagca atgtatatga ataattaaag
     1201 gttatgcaac gggcaaagat taaatttccc agtttaatga caggttgctg tacttgcgtt
     1261 tgttgctctt gccggatgcg gcgtaaacgc cttatccggc ctacagttca ttgcaaatcc
     1321 attcaatttc aatgcacaat gtaggcctga taagcgtagc gcatcaggct aatgtttaac
     1381 ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt ttgctgggtt
     1441 cgccgccgat ttctcgcgcc agtttcggca ccagataacc cgacaccagt gtcagcaact
     1501 cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg ccctgtactt
     1561 tatcgagcac atgcaggtaa tacggcatta cgccggcatc gaacaacgca ttactcaggt
     1621 ttgccagcgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg ttaagcaggg
     1681 tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct acctcattgg
     1741 catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag cgttcaacca
     1801 gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc cgcagacgtt
     1861 tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga tctttcgcca
     1921 tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca gcaacatact
     1981 caagagcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg aagtggcgac
     2041 ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc gcccggttgt
     2101 ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagcggg tcggtggaga
     2161 atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa agaggatcgt
     2221 ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg gcaaacagct
     2281 ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa agacgcagaa
     2341 gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct ctggatgggg
     2401 tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca aatttcagag
     2461 ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat catgttagac
     2521 ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg ccaggcattt
     2581 gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac cttcaaatct
     2641 actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct gtacaacgac
     2701 ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc tgatgcaaaa
     2761 gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt aactctgtgg
     2821 aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat cgttgatacc
     2881 gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac cctgtctact
     2941 ggcgctgtgg ttaaggttcc gctgttcgta caaatcggcg aagtcatcaa agtggatacc
     3001 cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc ctgcaggctg
     3061 caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt tttgcttgcc
     3121 agcacgctgc tcacgggctg taacaccgct cgcggtttcg gcgaagacat caaacatctc
     3181 ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa atcatcagat
     3241 ttccgtcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac ataaataaca
     3301 ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt tctgttctgg
     3361 tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa gacatttctg
     3421 atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaatagcaa taagcaataa
     3481 cggtacgaca ggtatgtcgt gccgtttgtt ttttctgcga tagtcactaa ggtaatagtt
     3541 gaaattcccc tgccacctgg caaaatatcc gttcaaccat cagctttgca ggacgacctg
     3601 caaacgcctc ttttcaccgg ggacggcccc aattctccgg agcctgatat gtcctggatt
     3661 atcttagtta ttgctggtct gctggaagtg gtatgggccg ttggcctgaa atatacccac
     3721 ggctttagtc gtttgacgcc gagtgttatt accgtgacgg cgatgattgt cagtatggcg
     3781 ctacttgcct gggcgatgaa atcgttacca gtagggacgg cttatgccgt gtggacgggt
     3841 attggcgcag tcggcgcggc catcaccggc attgtgctgc tcggtgagtc cgctaacccg
     3901 atgcgcctgg cgagtctggc gttaatcgta ttggggatta ttggtctgaa actcagcact
     3961 cactaactac cgggctgctg tacccaaata aacttactga catcaaaccc ttcccgggtc
     4021 gcgactgcca gcatctcctg tttcacttcg tcagaaatgg ttggcgtgcg ggagagtatc
     4081 cacaggtagt cgcggtccgg gccgcaaacc agcgcatggc ggtattcccg atcgagtgca
     4141 ataacgttat aaccgccata gaaaggacca aagaatgaca ctttcagcgc agcgcgagtt
     4201 ggtgcgccgg taaagtacgc tttcccttca ctctgctgcc acattcctct gtcagggtta
     4261 tagcctttat taatgacatt caggccgccg tcatcacgca ggctgtatgt tgcggtgact
     4321 ttttccagtc cacgttcaaa acggtgatca aaacgggcaa tctcatacca ggtaccgaga
     4381 taacgtttgg cgtcgaaatt attcactact gttacgccac gcggcggcgt aggagaactg
     4441 caggcaacga ccagaaatgc agctgtcgct gcggcaacga gagggagcag gcgcataaat
     4501 gtttccttac tggttttttt ctaagtgtag atgacagcaa ggaaaagcgc agaaaaggtc
     4561 cgaaaattcg gacccgatga aattttactg tagagcgttg agaatctgcc aggcggcggc
     4621 gactctcgct ggattgggat agtttttgtt agccagcatc actataccca gctctttttc
     4681 tggaataaac gcgacatagc taccaaatcc gccggttgcc cctgttttat gtacccatga
     4741 tgcgcgtact gcaggagttg ggggcgtaat cgcttttacg gggtgtgctg ccagtgcaat
     4801 tttattgcca ctgccgttaa tgatgctgtc aggatttacc ggccagtcca gcatttccca
     4861 gcccaggccc tgatacatat cgccggtttg ccagtagcga gattgtgcca gttgtatccc
     4921 ttgctgaagt gttttgtcgt tgatatcacg gggattcata ttgcttcgta cccagcaggc
     4981 catatcttca atggtcgact tcacaccata agtttcagca tctaacgccc ctggcgaaac
     5041 atgaactgcc ttgccttcgc gatatcccca ggcgtaattc ttttcttctg cgggcggtac
     5101 attaatccac gtatggttga gtttgagtgg ctggaagaca cgtgtttgca tcgcctgctc
     5161 aaaactcaaa ccagacggct tcacagccag tgcgccgaac aaaccgatac tggagttggc
     5221 atacagacgt tgtgttcctg gcgcccatgc aggctgccag ttttgataga agcgcagcaa
     5281 gtcgcttgag gatttcacct catccggcac ctgcaatggc aggccgccag cagtgtaggt
     5341 tgcgagatgt aatagtgtga tcccattcca ctgtttagcg gtaagttcag gccagtattt
     5401 tgttgtggga tcgcttaact tgatttcccc tcgagcaata gcgtcgccac caagcacgcc
     5461 cgtaaatgtt ttgctgaccg aacctaactc aaacaacgtt tgctgtgtga cgggctgctt
     5521 tttggcgatg tccgcatagc cccaggtaaa gtaataaggt ttaccctgat aaattaccgc
     5581 caccgccata ccggggatct tttgttgctc tataagcggg gtaattgtgc gatgcacaat
     5641 atcgttgatt tgttgagggg cagcaaatgt ggagcaagag gcggtaatta ataaggcgca
     5701 gagcgtcgtt ttgaacataa ggtctggttt ccatacaaaa cggcccgcca taggcgggcc
     5761 ggatttacat tggcgatacg ttagattgta acgataccaa tcagcgtgac aactgtcagg
     5821 atagcagcca gaccgtagaa aacccatttg cccgcaggca cgtgaatttt cagatcgtgc
     5881 atcgcgtggt gcatacggtg taaaccacac cacagcggca gaacgatcat caggaacagg
     5941 aatacgcgac caatgaagct ctgcgcgaac gccagaacgc gctcgtagct cagcgcatca
     6001 cccggaaaca gccccagtgg cagcagaata cccaccagca ggatcatcac cggcgcaata
     6061 attgcgctcc acataccacc ggccccgaag aggccccaga ataccggttc gtcagaacgc
     6121 tttggatttg gattaatcat ctcaggctcc ttaccagtac agggcaacaa acaggattac
     6181 gatggtggca accacagtta ccgcccagag acttttgata attggctctg gtcccatttt
     6241 ttcgtctttt acaatgatat tggccgcttt cggtgccagt tcaaaccagg ttttggtgtg
     6301 cagcagagct gccgccagag tgatcaggtt aatgatcacg ataaccgggt tttgtaaaaa
     6361 gtcgacgaat cccgcccagg cttccgggcc atttttcagg gcaaacagcc cgaaaatcag
     6421 ttcaatgctg aaccacacag ccggaaccgc cgtgccttcg cgcagcatgt aaaagcgata
     6481 aaacggcaat tttttccacc aggtggacgt cattggccgt acatacggtt tacgtttagt
     6541 cgtcatgttg cactccttag cgtggtttca gggtcgcgat aagaaagtct ttcgaacttt
     6601 ctactttgcc ctgctgaatg gccgcagccg gatcgacgtg tttcgggcag acttcggagc
     6661 aatagcccac gaaagtacag ctccatacgc cgttctggct gttcaactgc gccatacgct
     6721 ccttcttacc gtggtcgcgg ctatcttcgt tataacgatg cgccagcgta atggcagccg
     6781 gaccgatgaa ctctgggttc aggccaaact gcgggcacgc ggcgtagcac agaccacagt
     6841 tgatgcaacc ggagaactgg tgatactttg ccatctgcgc cggggtctgg atgttagtac
     6901 cctgatccgc ggtgcgggag ttgccgatga tgtacggttt gatcgcttcc agactttcga
     6961 tgaagtgggt catatcgacc accagatcgc gttcaatcgg gaagttagct aacgcttcaa
     7021 ccttcatacc gtcggtgtaa tcacgcagga aggttttaca tgccagtttt ggcacgttgt
     7081 taaccatcat gccgcaggaa ccacaaatcg ccatacggca ggaccagcgg taactcaggt
     7141 ccggtgccag gttgtctttg atgtagccca gcgcatccag taatgaggta gttgcgtcat
     7201 aaggcacttc atagaatgcg ctatgcggtg cggtatcgac ttccgggtta tagcgcacca
     7261 cctcaatttt caggtttttc atctcagcca ttcgccttct ccttcttatt ggctgcttcc
     7321 gccttatcgg ctgcatccgc ttcgccaccg taaacgcgtt tagctggcgg cagcgtagta
     7381 atcttcacgt cgctgtactc caggcgagtc gtgccatcag catcgcggaa ggcgagggtg
     7441 tgtttgagga agttgacgtc gtcacgctcg gtgcaacctt cgtccagacg ctggtgtgcg
     7501 ccgcgggact ctttacgtgc cattgcggag tgcgccatac attcagcaac gttcagaccg
     7561 tggcccagtt caatggtgta gagcaggtcg gtgttgaaca cgctggaggt gtcggtgatg
     7621 cgcacgcgct tgaagcgttc ctgcagctct gccagcttgt cgatggtttt ctgcatcagt
     7681 tccggcgtac ggtagatacc gcaaccttct tccattgcca ggcccatttc gtcgcggatc
     7741 ttcgcccagt tttcgccgcc atcctggtta accagatctt tcagacgttg ttcaacgcca
     7801 gctgcctgcg cttcaattgc cgcttcgttg ccattaccgg cagttgctgc aagctctgtc
     7861 gcttgttcac cggccagacg gccgaagacc accagttccg ccagggagtt agaacccaga
     7921 cggtttgcac cgtgcagacc aacagaggaa cattcaccca cggcgaacag acctttaatg
     7981 cgggtttcac agttctgatc ggtttcgata ccgcccatgg tgtagtgtgc ggtcggacgt
     8041 accggaatcg gttctttaac cggatcgacg ccaacgtacg ctttcgccag ttcgcagatg
     8101 aacggcagac gttcatgcag ttttttctcg ccgaggtgac gcaggtcgag ataaaccaca
     8161 tcgccacgcg gcgtggagat ggtgttgcct ttacgccatt cgtgccagaa ggcctgagaa
     8221 actttgtcgc gtggacccag ttccatatat ttgtttttcg gctcgcccag cggagtttcc
     8281 gggcccatgc cgtaatcttg cagataacgg tagccatttt tgttgaccag aataccgcct
     8341 tcaccgcggc agccttcggt catcaggata ccggaacctg gcagaccggt tgggtgatac
     8401 tgaacgaatt ccatgtcacg cagcggaacg ccgtggctta gcgccatacc cataccgtca
     8461 ccggtaacga tgccgccgtt ggtgttgtaa cgataaacgc gacccgcacc gccagtagcc
     8521 ataacgaccg cgttagcacg gatctgcacc agcgtgcctt ccatcatgtt cattgctacc
     8581 acgccgcgaa catgaccatc atcaaccaga atatccagca cgaaatgttc gtcaaaacgc
     8641 tggatctgcg ggaattgcag agaggtctgg aacagcgtgt gcagcatatg gaagccggtc
     8701 ttatcggcgg cgaaccaggt gcgctcgatt ttcatgccgc cgaagcgacg tacgttgacg
     8761 ctaccatccg ggcgacggct ccatgggcac ccccacagtt ccagttgggt catttcggtt
     8821 gggcagtggt ggacgaaata atccacgaca tcctgctcac acaaccagtc gccacccgct
     8881 actgtatcgt gaaagtgata ttcgaagctg tcatgatcct gcgcgacagc ggcggagccc
     8941 ccttctgcag caacggtatg gctacgcatc gggtatactt ttgagattag tgcgattttt
     9001 gcatttggat ttgcctgcgc ggcagcaatt gcagcacgta atcccgcgcc accggcgcct
     9061 acaatggcaa gatcggcttg aaaggtttgc acgacattcc tccagattgt ttttatccca
     9121 cagccccgta cttcagggta agtgcctgaa agttacggtc tgcgaacgct attccactgc
     9181 tcctttatag gtacaacagt atagtctgat ggataagtct gaaatttgac gagatcgatt
     9241 tttttagtgc gcaagagggc taaattatca ctgaagatga ttaatttaat tactaaacca
     9301 tcagatcgtg ctttttttag tcactgccca ccgctgtttg attcctgcgt aaaatttgtc
     9361 tcgccgtcgc gttgcgagta gacttcgtgc ccttgtcaaa aactggagat ttaactatga
     9421 gcgaaacggc atcctggcag ccgagcgcat ccattcctaa cttattaaaa cgcgcggcga
     9481 ttatggcgga gatccgtcgt ttctttgccg atcgtggagt gctggaggtg gagacgcctt
     9541 gtatgagcca ggcgacggta accgatattc atttggtccc gtttgagaca cgtttcgttg
     9601 gccccgggca ttcgcagggg atgaatctct ggttaatgac cagcccggaa taccatatga
     9661 aacgcctgct ggttgccggt tgtgggccgg tattccagct gtgccgcagc ttccgtaatg
     9721 aagagatggg gcgttatcac aaccctgagt tcactatgct ggagtggtat cgaccgcact
     9781 atgatatgta ccggttgatg aacgaggtgg acgatctctt acaacaggtg ctggactgcc
     9841 cggcagcaga aagcctttct tatcaacaag ctttcttgcg ttatctggaa attgacccgc
     9901 tctctgccga caaaacgcaa ctgcgggaag tcgcagcgaa actggatttg agcaatgttg
     9961 ctgataccga agaagaccgc gacacgctgc tacaattgct gtttaccttt ggcgtagagc
    10021 caaatattgg caaagaaaaa ccgacctttg tgtaccactt tccagccagc caggcatcac
    10081 tggcgcaaat cagtaccgaa gatcatcggg tcgctgaacg ctttgaggtt tattataaag
    10141 gtattgagct ggcgaatggt ttccatgaat tgacggatgc ccgtgagcag caacaacgct
    10201 ttgaacaaga taaccgtaag cgcgcggcgc gcggtttgcc gcagcacccc attgaccaga
    10261 atctgattga agccttgaaa gtcggtatgc ctgactgttc cggcgtggca ttaggtgttg
    10321 atcgtctggt gatgttggcg ctgggcgcgg agacactggc tgaagtcatc gcctttagcg
    10381 ttgaccgggc ataattctga aaaattactg aattaacagc gaagaatggc gtgataagcg
    10441 ccattctgta gcatatgttt tcatttttcc gttaaaggcc acaatttaag actggatatt
    10501 tgatatcatc caggtatcaa ttctgtattg gtttttacgc tgcctgctca atgttgcgca
    10561 gtgcagttca gtgggcacgt gttatacacg cgctgaaatg aaggatggtt tcatgcctca
    10621 cacgataaaa aagatgagtc tgataggact catattgatg atctttactt ccgtatttgg
    10681 atttgccaat agcccatcgg cttattactt aatgggttat agtgcgattc ccttttatat
    10741 attttctgca ttgttattct ttattccatt cgccttaatg atggctgaaa tgggagctgc
    10801 ttatcgcaaa gaagaaggcg gtatctattc ctggatgaat aatagtgtcg gaccacgttt
    10861 tgccttcatt ggtacgttta tgtggttttc ctcttatatc atctggatgg tgagtacctc
    10921 cgcgaaagtt tgggtaccgt tctcaacatt cctctatggt agcgacatga cccagcactg
    10981 gcgtattgct ggactggagc ctacgcaggt ggttggtctg ctggcagtgg catggatgat
    11041 tctggtcacc gtcgttgctt caaaggggat taataaaatt gcccgcatta ctgcggtggg
    11101 cggtattgca gtaatgtgtc tgaatttagt attgctgtta gtaagcatta ctattttgtt
    11161 attaaatggt gggcatttcg cgcaggatat taatttcctt gcatcaccga acccaggtta
    11221 tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct atttttgcct atggcggaat
    11281 tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg
    11341 tattgttttt gccgctattg ttatttcaat cggttattcg ctggcaatat ttttatgggg
    11401 cgtcagcaca aactggcagc aggtattaag taatggttcc gttaaccttg gcaatattac
    11461 ctatgtgctg atgaagagcc tcggggtgac gctgggtaac gcactgcatt tgtcacctga
    11521 agcgtcattg tcgctgggcg tatggtttgc gcgtattacc ggactttcga tgttcctcgc
    11581 ttataccggt gcgttcttta cgctttgcta ttcaccgctg aaagccatca tccaggggac
    11641 gccgaaagcg ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga tgccgtctat
    11701 cgccatgtgg atgcagtgcg ggttggttac tgtcttcatc ctgctggttt cgtttggtgg
    11761 cggtaccgca tcggcgttct ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct
    11821 tccttacctg ttcctcgcgc tggctttccc attttttaaa gcacgtcagg atctcgacag
    11881 accatttgtg attttcaaaa cgcatatgtc ggcaatgatt gcaacagtgg ttgtcgtact
    11941 ggtggtgaca tttgcgaacg tcttcaccat cattcaacct gtggttgaag ccggagactg
    12001 ggacagcaca ttgtggatga ttggcggccc tgtcttcttc tcgctgttag cgatggcgat
    12061 ttaccagaac tattgcagca gagtggcaaa aaatccgcag tgggcggtgg aataacccga
    12121 tgccctatcg tctacctgac gatagggcgt aaagacagga aatgttatgg atgacaccag
    12181 ccgggacccg gcaataaccg aagatgaaat cagggcgctg caattttctg ccggggatgt
    12241 agcggaaata gagcagacga ttctctcttt tgtcgatgcc tgccacaccc gtaaagtggc
    12301 gatggtggtg ggtagcacca ttaacaccct caaagatcgc gatggtaaac ggtggggaaa
    12361 cctgccggat atttattgcg cttatcttat ccgatgtctg gtttttcgcg gggaattagt
    12421 gggatacggc gatttgtttc gtatgcgcta tagcgaaatt aagcgaccgg ttaccttatg
    12481 agtgcgcgca tgtttgtctt atgctgcatc tggtttattg tggcgttcct ctggataacc
    12541 atcacttccg cgctggacaa agagtggatg attgatggac gcgggattaa caacgtctgc
    12601 gatgtgttga tgtatctcga agaagacgat acccgtgatg ttggtgtgat aatgacgtta
    12661 ccgctctttt tcccttttct ctggttcgct ttgtggcgaa aaaaacgcgg ctggtttatg
    12721 tacgccaccg cgctggctat tttcggctac tggctgtggc agttttttct gcgctatcag
    12781 ttttgtttgt gagccggatt ggttcatccg gcacacaaac ttacaaactt cccgcctgac
    12841 gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg ctttccagac
    12901 gcatctggaa gggcgggaat ggcatatcga taccgtgggc gtggaagcct gccagaatca
    12961 gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggcg taaatacgca
    13021 gctcgaaaat ctgaatcccc tgttgcagat ccaccaggaa gacttccggt gccgggttgt
    13081 cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact tcttcgctat
    13141 tggcatcggc aggggccggt atcgtcaaca ccacgcgcgt gaccgagtca gagagcgacc
    13201 agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct ttacggtccc
    13261 agtcgctgat ggtggtggcg cgggtgttaa ttttcgtcac gctaccggta agatcgcgga
    13321 tcgttaccgt atcgccaatg cgaatcggtt tttcgaacag gataatcagg ccagagataa
    13381 agttggcgaa aatctcctgc aaaccaaaac cgagaccaac accgagcgcg gcaaccagcc
    13441 actgcaattt cgaccactca ataccaatca tcgagaagcc gaccagcccg ccaatcagca
    13501 tcagcagata tttggtgatg gtggtgatgg cgtagcccgt acccggcgtt aaatccaggt
    13561 gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg caccagctgc gtggtgatga
    13621 taaacaccag aatggcaatc agcaccgcac cgagggtaat tggctccaga ctttctacgc
    13681 cctgtaccgt ggaggtgaca tcccacagcg aaatattttc gaggaagccg aaagcggaat
    13741 ggatttctga ccacagcaca atgacagaaa gcagggcgat caacatcaaa attgagcgca
    13801 ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca tcgacttcaa
    13861 ttgccccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgcg cgttgcgcta
    13921 acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcgacgc tggatcagca
    13981 tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca accgaggttt
    14041 caagcctcgc taacagtgcc tgcgccgttg ccagataacc caccgctgac gccagaatgg
    14101 caaccagtgg tgcgccaatc atcatgttcc acagcatatg gttggtaatg ttgtcgccgc
    14161 tgccctcttt gttgaggtac agcgggatcc cggccttttt caggctgagg gtgaccaccg
    14221 ccagcgcacc gcaaatgagg ataaagcaaa gccgtcccag cgaaccggag aattcacggt
    14281 cgtcgaggtt atcgaacatc atcagcgcca taatcagcgg cacaataagc ccgatgctca
    14341 tcaggtagta gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa
    14401 acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg
    14461 tggccgttac gccatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc
    14521 cgtaacccag cgtcatccac agcaccggca gcggtgacgc gacgagaatc gaccagaaaa
    14581 gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac
    14641 gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg
    14701 cgccaaacag cggcaaaatc gtctctttgc tggtcagcat catcacactg gctttgccca
    14761 actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagtggcc
    14821 aggcgatggt catcgggcgc acgtcagagg tccagaacag atagcgatgc gtcgcttcat
    14881 tcacctcttt cagcgcatcc tcaagttgcc cgttggagac tttcagcttg gtcagttcca
    14941 gcagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgtagct
    15001 gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatgaattt
    15061 gccgtagcag cggctgttta ttaagcagat cctcataacg taaccgttgc acacgcaact
    15121 gcgccatttc ggtatcaagc tgttgtggtt tcggcatttc cggcagccgt gccacctgcg
    15181 cccgcaacgc ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg
    15241 tattcagcgc ctgccgaacc tgtaacgtct ggctggtggc ctggcgctgt tgcgaggcaa
    15301 cgagatccat ccgctgcgcc tgttgattca gtgccgccga tagttcgcgg ttaattttga
    15361 attgcgcgac gatgtctttc ggcaaatcgg cgctgttttc tgccagcaat tcggtacttt
    15421 ccagcgcccg ctccgcctca agctgacgtt ggctgttcaa ctgattacgt aaggcctgca
    15481 aatacgcatc cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt
    15541 cctgacggtt attggcagac agctgcgcca gttccagttc atcaacgagc gctttcaggc
    15601 gtgcggagtc agactgcaac gcgaaatttt gtgcctgatt gagcggggta ttgccagtaa
    15661 gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg
    15721 gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac
    15781 ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag
    15841 acattcccgg cgacacgctg cgcggctcgt cacgcatgtt gtttaattgt gcgcgcagag
    15901 tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg
    15961 aaccttttcg ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct
    16021 gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg
    16081 ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc
    16141 gcacgtcagc gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc
    16201 tggcgtcgtg ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg
    16261 taacaaaggt ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt
    16321 ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg gtagagccga
    16381 gtttaaagcg gcccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg
    16441 caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga
    16501 ccgtctcaat gctgcctaca atcgtcgccc cgaccagaat ctgcgccatc gggccaaatt
    16561 cggtatcgaa caggcaaatc acgcgttcgt tacgggcaaa cagattcggc acgttctgag
    16621 cggtgagatg gttaacggag aagagatcgc ccggcacgta gatcatctca cgcagaatac
    16681 cgttgcacgg catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg
    16741 taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt
    16801 agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa
    16861 cgccatcggc aggcatgacc aggacattcg gatcggtatc gattgggcgt acttcgtcac
    16921 gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg
    16981 cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc
    17041 atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa
    17101 tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc
    17161 gttcctgatc cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt
    17221 agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc
    17281 tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg
    17341 cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac
    17401 ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga
    17461 ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca
    17521 gccgagcggc ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca
    17581 ggatctcttt ttgcagcccc agcaacgcat tcagcaggct ggatttgccg acgccagact
    17641 gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttt agcccatcct
    17701 gagtatggct ggaaaccatc aatacgcgat aaccgatatt gcggtagata tccatctgct
    17761 cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acaataatcg
    17821 gctcaatctg caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct
    17881 ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggcaata ggttttacgc
    17941 cgtcgtagaa atccggacgc gtcaacaccg aggtacgctc atgcaccgct tccacgatcc
    18001 ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg
    18061 ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt
    18121 ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca
    18181 gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt
    18241 tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtaca gactcctggt
    18301 cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg
    18361 aatgggtgga aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga
    18421 tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg
    18481 ccaacctgaa tattctggca gaagggccga ccattgcagt acaccagtct gatgaacagc
    18541 tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg
    18601 tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa tttttaaaac
    18661 agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggtcag gaccgtcgtt
    18721 tcctgtttaa atacatgccg gagctggaag cctacttcca ctaccgttat ctcgatgtca
    18781 gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc
    18841 aggggacgca tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc
    18901 gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg
    18961 cggcggcgtg attttgacgc taaattgaac gtcttgctga taatttcagc acttgagata
    19021 aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc
    19081 ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa
    19141 tagctcagtt ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt
    19201 cccgctccaa aatttgaaaa gtgctgcaaa gcacagacca cccaatcgca gtatttcgaa
    19261 ttgcgacaag gcggcaactg aacgaatcct tgggaactta caaaagtatg tgactaaggt
    19321 gagtgaaggc agccaaggca gtagcggttc gaaagacgaa gattaagcgg gaatagctca
    19381 gttggtagag cacgaccttg ccaaggtcgg ggtcgcgagt tcgagtct
//
LOCUS       DAZFJF010000087        19416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFJF010000087 DAZFJF010000000
VERSION     DAZFJF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.087"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..357
                     /locus_tag="ACHKAU_003376"
     CDS             76..357
                     /locus_tag="ACHKAU_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5532372.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            323..1438
                     /locus_tag="ACHKAU_003377"
     CDS             323..1438
                     /locus_tag="ACHKAU_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5532373.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMDLALVTGQRREDITN
                     MRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYYCSDFNVNGLLNVFWL"
     gene            complement(1791..2132)
                     /gene="yebY"
                     /locus_tag="ACHKAU_003378"
     CDS             complement(1791..2132)
                     /gene="yebY"
                     /locus_tag="ACHKAU_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO5532374.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2145..3017)
                     /gene="copD"
                     /locus_tag="ACHKAU_003379"
     CDS             complement(2145..3017)
                     /gene="copD"
                     /locus_tag="ACHKAU_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO5532375.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(3021..3395)
                     /gene="yobA"
                     /locus_tag="ACHKAU_003380"
     CDS             complement(3021..3395)
                     /gene="yobA"
                     /locus_tag="ACHKAU_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO5532376.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3534..3764
                     /gene="holE"
                     /locus_tag="ACHKAU_003381"
     CDS             3534..3764
                     /gene="holE"
                     /locus_tag="ACHKAU_003381"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO5532377.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3866..4522
                     /gene="yobB"
                     /locus_tag="ACHKAU_003382"
     CDS             3866..4522
                     /gene="yobB"
                     /locus_tag="ACHKAU_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO5532378.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4546..5208
                     /gene="exoX"
                     /locus_tag="ACHKAU_003383"
     CDS             4546..5208
                     /gene="exoX"
                     /locus_tag="ACHKAU_003383"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO5532379.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5205..7265)
                     /gene="ptrB"
                     /locus_tag="ACHKAU_003384"
     CDS             complement(5205..7265)
                     /gene="ptrB"
                     /locus_tag="ACHKAU_003384"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO5532380.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7474..8133)
                     /gene="yebE"
                     /locus_tag="ACHKAU_003385"
     CDS             complement(7474..8133)
                     /gene="yebE"
                     /locus_tag="ACHKAU_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5532381.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8460..8816)
                     /gene="yebF"
                     /locus_tag="ACHKAU_003386"
     CDS             complement(8460..8816)
                     /gene="yebF"
                     /locus_tag="ACHKAU_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO5532382.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8883..9173)
                     /gene="yebG"
                     /locus_tag="ACHKAU_003387"
     CDS             complement(8883..9173)
                     /gene="yebG"
                     /locus_tag="ACHKAU_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO5532383.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9307..10485
                     /gene="purT"
                     /locus_tag="ACHKAU_003388"
     CDS             9307..10485
                     /gene="purT"
                     /locus_tag="ACHKAU_003388"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO5532384.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(10541..11182)
                     /gene="kdgA"
                     /locus_tag="ACHKAU_003389"
                     /gene_synonym="eda"
     CDS             complement(10541..11182)
                     /gene="kdgA"
                     /locus_tag="ACHKAU_003389"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5532385.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11219..13030)
                     /gene="edd"
                     /locus_tag="ACHKAU_003390"
     CDS             complement(11219..13030)
                     /gene="edd"
                     /locus_tag="ACHKAU_003390"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO5532386.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13265..14740)
                     /gene="zwf"
                     /locus_tag="ACHKAU_003391"
     CDS             complement(13265..14740)
                     /gene="zwf"
                     /locus_tag="ACHKAU_003391"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO5532387.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15078..15947
                     /gene="hexR"
                     /locus_tag="ACHKAU_003392"
                     /gene_synonym="yebK"
     CDS             15078..15947
                     /gene="hexR"
                     /locus_tag="ACHKAU_003392"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO5532388.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16075..17517
                     /gene="pyk"
                     /locus_tag="ACHKAU_003393"
     CDS             16075..17517
                     /gene="pyk"
                     /locus_tag="ACHKAU_003393"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO5532389.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17577..18419)
                     /locus_tag="ACHKAU_003394"
     CDS             complement(17577..18419)
                     /locus_tag="ACHKAU_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFO5532390.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYADLEGDVLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEP
                     DSAINITKRFAKNLKIYNLPGIQFYGDRARIVRTIGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGYSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
     gene            complement(18442..19281)
                     /locus_tag="ACHKAU_003395"
     CDS             complement(18442..19281)
                     /locus_tag="ACHKAU_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO5532391.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPLNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
BASE COUNT         5073 a         4831 c         4615 g         4897 t
ORIGIN      
        1 ctgattatta aaaatcaacc acacccgcca gtattctgta tatttactgg cggtcatatc
       61 gtaagaggta tggcaatgaa tcttgtgaca ctcaaaacgt ggggaaaact cagatatccg
      121 gataacccac catcaatatc aacgctgaga cgatgggcaa ggaatggaaa catttatcct
      181 gcacctgaac tacacgggag gagttacagg gtggttccgg aagctttcta tatcaaccca
      241 aataaggttg ataccgatat aacacaccat cagcctaatg ggcgacaagg gagagacagt
      301 ccgttactgg agaagttaaa acatgcagcg gaaaaaatac gatcccaatt tacctaaaaa
      361 cttaacatat cgaaggaggg acaaagcata ttactggcgc aaccctctga cgaaagaaga
      421 atttacacta ggtaaaattt caagaagaga tgcagtagcg caggcaattg aagcaaatca
      481 ttatatatac aaaaactact ctcctgctgc cttaattgaa aaactaaaag ggttcgactc
      541 atttactatg gcagactgga ttgaacgtta caaaacgatt cttataagga gaaaagtgtc
      601 cagaaatact tataaaattc gggtaaatca actggagaca ataaaagaaa aattaggagg
      661 ggttttactg acagaaataa ccactcgcca tattgccgag tttcttgatt tgtggattga
      721 aggagggaaa aacacgatgg caggatcaat gcgttctgtg ttatctgata tgttccgcga
      781 ggccattgtt gaaggacgta tatctcaaaa tccagtaacg ccaacaagag caccgaaaat
      841 agtagttaca agagaacggc tgaaactaaa gacatacaac tgcatcaggg aggcagcaga
      901 tcaacttccg acatggttcc cattagctat ggacttagcc cttgtaacag gacaacgtcg
      961 cgaagacata acaaatatgc ggttcagtga gatttatgat gatcgtctcc acatcaggca
     1021 aattaagaca ggaatgatga ttgctatccc cctgtcactc agccttcctg tcgctggttt
     1081 acgacttggt acagtagttg aacagtgccg catggtaagt aggggggatt atctaatcag
     1141 tgccgggatt agaaaaaaca gccctgacgg cagcattcac ccggacggcc tgacaaagaa
     1201 atttgtcgca gccagaaaat taacaggtat ccagttcagt gaaaacccac caacttttca
     1261 cgagatcaga agcctggctg gacgattgta caaagaaaca tgtggggaag aatttgctca
     1321 acgtctactt ggccacacat cggagaagac aacaaaaatg tatcttgatg agagagaaaa
     1381 aacgtactac tgctctgatt ttaacgtaaa tggattgtta aatgtgtttt ggttgtgata
     1441 taaccaaaaa agaccggaat acagaaattc gagtaaattt cgagtaattt cggggagacg
     1501 tttgcaacta attgatttta aatacaatta aaaaaagacc gaatacgatt cctgtattcg
     1561 gtccagggaa atggctcttg ggagagagcc gtgcgctaaa agttggcatt aatgcaggct
     1621 tagttgcctt gccctttaag aatagatgac gacgccaggt tttccagttt gcgtgcaaaa
     1681 tggtcaataa aaagcgtggt ggtcatcagc tgaaatgtta aaaaccgccc gttctggtga
     1741 aagaactgag gcggtttttt tattggaaat caaaaggcta ttttaggtaa ttaacagagt
     1801 ttttcagctc gttctataaa cggtgccaga ctcatttttt cgccgggatt gttaggatca
     1861 tcaatctgaa tcaccgaaat gggttgggct ttagtcttcc cactggcaac ttccttttgt
     1921 gcgatatcgt ttaaaggata ctgcacgagg gtacttgggt taatgacata caaagcatta
     1981 cccggtctgc aagtcagcat cacctcttcg cgattaaacg cccatttgtc tttacccact
     2041 tcaaaacgac tgacggtaat cacctgcggt gcagccagcg ccgctgcaga actggtgagt
     2101 aacagaaacg tcagaatact ttttttcatc attttcgtaa tccatcaaaa aggttcgaga
     2161 gtcgcaagca ggctgatgat caccagcact accgcaccta tcgcccattc gagtttagtc
     2221 atccactcaa accagggtgc cgcacgatct tcatcttgcc tcatgcgcgg tacaagaaca
     2281 taacgattag ccagcgcaat gaccaccata atcatcacca ggatggcttt gagcaataag
     2341 agttggcccc agtaagtcgt aagcgtgggg ggaaatccgg taatcaacaa tgcattaagc
     2401 acgccgcttg ccagtacgcc gatcaccgca aaatgcccgc accaggaaaa acgcatcagc
     2461 gcctgaatgg cctggtgtcg ccaacggcct ttgatgagtt gcatacacca gagcaccggc
     2521 agtaatcccc caaaccacgc cgccgcacaa atcaggtgaa tcgcgtgatt agtctgatgg
     2581 attttcgccg ttaccccttc attcagtgtc gcatgcccga ttcccgccag cagaataaat
     2641 tgcgcggtag tgagcataaa cagcaaacgt ggcatattcc gcggttgcat aagggcgacg
     2701 atcaacgtta cgagggcgag aacaatttgc cataaccaga cgccaccaaa ctgcgtttgc
     2761 aaaacagcct gccaaatgtt tggcgaaaat acatctgccc atcccgttcc catcaacccg
     2821 ccctgaacgg cgagcattgc agtggcactg atcaaactcc agacggcagc gtgctgttgt
     2881 aaacgcagaa aacgcttcgc cagcaagcga cgaatcgtta agggggccag ccaggcaccg
     2941 tacattgcga agccaaagac cagcatcagc gaggtgaaat ggataaatcg cagcgcgatc
     3001 caggtaaacg ccagcatgat ttatttcacg ctaaaggtgt agtgtccttt cgttttatgc
     3061 ccgtccaccg aaacaacatg ccagtcaacg atgtaggttc cgggtttcag cgaatcggcc
     3121 agtggaacaa tcagttgctt ttggtcctgc tcatttcgct tcgccggtaa tgttttaata
     3181 ttttcgtttt ttggccccgt gatttttgca ccactgaatc ctgtttcaac accttccgag
     3241 aagtttaaag tgattgcctg cggtgcagct gtcacttgcg cgtttgccgc aggatactga
     3301 tgcgttaaat gcgcatgtgc ccagacggaa ggtgtcacca acgaagtggt caggattgcc
     3361 agcgcgtagc gaagggagcg tgcagttgaa gtcatattat ctattccttt ttgtaataac
     3421 ttttttacag agcataacct tgtctaatgt ctgagtcgag gatcatcaat tccggcttgc
     3481 catcctggct cactcttagt aacttttgcc cgcgaatgat gaggagatta agaatgctga
     3541 agaatctggc taaactggat caaacagaaa tggataaagt gaatgtcgat ttggcggcgg
     3601 ccggggtggc atttaaagaa cgctacaata tgccggtgat cgctgaagcg gttgaacgtg
     3661 aacagcctga acatttgcgc agctggtttc gcgagcggct tattgcccac cgtttggctt
     3721 cggtcaatct gtcacgttta ccttacgagc ccaaacttaa ataaaactta tacagagtta
     3781 cactttctta cataacgcct gctaaattat gagtattttc taaaccgcac ccataatttg
     3841 cagtcatttt gaaaaggaag tcattatgtc gttctggaaa gttgcagcag cgcaatatga
     3901 gcctcgtaaa acctcactca ccgaacaggt agctcatcat ctggagtttg tgagggccgc
     3961 cgccaggcaa caatgtcagc tgttagtttt tccatcgctt tctttactgg ggtgtgatta
     4021 ttcgcgacgt gcccttcctg ccccacccga tctctcactg ttagacccgc tttgttatgc
     4081 cgcaacgacc tggcgaatga ccatcattgc cggccttccc gttgaatata acgatcgctt
     4141 tattcgtgga attgcggtgt tcgcaccgtg gcgaaaaacg cctgggatct accatcagag
     4201 tcatggtgca tgtctgggtc gtcgttccag aaccatcacg gtggtcgatg aacaaccgca
     4261 aggtatggat atggacccga cctgttcact gtttactaca gggcaatgtc tgggagaacc
     4321 cgacctgctg gcgtcggccc gtcgcttaca atttttttca catcagtact cgattgccgt
     4381 gctgatggca aatgcccgtg gtaacagtgc actatgggat gaatatggtc gtctcatcgt
     4441 tcgcgccgat cgtggttcat tattgttagt cggtcagcgt tcatcacagg gttggcaagg
     4501 cgatatcatt ccattacgct aggctttttc ggcctggagc atgccatgtt gcgcattatc
     4561 gatacagaaa cctgcggttt gcagggaggg atcgttgaga ttgcctctgt tgatgtcatt
     4621 gacggaaaaa tcgtcaaccc catgagccac ctggtgcgcc ccgatcgtcc tattagtcca
     4681 caagcgatgg cgattcatcg catcaccgaa gccatggtcg ccgataaacc gtggattgaa
     4741 gatgtgatcc cacactatta cggtagtgaa tggtatgtcg cgcataacgc cagctttgac
     4801 cgccgcgtac tgcctgagat gcccggtgag tggatttgca ctatgaaact ggcccgtcgt
     4861 ttgtggcctg ggatcaagta cagcaatatg gcgttatata aaacacgcaa gctcaatgta
     4921 cagacgccgc cgggcctgca tcatcaccgc gcgttgtatg actgttatat caccgccgcg
     4981 ttgcttatcg atattatgaa cacctccggc tggacggcag aacagatggc cgatatcacc
     5041 ggacgtccgt cgttgatgac gaccttcacc tttggcaaat accgtggcaa agcggtttcc
     5101 gacgttgccg aacgcgatcc gggctatctg cgctggttat ttaataacct ggacagcatg
     5161 agcccggagc tgcgtttaac actgaaacat tatctggaaa atacttagtc cgcaggcgta
     5221 gcgggtaatg ttccctgcgc cagcgcgacc agaaaagcat attccatcgc tacgccttcg
     5281 tacgatttaa agcgaccaga tttaccgcca tggcctgagt ccatgtcggt acagagcaat
     5341 aaaagatggt catcggtttt cagctcgcgc aatttagcga cccattttgc cggttcccaa
     5401 tattgcacct gagaatcgtg caaaccggtc gttaccagta aatgcggata agcctgtgcg
     5461 gtgacgttgt catacgggct gtagcttttc atgtactcgt aatattgcgg atcctgcgga
     5521 ttaccccact cttcaaactc accagtggta agaggaattg attcatcaag catcgttgtt
     5581 acaacatcaa caaacggtac ctgggcgata acgccgtgga ataattccgg gcgttgatta
     5641 attgcaacgc ccatcaacat gccccccgca ctcccgccca tcgcataaca aagcgaagga
     5701 gagccatagc ccagttttaa caatgcatcg caggcatcaa gataatcatt aaacgtattt
     5761 ttcttcttca gaaattttcc gtcttcgtac cattgttgcc ccagctcacc accgccgcga
     5821 acatggacaa tggcgtagac aaagccacga tctaacaaac tcaagcggct aaaactgaaa
     5881 tcggcatcaa tacttgcgcc gtaagaacca tagccataca ccagcaacgg gttgtgtcct
     5941 ttgcgaaaat gtttgcgatg gtagaccaac gaaaccggaa cttcgacgcc atcacgggcg
     6001 actatccaca ggtgttcact gcggtaattc gccgcatcaa aaccgggcac ttctgtttgt
     6061 tttaatacac gacgctcacc ggtatccata tccagttcaa acaaagtgtc tggtgtggtc
     6121 atggaagaat aaccataacg caatcgcgcg gtttcaggtt ctggattgta ggcaatccag
     6181 gtcacatagg ctggatcatc aaaggcaata ccaatgactt cccgggtctt gcggttaatt
     6241 tggcgcaaac tggttaaccc gcgctgacgc tcttcaacca ccagccagtc ggtaaacagc
     6301 gtaaaccctt ccagcatgat gttttcgcgt ggcggaatta actcttccca ctgttgctca
     6361 tcacgcatac gggtacggta taagccaaag tttttgccgt ggcggttgga acgtagataa
     6421 aaacgatgct ggtagtgatc aaggctgtat tcgtgatctt tgcggcgcgg cagaaaaaca
     6481 aacggctcgg catcggccat ttccgcgtcc agcaggcgaa cttcactggt ggtggcgctg
     6541 gccaaatgaa tgactacata gtgcttcgag gtcgttttat gcaggctgac gtaataggta
     6601 tcgtcttttt cttcgtagat cagtttatct tgcgatgctg gcgtaccgat ggcgtgacgc
     6661 cagacctgat aaggcagcag cgtcaccgga tgcttgcgaa catagtagaa agtccaggag
     6721 tcatttgccc agacaaagct gggttcaacg ttatccagca gttccgggta ccagttacca
     6781 gtttccagat tacgaaaacg aatgccgtac tggcgtcggg aaagaaaatc ttctgccagc
     6841 gccataatgg tgttatcggg cgtaatcgcc attccgccca tcgaataaaa ctcactatga
     6901 gctgcgcgct tattggcatc gagcaatgtt tcccactcat cccactcttc actgaatgcc
     6961 gattgacgct ggtagatagc atattcacag cctggttcat aaatatgccg atagcggtag
     7021 ccatttttga tgtagggcgc agaaacttct cgttgcggaa tgcggtcgat gatttccttt
     7081 aagatgcgat cctgcaaggc ttgttgtgag gccatcaccc gatgaccgta actattttct
     7141 tgttgcaggt agtccaggac ttctggctga gaacgcgtat cgtcccgcag ccagtagtaa
     7201 ttatcgatgc gcgtatcgcc atgaagcgtc atggcgtggg gaatgcgggc ggcttttggt
     7261 agcatgttat tgttctttct ggttgaaaca tcttataagg gtggcaaaac tcaccgggga
     7321 tgcaagcgaa acaggggagt cattgcttag atgatgacag gtaatggcgc ggatatcgaa
     7381 tgttatgcag acagagaaaa tcagcctgtt caaatggctg tgcgattctg gatagcccga
     7441 aatagtcaac ttcaggctat ccagagagcg gaattattcc gccaaagtgc gtttttgctg
     7501 ttcgagatcg cgttcaatgc cgtcacgaac atcctggggg attttcagcg cgtcacccag
     7561 tgcattcagg taactgcgtt ccataaaatg gtcaatatca atagccgcgc aactcaggaa
     7621 atagatttcc agcgcctctt cttcattgcg gaccccggta gccaggcgtt gtggatccag
     7681 cggttgttcg attgcctgct caatgagtac acgcccctgc tcttccacgc cggcttcacg
     7741 caattgctgg tcgattgccg cacgttcttt ggcatcaata tgaccatcac ttttagcggc
     7801 aaagaccagc gcaaggatca aacgttctgt acgctcatcc agcggcgtac tttgcgcgcc
     7861 aaactgcggt tcgtcctgat gcgccgcgcg aattttatct ttgtatttat tccacagcac
     7921 cgtacccgct accgctccgc cgccaaccag taacgcgttg gtgccatatt tcgtaagaag
     7981 tttacgtgct gatttatttg cgaccagcag cccagccagt ccgcctaatg cgcctggcac
     8041 taacagtttg cccaatccct gatccgcaga cgaggaggta gaagaactgc tttgcccaag
     8101 cagggattgc agttgattta accagttagc catatttgct cctcaataac catttttagt
     8161 gcctgtagca tagcggatga ggatgtcaga aaatgtatat cgagacaaaa gatgcgcaaa
     8221 taagcggggc ggtaggccgt tattcaaaga aattcgcgcc aggtaaggta tttactgtat
     8281 atcaggtgat aagggtattt taggtgaaag tagtttgtcg ttttgagtgg cattgtttga
     8341 tgctgattgc ccgatgcgac gctgttgcgt cttctcaggc ctacgattgt ggtgcgttct
     8401 ggggcggaca aggcactcgc gccgcatccg ccaacggtca atcgaattat cagaagccat
     8461 taacgccgct gatattccgc cattcccgct ttgcagtcca cgctgacctg gtaatgaata
     8521 tcggcacttt taccacgcac ggttagcggt actgaccatt tatcatcttt accctgaatg
     8581 tcctgcaaac tgacccaagc cacgggatcg gcctgaccga caattttttg atcatctgcc
     8641 caacgcgcca cgcgattttg ttgataatca cgttttacgc tcgcggcaat tccggcagca
     8701 tccagacctt cacactttgg gaaagtgacc gacttgctgg tttcattatt ggcagcgaaa
     8761 actgatgcgc aggcagaaac caacaacagc cctaaaaacg cccctctttt tttcatgttt
     8821 ttctccatag cacaatgatt caggagaaag catggtacaa attgtcagga gcgcaagttg
     8881 cttcaggccg cgtgtgaggc atcttcctct tcggattcag caccgacgac ctgtggggac
     8941 ggtaatttgc cggttttcag aatggtgctc agcacatctt tttgttctgc cagccatagc
     9001 gaaagggctt cacgttgctc gtcttccatt tgcactggag aatttgtcag ccaggtgtca
     9061 agcagatccg ccgtatcaag cattttatca taagcatcgg cttccttttt gctggtaaac
     9121 gacattttct cttcgccctc acgaatgact acgtatttaa cttcaaccgc catttgcagc
     9181 ctctcataat aactgtgatt ttatacagta tatttctttt cggttgagaa atcaacatca
     9241 gcaataaaga cacacgcaaa cgttttcgtt tatactgcgc gcggaattaa tcaggggata
     9301 ttcgttatga cgttattagg cactgcgctg cgtccggcag caactcgcgt gatgttatta
     9361 ggctccggtg aactgggtaa agaagtggca atcgagtgtc agcgtctcgg cgtagaggtg
     9421 attgccgtcg atcgctatgc cgacgcacca gccatgcatg tcgcgcatcg ctcccatgtc
     9481 attaatatgc ttgatggtga tgcattacgc cgtgtggttg aactggaaaa accacattat
     9541 atcgtgccgg agatcgaagc tattgccacc gatatgctga tccaacttga agaggaagga
     9601 ctgaatgttg tcccctgcgc tcgcgcaacg aaattaacga tgaatcgcga gggtatccgt
     9661 cgcctggcgg cagaacagct gcagctgccc acttccactt atcgttttgc cgatagcgaa
     9721 agccttttcc gcgaggcggt tgccgccatt ggctatccct gcattgtaaa accggtgatg
     9781 agctcttccg gcaaggggca gacgtttatt cgctctgcag agcaacttgc tcaggcatgg
     9841 gaatacgctc agcaaggcgg tcgcgccgga gcgggccgcg taattgttga aggcgtcgtt
     9901 aagtttgact tcgaaattac cctgctaacc gtcagcgcgg tggatggcgt ccatttctgt
     9961 gcaccagtag gtcatcgcca ggaagatggc gactaccgtg aatcctggca accacagcaa
    10021 atgagcccgc ttgcccttga acgtgcgcag gagattgccc gtaaagtggt gctggcactg
    10081 ggcggttatg gtctgtttgg tgtcgagctg tttgtctgtg gtgatgaggt gattttcagt
    10141 gaggtctccc ctcgtccaca tgataccggg atggtgacgt taatttctca agatctctca
    10201 gagtttgccc tgcatgtacg tgccttcctc ggacttccgg ttggcgggat ccgccagtat
    10261 ggtcctgcag cttctgccgt tatcctgcca caactgacca gtcagaatgt cacgtttgat
    10321 aatgtgcaga atgccgtagg cgcaggtttg cagattcgtt tatttggtaa gccggcaatt
    10381 aatggcagcc gtcgtctggg ggtggcactg gctactgcag agagtgttgt tgacgccatt
    10441 aaacgcgcga agcacgccgc cggacaggta aaagtacagg gttaaacccg gcaaaaaaac
    10501 gctacaaaaa tgcccgatcc gcgatcgggc attttgactt ttacagctta gcgccttcta
    10561 cagcttcacg cgccagctta gtaatacggt cgtaatcgcc cgcttccagc gcatctgccg
    10621 gaaccagcca ggaaccaccg atgcacagca cgcttttcag cgccaggtag tcacggtagt
    10681 tagccggaga aataccacct gtcgggcaga aacggacctg ggagaacgga cccgcgatcg
    10741 cctgcagggc tttcacgccg ccgttagctt cagccgggaa gaatttgaac tctttcaaac
    10801 cgtagtccat acccagcatc agttcggaaa cagtgctgat ccccggaatc agaggaatag
    10861 tcccttcggt agcagctttc agcagcggct cggtcagacc cgggctaatt gcgaactgtg
    10921 cacccgcttc ggtgacttct gccagctgct gtggattcag caccgtaccg gcacccacaa
    10981 tcgcttcagg cacttctttg gcgatagcac ggatagcgtc aactgcacac tcggtacgca
    11041 gagtcacttc cagaacgcgc accccaccag caaccaacgc ttttgccatc ggcactgcgt
    11101 gttccagttt ttttaccacg ataaccggta caaccgggcc ggtggtcagg attgattctg
    11161 cacttgtttt ccagtttttc atcagagttt tctctcgcct gattacaaat ttgtcgtctt
    11221 aaaaagtgat acaggttgcg ccctgttcgg caccggacag tttttcacgc aaggcgctga
    11281 ataattcacg tcctgtcccc acgcgtgacg cgctcaggtc aggaatgtgc ggttcgcgag
    11341 cagccagttc cgcttcgtct accagcagcg tcagttcgcc tgtctgtcca ttcacacgaa
    11401 tgatgtcccc gtcgcgcact tttgccagca gcccgccatc gtaggcttct ggtgttacgt
    11461 ggatagctga cggcacttta cctgaagcgc cggagagtcg tccatcggta actaacgcaa
    11521 ttttgaaaca ccggtccaat aatacaccaa gtggcggcat gagtttatgt aattctggca
    11581 ttccgttcgc ttttggcccc tgatgacgga caacaacgac acaatcgcgg tccagcaaac
    11641 ccgcttcaaa ggccggcata acgtcatgct ggctttcaaa aacaaccgct ggcgcttcaa
    11701 tcacctggtt ctcaaccggc acggcagagg ttttcataac cgcacggccc aggttaccgc
    11761 ttaacacttt tgtcccacca tgatgagaga aaggttgttc gaaggaagcg atcacattgc
    11821 tgtcgagtga tttttccgcc ccttcccgcc agtccagttc accattattc agccatggtt
    11881 caagggtata acgagacaga ccaaagcctg ccaccgtatt gacatcttca tgcagcaggc
    11941 ctgctttgag cagttcacgc accagaaccg gtacgccacc tgccgcctgg aagtggttaa
    12001 tatcggccgg accgttcggg tagagacgtg ccatcagcgg tacaacatca gaaaggtcag
    12061 agaagtcatc ccagttaatc tgaataccgg ccgcgcgcgc catcgccacc aggtgcatgg
    12121 tgtggttagt ggaaccaccg gtcgccagca gtgcaacgat accgttcacc accactttct
    12181 catcgatcat cttaccgatc ggcatccatt cattaccatt accggtcatg cgtgtaacct
    12241 gacgcgcagc ggcagcggtc aaagcatcgc gcagcggaga atccggatga acaaaagaag
    12301 agcctggcaa ctgcatcccc ataaattcca ccaccatctg gttggtgttg gcagtaccgt
    12361 agaaagtaca tgttcccggc gcatggtaag acgcggcttc tgactccagt aaggccatgc
    12421 ggtccacttt accttcggca taaagctggc gaatacgcac tttttcttta tttggcaaac
    12481 cgcttgccat cggtccagac ggcacaaaca ccgcaggcaa atgaccaaac gacagggctg
    12541 ccatcgtcag acccgggaca atcttgtcgc acacaccgag gaacagagca ccatcaaaca
    12601 tgttatggga cagcccgacc gccgcagaca tcgctatcac ttcgcggctt agcagcgaca
    12661 attccattcc atcctgcccc tgggtgacac catcacacat cgccggaaca ccgcccgcaa
    12721 cctgaccaac cgcattcgct tcatgcaggg ctttacgaat gatttctgga tagtgttcat
    12781 aaggctggtg cgcggagagc atgtcgttat aggaggtgat gatggcgata ttgttacgca
    12841 acatgctttt caaagaggct ttgtcttctg gctggcaggc agcgaaaccg tgtgccaggt
    12901 taccgcatgc caactgcgaa cgatgaacgg tcgaagtttt cgcttgttct atccgggcga
    12961 gataagcaga gcgagtctcg cgcgaacgtt caatgattcg atttgttacg cgtaacaatt
    13021 gtggattcat aaaggctcct gaaattgagt tgtcagagca ggatgattca caacgcgttt
    13081 cattcagagg atttatgact gaaacgcctg taaccggagc tcatagggca aaaacgtttc
    13141 agtcagtgta ataaaaaaag cctcgtgggt gaatccgcac gaggcctgaa agtgtaaaaa
    13201 ttgttctaca atctgtgcaa gatcatgtta ccggtaaaat aaccataaag gataagcgca
    13261 gatattactc aaactcattc caggaacgac catcacgggt aatcatcgcc accgaggcaa
    13321 cgggtcccca ggttccggcc tgatacggtt tcggcgcatc attgtccatc gcccacgcct
    13381 cagtaatgga gtctacccat ttccaggctt cttccacttc gtcgcgacga acaaacagtg
    13441 cctgaatacc acgcatggtt tccagcagca aacgttcata ggcatccgcc agatgcgtct
    13501 gattaaaggt ttctgaatag ctcagatcca gcttggtgat ttgcaggtta tgtttgtggt
    13561 caaggccagg aactttattc agtacctgga tatccacgcc ttcatcaggt tgcagacgga
    13621 tagtcagttt attctgcggc agatcctgcc acgattcttt aaacagattc agttcaggtg
    13681 ttttgaaata gaccacgact tcagaacatt tggtcggcag acgtttacca gtacgcaggt
    13741 agaatggcac accggcccag cgccagttat caatgtcgac gcggatcgct acgaaggttt
    13801 ctgtattgct gctcttgttc gcgccctctt cttccagata tcccggcact tttttgccct
    13861 gggcgaagcc cgcagtatat tgcccgcgta cggttttttc gcgtacgttg gagcggtcga
    13921 tgcggcgcag agacttcagt actttcactt tttcatcgcg gatgctgtct gcgctcaggt
    13981 cagacggcgg agacatcgca atcatgcaga gaatttgcag caggtggttt tggatcatat
    14041 cgcgcatctg accggcttta tcaaaatagc cccagcgccc ttcgatcccc acttcttctg
    14101 ccacggtaat ctcaacatga tcaatggtgc gattgtccca gttattcaca aacagggagt
    14161 tagcaaaacg cagcgccaac aggttcagca ccgtttcttt accaagatag tggtcgatac
    14221 ggtaaacctg gcactcctcg aagtattcgc caacctgatc attgatttcc tgcgaggtcg
    14281 ccagcgacgt ccccagcggt ttctccatga ctacgcgtgc cggtttagca ttcagtttag
    14341 cctcgccaag ccctttgcaa attgcgccaa aagtgctggg cggcatggca aagtagttaa
    14401 tggtgatacg atttttttga tccagcatcg cgccgagacg gctgaatgca gcagtgtcat
    14461 tgacatcgag attacaaaaa tccagacgtg cactcagggt gtcccataaa ccttcatcaa
    14521 tggtttcttt catgaaagtt tcgagcgcct cgcggacaac tttggtatat gccgctttat
    14581 cccagtcagc acgccctacg ccgataatcc gggtgtccgg gttgagctga ccggcttttt
    14641 ccagttgata cagggaaggc agcaatttac gacgcgcaag gtcgcctttc gcgccgaaaa
    14701 tgaccaggtc acaggcctgg gctgtttgcg ttaccgccat gtcattctcc ttaagttaac
    14761 taacccggta cttaagccag ggtatacttg taattttctt acggtgcact gtactgcttt
    14821 tacgagcttg cgaaaactgt aaacgcttat ccacccgtgc gattacggta aaagcgcgca
    14881 aagtgcggca aaactgataa aaaaatcatc gttttccatt gggtaaaaat ctgacactga
    14941 tcatgttatg aaaaaaaata acaacttttt tatctgcttt tgtcattaac acagcacaca
    15001 ggcgtaatat ctgacaaaac tacatttcga tttctttcag tgcggaaatc gtcattaccc
    15061 gtgagtttct ttacatcatg aatatgctgg aaaaaatcca gtctcagctg gaacatttga
    15121 gcaaatcaga gcgcaaagtt gccgaggtca ttctggcttc gcccgataac gcgatccatt
    15181 cgagtattgc tgctctggcg ctggaagcca atgttagcga accgacggtg aatcgtttct
    15241 gtcgcagcat ggacacgcgc ggttttcctg attttaaact tcatctggca caaagtctgg
    15301 cgaatggcac tccctatgtt aatcgcaatg tcaatgaaga tgacagcgtt gaatcataca
    15361 cagggaaaat atttgagtcc gcaatggcaa cgcttgatca tgtccgtcat tcactggata
    15421 aatctgccat caaccgcgcc gtcgacttgc tcactcaggc aaaaaaaatc gcctttttcg
    15481 gattaggctc ttcagccgcc gttgcccacg atgcgatgaa taagttcttt cgttttaatg
    15541 ttccggtggt gtactccgat gatatcgtgc tgcaacgcat gagttgtatg aattgtagcg
    15601 acggagacgt ggtggtgctg atttctcaca ctggaagaac aaaaaatctg gtcgagctgg
    15661 cgcagctggc tcgcgaaaac gacgccatgg tgattgccct cacctctgcg ggtaccccgc
    15721 tcgcccggga agcaacgctg gcaattaccc tcgacgtacc ggaagatact gacatttata
    15781 tgcccatggt ttctcgactt gcacagctga ccgtgataga tgtgctggcg acaggattta
    15841 ctttgcgacg cggtgcaaaa ttcagagata acttgaagcg ggtcaaggaa gcgctgaagg
    15901 aatcgcgttt tgataagcag ttacttaatt taagtgacga tcgctaaaaa cgactgtcac
    15961 tgtcctaatc ttatacgaca tccgaatgag attaatttat cgccatcgcg gcgttatttc
    16021 attcggattt catgttcaag caacacctgg ttgtttcagt caacggagta ttacatgtcc
    16081 agaaggcttc gcagaacaaa aatcgttacc acgttaggcc cagcaacaga tcgcgataat
    16141 aatcttgaaa aagttatcgc ggcgggtgcc aacgttgtac gtatgaactt ttctcacggc
    16201 tcgcctgaag atcacaaaat gcgcgcggat aaagttcgtg agattgccgc aaaactgggg
    16261 cgtcatgtgg ctattctggg tgacctccag gggcccaaaa tccgtgtatc cacctttaaa
    16321 gaaggcaaag ttttcctcaa tattggggat aaattcctgc tcgacgccaa cctgggtaaa
    16381 ggtgaaggcg acaaagaaaa agtcggtatc gactacaaag gcctgcctgc tgacgttgtg
    16441 cctggcgaca tcctgctgct ggacgatggt cgcgttcagt taaaagtact ggaagttcag
    16501 ggcatgaaag tgttcaccga agtgaccgtc ggtggtcccc tctccaacaa taaaggtatc
    16561 aacaaacttg gcggcggttt gtcggctgaa gcgctgaccg aaaaagacaa agcagacatt
    16621 aagactgcgg cgttgattgg cgtagattac ctggctgtct ccttcccacg ctgcggcgaa
    16681 gatctgaact atgcccgtcg cctggcacgc gatgcaggat gtgatgcgaa aattgttgcc
    16741 aaggttgaac gtgcggaagc cgtttgcagc caggatgcaa tggatgacat catcctcgcc
    16801 tctgacgtgg taatggttgc acgcggcgac ctcggtgtgg aaattggcga cccggaactg
    16861 gtcggcattc agaaagcgtt gatccgtcgt gcgcgtcagc ttaaccgcgc ggtaatcacg
    16921 gcgacccaga tgatggagtc aatgattact aacccgatgc cgacgcgtgc agaagtcatg
    16981 gacgtagcaa acgccgttct ggatggtact gacgctgtga tgctgtctgc agaaactgcc
    17041 gctgggcagt atccgtcaga aactgttgca gccatggcgc gcgtttgcct gggtgcggaa
    17101 aaaatcccga gcatcaacgt ttctaaacac cgtctggacg ttcagttcga caatgtggaa
    17161 gaagctattg ccatgtcagc aatgtacgca gctaaccacc tgaaaggcgt tacggcgatt
    17221 attaccatga ccgaatcggg tcgtaccgcg ctgatgacct cccgtatcag ctctggtcta
    17281 ccaattttcg ccatgtcgcg ccatgaacgt acgctgaacc tgactgctct ctatcgtggc
    17341 gttacgccgg tgcactttga tagcgctaat gacggcgtag cagctgccag cgaagcggtt
    17401 aatctgctgc gcgataaagg ttacttgatg tctggtgacc tggtgattgt cacccagggc
    17461 gacgtgatga gtaccgtggg ttctactaat accacgcgta ttttaacggt agagtaatct
    17521 cacctgcccc ggccgctgat gaccggggta tttagcaaca cataaattag gactactcat
    17581 tcaatccaat cataatcaca gccccctgta aaatgaatac agggatagcc tgaatggaga
    17641 tctaaatagc ttgcaagacg tcgcataccc gcttcctctg caacactata tccaataact
    17701 gccatcggta agccactatc tttggagtat tgtgtctgca cccatgtttt aattgaatcg
    17761 ttaatcgtaa tataataatc agcctgatat tccatatatt gtattggatc acaaaaacag
    17821 cctgccccaa tcccaatagt tctaactatt ctggctctat ctccataaaa ttggatccca
    17881 ggaagattat aaattttcaa atttttggca aaacgtttgg taatattaat ggcactgtca
    17941 ggttctatag catatacatg gtacatatca tcagaagcaa caatatttgt aacatccagt
    18001 tctaactgct gagccaaagc cttagacatt ccgaacgtag gctctcgatc catgacatca
    18061 tggcaacgaa tgattaccaa attattttta tgcaaaaact cctttttttg ctcaattatt
    18121 tttaaataac tgagttcacc gcgcgtataa tccattattt ttctggccca tgaaaactca
    18181 agaacgtcac cctccagatc agcataagaa taaaatgtag gctcatgagt aataatgata
    18241 ttggcacctt caaaaacagc ctttcttaaa acatcaattg aggcaatcca gcaggtccca
    18301 attttattaa taagtgtcgc ctcagcacca aaaataattt tatcaacagt tggttgcgaa
    18361 acggcaacca ttgaatttaa aaaacaatta agatctgcgg ctgttttaat atgatgcatt
    18421 caacatcctt caccggtttc atcagcaata aaataatttc aacatttctg gaaaacgttt
    18481 tgcccatgca gactcagagt gggtatcacc tccccataga tgataatcta tagttacatt
    18541 gcgttgttta cggagactca cgcataattt ttccgcgcct gaaatataga cttcaggaaa
    18601 gtcttcgcgc ataccactgg tttccagcgt accgacatcg agatatacag taagatctgc
    18661 aaagatattg ttttcctgaa taaaattcag cattgcgctg tcattgaacc atagtgccgg
    18721 cgaaaaaacg ccaacttttg aaaaaacgtc tagacgaaga attgcggcaa acaaactgat
    18781 gtacgcaccc atagaactac ctgctatcgc tgtattatta cgatctggga gagttcgaaa
    18841 attgctgtca atatagttct tgagcgtatt cacaataaaa tcaacataca acgcaccttt
    18901 tcctccacta taatccacat cagggtccca gccaggtaag ggaaaagatg tgtttagcgg
    18961 ccatggagaa tactcatcca tacggctgaa tttaccatga gacgatgcat tatcaatccc
    19021 cacaatgata acaccgtcgt attgggaatt tgggaaaaaa gcatctaccg cctcaggtat
    19081 atcccataca tgtggaccaa aagatacaga tttatcaaat aaattttgcc catcgtgcat
    19141 atacaataca gggtaactga catcagattc ataataggac gcaggtagta atattcttat
    19201 ttcgcgacta cttgccaatt gtggcatcgg aaaggattgc aacacatgat atcgattccc
    19261 atagttctca atatcgttca taatttaaat taacctcaat aaacaaaaga aatattacat
    19321 ctaagcactc ccctaccaca ttatatatgg gagcacttag aaatgactaa tatctatatt
    19381 tttatcttaa aaagtttttt cgcattggta aacata
//
LOCUS       DAZFJF010000088        19366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFJF010000088 DAZFJF010000000
VERSION     DAZFJF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.088"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(106..1107)
                     /locus_tag="ACHKAU_003396"
     CDS             complement(106..1107)
                     /locus_tag="ACHKAU_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5532392.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPQKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(1113..1460)
                     /locus_tag="ACHKAU_003397"
     CDS             complement(1113..1460)
                     /locus_tag="ACHKAU_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFO5532393.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(1490..2140)
                     /locus_tag="ACHKAU_003398"
     CDS             complement(1490..2140)
                     /locus_tag="ACHKAU_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532394.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKTLVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSALPEVRLRASELIRLYPKEIIFFICVGVLVGGAISRVFFNL"
     gene            complement(2156..2560)
                     /locus_tag="ACHKAU_003399"
     CDS             complement(2156..2560)
                     /locus_tag="ACHKAU_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5532395.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(2650..2787)
                     /locus_tag="ACHKAU_003400"
     CDS             complement(2650..2787)
                     /locus_tag="ACHKAU_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532396.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            2859..3062
                     /locus_tag="ACHKAU_003401"
     CDS             2859..3062
                     /locus_tag="ACHKAU_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5532397.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            3084..3434
                     /locus_tag="ACHKAU_003402"
     CDS             3084..3434
                     /locus_tag="ACHKAU_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO5532398.1"
                     /translation="MKKNTNNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHTWLIEKAGIVVHRCPHNPFTGNRIFALSSGDNQFGQDFTLYEALRTVD
                     RLLRGQSFIKQAAL"
     gene            3445..3723
                     /locus_tag="ACHKAU_003403"
     CDS             3445..3723
                     /locus_tag="ACHKAU_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532399.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQIIREIISEAEKSRQVNGDEPYIEIICMDSEAEIQKAD"
     gene            3735..3977
                     /locus_tag="ACHKAU_003404"
     CDS             3735..3977
                     /locus_tag="ACHKAU_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO5532400.1"
                     /translation="MDKEYKTLINKALERFYFRLSASGAHSERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            3974..4087
                     /locus_tag="ACHKAU_003405"
     CDS             3974..4087
                     /locus_tag="ACHKAU_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532401.1"
                     /translation="MSIFISWIVLIISVVCAIGIMRIINSVKKIERFFTGE"
     gene            4174..4377
                     /locus_tag="ACHKAU_003406"
     CDS             4174..4377
                     /locus_tag="ACHKAU_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532402.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQDPRK"
     gene            4701..5090
                     /locus_tag="ACHKAU_003407"
     CDS             4701..5090
                     /locus_tag="ACHKAU_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFO5532403.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASIFAAMLAHRTAGGCAS
                     LSEYSAEFNRLSALFSQEWQRVTGYQP"
     gene            5087..7927
                     /locus_tag="ACHKAU_003408"
     CDS             5087..7927
                     /locus_tag="ACHKAU_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5532404.1"
                     /translation="MSAFAWSWNEPRPAIDPARFTERRQETETDLQRAIRYYLEANKR
                     AQEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDSRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSGTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPIHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDTIVSHTRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSTRENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRS
                     LRDKQAKNSSDEMQNWTQSRKQPRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLE
                     QLGASDPEAAAHLASTLQQDQNTMKTPVTVLSDMLRAIRDAVHAQRISETTERAHRKA
                     DLLRGSLTSGNKKQTETGLTNPVNEQKTCRDI"
     gene            8004..8963
                     /locus_tag="ACHKAU_003409"
     CDS             8004..8963
                     /locus_tag="ACHKAU_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5532405.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNIIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            8968..9279
                     /locus_tag="ACHKAU_003410"
     CDS             8968..9279
                     /locus_tag="ACHKAU_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5532406.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            9343..9933
                     /locus_tag="ACHKAU_003411"
     CDS             9343..9933
                     /locus_tag="ACHKAU_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5532407.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKYAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNRGWFSPLTTDKNNT"
     gene            complement(10423..11469)
                     /locus_tag="ACHKAU_003412"
     CDS             complement(10423..11469)
                     /locus_tag="ACHKAU_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5532408.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(11469..13220)
                     /locus_tag="ACHKAU_003413"
     CDS             complement(11469..13220)
                     /locus_tag="ACHKAU_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO5532409.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATVTGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            13375..14211
                     /locus_tag="ACHKAU_003414"
     CDS             13375..14211
                     /locus_tag="ACHKAU_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO5532410.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLIAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QVDIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            14235..15287
                     /locus_tag="ACHKAU_003415"
     CDS             14235..15287
                     /locus_tag="ACHKAU_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO5532411.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILPDTTGPIARTNSSYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIGRQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            15333..16133
                     /gene="gpM"
                     /locus_tag="ACHKAU_003416"
     CDS             15333..16133
                     /gene="gpM"
                     /locus_tag="ACHKAU_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO5532412.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     VANQDIPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKNKKTTRKPATRKTTATQKAKRG"
     gene            16235..16729
                     /locus_tag="ACHKAU_003417"
     CDS             16235..16729
                     /locus_tag="ACHKAU_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO5532413.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            16729..16929
                     /locus_tag="ACHKAU_003418"
     CDS             16729..16929
                     /locus_tag="ACHKAU_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO5532414.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTRGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            16932..17255
                     /locus_tag="ACHKAU_003419"
     CDS             16932..17255
                     /locus_tag="ACHKAU_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5532415.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            17252..17644
                     /locus_tag="ACHKAU_003420"
     CDS             17252..17644
                     /locus_tag="ACHKAU_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO5532416.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            17641..18048
                     /locus_tag="ACHKAU_003421"
     CDS             17641..18048
                     /locus_tag="ACHKAU_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO5532417.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASTLKST
                     VDTLNIISAGVQDMQQVLAQLRADNQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHAGNASPLP"
     gene            18187..>19366
                     /locus_tag="ACHKAU_003422"
     CDS             18187..>19366
                     /locus_tag="ACHKAU_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5532418.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSVLNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFAAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRSIIPDRMATAILNVAGR
                     T"
BASE COUNT         5074 a         5194 c         4871 g         4227 t
ORIGIN      
        1 actatcaacc accatttata aatcataacc ttatgatttt attaactttg aaaatggctc
       61 tatacaacgc catttaaatc tattgtcact ttttatcgcc actttttacc ctctattgtc
      121 aaaagggttc aattcaacag cagcctctaa atgacctgga gcaaaatgcg cataacgcat
      181 agtcattttt atatcgctat gccccagtat tttttgcaac acaagaatat ttccgccccg
      241 catcataaaa tgactggcaa acgtgtgacg tagcacatga gttaattgcc catcaggaag
      301 ctcgatcttc gctctcttaa ttgcagcgtc aaaagcctca taacatggtg aaaatagcgc
      361 tcctcgtttt tgaggaagca tagcctgcaa ttgaggtgaa atcggtacag tgcggttctt
      421 ctttccttta gttttaacaa atgtgattcg accgggcagt acttgagatt gctttaatcc
      481 ttcagcttca ctccaccgag caccagtcgc aagcccaata cggacaacaa cccccaaatc
      541 tttattccgt gactcatcac acgcaatcag aaggcgttca atctcatcta catacagaaa
      601 cgccagttcc ttttcttcct cacgaaactt gcgaatacca gtcagggggt tttcaccaga
      661 ccactcccca aggcgcttca gttcggcaaa aacagcatgt agatatgact gctcgcgatt
      721 aacggttgct tcactaagtt ttttcttccc cttgggattc cattctcctg atagccttct
      781 ttcccgataa gtagcaaaca tatttttgtc aaactgagaa gcaaatggat cccccagcct
      841 ggaacaaatc gcctcaagtt tgactttgcg ctctgcacca gaggacaagg ttttaccgta
      901 catctcaaac caacgagcaa tcaactcaga aagacgagga ccagaaccat cttgaaactc
      961 gtctccaact ctactattca ttaaacggcg ctcataagag agcgcctcac tttttgtcgc
     1021 aaactgttta cgaatgcgtt ttcccgatgc cccgtaggga taacattcgc aaagccattt
     1081 acctgatgga atcttacgaa ccgacatttt agttacttat cacataaatc aaatgcagcc
     1141 ttagtgacat cccccagact cttttttaac cctggggcgg catcattatc tagccaaaat
     1201 ggattattgt tatctaacgg taacgcacca aatgttttac cttttattcg agctaaacct
     1261 gtaagcgcat ataacttatt atcgtcaaaa ttcatcacat aaggattacc atcaagacac
     1321 tgtaattgaa cctcatcagt attaaatggc cataccccat tgaaactctc acgttcaata
     1381 gttttaaaag gcattgcgac ggcggaaaaa gaaaacatag ataaaaaagt aactaatagt
     1441 tgaacctttt ttactttcat atcattacct caatttagct caagtaagat tacaaattaa
     1501 aaaacaccct agaaatggca ccgcctacca aaacccctac gcagataaag aatattattt
     1561 cttttggata aagtcggatt aattctgaag cacgaagtcg aacttctggt aaggccgaac
     1621 tctctgtgtg acttgatgcc gattgttgct ctaaccacga caatgcagac tgtaactgag
     1681 aacgagtaag atcgtttaaa cgtcctgtac cgaaattgat atggcaatac cgcagaagtt
     1741 tttgtcgaag tccacagtct tcactgttac gtagtaataa acttacaaga gtcttacagg
     1801 catcatgatc tttacatcgc tcaagcattg catgcagaaa actctccgct gttttatatt
     1861 gatttactgt catatcatca ataccagcta caccaatctc cgcatgtact ttttgccaaa
     1921 taataaacgc ttcagtattg ctagcttctg caatagcagc aaccaagcta tttagctcct
     1981 tacgctgagc cttaagcaaa gggcgatcgt catcatcatt attcgaaggg attgcgatat
     2041 tgacggtatg agaaccatca tatcgctcta tctgaatatt cttttcgtga aaatcacgcc
     2101 cagcaacgcg attgtttgaa ccgtttgagt tgacggccat gtcacctccc tactatcacc
     2161 tacccttagt ttcgttatag tcacgaccag cgatacggtt attaccacca gaaatattta
     2221 actcacgtcc tgatggctga gtttcctttt cactgatcgc accttttaaa gccccgatca
     2281 ccgcgttttt cacatctaac gaagctgctc gaaagcgagt aatcaactcc tgctcatcat
     2341 cgttataagt ttcaggtgag tgaattccca acacaacata ctgaacatca aggccaaaac
     2401 gagacagcgc tgccaaatac gcagcatcag gaaagctatc tcctttctca tatctaagct
     2461 gagttagctt tttgactcca ccaatgtcgc tcatggcaac ttgactaagt cccaatcttt
     2521 ccctttcctc acgcaaccgc tgaccaatat catttttcat acaaaaacct tgacaggtat
     2581 cttttttgat accaaaatga tttcacgagc tattagatga tcacaatata ccactatgaa
     2641 acaagttctt cacgatacca gatcacgcat tccgcgtaac accgccacag gtccaagact
     2701 ggcacttcgg ctgtccctcg aggagcgagc cgtcattgat gaaatggcag ctaaagaaca
     2761 acgctcatcc tctaacatgg cgcgcatgat cttccttcgc ggcctagagc taacccagaa
     2821 agaacaaaac aaatcttcct gatcaggagg ctagtgggat gtcaggtata accatcaata
     2881 tcaatgtgaa tgccccctat gtatccctgc agaaatatgc agagataaca ggtatccctc
     2941 ttaatacatg caaaaagatg ttggctgacg gtcgaattat tatccgcccc aaacgcgcca
     3001 aaatggaaaa gcctgaagta aaccttgtgg cgatgttaaa agacgctttg gctaacagct
     3061 aaaacaatga acagagcatc atcatgaaaa aaaacactaa taatccatac tccaaatttc
     3121 gtaatggcgt agaacgccat gtacaccacg tcgctaccag tgcatcacgt agtaacagcc
     3181 gctataacct gaacgagacg cacgcaacac cagatgggca cgctgtaaaa caaatcggcg
     3241 aacacacctg gctgattgag aaagctggaa tcgtggttca cagatgccca cacaatccgt
     3301 ttaccggaaa ccgcattttt gcattaagca gcggcgataa tcagttcggg caggatttca
     3361 cattgtacga agcactacgc acagtcgatc gtctgcttcg cgggcaaagt tttattaaac
     3421 aggctgcttt ataacaggtg ctttatgact aaagaccatg cacaaggtgt atttatccgc
     3481 tttattgatt ttcgcggtga actgttatta cgcgcatcag ctattgacgg agtagttcca
     3541 tctgaaaaaa acgcagctac ctacgtttat ctgaacggta cacgcctgac tgtagagctt
     3601 ccgtaccaga tcattcgcga aataattagc gaagccgaaa aatctcgtca ggttaatggc
     3661 gatgaaccct atatcgagat tatctgcatg gattcagaag ctgaaattca gaaagcagat
     3721 taaagggtgt tgcgatggat aaagaatata aaactctcat caacaaagca cttgagcgtt
     3781 tttattttcg tttaagcgca tccggcgctc attcagaacg tgcagcccgt gactcattga
     3841 ccagggctat ccggagtctg tatgacgtag ctttttacgc tgacgatctg gatgcactta
     3901 atgaactttc tgagcttatc tgcgccgcag aatgcgggga gcatattgag ccgtacaaac
     3961 tgggaaatat cgcatgagta tatttatatc ctggattgtt cttattattt cggtggtttg
     4021 tgcaattggt attatgcgaa ttattaattc agtaaaaaag attgaacgct ttttcactgg
     4081 cgaataaccg cataaataaa accccagctt aaataaaaaa atgtgaacac aatctgcatt
     4141 cgcagaggta ttcacacaca ccaaggaggc gtaatggcaa ttaagcattt tcctgtcgtt
     4201 cgtttcacct ccagaggacg tgaatacgaa gtcgacgaac gtctgattac cacaatcgac
     4261 aaacaccgct cagaaaagga tgcacaccac atctatctca ctgacggcac ttacttttgc
     4321 gccactaatg tggcgcgggt gaatcttatc cgacaggtac aggatccacg caaatgagta
     4381 ggagaagaat cactcgcaga catcaccgaa cacacctgag ttcctcagca acgctaaagg
     4441 cacttattca aagcgagatc ggtgatttct tcgcgggagt tggctcacca ggcgaaccag
     4501 aaacaccaga agcgatgcag cgtgagctca tgatacgcat agataacact tttgatttct
     4561 tctacagcat gcacggaatt aaacagaaat gaacctcaag ccagcaataa ctactcgtag
     4621 aacgtcaatt cctgtaaccg accgcttctg agttttttgg caggaagcct tcgcacatct
     4681 gtagtaaaga gaattgcagc atgattgacg ctcatgactt cacaagatgg gtgcgcacac
     4741 aggacacccg tctggctccc gttcttcagg gattatttga tctctacatc cgtggtcgtg
     4801 acaacagagc acgcaccaca aaaccggaga atgcagacac cctttatttc acagtagacg
     4861 actgctaccg cgtggacttc acaccacacg ggctggcgtt gcactgcctg acaccacacg
     4921 gcgaatcact gctggcgtat tacgactccc cggcctccat atttgcggca atgctggcgc
     4981 atcgcactgc tggcgggtgt gcctcgctga gtgaatactc cgctgaattt aaccgccttt
     5041 ccgccctctt ctcacaggag tggcagcgcg tgacgggata ccagccatga gtgcgtttgc
     5101 atggagctgg aatgaaccac ggccagccat tgatccggcc agatttacgg agcgcaggca
     5161 ggaaactgaa accgacctgc aacgcgccat ccgttactac cttgaggcaa acaaaagggc
     5221 acaggaagaa caggaagcga aggaagaagc ctttttcgca caatccaccg tgggtaaaaa
     5281 actcatggca tcccttgagg aagccggaca gcgtgaaaag ctggcacaaa gcatcatcag
     5341 caagcgtcag gcaacagaac aagacccggt ggcccgtgct tttgccacac tgaaggtgct
     5401 tcccgtttat ctgcgtgaac ctctgagccg ccacctctct tttctgcgca agaagcagga
     5461 agccgatcgt cagaaaggca aaaagagctg gcaggctgaa cgctacgcgc gcggaaccct
     5521 gcgcaaaata ttcgaacgtc tggaccgcac cgacagccgc tggctgacac cgggctatcg
     5581 ctcccttgcc ggacgcgaac gcctggacga tttgctttac ctgccgcagc tcaacaaaca
     5641 ccagatacag acgctggcca ccatgacggc ggcgatgttc agtggcactt ttgaaaaact
     5701 ctgcgatggc tttggcgcga ccgatggcga gctgaccatg gatgtaacgc tgaaggcgta
     5761 tcagatgctg gcccgcatgg cgttacacct gcacgctatg cctccacatt atgacgctct
     5821 gacaacagac aaagaccgga ggaatgaacc ggacacggag ctgctgccgg gcgcaatcct
     5881 tcgcctgacc tgtgcggaat ggtggaaacg caaactgtgg ctgttacgtt gcgagtggag
     5941 agaagaacaa ctccgcgccg cctgtctggt ttccagaaaa acatcaccct atctgagcca
     6001 ggacgcgtta agcgagtttc gcgcacagcg cgagaaaaca cgcgatttcc tgaaaagttt
     6061 catgctggaa aacgaagacg ggttcacgat tgatctcgag acagtgtatt acgcgggagt
     6121 aagtaacccg attcaccgta aggcagaaat gatggccacc atgaaggggc tggaacttct
     6181 ggccgaagcc cgtggcgaca gagcggtgtt tctgactgtc acctgcccgt caaaatacca
     6241 cgccacaaca gagaacggtc atccgaatcc caaatggaac ggggccacca tgcgcgactc
     6301 cagcgattac ctggttaaca cgttttttgc ggcggtccgc aagaaactga accgcgacgg
     6361 cctgcgctgg tatggcatcc gcacggtgga gcctcaccat gacggcaccg tgcactggca
     6421 tatgatggtc tttgctcatc cggaagaaat cgacaccatt gtgtcccaca cccgcgatat
     6481 tgccattcag gaagaccgcc acgagctggg caatgatatt actccgcgct ttaaggtgga
     6541 gtatgtcgac ggctcaaaag gcacgccaac cagctacatc gccacctaca tcggaaagaa
     6601 cctggacagc cgcgccgtgg atggcatcga cccgaaaacg ggcaagccac gcgttgacca
     6661 cgaaaccgga aaatcaatgg ccgagagcgt ggaacgcgcc atcggctggg cacgccttca
     6721 ccgggtccgc cagttccagt tctttggcat cccctcccgt caggtatggc gtgaactccg
     6781 ccgccttgcc agccagatgg cacgcaaccc ggaaggccca caacggctga aggatgatgc
     6841 aatggatgcg gtactcgctg ccgctgatgc cgggtgtttt gccacctaca ttgagaaaca
     6901 gggcggcgta cttgttccac gcaaagacta cctgattcgc accgcctacg acctcgcaga
     6961 tgagctgaac gattacggcg aacagagcgt acagatttac gggatctggt caccactcat
     7021 cggggaatcc tcccgtgtgt gcacgcaccc ggataactgg aagctggtaa gacgtaaacc
     7081 ggaagcggaa gacagcaccc gcgaaaatgg ttttgacctt cagggcggcc ctgccgcccc
     7141 ttggactcgt ggcaataact gtccccgtgt acaggaaacg gacaacaacg ggacagaaca
     7201 gccggaagaa aggccagcac cgtggccgca gcttcctgac ggcgttgacg tgaatgaatg
     7261 gatgcgctca ctgaaacggc acgaacgccg ggcgctgatg cgttcgctgc gtgacaaaca
     7321 ggcaaaaaac agcagtgatg aaatgcagaa ctggacacag agccgcaaac agccacggcc
     7381 tttgcctgat aatcacgagt tactcgctaa agaatggcgg gagtctgctg aatctctcgg
     7441 cctgcatatc ggtgaacagc agatgcagca cctgttacgg ggcggcagtc tgtacgttga
     7501 cggcagcatc attgcaccgc agggatttga aattgtacgc aaaccagata cccgcccgga
     7561 cagccgaatc acgcaactct ggcagcgcct gagccgtaat cacggcgtaa gcagcacgga
     7621 gatccgccat aacccggtcg ccagctatct ggaacagctg ggggcatcag accccgaagc
     7681 cgccgcacac ctggcatcca cacttcagca ggaccagaac accatgaaaa cccccgttac
     7741 cgtgctttct gacatgctgc gcgccatccg tgacgcagtg cacgcacaga gaatcagtga
     7801 aaccactgaa cgcgcccacc gcaaagcaga cctgctgcgg ggtagcctga ccagtggaaa
     7861 caaaaaacag acagaaacgg gactcacaaa tcccgtaaat gagcaaaaaa cgtgccgcga
     7921 tatatgaagc gcgcacaaaa caggcaaaag cgggatttaa aaatcctgta accgattaat
     7981 taatcaacac aaggaaaagc gacatgaaaa tttgtatcga cgacggctcc accaacatca
     8041 agctggcatg gactgagaac ggcgaacgcc gcaacgccat cagcccgaac agcttcaagt
     8101 cggaatggtc tgcgccgttc ggtggcacgc agcccgcgaa ctacatgctt gatggcgtgc
     8161 gctatggttt tgatccggtc agcgatcgct ttgtccagac gaccgacacg caataccagt
     8221 acagcgatgt gaatataatt gccattcatc acgcgctggt caaatcaggc atcacaccac
     8281 aggaagtgga tgtggttgtc accctgccac tgagcgagta tttcgataca aacgcacagc
     8341 cggacatggc caacatcaac cgcaaaaaag cgaacgtcat gcgcccggtg gagtaccaga
     8401 acggcgaagc attcactatc cgtaacgtac gggttatgcc tgaatccatt ccggctggct
     8461 ttaaggcact ggctgacatg agtccgtttg aatccctgct gattgtggat ttgggcggaa
     8521 ccactctgga tgtggcaaag gttcaggggc aactggcagg tatcagccag gtgttttgcg
     8581 atccacacgt gggcgtttct ctgatggccg atgccgtact gtcggtgatg gccactaacg
     8641 gtatgcgcac cagtcaccac atcgccaata ccattatcga acatcgccat gatgaagcct
     8701 ggctgcgcca gcacatccac aatgacgcgc attacgccag cctgatggcg gttattcgtg
     8761 aaaaggaaga aacactgaaa caacgcgtga tccgcgcgct ggcgggtttt tcgggttacg
     8821 ggcgggtgat ggttgtcggt ggaggggcgg agattgtggc acccgctatc cgcgaagcct
     8881 gcggagttaa tgcgactttc atcgcggacg gggtgccaca gtttgctctg gttaatgggc
     8941 tgtacgcaat ggacaaggag taaaccaatg acgactccaa ccagacggat aagtttctat
     9001 ctgaagccca ccgccgtcaa gaacgaagga gaagcatgcg cctggctgga cagccttaca
     9061 ccagaagccc gcaaaagcgg ccaacgcgtg gcttttctgg ccgggctggc acttctgaaa
     9121 atgaatccgg cagaggctta tcgactggct gcatgggctg gtgatgaagc gttatcagtg
     9181 acacaaacca ggacagaacg ccccgcatca cagccagtat caaccgcaca gataaccagt
     9241 cagatggccg gaaatatccg ggcgttattt cctgaataac aaaacatcag ggcgagttcg
     9301 ccctgctctc cacctcagaa cataaacaag gagaacgact taatgagcga aatcaactat
     9361 caggcattac gtgaacgtta ttcacctgtg ccagtaccga aatgccctat ttgcggcgag
     9421 gaaatgtcaa ttcagcgaat atctggagca caggttgttt atgcctgctc cggttatggt
     9481 gatgatggag atttcaaaat tggccgaact cttgccgacg aacattatga aaaatcacgc
     9541 gtaacagtgt tggatgtcgg ggatcctgaa gtattggcgc tacttgattg gctggaaacc
     9601 aaagacaacc gaatcgctga actggaaaaa atcgccactg actatgcact taaattccag
     9661 aaagcacagg acgcattaaa gtatgccgct ttgctgcata gcaggacggc gcaacaaaca
     9721 aataattttg cagtatcgct tccggacata agcgaatatt tcattaatga cgtatttcag
     9781 cccttgcgat acgagcggga tgttgaaaga gccatcataa aggctggcgg aaaagcattg
     9841 tggcaggaga aacacgagga cagaacgcat cagtcctgcg atgtaaatcg tggatggttt
     9901 agcccactga cgacagataa aaataacacc tgatccccct caaaccaggg cgataatcgc
     9961 gcatcgccct gctgcacaat agtgcacaag tttgcacaat ttttttgaac gactttttac
    10021 ccttccggcc cgcatggcgg ctggatccgt caaggatccg tgcgtgcaca aaaaaacgcg
    10081 ctttttctgc gcgcaggtga cgggggaaca gcccgcgttt cagggggtaa atagcatccc
    10141 ctgaacgatg tcgcagcgac acaacagaat ggctgtattt ctcacgctga gcgtgaaaaa
    10201 gacgtgaggg cttttgattt gatgggtggc agataaggcc gtcaaaatcg cactgaggcg
    10261 gcgagaacat gcagtcaacg cggtgggatt gcgtaagagt ctgaccgccg atgatgacaa
    10321 taagcagaaa agcgtcgtga aattatctga ttgatacagg agctggagag tcggggcata
    10381 aattttttat gccccggcga agcagcagac aagcgaagcg cgtcaggatg tgggctgggt
    10441 atctagcagt gcgtaagggt taaagcggat cacctcttcg ccaagccagt cattgatgtg
    10501 cttcatggcc tccataacgg gcatcagctc gttaattgcg taaacccgcg cggccttctc
    10561 cacatcacca aacgcacttt tttcgcccgg catcgccccc atcagttgcg gcggaacgcg
    10621 gtgcgcagcc agcacatcat cacgggatgc cgccttaaca ttcatgaact catcctttgc
    10681 agtgatctgc tggaacggca aaatttgcac accttctttg cccccgttgg gcgcatggat
    10741 gagtacgttt ttaaacgcac caccaccacg cgcgccctgt aacgtttctt tcagggagtc
    10801 catgctttcg cggtttacct gcgctgcacc gatgtagatg atgcacccgg cgtgggatcc
    10861 gttgtcgtag tacagttttc tgaacatgtc cgccgaatga gaaaggctgg ccgagagtaa
    10921 tgcgccaaga tattccggca tgccgtagat ttcctggtta atatccggat tcatcaggtg
    10981 gcacactttg ccagggcgaa actggaacgc gtccttgcca tcctgcacat accaccatga
    11041 ttcaagatcg cttccgcgtc gcatgtattt cgccagggcg tgccgtaatt taagcggttc
    11101 gccgagcata ttgcttctaa gctcaaggaa tgcgttaccg aacacaaacc agtccagcgc
    11161 cagcgccgag aaatcctgcc gggaaagcag cgggtgcggg atgtagcaac cgagtaatac
    11221 attgcgctta aagtaaagcg cagactgatg ccaggacgtt tgccgggctg ctcttgccag
    11281 accgtaccag tccaccgggg tttcatacca ccgtccgtta tcagcacagt acatattgtc
    11341 cagcaggtca tgcccggtca ggcgataagg accatcaaat gtgaatgcac tgagcgatga
    11401 ttctttcctg agcgcatcag cgagatcaat gcgtgaactc atgcgcactt ttttattttt
    11461 tctgctcatc agaactccat aaccgtgaaa cgctcgtttt ctccttcgcc gccaatcggt
    11521 tcgttaatga cagcaagcat ggttgcccac gcaaggtcgc cgtggctgat ccccctcgcg
    11581 cggtccgttt cgtaagtgat aaagccgccc ggtgttttca ccttacgcac ggcgttaaag
    11641 gccgcgacca gctcgcgttc ggcgcgatcg tattcccacc gcccggcacg cattatttgc
    11701 agcattttca gtaccagcga ccgttttgat gacagcgtga aggtgtacgg aatagcagca
    11761 gggaaaaacc gtttcactat ctgataaaca gcctccccgt tcccgcccgt cacatcaatg
    11821 ccgatgtgtt ccacgttgta gcgatacgtg aactcttcaa tgactctggc ctgttcttca
    11881 aactccagcc cctgaacgcg tcgcgtctcc accgttcgaa aacggccacc aggaacagcc
    11941 ggaggaacca ccacggacac agcgccgctg tcgccgttgc cactactgcc gtttgcgtca
    12001 tacccaatcc ataccggacg attccccatc gggcggggag caaaaggttt ccagtctttc
    12061 cagtcgtcgt atccgtcaac accgcagcca atcaggatat tcaggttaaa tgccgattcc
    12121 ccttcgcgga caaactcaca catatagaga ttgaggaact cgtcttcggt gttttcatca
    12181 cgaatttcgt cgatatcggt gtgtttccag ccgtgattga ccacatcttc cagcgtgaca
    12241 atttgccgcc acgtccggtc agggcagata agcccgttat gcagcgtttt ccagtccaca
    12301 gaaaaacgct ggcgtttatg cgtggccttt ttctcgttcc agcggtcgcc gttccagtag
    12361 gcgtatgcct cgtgcgtttc ggtggatggc gtggagaagt aggtgcgccg cagtccgctg
    12421 agggttgcca tagcgccagc caccttgcgc agttcagcaa agcgactgac ccagaaaaat
    12481 tcatcaaaat aaaaattgcc cgtataggac tgtgccgacg cagcagaagt gccgagaaaa
    12541 tgcagctctg cgccgttgga gaggatgatt ttatcgcccc ctttcagctc cacatcaact
    12601 tcagccgcgg ccttctgaat aatgctttta aactggaacg cctgacgacg cgacgcagac
    12661 aaaaaaatct ggttacgctg gtaaggttgc gccacatcgt cacgcagcgc catcagcaga
    12721 gcttcctgtg caaaatacca ggtcgcccca atctgtcggg atttcaggat catcctgtta
    12781 cgtatcccgg cttccctgca aagggtcagg gagtcaaacc agccccgctg atgccactcc
    12841 agcctgctga tgattttttc ccgcagtgcg gcaatctgtt ccggcgtgaa atgatttttg
    12901 agttttttcg cccggccttt ctttcctgtg accgtcgcat ccggctggcc atcatgcagt
    12961 tttttaagct gccgggtcag caggtctatt tccttaaagt caccgcctgt tttattctgt
    13021 ttttcagtaa gctggatgag gcgcgcatcg atggactgcg tgacacgctg cacgggtggc
    13081 gtttcatccc actggtcgcg ttttttccac gcataaatcg tgttcgggtt tattcccatc
    13141 agacgtgata tttctgcggg cggataaccc tgccagtaaa gttgccgcgc acgctggcgc
    13201 acaaaagcgt cctgaatcat tgctccccct gagtaattac aggaagatta cccgcgcgcg
    13261 aaactgttct ccttaacccc ctgttctggc cgttttctta caacaaaatc cctttgtatc
    13321 agcctgttac gctttgccat catgactgaa gaaccagtca gaggggcaaa aactatggct
    13381 aatgaaaaaa agacatcccg caaaaagttt cgcgtggctg tctccggatc aactgttgat
    13441 ggccgcgaaa tcagcccggt acatctgcgt gaagccgccg agaacttcaa cccggatgtt
    13501 tacgctgccc gcgtgaacgt tgagcactat ctctcgccat gcccgtcaag cgaattttcc
    13561 gcaatgggcg atgtcaccgc gctgagtacg gaagatatta cggaaggccc gctggccgga
    13621 cgtactgcgc tgtatgcaga aatcgaaccg accgagcgca tgaagcagct tatcgctgac
    13681 ggcaagaaaa tctattccag tatcgaactg cacccgcagt tctccgttaa cgggcgtgcc
    13741 tatctggtcg ggctggcgat gaccgacacc ccggcaagcc tgggcactga gcgcctgaaa
    13801 ttcacggcac agcagcgtca ggcggtgatg acgttcaaca gtatccaggg tgaagcaccg
    13861 cttatctccg aagccatcga gtctgaaatc atcgaaatgg cagaacaacg ccaggaagaa
    13921 ggcacccagt ggtttaaccg cgtaatgggg attattggtc gtggccgcaa agcggatgac
    13981 gccagtttct cccgtattca ggaagcggtg gaaggcgttg caacatcaca ggtcgacatt
    14041 atcgaccgtt ttaatgtgct ggaaacccgc catcagcagg accgccagaa aatcacgtca
    14101 ctgaccacag agctgacagc actgaaggaa aaactgcgca cgcaggacgg cgatccgcag
    14161 aaccgcttca ccgcaacggg cgcagcctcc gaccagctgg ctgacttctg ataagacaaa
    14221 ggagcaaatt ttttatgaat ctggtgatgt cagatattac ccgcaacaag ctgggttgct
    14281 atatggcgca gcaggcgtcg cttaacaata tcccggtatc tgcactggta tcgcgattta
    14341 ccgtggaacc cgcggtgcag cagcgttttg aaaacgcctc aaaggaaagt accgaattta
    14401 cgaaaagaat taacgtgatc ggcgtgaccg accagaaagg cgaaaaaatc ctcccggaca
    14461 ccaccgggcc aattgcgcgc acgaatagca gttatgacgg catcaaacgc cgtaacccga
    14521 ataacgtgat cgatatgaag tctcgtcagt accagtgcga acaggtgaac tacgatacct
    14581 ttatttcgta cccacagctt gatacctggg cggcccacag cgattttcag tcccgtatca
    14641 gtacacagat cggccggcag gtagcgcttg atcgcatcat gattggcttt aacggcacat
    14701 cccacgccta cgagtctgat tttcacacca acaagctgct tcaggacgtt aacgtgggct
    14761 ggctggagca catcagaacc gatgccagcg agcgcgtaat gaatgacgtg acgctgacct
    14821 cccgcaacat ggacaacact gtggcgcacg cgggtaagta tgcgaatgcc gatgctctgg
    14881 tacaggatgc gcgctcatcc ctgctggatg aatggcacaa ggaagctgac gacctcgtgg
    14941 tgattatggg gcgcaacctg tttaactcgc tgcgtctgcc cgtgctgaac agcatcagcg
    15001 gccagaatcc caatgcggaa ttacttgccg ggcagctcat cctgtcatcg cgcaccattg
    15061 gcgggctggg cgtgttcctt gcgccgttct tcccggatgc aacgatgctt atcacctcgt
    15121 tcaacaacct gtcgatttac tggcagaaag gttcaatgcg tcgtctgatg aaagacgagc
    15181 cggaatacaa ccgcatcgcc acctaccagt ccatcaatga cgcttatgtc gttgaagact
    15241 atggcaagtg cgcgatggtc actggcctga agttcgccga cagctaatca actcacggcg
    15301 ggcatcatgc ccgcctgtaa cggagagaaa aaatgattac tcctgcacaa caacactggc
    15361 agaacgtgat ggcacagcgc gcaggccggg cgaatgaagg tgtggaccac gccgcgtgta
    15421 ccgcgcatga agaagtgctg tatcgtctgc gtctggcaca ggccaggcta aaggccatac
    15481 aggccagaag cgcgaaagcc gccatcaaaa aagagttgtt gccggacttt tccggctgga
    15541 ttgagggaac gctggaggct gacggcgggc aacaggacga agtaattgcc acgctgatgg
    15601 tgtgggcgat tgactgcggc gatcttccgc tggcgctgcg tattggtgca tatgtggtcc
    15661 gtcacaacct catcatgccg gataactttg gccgtactgc tgccacggta ctgaccgaag
    15721 aaatctgcaa cccggtactg acgcaggctg ggacggatgc cgacgcggat ttatccgcct
    15781 ttatcgaacc actggacaca ctttgggaaa ttgtcgccaa ccaggacatt ccggacgaag
    15841 tgcgcgccaa attatgcaag gcgtgtgcct ttgcccgtcg tggcctgacc gatgcagaca
    15901 acatggcctc atcactgaag ctgctgcgcg aagcgatgca cctgaacccg aacgcaggtg
    15961 tgaaacgcga gattgcaacc ctttcccgcg ccctgaaaaa agccgattcc gcagccgaac
    16021 cagaagacgc cagcgcacag caggcgcagg acgaaagcag caaaaataaa aagacaacgc
    16081 ggaagcctgc aacacgaaaa accaccgcga cgcagaaggc aaagcgcggt taacgactga
    16141 ccccgtcagc gggcggcgtg cgcggtgttc cggtttgact ccgtgaccgt ttacaccgcg
    16201 cacccaccgc ccgatttttt caggagtgaa ccccatgagt atggttgcca gaactaaccc
    16261 cggccccgca gaggacgaca tcaccgatac cgatgatggc gacactcgca tttcagcggg
    16321 tgcattctgg ccggatattg tgctgcgtga gctgcgtctg gcggtacgac tgccgggccg
    16381 cgtgaccacc tcccgcctgc tgcataccgc caccggggcc atagcacacg ttacccgcga
    16441 gctggaagca tggcagcagg aacaacaggc ggctggctat cagacgctgg ccgatgttcc
    16501 ggcccctgta attaacggag aaagcgtcaa tctctggcac tggcgcaatg ctgtttatac
    16561 cgccacacgc gccctgattc tggagcgtta ccgcgatgcg gacacaacgg acaagggcga
    16621 ccgccgggcg gacgcactgg atatacagac atcggatttg tggcgcgatg tgagctgggc
    16681 catctctgac attctgggac gaccgcgaat gtttgcggag ctgtgctgat gaaagtgaag
    16741 gcactggaag gcgacaccgt ggattcgctc tgtttccggt actacggcac gacacggggc
    16801 gtcaccgaaa aggtgctgga tgccaacccc ggactctgtc agcaggtatt tctggacgcc
    16861 gggcaggaag tggagatgcc ggagccggag aagaagaaac gagaaatgat tcagttgtgg
    16921 ggggagtagc agtgagcacc attcaaacag ggatcacaga gcaggttatt gcgtggctct
    16981 ttgaccacct gccaacggtg tatgcagtag gcgcggctgt cagcatttcc gcgctgatga
    17041 gtctttatga cggacgaaca ctggttcaga ccgtaacggg atcgctggcg tgcggcgttc
    17101 ttgccatggc cgtggccggg tcgctgcgct tcttcgggat ccctgaggat gcagtgacgt
    17161 tttttggtgc ctcaatcggt tttatgggcg cagagaaagc acgcgacaag gttattgcaa
    17221 tatttgatcg cagggtgaag gagaggaacg aatgagcaac acatttaaat tcagcagccg
    17281 aagcgaaaag aatttgcagg gtgtaaatcc tgatctggtg aaagtgaccc gacgggcgct
    17341 ggaaatctcg gaagtggatt ttggtatcac cgaaggattg cgcagtcgtt atcgtcagaa
    17401 gcagctcgtg accacaggca agagccagac catgaacagt cgccatctca cagggcatgc
    17461 cgtggatgtt gtggcttatg ttggcagcca ggtgtcatgg gaatggccgc tgtacgaaaa
    17521 aatcgcagca gcattcagac aggccagccg ggaactgaat attccggtgg aatggggcgg
    17581 cgactggaag accctgaaag acggaccgca ttttcagtta ccacacggag cctatccggc
    17641 atgaagctct ggcccacgct tggcgtcgct ttccttctga ttgccgcatg gggaacatcc
    17701 atgcgtctgt cgtggtcgct gggccgggag aacgccagaa acgaagcgca ggccagcacc
    17761 ctgaaaagta ccgtcgacac actgaatatc atcagcgccg gggtacagga tatgcagcag
    17821 gtgctggctc aactccgcgc ggacaatcag caacgcaatc aggacggaga ggtaagacgt
    17881 gaacagctac gcaacgatat tgcaaaagat gaatgcgccc acgctttgcc tgacgctcgt
    17941 tttactgaca ggttgcgcag gcacgcagaa cgcgccacgg ccagcgccgt cagtccggct
    18001 tataccgcag acgctgacca tgccggtaac gcctcccccc ttccctgacc aacccacatg
    18061 gggaaacctc ggaatatggg gcgaccgcct tctggatgca ctggaaacct gtaacgcgga
    18121 taaacgggcc attgctgaac tggataagag aatagccgaa ctgacacacc agacgggagt
    18181 aacacaatga ccagtaagaa ctttgcactg attacagcca tgacacaggc tgaactgact
    18241 caaaaggtga atgaacatct tgcgaaaggg tggcatcttc agggggagac gcgggttgcc
    18301 tacgaacccg gcaccccgtg gtatctaatg caggcaatgg tggccgatgg cactacagac
    18361 atctcacctg attcccccca gcacggcagc gtgccggagt ggtattacgt ggtggtactt
    18421 gctggtcaat ccaatgccat gtcatatggt gagggaatgc cgctgccgga ttcttacgat
    18481 gcgccccacc cacgcattaa gcaactggcc cgtcgcaaca cagtgactcc cggtggtaaa
    18541 gcatgcgcat ttaacgacat cattccggca gatcactgcc tgcatgatgt tcaggatatg
    18601 agcgtactga atcacccgaa tgcagacctg agcaaagggc agtatggctg tgtcggacag
    18661 ggcttgcata ttgccaaacg cctgttgcct tacattccac agaatgccgg gattttactg
    18721 gttccatgct gtcgtggtgg ttcggcattc acccagggcg cggaggggac attcagcgag
    18781 tccacaggag ccagtcagga ttcggctcgc tggggtgtgg gtaaaccttt atatcaggac
    18841 ctgattttgc gcacgaaggc cgcattgcag aaaaacccaa aaaacatgct gctggccgta
    18901 tgctggatgc agggcgaatt tgacatgagc gccgctacgt actcacagca acctccgctg
    18961 tttgcggcca tgctgaaaca gtttcgtgcg gacattaccg agtttaacac gcagtgtcat
    19021 ggaggcagag cggcaagtgt gccatggatt tgtggtgaca cgacgtatta ctggaaaaac
    19081 acctacggca cgcagtacga caccatttac ggggcgtaca aaaacaggga gagcgacaac
    19141 gttttctttg tgccattcct gaccgatggt agtggcaaca atacctccac caacgcacca
    19201 acggaagatc cggatgctgc aagtgaggga tattacggtt cggcatcccg aacgaacaaa
    19261 aactgggtat catcaaatcg ccagacgcat ttcagttcat gggcgcgcag gagcattatt
    19321 ccggatcgta tggcaaccgc tattctgaac gtagccggtc gcacct
//
LOCUS       DAZFJF010000089        18974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFJF010000089 DAZFJF010000000
VERSION     DAZFJF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.089"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            278..1006
                     /locus_tag="ACHKAU_003423"
     CDS             278..1006
                     /locus_tag="ACHKAU_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5532419.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            1006..5856
                     /gene="ehaG"
                     /locus_tag="ACHKAU_003424"
     CDS             1006..5856
                     /gene="ehaG"
                     /locus_tag="ACHKAU_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO5532420.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6225..7880
                     /gene="lldP"
                     /locus_tag="ACHKAU_003425"
     CDS             6225..7880
                     /gene="lldP"
                     /locus_tag="ACHKAU_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5532421.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7880..8656
                     /gene="lldR"
                     /locus_tag="ACHKAU_003426"
     CDS             7880..8656
                     /gene="lldR"
                     /locus_tag="ACHKAU_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO5532422.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8653..9843
                     /gene="lldD"
                     /locus_tag="ACHKAU_003427"
     CDS             8653..9843
                     /gene="lldD"
                     /locus_tag="ACHKAU_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO5532423.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10029..10502
                     /gene="trmL"
                     /locus_tag="ACHKAU_003428"
     CDS             10029..10502
                     /gene="trmL"
                     /locus_tag="ACHKAU_003428"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO5532424.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10555..11376)
                     /gene="cysE"
                     /locus_tag="ACHKAU_003429"
     CDS             complement(10555..11376)
                     /gene="cysE"
                     /locus_tag="ACHKAU_003429"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO5532425.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11456..12475)
                     /gene="gpsA"
                     /locus_tag="ACHKAU_003430"
     CDS             complement(11456..12475)
                     /gene="gpsA"
                     /locus_tag="ACHKAU_003430"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO5532426.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12475..12942)
                     /gene="secB"
                     /locus_tag="ACHKAU_003431"
     CDS             complement(12475..12942)
                     /gene="secB"
                     /locus_tag="ACHKAU_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO5532427.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13005..13256)
                     /gene="grxC"
                     /locus_tag="ACHKAU_003432"
     CDS             complement(13005..13256)
                     /gene="grxC"
                     /locus_tag="ACHKAU_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO5532428.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13398..13829)
                     /locus_tag="ACHKAU_003433"
     CDS             complement(13398..13829)
                     /locus_tag="ACHKAU_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO5532429.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14074..15618
                     /gene="gpmM"
                     /locus_tag="ACHKAU_003434"
                     /gene_synonym="pgmI"
     CDS             14074..15618
                     /gene="gpmM"
                     /locus_tag="ACHKAU_003434"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO5532430.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15652..16911
                     /gene="envC"
                     /locus_tag="ACHKAU_003435"
     CDS             15652..16911
                     /gene="envC"
                     /locus_tag="ACHKAU_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO5532431.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16915..17874
                     /locus_tag="ACHKAU_003436"
     CDS             16915..17874
                     /locus_tag="ACHKAU_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO5532432.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17861..18895)
                     /gene="waaH"
                     /locus_tag="ACHKAU_003437"
     CDS             complement(17861..18895)
                     /gene="waaH"
                     /locus_tag="ACHKAU_003437"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO5532433.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
BASE COUNT         4803 a         4881 c         4887 g         4403 t
ORIGIN      
        1 attcatacaa taaattatct gtaaattttt tcccagacat ggtttctttc atatttgaca
       61 tttattaata atcatcatta gaatccacga aaataatata ttcatacaaa cacagacatc
      121 ttttgaaacg aatgatacat ttttatgatg aatgcttaaa acaatattta atcgacacac
      181 atcactgtca ttaatgtaaa gcaacataga tcaccaataa tacaatggac aactctaccg
      241 gcattgatcg ctggtcggag gaaataagga tttttacatg aacttgaaga agattttttt
      301 cagtgctgtc actgtttcag ttctctgcgc attaacggga tgtgactaca ttgaagaagg
      361 caagccagaa agtagtttat tgaaacaaca agaagaacat aacaataaaa ttgatctact
      421 tgaaaaacaa caagcacagc taaagagtca actggaaact attcaaaaac aacaaactgg
      481 cataataagc agtacaaaga ctttaactca cgtaattaaa tccgttaaag atcaacaaaa
      541 cacttttatt tttaccgagt ttaacccagc aaaaacgaag tatttcatcc tgaacaatgg
      601 ttcggtggct ttagcgggtc gagtgttatc cattgacgcc acagagaatg gcagtgttat
      661 tcatatttca ctggtcaact tattaagtac acctatctca aatattggtt ttaatgcgac
      721 atggggtggt gaaaaacctg tcgatgccaa agagtttgcc cggtggcaac aattactttt
      781 caacacatca atgaaatcca cattgaaatt attaccaggt caatggcaag acattaattt
      841 gaccctgaag ggtgtatcgc ccaataacct gggatatctg aagttagcca tcaacatgga
      901 aaatattcag ttcgacaatc ttccttctgc tgaaaatcgg cagaaaagaa gcaaaaaaat
      961 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
     1021 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
     1081 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
     1141 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
     1201 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
     1261 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
     1321 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
     1381 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
     1441 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
     1501 atgggggact cttccaaagc ggaaggagca aacgccattg ccctgggaaa tgccactaaa
     1561 gctactgaga ttatgagtat tgctcttggc gacaccgcca atgcgtcaaa agcgtattca
     1621 atggcgctgg gagcaagtag cgtcgcatct gaagaaaacg caattgccct ggggcgtagc
     1681 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
     1741 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
     1801 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
     1861 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
     1921 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
     1981 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
     2041 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
     2101 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
     2161 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     2221 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     2281 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     2341 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     2401 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     2461 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     2521 gatgtcgaag ccaacaccgc caatatcgct actaatacca gcaacattgc cacgaatacg
     2581 gcaaatattg ccaccaatac caccaatatc accaacctga cggattccgt tggtgacctt
     2641 caggctgatg ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag
     2701 gataccacca gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat
     2761 gctgttaacg gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat
     2821 atcgccaata acacttccaa tattgccact aacaccacca acatctctaa cctgactgag
     2881 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg
     2941 gcagctcatg gcaccgagac caccagcaaa atcaccaacg ttaaagatgg cgacctgacg
     3001 actggcagca ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg
     3061 acgaatacca ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg
     3121 actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct
     3181 catggcaaca ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc
     3241 agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac
     3301 ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc
     3361 ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc
     3421 acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac
     3481 ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc
     3541 agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg
     3601 ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt
     3661 gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcttgac
     3721 gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga
     3781 aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc
     3841 agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg
     3901 ggtggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg
     3961 aacgccgagt acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa cgccttgctg
     4021 tgggatgaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc
     4081 agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac
     4141 ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag
     4201 ctcgctggta acaccgacgc aacctatatc caagaaaacg gtgcgggtat taactatgtg
     4261 cgtactaacg acgacggctt agcgttcaac gacgccagcg cacagggtgt tggcgctaca
     4321 gctataggtt ataactctgt cgccaaaggc gatagcagcg tagctattgg tcagggcagc
     4381 tacagcgacg ttgatacggg tatcgccctg ggtagcagct ctgtttccag ccgagtgatt
     4441 gccaaaggct cccgtgacac cagcataacg gaaaatggcg ttgttattgg ttacgacacc
     4501 acggatggcg aactgctcgg tgcattgtct atcggtgatg acggtaaata tcgtcaaatc
     4561 atcaacgtag ccgatggttc cgaagcccat gacgccgtta cggttcgtca attgcagaat
     4621 gcgattggtg cggtcgcaac cacgccgact aaatacttcc acgctaattc aacggaagaa
     4681 gattcactgg cagtgggaac tgactcgctg gcaatgggtg cgaaaaccat cgtgaatggc
     4741 gataaaggta ttggtatcgg ttatggtgcc tacgtggacg cgaatgcact taacggcatt
     4801 gccattggta gcaatgcgca agtcattcat gtcaacagta ttgcgatagg taatggttct
     4861 acgaccactc gtggcgctca aaccaattat accgcctaca acatggacgc accgcagaac
     4921 tctgtcggtg aattctcagt cggtagtgcg gatggtcaac gtcagatcac taacgtcgca
     4981 gcaggttcgg ctgataccga tgcggtcaac gtgggtcagt tgaaagtaac ggatgcgcag
     5041 gtttcccaga atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact
     5101 cgcgttacta atatcgaaaa cggtattggc gacattgtca cctccggtag caccaagtac
     5161 ttcaagacca acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt
     5221 ggttctggtt ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca
     5281 gaggaagaaa atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta
     5341 gctgccggtg ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca
     5401 ggcggcgtgc gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc
     5461 ggtggtggca acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac
     5521 gacgcggtga actacgcgca gttgaagcaa agcgtgcagg aaacgaagca atacaccgat
     5581 cagcggatgg ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt
     5641 atcgcttctg caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg
     5701 gcttctattg gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg
     5761 gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa
     5821 tactccgccg cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta
     5881 agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgctccggg
     5941 tcaacaatct aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc
     6001 ccccacaaga attggcccta ccaattcttc gcttatctga cctctggttc acaattcccc
     6061 aattaaaact cacatcaata ttgccaatac ataacattta gttaaccatt cattgtcatt
     6121 atccctacac aacacaattg gcagtgccac ttttacacaa cgtgtgacaa ggagatgagc
     6181 aacagactca ttacacgatg tgcgtggact ccaggagacc tgcaatgaat ctctggcaac
     6241 aaaactacga tcccgccggg aatatctggc tttccagtct gatagcatcg cttcccatcc
     6301 tgtttttctt ctttgcgctg attaagctca aactgaaagg atacgtcgcc gcctcatgga
     6361 cggtagcaat cgcccttgcc gtggctttgc tgttctataa aatgccggtc gctaacgcgc
     6421 tggcctcggt ggtttatggc ttcttctatg gtttgtggcc catcgcgtgg atcattattg
     6481 cagcagtgtt cgtctataag atctcggtga aaaccgggca gtttgacatc attcgctcgt
     6541 ctattctttc gataacccct gaccagcgtc tgcaaatgct gatcgtcggt ttctgtttcg
     6601 gcgctttcct tgaaggagcc gcaggctttg gcgcaccggt agcaattacc gccgcattgc
     6661 tggtcggcct gggttttaaa ccgctgtacg ccgccggact gtgcctgatt gttaacaccg
     6721 cgccagtggc atttggcgcg atgggcattc caatcctggt tgccggacaa gtaacaggca
     6781 tcgacagttt tgagattggc cagatggtgg ggcgtcagct accgtttatg accattatcg
     6841 tgctgttctg gatcatggcg attatggacg gctggcgcgg tatcaaagag acgtggcctg
     6901 cggtcgtggt tgcgggcggc tcgtttgcca tcgctcagta ccttagctcc aacttcattg
     6961 ggccggagct gccggacatt atctcttcgc tggtatcact gctctgcctg acgctgttcc
     7021 tcaaacgctg gcaaccagtt cgcgtattcc gcttcggtga cttgggggcg tcacaggttg
     7081 atatgacgct ggcccacacc ggttacaccg cgggtcaggt gttacgtgcc tggacaccgt
     7141 tcctgttcct gaccgctacc gtaacactgt ggagcatccc gccgtttaaa gccctgttcg
     7201 catcgggtgg tgcgctgtat gagtgggtga tcaacattcc ggtgccgtac ctcgataaac
     7261 tggttgcccg tatgccacca gtggtcagcg aggctacagc ctatgccgcc gtgtttaagt
     7321 ttgactggtt ctctgccacc ggcaccgcca ttctgtttgc tgcactgctc tcgattgtct
     7381 ggctgaagat gaaaccgtct gacgctatca gcaccttcgg cagcacgctg aaagaactgg
     7441 ctctacccat ctactccatc ggcatggtgc tggcgttcgc ctttatctcg aactattccg
     7501 gactgtcatc aacactggca ctggcactgg cgcacaccgg tcatgcattc accttcttct
     7561 cgccgttcct cggctggctg ggggtattcc tgaccgggtc ggatacctca tctaacgccc
     7621 tgttcgccgc gctgcaagcc accgcagcac aacaaattgg cgtctctgat ctgttgctgg
     7681 ttgccgccaa taccaccggt ggcgtcaccg gtaagatgat ctccccgcaa tctatcgcta
     7741 tcgcctgtgc ggcggtaggc ctggtgggca aagagtctga tttgttccgc tttactgtca
     7801 aacacagcct gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag gcttatgtct
     7861 taacgtggat gattccttaa tgattgtttt acccagacgc ctgtcagacg aggttgccga
     7921 tcgtgtgcgg gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga
     7981 gcgccaactg gcgatgcaac tcggcgtatc acgtaattca ctacgcgagg cgctagcaaa
     8041 actggtgagc gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta ttcgctggcg
     8101 tcatgacaca tggtcggagc aaaacatcgt ccagccgcta aaaacgctga tggccgatga
     8161 tccggattac agtttcgata ttctggaagc ccgctacgcc attgaagcca gcaccgcatg
     8221 gcatgcggca atgcgcgcca cacctggcga caaagaaaag attcagcttt gctttgaagc
     8281 aacgctaagt gaagacccgg atatcgcctc acaagcggac gttcgttttc atctggcgat
     8341 tgccgaagcc tcacataaca tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct
     8401 gcaatcctca gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca
     8461 actgaccgaa caacatcagg ctgtcattga cgccattttt gctggtgatg ccgacggggc
     8521 gcgtaaagca atgatggcgc accttagttt tgttcacacc accatgaaac gattcgatga
     8581 agatcaggct cgccacgcac ggattacccg cctgcccggt gagcataatg agcattcgag
     8641 ggagaaaaac gcatgattat ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt
     8701 ctgccgccgt tcctgttcca ctatatggat ggtggtgcat attctgaata cacgctgcgc
     8761 cgcaacgtgg aagatttgtc agaagtggca ctgcgccagc gtattctgaa aaacatgtcc
     8821 gacttaagcc tggaaacgac gctgtttaat gagaaattgt cgatgccggt agcactggca
     8881 ccggtgggtt tgtgtggcat gtatgcgcgt cgtggcgaag ttcaggcagc caaagcggcg
     8941 gacgcgcatg gcattccgtt tactctctcg acggtttccg tttgcccgat tgaagaagtc
     9001 gcgccagcca tcaagcgccc aatgtggttc cagctttatg tactgcgcga tcgcggcttt
     9061 atgcgtaacg cgctggagcg agcaaaagca gcgggttgtt cgacgctggt tttcaccgtg
     9121 gatatgccga caccgggcgc acgctaccgt gatgcgcatt caggtatgag cggcccaaat
     9181 gcggcaatgc gccgctactt gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc
     9241 ctgaacggtc gtccacatga tttaggtaat atctcagctt atctcggcaa accgaccgga
     9301 ctggaagatt acatcggctg gctggggaat aacttcgatc cgtccatctc atggaaagac
     9361 cttgagtgga tccgcgattt ctgggatggc ccgatggtga tcaaagggat cctcgatccg
     9421 gaagatgcgc gcgatgcagt acgttttggt gctgatggaa ttgtggtttc taaccacggt
     9481 ggccgccagc tggacggtgt actctcttcc gctcgtgcgt tgcccgctat tgcagatgcg
     9541 gtgaaaggtg atatcgccat tctggcggat agcggaattc gtaacgggct tgatgtcgtg
     9601 cgtatgattg cgctcggtgc cgacaccgta ctgctgggtc gtgctttcct gtatgcgctg
     9661 gcaacagcgg gccaggcggg tgtagctaac ctgctaaatc tgatcgaaaa agagatgaaa
     9721 gtggcgatga cgctgactgg cgcgaaatcg atcagtgaaa ttacgcaaga ttcgctggtg
     9781 caggggctgg gtaaagagtt gcctacggca ctggctccga tggcgaaagg gaatgcggca
     9841 tagtcgtttg cgccccctca ccctaaccct ctccctcagg gagaggggac cgttcggcgc
     9901 tgtatgtact ccctcactct gaaacgacac cgcactcttt ttttctccct cgcccctccg
     9961 gggtgagggg aaaaggccgc actgctcacc aattttctgc tatcctgccc ccgcactaag
    10021 ggggcagtat gctaaacatc gtactttacg aaccagaaat tccgccaaat actggcaata
    10081 tcatccgtct ttgcgctaat accggctttc gtctgcatat catcgaaccg atgggatttg
    10141 cctgggacga taagcgcctg cgccgcgcgg ggctggacta tcacgagttt accgccgtta
    10201 cgcgtcatca tgactttcgc gcgttcctcg aagcagaaaa tccccagcgc ctgtttgccc
    10261 tcactacgaa aggtacgcct gctcacagcg ccgtaaacta tcaggatggc gattatctga
    10321 tgttcggccc ggaaacacgc ggcctgccag cgagcattct tgatgccctg cccgctgaac
    10381 aaaaaattcg cattccaatg gtgccggaca gccgcagcat gaacctgtcc aatgcggtgt
    10441 cggtagtggt gtatgaagcc tggcggcagc tgggatatcc gggagcgttg ttgagagatt
    10501 agttactgta tgccggatag atgattacat cgcatccggc acgatcacag cacatcagat
    10561 cccatcccca tactcaaatg tatggttaat accgttgaaa tgctggtcca tatccattga
    10621 tggcttatcg ctgtctggtt taccgacaat acgagccgga acaccagcgg cggtagtatg
    10681 cggcggcacc ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc gaccaacttc
    10741 aatattgccg agaattttcg cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg
    10801 acggtcacca ccagatttac ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc
    10861 gttttcaatt accgccgttt caccaacgac gatgcccgtg gcgtggtcga gcatgatacc
    10921 gcgaccaatt tttgctgccg ggtgaatatc gacctggaac gtcacagaaa cctggttttg
    10981 cagaaagatt gccagtgcgc gacgcccctg attccacaac cagtgaccga tgcgataggc
    11041 ctgcaaggca tgaaaaccct tcaggtataa caacggggtt gagtatttat cgactgccgg
    11101 gtcgcgggta cgcaccgcct gaatatcaca ggccgcagag gcgatcattt ccgggtcagc
    11161 ggcgtaggct tcttcaacca cttcacggat agcaatagca ggcataattg gcgatgacag
    11221 cttgttcgcc agcatgtagc tcagggcact gccaaggttt tcgtgcttga gtagcgtcgc
    11281 gtggtaaaaa ctggccagca ttggctcaca gtccgccagc gttctggctt cggctttaat
    11341 attgttccag acaatttcca gttcttcaca cgacattgct tactccacac gatgagataa
    11401 tgaccggccc gttctgcgcg ggccgggtca tagcggtaac aaaggttccc tggggttagt
    11461 ggctgctgcg ctcgtccttg cgtgcacgac ctagtaaagt caatgctgcc tcgcgcgcgt
    11521 tttttccgca atataatact tgataaattt cctcggttat tggcatttca acgccgaagc
    11581 gatgcgccag ttcgcggact tctttcgtat tgcggtagcc ttccaccacc tgaccaatct
    11641 tctcctgcgc gctttgtaca tccatgccct gaccgagcat catgccaaaa cggcggttac
    11701 gcgactggtt gtcggtacag gtaagcacca gatcgcccag ccccgccatg cccataaagg
    11761 tggcaggatc ggcacccagc gccgcaccaa gacgcgacat ttcagccagc ccacgggtga
    11821 tcagcgccgt acgcgcattc gcaccaaaac cgataccgtc agacatcccc gcgccaatgg
    11881 caatgacgtt tttcaccgcg ccgccaagct gcacgccaat gaaatccggg ttgctgtaaa
    11941 cgcggaaact tttgccacag tgcagcaatt gttggagatc atcggcaaag gtctggtcgg
    12001 tagaggccaa cgaaatagcc gtcggtaaac ctgccgccag ttctttcgca aacgttgggc
    12061 cagagataac cgccagcgga atttgatcgc ctaaggcctc acgcgccacg tcctgtaaca
    12121 gacgtccggt ttccgcttcc agccctttgg tcgcccacac cagacgcgca tcaggacgca
    12181 tcagcggttt aatctggcgc agcacttcac caaagacatg gctgggtacg acgacgagaa
    12241 tattacggct ggctgccagc gcagtggcga gatcgctttc aagatggagc gtatcgggaa
    12301 aaggcacatc ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt
    12361 cagggtcatg gccccagagg acaacctcgt ggccatttct tgccagggta atggcaagag
    12421 cggtgccgta cgagccggca ccgatcacag tcattgaagc attacgttgg ttcatcaggc
    12481 atcctgatgt tcttcagtac cttcgccagc ctgctgctgc aaatagttca tgaacagcgc
    12541 atcgaagtta accggcgcaa ggttcagttg cgggaatgta ccgcgggata ccatgctggt
    12601 gatgcactca cgagcatacg ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc
    12661 catctgggtg ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa cttcacacag
    12721 gaacgcggtt tcttcgccca gggaggccgt tacggtaaca cgcagtacca cttcgtatac
    12781 gtcatctgcc agttgggaag atgccgtatc cagatcaagt ttaacttctg gttgccaatc
    12841 tttctggaaa acgtgcggcg cgttcggcgc ttcgaaagag atatccttgg tataaatacg
    12901 ttggatctgg aaagtcattt cagtgttgtt ttgttctgac atgtgtagaa aacccttaag
    12961 tgttgtcctt aaatactgcg caatgtaatg ccaacgttca cacgttattt cagcagggga
    13021 tccagtccac cacgtgcatc caatgcatac aagtcatcac agccgccaat gtgctgtgcg
    13081 tcaataaaaa tctggggaac cgtggtgcga ccgctgcgtt tgatcatctc ttcacgcttg
    13141 gcggcattgc catcgatcgg caactcctga aaactcacgc ccttgctgct cagcagtgct
    13201 tttgcacgat ggcaatacgg gcaggtttct ttggtataga tttcaacatt ggccataatt
    13261 tagctcctgt ttttttaccc tgaaatttca tgttgcaggg cggctgcaaa atggagtgtc
    13321 cccagaagct tataacaata agtcgctggg ggcctaattg cagctaacgg cctgcatcat
    13381 gaaagacgac aggtaaatta tttgccgcgc accagaggca agttttcgcc agcccagcca
    13441 gcaacgccct ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag cgcgtttgca
    13501 ggctcctggc actgcatgcc agaaccgtct accacgataa ccggtttgtc tttgtgcttc
    13561 tcaagctcac caacattgtt ggctttgatt tcactcggca acagattgat agaacctgcg
    13621 atatggcctt tacggaagtc atcacgctga cgtaaatcca caaccacagc gtcttctttg
    13681 ttgatcagac gcgtagcttc accacgagta atcaccttca ctttcgaggt caggctctta
    13741 aacgtagtca caagaaccgc caccagtaac gcgatccagg cgatactcag tatgggatga
    13801 cggccaacaa attgcataat ttcttgcatg gggggtaaca actcccgacg tagtgattaa
    13861 aaaaaccagg aaaggagtat acctgcgcgg tgcggcaaat acagccagcg cgttaactgg
    13921 aatgcaattt tgcggggcac gacgaaaaaa aagcgcaaat ctggcatcgg cttgccgcga
    13981 gcggtcgtat attttgatct tcagaggcta ttttatcgat tcagctgtag taaaattacg
    14041 caaattttga ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta
    14101 ctggtgattc tggatggcta tggctatcgt gaagaacagc aggataacgc catttttagt
    14161 gctaaaaccc cggtaatgga tgcactgtgg gccaatcgtc cgcataccct gatcgacgct
    14221 tccggtctgg aagtcggtct gcctgaccgt cagatgggta actccgaagt aggccacgtt
    14281 aacctgggtg ccggccgcat cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa
    14341 gatcgcgctt tctttgctaa tccagtgctg actggcgcgg tagataaagc gaaaaacgca
    14401 ggtaaagcag tacacattat gggtctgctc tccgcaggcg gcgtacacag ccacgaagat
    14461 cacatcatgg cgatggtaga actggcagct gaacgcggcg cagaaaaaat ctacctgcac
    14521 gcattccttg acggtcgcga caccccgccg cgcagtgctg aatcctcgct gaaaaaattt
    14581 gaagagaaat ttgctgcgct gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac
    14641 gcgatggacc gcgataaccg ttgggatcgc gttgaaaaag cctatgacct gctgactctg
    14701 gcgcagggtg agttccaggc cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc
    14761 gacgaaaacg acgagttcgt gaaagcgacc gttattcgtg cagaaggtca gccagatgcg
    14821 gcaatggaag acggcgatgc gctgattttc atgaacttcc gtgctgaccg cgcgcgtgaa
    14881 atcactcgtg cattcgtgaa cgctgatttc gatggcttcg cgcgtaagaa agtggttaac
    14941 gtcgatttcg tgatgctgac cgaatacgcc gctgacatca aaactgcggt tgcttaccca
    15001 cccgcttccc tggttaacac cttcggcgag tggatggcga aaaacgacaa aactcagttg
    15061 cgtatttccg aaaccgaaaa atatgcccac gttactttct tcttcaacgg tggcgtagaa
    15121 gagtcgttca aaggcgaaga tcgcattctg atcaactcac cgaaagtggc tacctacgat
    15181 ctgcaaccgg aaatgagctc cgcagagctg accgaaaaac tggttgcggc catcaagagc
    15241 ggcaaatacg acaccattat ctgtaactat ccgaacggcg acatggtagg tcacaccggg
    15301 gtgatggaag cggcggttaa agcggttgaa gcactggatc actgcgtgga agaagtcgcg
    15361 aaagcggttg agtccgtggg tggacaactg ctgatcaccg ctgaccacgg taacgctgaa
    15421 cagatgcgcg atccggcaac gggtcaggca cacacggcac acaccaacct gccagttccg
    15481 ctgatttatg ttggtgataa gaacgtgaaa gcggttgaag gcggcaaact ttctgacatc
    15541 gcgccgacca tgttgtcgct gatgggtatg gaaatcccgc aagagatgac tggtaagccg
    15601 ctgttcatcg tggaataatc cctccccatg aggggaaagg cgattaatac catgacacgg
    15661 gccgtgaaac cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt tcttagcgct
    15721 ggcgtattgt tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact caaatctatt
    15781 caggccgata tcgccgcaaa agagcgcgcg gtacgccaaa agcaacaaca acgcgcaagc
    15841 ctgctcgcac aattgaaaaa gcaggaagaa gcgatctctg aagccacccg taagctgcgc
    15901 gaaacgcaaa acacgctcaa tcaactcaat aaacagattg atgagatgaa cgcgtcgatt
    15961 gccaaactgg agcagcaaaa agccgcccag gagcgcagcc tcgccgcgca actggatgcc
    16021 gcattccgtc agggcgagca taccggtatt cagctaattc tcagcggtga agaaagccag
    16081 cgtggacagc gtttacaggc ttatttcggc tatctcaacc aggcgcgaca agaaaccatt
    16141 gcccagttga agcaaacgcg tgaagaagtc gccatgcagc gtgctgaact ggaagagaaa
    16201 caaagcgagc aacaaacgct gttatatgaa cagcgcgccc aacaggcgaa actgactcag
    16261 gctctgaacg agcgtaaaaa gacgctggcg gggctggaat cttccatcca gcaaggtcag
    16321 cagcagctaa gtgaactgcg tgccaacgaa tcccgtctgc gtaacagcat tgcccgtgcg
    16381 gaagccgcgg cgaaagcgcg tgctgaacga gaagcgcgtg aggcccaggc ggttcgcgac
    16441 cgccagaaag aagcgacgcg caaaggcacc acctataaac cgaccgaaag cgaaaaatcg
    16501 ctgatgtccc gaactggtgg cctgggtgcg ccgcgtggtc aggcattctg gccggttcgc
    16561 gggccgacgc tgcatcgcta tggtgaacag ctacagggcg aactacgctg gaaaggaatg
    16621 gttatcggtg cctctgaagg tactgaagtt aaagcgattg ccgacggtcg ggtgattctg
    16681 gctgactggc tgcaaggtta cggtctggtg gtggtggttg agcacggtaa aggcgacatg
    16741 agtctttacg gctataatca gagcgcactg gtgagcgttg gttcgcaggt tcgcgcgggc
    16801 cagccaattg cactggtggg cagcagtggc ggtcagggtc ggccttcact ctatttcgaa
    16861 attcgccgcc agggtcaggc ggtcaatcca cagccgtggt tgggaagata agttttgttt
    16921 ccatttcgtc gtaacgttct tgcatttgcc gctctgttgg cgctctcctc ccccgtactt
    16981 gctggcaaac ttgccatcgt cattgatgat tttgggtatc gcccgcacaa cgaaaaccag
    17041 gtgctggcga tgccctccgc tatctccgtc gctgtattac ccgattcacc gcacgccaga
    17101 gagatggcga ccaaagcgca taacagcggg cacgaagtgt tgattcatct cccgatggca
    17161 ccactgagta aacagccgct ggagaaaaat acgctgcgcc cggagatgag cagcgacgaa
    17221 attgagcgca ttattcgtag tgcggtcaat aacgtgccct atgccgtggg gatcaacaac
    17281 cacatgggta gcaagatgac ctctaacctg tttggtatgc agaaagtgat gcgggcactg
    17341 gagcgttaca atctttactt cctcgacagc gtaaccatcg gtaataccca ggcgatgcgc
    17401 gccgcgcaag gtactggcgt gaaggtgatc aaacggaagg tgttcctcga cgattcgcag
    17461 aacgaagcgg acatccgtgt gcaatttaat cgcgcaattg atctggcgcg tcgcaacggt
    17521 tcaaccattg ccattgggca tcctcaccct tcaacggtac gcgtgctaca acagatggtt
    17581 tataacctgc cgccagacat tacgctggtg aaagccagca gcttgctgaa tgaaccgcag
    17641 gttgatactt ctacaccgcc gaaaaacgct gtgcctgatg caccgcgtaa tccgttccgt
    17701 ggcgtgaagc tgtgcaaacc gaagaaaccg atagaacctg tttatgctaa tcgcttcttt
    17761 gaagtattaa gcgaaagcat cagccagagc acgctgatcg tttacttcca gcatcagtgg
    17821 caaggctggg gcaaacagcc cgaagcggcg aagtttaacg ctagcgcaaa ttaaaggcgc
    17881 tacgcgtaat gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag cggaaagacc
    17941 acagtaacgc ctggtaaccg accttcacgc tgcgcacatt ggtaatcagg cgcttataca
    18001 taccggaagt aaatatctcg gcaatcatcc gttggcgggt aagaatatcc ggctctttgc
    18061 gcaccgcatg gcaaacacgc aatgcttcgt aagttatttg ctgatgaaat tccggataaa
    18121 tcgtaatttt gtcggcataa tttcgattta atttctccag caggcgggta atcttaatat
    18181 agtgacgttg ataattaagg tttttattcc cttgtctatg caaccgactc actgacgtat
    18241 tatgcagata atatttatat aatgattgct cggtatatcg cgcacgcagc gcgttaaaca
    18301 tgaattctgt tgtccagaca atatcctgat gatgtaatcc ggcaataaat ttaatgttat
    18361 ttttaacaat aacatcacgg cgataaaccc ccatccagac aacgtgagtc caacggcgcg
    18421 aagaaagccc catccgcagc cagtccgggc cggttaatac gccggttgag cgaaggcgat
    18481 cgctggggat ggattgccag gtttctcccg tttcacgaaa acaccagtca gcgttgcact
    18541 gcgccacgtc gaggtcatcc tctaacgcca tggtcatcag cgtttcgtac atggtgggat
    18601 agacctcatc gtcagcatcg acaaaagcga catatttgcc cgtcgccacc tcgatcccac
    18661 gattacgcgc taccgatgcg ccagcattcg cctgatgcaa caaacgaacg tgcggatagt
    18721 tttctgcgta atgctttgct atttcaacag aattatccgt tgaaccatcg ttaataataa
    18781 tgatttccag agcagtccag gtttgcgtaa ttaaagattc catacaagtg cggaaatcat
    18841 cgcccgcatt atataacgga ataataacac taagtttatt ggtgctgttc atcataaatt
    18901 accgacgacc tctgaagtta tcctgacgtt ttacaggctt tgtattaaga aactattaag
    18961 cctgtgagat atta
//
LOCUS       DAZFJF010000090        18912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFJF010000090 DAZFJF010000000
VERSION     DAZFJF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.090"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..943
                     /locus_tag="ACHKAU_003438"
     CDS             <1..943
                     /locus_tag="ACHKAU_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO5532434.1"
                     /translation="LFLFSLPLIIFYQPWVNALPPMPRHANPEQLEKTVRYLTQTVHP
                     RSADNIDNLNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGA
                     HYDSASSYENDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFF
                     RSDEMGSAVHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVV
                     GRMQDINAVRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTA
                     FYRNKQYHLPGDTADRLNYQKMAQVVDGVINLLYNSK"
     gene            1046..1549
                     /locus_tag="ACHKAU_003439"
     CDS             1046..1549
                     /locus_tag="ACHKAU_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO5532435.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1616..3073)
                     /gene="nuoN"
                     /locus_tag="ACHKAU_003440"
     CDS             complement(1616..3073)
                     /gene="nuoN"
                     /locus_tag="ACHKAU_003440"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO5532436.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3080..4609)
                     /gene="nuoM"
                     /locus_tag="ACHKAU_003441"
     CDS             complement(3080..4609)
                     /gene="nuoM"
                     /locus_tag="ACHKAU_003441"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO5532437.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4773..6614)
                     /gene="nuoL"
                     /locus_tag="ACHKAU_003442"
     CDS             complement(4773..6614)
                     /gene="nuoL"
                     /locus_tag="ACHKAU_003442"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO5532438.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6611..6913)
                     /gene="nuoK"
                     /locus_tag="ACHKAU_003443"
     CDS             complement(6611..6913)
                     /gene="nuoK"
                     /locus_tag="ACHKAU_003443"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO5532439.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6910..7464)
                     /gene="nuoJ"
                     /locus_tag="ACHKAU_003444"
     CDS             complement(6910..7464)
                     /gene="nuoJ"
                     /locus_tag="ACHKAU_003444"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO5532440.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7476..8018)
                     /gene="nuoI"
                     /locus_tag="ACHKAU_003445"
     CDS             complement(7476..8018)
                     /gene="nuoI"
                     /locus_tag="ACHKAU_003445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO5532441.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8033..9010)
                     /gene="nuoH"
                     /locus_tag="ACHKAU_003446"
     CDS             complement(8033..9010)
                     /gene="nuoH"
                     /locus_tag="ACHKAU_003446"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO5532442.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9007..11733)
                     /gene="nuoG"
                     /locus_tag="ACHKAU_003447"
     CDS             complement(9007..11733)
                     /gene="nuoG"
                     /locus_tag="ACHKAU_003447"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO5532443.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGLVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11786..13123)
                     /gene="nuoF"
                     /locus_tag="ACHKAU_003448"
     CDS             complement(11786..13123)
                     /gene="nuoF"
                     /locus_tag="ACHKAU_003448"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO5532444.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13120..13620)
                     /gene="nuoE"
                     /locus_tag="ACHKAU_003449"
     CDS             complement(13120..13620)
                     /gene="nuoE"
                     /locus_tag="ACHKAU_003449"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO5532445.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13623..15425)
                     /gene="nuoC"
                     /locus_tag="ACHKAU_003450"
     CDS             complement(13623..15425)
                     /gene="nuoC"
                     /locus_tag="ACHKAU_003450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO5532446.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15519..16181)
                     /gene="nuoB"
                     /locus_tag="ACHKAU_003451"
     CDS             complement(15519..16181)
                     /gene="nuoB"
                     /locus_tag="ACHKAU_003451"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO5532447.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16197..16640)
                     /gene="nuoA"
                     /locus_tag="ACHKAU_003452"
     CDS             complement(16197..16640)
                     /gene="nuoA"
                     /locus_tag="ACHKAU_003452"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO5532448.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17271..18209)
                     /gene="lrhA"
                     /locus_tag="ACHKAU_003453"
     CDS             complement(17271..18209)
                     /gene="lrhA"
                     /locus_tag="ACHKAU_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO5532449.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4752 a         5228 c         4836 g         4096 t
ORIGIN      
        1 attatttctg ttttcactcc ctcttattat tttttaccaa ccctgggtca atgcattacc
       61 gccaatgccc cgacacgcaa atcctgagca attagaaaaa acagtgcgtt atcttacaca
      121 aactgtgcat ccacgtagtg ccgacaatat tgataatctg aataggtccg cagagtatat
      181 aaaagaggtc ttcgtcagta gcggtgccag aattacctcg caggacgtcc ccattacggg
      241 tggcccctac aaaaacattg ttgctgatta tggtcctgcc gatggaccgc tgattattat
      301 tggtgcgcac tatgactctg ccagcagtta tgaaaacgat gaattgacct atacacctgg
      361 cgcggatgat aacgccagcg gcgtggcagg attactcgaa ctggcacgtt tgttacatca
      421 gcaagtaccg aaaacaggcg tgcagctggt cgcctatgcg tcggaagaac cgcccttctt
      481 tcgtagcgat gaaatgggga gcgcggtaca tgcagcttcg cttgagcgtc cagtaaaatt
      541 aatgatagca ctggagatga ttggctatta cgactctgcg cctggtagcc aggattaccc
      601 ttacccggca atgtcctggc tttatcccga tcggggagac tttattgccg tggtcggcag
      661 aatgcaggat atcaacgccg ttcgtcaggt aaaagcgaca ttgttatcat cccggtattt
      721 atctgtttat tctatgaatg ccccagggtt tattcccggt attgatttct ctgatcacct
      781 taattattgg caacacgata ttcccgccgt aatgattact gacaccgctt tttatcgtaa
      841 taaacaatac cacttgcccg gtgataccgc agacagattg aattatcaga aaatggctca
      901 ggtggtggat ggtgttataa atttgttata caacagtaaa taataaaaat ttctcgaggc
      961 gggtccagaa aaagcgacat gcacatttta ttttccctcg tctgttttaa gatgcgaaac
     1021 atgaaattca atggaggaaa aataaatggg aatgattggt tattttgcag aaattgactc
     1081 agaaaagatt aaccaactgc ttgagtctac tgaaaaacct ttgatggata atatacatga
     1141 cacgctttca ggattacgcc gactggatat tgataaacgc tgggattttt tacactttgg
     1201 gctgacaggc acctctgctt ttgatcccgc gaagaatgat ccgctgagtc gtgccgtgct
     1261 tggtgaacac agcctcgaag atggcataga tggcttcctg ggattgacct ggaatcagga
     1321 gttagccgcg acaatcgacc gactggaatc actggatcga agtgagctgc gtaaacagtt
     1381 ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt cagaagagtt
     1441 agaggggcaa ctctttgctt cgatcatgct tgatatggaa aagctgatta gcgcgtatcg
     1501 acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat ggtgtcaggg
     1561 caccaaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaacta acagattaca
     1621 tcagcggcat tgctaaccgc acaatgctaa tcagcggttg tggccataca cccagcacca
     1681 gtaccaacag tgcagaaatc agcaccacga taccgcccgc gctgtactgc cagtttgatg
     1741 gtgcatcgcg acccggttgt tccggggcgt gaagatacag gctcaccgcc acgcgcaggt
     1801 agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac cacaagtgtg
     1861 cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc atcgggatac
     1921 cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga tgccagaaca
     1981 gaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg gacatcaggc
     2041 tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa acccctaccg
     2101 cttccatcga catctcacca gtttgcagcg caatcagcgc caccagcaga tagccgaggt
     2161 gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcagc gccatcaggt
     2221 taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc gcttcgctgt
     2281 cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg
     2341 ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca
     2401 ggtggaacgg caccagcgag agtttgaagc cgaggccaac aatcatcagt ccgaaacctg
     2461 ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc gcgacaaacg
     2521 acaggtcgcc agactgtgca tacaccagcg ccataccaaa cagcaggaaa gaagacgctg
     2581 cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg cggaaagcgt
     2641 aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac aaagacgcca
     2701 gatggttggc attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga
     2761 actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga
     2821 ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg
     2881 gcgtaacgtc catagcgccc gcctggccga caaaccagag cgaaaccagc gccgcgttaa
     2941 gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca
     3001 tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg
     3061 gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct
     3121 ggatattgcc aatcgcggag cgcgaggtat ccagaatcgg ctgcggatag aagcccagca
     3181 gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca
     3241 gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc aacatcgcca
     3301 gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatc accgggacaa
     3361 cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc
     3421 caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca
     3481 gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccacaaagaa
     3541 taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg ccctggtagg
     3601 ccaactggct gccggtgtag atagcaatca gcacgaagcc catgtgggaa accgaggtgt
     3661 aggcgatcag acgtttgata tcggtctggg cgaaggccat ccacgcaccg tagaagatgc
     3721 cgataacacc cagccacata gcaattggcg cgaactctgc cgacgcgttc gggaacagcg
     3781 gcagggagaa acgcagcaaa ccgtaagcgg cagttttcag caagatcccc gcgaggtcaa
     3841 cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc agcggaacca
     3901 ccggcatttt gactgcgaag gcgatgaaga agcccagcat taacaggtat tccacaccac
     3961 tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt
     4021 agtgaacaaa aaccagcgcc aggatggcaa tcaacatcac caggccactc gcctgggtgt
     4081 aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc
     4141 acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga
     4201 acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt
     4261 ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc agcacaccga
     4321 gcagaccggt cagcacgacc atcagcagcg acagcccgtc aatggcgaga tgaatagaga
     4381 taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg
     4441 attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc
     4501 ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg gtctgccagc
     4561 acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta
     4621 ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct
     4681 cttcggactg ggggtagtgt cggatgcacc gcgaaggcgg cttatccgac ctacgttctt
     4741 atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc
     4801 accacgaccg caccgatgct catggatgcc acataccagc gcagatagcc gttctcactt
     4861 aacagcagac ctttacctgc aaagcgggaa aggacagccg ggatgttcat cattgagttc
     4921 agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa cactttgtca
     4981 tacagccagt caaatcccca ggcgttgtac caccaggtgc ccagcagacg gcccggcgca
     5041 ctgttggcga tggaggtcac cagagtacgt ttacccagcc acagccaggc tgccagcaga
     5101 atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc
     5161 agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag
     5221 gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga
     5281 gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag
     5341 gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt cgccatcgca
     5401 cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggtag tgccgacagt
     5461 gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc
     5521 atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac
     5581 agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc
     5641 acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt
     5701 ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca
     5761 atacccacca gatgcagaac ttccggcgtc accaggaaca ggccgtgggt acgggcgatc
     5821 aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac aggcgtcggg
     5881 cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca
     5941 ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc aaagtgcgct
     6001 ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta aagaatgaac
     6061 agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt cattgccgct
     6121 gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg
     6181 ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac taccatgctg
     6241 gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac
     6301 caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag catggtcagc
     6361 gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga catccacgtc
     6421 cacagcggct ggctgtatga ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg
     6481 gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac
     6541 cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag
     6601 gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac
     6661 ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg
     6721 cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg
     6781 ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac
     6841 ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt
     6901 gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct
     6961 cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag
     7021 cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac
     7081 tgctttagca ctgattggcg taccgtcgat accctgatca ttaacaccaa ggatggcgta
     7141 aacaatcacc accagcatga tggccgacaa aattgccgga ccaatccaca cctgcggttt
     7201 cagccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca tcaccacgaa
     7261 cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta
     7321 agcgcccagt gagaagaaca ccccggaaat cgccagcagc gaaataatca ggtacagcag
     7381 tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc
     7441 acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg
     7501 ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc
     7561 atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttt
     7621 tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc
     7681 gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga gaagttgatg
     7741 cggaaaaatt ccgggtacca gcgaccgtct ttggtttctg ctttttgcag cgagatacag
     7801 ccgaccgggc aggctaccgc gcagaggtta caggctacgc aacgctcttc gccgtccggg
     7861 tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg
     7921 tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat actacgaacc
     7981 tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg
     8041 cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga
     8101 ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac gcacgaatca
     8161 aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca
     8221 acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc
     8281 cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca atgtggtaac
     8341 catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cataccgcca
     8401 cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gataacgttc cacacatgcg
     8461 cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc acgcccatca
     8521 gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccca
     8581 gcaacgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa accgccagac
     8641 ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccggac
     8701 tgactggcac aatcgcaaag gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg
     8761 taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac attttgatca
     8821 tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtccg taacggttct
     8881 ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccgcag gtgacaacca
     8941 ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata
     9001 tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc
     9061 aatgccggac atacccatcg gcaaacccac cagccctgcc gtcagccctt cagcgatttc
     9121 aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgagcgc ctgcgttcac
     9181 gcccaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacggctctg
     9241 gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtaat acggcgcgat
     9301 acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc
     9361 attttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac
     9421 ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc aggcaaacgg
     9481 cacttgcgaa cggtgcgcag tcggctggtt gttaccttcc atcgagaagg tgaacatggt
     9541 gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg
     9601 accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc
     9661 cggcgcagca tctttgatac ctgccagttc cgggattttc gccacaacag cgtcaatcac
     9721 atggtcgagc tgcgtccagt ccacttcacg gctcagcagg gtgctgtgca gcgagtgtaa
     9781 ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagcaggat cgtaaacctg
     9841 gaagaaacgt tgggcgcggc cttcgttgtt gatcaccgta ccgtcgcttt cagcaaagct
     9901 ggcagcagaa agtaccagat gggcgttttc cataatcgct gtgcgttgat gatcaaccac
     9961 catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcggaag cgtgacgatg
    10021 cagatcgttt tccagcacca ccaccgcgtc ggcgcgtccg gtttccagtt cggtcaacgc
    10081 ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat
    10141 catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc
    10201 ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc
    10261 tgccagtgcc tgcacgatga cgtcgatttt gctttgcagc tcaggttcga taccgtcaac
    10321 cgctggcgca gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc
    10381 aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgggtgtcat caacgttggt
    10441 aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa tctgccagtc
    10501 agccactttc tgtgctgccg ccatttcgcg cgctttacct ttcacagcct gacgcactgc
    10561 cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta
    10621 agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc
    10681 cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac
    10741 cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac
    10801 tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt
    10861 aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca ggttgacgta
    10921 accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg
    10981 gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac agccgatgga
    11041 acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga
    11101 gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc
    11161 gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg caccgtaaac
    11221 gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt agcaggcgat
    11281 gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt gggtacgttt
    11341 ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct gaagatggca
    11401 gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta accactcgac
    11461 cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga
    11521 agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg
    11581 cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc agcaaaagta
    11641 aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc
    11701 gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt tctcagtcag
    11761 agactgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc
    11821 cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga
    11881 aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt
    11941 tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc ggctgacctt
    12001 caccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg
    12061 tacaccagcc gcaggattca cgggcgaaaa actcttccag gttacgcacc agcgacacca
    12121 tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctt cctgctttac
    12181 cgatactttc gaattccatc ggcaggtcaa gatgcgcttc ggtcaggaag tcggtgcccg
    12241 cgccgcctgg ctgccaggct ttgaatttca gaccgtcacg cataccaccg gcgtaatctt
    12301 cgaggatctc gcgcgcggta gtaccaaacg gcagttccca caggcccgga tttttcaccc
    12361 gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc
    12421 actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacac
    12481 aggtcggttt accccatgcg ccggaggtcg ccgggaatgg aggcttcgag cgtgggttag
    12541 cacgacgtcc ttccagggag ttgattaacg ctgtttcttc cccgcagatg tagcgccctg
    12601 ccccggtatg gacgaacagt tcgaagtcaa aacctgttcc cataatattt ttgccaagca
    12661 gacctgcttc ggtggcttcg gcaatggcac ggcgtagatt cactgccgct tcgatatatt
    12721 cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag atgagcatac
    12781 cttccaccag caggtgcggc agttgctcca tcaacaggcg gtctttatag gtgcccggct
    12841 ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca
    12901 ggctccactt caggccagtc gaaaagcccg cgccgccgcg ccctttcaga ccagcgtctt
    12961 ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc gccccttcgt
    13021 aaccattttt gctgcggtat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc
    13081 aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag
    13141 ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca tcatatttgg
    13201 ccctttatca cagttcccca ggcagcaggt tggcagcagc gtaaagcgac cgtcaaacgt
    13261 cgtttgccct ggtttgatgt tcagtttttt ctccagcgcc gcctgaatac cctggtaacc
    13321 gttgatgtga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa ccggttggcg
    13381 gaagatctgg ctatagaacg ttgccacgcc ttcgacatcg ctcgccggaa tacccagcac
    13441 atcggcgatc gcgtggatcg cgccgtccgg cacccagcca cgctgcttct gaacgatttt
    13501 cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc
    13561 ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat
    13621 aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca
    13681 gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg
    13741 cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta actgttgatc
    13801 cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac
    13861 acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc
    13921 ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc
    13981 tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag
    14041 tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga
    14101 cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc
    14161 cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg
    14221 gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac
    14281 tcacgcagca ggcgatccca gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac
    14341 cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat tttctgacga
    14401 tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg
    14461 tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat gacgttaacg
    14521 cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta
    14581 acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg ccaggactgg
    14641 cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca
    14701 atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga cgggtggttc
    14761 ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat cccccactct
    14821 tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac
    14881 ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc tttccaggtt
    14941 tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc aaacagatcc
    15001 caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc
    15061 aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc
    15121 agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt
    15181 tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa
    15241 tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc
    15301 gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat taccggatca
    15361 tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc
    15421 accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa tagatttagg
    15481 aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc
    15541 aggttggtta cggcaatacg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg
    15601 cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat
    15661 tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata
    15721 tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata
    15781 ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag catctggtca
    15841 tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg
    15901 tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc
    15961 gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa
    16021 attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg
    16081 ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg
    16141 ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag
    16201 cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg
    16261 ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa
    16321 ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat
    16381 gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac
    16441 ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg
    16501 tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa
    16561 cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca
    16621 gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc
    16681 acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaatcactg
    16741 tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat aaccttttgt
    16801 caactttaac aaaagtttct tcacattagt ttacataata tcaacaccat tagcatttaa
    16861 tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt gaatcgtgtc
    16921 cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg tcgattcctc
    16981 cccccaaaag agagtatttt cttgatctgt gacacgcttt tgtcattcca taacaaaaac
    17041 gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt ttcaggcgat
    17101 tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa atgaacagtt ttgtacgttc
    17161 tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa aacaccccca
    17221 gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcgata
    17281 tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac
    17341 ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt attactggac
    17401 gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc
    17461 agcacacgca ggtccgggct catcatctca accggccttg ccgtcacgcc aagaccggct
    17521 ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata
    17581 tctgctttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc atccagcaat
    17641 acaagaggga tcggctcgcc tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt
    17701 ggcgaggttc gcaggttcaa tgccttaaac gctgaaggac gatgggtggt taccatcaaa
    17761 tccacttcct gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc
    17821 agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg
    17881 gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat
    17941 gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca
    18001 tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt
    18061 tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg
    18121 gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg
    18181 ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc tcatactgag
    18241 ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag
    18301 tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc
    18361 acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat
    18421 ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata aatgtaaatg
    18481 tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg tacaaacagc
    18541 aatggtgagg cggcatcaag agcagggatc cgtcatttat gcgagcattt taccccaaaa
    18601 accttttatt tataagggtc attgcgaatt atctgatgca aagttatgtt atgttaggca
    18661 aagtaatctt ctattattaa taagcacatc aaaacctttt tgaatattaa ataataatta
    18721 attagcatca tcctcattca ttaattccgc ttaacaatag tttcacacct tgcccccatt
    18781 acgacacctc ggaaatcatc acgctgttaa actctccgta atgcggaaat atcatcatcg
    18841 cgtttcatta gtgaattctt aaatgaggca ttttcacaca attatcttac agataaaaaa
    18901 accagactta ca
//
LOCUS       DAZFJF010000091        18568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFJF010000091 DAZFJF010000000
VERSION     DAZFJF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.091"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            154..1374
                     /locus_tag="ACHKAU_003454"
     CDS             154..1374
                     /locus_tag="ACHKAU_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5532450.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFPCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1347..1976
                     /locus_tag="ACHKAU_003455"
     CDS             1347..1976
                     /locus_tag="ACHKAU_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5532451.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1977..3137)
                     /gene="ybdL"
                     /locus_tag="ACHKAU_003456"
     CDS             complement(1977..3137)
                     /gene="ybdL"
                     /locus_tag="ACHKAU_003456"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO5532452.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3246..4334
                     /gene="hcxA"
                     /locus_tag="ACHKAU_003457"
     CDS             3246..4334
                     /gene="hcxA"
                     /locus_tag="ACHKAU_003457"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO5532453.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4344..4541)
                     /locus_tag="ACHKAU_003458"
     CDS             complement(4344..4541)
                     /locus_tag="ACHKAU_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5532454.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4723..6828)
                     /gene="cstA"
                     /locus_tag="ACHKAU_003459"
     CDS             complement(4723..6828)
                     /gene="cstA"
                     /locus_tag="ACHKAU_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO5532455.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7009..7422)
                     /gene="entH"
                     /locus_tag="ACHKAU_003460"
     CDS             complement(7009..7422)
                     /gene="entH"
                     /locus_tag="ACHKAU_003460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO5532456.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7425..8171)
                     /gene="entA"
                     /locus_tag="ACHKAU_003461"
     CDS             complement(7425..8171)
                     /gene="entA"
                     /locus_tag="ACHKAU_003461"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO5532457.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8171..9028)
                     /gene="entB"
                     /locus_tag="ACHKAU_003462"
     CDS             complement(8171..9028)
                     /gene="entB"
                     /locus_tag="ACHKAU_003462"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO5532458.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9042..10652)
                     /gene="entE"
                     /locus_tag="ACHKAU_003463"
     CDS             complement(9042..10652)
                     /gene="entE"
                     /locus_tag="ACHKAU_003463"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO5532459.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10662..11837)
                     /gene="entC"
                     /locus_tag="ACHKAU_003464"
     CDS             complement(10662..11837)
                     /gene="entC"
                     /locus_tag="ACHKAU_003464"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO5532460.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12206..13162
                     /gene="fepB"
                     /locus_tag="ACHKAU_003465"
     CDS             12206..13162
                     /gene="fepB"
                     /locus_tag="ACHKAU_003465"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5532461.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13166..14416)
                     /gene="entS"
                     /locus_tag="ACHKAU_003466"
     CDS             complement(13166..14416)
                     /gene="entS"
                     /locus_tag="ACHKAU_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO5532462.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14527..15531
                     /gene="fepD"
                     /locus_tag="ACHKAU_003467"
     CDS             14527..15531
                     /gene="fepD"
                     /locus_tag="ACHKAU_003467"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO5532463.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15528..16520
                     /gene="fepG"
                     /locus_tag="ACHKAU_003468"
     CDS             15528..16520
                     /gene="fepG"
                     /locus_tag="ACHKAU_003468"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO5532464.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16517..17332
                     /gene="fepC"
                     /locus_tag="ACHKAU_003469"
     CDS             16517..17332
                     /gene="fepC"
                     /locus_tag="ACHKAU_003469"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5532465.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17329..18462)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKAU_003470"
     CDS             complement(17329..18462)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKAU_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO5532466.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4372 a         5253 c         4790 g         4153 t
ORIGIN      
        1 tttttgatat ataactacag acagctacta taactttcat ctatttattc acagcgcgaa
       61 tgaaaaataa aacactctgc tttgcagtgt ttactttata attaacagac ttatctcact
      121 gatcaccctg taacgttcag agagcgtctt cagatgtcta tttataaaat tcctcttccg
      181 ctcaatattc tggaagctgc acgagagcgt attacctgga cactcaacac cctaccccgc
      241 gtttgtgtct cattttctgg cggcaaagac tctggtttaa tgctgcatct gacagccgaa
      301 cttgcccgac aaatgggcaa aaaaatctgc gttttgttta tcgactggga ggcgcaattc
      361 ccttgcacca ttaactatgt tcagtccctg cgcgagttgt acaccgatgt catcgaagag
      421 ttttactggg ttgcgctccc gcttacgacg caaaattccc tttcacaata ccaacccgaa
      481 tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc ccccgcaaga tgcgataacc
      541 gatcctgact ttttctgctt ttaccagcca ggcatgacct tcgaacaatt tgtacgtgag
      601 tttgccgaat ggttttcaca aaaacgtccg gcggcgatga tgatcggcat ccgtgcggat
      661 gagtcctaca accgttttgt cgccatcgcc agtttaaata aacaacgttt tgccgacgat
      721 aaaccatgga ctacggccgc accaggcggt catagctggt acatttaccc catttacgac
      781 tggaaagtgg ctgatatctg gacctggtat gcaaatcatc aaaatctctg caacccactg
      841 tataacctaa tgtatcaggc aggcgttcct ctgcgtcata tgcgaatttg cgaacctttt
      901 ggcccggagc aacgacaagg attatggctc tatcacgtta tcgaaccgga tcgctgggct
      961 gctatgtgcg cacgagtcag cggggtaaaa agtggcggca tttacgccgg acatgataat
     1021 catttctatg ggcatcggaa aatcctcaag ccagaacatt tagactggca agaatatgca
     1081 ttattgctgc tcaatagcat gccggaaaaa acagctgagc attaccgcaa taaaatcgcc
     1141 atttatttgc actggtatca gaaaaaaggc atcgaggttc cacaaaccca gcaaggggac
     1201 attggcgcga aagatatccc ctcctggcgg cggatatgca aagttttact caataacgat
     1261 tactggtgtc gggcattatc atttagtcct acgaaagcga agaactatca gcgttataac
     1321 gaacggataa aaggaaaacg tcaggaatgg gggatactat gcaacaacga ttaacgcagg
     1381 atttaacgca atttctcgcc agcttgccgg aagatgatcg catcaaagcg attaatgaaa
     1441 tccgcatggc gatccatcag gttagcccct ttcgtgaaga gccagtggat tgcgttctgt
     1501 gggttaaaaa cagccaactt atgcctaatg attacaaccc aaacaacgtg gcaccacccg
     1561 aaaaaaaact gctgaaaaaa tccattgaaa tagatggttt tacccaaccc attgtggtca
     1621 cgcatacgga taaaaatgcc ctggaaatcg tggatggttt tcaccgccat gaaattggta
     1681 aaggctcatc atcattaaaa ttacgcttaa aaggttattt gccggtgacc tgtctggaag
     1741 gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa tcgcgctcgt ggacgccatc
     1801 aaatcaccgc catgtcggaa atcgtccgtg aacttagcca gttgggatgg gacgataata
     1861 aaattggcaa agagctagga atggacagcg acgaagtgct gcgcctgaag caaattaacg
     1921 gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc ctggacggtt aaataactaa
     1981 agctggcgca ggcgttcagc tgctgccagc aacgtcgatt ccttcttggc aaaacagaga
     2041 cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg acagcggaat cgccgctacg
     2101 ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat ccagggtaga aacctcgctg
     2161 taatccacca gcaaaaagta tgtaccttca cacggtaaaa tctccagccg gctttcattt
     2221 aaagcattca ccagaatatc gcgcttctgg cgataaaagt ccggtaacgc aagataatgc
     2281 tcaggttctg cacgtagcat atcagcaagc gccagctgtg ccggggtatt caccgaaaag
     2341 gtcagatact gatgtacctt gcgaatttcg gcgctgatgg gcgctggcgc aacacaataa
     2401 cccactttcc agccggtcat atgataggtc ttgccaaatg aagaaaccgc cactgcccgc
     2461 tcacgcagct gcggatgcgc cagcacactg gcatggccct gttgtgaaaa gttgatgtgc
     2521 tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg cgatcgcctg ccacaaagcg
     2581 gcgaaatcag cctgctgcca gacagttgca ctggggttat gcggagtgtt gaggatcacc
     2641 agtctggtgc gctcgcttaa taatgcggca aattcctgcc agtcaacgcg aaaatgcggt
     2701 ggttgcagtg ccatacgctt cactattccc ccagaaagcg cgatggcggg ggagtaactg
     2761 tcatagctgg gatcaaaaca aatcacttca tcgccattgc gcaccagtgc ggtaatcgcc
     2821 gcgtataacg cttccgtcgc ccctgccgtt acggtgatat cgctatcggc atctggttga
     2881 tagccataca aacgttccgt tttctgagca atcgcctcgc gcaaggcctg cacgccggtc
     2941 atgggcgcgt attggtttgc cccctgggca acgtggtacg ccagccgctc ctgtaaatag
     3001 cgcggaccat caaaatcagg aaagccttgc gacaggttaa tcgcctggtg ttgctgcgcc
     3061 agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg gaagtttgct ttgtggaatc
     3121 agagggttat ttgtcatttt tattgtacct gacgaagttg cgtgttgaag gcactataac
     3181 acgatgctag tatttggcaa tcaagacgtt tagatgtcta aataaaacaa taaggacaac
     3241 acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc ggctaactac ttttcacatc
     3301 caggaagttt caatcacctg cacgattttt tcactgatga acaactttct cgcgcggtgt
     3361 ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa acttccgcca gcgtttggac
     3421 ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag cgaaagcgat gtacagcaac
     3481 tggcggctga gtccggtgac gatcgcagcg tggtgattgg cgtcggcggc ggcgcactgc
     3541 tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc gtttgttgcc gttccgacga
     3601 tcgccgccac ctgcgccgcc tggacaccgc tctccgtctg gtataacgat gccggacagg
     3661 cgctgcatta tgagattttc gacgacgcca attttatggt gctggtggaa ccggagatta
     3721 tcctcaatgc accgcaacaa tatctgctgg cggggatcgg tgacacgctg gcgaaatggt
     3781 atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc gctaaccgtg cgactgggga
     3841 tcaataatgc gcaggccatt cgcgacgtat tgttaaacag tagcgaacag gcgctgagcg
     3901 atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt ggtggatgct attattgctg
     3961 gtggtgggat ggtcggtggt ctgggcgatc gttttacgcg tgtggcggcg gctcatgccg
     4021 tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt tctccacggc accaaagtcg
     4081 cttatggcat tctggtacaa agcgccttgc tgggtcagga tgatgtgctg gcgcaattaa
     4141 ccggggcgta tcagcgtttt catctgccga ccacgctggc ggagctggaa gtggatatca
     4201 ataatcatgc ggagatcgac aaagtgatta cccacaccct gcgtccggtg gaatccattc
     4261 attacctgcc agtcacgctg acaccagata cgttgcgtgc agcgttcgaa aaagtggaat
     4321 cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc cgccttttcc accgtagcgc
     4381 gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg gcgtttgatc gggatggtta
     4441 acccgcatat gttcgacata gttgtcgtaa tcaggcatac caatcatcag cttcgccgcc
     4501 tgacctaaat attttccggc ttttgccagt gaatcaaaca tcttggcctc cgctaaccat
     4561 gttgctgaaa gagtgccgga tgcggcgtga acgccttatc cggcctacaa atgctatcaa
     4621 cgcgctcggt cagtcccctc gccccatcgg ggagagggtt agggtgagga ggaaccctcg
     4681 gcactatgcc aacattcgcc aacatccccc ctcactctga ctttagtgtg cgccttttgc
     4741 ctgtgccacg atctcctcga cattttccgg cattggctca tacggcgttt ctttcgccgt
     4801 tggcttcgga tctttcaatg ccgccagtgc cgtcttaatc gagaacagcg ccagaaccac
     4861 cacgaccacc ataaagaaga tggttaaccc ggcatccaga cggttgttga acaccagttg
     4921 cgccagctgt gactcagtat actgcgacgg aatattgccg ctgtcgatca ttgcctggaa
     4981 cttattagca atggccagga agccgacttt cgcatccggg ctaaacgctt tctgccagcc
     5041 tgcggtcagg gtacaaatca gcagccaggc cgttggtacc agcgccaccc aggcgtaacg
     5101 ttgacgtttc atcttgaaca acaccacggc acagagcatc agcgccatcc ctgccagcat
     5161 ctggttggca ataccaaaca gcggccacag agtgttaatg ccgcccagcg gatcgaccac
     5221 gccctgatgg aggaagtagc cccacgccag tacgcacagc gctgttgcca gcaggttagc
     5281 aggcagtgaa tcggtccgct tcaggcccgg agaaaccacg cccagcagat cctgcaacat
     5341 aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga ataaacagag cttcaaacag
     5401 aatggcgaag tgataccaga acgccacatc catcatacca cccagcgcac cgtgcaggat
     5461 gtaagccatc cccaccgcca gcgtcggcgc accgcccgca cgggaaatga tcgactgttc
     5521 acccacttcg ctggcaatct ggttgagcgt atctggtgta atgctaaagc cccagctact
     5581 caccacctgc gcggcagaag cgactacatc cgccgtccct gccggagcca gcaccgccat
     5641 tgggctgttc atggcaaaat acacgcccgg atcgatgata caggcagaaa ccagcgccat
     5701 aatcgccacg aaggattcca tcaacatccc accgtagccg ataaagcacg cctgcccttc
     5761 gttcgccagc atcttcggcg tggtgccgga agagatcagc gcatggaagc cagacaccgc
     5821 accacaggcg atggtgataa acaggaacgg gaacaggtta ccggtccata ccgggccagt
     5881 gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc gggcgcataa tcaaaatgcc
     5941 taccgccaga ccgacgatag tcccgatttt caggaaggtc gagagatagt cacgcggggc
     6001 cagcagcaac cacaccggca gcaccgccgc cacaaaaccg tagcccacca gcatccaggt
     6061 cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc ggactttctg ccacccagcc
     6121 gccagagata atggcgaaaa tcaggaacac caggccaatc actgacactt cgccaatgcg
     6181 gcccggacgc agatagcgca ggtagattcc cataaaaatc gccagcggaa tggtgaacgc
     6241 aacggtatac gttccccacg ggctatgagt cagggctttc accacgatca tcgccagtac
     6301 tgcaaggata atgaccatga tcataaagca ggctaccagc gcaatcaccc cggcagttgg
     6361 ccccatctct tctttgacca gctcacccag cgatcgaccg tcacggcgcg tagaaacaaa
     6421 cagcaccatg aaatcctgca ccgcaccagc gagaaccacc ccggcgagca gccagatcat
     6481 ccctggcagg taacccattt gcgccgccag taccggccca accaacggac ctgctccggc
     6541 aatggccgca aaatggtgac cgaacagcac tttcttgtcc gtcggcacat agtccagccc
     6601 gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca accgccagca catttttggc
     6661 gatatacagc ccataaaaac ggtaagcgat cagatagata cagaccgatg ccaccacaat
     6721 ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg tatcccagag caaatgctcc
     6781 cattacagag agcactgtcc agacgaggta tttccctgat ttgttcatag ttgttatccg
     6841 tgtgcgtgtc catagagatg ttacattttg tttctataac atctttgtaa gatttaacaa
     6901 cacggtaacc atgtaaatcg gagttgaaac caatatttaa cttaacaatg ttaactcgat
     6961 cactccgtta accgagtttt cctgcaatct catttaattc tgtcgggctc atcccaaaac
     7021 tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt tcatcgaaaa cgacgatttc
     7081 ccagctctga ttttgccgcc caagatgcag cggctggcag acgccgcgta ccttgccttc
     7141 agacaccggg cgatgatggg tggcgttaag ttccgtaccc accacacact gcccgtcacg
     7201 ggtcatcata aatccggcca tcgatcccag cgtttcagcc agcgccgccg acgcgccgcc
     7261 atgcagcagg ccaaacggct gatgagtacg ggtatcaacc ggcatttcgg cttccagcac
     7321 atcatctccc agacgggtat acacaattcc cagatgcgcc accattgtgt tatcgctggt
     7381 ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc atgcttatgc ccccagcgtt
     7441 gagccgccat cgaccacaat atcctgtagg gtgatatggc tggcgaggtc agaggcgagg
     7501 aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt tccccagcgg aatgccgagt
     7561 ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg cgtcatcgct cacccacagc
     7621 gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca cattacagcg cacgccgcta
     7681 cccgccagtt ccagcccgac gctcaacgcc aggcttttca gcgccgcttt cgatgcgcca
     7741 taagcactca taccaatacg cggcgtgtgt gcggcgtcgg acgccacagt gacaatcgcc
     7801 ccgccccgct gacggcgaaa ctggttcatg gtttgctgga acaggttaaa cgcaccgccg
     7861 acgttaaccg caaaagtctg ctgccagtcc tctttactga gctgatcggt cgcgcccatg
     7921 cgcaaaattc ccgccgcatt gatcagcacg tccagtcgct ccgtttcagc taacagtcgc
     7981 tgacatacct gcgcgacctg cgcagcatcg gcaacatcca tcacttcggt cgcaaaggga
     8041 tattgctcct gagcgaacgc ttgatcaaaa ccggtaactt tcgctcccgc ctcaacaaac
     8101 gccagcgccg tggcgtagcc gataccttta cctgcgccgg ttacccagac atttttaccg
     8161 ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca ccaggcgtcg atggtcgggt
     8221 ttttcgccag catgacaaag tcgatatcac catgcacttt gcgccagcgc gccgccagcg
     8281 ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt gtcgtcatcg aacggttcat
     8341 cggactcgtc cagcaacggc aggataacct cacgcagcgc cgctttgctg gcggggacag
     8401 gtgctggcag taattcttca gtcatcacca cccggccaga ccgcccggcc acatatttca
     8461 gcgacatcaa atgctcgtca cggctgaaat cggccagcgc atccgccacc ataaacggtt
     8521 taatatcgcg cataaatgcg tcggttgcgg tggtcataca gccaatgtgg gcatataccc
     8581 cggtaataat cagctggtta cgtccactct ctttgagcat ttgctccagc ggagaacgat
     8641 gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc ggcatctggc gtcaggcgat
     8701 ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg cccccacata tcattcaaca
     8761 gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt gtaataaacc ggaatattgt
     8821 gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat cacctgctcc atcatcgggc
     8881 agttctcgcc ccagaagctg acaaaatagt cctgcatatc atggattaac aacgcggcac
     8941 gttgcggttc aaaggcccag tcaactttat tcttagggat atcgtgagac tccggcagtg
     9001 cgtaagcctg taattttgga atagccatcg tgttctctcc ttcaggctga tgcgcgtgac
     9061 gccagccact gacgtaattg ttttttatcg actttcccga ccgccgtcag cggaagtgaa
     9121 tctacacact ccacgcgatc cggtaattta aattcggcaa taccctgttc acgcaggaaa
     9181 cgacgcacct gcaccgcgcg cagcggctct tttaccacca gataagcgca gcttttttcg
     9241 cccatcagct catcttccat gctcaccagt gcggcgtaga tcaccgccgg gtggcgcagc
     9301 agcaggtttt cgatctcttc ggcagcgatc ttctcaccgc cacggttgat ctgatctttc
     9361 tcgcgcccct gcacggtgat gtacccctct ggatcaatag agatcagatc gccggaacag
     9421 taaaaaccgt tggcatcaaa ggcgctggca ttgtgctgcg ggcttttgta atagccacgg
     9481 aaggtgtacg gaccgcgggt catcaggcgt ccaacttccc cttgcggcag tggatttcct
     9541 tcggcatcgg caacccacac ttcatcgtcc ggacacattg ggtaaccctg ggtatggata
     9601 attttctccg cgctatcatc aagacgggtg tagttcacca gcccttccgc catgccaaac
     9661 acctgctgca actggcagcc aatctcagcg ggaatacgcg ccgcaagggt ggcagaaaga
     9721 cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct gcgcccggct ttcaccttcg
     9781 gtcagcgcct gcaaccacag gctgactgcc ggtggcacca gcgcggtgac gttcacctga
     9841 tgtttttcaa tcaatgggaa gcaaagcgtg gcgctgggat cggccgccag aacaaccgtt
     9901 cctccggcaa gaaagacgcc cagcgatccc ggcgaactca tggcgtagtt atgagccgcc
     9961 gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac aaatctcgac gctacgacgc
    10021 acgctgtagt agtagtcgtt atgagtgcgc gggatcagtt tcggtgtgcc ggtggtgccg
    10081 ccggaaagct ggaaataggc cacttcatca gcaggtgatg gcgtggcagt aaaatcctca
    10141 gccggatggt taatcgcatc ctgcaagtta tgctcaccgc tgtcgttgtg cagttgcacc
    10201 acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga aatcatcccc gctaaacagc
    10261 gcatgttggc gatcggcaat cagcaatgcg ggttcaatct ggctggcata ggcgttcagt
    10321 tcactacgct gatggctgaa cagcgccagc accggcgcaa cgcccagttt cagcagcgcg
    10381 aaaaaggtaa tatacaattc agcgacgtta cccagttgta ccagcgcggt ttcaccaggt
    10441 ttaatgccct gacggcgtaa actacacgcg aggttatccg ccgcctgatt cagctcccgg
    10501 taactcaact gtcgctcgcc gtcgataacc gcgatgctgt cactcgcagc gtggcgagtc
    10561 agaatgtcgg tcagcggcaa atcctgccag tagccttttt cccgatagcg acgggcaaac
    10621 tcttccggcc agcgggtgaa tggaatgctc atcctcgctc cttaatgcaa tccaaaaacg
    10681 ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact cacccaacgg tgacgacgca
    10741 ggcacaatcc ccgctccggc aaacagacgc acctgatttt cccgcagctt cgcgcagcgg
    10801 atggtcacca cccattcgcc gttaccttcg ctgtcacacc aacccacaat gccgccaaac
    10861 agttcgcggt cgaatggctc cagttcagca ataacctggg tcgcggcctg atgcgggaaa
    10921 ccgctcagcg cgggggttgg atgcagcaga caggccagag tcagtgcgtt ttcttgcgaa
    10981 ttcgctttac cttcaaaggg agttgcgaga tgccacagcg tcggcgtggt aatcaattgt
    11041 ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca gtacctcttt catcgcctga
    11101 gtcaccagtt catgttcatg gcgatctttt tctgacgcca gcagacgatt acccgcttcg
    11161 cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg ctaacggaat ggagctaaaa
    11221 cgctcgccgt ctttacgtag cagcagttcc gggctggccc ccagcaggac gccaccatca
    11281 gccagcggga catggaagtt gtaactaacc gggttttgcg caatcaaccg ttccagcaat
    11341 acgccactat caatggcggc gtcagtggtg atatcaatca atcgtgacaa caccactttg
    11401 tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa ccatctgttc aaacgtggtt
    11461 tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact ggctgcgggt aaaacggcgg
    11521 gctgaggtct gtttttcctg gcgggagaac gactgccagg attcgggaat atacagcgac
    11581 gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca ccggattttt gatgccctgc
    11641 gctttggcat cggcaaacag cgcggcgagt ttttgctgga agggactgtc gggcgaatcc
    11701 ccgttcacag ccggttcatc gaagcgggcg aaacatcctg acgtcgtaaa actgcggtac
    11761 ggcgacataa agaaaaagcg attgggcgca agtgttgcca tggtctgctg tacttcctca
    11821 gccagtgacg tatccatatc atcctccaca aaatgataaa ggctttaata atgattatca
    11881 tttatatttt cggtcgctaa cctaaaagca aacgcgcact atgtcaactc ttgaggtaac
    11941 gcaatctgca actcggggtt gcatctggtt gtggttacaa tgaaaatgag aagcattatt
    12001 gatggattcg cataagcgca atgtgatgtc ctgcgccgtt ctgccccctc tcccttccag
    12061 ggtgagggct ggggtgaggg ttaatgttcg caccagtgct ggctgttccc ctcaccctct
    12121 ccccaaaggg gcgaggggac ggattgtgcg ctttgtcgaa tttgtcatta cgcccataac
    12181 cttattaata acaggaagct gatttgtgag actcgccccg ctctaccgca acgcccttct
    12241 attaacagga cttttgcttt caggaatagc cgcagttcag gccgctgact ggccgcgtca
    12301 gattactgac agccgtggca cacatacact ggaaagccag ccgcagcgta ttgtttccac
    12361 cagcgtcacc ctgaccggct cactgctggc gattgatgct ccggtgatcg ccagcggcgc
    12421 gaccacgccg aataaccgcg tcgcggatga ccagggcttt ttacgccagt ggagcaaggt
    12481 ggcgaaagaa cgcaaactgc aacggctcta tatcggcgaa ccgagcaccg aagccgttgc
    12541 cgcgcaaatg ccggatctga ttttaattag cgcaaccggc ggggattcgg cgctggcact
    12601 gtatgatcag ctttccacca tcgccccgac attaatcatc aattacgacg acaaaagctg
    12661 gcagtcgctg ttaacgcagc ttggcgaaat taccgggcat gagaaacaag cggcagagcg
    12721 gattgcacag tttgataagc aactggcagc ggcgaaagag caaatcaaat taccgccgca
    12781 gccggtcact gccattgtct ataccgccgc tgcacacagt gccaatctct ggacgccgga
    12841 atcagcacaa gggcagatgc tggaacaact cggctttacg ctggcgaagt tgcccgcagg
    12901 gttaaacgcc agccaaagcc agggcaaacg ccatgacatc attcagcttg gtggggaaaa
    12961 tctggctgct gggttaaatg gcgagtcact attcctgttc gccggtgatc agaaagatgc
    13021 cgatgcgatt tatgccaatc cactgctcgc gcaccttccc gcagtacaaa acaagcaggt
    13081 ttatgcgctg ggaaccgaga cgttccgtct ggattactac agcgccacgc aagtgctgga
    13141 acgacttaag gcgctgtttt aagcattaac tgtcagacgc tgtcacctgc ggcggcgtct
    13201 ggcgaaaacg tcgcaactcc accagcacca gcaataacaa cacgccgata atcaacaaac
    13261 caaaaccgct tgcgcttgcg gaagcaaccg gtgtcatcat cgcgcccaaa ccacccagca
    13321 gcgccgcgcc tatggcatcg cccgtcacgt tctgcgccgt ccacaaaccg ttaatccgcc
    13381 ctaacatcgc ttccggggtt tgcgtttgca gcattgtata ttgcagcaac gaactaaccg
    13441 cgctcagcca gccgaacagc gccagacaaa ccacgcctaa aatccacatc ggcatcaggc
    13501 caaacagacc aatggcgagg aacgatccca gcgtggagag cagcatcaat aaccctggtc
    13561 gcgcactatg tgccagcttc ccgctggtta acgcaccaat agccgcgccg agcgggatcg
    13621 ccgcgtagag aaaaccaatc tgtgccgctg acatctgcca gttgtcagcc agcgccggat
    13681 acagtacccg caccgcgctc gccatcgtca ataaaccacc cagcagcgca atcccgccca
    13741 ccagcgggct ggcaagcaga aaacgaaatc ctgccagtaa tgatttcaac ggatgctcac
    13801 gcggctgcgg tggcggtggc aacgccggaa ggcttaacaa cggtagcaag gtaataaacg
    13861 tgcccgccgc cgccagcccg tagttccagg ctacgccacc ggtcgccagc aataaaccgc
    13921 caatcatggg cgaaatcacc gaccccagac gcacggtcaa catggtgatc gccccggcct
    13981 gcattaagtt ttcacgccct accagtgctg gtgtcgccgc caatagcgcc gtaacgccaa
    14041 gcgatgcgaa aaaaccatcc cataaaccaa gtaaatagat tgccagcaat gacggctccg
    14101 gcagcagtgc attaaggcac agtccaatga agccaatgcc acaggtgccg cgcgccagca
    14161 aaatcacttt tttgcgctca tagcgatccg ccagcacacc gccaaccatc aggccaacaa
    14221 acatcgcgcc gccggtcagc gtcaccgaaa gccccacctg ccaggtagaa tgcgtcatca
    14281 tctggatctg caccggcacc gcgacgccga gcaaacccag agacacaatt gagataaaac
    14341 gagcgaggaa tactgcgcga aacgccgggt gcgttttcaa caggctgagg ttaagcagcc
    14401 aggattgttt attcattaca atgccttgcc atcaaatatt ttaaatacct attcttaggc
    14461 tgcacatgct aacatatcca aataagatag ataacgataa ttaatttcat tatcatggaa
    14521 gttcgtatgt ctggttctgt tgccgtgaca cgcgccattg ccgtgcccgg attgctgtta
    14581 ttactgatta tcgcgacggc attaagcctg ctcattgggg caaaatcact ccccgcttcc
    14641 gtagtgctgg aggccctctc cggcacctgc cagagcgccg actgcaccat cgtgctcgac
    14701 gcgcgactgc cgcgcaccct tgccggttta cttgcaggcg gcgcgcttgg ccttgccggg
    14761 gcgttgatgc aaaccctcac ccgaaatcca cttgccgacc ccggcttgct tggcgtgaat
    14821 gccggagcca gctttgccat tgtgctgggt gcggcgctgt ttggttactc atccgcgcag
    14881 gaacaactgg cgatggcctt cgccggggcg ctggtggcct cattgattgt tgcctttacc
    14941 ggcagtcagg gcggcgggca gttaagtccg gtgcgtttaa ccctggcggg cgtggcgctg
    15001 gcggcggtgc tggaagggct gaccagcggc atcgccctgc ttaatcctga tgtctacgat
    15061 caactgcgtt tctggcaagc cggttcgctg gatattcgca atctacatac cttaaaagtg
    15121 gtgctgatcc cggtgctgat cgccggagca accgcgctat tactgagccg cgcgctgaac
    15181 agtttaagcc tcggcagcga caccgcgacg gcgctgggca gtcgcgtggc gcgcacacag
    15241 ttaattggtc tgctggcgat taccgtgctt tgtggtagtg cgacggcggt agttggcccg
    15301 attgccttta tcggcctgat gatgccgcac atggcgcgct ggctggtagg tgccgatcat
    15361 cgctggtcgc tgcccgtcac gctacttgct acccctgccc tgctgctgtt tgccgatatc
    15421 atcgggcggg tgattgtgcc cggcgaactg cgcgtttctg tggtcagtgc gtttattggt
    15481 gcaccggtgc tgatcttcct cgtgcgacgt aaaacgcgag gtggcgcatg atttacgtct
    15541 ctcgccgatt aatcatcacc tgtttgctgt tggtttccgc ctgtgtggtt gcaggtatct
    15601 ggggattacg cagcggtgcc gtcacgctgg aaacctcgca ggtattcgcc gcgctgatgg
    15661 gcgatgcgcc gcgcagtatg acgatggtgg tcaccgaatg gcgtttacca cgcgtgctga
    15721 tggcgctgtt gattggcgcg gcactgggcg tcagcggcgc gatttttcag tcgctgatgc
    15781 gtaacccgct cggcagccct gacgtaatgg gctttaacac cggggcgtgg agcggcgtgc
    15841 tggtagcgat ggtgctgttt ggtcaggacc tgacggctat cgcgctggca gcaatggtgg
    15901 gcggcattat cacttcgctg ttggtctggc tgctcgcctg gcgtaacggc atcgacacct
    15961 ttcggttgat tattatcggt atcggcgttc gtgccatgct ggtggccttt aatacctggc
    16021 tgttgctgaa agcgtcttta gaaacggcgc taacagcggg tttgtggaat gcaggatcgc
    16081 tcaacggcct gacgtgggca aaaacctcgc cttccgcacc catcattata ttgatgctca
    16141 ttgccgccgc cttactggta cgccggatgc gcttgctgga aatgggcgat gataccgcgt
    16201 gtgcgctggg cgtcagcgtc gaacgttcgc gtctgttaat gatgctggtt gcagtggtgc
    16261 ttaccgctgc ggcaaccgcg cttgccgggc cgatttcctt tattgcttta gtcgcgccgc
    16321 acattgcccg acgcattagc ggcaccgctc gctgggggct gacccaggcg gcgctgtgcg
    16381 gggcgctgtt actgctggcg gccgatctct gcgcccaaca actgtttatg ccgtatcaac
    16441 ttccggttgg cgtcgttacc gtcagcctcg gcggtattta ccttatcgtc ttgttaattc
    16501 aggagtctcg caaaaaatga acgaatctgt agcccgtttg cgcggcgaac agttaaccct
    16561 cggatatggc aaatataccg ttgcggaaaa tctgactgta gaaatacctg atggtcactt
    16621 cacggcaatt atcgggccaa atggctgcgg taaatccact ttactgcgta ctttaagccg
    16681 cctgatgacg cctgctcacg ggcatgtctg gctggatggc gagcacattc aacattacgc
    16741 cagtaaagag gttgcacgcc ggattggtct gttggcgcaa aatgctacca cgccaggcga
    16801 tatcaccgtg caggagctgg tggcgcgtgg gcgttacccg catcaaccgc tatttacccg
    16861 ctggcgcaaa gaagatgaag aagcggtaac gaaagcgatg caggccacgg gaataactca
    16921 tctggcagat caaagcgtgg ataccctttc tggcggacaa cgccagcgcg cgtggatcgc
    16981 gatggtgctg gcccaggaaa cggcaattat gctgctcgac gaacccacga cctggctgga
    17041 tatcagtcat cagattgatc tactggaact gttaagcgaa ctgaaccgcg agaaaggcta
    17101 taccctggcg gcggtgctgc acgatcttaa tcaggcctgt cgttacgcca gccatttgat
    17161 tgcattgcgg gaagggaaaa ttgttgctca gggagcgccg aaggagattg tcactgctga
    17221 actgattgag cgcatttatg gtctgcgatg catgatcatt gacgatccgg tagccggaac
    17281 gccgcttgtg gtgccgctcg gacgaacggc accttcaacc gcaaatagtt aaactaagtg
    17341 gtctgccatc atggcatcct gttttctgga tgccatcgca tagcgcaata acacgctacc
    17401 acaagccacc atcccgccga tcagcgcgga aaggatcaca ataatcgcct tacccggacc
    17461 gtcttttttc actggcaatg acgggcttaa ctgatattta aacggcgtaa aattcacatc
    17521 gttgatattt gcttttgtta actgctcaac aagatactga cgattacgta attcaccgtt
    17581 cagttccgca acgtcagtga ccgctttttc aatttccagt ttgcgttcaa taccgtctgc
    17641 gccgagagaa atagagaaat cggggtcatc tttaacggcc tgaccattac tgtatacggg
    17701 ctttttaatt cccgccgcgt tggcaatgtc gagtgaataa ttgaggcgct gaatgtttgc
    17761 atcaagttga tttttcgttt taatgcgatc ctgagccagt ttttcttttt caaactgggt
    17821 tttgatttcc agtttattac ggacgttttc tagtgactct ttcaccacca acgcagagat
    17881 ataatcgata tacccgctca aaacggtctg cgcctcttca ctggttggcg cggtaaaact
    17941 tagcgtccag gaggtataca gtgacggttc atcttttttc ttactggcat tgtcatcaac
    18001 cgctttcatt ttttcgctca atgcaacaat agcgcgatgc aaatccagtt catcgatttt
    18061 cgcctctttt aattggtcca tcacataagg tgatgaacgc aggtactctt ccagcaagct
    18121 aaccgactga aacttcttga taaacaggtt aaatgcttct gtacgatcaa ttttgatatc
    18181 cagatccagc actcgaagct tagtgaatgt tttctccagc tcttgccact gaacaggttc
    18241 tggaggcgtg acaaccgccg cgctggtcca tttttgcggc aggatgaaag agatcagcaa
    18301 gcctgcgcag gcaaacgcaa aaatgaccgc catgaccgtt tttttggccc gccataaaac
    18361 tgcgattaga ttaagtaaat caatttcatt attactgggc gacgccagag gatactcagg
    18421 aaaatgagcg tcacttccct gtttaatatt cagtgatgac atggggtata acctgaaaat
    18481 gagtccaata ggggaaatct ccacggcgct cactttacca gttggtgata attatccgaa
    18541 gctgaagttt gtaaattcct tccactga
//
LOCUS       DAZFJF010000092        18341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFJF010000092 DAZFJF010000000
VERSION     DAZFJF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.092"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..582)
                     /gene="rsmG"
                     /locus_tag="ACHKAU_003471"
     CDS             complement(<1..582)
                     /gene="rsmG"
                     /locus_tag="ACHKAU_003471"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO5532467.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALD"
     gene            complement(646..2535)
                     /gene="mnmG"
                     /locus_tag="ACHKAU_003472"
     CDS             complement(646..2535)
                     /gene="mnmG"
                     /locus_tag="ACHKAU_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO5532468.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNVSQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2913..3356)
                     /gene="mioC"
                     /locus_tag="ACHKAU_003473"
     CDS             complement(2913..3356)
                     /gene="mioC"
                     /locus_tag="ACHKAU_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO5532469.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3446..3904)
                     /gene="asnC"
                     /locus_tag="ACHKAU_003474"
     CDS             complement(3446..3904)
                     /gene="asnC"
                     /locus_tag="ACHKAU_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO5532470.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4056..5048
                     /gene="asnA"
                     /locus_tag="ACHKAU_003475"
     CDS             4056..5048
                     /gene="asnA"
                     /locus_tag="ACHKAU_003475"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO5532471.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5053..6504)
                     /gene="viaA"
                     /locus_tag="ACHKAU_003476"
     CDS             complement(5053..6504)
                     /gene="viaA"
                     /locus_tag="ACHKAU_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO5532472.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6498..7994)
                     /gene="ravA"
                     /locus_tag="ACHKAU_003477"
     CDS             complement(6498..7994)
                     /gene="ravA"
                     /locus_tag="ACHKAU_003477"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO5532473.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8217..10085
                     /gene="kup"
                     /locus_tag="ACHKAU_003478"
     CDS             8217..10085
                     /gene="kup"
                     /locus_tag="ACHKAU_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO5532474.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10252..10671
                     /gene="rbsD"
                     /locus_tag="ACHKAU_003479"
     CDS             10252..10671
                     /gene="rbsD"
                     /locus_tag="ACHKAU_003479"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO5532475.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10679..12184
                     /gene="rbsA"
                     /locus_tag="ACHKAU_003480"
     CDS             10679..12184
                     /gene="rbsA"
                     /locus_tag="ACHKAU_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO5532476.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12189..13154
                     /gene="rbsC"
                     /locus_tag="ACHKAU_003481"
     CDS             12189..13154
                     /gene="rbsC"
                     /locus_tag="ACHKAU_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5532477.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13179..14069
                     /gene="rbsB"
                     /locus_tag="ACHKAU_003482"
     CDS             13179..14069
                     /gene="rbsB"
                     /locus_tag="ACHKAU_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO5532478.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14195..15124
                     /gene="rbsK"
                     /locus_tag="ACHKAU_003483"
     CDS             14195..15124
                     /gene="rbsK"
                     /locus_tag="ACHKAU_003483"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5532479.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15128..16120
                     /gene="rbsR"
                     /locus_tag="ACHKAU_003484"
     CDS             15128..16120
                     /gene="rbsR"
                     /locus_tag="ACHKAU_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO5532480.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMMPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16086..17513)
                     /gene="mdtD"
                     /locus_tag="ACHKAU_003485"
     CDS             complement(16086..17513)
                     /gene="mdtD"
                     /locus_tag="ACHKAU_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO5532481.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17536..18228)
                     /gene="yieP"
                     /locus_tag="ACHKAU_003486"
     CDS             complement(17536..18228)
                     /gene="yieP"
                     /locus_tag="ACHKAU_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO5532482.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4415 a         4546 c         4791 g         4589 t
ORIGIN      
        1 atccaaggct ggaacctgaa gtttaaccac tgattcgacc tgatattctt cgggcaacaa
       61 agcgatttca tcttccggca tttgcccttt cagcgcgtag aaacggcctt gctcaccagg
      121 aagatggtgg caccagctca ccatatcgtt cagagaggca aaagcgcggc taattacgcc
      181 atcaaatggc ggctctgaag gaaactcttc taccctgctc tgtactggtt caatattctc
      241 cagtttaagc tcatgttgca cctgacgaag gaaacgcacg cgtttaccaa ggctatccaa
      301 cagagtgaaa tgggcttcag gacgcacgat agagagtgga atgcctggca gtcctggtcc
      361 ggtgccgaca tcgataaacc gttcaccttg cagatacggt gccaccacaa tgctatcgag
      421 aatatggcgt accagcatct cattaggatc gcggaccgaa gtcaggttgt acgctttgtt
      481 ccatttatgc agcatattca cgtaggcaat aagctggttt ttctggtgat cggtaagcga
      541 aatacctgcg tctttcagca gtaaggagag tttgttgagc acggtgatta cctgttcttg
      601 atgcgttgcc tggtaagcgg gtgcttacca ggcattttta atgcgttatg cgctacgacg
      661 cagcataccc tgttttttca gccacaccag cagaatggag atggccgcag gcgtgacgcc
      721 agaaatacgc gaagcctggc cgatagaggc tggtttgtga tcgttaagtt tggcgatcac
      781 ttcgttagaa agaccggata cctggcggta atccagtgtc gcgggtagca gggtgttctc
      841 gttacgcagc tgcttttcga tctcatcttg ctggcgcgcg atataacctt cgtatttaac
      901 ctgaatctca acctgttccg ccgcctgttc gtctgtcaac gcaggggcaa acggcgtcag
      961 cgtggttaat ttttcataag tcatttccgg acgacgcagc agatcttcac cactggcttc
     1021 acgggaaagc ggcgcagtca ggtgagcatt cacttcggct gcagcttccg ccgacggggt
     1081 tacccaggtc gatttcagac gctgacgctc acgctcgata ttctcaagtt tctcgttaaa
     1141 gcgcgcccaa cgttcgtcat ccaccaggcc cagttcacga ccgatttcag tcaaacgcag
     1201 atccgcatta tcttcgcgta gcatcagacg atattctgcg cgcgaagtaa acatacgata
     1261 cggttctttg gttcctaaag tgcacaggtc atcaactagt acgccgagat acgcctgaga
     1321 acgtgccgga gcccaacctt ctttgtcagc agacagacgg gcagcgttaa gaccggccag
     1381 caaaccttgc gcagcggctt cttcgtaacc ggtagtgccg ttaatctgac cagcaaagaa
     1441 cagcccctgg ataaacttgc tctccagcgt cggtttcagg tcgcgaggat cgaagaagtc
     1501 atactcaatg gcataacccg gacgcacgat cttcgcgttt tccatcccct gcatagagcg
     1561 gacgatttgc atctgcacat cgaacggcag gctggtggag ataccgttcg gataaatttc
     1621 attagaggtc agtccttccg gttcaaggaa gatctgatgc tgatttctgt cggcgaagcg
     1681 cataactttg tcttcgatcg acgggcagta gcgtgggccg acaccttcga tcacccctgc
     1741 atacattggg ctacgatcga ggttactgcg gatcacatca tgggttttct cgttggtatg
     1801 agtgatataa cacggcacct gctggggatg ctgggacaca ttgcccataa acgagaatac
     1861 cggcattggg ttatcgccat gctgttgcgc cagcacacta aagtcgatag ttcgagcatc
     1921 aatacgcggt ggtgtcccgg ttttcagacg accaacgcgc agcggcagtt cacgcaaacg
     1981 gcgagaaagc ggaatggacg gcggatcacc agcacggcca ccgctgtaat tatccagacc
     2041 gatatgaatt ttaccgtcga ggaacgtccc aacggtgagc acgacggctt tggcacggaa
     2101 cttcagtccc atttgggtaa cagcaccgac cacgcgatcg ttttcgacaa taagatcttc
     2161 aaccgcctgc tggaagatca tcaggttcgg ttggttctcc agcgccgtac gtaccgcctg
     2221 acggtagagc acacgatccg cctgagctcg ggtagcgcga accgccggtc ctttgcttgc
     2281 gtttagtatc ctaaactgga tacccgcttg atcgatcgct ttcgccatca gaccgccgag
     2341 tgcatccact tcttttacca gatgtccctt cccaataccg ccgatcgccg ggttgcagct
     2401 catctgcccc agagtgtcga tattgtgtgt caaaagcaga gtctgttgac ccatacgcgc
     2461 cgcggccatc gcggcctcgg tgcctgcatg acccccgcca atgatgatga cgtcaaaagg
     2521 atccggataa aacatggtga ttgcctcgca taacgcggta tgaaaatgga ttgaagcccg
     2581 ggccgtggat tctactcaac tttgtcggct tgagaaagac ctgggatcct gggtattaaa
     2641 aagaagatct atttatttag agatctgttc tattgtgatc tcttattagg atcgcactcc
     2701 ctgtggataa caaggatccg gcttttaaga tcaacaaccg ggaaaggatc attaactgtg
     2761 aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta tacacaactc
     2821 aaaaactgaa caaccgttgt tccttggata actaccggtt gatccaagct tcctgacaga
     2881 gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat ccacgatccc
     2941 agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt
     3001 tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc gatagcccca
     3061 caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac cgcagaaaga
     3121 tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg aatatctccg
     3181 gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc ttccaacagc
     3241 ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc caggtgttct
     3301 gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc tgccataaac
     3361 cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat ctacctgtgg
     3421 aaaagtatgg gattaaaaaa gccgattatg gcttgatggt acgcatgatc gggttttgca
     3481 ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg ttgataagta
     3541 catgctggag agcgtcgatc gaacggcaca tcacttttat aaagatgctg tagtggccgg
     3601 ttgtgtaata ggcttcagtg acttcatcaa ggctttccag ctttgccagc gcggaagggt
     3661 agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa ccgagctgct
     3721 tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt ttctctactc
     3781 gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg taagcggtgc
     3841 gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc agataatttt
     3901 ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct tttttaatga
     3961 atcaaaagtg tgttaggctt tttattgaat gattattgca tgtgtgtcgg tttttgttgc
     4021 ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac attgccaaac
     4081 aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa cgtctggggc
     4141 tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag gataacttgt
     4201 cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc cagttcgaag
     4261 tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg
     4321 gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagagcgt ctttctccgt
     4381 tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac ggtgagcgtc
     4441 aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa gcaaccgaag
     4501 ctgcagttag cgaagagttt ggtttggcac cgttcctgcc ggatcagatc cacttcgtac
     4561 acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt gagcgggcga
     4621 ttgcgaaaga tcttggcgcg gtattcctcg tcgggattgg cggcaagctg agtgatggtc
     4681 atcgtcacga cgtgcgcgca ccggattatg atgactggag caccccgtca gagctgggcc
     4741 atgcgggtct gaacggcgat attctggtat ggaaccctgt actggaagat gcgtttgagc
     4801 tttcttccat ggggatccgc gtggatgccg acacgttgaa gcatcagttg gcgctgaccg
     4861 gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt gaaatgccgc
     4921 agaccatcgg cggcggtatc ggccagtctc gtttgaccat gctgctgctg caactgccgc
     4981 atatcggcca ggttcagtgt ggagtatggc cagctgctgt tcgcgagagc gtcccttctc
     5041 tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa
     5101 gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg acatcgccac
     5161 ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg tcacgtcgtc
     5221 aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa accattccct
     5281 gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc cgccacgaaa
     5341 ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa gctcataacg
     5401 gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga gagcaatgcg
     5461 catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca ttgagccgga
     5521 agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt tatgtaccac
     5581 cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg tgagcaactg
     5641 tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta gtgtcgccag
     5701 ttctggcggc agaagacgta aaatatcatc gctttgttgc agaccatcaa cctgctcagg
     5761 aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat cgttgcgcgg
     5821 tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca gttccggctg
     5881 ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt taagctgacc
     5941 ggcgctcata tcccacagac gaccagctgc agtattgtta tctgcgagaa tcggttcaag
     6001 ttgtccgctc agcgtcatgc gttcctgaac ttcactcaac agttgttcgc gttcttcttc
     6061 taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc aacgctggag
     6121 aaaaagcgtg tgtaacgctg aagtgatcgt gctgttagca tcaaccaact gtcgggcttg
     6181 ttctgcccat ggagaattca gacgatgcag taagtccagg atctgtggta gctgcacaat
     6241 aaactgtggc gtggagagga gctggctttg ctgatagcac atcacctctt cggtgagttc
     6301 cggcgggact cgggcatctt tcagccgact gcgcagcgct tcacgccagc ggggaacatc
     6361 atcagtgatt gccgccttca ggcgtgggaa tttttcaaag aagaccgcca gctgcggtga
     6421 ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg ccagcatcac
     6481 attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcacc gacatcctgt
     6541 aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt ttgctgttcg
     6601 ctgaataaag cgtgttgctt gcgccagtcg ctttcaagct cttccagttg ttgttttatt
     6661 tcaccaggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc ttgtaaactt
     6721 acatcgcgaa cgacaagatg ttgggcgcta tcaacttcca gattcagttt ctgggcaaag
     6781 ccgataccgt tcagtttacc gcgaatttca ccacctttgc tcagccactg ttccagcgcg
     6841 ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg tttttgcagc
     6901 agtagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg acgacggctg
     6961 aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg ctgctggagt
     7021 tgcaggtgac gttgcacaat cgcgccaagg cgagtcaaca tcccttgctg ttgccaggcg
     7081 tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac
     7141 cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg accgctaaaa
     7201 aaggcgctgg cctgcaataa tcggatcgct tttttccagc gacgatccga gacataaggc
     7261 gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa tacatgatcg
     7321 ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc tgtgacctgc
     7381 aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat ggagcggaaa
     7441 ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc atataacgct
     7501 tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag acgcatcggg
     7561 attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt gagcaaggta
     7621 ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc tgcttccggc
     7681 aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg
     7741 ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa cgcgcgggca
     7801 ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc tggcgggcca
     7861 aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat ggcgtggcta
     7921 cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc cgctaataaa
     7981 tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt
     8041 atagacgttt cttgctggtg gctaaaatag tctcaaaggg ggggtatttt tctttgagcc
     8101 aggttaatgt ggccgcattt aggagtacga ttttgccgtt aatcgtgcat actgtgcgct
     8161 tttttgtggg ccaagggact aagcacacat ttcatatttc aacgaaagac tagtctatga
     8221 gcactgataa taagcaatca ttgcccgcga ttaccctcgc ggcgattgga gttgtctacg
     8281 gcgatattgg taccagcccg ttatatacac ttcgtgaatg tttgtccggc cagtttggtt
     8341 ttggcgttga acgcgatacc gtgtttggct ttttatcgct gatcttctgg ctgctaatct
     8401 ttgtggtttc cattaaatat ctcaccttcg tgatgcgggc agataacgcc ggtgaggggg
     8461 ggatcctgac gttgatgtcg cttgccgggc gtaatacgtc ggcgcgaacc acatcaatgc
     8521 tggtgattat ggggctaatc ggcggcagct ttttctatgg tgaagtcgtc ataacacccg
     8581 ctatttcggt gatgtcagcc attgaaggtc tggaaatcgt cgccccgcag ctggatacct
     8641 ggatagttcc cctctcaatt atcgttctca ctttattatt tatgattcaa aaacatggca
     8701 cggctatggt cggtaagctg tttgcgccga tcatgctgac ctggtttttg attctggcag
     8761 gactggggtt acgtagcatt attgctaacc cggaagtgct gcatgcgctg aatccaatgt
     8821 gggcggtgca tttcttcctt gaatacaaaa cggtttcttt tattgcatta ggggcagtgg
     8881 tgttgtcgat tacgggggtc gaggcgctgt atgctgatat ggggcacttt ggtaagttcc
     8941 ctattcgcct ggcgtggttc accgtcgtat taccttcctt gacccttaat tacttcggtc
     9001 agggagcgct gttgttaaag aacccggaag cgattaagaa cccgttcttc ctgttggcac
     9061 cggactgggc gctgatcccg ctgctgatca tcgccgcact ggcgacggta attgcctcgc
     9121 aggcggttat ctctggcgtc ttctcattga cgcgtcaggc ggtacgtctg ggatatttgt
     9181 cgccgatgcg cattattcac acctccgaaa tggagtcagg gcaaatctat attccgtttg
     9241 tgaactggat gctctatgtc gcggtcgtga ttgtgattgt cagctttgag cactccagca
     9301 acctggcggc ggcgtacggg attgcggtga ccggaaccat ggtgctgacg tctattctct
     9361 cgactaccgt ggcacgtcag aactggcact ggaataagta ttttgttgcg ctgatcctga
     9421 ttgctttcct ttgtgtcgat attccattgt tcaccgctaa cctcgataaa ctgctctccg
     9481 gcggctggtt gccattgagc ctcggtactg tgatgtttat cgtgatgacc acctggaaga
     9541 gcgagcgttt ccgcttgctg cggcggatgc atgaacatgg taactctctg gaagcgatga
     9601 ttgcttcgct ggagaaatca ccgcctgttc gcgtgcccgg gaccgcggtg tatatgtcgc
     9661 gtgcaatcaa cgtcattccc tttgcgctga tgcataacct taaacataac aaggtattgc
     9721 atgagcgggt gattctgtta actctgcgta ccgaagacgc gccatatgtc cataatgtcc
     9781 gtcgggtaca gattgaacaa ctgtcgccca ctttctggcg cgtggtggca agttatggtt
     9841 ggcgagaaac gccaaacgtg gaagaagttt tccaccgctg cggtctggaa ggattaagtt
     9901 gccggatgat ggaaacctcc ttctttatgt cgcatgagtc gttgatcctc ggcaaacgcc
     9961 cgtggtattt gcgtctgcgc ggcaagctgt acttgctgct gcaacgtaat gcgctgcgtg
    10021 caccagatca atttgaaatt ccgccaaaca gggttatcga actgggtact caggtcgaaa
    10081 tctaacgcca gacgcctcct ttctccataa gggggcgttt ttgttttcat ggttaatcac
    10141 catgtaaaac gtttcgaggt tgatcacatt tccgtaacgt cacgatggtt ttcccaactc
    10201 agtcaggatt aaactgcggg tcagcgaaac gtttcgctga tggagaaaaa aatgaaaaaa
    10261 ggcaccgttc ttaattctga tatttcatcg gtgatctccc gtctgggaca taccgatacg
    10321 ctggtggtgt gtgatgctgg tttacctatc cccaaaagta caacgcgtat cgatatggca
    10381 ttaacccagg gtgtaccttc ttttatgcag gtgctgggcg tcgtcacaaa tgaaatgcag
    10441 gtcgaggctg ccattatcgc ggaagagatc aaacaacata atccgcaact ccacgaaacg
    10501 ttgctcactc accttgagca gctgcaaaaa caccagggaa ataccattga aattcgttac
    10561 accacgcatg aacaattcaa acaacaaacc gcagaaagtc aggcggtaat tcgcagcgga
    10621 gaatgttctc cgtatgcgaa tatcattctc tgtgctggcg tgacgttctg aggccgtcat
    10681 ggaagcatta cttcagctta aaggcatcga taaagccttc ccgggcgtaa aagccctctc
    10741 gggcgcagcg ttaaatgtct atccgggccg cgtgatggcg ctggtgggcg aaaacggcgc
    10801 gggtaaatcc accatgatga aagtgcttac tggtatctat gctcgcgatg ccggcacgct
    10861 tttatggctg gggaaagaaa cgacatttac cgggccgaaa tcttcccagg aagccgggat
    10921 tgggattatc catcaggaac tgaacctgat cccgcagttg accattgccg aaaacatttt
    10981 cctcggtcgt gagtttgtta atcgctttgg caaaattgac tggaaaacca tgtatgccga
    11041 agcggataaa ttgctggcta aacttaacct gcgctttaaa agcgacaagc tggtgggcga
    11101 tctttccatc ggtgaccagc aaatggttga aatcgccaaa gtgctgagct ttgagtcgaa
    11161 agtcatcatt atggatgaac cgaccgatgc gctgaccgat accgaaaccg aatccctgtt
    11221 ccgcgtcatc cgcgaactaa aatcgcaagg ccgcggtatt gtctatatct cccaccgcat
    11281 gaaagaaatc ttcgagattt gcgatgacgt taccgttttt cgtgacgggc aatttattgc
    11341 tgagcgcgaa gtggcatcac tgaccgaaga ttcgctgatt gagatgatgg tgggccgcaa
    11401 gctggaagat caatatccac acctggacaa agcgccggga gatatccgcc tgaaagtcga
    11461 taatctctgc ggacctggcg ttaacgatgt ctcttttact ttacgcaaag gcgaaattct
    11521 tggcgtctct ggtttgatgg gcgcaggtcg taccgaactg atgaaagtgc tctacggcgc
    11581 actaccgcgc accagcggtt acgtcaccct ggatgggcat gaagtcgtta cccgttcacc
    11641 gcaggatggc ctggcaaacg gcattgtgta tatctccgaa gaccgtaaac gtgacggttt
    11701 agtgttgggc atgtcagtaa aagagaacat gtcgctgaca gcgctgcgct acttcagccg
    11761 cgctggcggc agtttgaagc atgccgatga acagcaggct gtgagtgatt tcattcgtct
    11821 gtttaatgtg aaaacaccgt cgatggaaca ggcaattggt ctgctttccg gtggcaatca
    11881 gcaaaaagtg gcgattgccc gcggtctgat gacacgcccc aaagtgttga tccttgatga
    11941 acctacccgt ggcgtagatg tcggcgcgaa aaaagagatc tatcaactga ttaaccagtt
    12001 caaagccgat ggcttgagca tcattctggt gtcatcggag atgccagaag tattaggcat
    12061 gagcgatcgc atcatcgtca tgcatgaagg gcatctcagc ggggaattta ctcgtgagca
    12121 ggccacccag gaagtgttaa tggctgccgc tgtgggcaag cttaatcgcg tgaatcagga
    12181 gtaaaaaaat gacaacccag actgtctctg gtcgccgtta tttcacgaaa gcgtggctga
    12241 tggagcagaa atcgcttatc gctctgctgg tgctgatcgc gattgtctcg acgttaagcc
    12301 cgaacttttt caccatcaat aacttattca atattctcca gcaaacctcg gtgaacgcca
    12361 ttatggcggt cgggatgacg ctggtgatcc tgacgtcggg catcgacttg tctgtaggtt
    12421 ctctgttggc gctaaccggc gcagttgctg catctatcgt cggcattgaa gtcaatgcgc
    12481 tggtggctgt cgctgccgct ctcgcgttag gtgccgcaat tggtgcggta accggggtga
    12541 ttgtagcgaa aggtcgcgtt caggcgttta tcgctacgct ggttatgatg cttttactgc
    12601 gcggcgtgac catggtttat accaacggta gcccagtgaa taccggcttt actgagaacg
    12661 ccgatctgtt tggctggttt ggtattggtc gtccgctggg cgtaccgacg ccagtctgga
    12721 tcatggggat tgtcttcctc gcggcctggt acatgctgca tcacacgcgt ctggggcgtt
    12781 acatctacgc gctgggcggc aacgaagcgg caacgcgtct ttctggtatc aacgtcaata
    12841 aaatcaaaat catcgtctat tctctttgtg gtctgctggc atcgctggcc gggatcattg
    12901 aagtggcgcg tctctcctcc gcacaaccca cggcggggac tggctatgag ctggatgcta
    12961 ttgctgcggt ggttctgggc ggtacgagtc tggcgggcgg aaaaggtcgc attgttggga
    13021 cgttgatcgg cgcattaatt cttggcttcc ttaataatgg attgaatttg ttaggtgttt
    13081 cctcctatta ccagatgatc gtcaaagcgg tggtgatttt gctggcggtg ctggtagaca
    13141 acaaaaagca gtaataacga ctacaggaca tcttgaatat gaacatgaaa aaactggcta
    13201 ccctggtttc cgctgttgcg ctaagcgcca ccgtcagtgc gaatgcgatg gcaaaagaca
    13261 ccatcgcgct ggtggtctcc acgcttaaca acccgttctt tgtatcgctg aaagatggcg
    13321 cgcagaaaga ggcggataag cttggctata acctggtggt gctggactcc cagaacaacc
    13381 cggcgaaaga gctggcgaac gtgcaggact taaccgttcg cggcaccaaa attctgctga
    13441 ttaacccgac cgactccgac gcagtaggta atgctgtgaa gatggctaac caggcgaaca
    13501 tcccggttat cactcttgac cgccaggcaa cgaaaggtga agtggtgagc cacattgctt
    13561 ctgataacgt actgggtggc aaaatcgccg gtgattacat cgcgaagaaa gcgggtgaag
    13621 gtgccaaagt tatcgagctg caaggcattg ctggtacatc cgcagcccgt gaacgtggcg
    13681 aaggcttcca gcaggccgtt gctgctcaca agtttaatgt tcttgccagc cagccagcag
    13741 attttgatcg cactaaaggt ttgaacgtaa tgcagaacct gttgaccgct catccggatg
    13801 ttcaggctgt attcgcgcag aatgatgaaa tggcgctggg cgcgctgcgc gcactgcaaa
    13861 ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg tacaccggat ggcgaaaaag
    13921 cggtgaatga tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga
    13981 aaggcgtcga aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg
    14041 ttgatctgaa actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac
    14101 ataaatacgt catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc
    14161 taacacggtg gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt
    14221 tcttggcagc attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga
    14281 aaccgtaacc ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt
    14341 ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc tgtacgggtg atgacagcat
    14401 tggtgagagc gttcgccagc agctcgccac tgataacatt gatattactc cggtcagcgt
    14461 gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt
    14521 catcggtatt catgccggcg ctaatgctgc cctttccccg gcgctggtgg aagcgcaacg
    14581 tgagcgtatt gccaacgcgt cagcattatt aatgcagctg gaatcaccac tcgaaagtgt
    14641 gatggcagcg gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc
    14701 ggctcgcgaa cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac
    14761 ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc
    14821 gcaggtactg catgaaaaag gtatccgtac tgtactgatt actttaggaa gtcgtggtgt
    14881 atgggctagc gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga
    14941 taccattgct gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa
    15001 accattgcca gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa
    15061 aggcgcacaa ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag
    15121 gtgacgcttg gctacaatga aagatgttgc ccgcctggcg ggcgtttcta cctcaacagt
    15181 ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc
    15241 ggcgattaaa gaactcaatt acgcgccatc agccctggcg cgtagcctca aactcaatca
    15301 aacacatacc attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt
    15361 gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga
    15421 aggagatgaa cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg
    15481 cttgctgtta ctgtgcaccg aaacgcatca accttcgcgt gaaatcatgc aacgttatcc
    15541 gacagtgcct actgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca
    15601 ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac
    15661 ccgtatcgcc tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg
    15721 ttatcgggcg gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg
    15781 tgattttgaa tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg
    15841 tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata
    15901 tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact
    15961 ggcaagcttt atgatgccac cattaaccac tatccaccaa ccgaaagatg aactggggga
    16021 gctggcgatt gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt
    16081 acaacttact ccgattctga tggaacgcgg ttcggcttag atttacgctg tcttttgatc
    16141 aaattattac catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata
    16201 atgcccatcg tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct
    16261 tcataaacgc gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc
    16321 gtgaccgcca gaacactgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg
    16381 atggtattca tcgcggtaaa ttgcgtcgac atagccatcc ctaatataaa caacggcaag
    16441 atcagcatcc atatcgccat taccggtgat tgcaaagaga actgagcgat cattagccca
    16501 ataatcaccg tgatccccac taacgtatgg cgatagccca gacgacgtaa gacttgggta
    16561 accatcgatt ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata
    16621 aacgcctgat aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacaaccg
    16681 gtccccagac gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat
    16741 aaatctaatg aaattaatgg gtttggcgtg tgtcgcgcat ggagaatata gagaagcagt
    16801 aacccgatgc tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg
    16861 aatagctcta ttccgcttga gaagagaaca aggctgaggc caaacagcaa aaagccagtg
    16921 atatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg
    16981 cccgcaatac ctatggggat attgattaaa aatatccagt gccaggttgc ccaggtgacc
    17041 agcacgccgc caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa
    17101 ttcaatactg gaagaagttc attacgagga taagcgcgca gtaaggccag ccgagcaaca
    17161 ggcatcatca ttgcgccgcc tatcccctga ataacccgga agacaaccag ctgtggtagc
    17221 gaattagaaa gtgcgcaggc cagagaaccc aatgtgaaca gactcacggc aagggtaaaa
    17281 atgcgacgcg taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc
    17341 gtcagcgtat aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga
    17401 gcgattgcgg gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaagaaggcc
    17461 atcgccgcga tccacggcaa acccgccata ctgcgcttct ttttatcgct cattcaatgt
    17521 cctattatcg ggttgtcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc
    17581 cgcgtcgcca tcgctttgga caatcgcatc gacaatcgcc tggtgcagat ccagctttat
    17641 cactgtgtcg ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc
    17701 gaatgaggtc aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggccatatc
    17761 gacttcgatc cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa
    17821 cgtattgaga tgcgccttct gttccgcggt gccaaccgtt gcagccagta ggcaagcttg
    17881 cggctccaga cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc
    17941 tgtcatccac caggtaagca attcctgatc aagaaaattc cagtttgatt gtggcatgac
    18001 ccgggtacca attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc
    18061 ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccaggcaa
    18121 aatggtgccg ggttcatatt caccttttaa gatgcgttgc gccagcttct cagccagaac
    18181 atacgaaagg tttttctgtg cagctaactg ttgtgcgctt aaaggcatta ctaatcttcc
    18241 tttttctttt tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg
    18301 gcaaattgct tgttttatgg cacattaacg gggcttttgc t
//
LOCUS       DAZFJF010000093        18301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFJF010000093 DAZFJF010000000
VERSION     DAZFJF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.093"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..601)
                     /locus_tag="ACHKAU_003487"
     CDS             complement(<1..601)
                     /locus_tag="ACHKAU_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532483.1"
                     /translation="MDMIPSYESMYLTLRGIPTDSIGGEDIYFIHDPQGGWYPNRGNH
                     LYAIDALCVNIHDALANSIFDGLENYHKFLYMAPEFLSTAGLNSESVVSKETFVLFID
                     KFKGHTDVNKGLYLFDCCKIVSSIQECSKEVLQLQGEFYYTLNFEPLFFPNIEEEDGI
                     RYVTSPVVTKLFALLGFIYIRMYSLLDYITKLAIEIENLK"
     gene            933..1805
                     /locus_tag="ACHKAU_003488"
     CDS             933..1805
                     /locus_tag="ACHKAU_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5532484.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            2175..5021
                     /locus_tag="ACHKAU_003489"
     CDS             2175..5021
                     /locus_tag="ACHKAU_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO5532485.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQNVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFHK
                     LTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            5349..6167
                     /locus_tag="ACHKAU_003490"
     CDS             5349..6167
                     /locus_tag="ACHKAU_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5532486.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFAYQIRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            6259..6744
                     /locus_tag="ACHKAU_003491"
     CDS             6259..6744
                     /locus_tag="ACHKAU_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5532487.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRMENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            6760..7236
                     /locus_tag="ACHKAU_003492"
     CDS             6760..7236
                     /locus_tag="ACHKAU_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5532488.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            7299..7520
                     /locus_tag="ACHKAU_003493"
     CDS             7299..7520
                     /locus_tag="ACHKAU_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5532489.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            7594..8001
                     /locus_tag="ACHKAU_003494"
     CDS             7594..8001
                     /locus_tag="ACHKAU_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5532490.1"
                     /translation="MSDTLHGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCSFVYMAVYATRFSNQLPKASVHQLTHYF"
     gene            complement(8542..9789)
                     /locus_tag="ACHKAU_003495"
     CDS             complement(8542..9789)
                     /locus_tag="ACHKAU_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO5532491.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(9861..10775)
                     /locus_tag="ACHKAU_003496"
     CDS             complement(9861..10775)
                     /locus_tag="ACHKAU_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO5532492.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            10991..12424
                     /gene="cscA"
                     /locus_tag="ACHKAU_003497"
     CDS             10991..12424
                     /gene="cscA"
                     /locus_tag="ACHKAU_003497"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO5532493.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(12432..13427)
                     /locus_tag="ACHKAU_003498"
     CDS             complement(12432..13427)
                     /locus_tag="ACHKAU_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5532494.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            13670..13888
                     /locus_tag="ACHKAU_003499"
                     /pseudo
     CDS             13670..13888
                     /locus_tag="ACHKAU_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            13906..15234
                     /gene="dsdA"
                     /locus_tag="ACHKAU_003500"
     CDS             13906..15234
                     /gene="dsdA"
                     /locus_tag="ACHKAU_003500"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO5532495.1"
                     /translation="MENAKMNSLIVQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(15342..16880)
                     /gene="emrY"
                     /locus_tag="ACHKAU_003501"
     CDS             complement(15342..16880)
                     /gene="emrY"
                     /locus_tag="ACHKAU_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO5532496.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(16880..18043)
                     /gene="emrK"
                     /locus_tag="ACHKAU_003502"
     CDS             complement(16880..18043)
                     /gene="emrK"
                     /locus_tag="ACHKAU_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO5532497.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         4835 a         4601 c         4436 g         4429 t
ORIGIN      
        1 tttttaaatt ttcaatttct atagctagtt ttgttatgta gtcgagcaaa ctgtacatgc
       61 ggatataaat aaatccaagt aatgcaaaga gcttagtcac gactggagat gtaacatatc
      121 ttataccgtc ctcctcttca atgttaggga aaaacaaggg ttcaaaattc aacgtgtaat
      181 aaaactcccc ttgtagttgc aacacttcct tgctgcattc ctgtatgcta cttacaattt
      241 tacagcaatc aaaaaggtaa aggcctttat ttacgtcagt atgccctttg aatttatcaa
      301 taaataaaac aaatgtttct tttgaaacta cggattcaga gttcaaacct gctgttgata
      361 aaaactcagg ggccatataa aggaacttgt ggtagttttc taatccatca aaaatgctat
      421 tagctagagc atcatgaata ttaacgcata atgcatcgat cgcataaaga tgattccctc
      481 tgttcgggta ccatccacct tgaggatcat gtataaagta aatgtcctca cctccaattg
      541 agtcagttgg tatccctctt aaagtcaaat acatgctttc atatgaaggt atcatatcca
      601 tgtttttctt taattcctct tatgaatcga aaaagtgagg tttcgtatat aggttaattt
      661 aagcaagata atcatactct gaaagtaata atttttcata tccgggttta tcaaacgcag
      721 ggaattttat gggcttttct ttactcaacc taacgcccgc ttctggcaca gagcggactg
      781 tcagattaga ttcagacctg tactcattag ctgatacaaa tcagccacat atacctgcat
      841 tccaaataat ttcagacaga tattatcgtt gtgaatcccc cgccaccttt ccggtgcgcg
      901 gggttttgtc ttttttcacc gggaatacat gtatgaatcc gtctgatgcc attgaggcaa
      961 ttgaaaaacc gctctcctcc ctgccttact cactttcccg tcacatcctg gaacatctgc
     1021 gcaaactcac cagtcacgaa cccgtgattg gcattatggg taaaagcgga gccggtaaat
     1081 cctcactctg taatgcactg tttcaggggg aggtcacccc ggtcagtgat gttcacgccg
     1141 gcacccggga agtgcggcgc ttccgtctga gtggccacgg tcacagcatg gttatcactg
     1201 acctgcccgg ggtgggcgag agccgggaca gggatgcaga gtatgaagcc ctgtaccgtg
     1261 acattctgcc cgaactggac ctggtgctgt ggctgattaa agccgatgac cgcgccctgt
     1321 ctgtggatga gtatttctgg cgacacatcc tgcaccgggg acatcagcag gtgctgtttg
     1381 tggtgacgca ggccgacaaa acggagccct gccatgaatg ggatatggcc ggtattcagc
     1441 cttctcccgc acaggcacag aatattcgcg aaaaaacgga ggcggtattc cgtctgttcc
     1501 ggcccgtaca tccggttgtg gccgtatcgg cccgcaccgg ctgggaactg gatacgctgg
     1561 tcagtgcgct catgacagcg cttcccgacc atgccgccag tcccctgatg acccgcctgc
     1621 aggacgagct gcgcacggaa tctgtccggg gtcaggcccg tgaacagttt accggtgcgg
     1681 tggaccggat atttgacacg gcggagagcg tctgtattgc ctctgttgca cgcacggttc
     1741 tgcgggccgt ccgtgactcg gtggtctctg ttgcccgcgc ggtatggaac tggattttct
     1801 tctgaacact ctgccagggg aaatgcctga ttacattgcg ttaaccaact gaaaacatgc
     1861 cccccccctc atacccacct ctttctcttg cgtgactccc ctgctgttaa tagaataaaa
     1921 cgatcgataa aacaggtatc gatcgttact atcgatcgtt tatagtggtt aatggtctgc
     1981 gaaaaggcag ctgaatctct tcatcatgca gaacggaatt gcacacaacg gactgatact
     2041 tctctggcgg atgagagagg ggaaacattt atgtccggga aaaaccactt tatctgacca
     2101 tgctgttttg tacctgccgg tatccacttt tgtgggtacc ggctttttta ttcaccctct
     2161 gtaaggaaaa gctgatgaaa cgacatctga acaccagcta caggctggta tggaatcaca
     2221 ttacgggcac cctggtggtg gcctccgaac tggcccgctc acggggaaaa cgcgccggtg
     2281 tggcggttgc gctgtctctt gctgctgtca catcagtccc ggcactggct gctgacacgg
     2341 ttgtacaggc gggagaaacc gtgaacggcg gaacactgac aaatcatgac aaccagattg
     2401 tcttcggtac ggccaacgga atgaccatca gtaccggtct ggagtatggg ccggataacg
     2461 aggccaatac cggcggacaa tggatacaaa atggcggtat cgccaacaac actactgtca
     2521 ccggtggtgg tcttcagaga gtgaatgccg gaggaagcgt ttcagacacg gttatcagtg
     2581 ccggaggcgg acagagcctt caggggcagg cagtgaacac cactctgaac ggcggtgagc
     2641 agtgggtaca tgaaggcggg attgcaacgg ttaccgtcat taatgagaag ggctggcagg
     2701 ccgtcaaatc cggcgcaatg gcaaccgaca cggttgtgaa taccggcgcg gaagggggac
     2761 cggatgcaga aaatggtgat accgggcaga acgtctacgg agatgccgta cgcaccacca
     2821 tcaataaaaa tggtcgtcag attgtggctg ctgaaggaac ggcaaatacc actgtggttt
     2881 atgccggcgg cgaccagact gtacatggtc acgcactgga taccacgctg aatggggggt
     2941 accagtatgt gcacaacggc ggtacggcgt ctggcactgt tgtaaacagt gacggctggc
     3001 agattatcaa ggaaggtggt ctggcggatt tcaccaccgt taaccagaaa ggcaaactgc
     3061 aggtgaacgc cggtggtacc gccacacatg tcaccctgaa acagggtgga gcactggtca
     3121 ccagtactgc ggcaacggtt cttggcagca accgtctggg caatttcacg gtggaaaacg
     3181 gtaaggctga cggcgttgtt ctggagtccg gcggtcgcct ggatgtactg gaaggccatt
     3241 cagcgcagaa aacacgggtg gatgacggcg gtaccctggc agtgtctgcc ggtggtaagg
     3301 caacagatgt caccatgaca tccggtggtg ccctgatagc agacagtggt gccactgttg
     3361 aggggaccaa tgccagcggt aagttcagta ttgatggcat atccggtcag gccagcggcc
     3421 tgctactgga aaatggcggc agctttacgg ttaatgccgg gggacaggca ggcaacacca
     3481 ctgtcggaca tcgtggaaca ctgacgctgg ctgccggggg aagtctgagt ggcagaacac
     3541 agctcagtaa aggcgccagt atggtactga atggtgatgt ggtcagtacc ggcgatattg
     3601 ttaacgcagg agagattcac tttgataatc agacgacaca ggatgccgtg ctgagccgtg
     3661 ctgttgcaaa aggcgactcc ccggtaacgt tccataaact gaccaccacc aacctcactg
     3721 gtcagggcgg caccatcaat atgcgtgttc gccttgatgg cagtaatacc tcagaccagc
     3781 tggtgattaa tggtggtcag gcaaccggca aaacctggct tgcgttcaca aatgtcggaa
     3841 acagtaacct cggggtggca accaccggac agggtatccg ggttgtggat gcacagaatg
     3901 gtgccaccac agaagaaggt gcgtttgccc tgagccgccc gcttcaggcc ggcgccttta
     3961 actacaccct gaaccgtgac agcgatgaag actggtacct gcgcagtgaa aatgcttatc
     4021 gtgctgaagt ccccctgtat gcctccatgc tgacacaggc aatggactat gaccggattc
     4081 tggctggctc ccgcagccat cagaccggtg taaacggtga aaataacagc gtccgtctca
     4141 gcattcaggg cggtcatctc gggcacgata acaacggcgg tattgcccgt ggggccacgc
     4201 cggaaagcag cggcagctat ggcttcgtcc gtctggaggg tgacctgctc agaacagagg
     4261 ttgccggtat gtctctgacg acaggggtgt atggtgctgc aggccattct tccgttgatg
     4321 ttaaggatga tgacggctcc cgcgccggca cggtccggga tgatgccggc agtctgggcg
     4381 gatacctgaa tctggtacac acatcctccg gcctgtgggc tgacattgtg gcacagggaa
     4441 cccgccacag catgaaagca tcatcggaca ataacgactt ccgcgcccgg ggctggggct
     4501 ggctgggctc actggaaacc ggtctgccct tcagtatcac tgacaatctg atgctggagc
     4561 cacaactgca gtacacctgg cagggactct ccctggatga cggccaggat aacgccggtt
     4621 atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggcttccgt ctgggcagcc
     4681 acaacgatat gaactttggt aaaggcacct catcccgtga caccctgcgc gacagtgcca
     4741 aacacagtgt gcgtgaattg ccggtgaact ggtgggtaca gccttctgtt atccgcacct
     4801 tcagctcccg gggagacatg agcatgggta cagccgcagc cggcagtaac atgacgttct
     4861 caccgtcccg gaatggcacg tcactggacc tgcaggccgg actggaagcc cgtgtccggg
     4921 aaaatatcac cctgggcgtt caggccggtt atgcccacag tgtcagcggc agcagcgctg
     4981 aaggttataa cggtcaggcc acactgaacg tgactttctg ataatcaggt cacttccctt
     5041 caggccccgg tcattatgac cggggccttt tactttcagg gatcaacaga tatgccgggt
     5101 tgtgcatcac gtttattgcc cgaagaccct tttactgcca aatggcatct gaattgatca
     5161 cagtctggcc agttattacc cgatcccgat ggggatattg ggtgatctcc ttccatgaaa
     5221 atacgcagct caccagtgga gggcttctca gacaataaac gtcattcatt tcatctgtca
     5281 ggccgcgtct tctccgggag acgcggcttt ttcatttata ccgctaactc attcataagg
     5341 agcaaataat gcgattagca agtcgttttg gtcatgtaaa ccagatacgc cgtgaccgcc
     5401 cgctgacaca tgaagaactg atgcactatg tgcccagtat ttttggggaa gaccggcata
     5461 cctcccgcag tgaacgctat gcgtacattc ccaccatcac cgtactagaa agcctgcagc
     5521 gggagggctt tcagcccttc ttcgcctacc agatccgcgt gcgcgacccg ggccgccggg
     5581 gatacaccaa acacatgctg cgtctgcgcc gtgacgggga gataaacgga caacatgtcc
     5641 ctgaaatcat cctgctgaac tcccatgacg gtacttccag ctaccagatg ctgccgggtt
     5701 acttcaggtt cgtctgccag aacgggtgcg tctgtggcca gtctctgggg gaagtgcgtg
     5761 ttccacaccg gggaaatgtg gtggagaaag ttatcgaagg ggcttacgag gtggtgggcg
     5821 tgtttgaccg gatagaggag aagcgtgatg ccatgcagtc gctggtcctg ccgccaccgt
     5881 cacgccaggc gctggcacag gcggcactga cttaccgtta tggtgacgaa catcagcccg
     5941 tcaccaccgc cgacattctg acgccacgac gccgggagga ttacggtaag gacctgtgga
     6001 gtgcttatca gaccatccag gagaatatgc tgaaaggcgg gatttccggt cgcagtgcca
     6061 gaggaaaacg tatccatacc cgtaccattc acagcatcga caccgacatt aagctcaacc
     6121 gggcgttgtg ggtgatggca gaaacactgc tggagagcat gcgctgatgc cttttccctg
     6181 aaagcgcagt cctgactcac ttccgtccct gcctccggac attcttccat tcatttactt
     6241 tttataagga ataatctcat gacaaccgtt acgcataatt ccaccacacc ttctgtttcc
     6301 gtaaccgctg catcagggaa taacccacct cagctggttg ctactctcgt ccctgatgaa
     6361 cagcgcatca gcttctggcc gcagcatttt ggcctcattc cacagtgggt gaccctggag
     6421 ccccgtgtct tcggctggat ggaccgtctg tgcgaagact actgcggtgg tatctggaat
     6481 ctgtacaccc tgaacaacgg cggggcattt atggcacccg aaccggatga cgatgatgac
     6541 gaaacatggg tactgttcaa tgccatgaac ggtaaccgcg ctgaaatgag cccggaagcc
     6601 gccggtattg ccgcctgtct gatgacgtac agtcatcatg cctgtcgtat ggagaattat
     6661 gccatgacgg tccattatta ccggttgcgg gattacgccc tgcagcatcc ggaatgcagc
     6721 gccattatgc gcatcatcga ctgaatggag agaagcacaa tgcaacaact ttcctttctg
     6781 cccggagaga tgacgcccgg cgagcgcagc ctcattcaac gggccctgaa aaccctggac
     6841 cgccatcttc atgaacccgg cgtggccttc acctccaccc gtgcagcacg ggaatggctg
     6901 attctgaaca tggcgggact ggagcgtgaa gaattccggg tgctgtatct gaacaaccag
     6961 aatcagctga ttgccggtga aaccctcttc accggcacca tcaaccgcac ggaagtccat
     7021 ccccgggaag tgattaaacg cgccctgtac cacaatgccg ctgccgtggt actggcacac
     7081 aatcacccgt ccggtgaagt cacacccagt aaggcagacc ggcttatcac cgaacgtctg
     7141 gtacaggcac tgggcctggt ggatatccgg gtgccggacc atctgatagt cggtggcaac
     7201 caggttttct cctttgccga acatggtctg ctttaacccg tcacaaccac atcacacctg
     7261 ttttcacttt tatcttctgt cttcagaggt atcccattat gaaaattatc acccgtggtg
     7321 aagccatgcg tattcaccaa caacatccga catcccgtct ttttccgttc tgtaccggta
     7381 aataccgctg gcacggcagt gctgaagcgt ataccggtcg tgaagtgcag gatattcccg
     7441 gtgtgctggc cgtgtttgct gaacgccgta aggacagttt tggtccgtat gtccggctga
     7501 tgagcgtcac cctgaactga gtgggaattc tgatgagcag aattatcgcc actaccggac
     7561 cattcttagc cgattttctg taaggatttt atcgtgtcag acacactcca cgggacaaca
     7621 cttcccgacg acaatcacga ccgcccctgg tgggggctgc cctgcaccgt gacgccctgt
     7681 ttcggggcac gtctggtgca ggaaggtaac cggttgcatt accttgccga ccgcgccggt
     7741 atcagaggcc ggttcagcga tgcggatgca taccatctgg accaggcctt tccactgctg
     7801 atgaaacaac tggaactcat gctcaccagc ggtgaactga atccccgcca tcagcatacc
     7861 atcacgctgt atgcaaaagg gctgacctgt gaagctgata ctctcggcag ttgtagtttc
     7921 gtttatatgg ctgtttatgc tacccgattc tcaaatcaat taccgaaagc ttctgttcat
     7981 caactaactc attactttta atgatggggc cctttctggg gcccaagaaa ttaacaacgc
     8041 taacaaacaa cataaataac aataaattaa ataaaaaatt tcattcctgc aggggacacc
     8101 agataccctt caaacgaaat ctaccttcac cccgtaaaag ataggtttgg cagcacactt
     8161 gccttatatc tactcatttt tactgcaaca ggttgaaatc tcagcactgt cagaaagcgc
     8221 tgatgactaa acagccctgg gccgggcgat gtaaccatca cacagaatcc tgatagcgaa
     8281 atatggcgtg actcgatact tcactccgca atgcattcct tgatgaattc gcaggaccgt
     8341 gatacacggg acaggtcgct gaatgacgac aatgtcctgg aaatcagcga accgcgcatc
     8401 tgaagtacat ttgagcgact gtaccagaac atgaatgagg cgtttggatt aggtgattat
     8461 tagcagggct aagcacttta gtattattat tttccggttg agggataggg agatatcgac
     8521 aacaaccgga aaagtttacg tctatattgc tgaaggtaca ggcgtttcca taactatttg
     8581 ctcgcgtttt ttactcagga agaaaatgcc aaatagcaac atcaggcaga caatacccga
     8641 aattgcgaag aaaactgtct ggtagcctgc gtggtcaaag agtatcccag tcggcgttga
     8701 aagcagcaca atcccaagcg aactggcaat ttgaaaacca atcagaaaga tcgtcgacga
     8761 caggcgctta tcaaaatttg ccacgctgta tttgaagacg gatatgacac aaagtggaac
     8821 ctcaatagca tgtaacagct tcactaatga aataatccag gggttaacga acagcgcgca
     8881 ggaaaggata cgcaacgcca taatcacaac accgataagt aatgcatttt ttggccctac
     8941 ccgattcaca aagaaaggaa taatcgccat gcatagcgct tcgagtacca cctggaatga
     9001 gttgagataa ccatacaggc gcgttcctac atcgtgtgat tcgaataaac ctgcataaaa
     9061 gacaggaaaa agttgttgat caaaaatgtt atagaaagac cacgtcccca caataaatat
     9121 gacgaaaacc cagaagtttc gatccttgaa aactgcgata aaatcctctt tttttacccc
     9181 tcccgcatcc gccgctacgc actggtgatc cttatcttta aaacacatgt tgatcatcat
     9241 aaatacagcg ccaaatagcg agaccaacca gaagttgata tggggactga tactaaaaaa
     9301 tatgccggca aagaacgcgc caatagcata gccaaaagat ccccaggcgc gcgctgttcc
     9361 atattcgaaa tgaaaatttc gcgccatttt ttcggtgaag ctgtcaagca aaccgcatcc
     9421 cgccagatac cccaggccaa aaaagagcgc ccccagaatt agacctacag aaaaattgct
     9481 ttgcagtaac ggttcataaa cgtaaatcat aaacggtccg gtcaagacca ggatgaaact
     9541 catacaccag atgagcggtt tcttcagacc gagtttatcc tgaacgatgc cgtagaacat
     9601 cataaataga atgctggtaa actggttgac cgaataaagt gtacctaatt ccgtccctgt
     9661 caaccctaga tgtcctttca gccaaatagc gtataacgac caccacagcg accaggaaat
     9721 aaaaaagaga aatgagtaac tggatgcaaa acgatagtac gcatttctga atggaatatt
     9781 cagtgccata attacctacc tgtcgttaaa aaattcacgt cctatttaga gataagagcg
     9841 acttcgccgt ttacttctca ctattccagt tcttgtcgac atggcagcgc tgtcattgcc
     9901 cctttcgccg ttactgcaag cgctccgcaa cgttgagcga gatcgataat tcgtcgcatt
     9961 tctctctcat ctgtagataa tcccgtagag gacagacctg tgagtaaccc ggcaacgaac
    10021 gcatctcccg cccccgtgct atcgacacaa ttcacagaca ttccagcaaa atggtgaact
    10081 tgtcctcgat aacagaccac caccccttct gcacctttag tcaccaacag catggcgatc
    10141 tcatactctt ttgccagggc gcatatatcc tgatcgttct gtgtttttcc actgataagt
    10201 cgccattctt cttccgagag cttgacgaca tccgccagtt gtagcgcctg ccgcaaacac
    10261 aagcggagca aatgctcgtc ttgccataga tcttcacgaa tattaggatc gaagctgaca
    10321 aaacctccgg catgccggat cgccgtcatc gcagtaaatg cgctggtacg cgaaggctcg
    10381 gcagacaacg caattgaaca gagatgtaac cattcgccat gtcgccagca gggcaagtct
    10441 gtcgtctcta aaaaaagatc ggcactgggg cggaccataa acgtaaatga acgttcccct
    10501 tgatcgttca gatcgacaag caccgtggat gtccggtgcc attcatcttg cttcagatac
    10561 gtgatatcga ctccctcagt tagcagcgtt ctttgcatta acgccccaaa aggatcatca
    10621 ccgacccgac ctataaatcc acttattccg cctaatctgg cgattcccac cgcaacgtta
    10681 gctggcgcgc cgccaggaca aggcagtagg cgcccgtctg attctggcaa gagatctacg
    10741 accgcatccc ctaaaaccca tactttggct gacatttttt tcccttaaat tcatctgagt
    10801 tacgcatagt gataaacctc tttctcgcaa aatcgtcatg gatttactaa aacatgcata
    10861 ttcgatcaca aaacgtcata gttaacgtta acatttgtga tattcatcgc atttatgaaa
    10921 gtaagggact atatttttat aaaagttaac gttaacaatt caccaaattt gcttaaccag
    10981 gatgattaaa atgacgcaat ctcgattgca tgcggcgcaa aacgcactag caaaacttca
    11041 tgagcaccgg ggtaacactt tctatcccca ttttcacctc gcgccacctg ccggatggat
    11101 gaacgatcca aacggcctga tctggtttaa cgatcgttat cacgcgtttt atcaacatca
    11161 cccgatgagc gaacactggg gaccaatgca ctggggacat gccaccagcg acgatatgat
    11221 ccactggcag catgagccta ttgcgctagc gccaggagac gataatgaca aagacgggtg
    11281 tttttcaggt agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt
    11341 ctggctcgat ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag
    11401 tcgggatggt attcatttcg agaaacaggg tgtgatcctc actccaccag aaggaatcat
    11461 gcacttccgc gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc
    11521 gaaagatcca ggcaacacgg ggcagatcct gctttatcgc ggcagttcgt tgcgtgaatg
    11581 gaccttcgat cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc
    11641 ggactttttc agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc
    11701 cgagggatac agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc
    11761 aggacgactt tttgcacaat ccgggcattt tactgaactt gataacgggc atgactttta
    11821 tgcaccacaa agctttttag cgaaggatgg tcggcgtatt gttatcggct ggatggatat
    11881 gtgggaatcg ccaatgccct caaaacgtga aggatgggca ggctgcatga cgctggcgcg
    11941 cgagctatca gagagcaatg gcaaacttct acaacgcccg gtacacgaag ctgagtcgtt
    12001 acgccagcag catcaatctg tctctccccg cacaatcagc aataaatatg ttttgcagga
    12061 aaacgcgcaa gcagttgaga ttcagttgca gtgggcgctg aagaacagtg atgccgaaca
    12121 ttacggatta cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact
    12181 tgttttgtgg cggtattacc cacacgagaa tttagacggc taccgtagta ttcccctccc
    12241 gcagcgtgac acgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat
    12301 taacgacggg gaagcggtga tgagtagtcg aatctatccg cagccagaag aacgggaact
    12361 gtcgctttat gcctcccacg gagtggctgt gctgcaacat ggagcactct ggctactggg
    12421 ttaacataat atcaggtgga acaacggatc aacagcgggc aagggatccg cgtcactctt
    12481 cccccttcac gaccttcaat aatatgcaat gcagcttccc gcccgataat gtcatgtgga
    12541 agctgaattg tggtcagcgg cggtaaaaac agatgcccga cgccaaccag attatcaaag
    12601 cccattacgg cgacatcctg cgggattcgt acccccttcg ccagaagaac ctgataagcc
    12661 acaaaggctg cgcgatcgtt accacatatc agaacatcaa aatctggttt gcccgatttg
    12721 aagtggtcat tgagtaaact tgcgagatcg gtgtagtgat catcacctgt tgccatgtga
    12781 aattgtttca cctcagccag atctcgtcca gcatcacgcc aggcctgctc aaatccctgc
    12841 cgacgatacc ctgttgccaa cgcactttcc ggtagccaga agcataacgg ttgacgatag
    12901 cccgccgcga gcaaatgctg tgttgattca tattgtgcag tgtaatcatc agggatataa
    12961 ctgggtaacg ctgggtcatc cgccacacag ttcgccaata caatattttc accatacaga
    13021 gactcaggca gcgtgatatg tcgcagcccc attgtagtat agataatgcc atccggacgg
    13081 tgggcaagca gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa
    13141 aaactattcc agccgaactc gctggcggtt tgctcaatgg caagcagaat atcaacagag
    13201 aaaggagtgg tagccgtgtc ctgcgccagc acggcgagag tcgacggctt acgtccttga
    13261 gcgcgcatct tacgggcgga aagatcagga acataattca gggtctggat tgcctgcaat
    13321 acgcggtcac gcgttgcagg acgcacagat tctgcattat gcatcacccg ggagactgtc
    13381 atcatcgaca ctcccgccag gcgtgcgaca tcctttaatg aagccatacc caagccgttt
    13441 gccgtaaaac gggcactgta gcagaaacag acgtcactgg cgagatccaa cgatattctc
    13501 aacgccctat cacccgacac ggcaatacaa taaaaaataa caataattgc cggataattg
    13561 tactcaattc cgcctctgtt ctcgccaacg agtaaccata taggcaagta agtaaaacat
    13621 gccaactggc atcgccaacc tggagaagaa aatataaata tggatcattg tgctgccgct
    13681 gtatcccgac atcagcccgg aaattattgc gattgctatc ggttcaggtg caattggctg
    13741 cacgatcgtt acggactcgc ttttctggct agtgaagcaa tattgcggcg ctacgctcaa
    13801 tgaaacattt aaatactata cgacagcgac atttatcgct tcagtcgtcg ctctggcggg
    13861 cacattcctg ctgtcattta tcatctaagc gcaaagagac gtactatgga aaacgctaaa
    13921 atgaactcgc tcatcgtcca gtatccgttg gtaaaggatc tggttgctct taaagaaacc
    13981 acctggttta atcctggcac gacctcattg gctgaaggtt taccttatgt tggcctgacc
    14041 gaacaggatg ttcaggacgc ccatgcgcgc ttatcccgtt ttgcacccta tctggcaaaa
    14101 gcatttcctg aaactgctgc cactgggggg attattgaat cagaactggt tgccattcca
    14161 gctatgcaaa aacggctgga aaaagaatat cagcaaccga tcagcgggca actgttactg
    14221 aaaaaagata gccatttgcc catttccggc tccataaaag cacgcggcgg gatttatgaa
    14281 gtcctggcac acgcagaaaa actggctctg gaagcggggt tgctgacgct tgatgatgac
    14341 tacagcaaac tgctttctcc ggagtttaaa cagttcttta gccaatacag cattgctgtg
    14401 ggctcaaccg gaaatctggg gttatcaatc ggcattatga gcgcccgcat tggctttaag
    14461 atgacagttc atatgtctgc tgatgcccgg gcatggaaaa aagcgaaact gcgcagccat
    14521 ggcgttacgg tcgtggaata tgagcaagat tatggtgttg ccgtcgagga aggacgtaaa
    14581 gcagcgcagt ctgacccgaa ctgtttcttt attgatgacg aaaattcccg cacgttgttc
    14641 cttgggtatt ccgtcgctgg ccagcgtctt aaagcgcaat ttgcccagca aggtcgtatc
    14701 gtcgatgctg ataaccctct gtttgtctat ctgccgtgtg gtgttggcgg tggtcctggt
    14761 ggcgtcgcat tcggacttaa actggcgttt ggcgatcatg ttcactgctt ttttgccgaa
    14821 ccaacgcact ccccttgtat gttgttaggc gtccatacag gattacacga tcagatttct
    14881 gttcaggata ttggtatcga caaccttacc gcagcggatg gccttgcagt tggtcgcgca
    14941 tcaggctttg tcgggcgggc aatggagcgt ctgctggatg gcttctatac ccttagcgat
    15001 caaaccatgt atgacatgct tggctggctg gcgcaggaag aaggtattcg tcttgaacct
    15061 tcagcactgg cgggtatggc cggacctcag cgcgtgtgtg catcagtaag ttaccaacag
    15121 atgcacggtt tcagcgcaga acaattgcgt aatgccactc atctggtgtg ggcgacggga
    15181 ggtggaatgg tgccggaaga agagatgaat caatatctgg caaaaggccg ttaataacgt
    15241 ttcaacgcag catcgcaatc ctttccctgg gtgagcgatg ctgccgatgg cgcagactta
    15301 agatccccgc tcttacccgc tataaccccc tccttttatc atcacccaac gcctttcgct
    15361 gtaaacggcg gtttcgcaaa ccaaaccaaa accgtaagta agataaaagc aatagccgcc
    15421 atacggaaaa tctcatttgc agaaatagaa agtgactgct gggttatttc attattaatt
    15481 tcattaagaa ctcctgaaag cgaaccatag tatttatcca taatttgtga cgatgaatta
    15541 aacacggggt taaattgatc gatggttgct gtcaactgac tatggtgtaa cgattcgcgt
    15601 cgtccccaca gcgtcattgt caacgacgta ccaactgatc ctgacaaggt acgaaaaaaa
    15661 ttactcatac tcgaggcatt ggcaaattta ttatctggca agcctgaaaa cgaaatcgtt
    15721 gttaagggta aaaagaaaca ggcaacggcg aatccctgga aaaactgtgg caaaatgatg
    15781 cctgtaaaat caatcgttgg cataaatgtc acagaacgcc agtaatagca aaccgcatac
    15841 atcaaaaaac taaatgtcac taacaaccgc atgtctattt tgttgccata acgtcctatc
    15901 aaaggtgaaa ttaatagtgg catgatgccg atgggcgcat aagcaagtcc ggcccatatc
    15961 gcattatacc ccatcgtttc ctggagtaac tgcggcataa ggacgatcgc tccagagtaa
    16021 aataaatacg cgcatgtgat actcacaata ccaatggtga agttacggga cttaaacaaa
    16081 ctgagatcaa gaatcgggtt ctctgaggtc gactcccaaa tgactaaaga gatcagagaa
    16141 ataactgata ctactgttaa tattattatt gtactcgagt tgaaccaatc cagatcgcgc
    16201 cctttatcaa gcataatttg caagccacca acaccgagca ctaacagggt cagtcctggt
    16261 agattcattt tgaccggtga agtttcagtt tctcttcctt taagtaaggt taagcataat
    16321 gtcaggacga taatccccat agggacattg attaaaaata tccaacccca gctaaagtta
    16381 tcgcaaatat aaccgcccaa tatcggccca catatcggag cgataatcac ggtcattgac
    16441 cataatgcca gagcaaatgt tcttttttct ggtggataat tccttaataa taaactctgt
    16501 gacagtggaa ttaacggccc cgccattaat ccctgaacga ctctaaaaaa tatcagcaca
    16561 tcaagattgg tcgataaact acacattaag gaagacagcg aaaaaaaagt gactgaaagt
    16621 aaaaataacc ttaattcgcc tattctttgt gccagcctgc cggtaacagg gatcgcaatg
    16681 gcatttgcta cgccaaacga ggtgataacc caggtgcctt cgtctgttga tgctcccaga
    16741 aagccagata ttgtcggtat tgcgacgtta gaaatagtgg aatccaacat ttgcataaac
    16801 gtcgctaatg acaatgcaat agtaacgcac cataacgtcc caccggttaa tggtgccgga
    16861 gtcgatttag tgattgccat taaagttgtc cattatgcga aataatgttg ctaatttctt
    16921 tttctatcgg actggtatcg ataactaaag ccttactggt ataagccggc atggacgtca
    16981 cggttgaagc cagatcaggc atctcagcaa tgtcttcgtt cttcgtatca atagttgcag
    17041 tcatcgataa accaatgcgt aaggggtgtt ccatgagttc ttttggatca agagaaactt
    17101 caaccggtac acgctgaacg attttgatcc agttccctgt cgcattttgt gcaggtaata
    17161 aggagaacgc attgccggtt cccatattga tccctgtcac ccgaccatga aacacaacat
    17221 tttcaccata aagatcgctg ataatattga ctgattgacc aatccgtaca tccgtgagtt
    17281 gtgtttcttt aaagttggca ttaacccaca tttgacgtgc cggtacgaca gccattaacg
    17341 attgtccggg actcactgtt tcgccgacct gaacactccg ctgggcaata tagccagtaa
    17401 ccggactcct tatatccgta cgtttaagcg ccaaccaggc ttctttagtt gcatccgccg
    17461 cttccacgac ttgtggctga cggtttaatg gtgtgttcat tactaaagct ttattcgctt
    17521 tataagcctg gatagcggca ttcagtgccg ctttgctact tattaacgta tctttggtat
    17581 gctccagcgt ttcttttgaa ataaccccct gcttcgctaa cggtactcga cggttataat
    17641 cttctaaaga ttgttgatac tgaatacgtg ctgaagcgac ttcggcactg tattgtttat
    17701 cctgtaagta cagtttattc gtttgccgaa caatatttgc cagattattt ttagctttat
    17761 tgagtgcgat agtggcatca gttttatcca gtgaaactaa aatgtcacct tgtcgaacgt
    17821 agttcgtatc tttatgatta acgacagtga cactacctga gacttgtgca gaaattggat
    17881 ctgcattccc cgtgacatag gcgtcatctg tactaatcat gtcttctaat tccattgacc
    17941 aataggcata ggcacctgaa aacgcaataa ataaaactac tgccaataaa gaaaagtatt
    18001 ttcttctgtt agaatgtttt ttatttgaat taatctgttc cactattatc tctcatttct
    18061 catagatgaa atttatgaat tgaatgaaaa gagcattcat tcttcatatc aaacacagtg
    18121 catgtgttgc acattatccc aggaacgttg gaggcctaat ttaaatgagg tgcaaaaaaa
    18181 acaacagagg actaaaccgt ggcttttgca atacaattct tacgcctgta ggattagtga
    18241 gaagacttat agtgctaact tgaaactata aatcatcggt ataatccctg attttattgt
    18301 t
//
LOCUS       DAZFJF010000094        17785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFJF010000094 DAZFJF010000000
VERSION     DAZFJF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.094"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            150..866
                     /locus_tag="ACHKAU_003503"
     CDS             150..866
                     /locus_tag="ACHKAU_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5532498.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            896..1396
                     /locus_tag="ACHKAU_003504"
     CDS             896..1396
                     /locus_tag="ACHKAU_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO5532499.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1488..4172
                     /locus_tag="ACHKAU_003505"
     CDS             1488..4172
                     /locus_tag="ACHKAU_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO5532500.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4169..5243
                     /locus_tag="ACHKAU_003506"
                     /pseudo
     CDS             4169..5243
                     /locus_tag="ACHKAU_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(5331..6629)
                     /gene="exuT"
                     /locus_tag="ACHKAU_003507"
     CDS             complement(5331..6629)
                     /gene="exuT"
                     /locus_tag="ACHKAU_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO5532501.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7112..8524
                     /gene="uxaC"
                     /locus_tag="ACHKAU_003508"
     CDS             7112..8524
                     /gene="uxaC"
                     /locus_tag="ACHKAU_003508"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO5532502.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8539..10026
                     /gene="uxaA"
                     /locus_tag="ACHKAU_003509"
     CDS             8539..10026
                     /gene="uxaA"
                     /locus_tag="ACHKAU_003509"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO5532503.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10109..10660
                     /gene="ygjV"
                     /locus_tag="ACHKAU_003510"
     CDS             10109..10660
                     /gene="ygjV"
                     /locus_tag="ACHKAU_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO5532504.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10665..11909)
                     /gene="sstT"
                     /locus_tag="ACHKAU_003511"
     CDS             complement(10665..11909)
                     /gene="sstT"
                     /locus_tag="ACHKAU_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO5532505.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12308..13273)
                     /gene="alx"
                     /locus_tag="ACHKAU_003512"
     CDS             complement(12308..13273)
                     /gene="alx"
                     /locus_tag="ACHKAU_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO5532506.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13556..14542)
                     /gene="ygjR"
                     /locus_tag="ACHKAU_003513"
     CDS             complement(13556..14542)
                     /gene="ygjR"
                     /locus_tag="ACHKAU_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5532507.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14621..15300)
                     /gene="ygjQ"
                     /locus_tag="ACHKAU_003514"
                     /pseudo
     CDS             complement(14621..15300)
                     /gene="ygjQ"
                     /locus_tag="ACHKAU_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(15377..15880)
                     /gene="ygjP"
                     /locus_tag="ACHKAU_003515"
     CDS             complement(15377..15880)
                     /gene="ygjP"
                     /locus_tag="ACHKAU_003515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO5532508.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15965..17101
                     /gene="rlmG"
                     /locus_tag="ACHKAU_003516"
     CDS             15965..17101
                     /gene="rlmG"
                     /locus_tag="ACHKAU_003516"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO5532509.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17292..17375
                     /locus_tag="ACHKAU_003517"
                     /pseudo
     CDS             17292..17375
                     /locus_tag="ACHKAU_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17372..>17785
                     /gene="higA"
                     /locus_tag="ACHKAU_003518"
     CDS             17372..>17785
                     /gene="higA"
                     /locus_tag="ACHKAU_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
                     /protein_id="HFO5532510.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         4731 a         4378 c         4499 g         4177 t
ORIGIN      
        1 tcattgtgaa agctactaaa tttcagtaag ttaattccca gcctttaaat ttaatattaa
       61 ctaaaaatgc ttttttatat tgacttacaa taaatctaca tctctaatac accaactttt
      121 tggaaacaat attgtaagat caaatttata tggtggcgaa aattaatctc ttatcacggc
      181 ttacgccgct tttattcgtg ttcgctcctt ttttggcaca gtcaaatatg actgtttatc
      241 ctatggctgt ttccattaat agtcagggtg aaggcaacgt tcgcgtaata tctaaaagta
      301 atgaagttca gtacatcaag gcgacagtat tccgtatcga taatccatcg acgcctcagg
      361 aaaatgaagt tgagattaaa tccggtgatg caaatcatct ggttgttatg ccacctaaat
      421 tcgctttacc agccggtagt agcaaaaccg tacgttttgt tgcgatggaa ccagagcaaa
      481 aagagaaaaa ttatcgcgtt aaatttgaag cggttcccag tattgatgac gttgccacag
      541 ataaaaaaga tctctctatg cagttaacag ttaacttaat ttgggggatt gttgttagtg
      601 ttccacctca gcaacctatt gctaagttag aagtaaatgc tgcccaaaaa ttagttaatg
      661 cagggaatca acgcttaaag attttaacga tcgcttattg taaaaataat agcaaagaaa
      721 attgtaagat acagaccgta aataaaaaca tcttccctgg tcaggaaaga aatcttgaaa
      781 gcatatctgg ctacgacaaa atcgttgtca aatataataa ctggatcacc aaagataatg
      841 gcgagtttga actggcggtc cattaattat cattgtaata aacgaaggaa tagtaatgaa
      901 aaaggtattt gcaaaatctc ttctggtcgc agcgatgttt tctgttgcag gctccgcatt
      961 ggcagtacaa aaggatatta ctgtaacggc caacgttgat gccgctctgg atatgacaca
     1021 gaccgataac accgcgttgc cgaaagcagt agaaatgcaa tatctgccgg ggcagggtct
     1081 gcaatcttac cagttgatga ccaaaatctg gtctaacgac gttaccaaag atgtaaaaat
     1141 gcagttagtt tccccggcgc aactggttca aagcctggat gctagcaaga ttgttccgct
     1201 gacagtaacc tggggcggtg aagaaattaa agctgatgcg gcaaccactt ttaccgcaac
     1261 caaaatcttt gcttccgatg cgttaactaa cggttctctg gctaaaaacc tgatgtttgc
     1321 tcaaatcacc aaaggtgttc tggagacagg catctaccgt ggtgtagtaa gcatttatct
     1381 gtctcaggat atctaatcca gataacagaa ttaacacgta acaaagggag gggtagccct
     1441 cccttatttt aactgtttta gtatttgtta tggatttcag gattgccatg gataaaaaat
     1501 tactggctct tttgatcctg gcgagtctca gcccggcaga ggcgacatta accaaaattc
     1561 ccgcagggtt tgaggttatt gctcagggac agcaggagta tatcgaggtt tatttttcag
     1621 ggaaaagtct cggtaaatat tatgcaatgg ttaatcttga taccgtaaca tttcttgatc
     1681 cagcaagtct atataacaag ctggaactgg atgtagacga tcagaaaatc gcgcatatag
     1741 tgaaagaaaa attatcgcag ccgctagctc gccacggtga attggcttgc ggttatgtac
     1801 gtactgactc agggtgtggt tttctgaata ccgatacgct ggaaataatc tataatgatg
     1861 aagaaagttc ggcaacgttg tttattaatc cgcaatggaa ttcagctttc gatgcgaagt
     1921 cattatattt aaatccagac aaaaatactg ttaatgcttt tatacatcag caagacatca
     1981 atgttctggc acaggatgat taccaatcgt tgtctattca gggaaacggt gcgctgggaa
     2041 taacagaaaa tagttatatt ggtgcacact ggaatttcaa cggttatgat gcagatgatg
     2101 tcagtgacag taatgctgac atcagcgatc tctattatcg ttatgatttt ttacgtcgtt
     2161 attatgtgca ggcgggtcgc atggacaacc gcacactatt taatgcacaa ggcgggaact
     2221 ttacctttaa ctttctgcca ctcggtgcaa ttgatgggat gcgtatcggg tcgacactga
     2281 gctatttaaa ccaggcgcaa agccagcaag gaaccccagt aatggttctg ctttcgcgca
     2341 attctcgtgt tgacgcttat cgtaatgagc aacttttggg atcgttttat ctcaatagtg
     2401 gttcgcaatt tattgatacc agttcctttc cgccaggcag ctacagcgtg gcattaaaag
     2461 tctacgaaaa taaccaactc acccgcaccg agcttgtgcc gtttaccaaa accggcggtc
     2521 tgactgacgg aaatgcgcaa tggttcttac aggcaggtaa aactacatca caggtttctg
     2581 atgatgaaag ttcagcttat cagctaggag tacgcctgcc attacatccg caatatgagc
     2641 tctacgcagg gctggcgaat gccgatgatg tgagtgcttt cgagttaggt aataactgga
     2701 cggcagattt aggcggggtg gggaatcttg caatcagcgc cagcgtgttc cgtaacgatg
     2761 acggcggcaa aggtgatatg caacaggcca actggagtaa tccgggatgg ccgacgttgg
     2821 gcttttatcg gaccaactct gacggcgatg cttgtacaac cgacagcaga gagagctata
     2881 acgccttaag ctgttatgaa agtatttccg cgacggtttc acagaatttt gtcggctgga
     2941 atatgatgct gggttatacc cgcacacaaa ataacactga tgatagtttg cgttgggata
     3001 aacagcagag ctttgaaaat aactatcttc gccagacaac agcgcaaagt atctctgaaa
     3061 ctgtacaact tagcgcttcc cgcgcttttg tgatgcgtga ctggattttg agtacgtcag
     3121 tcggtgtttt ccatcgtaat gacaacggtg gcgataacga cgacaacggc ttgtacttat
     3181 cgttttcgtt atctgacacg ccaacgatgg atagcaataa caacagccat tcaacaaatg
     3241 tgtctacgga ttatcgttat agcgaacagg atggcgatca aacgtcatgg cagttatcgc
     3301 atacctttta taacgattca ttcagccata aagaacttgg cgtaaccgtc ggaggcctga
     3361 acaccgatac cataaacagc gcggttaacg ggcgttggga tgggcaatac ggaaatgtct
     3421 acgctaccgt atctgatagt tatgaccgta agaatcatga tcatctttcg gcctttacgg
     3481 gcacttatag ctctacgctg gctgtcagtc gctatggcgt taatttggga gccagtggta
     3541 cagacggttt gctgggtgcg gtactggtgg atgtgaaagg cttctctgaa caggatgaag
     3601 agagtcagga tctgcaactc gaagcgcggg tggcaggcag ccgaactttg cagcttggtc
     3661 aaagtgacag tgtgttgttc ccttatcctg gatttcagtc tggttttgtt gaggttaacg
     3721 acagtagcca gggcaatcag caggggacaa caaacatcat taacggtgca gggaatcgtg
     3781 aattaatgtt gttgcctggc aagctgcgct atcgcgaagt gtctgccagc tttaactaca
     3841 actatatcgg tcgtttatta ctgccggcag cggtgaaaaa attcccgatt gtggggctga
     3901 acagcgccat gttactggta gctgaagatg gcggatttac acttgaaatt aacggcagcg
     3961 aaaaagagct ctatctgctt tccgggcagc aattccttaa gtgtccactg agtgttgtaa
     4021 agaaacgcgc cagcattcgt tacagcggag atgttacttg tagtgtggtg acttattcac
     4081 aattaccgga gtcgattcag gttcaggcac agttgaaaca gcctaaatta cgtggaaacg
     4141 ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttgattgcgg cgatattggg
     4201 cttgtttggt tcaggcagct cctgacgtga ccagtgaaat tacgtatgat ttggcatctg
     4261 gtagagcgga ttattacttc tggaaggatg aagcgtcggc aggaaataat ggatatatgt
     4321 ggtatgaatg ttcgtatcct gacctccaac aaacctgtac agctaatgga aatatatcga
     4381 cagtacaaat ctatttaact gaacaacgca gtgggatgcg ttggccggta aaactcaaag
     4441 gatttaaaac agccattgta agtagcgatg aagcgccgcc aggatgcaag gggggcaaag
     4501 ggcttcagac gaatcttaag gattctaata gatcttcatg tacagaagat ggtcaacatt
     4561 attatatata cgatacaaag tttcttacgc tctaccttga gcagacagag atgaagaatt
     4621 tgccgattgg tggcgtctgg aaggggaaag ttaaattaca ttcgaacagc ccggcccagg
     4681 actatttcgc aaatattacc ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc
     4741 cggagttcgc ccacgccacg ccaagggtgc agttagactt gcatccaaca ggaagcgtta
     4801 acggcagcaa ctacgcgcaa gatctgacca tgttggacat gtgcctgtac gatggtttta
     4861 acggtaatgc catcagttat gaaatcatgc tcaaagatga agggcgacca gctgcagggc
     4921 gcagagacgg ttacttctct atctatcgtc agggagggac caccaccgac gagggagaac
     4981 gcattgatta ccgggtcaaa atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca
     5041 ataatgaaaa tatggtctgg aacagcatta acctgaaacg tgtgcgtccg gtggtactgc
     5101 ccggtattcg ctatgccgtg atgtgtgtgc cgacaccctt gactctggca gtcgataaat
     5161 tcagcgtgat ggataaacag gccgggtact acatgggcaa attgtcagta atctttacgc
     5221 cttccttgcc aaccatcaat taaccctgca aaccggacaa acgctttttt ctatataaac
     5281 gacgaagccg tctgataaag cggcttcgtc attacaactt aagttaaacg ttaatgttgc
     5341 ggtgcgggat cgttatgtgt ttcctgcgcc acctcaatcg ccggtttgtt ctgcaacacg
     5401 gtccagataa ccagcgcacc taacaggtcg aacactgcca gaactgcgaa cagcgggctg
     5461 aagccgatgg tgtcagccag tgcaccgaca accagcgcaa acagcgtact tgccagccat
     5521 gcggacatcc cggttaaacc gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa
     5581 gagagcgtaa tcagcgcgcc agacagtgcc tggtgggcaa aaccaccgat acacagcagc
     5641 ataattgcga catacgggtt ggtgaacaga ccgatcatac ccgggccaat catcagcaca
     5701 gcacccagcg ttacgaccat cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg
     5761 aacagcggcg gcaggtaacc accgaggata caaccgaggt cagcaaacag catcggcatc
     5821 caggcgaaca tcgcgatctc tttcaggtta aagccgtaaa ctttaaacat gaacagcggg
     5881 atccacgcgt taaaagtacc ccaggccggt tctgccagga aacgcggcag cgcgataccc
     5941 cagaactgac ggttacgcag gatctgacca acggacattt tcttcgccgt gctcacctgg
     6001 tgctgggctt cctgaccatt aataatatag tcgcgttctt catcggtcag atgcttctgg
     6061 tcgcgcggat gtttatagaa aatcagccat gccatcgccc agataaagct caatgcaccg
     6121 gagatgataa atgccatctg ccagctgtgc attacgattg cccataccac cagcggcggc
     6181 gcaatcatcg caccaatcga agaacctacg ttaaagtaac ctaccgcgat ggaacgctct
     6241 ttcgccggga accattcgga gctggctttc agacccgccg ggatcatcgc tgcttccgcg
     6301 gcaccgactg caccacgagc aatcgccagg ccaccccagc taccagccag cgcggttgca
     6361 ccacagaaca cggcccacag cacagcaaac attgcataac cgattttcgt acccagcaca
     6421 tccagtacat aacctgctac cggttgcatg accgtataag cagcagaata ggctgcgatg
     6481 atataggaat actgttgggt ggagatgttt aactcttcca tcagagttgg cgcagctgcc
     6541 gccacagtgt tacgcgtcag gtaaccaagc acggtgccga gcgtcaccag tgcgatcata
     6601 taccaacgta accctttaat tttacgcatg taaaacctca tcgttttgtt atatccgcag
     6661 gaagcgcggc catcatcccg ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa
     6721 aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt gctaattcgg cttccgtacc
     6781 ggttaacccg attcatctaa caaatgaatg aatcggcaat ttgcattccg tcgacttatg
     6841 atttgcgacg gcagaaagat aacttgtcat acaactttaa aaggtgagag ccatcacaaa
     6901 tgtgcgaata tttgtaggga cattacctga cgacagcgag gccagtactg gcgcggcctg
     6961 cagagagatt taccactttg agagtaattt ttttaactac gtttattgat ctaactcacg
     7021 aaaatatctt cggactctgg aaattggtgt gataactttg tcagcatcgc accataagca
     7081 agctagctca ctcgttgaga ggaagacgaa aatgactccg tttatgactg aagatttcct
     7141 gttagatacc gaatttgccc gccgtctgta tcacgactac gcaaaagacc agccgatttt
     7201 cgattaccat tgccatttgc cgccgcagca gattgcggaa gactatcgtt ttaaaaacct
     7261 gtatgacatc tggctgaaag gcgatcacta caaatggcgc gctatgcgta ccaacggtgt
     7321 ggccgagcgt ctgtgtaccg gtgatgcgtc tgaccgtgaa aaatttgacg cctgggcggc
     7381 gactgttccg cacactatcg gcaacccgtt ataccactgg acgcacctcg aactgcgtcg
     7441 tccgtttggt atcactggca aattgctttc tccgtcaact gccgatgaaa tctggaacga
     7501 atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc ggcatcatgc agcagatgaa
     7561 cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct ctggagcatc acgcagagat
     7621 cgccaaagac ggctctttca ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt
     7681 caacatcgaa caggcgacct ttaacgacta catggcgaag ctgggcgaag tttccgatac
     7741 cgacattcgc cgctttgctg acctgcaaac tgccttgacc aaacgtctgg atcacttcgc
     7801 cgctcacggc tgtaaagtgt ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa
     7861 cgaagcggaa ctggacagca ttctggcgcg ccgtctggct ggcgaaaccc tgagcgagca
     7921 cgaagtggca cagttcaaaa ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg
     7981 cggctgggta cagcagtacc acattggcgc gctgcgtaat aacaacctgc gtcagttcaa
     8041 actgctgggg ccggatgtag gctttgactc catcaacgac cgtccgatgg cagaagagtt
     8101 gtctaagctg ctgagcaagc agaacgaaga aaacctgctg ccgaaaacca ttctgtactg
     8161 cctgaacccg cgcgataacg aagtgctggg caccatgatc ggtaacttcc agggcgaagg
     8221 tatgccgggc aaaatgcagt tcggttccgg ctggtggttt aacgatcaga aagacggtat
     8281 ggaacgtcag atgacccaac tggcgcagct cggtttgctg agccgctttg tcggtatgct
     8341 gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg
     8401 ccagatgatc ggtcgctggg tggaagcggg cgaagcaccg gcggacatca acctgctggg
     8461 cgagatggtg aaaaatattt gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa
     8521 ctaaggtctg gggttgatat gcaatacatc aagatccatg cgctggataa cgtcgcggtc
     8581 gctttagcgg atttggctga aggcacagaa gtcagtgtcg ataaccagac tgttacgctg
     8641 cgccaggatg ttgctcgtgg acataaattt gcgttaacgg atatcgcaaa aggggccaat
     8701 gtcatcaaat atggcctgcc gattggttat gcattggcgg atattgcggc gggggaacac
     8761 gtccacgccc acaatacgcg cacgaatctg agcgatctgg atcagtatcg ctatcaacct
     8821 gattttcagg atctgcctgc gcaagcggca gatcgtgaag tgcagatcta tcgtcgcgct
     8881 aacggcgatg tcggggtgcg taacgagctg tggatcctgc caaccgtggg ctgtgtcaac
     8941 ggcatcgcgc ggcaaatcca gaaccgtttc ctgaaagaga ccaacaacgc cgaaggtacc
     9001 gacggcgtgt tcctcttcag ccacacctac ggctgctcac aactgggcga cgatcacatc
     9061 aacacccgca ccatgctgca aaacatggtg cgccacccga acgcgggcgc agtgctggtg
     9121 attggtctgg gctgtgaaaa caaccaggtt gccgcattcc gtgaaacgtt gggcgatatc
     9181 gatcctgaac gcgttcattt catgatctgc caacagcagg atgatgagat cgaagccggg
     9241 atcgagcatt tgcatcagct gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa
     9301 ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg acggtctttc tggtattact
     9361 gctaacccga tgctggggcg tttctctgac tacgtgattg ctaacggcgg tactaccgta
     9421 ctgaccgaag tgccggagat gtttggcgca gagcagttgc tgatggacca ttgccgcgac
     9481 gaagcaacgt ttgaaaaact ggtgaccatg gtcaacgact tcaaacagta ctttattgcc
     9541 catgaccagc cgatctatga aaacccatcg ccggggaaca aagcgggcgg tatcaccacg
     9601 ctggaagata aatcccttgg ctgtacccag aaagcgggtt ccagcgtcgt ggttgacgtg
     9661 ctgcgttacg gcgagcgtct gaaaacgccg gggctgaact tgttaagtgc gccgggtaac
     9721 gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc atatggtgct gttcagtact
     9781 ggtcgtggca cgccatacgg cggctttgta ccgacggtga aaatcgctac caacagtgaa
     9841 ctggcggcga agaaaaaaca ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa
     9901 gcgatgccgc agttgctgga agaatttatc gacaccatcg ttgagtttgc caacggtaaa
     9961 caaacctgca acgagcgtaa cgacttccgc gaactggcga tcttcaaaag cggcgtaacg
    10021 ctataaagtc gaaatgagtt gttaataaaa aacggcgttt cataatgtga agcgccgttt
    10081 gcttttccat acctgataag gatttatcat gaccgcgtat tggctggccc agggcgtggg
    10141 tgtcatcgcc tttctgattg gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa
    10201 aaagcagctt tcggtctata gcgccgttat tggcgtacat ttttttcttc tgggcaccta
    10261 tcccgctggt gccagtgcca tccttaatgc cattcgtaca ttgattacct tacgcacgcg
    10321 cagcttatgg gtaatggcga tttttattgt gctgactggc ggaattggcc tcgcgaagtt
    10381 ccatcatcct gtcgaactat tgccggttat cggtacgatt gtcagtacct gggcgctgtt
    10441 ccgctgtaaa gggctcacca tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat
    10501 tcacaacttc tgggcggggt cgataggcgg cacaatgatt gagggtagtt ttctgcttat
    10561 gaatggcttg aatatcattc gtttctggcg gatgcagaaa aggggaattg atccgtttaa
    10621 agtagagaaa acccctcccg ccgtagacga aaggggttaa acaattaatt acgcagggcg
    10681 ctatttgcca gacgatcgtc ttccgcctgg caagctgccg cagtgaacag cacgtcagtt
    10741 gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc cgatgataaa gccgacggca
    10801 accacctgca tggcgatatc gttcgaaata ccgaacatat tacaggccag cgggatcagc
    10861 agcagagaac cccccgccac gccggatgcg ccacaggcac acagagaagc caccacgctc
    10921 aacagcagcg ccgtgggcag atcgaccggg atacccagcg tattaaccgc agccagcgtc
    10981 aacacggtaa tggtgattgc tgcgcccgcc atattgatgg tggcacccag cggaatagaa
    11041 acggaatagg tatcgcgatc cagattcagc ttttcacaca gtgccatatt caccggaatg
    11101 ttcgccgcag agctgcgggt gaagaaggca tacacgccgc tttcgcgcag gcacagcagc
    11161 accagcggga acgggttacg acgaattttc caccacacca gcaatgggtt aaccaccagc
    11221 gccaccagta acatacagcc aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg
    11281 gagaaaccgg tggttgccag ggtagaagaa accagaccaa aaataccaat cggtgcgaag
    11341 cgaatgacca gtttcaccat aaaggtgacg gcattcgaca tatcgttgac caggtttttg
    11401 gtggtctcgt taccgtgacg cagtgcgaaa ccgaggccaa ttgcccacac caggatcccg
    11461 atgtagttac ctttcagcag cgcgtcgatg gggttggaaa ccatgctcat taccagcccg
    11521 cgcatcactt ctacaatgcc tgacggtggc gaaatatcac ccgcgctact ggataagtgc
    11581 agggtagaag ggaaggcgaa gctgaaaacg actgcggcca gagcagcaga gaaggtaccc
    11641 agcagataga ggaacaaaat agggcggata ttggttttct gcccgtgctg gtggttagca
    11701 atagatgcca tcaccagcat caacaccagg atgggggcaa cggctttcag tgcgccgacg
    11761 aacaaagtac ctaacagacc aacagcttcc gccgcgggtt ttgagatcca tgccagaaga
    11821 atccccagaa caaggccgac caggatttgt tttaccaggc tgccatgagc cagacgccgg
    11881 aatagccccg gtgaacgttg cgtagtcatt tttcgatcct ttcattgtgt tgtcgcacat
    11941 ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt
    12001 ataaggaaag ttacagcttc gggaagcgaa aaatgctgga ttttaaagag aggtcatatt
    12061 ttgcaactta atggttacat ttatttgaca taaaaataaa gccaacgtgc ggcttccaga
    12121 ctgatgacaa catcaaaaaa ggactcggtc agtcccctcg ccccgtttgg ggagagggtt
    12181 agggtgaggg gaacaggcca gcatcggtgc gaaaaattac cctcaccccg accctctccc
    12241 tggaagggag agggagaaaa cggtgctcat gttaatgaca gggtacacgg cttaggcaga
    12301 ttaaaaatta tccaccccgc tgcttatcat gccgataatt cacccaggcg ttgataataa
    12361 acgtcatcac cagaatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga
    12421 agtcgacaat cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga
    12481 gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga
    12541 ttgcaaacag gtttgaggtc agcacaataa acggatcagt ggtcacggcg aagatagccg
    12601 gaatgctatc cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca
    12661 gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt
    12721 cggtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt
    12781 catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat
    12841 acaggatcca gtcgaactgt gaaatcagcc agctgcctgt gaagatcatg atggtacgca
    12901 gaacaatcgc cccgagcacg ccatacacca gcacgcggcg ttgtaatgcc gccggaacag
    12961 agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa
    13021 tcagatagcc cgtgagaaag gccagtgcct gtggatcggc aacggcgcga ccttcagttt
    13081 gcaccagata ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc cagaccagcg
    13141 accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca
    13201 ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc
    13261 cgacagtatt catagaagtt ccttacacat aaaaaaacgg ccaacgtcgg aagacgtcag
    13321 ccgctgcttt ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg
    13381 aatacgtctg cgtattcctt tcgggttgcc cggtgaccgg atgtggtttt tcacacatcg
    13441 taatgacgat cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac
    13501 cattcagcca gtcaatggtc tgttacatac cttttacact gttttacttt ggcaattata
    13561 gttttacgct atctgccgga aaaatcaccc cagtctggcg gcggatctcg gtcagcagtt
    13621 tggcggtgat gcgactgacc gccagccccg gatgatccac cagatgctca tccaccaggg
    13681 tagcgaacag ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct
    13741 gcatttggct gccacgcggc acgaagcaca ctttctggca ttcagacagt ttttcaatca
    13801 ccagcgatcc tgcttcgccc tgaatctcgc tcgccaggac agaatcactg acttttgagt
    13861 gctgcaaggt gacgctgaaa tcaccgtaat ccatcaccac cacgccgtgg gcatctacac
    13921 cgcttgccag caaactggcg gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg
    13981 ccgacgccag gcagtaaaag ccgatatcca taattgaacc gttagagaat gacggattaa
    14041 aggtgttggg attttcaccg tccaggtaac gttgatagcg cgaggaatac tggcaatagt
    14101 tgaaaaagac tttacgcaat ttgccgactt tcggcagcgc ctggcgcagc aaatgaaagt
    14161 tcggcaggca ggcggtttta aatgcctcaa acagcaccac ctgattttcc cgcgcacagg
    14221 caatggcggc atccacttcc gccagattcg acgccagtgg tttctcgcaa atcacatgaa
    14281 ttttatggct aaggaaaagt tgtgtctggg aaaagtgcag ggcattcggg ctggcaatat
    14341 acaccgcgtc aatggcatcg ctttccgcca tcgcttccag cgaggtaaac agatgctcga
    14401 cagaaaaatc attggcgaag tgctgagcct gctcaaggct gcgggaatat acggcggtta
    14461 acttgtattt accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac
    14521 caatcacagc gaaacgtatc ataacgcgtg gatactccat aaagggattc tgtcgtcaat
    14581 gtagcacgcc tttttcatct tcgacgtgcg cagatacgca ttattctttt gccggacagc
    14641 catgctcact aaacggccca atcatgaccg atggcccgag atatttcggc tggcgatgga
    14701 ggattttggc gtcaatcacg gcgctgacac gagcgagttt ctcccgcagt tgagtataaa
    14761 aaccgtgctt catattgagg tcaggcgcgt taaaaccgat agcgtcgatg ccgtactgtt
    14821 ttgccagcca gatggcgcgt tgattatgaa attcttgcga gataatggtg atatggtttt
    14881 caccaaaaac ctttttggct cgcactaccg aatccagcgt tgagaatccg gcatagtcgc
    14941 agaagatcac tttcgccggt acgcctttag cgatcaagcc tgcatacctg aggcttcatc
    15001 gtaattttta cgtccgttat cgccacttac cagtagccat ttcacttttc cggcgtggta
    15061 caacgctgct gcggtatcaa tacgtcgggt aaagtagcga ttacccggcc tggcccccag
    15121 caataagccc acgttacgcg ccggaactgc gttgacatcg ctccaggtca gctgcttgct
    15181 ggcgttgacc ataacgcgat cggcaataaa aatggtcgct cctgctataa gcaacaacaa
    15241 acagccaatc ttaagagtcc ggcgagaaaa acaaatacgg agaagaaggc ggggctgcat
    15301 ggtcgttatc cttaacgaaa ggggggaggt cgattttagg cgctaaagaa agaaaagata
    15361 acgcctgaca tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc
    15421 gggtgtcgaa ctcaagctgg tggtactgcg gctccatatg gcagcacaac tggtaaaacg
    15481 ctttgttatg ctctttctcc ttcaggtgcg ccagttcgtg caccacgatc atacgtaaaa
    15541 acggttccgg cgcgttgcga aacacagtag cgacgcggat ctctgctttt gctttcagct
    15601 tgccgccctg cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga
    15661 tcttattgtc atacatcact ttattgatcg gcggggcatt gcgcagaaac tgatttttta
    15721 gatccggagt atattgccag agggctttat cggtggcgta gtcgtgtgtt cccggatagc
    15781 gttttgccag cacatcgccc agacgctgtt cgttaatcag cgttcgcacc tgggaaagta
    15841 gctgctcggg ataaccctga agataagtaa gattgctcat aaccgcccac gaaaaagaga
    15901 aaaagggtat actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg
    15961 gccgatgagc cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac
    16021 ggatgacgtt aacccgctac aggcgtggga agcggcggat gaatatttgc tgcaacagtt
    16081 ggacgacaca gaaatccgcg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag
    16141 ctgcgcgctg gcggaacata agccgtacag cattagcgac tcatacatca gtgaactggc
    16201 gacgcgcgag aatttacgcc tcaacgggat tgatgagtcg agcgtgaagt ttctcgacag
    16261 caccgccgac tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc
    16321 attgctggaa caacaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat
    16381 tgccggtgcc aaagcccgtg acattcacac ttccacgctg gaactgttcg aaaaagtgct
    16441 cggcccgacc accaccacgc tggcatggaa aaaagcgcgc ctgattaatt gcacgttcaa
    16501 tgaaccgccg ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg
    16561 gactatccat aaccatgcga atgtcttctc ccgcaccggg cttgatatcg gcgcgcgctt
    16621 ctttatgcaa catctgccag agaatctcga aggggagatt gtcgatctcg gttgcggtaa
    16681 tggcgttatt ggtctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga
    16741 tgaatcgccg atggcggttg cttccagccg tttgaacgtt gaaaccaaca tgccagaggc
    16801 gttggatcgc agcgagttta tgatcaacaa cgcgctctcc ggcgtggagc ctttccgctt
    16861 taatgctgtg ctctgcaacc cgccgtttca ccaacaacat gcgctgaccg ataacgtcgc
    16921 ctgggagatg ttccaccatg cccgccgctg cctgaaaatc aacggcgagc tgtatattgt
    16981 tgccaaccgt cacctggatt acttccataa actgaagaag attttcggca actgcaccac
    17041 catcgccacg aataataaat ttgtggtgct gaaagcagtg aagctggggc gtcgtcggta
    17101 agattgggag ttacctgccg gatgcggcgt taacgcctta tccggcctac gttcagcacg
    17161 ttactcccag gccggataag acgcggcaag cgtcgcatcc ggcaatgtct gcccagttct
    17221 ggaatcgcgc ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta
    17281 gcgttgttca acgtttatgc tacatacgtg aagttatgac gcataaagaa tacgatttct
    17341 ttaccgctgt tcatcgtact aaggggaaaa aatgattgcg attgccgaca tcttgcaagc
    17401 aggagaaaag ctaactgctg tggcaccttt tctggcgggt attcagaacg aggaacaata
    17461 cacccaggcg ctggaactgg tagatcatct gctgctcaac gatcctgaaa accccttgct
    17521 ggatctggtg tgtgcaaaaa taaccgcgtg ggaagaatca gcgcccgaat ttgcggaatt
    17581 taatgccatg gctcaagcca tgcctggcgg tatagccgtg attcgtaccc ttatggatca
    17641 atatggttta accctttccg atctgccgga aattggcagt aaatctatgg tgtcacgcgt
    17701 tttgagcggg aagaggaaat taacgctgga acacgctaaa aaattggcaa cgcgattcgg
    17761 catttctccc gccttgttta ttgat
//
LOCUS       DAZFJF010000095        17645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFJF010000095 DAZFJF010000000
VERSION     DAZFJF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.095"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..331)
                     /locus_tag="ACHKAU_003519"
     CDS             complement(<1..331)
                     /locus_tag="ACHKAU_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532511.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKL"
     gene            complement(370..1605)
                     /locus_tag="ACHKAU_003520"
     CDS             complement(370..1605)
                     /locus_tag="ACHKAU_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO5532512.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARARALRDEYLSFLANGVDPQIHNNDKAK
                     ALKSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPK
                     LLKQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNM
                     PALPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNI
                     PADFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIR
                     MGFGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQS
                     LMQWWSDYVQAQRSNALVA"
     gene            complement(1904..2812)
                     /locus_tag="ACHKAU_003521"
     CDS             complement(1904..2812)
                     /locus_tag="ACHKAU_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFO5532513.1"
                     /translation="MARTLVNVSATIFALMMIVRALFTYIYPGKLPFSIAIIDWLIVI
                     AGSGAAISSIFCFIKKRYPDTAEFLPMFSTVCYVIILIGYAILRYTPAYQTSLSIMVT
                     GMLVGMGWWIQCITSAANTRRSHTLNMIINTRTSPEYQKQLRNSTKFYRGMRYVPQEL
                     SEWRCNPDKEEYKNMKVPDEYRDAINGLLYILNYFEFLAQGIKFKDLDDELLRECFSS
                     FLRGIERRGFHMILESQKQDPAAFEGIIYLSKKWNGTSFVETHRSNPNTVELGVPYPS
                     NDMVEKMVQGQPLIDSDAGPELQVAT"
     gene            complement(3295..4470)
                     /locus_tag="ACHKAU_003522"
     CDS             complement(3295..4470)
                     /locus_tag="ACHKAU_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO5532514.1"
                     /translation="MGKKEQGLLSLPRGVTIRKHKTASTLVITFTYKGVLCREPLSRL
                     EANTRGIKYAERLLGEIQNQIASGTFEYAKYFPNSKKLELFGVVKKTKNIKSYLDEYL
                     KICINRNLSPSTIGGYEKCLSALSELHKLHVTELTPAILKNWIASRKTKLKTIRNNLS
                     FLRSAIDEAVTDGLLTINPVTLVSASRYHVIDSSPSADDYEVDPFTPAETLAIYQSCR
                     YREWENLFRFAFNTGLRSSELCALRWADLDTIANTAHVQAASVVGVLKGTKTKAGTRK
                     VELNSEALAALQAQKQYTFMKSEFIFSDPKTGEPWTNADAIRKKAWVPTLKKAGVRYR
                     NPYQTRHTFATRHISQGVNLFWLAGQMGHKGPEMLFRNYGKYLAEYDGKTSINVVSS"
     gene            complement(4425..4634)
                     /locus_tag="ACHKAU_003523"
     CDS             complement(4425..4634)
                     /locus_tag="ACHKAU_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5532515.1"
                     /translation="MGLDCVPISTYCRNAGETVDAVNKRIQRGIWKEGVHVLKVDGVK
                     ERWVDLIEVSKWARKNKDYYLCQEE"
     gene            complement(4643..4789)
                     /locus_tag="ACHKAU_003524"
     CDS             complement(4643..4789)
                     /locus_tag="ACHKAU_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532516.1"
                     /translation="MHTVFELWVRKTFGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LNVV"
     gene            complement(4793..5035)
                     /locus_tag="ACHKAU_003525"
     CDS             complement(4793..5035)
                     /locus_tag="ACHKAU_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5532517.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRCTDRSVFFRRMDGHSGRVCIG
                     EFNCLFEHIDHQQYCKILADTEQEKHLKKLRAMQRR"
     gene            complement(5120..5284)
                     /locus_tag="ACHKAU_003526"
     CDS             complement(5120..5284)
                     /locus_tag="ACHKAU_003526"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5532518.1"
                     /translation="MPNDAQWCVVNTEYGYYVQCWSEGQGWLGDDISIPECDVTHWMP
                     LPEPPQEVSQ"
     gene            complement(5372..5635)
                     /locus_tag="ACHKAU_003527"
                     /pseudo
     CDS             complement(5372..5635)
                     /locus_tag="ACHKAU_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(5858..5983)
                     /locus_tag="ACHKAU_003528"
                     /pseudo
     CDS             complement(5858..5983)
                     /locus_tag="ACHKAU_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(5985..6314)
                     /locus_tag="ACHKAU_003529"
     CDS             complement(5985..6314)
                     /locus_tag="ACHKAU_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5532519.1"
                     /translation="MSKIDYQALREAAEAIKVVATPQKLLAFRMKVTPQVVLVLLDEL
                     EAKNKRITELEAREVQLPTRYDLRYGHPINADKRHVMIPKENGSWLCLIDLEHALRVA
                     GIRIKGE"
     gene            complement(6311..6550)
                     /locus_tag="ACHKAU_003530"
     CDS             complement(6311..6550)
                     /locus_tag="ACHKAU_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532520.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTREAFFSRIAAQIRKGGNQ"
     gene            complement(6543..6746)
                     /locus_tag="ACHKAU_003531"
     CDS             complement(6543..6746)
                     /locus_tag="ACHKAU_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532521.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(6743..7621)
                     /locus_tag="ACHKAU_003532"
     CDS             complement(6743..7621)
                     /locus_tag="ACHKAU_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFO5532522.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVLKFWGIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGRGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWQKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYRSLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(7612..8148)
                     /gene="yfdR"
                     /locus_tag="ACHKAU_003533"
     CDS             complement(7612..8148)
                     /gene="yfdR"
                     /locus_tag="ACHKAU_003533"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5532523.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(8277..9101)
                     /gene="yfdQ"
                     /locus_tag="ACHKAU_003534"
     CDS             complement(8277..9101)
                     /gene="yfdQ"
                     /locus_tag="ACHKAU_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO5532524.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISNIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAMQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(9167..9529)
                     /gene="yfdP"
                     /locus_tag="ACHKAU_003535"
     CDS             complement(9167..9529)
                     /gene="yfdP"
                     /locus_tag="ACHKAU_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO5532525.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(10196..10870)
                     /gene="ymfK"
                     /locus_tag="ACHKAU_003536"
     CDS             complement(10196..10870)
                     /gene="ymfK"
                     /locus_tag="ACHKAU_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFO5532526.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            10961..11161
                     /gene="ymfT"
                     /locus_tag="ACHKAU_003537"
     CDS             10961..11161
                     /gene="ymfT"
                     /locus_tag="ACHKAU_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO5532527.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            11205..11756
                     /gene="ymfL"
                     /locus_tag="ACHKAU_003538"
     CDS             11205..11756
                     /gene="ymfL"
                     /locus_tag="ACHKAU_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO5532528.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            11902..12081
                     /locus_tag="ACHKAU_003539"
     CDS             11902..12081
                     /locus_tag="ACHKAU_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5532529.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            12011..12904
                     /locus_tag="ACHKAU_003540"
     CDS             12011..12904
                     /locus_tag="ACHKAU_003540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5532530.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGRG
                     NPEFTKAHNRALAVFLCHEQHYAQIMVGRAGPTSVGPGSLVTGISTPVRLTTNKVVES
                     LGGELLKITKEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTL
                     ECSASFTARNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFN
                     QMEKQLSKLAVPSDVAHNASVLYSYISSIHQVWLQQLYPMLEKAESPLAVSLYDRIND
                     AAFLARLIHSSLNSSEVRGRK"
     gene            12901..13125
                     /locus_tag="ACHKAU_003541"
     CDS             12901..13125
                     /locus_tag="ACHKAU_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532531.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            13122..13940
                     /locus_tag="ACHKAU_003542"
     CDS             13122..13940
                     /locus_tag="ACHKAU_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5532532.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARRDDPYFKASYDNVDYSQIPAGFRG"
     gene            13937..14431
                     /gene="yfdN"
                     /locus_tag="ACHKAU_003543"
     CDS             13937..14431
                     /gene="yfdN"
                     /locus_tag="ACHKAU_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5532533.1"
                     /translation="MIMSLLNDVQKFIEAHPGCASGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRQTEVLQESEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            14431..15084
                     /locus_tag="ACHKAU_003544"
     CDS             14431..15084
                     /locus_tag="ACHKAU_003544"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5532534.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASSMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            15081..15407
                     /locus_tag="ACHKAU_003545"
     CDS             15081..15407
                     /locus_tag="ACHKAU_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO5532535.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            15404..15793
                     /locus_tag="ACHKAU_003546"
     CDS             15404..15793
                     /locus_tag="ACHKAU_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5532536.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            15813..16610
                     /locus_tag="ACHKAU_003547"
     CDS             15813..16610
                     /locus_tag="ACHKAU_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO5532537.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNSQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            16618..17607
                     /locus_tag="ACHKAU_003548"
     CDS             16618..17607
                     /locus_tag="ACHKAU_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5532538.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLERWLLREKGCQWPHSDWHSE
                     NMTTMRHTPGAIRLCWHCDNQLRDQFTERLESMATDNCTRWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQCG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
BASE COUNT         4378 a         4219 c         4300 g         4748 t
ORIGIN      
        1 ataactttaa atttaatgct tcaggatttt cactgagaaa gtgaaaggca taaactaagt
       61 atacagctaa agtagccgtt ccaattgttg gtccaatatc gccttgatca tgaggttcaa
      121 ggtttacatt gaacttgaag attagccatt caaatcccca agtaaaaaaa acaactaata
      181 tcatggctgc gaataatttc gcgatgttat ctgctgatgg attcatcaga acagattgtt
      241 tctgaaaaaa ttcacaaagt gagagtccga aaaaaataaa aaaaagtggt ctggaactaa
      301 atttttcaaa aactttgaat agggtttcca ttttctttac cttcaggatt atattagcta
      361 ttctgatact taggctacca gagcatttga tctttgagct tgaacgtaat cactccacca
      421 ttgcatcaaa ctctgtcgct ctatcagata ttctgcacga ttgtatgctg cgataatttc
      481 atcttttttc gagtgggcaa gcgctgcctc aagaacttca gctctgaatt taccagactc
      541 ctctgccgct gttcgtgcaa tagaacgcat accgtgagct acaagctcgc ctccgaaccc
      601 cattcggatg atagctgcgt tggctgtttg ttcatgcata tgattaagag gcgcttttat
      661 gctggggaaa acccattctc tatgcccact tattgatttc attaattcaa ggatgcgcaa
      721 agcttcttta ctcaaaggaa ctttgtgaag ctttttcatt ttcatgaaat cagcaggaat
      781 gttccaaatg ctgttggttg tatcaatatc agaccacctt gcgcgaacgg cttcacccgg
      841 acgaacccat gtcaacaatt gccattcaat tagcatacgt gtttccaacc ggattgacgc
      901 attcgtcaaa gattccataa accttggcaa ttcgcttggg ggaagggcag gcatattttg
      961 cttttttggt ttactgaatc tttgaccaag gttgtcagcc gggttgaact caataagttc
     1021 ttcagtagct gcccaccgga agatttcatt cagacgtgaa atgatacggc gtagagtttc
     1081 caatacgcct cgttgctcaa taggatcaag gtgttgtttt aagagcttag gtcggatctc
     1141 attgataggg acattaccca gaccgggaaa gacatttctc tctaagctgc gccagatgtc
     1201 tgctgcatgg tcttgtgaga tacctgatgt ctttaccttc tcatctaacc atttccgcgc
     1261 tacggcttgg agagtgtgct cagtagcact ctttaatgcc ttcgccttat cgttgttatg
     1321 gatttgggga tcaacaccat ttgccagaaa ggagagatat tcatcacgta aggctctggc
     1381 tctggctctt gcaagggtaa ggtgaggata tgtcccaagg ctcattttgg ttcttttctt
     1441 gctcacgggt actgcatacc tgaaatacca atttttcttg cctcctttcg ccaaaggagc
     1501 gattcgtaga atcagaccat caccgtcaaa caagttgatt tctttatcgg ctggcttggt
     1561 gcttttgatt tcagtgtcag tgagcttctt agcgattttt gccattttgg gaccctcggt
     1621 ttttggaccc ttcttagtgg gtcccattca gggtgccata actcgtagtt ctcagcaatt
     1681 ctcactggac gacaatagac gtaaaaaagc ccgcagagct tgtgctgtgt gggcttagta
     1741 gacttcattg aacttcaaac aactaaaaag tggagctggc gggagttgaa cccgtgcccg
     1801 agcaatgctt aacccattga ataacaatgc attctctgtt taaaaatgcc tcaagtgcat
     1861 tttgagtgca ttttctggtc ctctcttggt ccatacaacg agattaagtg gccacttgaa
     1921 gttccggccc tgcgtcgcta tctattaacg gctgaccttg aaccattttt tcaaccatat
     1981 cgtttgaagg atatggaacc ccgagctcaa ccgtattagg gttggaacgg tgggtttcaa
     2041 caaacgatgt tccgttccat ttttttgaaa ggtagattat tccttcaaag gctgcagggt
     2101 cctgtttctg agactctaag atcatgtgga agccacgacg ttctatcccg cgtaagaagc
     2161 tggagaagca ctccctcagt aattcgtcat ccaaatcctt gaacttgata ccctgcgcaa
     2221 gaaactcgaa ataattaaga atatataaaa ggccattgat ggcatccctg tactcatcgg
     2281 ggaccttcat attcttgtat tcttctttgt ctgggttgca gcgccactct gatagttctt
     2341 gtggtacata acgcatgccc cgatagaatt tggtgctgtt ccgaagctgt ttttggtact
     2401 ccgggcttgt tcgggtgtta atgatcatgt tcagcgtgtg ggacctgcgc gtgttagcag
     2461 cggacgttat gcattgtatc caccatccca ttccaacgag cattcctgta accatgatcg
     2521 aaagcgatgt ttgataggcc ggtgtgtacc ttaaaattgc atatccaata aggataatca
     2581 cataacaaac agtgctgaac ataggaagaa attctgcagt gtctggatag cgttttttga
     2641 tgaagcagaa gatagaagat attgctgcac cagaaccagc aatgacgatc agccaatcaa
     2701 tgatggcgat gctaaatggt agttttccag ggtagatata agtaaaaagt gctctgacaa
     2761 tcatcattag agcaaaaatt gtagcgctga cgttgactaa tgttctcgcc atcttattga
     2821 ttcctataac aaaagacctc cgcatgggag gccttattgt gctaatggag gtttgtttta
     2881 aaattttagg ttgtagttaa ccttcaccga aacctacggt cagtgtcttt ttcatagtgt
     2941 tcactccagt tgtggtgctt cgccgatacc tacgttaagc tcttgaaaag taaaaaaaca
     3001 taaaagtttt agaatttgtg gagcacagga taacagtttg tactgggact catcaagtac
     3061 tatcagtgtc cttgcactgt cctagtgaaa atttattgca ataaagtaac ttacgtgaag
     3121 tcaagaaata tatttgcact ccatttcttg ctatgatgta agttattgat tttaattaaa
     3181 tttacaatta cctaaatgaa ctatggaaac tatcaataac atagatgata gctcatttca
     3241 caaaaagcaa tggtagactc attcttaatt ttcatcagcc ttaagcctat gtactcagct
     3301 tgacacaaca tttatagacg ttttaccatc gtattcagcc aggtatttac cgtaattgcg
     3361 gaacagcatt tccggcccct tatgccccat ctgcccggca agccagaaaa ggtttacacc
     3421 ctggctaatg tgtctggtgg cgaatgtgtg gcgcgtctgg tacgggttac ggtagcgcac
     3481 accagctttt ttcagggtcg gcacccatgc ttttttacgg atagcgtcgg cgttcgtcca
     3541 gggttctccc gttttcggat cgctgaatat gaactcactt ttcataaagg tgtattgctt
     3601 ctgcgcctgc agggctgcca gcgcctcact gttcagctcc accttacggg taccggcttt
     3661 tgtcttggtg cctttaagta cccctacgac actggccgcc tgaacgtggg ctgtgttcgc
     3721 gatggtgtcg agatcagccc agcgcagcgc gcacagttct gagctccgca gacctgtatt
     3781 gaaagcaaag cggaacaggt tttcccattc cctgtacctg cagctctggt aaatggcaag
     3841 ggtctccgct ggcgtgaacg ggtcaacctc gtaatcgtcg gcgctcgggc tactgtcgat
     3901 cacgtggtac cggctggcgc tgacgagggt taccgggtta atggtcagca ggccatccgt
     3961 aacagcttca tcaatggcgc tgcgcagaaa tgacaggtta ttcctgattg ttttcagctt
     4021 agtttttcgg ctggctatcc aattttttag tatcgcaggc gtcaattctg ttacatgtag
     4081 tttatgtagt tctgatagtg ctgatagaca tttttcgtaa ccgccaatgg ttgacggcga
     4141 cagattacgg tttatgcaaa ttttcaggta ttcatcaaga taggacttaa tatttttggt
     4201 tttctttact acaccgaata actccagttt tttggaattg gggaaatatt tcgcatattc
     4261 aaacgttccg ctggcgatct gattttgtat ctcccctagc aggcgctcag catattttat
     4321 accacgtgta tttgcctcaa gccgagatag gggctccctg caaagaaccc ctttatatgt
     4381 gaaagtgata actagtgttg aagcagtttt atgcttacga atagttactc ctcttggcag
     4441 agataataat ccttgttctt tcttgcccat tttgaaacct ctattaagtc gacccaacgc
     4501 tctttaaccc cgtcgacttt taatacatgt accccttctt tccatattcc tctttgtatc
     4561 cgtttgttaa cggcatcaac cgtttctccc gcgttgcggc agtaggttga tataggtacg
     4621 caatccagcc ccacagttca cctcacacaa cattcagccc acgacagtga caccacactt
     4681 caaacattcg tttcacaatt tcacgacagt agaaaccgtc aacatctcgc gtcaggtcat
     4741 agcgattgcc gaatgtcttg cgaacccata actcaaaaac cgtatgcatg tatcacctcc
     4801 gttgcattgc gcgcaatttt ttcaggtgct tttcctgctc agtgtccgcc aggattttgc
     4861 aatactgctg gtggtcaata tgttcgaaca ggcagttgaa ctcaccaatg catacccgcc
     4921 cggagtgtcc gtccatacgt cgaaagaaca cagagcggtc cgtacagcga gtaatcacca
     4981 cagggtatcc ggccctgtcc gtgtatattt gcccgcgttg aataagtgcg aacatgagat
     5041 tatccccagc ggcaaatcga aaaaataatc agcgcaatcg atattgcaac acctacaatt
     5101 gcaaatgctt caggccaact cattgactca cctcctgcgg cggttctggt agaggcatcc
     5161 agtgggttac atcgcattca gggatgctga tatcatcacc aagccaccct tgaccttcag
     5221 accagcattg cacgtaatac ccgtattctg tgttcactac gcaccactgt gcgtcgttcg
     5281 gcattagctc actacagctt atccaaccat ccggagttac cggaacttgc ggaatggctg
     5341 tctgctctcg aacgtcatta ggcgctatag gttctgctgc caactgactg gcatatttgt
     5401 taatggtaac gataagctct tgctcagcct catccagata atcaccgata cctcgcctgt
     5461 caccgtcaaa atcatcgaaa tcggcacgaa tcttggcaac cttctggatt gcggacaaca
     5521 cctcactagg aattaccgga tagttggttg acgtttccgc gatttcccga aaattattgg
     5581 ttgacgaatt cttattttcc cgaaagtttc tggcctgaag catggcggcg cggcaggcat
     5641 tccagccagt agcgtaatat ttcgcatcct gccagctcgt ttgtacattt gagtacgctt
     5701 tttcctctgt catggcctct ggtattgatg ccggtgcaat tcgtggcagg ccgatatata
     5761 gtggtacatc tcccggctcc atcgaattga cgggccaaat atacgcatca ctaccatggt
     5821 taatgagcgc aatccccgaa gtatcgatga tgccaaatgg ctccgctgtc aatgcagtca
     5881 atgcaatttc ataagcccga cgctcaatat tgtctcgcac gtccaggctg cttatgcgct
     5941 ctttgatttc tttaatcagt ttcttatcgg tgaaagtggt catctcactc tcctttgatg
     6001 cgaatgccag cgacgcgtag tgcgtgttct aagtcaatca ggcaaagcca gctgccattt
     6061 tctttaggta tcatgacatg tcgcttatca gcatttattg ggtgcccata tcgaaggtcg
     6121 tagcgagtcg gtaattgaac ttccctcgct tccagttctg taatgcgttt gtttttagct
     6181 tctagttcat ccagcagcac cagcacaacc tgcggtgtga ctttcatacg aaacgcaagc
     6241 aatttttgcg gcgttgccac gacttttatt gcttctgccg cctcacgcag tgcctgatag
     6301 tcaatcttgc tcactggttg cctcctttac ggatctgcgc tgcgatgcgc gaaaaaaaag
     6361 cctcccgcgt atggctgtta agagctggcg cgaacgccgc gttaagaacg gcagcatcac
     6421 agccgtcatc gatatagagc gcaatttttt tctccaggcg cgctttggct tcctgcaact
     6481 gcataccccg gcacgcacgc gggatataat cagcaatttg agcgatagct ttttcgttct
     6541 gtttaaacat gcttcacctc gataggcttg atggtgtcta acagcagtcg gcggcgcgta
     6601 ttttctgcaa aatggcggcg tccagtttct ttgtggtaaa actcgttttt gccaacgacc
     6661 cacatccgct ctgtttggtg caattttttt acctgcgggc catctttggt gatcacggta
     6721 ccggtatggg tttttacgat tgtcatgcca cgacctcttc gaatttcaac tccaattggt
     6781 caccccagat ttcacatgat tcggaacacg aaccagtatc aaaccgcttg gccagcacca
     6841 tcgcctgata caaacttctg tagtcgctgt cggcatacat tctggcaatc ccgtcaagcg
     6901 tcaggtgacc acggtacata acgtctttac ctgttctgcg atgaccatcc ctgacgtgtt
     6961 tgcctgtaac cagttcatta aaaacccgca tcagaccagg ttcgtcttta catgcaagcc
     7021 ccagcttttg cgttgacttt ttgatgcaaa aaacacagtt cccgaggtgc tccgggattt
     7081 gcaaatcaaa aggttgtttt tgccaccacc ggataacatc cgacttatca aaatctgaca
     7141 gctcggcaag ataccggacg ccaggtttcg gtttcagcct acggggttcg tccgcacgaa
     7201 taccgagcca tgtgatgtaa ttccctcgcc caaaatggtc atcgcaatat ttcgtgaaag
     7261 gggtgagttt tagcctgtca gtacagaatg cgccgccgat gtatggcgta ccgtactttt
     7321 taaccatgtc cataaacggt ttaagcaccg gcattcgcgt ctgaatatcc tttggttccc
     7381 attccgtata accatttggc tgcccaagct ctggatttat atcgacctgc aacacagtta
     7441 gcggtatgcc ccagaacttc aaaacctccc gaataaagcg gtatgtcagc ggatgttcgc
     7501 aaccggtatc cataaagatg tagcagacgt tattgccagc ctttctttgt tcttccatca
     7561 ggtgaacaag atatgcagat gttctcccgc cagaaaaact aactacatga gttatgcaca
     7621 tttgcgtaat tccgataact cgttgaagcg ttccataaac atcccgtagg catggcccgg
     7681 tgccagtgga atcactttga acatctctgt tgccgggata ccttccaata caggccagaa
     7741 agagccatca tcaagtccga gatcgcggcg ttcggttgcc agcatgatga gatcggcata
     7801 tttcactggc gtgctcataa caggaggtaa cccgtatttc tcacggatta cggcgtctat
     7861 tttttcttcc atccgtttat agtcaggaag aagtcgtttc agtggcgcgg ggatgtcctg
     7921 gcaatatgct tctgttgcat catgcattaa cgcttcaaaa gcaaattcct gcggcaccag
     7981 ctggctgcaa agcaccgcat gctgggcgac actgtagaag tgtgaaagat gtcctgcaaa
     8041 gcgacagata tttgaaaggg aaactgcgat atcgttaatc acgatgtcgt ctttatttat
     8101 cttgtcataa taaaaatgct tcccggaaaa agttttaata aatgacattt cgttctccac
     8161 tttatatgcg ctgcaccgcg ctgaattctg ctaaaaggaa gctctcacca tccggtgatt
     8221 attgagttaa ttacgtttcc ataaatgccc ccgcaggggc atttgcagta atgaaatcag
     8281 gcggtgaaag taccaataaa ggtttctact ttgctgtctt tgaatttctc aacaagcaga
     8341 tcacgaaatt cgttagccat ttcttcctgc atcgcttcca gctgaataat gcgcagaacc
     8401 agtacaggac gatcgccagt gataatgctg aggcgtaatt taaacggacg ttctttcagg
     8461 ccttcaaacg gaacgcattt aaattcaaat gccactggca taatgtcttt ggtcttcgct
     8521 tcgacagact ccatcaggga gcgtttgccg ctgaagtcat tgtcttcaaa atcagcggtc
     8581 tggtttgctt caattgtgat tttacggact gccgcagccg ctttggttgc ctgaatggtg
     8641 tcaccattag catcaaagcc cacaaggtag tcggcccagt cttcaatcca ttctgccagt
     8701 gacttctggg agttacgctc gccattaaca gacaacagag cagaaaacgg tgctgtcttt
     8761 ttcagtttga gggtggcggt gttatctgcg tgacctggtt catcaatagt acccaggtta
     8821 agcacactga cggctcgcat attatcggca tcgataaagc agcgggtgcc ttcatctgca
     8881 agatctttag aataacgggt aaagtcatcg atgctggcag tggaaagcgc accacggaaa
     8941 cggaagcgat ttaaattaaa tttttccaga tcatgaatgc ggaaattctc aggcaatgcc
     9001 acagcatcgg caccaatatt actgataatt tcattaacac cctgagcaga aataagggca
     9061 tggatttgat taattgcggt tgcgtctaag ttctgagaca taataagtcc tcactatata
     9121 aagatattca gtgatgagat aaataatcag ttaattaaga acgatattaa tgacctgctg
     9181 cgcggagttt tccgtcaggt tcaccggcaa gagtcagtaa ttgtccctgg tcttcctgca
     9241 gaatagtcag tcgaccaccg cgattgacat acatcggcgt ttcggtggtg tcttcttcgg
     9301 aaattttccc gcggttagtc gggcgaacat atgagagttt gtgtttgatt ttcacacggt
     9361 tctcatcaaa cggttcgatt tccaggttga gcgagacctt acctttggtt ttcgtgttca
     9421 tcacaccgga agcgacttca ctgagaactg cgccgatttt ggtttcaaat acgccgccgt
     9481 ccagctcccc gataaatgcc tgcacatcag tactgcgttc gctagccatt ttgctgctcc
     9541 tcatcatatc gaccctgcaa ggtcggttgg tttctccaca aaacagagaa gaacacctgc
     9601 ggtggcagcc gcccggctgg attgggttat gagcccgtcg tccggtgatg ctcttctctg
     9661 ttttgtaaaa agagcggtac cagccggaag caagtgtaca aactggtacc gccaaagcag
     9721 tggctgttgt ggtggggttg tcactcaggc gtatggtcaa cctgacaatc cggtgtcctc
     9781 aacggggaaa gagtaacccc gccatactta ccgccgcgcc atttcgcgga ttaccacaac
     9841 gctgagagca cttagcaagt tacggcacca cactttgtcg cggttccata aatgccctca
     9901 tcgttgcacc ctggtctctt cccaggcgtc aaaccggatc gccgcgctgg ttaggcgtct
     9961 tatcagcatc atcattgact tgcacattcc ggctacctgg tttttttgcc cgagcaagga
    10021 gtggattgtc ccctttaacg tccccagacc gctaacgacg catgtgccat acgccgtgtt
    10081 acaaccaaat tttgtttaat cttgcctgcg gcatgtttct tttagataca ttatgtatct
    10141 caagggtaca ttgtcaagta taaaaaaacc tgccgaagca ggttataaat attgattagg
    10201 cctttatttt gtatcttctt ggttttcctg agaaaattac tgtaccaatt atagagcaat
    10261 taccgttaat cttaatgtaa ggctcaggcc agtttgggtt taatgctttg agataacgct
    10321 gtgttccatc ttctatcaat cgcttgaagg tggtttcgcc tgtatcgtgc atcaatgcaa
    10381 taacgtcgtc accgtggcag gcagggactt cgggatcaac aaaaatcatg tctcccgggc
    10441 ggtactcatc aatcattgaa tcaccaatca cccgcaagat ataagtcatt tcgccacagg
    10501 gtacagggca gggataagtt tctgctgtgc tcaaatcaac ctcagaatag ccaacttctt
    10561 tccatgctcc ggcctgtacc catgatatga cagggactaa cgttatttgt ttgttagtga
    10621 ttgaaacatc aggttttttt gtgatgttcg tggtctggtg ttcttgatca agccatccga
    10681 caggcaggtc gaaacatttt tcgatgtgcc gtgccatgct gtcaccgata tttttagtag
    10741 caccatctcc cataaacctg ctggtctggg ttggctcgcg atcaatcatg gtggcaaagg
    10801 aagaattccc gccaacacca tctctcagtt ttctggcgtt agaccgccgg atgtcatgga
    10861 ctgttttcat aaagaaatta aaacctttgt accgataagg tacaagtatc ttgaaggttc
    10921 atattaatca tgtaatatgt atattggagg tacatattgt atgaaagcgt attgggactc
    10981 tttaaccaaa gaacagcagg gcgagttggc cggaaaagtt ggctcaacac caggctactt
    11041 acggctggtt ttcaatggtt ataaaaaagc cagttttgtg ctggctaaaa aacttgagca
    11101 atgcacgtca ggtgcaatta cgaaatctga cttaagaccg gatatctatc cgaaagatta
    11161 acagaacacc ttcaattttt aaccacagaa cgatgaggct aaccgtgggt aagtatcact
    11221 ggaaagtaga aaaacagcct gagtggtacg tgaaagctgt cagaaaaact atcgcagcgt
    11281 tgccgggtgg ttacgctgaa gcagctgact ggctggatgt aacagagaac gcattattta
    11341 accgccttcg tgccgatggc gatcagattt tcccgctggg atgggcaatg gttttacagc
    11401 gcgcggctgg cactcactac attgcggatg ctgtcgcaca gtctgctggt ggggtgttcg
    11461 tatcgcttcc tgaaattgag gaagtagaga acgccgatat aaaccagcgc ctgctggaag
    11521 tcatcgaaca gatcgggaat tactcaaagc agattcgttc ggcaatcgaa gatggggtcg
    11581 tggagccaca cgagcagaca gcaattaatg atgagttgta tctgtcaatt tcgaagctcc
    11641 aggagcatgc agcactggtc tacaaaatct tttgcgctcc agaaaagagt gacgcccgcg
    11701 agtgtgcagc tccgggcgtc gtggcgtttt gtgtctgtgg agaaactaac gcatgaacag
    11761 tttaacggca aataaccgtt tgtcgcaaca gctggtggtc agtgtcgctg aacacctgtt
    11821 gttacggcat gaatgcagat taccaaatca cctggctgta agtaaccaca gagaacttta
    11881 cctgactgtg gggggcgagt tgtgcaggaa cttaaccgct ggtttcgtga cggaagaggg
    11941 ctttatgtcc atgttattcg ttgggagcca gaaacacagc gcgttatcta tcttcgcaaa
    12001 gactacccgc atgagtgctt tagtcctttg tggaaattca ggcgtgattt tgttgagtgt
    12061 gaaggaccac cagcacattg attctgccat tccgggacgt tacactgttc aggcacctta
    12121 taaagcgggt gccgggcgtg gaaacccgga attcaccaaa gcgcacaacc gcgctcttgc
    12181 ggtttttttg tgtcatgagc agcattacgc ccaaattatg gtggggcgtg cagggccaac
    12241 ttcggttggg ccgggttctt tggtgaccgg tatttccacc cctgtacgtc tcaccaccaa
    12301 taaggtcgtg gaaagccttg gtggtgagtt attaaaaatc accaaagagg ctgccatcat
    12361 ggctacgatc ccaaccctca ctcaacccga aattgccatc gttgatggtc aggctgttac
    12421 ttcatccctg gctgttgcca acttcttctc caaacgtcat gacgatgttc tgaaaaagat
    12481 ccgcacgctt gaatgctccg catcattcac tgcccgcaat ttttcggtga gtgattacac
    12541 cgattgcaca ggccgcaaac taccttgcta tcaaataacc cgcgacggct ttgcgtttct
    12601 tgctatgggt ttcacgggta aacgtgctgc ccagttcaaa gaggcataca tcaatgcctt
    12661 taaccagatg gagaaacagc tttcaaagct cgctgtaccg agtgacgttg cacataacgc
    12721 cagcgttctc tattcctaca tttcatcaat tcatcaggtc tggttgcagc agctttatcc
    12781 catgctggaa aaagctgaat caccgctggc tgtaagtctg tatgaccgaa ttaacgatgc
    12841 ggcatttctt gcccgtctta ttcattcgtc gctgaactct tcagaggtaa gggggcgcaa
    12901 atgatccgaa atattttcaa acggtttacc aatcaaactt tccgttgtcc tcgcccgggt
    12961 cagtggtaca ccacgcctgc agggcatgtt ctacgtgtta gcctggttga ccgtgaatgt
    13021 cagaaggtga tttgtgaacc gctgggtcgt aattaccgcg tcagtatgcc gctcatagcc
    13081 tttcgctccg gaaaaaacat gaagcatctc ggaggtgcag catgagtatg gagctgatgg
    13141 ttaaagcgat gaaaattcga gtgggtaatc cattgcgaaa actggttctg atcaagctgg
    13201 ctgataatgc cagcgatcag ggtgagtgct ggcccagcta ccagcatatt gctgaccagt
    13261 gcgagattag caaacgttct gtgatgaatc atattgcggc cctttgtgag tccgggctgg
    13321 taaaaaaagt cacccggaaa ggtgaaaaag gtaactcaag taatatctat ctccttcatc
    13381 tggatggtgc aggagattca ctagggggta gtgcaaataa ttcactatct ggtgcagcaa
    13441 attcaccagg tagtgcagga gttgcaccag ggggtagtgc aggagattca cccagaacca
    13501 gtcactcttt tgaaccagtc aaagaaccag tcaatgaacc aatagctgtt ggtgcatctg
    13561 ctgatgagtc tgtgcgagtt cgttcaaacc gaccggaata ctctccggag tttgagcagg
    13621 catggctggc ataccccaaa cgtgctggtg gcaattcaaa atcagcagcc ttcaaagcct
    13681 ggaaagcccg tttgaacgag ggggtaaacc ccgaaaccat gctggaaggt gtgaaacgct
    13741 acgcgggctg ggtatctgcg atgggcaata gcggcacaca atttgtgaaa caggctgtca
    13801 cgttctttgg tccggatcgt catttcgaag aatcctggga agttcctgcg gtatctgcag
    13861 ccagacgcga tgacccgtac ttcaaagcca gttacgacaa cgtggactac agccagatcc
    13921 cggcaggatt cagggggtga tcatgagtct gttaaatgac gttcagaaat tcattgaagc
    13981 ccatccgggg tgtgcttccg gagacattgc ggatgctttt gcaggttact cacgacagcg
    14041 cgttctgcag tcagcaagca agttacgtca gagtgggcgt gtggctcacc gttgtgaagg
    14101 ggatactcgc agacatttcc cgcgccagac tgaggttttg caggaatcgg aaccgcaacc
    14161 agttcgtgaa accagacctg tgcgcaattt ctatgtcggc actaacgacc cccgggtgat
    14221 tttgtgcctg acccgccagg ctgaagaact ggagtccagg ggcttatacc gtcgtgctgc
    14281 aacggtgtgg atggcggcat tccgtgaaag ccactcccag ccagaacgaa acaattttct
    14341 ggcgcgtcgt gagcggtgct tacggaaaag cagcaagcgc gctgcatcgg gtgaagagtg
    14401 gtatctgtca gggaattacg tgggggctta atgagtaata aatattgcca ggcgctggtg
    14461 gaactgcgga acaaaccagc ccatgaactg aaggaagtgg gcgatcagtg gcgcacgccg
    14521 gacaacattt tctggggaat taacaccctg tttggcccgt ttgttctgga tctgtttact
    14581 gacggtgata acgccaaatg tgccgcgtat tacacggcgg aagataacgc gctggcgcat
    14641 gactggtcag aacgtcttgc ggagcttaaa ggtgctgcct ttggtaatcc cccatacagc
    14701 cgcgccagtc agcatgaggg gcaatacatc accggcatgc gttacatcat gaaacatgcc
    14761 agttccatgc gtgataaggg cgggcgctat gttttcctga tcaaagctgc caccagcgaa
    14821 gtgtggtggc cggaagatgc ggaccatatt gcttttattc gcgggcgtat tggttttgaa
    14881 ctgcctgcct ggtttatccc gaaggacgag aagcaggtgc cgacaggagc ttttttcgct
    14941 ggtgctattg ctgtttttga caagacctgg aagggaccgg caatcagcta catcgggcgc
    15001 gatgaacttg aggcatgtgg tgaggcgttt ctggcgcagg ttcgccagca ggcggaaaaa
    15061 ctggtcaggg agatggcggc atgacgacgt taactcaatg ccagcagcag gtgctggata
    15121 tgctgatttc ttatcagaaa gaacgtggct tcccgccaac caatcaggag gtggcaacca
    15181 tgctgggata ccgttcggtg aatgcagcgg tggagcatct tcgcgcactg gagaaaaaag
    15241 gcgtcatcac gataaagcgt ggtgtggccc gggggatcac gcttcatacc gcggtgaagg
    15301 acgacgacag cgaggcggtc gggattatcc gctcactgct tgccggtgag gaaaacgcaa
    15361 ggctgcgtgc aactcactgg ttacatgaga gaggcctgaa agtatgaagc tgatcctgcc
    15421 tttcccgccc agcgtgaaca cgtactggcg acaccccaac aaaggggcat ttgctggtaa
    15481 gagcctgata agcgaggcgg ggcgaaaatt tcagagcgcg gcgtgcgcag caatagttga
    15541 gcagttacgt cgtctgccga aaccaacgtc ggcacctgct tcagtggaga tcgtgttgtt
    15601 tcctccggat aaccggatcc gcgatctgga caactataac aaggcgctgt ttgacgccct
    15661 gacccacgcg ggtgtgtggg aagacgacag ccaggtgaaa agaatgctgg tggagtgggg
    15721 accggttatc ccgaaaggga aggtcgagat cactatcagt aagtacgaga aaacggcggg
    15781 tgcagccgcc tgatcaagag gagaaacgaa gtatgaataa tctgatggtc attgatggta
    15841 ttgaagttcg tcgtgatgct tatggtcgtt acagcctgaa cgatttgcac agggctgccg
    15901 gttctctgga taagcataag cctgcattct ggctccgcaa tgagcaaact gaacgtttaa
    15961 taagcgagtt gcagatttgc aactcggtca atatagagcc agttaacgtt attcgtggcg
    16021 gaaatagcca ggggacgtat gtctgcaaag aactggtgta tgcctatgca atgtggatca
    16081 gcccgtcatt ccatctgaag gtgatccgta ctttcgacat ggtaaccagc gcaccggaaa
    16141 aattatccgg acaggctgct gacaagatgc aggctggtgt gatcctgctg gactttatgc
    16201 gccgggaatt aaacctgtct aactcatcag tgcttggagc ctgtcagaaa ctccaggagg
    16261 ctgttggctt accgaatctg gcaccgcgct atgccattga tgctcctgct gacgcgcctg
    16321 atggctcaag ccgccccacg ctgtcactga gtgcactgct gaagcagtat ggtatccgcc
    16381 tgacagctaa tcaggcatat caccagatgg cgaagctggg gatcgttgaa caacgtgaac
    16441 gatacagccg cactgcgatt aacaacatca aaaaattctg gtcgctgacg gcgaaaggct
    16501 gcatgttcgg caagaacatc accagtcccg caaatccgcg cgagacgcag ccgcatttct
    16561 tcgaatcccg gttccctgag ctgttaaagc tgctcgatac cgttcattga ggtgaccgtg
    16621 agagcgctac tgacccctga aattgccccg cgtatgggga tcgtattgtt caggcccggt
    16681 tcagagctga tgcccctgtt tatgcagggg cgtgtcctgc tggagcctga gccggaacgt
    16741 tattcatctt tcgccagtgg tgccgttccg gcggcatcac aaccgctggc ggatgatcct
    16801 gccgttcggg ccgtgttccg caatgaggca gtgattcgtc gtgctggtgg agtggaatgt
    16861 cttgaacgct ggttacttcg tgaaaaaggt tgccagtggc ctcattccga ctggcacagc
    16921 gagaacatga ccacaatgcg acacacgccg ggcgcaatcc gtctgtgctg gcactgcgat
    16981 aaccagctgc gcgatcagtt cacggaacgg ctggaatcaa tggcaacgga taactgtacc
    17041 cgctgggtgt tgtctgttgt gcgtcgggat ctcggttttg atgacagtca cgttgtgaca
    17101 atgccggaac tgtgctggtg gctgattcgt aatgacctgg cggatgcctt accggaaagt
    17161 gcagcccgta aggcactgag attaccgaag cctgttgtgc cgtctgtcac ccgggaaagt
    17221 gaccttgtgc cttcggttcc tgccaccagc atcatccagg ataaagcgaa aaaggtgctg
    17281 gcgctgaaag tggatccgga gtcgccggag tcttttatgt tacgcccaaa acgtcgccgc
    17341 tgggttaatg aaaagtacac gcgctgggtt aagacacagc cgtgtgcatg ttgtggaaag
    17401 cccgctgatg atccccacca cctgataggc cacggtcagt gtggaatggg tacaaaagcg
    17461 catgaccttt ttgtgttgcc tttgtgcaga aagcatcacg acgagctgca tgcggatacc
    17521 gtggcatttg aagagaagta tggctctcag ctggagctga tatttcgttt tatcgatcgt
    17581 gcgctggcaa ttggcgtact ggcgtaagtg gagaacgagc atgaaccttg aagccttacc
    17641 gaaat
//
LOCUS       DAZFJF010000096        17579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFJF010000096 DAZFJF010000000
VERSION     DAZFJF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.096"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(153..2786)
                     /gene="mngB"
                     /locus_tag="ACHKAU_003549"
     CDS             complement(153..2786)
                     /gene="mngB"
                     /locus_tag="ACHKAU_003549"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO5532539.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(2804..4720)
                     /gene="mngA"
                     /locus_tag="ACHKAU_003550"
     CDS             complement(2804..4720)
                     /gene="mngA"
                     /locus_tag="ACHKAU_003550"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO5532540.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            4889..5611
                     /gene="mngR"
                     /locus_tag="ACHKAU_003551"
     CDS             4889..5611
                     /gene="mngR"
                     /locus_tag="ACHKAU_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO5532541.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5715..6584)
                     /gene="sucD"
                     /locus_tag="ACHKAU_003552"
     CDS             complement(5715..6584)
                     /gene="sucD"
                     /locus_tag="ACHKAU_003552"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5532542.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6584..7750)
                     /gene="sucC"
                     /locus_tag="ACHKAU_003553"
     CDS             complement(6584..7750)
                     /gene="sucC"
                     /locus_tag="ACHKAU_003553"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5532543.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7844..9061)
                     /gene="odhB"
                     /locus_tag="ACHKAU_003554"
     CDS             complement(7844..9061)
                     /gene="odhB"
                     /locus_tag="ACHKAU_003554"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO5532544.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(9076..11877)
                     /gene="sucA"
                     /locus_tag="ACHKAU_003555"
     CDS             complement(9076..11877)
                     /gene="sucA"
                     /locus_tag="ACHKAU_003555"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5532545.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12178..12894)
                     /gene="sdhB"
                     /locus_tag="ACHKAU_003556"
     CDS             complement(12178..12894)
                     /gene="sdhB"
                     /locus_tag="ACHKAU_003556"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO5532546.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12910..14676)
                     /gene="sdhA"
                     /locus_tag="ACHKAU_003557"
     CDS             complement(12910..14676)
                     /gene="sdhA"
                     /locus_tag="ACHKAU_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO5532547.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14676..15023)
                     /gene="sdhD"
                     /locus_tag="ACHKAU_003558"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14676..15023)
                     /gene="sdhD"
                     /locus_tag="ACHKAU_003558"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO5532548.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15017..15421)
                     /gene="sdhC"
                     /locus_tag="ACHKAU_003559"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15017..15421)
                     /gene="sdhC"
                     /locus_tag="ACHKAU_003559"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5532549.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15802..15909
                     /locus_tag="ACHKAU_003560"
     CDS             15802..15909
                     /locus_tag="ACHKAU_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532550.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16115..17398
                     /gene="gltA"
                     /locus_tag="ACHKAU_003561"
     CDS             16115..17398
                     /gene="gltA"
                     /locus_tag="ACHKAU_003561"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO5532551.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4171 a         4778 c         4368 g         4262 t
ORIGIN      
        1 ctacgcttgc cagtgtctac atcatctctg caatatattg aatttgcctg cttttgtaga
       61 ccgaataaag cgttcatgcc gtatccggca tgaacaaagc gccctttgtc aacaatctgg
      121 ccgcgcatat gtggcttcgg tttgattgct attcaggcaa gccggtaact gaacgtccgt
      181 gactggccgg gtaaaaaagg ccccgataga tttgaacctt gattttcctc ggtggtaatg
      241 tgttcatcca tcatcgtttc tgagcaagaa atcacctcgc gactgaaagc aacagtcgca
      301 tcacaggttg ctgattcagc cggattaaac agccgtaaaa tcacttcttg tcggtcttca
      361 gctttcttaa gtgcgcttat caggcatccc actgggggca ttttcaacaa actataactt
      421 tccggcacgt tgaatccggc tttgttgagc ttcatcgcat cccatgggat tttgttgtaa
      481 cactgtactg gagtcagcca tgctcgcgcc tgctgagcta caccagcggc ggttggcgta
      541 ccggtataac tcaataaact taggcgacaa gaaagcagac cacgtagttg tgagtccggg
      601 actggcattt taattcccga aggccgccca ggccttaaaa gcagatcttc tttgcccagt
      661 aagcccacgc cacgcagcaa cgtaatggca aaggttttct tctcttcacc gatgacttca
      721 aattcacgta gcccttcgct aaagacagcc atgccgttac gcccttcctg taaggcaaca
      781 tagttgagca tattccatac cggaaccggc gcttctttcc agccttcttg ctgccagttg
      841 ttcattgcac tgtcgttcac ggggcgcgtt agcgaaccaa actgcgtatc tgccagaaca
      901 ctgtcggtgt taaaaggtgt agggatcagg acgcgaaggc gatgatcgtc agcctggtta
      961 tcaaggttga tatccacatc aatacgtctg ctgttatgac tcagagtgac aaccatctcc
     1021 acccctactc tgccagtgga ttgtcttgcg ctgcgttctt gcagattacg cggcaccgcc
     1081 atttcataac ggataacagc cctgctctgc caggcttcat gaataatatc gcattgcggt
     1141 ttcgcgttcg ccgcggtaat tacccactct tcttttgcgg gtgaatagtc atattcatca
     1201 ccatcatcag agctttcttc aatttgcaat acccggtcat agcgcacacc gctgtcttta
     1261 tctaccagtt gcagagaacc atcctcattg agcgcaatct gccagaaagc attttccaga
     1321 atcccttcag cgtcactttt tgccgcaatc acgttgccag gctgattcgc ttcaatataa
     1381 agcgtgcgat agcccataga agggacaatc tggttgatct ggatgtcgaa ctccataaag
     1441 ggatcataat tgccataatg aacaatttgc cgatcgatta ggcctggatc gatctcacgg
     1501 gcatggcgaa taaaatacgg cacaggctgg ccgcgatcgt cccgcaaatt aaactggctg
     1561 gcgcgcaggc gcacagtggt gttgatcact tcttcacgcg gccagggcat caggttaaac
     1621 aggacgagtt tgtcggcgtc gctctgcggc atgttgtcgg caattttgcg catgtagaaa
     1681 cgaatcagat tatccgccat gtcttcagcc agttcgaagc gggcgacgat ttcgcgatga
     1741 actttgtcac tgcaacaaca accgatactg tcgtgggcat gattttttaa gatctctttc
     1801 cacattttct ccagcaagcc gtggtggtat tcaaaaccca acgtccaggc cagtgttgcc
     1861 agcggttcca gcagattaac aatcttattt tcaatacgcg cgtgggcaat tttgatatcc
     1921 atacgcgtag aaccgatggt gcgatgcacg cgcatatatt tgccatcaat aaattcccct
     1981 ttcagggttg ccagattatc tcgctgcgct tcgatcttct caaatacctc ttcaaagcgg
     2041 ctcatcacaa acttacgttg agggtagatc tcacgtagct tatccatcac ttcgaagata
     2101 ttttgctgca atggcatctg gtcatgacca ttcggcaata aaatttcttt ggttactgac
     2161 gctttttcca gcacatcaaa ataactgtcg aggcgcttgc gtaatccgtc ttcgtcggca
     2221 ggtaagtact taccgatggc gtagcccagc ggcaacactt gcgccgtcac ttcgctaccg
     2281 tcactgcttt gccacaaaaa ctcggtttta tcagtaccgt ggcgctcaga acatccacgc
     2341 cagaacatgg tgcgggtaat gccaaatcca ttgtagatat gcggaagctg cccggacatg
     2401 ccaaaggaat ccggtaaata acctattttc atcggctcgc caaacgcgag gcagtcacgc
     2461 attccgtaca tcagattacg gacgatggat tccgcagaaa caatcgtggt atcggtctgg
     2521 gtataccagg ggccgataat caacttgccg gcttctacct gtttcttcac acggtctttg
     2581 ttttccggtt tcaccgcgaa ataatcttcg aggatcgccg tttgcccgtc gagtacgtaa
     2641 tatttgtatt cgttgtcctg ttccagtcgg cacaggatct cttccatatt attgaccagc
     2701 agaatgcgtg actcttcggt ggtgaaatac cactcccgat cccagtgcat atgcggggtg
     2761 atgtgaacgc gagataccgc tttcatcgtc gtttcctttt tgtttatggc attacgccat
     2821 cagtcagata gttgccatgc ttaaccgcgt gacgccgcca catcaggaga attgcagtcg
     2881 agattgcagc ccccaccaat gccgcaccaa accagccaat tgccgccata acaccgcccg
     2941 cgccattatc atgaagtaaa aagagcgaga aaatgccggc accgggtgtc gaaaggccga
     3001 tattcatcgc accgacaata gcgcccgtta ccatagagcc cagcacaaac gaaccaataa
     3061 cccgcagcgg atcttcaatc gccatcggga tcgccccttc ggtaatacct gccagcccta
     3121 acagccaggt ggatttcccg gtctcaattt caaactcttt aaacaggcgc ggtgcgagca
     3181 tcgtggaagc ggttacggta aatgccgaaa ccattttgac ggaggcgaaa atggcatacg
     3241 ggccgtaaac gccgttcgcc attgcgccca ggcagaatgc ataagcggct ttattcactg
     3301 gccctccaag gtcaaaggaa cacataaaac cgagaatggc acccagcaac agcgcgttac
     3361 ttcctgacag accgttcagc caggcggtaa gcgagttatt gatccatgcg acaggttctc
     3421 ccaccacaaa cagcatcaga ctgcccgctc ccaacgtacc gagcaccggg tagagataaa
     3481 aagtcaggaa gccattgaat ttactgccaa gacgcaagtg atttttcacc cagcgcatca
     3541 agtaaccggc tatcaatccg ccaacgaccg cgccgagaaa cccggagccg atcatgttgg
     3601 cggcaagtcc agccgcaaag cctggcgcta acgccggttt atctgccaga gaataggcgg
     3661 tataggccgc gagcaccggt accatcaaaa ttccgagcag cccgccgccc agcttgcggt
     3721 acatccacag ccaggagttt tcttcattaa acagatcttg tagcccgaag atttgcgaca
     3781 gtaataccgc gaccgccagc accgtgcccc ccgcgacaat caacggtacg gcaaaagaga
     3841 ttccgctcaa cagtgcctgt ttcagctccg ttttgacact tttcaccggt tgcgtatctt
     3901 gctgtacggt acgcgtctca tcgctacgct tgagggtaag cgcttgttgg atcaacgctt
     3961 ctgcatggcg aatcggctcg gcaacaggca ctgaaagcgc gggaatgcca ttaaaacgct
     4021 cactctcctt gatggcgact tcagccgcaa aaatacaggc ggttgcacta ttgagttgat
     4081 ccgccgttaa acgcccttca atgccgttag cgccttgttt ttcaacgtaa acatttacgc
     4141 cgagtttgcg tccggctttt tccagatatt ccgcagccat ataggtgtga gcaatacccg
     4201 ccggacaggc cgttacgcag acgatagttg gcgcgttgga aaaagaggca gaaggttgcg
     4261 tgcctccctt gtcatccagc gccgagagca actcatcagg cgtcgtcgcc gactgtatac
     4321 gcgcccgaat ctcatcatcc gccaggcgcg tggtcagcgc tgtcagcagt tgcatatgcg
     4381 tagtacccgc ttcattgggg ggaatcgcca gcagcaccac taaatcaacc gcttccgggc
     4441 catcaacgcc ttcccactga agcggctcgc tgagtgtggc gaccgcaaac gccgcttctt
     4501 ttaccgcagc agttttgcca tgcggcacag ccaacccttc acctaaggcg gtcgggccaa
     4561 ggctttcacg gcgatacact tcttccagaa attgctcagt actggaaatt ttccccagag
     4621 cagcaagacg ttgagtcaac gcgtggatgg cctcttcacg gctggtaaag cgcgcattca
     4681 gacacaacgc atcgcggtgg gtcagagtcg tcagattcat catgatcctc acttgatcgt
     4741 ttttatagtc gcgccagtgt gcccgataaa acaataccat tatgtgatca atttcacttg
     4801 attgaaatta tttgtattgt atttgtatta tttacaggat gaatttatcg ggcataatgg
     4861 ctaaattcgt attaatgaga tactaaaaat gggacacaag cccttatacc ggcagattgc
     4921 cgatcgcatt cgtgaacaaa ttgcgcgtgg cgagcttaaa cctggcgatg cgttacctac
     4981 cgaatcggca ttacaaacag agtttggcgt cagccgggtt acggtgcgtc aggctttgcg
     5041 ccagttagtc gagcaacaga tcctcgaaag cattcagggc agcgggactt acgtcaaaga
     5101 agagcgggtc aattacgata tttttcagtt aaccagtttt gatgaaaaac tgtcggaccg
     5161 tcacgtcgat acacacagtg aagtcctgat atttgaagtg attccggctg acgattttct
     5221 tcagcaacag ctacaaatca ccgcgcagga tcgtgtgtgg catgtgaagc gcgtgcgcta
     5281 tcgcaagcaa aagccaatgg cgctggaaga aacctggatg ccgcttgcgt tgttcccgga
     5341 tctcacctgg caggtcatgg aaaattcaaa atatcacttt attgaagaag tgaagaaaat
     5401 ggttatcgat cgtagcgaac aggaaatcat tcctctgatg ccaaccgaag agatgagccg
     5461 tctgcttaat atcagccaga caaagccgat tcttgaaaaa gtatcgcgtg gatatttagt
     5521 tgatggtcgg gtgtttgaat atagccgcaa cgcttttaat accgatgact ataaatttac
     5581 cctgatagcg caacgaaaat catcgcgata agcacaaaaa aggccatcat acgatggcca
     5641 accatgtcga aaccgacagc ccttcgccat tcctgtagag ggaagcggcg agggctattt
     5701 cttattacag atatttattt cagaacagtt ttcagtgctt caccgatatc cgccaggctg
     5761 cgaacggttt tcacgcctgc ggcttccaga gcagcgaatt tctcatccgc agtccctttc
     5821 ccaccggcaa tgatggcacc cgcgtggccc atacgtttgc ctttcggcgc agtcacacca
     5881 gcgatgtaac ccacaactgg cttggtaaca tgctctttga tgtacgctgc cgcttcttct
     5941 tcagcgctac cgccgatctc accgatcatt acgatcgctt cggtctgcgg atctttttcg
     6001 aacatttcga gaatgtcgat aaagttagag ccagggatcg ggtcaccgcc gataccaacg
     6061 caggtcgact gaccgaaacc gtaatccgtg gtctgtttaa ccgcttcata ggtcagtgta
     6121 ccggaacggg aaacgatacc cactttaccc ggtttgtgaa tgtgacccgg ctggataccg
     6181 attttgcatt cacccggagt gataacgccc gggcagttcg ggccgatcat acgaacgccg
     6241 gcttcgtcca gtttcacttt cacagtcagc atatccagcg tcgggatgcc ttcagtgatg
     6301 gtgataatca gtttgatgcc tgcatcaata gcttccagaa tggagtcttt gcagaacggt
     6361 gctggtacgt agataacaga agcggtagcg ccagtagcag caacagcttc acgcacggtg
     6421 ttgaacaccg gcaggccgag gtgggtagta ccgcctttac ctggggttac gccgccaacc
     6481 attttagtgc cgtatgcaat ggcctgttct gagtggaaag tcccctggct accagtaaag
     6541 ccctggcaga taaccttggt gtttttatca attaaaatgg acattatttc ccctccactg
     6601 cggcaacaac ctgctgagct gcatccgtca gaccttttgc tgcaataata ttcaggccgc
     6661 tgtcagccag tttcttcgcg ccgagttcgg cgttgttacc ttccagacgt accacgaccg
     6721 gtacgttaac acccacttct gctaccgcgc cgatgatacc gtcagcgatc aggtcgcaac
     6781 gaacgatacc gccgaagatg ttaaccagaa cggctttcac tttgtcgtca gagaggatga
     6841 ttttgaacgc ttcggttaca cgttctttgg ttgcgccgcc gccaacgtca aggaagttag
     6901 ccggttcgcc gccgtgcagt ttaacgatgt ccatcgtacc catcgccaga cctgcgccgt
     6961 taaccataca accgatgtta ccgtccagcg caacgtagtt cagttcccac tgtgcagcct
     7021 gtgcttcacg cggatcttcc tgcgactggt cacgcatttc gcgcagatca ggctggcgga
     7081 acagtgcgtt gccgtcagcg cccagtttgc cgtcgaggca aatcagatcg ccctgtttgg
     7141 tgatgaccag cgggttgatt tcgatcaacg ccaggtcgcg ctccaggaaa atggtcgcca
     7201 ggcccatgaa gattttggtg aactgctgaa ccagtttacc ttccagaccc agtttgaacg
     7261 ccagctcgcg tccctgatac ggcatcgggc cagtcagcgg atcaagcgca actttgtgga
     7321 tcaggtgcgg agtttcttcc gccacttttt cgatttccac gccgccttcg gtggaggcca
     7381 taaagaccac acgacgggaa ctacggtcta caacggcacc gagatacagc tctttagcga
     7441 tatcggtcgc tgcttcaacc agaatctggt taaccggttg gccattggca tctgtttgat
     7501 acgttaccag acgcttgccg agccagtttt ctgcaaaagc acggatgtct tctttgctgt
     7561 ttacaacttt cacaccgccc gctttaccgc ggccaccagc gtgaacctga catttcacta
     7621 cccacgggcc ggcaccgatt tttgaagcgg cttcttctgc ttcgcgcgga gtagtacagg
     7681 cataacccac cggtgctggt aagccatagc gggcaaaaag ttgttttgcc tgatattcat
     7741 gtaagttcat gtgttctgtc catccttcag taatcgttat cttttaaacc gtagaccgac
     7801 accctatcca tgtcggtcta cagtgcaggt gaagcttaaa ctactacacg tccagcagca
     7861 gacgcgtcgg atcttccagc aactctttga tcgttaccag gaagcccacg gattcgcgac
     7921 catcgatcag acggtgatcg taggacagcg ccaggtacat catcggcagg atctcaacct
     7981 gaccattcac cgccatcgga cgatctttga tagcgtgcat acccagaatt gcgctctgcg
     8041 gcgggttgat gatcggcgta gacatcaggg aaccgaacac accaccgttg gtgatggtga
     8101 agttaccacc ggtcagatct tcaacggtca gcttgccgtc acggcctttg actgccagct
     8161 ctttgatttt cttctcgatg tctgccatgc cgagggtatc gacatcacgc agtaccggtg
     8221 tcaccaggcc gcgcggcgta gaaaccgcca tgctgacgtc gaaatagttg tggtaaacca
     8281 cgtcatcgcc gtcgatagac gcgttcactt ccgggtaacg tttcagggct tcaaccaccg
     8341 ctttcacgta gaaggacata aagcccagac ggatgccgtg gcgtttttca aacgcttcac
     8401 cgtactgctt acgcagatcc ataatcggct tcatgttgac ttcgttgaac gtggtcagca
     8461 tggcggtgga gtttttcgct tccagcagac gctctgccac acgcttacgc aggcgagtca
     8521 tcgggacacg tttttcacta cgtgcagcca gagccggttg cgccgccgga gcagccgctg
     8581 ccggagcaga ctctttcgcc ggggctttcg ccagatgttt ttccacatct tcacgagtca
     8641 gacgaccacc cacaccggtg cctttaatgg cgctggcgtc gagattgtgt tcagccagca
     8701 ggcgacggat cgccgggctt aacgcatcgt tgttttgctc ttccagagat gcctgctggc
     8761 gttgcgccgg agtggacgct ttctcttcag atttggcgct ggtttcttta ccggcgctgt
     8821 tgccttcacg caggcgacca aggatctgac gagacgttac cgttgtacct tcatcttcca
     8881 gaaccgcatc cagaatgccg tctgctgatg ccggtacttc cagtaccact ttgtcagttt
     8941 cgatttctac cagcacttca tcccgtacga ctgcgtcgcc gggtttttta tgccaggttg
     9001 cgacggtggc atcggctacg gattcaggca ggtcagggac cagaatatct acgctactca
     9061 ttgtgtatcc tttatttatt cgacgttcag cgcgtcatta accagatctt gttgctgttt
     9121 ctggtgaacg gacatatacc ctaccgccgg agaggcggag gccgggcggc ctgcataacg
     9181 cagagaagcc ccaaacggaa tcacttcacg gaaatgatgc tggctgcagt accatgcgcc
     9241 ctggttgagc ggctcttcct ggcaccagac aaaatccttg acgtgagcaa actgctgcaa
     9301 cacttcctgc atcgctttat gcgggaacgg gtagagttgc tcgatacgca caatggcgac
     9361 atcgtgttga ttgttcttac gacgctgttc cagcaggtcg taataaacct taccagaaca
     9421 catcactacg cgcttcacgc ccttcggatc aagctcgtcg atttcaccga tggctggcag
     9481 gaaggtgccg ttcgccagtt cttcgaggct ggaaaccgcc agcggatgac gcagcaggga
     9541 tttcggcgac atcacgacca gcggacgacg catcccgcgc agcgcctgac gacgcagcat
     9601 gtggtaaacc tgtgccgggg tagacggtac gcacacctgc atgttttgct cagcacaaag
     9661 ttgcagataa cgttccagac gcgcggagga gtgctccggc ccctgccctt cgtaaccgtg
     9721 cggcagcaac atcaccagac cacacatccg gccccatttc tgttcgccag aagagatgaa
     9781 ctggtcgata accacctgcg caccgttggc gaagtcaccg aactgcgctt cccagatggt
     9841 cagagtgcgt ggttctgcgg tggcataacc gtattcaaac gccagcactg cttcttcaga
     9901 cagtacggag tcccagacgc ggaacgcgcc ctgcccgtta tggatgtgtt gcaacggcgt
     9961 gtaagtggaa ccgttagact ggttgtggat caccgcgtgg cggtggaaga aggtaccgcg
    10021 accggagtct tcacccgaca ggcgaaccgg aatgccttcg tcaaccagcg tggcgtaagc
    10081 gaggttttcc gcaccgcccc agtcgaacag tttctcaccg gcagccatcg cctggcgatc
    10141 gccataaatc ttggcaacgc gagactgcat ttcaactgct tccggcaccg tgctgatgcg
    10201 tttcgccagc tcctgcaggc gcttcatctc aactttgttc gggtactctt cgtcccattc
    10261 gtggttgagg tacggcgacc aggtgaaaga gtgcatgttc atcggacgcc actctgccac
    10321 tacgcaatcg ccagcatcca gcgcatcgcg gtacaggtta accatctcgg tggcatcttc
    10381 cagcgtcgcc actttttcct gctccagctt gtcagcgtag attttgcgcg gtgtcggatg
    10441 ttttttgatt ttctgataca tcagcggctg ggttgcgctc ggctcgtcgg cttcgttgtg
    10501 gccgtgacgg cggtagcaca ccaggtcgat gaagacatca cgtttaaagg tgttacggaa
    10561 atcgagcgcc agacgggtca caaaggcaac ggcttccgga tcatccgcgt taacgtggaa
    10621 aatcggtgcc tgaaccatct taccgatatc agtacagtac ggtgtagaac gagcatccag
    10681 cggattagag gtggtgaaac caacctggtt gttgataacg atacgtaccg taccgccaac
    10741 ttcataacca cgcgctttcg acatgttcag agtttcctga accacgccct gcccggtcac
    10801 tgcggcgtca ccgtggatgg tgattggcag cactttgttg ctgctcggct catcaagtct
    10861 gtccagacgg gcacgaacag aaccgataac taccgggctt acaatctcaa ggtgagacgg
    10921 gttaaacgcc agcgccaggt gcaccaggcc gccatcggtc tggaagtcag acgagaagcc
    10981 catgtggtat ttcacgtcac ccgtgccgag gtgttcttta tgtttaccgg caaactcgtc
    11041 gaacaagtct tgcggttttt tacccagcac gttcaccagc acgttcagac gaccacggtg
    11101 cgccatccca agaaccactt cgcgggtgcc gctgttgcca gcgtggcgga tcatctcttt
    11161 aagcatcggg attaacgcgt caccgccttc cagcgagaag cgttttgcgc cagggaattt
    11221 tgcgccgagg taacgttcaa ggccttcagc ggcggtcagt tcgcttaaga agcgtttttt
    11281 ctcttcgcta ttgaaagtcg cgcgaccaga ctcgatacgc tgttggatcc agcgtttttc
    11341 ttcggtgctg gtaatgtgca tatactcggc accaatcggg ccacagtagg tttgcttgag
    11401 ggcttccagc agctcgccga gtttcatggt ttctttgccg ctggcaaatg aaccgacgtt
    11461 gaaggtctcc tggaagtctg cttcggtcag atcgtggaaa gacggatcca gatcggccac
    11521 tttatcttgc tgccacagtc ccagcggatc gagattcgca tgctggtgac cacggaagcg
    11581 gtatgcgtta atgagctgca ggactttaac ctgcttcaca ttggtgtcag ggtcggagat
    11641 cgttgaagag taacgtgaag cgtctttcgc caggcggcgg aaatattcac gcgtttgaga
    11701 gtggaattga tccggtttga ctcccgtacc aggtaactgc tggaacgtcg aacgccagtt
    11761 agcgtcaacc gagtcaggat cggttaagaa gtcttcatag agctgttcta tccagctctg
    11821 gtttgcgcca gagaggtaag aagagtccaa ccaggctttc aaagcgctgt tctgcatcgt
    11881 gatcccttaa gcatcttttt tatgcttact tcgccgtgga tactaccacg cacagtgatg
    11941 tgcgtgccgg ggttcacttg cgagctcttg tctatgtagt ggctcgcgaa ggaaccttta
    12001 aaaactgtct tagtgtcagg ggcagttttt aaagatttcc tgcatttctt tgttacgcct
    12061 gatgcgctgc gcttatcagg cctacgggta atgacttgta ggccggataa ggcgttcacg
    12121 ccgcatccgg cactggttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacggttta
    12181 cgcattacgt tgcaacaaca tcgacttaat atggccgatg gcgcgcgtcg ggttcagccc
    12241 cttcggacat acactgacgc agttcatgat gctgtgacag cggaatacgc tgaatgcatc
    12301 gctcaaaccg tcgaggcggc tgtcagtctc ggtatcacgg ctatcgatca ggaaacgata
    12361 tgccgctaac aagcctgccg ggccgataaa cttatcggga ttccaccaga aagacggaca
    12421 agaggttgaa caacatgcgc agagaataca ttcatacagc ccgtcgagtt tttcgcgctg
    12481 ctctggcatc tgtaaatgct cgcgagctgg cggattttgt ccattattca acaggtaagg
    12541 cttaattttg tcatattgcg catagaattg tcccatgtct accaccaaat cgcggatcac
    12601 cggtaaacct ggcagcgggc gaatcacaat cttcttgccc ggctggttga gtgccgaaat
    12661 cggggtaata caggccagac cattcttgcc gttcatgttc agaccgtcgg aaccgcacac
    12721 accttcacgg caggagcggc ggaacgacag gctgggatct ttctctttca gctggataag
    12781 cgcatccagc agcatcatgt cgcgaccttc ttccgcttcc agggtgtaat cctgcatacg
    12841 cggagcatca tcaacatccg ggttatagcg ataaattgaa aactcgagtc tcattttcct
    12901 gtctccgcat tagtaagtac gaatcttcgg cgggaatgcc gggcgcagtt tcggttccat
    12961 gttgacgctt cggcgcgtca tggattccga ctctggcaga tacagggagt ggcacagcca
    13021 gttttcatca tcacgatccg ggaagtcgaa gcggctatgt gcgccacggc tttcggtacg
    13081 gaagttggca gaaacagccg ttgcatacgc cgtttccatc aggttatcca gttccaggca
    13141 ctcaacgcgc tgggtgttga actcgctgga agtgtcatcc agacgggcat ttttcagacg
    13201 ctcgcggatc actttcaact gctcaagccc tttcgccatc gcatcacctt cacggaagac
    13261 cgagaagtta tgctgcatac attcttgcag cgctttacga atcgccaccg gatcttcacc
    13321 gttacgatta ttgttccagc ggttcaggcg atccagagac acttcaacat cagactcgct
    13381 ggcatcgcgc agtgcgccct gctcggcgat agactcttgc agatgcagac ctgccgcgcg
    13441 accaaagacc accaggtcca gcagcgagtt gccgcccaga cggttagcgc cgtgtaccga
    13501 tacacaagcg atttcaccaa cggcaaacag tcccggaaca accacatctt cgcctttctc
    13561 attcacagtc agcgcctgac cggtaacttt ggtcggaata ccgcccatca tgtagtgaca
    13621 ggttgggata accggaatcg gctctttcac cggatcgacg tgagcgaagg tacgggaaag
    13681 ctccaggata cccggcagac gggattcgag aacttcttta cccaggtgat cgagtttcag
    13741 tttcgcgtgt ggcccccacg gaccatcaca gccgcgacct tcacggattt caatcatgat
    13801 ggaacgcgcc accacatcac ggcccgccag gtctttggcg ttcggcgcat aacgctccat
    13861 aaagcgttcg ccatgtttgt tcagcagata accgccttca ccacggcaac cttcggtgac
    13921 cagtacgccc gcaccggcaa tgccggtcgg gtggaattgc cacatttcca tatcctgcac
    13981 cggtacgcct gcgcggatag ccatgccgac accgtcgcca gtgttaatgt gggcgttagt
    14041 ggtggactga taaatacgcc ctgccccgcc agtcgccagc acggtagcgc gggctttgaa
    14101 ataaaccact tcaccggttt cgatgcacag tgcggtacaa cccaccaccg cgccatcctg
    14161 gtttttcacc agatccagcg cataccactc ggagaaaatg gtggtgtggt ttttcaggtt
    14221 ctgctgataa agcgtgtgca acagtgcgtg accggtacgg tcagccgccg ccgcagtgcg
    14281 tgccgcctgc tcgccgccga agtttttcga ctgaccgcca aacggacgtt gatagatacg
    14341 accatcatca agacgcgaga acggcaggcc catgtgttcg agttccagaa tcgcttccgg
    14401 cccggtttta cacatatatt caatcgcgtc ctggtcaccg atatagtccg acccttttac
    14461 ggtgtcgtac atatgccatt cccagttatc ttcatgggta ttacccagcg caacggtaat
    14521 gccgccttgc gcagaaacgg tatgggaacg ggtcgggaag actttagaga gcagcgcaca
    14581 ggtctggccg ctctgggaaa tttgcagcgc cgcgcgcata cctgcgccac cggcaccaat
    14641 cacaactgca tcaaattctc tgactggcaa tttcatcaca caccccacac cacaacgaat
    14701 ccataaatca cgtaaaccac cagtgcaacg acaatcacca gttgcagcat caggcgcaat
    14761 gccagcggtt taacgtagtc ggtcaacacc tgccacatgc cgatccaggc atgaatcaag
    14821 atagaaaaca gcgccagcag ggtgaacact ttggtgaacg cagaggcgaa gaaaccgatc
    14881 cagacttcat atgtcagttc gccactggtg gcgaaaaaac cgaccatata aatgatgtag
    14941 agcgtcagga cgatagcggt agcgcgaacg aggatgaaat catgtacgcc attgcgtcct
    15001 aatgcggagg cgttgcttac catacgagga ctcctgcgag aagtgaaagc acgacagtaa
    15061 taacaaagga gattttggcg gagcgtttac ccgcttcgaa tgtttcttcc agatagccaa
    15121 aatccatcat catgtggcga atacctacga cgacgtgata cgccagagcg gtaaggatgc
    15181 cccacatgat aaatttgacg aagaagctgc ccataatcgc ggaagcttgc tcgaaacctt
    15241 caggggaaga gaggctggta cccagaagcc acagcaggat acccactgca ataaaggtga
    15301 tcacaccgga aacgcgatgg agaatggacg ctatcgccgt gatggggaac cggatggtct
    15361 gtaggtccag attaacaggt ctttgttttt tcacatttct tatcatgaat aacgcccaca
    15421 tgctgttctt attattccct ggggactacg ggcacagagg ttaactttct gttacctgga
    15481 gacgtcggga tttccttcct ccggtctgct tgcgggtcag acagcgtcct ttctataact
    15541 gcgcgtcatg caaaacactg cttccagatg cgaaaacgac acgttacaac gctgggtggc
    15601 tcgggattgc agggtgttcc ggagacctgg cggcagtata ggctgttcac aaaatcatta
    15661 caattaacct acatatagtt tgtcgggttt tatcctgaac agtgatccag gtcacgataa
    15721 caacatttat ttaattttta atcatctaat ttgacaatca ttcaacaaag ttgttacaaa
    15781 cattaccagg aaaagcatat aatgcgtaaa agttatgaag tcggtatttc acctaagatt
    15841 aacttatgta acagtgtgga agtattgacc aattcattcg ggacagttat tagtggtaga
    15901 caagtttaat aattcggatt gctaagtact tgattcgcca tttattcgtc atcaatggat
    15961 cctttacctg caagcgccca gagctctgta cccaggtttt cccctctttc acagagcggc
    16021 gagccaaata acaaacgggt aaagccaggt tgatgtgcga aggcaaattt aagttccggc
    16081 agtcttacgt aataaggcgc taaggagacc ttaaatggct gatacaaaag caaaactcac
    16141 cctcaacggg gacacagctg ttgaactgga tgtgctgaaa ggcacgctgg gtcaagatgt
    16201 tattgatatc cgtactctcg gttcaaaagg tgtgttcacc tttgacccag gcttcacttc
    16261 aaccgcatcc tgcgaatcta aaattacttt tattgatggt gatgaaggta ttttgctgca
    16321 ccgcggtttc ccgatcgatc agctggcgac cgattctaac tacctggaag tttgttacat
    16381 cctgctgaat ggtgaaaaac cgactcagga acagtatgac gaatttaaaa ctacggtgac
    16441 ccgtcatacc atgatccacg agcagattac ccgtctgttc cacgctttcc gtcgcgactc
    16501 acatccaatg gcagtcatgt gtggtattac cggcgcgctg gcggcgttct atcacgactc
    16561 gctggatgtt aacaatcctc gtcatcgtga aattgccgcg ttccgcctgc tgtcgaaaat
    16621 gccgaccatg gccgcgatgt gttacaagta ttccattggt cagccatttg tttatccgcg
    16681 caacgatctc tcctatgccg gtaacttcct gaatatgatg ttctccacgc cgtgcgaacc
    16741 gtatgaagtt aatccgattc tggaacgtgc tatggaccgt attctgatcc tgcacgctga
    16801 ccatgaacag aacgcctcta cctccaccgt gcgtaccgct ggctcttcgg gtgcgaaccc
    16861 gtttgcctgt atcgcagcag gtattgcttc actgtgggga cctgcgcacg gtggtgctaa
    16921 cgaagcggcg ctgaaaatgc tggaagaaat tagctccgtt aaacacattc cggaatttgt
    16981 tcgtcgtgcg aaagataaaa atgattcttt ccgcctgatg ggcttcggtc accgcgtgta
    17041 caaaaattac gacccgcgcg ccaccgtaat gcgtgaaacc tgccatgaag ttctgaaaga
    17101 gctgggcacc aaagatgacc tgctggaagt ggctatggag ctggaaaaca tcgcgctgaa
    17161 cgacccgtac tttatcgaga agaaactgta cccgaacgtc gatttctact ctggtatcat
    17221 cctgaaagcg atgggtattc cgtcttccat gttcaccgtc attttcgcaa tggcacgtac
    17281 cgttggctgg atcgcccact ggagcgaaat gcacagtgac ggtatgaaga ttgcccgtcc
    17341 gcgtcagctg tatacaggat atgaaaaacg cgactttaaa agcgatatca agcgttaatg
    17401 gttgattgct aagttgtaaa tattttaacc cgccgttcaa atggcgggtt aatttttata
    17461 tgcctaaaca caaaaaattg taaaaataaa atccactatc ggacattcat atattctaaa
    17521 aaaaaatata tcggtataaa ttataaacaa atcgatgatt tttaatcatc tatcttcct
//
LOCUS       DAZFJF010000097        16878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFJF010000097 DAZFJF010000000
VERSION     DAZFJF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.097"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(109..2100)
                     /gene="tkt"
                     /locus_tag="ACHKAU_003562"
     CDS             complement(109..2100)
                     /gene="tkt"
                     /locus_tag="ACHKAU_003562"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5532552.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2378..3136
                     /gene="loiP"
                     /locus_tag="ACHKAU_003563"
                     /gene_synonym="yggG"
     CDS             2378..3136
                     /gene="loiP"
                     /locus_tag="ACHKAU_003563"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO5532553.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3192..3935)
                     /locus_tag="ACHKAU_003564"
     CDS             complement(3192..3935)
                     /locus_tag="ACHKAU_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5532554.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3922..5031)
                     /gene="dgaE"
                     /locus_tag="ACHKAU_003565"
     CDS             complement(3922..5031)
                     /gene="dgaE"
                     /locus_tag="ACHKAU_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO5532555.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5035..5895)
                     /locus_tag="ACHKAU_003566"
     CDS             complement(5035..5895)
                     /locus_tag="ACHKAU_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO5532556.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(5892..6640)
                     /locus_tag="ACHKAU_003567"
                     /pseudo
     CDS             complement(5892..6640)
                     /locus_tag="ACHKAU_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
     gene            complement(6666..7151)
                     /locus_tag="ACHKAU_003568"
     CDS             complement(6666..7151)
                     /locus_tag="ACHKAU_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5532557.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7162..7590)
                     /locus_tag="ACHKAU_003569"
     CDS             complement(7162..7590)
                     /locus_tag="ACHKAU_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5532558.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7709..10507)
                     /gene="dagR"
                     /locus_tag="ACHKAU_003570"
     CDS             complement(7709..10507)
                     /gene="dagR"
                     /locus_tag="ACHKAU_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO5532559.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(10766..11686)
                     /gene="speB"
                     /locus_tag="ACHKAU_003571"
     CDS             complement(10766..11686)
                     /gene="speB"
                     /locus_tag="ACHKAU_003571"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO5532560.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11822..12553)
                     /locus_tag="ACHKAU_003572"
     CDS             complement(11822..12553)
                     /locus_tag="ACHKAU_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532561.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(12699..14675)
                     /gene="speA"
                     /locus_tag="ACHKAU_003573"
     CDS             complement(12699..14675)
                     /gene="speA"
                     /locus_tag="ACHKAU_003573"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO5532562.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(14684..14815)
                     /gene="yqgB"
                     /locus_tag="ACHKAU_003574"
     CDS             complement(14684..14815)
                     /gene="yqgB"
                     /locus_tag="ACHKAU_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO5532563.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(14909..15052)
                     /gene="yqgG"
                     /locus_tag="ACHKAU_003575"
     CDS             complement(14909..15052)
                     /gene="yqgG"
                     /locus_tag="ACHKAU_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO5532564.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(15163..15414)
                     /gene="yqgD"
                     /locus_tag="ACHKAU_003576"
     CDS             complement(15163..15414)
                     /gene="yqgD"
                     /locus_tag="ACHKAU_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO5532565.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            15470..16624
                     /gene="metK"
                     /locus_tag="ACHKAU_003577"
     CDS             15470..16624
                     /gene="metK"
                     /locus_tag="ACHKAU_003577"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO5532566.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         4128 a         4665 c         4216 g         3869 t
ORIGIN      
        1 ataaatattt gtgatctaca acacgcttta tctattgctt gtccgcaaac ggacatatca
       61 aggtaataaa aaaggtcgcc gaagcgacct ttttacccga aatgctaatt acagcagttc
      121 ttttgctttc gcaacaacgt tatcaacagt gaagccgaac tcttcaaaca gcagctctgc
      181 cggagcagat tcaccgaagg tggtcatacc gacgatagca ccgttcaggc caacatactt
      241 gtaccagtag tcagcaatac ccgcttctac agcaacgcgt gcagtaaccg ctttcggcag
      301 tacggattca cggtaagcag catcctgctt gtcaaatgcg tcggtagacg gcatggacac
      361 cacgcgcgct ttcacgcctt cggcagtcag tttttcgtag gcagcaacag ccagttcaac
      421 ttctgaaccg gtagcgatga aaatcagttc cggctgaccg gcgcagtctt tcagcacata
      481 accaccgcgc gcgatgtttg ccagttgctc ttcagttcgt tcctgctgcg ccaggttctg
      541 acgggagagg atcagtgcgg tcgggccgtc ctgacgctca acaccgtatt tccacgcgac
      601 cgcggattca acctggtcac acggacgcca tgtagacatg ttcggggtta cgcgcagaga
      661 agcgacctgc tctaccggct ggtgagtcgg gccatcttcg cccagaccga tggagtcgtg
      721 ggtgtaaacc atcacctgac gctgtttcat cagcgcagcc atacgtacgg cgttacgtgc
      781 gtattccacg aacatcagga aggtggaggt gtacggcagg aaaccaccgt gcagggagat
      841 accgttagca atcgcggtca taccgaactc gcgaacaccg tagtggatgt agttacccgc
      901 agcatcttcg ttgattgctt tagaaccaga ccacagggtc aggttagacg gtgccaggtc
      961 agcagaaccg ccgaggaatt ccggcaacag cggaccgaaa gcttcgatag cattctgaga
     1021 cgctttacgg ctggcgattt tcgccgggtt agcctgcagt ttagcaatga actcttttgc
     1081 tttcgcgtcg aagtcagacg gcatttcgcc tttcatacgg cgggtaaatt cagcggcttc
     1141 ctgcggataa gctttcgcgt aagcagcgaa tttctcgttc catgcggatt ctttcgcctg
     1201 gcctgcttct ttcgcatccc actgagcata gatttcagac gggatttcga acggtgcata
     1261 tttccagccc agctgttcgc gggtcagggc aatttcagcg tcgcccagcg gcgcaccgtg
     1321 ggagtcgtga gtaccggctt tgttcgggga accgaaaccg atgatggttt tgcacatcag
     1381 cagggacggt ttgtcagtca ctgcgcgcgc ttcttctact gcgcgtttga tggatgccgc
     1441 gtcatgaccg tcgatgtcgc gaataacgtg ccagccgtaa gcttcgaaac gcattgcggt
     1501 gtcgtcggtg aaccagcctt caacgtgacc atcaatagag ataccgttgt catcgtagaa
     1561 cgcaatcagt ttacccagct tcagcgtacc cgccagagag caaacttcgt gggagatgcc
     1621 ttccatcatg cagccgtcgc ccatgaaggc gtaggtgtag tggtcgacaa tgtcgtggcc
     1681 cggacggtta aactgcgccg ccagcgtttt ttctgcaatc gccataccta ctgcgttggc
     1741 aataccctgc cccagcggac cggtggtggt ttccacacca gcggtgtaac ccacttccgg
     1801 gtggcccgga gttttagagt gcagctgacg gaagtttttc agttcttcca tcggcagatc
     1861 gtaaccggtg aggtgcagca ggctgtagat cagcatggag ccgtggccgt tggacagcac
     1921 gaagcggtca cggtcagccc aggacggatt ctgcgggttg tgtttcagga aatcacgcca
     1981 caggacttcg gcaatgtcag ccatacccat aggggcaccc gggtgaccgg atttggcttt
     2041 ctgtactgcg tccatgctca gcgcacgaat agcattggca agctctttac gtgaggacat
     2101 tttgactcca gatcggatga tgaagggcac gcccttaacg acttgacgac agcgcgtttt
     2161 gggctacgcc ggaaaatttg ccaacaattt accgcaagcc gcgcgtcatg tacatggaac
     2221 atccttttgc cgcttcagaa atctctggat catgctcgca tgttgcgcaa tctactcgcc
     2281 cgtccgctgc gcttttcctt atactgagac tgagcgtcga ttcacctgca aacggcgcat
     2341 ttttagaata atcctgacct tgtgcggaag agaaaacatg aaaattcgcg ccttattggt
     2401 agcaatgagc gtggcaacgg tactgactgg ttgccagaat atggactcca acggactgct
     2461 ctcatcagga gcggaagctt ttcaggctta cagtttgagt gatgcgcagg tgaaaaccct
     2521 gagcgatcag gcatgtcagg agatggacag caaggcgacg attgcgccag ccaatagcga
     2581 atacgctaaa cgtctgacaa ctattgccaa tgcgctaggc aacaatatca acggtcagcc
     2641 ggtgaattac aaagtgtata tggcgaagga tgtgaacgcc tttgcaatgg ctaacggctg
     2701 tatccgcgtc tatagcgggc tgatggatat gatgacggat aacgaagtcg aagcggtgat
     2761 cggtcacgaa atggggcacg tggcgttagg ccatgtgaaa aaaggaatgc aggtggcact
     2821 tggtacaaat gccgtgcgag tagctgcggc ctctgcgggc gggattgtcg gaagtttatc
     2881 tcaatcacaa cttggtgatc tgggcgagaa attagtcaat tcgcaattct cccagcgcca
     2941 ggaagcagaa gccgatgatt attcttacga tcttctgcgc caacgcggca tcagcccggc
     3001 aggtcttgcc accagctttg aaaaactggc aaaactggaa gaaggtcgcc aaagctcaat
     3061 gtttgacgac catcctgcat ccgccgaacg cgcccagcat attcgcgatc gtatgagcgc
     3121 ggatggggtt aagtaagtca gacataccga tctacaacat aattgtaggt cggtaacgcc
     3181 tgaatccccg atcagagtag tgatttcact ttatttagca aaacggctac atcatccggt
     3241 cgggtatatc ccgtatcagg gtctataatc gagctgtaaa catgtggcat aatgcgcggc
     3301 acccccgcct ccagacaagt tttcagaatc acactgaagt tatccaaatc aatcccgcca
     3361 gtcggctcaa tcagcgtcat accattgcgc gccgccgtgg tcgccagcgc gtatagctcc
     3421 ggaagcgacc tctcgccgcc catcggaaag aacttcgccg cgtgtgcgcc catatctagc
     3481 atcattcgca ccgccgtatc gcaggagacg cgggcaggcg taccctggct gctgctaacg
     3541 ccggtggaaa tcagcacttc gcccggcgtc cccgtcgggc taaccagcgc gttgacatgg
     3601 gtctgctcac cgcccgtagc cgcaagcgcg cctgccgcga aaccacaacc ggtaaaggtc
     3661 tggttaacgt gtgctggatg aatctgcgcc gcaatcatcg cagctttgta atactgcgcc
     3721 gggtcacccg cccccagacc tactgaaatg gacggtattt ccgccatcca acgtttaact
     3781 tcctggatcc cctcatccac cgaggagaac tgcgcggaaa gcacgccaat cactgcgtgg
     3841 ccttcggcgg cgtcaaagat ttcacgggca ttggcgatat ctttcgccag cacattaatt
     3901 gccacgcgat tacgatagaa attaatcttt tgcatgatct gcaatctcct tcagtcgcgc
     3961 cacgataagt gccatttccc cttccgccac cgtacgcgga tcaaggctaa aaacgccctg
     4021 gtggaggtta taccgccgtg cataaatcgc gatatcgcca ccacgcagtt gcgcttcgac
     4081 gacgcgagcg tcaactccca attcctgcgc atttactcga atacgaatac gccagattgc
     4141 acgcccggct tcatcctgct caatatcagc cgttaacccg tgaatcgcag aaatcgcttc
     4201 aaccactggc tggagctgtt ccgcggtgat gactgcctgc ccctggtgat aattctccag
     4261 cgcgtaaacc agcccaacca tgttctcctt gccgattttc atcgccctgg caatgccgtg
     4321 atgctgcgct ttgcaggctg caatccagat ttttttacca gtgataaacc cggaggtcgg
     4381 cgcgttaaag gcttttgcgc cgctgtagac caccatatcc gctccgctca ccacccaggc
     4441 acgtaaatcc tcttcggcgg cggcatcgac aatcagcggc aggttgtaca tctgcgcgac
     4501 atgtgcgaag tcctcgatgt tcaacattcc cttctgcacg cagtgatgcg acttcacata
     4561 cagcagcgcg gcggtcgcgt cggtaatggc actctccagc tgccagagag ccgccaggtt
     4621 acttgaaccc acctccacaa cgcgcccgcc acctaagcgg atcgcgctga ggatcgatgc
     4681 accgtaatcg acgttatggc cgcgcagcat caccacttca ttggccatgc cggagctatc
     4741 cggcatgagc gccacccggg ccttgtcgcc gcgtgtaatg gcggcggcga cagcgatggc
     4801 gatccctgct gaggcacagg aggtaacgta gctatcctcc gccccggtaa agcgagagac
     4861 cagctcgccg gttttgtcca ccagctggtc aatctccacg aaagaggatg ccgcgcgcgc
     4921 ggtggcctgc atcacctccg gcgcgacgct ggagacgccg agaatcgtca tcttgccgca
     4981 ggcgttaatc acctgtttca gtcccagttg ttgatagata ttctgcgtca tggcttacag
     5041 cactcccagc agcgagcaga ccacgctcaa cgccacaatc gacagcagaa tcgtggtgta
     5101 acgcggaccc tttttcacca aatagaagta gatggcgaac actgccgcca gcggcagcag
     5161 gccgggcgcg atggagtcca gaatctgctg taccaccact tcagagcctt tcagtgcgct
     5221 aatttttaaa ggtgaggtta tcttgacaaa gctcgcggat agcgcgccca tcatgataag
     5281 ccccagtacg ttagcgccgt agataagctc cttgatgcgc cctccctgca gcagactgat
     5341 aatcgaatcg cgccccagcg tatagccctt gtgcaccatg ccgtagctga ttgccagcgt
     5401 aatcgccggg tagagaatca gcggcaaaat accgcccatc gcgctgccat tggcggcgaa
     5461 cgggataaaa atagcaatca acagcggcat caccgccgcc cagatgatgg agtcgcccat
     5521 cccggccagc ggccccatca ggccggtttt aatcccggtg atagaggcat cgttaatcgc
     5581 ctcgccgcgg gtcttttgct cttccatggc gatggagatc ccctggatca ccgcgccaaa
     5641 cgtctgctct gagttgaaga agttcagatg gcgttttaac gcctccacct gctcctcctt
     5701 ttgcggatag agctttttga taatcggcgt catcgaggcg cagaaaatca ggctctgtaa
     5761 acgctcatag gagctggaaa cttccgcgcc cagccagtag ataaaccacg ctttggtgat
     5821 atccgctttg gtcagcgccc cggactcccg cgcgcgctca accagttcat gctgcattac
     5881 gtcgtcgctc attatgccgc tccttcattt ttcgccaggc ctttaatcag gaaggccacg
     5941 caggtgccga aaatcgccat cgccatgatg tccaccttga ggtacagcac cggaagaatc
     6001 cgccgataaa ccacggcagc aggctctttt taccaatcac cataatggtg atggcgaagc
     6061 ccagcgccgg gagaatcccg cccatgattt caaacgagtg ggtcagccag tgcggcatca
     6121 gcttgaggaa actttccact acgttctggc cgaagtagtt ggcggcgaac accactggga
     6181 aacgtagtac cagccccagc agcgccgggt agagaaaggc gcagcgcata atggctgaca
     6241 tattggccgt ttccgcgtgt ttatcggcca tatgcaccca ggccgcattt agcgtgcgac
     6301 gcaactggtc gaggaatacg ccgataacgc caaatggaat cgccagtgca atcgccaggt
     6361 tcgggtccat attggcctta acggcgatag gaatggcgat acaggccgcc agcgccgggt
     6421 ctgacggcac attgccgcct ggcgttgagg tcacacccag atagaccaac tgcatccccg
     6481 cgccgataat catcgccgca ggcatattgc cgagcagcag gccgacaaag accgccacca
     6541 ccactggctg taacagcatg gcggagaagg tatagccaag gcgcaaccgg gcaaaccagt
     6601 aatacagccc catcaggctt gcaaaaagta aggtatccat aatttgttat cccatgttca
     6661 gagggtcaga attttttcag gatatcgtcc agcgactgcg gtttatcttc cgggatggtc
     6721 tggaaaacag ccttaatgcc gcgacttttc aaatcgttaa gaatgccaac gtctttttca
     6781 tccagcgtta tgttctggaa caccgcttta cggtttggcc cgccgcctaa accgccaacc
     6841 tgaatatcag ccacgtcaaa gccctgctgc accgcttcct gaatcgccgc cagcgacggg
     6901 aacagcacca gcacgttgcc gtcgcccagc tggttctctt tccacgcggc cgcgaagctc
     6961 tggttgctaa agcagtccac tttgatattc ggcggcgcgg ccatcaggta gatatttttc
     7021 ataaacggat cggcgtccag ctcgtcgctg accaccgcaa tacggttggc ctgagactgc
     7081 cctacccact tcgtcaccac ctgtccgtgg ataagacggc tgtcaatgcg acataacacg
     7141 atgtttgcca tacgattttc cttactgttg taattgacga atatgggaga cgacgtccag
     7201 gcaactgttg cgcccagctt ccaccagatc ggccacgcag gcggtcagtg ggccatgttc
     7261 gcgcttgtcc agcgcctcca gcagcagcga ggcattaagc ccggagatca ccgccaccgg
     7321 ataatcggcg ctcagccgcg ccgccacgtt agaggtggtg ccgccgataa aatcggtcac
     7381 gataagcgaa ccttccggca tggttttcac cacggcttcg acgcgctggt aaaactcgcc
     7441 cagcgtatcg acgggcatca gcgcgatttc cgtcacgcct tcgatttcgc ccatcaccat
     7501 gcgcagactg ttgcagagct gcagccccca gccgccgtga gttagcagca gaatttcagg
     7561 taacggatga ataggtacag ttgtggtcaa aatagcctcc tttgctccag tgtttacatt
     7621 tcatcaggag caaaggacgt gccagaattt gtgtcacgca cagaggaaat gagattggat
     7681 ggggcgttaa gccccgtaaa ccaggggatc agctgtagag cagttcgtag atataaaaat
     7741 attctgcgtc cgataaacgg atagcgtaag cctcttcaat cggcaaaaag gaggatttga
     7801 tgacgctaaa ggcacgagcg tccagatcaa gccggttttc tagcgccatc tgtaacggtt
     7861 tgcggttaat cacgatacgc tcgaccatgc agcagcagtg gatcagaaaa cgtagtgtca
     7921 cctggcggct cggtttcagg gagagcgtgt tggttaagta gttcagaaca tcctcgatct
     7981 ctttcagaat tcgctgcggg ttgagcaccg aaatatggtt gatgatgctc tccatggtca
     8041 gcgcgctgat aaagcgcatg gcgctgcgct ccatctccag tcggcgctcg ctgcttgaga
     8101 gatccggcgt cagcaggctg agcaccagct ccggcccttg ctcggaaaac agttcctcca
     8161 gcgagataaa cggtatatcc ggcagtcccg gctggaaggt gccgacgatc cctgccagcc
     8221 gttcactggc attaagcgcc tgctgaatac gctccaggct gcgcacctcg ttatagtcga
     8281 gaatcaccat acgcgtgtcc tgcgacatca gctcgccaaa gctctcctcc agcacctttt
     8341 tgatcttttc cgccgtcccc attccggtta tacatgagat aaccagcact ttgccaccgc
     8401 tctcttgctg cggcgtgcag agccgacaag ggatattttt actctgcata agcacagcca
     8461 attgcgatag atcgccagtt tcgtagctta aatccagccc aacctccagc aggctggtca
     8521 gagtgatatt tggcaacaac agaacgtcaa tctggaacag cttgctgatg gtgctgccaa
     8581 aatggaccag cgatccgata tccaccatca gtatcagccg ctgccagcac cggtcctgaa
     8641 tcatccgggt cagggtttcc agggtgtcat gcaccgactg ctcaaatggc atatcaattg
     8701 cgtagaacag atcgcgctcc agcacccggt tcacatattg cgcctggctc gttgcggtgg
     8761 tcgcaccgtg agcgattaga atcacgccgc aatccgggct ggtgtcgatg cgctggcggt
     8821 agtggcgaca ctctttcagg aacaggcaca gccagaccac ctccgtcgcc gggcactgaa
     8881 tatgcaacaa ctcattgatt ttgcggcaca gaagcgtggc attgtcatac tcatctttac
     8941 agcgatccag aatcaggctg gatgaataga gttgaggtat aagccctcgc cgcacatagc
     9001 caatcagcgc aagaaagtgt ttacgtaacg ggttcgccag gttttctggc aatgtaaatc
     9061 ctaatacctg ctccacgtaa ccgatcagca gcgtcacacg ctcctcaatt tgatcgccat
     9121 agcgcggtgg atgcgccgca ctatggcggc tataaagacc atattcaaaa atcgaactga
     9181 gtttgttttt caggatagcc agcgtttctg cgggcggcac gttactgttg cgcagattga
     9241 catattcacg agtcaggaaa ctgtaaaaca ggtcgctctc ttctatttcc gcgctggtcg
     9301 ccaacgaatt ttttaacgcg ggcaacgtac gcgcatccac gttcagacga tctttaccgc
     9361 caaacaacgc atcgaccagc agacgctgct ctggcgtggt attaaacgac atctccgcca
     9421 gtcgtttatc taactctagc gtatcattac gctcggtcat tcccgacgcc cacgcctgcg
     9481 cgcacaagaa ctgaatatca cttttaagct ggccgatatt gccttccagg ggtttactta
     9541 acaaccaatg caagagcgtt ttatcaaggc ttatggtacg ttcaatttta cgactttcac
     9601 gctgaaagaa gcttacaatc aattcgagct gctcctcgac cgaacgctgg cgaatacccg
     9661 gtaagtcaat acatacctgg atacgccgct ggaaggtacg caatagtgcc gagttgaccg
     9721 actctgtagt ggcgcaaata aggcgcaccg aaattgaata tgttggccca cttgaaccaa
     9781 gtgggcggta ttcaccttta tcgagaatag aaaagagctt ctcttgtcct tcgtacggga
     9841 ggcgatgcac ttcatccaga agtaaataac ctccgtcagc ttgctccacc aatcccgttt
     9901 tattctcact tgccccggta aatgccccct tatggtgacc aaacagatgt gaggaaagca
     9961 actccggatt atgagcatat tcggcacagt taaagtaaac caacggaggt ggcgaaccta
    10021 tggtgtgctt gcaagcgaag caatgcatta actcagcaaa gaatgttttg ccaacaccag
    10081 atgcaccggt taacagcaca tgtagtccgt ggggataaag caccgctgcg cggccttttt
    10141 ctaccgcatc acggagacta cggtcgtatc cgataagtcc ggaaaacgga tcatcatctg
    10201 tatatttttc tttctttggc aataaactag ccactgaatg aactatgcat tcgcaatcat
    10261 ccagttttcg attaatgagc aattctagtg cgtgacgatg tagaaaaaaa acaggacgtc
    10321 cctgactttt taccaccagg ccttcattcc ataattgatt aagatcttta ctaaccgagt
    10381 tgcgtgccag cccaaggtta aaaccaatcg cctcagcagt aaatgctttc tccttcgcta
    10441 aatcagacag ttccagccca tacgtcagcc gctccagctc cttgtgaata acatcaatcc
    10501 gtctcatttc tttaccgctt aaaaaacaat agttgttcag aacatacact atttgtaaac
    10561 gtaagaatga gaactatcgg gaagaattaa tgttccttgt ggcaatctgg tttctgaata
    10621 ttcgtgtttt aaatattact tatggtctga aacaaccagg caggtgaaca gagctaagaa
    10681 aaagtagcgt agaaatataa aaacatatat cgcactgatg aatgtttccc ggcatgacta
    10741 tgcacaccgg gaaatattta gtaaattact cgcccttttt cgccgcctga atatacagca
    10801 tctccagcgc cagcgtcgcc gcagccagag cagtgatttc cgactgatcg tatgccggag
    10861 ccacttccac tacgtccatc ccaacgatgt tgagatcttt caggccgcgt accagtttaa
    10921 tggcgcgatc ggaggtcaga ccgccaatta ccggcgtacc ggttcccggc gcaaatgcag
    10981 gatccaggca gtcgatatca aaggtcaggt aaaccggcat atcacccaca atttgtttca
    11041 cctgggcgat aacgtcatcc acgctgcgat cgttcacctg gcaggcgtcc agcacggtaa
    11101 agccgttgtc tttatcaaat tcggtacgaa taccaatctg cacggaatga ttcggatcga
    11161 tcagaccttc tttcggcgcg gtatagaaca tggtgccgtg gtcaaattca caaccgttcg
    11221 cgtaggtatc ggtgtgggcg tcaaagtgta ccagcgccat tttgccgaaa tgcttcgcat
    11281 gagcacgcag cagcggcagc gtaacaaagt ggtcaccacc gaaggagagc atacgcttac
    11341 cggcagccag cagcttctcg gcgtgcgcct gcagcttctc gctcatctca cgggcatcgc
    11401 cgaaagcata taccagatca ccgcagtcca cgacgttcag acgctcacgc atgtcgaaat
    11461 tccacgggaa gcggttgtgt tcccaggcca gattcgtcga aacctgacgg atagctgccg
    11521 gaccgtggcg tccccccgca cgaccagaag tggccatatc gaacggcaca ccagtaatca
    11581 cccagtcagc atcgctgtca tacggctgga agttcatcgg caggcgtaaa aaaccaaagg
    11641 cattggaaac cagtgagtta tcgtattgat gacctaaggt gctcatggat ggactcctat
    11701 tacaaagtcg atataaaaaa acccttccgc gtcgttaggc ccgacgagga agggttggat
    11761 ttgcgaaaca caagtttgtg ctaattatcg ccttaaatgg gcgaggcatc aagcgaggtt
    11821 gctatttccg acacggtggc ggcgcatagc cattgttatc tgccggaaag gcttgtccag
    11881 agcacggcgg cagcgcgttt tgcgcctggc cttgtaattc aaaggcggca aagcctatta
    11941 caccggtatt acgcacatcg ccttgcgccg aattggctgc gtaggaatca gcaacgttgg
    12001 cgaactgaaa agccgcttcg gtatgtttat ctttgcggaa tcctttaatc gctaaagaat
    12061 ctccggcatt cacaatatag ccgttgttat tcagcgaacc ttgcttgccg ttcagcacat
    12121 ccaggccatc aacagtagca acgatctcgt agttggtgtt acgactgtaa ttacggacat
    12181 ataattgata attcattccg actctggcat ggacaatata gcgtggttca ttattggatg
    12241 aatcataaac acgcgtaata ggtagagaat taaaattacc atcgcgaata gcatattcca
    12301 gttcgttaat acgaatgcta taaacgcggt catagccgga aggaacatta gtcgaataac
    12361 taataacgat catctctgcc ggatcgcgcc aggctcgctc agcagatacg ccctgaaccg
    12421 atgagtgcac atccccaccc caggcagtgc cgacattgcc cgatgcactg ctattatact
    12481 gcgcattact ggaacaaccg gccagcagca cgattaatgc caccaacgcg ctacgtactt
    12541 tccatttttt cataaccgtt ccctctgcat ttaattaatg cagcgattgt agggagatta
    12601 tttaataaga aaagcagcga gattattttt aagaatatat aaaaagaaag cgggccgatt
    12661 tacgtaaata aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataggta
    12721 taaccgtaca aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga
    12781 tcggttttct tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca
    12841 tattgcagca tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg
    12901 tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga acaggttgtg catattgcca
    12961 aggatctcct gatatgcgcc gaccataaag aaaccgagca tcggcggatt ctctggatcg
    13021 tactccggca ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca
    13081 ccgtcagagt cacaggtaat atccagcagc actgcgcgac gttccggcac ttgatccagc
    13141 ccttccagcg gcagaaccgg gaacaactgg tcgatccccc atgcatccgg cattgactgg
    13201 aacagcgaga aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc
    13261 ggacgatgcg cacggttttg cggatccagc tgcttttgca cttcatggca catgctcaga
    13321 tagagttgtt ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata
    13381 tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt cacgcagaga acggcgagtt
    13441 cccggttcgt gcatttcctg ccaggtttcc cacatgcttt gcagcgcgcg cggcgcatct
    13501 tctgcaggcg cggtcggcac cgtgtattcg ttacgttcca cgccgatgat attagatacc
    13561 agcacggtgt gatgcgcagt caccgcacga cccgattcgg tgattaccgt cggatgcggc
    13621 agaccgtttt cttcacacgc atcaccaatc gcccagatga tgttgttggc atattcattg
    13681 aggccgtagt tcaccgaaca gtcggactgc gaacgggtgc cttcatagtc cacgcccaga
    13741 ccgccgccga cgtcgaagca ctgaatattg acgcccagct tgtgcagttc cacatagaaa
    13801 cgcgcggatt cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg
    13861 tggaagtgca gcagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaacaagt
    13921 tgcagtacct gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat
    13981 ttacccgagc cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga
    14041 cgttctgctt catccagcac aatggcgatt tctgacatct tctcaatgac caggtagacc
    14101 ttgtggccca tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg
    14161 ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca gcaccgccat caactctgct
    14221 ttcgagcctg cttccagtcc cagcggttcg cccgaatgaa tcagggactc aatcacgcgg
    14281 cggtgctggt taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat
    14341 tcccgcgcac gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg
    14401 aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg
    14461 agatcgacgc gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg
    14521 ttaacgtcat agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg
    14581 gcttcctggg agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct
    14641 gacgaaggca aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa
    14701 gtgtaaaaca gttaacgact atcgcagcct ggaactgcga taacaaccca taagcacatg
    14761 gattttccag cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat
    14821 tattaagcac ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg
    14881 tttttacaag agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag
    14941 aagcgccgag atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga
    15001 acgaatcttc accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt
    15061 atcacctcca cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg
    15121 gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt
    15181 tttatacccc acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc
    15241 aggaaaaatt tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa
    15301 tcgggtgaag aattgaccta aaatagccat ccagatgtta atccatccat accgattaac
    15361 actcagactg ccagtgtttt taacttgcag agtcgtggta ggatccgcta ccacagaaaa
    15421 tccacacaac agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca
    15481 cctttttacg tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatttc
    15541 tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac
    15601 ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc accactagcg cctgggtaga
    15661 catcgaagag atcacccgta acaccgttcg cgaaattggc tatgtacatt ccgacatggg
    15721 ctttgacgct aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa
    15781 ccagggcgtt gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt
    15841 tggctacgca actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg
    15901 tctggtacag cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga
    15961 cgcgaaaagc caggtgactt tccagtatga cgacggcaaa atcgttggta tcgatgctgt
    16021 cgtgctttcc actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat
    16081 ggaagagatc atcaagccga ttctgcccgc tgaatggctg acttctgcca ccaaattctt
    16141 catcaacccg accggtcgtt ttgttatcgg tggcccgatg ggtgactgcg gtctgactgg
    16201 tcgtaaaatt atcgttgata cctacggcgg catggcacgt cacggtggcg gagcattctc
    16261 tggtaaagat ccatcaaaag tggaccgttc cgctgcgtac gcagcacgtt atgttgcaaa
    16321 aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg
    16381 cgtggctgaa ccgacctcca tcatggtaga aactttcggt accgagaaag tgccttctga
    16441 acaactgact ctgctggtac gtgagttctt cgacctgcgc ccatacggtc tgattcagat
    16501 gctggatctg ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga
    16561 acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa
    16621 gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc tggccttttt cttttacctc
    16681 accgcggtgg ataggcattc acgccgcatc cggcaaaaag ctgcccgcac aataacatca
    16741 ttcttcctca acacgtttta tcgcaaatta tcatcactac tgaaaccgat tacaccaata
    16801 acaccagcca aatatttgta atattttcat attattattc agttttcaca gttgttacat
    16861 ttcttttcag taaagtct
//
LOCUS       DAZFJF010000098        16819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFJF010000098 DAZFJF010000000
VERSION     DAZFJF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.098"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..126)
                     /locus_tag="ACHKAU_003578"
     CDS             complement(<1..126)
                     /locus_tag="ACHKAU_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5532567.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNEN"
     gene            134..635
                     /gene="tnpA"
                     /locus_tag="ACHKAU_003579"
                     /pseudo
     CDS             134..635
                     /gene="tnpA"
                     /locus_tag="ACHKAU_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(653..1321)
                     /gene="ydcL"
                     /locus_tag="ACHKAU_003580"
     CDS             complement(653..1321)
                     /gene="ydcL"
                     /locus_tag="ACHKAU_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO5532568.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1624..2217)
                     /gene="tehB"
                     /locus_tag="ACHKAU_003581"
     CDS             complement(1624..2217)
                     /gene="tehB"
                     /locus_tag="ACHKAU_003581"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO5532569.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2214..3206)
                     /gene="tehA"
                     /locus_tag="ACHKAU_003582"
     CDS             complement(2214..3206)
                     /gene="tehA"
                     /locus_tag="ACHKAU_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO5532570.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3330..4310
                     /gene="ydcK"
                     /locus_tag="ACHKAU_003583"
     CDS             3330..4310
                     /gene="ydcK"
                     /locus_tag="ACHKAU_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO5532571.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4305..4841)
                     /gene="rimL"
                     /locus_tag="ACHKAU_003584"
     CDS             complement(4305..4841)
                     /gene="rimL"
                     /locus_tag="ACHKAU_003584"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO5532572.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSDELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4904..5128)
                     /gene="ydcH"
                     /locus_tag="ACHKAU_003585"
     CDS             complement(4904..5128)
                     /gene="ydcH"
                     /locus_tag="ACHKAU_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO5532573.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5268..6923)
                     /gene="opgD"
                     /locus_tag="ACHKAU_003586"
                     /gene_synonym="mdoD"
     CDS             complement(5268..6923)
                     /gene="opgD"
                     /locus_tag="ACHKAU_003586"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO5532574.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7148..8491)
                     /gene="ydcJ"
                     /locus_tag="ACHKAU_003587"
     CDS             complement(7148..8491)
                     /gene="ydcJ"
                     /locus_tag="ACHKAU_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5532575.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8708..9631
                     /gene="ydcI"
                     /locus_tag="ACHKAU_003588"
     CDS             8708..9631
                     /gene="ydcI"
                     /locus_tag="ACHKAU_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5532576.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9669..11309)
                     /gene="trg"
                     /locus_tag="ACHKAU_003589"
     CDS             complement(9669..11309)
                     /gene="trg"
                     /locus_tag="ACHKAU_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO5532577.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            11708..11857
                     /gene="hokB"
                     /locus_tag="ACHKAU_003590"
     CDS             11708..11857
                     /gene="hokB"
                     /locus_tag="ACHKAU_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO5532578.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(11835..11903)
                     /gene="yncP"
                     /locus_tag="ACHKAU_003591"
     CDS             complement(11835..11903)
                     /gene="yncP"
                     /locus_tag="ACHKAU_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO5532579.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(11929..12102)
                     /locus_tag="ACHKAU_003592"
     CDS             complement(11929..12102)
                     /locus_tag="ACHKAU_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532580.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12347..12877)
                     /gene="cybB"
                     /locus_tag="ACHKAU_003593"
     CDS             complement(12347..12877)
                     /gene="cybB"
                     /locus_tag="ACHKAU_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO5532581.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13066..14067
                     /gene="gap"
                     /locus_tag="ACHKAU_003594"
     CDS             13066..14067
                     /gene="gap"
                     /locus_tag="ACHKAU_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5532582.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14109..15548)
                     /gene="aldA"
                     /locus_tag="ACHKAU_003595"
     CDS             complement(14109..15548)
                     /gene="aldA"
                     /locus_tag="ACHKAU_003595"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO5532583.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15745..16545)
                     /gene="ydcF"
                     /locus_tag="ACHKAU_003596"
     CDS             complement(15745..16545)
                     /gene="ydcF"
                     /locus_tag="ACHKAU_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO5532584.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT         4370 a         4205 c         3910 g         4334 t
ORIGIN      
        1 attctcattc tgacgtgcca gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg
       61 cctcatttca cactcctgtt gaccacctgg tcttaactgg aatttaaatg cctgtaatcg
      121 tttcacaggt caaatgatac tttggtctgt gaataaagaa accgatatcc ggcgtggaag
      181 gcattgtgtt ttcctgatgc atgttcacct ggtctttgtc accagatacc gacgccagat
      241 tttttgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag
      301 ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tttgttaatt aactaacctc
      361 ccaaactggc gatatccagt ctggtaaaca gcctcaaagg cgtatcgggt aggttactgc
      421 gacgagatcg accagatatt gcagtcaggt attactacaa aggcgttttg tggagtcctg
      481 gctattttgc cagtagctgc ggaggtgcgc caatatccgt catccgccaa tacattgaac
      541 aacagcaaac acctggtcag gtggaaaacc gcaccttata tccccgccct gaaggatggg
      601 gttttacggc acaccggata aaaagcgcca tctaacgatg gcgcgtctgg aactactttt
      661 tgttaacgtc aaacatggtg gcatcggtcg ccatgtcatc aataacttgt ttaatatttt
      721 cgaaagccat tggcgtactt tcgttattca ggtctttacc ttcgccctga cgaacgactt
      781 tgataaccgg tttattagtc gctgcatcaa tcagctcacc ttcaaaatag aggcgagtat
      841 ccatggtacg gtggcctgta gccatttgcg tccccgcaac cactaatgca acaggaacca
      901 cttcatagaa ttgcagccct tctttgctgg tatctacacc ggtaatggcc ccacggaaaa
      961 tcagactacg cggcccagcg gtggtaacca gtggtttacg ctgcgctatc gcttctttca
     1021 tttcggtgtt ggtatagttc aaaattttat ccagaacttt ctgccctacc tgggtagaag
     1081 gttttggcac cggataataa gtgattgggt tccagacgat gctgtcatat ttgctttgat
     1141 caaaactcgg gtctacccaa cgtaaaacag gtttacctgt tgctgaggtt gtttctttta
     1201 aatcagagta attgtttaaa aaaccagaat atttatctgg ctgggtgatt ttagaggcac
     1261 aaccagacag agccaacaag ccgcataaag ctgcaacttt cgcaaatgat gtggtacgca
     1321 tgaatagttt cctgagatga ttatattact ttttaagtta tagcaaaaag cgccgcttcg
     1381 tgttgtggca aaaaaagtgt tggtgaaaat aaatttttca gccccttttc agacgacata
     1441 gtcaatgccg aaaaaggggc taagtcaatc atcagtagtt aaatttcagt aagttaagct
     1501 gctaaaaaac ttcgactact gtgattaatt tcacatccct tcgataaaaa atatgcttac
     1561 cgaaaagaag aacagtaaat catctcagcg tggggaattt tcacgcgcct tattcttagc
     1621 gggtcatttt ttacgtgcca gcatcgtggc gaaacgcagt ttaatacgat taccgttggc
     1681 gtcggtgcgg tgcagctcgc cgacgtcttc attgtatttc accctctccc agccttcgta
     1741 atatcgacgt aattctccct ctttgaaggc aaacgggaag ccgacggtac atggataatc
     1801 agcggtatcc atcgccgcca caatcaggtt gtaaccacca ggtttagtgc aacgttgcat
     1861 attggcaatc agccctggga tggttttagc ctcaaggaac atcagtacca cagtcgaaag
     1921 aataaaatcg tactgtctat caaatgtgag gttattcaga tcaacgactc gggtgtgtaa
     1981 attatccaga ttttcaatgg atttaatgcg ctctacattg gcgatactca tggcattttt
     2041 atcccatgcg tcaacatcat aaccattggc tgccaggtaa agactgttac gaccattgcc
     2101 acagcccaga tccagcgttt tacccggttt aaccactttc accgcttcca gtacttcaga
     2161 gtgtgtgcgg gttaattcat atttatcagt aaaatagttt tcgtcacgaa tgatcattct
     2221 ttgtcctctg ctttcattaa aacggcgcgc tcggttctga ctaacaattt tccctgcatc
     2281 agaagcgcaa aagtacggat gagcagtatt gcaataataa aattggtaaa gataaacagc
     2341 ggtaccgcca gcgtatggaa aaatccattt tcgctgccac tccccagatg caaaccggtg
     2401 gttgccagtg cagatacgcc gaacgagaaa ctccagaatg aagcattaaa tggctgggag
     2461 agataccatg gcatcagacg tagcataaac agcagttgca gcagtccata accaaaaagc
     2521 attttcgcca gcgtatcacc ctcgccgccg ttgacgctca accaggcact acaggccacc
     2581 agcgcaggag cgagctgaat gccgagtgat gtccgcagtg ccgtgggtaa ttctcccgaa
     2641 ctgcgcagac gctgcaagat cacaggttca aggcttagcc atgagaaaac gcctgcgcct
     2701 aaaaacacca gaccggcgtc ggtgtagccc aacgcaccac aggccattgc gctgataaag
     2761 ttgttggcaa ctgtcggcag atacagtcca ggcgtggtag cttcttcagg gtgagatccg
     2821 cgccataatc ccgcagtttg ccaggcggca taagccaact gaaccacgac accaaaactg
     2881 aacaggcaca ccgccagtgg gcgaaaccac ggaacaaaac caatcgccac cagcatcgtc
     2941 gttgccggaa acaaactcac aaaactgctc agcactggat ggcgaacttc cgccagcacg
     3001 ctatgcggaa agcgtatgag tcgggtaata aatgcgctag ttaataatcc ccagatgatc
     3061 atcgccagaa tcaccagccc atcccctaac cagtggctga cctgccaaac ctggctggca
     3121 tagcgccagg caaatcccat ccctatcgtc cccaacacaa taccaaagta gcctgccggc
     3181 aaattgagca ctttatcgct ctgcatcttc ttactcattt tgtttaattt ataaagtata
     3241 tttgaaatgc attttatgga attttctcga gcatagccag agccgcagaa tttgctacgg
     3301 ttatagtaag tcacctgtgg gaaaacgcta tgcgcaaata tcgtctaagt gaagaacagc
     3361 gagcctttag ttatcaggaa gatggcacta aaaaaaatgt gttattacgg cagattatcg
     3421 ccattagcga ttttaacgat gtgatagcag gaaccgctgg cggctggatc gatcgcgaaa
     3481 cggtgttagc acaggaagga aattgctgga tttacgacca gaatgctatt gcgttcggcg
     3541 gaacggtgat ttccggtaat acgcgcatca ccggcaccag cgtactgtgg ggagaggttt
     3601 acgcaacgga taatgtctgg atcgataata gtgaaatcag ccagggtgca tatataagtg
     3661 acggtgtcac cattcatgac tcactggttt gcgggcaatg tagaatattt ggtcacgcct
     3721 taattaatca acattctatg atcgtcgcag cacaaggctt aacgcctgac catcaactcc
     3781 tgctacaaat ttatgatcgc gcaagggtta gcgcctcgcg tattgtccac caggcacaaa
     3841 tttatggcga tgccgtcatc cggtatgctt ttattgaaca tcgtgccgaa gtttttgatt
     3901 ttgccagtat tgaaggtaat gaagagaaca acgtctggct atgcgattgc gctaaagtct
     3961 acggtcacgc gcaggtgaaa gctggcatag aagaagatgc cattccaacg attcattaca
     4021 gttcgcaggt ggcggaatat gccattgtgg aaggtaattg tgtgttaaaa catcatgtct
     4081 tgattggcgg aaatgccgtg gtacgtggtg gaccgattct gctcgacgag cacgttgtaa
     4141 tacaaggtga gagccgtatt acaggtgcgg tgataattga aaaccatgtg gagttaaccg
     4201 accacgccgt cgttgaagcc tttgatggcg acacggttca tgtccgtggt ccaaaagtca
     4261 ttaacggcga ggagcgcatt acgcgaacgc cgctggcagg gttattatga atcgataata
     4321 cgcgcgtata agttcacatc atcataggca tcattcagga actcagcctg tttcaggcaa
     4381 ccttcaagga taaaaccatt gcgcaaagcg acctggttgc tttgcggatt gtccacccga
     4441 catttgatca cgaagcgtct aagttcatca gactgggcgt aatgatgaat caatgcctgc
     4501 agcgcctgag aaatgatccc ctgcccctga tgagattcgt ccagccagta gcctatttca
     4561 gcggttttat tcagtggttc aatacgatta aacgagataa cgccgataag ttcatcttct
     4621 ttgaaaatca tgaacatttt ggcatagccg cgttgatgca acatcacatt accctgcacc
     4681 gtttttcgcg tgtcctcttc actctgaacg aactgcggcc agtttagcga ctgctgtaac
     4741 caggttttat ttttacagat taactgataa agaggagtga cgtgattttc tgcaacagca
     4801 tgtaattcaa gtgattcgct tacttttatc gtttcagtca tcttatctcc agtcaatcca
     4861 tgcagttgtt aaaaaagccg cctggctggc ggcttaggaa agtttacacc tctttgacgc
     4921 tctcctgctg caggattttg agcatctcat cttttaactg tagcttttgt tttttcatgc
     4981 ggaccacttc cgcattgtac cctcgaccgt cggaaccttc ctttctggca atttcatgat
     5041 caagtttatt gtgtttatcg aacaaggaca taaagcgagg attttcgttt ttcagacggg
     5101 atattaaatc tcgatattct ggaaacatac gcactccctg ttagtggctg acaaggtgta
     5161 tctgaaatac acaccatcag attactcatt taacttaggg aaaaatgcga gcgggatcgt
     5221 gtttaaccag acggcacctc ccgaagaagg tgccgaagaa aaaacgatta actcatcacg
     5281 cggtcgtcaa catactgacg tttatccggc gctggcggga aatactgata cagccatgtt
     5341 tcactgatag cgtcgccctg acaacgcaga tacatccgca tatcgaccgg atcagtggag
     5401 tccgaagtcg gataccagtc aaactgaata cgataaccat caatgggttc aatatagaga
     5461 atttcgattt gcttcgcttc cccactggaa agcgtaatca ccggctcaat gccttttggt
     5521 gcggcagctt tcagatcacc accaacgaaa tcgacggcaa aacgacgcgc ccatttttcg
     5581 ggatagtgtt cacctggtgc ccaaccttcc gggaaaccgc ccatgccggt acgcgtcgcc
     5641 ataacgcgcg ctaatgggca atgaacaggc ggttgcgcac tccagtacag acgatactgg
     5701 aatgcaaact catcacccgc ctttacagct ttttctggct gccagaagca gacaatgtta
     5761 tccagcgttt cgcccgttgt tgggatttcc atcaggccga tggtgccctt accccacttg
     5821 ttacgcggtt ccacccacag acttgggcgt ttgttatacc agcccataat gtcctgataa
     5881 tgggagaaat cacgatccag ttgcaataaa ccaaaccctt tcgggttgtt gtcggtgtaa
     5941 gcattgaact gcaatttttg cggattattc agcggacggc aaatccactc gccgttgccc
     6001 cgccacatgg acagacgatc agagtcatga atttgcggat gaatggtgtc gcacatccga
     6061 cgttcattag taccgcagct gaacatactg gtcatcggcg caatgcccag ctgtttaatg
     6121 tctttgcgcg catacaggtg attttccaca tccataatca cctgactttt ctcacaatgg
     6181 atagtgaact tataggcacc agtaatgctg gcgctatcga gcaacgcata aacggtaaag
     6241 gtagttgccc ccggttttac cgtatcaaac cagaaggcgg taaagtcggg gaactcttct
     6301 ttactgtcgg tgtaagtgtc aatcgccagg ccgcgggccg acaaaccgta ttgatatgtg
     6361 tcatcaacgg cgcggaaata actcgctccg aggaatgata ctacatcacg gcgcgccagt
     6421 tcgggggctt taaacacgcg aaaaccggca aagccgagat cgctttgccc ttctaattgt
     6481 ttggtatcaa cacctgcatc gttgtatttg aacaactccg ggcgaaagtg aatttcacgc
     6541 gccagatgtg ttgctggatc tacagaaaac atacgaacgc ggcgacggaa tcccattccc
     6601 atatggaaga actgagcgtc cagttgacgg ttctcaacgt tatgccagag cgatttttcg
     6661 gcgtcgtatt gaatactgtt ataagcctgc ggcgtcattg tcgccagcgt gtcaggtaac
     6721 ggacgaggcg caccacgcca cgctgtttgc gctaagtcgt gcgccattga ctgtagaatg
     6781 gagaagtcaa aacgctgggt ttgcccgtcg gcaatatcag aatctgccgc gaatgccgcc
     6841 tgagaaaaaa gagaagcaat gccgctggta ccgcacacgg cggccatagc cattgaacct
     6901 ttaataaatc gtctacgatc catacctgaa agtgagtcct tctggtgttg tgaatggtcc
     6961 cgcgctggtc gttttcagcg gcaagaaagt gcttatttat gaccgctcac tctagacaat
     7021 atttattaat aatccaattg ttgaactcag agaatatgca taaatcggca atttttttat
     7081 gtcgatggga tgagcctgat tcgtttgtaa aatcaggcaa atttttgcaa aggcggatgg
     7141 tcagaattta aagcaaacca cagcgacgtt tactgcgttc ttccgcttcc tgataaagct
     7201 ggaactcatc caaaaccgga caacccaacg cctgctcaaa tgcctcgcga ctggcattac
     7261 cgtgattgcg tgcctgcgtt tcattaccca gatttgactg gaagatcccc gccgcgctaa
     7321 cgggcaagaa atcttcatag gtaatgggtt gcgccgcgac ccaaccacgt tcaattaagg
     7381 gctgtggatc gtctccggga tgaatcgcct gacgatgcgc ctcacctgaa ggcgtcagac
     7441 ggtaccggaa ccatgccagc ccttgctgac gcattaaaaa ctcactgtca ggaaaagtgc
     7501 ggaaggtttc ctgtaaatgc atttggtgag tgagattatc ctgcccggtt ccagcgttac
     7561 gcagaagatc atcatacagt tgtcgccctt tcggcgttaa tgccacgcca cgctgctcaa
     7621 tttcaccaaa gcgcgcggta tgcgtgccct gtttctgccc cgcaaacaac accgtctctt
     7681 ccagtgcttt aaagctggtc tggcgtagta aaatcggtac ctcgcggcgc ggcggcccct
     7741 cgatgagaat tttgggttca attccgcatt caggcatcat cgactgcacc cggtcaatat
     7801 ccagcgtacg tggcgtcagg tggttgatat ggcatccagg aaaacagacc acatcagcaa
     7861 tcaaccgatg ttcgttgtgc aaggcgcgat aggtttcttc atctaccgtt gctgactggt
     7921 gccagcgaaa cgtttccagg gcttcctgca caaactcctg tgcctgtgtt tcgttaaaac
     7981 cgccctgctg ctcatattcc tctaacagtt gtcgacaacg tggggtgaag atatcgcgct
     8041 gacgcagaat ctccgccgct ttctggcgca aaatttcgtt ctcgataagc tcaaggcgga
     8101 gtaaggaggt aaaaacgcgg aagggattac gcgccagaga agcatcatca atgggccgaa
     8161 atgctgtcga atgtaccggc acccctgcct gcgagagatc gtaatagctc accgggtaca
     8221 tccccataat ggcaaacatc cgccgaagag tagcaagctc ttgtgcagtc ccaacgcgaa
     8281 tcgccccatg acgttcaaca ttcagtcgcg ccagctcgtc tgcatttacc attttttcgt
     8341 gcagttgagg attgttttcc agcacagcca gattcacatc agctaccagt tccagcagcg
     8401 tgccatattg cggaacttct tgctggtaca tggctgacat tgcctgcgaa aactgttccc
     8461 gaatctcatc cgccgtgatg ctgttcgcca tagtgtctgc ctccagtggt cagtaatctg
     8521 gagtgtaggt aaccgcattc actctggcgg gaagaattta caaactgtga tctcgccgcg
     8581 aaaacatcaa tattatccat tttgctgtaa cacaattgct ttaattgtta ataatatttt
     8641 gcaatcaagt tatcataatc aaacaacttc acttgtcagc gacaccgctt cgtttttaac
     8701 atcgcttatg gaaaaaaata gtctgtttag tcagcgcatc cgtttgcgcc accttcatac
     8761 attcgtagct gtcgcacaac aaggaacttt ggggcgcgcg gctgaaaccc ttaatttgag
     8821 tcaacctgcg ctctctaaga cattgaatga actggagcag ctgactggcg ctcgcttgtt
     8881 tgagcgtggt cgtcaggggg cgcaacttac cttacccggc gaacaatttt taacgcatgc
     8941 agtcagagtt cttgacgcca tcaacactgc cggacagtcg cttcatcgta aagaaggtct
     9001 taataatgat gtcgtcaggg ttggtgcact acctactgcg gcactgggga tattaccttc
     9061 ggttataggt cagtttcatc agcaacaaaa agagacgacc ttgcaagttg cgacaatgag
     9121 taaccctatg attctggcgg gtttaaaaac cggggaaatc gatatcggca ttggtcggat
     9181 gtcagatcct gaactgatga ccgggcttaa ttacgaactg ctgtttcttg aatcgttgaa
     9241 gctggttgtc cgccctaatc acccgctact tcaggagaac gtaacgctaa gccgggtgct
     9301 ggaatggccg gtcgttgtat caccagaagg cactgcgcca cgccagcatt cagatgcatt
     9361 agtgcagagc cagggctgta aaattccttc gggttgtatc gaaacgctgt ctgcttcgct
     9421 atctcgtcaa cttacggttg aatacgatta cgtgtggttt gtcccttctg gcgctgtaaa
     9481 agacgacctg cgtcatgcca cgctggtggc cctgcctgtt ccgggacatg gtgcaggcga
     9541 accgattgga atactgaccc gcgtagatgc gacgttctct tctggttgcc agttgatgat
     9601 taacgctatt cgaaaatcaa tgccgttctg aaaggtgaag ggatctgtcg atccctcctt
     9661 gaacattttc acaccgtagc gaaactaact ggttcacccg ctccgcgagg ttctgccgac
     9721 acagaatgtt tgtgcagacg gaatacatcc accgcctcag ttaatcgtgc cgcctgttct
     9781 tcaagggaca ccgccgccgc tgaggcctct tctaccagag aagcattctg ttgcgtcacc
     9841 ttatccattt cagaaatcgc ctggctaacc tgcgttatgc ctctactttg ttcatccgag
     9901 gcggcggcga tttcctgcat gatatgtgtg acactcgcga cggcatcaac aatagtgctc
     9961 atggttttcc ctgccgttgc cacctcatcc gaccccaggt caattaacct gactgattca
    10021 ctgatcaagc cttcaatctc tttcgccgct tgggcgctgc ggcttgcgag tgtccgtact
    10081 tcgctggcga caacggcaaa tccacggcct tgctcacccg ctcgcgcggc ttcaacggca
    10141 gcattcagtg ccagaatatt cgtctggaaa gcaatactgt tgatgacggc ggtgatctcg
    10201 gaaattttct tcgaacttgt agagatagcg cccatcgttt ttactacacc ggaaaccgtc
    10261 tgcccgccat cgctggcttt aatagaagcc tcttgcgcca gtttgctggc atgatgcgcg
    10321 ttatccgcat tctgtttcac cgtcgcagtg agttgctcca tgctagcggc agtttgttcg
    10381 atagccgccg cttgttcttc ggtgcgagat gacaggtccg cattgccagc tgaaatttcg
    10441 ctggtgccac gataaatctc ttccgcaccc tgtcgaacag tccctactgt catccccagt
    10501 gaatgctgca tttgctgtaa atgacgactt aagcgaccga tttcattacg acccgccggt
    10561 tcatcattca tcgtcagatc gccactggcg attttttcaa tccgttgtgc ggcatgttgc
    10621 agtggacgaa tgacgatccg acgtagcacc ataaatgtta tcagcgtcat gaccagggca
    10681 agcacaaacg cgccaatcat gaacatccca cccaggcggg tgcgctgatg ggccagttcc
    10741 gccagttggt tggctctggt gctacgtatc ttaacggctt tgttcaaaat atcggtataa
    10801 gcattatcca gcggtcggat ctgctcactt tcatgattga taatcgcttc aaacatgcca
    10861 tttttggcat atttcaacat aggttgcata cccgtgatat aagcctgaaa gcgttgattt
    10921 aattcagtgt cgagggcttc atcagcaggt gttttcaccg gtcgattctg ataagcacga
    10981 taaccttgct gcgactgttt aatctccgat tcggcttgcg caatatttcg cttcattgct
    11041 tccatttccg caatacgact tgcagccccg gcctgaatca tattgatccg ggctgaacgc
    11101 aaaaaatctg aactgttcgc taacccggtc cgaatctcaa tctccgcagt aacatcacgc
    11161 tgatcgcgat cggcctgcca gaggaaatag ccagccagag cagaacttaa tgcgaataga
    11221 actaagatac cgccaagaat gcaggcaaat aacggaacca gcctgatgtg atgcaaaaaa
    11281 cctaatcgat gtgagggagt tgtattcatt accgttgtct ctcgtccagg tttactcctg
    11341 atagaaagtc atcggcactt gacggtaatt acttatgacc aaaaacctct ttgcgtcaca
    11401 tttttacaac aattattgca gagaaaatta atgctattgg aatgaagaga gtaacgaatg
    11461 gctaaactgg ctaacccgaa tgacgaaaaa ttctggaaaa cagcacgcaa tttcgtgaag
    11521 tgattatttt cgtccctaag gaaacctcat aggatgcctg cgggtgcctg agactttctg
    11581 ttttcaggct aaccgggaat cagaaagacg aaggccccga ccgatatttc tatcaaccga
    11641 ggccaacgaa tgaacctagc aattcagatc ttagcctctt acccgccatc cggcaaggag
    11701 aaaggctatg aagcacaacc ctctggtggt gtgtctgctc attatctgca ttacgattct
    11761 gacattcaca ctcctgaccc gacaaacgct ctacgaactg cggttccggg acggtgataa
    11821 ggaggttgct gcgctcatgg cctgcacgtc caggtaaggg caagcgcggg gattttcccc
    11881 gcgcatctga ctagcagatt cattttctca tggcacccct tttacacttc agttagtgca
    11941 tgtttttttc gaggcactaa tagaaccatc attacagaca aatttgccgc ctgccgtaca
    12001 gtgtgagatc ccccctttcg aaccagaaca gggtttgcgc ccggcatcgg cataaaagga
    12061 aacaagcgtt cccataaaca aaataagtgc cagttttttc atataacctc catggtttat
    12121 cgaatcttat gttcgatttg aacgttatac gctggtttat tttgataccg ttgatgagat
    12181 caaagatcgg gtgaattgat taaaaataag ccagatggac gtatgggcaa ggattatggt
    12241 tttatttgtc atacaaataa atataatagg cgcttccgat gtagacccgt attcttcgcc
    12301 tgtaccacgg gtcggtttta gtacaggcgt tttctttaaa tatccttcag gaacgtttac
    12361 gcggcatcat gcgtagaagt gtgttgtcct tccagaaata gtggtgtgcc agtgcagccg
    12421 cagcgtgcaa cccgatgaca aaatatccca gattcgccag cgtctcatgc cacgacttta
    12481 agctatcaac ccgttcgaaa ttggcctctg aagcgtaagg catcgtcaaa ccaaacgcaa
    12541 accacgggtt gccccggtta tacatcatca ccaaaccaat cactggcagc gcaataaaca
    12601 ggagataaat caccaaatgt cccaaatgcg ccagtcccgt catcatcggt tttggcttag
    12661 gtataatcgg cggggttggg tatttcagtc ttaacaaaag acgaaccacc atcagcacga
    12721 gaattgagat accacaggaa acatgaacca tgttgatgag tggccgatcg ctacgtggga
    12781 agaaaccacg aaactccatt gcgcaatacg ctgcgataac cagcaaaaag accagccagt
    12841 gaatgctgat ttgtaacctt gagtatttat tttccataac atttcctgct ttaacataat
    12901 tttccgttaa cataacgggc ttttctcaaa atttcattaa atattgttca cccgttttca
    12961 gtacgttacg tttcactgta agaatggaga ttgcccatcc cacaatcctg gtctaagcct
    13021 ggaaaggatc aattttcatc cgaatgttcc tgacaggaga aaaccatgag taaagttggt
    13081 attaacggtt ttggtcgtat cggtcgactg gtgttgcgtc gattacttga agtcaaaagc
    13141 aacatagacg ttgtcgctat taatgatctc acttccccaa aaattctcgc ctacctgctg
    13201 aaacatgatt caaactacgg accattcctc tggagcgttg attttacgga agattcactt
    13261 atcgttgatg ggaaaagtat cgcggtttac gccgaaaaag aggctaaaaa tattccgtgg
    13321 aaagcgaaag gtgcagaaat cattgtcgaa tgtactggct tttatacctc cgccgagaaa
    13381 tcgcaggcgc atcttgatgc tggtgcgaag aaggtgttga tttccgcccc tgccggtgaa
    13441 atgaaaacta tcgtttataa cgtcaatgac gacactctgg atggcaacga caccattgtt
    13501 tccgtggcgt catgcaccac taactgtctt gcgccgatgg ccaaagcctt gcatgacagt
    13561 ttcgggatag aagtcggcac gatgacgacc attcatgcct atactggcac ccagtcactg
    13621 gtggatggcc cacgtggtaa agatttacgt gcttcacgcg cagcggcaga aaatatcatt
    13681 ccccacacta cgggggcggc aaaagccatt ggtctggtga tcccggaact gagcggcaaa
    13741 ctgaaaggtc atgcgcaacg cgtgccggtg aaaacaggtt cggtcactga gctggtgtcc
    13801 attctcggaa aaaaagtgac tgccgaagag gtgaataacg cacttaaaca agcaaccacc
    13861 aataacgagt catttggtta taccgatgaa gaaatagtct cttccgatat cattggcagc
    13921 catttcggtt cggtgtttga tgccacgcaa acggaaatta ccgccgtggg cgatttacaa
    13981 ctggtgaaaa cggtcgcctg gtacgataac gaatatggct tcgtcacgca gcttattcgc
    14041 accctcgaaa aattcgctaa actctgacgc gcacaggcgg aggaaaaaac ctccgcctct
    14101 tttactcatt acgactgtaa ataaaccacc tgggtctgca gatattcatg caggccatgt
    14161 ttaccatctg cgccgccaat accggattta cgccatccgg cgtggaagcc ttgcatagct
    14221 tcgaagtttt cacggttgat gtaagtttca ccaaacttca gccctttaat ggctttcatc
    14281 gcgacgttca gattttgggt atagattgat gaggtcaggc cgtaatcact gtcattagcc
    14341 attgagatag catcttccag cgtgtcaaat gcgaccaccg gcagaaccgg gccaaaggtt
    14401 tcctcatgca taatcgacat ttcctggcga acatccagca gcaatgtcgg cggataataa
    14461 tatcctttcc cgtctactgc tttgccaccc aacgccactc tcgccccttc ttctactgcg
    14521 cgcgccactt tttgctcgac cctttccagc gccgcggcgt taatcaacgg ccccatcgca
    14581 atgtcgttgc gttcagcggg gttaccaaat tgaaccgcct gcatcgcttc acccagccga
    14641 ttgacgaact gatcataaat gcctttctgt acataaacac gttctgcaca gttacacact
    14701 tgcccactat taatgacgcg tgaatcaacg atggctttga ctgccagttc aagatcggca
    14761 tcgtccatta cgatagctgg tgctttacca cccaattcca ggcacacttt ggtgatgttt
    14821 ttcgccgcag tcgccataat cttctcacct gcagagacgc tgcctgtcat actgaccatt
    14881 gcgacctttg ggttacccgc cagttcttgc ccaacggttt caccacgccc cagtacaagg
    14941 ttaaacacgc cgcgcggaag gcctatttca tcgacgattt tggcgaatgc aatcgcattg
    15001 tttggcgtaa attcactggg tttaatgacg atggtattac cggtcaaaag agcgggagcc
    15061 attttgcggg caatgaggaa gaacgggaag ttccacggca gaatgccggt agtcacacca
    15121 agcgcacgtt taaacaaaag aatattttct cctggacgat cgctttgaat aatctcgccc
    15181 tcgtaacgcc gtgcccactc cgccatgtaa tcgatatagt cggcagtaaa agccacttcg
    15241 acttcagcca gctgctggat cttgccccct tcttcaacaa tcagcgcact gatttcactg
    15301 gcgcgttcgc ggatgccggc ggagattttg cgcaaccaac tggcgcgttc aatagcaggc
    15361 aacgcttccc attctggttg tgcacgttct gctgcatcga ttgccttacg ggcatcctcg
    15421 gcctgaccat cgggtatgcg ggaaatgaca gcctctgcag cagggtttac cacatcaatc
    15481 catgcgtctc cacgccaggt aacaaactgt ccatcgatat acataggatg ttgaacgggt
    15541 actgacatgg gcgactcctg tgatttatat gtttgttttc ggtgaatgca ttgttaatta
    15601 acatcaatca tcagagagac ggcatcgtga aaggacaatt ctgtgaaggg cttcataaaa
    15661 acaagtgtaa aaggaataaa aggcagaaat aaaagtcagg agtcatgccc gtttgcaaac
    15721 aagtggttca caaacgggca atttttaacg taacgagcga ctttccatcg cctcgatgag
    15781 caccgcatca tgtttcagcg tttgccatgc atggatgact tctgccggaa aatcaacgtg
    15841 aacgataaaa tctcgcccgc ggggaccgta gccatcgcta tcatcgcgta aacgcggcag
    15901 ctcgccagtg agtagtgaga gataacgctc aaccggccat aatccttgta actgattaat
    15961 aaagattaca ctgtctgcgt tatttcctaa ctgaggaacg aatccgggat aacttaacca
    16021 gcgtggtgca tcgggattgt ccccagtcat acggcggaac gtcgccatcg tgcgccgctg
    16081 catggtgggg tcctgaacaa cgatagccgt atgaactcgt tctacggcct gattcaatag
    16141 cgcgatgcta aagcgtgcgt tttcaccgca gtttgttgac tggtcttcaa tccagatttt
    16201 ttcatgcgga atgtgccaga actgatgagc gatatccgcc aggatggtcg cttctgctct
    16261 gccagtggtg cggatagtgt tgtagtgcgg atgctgtgcg atggcgctat acaaaaaagt
    16321 tgtcgagtga ccgataccac cactaatcag taaaggaatt tgctgatcgc gagcaatctt
    16381 acatgccgcg tcgatagtcg gcataaccgc attgcctgca aggatcacgc aatcggcctg
    16441 atacggcacc tcaccggaga aatcatcctg cgccagccac tgtccgataa catttatggc
    16501 atctatagtt gccggcgaaa gcgtcggaaa cggggtaatg ttcatggttc ctccttatcg
    16561 ttcccttaag aatactgcgt gagataatag tgagaagcgg agcactctga aaggaggaag
    16621 tcgtatgtcc ttaaccgcca ctccgcaagc ctgacatatc ttttaaggaa atgctgtaga
    16681 taaacattat tcatcttgca gcagagaatt gtatttttct gaattcctga atttttaccc
    16741 ttaccgtttt agaattaata aaaaacccgg cgctaaatgc ttaccgggtt ttttctttat
    16801 caggcaaata gcagggtta
//
LOCUS       DAZFJF010000099        16791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFJF010000099 DAZFJF010000000
VERSION     DAZFJF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.099"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..226
                     /locus_tag="ACHKAU_003597"
     CDS             <1..226
                     /locus_tag="ACHKAU_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532585.1"
                     /translation="AYLVIPQINYTMDLNIEDSSSEELLSDNTLEKDENSPDNGFEVG
                     EYNTHEAYNSENQYFTREDYTYNYDLLNAI"
     gene            complement(621..997)
                     /gene="rnpB"
                     /locus_tag="ACHKAU_003598"
     ncRNA           complement(621..997)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKAU_003598"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1030..2175)
                     /gene="garK"
                     /locus_tag="ACHKAU_003599"
     CDS             complement(1030..2175)
                     /gene="garK"
                     /locus_tag="ACHKAU_003599"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO5532586.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2272..3162)
                     /gene="garR"
                     /locus_tag="ACHKAU_003600"
     CDS             complement(2272..3162)
                     /gene="garR"
                     /locus_tag="ACHKAU_003600"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5532587.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3192..3962)
                     /gene="garL"
                     /locus_tag="ACHKAU_003601"
     CDS             complement(3192..3962)
                     /gene="garL"
                     /locus_tag="ACHKAU_003601"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO5532588.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3978..5312)
                     /gene="garP"
                     /locus_tag="ACHKAU_003602"
     CDS             complement(3978..5312)
                     /gene="garP"
                     /locus_tag="ACHKAU_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO5532589.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5687..7258
                     /gene="garD"
                     /locus_tag="ACHKAU_003603"
     CDS             5687..7258
                     /gene="garD"
                     /locus_tag="ACHKAU_003603"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO5532590.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7407..7742
                     /gene="prlF"
                     /locus_tag="ACHKAU_003604"
     CDS             7407..7742
                     /gene="prlF"
                     /locus_tag="ACHKAU_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO5532591.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7742..8206
                     /gene="yhaV"
                     /locus_tag="ACHKAU_003605"
     CDS             7742..8206
                     /gene="yhaV"
                     /locus_tag="ACHKAU_003605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO5532592.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8261..9070)
                     /gene="agaR"
                     /locus_tag="ACHKAU_003606"
     CDS             complement(8261..9070)
                     /gene="agaR"
                     /locus_tag="ACHKAU_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO5532593.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9319..10599
                     /gene="kbaZ"
                     /locus_tag="ACHKAU_003607"
     CDS             9319..10599
                     /gene="kbaZ"
                     /locus_tag="ACHKAU_003607"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO5532594.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10622..11095
                     /gene="agaV"
                     /locus_tag="ACHKAU_003608"
     CDS             10622..11095
                     /gene="agaV"
                     /locus_tag="ACHKAU_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO5532595.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11106..11885
                     /gene="agaW"
                     /locus_tag="ACHKAU_003609"
     CDS             11106..11885
                     /gene="agaW"
                     /locus_tag="ACHKAU_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO5532596.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11875..12753
                     /gene="agaE"
                     /locus_tag="ACHKAU_003610"
     CDS             11875..12753
                     /gene="agaE"
                     /locus_tag="ACHKAU_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO5532597.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12771..13205
                     /gene="agaF"
                     /locus_tag="ACHKAU_003611"
     CDS             12771..13205
                     /gene="agaF"
                     /locus_tag="ACHKAU_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO5532598.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13202..14335
                     /gene="nagA"
                     /locus_tag="ACHKAU_003612"
     CDS             13202..14335
                     /gene="nagA"
                     /locus_tag="ACHKAU_003612"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5532599.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14686..15840
                     /locus_tag="ACHKAU_003613"
     CDS             14686..15840
                     /locus_tag="ACHKAU_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO5532600.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15853..16713
                     /gene="kbaY"
                     /locus_tag="ACHKAU_003614"
     CDS             15853..16713
                     /gene="kbaY"
                     /locus_tag="ACHKAU_003614"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO5532601.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         4064 a         4456 c         4277 g         3994 t
ORIGIN      
        1 tgcttatctg gtaattcccc aaattaatta tactatggat ttaaacatag aagactctag
       61 ctcagaagag ttactttcag ataacaccct cgagaaagac gaaaattctc cggacaatgg
      121 ttttgaggtc ggggaatata acacacatga agcatataac tcagagaatc aatattttac
      181 cagagaggac tatacgtata attacgacct tttaaatgca atatagactt aataacttct
      241 tacaaagtct attattaata ccgtagatat ttattcatct taccctgtgc accaaaagca
      301 aaaactcact gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg atgcgctacg
      361 cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg taggcaggat
      421 aaggcgttca cgccgcatcc ggcatgaata aagcgcactc aacaatctga aacccgccgg
      481 aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt ttgggtatag
      541 cgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc cggtttacgc
      601 tggcgggttt tcgttaaatc aggtgaaact gaccgataag ccgggttctg tcgtggacag
      661 tcattcatct aggccagcaa tcgctcactg gctcaagcag cctacccggg ttcagtacgg
      721 gccgtacctt atgaacccct atttggcctt gctccgggtg gagtttaccg tgccacggac
      781 tgttaccagc cgcgcggtgc gctcttaccg caccctttca cccttacctg atcccgcttg
      841 cgcgggccat cggcggtttg ctctctgttg cactggtcgt gggtttcccc cccaggcgtt
      901 acctggcacc ctgccctatg gagcccggac tttcctcccc tccgcccgtc tcccccgaag
      961 aggacgacga cgaagcggcg actgtctggt cagcttcggc gcgcagtata gagggtttgc
     1021 gcgcccttgt caccccgcgt tgcgcattcc aatcgccagt gtcgcggcga tattacgtga
     1081 agcacggcag atattgtcat aagccccgcg gaatgcttcg tccaacgtac caatgctggt
     1141 cagtacgctg aagacagcat caatgccatg ctgatgtaca acgccaacat catcggtcag
     1201 gctacccgca atgccaatca ccggtttatg gtacttcttc gccacgtttg cgacgccaat
     1261 cggtaccttg ccgtgaatac tctggctgtc aatacgccct tcaccggtga tcaccagcgt
     1321 acaatcgtga atatgctcct ccagattcag cgccgtagtg acgatttcaa taccactttt
     1381 cagttccgca ccaagaaacg ccattagcgc cgcgcccata ccacccgcag ctcctgcgcc
     1441 ggggacatct ttcacatcaa catgcagcgc ttttttaatg acctcggcat agtgagagag
     1501 gttattgtcc agctcaacaa tcatcgcttc actggctccc ttttgtgggc caaagatgcg
     1561 cgatgcgccg ttatcgccca ccagcggatt ggtgacatca caagcgacgc gaatgacgca
     1621 atcttttaag cgcggatcga ggccggaaat atcaatatca ttcagagtat taagactacc
     1681 gccgccaaaa ccaatttcat tgccgttggc gtcgcataat ttcgccccca gcgcctgtac
     1741 catgcctgcg ccgccatcat ttgtagcgct accgccaatg ccgataataa tgtttgtcgc
     1801 gccgctctcc agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg
     1861 atcgcgtttt tccgcaggta ccagctccag cccactggcc gccgccattt caataaacgc
     1921 ggttttgcca tcgccggaga tcccccaact ggcattcact ttctcgccca gcggccctgt
     1981 aacccaggcg tgacgttcag aaccctgggt ggctgcaatc atcgcttcca ccgttccttc
     2041 gccaccgtcg gcaaccggaa tagaaacgta ctgtgcatca ggaaaaattt cccgaaatcc
     2101 tttttctatc gcctgcgcaa cctcgctggc agataaactt tctttataag agtctggggc
     2161 gattacgatt ttcataccta tgcctgttac cacatgacgc cggagggcgt ttctcttatt
     2221 cggcctggat tccaggcccg gattgcaata cgccatccgg gcacgacgtc attaacgagt
     2281 aacttcgact ttcgccagtt tttcgtagta gcacgccagg gcgctatgat ccgccgttcc
     2341 taaaccatct gctcgcagtg cctgcatcat ctccataacc gcagctgtga gcggcagttg
     2401 cgcgccgacg ccgtgggaag tatccagcgc atttgccaga tccttaatat gcagatcaat
     2461 acggaagccc ggcttgaagt tgcggtccat caccatcggc gctttggcat ccagcacggt
     2521 actgcccgcc agtccaccgc gaattgcctg atacaccagg tccgggttaa cgcccgcttt
     2581 ggttgccagc gttagcgctt ctgacatcgc ggcaatattc agcgccacaa tgacctgatt
     2641 tgccagtttg gtgacgttac ctgcaccgat ttccccggta tgcaccacgg aacccgccat
     2701 cgctttcatc aaatcatagt atttgtcgaa aatagccttg tcgccgccca ccatcactga
     2761 cagcgtaccg tcgatggctt tcggttcacc gccgctcacc ggagcatcca gcatatcaat
     2821 gcctttcgct ttcagcgctt cgctgatttc acggcttgcc agcggtgcga tagaactcat
     2881 atcgatcaat accgtacctg gcttcgcgcc ttcaataatg ccattctcac ccagcgccac
     2941 ctctttcaca tgaggggagt ttggcagcat ggttatgatg acgtcgcact gttcagcgat
     3001 cgctttagcc gtagacgctg tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg
     3061 gttacggtca gcaaccacca gcgagtaacc tgctttcaga aggtttttac tcattggttt
     3121 acccataatc cccaggccaa taaaaccaac tttcatagtc atatcaatca tctctcttgt
     3181 tgcggtggtg gttatttttt aaaggtatca gccagtttct gagtggcaga gcggaagacg
     3241 ccaagatcgc tgccgacagc cacaaacgtc gcgccccatt ccagataacg acgcgcatcg
     3301 gcttcgaccg gcgcgagaat accgctgggt ttgccgtgcg cactggcacg gttaaaaatg
     3361 tgctgaattg ctttttgtac atccgggtgc gaggcattgc cgagatggcc taatgccgca
     3421 gccagatcgc tggggccgac gaagataccg tctacacctt cggtagcggc aatggcatcg
     3481 acgttatcta cgccctgctg actttctatc tggaccagaa tggtgatgtt cttgttcgac
     3541 tgagcgaaat aatccgccac ggtgccaaac atattggcgc ggtgagaaac ggagacgccg
     3601 cgaatgcctt ccggtgggta acgggttgat gccaccgcct gctctgcttc ctcttttgtt
     3661 tctacaaaag gaatcaggaa gttatagaaa ccgatatcca gaagacgctt aataattacc
     3721 ggctcgttgg tcggcactcg cactactggc gcgctggcgc tgcctttcag ggccattaac
     3781 tgcggaataa acgtggagat atcgtttggc gcatgttcgc catccagcac cagccagtca
     3841 aacccagcca aaccaagaac ttcagtgcta atcgggttag agagtgctga ccagcaacca
     3901 atttgtacct gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac atcgttattc
     3961 atcgcttata cctttgctta tttctgcaat tccatacgtt taatgtcgcc aactacgaag
     4021 aggtagcaga ccatcgccat cagcgctgaa catcccacga aaaccagtgc tgcattgaag
     4081 gagtgcagtt cacttaccag gtagccaatc accagtggag tgacaatgga ggcaacattg
     4141 ccaaagacgt taaagacgcc gccacagagg ccaacaatct ctttcggcgc ggtgtcagaa
     4201 atcaccggcc agcccagagc accaaatcct ttgccaaaaa aagccagcgc catcagcatg
     4261 accaccagcg tggtgttgtt ggtgtagtta cataagatga tggtggaagc cagcaacatt
     4321 cccagcacaa tcggtagctt acgtgccagg gtcagggata aaccgcgttt gatcagataa
     4381 tccgagaaga cacctcccag cacgccgccc gcaaaaccac acagtgccgg aatcgaggcg
     4441 accagaccca ctttcagaat cgacatgcct ttttcctgca ccagataaat cgggaaccag
     4501 gtgaggaaga accaggtgat ggtgttgata aaatattgtc cgaaaaatac gcccaacatc
     4561 atgcggttag agagcaattg cttgatgtaa tgcagtttgg gaccgcttgc tgccgcgctg
     4621 cccggctttt tgtggtccat atcgaccacc gcgccatttt cagagataaa cttcagctct
     4681 tccgcagaca tacgtgggtg atctgtcggg ttatgaatca atttgatcca cagcgccgtc
     4741 agcacaaaac caatcacccc cataacggta aagacgtgct cccagcccca ggcgaaagtc
     4801 agccagccaa gcagaggcga aaagagcgcc agcgagaaat attgcgccga gttaaagatg
     4861 gcggaggcag taccacgttc tttcgtcggg aaccaggcgg cgacaatacg ggcgttcgcc
     4921 gggaatgatg gcgcttccga gaagccgagc ataaagcgca taaagaacat ggagatccct
     4981 gcccaggcca gcgggaacat atcaacaaag ccttgcagga aagtgaatag cgaccagaaa
     5041 aagaggctat aggtataaac ttttttcgag ccaaacttat caagcagcca gccgccgggg
     5101 atttgcatca gcaagtaggc ccagccaaaa gcagaaaaga tgtaacccat cgaaaccgca
     5161 cttaactgca actcttttgc cacttcggta ccagcaatag acagcgttgc acgatcggcg
     5221 tagttaacgg cggtaacaat aaaaataatc agtaatatta aatagcgggt atgcacgcct
     5281 ttcttttttt cgtcaacggt gtccagaatc attttattta cctcgggtac ttatgctgat
     5341 ttttattatt atggggaagg tgttatttat gagtttcatt tatgccgtaa cggcaatgaa
     5401 ctcgggaatt agtataagca gcgcgagaat aataatcatt gtgcaaatgc taatttaatt
     5461 aatgctattt aaatattatt ttgagcatat gcacataagg ttgcgcgcta aagcacagat
     5521 ttgcgcttta ccttgccggg cggcactgca atccctgaaa tgattgacat tgatcacatt
     5581 tctgcgttta aactcctgac attcttattt cacccaatga agtcatttat ttttaaatga
     5641 gaccaggtcc tcattttaat aacccctggc tggagaatat agtacaatgg ccaacatcga
     5701 aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt cacgacacag ataatgtggc
     5761 aattattgtt aatgataatg gcctgaaagc aggaacgcgt tttccggatg gactggaatt
     5821 aattgaacat attccccagg ggcataaagt cgcattgctg gacattccgg ctaatggtga
     5881 aattattcgt tatggtgaag tgattggtta cgccgtgcgt gcaatcccac gcggaagctg
     5941 gctcgacgaa tcaatggttg tactaccgga agcgccgccg ttacacacgc tgccactggc
     6001 aaccaaagtc ccggccccct taccgccgct ggaaggatac acctttgagg gctatcgcaa
     6061 tgccgatggc agcgtgggca ccaaaaacct gctcggcatc accaccagcg tccactgtgt
     6121 ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc gatctgctac cgaaataccc
     6181 gaacgtcgat ggcgtggtgg ggctgaatca tttgtacggc tgtggcgtgg cgattaacgc
     6241 accggcggca gttgtgccta ttcgtaccat ccacaatatt tcgctgaacc ctaactttgg
     6301 cggcgaagta atggtgattg gtctgggttg tgaaaagttg cagcctgagc gcctgctggt
     6361 cggcacggat gatgtgcaag ctattccagt agaaagcgcc agcattgtca gtttgcagga
     6421 tgaaaagcat gtcggttttc agtccatggt cgaggatatt ttgcaggtcg ccgaacgcca
     6481 tctacaaaaa ctgaatcaac gtcagcgaga aacctgtccg gcttcagaac tggttgtcgg
     6541 catgcagtgc ggtggcagcg atgccttttc tggcgtaacg gcaaacccgg cggttggcta
     6601 tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg ttctcagaag tcacggaagt
     6661 gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac gaagaggtcg gcaaacggct
     6721 gctggaggag atggagtggt acgataacta tctcaatatg ggaaaaaccg accgcagcgc
     6781 caacccttcg ccgggcaaca agaaaggcgg tctggcaaac gtggtagaga aggcactcgg
     6841 ctccattgct aaatcgggta aaagcgcaat tgttgaagtg ctgtcgcccg gtcaacgccc
     6901 gactaaacgc ggattaattt acgccgcgac gccagccagc gattttgtct gtggcacgca
     6961 acaggtggct tcgggtatca ccgtgcaagt gtttacgacc ggtcgtggta cgccgtacgg
     7021 cctgatggcg gtacccgtca ttaaaatggc gacccgcacc gagctggcga accgctggtt
     7081 tgatttaatg gatattaacg cgggcactat cgccaccggc gaagaaacca ttgaagaggt
     7141 gggctggaag ttgttccact ttattctcga catcgccagc gggaagaaga aaaccttctc
     7201 ggatcaatgg gggctgcata accagctggc agtgtttaac ccggcaccgg tgacctgatt
     7261 tcttttggaa cgtccctcgc aaaacatggc cttagtgcca tgtttttatt gtttaaagcc
     7321 cccacgtcca ttaataatgc atttgcatta ccttagttca agcttataat ttgagcagaa
     7381 aacaggctgt aaaaggacag tgaatcatgc ccgctaatgc tcgctctcac gctgtactga
     7441 ccactgaatc aaaggtcacg atacgcggac aaacaactat ccccgcacca gtgcgtgagg
     7501 ccttaaaact gaagccaggc caggacagca ttcattacga aattctgcct ggtgggcaag
     7561 tctttatgtg ccgactggga gatgaacagg aggatcatac tatgaatgca tttttgcgtt
     7621 ttctggatgc agatatccag aacaacccgc aaaaaactcg tccattcaac attcaacaag
     7681 gaaagaaact tgtcgctggc atggacgtca acattgatga tgagattggc gacgacgaat
     7741 aatggatttt ccacaaaggg ttaatggttg ggcgctatat gctcatccct gttttcagga
     7801 aacctacgac gctttagttg ccgaagtcga gacattaaag ggaaaagatc ctgaaaatta
     7861 tcagagaaaa gccgccacaa agttattggc ggtagtccat aaagtgattg aggagcatat
     7921 cacggtcaat ccatcatcac cggcattccg tcatggcaag tcgttaggct ctgggaaaaa
     7981 taaagactgg tcacgggtaa aatttggtgc tggtcgttat cgtctcttct ttcgttatag
     8041 tgaaaaagag aaagtcatca ttctgggatg gatgaacgat gaaaacactc tgcgcaccta
     8101 cggtaaaaaa acagatgcct ataccgtatt cagcaaaatg ttaaaaagag gacatcctcc
     8161 tgccgactgg gaaaccctca cccgagaaac agaagaaacc cattgatggt gttcacaatg
     8221 cccgccgaac gtattcgtgt tgaccagcgg gcaccctttc tcactccccg accagaatca
     8281 cttcaacccc agcctttcgc agtccttcca gactatccgc aggaatgcct tcatcaacaa
     8341 tgatcatgtc gatacgttga gtatcaatga tcttatgtaa actggaacga ttgaatttac
     8401 tggaatcggt gaccacgatg atccgttcca caacttcgca catccgacgg tttaaacggg
     8461 cttcatcttc attatgtgtg ctgacgccgc gctccagatc gatcgcatct acaccaagaa
     8521 acagcatatc gaagtggtaa ttttgcagcg attgctcagc ctgatcgccg taaaaagatt
     8581 gcgactgacg gcgcaaatgc ccgccggtca tcagcagctc aacgccttcc gcttccagca
     8641 acgcattagc cacgttcata ccgttggtca tcgcaattac gtcagtgtgc ttacgcatca
     8701 gacgagcaat ctcaaaagtg gtagtcccgg aatcgaggat cacccgatga ccaggctgaa
     8761 tcaactcaac ggcagctttc gcaacgctgc gtttcatcgc ggtgttcagc gcgcttttat
     8821 cttccactga tggctcgact gacggcgtcg tgctatcgca gatcaacgcg ccaccatagg
     8881 cacgcacagc gatcccctgc ttttccagaa acgccagatc attgcggatc gtcacagtag
     8941 atacgccata caatgccgac agatcgttaa cctgcacact cccttgctgt cgcagacgct
     9001 gaatgatctg ttctcgtcgc tcgctggtgc cagtcactcg cttctcacct gaagcgtcgg
     9061 tattactcat agtaagtcct ttcgtaaaac tttcgtttca tttcgttttg cctattaacg
     9121 cctttctatt aagcaaatgc aagcccacct tgcccattga cgcaagctac tctcgtttca
     9181 gtgactttca ttatgtttct tttgtgaatc agatcagaaa actattatct ttcgttttat
     9241 ttttatctca ccatgacgca gtatcaactg aaacaaaacg aaagattaat atcgcagtaa
     9301 tctgaactgg agaggaaagt gaaacatctg acagaaatgg tgagacagca caaagcgggc
     9361 aaaacaaatg gaatttatgc cgtttgttcc gcacatccgc tggtgctgga ggctgcaatc
     9421 cgctacgcca gtgcaaacca aacgccgtta ctgattgaag caacctccaa tcaggtagac
     9481 cagttcggcg gttataccgg aatgacgccc gccgattttc gcggctttgt ttgtcagctc
     9541 gccgactcgt tgaatttccc gcaggatgcg ttgattctgg gtggtgacca tctggggcca
     9601 aaccgctggc aaaacctgcc ggccgctcag gcaatggcca atgccgatga tttgattaaa
     9661 agctacgttg cggcaggatt caaaaaaatc caccttgatt gcagcatgtc ctgtcatgac
     9721 gatccgattc ccttaactga tgacatcgtg gctgaacgcg ccgcccgtct ggcgaaagtg
     9781 gcggaagaaa cctgtcttga acactttggc gaagccgatc tggagtatgt cattggtacc
     9841 gaagtgccgg tacctggcgg cgcgcatgaa accttaagcg agctggcggt caccacgccg
     9901 gatgccgccc gcgccacgct ggaagcccat cgtcacgcct ttgaaaagca aggtttgaat
     9961 gccatctggc cacgcatcat tgccctggtg gttcaacccg gcgtcgaatt cgatcacacc
    10021 aacgttattg attatcagcc cgccaaagcg agcgccttaa gccagatggt cgaaaactac
    10081 gaaacgctga ttttcgaagc gcactctacc gattatcaaa cgccgcaatc gctgcgccag
    10141 ctggtgattg accactttgc cattctgaaa gttggcccag cgctgacctt cgccctgcgt
    10201 gaagctctgt tctctctggc ggcgattgaa gaagaactgg tgccagcgaa agcctgttct
    10261 ggtctgcgtc aggtgctgga agacgtgatg ctcgaccgcc cggaatactg gcaaagccac
    10321 taccacggtg acggcaacgc gcgtcgtctg gcgcgtggtt atagctactc ggatcgcgtg
    10381 cgctattact ggccggacag ccagattgat gacgctttcg ctcatctggt tcgtaatctg
    10441 gcggattcac caattccgct accgctcatc agccagtatc tgccgctgca atacgtgaaa
    10501 gttcgctccg gcgagctgca accgacgcca cgggaactca ttatcaacca tattcaggac
    10561 atcctggcgc agtaccacac agcctgtgaa ggccaataag caaaacaaag aggaacacgc
    10621 tatgccaaat attgttttaa gccggattga tgaacgcttg attcacggtc aggtcggcgt
    10681 tcagtgggtc ggatttgcgg gggcaaatct ggtgctggta gccaacgatg aggttgccga
    10741 agatccggta caacaaaacc tgatggaaat ggtactggca gaagggatcg ccgtacgttt
    10801 ctggacgctg caaaaagtta tcgacaacat tcatcgcgcc gccgatcgac agaaaatcct
    10861 gctggtttgt aaaacacccg ccgatttcct gacgctggtg aaaggtggcg ttccggtgaa
    10921 tcgcattaac gttggcaata tgcactacgc caatggcaaa caacaaatcg ccaaaacggt
    10981 ttctgtggat gcgggcgata tcgcagcatt taacgacctg aaagccgctg gggtggaatg
    11041 cttcgttcag ggcgtcccga cagagcctgc tgtagacctc tttaaattac tttgagggat
    11101 tcatcatgga aatcagcctg ttgcaggcat ttgcgttggg cattatcgcc tttatcgctg
    11161 gcctggatat gtttaacggc ctaacacata tgcaccgccc ggtggtcctc ggcccgttgg
    11221 tcgggctggt acttggcgat ctgcataccg gaattttaac cggcggtacg ctggaactgg
    11281 tgtggatggg gctggccccg ctggcgggcg cacagccgcc taacgtgatt atcggtacta
    11341 tcgtcggcac ggcgtttgcc attactactg gcgtgaaacc cgatgtcgcg gtcggtgtcg
    11401 ccgtaccttt cgctgtcgca gtacagatgg ggattacctt cctgttctcg gtgatgtctg
    11461 gcgtgatgtc tcgctgcgac cggatggcgg agaacgccga tactcgcggc attgaacgcg
    11521 tgaactatct ggcactgctg gcactcggca ctttctattt tctctgcgct ttcctgccta
    11581 tctacttcgg cgcggaacat gcaaaaacca tcattgatgt cctgccgcaa cgattaatcg
    11641 acggcctcgg cgtcgcgggc ggcatcatgc cagcaatcgg ttttgccgtg ctgctgaaaa
    11701 tcatgatgaa aaacgtctac atcccctact tcatcctggg ctttgttgcc gcagcctggc
    11761 tcaagttacc ggtgctggct attgctgccg ctgcgctggc gatggcgctg atcgacctgc
    11821 tgcgtaaatc tcctgaaccg actcaacctg cggcacagaa agaggaattt gaagatggca
    11881 tctaatcaaa ccaccctgcc gaacgtcagt gaaaacgaag aaacattgct gactggcgtc
    11941 aatgaaaacg tgtatgaaga tcagagcatt ggcgcggagc tgacgaaaaa agatatcaac
    12001 cgcgtcgcct ggcgttccat gctgttacaa gcgtctttta actacgaacg tatgcaggct
    12061 tctggctggt tgtacggtct gctgcctgct ctgaaaaaga tccacactaa taaacgcgac
    12121 ctggcgcgcg ccatgaaggg gcatatgggt ttcttcaata cccatccgtt tctggtgaca
    12181 tttgttatcg gcattatcct tgcgatggag cgttctaagc aggacgttaa cagtattcag
    12241 agcaccaaaa ttgccgtcgg tgcgccgctc ggcgggattg gcgatgcaat gttctggcta
    12301 acgctactgc cgatttgtgg cgggattgga gccagtctgg cattgcaagg ctccattctt
    12361 ggcgcagtcg tctttattct gctgttcaac gtggtacacc tggggctgcg ttttggtctg
    12421 gcgcattatg cttaccgcat gggcgtggcg gcgattccac tcattaaagc gaataccaaa
    12481 aaagtcggcc atgctgcgtc tatcgttggg atgacggtaa tcggtgcgct ggtggcaacc
    12541 tatgttcgtt taagcaccac gctggaaatc acagcgggcg acgcagtggt taagttacag
    12601 gctgatgtta tcgacaaact gatgcctgcc ttcttaccgt tggtctacac cctgaccatg
    12661 ttttggttgg tacgccgcgg ctggagtccg ctgcgtctga ttgcagttac cgtggttctc
    12721 ggcatcgtcg gtaaattctg ccatttcctt taaatacaat gaggtattag atgttaagta
    12781 ttattttgac agggcatggc gggtttgcca gcggcatgga aaaagcaatg aagcaaattc
    12841 tcggtgagca atcgcagttt atcgccatcg attttccgga aacctcaagc accgcgctgc
    12901 ttacatcgca gcttgaagag gccatcgccc aactggattg tgaagacggc attgtttttc
    12961 tgacggattt gctgggcggc acgccgtttc gcgtagcttc aacgctggcg ctgcaaaaac
    13021 cgggctgtga agtgatcacc ggcaccaatt tgcaactgct actggagatg gtgctggagc
    13081 gcgaagggtt aagcggtgaa gaattccgtg tacaggcgct ggagtgtgga catcgtgggc
    13141 tgaccagtct ggtggacgag ttaggccgct gccatgaaga atgtccggtc gaggaaggga
    13201 tatgacacac gttctgcgcg ccagaaggct gctgactgaa gagggatggc tcgatgacca
    13261 tcagttgcgt attgctgacg gtgtcatcgc agcaatcgaa ccgattccag tgggcgtgac
    13321 tgaacgcgat gcggaactgc tctgccccgc ttacatcgac acccatgtac acggtggtgc
    13381 gggcgttgat gtaatggatg acgcgccgga tgtactcgac aagctggcaa tgcacaaggc
    13441 acgcgaaggt gtcggcagtt ggttgccgac taccgtaacc gcgccgctta acaccattca
    13501 tgcggcgctg aaacgtattg ctcaacgttg ccaacgcggc ggacctggtg cgcaagtgct
    13561 ggggagttat ctcgaaggac cgtacttcac gccgcagaat aaaggcgcgc atccgccgga
    13621 attgtttcgc gagcttgaaa ttgccgagct ggatcaattg attgccgttt ctcagcacac
    13681 cttacgcgtg gtagcgctgg caccggaaaa agagggggca ttgcaggcca tccgccatct
    13741 taaacagcaa aatgtacgag tgatgctggg gcatagcgcg gcgacctggc aacaaactcg
    13801 cgccgcgttt gatgctggtg ccgacggcct ggtgcattgc tataacggga tgacaggttt
    13861 acatcaccgc gaaccgggaa tggttggcgc gggattaacg gacaagcgcg cctggctgga
    13921 actgatagcc gatggtcatc atgtgcatcc ggcggcgatg tcgctgtgtt gttgctgtgc
    13981 aaaagagaga atcgtgctga tcaccgacgc gatgcaggca gccgggatgc cggatggtcg
    14041 ctatacgtta tgtggcgaag aagtgcagat gcacggtggc gttgtccgta ccgcgtccgg
    14101 tgggctggcg ggcagtacgc tgtctgttga tgcggcagtg cgcaacatgg tcgagttgac
    14161 gggcgtaacg cctgcggaag ccattcatat ggcatcgctg catccggcgc gaatgctggg
    14221 tgttgatggt gttctgggat cgcttaaacc gggcaaacgc gccagcatcg ttgcgctgga
    14281 tagcgggctg catgtgcaac aaatctggat tcagagtcaa ttagcttcgt tttgatattt
    14341 tgctccttta ttgggccttc acttcccccg taaggccttt ctttttcttt cgttttgatc
    14401 tgtgcagcgg tgtcggatgc gacgctaacg cgtcttatgc gacctacggt acgccgcatc
    14461 cagcaatcgg tgaacatagc gacgtcatta cgcaattctt taatctcccc ttgtcttttc
    14521 tttcaatgat cacagatttc gttttatttc ctttctctcc attgaacttt cggtttcttt
    14581 tctatagatt ttaatcaacg aaagacatca ccaagtgaaa tgaaacgaaa ggcaagtgaa
    14641 agcgacaacg cccgacgtca agttcatcag actaaggatt gagttatgcc agaaaattac
    14701 acccctgctg ccgccgcaac cggtacatgg actgaagaag agatccgcca tcagcctcgc
    14761 gcatggatcc gttcactcac caacatcgac gcgctacgtt ctgcgctcaa taacttcctt
    14821 gaaccgttac tgcgcaaaga gaatctgcgg atcatcctga ccggagccgg aacctcggca
    14881 tttatcggtg acatcatcgc gccgtggctc gccagccata ccggtaaaaa cttcagcgcc
    14941 gtaccgacca ccgatctggt caccaatccg atggactacc tgaacccagc tcatccgctg
    15001 ctgttgatct ccttcggtcg atccggcaac agcccggaaa gcgtcgctgc cgtggaactg
    15061 gcaaatcaat ttgtaccaga atgctatcac ctgccgatca cctgcaacga agcgggcgct
    15121 ctttaccaaa acgcgatcaa cagcgataac gcgtttgccc tgctgatgcc cgcagaaacg
    15181 cacgatcgcg gctttgcgat gaccagcagc attaccacca tgatggccag ctgcctcgcg
    15241 gttttcgcac ctgagacgat caacagccaa accttccgcg acgtggcgga tcgttgccag
    15301 gcgatcctga cctcactggg cgatttcagc gaaggtgtgt ttggttacgc accgtggaaa
    15361 cggatcgttt atctcggcag cggtggctta cagggcgcag cacgcgagtc ggcgctgaaa
    15421 gtgctggaac tgactgcggg taaactggcg gccttctatg attccccgac cggattccgt
    15481 catggcccga aatcactggt cgataacgaa acgctggtgg tggtatttgt ctcaagccac
    15541 ccttacaccc gtcagtatga tcttgatctg ctggctgaac tccgccgtga caaccaggca
    15601 atgcgcgtaa tcgccatcgc cgcggaaagc accgacatcg tcgctgccgg tccacatatt
    15661 atcctgccgc cgtcacgtca ctttatcgac gttgagcagg cattttgctt cctgatgtac
    15721 gcccagacgt ttgcactgat gcagtcgctg cacatgggca atacgccgga taccccatca
    15781 gccagcggca ccgttaaccg cgtggtgcaa ggcgtaatca ttcatccgtg gcaggcataa
    15841 gaggatcgca ttatgagcat tatctccact aaatatctgt tacaggacgc ccaggccaat
    15901 ggctacgcgg tgcctgcttt taacattcat aacgccgaga cgatccaggc gatcctcgaa
    15961 gtgtgcagtg aaatgcgatc gccggtgatc ctcgccggaa cgccggggac ctttaaacat
    16021 atcgcgctgg aagagatcta cgccctgtgc agcgcctatt ccacaaccta caacatgcca
    16081 ctggcgctgc atctcgacca ccacgaatcg ctggatgata ttcgccgtaa agtccacgca
    16141 ggtgtgcgca gtgcaatgat cgacggcagc cacttcccgt ttgccgagaa cgtgaagctg
    16201 gtgaaatcgg ttgttgactt ctgccactca caggattgca gcgtggaagc agaactgggc
    16261 cgcctgggcg gtgttgaaga tgacatgagc gttgacgccg aaagtgcatt cctgaccgat
    16321 ccacaagaag ctaaacgctt tgtcgaactg actggcgtcg acagcctggc ggtagcgatt
    16381 ggtacggcgc acggcttata cagcaaaacg ccgaagattg atttccagcg gctggcggaa
    16441 attcgtgaag tggtggatgt tcctctggtg ctacatggtg ccagcgatgt tccggatgaa
    16501 tttgtccgtc gcactattga acttggcgtc acaaaagtga acgttgccac agaattaaaa
    16561 atagccttcg ctggcgcggt taaagcctgg tttgcggaaa atccgcaggg taatgatcct
    16621 cgttattata tgcgcgtcgg aatggatgcg atgaaagaag ttgtcagaaa taaaattaat
    16681 gtctgtggtt cagcgaatcg aatttcagca taatcattag ttttttatga tttatcccaa
    16741 tgtacttccc gattaatatc ggggagtgcc ttaatggaaa aggagatgac t
//
LOCUS       DAZFJF010000100        16746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFJF010000100 DAZFJF010000000
VERSION     DAZFJF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.100"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(130..2091)
                     /gene="topB"
                     /locus_tag="ACHKAU_003615"
     CDS             complement(130..2091)
                     /gene="topB"
                     /locus_tag="ACHKAU_003615"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5532602.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2096..3139)
                     /gene="selD"
                     /locus_tag="ACHKAU_003616"
     CDS             complement(2096..3139)
                     /gene="selD"
                     /locus_tag="ACHKAU_003616"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO5532603.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(3256..3807)
                     /locus_tag="ACHKAU_003617"
     CDS             complement(3256..3807)
                     /locus_tag="ACHKAU_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO5532604.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3968..5824
                     /gene="sppA"
                     /locus_tag="ACHKAU_003618"
     CDS             3968..5824
                     /gene="sppA"
                     /locus_tag="ACHKAU_003618"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO5532605.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5991..7007
                     /gene="ansA"
                     /locus_tag="ACHKAU_003619"
     CDS             5991..7007
                     /gene="ansA"
                     /locus_tag="ACHKAU_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO5532606.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7018..7659
                     /gene="pncA"
                     /locus_tag="ACHKAU_003620"
     CDS             7018..7659
                     /gene="pncA"
                     /locus_tag="ACHKAU_003620"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO5532607.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7820..8092)
                     /locus_tag="ACHKAU_003621"
     CDS             complement(7820..8092)
                     /locus_tag="ACHKAU_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO5532608.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8134..8547)
                     /gene="msrB"
                     /locus_tag="ACHKAU_003622"
     CDS             complement(8134..8547)
                     /gene="msrB"
                     /locus_tag="ACHKAU_003622"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO5532609.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8889..9884
                     /gene="gapA"
                     /locus_tag="ACHKAU_003623"
     CDS             8889..9884
                     /gene="gapA"
                     /locus_tag="ACHKAU_003623"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5532610.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9968..10852
                     /locus_tag="ACHKAU_003624"
     CDS             9968..10852
                     /locus_tag="ACHKAU_003624"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO5532611.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10903..11757)
                     /gene="yeaE"
                     /locus_tag="ACHKAU_003625"
     CDS             complement(10903..11757)
                     /gene="yeaE"
                     /locus_tag="ACHKAU_003625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO5532612.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11847..12593)
                     /gene="mipA"
                     /locus_tag="ACHKAU_003626"
     CDS             complement(11847..12593)
                     /gene="mipA"
                     /locus_tag="ACHKAU_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO5532613.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13029..14963
                     /gene="yeaG"
                     /locus_tag="ACHKAU_003627"
     CDS             13029..14963
                     /gene="yeaG"
                     /locus_tag="ACHKAU_003627"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO5532614.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15076..16359
                     /locus_tag="ACHKAU_003628"
     CDS             15076..16359
                     /locus_tag="ACHKAU_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO5532615.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            16506..>16746
                     /locus_tag="ACHKAU_003629"
     CDS             16506..>16746
                     /locus_tag="ACHKAU_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5532616.1"
                     /translation="MIQSTRISMGLFFKYCLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENT"
BASE COUNT         4143 a         4314 c         4103 g         4186 t
ORIGIN      
        1 atcacattcc ctgttattac atactttaga ggcgataatt ttatccatgc aaaaaaaata
       61 tccaacgaat tttcttgatc cgctggatat ttcagaatat gactcgaata gcacgaaaga
      121 ttcactcgct tacgctatcg ccccgcttcc gacttcatct gctggcggac tttttttcgc
      181 actacgttta cgcggtgcag cctttttctt atcagcactg ccaccactgc ccggagccac
      241 aatgccgcga aactgccgca ccggcgtacg tttggcttga tcaataagct gatatagcgt
      301 ccccaccagc ggctgcataa agtcctgata gcgacactgc ttttcgctga tttgcgtcag
      361 caccgattcc cagtgcgcgg tcatgtccgg tcgcgtcgcc atttctggca gcgaatggaa
      421 taacgctttt ccggcgtcgg tagagtggat atagcgccct tttttggtca gaaaaccacg
      481 cttgaacagc agttcaataa tcccggcacg ggttgcttct gtccccagac catcggtcgc
      541 acgaaggatc tttttcagat ctttgtcctg cacaaagcgc gcgatcccgg tcatcgccga
      601 aagcagtgtt gcatcggtaa aatggcgcgg cggctgggtt tgccgctcta ccacttcacc
      661 tttttcacac agcaactcat cgcctttcgc caccacaggc aatggcgtac cgtcgttttc
      721 ttcatcgcgc tctttgctgc ctaacagcgt gcgccagcct gcttcagcaa gaaaacgggc
      781 tttagcgaca aatttgcctt tggcaatgtc cagttcgata acacacttgc ggaacaccgc
      841 atccgggcag aactgcatca ggtattgacg ggcaatcagg ttatagacct tcgcttcgtt
      901 ctccgtcagg ttgatcgcag aactccgtgc ggtcgggatg atggcgtggt gcgcatcgac
      961 ctttttgtca tcccaacagc ggttgcgtat atctggatct accactgact gcggcaacag
     1021 atccggtgca tgaacgctga tggcattcat caccgcgtgg cgtccggcaa aatgttcttc
     1081 tggcaaatag cgacaatcgg aacgcggata agtgattagc ttgtgcgttt cgtacagctt
     1141 ctggcagata tcaagcacgt tctgcgcgct cagaccgaag cgttttgccg cttcaatctg
     1201 taacgccgaa agcgaaaacg gcagcggcgc ggattctgat tcccgtttat cgttatagct
     1261 ggtgacaata gccggttgac cactaatgcg gttaaccaca tgctccgcca gtggacgatg
     1321 taacaagcgc ccttcttcat cctggtacgg ttcacacgct tcgctcggtt gccagatagc
     1381 ggtaaaccgc tcatcggcag gtgtcacgat atgcgctttg acttcaaaga agtctttcgc
     1441 cacgaagttt tcaatctctt catcgcggcg caccaccagc ccgagcacgg gtgtctgcac
     1501 gcgtcccacg gaaagtacgc cctgataacc ggcattgcga ccgagaatgg tatacgcacg
     1561 ggtcatattg atgccgtaca gccagtcggc acgcgctcgc gccagcgcag aaacgcacag
     1621 cggtacaaac tcactgttgg aacgaagacg gtcgatcgcc cgctcaaccg cctgcgggtt
     1681 caggtcgttt atcaagcaac gctgcacctg ctggcgcttt tccggtgcca gttgcagata
     1741 gtccagcact tcatccacca gcaattgccc ttcacgatcc gggtccccgg cgtgaacgat
     1801 ttcgctggct tcatgcagga agcgtttgat gacgttaagt tgtttggtca cggagggtcg
     1861 gggctgtaat tgccactttt ccgggacaat cggcaaatcc gcaagattcc agcgcgcata
     1921 gcggctgtcg taggcgtctg gctgcgcctg ctcaagcagg tgaccgatac accaggtcac
     1981 cacctgacca ttaccgcact cgataaagcc atcgccttta cggtgcggtt tgggcaggac
     2041 atcagcaatg gcgcgcgcca gactcggttt ttcggcaata aacaaccgca ttgaattaat
     2101 ggatctcaac catggcacga ccgccgcgcg ctggcaccag ttcgccaatc gccgtcagtt
     2161 caatgccaaa ctcggcggct gtagttttaa cctcattttc tgcttccggc atgaccgcca
     2221 gcagcaaacc gccagaagtt tgcggatcgc acagcagatc gcgcacttca cgcggcattt
     2281 cacccatcag atgaccgtag ctggcaaagt tacgttcggt gccgccaggc actgcgccca
     2341 acttaatgta ctcttcaacc cccggcaatt ttgggatcgc gtcatagtca acgcgcgcct
     2401 gcacacctgc gccctgacac atttcgctca aatggcccag cagaccaaaa ccagtaacgt
     2461 cggtcatcgc ttttacgcct tcgatgttgg caaaggacgc acctgcgatg ttcatccggc
     2521 acatcacttc cgtcgccagt ccctgatgtt ctggtttcaa cagtgatttt ttctcagccg
     2581 tggtaagaac gccgatcccc agcggtttcg tcaggaacag tttgcatccg gcttgtgcgg
     2641 tactgttttt cttcacccgc tcggtcggta cgatccccgt taccgccaga ccaaaaatcg
     2701 gctccggcgc atcgatggag tgaccgccag ccagcgcaat acccgcctga cggcatgcat
     2761 agcgtccacc ttcggttact tcgcgggcaa tttctgggga aagtttgtta atcggccagc
     2821 cgaggatcgc aatcgccata atcggtttgc cccccatcgc gaagatatcg ctgatggcgt
     2881 tagtcgccgc aatgcggcca aaatcgaaag gattatcaac gatcggcata aagaagtcgg
     2941 tggtactgat aacgctggtg ccattgccca gatcgtacac cgccgcatcg tcgcgggtct
     3001 cattacccac aagcaaattc ggatcaacaa acttcgcctg ctcactgtgc aggatggttt
     3061 ccaacacttt tggggaaatt ttacagccgc aaccagctcc gtggctgtat tgggtcaaac
     3121 gaatcgagtt ctcgctcatg gacatctcct gtcaatgcaa tccgggtatg gtaaccctca
     3181 ttccgtgaag tgataagtga gagtgtctga attcctgcgc ctttgctcac aatccagaca
     3241 gtttcgcgac aattatcaga aataagttac aaacggcgtc gggtccggga cgttaatcga
     3301 cgtagatgct ttcaactgcg gcgtgccgag gtagagaaaa ccgacaattt tatcctgctc
     3361 acggcaaccg aatgcttcac gcactaccgg actttcagtt aatgcgccac tgcgccagat
     3421 gccgccaaat ccttgggcaa cggctgccat ttgcatcgcc atgaccgcgc atccggcaga
     3481 catttcctgt tcccatcgcg ggactttatg attttcttcg catttcgcca ccaccgtgat
     3541 gatgagcggt gcgcggaacg gcgcattacg ggctttgtcg atagctttgt catcactacc
     3601 ggcagcaatc gccccctgtt ccagtacggc gctgaaacgc tcgcgccctt ccccttcaat
     3661 cacaaaaaaa tgccacggtt gcatggactt atggtccggc gcacgcatac ccgcacgcag
     3721 gatgttttgc agttgttcac ccgttggcgc gggttcagcc aggcgggagg cgctacggcg
     3781 attgatcaat agttcgagtg catccatttg attaactcct gtcgtgatat ttattcacaa
     3841 aattaacacg agagtggatt ttgttacagc acagtccgca attcctgctg acaagtgccg
     3901 gttgggtcat tacgataacc acatctattg cgcctgtgac aggtgtgacc ttaagttggg
     3961 agaatacatg cgaacccttt ggcgatttat tgccggattt tttaaatgga cgtggcgtct
     4021 gctgaatttc gtccgtgaaa tggtacttaa cctgttcttt attttcctcg tactggttgg
     4081 tgtggggatc tggatgcagg tcagtggtgg tgattcgaaa gaaacggcca gtcgtggcgc
     4141 actgctgctg gacatttctg gtgtgatcgt cgataaaccc gacagttctc agcggtttag
     4201 taaattaagc cgccagctgc ttggtgccag ttccgatcgt ctgcaggaaa actcactgtt
     4261 tgatatcgtc aacaccattc gccaggcgaa ggacgaccgc aatatcaccg gtatcgtgat
     4321 ggatctgaaa aacttcgcag gcggcgacca gccgtctatg cagtacatcg gcaaagccct
     4381 gaaagagttt cgtgacagcg ggaaaccggt ttatgccgtt ggcgagaact acagccaggg
     4441 gcaatattat ctcgccagtt tcgccaataa aatttggctg tcaccgcaag gcgtggttga
     4501 tctgcacggt tttgctacca acggtctgta ctacaaatcg ttgctggata agctgaaagt
     4561 ttccacccat gtgttccgcg tgggtacgta taaatctgcc gttgaaccgt ttattcgtga
     4621 tgatatgtca ccggcagccc gcgaagctga cagccgctgg attggtgagc tgtggcaaaa
     4681 ctatctgaat actgttgccg ctaaccggca gatccctgct cagcaggtat tccctggcgc
     4741 gcaagggttg cttgagggtt taaccaaaac cggtggcgat accgcgaaat atgcactgga
     4801 aaacaagctg gtcgatgcac tggcatcgag tgccgaaatc gaaaaagctc tgaccaaaga
     4861 gttcggctgg agtaagactg ataaaaatta tcgcgccatc agttattacg attacgcatt
     4921 gaaaacgccg gcagataccg gtgacagcat cggtgtcgtc ttcgctaatg gcgcaattat
     4981 ggatggcgag gaaactcagg ggaatgttgg cggtgatacc actgcggcac aaatccgcga
     5041 cgctcgcctt gacccgaaag tgaaagcgat tgtcctgcgt gttaatagcc caggcggcag
     5101 cgttaccgcg tctgaagtga ttcgcgctga actggcagca gcccgggcag cgggtaagcc
     5161 tgtggttgta tcgatgggcg gcatggcggc atctggtggt tactggattt ccacgccagc
     5221 taattacatt gtggctaacc ccagcaccct gaccggttct atcggtatct tcggcgtgat
     5281 caccaccgta gaaaatagtc tggattcgat tggtgttcat actgatggtg tctcaacttc
     5341 accgctggcg gatgtttcta tcaccagggc actgccgccg gaagcgcagc agatgatgca
     5401 attaagcatt gagaatggct ataaacgctt tatcacgctg gttgctgatg cgcgtcattc
     5461 gacgccggag caaattgata aaatcgccca gggccacgtc tggaccggtc aggatgcaaa
     5521 agctaacggg ctggtcgata gtctcgggga tttcgatgat gcggttgcca aagcagcaga
     5581 gctggcaaaa gtgaaacagt ggcatctgga atactacgtt gatgaaccga ccttcttcga
     5641 caaagtgatg gacaacatgt ctggttctgt ccgggcaatg ttgccagatg cgttccaggc
     5701 catgttacct gcaccgcttg cctcggtagc ctctaccgtt aaaagtgaaa gcgacaagct
     5761 ggccgcgttt aacgacccac aaaaccgtta tgcgttttgc ctgacctgcg ccaacgtgcg
     5821 ttaagtcttg tactgagtgg ccgacagatc gtcggccaca ttatttttta cctcgacgaa
     5881 tcctcttccc gctgtctcga cccatatttc cttatccgca tagtatcagg tgcgctcctc
     5941 ctgcctcacg tatatacttt tgctctttcg atatcattca tatcaatatc atgcaaaaga
     6001 aatcaattta cgtcgcctac acgggcggga ccatcgggat gcagcgttcc gagcagggtt
     6061 atattccggt gtcaggtcat ctacaacgcc aactggcgct gatgccggaa ttccatcgcc
     6121 cggagatgcc agatttcacc attcatgaat atacgccgtt gatggattct tctgatatga
     6181 cgccggaaga ctggcagcat attgctgaag atattaaagc gcactatgac gactatgatg
     6241 gttttgtcat tctacacggt accgacacga tggcgtatac cgcctctgcg ctgtcgttca
     6301 tgctcgagaa tctcggtaaa ccggtcattg tgacagggtc acaaatcccg ctggctgagt
     6361 tacgctctga cggacaaatt aatctgctga atgcgttgta cgttgcggcg aattatccga
     6421 tcaacgaagt aacgctcttt ttcaataacc gattgtatcg cggcaaccgc actaccaaag
     6481 cccatgccga tggttttgat gcgtttgcct ctccaaacct tcctccgtta ctggaagctg
     6541 gtatccatat tcgtcgtttg aatacgccac ccgccccgca cggtgaaggg gaattaatcg
     6601 ttcatccaat cacgccacaa ccaattggcg tagtgacgat ttatccaggg atttctgctg
     6661 acgtcgtgcg caattttctg cgccaaccgg tgaaagcatt gattctgcgc tcctatggcg
     6721 tgggtaatgc gccacaaaac aaagccttcc tgcaggaatt acaagaagcc agcgatcgcg
     6781 gtattgtggt ggtcaacctg acacaatgta tgtccggtaa agtgaatatg ggtggttatg
     6841 ccaccggtaa cgccctcgcc catgccggcg ttattggcgg tgcagatatg actgtagaag
     6901 ccacgctaac caaactgcat tacctgctga gccaggaact ggatactgaa accattcgca
     6961 aggccatgag ccaaaacctg cgcggcgaac tgacgccgga tgattaagga gactgcaatg
     7021 ccccatcgcg ccctgttact ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc
     7081 gccgtgccgg aaggtgacag tacggtagat gtcgctaacc gcctgattga ctggtgccag
     7141 tcgcgcggtg aagcggttat cgccagtcag gactggcacc cggcgaatca cggcagtttt
     7201 gccagtcagc acggtgtaga gccttatacg ccaggccaac tcgacggttt gccacaaacc
     7261 ttctggccag atcactgtgt gcagaacagt gaaggcgcac aattacatcc gttactgaac
     7321 caaaaagcga tcgcagcggt gttccataaa ggcgaaaatc ctttagttga cagttacagt
     7381 gccttttttg ataacggccg tcggcagaaa acctctctcg atgactggtt acgcgatcat
     7441 gaaatcgatg aattgatcgt tatgggcctg gctactgact attgcgtgaa gtttaccgtg
     7501 ctggacgcgt tacagttagg ttataaggta aacgtgatta ccgatggttg tcgtggcgtg
     7561 aatatccagc cccaggacag tgcgcacgcg tttatggaga tgtcagcagc tggggcaacg
     7621 ctatatacgc tggcagactg ggaagagacg caggggtaat attagttgaa ttcatctctg
     7681 gcctacattt cctcaatgac attgtaggcc agataatact tgtcagtgtt gcatttaacc
     7741 tacttgcagc taaaacacga cgtcctaaca agtatatttg ccctccctta ataacaggct
     7801 taaatactag ataactcatt cacttaaaca tagcaatagg ctttgctgag ataccaaaat
     7861 cttcttttaa ctgctgtttg ctcttcatga ccatctgacc atttgtgtca attgtcatat
     7921 gctgtgcttc ggtattatgg cgggcttgcc acagcatcac cagttgcagg caattctctt
     7981 tttgttcctc ggtcaacgca acgccatcag gccatttccc cagttcaacg gcggtcgaca
     8041 aacgctggta tacctcaggc gtcatgctgt tgataatgtc atcaagattc ataatttttc
     8101 cgctcctgtg gaataagttg ctgaatcgtt ttttcaaccg ttgatttctt cgccgttttc
     8161 gccatcagtg aagcgtaaag aagcagagtt aacacaataa cgttcgcctg ttggttgcgg
     8221 cccgtcggga aagacatgcc ccagatgggc atcacagtta ccgcaacgaa tttctatgcg
     8281 ctgcattcca tgtgacaagt ctttgatata acgaatggat tcttcactca ccggttcgta
     8341 gaaactgggc cagccacagc cggaatcata cttggtttcg gaatgaaaca gcggggcatc
     8401 gcagatcaaa cagtgatata cgccgtcacg cttgttatgc agtaaacgac ccgtaaatgg
     8461 cggttctgtc ccatgattct gcgtcacgta aaactgcatc tcggacaaat tttttttcag
     8521 ttcttctgcc gaaggtttat tagccatttg ctcacatctc actttaatcg tgctcacatt
     8581 acgtgactga ttctaacaaa acattaacac caactggcaa aattttgtcc taaacttgat
     8641 ctcgacgaaa tggctgcacc taaatcgtga tgaaaatcac atttttatcg taattgccct
     8701 ttaaaattcg gggcgccgac cccatgtggt ctcaagccca aaggaagagt gaggcgagtc
     8761 agtcgcgtaa tgcttaggca caggattgat ttgtcgcaat gattgacacg attccgcttg
     8821 acgctgcgta aggtttttgt aattttacag gcaacctttt attcactaac aaatagctgg
     8881 tggaatatat gactatcaaa gtaggtatca acggttttgg ccgtatcggt cgcattgttt
     8941 tccgtgctgc tcagaaacgt tctgacatcg agatcgttgc aatcaacgac ctgttagacg
     9001 ctgattacat ggcatacatg ctgaaatatg actccactca cggccgtttc gacggtaccg
     9061 ttgaagtgaa agacggtcat ctgatcgtta acggtaaaaa aatccgtgtt accgctgaac
     9121 gtgatccggc taacctgaaa tgggacgaag ttggtgttga cgttgtcgct gaagcaactg
     9181 gtctgttcct gactgacgaa actgctcgta aacacatcac cgctggtgcg aagaaagtgg
     9241 ttatgactgg tccgtctaaa gacaacactc cgatgttcgt taaaggcgct aacttcgaca
     9301 aatatgctgg ccaggacatc gtttccaacg cttcctgcac caccaactgc ctggctccgc
     9361 tggctaaagt tatcaacgat aacttcggca tcatcgaagg tctgatgacc accgttcacg
     9421 ctactaccgc tactcagaaa accgttgatg gcccgtctca caaagactgg cgcggcggcc
     9481 gcggcgcttc ccagaacatc atcccgtcct ctaccggtgc tgctaaagct gtaggtaaag
     9541 tactgccaga actgaatggc aaactgactg gtatggcgtt ccgcgttccg accccgaacg
     9601 tatccgtagt tgacctgacc gttcgtctgg aaaaagctgc aacttacgag cagatcaaag
     9661 ctgccgttaa agctgctgct gaaggcgaaa tgaaaggcgt tctgggctac accgaagatg
     9721 acgtagtatc taccgatttc aacggcgaag tttgcacttc cgtgttcgat gctaaagctg
     9781 gtatcgctct gaacgacaac ttcgtgaaac tggtatcttg gtacgacaac gaaaccggtt
     9841 actccaacaa agttctggac ctgatcgctc acatctccaa ataagttgag atgacactgt
     9901 gatctaaaaa gagcgacttc ggtcgctctt ttttttacct gataaaatga agttaaagga
     9961 ctgcgtcatg attaagaaaa tttttgccct tccggtcatc gaacaaatct cccctgtcct
    10021 ctcccgtcgt aaactggatg aactggacct cattgtggtc gatcatcccc aggtaaaagc
    10081 ctcttttgca ttacagggcg cacaccttct ctcgtggaaa cctgcgggtg aagaagaagt
    10141 tctgtggttg agcaacaaca caccgttcaa aaatggcgtc gctattcgcg gtggcgtacc
    10201 ggtttgctgg ccgtggtttg gtccggcggc acaacaaggt ctgcctgcgc acggttttgc
    10261 ccgcaacctg ccgtggacgc tgaaatcaca tcgtgaagat gctgatggcg tagcgctgac
    10321 ttttgaattg acgcaaagcg aagagacgaa aaaattctgg ccgcacgact ttacgctgtt
    10381 agcgcatttc cgcgtgggta aaacctgtga aatcgatctt gaatcacatg gcgaatttga
    10441 aaccacctct gccctgcata cctactttaa cgtgggtgat atcgctaagg taagcgtcag
    10501 tgggctgggc gatcgcttca ttgataaagt aaatgacgcg aaagaagatg tactgaccga
    10561 cggtattcag accttccctg accgtaccga tcgcgtgtat ctgaatccgc aagattgcag
    10621 cgtgattaat gatgaagcgc tgaatcgtat tatcgccgta ggccaccagc atcatctgaa
    10681 cgttgtcggc tggaacccgg gaccggcgct ttcagttagc atgggcgaca tgccggatga
    10741 tggctacaaa acatttgttt gtgtagaaac ggcttacgct tcagaaacgc aaaaagtgac
    10801 caaagagaaa cctgcacatc tggcgcaatc cattcgcgtt gcgaaacgtt aatttgcgtt
    10861 aatgttgtgt gccgggtgcg atgcatccgg cacgtatcaa catcacacca tatccagcgc
    10921 agtttttcct tttggtgccg gatacgcttt atccagcatg gttaattccg ctgacgaaag
    10981 ttcgacctca agcacagccg cattctgttg gacatgggca atcgtggccg cttttggaat
    11041 cgccatcaca ccctgatggc tgattaccca cgccaacaat acttgtgccg cgctgatatt
    11101 gtgagcatgt gcaatttcgt tgactaccgc gttttttaac agtccattgc gcaaccgccc
    11161 ggcctgggct aatggactgt aagccatcac cggcatctgc tgttgctggc accaggggag
    11221 tagatcgtac tcaatccctc gtgaaccgag atggtaaagc acctgattag tggcacactg
    11281 atttcccccc ggcagctgcc agagttcctg catatcagca tgatcaaggt tagaaacgcc
    11341 ccagcggcgg atttttccct gggcgatcaa tttttccatc gctgccacag tctcttcaaa
    11401 agcgaaactg ccagaccagt gtaataagta gagatcgaga taatcagtat tgagacggcg
    11461 taaactggct tcgcatgcat ttatcgcttt ttgcccgcca gcattccacg gatagacttt
    11521 agagacgaga aagacgttat cgcgcagacc ggttaatgct tccccaacca ccttttcggc
    11581 accgccatcg gcatacattt cggcggtatc aatgagggtt aaaccgagct caatgcccgc
    11641 gcgtagtgca gcaacttctg ttttgcgctg actggcatct tcgcccatat accatgttcc
    11701 ctgccctatg gctggcagtg agacatcgcc actaaattga atcatttttt gttgcattgt
    11761 ttcctcccag gtattgcacc accgtaatgc aaaacagggc gtgacgccct gtttttatgc
    11821 acaaaatgcc ctggaaagat gcattatcag aatttgtagg tgatcccggt agaaatcagg
    11881 ccagtccagg atttatccac catcgggctg tcagtaactt catcagacag acgggtatag
    11941 cgcgcggtac cgtaaacact ccagtcgccg aggaagttgt agctggcgct cagctccagg
    12001 taagggctcc agctgtcgtt cgggttatag ccacgcagac cgctgcgagc ggactctttg
    12061 cgcgatacgc cataatagta ttcgttctgg ttttcgctgt tccactgcac accgataccc
    12121 ggggtcacgg tcaggccgcc gttggtgtaa cgatacaacc aggccatatc ccagacgatg
    12181 ccgttgctgt tatccagggt atcgccagcc agggtggtac gcaggtaacc gtactgggta
    12241 aagtgagcat aagacagacc agccatcatg gtgctcttac ggtcatccag gtgacgcatt
    12301 tggtgatcgc cactgtcttt cgctttgaag taaagcggcg accaataagc ggtaattgaa
    12361 agtttgtccg ttgcgtcatt ccacaggtag taaccaccac ctaagccacg gaaccagaag
    12421 ttatcgcctt catagttgat taccggtact gggtaaacat cggtatcgta atctttatat
    12481 gggtgctcaa cgacacctac gcctgcgccc agggaaaatt taccttcagc gtgcgctacg
    12541 cctgcagacg ttgcgataag cactccaagt gccagaagtt tgagtttggt cacaattaat
    12601 cattccttaa acaaatgttt agcgggcgac aaagtttacc cgtcaataca tcgaaaccca
    12661 acctttttac gttttcattt tttaaagtaa ctgtttaatt ttcctgacgc ggatgacacc
    12721 gcgcttacag ccaaatgaat ttagcgttac tggctaccct ggtctttaca ttaattatgc
    12781 aaaatttatg gatgagttgt tgatatgcca ttgaaattaa gaaagccgtg caggcaagtt
    12841 ttccatttgc catctacgct taattttgaa ggtgtatcac cgggcacgtt gttctcatcg
    12901 tcgataaaat ggcatgagag ttgctgtgtt ttagcaagag acgtcgttca gtttacctct
    12961 tccgggagcc tctactattc atatgaacgg ctcttaacct gtgctaaaaa acgaaaggac
    13021 ggcataccat gaatatattc gatcactatc gccagcgata tgaagctgcc aaggacgaag
    13081 agttcacact gcaggagttt cttaccactt gtcggcaaga tcgcagtgct tatgccaacg
    13141 cggctgagcg gctattgatg gctatcggtg agcctgtcat ggtcgataca gcccaggaac
    13201 ccagactttc tcgactcttt tctaaccggg tcattgcacg ttatccggca tttgaagagt
    13261 tttacggcat ggaagacgcg attgaacaga ttgtctctta tctgaaacac gcggctcagg
    13321 ggctggaaga gaagaaacaa atcctgtatc tgctggggcc tgtgggtggg ggtaaatcat
    13381 cgcttgctga gcgactgaaa tcattaatgc agctcgtacc gatttatgta ttgagcgcca
    13441 acggtgagcg tagcccggtc aacgatcatc cgttctgtct tttcaatccg caggaagatg
    13501 ctcagattct ggaaaaagag tatggcattc ctcgccgtta tctcggcacc atcatgtcgc
    13561 catgggcggc aaaacgcctg catgaatttg gtggcgatat cactaagttc cgggtagtga
    13621 aggtctggcc gtcaattctg caacaaattg ctatcgccaa aacggaaccc ggtgatgaga
    13681 acaaccagga catctccgcg ctggttggga aagtcgatat tcgtaaactc gaacactacg
    13741 cgcagaatga cccggacgcc tacggctatt ccggtgcgct gtgccgcgcc aaccaaggga
    13801 tcatggaatt tgtcgagatg tttaaagcac cgataaaagt gctgcatccg ctgttaaccg
    13861 ccactcagga aggtaactac aacgggacgg aaggtatctc cgccctgccg ttcaacggga
    13921 ttattctcgc ccactcgaac gaatccgaat gggtcacttt ccgtaataac aaaaacaacg
    13981 aagccttcct cgaccgtgtt tacatcgtga aggtgccgta ttgcttacgc atttccgaag
    14041 agatcaaaat ctacgagaaa ttgcttaatc acagtgaatt gactcacgcc ccctgcgccc
    14101 ctggcacgct ggaaacgctg tcacgttttt ccattctttc gcgccttaaa gagccagaaa
    14161 actccagcat ttattcaaag atgcgggttt atgatggcga aagtctgaaa gacaccgatc
    14221 ccaaagccaa gtcgtatcag gaatatcgtg actacgccgg tgtcgatgaa gggatgaacg
    14281 gtctgtcgac gcgttttgcg tttaagatcc tctcccgcgt gttcaacttc gatcatgtag
    14341 aagtggcggc aaacccggtc catctgttct acgtcctgga acagcagatc gagcgcgagc
    14401 agttcccaca agagcaggca gaacgctatc tggagttcct gaaaggttat ctgatcccga
    14461 aatatgccga gtttatcggc aaagagatcc agacggccta ccttgaatcc tattccgaat
    14521 atgggcaaaa cattttcgac cgttatgtta cctacgcgga tttctggatt caggatcagg
    14581 agtatcgcga tccggatacc gggcagctgt ttgaccgcga gtctcttaac gccgagctgg
    14641 agaaaatcga gaaaccggcg gggatcagta atccaaaaga tttccgcaac gagattgtta
    14701 acttcgtact gcgcgccaga gcgaataaca gcggacgcaa tccgaactgg accagctatg
    14761 aaaaactgcg cacggttatc gagaagaaaa tgttctccaa taccgaggag ctgttgccgg
    14821 ttatctcgtt taacgccaaa acgtcaaccg acgagcagaa gaaacacgac gactttgtcg
    14881 accgtatgat ggaaaaaggc tacacccgta aacaggtgcg tttactgtgc gaatggtatt
    14941 tgcgcgtacg taaatcgtct taacaaccct ggcccggtac acatgttacc gggcctacaa
    15001 cgacagcgaa ccgtgggcct gagaagcggc aacacaggcg tagcatacag ttggcaaatg
    15061 tagtacgggg ggcatatgac ctggtttatt gaccggcgtc tgaacggcaa aaacaaaagc
    15121 atggtgaatc gccagcgttt tttacgccgt tataaagcgc aaattaaaca gtcgatctcc
    15181 gaggccatta ataagcgttc ggtgactgac gtcgacagcg gcgaatccgt atccattccc
    15241 acggaagata ttagcgaacc gatgtttcat caggggcgtg gcggtctgcg ccaccgcgtg
    15301 catccgggca atgaccattt cgtccagaac gaccgaattg aacgtcccca gggtggcggc
    15361 ggaggttccg gtagtggtca gggccaggcc agccaggatg gtgaaggtca ggatgaattt
    15421 gtctttcaga tttcgaaaga tgagtatctt gatttgctct ttgaagattt ggccttaccg
    15481 aatctgaaac aaaaccaaca acgccagctg accgaatata aaacgcatcg ggcgggttat
    15541 accgctaacg gcgttccggc aaatatcagc gttgtgcgct ccttgcagaa ctcactggcg
    15601 cgacgcacag ccatgacggc aggcaagcgg cgggaacttc atgcactgga agagaatttg
    15661 gccatcatca gcaacagtga acctgcgcag ctgctggaag aggaacgtct gcgcaaagaa
    15721 attgcagaat tacgtgccaa aattgaacgc gtccctttta ttgacacctt cgatttacgt
    15781 tacaagaact acgagaagcg gcccgatccc tccagccagg cagtgatgtt ttgcctgatg
    15841 gacgtttccg gttcaatgga tcaatccact aaagatatgg ctaagcgttt ttatattctg
    15901 ctgtatctgt tcctcagcag aacgtataag aacgtggaag tcgtatacat ccgccatcat
    15961 acccaggcga aagaagtcga tgaacatgag tttttctact cgcaggaaac aggcggcacc
    16021 attgtttcca gcgccctgaa actgatggat gaggtagtga aagagcgtta taacccggca
    16081 cagtggaata tttacgctgc acaagcatcg gacggcgata actgggccga tgactctccg
    16141 ctttgccatg aaatcctggc gaaaaaatta ttacctgttg ttcgttatta cagctatatc
    16201 gaaattaccc gtcgtgcaca tcagacattg tggcgagaat atgagcatct gcaatctact
    16261 ttcgacaact ttgcgatgca gcacatccgc gaccaggatg atatttatcc ggtgttccgt
    16321 gaactgtttc ataaacaaaa tgcaacagct aaagactaaa actatcagcc aggtcattat
    16381 cgcctggctg atttttagct tactgtaaat tatctcattt attacataca ttagcttaca
    16441 atcgctttaa atatgacagc ggaaccttta cataatttag ttccagaaaa caatcattcg
    16501 gaaaaatgat tcagtcaaca cgtatttcca tggggttatt ctttaaatat tgtttatcgt
    16561 taacgaaaat tgatcctggt caaaactata tatctctgcc atcaataaaa tccagcactc
    16621 acattgctct cctttttatg gtttctatgg gtacacaaaa attaaaagct caaagctttt
    16681 ttattttcag tttattgctg acgttaattt tattttgcat caccacatta tataacgaaa
    16741 acacaa
//
LOCUS       DAZFJF010000101        16709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFJF010000101 DAZFJF010000000
VERSION     DAZFJF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.101"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..559)
                     /gene="ybiA"
                     /locus_tag="ACHKAU_003630"
     CDS             complement(77..559)
                     /gene="ybiA"
                     /locus_tag="ACHKAU_003630"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO5532617.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            679..2829
                     /gene="dinG"
                     /locus_tag="ACHKAU_003631"
     CDS             679..2829
                     /gene="dinG"
                     /locus_tag="ACHKAU_003631"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO5532618.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2857..3819
                     /gene="ybiB"
                     /locus_tag="ACHKAU_003632"
     CDS             2857..3819
                     /gene="ybiB"
                     /locus_tag="ACHKAU_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO5532619.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3960..5045
                     /gene="hcxB"
                     /locus_tag="ACHKAU_003633"
     CDS             3960..5045
                     /gene="hcxB"
                     /locus_tag="ACHKAU_003633"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO5532620.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5112..5171
                     /gene="ybiE"
                     /locus_tag="ACHKAU_003634"
     CDS             5112..5171
                     /gene="ybiE"
                     /locus_tag="ACHKAU_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO5532621.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5276..6640)
                     /gene="rhlE"
                     /locus_tag="ACHKAU_003635"
     CDS             complement(5276..6640)
                     /gene="rhlE"
                     /locus_tag="ACHKAU_003635"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO5532622.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            6869..7540
                     /gene="cecR"
                     /locus_tag="ACHKAU_003636"
     CDS             6869..7540
                     /gene="cecR"
                     /locus_tag="ACHKAU_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO5532623.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            7543..8538
                     /gene="hlyD"
                     /locus_tag="ACHKAU_003637"
     CDS             7543..8538
                     /gene="hlyD"
                     /locus_tag="ACHKAU_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO5532624.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            8531..10267
                     /gene="ybhF"
                     /locus_tag="ACHKAU_003638"
     CDS             8531..10267
                     /gene="ybhF"
                     /locus_tag="ACHKAU_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5532625.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            10260..11393
                     /gene="ybhS"
                     /locus_tag="ACHKAU_003639"
     CDS             10260..11393
                     /gene="ybhS"
                     /locus_tag="ACHKAU_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5532626.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            11404..12510
                     /gene="ybhR"
                     /locus_tag="ACHKAU_003640"
     CDS             11404..12510
                     /gene="ybhR"
                     /locus_tag="ACHKAU_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5532627.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12472..12882)
                     /gene="ybhQ"
                     /locus_tag="ACHKAU_003641"
     CDS             complement(12472..12882)
                     /gene="ybhQ"
                     /locus_tag="ACHKAU_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO5532628.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            13015..13776
                     /gene="ybhP"
                     /locus_tag="ACHKAU_003642"
     CDS             13015..13776
                     /gene="ybhP"
                     /locus_tag="ACHKAU_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5532629.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            13773..15014
                     /gene="clsB"
                     /locus_tag="ACHKAU_003643"
     CDS             13773..15014
                     /gene="clsB"
                     /locus_tag="ACHKAU_003643"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO5532630.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            15014..15970
                     /gene="ybhN"
                     /locus_tag="ACHKAU_003644"
     CDS             15014..15970
                     /gene="ybhN"
                     /locus_tag="ACHKAU_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO5532631.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16006..>16709)
                     /gene="ybhM"
                     /locus_tag="ACHKAU_003645"
     CDS             complement(16006..>16709)
                     /gene="ybhM"
                     /locus_tag="ACHKAU_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5532632.1"
                     /translation="SQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYLEITA
                     TKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAPVPAI
                     IVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERPVWVI
                     SCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFGILAA
                     IAITLVWQRHTRFFH"
BASE COUNT         3886 a         4364 c         4592 g         3867 t
ORIGIN      
        1 acaacaagca cttgtcagca atttgagaac acgggaaaaa atttatctgt tttaccgccc
       61 ataagccacc gagacgttac ttctctatag ccaattgttc gcgcaactcc attaaaaggt
      121 agcccagtcg attcttgccc ttaccattac caccgtctcc ccagtaagca tcgttttccg
      181 tatgctcaac cagttttgcg ggcgcggttg ccagcaagag cgcacgcagt tctggatgct
      241 gttcgaattt agcgcgaagt gcttttcgca tcacctgttc tttgaccgac tcccagtttt
      301 tacgcagagg cttagaacga tcgcgcccca tgcgtgcggc gaccataggg gaagaaaccc
      361 gacggatctc ttcgcggtat ttttcatcaa ggaatttttg tgcctgaaaa tagtgttctg
      421 aggtgggcca ggttttcccg tcaaccttga tgggccaggc ggcaaagtta gaaaaatcac
      481 cgtagtcatc gctggtgctg tagaaattta tgatggtgtc ttgcatgacg tgttggatcc
      541 tttgtgctcg aacgggcatt aaaccgcatt atgttggtgg ttattgcgag ccgctttcca
      601 gaaacagaaa aaccattacc cctgaaaacc gaaaaatgcc acaatattgg ctgtttatac
      661 agtatttcag gttttctcat ggcattaacc gccgcgctta aagcgcaaat tgccgcctgg
      721 tataaggcgc ttcaggaaca gatccccgac tttattcccc gtgcgccgca gcggcagatg
      781 attgcggacg tcgccaaaac gctggccgga gaagaagggc gacatctggc gattgaagcc
      841 cccaccggcg ttgggaaaac gctctcctat ttgatccccg gcatcgccat tgcccgcgaa
      901 gagcaaaaaa cgctggtggt gagtaccgcc aacgtggcat tgcaggatca gatttacagc
      961 aaagatttac cgctgctgaa aaagatcatt cccgatctta aattcactgc cgcttttggg
     1021 cgtgggcgct acgtttgccc gcgtaatctg acggcgctcg ccagcactga gcccacgcaa
     1081 caggatctgc tggcgtttct tgacgacgaa ctgacgccaa ataatcagga agagcaaaaa
     1141 cgttgtgcga agctgaaggg cgatctcgac acttataaat gggatggtct gcgcgatcat
     1201 actgatatcg ctatagatga cgatctctgg cgtcgtttaa gtaccgacaa agccagctgc
     1261 ctcaaccgca actgttacta ctatcgtgaa tgcccgtttt ttgttgctcg tcgggagatt
     1321 caggaagcgg aagtggtggt ggcaaaccat gcgctggtga tggcggcgat ggaaagcgaa
     1381 gccgtactac ctgacccgaa aaatctgctg ctggtgctgg acgaaggtca tcacctgcca
     1441 gatgtggccc gggatgcgct ggagatgagt gccgaaatca ccgcgccctg gtatcgacta
     1501 cagctggact tgttcacgaa actggtcgct acctgcatgg agcagtttcg cccgaagacc
     1561 atcccaccgc tggcgatccc tgaacgtttg aatgcgcatt gtgaagagtt gtatgagctt
     1621 atcgcctcgt taaacaacat tctcaatctc tacatgcctg ccgggcagga ggcagagcac
     1681 cgttttgcga tgggcgaact gcctgatgaa gtgctggaga tctgccagcg gctggcaaaa
     1741 ctcaccgaga tgctgcgtgg tctggcggag ttatttctta acgatttaag tgagaaaacc
     1801 ggcagccacg atattgtgcg tctgcatcgg ttgattttgc agatgaaccg cgcgttgggg
     1861 atgttcgagg cgcaaagcaa actctggcgg ctggcgtctc tggcgcaatc ttccggtgcg
     1921 ccggtgacca aatgggcgac gcgggaagag cgcgaagggc agctgcatct ctggtttcac
     1981 tgcgtgggaa tacgtgtcag cgatcagctg gaaaggctgc tgtggcgcag tattccgcac
     2041 attattgtca cctccgcaac cttgcgttcg ctgaacagtt tttcgcgttt gcaggagatg
     2101 agtggtctga aagagaaagc gggcgaccgt tttgtggcgc tggattcccc ctttaaccac
     2161 tgcgaacagg gcaaaattgt tattccccgg atgcgctttg agccttccat cgacaacgaa
     2221 gagcagcata ttgccgaaat ggcggccttt ttccgtaagc aggtggagag caaaaaacat
     2281 ctcggtatgt tggtactgtt tgccagcgga cgggcgatgc agcgctttct cgactatgtg
     2341 acggatttac gcctgatgct gctggtgcag ggcgatcagc cgcgttatcg cttagttgaa
     2401 ctgcaccgca aacgcgttgc caacggtgag cgtagtgtgc tggtgggctt acagtcattt
     2461 gccgaagggc ttgatttgaa aggtgatctg ctcagccagg tgcatatcca caaaatcgct
     2521 tttccgccca tcgacagccc ggtggtgatc accgaagggg aatggctgaa aagcctcaac
     2581 cgctatccgt ttgaggtgca aagcctgccg agcgcctcgt ttaacctgat tcagcaggtt
     2641 gggcgactga ttcgaagcca cggttgctgg ggcgaagtgg ttatctacga caaacgcttg
     2701 ctgaccaaaa attatggcaa gcgactactg gatgcattac cggtatttcc gatagagcaa
     2761 ccggaagtcc ctgaaggtat agttaaaaag aaagaaaaaa cgaaatcccc acgccgtcgg
     2821 cggcgttaat gatgtgagtc aggtaaggag tcgtaaatgg actatcgcaa aatcattaaa
     2881 gagatcgggc gcgggaaaaa ccacgcgcgc gatttagacc gggatactgc gcgcggtctg
     2941 tatgcccata tgctcaatgg tgaagtccct gaccttgagt tgggcggcgt attgattgcg
     3001 ctgcgtatca aaggggaagg ggaagcagag atgctcggct tttacgaagc catgcaaaat
     3061 cacaccatca agctgacgcc gcctgcaggc aagccgatgc cgattgtcat ccccagttac
     3121 aacggcgcgc gtaaacaggc caacctgacg ccgttgttgg cgattctcct gcacaaactc
     3181 ggttttcctg tggtggttca cggggttagc gaagatccaa cccgcgtgct gactgaaacc
     3241 atttttgaat tgatgggtat tacgccaacg ttgcacggcg gacaggcgca ggcgaagctc
     3301 gacgaacatc aaccggtgtt tatgccagtc ggcgcgtttt gcccgccgct ggaaaaacaa
     3361 ctggcgatgc gctggcggat gggcgtgcgt aacagtgcac ataccctggc gaaactggca
     3421 acgccatttg ccgaaggtga ggcgctgcgt ctttccagcg tttcgcatcc ggaatacatt
     3481 ggacgcgtcg cgaagttctt tagcgatatc ggcgggcggg cgctactaat gcatggtact
     3541 gaaggtgaag tgtatgctaa tccgcagcgc tgcccgcaga tcaatctcat tgaccgtgaa
     3601 gggatgcggg tgctgtatga aaaacaggac actgctggta gcgagttact gccacaagca
     3661 aaagatccgg aaaccacggc gcagtggatt gagcgttgcc ttgctggcag cgaaccgatt
     3721 cccgaatcgc tgaaaatcca gatggcttgc tgcctggtgg ctacgggtga agcggcaact
     3781 atcagcgacg gcctggcgcg cgttaatcag gcattttaat tcttttctca gccggatgag
     3841 ccatgtcatc cggcttttac cccaccgcaa tatgaaattc ctgcatcttt attgaccttc
     3901 ccacgcccgg cgtgcagcat aaaaatacaa caaacacata acataaacag gagttaacca
     3961 tggaaagtgg tcatcgcttt gatgctcaga cgctgcacag ttttattcag gctgtatttc
     4021 gtcagatggg tagcgaggaa caagaagcga aattagttgc cgatcattta atcgcggcaa
     4081 acctggcagg gcatgattca catggtattg gcatgatccc aagctatgta cgctcctgga
     4141 gtcaggggca cctgcaaatt aaccatcatg ccaaaaccgt taaagagtcg ggggcggcgg
     4201 tcacgctcga tggcgatcgc gcatttggtc aggtcgcggc acatgaagcg atggcgctgg
     4261 ggattgagaa agcgcataag cacggtattg ccgccgtggc gctacataac tcgcatcata
     4321 tcggccgtat cggttactgg gcggagcagt gtgcagcggc ggggtttgtc tctatccact
     4381 ttgttagcgt ggtcggtatt ccaatggtcg cgccgttcca cggtcgcgac agccgctttg
     4441 gcaccaatcc gttctgtgtg gttttccctc gtaaagataa tttcccgctg ttgcttgatt
     4501 acgccaccag cgccattgca tttggtaaaa ctcgcgtcgc ctggcataaa ggcgtccccg
     4561 tgccgccagg ttgcctgatt gacgttaacg gcgtgccgac gaccaatccg gcggtaatgc
     4621 aggagtcgcc gttgggggcg ctgttgacct ttgccgaaca taaaggctac gcccttgcag
     4681 cgatgtgtga aattcttggc ggggcgcttt ctggcggtaa aacgacgcat caggaaacgt
     4741 tacaaaccag tcccgatgcc attcttaact gcatgaccac tatcatcatc aacccggaac
     4801 tcttcggcgc gccggattgt aacgcgcaga ccgaagcctt tgccgagtgg gtgaaagcct
     4861 cgccgcatga tgatgataag ccgattttgc taccgggcga gtgggaagtg aacacgcgtc
     4921 gcgaacggca gaagcagggg attccactgg atgcgggaag ctggcaggcc atttgtgatg
     4981 cagcgcggca gattggtatg ccggaagaga cgttgcaggc tttctgtcag cagttagcca
     5041 gctaaaaaaa agcccgtcca gtggcggacg ggcaaacaag ggtaacatag gatcaatgag
     5101 ggttagagca tatgcgtctg tcggcaaaca gacagggaaa tacttgtgct ggacgtagcg
     5161 taaacgcctg atccgtccaa cggttcggat tttgtagata ctgataagcg tagcgcatca
     5221 ggcagttttg cgtttgttca tcagcctgat gccgggcata gcccggcata aaagattact
     5281 gcgcagcggt aggtttacgc ggacggcggc gacgttgttg ttcgcctgcc ggtttggcat
     5341 cgccgaggcg gcgagacggt ttttctgcag gtttcgcgct tgcagatttt gcgccacctt
     5401 ccccacggcg tggttgctgt tgaccgcgac caccaccttg cccacgaccg ccgtcgccac
     5461 gttgctggcg accgttctgg atcggttcgg ctttgattga cgggtccggc tcatagcccg
     5521 gaatcgcaat gcgcgggatc tcttttttca gcagtttttc gatatcacgc agcagtttgt
     5581 gttcatcaac acacaccagc gacaacgctt caccggtagc agccgcacga ccggtacgcc
     5641 cgatacggtg gacataatct tccggtacgt ttggcagttc atagttgacg acgtgcggca
     5701 actcttcgat atccagaccg cgcgcagcga tatcggtcgc caccagtacg cgaatgtcac
     5761 ccgatttaaa atcagccagc gcacgggtac gcgcaccttg cgatttattc ccgtggatcg
     5821 ccgcactacg gatgccatct ttattgagct gctcagccag atggttagcg ccgtgtttgg
     5881 tacgggtaaa caccagcacc tgctgccagt tccctttacc aatcatgtgc gacagcaatt
     5941 cgcgtttgcg tttcttatca acaaagtgaa cgtgctgggt cacctgatca gacgcggtat
     6001 tgcggcgtgc cacttcgatt tccagcgggt tgtgcaacag tttttccgcc agggctttaa
     6061 tatcgtcaga gaaggtcgca gagaacagca ggttctggcg cttcgctggc agttttgtta
     6121 acacgcgacg gatatcgtgg ataaagccca tgtcgagcat gcggtcagct tcatcgagga
     6181 cgaggatttc aacctgatcc agcttcactg cattttgatg ttccaggtcc agcaaacgtc
     6241 ccggtgttgc caccagaaca tcaacgccgc cgcgcagctt catcatttgc gggttaatgc
     6301 tgacaccgcc aaacaccacc agcgaacgaa tgttcaaata tttgctgtaa tcacggacgt
     6361 tttcgccaat ctgcgcagcc agttcacggg tcggggtaag aatgagcgca cgtaccggac
     6421 gacgcccttt ggcgtgcggc tggcgagtga tcaggtgttg caacagcggc agcgtaaagc
     6481 ccgctgtttt accggtaccg gtctgggcgc tagccatcag gtcgcggcct tccagcaccg
     6541 cagggatcgc ctgctgctga ataggggtgg gttcacggta accctgctcg gcaacggcgc
     6601 gcaggatatc agggcttaaa cccaaagaat cgaaagacat aactactccg aaccgccccg
     6661 accgttaccg gtgtagtttt cagggagata ctgaaaagga aaatgacaaa aaccacaatg
     6721 tcatgaggga gcggagtgta gcagtttttg tgacgcagcg cataaattat ccctgtgact
     6781 ggcgtagcta aaattcttag ccatactgga caactcccat atggagtcat aatcttaatc
     6841 aatcatttga ttaagaatgg actgtgcgat gaataatcct gccatgacaa tcaagggtga
     6901 acaggcgaaa aaacagctga ttgctgccgc actggcgcag tttggtgaat atggaatgaa
     6961 cgccaccacc cgcgagatag ccgcccaggc cgggcagaat atcgctgcca tcacctacta
     7021 cttcggttcg aaagaagatt tgtacctcgc ctgcgcccag tggattgccg attttattgg
     7081 cgagcagttc cgtccgcacg ccgaagaggc cgaacgcttg ttcgcacaac cacagcctga
     7141 tcgcgcagcc atccgtgaac tgatccttcg cgcctgcagg aacatgatta agctgctcac
     7201 ccaggatgac accgtcaacc tcagcaagtt tatctcccgt gagcagctct ctcccacggc
     7261 agcctatcac ctggtgcacg aacaggtgat tagcccgcta cacagccacc tgacacgcct
     7321 gattgccgcc tggaccggct gcgacgccaa cgatacccgc atgatcctcc atactcatgc
     7381 gttgattggt gagattctgg cgttccgtct tggcaaagaa acaattctgt tacgtaccgg
     7441 ctggaccgcg ttcgatgaag aaaagaccga actgatcaac cagacggtga cctgtcatat
     7501 cgacctgatt ttgcaaggat tatcgcaaag gagtttgtag tgatgaaaaa acctgtcgtg
     7561 atcggactgg cggtagtggt acttgccgcc gtggttgccg gaggctactg gtggtatcaa
     7621 agccgccagg ataacggcct gacgctgtat ggcaacgtgg atattcgtac ggtgaatctt
     7681 agtttccgtg ttggggggcg cgttgaatcg ctggcggtgg acgaaggtga tgctatcaaa
     7741 gcgggccagg tgctgggcga actggatcac aagccgtatg agattgccct gatgcaggcg
     7801 aaagcgggtg tttcggtggc acaggcgcag tatgacctga tgcttgccgg gtatcgcgat
     7861 gaagaaatcg ctcaggccgc cgcagcggtg aaacaggcgc aagccgccta tgactatgcg
     7921 cagaacttct ataaccgcca gcaagggttg tggaaaagcc gcaccatttc ggcaaacgac
     7981 ctggaaaatg cccgctcctc gcgcgaccag gcgcaggcaa cgctgaaatc agcacaggat
     8041 aaattgcgtc agtaccgttc cggtaaccgt gaacaggaca tcgcccaggc gaaagccagc
     8101 ctcgaacagg cgcaggcgca actggcgcag gcggagttga atttgcagga ctcaacgttg
     8161 atagccccgt ctgatggcac gctgttaacg cgcgcggtgg agccaggcac ggtcctcaat
     8221 gaaggtggca cggtgtttac cgtttcacta acgcgtccgg tgtgggtgcg cgcttatgtt
     8281 gatgaacgga atcttgacca ggcccagccg gggcgcaaag tgctgcttta taccgatggt
     8341 cgcccggaca agccgtatca cgggcagatt ggtttcgttt cgccgactgc tgaatttacc
     8401 ccgaaaaccg tcgaaacacc ggatctgcgt accgacctcg tctatcgcct gcgtattgtg
     8461 gtgaccgacg ccgatgatgc gttacgccag ggaatgccgg tgacggtgca attcggtaac
     8521 gaggcaggac atgaatgatg ccgttatcac gctgaacggc ctggaaaaac gctttccggg
     8581 catggacaag cccgccgtcg cgccgctcga ttgtaccatt cacgccggtt atgtgacggg
     8641 gctggtgggg ccggatggtg caggtaaaac cacgctaatg cggatgctgg cgggattact
     8701 gaaacccgac agcggcaatg ccacggtgat tggctttgat ccaatcaaaa acgacggcgc
     8761 gctgcacgcc gtgctcggct atatgccgca gaaatttggt ctgtatgaag atctcacggt
     8821 gatggagaac ctcaatctgt acgcggattt gcgcagcgtc accggcgagg cacgtaagca
     8881 aacttttgct cgcctgctgg agtttacgtc tcttgggccg tttaccggac gcctggcggg
     8941 caagctctcc ggtgggatga aacaaaaact cggtctggcc tgtaccctgg tgggcgaacc
     9001 gaaagtgttg ctgctcgatg aacccggcgt cggcgttgac cctatctcac ggcgcgaact
     9061 gtggcagatg gtgcatgagc tggcaggcga agggatgtta atcctctgga gtacctcgta
     9121 tctcgacgaa gccgagcagt gccgtgacgt gttactgatg aacgaaggcg agttgctgta
     9181 tcagggggaa ccaaaagccc tgacacaaac catggccgga cgcagttttc tgatgaccag
     9241 tccacacgag ggcaaccgca aactattgca acgcgccttg aaactgccac aggtcagcga
     9301 cggcatgatt caggggaaat cggtacgtct gatcctcaaa aaagaagcca caccagacga
     9361 tattcgccat gccgacggga tgccggaaat caacatcaac gaaactacgc cgcgttttga
     9421 agatgcattt attgatttgc tgggtggtgc cggaacctca gaatcgccgc tgggcgcaat
     9481 attacatacg gtagaaggca cacccggcga gacggtgatc gaagcgaaag aactgaccaa
     9541 gaaatttggt gattttgccg ccaccgatca cgtcaacttt gccgttaaac gtggggagat
     9601 ttttggtttg ctggggccaa acggcgcggg taaatcgacc acctttaaga tgatgtgcgg
     9661 tttgctggtg ccgacttccg gccaggcgct ggtgctgggg atggatctga aagagagttc
     9721 tggtaaagcg cgccagcatc tcggctatat ggcgcaaaag ttttcgctct acggtaacct
     9781 gacggtcgaa cagaatttac gctttttctc tggtgtgtat ggcttacgcg gtcgggcgca
     9841 gaacgaaaaa atctcccgta tgagcgaggc gttcggcctg aaaagtatcg cctcccacgc
     9901 caccgatgaa ctgccattag gttttaaaca gcggctggca ctggcctgct cgctgatgca
     9961 tgaaccggac attctgtttc tcgacgaacc gacgtccggc gttgatccga ttactcgccg
    10021 cgaattctgg ctacatatca acagcatggt agagaaaggc gtcacggtga tggtcaccac
    10081 ccactttatg gatgaagcag aatattgcga ccgcatcggc ctggtgtacc gtgggaaatt
    10141 aattgccagc ggcacgccgg atgatttgaa agcgcagtcg gccaacgacg agcaacccga
    10201 tcccaccatg gagcaagcct ttattcagtt gatccacgac tgggataagg agcatagcaa
    10261 tgagtaaccc gatcctgtcc tggcgtcgcg tacgggcgct gtgcgttaaa gagacgcggc
    10321 agatcgttcg cgatccgagt agctggctga ttgcggtagt gatcccgctg ctactgctgt
    10381 ttatttttgg ttacggcatt aacctcgact ccagcaagct gcgggtcggg attttactgg
    10441 aacagcgtag cgaagcggcg ctggatttca cccacaccat gaccggttcg ccctacatcg
    10501 acgccaccat cagcgataac cgtcaggaac tgatcgccaa aatgcaggcg gggaaaattc
    10561 gcggtctggt ggttattccg gtggattttg cggaacagat ggagcgcgcc aacgccaccg
    10621 caccgattca ggtgatcacc gacggcagtg agccgaatac cgctaacttt gtacaggggt
    10681 atgtcgaagg gatctggcag atctggcaaa tgcagcgagc ggaggacaac gggcagactt
    10741 ttgaaccgct tattgatgta caaacccgct actggtttaa cccggcggcg attagccagc
    10801 acttcattat ccccggtgcg gtgaccatta tcatgacggt catcggcgcg attctcacct
    10861 cgctggtggt ggcgcgagaa tgggaacgcg gcaccatgga ggctctgctc tctacggaga
    10921 ttacccgcac ggaactgctg ctgtgtaagc tgatccctta ttactttctc gggatgctgg
    10981 cgatgttgct gtgtatgctg gtgtcagtgt ttattctcgg cgtgccgtat cgcgggtcgc
    11041 tgctgattct gttttttatc tccagcctgt ttttactcag taccctgggg atggggctgc
    11101 tgatttccac gattacccgc aaccagttca atgccgctca ggtcgccctg aacgccgctt
    11161 ttctgccgtc gattatgctt tccggcttta tttttcagat cgacagtatg cccgcggtga
    11221 tccgcgcggt gacgtacatt attcccgctc gttatttcgt cagcaccctg caaagcctgt
    11281 tcctcgccgg gaatattcca gtggtgctgg tggtaaacgt gctgtttttg atcgcttcgg
    11341 cggtgatgtt tatcggcctg acgtggctga aaaccaaacg tcggctggat tagggagaag
    11401 agcatgtttc atcgcttatg gacgttaatc cgcaaagagt tgcagtcgct gctgcgcgaa
    11461 ccgcaaaccc gcgcgattct gattttacct gtgcttattc aggtgattct gttcccgttc
    11521 gccgccacgc tggaagtgac taacgccacc atcgccatct acgatgaaga taacggcgag
    11581 cattcggtgg agctgaccca acgttttgcc cgcgccagcg cctttactca tgtgctgctg
    11641 ctgaaaagcc cacaggagat ccgcccaacc atcgacacac aaaaggcgtt actactggtg
    11701 cgtttcccgg ctgacttctc gcgcaaactg gataccttcc agaccgcgcc gttgcagttg
    11761 atcctcgacg ggcgtaactc caacagtgcg caaattgccg ccaactacct gcaacagatc
    11821 gtcaaaaatt atcagcagga gctgctggaa ggaaaaccga aacctaacaa cagcgagctg
    11881 gtggtacgca actggtataa cccgaatctt gactacaaat ggtttgtggt gccgtcgctg
    11941 atcgccatga tcaccactat cggcgtaatg atcgtcactt cactttccgt cgcccgcgaa
    12001 cgtgaacaag gtacgctcga tcagctactg gtttcgccgc tcaccacctg gcagatcttc
    12061 atcggcaaag ccgtaccggc gttaattgtc gccaccttcc aggccaccat tgtgctggcg
    12121 attggtatct gggcgtatca aatccccttc gccggatcgc tggcgctgtt ctactttacg
    12181 atggtgattt acggtttatc gctggtggga ttcggtctgt tgatttcatc actctgctcg
    12241 acacaacagc aggcgtttat cggcgtgttt gtttttatga tgcccgccat tctcctttcc
    12301 ggttacgttt ctccggtgga aaacatgcca gtatggctgc aaaacctgac gtggattaac
    12361 cctattcgcc actttacgga cattaccaag cagatttatt tgaaggatgc gagtctggat
    12421 attgtgtgga atagtttgtg gccgctactg gtgataacgg ccacgacagg gtcagcggcg
    12481 tacgcgatgt ttagacgtaa ggtgatgtaa cttcttatct ttcgccagca aagacactac
    12541 cgccgggccg gcaaggattg ccagccctgc aattgccagc aaaaagttgt tttccagtac
    12601 acgggacaac agccacagca caatcagcgc tgcgccaaaa taccaggtcg tggtcagctc
    12661 ttccagcacg tctgccacct cacgccagcg ttgctcgggg tggcgctgaa acaccaacat
    12721 catcactacc gtgacacaat acagtgcgca taatcccacg ccgacgtgaa ccagagtctt
    12781 actcatccag cccaccacca gcagcgccac taccagtaaa tgcaacataa tctgccagca
    12841 acttagacca gttgcgacac gaacacgttg ttgccacttc atggcttctc tcctgaagaa
    12901 tttatctcta cttactcaga gtaccgaact ttacggaaaa ttccgtaaga ccgcaccttt
    12961 ttgtgatgcc gactacacta tttcaatgag ataagaggca aagacaggaa aattatgccc
    13021 gatcaaacac aacaattttc gttcaaggtg ctcaccatca acattcacaa aggttttacc
    13081 gcgtttaacc gacgcttcat tttgccggaa cttcgcgacg cggtgcgtac cgtcagcgcc
    13141 gatattgttt gcctgcagga agtgatgggc gcgcacgaag ttcatccgct gcatgtggaa
    13201 aactggcccg atacctcgca ctacgagttt ctcgccgaca cgatgtggag cgattttgcc
    13261 tacggtcgca atgccgtata cccgaaaggg catcacggca acgccgtact gtcgcgttat
    13321 cccattgaac attatgagaa tcgcgatgtt tcggtcgatg gtgcggaaaa gcgcggcgtg
    13381 ctctactgcc gcattgtgcc accgatgacc ggaaaagcga ttcatgtgat gtgcgtacat
    13441 ctgggcctgc gtgaggcgca ccgtcaggcg cagcttgcga tgctcgccga atgggtgaat
    13501 gagctaccgg acggcgaacc ggtattggtg gcgggtgatt tcaatgactg gcggcaaaaa
    13561 gctaatcatc cgttaaaagt gcaggccgga ctggatgaga tttttacccg cgcccacgga
    13621 cgcccggcgc gcacgtttcc ggtgcaattt cctctactac gactggacag gatctacgtc
    13681 aaaaatgcca gcgccagcgc gccaaccgcg ttgccgctgc ggacatggcg acacctttct
    13741 gatcatgccc ctttaagtgc ggagattcat ttatgaaatg tagctggcgc gaaggcaata
    13801 agatccagtt gctggaaaac ggcgagcaat attatcccgc ggtgtttaag gcgattggcg
    13861 aggcacaaga acgcatcatt cttgaaacgt ttatctggtt tgaggatgac gtcggcaaac
    13921 agctgcatgc ggcactactg gcagcagcgc aacgcggggt taaagcggaa gtcttgctgg
    13981 atggctacgg ttcgccggat ctcagcgatg agtttgtcaa tgaactgacg gcagctggcg
    14041 tggtatttcg ctactacgat ccccgccctc gcctttttgg tatgcgcacc aatgtgtttc
    14101 gccggatgca tcgcaaaatt gtggtgatcg acgcgcgtat cgcctttatt ggcgggctga
    14161 attactccgc cgagcatatg tccagctacg gtccagaggc taaacaggat tacgcggtac
    14221 gccttgaagg gccgattgtc gaagatattc tccagtttga gctggaaaac ctgcctggac
    14281 agagcgcggc acgacgctgg tggcgacgtc atcacaaagc ggaagagaac cgccagccgg
    14341 gagaagcgca ggtattgctg gtctggcgcg ataacgaaga acatcgcgat gatattgaac
    14401 gtcactatct gaaaatgctc actcaggcgc ggcgagaagt cattatcgcc aacgcctact
    14461 tcttccccgg ctatcgattt ttacacgcct tgcgtaaagc ggcacggcgc ggggtgcgga
    14521 tcaaactgat cattcagggc gaaccggata tgccgattgt cagagtcggt gcgcgcttgc
    14581 tgtataacta tctggttaaa ggcggcgttc aggtatttga gtaccgccgc cgcccgctcc
    14641 acggcaaagt ggcattgatg gacgatcact gggcgacagt agggtccagt aatctcgatc
    14701 cgctcagttt gtcactgaat ctcgaagcaa atgtcatcat ccacgatcgt cattttaacc
    14761 agacgctgcg cgataatctg aacggcatta ttgccgcaga ttgtcagcag gtcgatgaga
    14821 ccatgctgcc gaaacgcacc tggtggaacc tgaccaaaag cgtgctggcg ttccactttt
    14881 tacgccactt cccggcgctg gttggctggc ttccggcaca cacaccacgt ctggcgcagg
    14941 ttgatccgcc cgcacaaccg acaatggaaa cgcaggatcg ggtagaaact gaaaacacgg
    15001 gggtaaaacc ctgatgagta aatcacaccc gcgctggcgc ttagcaaaga agatcctcac
    15061 ctggctgttt tttatcgcgg tgattgtgtt gctggtggtc tacgccaaaa aagtggactg
    15121 ggaagaggtc tggaaggtca tccgcgacta caatcgcgtt gcgctgctta gtgcggtcgg
    15181 gctggtggtc gtcagctatc tgatttacgg ctgctatgac ctgctcgccc gcttttactg
    15241 cggtcacaaa ctggcgaagc gccaggtgat gctggtgtcg tttatctgct acgccttcaa
    15301 cctgacgctc agtacctggg tcggcggcat tggtatgcgc tatcgtttgt actcccgact
    15361 agggttaccg ggcagcacta ttacgcggat tttctcgctc agtattacca ccaactggct
    15421 gggctatatt ttactggcag ggattatctt taccgcaggc gtggtggagt tgccggatca
    15481 ctggtatgtc gatcaaacta cgctgcgtat tctcggcatt ggcttactga tgattatcgc
    15541 ggtttatttg tggttttgcg ctttcgcgaa gcaccgccat atgaccatca aagggcaaaa
    15601 gctggtgctg ccttcatgga aattcgccct cgctcagatg ctgatttcca gcgttaactg
    15661 gatggtaatg ggggcgatta tctggctgtt acttggtcaa agcgtgaact atttctttgt
    15721 actgggcgtg ttactggtta gtagtattgc tggcgtcatc gtgcatattc cggtggggat
    15781 cggtgtgctg gaagcggtgt ttatcgcact actggctggg gagcatacct cgaaagggag
    15841 cattatcgcc gccctgctcg cttatcgcgt cctgtattac tttatcccgc tgctgctggc
    15901 gctgatctgt tacctgctac tggaaagcca ggcgaagaag ctacgggcga aaaatgaagc
    15961 ggcgatgtga agtttagtta agttctttag tatctgcatt tacgactaat gaaaaaaacg
    16021 cgtatgcctt tgccagacaa gcgttatagc tatcgcggca agtatcccaa agaagccgat
    16081 aaaatagtaa tagagtgtta atgcacctgt aacagcgcaa cgaaccggac tccacaactc
    16141 ttcactatgg catttccccg ccaattcaag gagcttattt cgtccatgcg agataatgcc
    16201 tgaccataac acaatcatta agcaacttat tacccaaacg ggtcgttcac tcattaatgc
    16261 ggcattcatg atgatcacca gggccaaccc tgtcaacgtc atcataatga taaaacggtg
    16321 agaaccagga tcaacattaa ataataaacc ggccagcata ctgattaaaa acatcgcgcc
    16381 cgcagtgctg aacacgacga taatggcagg tacaggagct aaacttgcaa cgacaatcgc
    16441 caccaaagag agtaaaaaca gatatattac gcctgcgatg acggcattta tcttgttgat
    16501 atccttactt aagccataca tacaaactaa taataaccct atggcgacat acattgttgg
    16561 tgaatcatat ttggttgccg taatttcgag atacttagct ccccaggcca gtcctgcggt
    16621 tgccaccaaa cctaaagcgg taatgagcca tataccgttt aatatcctgg tctcgtgctg
    16681 aaaatctaat ttattgctgt tttgtgagt
//
LOCUS       DAZFJF010000102        16606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFJF010000102 DAZFJF010000000
VERSION     DAZFJF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.102"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..2132
                     /gene="paaZ"
                     /locus_tag="ACHKAU_003646"
     CDS             87..2132
                     /gene="paaZ"
                     /locus_tag="ACHKAU_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO5532633.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            2380..4653
                     /gene="tynA"
                     /locus_tag="ACHKAU_003647"
     CDS             2380..4653
                     /gene="tynA"
                     /locus_tag="ACHKAU_003647"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO5532634.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(4711..6210)
                     /gene="feaB"
                     /locus_tag="ACHKAU_003648"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(4711..6210)
                     /gene="feaB"
                     /locus_tag="ACHKAU_003648"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO5532635.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            6446..7351
                     /gene="feaR"
                     /locus_tag="ACHKAU_003649"
     CDS             6446..7351
                     /gene="feaR"
                     /locus_tag="ACHKAU_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO5532636.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(7523..7852)
                     /locus_tag="ACHKAU_003650"
     CDS             complement(7523..7852)
                     /locus_tag="ACHKAU_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO5532637.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(7857..8042)
                     /locus_tag="ACHKAU_003651"
     CDS             complement(7857..8042)
                     /locus_tag="ACHKAU_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO5532638.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(8039..10678)
                     /locus_tag="ACHKAU_003652"
     CDS             complement(8039..10678)
                     /locus_tag="ACHKAU_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO5532639.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            10886..11875
                     /gene="ldhA"
                     /locus_tag="ACHKAU_003653"
     CDS             10886..11875
                     /gene="ldhA"
                     /locus_tag="ACHKAU_003653"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5532640.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            11986..12408
                     /gene="hslJ"
                     /locus_tag="ACHKAU_003654"
     CDS             11986..12408
                     /gene="hslJ"
                     /locus_tag="ACHKAU_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO5532641.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(12405..12671)
                     /locus_tag="ACHKAU_003655"
     CDS             complement(12405..12671)
                     /locus_tag="ACHKAU_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO5532642.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            12945..16469
                     /gene="nifJ"
                     /locus_tag="ACHKAU_003656"
     CDS             12945..16469
                     /gene="nifJ"
                     /locus_tag="ACHKAU_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO5532643.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         4143 a         4285 c         4166 g         4012 t
ORIGIN      
        1 taacatttcg ttaaaagatc ccttgctttt tatgattcgc gatttaacta ttagcaacag
       61 aaatgtgaaa catctggaga gtagcgatgc agcagttagc cagtttctta tccggtacct
      121 ggcagtctgg ccggggccgt agccgtttga ttcaccacgc tattagcggc gaggcgttat
      181 gggaagtgac cagtgaaggt cttgatatgg cggctgcccg ccagtttgcc attgaaaaag
      241 gtgcccccgc cctccgcgcg atgaccttta tcgaacgtgc ggcgatgctt aaagcggtcg
      301 ctaaacatct gctgagtgaa aaagagcgtt tctatgctct ttctgcgcaa acaggcgcaa
      361 cgcgggcaga cagttgggtt gatattgaag gtggcattgg gacgttattt acttacgcca
      421 gcctcggtag ccgggagctg cctgacgata cgctgtggcc ggaagatgaa ttgatcccct
      481 tatcgaaaga aggtggattt gccgcgcgcc atgtactgac ctcgaagtca ggcgtggcag
      541 tgcatattaa cgcctttaac ttcccctgct ggggaatgct ggaaaagctg gcaccaacgt
      601 ggctgggcgg aatgccagcc atcatcaaac cagctaccgc gacggcccaa ctgactcagg
      661 cgatggtgaa atcaattgtc gatagtggtc ttgttcccga aggcgcaatt agtctgatct
      721 gcggtagtgc tggcgacttg ttggatcatc tggacagcca ggatgtggtg actttcacgg
      781 ggtcagcggc gaccggacag atgctgcgag ttcagccaaa tatcgtcgcc aaatctatcc
      841 ccttcactat ggaagctgat tccctgaact gctgcgtact gggcgaagat gtcaccccgg
      901 atcaaccgga gtttgcgctg tttattcgtg aagttgtgcg tgagatgacc acaaaagccg
      961 ggcaaaaatg tacggcaatc cggcggatta ttgtgccgca ggcattggtt aatgctgtca
     1021 gtgatgctct ggttgcgcga ttacagaaag tcgtggtcgg tgatcctgct caggaaggcg
     1081 tgaaaatggg cgcactggta aatgctgagc agcgtgccga tgtgcaggaa aaagtgaaca
     1141 tattgctggc tgcaggatgc gagattcgcc tcggtggtca ggcggattta tctgctgcgg
     1201 gtgccttctt cccgccaacc ttattgtact gtccgcagcc ggatgaaaca ccggcggtac
     1261 atgcaacaga agcctttggc cctgtcgcaa cgctaatgcc agcacaaaac cagcaacatg
     1321 ctctgcaact ggcttgtgca ggcggcggta gccttgcggg aacgctggtg acggctgatc
     1381 cgcaaattgc gcgtcagttt attgccgacg cggcacgtac gcatgggcga attcagatcc
     1441 tcaatgaaga gtcggcaaaa gaatccaccg ggcatggctc cccactgcca caactggtac
     1501 atggtgggcc tggtcgcgca ggaggcggtg aagaattagg tggtttacga gcggtgaaac
     1561 attacatgca gcgaaccgct gttcagggta gtccgacgat gcttgcagct atcagtaaac
     1621 agtgggtgcg cggtgcgaaa gtcgaagaag atcgtattca tccgttccgc aaatattttg
     1681 aggagctaca accaggcgac agcctgttga ctccccgccg cacaatgaca gaggccgata
     1741 ttgttaactt tgcttgcctc agcggcgatc atttctatgc acatatggat aagattgctg
     1801 ctgccgaatc tattttcggt gagcgggtgg tgcatgggta ttttgtgctt tctgcggctg
     1861 cgggtctgtt tgtcgatgcc ggtgttggtc cggtcattgc taactacggg ctggaaagct
     1921 tgcgttttat cgaacccgta aagccaggcg ataccatcca ggtgcgtctc acctgtaagc
     1981 gcaagacgct gaaaaaacag cgtagcgcag aagaaaaacc aacaggtgtg gtggaatggg
     2041 cggtagaggt attcaatcag catcaaaccc cggtggcgct gtattcaatt ctgacgctgg
     2101 tggccaggca gcacggtgat tttgtcgatt aatcggtgaa tgaagggcaa cggccaatag
     2161 ttgccctttt atttcactaa gttttgtgac gttgtcacat tatgcatgat gtgtacatct
     2221 attttcaggg cacccactgt atgaaaagct ggcacacctg ccaaacaacc tggcaggtgc
     2281 aggcaatccc ctttgcatca gtactgataa tgtgaacctg actaaaccgc ccacagagcg
     2341 cggttgctaa caagaacaca acatctgacg aggttaataa tgggaagccc ctctctgtat
     2401 tctgcccgta aaacaaccct ggcgttggca gtcgccttaa gtttcgcctg gcaagcgccg
     2461 gtatttgccc acggtggtga agcgcatatg gtgccaatgg atgaaacgct taaagaattt
     2521 ggtgccgatg tgcagtggga cgactacgcc cagatcttta ccctgattaa agatggcgcg
     2581 tacgtgaaag tgaagcctgg tgcgcaaaca gcaattgtta atggtcaacc tctggcactg
     2641 caagtgccgg tagtgatgaa agacaataag gcctgggttt ctgacacctt tattaacgat
     2701 gttttccagt ccggtctgga tcaaaccttt caggtagaaa agcgccctca cccacttaat
     2761 gcgctaactg cggacgaaat taaacaggcc gttgaaattg ttaaagcttc cgcggacttc
     2821 aaacccaata cccgttttac tgagatctcc ctgctaccgc cagataaaga agctgtctgg
     2881 gcgtttgcgc tggaaaacaa accggttgac cagccacgca aagccgacgt cattatgctc
     2941 gacggcaaac atatcatcga agcggtggtg gatctgcaaa acaacaaact gctctcctgg
     3001 caacccatta aagacgccca cggtatggtg ttgctggatg atttcgccag tgtgcagaac
     3061 attattaaca acagtgaaga atttgccgct gccgtgaaga aacgcggtat tactgatgcg
     3121 aaaaaagtga ttaccacgcc gctgaccgta ggttatttcg atggtaaaga tggcctgaaa
     3181 caagatgccc ggttgctcaa agtcatcagc tatcttgatg tcggtgatgg caactactgg
     3241 gcacatccca tcgaaaacct ggtggcggtc gttgatttag aacagaaaaa aatcgttaag
     3301 attgaagaag gtccggtagt tccggtgcca atgaccgcac gcccatttga tggccgtgac
     3361 cgcgttgctc cggcagttaa gcctatgcaa atcattgagc ctgaaggtaa aaattacacc
     3421 attactggcg atatgattca ctggcggaac tgggattttc acctcagcat gaactctcgc
     3481 gtcgggccga tgatctccac cgtgacttat aacgacaatg gcaccaaacg caaagtcatg
     3541 tacgaaggtt ctctcggcgg catgattgtg ccttacggtg atcctgatat tggctggtac
     3601 tttaaagcgt atctggactc tggtgactac ggtatgggca cgctaacctc accaattgct
     3661 cgtggtaaag atgccccgtc taacgcagtg ctccttaatg aaaccatcgc cgactacact
     3721 ggcgtgccga tggagatccc tcgcgctatc gcggtatttg aacgttatgc cgggcctgag
     3781 tataagcatc aggaaatggg ccagcccaac gtcagtaccg aacgccggga gttagtggtg
     3841 cgctggatca gtacagtggg taactatgac tacatttttg actggatctt ccatgaaaac
     3901 ggcactattg gcattgatgc cggtgctacg ggcatcgaag cggtgaaagg tgttaaagcg
     3961 aaaaccatgc acgatgagac ggcgaaagat gacacgcgct acggcacgct tatcgatcac
     4021 aatatcgtgg gtactacaca ccaacatatt tataatttcc gcctcgatct ggatgtagat
     4081 ggcgagaata acagcctggt ggcgatggac ccagtggtaa aaccgaatac tgccggtggc
     4141 ccacgcacca gtaccatgca agttaatcag tacaacatcg gcaatgaaca ggatgccgcg
     4201 cagaagtttg atccgggcac gattcgtctg ttgagtaacc cgaacaaaga gaaccgcatg
     4261 ggcaatccag tttcctatca aattattcct tatgcaggtg gtactcaccc ggtagcaaaa
     4321 ggtgcccagt tcgcgccgga cgagtggatc tatcatcgtt taagctttat ggacaagcag
     4381 ctttgggtaa cgcgttatca tcctggcgag cgtttcccgg aaggcaaata tccgaaccgt
     4441 tctactcatg acaccggtct tggacaatac agtaaagata acgagtcgct ggacaacacc
     4501 gacgccgttg tctggatgac caccggcacc acacatgtgg cccgcgccga agagtggccg
     4561 attatgccga ccgaatgggt acatactctg cttaaaccat ggaacttctt tgacgaaacg
     4621 ccaacgctag gggcgctgaa gaaagataag tgattgtttc agacaaaaaa acggcaccag
     4681 gtgccgtttt ttacttatga gcgaaccaga ttaataccgt acacacaccg acttagtttc
     4741 acaccaaccg tccagccagt cggggccaaa atcacggccc gttcctgact gcttcatccc
     4801 accaaacggt aagttagcgt caattaaggt atggctgttt acccacaccg tccctgcctg
     4861 taagcgatcg ctatattcca gagcctggga gagattttgc gtccagacac tggcagttaa
     4921 gccatattcc gtgtcgtttg ccagttgtaa cgcctcttct ccatccgcta ctcgcaccag
     4981 gtttaccacc ggaccaaaca cctcttcacg agttaagcgc aatttagcat cgggatttac
     5041 caccagcgtt ggcgcaacat aatacccctc tccggctggt ccattcgacc cgcgaatcag
     5101 ctctgcttgc tgtgcctgcg catcgtcgag gaatgaacac actttgtcgc agtgcgcacg
     5161 agaaaccaaa gggttaatct gtgcaacagg tgacatcccc ggtcccactt gcaacgattt
     5221 taccgcctgc tcaaatccac taaccagcgt gtcaaacaac ggcgcttcaa tataaattcg
     5281 cgaactggcg gcgcatactt gcccttgatt caggaagctt ccggtcatca agccttcaat
     5341 aacccattgc ggatcagcat cttttaatac aattgccggg tttttaccgc ccagttccag
     5401 cgttacacgc gttaagtgat cagcagcagt tctggcaata ccttttcccg tcgcggttga
     5461 accggtaaaa ctgattttcg caacatgagg atgtgacgtc agggccgcgc cgcatacagc
     5521 acctgacccg gtgacgacat taaaaacgcc atcagggata ccagcctcgc tggccagttc
     5581 cgccacgcgc aacatcgtca gtggcgtggt ttccgaaggc ttaatcacga ttgaacagcc
     5641 tgctgccagt gctggcatca ccttccacat accaatcatc aacggaaagt tccatggcac
     5701 aattcccgcc actacgccaa ccggctcttt acgcgtccag gcctgataac gcgccccctg
     5761 gggtaaggga atcgacaagt ccagcgtttt acccgcgatt ttggtcgtta acccggcggt
     5821 ataacgcatc cagttcagcg tacagcccac ttcaaaagca cgagaaatgg caattgactt
     5881 gccttgctcc agggtttcca gttgcgccag ctcctcactg tgctgctcca ccagatcagc
     5941 aaaacgtagc agaatacgtt cacgctctgc gggtaatcgc ccggcccagc gacgcgagac
     6001 aaaggcccgc caggcagaca tgactgcgtt atctacatcc gcttcgttgg catcagcagt
     6061 agacgcaatt tcttgcccgg tggccggatc aaagatcgcc aatcgttttt cactttgtgc
     6121 ggggcctgga cgaccatcaa tataaagacc gtgttgacga tcgagaaact gttggacctg
     6181 gcttaatact gctacatgcg gctctgtcat cacttttcct tattatttac ccagtgtgat
     6241 ttcagtgtac gcagcgacaa gaaaagtcac ttttttcgct gtgacattcg ctttgtcagc
     6301 tgtgacaagc tccgcaaatc gtgacaataa ccgcttatgt ctctatgcct gagaagactt
     6361 atttcctata gtcaggctca ttagcttcgt ttactgcaac acaaatgcaa caataaaaat
     6421 acatttcaca gagcgaaaac gtgccatgaa ccccgcagtg gataatgagt ttcagcaatg
     6481 gctttcccaa atcaatcagg tatgcggaaa ttttaccgga cacctgctga ctgagcgtta
     6541 cacgggtgta ctggatacgc attttgccaa aggactaaag ctgagtaccg tgacaaccag
     6601 cggggtgaat ttatcccgca cctggcagga agtaaaaggc agcgacgatg cctggtttta
     6661 caccgttttt cagcttagtg gtcaggcaat aatggagcag gatgagcgtc aggtgcagat
     6721 tggcgctggc gatattacgt tactcgatgc ctcacgcccc tgttcgcttt actggcagga
     6781 gtcttctaaa cagatttcat tacttttgcc acgcactctg ctggaacaat attttcccca
     6841 tcaaaaacct gtctgcgcag aaagactgga cgctgactta cccatggtgc aactcagtca
     6901 tcgcctgtta caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc
     6961 gctacaggcg atggtgtgtc tgctgcgccc ggtacttcat cagcgggaat ctgttcaacc
     7021 tcgtcgtgaa cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga
     7081 gatattacgc ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg
     7141 aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt
     7201 ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca
     7261 ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct ttgggatgac
     7321 gccaggcgag tatcgacgta aattccgcta actaattggc gttgtgtgta ctgcaacgcc
     7381 aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga
     7441 gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta
     7501 tcccgatatt gcggttttgc tattaaaatt ttcgcaccca tttaccgttt atcccttgta
     7561 catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat
     7621 ctacagacac attattttgc tttgccagtt gttggtaact cgctttacgt tcggcattaa
     7681 tgtcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa
     7741 atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc
     7801 tgcttaccag cccaacaaga aacgcacaaa aaagtaatgt tttcttcatc atcgcctcag
     7861 aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc
     7921 tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttgggcgc agcgacttca
     7981 atacgcggcg tacaggcagc cagcataaaa gatgacgtca acgcagccag taaaattttc
     8041 attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct
     8101 gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat
     8161 tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac
     8221 ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg
     8281 accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt
     8341 tcaccaccgc atctgcggtg tctttatcca gccgcaaggt cattggcccg ctatttgcca
     8401 gccaaccatc tttcactatc catttttcgc tgtttagcca taacggcaat gcaccactaa
     8461 atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga
     8521 gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagta
     8581 cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc
     8641 acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt
     8701 cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac
     8761 ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc
     8821 ctttcaacac gccgtgtcct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa
     8881 cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct
     8941 ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc
     9001 agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct ttaaagagga
     9061 aatacgtcgg atcgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc
     9121 cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag gagggatgct
     9181 gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca
     9241 tcgtaccgta ttgtaactgg tcgaaaccgg tggagaggtc ggtcagcgta atcgtgctgt
     9301 catgccattc accttttcct gcgacatccc aggtggcatt catcggtgta aaactgcctt
     9361 ttccccagta gcgccactgc cagcggcccg cgtcagggag aaaatcattc gccagcccat
     9421 ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac
     9481 gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga
     9541 tgtccagcga atcgatgact cttcccttcg aacgtaataa cgcgcctggc tcaaaggcca
     9601 gcgttgggtc agtcagactt ccacttagct gcgcgggtaa gcgggcatat aaaatgaggt
     9661 ccgcctgttt cgcttcaccg gtcagttgca gaggcagctg gctgttatcc atgcttaatt
     9721 ttcctgggcc aaaatttagc accgcgttgc ctttacccgc ttgcccctgg gtcagcacac
     9781 tcagtcgtcc gctgatcaga gcgttctcaa gccctgcctg ccagttgtcg actttgacac
     9841 ccagtcggcc actcaaagga aaacctgcat acggccaact ccagcgacca tcgcttacgg
     9901 tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg
     9961 ccagcacaat caattgcccg ctattttcct gccagtccag ctctgcatcc accagtgacg
    10021 gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca
    10081 cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga
    10141 catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt
    10201 cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc
    10261 attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg
    10321 actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg
    10381 ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg
    10441 aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg
    10501 gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga
    10561 gcggtagcca gatgcccgcc agagtgggaa cccacagccc gagcgtcatc aacagcgtca
    10621 acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg
    10681 gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt
    10741 taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata
    10801 tcgccatagc tttcaattaa atttgaaatt ttgtaaaata tttttagtag cttaaatgtg
    10861 attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga
    10921 caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt
    10981 tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt
    11041 aaacgatgac ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat
    11101 cgccctgcgc tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct
    11161 gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat
    11221 gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt
    11281 ctctctggaa ggtctgaccg gatttactat gtatggcaaa acggcaggcg ttatcggtac
    11341 cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc
    11401 gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc
    11461 aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta
    11521 ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac
    11581 cagtcgcggt gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat
    11641 tggttcgttg ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagacaaatc
    11701 caacgacgtg atccaggatg acgtgttccg tcgtctgtct gcctgccaca acgtgctgtt
    11761 taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct
    11821 gcaaaactta agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatcttg
    11881 ccgctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct
    11941 attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaactagcc
    12001 gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt
    12061 acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg
    12121 accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc
    12181 atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg
    12241 ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt
    12301 agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg
    12361 accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag
    12421 ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca
    12481 atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt
    12541 gcttctccac tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc
    12601 tgctgaacag gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac
    12661 gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca taaataatag
    12721 gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt
    12781 atggctattt ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac
    12841 aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc
    12901 agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg
    12961 gtaatggcgc ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta
    13021 ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga
    13081 acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta
    13141 ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt
    13201 tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actcacaccg tttgtcctgc
    13261 atgtagcggc acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg
    13321 tcatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggcaaacgtc caggaagcgc
    13381 aagactttgc tctcatttcg cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt
    13441 tctttgatgg tttccgcacg tcccacgaaa tcaacaaaat tgtcccgctg gccgatgaca
    13501 cgattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcactcaacc
    13561 cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg
    13621 aagccaccaa cccatggtac gacgcggtct atgaccatgt tgaacaggcg atgaatgatt
    13681 tctctgccgc gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg
    13741 aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat
    13801 tgctaacccg tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc cccttctcag
    13861 ccaaacactt gctacaaact ctaccgggat ccgtacgtag cgtggcggta ctggacagaa
    13921 ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag
    13981 aagcctttaa taatggcaag cgcgaaactc tcccccgtgt cattggtggg cgctatggtc
    14041 tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg
    14101 ctaaaccgaa agcgcgcttt acggttggta tttacgatga tgtaaccaat ctgtccctgc
    14161 cgttgccgga aaacacccag ccaaactcgg cgaaacttga agccttgttt tatggccttg
    14221 gtagtgatgg cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc
    14281 cgtggtacgc acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt
    14341 ctcaccttcg agtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt
    14401 ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac
    14461 ctggcggcat tttcctgctc aacacaccgt acagcgcaga tgaagtgtgg tcgcgcttgc
    14521 cgcaagaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg attaacgcgg
    14581 cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt
    14641 ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga
    14701 ttgccaaaag ttacagtagc aaaggccagg atctggtgga acgcaattgg caggctctgg
    14761 cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca
    14821 atcgaccgcc agtggtttcc gatgccgctc ctgatttcgt gaaaaccgta accgctgcga
    14881 tgctcgccgg gcttggtgac gccctccccg tttcggcgct gccgccagac ggctcctggc
    14941 cgatgggcac tacgcgctgg gaaaaacgta atattgccga agagatcccc acctggaaag
    15001 aggaactctg tacccaatgt aaccactgcg ttgccgcttg cccacactca gctattcgcg
    15061 caaaagtggt gccgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg
    15121 tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg gaagattgca
    15181 ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta
    15241 aagccatcaa tatgatgtct cgcctggaac atgtcgaaga agagaaaatc aattacgatt
    15301 tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat
    15361 cgcagctgat tacaccgctg tttgaatatt caggtgcttg ctccggttgt ggcgagacgc
    15421 cgtatattaa attgctgact cagctctatg gcgaccggat gttgatcgct aacgccaccg
    15481 gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg
    15541 gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt
    15601 tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg
    15661 ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc
    15721 gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc
    15781 tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc gacggctggg
    15841 cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca
    15901 ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac
    15961 cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt aaagatcttg
    16021 gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc
    16081 tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca
    16141 ttgcttatag cccgtgtgaa gagcatggtt acgatctggc actcagccat gaccagatgc
    16201 gccaactcac agctaccggc ttctggccgc tatatcgctt cgatcctcgt cgtgccgatg
    16261 aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt
    16321 tacttcacga gcaacgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt
    16381 tatggaaaga tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg
    16441 gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa
    16501 aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat
    16561 gcaatggccc aacagaaaat aaaatctgtg acaataaagg catata
//
LOCUS       DAZFJF010000103        16549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFJF010000103 DAZFJF010000000
VERSION     DAZFJF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.103"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..203)
                     /locus_tag="ACHKAU_003657"
     CDS             complement(51..203)
                     /locus_tag="ACHKAU_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5532644.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(468..1202)
                     /gene="cysH"
                     /locus_tag="ACHKAU_003658"
     CDS             complement(468..1202)
                     /gene="cysH"
                     /locus_tag="ACHKAU_003658"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5532645.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1276..2988)
                     /gene="cysI"
                     /locus_tag="ACHKAU_003659"
     CDS             complement(1276..2988)
                     /gene="cysI"
                     /locus_tag="ACHKAU_003659"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO5532646.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2988..4787)
                     /gene="cysJ"
                     /locus_tag="ACHKAU_003660"
     CDS             complement(2988..4787)
                     /gene="cysJ"
                     /locus_tag="ACHKAU_003660"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO5532647.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5106..5468
                     /gene="queD"
                     /locus_tag="ACHKAU_003661"
     CDS             5106..5468
                     /gene="queD"
                     /locus_tag="ACHKAU_003661"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO5532648.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5546..6817
                     /gene="ygcN"
                     /locus_tag="ACHKAU_003662"
     CDS             5546..6817
                     /gene="ygcN"
                     /locus_tag="ACHKAU_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5532649.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6808..7068
                     /gene="ygcO"
                     /locus_tag="ACHKAU_003663"
     CDS             6808..7068
                     /gene="ygcO"
                     /locus_tag="ACHKAU_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5532650.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7085..7660
                     /gene="ygcP"
                     /locus_tag="ACHKAU_003664"
     CDS             7085..7660
                     /gene="ygcP"
                     /locus_tag="ACHKAU_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO5532651.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7808..8668)
                     /gene="ygcQ"
                     /locus_tag="ACHKAU_003665"
     CDS             complement(7808..8668)
                     /gene="ygcQ"
                     /locus_tag="ACHKAU_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5532652.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8665..9444)
                     /gene="ygcR"
                     /locus_tag="ACHKAU_003666"
     CDS             complement(8665..9444)
                     /gene="ygcR"
                     /locus_tag="ACHKAU_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO5532653.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9422..10831)
                     /gene="ygcS"
                     /locus_tag="ACHKAU_003667"
     CDS             complement(9422..10831)
                     /gene="ygcS"
                     /locus_tag="ACHKAU_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5532654.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10853..12307)
                     /gene="ygcU"
                     /locus_tag="ACHKAU_003668"
     CDS             complement(10853..12307)
                     /gene="ygcU"
                     /locus_tag="ACHKAU_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO5532655.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12377..13162)
                     /gene="ygcW"
                     /locus_tag="ACHKAU_003669"
                     /pseudo
     CDS             complement(12377..13162)
                     /gene="ygcW"
                     /locus_tag="ACHKAU_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13480..14757
                     /gene="yqcE"
                     /locus_tag="ACHKAU_003670"
     CDS             13480..14757
                     /gene="yqcE"
                     /locus_tag="ACHKAU_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO5532656.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14784..16262
                     /gene="ygcE"
                     /locus_tag="ACHKAU_003671"
     CDS             14784..16262
                     /gene="ygcE"
                     /locus_tag="ACHKAU_003671"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO5532657.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3930 a         4349 c         4171 g         4099 t
ORIGIN      
        1 agaccaaacc atcacaattc agtacgcggg gttttacccc cgcgtggtgg ctacttcttc
       61 gattcgtaag cgagaactgc cttaaactcc ataccacgtt ctctgatact caactcacac
      121 aacgagtcac ctaccatcag cgtgaatccc agtactgtac aacacagtac gatgattgcc
      181 acaagggcat attttgtcag catgtcttgc ctccctgcaa agaagagact aacattcgaa
      241 ttgtttgggt tcgaacgtaa gcctcaggtt gatagaaata tcgcctgggg cttttgtcca
      301 tctggaacct cgcgaatgct taacgccaga cagcctcaag cacccgacgc cattctatac
      361 ctgataattc ttccagcggc gttcacgttt aaaatcagga atactgctcg caaaataaaa
      421 acgcccccat catttctgac agaggcgttt aatttatccg gcaatattta cccttcgtgc
      481 aatccacatt ccctttttaa gccaaagaaa cgtgtttctt cttccgacat gccaggttcc
      541 catttacggg ttgtatgggt atcgcccacc gataaatatc cttcgtccca taatgggtga
      601 tatttcaggc catgtttttg cagatactga tatatcgttc ggttatccca gtcgataatc
      661 ggcagcactt taaatacgcc acgctgaatt gccagcaccg gtaaattggc gcggctgccg
      721 gattgttcgc ggcgcaggcc agcaaaccac gtttgcgcat tcagttcttt cagcgcccgg
      781 ttcatcggtt cgactttgtt gatgtcattg tacttttcaa tgccttcaac gccctgttcc
      841 cacagtttgc cgtagcgtgc ttcctgccag gcggcgcttt cggtagcgcg gtacactttc
      901 aggttgagct tgagtttgtc cgttaactcg tcaataaagc ggtaggtttc cgggaacaga
      961 tagccggtgt cggtgaggat caccggaata tccgggtgaa tctgattcac cagatgcagg
     1021 ctcaccgccg cctgaatgcc aaagctggaa gaaagtacat attcaccggg cagattatcc
     1081 agcgcccagg caacgcggcc ttcagcgtcc agtttttcca gttcggcgtt agtttctgcc
     1141 agcgccagaa tgcgatctac cttcggcagt tcgttcaggg cgtttagatc gagtttggac
     1201 atagattcct cactgtttgc cttgcctgat gcgacgctca cgcgtcttat caggcctaca
     1261 agaccgggct gatggttaat cccacaaatc gcgcgccgga tcgagcaccg ggcgaatgat
     1321 gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct tcgcgctctt tcgcccagcg
     1381 ccctatcagt tcatcaagcg acgccaggat ttccggctcg gtgatgtttt ctttatacat
     1441 ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc aggttgtagc gacccggcgc
     1501 tttacccacc aggcccactt ccgccagcat cgcgcgacca caaccgttcg ggcagcctgt
     1561 tacacgcatc acgatatgct catcgctgac accatgtttc gccattaaat tatcgatgtt
     1621 gtcgataaaa gacggcagga aacgctctgc ttccgccatc gccagcgggc aagtcgggaa
     1681 cgagacgcag gccatcgagt tttcacgctg cggcgtgacg gcattcatta acccgctctc
     1741 tttggcgatc ttctcgatct tcgctttctc gctttccggt acaccggcga tgatcagatt
     1801 ctggttcgcc gtaatgcgga aatcgccttt gtggatcttc gcgatctcca gcaggccggt
     1861 tttcagcgga cgccccggat aatcaaggat gcgaccattt tcgataaaca gcgtcaggtg
     1921 ccagttatca tcaatgccct taacccagcc aatacgatcg cctcgtccgg tgaactcata
     1981 tggacggatc ggttcaaatt tgatccccgc gcgacgctcc acttccgctt taaacgtctc
     2041 aacccccacg cgctccagcg tgtatttggt tttggcattt ttacgatcgg ttcggttacc
     2101 ccagtcacgc tgagttgtca cgacggcttc ggccaccgcc agcgtatgct ccagcggcag
     2161 atagccaaac tcgctcgccg tgcgggcgta ggttttcttg ttgccgtgtt cgatagaaag
     2221 cccaccgccc accagcaggt tgaagcctac cagcttgccg ttttcggcga ttgccacgaa
     2281 gttcatgtcg ttggcatgca gatcgatatc gttctgcggc gggatcacta ccgtggtttt
     2341 gaacttacgc ggcaggtagg tctgaccaag gatcggttct tcatcagtag tggcgacttt
     2401 ttcgtggtcg agccagatct ccgcatacgc gcgggtacga ggcaacagat gctcagaaat
     2461 cttcttcgcc cactcgtacg cttccgcgtg cagctgcgac tcgtaagggt tcgaggtgca
     2521 gagtacgtta cggttcatgt cgttagctgt cgccagcgca tcaagaccga ccgagtgcag
     2581 catctggtgc accggtttga cgttcttttt cagaatgccg tggaactgaa acgtctggcg
     2641 gttggtcagg cgaatgctgc catagatggt gttttcaccg gcaaatttgt cgatcgcctg
     2701 ccactgttta gtggtaatca ccccacccgg cagacgacag cgaagcagca tcgcgtggcg
     2761 cggctccagc ttctgttcag cacgttcggc gcggatgtcg cggtcatcct gctgatacat
     2821 gccgtggaag cggatcagca ggaagttgtc gcccttaaag ccgccggtca gaccgtcgtt
     2881 taaatcttcc gcaatggtgc cgcgcaggta gttgctttca agcttcatgc gctcggcgtc
     2941 tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt tcgctcatta gtagacatct
     3001 cgctgataac ggcgctctac gcgcagctca cttaaaaatt catccgccgc ttcggtgtcc
     3061 atgccaccaa attcggcaat cacttccaga agtgcctgct caacgtcttt cgccatgcga
     3121 ttagcgtcgc cgcagacata aatgtgggca ccatcattga tccagcgcca cagctccgcg
     3181 ccctgttcgc gcagtttgtc ttgtacgtaa actttttctt tttggtcgcg cgaccaggca
     3241 agatcgatac gtgtcagcac gccatctttg acgtagcgct gccactccac ctgatacagg
     3301 aagtcttccg taaagtgcgg attaccaaag aacagccagt ttttacctgg cgcttcgtcg
     3361 gcggcgcgtt gctgcataaa ggcgcggaac ggcgcaatac cggtgcctgg gccaatcata
     3421 atcaccgggg tttccgggtt agcgggcagg cgaaagttat cgttatgttc gataaatacg
     3481 cggacttcgc cctcttcttc cacgcgatcc gcgaggaagc tggaggcacc accggcacgg
     3541 gctcggcctt ccacgtcgta acgcaccaca ccaacggtga cgtgtacttc gttctcgact
     3601 tccgcctgcg aggaggcgat ggaatacagg cgcggggtca gcgggcgcag cagattaatc
     3661 agcgcttcgg catccagttg cgccggagag aaacgcacca tgtcgacaat cggcgtcgtc
     3721 gcggcgtaat gctgtaactt cgctttatcg cccaccagcg gcagcagtgt ttcgctgcgg
     3781 gtaagcgtgg cgtaattctc aacaatgttg gcggtgttga cggtcagttc gaagtgccac
     3841 tgtagcgctt cgttcagagg caacgttttg ccctcgacgg tgacaggttc atcgcctttc
     3901 agccacagca gttcgacaag ttctttcacc agtgccggat cgttctgata ccagacgccc
     3961 agcgcgtcac ccggctggta acgcaggccc gagtcaccta agtcaatttc gatatggcga
     4021 acgtcttttt cagagttacg cccggtaatt ttctggttaa cagagaggct agccaccagc
     4081 ggcgcgtctt tgctgtacgg gctggtgtgg atttcattta ccgcgccagt agcgacggat
     4141 tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat caaccacgcg ggcgcgccac
     4201 tcgctggcag cagcctggta ttcaacatcg gcatcgacac ggtcgagcag gcgttcacca
     4261 cccagttccg ccagcttgct gtcgaaatct ttcccggact ggcagaaaaa ttcataagag
     4321 ctatcgccga ggctaaacac ggcaaacgcg gtgttttcca gctttggcgc ttttttggag
     4381 aacaggaact tatgcagcgc gacggcttct tccggcggtt ccccttcccc ttgcgttgac
     4441 gtcactacga tgagcagttt ttcgctggcg atttgtttga atttatagtc gcccgcgttc
     4501 accagcttaa cgttcagttt tgctgctaat aaatcatcgc gtaatgcttc agcaacccgg
     4561 cgcgcattgc cggtttgcga ggcggagata atagttatac ccggcatttc tgcggctggc
     4621 gctggcgtcg ctgcaagcgc agcaggctgc tgattgagca cgccccagaa atagccagaa
     4681 acccaggcaa gctgggtggg agttaaatcg gtcgtggccg cctgaaggcg tgccagttgc
     4741 tccgggttca acggaagcaa cgcggaaggt gggacctgtg tcgtcatgcg tcgttatgtt
     4801 ccagtaagca aagctgtttc tgcgccctgt cagcgcccat aaaacagaag agaaggtaag
     4861 gttaacgggg tgaacggtgt ggattaaaga cgggatagcg ataactaata accaaatcga
     4921 ctaacctgtt ttagcaatag tctttaacaa caaaatagat taaccaacct aatgaaaaac
     4981 aaatgaattt agccaatcat taagataaat cagcgatttt gcgcaacaaa gtcgttttag
     5041 ataatgcgaa aaaacagcct ttccggtact ctacggcggt tttatcgtcc tgtagagaaa
     5101 ttatgatgtc caccacgtta tttaaagatt tcaccttcga agccgctcac cgcttaccac
     5161 acgtcccgga agggcataaa tgtggtcgcc tgcacgggca ttcctttatg gtgcgactgg
     5221 aaattaccgg ggaagtcgat ccgcatacgg gctggattat cgatttcgct gaactaaaag
     5281 cggcgtttaa accaacctac gagcgcctcg atcaccatta tctcaatgat attccaggtc
     5341 tggaaaaccc aaccagcgag gttttagcaa aatggatttg ggatcaggtt aaacccgttg
     5401 tgccgctgtt aagtgcggtg atggtaaaag aaacctgtac cgcaggttgt atctatcgcg
     5461 gcgaatgata agagtgtgtc ggcggtcaat ttcccttaag taacgctatg ttagggtgtt
     5521 gtgttctgga tatctggggc atgacatgga agacgactgc gacattatta ttattggtgc
     5581 cggtattgca ggcaccgctt gcgcgttacg ctgcgcgcga gcgggtttat ccgttttgtt
     5641 actggaacgc gctgaaatcc ccggcagcaa aaatctttcc ggcgggcggt tatataccca
     5701 tgcactcgcg gaactcctcc cgcaatttca tctgaccgcg cctcttgaac gacgcatcac
     5761 tcacgaaagc ctttccctgt taacgccgga tggcgcaacg acgttttcca gcttacagcc
     5821 cggcggtgaa tcctggagtg tattacgtgc acggttcgat ccgtggctgg ttgccgaagc
     5881 cgaagaagaa ggtgtcgaat gcatccccgg agcgacggtg gatgcactgt atgaagaaaa
     5941 cggcagggtg tgtggtgtca tttgtggtga cgatattctc cgtgcccgtt atgtggtgct
     6001 ggcagaaggt gccaacagcg tcctggctga gcgtcacggg ttagtgactc gtcctgctgg
     6061 tgaagcgatg gcgttgggga tcaaagaagt gctgtcgctg gaaccatccg ctattgaaga
     6121 acgttttcat ctggagaata acgaaggcgc agcgttgctg ttcagcggca ggatctgtga
     6181 tgacttaccc ggcggcgcat ttctttatac taatcaacaa acgctctcgt tagggattgt
     6241 ttgcccgctc tcttccctta cgcaaagtcg tgttccggca agcgagctgc tgactcgctt
     6301 taaagcgcat ccggcagtgc gcccgcttat caaaaacacg gaatcactgg agtatggtgc
     6361 gcatctggtg ccagaaggtg gcttgcacag tatgccggtg caatacgccg gtaacggctg
     6421 gctgctggtg ggcgatgcgt tgcgcagttg cgtcaatacc ggaatttccg tgcgcggcat
     6481 ggatatggcg ctgactggcg cgcaggcggc ggcacaaacg ctgataagcg cctgccagca
     6541 ccgcgagccg caaaatctgt ttgcgcttta tcatcacaac gtagagcgca gcctgctatg
     6601 ggatgttcta caacgttatc agcatgttcc ggcgcttttg caacgccctg gctggtatcg
     6661 ggcgtggcct gcgttaatgc aggatatttc ccgcgattta tgggatcagg gtgataaacc
     6721 tgttccaccg ctgcgccagt tattctggcg tcatttacgt cgtcatggcc tgtggcatct
     6781 ggcgggcgat gttatcagga gtctgcgatg tctgtagccc ataatctctg gcgcgttgct
     6841 gatgcgccgc acattgttcc ggctgactcc gttgagcgtc agatggtaca acggttgatt
     6901 aacgcctgtc cggcaggtct tttttcgctc acaccggaag gtgacttacg cgttgattat
     6961 cacggctgcc tggagtgtgg cacctgctgt ttgctgtgcg acgaatccac actacaacag
     7021 tggcgctatc cgccttccgg attcggcatc acctaccgct ttggataaat aataaggata
     7081 atttatgccc ctcttacacc tgctccgcca gaatccggtg attgctgccg ttaaagataa
     7141 tgccagcctg caactggcaa tcgactctga atgccagttt atttccgtgt tgtacggcaa
     7201 tatctgcacc atcagtaata tcgttaaaaa gattaagaac gccgggaaat atgcttttat
     7261 tcatgttgat ttgctggaag gcgcgtcaaa taaagaagtc gtgattcagt ttttgaaact
     7321 ggtgaccgag gcggacggca ttatcagtac caaagcctca atgttgaaag cggcaagagc
     7381 ggaaggtttt ttctgtattc atcgcctgtt tattgttgat tcaatttcgt ttcacaacat
     7441 tgataagcaa gttgcgcaat cgaatccgga ttgtattgag atcctgccag gctgtatgcc
     7501 caaagtgctg ggctgggtga cagagaaaat ccgccaaccg ctgattgccg gtgggctggt
     7561 gtgcgatgaa gaagatgcgc gtaatgcgat taacgcgggt gtcgtggcgc tttccaccac
     7621 gaataccggg gtctggacgt tagcgaaaaa attactttga cgggataatc gtaaccaatt
     7681 gaatttggtt tgatttgtag gccgcacgcc acatccgaca ttcagcgcct gatgcgacgc
     7741 ttgacgcgtc ttatcaggcc tacaagtccc caggccagaa ccgtaggcag gatcatccgg
     7801 caacacgtta ttggcaatct gcgtgaatgt ttgtcaccag tgcctccagc accactttcc
     7861 agtcatcaac cacgccaaca tctgcctgtg aaaataccgc agcgctggcg tcatgattaa
     7921 tcgccaccac aaacttgcta ttgcgtacgc ccgccattaa cgctgctgcg ccagaagcgc
     7981 caacaacaat acacacctca ggtgccagca gatgtccgga aataccaatc actttttcag
     8041 catcaacgcc accgttcatc actcgcgccc gactgtagcc cacttcggct cccagctttt
     8101 ctgccagcat agcgatttcc tggttatctg cctcaccgcc ctgccccacc accagcaccc
     8161 gtctggcctc agctaatggg tcacgggtaa catttttcag atcctctgtg ctaaccagcc
     8221 agtcgggaag cgcgccgggg acaatgttca gttgctgcat accagagggc aacgtggcgt
     8281 ttttcactgc tcccgcctga cgcgccaggg aaaggcatag cgggcgtttt tctgtttgca
     8341 gcgtcgccgt tagcgcattt ccccagtggg atttgcgcac gcttacggtt ggaatatcta
     8401 acgaagtcac ctggcagatg cttgcaccat gtaaacgcca ggccagccgg gtcgacagtt
     8461 catcgccaaa cgttcccggc ggaaagagaa caacgtccgc aggcgttcgc tgccactgtt
     8521 ccaccaacgc atccagcacc tgttccgcca ctacaggctg aggttcaatc tgccagtgcg
     8581 ccagggtaca accgctgaaa tcctgcgccg ccagccagct tgctattgcg gcattctcct
     8641 gatttattgt cacaattgcg atattcatgg ctgcatcctt tggcgtaaat agtcctgcca
     8701 tagcttttga gctttttctg ctaccgtttg cccatcaatc agcgtcgctc cgcgtcgttg
     8761 ctctgctctt gccagttgca gacactgcat agctggcatt tcggcagcaa cagttttacg
     8821 gataatttct gctttccccg cagccattcg ctggcgcatt cccggtactg gcaaggccac
     8881 ttcaccgcac tgacgcacgg caatcaccgc aggcaagcga acccggcaac aacgcaaacc
     8941 atgttcaata cgctgttcga gggtgataaa cagcgcgtcg agagtgaaac gctccacctg
     9001 ggtgaagcag ggccagccca gcatttccgc cagcaaaaat ggcgtttgcc cattctgccc
     9061 ttcgctgctt tggcagccgg taattatcag atccagcgga ttctgatgct gccattcggc
     9121 aatatggcga gcgacaaatt ccggcgcaaa gcgcagatct gccgccgtct ccagcaatac
     9181 agcttcctca aacccaagag ccatgagata gcgcagccag tgcaatgccc gttcatcccc
     9241 catgctcaac gcggttaaag acatcggcgt accgtttttt ctctgcgcca acagcagcgc
     9301 ggcagcggcc tgttcatcag caccgagtaa acttcgcagt agcgaaatat ccggtccgct
     9361 tttaccctga gccgccgcct gccactcttt ttccgccagc attccggcat ccggttcggc
     9421 tttaaacgct aacagaatgt tcattcgcac ctcctacatt tcccgccgcc accagcggga
     9481 gggctttagt ttctggtgcc catagccagg tcacaacaaa accaaccaac aacactgtcg
     9541 ccaggagcaa taaggtgact tgcattcccc actgcgccag cacccacggc agcaggccag
     9601 tacttaccgc cgcgccaagt cgactcatgg cggtggcaaa accgacgccc agcgagcgaa
     9661 tgtcagtagg aaaactttcc gcaggcaaaa tgcccaccag attactgact gccgaaatgg
     9721 tggtgctgaa gagaacaaaa agcagcagcg ttaatgaact gccggaaggc aaacaggcca
     9781 ttactaccag cgttgccgcc agcagcaaaa aacttcccag caaaaatttg cgatgtgcca
     9841 gcaggtgcgt cagaactaat cccagcagcg cgcccacaat taacaacgca ttaagcatca
     9901 ggctggcagt cagcgcatct tccagaccaa tagtctgggc gatagttggc agccaggtat
     9961 aaatcacaaa ccatgggatt acgaggcaga caaagaagac gctgttaaac gccgtgcgcc
    10021 gccagtaacg cgaagagaac aaggttttga tgtgtttatg ggtcgccgtt accacttcat
    10081 cgcccagtaa aacatgggga ccaaaatagc gatgcacgat agcgtgagct tctgcaaaac
    10141 gcccctggcg cagtagccag cgtggtgatt ctggcgttcc ccagcgtaat aacgtaatca
    10201 acaacgcggg cagagctgcc gatgccagta gccagcgcca ggcctccggg ttttcggaaa
    10261 taaagtgatg tccggcaata cttgccagca catagcctac ggtccacacc acgctgaatg
    10321 cgcccagcaa aataccgcga tggcggcgcg gggaaaattc agccagcaag gtgtgaccta
    10381 ctgaataatc gcctcccaga ccaatgccaa tcaaaatgcg cagtccaata agatgctctg
    10441 gcgtggtggc aaaaaattgt aaaaacgaag caagcgtaat cagcaaaaag ctgaaggtga
    10501 agattttttg ccgaccaata tggtcggaga tccacccaag aaccaggctg ccaaggaaca
    10561 aaccaaggag cgccgagccg ccgatcattc ccgccataaa cggcgtcagt tgcatggcgg
    10621 gcgtaagctg aataatggcg taaccaatga cgccgagaac ataaccgtcg gtcaggtgtg
    10681 cgccgaaagt gagcgcagca atgcggcagt gaaaacggtt aagcggtaaa tcatccattc
    10741 gcaccggtga agtgttcatc tttacccctt cattgtccgg taataagaac ggcttctcgc
    10801 ctgagaagcc gaaaaggcaa cgccccgtca tcgcggaacg ctgcctgaca cattattttt
    10861 caatcggata gatagtaccc gtattcataa tgccattagg atcgaactgc tttttcagcc
    10921 cttccagcaa cgcccacgcg ctgccgtgtt ccagtttgct ccagtgaacg cgatgtttac
    10981 cgataccgtg gtggtgcacc atcgaaccgc cgaggcgaat ggtttcttca cagatgatct
    11041 tgttgagcgg attgtggtac ttgtcgattt cctcttccgg cttacagtca acgacgttgt
    11101 aatcgtagac gaagtacatg ttggtgccgt tctgatagct atgagaggaa tgaccgccca
    11161 gcatggtgat gtcgtcggcg tgcgggaact cagtacgaat acggttaata acgctttcgt
    11221 agatttcgtg gatgcagctc cagcagccgg acacttcagt ggtaaagccc atgttgccgg
    11281 ttttgaggat ctgcacacgt tcggcagcca ctttatccgg tccccagttc aggttgttaa
    11341 accaggtttc gatcagtttg ctgtccacgc gctggcactg cgggtagcgg gcaacgattt
    11401 ccgcaatccc ttcgcccgtc gccttcgcaa tgcgagggtt accttcagcc ataaagatca
    11461 gcacacattt tccgtctgca aaatgggtga agtgttgggt gccatcttca gcgtcataca
    11521 aacgagcgat cgacggacga tacccttcca ccatgacttc acgcaggatg ttgaagccgg
    11581 ttttcatgtc ttccaggata tagccgtaga agaggttgtt ttccggggtg aatttaaaga
    11641 ttttcactgt tacttcagtg atatagcaaa gtgcaccttc gttaccgatg atgatgtggc
    11701 gaatgtccgt gccagccgcg cggcgtggca cgtttttaat gcgtgtgacg gtgccatcag
    11761 ccaatactgc ttccagacca acgaccatat cttcgattgc gccgtagagt gtggagaact
    11821 gcccgatact gcgggttgct accaggccgc ccatctgcgc cagcggcttt gactgcggag
    11881 aatgccccgt ggtgtaacct ttttcacgca acgcattttc cagcacttcc tggggaacgc
    11941 cacattgcgc cgtcgcctgc atattctcaa tatcaatatt aatgatttga ttcatggcgg
    12001 aaccgtcgag caccaccgag ttttctacaa cagtttccag cccaccttca gtggcggaag
    12061 cgccggtacg cggcacaccg ttaattttgt gcgcattcat aaaattcagc acacgggaga
    12121 cttgttctgt ggaaccgagt tttacgaccg ctgccggaat cggcaaagta taaataccat
    12181 gaatatccgg aaatttacga aaacggtcaa tactgttctt ctttaatact gtttcatcgg
    12241 taattacgcg atctgcacca acaatttcct ttagttggtc gacaatcgct gcgcgagata
    12301 aagacatagt aattccttcc tgataataaa aatggaataa tcattaataa gagaaaacag
    12361 aaaccaccgt tatggattag cgcactaaat aaccaccatc gaccactaat aaatgcccat
    12421 tgacataatt tgaagccgga cttgcgagga atacagctgc gcccattaaa tcctgagtat
    12481 cgccccaacg gtttgccgga atatgatcaa gaacgcgctg attggtttct ggattactgc
    12541 gtgtcgccag cgtaatatct gttgcataat agccaggggc aataccattt acctgaatat
    12601 tatattgacc tagttcatca caataagctt tggtgaatcc ggcaagagca tgtttagtgg
    12661 cagaatatgc aggtgaccat tgtccaccta agtaagagaa caatgaacag atattaataa
    12721 ttttgccgct tttctgcggg atcataattt ttgcagcttc atagcttaac tcgaatgcgg
    12781 cggtcaggtt cacatcaatc atcggatccc agtcggcacg accgaagtcc agcaccttat
    12841 tcagcttaca aataccggca ttgtttacca gaatatcaac tgtaccgaaa cgctcacagc
    12901 aagcagcgat aatcttctgc ggcgcgcctt ctgcggtgat atccacctgc atgaagtcca
    12961 cctcaacacc ctgtttttca atcatttcct ttgtttcgcc gttatctttg acgaaactag
    13021 gaataaagat atttgcgcca gctttggcca acgccatggc aaatgcctag cctaaaccgc
    13081 tattcccacc ggtaacaatt gcggttttac ctttcagtga gaaaaaatcc attgagaacg
    13141 cattgagaga ttcgattgac atagttagct ccaatttccg taaggcaaaa aaaagaaagg
    13201 taacttcccc cgcgagaggg aagttacctt tctcattatc tctggtaact ggagataatt
    13261 tttagcataa cggcataact gctaatgtga tcgtaatcac agtgtgatat tcgtattgtc
    13321 aaatagtttt aaaaagcaga agcgtgatta ctctcacaca acacctctgc tttccatgtt
    13381 atttcttatt gcaattacta gagacctgag aaaagtaatc cacctgaaag ggcggcttac
    13441 ttttctcttt tttttgtcta ttacaaatgg gaatacgata tgcaacacaa ctcatatcgc
    13501 cgttggataa ccctcgcgat aattagtttt agcggcggcg ttagttttga cctggcttat
    13561 ttacgttata tttatcaaat tcccatggcg aaatttatgg gattcagcaa taccgagata
    13621 ggtttaataa tgagtacctt tggtattgcg gctattattc tttatgcccc cagcggcgtt
    13681 attgccgata aattttcaca ccgcaaaatg attacttccg cgatgatcat taccggatta
    13741 ctgggtctgt taatggcaac gtatccaccg ctgtgggtaa tgctctgtat tcaggtcgcc
    13801 tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga ttaaagccgc ctcgttgctg
    13861 ggcgatcata gtgagcaagg gaaaattatg ggctggatgg aagggctgcg cggcgtcggt
    13921 gtaatgtcgc tggcggtgtt taccatgtgg gtcttttctc gctttgcacc ggatgacagc
    13981 gccagcctga aaacagtcat tatcatctac agtgtggttt acatcttgtt ggggattctg
    14041 tgctggtttt ttgttagcga taacaacaac ctgcgcagtg ccaataacga agaaaaacag
    14101 tcattccagc ttagcgacat ccttgccgtt ttgcgtatca gcaccacctg gtattgcagc
    14161 atggtgattt ttggtgtctt cacaatctac gccattctga gttactccac caactatctg
    14221 accgaaatgt acggcatgtc gctggtggca gcgagctaca tggggattgt gatcaacaaa
    14281 atcttccgcg cgctgtgcgg cccacttggc ggcattatca ccacctacag taaagtgaaa
    14341 tcccctaccc gcgtgatcca aatcctttcc gtactcggcc tgctggcgtt aactgccctg
    14401 ctcgtcacga actctaaccc gcaatcggtc gcgatgggga ttggcctgat tttactgctg
    14461 ggattcacct gttacgcctc acgcgggctg tactgggcct gccctggcga agtgagaaca
    14521 ccgtcttaca ttatgggcac cacggtaggt atttgttcgg tgattggatt cctgccggat
    14581 gtcttcgttt acccgattat cggccactgg caagacactc tgcccgcagc agaagcctac
    14641 cgcaatatgt ggctgatggg aatggcggcg cttgccatgg tgattgtctt tacctttttg
    14701 ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa tggctagcag caagtaagcc
    14761 aggaaaatta caggagaaaa atcatgtcga aaaaatacat catagggatt gatggcggaa
    14821 gtcagagcac aaaagtggtg atgtacgatc tggaaggtag tgtggtttgc gaaggtaaag
    14881 gcttattaca gccgatgcac acgccttatg ccgatactgc agaacatcct gacgacgatt
    14941 tatgggcatc attatgtttt gcaggtcacg atttgatgag ccagtttgcc gggaataaag
    15001 aagatattgt tggcatcggc ctgggctcca tccgttgctg ccgtgcgtta ttgaaagccg
    15061 atggcacgcc tgctgcgccg ttgattagct ggcaggatgc acgcgttaca cgcccttacg
    15121 aacatacgaa tcctgacgtg gcgtatgtca cctctttttc gggttatctg acgcatcgct
    15181 taaccggcga gtttaaagac aatatcgcca actattttgg tcagtggccg gtggattata
    15241 agagctgggc atggagcgaa gatgctgcgg taatggataa gtttaatatc ccccgtcata
    15301 tgctgtttga tgtgcaaatg cctggcaccg tcctcggaca tatcacacca caagccgcac
    15361 tggcgacaca tttcccggca ggactgccgg ttgtttgtac caccagtgat aaaccggtag
    15421 aagctctggg ggccggatta ctggatgatg aaactgcggt aatttctctt ggcacttaca
    15481 tcgcattgat gatgaacggc aaagcactgc cgaaagatcc ggtggcgtac tggccgatta
    15541 tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg tattcgcaaa ggtatgtgga
    15601 cggtgagctg gctgcgtgac atgttaggcg agtcgttaat tcaggatgcc agggcgcagg
    15661 atctttcacc ggaagattta ctcaacaaga aagcctctag tgtgccacct ggctgtaatg
    15721 ggctgatgac ggtgctggac tggctgacca atccgtggga accgtacaaa cgcgggatta
    15781 tgatcggctt tgattccagc atggattacg catggatata tcgttcgata ttggaaagcg
    15841 tggcgctaac gctgaagaac aattacgaca atatgtgtaa tgaaatgaat cactttgcga
    15901 agcatgtgat cattactggc ggcggttcga acagcgatct gtttatgcaa atttttgccg
    15961 acgtgttcaa ccttccggca cgccgtaacg ccattaacgg ttgtgcaagt ctgggagcag
    16021 cgattaatac agcggtaggt ctggggctat acccggatta cgcaacggct gttgataaga
    16081 tggttcgcgt gaaagatatc tttataccga ttgagagcaa tgccaaacgc tacgacgcga
    16141 tgaataaagg cattttcaaa gacctaacca aacatactga tgtgatcctg aaaaaatcgt
    16201 atgaagtgat gcatggggaa ttggggaatg tggattcgat ccagagctgg tcgaatgcgt
    16261 gaatgaggta ggccgggcgg aaaactgccc ggcctgaaca tacctaaatg gttatccccg
    16321 ctgacgcggg gaacataagt tcgacaactt aaaaaatgat aacaagtccg gtttatcccc
    16381 gctgatgcag ggaacataat aaaaacttta actagttcgg tttatccctg tttttacaag
    16441 gaatattaac tacactcgat gtctttagaa ttaaagacaa tacgaatcgc actttatcaa
    16501 ttttcactta aagtatgaac tggatatctt tattcataag aactattca
//
LOCUS       DAZFJF010000104        15604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFJF010000104 DAZFJF010000000
VERSION     DAZFJF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.104"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            100..342
                     /gene="yjbE"
                     /locus_tag="ACHKAU_003672"
     CDS             100..342
                     /gene="yjbE"
                     /locus_tag="ACHKAU_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO5532658.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            456..1094
                     /locus_tag="ACHKAU_003673"
     CDS             456..1094
                     /locus_tag="ACHKAU_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO5532659.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1091..1828
                     /locus_tag="ACHKAU_003674"
     CDS             1091..1828
                     /locus_tag="ACHKAU_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5532660.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1828..3924
                     /locus_tag="ACHKAU_003675"
     CDS             1828..3924
                     /locus_tag="ACHKAU_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5532661.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3971..4249)
                     /locus_tag="ACHKAU_003676"
     CDS             complement(3971..4249)
                     /locus_tag="ACHKAU_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532662.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4250..4393
                     /locus_tag="ACHKAU_003677"
     CDS             4250..4393
                     /locus_tag="ACHKAU_003677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532663.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4519..4929
                     /gene="psiE"
                     /locus_tag="ACHKAU_003678"
     CDS             4519..4929
                     /gene="psiE"
                     /locus_tag="ACHKAU_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO5532664.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4973..6448)
                     /gene="xylE"
                     /locus_tag="ACHKAU_003679"
     CDS             complement(4973..6448)
                     /gene="xylE"
                     /locus_tag="ACHKAU_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO5532665.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6820..7710)
                     /gene="malG"
                     /locus_tag="ACHKAU_003680"
     CDS             complement(6820..7710)
                     /gene="malG"
                     /locus_tag="ACHKAU_003680"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO5532666.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7725..9269)
                     /gene="malF"
                     /locus_tag="ACHKAU_003681"
     CDS             complement(7725..9269)
                     /gene="malF"
                     /locus_tag="ACHKAU_003681"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO5532667.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9423..10613)
                     /gene="malE"
                     /locus_tag="ACHKAU_003682"
     CDS             complement(9423..10613)
                     /gene="malE"
                     /locus_tag="ACHKAU_003682"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO5532668.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10978..12093
                     /gene="malK"
                     /locus_tag="ACHKAU_003683"
     CDS             10978..12093
                     /gene="malK"
                     /locus_tag="ACHKAU_003683"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO5532669.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12165..13505
                     /gene="lamB"
                     /locus_tag="ACHKAU_003684"
     CDS             12165..13505
                     /gene="lamB"
                     /locus_tag="ACHKAU_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO5532670.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13748..14668
                     /gene="malM"
                     /locus_tag="ACHKAU_003685"
     CDS             13748..14668
                     /gene="malM"
                     /locus_tag="ACHKAU_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO5532671.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14897..15604
                     /locus_tag="ACHKAU_003686"
                     /pseudo
     CDS             14897..15604
                     /locus_tag="ACHKAU_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579094.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3922 a         3947 c         4056 g         3679 t
ORIGIN      
        1 ttccccgcgc cgtaattcgc atgctttagt tgtgtatact cgatcccgcc cgaaatgttt
       61 ttgggtaaat ctccattcat tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc
      121 atttttgcca tatctgcgct tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc
      181 gaagcggcag ggtcggcagc aacgtcggtt tcggcgggga gttcctccgc taccagcgtc
      241 agcaccgtaa gctcggcggt gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct
      301 aataccggga ccaccacaac cacgaccacc agtacccagt aataaagtat gtatccccaa
      361 aataatttga gtcattgcat ctgtggcttg aagtatgaag ggatttacca taaccacact
      421 ccggtgtggt tattctgccc ctctggagaa gagtcgtgaa gcgacctgca ctcattctta
      481 tctgcctgct attacaggcc tgttcagcca cgactaaaga gctgggcaat tcactgtggg
      541 acagtctgtt cggcacgcca ggcgtacagc tgacggacga tgatattcaa aatatgccct
      601 acgccagcca gtacatgcag cttaatggcg ggccgcaatt gtttgtggtg ctggccttcg
      661 ctgaagacgg acaacaaaaa tgggtcactc aggatcaagc cactcttgtc acacaacatg
      721 gtcgtctggt gaagactttg cttggcggcg acaacctgat tgaagtgaat aaccttgccg
      781 ccgacccgct gattaaaccc gcacaaattg ttgatggtgc aacatggacg cgcacgatgg
      841 gctggaccga gtaccagcag gtacgctacg ccaccgcacg ctcagtcttc aaatgggatg
      901 gcaccgatac cgtcaaagtc ggcagcgatg aaaccctggt tcgcgtgctg gacgaagaag
      961 tctccaccga ccaggcgcgc tggcataacc gctactggat cgacagcgaa gggcaaattc
     1021 gccagtcgga acagtatctc ggcgcggatt atttcccggt gaaaaccact ctcatcaagg
     1081 cggcaaaaca atgattaaac aaacttttgt cgcgttgctt ttgagtgtgg gagcgtcatc
     1141 ggtcttcgcg gcaggaaccg tcaaggtgtt cagcaatggc agcagtgagg ccaaaacact
     1201 aacaggcgca gagcatttaa tcgatctggt aggccaaccg cggctggcaa acagctggtg
     1261 gcccggtgcg gtgattagcg aagagctggc aacggcggca gcattgcgtc agcagcaggc
     1321 gttgctgaca cggctggcag aacagggcgc agattccagc accgacgatg ccgccgccat
     1381 taacgcctta cgccagcaaa tccaggcatt gaaagtgaca ggcaggcaaa aaatcaatct
     1441 tgatcccgat atcgtccgcg ttgccgaacg cggtaacccg ccgttgcagg gcaactacac
     1501 gctgtgggtc ggaccaccgc cgtccacggt cacgttgttc gggcttatca gccatcctgg
     1561 caatcagcca ttcactccag gtcgcgacgt ggcgagctat ctctccgggc agaacctgct
     1621 tagcggtgcg gatcgcagct acgcgtgggt cgtttacccg gacggacgca cgcaaaaagc
     1681 gccggtggct tactggaaca agcgtcacgt agagccgatg cccggcagca ttatttatgt
     1741 tggcctcgcg gactccgtct ggagtgagac gcctgatgcc cttaacgccg acattcttca
     1801 gactctgacg cagcggatac cccaataatg aaaaaaagac atctgcttag cttactggcg
     1861 ctgggcatta gcatggcttg ctacggcgaa acatatcctg cgcccattgg cccgtcgcag
     1921 tcggatttcg gtggcgtagg attattacaa actcccaccg cgcgtatggc acgggaaggg
     1981 gagttgagtc tgaactatcg cgataacgat cagtaccgtt attactcagc ttcagtgcaa
     2041 ctcttcccgt ggctggaaac aacgctgcgc tacaccgacg tgcgcacccg gcagtacagc
     2101 agcgtcgaag cgttctctgg cgatcaaacg tataaagata aagccttcga tctcaaactg
     2161 cgtttgtggg aagagagtta ttggctgccg caagtggcgg ttggtgcgcg ggatatcggc
     2221 ggtacggggc tgtttgatgc ggaatatctt gttgccagca aagcctgggg gccgttcgat
     2281 tttacgctcg gcctgggctg gggatatctg ggcaccagcg gtaatgtgaa aaatccgctc
     2341 tgttcagcca gtgataaata ttgctatcgc gataacagct acaaacaggc gggatctatc
     2401 gacggtagcc agatgttcca cggtcctgcc tcactgtttg gcggcgtgga ataccagacg
     2461 ccctggcaac cgctgcgcct gaaactggag tatgaaggca ataattatca gcaggatttt
     2521 gccgggaagc tggagcaaaa aagtaagttt aacgtcggtg cgatttatcg cgttaccgat
     2581 tgggccgacg ttaaccttag ctatgaacgt ggcaacacct ttatgtttgg cgttacgttg
     2641 cgcaccaact ttaacgatct gcgcccgtct tacaacgata acgcccgccc gcaatatcaa
     2701 ccgcagccgc aggatgccat tttgcagcat tcggtggtgg cgaatcagtt aacgttgctg
     2761 aaatacaatg ccggacttgc cgatccgcag atccaggcga aaggcgatac gctgtatgtt
     2821 accggcgagc aggtgaaata tcgtgattcg cgcgaaggga tcatccgtgc gaatcggatc
     2881 gtgatgaacg atctgccgga tgggatcaaa acgatccgca ttacagaaaa tcgccttaac
     2941 atgccgcagg tgacgacgga aaccgatgtc gccagcttga aaaatcatct cgccggggag
     3001 ccgctgggcc atgaaacgaa ggtggcgcaa aaacgcgtcg agccagtggt tccgaagtcc
     3061 accgagcagg gatggtatat cgacaaatca cgctttgatt tccatatcga tccggtgctg
     3121 aaccagtcgg tcggtggccc ggaaaacttt tacatgtatc agctgggcgt gatgggaacg
     3181 gcagatttgt ggctgacgga ccatctgctg accaccggca gcctgtttgc aaatcttgcc
     3241 aacaactacg acaagtttaa ctacaccaat ccgccgcagg actcgcactt accgcgcgtt
     3301 cgtacccatg tgcgcgagta tgtgcagaac gatgtctatg tgaataacct gcaagccaac
     3361 tacttccagc atctgggcaa cggcttctac ggtcaggtct atggaggtta tctcgaaacc
     3421 atgtttggcg gtgccggggc agaagtgttg tatcgtccgc tggacagcaa ctgggcgttt
     3481 ggtctggatg ccaactacgt taaacagcgc gactggcgta gtgcaaaaga tatgatgaaa
     3541 ttcaccgact acagcgtgaa aaccggacat ctgaccgcct actggacgcc atctttcgcc
     3601 caggacgtgt tggtgaaagc gagcgtcggg cagtatctgg caggggataa aggcggcacg
     3661 ctggagatcg ccaaacgctt tgatagcggc gtggtggttg gcggctatgc cacgatcacg
     3721 aatgtttcga aagaggagta cggcgaaggg gacttcacca aaggcgtgta tgtctctgtg
     3781 ccgttggatc tcttctcgtc tggcccgaca cgcagccgtg cggcgattgg ctggacgccg
     3841 ctgacgcgtg acggtggtca gcaacttggg cgtaagttcc agttgtatga catgaccagc
     3901 gaccgtagcg tcaatttccg ctaagtcatg ggaaaggtgc cagttttcgc aatcactggc
     3961 accctcattc ttaatggcct gttgagccat caggaacgca gttcaccccc ggtttgcgat
     4021 ctggtgtgta aaggcgaaaa cattctgtct tcctgtccgt cgatatcggt tgcagatctg
     4081 gttgcctgag cgactgggcc atcattgggc tggagacatt gctttgcggt aggtcggctt
     4141 tatcagcagt accgagcgga ccagcataag caggaagaac agagactgat aacatcaaag
     4201 cagcaaaata aggcttcatt tttaccacct ttatcaggtt acgtttcatt tgttccagag
     4261 gaacattgtc gatttttcgc gcattgctgg tggctgggaa tcacctgaat gggtgatttt
     4321 tgaattaccg gctttggtgc ggtttgtctt gccggatgcg ccgccaggcg cggcttatcc
     4381 ggcctacggg taggtatatc cggctttggt ggaggcgcgc tccaaatcca ggttgaacaa
     4441 aacatacaca aaaaatatag atctccgtca catttttgcg ttatacagga agctcgccac
     4501 tgtgaaggag gtactgctat gacgtcactc tctcgtccgc gcgtggagtt tatctccacc
     4561 attttgcaga ccgtactcaa tcttggcctg ttgtgcctcg gcctgatttt ggttgtcttc
     4621 ctcggcaaag aaacggtgca tctggctgat gtgctgttcg cgccagaaca aaccagcaaa
     4681 tatgagctgg tagaagggct ggtggtttac tttctctatt tcgaatttat cgcgctgatt
     4741 gtgaagtact ttcagtccgg ttttcacttc ccgctacgtt actttgtcta tattgggatc
     4801 accgccattg tgcgcttgat catcgtcgat cataaatcgc cactggatgt attgatctac
     4861 tcggcagcga tcctgttact ggtgatcacc ctgtggctgt gtaattcgaa acggctgaag
     4921 cgggagtaaa aagacagcac gccgaactgg cgcggcgtgc tggacaggaa gattacagtg
     4981 tagcagtttg ttgtgttttc ttcgtttccg gttcccagag ggcttccagc tcctcaaggg
     5041 ttttaccttt ggtttccggg acaaatttcc acataaatag tgctgccaga acgcccatac
     5101 aaccgtaaat ccagtaggag aaaccgttgt ggaaatgggt caccagccag gagtttttgt
     5161 ccatcatcgg gaaggtccag gagacgaagt agttcgccag ccactgggcc gccaccgcga
     5221 ttgccagcgc tttaccacga atagcattcg ggaagatttc cgacagcagt acccagcata
     5281 ccggacccca ggacatggca aaggcagcaa catagaacag catcgacagt agcgccacaa
     5341 tacccggtgc ctgagtgtaa aacgcggtac cgaggctaaa cataccgatt gccattccga
     5401 gcgcgccgat aatttgcagt ggcttacgac caaatttatc caccgtcata attgccagaa
     5461 cggtgaaggt gaggttgata actccgacaa taatggtctg caacagcgcg atatccgtgc
     5521 tggcccccag cgttttgaac acttccggcg cgtagtacaa caccacattg atgccgacaa
     5581 attgctggaa gatggagagc ataacgccga taacaatcac gcccacgccg aacatcagca
     5641 gacgaccacc ggttttgcgt ccatgatcca gggagtgttt aatttcctgc accgcctggg
     5701 ctgccaaagc gttgcccata attttgcgca ggatgccttc cgcttgttct tgcttaccgc
     5761 gtgacatcag ccagcgaggg ctttccggca cggtataaag cagcattaag aacagcagcg
     5821 cagggataca ttccgaagca aacatataac gccagccgtc agtattcagc caggtggcat
     5881 cgccggaacg ggcaataaaa tagtttacgc agtaaactaa aagttggccg aaaataatcg
     5941 caaactggtt aaaagagacc agcttcccac gaatatgtgc aggagccagt tccgcaatat
     6001 acattggtga gagcattgag gctaaaccaa caccaatacc gccaataatg cgataaataa
     6061 caaattccgg gacataacct gccagataaa caggcactgt gttgtccggg tttatagagg
     6121 taaaaccaag ttctggccag gcagaaccta caccagaaat aaaaaacagg acagcagcaa
     6181 tcttaagtga atcacgacga ccgaagcggt tactgcaata accaccgagg gcaccgccga
     6241 tgatgcaacc aatcagagcg ctggctacgc aaaaccctaa cagggagttg gcagcggatt
     6301 cacttaagtt ttgtggagca acaaagacgg tatggagtga ctcaacagta ccggaaataa
     6361 cggcggtgtc gtagccaaat aataaaccac ctaatgtagc gactaaggta atcgaaaata
     6421 tataactgga attatactgg gtattcattc agacctgcct tagaccattc taatctttta
     6481 tcagtgcatt gatgttggaa ttgtcaggca gttaaggcaa ttatcatttt ttacaatgcg
     6541 tttacgtaat gtcttttctg tgatcttaat tgtgataatt atctaaaatt gatgaaaaca
     6601 atactattgc cgtgactcag agcacgaaag agaattatcg taagtgggaa aacaaataac
     6661 gtaaaaataa taagctctat gatgggaata ttaaccggcg aacgattcag attgcagacg
     6721 aaagaaaaaa aggcgctccg tggagcgccg aataacagtc acaagttggg ataacgtaag
     6781 ttgagggtgc agcggcataa cattggcaga acaacatctt taacctttca caccacctgc
     6841 cgtcaggccg ttgaccagcc agcgttgagc cagcaagaag acgatggtga tcggtaatgc
     6901 agacatcacg gcagcggcgg caaagtcacc ccacaggtag ttttgcgggt tgaggtattg
     6961 ctgcatcccc acggccaggg tgtagctgtt tacgtcacgc agtaacagcg acgcgaccgg
     7021 aacttcagta atggcagcga taaacgacaa gatgaatacc accgccagaa tcggtactga
     7081 cagcggcaac aggacaaggc ggaaggcctg ccacggtgtc gcaccatcca gcgcagccgc
     7141 ttcttccagc gaactatcga tggtttcgaa atagcctttg atggtccaga catgcagcgc
     7201 aatcccaccc agatacgcga aaattacgcc gccgtgagta ttcaggccaa tgaatggaat
     7261 gtactcaccc agacgatcaa acaacgcata caacgcgacc agtgaaagta ctgccgggaa
     7321 catctggaaa atcagcattc ctttcagcag cgtcgctttg cctggaaagc gcatacgggc
     7381 gaaagcgtag gcgcaggtgg tagagagcgc cacaatgcca atcgcggaaa tcccggcgac
     7441 ctttaccgag ttccacagcc acagcagtac cgggaatggc ggtggcgtaa tgcgaccatc
     7501 agcctgttca acgctaaaac ctaacgccag tttccagtga tcccaggaga tttgctccgg
     7561 gatcaggcta ccggtcgcga agtttccctg acgcagcgag atagcgacga ccatcagcag
     7621 cgggaacata atcgccgcga taaaaagtag cagtagcagg tgagtaataa acaaacgtgc
     7681 tttttgcgat ttcggttgga ccattgccat ttttgttatc tcccttaatc aaacttcatt
     7741 cgcgtggctt tcaggttcac tatcgccagc gcacccacca gcaggaagat cagcgtggca
     7801 attgctgctg ccagaccgaa gtcctgaccc ccgccgcctt caaaagcgat gcggtaggtg
     7861 tagttaacaa gcaggtcggt ataaccggct ggcgtggtcg tgccaagacg atccgggccg
     7921 ccgttggtta acagttgaat cagcacgaag ttgttaaagt taaaggcgaa gctggcgatc
     7981 atcagcggcg tcagcggttt aatcagcagc ggcagcgtaa tcttaaagaa gttctggaac
     8041 ggacctgcgc catccattgc tgaggcttca tacaaatcgt ccggaatcgc tttcagcaag
     8101 cccatgcaga ggatcatcat gtacggataa cccagccagg tattgacgat aatcagcatc
     8161 gtgcgggcgg tggtcggatc gctgaaccag gcgggcttca cgccaaacag cgcgctcaac
     8221 atcatgttga tttcaccgaa gctctggtta aacaaccctt tgaaaatcaa gattgaaatg
     8281 aacgatggca ccgcgtaggg cagaatcagc aggacgcgat agaccgcttt gccgcgcaac
     8341 gcttcccact gcaccagaca cgccagaacc atgccgaccg ccaccgttaa aaagacagtg
     8401 atcagcgaga acaccacggt ccagacgaaa atggcgagga acggtttctg aatgccttcg
     8461 tcggtgaaga cgcgggtaaa gtttttccag ccagtagtca cggtgtaacc ggggcttagc
     8521 ttttcatcgc cccagttgcc gtcggcggta atggactggt aaaagccaat ttggttattc
     8581 ggacgatatt tcacgccgct ctgattattc gtcaacgtgc cgtcaccgtc gagtgtgtag
     8641 agcggctgcg tgccagaaaa ctggcgcagg gagctcatca tcactttgtt gccatccggc
     8701 agaatggcgg taatgtcact cagcgcctga cgattctggg taatcacgcg cagattcgcg
     8761 cgttcgcctt cgggctgggt ggtcgtttct ttcagttgca gtttttgctc gccgccaaat
     8821 ttaaaagcgt cggagaggta atttttgccg gtttcgccgt cgctgagggc tagttgccac
     8881 tcatcgcccg ccgggtaaag accaaaatta taggttttgc ctgcttgcca ggagcgatct
     8941 agcaacactt cctgcgcacg ttcaaaagtc agctggttag tgctgctgta gttggtgaag
     9001 gcaatggcga tggtgcagac cagagggaag aggacgaata atcccattcc agccattccc
     9061 gggtaaacat agcgccaggc gtaggcttta cgattggcga aaatatacag ccccgctgaa
     9121 ctcaatatca gcgtggtaat ggcgaacagg tattcccctt gtgcgtacat taaaacaaca
     9181 aggtaaccca ccagcaggcc gagcagacct aacactgacc atttcagcgc gtcgctttgc
     9241 caccaatgtt tctttttaat gacatccatg gggttcttcc tcattccagg acggataagg
     9301 ctttcacgcc ttatccgaca acaactgcct gatgcgacgc tgacgcgtct tatcaggcct
     9361 acatacgttt cggttttgta ggccggacaa ggcgttcacg ccgcatccgg catttcacag
     9421 cattacttgg tgatacgagt ctgcgcgtct ttcagggctt catcgacagt ctgacgaccg
     9481 ctggcggcgt tgatcaccgc agtacgcacg gcataccaga aagcggacat ctgcgggatg
     9541 ttcggcatga tttcaccttt ctgggcgttt tccatggtgg ctgcaatacg tggatctttc
     9601 gccaactctt cctcgtaaga cttcagcgct acggcaccca gcggtttgtc tttattaacc
     9661 gcttccagac cttcgtcagt cagcagatag ttttcgagga actctttcgc cagctctttg
     9721 ttcggactgg cggcgttaat accggcgctc agcacgccaa cgaacggttt agatggttga
     9781 cccttgaagg tcggcagtac cgttacacca taattcactt tgctggtgtc gatgttggac
     9841 catgcccacg ggccgttgat ggtcatcgct gtttcgcctt tattaaaggc agcttctgcg
     9901 atggagtaat cggtgtctgc attcatgtgt ttgtttttaa tcaggtcaac caggaaggtc
     9961 agacccgctt tcgcgccagc gttatccacg cccacgtctt taatgtcgta tttgccattt
    10021 tcatacttga acgcataacc cccgtcagca gcaatcagcg gccaggtgaa gtacggttct
    10081 tgcaggttga acatcagcgc gctcttacct ttcgctttca gttctttatc cagcgccggg
    10141 atctcttccc aggtttttgg tgggttcggc agcagatctt tgttataaat cagcgataac
    10201 gcttcaacag cgatcgggta agcaatcagc ttgccgttgt aacgtacggc atcccaggta
    10261 aacggataca gcttgtcctg gaacgctttg tccggggtga tttcagccaa caggccagat
    10321 tgagcgtagc caccaaaacg gtcgtgcgcc cagaagataa tgtcagggcc atcgccagtt
    10381 gccgcaacct gagggaattt ctcttccagt ttatccggat gctcaacggt gactttaatt
    10441 ccggtatctt tctcgaattt cttaccgact tcagcgagac cgttatagcc tttatcgccg
    10501 ttaatccaga ttaccagttt accttcttcg attttggcga gagccgaggc ggaaaacatc
    10561 atcgtcgtta atgcggataa tgcgaggatg cgtgcacctg tttttatttt cataatctat
    10621 ggtccttgtt ggtgaagtgc tcgtgaaaac acctaaacgg actctagttt ctttatacgg
    10681 caacctcttt ccatcctcct tgcccctacg ccccaccgtt gctttgtgtg atctctgtta
    10741 cagaattggc ggtaatgtgg agatgcgcac ataaaatcgc cacgattttt gcaagcaaca
    10801 tcacgaaatt ccttacatga cctcggttta gttcacagaa gccgtgttct catcctcccg
    10861 cctcctcccc cataaaaaag ccagggggtg gaggatttaa gccatctcct gatgacgcat
    10921 agtcagccca tcatgaatgt tgctgtcgat gacaggttgt tacaaaggga gaagggcatg
    10981 gcgagcgtac agctgcaaaa tgtaacgaaa gcctggggcg aggtcgtggt atcgaaagat
    11041 atcaatctcg atatccatga aggtgaattc gtggtgtttg tcggaccgtc tggctgcggt
    11101 aaatcgactt tactgcgcat gattgccggg cttgagacga tcaccagcgg cgacctgttc
    11161 atcggtgaga aacggatgaa tgacactccg ccagcagaac gcggcgttgg tatggtgttt
    11221 cagtcttacg cgctctatcc ccacctgtca gtagcagaaa acatgtcatt tggcctgaaa
    11281 ctggctggcg caaaaaaaga ggtgattaac caacgcgtta accaggtggc ggaagtgctg
    11341 caactggcgc atttgctgga tcgcaaaccg aaagcgctct ctggcggtca gcgtcagcgt
    11401 gtggcgattg gccgtacgct ggtggccgag ccaagcgtat ttttgctcga tgaaccgctc
    11461 tccaacctcg atgctgcact gcgtgtgcaa atgcgtatcg aaatctcccg tctgcataaa
    11521 cgcctgggcc gcacaatgat ttacgtcacc cacgatcagg tcgaagcgat gacgctggcc
    11581 gacaaaatcg tggtgctgga cgcaggtcgc gtggcgcagg ttgggaaacc gctggagctg
    11641 taccactatc cggcagaccg ttttgtcgcc ggatttatcg gttcgccaaa gatgaacttc
    11701 ctgccggtaa aagtgaccgc caccgcaatc gatcaagtgc aggtggagct gccgatgcca
    11761 aatcgtcagc aagtctggct gccagttgaa agccgtgatg tccaggttgg agccaatatg
    11821 tcgctgggta ttcgcccgga acatctactg ccgagtgata tcgctgacgt catccttgag
    11881 ggtgaagttc aggtcgtcga gcaactcggc aacgaaaccc aaatccatat ccagatccct
    11941 tccattcgtc aaaacctggt gtaccgccag aacgacgtgg tgttggtaga agaaggtgcc
    12001 acattcgcta tcggcctgcc gccagagcgt tgccatctgt tccgtgagga tggcactgca
    12061 tgtcgtcgac tgcataagga gccgggcgtt taagcacccc acaaaacaca caaagcctgt
    12121 cacaggtgat gtgaaaaaag aaaagcaatg actcaggaga tagaatgatg attactctgc
    12181 gcaaacttcc tctggcggtt gccgtcgcag cgggcgtaat gtctgctcag gcaatggctg
    12241 ttgatttcca cggctatgca cgttccggta ttggctggac aggtagcggc ggtgaacaac
    12301 agtgtttcca gactaccggt gctcaaagta aataccgtct tggcaacgaa tgtgaaactt
    12361 atgctgaatt aaaattgggt caggaagtgt ggaaagaggg cgataagagc ttctatttcg
    12421 acactaacgt ggcctattcc gtcgcgcaac agaatgactg ggaagctacc gatccggcct
    12481 tccgtgaagc aaacgtgcag ggtaaaaacc tgatcgaatg gctgccaggt tccaccatct
    12541 gggcaggtaa gcgcttctac caacgtcatg acgttcatat gatcgacttc tactactggg
    12601 atatttctgg tcctggtgcc ggtctggaaa acatcgatgt tggcttcggt aaactctctc
    12661 tggcagcaac ccgctcctct gaagctggtg gttcttcctc ttttgccagc aacaatattt
    12721 atgactatac caacgaaacc gcgaacgacg ttttcgatgt gcgtttagcg cagatggaaa
    12781 tcaacccggg cggcacatta gaactgggtg tcgactacgg tcgtgccaac ctgcgtgata
    12841 actatcgtct ggttgatggc gcatcgaaag acggctggtt attcactgct gaacatactc
    12901 agagtgtcct gaagggcttt aacaagtttg ttgttcagta cgctactgac tcgatgacct
    12961 cgcagggtaa aggtctgtcg cagggttctg gcgtcgcgtt tgataacgaa aaatttgcct
    13021 acaatatcaa caacaacggt cacatgctgc gtatcctcga ccacggtgcg atctccatgg
    13081 gcgacaactg ggacatgatg tacgtgggta tgtaccagga tatcaactgg gataacgaca
    13141 acggcaccaa gtggtggacc gttggtattc gcccgatgta caagtggacg ccaatcatga
    13201 gcaccgtgat ggaaatcggc tacgacaacg tcgaatccca gcgcaccggc gacaagaaca
    13261 atcagtacaa aattaccctc gcacaacaat ggcaggctgg cgacagcatc tggtcacgcc
    13321 cggctattcg tgtcttcgca acctacgcca agtgggatga gaaatggggt tatgactaca
    13381 acggcgatag caaggttaac ccgaactacg gcaaagccgt tcctgctgat ttcaacggcg
    13441 gcagcttcgg tcgtggcgac agcgacgagt ggaccttcgg tgcccagatg gaaatctggt
    13501 ggtaatagca aaacctgggc cggataaggc gtttacgccg cattcggcaa ccaacgcctg
    13561 atgcgacgct tgcgcgtctt atcaggccga caacggctgt caaatgtagg ccggataagg
    13621 cgtttacgcc gcatccggca taaaaacagg ttgtcattat ctgaaagggg cgaaagcccc
    13681 tctgattatc gggtttagcg cgctattgcc tggctaccgc tgagctccag attttgaggt
    13741 gaaaacaatg aaaatgaata aaagtctcat cgccctctgt ttatcagcag ggttactggc
    13801 aagcgcgcct ggaatcagcc ttgccgatgt taactacgta ccgcaaaaca ccagcgacgc
    13861 gccagccatt ccatctgctg cgctgcaaca actcacctgg acaccggtcg atcaatctaa
    13921 aacccagacc acccaactgg cgaccggcgg ccaacaactg aacgttcccg gcatcagtgg
    13981 tccggttgct gcgtacagcg tcccggcaaa cattggcgaa ctgaccctga cgctgaccag
    14041 cgaagtgaac aaacaaacca gcgtttttgc gccgaacgtg ctgattcttg atcagaacat
    14101 gaccccatca gccttcttcc ccagcagtta tttcacctac caggaaccag gcgtgatgag
    14161 tgcagatcgg ctggaaggcg ttatgcgcct gacaccggcg ttggggcagc aaaaacttta
    14221 tgttctggtc tttaccacgg aaaaagatct ccagcagacg acccaactgc tcgacccggc
    14281 taaagcctat gccaagggcg tcggtaactc gatcccggat atccccgatc cggttgctcg
    14341 tcataccacc gatggcttac tgaaactgaa agtgaaaacg aactccagct ccagcgtgtt
    14401 ggtaggacct ttatttggtt cttccgctcc agctccggtt acggtaggta acacggcggc
    14461 accagctgtg gctgcacccg ctccggcacc ggtgaagaaa agcgagccga tgctcaacga
    14521 cacggaaagt tattttaata ccgcgatcaa aaacgctgtc gcgaaaggtg atgttgataa
    14581 ggcgttaaaa ctgcttgatg aagctgaacg cctgggatcg acatctgccc gttccacctt
    14641 tatcagcagt gtaaaaggca aggggtaatt acgccccaca gtgctgattt tgcaacaact
    14701 ggtgcgtctc ctggcgcacc tttttttatg cttccttcct gggatatgag cgatttttta
    14761 tagtaactca cttcttcttc actaagaata tccattatct caatgcctta tcagagattc
    14821 ttttcctttc gccggtagtg tctggacatt caggctactt ttccaggtta ttttatttct
    14881 gttatgcaga ggttttatga taagtcatat cctaaattct ggcggcaata actctttgat
    14941 gaaacatgat gtggtgcaag gaaataatat agtagatctt gatttactac gtaatttcaa
    15001 tggggtgcca ggtttaaaca gagataactt tatttatatc agcaatattt tttcaaatat
    15061 aaaacaacgg aacgaaaaaa tcatgcaata aatttgtttc gtgaagtctc aatcagtaat
    15121 gatactataa gtgtaaaatt ctacagaaat gaaaaaaatt gaatgcgctt gcaattttct
    15181 gatggataaa gatgcgcagg ggtatatcga cctgtctgat ttggatttaa caagttgtca
    15241 ttttaaaggt gacgttatat cgaaggtgtc ttttttatca tcaaatctac aacatgtaac
    15301 attcgaatgt aaagaaattg gggattgcaa ttttactact gcaatagttg ataatgtcat
    15361 atttagatgt cgacgtttac acaatgtgat ttttatcaaa gcgagtggtg aatgtgtcga
    15421 tttcagcaaa aatattcttg atacagttga cttctcgcag agtcaactta ctcagagtaa
    15481 ttttcgcgaa tgtcagatta gaaattcaaa cttcgataat tgttatcttt acgcttcgca
    15541 cttcaccaga gcagagtttc tgtctgccaa agaagtatca tttattaaat cgaatatgac
    15601 agct
//
LOCUS       DAZFJF010000105        15107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFJF010000105 DAZFJF010000000
VERSION     DAZFJF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.105"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            162..1220
                     /gene="citC"
                     /locus_tag="ACHKAU_003687"
     CDS             162..1220
                     /gene="citC"
                     /locus_tag="ACHKAU_003687"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO5532672.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1235..1531
                     /gene="citD"
                     /locus_tag="ACHKAU_003688"
     CDS             1235..1531
                     /gene="citD"
                     /locus_tag="ACHKAU_003688"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO5532673.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1528..2436
                     /gene="citE"
                     /locus_tag="ACHKAU_003689"
     CDS             1528..2436
                     /gene="citE"
                     /locus_tag="ACHKAU_003689"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO5532674.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2447..3979
                     /gene="citF"
                     /locus_tag="ACHKAU_003690"
     CDS             2447..3979
                     /gene="citF"
                     /locus_tag="ACHKAU_003690"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO5532675.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3983..4534
                     /gene="citX"
                     /locus_tag="ACHKAU_003691"
     CDS             3983..4534
                     /gene="citX"
                     /locus_tag="ACHKAU_003691"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO5532676.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4509..5387
                     /gene="citG"
                     /locus_tag="ACHKAU_003692"
     CDS             4509..5387
                     /gene="citG"
                     /locus_tag="ACHKAU_003692"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO5532677.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5438..6901
                     /gene="citT"
                     /locus_tag="ACHKAU_003693"
     CDS             5438..6901
                     /gene="citT"
                     /locus_tag="ACHKAU_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO5532678.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7015..7821
                     /gene="rna"
                     /locus_tag="ACHKAU_003694"
     CDS             7015..7821
                     /gene="rna"
                     /locus_tag="ACHKAU_003694"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO5532679.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8051..8461
                     /gene="rnk"
                     /locus_tag="ACHKAU_003695"
     CDS             8051..8461
                     /gene="rnk"
                     /locus_tag="ACHKAU_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO5532680.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8599..8688
                     /gene="yldA"
                     /locus_tag="ACHKAU_003696"
     CDS             8599..8688
                     /gene="yldA"
                     /locus_tag="ACHKAU_003696"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO5532681.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8692..9930)
                     /gene="ybdR"
                     /locus_tag="ACHKAU_003697"
     CDS             complement(8692..9930)
                     /gene="ybdR"
                     /locus_tag="ACHKAU_003697"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO5532682.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10151..10579
                     /gene="uspG"
                     /locus_tag="ACHKAU_003698"
     CDS             10151..10579
                     /gene="uspG"
                     /locus_tag="ACHKAU_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO5532683.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10700..12265)
                     /gene="ahpF"
                     /locus_tag="ACHKAU_003699"
     CDS             complement(10700..12265)
                     /gene="ahpF"
                     /locus_tag="ACHKAU_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO5532684.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12436..12999)
                     /gene="ahpC"
                     /locus_tag="ACHKAU_003700"
     CDS             complement(12436..12999)
                     /gene="ahpC"
                     /locus_tag="ACHKAU_003700"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO5532685.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13371..14117
                     /gene="dsbG"
                     /locus_tag="ACHKAU_003701"
     CDS             13371..14117
                     /gene="dsbG"
                     /locus_tag="ACHKAU_003701"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO5532686.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14326..>15107
                     /locus_tag="ACHKAU_003702"
     CDS             14326..>15107
                     /locus_tag="ACHKAU_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5532687.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLN"
BASE COUNT         3670 a         3946 c         3738 g         3753 t
ORIGIN      
        1 cacgccgaaa tactccttag gatgtatagc gaaaggagaa aaagatatac ctcgatcacc
       61 cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc aacattgaac aacattcgtt
      121 gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc aatgatattt
      181 tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg
      241 aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc cgcgatgaaa
      301 agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct atcagtgaat
      361 ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac ctcgcctatg
      421 agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg ctgttccgcc
      481 agtgcggttt ttccacgctg accagcgtac ccggcgtgat ggtgctgatg gaaaacagcg
      541 ccacgcggct gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca gggaacaaga
      601 ttggctgcat tgtgatgaac gccaatccct tcacgaatgg tcaccgttat ctgattcaac
      661 aagctgcggc acagtgcgac tggttgcatc tgtttttagt caaagaagat tcttcacgct
      721 tcccctatga agaccggttg gatctggtgt taaaaggcac cgccgatatt ccacgcctga
      781 ctgtgcatcg cggctccgaa tacatcatct cccgcgctac gttcccttgc tacttcatta
      841 aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt ttccgtcagt
      901 acctcgctcc cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg
      961 ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact atctccgcac
     1021 cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg atatccgctt
     1081 cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg ctggtccctg
     1141 cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac gcggcagctc
     1201 gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc
     1261 aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata cgcaggatat
     1321 cgacctgcaa atcaatagca gcgttgagaa acagtttggc gatgcaattc gcaccaccat
     1381 tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg atgacaaagg
     1441 cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg ccagcggtat
     1501 cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca acgtaaaact
     1561 cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt cagcaactcc
     1621 ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtggc attacgtgaa
     1681 aaagacaccg cccgccgcat ggtttatcac gcgctgcaac atccgctgta tcgcgatatt
     1741 gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg gtgttaacga cctggaagcc
     1801 gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt
     1861 ctggatattg aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga acccggcagc
     1921 accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt ggaaatcgct
     1981 cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg
     2041 cgtacagaac gctccccgga aggaactgaa ctgctgttcg cacgctgttc cattttgcag
     2101 gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc taacaacgaa
     2161 gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg caaatcgctg
     2221 atcaacccgc gtcagattga tctgttgcac aacctctacg caccaaccca gaaagaagtg
     2281 gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg
     2341 gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg tctggtgctc
     2401 tctcgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga cgcagaaaat
     2461 tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcgctg aatgcgatct
     2521 tgccgctttc cagaactcgc caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa
     2581 actgtgcgcc aacctggaag aagcgattcg tcgctctggt ttacaggacg gcatgacggt
     2641 ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga tggacgtcat
     2701 cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc tccctgagtg attgccatgc
     2761 gccgttggta gaacacattc gccagggcgt ggttacccgc atttatacct ccggcctgcg
     2821 cggtccactg gcggaagaga tctcccgtgg tctgctggca gaaccggtgc aaatccactc
     2881 tcacggcggt cgcgtgcatc tggtacagag cggcgaactg aatatcgacg tggctttcct
     2941 cggcgtcccg tcctgcgatg aattcggtaa tgccaacggc tacaccggta aagcctgctg
     3001 cggctccctc ggctatgcaa tggttgatgc cgacaacgca aagcaggtcg tgatgcttac
     3061 cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc aggttgattt
     3121 gatcgtcaaa gttgaccgcg ttggcgatgc tgcaaaaatc ggcgctggcg cgacccgtat
     3181 gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga ttgtcaactc
     3241 aggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgcat cgctggcggt
     3301 aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact tcgcccttgg
     3361 cggtattacc gcgacgatgg ttgacctgca cgaaaaaggt ctgatccgca aactgctgga
     3421 tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca atcacatcga
     3481 aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc gcatcggttg atcgtctcga
     3541 cgtggtggta ctgagcgcgc tggaaattga cacccagttc aacgttaacg tgctgaccgg
     3601 ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc gataccgcga ttgcctctgc
     3661 gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt ccgactctgg tggataacgt
     3721 gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc acggtatcgc
     3781 agttaacccg gcacgtccgg aactggcaga acgtctgcag gaagcgggca ttaaagtggt
     3841 ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac agccgattga
     3901 attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt
     3961 gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc accatgcggt
     4021 gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac acgtctggct
     4081 caagcgccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc cgattaaaga
     4141 cagcgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg ccttagccgc
     4201 aaaacagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg ggccggaggg
     4261 catgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg agcttgaaca
     4321 tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag gcgaaattct
     4381 ctcccgccgc gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc aaagcgcagc
     4441 cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca tggaggcact
     4501 gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa
     4561 tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga
     4621 aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg ctggaagatt
     4681 tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa tttggtgcct
     4741 gtagtgcaga aatggcaccg gaagcggtac tccacggatt acgcccaatt ggtatggctt
     4801 gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaaggc agcatttttt
     4861 ctttagggct gctatgtgcg gcaattggcc gtttgcttca gctcaaccag ccggtaacgc
     4921 caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg tctgaccgat cgcgaactgc
     4981 gtaccaataa ttcacaactg acggcaggtc aacggttgta ccagcagctt ggccttaccg
     5041 gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat caaccacgcc ttgccgcatt
     5101 acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat accttgctcc
     5161 tactgatggc gatcaacggc gataccaacg ttgcttcgcg cggtggcgag gggggcctgc
     5221 gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga acccccgccg
     5281 atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc agtccaggcg
     5341 gcagtgccga cctgctgatc cttacctggt ttttagcaca gatttaatta tttaagcact
     5401 tgataaattt gaaaatatta attttcggag aacccgtatg tctttagcaa aagataatat
     5461 atggaaacta ttggccccac tggtggtgat gggtgtcatg tttcttatcc ctgtccccga
     5521 cggtatgcca ccacaggcat ggcattactt cgctgtgttt gtggcaatga ttgtcggcat
     5581 gatcctcgag ccaattccgg caacagcgat cagttttatt gcggttacta tttgcgttat
     5641 tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt ttaatgcgca
     5701 aaaacaggcg ctgaaatggg gtctggctgg tttttccagc accacggtat ggctggtatt
     5761 tggcgcattt atttttgcat tagggtatga agtttccggg ttaggtcgtc gcattgccct
     5821 tttcctggtg aaattcatgg gcaaacgcac gctgacgttg ggttatgcga ttgtcattat
     5881 cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg gtacggtttt
     5941 tccggtcatt aaaaacctgc cgccgttgtt taaatcattc cccaacgatc cgtccgcgcg
     6001 tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga gttcgtccat
     6061 gtttgtcacc ggtgcggcac caaacgtgct gggtctggag ttcgtcagca aaattgccgg
     6121 tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg ttatcttgct
     6181 tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac acagtgaaga
     6241 agtggcaacc tgggcgggtg atgaactgaa aaccatgggt gcgctgacac gcagagagtg
     6301 gacactgatt ggccttgtat tgctcagctt aggtttgtgg gtttttggca gcgaagtcat
     6361 taatgctact gcagttggtc tgctggcagt ttcgctaatg ctggccctgc acgttgtgcc
     6421 ttggaaagat attacccgct ataacagcgc atggaacacg ctggtcaacc tggcaactct
     6481 ggttgtgatg gctaacggcc tgactcgttc tggctttatt gactggttcg ccggtaccat
     6541 gagtacgcac ctggaaggat tctcaccaaa cgcaacggtg attgtactgg ttctggtgtt
     6601 ctactttgca cactacctgt ttgccagcct gtctgcgcac accgcaacca tgctgccggt
     6661 tattctggcc gtcggtaaag gtattccggg cgtaccaatg gaacaactgt gtatcctgct
     6721 ggtgctgtct atcggtatca tgggctgtct gacgccgtat gcaaccggtc ctggggtgat
     6781 tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg caatcttcgg
     6841 ggtgatttac atctctatgt tgctgttggt tggctggccg attctcgcca tgtggaacta
     6901 atacgttcgc caaacgctga ataaatcatc acgcccgcca ggtaagccca cctggcgggc
     6961 ttttttatga tttaatagat agtattcagt gtatgtatga gttccacacc cattatgaaa
     7021 gcattctggc gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc ttctgccaac
     7081 gccttagcgt tgcaggcaaa acagtatggc gattttgatc gctatgtcct ggccctctcc
     7141 tggcaaaccg gattttgcca gagtcaacac gatcgaaatc gtaacgaacg agatgaatgt
     7201 cgcctgcaaa ccgaaacgac caacaaagct gattttctga ccgtacatgg tctgtggcca
     7261 ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat gcgcttcggt
     7321 tgcgctactc gcccaatccc gaatctacca gaagcgcacg ccagccggat gtgttcatcg
     7381 ccggaaaccg gattatcact ggaaacggcc gctaaactaa gtgaagtcat gccaggagct
     7441 ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg ctttggtttt
     7501 gatccggacg catacttcgg tacgatggta cgcctgaatc aagaaattaa agagagcgaa
     7561 gccggaaaat tccttgcgga taattacggt aaaacagtga gccgccgtga ctttgacgcc
     7621 gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac gtgccagggt
     7681 aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgcaat caacgctccg
     7741 ctttctgcaa actcattctt gccacaacca cacccaggca actgtggcaa aacctttgtg
     7801 attgataaag tgggttatta atcatctgat ttacaaaaca cgctaattcg tcttccataa
     7861 accgtgacga ttaagaatta tacataatgc gctgcattga ggaattccct gaatgtgacg
     7921 caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa ccctcgccgc
     7981 ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagt accgacgaca
     8041 tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg aacgcatcga
     8101 tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt taaacgcaga
     8161 gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg tgacaatgaa
     8221 cagccgggtt aaattccgca atcttagcga tggcgaagtg cgtgtgcgca cgctggtgta
     8281 tccggcaaaa atgaccgata gcaatactca gctttccgtt atggctccgg taggtgccgc
     8341 actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg gcgttgcaac
     8401 ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact acctgcttta
     8461 atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca tcctctttac
     8521 gcaaaatcag caatttttcc tggttctcac tgttcacttt tgctgcaaaa ttattccgtt
     8581 tcgtttgtgg gaataaccat ggcagaagca ttttacatcc tgataggatt tctgattatg
     8641 gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc actcctgacg atcatattgt
     8701 tccccccggc atcgcattca ccagacctga aaccgccttc tgcgccgcct ctgcgctttg
     8761 tgcaccgggt accagaatca ccttacggca ctcctcttca cgtttttcga aaatctcata
     8821 tccccgggcg gcctcttcaa acggcatata gtgggtaaca atttcttctg gtttcagtaa
     8881 tcctttctca attaacggta ataattctcc cagccatgcg tgaacgtggg tctgtcccat
     8941 tttaaacgtc aaccctttat caaaggcgtc gccaaacagg aaaccgtgaa taaatccagc
     9001 gtagacgccc ggtacgctaa caatgccgcc acgcctgacc gccgcaatac actggcgcaa
     9061 cgctttaccg ctgctgccct ccagtttcag gttagtcagc accgtttccg tggtgctgcc
     9121 tttcgcttca aaaccgacgg cgtctattac tgcatccacg ccccggtgac ctgccgtttg
     9181 ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa ttaatcggga tcgcgccgta
     9241 gcggtcagcg gcgaaacgca agcggtaggg atgatgatca acaacaaaaa tctgttccgc
     9301 accgagcaac cgtgcacagg cgattgtcaa caatcccact ggaccggcac catagactgc
     9361 aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc tgccatgccg ttggcagaat
     9421 atcagaaagg aaaagggctt tatcatctga aagcaaaggc ggtactttaa acggccccac
     9481 attcccttta gggacgcgga catattccgc ctgtccacca ggaacgccac catacaggtg
     9541 actataacta aacaatgccg cgggagctgg tatctgtttt ttattgagcg cagcgccttt
     9601 acccgcattg gtattttcgc aggcggcata ttgctgcatt cgacagaaaa aacagtcgcc
     9661 acaagcaatg acaaacggaa ttaccacccg gtcgcctttt tgcaaatttt ttacgtcctt
     9721 tccggtttca acgacttccc ccataaattc atgaccaaaa atatcgccat gtttaacctg
     9781 gggtattttg cctcgataaa gatggaggtc agagccacag atcgccgttg ccgtaatacg
     9841 cagaataata tcatctgcct gttcaatgcc cggatcggga acattttcta cctgaacgtg
     9901 atgtgggccg tgataagtca atgctttcat gcaacctcca gagacagttc aaaaaagagt
     9961 cagtctttaa gggtagctat cacagcggga aaacgggcgg taccgcaggt tttgggagaa
    10021 ttcctggtgt tcagtgagaa tatattaatc acagaaaccg ttcgtaagga aaaagaacca
    10081 gacaaatatc agggataaat attataatca tggcgaatga tttgttcatg attaacaggg
    10141 agaaagggtt atgtataaga caatcattat gccagttgat gtatttgaaa tggaattgag
    10201 cgacaaagct gttcgccacg ctgaattcct cgcccaggat gacggagtta ttcatctact
    10261 tcacgtacta cccgggtcag ccagcctgag cctgcaccgt tttgccgctg atgtgcgtcg
    10321 ttttgaagag catctgcaac atgaagcaga agaacgtctg caaacgatgg tcagccactt
    10381 caccatcgat ccttcccgca ttaaacaaca tgtccgtttt ggtagcgtgc gggatgaagt
    10441 caatgagttg gcagaagaac tgggggctga tgttgtagtt attggttctc gcaacccatc
    10501 gatttcgacc catctgttag gttctaacgc ctcgagcgtg atccgccatg ccaatctgcc
    10561 ggtgctggtt gtgcgttaat tgccagaata aatatcccgc cctgcccgga gccatcccgg
    10621 cagggctttt taatgccata aaaaagccgc caggtttgac cccggcggct aagcaattgc
    10681 aggtgaatct tacttcttct tatgcagttt tggtgcgaat caggtagtca aaagcactca
    10741 gagaggcttt ggcaccttcg cctgtggcga tgataatctg cttgtacgga accgtcgtac
    10801 agtcacccgc tgcgaacacg cctttgacgt tggtttcgca tttcgcatca atgataatct
    10861 cacccatgcg gttacgttcg actgcgcctt ccagccagtt ggtgttcggc agcagaccaa
    10921 tctggacgaa aataccggcc agttcgatgt tgtgaatatc gccgctgaca cgatctcgat
    10981 attccagacc aacgactttg ctgccgtcgc ctttcacttc cgtggtttgc gcattcagaa
    11041 taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag aacctggtcg gctttcattt
    11101 ctggcgcaaa ttcgagcagc gttacgtgct caacgatacc cgccaggtca attgccgctt
    11161 ccacgccgga gttaccgccg ccgataaccg ctacgcgttt gcctttaaac agcgggccgt
    11221 cgcagtgcgg gcagtaggtc acgcctttgg tgcgatactg atcttcgcca ggaacgttca
    11281 tgttgcgcca ttttgcaccg gtcgccacga taatgctgcg tgctttcagt accgcgccag
    11341 aagctgtttc aatctgatgc agaccacctt caactgctgc tgggatcagt ttgctggcgc
    11401 tctggctgtc gatcacatca acgtcgtatt catcaacgtg aactttcagt gcgcctgcca
    11461 gcttctgccc ttcagtcttc ggtacagaaa tgtagttttc gatatcaacg gtatcgagga
    11521 tctgaccacc aaaacgttcg cccatcagac cggtacggat gcctttacgt gcggagtaaa
    11581 ttgctgcagc tgcacccgcc gggccggaac caacgattaa tacgtcataa gcatcacgct
    11641 tgttcagctc ttctgccgca cgtttttccg cgcccgtatc aattttggca acgatttcag
    11701 tcaacgtcat gcggccctga ccaaactctt tcccgtttac gaacactgcc ggaacgccca
    11761 tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc gtcaattgca gtgtgcttga
    11821 tgcgcgggtt cagtacgctc atcaggttca gcgcctgcac cacgtccggg cagttatggc
    11881 aagagagcga gtaataggtt tcgaattcaa aatcaccgtc aatatggcga atctgctcca
    11941 gcagagactg cgcttctttc gacggatgac caccggtcca tagcaacgcc agtaccagcg
    12001 aggtgaactc gtggcccagc ggggagcctg caaaacgtgg cccctggttg gaacctgggt
    12061 tggtgatcag gaaagacggc ttacgcaccg gcaagctgtt atcttcttta aaggtgactt
    12121 tgtctgacag ttctgcgatt tcagccaaca gttccttgat ttctgccgat ttagcgctgt
    12181 catccagcgt ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa gccttgagtt
    12241 gagttttcat atttgtgtcg agcatgttta tctcctgggc tttaaacatc atcatgcaag
    12301 cggcctctgc ggctacctgg atgcagcttg catcatggtg cggggaagaa aatcgggtgc
    12361 cgcagcaccc gaagaattag gtgaatttcc gaagtatttc gcgccggagt tggtagcgcg
    12421 aaagacgaag gaaatttaga ttttaccaac caggtccaga gacggagcca gagttgcttc
    12481 accttctttc catttagccg ggcaaacttc acctgggtga gaagctacgt actgtgctgc
    12541 tttgatttta cgcagcaggt cagacgcgtc acggccaatg ccttcagcgg taacttcgat
    12601 tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca cggtcagcca gaccttcatc
    12661 ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc gggtcgccga tcatcgcata
    12721 tttgatttta gcgatggttt cagagctgct gtgccatgct ttgtgggtga agtgagtatc
    12781 ggtagatact gcgtatacgt ctacgcccag tttctgcagt tcttcgtagt ggtcagcaac
    12841 gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc gggtagaaga agaagacgct
    12901 ccagcggcct tcggtatctt tttcggtgat ttcgatgaat tcgccgtttt tgaatgcctg
    12961 gtttttaaaa ggtttaattt tggtgttaat caaggacatc tatacttcct ccgtgttttc
    13021 gatgagatgt aaggtaacca atttttgccg attcggctaa tgcgtttcca ttatcaaatc
    13081 gataagtttt accttacaac cttcgtaaga caactttgtg aactttgcac cgccagcggc
    13141 gataaaaagt aaaatggccc cctttccagg tgaccgtgta atctgaacta ccctttggta
    13201 tcttcagtgc ctgcctgcag gctgaggtgt tgcccttaca aatgcaacaa cgacatggat
    13261 tacaacaccc tcataaacaa agggcaatca cctgatctaa gctcttacct atgacagtga
    13321 taggttatgc cttttactcg acttttgcac tgactgaaaa ggacaaatta atgttaaaaa
    13381 agatactttt actggctctg cttcctgcaa tcgccttcgc agaggaactt cctgctccag
    13441 taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac attcgatgcc cccggaggaa
    13501 tgaaaggtta tctcggaaag tatcaggata tgggcgtcac catctacctg actccagatg
    13561 gtaagcacgc tatctctggt tacatgtaca acgagaaagg tgaaaacctg agtaacacac
    13621 ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat gtggcaacgg atggaacaat
    13681 cccactggct cctcgacggt aaaaaagatg cgccggtcat tgtctacgtg ttcgccgatc
    13741 cgttctgccc atattgtaaa cagttctggc agcaggcgcg cccgtgggta gattctggca
    13801 aagtgcaatt aagaacattg ttggttgggg ttatcaagcc agaaagcccg gcgacagcag
    13861 cggcaattct tgcctccaaa gatcccgcaa aaacctggca agaatatgaa tcctctggtg
    13921 gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga acaaatgaaa gtgttaagcg
    13981 ccaacgagaa actcatggac gatctggggg caaatgtcac accagcgatc tattacatga
    14041 gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga tcagaaaacg cttaatatca
    14101 ttatggggaa taaataaaag ataattcatg gatggttgaa atacatccct gcttttcaac
    14161 catcctaaaa aagcatgaat tgctcgctct gccctaagtg gctatgctat tttagaaaaa
    14221 cttaatcctg agttaagcaa aaaaatataa tcaataaaaa tatatacgca aaaaacattg
    14281 attaagtgaa tataccatgg aagaaaaata taaccggagt agtgtatggc caatctctac
    14341 gacttgaaaa agttcgactt aaaccttctt gtcatatttg agtgtattta ccagcattta
    14401 agcattagca aagctgctga atcactatat atcaccccat cagccgtgag ccagtcgtta
    14461 caacgcttac gtgcgcaatt taatgaccct ttattcattc gctcaggaaa aggtatcgcg
    14521 ccgacgacaa cagggttgaa tctgcatcac catcttgaga aaaacctcaa aggccttgaa
    14581 caaacaatta atatagtcaa taagtccgaa ctaaagaaaa actttattat ttacggccct
    14641 cagctcattt cttgctctaa taatagtatg ctgatccgct gcctacggca agatgcctct
    14701 gtcgagattg agtgccatga tatcctcatg tcagcagaga atgctgaaga actgttggtt
    14761 caccgcaaag ccgatctggt gatcacccaa atgccagtga tcagccgttc cgtcatttgc
    14821 atgccattgc atacaattcg caatactcta atttgtagta ataagcatcc gcgaattact
    14881 gacaatagca cttatgaaca aattatggca gaagagttca cccaattaat atccaaatca
    14941 gcaggagttg atgatattca aatggaaata gacgagaagt ttatgaatcg caaaatttca
    15001 tttcgtggtt cctctttgct gacaatcatt aatagcattg cagttacaga tttactggga
    15061 atagtcccct atgagcttta taattcctac cgtgactttc ttaattt
//
LOCUS       DAZFJF010000106        15093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFJF010000106 DAZFJF010000000
VERSION     DAZFJF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.106"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(71..146)
                     /locus_tag="ACHKAU_003703"
     tRNA            complement(71..146)
                     /locus_tag="ACHKAU_003703"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Phe,seq:gaa)
     gene            complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHKAU_003704"
     CDS             complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHKAU_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO5532688.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHKAU_003705"
     CDS             1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHKAU_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO5532689.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            1357..3492
                     /gene="speC"
                     /locus_tag="ACHKAU_003706"
     CDS             1357..3492
                     /gene="speC"
                     /locus_tag="ACHKAU_003706"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO5532690.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHKAU_003707"
     CDS             complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHKAU_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO5532691.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHKAU_003708"
     CDS             complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHKAU_003708"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO5532692.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHKAU_003709"
     CDS             complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHKAU_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO5532693.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHKAU_003710"
     CDS             complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHKAU_003710"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO5532694.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7660..8379
                     /gene="trmB"
                     /locus_tag="ACHKAU_003711"
     CDS             7660..8379
                     /gene="trmB"
                     /locus_tag="ACHKAU_003711"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO5532695.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8379..8705
                     /gene="yggL"
                     /locus_tag="ACHKAU_003712"
     CDS             8379..8705
                     /gene="yggL"
                     /locus_tag="ACHKAU_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO5532696.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8889..9608
                     /gene="yggN"
                     /locus_tag="ACHKAU_003713"
     CDS             8889..9608
                     /gene="yggN"
                     /locus_tag="ACHKAU_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO5532697.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9784..10830
                     /gene="ansB"
                     /locus_tag="ACHKAU_003714"
     CDS             9784..10830
                     /gene="ansB"
                     /locus_tag="ACHKAU_003714"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO5532698.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10947..11954
                     /gene="yggM"
                     /locus_tag="ACHKAU_003715"
     CDS             10947..11954
                     /gene="yggM"
                     /locus_tag="ACHKAU_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO5532699.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12010..13311)
                     /locus_tag="ACHKAU_003716"
     CDS             complement(12010..13311)
                     /locus_tag="ACHKAU_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO5532700.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13311..13814)
                     /locus_tag="ACHKAU_003717"
     CDS             complement(13311..13814)
                     /locus_tag="ACHKAU_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO5532701.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13859..14845)
                     /locus_tag="ACHKAU_003718"
     CDS             complement(13859..14845)
                     /locus_tag="ACHKAU_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO5532702.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         4020 a         3599 c         3797 g         3677 t
ORIGIN      
        1 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
       61 tatatgaatt tggtgcccgg actcggaatc gaaccaagga cacggggatt ttcaatcccc
      121 tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc gcctcgtctc
      181 gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact ttgccgctat
      241 tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat tgccagaagg
      301 agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg taatccggtt
      361 gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc
      421 ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa tgggatacta
      481 attaccgaca ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga
      541 aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat cgccccgaag
      601 atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg aataaaagat
      661 tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggc gactgctgta
      721 ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag cgtagctaaa
      781 accatcgctg gaaggttggc gcatttcaca accagtaaaa taccaatccc cagcgatgcc
      841 agaccaaaaa tagatgtcat ggagacgcgg atacgttccg gtaagcgttg gctgagcagt
      901 gcgccgagaa cgccacccag taagactgca ctagcgttga taaaagggcc gatgaccaca
      961 agagttcctg ttagctgtat tgtttgattt tcttattatg aactttttgc cttgttgatg
     1021 gcttgtctgg tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca tgattgcgcg
     1081 aattttctcc tctctgtacg gagtttgccc gatgcacgcc atctccttac attctctcgc
     1141 ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc ggtgaagtta
     1201 acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg
     1261 gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt tccaccttgt
     1321 ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa tattgccgcc
     1381 agtagtgaac tggtatcccg actttcttct catcgtcgcg tggtggcgtt gggagatact
     1441 gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag tggcattctt
     1501 gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga acatgctgtt
     1561 gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg gctggagctg
     1621 gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga cacgctgacg
     1681 cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca tggtgcgttt
     1741 tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa cgtctttcgc
     1801 gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga aggatcggcg
     1861 aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta ttttgtgctg
     1921 aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg tggcgatctg
     1981 gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat tcaggcgggg
     2041 gcgacgccgg tctatctgga agcttcacgc aacccgtttg gtttcattgg cggtattgat
     2101 gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa
     2161 gccgagctgc cgcgcccgtt tcgcctggcg attattcagc tgggaaccta tgacggaact
     2221 gtctataacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta cattctgttt
     2281 gattccgcgt gggtcggtta cgaacaattt atcccgatga tggcggatag ctcgccgctg
     2341 ctgttagaac ttaacgaaaa cgatccgggg atttttgtga cccagtcggt gcacaaacag
     2401 caggctggat tctcacagac gtcgcagatc cataaaaaag ataaccatat ccgcggacag
     2461 gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc ttctaccagc
     2521 cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga aggggagagt
     2581 gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa ggctattctt
     2641 gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa attgtggcag
     2701 gattatccga catctgtgtt agccagcgac cgccgttttt tcagttttga gccgggggcg
     2761 aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc gtgcaagctg
     2821 ttactcacta caccaggtat caatgccgaa actggcgaat atagcgactt tggcgttccg
     2881 gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa gtgcgatctc
     2941 aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc acaactggtg
     3001 gcgatgctgg cgcaatttga acagcatatt gaggatgact cgccgctggc tgaggtgttg
     3061 ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg ccagttgtgt
     3121 caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc
     3181 cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag cgcttatatt
     3241 cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc ggcagaaggg
     3301 gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg gggtggggcg
     3361 gttcaacgtt atttccttgc actggaagaa ggggtgaatt tgttgccggg attttcgccg
     3421 gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt gtacggttat
     3481 gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt tctttgcgta
     3541 attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat ttgaacatcg
     3601 ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat acggtctgcc
     3661 agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg atgccgccga
     3721 ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg gcgcgaattg
     3781 ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag tcgaatgcgc
     3841 aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc gggtcgccgt
     3901 aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt acgttcttaa
     3961 taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca gagatctgcg
     4021 aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac atcggatctt
     4081 tgtcgaagct gtgcaggaag gtattaccga acatattggt aatctgcagt tccgcgccca
     4141 gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc gcgaatgcat
     4201 cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc ggaatatgcg
     4261 gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata tacagctgca
     4321 tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata aagccgatgg
     4381 tgccccagat acggattggc gggaagtcag taacgatatc catcccggca ttttgcaggc
     4441 gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac gagttaatca
     4501 atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac agcgtgatag
     4561 cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg tcggccacaa
     4621 tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa actgcgccaa
     4681 tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc gtcagccaac
     4741 ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta agattcatgt
     4801 taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga ccgtgcgaga
     4861 tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa ataattgcaa
     4921 acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc ctgagcatcg
     4981 tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta ttcactttat
     5041 aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga taaacatcgc
     5101 ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa aagactcgca
     5161 gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc gatgttgggt
     5221 tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg ccggtatcaa
     5281 tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc ccctgcgagc
     5341 ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc gcatcggaac
     5401 ggctgaccgc atatgggtta aaggaagatt cggtctgcat aatcgccaga atcagcgact
     5461 catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg tgcgcacgtt
     5521 tatcaaggtg gttcggcacc atgttaatgg taacgctgta gatgatacgc agcccgttgc
     5581 tgcggctctt cagacggttt ttcagcagat aatccgcgaa gttgctcgcg cgaccttccc
     5641 agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt tctttcgaaa
     5701 tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca cccatcagca
     5761 acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg atagtttcga
     5821 tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga tcggtgtatt
     5881 tcacgtagtc cttaggaccg gcgatcacca cctctttgaa tccccagatg ttctcaatat
     5941 tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc caggcttcgt
     6001 tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca atcaaagcca
     6061 gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat gcaagttgcc
     6121 ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc ctcgatatgc
     6181 acctctttac cctcgaacag gaagttgacc atctcctgct ccagcagctt gcggtgctcg
     6241 gcattcatca tgttgagttt cttttcatta atcagcatgg tttgtttgtg ctgccactgc
     6301 gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc ggggtacagc
     6361 tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt tctgctcata
     6421 aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa ctgctgtaac
     6481 aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt ataccagagc
     6541 gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat aggcacaata
     6601 tctaagtgga aatggctgaa ggtatgccga aacgcggtca gctgcgtcag gttatcggca
     6661 gcaatctgcc gttgcgccag ccactgccgc aaactttctt cgtcggcaaa ctgcgggaaa
     6721 cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac ttcatcttcg
     6781 tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg ttttttgccc
     6841 ggataaagcg cccagctatt gttggcggcg gcaatacatc cgttttgtag cggacagagc
     6901 gaacatttcg gtttcgagcg cgtacaaatc atcgcaccca aatccatcat cgcctgatta
     6961 aaccgttcca cgccaaccgc gggcgtcacc tgctcgctca aactccataa tttattctcg
     7021 acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac ccgtttgacg
     7081 ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc acctgcggtg
     7141 gaacgcccga cgcccggtaa cgccgcgact tcttcaaagg tttccgggaa tttaccgccg
     7201 tgtaaggtcg ccacttgttg tgccgcttta tgcaggttgc gcgcgcgggc gtaatagcca
     7261 agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc ggtcaccgtc
     7321 gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg agtttgttgc
     7381 aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca gggcagcgtt
     7441 ttccgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga cgcttgcatg
     7501 gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca gggtgtaaac
     7561 cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac tttggataat
     7621 gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga cgtcatttca
     7681 ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt gcgccgccag
     7741 gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt gatgggcgtt
     7801 gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc gccggtgacg
     7861 cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga tcgccctgag
     7921 caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct ggcttctgcg
     7981 catgaagaag gtttaagcaa cctgcgcgtg atgtgtcacg atgcggttga agtgctgcat
     8041 aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga cccgtggcac
     8101 aaagcgcgcc ataataaacg ccgtatcgta caggtgccgt ttgccgaact ggtaaaaagc
     8161 aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta tgcggaacat
     8221 atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag caatgattac
     8281 gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca tcgtcttggt
     8341 cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac cgtagccgtc
     8401 gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg gtggcatggc
     8461 gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat tttattaacg
     8521 aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc tgggaaggtc
     8581 tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt
     8641 ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac gtttggtggg
     8701 actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta cgcttatcag
     8761 gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca tccggcatgg
     8821 aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt ttttcttgag
     8881 ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg gcaatgaccg
     8941 ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa
     9001 ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc aacgtgatgt
     9061 ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag gattatcagg
     9121 ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc gtcgaaaaag
     9181 cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc aaaatgcgca
     9241 gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt atcgaaacgc
     9301 gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc gaaggccagc
     9361 aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa atgggcgcga
     9421 aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc ctgggggggc
     9481 tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag cagtttggca
     9541 aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg gtcgggaatt
     9601 taaaataatc ctctatttta agacggcata atactttttt atgccgttta attcttcgtt
     9661 ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta aattgtttaa
     9721 cgtcaaattt cccatacaga gctaagggat aatgcgtagc gttcacgtaa ctggaggaat
     9781 gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt tagtggtgca
     9841 gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc cggtggtggt
     9901 gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat
     9961 gcggtgccgc aactaaaaga cattgcgaac gttaaaggcg agcaggtagt gaatatcggc
    10021 tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa caccgactgc
    10081 gataaaaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga aaccgcttac
    10141 ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg
    10201 tccacgtcca tgagcgcaga cggtccattc aacctgtata acgcggtagt ggccgcagct
    10261 gataaagcct ccgctaatcg tggcgtgctg gtggtgatga acgacaccgt actggacggt
    10321 cgcgatgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt taactacggt
    10381 cctctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc acgtaagcac
    10441 accagcgata cgccattcga tgtctctaag ctgaatgagc tgccgaaagt cggcatcgtt
    10501 tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc gggctatgat
    10561 ggcatcgtta gcgctggtgt gggtaatggt aacctgtata aatccgtgtt cgacaccctg
    10621 gcaaccgccg cgaaaaacgg cactgcagta gtgcgttctt cccgcgtacc gacgggtgct
    10681 accactcagg atgctgaagt ggatgatgcg aaatacggct tcgtcgcctc tggcacgctg
    10741 aacccgcaaa aagcgcgcgt cctgctgcag ctggctctga cgcaaaccaa agatccgcag
    10801 cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt gccggggctt
    10861 tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa tcaccggaat
    10921 aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc acggcactgc
    10981 ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa aatatcttaa
    11041 aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc accttaaaaa
    11101 atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc tcttccagtg
    11161 acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa cagacctgga
    11221 cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg cccgcgtctg
    11281 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
    11341 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattatcgct
    11401 ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg ctggaaaagg
    11461 aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat gcatattggg
    11521 gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa attcaccaac
    11581 agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag
    11641 aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt tatgaagtgc
    11701 cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacagact tttctgcaat
    11761 cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gcttgaaaaa gggcaatatc
    11821 cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg atgaaaattg
    11881 acgatatcaa ccaagctaat gaacgctgga aaaaagatac cgaacaactg cggcgcaatg
    11941 gggtgattaa gtaagtttga tatagtgcct ttgccggata atataaaaag ttatccggca
    12001 aattattttt tacattagcc ctagtagctg tggaatgaac aaggatattt gcggaatgta
    12061 agtgatcatc atcaacgcta ctagcaatgc agcataaaat ggcagcaatg gtttgattaa
    12121 atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac ccaccggcgg
    12181 tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta ctgggtccat
    12241 tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag ccggtgtcat
    12301 gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata tgatcagtgg
    12361 gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag cattagtcat
    12421 tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag tggtaacaac
    12481 cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa ccagcaccga
    12541 gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa aaatgccgcc
    12601 cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc
    12661 tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc gtttagcaat
    12721 aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat atcccgccat
    12781 aaatagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta acacgtttga
    12841 gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag aaaatgccgg
    12901 atcataacct tgtttggtct gaatgggatt cagtgtgcca ccaacggcag cagctgccgc
    12961 aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca catgaccaag
    13021 tgaaccagga acccgaccaa ccattacctg ggcgaggtta atcaaacgta tcgcaatacc
    13081 gccgctgttc atcagcgttc cggcaaagat gaaaaagggg attgccaata gggcaaagtt
    13141 atccagtcca ttagccagtc gctgcgaaac agtgattgcc gcgatatccc agggaaacat
    13201 caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca caccaataaa
    13261 gaccaacaca aaaaaacagc caaacagtgt aatcgcgata taactatcca ttaactggcc
    13321 tcctgcgcat gttgtttcag ttgttgaatg ctttgattaa taaaacacaa ggcataaaac
    13381 atcatcacca atccacttaa tggcagaatg atatatacgt atcccatcgg tatttgcatc
    13441 gcggcagata cctgtgatgt tgccagcgtt ttatcaatta atttcaggcc accagtcaca
    13501 atgaccgaac cggcaaaacc taaaataaga acgttaatga ctatactaag aagtaattgc
    13561 ttgcgcttat tcagggcgag agcaaataaa tcgatagaga gatgtctctg cgcgccgacg
    13621 gtatatgcag cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt
    13681 aatgtacttg gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc
    13741 agagcaagca tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg
    13801 cggttaaata tcattgtaag catcctggaa aatcagggcc agagactggc ccggaatatt
    13861 attgagcagc gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt
    13921 cgtcaaacag aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac
    13981 cccccatagc tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt
    14041 gataagcttc ggatttcttc gctgctgttt cgaggatttg ttgctgttct ggggttaatt
    14101 tattccatgt tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag
    14161 aaaaaacttt ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg
    14221 cgccatctac tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg
    14281 gtgaaccacc catcagttca atcattttga tggtggtcgg acttggctga acacgaattt
    14341 tcagcccttt aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac
    14401 ccgctacata agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt
    14461 taccaacttc accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca
    14521 aattatagat agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca
    14581 tatccagagc gccattttgc aacaactcca tagtttctcg cgcattaccc atctggctgc
    14641 tgggataaat acggatcacc atattacctt ttgacagctg ttttacctct ttcgccagct
    14701 cttcaaaact ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca
    14761 ctttttctgc ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg
    14821 agcgggtaaa tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg
    14881 atatctaatt gtgcttatca gtgctttgtg cgacaaattg gttaacctgt ctgacaactt
    14941 gtctatgttt atcacaactt gaaataaagt cagaaattaa atattaaatt tgtgcgccag
    15001 gttgtacatg tttataatca ataaatgacg agtaaatatt cactatatat ataataaata
    15061 tcgtcgatat ttattcagta agaaatatat ttt
//
LOCUS       DAZFJF010000107        14480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFJF010000107 DAZFJF010000000
VERSION     DAZFJF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.107"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            104..460
                     /locus_tag="ACHKAU_003719"
     CDS             104..460
                     /locus_tag="ACHKAU_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5532703.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(511..1407)
                     /gene="yhaJ"
                     /locus_tag="ACHKAU_003720"
     CDS             complement(511..1407)
                     /gene="yhaJ"
                     /locus_tag="ACHKAU_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5532704.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1512..2213
                     /locus_tag="ACHKAU_003721"
     CDS             1512..2213
                     /locus_tag="ACHKAU_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO5532705.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2236..2400
                     /gene="yhaL"
                     /locus_tag="ACHKAU_003722"
     CDS             2236..2400
                     /gene="yhaL"
                     /locus_tag="ACHKAU_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO5532706.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDETTA"
     gene            complement(2612..3922)
                     /gene="cyuA"
                     /locus_tag="ACHKAU_003723"
     CDS             complement(2612..3922)
                     /gene="cyuA"
                     /locus_tag="ACHKAU_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO5532707.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3950..5281)
                     /locus_tag="ACHKAU_003724"
     CDS             complement(3950..5281)
                     /locus_tag="ACHKAU_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5532708.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5556..6920)
                     /gene="tdcG"
                     /locus_tag="ACHKAU_003725"
     CDS             complement(5556..6920)
                     /gene="tdcG"
                     /locus_tag="ACHKAU_003725"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5532709.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6992..7381)
                     /locus_tag="ACHKAU_003726"
     CDS             complement(6992..7381)
                     /locus_tag="ACHKAU_003726"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO5532710.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7395..9689)
                     /gene="tdcE"
                     /locus_tag="ACHKAU_003727"
     CDS             complement(7395..9689)
                     /gene="tdcE"
                     /locus_tag="ACHKAU_003727"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO5532711.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9723..10931)
                     /gene="tdcD"
                     /locus_tag="ACHKAU_003728"
     CDS             complement(9723..10931)
                     /gene="tdcD"
                     /locus_tag="ACHKAU_003728"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO5532712.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10957..12288)
                     /gene="tdcC"
                     /locus_tag="ACHKAU_003729"
     CDS             complement(10957..12288)
                     /gene="tdcC"
                     /locus_tag="ACHKAU_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO5532713.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12310..13299)
                     /gene="tdcB"
                     /locus_tag="ACHKAU_003730"
     CDS             complement(12310..13299)
                     /gene="tdcB"
                     /locus_tag="ACHKAU_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO5532714.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13398..14336)
                     /gene="tdcA"
                     /locus_tag="ACHKAU_003731"
     CDS             complement(13398..14336)
                     /gene="tdcA"
                     /locus_tag="ACHKAU_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO5532715.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3765 a         3882 c         3372 g         3461 t
ORIGIN      
        1 taaatatcaa aacctttata acatccgaaa aagaaagata cgaatagcat ttacacaata
       61 cccggaatag actttcgtca cttttaatta aagggatgtt tttatgcagt ggtatttagc
      121 cgtattaaaa aattatgttg gtttctctgg tcgcgcacgt cgtaaagagt actggatgtt
      181 tactctgatt aacgccatcg tcggcgctat tatcaatgtc attcaattga ttttaggttt
      241 agagtttcca ttcctttctc tcatttacct ggcagcaaca atcattccgg tcattgctct
      301 gtgtgttcgt cgtctgcacg atactgaccg ttcaggtgcg tgggcgcttc tctatctggt
      361 accgatcatt ggctggttag ttctgtttgt atttgcctgt ttagaaggca attctggcag
      421 caatcgttac ggaaacgatc ctaagtttgg ttcaaattaa tcttagaatt ggggcgacat
      481 ttcgcccctt tttattaata ataacaaata ttattttccg ctaaaaagtt tgggaatttc
      541 ccgcagacac caggatttcg cttcccccat actgtcacga cgccaggcca taataatatc
      601 gatctcgctg gtcgattccg ggctgacgac acgcaaccgc ccttccgcaa tatctttttc
      661 gaccatcgga tacggcatcg tcgccacgcc aagccccgcc agtaatgcct gacgtttatc
      721 ttcaatcgtg ctcaccgtta agcgcggctg tttgtccagc agctgtacgg tcaacaccgg
      781 gcgctcacga gcggtatccg ctaccgcaat tccacgatat ttcacgcgcg tcacttcaga
      841 taacggttcc ggctcctgat gaatcgggtg atcaggcgcg gcaacgtaga cgttcattaa
      901 cgtatagagc ttgcgcgagt tgatctccga cgaggaacga aaatgcatat ccggcgcgat
      961 aacaatatcc gcccgcccct gctccagtcg ttcccacgcc cccgccagca cttcggtgat
     1021 gattgccagt tgggtattgg cttttgccgc cagtttgtcg attaacggga aaaaggcagg
     1081 tgtcggtacc agcgcttcgg tcacaatggt gagatgcgtt tcccaaccgc gcgcgagggc
     1141 ttccgcatcg gtagtcagtt tatctgcggc ttccagcaaa acgcgccctc gctccagcaa
     1201 catcctcccg acattggtga atttggtgcg atggcccgag cggtcaaaca gcaccacatc
     1261 cagctcttct tccagttttt gcatggtgta gctaagtgca gaaggcacgc gtcccagctc
     1321 atccgccgcc gccgcaaaac tgccccggcg atcgatcgca tccataaccc gtagtgcttc
     1381 cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg aacataccgg
     1441 gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga agtaaagaga
     1501 ggtcaagaat tatgattact acccgaactg ccaggcagtg tggacaagca gactacggat
     1561 ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa ttgttaggct
     1621 atgcctccct gcgtgtgctt aaccaggaag tgctggcccc agatgccgcc tttcagccgc
     1681 gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagca gagtatcgcg
     1741 acagcgaagg caatcatgtt caggccagcg ccggtgaggc gttgctgctc tctacccagc
     1801 cgggtgtcag ctacagcgaa cacaatctca gcaaagacaa accgttaaca cgaatgcagc
     1861 tttggctgga cgcctgcccg cagcgagaga atccgctgat tcaaaaactg gcgcttaata
     1921 tgggcaagca gcaattaatc gcctcgccag agggtacgat gggaagcctg caattacgcc
     1981 agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat ttccagttgc
     2041 atgggccacg cgcgtatttg caatcgattc acgggaaatt tcatgcgctt acgcatcatg
     2101 aagagaaagc ggcgctgacc tgcggtgatg gggcgtttat tcgtgacgag gctaacatta
     2161 cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc tagttgtttt
     2221 tacagggaga tgatgatgag taaaaaatcg gccaaaaagc gccagccagt gaagcccgtg
     2281 gtggcgaaag aacctgctcg caccgccaaa aattttggct atgaagagat gttgagcgag
     2341 ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg aggatgaaac taccgcgtaa
     2401 agacaggttg ccggatgcgg cgaaaacgcc ttatccggcc tacaaaatcc aacaacatta
     2461 acgaattaaa caacccgtag gccggataag atgcgccagc atcgcatccg gcatcaggta
     2521 caaaacacaa caacatcaac aagttgagca ccccgtaggc cggataaggt gcgccagcat
     2581 cgcatccggc acgataccca aaacctggcg tttatctggc cttgctcgcc ataatctcga
     2641 taatctgccg atccgtttgc tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg
     2701 actgctcaac atcatgcgcc acgatccctt cattgccggt cacggcggta tcatccagcg
     2761 ccattaacac cgctttccac gcagccgaag cactggtcga aaccttcatc gcgcagctgt
     2821 tcgacgcacc atcgcaaatc atgccgctga catcgccgat catactgctg atcgccatcg
     2881 agatggtttc ataacgccca tccaccagcc atgccatccc ggcggcggcc cccattgctg
     2941 cggtcgttgc ggcacacagc gcagacaaac gcggtaactg gttatggatg taaattgcgc
     3001 tcaaatgaga aagcatcagc gcacgcgcca gccgttcatc atccgctccg aagtgttctg
     3061 ctaccaccac cacaggcatt gttgcggtga tcccctggtt acccgagccg gagttactca
     3121 tagccggaag cgtagcgccg cccatacgcg catcggatgc cgcgctggta cgaatcacaa
     3181 tggatgaaga gagatctttc gccagcaaac cgcgctcgca ctgtttttcc agcgtcgcgc
     3241 caatatgcag cccccactta ccgctcaaac cttcctgcga taacgcacaa tttagcttcg
     3301 cggaatcgag aataaagcgg atcgccgcaa acgggacttc attgacgaac ttcaggatct
     3361 cagccagcgt cgttctggaa agcaccgtca gcggagactc ttgctcgccc tctgccacac
     3421 acgcctgctg ggtaaacacc acaccattgt gcgtctcaat atgcacaatg ttggtatgcc
     3481 cgccgacgat ggtgacacac gcccacttct caccgttcca gactttggcg cgtgagaaga
     3541 ggatttcatt gcaaggttcc tggatcttaa cggagacttt ccccgccgcc agcagtgctt
     3601 tggcatcggc aattgcctgc gcagttgcgt ctttcagcac ttccagcccg gcgttggcat
     3661 ttccacctaa cgcccccagc gccgccgcaa tcggcagccc caccattccc gtgccgggaa
     3721 cggtgacgcc cagaccgttc ttcatcagat ttggcgaaac ccaggcttct acacgttcaa
     3781 ccggaccttc cagttctgcc gcagcaaccg ccgccgccag cgccagtgaa atcggttcag
     3841 tacatcccag cgccggtttt acttcctcct gaacggcgag gatgtaacgc tgccataacg
     3901 gatttaaagt cgaatcaaac atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg
     3961 ccaggaacgg agaaacacaa agcaacaaac cagtgacgat aatcaggtac agagacatcc
     4021 ctttgtattt gtgcaatgcc ggtactttgt aaaccagcca cgccgggatc aggcacccta
     4081 ccatgccgaa aatcgggcta cagatagagg tgaagctcaa caccggtgcg ttcagtacga
     4141 tggcgctcca ggccagcaaa atggcgaaaa tcatgatgcc gcgctgaaca agattttcgt
     4201 taatcttctc ggcaggcatc ttgcgacgca ggatgttcat tacgatccct tgcgttgctt
     4261 cgcgaaagcc taaatagacg ccaaagaatg cagtcatgac agcaaagata ttgagaatga
     4321 cgctgaccac tttcacccac gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca
     4381 gcgcggaaat attctgctca taggctttta ccgcttcgtc atgtcccatc gccagcgtga
     4441 acgacacggc gtagaaaaag acggtgacaa acaaaatgcc aaacgcgata ttcattgccc
     4501 gcaatgcttt atgccgcgcc acttcaattg atttttcccg cgagcgataa gagatcacca
     4561 tcgggcttaa cgtctggata aacagaatcg acgtcagggt aaacggcagc gtaataatgg
     4621 cgtttttcac cagcagcccc agcggcggta gcgaaccgac gttatacaga tgccacattc
     4681 ccaccatcga cacgcccagc gccgcgacca ccagcagctt ggtcagcacc atgccggtcg
     4741 aaattttgaa taacaatttc tcgccgcgtg aagagatcgc caccagaatg caaatcagta
     4801 ccagaccata aaagggactg tctgacagca acccttccgt cacgccgaag gtatgcagat
     4861 aggaagcact atcgttggtg atggcggtgg aatagacgaa catccaaatc accagcatta
     4921 cgaaatagag cgcacctaac aggatgcccc agtttttacc taaataaccg ctaatgacac
     4981 tcgggtaatc tttacattct ggtgattctg ccagcgtatt aataaacaac cgctgaaaca
     5041 gatacattgc cgggtaacca atcaccgatg agagcaaaaa tacccacaat cccatcaaac
     5101 cgacctgcac cgggagaaaa acaatccccg cgccaatcgc catcccgata ctcataatca
     5161 cccagccggt gtcagtactg tcgaatttga tcgcttctcg ccactcgctc tcactcattc
     5221 cagcacgacc cgccggggtc gaagcgtctg caatgacgcc tttattcgat gcaatttcca
     5281 taatttctcg ctcaatattt tgtagggctt attatttttt tccgagccgc atcaaggcga
     5341 tatgcggtct acgtttttgc aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc
     5401 agagaaaatc ttcacaatca agactttgaa ataacggaga tggataagaa ttttctacta
     5461 aattaatcgc aagagaatat tcattctcta tttgcggcag atcacaaaaa aaaggtgcac
     5521 atttgtgcac ccaaggatga aagctgacag caatgtcagc cgcagaccac tttaatggcc
     5581 agtcctccgc gtgatgtttc gcggtattta tcgttcatat ctttgccggt ttcatacatc
     5641 gtctcgatca ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc
     5701 gcggcgttta ctgctttcac ggcattaatg gcattacgtt caatgcacgg gatttgtacc
     5761 tgtccggcaa ccggatcgca ggtcagccca aggttatgct ccatcgcgat ttccgccgca
     5821 ttgcatacct gcgccggact gccgcccagt agttcagtta accctgccgc cgccattgaa
     5881 caggccacgc caatctcccc ctgacagccg acctccgcgc cagagatgga ggcgttcatt
     5941 ttatacagcg cgccaatagc ccccgctgcc agaaaatagc gggcaattga ccgctcgttt
     6001 accggacgac ggaacttatc gtaataagcc agcactgccg gaataatgcc gcacgcaccg
     6061 ttagtcggtg ccgttaccac gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg
     6121 tacatgttga tccagtcgat gacattcatc ggatcgttag agatgttatc gctggaaacc
     6181 agctgacgac gcagcgctac ggcacgacgc ggcacattga gcggaccagg cagcacgcct
     6241 tcagtgttca tcccgcgttc aataccgtca tgcatcactt gccagatacg ggcaaaccct
     6301 gcgtcaattt ccgctttgct gcgcagcgcc agctcgttgt gcatcatcag gccagatata
     6361 gacaggccgt tgtaatcaca cattttcagc agttcacctg ctgagtggaa atcgtaaggt
     6421 acggacgttt cgacatcgtg cgacaggccg aagtgttctt cttcgacaat aaacccgccg
     6481 ccgacagagt aataggtttt acttaatagc gcttcctgtc ccttccaggc agtgatccgc
     6541 attccgttct catggcgagg caacatttcg ggatggaaga taatgttctt tgctacagga
     6601 aaatcaacaa tatgcgcacc agatgccact ggcagccgcc cgctgcgcgt tactaactct
     6661 ataaatgcag ggatctcatc aatgacaaca tcctgcggac tgtttcctgc cagtcccatg
     6721 ataatggcga catccgtggc atggcctttg cccgtcagtg acaacgaccc atacagatcg
     6781 accacaatat ggctcgtcgc ggttaataag ccgctacttt ccagccgatc aataaaactt
     6841 tttccggcat tcattggccc cacggtatgc gaactggagg gaccaatccc aattttgaaa
     6901 atatcgaatg cactaatcat atccacaccc tcggattgcc gttcagtgaa gtggagcgga
     6961 acgaccttgc gaccgtcccg ctcacgaggc tttacgcact acgtactgcg atagcttcaa
     7021 tttccagctt cacatctttc ggcaaacgcg caacctgcac acagctccgg gtcgggtagg
     7081 tcgcctgatg ctcatcgaag aactgcttat agacttcatt gatggtggca aaatcattca
     7141 gatcggtgat gaacacggtc atcttgatga tatcgcccac gctcagcccg gcagcaacca
     7201 cgatcgcttt gacgttttcg aggcttaaac gcgcctgatc ttgcacatca gccgggatct
     7261 caccggtctg cgggcaaacc ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa
     7321 cgccctgaac ataagggccg attgcgcctg gggcacgttg cgtttcgata atctttttca
     7381 tacatcctcc ggcgtcagag cgcctgggta aaagtacgtg aaataacatc ctgttgctgt
     7441 tcacgggtca gtgcgttgaa gcgcacggcg tagccagaga cacggattgt caggttagga
     7501 tatttttccg ggtgctcgat ggcatccagc agcatttccc gattcattac gttgacgttg
     7561 aggtgttgac cgccttcgac atccgcttcg tggtggaaat acccatccag caggccgaca
     7621 aggttggttt tacgtactgg atcttctttg cccagcgccg caggaacgat tgagaaggtg
     7681 tacgagatcc catctttggc gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc
     7741 acggcacctt tgcggtcacg accatgcatc gggttagcgc ccggcgcgaa cggtgttccg
     7801 gcgcgacgac cgtccggcgt attaccggtt ttctggccgt acaccacgtt agaagtgata
     7861 gtcagaatcg actgggtagt gacggcgttg cgataggttg gcagcgcttt aattttcttc
     7921 ataaagcgtt caaccaggtc gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac
     7981 tgcggatatt caccgtcgat ttcaaagtcc accgccaggc cgttttcgtc acggattggt
     8041 ttcacgcggg catatttgat ggcagacagg gagtccgtcg ccaccgacag gcccgcgatg
     8101 ccgcatgcca tagtgcgata gacatcacga tcgtgcagcg ccatcagcga agcttcgtag
     8161 ctgtacttgt cgtgcatgta gtgaatgata ttcagcgcgc tgatgtactg caccgccagc
     8221 cagtccatga agtgatcgag gctgtccatc actttgtcgt aatccagcac gtcgtccatc
     8281 agcggtgctg ttttcggccc gacctgaatc ttcagcttct cgtccacccc gccgttaatt
     8341 gcgtagagca gcgttttcgc caggttagcg cgtgcaccaa agaactgcat ttgcttacca
     8401 atcaccattg ggctgacgca gcaggcaatc gcgtaatcgt cgctgttgaa gtcagtacgc
     8461 atcagatcgt cattttcata ctgcaaggaa gaggtgacga tcgacacctg cgcggcatat
     8521 tttttgaagg caatcggtaa ttcttccgac caaagaatgg tcaggttagg ttccggtgcc
     8581 ggccccatag tgtgcagggt gtgcaaatag cggaaggagt ttttggtcac cagcgtacga
     8641 ccgtccagcc ccatcccgcc gatcacttcc gtcgcccaga ttgggtcgcc ggagaacagc
     8701 gaatcaaatt ccggtgtacg caggaagcgt accatacgga tcttcatgat gaagtgatcg
     8761 atcagttcct gtgcctgctg ctcattgagt acgccagctt taaagtcgcg ctcaatgtag
     8821 atatcgagga acgatgccgt gcggcccagc gacatcgcgc cgccattttg cgatttcact
     8881 gccgccagat aagcgaagta gagccactgc accgcttcct gcgcattctg cgccgggcga
     8941 gagatatcaa agccatattt cgccgccatt tcctgaatct gcaacagcgc atgacgatgc
     9001 tctgccagct cctcacgcag acggatggtg gcttccagat cctcgccttt ttccagacga
     9061 gactggagat cggcaaattg cagttcgcgt tcacgtacca gataactgat gccatacagc
     9121 gctacgcggc gatagtcacc gataatgcgc ccacggccat agccatctgg taaaccggtc
     9181 agcacgccag atttacggca gcgcagcata tccggtgagt aaacatcaaa tacgccctgg
     9241 ttatgggttt tacgcagatc ggtaaacaga tattcaaatt cactgtccat ttcacggcca
     9301 taggcgtgga atgaactttt aatcatatta atgccaccga acgggtgtag cgcacgtttc
     9361 aacggcgcat ccgtttgcag gccaacaatt ttttccagcg gctggttaat atatcccgca
     9421 tcatgagcgg taattgtggt ggcaatattg gtatcgaaat caaccggcgc gtgggttgcg
     9481 ttttcgatac ggataccttc cattactttt tcccacaatt ccgtggtggc aggcgtcgct
     9541 tcggcgagga aagattcatc gccttcatac ggtgtatagt tatgttgaat aaaatcgcgg
     9601 acattaattt cgtttttcca gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc
     9661 ttatcgctgg tatcaatatc taccttcatg aaaaataatc tctctacaat acttcaacta
     9721 aattatgcaa attctgcggg cgcgttaact ttgcctaaat gaatggcatc caaagcaatc
     9781 attttttctt cgttagtcgg aataacagcg caaatgacac gcgcattttc actggaaaca
     9841 attcgctcac cacaggagtt agagcgatta ttcatttctg tatcaatctc tacgcctaat
     9901 acagccaaat gttccatgac cagacgacga attaagcttg aattctctcc tattccgccg
     9961 gtgaatataa ttccatccag gcgacgtaat gaagctgcgt gtccggcaat atgacgggca
    10021 attcggtgaa caaaggtttt aattgccagt tgcgcgcgtt cgtgaccttc atgccaggct
    10081 ttttccagaa cacgtaaatc cgaagaaaga ccggaaatac ctaataatcc cgactcttta
    10141 ttcactacgc gttccaggtc acccaggctc tggttggttt ggctggcgac ccaggacatc
    10201 gcaccaaagt cgacatcgcc actgcgggta cccatcatca agccttccag cggcgtcatt
    10261 cccattgaag tatcaacact ctgaccgttg cgaaccgcgc agattgacgc gccattgcca
    10321 agatgcgcca caaccaggcc ggagtcatct tccgccagat tcagcagcga atgtgcgcgc
    10381 tgggaaacat agcggtgcga cgtgccgtgg aaaccataac ggcgtacacc taactcttca
    10441 taatatttcc acggcaggcc gtataaataa gcttccggag ccatcgtctg gtggaaactg
    10501 gtatcaaata ccgccacctg agttacgccc ggaaataatt gctgcgccga ttcaatacca
    10561 cttaaattgg cgtaattatg caggggtgcc agtggagaaa cgcgacggat attatcaatg
    10621 acttcatcgg taataatggc ggactcggta aaaatactgc cgccgtgagc gatgcggtgg
    10681 ccaattaagg ccacactgtc atttaaattc cgtttttcca gttcaaatgc aattgccttc
    10741 aatgcacctt cgtagctgtg gtgagccagc ggtgctggct ctcccccatt tacggataag
    10801 aatgcatttt ccgagttaat accgtcggca atacctgaca ttaatacttc acagtcgctg
    10861 gcatcgagta cggaaaactt aatcgaagac gaaccacagt taataaccaa aacaaccgga
    10921 aattcattca tctcttttct catcctgagt tacggattaa aacagtttgt atacgatgtt
    10981 caggatggtc agcagaccaa tcacggtaac aaacacgtta tccagacgac cacggtattt
    11041 cgccagagac ggcgctttac ggatggcata catcggcaac aggcacagca gggatgcgat
    11101 aatcggtgcg cccatggctt caataaggtc gaggatgttc gggttggcgt aggcaacaac
    11161 ccaggtggag cccatgatga agatcatgct gatagtgttc agtttaccca gcgacacttt
    11221 agttttgtcg cctttataac cgaacttcag aatcagacca ttcaagcctt ccagcgtccc
    11281 cagatagtga ccgaagaaag atttgaagat agccacgagt gcgatgatgg aagccgcata
    11341 ttccagtgta atcgcgaacg ttgttttggt accggtcatg gacgcaaagt ggttagccag
    11401 ataagaaagc actggaatat tctgcgcttt ggcttccgcc atgttggccg gagacagcgt
    11461 aaacaggcag ctaaaggcaa agaacatcac cactgcaacc atcagcatgc tggcacgaga
    11521 aatgatttgg gaacatttac gttcggtgaa gtcgcgaccg aagtctttct catactcttc
    11581 acgtttagaa accacgaagg aagagacgat tggcgagaag ttaaaggaga aaaccatgat
    11641 ggaaatcccc agccagacag tgatcaggat accgtcatga ccggttaacg acagcgaacc
    11701 gaggtcaacc tggtcgataa ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag
    11761 caccaggctg gcgataaacg gccataccag gtagctcatc actttaacca tcagatcctt
    11821 accaaaccag atgacgaaag ccatcagcag caacaggaac agcgccacaa agccgcgatt
    11881 cagcggtgca aatccgagct ggttttccca gaacgtcata aaggtattgg taatagtaac
    11941 gccataaatc cacagcagtg ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc
    12001 gccagtttta ccaaaatgct cttccaccgt ttccgtaatg ttgccggaag ggttagagcc
    12061 agaaagacac agacgcgcca gcgcccggtg gcaataaaat gcgatggggt atgccaatac
    12121 caacatcaga agaatcggga tcagtccgcc aaaacctgcg cggataggga agaacagcac
    12181 cccggcgccg attgccgtac caaacaagcc taacgtccat gtggtatctg atttacgcca
    12241 ggacgattgt tttgtctggc tggatacaat gctatctgaa gtactcatat cctatcctca
    12301 acgaattaat taagcgtcaa cgaaaccggt gatttgagag acgcgagaaa gatcgatatt
    12361 gccgccggaa ataatactga cggtttttct gttttgaata tattggtcta atttaccgct
    12421 taataatgca gcacatgcca gagcgcctgc gccttcggtg acgactttat tgcgctgaat
    12481 taaggcaatc atactgtttc tgatttcgtc ttcgctgacc agcacgatgt catcgactaa
    12541 ttcacgaacg atttcgtaag ttaaattacc cgggcgggag acatcacaac catccgccag
    12601 ggtgccggta gttcggtgcg tggttatttc tccggagtgg aaagaagccg ccatgccgtg
    12661 aacgttttca gactgtacgc caataacacg aatggtcggg ttaatagatt taattgccac
    12721 cgcaatacca gcaattaaac cgccaccacc aattggcaca atcacgttat cgacatcata
    12781 gagatcttcc ataatttcca gaccaatcgt tccctggcca gcaatcactt tcggatcatc
    12841 gtaaggtggg ataaaaatac ggccttccat ttcgacaatt tcgctcactt tagcgatagt
    12901 gtcgttgaaa ttatcaccat gcagaacgac ttctgcggag tagtcgcacg ttgccgctac
    12961 tttggatttt ggcgcacctt ttggcatcac cactttaccg tcgataccca gcatcgcgca
    13021 ggagagcgaa accccttgcg catggttgcc cgcagaacag gccactacac ctttacgttt
    13081 ttccgcatca gtcagtgaac ttaatttatt aaatgcgcca cgaattttaa atgaacccgt
    13141 acgctgcata ttttcaaact tcaggaatat ttcacctttg caacgttcac taaaatagtt
    13201 tgagcgaggc atgcctgttt tataaattcg cccagccagt cgttgtttcg cttcaataat
    13261 gtcatcaata gcaaccggca gatcgtatgt aatatgcatt ataaaacctc ttcgcctgaa
    13321 ttaaatatgt aggtaaccgt aaccgacacc tgcaaaacag acaggtggat tatttatgtt
    13381 taaataaaaa caaataacta accaacttct attaattgcc ttcgtctaca cccattataa
    13441 gatgaatact ctttggctaa ttccaccaaa accgatgctg cttttttaat acgataattt
    13501 ttcgaccata ccacggcata ttgtgccaca ggtaatgttt cttcaaccgg aatagtaata
    13561 aattgatttg aaccaaaagg tgacgtcata tcacaaggaa ttacagttaa gaaatcagca
    13621 ttgagaacaa gattataaat tgtcacgact gagtcggttt taacgatgtt ttcaatactg
    13681 atgccatttc tttgtaacgt agtaagaagc tcgctgtagt accccatatt ggtttgtggc
    13741 aacacccact gttcgttctt caacgactcc agcgtggtgg tgccggtgca tgttcgggac
    13801 ttactggcta ccagcacaaa ctcggactcg aacagcggct caacatgtaa atcctgaagc
    13861 ttcatttctg cacttaacgt accaatcgca aaatccagtc gaccgtcgcg gattgccggt
    13921 aagaacgaag acagttgcgc ttcatacata gaaacctgcg ctttcgggaa cacctctttg
    13981 aacttgttga tcatccctga cataaaagta aaaccaatca atgaaggaaa accaaatgag
    14041 acttccacca ccgcctcaga agacataccg cttatctcat taaccatatt tttcatttca
    14101 cgggtaatgg attcggaacg ggagagtaac aattgaccgg caggtgttaa tgttacaccg
    14161 gtatttttcc gcaccactaa ttccacacca aaataatctt caatatcgtt aatgatttta
    14221 ctgacggccg gttgagttaa ccctaattct tttgcagccg agccgataga gccacttcta
    14281 atgacttcct gaaagactac caggtgctgc gttttcggaa gaagaatagt gctcataacg
    14341 acctacgtta attacctcat tgacggcatg aagtgtatca aaatgaaatg aacaggatat
    14401 gtgcgaccac tcacaaatta actttcaata ctttccatgg tatcgttacg caaattaaat
    14461 acctttaaat atcaacaagt
//
LOCUS       DAZFJF010000108        14393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFJF010000108 DAZFJF010000000
VERSION     DAZFJF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.108"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            80..646
                     /locus_tag="ACHKAU_003732"
     CDS             80..646
                     /locus_tag="ACHKAU_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5532716.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            697..3156
                     /gene="ybgQ"
                     /locus_tag="ACHKAU_003733"
     CDS             697..3156
                     /gene="ybgQ"
                     /locus_tag="ACHKAU_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5532717.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHARLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3171..3902
                     /gene="ybgP"
                     /locus_tag="ACHKAU_003734"
     CDS             3171..3902
                     /gene="ybgP"
                     /locus_tag="ACHKAU_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5532718.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3899..4960
                     /gene="ybgO"
                     /locus_tag="ACHKAU_003735"
     CDS             3899..4960
                     /gene="ybgO"
                     /locus_tag="ACHKAU_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5532719.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5112..6158
                     /gene="abrB"
                     /locus_tag="ACHKAU_003736"
     CDS             5112..6158
                     /gene="abrB"
                     /locus_tag="ACHKAU_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5532720.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6155..6946)
                     /gene="nei"
                     /locus_tag="ACHKAU_003737"
     CDS             complement(6155..6946)
                     /gene="nei"
                     /locus_tag="ACHKAU_003737"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO5532721.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6982..7716)
                     /gene="pxpA"
                     /locus_tag="ACHKAU_003738"
     CDS             complement(6982..7716)
                     /gene="pxpA"
                     /locus_tag="ACHKAU_003738"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO5532722.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7706..8638)
                     /gene="pxpC"
                     /locus_tag="ACHKAU_003739"
     CDS             complement(7706..8638)
                     /gene="pxpC"
                     /locus_tag="ACHKAU_003739"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO5532723.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8632..9288)
                     /gene="pxpB"
                     /locus_tag="ACHKAU_003740"
     CDS             complement(8632..9288)
                     /gene="pxpB"
                     /locus_tag="ACHKAU_003740"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO5532724.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9311..10054)
                     /gene="ybgI"
                     /locus_tag="ACHKAU_003741"
     CDS             complement(9311..10054)
                     /gene="ybgI"
                     /locus_tag="ACHKAU_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO5532725.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10325..11806
                     /gene="dtpD"
                     /locus_tag="ACHKAU_003742"
     CDS             10325..11806
                     /gene="dtpD"
                     /locus_tag="ACHKAU_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO5532726.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11848..13266)
                     /gene="phrB"
                     /locus_tag="ACHKAU_003743"
     CDS             complement(11848..13266)
                     /gene="phrB"
                     /locus_tag="ACHKAU_003743"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO5532727.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13263..13772)
                     /gene="ybgA"
                     /locus_tag="ACHKAU_003744"
     CDS             complement(13263..13772)
                     /gene="ybgA"
                     /locus_tag="ACHKAU_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO5532728.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13923..14393)
                     /locus_tag="ACHKAU_003745"
                     /pseudo
     CDS             complement(13923..14393)
                     /locus_tag="ACHKAU_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3465 a         3848 c         3493 g         3587 t
ORIGIN      
        1 aaaactaaaa acattcgaca atcaaataag atattcttgc accgatatac ttttttcttt
       61 tctgttaaag gactccgtta tgtttaaagg acaaaaaaca ttggccgcac tggccgtatc
      121 tctgctgttc acagcacctg tttatgcggc tgatgaagga tccggtgaaa ttcactttaa
      181 aggtgaagtt attgaagcac cgtgtgaaat acatcaggat gatattgata aagaggttga
      241 actcggtcag gtgaccacca gccacattaa tcagtcacat cacagcgatg ccgttgctgt
      301 cgacctgcgc ttagtcaact gtgatctgga aaactccagc aacggttccg gtggcaagat
      361 ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc accggcgcag atccgattct
      421 caacaacacc agcacgggtg aagccaccgg cgtcggcgta cgtttaatga ataaagatca
      481 aagcaatatc gttttaggca ccgctactcc agatatcgac ctggctccga cctccagcga
      541 acaaacgctg aatttctttg cctggatgga acaaattgat caggctacac ctgtaacgcc
      601 aggcgcagtt acggcgaatg caacctacgt gctggattat aaataagtat tactttggcg
      661 gcgctatcgc cgcctttcat aaaatattta tcaggtatgg acacagtgaa tatttatcga
      721 ctctcttttg tatcctgcct ggtcgtggcg atgccttgcg cattggcggt cgaattcaac
      781 cttaatgttc tcgataaatc aatgcgcgac cgcattgata tttcattatt aaaggaaaaa
      841 ggagtcattg ctcccggtga gtattttgtt agcgttgcgg taaataataa ccaaatcagt
      901 aacgggcaaa agattaactg gcacaaaaat gacgataaaa ccattccgtg catcaatgat
      961 ttactggtcg ataaatttgg cttaaaacct gaagtccgtc agtcgttacc attgataaat
     1021 cagtgcgtcg attttagctc ccgacctgaa atgctcttca atttcgatca agccaatcag
     1081 caactaaata tcaccattcc gcaagcctgg ctggcgtggc actcagaaaa ctggacccca
     1141 ccctccacat ggaaagaagg tgtcgccggt gtcctgatgg attacaactt gtttgccagc
     1201 agctaccgcc cacaggatgg cagcagcagc actaatctta acgcctatgg aacagccgga
     1261 atcaacacgg gggcatggcg cttacgcagt gattatcagt tgaatcagac tgatagcgat
     1321 gataaccatg aacagtcagg cgaaatatca cgtacctatc tttttcgccc gttaccgcaa
     1381 ttaggttcaa aattaaccct cggcgaaacg gattttagtt ccaatatttt cgacggtttt
     1441 tcttataccg gcgcggcact ggcaagtgac gatcgaatgt tgccatggga actacgcggc
     1501 tacgccccac aaattagcgg tattgcacag accaatgcca cggtgacgat cagtcaatca
     1561 ggccgcgtca tttaccagaa aaaagtccca ccaggcccat ttatcattga cgaccttaat
     1621 cagtctgttc agggcacact ggatgtcaaa gtgacggaag aagatggtcg ggtgaacaat
     1681 ttccaggttt cggcagcatc gacgcccttc ctgactcgtc agggacaggt tcgctataaa
     1741 ctggccgcgg gtcagccacg gccctccatg tcacatcaaa ctgaaaatga aacctttttt
     1801 agcaatgatg tttcctgggg gatgctgtca aacacctcgc tgtacggcgg cctgctgctt
     1861 tctggtgatg actaccattc tgccgcaata ggtatcgggc aaaatatgct gtggcttggc
     1921 gcgctgtcgt ttgatgtcac gtgggccagt agtcactttg atactcagca ggacgagcgt
     1981 ggcttaagct accgttttaa ttacagcaaa caagtggatg ccaccaacag cacgatttcc
     2041 ctcgccgctt atcgtttctc cgatcgtcat tttcacagct acgccaacta tcttgatcac
     2101 aaatacaacg acagcgatgc gcaggacgaa aaacagacga tcagcttatc tgtgggccaa
     2161 ccgattaccc cactaaacct caatctttac gccaacctgc tacatcaaac ctggtggaat
     2221 gcagacgcct ccacgaccgc caacatcacg gcaggtttta atgttgatat tggtgactgg
     2281 agagatatct ctatttcgac gtcattcaat acaacccatt acgaagataa agatcgcgac
     2341 aaccagattt acctgtcgat ttcgctcccc ttcggtaacg gtggtcgggt tggctatgac
     2401 atgcaaaaca gtagccacag caccacacac cgcatgtcgt ggaacgatac gctggatgaa
     2461 cgtaatagct ggggcatgtc tgccggactg caatccgacc gtccggacaa tggagcccag
     2521 gtgagcggta actatcagca tctgagttca gcgggtgagt gggatatttc tggtacctat
     2581 gccgccaatg attacagttc cgtcagcagc agctggagcg gttctttcac cgcaacccaa
     2641 tatggtgcag cgtttcatcg ccgcagctcc accaatgaac cacgcctgat ggtcagcacc
     2701 gatggcgtgg cagatattcc ggttcagggc aatctcgact acaccaacca ttttggcatt
     2761 gcggtggtgc cgttgatttc cagttaccag ccttccaccg tggcggtgaa catgaatgac
     2821 ttacccgacg gcgtaacagt tgcagaaaac gttatcaaag aaacgtggat tgaaggcgcg
     2881 ataggttaca aatcactggc ttcccgttcc ggtaaagacg ttaacgtcat cattcgcaac
     2941 gccagcggtc agtttcctcc cctcggagcg gatatccgcc aggatgacag cggcattagc
     3001 gtggggatgg ttggcgagga aggacatgcc aggttaagcg gagtcgctga aaatcaaaag
     3061 tttaccgtgg tctggggtga tagccagcat tgctcgctcc atcttcctga acatatggaa
     3121 gacaccgcaa atcgcctgat tttaccttgt cattaattaa ggaaatgaaa atgacattta
     3181 tgaaaggact accccttttg ttgttggttg ccagtctgtg cagccacgct gcactacaac
     3241 ccgatcgcac tcgtattgtg tttaacgcta atgataaagc taccagtctg cgggtggata
     3301 atcgtagcga taaactcccc tacctcgctt attcctggct cgaaaatgaa aaaggggaga
     3361 aaagcgatga tcttctggtc gccctgccgc cgatacaacg tctggaaccg aaagcaacaa
     3421 cgcaggtacg gatagtaaag caagcctcga ccacaaaatt accgggcgat cgcgaaacgc
     3481 tatttttcta caatatgcgt gaaattcctc ccgccccgga aaaaaacagc gaccatgccg
     3541 ttctgcaagt cgccattcag agtcgtatca aggtgttctg gcgaccggct gcattacgca
     3601 agaaagcagg agaaaaggtc gaactgcagt tacaggtcag ccagcagggc aaccaactga
     3661 cactgaaaaa tcctaccgcg tattatctga ccattgccta cctgggacga aatgaaaaag
     3721 gcgttctccc cggcttcaaa accgtgatgg ttgcgccttt tagtacggtc aacacaaaca
     3781 tcggaaatta tagcggcagc cagttttatc tcggttatat ggatgattac ggcgcgttac
     3841 gcatgacgac gctcaactac agcggacaat gccatttaca agccgtggag gcgaagaaat
     3901 gagtgctggc aagggagtgt tgctcgtcat ctgtctgtta tttctgccgt tgaagtctgc
     3961 gcttgccctg aactgctact ttggttcatc cggtggctcg gtagaaaaat cagaagcgat
     4021 tcaacctttt gctgtaccag gcaatgccaa actcggcgat aagatttggg aatctgacga
     4081 tattaaaatt cccgtctatt gtgacaacaa taccaacggc aattttgaaa gtgagcatgt
     4141 ttatgcctgg gtaaacccct acccgggggt gcaggatcgc tattatcaac tcggcgtaac
     4201 ttacaatggc gttgattatg acgccaacca gggaaaaagc cgcatcgaca ctaaccagtg
     4261 tattgacagt aagaacattg acatttatac ccctgagcag attatcgcga tgggatggca
     4321 gaacaaaatt tgctcgggtg atcccgctaa tattcacatg tcacgtacct ttctcgctcg
     4381 catgcggtta tacgtcaaaa tacgagaaat gccgccgcat gattatcaaa gtacgcttag
     4441 cgactatatc gtcgtgcaat ttgacggtgc cggtagcgtt aacgaagatc ctactgccca
     4501 aaacctgaaa tatcatatta ccggtctgga aaacattcgc gtgctggatt gcagcgtcaa
     4561 tttttccatt tcaccggaaa cacaggtgat tgattttggt aaatttaatt tgctggatat
     4621 tcgtcgccac acaatgtcga aaacattcag tattaagacg acaaaatcac aaaatgacca
     4681 atgcaccgac ggatttaaag tcagttcctc cttttatacc gaagaaacgt tggttgaaga
     4741 agataaagca ttgttgattg gtaatggtct gaagctacgt ttgctggatg aaaacgcctc
     4801 gccttatacc ttcaataaat atgctgaata cgccgatttc accagcgaca tgttggtcta
     4861 cgaaaaaacc tatacggctg aactttcgtc cattgcaggt acccccatcg aggctgggcc
     4921 cttcgataca gtggtgcttt ttaaaattaa ctataactga tttgccatga tgccctttgc
     4981 agccggaaag ggcatctctt tcagaaaatt gctgattttc cccttttcct tgattgctgt
     5041 tattttttcc tggcccggct ttctttatgc tgtatcgata caccaaagag aaaataatga
     5101 gggagcgaag gatgccagtt ttgcagtggg gaatgttatg tgtgctatca cttctccttt
     5161 ctattggttt cctcgcggta catctcccgg cggcgctatt acttgggccg atgatcgccg
     5221 ggatcatctt cagtatgcgc ggaattactc tgcaacttcc ccgctccgct tttctcgccg
     5281 cgcaggccat tcttggctgc atgattgcgc aaaacctcac tggttcgatt ctcaccacgc
     5341 tggccgccaa ctggccgatc gtgcttgcta ttttactggt gacgctgctt tccagcgcca
     5401 tcgtgggttg gttattggtg cgctatagct cactgcctgg aaataccggt gcctggggtt
     5461 cttctccagg cggcgcagcg gcaatggtcg ctatggcgca agattacggc gcagatattc
     5521 gcctggtggc gtttatgcaa tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg
     5581 tcacccgcat gatgctgggt gataacgctg aagcggttaa ccagcaaatc gtctggttcc
     5641 cgccagtgag cattaatctc ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg
     5701 gatgcatgct gcgtcttcct tccggcacga tgctcatccc gatgctggcg ggcgcagtgc
     5761 tccagtctgg tcagctcatc accatcgaac tcccggaatg gctgcttgcg atggcgtata
     5821 tggcaattgg ctggcggatt ggtcttggtt tcgataaaca aatcttactg cgggcactac
     5881 gcccgctacc gcaaatcctg ctgtcgattt ttgctctgtt ggctatttgt gcgggtatgg
     5941 cgtgggggct gacccggttt atgcatattg attttatgac tgcctacctc gccaccagcc
     6001 ccggcgggct tgataccgtg gcggtcatcg ccgcagggag caatgccgat atggcgctta
     6061 ttatggcgat gcaaaccctg cgcctgttca gtattttgct gacgggacct gccattgcac
     6121 ggtttatttc aacctatgcg ccgaagcggt cggcttagtg ctggcagcca gggcaccagt
     6181 aaaacgggcg agatgacagc gtggttttct caatgatgct gccacaacgt tcgcacggtt
     6241 cgccatctcg atgaaaaacc ttaaagcgaa acagcgcccc atgatgctta ttctcatcca
     6301 cctgcccccg cgtagcgtaa gaaaatcgag gaatctccag taacgcgtgt gcgagtgcat
     6361 ccagttgcgc cgcattgaga tctttcgctt tatgatttcc agtcaaccca acctgccaga
     6421 ggatctccac ccgcaaataa ttgccaagtc ctgccagaaa cgcctgctcg agcagtaatc
     6481 ctgcaaactg acggttacga aagcgcggcg acaatacccg ttctttcacc acctccggcg
     6541 tcagattcgg atccagcaca tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt
     6601 gttccggggt caacatctca atatccgagg cgctataaag cagaatggtt ttgtcagccg
     6661 tttgcagttt tacccgcaat actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc
     6721 gccagacgcc gtaaagctga ttatggctgt agagcgttaa ttcgttggaa aaatgagtta
     6781 acaacgcctt accacgcgtt tccacatggg taacgtgttg accgataagt tgtgattgat
     6841 aagtttttaa ctgcgggaag gcaaaccaga catcagttag tggtttgcct ttgatcgccg
     6901 cctccaggtt atccgctgca cggcggatct ccgggccttc aggcatggtg atatcctttt
     6961 ttcattcttt attaagcggg gttatgctgc gacaacaatc ccctttttgg caaatgtagc
     7021 tcgtaatcgg cgggcgaacg ccagagcgtg ctcgccgtcg ccatgcaggc agaccgtttg
     7081 cgccgcgacc gttgcccatt cgccggtgat acttttgact ctgccgtgtt gcaccatctc
     7141 cagcgtttgc gccagcgcct gttgttcgtt ttcaatcaac gcgcctggct gacttcgcgg
     7201 caccagcgag ccgtcagcct gataaccgcg atcggcaaac acttcctcgc gcgttgtcag
     7261 gccatattgc ttgcctgcac gaatcagctc gcttcctgcc agcccaacga gaatcaatgc
     7321 cggatcgcaa gcgtataccg ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc
     7381 ctggttgtac aacatgccgt gcggtttgac atgacgcatt acgccgcctt gcgcacgggc
     7441 aatcgttgcc agcgcgccaa tttgatacag cgtctgggcg taaacggttt ctggcggcaa
     7501 ctgcatggcg ctgcgaccaa aattttccct gtcgggaaaa ctcgggtgag cgccaattgc
     7561 aacaccattt tttattgctt cacgcacgca agcctgcatg gtttgcgcat cgcctgcatg
     7621 aaatccacag gcaatattgg cagaggaaac cagcgttaat agctctgcgt cgctggcgca
     7681 gccttcgccc agatcggcgt tcaggtcaat tttcattgtg cagccgccac gctaattgtt
     7741 caaaataacg ttgctgatct tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa
     7801 aatgaatcgg ctgaccgagc ggaatttgcg caagatggta catatcagcc tcaatgatgc
     7861 aggcaatacg cgggtaacca ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc
     7921 cgttatgtgg cacctgcacc acgcccggta acaaaccgtg agataacagt tcgcgatcgg
     7981 tggtgcgttt taaaatttgc ccctgtaagc gatagcccat gcggttactt tgcgagctaa
     8041 gctgccaggg cgaacgccag aatgcatcct gcgaggcgcg atcgaactca tgatattccg
     8101 gccccggcaa ggcgcgaatg cggttgcccc acagcagctg tttaacgccc tgcgcttcca
     8161 taaaatcacg cttcgctttg ccaatcggga gtcggtcacc atccttcagt aaacggcctt
     8221 ccagcccgcc actccccact ttgagatcgg tgctgcatga ccccattacc ggcggaacat
     8281 caataccacc cgcgaccgcc agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta
     8341 aacgctggcc tgctttcatc ggcaatcgcc agccggtcca gacggcatta tcatccagcc
     8401 gcgcttcgca accggcaccc gtcagagcaa accacccatc agtttcgaac tcaacagtta
     8461 actgaccgag cgtgatctcc agcgcggggg cattggcgtc attacccacc agtaggttag
     8521 caatgcgtaa cgcgggcata tccagtgcgc cgcagtggct gatacccgac tggcgaaaac
     8581 cgtgacgacc gccatcctgc acagtggtat acatgcccgc acgaataatc ttcagcatac
     8641 tccctccttc tgcggtacaa agcgcacgct gtctcccgga cgtaataaga tgggttcgtc
     8701 acgcgccgga tcaaacaggc tgagtgaggt atgaccaatc aactgccagc cacccggcgt
     8761 tgccagcgga taaacaccag tctgcggccc gccgatcccg acagaacctg ccggaacgag
     8821 taagcgcggt tcagcgcgcc gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg
     8881 gaagcccggt tgaaaaccta aaaaccagac cacgtattcc acggaggagt gcaattcaac
     8941 aacctgtttt tcgctcaacc cgcaatgcgc cgcgaccacc gccaaatccg gtccgcctgc
     9001 accaccgtaa accaccggaa tttcaataaa gcgagactca ggttccaggg cctcgctctc
     9061 ctcccaccag cgttgcaaac gctctatggc atccagcgcc agcgactcag gattacgcaa
     9121 aatcaccgta atattgttca tgccgggaat ggcttcaacc acattcggca tatccaccag
     9181 acgctgcgcc agtcgccaga tccgtttctg gctagccagc gtcaccggcg gttccagttc
     9241 cagcactacc gccgtttcac ctatcagata acaacgcgct cgttgcactt tcgatccctc
     9301 tgattattcg ttatgcagga ttaggaatat caataaaggt cacatcaaga tcggtatttt
     9361 cattcagcca ctcgctcaat gcgcgaatac caccacgttc agtggcatgg tgacctgcag
     9421 cataaaaatg caatccctgc tcgcgggctg aatgaatggt ctgttcagaa acttcgccag
     9481 taataaaagc atccacgcca aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg
     9541 tgcaccaggc gacgcgctgg accacctcag gtccggtatc gccacaccat aacggcttac
     9601 gtcccagacg cgcttcaatc caggaggcta gttccagtcc cggcacaggc atggtcagct
     9661 ctccccacgg caccaacggc tcaatttcgc ccatgaccgt gatccccagt aacgccgcca
     9721 gctgtgcgtt attgcccagc tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt
     9781 tgatatcatt cgccagcaac gtttttaaac ggttacgctt cattccgcga atgaccggag
     9841 actctccttt ccagaagtag ccatgatgca caatgaccgc atccgcgccc agacgcactg
     9901 cctcatcgag caaagcctgg ctggcggtga caccggtaac aattttttgc accgtctctt
     9961 tgccttccac ctgcaaacca ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt
    10021 cgttaatcag ttgttccagt tcggtgtttt tcatcttcgc gccttccgta ttctgatagc
    10081 tacaccgtta acatagcgtt acctcgcctg ttcgtcgata gccaatcttt tgacatatta
    10141 agaaacaaac ccgacgccaa aagacgcaaa ttgtttatat catcacgctt cagctaatgt
    10201 atatttatgc aatcagacag catattgcat aaataatcag atctgtatca aagttttaac
    10261 tttaaggaaa cacaagcacg ccgcgcaact attacgtgac gttgccgtca attgtggaat
    10321 tattatgaat aaacacgcat cacaaccgcg cgctatttac tatgtcgttg cgctgcaaat
    10381 ctgggaatat tttagctttt acggcatgcg tgccctgctg attctctatc tcaccaatca
    10441 actaaaatac aacgatactc acgcctacga gttatttagc gcctactgtt cgctggtgta
    10501 tgtcacgcca atcctcggtg gctttttggc ggataaagtt ctcggcaatc gcatggcggt
    10561 gatgctgggg gcgttgttga tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca
    10621 tccgtcattc ctctatctgt ccctggcgat tatcgtttgt ggctatggtc tgtttaaatc
    10681 caatgtcagt tgcctgctcg gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg
    10741 tttctcgctg atgtatgcag cgggtaacgt ggggtctatt atcgcaccta tcgcctgtgg
    10801 ttacgcccag gaagagtaca gctgggcgat gggctttggc ctggcggcgg tgggtatgat
    10861 cgcgggtctg gtcattttct tatgtggcaa tcgtcatttc acacataccc gcggcgttaa
    10921 taaaaaagta ctgcgtgcaa caaactttct cctgcctaac tggggatggc tgctggttct
    10981 gctggtggca acgcctgcgc tgattaccgt actgttctgg aaagagtggt cggtatacgc
    11041 cttaattgtc gcgactatta ttggcctggg tgtactggca aaaatttatc gcaaagcaga
    11101 aaaccagaaa cagcgtaagg agctggggct gattgtgacg ctcaccttct tcagtatgtt
    11161 gttctgggcc ttcgcacaac agggcggcag ctcgattagc ctttatatcg accgcttcgt
    11221 taaccgcgat atgtttggtt ataccgttcc gactgctatg ttccagtcga ttaatgcctt
    11281 cgcagttatg ctgtgcggtg tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa
    11341 tcgtaccgtg cgcatctggg gaaaatttgc tcttggcctt ggcctgatga gcgccggatt
    11401 ctgcattctg accttaagcg cccgctggtc cgcaatgtat ggtcactctt ctctgccact
    11461 gatggtatta ggcctggcgg tgatgggctt tgcggaactg tttatcgacc cggttgccat
    11521 gtcgcaaatt acgcgcattg aaattccggg cgtgaccggc gtattaaccg gcatctacat
    11581 gctgctttct ggcgcgatcg cgaactatct ggcgggcgtg attgccgatc agacatcgca
    11641 ggcttcgttt gatgcttccg gggcgatcaa ctactccatc aatgcatata ttgaagtatt
    11701 tgatcaaatt acctggggcg cactggcgtg tgtaggactg gtactgatga tttggctgta
    11761 tcaggcgctg aaattcagaa accgcgcgct ggcgctggag tcataatagg ccagcaccat
    11821 acctgataaa acgctcgctg cgttttatca ggctcctttc ctcgcttctt cgtacgctgc
    11881 cagcgttcgc agccttgcct ctttgtgatc aactatcggt tgcggataat ccagcatcac
    11941 acctgctttc tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag
    12001 ctccggtaac catcggcgga taaactcacc ttcgcgatca aatttctcgc cctgggttgt
    12061 cgggttgaaa atacgaaaat acggcgctgc atcagtaccc gtagacgccg cccactgcca
    12121 gccaccgtta ttggctgcca aatcaccatc aatcagctgc gacatgaaat atcgctcgcc
    12181 ttcgcgccag tcgatcaaca aatctttcac cagaaaactg gctgaaatca tccgtagccg
    12241 gttatgcatc cagccagtgc tgttaagctg acgcatggcg gcatcgacaa tcgggtatcc
    12301 cgttttgcct tcctgccagg cctgtaaatg tgcgggattg ctctgccact gcacacgatc
    12361 cgtccaggca ataaacggac aatgtttaca caacgagggg taatacgtca tcagatgacg
    12421 gtagaactcg cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc
    12481 ctgcggctgt tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc
    12541 caggctggcg gacaaacgac tggtgccttc cactgccgga aaatctcgtt gttgctcata
    12601 ttctccggca ccgttctggc aaaactggcg taattgcgca atcgccgctt tttcttccac
    12661 cgggaaatgc gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgaggg
    12721 ctctatcgat ccgctactac gaacttttgg cgcagcgacg cactccggca tcccttcccg
    12781 cagccgtttc agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattacc
    12841 ggtcttcacc gcgccaggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt
    12901 acgcagcgct ctttcaactt ccacatcccg cgcccgctca ttcacttcat actgatagtt
    12961 ataaaacagg tgggtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc
    13021 gacaaagtca tccacttcac ggaacaataa aggaatacct ttttccgcaa gcgctatttg
    13081 tagcccattc agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtcgc
    13141 ccactggcgt ggtgtagcga tatacaacgc cagcacgcgt gcagacgaat tgcggcaggc
    13201 ggcagccagt gcgagattat cgtgcagacg taaatcctgg cgaaaccaga ccagatgggt
    13261 agtcataaaa ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc
    13321 tgccctgaaa gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc
    13381 gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa gcgccgccaa ctgctggcgt
    13441 tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga
    13501 tcgcgcgggt tggcgggatg agaaagcaaa acaattacgc gttggtagta ctggttgtaa
    13561 aaatcatcca gattttgcca ctggtgaata tcagccacga acgggccaag tttgcggtac
    13621 tccggctgag aatgcgccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc
    13681 ccgcgcgtta agccatgttc tttcagtcga tgtaactcat gaagggcgaa gatacggatt
    13741 agggtgctgt catcaaatcg ttgctgattc attactgctc ctgaacggtt tacaggagta
    13801 agtgtagacg ttgagaaatg atatttgtga agttaatcct gttgtagcag gacaaattta
    13861 cgaaagcctg ctccccgcaa ggactgacgt caggtagttt ctaaccatag ctgccctttc
    13921 tattcaaacg tgaaatcaat aagctcatca gggacatcgc aaacaatatg aagacgggtt
    13981 tcttctctgc cgtgactctt ttcactaatt gcgtaactgt catgcttggg attatttaat
    14041 tctttcagcg gaaatttttc ctcgaaggct ttatttagcc gcccctggtt tccttttaca
    14101 gcgaataaat aatcacctcc ctgtttttgt atcttctctg caatatcttt ctggcaaccc
    14161 atcgcatcgg ttgtgatgat ttttccttta atatccagca tgttaagaag ttctgggata
    14221 gctgtgatct cattggattt cttatccgtc ttgatctgcc cgatgaccag actgtgcatt
    14281 gttgagaacg cactaatgac atgaatcgct cccctgcggc gactcttgtc ataagagtgc
    14341 cggagcgttt ttccatcaat tgcaatgacg tctttatcat ttgatgaatg gca
//
LOCUS       DAZFJF010000109        14288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFJF010000109 DAZFJF010000000
VERSION     DAZFJF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.109"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            184..1563
                     /gene="ygjG"
                     /locus_tag="ACHKAU_003746"
     CDS             184..1563
                     /gene="ygjG"
                     /locus_tag="ACHKAU_003746"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO5532729.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(1605..1937)
                     /locus_tag="ACHKAU_003747"
     CDS             complement(1605..1937)
                     /locus_tag="ACHKAU_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO5532730.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            2156..3139
                     /gene="ebgR"
                     /locus_tag="ACHKAU_003748"
     CDS             2156..3139
                     /gene="ebgR"
                     /locus_tag="ACHKAU_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO5532731.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            3323..6415
                     /gene="ebgA"
                     /locus_tag="ACHKAU_003749"
     CDS             3323..6415
                     /gene="ebgA"
                     /locus_tag="ACHKAU_003749"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO5532732.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            6412..6861
                     /locus_tag="ACHKAU_003750"
     CDS             6412..6861
                     /locus_tag="ACHKAU_003750"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO5532733.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            6924..8357
                     /locus_tag="ACHKAU_003751"
     CDS             6924..8357
                     /locus_tag="ACHKAU_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5532734.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            8491..9561
                     /gene="ygjJ"
                     /locus_tag="ACHKAU_003752"
     CDS             8491..9561
                     /gene="ygjJ"
                     /locus_tag="ACHKAU_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO5532735.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            9578..11929
                     /gene="ygjK"
                     /locus_tag="ACHKAU_003753"
                     /pseudo
     CDS             9578..11929
                     /gene="ygjK"
                     /locus_tag="ACHKAU_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            12255..14273
                     /gene="fadH"
                     /locus_tag="ACHKAU_003754"
     CDS             12255..14273
                     /gene="fadH"
                     /locus_tag="ACHKAU_003754"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO5532736.1"
                     /translation="MSYPLLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVASSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHHVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT         3455 a         3808 c         3767 g         3258 t
ORIGIN      
        1 tcaaatccat acccgacaaa aaccgtgcaa aataacaaca aatgttaaca gatagcatta
       61 aatattgcac aaatgataac cgaatttgtg tttatcccga ttttcgcgat cgcagccgga
      121 gtggcgcaat ccctgcaata cttaaatcgg tatcatgtga tacgcgagcc tccggagcat
      181 attttgaaca ggttaccttc gagcgcatcg gctttagcgt gcagcgccca cgccctgaat
      241 ctcattgaga agcgaacgct ggatcatgag gagatgaaag cacttaaccg agaggtgatt
      301 gaatacttca aagagcatgt caatccgggg tttttagagt atcgcaaatc tgttaccgcc
      361 ggcggggatt acggagccgt agagtggcaa gcgggaggtt taaatacgct tgtcgacacc
      421 cagggacagg agtttatcga ctgcctggga ggttttggaa ttttcaacgt ggggcaccgt
      481 aatccagttg tggtttccgc cgtacagaat caacttgcga aacaaccgct gcacagccag
      541 gagctgctcg atccgttacg ggcgatgttg gcgaaaaccc ttgctgcgct aacgcccggt
      601 aaactgaaat acagcttctt ctgtaatagc ggcaccgagt ccgttgaagc agcgctgaag
      661 ctggcgaaag cttaccagtc accgcgcggc aagtttactt ttattgccac cagcggcgcg
      721 ttccacggta aatcacttgg cgcgctgtcg gccacggcga aatcgacctt ccgcaaaccg
      781 tttatgccac tgctgccggg cttccgtcat gtgccgtttg gcaatatcga agccatgcgc
      841 acggctctta acgagtgcaa aaaaaccggt gatgatgtgg ctgcggtgat cctcgaaccg
      901 attcagggtg aaggtggcgt aattctgccg ccgccgggct atctcaccgc cgtacgtaag
      961 ctatgcgatg agttcggcgc actgatgatc ctcgatgaag tacaaacggg catggggcgc
     1021 acgggcaaga tgttcgcctg cgagcatgag aacgtacagc cggatatcct ctgccttgcc
     1081 aaagcgctcg gcggcggcgt gatgccgatt ggcgcgacca tcgccactga agaggtgttt
     1141 tcagttctgt tcgacaaccc attcctgcat accaccacct ttggcggcaa cccgctggcc
     1201 tgtgcggcgg cgctggcgac catcaatgtg ttgctggagc agaacttacc ggctcaggct
     1261 gagcaaaaag gcgatatgtt gctggacggt ttccgtcaac tggcgcggga atatcccgat
     1321 ctggtacagg aagcgcgtgg taaagggatg ttgatggcaa ttgagtttgt tgataacgaa
     1381 atcggctata actttgccag cgagatgttc cgccagcgcg tactggtggc cggaacgctc
     1441 aataacgcca aaacgatccg cattgaaccg ccactgacac tgaccattga acagtgtgaa
     1501 ctggtgatca aagcggcgcg taaggcgctg gcggccatgc gagtaagtgt cgaagaagcg
     1561 taatatcaaa tcggatggcg atacgacgtc gccatccgat ctttttatac gacgcgtacg
     1621 cccgcaggca tcatccgctc cggcgttaac aacacgcttt cactgccgtc atcggtttcc
     1681 gcacacaaca gcatgcattc cgacgtttca ccacgcattt tcgctttttg cagattgcat
     1741 aagaccacca ccgttttccc catcagctct tcttcgctgt agtacggcac caggctggtc
     1801 acggtttgca gtgttttttc ccccacatca acctgtacga tgtacagctt gtcggcgttt
     1861 tcatggcgtt tcacttccac aatctttccg acgcgcattt ccagacgtgc aaaatcagcg
     1921 taagccacgg tttccattgt ttcctccctt cgggtaaaat tttactaaac tatagcaaaa
     1981 gtttttctca atcctgtagg ctaaaaatgg agaatgcagg cgtgatcaca ttcgtgagcc
     2041 gctgtgttac cgttacagcg tcaaagaaac gcgctttatt tactgaaaac aggtgacccg
     2101 ataagcactt cctctacaat gggggcgcac atcagggaaa gtaaaaaagg taaacatggc
     2161 aacactaaaa gacatcgcaa tcgaagctgg cgtatccctg gcgacagtat ccagggtctt
     2221 aaatgacgat ccgacattga atgtgaaaga agagacgaaa catcgcattc tcgagatcgc
     2281 cgaaaagctg gagtacaaga ccagtagtgc ccgtaaactc cagacaggtg cagtcaacca
     2341 acaccatatt ctggctatct acagctacca gcaggagctg gagatcaacg atccttacta
     2401 tctggcgatc cgccacggca ttgaaaccca gtgcgaaaag ctgggcatcg agctcaccaa
     2461 ctgttatgaa cacagcggct taccagacat taaaaacgtc accggtattt taattgtcgg
     2521 caaacccacg cccgccctgc gcgccgctgc cagcgcgttg accgacaata tctgttttat
     2581 cgactttcac gaacccggca gcggttacga tgcggtggat atcgatctgg cacgcatcag
     2641 taaagaaatc atcgacttct atatcaacca gggcgttaat cgtattggtt ttattggcgg
     2701 tgaagatgag cctggcaagg cggatattcg tgaggtcgcc tttgcggaat atggccgact
     2761 gaaacaagtg gtacgcgaag aggatatctg gcgcggcggt ttttccagtt cgtcgggtta
     2821 tgaactggca aaacaaatgc tggcgcggga agactatccg aaggcactgt ttgttgcttc
     2881 cgattccatt gctatcggcg tactgcgggc aattcatgaa cgaggcctga acatcccaca
     2941 ggatatttcg cttatcagcg ttaacgatat ccccaccgcg cgatttacct ttccgccgct
     3001 ctccaccgtg cgcatccatt ccgagatgat gggaagtcag ggcgttaacc tggtgtatga
     3061 aaaagcccgc gatggtcgcg cgctgccgct gttagtcttc gttcccagca aattaaaact
     3121 gcgcggcacg acccgttaaa tccccttaca cactgtccgg caatcgtttt tgccggacag
     3181 tgctgccgtt tattttcgtg atccagttaa agtaaatgca tttacctgct actttttagt
     3241 aaaaatttta ctaaactccc cagcaattac acaaactacc atcaccatga atggttccga
     3301 tttctctcta ccgggaggcc ctatgaatcg ctgggaaaac attcagctca cccacgaaaa
     3361 ccgacttgcg ccgcgtgcgt actttttttc atatgattct gttgcgcaag cgcgtacctt
     3421 tgcccgcgaa accagcagcc tgtttctgcc cttaagcggt cagtggaatt tccacttttt
     3481 tgaccatccg ctgcaagtac cagaagcctt cacctctgag ttaatggctg actgggggca
     3541 tattaccgtc cccgccatgt ggcaaatgga aggtcacggc aaactgcaat ataccgacga
     3601 aggttttccg ttccccatcg atgtgccgtt tgtccccagc gataacccaa ccggtgccta
     3661 tcaacgtatt ttcaccctca gcgacggctg gcagggtaaa cagacgctga ttaaatttga
     3721 cggcgtcgaa acctattttg aagtctatgt taacggtcag tatgtgggtt tcagcaaggg
     3781 cagtcgcctg accgcagagt ttgacatcag cgcgatggtt aaaaccggcg acaacctgtt
     3841 gtgtgtgcgc gtgatgcagt gggcggactc tacctacgtg gaagaccagg atatgtggtg
     3901 gtcagcgggg atcttccgcg atgtttatct ggtcggaaaa cacctaacgc atattaacga
     3961 tttcactgtg cgtaccgact ttgacgaagc ctattgcgat gccacgcttt cctgcgaagt
     4021 ggtgctggaa aatctcgccg cctcccctgt cgtcacgacg ctggaatata ccttgtttga
     4081 tggcgaacgc gtggtgcaca gcagcgccat tgatcatttg gcaattgaaa aactgaccag
     4141 cgccagcttt gcttttactg tcgaacagcc gcagcaatgg tcagcagaat ccccttatct
     4201 ttaccatctg gtcatgacgc tgaaagacgc caacggcaac gttctggaag tggtgccaca
     4261 acgcgttggc ttccgtgata tcaaagtgcg cgacggtctg ttctggatca ataaccgtta
     4321 tgtgatgctg cacggcgtca accgtcacga caacgatcat cgcaaaggcc gcgccgttgg
     4381 aatggatcgc gtcgagaaag atctccagtt gatgaagcag cacaatatca actccgtgcg
     4441 taccgctcac tacccgaacg atccgcgttt ttacgaactg tgtgatatct acggcttgtt
     4501 tgtgatggcg gaaaccgacg tcgaatcgca cggctttgct aatgtcggcg atatcagccg
     4561 tattaccgac gatccgcagt gggaaaaagt ctacgtcgag cgcattgttc gccatattca
     4621 cgcgcagaaa aaccatccgt cgatcatcat ctggtcgctg ggcaatgaat ccggctatgg
     4681 ctgtaacatc cgcgcgatgt accacgcagc gaaggcgctg gatgacacgc gactggtgca
     4741 ttacgaagaa gatcgcgatg ctgaagtggt cgatattatt tccaccatgt acacccgcgt
     4801 gccgctgatg aatgagtttg gtgaataccc gcatccgaag ccgcgcatca tctgtgaata
     4861 tgctcatgcg atggggaacg gaccaggcgg gctgacggag taccagaacg tcttctataa
     4921 gcacgattgt attcagggac attatgtttg ggagtggtgc gaccacggga tccaggcgca
     4981 ggatgacaac ggcaacgtct ggtataaatt cggcggcgac tacggcgact atcccaacaa
     5041 ctataacttc tgtcttgatg gtttgatcta ttccgatcag acgccgggac cgggcctgaa
     5101 agagtacaaa caggttatcg cgccggtaaa aatccacgcg ctggatctga ctcgcggcga
     5161 gctgaaagtc gaaaataaac tgtggtttac cacgcttgat gactacaccc tgcacgcaga
     5221 ggtgcgcgtc gaaggtgaaa cgctcgcgac gcagcagatt aaactgcgcg acgttgcgcc
     5281 gaacagcgaa gcccccttgc agatcacgct gccgcagctg gacgcccgcg aagcgttcct
     5341 caacattacg gtgaccaaag attcccgcac ccgctacagc gaagccgggc attctatcgc
     5401 cacttatcag ttcccgctga aggaaaacac cgcgcaacca gtgcctttcg caccaaataa
     5461 tgcgcgtccg ctgacgctgg aagacgatcg tttgagctgc accgttcgcg gctacaactt
     5521 cgcgatcacc ttctcaaaaa tgagtggcaa accgacatcc tggcaggtga atggcgagtc
     5581 gctgctgacc cgcgagccaa agatcaactt cttcaagcca atgatcgaca accacaagca
     5641 ggagaacgaa gggctgtggc aaccgaatca tttgcagatc atgcaggaac atctgcgcga
     5701 ctttgccgta gaacagagcg atggtgaagt gttgatcatc agccgcacag ttattgcccc
     5761 gccggtgttt gacttcggga tgcgctgcac ctacatctgg cgcatcgctg ccgatggcca
     5821 ggttaacgtg gcgctttccg gcgagcgtta cggcgactat ccgcacatca ttccgtgcat
     5881 cggtttcacc atggggatta acggcgaata cgatcaggtg gcgtattacg gtcgtggacc
     5941 gggcgaaaac tacgccgaca gccagcaggc taacatcatc gatatctggc gcagcaccgt
     6001 cgatgccatg ttcgagaact atcccttccc gcagaacaac ggcaaccgtc agcatgtccg
     6061 ctggacggca ctgactaacc gccacggcaa cggtctgctg gtggttccgc agcgcccaat
     6121 taacttcagc gcctggcact atacccagga aaacatccac gctgcccagc actgtaacga
     6181 gctgcagcgc agtgatgaca tcaccctgaa cctcgaccac cagctacttg gcctcggctc
     6241 caactcctgg ggcagcgagg tgctggactc ctggcgcgtc tggttccgtg acttcagcta
     6301 cggctttacg ttgctgccgg tttctggcgg agaagctacc gcgcaaagcc tggcgtcgta
     6361 tgagttcggc gcagggttct tttccacgaa tttgcacagc gagaataagc aatgaggatc
     6421 atcgataact tagaacagtt ccgccagatt tacgcctctg gcaagaagtg gcaacgctgc
     6481 gttgaagcga ttgaaaatat cgacaacatt cagcctggcg tcgcccactc catcggtgac
     6541 tcattgactt accgcgtgga gacagactcc gcgaccgatg cgctatttac cgggcatcga
     6601 cgctattttg aagtgcatta ctacctgcaa gggcagcaaa aaattgaata tgcaccgaaa
     6661 gagacattac aggtagtgga atattatcgt gatgaaactg accgtgaata tttaaaaggc
     6721 tgcggagaaa ccgttgaggt ccacgaaggg caaatcgtta tttgcgatat ccatgaagcg
     6781 tatcggttta tctgcaataa cgcggtcaaa aaagtggttc tcaaagtcac catcgaagat
     6841 ggttatttcc ataacaaata acaactacgg cggcaaaagg agtttgccgc caccgctacc
     6901 ctacacattt tcggagatgt gttatgtctg ataccaaacg taatacaatc ggcaaattcg
     6961 gcttgctctc gctgactttt gccgccgttt acagctttaa caacgttatc aataataata
     7021 ttgagcttgg actggcctcg gcaccgatgt ttttcctcgc gacgattttt tattttattc
     7081 ccttctgtct gatcatcgca gaatttgttt cgttaaataa aaactcagaa gccggtgtct
     7141 acgcgtgggt aaaaagttcg ctgggcggac gttgggcatt tattactgcc tatacctact
     7201 ggttcgtaaa cctgttcttt ttcacctcac tgttgccgcg cgttattgct tatgcttcgt
     7261 atgccttcct cggatacgaa tatattatga cgccggttgc caccaccatt atcagtatgg
     7321 tgctgttcgc cttctccacc tgggtttcca ccaacggggc gaaaatgttg gggccaatta
     7381 cctccgtcac ttcaacgctg atgctgctgt taacgctctc ctacatttta ctggcaggta
     7441 cggcgctggt tggcggcgta cagcctgctg accccatcac cgttgacgcg atgatcccga
     7501 acttcaactg ggcgttcctc ggcgttacca cctggatctt tatggccgca ggtggcgcgg
     7561 agtccgtcgc tgtgtacgtt aacgacgtca aaggcggttc gaaatcgttc gttaaagtga
     7621 tcatcctcgc cgggattttt atcggcgtac tgtattccgt ctcctcggtg ctgattaacg
     7681 tcttcgtcag cagcaaagag ttgaaattta ccgtcggatc ggtgcaggta ttccacggca
     7741 tggcggcgta ttttggtcta ccggaagcgt tgatgaatcg ctttgtcggt ctggtgtcct
     7801 ttaccgcgat gttcggttcc ctgctgatgt ggaccgcaac gccggtgaaa attttcttct
     7861 ccgaaatccc ggaaggcatc tttggtaaga aaaccgtcga actgaacgaa aacggcgttc
     7921 cggcgcgcgc agcgtggatc cagttcctga tcgtcatccc gctgatgatt atcccgatgc
     7981 tcggttccaa taccgtgcag gatctgatga atactattat taatatgacc gccgcagcgt
     8041 ccatgcttcc gccgttattc atcatgctgg cttacctgaa tttacgcgcc aaattagatc
     8101 acctgccacg cgatttccgt atgggctccc gccgcaccgg tattatcgtt gtttcaatgc
     8161 tgattgcgat atttgccgta gggtttgtcg cttcgacatt cccgactggc gcgaatattc
     8221 tgaccatcat tttttataac gtcggcggta ttgttatctt ccttggcttt gcgtggtgga
     8281 aatacagtaa atatataaag ggattaacgg ctgaagagcg ccatattgaa gcgacgccag
     8341 ccagcaatgt tgattaagca gaataataaa aaatgagcag gcattttccc tcttcctgga
     8401 tgaggagagg gaaaatagtt tctgccttta tatttttata attacaacga taaaaggctg
     8461 tattttttct ttagctcatg gattaacaca atgaaattaa tcactgcacc atgcagagca
     8521 ttacttgctc tgccgttttg ctacgccttt tctgcggcag gagaagaagc acgtccggca
     8581 gaacatgacg acacaaaaac acctgcaatt acctcgacat cttctccttc atttcgtttt
     8641 tacggcgaat taggggttgg tggatatatg gatttagagg gtgagaataa acataaatac
     8701 agcgacggta cctatattga aggtggcctg gagatgaagt acggctcctg gttcggcctg
     8761 atttacggcg aaggctggac cgtgcaggcc gaccacgacg gcaatgcctg ggtgccagac
     8821 catagctggg gtggtttcga gggcggaatt aaccgtttct atggcggtta tcgtaccaat
     8881 gatggcaccg aaatcatgct cagtctgcgt caggattcct cgctggatga cctgcaatgg
     8941 tggggcgact tcacccccga tctgggctac gtcattccca atacccgcga cattatgacc
     9001 gcgctgaagg tgcagaactt aagcggcaac tttcgttata gcgtcaccgc cacacctgcc
     9061 ggacatcatg acgaaagcaa agcctggcta cattttggca agtacgatcg ctatgacgac
     9121 aaatacacct atccggcaat gatgaacggt tacatccagt atgaccttgc cgaaggcatc
     9181 acctggatga acggtctgga aatcaccgac ggcacaggac agctctatct cacgggcctg
     9241 ctaactccta actttgccgc tcgcgcctgg caccataccg gacgcgccga cgggctggac
     9301 gtaccgggaa gtgaaagtgg gatgatggtg agcgccatgt atgaagcgtt aaagggcgtt
     9361 tatctctcca ccgcttacac ctacgccaaa catcgccctg accacgctga cgatgaaacc
     9421 acctctttca tgcagtttgg tatctggtac gaatacggcg gcggacgttt cgccacggct
     9481 tttgatagcc gcttctacat gaaaaatgcc tctcacgatc ccagcgacca aatcttcctg
     9541 atgcaatatt tctactggta ataaggactg taatttcatg aaaataaaaa ctattttaac
     9601 gccagtaacc tgcgctctgc tgataagttt ttccgcccat gccgctaacg ccgacaatta
     9661 taaaaacgtg attaaccgta ctggcgcgcc gcagtacatg aaggattacg attacgacga
     9721 tcaccagcgt tttaatccgt ttttcgatct cggagcctgg catggtcatc tgttgccaga
     9781 cggccctaac accatgggcg gctttccggg cgttgcgctg ctgacggaag agtacatcaa
     9841 ctttatggcc agcaatttcg accgcctgac cgtctggcag gacggcaaga aagtcgactt
     9901 tacgctggag gcatacagta ttcccggtgc gctggtgcaa aaactgacag caaaagatgt
     9961 gcaagtcgaa atgactctgc gcttcgccac gccgcgcacg tcactactgg aaaccaaaat
    10021 caccagcaat aaaccgctgg atctggtgtg ggacggcgaa ctgctggaaa aactggaagc
    10081 gaaagaaggg aaaccgcttt ccgataaaac cattgctggc gaataccctg actaccagcg
    10141 caaaatcagc gccacccgtg atggcctgaa agtcaccttt ggcaaagtgc gcgccacctg
    10201 ggatctgctg acctccggcg aatcggaata tcaggtgcat aaatccctgc cggtgcagac
    10261 tgaaatcaac ggcaatcgct ttaccagtaa ggcacatatc aacggttcga cgacgctcta
    10321 taccacctat tcccatctgc tgacctctca ggaagttagc aaagagcaaa tgcagatccg
    10381 cgatattctg gcgcgtccgg cgttttatct caccgcctcg cagcaacgct gggaagagta
    10441 tctgaagaaa gggttaacca atccggatgc gacgccggaa cagacgcgcg tcgcggtgaa
    10501 agcgatcgaa acgctcaacg gtaactggcg ctcaccgggc ggtgcggtga aatttaacac
    10561 cgttacaccg tcggtgaccg ggcgctggtt ctccggcaat cagacctggc cgtgggatac
    10621 ctggaagcag gcgtttgcga tggcgcattt caatccggac atcgccaaag agaatatccg
    10681 cgcggtcttc tcctggcaga tccagcctgg cgatagcgtg cgtccgcagg atgtgggctt
    10741 tgtccccgac ctgatagcgt ggaaccttag ccccgagcgt ggtggcgatg gcggcaactg
    10801 gaacgaacgt aataccaaac ccagccttgc cgcctggtcg gtgatggaag tgtacaacgt
    10861 cacccaggat aaagcctggt tggcagagat gtacccgaaa ctggtggcct atcacgactg
    10921 gtggttacgt aaccgcgatc acaacggcaa cggcgtgccg taatatggcg cgacccgcga
    10981 caaagcccac aacactgaga gcggcgagat gctgtttacg gtgaagaaag gcaacaaaga
    11041 agagacgcag tctgggctga acaactacgc ccgcgtggtg gagaaaggcc agtacgacag
    11101 tctggaaatt ccggcacagg tcgctgcgtc gtgggaatcg gggcgtgatg atgccgccgt
    11161 ctttggtttt atcgacaaag aacagttgga taaatatgtc gctaatggcg gcaaacgtag
    11221 cgactggacg gtgaaattcg ccgaaaaccg cagtcaggac ggaacgttgc tgggctactc
    11281 gctattgcag gagtcggtgg atcaggccag ctatatgtac agcgataacc attatctggc
    11341 ggagatggca accatcctcg gtaagccgga agaggccaaa cgctatcgcc agttggcaca
    11401 gcagctcgca gactacataa acacctgtat gttcgacccg gctacccagt tctactatga
    11461 cgtgcgtatt gaagataaac cgctggcgaa cggctgcgcg ggcaaaccga ttgtcgagcg
    11521 cggtaaaggg ccggaaggct ggtcgccgct gtttaacggt gcggcaacgc aggccaacgc
    11581 cgacgcggtg gtgaaggtga tgctcgatcc taaagagttc aacacctttg tcccgctggg
    11641 aacggcggcg ttaaccaatc cggcttttgg cgctgatatc tactggcgcg ggcgcgtatg
    11701 ggtggatcag ttctggtttg gtctgaaagg gatggagcgt tacggttatc gtgatgatgc
    11761 cctgaaactg gcggatacgt tcttccgtca cgccaaagga ttaaccgccg atggcccaat
    11821 tcaggagaat tacaacccgc tgactggcgc acagcaaggc gcaccaaatt tctcctggag
    11881 tgccgcgcat ttgtatatgt tgtataacga ttttttccgt aagcaataat ttgcaactgc
    11941 tggcggatgc ggcgtaaacg ccttatccgc cctacatgtg caatcctcct ggccggataa
    12001 aacgcgacaa gcgtcgcatc cggcatctgc accgtgccac tggcggatgc ggcgtgaacg
    12061 ccttatccgc cctacatgtg caatccccca ggccggataa aacgcggcaa gcgtcgcatc
    12121 cggcgttacc accgggcgta ttctttttga atcccatcac aaaccccgca ctcccctttt
    12181 cccttttctc cggcgacggc taaattagaa ctcatccgac cacataacaa ttattttaca
    12241 tactggacaa ttttatgagc tacccgttgc tgttcgcccc gctggattta ggttttacca
    12301 cgttaaaaaa ccgcgtgttg atgggctcaa tgcacaccgg gctggaggaa tacccggacg
    12361 gtgccgagcg gctggcagcg ttttatgccg aacgcgcccg tcacggcgtg gcgctgattg
    12421 tcagcggcgg cattgcacca gatttaacag gcgttggcat ggaaggtggc gcaatgctca
    12481 acgacgccag ccagatccca caccatcgca ccattaccga agcggtacat caggaaggcg
    12541 gcaaaatagc cctgcaaatt ttgcataccg ggcgctacag ctaccaaccg catctggtcg
    12601 cttcctccgc attgcaggcc cccatcaacc gtttcgttcc tcacgaactc acccatgaag
    12661 agatcctgca actgatcgac gatttcgccc actgcgcgca actggcacgg gaggcaggat
    12721 acgacggtgt agaggtaatg gggtccgaag ggtatttgat caacgaattt ctgacgctgc
    12781 gcaccaatca gcgtagtgac cagtggggcg gcgattaccg caaccggatg cgatttgccg
    12841 tagaagtagt gcgtgcggtg cgcgaacgcg tcggcaacga cttcattatt atctaccgac
    12901 tgtcgatgct cgacctggtc gaagacggcg ggacttttgc cgaaacggta gagctggcgc
    12961 aggccattga agcggcgggc gcgaccatta tcaacaccgg cattggctgg catgaagcac
    13021 gtattccgac cattgccacg cccgtgccgc gcggcgcatt tacttgggtc acgcgcaaac
    13081 tgaaaggcca cgtttcgctg ccgctggtga ccaccaaccg gattaacgat ccgcaggttg
    13141 ccgacgacat tctctcgcgc ggcgatgccg atatggtatc gatggcacga ccgtttcttg
    13201 ctgatgcgga actgctgtca aaagcgcaat cggggcgagc cgatgagatc aacacctgca
    13261 ttggctgcaa tcaggcctgc ctcgatcaga tcttcgttgg caaagtcacc tcgtgcctgg
    13321 tgaatcctcg cgcctgccac gaaaccaaaa tgccaatcct tcctgccgtg cagaaaaaaa
    13381 atctggcggt ggtcggtgcg ggacctgctg ggctggcgtt tgccattaac gcggcggcgc
    13441 gtgggcatca tgtaacattg tttgatgccc acagcgagat cggcgggcag tttaatatcg
    13501 ccaaacagat ccccggcaaa gaggagtttt acgaaacgct gcgctattac caccggatga
    13561 tcgaagtgac gggcgtgacg ctgaaactca atcacaccgt gacggcggat cagttacagg
    13621 cgttcgatga aacgatcctc gccagtggga tcgtgccgcg cgttccgcct atcgacggga
    13681 tcgatcatcc gaaggtattg agttatctcg atgtactgcg cgacaaagcg ccggtaggca
    13741 acaaggttgc catcatcggt tgtggcggga ttggttttga tacggcgatg tatttaagtc
    13801 agccgggcga atccaccagc cagaatatcg ccaggttctg taatgagtgg gggatcgaca
    13861 gcagcctaca acaggctggt ggcttaagcc cgcagggaat gcagatcccg cgtagcccac
    13921 ggcaaattgt gatgctccag cgcaaagcca gcaaaccggg acaggggtta ggcaaaacca
    13981 ccggctggat ccatcgcacc accctgctct cgcggggtgt gaaaatgatc ccaggcgtaa
    14041 gttatcagaa gattgacgat gacgggctgc atgtggtgat caacggcgaa acgcaggtat
    14101 tagcagtgga caatgtggtg atctgcgcag ggcaagagcc aaaccgcgcg ctggcgcaac
    14161 cgctgattga tagcgggaaa actgtgcatt taattggcgg ctgcgatgtg gctatggagc
    14221 tggacgcacg acgggcaatt gcccagggaa cacggctggc gctggagatt taaatcgtta
    14281 tttgaagc
//
LOCUS       DAZFJF010000110        14100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFJF010000110 DAZFJF010000000
VERSION     DAZFJF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.110"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(91..1449)
                     /gene="plaP"
                     /locus_tag="ACHKAU_003755"
     CDS             complement(91..1449)
                     /gene="plaP"
                     /locus_tag="ACHKAU_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO5532737.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1439..1501)
                     /gene="yoeI"
                     /locus_tag="ACHKAU_003756"
     CDS             complement(1439..1501)
                     /gene="yoeI"
                     /locus_tag="ACHKAU_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO5532738.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1716..2645)
                     /gene="yeeY"
                     /locus_tag="ACHKAU_003757"
     CDS             complement(1716..2645)
                     /gene="yeeY"
                     /locus_tag="ACHKAU_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5532739.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2691..3515)
                     /gene="yeeZ"
                     /locus_tag="ACHKAU_003758"
     CDS             complement(2691..3515)
                     /gene="yeeZ"
                     /locus_tag="ACHKAU_003758"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5532740.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3598..3852)
                     /gene="yoeB"
                     /locus_tag="ACHKAU_003759"
     CDS             complement(3598..3852)
                     /gene="yoeB"
                     /locus_tag="ACHKAU_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO5532741.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3849..4100)
                     /gene="yefM"
                     /locus_tag="ACHKAU_003760"
     CDS             complement(3849..4100)
                     /gene="yefM"
                     /locus_tag="ACHKAU_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO5532742.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4383..4430
                     /gene="hisL"
                     /locus_tag="ACHKAU_003761"
     CDS             4383..4430
                     /gene="hisL"
                     /locus_tag="ACHKAU_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO5532743.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    4406..4529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4576..5475
                     /gene="hisG"
                     /locus_tag="ACHKAU_003762"
     CDS             4576..5475
                     /gene="hisG"
                     /locus_tag="ACHKAU_003762"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO5532744.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5481..6785
                     /gene="hisD"
                     /locus_tag="ACHKAU_003763"
     CDS             5481..6785
                     /gene="hisD"
                     /locus_tag="ACHKAU_003763"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO5532745.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6782..7852
                     /gene="hisC"
                     /locus_tag="ACHKAU_003764"
     CDS             6782..7852
                     /gene="hisC"
                     /locus_tag="ACHKAU_003764"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO5532746.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7852..8919
                     /gene="hisB"
                     /locus_tag="ACHKAU_003765"
     CDS             7852..8919
                     /gene="hisB"
                     /locus_tag="ACHKAU_003765"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO5532747.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8919..9509
                     /gene="hisH"
                     /locus_tag="ACHKAU_003766"
     CDS             8919..9509
                     /gene="hisH"
                     /locus_tag="ACHKAU_003766"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO5532748.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9509..10246
                     /gene="hisA"
                     /locus_tag="ACHKAU_003767"
     CDS             9509..10246
                     /gene="hisA"
                     /locus_tag="ACHKAU_003767"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO5532749.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10228..11004
                     /gene="hisF"
                     /locus_tag="ACHKAU_003768"
     CDS             10228..11004
                     /gene="hisF"
                     /locus_tag="ACHKAU_003768"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO5532750.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10998..11609
                     /gene="hisIE"
                     /locus_tag="ACHKAU_003769"
     CDS             10998..11609
                     /gene="hisIE"
                     /locus_tag="ACHKAU_003769"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO5532751.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11706..12683)
                     /gene="wzzB"
                     /locus_tag="ACHKAU_003770"
     CDS             complement(11706..12683)
                     /gene="wzzB"
                     /locus_tag="ACHKAU_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO5532752.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12830..13996)
                     /gene="ugd"
                     /locus_tag="ACHKAU_003771"
     CDS             complement(12830..13996)
                     /gene="ugd"
                     /locus_tag="ACHKAU_003771"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO5532753.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTKNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3570 a         3625 c         3584 g         3321 t
ORIGIN      
        1 aatggttatt aatgaggatt tcgataaaaa agaataaaaa aaccggagcc aaatgttcat
       61 tcgactccgg tttattatta gaaaagatga ttatgctacg tcttcgtact gcggaaccgg
      121 attacggaaa ctcttggtta cgcaagccag gtaaatcaga ccgatagcag cccagatcag
      181 acccagaacc attgagcttt cttccaggtt aacccacagc gcaccaacgg tcagcgcacc
      241 acacatcggc aggaacagat actggaagtg atctttcagc gtcttgttac gcttctcacg
      301 gatccagaac tgcgaaataa ccgacaggtt aacgaaggtg aacgccacca gcgcaccaaa
      361 gttaatcagc gccgtagcca ttaccaggtc gaagttgatt gccagcaagg caatcgcgcc
      421 aaccaggatg atgttcattg ccggagtacg ccatttcggg tgtacataac cgaagaagct
      481 tttcgggaac acaccgtcac gccccattac gtacatcaga cgcgctacgc ctgcatgcgc
      541 cgccataccg gacgccagta cggtaatggt ggagaagatc agcgcgccaa cctggaacgc
      601 tttacccgca acgtacagca tgatttcagg ctgtgacgca tccggatctt taaagcgaga
      661 gatatccggg aagtacagct gcaggaagta agttgcaaag atgaagatca tgccgccaat
      721 cagtgcggtc aggaaaatcg cacgcgggat cacgcgctct gcatctttgg tttcttccga
      781 caggttgctg atgccgtcaa agccggtaaa ggagaagcac aggattgtcg ccccggtaat
      841 catcgggata acatgcgcat cgccagacca gaacggacga gtgctcgcca gcgtacccgc
      901 gccttcacct tcaaatacgc cataaacaac catgcccaga atcaccgcga tcagcactac
      961 ctgcaacacg acgattacag tgttgaagtt cgctacggat ttcagactac gcaggttaaa
     1021 ggcggtcata aaggccacca gcgccaccac aaacatccac gatgggatgg aaggcaccag
     1081 agcttcaaaa tagattttcg ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag
     1141 cagagaagac caacccacca taaagccgac agtcgggcta atggatttct gggcgtaagt
     1201 gtatgcagag ccagcagaag gatagcggcg aaccagcttc ccgtagctca gagccgtaaa
     1261 caggatcgca atcaatgcga acgcataggc tgtcggcaca tgaccatccg tgaggcctga
     1321 aacgatacca aatgtatcaa acagcgtcat cggctgcata taggcaagac ccatcattac
     1381 aaccggaact aacgtaagcg ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt
     1441 atgcgacatg gtcattctcc tttacggtga taaccgtcgc gtaagcaaaa aattgcccca
     1501 tttttttgga ttcctcagcg acaacaactg tcgattttta gtaaatatct atccggtacg
     1561 aagcccggcc tcttggtatg aatgattggt ttgaagcaaa aaataaccga cgctaattaa
     1621 acgtcggttt ttagtcattt tttgacagcg gcgcattgtg cctaaatggg ggggaaatga
     1681 caagagaatg agaggcttgt cataataatt tttctttaaa tggctgattt tccgtcatca
     1741 gattttgcat aaacaccggc gatcgcacta tttgctaaaa tttcgtcccg ccaccaacca
     1801 ctggcaagcc ccgcggtctg ttcattccag ccaatccaga cgggttcaaa gagggtttgg
     1861 gcgaccactt tcttctcgat taacgcacca ctatcgagaa aacgttgcgc cagataacgg
     1921 ggtaaatagc cgcagccgag gccgctaatt tgcagctcca gcttggtttt aaaatcgaaa
     1981 acggtaatag cttcttgttc gtcgagaagt tgtgaggccg tagaagcgcc ggcctgtgca
     2041 gtgtccccca ccacaatcgc ccgatatcgt ttaatgatac gacggtttaa cggttcttct
     2101 tccagggcta acggatgatg aggcgcgacc gcaaagactt gttccagatc gcccaaccga
     2161 gagaaaccaa actcactgga agatggtggc tcatgcatcg cgccaacaat aatatccgct
     2221 cgcccctgag tcagggcatc ccatgaacca ccgagtacac cattgataaa cttcaggcgc
     2281 gtcacgctat gatgttgata aaaagcctca atgagtggcg caagcagaga aaaagggaag
     2341 gtgtcgtcca cgccaatcac cagctcgttt tcccagcctt catgcagttt gatcgcctgc
     2401 ttttccagtt ctcgcacggt atgcagaact tcccgccctt tctctaataa catttttccg
     2461 gtgcgggtga atttagcgcg gtgtccgctg cgatcaagca attgaatatt aaggtcgctt
     2521 tccagcttgt gaacggtgta actcagagcg gacggcgtct tgtagagttt ggccgacgcc
     2581 gccgcaaaac tgccttcttt ttctaaggca tcgaggatca tcaacacatc cagcagtggt
     2641 ttcatacacg cccccttgca gtgtatgcgg cgtcctgctg gcgtgatcgt ttactccagc
     2701 ggcattacca gcggatcggg atactggtat tcaaatccca gttcattaca aatccgactg
     2761 ccatcaataa tcttaccttt gccgctgtcc agactatttc tgaactgcgg aggttccagc
     2821 cccaataaac gggccatctg cggatagaaa acattacgcg cagggtgagc tggcgcacat
     2881 atattataga tgtgtccgcc tttaggtgcc tgtaacaaca gagtgatagc gccaataaca
     2941 tcttctaaat ggactaaatt aacaccatgt tcaccatcag gggcggtttt tccggcaaag
     3001 aagcgtccgg gatgacgtcc cggtccgacc aggcccgcaa gacgcagaat atcgaccgaa
     3061 gtaccgggta aattgtgcag ccagtcttcg agttcttcta atactcgtcc actgttggtc
     3121 accggattac gcggggtggt ttctttcacc gtgccttgcg cgtcgccata gacagatgtc
     3181 gagctggtaa aaataatgcg gggaatacga tgggccagcg cgctatccac taactcttgt
     3241 accgcttgta aatagaactc atcgccgggg ccgctacgac gtgccggaag cgtaatgacc
     3301 agcgcatcgg catccatcag ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc
     3361 atgcgaagca gatagctatc aatgccactc attcgggccg cttcgacacc atcttgtgtg
     3421 gttttactcc cggtgacttg ccagcctcgc gctgaaagtg acatcgccag cggcatgcct
     3481 aaccacccta acccgacaat tgcgaccttt ttcatccgtt atctcctgat acaccttaga
     3541 tctataaggc tacgctagcg tatcaaaact gacaattcat tctatgaatg aatctgttca
     3601 ataatgataa cgacatgctg caatgagcag tgaatcatca gtaaccgcgt ataccagacg
     3661 gtgctcctct gtaatgcgtc gggaccagaa acctgacaaa ttatgtttca ggggttctgg
     3721 tttcccctta ccttcaaatg gtgttctgcg ggtatctttg ataagttcat tgatcttttt
     3781 aacaattcgc ttatctgttt cctgccagta cagataatca tcccatgatt cctcagacca
     3841 gattagtttc actcaataat gtccttttcc gttcctttgc ctgatttcag gctatcgatt
     3901 gagtccatca atctccgggc gttagcgggg gaacgcagta gataagccgt ctcttccagc
     3961 gagttgtatt cttcgagtga catcagaaca caagcctctc cattctgacg agtaatgagg
     4021 atcggggcat gatcttcaac ggctttcatc attgttgccg acaaattctg acgcgcttcg
     4081 ctgtagctaa ttgtacgcat gtaaatctcc tcttttgtac agttcattgt acaatgatgg
     4141 gtgttaattc actatttatt aattagtttg tagatcaaga cattgccagc gagacgaaaa
     4201 atcagacttt gctctttttg gtgctgtcaa gttagaggac agtcctctta gccccctcct
     4261 tttcccgctc attcattaaa caaatccatc gccataaaat atataaaaaa gcccttgctt
     4321 tctaacgtga aagtggttta ggttaaaaga catcagttga ataaacattc acagagactt
     4381 ttatgacacg cgttcaattt aaacaccatc atcaccatca tcctgactag tctttcaggc
     4441 gatgtgtgct ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc
     4501 ggaagatcac cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta
     4561 aagaggaata agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc
     4621 cgtttaagtg atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac
     4681 acccagcgcc tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac
     4741 gatgacattc ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac
     4801 gtgctggaag aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc
     4861 ttgcgtcgtc tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc
     4921 tgggacggtc cgctctcctt aaatggtaaa cgtatcgcca cctcttatcc tcacctgctc
     4981 aagcgttatc tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggctctgtc
     5041 gaagttgcgc cgcgtgccgg actggcggat gcgatttgcg atctggtttc caccggtgcc
     5101 acgctggaag ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg
     5161 atccagcgcg atggcgaaat ggaagaatcc aaacagcaac tgatcgacaa actgctgacc
     5221 cgtattcagg gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc
     5281 gaacgtctgg atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg
     5341 ctggcgggtg accaacaacg cgtagcgatg cacatggtca gtagcgaaac cctgttctgg
     5401 gaaaccatgg aaaaactgaa agcgctgggt gccagttcaa tcctggtcct gccgattgag
     5461 aagatgatgg agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg
     5521 cggagcaaca acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc
     5581 gcaccgttaa cgatattctc gataacgtga aagcacgcgg cgatgacgcc ctgcgggaat
     5641 acagcgcgaa gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gaggagatcg
     5701 ccgccgccag cgaacgcctt agcgacgagc taaaacaggc gatggcggtg gcagtaaaaa
     5761 atattgaaac cttccacact gcgcaaaaac tgccgccggt agatgttgaa acacagccag
     5821 gcgtgcgttg ccagcaagtc acgcgcccgg tagcttcagt tgggttgtat attcctggcg
     5881 gctccgcccc actcttctca acggtattaa tgctggcaac tcctgcgcgt attgcgggct
     5941 gtaaaaaagt ggtgttgtgc tcaccgccgc cgattgccga tgagatcctt tatgcggcgc
     6001 agctgtgcgg tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgctctgg
     6061 cgtttggtac ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg
     6121 tcaccgaagc gaaacgtcag gtgagccagc gtcaggacgg tgcggcgatc gatatgcccg
     6181 caggcccgtc ggaagtgctg gtgattgctg acagcggcgc tacgccagat ttcgtggctt
     6241 ctgatttgct ttctcaggct gaacacggcc cggactcaca ggtgatttta ctgacgcccg
     6301 ctgctgatat ggcgcgtcgc gttgccgagg ccgtcgaacg ccagttggca gaactgccgc
     6361 gtgccgaaac cgcccgccag gcactgaacg ccagccgcct aatcgtgacc aacgatttag
     6421 cacagtgcgt ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca
     6481 acgctcgcga tctggtcgat agcatcacca gcgccggttc ggtatttctt ggtgactggt
     6541 caccggaatc ggcaggtgat tacgcctccg gcaccaacca cgttctgccg acttacggtt
     6601 acaccgccac ctgttccagc ctcgggctgg cagatttcca gaagcgcatg accgtgcagg
     6661 aactgtcgaa agaaggcttt tctgcgctgg cttcaaccat agaaacactg gccgccgccg
     6721 agcgcctgac cgcccataaa aatgccgtta ctttgcgtgt taacgccctt aaggagcaag
     6781 catgagcacc gtgactatta ccgatttagc gcgtgaaaac gtccgcaacc tgacgccgta
     6841 tcagtcggcg cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc
     6901 cgcagccgtg gagtttcagc ttactcaaca aacgctcaac cgctacccgg aatgccagcc
     6961 gaaagcggtg attgaaaatt acgcgcaata tgcaggcgta aaaccggagc aggtgctggt
     7021 cagccgtggc gcggacgaag gtattgaact gctgattcgc gctttttgcg aaccgggtaa
     7081 agacgccatc ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ccgaaacgat
     7141 tggcgtcgag tgtcgcacag tgccgacgca ggacaactgg caactggact tacagggcat
     7201 ttccgacaag ctggacggcg taaaagtggt ctatgtttgc agccccaaca acccgaccgg
     7261 gcaactgatc aatccgcagg attttcgcac tctgctggag ctaacccgcg gaaaagccat
     7321 tgtggttgcc gatgaagcct atatagagtt ttgtccacag gcatcgctgg ctggctggct
     7381 ggcggaatat ccgcacctgg ctattttgcg cacactatcg aaagcttttg ctctggcggg
     7441 gcttcgttgc ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat
     7501 cgccccctac ccgctctcga cgccggttgc cgacattgcg gcccaggcgt taagcccgca
     7561 ggggatcgtc gccatgcgcg aacgggtggc gcaaattatt gcagaacgcg aatacctgat
     7621 tgccgcactg aaagagatcc cctgcgtgga gcaggttttt gactctgaaa ccaactacat
     7681 tctggcgcgc tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat
     7741 cttacgtgat cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac
     7801 ccgtgaagaa agccagcgcg tcattgacgc cttacgtgcg gagcaagttt aatgagtcag
     7861 aagtatcttt ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag
     7921 gtggaccgtt ttgacaaact cgcctttgaa ccgggcgtga tcccggagct gctgaagttg
     7981 caaaaagcgg gctacaaact ggtgatgatc actaatcagg atggtctggg aacacaaagt
     8041 ttcccgcagg cggatttcga tggcccgcac aacctgatga tgcagatctt cacctcgcaa
     8101 ggcgtacagt ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc
     8161 cgtaagccga aagtaaaact ggtggagcgt tatctcgctg agcaagcgat ggatcgtgcc
     8221 aatagttatg tgattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggtatt
     8281 aatggtttac gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact
     8341 aaacgagacc gttacgccca tgtagtgcgt aacaccaaag agacgcaaat tgacgtccag
     8401 gtgtggctgg atcgcgaagg tgacagcaag attaacaccg gcgttggctt ctttgatcac
     8461 atgctggatc agatcgccac ccacggcggt ttccgtatgg aaatcaacgt caaaggcgac
     8521 ctctatatcg acgatcacca cacagtcgaa gataccggcc tggcgctggg tgaagcgtta
     8581 aaaattgccc ttggcgacaa acgcggtatt tgccgctttg gttttgtgct accgatggac
     8641 gaatgccttg cccgctgcgc gctggatatc tctggtcgcc cgcacctgga atataaagcc
     8701 gagtttacct accagcgcgt gggcgatctc agcaccgaaa tgatcgagca cttcttccgt
     8761 tcgctctctt acaccatggg cgtgacgcta cacctgaaaa ccaaaggtaa aaacgatcat
     8821 caccgtgtag agagcctgtt caaagccttt ggtcgcaccc tgcgccaggc catccgcgtg
     8881 gaaggcgaca ccctgccctc gtcaaaagga gtgctgtaat gaacgtggtg atccttgata
     8941 ccggctgcgc caatctgaac tcggtgaagt ctgccattgc gcgccacggt tatgaaccca
     9001 aagtcagccg tgacccggac gtcgtgttgc tggccgataa actgttttta cccggcgtag
     9061 gcaccgcaca agcagcgatg gatcaggtgc gtgagcgcga actgtttgat ctcatcaaag
     9121 cctgtaccca accggtgctg ggaatctgct tagggatgca actgctgggg cggcgcagcg
     9181 aagagagcaa cggcgtcgac ttgctgggca tcattgacga agacgtgccg aaaatgaccg
     9241 actttgctct gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc
     9301 gcctgtttca gggaattgaa gacggcgcgt acttttactt tgttcacagt tacgcaatgc
     9361 cggtcaatcc gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac
     9421 aaaaagataa cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta
     9481 agttgctgaa aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg
     9541 cactgtggtg cgtctccatc agggcgatta cggcaaacag cgcgattatg gtaacgaccc
     9601 gctgccgcga ttgcaggatt acgccgcgca gggtgcagaa ttgttgcatc tggtggatct
     9661 gaccggggca aaagatccgg ctaaacgtca aatcccgctg attaaaaccc tggtggcggg
     9721 cgttaacgtg ccggtgcagg ttggcggtgg cgtgcgtagc gaagaagacg tggcggcgtt
     9781 actggaagca ggcgttgcgc gcgtggtggt tggctccacc gcggtgaaat caccagagat
     9841 ggtgaaaggc tggtttgaac gtttcggtgc cgatgcctta gtgctggcgc tggatgtccg
     9901 tattgacgag caaggcaaca agcaggtggc agtcagcggc tggcaagaga actcgggcgt
     9961 ttcactggaa caactggtgg aaagctatct gcccgtcggc ctgaaacatg tgctgtgtac
    10021 cgatatctcg cgcgacggca cgctggcagg ttctaacgtc tctttatatg aagaagtgtg
    10081 cgccagatat ccgcaggtgg cgtttcagtc ctccggcggt attggcgaca ttgatgatgt
    10141 ggcggccctg cgtggtactg gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg
    10201 taaattcacc gtgaaggagg ccatcgcatg ctggcaaaac gcataatacc gtgtctcgac
    10261 gtccgtgatg gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat
    10321 atcgtgccgc tggcaaaacg ctacgctgaa gaaggtgcag acgaactggt gttctacgat
    10381 atcaccgctt ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa
    10441 gtgatcgaca ttccgttctg cgtggcgggt gggattaagt ctctggaaga tgccgcgaaa
    10501 attctttcct ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca
    10561 ttaattactc gcctggcgga tcgttttggc gtgcagtgta ttgtggtggg tattgatacc
    10621 tggtacgacg ctgaaaccgg taaatatcat gtgaatcaat ataccggcga tgaaagccgc
    10681 acccgcgtca ctcagtggga aacactcgac tgggtacagg aagtgcaaaa acgcggtgcc
    10741 ggagaaatcg tcctcaacat gatgaatcag gacggcgtgc gtaacggtta cgacctcgag
    10801 cagctgaaaa aagtgcgaga agtttgccgc gtcccgctga ttgcctccgg tggcgcgggc
    10861 accatggaac acttcctcga agccttccgt gatgccgacg ttgacggcgc gctggcggct
    10921 tccgtattcc acaaacaaat aatcaatatt ggtgaattaa aagcgtacct ggcaacacag
    10981 ggcgtggaga tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa
    11041 ccgacggact gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ctaatgctgg
    11101 gctatatgaa cccggaagcc ttagacaaaa ccatcgaaag cggcaaagtc actttcttct
    11161 cgcgcactaa acagcgactg tggaccaaag gcgaaacgtc gggcaatttc ctcaacgtag
    11221 taaatattgc gccggactgc gacaacgaca cgctactggt actggcgaat cccatcgggc
    11281 caacctgcca caaaggcacc agcagctgct tcggcgacac cgctcaccag tggctgttcc
    11341 tgtatcaact ggaacaattg ctcgccgaac gcaaatctgc cgatccggaa acctcttaca
    11401 cagccaaact gtatgccagc ggcaccaaac gcattgcaca gaaagtgggc gaagagggcg
    11461 tggaaaccgc actagcagca acggtacatg accgctttga actcaccaac gaggcgtctg
    11521 acttgatgta tcacctgttg gtgttgttgc aggatcagga tctggattta acaacggtga
    11581 ttgagaacct gcgtaaacgg catcagtgag ttgcggggta agcggatgcg atgttgttgt
    11641 cgcatccggc aaaaaaaacg ggcaaggtgt caccaccctg cccgttttct ttaaaaccaa
    11701 aaagattact tcgcgttgta attacgcaga gcgttacgcc ccagcacaat ccccgcgcca
    11761 accatgccgc ccagcagcac cgccagaatc aaagtgattg cctttttcgg gctatcgcga
    11821 cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag atcatcaact
    11881 tttaagcttt cgatatccag caggttttga cgtgtctgat agtagtttgg tgagaacacc
    11941 aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc caaaaggaac
    12001 aacgtatctt gtgtcacatc ttcagtctgc tgaacctgtg gctttgtcac ctgcgcctga
    12061 ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg ctcctgcgca
    12121 actacttcct gagttttcag agaatcctgc aagtttttca tacgcagcgc gatgttgtct
    12181 ttcaggtctt ttcccagttc ttcatttacc tggtcgtcaa cttgctggat atacttcgcc
    12241 agctgcttct gagcaccttc aggtgtttca ccaacataag acaccgctaa aggtaatgac
    12301 tgatttttga cggtaggctc aatggtcagc ttttctgctt cttcctggtt atccagcgtt
    12361 tccgctaatg ctgagaatgc agtgctgtaa cgtccgataa gtgaagcctg aatgtcggag
    12421 actttcggta cagccgatcc gtagatcaca ttcatcgcgt tggtataacc agcgatttgc
    12481 cccacatcag gttgagtaac gatagctgtg gaagtccact tttctttggc aacggccaga
    12541 taccctatag ccagggcaat agcaacaatg acagagatga taattgtcat cttgccacgc
    12601 cacaactgca ccagtaaatc aatcaaatca atctgttccg ggtcatggtt ttgcccagaa
    12661 acattattat tttctactct catcattacc ctaacggacg aaataatttt aagtcgccat
    12721 agtttagcga taatcgccat gaatgctata ggataaatga taaaaataat gaattattac
    12781 aaagggaaca taagagaatc agggcgaaaa tcgccctgat aacaggatgt tagtcactgc
    12841 caaagagatc gcgggtgtag actttgtctg ccacatcctt aagctcttct gccatacggt
    12901 tggagataat tacgtcggct tgttgtttga aagtggcgag atcacgttcc agccgagagt
    12961 tgaagaatga gtcttctttc atcactggct cgtagatgat tacttcaaca cctttcgcct
    13021 tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgaa ccgctcttca
    13081 taatcaggcg ataaataccc accacatgcg gtttgcgtga caaaatggca tcggcaataa
    13141 aatctttacg cgtgcggttt gcatcgacaa ttgccgagat caggttattc ggcacagact
    13201 gatagttcgc cagtaactgc ttggtatctt tcggcagaca ataaccacca taaccaaacg
    13261 acggattgtt gtaatggttg ccaatacgcg gatcgagaca aacgccttcg ataatctggc
    13321 gagtattcag acctaaactt tctgcgtagc tatccagttc attaaagtac gctacgcgca
    13381 tcgccagata ggtattagcg aaaagtttaa tcgcttctgc ttcggttgag tcggtaaaca
    13441 gggtcgggat attttgctta atcgcgcctt cctgtaacag agcagcaaaa cgttctgcgc
    13501 gttctgaacg ctcaccgatg acaatacgtg aaggatggag attatcgtaa agggctttac
    13561 cctcacggag aaattccgga gagaaaataa tatttttagt acgatatttc ttatgcatcg
    13621 ctgcggtaaa gccaacgggt accgttgatt tgatgaccat aaccgcataa ggatttatct
    13681 ctactacgtc cttaattact gattctacac tcgatgtatt gaaataatta gtgttaggat
    13741 catagtcggt tggagtggcg atgatgacat aatcagcatc ccggtaggct tcatttttat
    13801 ctaatgtggc attaaagtgt attttatctg attgcaaaaa ctgctgaatt tccttatcaa
    13861 caataggaga tatccgatca ttcagcatag caacgcgtga cggtaaaata tctaatgcca
    13921 caacctcatg attttgtgcg attagaagcc cgtttgacaa gcctacatag ccggtaccgg
    13981 aaatggtgat tttcatttca tgctctcaga attaacttaa ctgtgaatca tgatgttttt
    14041 agcatcctga taagagctaa aagttttaac gctacgttgt tcgatggtca acgcaacgaa
//
LOCUS       DAZFJF010000111        13699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFJF010000111 DAZFJF010000000
VERSION     DAZFJF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.111"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..882
                     /locus_tag="ACHKAU_003772"
                     /pseudo
     CDS             1..882
                     /locus_tag="ACHKAU_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            complement(1036..2685)
                     /gene="yjcE"
                     /locus_tag="ACHKAU_003773"
     CDS             complement(1036..2685)
                     /gene="yjcE"
                     /locus_tag="ACHKAU_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5532754.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2837..4186)
                     /gene="ghxP"
                     /locus_tag="ACHKAU_003774"
                     /gene_synonym="yjcD"
     CDS             complement(2837..4186)
                     /gene="ghxP"
                     /locus_tag="ACHKAU_003774"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO5532755.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4732..5196)
                     /gene="soxR"
                     /locus_tag="ACHKAU_003775"
     CDS             complement(4732..5196)
                     /gene="soxR"
                     /locus_tag="ACHKAU_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO5532756.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5282..5605
                     /gene="soxS"
                     /locus_tag="ACHKAU_003776"
     CDS             5282..5605
                     /gene="soxS"
                     /locus_tag="ACHKAU_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO5532757.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5608..7194)
                     /gene="pdeC"
                     /locus_tag="ACHKAU_003777"
     CDS             complement(5608..7194)
                     /gene="pdeC"
                     /locus_tag="ACHKAU_003777"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO5532758.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7624..7905
                     /gene="yjcB"
                     /locus_tag="ACHKAU_003778"
     CDS             7624..7905
                     /gene="yjcB"
                     /locus_tag="ACHKAU_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO5532759.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(8004..8540)
                     /gene="ssb1"
                     /locus_tag="ACHKAU_003779"
     CDS             complement(8004..8540)
                     /gene="ssb1"
                     /locus_tag="ACHKAU_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO5532760.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8794..11616
                     /gene="uvrA"
                     /locus_tag="ACHKAU_003780"
     CDS             8794..11616
                     /gene="uvrA"
                     /locus_tag="ACHKAU_003780"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO5532761.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11651..12007)
                     /gene="yjbR"
                     /locus_tag="ACHKAU_003781"
     CDS             complement(11651..12007)
                     /gene="yjbR"
                     /locus_tag="ACHKAU_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5532762.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12011..12427)
                     /gene="yjbQ"
                     /locus_tag="ACHKAU_003782"
     CDS             complement(12011..12427)
                     /gene="yjbQ"
                     /locus_tag="ACHKAU_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO5532763.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12538..13251)
                     /gene="aphA"
                     /locus_tag="ACHKAU_003783"
     CDS             complement(12538..13251)
                     /gene="aphA"
                     /locus_tag="ACHKAU_003783"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO5532764.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3599 a         3535 c         3304 g         3261 t
ORIGIN      
        1 gaaattccac agacagtaaa tggtggttac gatttttcac accttagcct gaaaggtatc
       61 gtgattaaag atgaagattt atccaattcg aattttgcag gttgctgact acaaaacgct
      121 attttccagg actgtaatat gtataaaacg aatttttatt acgccataat ggaaaaaata
      181 ctttttgata attgtattct cgatgactca aatttcgctc agataaaaat ggccgacgga
      241 actctaaatg catgctccgc tatgcatgtt caattctaca atgcagcaat gaatagagcc
      301 aatattaaaa atacctttct tgactattca aatttttata tggcgtacat ggctgaggta
      361 aatctttata aagtaatagc gccatatgtt aatttattta aagccgacct tagtttctct
      421 aaactcgatt taattaactt tgaacatgct gatctgtctc gcgtcaatct gaacaaagca
      481 atcctccaga atataaactt aattgatagc aaactctttt gtacgtggct aacaaataca
      541 ttcctcgaaa tggttatatg taccggctct aatatggcta atgttaattt taataatgcc
      601 aatttaagca actgccattt caactgttct attttaacaa aagcctgtat gtttaatacc
      661 cgtctctatc gggttaattt tgatgaggct agcgtccagg gaatgggcat ttctattctc
      721 cgtggggagg aaaatatccc cattgatagt gataccctgg taacactaca gaaattcttt
      781 gaagaagatt gtacctctca tactggcatg tcacaaactg aggataatat taatgcagtc
      841 gctatgaaga ttactgcaga tattatgcaa cacgcagatt gaggttaagt cgaagaacag
      901 ccaatgaatt gactttacaa gagagataac ttatttgttt tagaatcatg aatgttagcc
      961 cgagccaacc tgttaaaggt gcaaaagatt gcaccttttt cagattacct cgggcctggt
     1021 tacgtcgctt tcggcttact gattttcctc aattagcaac gcttcaagca aatcgagatc
     1081 gtgcagtaat ttttgcagcg tttcgttgct gatctcccgc gtggcgcgca ggtggtacag
     1141 ttcagcacgt tcagaacgca atgccgccag acggaagcga cgctcaaggt tctcttcctg
     1201 cacggaactt tcaacgtcgt tacgtccatc ggcgcgacga cgcaggttac caatgacgcg
     1261 agaactgacc tccgtaagca gctggttatc gatattctct tcggtatccg ccgccagacg
     1321 ctcctccatt ttctggatcg ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg
     1381 ttcttctttc aattgttgcg aatggtccgc gacttcaatg tgttgtagca gaataggcaa
     1441 catcaccacg ccgacaaaca gcgagaagag aatgacgcca gccgccagga acaccagctc
     1501 atagcgcgcc gggaagacgt taccatccgg caagagcagc gggatagaga gcacaccggc
     1561 cagagtgatc gccccacgca ccccggcgaa agacgcgatc aggatttctc gtgtggtcca
     1621 cgaaccaaac tccatcggct tcttcttcag gaagcggttg ctgaactttt tcatcgtcca
     1681 cagccagccg aaacggacca gcatcagcgc cgcatatatc agaataatat cggtaaacag
     1741 catccagatt tcgacgttag ggtcgatttc tgccgccatc agcgacgtct ccagaatacc
     1801 cggcagctgc agacctaaca gcaggaacac catgccgtta aagacaaatt ccagcatcgc
     1861 ccaggtgctg tttgcgcgca ggcgcattgc cagcggcgca cggcgcatca caccggagcg
     1921 ggtgatggtc atcccggcgg caaccgcagc gaggatgccg gaaacgccaa tatgttcggc
     1981 aatcagataa gaagcgaatg gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc
     2041 accgccccag cggctgagga atcgcagcga gcgaccatac agccagctca ccacaaagcc
     2101 ggcgagaata ccgccaatgg ctactttcat aaattcgacc gtcgcaccgc caacggtgaa
     2161 gatcatcgtc cccatcgcca ctgccacggc aaacttcaac gacaccaggc cggaggcgtc
     2221 attcatcaac gcttcgccct gcaaaatacc catgattttt ttcgggatgc gcccttcgcc
     2281 aacaatcccg gagagcgcca cagcatcggt cggagaaagc actgccgcca gcgcaaaggc
     2341 ggggatcagc ggaatgcccg gcaccaccca gtaaataagg aagccaatgc cgaccacggt
     2401 gaccaccacc agcgccagcg cgaggccaaa aatctctcga ccatgttcga gaaattcacg
     2461 ggtcggcgtt ttccagccat cagcgaacag caacggcggg ataaacaaga ctaaaaagag
     2521 ttcaggatca aactccacat gcaaaccaaa cgtcggccac gccagtagcg caccgatggc
     2581 gatttgcata agcggaagcg ggatctgaaa gggcatgaca cgagtgacca ccccggacag
     2641 cgagaccacg agggtcatta tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc
     2701 gatgtttttt tatgatctga gaaagacgtt atgcctgaat tctcattttt tcagaatatc
     2761 gcaaaagctc acacaatgtg cggagaaaat aaaaacgggc agccattgcc acccgttttc
     2821 agaatccgat gaaagattag atagcccagc cacccgcata gaaggtcacc agcgccacgg
     2881 cgataacgac cgtaccgatg ttcaacttgc gccattcgcc ggaaaccaga cgaccaatca
     2941 ccagagtcgc gaagccgatc atgatgcctg ttacgatgtt acaggtcagc acgatgaata
     3001 ccgccgtaac cagacccgcc atcgcatcaa caaaatcagc aaagtcgatt ttcgccacgt
     3061 tgctcagcat cagcaggcca acgtacatca gcgccggagc cgttgcatac cccggaacga
     3121 ggtaagagag cggagagagg aacagaatca gcaggaacag cacgccaacg gtgatagccg
     3181 tcaaaccggt tttaccgccc gccgccgtac ccgccgcaga ctcgatgtat accgctgccg
     3241 gagcagcacc caccaggcca gagaaaacgc tgctcatgga gtcagtggtc agtgctttcc
     3301 caccgtcgat gatctgccca tctttatcca gcaggttcgc ctggccggcg acggcacgga
     3361 tagttccggt ggcatcaaat actgccgtca tcaccagcgc cagaacgctt ggcaggacta
     3421 caggattcag cgcgcccata atgtccaggc tgccaatcag tgaattgccg ttttcatcgc
     3481 tcaatgaagg catggcgaaa acgccggaga aatggacgtt aggatcgaag atcaaaccga
     3541 caattgagat accgataatg gtcagcagaa tgccaccagg gactttcagt ttttccaggc
     3601 cgatgatcac cgccagacct actagtgaca taatcaccgg gaaggtcgcg aaatcaccca
     3661 gcgcaacggg cagaccatca agcgggtttt taatcaccag accgacacca ttagcggcaa
     3721 tgagcagcag gaacagaccg ataccgatcc ccgtgccgtg cgccacaccg tgcggcaagt
     3781 tgcgcaaaat ccagctacgg atacccgtgg cagaaattac cgtaaacagc acccccatca
     3841 ggaacacggc acccagcgcg acaggtacgc taatatgttg ccccagcacc aggctgaatg
     3901 cggtaaacgc cgtcagggag atggcgcaac caatcgccaa cggcagatta gcccacagac
     3961 ccatcacgat agaaccgagt ccggcaacca gacaggttgc aacgaaaact gccgcaggcg
     4021 ggaagcctgc tttacccaac atacctggaa cgacgatgac cgagtagacc atcgccagaa
     4081 acgttgttaa cccggcaacc acttcctgac ggacagtgct tccacgttgt gaaattttaa
     4141 accaggcgtc gagtgaaccg ccggtacgcg ctgatggcgt agacataaga aaacatcccc
     4201 tgagagttta attttcgtca agatgcgtgg cggacaatcc cctgccagcg aaacgttatc
     4261 ttcctgctcc aggtttgcga caatttactg cgtcgcaaaa aagcaaacgt ttagctccgc
     4321 gcatacaaaa cgggtggtaa aaaaagcaaa cgattatccg gcgatcatag cggctttttc
     4381 aaccgaagtt tgctgctttt ttctctttta ttgtcttgac gatgaaaaac caggcaattg
     4441 atgcgcaaac gttatcgtcg gtgcgcaaga ttgcagcgtt atcgagccgt ataccgttca
     4501 ggattgcgat aaaaaatcgt ctgagatgac aaaaatattg cggataaaaa gtatactttt
     4561 acactggcga agaaatgccg gtgacataca attaaagcat caacaccaac cggaacctcc
     4621 accacgtgct cgaatggggt gtgttgacgt cgggggaaac cctcctgtgt accagcggga
     4681 tagagagaaa gacaaagacc ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg
     4741 ttcatcttcc agcaagcgtg cgccggtacc ttcttctcct aagcggtcgc ccgggttacg
     4801 caacgggcaa tcactgcgcg aaaggcagcc acaaccaata catccgtcca gttcgtcacg
     4861 cagcgctact aaggtatgaa tgcgccgatc caactcttct cgccattggg acgaaagctg
     4921 tttccactct ttcgcactta acgtatgccc ttcgggcaac acgccaaacg cttcaccaat
     4981 ggtcgccagc ggaatgccaa tacgctgagc aattttgata attgcaacat atcgcaacac
     5041 atcacgttta tatcgccgct gattgccgct gttacggata ctggtaatca accctttact
     5101 ttcatagaaa tgcagcgccg ataccgccac accgctgcgt ttcgccactt cgccgggggt
     5161 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
     5221 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
     5281 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
     5341 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
     5401 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
     5461 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
     5521 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
     5581 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
     5641 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa aacatccctg
     5701 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
     5761 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
     5821 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
     5881 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
     5941 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttgtcaa catcaagaaa
     6001 tgcatgttca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
     6061 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
     6121 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcacggaaga cattatcaat
     6181 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     6241 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     6301 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     6361 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     6421 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     6481 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     6541 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     6601 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     6661 atcatacaca ccccattgca atgtcggatc atcagacatc acttcactcc agaagagagg
     6721 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     6781 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     6841 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     6901 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     6961 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     7021 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     7081 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     7141 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     7201 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     7261 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     7321 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     7381 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     7441 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagttc acaaaattaa
     7501 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     7561 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     7621 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcaacttct tagtgccgta
     7681 atgatgtttc tggccagcac actcaacatc cgttttcgtc ggtctgatta tgtcgggctt
     7741 gcggtgatca gcagcggtct gggcgttgtt tccgcctgtt ggttcgcaat ggggttgctt
     7801 ggcatcacaa tggcggacat caccgctatc tggcacaaca tcgagttggt gatgatagaa
     7861 gagatgaatc agacaccgcc acaatggcca atgattttga cctgatacgt agaagcctcc
     7921 ataacggagg cttctttttt atggctggca gatgctttaa tcatccacct taaaacaata
     7981 taacctattg ttttaatgac aaatcagaac ggaatgtcat catcaaagtc catcggcggc
     8041 tcgttagacg gcgctgccgg agcggactgc tgcgggcgag actgcgcgcc gccgctgaac
     8101 tgattgccac cctgcggctg ctgtggctga ccccaaccgc cctgcggctg accaccaccg
     8161 atattgccac ctgccggagc gccaccaccc tgacgaccac ccagcatctg catggtgccg
     8221 ccaacgttca ccacgacttc tgtggtgtag cgatcctgac cggattgatc ggtccattta
     8281 cgggtacgca gctgaccttc gatataaacc tgagaacctt tacgcagata ttcgctcgcc
     8341 acttctgcca gtttgccgaa cagcacaacg cggtgccatt cagtctgttc tttcatctcg
     8401 ccggtcgctt tatcacgcca ggattcggaa gtagccagcg taatgttggc aactgcgcca
     8461 ccatttggca tgtagcgtac ttccgggtcc tgacccagat taccaacgag aataaccttg
     8521 tttacgcctc tgctggccat gttcgtgtct cctgaaaaaa atcgttctga ataagtgtaa
     8581 acgcgcgatt gtaccattac caatagcgct tttactatgt tgtgacctcg gttccgggaa
     8641 acaaacctgg ccagacattg ttacacaaca ctccgggtaa tgcattccaa tactgtatat
     8701 tcattcaggt caatttgtgt cataattaac cgtttgtgat cgccggtagc accatgccac
     8761 cgggcaaaaa agcgtttaat ccgggaaagg tgaatggata agatcgaagt tcggggcgcc
     8821 cgcacccata atctcaaaaa catcaacctc gttatccccc gcgacaagct cattgtcgtg
     8881 accgggcttt cgggttctgg caaatcctcg ctcgctttcg acaccttata tgccgaaggg
     8941 cagcgccgtt acgttgaatc cctttccgcc tacgcgcggc agtttctgtc actgatggaa
     9001 aagccggacg tcgatcatat tgaggggctt tctcctgcca tctcaattga gcagaaatcg
     9061 acgtctcata acccgcgttc tacggtgggg acaatcaccg aaatccacga ctatttgcgt
     9121 ttgttgttcg cccgcgtcgg cgaaccgcgc tgtccggacc acgacgtccc gctggcggcg
     9181 caaaccgtca gccagatggt ggataacgtg ctgtcgcagc cggaaggcaa gcgtctgatg
     9241 ctgctcgcgc caatcattaa agagcgcaaa ggcgaacaca ccaaaacgct ggagaacctg
     9301 gcaagccagg gttacatccg tgctcgtatt gatggcgaag tctgcgatct ttccgatccg
     9361 ccgaaactgg aactgcaaaa gaaacatacc attgaagtgg tggttgatcg cttcaaggtg
     9421 cgtgacgatc ttacccaacg tcttgcggag tcgtttgaaa ccgcgctgga gctttccggt
     9481 ggtacagcgg tagtggcgga tatggacgac ccgaaagcgg aagagctgct gttctccgcc
     9541 aacttcgcct gcccaatttg cggctacagt atgcgtgaac tggagccgcg actgttttcg
     9601 tttaacaacc cggcaggtgc ctgcccgacc tgtgacggcc ttggcgtaca gcaatatttc
     9661 gatcctgacc gcgtgatcca aaaccccgag ctgtcactgg ctggcggtgc gatccgtggc
     9721 tgggatcgcc gcaacttcta ttacttccag atgctgaaat cgctggcaga tcactataag
     9781 ttcgacgtcg aagcgccgtg gggcagcctg agcgcgaacg tgcataaagt ggtgttgtac
     9841 ggttctggca aagaaaacat tgaattcaaa tacatgaacg atcgtggcga tacctccatc
     9901 cgtcgtcatc cgttcgaagg cgtgctgcac aatatggagc gccgttataa agagacagaa
     9961 tccagtgcgg tacgtgaaga attagccaag tttatcagca atcgcccatg cgccagctgc
    10021 gaagggacgc gtctgcgtcg ggaagcgcgc cacgtttatg tcgagaatac gccgctgcct
    10081 gctatctccg acatgagcat cggtcatgcg atggaattct tcaacaatct caaactcgct
    10141 ggtcaacggg cgaagattgc ggaaaaaatc cttaaagaga tcggcgatcg tttgaaattc
    10201 ctcgttaacg tcggcctgaa ttacctgacg ctttcccgct cggcagaaac gctttccggc
    10261 ggtgaagccc agcgtatccg tctggcgagc cagattggtg cgggcctggt tggcgttatg
    10321 tacgtgctgg acgagccatc tatcggcctg caccagcgcg ataacgagcg cctgttgggt
    10381 acgcttatcc atctgcgcga tctcggtaat accgtgattg tggtggagca cgacgaagac
    10441 gcaattcgcg ccgctgacca tgtgatcgac attggcccgg gcgcaggtgt tcacggcggt
    10501 gaagtggtcg cagaaggtcc gctggaagcg attatggcgg tgccggagtc gttgaccggg
    10561 cagtacatga gcggcaaacg caagattgaa gtgccgaaga aacgcgttcc ggcgaatccg
    10621 gaaaaagtgc tgaagctgac aggcgcacgc ggcaacaacc tgaaggacgt gacgctgacg
    10681 ctgccggtgg gtctgtttac ctgcatcacc ggggtttcag gttccggtaa atcgacgctg
    10741 attaacgaca cactgttccc gattgcccaa cgccagttga atggggcgac catcgccgaa
    10801 ccagcaccgt atcgcgatat tcaggggctg gagcatttcg ataaagtgat cgatatcgac
    10861 caaagcccaa ttggtcgtac tccacgttct aacccggcga cctataccgg cgtgtttacg
    10921 cctgtgcgcg aactgtttgc gggcgtaccg gaatcccgtg cgcgtggtta tacgccagga
    10981 cgtttcagct ttaacgtccg tggcgggcgc tgcgaagcct gtcagggcga cggtgtgatc
    11041 aaagtagaga tgcacttcct gccggatatc tacgtgccgt gcgatcagtg taaaggtaaa
    11101 cgctataacc gtgaaacgct ggaaattaag tacaaaggca aaaccatcca cgaagtgctg
    11161 gatatgacca tcgaagaggc gcgtgagttc tttgatgccg tacctgcact ggcgcgtaag
    11221 ctgcaaacgt tgatggacgt tggcctgacg tacattcgcc tggggcagtc cgcaaccaca
    11281 ctttctggtg gtgaagccca gcgcgtgaag ctggcgcgtg agctgtcaaa acgcggcacc
    11341 gggcagacgc tgtatattct cgacgagccg accaccggtc tgcacttcgc cgatattcag
    11401 caactgctcg acgtactgca taaactgcgc gatcagggca acaccattgt ggtgattgag
    11461 cacaatctcg acgtgatcaa aaccgctgac tggattgtcg acctgggacc agaaggcggc
    11521 agtggcggcg gcgaaatcct cgtctccggt acgccagaaa ccgtcgcgga gtgcgaagct
    11581 tcgcatacgg cacgcttcct caagccgatg ctgtaatcgt taaggccgct ttctgagcgg
    11641 ccttttcctt tcagagttgt accagcaatt tacgcttttc ttccggtagt aaattcactg
    11701 cctgctgata agacgcatcc accagataat agatttgcga atccggcagc gaaccgtcga
    11761 gatacacggt gctccagtgc gctttattca gatggcggct aggacgcaca tcgctgtgct
    11821 gctgacgtag cagctccgcc agctccgggc tggttttcag cgaaacagct gggcgatttt
    11881 ctacttcttt caccatcgca aacagtacat cttccacttt gatctgcgtc gctttccagt
    11941 cgttatgcac gctctgttct gcgcctggtt ttgccatgca atattgtagc aactccgaaa
    12001 tggtcatttt ttactcccct tgtagtgtcg cgatgatgcg acgcgatccg ccgtggatgc
    12061 gatgttcccc cagccagatg ccttgccagg tgccggtctg aatacgccct ttatgtaccg
    12121 gcaatacaag cgatgttccc agcattgagg atttgatatg agaaggcata tcgtctgctc
    12181 cctcatagtc atgctcataa tttccgttgt cgggaacggt gcggaggaaa aaacgctcca
    12241 tgtcgtggcg tacggtggga tcgcagttct cattaagtgt cagagaggcg gaggtatgtt
    12301 gcagcaacag atgcagtaag ccgatgttaa cgcgcggcat atcagccagc tgattcagaa
    12361 tttcatccgt taccagatga aacccacgag atttggcgct aagcgtgagc gtcttttgat
    12421 accacatgtg ctgctccttg ataaaactct cttaatcagt ttgcagcaag acagcgaaag
    12481 gataaaggtg tgattaaaaa aacagcattc gggagagcgt cacgctctcc cgctctgtca
    12541 gtattctgaa ttgacgatca cctcttcacc aaacgcaccc gcttgtggca agggtttgta
    12601 ggtagagttg gaggcgcgca gaatgcggat accacgagcg ccgacatcgc gtgcggcggt
    12661 aatatcatta tcagaatcgc cataaaaaat tcggatattt ttatcctgca gccattgcga
    12721 ttttgtattt tgccctagtt tatcgcccgc aaagatcacc ggattcatgt tggtggcagg
    12781 aatatgaaaa ttatccgcca gcgtttttga aaccgtttct gttttcgtcg gactacgacc
    12841 agtcacaaag aagatcgcgt caccgcggcg tacatgcata tcaatcagct ggcgagcgac
    12901 ctcttttgga atgctgaatt catcccagcc attgttcatt ttttcccaga acacaggatt
    12961 tttcagataa tcttcgcttt ctggcgagaa ggtttttttg ccgcgccaga agcccggact
    13021 ggaaaaaagt accgtgtcat cgatatcaaa ccccaccgcc attggcggac gccctgcgag
    13081 gctattttca atttgtgcga ccgaaaccca atgaatgggt gcctgttcag caagcctggc
    13141 aacgttagtc ccagggttaa gcggtgaagg agatgaggcc agggcaacag cggaactgtt
    13201 tagagcgaac aataagcaaa cggcactgat tgcctgtgtg atcttgcgca tatttttccc
    13261 taaatagtca gtatgttgaa actttttatt gtgagatttg ttgcaaaaac catctgacca
    13321 taacgccagc tgccggggaa aggaaggatt atctgcggtt tttgtgatga aaagcaggat
    13381 gattatattt aacccagtaa atacgttcct gtcttttttt gtataaaaaa tgtttattac
    13441 tcgatgtaag gatacattaa ttttacgttt attatgatga atcctcggca aatgagcgaa
    13501 atttgataga acatttatct gctgtacgat ttaatgaact taatgataca gcattacaaa
    13561 aacaatcgaa gtttataaag atgatttctg attgaccagc cccctaccct acaatggtga
    13621 ttaattttat tactcgcgat ggagatgatg atatgaatat atcatatgtg aatagcaaca
    13681 aaacgacatc tttacctgt
//
LOCUS       DAZFJF010000112        13648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFJF010000112 DAZFJF010000000
VERSION     DAZFJF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.112"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(14..202)
                     /gene="bcsR"
                     /locus_tag="ACHKAU_003784"
     CDS             complement(14..202)
                     /gene="bcsR"
                     /locus_tag="ACHKAU_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO5532765.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            475..2046
                     /gene="bcsE"
                     /locus_tag="ACHKAU_003785"
     CDS             475..2046
                     /gene="bcsE"
                     /locus_tag="ACHKAU_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO5532766.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            2043..2234
                     /gene="bcsF"
                     /locus_tag="ACHKAU_003786"
     CDS             2043..2234
                     /gene="bcsF"
                     /locus_tag="ACHKAU_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO5532767.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            2231..3910
                     /gene="bcsG"
                     /locus_tag="ACHKAU_003787"
     CDS             2231..3910
                     /gene="bcsG"
                     /locus_tag="ACHKAU_003787"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO5532768.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(3997..4104)
                     /locus_tag="ACHKAU_003788"
     CDS             complement(3997..4104)
                     /locus_tag="ACHKAU_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5532769.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(4480..4587)
                     /locus_tag="ACHKAU_003789"
     CDS             complement(4480..4587)
                     /locus_tag="ACHKAU_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5532770.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(4963..5070)
                     /locus_tag="ACHKAU_003790"
     CDS             complement(4963..5070)
                     /locus_tag="ACHKAU_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5532771.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(5446..5553)
                     /locus_tag="ACHKAU_003791"
     CDS             complement(5446..5553)
                     /locus_tag="ACHKAU_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5532772.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            6029..7300
                     /locus_tag="ACHKAU_003792"
     CDS             6029..7300
                     /locus_tag="ACHKAU_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO5532773.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(7330..8334)
                     /gene="dppF"
                     /locus_tag="ACHKAU_003793"
     CDS             complement(7330..8334)
                     /gene="dppF"
                     /locus_tag="ACHKAU_003793"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO5532774.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(8331..9314)
                     /gene="dppD"
                     /locus_tag="ACHKAU_003794"
     CDS             complement(8331..9314)
                     /gene="dppD"
                     /locus_tag="ACHKAU_003794"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO5532775.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(9325..10227)
                     /gene="dppC"
                     /locus_tag="ACHKAU_003795"
     CDS             complement(9325..10227)
                     /gene="dppC"
                     /locus_tag="ACHKAU_003795"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO5532776.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(10237..11256)
                     /gene="dppB"
                     /locus_tag="ACHKAU_003796"
     CDS             complement(10237..11256)
                     /gene="dppB"
                     /locus_tag="ACHKAU_003796"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO5532777.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(11407..13014)
                     /gene="dppA"
                     /locus_tag="ACHKAU_003797"
     CDS             complement(11407..13014)
                     /gene="dppA"
                     /locus_tag="ACHKAU_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO5532778.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(13366..>13648)
                     /locus_tag="ACHKAU_003798"
     CDS             complement(13366..>13648)
                     /locus_tag="ACHKAU_003798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532779.1"
                     /translation="SVASGNGTALIVGCGATALFDQHTTHFPANFAPHISLSINGFVT
                     ETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         3159 a         3665 c         3414 g         3410 t
ORIGIN      
        1 attcaccaat cccctacttt tgttgcgcaa actctgccag caacggccag cgttttaatg
       61 ccgcggccaa ctgttcgcgt tgggaaatat cggcataatc aatatcaggc agtgaaaatg
      121 cctgctttaa cgccacaata tcattctgga agatatagcc tatcgcggga tcaggcagag
      181 tatctggttc attgttattc attttgcttg atccctatga aataaccgta ccgttcatga
      241 gacaattcta accgtgagca tttcgccggt ttattgttta cggcgagacg ggaatttgct
      301 cactttttga ctttccgtta cgcaacagaa aaagttaaaa acgaaaagac taaaatcaca
      361 cgtcttgtga ccttctaaat tgattcacat gctaaatctg ataagtttta atttcaatgg
      421 taggtttatt tcttagcttt cgctagtaaa ctgataatca gacaaaatgg tgacatgagg
      481 gacattgtgg accctgtatt ctctatcggt atctcatcat tatgggatga gctgcgacat
      541 atgccagcag gcggcgtctg gtggtttaac gtcgatcgcc atgaagatgc tatcagtctg
      601 gcgaatcaaa caattgcatc ccaggctgaa accgcacacg tcgcggtcat tagcatggac
      661 agcgatccgg cgaaaatctt tcaattagat gattctcaag ggccggaaaa aataaaatta
      721 ttttcaatgc taaatcatga aaaaggtcta tactatttga cccgtgattt gcagtgttct
      781 attgatcccc ataattacct ttttattctt gtttgcgcaa ataatgcatg gcaaaacatt
      841 cctgccgagc ggcttcgctc atggttggat aaaatgaata aatggagcag gttaaaccat
      901 tgttcgcttt tggtaattaa tcccggaaat aataacgata aacaattttc attgttgctt
      961 gaggaatacc gttcactttt tggtcttgcc agtttgcgtt ttcagggtga ccaacatttg
     1021 ctggatattg ccttctggtg caacgaaaaa ggggtcagcg cccgtcagca gcttagcgtt
     1081 cagcaacaaa atggtatctg gacattagtt caaagcgaag aggcggagat ccaaccacgc
     1141 agcgacgaaa aacgcattct gagtaatgtt gctgtactgg aaggtgcgcc gccgctatcg
     1201 gaacactggc aactgttcaa caataacgaa gtcctgttca atgaagcccg taccgctcag
     1261 gcggcgacgg tggtcttttc tttacagcaa aatgcgcaaa tcgagccact ggcccgcagc
     1321 attcataccc tgcgtcgcca gcgcggtagt gcgatgaaaa tcctcgtgcg ggaaaatacc
     1381 gctagcctgc gcgccaccga tgaacgtttg ttattggcct gcggtgcaaa tatggttatt
     1441 ccgtggaatg cgccactctc ccgttgtctg acgatgatcg aaagcgtgca agggcagaag
     1501 tttagtcgct atgtgccgga agatatcact accttgctgt caatgaccca gccgctcaaa
     1561 ctgcgtggtt tccagaagtg ggatgtgttc tgtaatgccg tcaacaacat gatgaataac
     1621 cctctattac ctgcccacgg taaaggcgtt ctggttgccc tacgtccggt accgggtatc
     1681 cgcgttgaac aagccctgac gctgtgtcgc cctaaccgta ccggcgatat catgaccatt
     1741 ggcggtaatc ggctggtgct gtttctctca ttctgtcgga ttaacgatct ggataccgcg
     1801 ttgaatcata ttttcccatt gcctactggc gacattttct caaaccgtat ggtctggttt
     1861 gaagatgatc aaatcagtgc cgagctagtg cagatgcgct tgcttgcccc agaacaatgg
     1921 ggcatgccgc tgcctttaac gcaaagttct aaaccggtca tcaatgccga gcacgatggt
     1981 cgccactggc gacgaatacc agaacccatg cgactgttag atgatgctgt ggagcgctca
     2041 tcatgatgac catcagcgat atcattgaaa ttattgtcgt ttgcgcactg atatttttcc
     2101 cgctgggcta tctggcgcgg cactctttgc gacgcattcg cgacacctta cgtttgttct
     2161 ttgctaaacc tcgttatgtt aaaccggccg ggacgttacg ccgcacggaa aaagccaggg
     2221 caaccaaaaa atgactcaat ttacgcaaaa taccgccatg ccttcttccc tctggcaata
     2281 ctggcgcggc ctttccggct ggaacttcta ttttctggtt aagttcggcc tgttgtgggc
     2341 gggatatctt aacttccatc cgctcctcaa tttggtgttt gccgcgtttc tgctgatgcc
     2401 cattccgcgc tacagcctgc atcgcttgcg ccactggatt gccctgccga tcggctttgc
     2461 tttgttctgg catgacacct ggttgcctgg cccggaaagc ataatgagcc agggttcgca
     2521 ggtggcgggg ttcagtaccg attatttaat cgaccttgtc acacgcttta ttaactggca
     2581 gatgattggg gccatttttg ttttattagt ggcctggtta ttcctgtcac aatggattcg
     2641 cattaccgtt tttgtggttg ccatactgct atggctgaac gtacttaccc tggcgggacc
     2701 aagtttctcc ttgtggccag ccggacaacc gacgaccact gtaacaacga cgggtggtaa
     2761 cgcagcggca accgttgcgg cgacgggtgg cgcaccggta gtgagtgata tgcccgcaca
     2821 aactgcaccg ccaacaacgg cgaaccttaa cgcctggctg aataatttct ataacgcgga
     2881 ggcaaaacgt aaatcgacct tcccgtcttc gctgcccgct gatgctcagc catttgaact
     2941 actggtgatt aacatctgtt cgctttcctg gtcggatata gaagccgccg ggttgatgtc
     3001 gcatccactg tggtcgcatt tcgatattga gttcaagaac tttaactccg ccacctccta
     3061 cagtggcccg gcggcgatcc gtttactgcg cgccagctgc gggcagactt cgcacactaa
     3121 tctgtatcaa ccggcaaata acgactgcta tctgtttgat aacctttcga aactgggctt
     3181 tacccagcac ctgatgatgg ggcataacgg ccagttcggc ggttttttga aagaagttcg
     3241 cgaaaatggc ggcatgcaga ctgaattgat ggatcaaaca aatctgccgg ttattttgct
     3301 gggctttgat ggttcgccgg tttatgacga taccgccgtg cttaaccgct ggctggacgt
     3361 taccgaaaaa gataaaaata gccgtagtgc cacgttctac aacacgcttc cactgcatga
     3421 cggcaaccat tatccggggg tcagcaaaac agcggattac aaagcgcggg cgcagaaatt
     3481 ctttgatgaa ctggacgcct tctttactga actggagaaa tcgggtcgta aagtgatggt
     3541 ggtcgtggtg ccggaacacg gcggcgcgct gaagggcgac agaatgcagg tatctggcct
     3601 acgtgatatc cctagcccgt ctatcacaga cgtccccgtt ggggtgaaat tcttcggcat
     3661 gaaggcacca catcaggggg caccgattgt cattgaccaa ccgagcagct tcctggctat
     3721 ctccgatctg gtggttcgcg ttcttgatgg caagattttc accgaagaca atgttgactg
     3781 gaaaaaactc accagtgggt tgccacaaac agcaccggtc tccgagaact caaatgcagt
     3841 agttattcaa taccaggata aaccgtacgt tcgcctgaac ggcggcgact gggtgcctta
     3901 cccgcagtaa gcacgaaaaa ggccgcagag tttaacctct gcggcctgat ccgggcgcaa
     3961 attgccatta cggcagcctg acgcccgcat gacacgttac ttccgcttat tcagccagtt
     4021 cacgatcata ctggcaagaa tgccagcaat gaccggagcc gctaaatcat gccagaaggc
     4081 catgcccagc tctgcgagcg tcatatagcc gcctgtgttg taatgacaac gtttcgcggc
     4141 tattcttgag ttgtttgggt tcaagattag cccccgttat gttgtcaggt gcatacctgc
     4201 aacgtgcggg ggttttctct ctccagcaac caatgccacc agggataaag cccccgcaac
     4261 attgcgcctc accggaacct ttccggcttg ccgctgattc tacgactgtt tttctgttcc
     4321 gaaatccatc acttactcac tctgtgctgc gcattccgca gaatattatc cgcttgcaac
     4381 cgttgcattt ttcttacgaa aaaggccgca gagtttcacc cctgcggcct ggtccgggcg
     4441 caaattgcca ttacggcagc ctgacgcccg catgacacgt tacttccgct tgttcagcca
     4501 gttcacgatc atactggcaa gaatgccagc aatgaccgga gccgctaaat catgccagaa
     4561 ggccatgccc agttctgcga gcgtcatata gccgcctgtg ttgtaatgac aacgtttcgc
     4621 ggctattctt gagttgttca ggttcaagaa tggcccccgt gatgttgtca ggtgcatacc
     4681 tgcaacgtgc gggggttttc tctctccagc aaccaatgcc accagggata aagcccccgc
     4741 aacattgcgc ctcaccggaa cccttccggc ttgacgctga ttctacgact gtttttctgt
     4801 tccggaatcc gtcacgtctt cactctgtgc tgcgcattcc gcagaatatt atccgcctgc
     4861 aaccgttgca tttttcttac gaaaaaggcc gcagagtttc acccctgcgg cctgatccgg
     4921 gcgcaaattg ccattacggc agcctgacgc ccgcatggca cgttacttcc gcttgttcag
     4981 ccagttcacg atcatactgg caagaatacc agcaatgacc ggagccgcta aatcatgcca
     5041 gaaggccatg cccagttctg cgagcgtcat atagccgcct gtattgtaat gacaacgttt
     5101 cgcggctatt cttgtattgt ttggatgcaa gaatggcccc cgtggtgttg tcaggtgcat
     5161 acctgcaacg tgcgggggtt ttctctctcc agcaaccaat gccaccaggg attaagcccc
     5221 cgcaacattg cgcctcaccg gaatccttcc ggcttgacgc tgattctacg actgtttttc
     5281 tgttccggaa tccgtctcat cttcactctg tgctgcgcat tccgcagaat attatccgct
     5341 tgcaaccgtt gcatttttct tacgaaaaag gccgcagagt ttcactcctg cggcctggtc
     5401 cgggcgcaaa ttgccattac ggcagcctga cgcccacatg acacgctact tccgcttgtt
     5461 cagccagttc acgatcatac tggcaagaat gccagcaatg accggagccg ctaaatcatg
     5521 ccagaaggcc atgcccagct ctgcgagcgt catatagccg cctgtgttgt aatgacaacg
     5581 tttcgcggct attcttgagt ggtctaggtt caagattagc ccccgttatg ttgtcaggta
     5641 tatacctgca acgtgcgggg gttttctctc tccagcaacc aatgccacca gggataaagc
     5701 ccccgcaaca ttgcgcctca ccggaaccct tccggcttgc cgctgatttt acgattgctt
     5761 tttactgtca gcacccggca atcttcctca ttgcgcgatg ccttccagct taatttcttt
     5821 atttgcaata gttgcagttc atatccgggc tgactccaga ctggtagtga aatgcgcaat
     5881 tttgtagaat tgcgcctcct cgcgacctcc ccagtgaatc tgttgcctca ttttttacat
     5941 actgcgtatt cgacttctcc acctgttgcg caagagaaac tgggtttatt catttttgcg
     6001 agaccgactt ctttctggac aggactttat gcagcacaac acactatcga aacacaatca
     6061 gaaattgccg tttacacgct acgacttcgg ctgggtttta ttatgcatag gcatggcgat
     6121 tggtgccgga accgtgctga tgccagtaca aattggcttg aagggaattt gggtatttat
     6181 taccgcagcg atcattgctt atcctgccac ctgggtagtg caggacattt atttaaaaac
     6241 cctttctgaa agcgattcct gtaatgacta caccgatatt atcagtcatt acctggggaa
     6301 gaactgggga attttcctcg gggttatcta ctttttgatg attatccacg ggatttttat
     6361 ctactctctc tccgtggttt tcgacagcgc ctcgtacctg aaaaccttcg gtttaaccga
     6421 tgccgatctt tcacaatctc tactttataa agtcgctatt ttcgccgtac tggtggcgat
     6481 tgcgtctggt ggtgaacgat tactgtttaa gatttccggg ccaatggtgg tggtcaaagt
     6541 agggattatt gtcgtgttcg gttttgcgat gatcccgcac tggaatttcg ccaatataac
     6601 cgccttcccg caagcctccg tctttttccg cgatgtcctg cttacaattc cattttgctt
     6661 cttttctgcg gtatttattc aggtacttaa cccaatgaat attgcctatc gtaaacggga
     6721 agcagataaa gtactggcaa cccggctcgc gctgcgtacc caccgaatta gttatatcac
     6781 gctcatcgcg gtgatcctgt tttttgcctt ttcgtttacc ttctcaatta gccacgaaga
     6841 agccgtttct gcctttgaac aaaatatctc ggcactggcg ctggccgcgc aggtgatccc
     6901 tggacatatc attcatatca cctctacggt gctgaatatc tttgccgtac tgaccgcatt
     6961 ctttggcatt tatctcggtt tccacgaggc tattaaaggc attattctca atctgttaag
     7021 ccgaattatt gataccaaga aaattaactc acgcatgctg actctggcga tctgcgcttt
     7081 tatcgtcatt acattgacga tttgggtttc gtttcgtgta tcggtgctgg tgttctttca
     7141 gttgggaagc ccgttatatg gcattgtgtc gtgcctcatt ccgtttttcc tgatctataa
     7201 agtcgcacaa ctggaaaaac ttcgcggatt taaagcctgg ctaattctgc tttacggcat
     7261 tttgctatgc ttgtcgccac tgttgaagct gattgagtaa accggagcgc atggccccgg
     7321 ttttgtgagt taacgctgcg gattttcatc ctgatcaaca gcaaaacaag cgaccagttg
     7381 accgccgtag tcttttagct gcggctgcaa ctgggtgcag gggccgaagc gccgacgaca
     7441 gcgggcgttg aaggcgcaac ccggcggagg attcagcggg cttggcagtt caccggtgag
     7501 tttgatgcgt tcacgacgat cgtccgggtt caggcgtggc gtcgcggaga gcagcgcctg
     7561 ggtgtacgga tggcgcgggt tattgaagat ttggtccttc gttcctttct ccacgcagcg
     7621 gcccaggtac atcaccatca cttcatcagc aatgtgctcc accaccgaca ggtcgtggga
     7681 gataaagaca taagaaagcc ccaactcctg ctgcaaatcc atcatcagat tcagcacctg
     7741 cgcacgcact gagacgtcca gcgcagaaac cggttcatcg gcaatcacca cgtccgggtc
     7801 gagcatcaga ccacgggcaa tagcgatacg ctgacgttgt ccgccggaga acatatgcgg
     7861 atagcggtca tagtgctcgg ttttcaggcc gactttcgcc atcatcgaca gggctttttc
     7921 ccgacgctgt tctttgctga agctggtgtt aataagcagc ggctcttcga gaatttgccc
     7981 aactttttta cgcggattca gcgaaccgta agggttctgg aagacaatct ggattttctg
     8041 ccgacgcagc ttctgcgcct gcggatcgtg cttgagcaga tcctgcccct ggtaatacag
     8101 ctcaccaccg gtgggcgttt cgatcatcgt cagcaaccga ccgagggtcg atttaccgca
     8161 gccagattcg cccactactg ccagcgtttt gccacgttca aggttaaacg aaacgccgtc
     8221 cagcgccttc accagacgtt ccggcgcgaa catgcctttc ttcaccggat aatgtttttt
     8281 caggtcgata gcctgcaaca gcggttgttg cgaggtggcc tcttgcgtac tcatagtgtc
     8341 ggcctcccgg catcatcaag tgggtaatgg catttggact gacgcccatc agcgagcata
     8401 ttcagcgccg gttcttcagc gcgacatctg tcagtggcat aggggcagcg cgggttaagc
     8461 aggcagccgt tcgggcggtc gtacttaccg ggaacgacac ctggcaacga cgccagacgt
     8521 tctttgtcct gagcaaattc tggcagcgca cgcagcaatg cctgagtata cgggtgacgc
     8581 ggcgcatgga agatggcgtg cgcatcaccg gtttccacca cctggcctgc atacatcacg
     8641 atgattttat gtgccgcttc cgccaccagc gccaggtcat gggtaattaa caccagcgcc
     8701 atgttctctt tctgctgtag ctccagcagt agttcgatga tttgcgcctg aatggtcacg
     8761 tccagcgcgg tggtcggttc atcggcaatc agcagttttg gccgacaggc aatcgccatg
     8821 gcgatcatca cgcgctggct catgccgccg gaaagctgat gcgggtaaat atccagacgc
     8881 gatgccggat cgggaatacc gacctgattc agcaggtcaa tcgcccgctg acggcgagtg
     8941 ctcttattac cgccctgatg caccttaatc gcttccatga tctggaaacc cacggtgtag
     9001 cacgggttaa ggctggtcat cgggtcctgg aagatcatcg ccacttcggc acccaccagg
     9061 ttgcggcgct ctttttctga tatacgctgc aaatcctgac cgttaaacac cagcttttcc
     9121 gccattacgc ggcccggata atcaatcagc cccataatcg ccagtgaact gaccgactta
     9181 ccggaaccag actcacccac aatcccgacc acttcgccct gttttacgct gtagctgatg
     9241 cggtctacgg cgcggaacgg tgcgctttcg tcgccgaaat gcaccgataa tttatctaca
     9301 tttaataacg ccatctcgaa cctcttactg cttcagtttg ggatcgagcg cgtcacgcag
     9361 accgtcaccc atcaggttaa atgccagcac cgtcagcagg atcgccagac ccgggaaggt
     9421 cacgacccac caggcgcttt gcgcgaactg caacacgtcg gagagcatgg tgccccactc
     9481 tggtgttggc ggctgcgctc ccatgccgag gaagccaagg gcggccatat cgagaatggc
     9541 gttagagaaa ccgagcgacg cctgaacaat cagcggcgca aggcagttcg ggaagatgtt
     9601 aataaacatc tgacgcatcg ccccggcacc cgccacgcga gacgcggtga cgtaatcgcg
     9661 gttaacttcc accagcacgg cggcgcgggt taagcgcaca tagtgcggca aggcaacgaa
     9721 ggtcagtgcc agcgcggcgt taccaatcga cgggccgaag attgccacca gcaccagcgc
     9781 cagcagcaga cttggcagcg ccagcatgat atcgaccacg cgcataatga tgttatcgac
     9841 caggccgcca aagtaaccgg cgatcaggcc gagaataacg cccataatca gcgataacac
     9901 aactaccaga cagccaacca gcagcgacag gcgcgcaccg tacatcaggc gcgacagcac
     9961 atcacggcct acgtcatcgg tgcccagcaa gtgcgccatg ctgccgcctt cctgccaggc
    10021 tggcggggcg agcagtgcat cgcggaactg ttccgccggg ttatagggtg caatccagtt
    10081 ggcaaagatc gcgatgaaca gcacgatgac gacgtaaacc agcccgacga ccgcgccttt
    10141 gttgcgttta aaatagtgcc agaactcctg taacggggtc atcggcaccg gtgcgctaat
    10201 cactttattt tcagtaacct gtgacatgat gtccccttac ttcttatgac gaatacgcgg
    10261 gttcaccacg ccgtacagca gatcgaccag caagttgacg aggataatca tcgtcgccac
    10321 cagcaatacg ccgccctgca ctaccggata gtcgcggcgt tgcagcgcgt caatcaacca
    10381 gcgccccaga ccgggccacg agaagatggt ttcggtcaga atcgcccccg ccagcaatgt
    10441 tcccacctgc aggccgataa cggtcactac cggcagcatc gcgttacgca gcgcatggac
    10501 gataatcacc cgcatgcggg ttagcccttt ggcgcgcgcg gtgcggatgt aatcctcgcc
    10561 cagcacttcc agcatcgagg agcgtgtcat acgcacaatg accgccagcg gaatagtacc
    10621 cagcacaatg gcaggcaaga tcatatgggc gacggcatcg ataaagttgc cgtcttcacc
    10681 ccagatggcg gtgtcgatta gcataaaacc ggttaacgga ttggagtcat cgaggaacac
    10741 catatcgctc acgcgaccgg agacgggcgt caggttccag tgcaccgaaa ccagcatgat
    10801 cagcatcatg ccccaccaga agatcggcat cgagtagccg gtcagcgcca ggccaaccgc
    10861 tgtgtgatcg aaaatggaac cgcgtttaac cgcagccagc acgccgaccg gaataccgac
    10921 cgcagtcgca aaaatcatcg cgcagacgcc aagttccagc gtggcctgga ggcgcggcac
    10981 gaactcttcc caaaccggga tgcggctttt cattgaaatg cctagatcgc catgcataac
    11041 gccccaaatg taatggagat actgctgcca catcggttta tctaagccga gttcagccag
    11101 cagctgcgcg tgacgctctg gggagatccc acgttcgccc gccatgatca tcaccggatc
    11161 gcccgggatc atgtggacaa aggcaaatgt gagaagggta ataccgataa acgtggggat
    11221 gacgagtccc aaacgtcgga gaataaactg caacataacc cggattctct atagtgaccc
    11281 gcagcctgga actgcgtgtc tgtattgctc acaaatcaat tcccgcctag gtcgggctgg
    11341 gaatgaagta ttgctgccgg atggcgctgc acgcagaggc acagcgccac cgggtatggc
    11401 ttttaattat tcgatagaga cgttttcgaa gtgatgtttg cctaatggat caaccacata
    11461 gcctttaact tctttgcgta ccggttcaaa cacggtggag tgagcgatga tcagtgccgg
    11521 agcctgatcg tgcatcacca cctgcgcttg cttgtacagt tcaacgcgtt tgttatggtc
    11581 gtcggtagca cgcgccggtt gaatcagatc ttcaaacggt ttgtagcacc attttgagta
    11641 gttggagcct tgttcagagg cggcgcagct gaacagggtg gcgaagaagt tatccggatc
    11701 cccgttatcg ccagtccagc ccatcattac cgtctggtgc tcgccatctt tcgcacgctt
    11761 gaggtactca ccccattcgt aggtgacaat tttggcctgc acgccgactt tcgcccagtc
    11821 agcctgaatc atctccgcca tgcggcgagc gttcgggtta tatgggcgct gtaccggcat
    11881 cgcccacagg tcgatggaga aacctttttc cagacccgct tctttcagca aggctttcgc
    11941 tttttcagga tcgtaggtgt aatcctgaac gtcgtcgtta tagccccaca tggttggcgg
    12001 gatcaggttt ttcgctgata cacccgcgcc ctgataaacc gctttgatga tcgcgtcttt
    12061 gttcaccgcg taggtcagtg cctggcgaac tttcacgtca tccagcggtt ttttctgcac
    12121 gttataggag aggtagccga cgttcagccc cggcatttcc atcaggttga tggatttatc
    12181 ctgcttcatg cgagcgatgt ctgccgggtt cgggtacggc atcacctggc attcattctt
    12241 ctgcaatttc gcataacgca cggaagcgtc aggagtaata gagaagacca gcgtatcgat
    12301 ctgcggtttg gtgccccagt agccatcaaa cgctttgtag cggatacggg agtctttttg
    12361 gtactgctgt aactggaacg gaccggtgcc gattgggttg aggtccagtt tttccggtgt
    12421 accggctttc atcatcgcgt cagcatattc ttttgacaga atagaggcga agtccattgc
    12481 caggtcagcg aggaacggcg cttccgggcg agtcagcaca aactgaacgg tgttgtcgtc
    12541 cacctttttc acttcgctga tcagctccgg caagcccatg ccttcgaagt attcgtagct
    12601 gccgccagaa actttatggt acgggttttg cgcgtttttc tgacgatcaa acgagaacac
    12661 cacgtcatcg gcgttcagtt cacgcgtcgg tttgaattct ttattgtcgt gccacttcac
    12721 acctttacgc agatggaagg tataggtttt accgtcttcg ctgacttccc acttttcagc
    12781 gaggcccggg atcacttcag tggtgccgat tttaaattca accaggcggt tataaagcgg
    12841 tacggaagag gcgtcatagg tggtgccgga ggtaaacagc tgcgggttaa acccttccgg
    12901 agatccttct gagcaataaa ccagagtttt agcctgaaca cttgctgcga cggtcatagc
    12961 caccaggctg agaccaagct tcagcatccc tgactttttc aaggaaatac gcattattct
    13021 gctccaattg tgatgtttgt tgttttaacc ctttgcagtg ggttgtcgct gcctgacctt
    13081 ttttgttttt tgcccggtcg ggtcaacgtt atgaggtggg gatgccgtac taattaacat
    13141 cagtgtaaca gtgcggatcc tccataaaat gcccctcgtg acctacaatc tgtcaacaga
    13201 atgtgaaaac gtcaatacag ccaaccggga tttacaccaa cggtgagaat ccacaaacaa
    13261 agattaaaaa aacttcaaac agctatttgc agcagagaaa tttgtgctac tccaacatga
    13321 ccagaacaat cagcttaata tttagcaaca tttggtgatt aagttttatg catttaatga
    13381 aaaaaatctg aggaaaaggt ggatatctga gcgattaata ccatgaacgc taaaacgcac
    13441 agcggaaaat gccagcgcca gccataagta acgcaaaaaa agatttaagc aaatataaaa
    13501 aaagacaatg gtttctgtca caaatccgtt aatggatagt gagatatggg gcgcaaagtt
    13561 ggcaggaaaa tgtgtggtgt gttggtcgaa taaggcggtc gcgccgcatc cgacaataag
    13621 tgcggtgccg ttgcctgatg cgacgctg
//
LOCUS       DAZFJF010000113        13606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFJF010000113 DAZFJF010000000
VERSION     DAZFJF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.113"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..912)
                     /locus_tag="ACHKAU_003799"
     CDS             complement(25..912)
                     /locus_tag="ACHKAU_003799"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5532780.1"
                     /translation="MASNYGENFTPLFKRFIKYIFKNPIAKTTTNKERFHLNLLEMTI
                     YGEMASFNAAGCEIFISEQRLADDYDVSRPTVINSIKSLENAGLISIVKSKGKSNHYK
                     LLITPDQLGKMCDEQGIKTSDSVGSSKEAPDNQQEAPKQATQQAKKAEVVQLPTQDTK
                     APQINAVSDKPRRGRPVKDVTDGGWIVLGESEAKERMRSLWESKYKGLNKRCPIRLSK
                     IQEDFYTTEEVIRHKGTRDGEIWKPEEYWHLTRPVFIFANKLIGMGFEILLDQTYGDY
                     PEILWRNVRKNKKGILEFR"
     gene            complement(926..1141)
                     /locus_tag="ACHKAU_003800"
     CDS             complement(926..1141)
                     /locus_tag="ACHKAU_003800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532781.1"
                     /translation="MAKTGPISQLVNWTTDEIVENVLKGNFRKTSVYAFIRKHKGEYP
                     HIEEAILKLNCIRLERELAKYTNNINK"
     gene            complement(1275..1424)
                     /locus_tag="ACHKAU_003801"
     CDS             complement(1275..1424)
                     /locus_tag="ACHKAU_003801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532782.1"
                     /translation="MAISDAIAIINLAVLWCGVAYIAGVTAIDNLEEKMIDKYKKIVD
                     NLTNV"
     gene            complement(3740..4327)
                     /locus_tag="ACHKAU_003802"
     CDS             complement(3740..4327)
                     /locus_tag="ACHKAU_003802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532783.1"
                     /translation="MNIKTIIAALTIAFSANTAAIDVGAVKDASCADKLAALDAGFDA
                     PQGYQMGLSLKNIILPMESGAYVYGAIGKLEKPEIDLQGVRAKITKRNESLCDVAEIA
                     FMSDDEKSILAFCKDSDDNDRFFKITHNRPISFYSNATMGAGMQWLKRAKVTEIDIEK
                     EANAGSYGFWKGWTKYTFQQCGIDSQAYDNGFWFF"
     gene            complement(4385..4645)
                     /locus_tag="ACHKAU_003803"
     CDS             complement(4385..4645)
                     /locus_tag="ACHKAU_003803"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5532784.1"
                     /translation="MTGAKDIRRLREELIAEIKTKAKQMEIAAQMDVAEQMYKMVEEG
                     TPRQVIAKTFGVGIATVHRRLKAYADYHNIERKEDGRFRKAK"
     gene            5663..5998
                     /locus_tag="ACHKAU_003804"
     CDS             5663..5998
                     /locus_tag="ACHKAU_003804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532785.1"
                     /translation="MRDMTIRTRGHIISYAADEQFSIRDVEVKNKVKAEIPAGTVVVV
                     TEAGEVETFVKATHTGKTPLLVVERAEVGDAFIVVANKGCYINPAYVDKEGLAAVVAA
                     SNGDLRVAQ"
     gene            6010..7044
                     /locus_tag="ACHKAU_003805"
     CDS             6010..7044
                     /locus_tag="ACHKAU_003805"
                     /inference="COORDINATES: protein motif:HMM:NF015802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5532786.1"
                     /translation="MAKKEFEVRDLCGLIKDIPPDNMIISAMDIFNTTALCNTFKFEY
                     DKVRQVKQTAFGQYPRYGEQMATITADERETGTGDMAFTAASSAIYPSDWQGIRVPGK
                     DSEENLESVTARRVIQLAEGLRQTHEEARQLALFENKIILTKGKELNVCELLGVEQST
                     SEVDLAQDILKQLQDLVALSKVKQRGVVRALRGFTLFCRAELFTAIQTDELLRKVLLF
                     GNQNITDKGLMFPEDILPGFPTFRIPGLALRVVQMDSLPENTAYLVPQFQPHALGDMV
                     GVYTQFFGPCARNLKLANGAAREAFMYLEESRGKREIFVESSYAFMNMMPEVVVKVSV
                     KNTAAAAKAK"
     gene            7186..7437
                     /locus_tag="ACHKAU_003806"
     CDS             7186..7437
                     /locus_tag="ACHKAU_003806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532787.1"
                     /translation="MKIVLYGDRDDVPFDIIHIDDEDLPEFLRISTFAACYDVSRQAM
                     HKYREYNKCTWAETFEHFIARKQAQDRKKALNAHNRFAK"
     gene            7601..8404
                     /locus_tag="ACHKAU_003807"
     CDS             7601..8404
                     /locus_tag="ACHKAU_003807"
                     /inference="COORDINATES: protein motif:HMM:NF044880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFO5532788.1"
                     /translation="MASDALTAAKQAESRSPAFTVMCGGYSQNKNIMDGFNYTDNVAI
                     FFDSISDVSYSFPVEKSSYAVEDGTTMSDHAVIKDKSVSFTGIITSSPHIIRKENYID
                     RNTDPDNPRESMRPAAAVQALMEMRDKRQLVSLVSEDAGLLENFIMTKCEVKRSSSSG
                     DCITIDCTFEEFRTFKVKMVENAGVFTNSKKVNKTQQKGAVNSAKEGSNVGNSASKPK
                     AQHRGDISKKFADGYELGGGEKILSNEAHASMVDKANMYVDDLSGAGIL"
     gene            8401..8865
                     /locus_tag="ACHKAU_003808"
     CDS             8401..8865
                     /locus_tag="ACHKAU_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016068527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532789.1"
                     /translation="MSPKVRCTIDKRMMDKLHKELSQMEGVAVGWDDGKHYSGLNYAT
                     LAAIHSEGWGDLPKRSFMESAHAAMLYESLRHTKALINAIVKGGSSEAVRKRIGKRYA
                     DILETILISGQFSNNKVSPQWASYKGFNDAMRHYDDLIGAIKYWVDIDKKGA"
     gene            complement(8884..9033)
                     /locus_tag="ACHKAU_003809"
     CDS             complement(8884..9033)
                     /locus_tag="ACHKAU_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532790.1"
                     /translation="MKNANAIKRNNRKIHARKFLKTEEGRKWLEEKQKERQEMKLVRE
                     LSQFI"
     gene            complement(9543..11663)
                     /locus_tag="ACHKAU_003810"
     CDS             complement(9543..11663)
                     /locus_tag="ACHKAU_003810"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5532791.1"
                     /translation="MAMKEVARLTNVIYWDSDKSSWRKEVKRLEDYKKSWKKATDDIA
                     KADFKSNQAVIRTQRTRAKVEEQIARAQRKQQEAQKKQRAQAVSRLTGGNNPELSSMR
                     KFYQQQAREAARQQKLLDRSVGDVQHRKARVGANSARIGSMFDGSLVAQFQGRIQRVN
                     ADYAKGAINLQTYNQKMARLRAEMKMATAEAGKFQSRLDKLASGASKVHDNLGKINST
                     LGKIAIGLTAAGWGVQRAVSGAMGSLADRNSAYFGLMAQNKDDKAKTDQQMAFIKNTA
                     YTYGMDEREMQGGYMRFAASTPTMKESDKQALFEAMAIKGRSVGATGEQQNRAIVAIS
                     QMAGKNRVMAEELRGQLSEAFAGSQQDFQQAAIDAGIIKNAQEFDKAMKDGKITLDKL
                     LPSLVKIWGAAKETKAFAESMKQPEVALQRMKNSFNELAIQFMGVVDPADGVVTLSEH
                     LISIFEDLTYVMGDGGSTATQMGSQIGAAIKGFVEWMAFAAGFITARTEQFSKNGTEV
                     AEIIGKLTALMISLKVVGSLLTPFTGLISLVTQLIALLGKIPGMSKLLASASGGTATG
                     AAVISAGSNAERSFIDGKTQEMSKENSKQGALFELGRFVSNAGALFTPGGDTGPLFDQ
                     VRGWFNKGPTLTVDTGIRPTPTGGIAIQNGGYQPVQVNVAPQGEISGKWEITIDEGGI
                     ARVIQKEVSDQLDWKATQESNQLF"
     gene            complement(11667..11903)
                     /locus_tag="ACHKAU_003811"
     CDS             complement(11667..11903)
                     /locus_tag="ACHKAU_003811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532792.1"
                     /translation="MNIYEKIYQKLVKDSEDTPEGVTIGHLPEAAKEGATIIKSGPHT
                     EGLSIEELAEKYNIDKEALKRAISLSKSLRQGEV"
     gene            complement(11905..12288)
                     /locus_tag="ACHKAU_003812"
     CDS             complement(11905..12288)
                     /locus_tag="ACHKAU_003812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532793.1"
                     /translation="MNIEEMIVEFEEGDFLEDGEFKYSRFKQALFSSDDKPHVSDLND
                     DDLCRWVCTRLYEDGKYTGQNFPNLYFKAQTFEEGQRVKNLIDKMLSAGYLHKELLNP
                     KAESKRHYIFVWTIGDKCPLRRKGK"
     gene            complement(12349..12792)
                     /locus_tag="ACHKAU_003813"
     CDS             complement(12349..12792)
                     /locus_tag="ACHKAU_003813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532794.1"
                     /translation="MSQIAKLLSQVIKAIDAEDSKVRQDLLDGKFTNTLAFTRDKEHF
                     LKHRHHAFAECKSANGDVFYVDLVVMACRLWMIQDLMRQHGDDPLISMWNSAQDGFVI
                     AALSKSMIEAEGASLQNMADLFEISVGMMQTMLQAVKPLKLVNLD"
     gene            complement(12866..13354)
                     /locus_tag="ACHKAU_003814"
     CDS             complement(12866..13354)
                     /locus_tag="ACHKAU_003814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532795.1"
                     /translation="MFKSDLSDSQFEKLVALQKYLPSDDQPTRQAKHLLVEGFTPEFV
                     AEYTGVKLEKVQKLYNESWNPRCRVPLSVYNEIDRDRNVFEDRRRKSLLMWNCGMNIE
                     EIAKELKVTCFHIWGLLSEILPRKELNQRLPKPGSRAYREFIFIYRRHRNKVQKAMAQ
                     KA"
BASE COUNT         3579 a         3056 c         2917 g         4054 t
ORIGIN      
        1 aaaaaaataa gattatcaat atttttatct aaactctaaa atgccttttt tattctttct
       61 aacatttctc cacaatattt caggataatc accgtaagtt tgatctaaca atatttcaaa
      121 tcccatccca ataagtttat ttgcaaaaat aaaaacaggt ctggttaaat gccagtattc
      181 ttccggtttc cagatttcac cgtctctagt acctttatgg cgtattacct cttctgtggt
      241 gtaaaaatcc tcctgaatct ttgacaaacg gatcgggcaa cgtttattta aacctttgta
      301 tttgctttcc cacagtgagc gcattctctc tttagcttcg ctctcgccca ggacaatcca
      361 gcctccatcg gtgacatctt tcacaggacg gccccggcgt ggtttgtcac taacagcgtt
      421 aatttgaggc gctttagtgt cttgggtagg caattgcacc acctcggcct ttttagcttg
      481 ctgtgtggct tgttttgggg cttcctgttg attatcgggg gcctctttac tagatcccac
      541 actgtcggag gttttaatac cctgctcatc acacatcttg ccaagctgat caggggtaat
      601 tagcagtttg taatgattag atttgccttt acttttaact attgaaataa gcccggcatt
      661 ttctagagat ttaatcgagt taataactgt cggtcttgaa acatcataat cgtctgctaa
      721 tctctgttca gagataaata tttcacaacc tgcagcatta aaacttgcca tctcaccgta
      781 aattgtcatt tccaacaagt ttaagtgaaa tctttcttta ttggttgtag tttttgcaat
      841 cggattttta aaaatgtatt tgatgaaacg tttaaaaagg ggagtaaaat tttccccgta
      901 attacttgcc ataattcccc cataattatt tattaatgtt attggtgtat ttagccagtt
      961 cccgctcaag gcgaatgcaa tttagtttta aaattgcttc ttcaatgtgt ggatattctc
     1021 ctttatgttt acgaatgaat gcataaacgg aagttttgcg gaagttcccc ttaagtacgt
     1081 tctcgacgat ttcgtcagta gtccagttta ccagctggct gatcggtccg gttttagcca
     1141 tagtataagc ctcctgttag ctcactaacc tgtgcgatat atgattaaaa agaaacggga
     1201 actcaagaca agctcccgca tttttgttta aggaaattca ctatgatgag aattacccta
     1261 tgctttatat tttatcagac attcgtcaaa ttgtcaacaa tctttttgta tttatctatc
     1321 attttttctt caagattgtc gattgcagtt actccggcta tgtaggcaac accgcaccac
     1381 agtacagcca ggttgatgat cgctattgca tcactgatcg ccatattgac ctccagatct
     1441 gtttccccac catgtatata atcttatcat actgtcaaca gaatgtcaat aggaatcatt
     1501 atcatttagg cttgccatat gtgcaagcaa gtccacagca aaggggtaaa cattattaat
     1561 ggcccctacc gtgtctaaac caaaattttt tatagcattt atgcaataag attgtcaatg
     1621 gtccatactg gagggagagc actttttgtt aaaactttgg agggtgttaa actcaaacct
     1681 catgagaggc cctacaacgc gatttaagca tcaggcaatg cgattgcact actcacacag
     1741 gttaatgcag ccagaaggcc atttaggggc cttattttcg atttatgagt gtatccttta
     1801 tatatgtgtg cgtgcacccg cgcgagaaac cactcgctta aacggtccgg attggaaaca
     1861 gaagtctctt atccggactc cacattatat agctacgtgc tatcgcacta cgctccccac
     1921 acagaggccc gaagggttaa gccagaggct taagtgctgt aagcacccca cacagattgc
     1981 ctaaagagtc gctaatgctc tttaggcttt catttgtggg ttaagctttt cccttagaaa
     2041 accttcccca ctctctgatc cctccccatt ccccttgggg gggttagggg ggatctattg
     2101 tgtggtctat tactttatct attagtgtca aatattttta caggtagtag taaaagattt
     2161 tgacaggtag tagtaaaaat attttacagg tatgggccgt attattggtg gggtagtaaa
     2221 atattttgac aggttagggg ctgtgtatca tgggtgcaat gactgcaaaa caggagagga
     2281 tgactgtata aacacacagg tgtgaaaatt ctttacaggt tagtgcggta atatttaccg
     2341 gggtgtggtg cctgagtatg actgaaaggg gttgtaggaa atcccaacag gtgggggagt
     2401 gattgcatct gattttttac tctgtattag ccgtcaattg acctattttg actagcaaac
     2461 ctctctcctc tctctttgct ctgattttct ctcatgtgtg gtaaatatgc agatcaacac
     2521 ccgttgattt tatcaatagc tatgttgacc gggcatcata agtgtggccc ctgaggcagt
     2581 ccagccttct ctcacacagg acaatgaata cattccttgc agtcatgaat acataaagaa
     2641 catgaaagac tgttcacacc aacacgccga taaattgcct aaagaggctt gcatggggtt
     2701 attgttgtgt gggtaatggt gatcatgcta acagattatg ttaatcagat gtaaattcca
     2761 attttgtggt ctgcagggaa caaactgtac tggttaaatg atcaggggaa tgactacaaa
     2821 acaatcaaaa atgtgggata tagacatcta cacgtctaat gtgctgtgtg gggtgttagt
     2881 gcatgcttac atgtaagtca ttgatttgaa gggttgggaa aattgcattt aacataatag
     2941 atcttatcta cactaagaaa ccgaactaca aaatctgatt taaaaatcat gatcaggggg
     3001 cccagaattt acgtacatgc acctgaataa ataatgtgca caacatacag caaagcttaa
     3061 gaattaacat aatagcaacg tggtgggcag tggtgtgata tttgagaatt gttagaagaa
     3121 aaaagggagg tgggggagcg cctgcctggc acctcagacg aacatctgac gggtattaat
     3181 accgctaatg gatatttctg acacccacac ccaagaccgc tttcagcaca cagccatata
     3241 ccgcacagga tgcaattgtg ataacaaaaa atgggggtgt aatagggtag gtcatctttc
     3301 atcaaaatca tctctacgac gtttctagag tgataacaag aaaacaggcc tctaagatta
     3361 tctgtacctc gtttttagct ttttgtgtgg ttaccaggtg aagaggaaat gttaaaaact
     3421 gttcatttta tgcacaaact agtcagggtg ataccacaaa ttgaggtgta agaaatccag
     3481 ctgcagataa tcttgcagcc agattttgca gtttttgagg gtatgttaaa tttgacagaa
     3541 taagagaaga ggctaattgc gctaagttat tgattttact cattatctaa aaatgggtga
     3601 tcaccttact cttacactgt caacaatgcc taaaaccatt aaatcaataa ataggcatta
     3661 aatacccatt aaataggggt taaatagggg ataaataaaa aggggccgtg atggcccctg
     3721 gcgggattaa cttagtaggt tagaaaaacc aaaatccgtt gtcgtaggcc tgactatcga
     3781 tgccgcattg ttggaacgtg tatttagtcc agcctttcca gaatccataa ctgcctgcat
     3841 tggcctcttt ttcaatgtcg atctcagtta ctttagctct cttcagccac tgcattcccg
     3901 cgcccatagt agcattagag tagaaagaga ttggcctgtt gtgggtgatc ttaaagaagc
     3961 gatcattatc atcgctatct ttgcaaaagg ctaaaataga tttttcatcg tctgacatga
     4021 aagctatttc agctacatca caaaggcttt catttctttt agtgatcttc gctctcacac
     4081 cctgcaggtc aatttccggc ttctccagtt ttcctatcgc tccgtataca taagcgccag
     4141 attccatggg gagaataata tttttaagag atagccccat ttgatagccc tggggtgcat
     4201 cgaatcccgc atcaagtgcc gccagtttat cagcacaaga tgcatctttt acggccccta
     4261 catcaatagc tgcggtgttg gcactaaagg caatggtgag ggctgcaatg atagttttaa
     4321 tgttcatagt ggtacccctt tgcgagaagt tgaattaagt tagtaacctg ttggaccgtt
     4381 tacatcattt agctttcctg aatctcccat cctcctttct ctcgatattg tggtagtcag
     4441 catatgcttt cagccttctg tgcacggtgg cgatccccac accgaaggtt ttagcgataa
     4501 cctgtcgtgg cgttccctcc tcaaccatct tgtacatctg ttccgctaca tccatttgtg
     4561 cggctatctc catttgcttt gccttagttt taatttcagc tatcagctct tccctcagtc
     4621 tcctaatatc tttagcccct gtcataaact cctctcaatg ctgttcataa actcatctca
     4681 atgatgatta atacaaatct aagactgcca gacttattaa aaatgctttg cctcctttgc
     4741 ccatctcaca cctccacaca cagagctaac atcagggcta aaactccaac cacctcaatt
     4801 gccttaataa tgtgtgggtg atgaaacctg gccttttgcc atgcgctggc actgttcttg
     4861 atacccataa ctatcacctt ttgcgagaag ttgatttgtt tgttcggttg ggtgctttca
     4921 ctggctatgc caggtgactt tcagtcaccc ctttgggtgt ctctatcaat gctccccttc
     4981 cttgattcga actatagccg ggcaaatccc cggaaaacat tgatattgat caaaatatat
     5041 ctatcacgaa tgaaaaagtt atgatagatg cgatgagatg acgtattgat gggattgata
     5101 gcagtctgaa aataccgtta ctacgctaat tattagaatt tatataatca gggtgttgac
     5161 aatttgagag aatactgata taatatacat ataccgtaaa tcaatggtga aagtgatttg
     5221 ccgtattttc ctccaaagta aaggcgacca ggtagcggtg ccgccttata aataacacta
     5281 ccctgatcat cgttgagtgg tcgattaatt ctcctattgg gctagttatc cacaccgttt
     5341 tcttttgcgg gttttctgtg tgggtagcta tcccgaaagg tatttccccg ctgatgtgac
     5401 cgcagcctga actgattcgg gggagtgcct ttcgtgatag tgctctctag taaccgtaac
     5461 ccatactgtg aactatcatc cgactgttgt cggtttcccc atgtggaata gcccccgctt
     5521 ggacggtgcg gggctatctc cctctcctta tatcaatccc cctttagatt tccggcataa
     5581 cttttcggcc cgagcctgtc gtggtttgag aggttatacc tgagcccttt gaggggagtt
     5641 aatttaaatc gcaggtgatc aaatgagaga tatgactatc cgcacacgcg gacacatcat
     5701 ttcatacgcc gcagatgagc aatttagtat tcgcgacgtt gaagttaaaa acaaagttaa
     5761 ggctgagatt ccggcaggca ctgttgttgt ggtgactgag gctggcgagg ttgagacctt
     5821 cgttaaagca acgcacaccg gtaaaactcc gctgctggtt gtagaacgtg ctgaggttgg
     5881 tgacgctttt atcgttgttg cgaacaaagg ttgctacatc aatccggcct acgtggataa
     5941 agaaggctta gccgctgttg ttgctgccag caatggcgat ttgcgtgttg cacaataagg
     6001 aggttgaaaa tggctaagaa agaattcgaa gttcgcgatc tgtgtggtct gattaaagat
     6061 atcccgccgg ataacatgat tatttctgca atggatatct tcaacactac tgctctgtgc
     6121 aataccttta aatttgaata tgacaaagtt cgccaggtga aacaaaccgc tttcggtcag
     6181 tatccgcgct acggtgaaca gatggcgacc attaccgcag atgagcgtga aacgggcacg
     6241 ggtgatatgg cgttcactgc cgcatcaagt gctatttatc cttctgactg gcagggtatc
     6301 cgcgttcctg gcaaagattc cgaagagaat ttagagtctg taaccgctcg tcgtgttatt
     6361 cagcttgccg aaggcttacg ccagacccac gaagaagcac gccagcttgc tctctttgag
     6421 aacaaaatta tcctcaccaa gggtaaagaa ctgaatgtgt gcgaactgct gggcgtagaa
     6481 caatccacct ctgaagtgga tctagctcaa gatatcctta aacagctgca ggacctggtt
     6541 gcactgagca aggttaaaca gcgcggtgtc gttcgtgccc tgcgtggttt caccctgttc
     6601 tgccgtgctg aactgtttac cgctattcag actgatgaac tgctccgtaa agtgctgttg
     6661 ttcggcaatc agaacattac cgacaaaggt ctgatgttcc ctgaggatat tctgccgggc
     6721 ttcccgacct tccgcattcc gggcttagcc ctgcgtgtgg tgcaaatgga tagtctgcca
     6781 gagaatactg cttatctagt cccacagttc cagccgcatg cgttaggcga tatggtgggc
     6841 gtttatactc aatttttcgg cccttgcgct cgcaacctga agcttgcaaa cggcgctgca
     6901 cgtgaagctt tcatgtatct tgaagagtca agaggtaagc gcgaaatctt cgttgaatcc
     6961 tcttatgctt tcatgaacat gatgcctgaa gttgtggtta aagtgagtgt taaaaacacc
     7021 gccgccgctg ccaaggctaa ataaaagcaa agggaagagg tgagagatcg gggataggtc
     7081 tttccctctt cccttttttg ttatccacag acccaaaggg cttagcttta gctaaggtcc
     7141 tggaaggact cataatcccc gtcttaagtt tttgaggtgt cttttatgaa aattgtttta
     7201 tacggcgacc gtgacgacgt gccttttgat attatccata tcgatgacga ggatctgccg
     7261 gaattcctgc gtatctccac atttgccgcc tgctatgacg ttagccgcca ggctatgcat
     7321 aaataccgtg aatataataa atgcacctgg gccgagactt tcgaacattt tattgcacgc
     7381 aaacaagccc aggaccgcaa aaaagccctg aatgcgcata atcgttttgc aaaataacga
     7441 tagatagccc tgtcttttta tcagaattca cgcagggcaa cgataagttc tattttgcct
     7501 gcatcttttg caggtttggc tttaacaccc ttgagacgag ggctatttgc acgagtttag
     7561 ccaaatccac gaagtggacc aatttggagg catgtttaat atggctagtg atgctctgac
     7621 agctgcaaaa caagctgagt cgagatctcc tgcattcact gttatgtgtg ggggatattc
     7681 acaaaacaaa aatataatgg atggcttcaa ctataccgat aacgtggcta tcttctttga
     7741 ctcaatctct gatgtcagct attcgttccc tgtcgaaaag tcgagctatg cagtagaaga
     7801 tggcaccacc atgtcagacc atgccgtcat taaagataaa tctgtcagct ttacgggtat
     7861 tattacctct tcccctcaca ttatccggaa agagaactac atcgaccgga acactgatcc
     7921 agataatccc cgtgaatcta tgcgccctgc tgccgctgtc caggcactta tggaaatgag
     7981 agacaagcgc cagctggtta gcctggttag tgaagatgcc ggacttcttg agaacttcat
     8041 catgacgaaa tgtgaagtta agcgtagctc ttcctctggt gactgcatca ctattgattg
     8101 cacttttgaa gaattccgca ccttcaaggt gaagatggtc gaaaatgccg gagttttcac
     8161 taacagtaag aaggtgaata aaacacagca aaagggtgct gtgaatagtg ctaaagaggg
     8221 tagcaatgta gggaactctg caagcaaacc taaagcacaa caccggggag atatttctaa
     8281 aaaattcgca gacggttatg aacttggtgg cggggagaaa atcctgagca atgaagctca
     8341 tgcgtctatg gtggacaaag ctaatatgta tgttgatgac ctgtcaggtg cggggatctt
     8401 atgagtccta aagtaagatg caccatagac aagcgcatga tggataagct gcataaagag
     8461 ctctcacaga tggaaggtgt agcggtaggt tgggatgacg gaaagcacta tagcggcctg
     8521 aattacgcca cactggcggc tatccactct gaggggtggg gtgatttgcc taagcgctcc
     8581 tttatggaat ctgcccacgc tgcaatgctc tatgagagcc tgagacacac aaaagccctg
     8641 attaatgcaa tcgtaaaggg tgggagctct gaggccgtca ggaagcgaat tggcaagcga
     8701 tacgccgaca ttctggagac tatcctgatt agtgggcaat tcagcaataa caaggtttcc
     8761 ccacaatggg caagctataa aggcttcaat gatgcaatgc gccactatga tgatttgatc
     8821 ggtgcaatca aatactgggt agacatagac aagaaagggg cttgatagcc cctctccctg
     8881 agattaaata aactgggata attcccgcac aagcttcatt tcctgccgtt ccttctgctt
     8941 ctcttccaac catttccggc cttcttcagt cttgagaaac ttgcgagcgt gaatcttgcg
     9001 gttatttcgc ttgatggcat tggcattttt catagtgtca cccctcttat gacctgcgac
     9061 gcctacatta tagccaaaat ccggaattca gacaatagaa ctctttttga tgccgtgttg
     9121 gtggtaagaa gaaacactag catcatttaa aggttttagg ttttcatgta gatcagcttt
     9181 atcaggtacc tgcttatatt ccggtgcggt gaaaccgtgt gggcatctct tagttttggc
     9241 ttattactta ttgcctggta agagagcaga accaccaacg tttgcctgtt tatcaccttg
     9301 gtaagggtga ggccgcatca agcccagtgc tctacaaggt acttcagatc atagacgctt
     9361 attttctctt agaggtcgcg ttaacctctg ggatctgttt ccactgatga atataaatca
     9421 atcttcctgt taagtctatt gtgtggagaa agagagttga ctttctctct acaggggatc
     9481 acacttgccc ggcgagaagt tgagccccaa aggatgacaa atcccgaggg gctttttgtt
     9541 gattagaaaa gctggttaga ttcctgtgtg gctttccaat ccagctgatc gctgacttcc
     9601 ttctggatca ccctggcaat tcccccctca tcgatggtta tctcccattt gccggaaatc
     9661 tcaccttgtg gggctacatt tacttggact ggttgatatc caccattctg gatagctatg
     9721 ccacccgttg gtgttggtct aatgccagtg tctactgtca atgttggccc tttattaaac
     9781 cagcctctga cctgatcgaa caaaggacca gtatcaccgc caggtgtgaa cagtgcgcca
     9841 gcattgctaa caaatcttcc cagctcaaac aaagcaccct gttttgagtt ctccttactc
     9901 atttcctgcg tcttgccatc aatgaagctt ctttcagcgt tagagcctgc actgatgacc
     9961 gctgctcctg ttgccgttcc acctgaggca gaagccagaa gcttgctcat accagggatt
    10021 ttacctaaca aggctatcag ctgagtgaca agtgaaatca gtccggtaaa tggggttaac
    10081 agagagccga cgactttcag actaatcatt agagcggtca gttttccgat aatctcggca
    10141 acctcggtac cgtttttact aaattgttca gttcttgcag tgataaagcc tgcagcgaaa
    10201 gccatccatt ctacaaagcc tttaatagcc gcgcctatct gcgatcccat ctgtgtggca
    10261 gtgctcccgc cgtcgcccat gacataggtc aggtcttcaa agatagagat caaatgctct
    10321 gacaaagtaa ctacgccatc cgcaggatcc acaacaccca taaactgaat cgccagctca
    10381 ttaaagctgt tcttcatgcg ttgcagtgct acctctggct gcttcataga ttcagcaaaa
    10441 gctttagttt cttttgcagc accccaaatt ttcactaggg aaggaagtag cttgtccagg
    10501 gtgattttgc catctttcat ggccttatcg aattcttggg cattcttgat gattccggca
    10561 tcaatagcgg cctgttggaa gtcctgctga gagcctgcaa aggcttctga cagctggccc
    10621 cgcagctctt ctgccatcac gcgatttttt ccagccattt gagatatagc gacaattgcc
    10681 cggttttgtt gctcgccagt agcacccact gagcgacctt taatagccat agcctcaaac
    10741 aatgcctgtt tatcactctc tttcatggtt ggagtagagg cggcaaagcg catgtagcca
    10801 ccttgcatct cccgctcatc catcccgtag gtgtaggctg tgttcttgat aaaagccatc
    10861 tgctgatcag tcttggcttt atcatccttg ttctgtgcca tcaaaccgaa gtaggcactg
    10921 ttgcgatctg caaggctacc catagcacca ctaacagcac gctgaacacc ccagccagcg
    10981 gcagtaaggc caatggctat cttgcctagt gtggagttga ttttgcccag gttgtcatgc
    11041 accttgctgg cacctgatgc cagtttatcc agtctgctct ggaattttcc ggcctctgct
    11101 gttgccatct tcatttcagc tctgagccga gccattttct gattataggt ctgcaggtta
    11161 atagcccctt tagcataatc agcattaact ctttgaatgc gcccctggaa ctgggcaacc
    11221 agggagccat caaacatact gccaattcgt gcactgttgg cccctacacg tgcttttctg
    11281 tgctgaacat ctccaacact acgatctaac agtttttgtt gtctggcggc ctctctggct
    11341 tgctgctgat agaacttgcg catagaggag agctccggat tattgccccc agtcagacgg
    11401 ctgacagcct gagcacgttg tttcttctgt gcttcctgtt gtttcctctg agctctggct
    11461 atctgctctt ccaccttagc gcgtgttctt tgtgttctga tcacagcctg attagattta
    11521 aaatcagctt tagctatatc gtcggtagct ttcttccagg attttttata atcctcaagc
    11581 cgtttaactt cttttcgcca ggaggattta tcagagtccc agtaaataac gtttgttagt
    11641 ctggcgactt ccttcattgc catctcttac acctcgccct gtcttaaaga ttttgataat
    11701 gaaatagccc ttttcagggc ctctttatca atattatatt tctccgcaag ctcttcgatt
    11761 gagaggcctt ctgtgtgtgg gccactttta ataatggtgg ccccctcttt cgctgcctct
    11821 ggcaggtgac ctattgtcac cccttcgggc gtgtcttcgg agtccttaac cagtttttga
    11881 taaatctttt cgtagatatt catatcattt gcctttgcgt cttagtggac acttatcgcc
    11941 aatagtccac acaaaaatat aatggcgctt gctttcagct ttaggattta ataattcttt
    12001 atgcaaatat ccggctgaaa gcattttatc gatcaggttt ttcactcttt gcccttcctc
    12061 aaatgtttgt gctttaaaat agagatttgg aaagttttgg cctgtatatt ttccatcttc
    12121 atataagcgg gtgcacaccc aacgacataa atcatcgtca ttcagatcac taacgtgggg
    12181 tttatcatcg ctgctaaata acgcctgttt aaaacgggag tatttaaact ccccatcttc
    12241 caggaaatca ccctcttcga attcaactat catttcttca atattcatag gcatatcctc
    12301 tcattgatgc accccccaaa aaatggggag tgcttattta tcaatagctt aatcaagatt
    12361 aaccagtttg agaggtttga cagcttggag catggtctgc atcattccta cacttatttc
    12421 aaagagatct gccatgttct gaaggcttgc cccttctgcc tcaatcattg atttggataa
    12481 ggcagcaatc acaaagccat cctgggcgct gttccacatg ctgataagag gatcatcacc
    12541 gtgttggcgc ataaggtcct gaatcatcca caatctacat gccataacca ccaaatcgac
    12601 atagaaaaca tcaccattag cggacttgca ttcagcaaaa gcgtggtgtc tgtgttttaa
    12661 gaagtgctct ttgtcacggg taaatgccag ggtgttggta aacttgccat ctaaaagatc
    12721 ctggcgaact tttgaatctt cagcgtcgat agctttgata acctgggata ataatttagc
    12781 gatttgagac atttggaatt ccttaaatta gagccccgga aggggttaaa gtgagtcgaa
    12841 aataaataaa tcgaagagat aaaacttagg ctttctgggc cattgctttc tggactttat
    12901 ttctatggcg gcggtagatg aatataaatt cgcgataagc acgactaccg ggcttaggca
    12961 aacgttgatt aagctcctta cgtggcagga tttcactcaa caagccccag atatgaaagc
    13021 aggttacttt taattctttt gctatctctt caatattcat cccgcaattc cacattaaaa
    13081 gagatttgcg gcggcgatct tcgaagacat ttctatctct atctatttcg ttatagacac
    13141 tgaggggaac tcgacaccgt ggattccagg actcattata tagtttttgg actttctcaa
    13201 gtttaacgcc tgtatattcc gccacgaatt caggggtaaa gccctcgact aataagtgtt
    13261 tagcctgtcg tgttggttga tcatcactgg ggagatattt ctgcagtgct accagttttt
    13321 caaattggga atcagaaaga tcagatttga acatgattaa ttctccgaac gtttaaaaga
    13381 tcagggcaat caatctagtt ctagtaattc tatgtttagt ttatttagtt tatttggggg
    13441 tattaataag ggatgtcaga atcgttttct tcatctgctg gatcttcagc taaccaacca
    13501 caatattctt cccaatatga aaaatgttgt gatagttcat cttcaaaata tttttccttt
    13561 tccaggtaat aatcaaaatc gccatacatt ttaaacttcc ttaaac
//
LOCUS       DAZFJF010000114        13565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFJF010000114 DAZFJF010000000
VERSION     DAZFJF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.114"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            196..1827
                     /gene="oppA"
                     /locus_tag="ACHKAU_003815"
     CDS             196..1827
                     /gene="oppA"
                     /locus_tag="ACHKAU_003815"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO5532796.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1913..2833
                     /gene="oppB"
                     /locus_tag="ACHKAU_003816"
     CDS             1913..2833
                     /gene="oppB"
                     /locus_tag="ACHKAU_003816"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO5532797.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2848..3756
                     /gene="oppC"
                     /locus_tag="ACHKAU_003817"
     CDS             2848..3756
                     /gene="oppC"
                     /locus_tag="ACHKAU_003817"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO5532798.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3768..4781
                     /gene="oppD"
                     /locus_tag="ACHKAU_003818"
     CDS             3768..4781
                     /gene="oppD"
                     /locus_tag="ACHKAU_003818"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO5532799.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4778..5782
                     /gene="oppF"
                     /locus_tag="ACHKAU_003819"
     CDS             4778..5782
                     /gene="oppF"
                     /locus_tag="ACHKAU_003819"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO5532800.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5835..6164)
                     /gene="yciU"
                     /locus_tag="ACHKAU_003820"
     CDS             complement(5835..6164)
                     /gene="yciU"
                     /locus_tag="ACHKAU_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO5532801.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6199..7659)
                     /gene="cls"
                     /locus_tag="ACHKAU_003821"
     CDS             complement(6199..7659)
                     /gene="cls"
                     /locus_tag="ACHKAU_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO5532802.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7802..7975
                     /gene="yciY"
                     /locus_tag="ACHKAU_003822"
     CDS             7802..7975
                     /gene="yciY"
                     /locus_tag="ACHKAU_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO5532803.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8030..9283)
                     /gene="kch"
                     /locus_tag="ACHKAU_003823"
     CDS             complement(8030..9283)
                     /gene="kch"
                     /locus_tag="ACHKAU_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO5532804.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9582..9878)
                     /gene="yciI"
                     /locus_tag="ACHKAU_003824"
     CDS             complement(9582..9878)
                     /gene="yciI"
                     /locus_tag="ACHKAU_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO5532805.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10102..10821
                     /gene="tonB"
                     /locus_tag="ACHKAU_003825"
     CDS             10102..10821
                     /gene="tonB"
                     /locus_tag="ACHKAU_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO5532806.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(10861..11259)
                     /gene="yciA"
                     /locus_tag="ACHKAU_003826"
     CDS             complement(10861..11259)
                     /gene="yciA"
                     /locus_tag="ACHKAU_003826"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO5532807.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11364..11903)
                     /gene="yciB"
                     /locus_tag="ACHKAU_003827"
     CDS             complement(11364..11903)
                     /gene="yciB"
                     /locus_tag="ACHKAU_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO5532808.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(11933..12676)
                     /gene="yciC"
                     /locus_tag="ACHKAU_003828"
     CDS             complement(11933..12676)
                     /gene="yciC"
                     /locus_tag="ACHKAU_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO5532809.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12704..12793)
                     /locus_tag="ACHKAU_003829"
                     /pseudo
     CDS             complement(12704..12793)
                     /locus_tag="ACHKAU_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13033..>13565
                     /gene="ompW"
                     /locus_tag="ACHKAU_003830"
     CDS             13033..>13565
                     /gene="ompW"
                     /locus_tag="ACHKAU_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO5532810.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWY"
BASE COUNT         3616 a         3216 c         3345 g         3388 t
ORIGIN      
        1 ctcgacaggg gagacacagt acgaatcgac ataaggtgat cgtctgaatc accagaataa
       61 ataaagtcgg tgatagtaat acgtaacgat aaagtaacct gacagcagaa agtctccgag
      121 cctgtgcagg gtcccaatcc gggattacac atgctggtta ataccagtaa ttataatgag
      181 ggagtccaaa aaacaatgac caacatcacc aagagaagtt tagtagcagc tggcgttctg
      241 gctgcgctaa tggcagggaa tgtcgcgctg gcagctgatg tacccgcagg cgtcacactg
      301 gcggaaaaac aaacactggt acgtaacaat ggttcagaag ttcagtcatt agatccgcac
      361 aaaattgaag gtgttccgga gtctaatatc agccgagacc tgtttgaagg cttactggtc
      421 agcgatcttg acggtcatcc agcacctggc gtcgctgaat cctgggataa taaagacgcg
      481 aaagtctgga ccttccattt gcgtaaagat gcgaaatggt ctgatggcac gccagtcaca
      541 gcacaagact ttgtgtatag ctggcaacgt tctgttgatc cgaacactgc ttctccgtat
      601 gccagttatc tgcaatatgg gcatatcgcc ggtattgatg aaattcttga agggaaaaaa
      661 ccgattaccg atctcggcgt gaaagctatt gatgatcaca cattagaagt caccttaagt
      721 gaacccgttc cgtacttcta taaattactt gttcacccat caacttcacc ggtgccaaaa
      781 gccgctatcg agaaattcgg cgaaaaatgg acccagcctg gtaatatcgt caccaacggt
      841 gcctatacct taaaagattg ggtcgtaaac gaacgaatcg ttcttgaacg cagcccgacc
      901 tactggaaca acgcgaaaac cgttattaac caggtaacct atttgcctat tgcttctgaa
      961 gttaccgatg tcaaccgcta ccgtagtggt gaaatcgaca tgacttataa caacatgccg
     1021 atcgaattgt tccagaagct gaaaaaagag atcccggacg aagttcacgt tgatccatac
     1081 ctgtgcactt actattacga aattaacaac cagaaaccgc cattcaacga tgtgcgtgtg
     1141 cgtaccgcac tgaaactagg tatggaccgc gatatcattg ttaataaagt gaaagcgcag
     1201 ggcgacatgc ccgcctatgg ttacactcca ccgtatactg atggcgcaaa attgactcag
     1261 ccggaatggt ttggctggag ccaggaaaaa cgtaacgaag aagcgaaaaa actgctggct
     1321 gaagcgggtt ataccgcaga caaaccgttg accatcaacc tgttgtataa cacctccgat
     1381 ctgcataaaa agctggcgat tgctgcctct tcattgtgga agaaaaacat tggtgtaaac
     1441 gtcaaactgg ttaaccagga gtggaaaacg ttcctcgaca cccgtcacca gggtactttt
     1501 gatgtggccc gtgcaggctg gtgtgctgac tacaacgaac caacttcctt cctgaacacc
     1561 atgctttcga acagctcgat gaataccgcg cattataaga gcccggcctt tgacagcatt
     1621 atggcggaaa cgctgaaagt gactgacgag gcgcagcgca cagctctgta cactaaagca
     1681 gaacaacagc tggataagga ttcggccatt gttcctgttt attactacgt gaatgcgcgt
     1741 ctggtgaaac cgtgggttgg tggctatacc ggcaaagatc cgctggataa tacctatacc
     1801 cggaatatgt acattgtgaa gcactaatgg caatacgtgg ggcaggagtg tcctgctcca
     1861 cggtgtctga tttttatcgc attacagaag gcacaggcca gaaggtaggg caatgttaaa
     1921 atttattcta cgtcgctgtc tggaagcgat tccgacgcta tttattctta ttactatttc
     1981 gttctttatg atgcgcctcg cgccgggaag cccttttacc ggcgaacgta ctttaccgcc
     2041 agaagtgatg gccaatatcg aagagaaata tcatcttaat gatccgatca tgacacagta
     2101 tttcagctac ctgaaacaac tggcgcacgg tgatttcggt ccatcgttta aatataaaga
     2161 ttattcggtc aatgacttgg ttgcatccag ttttcccgtt tctgccaaac tgggagccgc
     2221 agcatttttc cttgcggtaa tactgggggt tagtgctggc gttattgccg cattaaaaca
     2281 aaacaccaaa tgggactata ccgtgatggg gctggcaatg accggggttg ttatccccag
     2341 ttttgtggtt gcaccattat tagtcatgat atttgcgatc attttgcatt ggctgccggg
     2401 cggtggctgg aatggtgggg cgcttaaatt catgatattg ccgatggtgg cgttgtcact
     2461 cgcttatatc gccagtattg cgcgtattac ccgtggctct atgattgaag tattacactc
     2521 caattttatt cgtactgccc gggcgaaagg gttacctatg cggcggatca ttttacgtca
     2581 cgcattaaaa cctgctctgt tacccgtgct ctcttatatg ggccccgcat ttgtcggcat
     2641 tattaccggt tctatggtta tcgaaaccat ttatggtttg ccggggattg gacaactgtt
     2701 cgttaatggc gcattgaacc gtgactattc cttagtgtta agcctgacca tcctggttgg
     2761 tgctttaacc attttgttta atgccattgt cgatgtgcta tatgcggtta tcgacccgaa
     2821 aatccgttac tgatactgga gctcgcgatg atgttaagta agaaaaacag cgagacgctg
     2881 gaaaatttca gtgaaaagct ggaggtcgaa gggcgcagct tgtggcagga cgcacgtcga
     2941 cgttttatgc ataaccgtgc ggcggttgcc agtctgatag tgctggtgct gatcgcgtta
     3001 tttgtaatcc tggcaccgat gctttcccag tttgcctatg acgatactga ctgggcgatg
     3061 atgtccagcg ccccggatat ggagtccggt cactactttg gtactgactc atccggtcgc
     3121 gacctgcttg tgcgcgttgc gattggcggg cgtatctcac tcatggtcgg tgttgctgcg
     3181 gcactggtgg ctgtggtcgt cgggacactt tatggttcgc tttccggtta tctgggcggt
     3241 aaagtggatt cggtaatgat gcgtctgctg gaaatcctca actccttccc attcatgttc
     3301 ttcgtcattt tgctggtgac ctttttcggt caaaacatcc tgctgatttt cgtggcgata
     3361 ggcatggttt cctggctgga tatggctcgt attgtgcgtg ggcaaaccct gagtctgaag
     3421 cgcaaagagt ttattgaggc ggcacaagtt ggcggtgtat cgacatcggg cattgttatt
     3481 cgccacattg tgccgaatgt actcggtgtg gtggtggtct acgcatcact tctggtgccc
     3541 agcatgatcc tctttgaatc tttccttagc ttcctggggt tgggtacgca agagccgtta
     3601 agcagctggg gtgcattgct gagtgatggc gcgaactcga tggaagtctc tccatggtta
     3661 ttgttgttcc cagcgggatt cctcgtggtg acgctgtttt gtttcaactt tatcggcgat
     3721 ggcttgcgtg atgccctcga cccgaaagat cgttaaggag tgcagccatg agcgtaattg
     3781 aaactgcaac tgtgccgctc gcacaacaac aggctgacgc actgctgaac gtgaaagatt
     3841 tgcgtgtcac ctttagtacc ccggacggcg acgtcacggc ggtcaatgat ttgaattttt
     3901 ccctacgtgc cggagaaacg ctgggtattg taggtgagtc tggttcgggt aaatcgcaaa
     3961 ctgcatttgc gttgatgggc ctgttggctg ccaacggacg tattggcgga tcggcaacct
     4021 tcaatgggcg tgaaatcctc aatttgccag agcatgaact caataaactg cgcgctgaac
     4081 aaatctcaat gatttttcag gacccaatga cttcgttgaa tccctatatg cgcgtcggtg
     4141 agcagttaat ggaagtgctg atgctgcata agaacatgag caaagctgag gcgtttgaag
     4201 agtcggtgcg gatgctcgat gcggtaaaaa tgccggaagc gcgtaaacgc atgaaaatgt
     4261 acccgcacga attttctggc ggcatgcgtc agcgagtcat gattgcgatg gcattgctat
     4321 gtcgacctaa gctactgatt gcggatgaac ccactactgc gctggacgtc accgtacagg
     4381 cgcagatcat gacgctatta aatgaactga agcgggaatt taataccgcc atcattatga
     4441 tcacccacga tcttggcgtg gtggcgggga tctgtgacaa agtgctggta atgtacgccg
     4501 ggcgcacgat ggaatatggc aatgcgcgcg atgtctttta tcaacccgtt catccttatt
     4561 ctatcggttt gctcaacgcg gtgccgcgtc ttgatgcgga aggtgaaaca atgttgacca
     4621 tcccgggtaa tccgccaaac ttgctgcgat taccgaaagg ttgcccgttc cagccacgtt
     4681 gtccacatgc gatggaaatt tgtagtagcg caccgccgct ggaagagttt acgcctggcc
     4741 gtctgcgtgc ttgctttaaa ccggtggagg aactgttatg aatgctgtaa ctgaaggaag
     4801 aaaagtcctc cttgaaatcg ccgatcttaa agtgcacttt gaaatcaaag atggcaaaca
     4861 gtggttctgg caaccgccga aaacgctcaa agccgtcgat ggtgtcactc ttcgcctgta
     4921 tgaaggggaa acattaggtg tggtagggga atcgggatgc ggtaagtcca cctttgctcg
     4981 cgccatcatc ggtttggtca aggcgaccga cggtcatgtt gcctggttag gtaaagagtt
     5041 gctgggcatg aagcccgatg aatggcgtgc cgttcgcagt gatattcaga tgattttcca
     5101 ggatccgttg gcatcgctaa acccgcgtat gaccatcggc gagatcatcg ctgaaccact
     5161 gcgtacttat catccgaaaa tgtcacgcca ggaagttcgc gagcgcgtga aggcgatgat
     5221 gctgaaagtc gggttattgc ctaacctgat taaccgctat ccgcatgagt tctccggtgg
     5281 gcagtgccag cgtatcggga ttgctcgtgc tcttattctt gaaccgaagc tgattatctg
     5341 cgatgagccg gtttcggcgc tggacgtgtc aattcaggcg caggtggtca acctgctcca
     5401 gcagctgcaa cgtgagatgg gattgtcatt aatttttatc gctcatgacc tggccgtggt
     5461 aaaacacatt tccgatcgtg tgttggtgat gtatctcggc catgcggtag aactggggac
     5521 ctatgatgag gtctaccaca atccactaca tccttacacc agggcattga tgtcggcagt
     5581 ccccatacct gatccggatc tggagaagaa caaaaccatc cagttactgg aaggggaatt
     5641 accgtcgccg atcaacccgc cttccggttg tgttttccgt acccgttgcc cgattgccgg
     5701 tccggagtgc gccaaaacac gtcctgttct ggaggggagt ttcagacacg ccgtttcttg
     5761 cctgaaagtc gatccgcttt aaacacaata agggctgaca gttgtcagcc ctttttcacg
     5821 ctaaaagcga ttatttattc ccgccagatg atatggcaaa gtttgtgatc tttttcgcgg
     5881 cataacagaa tgcgggcgaa aacatcgttg atttcaccat cttcactgtc cgccagacca
     5941 atcaccactt cggcaaaaaa gtctgggttc aaatcgaaat caacatgttc ctgccagtct
     6001 tccgccggat cgaataactc cgcgccgccg cgctcttcaa actgaagatt gaacagcaga
     6061 acatcagctg gatcgagatt gtcggcagcc agttcgagaa aaatatcgta ggcctgctca
     6121 agcgtttcat cttcagtcag gcgattgttt agatccatat ccatagtcac tacctgttta
     6181 acctctgttg gcgacgtttt acagcaacgg actgaagaag taaaacagtc gctcggcgac
     6241 acgttgccat aatggacgtt ttagccataa acgggcatcg agcagacgtg aacgcgaaat
     6301 ataatcgtcc tgaacggcgg caaggtctgc accaaaacct ttatcgtcga ttgccagggt
     6361 aatctcgaaa ttcagccaca gactccgcat atcaaggtta acggtgccaa ccagacttag
     6421 ttcgccatcg accagcacgc tcttggtatg cagtaacccg ccttcaaact gataaatttt
     6481 aaccccagca gcaagcagtt ccgtaaagaa tgcgcgactg gcccagccga ccagcatcga
     6541 gtcatttttt cgcggaagga taatactgac atccaccccg cgctgcgccg ccgtgcaaat
     6601 cgcatgaagt aaatcatcgc ttggcacaaa gtagggcgtg gtcatgatca aatattcacg
     6661 cgccgaataa gccgcagtca ataatgcctg gtgaatgaga tcttccggaa agccggggcc
     6721 agaagcaatt gtgtgaatgg tgtgaccgct ggcctgttca aacggcataa tattgacatc
     6781 tggtggtggc ggcagaatac gttttccggt ttcaatctcc cagtcgcagg aataaataat
     6841 ccccatcgcg gtggcgatgg ggccttccat acgcgccatc agatcaatcc attgccctac
     6901 gcccgcatct tgtttgaagt agcgaggatc gaccatattc atgctgccgg tgtacgcgat
     6961 gtaattatcg atcatgatca tcttgcgatg ttggcgcagg tccatacggc gtaaaaacac
     7021 acgcatcaga ttgaccttta aggcttcgac cacttcaata ccggcattac gcattagctc
     7081 gggccacggg ctgcggaaaa aagccacact cccggcggag tcgagcatca atcggcaatg
     7141 aataccacgc cgtgcagccg ccattaatga ttcagccacc tggtccgcca tgccgccggg
     7201 ctgccagata taaaacacca tctcaatatt atggcgcgcg agctggatgt cgcggattaa
     7261 cgcctgcatc acatcatctg actcggtcat cagttgtagc tgattccctt tgaccccagc
     7321 gatcccctga cgacgctcgc aaagcttgaa taatggcgca gcgacactgc tattttcttc
     7381 ggcgaagata tgcttacagg ctttaaggtc gttaagccat tttgcggtgg aaggccacat
     7441 cgctctggcg cgctcagcgc ggcgtttgcc taaatggagc tcgccaacgg caagataggc
     7501 aataattccg actaacggca gaatgtaaat aatcaacagc caggccatcg cggagggaac
     7561 tgcgcgtcgt ttcattagaa tgcgtaaagt tacgcctgca atgagcaacc agtatcccag
     7621 aatggccaac caactcacca acgtataaac ggttgtcata gattagaaat ccttttgaaa
     7681 gcgcattgtt atgagtttac gcatatggat tcatctggca aataaaaacg cgggaaaagc
     7741 actggtcagc gtcgggggtg ggggtataat gaccattctg ttattgcata gagtagttaa
     7801 catgaagcgg agtagaacgg aagtggggcg ctggcgcatg cagcgtcagg ctagccgacg
     7861 taaatcgcgt tggcttgagg ggcaatcgcg ccgaaatatg cgtatccaca gcatcaggaa
     7921 gtgcattcta aacaaacagc gtaactcgtt attgtttgcg atctacaata tctaaatata
     7981 ctgggcacca ttgcggtgcc caataagttt actctttcgt ctttaaactc tatttttgca
     8041 ctgattcttt actgtcagct ttcgtttctt gttcatcgtt atcgctgaaa atgccatgac
     8101 cggagttcaa caacattgaa acgagcatat cattattaat ctcttcacca tttaaaactc
     8161 gcgccagaat ttcgctgcca aacagttgcg gtgagagaat gatatccgga tgtaccatct
     8221 taatcttatt caggtttttg ctatcactga cggcgagaac tgttttgaca tcactgctca
     8281 tatcttttgc cgagagtaca acaaacgcgt tatctgcatc gttatcactc agcgccagaa
     8341 tggcccggca acgatcgatt cccgctttct ttaatactga actgtcatta ctgtcaccgg
     8401 ggataacatc agcgttatcg cctaagcgtt gctcaagttg cttgatatca tcttcaggca
     8461 agttgctgat aaccgttacg ttttgtccgc gttgattcag ttgcaggatc gtattgatgg
     8521 cgagaatcga atgtccgcaa acaataaaat gatctttacg atgcattgta tgattgtttc
     8581 cttttacaag tttgttgaat cccccgcgga taagcggacc aaaaattgag gtcatagatg
     8641 tggcaaaaac ggtaatgccg gaaataatga ccgaaatagt gaacaatcgt gctgattcag
     8701 aaacagggac aatatcgcca tagccgacgg ttgacatggt ttctatcgaa aaatagaacg
     8761 cggtcatcaa actttctatt cgcggattaa aaccttcgct taaataaagc gcaccgtagg
     8821 ttgagtaaaa cagtaatgtc gtgaaactaa taaatgcaaa aattgtcccg gctgcggcgc
     8881 tactgtggga gaagtctttg cgcagtatca gcaaaaacac cagcgtaaaa atgcaaaatc
     8941 caatactaaa tttcagccag ggataaaaat gcagggtgta aattagcgct atcaacaaca
     9001 aaatgatact gattgcccat gcgagctttg cccggaacag taagccaatg gagttcagga
     9061 caagaaagac gccgagcata aatagtgggg cgtttgccag caaggagaga tccaactctg
     9121 aaaatgcttt gatatggaaa atatcaagca aattgaccga cataccatag attgttttaa
     9181 aaataagcaa tccatttaaa aagacggcca gcgcgagaat gtcgtgccga agggtcaccc
     9241 ataaatttgt tgctgtttgt ttgaatgtag cccagtgact cactgatacc tctgtaacca
     9301 caattttgtt taattatctt agcgtgcaca ttttctgaac atacatgcag cgcgtgaatg
     9361 tgttaaacaa tagtagatga ctttatcggc agtaatcaaa ggttttgagc gagcaactct
     9421 gcgtcaggaa agggcatttt cctgaatatg ttcacatcat ataaagaaat acagataaag
     9481 ttattatctg cttgtggtgg tgaatgcact gaccggctat aaggaaaggc caaacaagaa
     9541 cacggttgca aaaaccgtgc ccttaaatat tgaatctcta ttcagaacac tttcttaaat
     9601 ggtttcactg aaacgtgttc atagactcct gccgctacgt acgggtcagc atcggcccag
     9661 gcctgagctg cttccagcga ttcaaattca gcaataacgg ttgagccagt aaatcccgca
     9721 gcccctggat cgttactgtc taccgctggc attggaccag ctgtcaacaa acgaccttca
     9781 tcatgcagta actgtaaacg tgctaaatgt gccggacgaa cggaaaggcg tttttcgagg
     9841 ctatcagctt tgtcttgagc gtaaataaca tacaacacgg gcacaactcc ttgttcggga
     9901 aagttgtaaa gtacgttatt tgaaagggcg aagatctgca acggaaagat gatgtctttg
     9961 ttaagaccat gcataaatta aggtcaatta cgccaaaaat gacattttca ctgatcctga
    10021 tcgtcttgcc ttattgaata tgattgctat ttgcatttaa aatcgagacc tggtttttct
    10081 actgaaatga ttatgacttc aatgaccctt gatttacctc gccgcttccc ctggccgacg
    10141 ttactttcgg tctgcattca tggtgctgtt gtggcgggtc tgctctatac ctcggtacat
    10201 caggttattg aactacctgc gcctgcgcag ccaatttctg tcacgatggt tgcgcctgct
    10261 gatctcgaac cgccacaagc cgttcagccg ccaccggagc cggtggtaga gccagaaccg
    10321 gaacctgagc cgatccccga accgccaaaa gaagcaccgg tggtcattga aaagccgaag
    10381 ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac aggagcagcc aaaacgcgat
    10441 atcaaacccg tagagtcgcg tccggcatca ccgtttgaaa atacggcacc cgcgcgcccc
    10501 acatcaagta cggcaacggc tgcaaccagc aagccggtta ccagtgtggc ttcaggacca
    10561 cgcgcattaa gccgtaatca gccgcagtat ccggcacgag cacaggcatt gcgcattgaa
    10621 gggcaggtta aagttaaatt tgacgtcacg ccggatggtc gcgtggataa cgtacaaatc
    10681 ctctcagcca agcctgcgaa catgtttgag cgtgaggtga aaaatgcgat gcgcagatgg
    10741 cgttatgagc cgggtaagcc aggcagtggg attgtggtga atatcctgtt taaaattaac
    10801 ggcaccaccg aaattcagta agcagaaagt caaaagcctc cgaccggagg cttttgacta
    10861 ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca accgcgacat acttaaataa
    10921 tgcttctgtc gctttatagc gttgcccgat tggttcagac gctacttttt tcacccacac
    10981 ttcaatatta atgctgaccg atgtcgtccc tttctggaca cagcgtgcat agcagcacac
    11041 cacatcgccg accgcaaccg gccgtaagaa agtcattcct tcaacccgca cagtcactac
    11101 gcgaccgtgg gcaatttctt tcgccagaat agcgccgcca atatccattt gtgacattaa
    11161 ccaaccacca aagatgtcac cattggcatt ggtatcggcg ggcatggcta aagtacgtaa
    11221 aacaagatcg ccctgaggga cgttatgtgt tgtagacatg gtaaaaccga cttaaaagga
    11281 aaatcacagg cgcgatgcta ctatgatttt cctgaggaga acagaggaga tacagcgcca
    11341 gccccgaagg actggcagtc tggttaggat ttatcttcct gcggcatgtg gcggtagata
    11401 tagataccgc ttaacaatgt gaagattaag gtaagggcgg tcaggccaaa gactttaaag
    11461 ttgacccaaa tattttgcgg cagccagaat gcgatgtaga tgtttgccag accgcaaagg
    11521 ataaagaaaa cagcccaggc cagatttagc ttcgaccata ccggttgcgg cagcgtgagt
    11581 tctttaccca gcatccgctg aattagcggc tttttcatca cccattggct gactaacagg
    11641 gctcccgcaa acagagcata aatgactgta accttccatt taataaactc atcattgtgg
    11701 aagaacagcg tcaagccacc gaagacgacc accagaacaa aagtgatcag ggccatcttc
    11761 tcaaccttac gaaagcgaac ccagctatag ataagcacaa tcgccgtggc gacgatcagc
    11821 gccgcagtag ccgcatagat gtcataaatc ttgtaaaacg cgaaaaagac aaccagcggt
    11881 aaaaaatcaa gaaactgctt cattttacga ttccgtaatc aaatgcttaa ggttattggc
    11941 gaatcaacat atacaggcgg aacagataga tgagcaatat ggcagaaatc aggttgctca
    12001 aggtgttcgc cagtacggca ccaatttccg gggttaatgc ggcaaaagaa gaggcaaaaa
    12061 gcagcaacag tgtttttgcc agcaaccagc tcagtactgc gggtgccacc agacgcatat
    12121 tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa aatgcccatt ttgtcctgta
    12181 ccagcatcac cggtgccagg gccagtaaaa tggccatgat aattcccgga acgaccacca
    12241 gcatgatgcc aatctgtact aaaagggtag tcagaaaaat cagaataaat aacttgggca
    12301 atatcggcgc actggcacca atagctcgca gcgcactgac tctctgaccc gcagacacca
    12361 gctggataat taatattacg cctccggcga gaatggcgtt accgattaat cctgaaaaag
    12421 tggacgccgc tgaagcctgc agcaaaattt gttgctgttc cggtgacata ttctgaacca
    12481 ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc attgagctgc gcaagctgtg
    12541 catcactggg tgagaaaaca tgccctaaca ccactgtgat aaacgcacat agcaacgata
    12601 ccaacagaat ggtcataaat tgattacgga agaaatttcc ggtgtcacgg tatacggact
    12661 gcgccgtgat agacatgcac tctccttgag ttttatgcag gtgttaatta gcgggcaatt
    12721 gtaccctggt taagcctcgg gtggcagcat caggtcttgt atggaaaagc atatctttgt
    12781 aaagcggagg taattgctgg ccttcaggcg tgggcaaacc gcggcaagtg gcgatcgagt
    12841 taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa tcacattaac caggattctc
    12901 aatgcaactt ctaaattaat ccagatcaat aaatggtgaa ttatcatatg taatgtgatc
    12961 tatgtaggat catttgttac tccaatgtag gtatattcgt cacgttttta taaccataac
    13021 gacggagcgg atatgaaaaa gttaacagtg gcggctttgg cagtaacaac tcttctttct
    13081 ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc gtgcaggttc tgcaaccgta
    13141 cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg gtggattcag cgtgaccaat
    13201 aacactcaac tgggcttgac gtttacttat atggcgaccg acaacattgg tgtggaatta
    13261 ctggcagcga cgccgttccg ccataaaatc ggcacccggg cgaccggcga tattgcaacc
    13321 gttcaccatc tgccgccaac actgatggcg cagtggtatt ttggtgatgc cagcagcaaa
    13381 ttccgtcctt acgttggggc cggtattaac tacaccacct tctttgataa tggatttaac
    13441 gatcatggca aagaggcggg gctttccgat ctcagtctga aagattcctg gggagctgcc
    13501 gggcaggtgg gggttgatta tctgattaac cgtgactggt tggttaacat gtcagtgtgg
    13561 tacat
//
LOCUS       DAZFJF010000115        13391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFJF010000115 DAZFJF010000000
VERSION     DAZFJF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.115"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..1287)
                     /locus_tag="ACHKAU_003831"
     CDS             complement(25..1287)
                     /locus_tag="ACHKAU_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5532811.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFAKFMGK"
     gene            complement(1451..1535)
                     /locus_tag="ACHKAU_003832"
     tRNA            complement(1451..1535)
                     /locus_tag="ACHKAU_003832"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1499..1501),aa:Leu,seq:caa)
     gene            1731..2750
                     /gene="ahr"
                     /locus_tag="ACHKAU_003833"
     CDS             1731..2750
                     /gene="ahr"
                     /locus_tag="ACHKAU_003833"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO5532812.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2880..4382
                     /gene="yjgR"
                     /locus_tag="ACHKAU_003834"
     CDS             2880..4382
                     /gene="yjgR"
                     /locus_tag="ACHKAU_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO5532813.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4501..5583)
                     /gene="lptG"
                     /locus_tag="ACHKAU_003835"
     CDS             complement(4501..5583)
                     /gene="lptG"
                     /locus_tag="ACHKAU_003835"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5532814.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5583..6683)
                     /gene="lptF"
                     /locus_tag="ACHKAU_003836"
     CDS             complement(5583..6683)
                     /gene="lptF"
                     /locus_tag="ACHKAU_003836"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5532815.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6746..6796)
                     /gene="ytgA"
                     /locus_tag="ACHKAU_003837"
     CDS             complement(6746..6796)
                     /gene="ytgA"
                     /locus_tag="ACHKAU_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO5532816.1"
                     /translation="MSIIILATISTTQENK"
     gene            6950..8461
                     /gene="pepA"
                     /locus_tag="ACHKAU_003838"
     CDS             6950..8461
                     /gene="pepA"
                     /locus_tag="ACHKAU_003838"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO5532817.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8816..9259
                     /gene="holC"
                     /locus_tag="ACHKAU_003839"
     CDS             8816..9259
                     /gene="holC"
                     /locus_tag="ACHKAU_003839"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO5532818.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9259..12114
                     /gene="valS"
                     /locus_tag="ACHKAU_003840"
     CDS             9259..12114
                     /gene="valS"
                     /locus_tag="ACHKAU_003840"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO5532819.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12168..13364)
                     /gene="yjgN"
                     /locus_tag="ACHKAU_003841"
                     /pseudo
     CDS             complement(12168..13364)
                     /gene="yjgN"
                     /locus_tag="ACHKAU_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
BASE COUNT         3422 a         3442 c         3505 g         3022 t
ORIGIN      
        1 ttaatgtata atatttcagt tacattattt ccccatgaat ttagcaaatt cataaggtgt
       61 aacagagtta tttcgattgg cctcaagaaa atcagcccac cattgaacca ttaatctacg
      121 ttcgtctaaa tgctcagagg tatgaatata tgctgcacgc acattgttac gctctgtatg
      181 actcaactgc cgttctatcg cgtcatcgct ccataagcct gattcaccaa gcgcgccacg
      241 cgccatcgtc ctaaatccat gcccacagac ctcagttttc gtgtcatacc ccatcgcacg
      301 caaggcgcta tttactgtgt tctcactcat aactttggcc gcgtcgtgat cccctggaaa
      361 aagtaactct ttatcgccac taatctgctt gagttgctct aacaaaacca tcgcctgtcg
      421 actaagagga acgatatgct cctctttcat cttcatgcca cgatacgagt aacgcacgcc
      481 tttaatttct tctcgctttg caggtatacg ccaaagagat ttatcgaagt cgaactcatc
      541 ccatcgcgcg aaacgtaact cactggaacg tacaaaagtt agtaaggaaa gctcgaccgc
      601 gatccttgtc attacacgac cacgatatgc agcaagacgt gcaagaaact cagagaagcg
      661 gctagagggt aaagctggat agtgtcgtgc tttggttgta gataacgcac cggccatgtc
      721 actggctgga ttagagtcga tgtaatcgtt ctgtacggca taacgcataa tggccgtgac
      781 gcgctgttgc aggcgctgag cgacatcgtg tttgccactg gcatcaactt ttttaatcgg
      841 ggctaacagg tggctggttt taagctgccg aatgtcggac gaaccgatat gaggaaaaat
      901 ataaagctca agatagcgca gaatgcgtga tcgatggtct tcactccagc gcttattact
      961 ggcatgccat tcacgagcaa ttgtttcgaa agtgtatgcg cccgaattct cagcctgagc
     1021 ttctttctgc tcggcttttg ggtcaatgcc ctgcactaag agctttttag cctcgtcacg
     1081 ctttgctctt gcctgagcaa gtgtcaccat gggccaaaca ccaaaagcga ggcgatcctc
     1141 ttttttgtca gaggggcgtc tgtatttcat gcgccagtat ttagaaccct tagccgaaac
     1201 ctcgagatac aaaccgccgc catcggccat tttgtaggtt ttctcttttg gctttgctgt
     1261 ctcgacctgt ctggcgttga gcttcatttg ggggcacatt tctaatcgaa gttaagatgc
     1321 ccccaattat gcccccaata gcatccggat ttcaacggac tagctcggac agctcaggac
     1381 agaaaaaccg ctacaggcat tgattttaaa gggtttgctg gattttctcg gatggtcttg
     1441 gaagtactaa tggtgccgaa ggccggactc gaaccggcac gtatttctac ggttgatttt
     1501 gaatcaactg cgtctaccga tttcgccact tcggcactga agaggtatgc ggaaaacgtt
     1561 gtggattata cctgtcgcac gccaccatgc aagcgccagc ttgcacccac ctcgctaagt
     1621 gctgaaattt tcagcattac actcagcgat atgtaacctt tgtcacactc caggcacccc
     1681 gccctgccat gctctacact tcccaaacaa caccagagaa ggacccaaaa atgtcgatga
     1741 taaaaagcta tgccgcaaaa gaagcgggcg gcgaactgga agtttatgag tacgatcccg
     1801 gtgagctgaa gccacaagat gttgaagtgc aggtggatta ctgcgggatt tgtcattccg
     1861 atctgtcgat gatcgataac gaatggggat tttcacaata tccgctggtt gccgggcatg
     1921 aggtgattgg gcgcgtggtg gcactcggga gcgccgcgca ggataaaggt ttgcaggtcg
     1981 gtcagcgtgt cgggattggc tggacggcgc gtagctgtgg tcactgcgac acctgtatta
     2041 gcggtaatca gatcaactgc gagcaaggtg cggtgccgac gattatgaat cgcggtggct
     2101 ttgccgagaa gttgcgtgcg gactggcaat gggtgattcc actgccagaa aatattgata
     2161 tcgagtccgc cgggccgctg ttgtgcggcg gtatcacggt ctttaaacca ctgttgatgc
     2221 accatatcac tgctaccagc cgcgttgggg taattggtat tggcgggctg gggcatatcg
     2281 ctataaaact tctgcacgca atgggatgcg aggtgacggc ctttagttct aatccggcga
     2341 aagagcagga agtactggcg atgggtaccg ataaagtggt gaatagccgc gatccgcagg
     2401 cactgaaagc cctgtcgggg cagtttgatc tcattatcaa tactgtgaac gtcagcctcg
     2461 actggcagcc ttattttgag gcgctgacct atggcggtaa tttccatacg gtcggtgcgg
     2521 ttctcacgcc gctgtctgtt ccggccttta cgttaattgc gggcgaccgc agcatctctg
     2581 gttctgctac cggcacgcct tatgagctgc gtaagctgat gcgttttgcc gcccgcagca
     2641 aggttgcgcc gaccaccgaa ctgttcccga tgtcgaaaat taacgacgcc atccagcatg
     2701 tacgcgatgg taaagcgcgc taccgcgtgg tcctgaaagc cgacttctga tccctttgcc
     2761 gggagcctgt cgcccggcac ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt
     2821 agcggtgaag agtgcacagc cgttgcctat actccaggca ggaaactgga ggaaatctca
     2881 tgagcgaacc cctgttaatt gcccgcacgc cggacacaga actgttttta ctgccgggaa
     2941 tggctaaccg tcacgggctg attactggcg caacggggac gggtaaaact gtcacgctgc
     3001 aaaaactggc ggagtctcta tcggaaatcg gcgtgccggt gtttatggcc gatgtgaaag
     3061 gcgatctgac cggtatcgcg caggcaggaa cggcgtcgga aaaactgctc gcaaggctta
     3121 aaaatatcgg cgtaaatgac tggcaaccgc atgctaatcc ggtggtggtg tgggatatct
     3181 ttggcgagaa aggccatccg gtgcgggcga cggtttcgga tctggggccg ctgttgctgg
     3241 cgcggctgtt gaatctcaac gatgtgcaat ctggcgtgct gaatatcatc ttccgaattg
     3301 ctgacgatca ggggctgtta ctgctcgact ttaaagatct gcgggcgatt acccagtaca
     3361 tcggcgataa cgccaaatct ttccagaatc agtacggaaa tatcagtagc gcatcggttg
     3421 gtgccatcca gcgcgggctg ttgtcgctgg aacagcaagg cgcagcacac ttctttggtg
     3481 agccgatgct ggatatcaaa gacttgatgc gcaccgatgc caacggtaaa ggcgttatta
     3541 atatcctcag cgccgagaag ctttatcaga tgccgaaact gtacgccgcc agcctgctgt
     3601 ggatgatttc agagttgtat gaacaattgc cggaagcggg cgatctggag aagccaaaac
     3661 tggtgttttt cttcgacgaa gcacatctgc tgtttaacga tgcaccgcag gtactgctgg
     3721 ataagattga gcaggtgata aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt
     3781 cgcaaaaccc gtctgatatt ccggataatg tgctcggcca gctcggtaat cgcgttcagc
     3841 acgctttgcg ggcttttacg ccaaaagatc agaaagcggt aaaagctgcg gcgcaaacca
     3901 tgcgggccaa tccggcgttt gataccgaaa aggcaatcca ggaactgggg accggcgagg
     3961 cgttaatctc gtttctcgat gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga
     4021 ttgcgccttg ttcgcggatg ggaccggtga cggaagatga gcgcaatggc ttgattaacc
     4081 actccccggt gtatggcaag tacgaggatg acgtggaccg ggaatccgcc tatgagatgt
     4141 tgcaaaaagg cgtacaggcg agtattgaac agcaaaataa tcctccagcg aaagggaaag
     4201 agatagcggt agatgacggc attcttggtg gattgaagga tattttgttc ggcactaccg
     4261 gaccacgcgg cgggaagaaa gatggtgtgg tgcagacaat ggccaaaagc gccgctcgcc
     4321 aggtgacgaa tcagattgta cgcgggatgt tggggagttt gctgggaggg agaagaaggt
     4381 aagtcaggcg cgatctgtcc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg
     4441 atcgaactcg tatttgactt tatcaacaat gtgaaaggga gctttcgctc ccttgcttgg
     4501 ttacgatttt ctcattaaca gccacaggct gattaagaag aagctggcgc ttggcaacag
     4561 tgcgccgata atcggcggga tgccgtaaac caacgtcagc gggccgaaga tctggtccag
     4621 tacgtagaag acaaaaccga agctgatacc ggtgaccaca cgcacgccca tcggtacgct
     4681 acgcagtggg ccaaagatga acgacagcgc catcagcatc atcaccgcca cggatagcgg
     4741 ctggaagatt ttgctccaca tattgagctg ataacgtccg gcatcctgac cgctcgactt
     4801 cagatacttc acgtagttgt gcaaaccgct gattgagagt gcatccgggt ccagcgccac
     4861 cacgcccagt ttgtctggcg tgaggttggt tttccaggtg ccgctcaccg tctgcgaacc
     4921 ggtgatctgt ttcggattgg tcagatcaga ttcatcaacc tgcgacagac gccagacttt
     4981 atgttccggg tcaaacttcg cagtagcggc atagcgtacg gattgcagac gacgattttc
     5041 gttaaaggca taaatgctga tgccacctaa ctcttcgtca cctttaaccc gctcaatgta
     5101 gacgaagttg ttgccatctt tcgcccataa gccttgctgg gtagagagca acgagccgcc
     5161 gtacatcgcc tgcgcacggt agttacgcgc catctgctcg ccctgcggcg cgacccattc
     5221 gccaatcgcc atcgtcagca agaccagcgg aatggcggtt ttcatcaccg acagcgccac
     5281 ctgcatacgg gtaaaaccag aagcctgcat caccaccagt tcgctgcgct gcgccagcat
     5341 cccaagacca agcaacgccc caagcagagc cgccatcggg aagaagattt gcacatcttt
     5401 cggcacgctc agcaaggtat acattcctgc gcctaacgcg tcgtaactcc cctgcccggc
     5461 ttttttcaac tgatcgacaa atttgataat gcccgacagc gacaccagca tgaacagcgt
     5521 catcatgatg gtggtgaaaa tagttttacc gatatagcgg tcaagtacgc caaaaggttg
     5581 catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc
     5641 caaaggttga gaacaatcgc caaagccaga taaatcaggt taacggtcca catccacagc
     5701 gtcgggtcca gcttaccttt accgccgttc gatttcaggg aggtctggat caggaagaaa
     5761 agtagataca gcagcatggc tggcagcatc gacagtacgc gtccctgacg tgggttaacc
     5821 acgctcagcg gtacgaccat aagtgccatc ataaacacgg tgaataccaa cgtgatacgc
     5881 cagttcagtt ctgcgcgagc acgatcggtg tcagtgttcc acaatgtgca catgtccatc
     5941 tggtcggtat cgttcgggtc gagcgccacc gcctggtgac caatgatcgc ctgataatcc
     6001 tggaaatccg taatgcggaa atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg
     6061 ttgagagtga cgacctggga gccgtcgcgc agctgggtta aatgtccgga atcggccacc
     6121 accacagaag gacgtgcatt accttttggt cgaatttgtg cgaggaacac atctttgaaa
     6181 tcgctgccgt caacgctttc gatgaacagc accgagctgc cattagtcgc ttgctggaat
     6241 tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg cttctgctaa cacttcatcc
     6301 tgatgacgcg atgaccacgg tcccgcccac atcacgttaa ccgccgcgac gattgccgtg
     6361 aataccgcaa ggatcattgc cgctttcacc agaaccgctt tgctcaggcc gcaggcatgc
     6421 attaccgtaa tttcactttc ggtatacagt ttgcccagcg tcatcagcag cccgaggaac
     6481 aggcttaatg gcaggataag ctgcgccatt tccggcacgc ccaacccgag aagggagagc
     6541 accagattcg ccggaatatc gccgtcaacc gctgcgccga ggatcctcac taacttttga
     6601 cagaagaaga tcaaaagcaa gatgaagagt atcgccagct ggcttttgag cgtctcccgc
     6661 accagatatc ttatgattat cactttaaat acgcccgtaa aaactcgtct tttgcaggat
     6721 tttagcttgt ttcatggctt aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta
     6781 agataattat actcaacgga ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa
     6841 taacgttaag attaacacga agtcatcgca acagcggaca tgagttacga aagcttgcaa
     6901 ttctatctgt agccaccgcc gttgtcttta agattcagga gcgtagtgca tggagtttag
     6961 tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga
     7021 accacgtcgc ctttctccga ttgcagaaca gctcgataaa atcagcgatg ggtacatcag
     7081 cgccctgcta cgtcggggcg aactggaagg aaaaccgggg cagacattgt tgctgcacca
     7141 tgttccgaat gtactttccg agcgaattct ccttattggt tgcggcaaag aacgtgagct
     7201 ggatgagcgt cagtacaagc aggttattca gaaaaccatt aatacgctga atgatactgg
     7261 ctcaatggaa gcggtctgct ttctgactga actgcacgtt aaaggccgta acaactactg
     7321 gaaagtgcgt caggctgtcg agacggcaaa agaaacgctc tacagtttcg atcagctgaa
     7381 aacgaacaag agcgaaccgc gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg
     7441 ccgtgaactg accagcggtg agcgcgcgat ccagcacggt ctggcgattg ccgccgggat
     7501 taaagcagca aaggatcttg gcaatatgcc gccgaatatc tgtaacgccg cttacctcgc
     7561 ttcacaagcg cgccagctgg ctgacagcta cagcaagaat gttatcaccc gcgttatcgg
     7621 cgaacagcag atgaaagagc tggggatgca ttcctatctg gcggtcggtc agggttcgca
     7681 gaacgaatcg ctgatgtcgg tgattgagta caaaggcaac gcgtcggaag atgcacgccc
     7741 aatcgtgctg gtgggtaaag gtttaacctt cgactccggc ggtatctcga tcaagccttc
     7801 agaaggcatg gatgagatga agtacgatat gtgcggtgcg gcagcggttt acggtgtgat
     7861 gcgtatggtc gcggagctac aactgccgat taacgttatc ggcgtgttgg caggctgcga
     7921 aaacatgcct ggcggacgag cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca
     7981 aaccgttgaa gtgctgaata ctgacgctga aggccgcctg gtactgtgcg acgtgttaac
     8041 ttacgttgag cgttttgagc cggaagcggt gattgacgtg gcgacgctga ccggtgcctg
     8101 cgtgatcgcg ctgggtcatc acattaccgg tctgatggcg aaccataatc cgctggccca
     8161 tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca tggcgcttac cgctgggtga
     8221 cgagtatcag gaacagctgg agtccaattt tgccgatatg gcgaacattg gcggtcgtcc
     8281 tggtggggcg attaccgcag gttgcttcct gtcgcgcttt acccgtaagt acaactgggc
     8341 gcacctggat atcgccggaa ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg
     8401 tccggtagcg ttgctggcac agttcctgtt gaaccgcgct gggtttaacg gcgaagagta
     8461 attgcgtcag gcaaggctgt tattgccgga tgcggcgtga acgccttatc cgacctacac
     8521 agcactgaac tcgtagatac tgataagacg cgcaagcgtc gcatcaggca atgctgttat
     8581 tgtcggatgc ggcatgaacg ccttatccga cctacacagc accgaactcg taggcctgat
     8641 aagacacaac agcgtcgcat caggcaatgc tgttattgtc ggatgcggcg tgagcgcctt
     8701 atccgaccta cacacagcac tgaactcgta ggcctgataa gacacaacag cgtcgcatca
     8761 ggcgctgcgg tgtatacctg atgcgtattt aaatccacca caagaagccc catttatgaa
     8821 aaacgcgacg ttctaccttc tggacaatga caccaccgtc gatggcttaa gcgccgttga
     8881 gcaactggtg tgtgaaattg ccgcagaacg ttggcgcagc ggtaagcgcg tgctcatcgc
     8941 ctgtgaagat gaaaagcagg cttaccggct ggatgaagcc ctgtgggcgc gtccggcaga
     9001 aagctttgtt ccgcataatt tagcgggaga aggaccgcgt ggcggtgcgc cggtggagat
     9061 cgcctggccg caaaaacgca gcagcagccc gcgcgatata ttgattagtc tgcgaacatg
     9121 ctttgcagat tttgccaccg ctttcacaga agtggtagac ttcgttcctt atgaagattc
     9181 tctgaaacaa ctggcgcgcg aacgctataa agcctaccgc gtggctggtt tcaacctgaa
     9241 tacggcaacc tggaaataat ggaaaagaca tataacccac aagatatcga acagccgctt
     9301 tacgagcact gggaaaagca gggctacttt aagcctaatg gcgatgaaag ccaggaaagt
     9361 ttctgcatca tgatcccgcc gccgaacgtc accggcagtt tgcatatggg tcacgccttc
     9421 cagcaaacca tcatggatac catgatccgc tatcagcgca tgcagggcaa aaacaccctg
     9481 tggcaggtcg gtactgacca cgccgggatc gctacccaga tggtcgttga gcgcaagatt
     9541 gccgcagaag aaggtaaaac ccgtcacgac tacggccgcg aagctttcat cgacaaaatc
     9601 tgggaatgga aagcggaatc cggcggcacg attacccgtc agatgcgccg tctcggcaac
     9661 tccgtcgact gggagcgtga acgcttcacg atggacgaag gcctgtccaa tgcggtgaaa
     9721 gaagttttcg ttcgtctgta taaagaagac ctgatttacc gtggcaaacg cctggtaaac
     9781 tgggacccga aactgcgcac cgctatctct gacctggaag tggaaaaccg cgaatcgaaa
     9841 ggttcgatgt ggcacatccg ctatccgctg gctgacggtg cgaaaaccgc agacggtaaa
     9901 gattacctgg tggttgcgac tacccgtccg gaaaccctgc tgggcgatac cggtgtagcc
     9961 gttaacccgg aagatccgcg ttacaaagac ctgattggca aatatgtcat tctaccgctg
    10021 gttaaccgtc gtattccgat cgttggcgac gaacacgccg acatggaaaa aggcaccggc
    10081 tgcgtgaaga tcaccccggc gcacgacttt aacgactatg aagtcggtaa acgtcacgcc
    10141 ctgccgatga tcaacatcct gacctttgac ggcgatatcc gtgaaagcgc ccaggtgttc
    10201 gataccaaag gtaacgaatc tgacgtttat tccagcgaga tcccggcaga attccagaaa
    10261 ctggagcgtt ttgccgcacg taaagcagtc gttgctgcgg ttgacgcact cggcctgctg
    10321 gaagaaatta aaccgcacga cctgaccgtt ccttacggcg accgtggcgg cgtagttatc
    10381 gaaccaatgc tgaccgacca gtggtacgtg cgtgccgatg tactggcgaa accggcggtt
    10441 gaagcggttg agaacggcga cattcagttc gtaccgaagc agtacgaaaa catgtatttc
    10501 tcctggatgc gcgatattca ggactggtgt atctctcgtc agctgtggtg gggtcaccgt
    10561 atcccggcat ggtatgacga agcgggtaat gtttatgttg gccgcaacga agaagaagtg
    10621 cgcaaggaaa ataacctcgg tgccgacgtt gccctgcgtc aggacgaaga cgttctcgac
    10681 acctggttct cttctgcgct gtggaccttc tctacccttg gctggccgga aaacaccgac
    10741 gccctgcgtc agttccaccc aaccagcgtg atggtatccg gcttcgacat catcttcttc
    10801 tggattgccc gcatgatcat gatgaccatg cacttcatca aagatgaaaa tggcaaaccg
    10861 caggtgccgt tccacactgt ttacatgacc ggtctgattc gtgacgacga aggccagaaa
    10921 atgtccaaat ccaagggtaa cgttatcgac ccgctggata tggttgacgg tatttcgctg
    10981 ccagaactgc tggaaaaacg taccggcaat atgatgcagc cgcagctggc ggacaaaatc
    11041 cgtaagcgca ccgagaaaca gttcccgaac ggcattgagc cgcacggcac cgatgccctg
    11101 cgcttcaccc tggcggcgct ggcgtctacc ggtcgtgaca tcaactggga tatgaagcgc
    11161 ctggaaggtt accgtaactt ctgtaacaag ctgtggaacg ccagccgctt tgtgctgatg
    11221 aacacagaag gtcaggattg cggctttaac ggcggcgaaa tgacactgtc gctggcggac
    11281 cgctggattc tggcagagtt caaccagacc atcaaagcgt accgcgaagc gctggacagc
    11341 ttccgcttcg atatcgctgc aggcattctg tatgagttca cctggaacca gttctgtgac
    11401 tggtatctgg agctgaccaa gccggtaatg aacggtggca ccgaagccga actgcgcggt
    11461 actcgccata cgctggtgac tgtactggaa ggtctgctgc gcctcgcgca tccgatcatt
    11521 ccgttcatca ccgaaaccat ctggcagcgt gtgaaagtac tttgcggtat cactgccgac
    11581 accatcatgc tgcagccgtt cccgcagtac gatgcatctc aggttgatga agccgcactg
    11641 gccgacaccg aatggctgaa acaggcgatc gttgcggtac gtaacatccg tgcagaaatg
    11701 aacatcgcgc cgggcaaacc gctggagctg ctgctgcgtg gttgcagcgc ggatgcagaa
    11761 cgtcgcgtaa atgaaaaccg tggcttcctg caaaccctgg cgcgtctgga aagcatcacc
    11821 gtgctgcctg ccgatgacaa aggtccggtt tccgttacga agatcatcga cggcgcagag
    11881 ctgctgatcc cgatggctgg cctcatcaac aaagaagatg agctggcgcg tctggcgaaa
    11941 gaagtggcga agatcgaagg cgaaatcagc cgtatcgaga acaagctggc gaatgaaggc
    12001 tttgtcgccc gcgcaccgga agcggtcatc gcgaaagagc gtgagaagct ggaaggctat
    12061 gcggaagcga aagcgaagct gattgaacag caggctgtta tcgccgcgct gtaatcaaca
    12121 ccgtgatgaa aacgaaggcc ggagaatgct ccggcctttt ttatctctta cagaaatggt
    12181 aaagaaggca taattccccg tgaaatactg gctaagaagc ctttatctgg ttgttcttct
    12241 ttattgatta aaggaaggtc atccaaatcg cccagcaaat acgtattttt tgcctgccag
    12301 tcaatcatcc atattttcag cagtggataa gccagaccgc ccgtaatccc ggatatcact
    12361 accaacgcac acatgcgata aagcataccg tggtaagtta aagttgagcg aaaacgaata
    12421 cgcccatcat tcagtgacag gttgctcata aaatgatttc gcaacgacac cgttaaataa
    12481 ctggtgctaa cagcaatccc aaaataatag attaactgcg cgattatcat tttacgttgc
    12541 atttccataa attgctgtaa attctcaata tcatcatttg catacacaga actatcatac
    12601 gcatttaata tttgatcgaa gataatataa ccagcaacag cgagaaaagg taataaagct
    12661 aaaaatgcga ggatggcata ttttatacaa tacgcagtat ctaatttcac cttgaaacga
    12721 tgtataccga aactggtatt gctccagaga aaactcatta ccaggctata taaagtacca
    12781 ttaaaaatac caatggaaac aataccaact attgccatca gaaccacaga tataataaca
    12841 ctacttgaac tattggcatg tagcatcttt gtcgagataa agaaaacagt ccccatccca
    12901 atggccatta aaatcggcaa gaaaaaggtt actcaccaga accctttcat agagcaatta
    12961 aaactaaatc ttacgccgtt gagactagtc atcaaggcct gataacgcag tccttttgct
    13021 gccataaata taagcaaaac caacagtgac aaagtcaaaa cacagccaat aagcggcata
    13081 tctgctgaca ctgtcattaa gattgcgaaa tagaaaaaaa caaaaacaag acaactaaca
    13141 aaaacattcc caccggtaat cccataagaa aatcgttgtc cgttaacctc catattagca
    13201 tagagataac gcttacattt cattaatgcc catggtaaat agatacctag cgttataatc
    13261 gttaacaaca cattcaccac acaaataaga aaatatctct cacctgtacc atgaaaaaca
    13321 aacgaatggg acggaacatc catttcatta ataacttgag ccataaaaat tccctgaatt
    13381 aaaaaatcca t
//
LOCUS       DAZFJF010000116        13262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFJF010000116 DAZFJF010000000
VERSION     DAZFJF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.116"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            445..1101
                     /gene="pphA"
                     /locus_tag="ACHKAU_003842"
     CDS             445..1101
                     /gene="pphA"
                     /locus_tag="ACHKAU_003842"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5532820.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1102..1293)
                     /gene="yebW"
                     /locus_tag="ACHKAU_003843"
     CDS             complement(1102..1293)
                     /gene="yebW"
                     /locus_tag="ACHKAU_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO5532821.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1398..1634)
                     /gene="yebV"
                     /locus_tag="ACHKAU_003844"
     CDS             complement(1398..1634)
                     /gene="yebV"
                     /locus_tag="ACHKAU_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO5532822.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1752..3191)
                     /gene="rsmF"
                     /locus_tag="ACHKAU_003845"
     CDS             complement(1752..3191)
                     /gene="rsmF"
                     /locus_tag="ACHKAU_003845"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO5532823.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3271..5904)
                     /gene="yebT"
                     /locus_tag="ACHKAU_003846"
     CDS             complement(3271..5904)
                     /gene="yebT"
                     /locus_tag="ACHKAU_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO5532824.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5873..7156)
                     /gene="yebS"
                     /locus_tag="ACHKAU_003847"
     CDS             complement(5873..7156)
                     /gene="yebS"
                     /locus_tag="ACHKAU_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO5532825.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7286..7783
                     /gene="msrC"
                     /locus_tag="ACHKAU_003848"
     CDS             7286..7783
                     /gene="msrC"
                     /locus_tag="ACHKAU_003848"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO5532826.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7880..8578
                     /gene="proQ"
                     /locus_tag="ACHKAU_003849"
     CDS             7880..8578
                     /gene="proQ"
                     /locus_tag="ACHKAU_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO5532827.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8598..10646
                     /gene="prc"
                     /locus_tag="ACHKAU_003850"
     CDS             8598..10646
                     /gene="prc"
                     /locus_tag="ACHKAU_003850"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO5532828.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     ALEIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            10838..11719
                     /gene="htpX"
                     /locus_tag="ACHKAU_003851"
     CDS             10838..11719
                     /gene="htpX"
                     /locus_tag="ACHKAU_003851"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO5532829.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11765..13138)
                     /gene="yebQ"
                     /locus_tag="ACHKAU_003852"
     CDS             complement(11765..13138)
                     /gene="yebQ"
                     /locus_tag="ACHKAU_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5532830.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT         3479 a         3275 c         3424 g         3084 t
ORIGIN      
        1 aagaacgtgc cacggctggc tgatggacgt tcgatagcgc gagtttgaat gaaaatcagc
       61 cggagatgat tttacataat tgctacggaa ttattcaata caggaattgc ttgtgtatgc
      121 atggattgac ctgaaatatt cccgaaaatt tctctaaaaa actcgaaaaa aatggtaact
      181 aattgaatgt attaatatgt aatagtacgt gttagagatt aaaagatgag cataaattta
      241 tttaacacat taattctaaa agattttgtt gtttgttgac gaaaacagga atcgtgttcg
      301 gtctcttttt atctgttaaa agccagaagc atttccttcg ctgactttat agtcaaccat
      361 aacacacact ctactgtctg agtccagcgt tttttaacat tcttgttaag attatgtgat
      421 ctttagcgcg ggaggaaaat attgatgaaa cagcctgcgc ccgtttatca gagaattgcg
      481 ggtcatcaat ggcgacatat ctggctttct ggcgatatac acggttgtct tgagcagttg
      541 cgccgcaaat tatggcattg tcgttttgat ccgtggcgag atttacttat ctcagtagga
      601 gacgttatcg atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg
      661 gtttgtgcgg taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag
      721 atgtctttgt ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag
      781 aaacaagcga aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac
      841 agtcgtaccg gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg
      901 caaaaggacg ttgatttgca tcaggtcttg tggagccgct cgcgattagg tgaacgccaa
      961 aaagggcagg gaattacagg tgctgatcat ttctggtttg gtcatacacc gttgcgacat
     1021 cgcgtggata ttggcaacct gcattatatt gataccggtg ctgtctttgg gggcgaactg
     1081 actcttgtgc aattgcaata attaaaaatc accatactcc tgtgctggtc gccagaaacc
     1141 atctataaaa tcctcaatcg gaaaacaacc gccatggcgg atccgttgat cgctcataga
     1201 ataaagacac tgctgttccg tgttgtagac atccacaaca atatcttcac aaccgccatc
     1261 caggtagcaa acaaaaagta ccagcgcgaa catttcatcc ccgaagtgtg gtgccgtacc
     1321 gttaagttta ggagagattt tacaacgggg gaataaccag gacaaataac ccgccatgcc
     1381 gacgggttct ttttggatca ggcaagacgc ataatccagg catcagattg ttgatcgtaa
     1441 cgggtacggt aaaggacaga gtgttcgcca tttagcaggg cagggatact ggtttcagca
     1501 tcccaggcat cgagttgcat acataaatcg gggtcagatt tacaaggaat ggttaacgtt
     1561 cgatcacccg gcgattcacc gtgtaattcg ccgtcgtcga tttcaaaagc gcctatgcgc
     1621 acactggttt tcatcttcgt gctctccatt tagctgccga ttgtggtacg accagtccga
     1681 tcgcaccatt tttcagccta gttgaggcag ccgaatgtgc cagtcaaaaa gtgcgctttt
     1741 tttgtgagct gtcaggcgtt accggtaaaa agtttcccat cgcgcaccag ttcacgcgga
     1801 tagctgtttt tcaatcgcga accaatccgt ttggctaaac caatcggttg atgctggaaa
     1861 gtaaccaata catcatccgc cactggcgcg gcttgcgggt aaacatcgcg cccgcgatac
     1921 cactcctccg cttcctgcgg tgtcagctcg aatgcattca tattatcggg tgaggcaaga
     1981 gcaataaccg cttcatgctg ccagcgataa cctttgttgt gcgtctcggc aagtttaatc
     2041 cccaaccgag aaaatcggac tttaccgatc agggcttcaa tgcccaccgg gaacaaccac
     2101 agttctttgt cacgctgcca gaggcgcagg ttttcatccc agtttaagcc aacacctgca
     2161 gtcgcctgac gaatttgtcc agcttcgcga tctttcaccg ggctgaacgg aaaattaccg
     2221 actttgtatt tgggggcggg taaggcggga atcgcctgag ttttacgcag acgagcaacg
     2281 aagaagcctt cgcagtcgta aatttgtggg aaaacatgca aaaagccttc ttcggtcagc
     2341 gctttgtttg caccagggaa gagatcgcca agtggtaaaa actctactgc gtcggggtaa
     2401 gtctctttca gccacaggca aacggcttcg ttttcttcct ggtttaaggt acaggtcgag
     2461 taaaccagcg taccaccagg acgtaatgca tgaaaggcgc tgtcgataag ctcccgttgt
     2521 gtagctgcga tttcctgatt gctttctggt gaccagtttt ttagcgcatc gggatcttta
     2581 cgcaccacgc cttcgccaga gcagggagcg tccagcaaaa tggcatcgaa catttctggc
     2641 actgccgcac caaacacgcg gccatcaaaa tgtgtgagcg caacattact gatgccacag
     2701 cggctgatat tggcatgtaa cacttttacc cgactggcgg aaaactcatt ggcaaggatt
     2761 gccccttcgt tattcatccg cgcggcaatt tgcgtcgttt tggagcctgg cgcagctgcg
     2821 acatccatca cccgctgtgg tgcattatcg tcagcaaaca aggcggcaac gggcaacatt
     2881 gaactggctt cctgaatata aaacaggccg cttaaatgct cggcggtact acccaatggc
     2941 aatgcatctt cattgtcgcg ttcaatccag aaaccttctt cacaccacgg aattggcgta
     3001 agcgtccagc cataaggagc ggttaattgc aggaaatcag caacggagat tttcagcgta
     3061 ttaacgcgaa tgctgcggcg caacgggcgc tgacaggcgg caagaaaatc atcaaatgag
     3121 agcgtcgaag gcatcgcttc gcgcatttgt gtcagaaagg cgtccgggaa ataaacggtg
     3181 tgttgggcca cgagcatgta ccactggcaa aaaaacaggc gcgcagttta gcataaacgc
     3241 tccggcgcag gcacgccgga gcagtgggca ttatttggga agcgcagttc cccattcacg
     3301 ccactctttc ggttcacttt cctgcaacag gaagtgtttg ccttcctggg ctttcggtgc
     3361 cagtggcgta cccggaggcg tggcgaaggc gatgccgcca cggataaact ggttaaaggt
     3421 gccggttttc actacgccgc ccgtcagacc aaagtccaga ctgtaacccg atgccaacca
     3481 gaagacggaa ttgttacgca ccaggtgttg atagcgttta ctgatgcgca tcgcaatcat
     3541 cacgcgatct gacaatgtcc ccagcgtcat tcctgtaacc gtaccgactt ccagaccacg
     3601 gaacagcaca ggcgtaccaa tacctaacga accggcttcc ggcgcttcaa caataatgct
     3661 taagccatcc aggtaacgcg aatcagtaat ggtggcctct tgtaattcaa agtcgcggcg
     3721 aggattgccc cggcctggtt cgacgttgat atacggctgg aggatagtat ctagatgctc
     3781 aacgcccgcg gctgaaattt gcggtgtgac caccgagaag cgcgtaccgc cgcgggcgaa
     3841 ggtctggaca tactccggat agagcaccgc ctttgcctgt acttcattgc gcgcggtaat
     3901 cagatccagc gtctggattt gcccgatatc aataccgaga tagcgaattg gcatcccgac
     3961 cgccagtttt ccggcatcga aagcgtgaag cgtaatctgc ccaccaaccg cacgggccgc
     4021 tgtttcggaa gcatacagaa ttcgtttgtc gcctttacgc tgactggcgc tggcaccgct
     4081 gaggttatcg aagctaatgg ctccctttaa tgctctggag agcggggatg cctgtacggt
     4141 caggccacta ccattcagct gaactttcgc cccgccttct gcccagaaca cgctattgct
     4201 ggtcagaagg ttgcgatact ccggcttaat atgcagatcg atatcaaacg cgttagctcg
     4261 cggacggacg gtaataactt caccaacttc aaatttacgg tagagcacta ccgatcctgc
     4321 ctgcacatcc ggcagcgtct ctgcactcaa actcacggtt gtggtgggta aatcgctaag
     4381 gctgttctcc agcgcttttt ccagattggc atacagtgga tagctggctt tcatctcgcc
     4441 tttatcgccc ggcagaatac gtatcccgcc gttaatccat tctgaggcgc tggcaccgag
     4501 aaactcaacg ccatccagcc ccaccttcac gtcgacacgg ctgttgacga caaatttgct
     4561 atcgcctttt accagttctc gatgctgagg ctcgatggcg acggtaaagg tgacgccttt
     4621 gctggtgagt ttacgatcga taacctggcc tacctgcacg ccgtgaagaa tgagcggctg
     4681 accagcatca ataccgtaac tttccggcgc ggtcagggtc agcgtcagaa catcaggttc
     4741 atgcagcagt gctttttcgc ctggcacaac aacgaactct ttgcgtggct cgccatcgcc
     4801 gggtaccaac tcgaaggttt tgccagtcag cagggcgctg agattggcat cgctaaggga
     4861 taatttcgga ttacgtaatt cgatgcgggt attttcacga agcagggtaa cgacgctggg
     4921 atcaactgtc atttccccag tgactttacc accaggattt aaatccagtt tagttagctg
     4981 tccgacttcc agcccctgat acattaacgg cgtcgagtcg gcggtcaatc cggccccact
     5041 cggcagttcc agttttatta ttacgccacg ctggctgtgg gccagatctt catacagacc
     5101 aaaggtatct tccgcctcgg caggtttcga ctcttcaggt gaatcgaagg caatcgcacc
     5161 gttaaccagt gccgccagac tttccagttt caccttcgcg ccactgacac tgacgttggc
     5221 atcaacgccg gaaacgttcc agaaacggct accttttttc accagatcgg taaaacgccg
     5281 ctcgatcagg acatcaatca ccacgccttg cttgttggga ttgatggcat agtcgtagac
     5341 ttttcccacc gggatcttgc ggaaatagac caatgaaccg ctgttcagcg aaccgagatc
     5401 gggagcttgc aggtggatca tcagatcgcc gttatccagc cgatacttcg gttgggtatc
     5461 gagtgcgaca aagtgatcct gctctttacc tttacccggc atcatgccga tatagttccc
     5521 accgacgagg gcgtccagcc cggagacacc tgccaacgat gcttttggcg tcaccagcca
     5581 gaattgagtc tcttcgcgca gcgcatcttt catatcggac ttgatgctga ccttgacttc
     5641 aatcttacga agatcgtcgc tgaggctgat atcctgcact gttccgactt caacgccctg
     5701 ataacgaaca ggcgtacggc ccggaacaat accatccgcc gacataaagt cgatggtgac
     5761 ggtattaccc cggtcctgat aactgtccca aatcagccaa ccggcaatca ttagcgcgat
     5821 gaaaggcagc agccagaaag gtgagatacg gcgtttattt ttaatctgcg cttcagtcgt
     5881 cgaagcgggc gtttcctgac tcatgtgcat cccaaagtaa gcggctgtcc agccattcca
     5941 cagcaagaat agtcaaaatt acggctgcgc caaaataaaa cgcagccggt cccatagtaa
     6001 aagcgaggat ctgatcgcga ttaatcagcg acatggttaa agatatgaca aacaggtcga
     6061 gcatcgacca gcgtccaatc caggtcacca tccgcagtaa cagaatgcgt gtgcgtaaac
     6121 cttgctggca tttaaaatga atgctcaaca gtaaagtgaa catgacgatc actttagtaa
     6181 acggtaccag aatactggcg ataaacacga ttcccgcaac cgcaatgttg ctacttgcca
     6241 gcgacataat tccggaaaga attgtatctt cctgccgtcc tccgttcaga taaatgatag
     6301 aaataggcaa taggttggct ggtaacaaca aaacgattga cgctaacagc gccgcccagc
     6361 atttttgcag actatgacgg cgacgcaggc gtagcgggat atggcaacgc gggcagcgac
     6421 cacgctgatc aggatagccg gtaaaatggc acccaagaca gacacgaagt ttctcgtccc
     6481 tacgcgtagc ggggcgctgc ggataatatc gctcccacag ctcctcaaca ttaagatgtg
     6541 acaacgtcac cgtcgttaaa atcaccaacg ccacaaaaga gaacaagccg acaccagcct
     6601 ggatatgggc ataatcctgt acctttatag aagcaacgcc aatgccgacc aggtagatat
     6661 ccagcattac ccactcttta agacgctcaa gcatcagcag taccggacgt aaattcattc
     6721 ccagtcggtt accaaaccat aaatatgcta tggaggacac cagaatgagg ggggcaccaa
     6781 taacgcagaa aaagaccatc gcccccgtta tcgtatcgcc ctgtttggtc atttgccaga
     6841 tgccttgcat gacgttggcg tcgatacgaa tacctaacag ccagatatgc aacagcggtt
     6901 cgccccaggc aaacggcatc agcaacagca tggtgaaagc cattgccgcc aggcgcgtta
     6961 gcgaccagtc gcgcccgtcg cgaatttttg cctgacagcg cggacaatag gcactctgat
     7021 gagaatttat ctcgggcagg ctaaacagca tgtcacattg cgggcaacgt tggtaatcac
     7081 cacgcggcag ttcctcgccg attgccctca ctgttatctt ttttgtcggc gtaatttgtg
     7141 gtgtgttaag agccatgacg tgccaaatgt aataagaatt aacattaata ttaacccatg
     7201 attggaatga tcttgagcat taacgcattt aatgcttatt ttaataggct ggcagttcca
     7261 ggtaagacaa ccaagtgatt atataatgaa caaaacagaa ttttacgcgg atttaaatcg
     7321 cgactttaac gcgctgatgg cgggagaaac cagttttctg gcaacgcttg cgaacaccag
     7381 tgcgttgtta tacgagcgtc tcactgacgt aaactgggca ggtttttatt tgcttgagga
     7441 cgatacactg gtactaggac catttcaggg caaaattgcc tgtgtccgga tacccgtcgg
     7501 gcgcggggtg tgcggcactg cggttgcccg caatcaagtg cagcgtatcg aggatgttca
     7561 cgcgtttgac gggcatattg cctgtgatgc ggcgagtaat tctgaaattg ttctgccgct
     7621 ggtggtgaaa aatcagatta ttggtgttct tgacatcgat agtaccgtct tcggtcgctt
     7681 tacagacgag gacgagcaag gcttacgtca gcttgtggca cagcttgaaa aagtgcttgc
     7741 aacgacggat tacaaaaaat tctttgcgag cgtcgcagga taatcaacgg ataacgtagc
     7801 atttactggt agcgtcatta taatgacgcc tgttcatgcc tgcgcttgtt ggctacgtcc
     7861 gttgtaatca ggaaatttca tggaaaatca acctaagttg aatagcagta aagaagtaat
     7921 cgcgtttctg gccgaacgtt ttccccactg tttcagtgcg gaaggtgaag cgcgtccgct
     7981 gaaaatcggt atttttcagg atttggtcga tcgtgttgcc ggggaaatga acctgagcaa
     8041 aacgcaatta cgatccgctt tacgtctcta cacttcgagc tggcgttatc tttacggtgt
     8101 taaacccggc gcaacgcgtg tcgatcttga cggcaaccca tgcggtgagc tggacgagca
     8161 acatgtagag catgctcgca agcagcttga agaagcgaaa gcgcgtgttc aggcacagcg
     8221 tgctgaacag caagcgaaaa aacgcgaagc tgccgcagct gctggtgaga aagaagacgc
     8281 accgcgccgc gaacgcaagc cacgtccgac cacgccacgc cgcaaagaag gcgctgaacg
     8341 taaacctcgt gcgcaaaagc cggtagagaa agcgccaaaa acagtaaaag cacctcgcga
     8401 agaacagcac accccagttt ctgacatttc agctctgact gtcggacaag ccctgaaggt
     8461 gaaagcgggt caaaacgcga tggatgccac cgtattagaa atcaccaaag acggcgtccg
     8521 cgtccagctg aattcgggta tgtctttgat tgtgcgcgca gaacacctgg tgttctgaaa
     8581 cggaggccgg gccaggcatg aacatgtttt ttaggcttac cgcgttagct ggcctgcttg
     8641 caatagcagg ccagaccttc gctgtagaag atatcacgcg tgctgatcaa attccggtat
     8701 taaaggaaga gacgcagcat gcgacggtaa gtgagcgcgt aacgtcgcgc ttcacccgtt
     8761 ctcattatcg ccagttcgac ctcgatcagg cattttcggc caaaatcttt gaccgctacc
     8821 tgaatctgct cgattacagc cacaacgtgc tgctggcaag cgatgttgaa cagttcgcga
     8881 aaaagaaaac cgagttaggc gatgaactgc gttcaggcaa actcgacgtt ttctacgatc
     8941 tctacaatct ggcgcaaaag cgccgttttg agcgttacca gtacgctttg tcggtactgg
     9001 aaaagccgat ggatttcacc ggcaacgaca cttataacct tgaccgcagc aaagcgccct
     9061 ggccgaaaaa cgaggctgag ttgaacgcgc tgtgggacag taaagtcaaa ttcgacgagt
     9121 taagcctgaa gctggcagga aaaacggata aagaaattcg tgaaaccctg acacgccgct
     9181 acaaatttgc cattcgtcgt ctggcgcaaa ccaacagcga agatgttttc tcgctggcaa
     9241 tgacggcgtt tgcgcttgaa atcgacccgc ataccaacta tctttccccg cgtaataccg
     9301 aacagttcaa cactgaaatg agtttgtcgc tggaaggtat tggcgcagtg ctgcaaatgg
     9361 atgatgacta caccgttatc aattcgatgg tggcaggtgg tccggcagcg aagagtaaag
     9421 ctatcagcgt tggtgacaaa attgtcggtg ttggtcaaac aggcaagccg atggttgacg
     9481 tgattggctg gcgtcttgat gatgtggttg ccttaattaa agggccgaag ggcagtaaag
     9541 ttcgtctgga aattttacct gctggtaaag ggaccaagac ccgcactgta acattgaccc
     9601 gtgaacgtat tcgtctcgaa gaccgcgcgg ttaaaatgtc ggtgaagacc gtcggtaaag
     9661 agaaagtcgg cgtgctggat attccgggct tctatgtggg tttgacagac gatgtcaaag
     9721 ttcaactgca gaaactggaa aaacagaatg tcagcagtgt gatcatcgac ctgcgtagca
     9781 atggcggtgg ggcgttgacc gaagcggtat cgctctcagg tctgtttatt cctgcgggtc
     9841 ccattgttca ggtccgcgat aacaacggca aggttcgtga agacagcgat accgacgggc
     9901 aggtgttcta taaaggcccg ctggtggtac tggttgaccg tttcagtgct tcggcttcag
     9961 aaatctttgc cgcggcaatg caggattacg gtcgtgcgct ggttgtgggt gaaccgacgt
    10021 ttggtaaagg caccgttcag caataccgtt cattgaaccg tatttacgat cagatgttac
    10081 gtcctgaatg gccagcgctg ggttctgtgc agtacacgat ccagaaattc tatcgcgtta
    10141 acggcggcag tacgcaacgt aaaggcgtaa cgccagacat catcatgccg acgggtaatg
    10201 aagaaacgga aacgggtgag aaattcgaag ataacgcgct gccgtgggat agcattgatg
    10261 ccgcgactta tgtgaaatca ggcgatttaa cggcctttgg gccggagctg ctgaaggaac
    10321 ataatgcgcg tatcgcgaaa gatcctgagt tccagaacat tatgaaggat atcgcgcgct
    10381 tcaacgctat gaaggacaag cgcaatatcg tttctctgaa ctacgctgtg cgtgagaaag
    10441 agaataatga agatgatgcg acgcgtctgg cgcgtttgaa cgaacgcttt aaacgcgaag
    10501 gtaaaccgga gttgaagaag ctggatgatc taccgaaaga ttaccaggag ccggatcctt
    10561 atctggatga gacggtgaat atcgcactcg atctggcgaa gcttgaaaaa gccagacccg
    10621 cggaacaacc cgatcccgtc aagtaatatc aatcaggcac aagaaattgt gcctgatttt
    10681 ttaacagcgg caagatgccg taaatcagat gctacaaaat gtaaagttgt gtctttctgg
    10741 tgacttacgc actatccaga cttgaaaata gtcgcgtaac ccatacgatg tgggtatcgc
    10801 atattgcgtt ttgttaaact gaggtaaaaa gaaaattatg atgcgaatcg cgctcttcct
    10861 gctaacgaac ctggccgtaa tggtcgtttt cgggctggta ctgagcctga cagggataca
    10921 gtcgagcagc gttcaggggc tgatgatcat ggccttgctg ttcggttttg gtggttcctt
    10981 cgtttcgctt ctgatgtcca aatggatggc attacgatct gttggcgggg aagtgatcga
    11041 gcaaccgcgt aacgaaaggg aacgttggct ggtcaatact gtagcaaccc aggctcgtca
    11101 ggcagggatc gctatgccgc aagtggctat ctaccatgcg ccggacatca acgcttttgc
    11161 aaccggtgcg cgccgtgatg cctctctggt tgctgtcagc accggtttgc tgcagaacat
    11221 gagcccggat gaagccgagg cggtaattgc tcacgaaatc agtcacatcg ccaatggtga
    11281 tatggttacc atgacgctga ttcagggcgt ggtgaacacc ttcgttatct ttatttcccg
    11341 tattctggcg cagcttgccg cgggttttat gggcggaaat cgtgatgaag gtgaagagag
    11401 caacggcaac ccgctgatct actttgcggt tgcaacggtt ctggaactgg tgtttggtat
    11461 tctggcgagc attatcacca tgtggttctc gcgtcatcgt gaattccatg ctgatgccgg
    11521 ttcggcaaaa ctggttggtc gcgagaaaat gattgccgcg ctgcagcgcc tgaaaaccag
    11581 ctatgaaccg caagaagcaa ccagcatgat ggctttctgc attaacggta agtcgaaatc
    11641 gctcagtgag ttgttcatga ctcacccgcc gctggataaa cgaattgaag ctctgcgtac
    11701 gggtgaatac ctgaagtaat atgaagtaaa tactaaaagg cgcgtcctga tcgacgcgct
    11761 ttttttatgc cctggatcgt ggctgagtga tacgtaaacc actcacacag gcagcaatca
    11821 ctgccagaat agccgcagcc atcagcgaaa cgtgcgtacc attatcaccg aactgattta
    11881 gcatcagcgc caccagcgcc gcgccactac tctgacccag tagacgagcc gttcctaaca
    11941 tgccactggc cccaccgcta cgttcgcgcg gagcggaggt aataatggtg tggttattgg
    12001 gtgactggaa taagccaaat ccagcgccac ataagatcat cggccagata atattgatat
    12061 ccgcaggtga cgcgggcagc agaaccaggg aaaaaagccc cgcagccatg atgaacaacc
    12121 ctaaagcacc cagcaatcct gcatgtacgc gttcaatcaa atagcctgcc agcggagcca
    12181 tcaccatcgt tgctaacggc cacggtgtca gaagtaaacc tgtttcgact tcactacgcc
    12241 cgagcacggt ttgcaggtaa aagggcaggg aaaccattgc cagcatttgt gcgcagaaag
    12301 agcaaacaga tgtgcaaata gaaagtgaaa acagcgggat acgcagtaaa tccaccggta
    12361 gcagcggtac gggaagagaa agctggcggc gaataaagaa aataccaaca acaaccatta
    12421 ccaccagttc cgcagcaatt aacgtcagcg attgcccctg agcgaaacca ctcaacgcag
    12481 tgataagcag gccgaaggtt aacgcgttca tcacggcgct gggcaggtcg aaacggggtt
    12541 tactggcgcg agaaccattg ggtggcagaa aacgcatcgc cagaagcagg gcgataatac
    12601 ctaacggtac gttgattaaa aataaccatt tccaggatgc gatggagagg attgctgcag
    12661 caattgtcgg cccggcagca gaagaaacgg caacaataaa cgagtttatg cccatccctc
    12721 tccccagaaa acgttgtgga tagatcaggc ggataagtgc ggtattaacg ctcatcaacg
    12781 ctgcaccgcc gaaaccttgt atgacacgcg caagggtgag catttgcagc gaatcagaaa
    12841 gggcgcagaa cagtgaagac aacagaaaaa cgaccagacc gcatttataa atacgtcgat
    12901 agccaaacat atcgcccaga aacgaaaacg agagcaggga gatgacaatg gcgatttgat
    12961 aggcgttcac tacccagatg gatctggctg gcgtggcatg aaggtccgtg gcgattgttg
    13021 gcagggcgac gttggcgatt gcgccgtcaa ggacagccat cgaaatgcca atcacaatgg
    13081 ttaatatcgc accgtatcgc tggggcaatg gcaggccgtc ggcctgaact tttggcatag
    13141 gaattttata tctttggtga ataatcaaca ggggtatgat tctagcactg atattcacgg
    13201 tggatgtcgc agaattgtaa ctaaccggca aaagatggat tgcgaccacc ctggtgcgaa
    13261 at
//
LOCUS       DAZFJF010000117        13113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFJF010000117 DAZFJF010000000
VERSION     DAZFJF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.117"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(137..1561)
                     /gene="patD"
                     /locus_tag="ACHKAU_003853"
     CDS             complement(137..1561)
                     /gene="patD"
                     /locus_tag="ACHKAU_003853"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO5532831.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1583..2377)
                     /gene="ydcV"
                     /locus_tag="ACHKAU_003854"
     CDS             complement(1583..2377)
                     /gene="ydcV"
                     /locus_tag="ACHKAU_003854"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5532832.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2367..3308)
                     /gene="ydcU"
                     /locus_tag="ACHKAU_003855"
     CDS             complement(2367..3308)
                     /gene="ydcU"
                     /locus_tag="ACHKAU_003855"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5532833.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3309..4322)
                     /gene="ydcT"
                     /locus_tag="ACHKAU_003856"
     CDS             complement(3309..4322)
                     /gene="ydcT"
                     /locus_tag="ACHKAU_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5532834.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4340..5485)
                     /gene="ydcS"
                     /locus_tag="ACHKAU_003857"
     CDS             complement(4340..5485)
                     /gene="ydcS"
                     /locus_tag="ACHKAU_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO5532835.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5730..7135)
                     /gene="ydcR"
                     /locus_tag="ACHKAU_003858"
                     /pseudo
     CDS             complement(5730..7135)
                     /gene="ydcR"
                     /locus_tag="ACHKAU_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7214..7630)
                     /gene="hicB"
                     /locus_tag="ACHKAU_003859"
     CDS             complement(7214..7630)
                     /gene="hicB"
                     /locus_tag="ACHKAU_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO5532836.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7676..7852)
                     /gene="hicA"
                     /locus_tag="ACHKAU_003860"
     CDS             complement(7676..7852)
                     /gene="hicA"
                     /locus_tag="ACHKAU_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO5532837.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8074..8304
                     /gene="yncJ"
                     /locus_tag="ACHKAU_003861"
     CDS             8074..8304
                     /gene="yncJ"
                     /locus_tag="ACHKAU_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO5532838.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8396..10357)
                     /gene="rlhA"
                     /locus_tag="ACHKAU_003862"
     CDS             complement(8396..10357)
                     /gene="rlhA"
                     /locus_tag="ACHKAU_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO5532839.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10430..10966)
                     /gene="sutR"
                     /locus_tag="ACHKAU_003863"
     CDS             complement(10430..10966)
                     /gene="sutR"
                     /locus_tag="ACHKAU_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO5532840.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11019..12233
                     /gene="ydcO"
                     /locus_tag="ACHKAU_003864"
     CDS             11019..12233
                     /gene="ydcO"
                     /locus_tag="ACHKAU_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO5532841.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12273..>13113)
                     /locus_tag="ACHKAU_003865"
     CDS             complement(12273..>13113)
                     /locus_tag="ACHKAU_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5532842.1"
                     /translation="NSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTES
                     EVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFS
                     NNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVS
                     QPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTA
                     KENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQ
                     ATA"
BASE COUNT         3177 a         3522 c         3220 g         3194 t
ORIGIN      
        1 ggatgaaaaa tcgccatcaa acatttagcc taatttttac gctagttatt aatgtaatca
       61 ttctaatact taaccgccat taaactcaga acctaaaacg atagtggccg tcctataaca
      121 gccactaaca tctaacttaa tgtttaacca tgacgtggcg gacgacggtg taatcctcca
      181 gcccataaag tgacatatcc ttgccgtaac cagaaagttt ctgcccaccg tgcggcattt
      241 cacttaccag catgaaatgg gtattgaccc aggtacaacc atattgcagc cgtgcgctga
      301 cgcgatgcgc cctgcccaca tctttcgtcc ataccgaaga tgcaagtccg tactggctgt
      361 cattcgccca gttcaccacc tgttcttcgt tgtcgaaggg cgtaacactc actactggac
      421 caaatacctc tttttgcacg atggcatcgt cctgtaatgc gccagccagc agcgtcggcg
      481 catagtaata gccattaccc ttgcgctttt caccgccagt gatcactttg atgtgccctg
      541 tcgctttcgc ctcttctact gccttgctga cgcgttcgag atgcgccagc gagcttaaag
      601 gtccaagctc cgtagactcg tcatccggcg caccagattt taacgttgcc accgcagcac
      661 ccagtttttc caccagcgta tcgtaaatgc ctttttgcgc gtagatccga caagccgcag
      721 tacaatcctg tccagcattg taatagccaa atgtacgtac accttcgacc actgcttcaa
      781 tatccgcatc atcaaaaaca atcactggcg ctttgccacc aagttccata tgagtacgct
      841 taatggacga cgcggtatgg ctgatgatgt gctcgccggt ggcgatagag cccgtcagcg
      901 acaccatccg cactttggga tgaccggtca gcggatcacc caccgttttg cctctgccaa
      961 acagtacgtt aatcacgcct gccgggaaga tatctttcgc cagctctgcc aacttcaacg
     1021 cggtcagcgg ggtaatttct gatggtttaa gcactacgca gttccctgcc gccagcgccg
     1081 gagccagttt ccacgctgcc atcatcagcg gataattcca cggtgcgata gaagccacga
     1141 cccccaacgg atcgcgacgg atcatcgaag tatgaccttc aagatattca cctgccgcca
     1201 gaccattcag acagcgcgcc gcacccgcga aaaagcgaaa aacatcgaca atcgccggga
     1261 tttcatcatt gaacgcacta tgcagcggtt tgccacaatt acgggactcc agttcggcaa
     1321 aaacctgacc attttcttcg ataacatcag ccagtttcag cagacattcc gcacgcactt
     1381 tcggcgtggt ttgcccccat tcggcaaatg ctgcatctgc cgcgcgcaca gcagcatcga
     1441 cctgctctgc ggatgcctcg gcaatctcca gtaaaacgtc ccccgttgcc ggattataga
     1501 caggctgttt ttccccttcg ccgctaacca gttctccgtt aatcagtaac ttatgttgca
     1561 tagcatattc ctgtatcagt ggttattgac cattgtcgcc ttcgcgggtt agccaccagg
     1621 cccccaggat cggcaaggtt gttaccaaca taaccagcag tgccaccacg ttagttaccg
     1681 gtacatcacg cggtcgccca agctgattga gcaaccacaa cggtaacgtt cgttcatgac
     1741 ctgccgtaaa agtcgtaacg atgatttcat cgaacgacaa ggcaaacgcc agcattcctc
     1801 ctgccagtag cgccgaactg agattcggca acactacgta gcggaaggtt tgccagccat
     1861 tggccccaag atccattgac gcctcaacca gactccacga ggtgcggcga aaacgggcga
     1921 tgacattgtt aaacaccacc actacgcaaa aagtcgcatg accgaccacg atggtgaaaa
     1981 atcccggctc cagattgatg gttttaaaag cggttaatag cgccagacca gtgacaatgc
     2041 ccggcagcgc aatgggcagc agcagtaaca gcgaaatggc gtttttgccg aaaaagtctc
     2101 gtcgccacag cgcggcagct gccagcgtcc ctaacaccag cgccattaat gtcgccagcg
     2161 ccgccacttt aagtgacagt gtcacggcat caagaatatc actacgctgt gctgccacgc
     2221 taaaccagcg cagcgtcagg ccctgcggtg gaaaactaaa cgccgcatct tcagtgttaa
     2281 acgcataagc ggcgataatc aggatgggaa aatgtaggaa aaccacgccg ccccaggccg
     2341 ccagtttgag gaaaaacggt gcgcgttcag agtgcatcga acgctcccag acgtttcacg
     2401 aacgccaggt acagtgcgat gagaacaatc ggcaccaggg tgaatgccgc cgccatcggc
     2461 atattgccaa tcgccccctg ctgggaataa accatattgc cgataaaata tcctggagga
     2521 ccaaccagct gcgggacgat aaaatcgccc agtgtgagtg agaaggtaaa gatagagcca
     2581 gcggcaatac ccgggattgc cagcggcagc accacatagc gaaaggtttg tcgtggacgt
     2641 gcgccgagat cagccgacgc ctgcaacaat gacggcggca aacgctcaag cgccgcctga
     2701 acgggcagga tcatgaacgg caaccagata tagagaaaca ccagaaagcg ccccagcccg
     2761 gaagttgaca gcgtatttcc gcccaccgca ggtaatgtaa ggaacgcagt cagcagtggt
     2821 tccagcccaa gatgttgtaa aaaccactga gccacgccat cttttgccag caataacgtc
     2881 caggcatagg ctttaacaat gtagctcgcc cacatcggca acattaccgc aatataaaaa
     2941 aaagctttca ttttcccgct ggtatagcgc gccatatacc acgccattgg aaaagccaga
     3001 atggcgctgg cgatagtgac tgccacagcc atagtcagcg tgcggagaat gatgtcgtaa
     3061 ttcgccggat taaacagcgc acggatattc gccagcgtca gttccggcgt taccgacatg
     3121 gtgaaatcgt caaaagtata aaatccctgc cataacagtg tcagcagcga gccgaaatag
     3181 acaatgccaa accacattag cgggccaagc agcagtaaaa acagccccag cctcggatta
     3241 cgccagaaga aaccggacac cttacccaga cctggacgtg aaggtgattg caatacattc
     3301 atcgccattc acctctcctc aaccagcggc accatcacat cacgcgacca ggaaaccatc
     3361 acctgttgtc cgggcgtgag cgtggcaggc agttcttcgc ctgtcatatt ggcctgactc
     3421 acaagcagtt tttcaccgcc gttcaatttc agttcaaaac gagttgccgc gccctgatat
     3481 tgcaccgcct ggatcgtgcc attggcctgc agttcaccag gggtgttgag gcggatatgt
     3541 tccggtcgca gggcgaagct tcccgtcatg ccacaaagtt tctctgccat cagtccatca
     3601 aaaacgttgg atgtcccgac gaatccggca acaaacggcg tgcgcgggcg catatagaga
     3661 tcgcgcgggg aatcgacctg ctcaatgcgt ccattattga aaaccgccac acgatcggac
     3721 atcgataacg cttcgccctg atcgtgggta acgaagataa aagtgatacc gagagactgt
     3781 tgcagttttt tcagttccag ctgcatctgc tcacgcaatt tgagatccag tgcgccgagc
     3841 ggttcatcca acagcaatac gcgcggttca ttcaccaatg ctctggcgat agcaacccgc
     3901 tggcgctgac caccagaaag ttgtgacggt ttacgttgat gtacaaaccc caacgccact
     3961 ttctccagcg cctcttgcgc cattgcgtgc cgctgctttt tattcacgcc tttgaccatc
     4021 agcccatagg cgacattgtc aagaatcgac atatgcggaa atagcgcgta gtcctgaaag
     4081 acagtattca cgtcccgctc ccacggtggc agattgctgg cgggtttacc aaagatagag
     4141 atagccccgc cggaaagctg ttcgaagcca gcaatcaggc gcaggcaggt ggttttgccg
     4201 gagccggacg gccccagcat agagaagaac tcaccatctt ttatcgcaat actgacgcca
     4261 tctactgcgc gcacgtcacc gtacaaccgc gagacgttgt caaactccac tgcgtacgtc
     4321 ataaagcacc ccagcgaagt tagcgaccgc ccataatggc aatgtaatcc tgcgtccagc
     4381 gactgtaggg aacaaacttg cccccttctg ctataggcgt tttccagaag gcgattttgt
     4441 cgaaatagtt aaaaccgttg gtttcacaac ctttttcgcc taataacgga ctggctttac
     4501 acccttccgg cactaccggt aacgagccaa accaggccgc cacatcgccc tgcacttttg
     4561 gcgttaatga ccagttcatc catttgtagg cgcaaaccgg atgtttcgct tcgctatgca
     4621 gcatggtggt atcagcccaa ccggtaacac cctccttcgg gaaaacggta gcaacaggct
     4681 ggccttcggc tttcagggcg ttggcctgat agggccaggc actggaagca accacaccct
     4741 cgtttttgaa atcgctcatt tgcacggtag tgtcatgcca gtagcgatgg atcaaactgt
     4801 gttgagcgcg cagcactttc agcaccgcct ggtactgttc ttcggttagt tgatacggat
     4861 cgctgatgcc caactgcggc tgagtggctt taacgaacaa cgcagcgtcc gcaatgtaga
     4921 tagggccatc ataagcctga acgcggcctt tattgctctt gccgtccggc agattttgct
     4981 caacaaaaac cacttgccag ctatccggcg gcgtcgggaa ggttttagtg ttgtacatca
     5041 gcaggttcgg cccccattgg taaggtgtgc cgtaaacttt gccgccaaca ttaaaccagt
     5101 cgcctttaac cacgcgcgga tcgagcgttt tccagttggg aatcaaggcg gtattaatcg
     5161 gctgcacgcg tttgcccata atcaaacgca gcgaggcatc gccggatgcc gtaaccagat
     5221 cgtaaccccc tttggtcatc agactgacca tttcatcgga agtcgcggcg gttttcacat
     5281 tcaccgcgca gcctgtctct ttttcaaatt gcgttaccca gtcgtattgt ttatcagttt
     5341 gtccgcgttc gatgtatccc ggccaggcga taatatccag tcgcccttcc ggtttatcta
     5401 aattggtagg tggttcggcg gcgtgagcgg tcattattgt catggtgagc gcacacaggc
     5461 tgctgcgggc aaatgtcttg ctcataaggt cttactcctg tcgtaattaa ttgttcagcc
     5521 gccgtgccgt aaaaatatct ccttgtttaa ccatagacgg cggcttgcag gcacaccttc
     5581 gcccctgaga aaatttaatc aggttgtgag ctaccgcgca ttatgccgtc tgtaaggtta
     5641 tcttctggag tatagacaag gggttaggaa atatgactgc tattcgaaat tcatattacc
     5701 caatctaaga ggaattaaat taaacgctat tacagccgtt cttgaataag tttgcctaat
     5761 tgttttaccg cctgttcttc acgttctccc cactgccacg cggtattaaa acgaaaaaaa
     5821 cgtgaccagt tttcaccggt agaaaacatt ttacccggcg caatactgat atgatgcgtc
     5881 agtgccgcca ggcttaattc gccggcatct aacggctcgg ggagctccag ccagagaaaa
     5941 taaccgctgt cactatgatg aattttaact tccgcaggca gataacgcag cagtgcctgc
     6001 caggcacgtt gtttacgttc cgcaagctgg cgacgcaggc gacgaagatg ggcgtcgtat
     6061 cggcgcgtgg aaaggtaatc caccagcgca agctgcatcg gtgagctggt ggaaagcgta
     6121 ctcatcaact gcaagcgttg aattttacgt gcatgttttc cggcggcgac ccaaccaata
     6181 cgaaaaccag gcaccagaca tttcgaaaac gaagagcaat gcaaaacgcc atcgtggcga
     6241 tcccacgctt tcgcaggcag cggtttttcc cgtccaaaat aaagttcgct gtaaacgtca
     6301 tcttcaatca gcgttacgtt gtactgattg agtaacgcca ccagttgtgc tttttttgcg
     6361 gcgttaaggt aaatccgagt ggattctggc tattagtcat cagccagcac gctttcaccg
     6421 gatactcctg caacgccagt tccaacgcct gaagatctat cccttcttta acatccgtcg
     6481 ccaccgataa cgctttcagc cgtagccgtt ccagcgcctg caacgcgcca tagaaacaag
     6541 gattctctac tatcacccaa tcgcccggtt cagttaccgc ttgcaaactg aggtttaatg
     6601 cctctaacgc cccggcagta atgacaattt catcaggaga aatggtgatg ccctgtaagg
     6661 catagcgacg agcaatagcc tgacgcagtt ctgcgtttcc tggcggtaag ttttcaatca
     6721 cgctcatcgc cgtggcggtt ttgcttacct gcgccagcga gcggtttagt tgttggaggg
     6781 ggaaaagtcg cgggtcggga aaggccgagg caaacggaac gaccgacgga tcgcgactgg
     6841 cctgcaacat atcaaaaata taagtgttga tatcgactgc ttcatctcga gtgactggaa
     6901 tgactggcgc tttcggcatt tttattgcct gtggcgcaac gtaataaccc gactgcggtc
     6961 gtgcgataat atatccctga ctttcgagca actgataggc atggctgaca gtcataaagc
     7021 tcatgcctga aagcgccacc tggtcacgca acgaaggcaa acgatcgccg ggttgccaga
     7081 tacccgacgc aatctgctcg cgtaattgtt ctgcaagctg ctggtatttt ttcattatcg
     7141 aatcgtaatt atgtgcagat gattcggcag tctatatcag ttgttaaaaa tgacaacttt
     7201 tcgttaactg taattaaacc atcaccagcg ataactcttt gccaagcgcc tttgccgcaa
     7261 gctggacggc gtcgattttt gtcgcatggt gcaagttaaa taggcgagta atctcctgtt
     7321 taggtttgcc aattcgcctg gctaactctt gctgagtgat ttctgactgt aaaaaagcat
     7381 ttaacagcaa tactttagag gcgacgctca aaggtacttc aataaagtga tcatgactat
     7441 ttaatggcga aggtaacggg ataagctcgt tatcttcaaa ataaaaatca aatgcggtca
     7501 gtaaagcatc ttttgccgct tccatcgctt cagcgacagt ttcgccctgc gtcaacgctt
     7561 cagggatatc cacaaaagaa accatataac cgccttccgg cgcgggtgta agagtgacgg
     7621 gataacgcat aatttcgact gtccctcgca tgtcattaac ctttttaatt ggcgattaac
     7681 tcaaaccgag ttgtttcagg attgctttac gcaatggttc tttaatctca tcgcaggggt
     7741 gacgcggcat gacactgcgc ctcccatgaa acctgagttt caaatggttg ctgccattcg
     7801 ctacatcgac gccctgagat tcgagccaac gtctgaactc gctttgtttc acatcgcctc
     7861 cctgccgctg agtatatcaa caaagtaaac attagtgttt acctccgtgt gtaatttgct
     7921 cgaaaatagc actaaatcgc cgcaacggca gaatgccatg caaaatcagg aagaaagact
     7981 ccagaatttc tttgcgctaa tttgcaaaac tgaataataa cctcaagaaa cgatcggcat
     8041 catagcggca aaaggtaaga aaaaggtggc aggatgttta cgaaggcgtt atcggttgtc
     8101 ttattaacgt gtgcgctgtt ctcaggacaa ctcatggcag ggcacaaagg acatgaattt
     8161 gtgtgggtaa agaatgtgga tcatcagctg cgtcatgaag cggacagcga tgaattgcgt
     8221 gctgtggcgg aagagtcggc ggaaggtttg cgcgagcatt tttactggca aaaatcgcgc
     8281 aaaccagaag cgggacaacg ttgagtcatt tgttgccgga tgcggcgaga cgccttaccc
     8341 ggcaggcaat atcatgcaga ttcaatgagt tgtgagtaac cagaaaactg gcgttttacc
     8401 cttttcgctt aggcaatgtt ttcagcagct catccggact tacggatgcc actacgcttc
     8461 ccggtaacgg cattttcagt atgtgatttt tgattttgcc aatgacgtgc atctcgcatg
     8521 ggcggcaatc aaactttagc gttaatactt catcgccgtt aaccagttgc attggcgtcg
     8581 ccttccagct tttgatattg cctttcgcct gtttcgggca cagattaaag gcaaagcgca
     8641 gacaatgctt ggtgatcatc accgggactt cgcccttctc ttcatgtgct tcatacgccg
     8701 cgtcaatcag ctgcacacca tagcgatgat aaaattcacg cgctttctgg ttgtatacgt
     8761 tcgcgaagaa actcagatgc gtttgcggat aaaccggcgc aggatcagca accggtttac
     8821 ggctgccgcg ctggtaactg gcaagacgcg cagcatccag catgtcagca gcttcacggc
     8881 ggaactggtt taacagactg tttggtacaa acagcgcccc cggcaaatta atttgcacat
     8941 cgcgggcgta atagagggtt tgccccagtt ttgccagacc atccttcaga ttgttcattg
     9001 ctttttcggc gttattggct tcgtcgaact gcccgtccag cgtatgcgtg atgctgacac
     9061 cctcttcact ggtgagggtc agaatcagtt gttcctgcca gccgcccagt tcaatgtcta
     9121 ccgccacccg acgttcgctg gaggtttttg tcagtgcctg ctgccagtta tgatcaagat
     9181 tacggtttag tgggtgatgt ggacgaattt tgtgcaaatc tgctggcatt tcattgggcc
     9241 agacgcggta ctgattttct ccggttttct cgaccgtatt ggcacgaaaa ccgacgactt
     9301 cacgtttaat catcacgttc aggccatcac catttgccag tggctcggtg acggcaacat
     9361 cgagatgatc tttcgccact ttcaatactt cgcctaccgg caggccgata aatttcggcg
     9421 aatcgaacgc gccaatatcg cctttacggg cattcacaaa ataatctgtg ctaccacggt
     9481 ggaaagtctt ttccgtcgat ggaacaaaga aatgttcagt acgacctgat gaagcgcgcg
     9541 ccagatcgcc acgttcttca ataatggcat caagcatctg gcgataatgg gcggtgatat
     9601 tcttcacgta gctcatatct ttgtaacgcc cttcaatctt gaaggagcgt acgccagcat
     9661 caatcagcgc gccgaggttg gcagtctgat cgttatcttt catcgacagc agatgttttt
     9721 cataggaaac cacccgcccc tgatcgtctt tcaatgtgta tggcaaacgg cacgcctgcg
     9781 agcaatcacc acggttggcg ctacgccctg tttgcgcatg agaaatgtag cactgacccg
     9841 aataggccac gcacagtgcc ccatgaataa agaattcaat ggtcgcgtcc gtggcctggt
     9901 gaatcgcgcg gatctgctca agattcagct ctcgcgccag cacaatctgc gtgaagccaa
     9961 catcagagag gaacttcgct ttttctactg tacgaatgtc gcactgcgta ctggcgtgca
    10021 gttcaatcgg cggaatatca agttccagaa tccccatatc ctgaacaatc agcgcatcga
    10081 caccggtctg gtagaggtcg gtaatcagcc gttgcgcggg ttccagctca tcatcatgca
    10141 aaatggtgtt aagcgtgacg aaaatttttg caccataacg atgggcaaac ggcaccagct
    10201 cggcaatatc tttcaggcta ttactggcat tatgacgggc accaaaacca gggccgccga
    10261 tataaacagc atcggcaccg tgcaaaatag cttcgcgggc aatggcggca tcgcgtgccg
    10321 ggcttaacag ttcaagtcga tgagaagata cggtcacatt cttatccagt agctgaaaaa
    10381 tggcggctat tttagccaca agcgcgacgt tgcgaaatag ttttcccgct taactgcgcg
    10441 ggtaatggat gagggaatga aaatgtacgg tttgctcccc accattacga tagatatgcg
    10501 tgacgtctgc agcaaatcga acgccttccc cacaattaag ggtctgccat tccccatcaa
    10561 cgcacagatc aagttgtcca tcaatgacga cgacatgttc gattaccccc ttctcatggg
    10621 gcgtcgattc gcttattgca ccagatgcca tctgaatcga gaagtgttcg aagcagagtt
    10681 gtggatcata aggaaacagc gaagtgatca ccatcgcctg ctgttgtgga tcataaactg
    10741 aaggcgtagc ggactgcggc ggagaaataa atgttgaaaa tggtacgttc aacccggtgg
    10801 caattttcca taatgtcgcg accgtcggac tggactcatt acgctcaatc tgccctaaca
    10861 ttgctttaga aacgcccgtc gcttctgcca gtcgcgaaag actccagccg cgctgctggc
    10921 gtagttgttt aagtgtggtg gataaaaagc gagcgagatt ttccatgcga ctcctccggc
    10981 aaaacggaag tttatcactt gtgcgttata acggacaaat gctacggtgc ctgtacgcta
    11041 taacgcacga ggtgactatg cgtctgtttt ctattcctcc acccacgcta ctggcggggt
    11101 ttctggcggt attaattggc tacgccagtt cagcggcaat aatctggcaa gcagcgattg
    11161 tcgccggagc caccactgca caaatctctg gctggatgac ggcgctgggg ctggcaatgg
    11221 gcgtcagtac gctgactctg acattatggt atcgcgtacc tgttctcacc gcatggtcaa
    11281 cgcctggcgc ggctttgttg gtcaccggat tgcagggact aacacttaac gaagccatcg
    11341 gcgtttttat tgtcaccaac gcgttaatag tcctctgcgg cataacggga ctctttgctc
    11401 gtctgatgcg cattattccg cactcgcttg cggcggcaat gcttgccggg attttattac
    11461 gctttggttt acaggcgttt gccagtctgg acggtcaatt tacgttgtgt ggaagtatgt
    11521 tgctggtatg gctggcaacc aaggccgttg cgccgcgcta tgcggtaatt gccgcgatga
    11581 ttattgggat cgtgatcgtt atcgcgcaag gtgacgttgt cacaactgat gttgtcttta
    11641 aacccgttct ccccacttat attacccctg atttttcgtt tgctcacagc ctgagcgttg
    11701 cactccccct ttttctggtg acgatggcat cgcaaaacgc accgggtatc gcagcaatga
    11761 aagcagccgg gtattcggct cctgtttcgc cattaattgt atttactgga ttgctggcac
    11821 tggttttttc ccctttcggc gtttattccg tcggtattgc ggcaatcacc gcggctattt
    11881 gccaaagccc ggaagcgcat ccggataaag atcaacgttg gctggcagct gccgttgcag
    11941 gcatttttta tttgatcgca ggtctgtttg gtagtgctat taccgggatg atggctgccc
    12001 tgcccgtaag ttggatccag atgctggcag gtctggcgct gttaagtacc attagcggca
    12061 gtttgtatca ggcgctgcat aatgagcgtg agcgcgacgc ggcggtggtg gcatttctgg
    12121 taacggcaag tggattgacg ctggtcggga ttggttctgc gttctgggga ttaattgccg
    12181 gaggcgtttg ttacgtggtg ttgaatttaa tcgctgacag aaaccgatat tgacatcctc
    12241 cacgccctga aggacgagga tccctgctac gttcaggctg tcgcctgaat catttcggtg
    12301 ggttcctgct tcaacgggcg gcctgactgc accatctctc cacaggcaag aacggcgtgc
    12361 cccgccgcta aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc
    12421 tggcatctga atttactttg tgacaggcgg ttttctttcg ctgtatgacc acagcacgcg
    12481 caacgctggc ttgtgtacgc tggcggaaca gcaagcacct gaccgccacg ccagagctgc
    12541 ttgtactcaa gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa
    12601 cctgattttg cccggacatt gcgccccggc tggctgaccg tacccgctgc tgactttgac
    12661 atgttgctga ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt
    12721 gtgactttgt gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt
    12781 ttgcgtttct gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga
    12841 agtctcgcca gcgtcttctg gtttttctga aaactgttta caggctcaaa gactgtgcca
    12901 tctgacagcg tggcgagttt agccacgcca gcatccagcc cgaccattga tgctgaaggg
    12961 tgagccggag tggatacttc actttctgtc tgaatactga tgtaccactt accgcaggac
    13021 tggctgacag tgacattttt cacaacaccc gtgacctgac ggctattccg gtagcgcatc
    13081 cagcccagtt tcggcagaaa aatacggctg ttt
//
LOCUS       DAZFJF010000118        13024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFJF010000118 DAZFJF010000000
VERSION     DAZFJF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.118"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            225..560
                     /gene="yjdM"
                     /locus_tag="ACHKAU_003866"
     CDS             225..560
                     /gene="yjdM"
                     /locus_tag="ACHKAU_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO5532843.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            720..1163
                     /gene="yjdN"
                     /locus_tag="ACHKAU_003867"
     CDS             720..1163
                     /gene="yjdN"
                     /locus_tag="ACHKAU_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO5532844.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1296..2084
                     /gene="phnC"
                     /locus_tag="ACHKAU_003868"
     CDS             1296..2084
                     /gene="phnC"
                     /locus_tag="ACHKAU_003868"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO5532845.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2109..3125
                     /gene="phnD"
                     /locus_tag="ACHKAU_003869"
     CDS             2109..3125
                     /gene="phnD"
                     /locus_tag="ACHKAU_003869"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5532846.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLEKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3267..4046
                     /gene="phnE"
                     /locus_tag="ACHKAU_003870"
     CDS             3267..4046
                     /gene="phnE"
                     /locus_tag="ACHKAU_003870"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO5532847.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4067..4792
                     /gene="phnF"
                     /locus_tag="ACHKAU_003871"
     CDS             4067..4792
                     /gene="phnF"
                     /locus_tag="ACHKAU_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO5532848.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4793..5245
                     /gene="phnG"
                     /locus_tag="ACHKAU_003872"
     CDS             4793..5245
                     /gene="phnG"
                     /locus_tag="ACHKAU_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO5532849.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5242..5826
                     /gene="phnH"
                     /locus_tag="ACHKAU_003873"
     CDS             5242..5826
                     /gene="phnH"
                     /locus_tag="ACHKAU_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO5532850.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5826..6890
                     /gene="phnI"
                     /locus_tag="ACHKAU_003874"
     CDS             5826..6890
                     /gene="phnI"
                     /locus_tag="ACHKAU_003874"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO5532851.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6883..7728
                     /gene="phnJ"
                     /locus_tag="ACHKAU_003875"
     CDS             6883..7728
                     /gene="phnJ"
                     /locus_tag="ACHKAU_003875"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO5532852.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDTGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7725..8483
                     /gene="phnK"
                     /locus_tag="ACHKAU_003876"
     CDS             7725..8483
                     /gene="phnK"
                     /locus_tag="ACHKAU_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO5532853.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8594..9274
                     /gene="phnL"
                     /locus_tag="ACHKAU_003877"
     CDS             8594..9274
                     /gene="phnL"
                     /locus_tag="ACHKAU_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO5532854.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9271..10407
                     /gene="phnM"
                     /locus_tag="ACHKAU_003878"
     CDS             9271..10407
                     /gene="phnM"
                     /locus_tag="ACHKAU_003878"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO5532855.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10407..10964
                     /gene="phnN"
                     /locus_tag="ACHKAU_003879"
     CDS             10407..10964
                     /gene="phnN"
                     /locus_tag="ACHKAU_003879"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO5532856.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10951..11385
                     /gene="phnO"
                     /locus_tag="ACHKAU_003880"
     CDS             10951..11385
                     /gene="phnO"
                     /locus_tag="ACHKAU_003880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO5532857.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11387..12145
                     /gene="phnP"
                     /locus_tag="ACHKAU_003881"
     CDS             11387..12145
                     /gene="phnP"
                     /locus_tag="ACHKAU_003881"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO5532858.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12292..12621)
                     /gene="yjdP"
                     /locus_tag="ACHKAU_003882"
     CDS             complement(12292..12621)
                     /gene="yjdP"
                     /locus_tag="ACHKAU_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO5532859.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12690..13007)
                     /locus_tag="ACHKAU_003883"
     CDS             complement(12690..13007)
                     /locus_tag="ACHKAU_003883"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO5532860.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2857 a         3929 c         3663 g         2575 t
ORIGIN      
        1 gtgaattaaa aaaaagaaca agtagagcat taacattatc ttaaataata aatggaggca
       61 aaaagattat tttctgtttg cgtttccttt caaatgaaaa cgatcgtcgt ctaaaatcag
      121 cagtaccccc gacaaactca gggattttgt gtataattgc ggcctttttc ggcaatctgc
      181 cgttttttgg cgcttttgcc ctgctgactt ttgaggaaat ccacatgtca ttaccacact
      241 gcccaaaatg caactccgaa tacacttacg aagataacgg catgtacatc tgcccggaat
      301 gtgcctacga atggaacgac gcagaacctg cacaggaaag cgacgagctg atcgttaaag
      361 atgctaacgg caatctgctg gctgacggcg acagcgttac catcattaaa gatctgaagg
      421 tgaaaggtag ctcttcgatg ctgaaaattg gcaccaaagt gaaaaacatc cgcctggttg
      481 aaggcgacca taacatcgat tgcaaaatcg acggttttgg tccgatgaaa ctgaaatctg
      541 agtttgtgaa aaagaactga ttgtattgtg atcggtaagc cggataaggc gctcgcgccg
      601 catccggcaa cgatgccgac tgcctgatgc gacgctgacc gcgtcttatc aggcttccct
      661 ctcatctgta taaatttcga actacactta actggcttct cttaactgag gtcaccatca
      721 tgtcgttaag tccctacctc tcttttgccg gtaactgtgc cgacgcaatt gcttattatc
      781 aacgtacgtt gggcgctgaa ctgctctata aaatcagctt cggcgaaatg ccaaaatcag
      841 cgcaggacag cgccgagaac tgcccttccg gaatgcaatt tcccgatacc gccatcgccc
      901 acgccaacgt gcgcattgcc ggaagcgaca tcatgatgag cgatgctata ccgtcaggaa
      961 aagccagcta ctccggcttt acgctggtgc tcgattcgca acaggtcgaa gaaggaaaac
     1021 gctggtttga caatcttgcc gctaacggaa aaatcgaaat ggcctggcag gaaactttct
     1081 gggcgcatgg ctttggcaaa gtcaccgata aatttggcgt accgtggatg attaatgtcg
     1141 tcaaacaaca accaacgcaa taacccgccg ggaggcccgc cctcccgcac tgtcatcgaa
     1201 ttcccgttaa ctcttcatct gttagtcact tttaattaac caaatcgtca caataatccg
     1261 ccacgatgga gccacttttt tagggaggct gcatcatgca aacgattatc cgtgtcgaga
     1321 agctcgccaa aaccttcaat cagcatcagg cgctgcatgc ggttgatctg aacattcatc
     1381 acggtgaaat ggtggctctg cttgggccgt cgggttccgg caaatccacc cttttacgtc
     1441 acttaagcgg tttgattacc ggcgataaat ccgccggcag ccatatcgag ctgctgggcc
     1501 gcacagtcca gcgcgaaggc cgtctggcgc gcgatatccg caaaagccgc gccaacaccg
     1561 gctacatctt ccaacaattc aacctggtga accgcctgag cgtactggag aacgtgctga
     1621 ttggcgcgct cggcagcacg ccgttctggc gcacctgttt tagctggttc accggcgagc
     1681 agaaacagcg cgcgttacag gcgctgaccc gcgttggcat ggtgcatttt gcccatcagc
     1741 gcgtttccac cctctccggc ggacagcagc agcgtgtggc gattgcccgc gcgctgatgc
     1801 agcaggcgaa ggtgattctg gccgatgaac ccatcgcctc gctggacccg gaatccgccc
     1861 gcatcgtgat ggacaccctg cgcgacatca atcagaacga cggcatcacc gtggtcgtca
     1921 cgctgcatca ggtggattac gccctgcgct actgcgaacg catcgtcgcc ctgcgccagg
     1981 ggcacgtttt ctacgacggc agcagccaac agtttgataa cgaacgtttt gaccatctct
     2041 accgcagcat taatcgcatc gaagagaacg cgaaagctgc ctgacatccc catcattgag
     2101 gaaaacgaat gaacgctaag ataattgcct cgctggcctt caccagcatg ttcagcctca
     2161 gcaccctgtt aagcccggca cacgccgaag agcaggaaaa ggcgctgaat ttcggcatta
     2221 tttcaacgga atcacagcaa aacctgaaac cgcaatggac gccattctta caggatatgg
     2281 agaagaagct gggcgtgaag gtgaacgcct tctttgcccc agactacgca ggcattatcc
     2341 agggaatgcg cttcaataaa gtggatatcg cctggtacgg caacctgtcg gcaatggaag
     2401 cggtggatcg cgccaacggc caggtcttcg cccagacggt cgcggcggat ggatcgccag
     2461 gttactggag cgtgttgatc gtcaacaaag atagtccgat caacaacctg aacgatctgc
     2521 tggcgaagcg gaaagatctc accttcggca atggcgatcc taactccacc tctggcttcc
     2581 tcgtccccgg ttactacgtc tttgccaaaa acaatatctc cgccagcgac tttaagcgca
     2641 ccgtcaacgc tgggcatgaa accaacgcgc tggccgtcgc caacaagcag gtggatgttg
     2701 ccaccaacaa caccgaaaac ctcgacaagc tgaaaacctc cgcgccagag aagctgaaag
     2761 aactgaaggt gatctggaag tcgccgctga tcccaggcga tccgatcgtc tggcgtaaaa
     2821 acctttccga aaccaccaaa gacaagatct acgacttctt tatgaactac ggcaaaacgc
     2881 cggaagaaaa agcggtgctg gaacgcctgg gctgggcgcc gttccgcgct tccagcgacc
     2941 tgcaactggt gccgattcgc cagctcgcgc tgtttaaaga gatgcagggc gtgaaaagca
     3001 ataaaggact gaatgagcag gacaagctgg aaaaaaccac cgagattcag gcgcagctgg
     3061 atgacctgga ccgcctgaac aacgcgttaa gcgcgatgag ttcggtgagt aaagcggtgc
     3121 agtaaatcgt aggtcggata agacgccccg gcgtcgcatc cgacaatgtg caggcgttga
     3181 tgccggatgc ggtgcaagca ccttatccgg cctacagacc ggataaggtg cttgcaccgc
     3241 atccggccta cagaccggag ccaaacatgc aaaccatcac catcgcccca cccaagcgca
     3301 gctggttctc gcttctgagc tgggccgttg ttctcgccgt gttggtcgtc tcgtggcagg
     3361 gcgcggaaat ggccccgctc acgctgatta aagacggcgg caacatggcg acgttcgccg
     3421 ccgacttctt cccgcccgat ttcagccagt ggcaggatta cctcaccgaa atggccgtca
     3481 cgctgcaaat cgccgtctgg ggcacggcgc tggcggtggt tctctccatc cccttcggcc
     3541 tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca gcccgttcgc cgcctgatgg
     3601 acgcctgccg cgtcattaac gaaatggtct tcgccatgct gttcgtggtc gccgtcggcc
     3661 tcggcccgtt cgctggcgtg ctggcgctgt ttatccacac caccggcgtg ctctccaaac
     3721 tgctttccga agcggtggaa gcgattgaac ccggcccggt ggaaggcatt cgcgccaccg
     3781 gcgccaacaa gctcgaagag atcctctacg gcgtgctgcc gcaggtgatg ccgctgctga
     3841 tctcctactc cctctatcgc ttcgaatcca acgtccgctc ggcgaccgtc gtcggcatgg
     3901 tcggtgcagg cgggatcggc gtcaccctgt gggaagcgat tcgcggtttc cagttccaac
     3961 aaacctgcgc cctgatggtg cttatcatcg tcacggtcag cctgctggat ttcctctctc
     4021 aacggttgcg taagcacttt atctgataag cgaggcattg atatctatgc acttgtctac
     4081 acatccgacc agctacccaa cacgctatca ggagatagcc gcaaaacttg agcaggagct
     4141 tcgtcaacac taccgctgcg gcgactatct tcccgccgag cagcaactgg cggcgcgctt
     4201 tgaggtgaac cgccacaccc tgcgccgcgc catcgaccaa ctggtggaaa aaggctgggt
     4261 acagcgccgt cagggcgtcg gcgtgctggt gctgatgcgc ccgttcgatt acccgctcaa
     4321 cgcccaggcg cgttttagcc agaacctgct ggaccagggt agccatccca ccagcgaaaa
     4381 actgctgtcg gtattgcgcc ccgcgtccgg tcacgtcgct gacgcgctgg ggattaccga
     4441 gggcgagaac gttatccatt tgcgcaccct gcgtcgcgtc aacggcgtcg cgctctgttt
     4501 aatcgaccac tactttgcgg acctcaccct ttggccgacg ctgcaacgct tcgacagcgg
     4561 ttcgctgcac gattttctgc gcgagcaaac cggaattgcg ctgcgccgca gccagacgcg
     4621 gatcagcgcc cgtcgcgccc aggccaaaga gtgccagcgt cttgaaattc cgaatatgtc
     4681 gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt gaaagcagcc cggcggagta
     4741 ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact atggagcact gaatgcacgc
     4801 agataccgcg acccgccagc actggatgtc cgtactggcg cacagccaac cggctgaact
     4861 ggcagcccgc ctgaaagcac tgaacatcac cgccgactat gaggttatcc gcgccgccga
     4921 aaccggcctg gtgcagattc aggcgcggat gggcggcacc ggcgaacgct tttttgccgg
     4981 cgacgccacg ctcacccgcg ccgccgtacg cctgactgac ggcacgctcg gctacagctg
     5041 ggtgctgggg cgcgacaaac agcacgccga acgctgcgcg ctgattgacg cgctgatgca
     5101 gcaatctcgc tattttcaaa acttatcaga aacccttatt gccccgctgg acgctgaccg
     5161 tatggcacgc attgccgcac gccaggccga agtgaacgcc agccgggtcg acttctttac
     5221 gatggttcgc ggagacaacg catgaccctg gaaaccgctt ttatgcttcc cgtgcaggat
     5281 gcccagcaca gttttcgtcg cctgttaaag gccatgagcg agccgggcgt gattgtcgcc
     5341 ctgcatcagc tcaagcgcgg ctggcagccg ctgaatatcg ccaccaccag cgtgctgctg
     5401 acgctggccg ataacgacac gccggtgtgg cttgccgcgc cattgagtaa cgatatcgtc
     5461 agccagagcc tgcgttttca taccaacgcg ccgctggtca gccagccgga acaggcgacc
     5521 ttcgcggtga cggatgaggc gatttccagc gaacagctta acgccctttc caccggcacc
     5581 gccgttgcgc cggaagcggg tgcaacgctg attttacagg tcgccagcct gagcggcggg
     5641 cgcatgttac gtctcaccgg cgcgggcatt gccgaagaac gaatgatcgc tccgcagctg
     5701 ccggagtgca ttctgcacga actcaccgag cgcccgcacc cgttcccgct cggcatcgac
     5761 ctgatcctga cctgcggcga gcgcctgctg gctattccgc gaaccactca tgtggaggtg
     5821 tgctgatgta cgttgccgtg aaagggggcg agaaggcgat cgacgccgct cacgccctgc
     5881 aagagagccg acgccggggc gataccgatt tgcctgaact gagcgtcgcc cagattgaac
     5941 agcagcttaa tcttgcggtg gatcgcgtga tgaccgaagg cggcattgcc gaccgcgaac
     6001 tggcggcgct ggcgctgaaa caggccagcg gcgataacgt cgaagcaatt ttcctgctgc
     6061 gcgcctaccg caccacgctg gcgaagctcg cggtaagcga gccgctcgac accacagaga
     6121 tgcgtctcga acggcgtatt tcggcggttt ataaagacat tcccggcggc cagctgcttg
     6181 gcccaaccta cgactacacc catcgcctgc tcgatttcac cctgctggca aacggtgaag
     6241 cgccgacgct gaccaccgcc gacagcgaac aacagccgtc gccgcacgtt ttcagcctgc
     6301 tggcgcgtca ggggctggtg aagtttgaag aagatagcgg cgcacagccg gatgacatca
     6361 cccgcacgcc gccggtttac ccctgctcac gctcctcccg cctgcaacaa ttaatgcgcg
     6421 gggacgaagg ctatttgctg gcgctggcct actccaccca gcgtggttac gggcgcaatc
     6481 acccgttcgc gggcgagatc cgcagtggtt acatcgacgt gtcgattgtg ccggaagagc
     6541 tgggatttgc ggtaaacgtc ggcgaactac tgatgaccga gtgtgaaatg gtcaacggtt
     6601 ttatcgaccc gccggatgag ccgccgcact tcacgcgcgg ctacgggctg gtattcggca
     6661 tgagcgagcg caaagcgatg gcaatggcgc tggtcgatcg tgcgttgcag gctccggaat
     6721 acggcgagca cgcgacaggc ccggcgcagg atgaagagtt tgtgctggca catgccgaca
     6781 acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc ccactacgtc gatttccagg
     6841 ccgaactgga gctactcaaa cgtctgcaac aggagaagaa ccatggctaa tctgagcggc
     6901 tacaactttg cctacctcga cgagcagacc aaacgcatga tccgccgcgc tatcttaaaa
     6961 gcggtggcga tccccggtta tcaggtgccg tttggcgggc gcgagatgcc gatgccatac
     7021 ggctggggaa ccggcggcat acagctcacc gccagcgtga ttggcgaaag cgacgtgctg
     7081 aaggtgattg accagggtgc ggatgacacc accaacgccg tgtcgattcg caacttcttt
     7141 aagcgcgtga ccggggtaaa caccactgaa cgtacggacg atgcgacgct tatccagacg
     7201 cgtcaccgca tccccgaaac gccgctgacc gaagatcaga tcattatctt ccaggtgcca
     7261 atcccggaac cgctgcgctt tatcgagccg cgcgaaacgg aaacccgcac catgcacgcg
     7321 ctggaagagt acggcgtgat gcaggtgaaa ctgtatgaag atatcgcccg cttcggtcat
     7381 atcgccacta cctatgccta tccggtgaag gtgaacgggc gctacgtaat ggacccgtcg
     7441 ccgatcccga aattcgataa cccaaaaatg gacatgatgc ccgccctgca actgttcggc
     7501 gcggggcgcg agaagcgcat ctatgcggtg ccgccgttta cccgcgtgga aagtctcgat
     7561 ttcgacgatc acccgttcac cgttcagcag tgggatgagc catgcgccat ctgcggatcg
     7621 acccacagct atcttgatga agtggtgctg gatgacaccg gaaaccgcat gtttgtctgc
     7681 tccgataccg attattgccg ccaacagagc gaggcaaaaa accaatgaat caaccgttac
     7741 tttcggtcaa taacctgacc cacctttacg cgccgggcaa aggctttagc gatgtctctt
     7801 ttgatttatg gccgggggaa gtgctgggca ttgtcgggga gtccggctcc gggaagacca
     7861 cgctgctgaa atcaatctcc gcgcgcctga cgccgcagca gggggagatt cactatgaga
     7921 gccgttcgct gtacgcaatg agcgaggccg accgccgtcg cctgctgcgc accgaatggg
     7981 gcgtagtgca tcagcatcca ctcgacggcc tgcgccgtca ggtctcggca ggcggcaata
     8041 tcggcgagcg gctgatggcg accggggcac gtcattacgg cgatattcgt gccaccgcgc
     8101 agaagtggct ggaagaggtg gaaattcccg ccaaccggat cgacgacctg ccgaccacct
     8161 tttccggcgg catgcagcaa cgtttgcaga tcgcccgcaa cctggtgacg catccgaagc
     8221 tggtgtttat ggacgagccg accggcggac tggatgtgtc ggtacaggca cgcctgctcg
     8281 acctgctgcg cggcctggtg gtggagctga acctcgcggt agtgattgtc acccacgatt
     8341 taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat gaagcagggg caagtggtgg
     8401 agagtgggtt aaccgaccgc gtgctcgacg acccgcacca tccgtataca caactgctgg
     8461 tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg cgtaaacgcc ttatccggcc
     8521 tacaaatgcg ctccccgtag gtcggataag acgcgtcagc gtcgcatccg acacccgaac
     8581 cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa accttcatcc tgcaccagca
     8641 aaacggcgtg cgcctgcccg tcttacaacg tgcctcgctc accgtcaacg cgggcgaatg
     8701 cgtggtgctc cacggccatt ccggcagcgg caaatcaacc ttgctgcgct cgctgtacgc
     8761 caactatctg cccgacgaag ggcaaatcca gatcaaacac ggtgacgaat gggtcgatct
     8821 ggtcaccgcg ccagcgcgca aagtggtgga gatccgcaaa accaccgtcg gctgggtgag
     8881 ccagtttctg cgcgtcatcc cgcgtatttc agcactggaa gtggtgatgc agccgctgct
     8941 cgataccggc gttccgcgtg aagcctgcgc cgctaaagcc gcgcgtctgc tcacccgcct
     9001 gaacgtgccg gaacgcctgt ggcacctggc gccatcaacc ttttccggtg gcgaacagca
     9061 gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc attctgctgc tcgacgaacc
     9121 taccgcctcg ctggacgcca aaaacagcgc cgccgtagtc gagctgattc gcgaagccaa
     9181 aacccgtggc gcggccatcg tcggcatctt ccatgacgaa gccgtacgta atgacgtcgc
     9241 cgaccgcttg cacccaatgg gagcctctgc atgattatca ataacgtcaa actggtgctg
     9301 gaaaacgaag tggtacacgg ttcgctggag gtgcaggatg gcgaaatccg cgcctttgcc
     9361 gaaagccaga gccgcctgcc agaagcgatg gacggcgaag gtggctggct actacccggc
     9421 ctgattgagc tgcataccga caacctcgat aaattcttca ccccgcgccc gaaagtcgac
     9481 tggcccgccc attcggcgat gagcagccac gacgcgctga tggtggcaag cggcatcacc
     9541 accgtactgg acgccgtggc gattggcgac gtgcgcgacg gcggcgatcg gctggagaat
     9601 ctggagaaga tgatcaacgc catcgaagag acgcaaaaac gcggcgtcaa ccgcgccgag
     9661 caccgcctgc acctgcgctg cgaactgccg catcacacca cactgccgct gtttgaaaaa
     9721 ctggttcagc gcgaaccggt gacgctggtg tcgctgatgg accactcacc gggccagcgc
     9781 cagttcgcta accgcgagaa gtatcgcgaa tattatcagg gcaaatactc cctcaccgat
     9841 gcgcagatgc agcagtacga agaagagcaa ttggcgctcg ccgcacgctg gtcgcagccg
     9901 aatcgcgaat ccatcgccgc cctgtgccac gcgcgacaaa ttgcgctcgc cagccacgat
     9961 gacgccacgc acgcccacgt tgccgaatcc caccaacttg gcagcgtgat cgccgaattt
    10021 cccaccacgt tcgaagcggc ggaagcctcg cgtaagcatg gcatgaacgt gctgatgggt
    10081 gcgccaaata tcgtgcgtgg cggctcgcac tccggcaatg tggcggccag tgaactggcg
    10141 cagcttggtc tgctggatat cctctcttcc gactactacc ccgccagcct gctggatgcg
    10201 gcgttccgcg tcgccgatga cgagagcaac cgctttacgc taccgcaggc ggtgaggctg
    10261 gtgaccaaaa atccggcgca ggcgctgaat cttcaggatc gcggggtgat tggcgagggt
    10321 aaacgcgctg acctggtgct ggcgcatcgc cagggcaatc acattcatat cgaccacgtc
    10381 tggcgtcagg gtaaaagggt gttctgatga tgggaaaact gatttggtta atggggccgt
    10441 ccggctccgg gaaagacagc ctgctggcgg aactccgcct gcgggaacaa acgcagttac
    10501 tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg aagtgaaaac catatcgccc
    10561 tgagtgagcg ggagtttttt acccgcgcgg ggcaaaacct gctggcctta agctggcacg
    10621 ctaacggtct gtattatggc gtcggcatcg agattgacct ctggctgcac gctggattcg
    10681 acgtagtcgt caacggttca cgcgcgcatt tgccgcaggc acgggcgcgc tatcaatcgg
    10741 cgctgctgcc cgtctgttta caggtttcgc cggagatcct gcgccagcgc ctggaaaacc
    10801 gtggccgtga aaacgccagt gaaattaacg cccggctggc gcgcgccgcc cgctatactc
    10861 cacaggattg ccatacgctc aacaatgacg gcagcctgcg ccagtcggtc gacacactgc
    10921 tgacgctgat tcatcagaag gagaaacacc atgcctgctt gtgagcttcg cccggccacg
    10981 cagtacgata ccgacgcggt ttacgcgctg atttgtgagc taaaacaggc ggagtttgac
    11041 caccacgcgt ttcgcgtggg ctttaacgcc aacctgcgcg acccgaacat gcgctaccat
    11101 ctggcgctgc tggatggcga agttgtcggc atgatcggcc tgcatttgca gtttcatctg
    11161 catcatgtca actggatcgg cgaaattcag gaactggtgg tgatgccgca ggcgcgcggc
    11221 ctgaacgtcg gtagcaagtt gctggcgtgg gcagaagaag aagcccgcca ggccggggcc
    11281 gaaatgaccg agctttcgac caacgtgaag cgccacgacg cgcaccgttt ctatctgcga
    11341 gaaggctacg agcagagcca cttccgcttc accaaggcgc tgtaacatga gcctgaccct
    11401 cacgctcacc ggcactggcg gcgcacaggg cgttccggca tggggctgcg agtgcgcggc
    11461 ctgcgccaga gcgcggcgct cgccgcagta tcgccgccag ccatgcagcg gcgtagtgaa
    11521 gtttaacgac gcgatcaccc tgatcgacgc cgggcggcac gatctcaccg atcgctggtc
    11581 gcctggatcg ttccagcagt ttttgctgac gcactaccat atggatcacg tccaggggct
    11641 atttccgcta cgctggggcg tgggcgatgt gatcccggtc tacggcccgc cggatgaaca
    11701 gggctgcgac gatctgttta aacatccggg cctgcttgat ttcagccaca cagtggaacc
    11761 gtttgtggtg tttgatttgc aggggttgca ggtcacgccg ctgccgctta accactcaaa
    11821 actgaccttc ggttatctgc tggaaacggc acatagccgg gtggcgtggc tgtctgacac
    11881 cgctggcctg ccggaaaaaa cgctgaaatt tttactcaat aatcatccgc aggtgatggt
    11941 gattgattgc agtcacccgc cgcgcgcgga tgcaccgcgt aatcactgtg atttaaatac
    12001 cgtgcttgcg ctgaatcagg ttatccgctc gccacaggtg attctgaccc atatcagcca
    12061 ccagtttgat gcgtggctga tggaaaacgc actaccgtca gggtttgagg tggggtttga
    12121 tgggatggag attggggtgg cgtgatgaga gggaatgtgc gcgctggccc cctcacccta
    12181 accctctccc cagaggggcg aggggaccga ttgtgctcaa tattgaatat tgcgctcgtt
    12241 ttctccctct ccccattggg gtgaggggcg atgcctgctc catacccaac ctcatcgccc
    12301 atactcatct tccattctcc gctcttcatc ctccagttgc cgacgctcct gatcaagctg
    12361 gcgctggcga tcgtccagct gcctgcggcg atcttcaaac tggcggcggc ggtcgtcata
    12421 ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg tcgtcgcgcg aatcttcata
    12481 gtctcgccca cggtcagggt tataagcgtc attaatcgcc tgctgaatat taccaatagt
    12541 atcgtcgata atatcagcct gggccggaac ggtggacagc gtgagcaggg tgaataaaag
    12601 aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa actctatcca cattagtcat
    12661 tactcatcca tgcaagtagt ggatgaatct caatttctcc gctgctctat tgccgtaatc
    12721 gcctccacgc gttgttgatg acgaccgcct tcgtactgtg cgcccagcca cgcatccaca
    12781 atcatttttg ccagttcgag gccaaccact cgtgaaccaa aagccagtgc aacttcactg
    12841 gcataatgcg gataatcagt gcgctctgac gacctggtgt ctttatcaat cacctcactc
    12901 attgaccatg aataaaaggc tggcacagca acacagaatc ctgcggtgcc agcgacatga
    12961 ccgcccattg ctccggtacg ttttttaaaa agaaacaccg gttgcattga taaattgtgc
    13021 tcag
//
LOCUS       DAZFJF010000119        13014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFJF010000119 DAZFJF010000000
VERSION     DAZFJF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.119"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..1475
                     /gene="pitB"
                     /locus_tag="ACHKAU_003884"
     CDS             3..1475
                     /gene="pitB"
                     /locus_tag="ACHKAU_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO5532861.1"
                     /translation="MYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLA
                     VVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGT
                     WFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIVGLVI
                     AGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGA
                     NDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQHPELPQKLI
                     AMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSVSQRSQLRRI
                     MLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMIG
                     WRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMV
                     VDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1524..2216)
                     /gene="yghT"
                     /locus_tag="ACHKAU_003885"
     CDS             complement(1524..2216)
                     /gene="yghT"
                     /locus_tag="ACHKAU_003885"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5532862.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AIMDFMTARGYH"
     gene            2390..3103
                     /gene="yghS"
                     /locus_tag="ACHKAU_003886"
     CDS             2390..3103
                     /gene="yghS"
                     /locus_tag="ACHKAU_003886"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5532863.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3135..3893
                     /locus_tag="ACHKAU_003887"
     CDS             3135..3893
                     /locus_tag="ACHKAU_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532864.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            3939..5288
                     /locus_tag="ACHKAU_003888"
     CDS             3939..5288
                     /locus_tag="ACHKAU_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO5532865.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAGRELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5288..6112
                     /locus_tag="ACHKAU_003889"
     CDS             5288..6112
                     /locus_tag="ACHKAU_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532866.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6124..6684
                     /locus_tag="ACHKAU_003890"
     CDS             6124..6684
                     /locus_tag="ACHKAU_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5532867.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6715..7785
                     /gene="lptF"
                     /locus_tag="ACHKAU_003891"
     CDS             6715..7785
                     /gene="lptF"
                     /locus_tag="ACHKAU_003891"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5532868.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7782..8861
                     /gene="lptG"
                     /locus_tag="ACHKAU_003892"
     CDS             7782..8861
                     /gene="lptG"
                     /locus_tag="ACHKAU_003892"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5532869.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(8897..10069)
                     /locus_tag="ACHKAU_003893"
     CDS             complement(8897..10069)
                     /locus_tag="ACHKAU_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5532870.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10069..10317)
                     /locus_tag="ACHKAU_003894"
     CDS             complement(10069..10317)
                     /locus_tag="ACHKAU_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5532871.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10349..11263)
                     /locus_tag="ACHKAU_003895"
     CDS             complement(10349..11263)
                     /locus_tag="ACHKAU_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5532872.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11260..12948)
                     /locus_tag="ACHKAU_003896"
     CDS             complement(11260..12948)
                     /locus_tag="ACHKAU_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO5532873.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3102 a         3229 c         3401 g         3282 t
ORIGIN      
        1 atatatacac agggcttttg ttattgcttg ctctggcatt tgtgttgttc tacgaagcaa
       61 tcaatggttt tcatgacacg gcgaatgcgg tggcaaccgt tatttatact cgtgccatgc
      121 aaccacaact tgctgtggtg atggcggcat tttttaactt ttttggcgtg ttattgggcg
      181 gacttagcgt tgcctatgcc attgtccata tgttaccaac cgatttgttg ctgaatatgg
      241 ggtcaaccca cggtctggcg atggtctttt ccatgctgct ggcggcgatt atatggaacc
      301 tggggacgtg gttcttcggt ttaccggcct ccagttcgca caccttgatt ggcgcgatta
      361 tcggcatcgg tttaaccaac gcgctgttaa ccggctcatc ggtgatggat gcgttaaacc
      421 tgcgtgaagt gaccaaaatt ttctcctcgc tgattgtttc ccctatcgtc ggcctggtca
      481 ttgcgggtgg tctgattttc ctgctgcgcc gtttctggag cgggacgaaa aagcgtgacc
      541 gtattcaccg cattccggaa gatcgcaaaa agaaaaaagg caaacgtaaa ccgccattct
      601 ggacgcgtat tgcgctgatt gtttccgctg cgggcgtggc gttttcgcac ggcgcgaacg
      661 acgggcagaa agggatcggc ctggtgatgc tggtactggt ggggattgcc cctgctggct
      721 tcgtcgtcaa tatgaacgcg tccggctatg aaattacccg tactcgcgat gccgtctcca
      781 acttcgaaca ctatctgcaa cagcatcctg aactgccgca gaagttgatt gcgatggaac
      841 ctccattgcc tgcggcatcg actgatggcg cgcaagtaac agagtttcac tgtcatccgg
      901 caaatacctt tgatgcgatt gcgcgcgtta aaacgatgct gccaggcaat atggaaagtt
      961 acgagccgtt aagcgtgagt cagcgcagcc agctgcgccg cattatgctg tgcatctcag
     1021 atacctccgc gaagctggcg aaactgccag gcgtcagtaa agaagaccag aacctgctga
     1081 aaaaactgcg cagcgatatg ttaagcacca ttgagtacgc tccggtgtgg atcatcatgg
     1141 cggtagcact ggcgctcggc attggcacca tgattggctg gcgtcgtgta gcgatgacca
     1201 tcggtgagaa gattggtaag cgcggcatga cgtatgcgca aggcatggcg gcacaaatga
     1261 cggcggcagt gtctatcggt cttgccagtt atattgggat gcccgtctcc acaacacacg
     1321 tcctctcgtc tgcagttgca gggacgatgg tggtggacgg cggtgggtta cagcgtaaaa
     1381 cggtaaccag catcctgatg gcgtgggtat ttactttacc ggcggcgatt tttctttctg
     1441 gtgggctgta ctggatagca ttgcagttga tttaaccttc ctgaaaatgc ccggtcctgg
     1501 cgatcgggca tttccatttt tggctagtga taaccacgcg cggtcataaa atccataatc
     1561 gctttttctg catcaaccaa cacctgttcc agtggctgat tggcatcgat atcaaccagt
     1621 tgtgcaccac caaaggttaa ctgtggcgtt atggctatct ttcttgccag cgactccctt
     1681 ttgtggtcag gcttacgcgc gcaggcaact tcaaggtcaa cattgagttt gatgaccaga
     1741 tcaggcttat ggctcgccat ccagtgaaac gctttacgtt cctggcttgc cagccatgag
     1801 acaaaacgac caccttcaac gttaggtggg aacaccgtac catcgtaagc gccaggaatt
     1861 tggtcctgag gataacggtc ggttagaaca attaacccgc gacgacgaca ggcaagcata
     1921 tgacgaaagc gcagtaagcg acgggcgaca aacgctgtaa ttaccagcgc cggaactggt
     1981 cctggcaatt tttttgctgt tttcacctga tttcgttcaa ttgttttatg taaggatttt
     2041 cccatcaacg gtaattttgt cactgcacga ccgacatttc cggcttgttt tcctaaatga
     2101 actctttcgg cagcaccata tttttcgaca acggtaataa gatgttcaca caccgttgac
     2161 ttgcctgaac catcgctacc aataacggca attaatggag gtgttattga ctgcatgaat
     2221 atatccttaa atttaaatat ccatccaaaa tatttatttg gttaatatgt ttttatgaaa
     2281 gcgtaattca ggtcaatgtc acaattaacc attgtcacaa taaggttgaa cggatattct
     2341 gagcacggac cacgtaatat tcaatacatt atttactgcc gggtttttcg tgaatcaacg
     2401 taatatgtca ataattaatt ccacgccagt gcgtgttatt gccattgtag gatgtgatgg
     2461 ttcaggtaaa tcgaccctca cggcaagcct ggtaaatgaa ctggcagcaa gaatgccaac
     2521 agaacgcatt tatctcgggc aatcgtccgg gcgaattggc gaatggattt cacagctccc
     2581 tgttattggc gcaccttttg ggcgttatct gcgaagtaaa gcggcacatg tgcacgaaaa
     2641 gccctcaaca ccgcctggca atattactgc gctggttatc tatctgcttt cctgctggcg
     2701 ggcgtacaag tttcgcaaaa tgttgtgtaa aagccagcaa ggctttctgc tcatcaccga
     2761 ccgctacccg caggttgaag tgccgggttt tcgctttgat ggcccgcaat tggcaaaaac
     2821 cacgggcggt aacggttgga taaaaatgtt aaggcagcgc gagctgaagc tgtaccaatg
     2881 gatggcatct tatttgcccg tattgttgat tcgtcttggc attgatgaac aaaccgcgtt
     2941 tgcgcgtaaa cctgaccacc aactggcagc gttacaggaa aaaatcgccg ttacgccgca
     3001 actgacattc aatggcgcaa agatccttga actggatggg cgacaccccg ccgatgaaat
     3061 tctgcaagcg tcactacgcg caattcacgc cgcgctttcc tgatcaccaa aatggatagg
     3121 ggatgattta acgaatggat gcactacaaa ctcaaactgt taatagtaca accgcgccgc
     3181 agcccaacta cattccgggg ctgattgcgg tggtcgggtg tgatggcacc ggtaaatcca
     3241 cactgaccac cgacctggtg aaatcgctgc aacaacactg gcaaaccgag cggcgctatc
     3301 tggggctgct ctccggcgaa gacggcgaca aaatcaaacg attgccgttg gttggcgtct
     3361 ggctggaacg gcgactggcg gccaaatcct cgaaaaccca aagcatgaaa accaaatctc
     3421 cggcgctatg ggcggcggtg attatgtact gcttctcgct gcgaagaatg gcgaatctac
     3481 gcaaggttca gcgactggcg caaagtggcg ttctggtggt cagcgatcgc ttcccgcagg
     3541 ctgaaatttc gggcttttat tatgatggac cggggattgg cgtcgaacgt gcgaccggga
     3601 aaatcagcat gtttctggcg cagcgcgaac ggcgtttata ccaacaaatg gcgcaatatc
     3661 gcccggaatt aattattcgc ctgggcattg atattgagac tgccatctcc cgcaagcctg
     3721 accatgacta tgccgagctg caggacaaaa tcggcgtcat gtcgacgatt ggctataacg
     3781 gcacaaaaat tcttgagatt gattccagag cgccctacag cgaagtgctg gaacaggcac
     3841 agaaggcggt ttctctggtt gccatcgttt ctgaccgccg aagtttaact taaggctggc
     3901 gggatacttt ttactatcaa cgataaggtc aggctgaatt ggcgggtttt aacatcaaac
     3961 attggtttgc agatggcgcg tttcgcacca ttattcgcaa tagcgcctgg ttaggctcca
     4021 gtaatgtcgt gagcgccttg ctgggtctgt tggcgctctc gtgtgccggt aaagggatga
     4081 cgcccgccat gtttggcgta ctggtgattg tgcaatcgta cgccaagtcg atcagcgatt
     4141 ttattaagtt tcagacatgg caactggtgg ttcagtacgg aacgccagcc ttgaccaaca
     4201 ataatccgca gcaatttcgc aatgtcgtct cattttcctt ctcgctggat atcgtcagcg
     4261 gcgcggtggc gattgtcggc ggcattgcct tactgccatt cctttcccat tcattaggtc
     4321 tggatgacca aagcttttgg ctggcggcgc tgtattgcac gctcattcct tcaatggctt
     4381 cctccacgcc gaccggcatt ctgcgtgcgg tagatcgctt cgatttaatt gctgtacagc
     4441 aggcgacgaa accttttctg cgcgcagcgg ggagcgtcgt agcctggtat tttgactttg
     4501 gttttgcggg ttttgttatt gcctggtacg tgtcgaatct ggttggcggc accatgtact
     4561 ggtggtttgc agggcgcgaa ttacgccgca gaaatatcca taatgccttc aaattgaatc
     4621 tgtttgagtc tgcccgacac atcaaaggcg cgtggagttt tgtctggtca accaacattg
     4681 cccactccat ctggtcggcg cgtaactcgt gcagcacagt gctggtgggg atcgtgttag
     4741 gacccgctgc cgccgggtta tttaaaatcg ccatgacatt cttcgacgcc gccggaacgc
     4801 cagcgggttt gctgggtaaa agtttttacc cagaggtgat gcgtttagat ccgcgcacca
     4861 ccagaccgtg gttgttaggt gtgaagtccg gtttactggc gggcggaatc ggtattctgg
     4921 tggcgctcgc ggtgtttatc gtcggcaagc cgctcatttc gctggtgttt ggcgttaagt
     4981 atctcgaagc gtatgacctg attcaggtga tgctgggcgc aattgtgatt tcgatgctgg
     5041 gcttccctca ggagtcattg ctattgatgg cgggaaaaca gcgcgctttt ctcgtggcgc
     5101 aaaccatcgc ctcaatcggc tacatcgtat tgctgttcat gttctgtcat ctgtttggtg
     5161 tgctgggagc agcgtttgct tacttcggtg ggcaatgtct ggacgtggcg ctctcgttaa
     5221 ttccaacctt aaaggcattt tttcagcgcc attccttgct ttataacgca gccggagaga
     5281 aatcctgatg atgagaaaat atttcccgct tgaggcgagc gagcgtctat ttgttgcgat
     5341 tgaagaagat gatgtcgttg atgcacaggt tagtttgccg ccgactattg cactaagttg
     5401 taccaccgaa atcattcatg ataattacgc gttatgcctg caattctggc tcaatggcgt
     5461 tgatcgtcag gaattactgc gtttagttcg caaacaagcc aaaggcgatg agctgactgc
     5521 tgatgagcgc aaacaattta aatacatgcg agcgcgctac aagcacctgc gttttgcgca
     5581 acggctgtat ttaaagaagc atcaggccgg atttttattc ggcaaaacga ccgtttttct
     5641 ggggcgtttt caggacggct ttcgtaacgg taagaaaaat attgtgtcgt attacggcaa
     5701 cctgttacgg atttatttaa gctctccggt ctggtcgttg gtgaactatt cattacgcca
     5761 tagccagtta gaaagcgtaa gcagttttat tgcctatcgg caaaagcaaa tgcataccct
     5821 gaaagagatt attgccaaac cgcgactaac cggcagggaa tttcatgatg tgcgaaaaat
     5881 catcagccaa caggtttctt attacgacac cttaagatcg cttgatcctg aaaataaaga
     5941 ggcgttacag atttcccgtt tcctcgccgc cattaatggc ctgatgggag ataaacacga
     6001 cgacatggtg gcggatgata tggaaaaccg ccagtcgtat gatgcaccgt tggcgctgga
     6061 tagcgatatt cgtcagcgct tagagctgct gatttcgcgt tttccgctat agccggagca
     6121 atgatgtcat ctcgtttgat tattgcgttg attatcatgc tcttagcccc gggtgtgcag
     6181 gcacacaatt tcgtcactgg caaaacggtt acccctgttt atatccagga aggcggcgag
     6241 ctgcttctga atagcgacga cgaaattcac taccaaaagt ggaacagcac acaattagca
     6301 ggaaaagtcc gcattattca gtatatcgcc gggcgtaagt cggcgaagaa aaagaactca
     6361 ctgttaatta aagcggttga agcggcaaac tttccgcagg atcgttttca gcccaccacc
     6421 attgtgaaca ccgatgatgc catctttggt actggctatt ttgttgttgg caaaatcgaa
     6481 aaaaataaac gccgctatcc gtgggcgcaa ttcattattg acggcaatgg acagggacgc
     6541 gttgcctggc gcttgccgga gcagagttca accattctgg tgctgaataa agccgggcaa
     6601 attcagtggg caaaggacgg cagcctgacg ccggaagagg tagatcatgt cattgcgctg
     6661 gcgcagaaac tgatcaatga ataaaaaatt caaaggattg agggcgttag tgagatgaag
     6721 ctggttgaac actacatcat gcgtggtacg cgccgtctgg tgctgattat cgtcggtttt
     6781 ctgatcttta ttttcgccag ctactccgca cagcgttatc tgaccgaagc ggcaaatggg
     6841 acattagctc ttgatgttgt gctggatatc gttttctaca aagtgctgat tgcactggag
     6901 atgttgttac ctgttggtct gtatgtgtca gttggcgtga cgctagggca gatgtatacc
     6961 gactcggaaa ttaccgctat ctctgcggcg gggggcagtc cgggacgctt gtacaaagcc
     7021 gttctttatc tggcgatacc gctaagtatt tttgtcaccc ttctgtcgat gtatggtcgt
     7081 ccgtgggctt atacgcagat ttatcaactg gagcagcagt cgcagtcgga gctggatgtt
     7141 cgccagttgc gggcaaagaa atttaacact aacgataacg gacgaatgat cctttcgcag
     7201 acggttgatc aggataataa tcgcctgact gacgcgctga tttatacttc tactgccaat
     7261 cgaacccgca ttttccgcgc ccgttcggtt gatgtggttg acccatcacc cgagaaaccg
     7321 accgttatgt tgcacaacgg cactgcctat cttctcgatc atcaggggcg tgacgacaac
     7381 gaacagatct accgtaatct gcaattacat ctgaatccgc tggatcaaag ccctaacgtc
     7441 aaacgcaaag caaaatcggt cacggagctg gcgcgctccg cctttcctgc cgatcatgcc
     7501 gaactgcaat ggcgacaaag ccgtggcctg acagcattgt tgatggcgct gctggccatt
     7561 tcattaagtc gggtaaaacc gcggcaaggg cgattttcaa cattattgcc actgacgttg
     7621 ctgtttattg ccatttttta tggcggcgac gtctgccgta cgctggtggc taacggcgcg
     7681 attcccctca ttcctggttt gtggttagta cccggactca tgctaatggg cctgctgatg
     7741 ctggtcgcac gcgacttctc tttgctgcag aaattttccc gatgaatgtt ttcagtcgct
     7801 atttaatccg tcatctcttt ctcggttttg ccgccgccgc agggctattg ctgccgcttt
     7861 ttaccacctt caacctgatt aacgaactgg atgatgtcag cccgggcggt tatcgctgga
     7921 ctcaggcggt gctggtggtg ctaatgacct taccgcgcac actggtcgaa ctttcgccgt
     7981 ttatcgcgtt attgggtggt attgttggac tggggcagtt atcgaaaaac agtgagctta
     8041 ccgccattcg cagcacgggg ttttctatct tccgtattgc actggtggtg ctggttgccg
     8101 ggatattgtg gactgtttcg ttaggcgcga tagatgagtg ggtggcgtcg ccattgcagc
     8161 aacaggcgtt gcaaatcaaa tcgaccgcca ccgcgttggg ggaggacgat gacattaccg
     8221 gcaatatgct gtgggccagg cgtggtaatg aatttgtgac ggtgaaatcg ctgaacgagc
     8281 agggccagcc ggtgggcgtg gagatatttc attatcgcga cgatctctcg cttgaatcct
     8341 acatttatgc acgcagtgcc accattaaag acgacaaaac gtgggtcctg catggtgtga
     8401 atcataaaaa atggctgaat ggcaaagaaa cgctggaaac attagataat cttgcctggc
     8461 aatcggcctt caccagtatg aatcttgaag agttatcaat gccggggaat actttttctg
     8521 tccgtcagct taatcattac atccattatt tgcaggaaac cgggcaaccc agcagcgaat
     8581 accgccttgc actgtgggaa aaactggggc aacccatcct gaccctggcg atgattttgc
     8641 tggctgtgcc gtttaccttt actgccccgc gttcgcctgg gatgggtagc cgtctcgctg
     8701 taggcgtcat cgtgggctta ctcacctgga tcagctatca aatcatggtt aatctgggat
     8761 tgctatttgc gttaagcgca cctgttaccg cgctcggttt accggtagcg tttgtgttgg
     8821 tggcgttgag cctggtgtat tggtatgaca gacaacatta aacccgccgt ggcgcaaaca
     8881 ccacggcggg ctcacattac tgcttcagag tagcaaatgc ctgaatgatg gcatcgattt
     8941 gctcacgact gtgggcagca tttacgctac agcgcagcag cgtgatgcct gcaggcgcgg
     9001 caggcggcag gataagattc acatacacgc ccagcgagat aagctcgcgc cagatgcgta
     9061 aaccttcctc tttcgaaccg attattactg gcactaccgg gctaatatgt gaacttaact
     9121 cataacccag tttttgcaga ccgtcataca gatggttggc gttatccatc agcttttgcc
     9181 gcagctctgg ttgtgtggca atagttctta acgacgagcg cacggtcgca atgcaggacg
     9241 gagaaggaga ggcggtaaaa atatacgggc ggctgctgta gcgcagcact tccatcgctt
     9301 ccgaaccgac agcaaaaccg ccaatcgagg ccagactttt actgaaagta ccaacgataa
     9361 tatccacatc atcttcaacg ccgacagctt cagccagtcc gcgccccgtt gcgcctaaca
     9421 cgccaaacga atgcgcttca tcgacaatca aataaccacc gagacgacgt ttgatatcaa
     9481 caatttccgc cagcggcgca acgtcaccga gcatgctgta gatgccctca acgatgataa
     9541 tcgcctcttt cgcccgttca ccgaggcgaa ccatgcgccg ttccagatct ttagcatcgt
     9601 tatggcggaa acgaataatt tccgcgccac cgagagaaca ggcgtcataa atactggcgt
     9661 ggctgtcggc atccagtaac accacggcgt tatggtcggc aagtgcgctg atgacgccta
     9721 aattagcggt ataaccggtt gaaaaaacga tagcggtagg gcgattgaaa aactcggcaa
     9781 tctctttctc taacgccagg tgcggcgcat aactgccgtt tgccatacga gaaccggtgg
     9841 tgccggtgcc ttgggccgct aacgctgcct gaccttcgct gatggcatca tgattaaagg
     9901 ttaaaccgag ataattattg gtcccggcca gaatcacttt tttattgccg atccggcctt
     9961 cggtggcgga atacacttca tcaatacagg tgccaaacgg tgttaacccg ttgtcactaa
    10021 attgcagtcg ctcgccagca aggcgtgcat atttatcgta tagccccatt atttattctc
    10081 cagccatgga agtagagttt ccattaattg cgcaggggtt ttgacatcca gcagaatatt
    10141 aataggaatg gaaatatcga atctgtcttc cagcatcatt aagagatcca tcactttgat
    10201 cgactccagg ccaagatcgt tgacgagatc gctgtcgggc ttaatttcca caccgttttc
    10261 gaccaaatcc tgtaaacagg aaaggatgta atccattact atttcacgat ttaccataga
    10321 aacgttaaca cacctttaaa caagacaatc aaaatagccc gttgcgtaat gcgtgttcca
    10381 ggctaatccc gggaaaccaa ttaatatctt cagaaatacg gttattactt gccgaccagt
    10441 cggcgtgggt taattcacga attttcgagc gggtcagcat aggttcttta cctgccaggc
    10501 gactcaacgc ggtactgata tccgcaaggc aggtgagtac cggcagagga ataccaacca
    10561 ttcgcacgga accgcaacgg acgttggcgg caagttgctg tatgcgttgc cagtcatagc
    10621 cgccagcgac gctatcgcat aattcatagg tttgcgtctg tacagtttcg gcgcttaacc
    10681 actgacccac tgcttgcgca aaatcggtga cgtgcaggaa agagagctgt gtatctggtg
    10741 caccaagtcg tggcagcagg ccgcgcagca tccagtcaaa cagcggtttt aactctttat
    10801 cgccgggacc atacacggct gtcgggcgaa aaacgcccag cgtaatttcg tcagccattg
    10861 cagtcagccg ttgttcggcg atgtgtttgg aatttgcgta ccaggagagc tcgggatggc
    10921 gcgccgccag cgaagagata aacagaaaac gttggcaaaa gcagctctct tttgctgctt
    10981 gcatcaggcg caggctgccg tcaacgttac agcgggtgaa aatctcttct ttgtgcccgc
    11041 gcacttgccc ggcgcaatgg accactgcgc tggctccggc aaccagcttg ctaagtgaat
    11101 gagtatcttc cagcgaaccg cgcacccagg tgagattatc attgacgtga gcgcgggcag
    11161 tacgcgtcaa tgcgcgaacg tgaaagccgc gggcgagcag gttatcgata atatatttac
    11221 cgataaaccc ggtagcgccc gtcaccgcga cagtttgatt catgccaggg attcctgcac
    11281 attaagactg gcagcataag ccttctgata acgttttttc gcttccgcac gggcaggctt
    11341 gccggaggac gttcggggaa tactgtgggg cggcaacaga tcgatagccg cggtcacgcc
    11401 aaattcgctt tggatccgtg ccgccagcgc gtggataagc tgcccgcgac gttcttcgtc
    11461 gctgatccga cactggatct gcaaaatgat tttttcctgg gcggtaacaa aagcaatcgc
    11521 atcgccagaa tgaatttccg gttcttgttc cgcaatatat tcaatatcct gcggccagat
    11581 attacggcca cgaataataa tcagatcttt aatgcgtccg gtgacataca gataaccgtc
    11641 cagcagataa ccgaggtcgc cggtgtctaa ccagcccgtc gcggcaatct cgtcttgcga
    11701 aacctggtcg ccaaagtaac cgctcatcag gctggggccg gagatgcaaa tatggcctac
    11761 cacacgttcc gcgaccggca tacctgcttc attgcggatt tcaatgccat gttccggcaa
    11821 cgctttgccg cagttgacga aagtcgatac ggcgcgtgtc tctgcacccg gcgcgacggc
    11881 tttaccctga tattcgagga tgtcgcgatc cacttcgtta accacaaccc cggaggcttc
    11941 atcagagaag ctgacagcca gcgcattttc tgccagtccg tagcacggca tgaaagtttt
    12001 attgtcaaag ttaacctgac ggaaacattc agcgaattga tggagttgtt ctgcggagat
    12061 gggttctgca ccaataccag cgacgcgcca gcaggaaaga tccagttcag cgagatcttt
    12121 ttcattcacg cggcgctggc acaattcata gccaaacggc ggcgcaacgg aaacggtgcc
    12181 gcgattttta ctgatcaatt taagccattg cagaggacgc atggcaaaat cctgagtgcg
    12241 caaataatct actgaaagct gcgtggcgac ggggggcagg agaaagccga ccagtcccat
    12301 atcatggtag aaaggcagcc aggagacgca gcggtcgcca gggcgtaatt taatgccgtc
    12361 gtggcttata gcacgtagat tagccatcac ttcgcgatgg gtgataatga cgccacgggg
    12421 aaaacgggtg ctgccggagg tgtactggag gtaggcgata tcgttcggaa ctggacgctg
    12481 gagcgcaaca tcggcttccg gtaatgcctt aaaccaggcg tggcttaaaa catgtaattc
    12541 ggggttgtta tgcgtcgcgg cattgaccag tggcaaccac tcatcaccag tgataatggc
    12601 tgcgggctgg caacttgcca gtaaaccctg gagtttggcg ctccaggaat cccgctgacc
    12661 gacgcccatt ggaatcgcca acgggacggc gactaagcca gcatactgac aggcaaaaaa
    12721 agcctctaca aacccgctac tcgtttcggc aatcagcgcc acgcgttcgc cttttttcag
    12781 gttcagcgat aacaaccgct tcgcaccagc ttcggcacgc gtttttaatg tctgatattc
    12841 cagttgatct tcaagttggc aacgtctgtc ataaaaattc attccggcgc tactcagagc
    12901 ggcgtagtcc aaagcatcaa ccagcgttgg aaaatcggca tagcgcatag gtagggaatg
    12961 cgtaaagatt ttattagaca tatacaccac gaaaaaattg gatatcaaaa ataa
//
LOCUS       DAZFJF010000120        12961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFJF010000120 DAZFJF010000000
VERSION     DAZFJF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.120"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..769
                     /locus_tag="ACHKAU_003897"
     CDS             <1..769
                     /locus_tag="ACHKAU_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5532874.1"
                     /translation="TLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDT
                     SRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKL
                     SIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSK
                     IKNSLQMRIDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVD
                     PSGHGTINMRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(812..1672)
                     /gene="rsmI"
                     /locus_tag="ACHKAU_003898"
     CDS             complement(812..1672)
                     /gene="rsmI"
                     /locus_tag="ACHKAU_003898"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO5532875.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1736..3772
                     /gene="lpoA"
                     /locus_tag="ACHKAU_003899"
     CDS             1736..3772
                     /gene="lpoA"
                     /locus_tag="ACHKAU_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO5532876.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3730..4125
                     /locus_tag="ACHKAU_003900"
     CDS             3730..4125
                     /locus_tag="ACHKAU_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO5532877.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4145..4735
                     /gene="diaA"
                     /locus_tag="ACHKAU_003901"
     CDS             4145..4735
                     /gene="diaA"
                     /locus_tag="ACHKAU_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO5532878.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            4745..5320
                     /gene="dolP"
                     /locus_tag="ACHKAU_003902"
     CDS             4745..5320
                     /gene="dolP"
                     /locus_tag="ACHKAU_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5532879.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5434..6474)
                     /locus_tag="ACHKAU_003903"
     CDS             complement(5434..6474)
                     /locus_tag="ACHKAU_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5532880.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSLGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6547..7182)
                     /locus_tag="ACHKAU_003904"
     CDS             complement(6547..7182)
                     /locus_tag="ACHKAU_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO5532881.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            7310..7828
                     /gene="yhbO"
                     /locus_tag="ACHKAU_003905"
     CDS             7310..7828
                     /gene="yhbO"
                     /locus_tag="ACHKAU_003905"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO5532882.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7808..8251)
                     /locus_tag="ACHKAU_003906"
     CDS             complement(7808..8251)
                     /locus_tag="ACHKAU_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO5532883.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            8302..8604
                     /gene="yhbQ"
                     /locus_tag="ACHKAU_003907"
     CDS             8302..8604
                     /gene="yhbQ"
                     /locus_tag="ACHKAU_003907"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO5532884.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8591..9094)
                     /locus_tag="ACHKAU_003908"
     CDS             complement(8591..9094)
                     /locus_tag="ACHKAU_003908"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5532885.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9088..9612)
                     /locus_tag="ACHKAU_003909"
     CDS             complement(9088..9612)
                     /locus_tag="ACHKAU_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO5532886.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9821..10816
                     /locus_tag="ACHKAU_003910"
     CDS             9821..10816
                     /locus_tag="ACHKAU_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO5532887.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10825..11703
                     /locus_tag="ACHKAU_003911"
     CDS             10825..11703
                     /locus_tag="ACHKAU_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO5532888.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11909..12916
                     /locus_tag="ACHKAU_003912"
     CDS             11909..12916
                     /locus_tag="ACHKAU_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO5532889.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         3128 a         3560 c         3377 g         2896 t
ORIGIN      
        1 cacattacag gcgaatgaca ttccaggact cgtgactgac cagaccgttt ctgttaacac
       61 ccgattcaca cagataaaaa gcaacaccgt atattctgct gcaacccaaa cgggtgtttg
      121 ccagggtgac acgtctcgtt atggacccgt taatattggt gcaaatacca cctttaccct
      181 gtatgtcacc aagccatttc tcggctcgat gaccattccg aaaacggata ttgccgtcat
      241 taaaggcgca tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc atgatttagt
      301 caagttatcg attcagggaa atctcaccgc cccacagtcg tgcaaaatta atcagggcga
      361 tgttattaag gttaattttg gattcatcaa tggtcagaag tttaccaccc gcaatgccat
      421 gccagacggt tttactccag tagactttga tatcacttat gactgtggtg atacttcaaa
      481 gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag accagtacaa
      541 cctggtcgcc agacgaagaa gttcagacaa tgcgcccgat gtcggtattc gtattgaaaa
      601 tctcggcggc ggagttgcaa atattccttt tcagaacggt atccttcccg ttgatccttc
      661 cgggcatggc accatcaaca tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga
      721 aacaggaaaa tttcagggca ccgccaccat taccgtcatc gtgcggtaaa agaagacgtt
      781 gcgttttgat taacgcaacg cttcggcact cttacccctg ctgctccagc gcatacttat
      841 acagcgcatt tttcttcacg ccgtgaattt ctgccgccag cgccgccgct tttttcaacg
      901 gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct
      961 gtgctttatg accttcgata atcagcacca tttcgccttt gcgacggttt tcatcttctt
     1021 ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag gttttggtca
     1081 gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata tcttccaggc
     1141 tatctaacag acggtgggtg gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg
     1201 cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttcg taacagaaac
     1261 ggtcagaggg taaacccgcg gcgcttaacg cagtgatagc agcacacggc ccgggtagcg
     1321 gcaccacgca aatccccgct tcacggcagg tacgcaccag atggtagcca ggatcgttaa
     1381 ttagcggcgt tccggcatcg gaaaccagcg caatgttctg cccttcttgc agcttcgcca
     1441 gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa
     1501 tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca atcagatcaa
     1561 cggcctgtaa tacctctaac gcacgctggg tgatatccgc cagattgccg attggcgtcg
     1621 gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc attgtgtcgt
     1681 ccgtattgcc gatttaatat tgagcattgc gtaaaaaaat atcactggat acattatggt
     1741 accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct
     1801 gattttcgcc ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac
     1861 ggcgcaggct gattctgcct tttatcttca gcagatgcag caaagctctg atgataccag
     1921 gatcaactgg caattactcg ccattcgtgc actggtgaaa gaaggtaaaa ccgggcaggc
     1981 ggttgagttg tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac
     2041 actgctggcg gtagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct
     2101 ggcgaaaatc acacctgccg atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa
     2161 aatcgatgcc agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga
     2221 accgctgctc ggcgcgaaag aaaaacagca gaatattgat gccacctggc aggcgctctc
     2281 ctccatgact caggaacagg cgaatacgct ggtgatcaac gccgacgaaa atattctgca
     2341 aggctggctg gatctgcagc gcgtctggtt tgataaccgt aacgatcccg acatgatgaa
     2401 agccgggatc gccgactggc aaaaacgtta tccgaacaat ccgggcgcga aaatgctgcc
     2461 aacgcagttg gttaacgtaa aagcgtttaa accagcctcg accaacaaaa tcgccctgct
     2521 gttgccgctg aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc
     2581 ggcgaaaaat atcggcactc agccagtggc ggctcaggta gctgccgcac ctgccgcaga
     2641 cgtagctgaa caacctcagc cgcaaaccgt ggatggcgtt gccagcccgg cacaagcctc
     2701 ggttagcgat ctgaccggtg aacagcctgc ggcacagcca gcgcctgtaa gcaccccggc
     2761 agcaaccacc acagcggtaa gcgcgccagc gaatccatcc gcagaactga aaatctacga
     2821 tacctcatca caaccactta gccagatctt aagccaggtt cagcaggatg gcgcgagtat
     2881 tgtggttggt ccgttgctga aaaataacgt tgaagagttg cttaagagca acaccccgct
     2941 gaacgtgctg gcactgaacc agccggagaa tatcgaaaac cgcgtcaata tttgttactt
     3001 cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa
     3061 acaagcgccg ctggtgctga tcccacgcag ttcattgggc gatcgcgtag ccaatgcgtt
     3121 tgcgcaggag tggcagaaac tgggcggcgg caccgttctg caacaaaaat ttggttccac
     3181 cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatc gctttaacgg gtaccccgat
     3241 tactcccaga gcgacaaccg actccggcat gacgaccaac aatccaacgc tgcaaaccac
     3301 gccaaccgat gaccagttca ccaataatgg cggtcgtgtc gatgcggtgt acattgtggc
     3361 aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag
     3421 cggtgcaacg ctgtacgcca gctcccgcag tgcgcaaggg accgctggcc cggatttccg
     3481 actggagatg gaaggcttgc agtacagcga aatcccgatg ctggcaggcg gtaatctacc
     3541 gttaatgcag caggcactca gcgcggtgaa taacgattat tcactggctc gcatgtatgc
     3601 gatgggcgtc gatgcctggt cgctggcaaa tcatttctca caaatgcgcc aggttcaggg
     3661 ttttgaaatc aacggtaata ccggaagcct gacggcaaac ccggattgcg tgattaacag
     3721 gaagttatca tggctacagt accaacaagg tcaggtagtc cccgccagtt aaccaccaaa
     3781 cagaccggcg atgcgtggga agtacaagcg cgtcgctggc tggaaggcaa aggactgcga
     3841 tttgtcgccg ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc
     3901 cggaccaccg tttttgtcga ggtacgctat cgccgctctg cgctttatgg cggcgcggca
     3961 gccagtgtga cccgcagcaa acaacacaaa ttattacaga ctgcccgctt gtggctcgcg
     4021 cgtcataatg ggagttttga tactgtggat tgccggttcg atgtggtagc cttcaccggg
     4081 aatgaggttg agtggattaa ggatgccttt aatgaccact cataattaag gtttaaggat
     4141 tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc attcaaactc aaattgcggc
     4201 ggcagaggcg cttccggatg ccatctcccg tgcagccatg acgctggttc agtctctgct
     4261 caatggcaac aaaatcctct gttgtggtaa tggaacttcc gctgccaatg cacagcattt
     4321 tgctgccagc atgatcaacc gtttcgaaac ggagcggccc agcttacctg ccattgcact
     4381 aaatactgat aatgttgtct taacggcgat tgccaacgat cgcttacatg atgaagtgta
     4441 tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca tttccacccg
     4501 tggcaacagc cgcgatattg taaaagcggt agaagctgcc gttacgcgtg atatgactat
     4561 tgtggcattg accggctatg acggcggcga acttgcaggt ttgttagggc cacaagatgt
     4621 ggagatccgc attccttcgc atcgtagtgc tcgcattcag gaaatgcata tgctgacggt
     4681 aaattgcctg tgcgatctga tcgataacac gcttttccct caccaggatg tttaaggaga
     4741 atacatgaag gcattatcgc caatcgcagt ccttatttcc gcgctgctgt tgcaaggttg
     4801 tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa ctgacccacg
     4861 cagtgtcggc acccaggtgg acgatggtac cctggaagtg cgcgtgaaca gcgcattgtc
     4921 gaaagacgaa cagattaaga aagaagcccg tattaatgtc acggcgtatc agggcaaagt
     4981 gctgctggtt gggcagtcac caaacgctga actttcggct cgcgccaaac agattgctat
     5041 gggcgtagac ggtgccaacg aagtgtataa cgagattcgt cagggccagc cgattggtct
     5101 gggcgaagcg tctaacgata cgtggatcac caccaaagtg cgttcgcagc tcttaaccag
     5161 cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat
     5221 ggggctggtg actgaacgtg aagcgaaagc ggcggcagat attgccagcc gggtgagcgg
     5281 cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag cagccccttg taatgcctga
     5341 tgcgacgctt gccgcgtctt atcaggccta caacgatgca aaccgtaggt cggataaggc
     5401 gtttacgccg catccgacat gttttccctc aaatcagaac aacagcgcca gcccgccgac
     5461 aatcacacca gacactgcca ccatcgcccc tgtcagccat aaggctttcg ctgggaacgc
     5521 tttgcgcagc ataatcagtg acggcaaact caccgccggg agcgtcatca acaatgccag
     5581 cgccggagca gttcccatac ctgccagcat catcgtttgt acaatcggaa tttctgctgc
     5641 cgtgggaatg acaaacaagc atcctgctac cgccatcgcc atcacccaca tcaggctgtt
     5701 atcgacagca ccatcggcat gggggaataa ccagacgcga gcggcaccca acaccagtac
     5761 tgcaaggatg taaaccggga tcgtactcca gaaaagcgtc catagcgccc tgccccagcg
     5821 gctaaaaaaa ccgccctgtg cttccggtat gtcaatttcg accggtgcct gcgtttgcgg
     5881 tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca ccagcccggc
     5941 caccagacga atcgccgcaa aaccccagcc gaggacaaac cccataaaca ccagcgtcgc
     6001 cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccagcg acacctgttg
     6061 gcgacgcatt cccgccgcga ccggagccgc acagcaggta cacatcatgc ccggcagcga
     6121 aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac gcaacaacca
     6181 gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca ctgccgcttt
     6241 ccataccgcg aggaaataga gcatcgcgta atccaacgcc gcctgccacg gattagcatc
     6301 cgcctgcgca aggatagatt taccgatact gtgggtttcg gcagcagtaa acgctttgcc
     6361 gtagtaaggt tgccatttca cataccagag accggcaatg acaacgagaa agaaaagcgc
     6421 gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag tcatagcatt
     6481 ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat caattcttgg
     6541 gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca caccttcatg
     6601 cccgggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat caatggattt
     6661 ccagttaccg ggtaataaac ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt
     6721 gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc agttctgggc
     6781 gattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg agtgggcatt
     6841 ggcacccata gcactgacca ccagcatatg ctgcgcgccc agtcgccgcc cggttaatgc
     6901 ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc ccgcttctcg
     6961 ccgcgtggtg ccgagacaac aaaacacaat gtcgatggga tcggtgacct gcgccagcgc
     7021 gtcagtcagt tgcggatcat ggggattaaa caccccgggc atatcgccca acggacgtcg
     7081 cgtcggcgcg gctatggcgt taactttcgg ttcgttaatc aacatccgca gcaggtgacc
     7141 gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc ctttacagaa
     7201 ttgtctgcct tgcgctccac ggctcatgca ccaggcttaa tagaccatga ggtaattatc
     7261 cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa
     7321 aattgccgtt ttaatcactg atgaatttga agattcagaa tttacttcac ccgcagacga
     7381 gttccgtaaa gccggacacg aagtgattac cattgaaaaa caagcgggta aaacggtgaa
     7441 aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag tgacgccagc
     7501 ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc gtggcgacaa
     7561 ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt tcgccatctg
     7621 tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac tgaccgcggt
     7681 taaaccgatc attattgatg ttaaaaacgc gggcgcggaa ttttacgatc aggaagtcgt
     7741 ggtcgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag cgtttaaccg
     7801 cgaggcgtta cgcctgctcg gtgcctgagc cgcgtagcca aatcattttt ttaccaaagc
     7861 ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc ggggccgaca
     7921 gcattctggc aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg ctttcctcac
     7981 cttccagcct gcggatctca cctttaaact gcacgccgcg aattaacgct accgttttcg
     8041 gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc gcgtggcgcg
     8101 ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga taaaaagcat
     8161 tagcgcacca gagttcccct tcctgctgca cacaccaggt gacaacatgt tgttttgcca
     8221 accaacggct gatggtgatg agtgtttcca ttgctgttct cttttatact gtgggcctga
     8281 ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg taccgccgac
     8341 aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca gcaccaaagc
     8401 ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt ttctgcgcca
     8461 gtgggcgatc gttcgctggc gttacgggcg gaatatcgcg ttaagcaact gaccaaacgg
     8521 caaaaagagc gtctggtagc ggaaggcgca gggtttgcag agctgttaag cagtctgcaa
     8581 accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagacca gttacgccat
     8641 tcaacgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgcct ggccagcggc
     8701 aacgcaggtc gtgatgtgcc gccagttcaa agccaaaacg gctgtacagc gccggatcgc
     8761 ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct tcatagacca
     8821 gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt gccatgccga
     8881 cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag ccaatgacct
     8941 gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca cgcagatcgt
     9001 gaaccagctt cgcttccgca tcactttcga atgagcgacg tagcagggca tcaatacccg
     9061 gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg tctgtttggt
     9121 ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg
     9181 taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca catacagccc
     9241 cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat ccggatcttg
     9301 tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca catcagcttg
     9361 cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt gcaggtcaat
     9421 atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc catcatccag
     9481 cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg
     9541 cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac gggaacgcag
     9601 tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc catatcagaa
     9661 aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc accaatacaa
     9721 ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc cagttcgtta
     9781 acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc tctgccctgc
     9841 cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg tttatatcgg
     9901 gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg agaaaaaatt
     9961 gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg cgattaacac
    10021 ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc gtggatatgg cggcgcagct
    10081 gggtgccgac gcgctgatcc tcgccgacct cgccatgcta gagtacgccg ccgagcgtta
    10141 cccgcatatt gagcgtcatg tgtcagtgca ggcttcggcg accaatgaag aggcgattaa
    10201 cttttatcat cgccattttg acgttgctcg cgtggtgctg ccgcgcgtgt tgtcgattca
    10261 tcaggtgaaa caactggcac gggtcacacc tgtaccactg gaagtctttg ctttcggcag
    10321 cctgtgcatt atgtcggaag gtcgttgcta tctgtcgtcg tatctgacgg gtgagtcgcc
    10381 caacaccgta ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa cgccgcaggg
    10441 gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg aaaacgcagg
    10501 ttatccgacg ctatgtaaag ggcgttatct ggtggacggc gagcgctatc acgcgctgga
    10561 agaaccaacc agtctcaata ccctggaact gctgccggag ttaatggcgg cgaatattgc
    10621 ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg tggcgaaagt
    10681 ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcatac cgcaaagcgc
    10741 gtggatggag acgctcgggt cgatgtccga aggcacgcag accacccttg gcgcgtatca
    10801 ccgtaaatgg cagtgagaag agcaatgaaa tattccttag ggccagtgct gtggtactgg
    10861 ccaaaagaga cgctggaaga tttttatcag caggccgcca ccagcagcgc cgacgtgatt
    10921 tatcttggtg aagcggtatg cagcaagcgc cgggcaacca aagttggcga ctggctggag
    10981 atggcaaaat cgctcgccgg aagtggcaag cagattgtgc tctccacgct ggcgctggtg
    11041 caggcatcct ctgaactggg cgaactgaaa cgctatgttg agaacggtga gtttctgatt
    11101 gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc
    11161 gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa tattgctcaa acagggcatg
    11221 atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct gcttaatcag
    11281 tgcgatgagc tgggcattcg caaccagttt gaagtggaag tcctgagcta cggtcatcta
    11341 ccgctggcct actccgcccg ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag
    11401 tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca ggaaaaccaa
    11461 caagtgtttg tactcaatgg cattcagacc atgagcggct acgtttacaa cctcggtaac
    11521 gagctggcat ccatgcaggg gctggttgat gtggtccgcc tgtcaccgca gggtactgac
    11581 actttcgcga tgctcgacgc cttccgcgct aatgaaaatg gcgctgcgcc actaccgttg
    11641 acggcaaaca gcgactgtaa cggctactgg cggcggctgg ccgggctgga gctgcaagcc
    11701 taagtaaata tgctcgtcat acttcaagtt gcatgtgctg catctgcatt cgttcacccc
    11761 agtcacttac ttatgtaagc tcctggggat tcactctctt gtcgccttcc tgcaactcga
    11821 attatttaga gcacagctca ctttgttaac aactttaact actctttaat gcagtattaa
    11881 agattaatcg gtaacaaagt gagctgttat gactgataaa accattgcgt tttcgctact
    11941 cgatctggcc cccattcccg aaggttcttc agcgcgagaa gcattctccc actctctcga
    12001 tctcgcccgt ctggctgaaa agcgcggcta tcatcgctac tggctggcag aacaccacaa
    12061 tatgactggc attgccagtg ctgccacgtc ggtattgatt ggctatctgg cggcgaatac
    12121 caccacgctg catctggggt ctggcggcgt gatgttgcct aaccactcac cgttggtcat
    12181 tgccgaacag ttcggcacgc tcaatacact ctatccgggg cgaatcgatt tggggctggg
    12241 tcgtgcgcca ggtagtgacc aacggacaat gatggcgctg cgtcgccata tgagcggcga
    12301 tattgataat ttcccccgcg atgtcgcgga gctggtggac tggtttgacg cccgcgatcc
    12361 caatccgcat gtgcgcccgg taccaggcta tggcgagaaa atccccgtgt ggttgttagg
    12421 ctccagcctt tacagcgcgc aactggcggc gcagcttggt ctgccgttcg cgtttgcctc
    12481 acacttcgcg ccggatatgt tgttccaggc gctgcatctt tatcgcagca acttcaaacc
    12541 gtcggcacga ctggaaaaac catacgcgat ggtgtgcatc aatattatcg ccgccgatag
    12601 caaccgcgac gctgagtttc tgtttacctc aatgcagcaa gcctttgtga agttgcgccg
    12661 tggcgaaacc gggcaactgc cgccaccgat tcaaaatatg gatcagttct ggtcgccgtc
    12721 cgaacagtat ggtgtgcagc aggcgctgag tatgtcgttg gtaggtgata aagcgaaagt
    12781 gcgtcatggc ttgcagtcga tcctgcgcga aaccgacgcc gatgagatta tggtcaacgg
    12841 gcagattttc gaccaccagg cgcggctgca ttcgtttgag ctggcgatgg atgttaagga
    12901 agagttgttg ggatagtgtg tcttaacgcg ggaagcctta tccgagctgg caacgctgtc
    12961 c
//
LOCUS       DAZFJF010000121        12867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFJF010000121 DAZFJF010000000
VERSION     DAZFJF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.121"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            663..890
                     /gene="ykgL"
                     /locus_tag="ACHKAU_003913"
     CDS             663..890
                     /gene="ykgL"
                     /locus_tag="ACHKAU_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO5532890.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(925..1065)
                     /gene="ykgO"
                     /locus_tag="ACHKAU_003914"
     CDS             complement(925..1065)
                     /gene="ykgO"
                     /locus_tag="ACHKAU_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO5532891.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1065..1328)
                     /gene="ykgM"
                     /locus_tag="ACHKAU_003915"
     CDS             complement(1065..1328)
                     /gene="ykgM"
                     /locus_tag="ACHKAU_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5532892.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1692..1793)
                     /gene="ykgR"
                     /locus_tag="ACHKAU_003916"
     CDS             complement(1692..1793)
                     /gene="ykgR"
                     /locus_tag="ACHKAU_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO5532893.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2267..2356)
                     /locus_tag="ACHKAU_003917"
                     /pseudo
     CDS             complement(2267..2356)
                     /locus_tag="ACHKAU_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2908..7161
                     /gene="fdeC"
                     /locus_tag="ACHKAU_003918"
     CDS             2908..7161
                     /gene="fdeC"
                     /locus_tag="ACHKAU_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO5532894.1"
                     /translation="MSHYKTGHKQPRFRYSVLTRCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANRNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KIVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     NADASTGQANLQVDTAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            complement(7282..8139)
                     /locus_tag="ACHKAU_003919"
     CDS             complement(7282..8139)
                     /locus_tag="ACHKAU_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5532895.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            8388..9257
                     /locus_tag="ACHKAU_003920"
     CDS             8388..9257
                     /locus_tag="ACHKAU_003920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5532896.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(9417..10010)
                     /gene="rclC"
                     /locus_tag="ACHKAU_003921"
     CDS             complement(9417..10010)
                     /gene="rclC"
                     /locus_tag="ACHKAU_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO5532897.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(10022..10258)
                     /gene="rclB"
                     /locus_tag="ACHKAU_003922"
     CDS             complement(10022..10258)
                     /gene="rclB"
                     /locus_tag="ACHKAU_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO5532898.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(10367..11692)
                     /gene="rclA"
                     /locus_tag="ACHKAU_003923"
     CDS             complement(10367..11692)
                     /gene="rclA"
                     /locus_tag="ACHKAU_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO5532899.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            11918..12772
                     /gene="rclR"
                     /locus_tag="ACHKAU_003924"
     CDS             11918..12772
                     /gene="rclR"
                     /locus_tag="ACHKAU_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO5532900.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFTREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         3558 a         2773 c         3097 g         3439 t
ORIGIN      
        1 attttcgatt gggcttaaat ttatgttttt ttcccgatga gtgtcaatat gtgaacggaa
       61 aaggaattca atggttaagt caatatttag aaaaactgaa aatattgctt ttttgataag
      121 aagaaaaaga catttggttt cataaggtta ttggcaatat aacgttcaat tcttacttgc
      181 attttatatt ggtcaggaac tttgccctga agtgttagga atagtcttaa tgcgctgtgt
      241 gatggactag taaaaaaaca agttttaagg gtaagggata gaatataaaa ctataatgag
      301 ctgtcattga agatgatttg ccaatcagta agaaatttta atggattaac atgccgcaca
      361 aaatggttat aggtgttcat tacaataagt gatggaaaat tgaacgggcg tatgtacgtc
      421 atctgttcgg taagccccca agagccagat taagtgaagt taaacgaagg ctgccagctg
      481 tcacaggaac gatggcctct ttgtcagggc actgagtgca gttatctgtg cgcgttactc
      541 taactggcac tcgaacgtcg gctacgggtt accggtggat cagactgttc ctgtggcgta
      601 aacgttttta tgttagtacc aacagattta ccacatttat catcttaaca gtttgagtga
      661 aaatgcaggc tgcgccgcaa agggaggaga ctgagtggcg tgttcagtct aaaagagggt
      721 tgatgccagc atacaggggc gaagctggtc aacaagtcaa cattaagata atggaatatt
      781 cggagagaaa cgtcaggcag ttggcctcaa atgaacagga agaatatatc cccaggaaaa
      841 ttaatgttgg tgttataaac acaccaacat taatccgatc tgattattaa tcagacaaat
      901 atcgttaatt cgtcgaattt tgaatcaacg ttttttctta cgaccctgaa cggccttaaa
      961 acgtggatta gatttacaaa tcacatacaa ccgtcctttt cgcttcacaa tctgacagtc
     1021 tggatggcgt tcttttgcgg tacgcagaga gttaagaact ttcatcacgc cccctttttc
     1081 gtgctaacaa aacgaccaaa acgttgggtg aatcgtgcaa catttccttc tgatgtcact
     1141 gttctcagct tccctgtata gaacgggtgc gatttagaag agacatcaat tgtcacgtac
     1201 ggatacgtta cgccatccag ctcaatctca cggtctgttt tgatagtcga gccgatttta
     1261 aagtactcat caacactggt gtcgtggaac accacagtac gatactcagg atggatattg
     1321 ggcttcatca tttttacctg ttatgttata acataaccgt aaagatacat gctcatccgg
     1381 atcatttcaa cacccaatcc ctgtgcccat ttgtggtatg tggaatttcc ccaacgggga
     1441 agtctggcat tgttagcagc cgttggcgac gctcccataa acgcctggat gagtgattga
     1501 tgatggtttt gctgagagta tggatttttc gagaatggag gttatctcca ctttactggc
     1561 cgatgattta tcgcagtatg attgattacc tgacagcgtg tattcctctc ccacggatat
     1621 aataattact tacaaaaagg gagaggatgc atattttaaa tatcactgaa gtgaacagtt
     1681 tattgccgtt attaatagaa atggagaaat aaataggcgt attctacaat tgcgacaaaa
     1741 acaacgatat taatcagttt atgactgatt tgttgtactt tattctcttt cattggtact
     1801 tcctcgcttt aaaaaagagt gcacttcgta agtgccctta tataaataac gagtttggtc
     1861 aaccaatttt ttgacatgta tcacaaattt gaatagatgt attacatcag ctatctttta
     1921 ttgcaccaac gtcattgata tatgtcgact gaagtcagtt ccgggaatga gtctgatctc
     1981 aagactggcc cagtccgggc gttgattggc gctgaggagc atacctcatt tcatcataat
     2041 gtcgtatctc ctggggtgtt atacaagata tcgttgttgg taacctggga aaggaattga
     2101 gttctattaa accgtcaact atgccggata catactggat tacactgcag gcacgcctta
     2161 tgagagaacg tgccgcagtg acgggttaat tatctgaaag aatttgtgag gctgtatcgg
     2221 ttactcattg atttgatagt tttactctcg ggagaataat agatatttaa tccattaacg
     2281 gaaaccagcc agttcctttc gatgcctgaa tttgatccca tagtttatcc ggaatggtta
     2341 attcatgaac aagttgtgtt atcgttcatg agaagcataa cgtaaaggga aaagctcgat
     2401 tagacggcag aatttgtcag gggttatgaa cgaaattcat aaatctgttt gagtgttgcg
     2461 atgggtagtg caagttcgat atctccgcaa tttacagtcc gatgaaggaa aatgaatatc
     2521 cataaaaaat atattggttt atcctggcat atatacctat ttcgacgtat tttcaatagt
     2581 tttaattaaa ggcaggtcat tgttattcac tctgaatagt gaattattca ctgtccgcag
     2641 agtaagaaat ataacttagg tatctattta atgacttgca caaaaagcta aattttcccc
     2701 cataaataaa aatataatcc cgcgcccaac cacctgatga gtggctacag gcactggata
     2761 tattaggtgg cggtgcactt tcttacataa aggtatttcc ttttctgcgg aaaaggaaat
     2821 cgggaaatcc ccggtttttc tgacaagcag acgccattat ttgtgtctgc ctatgttcgt
     2881 taattcgttc atcaggaaat tatctcaatg tcacattata aaacaggtca taaacaacca
     2941 cgatttcgtt attcagttct gacccgctgc gtggcgtggg caaatatctc tgttcaggtt
     3001 ctttttccac tcgctgtcac ctttacccca gtaatggcgg cacgtgcgca gcatgcggtt
     3061 cagccacggt tgagcatggg aaatactacg gtaactgctg ataataacgt ggagaaaaat
     3121 gtcgcgtcgt ttgccgcaaa tgccgggaca tttttaagca gtcagccaga tagcgatgcg
     3181 acacgtaact ttattaccgg aatggccaca gctaaagcta accaggaaat acaggagtgg
     3241 ctcgggaaat atggtactgc gcgcgtcaaa ctgaatgtcg ataaagattt ctcgctgaag
     3301 gattcttcgc tggaaatgct ttatccgatt tatgatacgc caacaaatat gttgttcact
     3361 cagggagcaa tacatcgtac cgacgatcgt actcagtcaa atattggttt tggctggcgt
     3421 catttttcag gaaatgactg gatggcgggg gtgaacacct ttatcgacca tgatttatcc
     3481 cgtagtcata cccgcattgg tgttggtgcg gaatactggc gcgattatct gaaactgagc
     3541 gccaatggtt atatccgggc ttctggctgg aaaaaatcgc cggatgttga ggattatcag
     3601 gaacgcccgg cgaatggctg ggatattcgt gctgagggct atttacccgc ctggccgcag
     3661 cttggcgcaa gcctgatgta tgaacagtat tatggcgatg aagtcgggct gtttggtaaa
     3721 gataagcgcc agaaagaccc gcatgctatt tctgccgagg tgacctatac gccagtgcct
     3781 cttctgacac tgagcgccgg gcataagcag ggcaagagtg gtgagaatga cactcgcttt
     3841 ggcctggaag ttaattatcg gattggcgaa cctctggcga aacaactcga tacagacagc
     3901 attcgcgagc gtcgggtact ggcaggcagc cgctatgacc tggttgagcg taataacaac
     3961 atcgttcttg agtaccgcaa atctgaagtg atccgtattg ctctgcctga acgtattgaa
     4021 ggtaagggtg gtcagacact ttccctgggg cttgtggtca gcaaagcaac tcacggactg
     4081 aaaaatgtgc agtgggaagc gccgtcatta ctggctgagg gtggcaaaat taccggtcag
     4141 ggtagtcagt ggcaagtaac gctcccggct tatcgtccag gcaaagacaa ttattatgcg
     4201 atttctgcgg ttgcctacga taacaaaggc aatgcctcaa aacgcgtgca gacagaggtg
     4261 gtcattaccg gagcaggtat gagcgccgat cgcacggcgt taacgcttga cggtcagagc
     4321 cgtattcaaa tgcttgctaa ccgtaatgag caaagaccgc tggtgctgtc tctgcgcgac
     4381 gccgaggggc agccagtcac gggcatgaaa gatcagatca agactgaact agccttcaaa
     4441 ccggctggaa atattgtgac tcgttcccta aaggccacta aatcacaggc aaagccaaca
     4501 ctgggtgagt tcaccgaaac tgaagcaggg gtgtatcagt ctgtctttac taccggaacg
     4561 cagtcaggtg aggcaacgat tactgttagc gttgatggca tgagcaaaat cgtcactgca
     4621 gaactgcggg ccacgatgat ggatgtggca aactccaccc tgagcgctaa cgagccgtca
     4681 ggtgatgtgg ttgctgatgg tcagcaagcc tatacgttga cgttgactgc ggtggactcc
     4741 gagggtaatc cggtgacggg agaagccagc cgcttgcgat ttgttccgca agacactaat
     4801 ggtgtaaccg ttggtgccat ttcggaaata aaaccaggcg tttacagcgc cacggtttct
     4861 tcaacccgtg ccggaaacgt tgttgtgcgt gctttcagcg agcagtatca gctgggcaca
     4921 ttacaacaaa cgctgaagtt tgttgccggg ccgcttgatg cagcacattc gtccatcaca
     4981 ctgaatcctg ataaaccggt ggttggcggt acagttacgg caatctggac ggcaaaagat
     5041 gctaatgaca accctgtaac tggtctcaat ccggatgcac cgtcattatc gggcgcagct
     5101 gctgtaggtt ctacggcatc aggctggacg gataatggcg acgggacctg gactgcgcag
     5161 atttctctcg gcactacggc gggtgaatta gaggttatgc cgaagctaaa tggacaggat
     5221 gcggcagcga atgcggctaa agtaaccgtg gtggctgatg cgttatcttc aggccagtcg
     5281 acagtgtctg tagccgcaga tcgcgtaaaa gcaggcgaaa gcacaaccgt gacgcttatt
     5341 gcaaaagatg cacatggcaa cgctatcagt ggtctttccc tgtcggcaag cctgacgggt
     5401 gctgcgtctg aaggggcgac tgtttctggt tggaccgaaa aaggtgatgg ttcctatgtc
     5461 gctacgctga caacaggtgg aaagacgggt gagcttctcg tcatgccgct attcaacggc
     5521 cagccagcag ccaccgaagc cgcgcagttg actgtcattg cgggggagat gtcatcagcg
     5581 aactctacgc ttgttgctga caataaggct ccgaccgtca aaacgacgac gaaactcacc
     5641 ttcaccgtga aggatgcgta cgggaacctt gtcaccgggc tgaagccaga tgcaccgcag
     5701 tttagtggtg ccgccagcac ggggacagag cgaccttcaa caggagactg gacagaaaca
     5761 agtaatgggg tctacgtggc gaccttaacg ctgggatctg ccgcgggcca gttgtctgtg
     5821 atgccgcgag tgaacggcca aaatgccgtt gctcagccac tggtgctgaa tgttgctggt
     5881 gacgcatcta aggctgagat tcgtgatatg acggtgaagg ttgataacca gctggctaat
     5941 ggacaatcga ctaaccaggt aaccctgacc gttgtggaca cctatggtaa cccgttgcag
     6001 ggacaaaatg tgacgctgac tctgccgaaa ggtgtgacca gcaagacggg gaatacggta
     6061 acaaccgatg cggcaggtaa agccgacatt gagctgatgt caacggttgc cggggaacac
     6121 agcatcacgg cctcagtgaa taatgctcag aagacggtca cggtgaaatt caacgcggat
     6181 gccagcaccg gtcaggcaaa cctgcaggta gacaccgctg ctcaaaaagt ggcaaacggc
     6241 aaagatgcct ttacgctgac ggcgaacgtt gaggataaaa atggtaaccc tgttccaggg
     6301 agcctggtga cctttaatct gccccggggt gtcaagccgc ttacaggcga taatgtctgg
     6361 gtgaaagcca acgatgaggg gaaagccgag ttgcaggtgg tttcagtgac tgccggaacg
     6421 tatgagatca cggcatcggc agggaatagc cagccttcga atacgcagac tataacgttt
     6481 gtagccgata aggctaccgc aaccgtctcc ggtattgagg tgatgggcaa ctatgcgctg
     6541 gcggacggca aagccaaaca aacgtataaa gttacggtga ctgatgccaa taacaatttg
     6601 gtgaaagata gcgaagtgac gctgactgcc agcccggctt cgttaaacct ggaaccgaat
     6661 ggcactgcga caacgaatga gcaagggcag gctattttca ccgctaccac tactgttgcg
     6721 gcgacataca cactcaaggc gcaagtgagt cagaccaacg gtcaggtatc aacgaaaact
     6781 gccgaatcta aattcgttgc ggatgataaa aacgcggtac tcaccgcatc atctgatatg
     6841 caatctctgg tggcggatgg gaaatcgact gcgaagctgg aggtgacact gatgtcggca
     6901 aacaaccccg ttggcgggaa tatgtgggtc gacattcaga cgccggaagg ggtgacggaa
     6961 aaggattatc agttcctgcc gtcgaaaaat gaccatttcg tgagcggaaa aatcacgcgt
     7021 aaatttagta ccagcaagcc tggtgtctat acgttcacat ttaacgccct gacctatggc
     7081 gggtacgaaa tgaagccagt gacggtgacc attaccgcgg tggatgccga tacggcaaag
     7141 gacgaggagg cgatgaaata attttaagaa tatgaaagta acattctaat aaacaataat
     7201 ggcgttgaca tcttcaacgc cattatttat tgaatttcct gacagtagtc tcgttataaa
     7261 ttcatttaga tatagcgaaa actaaaccgg acgaacgggg ataaaaattt cgcaaacaat
     7321 gttattgcta tcaatttcga cgaaattttc attgtaataa aaccgctcaa tatcactgcc
     7381 gttcactctg gctaaccccc tggcgggtaa ttccatcaga tagaggtttc gcgcccattc
     7441 agggtagtgc tcccactcac cttcatagcg gaaagaggca tacagtcctc ggttaattgt
     7501 ccactctttc ggcatctctg aatcggtttg tttgcatacc gtaccggcaa aagtttcaac
     7561 cgtgagcaaa tcgaccgatt ttgttgacgg ttcaaacgtg gagacgaaat agatatccgt
     7621 cttaaaatta ttcatacatc tggctaaatg atgacgtcgc agtttgactt cttcatccgg
     7681 tgatttattc gacacttcaa tatcatattt aaatgaattg ccaaaaatag gtgtctcagg
     7741 aaagttgact aatctacact cggtcttttg ctgttgacgt attaaaaaag aagggaagat
     7801 atttgcgaga tcccaataat cacggtggcg gtatacacgg ggagagcagc cgaataactt
     7861 tttaaattcg cggctgaatg actgctgtga atcaaaatgc aaagagagtg caatatcaag
     7921 catagatttc gcggtgagcc ggacaagaat ggcggcacga caaagccttc gtttgcgaat
     7981 gtattctccc aaaggcacat gcatgaaatt tcggaacaga agctggatgt tgcgtctgct
     8041 gtagccagat ttctgtgcga tatcttcgat tgaaataggg tgctcaagat tgcactcaat
     8101 ccactccagg agctgctgta gaatcttctg cctgatcatc acattctcca ttgattcttc
     8161 gtcatatcgc acattttcgt tatgccctgg gccctaagtg tatcaaaaac agcaagggcg
     8221 aaggcaaaag cactggtagc gtctgcttcg ttgtccccga aaatagagag gtttcagtcc
     8281 tgcattatta atgaattttt tgcataagtg atatcaaaat ccacgtacta atttgaggtt
     8341 acgttttaac gtagactcat tgttcatgcc taatggaggg actgacagtg gaattttccg
     8401 tattaagtaa caatctgaaa atgccgatga tgggatttgg tgtttttcag gttaccgata
     8461 aaaacgtgtg caaacagtca gtgttgaacg ctatccgcac aggttatcga ctcatcgaca
     8521 ctgcagcggt atacggtaat gaagatgccg tcggtgaagc cgttcgtgaa gctatatctg
     8581 aaggtttatg tacccgagat gagttattta taacgtctaa attgtgggtg caggatatgt
     8641 tgaatcagga tacggcagca gcaggtattg aagcatcatt aaaaaaatcg ggactagagt
     8701 acttcgacct ctatttattg caccaggcta tgcgcgatta tttcagtgcg tggcgtgcac
     8761 tcgaagatgc ctatgaagag ggcaaattaa aagcaattgg ggtttccaat ttctatcctc
     8821 atgttctggc gaacttttgt gaaacggtaa gagttaaacc gatggtcaac caggtcgagt
     8881 tgcatcccta ttttgcccaa ccagaggcgc tggcaaccat gaagtattat aacgtgcagc
     8941 ctgaagcatg ggctccgtta ggtggtggac gacataaacc ctttgaaaat aatctgcttc
     9001 agagtattgc agatgcccat caaaaatcga tttctcaagt cattctgcgt tggaatattc
     9061 aacggggagt ggtcgttatt ccgaaatcga cacatcaaca gcgtatcgaa gaaaattttg
     9121 ctatctggga tttctcactg acagagaaag aaatggcaca aattagttcg cttgatttgg
     9181 gttatgttgg ggagtcggta aaacatttta atcctgaatt tgttcgtggt tgtcttgctg
     9241 taaaaataca tgattgatat taatcatata ttttacctga gacgacaaga atcttttaac
     9301 aggggagtga tattgatctt cactctgtca tatctccggt aatatggcgt caggcttcat
     9361 catcagaaag ggggcctgac gcctgaaaaa gtgaacaaca gacagtgttc ggattatcaa
     9421 tattcagttt ttaacgttga actggattca ttactgcgtt gtttaagaat ttcccgcgcc
     9481 gaatctgcca ttatcatgac agcacctgcc agcatcagag tatctttcaa taccaggcga
     9541 ccagcaccgg ataaataagg gaaaccatga tgagcgtcac ccaatgcggg tacccatgcc
     9601 tccggggtgg tgattaaaaa tgagagtgtt accagcggcg tggtaaatgc catcagtcct
     9661 cccaataaac ctaaccagcg attgacagga ttagccaaaa ccagcagagc aataatcacc
     9721 tccacgacgc caagaccgtt ggaaaaacca taggtattat tggccgtttg ccatgccctt
     9781 gcttctggtt tgtattcgcc ttcgtgagtc agatactgtt tatagtcttc cgggtgttca
     9841 taaaagaacg acattagtgg actgtttgcg acgaatggtg taatgctgtc tgcctcgtaa
     9901 gggacaaact ttaataaccc aatccacata aaaacaattg caatactcag acgaatcaat
     9961 gtcaggccaa ttttatctcc ccgacttaac aggtgcaggt atttttccat tgtgaacatc
    10021 cttatttata caatactgct gttgcggttg aattattatc aattcgcatc gatgtgactt
    10081 tccaggatga agcattatat tttctggcta tatcagcaat ggtatcctcc agaactgtcg
    10141 gtgatgctga tactgacgag acgctgatat gtttcagaat tattttatca tctgcattgg
    10201 tggaaaatgc cataacgcca gataatagtg ttgcaaataa aacagatttt ttaaacatat
    10261 aaaataccat caatgaagtg attaagaagt tatagagtaa cagggaatta ataagattct
    10321 tttctctgag acgccagaat atttgttctg gcgtctgatt ttgagtttat ttgactaatg
    10381 aaaatagatc attgagtgat tcgctcatcg acggatgagt aaatatctga tcgcgtaata
    10441 tgctataagg cagcccggca tccatcacca ttttcactat attgatcatc tcgtgggagt
    10501 caacacacag cagtgatgcc cctaatatac gttgggtttt attatcaaca atcgctttta
    10561 ataccccacg agtatcattc atcactctgg cacgcggaat tgcagctaca ggcaatgtca
    10621 ccacctgaat atcagcacca ctctctctgg cctgttcttc tgtcatacca accctggaca
    10681 gtggcggtgt catgaatacg gaataaggca catttttccg atcatcagta ctacgtctgc
    10741 cttctcccag caactcatca cgcacaatgc ggtaatcatc cagtgaaata taagtaaatt
    10801 gcagcccgcc ggtaacatct cccatcgccc aaatattgtc tgcggtggta tgtaattgct
    10861 tgtcaacgac aattgccccg cgctcgttta ctgcgatacc ggcattttct ggatgtaacg
    10921 aagcggtagc cggttgacga ccggaagcta ttaacagtgc atccaccgcc agttgggcgt
    10981 gctcgctatg cacttgcact tgattttcat ggtgactgat tcgctccaca tgggcattga
    11041 ggataatatc gacgccctga tcgcgtaaaa tcgtcgcgat attatcagca atatcccgat
    11101 cttcccgagg caaaaacagc gaagctgctt ctaaaatggt taccttgctg ccaaaattag
    11161 cgaacataga ggcgaactca acgccaatat atccgccgcc caaaatacct aaatgcccag
    11221 gcaattcttt tagattaagt aatccggtgc tgtcatatac tcctggcgtg gtggtaattc
    11281 caggaattgg cggaaccacg gtttgtgcac cggtattaat aaaaattttc tcgccatgaa
    11341 tttccagatt tgcctcaggc cgatgaacac gcaggctatg attattgata aactccgcct
    11401 ggccgtcgat cacgtcgata ttgggcatat ccgcaagatt atgaaaattc ttattacgta
    11461 aaaaattaac cacttcattt ttacgctgta tggcacggac aaaatctgtg tgctgctgtg
    11521 cgtcatgaac caatgttttg gttgggatgc agccgatatt aatacaggtc ccgccataca
    11581 ttgcatttga ttgttcgatg agagccactc gccaacctgc ttttgccagc gtgacggcta
    11641 atgtttttcc agccttgcca aaaccaataa tcactgcctg atatttattc atgatgattt
    11701 cctgtcgaaa cgcgttcatt tgacatcatg actatagacg caaaagagca aataccattt
    11761 cttcacaacg cttgatattt tgtcttatcg tctggtattt tctttttctt aatcagaatc
    11821 acaaaacaaa aatcatgatt aaccatttga tggtattaga ttagttattt actaagattg
    11881 ttggtgtttg taatcaaaaa ccactcagga gtctgatatg gatgccctta gccgtttgtt
    11941 gatgcttaac gctccacaag gaacgatcga taagaattgc gtgttaggaa gtgactggca
    12001 gcttccacat ggtgccgggg aattatcggt tattcgttgg catgcgttaa cgcaaggagc
    12061 ggcgaagctg gaaatgccga cgggagagat ttttacatta cgcccgggaa atgtggtcct
    12121 gctaccacaa aattcagctc atcgcctgag tcatgtcgat aatgaatcga cctgtattgt
    12181 ctgcggcact cttcggttgc aacattcggc gcgttatttt ttaacgtctt tgccggaaac
    12241 gctgttttta gcaccggtta accacagcgt ggaatataac tggctgaggg aggcgatccc
    12301 gtttttacaa caggaatcca gatcggcgat gccgggagtg gatgcactgt gtagccagat
    12361 ctgcgctaca ttctttaccc tcgcggtgcg tgagtggatt gcacaggtta atactgagaa
    12421 aaacattctc agtttgcttc tgcatccacg tcttggtgcg gtaatacagc aaatgctgga
    12481 aatgccagga cacgcctgga ccgtcgaatc gctggccagc atcgctcaca tgtcccgggc
    12541 aagttttgcc cagcttttcc gtgatgtttc cggaaccacg ccgctggctg tattaacaaa
    12601 gttgcgtcta caaatagcgg cccagatgtt tacccgggaa atgctccctg ttgtggtgat
    12661 cgctgagtca gtaggctatg ccagtgaatc atcttttcac aaggcgtttg tccgcgagtt
    12721 tggttgtact ccgggagaat atcgggaaag ggtcagacag cttgcaccct gaataaaacc
    12781 gccagaaatc agggcaaaga taatccgcat tccgggagtt gtgagccttt caactatttc
    12841 tatttccaac ggtggtttgg gctttat
//
LOCUS       DAZFJF010000122        12662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFJF010000122 DAZFJF010000000
VERSION     DAZFJF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.122"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..227
                     /locus_tag="ACHKAU_003925"
     CDS             3..227
                     /locus_tag="ACHKAU_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532901.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            479..685
                     /locus_tag="ACHKAU_003926"
     CDS             479..685
                     /locus_tag="ACHKAU_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532902.1"
                     /translation="MAEEKKGGVSVYISPDIVKALKERHQQNVKAGIAAGLDPLAMVE
                     PSTGWQVRAYLRAALGMNQTHGGE"
     gene            685..1740
                     /locus_tag="ACHKAU_003927"
     CDS             685..1740
                     /locus_tag="ACHKAU_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5532903.1"
                     /translation="MAGKATALNTNQLFAYLNRGDIAEFKFSPLFTTLFFPNVATFNT
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNLIEGEGIERYE
                     IDWKIPESNIIEQADGKKWSELDKETHDPIYDIELYADQGNCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDIALVVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            1753..2088
                     /locus_tag="ACHKAU_003928"
     CDS             1753..2088
                     /locus_tag="ACHKAU_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5532904.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLAENGEFK
                     PWDGQESGKAVYLTSYPVDTSTQKSAQCYKTGIFNIAAVNWPESADTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            2101..2514
                     /locus_tag="ACHKAU_003929"
     CDS             2101..2514
                     /locus_tag="ACHKAU_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFO5532905.1"
                     /translation="MATNESIMTLPLASKFKAEARAMADRGLSTYEAVYQLNSLEEKD
                     KPRADAIMALHESDDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            2720..3262
                     /locus_tag="ACHKAU_003930"
     CDS             2720..3262
                     /locus_tag="ACHKAU_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5532906.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDILPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            3518..3799
                     /locus_tag="ACHKAU_003931"
     CDS             3518..3799
                     /locus_tag="ACHKAU_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532907.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(4401..5861)
                     /gene="phoQ"
                     /locus_tag="ACHKAU_003932"
     CDS             complement(4401..5861)
                     /gene="phoQ"
                     /locus_tag="ACHKAU_003932"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO5532908.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(5861..6532)
                     /gene="phoP"
                     /locus_tag="ACHKAU_003933"
     CDS             complement(5861..6532)
                     /gene="phoP"
                     /locus_tag="ACHKAU_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO5532909.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(6700..8070)
                     /gene="purB"
                     /locus_tag="ACHKAU_003934"
     CDS             complement(6700..8070)
                     /gene="purB"
                     /locus_tag="ACHKAU_003934"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO5532910.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(8074..8715)
                     /gene="hflD"
                     /locus_tag="ACHKAU_003935"
     CDS             complement(8074..8715)
                     /gene="hflD"
                     /locus_tag="ACHKAU_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO5532911.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(8751..9857)
                     /gene="mnmA"
                     /locus_tag="ACHKAU_003936"
                     /gene_synonym="trmU"
     CDS             complement(8751..9857)
                     /gene="mnmA"
                     /locus_tag="ACHKAU_003936"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO5532912.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(9911..10372)
                     /gene="nudJ"
                     /locus_tag="ACHKAU_003937"
     CDS             complement(9911..10372)
                     /gene="nudJ"
                     /locus_tag="ACHKAU_003937"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO5532913.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(10382..11035)
                     /gene="rluE"
                     /locus_tag="ACHKAU_003938"
     CDS             complement(10382..11035)
                     /gene="rluE"
                     /locus_tag="ACHKAU_003938"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO5532914.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            11207..12457
                     /gene="icd"
                     /locus_tag="ACHKAU_003939"
     CDS             11207..12457
                     /gene="icd"
                     /locus_tag="ACHKAU_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO5532915.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(12560..>12662)
                     /locus_tag="ACHKAU_003940"
     CDS             complement(12560..>12662)
                     /locus_tag="ACHKAU_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO5532916.1"
                     /translation="LKSSQECPPGESKITNKCLHNSCIIKICPYGLK"
BASE COUNT         3278 a         3099 c         3214 g         3071 t
ORIGIN      
        1 caatgccgat gaaatttgat gagatattaa aacagcgtga taaataccat gctgacaaca
       61 tggagacgat gagcatcaat gattaccgcg cattcctgga gacgggcgca ctgattgaaa
      121 aggatcagca tggttttgtg agatgtgctc tatccggtga aatgctggcg gtaaatcctg
      181 aacagataga tgcattgata gaatttctga aagagatcag agactgagcc agcacacagc
      241 acacatagcc ggagcaatcc ggcttttttg cgcccaaaaa aagcccgata aggtcagagg
      301 gttcttatcg ggcttttgca tatgaggttt ttggatgcac tggcgttcgt gatcgggata
      361 atcatttcat aatttgcaac ataactcaat atcattgcat aaaatgcaat tctgattata
      421 atcaggactg gataaacatc cagttatgat tttttaagtc gaagaggaat ttcttactat
      481 ggctgaagag aaaaaaggcg gtgtttcggt gtacataagc cccgacatcg tgaaggcgct
      541 caaggaacgc caccagcaga acgtaaaagc aggcattgcg gcaggacttg atccgctggc
      601 gatggttgag ccgtcaacag gctggcaggt ccgcgcctat ttacgcgcgg cgctgggcat
      661 gaatcagacg catggaggtg aataatggca ggcaaagcaa cggcacttaa cactaaccag
      721 cttttcgcgt acctgaatcg cggggatatt gcagaattta aattcagtcc gctgtttacc
      781 acgctgtttt tcccgaacgt ggcgaccttc aacacccaga acatcatgct ggacaccctg
      841 gacattgaag aggttacgat gtcagcgttc tgttcgccta tggtgggtag tcaggtccag
      901 cgcgataaag ggtacgaaac cagcacgatt aaaccaggct acatgaaacc caaacacgaa
      961 atcgatccaa caaaaaccat catgcgcatg gctggagaag atccggcaca gcttaacgat
     1021 cctacctatc gccgtatgcg cctgattact ggcaacatgc gccgccagat aaacgccatt
     1081 aaggcacgcg tggaatggct ggcggtgaat gcggtaacga ccggaaaaaa cctcattgag
     1141 ggcgaaggca tagaacgcta tgaaatcgac tggaagatcc cggaaagcaa tatcatagaa
     1201 caggctgacg gtaaaaaatg gtctgagctg gataaagaaa cacacgatcc aatctatgac
     1261 atcgagcttt atgctgatca gggcaattgc cccgcaaacg tcatgattat gggcgctgaa
     1321 gtatggcgca cgttacgcag ctttaaaaaa ttccgcgaac tgtacgacct ttcccgtggt
     1381 tcagaatccg ccgcagaact ggcctgtaaa aacctgggtg aagtggtgag ctttaaaggc
     1441 tatctgggcg atattgccct tgtcgtctat tccggcaaat acactgacag cgacggcacc
     1501 gaaaaatatt tccttgagcc tgatttgctg gtcctgggta acaccaacaa taaagggctg
     1561 gtggcctatg gtgcgattat ggatcaggaa gcggtcagaa cgggcgcaac gcaaaacatg
     1621 ttttacccga aaaactggat tgaggacggc gatccggcga ttgagtacgt gcagacgcac
     1681 agcgcaccgc agccggtacc ggcagatatt cgcaaatttg ttaccgtcaa aattggttaa
     1741 cggggggatt ctatggacac tccatacatt gagttatttg caggtagtca gcaggtatcc
     1801 acgacgctgg tacattttgc tgctgatgct ggcgttattc aggaatttac cccgctgatg
     1861 ctggcggaaa atggcgagtt taagccgtgg gatggtcagg aatctggcaa ggctgtttat
     1921 ctgacctcgt accccgtgga cacgtcgacg cagaaatcag cacagtgcta caagacgggg
     1981 atattcaata ttgccgccgt taactggcct gagagcgccg acaccgacgc gaaaaaatgc
     2041 gccgcctttg cgggttctgg cgtatccgtt cagccgctgg cgcgataagc agggggaacg
     2101 atggcaacga atgaaagtat catgacgcta ccgctggcga gtaaatttaa agccgaagcg
     2161 cgggcaatgg ctgacagagg tttatcaacc tacgaggccg tatatcaact taacagcctg
     2221 gaagagaagg acaagccgcg cgctgatgcg attatggcgc ttcatgaatc tgacgactat
     2281 cagccgctgt tacgtgcaat ggcaaacgtg ccttgtattg atgttgatac ggctaaaaac
     2341 atcctgaaca tgaccataga gcaggaacgc ccgaaggttg caccagagct taccgcagcc
     2401 tttgaaaact ttatggacat gcacagccca caagccgtat cagctggcat ggcatacgat
     2461 ggcagaaacc cgggcgatga cggcgacatc gatcgcatac tgaaaaccat ctgagacaag
     2521 gccggagaaa tccggctttt ttgcgggtcc tttccggcat atggacctgt tacggggcgg
     2581 agacctcgcg gattttcgct atttatgagc cttttcaggg gggtggtggt ggttttgttg
     2641 tttgatctat ctttatgaat gaaaagggaa agatgcaagc aatacaccaa cctgaagcag
     2701 taattaagtt ggtgtattaa tgaaatctca tctgatgaac aaaaaaaaca tggcgaaaag
     2761 ctgccgtgta agtgcgacag cgttcgacaa gtggggagta actcccgttg agcgtaaagg
     2821 ccgtgaggcg ttttatgatg ttgccagcgt gatagaaaat agggttaaca atgcaattag
     2881 ccagcttata aacgacaaag gcgagattga cgatgatgaa cttttacgag tcaggatcag
     2941 attactgaca gcacaggcag aggcgcagga acttaaaaac gatcgcgatc gcggtgacgt
     3001 gattgatact gagttttgcc tgtacgcgct ttcaaagctg gcgagtcaga tttcatcaat
     3061 catggacatc ctcccgctta ctatgcaaag gagcttccca cagattaccc ccgccatgct
     3121 ggacaggctt aaaagggaag tggttaaagc ctgtaatgcc agtgccagag ttgccgacaa
     3181 cctcccacag atactggctg attacttgaa agaaacaacc ggaaacgtac cggaaaagtt
     3241 gctacagaag aaaggcgagt aacagacgcg caattattga acaaaaatga gaaaggatct
     3301 gataatggca ttttctggct aaaatgcctc gtgtcagatc ctttcttttt ttaatatttt
     3361 cattgttaaa cagtctgtta cagagaagaa gatcggatct gacttttgcc ggaaaattct
     3421 cataaacaga taaaaaccgc gaggtcgccg ccccgtaacg ggccataatt ccaggaagga
     3481 cccgacgaca ccagactatc agagcgatgg gggcacaatg acagaagccg aactactggg
     3541 attaatccgc cgcgtcgccg gaatcagcca gcagcatgac gaacaggcca cacagccgga
     3601 cagcgtgacc gctgaaaatt atgtgcgtgt tgtagcggaa gtgatgcgcc gtgatggtat
     3661 agaacttaat ggcgttgata tgcgcaacat acggaaccgc gttcttgaaa tgctggccta
     3721 caatcgccgc gttgaactgt atcgggagaa agaaaaaata acgtaccact ggaagaagcc
     3781 ggaacggctg cggcggtaac ttgctgatat tcccgataac gcaaaattgc gctggctaca
     3841 tccctcacat ctgaggactg ttaaccagaa ggccggaata tattcacaaa ctacgaaatt
     3901 ttcgtagttt aattaatggt cagattaatc gctaacccgc tgatattttc ggaaacctca
     3961 atttgaggaa gtcggaagcg tcacccttca tattctgaat ggtcataatg accacgcaga
     4021 ccatcaaggc aaccacaacg aacatcgtta cggttggagc ttttacccca ctgggggaaa
     4081 ggtattacga taatcataac acctaccgaa caggtaaaac ccaccagcct gattaacagt
     4141 taaccggaaa aaaaatccgg tagccccgtt atggggatcc ccatatcgac attaacgccc
     4201 ctcatgaatt ggtgcgcttc cccctggaaa gattacccgc ctgtatattt cttgtgtcta
     4261 tttgttccac gttgtttcat acagtgcacc gaacggtgta gttaatggtg tagtcaattt
     4321 gctgttttta gcactttttg cagtgtgtgt tgtttaatta aatcaatggg ttgccttgct
     4381 gtggtgataa aaggacatat ttattcatct ttcggcgcag aatgctggcg accaaaaatc
     4441 acctccatcc gcgcaccgcc cagcatgctc tctccggcga cgattttacc ctcatattgc
     4501 tcggtgattt cgcgggctac cgccagccct acaccttgcc cagggcgtaa agtatcaacc
     4561 cgttgaccac ggtcgaaaat gacctctcgc ttgcttaatg gaataccggg gccatcatcc
     4621 tcgaccacaa tatagagatg ctcgtcggtt tgccttgcag aaatttcgac aaactcgagg
     4681 caatatttac aggcattatc cagcacgttg cccatcacct cgacaaaatc gttctgctca
     4741 ccgacaaagc tgatctctgg cgaaatatcg agagagatat tgaccccttt gcgttgatac
     4801 actttgttca gcgctgaggt gagattgtcc agcagtgggg cgaccggatg cagctcgcgg
     4861 ctgagcaatg tcccgccgcg catactggca cgatgcaggt agtagccaat ttgctgtgaa
     4921 atgcggctga tttgctccag cattaccggc tcagcatcac tgacgctcat cttttcacta
     4981 cgcagagaac gcagcgtact ttgcagcacc gccagtggcg ttttcagact atgggtcagg
     5041 tcggtgagcg tcgtacggta tttgtcgtaa cgttcgcgtt cactttttaa caatcggttc
     5101 aggtttcgta ccagactggt cagttctcgc gttgtggctg gattgagcaa ttcgcggtta
     5161 tgttcttcca gttcgcggac ttcttttgcc agggcttcga tggggcgtaa actccaccag
     5221 gcggcgaccc acagcagcgg gatcactaac agcagattgg ctgagagcac atagataaac
     5281 cagctccaga ccatatagga actttttagc tccaccggaa tggtatccac caccacaatg
     5341 gttaattttg gcatccgcga tgttgccggg tagacgttta ctgccaccga gtgggtcatc
     5401 tccgcgtcgt catcatcttc ccgcacttcc tgcaactgtt gctgtatcga atgatctcca
     5461 ctcagcaaga ggctggtatc gttaacatcc gcttcaattt catgaaaacc attcgatttc
     5521 agccagtcag gctggatcat cttcatcagc cagggcacgt cacgttgcgc ccataaaagc
     5581 tgcccgttct catcataaat tagcgtcatg gtggggcttt gcttgtcgat attttcgggt
     5641 aactcgacat gcaacttatt gttttcccac ttcgcaaggg tatagaacag attgctctcg
     5701 ccacgtaaca gccgaaacgt agttttatcg aaactgacgc tataaccgat cagcgcgacc
     5761 attccgtagg caagcgaaag caccagtact accgcagccg ttgccaacag aaaacgtacc
     5821 cgcagtgaga gcgggaaaaa aagacgcagt aattttttca tcagcgcaat tcgaacaaat
     5881 agccctggcc gcgaacggta gtaatcactt cttgcggata ttgtgcctga atttttttgc
     5941 gcagacgtcc catcagtaca tcaatggtat ggctttcccg cagctccgca tccggataga
     6001 gttggagcat taacgaatct ttgctcacca ctttgccatt attgcgtatc aacgtttcca
     6061 taatggtgta ttcgaacgcg gtcagtttga tcacttcgtc attaatagat aattcacggc
     6121 gagagagatc aacctgaaac gggggtagcg aaatgacctg tgaagccagg ccgctattac
     6181 gccgcattaa tgcctgcatt cgcgccatca cctcttcaat atgaaacggt ttagtcacgt
     6241 aatcatcagc accggcactt aatacttcga ctttgtcctg ccagctttca cgggcggtta
     6301 ataccagaat cggcagtgaa acatcattgc tacgccagcg gcgaatcagt gacagaccgt
     6361 cctcgtcagg caatccgaga tcgacaatcg caatatccgg taaatgttca ttgagataat
     6421 aatcggcttc tttggcatct tctgcgtcat cgacctgatg accagcatcc tgaatctgaa
     6481 ctttaaggtg gtgacgtaac aacgcattgt cttcaacaac cagtacgcgc atttttattt
     6541 ctccctgtct taattattaa aatagtgtaa cgcgattatg tgtttatggg ggtaaacatt
     6601 aaataaacca gcggggaggg gaggtaaagt gaaaaaagaa aaagtggata attttaataa
     6661 acaggccgga cagcatcgcc atccggcact gatacgagtt tatttcagct catcaaccat
     6721 ggtgatagct cgaccaatat agttagccgg cgtcatcgct ttcaggcggg ctttctcttc
     6781 ttctggcaac gccagaccat cgataaactg cttcatgcct tcggcgtcaa cgcgcttacc
     6841 gcgagtcagc tctttcagct tctcgtacgg tttttcgatg ccatagcgac gcataactgt
     6901 ctggattggc tcagccagca cttcccagtt gtgatccagc tcatccagca gatggtcacg
     6961 gtttacttcc agtttgctca cgcctttcag ggtggattga tacgcaatca gcgcataacc
     7021 gatacccacg ccgaggttac gcagcacggt ggagtcggtc aggtcacgct gccagcggga
     7081 aaccggcagt ttgcttgcca gatgctgcaa taccgcgttg gaaaggccca gattcccttc
     7141 ggagttttcg aagtcaatcg ggttaacttt atgcggcatg gtggaagaac caatctcacc
     7201 agcaatggtt ttctgtttga agtggttaag ggcgatataa ccccagacgt cacggtcaaa
     7261 gtcgatcaga atggtgttga agcgcgcaac gcaatcaaac aattcggcaa tgtagtcgtg
     7321 cggttcgatc tgggtggtgt acgggttcca ctgaataccc agcgaggtga cgaactcttc
     7381 gctgaactga tgccagtcaa cttccgggta agcggcgata tgggcgttat agttaccgac
     7441 cgcgccgttg attttgccga ggatctccac ctgattaagc tggcggtact ggcgctccat
     7501 acggtaggcg acgtttgcca tctctttacc gatggttgac ggcgtggctg gctgaccgtg
     7561 ggtacgagac agcagcggga tatcgcggta ctgaacggcg agatctttaa tgccatcaat
     7621 cagttgacgc cagtacggca ggatcacttc atcacgcgcg gttttcagca ttaatgcgtg
     7681 ggagaggtta ttgatatctt ccgaagtaca ggcaaagtgg atgaattcag aaaccgcgtg
     7741 cagttccggg atctccgcca ctttttcttt caggaaatac tcaaccgctt taacgtcgtg
     7801 gttagtggta cgctcgatgg ttttgatgcg tgcggcatct tcttcgctga aattggcgac
     7861 aattgcatca aggtaaccga ttgcgtcggc agcaaaagca ggaacttcct tgatcgctgc
     7921 gtgcgcggcc agtttttgca gccaacgtac ttcaacttgt acacggaatt tcagcaaacc
     7981 atattcgctg aaaatcccgc gcagcgcgct gactttatcg ccgtagcgtc catcgacagg
     8041 ggaaacggcg gtcagtgagg ataattccat agatcacaac tccggggtta aatgagcaag
     8101 aatttgtttt gcctgagtgg tcaggcgatt acgagaaaac atcagttgca gacgtccgcc
     8161 gccgacctgg tgccagagca cggcggcgcg aatgcctgcc agcagggttg cgcgaacttt
     8221 cgcctgcact tgtgggcttt gcagtacagc aggggaaccg gtgacctgaa tgcgcgggcc
     8281 aagcgggctg atcacatcaa catagatagc agccatcgcg ctcatcagcg tttcggactg
     8341 taaatcgaag tgttcgagct ggcgttgcag gccgttgatc cggttgccca gagtgtcgag
     8401 cgcgcctttc gctgaggaga gtttgcgctc aagcaccatc aagctgagtg tgtagcgggt
     8461 taattcggcg tttaagccct ggcgactgct ggcattgagc acgccgagca gggtttccag
     8521 cccgacgcgc aggttggctt cgctaccgcc aaaaaccgcc agcgtcgagc tggggttcat
     8581 atcaataata ctgttgagtg agacgtgtag cgcatcggca tcacaatgcc cctggtgagc
     8641 gagttgttgc accaggcgtg ccgactgaca aataccggcc agggcgaggg tgatgtcata
     8701 gtaattcttt gccacgttca ctgcttcctt gttttaagta aagataataa tcagaccggc
     8761 agcggcagac gctgctcaat aataccgcca ccgaggcaca cttcaccgtt atagaagacg
     8821 gcagactggc ccggcgtcac ggcggcaacc ggttcatcga aaatcacttc aatgcgatca
     8881 tcgtccagcg ccttgacggt gcaagggata tcggtctggc gatagcgggt ttttaccgtg
     8941 caacgcatag tgccggtgaa tggttcgcga tcgacccagt gcaactgctg ggcaatcaac
     9001 ccgacagaca tcagccgcgg gtgttcatgg ccctgagcga caaccagaat gttgttttcg
     9061 acgtctttgt ccaccacata ccacggttct tcggtacctt ctttggtgcc accgataccc
     9121 agacctttac gctgaccgag agtgtgatac atcagcccct ggtgctcacc aatttcatcg
     9181 ccatcgacgg taatgatttt gcccggttgc gccgggagat aacggcccag gaactcgcgg
     9241 aatttacgtt cgccaatgaa gcaaatgccg gtagagtctt ttttcttcgc ggtgaccaga
     9301 ccaagatctt cagcaatctt acgcacctgc ggtttttcca gttcgccgac cgggaacagg
     9361 ctttgcgcaa tctgctcatg gctgagcgta taaaggaagt agctctggtc tttattgctg
     9421 tccagaccac gcagcaggcg gctcttgcca tcgacatcgg cccgacgtac gtaatgaccg
     9481 gtagcgatat aatcggcacc taaatcttcg gcggcaaatt cgaggaaggc tttaaatttg
     9541 atctctttgt tgcacagaat atccggattc ggcgtgcgac cggctttata ttcggcaagg
     9601 aacagttcga agacgttgtc ccagtactcg gcagcaaagt taacggtgtg cagttcaatg
     9661 ccgagcttgt cgcagacagc ctgggcatca gccagatccg ctgccgctgt gcaatattcc
     9721 tcaccgtcgt cttcttccca gttcttcata aacaggcctt cgacctgata tccctgttgt
     9781 tgcaacagcc aggcagaaac ggaggaatcg acaccgccgg acatgccgac gattactttt
     9841 tttgcggttt cagacattgg atcactcacg acattgaact tcaaggcggc gtattctatc
     9901 acgcagcctt ttagatgaca ccctttgtaa aaggccagtt aaaatcgcca atcatctcca
     9961 gcggatagcg ttgcccgctt tgataacaac ggatactttc cgccaccagc ggcgaacgaa
    10021 gatttgacgc ctgtaaaatt tcttcggcgc tgacccaacg acagcagtcg atatcgctgt
    10081 catgaggctg ggtcgggcat atttgctcaa gctcaatggc aaagaggaaa cgcaaaaacg
    10141 gcgttttatc tggcgcaatc cactgatgca tacgaataaa gtgttgcggc tgcgcgctga
    10201 tgccggtttc ttcccacagt tcacgggcgg cggcttccac taaggtttca tcggcttcca
    10261 gatgcccggc aggttggttc cataacgctt taccattaat cgtctcttca acgactaaaa
    10321 atttgccttc tgcgtgcacc acgcaagcaa cggtaacgtg cggtttaaac attttgcatc
    10381 cttaatctgt cacttctcgc cattcgccat tggcaagatt atccaaagag taatctccca
    10441 tcgcatagcg aatcagtcgc agcgtgggga aaccaacatg ggcggtcatg cggcgcacct
    10501 ggcgattacg tccttcatat aaggtgatct tcagccagct ggtgggaata cttttgcgtt
    10561 cacgaattgg tggattccgc ggccataacc acgcgggttc gtcaaccagc tccgcgcctg
    10621 cgggcagggt agggccatca tttaaggtta cgccattgcg caaggcttca agtgcgtctt
    10681 gtgtgggaat accttccacc tgcacataat agattttccc ggtgcgtttg cccggctggg
    10741 ttaaacgcgc ctgcaacgcg ccgttattgg tcagcaccag taacccttcg ctatcgcggt
    10801 caagacgacc tgctgcataa acgccctgaa ccgggatgaa ttcttttaat gttttgcgtc
    10861 cggcttcatc ggtgaactgc ggaagaacat cgtagggttt attgaacagg atcacacgcg
    10921 tgggctggtt ttcaggttta cgcctggtag aacgttgcga gctgaatcgc ttaacttggt
    10981 gatttctaaa agaagttttt tgcatggtat tttcagagat tatgaattgc cgcattatag
    11041 cctaataacg cgcatctttc atgacggcaa acaatagggt agtattgaca agccaattac
    11101 aaatcattaa caaaaaattg ctctaaagca tccgtatcgc aggacgcaaa cgcatatgca
    11161 acgtggtggc agacgagcaa accagtagcg ctcgaaggag aggtgaatgg aaagtaaagt
    11221 agttgttccg gcacaaggca agaagatcac cctgcaaaac ggcaaactca acgttcctga
    11281 aaatccgatt atcccttaca ttgaaggtga tggaatcggt gtagatgtaa ccccagccat
    11341 gctgaaagtg gtcgacgctg cagtcgagaa agcctataaa ggcgagcgta aaatctcctg
    11401 gatggaaatt tacaccggtg aaaaatccac acaggtttat ggtcaggacg tctggctgcc
    11461 tgctgaaact cttgatctga ttcgtgaata tcgcgttgcc attaaaggtc cgctgaccac
    11521 tcctgttggt ggcggtattc gctctctgaa cgttgccctg cgccaggaac tggatctcta
    11581 catctgcctg cgtccggtac gttactatca aggcactcca agcccggtta aacaccctga
    11641 actgaccgat atggttatct tccgtgaaaa ctcggaagac atttatgcgg gtatcgaatg
    11701 gaaagcagac tctgccgacg ccgagaaagt gattaaattc ctgcgtgaag agatgggggt
    11761 gaagaaaatt cgcttcccgg aacattgtgg tatcggtatt aagccgtgtt cggaagaagg
    11821 caccaaacgt ctggttcgtg cagcgatcga atacgcaatt gctaacgatc gtgactctgt
    11881 gactctggtg cacaaaggca acatcatgaa gttcaccgaa ggcgcgttta aagactgggg
    11941 ctaccagctg gcgcgtgaag agtttggcgg tgaactgatc gacggcggcc cgtggctgaa
    12001 agttaaaaac ccgaacaccg gcaaagagat cgtcattaaa gacgtgattg ctgatgcatt
    12061 cctgcaacag atcctgctgc gtccggctga atatgatgtt atcgcctgta tgaacctgaa
    12121 cggtgactac atttctgacg ctctggcagc gcaggttggc ggtatcggta tcgcccctgg
    12181 agcaaacatc ggtgacgaat gcgccctgtt tgaagccacc cacggtactg cgccgaaata
    12241 cgccggtcag gacaaagtaa accctggctc tattattctc tccgctgaga tgatgttacg
    12301 ccatatgggc tggactgaag ccgcagacct gattgttaaa ggtatggaag gcgcaatcaa
    12361 tgcgaagacc gtaacctatg acttcgagcg tctgatggaa ggcgctaaac tgctgaaatg
    12421 ttcagaattt ggtgatgcga tcatcaagaa catgtaatca ctacatgtgt ttaatattgc
    12481 aacgggcgta taacacgccc gttgttttat ttatgtaggt attattaata gcatatcgag
    12541 catatttata taaaaccctt tacttgagcc catatgggca tatttttata atgcaactat
    12601 tatgcaaaca tttatttgtt attttgcttt ctcctggagg acactcttga ctgcttttga
    12661 gt
//
LOCUS       DAZFJF010000123        12575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFJF010000123 DAZFJF010000000
VERSION     DAZFJF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.123"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            229..1116
                     /gene="rhtA"
                     /locus_tag="ACHKAU_003941"
     CDS             229..1116
                     /gene="rhtA"
                     /locus_tag="ACHKAU_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO5532917.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            1415..1918
                     /gene="dps"
                     /locus_tag="ACHKAU_003942"
                     /gene_synonym="pexB"
     CDS             1415..1918
                     /gene="dps"
                     /locus_tag="ACHKAU_003942"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO5532918.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            2322..3068
                     /gene="glnH"
                     /locus_tag="ACHKAU_003943"
     CDS             2322..3068
                     /gene="glnH"
                     /locus_tag="ACHKAU_003943"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO5532919.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            3207..3866
                     /gene="glnP"
                     /locus_tag="ACHKAU_003944"
     CDS             3207..3866
                     /gene="glnP"
                     /locus_tag="ACHKAU_003944"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO5532920.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            3863..4585
                     /gene="glnQ"
                     /locus_tag="ACHKAU_003945"
     CDS             3863..4585
                     /gene="glnQ"
                     /locus_tag="ACHKAU_003945"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO5532921.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            4702..6927
                     /gene="ybiO"
                     /locus_tag="ACHKAU_003946"
     CDS             4702..6927
                     /gene="ybiO"
                     /locus_tag="ACHKAU_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO5532922.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(6924..7850)
                     /gene="rlmF"
                     /locus_tag="ACHKAU_003947"
     CDS             complement(6924..7850)
                     /gene="rlmF"
                     /locus_tag="ACHKAU_003947"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO5532923.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            8126..8386
                     /gene="mcbA"
                     /locus_tag="ACHKAU_003948"
     CDS             8126..8386
                     /gene="mcbA"
                     /locus_tag="ACHKAU_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO5532924.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            8650..10932
                     /locus_tag="ACHKAU_003949"
     CDS             8650..10932
                     /locus_tag="ACHKAU_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO5532925.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            10974..11651
                     /gene="ybiX"
                     /locus_tag="ACHKAU_003950"
     CDS             10974..11651
                     /gene="ybiX"
                     /locus_tag="ACHKAU_003950"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO5532926.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            11725..11991
                     /gene="ybiI"
                     /locus_tag="ACHKAU_003951"
     CDS             11725..11991
                     /gene="ybiI"
                     /locus_tag="ACHKAU_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO5532927.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKQAYTGYNRRGSKDSQLR"
     gene            12256..12516
                     /gene="ybiJ"
                     /locus_tag="ACHKAU_003952"
     CDS             12256..12516
                     /gene="ybiJ"
                     /locus_tag="ACHKAU_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO5532928.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         3060 a         3239 c         3164 g         3112 t
ORIGIN      
        1 ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac gcatgttttc
       61 cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca atcctggcgc
      121 attttagtca aaacggggga aaattttttc aacaaatgct caaccatcat tgggtatatc
      181 cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat gcctggttca
      241 ttacgtaaaa tgccggtctg gttaccaata atcatattgc tcgttgccat ggcgtctatt
      301 cagggtggag cctcgttagc taagtcactt tttcctctgg tgggcgcacc gggtgtcact
      361 gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc atggcgactg
      421 cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct gggtgggatg
      481 aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt ggcgctggag
      541 ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga tttcgtctgg
      601 gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga cgtttcccat
      661 gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc tatttacatt
      721 ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtggcaat tggttcgttg
      781 attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc actctggcac
      841 tggtcggtta ttccattggg tctggctgtc gctattctct cgaccgctct gccttattcg
      901 ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct gatgagcatg
      961 gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact gacacccata
     1021 cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct gacagtacgc
     1081 aaagagagca aaataaaaga attagacatt aattaaattt acatttctgc atggttatgc
     1141 ataaccatgc agaatttctc gctacttttc ctctacaccg tctttatata tcgaattatg
     1201 caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt gttcttatca
     1261 atatatctaa ctcattgaat ctttattagt tttgtttttc acgcttgtta ccactattag
     1321 tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta gtctcgttaa
     1381 ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat tagttaaatc
     1441 aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga aaaaagcaac
     1501 agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga ttaccaaaca
     1561 agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc tggatggctt
     1621 ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgcagtgc agctgggcgg
     1681 tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa gttacccgct
     1741 ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg caatcgtcgc
     1801 taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag atatcctgac
     1861 cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca tcgaataaat
     1921 ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga caggaggccg
     1981 ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct atctccagct
     2041 gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag attgtttttt
     2101 catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg caccagattg
     2161 gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc acgtttttgc
     2221 cgcatctcga aaaatcaatg agttgcaaaa ctggcacgat tttttcatat atgtgaatgt
     2281 cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct gtattaaaag
     2341 tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg gataaaaaat
     2401 tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc gataaatatg
     2461 tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg gattacgaac
     2521 tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac gtcgatctgg
     2581 cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc tctgacggct
     2641 actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg aaaagcgtga
     2701 aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt gattacgcga
     2761 aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac gcctatatgg
     2821 aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt ctgtacttca
     2881 tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa gcgcagcaat
     2941 acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac ggcgcgttga
     3001 aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc ggtactgaac
     3061 cgaaataata acgctacacc tgtaaaacgc actggcagtt ccctctcccc tctggggaga
     3121 ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga atttgattat
     3181 ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc tggcctgcca
     3241 ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc ggtctggcag
     3301 gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt tggatagcca
     3361 accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc gtccaggtga
     3421 tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac ccatttactg
     3481 cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc acgcgtggtg
     3541 cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt ctttcacgtt
     3601 gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg ccgccgctgg
     3661 gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgtgatc ggcgtggcgg
     3721 aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt gagatctgga
     3781 gcgccgtggc ggtgctctat ctgattatta ccctggtgct gagctttatt ctgcgtcgtc
     3841 tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc actttggccc
     3901 aacccaggtg ctgcataata tcgatttgaa cattgcccag ggcgaagtcg tggtgattat
     3961 cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac tggaagaaat
     4021 cacctccggc gatctgattg tcgatggcct gaaggttaac gatccgaaag ttgacgagcg
     4081 cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc cgcatctgac
     4141 ggcgctggaa aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca aagaagaggc
     4201 ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg cacatcacta
     4261 cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg cgctggcggt
     4321 gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg aactgcgcca
     4381 tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg tgatcgtgac
     4441 ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg acaaaggccg
     4501 gattgcggaa gatggcgatc cgcaggtatt gatcaagaac ccgccgagcc agcgcttgca
     4561 ggaatttttg cagcacgtct cttaacttct ctgcctccct ttcacccgaa agggaggcac
     4621 accagattcc tctcatttaa aatcgcccct cccccagcat ctatacttat ctttttgctc
     4681 tgttttctca ctggaggagt catgcggtgg atcctgttca tcctcttctg cctgctgggc
     4741 gcacctgccc acgcggtatc catacccggc gttacaacca caacgacaac ggactcaacg
     4801 actgaaccgg ccccggaacc ggatatcgaa caaaaaaaag cggcctatgg cgcactggcg
     4861 gatgtgctgg ataacgacac ctcgcgtaaa gaattgatcg accagttgcg tactgtcgcc
     4921 gcgacgcccc ctgcggaacc ggtcccgaag atcgtgccgc cgacgctggt tgaagagcaa
     4981 accgtgctgc aaaaggtcac cgaagtcagc cgccattatg gtgaagccct ttccgcccgc
     5041 ttcgggcaac tttatcgcaa tatcaccggc tccccgcata agccatttaa tccacaaacc
     5101 ttcagcaatg cgctgagcca tttttcaatg ttagcggtat tagtgtttgg tttttactgg
     5161 ctgattcgcc tgtgcgcact gccgctgtat cgcaaaatgg gtcagtgggc gaggcaaaaa
     5221 aatcgtgagc gcagcaactg gttgcagctt ccggcgatga ttatcggggc gtttattatc
     5281 gacctgctgt tactggccct gacattattt gtcggccagg tattaagcga caacctgaat
     5341 gcaggcagtc gcaccatcgc tttccaacaa agtttgtttc tcaatgcctt tgccctgatt
     5401 gaatttttca aagccgtact acgcctgatt ttttgcccaa acgtggcgga gctgcgtccg
     5461 ttcacgattc aggacgagag cgcccgttac tggagtcgtc gcctgagctg gttaagcagt
     5521 ttgataggtt atggcctgat tgtggccgtg ccgattatct ctaatcaggt gaatgtacag
     5581 ataggtgcgc tggcgaacgt catcattatg ctgtgcatga ccgtctgggc gttgtacctg
     5641 atctttcgta ataaaaaaga gattacccag catttgctca actttgcgga gcattcgctg
     5701 gcctttttca gcctgtttat ccgcgccttt gcgctggtgt ggcactggct ggcaagcgcc
     5761 tattttatcg tgctgttttt cttttcgttg ttcgatccgg gcaacagcct gaaatttatg
     5821 atgggtgcaa cggtgcgcag cctggcgatt attggtatcg cagcgtttgt ttccggtatg
     5881 ttttcccgct ggctggcgaa aaccatcacc ctctcgccac atactcagcg taactatccg
     5941 gagctacaaa aacggctgaa tggctggctt tcggcggcgc tgaaaacggc gcgtattctg
     6001 accgtctgcg tggcagtaat gttgctgttg agcgcatggg gattgttcga tttctggaac
     6061 tggctgcaaa acggcgcggg gcagaaaacc gtagatatcc tgatccgcat cgcactcatt
     6121 cttttcttct cggcggttgg ctggacggtg ctcgccagtt tgatcgaaaa ccggctggct
     6181 tcggatattc atggccgccc gctacccagc gcccgtacgc gtaccctgct gacgctgttt
     6241 cgtaacgcgc tggcggtgat tatcagtacc atcaccatca tgattgtgtt gtcggaaatc
     6301 ggcgtcaata tcgcgccatt gctggcaggt gccggggcat taggtctggc tatctcgttt
     6361 ggttcgcaaa cgctggtgaa agatatcatt accggggtgt ttattcagtt tgaaaacggc
     6421 atgaacactg gagatttggt gactatcggg ccgttgaccg gcacagtgga acggatgtcg
     6481 attcgctccg tgggcgtgcg ccaggatacc ggggcgtatc acatcattcc gtggtcttcg
     6541 ataaccacct ttgctaactt cgtccgcggt attggttcgg tagtggcaaa ctacgatgtt
     6601 gatcgccatg aagatgctga taaagccaat caggcactga aagatgcggt agcggaattg
     6661 atggaaaacg aagaaattcg cgggctgatt attggtgaac cgaattttgc cgggattgtc
     6721 ggcttaagca ataccgcgtt tacactgcgt gtttcgttca ccacgctgcc actcaaacag
     6781 tggacggtac gctttgccct cgacagccag gtgaaaaaac atttcgacct ggcgggcgtt
     6841 cgcgcgccag tgcagactta tcaggtgctg cctgctccgg gcgcgacccc ggctgaaccg
     6901 ccgccgccgg gggagccaac gctttaacgt tggcgattaa caaaacggcg gcgctgctcg
     6961 tcgttcataa aggtccaggc aataaagcga ctctgctttt gcccctgggc catctctttt
     7021 tttaccacct tcaccgcgcc cacgtcggtc agagcacgat acaacggcgg taagttttca
     7081 ccacgagata ccagcgaggt aaaccacatc acctgcttcg cgaagccttt gctctcttca
     7141 atcatctttt tgataaaggc gacttcacca ccttcacacc acaactcctg ttgctgacca
     7201 ccaaagttca gtgcatcgtc tttgttcagc cccaggttac ggcgtttacg ctcactacct
     7261 gcccgcgctg cagcggcgga atcgtggaat ggcgggttac acaaggtcgc gtcgtattgc
     7321 tcgtttttat ggatgatgcc gttaaaaatt gccccactct ctttttgccg acgcagacga
     7381 atggcgcggt taagccccgg attagcactg ataatcgcct gcgcactgct taacgcctgg
     7441 ctgctggttt cactaccggt aaaacgccag ccatattcat gtacgccaat cagcggataa
     7501 atacagttcg caccaacgcc gatatccaga atgctggcat tcgctggaat tgttccgctt
     7561 gcttcagcca gtaaatcggc aaggtgatga atataatccg cccggcccgg tactggtggg
     7621 cagagaaaac cgtcggggat atcccagttc gctacggcgt aaaaatgggc cagcaacgcc
     7681 ttattgagcg ccttcaccgc cagcggattg gcaaagtcta cgctttgctc cccggcgggt
     7741 gtaagcgtga ggaattgcct gagttcagga ttgacctgac aaagcgtggc gagatcgtag
     7801 cggctgtgat gacggttgcg cggatgcaac cccggtttct gggcggacat agcattctcc
     7861 tttaagcagc ggcgtaagat acccgcataa cgcgacaggg taaatatctg gctgcgttta
     7921 tcattcccca aaaccttaac atggctatgg tttatggcaa ttcatcataa aaatcagcat
     7981 tatgcatgtc attgatttgt cttgttttgt aaaaatatat cgttcaaaaa ataagcacat
     8041 ctctgtcaat tttttgcgtc attttcatcg cccaggcgac ctacacttat caggcactac
     8101 ccgttgtatt tatgaggatg tcattatgaa aaagtgcctc acactactga ttgccaccgt
     8161 cctgagcgga atctctctca cggcttatgc cgcgcaaccg atgagtaacc tggacagcgg
     8221 tcagctacgg cccgccggta ccgtttcggc aactggcgca tcaaacctaa gcgatctgga
     8281 ggataaactg gcagaaaaag cgcgcgagca aggcgcgaaa ggttatgtca ttaattccgc
     8341 aggcggaaat gaccagatgt tcggtactgc aaccatctac aaataacccc accaacgtaa
     8401 ctgcattcgc ctctggatgc agttttcgcc tgttttgtaa tcaaaaaaca tcaaatatga
     8461 acgcaatgta aataaatgta tttctttttc gcgcaatggg tgatagaaaa tcgcgccaag
     8521 tgataatgct tatcaaaatt attatcactt gcacgaacac tatcacgaga ttaacagtgg
     8581 catcgcatcc gcagagaggc tttctcgtgg cagtgaaaat ttcaacatat aaaaaaagtc
     8641 acctgcaaaa tggaaaacaa tcgcaatttc cctgccagac aatttcattc gctcacgttc
     8701 tttgccggtc tttgtattgg catcacgcct gtggctcagg cactcgccgc cgaagggcaa
     8761 actaacgcgg atgacacgct ggttgtcgaa gcatcaacgc cttcgcttta tgcgccacaa
     8821 caatctgccg atccgaaatt ctcgcgtccg gtagcggata ctacccgcac gatgacggtg
     8881 atttctgaac aagtgattaa agatcagggc gcaaccaacc ttaccgacgc gctcaaaaac
     8941 gtccccggcg tgggtgcgtt ttttgcgggt gagaacggta actccaccac tggcgacgcc
     9001 atttatatgc gcggtgctga tacctctaac agtatttata ttgatggcat tcgcgatatc
     9061 ggcagcgtct cgcgcgacac cttcaatact gagcaggtcg aagtgattaa agggccttcc
     9121 ggcaccgact acgggcgcag cgcgccgacg ggctcgatca atatgatcag caagcagccg
     9181 cgcaatgatt ccggcattga cgcctccgcc agtattggca gcgcctggtt ccgccgcggc
     9241 acgctggacg tcaatcaggt cattggtgat accactgcgg tgcgcctgaa tgtgatgggc
     9301 gaaaaaacgc acgatgccgg acgcgacaaa gtcaaaaatg agcgttacgg cgtcgcccct
     9361 tctatcgctt ttggccttgg tacagcgaat cgtttgtatc ttaattatct gcatgtcacc
     9421 cagcacaaca cgccagacgg cggcattccg accatcggtt tgccgggtta ttctgcccca
     9481 tctgcgggaa cagcggctct gaatcattcc ggaaaagttg atactcataa cttttacggc
     9541 acggattccg attacgacga ttcgaccacc gacaccgcca ccatgcgttt tgagcacgac
     9601 atcaacgata acaccaccat tcgcaatact acccgttggt cgcgcgtaaa gcaggattac
     9661 ctgatgacgg cgattatggg cggggcgtcg aatattactc agcccaccag cgatgtgaat
     9721 agctggacct ggtcacgcac ggcgaatacc aaagatgtga gtaataaaat tctcaccaac
     9781 cagaccaacc tgacctcgac gttctatacc ggttctatcg gtcatgatgt cagtaccggc
     9841 gtggaattta cccgtgaaac gcagactaac tacggcgtta atccggtgac gttacccgcg
     9901 gtaaatattt atcatcctga cagcagcatt catcccggcg gcctgacgcg caacggcgca
     9961 aacgccaatg gtcagacgga taccttcgca atttacgcct tcgatacgct gcaaatcacc
    10021 cgtgattttg agctgaacgg cgggatccgt ctggataatt atcatactga atatgacagt
    10081 gccaccgcct gcggcggcag cggacgcggt gccatcacct gcccaactgg tgtggcaaaa
    10141 ggttctccgg tcaccaccgt cgacaccgcc aagtcgggca atctggtgaa ctggaaagcc
    10201 ggggcgctgt atcacctgac ggaaaacggt aatgtctata ttaactatgc cgtttcccag
    10261 cagcctccgg gcggcaacaa cttcgccctt gcgcagtctg gcagcggtaa cagtgccaac
    10321 cgcaccgatt ttaaaccaca aaaagccaac accagcgaga ttggcaccaa atggcaggtt
    10381 ctggataaac gcctgttgct caccgccgcg ctattccgta ctgatatcga aaatgaagtt
    10441 gagcaaaatg atgacggaac ttactcgcaa tacggtaaga aacgtgtcga aggctatgag
    10501 atatccgtgg ccgggaatat cacgcccgcg tggcaggtga ttggcggcta tacccagcaa
    10561 aaagcaacca tcaaaaacgg caaagatgtt gcccaggatg gttcctcatc gctgccgtat
    10621 accccggagc acgccttcac cttatggagc cagtatcagg caacagatga tatctctgtt
    10681 ggcgcgggcg cacgctatat cggcagtatg cataaaggtt cagacggcgc ggtgggaacg
    10741 ccagcgttta ccgaaggtta ctgggtcgcc gatgccaaac tggggtatcg ggttaatcgc
    10801 aatctcgact tccagctaaa cgtttacaac ctgtttgata ccgattacgt cgcctcaatc
    10861 aacaagagcg gctaccgtta tcacccgggc gagccaagaa ccttcttgct cacagccaat
    10921 atgcatttct gattcagatg tggggcgcag gccccacttt ttggagaact tgtatgatgt
    10981 accacattcc cggcgtgtta tcgccacagg acgtcgcccg gtttcgcgaa caactggaac
    11041 aagccgaatg ggtggatgga cgtgtcacca ccggcgcaca aggtgcgcaa gttaagaaca
    11101 atcaacaggt cgacacccgc agcacgttat acgccgccct gcaaaatgag gtgctgaacg
    11161 cggttaacca acatgcttta ttctttgccg cggccttgcc gcgtaccctt tccacgccgc
    11221 tgtttaatcg ctatcagaac aatgaaacct atggtttcca tgtggatggc gcagtacgca
    11281 gccatccaca aaacggctgg atgcgtactg acctttctgc cacgctgttt ttaagcgatc
    11341 cacaaagcta cgacggcggc gaactggtcg ttaatgacac cttcggacaa catcgggtaa
    11401 aactcccggc aggcgatctc gtgttgtatc cctccagcag cctgcattgc gtgacacccg
    11461 taacccgcgg cgtacgagtg gcatcattta tgtggatcca gtcgatgatc cgtgatgata
    11521 aaaagcgcgc catgctgttt gaactggaca acaatattca gtcgctgaaa agccgctacg
    11581 gcgaaagtga agagatcctg tcgctgctta atctttatca taatctgctg cgggaatggt
    11641 cggagatctg atgcttgaac tgcccaggtt cgtccctgga ttagctacac ttaactgtac
    11701 aagcattgat atggggagga cgatatggca tccggttggg ctaacgatga cgccgtcaac
    11761 gaacagatca acagtacaat tgaagatgcg attgcccgcg ctcggggtga aattccgcgt
    11821 ggcgaaagcc tggatgaatg tgaagagtgc ggtgccccca tcccgcaagc ccgtcgggaa
    11881 gccattcctg gcgtgcgctt atgcattcat tgtcagcagg agaaaggttt acaaaaacaa
    11941 gcgtatacag gatataatcg cagaggttcg aaagacagcc agttacgtta actacaactg
    12001 gcgagatgca tagcgagtat aatttgtatt ttgcgtggca ttccgtgcct ttaacgctgc
    12061 tgtgactgcc acgcggcata aaacaagtcc ataagtacaa caaataaatg gtttatcagt
    12121 atgttagata tcaatcaatt tatttgaaca aggcggtcaa ttctcttcga ttttatctct
    12181 cgtaaaaaaa cgtgatactc atcacatcga cgaaacaacg tcacttatac aaaaatcacc
    12241 tgcgagagat taattatgaa aactatcaat actgttgttg ctgctatggc tctttcaact
    12301 ctgtcatttg gcgtgtttgc cgcggaaccg gtaacggcat cccaggcaca gaacatgaat
    12361 aaaatcggcg tggtttctgc cgatggcgca tccaccctcg atgccctgga agcgaaactg
    12421 gctgagaaag ccgcggcagc cggtgccagt ggatacagca tcacttccgc caccaacaac
    12481 aataaattaa gcggtactgc ggtaatttat aagtaattcg taggacggat aaggcgttta
    12541 cgccgcatcc ggcaacagat gcctgatgcg acgct
//
LOCUS       DAZFJF010000124        12542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFJF010000124 DAZFJF010000000
VERSION     DAZFJF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.124"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            130..747
                     /gene="yijF"
                     /locus_tag="ACHKAU_003953"
     CDS             130..747
                     /gene="yijF"
                     /locus_tag="ACHKAU_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO5532929.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHKAU_003954"
     CDS             complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHKAU_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO5532930.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHKAU_003955"
     CDS             complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHKAU_003955"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO5532931.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHKAU_003956"
     CDS             complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHKAU_003956"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO5532932.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5248..5382
                     /locus_tag="ACHKAU_003957"
     CDS             5248..5382
                     /locus_tag="ACHKAU_003957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532933.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHKAU_003958"
     CDS             complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHKAU_003958"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO5532934.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHKAU_003959"
     CDS             complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHKAU_003959"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO5532935.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9393..9710
                     /gene="metJ"
                     /locus_tag="ACHKAU_003960"
     CDS             9393..9710
                     /gene="metJ"
                     /locus_tag="ACHKAU_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO5532936.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHKAU_003961"
     CDS             9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHKAU_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO5532937.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10647..11322
                     /locus_tag="ACHKAU_003962"
                     /pseudo
     CDS             10647..11322
                     /locus_tag="ACHKAU_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(11409..11731)
                     /locus_tag="ACHKAU_003963"
                     /pseudo
     CDS             complement(11409..11731)
                     /locus_tag="ACHKAU_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
     gene            complement(11733..>12542)
                     /locus_tag="ACHKAU_003964"
     CDS             complement(11733..>12542)
                     /locus_tag="ACHKAU_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5532938.1"
                     /translation="VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISKEGTTEWCAE
                     YDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKG
                     GWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQ
                     GEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSL
                     NFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT         3089 a         3442 c         3060 g         2951 t
ORIGIN      
        1 tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg cttctggaca
       61 ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg
      121 acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac
      181 tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt
      241 gccatcgcag acggtgccag acagcaaatt ggcagcacgc tattttacga tccggcgtat
      301 atgcagctta cctatcctgg cggtgatgtc ccgcaagaac gcggggtatg ttccgatgtg
      361 gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggtgca cgaagatatg
      421 gcgaagaatt ttgccgagta cccgcaaaaa tggcagttaa agcgcccgga cagcaacatc
      481 gatcaccgtc gggtgcctaa tctggaaacc tggtttaccc gccacgataa aacacgcccc
      541 atcagcaaga accccagtga ctatcaagcg ggcgatattg tctcctggcg actggataac
      601 ggactggcac atattggggt ggtgtcagat ggcttcgccc gcgacggtac gccgctggtg
      661 atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg
      721 gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa aattcagatg
      781 ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc
      841 agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag
      901 agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc aggcaagttt
      961 ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc actgtaggcc
     1021 agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc
     1081 acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgacaa taaatccacg
     1141 cgcggatgac gggcatacag acgtttagca acaatcgcgc tcgcccccca actgacgccg
     1201 gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac
     1261 accaaaaata agccgaaagc ggcaatcagt atcgcgaaat attgcccacg tcgcaggcgt
     1321 tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata gcttaggatc
     1381 gccaccttcc ccgctccgcc gctgaccagc gcccactgcg ccaggccaac catcccgcag
     1441 gtttgtaaca gggcaatggc taaggtgtat ttaaacggtg tcgggcgcat tccgcgacca
     1501 cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag
     1561 tcgaaggcac cgatgtagct ggtgacttgc ttcatgaaaa tccagctata actccagata
     1621 agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc gacagacatg
     1681 agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac
     1741 tgcctgaaag gcagtcgctg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc
     1801 atcactttca cccatgccgc cacgaagtct ttaacaaact tctcgtgagc atcgctgctg
     1861 gcgtaaactt cagccaccgc acgcaggaca gagttagaac caaataccag atccgcacgg
     1921 ctggcagtgt atttcacttc gccggtttcg cggtcacggc cttcaaacag ctctttcgat
     1981 tcgtcggtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc
     2041 aatacgccaa cgcggtcagt gaagacgccg tttttgctgc catcgaagtt ggcacccagt
     2101 acacgcatcc cgcccaccag cgcggtcatt tccggcgcgg tcagggtcag ttgctgggct
     2161 ttgtcgatca gcagtgactc ggtggtggaa acatccagac gagcgcgata gttacggaaa
     2221 ccgtcagcaa ttggctccag cagctcgaac atctcaatgt cagtctgatc ctgacgcgca
     2281 tcaacgcggc ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc
     2341 tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct
     2401 ttctggattt tctccaaaac aggcagagca cgaacggctg cggcgttcac atcccagtcg
     2461 cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg
     2521 aaggtagaag cagaagccca ggccaccgat accagctcac taacagacag accagaatcc
     2581 gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc
     2641 agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc
     2701 ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc
     2761 tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc
     2821 aggtcggtca ccagcatcgt cggtttacgt ttcttcgacg gatcaaacgg atccgggata
     2881 atttccggtg cgtctaccgc ttcgaactgg attgcgccag ccgggctgcg ggtctgtacc
     2941 cactcatact tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc
     3001 cagactactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc
     3061 gcccaaccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacatttgat
     3121 gtcggaccgg caccgtgggt tttacccagc gtatgaccac ccgcaataag cgccacggtt
     3181 tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc cgcagaaagc
     3241 ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcaggcccat ctcggttgca
     3301 cccagcggtg ctttcgccag cgcttccgga tgacggtggg tcagccaggc tttttcatca
     3361 ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag
     3421 gtacggaagc cggagttttc cagcgccacg ttacccgcga ggataaacag gtcggcccag
     3481 gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcgagg
     3541 cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg
     3601 ccgcgtccat cgattgaacg gtaagtcccc gcgccgtgcc aggccatacg aataaacaga
     3661 ccggcgtagc tgccccagtc ggcaggccac cacggctgag attctgtcag cagggctttc
     3721 agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc
     3781 tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt
     3841 ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg
     3901 aacgggcatt tgccagtggc tgtcgtgtta tgaatatcgt ctgacgtgct catcaatgtg
     3961 ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt
     4021 aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat gtagatcaaa
     4081 ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaactgctt tataaaaaag
     4141 ggttttagat tcctattctt caaggaagga gggcgaaaac aaagctgaca accttatttt
     4201 tacgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cacagtgtga
     4261 ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc
     4321 gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct
     4381 taaaatcttc accatatcca tggcaatatt cgcgcccacc agcttgcgcg tttcagcatc
     4441 gtcatccagc ccgtcaaaca tttgtgccat ccacgccgga atacgcacgt tggtcatatc
     4501 ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa atccccggaa taatttccac
     4561 atcaatgccc gccgatacgc agcggtcacg aaaacgcagg tagctttcga catcaaagaa
     4621 gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcaggt tgagcaaatc
     4681 cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg agatatcgaa
     4741 atctgccact tcttttaaca gcgtcaccag gtcagaagca tacatttccg gcttaccact
     4801 tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat tccagtagtc
     4861 gcgtgcaatg gtgcgcagct cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc
     4921 ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc
     4981 ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat
     5041 ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac
     5101 gttaatctgc ccctggactt ctgccaggct ctgattcagg gcatcccgct ggctggcgtg
     5161 aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc
     5221 tatccattta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa
     5281 atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa
     5341 aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt
     5401 gcttcttgcc ggattcgtcg taaacgcttc atccggccta atcctatgat aattcaatat
     5461 atttcattag aaaccataga cccgaaaaca tgagtaccgg gcattattaa atttctgaaa
     5521 ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg
     5581 cccagcgcca ggcccacgga tcaccagcgg attatcgcgg taccagcggc tttcgatggc
     5641 aaagacgtta tcgcatggca gcagtgatgc cagcggatgg tcttcacgca ccgcttccac
     5701 gcccacacgc gctttgccat tggcatcgaa acgcgccacg tagcgcagca ccagccccat
     5761 ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa
     5821 gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg
     5881 gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc gcattacgtc
     5941 tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc
     6001 caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc
     6061 ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat cgcgcacggt
     6121 gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac gcccggtttt
     6181 ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg ccagtttgtt
     6241 ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag caagctgctg
     6301 gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca tccacaggaa
     6361 caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg cgcggctggc
     6421 gtccagcccg tcatagctca acaggctgcg gcggctgtcc accacacctg ccagcacaaa
     6481 ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt ccagccaacg
     6541 ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg cgcggaagac
     6601 ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag
     6661 gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca gttgctgcca
     6721 gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg ccaccagcgc
     6781 cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga ttttcagcgc
     6841 actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt tatgtacgcc
     6901 aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt tatgcgccag
     6961 tttgaaatcc tgactgacgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt
     7021 gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc
     7081 cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg gctgtaaagt
     7141 acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat ccagacgcag
     7201 caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac
     7261 gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg tcgcggaata
     7321 gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc tgataaatcc
     7381 ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca acgggtaaga
     7441 aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact cgcgggcatc
     7501 aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc gtgccgacca
     7561 tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc tgtcgagcag
     7621 cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcgg cttcttcagc
     7681 gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg
     7741 cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct
     7801 accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca taatgcccgc
     7861 gacacgcaaa taacacttca catcagccag actactgcca ccaaatttat gcagctgacg
     7921 acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag
     7981 ccattttcca agtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc
     8041 agcgtctcgg aaatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg
     8101 gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac
     8161 gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc aaaactcaac
     8221 attgcgccaa agcctttttg ctgacgcgcg gcaatttcat gcccctgatt ttccggcagc
     8281 gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc
     8341 gcgttgcgct gcgccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga
     8401 tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg cccaccaggc aagttcagtg
     8461 acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga
     8521 tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc
     8581 gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag
     8641 attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt
     8701 ttgggttttt ctgccagtgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac
     8761 acgcgatagc aaccgcgttt cgccaggctg tcgaacaggc gatagctacc gccgtagcag
     8821 tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcattac caggtgaatc
     8881 gcggacatgc cggtattagt aagtactgca ccagcaccgc cttccagttc tgccagcgca
     8941 cgctgaacca catcgcgcgt tgggttgccg cgacgcgagt aatcatgcgc gcgcggttca
     9001 ttaaatccgg taaagttata tgtgctggaa agatggatcg gtgggacaac gcaaccatac
     9061 tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga
     9121 tgaagttccc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg
     9181 gacgtcaata catctggaca tctaaacttc tttgcgtata gattgagcaa atcccaaata
     9241 gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg
     9301 cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg tggtctggtc
     9361 tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca
     9421 gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc
     9481 ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc
     9541 gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac
     9601 ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa
     9661 aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcggaaatg
     9721 agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt catattgcaa
     9781 tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa
     9841 acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa
     9901 acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccgcaaa
     9961 ctggcgacat catctttcaa atctctcgct catcgcaaag taaagcgatc caactggcaa
    10021 cccattccga ttacagccac accggtatgc tggtgatgcg taacaaaaag ccctacattt
    10081 ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggattgcc catggtgaaa
    10141 agggcaaata cgtcgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac
    10201 tggcgcaaac ggcaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg
    10261 acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc
    10321 gcgtgggcga acagcagaag ttgaaagagt ttgatctcag caacccgctg gtccaggcga
    10381 agctcaaaga acgctacggt aaaaatattc cgcttgagga aacggtagtt tcaccacaag
    10441 ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt
    10501 aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt agttgcacga
    10561 cctttccgca agctgtcgct ccggtaaacg gtgatgcctc caattagttg aattaaggat
    10621 ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc ccccgttgcc
    10681 agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga ttccgcggcc
    10741 gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa ccgggagtta
    10801 aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact gcccgtacgc
    10861 tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac gaagaataac
    10921 gtcatccccg gtcgctcatg ctgatgtggc actcatctgc aaacttagtc agcaatggag
    10981 ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca cgaaaactgg
    11041 tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg aattgcgggc
    11101 gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag ttacgctaga
    11161 gagttaccga aggagaagca tctgactggt aagattttta ctcagcacat tgagcgtaac
    11221 aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg tttctcgcgt
    11281 tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg ttctactaat
    11341 tggagtcatc acccttctga taagctttcc ttggatgatt accagactga atacacagcc
    11401 aaagattttt acctcattct gctctttttt ctcaaatgac atatattata ttttacatca
    11461 tattcatagg catatgacat tcccccgccc cccattatgt cattgtgggt tgatgttggg
    11521 ctttcataaa aaaaacatct ttttttgaac tagcattaat tatatttact ttatatatta
    11581 aaatattttc tccatctgga aatattttag ccacacaata taaagcagct gaatccgaat
    11641 tacttaaaaa tatattatca tcacccataa ctccagccat taaatgactg gagactaaag
    11701 caaggaaata acaacattgt attgccttca ttttaattac tcccttgcct gataattgta
    11761 actccattcc ctttagcttt gggattatat tttagatagg tgttatttgg tgttcccaag
    11821 taactactgt attctttttt tcccattcca ttcatataag aattcgttaa atctttgctt
    11881 gaaaaattta gactatcata aacatcattt tcttttgtta ctttatttcc tttatcgtca
    11941 gagtaaaaac cgtgagtgtg ataatcgcct actctctctg aaccttcagg gcattttata
    12001 tttcgcaagt ctacactatc attatcattt gaacttatcg gattcatggg gaaatattca
    12061 ccttgcttct tacatattag tccaccatat tctttattct cgttaattga ttctccatta
    12121 cacattgcca atgcaaatat tccaatatca tctatatttt ttgatttaaa ttcatataat
    12181 cctaatggat ccatgctatt caccgggctc agcggatatg tatacaggtt ccatcccccc
    12241 ttcagcccaa tcggatcctg agtgatatac cgcccctgca gcgggtcata atagcggtgg
    12301 cggttgtaat acaggccgga ctcctcatca tactgctgcc ccggcaggcg gataagctgc
    12361 tgcagctgat gcgggttctc ttcattcagc aggttgcccc attcatcgta ttctgcgcac
    12421 cattctgttg tcccttcctt gctgataagg gccagcggca ggccgcgatg gtcgcagtgg
    12481 tacaggtgga tttttcgcgc cggcgtgtac accgggtcca tctggttttg catctgctcc
    12541 ac
//
LOCUS       DAZFJF010000125        12410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFJF010000125 DAZFJF010000000
VERSION     DAZFJF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.125"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..830
                     /gene="yjiC"
                     /locus_tag="ACHKAU_003965"
     CDS             <1..830
                     /gene="yjiC"
                     /locus_tag="ACHKAU_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5532939.1"
                     /translation="SMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIYL
                     LPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKIT
                     SALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYAG
                     TTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGND
                     PIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(971..1744)
                     /gene="uxuR"
                     /locus_tag="ACHKAU_003966"
     CDS             complement(971..1744)
                     /gene="uxuR"
                     /locus_tag="ACHKAU_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO5532940.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(1959..3419)
                     /gene="uxuB"
                     /locus_tag="ACHKAU_003967"
     CDS             complement(1959..3419)
                     /gene="uxuB"
                     /locus_tag="ACHKAU_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO5532941.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFIGAVTAAYQQLCERGARECVAAL"
     gene            complement(3500..4684)
                     /gene="uxuA"
                     /locus_tag="ACHKAU_003968"
     CDS             complement(3500..4684)
                     /gene="uxuA"
                     /locus_tag="ACHKAU_003968"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO5532942.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5136..5228
                     /locus_tag="ACHKAU_003969"
                     /pseudo
     CDS             5136..5228
                     /locus_tag="ACHKAU_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5471..6373)
                     /gene="fimH"
                     /locus_tag="ACHKAU_003970"
     CDS             complement(5471..6373)
                     /gene="fimH"
                     /locus_tag="ACHKAU_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5532943.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6393..6896)
                     /gene="fimG"
                     /locus_tag="ACHKAU_003971"
     CDS             complement(6393..6896)
                     /gene="fimG"
                     /locus_tag="ACHKAU_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO5532944.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(6909..7439)
                     /gene="fimF"
                     /locus_tag="ACHKAU_003972"
     CDS             complement(6909..7439)
                     /gene="fimF"
                     /locus_tag="ACHKAU_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO5532945.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7449..10085)
                     /gene="fimD"
                     /locus_tag="ACHKAU_003973"
     CDS             complement(7449..10085)
                     /gene="fimD"
                     /locus_tag="ACHKAU_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO5532946.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10152..10877)
                     /gene="fimC"
                     /locus_tag="ACHKAU_003974"
     CDS             complement(10152..10877)
                     /gene="fimC"
                     /locus_tag="ACHKAU_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5532947.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(10914..11453)
                     /gene="fimI"
                     /locus_tag="ACHKAU_003975"
     CDS             complement(10914..11453)
                     /gene="fimI"
                     /locus_tag="ACHKAU_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5532948.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11518..12066)
                     /gene="fimA"
                     /locus_tag="ACHKAU_003976"
     CDS             complement(11518..12066)
                     /gene="fimA"
                     /locus_tag="ACHKAU_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5532949.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3080 a         3215 c         2950 g         3165 t
ORIGIN      
        1 tgtctatgcc attaagcaat acccttcaga gccaaattat tacagataat cacttccttc
       61 atcatccaaa agtcgacagt gagttaacac gtaaatatga acgtgcacgc ctggatacgg
      121 aaaatatcta tcttctgcct cttgctcgtg gcaataacca taattacgat ggaaaaagcg
      181 ttgtcgaaat tagaaaactt gatattagta aagagtcctg gccatttaat tatgtgacag
      241 aagcatgcag agaatccgat ggtattacaa caacaggacg tatgctctat cgaaacttga
      301 aaatcacctc agctttagac gaaatatatg gtggcatatg taaaaaggcg catgctgcta
      361 cagaattagc agaaggttta cggctaaatt tatttatgaa gagccctttc gatcctgttg
      421 agaactatac agttcatgaa ataacattag gccctggttg caatgttcca ggttacgccg
      481 gaaccacgat aggatatatt tcaacccttc cagtttctca ggcaaaaaga tggaccaatg
      541 agcagccaag aattgatatc tatattgatc agataataac tgtctcaggg gtagcaaatt
      601 cttccggatt cgcccttgcc gcattactta atgctaatat tgagatggga aatgacccaa
      661 tcattggtat agaagcctat cctggtaccg cggagatcca tgcaaaaatg ggatataaag
      721 ttatccccgg tgatgaagat gcgccactca aacgcatgac tctgcaaccg tcatctcttc
      781 cagaactgtt tgaattaaaa aatggtgaat ggaattatat tggtaaatag ccactgagaa
      841 gaatgagtac taatattcag gttatacaga tatttaaaca ataccctgca atttacaaag
      901 caatactcat tgcgacttct taatttaaga aagcgcaagg aacgtttacc cttgcgctta
      961 ttataataag tcagacgtcg actttatcca gcggccatga atcaaacaga tagccgtcaa
     1021 aataaatatc gtcaacgttc gagaattcca gcagacgttg cttaacgttt tccagatgct
     1081 gccacattgc cagcttcgcc gctcgggcat cttttttgat taacgcggcg aggatctgtt
     1141 tgtgatcgcc caaccactct ttgcgataca ggctgtcatc cagatggctg tgcaactgga
     1201 tccacattgg attgttttcc cgccactgcc aggactgacg gaataactcc accagcatgc
     1261 tgttatgcgt tgcttcggca atagcgagat ggaactgcat gtcaccgctt tcgctgctgc
     1321 ccggcgcact ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga
     1381 tatcttcgcg ggtagcctgc aaagcggcaa actcggcgat gttgctctcc aataactgcc
     1441 gcgcctgtaa cagctcaaaa ggaccggcat cgttgcagac gttggcatcc ggactgtctg
     1501 tgttctggct gcctgagtta tcaagaacat agataccggc accccggcgt acttccacca
     1561 gccctttgat ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca
     1621 tttctgcaat ttcacgctcc ggcggcagcc gttcgccagg attgtacggc gtctttatga
     1681 tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtggcagatt
     1741 tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg
     1801 ccttatccgg cctacaacgg gcaattttac ggcccccgtt aatctcaaac tataccatta
     1861 gcactggaag ttggcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatca
     1921 gtttgattga ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg
     1981 cgcgcaccgc gttcgcacag ctgctgatat gccgccgtca ctgcgccaat aaagtcggca
     2041 ttctgcggca gatcatcggc aaaaataccg ctcaggccca gcagcgcttt cacgcggtct
     2101 gcgccctgat actgcgcgtt gaccttctgg aactccgcca gcatcgggtc gaccacgtca
     2161 atggcattac cctgctcatc cacgccctgg gtgtaacgca tccagccagc cacgcccagc
     2221 gccaggtgac gccagctgcc gccgttttgc aggtgcagac gcaccgggtc cagcagacgc
     2281 tgcggtaact tctggctgcc gtccatcgca atctgccagg tacggtgacg cagagacggg
     2341 ttgctgaaac gctcgatcag cagcgtcgca taggcgttca ggtccgtacc ttccggcatc
     2401 gacagcgttg gcgcttgttc ctgcatcatc aaggagaagg ccgctttgcg ataatccggg
     2461 ttagtcatgg tgtcggcaat ggtttcatag ccgccgaggt aaccgaggta tgccaggaaa
     2521 gagtggctgc cgttcagcat acgcagcttc atcatttcga acggcacaac gtctgcaacg
     2581 aactgtgcgc ccactttatc ccagtccggg cgaccgttaa cgaagttatc ttcaatcacc
     2641 cactgacgga acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca
     2701 atttcctgca aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg
     2761 aaggtgacgt tttcttcaat ccatgccgcc agctgcgggt cacgcgcctg agccagtccc
     2821 agtaccgcga ccttcgccac atggccgttt tcacgcacgt tatcgcagga catcaccgta
     2881 aacgctttca accctttttc acgacgcagc cgcagggctt cgacgatgta accaatcgcg
     2941 gacttcggcg cagtcgggtt ttccagatcg tgcttgatca gtgggttatt gagatccagc
     3001 tgaccgcttg ccgcatcagc gcagtagcct ttttccgtga ctgtcagaga gacaatcgcc
     3061 gtctgcggac gcgccatcgc attgagaata ccttcgcagc cgtcgatttc cggatgcagc
     3121 gcttctttca tcgaaccgat aattttcagc tcggtgcttt ccgcgccttt ttccgccacg
     3181 gtgtacagca gttgctgttt tttcaggttt tcgatcagca cgcggtcgtt gcctggcatc
     3241 aggttaactt cgcagatacc ccagtcgctg tcggtgcttt ccagcaggtg atgggtatac
     3301 agcgcctggt gcgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga
     3361 cgagaatgct cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc
     3421 gtaaactcca gaatccgtct tcagagagtg acggcaagga agaaggaacc ggcacggcgc
     3481 gcagccatgc cggtgggtat taacggctaa agaaagcgcg ctggatcgcc agttcgacac
     3541 cgcgaacttc ggccaggcct ttcagacgac caattgcgga gtaacctggg ttggttttct
     3601 tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt
     3661 tgccttccgc tttacgacgg tgttcttctt caacaatcgc tttcaccact tcgtacatat
     3721 caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg
     3781 tggagcgcag atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat
     3841 cgttgtcagc acgcacgccg taggaaccgg tgcacatggt gaaaccgttc gccatgctgt
     3901 ttacggtatc aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaggccaa
     3961 ggatcgggcg cggcggatcg tccgggtgaa ccgccatacg cacgccaact tcttcagcaa
     4021 ctggaataat cgctttcagg aagacggcaa agttttcgcg cagcttcgct ttatcgatat
     4081 ctttgtacag ctccaggtgc ttacggaact ggtcgagggt atacccttct tccgcgcccg
     4141 gcagaccggc aatgatgtta cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga
     4201 agcgaacagc agcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgtt
     4261 tcaggatatg catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc
     4321 cgtctggcag cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt
     4381 agcacacggt gcgaatgccg cactgcgcca ggttgcgcag agtctgctga tagttagcga
     4441 tccactgctc atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcta
     4501 cgacagacca caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta
     4561 cggaccatac ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg
     4621 cctgacggac atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt
     4681 ccatgatttt tcctctttca catagtgggt tccacagcaa agagacgcgg aaccctggag
     4741 ttgtttaggt atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg
     4801 gcgtataagt tctacctttt ggtaagacat gtcaacccga attctgaaat tggttaacca
     4861 catcacaaga atttcactct catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg
     4921 gttattgatc agaggatgtg acattcatcg caacaatggt tgaccaattt acataacatg
     4981 tcgcccaaaa taacaacggt tcaaccaggt aaggtaaaac gttatgcatg tgcttaacat
     5041 tctctgggtg gtattcggca ttggcctgat gctggtactg acaaagaccg caggttcaga
     5101 cacattcgct gcattagcaa tgcctcttag ataattttga cctgtcagta aaagacacgt
     5161 tgaaaacctg gggtctgctg gagctggtca actccgtggt tgggctgatt attagcaagg
     5221 tagcgtaaaa gaacaccaga gcctgccaat ggcaggctct gactgatgac aaagtctaaa
     5281 aatgtgcccg gacagtcccc tcgcccctcc ggggagaggg ttagggtgag gggaacagac
     5341 cagcaaagtg cgctttgttt atgccagatg cgacgctgac gcgtcttatc tggcctacaa
     5401 agggctaacg tgcaggtttt tagcttcagg taatattgcg taactgcatt agcaatgccc
     5461 tgtgatttct ttattgataa acaaaagtca cgccaataat cgattgcaca ttccctgcag
     5521 tcacctgccc tccggtacgt gcgtaatttg ccgttaatcc cagacttacc gccgaagtcc
     5581 ctactgctcc taacgatacc gtgttattcg ctggaataat cgtaccgttg cgcgtcaact
     5641 gtacgccgac gccctgcgct ggtgaaaacg acgcggtatt ggtgaaaatc gagttgcccg
     5701 catctacggt tgtgccggag aggtaatacc ccagattttg gcttttcgca caataaacgg
     5761 taagaggaat tggcactgaa ccagggtagt ccggcagagt aacggtgaca tcacgagcag
     5821 aaacatcgca gccgccagta ggcaccacca catcattatt ggcgtaaata ttccacacaa
     5881 actgaaaatc atcgctgtta tagttgttgg tctgtcgcaa aataagcacg gcaattaatg
     5941 agccagcttt aatcgccacc ccgcccgcac tgctcacagg cgtcaaataa agcgccaccg
     6001 gccacggctt atccgttctc gaattataaa caacgcgcgg cgtttcgctg gtggtaggaa
     6061 atggatcgct actgccacta tattttacgg tcccggaaaa attagataac acgctgccat
     6121 aagccgagcc tcgttgcagt gtgacatagt ctgtaatggt ttccggataa tcgttatggc
     6181 aaaagatttg cgtcgaaaga tccacgacca ggttttgccc cacattcacg gcgggcgcaa
     6241 ggtttacata aacattggcg ctgccaccgc caatagggat agcggtacca ttggcggttt
     6301 tacaggcgaa tgaccaggca tttaccgacc agcccatcag cagtacagca aacagggtaa
     6361 taactcgttt cattacaatc atctcttcgg gttcagctgt aggtataggt gatgctaatc
     6421 actgcctgaa tggttccctg agtggctccg ccatttaccg tcaatgctct gacctgtaac
     6481 gggaagtgcg ctgattgtga ggaatcatcc acctgaactg ttttggttgc gccagtattc
     6541 aatgtgttgc cactgtcatc ctgtagctct aactggatgt tttgcgcggt cccctggttt
     6601 ttataatatc cggtactgtc ggctgccccg ctgaagctgg cggtgaccct cgacgttccc
     6661 accggacaat tagtcaactc aagcgcaaca tcatgccagg ccgatgccgc cccggcagac
     6721 atcagactga aagaataaag atcgccgaga tcaaccgtgg cattggtggt ggaaaccgta
     6781 cacggtttgg cgacgacctt accgttcacc gtgatggtga catcggctgc ctgtatcgtc
     6841 gcacttgcga gcgccaatat tgccgccaat aaatacccac gtttgcgcca tttcatgagc
     6901 atctccagtt actgatattc aagagtgaag gtagccgtgg cattgatatg ccccgcagtg
     6961 acaggcacct gtgtcgccat tagccgggcg taaaaattca acgtatttgg tttacccggc
     7021 gtcagggtcg tccacgaaat cgcggacgat ggagcattaa ggggtatttg attttgctgc
     7081 tcattcagaa gctgtattcc cagtcccgaa gccgctgaca ccgtattttc aagtgcaagc
     7141 agattggcat tgtggctatc tgcaacgccg gtaaacccaa cttttacggc agaaacggca
     7201 ttaccacagg gtgacagcaa aatacgaaat ggaacaacag gagtcgtcgc gccaatgttg
     7261 ttaaattgct tcgccgcgtt ttccatcaga tcaacagtaa aattggttga ttcagcggcc
     7321 acactacagc cgttatctct gacatagccg cggatagtaa tcgtgctatc cgcagccaaa
     7381 gcgtgactca ccgccagcca caaaaaagcg cacagaagat aaaaaggttt gtttctcatc
     7441 acgccccctt aacgacattc agctgatagc tgggttaata actgctgctg actctctggt
     7501 ggcagttgat aattggcgac acagtgagca ttttcctctt ctccccattt cacctgaact
     7561 tttcccgcta aaggcattcc gctgaggtaa acctgaccat tatccgcaac aatgccgcta
     7621 ctctggctac tctctgatgt caccatcgcc ccaaacggca gcggcttatt attgtgggtt
     7681 agcgtcatga gcagttttat cccaacgcgc gctttaaact ctgctcgcac gatcgcccca
     7741 cgagtgggaa caacgttagc gaccgcgtta tctaaatcga cgttatcagc cagggtattg
     7801 gtatccagcg ccactctgtt ttccctatat tcagtggcat aaggcagcac ggcataaccg
     7861 cgccagtcgg tacgcacccc cgtctggttt tcgacttttg catcttttgc gccaggcgct
     7921 ttaacaagca ccaccgtatc gtttaacggc tgccccagcg ttacgccatt ggcatgagcc
     7981 aataccccac cgctgactcc gtaatagagc tgcttaatat catcgctatg gctgtaaccg
     8041 atattggcat tgccgtaacc accgcgataa ttcagcgtgg cgtagcctgt gcttccgcta
     8101 ttaccatcgc ctcccccggc atagccggtt tgcacgctat agctgaggtt gttgtcttcc
     8161 agcaacgtac cgtatacacc agccagattg gtcatccgac cgttgagatc gtgtgacatg
     8221 ctgtagctgg cactggcatg tcgccactga gatttactgt cagaacgcag ccagtggctg
     8281 aaaggaatat tgacgttaag cgctaacatc tgatcacgtc ctttttgcca ggcgtttttc
     8341 gtcaggctat agctgagcgt ccagttgata tcttcgaacg cagtatttaa tccagcctgg
     8401 aattgctcat cgacattact cgttccccaa taagtttgat ggctaccact caaatacagt
     8461 gttgatgtgc gcccgagttg ctgagtaacg gtgagctgta atttcccgcg tttgttataa
     8521 gcgaggttgt aatagtcggt gaatttcggc ttaacctgaa taactccatc ctgtgtttcg
     8581 atgttgtagc cattcattcg actgtatgtt gtatcagcga aattaaaata tccgctggtc
     8641 gaataacggt aacccactaa ctgaatattc gtgcctgatt cattgagcga tttgttatag
     8701 agaaaacgca ccgattgtcc gtcatgctga ctgtcatcgg gaagtgtgga attagcctgc
     8761 gtcatatcca cagacagagc gcccagtgcc cccatgtttt tcccgatacc gaaattaaaa
     8821 gcacgataac gatccgccag ttgcgttcca ccatatattg tccagccagc cggaaggccg
     8881 tggagtaatg tactctggaa aaagcggggt ttttcctgct gcgcatttcc actacggtat
     8941 tctcctgccg taatggaata acgagtatgc ccttcacgtt gcaaaagcgg gactgacgaa
     9001 tagggtacgg taaaaatctg cgtgctgccg tcagcctctt tgatcgttac ctgcaagtca
     9061 ccactattac ctgcggcata gatatcgttg atggtaaaag gccccggtgg caccgtacta
     9121 ttataaatgt catacccatt ttgtttaata gtgacctgtg cagtaccacg agcaataccg
     9181 tggatcaccg gggcaaatcc tctttggcta tcgggtaaca tattgtcatc tgaggccaat
     9241 tgtgcgccgc gaaagttaat accatcgaaa atatcgccct gagtataacc atcacccagc
     9301 gtcagccggg aacgtaacgg tattatgtct cgctcaagcc aggtattgat atgctgccat
     9361 ttatttttgc tacctgatga tctgtcgcta ctgttataac tccaggtggt attgtcgcgt
     9421 aaacgccacg caccaatatt taacccactc tgtaggttta aatatgcata atggctgtta
     9481 cccccaatcc gattctgtac actatttccg ctgaaattat aattgagcaa tccggcatta
     9541 ataccgggat cccataactc aggaggaata taaccacgcg cgcgattact cataaatgcc
     9601 tgagggatcg tcaggttcag tcgctgctga ccaacatcca gatgcgcagt agcgtcctgg
     9661 accattgtgg ttaatggcac acaggcatca tccgccagca gattcatacc ggcgacagaa
     9721 gccgtattca gccccatact ggcgagttgc gcgcgtgtca ggcagggaac aatcccttgt
     9781 tcactgtcgc ccgtattaaa tgtgacatca cgcgttgcca tataaccatt attcaaatag
     9841 atatcgacgc gatacgtccc tggcggtaat tcttgcccat tttcaaaacg cgataaatcg
     9901 gccacagcct ggggatcatc cgctaaaaaa cgcgggttaa aatagagttc ggcagatgac
     9961 aaaggtgcct gtgcggcaaa agcacaggca acaaagagcc ggacaaaaaa accagccaaa
    10021 cgatgcttac gaatatgcaa gcattgtgtg tttcgctggt aaagtcttaa attcagatat
    10081 gacattattc ctccggttta aagtcacccg gcagtcaatt ctttttattc aggcgaaaaa
    10141 ttccccctgc gttattccat tacgcccgtc attttggggg taagtgcacc ataatcattt
    10201 attgttcggt aagtaatatt gcttcccgca tcagaaggca atttaaccgt gctttcgccc
    10261 attggaggca ccaaggcatt ttcaagaacc cgggttccgg cattcaactc tgttaccgtc
    10321 aggtaatagg gtgtcgggtt aatcagcgtc agagaattcg cgctacgacg aaatcttaat
    10381 ttttctgcgg cctgatcggg tggcaacgct aatttagccg ggcgatagta cagtttaatg
    10441 cggctgataa ttgcgagctg tagcgtattc tcagtcaatt ttgatttatc cattgacgga
    10501 atcgctttaa cattcatcca gaataaactt tcccgatcct gtggcaattg gttatttgtt
    10561 gcatcaagaa tacgcaaggt attctctttt tttcccttca tcgcaaacag aggaggcgtc
    10621 acgataaaac gaccatcctt tacaccatcg gcattttcca cccatgattg aattaaatag
    10681 gtactatttt catcattatt tgtcacggca agttgcactt gtttttgccc tgccggataa
    10741 attacgcgag tcgcacctaa ggccactccc gcttcagcgc gtccggcaac catcattgcc
    10801 ataaacatca ggatacctgc cagcaagcag aatgttattt cctgcgattt ccttacattg
    10861 acgtttttat tactcactgt cctgttcctg ttatcacatt atctgctgaa caattactga
    10921 taggttaaag agaaccaggc ctgggcattg gcgatgccgc cagtaacccg acgcccggta
    10981 gcacgatatt tggcgatgaa atgtagcgaa gtagagcctg agtaaagccg tttccagttt
    11041 gctggaggac gattaatcgg tacgaggttt ccttcatcat caaacaacgc tacgccaata
    11101 ttggtggcta tccctggccc ctctcccacg gaaagcacat ccggattttt accatccgcg
    11161 acaccgtgaa acgccacccc cacacgttca ctcaccaccg tgctacattc ccgtaaatga
    11221 ataacaaaag gcaccggtgc gctatcttcc cctgccgcat gaaaccggtt actgctgatt
    11281 tgccccatat tgaccgtcat ttgtttatca ccggcttcaa tccggcaagt ttccgcaata
    11341 atgacgccct gaaattgcat atttccgccg ggcagcgtgg tattccattt atttccggcc
    11401 agagcaaaca ttggcagcaa cgacgctaac agaaataatc tcttcctttt catcgttatt
    11461 tttatcgcac aagggtgggc atccctgccc gtaatgacgt ccttgaacct gggtaggtta
    11521 ttgatactga accttgaagg ttgcatccgc attagctgca cccggggttg cctcgccgat
    11581 tgcataataa cgcgcctgga acggaatggt gttggtaccg ttattcaggg ttgtttgtgc
    11641 actaaatgtc gcaccgtcca gcgtcagagc attgcctgtt ctgtccagga tctgcacgcc
    11701 aacgtttgtt gcactacctg cagcagaact ctgcagagcc agtacatcag tacgcgtagc
    11761 atcaattgcc gtacctaaga aggcaacagc ggcttttgtg gcaacagtgg tatcgcaatc
    11821 attcaactga atgttaaaac caacggcaga gctggttgct ccagcctgct tcaggctagc
    11881 ggtacgaacc tgtcccaact gaacggtttg atcaacagag cctgcatcaa ctgcgcaagc
    11941 ggcgttaaca acttcccctt taaagtgaac ggtcccacca tttaccgtcg tagtatcggc
    12001 cagagccgct gcggaactga gggacagagc cgacagaaca acgattgcca gagttttaat
    12061 tttcatgctg ctttccttta aaaaaactat ttctaaatcg acatgggcag tcgttctgta
    12121 cactttgttt tgtcaacgag tttgactgcc aacactgcac agttttcccc caaaagatga
    12181 aacatttggg gccattttga ctcatagagg aaagcatcgc gcacaaactt tttcagttta
    12241 tttgttggct taatattcta ttgttatctt tatttataga tttttatatt gcatgaggtg
    12301 gtttttggag agaagaatga ggaagatgcg tcgagccaca gaaacgttag ctttacatat
    12361 agcggaggtg atgtgaaatt aatttacaag agaaataatt tacatatcaa
//
LOCUS       DAZFJF010000126        12328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFJF010000126 DAZFJF010000000
VERSION     DAZFJF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.126"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            128..4090
                     /gene="putA"
                     /locus_tag="ACHKAU_003977"
     CDS             128..4090
                     /gene="putA"
                     /locus_tag="ACHKAU_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5532950.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(4130..4768)
                     /gene="rutR"
                     /locus_tag="ACHKAU_003978"
     CDS             complement(4130..4768)
                     /gene="rutR"
                     /locus_tag="ACHKAU_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO5532951.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            5056..6147
                     /gene="rutA"
                     /locus_tag="ACHKAU_003979"
     CDS             5056..6147
                     /gene="rutA"
                     /locus_tag="ACHKAU_003979"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO5532952.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            6147..6839
                     /gene="rutB"
                     /locus_tag="ACHKAU_003980"
     CDS             6147..6839
                     /gene="rutB"
                     /locus_tag="ACHKAU_003980"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO5532953.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            6851..7237
                     /gene="rutC"
                     /locus_tag="ACHKAU_003981"
     CDS             6851..7237
                     /gene="rutC"
                     /locus_tag="ACHKAU_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO5532954.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            7245..8045
                     /gene="rutD"
                     /locus_tag="ACHKAU_003982"
     CDS             7245..8045
                     /gene="rutD"
                     /locus_tag="ACHKAU_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO5532955.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            8055..8645
                     /gene="rutE"
                     /locus_tag="ACHKAU_003983"
     CDS             8055..8645
                     /gene="rutE"
                     /locus_tag="ACHKAU_003983"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO5532956.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            8656..9150
                     /gene="rutF"
                     /locus_tag="ACHKAU_003984"
     CDS             8656..9150
                     /gene="rutF"
                     /locus_tag="ACHKAU_003984"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO5532957.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            9171..10499
                     /gene="rutG"
                     /locus_tag="ACHKAU_003985"
     CDS             9171..10499
                     /gene="rutG"
                     /locus_tag="ACHKAU_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO5532958.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(10582..10755)
                     /gene="ymdF"
                     /locus_tag="ACHKAU_003986"
     CDS             complement(10582..10755)
                     /gene="ymdF"
                     /locus_tag="ACHKAU_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5532959.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            11716..11979
                     /locus_tag="ACHKAU_003987"
                     /pseudo
     CDS             11716..11979
                     /locus_tag="ACHKAU_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
BASE COUNT         2796 a         3253 c         3328 g         2951 t
ORIGIN      
        1 ttaacatttc attcattttt aagcttgcta cgcatgtcac atttaacatg gttgcacaaa
       61 gttgcaacat catggatatt tcacgataac gttaagttgc acctttcaga acaacaggag
      121 taatggcatg ggaaccacca ccatgggggt taagctggac gacgcgacgc gtgagcgtat
      181 taagtctgcc gcgacacgta tcgatcgcac gccacactgg ttaattaagc aggcgatttt
      241 ttcttatctc gaacaactgg aaaacagcga tactctgccg gagctacctg cgctgctttc
      301 tggcgctgcc aatgagagcg atgaagcacc gactccggca gaggaaccac accaaccatt
      361 cctcgacttt gccgagcaaa ttttgcccct gtcggtttcc cgcgccgcga tcacagcggc
      421 ctatcgccgc ccggaaaccg aagcggtttc tatgctgctg gaacaagccc gcctgccgca
      481 gccagttgct gaacaggcgc acaaactggc gtatcaactg gccgataaac tgcgtaatca
      541 aaaaaacgcc agtggtcgcg caggtatggt tcaggggtta ttgcaggagt tttcgttgtc
      601 atcgcaggaa ggcgtggcgc tgatgtgtct ggcggaagcg ttgttgcgta ttcccgacaa
      661 agccacccgt gatgcgttaa ttcgcgacaa aatcagcaac ggtaactggc aatcacacat
      721 tggtcgtagc ccgtctttgt ttgttaatgc cgccacctgg gggctgctgt ttactggcaa
      781 actggtttcc acccataacg aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa
      841 aagcggtgaa ccgctgatcc gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca
      901 gttcgtcact ggcgaaacca tcgcggaagc gttagccaat gcccgcaagc tggaagagaa
      961 aggtttccgt tactcttacg atatgcttgg cgaagctgca ctgactgccg cagatgcaca
     1021 agcgtatatg gtttcctatc agcaggcgat tcacgccatt ggtaaagcgt ccaatggtcg
     1081 tggcatctat gaaggtccgg gcatttcaat caagctgtcg gcgctgcatc cgcgttacag
     1141 ccgcgcccaa tatgaccggg taatggaaga gctttaccca cgtctgaaat cactcaccct
     1201 gctggcgcgt cagtacgata ttggtatcaa cattgacgcc gaagaggccg atcgtctgga
     1261 gatctccctc gatctgctgg aaaaactctg tttcgagcct gagctggcag gctggaacgg
     1321 tatcggtttt gttattcagg cttatcaaaa acgctgccca ctggtgattg attacctgat
     1381 tgatctcgcc acccgcagcc gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg
     1441 ggatagtgaa atcaagcgtg cgcagatgga cggccttgaa ggctatccgg tttatacccg
     1501 caaggtgtat accgacgttt catatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa
     1561 tctaatctac ccgcagttcg cgacgcacaa cgcccatacg ctggcagcga tttatcaact
     1621 ggcaggtcag aactactatc cgggtcagta cgagttccag tgcctgcatg gtatgggcga
     1681 gccactgtat gagcaggtca ccgggaaagt tgccgacggc aaacttaacc gtccgtgtcg
     1741 tatttatgct ccggtcggta cccatgaaac gctgttggcg tatctggtgc gtcgcctgct
     1801 ggaaaacggt gctaacacct cgtttgttaa ccgtattgcc gacacctctt tgccactgga
     1861 tgaactggtc gccgatccgg tcactgctgt agaaaaactg gcgcaacagg aagggcaaac
     1921 tggattaccg catccgaaaa ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa
     1981 ctcggcaggg ctggatctcg ctaacgaaca ccggctggcc tcgctctctt ctgccctgct
     2041 caatagtgca ctgcaaaaat ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg
     2101 tgagatgtcg cccgttatta accctgcgga accgaaagat attgtgggct ttgtgcgtga
     2161 agccacgccg cgtgaggtag aacaggcgct ggaaagtgcg gttaataacg cgccaatctg
     2221 gtttgccact cctccggttg aacgcgcggc gattttgcat cgcgctgccg tgctaatgga
     2281 aagccagatg cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa
     2341 cgccattgcc gaagtgcgcg aagcggtcga ttttctccac tactatgccg gacaggtgcg
     2401 ggatgatttc gctaacgaaa cccaccgtcc attagggcct gtggtgtgta tcagtccgtg
     2461 gaacttcccg ctggctattt tcaccgggca gattgctgcc gcactggcgg caggtaacag
     2521 cgtgctggca aaaccggcag aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt
     2581 gctggaagcg ggtgtaccgc caggcgtggt gcagttgctg ccaggtcagg gggaaaccgt
     2641 gggcgcgcaa ctgacgggtg atgatcgcgt acgcggggtg atgtttaccg gttcaaccga
     2701 agtcgctacg ttactgcagc gcaatatcgc cagccgcctg gacgctcagg gtcgccctat
     2761 tccgctcatc gctgaaaccg gcggcatgaa cgcgatgatt gtcgattctt cagcactgac
     2821 cgaacaggtc gtcgtggatg tactggcctc ggcgttcgac agtgcgggtc agcgttgttc
     2881 ggcgctgcgc gtgctgtgcc tgcaagatga gattgccgac cacacgttga aaatgctgcg
     2941 cggcgcaatg gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc
     3001 ggtgattgat agcgaagcga aagccaatat tgagcgccat attcagacca tgcgtagcaa
     3061 aggccgtccg gtgttccagg cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag
     3121 cggcaccttt gtcgccccga cgctgatcga actggatgac tttgccgaat tgcaaaaaga
     3181 ggtctttggt ccggtgctgc atgtggtgcg ttacaaccgt aaccagctac cagagctgat
     3241 cgagcagatt aacgcttccg gctatggtct gacgcttggt gtccatacgc gtattgatga
     3301 aaccatcgcc caggtcactg gctcggccca tgttggtaac ctgtatgtta accgtaatat
     3361 ggtgggcgca gtggtgggtg tgcagccatt cggcggcgaa gggttgtccg gtaccgggcc
     3421 gaaagcaggc ggtccgctct atctctaccg tctgctggcg aatcgcccgg aaagtgcgct
     3481 ggcagtgacg ctcgcgcgtc aggatgcaga gtatccggtc gatgcacagt tgaaagccgc
     3541 attgactcag ccgctaaatg cactgcggga atgggcagca aatcgtccag aattgcaggc
     3601 gttatgtacg caatatggcg agctggcgca ggcaggaaca caacgattgc tgccggggcc
     3661 gacgggtgaa cgcaacacct ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga
     3721 tgatgagcag gatgcgctga ctcagctcgc cgccgtgctg gcggtgggca gccaggtact
     3781 gtggccggat gacgcgctgc atcgtcagct agtaaaggca ttgccatcgg cagtcagcga
     3841 acgtattcaa ctggcgaaag cggaaaatat aaccgctcaa ccctttgatg cggtgatctt
     3901 ccacggtgat tcggatcagc ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc
     3961 aattgtttcg gtgcagggtt ttgcccgtgg cgaaagcaat atccttctgg aacggctgta
     4021 tatcgaacgt tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac
     4081 tataggttaa acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc
     4141 gaatcccctc aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatatctcat
     4201 cgcgcaacgt cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag
     4261 tggaagccca aatcatgaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt
     4321 tgacccaacc ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca
     4381 gttcatccat taacagcggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct
     4441 gcggataatc gcgggagact tccagcttca gacggatgta ctcttttatc gccgccagcg
     4501 gagcgaaatc ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca
     4561 gcacggcaat atacagcgcc tcttttgacg gaaagtaata cagcagattg gtttttgaaa
     4621 cacccgccaa ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag
     4681 tgtccagtgc tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac
     4741 ccgttgtttt cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggcaacagga
     4801 tagcaaaacc cccgcccggc ctcgaccttc tgcactctca tcgcgcacag atgcatgttt
     4861 tatgtgcaac tgttttgacc gtttagtcca ctttttacca gatattttag acaacacaat
     4921 gttaacacat taataacatt cactttttaa aaactggcat ccgctttgca aacaagccat
     4981 tacgcggctg aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac
     5041 gaagagaggt tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc
     5101 atttcgaccc acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa
     5161 aaagcggagc actaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc
     5221 ggcaaaactg agttctggga tcacaacctt gagtcgttca ccttgatggc ggggctggcg
     5281 gccgtcacct cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca
     5341 atcgtcgccc gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac
     5401 ctcgtgactg gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac
     5461 tatttctccc gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg
     5521 ggcacgggga aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt
     5581 ccgcaaccga gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg
     5641 ttctccgccc agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacgcccacg
     5701 gctttcgccc cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg gcgcgacgtt
     5761 ggttcttatg tgttgtttat ggtgattgcc gatgaaaccg acgatgccgc tcgcgccaaa
     5821 tgggaacact acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt
     5881 cagaaagata cccgctccgg tactgatacc aacgtccgtc agatggccga tcccacttcg
     5941 gcggtaaaca tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta
     6001 gatgaagtcg caagcgtgcc tggtgccgaa ggcgtgctgt tgaccttcga tgattttctg
     6061 tcgggaatcg aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatcta
     6121 cctgcgctga ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta
     6181 ccttcgatcc gcagcaaagt gcgctgatcg tggtggatat gcaaaacgct tatgccacgc
     6241 caggcggcta cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca
     6301 acattcaaac cgccgtgacc gcagcgcgag cggcagggat gctgatcatc tggtttcaaa
     6361 atggctggga tgaacagtat gtcgaagctg gcggccccgg ctcaccgaat tttcataaat
     6421 cgaacgccct gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag
     6481 gctcctggga ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc
     6541 cgaagccgcg ctacagcggt ttcttcaata caccgctgga cagcattttg cgcagccgtg
     6601 gaatacgcca tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac
     6661 gcgatggctt ttttctggag tatttcggcg tggtgctgga agacgcaaca caccaggcgg
     6721 ggccggaatt tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca
     6781 gcgacgtcga aacgttctgc gacgcgcttt ctcccacatc ctttgctcgt atcgcttaag
     6841 gagtttaacg atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc
     6901 cttcgttccc ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga
     6961 tcaacataat aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga
     7021 aattatccgc aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag
     7081 catctttatt accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt
     7141 tccgggtgat aaaccggcgc gattctgcat tcagtgcgga ctggtaaaac ctgacgcgct
     7201 ggtggaaatc gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct
     7261 cacctccccc ttatgctgat acgcccgtag tggtgttgat ttcgggtctt ggcggtagcg
     7321 gcagttactg gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg
     7381 accagcgcgg caccggcaat aatcccgaca cgctggcaga agattacagt atcgcccaga
     7441 tggcagcgga actgcatcag gcgctggtag ccgcagggat tgagcattac gcggtggtcg
     7501 gccatgcgct cggtgcgctg gtgggaatgc agctggcgct ggattatccc gcgtcggtaa
     7561 ctgtgctgat cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc
     7621 aggttcgcga acgattactg tatagcggcg gcgcgcaggc atgggtggaa gcgcagccgt
     7681 tgttcctcta tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg
     7741 cgctggcact ggcgcatttt cagggcaaaa ataatttact gcgtcgactt aacgccctca
     7801 aacgcgctga ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg
     7861 ccagtgatga tctgctggtg ccatcagcat gttccagtga gcttcatgcc gccctgcccg
     7921 atagccagaa aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa
     7981 cgtttaatgc tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc
     8041 tgtaaggaat tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ttgttcaccg
     8101 atgcccgcac tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacgggaga
     8161 tttatgccct gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt
     8221 ttatccgcac ggcagaagga aaagaacgtc tgcgcccggc actttccagc ggcaatctgc
     8281 aaaaaaccct gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac
     8341 ggttaccgca actgtttcct cacggcgatg cccgcagttg gtttacctcc agcccacaac
     8401 ttgccgaaga aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgcct
     8461 gccgtgcgct gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg
     8521 acgccttttt tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg
     8581 atagcagcaa actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt
     8641 tgtaaggagc aaaccatgaa cattgtcgat caacaaacct ttcgcgatgc gatgtcctgt
     8701 atgggcgcgg cagtcaatat catcaccacg gatggtccag ccgggcgcgc cgggttcacc
     8761 gccagcgcgg tctgcagcgt gaccgacacg ccgcctacat tactggtgtg cctgaatcgt
     8821 ggggcgtccg tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc
     8881 gccgggcagg agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt
     8941 gccgccgccc gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt
     9001 tcgtttgact gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc
     9061 gccatcgaag cgattcatcg tcacgccaca ccctacgggc tggtgtggtt cgatcgcagt
     9121 tatcacgcgc tgatgcgccc tgcttgttaa cctcctaaag gagacagctg atggcaatgt
     9181 tcggttttcc tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgt
     9241 atgaacgatt gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt
     9301 ttggcgcgac ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa
     9361 tgtcggggat cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc
     9421 tcggttccag cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg
     9481 gcattaaccc gaatatcagc attgcgctgg gcgggattat cgcctgtggg ctggtttata
     9541 ccgtcattgg cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc
     9601 cggtggtgac gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga
     9661 aaagtgtatc ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg
     9721 ggctggtggc ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggtttaa
     9781 ttgtcgcctg tctgctgtat ggcgtgatga ccaacctttt agggctggga aaagccgtcg
     9841 attttactct cgtcagccat gctgcctggt tcggtttgcc acatttttcc actcctgctt
     9901 ttaatagtca ggcaatgatg ctgattgcgc ccgtggcggt gattctggtg gcggaaaact
     9961 taggtcatct caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc
    10021 gggcattcgt cggtgatggg ctggcgacga tgctttctgg ttctgtcggt ggcagcggcg
    10081 taaccaccta cgccgagaat atcggtgtaa tggccgtgac aaaagtctac tcgacgctgg
    10141 tgtttgtcgc tgctgccgtc atcgccatgc tgttgggctt ttcaccgaaa tttggcgcgc
    10201 tgattcatac cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga
    10261 ttgccgtcgc aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacggta
    10321 atttgattat ggtcgcagta accttagtgc tgggcgcggg tgattttgct ctcacgctgg
    10381 gcggttttac gttgggaggg attggtacag caacctttgg cgcgatttta ctcaatgcgt
    10441 tgttaagcag aaagttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa
    10501 cagaaaaccg ccgggattac cggcggtgat gactttttga cgagcctaag aaaaaaatgc
    10561 attcgtcatt gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt
    10621 tttacctgct tcagatgcgc gctgcgggtc attcttgaaa ttacccccgc tgtgctgtcc
    10681 acctttttta cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc
    10741 tcgatggttt gccattactg acctccgtct gggtatagac atcatattgc atagaattaa
    10801 cgcggcatat ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc
    10861 gggcagaccc gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct
    10921 caaataatta tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt
    10981 cattttgcga caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga
    11041 aatttgcact ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa
    11101 tagagataac acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg
    11161 tacggacata ttgaaaccat gaagcattaa aaacacagaa ttaccgaaga tcgtccaaga
    11221 gcatatactt ttcaatatgt tagacctcat tttgaccata aagaaatcga ttttacaccg
    11281 ttctttatac gttctttttt gccccaaata tgccccaaac aaccacactt tgccccaata
    11341 gcgccccaaa gatttaaggg gatttaagta tatatttcag ataattaacg caagtttacg
    11401 atgtgaattt acgttaattg tctgggtggg gtatataccg tcactcgttc atcaatattc
    11461 tcatagtttg acaaatccgc cagagctatc gcatactgat cgcactgaac aaataagcgg
    11521 tctccgcacc cgatagcttt gcggcttttt tatgcctgca atttggcata gttacatccg
    11581 tacaaaggtc gggtggagag gcgtaataca acacccgcaa ggggaatatg cccagagctt
    11641 cttatttggc tcagttgaca cccgaccgcc agctactaac tggcggttat aaactaaaca
    11701 aataaggagg tcatcatgac cagtcaactc atccccgtat tcaacggcac tatcgacaac
    11761 gaaatagccc tactctgcaa tgcccgcgat ctgcatgctt ttttaggcgt gaaaaaggtg
    11821 tttgcggcat ggattacaaa tcgcatttca gaatacgaat tcattgaaaa tcaagactat
    11881 attttgcttt ccaatttggg aaagcaaaca tctggtagag gcggtcacaa ccgcaaggac
    11941 taccacctca cactggacac agccaaagaa ctggcgatac cattcccgcc aggcccactt
    12001 acaacagtaa aattcggggc tctgccttta catacaatac aatgttgcac gattgcccat
    12061 catcaactcc gttaatatct atgagtagta accctatgta actgtcattt accccaacaa
    12121 gactaaattt tagaaaaaac aactgccggg acaactttcg ccccctgtaa ccccaaataa
    12181 gatgtcagat ttatcggcat taatttatct ccctgttgaa ttagcacctc tatcatctac
    12241 ttaacaaata tcccacggac attgcaacac aaaaaccgga gccggactcc ggttttgtga
    12301 agctgtcggg ttacttcatc ccgccaat
//
LOCUS       DAZFJF010000127        12165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFJF010000127 DAZFJF010000000
VERSION     DAZFJF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.127"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..338
                     /locus_tag="ACHKAU_003988"
     CDS             42..338
                     /locus_tag="ACHKAU_003988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5532960.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(496..1143)
                     /gene="ychE"
                     /locus_tag="ACHKAU_003989"
     CDS             complement(496..1143)
                     /gene="ychE"
                     /locus_tag="ACHKAU_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO5532961.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1174..1227)
                     /gene="ychE"
                     /locus_tag="ACHKAU_003990"
     CDS             complement(1174..1227)
                     /gene="ychE"
                     /locus_tag="ACHKAU_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO5532962.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1620..4295
                     /gene="adhE"
                     /locus_tag="ACHKAU_003991"
                     /gene_synonym="adhC"
     CDS             1620..4295
                     /gene="adhE"
                     /locus_tag="ACHKAU_003991"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO5532963.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4419..5315
                     /locus_tag="ACHKAU_003992"
                     /pseudo
     CDS             4419..5315
                     /locus_tag="ACHKAU_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5597..6214)
                     /gene="tdk"
                     /locus_tag="ACHKAU_003993"
     CDS             complement(5597..6214)
                     /gene="tdk"
                     /locus_tag="ACHKAU_003993"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO5532964.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6819..7232
                     /gene="hns"
                     /locus_tag="ACHKAU_003994"
     CDS             6819..7232
                     /gene="hns"
                     /locus_tag="ACHKAU_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO5532965.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7376..8284)
                     /gene="galU"
                     /locus_tag="ACHKAU_003995"
     CDS             complement(7376..8284)
                     /gene="galU"
                     /locus_tag="ACHKAU_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO5532966.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8486..9499)
                     /gene="rssB"
                     /locus_tag="ACHKAU_003996"
     CDS             complement(8486..9499)
                     /gene="rssB"
                     /locus_tag="ACHKAU_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO5532967.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9591..10496)
                     /gene="rssA"
                     /locus_tag="ACHKAU_003997"
     CDS             complement(9591..10496)
                     /gene="rssA"
                     /locus_tag="ACHKAU_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO5532968.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10609..11067
                     /gene="ychJ"
                     /locus_tag="ACHKAU_003998"
     CDS             10609..11067
                     /gene="ychJ"
                     /locus_tag="ACHKAU_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO5532969.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            11117..11959
                     /gene="purU"
                     /locus_tag="ACHKAU_003999"
     CDS             11117..11959
                     /gene="purU"
                     /locus_tag="ACHKAU_003999"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO5532970.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         3305 a         3002 c         2741 g         3117 t
ORIGIN      
        1 accaataaat aatcgtctgg cggttgcccg gaaagtacca aatgcggttc ttactcgcca
       61 atacattttg caaaattgtt aagcaattct cttttattga ccgtatcaga atctatagct
      121 tgcatccatg ccttaacaaa catctttcaa ttcaatcaaa tagagattgg ctaagcatgt
      181 ctatcttttc tgattacagt tctagtagtg aaatgcacaa taatttaaca attgattatt
      241 atttagcact ttcctctacc aaaggcagtg gaattactaa tatcatttca attattcttc
      301 agcaagccca ggattatgat gtggcgaaaa taacatgatg gtgaggagaa gcagttaagg
      361 aaatccttat aactaactgt aatttgagaa gaatattttt acctattcct tttgcttttg
      421 ataactgcgc gggaacaaag taaaaataat gacgaagtaa acggagcatg gcagctccgt
      481 ttcatttgaa aggaattaat taagcaggcc ggggaaaatc cccttaatac cagtaacgat
      541 aaattcaatc cccaatgcca tcagcaatag ccccataata cgcgtaatca cgttgatgcc
      601 ggtctggcgt aaaacccgta ccagccacgg tgccatgcgg aacaatcccc agcaacataa
      661 agcgaacaat gcaatagcca caaagaaacc aaacagatag ctaatgctgt gataacgcgt
      721 accccagacg atggtagaac tgatcgcccc tggccccgcc atcaacggca acgccaatgg
      781 caccacacca atgctttcac gtaccgcggt ttctgatttt tcttgcttgt tctgtttatc
      841 ctcgccaagc ttgccgctga tcatcgacat cgctatcgtc accaccagga tgcccccggc
      901 gatacggaac gaatcaattg atataccaaa aagttgcaga atcgtgtcgc cgagaaaaag
      961 cgagatccac aagataatgg ccacagacag gttggctgta aggttagttt tgtttcgcgc
     1021 tgccgctgtc tgataactgg tcatgctgat aaagacggga ataatcccta ccgggttgac
     1081 cagcgcaaat aacccgatga aaaatttgaa gtaaacggga aaatcaaaaa aggtctgaat
     1141 cacggttagc tccgaagcaa aagccggata atgttagcca taaataaggt tgaaaagacg
     1201 cgctgacaat acgccttttg acagcatttt tcacctccta actacttaaa attgctatca
     1261 ttcgttattg ttatctagtt gtgcaaaaca tgctaatgta gccaccaaat catactacaa
     1321 tttattaact gttagctata atggcgaaaa gcgatgctga aaggtgtcag ctttgcaaaa
     1381 atttgatttg gatcacgtaa tcggtaccca gaagtgagta atcttgctta cgccacctgg
     1441 aagtgacgca ttagagataa taactctaat gtttaaactc ttttagtaaa tcacagtgag
     1501 tgtgagcgcg agtaagcttt tgattttcat aggttaagca aatcatcacc gcactgacta
     1561 tactctcgta ttcgagcaga tgatttacta aaaaagttta acattatcag gagagcatta
     1621 tggctgttac taatgtcgct gaacttaacg cactcgtaga gcgtgtaaaa aaagcccagc
     1681 gtgaatatgc cagtttcact caagagcaag tagacaaaat cttccgcgcc gccgctctgg
     1741 ctgctgcaga tgctcgaatc ccactcgcga aaatggccgt tgccgaatcc ggcatgggta
     1801 tcgtcgaaga taaagtgatc aaaaaccact ttgcttctga atatatctac aacgcctata
     1861 aagatgaaaa aacctgtggt gttctgtctg aagacgacac ttttggtacc atcactatcg
     1921 ctgaaccaat cggtattatt tgcggtatcg ttccgaccac taacccgact tcaactgcta
     1981 tcttcaaatc gctgatcagt ctgaagaccc gtaacgccat tatcttctcc ccgcacccgc
     2041 gtgcaaaaga tgccaccaac aaagcggctg atatcgttct gcaggctgct atcgctgccg
     2101 gtgctccgaa agatctgatc ggctggatcg atcaaccttc tgttgaactg tctaacgcac
     2161 tgatgcacca cccagacatc aacctgatcc tcgcgactgg tggtccgggc atggttaaag
     2221 ccgcatacag ctccggtaaa ccagctatcg gtgtaggcgc gggcaacact ccagttgtta
     2281 tcgatgaaac tgctgatatc aaacgtgcag ttgcatctgt actgatgtcc aaaaccttcg
     2341 acaacggcgt aatctgtgct tctgaacagt ctgttgttgt tgttgactct gtttatgacg
     2401 ctgtacgtga acgttttgca acccacggcg gctatctgtt gcagggtaaa gagctgaaag
     2461 ctgttcagga tgttatcctg aaaaacggtg cgctgaacgc ggctatcgtt ggtcagccag
     2521 cctataaaat tgctgaactg gcaggcttct ctgtaccaga aaacaccaag attctgatcg
     2581 gtgaagtgac cgttgttgat gaaagcgaac cgttcgcaca tgaaaaactg tccccgactc
     2641 tggcaatgta ccgcgctaaa gatttcgaag acgcggtaga aaaagcagag aaactggttg
     2701 ctatgggcgg tatcggtcat acctcttgcc tgtacactga ccaggataac caaccggctc
     2761 gcgtttctta cttcggtcag aaaatgaaaa cggctcgtat cctgattaac accccagcgt
     2821 ctcagggtgg tatcggtgac ctgtataact tcaaactcgc accttccctg actctgggtt
     2881 gtggttcttg gggtggtaac tccatctctg aaaacgttgg tccgaaacac ctgatcaaca
     2941 agaaaaccgt tgctaagcga gctgaaaaca tgttgtggca caaacttccg aaatctatct
     3001 acttccgccg tggctccctg ccaatcgcgc tggatgaagt gattactgat ggccacaaac
     3061 gtgcgctcat cgtgactgac cgcttcctgt tcaacaatgg ttatgctgat cagatcactt
     3121 ccgtactgaa agcagcaggc gttgaaactg aagtcttctt cgaagtagaa gcggacccga
     3181 ccctgagcat cgttcgtaaa ggtgcagaac tggcaaactc cttcaaacca gacgtgatta
     3241 tcgcgctggg tggtggttcc ccgatggacg ccgcgaagat catgtgggtt atgtacgaac
     3301 atccggaaac tcacttcgaa gagctggcgc tgcgctttat ggatatccgt aaacgtatct
     3361 acaagttccc gaaaatgggc gtgaaagcga aaatgatcgc tgtcaccacc acttctggta
     3421 caggttctga agtcactccg tttgcggttg taactgacga cgctactggt cagaaatatc
     3481 cgctggcaga ctatgcgctg actccggata tggcgattgt cgacgccaac ctggttatgg
     3541 acatgccgaa gtccctgtgt gctttcggtg gtctggacgc agtaactcac gccatggaag
     3601 cttatgtttc tgtactggca tctgagttct ctgatggtca ggctctgcag gcactgaaac
     3661 tgctgaaaga atatctgcca gcgtcctacc acgaagggtc taaaaatccg gtagcgcgtg
     3721 aacgtgttca cagtgcagcg actatcgcgg gtatcgcgtt tgcgaacgcc ttcctgggtg
     3781 tatgtcactc aatggcgcac aaactgggtt cccagttcca tattccgcac ggtctggcaa
     3841 acgccctgct gatttgtaac gttattcgct acaatgcgaa cgacaacccg accaagcaga
     3901 ctgcattcag ccagtatgac cgtccgcagg ctcgccgtcg ttatgctgaa attgccgacc
     3961 acttgggtct gagcgcaccg ggcgaccgta ctgctgctaa gatcgagaaa ctgctggcat
     4021 ggctggaaac gctgaaggct gaactgggta ttccgaaatc tatccgtgaa gctggcgttc
     4081 aggaagcaga cttcctggcg aacgtggata aactgtctga agatgcattc gatgaccagt
     4141 gcaccggcgc taacccgcgt tacccgctga tctccgagct gaaacagatt ctgctggata
     4201 cctactacgg tcgtgattat gtagaaggtg aaactgcagc gaagaaagaa gctgctccgg
     4261 ctaaagctga gaaaaaagcg aaaaaatccg cttaatcagt agcgctgtct ggcaacataa
     4321 acggcccctt ctgggcaatg ccgatcagtt aaggattagt tgaccgatcc ttaaactgag
     4381 gcactataac ggcttccaca acagggagcc gttttcttat gccacttctt aatgatctgc
     4441 tcgatttcag tgaccatccg cttatgcctc cgccctctgc acaactattt gcagaacacc
     4501 ttcccaccga gtggatacaa cactgcctga cgctttctgc tcatgcgacc gttcgccgcc
     4561 gtcgtttacc gggggacatg gttatctgga tggtggtgca atgagccaat taccgatgtt
     4621 gttcgccgtc tgaacctgag cgcggatggc gaagcgggga tgaacctgct ggcccgcagc
     4681 gctgtcaccc aggcgcgtca gcgcgtgggg gccgccccag tggaatggct cttccgccag
     4741 accgcacaga caggggcgcg gaacgttacc tgaaggatga ctggcacggc ctgcaacttt
     4801 ttgccattga tggcgcacag ttcaggacac ctgataaacc cgagctgcgt gaatattatg
     4861 ggtctgccaa cacatccact aagcggcaga acgcctaccc ggttatgcgt ctggtggcct
     4921 taatgaacct gggaagtcac attctgctga atgccgtgac cgcaccttac cggcagagcg
     4981 aaactgtgct ggcccactcc atgctcgcca ccatcccgga taactctatt acgttgtttg
     5041 ataaactttt ttacagcgaa gacctgctgc tgacgctgaa ccagaagggt tgtaaccgcc
     5101 actggctact gcctgcgtgg aagaatatcg catcagaaat gatagagctg ggaaacacgg
     5161 cgtcaacagg gaccatcccg aaacgactgg agcatcttcg gggtgctctg gaagtggtgt
     5221 tcataacaaa acgcccccga ccatcaaggc cgaggtcggt taagatatca aaaacccgtt
     5281 atcctgtgaa acatagtgcc gctcctctta agtgaacggc attgcccttc tggggccgta
     5341 ttttttgttt acccaaagca acttttccat aaaccgacag cattagcctt catcatattt
     5401 gcgacgatgt ataacgccta aacacaggga tattgtactt tacaggtcac aagtcaacgt
     5461 cggtgcttaa gagccctgtg aggcgtatag cggcgttaaa aaactgccga gaagggtata
     5521 tagcccggaa gaagtgcgta aaacgaactg acaggataaa agtgccctgc tcaccctgtc
     5581 agtaaagaaa ttcttattaa tcgtggcgat gcctttcctg aatagccgtt aatgagccga
     5641 cttgtaacgc ctctttatag tgtttacggc atacagaaac gtatcgttca ttaccaccaa
     5701 ttaccacctg ctcaccttcg ttataaggtc tgcctgcttg atcaagacgc agcaccatgc
     5761 ttgctttacg gccacaaaaa cagatggttt ttaattcaac cagtttgtcg gaccatgcca
     5821 gtaagtattg gctgccaata aataattcac ctcgaaaatc ggtacgtaaa ccataacaaa
     5881 gtacgggtat atcgagttga tcgacaacct ccgataattc atatacttgt tgtctggtta
     5941 aaaactggca ttcatcaacc agtacgcaat gaattgcctg ctgttcatgt tccgcacgaa
     6001 tctcatcaaa taatgatgaa ttttggttaa ataattttgc aggcgatgac aaacctatac
     6061 gcgaactgac tttcccggca ccaaagcgat catcaatttc tgccgtatat acgacagtgc
     6121 gcatgccgcg ttcctggtaa ttgtatgaag attgcaacaa tgctgtagac ttacccgcat
     6181 tcattgcaga atagtagaaa tatagctgtg ccatcagcca caggccctca atgatatgaa
     6241 ataaagttgg ctggagttta tcataattcg tcagtttttc agctcaacag tatgcacaga
     6301 agtgggtata tgcgttctcc cttacgaagc cttgcataat ccttctgagc tatcattaca
     6361 actgcctcgc ttgttataag cgggtaaatg actgctggta actattcaca atctttaacc
     6421 tgttgcgcaa gtaatagccc tctgttgacc tccaggagat agtgcaatac taagtccatg
     6481 ctcttattgc gactgttcta cttttcatca ttcgcttaat agggaattct cgtaaacaca
     6541 actaatacag aagactgaaa ggtcgtcagc ctacgataat ctccccataa aatgtgacat
     6601 gaatcaggaa gttttaacct cacgtgctgc gaaatcatcg gtgtaaatag ggctatatgc
     6661 cgcgtctttt ctggctaatt ttatgaaaag atatttattg gcggcacaaa ataaagaaca
     6721 attttgaatt ccttacattc ctggctattg cacaactgaa tttaaggctc tattattacc
     6781 tcaacaaacc accccaatat aagtttgaga ttactacaat gagcgaagca cttaaaattc
     6841 tgaacaacat ccgtactctt cgtgcgcagg caagagaatg tacacttgaa acgctggaag
     6901 aaatgctgga aaaattagaa gttgttgtta acgaacgtcg cgaagaagaa agcgcggctg
     6961 ctgctgaagt tgaagagcgc actcgtaaac tgcagcaata tcgcgaaatg ctgatcgctg
     7021 acggtattga cccgaacgaa ctgctgaata gccttgcttc cgttaaatct ggcaccaaag
     7081 ctaaacgtgc tcagcgtccg gcaaaatata gctacgttga cgaaaacggc gaaactaaaa
     7141 cctggactgg ccaaggccgt actccagctg taatcaaaaa agcaatggat gagcaaggta
     7201 aatccctcga cgatttcctg atcaagcaat aatcttttgt agattgcact tgcttaaaat
     7261 cccgccagcg gcgggatttt ttattgtccg gtttaagaca atttaataag aataagctat
     7321 aaaaaaacgg cgtcgattgc tcaacgccgt ttcgtggata acaccgatac ggatgttact
     7381 tcttaatgcc catctcttct tcaagccagg ctttaaattc cgtgccaagg gtgttatgac
     7441 gaataccgta ttcaacgaag gcctgcatgt aacctaattt attaccgcag tcatggctct
     7501 tccctttcat atgataggct tccaccgttt ctttttcaat cagcatatca attgcgtcgg
     7561 tgagctgaat ttcatcacca gctcccggag gggtttttgc cagcaacggc caaatatccg
     7621 cgctaagtac gtaacgaccc acaatagcga gattagacgg cgcaacatcc gctttcggtt
     7681 tttctaccac accaaccatc ggtacgcttt cacccggcgc taattcaacg cctttgcaat
     7741 ccacaacgcc atatgcggtc acatcagcaa ccggttcaac catgatctgg ctatgacccg
     7801 tttcatcaaa gcggcggatc atctctgcca ggttatcctg tgacaaatcg gattcatatt
     7861 catccagaat aacatcaggc aaaataacag ctaccggttc atcacccact accgggtgag
     7921 cacacaatac cgcgtgtccc aggcctttcg ccagaccctg acgaacttgc ataatagtca
     7981 cgtgcggtgg acaaatagac tgcacttcat caagcagttg acgttttaca cgtttttcca
     8041 gcattgcttc cagttcaaaa ctggtatcaa agtggttttc aatagagttt ttagatgagt
     8101 gtgtaaccag cacaatttca gtaatgccag ccgcaataca ttcattcacg acgtattgaa
     8161 ttaatggctt atcgacaagt ggcagcatct ctttcgggat ggctttcgtc gccggcaaca
     8221 tcctggttcc taatcccgca acggggataa cggctttttt gactttcgta ttaatggcag
     8281 ccatttaaat tctcctggac tgttcgtgtt ttgaacgtgt tcatatttct gtatcgcatc
     8341 ccagtatatc agcaccccga aggggctaag ttctgaaaag gaggcgagtt agcataatta
     8401 agacaattac ttatacgaat ggcgacgata gtaccaccag ccgacattag caggtaatgc
     8461 aaatttagcc cgcgttatcg tttgctcatt ctgcagacaa catcaagcgc agtcgaccac
     8521 cggttcccca tatttggcat tgccaggcat cgcatcgctg gctcaattga ttcaaataag
     8581 cgttacctaa agtgcctaac ggaacaccat tactgatttg cacctggtgt tcgccggtat
     8641 ttaacgtcgc attcagaccc gcagaaacca gaatgagatt tttcagttcg cgatgataat
     8701 agccaactaa tagcggaaac tgccccggca gattggcctg acgaagtaaa tggtttacct
     8761 gcttcaataa cgcgcccaac tctggcaacc gttgattttg gtgtgcaagc tgttcctgta
     8821 ataatccgtt aaacaatgcg cgtaataaca aggcagcaag tacgccatta tgtccagctc
     8881 gggtgacatc aaggcaataa aatgccagat cgttttccga aagtgcggca atatcaagca
     8941 ccaggccggg tttgtccgcg gcaaccaatt gacgataatt aacccggcaa tgggaaatca
     9001 cctgctgaac cggcggttgt agttcctgta ataatttcgc cgctgcggca gggttatcaa
     9061 ccattgcatc ccagtcgcga aaaagccttt cctcttcctc aacgcgcgaa ttaaacatgc
     9121 tgggatagag acaggcaaaa accatctcgc gcaagcgatt caaatcttta actggtttca
     9181 gcaaaacatc ttcaacgccc agacgtaacg ctttggcaat atctgccata ttttcggtgg
     9241 cagatatcac cagaaccggt gtctggtcac ctctgttgcg tatatgttcc agcagtttaa
     9301 gcccgttcat tcgtggcatc gcgatatcac atatcatcag gtctggagtg aaacctccca
     9361 gcaactcaag ggcatccacc ccatcagccg ccagtaccgt tgtcgctccc aatgaggaaa
     9421 accacgaatc cagaagcgag cgaaataccg gctcatcttc aacaatgaga atctgttttc
     9481 cgaccaatgg ctgcgtcatg ttctctcccc tgactggctt tactcaatag tggcatgcta
     9541 ttgccacctg cgcctgtcag aattaactta agtgtagata aaaattctgg tcaaatgttg
     9601 gtgcgtacca acggcaaaag ttcgtccatt ttcttttcca ctgccagctg tccggccgca
     9661 atggcagcgt gcgcgcgatg gaaatcaagc gtagatattt gcgggcaaac aggttgaatc
     9721 agaatatcgg gcggatcacc tgccatgcgg ttccttttaa ggcggttctc cagcacctgg
     9781 atagaagtgg tcataatctc tgttgccgtt ggcgctgtca ccgcccgacg tgtcgttatg
     9841 ctgcctaacc tttctttcag acgcgcatgc cacggcagag aatcaccatt ctcgctattt
     9901 tcttcactga cattaaagga gagcaaatct tgttgcatca aatgagcatc gtgctgcagg
     9961 tcaaccgcta tcacaatatc agcccccaat gcacgcgtga gggaaatagg aattgggtta
    10021 acgactgctc catcaaccag ccagtagccg ttatgtgcaa caggtgccat gagtcctgga
    10081 atactgcatg atgcgcgaat agcaagatgg agatcgcctt cagtaaacca taattcacgt
    10141 cccgtactta aattggtggc aacagccgca aagcgacggg aacaattttc gatctctgtt
    10201 tccggcatta tttcgcggta ttgattgaag acacgctcgc cgcgcagtaa cccaccgcgc
    10261 tgccaggaga gatccatcag gcgtaaaaca tcccaataac tgaaagaggt cacccaatct
    10321 tccagcgcag ataatcgatc gcatgcatag gcagcgccca ccagcgaacc aattgaacat
    10381 cctgcaacga tatcaatttc aatacccact ttttttagcg cattaataac gccaatatgc
    10441 gaccaacctc tcgccgcgcc agatcccagc gccagcccta tttttatctt tctcattatt
    10501 cccgcaagat tcccctggaa tgcaatcgta gccacatcgc tactgctcgg ttaacatagt
    10561 gctactcagg cggttaatac gccgttattg tttccaggga gattatttgt gtctcagctt
    10621 tgtccctgtg gtagtgctgt cgagtatagc ctatgttgcc acccttatgt gtctggtgaa
    10681 aaggttgcac ctgatccaga acatctcatg cgttcgcgtt actgcgcttt tgtgatgcaa
    10741 gacgcagatt atttaataaa gacctggcat ccctcttgtg gagcagcggc gttacgtgcc
    10801 gaattgatgg ccggatttgc gcatactgaa tggttgggat tgaccgtatt tgaacattgt
    10861 tggcaggacg ccgacaatat cggatttgtc agttttgttg ctcgatttac cgaaggtggt
    10921 aaaacaggtg caataattga acgttcgcga tttttgaaag aaaacggtca atggtactat
    10981 attgacggta cacgtccgca gtttggtcgc aacgatccct gcccttgtgg ttcaggtaaa
    11041 aaatttaaaa agtgctgcgg ccaataatgg ttgacggtac ggtttagcaa acactctcaa
    11101 caaggttttt ccagcaatgc attcactcca acgtaaagtt ctgcgtacta tttgtccgga
    11161 ccaaaaaggt ctgatcgcac gtattaccaa tatttgctac aagcacgagt taaatatcgt
    11221 acagaacaat gaatttgttg atcaccgtac cgggcgcttt tttatgcgca cggaactgga
    11281 agggattttt aatgattcca ccctgctggc ggatctcgat agcgcattgc cagaaggctc
    11341 cgtgcgtgag ctgaatcctg ccggtcgtcg ccggatagtg attctggtca ctaaagaagc
    11401 gcattgcctt ggcgatttgt tgatgaaagc caattacggc ggcctggatg tcgaaatcgc
    11461 ggcagttatt ggtaaccacg atactttacg ttctctggtt gagcgttttg atattccgtt
    11521 tgagctggta agccatgaag ggttaagccg caacgagcac gatcaaaaga tggcggacgc
    11581 cattgatgct tatcaatctg actatgtggt gctggcgaag tatatgcggg tattaacgcc
    11641 ggaatttgtg gcacgcttcc cgaataaaat catcaatatt caccattcat tcctgcctgc
    11701 gtttatcggc gcacgtcctt atcaccaggc ctatgaacgt ggtgtgaaga ttattggcgc
    11761 aaccgctcac tatgtgaatg acaatctgga cgaaggccca atcatcatgc aggacgttat
    11821 tcatgtcgat catacctaca cagctgaaga tatgatgcgc gcaggtcgtg acgtcgagaa
    11881 aaacgtctta agtcgtgcgc tatacaaagt actggcacag cgcgtctttg tttacggtaa
    11941 tcggacgatt attctttaat cgccagcaaa aataactggt tacctttaat ccgttacgga
    12001 tgaaaattac gcaaccagtt catttttctc aacgtaacac tttacagcgg cgcgtcattt
    12061 gatatgatgc gccccgcttc ccgataaggg agcaggccag taaaaagcat taccccgtgg
    12121 tggggttccc gagcggccaa agggagcaga ctgtaaatct gccgt
//
LOCUS       DAZFJF010000128        11895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFJF010000128 DAZFJF010000000
VERSION     DAZFJF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.128"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..452
                     /locus_tag="ACHKAU_004000"
     CDS             <1..452
                     /locus_tag="ACHKAU_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO5532971.1"
                     /translation="ITHIDTNNTSDIGTLSPIIKQSKNLNFLNVSGKNNCPTMQLMLF
                     NLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTEEVVN
                     QRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            776..2164
                     /gene="narU"
                     /locus_tag="ACHKAU_004001"
     CDS             776..2164
                     /gene="narU"
                     /locus_tag="ACHKAU_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO5532972.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2246..5986
                     /gene="narZ"
                     /locus_tag="ACHKAU_004002"
     CDS             2246..5986
                     /gene="narZ"
                     /locus_tag="ACHKAU_004002"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO5532973.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5983..7527
                     /gene="narH"
                     /locus_tag="ACHKAU_004003"
     CDS             5983..7527
                     /gene="narH"
                     /locus_tag="ACHKAU_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5532974.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7527..8222
                     /gene="narW"
                     /locus_tag="ACHKAU_004004"
     CDS             7527..8222
                     /gene="narW"
                     /locus_tag="ACHKAU_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5532975.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8219..8899
                     /gene="narI"
                     /locus_tag="ACHKAU_004005"
     CDS             8219..8899
                     /gene="narI"
                     /locus_tag="ACHKAU_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5532976.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8978..9871
                     /locus_tag="ACHKAU_004006"
     CDS             8978..9871
                     /locus_tag="ACHKAU_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO5532977.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9967..10812)
                     /gene="nhoA"
                     /locus_tag="ACHKAU_004007"
     CDS             complement(9967..10812)
                     /gene="nhoA"
                     /locus_tag="ACHKAU_004007"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO5532978.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10985..11554
                     /locus_tag="ACHKAU_004008"
     CDS             10985..11554
                     /locus_tag="ACHKAU_004008"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO5532979.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11558..11785)
                     /gene="pptA"
                     /locus_tag="ACHKAU_004009"
                     /pseudo
     CDS             complement(11558..11785)
                     /gene="pptA"
                     /locus_tag="ACHKAU_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
BASE COUNT         2863 a         3054 c         3090 g         2888 t
ORIGIN      
        1 aaataaccca cattgatact aataatacat ccgatattgg aactttatcc cccataataa
       61 aacagagcaa aaacttaaat tttttaaatg tttctgggaa gaacaattgc cctactatgc
      121 agctcatgtt atttaatttg ttttccccag cacttaagct taatactggc ctggcaattc
      181 tttcgcctgg tgcatttgaa gttcactctg acggaataga tgtggataac gaattgtttc
      241 actatcctat taaaaaagca tataccccat ataatataca cacttacaag acagaggaag
      301 ttgtaaacca gaggaatata aaagttaaaa acatgacctt agatgaaata aacaatactt
      361 actgtaataa cgattattac aatcaggcaa taagagagga accgatagac cttctggaca
      421 gatcgttttc ctccagttca tggccttttt agtgaataaa tcatcactaa ttctataagt
      481 gtctggtctt gttgataata tagttaatat gtaacccttt agattcacga tctgaagggt
      541 tgaaacatga catacgttca aagattaacc cccgtttgaa ggttagcttg ttagttacat
      601 ttagtaacac atatctggct acttaatccc cattctgccc ccgcggttac gctttcttat
      661 ctattctttc aatcagcact tcacccaatt catccttaat tctaataact ggctattgcg
      721 ccttacctcc tgttttgtct tttttcatta ccaatgtgtg catgtgagga acaatatggc
      781 actgcaaaat gagaaaaata gtcgttatct tttacgcgac tggaaaccag aaaatccggc
      841 cttctgggaa aataaaggaa aacatattgc tcgaagaaac ctctggatat cagtcagttg
      901 tctacttctt gccttctgtg tctggatgct atttagcgca gttaccgtta atctcaataa
      961 aatcggtttt aattttacta ccgatcaact ctttttatta accgcattac cctccgtttc
     1021 tggcgcatta ttgcgtgttc cctactcctt tatggtgcct atattcggtg gacgccgatg
     1081 gacggttttt agtactgcaa tcctgattat tccttgcgtc tggctcggaa ttgccgtgca
     1141 aaatccgaat actccttttg ggatatttat cgttatcgct ttgctatgcg gttttgcagg
     1201 tgcaaacttt gcttcgagca tgggcaatat cagtttcttc tttccaaaag ccaaacaagg
     1261 gagcgctctt gggattaatg gcggattagg aaacttaggt gtaagtgtaa tgcagctggt
     1321 tgcaccgctg gtcatttttg tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca
     1381 ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg atttgggtac cgctactggc
     1441 gattgccacg atcgccgcat ggtcagggat gaatgatatc gccagttcac gcgcctcaat
     1501 tgccgaccag ctccctgtct tacaacgcct gcatctctgg ctgctgagcc tgctttacct
     1561 tgccaccttc ggttcgttta tcggtttttc tgcgggtttt gccatgctgg caaaaaccca
     1621 gttcccggat gtgaatattc tgcgcctggc gttctttggc ccatttatcg gtgccatcgc
     1681 gcggtcggtt ggtggtgcta tttccgataa gttcggcggc gtgcgggtga cgttgatcaa
     1741 ctttattttt atggcgattt tcagtgccct gctgttcctt accttaccgg gcacaggctc
     1801 cggtaatttc atcgcctttt acgccgtatt tatggggctg tttctgactg cgggtctggg
     1861 aagtggttct actttccaga tgatcgccgt catctttcgc cagataacca tttatcgggt
     1921 aaagatgaaa ggcggtagtg atgagcaagc tcagaaagaa gccgtcaccg aaacggcggc
     1981 ggctctgggc tttatctcag ccattggcgc agtgggcggc ttttttattc cgcaggcgtt
     2041 tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt
     2101 ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt cggcggaagt tcagccaaaa
     2161 ataagagatc gatgcctgat gtacaaccgt gtcgcatccg acaaggcatc agatcccaat
     2221 tcattcctgg agcaggagtc atgtcatgag taaacttttg gatcgctttc gctacttcaa
     2281 acaaaagggc gaaacctttg ccgatggtca cggacaggtg atgcatagca accgcgactg
     2341 ggaggacagc tatcgccagc gttggcagtt cgacaaaatc gtgcgttcca cccacggtgt
     2401 taactgtaca ggctcctgta gctggaaaat ctacgttaaa aatggtctgg tgacctggga
     2461 aatccaacag accgactacc cgcgcactcg ccctgacctg cccaatcatg aacctcgcgg
     2521 ctgcccgcgt ggcgcaagtt actcctggta tctttacagc gctaaccgcc tgaaataccc
     2581 gctcattcgt aaacgactga ttgaactgtg gcgcgaagcc ctcaagcaac acagcgatcc
     2641 ggtactggcg tgggcatcga ttatgaacga tccgcaaaag tgcctgagct acaaacaagt
     2701 gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa ctaaaccagc tgattgccgc
     2761 cgctaacgtc tggaccatca aaacctacgg cccggatcgc gttgccggtt tctcgccgat
     2821 cccggcgatg tcgatggttt cttacgccgc cggaacgcgt tatctgtcgc tgcttggcgg
     2881 cacctgttta agtttctacg actggtattg cgacctgccg cccgcctcgc cgatgacctg
     2941 gggcgagcaa accgacgtac cggaatctgc cgactggtat aactccagct acatcatcgc
     3001 ctgggggtct aacgtaccgc agacacgtac gccggacgcc cacttcttta ccgaagtacg
     3061 ctacaaaggc actaaaacca tcgccattac ccctgactac tctgaagtgg ccaaattgtg
     3121 cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc ctggcgatgg caatgggcca
     3181 tgtgatttta aaagagtttc atctcgataa tcccagcgac tactttatca actactgccg
     3241 ccgctacagc gacatgccga tgctggtaat gctggagcct cgcgacgatg gtagctacgt
     3301 tcccgggcgc atgatccgcg catctgacct ggtggatgga ctgggcgaaa gcaacaatcc
     3361 gcagtggaaa accgtagcag ttaataccgc aggtgaattg gtagtgccga acggttcgat
     3421 tggtttccgc tggggagaaa aaggcaaatg gaatctggaa tccattgccg ccggtacgga
     3481 aaccgaattg tcgttaaccc tgctcggtca acatgacgct gttgcaggcg tggccttccc
     3541 ctactttggc ggcattgaaa atccgcattt tcgcagcgta aaacacaatc cggtgctggt
     3601 gcgccaattg cccgttaaaa acctgacgtt agtcgatggc aacacctgtc cggtggtcag
     3661 cgtttatgat ttggtactgg cgaattacgg cctcgatcgc gggctggaag atgaaaacag
     3721 tgcgaaagat tacgctgaaa tcaaaccgta caccccagcc tggggtgagc aaattaccgg
     3781 cgtgccgcgc cagtatattg aaaccatcgc ccgtgaattt gccgataccg cccataaaac
     3841 gcatgggcgc tcgatgatta tcctcggcgc aggtgttaac cactggtatc acatggacat
     3901 gaactaccgc gggatgatca atatgctgat cttctgcggt tgtgtcgggc aaagcggtgg
     3961 cggctgggca cactatgtcg gccaggaaaa actgcgccca caaaccggct ggttgccgct
     4021 ggcctttgcg ctcgactgga accgaccacc acgccagatg aacagtacct cgtttttcta
     4081 caatcattcc agccaatggc gctatgaaaa agtctctgct caggagttac tttcaccgct
     4141 cgccgatgcc agtaagtaca gcggtcatct gattgatttc aacgttcgcg ccgaacgtat
     4201 gggctggcta ccttctgcgc cgcagctggg gcgtaacccg ctcgggatta aagctgaagc
     4261 cgacaaggcc ggattatccc ccacagaatt taccgcccag gcgctgaaat cgggcgattt
     4321 acgtatggcc tgcgaacaac cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg
     4381 gcgctctaac ctgcttggct cctccggcaa aggccacgag tatatgcaga agtatctgct
     4441 ggggaccgaa agcgggattc agggcgagga actcggtgcc agcgacggga tcaaaccgga
     4501 agaagtcgag tggcaaactg cggcgattga aggcaagctc gacctgctgg tgacgctcga
     4561 cttccgcatg tccagtacct gcctgttctc cgatatcgtt ctgcccaccg ccacctggta
     4621 cgaaaaagac gatatgaaca cctcggatat gcatccgttt attcatccgc tttctgcggc
     4681 ggtcgatccg gcgtgggaat cacgcagcga ctgggaaatc tacaaaggta tcgccaaagc
     4741 attttcgcaa gtgtgcgtgg gtcatcttgg caaagaaacc gacgtggtat tacaacccct
     4801 actgcatgac tctccggcag agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg
     4861 cgaatgcgat ctgattccag gcaaaaccgc tccgaatatt gtggcggtgg agcgcgacta
     4921 ccctgctacg tatgaacgct ttacctcgct cgggccattg atggacaaac ttggcaatgg
     4981 cggtaaaggg atttcgtgga atacgcagga tgaaatcgat ttcctcggca aactcaatta
     5041 caccaagcgt gatggcccgg cacaggggcg tccgctgatt gacaccgcca ttgacgcttc
     5101 agaagtgatt ctggcactgg caccggaaac caacggtcat gttgcagtca aagcgtggca
     5161 ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg gcgctgcaca aagaggacga
     5221 gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa attatctcca gccccacctg
     5281 gtccggtctg gaaagcgatc acgtatccta taacgcggga tacaccaacg ttcatgagtt
     5341 aattccgtgg cgcacgctgt cgggacgcca gcagctctat caggatcatc cgtggatgcg
     5401 tgcttttggt gaaagcctgg tggcttatcg cccgcctatc gacacccgta gcgtcagtga
     5461 gatgcgccag ataccgccaa acggcttccc ggaaaaagca cttaacttcc tgacgccgca
     5521 ccagaaatgg ggcattcact caacctacag tgaaaacctg ctaatgctga cgctctctcg
     5581 cggtggaccg attgtctgga tcagcgaaac agatgcccga gaactgacca ttgtcgataa
     5641 cgactgggtg gaagtattca acgccaacgg cgcgctgact gcccgcgcgg tggtcagcca
     5701 acgtgtaccg ccgggcatga ccatgatgta tcacgcccag gaacgcatta tgaatattcc
     5761 tggttcggaa gtaactggca tgcgcggcgg cattcataac tcggtcaccc gcgtttgccc
     5821 gaaaccaacg catatgattg gcggttacgc gcagctggcc tggggcttta actactacgg
     5881 caccgtcgga tcgaaccgcg atgagttcat catgatccgg aagatgaaga acgttaactg
     5941 gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa aaatgaaaat ccgttcacaa
     6001 gtcggcatgg tgcttaacct cgacaaatgt atcggctgcc atacctgttc ggtgacctgt
     6061 aaaaacgtct ggaccgggcg cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc
     6121 aaaccgggca ttggttatcc gaaaaactgg gaagatcagg aagagtggca aggcggctgg
     6181 gtccgcgatg tgaatggcaa gatccgtccg cgtctgggca gcaagatggg cgtaataacc
     6241 aaaatcttcg ccaacccggt ggtgccgcag attgatgatt actacgaacc tttcaccttc
     6301 gactacgaac atttgcatag cgcaccggaa ggcaaacata tccctactgc tcgcccgcgt
     6361 tcactgattg acggcaaacg gatggacaaa gtgatctggg gcccaaactg ggaagaactg
     6421 ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact tcgaggccat gcaaaaggag
     6481 atgtacgggc agtttgaaaa caccttcatg atgtacctgc cgcgcctgtg cgaacactgc
     6541 ctcaatccca gttgcgtggc gacctgccca agcggcgcta tctacaaacg cgaagaagac
     6601 ggcattgtgc tgattgatca ggataaatgc cgtggctggc gtttgtgcat tagcggttgt
     6661 ccgtacaaaa aaatctactt caactggaaa agcggcaagt cagaaaaatg tatcttctgt
     6721 tatccgcgaa ttgagtcagg ccaacccacc gtgtgctcag aaacctgcgt gggtcgcatc
     6781 cggtatctgg gcgtgctgct ttacgacgcc gaccgcattg aggaagcggc gagcaccgag
     6841 cgcgaagttg acctctatga acgtcagtgc gaagtattcc tcgatccaca cgatccctca
     6901 gtgatcgagg aagccctgaa acaaggtatt ccacaaaacg tgattgaagc tgcccagcgt
     6961 tcgcctgtct acaaaatggc gatggactgg aaactggcgc taccgctgca ccctgaatat
     7021 cgcaccctgc caatggtctg gtacgttcct ccgctgtcgc cgattcagtc ctacgcagat
     7081 gcgggcggtt tgccgaaaag cgaaggcgtg ctgcccgcca tcgaaagcct gcgtattccg
     7141 gtgcaatatc tcgccaatat gttgagtgcc ggcgataccg gtccggtact gcgggcgctg
     7201 aaacggatga tggcgatgcg ccactatatg cgttcacaaa ccgtggaagg cgttactgat
     7261 actcgtgcca tcgacgaagt aggcctgagc gtcgcccagg tcgaagagat gtatcgttac
     7321 ctcgccattg ccaactatga agatcgtttt gtcatcccga cgagccatcg ggaaatggcg
     7381 ggcgatgcct tcgcagaacg caacggctgc ggttttacct ttggcgacgg ttgccacggt
     7441 tcggacagta aattcaacct gttcaacagt agccgtatcg atgccatcaa catcaccgaa
     7501 gtgcgcgaca aagcggaggg cgaataatgc agatcctcaa agtgatcggc ctgttgatgg
     7561 agtatccgga cgagctgttg tgggaatgca aggaggacgc gctggcgttg atccgcagcg
     7621 acgcgccgat gcttacggat ttcacccaca acctgcttaa cgcgccgctg ctggataaac
     7681 aggccgaatg gtgcgaagtg tttgaccgtg gacgtaccac atcgctgctg ctgttcgaac
     7741 atgttcatgc cgagtcccgc gatcgcggcc aggcaatggt ggacctgctg gcggagtatg
     7801 aaaaggtcgg cctgcagctg gattgtcggg aactgcccga ttatctaccg ctgtatctgg
     7861 agtatttaag cgtactgcct gacgatcagg cgaaagaagg attgctcaat gttgcgccga
     7921 tcctcgccct gcttggcggt cgcttaaaac aacgcgaggc accgtggtac gcgttgtttg
     7981 atgctctgct gcaactggcg ggaagctccc tttcaagtga cagtgtcaca aaacaagtaa
     8041 acagcgaaga gcgcgatgac acccgccagg cgcttgatgc agtgtgggaa gaggaacagg
     8101 tgaagtttat tgaagataac gccacggcgt gtgacagctc gccgcttaat caatatcagc
     8161 gacgctttag ccaggatgtc gcgccgcaat atgtcgacat cagtgcggga ggtgggaaat
     8221 gattcagtat ctgaacgtct ttttttacga tatctacccg tacatttgtg cgacggtgtt
     8281 tttcctcggc agctggctgc gctacgacta cgggcagtac acctggcgcg cctcctcaag
     8341 tcaaatgctc gataaacgcg agatggtgat atggtcgaat ctgttccata tcggcatttt
     8401 ggggattttc ttcgggcact tgttcggcat gttaacgccg cactggatgt acgcgtggtt
     8461 tttgccagtg gcagcgaaac aactaatggc gatggtgctc ggcggtattt gcggtgtgtt
     8521 gacattgatt ggcggcgcag gactgctgtg gcgcaggctg acaaatcagc gcgtgcgtgc
     8581 aacttccact acgccggata ttatcatcat gagcattttg ctgatccagt gtctgctggg
     8641 gctaagtacc ataccgtttt cagcacagta tcctgatggt agcgaaatga tgaagctggt
     8701 gggttgggcg caaagcattg tcactttccg tggtggatcg tcagaaatgc ttaacggcgt
     8761 agcgttcgtt ttccgcctgc atctggtatt gggaatgacc atcttcctgc tcttcccgtt
     8821 caccagattg gtgcatgtgt ggagcgcgcc gtttgagtac tttactcgtc ggtatcagat
     8881 tgtgcgttcg cggcgataat ttcaccctgg ccggatagcc cttcgctctc cggccacttg
     8941 caggctatga tagttctcca gtcacaggag aacgttaatg aaaccgcagg tgtaccacgt
     9001 cgatgctttt acctctcaac catttcgcgg caattctgcc ggtgttgttt tccccgccga
     9061 taatcttagc gaagcgcaaa tgcagcttat cgcccgcgag ttaggccatt cggaaactgc
     9121 ttttctgctg cacagcgacg acagcgatgt gcgcatccgc tactttacgc caacggttga
     9181 agtgccgatt tgcggtcacg cgacagtagc tgcgcactat gtacgtgcga aggtgttagg
     9241 tttaggaaat tgcacgatct ggcaaacatc gctggcagga aaacatcgcg tgactatcga
     9301 aaagcacaac gatgattatc gtatttcgct ggaacaaggt acgccgggct ttgagccacc
     9361 gctggaaggt gaaacacgtg aggcgattat caacgcgctt catctcactg aagacgatat
     9421 tttgccgggc ttgccaattc aggtcgcaac aacaggacac tcaaaagtga tgatcccgtt
     9481 gaaaccggaa gtggatatcg acgccctttc gcctgatctt gctgcgctga gcgctatcag
     9541 caaacagatt ggctgcaatg gtttcttccc gttccagatc cgcccaggca aaaacgaaac
     9601 cgatggtcgt atgttctcgc ctgcgattgg tattgtggaa gatccggtga ctggcaatgc
     9661 caatggcccg atgggtgcat ggttggtaca tcacaacgta ttgccccacg atggcaacgt
     9721 gttatgtgtt aaaggccatc aggggcgcgc attggggcgt gacggcatga ttgaagtgac
     9781 ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt tcaggcacag cggtgatttt
     9841 gtttcatgcc gaatgggcaa ttgaactctg aaaccgttgc ctgatgcgct tcgcctgtca
     9901 ggtctacaac cgagctaatg tatgccggat aaggcgttta cgccgcatcc ggcaacctga
     9961 cataaattat tttcccgcct ccgggtgcgt atcaaacgcc gccatcacca gcgctaactc
    10021 atccacggta aagccatgtt tcgcatcatc cacgcccaga ccaaactgtt cttgcatcac
    10081 agcatataat gacgccacat ccggtagatt tcgctgctcc accgcgtgcc cattttcata
    10141 atgggtaaaa tgaaaattgg tcagcgttag cttgccaccg tccggcaaat ggcggcacat
    10201 cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac
    10261 ataatcgctt tgttgctgct cgcagagatc aaaacggtac atcgactgcc agtgctgatg
    10321 atgattaaac tgcaacaccc aatcatcacc ctcctgcaac aaccgataat ctccgtgtgg
    10381 cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa
    10441 accgacatca gcaatccatt tttcctcttc cagttccacc aacagcaaac gatgggtgcg
    10501 cggcggtaat gctggcggat ttgataacac tacgcgccct aacaagctgc gaacgttaaa
    10561 ctccagctcg cgtaacaccc gctcaaacac gccattctgc tcaaaacagt aaccgccacg
    10621 acgggctatc accagtttct cttccagcga ttgatcatca agctgtattt ccctcggcag
    10681 caaaacgtcg aggttttcaa acggaatagt gcaattgtgt ttcaggtgca atgcacgaag
    10741 cgtatcaata ttgaccgcag cagctcccga ccagttaata cgggcaaaat agtgattcag
    10801 aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa cttataacct ggaatagacg
    10861 ccgcaccggc tattttgtgc gatcgcggac atgcatcacg tttgcaaatc gccaatcccg
    10921 ttaatttcgt tacttattgc gttacggact acgcttaaaa aaaccttctt cgcgaggcga
    10981 taccatgagc cgattcatcc ccatagaatt acaccatgcc agccgtctgt taaatcatgg
    11041 cccaactgtc atgatcacca gtttcgatga gcaatcccag cggcgtaaca ttatggcagc
    11101 agcctggtca atgccggtgg agtttgaacc gccacgcgtg gcgattgtgg tagataaatc
    11161 gacatggacc agggagttga ttgaacgtaa cggtaaattt ggcatcgtta tcccgggcgt
    11221 tgcagcaact aactggacgt gggcagtggg aagcgtgtcg ggtcgtgaag aagataaatt
    11281 taattgctat ggcattccgg tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga
    11341 gaaatgcctg gcgtggatgg agtgccgcct gctacctgcg acttctgcgc aacaaaaata
    11401 cgacacgctg tttggtgagg tggtatcagc agcggcagac gcacgggtat ttgtcgaagg
    11461 ccgctggcag tttgatgatg ataagctcaa tacgttgcat catttaggtg ctggcacgtt
    11521 tgttaccagc ggcaagcgag ttacggcggg ttaagcatta catgctgtaa ccgggtttct
    11581 ttatcaaagc ctccatttgg ggcgcgattt cggcatccca gatagcttgc caagattctg
    11641 gttgaatctg ctgtagagca atgcttatcg agctatcttt actgttcaga tggcgaataa
    11701 taacgtcggt aatatctgca gcaagtgctg ctttttattg ttcgtccagt tcacgcggaa
    11761 aacatttaat gtcgatgtgc ggcatgattg tgctcctttt gaaagatgtc agtaaaatta
    11821 acacacattg tgaatgtcgt gtgtccggta atgcgttttt acgacaataa tgccagggca
    11881 agattacgaa agcct
//
LOCUS       DAZFJF010000129        11504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFJF010000129 DAZFJF010000000
VERSION     DAZFJF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.129"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            112..1254
                     /gene="yghO"
                     /locus_tag="ACHKAU_004010"
     CDS             112..1254
                     /gene="yghO"
                     /locus_tag="ACHKAU_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO5532980.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1261..2025)
                     /gene="glcC"
                     /locus_tag="ACHKAU_004011"
     CDS             complement(1261..2025)
                     /gene="glcC"
                     /locus_tag="ACHKAU_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO5532981.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2276..3775
                     /gene="glcD"
                     /locus_tag="ACHKAU_004012"
     CDS             2276..3775
                     /gene="glcD"
                     /locus_tag="ACHKAU_004012"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO5532982.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKLPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3775..4827
                     /gene="glcE"
                     /locus_tag="ACHKAU_004013"
     CDS             3775..4827
                     /gene="glcE"
                     /locus_tag="ACHKAU_004013"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO5532983.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4838..6061
                     /gene="glcF"
                     /locus_tag="ACHKAU_004014"
     CDS             4838..6061
                     /gene="glcF"
                     /locus_tag="ACHKAU_004014"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO5532984.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6066..6470
                     /gene="glcG"
                     /locus_tag="ACHKAU_004015"
     CDS             6066..6470
                     /gene="glcG"
                     /locus_tag="ACHKAU_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO5532985.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6492..8663
                     /gene="glcB"
                     /locus_tag="ACHKAU_004016"
     CDS             6492..8663
                     /gene="glcB"
                     /locus_tag="ACHKAU_004016"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO5532986.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8891..9136
                     /gene="traT"
                     /locus_tag="ACHKAU_004017"
                     /pseudo
     CDS             8891..9136
                     /gene="traT"
                     /locus_tag="ACHKAU_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(9169..10911)
                     /gene="cptA"
                     /locus_tag="ACHKAU_004018"
     CDS             complement(9169..10911)
                     /gene="cptA"
                     /locus_tag="ACHKAU_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5532987.1"
                     /translation="MQKTLTKHEHVFSWKALGWALIYFWYFSTLLQIIICLTGYSGTN
                     GLRDSLLFSSLWLIPVFLFPERIKVTAALIGVVLWGTSLAALCYYLIYGQEFSQSVLF
                     VMFESNTSEASEYFSQYFSLKILFISTIYTAIAILLWTRLSPIYIPSYWRYIIPLFII
                     YGLILHPVLINTVVKNRTIDNTLGRLASRMEPAAPWQFITGYYQYRLQLNSLNRLLEE
                     NNALPALKNFNDISGDTPRTLVLVIGESTQRGRMSLYGYPRKTTPGLDELYKTEQNMF
                     VFNNVVSSRPYTIEVLQQVLTFADEQQPELYLTQPSLMNIMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLAPFSEVLADPAPKKFVIIHLLGT
                     HIKYKYRYPEGQGVFDGDREHAPEGLTDNELELYNDYDNANIFNDHVVATLIKTFKAT
                     APDGFLFYFSDHGEEVFDTPPHKMLGRNEDTPTRPMYNIPFILWASEKWLLNRHHTFS
                     EYINRKYSLADFIHTWSDLAGLRYDNYEPERSILNQHFKEITRWIGNPYKKNALTDYD
                     TLPYAELRVNQASN"
     gene            11368..>11504
                     /locus_tag="ACHKAU_004019"
     CDS             11368..>11504
                     /locus_tag="ACHKAU_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5532988.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ET"
BASE COUNT         2883 a         2771 c         3102 g         2748 t
ORIGIN      
        1 taataaatac tatttattct ttgtttgtat tcattgggtt gattattaag gtgtcgttcg
       61 ttgctgttgc aacctgtttt tcaccgttaa ttatttgtta tccacagaga tatggaatgc
      121 gatctgctga tgattaaaat tgaaaaagta attaataaaa atgaccttaa ggcatttatc
      181 gcttttccgt catcacttta tcccgacgat ccaaactgga tacccccttt attcatagaa
      241 cgcaacgagc atttgtctgc gaaaaatccg gggacggacc atatcatctg gcaggcgtgg
      301 gtggcaaaaa aagcggggca gatagttggg cgtattaccg cgcaaataga taccttgcac
      361 cgtgagcgtt acggtgaaga taccggtcat ttcggcatga ttgacgccat tgatgatccg
      421 caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt tgaagtcaca aggtgcaagt
      481 aagatcagcg gtcctttcag cctgaatatc aatcaggaaa gcggattact gattgaaggt
      541 tttgacacac caccctgtgc gatgatgcca cacggcaaac cgtggtatgc cgcgcatatt
      601 gaacaactgg gttatcacaa aggcattgat ttactggcgt ggtggatgca gcgaaccgat
      661 ctcactttct ctccggcgct aaaaaaactg atggatcagg tgcgcaaaaa ggtgaccatt
      721 cgctgcatca atcgtcagcg gtttgccgaa gaaatgcaga tcctgcgtga gattttcaac
      781 tctggctggc agcacaactg gggatttgtg ccgtttaccg aacatgaatt cgcgaccatg
      841 ggcgatcaac ttaaatatct ggtgccggac gatatgatct atatcgctga gattgattct
      901 gcaccctgcg cgtttattgt cggcttgccg aacatcaacg aggcgattgc cgatctgaac
      961 ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg
     1021 cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt atcagttcag ccgcatcggc
     1081 ccggtgattg cgctgttatt gattgaagcc ttacgcgatc cgtttgcccg ccggaagatc
     1141 gatgcgctgg agatgtcatg gatcctcgaa accaatacag gcatgaataa catgctggaa
     1201 cgcattgggg cggagccgta caagcgttac cgattgtatg aaaagcagat ttaataccag
     1261 ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc aaatggtggc cttccagctc
     1321 gatatcgtgg agattctttt tcacggtccg cacatgatcg cgtgctgcgc gctgggcgac
     1381 gtgcggcagc cgctgcaaca ccgcgttgta gatcctcgca tgctggcgat cgatctgctt
     1441 tttttgctgt ggtcgatggt agagattatt tacgctggca aacactgaat taaacatcag
     1501 atcggtcaat gattgcagcg taaacaccag cacctgattg tgagaagcct gacaaatggc
     1561 gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc gaaatctctt tgttgttctc
     1621 actggcggcg agcatttttt cataacagcg ggttatcaca acaaaatcag cctgcgttcc
     1681 cagcgttgcc gccagccttg ccgattcgcc ctccagtaat gcgcgaacgt cgagcagatc
     1741 gtacagcgtt cgcggctgcg tactgaacag atggatcagc ggactggtgt cctgcacccg
     1801 attaagccgt gcgacacgag aatcgcgacc ctgcgccgtt tcaataatcc cgcgcccgcg
     1861 cagcacggtc agcccttcac gcagtgcgga gcgtgagaag ccgagctttt cacacagtcg
     1921 acgttccgag ggaagtggct gaccgacctt cagtacgccg tcgataatta accgttcgat
     1981 actctctgca accacttcgc aaatagggcg acgttcatct ttcattcccg gacctcgtgc
     2041 acaggtagga ccaatttttc tgttcttaac tcgcaaaaca cgcacatcac gtaagtgatt
     2101 atgttacatc aatttaacat tgagttaacc aaggcaaggt cacagagctg gaaaaaaaat
     2161 ggtctgaccg gtagctaaag agatagacga aaacgaaaag cccgcttaat aactgttcac
     2221 agaagcagcg cgcaaaaatc agctgccaca caacacaaca aagcgaagcc tactcatgag
     2281 catcttgtac gaagagcgtc ttgatggcgc tttacccgat gtcgaccgca catcggtact
     2341 gatggcactg cgtgagcatg tccctggact tgagatcctg cataccgatg aggagatcat
     2401 tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt ccattactgg ttgttctgcc
     2461 taagcaaatg gaacaggtga cagcgattct tgctgtctgc catcgcctgc gtgtaccggt
     2521 ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg ctgccgctgg aaaaaggtgt
     2581 gttgttggtg atggcgcgct ttaaagagat cctcgacatt aaccccgttg gtcgccgcgc
     2641 gcgcgtgcag ccaggcgtgc gtaacctggc gatctcccag gccgttgcac cgcataatct
     2701 ctactacgca ccggaccctt cctcacaaat cgcctgttcc attggcggca acgtggcaga
     2761 aaatgccggc ggcgtccact gcctgaaata tggtctgacc gtacataacc tgctgaaaat
     2821 tgaagtgcaa acgctggacg gcgaggcgct gacgctggga tcggacgcgc tggattcacc
     2881 tggttttgac ctgctggcgc tgttcaccgg atcggaaggt atgctcggcg tgaccaccga
     2941 agtgacggta aaactgctgc cgaagctgcc cgtggcgcgg gttctgttag ccagctttga
     3001 ctcggtagaa aaagccggac ttgcagttgg tgacatcatc gccaatggca ttatccccgg
     3061 cgggctggag atgatggata acctgtcgat ccgcgcggcg gaagatttta ttcatgccgg
     3121 ttatcccgtc gacgccgaag cgattttgtt atgcgagctg gacggcgtgg agtctgacgt
     3181 acaggaagac tgcgagcggg ttaacgacat cttgttgaaa gcgggcgcga ctgacgtccg
     3241 tctggcacag gacgaagcag agcgcgtacg tttctgggcc ggtcgcaaaa atgcgttccc
     3301 ggcggtagga cgtatctccc cggattacta ctgcatggat ggcaccatcc cgcgtcgcgc
     3361 cctgcctggc gtactggaag gcattgcccg tttatcgcag caatatgatt tacgtgttgc
     3421 caacgtcttt catgccggag atggcaacat gcacccgtta atccttttcg atgccaacga
     3481 acccggtgaa tttgcccgcg cggaagagct gggtgggaag atcctcgaac tctgcgttga
     3541 agttggcggc agcatcagtg gggaacatgg catcgggcga gaaaaaatca atcaaatgtg
     3601 cgcccagttc aacagcgatg aaatcacgac cttccatgcg gtcaaggcgg cgtttgaccc
     3661 cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta caccgctgtg ctgaatttgg
     3721 tgccatgcat gtgcatcacg gtcatttacc tttccctgaa ctggagcgtt tctgatgcta
     3781 cgcgagtgtg attacagcca ggcgctgctg gagcaggtga atcaggcaat tagcgataaa
     3841 acgccgctgg tgattcaggg cagcaatagc aaagcctttt taggtcgccc tgtcaccggg
     3901 caaacgctgg atgtgcgttg tcatcgcggc attgttaatt acgacccgac cgagctggtg
     3961 ataaccgcgc gtgccggaac gccgctggtg gcgattgaag cggcgctgga gagcgccggg
     4021 caaatgctcc cctgcgagcc gccgcattat ggtgaagaag ccacctgggg cgggatggtc
     4081 gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt cggtccgcga ttttgtcctc
     4141 ggcacgcgca tcattaccgg cgctggaaaa catctgcgtt ttggtggcga agtgatgaaa
     4201 aacgttgccg gatacgatct ctcacggtta atggccggaa gctacggttg tcttggcgtg
     4261 ctcactgaaa tctcgatgaa agtgttaccg cgaccgcgcg cctccctgag cctgcgtcgg
     4321 gaaatcagcc tgcaagaagc cattaacgaa atcgcccagt ggcaactcca gccgctaccc
     4381 attagtggct tatgttactt cgacaatgcg ttgtggatcc gccttgaggg cggcgaagga
     4441 tcggtaaaag cggcgcgtga actgctgggg ggcgaagagg ttgcgggtca gttctggcag
     4501 caattgcgtg aacaacaact gccgttcttc tcgttaccag gtaccttatg gcgcatttca
     4561 ttacccagcg atgcgccgat gatggattta cccggcgagc aactgatcga ctggggcgga
     4621 gcgttacgct ggctgaaatc gacagccgag gacaatcaaa tccatcgcat cgcccgcaac
     4681 gctggcggtc atgcgacccg ctttagtgcc ggagatggtg gctttgcccc gctatcggct
     4741 cctttattcc gctatcacca gcagcttaaa cagcagctcg acccttgcgg cgtgtttaac
     4801 cccggtcgca tgtacgcgga actttgagga gcaggctatg caaacccaat taactgaaga
     4861 gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc ctgcgcgcct gtgttcactg
     4921 cggattttgt accgcaacct gcccaaccta tcagcttctg ggcgatgaac tggacgggcc
     4981 gcgcgggcgc atctatctga ttaaacaggt gctggaaggc aacgaagtca cgcttaaaac
     5041 acaggagcat ctcgatcgct gcctcacttg ccgcaattgt gaaaccacct gtccttctgg
     5101 tgtgcgctat cacaatttgc tggatatcgg gcgtgatatt gtcgagcaga aagtgaaacg
     5161 cccactgccg gagcgaatgc tgcgcgaagg attgcgccag gtagtgccgc gtccggcggt
     5221 cttccgtgcg ctgacgcagg tagggctggt gctgcgaccg tttttaccgg aacaggtcag
     5281 agcaaaactg cctgctgaaa cggtgaaagc taaaccgcgt ccgccgctgc gccataagcg
     5341 tcgggtttta atgctggaag gctgcgccca gcctacgctt tcgcccaaca ccaacgcggc
     5401 aactgcgcga gtgctggatc gtctggggat cagcgtcatg tcagctaacg aagcaggctg
     5461 ttgtggcgcg gtggactatc atcttaatgc gcaggagaaa gggctggcac gggcgcgcaa
     5521 taatattgat gcctggtggc ccgcgattga agcaggtgcc gaggcaattt tgcaaaccgc
     5581 cagcggttgc ggcgcgtttg tcaaagagta cgggcagatg ctgaaaaacg atgcgttata
     5641 tgccgataaa gcgcgtcaag tcagtgaact ggcggtcgat ttagtcgagc ttctgcgcga
     5701 ggaaccgctg gaaaaactgg cagttcgcgg cgataaaaaa ctggccttcc actgcccgtg
     5761 taccctacaa catgcgcaaa agctgaacgg cgaagtggaa aaagtgttgc ttcgtcttgg
     5821 atttacctta acggacgttc ccgacagcca tctgtgctgc ggttcagcgg gaacatatgc
     5881 gttaacgcat cccgatctgg cacgccagct gcgggataac aaaatgaatg cgctggaaag
     5941 cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc cagacgcatc tggcgagcgc
     6001 cggtcgtacc tcagtgcgtc actggattga aattgtagaa caagcccttg aaaaggaata
     6061 aaaaaatgaa aactaaagtc attcttagcc agcaaatggc gagtgcaatt attgccgcag
     6121 gtcaggaaga ggcgcagaaa aataactggt cggtttccat tgctgttgcc gatgacggcg
     6181 gtcatctgct ggcgttaagt cgcatggacg attgcgcgcc gattgcggct tacatctccc
     6241 aggagaaagc gcgtaccgcc gcgctggggc gtcgtgaaac taagggctat gaagagatgg
     6301 tgaacaacgg acgtaccgcg ttcgtgactg cgccgttact gacgtcgctg gaaggcggcg
     6361 taccggttgt tgtggatggg caaattattg gtgccgtggg cgtttctggt ttaaccggag
     6421 cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt ggcgaaataa gcgaaaacga
     6481 ggagagaaac aatgagtcaa accataaccc agggccgttt acgcattgac gccaatttta
     6541 aacgttttgt ggatgaagaa gttttaccgg gaacagggct ggacgctgcg gcgttctggc
     6601 gcaattttga tgagatcgtt cacgatctgg caccagaaaa tcgtcagttg ctggcagaac
     6661 gcgatcgcat tcaggcagcg cttgatgagt ggcatcgcag caatccgggg ccggtaaaag
     6721 ataaagcggc ctataaatct ttcctgcgtg aactgggcta cctggtgccg caaccggagt
     6781 gcgtgacggt ggaaaccacg ggcattgaca gcgaaatcac cagtcaggca gggccacagc
     6841 tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc ggcgaacgct cgctggggct
     6901 cactgtacga tgcgttatac ggcagcgaca tcatcccgca ggaaggggcg atggtcagcg
     6961 gctacgatcc gcagcgcggt gagcaggtta tcgcctgggt tcggcgtttc ctcgatgaat
     7021 ctctaccgct ggaaaacggc agctatcagg atgtggtggc gtttcaggtg gtcgataaac
     7081 aattacgcat ccagttgaaa aatggtaaag aaaccacgtt acgtaccccg gcgcagtttg
     7141 tcggttaccg tggcgatgcc gctgcgccga cctgcatttt gctgaaaaat aacggcctgc
     7201 atattgaact gcaaattgat gccaacggac ggattgggaa agacgatgcg gcgcacatca
     7261 acgatgttat cgtcgaagct gcaatcagta ccattctcga ctgcgaagat tcggtcgcgg
     7321 cggttgatgc ggaagataaa atcctgctgt accgcaatct gctgggcctg atgcagggga
     7381 ctctccaaga gaaaatggag aaaaacggtc gacaaatcgt gcgtaaactg aatgacgatc
     7441 gtcagtacac cgccgccgat ggctctgaaa tttctctgca cggacgctcg ctgctgttta
     7501 tccgcaacgt gggtcatttg atgaccattc ctgtgatttg ggacagcgaa ggcaatgaaa
     7561 tcccggaagg cattcttgat ggcgtcatga ctggcgcgat tgccctctat gatttaaaag
     7621 tgcagaaaaa ctcgcgcact ggcagcgtct atattgtgaa accgaaaatg cacggtccgc
     7681 aggaagtggc gttcgccaac aaactgttta cccgcattga gacaatgctc ggtatggcac
     7741 cgaataccct gaaaatgggc attatggatg aagaacgtcg gacctcgctg aacttgcgta
     7801 gctgtatcgc tcaggcacgc aaccgcgtgg cgttcatcaa taccggtttc ctcgaccgta
     7861 ccggcgatga aatgcattcg gtgatggaag ctggcccgat gctgcgtaaa aatcagatga
     7921 aatcgacgcc gtggatcaaa gcctacgagc gtaataacgt gctttccggc ctgttctgtg
     7981 ggctgcgcgg taaagcgcaa atcggtaaag gcatgtgggc aatgccggac ctgatggcag
     8041 acatgtacag ccagaagggc gaccaactgc gtgccggggc aaacacagcc tgggttccgt
     8101 cgccaactgc cgctacgctc catgcgatgc actaccacca aaccaacgta cagagcgtgc
     8161 aagccaacat tgcccagact gagttcaatg ctgaatttga accgctgctg gacgatctgc
     8221 tgactattcc ggttgctgaa aacgctaact ggtcggcgca agagatccaa caagagctgg
     8281 ataacaacgt gcaggggatt ctggggtacg tggtgcgctg ggtggagcag gggattggtt
     8341 gttcaaaagt gccggatatt cacaatgtgg cgctgatgga agaccgcgca acgctgcgta
     8401 tctccagcca gcatatcgcc aactggttac gtcacggtat tctgaccaaa gaacaggtgc
     8461 aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca aaacgctgac gatccggctt
     8521 atcgtccgat ggcggggaat ttcgctaact cgtgtgcttt taaagctgcc agcgatttaa
     8581 tcttcctcgg cgtgaaacag ccaaacggct ataccgaacc gttattacac gcctggcgtt
     8641 tacgcgaaaa agaaagtcat taatagcgtc aaaaaaagcc ccggcaagtt ttattgtcgg
     8701 ggctatgata gtaactgcgg tagtacattt atcccggaaa taaccggagc gattatcagg
     8761 gtttaacaat attaaatacc cgtagcgcat tactagcggg cctataacaa caatatggac
     8821 atcttaacga tactctgcgc ctgcgtgtgc agaacagaaa cccttacccg atatacttgt
     8881 tatgccagag gtagcaattg cggcaaccga tctgactggc atggatgcag atgcaatggt
     8941 ggaagatata aattacacga taattacaga tgttcagatt accgaacgaa ctaaaaccta
     9001 tgtcagagca gataatgcta ccacttttcg gcagggaaca tcaggcaccg aaattcaggc
     9061 tagcacagaa accagcaacc aggttaatct gaaatttgaa ggggctaaac ctcttctgga
     9121 aatacaactg aaataaatat cctccggcta tgccggagga tatttatttt aatttgaagc
     9181 ctgatttaca cgcagctctg cgtagggcag ggtatcatag tcagttaacg catttttctt
     9241 ataggggttt cctatccagc gagtgatttc tttgaaatgc tggtttaata tggaacgctc
     9301 tggctcatag ttatcataac gtaaaccagc caaatccgac caggtatgga taaaatcagc
     9361 cagactgtat ttacggttaa tgtactcaga aaatgtatga tgtcgattaa gtagccattt
     9421 ttcagaggcc cagagaataa atggaatatt gtacataggt cgggttgggg tgtcttcgtt
     9481 acgtcccagc attttatgtg gtggcgtatc gaatacttcc tctccatggt cagaaaaata
     9541 gaaaagaaaa ccatcagggg ctgttgcttt gaaagttttg atcaacgttg caacaacatg
     9601 gtcattaaat atattagcat tatcgtaatc gttatataat tccagttcat tatcagtaag
     9661 tccttctggt gcatgttccc tgtccccatc gaatactccc tgaccttcag gataacggta
     9721 tttatatttg atgtgtgttc ccaacaagtg aataataaca aattttttgg gagctggatc
     9781 tgccagtact tcactaaacg gagccagtac gttagtatcg tactcacggg cactttgcgt
     9841 tctctgctga ttcatgtagt actgtttatc tgtttgccga gagaagactg tcagcattgt
     9901 gttgcgtgca gtcatcgttt gttgattggt gatccagaat gttttgtaac cggcctgttt
     9961 cattatattc atcagggaag gttgtgtcag gtagagttcg ggctgctgtt catcagcgaa
    10021 agtcaatacc tgctgtagta cttcgatggt atatggtctt gaggagacaa cattattaaa
    10081 cacaaacata ttttgttccg ttttgtataa ttcatctagt ccgggggttg tttttctggg
    10141 atatccatac agactcattc tcccacgctg ggttgattct cctatcacca gaaccagtgt
    10201 tcgtggggta tctccggaaa tatcgttaaa gtttttcaga gcggggagcg cattattttc
    10261 ctccagcaaa cggttcagag aattaagctg gagacggtac tgataatatc cggtaataaa
    10321 ttgccagggg gcagcgggct ccatacggga tgccaggcgt cccagagtat tgtcaatagt
    10381 cctgttttta acaacagtat ttattaatac agggtgcaga attaaaccgt agataataaa
    10441 taaaggtata atgtagcgcc agtatgatgg tatatatatc gggctaagac gagtccatag
    10501 cagaatagca atagctgtat atattgtcga tataaatagt atttttaaac taaaatactg
    10561 actaaaatat tcgctggctt cacttgtatt actttcaaac atgacgaaca gtacactctg
    10621 agagaattcc tgcccgtaga ttagataata gcaaagggca gcaagagatg tgccccataa
    10681 aaccacgccg attaatgctg ctgtaacctt tattctctct gggaaaagaa aaacagggat
    10741 taaccagaga gagctgaaaa gtagcgagtc acgaagtcca ttggtgccac tatatccagt
    10801 cagacaaatg ataatctgaa gtaaagtgga aaaataccag aaataaatga gagcccatcc
    10861 cagggctttc cagctaaaaa catgttcatg ttttgttaat gtcttctgca tattaataaa
    10921 cctgcacggg aatttactat tgtaattttt atatgagatg tattaaaaca tttcatacag
    10981 attttttgat aagcacatag tttcaatatt cactataatc gttttctgct gtgttgtgtt
    11041 tgatcctttt tcaaagaggc gctaatcttc cacttgccaa ggttagatgg tcagggtccg
    11101 ggttaaacaa aatgtttgac agggagcctt tccatatgct gtctttccag cgtggcggtc
    11161 cgtaatgtac taattttaag agaggtccgc aatcttccag tggatggttc atctgcctct
    11221 ctattaccgt taccatcgtc ttgcgtcctg tgctatcgcc tgtaacgaca tcagcgtcac
    11281 tggtattaac aaacttatag ttgaagccag aactgaaccg ccccggaaat cctggagact
    11341 aaactccctg agaaagaggt aaacaggatg actaaaaata ctcgtttttc ccccgaagtc
    11401 cgtcagcggg cgattcgtat ggttctggaa agtcaggatg aatatgactc acagtgggcg
    11461 gcaatttgtt ccattgcccc aaagattggc tgtacgccgg agac
//
LOCUS       DAZFJF010000130        11325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFJF010000130 DAZFJF010000000
VERSION     DAZFJF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.131"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..226
                     /locus_tag="ACHKAU_004020"
     CDS             <1..226
                     /locus_tag="ACHKAU_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5532989.1"
                     /translation="TTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            281..766
                     /locus_tag="ACHKAU_004021"
     CDS             281..766
                     /locus_tag="ACHKAU_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5532990.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            782..1258
                     /gene="radC"
                     /locus_tag="ACHKAU_004022"
     CDS             782..1258
                     /gene="radC"
                     /locus_tag="ACHKAU_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5532991.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1327..1548
                     /gene="yeeT"
                     /locus_tag="ACHKAU_004023"
     CDS             1327..1548
                     /gene="yeeT"
                     /locus_tag="ACHKAU_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5532992.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1711..2079
                     /locus_tag="ACHKAU_004024"
     CDS             1711..2079
                     /locus_tag="ACHKAU_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5532993.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            2169..2546
                     /locus_tag="ACHKAU_004025"
     CDS             2169..2546
                     /locus_tag="ACHKAU_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5532994.1"
                     /translation="MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKR"
     gene            2543..2692
                     /locus_tag="ACHKAU_004026"
                     /pseudo
     CDS             2543..2692
                     /locus_tag="ACHKAU_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            2792..2872
                     /locus_tag="ACHKAU_004027"
                     /pseudo
     CDS             2792..2872
                     /locus_tag="ACHKAU_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3688..4059
                     /locus_tag="ACHKAU_004028"
     CDS             3688..4059
                     /locus_tag="ACHKAU_004028"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5532995.1"
                     /translation="MLFLPPLQLSAPGSIGFKNRRQLAAWLGLVPRQCFSVGKNTLGS
                     ISKRDDVYIRTLLFHGAKTVSNARKGRQVQESWLGRPVSRRNKNVATVALNNKNARVL
                     WVLLTNDKEISPEEVMVAAYM"
     gene            4330..4695
                     /locus_tag="ACHKAU_004029"
     CDS             4330..4695
                     /locus_tag="ACHKAU_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO5532996.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(4796..5125)
                     /gene="tmaR"
                     /locus_tag="ACHKAU_004030"
     CDS             complement(4796..5125)
                     /gene="tmaR"
                     /locus_tag="ACHKAU_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO5532997.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5297..6355)
                     /gene="yeeA"
                     /locus_tag="ACHKAU_004031"
     CDS             complement(5297..6355)
                     /gene="yeeA"
                     /locus_tag="ACHKAU_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5532998.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(6553..7026)
                     /gene="sbmC"
                     /locus_tag="ACHKAU_004032"
     CDS             complement(6553..7026)
                     /gene="sbmC"
                     /locus_tag="ACHKAU_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO5532999.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7144..8310)
                     /gene="dacD"
                     /locus_tag="ACHKAU_004033"
     CDS             complement(7144..8310)
                     /gene="dacD"
                     /locus_tag="ACHKAU_004033"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO5533000.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            8519..9946
                     /gene="sbcB"
                     /locus_tag="ACHKAU_004034"
     CDS             8519..9946
                     /gene="sbcB"
                     /locus_tag="ACHKAU_004034"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO5533001.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDTAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(9989..10216)
                     /gene="tsuB"
                     /locus_tag="ACHKAU_004035"
     CDS             complement(9989..10216)
                     /gene="tsuB"
                     /locus_tag="ACHKAU_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO5533002.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10230..11288)
                     /gene="tsuA"
                     /locus_tag="ACHKAU_004036"
     CDS             complement(10230..11288)
                     /gene="tsuA"
                     /locus_tag="ACHKAU_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO5533003.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         3000 a         2937 c         2674 g         2714 t
ORIGIN      
        1 caccaccgcc gacattctga cgccacgacg ccgggaggat tacggtaagg acctgtggag
       61 tgcttatcag accattcagg agaatatgct gaaaggcgga atttcaggtc gcagcgctaa
      121 aggaaaacgt atccataccc gtgccattca cagcattgac actgacatta agctcaaccg
      181 cgcattgtgg gtgatggcgg aaaccctgct ggagagcctg cgctgattca gccattaccc
      241 tgaaaaccac gcatttattt gtcctttaag gagtcatttc atgaaaacca tttcacataa
      301 ttccaccaca tcttctgttt ccgtaaccgc tgcatcaggg aatgaccagc ctcagttggt
      361 tgccaccata gtccctgatg aacagcgcat cagcttctgg ccgcagcatt ttggcctcat
      421 tccacagtgg gtgaccctgg agccccgtgt tttcggctgg atggaccgtc tgtgcgaaga
      481 ctactgcggt ggtatctgga atctgtacac tctgaacaac ggcggggcat ttatggcccc
      541 cgaaccggat gacgatgatg acgaaacatg ggtactgttc aatgccatga acggcaaccg
      601 cgctgaaatg agcccggaag ccgctggcat tgctgcctgt ctgatgacgt acagtcatca
      661 tgcctgtcgt acggagtgtt atgccatgac ggtccattat taccggctgc gggattacgc
      721 cctgcagcat ccggaatgca gcgccattat gcgcatcatc gactgaatgg aggcagggac
      781 aatgcagcag ctttcctttc tgcccggaga aatgacgccc ggcgagcgca gtctcattct
      841 gcgggctctg aaaaccctgg accgccatct tcatgaaccc ggtgtggcct tcacctccac
      901 ccgtgcggca cgggaatggc tgattctgaa catggcggga ctggagcgtg aagaattccg
      961 ggtgctgtat ctgaataacc agaatcagct gattgccggt gaaaccctct tcaccggcac
     1021 catcaaccgc acggaagtcc atccccggga agtgattaaa cgtgccctgt atcacaatgc
     1081 cgcagccgtg gtgctggcac acaatcaccc gtccggtgaa gtcaccccca gtaaggcaga
     1141 ccgccttatc acggaacgtc tggtacaggc gctggccctg gttgatatcc gggtgccgga
     1201 ccatctgata gtcggtggca gccaggtttt ctcctttgcg gaacacggtc tgctttaacc
     1261 cgtcaccgtc acaaccacat cacacctgtt ttcactttta tcttctgtct tcagaggtat
     1321 cacactatga aaatcatcac ccgtggtgaa gccatgcgta ttcaccaaca acatccgaca
     1381 tcccgtcttt ttccgttctg taccggcaaa taccgctggc acggcagtgc tgaagcgtat
     1441 accggtcgtg aagtgcagga tattcccggt gtgctggccg tgtttgcaga acgccgtaag
     1501 gacagttttg gtccgtatgt ccggctgatg agcgtcaccc tgaactgatg ttccgtcacc
     1561 cggaaaacga gtacgactgg cagagctcag acgccgctgt ggatgaggtc ctgctcatca
     1621 acgaatacac ctttaccgaa gccggtctgc gcgccggctg acccgtcatc gttaaactat
     1681 cattcaccga ttttctgtga ggattttatc gtgtcagaca cactttccgg gacaacgcat
     1741 cccgatgaca atgacgacca tccctggtgg ggactaccct gcacagtgac gccctgtttc
     1801 ggcgcccgtc tggtgcagga gggtaaccgg ttgcattacc ttgccgaccg tgccggtatc
     1861 agaggccggt tcagagatgc ggatgcgtac cctctggacc aggcctttcc gctgctgatg
     1921 aaacaactga aattaatgct caccagtggt gaactgaatc cccgccatca gcataccgtg
     1981 acactgtatg cgaaggggct gacctgtgaa gccgacaccc tcggctcctg tggttacgtt
     2041 tatctggctg tttatccgac acctgaaacg aaaaagtaaa ccaccggaat aaccctctac
     2101 tccggcctgg tgttttcacc acgccactgt cccatttttt atctctgcat atcaggaaaa
     2161 tcttcagtat gaaaacatta cccgacacgc atgtacggga ggtatcgtgc tgcccgtctc
     2221 tcgtcaccat ctggcagaca ctgctcaccc gactgctgga ccagcactac ggccttacgc
     2281 tgaatgacac accgttcgct gatgaacgtg tgattgaaca gcatattgag gcaggcattt
     2341 cactgtgtga cgcggtgaac tttctcgttg aaaaatacgc gctggtacgt accgaccagt
     2401 cgggattcag cgcctgtacc cactctcagt taataaacag catcgatatc ctccgggctc
     2461 gccgggcaac aggcctgatg acccgcgaca attacagaac ggtaaataac attaccctgg
     2521 ggaagcatcc ggaggcgaaa cgatgaaact ggccctgacg ctggaagccg acagcgttaa
     2581 cgtgcaggcg ctgaacatgg ggcgcattgt cgttgacgtc gatggtgtta atctcgctga
     2641 actgattaac aaggtcgctg aaaacggtga ttcactccgc gtggtggatg aacgtgactc
     2701 taccgaaaca ccggcaactt atgccagccc tcaccagttg ctgtaagaca atgcagtgat
     2761 acagggtata gtgccattaa ccacagaaac agcactgttg tcctgtatta agcaggcaag
     2821 agaagatgtc cttaccctgc gccatctgca gcttctgcac cagaaccggt gaatctcact
     2881 caacaggctt catttcccct gacgccagcc tgaatacagc tggcgttttc atttataaac
     2941 agaaaggaaa accgataata atggaatgga cgcccctacc atgaagcaac ggtaggatat
     3001 tactgataaa ccttccagtg gaggtgcgtt atggccgttt taacgattag cattgacctg
     3061 acaaaaaatg aattccaaat ccacgatctg ggtaggaaca gaaaaatcta agctcagaaa
     3121 acggattaaa cgttccctga tgcccgtatt cttcgcgaat atgtcttcct gtcggattgg
     3181 gatggaggtc tgcgccggag cgcgtcatta gtgagaatgc tgtctactta ggggcataat
     3241 gtcagactaa tgcccgcgat ttgtaaagcc atatgtgaaa acaaacaaga atgatatggc
     3301 tgctgccgag gccatctgtg aagccgttac ccgacctaat atgcggtttg tcagcatcaa
     3361 gacgcctgag cagtagtcat tactgtcttt tcatcgtgcc caaacgggat ttgttaaatc
     3421 aagggctgcg cagataaacc agatcagaga attactggct gaatttggca tcgttttccc
     3481 gcccgacatt gtagcattca gtcgtcatgt ccctgccctg cctgaagacg cggaaaactg
     3541 tttaacaatg ccgttcagac agctgtcgtc ctatctctaa tgaaaatgtg aaacaactga
     3601 gcgtacacat tgaaaccata gaagccgtga gcaacgaaca cgtcgggaac gacgaactgt
     3661 gtaggaaaat tgccaccatt tcggggattg ctattcttac caccactgca attgtcagca
     3721 ccgggtagta tcggcttcaa aaaccggcgt cagcttgctg catggcttgg actggttccc
     3781 cgacagtgct tcagcgtagg taaaaacacg ctggggtcta tcagtaaacg ggacgatgtc
     3841 tatatcagaa cgctactgtt ccatggtgcg aagacagtgt caaacgccag aaagggcaga
     3901 caggtccagg aaagttggct gggcagacca gtctcgcggc ggaataaaaa tgttgccacc
     3961 gtagcgctga acaacaaaaa tgccagagtg ctctgggtat tgctaaccaa cgataaagag
     4021 atttcaccag aagaagtgat ggtagctgca tatatgtaaa tgaaactact tctgtctgtt
     4081 gaaaaataaa accaacctac tgattgctaa ggataaacaa gtattaaagg ccagactgat
     4141 aagaccgcga ctaagtgaac ctgtatctga catggcatag cttcgctaat gtgtcatcat
     4201 gattaggact aaatctgtga attcctttag gaagcacgga cacaggccaa tctgagtcca
     4261 tatgtatgat tggaatctta cacagatgac tttttgacac ggggacttgg caaacagggg
     4321 cgaccatgta tgtcagatat gacaatctca cttccgaaag aagtcaacgg gcacgttatc
     4381 tgttcaacca gaatccgaca cattctggct atacgaaaga gcatacttat gcaaatctat
     4441 atgcttttcc ggcaacaagc aggaatctca gcgctgaggc aggaattaga ggtaaaaacg
     4501 ccctaccggg ccatgaatca cccggtcatt ggagtagtaa ccaaagcaga tttagccagc
     4561 atggaacaca tatctttggt gaaatgttgg ttacgggaag ctggagcgca caacgtgtta
     4621 gtaaccagtg cggttaataa caatggagtt acagaacttt ttgccttgct gcatacagaa
     4681 gaaggctgtt gctaatttcg ataggttctc agatgagaat gatgacggtg atacacagat
     4741 aagaatcaat gagcaatgag ggttgccggg caaccctcat tgaataaaac aggaattact
     4801 tcgcttcgcc gtttttcatt tcgcccatag ctttcagctt tttggagata tcgcggcgtt
     4861 ctttggagag ctcggcattt ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt
     4921 tcatgctggc gatgatgccc tggattgctt caacgctcat cccgggcttg atgtaatcgc
     4981 tcaggttgtc cagcaacagg acgcgcttct ggttgtcacg gatctttttc tcaacgtcct
     5041 gaatttcacg ttgcagtttg ttcttacgac ggaacagacg aacaaattcc agtacgtcct
     5101 ggaatgaagg cttggtagtt tccattttta tacccctgat aatgtgagag tcggattcgt
     5161 ttaagcaacc gctattcgtt agggccaaca ttactcatag ctgccgcaga tgacaatgct
     5221 tttatccctt ttcactatca taccctttat ccttgctgaa tcgaagcagc agcaagatga
     5281 ttctgaagtt caggaattat ttgcgcaagg cccggcaaat cagactcgat agcaactcaa
     5341 gctgatgtgc cgtttccatg ttcagccaca cataaccata gattggtgtt tccacaacct
     5401 tcaggtcatg gtggttattc agcaactgac gcagctcttc cacagcatcg atcaattttt
     5461 ccgtattggc aaaaaccggc tgcggattac cttcgtacag cgcatgaaca aggctcagca
     5521 gtatttgctg catcatgtgc tgggtatcac gaagtttttg cgcgttcaat aacacgaaat
     5581 ggctggggcg cgtggcccag tatgcattga tttgcaactc cagcatacaa accagattgc
     5641 ggttaatggt ctggatacct tcatatatcg attttggaat acgagtttct ttgctggcgg
     5701 gcgcaatcag cccacgcatt ttcacggcat cggtcaggag tttttgtaga tggctttcca
     5761 gacgtgggcg ttcgagtaag ttcggtgaga atgcagattg ataaacccga ttatactcgg
     5821 tcagactttt cgccagttga atgcgccagt ggatgaacgc ccgttgtggc cagataccgg
     5881 taaacaacat tgccagtaaa gagccgagga tcacatcgcc gcttcgccat aacgccgtat
     5941 caatttcacc tgtcggggaa cccacaacaa ttgccagcgt caccccaatc aataatcctt
     6001 gatacggttt cttgcccagc gccagccaac cgcaaagaaa catagccgcc gcgcaccaga
     6061 ctaacatcag cggtaacgaa attaactcca gttgcagagc gataagacct aaaatcgaac
     6121 ccaacaccgt accgccaata cgttcgaagg cgcgagggac aacgttaccc cagaacgaga
     6181 ttggccccat aatcaccacc atggtgacca gcggccaggt gctttccggg atagtaaaca
     6241 ggcggataat gagaaaagtg agcaggaatg ccagcgcgac ccgagtacca tgcacaatgc
     6301 ggtaatggcg gtatacccgg atttcaaacg ggcttaatga cttatcggca cgcacgcaga
     6361 aactccgtaa tgaagattaa aagtgctcat aactcatctg tcggccagaa ggcccgcctc
     6421 tgaattatcc gacggacaaa ccgggaacag tgtaatttcc cggcaggagc gaaaaaattg
     6481 aaaggcgcat cttactcttt tcgctttcaa aaaaagagtg gtcatcgcgt taacacaccg
     6541 ccctgagatg aattagtgat gttttggctg caccgcaaca tacatttcga tatcccagta
     6601 cccatcttcc gcgccattgt tcaaataaac ctcgaagcag ggctttggta acatttcata
     6661 agcactgtcc tgcaagaggc tattaaagaa ctgataccag ggtttagcaa aatcatcacc
     6721 gactacacga gccaccgcaa ccgcatactg accacctgaa atttctgtca gaatgacgcc
     6781 ctcactgttt tcggggagcg taaagttatt cggcaccgtc acgacgatgt cgcagcgtaa
     6841 tttttcggcg ggtgtttcat ctggattgtc gtaaaagaca gcaacccact ccttcggcac
     6901 aatatttttg ctatctaccc acatcatcaa ctgctcaaag cctttcttta ccgtctgttc
     6961 ccacgggcca acgagatgga aacctgcaac ggtacgttta tcttcctgct taatctcgta
     7021 gttcatgacg tcccattaac actgttttta tatacagtat aattgcgata ttaaaaccac
     7081 aaggaatgag tgttgattat gcgagcagac tcgcactcct gccagtctgc tgcaaaagaa
     7141 aggtcaggcc ttatggtgga aataatcact caggcgagaa aacatgctgc cttccccgac
     7201 agattccagg gtaaccagcg gccagtgcgc cacctgttta tcacggtcgt aaagttcaat
     7261 ttcccctacc cgctgatggg cgctaattgg cgcggtgagc tctttaccat caagggtata
     7321 tttggctttg atatgtggaa tttcggcttt cggtagcacc atccagaact cttgttccgt
     7381 tcccaggtcg atattttctt tatcgccata ccagatgcgt tccgtaccga cctttttccc
     7441 acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc caacgcagta attttcttgc
     7501 ctcttcctca cgaccttttg cactgtcagc ccccattacc actgcaatga gacgacgctg
     7561 cccatcgaca gccgaagcaa tgagattaaa cccggcacca gaagtatgac ccgttttcag
     7621 gccgtcaaca ttcatggttt tatcccacaa taacccgtta cggttttgct gggtgatacc
     7681 gttccaggtg agacttttct cactgtacat atgataaaac tcgggctcgt cgtggatgat
     7741 agcgcgagaa agcacagcta aatcataagc cgagctatgc tgacctggtg catccagacc
     7801 atgcactgtt tcaaaatgcg tatccttgag atgcagcttc tcggcatagt tgttcatcat
     7861 ttcaacaaac tgccgttgcc caccggcaat atagtcagcc agtgcaacac aagcgtcatt
     7921 tccggaatcc acaattaaac cacggcttaa atcacgtacc gatacgcgat cgccctcttt
     7981 caaaaacatc agtgaagaac cgacaaacac tggattatct ttcgcccacg catcgcgccc
     8041 cacggtgaca atatcgtctg gcgtaatgcg atgactatcg atagtgcgat ccacgacata
     8101 gcccgtcatc agctttgtca ggctggcggg attgcgctgt tgatgctcat tacccgcggt
     8161 gaggatctga ccggtggtgt aatccatcag tacccaggag ccggcatgaa tctctggagg
     8221 ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac gataagttaa aaatgaacaa
     8281 agaagcagca ataataagac ggcgtttcaa cagcaaaccc tcaggagttt caaatagctg
     8341 ttctttttac ggaaatactt atgaactggc tggaataaag tgcaagaaaa tgtgactacc
     8401 ctctcatttt tatctgacat gatctgttgt cactcgctgt caaattgtcg cgctaaagct
     8461 gattagcacg gtgatatttg atactctggc agacagcaga aataacggat ttaacctaat
     8521 gatgaatgac ggtaagcaac aatctacctt tttgtttcac gattacgaaa cctttggcac
     8581 gcaccccgcg ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa
     8641 tgtcatcggc gaacccgaag tcttttactg caagcccgcg gatgactatt taccccagcc
     8701 tggagcagta ttaattaccg gtattacccc gcaggagaca cgagcgaaag gagaaaacga
     8761 agccgcgttt gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg
     8821 ctacaacaat gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta
     8881 cgatccttac gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat
     8941 gcgtgcctgt tatgccctgc gcccggaagg aataaattgg cctgaaaatg atgacggtct
     9001 accgagcttt cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca
     9061 cgatgcgatg gctgatgtgt acgccactat tgcgatggca aaactggtaa aaacgcgtca
     9121 gccacgcctg tttgattatc tctttaccca tcgtaataaa cacaaactaa tggcgttgat
     9181 tgatgttcct cagatgaaac ccctggttca cgtttccgga atgtttggtg catggcgcgg
     9241 caatacaagc tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat
     9301 ggtggatttg gcaggagaca tttcgccatt actggagctg gatagcgaca cattgcgcga
     9361 gcgtttatat accgcaaaag ccgatcttgg cgataccgcc gccgttccgg ttaagctggt
     9421 acatatcaat aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga
     9481 ccgactggga attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc
     9541 gcaagtgcgt gaaaaagtgg tagcgatatt cgtggaagcc gaaccgttta caccttcaga
     9601 taatgtggat gcacagcttt ataacggctt tttcagtgac gccgatcgtg cagcaatgaa
     9661 aattgtgctg gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa
     9721 acgaattgaa aagctgttgt tcaattatcg ggcgcgcaac ttcccgggga cgctggatta
     9781 tgccgagcag caacgctggc tggagcaccg ccgccaggtc ttcacgccag agtttttaca
     9841 aggctatgca gaagagattc agatgctggc gcagcagtat gccgacgata aagaaaaagt
     9901 ggcgctgtta aaagcgcttt ggcagtacgc ggaagagatt gtctaatgtg aagcctccgc
     9961 cgctggtacg gcggaggaca attttgagtc aggctttttg aacggtgatg ctccacgctg
    10021 catcgccaat ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga
    10081 ttgcttcagt tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt
    10141 ctgccagtgc tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca
    10201 gctttttaat cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgtgcc
    10261 ttacgctgcg gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat
    10321 acaaggccaa tccagccctg ccaggtcatc attgcagtca tgaccagtcc attaccgata
    10381 gagcaaccac ctgcaatgct ggcaccgaag cccatcagta caccacctaa cccgctacgt
    10441 aatgttgttt gtgcatcagc agcgcgaacg cggaactcac ggctcgcttt ggctgcaata
    10501 aatgacccca cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca
    10561 cccgcgacca gaaattgcag aatattggcc gttggggaag tgattcctaa cccaaacatg
    10621 cgtccggttg cttcactcag gggccaagct aaaagcgcaa tcaaaccgat gagtacagca
    10681 gtgacaaagg gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc
    10741 ggtaaggtcg cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt
    10801 atcaccagca aaacggcaac caacggccac acagacaaat taaaagtatc agcaatagag
    10861 ttatgttcag tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc
    10921 atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca
    10981 cccgcgcgat accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa
    11041 agatacccac ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg
    11101 cccgcctgaa ttaacgcaaa aacccccacg ctttgtaccg aaattgcaat cagcaaggcg
    11161 taaaacatgc gattattttt cgcgatatac atatcgcgaa aaccacctgt caggcaaaaa
    11221 cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt
    11281 gaaaacataa attaattaac cagatgaatg ttaatgggga aatta
//
LOCUS       DAZFJF010000131        11276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFJF010000131 DAZFJF010000000
VERSION     DAZFJF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.132"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..1889)
                     /gene="glmS"
                     /locus_tag="ACHKAU_004037"
     CDS             complement(60..1889)
                     /gene="glmS"
                     /locus_tag="ACHKAU_004037"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO5533004.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2051..3421)
                     /gene="glmU"
                     /locus_tag="ACHKAU_004038"
     CDS             complement(2051..3421)
                     /gene="glmU"
                     /locus_tag="ACHKAU_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO5533005.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3772..4191)
                     /gene="atpC"
                     /locus_tag="ACHKAU_004039"
     CDS             complement(3772..4191)
                     /gene="atpC"
                     /locus_tag="ACHKAU_004039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO5533006.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4212..5594)
                     /gene="atpD"
                     /locus_tag="ACHKAU_004040"
     CDS             complement(4212..5594)
                     /gene="atpD"
                     /locus_tag="ACHKAU_004040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO5533007.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5621..6484)
                     /gene="atpG"
                     /locus_tag="ACHKAU_004041"
     CDS             complement(5621..6484)
                     /gene="atpG"
                     /locus_tag="ACHKAU_004041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO5533008.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6535..8076)
                     /gene="atpA"
                     /locus_tag="ACHKAU_004042"
     CDS             complement(6535..8076)
                     /gene="atpA"
                     /locus_tag="ACHKAU_004042"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO5533009.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8089..8622)
                     /gene="atpH"
                     /locus_tag="ACHKAU_004043"
     CDS             complement(8089..8622)
                     /gene="atpH"
                     /locus_tag="ACHKAU_004043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO5533010.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8637..9107)
                     /gene="atpF"
                     /locus_tag="ACHKAU_004044"
     CDS             complement(8637..9107)
                     /gene="atpF"
                     /locus_tag="ACHKAU_004044"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO5533011.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9169..9408)
                     /gene="atpE"
                     /locus_tag="ACHKAU_004045"
     CDS             complement(9169..9408)
                     /gene="atpE"
                     /locus_tag="ACHKAU_004045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO5533012.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9455..10270)
                     /gene="atpB"
                     /locus_tag="ACHKAU_004046"
     CDS             complement(9455..10270)
                     /gene="atpB"
                     /locus_tag="ACHKAU_004046"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO5533013.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10279..10659)
                     /gene="atpI"
                     /locus_tag="ACHKAU_004047"
     CDS             complement(10279..10659)
                     /gene="atpI"
                     /locus_tag="ACHKAU_004047"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO5533014.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2712 a         3055 c         2770 g         2739 t
ORIGIN      
        1 tgatgtcagt tataaaaaag ggccaccggt cggcagccct tttttatcta tgatagtgat
       61 tactcaaccg taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc
      121 agcgcgacat ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc
      181 tcttccacat gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc
      241 tgatcggcga agacatacaa ctgaccgcca cgcgcgcgaa cttcttcaat gttggatttc
      301 agtttttcca acagttcgtt gttcggtgca acgacgataa ccggcatatc cgcgtcaatc
      361 agcgccagcg ggccgtgttt cagctcgcct gcagcgtagg cttcagcgtg aatgtaagag
      421 atctctttca gcttcaatgc gccttccagc gcgattgggt actgatcgcc acggcccagg
      481 aacagcgcgt gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga
      541 gacagcatct gctcaatacg gctcggcagc gcctgcagac catgcacaat gtcatgttca
      601 atggaggcat ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt
      661 aactgagtgg tgaatgcttt agtggatgcc acgccgattt ccgtacccgc gttggtcatc
      721 agcgccagat cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa
      781 ccaaggtaac ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca
      841 gactgtgaca aggtgatcat caggctgtta cgacgcacgg cagatttgcg atagcggaat
      901 tcagaggcga tttcgacgtc gcacggaata cctgccagcg attcaaacca gtaacgggaa
      961 accataccgg agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac
     1021 agcagttcgt cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc
     1081 ccggtaaggg tgtttttgat cgcgttcggc tgttcgtaga tctctttctg catgtagtga
     1141 cggtaaatac ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt
     1201 acttccgcgc cagttttatc gaagatgttt accgaacggc gagtaatttc cgcaatatcg
     1261 ccctcttcaa ggaagataaa gcgacgggtc accggcagca gcgccagctg gtcagaagcg
     1321 ataaagtttt cgcccatacc cagaccaatc accagcggac taccagaacg tgccgccagc
     1381 agggtatccg ggtgacggga gtccatgatc actgtaccgt acgcaccacg cagttgcggg
     1441 atagcacgca gaacagcctc acgcagagta ccgccctgtt tcagctccca gttcaccaga
     1501 tgggcaatta cttcggtgtc tgtttcagaa acgaaggtat agccacgcgc ttttaactct
     1561 tcgcgcagcg gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa
     1621 acatgcggat gcgcattcac ttctgaaggt tcaccgtggg tcgcccagcg agtatgagca
     1681 atacccgtac cgccatgcag aggatgttct tccgctgcct gagccagcat ctggacttta
     1741 ccgaggcgac gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag
     1801 tcatatccgc ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt
     1861 tgcgcgatcg cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg
     1921 tcaacctgta tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt
     1981 atagttactg cttgtgggag ggagattatg ttatctcctc atcccatgtg accggattac
     2041 ccggccagaa tcactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca
     2101 cacggctgat agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa
     2161 tggtcgcgcc tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca
     2221 catcgtcgcc gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc
     2281 ccgcgccgat gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgaccag
     2341 ctttcgagcc tttacccaga cgcgcttttt tcatctcaac aaagttaccg acgtgtgcac
     2401 cttccagcaa ctcagcacca ggacgcagtc gggcaaacgg gccaatagta caggccgctg
     2461 ccagattcgc gtcttccaca acggtatacg gactgatttc gcaatcatca ccaatcacgc
     2521 tgtttttaat cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct
     2581 cgatgataac gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcaaat
     2641 caaaacgcgc cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg
     2701 actggtaaac gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc
     2761 ttaaacgttg cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa
     2821 tgtcggtgat gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt
     2881 tcatatctgc gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct
     2941 cgtcggtggc atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac
     3001 gtccataacc ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt
     3061 tagcatcacg caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca
     3121 ttaaaatgtc ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac
     3181 ccagctgctc tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca
     3241 gatcgccgcc gtgaccgtac accaggtgaa cgtgcgctgc gcctaattca ttcgcagcat
     3301 caatgacatg ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg
     3361 aatacatgcg cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca
     3421 tacgcgtcct gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca
     3481 tttttttcat cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga
     3541 cgaaagtgcc attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc
     3601 ttcatttaag cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa
     3661 acaggggtgt tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta
     3721 aaaaaaagcc agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc
     3781 ttttttggtc aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc
     3841 ctgagcgtaa tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc
     3901 ttccatggct cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt
     3961 cacgttgcca ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc
     4021 gtgctgtttc acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc
     4081 agggtagatc cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga
     4141 gaacatttgt tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc
     4201 cgattaaggc gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg
     4261 tagaacgcct gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg
     4321 atggtgtctt tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag
     4381 aacggctggg acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct
     4441 tcagacagtt catccatacc caggatggcg atgatgtctt tcagttcctg ataacgttgc
     4501 aggatggact gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc
     4561 agctgacggc tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc
     4621 tgacggctca gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacggatca
     4681 gtcaagtcat ccgcaggtac gtataccgcc tgtacggagg tgatagaacc agttttggtg
     4741 gaggtgatac gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc
     4801 gctgaaggca tacggcccag cagtgcggat acttccgtac cggccagggt gtaacgatag
     4861 atgttgtcaa cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc
     4921 agaccggtca gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc
     4981 agggatactt tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca
     5041 cgagtacgtt cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta
     5101 cgaatgagct ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact
     5161 ttaccgccct tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt
     5221 tcctgagagt ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct
     5281 tcttcaccga tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc
     5341 agagtcgctt tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg
     5401 cgacgcagac cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc
     5461 tgctgaactt ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg
     5521 cgcggtacgg catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc
     5581 tttccagtag ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg
     5641 agacgatctc ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact
     5701 gcagctcttt aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg
     5761 cggcctgctc gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac
     5821 gacgcagcag ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt
     5881 tatgtttcag atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa
     5941 cctgagacat ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt
     6001 cgtcgtaggc ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat
     6061 cccccatgcc ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc
     6121 ctttcgagcc gatcattgcg aggtcgcatt gaacgccttt gtcggtccag gtcttcattt
     6181 ccgccagcag ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg
     6241 acaccaccag gtagcccacg cgtttaacgt cgcgttcttc caggtatggg tgcttatatt
     6301 ccagattacc gctcgcaagg tgaccaatca ctttgcgcat ggtatctgca taaggacggc
     6361 tggccgccat gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt
     6421 tagtgatctt ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg
     6481 ccatgagctt ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca
     6541 ggattgggtt gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt
     6601 gtagccaccg gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc
     6661 cagcagagcg gcttcgaagc tgccgatttt cgacagttca acatccgcca ggtaaccacg
     6721 ttctgctgcg aacagaacca gagactgttg cgctacggac atcggcgcat actgtttctg
     6781 tttcagcagt tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag
     6841 gtcggatgca aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat
     6901 accaccggac agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga
     6961 aatacccggg ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat
     7021 ctgaccatcg gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg
     7081 agtttcaata atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt
     7141 gaaggcttca acgtattcgg cgttaacacg cgcagcacgc tccagcagac gagagtggag
     7201 gtagaatacg tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat
     7261 ctgacggtaa gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc
     7321 gcggtcacgg aagtattcgc ccattgcgca accggcatac ggagccagat attgcagtgc
     7381 agcggattca gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc
     7441 cagtttacgt accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca
     7501 tttgataccg gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttaccggt
     7561 ctgacggtca ccgatgatca attcacgctg accacgacca attgggatca tggagtcaac
     7621 ggctttataa ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc
     7681 gattgcttct acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc
     7741 acccagagtg ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg
     7801 gccagtacac ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc
     7861 acctacagag tcgcgctcga ggttcagtgc gatagcgtaa cggttacccg gcagggagat
     7921 catttcaccc tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga
     7981 aacaatagta ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt
     8041 gatcagttcg ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa
     8101 gacgtctgca aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc
     8161 tcggatgata acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg
     8221 tgacagacgt ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc
     8281 agcggaaatg acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg
     8341 ctccagaaca tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt
     8401 ctgaccgttt tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc
     8461 tggcgcaagc gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc
     8521 aaacgccagc atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc
     8581 tgctttggcg taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac
     8641 agttcagcga caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg
     8701 atgatcttct cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg
     8761 gcacgtttac gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc
     8821 tgttctgcct cagctttcgc ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg
     8881 atgattacct gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca
     8941 aggtcaaggt ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga
     9001 cgtttttcga tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac
     9061 aggacaaacg cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc
     9121 tatttagtta acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc
     9181 gaacatcacg tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat
     9241 aacgataaag aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc
     9301 caggaattta cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat
     9361 catcacagcg gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag
     9421 tttgtttcag ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca
     9481 gatagacgat cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga
     9541 aaatggccca cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca
     9601 gaatgaaaat cagctcaccg gcatacatgt taccgaacag tcgcaaacca agtgaaactg
     9661 gtttggacag caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat
     9721 tgaacggctg cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt
     9781 agaacagaat caggataaat acgcccaatg ccatagacag cgttacgttc acgtccgcag
     9841 acggaaccac acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt
     9901 cgataggcag taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca
     9961 gcggagcaat cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc
    10021 cgatcaccag ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg
    10081 ctacgctacg gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt
    10141 caatattgat tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac
    10201 gcaggtccag ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt
    10261 ctgaagccat gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac
    10321 caccagcacc aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa
    10381 cgccaccacc agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt
    10441 ccaggccacc cggcctttcg ctggtgtatg cgcctggtga cgccaggcaa atatcataaa
    10501 caaaacgtta ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg
    10561 gtctttgagg ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag
    10621 cttccgagca acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc
    10681 cttcgaggta tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa
    10741 aagcggtcaa attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga
    10801 ataaatgttt aaatattttt ccagtgcata caattgcgac ttttctgcta accctgttcg
    10861 atcatgaaaa actgtaaata acgcgtaaac actggtgata aagcgtgctt cagatcacat
    10921 attgcgcatg ttcgcgcaca gcatatttat ttacttggca aatgatgcct ttgcaagttt
    10981 atgatatttc agtctaaaaa cagatactgt tttaataaat gacatttaca caacaaaaac
    11041 cacccattga catttttaat aatgttttaa cagccaatga tggttcttag cgccgatttt
    11101 tagcagactg atattttcac taatgactta ttttctgctt accaaaaaaa gccacgttat
    11161 cttgttgatg caaaagagtg aacgtggcgt taaatgtaac cagttatatc agtagaaaac
    11221 ctggttgttg ttaacagtct aaccggtcaa ttttttatga tttttttgat aaaaat
//
LOCUS       DAZFJF010000132        11202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFJF010000132 DAZFJF010000000
VERSION     DAZFJF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.133"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..297)
                     /gene="espW"
                     /locus_tag="ACHKAU_004048"
     CDS             complement(<1..297)
                     /gene="espW"
                     /locus_tag="ACHKAU_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO5533015.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNSVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRI"
     gene            complement(372..1022)
                     /locus_tag="ACHKAU_004049"
     CDS             complement(372..1022)
                     /locus_tag="ACHKAU_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5533016.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            1205..1795
                     /locus_tag="ACHKAU_004050"
     CDS             1205..1795
                     /locus_tag="ACHKAU_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HFO5533017.1"
                     /translation="MPMNTTGTSFSSFGISCHRENSFRNSFRGKNDEVIKCSMGERTI
                     RFSVRKFSGNILDTVSRQSTKDINGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQEYQLTLDVKAAQSSINHVIMGNASFGKKIDALCDSMSRDVKNRTADSI
                     ANLLADKFYQKHIEPDIDIVKLRNEIPDYLRCAIQA"
     gene            complement(1821..1904)
                     /locus_tag="ACHKAU_004051"
                     /pseudo
     CDS             complement(1821..1904)
                     /locus_tag="ACHKAU_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(2069..2932)
                     /gene="potB"
                     /locus_tag="ACHKAU_004052"
     CDS             complement(2069..2932)
                     /gene="potB"
                     /locus_tag="ACHKAU_004052"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO5533018.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
     gene            complement(2916..4052)
                     /gene="potA"
                     /locus_tag="ACHKAU_004053"
     CDS             complement(2916..4052)
                     /gene="potA"
                     /locus_tag="ACHKAU_004053"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO5533019.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            4302..5531
                     /gene="pepT"
                     /locus_tag="ACHKAU_004054"
     CDS             4302..5531
                     /gene="pepT"
                     /locus_tag="ACHKAU_004054"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO5533020.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(5677..6798)
                     /gene="roxA"
                     /locus_tag="ACHKAU_004055"
                     /gene_synonym="ycfD"
     CDS             complement(5677..6798)
                     /gene="roxA"
                     /locus_tag="ACHKAU_004055"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO5533021.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            7047..8276
                     /locus_tag="ACHKAU_004056"
     CDS             7047..8276
                     /locus_tag="ACHKAU_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5533022.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLSDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWMERLGI
                     LAGTHENVTALPAARRK"
     gene            8642..8830
                     /locus_tag="ACHKAU_004057"
     CDS             8642..8830
                     /locus_tag="ACHKAU_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5533023.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            8888..9388
                     /locus_tag="ACHKAU_004058"
                     /pseudo
     CDS             8888..9388
                     /locus_tag="ACHKAU_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9331..9510)
                     /locus_tag="ACHKAU_004059"
     CDS             complement(9331..9510)
                     /locus_tag="ACHKAU_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5533024.1"
                     /translation="MATKAVNAKSQTVAARVPHEVMNNVEAVKMPGESTGQFVTAALK
                     REVEYRQRRKAKEPE"
     gene            9710..9916
                     /locus_tag="ACHKAU_004060"
     CDS             9710..9916
                     /locus_tag="ACHKAU_004060"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5533025.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQEVCHVQ"
     gene            9906..10370
                     /locus_tag="ACHKAU_004061"
     CDS             9906..10370
                     /locus_tag="ACHKAU_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533026.1"
                     /translation="MCNNIRPDAAAEAIITLMHALIDISVIADMAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            10363..10596
                     /locus_tag="ACHKAU_004062"
     CDS             10363..10596
                     /locus_tag="ACHKAU_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533027.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLDPVIMELQEIDLLHDRYKEQHTGE"
     gene            10602..10901
                     /locus_tag="ACHKAU_004063"
     CDS             10602..10901
                     /locus_tag="ACHKAU_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5533028.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            10898..>11202
                     /locus_tag="ACHKAU_004064"
     CDS             10898..>11202
                     /locus_tag="ACHKAU_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5533029.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANY"
BASE COUNT         3064 a         2537 c         2741 g         2860 t
ORIGIN      
        1 tatgcgggag cgtttatcat cactgtttat ggcatagtat tgggctccgc tcctgcttcc
       61 cattcctgga gtccctgcaa taataaatga gtgacttctt gtatgtctgc cattgagtag
      121 agcaagggga gttttagctg tagccaacca tacaggaacg gattttaaag atataccata
      181 ttctttatga acaattcttc tggagacgga gttcgtcatt gttgtttcat ttgaaagttg
      241 ttgatgttca ctgaacagat ggtaacatga tgatacaact gatgatattt tgggcataaa
      301 taatctcctc gctatatgtt tgttatatgg tgattaactt attgtatgct tcttatattg
      361 tttttttatt tttaaatact gttttgttga agggggtata tgggtaatgc gtaaaaacat
      421 tgttttgtta tattaaaatg acacttgtct ttgcttataa tcatttctgg agcaaagggg
      481 tcacggctga gggggtggag agcgttacgc aggataagtt cagtcaatgc ttcctgatca
      541 tataatgcac atatttctga atttcgagca tttctgacaa aaacacctgt ctcaggaata
      601 tgtagcgtta tcggacaatt aagatgctcc tcagtacagg aaaagtcttg taaactaacg
      661 ggaaatgcat tcgaatttat tcgatctagt agtgttttta tgacgctttc ctgcacaagc
      721 ggggcagctt gcctctgctc cagatataag ctgtatgcct gcagaaaagt gttacctccg
      781 ttaagttgtc ttgccaggct gtctgcgaga tgatgagact ctcttgcccc aagatggtcc
      841 caaagtccac caggaatagg ctctacacta aaaccatcca taaactggat ataatgaagc
      901 ctcatattac ggcctcctat agtaatagtg tcattctgat tgcttcttgc aatatgtcgt
      961 aaagcctcca gctcattttc cggcaataca cttccattag aaaaatttaa tatgactggc
     1021 atattattat ccttttcaac ccattttgtg aactatattg gtaacaagag tcaacatacc
     1081 gctcatgata tgtgaataaa acaatacaat ttttaataga atattattgc atgcatgttt
     1141 tttctggcta caataaacac aagtatgcaa tattgtaaac acttttttat aaaaaggaat
     1201 tataatgccg atgaatacta caggtacgtc tttcagctct tttggtataa gttgccatag
     1261 agaaaactcc ttcaggaact ctttccgagg gaagaatgat gaggtcataa aatgttcaat
     1321 gggagaacgg acgattcgct tttctgttcg caaatttagc ggcaatatac tggatacagt
     1381 gagtaggcag agtactaaag atattaatgg gtggataaaa gatgaacgga tagtatatcc
     1441 ctcaagggtg atcaaccagg agattgataa ttactgtttt caaaaaaatg caaaaatttc
     1501 cactgaagag aggcaaaggg tcttttctct tgtgagtcag gaatatcaac taactcttga
     1561 tgttaaggca gcacaaagtt ctattaatca tgtgataatg gggaatgctt cttttggcaa
     1621 gaaaatagat gctctttgtg atagtatgag ccgggatgta aaaaatcgta cggcagatag
     1681 catagcaaac ttgcttgcag acaagtttta ccagaaacat atcgagccgg atattgatat
     1741 tgtaaaacta cgaaatgaga tcccagatta tttgaggtgt gctatacagg cataagtctc
     1801 tgggagacga ctaggtcagt atcattggtt ttgctctggt aaaaagagac gcggaaaaat
     1861 gcacaacagg cgccatacgt catgcatgga ttaggattgt tcatttttat accatttgtt
     1921 gttttttata gctttttgct aaaagggtga agaaaaaacg ttctccaaaa ttagattgca
     1981 actgtttgat tttatggata gaggcatgca gtttactcga cagtaaaaag catgccaaat
     2041 ttgctattaa ccttttgaat ataaaacact aatcgtctat ttctgttgcc ttcttattca
     2101 gcagacgaga agcgcgccag taaaccagca acatcaggcc cattacgata gtcagcgtaa
     2161 tgctggtggc tgcaccaaat ggccagtcac gaatattgag gaactgtacc ttgatgacgt
     2221 taccgatcag caggtttttc gcaccgccca tcaggtcgga tacatagaac aggcccatcg
     2281 ctggcagcat caccagcaga catccggcaa taattcccgg catcgtcagt ggaataatga
     2341 tacggataaa agtctgtaac ttgctggcac cgagatcgcg cgccgcctca agcaacggct
     2401 tatccagttt ttcgatactg gagtacagcg gcatcaccat aaacggcagc agaatgtaaa
     2461 ccagaccgat aatcaccgca ctgggcgtga acatgatacg gattggtgtg tcgataacgc
     2521 ccagccagag caaaaactcg ttgagatagc ctttggtgct gaggaaaatt ttcagcccgt
     2581 agatacgaat caatgagttg gtccagaagg gaacaatcag cagaaacagc agcagcggac
     2641 gcaccttgtg tggcagcttc gccagaaacc aggcaaacgg gtagcccagc accaggcagg
     2701 cgagggtggc gatcagcgcc atattcagcg agtgcaatag cacttcaaaa tagagcggat
     2761 cgagcagacg cgtgtagtta tccagcgtaa agaccatttt tacgaaactg gcgtcgtcgc
     2821 gggtcaaaaa gctggtgcca atgatcatca ggttgggcag aaagacaaac aacacaagcc
     2881 aaccgacaat agtgacaatc actacattct ggaacttact tgtgttcttc atcagccagt
     2941 acgacctccc agctttctac ccaattaatg gccatttttt ggtcgagaga gtggtcaaag
     3001 tcaggatcgt cttcattgaa gaattcgctg accatcacca tcttgccatt ttccagttca
     3061 acaaccgact ccagcgtcat gcctttgtag ttacgctcac gaacgtaacc aatcagccct
     3121 tcagcgtggt tgtcgtcgtt aatctcttca acacgtaagt cttccgggcg cagcagaaca
     3181 tgcagttttt gccccggttc aacggcgaag ttaacgtaga tattacattc gcggccttca
     3241 acgttggcgc gtacgcgctg ctcgtctaga cgttcgatga cagtggcgtt aaacatattg
     3301 atttcgccaa tgaagccggc aacaaacagg tttttcggct cttcgtaaat ttcgcgcggt
     3361 gtaccatctt gctcaatacg accctcgcgc atcaccacaa tcctgtctga catggtgagc
     3421 gcttcttcct gatcgtgagt gacaaagacg aaggtaatac caagcttacg ctgtaacgct
     3481 ttcagctcgt tctgcatttg cttacgcagt ttgtaatcca gcgctgagag cgactcatcc
     3541 agcaacaaca gacgaggctt gttaaccacc gcgcgagcaa tggcgacgcg ttgctgttga
     3601 ccaccagaga gctgatgcgg tttacgttga gcgaaggttt ccagttgcac catccgcagg
     3661 gcttccatca cgcggggcgt aatttcagca gcgggggttt tttgcatgcg caacccaaag
     3721 gccacatttt cgaacacggt catgtggggg aaaagtgcgt agctttggaa aacagtgttc
     3781 acatagcggt tttccgccgg aacgtgggtg atgtcctcgt tatccagcat gatgcgtccg
     3841 gaatcaacag tttccagacc tgcaatcagg cgaagaacgg ttgttttacc gcagccagaa
     3901 gggccaagca gcgtgaggaa ctcgccattg ttgatagtca gatccagctg gggaatgacc
     3961 tctttaccat caaagcattt gcgaattccc gccaattgca ccagcggtga aagcgaactc
     4021 ggttgtttat tcaatttttt actctgtccc atgtaaacgc aacggatggc ttaccgatgc
     4081 ggggtttgtg gttaaccacc ttggtgactc ttaatgaggg cggtaattct acggcaaacc
     4141 gcttgaatcg ccaatctttg ttgtgaatta ctggcttagc tttatattca ttaaggtaat
     4201 gctgataaat attcccgctt gcaggggtaa aagtgacctg acgcaatatt tgtcttttct
     4261 tgcttcttaa taatgttgtc acaaaaagtg agggtgacta catggataaa ctacttgagc
     4321 gatttttgaa ctacgtgtct ctggataccc aatcaaaagc aggggtgaga caggttccca
     4381 gcacggaagg ccaatggaag ttattgcatc tgctgaaaga gcagctcgaa gagatggggc
     4441 ttatcaatgt gaccttaagt gagaagggca ctttgatggc gacgttaccg gctaacgtcc
     4501 ctggcgatat cccggcgatt ggctttattt ctcatgtgga tacctcaccg gattgcagcg
     4561 gcaaaaatgt gaatccgcaa attgttgaaa actatcgcgg tggcgatatt gcgctgggta
     4621 tcggcgatga agttttatca ccggttatgt tcccggtgct gcatcagcta ctgggtcaga
     4681 cgttgattac caccgatggt aaaaccttgt taggtgccga tgacaaagca ggtattgcag
     4741 aaatcatgac cgcgctggcg gtattgcaac agaaaaacat tccgcatggt gatattcgcg
     4801 tcgcctttac cccggatgaa gaagtgggca aaggggcgaa acattttgat gttgatgcct
     4861 tcgatgcccg ctgggcttac actgttgacg gtggtggcgt aggcgaactg gagtttgaaa
     4921 acttcaacgc cgcatcggtc aatatcaaaa ttgtcggtaa caatgttcat ccgggcacgg
     4981 cgaaaggagt gatggtaaat gcgctgtcgc tggcggcacg tattcatgcg gaagttccgg
     5041 cggatgaaag cccggaaatg acagaaggct atgaaggttt ctatcacctg gcgagcatga
     5101 aaggcaccgt tgaccgagcc gatatgcact acatcatccg tgatttcgac cgtaaacagt
     5161 ttgaagcgcg taaacgtaaa atgatggaga tcgccaaaaa agtgggcaaa gggttacatc
     5221 ctgattgcta cattgaactg gtgattgaag acagttacta caatatgcgc gagaaagtgg
     5281 ttgagcatcc gcatattatc gatatcgccc agcaggcgat gcgtgactgc gatattgaac
     5341 cggaactgaa accgatccgc ggcggtaccg acggcgcgca gttgtcgttt atgggattac
     5401 cgtgcccgaa cctgttcact ggcggttaca actatcatgg taagcatgag tttgtgactc
     5461 tggaaggtat ggaaaaagcg gtgcaggtga ttgtccgtat tgccgagtta acggcgcaac
     5521 ggaagtcgtg attgcccgat gcgtatgtgg actgccaatt accgggatga aaccgttata
     5581 ttgatactgg tccacatact tatcgtgcct acaggcgttc agtgcatgta ggcacgataa
     5641 ggcgcaatca gcgccgcatc cggcttaaat ccaaacttac ccttcgaaga accaataccc
     5701 gctattgacc agcgccgcga gcatcgcgag gaatgacgga tcttccagcg catcgccaaa
     5761 attctccgca gtcagcgcaa tgttgctggc gagtgcatcc agtgccggac ggtgcgggga
     5821 atcgatcttc tcaccattgg catacacgtc gtcgccaatg cgcaatacgc gcagaccacc
     5881 caggcgcacc agcacttcac cttgtttcag cgcatcgtag atttcatctg gctgataagg
     5941 cggttccggc ggcgcgatat ccagttcatg acgtgactgg gatataaact cgccaaacca
     6001 ttgcttaaag tgttccggct ggttgatcaa ttcgagcatc atctcacgca gtttatccat
     6061 ctcttgcggc agaacgtccg caggatgagc gcgaggtgga acatccggat cgctgtagta
     6121 gttgccgccc agttcacgtt gcagcacata atcggcaaat ccgctaatta attcccgcgt
     6181 atttggcgcg cgaaaaccca cggaatagtt catcgcattt tccagcgcgt agccttcatg
     6241 cgggaatcct ggcggaatat aaagaatatc gccaggctcc agctcttcat cgatgatggc
     6301 ttcgaacgga tcgacctgta acagatccgg gtgcggacag tgctgtttca tttgcagctt
     6361 ttcgcccact cgccagcgac gacgaccggt accctgaatg ataaacacgt cgtactgatc
     6421 gagatgcggg ccgacgccgc cgcccggtac agaaaaagag atcatcagat catcaatacg
     6481 ccagtccggt agttcacgga acggtcgcat cagcgcggcg gtcggctcat gccagtggtt
     6541 cactgcctgt accagtaatg accagttggt ttcaccgaga tgatcgtagc tttcgaacgg
     6601 gccgtggctg acctgccatt tgccatcctg gtgactgacc agtcgactgt caacttcgct
     6661 ttccatcgcc agacccgcca actcgtctgg agagatcggg tcaataaaat tattaaagcc
     6721 gcgttttaac accaccgggc gtttctgcca gtgacgttca agaaaatcgg gccagttaag
     6781 agtgagttgg tattccatgt taagcatcca caggctggta tctgcaaccg attataacgg
     6841 atgcttaacg taatgcgtga agtatggaca tattttatca attagtgttt tttgccgttt
     6901 cataatgttt cagattgttc attatgtttt tgattataaa cagatttgtg attttccttg
     6961 ttccagcttg tttcacgttg tagccctttg tgcaacgatc ggtgtagtta atggtgtagt
     7021 taattgtgat gaccggaatc aaaattatga gcagagcact taacaaactg agcgatacac
     7081 agctgaggaa aatcaacggc acacccgccc aaaaaacagc ctttcttaat gacggtggaa
     7141 acctgagcgt caggcattca accagtggcc ttttaacctg gtatttcact tacagggccg
     7201 gaacgggaag gggggcacca ccggaacgca ttaagctggg aaattatcct gatctgagcc
     7261 tgaaatcagc cagggaaaaa gccgcccagt gtcgcgcatg gctggcagag gggaaaaatc
     7321 cacgtcatga gcttaattac accgtacagg aagcgttaaa gccggtaacg gttggcgatg
     7381 cgctcaccta ctggcttgag tcgtacgcaa aggaaaaccg cgtggattat gccgccctga
     7441 aaaagcgcct taataatcac gtaatacagc acattggtgc tatgccgctg gataaatgcg
     7501 agctacggca ctggctggcc tgttttgacc aggtggcaaa gcgaacgcct gttactgccg
     7561 gattcttgct acagacgtgc aaacaggcgc ttaagttctg ccggaggcgg cgctatgcaa
     7621 tcagcaacgt tcttgatgat atgagtgtgg cggacgttgg gaaaaaaccg gatataagcg
     7681 agcgtgtctt aagcaccaaa gaactgggcg aattattgca ggcactggac aaaaaaatat
     7741 tctcccccta ctacatcgcg ttaatccgcc tcctgattgt gttcggatgc cggacggtag
     7801 aactgaggtt atcggagatc agcgagtggg attttaccga aatgctctgg accgtgccga
     7861 aagaacacag caaaacgaag gtggcaatat tccggcccat accggaagca atactgccgt
     7921 tcgtcacgca gctggtggag cagaacaggc acacgggctt attgctgggg gaagtgaaac
     7981 aggaaacaag cgtgtcgcag tacggcagat tagcgcacag gaggctaaat caccctcact
     8041 ggtcactaca tgacatccgg cgcaccttta caacgatgct gagcgattta ggcgtggatc
     8101 ctcacgtcgt ggagcagctt acaggccacc agatgccagg aatgcagcga gtttataatc
     8161 attcccgtta tctggatgct aaacgcaatg cgctggatat gtggatggag cggttaggga
     8221 tactggcggg aacacatgaa aacgtaaccg cactaccagc agccagaaga aaataatttt
     8281 tttcgtgttt tttcagtatg cgcatactgg ataagcacac agatacaacg gcgaacaatg
     8341 tgaaacaacg tggaacaacc acgaacaaga ggcgaaaaag tacacaggtt tataagtgac
     8401 tgattttatt tgtgtgactg tttttttata cattagcgaa taacgcttta acgcgtgaga
     8461 aaaaaggcat gaaacaatat tttatgcctt tgtattaaca tgaatttaaa agattttatt
     8521 cttactgttt cattttctgt aaatcataca tcctcaatat ttgccattca tcactctagc
     8581 gggaacaata cgacacaata agacacctga tgactcttta agaaacgaaa ggggggcaat
     8641 agtgttaagc actgatcggt ttatacgtga aaaagaatgc gaaaaactaa ccggccttag
     8701 ccgtacgtgt cgctaccgcc tggaaaaggc cggacaattc ccatcacgtc gtaaacttgg
     8761 cggtcgttcc gttggctggt ctttatccga ggttctggcc tggaaggata gctgcaaggc
     8821 agttcattaa tcacgctggc ggcacgcagc cgccacacat caatcatctg aacacagagc
     8881 tataaccatg aagattgaat atacgccaga acgtgggcgg ggattcgttc gccctggtga
     8941 aactgaaaaa caacaaaatt ggggtttttc agacataaaa aaagcggccc cgaaatggag
     9001 ccgcctttct gaacagataa cccgctgcgc cgtttgtgtg tgtgatccca aacataagaa
     9061 cggcaatgat agtcgctatc aggctggtgg gcaatgctat cagtctggtt cagttcgttg
     9121 ccatacctgc aatgagcgct tttccctgta ctctttaagg aattgctcaa gggcaaaagc
     9181 acatggcgcg aatctttctg attcatgctc tatctttctg cgccgtcttt tccgtgccgg
     9241 tgataatgtt ttggtcaatt ctttatcggt cattgtgttg tcctgcatag caatgcgccg
     9301 taatacctta caccacggcg ctgatggtga ttactctggt tctttggcct tgcgccgctg
     9361 gcgatattca acttctcgtt ttaatgctgc agtaacaaat tgccccgtac tttcgccagg
     9421 catttttacc gcctcaacat tgttcataac ttcatgcgga acccttgccg caacggtttg
     9481 tgattttgcg tttactgctt ttgtcgccat gtggtgtacc cctcataaaa aacaaatgca
     9541 gtatgcagga aaaaaaataa gtgttcaaca cttgacgtgt ttaacacctg ggtttaaatt
     9601 ggtgttcaac accttgttgg cgcaaggtgc agaaacgacg aaacccggca gtgcgctaac
     9661 actaaccggg cttctaacca ccaacgatag cgaaagtatc gaggtagcta tgagaaatca
     9721 taccacacac ccgcaagggc gggactcgca caacctgaat aaatacatct ggcgttttat
     9781 cgccctgagc acggcacaac cgcgcgtgat taccattgag gccaccagcg aacaggaagc
     9841 acgccagcaa tctccggctg gctgcgtgat ggtattcgct gcccgtattc gtcaggaggt
     9901 gtgccatgtg caataacatc cgtccggacg cagccgccga agccatcata acgctaatgc
     9961 acgcgctgat tgatatttct gttatcgcag acatggcgca taagcacgcc accagtgaaa
    10021 cagaatatgc cggggctttc gttcctcatt cgctggcggt tatgcaactt agtgcggata
    10081 tggcgctgaa tgaggccaaa gccatcctga ttgctgattg tgaaaatggg ggggttatgc
    10141 gtgatgatcg ttttaattcc ctgaaacagg aattttccgg cgttcctgat gatgcggctg
    10201 atgcgctttc gtcaatgcca gaacttatta gagcggcttt tttcttactt tccacgagag
    10261 aatataaatc aacggggctt gatgtactga atatcgccgc cgattatgcg gaatatgtgg
    10321 cagaggcgcg ttatagaaga aaatttcctg aggatgtaag ccatgcgtga tatttaccac
    10381 caaacaatag accgcgcatt tcttgcactt tctcacagtg aaaacatgct ggaaatattg
    10441 cgcatatggc ttgaaacact tggcgacaat gaacgcgaca aacaaaaatc aagaattgcc
    10501 acggcattaa taacgcttct tgatcctgta ataatggaac tgcaagaaat agatctattg
    10561 cacgacagat ataaagaaca gcacaccgga gaataaaaat aatgaaactt aaatattctg
    10621 gcttaactgc cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc
    10681 agtacggcgg cacggtaatg attaagggcg tggaagaacg gcgcgtaacc taccgccgtg
    10741 aaggttacga atatgattgc gtgatgcctg tttatcagtt ccggcgtgat ttttctctgg
    10801 tacagaccgc gccgcataac gtgcccacca gcaacgccag ggcacgggca aacatccaga
    10861 agctgaaaac catgattaac ggattcaggg gcaagaaatg aaaagcgcac cgaacttaaa
    10921 aaaacagcct tacgacaaga tgaccgaagt cattattttt gcgggtagtg atgcctgggc
    10981 acatgcgaaa cagtggcagg aacaggacgg gcgactggct ggcgataatg tgcctcccgt
    11041 tgtgctggct gatgatcaac tggatgaact ggcagacctg agaatcatcg acgaggggcg
    11101 ctattgtgtc cggctgtaca aggcaggcca catcaggcca tcaaatatta atgccattgc
    11161 gcacaagctg gcggcggcgg gtgtaactga tgcgaattat ta
//
LOCUS       DAZFJF010000133        11161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFJF010000133 DAZFJF010000000
VERSION     DAZFJF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.134"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..805
                     /locus_tag="ACHKAU_004065"
     CDS             <1..805
                     /locus_tag="ACHKAU_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO5533030.1"
                     /translation="TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAI
                     PELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEGKFPLK
                     ELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIA
                     EQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNA
                     AELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1074..3311
                     /gene="nrfB"
                     /locus_tag="ACHKAU_004066"
     CDS             1074..3311
                     /gene="nrfB"
                     /locus_tag="ACHKAU_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO5533031.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3298..6270
                     /gene="nfrA"
                     /locus_tag="ACHKAU_004067"
     CDS             3298..6270
                     /gene="nfrA"
                     /locus_tag="ACHKAU_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO5533032.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6271..7161
                     /locus_tag="ACHKAU_004068"
     CDS             6271..7161
                     /locus_tag="ACHKAU_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO5533033.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7344..8105
                     /gene="envY"
                     /locus_tag="ACHKAU_004069"
     CDS             7344..8105
                     /gene="envY"
                     /locus_tag="ACHKAU_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO5533034.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8618..9571
                     /gene="ompT"
                     /locus_tag="ACHKAU_004070"
     CDS             8618..9571
                     /gene="ompT"
                     /locus_tag="ACHKAU_004070"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO5533035.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9758..>11161
                     /locus_tag="ACHKAU_004071"
     CDS             9758..>11161
                     /locus_tag="ACHKAU_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5533036.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDK"
BASE COUNT         3032 a         2745 c         2707 g         2677 t
ORIGIN      
        1 aacgctccgg cactcttatg acaagagtcg ccgcagggga gcgattcatg tcattagtgc
       61 gttctcaaca atgcacagtc tggtcatcgg acagatcaag acggatgaga aatctaatga
      121 gattacagct atcccagaac ttcttaacat gctggatatt aaaggaaaaa tcatcacaac
      181 tgatgcgatg ggttgccaga aagatattgc agagaagata caaaaacagg gaggtgatta
      241 tttattcgcg gtaaaaggaa accaggggcg gctaaataaa gcctttgagg gaaaatttcc
      301 gctgaaagaa ttaaataatc cagagcatga cagttacgca atcagtgaaa agagtcacgg
      361 cagagaagaa atccgtcttc atattgtttg cgatgtccct gatgaactta ttgatttcac
      421 gtttgaatgg aaaggtctga agaaattatg cgtggcagtc tcctttcggt caataatagc
      481 agaacaaaag aaagagcccg aaatgacggt cagatattat atcagttctg ctgatttaac
      541 cgcagagaag ttcgccacag caatccgaaa ccactggcac gtggagaata agctgcactg
      601 gcgtctggac gtggtaatga atgaagacga ctgcaaaata agaagaggaa acgccgcaga
      661 attattttca gggatacggc acatcgctat taatatttta acgaatgata aggtattcaa
      721 ggcagggtta agacgtaaga tgcgaaaagc agccatggac agaaactatc tggcgtcagt
      781 ccttgcgggg agcgggcttt cgtaatcttg ccctgcctgc tcacctcctc caggcacttc
      841 cactacatta tgacctgctc ggtgccaccg ctccttttca ctatgacgtt aacctcccct
      901 ccttgacacc atgcttttgt ctacattcta cgcaaacggc aacttttata accagtgtaa
      961 aaataacgtg ccgcaatagt catacctatt aatggtaaaa agctgtcaca attcataaaa
     1021 aaccttaata tacgccaccc taaacataac cagcgttaat gtaaggtttt tgtgtggact
     1081 ggcttcttga tgtttttgca acctggctct acggcttaaa agtaatcgcg ataacgttag
     1141 cggtcatcat gttcatcagc gggctggacg atttttttat tgatgtcgtc tactgggtac
     1201 gccgcattaa acgcaagttg agtgtttatc gccgctaccc gcgaatgagt taccgcgaac
     1261 tgtataaacc agatgaaaaa ccgttagcga ttatggttcc ggcgtggaat gaaacgggcg
     1321 tcatcggcaa tatggccgag ctggcggcga ccacgctcga ctacgaaaac tatcatatct
     1381 ttgttggcac ctaccccaac gaccccgata ctcaacgtga tgttgacgaa gtgtgcgctc
     1441 gctttccgaa cgtgcataag gtagtctgcg cgcgtcctgg tcccaccagc aaagccgact
     1501 gtctgaacaa cgtgctggac gccatcaccc agtttgaacg tagcgccaat ttcgcttttg
     1561 ctggttttat tctgcatgac gccgaagatg tgatttcacc gatggaattg cgtctgttca
     1621 actatctggt cgagcgtaaa gatctgattc agatcccggt gtatccgttc gaacgcgaat
     1681 ggacgcactt caccagcatg acttacattg atgagttttc agagctgcat ggcaaagatg
     1741 ttccggtgcg tgaagccctc gccggacaag tgcccagcgc aggcgtcggc acctgtttca
     1801 gccgccgcgc cgtgaccgct ctgttagctg acggtgacgg tattgctttc gacgtgcaga
     1861 gtctgactga agattacgac attggtttcc gcctgaaaga gaaaggtatg acggaaattt
     1921 ttgtccgttt tccggtggtg gacgaagcca aagaacgcga gcagcgtaaa tttttacagc
     1981 acgcacggac gtcaaacatg atctgcgtgc gcgaatattt ccccgatacc ttttcgactg
     2041 cagttcgaca aaaatctcgc tggatcatcg gcattgtttt ccagggcttt aaaacccaca
     2101 aatggacctc cagcctgacg ctgaactact ttctctggcg cgaccgcaaa ggggcaatca
     2161 gtaactttgt cagcttcctc gcgatgctgg tgatgctcca gcttttgctg ttgctggcgt
     2221 atgaaagttt gtggcccgat gcctggcatt tcctttctat ttttagcggc agcgcatggt
     2281 taatgaccct gctgtggcta aactttggct tgatgtttaa ccgcatcgtg cagcgggtga
     2341 ttttcgtcac tggctactac ggtctgacgc aggggctgct atctgtcctg cgtcttttct
     2401 ggggcaacct gattaacttc atggccaact ggcgcgcgct aaaacaggta cttcaacacg
     2461 gcgatccacg tcgcgtcgcg tgggataaaa caacgcatga cttccccagc gtgactggcg
     2521 atacccgctc gttgcgcccg ttaggccaaa ttctgctgga aaatcaggtc atcactgaag
     2581 aacaactcga tacagcactg cgtaatcgcg tcgaaggtct acgcctgggc ggttcaatgc
     2641 tgatgcaggg gctgattagc gccgagcagc tggcacaggc gctggcagag caaaacggcg
     2701 tggcgtggga atccatcgat gcctggcaga tcccgtcctc gctgattgcc gaaatgccag
     2761 cctccgtagc gctgcattat gcagtactgc cgctgcgtct ggaaaatgat gagttaattg
     2821 tcggcagtga agatggtatt gaccctgttt cgctggcggc cctgacgcgt aaagtcggac
     2881 gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt cacggggtta cgtcactggt
     2941 atgcacgccg acgcggtcac gatccgcggg caatgttgta caatgcggtt cagcatcagt
     3001 ggctcacgga acagcagacc ggtgaaatct ggcggcaata tgtgccgcat cagttcctgt
     3061 tcgccgaaat actgaccacg ctcggtcata ttaatcgttc agcaattaac gtgttgttat
     3121 tgcgccatga acgcagttct ctgccgctcg gcaagttttt ggtcaccgaa ggcgttatca
     3181 gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga attacaagtt tcgatgcaat
     3241 cactattact caaagcaggt ttaaacacag aacaggttgc gcaactggag tccgaaaatg
     3301 aaggagaata accttaatcg cgtcatcgga tggtctggtt tactgctgac gtctttattg
     3361 agtaccagcg cactcgcaga caatatcggc accagcgcag aagagctggg gctgagcgat
     3421 tatcgccatt ttgttattta tccccgtctc gataaggcgc tgaaggcaca gaaaaataac
     3481 gacgaagcaa ccgccatccg cgaatttgaa tatatacacc agcaggtgcc ggataatatt
     3541 ccgctgactt tataccttgc ggaagcctat cgccattttg gtcatgatga ccgggcgcgg
     3601 ctgttgctgg aggatcaact gaaacgtcac ccaggagatg cccgacttga gcgcagtctg
     3661 gcggctattc cagttgaagt gaaaagcgtt acgactgttg aagaactgct tgcccagcaa
     3721 aaagcgtgcg atgctgcgcc gaccctgcgt tgtcgcagtg aagtcgggca gaatgccctg
     3781 cggctggcac agttacctgt cgccagagcg caactgaacg atgcgacgtt tgctgcatcg
     3841 ccggaaggaa aaacgctgcg aaccgatctg ctgcaacggg caatctacct gaaacaatgg
     3901 tcccaggcag atacgctata caatgaagca cgccagcaga acacattaag cgcggcagaa
     3961 cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg acgatcggat cctggcactg
     4021 caatcacagg ggatcttcac cgatcctcag tcatatatta cttacgcgac cgcgctggct
     4081 tatcgtggcg aaaaagcacg cctccagcat tatctcattg aaaataagcc gctgtttacc
     4141 acggacgcac aagagaaaag ttggctctat ctgttatcta aatacagcgc caaccccgtt
     4201 caggcattgg cgaattatac ggtgcagttt gccgataacc gccagtatgt tgttggcgcg
     4261 acgctaccgg tgctgttaaa agaaggtcag tacgacgcag cgcaaaaact gctcgccacc
     4321 ctccccgcca atgaaatgct tgaggagcgt tatgctgtca gcgtggcgac ccgtaacaag
     4381 gctgaatctc tgcgtctggc acgattgctg tatcagcaag aaccggcaaa tcttacccgc
     4441 ctagatcaac tgacctggca actgatgcag aacgagcagt cacgcgaagc tgccgattta
     4501 ttgctgcaac gctatccttt ccagggcgat gcgcgtgtca gccagacttt aatggcgcga
     4561 ctggcgtctc tgctggaaag tcatccttac ctggcaactc cagcgaaggt ggcgatttta
     4621 tcaaaaccct tgccgctggc ggcgcaacgt cagtggcaaa gccagttgcc gggtattgca
     4681 gataattgcc cggcaatagt tcgcttgctg ggcgatatgt cgccttccta cgatgccgcc
     4741 gcctggaacc gtctggcaaa gtgttatcgg gacacgctac ccggtgtggc cttgtatgca
     4801 tggcttcagg ccgaacaacg acaaccgaac gcctggcaac atcgtgcggt agcctatcag
     4861 gcgtatcagg ttgaggacta cgccaccgca ctggcggcct ggcagaaaat cagtcttcac
     4921 gacatgagca atgaggatct gcttgctgct gccaataccg cccaggcggc aggaaatggt
     4981 gcagctcgcg atcgctggct acaacaggca gaacaacgtg gactgggaaa caatgccctc
     5041 tactggtggc tgcatgcgca acgttacatt cctggtcagc cggaactcgc actgagcgat
     5101 ctcacgcgct caatcaatat tgcgccttct gccaacgctt acgttgcgcg ggcgacaatt
     5161 tatcgccaac gtcataatgt cccggcggcg gtgagtgatt tgcgcgccgc gctggaactg
     5221 gaaccgaata atagcaacat ccaggcagcg cttggttacg ccttgtggga tagcggtgat
     5281 atcgcacagt cgcgggaaat gctcgaacag gcgcataaag ggcttccgga cgatccggca
     5341 ctgatccgac aactggccta cgtgaaccag cgtctggatg acatgcctgc gacgcagcac
     5401 tacgcccggc tggtgattga tgacattgat aatcaggcgc tgataacccc actgacccca
     5461 gagcaaaatc agcaacgctt caatttccgc cgtttgcatg aggaggtcgg tcgccgctgg
     5521 acgttcagtt tcgattcttc tatcggcttg cgttccgggg caatgagcac cgctaacaat
     5581 aatgtcggcg gcgcagcgcc agggaaaagc tatcgtagct acgggcaact ggaagccgag
     5641 taccgcatcg gacgcaatat gctgctggaa ggcgacctgc tctcagttta tagccgcgtc
     5701 tttgccgata ccggagaaaa cggggtgatg atgccggtga aaaatccgat gtccggcacc
     5761 ggtctgcgct ggaagccgct gcgcgatcag atctttttcc tcgccgtcga acagcagttg
     5821 ccgctgaacg gccaaaatgg cgcatccgat accatgctgc gcgccagcgc ctcattcttt
     5881 aatggcggca aatacagcga cgaatggcac ccgaacggtt caggctggtt tgcccaaaac
     5941 ctgtacctcg atgcggcgca atatatccgc caggatattc aggcgtggac ggcagattat
     6001 cgcgtcagct ggcatcagaa ggtagctaac ggacagacta ttgagcctta cgctcacgtt
     6061 caggacaacg gctatcgtga taaaggcact cagggcgcgc agcttggcgg agtcggggtc
     6121 cgctggaata tctggaccgg cgagacgcac tacgacgcct ggccgcacaa agtcagtctc
     6181 ggcgtcgagt atcaacatac ctttaaggcg attaatcaac gtaacggaga gcgcaacaac
     6241 gcgtttctca ccattggagt gcactggtaa atgcgtaagt tcattttcgt attgctgaca
     6301 ctgcttttgg tcagcccttt ttcctttgcg atgaaaggta ttatctggca accacaaaac
     6361 cgagatagtc aggttaccga tacccagtgg caggggctga tgagtcagtt acgtttgcaa
     6421 ggcttcgata ccctggtttt gcaatggacc cgttacggcg atgcattcac ccagccagaa
     6481 cagcgcgcgt tattgtttaa gcgtgccgca gctgcgcaac aggctggcct gaagctgatt
     6541 gtcgggctga acgccgatcc ggaatttttt atgcaccaga aacagtcgtc cgcagcgctg
     6601 gaaagctatc ttaatcgcct gctggctgcc gatctccagc aagccagatt atggagcgcc
     6661 gcgcctggcg taacgccgga tggctggtac atcagcgcgg aaattgacga cctgaactgg
     6721 cgcagcgaag ctgcccgtca gcctttgcta acatggttaa acaacgcgca gcggctgatt
     6781 agcgatgttt cagcaaaacc ggtttatatc agtagttttt tcgccggaaa catgtcgccc
     6841 gatggctatc gccaactgct ggaacaagtt aaagcaaccg gcgttaatgt ctgggtacag
     6901 gatggcagcg gcgtggataa acttaccgct gaacagcgtg aacgttatct acaggccagc
     6961 gccgattgcc aaagtcccgc ccctgccagc ggcattgttt atgaactgtt tgtcgccggc
     7021 aaaggcaaaa cctttacagc gaaaccgaaa ccggatgcag agatcgcctc gctgttagcg
     7081 aaacgttcct cttgcggtaa agacactctc tatttctctc tgcgctattt gcccgtcgcg
     7141 cacggcattc tcgagtatta aatctcctcc aggtaagtcg ggtacgacct ggcttacctc
     7201 tttcgctctc gtcagaattc catcaagatt aattgctaaa aagcgtgcta aagaaagaag
     7261 atcgttatcg gcgaacgggc cgatcactca tgcaggaagc atagatcctt tgagcggagt
     7321 ttctgatatc tgaggagtgc gaaatgcaat tgagcagcag tgaaccttgc gtggtgatcc
     7381 tgaccgaaaa agaggtagag gtaagcgtca ataaccatgc tacgtttacc cttccgaaaa
     7441 actacctggc cgccttcgcg tgcaacaata acgtcattga actctcaacg ttaaatcacg
     7501 tattaatccc ccacatcaac cgtaacatca tcaacgatta tctgttgttt ttaaataaga
     7561 acttaacctg tgtaaagccc tggtcgcggc tggcaacccc ggttatcgct tgtcatagcc
     7621 gtacaccgga agtgttccgg ctagccgcca accacagcaa gcagcaaccc agcaaaccct
     7681 gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt gtctaacttt cttgagcaat
     7741 cgcggtttat agccctactg atgtatatct tacgcagcag cgtccgcgac agcgtctgcc
     7801 gcattattca aagcgatatt cagcattact ggaatctgcg aattgtcgcc agttcgctat
     7861 gtttaagccc cagcctactc aaaaagaaat taaaaaacga aaataccagc tatagccaga
     7921 ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt attgatggat aacaaaaata
     7981 tcactcaggt ggcgcaatta tgtggctata gcagcacgtc gtactttatc tctgttttta
     8041 aggcgtttta cggcctgaca ccgttgaatt atctcgccaa acagcgacaa aaagtgatgt
     8101 ggtgaagggc aaagcggaaa cggataagac gggcataaat gaagaagaaa tggcgcgccc
     8161 tgcaggattc gaacctgcgg cccacgactt agaagttcct agaacgacat tttaagtcaa
     8221 caaattaccg cgccatctct gcgctcacac gtcccactac ctcaaaacat gtaaagcctt
     8281 gcaagccatt gtgaggcctt atgtgtctca gttttgtccc tcttttttgt actaaaaaac
     8341 atagcaattg aggataaacc tcatgctatt ttcgcttata tgcctttaaa ggcatggcgc
     8401 ttaaatagat aaaaacacca caaaagcata aaaaaccaca cagtaaaacc gcaacataaa
     8461 acaataacag ataattaaac caaaaacaga tagcgcattg tgataatcat tcaatactaa
     8521 acaaaatata aacagtggag caatatgtga ttgactcatt aagttagata taaaaaatac
     8581 atattcaatc attaaaacga ttgaatggag aacttttatg cgggcgaaac ttctgggaat
     8641 agttctgaca acccctattg cgatcagctc ttttgcttct accgagacta tatcgtttac
     8701 tcctgacaac ataaatgcgg acattagtct tggaactctg agcggaaaaa caaaagagcg
     8761 cgtttatcta gctgaagaag gaggccgaaa agtcagtcaa ctcgactgga aattcaataa
     8821 cgctgcaatt attaaaggtg caattaattg ggatttgatg ccccagatat ctattggggc
     8881 tgcgggctgg actactctcg gcagccgagg tggcaatatg gtcgatcagg actggatgga
     8941 ttccagtaac cccggaacct ggacagatga aagtaggcac cctgatacac aactcaatta
     9001 tgccaacgaa tttgatctga atatcaaagg ctggctcctc aacgaaccca attaccgcct
     9061 gggactcatg gccggatatc aggaaagccg ttatagcttt acagccagag gtggttccta
     9121 tatctacagt tctgaggagg gattcagaga tgatatcggc tccttcccga atggagaaag
     9181 agcaatcggc tacaaacaac gttttaaaat gccctacatt ggcttgactg gaagttatcg
     9241 ttatgaagat tttgagctag gtggcacatt taaatacagc ggctgggtgg aagcatctga
     9301 taacgatgag cactatgacc caggaaaaag aatcacttat cgcagtaaag tcaaagacca
     9361 aaattactat tctgttgcag tcaatgcagg ttattacgta acacctaacg caaaagttta
     9421 tgttgaaggc gcatggaatc gggttacgaa taaaaaaggt aatacttcac tttatgatca
     9481 caatgataac acttcagact acagcaaaaa tggtgcaggc atagaaaact ataacttcat
     9541 cactactgct ggtcttaagt acacctttta acgaagttaa ccagattttc tccccggtgg
     9601 tattgtataa ccccggggaa tttgtttaca gttacaagta ggcaatatcc actaattttg
     9661 taagcattta acctatgtca taacgtgtaa tctcttaccg caacacaaga cctcaatata
     9721 ttcaaatctt atctttattc cgatattcag agacacaatg cgaaaaataa ttactcattt
     9781 caaagttgtt ttaacgttac ttctaccagt aaccgtatct gcccagcaga tacagtggca
     9841 atcctgtatg gccagtcaat tcaaccactg gtttggtgag gaaaaaccgt ctcctgactt
     9901 actatgtggt tatttgtctg ttccattaaa atatacagac acaggcggag atgcttctta
     9961 tgaaaaaaaa tcacaagtca aactagcgtt gacaaaattg ccggcaaaaa gcaagcataa
    10021 aggaagtatc ctgataataa gtggtggtcc cgggttacca ggcataaatc cttatattaa
    10081 ctttgactgg ccagtcacaa atcttcgtga gtcatgggat attattggat ttgatcctcg
    10141 aggcgtcgga cagtccactc cgacaataaa ctgccggcaa tcagatacag agactcagga
    10201 aaacataacc gaaaagcaac aagtattaaa taaaattaat gcctgtatcc ataataccgg
    10261 agccgaagtc attcgccata tcggctctaa cgaggctgta tacgatattg atcgtattag
    10321 gcaagccttg ggggataaac aactgacagc cgtggcgtat tcgtatggaa ctcaaattgc
    10381 agccttatat gcagaacgtt ttccctacaa cgtaagatct atcgttcttg atggagtcgt
    10441 cgatatcgat gacctggagg acaacttcac atggcaactc aaacaggcac agagttatca
    10501 ggaaacgttt gatcgctttg catcctggtg tgcgcgtaca aaaagttgcc cgctttcttc
    10561 agacagagat aaggcaataa ctcagttcca tgagctatta tcaaaattac atcacaaacc
    10621 tttattagac agtaagagag aaaatatatc ttcagatgaa ctcatatcat taacaacaga
    10681 ccttctgcta tggcgttcat catggccaac ccttgcaact gccatacgcc agttctctca
    10741 ggggattgtc agtaatgaaa ttgaaactgc gctcagtgct ccgatagcct cagaagagtc
    10801 aagcgatgct tcgggggtaa tcctctgtgt agatcagggg gatgagcaat taacaccaga
    10861 agagcgaaaa tcccgaaaag acgctcttgc gaatgccttc ccggctatta actttgacaa
    10921 tggacgttcc gattcacctg atttttgtga attatggcca atacatagcg acctgaacaa
    10981 aactcgcctg aaaaatactg ttctgccctc tggtttactg tttatagcac acaaatacga
    11041 cccaacaacg ccctggatta atgcccgtaa gatggcagag aaattttcca gcccgttact
    11101 aacaataaat ggtgatgggc atacattagc tctcaccgga gttaatttat gtgtagataa
    11161 a
//
LOCUS       DAZFJF010000134        11098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFJF010000134 DAZFJF010000000
VERSION     DAZFJF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.135"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..1102
                     /gene="ybhH"
                     /locus_tag="ACHKAU_004072"
     CDS             50..1102
                     /gene="ybhH"
                     /locus_tag="ACHKAU_004072"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO5533037.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1178..2611
                     /gene="ybhI"
                     /locus_tag="ACHKAU_004073"
     CDS             1178..2611
                     /gene="ybhI"
                     /locus_tag="ACHKAU_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO5533038.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKFLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2794..5055
                     /gene="ybhJ"
                     /locus_tag="ACHKAU_004074"
     CDS             2794..5055
                     /gene="ybhJ"
                     /locus_tag="ACHKAU_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO5533039.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5289..6572)
                     /gene="ybhC"
                     /locus_tag="ACHKAU_004075"
     CDS             complement(5289..6572)
                     /gene="ybhC"
                     /locus_tag="ACHKAU_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO5533040.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6707..7777)
                     /locus_tag="ACHKAU_004076"
     CDS             complement(6707..7777)
                     /locus_tag="ACHKAU_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5533041.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTH
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(7755..7973)
                     /locus_tag="ACHKAU_004077"
     CDS             complement(7755..7973)
                     /locus_tag="ACHKAU_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5533042.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(8080..8424)
                     /locus_tag="ACHKAU_004078"
     CDS             complement(8080..8424)
                     /locus_tag="ACHKAU_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533043.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(8453..8620)
                     /locus_tag="ACHKAU_004079"
     CDS             complement(8453..8620)
                     /locus_tag="ACHKAU_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533044.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(8693..8977)
                     /locus_tag="ACHKAU_004080"
     CDS             complement(8693..8977)
                     /locus_tag="ACHKAU_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO5533045.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(8970..9113)
                     /locus_tag="ACHKAU_004081"
     CDS             complement(8970..9113)
                     /locus_tag="ACHKAU_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533046.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            complement(9123..9272)
                     /locus_tag="ACHKAU_004082"
                     /pseudo
     CDS             complement(9123..9272)
                     /locus_tag="ACHKAU_004082"
                     /inference="COORDINATES: protein motif:HMM:TIGR03655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
     gene            complement(9749..9886)
                     /locus_tag="ACHKAU_004083"
                     /pseudo
     CDS             complement(9749..9886)
                     /locus_tag="ACHKAU_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9888..10445)
                     /locus_tag="ACHKAU_004084"
     CDS             complement(9888..10445)
                     /locus_tag="ACHKAU_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5533047.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            complement(10442..10999)
                     /locus_tag="ACHKAU_004085"
     CDS             complement(10442..10999)
                     /locus_tag="ACHKAU_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533048.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            complement(10996..>11098)
                     /locus_tag="ACHKAU_004086"
     CDS             complement(10996..>11098)
                     /locus_tag="ACHKAU_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO5533049.1"
                     /translation="KPMPSREELLKRKSFGSVNDNKYLNAMWRSGKK"
BASE COUNT         2637 a         2710 c         2683 g         3068 t
ORIGIN      
        1 aatgtaacca tatcgcaata agttatgttt ttaaattgag ggcattatta tgaaaaaaat
       61 accctgcgtg atgatgcgag gtggaacctc gaggggcgcg ttcctgttag cggaacattt
      121 acccgaagat caaacgcagc gcgataaaat attgatggca attatgggtt ccggtaacga
      181 tctggaaatt gacggtattg gcggcggtaa tccactgacc agtaaagtcg ccattattag
      241 ccgttccagc gatccgcgtg ctgatgttga ttatctgttt gctcaagtta tcgtccatga
      301 gcaacgtgtc gataccacgc ctaactgcgg caatatgctt tctggcgttg gggcattcgc
      361 cattgaaaat ggtttgattg cagcgacatc accggtcacc cgtgtgcgta tccgcaacgt
      421 caatacgggt acgttcatcg aagctgatgt gcaaacgcca aatggtgttg tcgagtacga
      481 gggtagtgcc agaattgacg gcgtaccggg tactgccgca ccggttgcgc tcactttcct
      541 gaatgccgct ggaaccaaaa ccggaaaagt tttcccgact gataatcaga ttgattattt
      601 tgacgatgtc ccggtgacct gtatcgatat ggcgatgcca gtcgtcatta ttccggctga
      661 atatctggga aaaacaggtt atgaattacc ggcggaactg gatgccgaca aagcattatt
      721 agcccgcatt gaatctatcc gtctacaagc gggtaaagca atgggcttag gtgatgtcag
      781 taatatggtt atccctaaac ctgtgcttat ttctccagcg cagaaaggcg gggcaattaa
      841 tgtgcgttat tttatgccgc attcttgcca tcgcgcgctg gcgataaccg gtgctattgc
      901 tatttccagt agttgtgcat tggaaggcac cgtcacccga caaatcgtcc cttctgtagg
      961 atacggcaat atcaatattg aacaccccag tggtgcgctc gacgttcatt taagtaatga
     1021 aggtcaggat gccacgacgt tacgcgcatc tgttattcgg acgaccagaa aaatattttc
     1081 cggtgaagtt tatcttccct gaaaaaattc gttgtcaaga taaggataat caataaagga
     1141 cttctgtatg agtcatacag aaagaacagg attttaaatg aataagaaat cgttatggaa
     1201 gctaattctg atattagcga tcccatgtat tattggtttt atgccagctc cggcaggatt
     1261 aagcgaactg gcgtgggtgc tttttggtat ttacctggcg gccattgtgg ggctggttat
     1321 caagcctttc ccggaacctg tcgtactgtt aattgccgtt gctgcctcca tggtggtggt
     1381 cggtaactta tccgacggtg cgtttaagac caccgccgta ttaagcggtt actcttcagg
     1441 tactacctgg ctggtgttct cggcgtttac cttaagcgcc gcatttgtga ccaccggttt
     1501 aggtaaacgt attgcctatc tgctgattgg taaaatcggt aacaccacgc tgggtctggg
     1561 ttacgttacg gtattcctcg atctggtact ggctccggca acaccgtcta acaccgcgcg
     1621 tgcgggcggt attgtgttac cgatcatcaa cagcgtggcg gtggctttgg ggtccgaacc
     1681 ggaaaaaagt ccgcgtcgtg tcggacatta cctgatgatg tccatttaca tggtcaccaa
     1741 aaccaccagc tatatgttct ttaccgcaat ggcggggaac attctggcgc tgaaaatgat
     1801 caacgacatt ctgcacctgc aaattagctg gggtggatgg gcactggccg ccggattgcc
     1861 gggcatcatt atgctgctgg tcaccccact ggtgatttat accatgtatc cgccagaaat
     1921 taagaaggtg gataataaaa ccatcgctaa agcgggcctt gccgaactgg gaccgatgaa
     1981 aatccgcgaa aaaatgctgc tcggtgtctt tgtgctggcg ctactgggct ggattttcag
     2041 taagtttctg ggggttgatg aatccactgt ggcaatcgtt gttatggcga ccatgctgct
     2101 gctgggtatc gttacctggg aagacgtggt taaaaataaa ggcggctgga ataccttaat
     2161 ctggtacggc ggtattatcg gattaagctc cttattatcg aaagttaaat tcttcgaatg
     2221 gttagctgaa gtctttaaaa ataacctggc atttgatggt cacggtaacg ttgctttctt
     2281 cgttattatt ttcctcagca ttatcgtgcg ttatttcttc gcttccggta gtgcctatat
     2341 cgttgctatg ttaccggtat ttgccatgct ggcgaacgtc tccggcgcac cgttaatgtt
     2401 aaccgcgctg gcactgttgt tctccaactc ctatggcggc atggttactc actatggcgg
     2461 cgcggcaggt ccggtcatct ttggcgtggg ttataacgat attaaatcct ggtggttggt
     2521 cggtgcggta ctgacgatat taaccttcct ggtgcatatc accctcggcg tgtggtggtg
     2581 gaatatgctg atcggctgga acatgctgta aatattctcg tcatacttca agttgcatgt
     2641 gctgcgtctg cgttcgctca ccccagtcac ttacttatgt aagctcctgg ggattcactc
     2701 gcttgtcgcc ttcctgcaac tcgaattatt tagagtatat ccatttatta tctttctgcg
     2761 cacttcacgg tgcgcagata tctggagcat ttgatgatca agttatctga aaaaggcgtg
     2821 tttctcgcca gtaataacga aataattgcc gaagaacatt tcaccggcga aattaaaaaa
     2881 gaagaagcca aaaaaggcac tattgcctgg tctattctct cttcgcataa tacgtccgga
     2941 aatatggata aacttaaaat taagtttgat tcattagcct ctcacgatat tacctttgtt
     3001 ggtattgtac agaccgctaa agcgtccggt atggaacgtt tcccgctgcc gtatgtgctg
     3061 accaactgcc ataactcact ctgcgccgtc ggcggcacta ttaacggtga tgaccatgtt
     3121 tttggtttat cggcggccca gcgttatggc ggtatttttg tgcctccgca tattgcggtc
     3181 atccatcaat atatgcgtga gatgatggca ggcggcggca aaatgatcct cgggtcagac
     3241 agccacaccc gttacggtgc attagggaca atggcagtcg gtgagggcgg cggtgagttg
     3301 gtaaaacagc tgcttaatga cacctgggat atcgactatc cgggcgtggt tgcggtgcat
     3361 ctgaccggaa aacctgcgcc gtatgtgggg ccacaggatg tggcgctggc tatcattggc
     3421 gcggtgttca aaaacggtta cgtcaaaaac aaagtcatgg agttcgttgg accgggcgtt
     3481 agcgcgctct ctaccgattt ccgtaacagc gttgacgtga tgaccacgga aaccacctgt
     3541 ttaagctccg tctggcaaac tgatgaagaa gtgcataact ggctggcgct gcacggtcgc
     3601 ggccaggatt actgccagct taaccctcaa ccgatggcgt actacgatgg ctgcatcagc
     3661 gttgatttaa gcgccatcaa accaatgatt gcgctgccgt tccacccgag caacgtgtat
     3721 gaaatcgaca cactgaacca gaacctgacc gacattctgc gtgagattga aattgagtcc
     3781 gaacgcgtgg cgcacggtaa agccaaactc tcgctgctgg ataaagtgga aaatggtcgc
     3841 ctgaaagtgc agcaggggat tatcgcgggc tgttctggcg gtaactacga aaacgtcatc
     3901 gcggcggcga atgcactgcg cggtcaatcc tgtggcaatg acaccttctc gctggcagtt
     3961 tacccgtcat cacagccggt gtttatggat ctcgccaaaa aaggtgtggt agcagatttg
     4021 attggcgcag gcgcaatcat cagaaccgcg ttctgcggcc catgctttgg cgcgggcgat
     4081 acgccaatca acaacggttt gagtattcgc cacaccacgc gtaacttccc gaaccgcgaa
     4141 ggctctaagc cagctaatgg gcagatgtca gcggtggcgt tgatggacgc tcgttctatc
     4201 gctgcgactg cggcaaacgg tggctattta acctctgcca gcgaacttga ttgctgggac
     4261 aacgtgccgg agtacgcctt cgatgtaacg ccgtataaaa accgtgttta tcagggcttt
     4321 gtgaaagggg caactcagca accgctgatt tacggaccga acattaaaga ctggccggaa
     4381 ttgggtgcgc tgactgacaa tatcgtcctg aaagtgtgct cgaagatcct cgacgaagtg
     4441 accaccaccg acgaactgat tccttccggt gaaacctctt cttatcgttc aaatccgatt
     4501 ggtctggcgg agtttaccct gtcacgccgc gatcccggtt atgttggcag aagtaaagcg
     4561 actgctgagc tggaaaatca gcgtctggcg gggaatgtca gcgagctgac agaggtgttt
     4621 gcgcgcatta agcagattgc tggtcaggag catattgatc cgctgcaaac tgaaattggc
     4681 agcatggtat atgcggtgaa accaggcgat ggttctgcgc gtgaacaggc ggcgagctgc
     4741 cagcgtgtga ttggcggtct ggcgaatatt gccgaagagt acgcgactaa acgctaccgt
     4801 tctaacgtca tcaactgggg gatgttaccg ctgcagatgg cggaagtgcc aacctttgaa
     4861 gtgggggatt acatttacat ccctggcatt aaagcggcgc tggataatcc gggtacgacg
     4921 tttaaaggtt atgtgatcca tgaagatgcg ccggtaacgg aaattacgct ctatatggaa
     4981 agtctgactg ctgaagagcg cgagattatc aaggcgggta gtttgattaa cttcaataaa
     5041 aaccgtcaga tgtaaaaagc gccatgtgaa tgtaggtcgc attcggcact tattgtcgga
     5101 tgcgatgctt gcgcatctta tccgacctac gaatcgcatc gaatctgtag gccagataag
     5161 gcattttcgc agcatccggc acttattgtc ggatgcgatg cttgcgcatc ttatccgacc
     5221 tacaaatcgc atcgaaccgt aggccggata aggcgtttac gccgcatccg gcaaatagtt
     5281 aattgctctt acttcttcgc ctctgcaacc actttactac ccacgccgcg gttattgtat
     5341 tcccacatgc ggttgtagtt agtgtcattc agattgcgct gtatttcgtc gttatcatct
     5401 acgctgccgg tattacccgc aaacggacga ttagagatca ccgcatcggc ccacggttta
     5461 gccgtgttaa aaccttcgtt gatggcgcta tcacggatca ccacctgacc gttggtattg
     5521 gcatcaacat ccagcgagcg gcccagttgc gccacaccat caccgaaagc attgaaacgg
     5581 ctgtttacgg cgaggaaacc gtagtaaatg ttggacagcg tagccggtgc aaacacatac
     5641 gcttcttgct gagtacgtga gttcaccacg cggaattcgg tgttatcgaa caccactgcg
     5701 ccgcgaccag aaacgatatc cacatcccct tcaatgtagc tgttggtcac cagcgtacgc
     5761 ggctgacgat tcgtttccag acggttctgc acaccgctgt tggtgacaaa gaaggtgttc
     5821 tgacgaccga gaatgttaac gttgttaatc tgtacctggt caccatcagt acgcagtgcc
     5881 accgccggat ggttacctgc atctacgcta tcgcccagcg tgttttcgat ggtcagattt
     5941 tgcagttgca ggccattgtt ttgtgaccag aagaccgcag agcagagaac accgatactg
     6001 tcgctgcgtt tgctctggca gctatcgtac atataccacg ctggtttacc tggcatatat
     6061 ttgccgcgcg ggttgacgtc gtgacgccag tcggcagggc tcatgccacc atcaagggaa
     6121 agcccaatct tcacatcaat cggtttttca cctgtaccgt acagagtaat tccacccgga
     6181 gcggcaggga catataccgt tccctgatac tcaccaggca tcacggcaat atactggcgc
     6241 ttgttggtac gcttgataat tgccgcatct accgccgcct gaatcgtggt atgcgttaca
     6301 ccttgagtgc ccgccgggcc gacaacaaag tcaggttgcg caggcagggt aatcggggaa
     6361 ggattccacg ctgctgcacc tggtgtcagg gatgcaaaat agtgttgagc atcgaaattc
     6421 tgcgcttctt ttgccgacag aatcgggcga gaagaggtac caggcgcggt ttgatcagaa
     6481 ggacgttgat cgggcggggt tgagctacag gcggtcagcg tcacgccaaa agccaatgcc
     6541 agcgccagac gggaaactga aaatgtgttc acaggttgct ccgggctatg aaatagaaaa
     6601 atgaatccgt tgaagcctgc ttttttatac taagttggca ttataaaaaa gcattgctta
     6661 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgatttcaa
     6721 ttttgtccca ctccctgcct ctgtcatcac gatactgtga tgccatggtg tccgacttat
     6781 gcccgagaag atgttgagca aacttatcgc ttatctgctt ctcatagagt cttgcagaca
     6841 aactgcgcaa ctcgtgaaag gtcggcggat ccccttcgaa ggaaagacct gatgcttttc
     6901 gtgcgcgcat aaaatacctt gatactgtgc cggatgaaag cggttcacga tgagtagatg
     6961 caattatggt ttctccgcca agaatctctt tgcatttatc aagtgtttcc ttcattgata
     7021 tcccgagagc atcaacatgc aatgttgtag ggatggcaat ttttacgcct gttttgcttt
     7081 gctcgacata aagatatcca tctacgatat cagaccactt catttcgcat aaatcaccaa
     7141 ctcgctgccc ggtaacaaca gccagctcca ttgcaagtct tagccaacat ggtgatgatt
     7201 ctgctgcttg ataaattttc aggtattcgt cagccgtaag tcttgatctc cttacctctg
     7261 attttgctgc gcgagtggca gcgaccgggt ttgttgttat atggccttca gctattgcct
     7321 ctcggaatgc atcgctcagt gttgatctga ttaacttggc tgacgccgcc ttgccctcgt
     7381 ctatgtatcc actgagcatt gccgcaattt cttttgtggt gatgtcttca agtggagcat
     7441 caggcagccc cctccttatt gctttaattt tgctcatgta atttatgaga gtcttctgct
     7501 tgattcctct gctggcgagg attttttcgt agcgatcaag ccatgaatgt aacgtaacag
     7561 aattatcact gttgattctc gctgtcagag gcttgtgttt gtgtcctgaa aataactcaa
     7621 tgttggcctg tattgcttca gtgattgcta tcctcctgtc tcggcctaat ccaaactctt
     7681 tacccgtcct tgggtccctg tagcagtaat atccattgtt tcttatataa agattagggg
     7741 gtaaatcccg gcgctcatga cttcgccttc ttcccatttc tgatcctctt caaaaggcta
     7801 cctgttactg gtcgatttaa gtcaaccttt accgctgatt cgtggaacag atactctctt
     7861 ccatccttaa ccggaggagg gaatatcctg cattcgcgca cccatcgacg aactgtttca
     7921 aggcttcttg ggcgtcgctg gcgtgcattc cactcctgaa gtgtcaagta catcgcaaag
     7981 tctccgcaat tacacgcaag aaaagccgca ttgatgcggc gatggtaggt ctggatatca
     8041 ttgagccatg aacaggcctc atcgagtgtg aggctgtggt tagtcctttc gtaactcgct
     8101 gattctcctg taagtctctg gtgctttgtt tccgtgtatc ttcatttcag acttcaacag
     8161 agcaacgagg gaatcccatt cgttgaggat gcctttgaat gccggaacgc gctttgcaac
     8221 cttgtcgaat gaatctctga tttctggaat cttctcaaca agtgcaacgc atcgtcggaa
     8281 gtctgctgcg tcatggggag cgccgaagtg atgaccatag atattctttt tcaggccaca
     8341 tgcgattgag gcaagagttg cgctactgat gccaacatcg ccagtcgatt gccatttcaa
     8401 aaccttcata gacaaatctg acatttcttg tctccaataa aaaaaacctc catcaggcgg
     8461 cttggtgttc tttcagttct tcaattcgaa tattggttac gtctgcatgt gctatctgcg
     8521 cccatatcat ccagtggtca tagcagtcat tgatgttctc tgcttcgata actctgttga
     8581 atggttctcc attccattca cctgtgactc ggaagtgcat ttatcatctc cataaaacaa
     8641 aatccgccgt agcgagttca gataaaagaa atccccgcga gtgcgaggat tgttattcat
     8701 tgccgatatt cacctttatc gcgaacacct ttaccggttt atcaccgaag tgcggatgtg
     8761 tgattgtctt gatttcatat ccgtcatacg gaacatcaat tctgcggctg gaatcgtcgc
     8821 gcttcggata tccctttgtg ataatcaggc ggtcatactc ccggaacata attcgcttat
     8881 tccaatagtc attacacagg cgatactctt ccgttttctc tcctcgaatc atggcatcga
     8941 agtattcacc tttgacggca agttgtaggt tagccacggt taacctcctg cggagtagcc
     9001 tttacaagca ctggtgcaaa tccatcttca ttaaggttat gaatatagac ttctgttctc
     9061 ctgcgctctt caacgtttaa tattgtttct ggatcataaa cccatacttt cattcgacta
     9121 ttccatgaag aaatcgcttc agatctagtt cgtttttctg gtccttgggc accacatttg
     9181 catgacacat agcgcatttt ccctctgata ctgaatgagt atccgatgtt aagcacagtg
     9241 gaaccacaga atgggcagcg atatagattc ataaatcatc caccttaggc gcagctgaga
     9301 cagcaacatt ccagaactca gaaaacatat tgtatgcacc agatagattg gaagtagcat
     9361 accctccaag ctcacttcta atctgaacgg ctctcatcat ttccggggtt aactccttcg
     9421 gcaccataac ccaaccatcc ggagttaccg gagagttgcc accgggaata ttttccggaa
     9481 tattttgttg tgcgttttgt ggttgttctg atttaccctg aagcatggcg gcgcggcagg
     9541 cgttccagcc agctgttcgc ccaagcgcgt aaacttcaga tggatcaaga taatcaatgt
     9601 catgcccgtc ctcatcgtcg ttctcaggta atgcagcagg tactaccggt actggcggag
     9661 cggcgtagac ctcaataatt ccattatcaa taggccattc tccatccttg aggtagtcac
     9721 ttgtgccgtc aacttgctgt tctgcaatgt ggaatgcacc tattggtttt gcttccagcg
     9781 atgccagagc aatccgtgcc agttccattt gttcgccact agtaagtccg ttatcaagcg
     9841 gggatttaat gaacaattcg atacgttctt tagtgatagt gctcatatca ctctcctttg
     9901 atgcgaatgc cagctgcgct tgctgattct tcatatgcgc gtttagcagc gttaaggatt
     9961 gctgccagtg gcgtatagcc gccatccatt cggattgtgt tgtggatgcc agccattgtg
    10021 tcgcgcaatt tgctgtggct agcagacagt tctgctatgc acttctctgc ggcatcggct
    10081 cgatcttttt cacgctggaa cattttgtga gtgcggtcta tagcttcgat tcgtgccgct
    10141 gatgtcttct gcacggcttc aagctcaaca cgcagcttcc caaccgtaag agcaatatcc
    10201 tcgttcttct ggtcgcggcg tttgatgtat tgctggtttc tttcccgttc atccagtagt
    10261 gccagcacgg tttctggccc ggtcagaaat ttgaaggcgt tgagcgcatc aatatccaca
    10321 ccgtaatcct taagttcctg ttcagttaac aaatcatcat caactggcaa cattaacagg
    10381 cgttccattg ccggaattgc acgctctgcc gcctcacgca gtgcctgata gtcaatcttg
    10441 ctcattggat gactccttta cgaagctgtt cggcgatatc ttcgagaacg ccatcagaga
    10501 atgaacggtc aaaatcgcct tccggcgcat tggccataaa ctcagtagag gtaagaatca
    10561 tccgggcaat atcagcggcg ttcttcgcag tatcatcaat aaaaccagct tcccaggcag
    10621 ccagcattct gttcgccaca aagtaagcgc cctccttgcg tgcttcagtc ttcacttcag
    10681 ccagaaaagc atcggtggct ggggtttcag taatatcatc ttcccattcg ctaaactcct
    10741 cacgacaaaa gtcattaaat tccttctcag attgcttaag cgaggaattt tcagcagcca
    10801 tcttcgcgca tttagcctca aggttatcaa tcgtgattcc agcagaacga cactcccgca
    10861 acgccgtttc tagttttgat tcaagttcac cgaacttacg gacaagatat tcatcgtttg
    10921 tttcgtcaac ctttaaatca cttgggatgc atttaccttt cagaaaacca tccatctcaa
    10981 ttagtgtcat ttgtttcatt tcttcccact ccgccacatc gcattcagat atttgttgtc
    11041 attaacagaa ccgaaactct ttctcttaag caattcctct ctcgatggca ttggcttt
//
LOCUS       DAZFJF010000135        11077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFJF010000135 DAZFJF010000000
VERSION     DAZFJF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.136"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /gene="speFL"
                     /locus_tag="ACHKAU_004087"
     CDS             <1..99
                     /gene="speFL"
                     /locus_tag="ACHKAU_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO5533050.1"
                     /translation="NNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            410..2608
                     /gene="speF"
                     /locus_tag="ACHKAU_004088"
     CDS             410..2608
                     /gene="speF"
                     /locus_tag="ACHKAU_004088"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO5533051.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2605..3924
                     /gene="potE"
                     /locus_tag="ACHKAU_004089"
     CDS             2605..3924
                     /gene="potE"
                     /locus_tag="ACHKAU_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO5533052.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3980..5620)
                     /gene="pgm"
                     /locus_tag="ACHKAU_004090"
     CDS             complement(3980..5620)
                     /gene="pgm"
                     /locus_tag="ACHKAU_004090"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO5533053.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5646..6191)
                     /gene="seqA"
                     /locus_tag="ACHKAU_004091"
     CDS             complement(5646..6191)
                     /gene="seqA"
                     /locus_tag="ACHKAU_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO5533054.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6376..7140
                     /gene="ybfF"
                     /locus_tag="ACHKAU_004092"
     CDS             6376..7140
                     /gene="ybfF"
                     /locus_tag="ACHKAU_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5533055.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7280..7573
                     /gene="ybfE"
                     /locus_tag="ACHKAU_004093"
     CDS             7280..7573
                     /gene="ybfE"
                     /locus_tag="ACHKAU_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO5533056.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7713..8243
                     /gene="fldA"
                     /locus_tag="ACHKAU_004094"
     CDS             7713..8243
                     /gene="fldA"
                     /locus_tag="ACHKAU_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO5533057.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8453..8539
                     /gene="uof"
                     /locus_tag="ACHKAU_004095"
     CDS             8453..8539
                     /gene="uof"
                     /locus_tag="ACHKAU_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO5533058.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8532..8978
                     /gene="fur"
                     /locus_tag="ACHKAU_004096"
     CDS             8532..8978
                     /gene="fur"
                     /locus_tag="ACHKAU_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO5533059.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9062..9388)
                     /gene="chiQ"
                     /locus_tag="ACHKAU_004097"
     CDS             complement(9062..9388)
                     /gene="chiQ"
                     /locus_tag="ACHKAU_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO5533060.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9438..10844)
                     /gene="chiP"
                     /locus_tag="ACHKAU_004098"
     CDS             complement(9438..10844)
                     /gene="chiP"
                     /locus_tag="ACHKAU_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO5533061.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPSEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2669 a         2742 c         2777 g         2889 t
ORIGIN      
        1 aataataacc gcttaatgcc tcatataagg cggacaaccc atatcatgaa gtttgcccat
       61 cgtaatagtt ttgactttca tttctttaat gcccgctagt cttctgacta aagggcaccc
      121 caacgtacag gtctccctga ctttaagcat tacaggttaa tacctgtatt cctcggtgct
      181 catatactgc taaccctttt aaactctaaa taattcgagt cgcagcactt gcaacttgag
      241 gtatgacgag tatagccagt taccgggctg gtctgggtta ttgcatctgc aaaaagcaaa
      301 ctactgattt atttatcagc ggtggagctt tgcttttttt ccggcgtgat cgatttctcc
      361 tttgagaaat tgaggacctg ctattacctg aaataaagag atgaacaaaa tgtcagaatt
      421 aaaaattgcc gttagtcgtt cttgcccgga ttgtttttcc actcatcgtg catgcgtgaa
      481 tatagacgaa agtaattata ttgacgttgc cgccattatt ttatcagtca gtgatgttga
      541 acgtggaaaa ctcgatgaaa tagacgctac tggctatgac attcctgttt ttattgcaac
      601 ggaaaatgaa gaacgtgtac ccgcagaata tctgccacgt atttctggtg tcttcgaaca
      661 ttgcgaatca cgtaaagagt tttatggacg tcagttagaa accgctgcca gccattatga
      721 aacgcaactg cgcccgccct tcttccgcgc actggtcgat tatgttaatc agggcaatag
      781 tgcttttgac tgccctggtc atcagggggg tgagtttttc cgtcgccatc cggcaggcaa
      841 tcagttcgtg gaatactttg gcgaaatgct cttccgctct gacttgtgta atgctgacgt
      901 agcaatgggc gatctgctga ttcatgaagg tgcgccatgt attgcacaac agcatgcggc
      961 taaagtcttt aatgccgata aaacctattt cgttttaaac ggtacttcat cttctaataa
     1021 agtggtctta aacgcattgc tgacgccggg cgatctggtg ttgtttgacc gcaataacca
     1081 taaatctaac caccacggtg cgttgctaca ggctggcgcg acaccggttt atctggaaac
     1141 cgcacgtaac ccgtatggct ttatcggtgg cattgatgcg cactgttttg aagaaagtta
     1201 cctgcgtgag ctgatcgcgg aagtcgcacc gcagcgggca aaagaggctc gtcctttccg
     1261 cctcgctgtg attcagttag gcacctacga cggtacgatt tataacgccc gccaggtggt
     1321 ggataaaatt ggtcatctgt gtgactacat tctgtttgac tccgcatggg ttggctatga
     1381 gcaatttatc ccgatgatgg ctgactgttc gccgctgttg ctggatctta atgagaacga
     1441 tccgggtatt ctggttacgc aatccgtaca taaacagcag gctggttttt ctcaaacttc
     1501 acaaatccat aaaaaagaca gccacatcaa agggcaacag cgttatgtac cgcacaaacg
     1561 catgaacaac gcctttatga tgcatgcctc caccagcccg ttctatccgc tgtttgccgc
     1621 actggatatc aacgccaaaa tgcatgaagg tgtcagcggt cgtaatatgt ggatggattg
     1681 tgtggtaaat ggcattaatg cccgcaagct gatcctcgat aactgtcagc atattcgtcc
     1741 gttcgtacct gaactggtgg atggtaaacc ctggcagtcg tatgaaacag cgcaaattgc
     1801 ggttgatctg cgcttcttcc agtttgtacc aggtgagcac tggcactctt ttgaaggcta
     1861 tgcagagaat caatactttg tcgatccatg caaactgttg ctgacaaccc caggtattga
     1921 tgcacgtaac ggcgaatatg aagcgttcgg tgtacccgcg acgattcttg ctaacttcct
     1981 gcgcgaaaat ggcgtagtgc cggaaaaatg cgatcttaac tccatcctct tcctgctgac
     2041 tccggcagaa gatatggcca agcttcagca acttgttgcc ctgctggtac gcttcgaaaa
     2101 actgcttgag tccgacgcgc cattagcaga agtgctgcct tccatctaca aacagcatga
     2161 agagcgctac gccggttata ccctgcgtca gttgtgtcaa gaaatgcatg atttgtatgc
     2221 ccgtcacaac gtgaaacaac tgcaaaaaga gatgttccgt aaggaacact tcccacgcgt
     2281 aagcatgaat ccgcaagaag ccaactacgc ctatttacgc ggtgaagtgg agctggttcg
     2341 tctgccggat gcagaaggcc gtatcgctgc cgaaggtgcg cttccttatc ctccgggtgt
     2401 gctgtgtgtt gttccgggtg aaatctgggg tggtgctgtt ttgcgttact tcagcgctct
     2461 ggaagaaggg atcaacctgc tgccaggttt tgcaccggag ctgcagggtg tctatatcga
     2521 agaacatgat ggtcgtaagc aagtttggtg ctatgtcatc aagcctcgtg atgcgcaaag
     2581 caccctgttg aaaggggaaa aattatgagt caggctaaat cgaacaaaat gggcgtcgtt
     2641 cagttaacca tactgacgat ggtcaacatg atgggctccg gtatcatcat gctgccgaca
     2701 aagcttgccg aagttgggac aatttcaatt atctcctggc tggtgacagc cgtaggctca
     2761 atggcactgg catgggcatt cgcaaaatgc ggtatgttca gccgtaaatc aggcggtatg
     2821 ggcggttatg cggagtatgc tttcggtaaa tccggtaact ttatggcgaa ctatacctac
     2881 ggcgtctcgc tgctgattgc taacgtcgcg attgctattt cggcggttgg ttacggcacc
     2941 gaactgctcg gcgcgagttt gtcgccagtg cagattggtc ttgcgaccat cggggtgctg
     3001 tggatttgta ccgtggctaa ctttggtggt gcgcgcatta ccgggcaaat cagtagcatt
     3061 accgtgtggg gggtcattat tccggtggta ggcctgtgca ttatcggctg gttctggttt
     3121 agcccgacgc tttacgttga ttcctggaat ccgcatcatg caccgttctt cagtgcagta
     3181 ggttcttcca tcgctatgac gctgtgggct tttctcggtc tggagtctgc gtgtgcgaat
     3241 actgatgtag tggaaaaccc ggaacgtaat gtgccaatcg cggtactcgg cggtacgtta
     3301 ggtgcggcgg tgatttatat cgtctccacc aacgtgattg cagggattgt gcctaatatg
     3361 gagctggcaa attcaacggc accatttggc ctggccttcg cacagatgtt cacaccagaa
     3421 gtgggtaaag tcattatggc gctgatggtg atgtcctgct gcggttcgct gcttggctgg
     3481 cagttcacca ttgcccaggt gtttaaatct tcatctgatg aaggctactt ccctaaaatt
     3541 ttctcccgtg taaccaaagt ggatgcaccg gtgcagggaa tgttgaccat tgtgattatc
     3601 cagagcgggt tggcgctgat gacgattagc ccgtcgctaa acagtcagtt caacgtgctg
     3661 gttaacctgg ccgtggtgac caatatcatt ccgtatattc tgtcaatggc ggcattagtc
     3721 attattcaga aggtcgctaa tgtgcccccc tcaaaagcga aagttgcaaa ctttgttgct
     3781 tttgttggcg cgatgtatag cttttatgcg ctgtactcat ccggggaaga agccatgctg
     3841 tacggttcca tcgtgacctt ccttggttgg acactgtatg gtctggtatc accacgcttt
     3901 gaactgaaaa ataaacacgg ttaaaaaagc aaagtaaaaa agggcgatct tgcgaccgcc
     3961 ctttttttat taaatgtgtt tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt
     4021 ctttctcaat ctgcttgcga tgttcttcac cgaggaagct ttcgcagtag atcttatatg
     4081 cgtcttccgt gcctgacgga cgcgcggcga accagccgtt gtcagtcatc actttcagac
     4141 cgccgataga agcaccatta cccggtgccg ccgtcagacg tgcggtgatc gggtcacctg
     4201 ccagggtgct ggcgctcacc atttccggag acagcttaga caacgccgct ttttgtgcgg
     4261 aagtcgcagc ggcctgcaaa cggttgtagc tcggcgcacc aaagcgtttt gccagttcgt
     4321 tgtagtgttc ctgcgggttc ttaccggtga cagcggtgat ttccgccgcc agcagacaca
     4381 taatgatgcc gtctttatcg gtggaccacg gcgtgccgtc gaaacgcagg aaggaagccc
     4441 cggcgctctc ttcgccgcca aagccgaaac tgccgtcgaa cagaccatca acaaaccatt
     4501 tgaatcctac cgggacttct accagtttac ggcccaaggc gttgaccaca cggtcgatca
     4561 tcgcagaaga aaccagcgtt ttaccgacag caacatcttt gccccactgc ggacgatgct
     4621 ggaacaggta gttgatcgcc accgccaggt agtggttcgg attcatcaaa cctgccggag
     4681 tgacgatacc gtgacggtca taatccgggt cgttagcaaa cgccagatcg aacttatcac
     4741 gcagtgccag caggcccgcc atcgcacact cggaggagca gtccatacgg atcgtgccgt
     4801 ctttatcaag gtgcataaag cggaaggttt gatcgacctg atcgttaacg atggtcaggt
     4861 tgaggttgta atactcgcca atacgcttcc agtattcgat accggaaccg cccagcggat
     4921 caacgccaag cgtcaggccc gctttctgaa tcgccgccat atcaacgata tcggccagac
     4981 cttccacaaa cggctgcacc agatcctgct ctttcacatg accggatgcc atcgcttcgt
     5041 cgagagagat acgcttcacg cctttcaggc catcggccat cagtgcgttg gccctgtctt
     5101 ccaccacttt ggtgacgttg gtatcagccg ggccaccatt tggcgggttg tacttgatac
     5161 caccatcttc cggcgggtta tgggacggtg taatcacgat accgtctgcc agcgggccac
     5221 cttttttatt gtgaaccagg atggcattgg aaacggcagg cgtcggagtg aatccattgt
     5281 tttcctgcac aatgacatca acgccgttcg ctgccagcac ttccagtacg gaaatgaacg
     5341 caggctcgga cagggcgtga gtatctttac ccacatagca agggccagtg atgccatttt
     5401 tcgcacgttc ttcagcaatt gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt
     5461 ggcgcgctgc actgccacgg tgaccggaag taccgaattt caccgcgtgc tccgcattcc
     5521 ctgcttctgg tttcagtaca taatattgcg ccgtcagttg ggcgacgtta atcaaatcac
     5581 tctgttgtgc aggttggcct gcacgattgt ggattgccat tgctttgtcc tttgtctgca
     5641 acgttttaga tagttccgcg aaccttctca atcaattccg ccgggaattg catcgactgc
     5701 atgatgtgtt cgatcatgct gcatttacgg ccggtgttgg tgttggtgat cacccaatac
     5761 ggcgtgcctg gcacatgttt cggcttggtc tgattaccat ttttcagcag cgtttgttca
     5821 tctgccgcaa agtaaacgcg tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc
     5881 tgggcgtcaa gagaatatag tgtagacaac agcagcataa agcgattgac cgctcgcttt
     5941 tgctctgcgt attcgtccga aagcagaagt tcacgcattg cgcgaacctt gtctttaatc
     6001 gttttgaccg gcttcgcttc gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc
     6061 ggagcagcag gctgtgatga ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg
     6121 ctctcgccga tatgcttagt gtggctggca atatagctgt agagttcatc atcaacttca
     6181 atcgttttca tcttaatcca gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat
     6241 gaataaatcc cggcagcgga tagcgtcaag cctggcttgc agaaaaactc ggaataaacc
     6301 ttatttgttg cagccctgtg gcacaatggt caacataata ccctaacccg acaacgcgaa
     6361 aaaaagaact ttgccatgaa attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat
     6421 aattctccca tcgttcttgt ccatggtctg tttggtagcc tcgacaacct tggcgtactg
     6481 gctcgcgatc tggtaaacga tcacaatatc atccaggttg atatgcgtaa ccacggtctt
     6541 tcaccgagag atccggtaat gaattacccg gcgatggcgc aggatcttgt tgatactctg
     6601 gatgcacagc agatcgacaa agcaacattt atcggtcact ccatgggcgg taaagcggta
     6661 atggcactta ctgcactggc ccccgatcgc atcgataaac tggtggcgat cgatatcgca
     6721 ccggtcgact atcacgtacg ccgtcatgat gagattttcg cggctatcaa cgcggtcagt
     6781 gaatcggacg cacaaactcg ccagcaagca gcagcaataa tgcgccagca tcttaatgaa
     6841 gaaggggtga ttcagtttct gttgaaatct tttgttgacg gggagtggcg ctttaacgtg
     6901 ccggtattgt gggatcagta tccgcatatt gtgggttggg agaaaatccc ggcatgggat
     6961 caccctgccc tgtttatccc tggcggcaat tctccgtatg ttagcgagca gtaccgtgat
     7021 gatttactgg cacaatttcc acaggcacgg gcgcatgtga ttgcaggcgc gggtcactgg
     7081 gtccatgctg aaaaaccgga tgcggtatta cgcgctatcc gtcgctatct caatgattaa
     7141 cgcaactgat taaaaaccca gcgtccgcgt cgctttccgc gcgtgggcgt tggcgtgact
     7201 catcgcgctg atgtattatg gcgcgctatc catccgggca gaggcctggc tgattgtttc
     7261 ccccgaagtc accaagatca tggccaaaga acaaacggac cgtacgacat tagatctgtt
     7321 cgcgcacgag cgtcgaccgg gacgaccgaa aactaatccg ctttcgcgcg atgaacagct
     7381 gcgtattaat aaacgcaacc agctaaaacg cgacaaagta cgtggcctta agcgtgtcga
     7441 actgaagctg aacgcggaag ctgtcgaggc gctgaacgag ctggcggagt cgcgcaatat
     7501 gagccgtagc gaactgatcg aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca
     7561 gggcatcgtt taaatttcca ctttcatgta gcacagtgtg cagtcctgct cgtttgctga
     7621 ttccgcaaga ctgcctgttc tgctatgatt gcctttatcc gtgggcaatt ttccaccccc
     7681 atttcaataa gtttcaagag gttatttcac tcatggctat cactggcatc tttttcggca
     7741 gcgacaccgg taataccgaa aatatcgcaa aaatgattca aaaacagctt ggtaaagacg
     7801 ttgccgatgt ccatgacatt gcaaaaagca gcaaagaaga tctggaagct tatgacattc
     7861 tgctgctggg catcccaacc tggtattacg gcgaagcgca gtgtgactgg gatgacttct
     7921 tcccgactct cgaagagatt gatttcaacg gcaaactggt tgcgctgttt ggttgtggtg
     7981 accaggaaga ttacgccgaa tatttctgcg acgcattggg caccatccgc gacatcattg
     8041 aaccgcgcgg tgcaaccatc gttggtcact ggccaactgc gggctatcat ttcgaagcat
     8101 caaaaggtct ggcagatgac gaccactttg tcggtctggc tatcgacgaa gaccgtcagc
     8161 cggaactgac cgctgaacgt gtagaaaaat gggttaaaca gatttctgaa gagttgcatc
     8221 tcgacgaaat tcttaatgcc tgatgtgatg cggcgtagac tcatgtctac gccgtattaa
     8281 tagataatgc caatcaaaat aattgctaca aatttgtaac ttttgctgtt gtacctgtac
     8341 aatgtcccgg tgttcaagtg gccttgccgt tgtaaatgta agctgtgcca cgtttttatt
     8401 aacaatattt gccagggact tgtggttttc atttaggcgt ggcaattcta taatgatacg
     8461 cattatctca agagcaaatt ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg
     8521 acagattccg catgactgat aacaataccg ccctaaagaa agctggcctg aaagtaacgc
     8581 ttccacgttt aaaaatcctg gaagttcttc aggagccgga caaccatcac gtcagtgcgg
     8641 aagatttata caaacgtctg atcgatatgg gtgaagaaat tggtctggct acggtatatc
     8701 gcgtactgaa ccagtttgac gacgctggta tcgtcacccg ccacaatttt gaaggcggta
     8761 aatccgtatt tgaactgaca cagcaacatc accacgatca cctgatttgc ctcgactgcg
     8821 gcaaggttat cgaatttagt gatgattcca tcgaagcgcg tcagcgtgaa attgccgcaa
     8881 aacatggcat tcgcctgact aaccacagtc tctatcttta cggtcactgt gccgaaggcg
     8941 attgccgcga agatgagcac gcgcacgaag gcaaataagc cagcctgaac gagaaaagcc
     9001 aacctgcggg ttggcttttt tatacaaggg aaataagaaa agcgagagtt acagctctca
     9061 cttatttgtt attactgcgt atttcctgcc agaccttatc acaatcggct ttcaccgcct
     9121 gatcatttcc ggtttgcgtt gcacgcagac actgctgata atccagtacg cgtacacttt
     9181 cctggtcggc aaactgctga tgttgtttct ctttcttcag cacgtttaac acgctctggc
     9241 aggcttcaat tttttctggc gaaccttgtg cggtgttgat acaggcgctg tatgcctctt
     9301 tcagacgact gtcttcctgt ggcgcggttg attgcgcaca agctaccagc cccgatgcca
     9361 ttatggcgat gagaatcagt tttttcatag caaattcctc aaacgtgcgg caggcacaaa
     9421 acctgccgcg tcgggcatca gaagatggtg aatggtgcga ttaccataaa tttcacgtca
     9481 cgctcatcct ggaagatgtt accgtaaccg ccgccccagc ttgggatgtc agagtggttg
     9541 tcgtattcgg tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg
     9601 tagaccgcat ccaggctgta ggcagactct tcaatagtcc ggtttttgtc gtagtacgca
     9661 tccggattgc tctgccaggt cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg
     9721 atggcgaagc ctggaagatt ccagtttttc aggtcataca tcgcaccgaa gaagaccgct
     9781 ttttcgccgt tggcgttgaa gtcggaacgg ttatcccacc agatatccag gcggccgttg
     9841 gaggaagcgt aggttggagt catacgttgc aggaagtatc cctgctgacc gtcagcctta
     9901 acccaggtgc cttcgaggcg caaatccact acgtcagccg cccggtaacc aaaggtcaac
     9961 gcttgcagcc aggcggtgcc gtcataaagg tcgttgacgc tgcgatcgtc aactttatcg
    10021 cgcgtaccat agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag
    10081 ctggctttgg caaaatattg atcgatatac ccttccgcct gaccaaatgc cgcttccagt
    10141 acgaagttat ttttgaagtc gtatttcgca ccgatggagt gcaggtaatc aactttggtg
    10201 gttttatcgt tctgataaaa ctcatccatt tccagatgcc acggcgcttt gtattcgttg
    10261 gtccacatgt aggagaaact caaggcacca gcatcgccgt aatcaaaatt cgccccggct
    10321 tccgccccct gataagtacc tggcataaag ctccagtgcg gcgctaacag cgtttgacca
    10381 gttggctgaa tgtaacctgc cctcgcccaa accggaccgt atttaaattt ggccgcagct
    10441 ttatacaggc ttataccgct tttgtcgccg gaccagtctt cgtcataggc tttattactt
    10501 tttgaaaacg cgatttcgct cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc
    10561 gtaaacgcag caatatcaag gccgaacata tcagcagcat aaccggactg aaaatcgagg
    10621 ttggcattcc aggtggagtg agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct
    10681 ttacggtcgc gttcacgctg ccagtaatat ataccgccgg ttaaggttga atcgtcgatg
    10741 aatccttcgg cgcgagcctc cggcattgcc ataaagcccg acattgctgt aacaccggcg
    10801 atagccagcg ccagcgtact acgtttgcca ctaaacgtac gcatgggtta atcctctttg
    10861 acgtataaat tgctgcacca aaggtgaaca gcgaaaaaat aaaaattaaa aaagtagcgt
    10921 tgagaaaagt ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct
    10981 ctttctgtaa ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta
    11041 caaggcttaa gtttgcggtt gaaaggcttg cagaaga
//
LOCUS       DAZFJF010000136        11071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFJF010000136 DAZFJF010000000
VERSION     DAZFJF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.137"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..669)
                     /gene="fimB"
                     /locus_tag="ACHKAU_004099"
     CDS             complement(67..669)
                     /gene="fimB"
                     /locus_tag="ACHKAU_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO5533062.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2125..2841
                     /gene="nanC"
                     /locus_tag="ACHKAU_004100"
     CDS             2125..2841
                     /gene="nanC"
                     /locus_tag="ACHKAU_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO5533063.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2861..3967
                     /gene="nanM"
                     /locus_tag="ACHKAU_004101"
     CDS             2861..3967
                     /gene="nanM"
                     /locus_tag="ACHKAU_004101"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO5533064.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4032..5012
                     /gene="nanS"
                     /locus_tag="ACHKAU_004102"
     CDS             4032..5012
                     /gene="nanS"
                     /locus_tag="ACHKAU_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5533065.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(5020..5670)
                     /locus_tag="ACHKAU_004103"
     CDS             complement(5020..5670)
                     /locus_tag="ACHKAU_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5533066.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            5807..5950
                     /locus_tag="ACHKAU_004104"
                     /pseudo
     CDS             5807..5950
                     /locus_tag="ACHKAU_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(6614..6742)
                     /locus_tag="ACHKAU_004105"
                     /pseudo
     CDS             complement(6614..6742)
                     /locus_tag="ACHKAU_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(6718..6867)
                     /locus_tag="ACHKAU_004106"
                     /pseudo
     CDS             complement(6718..6867)
                     /locus_tag="ACHKAU_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(6952..7149)
                     /locus_tag="ACHKAU_004107"
     CDS             complement(6952..7149)
                     /locus_tag="ACHKAU_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5533067.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(7161..7649)
                     /locus_tag="ACHKAU_004108"
     CDS             complement(7161..7649)
                     /locus_tag="ACHKAU_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5533068.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(7646..8020)
                     /locus_tag="ACHKAU_004109"
     CDS             complement(7646..8020)
                     /locus_tag="ACHKAU_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5533069.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(8110..8478)
                     /locus_tag="ACHKAU_004110"
     CDS             complement(8110..8478)
                     /locus_tag="ACHKAU_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5533070.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(8641..8862)
                     /locus_tag="ACHKAU_004111"
     CDS             complement(8641..8862)
                     /locus_tag="ACHKAU_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5533071.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8925..9401)
                     /locus_tag="ACHKAU_004112"
     CDS             complement(8925..9401)
                     /locus_tag="ACHKAU_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5533072.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(9417..9902)
                     /locus_tag="ACHKAU_004113"
     CDS             complement(9417..9902)
                     /locus_tag="ACHKAU_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5533073.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9957..10775)
                     /locus_tag="ACHKAU_004114"
     CDS             complement(9957..10775)
                     /locus_tag="ACHKAU_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5533074.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRVLWVMAETLLESLR"
     gene            complement(10875..>11071)
                     /locus_tag="ACHKAU_004115"
     CDS             complement(10875..>11071)
                     /locus_tag="ACHKAU_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5533075.1"
                     /translation="FSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRRWRCRNS
                     EPDAGKVLNTRLASDGLLRQ"
BASE COUNT         3002 a         2541 c         2667 g         2861 t
ORIGIN      
        1 aatcattttt gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa
       61 ctatctctat aaaacagcgt gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa
      121 acgccctgca ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata
      181 atcctggata agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatggcg
      241 taacatgtgc ggatgaatct ccagtgacaa cccggcatta ccaccggaag tcgagataat
      301 atggtaaaac tgttgccgag aaagcggatt tcccttacgc gataaaaata cccattcgct
      361 ctcagcatgc gggtacgaag tacggatact caaccagttt tttaaagcct gaacttcttt
      421 gttcaatagc gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt
      481 tgccttaaga tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg
      541 aataaaacaa agcaaagtca gacaataatt acgtgctgca tgaggcccgg tatttgctgc
      601 tttaaggagt gattcgattt cactatgggt caggaagttc ctttttttgt tatcagcctt
      661 attcttcatc gtttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat
      721 aatccaacat ttttatcctg attatgttta taaaagcgaa cgtttgctta ataactaacg
      781 taagtggacc agttcttctg agtgaactta aatggagtag caacagttac ttataataac
      841 gttgccatac aaacgctatg ctcttgcatg ctatgtacct ttatatattt atcaatcaga
      901 tacgaatcta ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga
      961 tatgttctgt aacaagtaaa atcaacataa aaaattcagc aaaatttaat attcgttcaa
     1021 ctatccgtct cacatatgac attattagac aactccattt gccacaaaat ggctatttca
     1081 tggaacgcag taatttctta gtagctatta tcaggctaat tcttaggtcc ctagcgatta
     1141 ttcctggctg gttcgtcagt ttagttcatg agattgttac attccttttt atcctggaga
     1201 tatgggggaa agcaggagat gattttttca tctgcatccg ttggtaaata tggctttggt
     1261 cactgcgact taaaccttcc tcaatcacag ctgttcgccg ggctattcgg caaacaagct
     1321 aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc tctccagaga gacttcagga
     1381 gtgaccagct acaagccaca aaccaggaaa catatttcat gatgagaatt atgctcagcc
     1441 tgatggcggg taaacactta ttttcaccac gcccactgag ttatcttgca aatctttcct
     1501 tgtttatact ctaaataaat tacttataaa acagataatt aaataacaac ccttggtttg
     1561 tgaaggcctc cgcaagtgtt cgtttggctt cacatggcat cttcttctta gaaaaatatc
     1621 gacatatttt gtgacacgaa ttgcaaatct ggttttgttg tatggattgc gtgatttttg
     1681 atctggtata acaggtataa aggtgcacca agatagtcaa tgagacaggg catctcgcaa
     1741 tctatggcaa acatcacttc agttctttca catcgggtga tgaaaatgca cttcagtctg
     1801 aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg taaaatggat
     1861 tgagtaagtg atatagctta cgaacattca aatcaattaa acatcagaag agattttata
     1921 ctcaggtatt taatctggct ctctgtttat ttaaataatg tgaaaagaga tttttcacag
     1981 gagaccttat acaaaaaata taaaatacag ctaccggttg ccaaagacac tataagcctg
     2041 gcaaaaaaat attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct
     2101 gtttcctaca aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta
     2161 ctgtgctttt cgtcaccatt aatttctcaa gctgcgacac tggacgtacg tggtggatat
     2221 cgtagtggaa gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga
     2281 tggtgggcaa gtatggaaag taatacctgg aataccattc atgataataa aaaggaaaat
     2341 gccgcactca atgatgttca ggttgatgtt aattacgcga ttaaacttga tgatcaatgg
     2401 acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac
     2461 gtaaaactgt cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac
     2521 tggaaagctt accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt
     2581 tgggatggat atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg
     2641 ctatacagca aacagaacga ttatcgctat gcaaaccata agaaatgggc gacggaaaat
     2701 gcatttgttc tacaatacca tatgacgccg gatattacgc catacataga atatgactac
     2761 cttgaccgtc agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt
     2821 ggtgtgtcat ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc
     2881 gcttgctatc atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt
     2941 gccatttaaa agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag
     3001 cgcaggtacg gcatggtaca agctggatac acaggccaaa gataaaaaat ggaaagcgtt
     3061 agctgcattc cctggtggac caagagatca agcaacctcg gcatttattg atggcaatct
     3121 gtatgtgttt ggcggcattg gcaaaaacag cgagggcttg actcaggtat ttaatgacgt
     3181 acacaaatac aaccccaaaa ccaatagttg ggttaaattg atgtcgcacg caccgatggg
     3241 catggcgggt catgtaactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa
     3301 ccagaatatc ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc
     3361 tatagataaa atcaacgctc actattttga caaaaaagca gaagattatt ttttcaataa
     3421 gtttctgttg tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg
     3481 gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg
     3541 cgaagccaaa ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa
     3601 tttaaaatgg aataagcttg ctcccgtctc atcaccagat ggcgtagctg gcggttttgc
     3661 ggggataagc aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga
     3721 aaattaccag aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga
     3781 tattcatctt tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc
     3841 ctacggagta tcattgccct ggaataatag tctattgatt attggcggtg aaactgcagg
     3901 cggcaaagcg gtgacggatt cagttttgat ctctgtgaag gataataaag ttacagtaca
     3961 aaactaacgc ttcagggccc cggtaaaggg gccctgctat caatttgtca tttcaattac
     4021 gcgaaattta tatgaacgca ataatatcgc ccgattatta ctatattctt accattgctg
     4081 gtcagtctaa tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc
     4141 ctcatcccag aatcaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat
     4201 gtcactttaa cgacatgatt ccactgactc actgcccaca cgatgttcag gatatgcagg
     4261 gttatcacca tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac
     4321 tgcatatagc acggaaatta ctgcccttta ttcctgataa tgcaggggtt ctcatcgttc
     4381 cgtgttgccg tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc
     4441 acggagccag ccatgatgct tgtcgttggg gaacggatac tccgctatac caggatttag
     4501 tcagcagaac gcgagccgca ctggcaaaaa atccgcataa caaattcctc ggcgtatgct
     4561 ggatgcaagg cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta
     4621 atcatatggt tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata
     4681 ttactgacgc accgtggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc
     4741 atgcgtatga agttatttat ggcaattatc aaaataatgt tttagccaat attattttcg
     4801 tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg
     4861 atttaagcac gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac
     4921 tgcgaagcag tcatttcagc acggcagccc gtcgggggat tatttctgac aagtttgtag
     4981 aagcaatttt gcagttttgg cgcgaaaggt gagcgttcat taatttgatt tattcgcatc
     5041 catcagcgct tttaactgct ccggcgtaaa cggtggtttg cgtcggtgca acatcgcatg
     5101 gcaattaggg tacacaggca gcaaatcatt aactggatcc agctggtaat cctctttaat
     5161 ggctgacagc ggcaccaggt gatgcacatg gataaagcct tcagcgatat caccatagat
     5221 cttcaccaga tcaacaccgc atacgttgca ctggcatcca tgatgttcga ccgctttatc
     5281 tcgggccttt ttatcgcgtt catagcggtt tactgtcacc tttatcgccg caccttcaag
     5341 gtatgtctcc tgcgacggca actcatccgg gaacagaacg tcggctatgg gagccgcagc
     5401 ggctttatcc agatgaatat gaaatccctg ctctatgagt agcttgacac atttagattt
     5461 tattccgccg gtgaaatccg caggggtaaa ttgcgtcccc gtcatcaatg tcgctgcgat
     5521 gccagcaatc gccttcgagg ggtaacgtcg gccatgatat tcaatctcat acaaccgcgc
     5581 aggctggaac tgatgagttt ctcccgcatc ccataaatgg atagcttcga tgatataccg
     5641 ccgcggaaga tgatcaggga gtttattcat ggagaatgtc cttatacgtt ctcagaacag
     5701 gcagcaaaaa aacaaatgag atcaaggtaa tatatcttca cttgcaggct tgaacagcag
     5761 aaaatttgcg agcatgatcg caattatttc actgccaggg atatcaatag cttccagtaa
     5821 atcgctacag caggcaattg ccaatataaa aatctggcac aagggtgaac agcgcgcgcc
     5881 gcataagcca ttgttattgc tatacgtatt agcgggatac ctgaatggac atccgcgcct
     5941 tttcgattat tgtagtggaa cagtctacga ggatgcagtt cgaagtgagt aagtaaaggt
     6001 tgcgggaaac gatcaatgcc gccatacaat gattttttag gatcaacaat gagtaataaa
     6061 aactacgaaa gtcaccgcaa agcgattgtt agtaaaggta taccacccgc tctattaaat
     6121 aggctcacca attcggatgt tcaggtgatc aataccttct taacacgagt gagcaaactg
     6181 gaactatctc aacaagagaa agactggatc ataaaaatca tctctatggt ttagacaatt
     6241 tcagccttgt agcctttcaa gggttttgtg tgggtacaaa cattatcgaa tcggggtata
     6301 gccgcagctg tagctgtatc cacatcagta agttgatgta ggttgactta ggttgagcga
     6361 aaaatagcga aaagccttgt gtggcgcgga tttcagtcat aaaaaaacgt ccgttgatgt
     6421 ctattgatgt tccgatggtg cgaaggtgcg aaggtgcgaa ggccggactc aaacatgtga
     6481 cttaacctca tgatttaaaa ttgttaataa taaacaatat catcttatac caacacagat
     6541 accaacacga aaaatcactg gttttcggca tcgaaccagg acaatcagct cctgattgag
     6601 ataccgagct ctgttactcg tcatcttcct cgacaggctt cattgcatca agtagtactc
     6661 gcaccatcgc gatagaacca atccggataa aagcatcttc tccatctata tctgttaact
     6721 gatatcgctc acttgatgcc ggataattgt gtcgatttgc agcagtgaac tctttcgtac
     6781 agccagaatg cggcggatgc tggttgagca gataacgtcc gtattctcgt tgaccacttc
     6841 cggacggtag gctgtgatat cagacataat cgatattcct ttttgcagat aaatgaaaat
     6901 gccagccctg ttcaggctgg cgtcagtgga atgaagtctg gtgagtaaga ctcaccggtt
     6961 ctggtgctga agctgcagtt ggcgcagggt acggatatcc tctctggcct gttcgataca
     7021 gggcaacagt gctgctgaat ccgttttatc ctcattgttg tcaaagataa ttcccgattc
     7081 gcagtcgata ttgtcctgca gccacgcaat cagaatatcc agtgcggttt ccgtggttaa
     7141 tgatttcatg ttgttcctgg ttaccagttg aaagtgggta aacccggcag gcattcagcg
     7201 ctggcatcca gatgaatgag actgacgcca taacgtcggg taagtgtgat aaccagacga
     7261 cggaacgttt ttgacagccc caggcgttta agccgcagaa ccggatatga ccacgcatcg
     7321 gtacgtaaca gatatccgct acctgtgaaa tgaatccatt ctgattcacc aacgtcactg
     7381 gtctgatgcg acagcgagta cagccaggcg ttatcctttt ccgtgatatg tgcggtactg
     7441 cagcgtatgc cggcaagggt tgcaaacggt ggtagtgtgc aggttgactg ttggtcggat
     7501 tcctccacca cgcggagtga ataaccgttt tcagcgacct tgttaatcag ttcagtgaga
     7561 ttaacaccat cgacgtcaac gacaattcgc cccatgttca gtgcctgcac attaattctg
     7621 tcggcttcca gcatcaggga aagtttcatc gtttctccgg atgcttaccc agggtaatgt
     7681 tatttaccgt tctgtaattg tcgcgggtca tcaggccggt cgccctgcga gcccggagga
     7741 tatcaatact gtttattaac tgagagcggg gacaagtgct gaatcccggc tggtcggtac
     7801 gcaccagcgc gtatttttcc acgagaaaat tcaccgcatc acacagtgaa atgccagctt
     7861 caatatgctg ctcaatcaca cgttcatcgg cgaacggtgt gtcattcagt gtaaggccgt
     7921 aatgctggcc cagtagtcgg gagagcagag tctgccagat ggtgacggga gacgggcagc
     7981 tcgatgcctc ccgtacgtgt gtatcgggta atgttttcat actgaaggtt ttcctgatat
     8041 gcagagatga aaaatggaaa agtggcgtgg tgaaagcacc aggccggagc agaaggttat
     8101 tccggtggtt tactttttcg tttcgggcgt cggataaaca gccagataaa cgtaaccacc
     8161 gctaccgaga gtgtcagctt tgcaggtcag accttttgca tacagcgtga cggtatgctg
     8221 atggcgggga ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg
     8281 aaaggcctgg tccaggtggt acgcatctgc atcgctgaac aggcctctga taccggcgcg
     8341 gtcggcaagg taatgcaggc ggttaccctc ctgcaccaga cgggcgccga aacagggcgt
     8401 cactgtgcag ggtagtcccc accagggatg gtcgtcattg tcatcgggat gcgttgtccc
     8461 ggaaagtgtg tctgacacga taaaatcctc acagaaaatc ggtgaatgat agtttaacga
     8521 tgacgggtca gccggcgcgc agaccggctt cggtaaaggt gtattcgttg atgagcagga
     8581 cctcatccac agcggcgtct gagctcagcc agtcgtactc gttttccggg tgacggaaca
     8641 tcagttcagg gtgacgctca tcagccggac atacggacca aaactgtcct tacggcgttc
     8701 tgcaaacacg gccagcacac cgggaatatc ctgcacttca cgaccggtat acgcttcagc
     8761 actgccgtgc cagcggtatt tgccggtaca gaacggaaaa agacgggatg ccggatgctg
     8821 acggtgaata cgcatggctt cgccacgggt gatgattttc atagtgggat acctctgaag
     8881 acagaagata aaagtgaaaa caggtgtgat gtggttgtga cgggttaaag cagaccatgt
     8941 tcggcaaagg agaaaacctg gctgccaccg actatcagat ggtccggcac ccggatatcc
     9001 accaggccca gtgcctgtac cagacgttcc gtgataaggc ggtctgcctt gctgggtgtg
     9061 acttcaccgg acgggtgatt gtgtgccagc accacagcag cggcattgtg atacagggcg
     9121 cgtttaatca cttcacgggg atggacttcc gtgcggttga tggtgccggt gaagagggtt
     9181 tcaccggcaa tcagctgatt ctggttgttc agatacagca cccggaactc ttcacgctcc
     9241 agtcccgcca tgttcagaat cagccattct cgtgccgcac gggtggaggt gaaggccacg
     9301 ccgggttcat gaagatggcg gtccagggtt ttcagggccc gttgaatgag gctgcgctcg
     9361 ccgggcgtca tctctccggg cagaaaggaa agctgttgca ttgtacttct ctccattcag
     9421 tcgatgatgc gcataatggc gctgcattcc ggatgctgca gggcgtaatc ccgcagccgg
     9481 taataatgga ccgtcatggc ataacactcc gtacgacagg catgatgact gtacgtcatc
     9541 agacaggcag caatgccggc agcttccgga ctcatttcag cgcggttacc gttcatggca
     9601 ttgaacagta cccatgtttc gtcatcatcg tcatccggtt cgggtgccat aaatgccccg
     9661 ccgttgttca gggtgtacag attccagata ccaccgcagt agtcttcaca cagacggtcc
     9721 atccagccga agacacgggg ctccagggtc acccactgtg gaatgaggcc aaagtgctgc
     9781 ggccagaagc tgatgcgctg ttcatcaggg acgagggtgg caaccaactg aggctggtca
     9841 ttccctgatg cagcggttac ggaaacagaa gatgtggtgg aattatgtga aatggttttc
     9901 atgaaatgac tccttaaagg acaaataaat gcgtggtttt cagggtaatg gctgaatcag
     9961 cgcaggctct ccagcagggt ttccgccatc acccacaata cgcggttgag cttaatgtca
    10021 gtgtcaatgc tgtgaatggc acgggtatgg atacgttttc ctttagcgct gcgacctgaa
    10081 attccgcctt tcagcatatt ctcctgaatg gtctgataag cactccacag gtccttaccg
    10141 taatcctccc ggcgtcgtgg cgtcagaatg tcggcggtgg tgacgggctg atgttcgtca
    10201 ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg cggcagaatc
    10261 agcgactgca tggcatcacg cttttcctca atccggtcaa acacgcccac cacctcgtaa
    10321 gccccttcaa tgactctgtc caccacattt ccccggtgcg gaacacgcac ttcccccaga
    10381 gactgaccac agacgcatcc gttctggcag acgaacctga agtaacccgg cagcatctgg
    10441 tagctggagg taccgtcatg agagttgagc agaataattt cagggacatg ttctccgttt
    10501 atctctccgg cccgccgcag acgcagcatg tgttttgtgt atccccggcg gcccgggtcg
    10561 cgcacacggg tctggcaggc gaagaatggc tgaaagcctt cccgctgcag gctttccagt
    10621 acggtgatgg tggggatgta cgtatagttc tggcttcggg acgtgtgttt atcttccccg
    10681 aaaatccccg gcacatagtg catcagttct tcgtgtgtca gcggacggtc acggcgtatc
    10741 tggttcgcat aaccaaaacg actggctagt cgcataattt gctccttatc ggtggttaag
    10801 atttactggt gtaataaatg aaaaagccac gtctcccgga gaagacgcgg cctgatgggt
    10861 gaaatgaatg acgtttattg tctgagaaga ccgtcactgg cgagccgcgt attaagcacc
    10921 tttccggcat cgggttcaga attccggcag cgccagcgtc gctgaccttc cgcgttgcgg
    10981 ataacccgac ggaaatgcat tcccgggtca tcctggataa acacattgcg gtaagcggtt
    11041 atgacagcaa ccgcctgccc gtatgagaaa g
//
LOCUS       DAZFJF010000137        10909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFJF010000137 DAZFJF010000000
VERSION     DAZFJF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.138"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="ACHKAU_004116"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="ACHKAU_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO5533076.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHGIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="ACHKAU_004117"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="ACHKAU_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO5533077.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFYAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="ACHKAU_004118"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="ACHKAU_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO5533078.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="ACHKAU_004119"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="ACHKAU_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO5533079.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHKAU_004120"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHKAU_004120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO5533080.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHKAU_004121"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHKAU_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO5533081.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHKAU_004122"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHKAU_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO5533082.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHKAU_004123"
     CDS             complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHKAU_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO5533083.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHKAU_004124"
     CDS             complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHKAU_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO5533084.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2722 a         2846 c         2697 g         2644 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt atttgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acggcatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagtt gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc tactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga tccaagtcct ggtggttctt acgcgtacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggccgttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgta
     1681 ctttctacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcttcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc
     1801 tacgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1921 ggcacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc
     1981 agcatttcac caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggtca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggctgtt actaccattg ccttcctcta ctgcatttgg
     2161 gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct gatggtaatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctgcataaaa acccgtatcc cttagatgta
     2281 ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggataaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactac tgattttgct ggtcgccgca ctgttttata aagactacgc
     2941 ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctaccgctgg tgcgaattgg
     3061 tgaagacgcc caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtagc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcaatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggattga ttctggcggc gcaaactgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atacaccctg
     4261 ccagtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgctgg gctggatgtc
     4321 ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcattttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtgcgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcactgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttaaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacgacttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg
     5581 gcagttgaag atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atattatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacaggg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tgtttggtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcgac atgctgctgg aacgccggag tttcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccactacgcc agcacccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggtgagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gcccgccaag cggtcgaatc
     7261 agaaagggaa cggagaaagt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aattggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgagcatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg acaggcatcg ccaaatgccc
     8101 cccaacgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgatc acctgcctgc accagtaggc
     8221 ctcgttgcgt taacgccttt tccttccagc ggcaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca
     8341 gcagcgccag atgctgcgcg atctgcgtca gtgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccaatgtc agacgcaggc taagcggcgc atttaacatg ccgccgttcg
     8461 tccatgtctg aagctgcacg cgcgcaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg
     8581 gataaaccga ctccagcacg cgaccggggc cgtcaagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtccgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccagacttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat aaagatcgac ttctgcgctt aaagctcgtt gcaacacact
     8941 gtaatcaacc gtctctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcttc
     9061 ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg aagtggggaa ctgcgccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt ccagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcgggtacga atctcgctta acggctccga atcatcctga aacaacgcgg ctaactgtgc
     9361 attcaccgca tcttgctgtt gacgcagaaa gtggttggcg gaggccaagg ccagctcgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cggcagatcg
     9601 cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc ggtggtaact taccgttgtt ggctaactca tggcgcgccc gattcgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaactgacc gggataactt tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattttt gaattaaacg gtcgattggc gcgacatgtg aaaaatgcag
    10501 taccggttct ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
    10561 attacgatta ggcaaaacct ccgtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatggta ccgacaatcg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcgtgacgtt cggcgatatt aggctgactc cagggtaaag ccagttgtgg
    10741 cgcatcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga
    10861 aaattcttcc ttaaagcaaa ttttgttact tttatttagc cagattgtt
//
LOCUS       DAZFJF010000138        10888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFJF010000138 DAZFJF010000000
VERSION     DAZFJF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.139"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(116..2812)
                     /gene="mgtA"
                     /locus_tag="ACHKAU_004125"
     CDS             complement(116..2812)
                     /gene="mgtA"
                     /locus_tag="ACHKAU_004125"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO5533085.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(2953..3006)
                     /gene="mgtL"
                     /locus_tag="ACHKAU_004126"
     CDS             complement(2953..3006)
                     /gene="mgtL"
                     /locus_tag="ACHKAU_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO5533086.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            3188..4138
                     /gene="treR"
                     /locus_tag="ACHKAU_004127"
                     /pseudo
     CDS             3188..4138
                     /gene="treR"
                     /locus_tag="ACHKAU_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            4257..5678
                     /gene="treB"
                     /locus_tag="ACHKAU_004128"
     CDS             4257..5678
                     /gene="treB"
                     /locus_tag="ACHKAU_004128"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO5533087.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            5728..7383
                     /gene="treC"
                     /locus_tag="ACHKAU_004129"
     CDS             5728..7383
                     /gene="treC"
                     /locus_tag="ACHKAU_004129"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO5533088.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            7777..9915
                     /gene="nrdD"
                     /locus_tag="ACHKAU_004130"
     CDS             7777..9915
                     /gene="nrdD"
                     /locus_tag="ACHKAU_004130"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO5533089.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            10074..10538
                     /gene="nrdG"
                     /locus_tag="ACHKAU_004131"
     CDS             10074..10538
                     /gene="nrdG"
                     /locus_tag="ACHKAU_004131"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO5533090.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(10583..>10888)
                     /gene="cybC"
                     /locus_tag="ACHKAU_004132"
     CDS             complement(10583..>10888)
                     /gene="cybC"
                     /locus_tag="ACHKAU_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5533091.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
BASE COUNT         2647 a         2895 c         2806 g         2540 t
ORIGIN      
        1 attcctgcaa ttgattgaat ttgtaggccg gataaggcat ttacgccgca tccggcatga
       61 acaacgctca ctttgtctgc aatctgaatc ggggctatcg tgcccagttt attctttact
      121 gccagccgta acgacggcta tagaaccctt tcaccaactg ggttaacgtc atataccctg
      181 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagc tgcagataac
      241 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
      301 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtacgg atcatatgca
      361 caatcagcgt ttgcgacagt aagcccacca caaaccatcc cgactggaac agcgtttgcg
      421 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
      481 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
      541 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
      601 cgtacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
      661 cactcgccac cagcacgctg aacacattac cgaagttaga gctcgccgtc attttgatgt
      721 atttcagcat gttggcgaaa gtgcgacgtc cctcaataac cccctcttcc agcaccatca
      781 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
      841 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
      901 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
      961 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     1021 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     1081 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     1141 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     1201 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     1261 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     1321 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     1381 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     1441 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcgatttttt
     1501 gccaacgact ggccagcgag cgcgctgact cttcatccgt accttcgagc accgctgtat
     1561 cgagcaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     1621 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     1681 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     1741 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     1801 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     1861 ccgcttccca ccagtcgcct ttggtgtaac cattgattaa cagcaccacc ggagccatca
     1921 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccttgctga aaggcattcg
     1981 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggta ttggcacctg
     2041 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     2101 tgtcgcactc cagcggattg ctgtgctccg gctggcgagt ggtagctgct ttttctacgg
     2161 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     2221 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     2281 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     2341 tcgcagtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     2401 gaataaagtt cagcaacgta gaaatggcga ccattagcgc gataacgccc gcggcaaata
     2461 aatcttccgt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aaggggttgc
     2521 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     2581 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     2641 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     2701 aggacggcgg gaccaccgta ttcactgtct gctgcgcgcc aggcaatgga tcacgatgaa
     2761 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     2821 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     2881 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtaacgccgt
     2941 gaaaagacgg gcttaccgaa aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     3001 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     3061 attcgtagca tttatggcga tataatacct gctggcaact aaaccaacga taaaccagac
     3121 tttacccatt gctgaatgca cgggtaaagt tagcctcaaa taattaaaca acacgttacg
     3181 ggacaacagg atgcaaaatc ggctgaccat caaagacatc gcgcgcttaa gcggcgtggg
     3241 gaaatctacg gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacgcgcga
     3301 gcgcgtggaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     3361 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgcctggatt cgttgtcaga
     3421 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     3481 gatggaaagt cagttttccc cgcaattagt tgccgaacat ttggggatcc taaaacggcg
     3541 taatatcgac ggcgtagtgc tgttcggttt taccggcata acagaagaaa tgttagccca
     3601 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     3661 tgacgacgaa ggggcaatca agatcctgat gcaacggctc tatgaccagg ggcatcgtaa
     3721 tatcagttat ctcggcgtgc cgcacagtga cgtgacgacc ggtaagcgac gtcacgaagc
     3781 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     3841 tatgaagcaa ggctatgaga acgtagcaaa agtgattacg cctgaaacta ccgccttact
     3901 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     3961 caccttgcaa ctggcgagcg tcggtaatac gccgttaatg aaattccttc atccggagat
     4021 cgtcaccgta gatcctggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     4081 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     4141 gtttcctgaa cgataaattg tgatcttcgc tgcgtttcgg gaacgttccc gtttttaaat
     4201 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     4261 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     4321 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     4381 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     4441 ggcaatttca ggtggtgatt ggcaccaatg tgggtgatta ctatcaagca ctgattgcgt
     4501 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     4561 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgctg ctgcccgcgt
     4621 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     4681 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     4741 tgatcggtga agcgatcttc ttctacctgc cggtcggtat ttgctggtca gcggtgaaaa
     4801 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tctccccagc
     4861 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     4921 gcatcgccaa agtaggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     4981 tgggcgttat tgaaactcgc cttaaacgca tcgtgccgga ttacctctat ctggtggtgg
     5041 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     5101 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     5161 ctccgattgg cgcagcattg tttggcttcc tgtacgcccc gctggtgatc accggtgtac
     5221 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     5281 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     5341 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     5401 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
     5461 cgatgattgg ttctggtctg gcaggattac tatgcggcct gaacggcgtt atggcgaatg
     5521 gtatcggcgt aggcggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
     5581 ttgcgctggc aatggttatc gccatcatca tcccgattgt actcacctcg tttatctatc
     5641 agcggaaata ccgcctgggc acgctggata ttgtttaatt ttcttcgggg cgcaattgcg
     5701 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
     5761 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
     5821 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
     5881 ctaaccccct tttatatctc tccccaggtc gataacggtt acgacgtagc gaactatacg
     5941 gcgattgatc ccatctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
     6001 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
     6061 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
     6121 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
     6181 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
     6241 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
     6301 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
     6361 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
     6421 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
     6481 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
     6541 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
     6601 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
     6661 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
     6721 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
     6781 catggcatgc agggaacgcc gtatatctac caaggcgaag agattggcat gaccaacccg
     6841 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
     6901 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
     6961 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
     7021 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
     7081 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
     7141 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
     7201 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
     7261 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
     7321 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
     7381 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
     7441 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
     7501 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
     7561 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
     7621 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
     7681 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
     7741 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
     7801 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
     7861 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
     7921 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
     7981 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
     8041 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
     8101 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
     8161 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gtcagcacct gctgccgcgt
     8221 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
     8281 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
     8341 aagatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
     8401 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
     8461 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
     8521 gagtggaaca tcccggacgc cgaaggctac gcaaactctc gtaccatcaa agagtgctac
     8581 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
     8641 ccgtttgtaa cctttggttt tggtctgggc accagctggg aatcgcgcct gattcaggaa
     8701 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
     8761 aaactggtgt ttgcgattcg cgatggtctg aaccataaaa aaggcgatcc gaactacgac
     8821 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
     8881 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
     8941 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
     9001 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
     9061 aagctgctgg atgaacgtct ggtgctggca cgtaaggcgc tgatgacccg tatcgctcgt
     9121 ctcgaaggcg tgaaagcgcg cgtggccccg atcctctata tggaaggtgc ttgtggcgta
     9181 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
     9241 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
     9301 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg ccaggcagtg
     9361 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
     9421 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
     9481 gataaaggtt actacaccaa cagtttccac ctcgatgtgg agaagaaggt gaacccgtac
     9541 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
     9601 ggcgagtatc caaacattca gcacaacctg aaggcgctgg aagatgtctg ggattacagc
     9661 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
     9721 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
     9781 gacgcctccc gtgtgtcggt gactcgccgc gtgtgcggat atttaggtag cccggatgca
     9841 cgtccattta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
     9901 gggcagatag gttaatcttc actctacgtt aagcaaatgc cggatgcggc gtaaacgcct
     9961 tatccggcct acatggcaac aacgattgta ggtctgataa gacgcgacag cgtcgcatca
    10021 ggcgttgatt accggatgca tcctatacgc ggactgataa gccaggcgaa acaatgaatt
    10081 atcatcagta ctatcctgtc gacatcgtca acggccccgg cactcgctgc accctgtttg
    10141 tctccgggtg tgttcatgaa tgccccggtt gctataacaa aagcacatgg cgggtaaatt
    10201 ccggtcagac atttactaaa gcaatggaag accagattat taacgatctg aatgacactc
    10261 gtatcaaacg ccagggtatt tccctctccg gcggcgatcc gctgcatccg caaaacgtgc
    10321 cggatattct gaaactggta aaacgcatcc gcgccgagtg tccgggtaaa gacatctggg
    10381 tgtggacagg ctataaactc gacgaactca acgctgcgca aatgcaggtt gttgatttga
    10441 ttaacgtact ggtcgacggc aaatttgtgc aggatttaaa agacccttcc ctgatctggc
    10501 gcggcagcag caatcaggtg gtgcatcatt tgcgatgata agttccctgt tacctgagtg
    10561 caggcaacag ggaaatgagg aattaacgat acttctggtg ataggcgttg cgggtcgttt
    10621 tcagttgctc tgcagcagcc tgcgcttctt ttactttacc ttcatttgcc agcttcagcg
    10681 cgtcgtcaat ctgaccgacc agaatgtcga aaccgtggcg gaaatctttc atttccgggc
    10741 tgtccggtga tttatcttcg agcttcggcg gcgttgcttt ttgcgcatcc agcgctgcgg
    10801 cgcgcatctt cgttaacgcg tctttgactt gcgccgcgtt atccgctttt tcgatcactt
    10861 ttaaattgtc gttgagggtt tccatatt
//
LOCUS       DAZFJF010000139        10879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFJF010000139 DAZFJF010000000
VERSION     DAZFJF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.140"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..339)
                     /locus_tag="ACHKAU_004133"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHKAU_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(655..1299)
                     /locus_tag="ACHKAU_004134"
     CDS             complement(655..1299)
                     /locus_tag="ACHKAU_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533092.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1320..1589)
                     /locus_tag="ACHKAU_004135"
     CDS             complement(1320..1589)
                     /locus_tag="ACHKAU_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5533093.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1668..2306)
                     /locus_tag="ACHKAU_004136"
     CDS             complement(1668..2306)
                     /locus_tag="ACHKAU_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO5533094.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2291..3522)
                     /locus_tag="ACHKAU_004137"
                     /pseudo
     CDS             complement(2291..3522)
                     /locus_tag="ACHKAU_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(4023..6704)
                     /locus_tag="ACHKAU_004138"
     CDS             complement(4023..6704)
                     /locus_tag="ACHKAU_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO5533095.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6945..7991)
                     /locus_tag="ACHKAU_004139"
     CDS             complement(6945..7991)
                     /locus_tag="ACHKAU_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFO5533096.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNASSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(8095..8244)
                     /locus_tag="ACHKAU_004140"
     CDS             complement(8095..8244)
                     /locus_tag="ACHKAU_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533097.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            8666..8857
                     /locus_tag="ACHKAU_004141"
     CDS             8666..8857
                     /locus_tag="ACHKAU_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5533098.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(8910..9143)
                     /locus_tag="ACHKAU_004142"
     CDS             complement(8910..9143)
                     /locus_tag="ACHKAU_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533099.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9239..9862)
                     /locus_tag="ACHKAU_004143"
     CDS             complement(9239..9862)
                     /locus_tag="ACHKAU_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5533100.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(9951..10460)
                     /locus_tag="ACHKAU_004144"
     CDS             complement(9951..10460)
                     /locus_tag="ACHKAU_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533101.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT         3015 a         2762 c         2192 g         2910 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcacagt tcatatcctt
      421 atccataaaa aatttagcta tgtctatagc attaatcttg acaagacgac gttagggcct
      481 ggcgatgaat catttcccct gccagatatc ctttggggtg caccagaact accgttttat
      541 atctatataa ctgaaacgtc cctcgccatc cttcatattt tgatgtagta acaccattca
      601 ctttcatcta ccccccgttt tattaacata tagtaaaaat cacctcgccg cattttattc
      661 ttttgaagta aaatcattta ccagttcact taaagctctc ttttgttcat catcatacag
      721 agattgaatc cggaatttaa tctcttctgc aaccagtctt gcatcgggct cataaaatat
      781 ttttgttatt cgctgtttct gatgttcagg ctggtcttcc atatttcgta aaaccagatg
      841 aaataatata tcataaagtg cgcaatgcca tgaataacca tcattggtta aaataagctc
      901 ccaaacccgc tcctgctcgg caataagctg cagaacaaag gactcaacct gttctgtttg
      961 ttcgggggca acacggtata gcggagcaat cacagcaaaa gatagttcat ttaaccagga
     1021 ttccgcttcc ttctcgctcc tgaacggtgg acagttttgt attcttcgtt cgtattcacg
     1081 ggtaatctgc tccagaatat cctccggcgg tgtcagccac ttattcctga gcaatacact
     1141 ggttgatgga tgacagctgg ctatttcagc aataatttca cgtaatgtct ccgggctgaa
     1201 cttcttcagc tgattgtagt gctcccgggt aaaaccatgt tcatcgcata aaagttgcca
     1261 tgtcattgat atactgtttg ccattccttg cttcatcatt aatacctgtt acctgaatat
     1321 caacagaccg gtgtaccttt tctgaatacc tccccggacg gagtggcaat ccccattcgc
     1381 ctcagcgtgg catctgcagc tttcgccacc tcgctgatat ccagcgtttc aggtagcggc
     1441 ggggacgata tctgcgccag acaataattc cggcgcaaca acaaccactc ccgttgtgct
     1501 tctgtacatt caacatttcc cataacaaaa agccagcggt cagaagctct gcgccagcaa
     1561 ccagcagcct ccagtttctg cgcaaccatg tcttccacaa cttccggggt actctttttc
     1621 ctgctcaaaa cattaactca ctgaaataat atacatatct gacggtctca gcgaaccgtc
     1681 catgccgggc tgaaatcctc ttcctgaaac agctccgtca gcccgctaat ctgtttcaat
     1741 ctgagaactt catcctggtc catacccagc tccgtaccaa tgcgttcatt cgtccagccc
     1801 aggcgcgaca ggtccctgac aatatcagac attgaggtta tctgatgttt gcccctggcc
     1861 cggttatgtc ggatggtcgc agcaatacgt gaggcctgtc cctttctctc cggattaata
     1921 cagaccaccg gcagccagcc cttcagacgt tttcccgtac ctgcttccct gcccagtacc
     1981 tggcgatgaa aaccatccac caccaagtaa tggttctttt cctcagacac taccaccggt
     2041 tgtgtgaaac catctttctc cagtgactgc ttcagcagtt tcttctcacc cggcgccatg
     2101 acattaggat tgtaatcatt ggccaccacc tcatctgctt ttacccacag cacacagtcc
     2161 acaggctcct gtgcaaaagg actgacctca tgaagcaccc tgcgcaggtg attaagggcc
     2221 tgtatggcct cttcttctgg tcgttcctgg ctgaagtact gacgaatcat gttgccaagt
     2281 atctccggtg tcacagaatc ccccattcct tcctcctttc cttcatacgc tgcaggtaac
     2341 gttcatagtg ccggggtttg ttagggctaa aggagagggt ccggcaccag aaatcattct
     2401 ttatgagagt cttacagata cgtcgccagg acggaatatc ccggctcccc aggtcattct
     2461 cctgttcatc cggaatgtca tccgggaagc cccgcgtctg ataccaacgc aggtagacag
     2521 caattttatt acggtaatgt tctgctgttc tttccggcat cacatccagc aggaacatcg
     2581 catagctgcg ccaggtatga tgaggcggct tcgtgatacg cttacacagg gcaaaatagg
     2641 caccactctc attggcataa agcgccccgc tggcagcccc tgacacccgt tcacacatcc
     2701 tggcccaggt ttccggctcc agaacatggt aaagccacag tcccttacgc tgttcaggac
     2761 caaaaggttc acagacacgc atgttgcgta acggcacgcc ggcacggtac atcaggtcat
     2821 acagaggatt gtaaatggca caggttctgg cgttatatat ccagatatcg cgggccttcc
     2881 agtcatacaa cggatacatg gtgtaataaa acccttcagg cgatgccgtg gtccagggct
     2941 tatcatcggc atatctcagt ttgcgctgag ataccagtcc cataaagcga ttgagcgatt
     3001 catcagcacg gacaccagtc agtattgcca ctccacaccg gttaccggca aaccaggaag
     3061 aaaatgccgg aacaaattct tcaaacgtca tggcataccg gtaaaaggga aaatacgtca
     3121 tgtctgtaat ggcctcttcc ggtggctggc gaacccaggt cactcctggc tcccagcata
     3181 tccactccgg ctgaaactgc gagacgccgt ttaccgtggt cagggaagtg ccacccagta
     3241 aaaggtttcc gtcacatcat ggtacatttc ccgcatcttc tgaatatgtt caatagtgca
     3301 ccgatactgg gcctcccagt caatgaacag aacagagaaa tgacgttttc tcctgcgggc
     3361 cacctctgcc acaagatgga aaagcacagt ggaatccttt ccacctgaaa aagacaggca
     3421 taccgacgga aatgtttcaa aaatccactc aatgcgggcg attgcggcac gcaaaacatt
     3481 ttctccggta aggatttttt cggttaaaag cgtatcactc attttcctgg ctttatgtcg
     3541 aataattctg tttatgcatt cagacaaact tcattaataa ctcaccgctt tggggataaa
     3601 taataacaat aaaactcttc agatattaat ctggttgttg gatttgttat cacatccttt
     3661 atccacctgc ttcttactgg taacacatct ggaaaatcca tgcattttca acatatacca
     3721 tcctttcaac ttgtaaataa ggaaatatga tgcagatggt gaacaattca aatatcattt
     3781 tgcaggaagt attcactgta atagaattac cccctgataa agtttattca ctaaggaatt
     3841 attcaacaga accattccct gttagctttg agcaatagca cttattttta tatgtatcag
     3901 gaatacgtac gttgatgtaa ccaaaacaaa gaagcctgtg tccatggggt tcgtacaatt
     3961 gtgtgtggca catgaccaaa tcaggtttga gacctcccgc tgcaatattt tttattcaga
     4021 ctttaacctg tcaggcctga cagaaacgct cccaacttcc cttctatatc actgtctata
     4081 gtacgtaatc ttagcaatgg caggccgcat ttcttcagta tactgttttt aagttcatca
     4141 cgttcagcct gaacactggt cagatgataa cctccatcca cttcaataac ccctaatgga
     4201 gtcttcccga ccttatagta aacaacaaag tcacaactgg cccggttacg catgaataac
     4261 tgttcacggt gtgtaaaatc cccccgctcc agcaacacca actgattcag cgctatttgt
     4321 gaatgaaaca tcatactccg ataactatcc tgcgacagga tatcccgcaa caggcaggcc
     4381 acaatctgtt ctgacttaaa gtgtgaatca tttgaattca atctactatt cagtcgttcc
     4441 agtgatttgt catactcact gtacagcaaa tcaaatgctg atatgaccgg gctctcgaaa
     4501 atctctccgt catcagcgta gtatttgatg taacgaatca gcgccgcaat atgtccagcg
     4561 tggcgttcaa atacatcatt accagtcacc agcgtaaatt tatttcgggc ccttgaaaca
     4621 gcgacattca ccagttcagg attatcaaca aacgcgatgt tccgactgtg ctggctgctg
     4681 cgcttcttat ccagcacagt ggaaaacaca atttcatcgc actcccgtcc ctgaaattta
     4741 tgcaccgtcg atttcacaaa atcagcaggc agcactgttt cagccagatt aacctgtgca
     4801 ttatatgggg cgatatatcc cctgctactt tcctcatccc agtaatgtcc ttccagagat
     4861 tcaagctccc tgagatttga aaaattacgg gtatggtttc cttttgccgt gatgatcaga
     4921 gataatgagg cttcacctga atcaagcgtt agcggtatca gagcattatc ataaaactgc
     4981 ttattacaaa attgaatgat tttaggatga cagcgataat gctctttaag aagagttact
     5041 ggcaccatat tcctgaacaa catacaaacc gaatcaagta gactatattt ctcacaatta
     5101 tagtattctg ccggtggaga tgggctgttc gggagtaata caggaacatg tggaagttgc
     5161 tttcgatccc ctacaacgat tacattacgg gcacatccca gtcccagaat gcccggaaca
     5221 atatcctgct gtgaggcttc atcaataatc acataatcaa gaagagcccc cttaccgata
     5281 gagttaataa tagaatgcgt gctgctacca ataaccggaa aacgctttat aaaacggtta
     5341 aattcatccc tgtatgtctc tgccgagaat gaatttcttg ttgaaatatt tcttcgaaga
     5401 tgttgtttta aataaagcac agaccatgat gtcaggcgct ccagtaaaat tttaaaatca
     5461 gattccttta atacttcctc atattcattc agagaaactt gtttttcccg gatgaatttt
     5521 tcataataag aaagctgaag agaagtaaat agtgccagcc ttctctccgg aatcaccaat
     5581 ggtttgactt tcagtatcct gaagtttaac aacaaatcaa ttctgtttct gaatccaatc
     5641 cgcctgtcag aaaggtaatg aatataagcc atcaggtcag ttgtttttcg tgaggaaagt
     5701 cggtatttgt gagaaaaaag ctcctccggt cgaagattct gactctgcca ttgctgcagg
     5761 tatttatact caatattcag ctcattaatt tctgctttta acgaagcgac ctggttaatg
     5821 gcattcaggt gttttttcac ttgttgaagt acatcatcaa tcgcatttgc agacggtgag
     5881 tcaggtaaga cttcctcaga acgcgaaatc gacgttgaaa aaaactgttg cctgttctcc
     5941 gtactcccca gtcgtgccac cacatatccc agttgctgtt tgtccatctt ttcataaaca
     6001 ttactgacag ctgagttatt attggacaaa attgccactg ttttattctg aattaatata
     6061 ttagcaacaa tattgagtat agtctgtgtt ttacctgttc caggtggtcc ttcaataact
     6121 gaaacctgtg aggaaaatgc ccgttcgaca gccagcaact gactttcatt aagaccaaac
     6181 ggaaaaatca aaccccatgg cgaatcacgt tttttactct gtcctgaaca gtatgcatgc
     6241 aaggcggtat ccggatgagg aactattttc tttatctgcc taaggatatt ctcatcgata
     6301 tttctgttat taccgcgggc ataaaaaata cgttcattag ccaccctaca caagtaatta
     6361 aacacggcat gttctgtaaa tgctgaacac tgttgaaagt caagaccggt cgttttcatg
     6421 atgtagggtc gttcaccctc gcgataataa acagcagcat atttatcacc atatataata
     6481 accagttcag cccgggatgt tacagcgttc cctttccttt cgaggaaaac atttcgcaaa
     6541 tttacagttg gcgttatctg acatacagaa agcggacgag tatattttct cccgctgcga
     6601 aaatgacagg tcagcataac ctctctgtcg tcatttccag cccacagcgt ccagtcggta
     6661 attttatgag tcttattttc accgtcaaca caaatcgtga ccatattctt actcagaaat
     6721 tatcatttaa cttacggaaa attgttaagg cactcccgat attagctccg gaggattttc
     6781 cacaactccg ggactgttaa catatttctg cctgccatat atacattgaa tttagtcata
     6841 acagtcaata ccagaattac aggaatgcga tgccgatacg ctggttattg aatactggta
     6901 gcccctgagc actaattcat cacgatactc tgtatttact gttttcactg ctttttacaa
     6961 ccattccccc aggtaaaatg ctgctcctcc ggtgatggct gtgtcagaaa ctgccatccc
     7021 ggaatagcca cgctgaccgc cagcaatgcc gcacagacac cagccacaaa cggtttcaga
     7081 aacgtgggcg gggatttctg aagggagggt gactgctccg cctgcacttt ccccttgcga
     7141 ttatcaaggc tcagtgcatc cagacgcacc gcgccatttg ccgcaagcag cccttcccgg
     7201 ttatccacgc ttcccggcag actcagcgaa aaatcccctg ttcccgtctg aatcagactg
     7261 cctttacgat ttgacaggct ttgtgccgat atctgcagtg tgtcggcatt tatcgtaccg
     7321 ccattactgc tgaactgccc ggcagtccgg gcggaaagcg tatcggcact cagttgcgca
     7381 ttctgtgtag acagatttcc actgcttgca ttcagctcag tatgttgtcc ctgtatccgg
     7441 ctgttaccta aatccagccc ctgctcaatg tcccgtaaat ccagcagaga accttcgtgt
     7501 gccgggaatg aggaatacaa ctgaatatag tcatcactgt ccggtgtcag acgcacatgt
     7561 cgcacaacac cgggaataat aaccagccgg aggataccgc tttttaaatc ctgcgaaggt
     7621 gccagaacac gggtggtgac ataaccatga tcgaccagac ggttctgcaa tgtactcatc
     7681 agcagattaa ttcctttcgc ccccaggcaa tgcccgaccg ccccgttggc gattttttgt
     7741 aaaggcagcc agtgtggtaa cgcatcagcc cccttcagtt ccgtctgttt aatctgaaaa
     7801 cagggcgttt caacaggaaa attaatctta tgggcaaaag agccaggtgc agaaaggcga
     7861 acatcagggg ctgaaggcgt cagttgttgc tcaagggctt cctgacgctg ctgctgaatg
     7921 ataaatgctt catctccgct acgccggaca tccgctgcct gaagagataa aggcgataat
     7981 aaagcagaca tcagtgtcag tgacagcaag gacggacagc gccggaataa gctttgttcc
     8041 tcagtcctct cgttcctgca acataatttt ccagtcaaac gaaaaacctt ttattcatgt
     8101 cataccaaca atcaccagcc tcctgacgat acctcccacc atgttttgcg atggcacgct
     8161 accgcccttc cggtatttac ccgcatcaac cggtaccaga atgctgagta acaaaccagc
     8221 tagaattgac ttaattttga tcattataga ataatgatct gaactacagc aacatgacag
     8281 atttatcgat cgatataaca caaatgaaca gttctttatt ctgacaaata tcgcaaaaac
     8341 agcacattac aaaatttata accataatta ctggaaaagt gcagaaaatt attccactga
     8401 atgcttttaa tacagttggt tagcttaaaa aatagcaatt tttgatttca gtcattgaaa
     8461 gttgcaatgt ttgacgattc aacagcattt aagtgagttt caggatatgg gaaggacatg
     8521 aaaggaggag cgcaataaaa tacacaggaa gaaggggaaa atgtttcgta aaaacagaaa
     8581 attaacagcg cacatcagga aacgcagtct cctgattcag tgctaccaca tgaaaattta
     8641 gttaactgtt accgtactaa aatttatgtc agaagatgtt ccattaccga aagtcaatca
     8701 gcgatacaaa gacgatcatg gtgcgcttgt caccctgacc agtgttgagg aaacccgggt
     8761 ggtgtttatg caggacggct atccgcatcc ctgcatgcga ccgatgtaca actttctggg
     8821 aaaattcaaa cccgaacccc gggaagaaac agaataaaac gaaatccctg ccggcaatca
     8881 cactacctga tagcagcata atccacttat taattctcat ccccggagac aagcccagaa
     8941 aaccactccg ggtgttcact gtatgccgtg ttcagcgcat ccctggaaac gtttatataa
     9001 aagctgtcct gcaatttgcg ggtaacaact tctgccaggc gattccggtt atccaccagt
     9061 ccggcataac ctctctctgc cagccactca ttcagttcat gttccagaga cagattgata
     9121 atatcataat cggaccttgc cattcccgtt tccttagcac atgttttcag gcacggggat
     9181 aaagtataaa gaatgtcata ctgctgctgc cagagcaata ttccccctga tggttgtatc
     9241 agagatggta tgccggtact ttgcgggttg ttcccgtcag gttatcgtgc acctccacgg
     9301 tcgtggtcac caccggcatt ccggcctttc tcagcctcag aacatcagcc gcaatacgct
     9361 gactgccaaa ccagaacagg ccgtccagtg cagtcaccag taaacccgcc tccagtgcat
     9421 acttcagccg ttcacggggc gctttcactt cccgggcaac ctgctgatat ggcaatgatg
     9481 tgttttcatt cccaaccacg cgacgaatac ggtgagacag atgataccgg tatgtatccg
     9541 gcacaccaga aaggctggcc ttcaggctgt acacgcagcc aaaccgttta tcgttaaaca
     9601 ccacattttt ctggctgatg ccccattctt cacgcagcgc ggcaatcaat tgcggtgtac
     9661 gggtaagcag caagcgaaaa ggcagttcaa aactggtgac gtaatccaca ttcagcagtg
     9721 caatacgcaa ccgttcttct ggcccggcct ctgtctgccg gcactcctcc agaacatcct
     9781 gccactgccg gcgaagacgg ggggattcgt tccgttctgt aaaacagtat ttatccgcca
     9841 gatagtcaat tcgtgtacgc atactgaaag gaattccgta taaagattca gcagacaaaa
     9901 ctttatcagc cagtaaaaat taacggaagc attgatattc ctcccgacaa tcaccggatg
     9961 attgttgcaa taacctgcga cataaaggac tgaacagccg tttttaacgc cacgtattgc
    10021 cctgatgaac caggccgggc cacccgaaaa catctttcca cccggcattg tccgccagca
    10081 cttatcaccg accaggtctg ttgcagtaaa tccaccagtt gcgcacattc ttcatcagcc
    10141 atgacgccga ctcgcggatg gcaaatactc gtctggctga gcagcaacag acatcgtatc
    10201 tgtgctcctt catgttgttc ccgcaaccag cgtaacacca ccggcgctga tgggctgacc
    10261 gtgtgattat gctccgtaat acgttcgtgt acccgtcggt gaaaggaact acaaaatgtc
    10321 tgaatctgtt gaccatagat tccttctgcc gaatagagcg tgaagtgaaa tgccaacaga
    10381 tttggccagt ggtccaccgc ctgctgaaca aaacgccgga tttcccccgg ctctgaaatt
    10441 aaggcttcgg ttatttgcac gaaatgttcc ttttcaaccc ctgttgaatt ttgtcaagcc
    10501 ggtgcagaca cagtaaagcg cgcatcaaca cctgaaaggt acgatacaaa cacaagttac
    10561 aaacaataag aacggaagga caattaatca gtgaggaata ataactaaat taaccaaaag
    10621 aatgttactg acaatactac caatacttaa atagcaatat attaattaat gtaagaacag
    10681 atgcccggta ggacatatac acaatacatc cacaaacaca ctcttttcat taaatcaatc
    10741 acctgattaa accatgaaag tgtgatgtaa gtcaaaatta ctgatactat caattgataa
    10801 tgattatctc tatcattatc gtgaaattcg agaatgaaag gagaagtagt cattataagc
    10861 cttttataaa tcgtcatca
//
LOCUS       DAZFJF010000140        10770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFJF010000140 DAZFJF010000000
VERSION     DAZFJF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.141"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            101..421
                     /locus_tag="ACHKAU_004145"
     CDS             101..421
                     /locus_tag="ACHKAU_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFO5533102.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            436..2769
                     /locus_tag="ACHKAU_004146"
     CDS             436..2769
                     /locus_tag="ACHKAU_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO5533103.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRHLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            3353..3481
                     /gene="rhuM"
                     /locus_tag="ACHKAU_004147"
                     /pseudo
     CDS             3353..3481
                     /gene="rhuM"
                     /locus_tag="ACHKAU_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            3910..5094
                     /gene="setC"
                     /locus_tag="ACHKAU_004148"
     CDS             3910..5094
                     /gene="setC"
                     /locus_tag="ACHKAU_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO5533104.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            5205..6128
                     /gene="yicL"
                     /locus_tag="ACHKAU_004149"
     CDS             5205..6128
                     /gene="yicL"
                     /locus_tag="ACHKAU_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO5533105.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6132..6950)
                     /gene="nlpA"
                     /locus_tag="ACHKAU_004150"
     CDS             complement(6132..6950)
                     /gene="nlpA"
                     /locus_tag="ACHKAU_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO5533106.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            7172..7465
                     /gene="yicS"
                     /locus_tag="ACHKAU_004151"
     CDS             7172..7465
                     /gene="yicS"
                     /locus_tag="ACHKAU_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO5533107.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(7506..8696)
                     /gene="nepI"
                     /locus_tag="ACHKAU_004152"
     CDS             complement(7506..8696)
                     /gene="nepI"
                     /locus_tag="ACHKAU_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO5533108.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(8907..9359)
                     /gene="yicN"
                     /locus_tag="ACHKAU_004153"
     CDS             complement(8907..9359)
                     /gene="yicN"
                     /locus_tag="ACHKAU_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO5533109.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(9412..10746)
                     /gene="adeQ"
                     /locus_tag="ACHKAU_004154"
                     /gene_synonym="yicO"
     CDS             complement(9412..10746)
                     /gene="adeQ"
                     /locus_tag="ACHKAU_004154"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO5533110.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
BASE COUNT         2789 a         2824 c         2585 g         2572 t
ORIGIN      
        1 ttcgcgcagg gatacgcacg tcttcaggag acgcagatat gccttatcag ttaatgcaac
       61 cggcacggaa tgcagtcatc tgtcacaggg aggaaagcaa atgaaaacac ccttaccgcc
      121 cgtcttacgc gctgcccttt accgtcgcgc tgtcgcctgt gcctggctga ccgtgtgcga
      181 acgtcagcac cgctacccgc atctcaccct tgagtcactg gaggcggcca tcgccgctga
      241 gctggaaggc ttttatctgc gccagcacgg tgaggaaaaa gggcgtcaga tagcctgtgc
      301 cctgctggaa gatttaatgg aatccggccc cctgaaggcc gcgccgtcgc tgtcctttct
      361 cgggctggtt gtgatggatg aactctgtgc ccgtcacata aaagcgccgg tactgcactg
      421 aaggagaaca acaccatgaa aatgaacgta accgccaccg tcagccatgc gctcggccac
      481 tggccgcgta ttctcccggc gctggggatt caggtgctga agaaccatca tcagccctgt
      541 ccggtctgtg gcgggagtga ccgcttccgt tttgatgaca gggaggggcg cggcacctgg
      601 tactgcaatc agtgtggtgc cggtgacggc ctgaaactgg ttgaaaaggt gtttggtgtt
      661 tccccgtccg acgcggcagc aaaggtggct gccgtgaccg gcagcctgcc cccggctgac
      721 ccggcagtga cggccgccgc cgttgctgaa acagacgctg cccggaagaa cgccgccgca
      781 ctggcacaaa ccctgatggc gaaaacccgt cccggaaccg gtaacgccta cctgacccgc
      841 aagggctttc ccggccggga atgccggatg ctgaccggca cacacagagc cggtggcgtg
      901 agctggcgcg ccggtgacct tgtggtgcca ctgtatgacg acagcggcga actggttaac
      961 cttcagttaa tcagtgctga cggccgtaag cgcaccctga aaggcggaca ggtcaggggc
     1021 acctgtcaca cccttgaagg acagaatcag gccggaaaac gtctgtggat agcggaggga
     1081 tacgcgaccg cacttaccgt gcatcacctg accggtgaaa cggtaatggt ggcgctttct
     1141 tccgtgaacc tcctttctct ggccagcctt gcccggcaga agcatccggc ctgtcagatt
     1201 gtccttgccg cagaccgtga cctcagcggt gacggccaga aaaaagccgc cgcagccgca
     1261 gatgcgtgtg aaggtgttgt tgccctgccg ccggtcttcg gtgactggaa tgatgccttc
     1321 acgcagtacg gcggggaagc cacccgtaag gccatttatg atgccatccg gccaccggct
     1381 gaaagcccgt tcgacaccat gagcgaagcg gagttctccg ccatgagtac cagcgaaaag
     1441 gccatgcgta tctatgagca ttacggcgag gcgctcgcgg ttgatgccaa cggccagctt
     1501 ctgtcccgtt atgaaaatgg tgtctggaag gtgctgccac cacaggactt tgcccgggat
     1561 gtggccgggc tgtttcagcg gctgcgcgcg ccgttctcct ccgggaaggt ggcctccgtg
     1621 gttgacaccc tgaagctgat tattccgcag caggaagccc catcccgcca cctgattggc
     1681 tttcgtaacg gcgtgctcga cacgcagaac ggcacgttcc acccgcacag tccgtcacac
     1741 tggatgcgca ccctgtgcga tgtggatttc accccgccgg tggacggtga aacgctggaa
     1801 acccacgctc ccgcgttctg gcgctggctt gaccgtgccg ccggtggccg tgcggaaaaa
     1861 cgcgacgtga ttctggctgc actgtttatg gtgctggcaa accgctacga ctggcagctc
     1921 tttctggagg tgaccggtcc cggcggcagc ggcaaaagta tcatggccga aatagccacc
     1981 ctgctggccg gggaggataa cgccacgtcg gccaccatcg agacgctgga atccccgcgt
     2041 gaacgtgccg cgttaactgg cttctcactg atacgcctgc cggatcagga aaaatggagc
     2101 ggcgacggtg ccggactcaa ggccatcacc ggcggcgatg cggtgtccgt ggacccgaaa
     2161 taccgggatg cgtactccac gcatattccg gcggtaattc tggccgtgaa caataacccg
     2221 atgcgcttca ccgaccgcag cggcggcgtg tcacgcaggc gggtgattat tcacttcccg
     2281 gaacagatag ccccgcagga gcgcgacccg cagcttaagg acaaaatcac ccgcgagctg
     2341 gcggtcatcg tgcgtcacct gatgcagaag ttcagcgacc cgatgctcgc ccggtcactg
     2401 cttcagtccc agcagaactc agacgaggca ttgaacatca aacgggatgc cgacccgacg
     2461 tttgatttta tcggctatct ggaaaccctg ccgcagacca gcggcatgta tatggggaac
     2521 gccagtatca tcccgcgtaa ttaccgtaaa tacctctatc acgcctatct ggcctacatg
     2581 gaggcaaacg gctaccggaa tgtactcagt ctgaaaatgt tcgggctggg gctgccggtg
     2641 atgctgaagg aatacggact gaattacgag aagcgccata ccaaacaggg gatacagacc
     2701 aacctgacgc tgaaagagga aagctacggc gactggctgc caaaatgtga cgaccctgca
     2761 acaacctgac cctcatgact catctgaccg gcatctgccg gtcttttttc atccctgaat
     2821 cccccgaagg tgaacaatcc actgttcacc cttcaccgta tattcacccg ttatcacact
     2881 gaaattaaaa gagaaaaacg aaaggtgaac agtgtgaaca atcaaatcaa aaaaaacttt
     2941 tttcttcctg agtgatttca gtgcggagga ttaatcaccg gtatgagtca caccggcaga
     3001 atgccggagg tgaagaatcg aatgttcacc cttcacccat tattcaccac ctatcaaact
     3061 gaaataaaag gagaaaacag aaaggtgaac agtgtgaaca gttcttccga aaaaaaattt
     3121 ttttcctgag ttaacgtgcg tgaagatacg tgtaacaatt aatcgataga tattatatgt
     3181 ataaggatgt gtataaatga attagaatag aaaaatataa acgatttgaa tcaagtggtt
     3241 atatggcaat tttgactcct gtgatcttcc gccaaaatgc ctcttctgac gttctccaac
     3301 cccttgtaat actgtcaatc cagatatttt ctactgctca atattcaatc ggcttcagga
     3361 atacctggtc taaggttttg tgatggacga tgagcggctg aagaatccgc ctgtcggttc
     3421 ttctgtcctc gaacgcatcc gcgatatgcg cgccagcatg tgccgtgttt atttgcaggt
     3481 gatttgcccc ctgctggctc tcagccaact gtatcgccat taaggtgaag atgttgtttt
     3541 actcgcccca gaacaccctt ttttatacga ctattctatt tctttaaccg ctggcgaaaa
     3601 aaactgacta taaacttcac caataatcag actgccatca agattaattt gttcatttaa
     3661 tatatcaaat caacttgttt tatttttact atcaaaaata gctcattgcc ttactataat
     3721 taatatataa attcggtaat taattcttaa catgcttttg ctaataatct caattgctgg
     3781 ccctataata ttgcgctagc attgctttct ggttgtatca gcgatactca aaaattctta
     3841 atacaatact cattcgactg gtacttattt gtaactcagt tatattttgt cgcccggtga
     3901 ttcagaagaa tgcaaaaaac ggctaccact ccatcaaaaa tacttgatct cactgccgcg
     3961 gcatttttac ttgtcgcctt tctgacgggt attgcgggcg ctcttcagac tcctacccta
     4021 agtatattcc tcggagatga actgaaagcc cgtcctataa tggtagattt tttcttcacc
     4081 ggtagcgcta ttatgggaat tctggtcagt caatttctgg caaggcactc cgataaacaa
     4141 ggcgaccgta aattactgat tctgctatgt tgcttatttg gagtgctggc ctgcacgctt
     4201 tttgcgtgga atcgcaacta cttcattctc ctctcaacgg gcgtacttct gagtagtttt
     4261 gcttccaccg caaacccgca aatgttcgcc ctcgcccgtg aacacgccga cagaacaggc
     4321 cgtgagacgg tcatgttcag tacattttta cgtgctcaga tctcgcttgc ctgggttatc
     4381 gggccaccgc tcgcttatga actggcaatg ggatttagtt ttaaagtgat gtatctcacc
     4441 gctgccatcg catttgttgt ttgcggactg atagtctggt tgtttttgcc atcaatacaa
     4501 agaaatattc ctgtcgttac ccaacccgta gaaattttac cctccaccca caggaagcgg
     4561 gatacgcggc tactttttgt ggtctgttca atgatgtggg cggcgaataa tctctacatg
     4621 ataaatatgc cgctatttat tattgatgaa ctgcatctaa ccgataaact ggctggagaa
     4681 atgatttgta tcgctgccgg tctggaaatt ccgatgatgt taatcgcagg ctattacatg
     4741 aaacgtattg gcaagcgact attaatgctc attgctatcg tgagtggaat gtgtttttac
     4801 gccagcgtac tcatggcgac gactccggcg gttgagctgg aattgcaaat tcttaatgcc
     4861 atcttccttg gtattctctg tggtatcggc atgctttatt ttcaggactt gatgcctgaa
     4921 aaaataggct ctgcgacaac gttatatgca aatacttcac gcgtcggctg gattatcgcc
     4981 ggctctgttg acggaattat ggttgaaatc tggagctacc atgcgttgtt ctggctggcg
     5041 atagggatgt tgggtattgc gatgatttgc ctgctgttta ttaaagatat ttagctcacc
     5101 agtctgggct aagaaaaatc ggcgtggcgc aaacagagcc atcctcgcca tttttgctat
     5161 catgcctgca tacataaacg acaaaacagt atgcagaggg aaaaatgggt tccaccagaa
     5221 aggggatgct gaacgttctg attgccgccg tgttgtgggg aagttcaggg gtctgtgcgc
     5281 aatacatcat ggagcaaagc cagatgtcgt cgcagttttt gactatgacg cgtttgatat
     5341 tcgccggttt gattctactg acgctgtcat ttgttcatgg cgataaaatc ttttctatta
     5401 ttaacaatca taaagatgcc attagcctgc tgattttttc cgtggttggc gcgctaactg
     5461 tacagctcac ttttttgcta accatcgaaa aatcgaacgc agccacggca acggtgctgc
     5521 aattcctctc accgacgatt atcgtcgcct ggttctcact ggtgcgtaaa tcgcgcccgg
     5581 gcattctggt tttctgcgct attttgacat cgctggtcgg gactttttta ttggtgacac
     5641 acggtaatcc gacgtcatta tcgatctctc ctgccgcgtt gttctggggc attgcctcgg
     5701 catttgctgc tgcattctat accacctatc cctcaacact aattgcccgc tatggcacgt
     5761 taccagtcgt cggctggagt atgctgattg gcggtctgat tctgttgcct ttttatgcca
     5821 gacaaggaac aaactttgtc gttaacggca gtttgattct ggcgtttttt tatttgatcg
     5881 tcattggtac gtccctgaca tttagtctgt acctgaaagg agcacaatta attggcggtc
     5941 caaaagccag cattttgagc tgtgcagaac cattaagtag cgcgctactc tctttgctgt
     6001 tgctggggat cacgttcaca ttaccggact ggctgggaac gctgctaatt ctgtcatcgg
     6061 taattttgat ttcaatggat tcccgtcgcc gcgccagaaa aataaatcgt ccggcgcggc
     6121 atgagtgaga attaccagcc aggcaccgcg ccaccgttaa aaatggtttc tgccgcttta
     6181 gcgacttcgg gtgattgata agattgcaga aattccttca cgttttctgc attcttatta
     6241 tcttcccgtg ccaccaaaat attcacatac ggcgaattct tatcttcaat aaatacgctg
     6301 tcgtgcaccg gagaaagccc tgtctgctga atgtaagtgg tgctgataat cgctacatca
     6361 actttgggat catccagtac gcgcgggagc tgcgccccct cgagttccat aatctgcaaa
     6421 tgacgaggat tatcggttat atccagcgcg gtaggtaata acccttttcc ctctttcaga
     6481 gtaattagct tctctttttg caacagtaaa agtgcgcgac caaggttggt cgggtcattg
     6541 gggatcgcca ctgtcgcgcc ttcttttatt tgcgccacgg ttttgatttt tttggaataa
     6601 cccgccatag ggaagacaaa cgtatttccc accgctacca gtttataacc atgcgcctga
     6661 ttatcttgtt caagaaaggg tcgatgctgg aagacattcg catcaagttc gccatgattt
     6721 gtcgcatcgt tcggtagcaa cgaaccgcta aatcctacca gttcaacatc gaggccatat
     6781 ttctcttttg ccaccttttt agcgacttcc gcgacatctt gttctgcgcc atttataacg
     6841 ccaactttaa tgtgttttgc atcgctgcta ctctggtcgc aacctgccag cagaattccg
     6901 gccagcaata atgcggcccc tgtccgtaga tgatgtgttg tcagtttcat ttttttatcc
     6961 ttttattaat gattacctga ctatagcggt aaggtcgctg cggtttaaaa aacgaaacgc
     7021 tatcgataag aataaaaagg aataaaagtg gaatataaag tctggcactt cttactgacc
     7081 acgcaagcgc gtttcgtaca gcatgatgag agcgatgaaa gcaaactaca cttatgcttc
     7141 attcgataca catttgtaaa aggttgaatc catgaaacca acgacactac tgcttatttt
     7201 cacttttttc gctatgccag gcatcgtata cgccgagtcg ccttttagct cactgcagtc
     7261 cgcaaaagag aaaaccacgg tattacaaga tttacgtaaa atttgcacgc cacaggcgtc
     7321 attatcagat gaagcgtggg aaaagttaat gttgtcagat gaaaacaata aacaacatat
     7381 acacgaggcc atcgtggcga tggaacgtaa taatcagagc aattattggg aagcgttggg
     7441 taaggtagaa tgcccggata tgtaagaaca gcaacaacta ctgaccaacg cttctttacc
     7501 tgatatcagg atttcttcat tttcaccttt gcagtaacca acaatgcagt cagcaacatc
     7561 aatgtgccgg acaacatcaa cggcgaagtc agaccaatat tatccagcgc ataaccgccg
     7621 attgccgcgc cacaggtatt agcaagctga ataaccgcca cctgaataga cccggctttt
     7681 tctgcctgat cggccagcga gcgggtgatc cacgttgacc agccgacggg aaccaatgca
     7741 aaagttagcc cccagataat cgccacgccg gtagcaacga ttttatcgct tccccacaac
     7801 gtcagcacca acgcactcac agccagtatt aacggcgcgc ctgctaaggc cagttttacc
     7861 gaacgtttaa gaatgaatga cgaaagcgac gtaccgataa agctggcaat gccaaaactc
     7921 aacagcacca gcgttaagcc atccacgctg aatcccgcca ggttcatata caccgggcga
     7981 atatatgtga agaaagcaaa ctgcccggcg aaagacatga agatggcgat catccctgcc
     8041 atcacacccg gacgttgtaa taagcggaaa gtattttgtt tctgatgcga gggttcgcct
     8101 ggcagtgaag gcaatgattt gataatccag aaaatacaca gcacgcccat caccgccgcc
     8161 gcattaaaga cattgcgcca accgataagc tcgcctaaaa aactgcccaa cggcgcggca
     8221 atcaccagcg caatagaaac cgcgccgaag atcaccgaca gcgccttcgg caccgtacgc
     8281 ggcggcacca gacgcatggt cagcgacgcc gacatcgccc agaacccgcc cagcgccagc
     8341 cccagacagg cacgaccgat taaaagcaaa ctgaatgagt tagcaaagga aaccagcaag
     8401 caggagagcg tcagcaaaac ggcaaacaaa ataacaacgt agcggcggtc agtagcctga
     8461 attgtctggg taataaacaa actggcaaac attgccacaa aggcggtcac ggtcaccgat
     8521 tgcccggcaa ccccttccga aatgcctaaa tcctgggcca ttggcgtcaa caaactgacg
     8581 ggcaaaaact caacgataat cagacaggcg acacaaaacg ccaccgagaa aacggctgac
     8641 cagttcggtc gggtgatggc atccgcgccg cggttttcgg caataaattc actcatggtg
     8701 ttacccgtgg catgtttcag gggaaaagcc gaccacttta acattgaaat gtgtgacgca
     8761 tttaacgttt ttgcaacttt tacggtgttg cttgagctgg agttaagatc gaaacggaac
     8821 agggcaaaga ttaccggatg cggcgtgaac gtcttatcca cccgacaggt caggcgcggt
     8881 ctgtaggctg ataagacgaa acggtttcaa tgcaaccagt gaacgccctc ggcaggctga
     8941 aagaacgcgt tatagcgcat ctgaaaagca ttaatatcct gcatttcagg ttcggtttcg
     9001 cgcagaaaac ctagcgccag cctgacctga gcgtcggtaa tcggcgcggc gaggcgagct
     9061 ttttgtaaaa tccgatgcca ttgcagcagg ttctgttcgc taccatcgac aatgacaatt
     9121 tgccagatga gcaacacctg cgcggcgttt tgcaggtgcg actcatccgg acgatgcaaa
     9181 taacgtaaaa attcgtcacc agctgacttt cccatttgat cgaccatcga ctccacgggt
     9241 acgacatcga tgttcagccg atcgctgaga cggcgaatcg cttttctggc cccggatgtc
     9301 agcacccgaa aaccaaccac aaacactacc gccagcgtgg cgagcattat ccagatcatg
     9361 agttctcctg cgtaatcaga actcatgata tctggaaacc ctcgccagtt actaatccac
     9421 cagaataatc ttcagtgcaa acagagctgc gaccaccacc acacacaggt tcagatcgcg
     9481 ccagcgcccg gtgcatactt tcatgatgca gtacgacata aagccgagtg caatcccttc
     9541 ggtgatcgag aaagtaaagg gcatcatcac cgtggtaata aacgcaggca ccgattcggt
     9601 aaaatcatcc cagttaacgc gcgccaggct cgaagtcatc agcacgccaa caaagattaa
     9661 cgctccggcg gttgcgtaag gaggaaccat cgccaccagc ggtgagaaga acataactaa
     9721 caggaacata acgccaacca caaccgcagt cagccccgtg cgaccaccga ctgccacacc
     9781 agaagtactt tcaatatagg cagtaacaga cgaggtgccg ataaacgccc ccgccaccga
     9841 actgacgcta tcaacataca gcgccttatt catattgggg aatttaccgt tactatctat
     9901 taagcccgct ttatcagtta caccaattaa tgttcctgat gaatcaaata ggttgatcag
     9961 cataaaggag aaaatgatac cggcgagttc aagtgttaac gcgccgctca aatctacttc
    10021 accaatgacg ccgctaatat caggcggaat ggaatagacg ccgctaaaat gaacatcacc
    10081 gaaaaataat ccacagcaag acgtcaccac aatagaaacc agcaccgcgg catgaaaatg
    10141 acgtgatgac aacacggtta taataaaaaa ccctaaaata cctaacaaca cgccgtgaga
    10201 acttaaatcg ccaatcatca ccagcgtgtc tttattggcg acaataacgc cagtattttt
    10261 taatcccatt aaggcgataa ataatccaat tccgctggtg ataccaatac gtaaacttaa
    10321 gggaatgttg gagatcatcc agtaccggat acgaaacagc gtgagcaaaa atagtccaac
    10381 tgcgccccag aatattgcgc ccatcccggt ctgccaggag atgcccatcg cccccacgac
    10441 cacgaaggca aagaaggcgt tcagccccat tgccggagcc agcgccacgg gtaagttagc
    10501 aaatatcccc atcgcaatac tgccgatacc ggcaatcaaa caggtggtaa caaacaccac
    10561 tttcgggtcc atttgtgccg cgccgaggat ttgcgggttc acaaaaacga tgtacaccat
    10621 ggttaaaaaa gtggtcatcc ccgcaatcaa ttctgtgcgg acggtggtcc catgctgagg
    10681 tagtttaaat aatcgcgaaa gcgtccctga ttcattactc acgtaatcgg tattgtcatt
    10741 attcattttt ttgtccattg gaggagattt
//
LOCUS       DAZFJF010000141        10727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFJF010000141 DAZFJF010000000
VERSION     DAZFJF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.142"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(47..400)
                     /locus_tag="ACHKAU_004155"
     CDS             complement(47..400)
                     /locus_tag="ACHKAU_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO5533111.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHKAU_004156"
     CDS             complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHKAU_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO5533112.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2228..2521)
                     /locus_tag="ACHKAU_004157"
     CDS             complement(2228..2521)
                     /locus_tag="ACHKAU_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO5533113.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2796..4052
                     /gene="yjeH"
                     /locus_tag="ACHKAU_004158"
     CDS             2796..4052
                     /gene="yjeH"
                     /locus_tag="ACHKAU_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO5533114.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(4068..4544)
                     /locus_tag="ACHKAU_004159"
     CDS             complement(4068..4544)
                     /locus_tag="ACHKAU_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO5533115.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            4881..6317
                     /gene="aspA"
                     /locus_tag="ACHKAU_004160"
     CDS             4881..6317
                     /gene="aspA"
                     /locus_tag="ACHKAU_004160"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO5533116.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6435..7736
                     /gene="dcuA"
                     /locus_tag="ACHKAU_004161"
     CDS             6435..7736
                     /gene="dcuA"
                     /locus_tag="ACHKAU_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO5533117.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7852..8190
                     /gene="cutA"
                     /locus_tag="ACHKAU_004162"
     CDS             7852..8190
                     /gene="cutA"
                     /locus_tag="ACHKAU_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO5533118.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8166..9863
                     /gene="dsbD"
                     /locus_tag="ACHKAU_004163"
     CDS             8166..9863
                     /gene="dsbD"
                     /locus_tag="ACHKAU_004163"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO5533119.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9900..10475
                     /locus_tag="ACHKAU_004164"
     CDS             9900..10475
                     /locus_tag="ACHKAU_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5533120.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            10582..10657
                     /locus_tag="ACHKAU_004165"
     tRNA            10582..10657
                     /locus_tag="ACHKAU_004165"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10615..10617,aa:Phe,seq:gaa)
BASE COUNT         2495 a         2819 c         2679 g         2734 t
ORIGIN      
        1 agcgcatcag gcaatgatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact
       61 tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg
      121 acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat
      181 cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc
      241 tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa
      301 tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta
      361 gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg
      421 agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga
      481 aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta
      541 catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc
      601 atcatttttc ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga
      661 agccgcgtac tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat
      721 catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac
      781 ggtgttagca acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc
      841 ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag
      901 gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc
      961 ttctaccgca gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat
     1021 ttcaacttcg gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac
     1081 gcgttcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat
     1141 ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt
     1201 gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag
     1261 ctccataccg atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag
     1321 catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc
     1381 acgcatggtg ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat
     1441 cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt
     1501 agagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc
     1561 cggcttgttg atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac
     1621 gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac
     1681 tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt
     1741 accaacctga gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc
     1801 aactgcagcg gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc
     1861 agcaacagct ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt
     1921 accgtcgcct gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat
     1981 attttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat
     2041 ggtcggtgca ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac
     2101 tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc
     2161 gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt
     2221 gcgaggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt
     2281 cattgtcgat cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa
     2341 ctttcacatc cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca
     2401 gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag
     2461 atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca
     2521 ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg
     2581 tttcccggct ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag
     2641 aaaaaaattc tgatcagcac aaaatcgggt gaaaaccctg attcacctca catttcatcg
     2701 caatttcttc atcgccgtca taagcgaatc tgattgtgct accatcgaaa atctacgcag
     2761 ttgcccaaaa tttgggcgca atcggatatc aacccatgag tggactcaaa caagaactgg
     2821 ggctggccca aggcattggc ctgctatcga cgtcattatt aggcactggc gtgtttgccg
     2881 ttcctgcgtt agctgcgctg gtagcgggca ataacagcct gtgggcgtgg cccgttttga
     2941 ttatcttagt gttcccgatt gcgattgtgt ttgcgattct gggtcgccac tatcccagcg
     3001 caggcggtgt cgcgcacttc gtcggtatgg cgtttggttc gcgacttgag cgagtcaccg
     3061 gctggttgtt tttatcggtc attcccgtgg gtttgcctgc cgcgctacaa attgccgccg
     3121 ggttcggcca ggcaatgttt ggctggcata gctggcaact gttgttggca gaactcggta
     3181 cgctggcgct ggtgtggtat atcggtactc gcggtgccag ttccagtgct aatctacaaa
     3241 ccgttattgc cggacttatc gtcgcgctga ttgtcgctat ctggtgggcg ggcgatatca
     3301 aacctgcgaa tatccccttc ccggcacctg gtaatatcga acttaccggg ttatttgctg
     3361 cgttatcagt gatgttctgg tgttttgtcg gtctggaagc atttgcccat cttgcctcgg
     3421 aatttaaaaa tccagagcgt gattttcctc gcgcattgat gattggtctg ctgctggcag
     3481 gattagtcta ctggggctgt acggtagtcg tcttacactt cgacgcctat ggtgaaaaaa
     3541 tggcggcggc agcatcgctt ccaaaaattg tagtgcagtt gttcggtgta ggagcgttat
     3601 ggattgcctg cgtgattggc tatctggcct gctttgccag tctcaacatt tatatacaga
     3661 gcttcgcccg cctggtctgg tcgcaggcgc aacataatcc tgaccactac ctggcacgcc
     3721 tctcttctcg ccatatcccg aataatgccc tcaatgcggt gctcggctgc tgtgtggtga
     3781 gcacgttggt gattcatgct ttagagatca atctggacgc tcttattatt tatgccaatg
     3841 gcatctttat tatgatttat ctgttatgca tgctggcagg ctgtaaatta ttgcaaggac
     3901 gttatcgact actggcggtg gttggcgggc tgttatgcgt tctgttactg gcaatggtcg
     3961 gctggaaaag tctctatgcg ctgatcatgc tggcggggtt atggctgttg ctgccaaaac
     4021 gaaaaacgcc ggaaaatggc ataaccacat aatccggcgt ttcgacatta atcctggcga
     4081 tcgtctttat gatcaaggcg gtcgcgctca tcatcctttc gctggtactc accatcaaaa
     4141 gtattaccgc caccggtccc ggcgctaaaa ccgccgcctg gcatacgaga aaagcgcaaa
     4201 tgcggcatca acttcactgt cagatgcttt tgcaccggcg gtaataaaag cagaagaccg
     4261 aggaagtcgg tgaaaaagcc cggtaataaa agcagcaaac cggcaatgat cagcgaaaca
     4321 cttttaatca tctccgccgc tgggttttca cccgccgcca ttttttgctg catcagcacg
     4381 aaattcttaa agccctggtt acgtaccagt gacatcccga taactgacgt gaatataacc
     4441 agcacgaggg tcagcaatac ccccaataca tgggcaacct gaataaagat tgaaatctca
     4501 atatagacat aaaggaaaat ggcaataaaa ggtaaccagc gcaaaggttt ctcctgtaat
     4561 agcagccggt aaaacccggc tacctgaatg ggttgcgaat cgcgttcagc ttatattgtg
     4621 gtcattagca aaatttcaag atgtttgcgc aactattttt ggtagtaatc ccaaagcggt
     4681 gatctatttc acaaattaat aattaagggg taaaaaccga cacttaaagt gatccagatt
     4741 acggtagaaa tcctcaagca gcatatgatc tcgggtattc ggtcgatgca ggggataatc
     4801 gtcggtcgaa aaacattcga aaccacatat attctgtgtg tttaaagcaa atcattggca
     4861 gcttgaaaaa gaaggttcac atgtcaaaca acattcgtat cgaagaagat ctgttgggta
     4921 ccagggaagt tccagctgat gcctactatg gtgttcacac tctgagagcg attgaaaact
     4981 tctatatcag caacaacaaa atcagtgata ttcctgaatt tgttcgcggt atggtaatgg
     5041 ttaaaaaagc cgcagctatg gcaaacaaag agctgcaaac cattcctaaa agtgtagcga
     5101 atgccatcat tgccgcatgt gatgaagtcc tgaacaacgg aaaatgcatg gatcagttcc
     5161 cggtagacgt ctaccagggc ggcgcaggta cttccgtaaa catgaacacc aacgaagtgc
     5221 tggccaatat cggtctggaa ctgatgggtc accagaaagg tgaatatcag tacctgaacc
     5281 cgaacgacca tgttaacaaa tgtcagtcca ctaacgacgc ctacccgacc ggtttccgta
     5341 tcgcagttta ctcttccctg attaagctgg tagatgcgat taaccaactg cgtgaaggct
     5401 ttgaacgtaa agctgtcgaa ttccaggaca tcctgaaaat gggtcgtacc cagctgcagg
     5461 acgcagtacc gatgaccctc ggtcaggaat tccgcgcttt cagcatcctg ctgaaagaag
     5521 aagtgaaaaa tatccaacgt accgctgaac tgctgctgga agttaacctt ggcgcaacag
     5581 caatcggtac tggtctgaac acgccgaaag agtactctcc gctggcagtg aaaaaactgg
     5641 ctgaagtcac tggcttccca tgcgtaccgg ctgaagacct gatcgaagcg acctctgact
     5701 gcggcgctta tgttatggtt cacggcgcgc tgaaacgcct ggctgtgaag atgtccaaaa
     5761 tctgtaacga cctgcgcttg ctctcttctg gtccacgtgc cggcctgaac gagatcaacc
     5821 tgccggaact gcaggcgggc tcttccatca tgccagctaa agtaaacccg gttgttccgg
     5881 aagtggttaa ccaggtatgc ttcaaagtca tcggtaacga caccactgtt accatggcag
     5941 cagaagcagg tcagctgcag ttgaacgtta tggagccggt cattggccag gccatgttcg
     6001 aatccgttca cattctgacc aacgcttgtt acaacctgct ggaaaaatgc attaacggca
     6061 tcactgctaa caaagaagtg tgcgaaggtt acgtttacaa ctctatcggt atcgttactt
     6121 acctgaaccc gttcatcggt caccacaacg gtgacatcgt gggtaaaatc tgtgccgaaa
     6181 ccggtaagag tgtacgtgaa gtcgttctgg aacgcggtct gttgactgaa gcggaacttg
     6241 acgatatttt ctccgtacag aatctgatgc acccggctta caaagcaaaa cgctatactg
     6301 atgaaagcga acagtaatcg tacagggtag tacaaataaa gaaggcacgt cagatgacgt
     6361 gccttttttc ttgtgagcag taacttaaaa ataacaacct aatatcaact tgttaaaaaa
     6421 caaggaaggc taatatgcta gttgtagaac tcatcatagt tttgctggcg atcttcttgg
     6481 gcgccagatt ggggggaata ggtattggtt ttgcaggcgg attgggggtg ctggttcttg
     6541 ccgctattgg cgttaaaccc ggtaacatcc cgttcgatgt catttccatt atcatggcgg
     6601 ttatcgccgc tatttctgcc atgcaggttg ctggcggtct ggactatctg gttcatcaga
     6661 cagaaaagct gctgcgccgt aacccgaaat acatcacgat cctcgcaccg atcgtgacct
     6721 atttcctgac tatctttgct ggtactggca acatatctct ggcgacactg ccagttatcg
     6781 ctgaagttgc gaaggaacaa ggcgtcaaac cttgccgtcc gctgtctact gcagtggtat
     6841 ccgcgcagat tgcgatcacc gcatcgccaa tctcagcggc agtggtttac atgtcttccg
     6901 tgatggaagg tcatggcatc agctacctcc atctgctctc cgtggtcatc ccgtccaccc
     6961 tgctggcggt tctggtgatg tcctttctgg tcactatgct gttcaactcc aaactctctg
     7021 acgatccgat ttatcgcaag cgtctggaag agggcctggt tgaactgcgc ggtgaaaagc
     7081 agattgaaat caaatccggt gcaaaaacgt ccgtctggct gttcctgctg ggcgtagttg
     7141 gcgtggttat ctatgcaatc atcaacagcc caagcatggg tctggttgaa aaaccgctga
     7201 tgaacaccac caacgcaatc ctgatcatca tgctcagcgt tgcaactctg accaccgtta
     7261 tctgtaaagt cgataccgac aacatcctca actccagcac cttcaaagca ggtatgagcg
     7321 cctgtatttg tatcctgggt gttgcgtggc tgggcgatac tttcgtttcc aacaacatcg
     7381 actggatcaa agataccgct ggtgaagtga ttcagggtca tccgtggctg ctggccgtca
     7441 tcttcttctt tgcttctgct ctgctgtact ctcaggctgc aaccgcaaaa gcactgatgc
     7501 cgatggccct ggcactgaac gtttcaccgc tgaccgctgt tgcttctttc gctgcggtgt
     7561 ctggtctgtt cattctgccg acctacccga cgctggttgc tgcggtacag atggatgaca
     7621 cgggtacaac ccgtatcggt aaattcgtct tcaaccatcc gttcttcatc ccgggtactc
     7681 tgggtgttgc cctggccgtt tgcttcggct tcgtgctggg tagcttcatg ctgtaatgac
     7741 caatcgcggg gcgttcacgc cccgctttct ttcccgccga ctaacatcct tcccccgtcc
     7801 gttgtatagt gacctctctc ttgcggttcc atctgttctt gcgaggtgtt tatgcttgat
     7861 gaagaaagtt cgaataccgc gtctgtcgtg gtgctatgta ccgcaccgga tgaagcgaca
     7921 gcccaggatt tagccgccaa agtgctggcg gaaaaactgg cggcctgcgc gaccttgatc
     7981 cccggcgcta cctctctcta ttactgggaa ggtaagctgg agcaagaata cgaagtgcag
     8041 atgattttaa aaactaccgt atctcaccag caggcactgc tggaatgcct gaagtctcat
     8101 catccatatc aaaccccgga acttctggtt ttacctgtta cacacggaga cacagattac
     8161 ctctcatggc tcaacgcatc tttacgctga tcctgctact ttgcagcact tccgtttttg
     8221 ccggattatt cgacgcgccg ggacgttcac attttgtccc cgcggatcaa gcctttgctt
     8281 ttgattttca gcaaaaccaa catgacctga atctgacctg gcagatcaaa gacggttact
     8341 acctctaccg taaacagatc cgcattacgc cggaacacgc gaaaattgcc gacgtgcagc
     8401 tgccgcaagg cgtctggcat gaagatgagt tttacggcaa aagcgagatt taccgcgatc
     8461 ggctgacgct tcccgtaacc atcaaccagg cgagtgcggg agcgacgtta actgtcacct
     8521 accagggctg tgctgatgcc ggtttctgtt atccgccaga aaccaaaacc gttccgttaa
     8581 gcgaagtggt agccaacaac gcagcgtcac agcctgtgtc tgttccgcag caagagcagc
     8641 acaccgcgca attgcccttt tccgcgctct gggcgttgtt gatcggtatt ggtatcgcct
     8701 ttacgccatg cgtgctgcca atgtacccac tgatttctgg catcgtgctg ggtggtaaac
     8761 agcggctctc cactgccaga gcattgttgc tgacctttat ttatgtgcag gggatggcac
     8821 tgacttacac ggcgctgggt ctggtagttg ccgccgcagg gttacagttc caggcggcgc
     8881 tacagcaccc atacgtgctc attggcctcg ccatcgtctt taccttgctg gcgatgtcaa
     8941 tgtttggctt gtttactcta caactcccct cttccctgca aacacgtctc acgttgatga
     9001 gcaatcgcca acagggcggc tcacctggcg gtgtgtttgt tatgggggcg attgccggac
     9061 tgatctgttc accatgcacc accgcaccgc ttagcgcgat tctgctgtat atcgcccaaa
     9121 gcgggaacat gtggctgggc ggcggcacgc tttatctcta tgcgttgggc atgggcctgc
     9181 cgctgatgct aattaccgtc tttggtaacc gcttgctgcc gaaaagcggc ccgtggatgg
     9241 aacaagtcaa aaccgcgttt ggttttgtga tcctcgcact gccggtcttc ctgctggagc
     9301 gagtgattgg tgatgtatgg ggattacgct tgtggtcggc gctgggtgtc gcattctttg
     9361 gctgggcctt tatcaccagc ctacaggcta aacgcggctg gatgcgcgtg gtgcaaataa
     9421 tcctgctggc agcggcattg gttagcgtgc gcccacttca ggattgggca tttggtgcga
     9481 cgcataccgc gcaaactcag acgcatctca actttacaca aatcaaaacg gtagatgagt
     9541 taaatcaggc gctcgttgaa gtcaaaggca aaccggtgat gttagatctt tatgccgact
     9601 ggtgcgtcgc ctgtaaagag tttgagaaat acaccttcag cgacccgcag gtgcaaaaag
     9661 cgttagcaga cacggtctta cttcaggcca acgtcacggc caacgacgca caagatgtgg
     9721 cactgttaaa gcatcttaat gtccttggcc taccgacaat tctctttttt gacggacaag
     9781 gccaggagca tccacaagca cgcgtcacgg gctttatgga tgctgaaacc ttcagcgcac
     9841 atttgcgcga tcgccaaccg tgaacgacac ttgcagtggg aaagacggag gaaaatagcg
     9901 tgcaacgtga agatgtactg ggagaagccc tgaaattatt agaattacaa gggattgcca
     9961 acaccacgct ggagatggtt gctgaacgtg tggattatcc actggacgag ctacgccgct
    10021 tctggccaga caaagaggca atcctctacg atgcgctgcg ttatcttagc caacagatag
    10081 atgtctggcg tcgtcagctg atgctggacg aaacgcaaac cgccgaacaa aagctgctgg
    10141 cacgttatca ggcgttatcg gagtgcgtta aaaacaaccg ctatccgggc tgtctattta
    10201 tcgctgcctg tacgttttat cccgatcctg gccaccctat tcatcaactg gccgatcagc
    10261 aaaaaagcgc ggcctacgat ttcacccacg aactgttaac cacgctggaa gttgacgatc
    10321 cggcgatggt agcaaagcag atggaactgg tgctggaagg ctgtttaagc cgaatgctgg
    10381 tgaatcgtag ccaggcggat gtcgacactg cacatcggct ggcggaagat atcctgcgct
    10441 tcgcccgctg ccgccagggt ggtgcattga cctgacagaa acacagaaaa gaagcgattt
    10501 gccgcaatct taagcagttg aatcgctttt actgaaatta ggttgacgag atgtgcagat
    10561 tacggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
    10621 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
    10681 ggaggtccgt ttttcgtttc agaacaccat gatttaagtg ttcttcc
//
LOCUS       DAZFJF010000142        10590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFJF010000142 DAZFJF010000000
VERSION     DAZFJF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.143"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            124..1491
                     /gene="dcuC"
                     /locus_tag="ACHKAU_004166"
     CDS             124..1491
                     /gene="dcuC"
                     /locus_tag="ACHKAU_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5533121.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1534..2031)
                     /gene="sspB"
                     /locus_tag="ACHKAU_004167"
     CDS             complement(1534..2031)
                     /gene="sspB"
                     /locus_tag="ACHKAU_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO5533122.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2037..2675)
                     /gene="sspA"
                     /locus_tag="ACHKAU_004168"
     CDS             complement(2037..2675)
                     /gene="sspA"
                     /locus_tag="ACHKAU_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO5533123.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3069..3461)
                     /gene="rpsI"
                     /locus_tag="ACHKAU_004169"
     CDS             complement(3069..3461)
                     /gene="rpsI"
                     /locus_tag="ACHKAU_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO5533124.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3477..3905)
                     /gene="rplM"
                     /locus_tag="ACHKAU_004170"
     CDS             complement(3477..3905)
                     /gene="rplM"
                     /locus_tag="ACHKAU_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO5533125.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4124..5251)
                     /gene="zapE"
                     /locus_tag="ACHKAU_004171"
     CDS             complement(4124..5251)
                     /gene="zapE"
                     /locus_tag="ACHKAU_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO5533126.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5445..5843
                     /gene="zapG"
                     /locus_tag="ACHKAU_004172"
     CDS             5445..5843
                     /gene="zapG"
                     /locus_tag="ACHKAU_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO5533127.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5997..7364
                     /gene="degQ"
                     /locus_tag="ACHKAU_004173"
     CDS             5997..7364
                     /gene="degQ"
                     /locus_tag="ACHKAU_004173"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO5533128.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7454..8521
                     /gene="degS"
                     /locus_tag="ACHKAU_004174"
     CDS             7454..8521
                     /gene="degS"
                     /locus_tag="ACHKAU_004174"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO5533129.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8584..9522)
                     /gene="mdh"
                     /locus_tag="ACHKAU_004175"
     CDS             complement(8584..9522)
                     /gene="mdh"
                     /locus_tag="ACHKAU_004175"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5533130.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            9957..10427
                     /gene="argR"
                     /locus_tag="ACHKAU_004176"
     CDS             9957..10427
                     /gene="argR"
                     /locus_tag="ACHKAU_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO5533131.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2608 a         2691 c         2633 g         2658 t
ORIGIN      
        1 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
       61 catcattgtt ttcaatctgc cgtttatggg attgaccgtt ttcttttgac acggagttca
      121 acaatgttcg gcataattat atctgtcatc gtattaatta cgatgggcta tttgatcctg
      181 aaaaactaca aacctcaggt ggtgctggct gccgcaggta tcttcctgat gatgtgcggt
      241 gtctggttag ggttcggtgg tgtactcgat cccgccaaaa gcagcggcta cttgatcgtc
      301 gatatttata atgaaatcct gcgcatgctg tccaaccgca ttgccggatt ggggctgtcg
      361 attatggcag tgggcggtta tgcccgctac atggagcgca caggagccag tcgcgcgatg
      421 gtgagcttgc taagccgccc gttaaaactc attcgctcgc cgtatattat tctgtcggca
      481 acttacgtca tcggccaaat catggcgcag tttattacca gcgcctccgg cctgggtatg
      541 ttgctgatgg tcaccttatt tccgacgctg gtgagtctgg gagtaagtcg cctctctgcg
      601 gtggcggtta tcgcaaccac gatgtccatt gagtggggga ttctggaaac gaactccatt
      661 tttgcagccc aggtcgcggg aatgaaaatt gccacttact tcttccacta ccagcttccg
      721 gtcgcctctt gcgtcattat ctcggtggcg atctcccact ttttcgtgca acgcgctttt
      781 gacaaaaaag ataaaaatat caatcacgaa caggcagagc taaaagctct cgataatgtc
      841 ccgccgctct attacgccat tttacctgtg atgccgttaa tcttgatgct cggctcgctg
      901 ttcctcgccc acatcgggct gatgcagtca gaactgcatc tggtggcggt gatgttactg
      961 agtttgacgg tgacgatgtt tgttgagttc ttccgcaagc ataacttgcg cgaaacaatg
     1021 gacgatgtgc aggcgttttt tgacggcatg ggtacgcagt ttgccaacgt ggtaacgctg
     1081 gtggtcgcgg gtgaaatatt tgcgaaaggc ttaacgacga ttggcactgt tgatgcggtt
     1141 atcaggggtg cggagcattc tggtctgggc ggtattggcg tgatgattat tatggcgcta
     1201 gtcattgcca tttgtgccat tgtgatgggc tctggcaatg cgccgtttat gtcatttgcc
     1261 agtcttattc cgaatatcgc agccggacta catgtaccag cggttgtaat gattatgccg
     1321 atgcattttg ccacgacgct agcgcgcgcg gtttcgccga ttactgcggt ggtggtcgtt
     1381 acgtcaggaa ttgcaggcgt ttcgcctttt gcggtggtga agcgaacagc gatccccatg
     1441 gcagtcggtt tcgtggtgaa tatgattgcc acaatcacgc tattttatta agccattaaa
     1501 agacaaaaca ggccaccagg gcctgttttt gtattacttc acaacgcgta atgccggtcg
     1561 accaccgcgt ggtggctgcg gaggttcatc gtcaggatga gtgtcatcat cgtgatctgg
     1621 cttgtcgcca tcaataaccg acataacggt ttcgttgtct gccgatgcct cttcatcatt
     1681 catgatgctg gtatcttcat cgtaggcagc ttcaggctca aacatcgtgc ctgcgccatt
     1741 ttcacgggcg tagatagcca gcacggcagc cagcggcaca gaaacctgac gcggaatgcc
     1801 gccaaagcgc gcgttaaagc gtacctcatc attcgccagt tccagattgc cgacagcacg
     1861 cggcgcaatg ttgagtacga tttgcccgtc acgcgcatat tccataggaa cctgcacgcc
     1921 agggagcgtc acatccacca ccaagtgcgg cgtgagctgg ttatccagca accactcata
     1981 gaatgcacgc agcagatagg gacgacgtgg tgttagctgt gacaaatcca tacagattaa
     2041 ctccggccca gacgcatttc acgttctgct tcagttaaag aagcaaggaa agagtcacgc
     2101 tcaaagacgc gggtcatata gcctttcagc tctttcgcac ccgggccgct gaactcgatg
     2161 cccagttgcg gcagacgcca cagcagcgga gcaagatagc aatcgaccag gctgaactca
     2221 tcgctcagga agtacggctt ctgaccgaag accggcgcaa tcgccagcag ttcttcgcgc
     2281 agttgcttac gtgcggcatc tgcttcagaa gctgaaccgt tgatgatggt gttcatcagc
     2341 gtgtaccagt ctttttcgat gcgatgcatg tacagacggc tttcaccgcg agctaccggg
     2401 taaacaggca tcagtggcgg atgcgggaaa cgctcatcca gatattccat aatgatgcga
     2461 gattcccaca gggtcagctc acgatccacc agggtcggaa cgctctgatt cgggttgagg
     2521 tcaatcagat cctgaggcgg attgtccttt tccacgtgtt cgatctcgaa acttacacct
     2581 ttctcagcca gcacaatgcg gacctgatgg ctatagatgt cagtaggacc ggaaaacagc
     2641 gtcattaccg aacgtttgtt ggcagcgaca gccatgaaaa cctccaggta tagtcagaat
     2701 ttttactgct accagccacc aggtggccag tcagaagttg tgttacccag taaggaacga
     2761 ctctctttgt tcgaaaatca aacaaaaaat gagcaatacc cgacatttgg gcagaaaatt
     2821 ggatgatagt ttaccagatt ttgcgacctt tgtggtgagt cgatgccgga aatggggaaa
     2881 aagagatgcg ctttagtctg aaatagttga cttagtccct tattggcgat gtggtttttg
     2941 ttttgcctgt ctgtcagacg gtagcaaaaa gcaactttcc aatttttacg ctgattcaga
     3001 atttaaccat aaaaaacccg ccgaagcggg ttttttcgca aattgttttc tgccggagca
     3061 gaagccaatt aacgtttgga gaactgcgga cgacgacgtg ctttacgcag accgactttc
     3121 ttacgttcaa cctgacgagc gtcacgagta acgaagccag ctttacgcag ttcagaacgc
     3181 agggactcgt cgtattccat cagagcgcgg gtgataccgt gacggatcgc accagcctga
     3241 ccagagatac caccaccttt aacggtgatg tacaggtcca gtttctcaac catgtcgacc
     3301 agttccagcg gctgacgaac taccatgcga gcagtttcac gaccgaagta ctgttccaga
     3361 gaacgttggt tgattacgat tttaccgttg cccggtttga tgaaaacgcg agctgcggaa
     3421 cttttgcggc gaccagtgcc gtagtattga ttttcagcca ttgcctataa tcccgattag
     3481 atgtcaagaa cttgcggttg ctgtgccgcg tggttgtgct cgttacccgc gtaaactttc
     3541 agtttacgga acatagcacg acccagcggg ccttttggca acatgccttt aaccgcgatt
     3601 tcaatcacac gctcaggacg gcgagcaatc atctcttcaa aggtcgcttg tttgatacca
     3661 ccgatgtggc cggtgtggtg atagtacact ttgtcagtac gcttgttgcc ggttacagca
     3721 actttgtcag cgttcagaac gatgatgtaa tcaccggtat ctacgtgcgg agtgtattcc
     3781 gctttgtgct taccgcgcag gcgacgagcc agttcagtag ccagacggcc cagagtttta
     3841 ccggtcgcgt caacaacata ccagtcgcgt tttacggttt ctggtttagc tgtaaaagtt
     3901 ttcattaaaa gcttacccaa taaatagtta cacgttggtg aacacccaaa cgtcttcaat
     3961 tgttgaggtt cacacgacaa agtccggcaa acctacccct tcgaatagcc tatgccagca
     4021 cacaaaaagt tttgggaaaa aaactttctt gtaacgtggg gtcgcaggat tatagagaag
     4081 tcggggtcaa agatcgaccc ctttttgtga tttgtgacag gttttaaccc gccaaatgct
     4141 cgcgcttcag atactcttcg ctttgcatct cttgcagacg tgacaggcaa cgctggaact
     4201 caaacttcag ccgatcgccc tgataaattt catacagcgg cacttctgca ctcaccacta
     4261 atttgacatg gcgctcgtaa aactcatcca ccagcgcaat aaagcgccgc gcttcgctct
     4321 ccatcaaccg cgtcataact ggtacatcaa acaacatgac cgtatgaaag agacgtgaga
     4381 gcgcaatata gtcatgctga ctgcgggcgt cgacgcacag cgtagtaaaa gagaccgcca
     4441 gcgtctggtt ctcgacgccc attgtcgcca atggccgatg gttgatttct aacgtcggtg
     4501 aattttctcg tttcgccccc gccagcgcca accatagttt atccatctgc gcccgggttt
     4561 catcgttgag tggcgaaagc cacagatgcg cctgagtgag tgtacgcagt cgataatcaa
     4621 caccagcgtc cacgttcatt acatcacaat gctgtttaat ggcatcgatt gcaggcagaa
     4681 aacgcgcacg ttgcaggcca tttcgataaa gttcatccgg cggaatattt gacgtcgcta
     4741 ccagggtaat gccgcgggcg aacagggctt tcatcagacc gccaagtagc atggcgtcgg
     4801 taatatcaga aacaaaaaat tcgtcaaaac agagcacgtc agtttcggct ttaaagcgat
     4861 cggcaataat ttccagcgga tcggtctgcc cctgtaaggc agttagctct tcgtgcaccc
     4921 gcagcataaa acggtgaaag tgcaggcgct gtttccgctc tcccggcagg ctttgataga
     4981 aaaggtccat cagccaggtt ttcccgcgtc ctacaccgcc ccacatatat aagccacgca
     5041 ctggcgtatg ctttgtgtct tcgcgtttac cccacagctt accgacccgc gccattagcc
     5101 cactcgtcct gggggctggt ggcctgctat tgatgagttc ttgataaata atttccaggc
     5161 ggctgacggc ctctttttga acgtcgtcgg gttgatggct gccttcattg agcgccttca
     5221 ggtattgcga tgttggggta acgctttgca tgatcttatt gttattcctt gaataatcgg
     5281 tgcgccgttg ttcacggttg acgaaaaaaa ggccgttcta cactacgcga tatgcagtcg
     5341 ggattccact tctgtggaat taacggttat agtggcataa tcagccgcag gcatggagcc
     5401 tgaagccaac accctacgga aacaaaagac aacgggagat gttcatgacc tgggaatatg
     5461 cgctaattgg gttagtcgtc ggcatcatta ttggtgctgt ggccatgcgt tttggtaatc
     5521 gtaaactacg ccagcaacag gcgttgcagt acgaactgga aaagaataaa gctgaactgg
     5581 acgagtatcg cgaagagctg gttagccact ttgcccgcag cgcggaatta ctggatacca
     5641 tggcgcacga ctatcgccag ctgtatcagc acatggcgaa aagctccagc agcctgctgc
     5701 cggaactgtc tgctgaagca aacccgttcc gtaatcgtct ggcagagtct gaagccagca
     5761 acgatcaggc accggtgcag atgcctcgcg actattctga aggcgcatcc ggcctgctgc
     5821 gtactggcgc gaagcgcgac taatttattt ttcgggcgca gccattgcgc cctcctcttc
     5881 tctccctccc cgactatcat ttaatctggt gtctcattgt tagccgtctg aaaattcaat
     5941 aacatcaaac tgttttgaat ctcttttctt atcattcagg tacgagagca ggaataatga
     6001 aaaaacaaac ccagctgttg agtgcattag cgttaagtgt cgggttaact ctctcggcgt
     6061 catttcaggc cgtcgcgtcg attccaggcc aggttgccga tcaggcccct ctccccagtc
     6121 tggctccaat gctggaaaaa gtgcttccgg cagtggtgag cgtacgggtg gaaggaacgg
     6181 ccagtcaggg acagaaaatc ccggaagaat tcaaaaagtt ttttggtgat gatttaccgg
     6241 atcaacctgc acaacccttc gaaggtttag gttccggtgt catcatcaac gccagtaaag
     6301 gctatgtgct gaccaacaac catgtgatta atcaggcaca gaaaatcagt attcagctca
     6361 atgatgggcg cgagtttgat gcaaaactga ttggtagcga tgaccagagc gatatcgccc
     6421 tgttacaaat tcaaaacccg agcaaattaa cgcaaatcgc tattgccgac tccgataaat
     6481 tgcgcgtcgg tgattttgcc gtagcggtcg gtaacccatt tggccttggg caaaccgcca
     6541 cctctggcat tgtttccgca ttaggccgca gcgggttgaa tcttgaaggt ctggaaaact
     6601 ttatccagac agatgcttcc attaaccgcg gtaactccgg cggtgcacta ttaaacctta
     6661 acggtgagtt aattggcatc aacactgcaa tccttgcgcc tggcggcggg agcgtcggga
     6721 ttggatttgc catccccagt aatatggcgc gaacactggc gcagcagctt atcgactttg
     6781 gtgaaatcaa acgcggtttg ttaggcatca aaggcaccga gatgagtgcc gatatcgcca
     6841 aagccttcaa ccttgacgtg cagcgtggcg cgtttgtcag cgaagtgttg ccaggttctg
     6901 gctcggcaaa agcgggcgtc aaagcgggcg atattattac cagcctcaac ggcaaaccgc
     6961 tgaatagctt tgctgagttg cgctctcgta tcgcgactac agagccgggc acgaaagtga
     7021 agcttggcct gctgcgtaac ggcaaaccac tggaagtaga agtgacgctt gataccagca
     7081 cctcttcgtc ggccagcgct gaaatgatca cgccagcgct ggaaggtgca acgttgagcg
     7141 atggtcagct aaaagatggc ggcaaaggta ttaagatcga tgaagttgtc aaaggaagcc
     7201 cagctgctca ggctggcttg caaaaagacg atgtgatcat tggcgtcaac cgcgatcggg
     7261 tgaactcgat tgctgaaatg cgtaaagtgc tggcggcaaa accggccatc atcgccctgc
     7321 aaattgtacg cggtaatgaa agcatctatc tgctgatgcg ttaatgtcgt aaaccgggca
     7381 tcaggcttac gtgtgatgtc cggttaactc gtggtatgct gctgccgttc ccttttttaa
     7441 tgacgcctcc atcatgtttg tgaagctctt acgttccgtt gcgattggat taattgtcgg
     7501 cgctattctg ctggttgcca tgccttcgct gcgcagcctt aacccgcttt ccactccgca
     7561 atttgacagt accgatgaga cgcctgccag ttataatctg gcggttcgcc gcgccgcgcc
     7621 agcggtggtt aacgtttaca accgtggttt gaacaccaac tctcacaacc agcttgagat
     7681 ccgcaccctg ggatccggtg taatcatgga tcaacgcggt tatatcatca ccaataaaca
     7741 cgtcatcaac gacgccgatc agatcatcgt cgccttacag gatggacgtg tatttgaagc
     7801 attgctggtg ggatctgact ctctaaccga tctggcggta cttaaaatta atgccactgg
     7861 cggtttacct accattccga tcaatgcacg tcgcgtaccg cacattggcg acgtggtact
     7921 ggcgatcggt aacccgtaca acctcgggca gaccattacc caggggatta ttagtgctac
     7981 gggtcgaatc ggtctgaacc cgaccgggcg gcaaaacttc ctgcaaaccg atgcttccat
     8041 taaccacggt aactctggcg gcgcgctggt gaactcgctg ggagaactga tgggcattaa
     8101 cacgctgtcg tttgataaga gtaacgatgg cgaaacgccg gaaggtatcg gctttgcgat
     8161 tcctttccag ttagcaacca aaattatgga taagctgatc cgcgatggtc gcgtgatccg
     8221 cggctacatt ggtatcggcg gacgcgagat cgcaccactg cacgcgcagg gcggtggtat
     8281 agatcaactg caagggatcg tggttaatga agtgtcacct gacggcccgg cggcgaatgc
     8341 gggtattcag gtcaacgatc tgattatttc ggtggataac aaaccggcca tctctgctct
     8401 ggagacgatg gatcaggtgg cagaaattcg ccctggttcg gtgatcccgg ttgtagtgat
     8461 gcgtgatgat aagcagttaa cgctgcaggt caccattcag gaatatccgg caaccaatta
     8521 agtcgagtgc tcaaaacaaa aaaccggagt ctgtgctccg gttttttatt attcgctaat
     8581 taattactta ttaacgaact cttcgcccag ggcgatatct ttcttcagcg tatccagcat
     8641 accttccagt gcgctctgtt caaatgcgct cagggtaccg atagatttac gctcttccac
     8701 gccgttttta cccagcagca gcggttgaga gaagaaacgg gcgtactgac cgtcgccttc
     8761 aacgtaggca cattcgacaa cgccttgttc gccctgcagt gcacgaacca gagacagacc
     8821 aaaacgtgca gctgcctggc ccatagacag ggttgcagac ccaccaccgg ctttcgcttc
     8881 aaccacttca gtacctgcgt tctggatacg tttggtcaga tcagccactt cctgctcggt
     8941 aaaactaacg ccaggaacct gtgacagcag cggcagaatg gtaacaccag agtgaccgcc
     9001 aataaccggc acttcaactt cgcctggctg tttgcctttc agttccgcaa caaaagtgtt
     9061 ggaacgaatg atatccagcg tggtaacgcc gaacagtttg tttttgtcat aaacaccggc
     9121 ttttttcagc acttcagcag caatcgcaac tgtggtgtta accgggttag tgataatacc
     9181 aatgcacgct ttcgggcagg ttttcgcaac ttgctgtacc aggtttttca cgatgccggc
     9241 gttaacgtta aacaggtcgg aacgatccat acccggttta cgcgctacac ctgcagagat
     9301 aagaacgaca tctgcgcctt ccagcgccgg agtcgcatct tcaccagaaa aacctttgat
     9361 tttcacagca gtagggatat ggctcagatc gacagccaca ccgggagtca ctggagcgat
     9421 atcatacaga gagagttctg aacctgaagg cagttgggtt tttaacagta gtgcaagcgc
     9481 ctggccaata ccgccagcag cgccgaggac tgcgactttc atcctaaact ccttattata
     9541 ttgataaact aagatatgtt gctccgctgc cgcgacctta atccacaaaa ttgccgttta
     9601 caatgaccac atctcaagaa tgtgtagtca cgcaagttta gcgtttatgc atttaattgc
     9661 cgtaatcagg aacctaactt acgtaattaa cagtcgctta tgcattagcg caacattcca
     9721 acaggtggtg acaatatacc ctaccgttca gccaaaacaa catcaatttg ataacaatta
     9781 atttactttt aaacagaatt tgcatgccgt gacgcaggca tgtttctcaa taacgaaatt
     9841 tgataaaatc ccgctctttc ataacattat ttcagccttc ttcagggctg actgtttgca
     9901 taaaaattca tctgtatgca caataatgtt gtatcaacca ccatatcggg tgacttatgc
     9961 gaagctcggc taagcaagaa gaactagtta aagcatttaa agcattactt aaagaagaga
    10021 aatttagctc ccagggcgaa atcgtcgccg cgttgcagga gcaaggcttt gacaatatta
    10081 atcagtctaa agtctcgcgg atgttgacca agtttggtgc tgtacgtaca cgcaatgcca
    10141 aaatggaaat ggtttactgc ctgccagctg aactgggtgt accaactacc tccagtccat
    10201 tgaagaatct ggtgctggat atcgactaca acgatgcagt tgtcgtgatt cataccagcc
    10261 ctggcgcggc gcagttaatt gctcgcctgc tggactcact gggcaaagca gaaggtattc
    10321 tgggcaccat cgctggcgat gacaccatct ttaccacccc tgctaacggt ttcaccgtca
    10381 aagacctgta cgaagcaatt ttagagctgt tcgaccagga gctttaatct ctgccccgtc
    10441 gcttctggcg tcggggaaaa tgttgcttat ccctctcatt ccccttcttt ctcctgcgat
    10501 acatttaacg aatagtgcgt tttaccgcga cacatcattc acaaaatgaa tacatacggt
    10561 aaaaaaagca cattatgcaa aattcattat
//
LOCUS       DAZFJF010000143        10501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFJF010000143 DAZFJF010000000
VERSION     DAZFJF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.144"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(110..478)
                     /gene="ycgJ"
                     /locus_tag="ACHKAU_004177"
     CDS             complement(110..478)
                     /gene="ycgJ"
                     /locus_tag="ACHKAU_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO5533132.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            998..1693
                     /gene="minC"
                     /locus_tag="ACHKAU_004178"
     CDS             998..1693
                     /gene="minC"
                     /locus_tag="ACHKAU_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO5533133.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1717..2529
                     /gene="minD"
                     /locus_tag="ACHKAU_004179"
     CDS             1717..2529
                     /gene="minD"
                     /locus_tag="ACHKAU_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO5533134.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2533..2799
                     /gene="minE"
                     /locus_tag="ACHKAU_004180"
     CDS             2533..2799
                     /gene="minE"
                     /locus_tag="ACHKAU_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO5533135.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3171..3515)
                     /locus_tag="ACHKAU_004181"
                     /pseudo
     CDS             complement(3171..3515)
                     /locus_tag="ACHKAU_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3550..3669)
                     /locus_tag="ACHKAU_004182"
     CDS             complement(3550..3669)
                     /locus_tag="ACHKAU_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5533136.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3630..3815)
                     /gene="ymgJ"
                     /locus_tag="ACHKAU_004183"
     CDS             complement(3630..3815)
                     /gene="ymgJ"
                     /locus_tag="ACHKAU_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO5533137.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3916..4089
                     /gene="ymgI"
                     /locus_tag="ACHKAU_004184"
     CDS             3916..4089
                     /gene="ymgI"
                     /locus_tag="ACHKAU_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO5533138.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            4091..4435
                     /gene="ymgG"
                     /locus_tag="ACHKAU_004185"
     CDS             4091..4435
                     /gene="ymgG"
                     /locus_tag="ACHKAU_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO5533139.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4439..4774
                     /gene="ymgD"
                     /locus_tag="ACHKAU_004186"
     CDS             4439..4774
                     /gene="ymgD"
                     /locus_tag="ACHKAU_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO5533140.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4831..7152)
                     /locus_tag="ACHKAU_004187"
     CDS             complement(4831..7152)
                     /locus_tag="ACHKAU_004187"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5533141.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7269..7478)
                     /locus_tag="ACHKAU_004188"
     CDS             complement(7269..7478)
                     /locus_tag="ACHKAU_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533142.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(7878..8096)
                     /gene="ymgF"
                     /locus_tag="ACHKAU_004189"
     CDS             complement(7878..8096)
                     /gene="ymgF"
                     /locus_tag="ACHKAU_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO5533143.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(8228..9751)
                     /gene="pdeG"
                     /locus_tag="ACHKAU_004190"
     CDS             complement(8228..9751)
                     /gene="pdeG"
                     /locus_tag="ACHKAU_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5533144.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(10083..10331)
                     /gene="ymgC"
                     /locus_tag="ACHKAU_004191"
     CDS             complement(10083..10331)
                     /gene="ymgC"
                     /locus_tag="ACHKAU_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO5533145.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(10444..>10501)
                     /locus_tag="ACHKAU_004192"
     CDS             complement(10444..>10501)
                     /locus_tag="ACHKAU_004192"
                     /inference="COORDINATES: protein motif:HMM:NF022255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFO5533146.1"
                     /translation="DVIRKTLEIVLRYTADDM"
BASE COUNT         2995 a         2422 c         2250 g         2834 t
ORIGIN      
        1 taagtaaatg tcaaccgacg gggactctcc ccgtcataaa cgttaacgcc ccactcctct
       61 ctgtcgctca agaggttaat gacgaataga acataatgta aacaagacat cactcacgac
      121 acataaataa catctttgtg gtggtttgat tgatttttcc actccgctta ccatctacac
      181 caaaataacg atcatcacga cataattttt ctttaacatc gcaaaataca ccgttagcga
      241 atgtaaattc gctgggatcg tagccttgta atgattgtaa gttttcagcg gctttttcgc
      301 cgagatattt ccttgttaac tctggggaaa tgccatctga gttcacacaa atatacgggt
      361 cgcaaacaac gccgcacgcc ggagattgaa gtccgctagc gtcatgtcca gacgccattg
      421 atgagaacat catccccaca cctgacaatc cgatataaat aatacgcatc atattcatag
      481 cacatcctga taatccaacc gtacaacact tatgctcatt tcgtcatcaa tccgtaatca
      541 aaaccgctga cctggacgag ttacgatgac ctgacagaag tatcagacgt cttctgcggt
      601 ttctatgata gagcaattct gtggcggata ttaagataac gggtatctgt tctttaatga
      661 tgccgcaaaa aatggaaagt taagaaagcc tggcgtggtg aaaaaataca cttgcctcaa
      721 tactggatat ttaaacaggt ctggagatgc tgtcacgatt agggtgcgga aaaaggaagc
      781 gtaaataaga agaatcaaaa gtacgacggc aatgggttga ttgacaaggg tattttttaa
      841 gctatgaatc agcgccattt atcacagaat agacttttac tctgaataaa tgggagggtg
      901 acttgcctca atataatcca gactataaca tgccttatag tcttcggaac atcatcgcgc
      961 gctggcgatg attaatagct aattgagtaa ggccaggatg tcaaacacgc caatcgagct
     1021 taaaggcagt agcttcactt tatctgtggt tcatctgcat gaggcagaac ctaaggttat
     1081 ccatcaggcg ctggaagaca aaatcgctca ggcccccgca tttttaaaac atgcccccgt
     1141 tgtactcaac gtcagtgcac tggaagaccc ggtaaactgg tcagcgatgc ataaggcggt
     1201 ttcggcaacc ggtttgcggg ttattggcgt aagtggctgc aaagatgcgc aacttaaagc
     1261 cgaaattgaa aaaatggggc tgcctatcct gacggaagga aaggaaaaag cgccacgtcc
     1321 agctcccgca ccgcaggctc cagcgcaaaa tacaacgccg gtcacaaaaa cgcgtttaat
     1381 agataccccg gtgcgttccg gtcagcgtat ttatgctcca caatgtgatc tgattgttac
     1441 aagccacgtt agcgctgggg ccgaattgat tgccgatggt aacattcatg tctatggcat
     1501 gatgcgcggt cgtgcgctgg caggggcaag tggtgaccgg gaaacgcaaa tattttgtac
     1561 gaacctgatg gcggaactgg tgtccatcgc aggtgaatac tggctgagtg atcaaatccc
     1621 agcagaattt tatggcaaag cggcgcgact gcagttagtc gaaaacgctt tgaccgttca
     1681 accgttaaat tgatcccttt ttaacaagga atttctatgg cacgcattat tgttgttact
     1741 tcgggcaaag ggggtgttgg taagacaacc tccagcgcgg ccatcgccac tggtttggcc
     1801 cagaagggaa agaaaactgt cgtgatagat tttgatatcg gcctgcgtaa tctcgacctg
     1861 attatgggtt gtgaacgccg ggtcgtttac gatttcgtca acgtcattca gggcgatgca
     1921 acgctaaatc aggcgttaat taaagataag cgtactgaaa atctctatat tcttccggca
     1981 tcgcaaacac gcgataaaga tgccctcacc cgtgaagggg tcgccaaagt tcttgatgat
     2041 ctgaaagcga tggattttga atttatcgtt tgtgactccc cggcagggat tgaaaccggt
     2101 gcgttaatgg cactctattt tgcagacgaa gccattatta ccaccaaccc ggaagtctcc
     2161 tcagtacgcg actctgaccg tattttaggc attctggcgt cgaaatcacg ccgcgcagaa
     2221 aatggcgaag agcctattaa agagcacctg ctgttaacgc gctataaccc aggccgcgta
     2281 agcagaggtg acatgctgag catggaagat gtgctggaga tcctgcgcat caaactcgtc
     2341 ggcgtgatcc cagaggatca atcagtattg cgcgcctcta accagggtga accggtcatt
     2401 ctcgacatta acgccgatgc gggtaaagcc tacgcagata ccgtagaacg tctgttggga
     2461 gaagaacgtc ctttccgctt cattgaagaa gagaagaaag gcttcctcaa acgcttgttc
     2521 ggaggataag ttatggcatt actcgatttc tttctctcgc ggaagaaaaa cacagccaac
     2581 attgcaaaag aacggctgca gattattgtt gctgaacgcc gtcgcagcga tgcagaaccg
     2641 cattatctgc cgcagttgcg taaagatatt cttgaggtca tttgtaaata cgtacaaatt
     2701 gatcctgaga tggtaaccgt acagcttgag caaaaagatg gcgatatttc tattcttgag
     2761 ctgaacgtga ccttaccgga agcagaagag ctgaaataag cccgctgtaa aaacgcattt
     2821 atcttcaagg cagagtcatt tctgccttga gtttttcatc cctctcatcc acgttgtggt
     2881 aaagcggcga gtattcttgc tgatactcct cattgctatt tccacctccc cctttacacc
     2941 ttaaggctgt tcccacgcac agaacaaaat gtttatgcct ggttgagtaa ataacctcat
     3001 tgttagtgtg gatacactgg gggatgataa ccccgcaatt tcattattca ttgttctccc
     3061 ataggatgag gtttcccgcc gtaactggcg ggcttttttt gccccagaat tttccctttt
     3121 agccccctgt aaacgaaaac tgcgccgaag cgcagtttaa tcgttgatgg tcagaacgac
     3181 cagttcacac cagccaccgc attccacggg gattccacac cggcaccatg gctatacccc
     3241 accccgaaat gcccgcttaa cgtactgctg aatgaggctt taatacctgc ctggtatatt
     3301 ccacgtctgc ccgacaaatc attgacaaaa ttaccgtcac tattcacttt cacccgatta
     3361 tcatcgacaa attctttgct cacagccgcc ttcaggcagg gttcaatttc cataccgttc
     3421 cccagacgca tgttgtaact cagcgttgca cccagttcac gatatatact gcgggtatcg
     3481 actgatttcg attccatgcc attggataaa tgatagtagc gtctaagtga cctaagctct
     3541 gctgcaacat cagcccgcag gcaccagacc aggggattca tcctgaagag acagcgcagg
     3601 tgtattgtgt tcaccgctca ccaaagacat catgatgaaa tgatgatatc ccgcataaga
     3661 aagaggcatt ttttacacgc agtgcgctga agtgtagttg gataaaaaat cactcttttc
     3721 agataatact gtcgtacgct tatctttcga caatgaggcc gtcggcaaaa taaaatgact
     3781 aatgtaatcg ttgctgatga atatcttctg ctcacataaa aatcacacaa taatattgat
     3841 aaatcagatt atcttacctt tacaacattg cgttaacagt caatgacact tcttgaataa
     3901 aaatattgtc aatcaatgag ttattcctca tttaagatta ttctcattca cgtaaaaaac
     3961 ataattccta tcatcacagc cacgctcatt ttgaattatc tgaataatag cgagcgttct
     4021 ttagttaagc agatcttaat tgaagacgaa attattgtgt gtgcaactta cttaataccg
     4081 gacatctaag ttgaagaaaa aaatacttgc cttcgggctt atctcagcat tgttctgctc
     4141 cacgccagcg atggcggata tgaatcgcac cacgaaaggt gcattgctag gcgcaggcgt
     4201 tggtttactt actggcaatg gtgttaatgg cgtactgaaa ggtgcagctg ttggcgctgg
     4261 tgttggtgca gtaacagaaa aaggccgcga cggtaaaaat gcgcgtaaag gtgcaaaagt
     4321 aggtgccgcc gttggtgctg tcactggcgt tctgaccggc aatggcctcg aaggggcgat
     4381 taaaggtgcc gtgattggcg gtacgggtgg tgcaattctg ggtaaaatga aataaggaat
     4441 gatgatgaaa aaatttgctt tactggcggg cctgtttgtt ttcgcaccaa tgacctgggc
     4501 gcaggactac aatattaaaa acggtttgcc gtcagaaacc tatattactt gcgcagaagc
     4561 caacgaaatg gcaaaaactg acagcgcaca ggttgcagaa attgttgcgg taatgggtaa
     4621 tgccagcgtt gccagccgtg atttaaaaat tgagcaatct ccggaactga gcgccaaagt
     4681 ggtagaaaaa ctgaatcagg tttgcgctaa agacccgcaa atgctgttga tcacggcaat
     4741 tgatgacacc atgcgtgcta tcggcaaaaa ataatatttc atatgtaccg cgtcgatgaa
     4801 tacgacgcgg ttttttagct aacggcggga tcagaactta tatttcaggc ccaccatcac
     4861 tgcggtgtca ttgtagccat tatcacccag ctgcacgccc acattccccc agacgctggc
     4921 cgctggattc aggttgccat ttacgccaag tttaatttcc cccaaattac tcgccccatc
     4981 ttgttcgact ttcacaccgt tcattgagac agcaaaatct ttactgttat ggatccagtt
     5041 catttcaaca aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg
     5101 aataaaggtg cggaaaccaa gtcgggtctg gatgttgtta tttgcgtcat tctcaatacg
     5161 tgagccattg gcttcggtgt gcgcattatg atcgacgccc atccagatag cctgtgcctg
     5221 tggctggaat atccacgtgt atttcgccgc ttccagacca aaggatttat tcagggcaaa
     5281 gccgtaaccc gcttccagcg agacggttgc gcctttcgca ctataggatt cataggctaa
     5341 ttcatcccct ttcaccgagt ttttaaacca gctatattgc gcccaggcgt caatgtatgc
     5401 gcctttttta ctgatgtcat cggcaaacca agtggcatac agccctgcgc aatagcctct
     5461 gacactccct ttcgaacgat aatccgacac gctggaatga gttgagttgt agtcgcgggc
     5521 ataaccagcc atcactccca aacgccaact gtcactgtcg gtaaaaccac cggttaacag
     5581 ctcggcccct aactgcgaga cgtagcgatg cgaggtcgtt ctcaactgtc cgttgctgtc
     5641 acgccaggca ttatgcccgc caatttgacg tagccaaagt cggcttgaac gctcctgttc
     5701 agttacagga tcgatgtagc gcatttcacc cgcacggtcg ttcagacgca taacaaaaag
     5761 agtgttagcg gctgccaggt tggcgacgta acttccagct tcgaggcgca ggacgggggt
     5821 aggcgcaaca gtaggcgctg aacttcctcc ggagtttgtt tcaccgccgc tcgttgcctg
     5881 gaagttagtc agataccagt ttttattggt cgcttcggta ttacctcggg ttaatgaata
     5941 ttcgtaggca ccggcgaaaa tcctcgaatc cttaataaat tccccatttg attccccctc
     6001 aacgctgata atctctatcc catttaacgt gtaagctccg ctgcctcctg cattggtaac
     6061 tttaactcgg gttgttccag aggtatttcc attaataacg actttatccg tagctgaatt
     6121 atcatcaccc aattcagttc tgagatgcaa tacgccatta ttaccaacgt aattttcagt
     6181 tattgttaat cttgttggtt cgacatcccg tccatctgcc cgggaaatat agaccgtact
     6241 attatcaacc gtgaggttgc tcaccgttga gtcagcttta atatcccagg tactattatc
     6301 tgaaagcgac agataggtat gagtgttaac atctgttgaa atattagctg aacccgttat
     6361 ttttgaatta gttgcatcga cggtcaaatg acctttaata ttattgataa aaacatctgc
     6421 agccgatgat acctcactat ccgtaatggt cacgttactc gtctcatttt tatggtagag
     6481 gatttcattt gctgacccta gtgagctacc agataaaata tctaccgttg cttcctgacc
     6541 atttatggaa gactccagca caattccaga cccctgatgc gcgactgcag atgtattttt
     6601 cagcgtcacc gtgccgccat tataaatttt agcagtcgcg gcgtcaggac ccgttgtata
     6661 aatttctccc tggttaaatg aaactgtccc ctggcgggca gcgatggcat gagcattgtc
     6721 cccatgcgtg gtgatatcaa catcggttaa tgtaacgctg gcattggtat aatcaagcgt
     6781 gtcaccagcc ttgacgacag tgccattagc gtacgcggca taactttctg ttccgtgggt
     6841 tgtgatctcc ccttttgcca tatccagttt tgctatggca ttattttcat tacttcctgt
     6901 atcggtagta ctttcttcat caagagaaga tacacacgca cgcgcgcttc cgggagatgc
     6961 acacgcaaga acgccgactg catgattacc cgcagtaaca ataaatgtat catctccgtc
     7021 agaaaaagag ggggtaaccg aaccgtcaga gttgactctt attatggtgt cagtattcag
     7081 gtttccactc gtttgactta gcaaaccaat tgagtaatcc cccgttatga caatcgtgtt
     7141 tttaccctgc aaaataacag caccgccaaa ttcacttacg attgcgctag ctgttaatgc
     7201 tgtgcttaga ggactattgt cagaaggata taaaccgccc gttgttacaa tgacgccatt
     7261 gttaatggtt aacttgctac cctctcctgc aacggatacc cgtaacttcc attacccgtt
     7321 gttataacta gcgcattatc aatatctttt acttttccat cttttattgc cacagcatgt
     7381 ttatattcat cgtgctgaat aaactgttca gcatatgcat tacccaccgc cagagcaata
     7441 agtcccaaac taaacccgga gagttttttc agtttcatag attaaccatc cattcattgg
     7501 attaacaaac attcctggtc aaataaccta tgcgttcaga ttaccaaaac ggagggtaaa
     7561 gacaaaaaca aaagcacaaa aaatttaaat agataaacag cgacaatata taaatgataa
     7621 ataagtcggc attatcattt ttcaccttat tttcatgaca ttgatcactt tgaggtgacg
     7681 attttgttta acaaataact cttcgacatc acaatcattg atagttgcaa taaaaattat
     7741 ttcaatgaca tataaaaaca actgtgataa caccagggtt gacaactatt tgcgcaataa
     7801 ttcctttact tcaatgatcc aggcattcac tgccagcaac ccattcagcc gcatactgac
     7861 agtgagaaac tgaaagatta ttgctgatcc ctgtggctga cgagtctttc ccttaatcga
     7921 ttaaccagat tgatcgccag ccaaacaagg gcattaagca aatttatcgt cacaagcact
     7981 tctcccagcc cggtactggg ttttgcaata aggatgacca ggagggtgat cagcataaat
     8041 gcaatggaaa agatgatata gagagtgaaa taatccagat tattactatt gttcatgcta
     8101 ctaatggcaa tcctgtcaaa actttcaaac aattatagca aacaaaataa tgaacaatag
     8161 taacaagagg caacgagcta attagctgtc tataattaca ccgcactatg gccttactga
     8221 tcaattttca ctcaaccaca accttcacct tcggtttaga aaggataatt ttgaccaggt
     8281 cgatgtatgt aacaggttta tagaaataat aaccctgctg aaatgtgata ttattttggt
     8341 tcagatagtc aagttgttca ttcgtttcga caccttcagc gacgatactg atcgaaagtt
     8401 tacgcgccag atccaataca caatccagaa ttcgggttga ttctgggtct gcgtttacgc
     8461 ggccaacgaa actgtgatca attttgatat aatcaatatg caggtcatga agataagaaa
     8521 gccctgagta gccagtaccg aaatcatcca gcgcgatgac aaaaccattt tcatgcagaa
     8581 tgttcaaccg ctgaaccaga ctttcatcaa cattcaatgg ctcacgctcg gtgacttcca
     8641 gaacaaggtt taaatcgcgg cgggtaaaac tgtcacggaa atttaaacac tcgtcgacaa
     8701 acgtcggcga aataatatgc gaagcgctaa aattaattcc aatatgaaaa ccttccggca
     8761 atttactcgc gatagcgttc atctgtctgg caacctgatt catcaggctt tgcgtaagcg
     8821 gaacgattaa tccggatttt tcagcaagtg gaataaatgc cgcgggtgat atatatccac
     8881 cgtgaggttg tttccagcgg gctaacacct caactccccg caatgtccct tcccgaccat
     8941 ttaccacagg ttggtaaaaa gggacgatct ccccattatt tattgcccgt cgcaggatct
     9001 cttcaggcgt tgcgctttta ttgaaatatc tatcaagcag gaaggcagcg gcgcaggcga
     9061 ttaacagaat aaatattaaa atcccaaagc ctttatcgat aagtctgtag aagctaaaga
     9121 gtggcggcgg gttaaagata atagtgaaat gatattttgt ggagtgaacc ttctcggtat
     9181 agggataatg tccattaagc gtcatcacat cacccgttgg tccaataatg tcatccgcca
     9241 cgcgtaatac ataccttacc cctttcaagg gtacattaag tgccccacga atatgctgat
     9301 cgcttatcgt aaccaaaatg cgcgtatctg caaattggtt ctgatagagc aggataggta
     9361 atctattaac ggtgtcgatt gctggagcca acagtaaatt actgtccggg aaaacaggaa
     9421 tacgagacaa caggacccga ttcccaggca gggatgtaca ccagactatt ccctgtttga
     9481 gaatgatgat tgtgcgcagg tgaggtttca gagctgcttc agtgccaagc tgatattgct
     9541 cctcgaggtt gcattccttc ccggcaatgt taaccgctgt ttgagtcgct tgtgacgctt
     9601 cttcaagtat atgattgata ttgttggcgg catatctcgc tcccgccagg tactctgctt
     9661 ttgcggaata ccagagctgg atgttcaaaa tagcgacacc ggtaataaac aagcatatcg
     9721 caacgaggat gggtatgagt gtattgcgca tttgcttgtc ccggccaaag ggtttaatac
     9781 atgaaaatgc cattcattcg ctcgcgctgg catctacaga gagcatggta gagagcataa
     9841 gaaatatcca cgccgaagat aaaattttct ccatcttgat ctaacccatt aattattgag
     9901 cataatgtag gcagtacaaa ataagttagg cgggatatca ggcgtcaaga atggagagcg
     9961 agaactctcc attcttgaca cctgatattg cggacataat aagaaagcat aacgcctgaa
    10021 atgctcactt tgcatcagca tggtgataca gctgatgttt attctaaaac cttactcaag
    10081 ttctaagaga gcacggattc cctgtcatta ataatgatat ccgttagtgt agtcttatgc
    10141 atttcaatgt actgcaatat ttgcagatag agatcagaaa gaatcacact ggtttcatct
    10201 tttaaacgca agcgtaaagc ctgatcaata atatgactat caacataccc atgcgtcatg
    10261 aacacctccc tctcgagatt tttaaacaaa gcacattgaa aaataaatct ctctgggatt
    10321 gaattattca tgtcagcctc gtagaaatat taacccaata aaattatgct gtgtatagtc
    10381 taaagcgaca gacaaaaata agatgaatga gttttctata aacttatact taataattag
    10441 aagttacata tcatcagctg tgtatcgcaa cacgatttcc agtgtttttc taatcacatc
    10501 a
//
LOCUS       DAZFJF010000144        10403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFJF010000144 DAZFJF010000000
VERSION     DAZFJF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.145"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..206)
                     /locus_tag="ACHKAU_004193"
     CDS             complement(<1..206)
                     /locus_tag="ACHKAU_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533147.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGP"
     gene            435..1043
                     /gene="yibF"
                     /locus_tag="ACHKAU_004194"
     CDS             435..1043
                     /gene="yibF"
                     /locus_tag="ACHKAU_004194"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO5533148.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1141..2532
                     /gene="selA"
                     /locus_tag="ACHKAU_004195"
     CDS             1141..2532
                     /gene="selA"
                     /locus_tag="ACHKAU_004195"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO5533149.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2529..4373
                     /gene="selB"
                     /locus_tag="ACHKAU_004196"
     CDS             2529..4373
                     /gene="selB"
                     /locus_tag="ACHKAU_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO5533150.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4563..5714
                     /gene="yiaY"
                     /locus_tag="ACHKAU_004197"
     CDS             4563..5714
                     /gene="yiaY"
                     /locus_tag="ACHKAU_004197"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO5533151.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            5879..7417
                     /gene="aldB"
                     /locus_tag="ACHKAU_004198"
     CDS             5879..7417
                     /gene="aldB"
                     /locus_tag="ACHKAU_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO5533152.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(7636..7839)
                     /locus_tag="ACHKAU_004199"
     CDS             complement(7636..7839)
                     /locus_tag="ACHKAU_004199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533153.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            7962..8285
                     /gene="yiaW"
                     /locus_tag="ACHKAU_004200"
     CDS             7962..8285
                     /gene="yiaW"
                     /locus_tag="ACHKAU_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5533154.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            8291..9427
                     /gene="yiaV"
                     /locus_tag="ACHKAU_004201"
     CDS             8291..9427
                     /gene="yiaV"
                     /locus_tag="ACHKAU_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5533155.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(9424..10398)
                     /gene="yiaU"
                     /locus_tag="ACHKAU_004202"
     CDS             complement(9424..10398)
                     /gene="yiaU"
                     /locus_tag="ACHKAU_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5533156.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2485 a         2540 c         2844 g         2534 t
ORIGIN      
        1 gggccgggca gggcgatgtc ggtttcaccg ggaaggacct ttgcaccgag caggggattg
       61 accggatggc cggacgtcac tccgccgggg cacaccgaac aggccacgcc ggtgggggca
      121 ccaatgcgca ccccggctga accctgaaca atgctaccgc catactgcgt catgtcgccc
      181 tgacgcgccg ccggttttcc gctcatctgc tcatccttgt tttatcgggt tatttgtctg
      241 cttccaggca aaaccctaca gatttataag aaagccttaa gtgagatatc catcagatag
      301 aaacaattag gaaaatattt atatcaccat gataaatatg tgatttattt ggaagtaaca
      361 aaccatcaac attttctgcc ctttttccca tcaccaggcc atactgatat ttttcctcgc
      421 acaaaggact cggcatgaaa ctcgtcggta gctacaccag cccgtttgta cgcaaacttt
      481 ctattctgtt gttagaaaag ggcataactt tcgaatttat taatgaactg ccctataacg
      541 cggacaacgg cgtggcgcaa tttaacccgt taggaaaagt gccggtgctg gtgaccgaag
      601 agggcgaatg ttggtttgat tcgccgatca tcgctgaata tattgaatta atgaatgtcg
      661 ctccggcgat gttgccgcgc gatccgctgg agtcgttgcg ggtgcgcaaa attgaggcac
      721 tggcggatgg cattatggat gccgggctgg tatcggtgcg tgaacaggcg cgtccagcgg
      781 cgcagcagtc tgaagatgaa ttgttacgcc agcgggagaa aatcaaccgc agtctggatg
      841 tgctggaagg atatctggtc gatggcacac tcaaaaccga tacggtcaat ctggcgacta
      901 tcgccattgc ctgtgccgtc ggatatctca atttccgccg cgttgcgcca ggctggtgtg
      961 tcgatcgccc acacttagtc aaactggtcg aaaacctgtt tagccgcgaa agttttgctc
     1021 gcaccgaacc gccaaaggct tgatgcgcga tatgtcctcc tgacccatct cacgttacaa
     1081 tccgtggtta tgttaaacgc ccttctccgt gtgagagggc cttgatcagc caggtttcct
     1141 atgacaaccg aaacgcgttc cctctatagt caacttccgg ctattgatcg cttattgcgc
     1201 gatagctcct tcctttcttt gcgtgatact tatggtcaca cccgcgtggt ggaattgttg
     1261 cgtcagatgc tcgacgaagc gcgagaagtg attcgtggca gccagacgct gcctgcgtgg
     1321 tgtgaaaact gggcgcaaga agtcgatgcc cggttgacga aagaagcgca gagcgcgctg
     1381 cgtccggtga tcaacctgac gggaaccgtg ctgcatacca accttgggcg agctttacag
     1441 gcggaagccg cggtggaagc cgttgcgcag gctatgcgtt cgccagtgac cctcgagtac
     1501 gatctggacg acgctggacg cggacatcgc gatcgtgcgc tggcgcagtt gctgtgccgc
     1561 attacggggg cggaagatgc ctgtatcgtt aataacaatg cggcggcggt gttattgatg
     1621 ttggcggcca ctgccagcgg gaaagaggtg gtggtttctc gcggcgaact ggtggagatt
     1681 ggcggcgcgt ttcgtattcc cgatgttatg cgtcaggcag gctgcaccct acacgaagta
     1741 gggaccacca accgcacgca cgcgaatgat tattgtcagg cggtgaatga aaataccgca
     1801 ctgttgatga aagtacatac cagtaactac agcattcagg ggttcaccaa agcgatagat
     1861 gaagcggaac tggtggcgct cggcaaagag ctggatgttc ccgtagtgac tgatttaggc
     1921 agtggctcgc tggtcgatct tagccagtac ggtttgccga aagagccaat gccgcaggag
     1981 ttgattgcgg cgggcgtcag tctggtgagt ttctccggcg acaagttgtt aggcgggccg
     2041 caggcaggaa ttattgttgg taaaaaagag atgatcgccc gcctgcaaag ccacccgctg
     2101 aagcgtgcat tacgcgcgga taaaatgacc ctcgcggcgc tggaagccac gttgcgtctt
     2161 tatttacacc ctgaagctct gagtgaaaaa ttaccgaccc tgcgcctgct tacccgcagc
     2221 gcagaggtca ttcaaatcca ggcacaacgt ttacaggccc cccttgccgc acattacggc
     2281 gcggagtttg cggtacaggt tatgccatgt ctttcgcaga ttggcagtgg ttcgctgccg
     2341 gttgatcgcc tgccgagcgc ggcattaacg tttacacccc atgatggacg cggtagccac
     2401 cttgagtcat tagccgcccg ctggcgtgaa ttgccagtgc cggtgattgg tcgtatttat
     2461 gacggacgat tgtggctgga tttacgctgc cttgaagatg agcaacggtt tttggagatg
     2521 ttgttgaaat gattattgcg actgccggac acgttgacca cggcaaaaca accttattgc
     2581 aggcgattac tggcgtaaat gctgaccgtc tgccggaaga aaaaaagcgc ggcatgacca
     2641 tcgatctcgg ctatgcctac tggccgcagc cggatggtcg cgtgcctggt tttatcgacg
     2701 ttcccggtca tgaaaaattt ctttccaaca tgctggcggg cgttggtggt atcgatcacg
     2761 cgctgttggt ggtggcgtgc gatgacggtg tgatggcaca gacccgtgag catctggcga
     2821 ttttgcagct gaccggtaac ccgatgctga cagtggtgct gaccaaagcc gatcgcgtgg
     2881 acgaagcgcg tgttgatgag gttgaacgtc aggtaaagga ggtgctgcgg gaatacggtt
     2941 ttgctgaggc aaaactgttt atcaccgcag caaccgaagg tcgaggaatt gatgccctgc
     3001 gcgagcatct gcttcagtta ccagaacgtg aacacgccag ccaacatagt ttccgcctcg
     3061 cgatcgaccg cgcatttacc gtaaaaggtg ccgggctggt cgtcaccggt acggcgttaa
     3121 gcggggaagt gaaggtaggc gattcactat ggctaactgg tgtaaataaa ccgatgcgtg
     3181 tacgtgcgct gcatgcacaa aaccagccaa cagaaaccgc ccatgccggg cagcgtatcg
     3241 cgcttaacat cgcgggtgat gcggaaaaag agcagattaa ccgtggcgac tggctgcttg
     3301 ccgatgcgcc gccagagccg ttcacacggg tgattgtcga gcttcaaacc catacgccgc
     3361 tgacccagtg gcagccgctg catattcacc acgccgccag ccacgttacg ggacgcgttt
     3421 cgctgctgga agataacctt gccgagctgg tgttcgacac gccgctgtgg ctggcggata
     3481 acgatcgctt agtgctgcgc gatatctcgg cccgcaacac gctggcgggg gcgcgtgtgg
     3541 tgatgcttaa cccgccgcgt cgcggtaaac gtaagccgga atatctgcaa tggctggcgt
     3601 ctcttgcacg ggcgcagagc gatgccgatg cgttatctgt tcatctggaa cgcggcgcgg
     3661 ttaaccttgc ggatttcgcc tgggcgcgcc agctcaacgg cgaagggatg cgcgaattgc
     3721 tgcaacagcc tggttatatt caggctggtt atagcttgtt gaatgcgccg gttgccgccc
     3781 gctggcagcg gaaaattctc gacacattag cgacttatca tgagcaacat cgcgatgaac
     3841 ctggtcctgg gcgcgaacgt ctgcgacgta tggcgttgcc aatggaagat gaagcgctgg
     3901 tactgttgct gattgaaaag atgcgcgaaa gcggcgacat cctcagtcat cacggttggc
     3961 tgcatctgcc ggatcacaaa gcgggcttca gcgaagagca gcaggccatc tggcaaaaag
     4021 cagagccgct gtttggtgac gagccgtggt gggtgcgtga tctggcaaaa gagacgggta
     4081 ccgacgagca ggcaatgcgc ctgacgctac gccaggcggc gcagcaagga ataattaccg
     4141 cgatcgtcaa agatcgttat taccgtaacg atcggattgt cgaatttgcc aatatgatcc
     4201 gcgatctcga tcaggagtgt ggttcaacct gcgcggcgga tttccgcgat cgcttaggcg
     4261 taggccgaaa gctggcaatt cagattctgg aatattttga ccgcattggc tttacgcgtc
     4321 gtcgtggaaa tgatcattta ttgcgcgacg cattattatt tccggaaaaa taagaaaatg
     4381 attaattaat ttttaaaata aattaataca aatttcttat gattttaaaa aaagcacatt
     4441 gtttaatgaa tacaatgtgc tttttattag attaattttg ataattaata ataatgatca
     4501 tgttttcgaa tgaaaatcct cagtaagctg cccacccttt tttacacttt caggagtgtg
     4561 ttatggcatc ttcaactttc tttattcctt ctgtgaatgt catcggcgct gattcattga
     4621 ctgatgcaat gaatatgatg gcagattatg gatttacccg taccttaatt gtcactgaca
     4681 gtatgttaac gaaattaggt atggcgggtg atgtgcaaaa agcactggaa gaacgcaata
     4741 tttttagcgt tatttatgat ggcacccaac ctaaccccac cacggaaaac gtcgccgcag
     4801 gtttgaaatt acttaaagaa aataattgcg atagcgtgat ctccttaggc ggtggttctc
     4861 cacacgactg cgcaaaaggt attgcgctgg tggcagccaa tggcggcgat attcgcgatt
     4921 acgaaggcgt tgaccgctct gcaaaaccgc agctgccgat gatcgccatc aataccacgg
     4981 cgggtacggc ctctgaaatg acccgtttct gcatcatcac tgacgaagcg cgtcatatca
     5041 aaatggcgat tgttgataaa catgtcactc cgctgctttc tgtcaatgac tcctctctga
     5101 tgattggtat gccgaagtca ctgaccgccg caacgggtat ggatgcctta acgcacgcta
     5161 tcgaagcgta tgtttctatt gccgccacgc cgatcactga cgcttgtgca ctgaaagccg
     5221 tgaccatgat tgccgaaaac ctgccgttag ccgttgaaga tggcagtaat gcgaaagcgc
     5281 gtgaagcaat ggcttatgcc cagttcctcg ccggtatggc gttcaataat gcttctctgg
     5341 gttatgttca tgcgatggcg caccagctgg gcggtttcta caacctgcca cacggtgtat
     5401 gtaacgccgt tttgctgccg catgttcagg tattcaacag caaagtcgcc gccgcacgtc
     5461 tgcgtgactg tgccgctgca atgggcgtga acgtgacagg taaaaacgat gcggaaggtg
     5521 ctgaagcctg cattaacgcc atccgtgaac tggcgaagaa agtggatatc ccggcaggcc
     5581 tacgcgacct gaacgtgaaa gaagaagatt tcgcggttct ggcgactaat gccctgaaag
     5641 atgcctgtgg ttttactaac ccgatccagg caactcacga agaaattgtg gcgatttatc
     5701 gcgcagcgat gtaatcatca tttccacaac ggctggcaaa ttgtcagccg ctttttcaac
     5761 tatctctgta acccttgccc gtaaattcgt gatagctgtc gtaaagctgt taccgactgg
     5821 cgaagatttc gccagtcacg tctacccttg ttatacctca caccgcaagg agacgatcat
     5881 gaccaataat cccccttcag cacagattaa gcccggcgag tatggtttcc ccctcaagtt
     5941 aaaagcccgc tatgacaact ttattggcgg cgaatgggta gcccctgccg acggcgagta
     6001 ttaccagaat ctgacgccgg tgaccgggca gctgctgtgc gaagtggcgt cttcgggcaa
     6061 acgagacatc gatctggcgc tggatgctgc gcacaaagtg aaagataaat gggcgcacac
     6121 ctcggtgcag gatcgcgcgg cgattctgtt taagattgcc gatcgaatgg aacaaaacct
     6181 tgagctgtta gcgacggctg aaacctggga taacggcaaa cccattcgcg aaaccagtgc
     6241 tgccgacgtg ccgctggcga ttgaccattt tcgctatttc gcctcgtgta ttcgggcgca
     6301 ggaaggtggg atcagtgaag ttgatagcga aaccgtggcc tatcatttcc atgaaccgtt
     6361 aggcgtggtg gggcagatta tcccgtggaa cttcccgctg ctgatggcga gctggaaaat
     6421 ggctcccgcg ctggcggcgg gcaactgtgt ggtgctgaaa cccgcacgtc ttaccccgct
     6481 ttctgtactg ctgctaatgg aaatcgtcgg tgatttactg ccgccgggcg tggtgaacgt
     6541 ggtcaacggc gcaggtgggg aaattggcga atatctggcg acctcgaaac gcatcgccaa
     6601 agtggcgttt accggctcaa cggaagtggg ccaacaaatt atgcaatacg ccacgcaaaa
     6661 cattattccg gtgacgctgg agctgggcgg caaatcgcca aatatcttct ttgctgatgt
     6721 gatggatgaa gaagatgcct ttttcgataa agcgctggaa ggctttgcac tgtttgcctt
     6781 taaccagggt gaagtttgca cctgtccgag tcgtgcctta gtgcaggagt caatctacga
     6841 acgctttatg gaacgcgcca tccgccgtgt cgaaagcatt cgcagcggta acccactcga
     6901 cagcgtgacg caaatgggcg cgcaggtttc tcacgggcaa ctggaaacca tcctcaacta
     6961 cattgatatc ggtaaaaaag agggcgcaga cgtgctcacc ggcgggcggc gcaagctgct
     7021 ggaaggtgaa ctgaaagacg gctactacct cgaaccgacg attctgtttg gtcagaacaa
     7081 tatgcgggtg ttccaggagg agatttttgg cccggtgctg gcggtgacca ccttcaaaac
     7141 gatggaagaa gcgctggagc tggcgaacga tacgcaatat ggcctgggcg cgggcgtctg
     7201 gagccgcaac ggtaatctgg cctataagat ggggcgcggc atacaggctg ggcgcgtgtg
     7261 gaccaactgc tatcacgctt acccggcaca tgctgcgttt ggtggctaca aacaatcagg
     7321 tatcggtcgc gaaacccaca agatgatgct ggagcattac cagcaaacca agtgcctgct
     7381 ggtgagctac tcggataaac cgttggggct gttctgatat aagaagctgg tcgcattggg
     7441 tattcattgc ctgatgcgac gcttacgcgt cttatcatgc ctacgggaac ctgaccgtag
     7501 gccggataag gcgtacacgc cgcatccggc ataaacaaag cgcacgttgt tacaatttac
     7561 gttttaaacc tacgtgcgca ataacccaga ctacgccaac agaagtgcgt ttctggggcg
     7621 tgttttgcca ggagtttact ctcgcgtttc gatggtgagc gggcaaacca ggccgtaaat
     7681 caccattggc gttccgaggc caatcagcaa taaacggtag ataattgaag gtttaatacg
     7741 ctttatctgt actgtggatg ttttactgga gttattcatt agcttggtca atagttccat
     7801 tatttcaata tgataaacct cgacctgttg aaaagccacg cagcacactt cagaaagtat
     7861 ataaaaaaca atatgccgtt atataaataa ggaaattgca ctgcatcatg ggatttcata
     7921 atatgcgtta attgttcttt tttatattct ctgagggaaa tatgtttctg gattattttg
     7981 cactgggagt gcttattttt gtatttctgg tgatattcta tgggattatt attttacatg
     8041 atattcccta cctgattgct aaaaaacgta atcatcctca tgccgacgcc attcatgttg
     8101 ctggttgggt gagtcttttt acgctgcatg ttatctggcc atttttgtgg atttgggcca
     8161 cgctttaccg cccggagcgg ggatggggaa tgcaaaacca tgactcctcc gttgtgcaac
     8221 tacaacagcg tattgccggg ctggagaaac agctcgccga catcaaatcc tcttctgccg
     8281 agtaatactt atggatttac tgattatttt gacctatgtg gcttttgcat gggcaatgtt
     8341 taagatcttc aaaattcctg taaataaatg gaccattccc acagcggccc tgggtggcat
     8401 atttattgtc agtggtttaa ttctgttaat gaactataac catccgtata ccttcaaagc
     8461 gcaaaaagcg gttatttcta ttcctgttgt cccacaggtg acaggcgtgg tgatcgaagt
     8521 gacggataag aaaaatacgc tgattaaaaa aggtgaggtg ctatttcgac tggacccgac
     8581 gcgttatcag gcgcgggtgg atcggctgat ggcggatatc gttaccgcag aacataaaca
     8641 gcgggcgttg ggcgcagagt tagatgagat ggcggcgaat actcagcagg caaaggccac
     8701 gcgggataaa ttcgctaaag agtataagcg ttacgcacgt ggcagccagg cgaaagtaaa
     8761 cccgttttca gaacgcgata tcgatgtggc gcggcaaaat tatctggcgc aggaagcctc
     8821 cgtgaagtca tcggcggcgg aacaaaaaca gatccagagc cagctggata gcctggtgtt
     8881 gggtgaacat tctcaaatcg ccagcctgaa agcacagctc gcggaagcaa aatataacct
     8941 tgagcagacg atagtgcgtg cgccgagcga tggttatgtg acccaggtac tgattcgtcc
     9001 gggtacctat gccgcgtcgc tgccgctacg tccggtgatg gtgtttatac ccgatcagaa
     9061 acgacaaatc gtggcgcagt tccgtcagaa ctccttgctg cgactggctc ctggcgacga
     9121 tgcggaagtg gtgtttaatg ctctgccagg taaggtattc agcggtaagc tggcagccat
     9181 tagtccagcc gttcccggcg gagcttatca gtcgaccggc accttacaga cgttaaacac
     9241 agcgccgggt tcagatggcg ttatcgcgac cattgaactg gatgagcaca ctgatttgag
     9301 cgctttacca gacggtattt acgcccaggt ggcggtttac tctgatcatt tcagccatgt
     9361 ctcggtgatg cgcaaagtgc tattacgcat gaccagctgg gtgcattacc tttatctcga
     9421 tcattaatct tcgctttccg ccgcttgttc aggccgcagt tgctgcgact gagcaagctg
     9481 gtggcgaatt tcacgcagca tttcatccag cacatactgg acccgccagg gctggtattc
     9541 ccgtttacgg aagatagcca ccagatccag ccaccattca tagggttgct gaaagcaggg
     9601 caccagcgtg ccgtcttgca ggtcgctctg cacacttttg tctggcgcaa acacaatccc
     9661 caggtgattt cgtgccagct ccagcgcaga ttgcgtgttg tcgcaaacgt aatttccctt
     9721 cacgcgataa tcccgcacct ctttactccc ggcgacgttg aagcgccaga tattcgcgtc
     9781 gtcgatcatc atcgagtcga tcaaaataca ggagtgatgt tcgagttcgt caggacggct
     9841 aatgggatgt ttctcaagat agcgctggct ggcataagcg gttacggcat attgggtaat
     9901 aaaactggca accagcgatt catcttttgg tggagcgtag ctgattaaaa catcacaatc
     9961 atcaggaaat tcgacgcctt cataaaaggc attacgctca agattgcagg tttttagcga
    10021 taaggtgata tcaccgatat ctttaatttt atcgattaca tgtttggata aataggtaat
    10081 tatgccggtt ggggcgtaga tggtgacccg accacgtttc tcatgcttat aatctgcaat
    10141 aaaattatta agttgctcgt ttctgtccag catgtcgttg atgtacggta acagcgcggt
    10201 gccaaaaggt gtgagcatca gctgccgggt ggttcggtca aaaactttta aacccacttt
    10261 tgattcaaaa tcagcaagat atttgctgac gttggcctgt gcgatgccaa gtacagtcgc
    10321 ggcatggctg atattttcac tggcagcgat taccgagata atttttaact cccggtactt
    10381 aagttgtaat tttgtcatcg caa
//
LOCUS       DAZFJF010000145        10384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFJF010000145 DAZFJF010000000
VERSION     DAZFJF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.146"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(163..2196)
                     /gene="betT"
                     /locus_tag="ACHKAU_004203"
     CDS             complement(163..2196)
                     /gene="betT"
                     /locus_tag="ACHKAU_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO5533157.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2325..2912
                     /gene="betI"
                     /locus_tag="ACHKAU_004204"
     CDS             2325..2912
                     /gene="betI"
                     /locus_tag="ACHKAU_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO5533158.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2926..4398
                     /gene="betB"
                     /locus_tag="ACHKAU_004205"
     CDS             2926..4398
                     /gene="betB"
                     /locus_tag="ACHKAU_004205"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO5533159.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4412..6082
                     /gene="betA"
                     /locus_tag="ACHKAU_004206"
     CDS             4412..6082
                     /gene="betA"
                     /locus_tag="ACHKAU_004206"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO5533160.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6295..6960
                     /gene="ykgH"
                     /locus_tag="ACHKAU_004207"
     CDS             6295..6960
                     /gene="ykgH"
                     /locus_tag="ACHKAU_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO5533161.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRVILFIIAICMAMMLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(7206..7901)
                     /locus_tag="ACHKAU_004208"
     CDS             complement(7206..7901)
                     /locus_tag="ACHKAU_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5533162.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7894..9321)
                     /locus_tag="ACHKAU_004209"
     CDS             complement(7894..9321)
                     /locus_tag="ACHKAU_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5533163.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9332..10051)
                     /locus_tag="ACHKAU_004210"
     CDS             complement(9332..10051)
                     /locus_tag="ACHKAU_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5533164.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2632 a         2798 c         2579 g         2375 t
ORIGIN      
        1 aaaataaaat cgttaattaa taaaatataa aatcatgaag tgatacaaga ggaattgcag
       61 gatatgacat gacaatcata tataatatat gcataatttt ttacagcgag tcaatgattc
      121 ccgcgccata tgcagacatg gcgcggtttt atgcaaaaac aatcacgcgt ccgggaacat
      181 cacgctatgg cccggcgctt cacgatggag atgaataaag ttaaggtgcc gctcgtactg
      241 gtcaagaata tcggtgatca cctgctcttt gctgtagtcc atcaggtcgt tgccctggct
      301 gccttctaac aggaaggttt ccagccggta gtaggtcgat ttaccgctac gtgcgcggta
      361 ggtaaagccc ggcaccgaat attgctgcgg ccaaatctga tagacaaagt tttgttcttc
      421 gcccatatgc accaacaaat ccagatgccc caactgttgt ccctcttccg gcggcaggct
      481 ttttagctcc acgtatgcgc cgcgcaaccg cagctcctgc gccacttctt ccattgccgg
      541 gtaacagacc gtctccatca tctgtttagt gtaacgcgtg cccggataat tcatcaggcg
      601 cgagagacgt tttttccagc tcaggcgatc ctgaagcccc agcggtcgcg gtgcggtatc
      661 gcggttggca ctttcacggc ggtaatcttc taccttcaga gatttataca accccgccat
      721 cacgaagaag atcacaaagc tgaacggcag ccccataatc accgtggtgt tttgcagcgc
      781 ggatatcccg ttggtcatca gcatgccgag cgtcagcagg ccaatcgcca ccgaccagaa
      841 gacgcgcagc cagccggggg cgtcgctgtt gatatcttta agctgcgagg tgaaattccc
      901 cagcaccagc gcaccggagt ccgccgaggt cacataaaac agcaggccag taatggtggc
      961 gacggaggcg ctaaaggtaa acgccggata ctgcgccagc aggctgtaga agccgcgctc
     1021 cggatggacc atcgcttcct cggcaaatgc cgcgccgccg tggatgattt catacagcgc
     1081 gctattgccg aacaccgaga gccataacag cgtgaaggta aacggaataa tcaacgtgcc
     1141 cagcacgaac tggcgaatgg tacgcccacg cgagatacgc gccaggaaca agccgacaaa
     1201 cggcgaccat gccacccacc atgcccagaa gaagagcgtc cagttattca tccactcaac
     1261 tggacggtcg aaggcaaaac tgttgagcgt catgcccata aagcgattca catagtcgcc
     1321 aacattcagc accagcgcat taagcaggaa cgaggtgtcg cccataaaca atacgaacag
     1381 gatcaatccc agcgccagcg caacgttaag ttccgataac acacgaatcc ccttatcgac
     1441 accggaggtg acagagatcg tggcgattat caccgacaag gcgatcagtg ccgcttttgc
     1501 cgccatcgaa tcgggaatat caaacagtac gctcaagcca tagttaagct gcaccacacc
     1561 gataccgagc gtagtggcaa taccgaagat ggtgccgatc accgctgcaa tatccactga
     1621 gtgccctatc ggcccgttaa tccgtttacc gaagatcggg tacagcgccg agcggatggt
     1681 gagcggcaaa ttataacgat agctaaagta tccgagcgcc atgcccatca gcgcatacat
     1741 cgaccagccg gttaagccgt agtgaaacag cgtccagacc atcgcctgac gcgcggcctc
     1801 aatcgtctgt cccgcgcctt ccggcggctg catatactgc gttaccggtt cggctacgga
     1861 gaagaacatc aggtcgatac cgatcccggc agcaaacagc atcgccgccc aactcagcag
     1921 gctgaattcc ggtttggatt gttctggccc gagcttcacc gaaccaaaac gcgaacaagc
     1981 gatacagacc acaaagacaa tatagagcgt tgccgccagt agatagtacc aaccgaaggt
     2041 tttagaaacc cagtccagcg tgcggccaat ccacagggcc gagaagtcgc gaaacaggat
     2101 cgttgtcagg gaaaacaaca aaatcagtcc ggcggaggtg taaaacacca ccggattgat
     2161 tttgtccttt tccctgctgt gtgaaaggtc tgtcatccag tatccccact gttattgtta
     2221 ccatttaaaa tcaaattcgt aacaattaag acacatttta tattgaacgt ccaatcaata
     2281 accgctttaa tagataaaca acgctgatga atggagtggc gaaaatgccc aaattgggga
     2341 tgcagtcgat ccggcgcaga caactgatcg acgccacact ggaagcaata aatgaagtgg
     2401 gcatgcacga tgcaacgatc gcgcagatcg cccgccgtgc aggcgtttct acggggatca
     2461 tcagccacta tttcagggac aaaaatggtc tgctggaagc aaccatgcgc gatatcacca
     2521 gtcagctgcg tgacgcggtt ttgaatcgat tacatgcact tccgcagggc agtgcagagc
     2581 agcgattaca ggcgattgtt ggcggaaact tcgatgaaac gcaggtgagc agtgcggcga
     2641 tgaaagcctg gctggcgttc tgggccagca gtatgcatca gccgatgctc tatcgtttac
     2701 agcaggtcag tagccgccgc ttattgtcga atctggtgag cgaatttcgc cgcgagttac
     2761 cgcgccaaca ggcacaggaa gcgggctacg gccttgccgc gctgattgac ggattatggc
     2821 tgcgcgcggc actgagcggc aaaccgctgg ataaacctct cgcacactcg ctgacccgtc
     2881 actttattac tcagcattta cccaccgatt aaccgaggag acgtgatgtc ccgaatggca
     2941 gaacagcagc tttatataca tggtggttat acctccgcca ccagcggtcg caccttcgag
     3001 accattaacc cggccaacgg taacgtgctg gcgaccgtgc aggccgccgg gcgtgaggat
     3061 gtcgatcgcg ccgtgaagag tgcccaacag gggcaaaaaa tctgggcggc gatgaccgct
     3121 atggagcgct cgcgtattct gcgtcgggcc gttgatattc tgcgtgaacg caatgacgaa
     3181 ctcgcaaaac tggaaaccct cgataccgga aaagcatatt cggaaacctc aaccgtcgat
     3241 atcgttaccg gtgcggacgt gctggagtac tacgccgggc tgatcccggc gctggaaggc
     3301 agccagatcc cgttgcgtga gacgtcattt gtttataccc gccgcgaacc gctgggtgtg
     3361 gtggcgggga ttggcgcatg gaactacccg atccagattg ccctgtggaa atccgccccg
     3421 gcgctggcgg caggcaacgc aatgattttc aaaccgagcg aagtcacccc gcttaccgcg
     3481 ttaaagctgg ctgaaattta cagcgaagcg ggcctgccgg acggcgtatt taacgtgttg
     3541 ccgggggggg gcgcggagac cgggcaatat ctgaccgagc atccgggcat tgccaaagtg
     3601 tcatttaccg gcggtgtcgc cagcggcaaa aaagtgatgg ctaactcggc ggcctcttcc
     3661 ctgaaagaag tgaccatgga actgggcggt aaatcaccgc tgatcgtttt cgacgatgcg
     3721 gatctcgatc tcgccgccga tatcgccatg atggcgaact tcttcagctc cggtcaggtg
     3781 tgtaccaacg gcacccgcgt cttcgttccg gcgaaatgca aagccgcatt tgagcagaaa
     3841 attctggcgc gcgttgagcg cattcgcgcg ggcgacgttt tcgatccgca aactaacttc
     3901 agcccgctgg tcagcttccc gcatcgcgat aacgtgctgc gctatatcgc caaaggcaaa
     3961 gaggaaggcg cgcgcgtact gtgcggcggc gatgtactga aaggcgatgg cttcgataac
     4021 ggcgcatggg ttgcaccgac cgtgttcacc gattgccgcg acgaaatgac catcgtgcgc
     4081 gaagagatct tcggtccggt gatgtcgctt ctcacttatg aatctgagga cgaagtgatt
     4141 cgccgcgcca atgataccga ctacggcctg gcggcgggca tcgtgacggc ggacctgaac
     4201 cgcgcgcatc gcgtcattca tcagctggaa gcgggtattt gctggatcaa cacctggggc
     4261 gaatccccgg cagagatgcc cgttggcggc tacaaacact ccggcattgg tcgcgagaac
     4321 ggcgtgatga cgctccagag ttacacccag gtgaagtcca tccaggttga gatggctaaa
     4381 ttccagtcca tattctaacc aggaggttta tttgcaattt gactacatca ttattggtgc
     4441 cggctcagcc ggcaacgttc tcgctacccg tctgactgaa gatccgaata cctccgtgct
     4501 gctgcttgaa gcgggcggcc cggactatcg ctttgacttc cgcacccaga tgcccgcagc
     4561 gctggcgttc ccactacagg gcaaacgcta caactgggcc tatgaaacag agcctgaacc
     4621 gtttatgaat aaccgccgca tggagtgcgg acgcggtaaa ggcctgggtg gatcgtcgct
     4681 gatcaacggc atgtgctaca tccgtggcaa tgcgctggat ctcgataact gggcgcaaga
     4741 acccggtctg gagaactgga gttatctcga ctgcctgccc tactaccgca aggccgagac
     4801 tcgcgatgtg ggcgagaacg actaccacgg tggcgatggc ccggtgagcg tcactacctc
     4861 caaacccggc gtcaatccgc tgtttgaagc gatgattgaa gcgggcgtgc aggcgggcta
     4921 cccgcgcacg gacgatctca acggttatca gcaggaaggt tttggtccga tggatcgcac
     4981 cgtcacgccg cagggccgtc gcgccagtac cgcgcgcggt tatctcgatc aggccaaatc
     5041 gcgccctaac ctgaccattc gtattcacgc tatgaccgat cacatcattt ttgacggcaa
     5101 acgcgcggtg ggcgtcgaat ggctggaagg cgacagcacc atcccaaccc gcgcaacggc
     5161 caacaaagaa gtgctgttat gtgcaggcgc gattgcctca ccgcagatcc tgcaacgctc
     5221 cggcgtcggc aacgctgaac tgctggcgga gtttgatatt ccgctggtgc atgaattacc
     5281 cggcgtcggc gaaaatcttc aggatcatct ggagatgtat ctgcaatatg agtgcaaaga
     5341 accggtttcc ctctaccctg ccctgcagtg gtggaaccag ccgaaaatcg gtgcggagtg
     5401 gctgtttggc ggcactggcg ttggtgccag caaccacttt gaagcaggtg gatttattcg
     5461 cagccgtgag gaatttgcgt ggccgaatat tcagtaccat ttcctgccag tagcgattaa
     5521 ctataacggc tcgaatgcag tgaaagagca cggtttccag tgccacgtcg gctcaatgcg
     5581 ctcgccaagc cgtgggcatg tgcggattaa atcccgcgac ccgcaccagc atccggcgat
     5641 tctgtttaac tacatgtcgc acgagcagga ctggcaggag ttccgcgacg caattcgcat
     5701 cacccgcgag atcatgcatc aacccgcgct ggatcagtat cgtggccgcg aaatcagccc
     5761 cggcacggaa tgtcagacgg atgaacagct cgatgagttc gtgcgtaacc acgccgaaac
     5821 cgccttccat ccgtgcggta cctgcaaaat gggttacgac gagatgtccg tggttgacgg
     5881 cgaaggccgc gtacacgggt tagaaggcct gcgtgtggtg gatgcgtcga ttatgccgca
     5941 gattatcacc gggaatttga acgccacgac aattatgatt ggcgagaaaa tagcggatat
     6001 gattcgtgga caggaagcgc tgccgaggag cacggcggga tattttgtgg caaatgggat
     6061 gccggtgaga gcgaaaaaat gagtcgtgat gtgaactaac gcaggaaccg atcagattga
     6121 gagttaccgt tccagagagg gggaccgaat ccttatataa acaccgaggt aactctcatg
     6181 cttcatatcc agtatccatg atgctggctt tttgctgata tttaacccga acattcctga
     6241 tacagactat taatacaacc ttatctgcga cagattttat tttctggata tattatgcgt
     6301 gaacaaatca aacaggatat cgatctgatt gagattttat tttatctgaa gaaaaaggtt
     6361 cgtgttatcc tttttattat agctatctgt atggcaatga tgcttttatt tctgtatatc
     6421 aataaagata atataaaagt gagttacagc ctaaaaataa accagacaac accaggcata
     6481 cttgttaact gtgatagcaa taataacttt gcctgtcaga ccacaatgac tgaagatgtt
     6541 atacagcgaa ttactacatt ttttcacacc agcccagatg tcaagaacag agaaataaag
     6601 ctggaatggt caggagataa gagagattta ccaactgctg aagcggaaat atctcgcgtg
     6661 caggcgtcta ttatcaaatg gtatgcgtca gaataccata atggcaggca agttctcgat
     6721 gagatacaaa caccttcagc aattaacagt gagctttata caaaaatgat atacctgacc
     6781 aggaactggt cattgtatcc gaacggtgat ggctgtgtaa ctataagctc accagaaata
     6841 aaaaataaat accctgctgc catttgcctg gctctgggat tttttctaag cattgtaatt
     6901 tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat accaacaaaa ctctgggtag
     6961 taataaacag cattctgata gagagtgcct gtataaagct gaagtgacaa tgaccgggaa
     7021 gggaacccgt tttcaggaat ggatgcctgt ctttttcatt gtaaatatcc tggcattact
     7081 aatgccagga tatttacaga agagcagcat aaccatgacg gaacggggtt aagtcaggtt
     7141 gtccttaacg ctattttcaa cctcgtatgc cttcttcagg tttatgttca gatctcatat
     7201 ctctctcagc aatcctcaat aatcagatac accgctttca ccggaccatg aaccccgacg
     7261 actttgataa gctcaatatc cgccgttgaa ctagggccgc taatgatgtt aatgcaggac
     7321 ggcattcgtt caccggcctg cgctttctga tgcaattttt ctgcgagttg cgctacacgc
     7381 ggcaagatag tgcttttacg caggataaaa agagaagatt ccgggagcag gctcaatgaa
     7441 cgcccgcgcc cggcggcgga aaaaagaacc acgcctcccg attcggttaa accatattca
     7501 gcatacacga cacccacttt agcctgctcg gcctgcgaga tattctcggc acctttcgcc
     7561 ggatcccaaa caacggcatt gcattcctgc tgcaaacgtt cactaatccc caattcctcc
     7621 agcctcgtgt caccgctaat cacgaccgac tgatctccca gctctttaca cagacgtatt
     7681 gcagcttctg ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat aacatcgctg
     7741 gcaaactgaa taaacgcgtc acagcgctgc tgttggttaa gttgggtaag ccgctcgtta
     7801 gcatagttgt taagcggcgc atcttctgct tgcggttcaa gtcgcagcgg gcgacccagt
     7861 gcctgagcaa cgttattcaa aaattcgcct cgattatcca ttctttttct cctgcgcctg
     7921 atgtttctta aaccaactac ggaaactctc tccgtcagct tcaggaagat cgcgtgcttc
     7981 catccagtcg ctaatcgcgc caaatttgag tggtgttttg ccgccattga taaaccagct
     8041 tgccgcatga gcgccggcca tcatcccgac tttccacaat cctggatgac tattggcata
     8101 agcgaacatt tttatcgccc gttgctctgc ttttgcggtg ataccttttt cagccatcac
     8161 ccgacgatga cgcaaaatca gttttgacag cggaatacgc accggacaca cgctgtcaca
     8221 agcagtgcat aaagagcagg cgtagggtaa atctttaaaa tctttatagc cgccaagtag
     8281 cggagaaatc accgcaccaa ttggccctgg ataaatagag ccatatccat gaccgccaat
     8341 atggcgatat gccggacaag tattcataca agccccgcag cgaatacagc gcagcacatc
     8401 ccgaaattca gaggccagca cctcagaacg cccgttatcg acaataacca gatgaaactc
     8461 ttcaggacca tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt
     8521 caaacgtgca ccaacggcac tgcgcgccag catggtgatc aatacatcta cctcggcaaa
     8581 cgtgggggca atacgctcca ttcccatcac tgcaatatgc gttttaggca gcgtggtaca
     8641 cattcgcgca ttaccttcat tggtcaccag gcataccgaa cctgtctctg ccaccgcgaa
     8701 attacagccg gtaataccta tttcagcact gaggaaatct tcgcggattt tttgccggat
     8761 gaataaggtc atcgcttcag gcgtttccgg cccctcatag cccagacgtt cgtgtagcac
     8821 tcgacggatc tgatggcgat ctttatgaat tgccgggacc acaacatgag atggcggatc
     8881 ttgatccagc tggagaatgt attcacccag atcggtttca atcacctgaa tgccagcatc
     8941 ctgcaacaca tgattgacac caatctcttc ggtcaccatc gatttagatt tcaccacctt
     9001 ccgggcattt ttgcgttggg caacctgtaa aatgtagcga gtggcgtctt ctttagtttt
     9061 tgcaaaatag acgtgaccgc cgttttgcgt cactttttct gagagctggt acagataagc
     9121 gtcgagatta cttagaacat gatcacgtat ctgggcggcc cgatcgcgcc actcctccca
     9181 gtgccccaat tcatcgacca ttttttgccg atttgcccca atacgctgct gcgcgtttgc
     9241 caccgctttg cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt
     9301 attactggtt ttgatcgaca tctttatatc ctcagcggct catcaacact tcagcaatat
     9361 gcatcacttt gactttctgc ccttcccgtt gtaatcgccc actgatgttc agcaggcaac
     9421 tcacgtcagc accaattaaa tactcagggc ggacttccat caggtgcaca accttttctt
     9481 tcaccatctc gccggatatt tcggccattt tgaccgagaa cgtgccgcca aatccgcagc
     9541 aggtatcctg ttcagcaaag gtcaacagct ccagtccacg cacatttttc agcagcgtaa
     9601 gtggctcgtc cttcactccc agcttacggg ccaggctaca agatgggtga tacacagctc
     9661 tcccttgcaa actggcacct acatcgacta cccctaattt attaacaata aaagaggtga
     9721 gatcctgcat acgcgcggca accttttcgg cacgtgatgc ccattcaggt tcatccgcca
     9781 gatacgtcgg gtaacttttt acggcatagg tgcaagagcc agccggtgaa ataatgggat
     9841 cgtcgttatc ctccagtgcg gcgatcagat ttttcatccc tggaatcgct tctttgatat
     9901 aaccgctatt gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa tttacgcgac
     9961 agccgagttt ttccagtagc agcacggagt ctcgtgccat tcttgatttc agggcgtcac
    10021 caatacaggt gacaaagaaa ttgacattca caactaatac tccattactt catgcccatt
    10081 tatgcccgat gtttcccccc caccggggga tcaatcatca ataaatatgg cgttcttttt
    10141 taattggcta gcaacatacc acccctcatt tgtatgacaa atgataatct ttcatccaaa
    10201 cttcaaatgc aataactaat tttatttctt tcggcatcgc tcacatttta tggggaaatc
    10261 gacgttcatt tttggcataa gaaatccatt taaaaaggct acatccctta tacctattca
    10321 gagctactta ttcaggggtt attgagatat acactgatgg tgtacaaaaa aatcaccgac
    10381 aata
//
LOCUS       DAZFJF010000146        10334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFJF010000146 DAZFJF010000000
VERSION     DAZFJF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.147"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..620
                     /gene="ssuE"
                     /locus_tag="ACHKAU_004211"
     CDS             45..620
                     /gene="ssuE"
                     /locus_tag="ACHKAU_004211"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO5533165.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            613..1572
                     /gene="ssuA"
                     /locus_tag="ACHKAU_004212"
     CDS             613..1572
                     /gene="ssuA"
                     /locus_tag="ACHKAU_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO5533166.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1569..2714
                     /gene="ssuD"
                     /locus_tag="ACHKAU_004213"
     CDS             1569..2714
                     /gene="ssuD"
                     /locus_tag="ACHKAU_004213"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO5533167.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGIDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2725..3516
                     /gene="ssuC"
                     /locus_tag="ACHKAU_004214"
     CDS             2725..3516
                     /gene="ssuC"
                     /locus_tag="ACHKAU_004214"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO5533168.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3513..4280
                     /gene="ssuB"
                     /locus_tag="ACHKAU_004215"
     CDS             3513..4280
                     /gene="ssuB"
                     /locus_tag="ACHKAU_004215"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5533169.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4487..7099)
                     /gene="pepN"
                     /locus_tag="ACHKAU_004216"
     CDS             complement(4487..7099)
                     /gene="pepN"
                     /locus_tag="ACHKAU_004216"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO5533170.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7365..8567
                     /gene="pncB"
                     /locus_tag="ACHKAU_004217"
     CDS             7365..8567
                     /gene="pncB"
                     /locus_tag="ACHKAU_004217"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO5533171.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8736..10136
                     /gene="asnS"
                     /locus_tag="ACHKAU_004218"
     CDS             8736..10136
                     /gene="asnS"
                     /locus_tag="ACHKAU_004218"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO5533172.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2330 a         2677 c         2785 g         2542 t
ORIGIN      
        1 atcaatgtta acaaaaaaag aacaattggt tataaggaga gagtatgcgt gtcatcaccc
       61 tggcgggtag tcctcgcttt ccttctcgct ccagctcctt gctggaatat gcgcgggaaa
      121 aactaaattg cctggatgta gaggtttatc actggaatct gcaaaacttc gccccggaag
      181 atctgcttta cgctcgtttc gatagtccgg cactcaagac cttcaccgaa cagctgcaac
      241 aggctgatgg actgatagtc gctacacctg tgtataaagc cgcctattcc ggcgcgctga
      301 aaaccctgct cgacctgctg ccagagcgcg ctttgcaagg caaagtggtg ctaccgctgg
      361 cgacgggcgg tacggtggct catctgctgg cggtcgatta tgcccttaag ccagttttaa
      421 gtgcgatgaa agcccaggag atcctgcacg gcgtgtttgc cgatgactca caagttattg
      481 attaccatca caaaccccag ttcacgccaa atctgcaaac ccgtcttgat accgcgctag
      541 aaactttctg gcaggcattg caccgccgcg atgttcaggt tcctgacctt ctgtctctgc
      601 gaggtaatgt ccatgcgtaa catcattaaa ctggcgctgg cgggattgct tagcgtctct
      661 acttttgcgg ttgctgcaga atcctcgcct gaagcgttac gtataggcta tcagaaaggc
      721 agtattggta tggtattggc aaaaagccac cagttactgg aaaaacgcta tccgcaatca
      781 aaaatctcct gggtggagtt ccccgcgggt ccgcaaatgt tggaagcgtt aaacgttggc
      841 agtattgatc tcggcagtac cggggatatt ccgccaatct ttgcccaggc tgccggggct
      901 gatttggtat atgtgggcgt cgagccaccg aagcccaaag ccgaagtgat tctggtggca
      961 gaaaacagcc cgatcaaaac cgtagccgat cttaaaggtc acaaagttgc ctttcagaaa
     1021 ggttccagtt cacacaacct tttactgcgt gcactgcgtc aggccggact taagtttacc
     1081 gatatccaac ccacttacct gacgcccgct gatgcccgcg ccgcgttcca gcaaggtaac
     1141 gttgacgcct gggctatctg ggatccctac tactctgctg cattattaca gggcgccgtg
     1201 cgggtgctga aagacggcac cgatctcaat caaactggat cgttttatct ggcagctcgt
     1261 ccctatgcag aaaaaaacgg cgcttttatt cagggcgtac tcgcaacctt tagtgaggcc
     1321 gatgcgttaa cccgcagcca gcgcgagcaa agcatcgctt tactggcaaa aacgatgggc
     1381 ttaccggcac cggtgattgc ctcttactta gatcatcgcc ctcctaccac catcaaaccg
     1441 gttaacgcca aggttgccgc cttacagcag caaacggcag atctgtttta tgaaaatcgt
     1501 ctggtgccga aaaaagtcga tattcgccag cgcatctggc agcccactca actggaagga
     1561 aaacaattat gagtctgaat atgttctggt ttttaccgac ccatggtgac gggcattatc
     1621 ttggaacgga agaaggttca cgcccggttg atcacggcta tctgcaacaa attgcgcaag
     1681 cggcggatcg tctgggctat accggtgtgc tgatcccaac ggggcgatcg tgtgaagatg
     1741 cgtggctggt ggcggcgtcg atgatcccgg tgacgcagcg gctgaagttt cttgtcgccc
     1801 tgcgccccag cgtaacctca cctaccgttg ccgctcgcca ggccgccacg cttgaccgtc
     1861 tctcaaatgg tcgtgcgttg tttaatctgg tcacaggcat cgatccacaa gagctggcag
     1921 gcgacggcgt gttccttgat catagcgagc gctacgaagc ctcggcggaa tttacccagg
     1981 tctggcggcg cttattgctg ggggaaaccg tcaatttcaa cggtaaacat attcatgtgc
     2041 gcggagcaaa actgctcttc ccgccgattc aacagcctta tcctccgctt tactttggcg
     2101 gatcgtcaga cgttgcccag gagctggcgg cagaacaagt tgatctctac ctcacctggg
     2161 gcgaaccgcc agaactagta aaagagaaga tcgaacaagt gcgggcgaaa gctgccgcgc
     2221 atggacgcaa aattcgtttc ggtattcgtc tgcatgtgat tgttcgtgaa actaacgacg
     2281 aagcgtggca ggccgccgag cggttaatct cgcatcttga tgatgaaact atcgccaaag
     2341 cacaggccgc attcgcccgg acggattccg taggacaaca gcgaatggcg gcgttacata
     2401 acggcaagcg cgacaatctg gagatcagcc ccaatttatg ggcgggcgtt ggcttagtgc
     2461 gcggcggtgc cggaacggcg ctggtgggcg atggtcctac ggtcgctgcg cgaatcaacg
     2521 aatacgccgc gcttggcatc gacagctttg tgctttcggg ctatccgcat ctggaagaag
     2581 cgtatcgggt cggcgagttg ctgttcccac atctggatgt cgccattccg gaaattcccc
     2641 agccgcagcc gctgaatccg caaggcgaag cggtggcgaa tgattttatc ccccgtaaag
     2701 tcgcgcaaag ctaaggaaga aataatggca acgccagtga agaagtggtt attgcgcgtt
     2761 gccccctggt ttttaccggt gggcatcgtg gcggtgtggc aactggcctc ctcagttggc
     2821 tggctgtcga cgcgtatttt gccctcaccg gaaggggtgg tgacggcgtt ctggacgctc
     2881 tccgccagcg gcgaactgtg gcagcatctg gcgatcagtt cctggcgggc gctgattggc
     2941 ttttcaattg gcggatcgct ggggttgatt ttgggactga ttagcgggct atctcgctgg
     3001 ggagaacggc tgctggatac ctcaattcag atgttgcgca acgtaccgca tctggcgctg
     3061 atcccgctgg tgattttgtg gtttggcatt gatgaatccg caaaaatctt tctggtggcg
     3121 ctcggcacgc tattccccat ttatatcaac acctggcatg gcatccgtaa tatcgatcgc
     3181 ggtctggtgg aaatggcgcg tagctatgga ttatccggca taccgctgtt tatccatgtg
     3241 atcctgcctg gtgccctgcc ctcaattatg gtcggcgtgc gttttgcgtt gggcctgatg
     3301 tggctgacgc tgattgttgc cgaaaccatt tctgccaatt caggcattgg ttatctggcg
     3361 atgaatgcgc gggagttttt gcaaacggac gtggtggtag tcgctattat tctttacgcc
     3421 ctgctcggca aacttgctga cgtcagcgcg cagctgttag agcgcctctg gttgcgctgg
     3481 aacccggctt atcatttgaa ggaggccacg gtatgaatac tgctcgtctg aaccagggca
     3541 caccattgtt gctcaatgca gtaagcaaac attacgcgga aaatatcgtc ctgaaccaac
     3601 tggatttaca tattccggca ggtcagtttg tggcggtggt gggccgcagc ggtggtggca
     3661 aaagtaccct gctgcgcctg ctggcaggtc tggaaacgcc aaccgcaggc gatgtgttag
     3721 cgggcaccac accgctggct gagattcaag aagacacgcg aatgatgttt caggatgcgc
     3781 gtctgctgcc gtggaaatcg gtgattgata acgttgggtt aggccttaaa ggccagtggc
     3841 gcgatgccgc gcgtcgggcg ttggctgcag taggactgga gaatcgcgcc ggggaatggc
     3901 ctgccgcact ttctggcggg cagaaacagc gtgtggcgct ggcaagagcg ttgattcatc
     3961 gaccgggttt attgttgctt gatgaaccgc tcggggcgct ggacgcctta acgcgactcg
     4021 agatgcagga tttgattgtg tcactttggc agcagcacgg ctttaccgta ctgttggtga
     4081 cgcatgatgt gagcgaagcg gtagcaatgg ctgaccgggt gttgttaatt gaagagggaa
     4141 aaattggtct ggatttgaca gtggatatcc cccgtccgcg tcgattaggg tcggtgcgat
     4201 tagcggagct ggaagcggaa gtattgcaac gggtaatgcg gcgtgggcat tctgaacaac
     4261 tgattcggcg tcatggttaa tatgccggat gtggcgcaca aaaccagcat tttcaacgga
     4321 ctagtaggcc tgataagaca cgtcaagcat cgcatcaggc agttgggcac ggctgccgga
     4381 tgcggcgtga acgccttatc cagcctacaa aaccagcaat ttcaacggag ttgtaggcct
     4441 gataagacgc gtcaagcgtc gcatcaggca ttttggctca aaagtatcaa gccagtgctt
     4501 tggtgatctt ctcgtacaga tcgccagaga gattttccag ccctttcaac tgttccagcg
     4561 ccgcgcgcat tttctcctga cgtttggcat cataacgttt caggcgaatc agcggttcaa
     4621 tcagacgtga agctacctgc gggttacggc tgttgaggtc ggtaagcatt tccaccagga
     4681 actggtaacc gctgccatct tcggcatgga acgctgccgg gttgctgccc gcaaacgcac
     4741 caatcaacga acgaatacgg ttcgggttgc tcatggtaaa tgagcgatgc tgcaacagac
     4801 cacgcaccgt ctccagcaca ttcgccgccg ggctggtggc ttgcaggata aaccatttat
     4861 ccatcaccag accgtcctga tgccacttat cgtcgtactc ctgcatcagc gcgtcacggc
     4921 aaggcagctg tgcagcaacc gccgcagaaa gcgctgccag cgcatcggtc atattgtttg
     4981 cttcatggta ttgcttgctc accagcacgt cagccagatg cgtttcacca aaagcaagga
     5041 agcgcaggca ggcattacgc agagtgcgtt ttgcaatatc ttcatgctca acacggtact
     5101 cgctctggta attcgcgttg taaatagcca gcagctcatc cgccagttca gtcgccagag
     5161 tacgagtgag tgcttcgcgt acttcggcaa tagcaatcgg gtcgatgata tcgaacagtt
     5221 cagccatttc attgacagaa ggcagcgtca ggatttctgc cgccagcgcc ggatcaatct
     5281 tctcatcgag cagcaccgcg cggaaagcgt cagccacatg taccggcaga gacagcggct
     5341 gcccttgctg atgacgggcg acgttcagct tgatgtaggt tgccagcaaa ctttgcgccg
     5401 catcccaacg ggagaaatca ttacgcgcat gacgcatcag gaaggtcagt tgctgatcgc
     5461 tccacttata ttccagtttc actggcgcag agaattcgca cagcagcgca ggcaccggct
     5521 ggaagtagac attatcaaaa acaaaggtct gttccgcctg ggtgacgttc agcacggaat
     5581 tcaccggatg accgcctttc tgcaacggga tcactttgcc ttcgttgtca tacaattcga
     5641 tggcaaatgg aatatgcagc ggctgttttt ctgcctgatc cggcgtggct ggcgtgcgct
     5701 ggctgatggt cagggtgtac tgctcggttt ccggattgta gtcgtctttg acggtcacaa
     5761 tcggcgtgcc ggactggctg taccaacggc ggaaatggga gagatcgaca ttcgacgcat
     5821 cttccatcgc ctgcacaaag tcgtcgcagg ttgccgcgct gccatcatgg cgctcgaaat
     5881 agagttgcat ccctttctgg aagttttctt cgcccagcag ggtgtggatc atgcgaatca
     5941 cttccgcgcc cttctcgtaa acggtcaggg tgtagaagtt gttcatctca atgaccatat
     6001 ccgggcggat cgggtgcgcc atcgggctgg cgtcttccgc aaactgcaat ccgcgcatgg
     6061 tgcgcacatt attgatacgg tttaccgcgc gggaaccaag gtcagagctg aattcctgat
     6121 cgcggaagac ggttaaacct tctttcaggc tgagctggaa ccagtcgcgg caggtcactc
     6181 ggttaccggt ccagttatgg aaatattcat ggccgataac gcgttcaata tcgagataat
     6241 ctttgtcggt ggcggtgtcg gtgcgggcca acacatattt ggagttaaag atattcagac
     6301 ccttattctc cattgcgccc atattgaaga aatccaccgc gacgatcata tagatgtcga
     6361 ggtcatactc caggccaaag cgttcttcat cccatttcat ggagtttttc agcgaggtca
     6421 tcgcccacgg cgcgcgatca aggttgccgc gatcgacgta cagctccagt gctacttcgc
     6481 gaccagaacg cgtggtaaag gtgtcgcgca gtacatcaaa gtcgcctgcc accagcgcaa
     6541 acaggtagca cggtttcggg aacgggtcct gccactgtac ccaatggcgt ccgttttcca
     6601 gttcaccttg cgcaacgcgg ttaccgttgg aaagcaggaa gggatatttg gttttatcgg
     6661 caataatttt ggtggtaaaa cgcgccagca cgtccgggcg gtcgagataa tacgtaatat
     6721 ggcggaaacc ttcggcttca cactgggtgc aaagcgcatc gcctgactga taaagccctt
     6781 ccagcgccgt attcgccgcc gggcttattt cattaatgat cttaagcgta aaacgctccg
     6841 gcaaattact gatgaccagt gcgccctctt cttctttcca ggcggtccac ggctcatcat
     6901 taatatgaac agaaaccagt ttgaggtctt cgccgttgag acgaagcgga gcatctgatg
     6961 caccatgacg gacagcctgg ctgaccgcgg taacgaccgt cttttgcgcg tcgaggtcaa
     7021 aggtcaagtc aatatcagta atctggtaat ccggcgcacg ataatcgtga cggtatttgg
     7081 cttgtggctg ttgagtcata aataaccttt agcatctttt atagagtctg gtgttcagtc
     7141 tattcctgtt gcgtaaatcg cgctatgcag aatcttcatc ttttcaggta caaacgcctt
     7201 tattgctaca tttttataac atacaccgcg taatgccatc gaccagaaag gtggcatatg
     7261 gtgtgatcgg ggttcaataa attgcgaaac aaggtatact ccagcagttc ctgaagatgt
     7321 ttattgtact aaacgctcct gtacgaggac gctactgcgc acctatgaca caattcgctt
     7381 ctcctgttct gcactcgttg ctggatacag atgcttataa gttgcatatg cagcaagccg
     7441 tgtttcatca ctattacgat gtgcatgtcg cggcggagtt tcgttgccga ggtgacgatc
     7501 tgctgggtat ttatgccgat gctattcgtg aacagattca ggcgatgcag cacctgcgcc
     7561 tgcaggatga tgaatatcag tggctctctg ccctgccttt ctttaaggcc gactatctta
     7621 actggttacg cgagttccgc tttaacccgg aacaagtcac cgtatccaac gataatggca
     7681 agctggatat tcgtttaagc ggcccgtggc gtgaagtcat cctctgggaa gttcctttgc
     7741 tggcggttat cagtgaaatg gtacatcgct atcgctcacc gcaggccgac gttgcgcaag
     7801 ccctcgacac gctggaaagc aaattagtcg acttctcggc gttaaccgcc ggccttgata
     7861 tgtcgcgctt ccatcttatg gattttggca cccgtcgccg tttttctcgc gaagtacaag
     7921 aaaccatcgt taagcgtctg caacaggaat cctggttcgt gggcaccagc aactacgatc
     7981 tggcgcgtcg gctttccctc acgccgatgg gaacacaggc acacgaatgg ttccaggcac
     8041 atcagcaaat cagcccggat ctagccaaca gccagcgagc tgcacttgct gcctggctgg
     8101 aagaatatcc cgaccaactt ggcattgcat taaccgactg catcactatg gatgctttcc
     8161 tgcgtgattt cggtgtcgag ttcgccagtc gctatcaggg cctgcgtcat gactctggcg
     8221 acccggttga atggggtgaa aaagccattg cacattatga aaagctggga attgatccac
     8281 agagtaaaac tctggttttc tctgacaatc tggatttacg caaagcggtt gagctatacc
     8341 gccacttctc ttcccgcgtg caattaagtt ttggtattgg gactcgcctg acctgcgata
     8401 tcccccaggt aaaacccctg aatattgtga ttaagttggt tgagtgtaac ggtaaaccgg
     8461 tggcgaaact ttctgacagc cctggcaaaa ctatctgcca cgataaagcg tttgttcggg
     8521 cgctgcgcaa agcgttcgac cttccgcata ttaaaaaagc cagttaatat catcagggag
     8581 ctaatcggct cccttttttt acctttaatt ccgaaatctt tcgctgcatt tgcgaattct
     8641 gcttgtctga ttgcagatgc caggtaacat aggtatcccc ccatttacgg gtggacaagt
     8701 gtttattttt tccgactatt aacagagaga atattatgag cgttgtgcct gtagccgacg
     8761 tactccaggg ccgcgtagcc gttgacagcg aagtcaccgt acgcggatgg gtacgtaccc
     8821 gccgagattc aaaagctggc atctccttcc tcgccgttta tgacggttcc tgctttgatc
     8881 ctgtacaggc tgtcatcaat aattctctgc ccaattacaa tgaagacgtc ctgcgtctga
     8941 ccaccggctg ctcggtcatt gtgacgggta aagtcgtggc gtcgccgggc caggggcaac
     9001 aatttgaaat tcaggccagc aaggttgaag ttgctggttg ggttgaagat ccagacactt
     9061 acccgatggc ggcaaaacgc cacagcattg agtatctgcg tgaagtcgct cacctgcgtc
     9121 cgcgcacaaa cctgattggt gccgtcgcgc gcgttcgtca tacgctggcg caggcgctgc
     9181 atcgcttctt taacgagcag ggattcttct gggtttcaac gccactgatt accgcatctg
     9241 ataccgaagg tgcaggcgaa atgttccgcg tttctacgct ggatctggaa aacctgccgc
     9301 gtaacgatca gggcaaagtg gatttcgaca aagacttctt tggtaaagag tctttcctga
     9361 ccgtatctgg ccagttgaac ggcgaaacct acgcttgcgc attatccaaa atttatacct
     9421 tcggcccgac tttccgtgct gaaaactcca acaccagccg tcacctggcg gaattctgga
     9481 tgctggagcc ggaagtggcg tttgctaacc tgaacgatat cgcgggtctg gctgaagcca
     9541 tgctgaaata tgtcttcaaa gcggttctcg aagaacgcgc agacgacatg aaattcttcg
     9601 ctgaacgcgt agataaagat gccgtttcac gtctggaacg cttcattgag gccgatttcg
     9661 cgcaggtgga ttacaccgac gcagtgacca ttctcgaaaa ctgcggcagg aagtttgaaa
     9721 acccggttta ctggggcgtc gatctctctt ctgagcatga gcgttatctg gcggaagaac
     9781 actttaaagc accggtagtg gttaaaaact atccgaaaga tattaaagcg ttctatatgc
     9841 gcctcaacga agacggtaaa accgttgcgg ctatggacgt tctggctccg ggcatcggtg
     9901 agatcattgg tggctcccag cgtgaagaac gtctggacgt tctggacgag cgtatgctgg
     9961 aaatgggcct gaataaagaa gattactggt ggtatcgcga tctgcgtcgc tacggtactg
    10021 ttccgcattc aggtttcggt cttggttttg aacgcctgat tgcttacgta actggcgtgc
    10081 aaaacgtgcg tgatgtgatt ccgttcccac gtactccgcg caacgccagc ttctgatttt
    10141 cttcctcaaa gagccagcgc ctgctggctc ttttttatcc tttctgtcaa gttatctgtt
    10201 tgttaagtca agcaatctat ttgcaacccc gccataacga tcatcctgtt acggaatatt
    10261 acattgcaac atttacgtac aaaaaataac ccgcattctt attgcggatt agttttttct
    10321 tagctgatag caca
//
LOCUS       DAZFJF010000147        10200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFJF010000147 DAZFJF010000000
VERSION     DAZFJF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.148"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            98..1492
                     /gene="galP"
                     /locus_tag="ACHKAU_004219"
     CDS             98..1492
                     /gene="galP"
                     /locus_tag="ACHKAU_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO5533173.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1569..2066
                     /locus_tag="ACHKAU_004220"
     CDS             1569..2066
                     /locus_tag="ACHKAU_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO5533174.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2161..2868
                     /gene="endA"
                     /locus_tag="ACHKAU_004221"
     CDS             2161..2868
                     /gene="endA"
                     /locus_tag="ACHKAU_004221"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO5533175.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2948..3679
                     /gene="rsmE"
                     /locus_tag="ACHKAU_004222"
     CDS             2948..3679
                     /gene="rsmE"
                     /locus_tag="ACHKAU_004222"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO5533176.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3692..4642
                     /gene="gshB"
                     /locus_tag="ACHKAU_004223"
     CDS             3692..4642
                     /gene="gshB"
                     /locus_tag="ACHKAU_004223"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO5533177.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VAYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4751..5314
                     /locus_tag="ACHKAU_004224"
     CDS             4751..5314
                     /locus_tag="ACHKAU_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO5533178.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5314..5730
                     /gene="ruvX"
                     /locus_tag="ACHKAU_004225"
     CDS             5314..5730
                     /gene="ruvX"
                     /locus_tag="ACHKAU_004225"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO5533179.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5906..6886)
                     /locus_tag="ACHKAU_004226"
     CDS             complement(5906..6886)
                     /locus_tag="ACHKAU_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO5533180.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6904..7608
                     /gene="yggS"
                     /locus_tag="ACHKAU_004227"
     CDS             6904..7608
                     /gene="yggS"
                     /locus_tag="ACHKAU_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO5533181.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7626..8192
                     /gene="yggT"
                     /locus_tag="ACHKAU_004228"
     CDS             7626..8192
                     /gene="yggT"
                     /locus_tag="ACHKAU_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO5533182.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8189..8479
                     /gene="yggU"
                     /locus_tag="ACHKAU_004229"
     CDS             8189..8479
                     /gene="yggU"
                     /locus_tag="ACHKAU_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO5533183.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8487..9080
                     /locus_tag="ACHKAU_004230"
     CDS             8487..9080
                     /locus_tag="ACHKAU_004230"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO5533184.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            9073..>10200
                     /gene="hemW"
                     /locus_tag="ACHKAU_004231"
     CDS             9073..>10200
                     /gene="hemW"
                     /locus_tag="ACHKAU_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO5533185.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFL"
BASE COUNT         2386 a         2702 c         2707 g         2405 t
ORIGIN      
        1 tgtaagcgat tacactgatg tgatttgctt cacatctttt tacgtcgtac tcacctatct
       61 taattcacaa taaaaaataa ccatattgga gggcatcatg cctgacgcta aaaaacaggg
      121 gcggtcaaac aaggcaatga cttttttcgt ctgcttcctt gccgctctgg cgggattact
      181 ctttggcctg gatatcggtg taattgctgg cgcactgccg tttattgcag atgaattcca
      241 gattacttcg cacacgcaag aatgggtcgt aagctccatg atgttcggtg cggcagtcgg
      301 tgcggtgggc agcggctggc tctcctttaa actcgggcgc aaaaagagcc tgatgatcgg
      361 cgcaattttg tttgttgccg gttcgctgtt ctctgcggct gcgccaaacg ttgaagtact
      421 gattctttcc cgcgttctgc tggggctggc ggtgggtgtg gcctcttata ccgcaccact
      481 gtacctgtca gaaattgcgc cggaaaaaat tcgcggcagt atgatctcga tgtatcagtt
      541 gatgatcact atcgggatcc tcggtgctta tctttctgat accgccttca gctacaccgg
      601 tgcatggcgc tggatgctgg gtgtgattat catcccggca attttgctgc tgattggtgt
      661 cttcttcctg ccagacagcc cacgttggtt tgccgccaag cgccgttttg ttgatgccga
      721 acgcgtgctg ctacgcctgc gtgacaccag cgcggaagcg aaacgcgaac tggatgaaat
      781 ccgtgaaagt ttgcaggtta aacagagtgg ctgggcgctg tttaaagaga acagcaactt
      841 ccgccgcgcg gtgttccttg gcgtactgtt gcaggtaatg cagcaattca ccgggatgaa
      901 cgtcatcatg tattacgcgc cgaaaatctt cgaactggcg ggttatacca acactaccga
      961 gcaaatgtgg gggaccgtga ttgtcggcct gaccaacgta cttgccacct ttatcgcaat
     1021 cggccttgtt gaccgctggg gacgtaaacc aacgctaacg ctgggcttcc tggtgatggc
     1081 tgctggcatg ggcgtactcg gtacaatgat gcatatcggt attcactctc cgtcagcgca
     1141 gtatttcgcc atcgccatgc tgctgatgtt tattgtcggt tttgccatga gtgccggtcc
     1201 gctgatttgg gtactgtgct ccgaaattca gccgctgaaa ggccgcgatt ttggcatcac
     1261 ctgctccacc gccaccaact ggattgccaa catgatcgtt ggcgcaacgt tcctgaccat
     1321 gctcaacacg ctgggtaacg ccaacacctt ctgggtgtat gcggctctga acgtactgtt
     1381 tatcctgctg acattgtggc tggtaccgga aaccaaacac gtttcgctgg aacatattga
     1441 acgtaatctg atgaaaggtc gtaaactgcg cgaaatcggc gctcacgatt aatctcctca
     1501 ggcttcctcc catcgcggag gaagccacct cttgcagtca tcttttcttc gctctatcct
     1561 ctgccgctat gaaaacatcc cgtctcccta tcgccatcca gcaggccgtt atgcgccgcc
     1621 tgcgggaaaa actcgcccag gccaacctga agctagggcg taactacccg gagccaaaac
     1681 tctcttacac ccagcgcgga acctccgccg gaacagcctg gctggaaagc tatgaaatcc
     1741 gcctcaatcc cgttttgctg ttggaaaaca gtgaagcttt tattgaagaa gtggtaccgc
     1801 acgaactggc acatttgctg gtatggaaac atttcggccg cgtagcgcca catggcaaag
     1861 agtggaagtg gatgatggaa agcgtgctgg gcgttcccgc ccgtcgtacg catcagttcg
     1921 aactgcaatc cgtgcgtcgc aacaccttcc cctaccgctg caagtgccag gagcatcagc
     1981 ttaccgtacg ccgccataat cgcgtagttc gtggcgaggc cgtctatcgc tgtgttcact
     2041 gcggtgaaca gctggttgcg aaataaccat ctgaactatc aggaactttc ctgatctggc
     2101 tgattgcata ccaaaacaac tttcgctacg ttgctggctc gttttaacac ggagtaagtg
     2161 atgtaccgtt atttgtctat tgctgcggtg gtactgagcg cagcattttc cggcccggcg
     2221 ttggccgaag gtatcaatag tttttctcag gcgaaagccg cggcggtaaa agtccacgct
     2281 gacgcgcccg gtacgtttta ttgcggatgt aaaattaact ggcagggcaa aaaaggcgtt
     2341 gttgatctgc aatcgtgcgg ctatcaggtg cgcaaaaatg aaaaccgagc cagccgcgta
     2401 gagtgggaac acgtcgttcc cgcctggcag ttcggtcacc agcgccagtg ctggcaggac
     2461 ggtggacgta aaaactgcgc taaagatccg gtctatcgca agatggaaag cgatatgcat
     2521 aacctgcaac cgtcagtcgg cgaggtgaat ggcgatcgcg gcaactttat gtacagccag
     2581 tggaatggcg gcgaaggcca gtacggtcaa tgcgccatga aggtcgattt caaagaaaaa
     2641 gctgccgaac caccagcgcg tgcacgcggt gccattgcac gcacctactt ctatatgcgc
     2701 gaccaataca acctgacact ctctcgccag caaacgcagc tgttcaacgc atggaacaag
     2761 atgtatccgg ttaccgactg ggagtgcgag cgcgatgaac gcatcgcgaa ggtgcaaggc
     2821 aatcataatc cgtatgtgca acgcgcttgc caggcgcgaa agagctaacc tacactagcg
     2881 ggactctttt tgttaacccc taccccacgc gtacaaccgc gtggggagac gacgcggatt
     2941 tttaactatg cgtatccccc gcatttatca tcctgaacca ctgaccagcc attctcacat
     3001 cgcgctttgc gaagatgccg ccaaccatat cgggcgcgta ctgcgcatgg ggccggggca
     3061 ggcgttgcaa ttgtttgacg gtagcaacca ggtctttgac gccgaaatta ccagcgccag
     3121 caaaaaaagc gtggaagtga aggtgctgga aggcaagatc gacgatcgcg aatctccgct
     3181 gcatattcac ctcggtcagg tgatgtcgcg tggtgaaaaa atggaattta cgatccagaa
     3241 atcgatcgaa ctcggtgtaa gcctcattac gccacttttt tctgagcgct gcggcgttaa
     3301 actggatagt gaacgtctga acaagaagct ccagcagtgg cagaagattg caattgctgc
     3361 ctgtgagcag tgtggtcgta accgggtgcc ggaaatccgc ccagcgatgg atctggaagc
     3421 ctggtgtgca gagcaggatg aaggactgaa actgaatctt cacccgcgcg ccagtaacag
     3481 catcaatacg ttgccgttac cggttgaacg cgtccgcctg ctgattggcc cggaaggcgg
     3541 tttatcggca gatgaaattg ccatgactgc ccgctatcaa tttactgata tcctgttggg
     3601 acctcgcgtt ttgcgtacag agacaactgc gctcaccgcc attaccgcgc tacaagtacg
     3661 atttggcgat ttgggctaac ggagaagaat aatgatcaag ctcggcatcg tgatggaccc
     3721 catcgcaaac atcaacatca agaaagattc cagtttcgct atgttgctgg aagcacagcg
     3781 tcgtggttac gaacttcact atatggagat gggcgatctg tatctgatca atggcgaagc
     3841 ccgcgcccat acccgcacgc tgaacgtgaa gcagaactac gaagagtggt tttcgttcgt
     3901 cggtgaacag gatctgccgc tggccgatct cgatgtgatc ctgatgcgta aagacccgcc
     3961 gtttgatacc gagtttatct acgcgaccta tattctggaa cgtgccgaag agaaagggac
     4021 gctgatcgtt aacaagccgc agagcctgcg cgactgtaac gagaaactgt ttaccgcctg
     4081 gttctctgac ttaacgccag aaacgctggt tacgcgcaat aaagcgcagc taaaagcgtt
     4141 ctgggagaaa cacagcgaca tcattcttaa gccgctggac ggtatgggcg gcgcgtcgat
     4201 tttccgcgtg aaagaaggcg atccaaacct cggcgtgatt gccgaaaccc tgactgagca
     4261 tggcactcgc tactgcatgg cgcaaaatta cctgccagcc attaaagatg gcgacaaacg
     4321 cgtgctggtg gtggatggcg agccggtagc gtactgcctg gcgcgtattc cgcagggggg
     4381 cgaaacccgt ggcaatctgg ctgccggtgg tcgcggtgaa cctcgtccgc tgacggaaag
     4441 tgactggaaa atcgcccgtc agatcgggcc gacgttgaaa gaaaaagggc tgatttttgt
     4501 tggtctggat atcatcggcg accgtctgac tgaaattaac gtcaccagcc caacctgtat
     4561 tcgtgagatt gaagcagagt ttccggtgtc gatcaccgga atgttaatgg atgccatcga
     4621 agcacgttta cagcagcagt aacccacctt agcgagaagg atctcgttga gactctgagt
     4681 gacagcgccc ttctttccac gcatactggg cgctgttgct tttttgaacc aggaaacaga
     4741 acctctgaca atgaatttac agcatcactt tcttattgcc atgcctgctc tccaggatcc
     4801 gattttccgt cgttccgtgg tctacatttg cgaacataat accaatggtg caatggggat
     4861 catcgtcaac aagccgctgg aaaatctcaa aattgaaggg attctggaaa agctgaagat
     4921 cacgccggag ccgcgtgatg aatcaatccg tctggataaa ccggttatgc tcggcggtcc
     4981 gctggctgaa gatcgcgggt ttattttgca tactccgccc tccaattttg cttccagcat
     5041 tcgcatttca gacaacacag tgatgaccac ttcccgcgat gtgctggaaa cgctcggcac
     5101 cgataaacaa ccgtctgacg tattggtggc tctgggttat gcctcctggg aaaaaggtca
     5161 actggaacaa gaaattctcg ataacgcgtg gctaacggcc ccggcggatc tgaatattct
     5221 gttcaaaacg ccgattgccg accgctggcg cgaggcggca aaactgattg gtgtggatat
     5281 tctcaccatg cctggtgtgg cagggcacgc ctgatgagtg gaaccttact cgccttcgac
     5341 ttcggcacca aaagcattgg cgtagcggtc ggccaacgca ttaccggcac tgctcgccct
     5401 ttgcctgcaa ttaaagcaca ggacggtacg ccggactgga acctcatcga acgtttactg
     5461 aaagagtggc agccggacga aatcatcgtc ggtttgccgc tgaatatgga cggcaccgag
     5521 caaccattga ctgccagagc gcgtaaattt gccaaccgta ttcatggtcg tttcggtgtt
     5581 gaagtaaagc tccatgacga acgtcttagc accgtggaag cccgttccgg tctgtttgaa
     5641 cagggcggct atcgggcgct caacaaaggc aaagtcgact ctgcctctgc ggttattatt
     5701 ctcgaaagct atttcgagca gggatattaa ggcgatctaa aagcctggcg gatagtcgtt
     5761 accgccatct tgccggatgc ggcgtgaacg ccttatccag cacacatctg gcagcggctg
     5821 taggtctgat aagacgcgtt agcgtcgcat cagacattta ttgcctttat aggccctata
     5881 aacttgcgca tcgggcatgg caacgtcaca aacgcccttc ccccacccgc tgctgataac
     5941 tctgctgaaa cgttaacatc cccacctgct gcccggtttg aataacatgc ggtaactggt
     6001 gggttttccc ttcgcgaatc aaattcccca ccgcgggagt gttaatcaat aattcaaaca
     6061 gcgccacgcg tccttcctgt ttatccactt ccagcttttg tgacaacacg gcccgtaaac
     6121 tacctgccag ttgattacgt acgggatctt tttcctgcgc cggaaatgaa tccaccagtc
     6181 gctcaactgc ctgcgcggca ccacgcgtat gtaatgttgc cagcaccaaa tgcccggttt
     6241 ctgccgccgt cagtgccaga cggattgtct cgctgtcacg cagctctccg agcaaaatca
     6301 catcaggatc ttcccgcaat gcggcccgca atcccgatgc gaacgtcata cagtgcaaac
     6361 caatttcccg ctgctggatc aaacatcgct ggctggcata gatatattca acaggatctt
     6421 ccagcgtcag aatatgcgca tcggcatgtt gatagagata gccaaccatc gccgctagcg
     6481 tggtagattt gccgctcccc gtcgcccccg tcaccagaat caggccattc tcgctcttga
     6541 gtaattccgg caataccgtt ggtgcgccaa gctgttcgag ctgtggacag tgcgaaggta
     6601 acaaccgtaa tgccagagaa ataccttgcc gttgcgcgaa cgcactgcca cgcaaccgct
     6661 ggttttccgc cagcgacacg gcaaaatcca gctggccatt ttccagcaat attgcccgct
     6721 gatcgtcatc cagccactcc cgcagtagct cttcgacgtc cggcgcatca aacggcgcag
     6781 cttccattag cccgcgaatg cgccatcgtg cggaccaggc gctgcacagg tgtagatccg
     6841 agacgttatg ctttacacta agggccacaa tttcttccat attcatacta agatcctcgg
     6901 aaaatgaacg atattgcgca taacctggca caggtccggg acaaaatctc agcggctgca
     6961 acgcgttgcg gccgttctcc agaagaaatt acgctgcttg cagtcagtaa aacaaaacct
     7021 gcgagcgcca tcgcagaagc cattgatgcc gggcagcgtc aatttggtga aaactacgtt
     7081 caggaagggg tagataaaat tcgccacttt caggaactgg gcgtaacagg attagaatgg
     7141 cattttattg gcccgttgca gtctaataaa agccgcctgg tggcagagca tttcgactgg
     7201 tgtcatacca tcgaccgttt gcgcatcgct acccgcctca acgatcagcg cccggcagaa
     7261 cttccccctc ttaacgtact gattcaaatt aatattagtg atgaaaacag taagtccggg
     7321 attcaactgg cagaactgga cgagctggca gctgcggtcg ctgaactacc gcgtttacgt
     7381 ctgcgcgggc tgatggcaat ccctgcacct gagtcagaat atgtaaggca gtttgaagtt
     7441 gcacgccaaa tggctgtagc atttgccgga ctgaaaacgc gctacccgca tatcgacacg
     7501 ctctctctgg gaatgtcgga cgatatggaa gccgccattg cggcaggtag cacgatggtt
     7561 cgtatcggca ctgcaatttt tggtgcgcgt gattactcta aaaaataagg aattaaagga
     7621 acgccatgaa tacgttgact ttcctgcttt caacggtcat tgagctgtat accatggtgc
     7681 tgttattacg catctggatg cagtgggctc attgtgattt ttacaacccc ttctcacagt
     7741 ttgtagtgaa ggtaacgcag ccaattatcg ggccactgcg ccgcgttatt ccggcaatgg
     7801 gccccattga cagcgcctcg ctgctggttg cctatattct cagttttatc aaagccatcg
     7861 tgctgtttaa agtggtgacc ttcctgccaa tcatctggat tgccggttta ctgattctgc
     7921 tgaaaaccat cggcctgctg attttctggg tcctgctggt gatggcgatt atgagctggg
     7981 taagccaggg tcgtagcccg attgaatacg tgctgattca gctggccgat ccgctgctgc
     8041 gcccgattcg ccgcctgcta ccggcaatgg gtgggattga tttctcgccg atgatcctcg
     8101 ttctgctgct gtatgtcatc aatatgggtg tcgcagaagt attacaggca accggaaaca
     8161 tgctgctgcc ggggctgtgg atggcgttat gagtgccgta acagttaatg atgacggtct
     8221 ggttttacgg ctctatattc agccgaaagc cagccgtgat tctattgtcg gtttacatgg
     8281 cgacgaagtt aaagtcgcca ttaccgcgcc gccggttgac ggtcaggcca acagtcatct
     8341 ggtgaagttt ctcggtaagc aattccgggt tgccaaaagc caggtggtga ttgaaaaagg
     8401 cgaacttggc cgccacaaac aaattaaaat cattaatccg caacaaatcc cgccagaaat
     8461 cgcggcgtta attaattagg tatcctatgc aaaaagttgt cctcgcaacc ggcaatgccg
     8521 gtaaagtgcg tgagctggcg tcgctgctta gcgacttcgg tcttgatatc gtggcccaaa
     8581 cagacctcgg cgtagattcc gctgaagaaa ccggcctgac ctttatcgaa aacgcgattc
     8641 tgaaagcgcg ccatgcggca aaagtgaccg gtttaccggc aattgccgac gactctggtc
     8701 tggcggtaga tgtgcttggc ggcgcgccgg gcatttactc cgcgcgttat tccggtgaag
     8761 acgcgaccga tctaaagaat ctgcaaaaac tgctggaaac actgaaagac gtaccggatg
     8821 accaacgtca ggcgcgtttc cactgcgtgc tggtatatct gcgtcacgcg gaagatccga
     8881 ctccgctggt atgccacggt agctggccgg gcgtgattac tcgtgaacca gcgggcactg
     8941 gtggctttgg ttatgatcca atcttcttcg taccttccga agggaaaacc gccgccgaac
     9001 tgacccacga agaaaagagc gccatttccc accgtggtca ggcattgaaa ctgctgctgg
     9061 acgctttacg taatggttaa attaccgccg ctgagtctct acattcacat cccgtggtgc
     9121 gtgcagaaat gcccgtactg cgatttcaac tctcacgcgt tgaaaggaga agtgccgcac
     9181 gacgattacg ttcagcatct gctttgcgat ctggacaacg atgtggctta cgctcagggc
     9241 cgtgaagtaa agacaatctt tattggcggt ggtacgccga gcctgctttc cggcccggcg
     9301 atgcaaacgc tgctggacgg cgtgcgtgcg cgtttgccgc tggcagcgga tgcagaaatt
     9361 actatggaag cgaaccctgg tacggtagaa gccgatcgct ttgtcgatta tcagcgtgct
     9421 ggtgtgaacc gcatctctat tggtgtgcag agttttagcg aagacaagct gaaacgactt
     9481 gggcgtattc atggcccgca agaagcgaaa cgcgcggcaa agctggcgag cgggctgggg
     9541 ctgcgtagct ttaacctcga tttgatgcat ggactaccgg atcaatcact ggaagaggcg
     9601 cttggcgatc tacgccaggc cattgaactg aatccgccgc atctttcctg gtatcaactg
     9661 accatcgaac ctaatacgct gtttggttca cgcccgccgg tactgccgga cgacgacgcg
     9721 ctatgggata tattcgaaca ggggcatcag ttattaaccg cagcgggtta tcagcaatat
     9781 gaaacttccg cttacgccaa atccagttat cagtgccagc acaatctcaa ctactggcgc
     9841 tttggcgact acatcggtat tggctgcggc gcgcacggca aagtgacctt cccggatggg
     9901 cgcattctgc gtaccactaa aacgcgtcat ccgcgtggtt ttatgcaagg aaggtatctg
     9961 gaaagccagc gtgatgtcga agccgcagat aagccgtttg agttctttat gaatcgcttc
    10021 cgtctgctgg aggccgcgcc gcgcgtggag tttattgcgt ataccgggct ttgcgaagat
    10081 gtgattcgcc cacagttaga cgaggcgatt gcccagggtt atctcaccga atgtgcggat
    10141 tactggcaga taacggaaca tggtaaatta ttcttaaact cacttcttga attattcctc
//
LOCUS       DAZFJF010000148        10166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFJF010000148 DAZFJF010000000
VERSION     DAZFJF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.149"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..646)
                     /gene="yncG"
                     /locus_tag="ACHKAU_004232"
     CDS             complement(29..646)
                     /gene="yncG"
                     /locus_tag="ACHKAU_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO5533186.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            912..2411
                     /gene="ansP"
                     /locus_tag="ACHKAU_004233"
     CDS             912..2411
                     /gene="ansP"
                     /locus_tag="ACHKAU_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO5533187.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2526..3587)
                     /gene="yncE"
                     /locus_tag="ACHKAU_004234"
     CDS             complement(2526..3587)
                     /gene="yncE"
                     /locus_tag="ACHKAU_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HFO5533188.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKMLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3829..5931
                     /gene="pqqU"
                     /locus_tag="ACHKAU_004235"
     CDS             3829..5931
                     /gene="pqqU"
                     /locus_tag="ACHKAU_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO5533189.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRQNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5967..6632)
                     /gene="mcbR"
                     /locus_tag="ACHKAU_004236"
     CDS             complement(5967..6632)
                     /gene="mcbR"
                     /locus_tag="ACHKAU_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO5533190.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6830..7867)
                     /gene="curA"
                     /locus_tag="ACHKAU_004237"
     CDS             complement(6830..7867)
                     /gene="curA"
                     /locus_tag="ACHKAU_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO5533191.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8048..8566
                     /gene="mddA"
                     /locus_tag="ACHKAU_004238"
     CDS             8048..8566
                     /gene="mddA"
                     /locus_tag="ACHKAU_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO5533192.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8563..9012
                     /gene="ydcZ"
                     /locus_tag="ACHKAU_004239"
     CDS             8563..9012
                     /gene="ydcZ"
                     /locus_tag="ACHKAU_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5533193.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9013..9246)
                     /gene="ydcY"
                     /locus_tag="ACHKAU_004240"
     CDS             complement(9013..9246)
                     /gene="ydcY"
                     /locus_tag="ACHKAU_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO5533194.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9332..9505)
                     /gene="ortT"
                     /locus_tag="ACHKAU_004241"
     CDS             complement(9332..9505)
                     /gene="ortT"
                     /locus_tag="ACHKAU_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO5533195.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9700..9795
                     /gene="yncL"
                     /locus_tag="ACHKAU_004242"
     CDS             9700..9795
                     /gene="yncL"
                     /locus_tag="ACHKAU_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO5533196.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2459 a         2481 c         2472 g         2754 t
ORIGIN      
        1 taaataacat aatcttctgg tatcccactc aaataatttc attccttttt aatacttctt
       61 gtaatttcgg taactgacat acagcatcag cgatcgcgct gatattctgc gcattatcct
      121 gaaaccattc atgccccggc ccccatgtgc gcatggtgca gagataacag tccaccagcg
      181 ttagttgctc accaaaggca taaggttcag cggtcaattg tgaattcagc cagatataaa
      241 gtgatttgcg atattcaatt acattcttct ttaactgttc aggtgcgtca gatgcccaac
      301 gctcagggta atcggcgaaa gtgaatgttg gatagacatt agcaaccagc cagaccaata
      361 gccgttgaaa ctgctgacgt tcggcgcgcc caaccggtgg cgcaaggtcc gggcgacgat
      421 cgagaaccat aaatgcaatc gccgctgtct ccgtcataat ctcgtcattt tccagcgcca
      481 gtgtcggaac ctgacacaac gggttcaggg ttttcaataa ctcgcgtgaa gctccctcgt
      541 ggtcaaaacc actgacatca acgaactgat aaggaatatc agccagtgtt agcatcagct
      601 cactgattgt agagccccat ccgggtacgc catacacctt aatcatatgc cctcacaatc
      661 ttttaaagat taagtgtagg acagtacaca ggttaaccag caaaaagatt tgtcattttt
      721 tcgtcagttg tattattccc cttcaggatt gtaaggggat gttttgcatc gtttatcgat
      781 aacgggtagg atgctgcgcc gatagtttta tacccataaa gaataatggt gataactatc
      841 atcgccagga tgaataaaca ttgttcatgg caacttatat gactttttca ttaaagcaat
      901 cagggagagc aatgagtaaa cacgacaccg acacttcaga tcaacacgcc gcgaaacgcc
      961 gctggcttaa tgcccacgaa gaggggtatc acaaagcgat gggcaatcgc caggtacaga
     1021 tgatcgccat tggcggcgcg attggcaccg gcttgttttt aggtgcagga gcccgactgc
     1081 aaatggcggg tccagcattg gcactggttt atttaatttg tggcttgttt tcgtttttta
     1141 ttctgcgtgc attgggtgag ctggtgctac accgcccttc cagtggcagt tttgtctctt
     1201 atgcccgtga gtttttgggt gagaaagccg cttatgttgc tggctggatg tacttcatca
     1261 actgggcgat gacggggatt gttgatatta ccgccgtcgc tctgtatatg cattactggg
     1321 gtgcgtttgg cggcgtgccg cagtgggtct ttgcgctcgc tgcacttacc atcgttggca
     1381 ccatgaatat gatcggtgtg aaatggtttg cggagatgga gttctggttt gcgcttatta
     1441 aagtgctcgc cattgtgacc tttttggtcg tgggtacagt gttcctcggt agtggtcagc
     1501 cgctggatgg caacaccact ggctttcatt taattaccga taatggcggc ttcttccccc
     1561 acggtttgct gcctgctctg gtgttgattc agggcgtagt gtttgctttt gcctccattg
     1621 aaatggtggg tacagctgcc ggagaatgta aagatccgca gaccatggtg cctaaagcga
     1681 ttaacagcgt gatttggcgt attggcctgt tttacgtcgg ctccgtggtg ttgctggtta
     1741 tgctattgcc gtggagcgcg tatcaggcgg ggcaaagtcc gttcgtgacg tttttctcta
     1801 aactgggtgt gccatatatc ggcagcatta tgaacattgt ggtgctgacc gctgccctct
     1861 ccagcctgaa ctcaggtctg tactgcaccg gacgtattct gcgctcaatg gcgatgggcg
     1921 gttccgcacc gagttttatg gcgaaaatga gtcgtcagca tgtgccgtat gccgggattc
     1981 tggcgacact agttgtgtat gtcgtcggcg tattcctcaa ctatctggtg ccgtcgcgcg
     2041 tatttgagat tgtgttgaac ttcgcgtcgc tgggaatcat cgcttcatgg gcgtttatca
     2101 tcgtgtgcca gatgcgcctg cgtaaagcaa ttaaacaagg caaagcagcg gatgtcagtt
     2161 ttaaactgcc tggcgcgccc ttcacttcct ggctgacatt actgttttta ctgagtgtcc
     2221 tggtgctgat ggcgttcgat tacccgaacg ggacttatac tatcgcggcg ctgccgatta
     2281 tcggtattct gctggttata ggctggtttg gtgtgcgcaa acgcgttgct gaaattcaca
     2341 gcactgcgcc agtcgtcgag gaagatgaag aaaaacagga aattgtgttt aagcctgaaa
     2401 cggcgagcta attgcaaaat tgcccggcta cgtgccgggc aaatttagat tactgacgaa
     2461 ggtgtgccgc atgcggcgtg ataaaagtca ggcaagtctc tccgttttca ccctcgccac
     2521 atctattaca gcgcaatacg aatcacatcg tccggctggg tagcttcctg ctgtttagtg
     2581 gatttttgtt tcacactgac atacagcatt ttgccatcgg cagacagcgc caggctgttt
     2641 ggatgagtcg gcgtatcgaa cgttttcacc actttatagc ttttcgcgtc aatcacactg
     2701 actttacctg cctgacgatg cgttacgtag gcttcattac gcgctgggtt aaacagcaca
     2761 gccagtgatt ctggtgccgc aaccttcgcc agaatattgc cattacgggt atcgaccact
     2821 aacacttctg cggctttaga atcggtgata aatgcacgct gcctggcggt atcaaggcta
     2881 atgttgataa agaagtgctc tttgccgtca tccagcagct ttttacggct gaggatttta
     2941 ttgtcggcgg tgtcgatggt aatcaattcg ccgtcagcgt tagtggtgta aagacgtttg
     3001 cctttgctat ccagcgccag accggtactc attttaccgg tgttctggat ggcggttttc
     3061 agtttgatat tctcgccatc aacgacccaa atcacgctct ctttaccaat accactgatg
     3121 taaacggtgt tcgtggcatc atcagctacc agctcacgcg gttgcagcgg gcgcacctct
     3181 tccgtgcgct tacgatcatc cagcaccaga cggcctttca cctcgcccgt tttggcatct
     3241 atcgccgtga ccgcgctgtt tacggtgtta ccaaaccaca acgtctgagt cgtgttattg
     3301 atggtggcac caaacggctt gagatcgtta tggatcgcct gcgtcacttc cagagtgacc
     3361 ggatcaagac gataaaccac gccaccttta tccagtttgc ggctttgcga agtggcgagc
     3421 cacagtgcgt tttcttgctg gctataagcc atttcgtagg cacctttacc taccgcttta
     3481 cgcagcattt cttctgcggc ctgcgtactg aatgatgaag caacaagcaa tgaacctaac
     3541 agtaatgaac cacgcaggcg cgatgaaaac agatgacgta aatgcatgac gactcccttt
     3601 gatgaaccga tatttttgct gcttcacaat gcttcgctaa gcgtaatcat cgttacgctc
     3661 ttgttataaa tgggaatggt aatcattatt ttctcaaatg tggagcattt cttgccgtta
     3721 cgactacaat tttctcaatt acttaacgta tcgtgccgtt tcgttttcca taaatttaca
     3781 atttcattac cataacggcg ttttcccatt cttaacctgg cttttttcat gaagattttt
     3841 tccgtccgac agaccgtttt gcccgcactg cttgtccttt cccccgttgt ttttgccgct
     3901 gatgaacaga ctatgattgt cagtgccgca ccgcaggtgg tttcagaact ggatacccca
     3961 gcagcagtaa gcgtggtgga tggcgaggag atgcgcctgg caacaccgcg cattaacttg
     4021 tccgaatcac tgaccggcgt gcctggtttg caggtacaaa accggcagaa ctatgcgcaa
     4081 gatttacagc tgtcgattcg cggatttggc tcccgctcca cttacggtat tcgcggtatt
     4141 cgcctgtatg tggacggtat tcccgccacc atgcccgacg ggcaagggca aacatccaac
     4201 atcgatttaa gcagtgtgca aaatgtggaa gtgctgcgtg gccccttctc tgccctgtat
     4261 ggcaacgcgt ctggtggggt aatgaatgtc accacccaga ccggacaaca gccaccaacc
     4321 attgaagcca gtagttacta cggcagtttt ggcagctggc gctatgggct gaaagcaacg
     4381 ggcgcaacgg gagacggcac acagcctggc gatgtcgatt acaccgtctc aaccacgcgt
     4441 tttacgaccc acggctatcg tgaccatagt ggcgcacaga aaaatttagc taatgccaaa
     4501 ctgggcgtac gcattgatga agccagcaaa ttaagtctga ttttcaatag tgtggatatc
     4561 aaagcagatg acccaggtgg gctaaccaaa gcagaatgga aggctaatcc acaacaagcg
     4621 cctcgtgcag aacagtacga cacgcgaaaa accatcaagc aaactcaggc tgggttgcgc
     4681 tatgagcgta gcctgagcgc gcaagatgat atgagtgtga tgatgtatgc cggagagcga
     4741 gaaacgaccc agtaccagtc aatcccgatg gccccccaac ttaacccgtc acatgcgggc
     4801 ggcgtgatta ccctgcaacg ccattaccag gggatagaca gccgctggac acaccgtggt
     4861 gaactgggcg ttccggtcac gttcactacc ggcctgaact acgaaaacat gagtgaaaac
     4921 cgcaagggct acaataactt ccgccagaat agcggcatgc cggagtacgg gcaaaaaggt
     4981 gagttgcgtc gcgacgaacg caatctgatg tggaacatcg atccctattt acagacgcag
     5041 tggcagctga gcgaaaaact gtcgctggat gctggcgtgc gctacagctc cgtgtggttt
     5101 gattccaacg accattacgt tactccgggt aacggcgatg acagcggtga tgccagttat
     5161 cataaatggc tacctgccgg ttcgttaaaa tatgcaatga ccgatgcctg gaatatctat
     5221 ctggcagccg ggcgaggttt tgaaacgccg acgattaatg agctgtctta tcgtgctgat
     5281 gggcaaagcg gtatgaactt tggtttaaaa ccatccacca acgatacaat tgagatcggc
     5341 agtaaaacgc gtattggtga tgggctgctt agtctcgcat tgtttcagac cgacactgat
     5401 gatgaaattg ttgtcgatag cagtagcggt gggcgtacga cttacaaaaa tgccggaaag
     5461 acccgtcgtc aaggcgctga actggcatgg gatcaacgtt tcgcaggaga ttttcgcgta
     5521 aacgcgtcct ggacctggct tgatgcgacc tatcgcagca atgtttgcaa tgaacaggat
     5581 tgtaacggta atcggatgcc agggatcgcc cgtaatatgg gctttgcgtc gataggttat
     5641 gtaccggaag atggttggta tgcaggcacg gaagcgcgtt atatgggcga tattatggca
     5701 gatgatgaaa atacggcaaa agcgccgtct tatactctcg tcggcttatt caccgggtat
     5761 aaatacaatt accacaattt aactgtggat ttatttggtc gtgtcgataa tttattcgat
     5821 aaagaatacg ttggttctgt cattgtcaat gagtcaaacg ggcgatatta cgaaccttcg
     5881 cccggacgaa attatggtgt cggcatgaat attgcgtgga gatttgagta attaccttga
     5941 tgcccggtat ttgccgggca tttactttaa cgattgtatt gctggtataa aatagcaata
     6001 ttttgttgca taatttcagc aagacaatgt ctgcatccct ctttgtcttt tgcttttaac
     6061 gcagcgagta acagatgata gttttctata tgctcccgta attccgctgg attaatcgct
     6121 tcatagagat aatgtaaacc aggccccatc ctgacccaca gttgctcaat catctcacac
     6181 aggatgggca tatttgagcg atgataaatc gctaagcgaa atagcctgtt tacattaatg
     6241 atttgttcca tgtcgccctt ttcttgcgcc tgctgtaatt tctcgagcaa ttcctggagt
     6301 tccgccaggt cttgcggggt gaggttttca acagccagag caactgccat taattccagt
     6361 tcgtagcgga tccgattgat ttcgtctaat tgacgtttcc tcacttccgg aactgtaaat
     6421 gcttgtgcag gtgcgacaga aagcgcattc accgaaacca gacgtaataa tgcttcacgc
     6481 acaggtgtaa tactcatacc taattgctcc gccagatttt tagtaatcag gcgtgcgcca
     6541 ggttttaatg cgccaatact aagctgatgt ttcaggtcgt tctcaacctg cagagtcaaa
     6601 ctgacatgtt tcatttttcc cattccaggc attttaattc cttcgcgtgt tttctgtcca
     6661 accctgcctg cacggcagaa ggagttcacc tggtgtatta aagtgaataa tcataaacca
     6721 cccacctttg ttgttattag cagcagaaca atgacgtggt tagttaatta aagctgaaaa
     6781 taagcttttt gcctggattt ttatggcggc ttacgccgcc gttgaaactt taatcatcac
     6841 ccgcgacgcg gatcaccact ttgccgaagt ttttaccctt cagcaggccg ataaacgtct
     6901 gtggcgcatt ctctaaacca tcagtaatat cttcgtggta gtggatttta tcctctttca
     6961 cccattgccc catctccttc tgaaactcat ggatgcggtg accataatcc tgagcgataa
     7021 taaacccttg caagcgaata cgttttttca gcaccgttgc catcaacagg ggtaaacgat
     7081 ccggaccggg tggtagctct gtagcgttat agctgctcac taagccacaa acgggaatgc
     7141 gcgcagatgt attaagtaat ggtagcactg catcaaatac cttaccgccc acgttttcat
     7201 aatagatatc aatacctttt gggcacgctt tcaccagttg ttcggcaaaa tcatccgcgt
     7261 ggtgatcaag acaaacatcg aagcccagca cctcgatagc atggcggcat ttttccgcgc
     7321 caccggcaac ccccaccacc ctgcaacctt taagtttgcc gatttgcccc accgttgctc
     7381 ccacaggacc tgtcgccgca gctaccacca acgtttcgcc ctctttaggc tgaccgatat
     7441 ccagcagccc catataagcg gtaaagcctg gcatccccag cacacccagc gaccacgatg
     7501 gattttgcgg atgatcgcca agtttcacca gatcatcacc actggatatg tcatagtcct
     7561 gccatccact gtagcccagc acccagtcgc caggctgata atcaggatga ttcgactcca
     7621 caacacggct caccgtgccg ccgaccatca cgccgccaat atcaacaggc ggtgaataag
     7681 atggctcatc gctcatacgt ccacgcatat acgggtccag ggacaaataa actgtgcgca
     7741 gtaacacctg tccttcaccc ggtgtggcga catcatcttc ttcaagacgg aaattctccg
     7801 gaaccggtgc gccatgtgga cgcgaggcca gaacccaacg tcgattacgc tgcttttgtt
     7861 gcctcataat gctctcctgt gcttttggca tgaatccatc aatagtagca ggctcacggg
     7921 attggttttg tttgcagccg tcacatcccc tctgcaacac gcaataattt catcgaggaa
     7981 tccgttgctt cgggctgttt tcgatagtat tgataacatc atcatcatta ttttatcgag
     8041 gccagacatg tccatccgtt ttgcccgcaa agccgattgt gctgccattg cggaaattta
     8101 taaccacgcc gtgttgtata cggcggctat ctggaatgac caaacggttg atgcagataa
     8161 ccgcattgcc tggtttgaag cgcggaccat agcaggttat ccggtgctgg tgagtgaaga
     8221 agatggcgta gtgacgggat atgcctcgtt tggcgactgg cgtagtttcg atggttttcg
     8281 ccataccgtg gaacattcgg tttatgtcca tcccgatcat cagggcaaag gtctggggcg
     8341 taaattgtta agccgattga ttgatgaagc gcgggattgc gggaagcatg tcatggtcgc
     8401 cgggatcgaa tcgcaaaatc aggcctcgct gcatctgcac cagtcgctgg gatttgtcgt
     8461 caccgcacaa atgccgcagg tgggcactaa atttgggcgc tggctggatc tgacatttat
     8521 gcagttgcaa ctcgacgaac gcactgaacc ggacgcgatt ggatgaatca gtcgctcacc
     8581 cttgcttttt tgattgccgc cggaattggt ctggtagtcc agaacacgct gatggtacgc
     8641 atcacccaga cctcctctac cattctcatc gccatgttgc tgaactcact ggtggggatt
     8701 gtgctctttg tttctatttt atggtttaaa caaggcatgg cggggtttgg cgaactggtg
     8761 tccagcgtgc gctggtggac acttataccc ggcttgctgg gatcgttttt tgtcttcgcc
     8821 agtatcagcg gatatcagaa tgtgggagcg gcgacaacta ttgcagtact ggtcgccagc
     8881 cagctaattg gcggtctgat gctggatata ttccgcagtc atggcgtacc actacgggcg
     8941 ttgttcggcc caatttgtgg cgcaatttta cttgtggttg gcgcctggtt ggtagccaga
     9001 cgctcatttt aatcagagga tggtgccgcc tttggttaac tgttcgtggc gcgcttccat
     9061 atcttcttta tgcttgcgac cgtgatgagc aatagcggtg cgcaaacgct gctgctgggt
     9121 gtagcgatct tcccgactta actccgcgtc atcgctcagg gcgattaata attcgttcat
     9181 atgggcaatg acgctctctt caatcgcggc atccacgcgc gcgatgactt catccagatg
     9241 cgacatactt tctccttaac cacttgtgaa atttatcgat tcatccaaat gatataagtc
     9301 tgcgtggtgc cgtcaagcca gtgagtaaga tttaaaacag tgaaaacaac agcgccaccg
     9361 ggaaactcat cggccatgtt gcccccacca gaaaggcgct taagaaacgg atgcgtttct
     9421 tatcgtgaga aagaaaccag gtgataagaa atgcgattgc tgccataacc gcataaaaaa
     9481 caagcatatg ttgatagaga gacatgtacg ggcgtccagc cagatgaata aaacgcgcgc
     9541 aaatatgcgt cattttttga cgtgcatcaa gttttttcac cagtttgaga aaaaatcatc
     9601 aaacagcaac ttgcccttat ccatcttcag aatgagtatt attgcggatt ttcttaaccc
     9661 gtactataca cataggggca atgataaaag gtggcaaaaa tgaatgtttc cagtagaact
     9721 gtagtactga taaatgtctt tgctgctgtt ggtttgttta ctcttatctc tatgagattt
     9781 ggctggttta tttgatgtag tgattttgca agcagcacgt actgtcagag aaatcaactc
     9841 tctgacagta cgcaagatct tctcatactc tccaggttta caacattatc cttaatattt
     9901 acttcttttt gctggcatct acattgaaat gagtagaaaa catcccccta ctgtcgtcta
     9961 tacaaacagc ttactgattc aaacggaatc ttgttttcac atttttctat catgaatact
    10021 ttatacgcgc gggagtctat aaatgcttta tactttcaag gaataacgta aatataaaaa
    10081 gatggcgtaa agtatatgca tggcaggatg tttgcaacct ccttgatgac gctgccccat
    10141 tgaaattgca aaccccgccc tggtta
//
LOCUS       DAZFJF010000149        10130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFJF010000149 DAZFJF010000000
VERSION     DAZFJF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.150"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            213..1586
                     /gene="aroP"
                     /locus_tag="ACHKAU_004243"
     CDS             213..1586
                     /gene="aroP"
                     /locus_tag="ACHKAU_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO5533197.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1629..2483)
                     /gene="ampE"
                     /locus_tag="ACHKAU_004244"
     CDS             complement(1629..2483)
                     /gene="ampE"
                     /locus_tag="ACHKAU_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO5533198.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2480..3031)
                     /gene="ampD"
                     /locus_tag="ACHKAU_004245"
     CDS             complement(2480..3031)
                     /gene="ampD"
                     /locus_tag="ACHKAU_004245"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO5533199.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3119..4012
                     /gene="nadC"
                     /locus_tag="ACHKAU_004246"
     CDS             3119..4012
                     /gene="nadC"
                     /locus_tag="ACHKAU_004246"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO5533200.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4215..4655
                     /gene="ppdD"
                     /locus_tag="ACHKAU_004247"
     CDS             4215..4655
                     /gene="ppdD"
                     /locus_tag="ACHKAU_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO5533201.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4665..6050
                     /gene="gspE"
                     /locus_tag="ACHKAU_004248"
     CDS             4665..6050
                     /gene="gspE"
                     /locus_tag="ACHKAU_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO5533202.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMQHGE"
     gene            6040..7242
                     /gene="hofC"
                     /locus_tag="ACHKAU_004249"
     CDS             6040..7242
                     /gene="hofC"
                     /locus_tag="ACHKAU_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO5533203.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7277..8320)
                     /gene="guaC"
                     /locus_tag="ACHKAU_004250"
     CDS             complement(7277..8320)
                     /gene="guaC"
                     /locus_tag="ACHKAU_004250"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO5533204.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8476..8520)
                     /locus_tag="ACHKAU_004251"
     CDS             complement(8476..8520)
                     /locus_tag="ACHKAU_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533205.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8545..9165
                     /gene="coaE"
                     /locus_tag="ACHKAU_004252"
     CDS             8545..9165
                     /gene="coaE"
                     /locus_tag="ACHKAU_004252"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO5533206.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9165..9908
                     /gene="zapD"
                     /locus_tag="ACHKAU_004253"
     CDS             9165..9908
                     /gene="zapD"
                     /locus_tag="ACHKAU_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO5533207.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9918..10115
                     /gene="yacG"
                     /locus_tag="ACHKAU_004254"
     CDS             9918..10115
                     /gene="yacG"
                     /locus_tag="ACHKAU_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO5533208.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         2489 a         2665 c         2594 g         2382 t
ORIGIN      
        1 tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc gggtgcattc
       61 gctgcctcat accattattc ttgatctgac ggaagtcttt ttgtaacaat tcaaacttct
      121 ttgatgtaaa caaattaata caacaaacgg aattgcaaac ttacacacgc atcactgcgt
      181 agatcaaaaa aacaaccacc gcacgaggtt tcatgatgga aggtcaacag cacggcgagc
      241 agctaaagcg cggccttaaa aaccgccata ttcagcttat cgcgctgggt ggcgcgatag
      301 ggaccgggtt attcctgggt agcgcctccg taatacagtc cgcagggcca gggattatcc
      361 tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg
      421 tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac tggggcagtt
      481 ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt gccatggctg
      541 agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatcccc acctgggttt
      601 ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg
      661 gcgagatgga gttctggttt gccattatca aagttatcgc ggtggtagcg atgatcatct
      721 tcggcggctg gctgctattc agtggcaacg gcggcccgca ggcgaccgtt agcaacctgt
      781 gggatcaggg tggtttcctg ccgcacggct tcaccgggct ggtgatgatg atggcgatga
      841 tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa gctgataacc
      901 cggagcaaag tataccgaaa gcaactaacc aggttatcta ccgcatcctg attttctata
      961 ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc gccgatacca
     1021 gtccgtttgt gctgatcttc catgagttag gcgatacctt tgtggcgaat gcgctgaaca
     1081 tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc aacagccgta
     1141 tgctgtttgg tctggcacaa cagggtaatg cgccaaaagc gctggcgtct gtcgataaac
     1201 gtggtgtacc agtaaatacc attctggtgt ctgcactggt aacggcgttg tgcgtactga
     1261 ttaactacct tgccccagag tccgctttcg gactgttaat ggcgctggtg gtatctgcac
     1321 tggtaatcaa ctgggcgatg attagcctgg cgcatatgaa attccgtcgc gccaagcagg
     1381 aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac tggatctgcc
     1441 tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg gcgatttcgg
     1501 tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa
     1561 ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca gggtgagggc
     1621 aataatcttt acaccaacgc cccgtaaatc gtcagtagtg caatcaccac cacgaccacg
     1681 aacgaggttt tcttcgccat tgaaaccgct gccctcggcg tctccacctt atcgacatgc
     1741 ggttcacgcg ccagagagaa ctgcgccaga cgcgttaaca cctgatactg cgaagtatgg
     1801 aaatcaccca gcgaagcaaa ccaggccggt aacgctttct caccatgacc gatcaaggca
     1861 tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac ggcatcaatg
     1921 ccggactgta aacgatgatg cggcgtctga tatcgcgcca gccagtattg ccatgcacgc
     1981 aaaaacgcat accccatcag cgtaacgggt ccccaggttc cccccacaat cagccagaac
     2041 agcggtgcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag ctcacgcaaa
     2101 tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc gccagccatc
     2161 gtggcacggg catggctatc attacgtgaa gcagctgtca gataagcatg ataatgaaga
     2221 cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag tagcgtggga
     2281 acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacgcc catcgcaata
     2341 atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa gaacgcttca
     2401 agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc
     2461 agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcacacga aaccgtgccc
     2521 aatcaaatgc aggaccggga tcggttttcc gatccggcgc aatatcacaa tggcccgtca
     2581 tgtttttagc gatatccgga tagcaatcaa tcagtgcccg cgtaaccgcc gcaagctgtt
     2641 gatactgcgc atcggtatac gccagcgtat cggtgccttc aagctcaatc ccaatagaaa
     2701 aatcattgca gcgttcgcgc ccctgatact gagagactcc cgcatgccat gcacgtttat
     2761 cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga gcggagacgc
     2821 gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata
     2881 atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg tgcaccacca
     2941 gcagggtggg tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga acgcggcgcg
     3001 cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt ggtgctaata
     3061 cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga gttttaacat
     3121 gccgcctcgc cgctataacc ctgacacccg acgtgacgag ctgctggaac gcattaatct
     3181 cgatatcccc ggcgcggtag cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc
     3241 caacaatgat attacggcaa aacttttacc ggaaaattct cgctctcatg ccacggtgat
     3301 cacccgcgag aatggcgtct tttgcggcaa acgctgggtt gaagaggtgt ttattcaact
     3361 ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca tcaatgccaa
     3421 tcaatccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc
     3481 gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact atgtcgaatt
     3541 gctggaaggc accaacacgc agttgttgga tacgcgcaaa accttacccg gcctgcgttc
     3601 agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg ggctttctga
     3661 tgccttcctg atcaaagaaa accatattat tgcctccggc tcagtgcgcc aggcggtcga
     3721 aaaagcgtcc tggctgcacc cggatgcgcc agtagaagtc gaagtagaga atctggaaga
     3781 acttgatgaa gccctgaaag caggagccga tatcatcatg ctggataact tcgaaacaga
     3841 acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa
     3901 cgtcactgac aaaacactgc gtgaatttgc cgaaacgggc gtggacttta tctccgtcgg
     3961 tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct aatagttgac
     4021 agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc ttcgtaacgc
     4081 ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg
     4141 tttcgttttt tgcccctcgc ctgtgctgat ggcgtttgcc aaagtagcgc caactaaatc
     4201 aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta
     4261 ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac tacctgcgca
     4321 aagccgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt
     4381 gcgcgctgga acatggtgga ttagatacct gcgacggtgg cagcaatggc attccctcgc
     4441 ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg
     4501 ggcaagaaag tctcaatggg ctaagcgtcg tcatgacacc gggttgggat aacgcaaacg
     4561 gcgtcaccgg ctggacgcgc aactgcaata ttcaaagtga cagcgcattg catcaagcct
     4621 gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat attccacagc
     4681 tcactgccct gtgtctgcgt tatcatggag tcttgctgga tgccagcgaa gaggtggttc
     4741 atgttgcggt agtcgatgca ccttcgcatg agctactgga cgcattgcat ttcgctacca
     4801 ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggtcac gccagtcgca
     4861 cacaacagac attgcccgta gctgttcagg agaagcatca gcccaaagca gagttgctaa
     4921 ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga tattcatatc gaaccagcgg
     4981 acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggatgttt
     5041 caccggatgc cggagtcgca ttaaccgcca gattaaaagt gctgggaaac ctggatattg
     5101 cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga aacgccgtct
     5161 catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac
     5221 agcaggtgag ccaggcactg gatgtcaaca cgcttggaat gcagccgtta caactggcgg
     5281 actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc cctacaggca
     5341 gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct gaataccgct gacattaata
     5401 tttgtagcgt cgaagatccg gttgagatcc ccatagccgg actaaaccag acgcaaatcc
     5461 atccgcgtgc cggactcacc tttcagggcg tgttgcgtgc gttattgcgc caggatcctg
     5521 acgtcatcat gatcggagag atccgcgatg gcgaaacagc agagatcgct attaaagcgg
     5581 cgcaaactgg tcacctggtg ttgtctaccc tacacactaa ttccacctgc gaaacgctgg
     5641 tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt acgctggtaa
     5701 tagcccagcg tctggtacgc aaactttgcc cacattgtcg ccggcagcaa ggggagccca
     5761 tccacattcc agacaatgta tggccatcgc cgctgcccca ctggcaggca cccggttgtg
     5821 tacattgcta ccacggtttt tatggtcgta cggccttatt tgaagttctg cccataacgc
     5881 cggtcattcg tcagcttatt tccgctaata ccgacgttga atcgctggaa acgcacgcac
     5941 gacaggcggg tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa
     6001 ccacctttga agagttaatc cgcgtactgg ggatgcagca tggcgagtaa gcaactctgg
     6061 cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc
     6121 cgtgctttgc tgctcatggc actacagcaa cagatggtta ccccacttag cctgaagcga
     6181 atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg
     6241 gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggcactgct ggcggaacag
     6301 catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc
     6361 attgcttttt ccaatgcctt attaccctgg tcagaggtat ttccgccact ctatcaggcg
     6421 atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt
     6481 cagcaaaaag cccagcgtca gttgaccgac aaagtgaaat cagcgttacg ttatcccatc
     6541 atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag
     6601 tttgccgcta tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg
     6661 acgctggcag actttagtgg cgaatggagc tggctgctgg tgttgttcgg ctttctgctg
     6721 gcgatagcca ataagttgct gatgcgccga ccgacctggc ttatagtgcg gcagaaattg
     6781 ctgttacgca tcccgattat gggttcactg atgcggggac aaaaactcac gcagattttt
     6841 acgattctgg cgctgacaca aagtgcaggc attacttttt tgcagggcgt agagagcgtc
     6901 agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc
     6961 agtaacggtc atcccatctg gctggcgcta aaaaataccg gggagtttag cccgctctgt
     7021 ttgcaattag tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc
     7081 gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa
     7141 ccggcgttgc tgatcataac gggaggaatt atcggtacgc tggtggtggc gatgtatctg
     7201 ccaattttcc atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca
     7261 caccagcgtt ggaagattac aggttgttga agatgcggtt ttcttgttcc tgcacacgga
     7321 taaatgtggt gcgcttggtc agttctttca ggcgtgaagc cccaacgtat gtacaagctg
     7381 aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct
     7441 taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg
     7501 cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc
     7561 gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc
     7621 cgccaaaggc ttttgcgaca tcgcccggcg tggtgcagcc accgtcgctg acgatcattc
     7681 cgcccagacc gtgcgccgca tcggcacatt cgattaccgc agaaagctgc ggataaccga
     7741 cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat
     7801 cagcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa
     7861 tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat
     7921 aaccattcgc cacatcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt
     7981 tagttttttc gaaatccgca tcagacgtac cggtagaaac catcacatgt ttcagcacat
     8041 cagcggaaga attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag
     8101 cagtcaaaat atcaaaagaa gccagcgcag aggccataga aaatgtacct acggtgtcca
     8161 tatttgcggc gataattggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt
     8221 gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa
     8281 cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa
     8341 tggctataag ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc
     8401 gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag
     8461 tgagcgaacg tttcatcaat ttcgctttcg tgcactcatt tttaacgttt tgataatcat
     8521 gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc
     8581 agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc
     8641 gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat
     8701 cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag
     8761 cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt
     8821 cagcaagaga cgcaacacca gatccagcaa gctacttccc cctatgtact gtgggttgtg
     8881 ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc
     8941 agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtc
     9001 gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc
     9061 attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac
     9121 tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc
     9181 tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc
     9241 agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta
     9301 atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag
     9361 aacttgaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga
     9421 gccgtattga agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc
     9481 cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga
     9541 gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac
     9601 cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc
     9661 aggcacttac catggtgctg gatttaattc gccagtcggc tcccttccgt aaacaaacca
     9721 gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt
     9781 cgctcgattc acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt
     9841 ttatgccgct ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct
     9901 gttgctaagg agtgaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa
     9961 cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga
    10021 tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg
    10081 aaagcgatga ctggagcgaa gagccaaagc agtgacattt gtctgatgcc
//
LOCUS       DAZFJF010000150        10049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFJF010000150 DAZFJF010000000
VERSION     DAZFJF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.151"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            204..1374
                     /locus_tag="ACHKAU_004255"
                     /pseudo
     CDS             204..1374
                     /locus_tag="ACHKAU_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1457..1714
                     /locus_tag="ACHKAU_004256"
     CDS             1457..1714
                     /locus_tag="ACHKAU_004256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533209.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(2272..3039)
                     /gene="fecE"
                     /locus_tag="ACHKAU_004257"
     CDS             complement(2272..3039)
                     /gene="fecE"
                     /locus_tag="ACHKAU_004257"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO5533210.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(3040..3996)
                     /gene="fecD"
                     /locus_tag="ACHKAU_004258"
     CDS             complement(3040..3996)
                     /gene="fecD"
                     /locus_tag="ACHKAU_004258"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO5533211.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3993..4991)
                     /gene="fecC"
                     /locus_tag="ACHKAU_004259"
     CDS             complement(3993..4991)
                     /gene="fecC"
                     /locus_tag="ACHKAU_004259"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO5533212.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4988..5890)
                     /gene="fecB"
                     /locus_tag="ACHKAU_004260"
     CDS             complement(4988..5890)
                     /gene="fecB"
                     /locus_tag="ACHKAU_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO5533213.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(5935..8259)
                     /gene="fecA"
                     /locus_tag="ACHKAU_004261"
     CDS             complement(5935..8259)
                     /gene="fecA"
                     /locus_tag="ACHKAU_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO5533214.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(8346..9299)
                     /gene="fecR"
                     /locus_tag="ACHKAU_004262"
     CDS             complement(8346..9299)
                     /gene="fecR"
                     /locus_tag="ACHKAU_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO5533215.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(9296..9817)
                     /gene="fecI"
                     /locus_tag="ACHKAU_004263"
     CDS             complement(9296..9817)
                     /gene="fecI"
                     /locus_tag="ACHKAU_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO5533216.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9954..10022
                     /gene="ykiE"
                     /locus_tag="ACHKAU_004264"
     CDS             9954..10022
                     /gene="ykiE"
                     /locus_tag="ACHKAU_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO5533217.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
BASE COUNT         2278 a         2744 c         2850 g         2177 t
ORIGIN      
        1 gtgcgttccc tgtattagca atgttaatat caaagtaaaa agcggcccaa gggtttggct
       61 caagtgttac aactatatgg ggttgagctg atgcgtcaac cattcttttt gtctcgtctg
      121 ctctgaagtg gtgaagtggt ttactgaatt tggccacctg aacagaggtg atatgctcac
      181 ctcagaacaa cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg
      241 aatccgctca actggttgtt gaccagacat acacggtggc agatgccgcc aaagctatgg
      301 atgttggcct ttccacaatg acaagatggg tcaaacaact gcgtgatgag cgtcagggca
      361 aaacaccaaa agcctctccg ataacaccag aacaaatcga aatacgtaag ctgaggaaaa
      421 agctacaacg cattgaaatg gagaatgaaa tattaaaaaa ggctacaacc gcgctcttga
      481 tgtcagactc cctgaacagt tctcgataat caggaaactc agagcgcatt atcctgtggt
      541 cacactctgc catgtgttcg gggttcatcg cagcagctac agatactgga aaaaccgtcc
      601 tgaaaaacca gacggcagac gggctgtatt acgcagccag gtacttgaac tgcatggcat
      661 cagccacggc tctgccggag caagaagcat cgccacaatg gcaacccgga gaggctacca
      721 gatggggcac tggcttgctg gcagactcat gaaagagctg gggctggtca gttgccagca
      781 gccgactcac cggtataagc gtggcggtca tgagcacgtt gctatcccga atcatcttga
      841 gcgacagttc gccgtaacgg aaccaaatca ggtgtggtgc ggtgatgtga cctatatctg
      901 gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa gaaaaccagt
      961 gggctgggcc atgtcgttct cgccggacag caggcttacc atgaaagcac tggaaatggc
     1021 atgggaaacc cgtggtaagc ccgtcggggt gatgttccag gcgatcaagg cagtcattat
     1081 acgagcaggc agttccggca gttactgtgg cgataccgga tcaggcagag tatgagtcgg
     1141 cgtggaaact gctgggataa cagcccaatg gagcgcttct tcaggagtct gaagaacgaa
     1201 tgggtgccag cgacgggcta tgtaagcttc agcgatgcag ctcacgcaat aacggactat
     1261 atcgttggat attacagcgc actaagaccg cacgaatata atggtgggtt accaccaaac
     1321 gaatcagaaa accgatactg gaaaaactct aacgcggagg ccagttttag ttgaccacaa
     1381 cagactacct gaagggagcc gcggtcgcct agcagttgca gtagcaggag atcatccagc
     1441 cgcagtacag gtcacgatga ctctggttaa tgataccggc tttgaccccg tattttccgg
     1501 ctctatcgct gaatcatggc gtcagcagcc gtgcacacca tcctattgtt gtgactggga
     1561 ggctgccacc atgcttcgcg ctttccctct ggcgaaaaag ggagaaggac gggcccgtct
     1621 gccttcactt tatgccagct tcggtaagct gggtgagaca ccgactcata aagatatcat
     1681 tgataacaat cgatccatca actggcctgt ataacgtggc tgccggtgat taagaaagct
     1741 gcacctacct aagtagtagc aaacgcacac tttttagaaa aatcgatggt cagaaactgg
     1801 attagcaatt ccgttccagg gttgcttttg atttacgttg gcgtctgatc attgatttat
     1861 cctcaaaagc ccaacctcat tggtaatgaa ccagctccgt gaatgtccgc tctggcacag
     1921 agcgaaattt tttgatctcc ccccctgaaa tctaaactta gtcatgtcac gtttttgggt
     1981 ttctaaaatt ttaacttcgc gtttttcgtt gccgtaaggg ttatacagaa atgtccgtta
     2041 agcagagttc aaaattgatt gccgtgatca cgactggttt gaaagccgcg cccaagcctg
     2101 tacagctctg gtttgcgttg attatgaacc tgtcagccta aagcaagcgg atggacgatg
     2161 agtattggta atctttcaga gtccggaaaa gttcagcccc agtctgaaca ggcttgctgg
     2221 cgccagtcca gtttcattca gtcgtggttt ggttcttacg gcctgtgcaa tctacctcat
     2281 taggcacatc ggcctgccag ataccggctc ggggtgtatt tccgcttcca cgctgaatac
     2341 tgttctcagc aatcctgggg tcatcacctc ttctggtgtg ccttgcgcca taacatgtcc
     2401 gtttgccatt accaccagtt gatcgcagta ccggctagcc tgattaaggt cgtgcagcac
     2461 agcgaccacc gttttcccct gagtccggag ttcgcccatc aaccgcatca ggtccacctg
     2521 gtgattgata tcaagatagg tggttggctc atcaagtaat acaacgggcg tattctgggc
     2581 caggaccatc gccagaaatg cgcgctggcg ctgaccgccg gaaagctcgg ttaaccgacg
     2641 aacggcaaga tgattgatcc gggtctggtt catggcgaca ttaactcgtg cattgtcttc
     2701 agcggagaga cgcccccaga gtgacagcca gggattacga ccatacgaaa ccagctcctg
     2761 gactgtgatc ccctctggcg ttaaatggtg ctgaggcagc agcgaaagcc tgcgggccaa
     2821 ctggcgcgat gagagcatat ttatgggatt atcgccgaga aatacggtgc cagactgcgg
     2881 cattaaaagc cgcgaaaaac agtttaacag cgtcgatttc ccgcaaccgt taggaccgat
     2941 cagggcggtg atcttccccg ttggcagtga gagtgaaacg tcgttaagta ccttgtctgt
     3001 cccgtaactg accgtcagat tttcagttcg taaagtcatt tatcgcattc tcacaagcaa
     3061 ccagacaaac cacggcgcac cgataatggc ggtcagcacg ccaaccggga gctccagtgg
     3121 gggatgaata attctcgcca gcagatcggc aaccaccaac agcaacgcac ctgtcagggc
     3181 tgaaacaggc agcagtctgc ggtgacgtcc accggtgatg ctacgcatca tatgcggcac
     3241 cacgagacca ataaagctaa tcgggccgca ggcggccacg ccggtagatg tcatggcgac
     3301 agctagtaac aaagcccaga atcgggtatg gggcaccgac acaccgagcg tggtggcgcg
     3361 cgcatcgccg agtgcaagga ggtcgagatc gcggcaaaaa ctcaggctca gcggcagaaa
     3421 taaaatcatc agcgggatgg caatcttcac aaagctccag tcacggcccc ataagctgcc
     3481 ggtcagccac agcagggcgt tgttcacatc ctgtgggcgc gagagcatca gataatccgt
     3541 caggctggcc cagcatgcag aaagcgccac gccggtgagc gccagcttca tcggctggtg
     3601 ggtctttgcc agcatcttca gtaatatcaa ccccgccatg ccgcccgcaa aggccagcag
     3661 cggcagcacc atcacgggca gtgacggcat aagaagtaga gcccccacag aggccaggct
     3721 ggcggcatgg ttaacgccga gaatatccgg tgatgccaga gggttgcgca caatcccctg
     3781 tatcagcacg cccgccacgg cgagggctgc accgacaaac agtgccagca gcaagcgcgg
     3841 cagtcggtac tccatcaata cataataatg ctcgcgtccg gcctgccagt cggtcagcag
     3901 cgcgcgccac ggcacgggga tcactcccat atggagtgat aacagcgcac agcccgccag
     3961 ggcaagggtg atgaaaataa ccagcgcaat tttcatcctc gcctcctcac aagccagaca
     4021 aagcaagggc tgccaatcag cgccagcact gcgcctgcgg gcagatctcc ggggaaggcc
     4081 agcgcgcgtg cgagtacatc tgccagcagc atcagcgtgg cccccagcag catgctcact
     4141 ggcagtacgt tgcgctgatc gaagcctgcc cagaagcgcg ccagatgtgg caccagcaga
     4201 ccgataaacg ccaccggacc tgccacgctg acgcacgcac caaccagaag cagcactaac
     4261 atattgatga ccaaacgtag cctcgtcagg ttcactccca gcgtatgggc ggtgctgtcg
     4321 ctgaggttga gcaggttcag ttgattcgcc agcagcaaca cgacagggac tgcagtgacc
     4381 accaccggca agagctgcca gacatcctgc cagcgggcgt gggacactcc gcctgccagc
     4441 cagtaaaaga tgccgtaagc atgatcttcg gccagcagca gggtgatgcg ggtaaggccc
     4501 atacaaaagg ccgacagcgc gatactcgcg aggatcagtt tgtttctgtc atgggtatga
     4561 cgaaatccgc ctcctgcggt catgaccagc agccagctca cgccgccccc gcatgccgcg
     4621 atgaacgaca gagaatagcc tgcaatcggc gtcggactca gcgcgctggt aagcgccata
     4681 gccagcgccg cgccgctgtt aatgccgagc agtgaaggag aggccattgg gttgtgggtc
     4741 agggtttgca gcagcgtgcc cgcgagcgcc aggcttgcgc cgatcagaac ggcgaccagg
     4801 cttcgtggca aacgaaggtt ttgcaccagc gcttctggta gcgttggcgt gtgtccaggc
     4861 agcagggcgc gggttgcatc tgctccggaa acaggaatgg ccgagtagca aaacagactc
     4921 agccagaaaa taataataag tgctgcaacg ggaagccccc acagcagcac cgggtgtttt
     4981 atcgcggtca tttcacaacg gtaagcggct gatggtggaa gatttttacc gtgtcagcgg
     5041 caatacgctc tgcagcaaaa ataccgcgca tccgcgccca ggtgttactg tcgaccgaag
     5101 caacctgctg cttctgcgcg gcggttaaca tctgccagag cggatcttgt tgccagcgtt
     5161 taacaatgct ctcttcgcga tagtgggcaa ccagcagcca ggcaggattg accgccagca
     5221 gttgctccag gccgatggac ggcatggacg cacccgccat cgcagcggga acgttcagcc
     5281 ccagagaggc cagcacgctg ccggtccagg tctcctgagt atgcaggttg aattgctgtt
     5341 cgcgtgatgt gccaaaggcc acgcgtgtcc ctttgggaag ctggctggcc cactgcgcca
     5401 tcctctcttt atgttgttcc aggcgtgcct gcatctctcg ctttttaccc accatttcgc
     5461 cgatgatagc cgcagattgc aaattttcag cgtaggtttc gttgcgggac ttaagcagca
     5521 gtaccggcgc gatttgctgc aaggcgatgt aaacccccgc atggcgactg ctgtcggcaa
     5581 tgatcaggtc tggtttcaga gcggcaatgg cttccaggct cggctgcgcg cgcgttccga
     5641 cggactgcca cggtttcagg tgcgcacgca cttcgggcag gatgcgtttt gcatcgttat
     5701 cgtcggcaat accgatcggg ctgacgtcca cggcggccag cgcatcggcg aacgagagtt
     5761 ccagcaccac aatccgttgt ggcgtttttt cgagtgtaaa cgtgccgtgt tcgtcctgaa
     5821 ccgtggcggc aaaggcgtgg ctgatcacca gcagcaggcc tgcaaaaaca aaacggataa
     5881 atgccaacat aatcacattc cagctaaaag cccggcaagc cgggcgttaa cacatcagaa
     5941 cttcaacgac ccctgcatat acagcgtgcg cggctggcct gcatagatgc ctttgttgtt
     6001 gtcgtcataa gagcggatga agtagtcctg gtcgaagatg tttttcacac cgaatgccag
     6061 gttcagatct gccatctgcg ggccaaaatc atacgccacg cgtgcgcccc agagcatgaa
     6121 gccgggaatg cggccggtac tgccgtcggc gctctctttc accgtattgg cgttatccgc
     6181 aaactggctg gactggaaat cgctgttcag attgaacgtc cagtttcctg gcttgtagtc
     6241 cacgcccagc gtgcctttat gtttcgggga gaatggtacc agattgccgt aggtgtcgcc
     6301 tttctcgcgg atttccgcgt tcacatacgc atagctggcg tagatggaaa cgttatcaag
     6361 cgttggcgtt agcgtaccca gatcgtaacg tgcctgcgtt tccagcccgg tatggcgcgt
     6421 tttgccacgt gcagtgacgg tgtcgttggt ctggttggag tcgtactgat tgttaaagtt
     6481 aatcaggaac agccccattt ccgccgtcag cgcgccgtcg tcgtagcggg taccgagttc
     6541 ccaggttcgc gctttttccg gttccacatt gccgctttgc acagccttgc caatctggct
     6601 gtactgtacg gtgccgaacg agccttcagt gtttgcataa agattccagc tgtcagtcag
     6661 gtgatagagc acgttcaacg ccggaagcgg tgcgttatag ctcacttctt cgtgcgtgcc
     6721 tgtgatggcg ttgttctggt atgactcgat atgttcgaaa cgcatacccg gcgtgatggt
     6781 ccagttgccg atgtcgattt tgtcatccag ataccaggcg tgcgcctcgg tgccggaacg
     6841 cgtatcgcgg tcgtaagggc ttgagccgga cggcaactgc ccgctgctgg tggcggtgta
     6901 gtaacgcatt tcatgcgttg attcattcaa atagcgatag cccacgccca cttcgtgcgc
     6961 ggaagggccg atcataaaga tctggctgta gcgtggctca ataccgcgca cccagtagtt
     7021 acgcggcgag agggtgatgc gtttgccttg ctccaggtag ccgctgcgca gggtttgggt
     7081 gtagaacccc tgaatgttga atttatgctg gctgtctggc tggaactggt agcccaggct
     7141 cgccagcttg cgacgacccc agaagcggtc atacgggcgg gtggattgcc agcgatcggc
     7201 gtcgtaatcc gcgcgagaca ggccaccggg catgtcggct tcaccatcgt aatattgcag
     7261 caggctgttg aaggtgtgca cctcatccgg cgcatatttg cttttcagca tcaggtcgtc
     7321 gatgcgggtg gcgctgtgct cgcgccagtc actgccgcgc gtgccggagt agagcagcgc
     7381 ggtgccaaaa ccgttgtccg ctgtgccgcc caccatcagg ttgtgcgtct ctttcgggtt
     7441 gttttgtgaa gaggttgggc tgagctgacc ttccacgccc gcctcgatac caaagtcctg
     7501 cggaatggca cgggtaacaa agttcaccac gccgcccacg ctctgcggtc cgtaacgcac
     7561 cgcaccacca ccgcgtacca cgtcaatggc atccatgttg ccgagcgaaa cgggagccag
     7621 tgaaagctgc ggctgaccgt aaggggcgaa ggggacgggg atgccgtcca tcaggacggt
     7681 cgagcggctg gcgaggcgcg ggctcaggcc ccggatgcca aagttcatcg ccaggtcgtg
     7741 gctgccggtg ccgttgtttt ccggcgcgct gacgccaggg atgcggttaa gtacctcacg
     7801 catggtggtt gcgccggttt tggcgaaatc ctcacggcgg atcacgtcac gcgcgccagc
     7861 atgttcaaat acgtcgtttt cacgcgcatc acccagccag tcgccgacca cggtcagggc
     7921 atcttctttt ggtgcgggcg cgggctccag cgtccagctg ttatttccca gcggttttac
     7981 ctgcagtccg ctgccgtcca gcagttgttg caggccgctc tcgacgtcgt aatcgccgtg
     8041 caggccgttg ctctgcttgc cgcgcgtcag gctggcgtca accgagaggg taaatccgct
     8101 gtgtgcggca tactgattga gcgctttatc gagcgatccc ggtgcgatat taacctgtgc
     8161 agcaaaagcg gaaaacgaga gaccggccag cggcagcagg ctcaggcgaa tggcgttaac
     8221 caaaggtgtt gttttacgaa aaacgcgtaa cggcgtcata ccttccccat catcattttt
     8281 gttgtgttca gctatgagtc gaacgagaag ggtaaaaagg acaatcgaaa taagaattat
     8341 tttccttaca gtggtgaaat gtttatccag taccgcgtaa tagactgaat tttaacggga
     8401 agcgtttgcg cgataacgtt caggatcgca tcggtatttt tcagcgggaa cgtcccgctc
     8461 aggcgcagcc cggcaacggc gggatcgcag cgcagcacgc cgttgcggta acgggttagc
     8521 gtggctatca cctcacccag cggtttatcg ctgaagctca ggatgtcctt cgtccagctt
     8581 gtactctcgt catccagcgg tttcactgcg ccaaactcag aggcgctgaa ctgcaggctt
     8641 tcaccagcgt tcacgatgcg tttttgcgcg ggggcactgg cgagaagcac ttccacagcg
     8701 tgctgctgca cgtcaagctg cgtgaaatta tcctgctggc ggacggtaaa ttctgtccct
     8761 aaagcagtga gctggccctg acgggtcagg acacggaagg ggcgttgcag ggcatctttc
     8821 gcggtagtaa tggcgatttc accgtaccag agccggacgg tgcgctgatg cgcatcaaaa
     8881 cgcacatccg ccgcgctttg ggtattcagc gtgagcaggg agccatcttc cagttgctga
     8941 cggctgactg tgcctttggc ggtgcggtaa tctgcccgca gaccttcgcc ggtttccgac
     9001 tgccagagct gccagcctcc gccagcgccg agcaacagca gtaatccttt catcacgtga
     9061 cggcgggtga ggcgggtatc gtgcaacgcc ctgctggcta cgtcgccagg cacaccgcca
     9121 agctggttgc gcaggttttc aacctgctgc caggcccact ggttatcctg atcctgttca
     9181 taccactgtt gccagcgcgc ttcctgttgt gggctgacgc gctcgccgct tagcacggca
     9241 taccagtggg aagctgaacg cagcgcctga cggcgggaat cggttaacaa aggattcata
     9301 acccatactc cagacggaac agcaggcagt gctcgacggc tttcgccacg tattttttca
     9361 cggagctgat ggaaacaccg agtttgtgcg caatctcgct gtatgtcaga ccatccagtt
     9421 gcgaaagcag aaacgcttca cgtgttttgc cgtttagccc gtccagcatg ctgtcgagga
     9481 gttgtagggt ctcgagttgg ctttcgcgtt cctcaggtga aggcgctccc ccctccggca
     9541 taagcgccag catctccaga tacgcttttt ccagcgcgtt tcggcgaaac aggtccacca
     9601 tcacgcgttt ggcgatagtg cagaggaagg agcgaggatc gcggatcgtc gagagcgttt
     9661 cgctgaccat tacccgcaaa aaagtgtcct gggcaatgtc atctgcatca aaagcagact
     9721 ggagtttgcg cgtcagccag cttttcaacc agccgtgatg tgtgccataa agcgactcga
     9781 acgttaagga agctgtggta gtggcgcggt cagacatgcg gagtgcatca aaagttaatt
     9841 atcacgtagt catattaata tgagaatggt tatcattaca attggaaata aaattgtttc
     9901 caatagacat ttttaacatg ttgtttttct aagtgttata aggtaggtat aaaatgggat
     9961 ggagcctctg cttctggcat gtgtcggtca gaatgactca tgatgtggtc tgctattatt
    10021 gacatcctca ctgccctaaa ggatgggga
//
LOCUS       DAZFJF010000151         9982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFJF010000151 DAZFJF010000000
VERSION     DAZFJF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.152"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHKAU_004265"
     CDS             <1..480
                     /locus_tag="ACHKAU_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO5533218.1"
                     /translation="GLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAAPVL
                     LDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTEAWQ
                     KLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYGLW"
     gene            467..1816
                     /locus_tag="ACHKAU_004266"
     CDS             467..1816
                     /locus_tag="ACHKAU_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO5533219.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLTEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            1800..2924
                     /locus_tag="ACHKAU_004267"
     CDS             1800..2924
                     /locus_tag="ACHKAU_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO5533220.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            2914..3528
                     /locus_tag="ACHKAU_004268"
     CDS             2914..3528
                     /locus_tag="ACHKAU_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5533221.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            3521..3958
                     /locus_tag="ACHKAU_004269"
     CDS             3521..3958
                     /locus_tag="ACHKAU_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO5533222.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            3958..5040
                     /locus_tag="ACHKAU_004270"
     CDS             3958..5040
                     /locus_tag="ACHKAU_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5533223.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            5031..5591
                     /locus_tag="ACHKAU_004271"
     CDS             5031..5591
                     /locus_tag="ACHKAU_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO5533224.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHKEENI"
     gene            5591..6487
                     /locus_tag="ACHKAU_004272"
     CDS             5591..6487
                     /locus_tag="ACHKAU_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5533225.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRDTVNRAANALPSNGTAVAASRLANARRINGVAFDGTQD
                     INITSGMPESTANQRYVTGVRLGAQALSGGLEYNYSLSSGNVITGFKTDGDWEMRGGD
                     DRVYYRQIQYCINGHWVSAASI"
     gene            6609..7130
                     /locus_tag="ACHKAU_004273"
     CDS             6609..7130
                     /locus_tag="ACHKAU_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5533226.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(7165..7551)
                     /locus_tag="ACHKAU_004274"
                     /pseudo
     CDS             complement(7165..7551)
                     /locus_tag="ACHKAU_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            7614..8174
                     /locus_tag="ACHKAU_004275"
     CDS             7614..8174
                     /locus_tag="ACHKAU_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5533227.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            8274..>9982
                     /locus_tag="ACHKAU_004276"
     CDS             8274..>9982
                     /locus_tag="ACHKAU_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5533228.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGVNVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSAWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMALYWPVSSLPQGVTLTGDA
                     GNNL"
BASE COUNT         2329 a         2420 c         2939 g         2294 t
ORIGIN      
        1 gggttacttt cgtctgtcgg tagccgcatg gggctggggc gtttctccgg ctttttccgc
       61 agtgccggtg gactggccgg ccggctgggt ggaggtgccc tgtgggctgg cgcgatggca
      121 gccccggttc tgctggatgg cagcgccagt gccacagaca agggagaagc ggtcggctct
      181 cttgcaggca gtattgccgg tggtgcgctg ggggcggcag ccggacctgt ggggattgcc
      241 attggttcta cggtgggcag ctatctcggt aactatctgg ggggatggct gactgaagcc
      301 tggcagaaat tacggggcag tgatgatgaa agcagtggac aggcggtaca aaaagcctct
      361 gcccgtgtgg agctggttgc cccggaaggg tggcaggctc gcagtattga tattgatgac
      421 acgtccggtc acggactgga tgtgaatgtc tggaacggag ggaattatgg cctctggtga
      481 cggtcgtggt gcgttccgtg gtgtgccgtt cctcgtctgg cgtgagcagc gcgaacgtgg
      541 tgggcgaaac attgtccgtc gggaatatcc gttacgggaa accggtgggg cagacgatct
      601 gggacctaaa ctgactgaat ttacgttcag tgtacttgtg atgggggatg atgttcagac
      661 acagcgaaat cgtctgcgtg atgcccttcg tgctcccggt gccggggagc tgcttcaccc
      721 ggattacggc acgttaaatg tgctgataaa cagctttgaa agtcgttata acgccgctga
      781 acagggcgtg gttgagttca ccattaatgt cacgccggta agtgatgata ccgcacctgc
      841 tgtcacacag gacacggccg ccattctgga gcagaagagt accaccgcgc tcgggcaggt
      901 ttttgaaacc ctggaagcgg gctggacggt gatctctgac ggaatgcacg atgtccaggc
      961 aatgaccgaa accatcagtg ataaggtgtc cgccctggaa aatgccgttt ccggcatggg
     1021 gattgtacag gatatcagtg cctttactgc cacgttcacg gcgctgaaag ggaatgccac
     1081 ggcattactg acagcacctt cccgcatggc ctcatctttt gcggggcttt tcagtgccct
     1141 gatcaccctg ccatcgctgc cgtcactgtc ttcaggaggc ctgaataccc ggcccggtgg
     1201 cagtatcggc cggacgacat ccgccgtctc tcagggaatg ccgcaactgt acaggacatt
     1261 atcttccctg cgttacgtgc tggatgagca ggataatccg cagactctca tcggtctgac
     1321 accggcagca caaaagaata ttcgtctgat acgggcggta atgcagagtg ctgccgtggt
     1381 ggcccaggca cagaccgtgg gaaaactgct ggatcaggtt ctcagtcagg aaaccctgcc
     1441 ggacagtgac gcagcccacc gtacctggcc tgtctggctg gaaagttcag ttgatcttca
     1501 gcgcattaac cgtgacttaa gcgaagcgct cgaacggcag gtgatgacac tgtccgggca
     1561 ggggtatacc gccacggcgc tgacgcttcg tgatgccaga ctggcactga cagaggatct
     1621 gaatacacgg ggagttcagc tacccggtgc aacagtagtg actgtacgta ccaccgagcc
     1681 tgcactggtg accctgtacc gtgccaccgg gaacagtacc ggctggcaac gttttgtgcg
     1741 ccgtaacggt attgttgatc cgctgttcat tcccggaggc cattcagtgg aggtgattag
     1801 tgagcagcag ggttgaactg tatctgggcg gtgagatttt ttccggctgg ctgacggtga
     1861 gtgttcgtcg ctctcttgaa catctggcgg gctcctttga actgggggta atgatgcccg
     1921 gtgtacgcct tccgtcatcc gtccgtgccg gtcagtctct ggaattgcgc attgacggtc
     1981 agcctgtgat cactggctgg ctggatcagg tccggcagcg catcagcgcc acgcgttttc
     2041 agatcacgct cagcggacgg gataaaaccg gtgacctggt ggactgttca gccattcatc
     2101 cgggcagcca gtggaggaac cgcacgctgg agcacattgc ttcagatttg tgtgctccgt
     2161 tcggggtcac ggtgcgctgg caggtaaatg atgcaacggc agcccggccc ttttccacct
     2221 tcacactgga aaattcagaa accgtggcag atgcgctgac acgggccgcg agacacagag
     2281 gcgtcctggt aaccagtaat gccgccggtg aactggtttt cactcaggcc ggcagtcagc
     2341 gtggcgacac gctgacgctg ggcgaaaatc tgctggattt ggatcacaac gtggatcacc
     2401 gtctgcgcca cagtgaatac cgtgtacggg ggcacgggcg tggtggtggt catgccgggg
     2461 atgcactgac agccggaacg ctggccgcac cagttggtac ggtgacagac agtgccatcc
     2521 accgttacag accgaaaatt gtgctggcgg atcatgctgt tgatgcagac ggtgcacgcc
     2581 agagggctgt ccgggaaatg cgccgggcgg ttgcccgctc tgtgcgcctg acagccaccg
     2641 tgcggcactg gtttcgggag aacggccagt tgtgggatat caacctgctg acggctgtca
     2701 cggctccccg caccggagtg gaagagcgtg atcttcttgt ctgtcaggtg gagttttcgc
     2761 tggatgcaaa tcacggcgaa accacccgtc tgattctggc accccgtgac ggctttattg
     2821 ttccggcaga gccgggaaac agcggaagcg gaaatgcggg tgacgtggac gccttcgtgc
     2881 gggcacagat gaaaaaacag gggattaaat tcaatgatga atgacgaagt catcagccgc
     2941 cttctggccc ccgtgatgcg gggtgttcgt ctgctgttcg ggcgtggtgt actgaccggc
     3001 acaacggaca cgctgaaaat ccagaatgtg caaatcaccg gtatggatgg tgaaaccttt
     3061 gatgacgttg aacgccccca gcagtacggg caaatcagcg tccccctgcc tggtgcggaa
     3121 gtttttctgg cctgtgctgg cggacaacgg gatcaggccg tggtgcttgt ggtggaagac
     3181 cgccgcagtc gcccgaccgg acttaccgcc ggagataccg gcgtgtatca ccatgagggg
     3241 caccgtatac gtctgacaaa gaacggccgg atcattgtga cctgtaagac actggagatt
     3301 tacgccgatg aaggtgttca ggtggataca ccggaggctc actttaccgg taatatcaca
     3361 gtggataaga acctgcatgt caaaggcaat gtgtccattg acggcaccgg aagatcacag
     3421 ggggcgttca cgatgtccga agcggttatt gccgggatca cctattcagg tcatgtgcat
     3481 cacgataacg gtgaaggcag caaaacggga ggaccagaga atggctgata ttgctgtcgt
     3541 ctgggatcag ggttgtggtt ccctgcaact gaacggcgca gatcttctga cggataacag
     3601 cctgctgact gcggtcatta tttcactgtt tacggacagg cgggcgctgg attccgatga
     3661 aatccctgac ggcacccgtg accgtcgggg atggtgggga gacagtttcc gggagcgccc
     3721 cattggctcc cgtctctggc tgttaagccg tgaaaagacg ctgtcctccg tggtcagccg
     3781 tgcacaggcc tatgctgatg aagcgctggc gtggctgcat aaaagcggtg ctgccacatc
     3841 cgtggtatgt catgccatgc gtgtggggca tgatcgcctt tcgctttccg tgaaaatcac
     3901 cctgccggac ggaagcagac atccgatgat tttttatgct gatatgaagg gggaatgatg
     3961 ccttatcagc ccttaccact ggcgcaactg atcacacaga cacaacagga tatcagtcag
     4021 cgcctgcccg gttcgcagcc gggcgtgaat gaaaccaccc tgaatgccat tgcgtatgca
     4081 ctggcgggtc tgtcagcaca ggagcatgaa catctggcct ggatctcccg gcagataatt
     4141 ccgacagaag ctgatgaagc cgaactcctg aaacactgcg cattctgggg tgtcatccgt
     4201 aaaccggctt cccgcgctga cggaccggta caactgatgc tgaccacgga tgcagggatc
     4261 acggaaggcg tactccttca gcgaagcgat ggtgttgtgt accgcatcac cggctctgcg
     4321 accggaaaag ccggaacact gaatgttaat gtggaggcgg aaagtgcggg gcgcgccgga
     4381 aataccccga ccggaacccg cctgtccttt atcacgccac aggcgggcat caaccagaca
     4441 gccacggtta ccggcacggg actcaccggt ggtgcggatg tggaaacggt gccggagttg
     4501 ctgtccaggc tggtattccg ggtacagaac ccgccatcag ggggaacaca gtatgatttt
     4561 gaacgctggg cacgggaagt accgggcgtg acgcgggcat ggtgtaagcc tgaatggcct
     4621 gaggcgggta gtgttggtgt gacttttgtt caggataata accctgacat tttccccgga
     4681 gaaggtgatg tgaagcgggt ggcggattat atccgcagtc atgatgatcc ggcgacgggc
     4741 cagcccgttg gtcagccact tgggccgaca atcagcgtgt ttaagctgac caataagccg
     4801 gtggcgtttg agatcaggat tgtacccaaa acgccggaaa atcaggctgc cgtaaaacag
     4861 gcattaacgg acctgcttta taacgaatcg cggccgggtg gacttgtatt gccttcatca
     4921 ttctggcggg ctgttgcagg ggtgaaagga ctggaggatt ttgaagttcg cagtccgctg
     4981 aagtccgtga tggccggaga tacagagttg ctgaccgtgg gggaaatcac atggctgtaa
     5041 ccctgacccc gcatcagcgc gccctgttgc agttgctgcc tgacgggctg gcatgggata
     5101 agcggccgtc atccgttctt gcggctttgt gcctgggcct cagtcattcc acggagcgtg
     5161 tttcctggac cggtaaccag atgctggcag aacgttttcc tgattcatcc cgtctgctgc
     5221 tggaagactg ggagcgttat ctggggttac cggaatgtga tatgaccggc gcaaccattc
     5281 aggaacgtca gcgttatgca gggaataaat accggatgaa accctctctt aaccgtgaat
     5341 tttatatccg gtttgcggca gagtttggtt atgaaataga tattcagcca tcaccggatt
     5401 cacagtgggt cagtattgtc acgataaaca gtgaaaccgg ctaccggaat atgaatgtgc
     5461 tggatgatat tctcaccccg ctgcgtattt atgagggtgg tgcgctggaa tgtattctga
     5521 accgttataa gcctgcatgg cagacgttta tttacgtgta tgcaaacagt cacaaagagg
     5581 agaatattta atgttccatg ttgataataa ttccggcgtg gcgaatatgc ctgaactggc
     5641 tccggcgcag agtaatacca ccacctggtt taccgaaggt gacggacaaa aaggtatcag
     5701 ctggattggt caggactggc tgaatattct ccaggccgaa ctgctgaata ttctggctga
     5761 agccagtatt cagccggata aggcgcagtt aaaccagctt acgctgtcca ttaaagccat
     5821 tatcgctgcg aatgcctttt cccggaaaaa caacctgaaa gaaattgctg atgccggtgc
     5881 ggaagcccag cgtctggccc gtggttatct tggtctgggg acgcttgcca caaaaaacag
     5941 tcttggtccc gctgacgtta atgccctggc gaaggatcag aaccttgccg acctggagaa
     6001 tgcggaaacc gcccgtaata atctggatgt ttacagcagg agcgaaggtg ataaccgtta
     6061 cctgcgcagg gaccagaatg gcgcggatat ccaggacaaa ggaaccttta tcgataacct
     6121 cggtttacgg gatacggtaa acagggccgc gaatgccctg ccatcaaacg gtacggctgt
     6181 tgcagcaagc aggctggcaa atgcccgcag aatcaatggt gtcgcctttg acggcacgca
     6241 ggatataaac attacgtcag gaatgccgga atcaacggcc aatcagcgtt atgttacagg
     6301 ggtaagactg ggagcgcagg cattaagcgg cggactggaa tataattact ccctgtcatc
     6361 aggaaatgtg attaccggtt ttaagactga cggagactgg gaaatgcgtg gtggtgatga
     6421 ccgtgtttat tacagacaga tacaatattg tattaatggg cattgggtat ccgctgcaag
     6481 tatttaatta acgggggcat agcacacagc agccagcatc tgaaatatgc gttatttcag
     6541 acaagcattg cctgtatatg gcgatagtat tattcctgtt tttatcattg atttacatgg
     6601 tgatgtttat gcaactgatt aatgtcaaac gatattaccc gaaagacaaa ccctatggtg
     6661 aggatgttca gtatttccag agtgaggacg gaagggattt ttatgaatct atcccgcttt
     6721 ttacaaagaa atacaaactc tgtatttcgc ctgtaacggg tattatctgc tctgttgctg
     6781 aagatgtctc tgctttatat ccggcaggtt ttactgttgt tgaagtggac gaactgccgg
     6841 aaggtgtgaa tattgacggt aactggcagt tttctgatgg cctgatatca aaggtgccgg
     6901 taaactggaa gacggttgct gaaaagcgtc gctcatcgct gctccaggaa gccaacgaaa
     6961 ccgtggatga ctggaaaaca gaattaaaac tggacatgat tagcgatgaa aataaattac
     7021 agctgacgcg ctggatggcg tatatccgac agcttaaaga aatgcacttt aatgatattg
     7081 ccagtgaggg gcattaccag gcgattccct ggccggaaaa accggaataa ttcagagggt
     7141 gcattcagca cccttttctg tggtttacac tgaacttacc gttctccagg tattattaat
     7201 ataaatctga accgggcgat aatagacccc accgatattg tccgcagacc gggagccggt
     7261 ttcctgcacg ttaataccgg aaagcataca accggaaggt gcgctgtaat tccatgacat
     7321 ttcattgcct ccgggtgaat gatagctctg tgctccaagc tgaacattct gtacataccg
     7381 gccatctgcg gttgactgcg tcattcctga cgtgatgttg atatccagcg ttccgtcaaa
     7441 aggaacacca ttaatatttc ttggggtggc gagtctgttt gctgccacgg cggttccagc
     7501 cgatggcagg gcatcagcag ccttatttac cgtttcccgt aaaccgaggt ttcagggatg
     7561 ccgtatatct actttaaaag gcttttccac ctctttttaa ggaaggtaaa tacatgctga
     7621 ttggctatat acgcgtatca acaaatgacc agaacaccgg tttacaaaga aatgcgctgg
     7681 aatgtgcagg atgtgagcag atttttgaag acaaaatgag tggtaaaacg acacagcggc
     7741 cgggactgaa gcgggcactg aagaaactaa aacccggtga cacgctgatg gtctggaaac
     7801 tggacaggct ggggcgcagt atgcgccacc ttgttgcgct gacagatgaa ttacagcaac
     7861 aggggataaa ttttcgtagt cttactgaca acattgatac gtcaaccccc atggggcgct
     7921 tcttttttca cataatgggt gcgctggcag aaatggagcg tgaattaatt gtcgaacgga
     7981 cacgggctgg tcttgcagct gcccgtgaaa ggggacgaac cggcggcaga ccccgccgtc
     8041 tcacgcagga acaatacgag caggtcgggc gtttactggc cgggggtgtc agtcgaagac
     8101 aggtggcact tatttttgat atcggtcttt caacccttta tcgttattat cctgcgagcg
     8161 tttcggaaaa ataagacgct ctctttgttg ggcctttact gaattaacgc aaaatggcgt
     8221 ttatcggtct gtggatatga taattcagtt tattcaggaa caggctgatt attatgacgt
     8281 ccaaatgggt tcaactatca tcaatgcccg gaaactttac tgttaaagtt tccggtggta
     8341 cagcggcatt tcttgaggct ccctttcctc cggccgaaac aaaaggagga atgacatttg
     8401 ctgactgtct catcagtttt aatacacggg attgcctgtg ggtaaggcca gtatccggtg
     8461 atccgagtgt ggaaattacc ggagcgggta ttggtgcggt cattccgtta agtgctgatg
     8521 ttgccggtac tgctgaaccg tcagactggg ataatgctga aacacatacc cgtccgtcag
     8581 gaaatgaaac tgcttccagc tccccttcct ggtattacgt ggtggttctt gctggtcagt
     8641 caaacggtat ggcttacggt gaaggtctgc cactgccgga gacatatgac cgccccgaac
     8701 cgcgtattat gcagttagcc cgtcgcagta cagtcacgcc gggaggtaaa gcctgtcagt
     8761 ataacgacat cattctggcc gaccactgtc tgcatgatgt gcaggatatg agtggaaaaa
     8821 atcacccgaa agcggatgtg gctaaagggc aatatggcac cgtgggtcag gggcttcaca
     8881 ttgccaaaaa actgctgccg tttattccgg cagatgcggg gattctgctg gttccctgct
     8941 gccgtggtgg ttctgcattc acggcgggtg ctgacggcac gtacagtgac agcacgggtg
     9001 cttcagagga ctcagcccgc tggggtgtgg ataagccgct gtataaggac ttaatcagcc
     9061 ggacaaaagc ggcactggcg aagaacccga aaaaccgcct gcttgctgtg gtgtggatgc
     9121 agggcgagtt tgatattgat gcgaagccga cggagcattc tgcgctgttt ctggcgatgg
     9181 tggaaaaatt ccgcgcagac ctggctgaac aggcggaaca gtgtaccggt ggcagtgctg
     9241 ctggcgttcc gtggatttgt ggcgatacca cgtatttctg gaaacagaaa aatgaaccag
     9301 cataccaggc aatctatggc ggctacaaaa acaaaacaga taaaaacatt catttcgttc
     9361 cattaatgac agatgaaaat ggcgtgaatg tacccaccaa caatccggca gaagacccgg
     9421 atattgaatc cattggttat tacggctcaa cgtggcgtaa cagtgccgct acctggacat
     9481 ctgcggaccg ggccagccat ttcagtgcct gggcgcgccg gggtattatt tctgaccgtc
     9541 tggccacggc tgttctgact catgcagggc gaaccacagt aaaagccgat gttccatctt
     9601 ctgaaactga agcaccagtg ccgtcacctt cagaaactga agcagtaacc acaacactgc
     9661 tgtcttaccg tgtcagtgag tcagaaggaa atctgaaagc ccagggctgg gagccggctg
     9721 gcggtaaagc tgaaattatc agtgatgcag gaggcacagg tggtaaggca atgaaactga
     9781 ccaaggaaac aggtaaatca tcctggtatc tggatcatga tgccggtact ggtgctgaac
     9841 tgctgaaaaa tggtggttta atcagttgtc gttttaaagt acctggcgac ctggtggcaa
     9901 accagtatgt catggcgctt tactggccgg tttcctctct gccgcagggt gtcaccctga
     9961 caggggatgc agggaataat ct
//
LOCUS       DAZFJF010000152         9892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFJF010000152 DAZFJF010000000
VERSION     DAZFJF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.153"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(400..1593)
                     /locus_tag="ACHKAU_004277"
     CDS             complement(400..1593)
                     /locus_tag="ACHKAU_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5533229.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1729..3453
                     /gene="iucA"
                     /locus_tag="ACHKAU_004278"
     CDS             1729..3453
                     /gene="iucA"
                     /locus_tag="ACHKAU_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO5533230.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3454..4401
                     /gene="iucB"
                     /locus_tag="ACHKAU_004279"
     CDS             3454..4401
                     /gene="iucB"
                     /locus_tag="ACHKAU_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO5533231.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4401..6143
                     /gene="iucC"
                     /locus_tag="ACHKAU_004280"
     CDS             4401..6143
                     /gene="iucC"
                     /locus_tag="ACHKAU_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO5533232.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6140..7477
                     /gene="iucD"
                     /locus_tag="ACHKAU_004281"
     CDS             6140..7477
                     /gene="iucD"
                     /locus_tag="ACHKAU_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFO5533233.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            7483..9678
                     /gene="iutA"
                     /locus_tag="ACHKAU_004282"
     CDS             7483..9678
                     /gene="iutA"
                     /locus_tag="ACHKAU_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO5533234.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2339 a         2580 c         2625 g         2348 t
ORIGIN      
        1 tccagctctt tcaggtgtca acgccactct gagagagttt tatgcatccc gtattgggca
       61 ctatgatgcc ggaaatctgc gaggctcgac acctctctga ttttcccctt tatttatcac
      121 ggtgccccag atctaatgtt ggggatgcgt tcatctcatt cccaatgagt aatcgcactg
      181 ttctcattgt ctgcaggcat ccggacagag ccatgtgatg ttgcactcac aaccagcatg
      241 acttcaatgc aacttaagtg acaccggccg gttttatacc gaacataccc tgtaccatgc
      301 ggtgcatttt tgccagtgtt actgcctgac atgctttcat cagacgcagg ttctctctca
      361 tatcctctga gttactccgg taaggtgatg atcatatctt tatttgatag cacggtaatg
      421 ccagataaat acagacacaa tggtaaataa tatagttgca aataatgccg ccataatcag
      481 cgcgattgcg acacctgttt tcgataccag cggcaccagc aaagcgcaca gtccatagcc
      541 aagtgtgtga ctggtagcga gccagcccgc gcctgcgcca tcagccagct tatcattcag
      601 tagaagttga taagcgggtg ttgccagagc tgctccaaat gacagcactg cacagcccgg
      661 gtaaaacaac catagctgtt cggagagcat gatagccaga ccaccactca tcaacactcc
      721 ggcactcagg agcagggcca ccggagtcag gcgctgcgga cggagtaccc cgaactgcgc
      781 gataagcgca gctactgcgg acagacccaa cagccaggcc acctgttggc taatggcggt
      841 ggtatccgtg gcgaactggc gagtaagggc aggcgaaagt ccaagctgca tcatgctcac
      901 cgccgcagcc agtaatattg cgcaaagcag ataaggcaga caatcccgct tcagactgac
      961 gctcttgcgc tccggtgtgg gctgtggtgg tgtgccgggc aaccgcagca gcatcagcag
     1021 cgccagcact ggggctgcca tcagcagccc cagtggcgcc agaggatgaa tggccagcat
     1081 tgctgccgcg cacagcggcc cgaatagcct gccgcaactc aggccggagc tgatggttgc
     1141 cagagcggcc atgcgattcc cttctcctgc tctctgcaat gcccagacct gacaggctgg
     1201 caccattgcc gacacggtca gcccgtaggc gatccgggcg atgattaata ttcccagtcc
     1261 caccgctgtt gttatggcgc tggtagccat cagcacgctt cccagcccta gcagggtaaa
     1321 gcttatgcca tatcctccca gagcccacaa caccacaggt ttactgccag tacgggaaat
     1381 ttgctttccc caccacgggg acgatggcag aaacagcatt gagccaagca tcagcagggc
     1441 agcccataca gacagactca gatttgtctg tatgaccaga acagggagca caaccagcag
     1501 accgttctgc ccgataccga gaagcccggc actgaacgca agtggccagc aggacagtgg
     1561 tttttggggc gtatcttcga tcccaggtga cattttaatg tttcaactcc atgtattaat
     1621 tgtgtttatt tgtaaaatta atttatctga caataacatt tcttattgat aatgagaatc
     1681 attattgaca taattgttat tattttactg tgtgggagct gtttgactat gaccctgccc
     1741 tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc tgaatgcact gattcgtgaa
     1801 actacagact ggaaactgac agaatacccg ccagacgaat tgatcatccc gctggatgag
     1861 cagaaatcgc tccatttcag agtggcttat ttctccccaa cccagcatca ccgctttgca
     1921 tttcctgcac gtctggtcac ggcatcaggc agttatcctg tcgactttac cactctctcc
     1981 cggcttatta ttgataagct acggcatcaa ctgtttctgc ccgttcccct ctgcgaaact
     2041 ttccaccagc gcgtgctgga aagccacgcc catacgcaac aggcaattga tgcccgtcat
     2101 gactggaccg ccctgcgtga aaaagcgttg aattttggcg aagctgagca ggcgctgctg
     2161 acaggacacg ctttccaccc tgcgcctaag tctcatgaac cgtttaaccg gcgggaggct
     2221 gaacgctacc tgcctgatat ggcaccccac ttcccactgc ggtggttttc ggtggataaa
     2281 acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac agcggctgac gcgatttgcc
     2341 gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca accaatggct gttcccgttg
     2401 cacccgtggc agggggaata tcttttgcag caggggtggt gccaggcact tgttgctaag
     2461 gggctgatta aagacttagg tgaggccggc acgtcgtggc tgccgaccac ctcttcccgt
     2521 tccctctact gtgccaccag ccgcgatatg atcaagttct ccctgagcgt acggctgacc
     2581 aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg ggatgcgcct ggcacgcctg
     2641 gctcaaaccg acggctggca gatgctacag gtccgcttcc cgactttccg ggtaatgcag
     2701 gaggatggct gggccgggct gctcgatctt aacggcaaca tcatgcagga aagtctgttt
     2761 gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc agaccaacgt actggtctcc
     2821 ctgactcagg ccgcacctga tggcggtgat tcgctgctgg tttcggcggt aaaacgcctg
     2881 agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg catgggtcga tgcatactgt
     2941 cagcaagtgc taaagccgct gtttacggct gaagcggatt acggcctggt gctgctggcg
     3001 catcagcaaa atattcttgt ccagatgctt ggggatctgc cggtcggatt tatttaccgt
     3061 gactgtcagg gcagcgcttt tatgcctcac gcgacagact ggctcgattc tattggcgag
     3121 gcgcaggcgg aaaatatttt cacccatgag cagttgctgc gctatttccc ttattacctg
     3181 ctggttaact ccacttttgc tgtgaccgct gcgctggggg ctgccgggct ggacagcgaa
     3241 tcgaatctga tggctcgtgt acgagcatcg ctggctgaag tgcgtgatca ggtgactcat
     3301 aaaacctgtc tcaactacgt gctggaaagt ccgtactgga acgtaaaagg taactttttc
     3361 tgttatctga acgatcataa cgagaacacc attgttgacc cttcggtgat ctatttcgat
     3421 ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg aggcaaacat tattcacagc
     3481 agatatggac tgcgctgtga aaaactcgac aagcccctga atcttggctg gggactggac
     3541 aatagcgcgg tgttgcactg tcccggggag ctgccgacag ggtggctgtg cgatgcgctg
     3601 gatcagatat ttatcgccgc accacaactg tcagcagtgg ctctcccctg ggccgaatgg
     3661 tgtgaggagc cacaggcgct gacgcttttt gggcaggtaa aaagcgacat tatccatcgt
     3721 accgccttct ggcagttacc gttatggttg agttcaccgg caaaccgggc ctccggtgaa
     3781 atggtttttg atgcagagcg tgagatttat ttcccgcagc gcccaccccg tccgcagggt
     3841 gaggtttacc gccgttacga tccacgtatt cgcaggatgc tgagtttccg cattgccgat
     3901 cccgtttctg atgcagaacg tttcacccgc tggatgaatg atccgcgcgt tgagtatttc
     3961 tgggagcaaa gtggctcact ggaggtacag accgcctatc tggagcgcca gctcaccggt
     4021 aaacatgcat tcccgctgat tgggtgcttc gacgatcggc cattcagcta ttttgaaatc
     4081 tactgggcgg cggaagaccg cattggtcgt cactattcgt ggcagccctt tgaccgtggc
     4141 ctgcatctgc tggttggtga acagcaatgg cgcggggccc actatgtgca aagctggctg
     4201 cgcgggctga cacattacct gctgctggat gagccccgta cgcagcgcac agtactggag
     4261 ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata ccggacaatt
     4321 aaagagttcg acttcccaca taagcgctcg cgcatggtga tggcggatcg tcatcacttc
     4381 ttcacggagg tcggtctgta atgaatcaca aggactggga ttttgttaac cgtcggctgg
     4441 tggcaaaaat gttgtctgag atggagtatg agcaggtttt ccacgcggaa tctcagggcg
     4501 atgaccacta ctgcattaac ctgccgggag cacaatggcg ctttatcgct gaacgtggta
     4561 tctggggctg gctgtggatt gatgcccaaa cgttgcgctg cacggacgaa ccagtactgg
     4621 cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat gagtgatgcc actgttgcag
     4681 agcatatgca ggatttgtat gccacgctac tgggcgacct gcaactgctg aaggcccgtc
     4741 gcggactgag tgccagtgac ctgattaatc ttgatgccga tcgtctgcaa tgcctgctga
     4801 gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggcaaa gaggcgctgg
     4861 aacgatatgc gccagagtat gccaacacct tcagactgca ctggctggcg gtaaaacgtg
     4921 aacatatgat ctggcgctgc gacaacgatc tggatattca gcagttgttg actgccgcaa
     4981 tggatccgca ggagtttaca cgcttcagtc aggtctggca ggaaaacggg ctggatcaca
     5041 actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgcc accgacttca
     5101 tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac cagtggctcg
     5161 cccagcagtc gctgcgtacc ctgaccaacg ccagccgccg gggagggctg gatatcaagc
     5221 tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga tatatcgctg
     5281 ccggaccact ggcttcacgc tggctgcaac aggtttttgc gaccgacgcc accctggtgc
     5341 aaagcggcgc agtgatcctt ggtgaaccgg ctgcaggcta tgtgtcccat gaaggctatg
     5401 ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc tggcgggaga
     5461 atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca ctgatggagt
     5521 gcgacgaaaa caatcagccg ctggcaggcg catatatcga ccgctccgga ctggacgctg
     5581 aaacctggct tacgcaattg ttccgggtgg ttgtggttcc gctgtatcac ctgctttgcc
     5641 gctacggtgt cgcgcttatt gcacacggac aaaatataac gctcgccatg aaagaggggg
     5701 taccacagcg tgttctgctg aaagacttcc agggcgatat gcggctggtg aaagaagcgt
     5761 ttcccgaaat ggactctttg cctcaggagg tgcgtgatgt cacatcccgt ctgagtgcgg
     5821 actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt tttatttcgc
     5881 cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg gcagcagtgt
     5941 tgagtgatta catgaacaaa catccacaaa tgtcagagcg ttttgcgctt ttctcactct
     6001 tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg ccggaccagg
     6061 atggcggcag tcgcatgctg ccgaattacc ttgagaatct gcaaaatccg ctgtggctgg
     6121 taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtcg ggacagggcc
     6181 atttaatctc agtattgctg cattgtcaca tcagatcgaa gaactgaact gtctcttctt
     6241 tgacgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt gtcatatgca
     6301 gactgtcttt ctgaaagatc tggtcagtgc agttgcacct acaaatccct acagctttgt
     6361 taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac tacgtacagt
     6421 atcccgtgaa gagttttctg actatctccg ctgggctgct gaagatatga ataacctgta
     6481 tttcagtcat accgttgaaa atattgattt cgacaaaaaa agtcgattgt ttctggttca
     6541 gaccagtcgg ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa aacaacctta
     6601 tttaccaccc tgtgtgaagc atgtgacaca atcctgtttc catgccagtg aaatgaatct
     6661 tcgtcggccg gacctgagtg gaaaacggat aactgtggtt ggtggaggac agagtggtgc
     6721 agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa taaactgggt
     6781 ctcacggcgt aataatttta acgcactgga tgaggctgct tttgctgatg agtattttac
     6841 acctgaatat atttcaggct tctccggact gaaggaagat attcgccatc agttactgga
     6901 tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca tttatcgtga
     6961 gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc tacccagccg
     7021 ctcggtaaca actctggaaa gtagtggtcc gggctggaag ttattgatgg aacatcatct
     7081 ggatcggggc agagagagcc tggaaagtga tgtggtgatt ttcgccacag gttaccgttc
     7141 tgcattgcca caaatacttc cctcactgat gcccttgatc accatgcacg ataagaacac
     7201 ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaaa ataacatctt
     7261 tgcggtcaac gccagcatgc aaactcatgg catcgccgaa ccccagctca gcctgatggc
     7321 ctggagatct gcacgtattc ttaatcgcgt actggggcgt gatttattcg atctcagtat
     7381 gccgcccgca ctgattcagt ggcgcagcgg cagccggaaa aaaccgcagc cggaggctgc
     7441 tgctttaact cactatacaa caaatattca ggaataacga tgatgcgcaa aaagtatatg
     7501 ccccgggctc ttggtccgct gcttcttgtc gtgctgtcac cagctgtcgc ccagcaaaac
     7561 gatgataatg agatcatagt gtctgccagc cgcagcaatc gaactgtagc ggagatggcg
     7621 caaaccacct gggttatcga aaatgccgaa ctggagcagc agattcaggg cggtaaagag
     7681 ctgaaagacg cactggctca gttaatcccc ggccttgatg tcagcagcca gagccgaacc
     7741 aactacggta tgaacatgcg tggccgcccg ctggttgtcc tgattgacgg tgtgcgcctc
     7801 aactcttcac gttccgacag ccgacaactg gactctgtcg atccttttaa tatcgaccat
     7861 attgaagtga tctccggcgc gacggccctg tacggtggcg ggagtaccgg agggttgatc
     7921 aacatcgtga ccaaaaaagg ccagccggaa accatgatgg agtttgaggc tggcacaaaa
     7981 agtggcttta acagcagtaa agatcacgat gagcgcattg ccggtgctgt ctccggcgga
     8041 aatgaccata tctccggacg tctttccgtg gcatatcaga aatttggcgg ctggtttgac
     8101 ggtaacggcg atgccaccct gcttgataac acccagaccg gcctgcagca ctccaatcgg
     8161 ctggacatca tgggaaccgg tacgctgaac atcgatgaat cccggcagct tcaactgata
     8221 acgcagtact ataaaagtca gggggacgac aattacgggc ttaatctcgg gaaaggcttt
     8281 tccgccatca gcgggagcag cacaccatac gtcagtaagg ggctgaattc tgaccgcatt
     8341 cccggcactg agcggcattt gatcagcctg cagtactctg acagtgattt cctgagacag
     8401 gaactggtcg gtcaggttta ctaccgcgat gagtcgttgc ggttctaccc gttcccgacg
     8461 gtaaatgcga ataaacaggc gacggctttc tcctcgtcac agcaggatac cgaccagtac
     8521 ggcatgaaac tgactctgaa cagccaactt atggacggct ggcaaatcac ctgggggctg
     8581 gatgctgagc atgagcgctt tacctccaac cagatgttct tcgatctggc tcaggcaagt
     8641 gcttccggag ggctgaacaa ccataagatt tacaccaccg ggcgctatcc gtcatatgac
     8701 atcaccaatc tggcggcctt cctgcaatcc agctatgaca ttaatgatat ttttaccgtc
     8761 agcggtggcg tacgctatca gtatactgag aacagggtag atgattttat cgactacacg
     8821 cagcaacaga agattgctgc cgggaaggcg atatctgccg acgccattcc tggtggttcg
     8881 gtagattacg ataactttct gttcaatgct ggtctgctga tgcacatcac cgaacgtcag
     8941 caggcatggt tcaatttttc ccagggggtg gcattgccgg atccggggaa atattatggt
     9001 cgcggcatct atggtgcagc agtgaacggc catcttcccc tgacaaagag cgtgaacgtc
     9061 agcgacagta agctggaagg cgtgaaagtc gattcttatg aactgggctg gcgctttacc
     9121 ggtgacaacc tgcggactca aatcgcagca tattactcgc tttccaataa gagcgtggaa
     9181 aggaataaag atctgaccat cagtgtgaag gacgacaggc gccgtattta cggcgtggaa
     9241 ggtgcggtgg actacctgat cccggatact gactggagta ccggtgtgaa cttcaatgtg
     9301 ctgaaaaccg agtcgaaagt gaacggtcaa tggcaaaaat atgacgtgaa ggaatcaagt
     9361 ccatcgaaag cgacagctta cattagctgg gcgccggaac cgtggagtct gcgtgtgcag
     9421 agcaccactt ctttcgacgt aagcgatgca gagggtaacg atattaatgg ttacactacc
     9481 gtcgatttta tcagtagttg gctgcttccg gtgggaacac tcagcttcag cgttgagaac
     9541 ctcttcgacc gtgactatac cactgtctgg ggacagcgtg cacctctgta ctacagcccg
     9601 ggttacggcc ctgcttcact gtacgactac aaaggccggg gccgaacctt tggtctgaac
     9661 tactcagtgc tgttctgacg gggattcttt tgcaacaagg gtatgctgta aacaacatgg
     9721 tccttggtgg ccatgttgtt tacagccagt ataatataca gccttaacaa gatattccgt
     9781 ctccggaatg gactgttcac cactgaataa caatatgtct gttttatgga tctttgactg
     9841 aaatttatcg catatcctca tttcattgtc cgtctgaaaa ctaaccttac aa
//
LOCUS       DAZFJF010000153         9882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFJF010000153 DAZFJF010000000
VERSION     DAZFJF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.154"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            80..1297
                     /gene="caiB"
                     /locus_tag="ACHKAU_004283"
     CDS             80..1297
                     /gene="caiB"
                     /locus_tag="ACHKAU_004283"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO5533235.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            1371..2924
                     /gene="caiC"
                     /locus_tag="ACHKAU_004284"
     CDS             1371..2924
                     /gene="caiC"
                     /locus_tag="ACHKAU_004284"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO5533236.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            3033..3818
                     /gene="caiD"
                     /locus_tag="ACHKAU_004285"
     CDS             3033..3818
                     /gene="caiD"
                     /locus_tag="ACHKAU_004285"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO5533237.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            3824..4414
                     /gene="caiE"
                     /locus_tag="ACHKAU_004286"
     CDS             3824..4414
                     /gene="caiE"
                     /locus_tag="ACHKAU_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO5533238.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(4500..4895)
                     /gene="caiF"
                     /locus_tag="ACHKAU_004287"
     CDS             complement(4500..4895)
                     /gene="caiF"
                     /locus_tag="ACHKAU_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO5533239.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(5156..8377)
                     /gene="carB"
                     /locus_tag="ACHKAU_004288"
     CDS             complement(5156..8377)
                     /gene="carB"
                     /locus_tag="ACHKAU_004288"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO5533240.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(8395..9543)
                     /gene="carA"
                     /locus_tag="ACHKAU_004289"
     CDS             complement(8395..9543)
                     /gene="carA"
                     /locus_tag="ACHKAU_004289"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO5533241.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2343 a         2603 c         2598 g         2338 t
ORIGIN      
        1 gagcacgaat gtaggccgga taaagcgttt acgccgcatc cggcagtcat gcgctacata
       61 aatttttcag gagctaataa tggatcatct acccatgccg aaattcgggc cgttggccgg
      121 attgcgcgtt gtcttctccg gtatcgaaat cgccgggccg ttcgccgggc aaatgttcgc
      181 agaatggggt gcggaagtta tctggatcga gaacgtcgcc tgggccgaca ccattcgcgt
      241 tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt taaatatttt
      301 caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata tcttcatcga
      361 agccagtaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac tgtggcagca
      421 caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca ccgaggagta
      481 caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc tgattcagaa
      541 cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact tttctggcct
      601 gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg gtaaaggcga
      661 aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact tcatgatgga
      721 ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc cctactacgc
      781 cggttgcggt ctgtataaat gcgccgacgg ctacatcgtg atggaactgg tgggcattac
      841 ccaaattgaa gagtgcttta aagatattgg cctcgcacat ctgctcggta cgccggaaat
      901 cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac tggtagaaga
      961 gaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac gctttgccga
     1021 actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca atccacagta
     1081 tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct gcaaagggcc
     1141 gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa tgccctcaca
     1201 tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg acattcagga
     1261 gttggtcagc aaaggtctgg ccaaagttga ggactaaacg cgtggcgtca gtcggcttgc
     1321 ctgactagcg cccgcccaat aacaaacgaa caaaaatgga tagaggtgca atggatatca
     1381 ttggcggaca acatctacgt caaatgtggg acgatctggc ggacgtttac ggtcataaaa
     1441 cggcgctgat ttgtgaatcc agcggcggag tcgttaaccg gtatagttat cttgagttaa
     1501 atcaggagat taaccgcacg gcaaacctgt tttatacgct ggggattcgc aaaggcgaca
     1561 aggttgcact acatctcgac aactgcccgg aatttatctt ttgctggttc gggctggcaa
     1621 aaattggcgc gattatggtg ccgattaacg cccgcctgtt acgcgaggaa agcgcgtgga
     1681 tcctgcaaaa tagccaggcg tgcctgctgg tgaccagtgc gcaattctat cctatgtatc
     1741 aacagattca gcaggaagat gccactcaat tgcggcacat ttgcctgaca gatgtggcac
     1801 ttcccgctga tgatggcgtg agttcgttta ctcaactgaa aaatcaacaa cctgccacct
     1861 tgtgctatgc accgccgcta ttgactgacg atacggcgga aattctcttc acctccggca
     1921 ccacctcccg accgaaaggt gtggtgatta cccattacaa cctgcgcttc gctggatatt
     1981 actccgcctg gcagtgtgca ctgcgtgacg atgacgtcta cctgacggta atgcctgcgt
     2041 ttcatatcga ttgccagtgt actgcggcga tggcggcgtt ttctgccggg gccacctttg
     2101 tgctggtcga gaaatacagc gcccgcgcct tctggggaca ggtgcagaag taccgcgcca
     2161 ccattaccga atgtattccg atgatgatcc gtacgttgat ggtgcagccg ccttcagcga
     2221 acgatcggca acaccgcctg cgggaagtga tgttttatct caacttgtcg gagcaggaaa
     2281 aagatgcgtt ttgtgaacgc ttcggcgttc gcttgctgac gtcttatggg atgacggaaa
     2341 ccattgtggg cattatcggc gatcgccctg gcgataaacg acgctggccg tcgattggtc
     2401 gggcggggtt ttgctacgaa gcggagatcc gcgacgatca caatcgcccg ctcccggcag
     2461 gtgagatcgg tgaaatctgt attaaaggcg taccagggaa aaccatcttc aaagagtatt
     2521 ttctcaaccc gaaagccact gcgaaagtgc tggaagccga cggctggcta cataccggcg
     2581 ataccggata ctgcgacgaa gagggctttt tttatttcgt cgatcgccgc tgcaatatga
     2641 tcaaacgcgg tggcgagaat gtctcctgcg tggagctgga aaatatcatc gccacccatc
     2701 caaaaattca ggatatcgtg gttgtgggta ttaaagattc gattcgcgat gaagccatca
     2761 aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga agaggaattt ttccgcttct
     2821 gcgaacaaaa tatggcgaaa tttaaagtgc cctcttatct ggagatcaga aaagatctgc
     2881 cacgtaattg ctcggggaaa ataattagaa agaatctgaa ataaatgaaa cagcagggaa
     2941 ctaccctgcc agctaataat cacgctataa aacaatatgc attttttgca gggatgttgt
     3001 cgtccctgaa aaagcaaaaa tggagaaaag gaatgagtga atcattacat ctgacccgca
     3061 atggatcaat tctggaaatt acccttgatc gtccaaaagc gaatgctatt gatgcaaaaa
     3121 ccagctttga aatgggcgaa gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg
     3181 ccattattac cggtgccgga gagaagttct tttccgcggg ctgggattta aaagcggcag
     3241 cagaaggcga agcaccggat gctgactttg gcccgggtgg atttgcggga ttaaccgaaa
     3301 ttttcaatct cgacaaaccg gttatcgcag ctgtgaacgg ctatgccttt ggcggcggtt
     3361 tcgaactggc gctggcggca gattttattg tttgtgccga taacgccagc ttcgccctgc
     3421 cggaagccaa gctgggcatc gttcctgaca gcggcggcgt gctgcgtcta ccgaagatcc
     3481 tgccgcctgc catcgtcaat gaaatggtga tgaccggcag acgaatgggc gcagaagagg
     3541 cgctgcgttg ggggatagtc aaccgcgtgg ttagccaggc ggaactgatg gataacgccc
     3601 gcgaactggc gcagcagctg gttaacagcg ccccgctggc gattgcggcg ctgaaagaga
     3661 tcttccgcac caccagcgaa atgccggtag aagaagcgta tcgctatatt cgcagcggcg
     3721 tgttgaaaca ctatccgtcg gttctgcatt cggaggatgc cattgaaggg ccgctggcgt
     3781 ttgccgagaa gcgcgatccg gtgtggaaag gacgttaaca accgtgagct attacgcctt
     3841 tgaggggtta attccggtgg ttcacccgac ggcgtttgtc catcccagtg ccgtcttgat
     3901 tggcgatgtg attgtgggag ccggtgtcta catcggccca ctcgcctcac tgcgtggtga
     3961 ctacgggcgg ttgatcgtgc aagcgggagc caatattcag gatggctgca ttatgcatgg
     4021 ctactgcgac actgacacca tcgttgggga aaacggccat atcgggcacg gagcgatcct
     4081 gcatggttgt gtgattggtc gcgatgcatt ggtcgggatg aacagcgtga ttatggatgg
     4141 cgcggtcatt ggcgaagaga gcattgttgc cgccatgagc tttgtcaaag caggctttca
     4201 cggcgagaaa cgccagttgt tgatgggtac gccagcccgc gctgtacgca gtgttagtga
     4261 cgacgagtta cactggaaac ggttgaatac caaagagtat caggatcttg tcgggcgctg
     4321 tcatgcatcg ttacatgaaa cacagccact gaggcaaatg gaggaaaatc gcccccgttt
     4381 gcaggggacg acggatgtga cgcctaaacg gtgatcttgc cggatgcgac ctggttgctg
     4441 gataagatgc tacaacatcg catctggcaa gcaggacgcc cggtatcaac gagataaaat
     4501 taacgacgca tactctttga caacatcatc tgccacttct gctcgcggtt aagttccgtg
     4561 agcggaacgg caggaatacg cggcgtcttt cttacgccga ctaatttatc ccggctgtta
     4621 ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca cttttaccag tcgctggcac
     4681 tggcatcccc gtccttccag cttattaggg atcatcttaa cttcgcagct tatttctgcg
     4741 acttccgaca gaatataagt cagggtatta accgccttgc tgtgttcaat atcgaaatga
     4801 atagagatct ctcttgctat cacccaccga ttttcagcca tcatccattc ggcgattaac
     4861 aagtagagtg gtttttcaac atatccttca cacatacgaa ctccagtttc acaaattaaa
     4921 agtctggcta tgactcgttt tgaagcaatt aaacaaggcc atattaaaat tgtggatata
     4981 tcgatagtat gcaacttcac cgtcgataag tgtgagctaa atcctgctta tgtcatcaaa
     5041 aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa aacggtcctc atcagagaac
     5101 cgtattactt attcgatcaa attagcggat gaaaaatatc cgccatgaca cgctattatt
     5161 tgatctgcgc gtgcatttcc tgcaccgaaa ttactttttc ggtcgcgtcg gcattcagcg
     5221 ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta tattgcagcg
     5281 cactgcgacg gatcacgcgg gagtcttcaa tcgcgcgacg gcctgaagtg gtgttgatga
     5341 tgtaggtata ttcgccattc ttgatgcggt cctgaatgtg cggacggcct tcatgcacct
     5401 tgttcaccag acgcggatta atgcccgctt cgcccagcac aatcgccgtg ccgtgggtcg
     5461 catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg cgttctttat
     5521 cgccttcgcg cacggaaagc aacgcacgac cgtgtttctt catggtggag ttgctgccca
     5581 gctgcgcttt ggcaaacgct tcagcgaagg tgcggcccac gcccatcact tccccggtgg
     5641 agcgcatttc tggccctaac agcgggtcaa cacccgggaa tttattgaac ggcagcacca
     5701 cttctttcac cgagtagtac ggcgggataa cttctttggt tacgccctgc tcagccagcg
     5761 atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg gctttggaga
     5821 cgaacggaac ggtacgcgcc gcacgcgggt taacttcaat caggtagact tcgttgtttt
     5881 tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc agtttctgca
     5941 cctgctggcg catcacatcc tgaatttcct gacttaaggt gtaggctggc agagaacatg
     6001 cggagtcacc ggagtgcacg cccgcctgct cgatgtgctc catgatgccg ccaatcagca
     6061 ccatttcgcc gtcgcagatg gcatccacgt caacttctac cgcgtcatca aggaagtgat
     6121 ccagcaacac tggcgcatcg ttagacacgc tgaccgccgt ctggaagtag cgacgcaggt
     6181 cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac ggacgtacca
     6241 ccagcgggta gccaatctct ttcgccttct ctaccgccat ttcaatagcg gtaacggtgg
     6301 cgttcgccgg ttgtttcagt ttcagacgct caaccgcatg ctggaagcgt tcacggtctt
     6361 ctgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagca gcttccagcg
     6421 cgcgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct ttcggcttct
     6481 caatacgcac gatttctagc acatcttcca gtgttaccgg ctcgaagtag aggcgatcgg
     6541 aagtgtcgta gtcggtggag acggtttccg ggttacagtt aaccataatg gtttcgtaac
     6601 cgtcttcgcg cagtgccagc gaggcgtgta cacagcagta gtcgaattcg ataccctgac
     6661 cgatacggtt cgggccgccg ccgagaacca tgattttttc acggtcggta gacggattcg
     6721 cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggcg aactctgccg
     6781 cacaggtatc cacgcgctta taaaccgggt gcaggtcata ctggtcgcgc agcttgcgga
     6841 tttccgcttc gcgcacgccc gccagttttg ccagacgcgc atcggcaaag cctttgcgtt
     6901 tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgct actttctctt
     6961 ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatattg gtcaggttga
     7021 acacgccgtc cacagacagg cccgcacgga aggcatcggc gatgtaccag atacgctctg
     7081 cgccagcatc tttcagttcg cgacggattt tggttaacgc ttccgggtca tcgaggctca
     7141 ctttcgggtc gaatccagtc gcaccgactt ccaggccgcg cagcgctttt tgcagggatt
     7201 cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga gtggtcagac
     7261 ggtcgttagc accagcgaat ttttcgaagt tgaagcgagg aattttagta accacatagt
     7321 cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc atcagttcgt
     7381 cgagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa ccggtcgctt
     7441 tcgacgccag cgccgaagaa cgggacacgc gcgggttcat ttcgataaca atcaggcgac
     7501 cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg ccgatttcac
     7561 gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc agcgtttggg
     7621 ctggcgcgac agtgatggag tcaccggtat ggatgcccat cgcatcaaag ttttcgatag
     7681 agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg tactctttcc
     7741 agccaatcag cgactcatca atcagcaact ctttggtcgg agagagatcc agaccgcggg
     7801 cgcaaatttc ttcaaactct tcgcggttat aagcgatacc gccgccgcta ccgcccatgg
     7861 taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaaccgcc agcgcttctt
     7921 ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc atcgctacgt
     7981 cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc ggtcgcacca atcatagtca
     8041 cgccgaactc ttccaacacg ccctgacgct ccagctccag cgcgcagttc agcgccgtct
     8101 gaccgcccat cgttggcagc accgcgtccg ggcgctcttt ttcaataatc ttgcgtacca
     8161 cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg gtcatgatgg
     8221 tcgccgggtt ggagttcacc agaatgaccc ggtaaccctc ttcgcgcagg gctttacatg
     8281 cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg cccgcaccca
     8341 gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc ctgattactt
     8401 agcggtttta cggtactgct caattaactc gataaagtgg tcgaacaacg gcgcggcgtc
     8461 gtgtggacca gggctggctt cagggtgccc ctggaagctg aacgccggtt tatcggtgcg
     8521 atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca ggtttgctgg
     8581 taatgttgct tcgtccaccg caaaaccgtg gttctgggcg gtgatcatca ccacgttttt
     8641 ctcaacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt tgacagtctt
     8701 cgcaccgctc gccagcgcca gcagctgatg accgaggcag atgccgaaga ccggaatatc
     8761 ggtttcgagg aatttctgga tggcggtaat ggcgtaatcg cacggggccg ggtcgccagg
     8821 accgttggag aggaagatac cgtctggatt cattttcagc acatcttccg cagaagtttg
     8881 cgccggaacg atggtcaggc gacagcctct gtccaccaac atgcgcagga tgttgcgctt
     8941 ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt tcgcttctgg
     9001 caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg cggtggtcac
     9061 ttcttttgcc agatccatgc cattcagacc tgggaacgcg cgggcttttt ctaacgccaa
     9121 cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt tctcgcgcag
     9181 taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat ggcgcttcag
     9241 gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca ggtcgcgaat
     9301 caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag tgccgacatt
     9361 gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat cagtgaggat
     9421 ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg aacctgttgc
     9481 ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg ctgacttaat
     9541 caaaacaccc tccagagaat attcactcac tttatttgca tattaattca caatgatgac
     9601 gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca ttctggagat
     9661 atgggcgcta aaagtcaact taatggcggg attttttata ttattttgtg gctcatgcat
     9721 gttttacagg cattaagcgg caaaaagatc atataacctg ttctggaaaa cgcttgcgca
     9781 gcaagaagat tgcatgaaat aaaagcgagt agggaaaagt ggaccaaatg gtcaaaattt
     9841 acattaaaac aacaaaaacc gaagacaaaa catgtaattt tt
//
LOCUS       DAZFJF010000154         9827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFJF010000154 DAZFJF010000000
VERSION     DAZFJF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.155"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(10..888)
                     /gene="glxR"
                     /locus_tag="ACHKAU_004290"
     CDS             complement(10..888)
                     /gene="glxR"
                     /locus_tag="ACHKAU_004290"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5533242.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(988..1764)
                     /gene="hyi"
                     /locus_tag="ACHKAU_004291"
     CDS             complement(988..1764)
                     /gene="hyi"
                     /locus_tag="ACHKAU_004291"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO5533243.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(1777..3558)
                     /gene="gcl"
                     /locus_tag="ACHKAU_004292"
     CDS             complement(1777..3558)
                     /gene="gcl"
                     /locus_tag="ACHKAU_004292"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO5533244.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3648..4463)
                     /gene="allR"
                     /locus_tag="ACHKAU_004293"
     CDS             complement(3648..4463)
                     /gene="allR"
                     /locus_tag="ACHKAU_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO5533245.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4541..5023)
                     /gene="allA"
                     /locus_tag="ACHKAU_004294"
     CDS             complement(4541..5023)
                     /gene="allA"
                     /locus_tag="ACHKAU_004294"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO5533246.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5253..6179
                     /gene="allS"
                     /locus_tag="ACHKAU_004295"
     CDS             5253..6179
                     /gene="allS"
                     /locus_tag="ACHKAU_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO5533247.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6248..7342
                     /gene="mnmH"
                     /locus_tag="ACHKAU_004296"
     CDS             6248..7342
                     /gene="mnmH"
                     /locus_tag="ACHKAU_004296"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO5533248.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            7458..7823
                     /gene="ylbG"
                     /locus_tag="ACHKAU_004297"
                     /pseudo
     CDS             7458..7823
                     /gene="ylbG"
                     /locus_tag="ACHKAU_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(8590..8836)
                     /locus_tag="ACHKAU_004298"
                     /pseudo
     CDS             complement(8590..8836)
                     /locus_tag="ACHKAU_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8817..9827)
                     /locus_tag="ACHKAU_004299"
                     /pseudo
     CDS             complement(8817..9827)
                     /locus_tag="ACHKAU_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         2504 a         2594 c         2253 g         2476 t
ORIGIN      
        1 ttgcgggtat caggccagtt tatggttagc cattaattcc agcgcctgca ctaacgcaga
       61 gtgatccaac tggctgccac cgtttgccgc gcaggtatta aataactcct ggcaggtcgc
      121 agtgtttggc aggttcagcg ctagcgcttt cgcactttgc agtgccaggt tgagatcttt
      181 ctggtgcagc gcaattttga aacctggatt aaaagtgcgt ttaatcatgc gctcaccgtg
      241 aacttccaga atacgtgagg aagcaaagcc gcccatcagc gcctggcgca cacgtaccgg
      301 gtccgcaccg gcttttgaag caaatagcag ggcttcagaa accgcttcaa tattgagcgc
      361 cacaataatc tgatttgcca ctttgcaggt ttgaccatcg ccgttaccgc ccacgagggt
      421 gatattttta ccgagcagtt caaacagcgg tttaacacgt tcaaataccg cttcatcacc
      481 gccaaccata atcgacaacg tcccttcacg cgcaccgatt tcaccgccgg agactggcgc
      541 atcgagataa tcgccgccca gttcattcac ctgacgagcg aaacgcttag tttcaatcgg
      601 ggaaatggag ctcatatcaa caatggtttt gcccttcagc gaggctttgg tacaaccatt
      661 ttcaccgaac agaacttctt caacctgtgg tgtatctggc accataataa aaatgatgtc
      721 cgatgcttcc gttacctggc gcgcagtttc aacactgacg gcacccagtg acagtaattc
      781 atcagcaacc ggaccaatgg ttgtgacatg cagttgatga ccggcacgcg ccagattaat
      841 ggccatcggt gtacccataa tgcctaagcc aataaatccc agtttcataa ttaacctctt
      901 ttaaatttcg cttttcctga attcagacaa cacgatcccc tgccaactta gcggcctaaa
      961 aaagcattgc ctgaacagcg ttacgtttta acggtacgga tccatccagc gtaaaccggc
     1021 ttccgtggtg gtttggggtg tatattcaca cccaacccaa ccgttgtagt cagaattttc
     1081 gattacctta aagagataat cataattaat ttctccggtc cccggttcgc cgcgatgcgg
     1141 attatcagca atttgcaggt gaccaatttt atcggcccac tgagtcatgg tgttggttaa
     1201 ttcaccttcc atccgctgca tgtgataaat gtcatactga attttcaaat tgcagcaacc
     1261 aacatcatca atcaatttca gcgcctgccg agttccggtg agatggaaac caggaatatc
     1321 aaaatggtta ataggttcaa tcagtaataa aatatcttct ttcatcagca tattcgcggc
     1381 ataacgcagg ttttctacaa gcgttgcgtg aatctgttca ctgctgaaac cagccggcgt
     1441 tttaccgacc agacagttaa tttttttatt acccagcgca cgggcataac gaatcgctgc
     1501 tgctacgcca tcccgaaact cttcttcacg gccaggaata caggcaatac cgcgctcccc
     1561 cgccgcccag tcacctgccg gtaaattgtg cagcgtatgt tcgagtttat tactcgccag
     1621 cacctgtttt aattcttcaa tgtcgtagtc ataaggaaac ataaattcaa cgccgcgaaa
     1681 accacactgc gcagctttct caaaacgggc gagaaaatca tattcaccaa ataacatcga
     1741 taaattagca gagaaacgta acattattta tctcccttat tcatagtgca tgaagcaggt
     1801 ttcagtcggt gcgtccgctg cgttatcggc gatatcttca aattccatga cgttatccag
     1861 ttcgctgccc atcgaaatat tggtcacacg ctcaagaata acttccacga ctaccggtac
     1921 ccgatattgc gccattaagg ctttcgcctg ttcaaaggct ggcgcaatat cttccggttt
     1981 gaagacccga atagctttac aacctaaacc ttccgctact tttacgtggt cgacgccgta
     2041 accgttcact tcactggagt taatattttc gaacgcgagc tgtacgcagt aatccatgtc
     2101 aaaggcgcgt tgtgactgac gaatcagccc cagataagcg ttattgacca gtacatggat
     2161 gtacggaatg ttgaactgtg cgccaacggc taattcttca atcaggaact ggaagtcgaa
     2221 gtcaccggaa atcgccacca cattccgttc tggatcagca gcacacacgc ccagcgccgc
     2281 cggaatggtc cagcccagcg ggcctgcctg accacagttg atccagtgac ggtctttaaa
     2341 gacatgcagc atttgtgccg cagcgatttg tgacaggcca atggtggtga cataacaaac
     2401 atcgcgacca aaggctttgt tcatctcttc atacacgcgc tgcggtttca ccggcacgtt
     2461 gtcgaagtgg gttttgcgca gcaaagtgcg tttgcgctgc tggcagtcgg cgacccattc
     2521 tttacgacac ggcagacgac ccgctttttg catctcctgc gccacttcaa ccagcagtgt
     2581 cagcgccgct ttagcatcag agacaatgcc gagatccgga cacagcacgc gaccaatttg
     2641 cgtcggctca atatcgatat gaacgatttt gcgcccttcg gtgtatttct ctaccgaacc
     2701 ggtatgacgg ttagcaaaac ggttaccgat accaaacacc atgtcggacg ccagcagcgt
     2761 tgcgttaccg taacgatgcg cggtttgcag acccaccatc ccggccatca gttcatgatc
     2821 gtccgggata cagccccagc ccatcagcgt tgggatcacc ggaacgctgg tcagttcagc
     2881 aaactgttgt aacagtgcag ctgcgtcagc attaattacc ccgcccccgg caacaatcac
     2941 cggacgttcg gcctggatta acatttctac agctttttcg atctgcatac ggctggcagc
     3001 aggtttgtag accggcagcg gttcgtacat atcaggatca aactcgattt ccgcaacctg
     3061 aacgtcgaac ggtaaatcca ccagtaccgg acccggacga ccagaacgca tcaggtgaaa
     3121 tgcctgttgc aacacacgag gcaccagcgc cgcttcacgt actgtaaccg ccattttact
     3181 taccggcttg gcgatagctt cgatatcaac agcctggaaa tcttctttat gcagacgggc
     3241 gcgcggagcc tggccagtaa tacacagaat aggaatggaa tcagcagaag cagaatagag
     3301 cgcggtgatc atgtccgtgc ccgcaggacc ggaagtcccc agacatacgc cgatattccc
     3361 tgccgttgcg cgggtataac cttccgccat gtgcgaagca ccttccacat gacgcgccag
     3421 aatgtgacga ataccgccgt gcttacgcat cgctgagtag aacggattga ttgcagctcc
     3481 cggaacgccg aaggcggtgg tgataccttc tttctccagc acatacattg ccgcgtcaac
     3541 ggctctcatt tttgccattt tattccaacc tctattaatt ggaaaaattt tccaactttc
     3601 aaattttgtc aaaaaaaccc gctccgcaga gcgggatgcg acagacacta tggatgtgct
     3661 ttcagtccca acgccgtgct gatatcgcgg gcggtgtctc tgaccagctc accctgactg
     3721 acaaaacgat cttctgtcag tcttgatgaa ggcccggaga tggagatagc ggcaacaacg
     3781 ctaccgacat catcgtaaat tgctgaagct atgcaattca gacctacaac atgctcttct
     3841 ttatctacgg tatagcccag ttcacgcgct tgttccaggt ccttcagcaa ggtgggcata
     3901 tccacaagcg tagttggcgt aaactgctgc aaaccggttt gcagaatgat gctcatcaac
     3961 tcctcttccg ccagcggata aagcaacgcc ttgcccgcac cggaagcatg cagcggcaga
     4021 cgactgccca gtggcgcaca catcctgacc atcgatttac actctaactg accaattaat
     4081 accgcttcat tgccgttacg gatcgcgaca ttgaccgttt cgccggaaag taacatcagg
     4141 cggcgcataa acggcccggc gacggagagg acatcgcggt tatggatgta cgccgcaccg
     4201 acgttaaaga cacctaatcc tatatgccac cagcctaact gactgtcctg atagacaaaa
     4261 tccgctgcct gtaaaacctt cagcaagcga aaggtcgtag agagcggcaa atccagattg
     4321 agagaaatat cgctaaccga cgaacttccc ccactttttt ccagatattg cagaatcgca
     4381 attccccgct ctaacgcctg tgcgcccttc tgtgccacag gctccgcctg tcctggcctg
     4441 ccgcgccgtc taacttccgt catacctacg ttaactccga cctacaagtg agcataaaca
     4501 atgaacaatt agtttatctt atgcaagtcg gttgcaggcg tcacgcaaaa cagagttcct
     4561 gttcaggaat actttcaaca tcacagttgt cactgccgcc gcgatcgatg gtcagaaaat
     4621 cggtgacgcg ctgccaggcg aaaagtgggt gatgccagac gttacggtgg taattcactc
     4681 cctgttcgcc gttggtaata aacgcccgca gggttgacag gtctggtttg tcgtcaccta
     4741 acgccacgac caccacaaac acctcacctt tcatcgggat aaaggcctga gtacccagcg
     4801 gatgacgttc gagttcgtga atggtcagcg gcagattcgc cggttgcgcg cggttaatgc
     4861 tgataagcgt acggtcttgc tcaagaatct caaccagcgc caaatcgtgg taacgctcca
     4921 ccaggccatt gttaatatgg aaaaaatccc gttgctgcgt ttcgattacg tcgccataag
     4981 cactaaaggc ttcctgactt aacggtaata cctgaagttt catctttttg tccttgtaac
     5041 ataattccgt tcaaccaccg ccgaccggtc agcttggttt caataacagg caatctaaaa
     5101 ccttaagaga acaacatcaa tacaaaagtt ggaatatttt tccaaaacta gatttagtcc
     5161 acaaattgaa cagggttatc atgtaaaaat actgataaat cagaggataa gaaaatcacg
     5221 caacttgcgg atttaaaagg ggatacctga agatgttcga tccagaaacc ttgcggactt
     5281 tcattgcggt tgctgaaaca ggaagttttt caaaagcggc agaacgatta tgtaaaacca
     5341 cggcgacgat cagttatcgc attaaacttc tggaagagaa taccggagta gcgctgtttt
     5401 tccgcacgac tcgcagcgtg acgttgacag cggctggcga gcatctactt tcccaggcca
     5461 gagactggct gagctggctg gaaagtatgc caagcgagct gcaacaggtg aatgatggcg
     5521 tggaacgcca ggtgaatatt gtcatcaaca acctgctcta caacccccag gccgtcgccc
     5581 agttgctggc gtggctgaat gaacgttacc cctttaccca gtttcacatc tcccgacaaa
     5641 tctatatggg cgtctgggat tcgctattat acgaaggttt ttcactggct atcggcgtca
     5701 cgggaaccga ggcgctggca aataccttta gtcttgatcc cttaggatcg gtgcaatggc
     5761 gctttgtcat ggcggcggat catccgctgg cgaacgttga agagccgcta acagaagcgc
     5821 agttgcggcg ctttccggcg gtcaatattg aagacagcgc ccgcacctta accaaacgcg
     5881 tcgcctggcg attgcctggg caaaaagaga ttattgttcc tgatatggaa acgaaaatcg
     5941 ccgcccatct tgcgggcgtt ggcattggtt ttttgccaaa atcgctttgc cagtcaatga
     6001 tcgataatca acaactagtt agccgggtaa tcccaacgat gcgccctcct tcgccattga
     6061 gtctggcatg gcgcaaattt ggcagcggca aagcggtaga agatattgtg accttgttta
     6121 cccagcgcag gccggaaatc agcggatttt tagaaatttt cggcaaccca cgcagttaaa
     6181 aatcaagttt ccttgcgcgt tggcgcgtaa aatagcgcgc aagctgattt gaggttcact
     6241 tttaactatg caagagagac acacggaaca ggactatcgt gccctgctga ttgctgatac
     6301 gcccattatt gatgttcgcg cccctatcga gtttgagcaa ggcgcaatgc ccgccgctat
     6361 caatctgccg ttaatgaata acgatgaacg cgccgccgtt ggcacctgct ataaacagca
     6421 aggctcagac gcagcgctgg cgctggggca taaactggtg gcgggtgaaa ttcgtcagca
     6481 gcgcatggac gcctggcggg cagcgtgcct gcaaaatccg caaggtattc tctgctgcgc
     6541 ccgtggcggt cagcgctcac atattgtgca aagctggttg tatgcagcgg ggattgatta
     6601 tccgctggtg gaaggcggtt ataaggcact gcgccagacc gcgattcagg cgactattga
     6661 actggcacaa aaaccgatag tgctgattgg cggttgtact ggcagcggta aaacgctgtt
     6721 agtgcagcaa cagccgaacg gtgttgatct ggaagggctg gcgcgccatc gcggttcggc
     6781 gtttggtcgc acgttacaac cacaacttag ccaggcgagt tttgaaaacc tgctggctgc
     6841 cgaaatgctg aaaaccgacg cccgtcagga tttgcgcctg tgggtgctgg aagacgaaag
     6901 ccggatgatc ggttcgaatc acctgccgga atgcctgcgc gagcgaatga ctcaggcggc
     6961 gattgcggtg gtagaagatc cgtttgagat ccgtcttgag cgcctgaacg aagagtattt
     7021 cttgcgtatg catcatgatt ttacccacgc ttatggcgac gaacagggct ggcaggaata
     7081 ttgcgaatac ctgcatcacg gactttcggc gattaagcgt cggctggggt tacagcgcta
     7141 taacgaactc gctgcccagc tggatacggc gctgacaacg caactcacca ccggcagcac
     7201 cgacggtcat ctggcctggc tggtgccgtt acttaaagaa tattacgacc caatgtatcg
     7261 ctatcagctt gagaaaaaag cggaaaaagt tgtctttcgc ggtgagtggg cggaagtggc
     7321 ggaatgggtt aaggcgcggt aatgagggac gtatttgtat tagtcgcgac ttgatctgac
     7381 acatggcctt gaaaggttga gagttaccgg ttttgatatg agtgtcgaat ccttatccaa
     7441 aacaagaggt aactctcatg cttcatactg ccaatcccgt catcaaacac aaagccggtt
     7501 tgctcaatct ggctgaagaa ctcagcaacg tatcaaaagc ctgtaaaatc atgggcgttt
     7561 cgcgtgatac gttttatcgt taccgcgaac tggtcgctga aggcggtgtg gatgcgcaga
     7621 ttaatcgtag tcgccgcgct cctaacctta agaaccgtac cgatgaggca actgaacagg
     7681 ctgttgttga ttacgccgtc gctttcccga cacatggtca gcaccgggcc agcaacgagc
     7741 tgcgtaaaca gggggttttt atctccgaca gtggtgtccg ttctgtctgg ttactccata
     7801 accttgagaa tctcaaaaga cgatactgaa caaattttat caggtgacgt tccgtaaaaa
     7861 gttgtatgga gttctcgatg cagtcggcgc gactatcagt acccccgctg aaccctggac
     7921 tatcgggccg ccgtactggg gcatgtcacc ctggcgtgcc gctggttttc cgctaatggt
     7981 ttatttcctc tttctttccc ggcaagaatt tattttaatc cggaaggatt atttattttc
     8041 agatagggac tcacattaaa cacgtaacat tttaattaat gcatggctga atatgtatta
     8101 cacaatgtca gatatcgtaa aagaccagtg caattctatg taaactctct gactgttcat
     8161 tttatttgtt gtttcagggt cggaactcac cctcaataaa caactgaata cgagtagata
     8221 ttcatcgtct gagctatatg gctttacaca atagccgaca tgtcttttct cagtcaggaa
     8281 ttttactttt cctcactacg aagttgacga cttcgatatg ggatagactc ttaattcaag
     8341 caattactct gcacaagcat caggcaatcc agctccaata tttgttataa aataatgcgt
     8401 aacattaatt tcaccaacaa aaaaaaggtt tacaccatcc ccttgccctg gtatcttacc
     8461 atatttacac cacacttttt ttgtatctgg atcattcttt gatacgattt tcgccacttg
     8521 ttccctggcc ctcactgtca atgttttctt aaacctaccc ccatcatcat ttgttaggga
     8581 aaaataagaa aaaaaataat catcttctac atttgtgtat attgatatat ccaccgctac
     8641 gtgcggaaaa aaaatctggg agccatgtaa aaactccatc attttctggt attgccatat
     8701 agtttatgta atgtttactt acaagagatt catctttgta taaataaaga taagtaatta
     8761 cgcataaaac aacaatgatt ataatagcaa aaataaatat tatcatcttt gatagattac
     8821 ttgagatagc cagcatcttg taaagccttt atcgtttttt tatgctctgg attaatataa
     8881 tcactacatc tatctgagca atctgttgtt gatggacatg tcaacccatg gtcatttaca
     8941 gccaagtctt ttttattatc ttcaatcatt tcagaatggg atagctttac ttttctgccg
     9001 tacataccga acagatttaa cccgtaatct ctaatctctt tttcataacc cagccctttc
     9061 gccgatcggc taacaccagc gtcatttaat ttagacactc gacaaaatgc catacaatgg
     9121 aaaaattgat cagtcccacc taaatttaat ctcttcatat cttcataagt atcagataat
     9181 atatcccatg ctctttgatg gtttagttga tcttttcttc ttattagcgc tacatctgcg
     9241 ggacttaacc ctaatggatc aataccattc accggattca gcgggtacgc atacagactc
     9301 catccccccc tccagcccta tcgggtcctg agtgatatac cgcccctgca acggatcgta
     9361 gtaccggttc cggttgtagt acagccccga ctccttatca tactgctgcc ccggcagccg
     9421 gtacggctgg tgcaggtgat gcgggttctc ctcattaagc tggttgcccc attcatcata
     9481 ttccgcgctc cacgccgtat tgccgtcttc actgataagc gccagcggca ggccccggtg
     9541 gtcacagtga taaagatgta tttttcgctc aggcaaataa accggctcta tctgtgccgc
     9601 cagccgctcc accgtcagcc cgcactgcgc aagccacgcc cggctttcgt cactcacgcg
     9661 gtctgcccgg atttcttcct ccagtctgtc cagcagccgc accagttcag ccgggaacac
     9721 cacaccgtga ccgtcttcgc tcccttcctg ctgtaacttc tccgccaggc tgcggcgctg
     9781 cgctttctcc cgctcgccgt tctctgtctc gacccggatg agcggcg
//
LOCUS       DAZFJF010000155         9723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFJF010000155 DAZFJF010000000
VERSION     DAZFJF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.156"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1176
                     /locus_tag="ACHKAU_004300"
     CDS             <1..1176
                     /locus_tag="ACHKAU_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5533249.1"
                     /translation="NAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHK
                     PINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALI
                     PFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRT
                     RLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIF
                     QELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWG
                     HRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVEL
                     LTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1357..2847
                     /gene="dgcJ"
                     /locus_tag="ACHKAU_004301"
     CDS             1357..2847
                     /gene="dgcJ"
                     /locus_tag="ACHKAU_004301"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO5533250.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2890..3393
                     /gene="yeaK"
                     /locus_tag="ACHKAU_004302"
     CDS             2890..3393
                     /gene="yeaK"
                     /locus_tag="ACHKAU_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO5533251.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3394..3498)
                     /gene="yoaI"
                     /locus_tag="ACHKAU_004303"
     CDS             complement(3394..3498)
                     /gene="yoaI"
                     /locus_tag="ACHKAU_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO5533252.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3668..4114
                     /gene="yeaL"
                     /locus_tag="ACHKAU_004304"
     CDS             3668..4114
                     /gene="yeaL"
                     /locus_tag="ACHKAU_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO5533253.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4071..4892)
                     /gene="nimR"
                     /locus_tag="ACHKAU_004305"
     CDS             complement(4071..4892)
                     /gene="nimR"
                     /locus_tag="ACHKAU_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO5533254.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4989..6170
                     /gene="nimT"
                     /locus_tag="ACHKAU_004306"
     CDS             4989..6170
                     /gene="nimT"
                     /locus_tag="ACHKAU_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO5533255.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6225..6572
                     /gene="yeaO"
                     /locus_tag="ACHKAU_004307"
     CDS             6225..6572
                     /gene="yeaO"
                     /locus_tag="ACHKAU_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO5533256.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6594..6848)
                     /gene="yoaF"
                     /locus_tag="ACHKAU_004308"
     CDS             complement(6594..6848)
                     /gene="yoaF"
                     /locus_tag="ACHKAU_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO5533257.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7031..8056
                     /gene="dgcP"
                     /locus_tag="ACHKAU_004309"
     CDS             7031..8056
                     /gene="dgcP"
                     /locus_tag="ACHKAU_004309"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO5533258.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8089..8187
                     /gene="yoaK"
                     /locus_tag="ACHKAU_004310"
     CDS             8089..8187
                     /gene="yoaK"
                     /locus_tag="ACHKAU_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO5533259.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8190..8264
                     /gene="yoaJ"
                     /locus_tag="ACHKAU_004311"
     CDS             8190..8264
                     /gene="yoaJ"
                     /locus_tag="ACHKAU_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO5533260.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8323..8571)
                     /gene="yeaQ"
                     /locus_tag="ACHKAU_004312"
     CDS             complement(8323..8571)
                     /gene="yeaQ"
                     /locus_tag="ACHKAU_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5533261.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8799..>9723)
                     /locus_tag="ACHKAU_004313"
     CDS             complement(8799..>9723)
                     /locus_tag="ACHKAU_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5533262.1"
                     /translation="KRAAFPRFKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRN
                     SRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTV
                     FGPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTT
                     TVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEY
                     KQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNIL
                     AAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2377 a         2367 c         2284 g         2695 t
ORIGIN      
        1 aacgccgtca tttttctttt catgttaatg aaatatttca ctaacaaaca aattttacca
       61 acactcattt taagccttgc atttttaagt ggccttatct atttagttga aaccattgta
      121 attatccata aaccaattaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct
      181 attttctata ttttccgcca actcagtttt atttgtttaa cctcgctggc actcttttgt
      241 tatggaaaag acaacatcct tgacaacaat aagaaaaaaa cgggaatcct gttgctggcg
      301 ctgattcctt ttttagtttt tccccttctg gcacacaatc tgagcagtta taacgctgac
      361 tattctttgt atgtcgtcga ctactgtccg gacaaccata ctgcgacctg gggaatcaac
      421 tatacaaaaa tattggtttg tctgtgggca tttttactgt tctttattat catgcgtaca
      481 cgattagcca gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc
      541 aacttacttc tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg
      601 attgaagttt ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcaggaa
      661 ctgcaactct ccagcaaact ggcagttcat gatgtgctga ccaatattta taatcggcgc
      721 tactttttca acagcgtaga gtcattattg tcgcgacctg ttgttaagga cttctgtgtc
      781 atgctggttg atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt
      841 gataaagtgc tggtttcaat tgtcgatatt atccagcaaa gcatccgccc tgatgatatt
      901 ttagcgcgac tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag
      961 gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac
     1021 agatatgatg ttcctgaaca aatgaccatc agtattggca cggttttttc aacgggtgac
     1081 acgcgtaata tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc
     1141 gaggggggca acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc
     1201 tttttgcgtg gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc
     1261 ggcgcagaga tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt
     1321 tttctcgttt cactaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc
     1381 cggcatttta tcgccgcaag tgtcattgtg ctgacatctt ccttccttat ttttgaactt
     1441 gtcgccagcg acagagcaat gagtgcctat ctgcgctata tcgtgcagaa agcagactcc
     1501 tcctttcttt atgataagta tcagaatcag agtattgccg cgcatgtgat gcgcgctctc
     1561 gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag
     1621 tctgccaata atacccatgg cttaaacctg actgcccata aatacccggg cttacgcggc
     1681 acactacaaa ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc
     1741 gcttttgatc aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg
     1801 atggccgaag agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat
     1861 gagccggtta atgttgaata ctttgcgatg aataactggt ccttcctgca gtcaggaagt
     1921 attggcatcg atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc
     1981 atttaccagg atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc
     2041 gcagggcagc taaaagggat tgtgctgctg gatattaaca aaaacaattt gcggaatatc
     2101 ttttacactc atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgatacc
     2161 gattcggggc gcgacattat catcaaccag agcgaagata atctgttcca gtacgtcagt
     2221 tacgtccatg acttaccggg cggcattcgt gtctcgttat ccattgatat tctttacttt
     2281 atcacgtctt cgtggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat
     2341 atggtccgga tgcacttccg tttataccaa aatgtgtcgc gagaaaatat tagtgatgcg
     2401 atgactggac tgtataatcg caaaatttta acccctgaac tggagcagcg gttgcagaaa
     2461 ctggtgcaat ccggttcttc ggtgatgttt attgcaattg acatggacaa gttaaagcaa
     2521 ataaatgaca ccctcggtca tcaggagggg gatttagcga ttacgttatt agctcaggcg
     2581 attaaacaat cgattcgtaa aagtgattat gccatccgac tcggtggcga tgaattctgc
     2641 atcattcttg tcgattcgac gccgcaaatt gcagcacaac tgcctgaacg tatcgaaaaa
     2701 cgtctgcaac atatcgcgcc gcagaaagaa atcggcttct cttccggtat ttacgcgatg
     2761 aaagaaaacg atacgttaca tgatgcgtat aaagcttccg atgagcgttt atatgtcaat
     2821 aagcagaaca aaaacagccg ttcatgataa ccttctgtgg ttgtttgctt gtaatctcag
     2881 gagcgtgaaa tgactgaaat ggctaaagga agcgtgacgc atcagcggtt aatcgcttta
     2941 ttatcacaag aaggtgctaa cttccgcgtc gttacccatg aagcggtagg taaatgtgaa
     3001 gcggtgtcag aaattcgtgg caccgctcta ggccagggtg ctaaggcact ggtatgtaaa
     3061 gtcaaaggca atggcgtaaa tcaacatgtt ctggcgatcc tcgctgccga tcaacaggcc
     3121 gatctgagcc aactcgccag ccatataggc ggattacgcg cttcgttggc cagcccggcg
     3181 gaagtcgatg aactcacagg ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca
     3241 aaactcaaac tggttgccga ccctctactc ttcgaacgat ttgatgaaat cgccttcaat
     3301 gcagggatgc tggataaatc cgttattttg aaaaccacgg attatttgcg cattgcgcaa
     3361 ccagaactgc tcaatttccg ccgcactgcg taactagcct gtccgttcaa taagcagaac
     3421 ggacaaaacc agcacaacag caatggcaaa aaacgatgac gtgataatca gtgtctcgac
     3481 gaacatttga tcgttcatag catgcccccg ggagatagct gttttcacgt tattgttagc
     3541 gtgcacaaat ggcagtttga tgacagttcg cctgattttt tatgcaaaaa acgtaaagat
     3601 tttctacttc cttcctgcag caagcgtaaa gtaagcaggc ttattatttt ttggcaagga
     3661 aaccacgatg tttgatgtca ctttgctgat cctgctcggg ttagctgcgc tgggctttat
     3721 cagtcataac accactgtcg ccgtttcaat tctggtgttg atcattgtcc gcgtcacacc
     3781 gttaagcacc tttttcccgt ggattgaaaa acaggggcta agtatcggga taatcattct
     3841 gacgattggc gttatggccc ccatcgccag cgggacgcta ccgccctcaa ccttgattca
     3901 ctcgtttctg aactggaaat cgctggtggc aattgcagta ggcgttattg tctcctggct
     3961 gggtgggcgc ggcgtgacct taatgggcag ccagccgcaa ctggtcgccg ggctgctggt
     4021 aggcactgtt ttaggcgtgg cgctgtttcg cggcgtaccg gtcggcccgc ttattgccgc
     4081 gggtctggtt tcgctgattg tggggaaaca gtagttaatc ccgcaatata tcggcctggc
     4141 gtttgcccca ggcctttctt aaacatagta ataaaggccg ttgttgaatc ataccccagc
     4201 gtatgagcaa ctttctgtac agtatcgcct ttcactaatc cctgaagtgc cataatcagc
     4261 tgtaactgct gacgccactg gcgaaaactc aaccccgtct cttttactat cagtcgcgca
     4321 agattgcgtt cactcatcgc aaaaaagcca gcccattgcc ctaatgaccc ccactcgacg
     4381 ggccctttcg ccatcatctc caccatcgtg cggattttag gatgagaaga aacgggcagg
     4441 tgcagctgtt gttgtggctg ctgagggagt tcatcaaaca ggacctgaat taatctgcgg
     4501 gtcatcggct cagcacgctg agtcgtcgtc ctgtttgcca gggttaaaat caattcccgg
     4561 cataagggcg atattttcaa tgtgcaacat gttgtcggca ttgtcactgc gctgggttcg
     4621 ataaacagaa aacagagttc agcgttagct gtcacttgat tgctatgttc caccccgccc
     4681 ggtatccaaa ccgcatattg cggcggcacc atccacaaag cattttccac cgtacaggta
     4741 attgcaccat gtaatgctag tatcagctgt cctttgcggt gctgatgtac aggactggtt
     4801 aactcatccg ttccggcgtg aatacaaaat gcgaccgcgg cttcatggtg tcggtcgggt
     4861 tcatagccat tgagattcaa cctgtgcatc attttgtccg aatttagcga taatttgtca
     4921 ttttagcttg attcaacata acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg
     4981 agaagcatat gacctgttca acttcattaa gcggcaaaaa caggattgtc cttatcgctg
     5041 gcattctgat gattgccact acattacgcg tcaccttcac cggcgcagca ccgttactcg
     5101 atacgattcg ttccgcttac tcgctgacga cagcgcaaac cggcttatta accaccctgc
     5161 cattattggc ctttgcgcta atctcacctt tggctgcccc ggtggcgcga cgttttggta
     5221 tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca attcgttctc
     5281 tcccttcgcc ttacttatta tttggcggta cagcggtcat tggcggtggg attgcattag
     5341 gcaatgtctt actgccagga ttaattaaac gcgatttccc tcattccgtc gccagactta
     5401 ccggcgcata ttccctgaca atgggagctg cagcggcact gggatcggct atggtcgtgc
     5461 cgctggcttt gaacggtttt ggctggcaag gcgcgttgtt catgctgatg tgttttcctc
     5521 tgctggctct ttttttatgg ctgccacagt ggcgaagcca acaacatgca aatttgagta
     5581 cctcgcgcgc cttacatact cggggtatct ggcgttcgcc gcttgcctgg caggtcacat
     5641 tgtttcttgg gatcaactca ctggtctatt acgtgattat tggctggctt ccggcgatcc
     5701 tcatcagtcg cggctatagc gaagcacagg cgggttcact gcatggtttg ctgcaactag
     5761 ccacagcagc tcccggtttg ctgatccctc ttttcttaca tcatgtgaaa gatcagcgtg
     5821 gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc tgttgggctc tgctttatgc
     5881 cagcgcacgc gatcacctgg actctgcttt tcggttttgg ttccggcgca acaatgatac
     5941 tggggttgac gttcattggt cttcgggcaa gttctgcgca tcaggcggcg gcactctcgg
     6001 ggatggcaca atccgtcggg tatttgctgg cagcctgtgg gccgccgctg atgggtaaaa
     6061 tacacgatgc taacggtgac tggtccgtac cactgatggg tgttgccata ctttcactac
     6121 tgatggcgat tttcggactt tgcgccggga gagacaaaga aattcgctaa tatccggtgc
     6181 tatagtgacg tgacaaatca tgcgtgaaag ggagaacaaa cacgatgaat attcagtgca
     6241 aacgcgttta tgatccggct gaacagagcg atggttatcg cgtactggtc gaccgcctct
     6301 ggccgcgcgg tatcaaaaaa accgatttag cccttgatga gtgggataaa gaaatcacgc
     6361 cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca acctttcgcg
     6421 agcaatatct tgcagaactg gcgcaacacg agcaagaagg aaagcggctg gcggacatcg
     6481 ccaaaaaaca gccgctgacc ctgctctacg cagcaaaaaa caccacgcag aaccatgcgc
     6541 tggtgctggc cgactggcta cgtagcttgt gattttagta cagcatccgg cggttatttt
     6601 tcaccagccg gatggtcccg tcgccacaat gcccattcat caatcgtttc accgcccggt
     6661 aatttgcaat tgttgctgac cccttgcgct gtctgcactg gaatgagcgt cccgcccttc
     6721 tgctggcaat agaccgacgc cggatttgcc ataccaatct gcggcggttt aggtgcttct
     6781 ggctgagaag aggttgaaca accagccagg accagcaagc aaggcaaagc aaaactgata
     6841 attttcattt attgatctca cacatttatc tacgcttaga gtatcgcggt atcgttttgt
     6901 tttgcagcac tatttttatt acattcactc aaaacatatt acgtcttgtt tcatctttgt
     6961 tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag catttacccg ccgtatcctg
     7021 gagttgtttc gtgtcagatc agattatcgc ccgcgtctcg caatcccttg ccaaagaaca
     7081 gtcactagaa agtctggtcc gacagcttct ggagatgctg gaaatggtca ctgatatgga
     7141 atcaacctac ctgaccaaag tggatgtcga agcgcgcctg cagcatatta tgtttgcccg
     7201 taacagccag aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt actcgttatg
     7261 caaacgcgct attgatgaaa actgcttttt cagcgatgaa gtccctgacc gttggggcga
     7321 ctgtattgcg gcacgcaatc ttggaatcac cacatttctg agtacaccaa ttcacttacc
     7381 ggatggatca ttctatggca cgctttgcgc cgccagcagt gagaagcgcc agtggagtga
     7441 acgcgcggaa caggttttac agttattcgc cggactgatt gcacaatata ttcaaaaaga
     7501 ggcgttggtt gaacagctgc gcgaagccaa tgccgcgctg attgcgcaat cgtataccga
     7561 ctcgttaacc gggctaccga atcggcgggc gatttttgaa aatctgacga cgctgttttc
     7621 tctcgcccgg catcttaacc ataagataat gatcgcgttt atcgatctgg ataacttcaa
     7681 attaatcaat gatcgttttg gtcataatag tggcgatctg tttctcattc aggttggcga
     7741 gcgccttaat acgctccagc aaaatggcga agttattggt cgtctcggcg gtgatgagtt
     7801 tttggttgtt tcactaaata acgagaatgc ggatatttcg tcgctgcgag aacgtattca
     7861 acagcaaata cgtggagaat atcacttagg tgatgttgat ttgtattatc ctggtgccag
     7921 tcttggcatt gtagaagtcg atcctgaaac gaccgatgca gacagtgccc tgcatgctgc
     7981 cgatatcgcg atgtatcagg agaaaaaaca caaacagaaa acaccttttg tcgcgcattc
     8041 agcgccacat tcctgaggcg tattcacatc cttttgattg gtgataacat gcgaatcggt
     8101 attatttttc cggttgtaat ctttattaca gcggtcgtat ttttagcatg gttttttatt
     8161 ggcggctatg ctgccccggg agcataaaga tgaaaaaaac aacgattatt atgatgggtg
     8221 tggcgattat tgtcgtactc ggcactgagc tgggatggtg gtaacgtcac ctctaaaaaa
     8281 tagcaaaggc tgcctgtgtg cagcctttgt gcaatttaag cgttaacttt taatcttcct
     8341 gtagataaat agcacgacaa tcgcaccaat aacggcaacc acgaagctgc caaaattgaa
     8401 gccatcgact ttaccaaagc caaacagcgt gctgatccat ccgccaacta cggcaccgac
     8461 tatccccagc aggatagtca taaagaatcc accaccatct ttacctggca tgatccactt
     8521 cgccagaata ccggcaataa gcccaaaaat aatccatgac agaatgccca ttgtttcctc
     8581 acttatctgt tttgcattag cgggttagtc gctgataaaa agcatagcac aacatcggga
     8641 gggcaagatt tgtgacgagc atcacggagg atttttttgc ggatggcaca gaaattgcgc
     8701 catcaacgat cagtgataat taccaaccac aaacatcatg ttcgttttcc gtgtcttgac
     8761 atcctccacg ccctgaatga cgaggatccc tgctacgttc aggctgtcgc ctgaatcatt
     8821 tcggtgggtt cctgcttcaa cgggcggcct gactgcacca tctctccaca ggcaagaacg
     8881 gcgtgccccg ccgctaaaat gttacgagcg ccgtttacat cggcgttcgc tgtatatcca
     8941 catacctggc atctgaattg actttgtgac aggcgatttt ctttcgctgt atgaccacag
     9001 cacgcgcaac gctggcttgt gtacgctggc ggaacagcaa gcacctgacc gccactccag
     9061 agctgcttgt actcaagctg gcggcgcatt tcataccagc cctgatccag tatcgaacgg
     9121 tttaaacctg attttgcccg gacattgcgc cccggctggc tgaccgtacc cgctgctgac
     9181 tttgacatgt tgctgacctt caaatcctca atgactatca ttgcgtggtt tttgctgacg
     9241 gtcgttgtga ctttatgaag gtagtccctg cggatatttg cgatacggga atgcagtcgc
     9301 tgtattttgc gtttctgctt ctgccagtta ttgctgaatt tgaccttgcg gcttaactgt
     9361 cgctgaagtc tcgccagcgt cttctggttt ttctgaaaac tgtttacagg cccaaagact
     9421 gtgccatctg acagcgtggc gagtttagcc acgccagcat ccagcccgat cattgatgct
     9481 gaagggtgaa ccggagttga tacttcactt tctgtctgaa tactgatgta ccacttaccg
     9541 caggactggc tgaccgtgac atttttcaca acacccgtga cctgacggct attccggtag
     9601 cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag ctttacgccc
     9661 tgcgggtagc ggaatgcatc attctgtccc cgctttttga atcggggaaa agcagcccgc
     9721 ttc
//
LOCUS       DAZFJF010000156         9553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFJF010000156 DAZFJF010000000
VERSION     DAZFJF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.157"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..211)
                     /gene="cspA"
                     /locus_tag="ACHKAU_004314"
     CDS             complement(<1..211)
                     /gene="cspA"
                     /locus_tag="ACHKAU_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO5533263.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(492..782)
                     /locus_tag="ACHKAU_004315"
     CDS             complement(492..782)
                     /locus_tag="ACHKAU_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO5533264.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1216..1926
                     /locus_tag="ACHKAU_004316"
     CDS             1216..1926
                     /locus_tag="ACHKAU_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO5533265.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1976..2950)
                     /gene="ghrB"
                     /locus_tag="ACHKAU_004317"
     CDS             complement(1976..2950)
                     /gene="ghrB"
                     /locus_tag="ACHKAU_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO5533266.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3054..3713)
                     /locus_tag="ACHKAU_004318"
     CDS             complement(3054..3713)
                     /locus_tag="ACHKAU_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO5533267.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3866..6199
                     /gene="bisC"
                     /locus_tag="ACHKAU_004319"
     CDS             3866..6199
                     /gene="bisC"
                     /locus_tag="ACHKAU_004319"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO5533268.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6168..6608)
                     /locus_tag="ACHKAU_004320"
     CDS             complement(6168..6608)
                     /locus_tag="ACHKAU_004320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5533269.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6605..7168)
                     /gene="tag"
                     /locus_tag="ACHKAU_004321"
     CDS             complement(6605..7168)
                     /gene="tag"
                     /locus_tag="ACHKAU_004321"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO5533270.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7326..8024
                     /locus_tag="ACHKAU_004322"
     CDS             7326..8024
                     /locus_tag="ACHKAU_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5533271.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8253..9455
                     /locus_tag="ACHKAU_004323"
     CDS             8253..9455
                     /locus_tag="ACHKAU_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5533272.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2253 a         2347 c         2487 g         2466 t
ORIGIN      
        1 acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca
       61 ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag aagtgtacga
      121 acacgtcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc
      181 attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa ttaagtgtgc
      241 ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt gcactacgag
      301 gggtatcaac gataactctt gaagggactt gccttactac actgctttaa tggtctgtac
      361 gtcaaaccgt tgatgtgcat taagccacgc attggcgggt gatgcaacaa ttatttttca
      421 tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag
      481 tggataaaag tctattccat caactgctta cttaatgccg ggttggcttg aatcaaacgc
      541 atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc ctttaccatg
      601 gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaaccctgt cccttttcgt
      661 aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca
      721 tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc tttatattcc
      781 attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct cattaagaat
      841 agtcgggaaa agcagacaca gcggggtgaa gtcagggcat gctctggcta tgattcgatt
      901 cagccagtca tttagcgtga cttatagcca cctaacgatc tgataaatgc cgatatatct
      961 cttaccactc ctggtttgta aagatttcga aaatctaacc ttctgcgaag gcgtaaatct
     1021 ctggctacaa aaaaagcaac ggcactatca ttttttaaca atgaaacaac aaattaacct
     1081 tgtttcattt tctccatgat tttaccagga agattacgta gaaagggtat cctgcaagcc
     1141 tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt
     1201 gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc gcctcttgcg
     1261 gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga tcagcgtaaa
     1321 gcgtttatcg acttcctgca gaatacagtg atgcgtagcg gtgaacgtct accaaccctg
     1381 actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat tctgtatggt
     1441 tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggtcgt agacagcgtt
     1501 aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg tgaaatgaac
     1561 ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga agttgcaggc cgatgctgcg
     1621 cactctgcgc taaaacagac cgatgatttg aaaccggtct ttgatcaggc attcaccaaa
     1681 gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggctgcaca aactttcacg
     1741 caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt gagctttgtc
     1801 gcaaatggca tccagttccc gacttcacag caggcaagtg aatataacaa actgattgcg
     1861 ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt tactgcaacc
     1921 caataaagag taaaagcccg agcgattatc gggctttact tcacgcagtc gcggcttagt
     1981 ccgcgacgtg cggattcaca cagttcttct caacctttcc ttgtaacgca tcaatcaaat
     2041 tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat ccaatatgcg
     2101 gtactgcgac gacgttggcc attgagagca acggcgaatc tacggacagt ggctcttgtt
     2161 cgaagacatc cagcccggcg gcgtgaattt cgcctttctg caatgcagca atcagtgcat
     2221 tttcatcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat ttcattttgg
     2281 cgaattgttc tgcgccaaac agatgatgcg tctcatcagt taacggcagg atcaggcaaa
     2341 cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg aagcgttctt
     2401 ctgcttcttt atggtggcgg cgcgcgttat agaggatggg catgttgaag ccaaagtgcg
     2461 cacgttgtgc cagcgccata ccgatccgtc ccatcccgac aatgcccagt gttttatggt
     2521 gaacgtcagt gccgtaccag tccgggccta tgctcgcggt ccattcgcct gcttttaccc
     2581 gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc agcgtatcgg
     2641 cgacggtttc tgttaatacg gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg
     2701 catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc agtttcggca
     2761 ttttttccag caatgcggca tcaacattct cgtttgaacc cagtaaacct tcagcttcgg
     2821 caaaaattgc tgcattttgt tcgacggttt gtgggctgag gtttgccacc tggtgaacgg
     2881 tgaaatgctc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag aggataacgg
     2941 acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg aaatatgatg
     3001 aaggaaaact gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag ggattacagc
     3061 gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt gctgttgctg
     3121 gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc cacgccctgg
     3181 gtgatcaacg cgctggcaac ggaatccgca cgttgctggg agagacgcat gttcaggtcg
     3241 tgaccacccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg atactctttc
     3301 agcaccattg ccacgccggt cagggtgtta gcgcccgcag gtttcagggt cgcgctgctg
     3361 ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct gcgggttacg
     3421 ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc catgtaataa
     3481 cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa tcagcgcgcc tttaccgcga
     3541 tctttcttcg aagaagagag cgcaccaata cccgcgccca cgagagagcc cagacctgcg
     3601 ccgatagcag atttacctgc ttcgcgttcg ccggtgtaag ggttagttgt gcagccagat
     3661 accgccagag cgccactcac tacggcggca ataagataaa cacgtttctt cattgttaat
     3721 ccttaataac ctttttattc tttgccacgg gttccgtggc gggagattat gccgcgtgaa
     3781 catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac tgctcaatac
     3841 atctaacctt tcaggagttt tcggtttggc caactcatcc tcacgatatt ccgttctgac
     3901 tgccgcccat tgggggccca tgctggttga aaccgacggc gaaaccgtgt ttagctcgca
     3961 tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg accaggttca
     4021 cagcaataca cgggtacgat ttccaatggt gcgcaaaggc tttcttgcgt caccggaaaa
     4081 tccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg aggcgctgga
     4141 tcttattcac caacaacata aacgcattcg tgaggcttat ggtccggcat cgatttttgc
     4201 tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat tattacaacg
     4261 ctatatggcg ctggcaggcg gttataccgg gcatctgggg gattattcga ccggcgcggc
     4321 acaggcgatc atgccgtatg tcgtgggtgg tagcgaagtt tatcaacagc aaaccagttg
     4381 gccgctggtg ctggaacata gcgatgtcgt ggtgttgtgg agtgctaacc cactcaatac
     4441 gctgaaaatt gcgtggaatg catctgatga gcaggggctt tcttactttt ccgcactgcg
     4501 tgacagcggg aaaaagctga tctgtattga tccaatgcga tcggaaaccg tcgatttctt
     4561 tggcgataaa atggagtggg tggcaccgca catgggcacc gatgttgcgc tgatgctggg
     4621 gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctga cgcgttgcac
     4681 cacaggttat gccgtcttcg cctcttattt gctgggcgag agtgacggaa tagcgaaaaa
     4741 cgccgaatgg gcggcagaga tttgtggggt taacgcagcg aaaatccgcg agctggcggc
     4801 tcttttccac caaaacacga ccatgctgat ggctggctgg ggaatgcagc gccaacagtt
     4861 tggtgagcaa aaacactgga tgatcgtcac gctggcggca atgttggggc aaatcggcac
     4921 acccggcggc ggttttggtc tttcttatca ttttgccaat ggtggtaacc ccacgcggcg
     4981 ttctgcggtg ctctcttcca tgcagggcag cttgccgggt ggctgcgatg cggtggataa
     5041 aatccctgtt gcccgcattg ttgaagcact ggaaaaccct ggtggcgcat atcaacacaa
     5101 cggtatgaac cgacatttcc cggatattcg ttttatctgg tgggcgggcg gtgccaactt
     5161 tactcatcat caggatacca atcgtctgat ccgtgcctgg caaaaaccgg agctggtggt
     5221 gatctctgaa tgcttctgga cggcggcggc gaaacacgcg gatatcgttc tgcctgcgac
     5281 tacctctttt gagcgtaatg atctcaccat gaccggtgat tacagtaatc agcatctggt
     5341 gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt aatgattttg atgtttttgc
     5401 cgagttgagt gaacgttggg agaagggcgg ctatgcgcgg tttacggaag gaaaaagtca
     5461 gctgcaatgg ctggaaacgt tttataacgt tgcccggcag cgcggggcaa gccagcaggt
     5521 tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga tgccggaaaa
     5581 cccggacagc gagcggttta ttcgcttcgc tgatttttgc cgcgatccgc tggcgcatcc
     5641 gttaaaaacc gccagcggca agattgaaat cttctcacag cgtattgccg attacggtta
     5701 cccggattgc cctgggcatc caatgtggct ggagccggac gaatggcagg gcaatgccga
     5761 accagaacag ttgcaggtac tttctgccca tccggcgcac cgcctgcaca gccagctgaa
     5821 ttacagttct ctgcgcgaat tgtacgcggt agcaaaccgt gagcctgtca ccattcatcc
     5881 tgacgatgct caggcgcgcg gcataaccga aggggatatg gtgcgggtgt ggaactcccg
     5941 tgggcagatc cttgccggag cggtcattag cgagggaatt aaacctggcg tgatttgcat
     6001 tcacgaaggg gcatggccgg atctggattt aaccgctgac ggtatttgta aaaacggcgc
     6061 agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct gtgcgggtaa
     6121 tacggcgctg gcatggctgg aaaaatacaa cggtccggaa ctgacactta cagcgtttga
     6181 accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca tgcgcaatcg
     6241 acaatgtgaa aaccctgcgc acggtaaaaa tctatcgccg gttgattttt ttgataaacc
     6301 tccagcatca gatggggata gcgctgctgc acatactgca tcagcgcctt accaataccg
     6361 cgtctgacgg ccttcggtgc gacaaacatt gctgccagaa atcggccttc cataatgctg
     6421 acaaaaccga gaagcttacc gtcctcttcc cagacccagt tttgcgcgtt ggcaagatag
     6481 gcatcccgca ccagcggaat gcagtcacgc cagtaattcg cttttataaa gggatgcccc
     6541 caggttgtac tttccagcca cagttcgagg atcgcgggga gttctgaacg ttgcgcttcc
     6601 cgaatcatgg tttatttccc ggatagcaac agcagccaac cacatgatca ttcaccagcc
     6661 cacatgcctg cataaaggag taacagattg tggtgccgac aaacttaaaa ccacgttttt
     6721 tcagtgcctt agatagggcg tcggaggcgg acgtagatgt gggaatttcg ctcaacgttg
     6781 tggcttgtgt cacctgtggc tgatgattta caaacgacca gacaaagtcg gcaaacggtt
     6841 cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc tgaattttcc
     6901 ctcgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct tcctgcattg
     6961 ctgcgacctt caccggatcg aactgatgaa agcaggcgcg atagttttcg cgttttttga
     7021 ggacggtgat ccacgataac ccagcctgct gcccttcaag gcagatcatt tcgaacagtt
     7081 ttttactgtc agtttcaggc acgccccatt cattatcatg gtaggcaata taaagcgggt
     7141 cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg atcgtaaaaa
     7201 tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca gggctactat
     7261 gcccgatatc cttcatgcaa tgacaataat attgccgaat tcggctcttc cctggagtcg
     7321 tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctg gcgagcgtgg
     7381 taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat atcgttgata
     7441 cgcaacccgg acattccaca gagcgttaca cctgggatag tgatcatcaa cctgattaca
     7501 acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg gaagtcaatc
     7561 tggcggaaga aactcctgtg gatgtgacca gcagtatgag tatgggctgg aattttcctt
     7621 tatatgaaca ggttacaacc ggcccggtcg cggcattaca ttacgatggc acaaccacct
     7681 cgatgtataa cgagtttggc gacagtacta ccacgctgac cgatccgtta tggcatgcca
     7741 gcgtgagtag cttaggctgg cgtgttgact cccggcttgg cgatctccga ccctgggcgc
     7801 aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca ggcctgagcc
     7861 ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc gctgatatgt
     7921 tgctcaatca aaatattgcc gcctatgccg cgctaactca ggcagaaaat accactaata
     7981 atagcgacta tctgtatacg atgggggtta gcgccagatt ttaacgtaac agtcacaatt
     8041 gaaaccattg aataacaaca gttgtggcga tagtgggtgc taacttacca aataataaat
     8101 ttggtgaata attgtcgcgt cattcattcc tgaactaagg catttcattc cgttctgatg
     8161 gcatttcatg ccgtttttcc ccaggcataa aatgcacttc gttatggttg tcggcagaga
     8221 tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat cagcgtaccc
     8281 gctggctgac actcatcggt actatcatta cccagtttgc actggggtcg gtttatacct
     8341 ggagcctgtt taatggcgcg ctttctgcca agctggatgc gccggtaagc caggtcgctt
     8401 tctctttcgg cttgttaagt ctggggctgg caatttcgtc ttctgttgcg ggcaaattgc
     8461 aggaacgttt tggcgttaaa cgcgtcacca tggcttccgg cattttgctg ggattaggct
     8521 tcttcctgac cgcgcattcc aacaacctga tgatgctgtg gttaagcgcc ggtgtgctgg
     8581 tcggtctggc ggatggcgcg ggttacctgc tgacgctctc taactgcgtg aagtggttcc
     8641 cggagcgtaa aggtctgata tccgcgttcg ctatcggttc ttatggtctg ggtagcctgg
     8701 gtttcaaatt tatcgacacg cagctgctgg aaacggtggg tctggaaaaa acctttgtga
     8761 tttggggagc gattgcgctg gtgatgattg ttttcggcgc aacgttaatg aaagacgcac
     8821 caaaacagga agtgaaaacc agcaatggtg tggtggagaa agattacacg ctggcagagt
     8881 cgatgcgtaa accgcagtac tggatgttag cggtaatgtt cctgaccgcc tgcatgagcg
     8941 gcctgtacgt gattggggta gcgaaagata tcgcccaaag tctggcacac cttgatgtgg
     9001 tttccgcagc caatgcagtc actgttattt ccatcgccaa cctttcaggt cgtctggtgc
     9061 tgggtattct gtctgacaaa atcgcccgta tccgtgttat caccattggt caggtgatat
     9121 cgctggtggg tatggcggcc ctgctgtttg caccattgaa tgcagtgacg ttctttgcag
     9181 cgattgcctg cgtggcattt aactttggcg gcactattac cgtctttccg tcactggtca
     9241 gtgagttctt tggcctcaat aacctggcga aaaactacgg tgtgatttac ctcggtttcg
     9301 gtatcggtag catttgtggt tcgattatcg cctcactgtt tggcggcttc tatgtgacct
     9361 tctacgtgat tttcgccctg ctgattctgt cattggcgct ttctacgaca attcgtcagc
     9421 cagagcagaa agtattgcgt gaggcgcata tttagaaata tgttgctact gaaataaatg
     9481 cgaggaggga gacttcaaag tctccctttt tttatggacg catattaaaa gaacaataat
     9541 atttacacca caa
//
LOCUS       DAZFJF010000157         9463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFJF010000157 DAZFJF010000000
VERSION     DAZFJF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.158"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(135..1622)
                     /gene="yeeO"
                     /locus_tag="ACHKAU_004324"
     CDS             complement(135..1622)
                     /gene="yeeO"
                     /locus_tag="ACHKAU_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO5533273.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1783..1858
                     /locus_tag="ACHKAU_004325"
     tRNA            1783..1858
                     /locus_tag="ACHKAU_004325"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1816..1818,aa:Asn,seq:gtt)
     gene            complement(1896..2846)
                     /gene="cbl"
                     /locus_tag="ACHKAU_004326"
                     /pseudo
     CDS             complement(1896..2846)
                     /gene="cbl"
                     /locus_tag="ACHKAU_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(2948..3865)
                     /gene="nac"
                     /locus_tag="ACHKAU_004327"
     CDS             complement(2948..3865)
                     /gene="nac"
                     /locus_tag="ACHKAU_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO5533274.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4191..4266
                     /locus_tag="ACHKAU_004328"
     tRNA            4191..4266
                     /locus_tag="ACHKAU_004328"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4224..4226,aa:Asn,seq:gtt)
     gene            complement(4310..5257)
                     /gene="ldtA"
                     /locus_tag="ACHKAU_004329"
     CDS             complement(4310..5257)
                     /gene="ldtA"
                     /locus_tag="ACHKAU_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5533275.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(5319..6398)
                     /gene="cobT"
                     /locus_tag="ACHKAU_004330"
     CDS             complement(5319..6398)
                     /gene="cobT"
                     /locus_tag="ACHKAU_004330"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO5533276.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6410..7153)
                     /gene="cobS"
                     /locus_tag="ACHKAU_004331"
     CDS             complement(6410..7153)
                     /gene="cobS"
                     /locus_tag="ACHKAU_004331"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO5533277.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7150..7695)
                     /gene="cobU"
                     /locus_tag="ACHKAU_004332"
     CDS             complement(7150..7695)
                     /gene="cobU"
                     /locus_tag="ACHKAU_004332"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO5533278.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7847..8020)
                     /locus_tag="ACHKAU_004333"
                     /pseudo
     CDS             complement(7847..8020)
                     /locus_tag="ACHKAU_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7999..8187)
                     /locus_tag="ACHKAU_004334"
                     /pseudo
     CDS             complement(7999..8187)
                     /locus_tag="ACHKAU_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(9184..9463)
                     /locus_tag="ACHKAU_004335"
                     /pseudo
     CDS             complement(9184..9463)
                     /locus_tag="ACHKAU_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2598 a         2538 c         2018 g         2309 t
ORIGIN      
        1 cagtccgccg ttctaccgac tgaactacag aggaatcgtg agaacgaggc gaatattagc
       61 gatgcccacc cacaatgtca aagcctgttt tttaaatttg aaatcgtttg ctgaaataat
      121 ctgcattttg ttgtttattc cgacacaact ggcttttttt cacacttttg cggttcgggt
      181 cgagggtatt tccatagcca acgtccagta accattcgcc agtaaaacag cacggcccgc
      241 acagcccagt cggcaaacat tcccatccag acaccaacca caccccagcc aagcatgatg
      301 cccagcacat aaccgacgac aacccggcaa ccccacatgc tcaacataga aacccacata
      361 gcgtaacggg cgtcacgagc ccccttaaat ccagcgggta gcacccatga ggcggaccaa
      421 ataggcataa ataaagcatt tagccaaatc agaatcacaa cgacatgttt aacctgtgga
      481 tcctgggtgt aaaacgatgc cataaccccg gcaaagggag ccgttagcca ggcgatggcc
      541 gttaatccaa gagtggacag ccagaagaca tgccgcaact gaatctctgc ttgcgctatc
      601 tgccctaccc ctaaccttcg gcctgtaatg atcgtagaag cagagccgag cgcacttccg
      661 ggtaagttga taagagccgc aattgaaaac gcgataaaat ttccggcaat aacactggtc
      721 cccatcccgg caacgaacat ttgggttaat aaccgaccac tggtaaataa cactgattcg
      781 acactcgctg gaataccaat ccccatgact tcccagataa tgctaaaatt cagcggttta
      841 aaatagctct ttaacgaaat ccttagcgca ggattaaaac caatcgccag cacccacaaa
      901 attgcaactg cgccaatata acgagaaatg gttaaaccca acccagcccc gacaaatccc
      961 agtcccggcc aggagaaaag gccgtaaatc aatatgccgc taataataat attaagaata
     1021 ttcaggctac cgttaatcag tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg
     1081 ctaccaatca aagtgatggc aactgctgga taactgagta ccgtcagctc cagataagtc
     1141 aacgccagtg ctttaacttc tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg
     1201 ccaaaatgat gaataagcgt tgccaacagt acggcaaaca acgtcatgat caccaatgac
     1261 tgccgcgtcg ccaccctcgc tcgtcgtcga tcccgcttac cgagactaaa tgccacaacg
     1321 acagtagtac caagatcgat agcagcaaaa aaagccataa tgaccatatt gaagctgtcc
     1381 gccaatccca cgccggccat cgcatctttt cccagccagc tgaccagaaa agtgctcagg
     1441 acacccatca acaggacaca ggcattctcc atgaagatag gaacagcaag cggggttatc
     1501 tcgcgccaga acaacacttt gtagctcttg cgtttagcgt gccagcgagt gccgtgaaca
     1561 acctggcgta aagcagagga gatattcaaa gccgacctta attgcagaaa gtgaaaccac
     1621 atttcaaata atgagggaga atcagcaaag ctgcaaagat tttcgccaac aaattgtctg
     1681 caaatgcaac aaactgttga tagaaacggc aaacagttgg gaaatttaaa aatcgggttt
     1741 gacaaaagat ttttcgccgt taagatgtgc ctcaacagcg attcctctgt agttcagtcg
     1801 gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa
     1861 tttctgaaaa gcccgctttt atagcgggct ttttgctata tctgataatc aatttcctct
     1921 tcactgttct ccatcacctg gcgcttgata tcctcaactg acagtcctgc attacaaagt
     1981 tccagaaagc gccagacata gttacgttga agttgccctc gcttcagtcc caaccataca
     2041 gtattggcat cgaaaagatg ccgcgtatcc agacggatta aattctcttc ctcttgttcg
     2101 ccgctggatt gttcggcaac taatccaatc cccagcccaa gagcaacata ggttttaatg
     2161 acatcagaat cctgcgcact taatacaata tctgccagca aacctttgcg ggcaaatgcg
     2221 tcatcaatac gtgagcaccc cgtaatcccc tatcggtaag tgattaacgg ccacttcgct
     2281 attgattcca gcgtcaacgg tgtaatttgc gtcaagggat gatcgagtgg aacaagcaaa
     2341 ctatggtgcc aacgaaacca cgggaaggcg acgagctgcg ggtcattact caaacgctcg
     2401 ctggcgatac caatatcagc ttcgccattt tgcaacaacg tcgcaatttc ctgtggcgta
     2461 ccctggatta gctccagccg aacctccggg aaaagttcgc gaaaagcttt aatgacctca
     2521 ggcaagctat aacgtgcctg agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg
     2581 ttggtaaaca ggtctgcaag ccgacgaaca ttactggctt cattcagaat acgttctgca
     2641 atgaccagta atgctttgcc cggttcagtc atacccagca gtcgcttacc tcgtcgaaca
     2701 aatatttcga tgccaagttc atcccccagt tcccgaatat gacggctgac gccggactgt
     2761 gaggtaaaaa gcatattcgc aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg
     2821 attatcttta gttgttggaa attcacggta aactccgggc agctcagatt tcccgttatt
     2881 gttaaagtct aatgcctggc ataacaaata ataaaaaccc gcatcttatt ccatcccgat
     2941 ataacgctta gctcaccaat tgccactgcc ttttttccat cactggagaa ctaatcactg
     3001 acattaacaa ctctttcact gcctgtgcct gtggcgataa gttcgctctg gcgggtaaat
     3061 ttaatgacaa agagagactc atggaaggcg tggtaatgcg tgacatccac ccatttactg
     3121 cgccacataa cgaacgcgcg gccgattcgg gtaatactgc aacgcccatg ccgctggcaa
     3181 tcgctgcggt aagcgtggca atagactcaa tttcaccaat gacttttgcc gtgagccgcc
     3241 gtagggaaaa agcctcatca acacgaagtc taacagcact gtaatcactg gggagaaaga
     3301 ggttcatttg cgcaatagca ttaacatcaa cgctttgccc cgggcaatct tgagttccta
     3361 ccaggaaaag atcttctttc agcaaagcct gactggatac cccagccaca ggggaatgct
     3421 cataaatcac cgccatatcg agttggtgat ttatcagttt ttcgttaagc actgcaccgc
     3481 tattttcatg aagatagata acgatctccg ggaattcagc gcgaaccgct tgtaataagg
     3541 gcatggtgat agatgacgca gcggttcctg gcgcaaagcc aatcgagact tgccccgata
     3601 atgcctgacc aacgttatgc accgccagtt gggcctgctc acactgacgt aaaatggccc
     3661 gcgcatgggt atagagaatt tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa
     3721 tcaaaagttg ttgatttaac tcaccttcca gtgtggcaac ctgctggctg agcgctggtt
     3781 gtgcgatatg caatacttca gcagcctgag tcaggctacc aatatctaca atttttacga
     3841 agtatttcag gcgtctgaag ttcatgttgc ctccggtttt tgagaatcgg cccaagtgcc
     3901 gccattactt acaaccagac tgcaagatgc ttgccagttt tattttggcg ttgatgtaca
     3961 agctaaccaa ctgtcaaata agagattatg atagattcgt cattggctct tttcaccagc
     4021 agtcgcgttc ccctgcccta taaaaggagg gtatgcacca cgatggttca ttaccaataa
     4081 gattgaaatc tcatcacttt gttgaaattg gcagcaaaca aacaaaaaaa tgcatttcac
     4141 cctttgacat caccatgcac tgccattaat atgcgccccg ttcacacgat tcctctgtag
     4201 ttcagtcggt agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag
     4261 gagccaaatt caaaaaagcc tgctttctag caggattttt gctttctaat taccaacgct
     4321 cataaaacat ctgtcttgaa ccagaactaa tttgcacagg cattcccgat cgacgttgca
     4381 acgcagcatt tgcgcgattc acatcaactt cttgcccgtt gataaacgcc cgcaaagatg
     4441 gggttaccgg caatggcact tttcggtcag actcatattc tgcacgattg cgcgacagcg
     4501 gctcatgaac ttccagccag ttcgagccat ctggttcagt ggtgtatttt actggctggt
     4561 cgataatttg cacacgcgtt ccaacaggaa cattatcaaa cagatatttg atatcgtcat
     4621 tgcgcagacg aatacagccc tgacttaccc gaagtccaat accaaaattg gcattggtac
     4681 cgtggatggc atacaacctg ccaatataaa tcgcgtacag ccccatggga ttatcgggcc
     4741 ccgcaggaac aaatgcgggc aaactctccc ctcgtttcgc atattcgcgc cgagtgttcg
     4801 gcgttggcgt ccaggttgga gcttcttgtt tacgttcaac ggtagtcacc cagttacgcg
     4861 gggtttctcg cccagcctgg ccgataccaa taggaaagac ttccacagta ttactgtctg
     4921 gcgggtagta ataaagacgc atctcagcga cgttaaccac aatcccttta cgaacagtgg
     4981 cgggcaaaat cagttgctgc ggaatggtga gttgcgagcc agacttcggc aagaaaacat
     5041 cagcgcccgg attcgcttcc agcatgttac ttaacccttg cccgtattgt gcggcaaaag
     5101 tctccagcgg ctgggtattg tgatcaggaa cagttacagt aagcgactgc cccaccaaac
     5161 ggctaccctc tggaggtaat ggataagtta ccgccaggct agtatggctg gcaaaaagca
     5221 gagcaaatga gcaaagaata tttacacgac gcatcatgtc cctttcctat gtcgcgaaag
     5281 ctatccgtta agtatagctt ttatcagact tttcgttttt aactgttcaa atcagaagtc
     5341 gtattccccg gtagaacaat attactggca gcaagttcgc ccatgttgtt atatatcgca
     5401 caggcagctt cgatgatggg catcgccaga gctgcgccac tcccctcacc taaacgcatc
     5461 tccatattga gataaggctc cagccccaaa tgcgacagcg ctatacgcgc ccctttttct
     5521 gccgacaggt gagaaggaat gagatacggt ttgattgcag gagacatctg gcaggctgcg
     5581 agcgcagcag cataagaaag aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg
     5641 cctaacatca cgccagccat tccgaccaaa tcaaatcccc ccacttttgc cagaacatca
     5701 acgccatcct gcggatttgg ttgattcaac gtaatcgccc ggcgcacaac atcaatttta
     5761 ttagccagtt tatctgtcgg caggtttgcg ccaatcccaa ccacttcttc aggatcccgg
     5821 ccagtgattg tgctgactat tgccgctgcc ggtgtcgtgt ttgccatccc cagttcacct
     5881 acaccaaaca gcgtgacacc gttttttgcc agctcccgcg tataacatat gacgtccaaa
     5941 agcaactttt cagcctgacg gcgactcatt gccggagctg aagcaatatt gccgctacct
     6001 cgtgcgacac gcatgttgat aagcccgggg ataggctcag cactatcaat accaacatca
     6061 actacgtgga cgttagcgcc cgcttgtgct gccagcacac acacgccggt tgttccgcgg
     6121 gtcatatttt cagcctgaat ggctgtcact tcttttgggg aaatagcgac cccttcctcc
     6181 cagacgccgt gatcggcaca cataaccagt accgcttttt tgcccacatg cggtatgcca
     6241 ttcaaccccg gcattcccgc cagttgtatg gcaagcgcct ccagccttcc caggctacca
     6301 acaggtttga gtaacccgtc aatatgccgt tgtgcacgcg acatagcggc aggatcgata
     6361 gcagggatcg tattcagtaa atcggcaagt gtttgcatgt cacgtctctt tatagcagtg
     6421 ccagcaggaa gaccagttca ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc
     6481 cgcccagcgt acgtttgaga agctgaccga ggatgaaaat cgccaccatc gtcaccacca
     6541 tagcagccac accatgcata cccggcaata atacggcagc aaaaatagcg gctaagccga
     6601 gtgtgacgca ggtttgtcgc ccatcaattt tgccaatgaa cacattgcca agcccttctt
     6661 cccgggcata acgatgacga tacatcaata atgcggcagt cccgcggctg accgcacatg
     6721 ctgccgccag cgatgcaagg atcggttcgc cacgcagagc caactcgctt aataccagaa
     6781 tctttgccag tacaacaaaa atcaatgcca gaccgccatg ggtgcctaaa cggctatcac
     6841 gcatgatttc caacatgcga tcgcggctac gtgcagaaaa tacgccatca caggtatcgg
     6901 cgaggccgtc taggtggaat cccccggtca tcagcgccag cacaagtacg ctaaacagtg
     6961 ccgccagtgg tacgccacac catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc
     7021 caagcaataa tccaattaaa ggaaaagtaa taataccgcg agaataatgc tcgaaatcca
     7081 gtccctggga cctgcgacgc ggtacaggca gacgcgtaat aaatgagagc atcgcccaaa
     7141 ataatttact catttgattt ttactccaat acccgaaacc accagccata cttcatttgc
     7201 cgcagcggcc aactgctgat ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg
     7261 actctccggc acaatcccca ttcccacttc gttagtcact aatacaacct ttgcggggca
     7321 acgttggcag gcagcaatca acgactgaat ctcagcatta atcgcctgtt ccatcgcctg
     7381 ataatcccat tcatcagggt ctttatcgcc gccataatca aacaacagat tagtcaccat
     7441 tgtggtaacg cattcaagca acacaacctc attcgggtta atgtctgcat gaattaattc
     7501 atcaagatgt tgccagcgct ccactgtgcg ccagtgctcc gggcggcctt gccgatgatg
     7561 ttctatccgt gcagccatct catcatcaag gatttgcgag gtagcgatat acagaacctg
     7621 tgaagagtcc ccaataagcg cctctgcgtg gcgactcttc ccgctccgtg cgccccccgt
     7681 caccaaaatc atcataccga ctcctgattc aaatgtatga tggtattcat tgaacagaac
     7741 aatggatccc ccaccgttgc tgaccgcaaa acatttacgc gattaatact gcgcgtaata
     7801 taattaaaaa tccagcattc tcaatccatt acgaaataag atggcattac ggaatagcca
     7861 gactttctcg tgttaacgtc cgacaaaatg cgtcattgac agcggtttct gtggattgac
     7921 acatttcatt cagataatga attaatgcta ctgccggaac aaccagcaaa cgggcattgt
     7981 gttctgaaat ccaggctatt gattcaacgt cagcgacaga tctgcgctgt aaaagaactt
     8041 gtaaaacaac gtaatagaat tatccggaat ggtggcgact atgcactaga acaccacaat
     8101 ttcgctctct cggtaaggca ctgtcacagc attcaacaga atgtgacttg ccagatttat
     8161 tagcgccacc agatgtttaa ccgggtaacc acgacccagt cggggatata ttccatcttc
     8221 tcgctgacat catcaagaat aaaaaggttt atcacactca gagcaacgtt cttcagcagg
     8281 cgggataatt cttcaacatt tagaagatgt gttattaaag gcatacaaac tggacgtatt
     8341 gtattttctt ttggtggttg acctaaggta gcagtttatc ctgatgcgct gagatttctg
     8401 taatatctac gtcaacattc tcctcgaata gaaaatgcag ccccggcaag tgattcacat
     8461 tttttaacaa aacgttgtta gcgtgccatt ttcagaagct ctccaagaaa gcgaattctt
     8521 ttctcaaatt ctgcattctc agacacagct ttctactcca tggtaatggc ccaaatacga
     8581 agttgctcag gatcattagt ggtgctgaga agttaaataa ccatgcggag taccactcgt
     8641 catcacgtat ttatatatca tgaagctatt gttgtgtttt gtacatcagt agatatattc
     8701 tgatatactc cttttgctag acataacctt tcacctgctt gcaaagcttc tgtgttctga
     8761 cattgccaaa ttgttgcaat tctgtatcca gccttctttc agtcatagct tcgggccgcg
     8821 ataagactca ctgatctgac cctgattcct cttgcagact ttatagacca attaaaatgc
     8881 agtttctgca ggtcaacgtc tgaccatcat tgtcatcact ctggccatta gagtaacctt
     8941 ctgcattcat ccttttgtaa aaagtttatg tcagtatcag caattaaccg gacctgatac
     9001 tgatatgagt cttaccgcat atacggtcaa tttcagcaat taattacatt atccacgcca
     9061 aagtgtttgt catcgcaatg atggtacctt ctttcagaca ccattttttc aactccgttt
     9121 tccacggacc gcactcttat gtcaagagtg cggtgatcct acccacgtaa tatggacaca
     9181 ggcctaagcg aggttctggt tttcaaattg ttccggactg aggccgccac accaactgtg
     9241 ccgccgccac cgattgtaat cacattcgat ataattaaac accgttgccc gcattatttc
     9301 ccggctgata aagtgttctc catggataca ttccactttc agcgaatgaa agaagctttc
     9361 cacgcagatt atgccgcttc agttgcgcct gataatctgc tgaacagtac tggcctccac
     9421 ggtccgtgtg aacgataacg ttccggggcc tcttacgccg cca
//
LOCUS       DAZFJF010000158         9144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFJF010000158 DAZFJF010000000
VERSION     DAZFJF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.159"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(121..1146)
                     /gene="tdh"
                     /locus_tag="ACHKAU_004336"
     CDS             complement(121..1146)
                     /gene="tdh"
                     /locus_tag="ACHKAU_004336"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO5533279.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1156..2352)
                     /gene="kbl"
                     /locus_tag="ACHKAU_004337"
     CDS             complement(1156..2352)
                     /gene="kbl"
                     /locus_tag="ACHKAU_004337"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO5533280.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2627..3484)
                     /gene="yibB"
                     /locus_tag="ACHKAU_004338"
     CDS             complement(2627..3484)
                     /gene="yibB"
                     /locus_tag="ACHKAU_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO5533281.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3788..4720
                     /gene="rfaD"
                     /locus_tag="ACHKAU_004339"
     CDS             3788..4720
                     /gene="rfaD"
                     /locus_tag="ACHKAU_004339"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO5533282.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4730..5776
                     /gene="rfaF"
                     /locus_tag="ACHKAU_004340"
     CDS             4730..5776
                     /gene="rfaF"
                     /locus_tag="ACHKAU_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO5533283.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5780..6772
                     /gene="rfaC"
                     /locus_tag="ACHKAU_004341"
     CDS             5780..6772
                     /gene="rfaC"
                     /locus_tag="ACHKAU_004341"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO5533284.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6769..7977
                     /gene="rfaL"
                     /locus_tag="ACHKAU_004342"
     CDS             6769..7977
                     /gene="rfaL"
                     /locus_tag="ACHKAU_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO5533285.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8013..9143)
                     /locus_tag="ACHKAU_004343"
     CDS             complement(8013..9143)
                     /locus_tag="ACHKAU_004343"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO5533286.1"
                     /translation="MIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGY
                     SNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVH
                     NSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIE
                     IVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAH
                     KNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLV
                     VIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDI
                     LKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         2515 a         2149 c         2022 g         2458 t
ORIGIN      
        1 atgagagcag ctatataaga gatcagcgca attacgatat ctttattaca ctgacaggat
       61 tttacatatt taacggatta tcctcaggca aaagggctgg aataccagcc cttgttcgtg
      121 ttaatcccaa ctcagaatta ctttcccgga ctggcccgaa cgcatagcgt caaagccctt
      181 ctggaaatca tcgatagaga aacgatgggt aatgatcggc gagagatcca gacctgactg
      241 aatcagcgcc gccatcttgt accaggtttc aaacatctca cgaccgtaaa tacctttaat
      301 gaacaagcct ttaaagatta ctttggtcca gtcgatagac atatcggacg gtggaatacc
      361 cagcatcgca atacgaccgc cgtggttcat ggtatcgagc atggtacgaa acgctggcgg
      421 cgcgccggac atttccagac cgacatcaaa gccttcggtc atgcccagtt cagccatcac
      481 atcattaagg ttttctttcg cgacgttaac cgcacgggtg atacccattt tgcgcgccag
      541 ctcaaggcgg tattcgttaa catcggtgat caccacattg cgtgcaccaa cgtgtttcgc
      601 caccgccgca gccataatac ctatcggacc tgcaccagaa accagcacat cttcgcccac
      661 cagatcgaac gacagcgcgg tatgcacggc gttaccgaag ggatcaaaaa tggaagccag
      721 gtcgtcggag atattgtctg ggattttgaa ggcgttgaac gccgggatca ccagatattc
      781 ggcgaagcag cccggacggt taacgccgac gccgatcgtg ttgcggcaca gatgggtacg
      841 accgccacga cagttgcgac agtgaccgca ggtgatatgg ccttcgccag aaacgcgatc
      901 gccgattttg aagcctttca cttcctgacc aatacctacc acttcaccga catattcatg
      961 gcctacgacc atcggcaccg ggatggtttt ttgcgaccac tcatcccagt tatagatgtg
     1021 aacgtcagtc ccgcagatgg ctgttttacg gattttaatc agcagatcgt tatgcccgag
     1081 ttccggtaca ggaacgtcgg tcatccagat gccctcttcc gctttcagtt tggataacgc
     1141 tttcatctca catcctcagg cgataacgcc cagttgttta ccaatacgcg tgaatgcttc
     1201 tactgcacgc gtaatttgct caggggtatg cgccgcagac atctgggtac gaatacgcgc
     1261 ctgacctttc ggaacgaccg gatagaagaa accggtgacg taaatgccct ctttttgcag
     1321 ctcacgggca aatttctgcg ccactactgc atcgccaagc atgaccggaa taatggcatg
     1381 atcggctccc gccagggtaa agcccgccgc cgacatttgc tcacggaact ggcgcgcgtt
     1441 cgcccacaga cggtcacgca gttcgctgcc cgcttcgacc atctccagta ctttgatgga
     1501 cgcggcaaca atggccggtg ccagcgagtt ggagaacagg tacggacgag aacgctggcg
     1561 cagccactca accacctctt tgcgcgccgc ggtataacca ccagaagccc cgcccagcgc
     1621 tttaccaagc gtaccggtga taatatcgac ccggcccatc acatcgcagt attcatggga
     1681 accacgacca ttttcaccga caaaaccgac cgcgtgggag tcgtctacca tcaccagggc
     1741 atcgtattta tctgccagat cgcaaacgcc cttcagattg gcaatcacgc cgtccatcga
     1801 gaatacacca tcggtggcaa tcagcacatg acgcgcaccg gcttcacgcg cttctttcag
     1861 acgagcttca agctcctgca tatcgttgtt ggcatagcga tagcgtttag ctttacacag
     1921 acgcacacca tcaataatag acgcgtggtt cagtgcgtcg gagataatgg cgtcttccgc
     1981 gcccagcagc gtttcaaaca ggccaccgtt agcatcaaag caggaagagt agagaatcgc
     2041 atcttccatc cccaggaagg ccgccagttt ttgttcaagc tctttatggc tgtcctgagt
     2101 gccgcaaata aaacgcaccg aagccatgcc gaaaccgtga gaatccattc ccgcctttgc
     2161 cgccgcaatc agatcaggat gattcgccag cccgagatag ttgttggcac aaaagttaat
     2221 gacgtggctt ccatcagcca cagtgatatc tgcttgctgc gcagacgtaa taatgcgctc
     2281 ttctttaaac aacccttccg cccgtgcggt ttccagatcg ttggttaact gctgataaaa
     2341 ttctccacgc attgcgattc tccagacagg gcaaattcca gcacatatta cccaaactta
     2401 taggtcgcga cgagatgacg cgttaacact tctgcaaaat tcaggataaa taacgtgtaa
     2461 cttgatgtct ctcaacacct acagatagta gaaaaactgt acagttttgg ctgggcataa
     2521 tgatgattga ttttactgca ttgatataaa aagaaaaaat gatgccaaag catcattttt
     2581 cctatctcag ttggtatcaa aacgctaccc actaatggag ggaaacctat tttcttgaaa
     2641 ggaaaattct cagcgcctgc atttttgccc ctaaagtgtt actcctgaag cagcgaaaca
     2701 aatcgaacca ttttcctctc cccagatagt ttaggttgaa gagatcgggg cgtttgtaat
     2761 agcacatgac aaaaatgccc tggtcatcat ccacaatgtt attatttagt gttatttttt
     2821 gactttccag aactaattta tagaattctt tccatttatg ctgtgaacca acaattgcgc
     2881 ccccaataat atatacatga ttgccaatca tgaaatcaaa aacctgttgt tgagatgtga
     2941 cagtaagccc tttttttata gtgaaaagat gcatcttact ttcatcaaat gggaaatccc
     3001 aaattttcaa tcctcgagtt acatttggct tacggcaata gccaaaatct atccacgcaa
     3061 caagaggcgt tttcactaag cccatgttga tagctttatt tacaaaatag gccttaaggt
     3121 tacataccaa aacatactct ggtgaccagt actctgggtt ttttaattgt cgaggctcaa
     3181 gtctatttgt aaacgactca tctttttgaa ttttttcgat acgacttctg atatatctaa
     3241 atttcttttt tatatcgata acgataaccg ttgttggttt tccgttgcga atcgcttcaa
     3301 cccttggttt caaatcggga gaagtgaaaa taatcatctc attttcaagt gcggccagac
     3361 gctcaaagta actaaaataa acatcaactg aacgagctag tttttcacgg aaccctttat
     3421 tagcagtcca atcccctcta ccaatgtcaa aatatgcagt gataattgtg gtggatgatt
     3481 tcattactaa taagaccatt aatataaaaa aagaattata tcataaatat taacagaatg
     3541 tatactgtat attatccatt caaatgcttc cgttatttat catacatcgg aatattgata
     3601 ctaaagcact attcacatgc aaaaccaaca tccgccatga aggactagct aaaacccaaa
     3661 ctagtttgtt gcaattagca tccttgcacc tctatgtaaa gggctgaagg gattcggatg
     3721 tgatggtatg attacagaca ttcgtgtctg agattgtctc tgactccata attcgaaggt
     3781 tacagttatg atcatcgtta ccggcggcgc gggctttatc ggcagcaaca tcgttaaagc
     3841 cctgaatgat aaaggcatca ccgatattct ggtggtggac aacctgaaag acggcaccaa
     3901 gtttgtgaac ctggtggatc tgaatatcgc agactatatg gataaggaag acttcctgat
     3961 ccagattatg gctggcgaag agttcggcga tgtcgaagcg attttccacg aaggcgcgtg
     4021 ctcttccacc accgagtggg acggcaagta tatgatggat aacaactatc aatactccaa
     4081 ggagctgctg cactactgcc tggagcgtga aatcccgttc ctgtacgctt cttccgcagc
     4141 cacctacggc ggacgcacct ccgactttat tgaatcccgc gagtacgaaa aaccgttgaa
     4201 cgtctacggt tactcaaaat tcctgtttga tgaatatgtt cgccaaatcc tgccggaagc
     4261 gaactcgcag attgttggct tccgctattt caacgtttat ggaccgcgtg aaggccataa
     4321 aggcagcatg gcgagcgtcg ctttccatct caacacccag cttaataacg gtgaatcacc
     4381 gaagctgttc gaaggtagcg agaactttaa acgcgacttc gtctatgttg gcgatgtggc
     4441 agatgtaaac ctgtggttcc tggaaaatgg cgtttccggc atcttcaacc tcggcaccgg
     4501 tcgtgcggaa tccttccagg ctgtagctga tgctacgctg gcttatcaca agaaaggcca
     4561 gatcgaatac attccgttcc cggataagct gaaaggccgc taccaggcgt tcactcaggc
     4621 agatctgaca aatctgcgcg cggcgggtta cgacaaaccg ttcaaaaccg ttgctgaagg
     4681 tgtaacggaa tacatggcct ggctgaatcg cgacgcataa gagctctgca tgaaaatact
     4741 ggtgatcggc ccgtcttggg ttggcgacat gatgatgtcg caaagtctct atcgcacgct
     4801 ccaggcgcgc tatccccagg cgataatcga tgtgatggca ccggcatggt gccgtccatt
     4861 attatcgcgg atgccggaag ttaacgaagc tatccctatg cctctcggtc acggagcgct
     4921 ggaaatcggc gaacgccgca aactgggtca tagcctgcgt gaaaagcgct acgaccgcgc
     4981 ctacgtctta cctaactcct tcaaatccgc attagtgcct ttcttcgcgg gtattcctca
     5041 tcgcaccggc tggcgcggcg agatgcgcta cggtttactc aacgatgtac gcgtgctcga
     5101 taaagaagcc tggccgctaa tggtggaacg ctatatagcg ctggcctatg acaaaggcat
     5161 tatgcgcaca gcacaagatc tgccgcagcc attgttatgg ccgcagttgc aggtgagcga
     5221 aggtgaaaaa tcatatacct gtaatcaatt ttcgctttca tcagaacgtc cgatgattgg
     5281 tttttgcccg ggtgcggagt ttggtccggc aaaacgctgg ccacactacc actatgcgga
     5341 gctggcaaag cagctgattg atgaaggtta tcaggtggtt ctgtttggct cggcgaaaga
     5401 tcatgaagca ggcaatgaga ttcttgccgc tttaaatacc gagcagcagg catggtgtcg
     5461 gaacctggcg ggggaaacac agcttgatca agcggttatc ctgattgcag cctgtaaagc
     5521 cattgtcact aacgattctg gcctgatgca cgttgcggcg gcgctcaatc gtccgctggt
     5581 tgccctgtat ggtccaagta gcccggactt cacaccgccg ctatcccata aagcgcgcgt
     5641 gatccgtctg attaccggct atcacaaagt gcgtaaaggt gacgctgcgg agggttatca
     5701 ccagagcttg atcgacatta ctccccagcg cgtactggaa gaactcaacg cgctattgtt
     5761 acaagaggaa gcctgacgga tgcgggtttt gatcgttaaa acatcgtcga tgggcgatgt
     5821 tctccatacg ttgcccgcac tcactgatgc ccagcaggca atcccaggga ttaagtttga
     5881 ctgggtggtg gaagaagggt tcgcacagat tccttcctgg cacgctgccg ttgagcgagt
     5941 tattcctgtg gcaatacgtc gctggcgtaa agcctggttc tcggccccca taaaagctga
     6001 acgcaaagcg tttcgtgaag cgctacaagc agagaactat gacgcagtta tcgacgctca
     6061 ggggctggta aaaagcgcgg cactggtgac acgtctggcg catggcgtaa agcatggatt
     6121 ggactggcaa accgctcgcg aacctttagc cagcctgttt tacaattgta agcatcatat
     6181 tgcaaaacag cagcacgccg tagaacgcac ccgcgaactg tttgccaaaa gtttgggcta
     6241 tagcaaaccg caaacccagg gcgattatgc tatcgcacag cattttctga cgaacctgcc
     6301 tacagatgct ggcgaatatg ccgtatttct tcatgcgacg acccgtgatg ataaacactg
     6361 gccggaagaa cactggcgag aattgattgg tttactggct gattcaggaa tacggattaa
     6421 acttccgtgg ggcgcgccgc atgaggaaga acgggcgaaa cgactggcgg aaggatttgc
     6481 ttatgttgaa gtattgccga agatgagtct ggaaggcgtt gcccgcgtgc tggccggggc
     6541 taaatttgta gtgtcggtgg atacggggtt aagccattta acggcggcac tggatagacc
     6601 caatatcacg gtttatggac caaccgatcc gggattaatt ggtgggtatg ggaagaatca
     6661 agtagagtgt cgttctacaa gtatgtctct tgcagatttg ccagctcaaa ccgtttttca
     6721 aaatttaaat cttgaaataa taaccaataa gttgacatcg gagataagat gacctcaaca
     6781 ttatttttct ctctcgagaa aaaaaactgg atagcgtact ggaacagagc tctcgtattc
     6841 ttattcatta ccacctattt tttgggtggg attacaaggt ataaacatct tattgttatt
     6901 cttatgacaa taacgacaat cgtctatctc tgcaaacggc caaaacacta tctctcacta
     6961 tttaaaacat ttctttttgg tagtgttgcc atattaacta ttgctgcatt gctgtcactt
     7021 cttcaatccc ctgatgcagg tgctagcatg aaggaaattt tcaaagctat tattgagaat
     7081 actttactat gcacaatagc aataccgatc atattgagag acgagaaaag agaagatgtc
     7141 gaaaaaatcg ttttcttctc atttattagt gcgttgggct tacgctgttt ttctgaattg
     7201 attacctatt ataaggacta tcaacaaggg ataatgccat tcgcagatta tagacaccgt
     7261 agcatttctg actcgatggt ctttttattc cctgcattgt taaatctctg gcttatcaaa
     7321 tcagcaaaat accgcatttc ttttgtggtt ctaagcgtta tttttatttt tctgatatta
     7381 ggaactttat ccagaggggc ctggctttcc gtgttagtca ttggattaat atggattctg
     7441 atgtttaaac aatggaagtt actattagta ggagtaatgg ttgccatcat tgcattgtcg
     7501 gttattttca cacataagga gatgaccgca aagctaacgt ataaacttca acaaactaat
     7561 agttcttatc gctatgcaaa tggtactcaa ggcagcgcac tcgatctaat attagaaaat
     7621 cctgttattg gttatggtta cggtaacgtt gcatataaag atgtctataa taaacgtgtc
     7681 attgattatc cagaatggac ctttagacaa tcaatagggc cacataattt tgcgctattc
     7741 atctggtttg gcactggttt attagggctg gtaagtctta tgatgctata ctgtgcaata
     7801 ttgaaagagt gtataaaaaa tggcgtcaag aataaatatc gctcaccata taatgcatat
     7861 tatataatct tactatcttt tataggttat tttgttatcc gtggaaacgt agaacaaatt
     7921 gaaccaaatt tattaggcgt ttacgccggc ttattattag cgatgaaaaa caagtaaaaa
     7981 ataaaaaagg ctgcataatg cggccttttt atttattcaa accaattatg aataacctct
     8041 tcgaatcttt gagttacgcc atcccaactg tagtgttcaa acacaaagtc ctgaccttgc
     8101 tttgcaactg ctgttaattc aggattcgct aacgttttta aaatatcgct gcttatggag
     8161 tctgcagtca taggttcttt taaatggaat cccgtagtat tttctttgac aaattccgtc
     8221 atccccccac gcgtactcac taaaacaggc ttaccagcac ccatagcttc aattgccacc
     8281 atgcaaaatg gttcctggaa ctgggaagga atgacgacta aatctgccag tgggtaataa
     8341 caatgcatct tttcaggcgg tatgcttcct aacataatac aacgatcctt caaacgtttt
     8401 gcaatttccc tgacattgcg ttgatatgca cctttgtcac ttttactcat ttctgtataa
     8461 tcaccaatca caactaattt taaatttttg tgagcagcgg ccaatttttc gaatgattgc
     8521 atcaacagca atatgccttt gtcagggacg atacgcccag cataaaaaat aattttctct
     8581 tcaggggaga tattaagttc gcttctagtg atcggctgaa aattagattg ataggtttct
     8641 aaatcaattc catttggtac aatctcaata tctgcatttg ctaaataaga ttggtaatac
     8701 tttttcaagt ataaactagg cactatcatt ttaacatttt gctcaagcaa ttttggttca
     8761 aatgcattat gcatatgtat aaccactctt gcctgaggag ctcgcttacg aatttgagtg
     8821 tatagcttca tgctattatg aactacaata acactttctt tcgttatagg aaaatcatgt
     8881 gcaatattca atattctctg tgaatatgga agaggatcaa gtcttgtcca tttttgaaat
     8941 aaccgcttat atatgcgact gaatccaacc ctatgaatac tacaattatc attaacaaag
     9001 gtgtaatttg agtatcctgg atttttgata caaacaatac gatttggaaa attggtacgc
     9061 tgagcaacct gatacatcca cgtttcaaca gcagccgctc cgcgaggagg aattgaaaaa
     9121 ataggagtaa ccgtaaatat tatt
//
LOCUS       DAZFJF010000159         9124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFJF010000159 DAZFJF010000000
VERSION     DAZFJF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.160"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1181
                     /locus_tag="ACHKAU_004344"
     CDS             <1..1181
                     /locus_tag="ACHKAU_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5533287.1"
                     /translation="DNTGAEPADEAGSEQPEETAETVSPVQRLAQLTAELDALHNDRL
                     LVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLAIASLHKH
                     LLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKH
                     TVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEM
                     ADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALLARMEV
                     VPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRAENGARML
                     ESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESVTKDETVL
                     "
     gene            1178..1921
                     /gene="vasI"
                     /locus_tag="ACHKAU_004345"
     CDS             1178..1921
                     /gene="vasI"
                     /locus_tag="ACHKAU_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO5533288.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            1926..3338
                     /gene="tssA"
                     /locus_tag="ACHKAU_004346"
                     /pseudo
     CDS             1926..3338
                     /gene="tssA"
                     /locus_tag="ACHKAU_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            3447..6881
                     /gene="tssM"
                     /locus_tag="ACHKAU_004347"
     CDS             3447..6881
                     /gene="tssM"
                     /locus_tag="ACHKAU_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO5533289.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            6892..8341
                     /locus_tag="ACHKAU_004348"
                     /pseudo
     CDS             6892..8341
                     /locus_tag="ACHKAU_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            8268..8750
                     /locus_tag="ACHKAU_004349"
     CDS             8268..8750
                     /locus_tag="ACHKAU_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5533290.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            8794..>9124
                     /locus_tag="ACHKAU_004350"
     CDS             8794..>9124
                     /locus_tag="ACHKAU_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533291.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKI"
BASE COUNT         2179 a         2506 c         2577 g         1862 t
ORIGIN      
        1 aggataatac cggtgccgaa ccggctgatg aagctggcag cgaacagccg gaagagaccg
       61 ctgaaacagt ttccccggta cagcggctgg ctcagctcac tgccgaactg gacgccctgc
      121 ataacgaccg gttgctggtc tccccgcacg tcgataaaaa acagattgcg gcggtgattg
      181 ccgaatggac cggcgtgccg ctcaaccgcc tatcgcagaa tgaaatgtcg gtcatcaccg
      241 acctgacgaa atggctgggt gacaccatca aaggccagga cctggcgatt gccagcctgc
      301 ataagcatct actgaccgca cgcgccgacc tgcgtcgtcc gggacgcccg ctcggtgcgt
      361 tcctgctggc tggccccagc ggtgtgggta aaaccgaaac cgtcctgcaa ctggcagagc
      421 tgctctacgg cggtcgccag tacctgacca ccatcaatat gtccgaattc caggagaaac
      481 acaccgtctc gcggctgatt ggttcgcctc cgggctacgt tggctacggt gaaggcggcg
      541 tactgaccga agcgattcgc cagaaaccct actcggtagt actgctcgat gaagtggaaa
      601 aagcgcaccc ggatgtgctc aacctgttct accaggcgtt cgataagggc gaaatggcag
      661 acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt cttcctgacg tccaacctcg
      721 gctaccaggt aatagtcgag catgccgatg acccggaaac catgcaggaa gtactgtatc
      781 cggtgctggc cgacttcttc aaacctgccc tgctggcgcg tatggaagtg gtgccgtacc
      841 tgccgctgtc gaaagagacg ctcgccacca tcattgccgg gaaactggcc cgcctggata
      901 acgtgctgcg cagccgcttt ggcgcggagg tgattataga accggaagtg acggacgaaa
      961 tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg gtcatcgacg
     1021 gcaatatgct gccgccgctc tccctgctgc tgttgcagaa aatggcggcg aatacggcgg
     1081 ttgcccggat tcggttgtcg gcagtggacg gcgcatttac ggcagacgtg gaagatgctc
     1141 agaacgacga gtccgtcaca aaggatgaaa cggttttatg agcaggttgc gcgttttccg
     1201 gcagacagga tggtttatcg cgggcctgat gacgggctta cccgccactg ccgcgcccgc
     1261 agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc accacgaccc cgccggatgc
     1321 gacagccacg ttacaggcca tgcagtcctg tcggcgggag tccgcggcgc tggagcggct
     1381 tgactgctat gaccgcctgc tggcaccgct gtccccgtca gggtttgacg gggcgctggt
     1441 caaagccggt tttgtgggcg aggcctggac ccgcgccaca gagcaggaaa aacgccgcga
     1501 gggcaacacc acagaactgc tggtgacgca ggtgccgggg gagcgcccga cggtggtgat
     1561 aaccaccccg gctatcggtc atgttccgcc acgcccggtg ctgatgttca gttgcgtgga
     1621 caatatcacc cggatgcagg tggcgctgat gcaccctctg gatgtccatg atatcgccgt
     1681 cactctgaat gccgacaacc gggcattgcg cagccactgg tttgtgcgcg agaacggcac
     1741 cctgctggag tccagccggg ggttatccgg gattgatgaa attaagcaac tgttcggcgc
     1801 aaaaacgctg acggtggata ccggcgcaga caatgccgcc ggaaagctga cttttaacat
     1861 tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc tgccactggg cgggagaata
     1921 aaaatatggc aatggactta cgcgatccga atgtctggat atcgcacctg ctggaaaacc
     1981 tgccggaaga gaaactggca tcggcgctga aagatgacaa cccgaactgg gagtatatcg
     2041 acggcgaaat cgtcaagctg ggatcgctgg cccacagtca actggatatc ccggaactcc
     2101 agcgccgtgg gctggtcatt ctggcttcgg agagtaagga ttttcgtctg ctggcgcatc
     2161 tgctgagaac cctgcaacac gccggtgatc ctcatctcgc attgcgcctg ctggcgctgt
     2221 atgtggagca ttactgggcg gtggctgcgc cgcagaatat ggcgcataaa aaacgctttg
     2281 ccagccaggt cattaaacgc tttgaaacag gtattgaagt tttttcacag aacgccgcca
     2341 cagcgcagcg cgatgcgctg tcaggggaac tggcaaaact ggcgcaatgc tggcagtcac
     2401 ataacgcccc ggaactggca caggctactg atgatctttt tgccctgtac cagcgtgcgt
     2461 ttaatcgtgc ggcaccggct cctgtcccca ctccggcggc ctccggtagt tcaccacaga
     2521 ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc tccggctccc caaatcgtca
     2581 tcgacagtca cgacgacaaa gcctagcgcg acacgctgtt aaaagtggcg gccattttat
     2641 gtgaacgcca gccggattca ccgcagggct accgtctgcg ccgccatgcg ctttggcaga
     2701 acatcaccag tacgccccag gcggaaagcg atggccgtac tccactggct gcggtctccg
     2761 ccgatatggt ggcagattac catgctcagc tcggtagtgc cgatatggcg ctgtggcagc
     2821 aggtggagaa aagcgtactg ctggctcctt actggctgga tggtcactgc ctttctgcac
     2881 agacggcgct gcgtctgggt tacaaacagg tggcagacgc catccgcgat gaggtcatcc
     2941 gcttccttga gcgtctgccc cagcttaccg ggctgctgtt taatgaccac acaccgttta
     3001 tcagtgagca gacgaaacaa tggctggcag cttcgcccga cgccaaagtt gcaccggttg
     3061 cgcaaatcgg tgaggaatcg aaggcagcca gagcctgttt tgctgagcag ggtctggagg
     3121 cggcgctgcg atatctggac atgctacccg aaggcgatcc ccgcgatcag tttcaccgcc
     3181 agtaccttgc cgcacagttg acggaagagg cggggctggt acagcttgcg cagcagcagt
     3241 accggatgtt gttcaggatg ggattgcaga tgatggtggc agactgggag ccgtccctgc
     3301 ttgagcagct agaacaaaaa ttcacggcag aacaataaaa gagaacagga gtttttgtgt
     3361 tcagattacc aacaccccga ctactcagcg gactcaaatc agccctgcga ccggcgatgc
     3421 cccggtttaa agtctctgct ttctggctgc tgatactggc gtggattttt ctgctggtgt
     3481 ggatctggtg gaaaggcccg atgtggacgc tgtatgaaga acagtggctc aaaccactgg
     3541 cgaaccgctg gctggcaacg gcggcgtggg ggattattgc cctggtgtgg cttaccgtcc
     3601 gggtgatgaa gcgcctgcaa cagcttgaaa aaatgcaaaa gcaacagcgc gaggaagccg
     3661 ttgatccgct cagcgtggaa ctgaacgccc agcagcgtta tcttgaccgc tggctgctgc
     3721 gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca gttgccgtgg tatatggtca
     3781 tcggcccggc gggcagtggc aaaacgacac tgctgcgcga ggggtttccg tccgacatta
     3841 tttatgcccc ggagggcgca cgcggcgcag aacaacgcct gtacctcacg ccccatgtcg
     3901 gtaaacaggc ggtgatcttt gatatcgacg gcacactctg cgctcccgct gatgcggata
     3961 tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct gaaggaaaag cgcgcacgcc
     4021 agccgctgaa cgggattatt ctgacactcg atttacccga tctgctgacc gcagacaaac
     4081 gccgccgcga gcatctgtta cagacgctgc gcagccgctt acaggatata cgccagcatc
     4141 ttcactgcca gttaccggtt tacgtggtac tgacccggct tgatttatta cagggttttg
     4201 ccgccctgtt ccagtcactc aacagacagg atcgcgatgc gattctgggc gtcacgttca
     4261 cccgccgcgc ccatgaaaat gatgactggc gaacagagtt gaatgctttc tggcagacat
     4321 gggtggatcg aatgaatctg gcgttgccgg atctgatggt cgctcagact cacacccgcg
     4381 cgtctttatt cagtttttcc cgccagatgc agggaagccg tgaaccgctg gtgtcactgc
     4441 ttgagggtct gcttgatggc gaaaatatga acgtgatgct gcgtggtgtc tatctcacct
     4501 cttcgcttca gcgtgggcag atggatgata tattcaccca gtctgccgcc cgccagtacc
     4561 ggctgggcaa taacccactg gcgtcctggc ccctggtgga caccgcgcct tatttcaccc
     4621 gcagcctgtt cccgcaggca ttactcgcag agcctaatct ggcaacagag agccgcgcct
     4681 ggctgatacg ttcccgtcgc cgcctgacgg ttttctctgc cacaggcggc gtggcagcac
     4741 tgctgctcat caccggctgg catcactatt acaacggtaa ctatcagtcc ggcatcaccg
     4801 tgcttaagca ggccaaagcc tttatggacg tgccgcctcc gcagggggaa gatgactttg
     4861 gcaacctgca actgccgctc ctgaacccgg tacgcgatgc cacactggcc tatggcgact
     4921 ggggcgaccg cagccgtctg gccgatatgg gactgtacca gggacgacgt atcgggcctt
     4981 atgtggaaca gacctatctg caactgctgg agcaacgtta cctgccctcg ctgtttaacg
     5041 ggctggtcaa agcgatgaac gccgcgccgc cggagagtga agaaaaactc gcggtgctgc
     5101 gcgtaatgcg aatgctggag gacaaaagcg gacgtaacaa tgaggtggtg aagcagtata
     5161 tggcaaaacg ctggagcgaa aaattccacg gccagcgcga tatccaggca caactgatgt
     5221 cccatcttga ctacgcgctg gctcatactg actggcatgc agagcgtcag gcgggcgacg
     5281 gtgacgccat cagccgctgg acgccatatg acaagcccgt ggtatcagca cagaaagaac
     5341 tgagcaaact gcctgtctac cagcgggttt accagagcct gaaaacgcgg gcgctgggcg
     5401 ttcttcctgc cgacctcaat ctgcgtgacc aggtagggcc aacctttgac caggtgttta
     5461 catctgccga tgacaacaaa ctggttgttc cacagtttat cacccgctac ggcctgcaaa
     5521 gctattttgt aaaacagcgc gatgaactgg ttgaactgac agcgatggat tcctgggtac
     5581 ttaacctcac ccgcaatgtg aaatacagtg acgccgaccg tgcggaaatc cagcaccagt
     5641 tgaccgagca gtatatcagc gactacaccg ccacctggcg ggctgggatg gacaatctga
     5701 atatccgcaa ttttgagtcc atcggacaac tgaccggggc gctggagcag gttatcagcg
     5761 gcgaccagcc tttgcagcgg gcgctgaccg tgctgcgtga caacacacag ccaggcgtct
     5821 tttctgaaaa actctctgcc aaagaacggg aggaagccct ggcagagccg gattaccagt
     5881 tactcacccg cctcgggcat gaattcgccc cggaaaacag taccctggca gtacagaaag
     5941 acaaagaaag cacgatgcag gccgtgtatc agcaactcac cgagttgcac cgctacctgc
     6001 tggcaatcca gaacgcgcct gtaccaggga aatcggcgct gaaagccgtg cagttacggc
     6061 ttgatcagaa cagcagcgat ccgatattcg ccacccgcca gatggcaaaa acgctgcctg
     6121 ctccgctcaa ccgctgggtt ggcaggctgg ctgaccaggc ctggcatgtg gtgatggtgg
     6181 aggctgttca ttatatggaa gtggactggc gcgacagcgt ggtgaaaccg tttaacgagc
     6241 aactggcaaa taactatccg tttaatccgc gttctgcaca ggatgcctca ctggatgcct
     6301 tcgaacgctt ctttaaaccg gatggcatac tggatacctt ctaccagcag aacctgaagc
     6361 tgtttatcga taatgacctg agtctggagg atggcgataa caacgtcatt attcgcgaag
     6421 atattattgc gcaactggaa actgcgcaga aaatccgtga catcttcttc agcaaacaga
     6481 acggtctggg aacatccttt gccgtggaaa cggtatcgct ttcaggcaat aaacgccgca
     6541 gtgtactgaa ccttgacggt cagttagtcg attacagcca gggccgtaac tataccgccc
     6601 atctggtctg gcctaacaac atgcgcgaag gcaacgaaag taagctgacg ctcatcggca
     6661 ccagcggcaa cgcgccgcgc agtatcagct tcagcgggcc gtgggcgcag ttccgcctgt
     6721 tcggggccgg acaactgacc ggagtacagg atggcaactt taccgtgcgc tttagcgtgg
     6781 acggtggcgc gatgacctac cgtgtgcata ccgacacgga agataacccg ttcagcggtg
     6841 ggttgttcag ccagtttggt ctgtcagaca cactgtactg aggaggcagg gatgaatagt
     6901 aacgtactga cacaaactat cgttaccggc agtgacccgc gcgggctgcc ggaattcagc
     6961 gccatccgcg aggaaataaa caaagccagc cacccgtcac agcctgagct gaactggaaa
     7021 ctggtggagt cgctggcgct ggcgattttt aaagccaacg gtgtggattt acacaccgcc
     7081 acctactata cgcttgcccg gacacggaca cagggactgg cgggattctg cgaaggtgcg
     7141 gaactgctgg cggcaatggt aagccacgac tgggataagt tctggccgca gggcggcccg
     7201 gcgcgtactg aaatgctgga ctggtttaac tcccgaaccg gcaatattct gcgtcagcaa
     7261 atctcctttg cggaatccga cctaccactg atctaccgca cagagcgggc attgcagctt
     7321 atctgcgata agctccagca ggtggaactg aagcgcgttc cgcgcgtgga gaatctgctc
     7381 tattttatgc agaacacgcg taaacggctt ggaccccagc tgaagagtaa cactgagaac
     7441 gccgcacaga ccacggtcag aacgctgatt tatgccccgg aaacacaggc atcttccaca
     7501 ccagaagcgg tagtgcctcc cctgcccggc ctgcctgaga tgaaagtgga agttcgcagt
     7561 ctgacagaga atcccccaca ggccagtgtg ataaatcaag gcagtacggt aagagggttt
     7621 atcgcaggga tcgcctgttc agtggctgtc gcctcagcat tgtggtggtg gcaggtctat
     7681 ccggtgcagc agcaactgtt acaggttaac gacaccgctc agggcgcagc aacggtgtgg
     7741 atggcctcac ctgaactcga aaactatgag cgcaggctgc aacaacttct tgatacctcc
     7801 ccggtacagc cgctggaaac cgggatgcag atgatgcgtg ttgccgacag tcgctggccg
     7861 gaaagcctgc aacagcaaca ggcctcgaca caatggaatg aggcactcaa aacccgcgca
     7921 cagagtagcc cgcagttgcg tggctggttg cagacccgcc aggacttaca tgcttttgca
     7981 gatctagtga tgcagcgcga gaaagaggga ctaacccttt cctatatcaa aaatgtcatc
     8041 tggcaggcgg agcggggact ggggcaggaa acacccgttg agtctctgtt gacgcagtac
     8101 catgatgccc gtgcgcagaa gcagaataca gatgcgctgg aaaaacaaat taatgagcga
     8161 ctcgaaggcg tgttaagccg ctggctgctg ctgaagaata acgtcatgcc agaggcggca
     8221 accggtacca cggccgaaaa ataattattt aattaaagga gattatcatg gcaaatataa
     8281 gttatttatc tttgagtgga gagacacaag ggctaatttc agcaggttgc tcaacgcttg
     8341 attctgtcgg aaacaaagcg cagccagaac ataaagatca gatcatggtt tatgcgttaa
     8401 tgcattccat ctcaagaagt caaaatgtca atcatcatga attaattatc accaaaccgg
     8461 ttgataaatc ctcaccattg ctggcaaaag cacttagtga caatgaaaaa atggcaatat
     8521 gtgaatttat actctaccga accagtaaag cgggtattta ccagccttat tataaaataa
     8581 atctgtcaaa agcccgaata tcaagtattg attttgtcac accgcatgct gtcctggaaa
     8641 aagaactgga accacaggag agaattgcct ttatttatga agatatttcc tgggagcata
     8701 cgttagccgg tacgaatgca atgagtaaat ggcaggatcg tgtacagtaa cttactgtaa
     8761 taacataata aattactatt gcggagagat aaaatggctg gtttaaggaa taacaacaac
     8821 acacagaatg cacagtgggc tgattatgtg ggtgacattc ttcggggggc tcagccgata
     8881 aaccagctag tacctcagca cccttatctt aacgacgtcc cactcattga tgagttaagg
     8941 catcagaaca ctcatcatgt tattctcttg acacttgatg ttgcaaaaaa aatactcagc
     9001 ccaataacat catttgatta cattcatttt atcactacac atccttcagg tataaaagat
     9061 acactcgcct ggttagttaa cgcgggtaaa ttaatgactg agtttgatga caatgggaaa
     9121 atta
//
LOCUS       DAZFJF010000160         9120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFJF010000160 DAZFJF010000000
VERSION     DAZFJF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.161"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..516
                     /locus_tag="ACHKAU_004351"
     CDS             <1..516
                     /locus_tag="ACHKAU_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFO5533292.1"
                     /translation="NISEEALAWILDNAANVPVFVDPVSAWKCVKVRDRLNQIHTLKP
                     NRLEAETLSGIALSGRGDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGWSAPI
                     KTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIANV
                     ISLVENAECLN"
     gene            504..1442
                     /gene="psuG"
                     /locus_tag="ACHKAU_004352"
     CDS             504..1442
                     /gene="psuG"
                     /locus_tag="ACHKAU_004352"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO5533293.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1536..2786
                     /locus_tag="ACHKAU_004353"
     CDS             1536..2786
                     /locus_tag="ACHKAU_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5533294.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2854..3552)
                     /locus_tag="ACHKAU_004354"
     CDS             complement(2854..3552)
                     /locus_tag="ACHKAU_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO5533295.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            3682..4623
                     /gene="rihB"
                     /locus_tag="ACHKAU_004355"
     CDS             3682..4623
                     /gene="rihB"
                     /locus_tag="ACHKAU_004355"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO5533296.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            4723..5973
                     /locus_tag="ACHKAU_004356"
     CDS             4723..5973
                     /locus_tag="ACHKAU_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5533297.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(6029..7117)
                     /locus_tag="ACHKAU_004357"
     CDS             complement(6029..7117)
                     /locus_tag="ACHKAU_004357"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5533298.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(7121..7978)
                     /gene="nfo"
                     /locus_tag="ACHKAU_004358"
     CDS             complement(7121..7978)
                     /gene="nfo"
                     /locus_tag="ACHKAU_004358"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO5533299.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(8052..9101)
                     /locus_tag="ACHKAU_004359"
     CDS             complement(8052..9101)
                     /locus_tag="ACHKAU_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO5533300.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
BASE COUNT         2231 a         2183 c         2375 g         2331 t
ORIGIN      
        1 aatatcagtg aagaggcact ggcatggatt ctggataatg ctgccaacgt acccgtattt
       61 gtcgatccgg tttcagcgtg gaaatgtgtc aaagtgcgcg accgtctaaa tcagatccac
      121 actctgaagc caaaccgcct tgaagcggaa acactgagtg ggattgcgct gtcagggcgt
      181 ggagatgtgg caaaagttgc tgcctggttc catcaacatg gcctgaaccg actggtattg
      241 agcatgggcg gcgatggcgt ttattacagt gatatcagcg gtgaaagtgg ctggtctgcc
      301 ccgatcaaaa ccaatgttat taatgttacc ggagcgggcg atgccatgat ggcgggactt
      361 gcttcgtgtt gggtagacgg aatgccgttt gccgaatctg ttcgtttcgc acagggatgt
      421 tcgtcaatgg cgctctcctg tgaatacacc aataaccccg atttatcgat tgccaacgtt
      481 atatcgttag tggagaacgc agaatgtctg aattaaaaat ttcccctgaa ttattacaaa
      541 tttccccgga agtgcaggac gctttaaaaa acaaaaaacc ggttgtggcg ctggaatcga
      601 ccattatttc tcacgggatg ccgttcccac aaaatgccca gaccgcaatt gaagtagaag
      661 aaactattcg taaacagggc gcagtacctg ccactatcgc cattattggc ggcgtgatga
      721 aagtgggttt aagcaaagaa gaaattgaat tactgggtcg tgaagggcat aacgtgacca
      781 aagttagtcg tcgcgattta ccttttgttg ttgccgccgg aaaaaatggc gcaaccactg
      841 tggcttcaac gatgattatt gcggcgcttg ccggaattaa agtatttgcc accgggggaa
      901 ttggtggtgt gcatcgcggg gcggaacata ccttcgatat ttctgccgat ttgcaagaac
      961 tggcaaatac taatgtcacc gttgtttgtg ccggggcgaa atctattctc gatttaggat
     1021 taacaacaga atatttagaa accttcggtg tgccgttaat tggctatcag acaaaagcgc
     1081 tgcctgcgtt tttctgccgt accagcccat ttgaagtcag tattcgcctt gatagtgcca
     1141 cggaaatcgc tcgtgcaatg gcagtgaaat ggcaaaccgg tttgaacggc ggcctggtag
     1201 tggcgaatcc gatcccggaa cagtttgcga tgccggagga atctatcaat gcagccatag
     1261 atcaagccgt cgccgaagcc gaagagcagg gcgttattgg taaagaaagt acaccgttcc
     1321 tgctggcgcg cgttgctgag ctgaccggcg gtgacagcct gaaatccaat atccagctgg
     1381 tgttcaacaa cgccattctg gcgagtgaaa ttgccaaaga ataccagcgt ctcgcgggtt
     1441 aatttctgac gggcagaaag ccgtcgcccg tactcatcaa gcataccaaa acctggcgct
     1501 gacgccgggt ttactggcat gaagggaaaa tcattatgga tataatgaga agtgttgtgg
     1561 ggatggtggt gttactggca atagcatttc tgttgtcagt gaataaaaag agcatcagtt
     1621 tgcgcacggt tggagccgca ctgctgctgc aaatcgctat tggtggcatc atgctctact
     1681 tcccaccggg aaagtgggca gtagaacagg cggcattagg cgttcataaa gtgatgtctt
     1741 acagtgatgc cggtagcgcc ttcatttttg gttcgctggt tgggccgaaa atggatgtcc
     1801 tgtttgacgg tgcgggtttt atcttcgcct ttcgcgtact tccggcgatt attttcgtta
     1861 ctgcgctcat cagtctgctg tactacattg gcgtgatggg gctgctgatt cgcatccttg
     1921 gcagcatttt ccagaaagct cttaacatca gcaaaatcga atcttttgtc gcagtcacaa
     1981 ctattttcct cgggcaaaat gagatcccgg cgatcgttaa accgtttatc gatcgcatga
     2041 atcgcaacga gttgtttacc gcaatttgta gcgggatggc gtccattgct ggttcgatga
     2101 tgattggtta tgccggaatg ggcgtaccaa ttgactacct gttagcggca tcgctgatgg
     2161 cgatcccagg tggtattttg tttgcacgta ttcttagccc ggccaccgag ccttcacagg
     2221 tcacatttga aaatctgtcg ttcagcgaaa cgccgccaaa aagctttatc gaagcggcgg
     2281 cgagcggtgc gatgaccggg ctgaaaatcg ccgctggtgt agcgacggtg gtaatggcgt
     2341 ttgtcgcaat tattgcgctg atcaacggca ttatcggcgg aattggtggc tggtttggtt
     2401 tcgccaatgc ctctctggaa agtatttttg gctatgtgct ggcaccgctg gcgtggatca
     2461 tgggtgtgga ctggagtgat gccaatcttg cgggtagcct gattgggcag aaactggcga
     2521 ttaacgaatt cgtcgcttac ctgagtttct ccccatacct gcaaacgggc ggcacgctgg
     2581 aagtgaaaac cattgcgatt atctcctttg cgctttgtgg ttttgctaac tttggttcta
     2641 tcggtgttgt cgttggcgca ttttcggcta tttcgccaaa acgcgcgccg gaaatcgccc
     2701 agcttggttt acgggcgctg gcagcagcaa cgctttccaa cctgatgagt gcgaccattg
     2761 ccgggttctt tattggttta gcttgagttt cattgccgga tgtacgcgct tattaagtgt
     2821 tgcatccggc aatcgcgcta ttgcaaataa tttttactgc atcatcccgg agaatttatt
     2881 ctccgggtcc atctccagcg ccagtcctga caactgcttt ctgtttttaa ttagataccc
     2941 tttcttgctt ttaattaata aaccgtcgtg aatgaattgc gcgagaacat ataaaagatg
     3001 tcgataagaa acgcctaaat actctgccgc ttgcgtatgc ttttcgtgat aaagatcacc
     3061 ttcctgcgag agtaatataa atgctgccag gcgattcact aacggaaatg attgattctg
     3121 agttaaagaa acaatattac gataattttt atgacttaag gtgacgcaga gttttcgtaa
     3181 aaatagcgtg tcgtttaata accgcgggcg gtaatgtttc atagggagcg caaggcacca
     3241 acactcttca atagcctgca ccgcacgcgg ttcatggtct ttgtcgatta actcaatctc
     3301 gccaataaaa cagggggcgg caaagaaatc gatcagcgac acgcgaccat tagctagcgt
     3361 ggcgtaaagc ctggcgcggc ctcgcgtcag gtaaaacagc caggacggct gttgcccttc
     3421 ctgcacaatg tagtcacgcg ctaaaaaatg aaacagccgc gtatcggctg aaacatccgt
     3481 taaaaagcaa tccttaaacg cggattcact catcaattgc tgcttaagat cattattgtg
     3541 gatttctttc acgatgttta tccgctatga gatttctcat atttagcata cgtacgcggt
     3601 tggtaagatt caatcatcgg cccggtcgaa ttttctttgc tgtagaaaaa ataacgcaac
     3661 tggaaacaga ggaaataaac aatggaaaag agaaaaatta ttctggattg tgatccgggt
     3721 catgatgatg ctattgcaat gatgatggca gcgaaacatc cggcaataga tttattaggc
     3781 atcactattg tagcgggtaa tcagacgctt gataaaacat taattaatgg cctgaatgtt
     3841 tgccagaaac tggagattaa tgttccggtt tacgcgggga tgccgcaacc cattatgcgt
     3901 caacaaatcg ttgccgataa tattcacggt gaaaccggac tggatggccc ggtattcgag
     3961 ccgctaaccc gccaggcaga aagtacccac gcggtgaaat acatcattga taccctgatg
     4021 gcaagcgatg gcgatatcac tctggtgccg gttggtccgc tttcaaatat cgcggtggca
     4081 atgcgtatgc aacccgcgat cctgcccaaa atccgtgaaa ttgtgctaat gggcggcgct
     4141 tacggtacag gcaacttcac gccatctgcc gagttcaaca tctttgctga cccggaagca
     4201 gcccgcgtgg tgttcacctc tggcgttcca ttagtgatga tgggcctcga tctcaccaac
     4261 cagaccgttt gcaccccgga cgtgattgct cggatggaaa gggcaggcgg ccctgccgga
     4321 gagctgttca gcgacatcat gaacttcact ctcaaaacgc agttcgaaaa ctacggcctg
     4381 gctggcggcc cggtgcacga cgccacctgc atcggttatc tgattaaccc tgatggcatt
     4441 aaaacccagg agatgtacgt cgaagtggac gtcaacagtg gcccttgcta tgggcgcacc
     4501 gtctgcgacg agctgggcgt tcttggcaag cccgccaata ccaaagtcgg catcactatt
     4561 gataccgact ggttctgggg cttagtcgaa gagtgcgtgc gcggctacat caaaacccat
     4621 taagcgttga taacaagccg ggccgatagc gcaagatgca ggcccggcta tttctgatgg
     4681 gcagaaagcc gtcgcccaat atgacatgaa gagataatca ctatggatgt catgagaagt
     4741 gttctgggaa tggtggtatt gctggcaatt gcatttttgc tgtcagtaaa caagaagaag
     4801 atcagcctgc gtaccgttgg cgcggcgtta gtgttacagg ttgtgattgg cggcattatg
     4861 ctttggttac cgccagggcg ttgggtcgct gaaaaagtcg cttttggcgt gcataaagtg
     4921 atggcgtaca gcgacgcggg tagcgcattt atcttcggtt cgctggtcgg gccgaaaatg
     4981 gacacgctgt ttgatggcgc aggatttatc tttggtttca gagtattacc ggcaattatc
     5041 ttcgttacag cactggtgag tattctctac tacatcggtg tgatggggat tttaattcgc
     5101 attctcggcg gtatcttcca gaaagcgtta aatatcagca agatcgagtc attcgtcgcg
     5161 gtcactacca ttttcctcgg gcaaaacgaa attccggcaa tcgtcaaacc ctttatcgat
     5221 cgtctgaatc gcaatgaatt atttacagcg atttgtagtg gcatggcctc gattgctggt
     5281 tcgacaatga ttggttacgc cgccctgggc gtacctgtgg aatatttgct ggcggcatcg
     5341 ttaatggcga tcccaggcgg gatcttgttt gcccgcctgt taagcccggc tacggaatct
     5401 tcgcaggttt cctttaataa cctctctttc accgaaacac cgccaaaaag cattattgaa
     5461 gccgctgcga caggggcaat gaccgggctg aaaatcgccg ccggtgtagc gacagttgtt
     5521 atggcatttg tcgccatcat tgcgttaatt aatggtatta tcggcggcgt tggcggctgg
     5581 tttggttttg cacatgcctc gctggagtcc attttaggtt acctgttggc cccattggcg
     5641 tgggtgatgg gggttgactg gagtgatgca aatcttgccg ggagtttgat tgggcagaag
     5701 ctggcgatca atgaatttgt cgcttatctc aatttctcgc catatctgca aacgggtggc
     5761 actctggatg ctaaaaccgt ggcgattatt tctttcgcgt tgtgcggttt cgctaacttt
     5821 ggttctatcg gggtggtggt gggggcgttt tctgcggttg cgccacaccg tgcgccggaa
     5881 atcgcccaac ttggtttacg cgcgctggcg gcggcgacac tttctaacct gatgagtgct
     5941 actattgcag gattctttat tggtttagcg tagtgcgcaa tttatgagcc ggataagatg
     6001 cggtcaagcg tcgaatccgg caacattgtt acaccattgg cactaatgaa agcgcgttat
     6061 cggcagacag ggtagggttg tttaagctgc cgctggcgcg cgaaattgcc gcgcaggcta
     6121 ccgcaaaacg ggcgctgtcg cggaaactgt atccttcaag aaagctatat accaggcccg
     6181 ccataaaacc atcgtcagca ccaaaactgt ctactgtcgt atgcgctggc gcggtcagca
     6241 aaaattgttc tccatcctta tcgctgcaaa atacagattc atctttcaga taaacaaaaa
     6301 tgcgtttaac accttgttga tgcagcgaat ttacagctct gatgcgatcg ttgtcgtctt
     6361 taatcggttg cccccacaaa atttccagtt cattttgtgt tggtttcaga gtgtggatcc
     6421 ggctgtacca gttttttact ttgttcgctt taaattcgga aacggtatcg acaaacatcg
     6481 gaatttcatc agcaatggta aagacccact ccagggcttc aggtgtgaga ttacaatcag
     6541 ccagcaccac accagcatgg cgaatcaagt cccgtgaagt attgagcagt tgaggcgtta
     6601 gctgttgcag aatatgtgtg tcattgattg ccaggatagt ttcttcctgc ttattagcga
     6661 tagcgagata agttgcagtg ctgtgtccat gcagacgaat gcagtttgaa acgttcacgc
     6721 ccgcgcggcg cgtttcttcc agtaatgtct cgccgtaaaa atcgttgcca atagctgata
     6781 ttaaatgcac atcccgacca agcagagcca ggttatgcgc gatattacgt ccgacgccgc
     6841 ctgcggaaca gtgaacagaa cctggatgag aggctgcttg cgggtaacgg atatccgcca
     6901 ttccgcgaat atccatattg attgcaccca caaccacaca gtaatcctgc tcggtaagga
     6961 tatatccttt gcctttaatc aaacccttgc gcatcaaatc cataatatga gcggcaacac
     7021 gtgagcggct gatttgcaga atatcggcga tttcattctg ttgaatcagc gggttacgcc
     7081 gtaaaatttt aagaatctgc ttttccctgt cgttcatcga tcaggccacc gctttttcag
     7141 tttgttgcgc tttcagccag gcgatctctt ctgcccagat atccgggttg atggtttcaa
     7201 ggatcagcgg aatgccgtcg aaacggtcgt cctgcatgat ccagcggaac gcatcatgac
     7261 cgatattgcc ttcaccgagg ctatgatggc ggtcaacgcg gctgccaaag gtgcttttcg
     7321 catcgttaag gtgcatcccg cgcagatact taaagccaac aatacgggca aaatcagcga
     7381 acgttttttc gcattcggct ggagtacgca aatcataccc ggcagcgaaa gcatggcagg
     7441 tatcaatgca gacgccgaca cgggatttat cttccacgcc gtcgataatc gccgcgagat
     7501 gttcgaattt aaaccctaag ttactgccct gaccggcggt gttttcaatc accgctgtta
     7561 caccttgagt tttatccagc gcaatgttga tggattcggc aatacgcgca aggcaatcct
     7621 cttctgaaat ctgcatcaga tggctgccag ggtggaagtt gagcaaagaa agccccagtt
     7681 gttcgcaacg ctgcatttca tctataaagg catcgcgcga tttttccaga gcttcagtga
     7741 ccggatgtcc gaggttaatc agatagctgt cgtggggaag aatttgcgcc gatgtgtagt
     7801 gatatttttc acaggccgct ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac
     7861 gccactgacg ttggtttttg gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc
     7921 gaattgcggc atttgccaga ccgccagcag cactaacgtg cgctccaatg tatttcatgc
     7981 gaggactcct gttaaacccg ctggaggaaa acggtaatga tagcgggtta acaggagcaa
     8041 gatgtagtgg tttatgcgat gacgctttga atcacatagt taatcgcacc accaccaaca
     8101 atcagccagg caaacagtac cagtgccatc agcagaggtt tcgccccggc ttttttcagc
     8161 gcgctgacgt gagtggtcag acccagcgcc gccatcgcca ttgccagcag gaaggtatcc
     8221 agcgttacca gcatgttcac cacgctctgc ggtaacaggt ggaacgagtt aaagatggca
     8281 actacgatga acaagatggc aaaccacgga atagtgattt tgcttttctc gccgctgttc
     8341 gccccagaca gctgtttaac acgcgccgcc agcaggatga ggaacggagc cagcatcatc
     8401 acgcgcagca ttttggaaat aactgctgcg ttttccgcat ccgggctgat ggcatgacct
     8461 gccgccacca cctgcgccac ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc
     8521 ggactaaacc attgagacat cagcggatat atcgccgggt agaggaaaat agcgacggtc
     8581 ccgaagataa caacggttgc aacagccacg gttactttac tggcttccgc tttcactacc
     8641 ggctcagtcg ccagtaccgc ggcagcacca cagatactgc taccggcacc gatcaaccag
     8701 ctggtgtgct tatccagacc aaacactttc tgccccagga agcaagccag caggaaggta
     8761 ctggacagcg tcaacacgtc aatgatgatc ccactgatac cgacatcggc aatttgcgag
     8821 aacgtcagac ggaagccata aagaatgata cccagacgta ataaatattg cttggcaaac
     8881 agcacaccac cgtcacagct tttccagatg tgcggataga tggtgttgcc taaaaccatc
     8941 cccaacaaga ttgcgagggt gagggcacta aacccggcac ccgcaaccgc gggaatggaa
     9001 ccaccccaca gggcgacccc ggtgataact gcactcaggg ctaaccccgg aataaaatgc
     9061 cacagtgtac gatgttgttt ctgtaaggtg atattcgtca taaccctctc ctttaaccgg
//
LOCUS       DAZFJF010000161         9109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFJF010000161 DAZFJF010000000
VERSION     DAZFJF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.162"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1346
                     /gene="paoC"
                     /locus_tag="ACHKAU_004360"
     CDS             <1..1346
                     /gene="paoC"
                     /locus_tag="ACHKAU_004360"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO5533301.1"
                     /translation="LQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAG
                     ANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQV
                     DPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLE
                     KSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVS
                     AGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEAT
                     AGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNP
                     KTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDD
                     TDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1356..2312
                     /gene="paoD"
                     /locus_tag="ACHKAU_004361"
     CDS             1356..2312
                     /gene="paoD"
                     /locus_tag="ACHKAU_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO5533302.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            2363..2701
                     /locus_tag="ACHKAU_004362"
                     /pseudo
     CDS             2363..2701
                     /locus_tag="ACHKAU_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            2989..4266
                     /locus_tag="ACHKAU_004363"
     CDS             2989..4266
                     /locus_tag="ACHKAU_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5533303.1"
                     /translation="MRYKPNQYLICDSYGNYYLRITLPVYMQPFFEGKRTFVRSLHTS
                     NLRVARRKRDQIADEYHCLRESVAPVNSTIENTLELLRSKAKYAKTATRMQDTASSCP
                     SLLKILEIYLTINSTKKKPATLAKARKAVEMFLSYRKKPDIALQDVSRTTVTGWIEHM
                     QKTLSQQSIANYISPMAQLWELASSRYHDAPERALSPWRGHRLDVAQSRESYEAFSNK
                     ELLQVLQVFSGNSAENKEMMALCLIGAYTGMRINEIASLTIDDVKEIEGVLCFEITQG
                     KTKAAARVVPVHSLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHTQRFTRAKR
                     KALGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRTYSKNAASP
                     VELKKYIELLRYPEIEKGLSINKKSNLRRKTTP"
     gene            complement(4347..5060)
                     /locus_tag="ACHKAU_004364"
     CDS             complement(4347..5060)
                     /locus_tag="ACHKAU_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFO5533304.1"
                     /translation="MATAGILTIRAANTPEQHVQAVYKAEQDINSTKNENSKANTTKG
                     ENGFAIVTSGLASSGNDVYENYMALAFDSVDDVNVRRSADVTSYPVENGATVSDHVQI
                     KNNKFSLKGRITETPIKSDPGLLKSAGVNGNRRSLAIDYLNQIMDSRQPFLLVTENKT
                     FENVVLTGIEYTEEASESLVFDLSFEQIRLVSYGTVNTVALKTQPSKNIGANMKKRVN
                     TEKSSSEGEDTITPAFKQE"
     gene            complement(5095..6924)
                     /locus_tag="ACHKAU_004365"
     CDS             complement(5095..6924)
                     /locus_tag="ACHKAU_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533305.1"
                     /translation="MLEINTSKIKNTVTFSVDKDSLKKAKDSITGLKEFAENIKPAKL
                     RFDNVTKGYKKAQNEVDKIAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELK
                     LLDVSSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKIN
                     GNRKTQLAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETQGELI
                     QRAKLGGVDVNQMYNITEWAYRNGVDSMMGDQGARKYLDQMKDVRERAAKSYSEAEFL
                     KDKKGKSEWKGGDNAINELLNIGVINKSDLKKFADNPAGLISKAVNGMMKKGFSDSQI
                     GQRLEDLGDDLMLTSKYWQRSVKDVQESINQQKASGKWLTETQQESIVKFRELNRQLS
                     QLSDARQVAFVDGFMKSLDPKVTEEFMKNLSNLTPYFTKLGEAVGSLFEAIMKIVNWF
                     NRNDDKTDAIQKNLGDAPPLSNEGMKQHLSNLVPDQYKGAGTATTAPDNSHSLFNTIK
                     GLLFDDNASSVSDVKMSVSEAPITNLKQGALNNLAMTTPAYNLSPVFNINPTFEVVTE
                     VPLTINSDTSRLSDYVDFTARASRDSFLKSLTLTSLSGQSNGG"
     gene            complement(6939..7142)
                     /locus_tag="ACHKAU_004366"
     CDS             complement(6939..7142)
                     /locus_tag="ACHKAU_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533306.1"
                     /translation="MEVILKSKNGVHVHLDANDSRGLLNLKYLCSLLDVPYEGVKARM
                     FRLNESIDQALHHFLSKEGDKND"
     gene            complement(7227..8255)
                     /locus_tag="ACHKAU_004367"
     CDS             complement(7227..8255)
                     /locus_tag="ACHKAU_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5533307.1"
                     /translation="MILGNDYVDLIPLFQTHSTRNFLLSTLDFTDSVGVTSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTD
                     FQETLTDIYAEYIAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVSAM
                     KATVNASSTDTTKIFKEFDQIATDIIEKAQSQASAVERIVVFCKPEAFSAIRFSAGMA
                     NAFQYVSPLEEGNVVYQRRDLLPGVTAFTIPGTNIDVVKLVDPLHLAHMTSDAVAIPK
                     FAKGSNVYQNIYGAASSTFELINAAPAEVYSYSYESSRGDAVNVVTENSQMVVNHGVG
                     FSVQITVK"
     gene            complement(8274..8636)
                     /locus_tag="ACHKAU_004368"
     CDS             complement(8274..8636)
                     /locus_tag="ACHKAU_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533308.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVESKVFASLPADVECG
                     DLITSTGAKYVSGDDIYVVMSEFVTAGENKPVDVLRSNAGLVCIKADALNAVSEAAKT
                     ALIKKGFQLEGFHSVFTS"
     gene            complement(8633..8845)
                     /locus_tag="ACHKAU_004369"
     CDS             complement(8633..8845)
                     /locus_tag="ACHKAU_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533309.1"
                     /translation="MLFLNDKEQIIKYKDEMLKINPQITEMVAKYAGCSVEEVERCVE
                     KYFSPSSPSTPSLNELIKLKAKEITK"
BASE COUNT         2412 a         2091 c         2063 g         2543 t
ORIGIN      
        1 cccttcagca cttgcgtatc ggtgccgacc agagcgggaa aatcaccgct atctcacatg
       61 aaagctggtc tggaaacctg cccggcggca cgccggaaac ggcggtacag caaagcgaat
      121 tactctacgc cggggcgaat cgtcataccg gcctgcggct cgccacgctt gatttgccgg
      181 aagggaacgc catgcgtgcg cccggcgaag cccccggtct gatggcgctc gaaatcgcga
      241 tcgacgaact ggcggaaaaa gcgggcatcg atcccgtcga gtttcgcatc ctgaatgaca
      301 ctcaggttga ccccgccgac ccgacgcgct gcttctctcg ccgtcagctt atcgagtgct
      361 tgcgcaccgg agcggataaa tttggctgga agcagcgcaa cgccacaccc ggacaggtgc
      421 gcgacgggga gtggctagtc ggccacggtg ttgcggcggg ctttcgcaat aatctgctgg
      481 aaaaatcggg tgctcgggtt cacctcgaac aaaacggcac cgttaccgta gaaacggaca
      541 tgaccgacat tggcaccggc agctacacca ttctggccca gacggcagcg gaaatgcttg
      601 gcgtaccgct ggagcaggtt gcggttcacc tcggcgattc cagtttcccg gtttctgcgg
      661 gttctggtgg acaatggggc gcgaatacct ccacctccgg cgtttacgcc gcctgtatga
      721 agcttcgcga aatgattgcc tcggcagtcg ggtttgatcc tgagcagtcg cagtttgccg
      781 acggcaagat taccaacggt acccgaagcg ccacgctaca tgaagccacc gcaggcggca
      841 gactgacagc ggaagagagc attgaattcg gaacactgag caaagagtac cagcagtcga
      901 cctttgccgg gcattttgtg gaggtcggcg tgcatagcgc gacgggagaa gttcgggtcc
      961 ggcgtatgct cgctgtgtgt gctgcaggac gcatcctgaa tccgaaaact gcgcgcagcc
     1021 aggtcattgg cgcaatgact atgggcatgg gcgcggcact gatggaggag ctggcggtgg
     1081 atgaccgttt gggctacttc gttaatcacg atatggcggg gtatgaggtg ccggtccatg
     1141 cggatatccc aaaacaggag gtgattttcc tggatgatac cgaccccata tcctccccga
     1201 tgaaggccaa aggtgtcggt gagctgggcc tgtgcggcgt gagcgcggct atcgccaacg
     1261 cggtgtataa cgccaccggt attcgggtac gggattatcc catcactctg gataagctgc
     1321 tcgataagct gccggatgtg gtttaaggag aaacaatgtc atacccgctt tttgataaag
     1381 acgaacactg gcataagcca gagcaggcgt ttctcaccga tgaccaccgg accattctgc
     1441 gcttcgccgt agaggcgcta atgtccggta aaggagcggt gctggtgacg ctggtggaga
     1501 tacacggcgg cgcggcgcgc ccgctcgggg cgcagatggt ggttcgcgaa gatggtcgtt
     1561 actgcggttt tgtctctggc ggctgcgtgg aggccgctgc cgcttttgaa gcgctggaga
     1621 tgatgggctc aggccgcgat cgcgaaattc gctatggcga aggttcgccg tggtttgaca
     1681 tcgttctgcc ctgcggcggt gggatcacgc tgacgctcca taaactacgc tcggcacagc
     1741 ctctgctcgc cgtgctgaac cgtctggaac agagaaaacc ggcggggctg cgctacgatc
     1801 cgcaagcaca atcgttggtg tgcctgccca cgcaaacccg aacgggctgg aatctcaatg
     1861 gctttgaggt ggggttcagg ccatgcgtca ggctgatgat ttacggacgt tctcttgagg
     1921 cgcaggcaac cgcgagtctt gcagcagcca caggctatga cagccatatc ttcgatcttt
     1981 ttccggcctc agccagcgct cagatcgata ccgacacggc ggtcattttg ctgtgccatg
     2041 atctcaaccg ggagctgcca gcattgcagg ccgcgcgaga agcaaaaccc ttttatctcg
     2101 gcgcattggg cagccatcga acccacacgt tacgtctgca aaagctccac gagctgggat
     2161 ggtccaggga ggagacagcg caaatccggg cccccgtcgg gatattcccc aaagcccggg
     2221 atgcgcatac tctggcactc tccgtgctgg cagaaatcgc ctctgtacgt ctccatcatg
     2281 aggaggattc atgcctgccc ccgtcgtcct gatccttgcg gccgggcgtg gagagcgctt
     2341 tctcgcctcc gggggaaata cccataagtg tatcggctgg cgtcagtccc cggaggttgc
     2401 gccttatcgc tggccatttg aagaaaacgg gagaactttc gaccttgcga ttgaaccgca
     2461 gattacgact aatgatctgc gtctgatggt gaggctggct cttgccggcg gaggaataac
     2521 aattgccact caggaaactt tcaggccata tattgaaagc ggtaagcttg tatcgctgct
     2581 tgatgacttt cttccacaat ttccgggctt ctatctgtat ttcccacagc gtcgcaatat
     2641 tgcaccaaag ctccgcgccc tgattgacta cgtcaaagaa tggcggcagc aattggttta
     2701 aatagctgca cctgcactgc ctgatgtcag aacagtattt tgatgaattg ccaaggttac
     2761 aatggcacaa atgcggcaca ggaggaaacg tggtgtattt aaatatgggg taactctttg
     2821 attttaatgg tgccgataat aggagtaaaa cactcgatta atctcgtgtt aatgccatca
     2881 cctacgacca ttactaattg attgaatctt atagaaaaat catcattttc tatgcgtcac
     2941 aatcaatttt cacattacaa acgctccgac actttagccg gagattttat gagatacaag
     3001 cctaaccaat acttaatctg tgattcttac ggtaactact atttgaggat cacgttgcct
     3061 gtgtatatgc agcccttttt tgaaggaaaa aggacgtttg tcaggagtct acatacaagt
     3121 aatcttcgtg ttgcacgtag aaagcgtgat cagattgcag atgaatacca ttgtctacgg
     3181 gagagtgtcg cccctgtaaa cagcacaata gagaacacgc tggagctgtt acgcagtaag
     3241 gctaaatacg ccaaaacagc taccagaatg caagatacag cgtcttcgtg tccgtcattg
     3301 cttaaaattc ttgaaatcta cctgacaatt aacagcacga agaagaagcc agccacttta
     3361 gctaaggcaa gaaaagcggt agagatgttt ctctcctacc gtaaaaagcc tgatattgca
     3421 ttgcaagatg tgagccgcac cactgttaca ggctggattg aacacatgca aaaaaccctt
     3481 tcacaacaat caattgcaaa ttatatcagc ccaatggccc agctatggga gttagcttca
     3541 tcacgttacc acgatgcgcc agaaagggcg ctctccccct ggcgagggca taggcttgat
     3601 gtggcacaaa gtagagagag ctatgaggca ttttctaaca aagagctatt gcaggtgttg
     3661 caagtatttt ccggtaattc agcagaaaac aaagaaatga tggctttgtg tcttatcggt
     3721 gcttatacag gtatgcggat caatgagata gcaagtctca caatagacga tgtgaaagag
     3781 atcgaaggtg tgctgtgttt tgaaatcaca cagggaaaaa cgaaagctgc ggcgcgtgtt
     3841 gttcctgtac atagccttat cactccattg gtgttatctc ttcgtgaaaa gcctcacaat
     3901 ggctttttgt tctatcacgc cagcattaca gagcgtgctg acggtaaacg ctctacgtgg
     3961 catacacaac gatttacaag agctaaacga aaggctttag gggaaaaggg aacagaaagg
     4021 aaggtgtttc attctctgag acacggagta gcacagcttc ttgatcgaaa tcaaattcca
     4081 gaagacagga tcgcccttct cctgggccat acacgcggca atacagagac attccgcaca
     4141 tatagcaaga atgcagcttc tccagtagag cttaaaaaat atattgagct tctacgctac
     4201 cctgaaatag agaaaggctt atcaatcaat aaaaaatcaa atttaaggcg taaaacaacg
     4261 ccatagacgc aataaagagg cttcaacata attggtagcc tcttttataa ttacatcgtt
     4321 acagagcgtt ttagaggcat ttacggctat tcctgtttaa aggcaggagt aatagtgtct
     4381 tcaccttcac tacttgattt ttcagtgtta acacgttttt tcatattagc cccaatgttc
     4441 ttagatggct gtgttttgag agctacagtg tttacagtgc cataagaaac taatctgatt
     4501 tgttcaaagc taagatcaaa taccagagat tcgcttgcct cttctgtgta ttcgatgcct
     4561 gttaaaacaa cgttctcaaa agttttattt tctgtaacaa gaagaaaagg ctgtctactg
     4621 tccataatct gattcaggta atcaatagct aatgatcttc tgttcccgtt cacccctgca
     4681 ctctttaaca agccaggatc gcttttaatc ggtgtttcag tgatccttcc tttaagagaa
     4741 aacttattgt ttttaatttg aacgtgatca gacacagtag caccattttc tacggggtag
     4801 cttgtaacat ctgccgaacg tctcacgttc acatcgtcaa cactatcaaa ggccagtgcc
     4861 atataatttt cataaacgtc attgccagaa gatgccaagc cactggtaac aatagcaaat
     4921 ccattttcgc cttttgttgt attagctttg ctattttcgt tttttgtaga gtttatatct
     4981 tgttctgctt tgtaaacagc ctgaacgtgt tgttctggag tatttgctgc acgtatggtt
     5041 aaaatcccgg cggtcgccat aataataatt ttccttgtaa tgttatggcg ggaattaccc
     5101 gccgttagat tgtcctgata agctggttaa tgttaatgat ttcaagaagc tatcccgact
     5161 tgctctcgct gtaaaatcca cataatcgct taatcttgat gtgtcagaat taatagtaag
     5221 aggaacctcc gttaccactt caaaggttgg atttatatta aacacaggag ataaattata
     5281 agctggagtt gtcattgcaa gattatttaa agcaccctgt ttgagattgg ttatcggtgc
     5341 ttcgctgact gacattttta catctgacac agaagaagca ttatcatcga aaagcaatcc
     5401 cttaatcgtg ttaaagagag aatgactatt atcaggtgca gttgttgcag ttccagcgcc
     5461 tttatattgg tcaggaacga gattagaaag atgctgtttc atcccttcat tgcttaatgg
     5521 cggtgcatca cctaaatttt tctgaatagc atctgtctta tcatcattac gattaaacca
     5581 gtttacaatc ttcataatag cctcaaagag acttcctaca gcttctccca gcttcgtaaa
     5641 atacggagtg agattagaaa ggtttttcat aaactcttcc gttacttttg gatcgagaga
     5701 tttcataaag ccgtctacga atgctacttg tcttgcatca gaaagctgtg aaagctgtct
     5761 gtttagttct ctgaatttaa cgatagattc ttgctgtgtt tctgttagcc attttccaga
     5821 ggctttttgc tgattaatac tttcttgtac atctttaacg gaacgttgcc aatattttga
     5881 cgtaagcatc aaatcatcgc ccaggtcttc taaacgttgt ccaatttggg agtctgaaaa
     5941 cccttttttc atcattccat taacagcctt agaaattaat ccggctggat tatcagcaaa
     6001 ttttttaagg tcgcttttgt taataacacc gatgtttaga agttcattaa tagcattatc
     6061 accgcctttc cattcagatt ttcctttctt gtctttcaag aattcagctt cactgtagga
     6121 ttttgcggca cgttccctaa catctttcat ctgatccagg tatttcctag ccccttgatc
     6181 acccatcata gagtcaacgc cgtttctata ggcccattct gtaatgttgt acatctggtt
     6241 aacgtctaca ccgcccagct tggcccgttg aattagctcc ccttgcgtct ctgcgttagc
     6301 gaagctgtta gccataaatt cagaggtttt actaacagcc atcacgccag cggcagccgt
     6361 agcgcctcca aataacagag cgcccatcgt ggcggaattc cctgatcctc ccttaacggg
     6421 ggctaattga gtttttctat tgccattaat cttacgttgt tgttgttgta agcgtttcat
     6481 ttggctattc atacgtgcaa gcgagatagc gccttgttcg tattgtttcg ctatctctct
     6541 agcctgtgca atagctttgt attgttccgc tacagagagg cggtgcattg cacttataga
     6601 gcttgacaca tcaaggagtt ttaattctgc tttctcctta cgtgctgcta tttgttgctg
     6661 ttgccttgcc tgtgctcttg ctgcggcagc ttgtgctctt tgattctgtt tttcggtttt
     6721 agctttctgt tgagcaatct tgtctacttc gttttgtgct ttcttatagc ctttagtgac
     6781 attatcaaat cttaatttag ctggcttgat gttttctgcg aattccttta agcctgtaat
     6841 agagtctttc gcttttttca agctgtcttt atctacacta aatgttactg tgttcttaat
     6901 tttagaagtg ttaatttcta acatttaaaa tccccttatt aatcattttt atcaccttct
     6961 ttacttaaga aatggtgtag agcctgatca atactctcgt ttaatctgaa catacgggct
     7021 tttactcctt catatggaac atctaggaga gaacagagat atttcagatt taacaagcct
     7081 ctagaatcat tagcatctaa atgtacgtgt acaccatttt ttgatttcaa aatcacttcc
     7141 atattttaat cacctctcaa aatttaaaaa aataaaaaaa aaggcgaagc gtgaatacac
     7201 gccacgcctc tattacacgt taaatattat ttaacagtga tttgaacgga gaagcctaca
     7261 ccgtggttta caaccatctg gctattctct gtaacaacgt taacagcatc accacgagaa
     7321 gattcatagc tatagctata cacctcagca ggagcggcat tgatcagttc aaaagtgctg
     7381 gaagcagccc cgtagatgtt ctgatataca ttagagcctt tagcaaactt cggaatcgca
     7441 accgcatcag aagtcatatg tgcaaggtgt agcggatcta ccagtttaac aacatcaatg
     7501 ttagttcctg ggatagtgaa cgctgtcacc cctggcagca ggtcacgacg ctgatacaca
     7561 acgttcccct cttccagtgg gcttacatac tggaatgcgt ttgccatacc agcagagaat
     7621 cgaattgcgg agaaggcttc aggtttacag aaaacaacaa tacgctctac agcagacgcc
     7681 tggctttgtg ctttctcgat aatgtcagta gcaatctgat caaattcttt aaaaatcttc
     7741 gtggtgtcag tgctggaagc gttcacagtg gctttcatag ctgatacgtt aaacagcttg
     7801 ccccattcga taagaacatc atcagttttc ggtgtataag ttttgcccga gaacaaagaa
     7861 gcagccaata cgctctcttt tgtacgctga tacgctacgt ggtgtttagc gatatattcc
     7921 gcataaatat cagtgagtgt ttcctggaaa tcagtgccag gtttacgttt gccttgaaca
     7981 tcagaaggac ggatcacatc ttcacgcagg aagtaaggga tctcgatcag ccattcttta
     8041 ccttgttctc gcttagtaac attgtgttca gaactaaaac ggcttgtctc tttgttaaag
     8101 agagattcgt tgctttcgac aagctgggaa acagctactt tatgagatgt aacccctaca
     8161 gaatccgtaa aatcaagagt agagagcagg aagttacgcg tgctgtgtgt ctggaacaga
     8221 gggatcaaat caacgtaatc attgcctaga atcatttatt attattcctt ttattaagaa
     8281 gtgaaaacag aatggaaccc ttcaagctgg aagccttttt taattaatgc tgtttttgct
     8341 gcttcactta cagcattcag cgcatcagct ttgatgcata caagcccagc attgctacgt
     8401 aaaacatcta caggtttgtt ttctccagcc gttacaaatt cactcataac aacatagata
     8461 tcatctcctg atacatattt agcgccagtg ctggtgatca agtcgccgca ttcaacatca
     8521 gcaggaagag aagcaaaaac cttgctttct acagaggcaa acggatcagt acaataactt
     8581 acaatatctg tgaaagtctt tcgaactgga aacagagtgt ctaaatcaat aatcatttag
     8641 taatctcctt agcttttaat ttgattaatt cattaaggga aggtgtagac ggagaagaag
     8701 gagaaaaata tttttcaaca catctttcta cttcttctac agaacaccca gcgtatttag
     8761 ctaccatttc ggtaatttgt ggatttattt tcagcatttc atctttatac ttgatgattt
     8821 gctctttatc atttaagaac aacatttata taaaccttgt taccttgttt aaagtaagaa
     8881 atacaacttt taagttgcat aacgggcaaa ataaaaggca gcatttagag ccgcctttta
     8941 ttaacttgta cctatgtaat taccctatgt tatgcatact ccaagatgta tgcataacaa
     9001 aggaaaaaag aggtaataat gacacagtat ttacttaagg aggagaagcg gggagcggag
     9061 attttactcc cctttaacga caagaagaat ttttaatatc gaaccaata
//
LOCUS       DAZFJF010000162         9075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFJF010000162 DAZFJF010000000
VERSION     DAZFJF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.163"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2798
                     /gene="eae"
                     /locus_tag="ACHKAU_004370"
     CDS             <1..2798
                     /gene="eae"
                     /locus_tag="ACHKAU_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HFO5533310.1"
                     /translation="RTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENYFKLSSDSK
                     LLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMMKAGPGQQI
                     ILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDDKALNYAAQ
                     QAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQSGNNFDGS
                     SLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNVFIDQDFSG
                     DNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPSYP
                     ALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNEND
                     LLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSLNI
                     PHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQDYQAILPA
                     YVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTADKTSAKADG
                     IEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTLKSATPGQV
                     VVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTVRVMKEGAP
                     VVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVGTEVKATTV
                     EFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSSNTKIASVD
                     NSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDAENKCKTNS
                     ANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVSSTYDLVTKNQ
                     LINVGVNNKNAFSVCVK"
     gene            complement(3013..4233)
                     /gene="escD"
                     /locus_tag="ACHKAU_004371"
     CDS             complement(3013..4233)
                     /gene="escD"
                     /locus_tag="ACHKAU_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO5533311.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
     gene            4376..5431
                     /gene="sepL"
                     /locus_tag="ACHKAU_004372"
     CDS             4376..5431
                     /gene="sepL"
                     /locus_tag="ACHKAU_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO5533312.1"
                     /translation="MANGIEFNQNTASVFNSNSLDFELESQQLTQKNSSNSYSPLLNL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKE
                     LSLKNSGLLEQHDPNLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLDLLGE
                     DDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSEKGASLLDWFMEIKDLPEREKH
                     LKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYTDIIAISINKDKDVI
                     LNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQRD
                     NTLLMIGKVIDYKEDVI"
     gene            5489..6067
                     /gene="espA"
                     /locus_tag="ACHKAU_004373"
     CDS             5489..6067
                     /gene="espA"
                     /locus_tag="ACHKAU_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO5533313.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            6080..7222
                     /locus_tag="ACHKAU_004374"
     CDS             6080..7222
                     /locus_tag="ACHKAU_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFO5533314.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            7243..8187
                     /gene="espB"
                     /locus_tag="ACHKAU_004375"
     CDS             7243..8187
                     /gene="espB"
                     /locus_tag="ACHKAU_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO5533315.1"
                     /translation="MNTIDYTNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVS
                     KLMLEIQKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGA
                     ISSVLGILGSFAAINSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAV
                     EDASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAA
                     VDKITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQ
                     EVRRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            8194..8601
                     /gene="cesD2"
                     /locus_tag="ACHKAU_004376"
     CDS             8194..8601
                     /gene="cesD2"
                     /locus_tag="ACHKAU_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO5533316.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            8638..8859
                     /gene="escF"
                     /locus_tag="ACHKAU_004377"
     CDS             8638..8859
                     /gene="escF"
                     /locus_tag="ACHKAU_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO5533317.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            8865..>9075
                     /locus_tag="ACHKAU_004378"
     CDS             8865..>9075
                     /locus_tag="ACHKAU_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO5533318.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMD"
BASE COUNT         2717 a         1686 c         2040 g         2632 t
ORIGIN      
        1 cccggacccg gcacaagcat aagctaaaaa aaacatttat tatgcttagt gctggtttag
       61 gattgttttt ttatgttaac cagaattcat ttgcaaatgg tgaaaattat tttaaattga
      121 gttcagattc aaaactgtta actcaaaatg ccgctcagga tcgccttttt tatacgttaa
      181 aaacaggtga aactgttgcc aatatttcta aatcacaggg tatcagttta tcggtaattt
      241 ggtcactgaa taaacattta tacagttccg aaagcgaaat gatgaaggct ggacctggtc
      301 agcagatcat tttgccactc aaaaaactgt ctgttgaata tagtgcctta cctgtcttag
      361 gttcggcacc tgttgttgct gcaggtggtg tcgctggtca tacgaataaa atgactaaaa
      421 tgtccccgga cgcgactaaa agcaacacga ccgatgacaa ggctctaaat tatgcggcac
      481 aacaggccgc gagccttggt agccagctcc agtcgcgctc actgaacggc gattacgcga
      541 aagataccgc tcttggtatg gccagcagcc aggcttcgtc acagttgcag gcctggttac
      601 aacattatgg aacggcagag gttaatctgc agagtggtaa taactttgac ggtagttcac
      661 tggacttctt attaccgttc tatgattccg aaaacatgct ggcatttggt caggtcggtg
      721 cgcgttacat tgactcccgc tttacggcaa atttaggtgc tggccagcgt tttttccttc
      781 ctgaaaatat gttgggctat aacgtcttca ttgatcagga tttttctggt gataataccc
      841 gtttaggtat tggtggcgaa tactggcgag actatttcaa aagtagcgtt aacggctatt
      901 tccgcatgag cggctggcat gagtcataca ataagaaaga ctatgatgag cgcccggcaa
      961 atggttttga tatccgcttt aatggctatt taccatcata tccggcatta ggcgccaaac
     1021 tgatgtacga acagtattat ggtgataatg ttgctttgtt taattccgat aagttgcagt
     1081 cgaatcctgg cgcggcgacc gttggtgtaa actacactcc gattcctctg gtgacgatgg
     1141 ggatcgatta ccgtcatggt acgggtaatg aaaatgatct cctttactca atgcagttcc
     1201 gttatcagtt tgataaaccg tggtctcagc aaatcgagcc acagtatgtt aacgagttaa
     1261 gaacattatc gggcagccgt tacgatctgg ttcagcgtaa taacaatatt attctggagt
     1321 acaaaaagca ggatattctt tctctgaata ttccgcatga tattaatggt actgaacaca
     1381 gtacgcagaa gattcaattg atcgttaaga gcaaatacgg tctggatcgt atcgtctggg
     1441 atgatagcgc attacgcagt cagggcggtc agattcagca tggcggaagc caaagcgcac
     1501 aagactacca ggctattttg cctgcttatg tgcaaggcgg cagcaatatt tataaagtga
     1561 ccgctcgcgc ctatgaccga aatggtaata gttctaataa tgtacagctc actattaccg
     1621 ttttaccgaa tgggcaggtt gtggaccagg ttggggtaac ggactttacg gctgataaaa
     1681 catcggctaa agcggatggc atagaagcta ttacctatac cgcgacggtt aaaaagaatg
     1741 gtgtagctca ggctaatgtc cctgtaacat ttagtattgt atccgggact gcaactcttg
     1801 gggcaaatag tgccagaacg gatggtaacg gtaaggcgac cgtaacgctg aagtcggcta
     1861 cgccaggaca ggtcgtcgtg tctgctaaaa ccgcggagat gacttcgcca cttaatgcca
     1921 gcgcggttat atttgttgat caaaccaagg ccagtattac tgagattaag gctgataaaa
     1981 caacagcgaa ggcagatggt tctgatgcga ttacctatac tgtcagagtg atgaaggagg
     2041 gggcacccgt agtagatcag aaagtgacct tttctaagga ttttgggacc ctgaataaga
     2101 ctgaagcaac aaccgatcag aatggttatg ctactgtaaa attatcatcc aatactcctg
     2161 gcaaggccat tgttagtgca aaagtgagtg gagtaggtac agaagttaag gctactaccg
     2221 ttgagttttt tgccccgttg agtattgatg gtgataaagt gaccgtaatt ggtactggta
     2281 tcacgggggc tctgccaaag aactggttac agtatggtca ggttaagcta caggcaacag
     2341 ggggcaatgg aaaatacaca tggaaatcca gtaatactaa aattgcttct gttgataact
     2401 cgggagtgat aaccttaaat gaaaaaggga gtgccacaat tactgtagta tctggtgata
     2461 atcagagtgc gacatacaca attaatgcac cgggtagtat tgtaattgct gtggataaaa
     2521 atactcgagt tacgtatttt gatgccgaaa acaaatgtaa gacaaatagc gcaaatttag
     2581 cacagtcaaa agaactattg gccaatatct attcaacatg gggtgctgca aataaatatc
     2641 cttactattc tggttctaaa tcattgactg cttggattaa acaatcctct tctgaacagt
     2701 catcaggtgt atcaagcaca tatgatttgg ttacgaagaa ccagttgatc aatgttggag
     2761 taaacaataa gaatgctttt tctgtttgtg taaaataatt taattacatc tcaaatcccg
     2821 gaaaataaaa ttcattttcc gggatttttt attgtaccta cttgaatctt ttaataaacg
     2881 tttcatgtta aaatcaatgt ctcatttcgg aataagtttt tcgtgtatct tgtttgatag
     2941 agaccttgtt taccatatct acaacatctg cacaataaaa aaccctccga agagggggaa
     3001 gataataata agttaataag acaacggaat atgtatatat tcgtctgggc gggagatgtt
     3061 gataccatct ttaggatcaa tcgcgtctat gacatagcct cctttcagaa tgctaccaat
     3121 tcccaggctt gtaccgttat caagagcaat tgttggattc ttcatcgtgc cgctgattct
     3181 gagtattttt ccggggaaaa tatctgctga aatgtacggc tgaatgttct gaaatagtat
     3241 cttgacgtta ctatttttgt tcatcgcatt tataatggca aggatcttac tctcgttctg
     3301 ttgagaaacc tcccccgcaa caatgatgac atcattcttt ttgaaaatat tgacatagtt
     3361 tattaattta ttctttttga actctgatgc cagttcattg attttattgt tactgttgtt
     3421 atgtatttcc catccagcaa cccctggcgt attcagtaac agattttcaa cttcgctcca
     3481 ttttggtgaa gggggaatgt atcccgataa caggaagaat ccaggcttat taccttttgt
     3541 aatgatgata tccttataac cgtattccgt taaaatatca ttaatcgcgg agatgatttg
     3601 gttattgcaa atgatattgt tgtaataaaa aatatttttt tctttcaggt aattaaaaaa
     3661 tggagttaaa tcagtggttg attcgcaacg cccataaagc gcgacatttt tcccatgcca
     3721 taccagagtg attgcatgaa gtttgttttc ttttagctgc tggtatacat cagctctggt
     3781 aaacgctggg ggttcagctt ttggggaaag caaaacgtaa ctgccgatgg cgccagacaa
     3841 cgcaatcgca gcaccaatca cgctagccag gataagacgc tttttacgtc gatttttggc
     3901 agagaacctt tctggcacat cagacaatgg caaggtgtgg tcaatatcac caatgatgaa
     3961 gcaacatccc gctatatcga tgacctttcc gacaggtaat gactgatctg cttcataggg
     4021 ggtgggctgt ccatcaatcc aaaattcaac cggagataaa aggaaaacac cttcctcacg
     4081 tatatcaagt aaaaactgat tgaggccttg ttcaagggga aaataaacaa tatcgttatc
     4141 ttctgtgcct atcgttaggt ttcccatcgg aagttgtaat tcccgattac gcatcgcacc
     4201 attgagaagt tttattttat atgaggataa catttttaaa ttttattcat cctggtggtt
     4261 gaacaatgag aaaaaatatg gtgaacttac atcaactaag aatagagtag aaaggaagct
     4321 gttgtacagt actatttaat ggaatattca taattaatga ttacgtgagt ttccaatggc
     4381 taatggtatt gaatttaatc aaaacaccgc atctgttttt aattctaatt cattagattt
     4441 tgaattggaa tctcaacaat taacgcaaaa aaattcttct aatagttatt ctccattatt
     4501 aaatttacaa aatgaattag cgatgattac tagttcatcg ctttctgaaa cgattgaagg
     4561 gctaagcctg ggatatcgca aaggcagtgc aaggaaagaa gaggaaggta cgacaacgga
     4621 gaagctgctt aatgatatgc aagagctgct tactctgacc gatagcgata aattaaaaga
     4681 actatcatta aagaacagtg ggctgctgga acagcatgat cctaatttgg ctatgtttgg
     4741 taatatgcca aaaggggaga ttgttgcact tatatcttct ttattacaat ctaagtttgt
     4801 taagattgaa ttaaaaaaga aatacgccaa attattatta gatttattag gcgaagatga
     4861 ttgggagttg gctctgcttt cctggttagg ggtgggtgag ttaaatcagg aaggtatcca
     4921 gaagatcaaa aagctttatg aaaaggccaa ggatgaggat tctgaaaagg gagcctcttt
     4981 acttgactgg tttatggaga ttaaggatct tccagagcgt gagaagcact taaaggttat
     5041 tattagggcg ctatcgttcg atctgtctta tatgtcttct tttgaagata aggtgaaaac
     5101 atcttcaatt attagcgatt tatgccgggt aatcattttt ttatcgcttg ataactatac
     5161 agatattatt gcaatctcta ttaataaaga taaagatgtc attttaaatg aagtattgtc
     5221 tattattgaa catgtatggt taacagaaga ctggctcctg gaaagccctt ctcgggtatc
     5281 gattgtcgaa gataaacatg tttattattt ccacctattg aaagactttt ttgcatcatt
     5341 acctgacgct tgttttattg atagggaaca gagagataat acattattaa tgattggtaa
     5401 agtaattgat tataaggagg atgttatttg atattggttt tttaatcgtt tttggtcttg
     5461 ctaagaaaga ttattaagag gtatatacat ggatacatca actgcaacat cagttgctag
     5521 tgcgaacgcg agtacttcga catcgacagt ctatgactta ggcagtatgt cgaaagacga
     5581 agtagttcag ctatttaata aagtcggtgt ttttcaggct gcgcttctca tgtttgccta
     5641 tatgtatcag gcacaaagcg atctgtcgat tgcaaagttt gctgatatga atgaggcatc
     5701 taaggagtca accacagccc aaaaaatggc taatcttgtg gatgctaaaa ttgctgatgt
     5761 tcagagtagt tctgacaaga ataagaaagc caaacttcct caagaagtga ttgactatat
     5821 aaatgatcct cgcaatgaca ttacagtaag tggtattagc gatctaaatg ctgaattagg
     5881 cgctggtgat ttgcaaacgg tgaaggccgc tatttcggcc aaatcgaata acttgaccac
     5941 ggtagtgaat aatagccagc ttgaaataca gcaaatgtca aatacgttaa acctattaac
     6001 gagtgcacgt tctgatattc agtcactgca atacagaact atttcagcaa tatcccttgg
     6061 taaataaccg gagataacta tgcttaatgt aaatagcgat atccagtcta tgaggtctgg
     6121 ggccagcgct gctacggcta cttcaggtat taatcagcct gaagtgactt ccgcgctgga
     6181 tttacaactg gttaaatcca cggccccatc agcaagttgg acagaaagta ccgctttacc
     6241 aacgccaccg gcaggacact cattagtgac gccctctgtt gctgaggatg tcctgagtaa
     6301 actgtttggt ggtattagtg gtgaagttag gagtcgaaca gagggaaccg aaccacagcg
     6361 cagcactcag aatgcaagct ctggctatcc ttatctttct caggtgaata acgttgaccc
     6421 tcaggccatg atgatgatgg tcaccctgtt atccctggat gcttcagcgc agaaagttgc
     6481 gagtatgaaa aactccaacg agatctacac ggatgggcaa aataaggcgc tggataataa
     6541 aacgctggag tttaaaaagc agctcgaaga acaacagaaa gccgaagaga aagcacaaaa
     6601 aagtaaaatt cttggtcagg tctttggttg gttgggcgtc gcggtaactg ctattgcagc
     6661 tatttttaac ccggcactct gggctgttgt tgccgttagc gcaacagcaa tggcactgca
     6721 aactgcagtt gatgtaatgg gcgataaggc cccgcaggcg ttaaagacag cagcgcaagt
     6781 ctttggcgga gtatctattg ctgcaagtat tctgacagcc ggcattggcg gggtgtcttc
     6841 acttatgtct aaagttggcg atgtcgctaa caaagttggt tcaaacatcg tgaaaacagt
     6901 tactaaactg gccgatgtat ttgttgagaa cgttgtttca aaagttgcgg caacggcaaa
     6961 tggatttacg acctctgcac gttctattgg gacaactgtg ctgaataatg attctgcgca
     7021 atacagcata ttggctcagt tatctgctta cgcggtacaa aacttaactc gacagagtga
     7081 aaacctgggt gagagtgcga aggttgagct ggataaagct gcagccgagt tgcgaaacca
     7141 ggcaagctat ttacaaaatg cttctcagtt aatatccgac tcagcacgag taaatagccg
     7201 tattgttagt ggtcgagttt aattattaaa gagaatttaa ttatgaatac tattgattat
     7261 actaatcaag taatgacggt taattctgtt tcggagaata ctaccggctc taatgcaatt
     7321 accgcatctg ctattaattc atctttgctt accgatggta aggtcgatgt ttctaaactg
     7381 atgctggaaa ttcaaaaact cctgggcaag atggtgcgta tattgcagga ttaccaacag
     7441 caacagttgt cgcagagcta tcagatccaa ctggccgttt ttgagagcca gaataaagcc
     7501 attgatgaaa aaaaggccgc tgcaacagcc gctctggttg gtggggctat ttcatcagta
     7561 ttggggatct taggctcttt tgcagcaatt aacagtgcta cgaaaggcgc gagtgatatt
     7621 gctcaaaaaa ccgcctctac atcttctaag gctattgatg cggcttctga tactgcgact
     7681 aaaacgttga ctaaggcaac ggaaagcgtt gctgatgctg ttgaagatgc atccagcgtg
     7741 atgcagcaag cgatgactac agcaacgaga gcggccagcc gtacatccga cgttgctgat
     7801 gacattgccg attctgctca gagagcttct cagctggctg aaaacgctgc agatgccgct
     7861 cagaaggcaa gtcgggcaag ccgctttatg gctgcagtag ataagattac tggctctaca
     7921 ccatttattg ccgttaccag tcttgccgaa ggcacgaaga cattgccaac aacggtatct
     7981 gaatcagtca aatctaacca tgagattagc gaacagcgtt ataagtctgt ggagaacttc
     8041 cagcagggta atttggatct gtataagcaa gaagttcgca gagcgcagga tgatatcgct
     8101 agccgtctgc gtgatatgac aacagccgct cgcgatctca ctgatcttca gaatcgtatg
     8161 ggtcaatcgg ttcgcttagc tgggtaattg atcatggtcg atacgtttaa tgatgaagtg
     8221 cttaatcact atcttgaaca aaaagggtac acaatacaga aggagtttct ttgtggcagt
     8281 gcctttttta tcggatggcg gattgagacc tcttttttct cattagcgta cagacttgat
     8341 gaacaagaac tgattttgtg ctcttttgaa gcacgtaacc aaacagggct taacggccct
     8401 gttttatcac tgactcgctt gctcgaagag ttgtaccacc atttttcggg tattaagaaa
     8461 atcagtgcga tgaaatctaa gattggttca gattcagaac gtcaaaagcg cgaagagttg
     8521 tttaattact tcatcagaaa gggcgctgtg cagcaagaaa cagaagacgg aatttggttc
     8581 gtaatgaatg taaatagtta gaaattttta tttctattaa ctattgagga aaatttaatg
     8641 aatttatctg aaattactca acaaatgggt gaagtaggta aaacgctgag tgattctgtg
     8701 ccagagttac ttaatagcac cgatttggtt aatgaccctg aaaaaatgtt agagctgcag
     8761 ttcgcggttc agcaatattc tgcttatgtt aacgtagaaa gtggaatgct gaaaacgata
     8821 aaagatttgg tctcaaccat ttccaaccgt agcttttaat ttttatggtt aatgatgttt
     8881 ctgccaacaa gatactggtg tgggcggctg tagctgcggc aaatcataag cttcccaaat
     8941 atgcagaatc tatcctgaat gtattgccgc aaattatacc cgataaaaaa gatatcgcac
     9001 atttagaatt tattatttta tatggattaa atagaaaaaa tgatgcggta aaggctctgg
     9061 aggactttat ggatg
//
LOCUS       DAZFJF010000163         8708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFJF010000163 DAZFJF010000000
VERSION     DAZFJF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.164"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(344..607)
                     /gene="rpsT"
                     /locus_tag="ACHKAU_004379"
     CDS             complement(344..607)
                     /gene="rpsT"
                     /locus_tag="ACHKAU_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO5533319.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            710..928
                     /locus_tag="ACHKAU_004380"
     CDS             710..928
                     /locus_tag="ACHKAU_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO5533320.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            936..1877
                     /gene="ribF"
                     /locus_tag="ACHKAU_004381"
     CDS             936..1877
                     /gene="ribF"
                     /locus_tag="ACHKAU_004381"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO5533321.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1920..4736
                     /gene="ileS"
                     /locus_tag="ACHKAU_004382"
     CDS             1920..4736
                     /gene="ileS"
                     /locus_tag="ACHKAU_004382"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO5533322.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4736..5230
                     /gene="lspA"
                     /locus_tag="ACHKAU_004383"
     CDS             4736..5230
                     /gene="lspA"
                     /locus_tag="ACHKAU_004383"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO5533323.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5318..5767
                     /gene="fkpB"
                     /locus_tag="ACHKAU_004384"
     CDS             5318..5767
                     /gene="fkpB"
                     /locus_tag="ACHKAU_004384"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5533324.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5769..6719
                     /gene="ispH"
                     /locus_tag="ACHKAU_004385"
                     /gene_synonym="lytB"
     CDS             5769..6719
                     /gene="ispH"
                     /locus_tag="ACHKAU_004385"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO5533325.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6785..7699
                     /gene="rihC"
                     /locus_tag="ACHKAU_004386"
     CDS             6785..7699
                     /gene="rihC"
                     /locus_tag="ACHKAU_004386"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO5533326.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7866..8687
                     /gene="dapB"
                     /locus_tag="ACHKAU_004387"
     CDS             7866..8687
                     /gene="dapB"
                     /locus_tag="ACHKAU_004387"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO5533327.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1985 a         2157 c         2480 g         2086 t
ORIGIN      
        1 agatctaata aggagatatt tatgaatggt taaagtgtat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccacattct ttgtagggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga aagtgtaacg gcatgtctgc aaaagccagc atcttttgac
      241 tatctggcaa ccggtagtgt gttctctcac gcatcataaa agcagcaggc ataaaaaaac
      301 ccgcttgcgc gggctttttc acaaagcttc agcaaattgg cgattaagcc agtttgttga
      361 tctgtgcagt caggttagcc ttatgacgtg cagctttgtt tttgtggatc agacctttag
      421 cagcctgacg gtccacgatc ggttgcattt cgttaaatgc tttctgtgca gcagctttgt
      481 cgccagcttc gatagctgcg tatactttct tgatgaaagt acgcatcata gagcgacggc
      541 ttgcgttgtg cttacgagcc ttttcagact gaatggcgcg cttcttagct gatttgatat
      601 tagccaaggt ccaactccca aatgtgttct atatggacaa ttcaaaggcc gaggaatatg
      661 cccttttagc cttcttttgt caatggattt gtgcaaataa gcgccgttaa tgtgccggca
      721 ctcgttacgt agtgatggcg caggattcta ccagcttgca gggtgtgaat acagcttttc
      781 cgcgataaaa attgcagcag gcggtcactt tcttcccgtg atttgcgcca tggcaatgaa
      841 aagccacttc tttctgattt cggtactcaa tcgccggtta accttgaccg ctgtacaagg
      901 tatactcgga cgattttcac tgttttgagc cagacatgaa gctgatacgc ggcatacata
      961 atctcagcca ggccccgcaa gaagggtgtg tgctgactat tggtaatttc gacggcgtgc
     1021 atcgcggtca tcgcgcgctg ttacagggct tgcaggaaga agggcgcaag cgcaacttac
     1081 cggtgatggt gatgcttttt gaacctcaac cactggaact gtttgctacc gataaagccc
     1141 cggcacggct gacccggctg cgggaaaaac tgcgttacct tgcagagtgt ggcgttgatt
     1201 acgtgctgtg cgtgcgtttc gacaggcgtt tcgcggcgtt aaccgcgcaa aatttcatca
     1261 gcgatcttct ggtgaagcat ttgcgcgtaa aatttcttgc cgtaggtgat gatttccgct
     1321 ttggcgctgg tcgtgaaggc gatttcttgt tattacagaa agctggcatg gaatacggct
     1381 tcgatatcac cagtacgcaa actttttgcg aaggtggcgt gcgtatcagc agcaccgctg
     1441 tgcgtcaggc gctggcggat gacaatctgg ctctggcaga aagtttactg gggcacccgt
     1501 ttgccatctc cgggcgtgta gtccacggtg atgaattagg gcgcactata ggtttcccga
     1561 cggcgaatgt accgctacgc cgtcaggttt ccccggtgaa aggggtttat gcggtagaag
     1621 tgctgggcct cggtgaaaag ccgttacccg gcgtggcaaa cattggaaca cgcccaacgg
     1681 ttgccggtat tcgccagcag ctggaagtgc atttgttagg tgttgcaatg gacctttacg
     1741 gtcgccatat acaagtagtg ctgcgtaaaa aaatacgcaa tgagcagcga tttgcgtcgc
     1801 tggacgaact gaaagcgcag attgcgcgtg atgaattaac cgcccgcgaa ttttttgggc
     1861 taacaaaacc ggcttaagcc tgttatgtaa tcaaaccgaa acacggaacc gagaatctga
     1921 tgagtgacta taaatcaacc ctgaatttgc cggaaacagg gttcccgatg cgtggcgatc
     1981 tcgccaagcg cgaaccggga atgctggcgc gttggactga tgatgatctg tacggcatca
     2041 tccgtgcggc taaaaaaggc aaaaaaacct tcattctgca tgatggccct ccttatgcga
     2101 atggcagcat tcatattggt cactcggtta acaagattct gaaagacatt atcgtgaagt
     2161 ccaaagggct ttccggttat gactcgccgt atgtgcctgg ctgggactgc cacggtctgc
     2221 cgatcgagct gaaagtagag caagaatacg gtaagccggg tgagaaattc accgccgccg
     2281 agttccgcgc caagtgccgc gaatacgcgg cgacccaggt tgacggtcaa cgcaaagact
     2341 ttatccgtct gggcgtgctg ggcgactggt cgcacccgta cctgaccatg gacttcaaaa
     2401 ctgaagccaa catcatccgc gcgctgggca aaatcatcgg caacggtcac ctgcacaaag
     2461 gcgcgaagcc agttcactgg tgcgttgact gccgttctgc gctggcggaa gcggaagttg
     2521 agtattacga caaaacttct ccgtccatcg acgttgcttt tcaggcggtc gatcaggatg
     2581 cactgaaagc aaaatttgcc gtaagcaacg ttaacggccc aatctcgctg gtgatctgga
     2641 ccactacgcc gtggactctg cctgcgaacc gcgcaatctc tattgcacct gatttcgact
     2701 atgcgctggt gcagatcgac ggtcaggccg tgattctggc gaaagatctg gttgaaagcg
     2761 taatgcagcg tatcggcgtg accgattaca ccattctcgg cacggtaaaa ggtgcggagc
     2821 ttgagttgct gcgctttacc catccgttta tgggcttcga cgttccggca atcctcggcg
     2881 atcacgttac cctggatgcc ggtaccggtg ccgttcacac cgcgcctggc cacggcccgg
     2941 acgactatgt gatcggtcag aaatacggcc tggaaaccgc taacccggtt ggcccggacg
     3001 gcacttatct gccgggcact tatccgacgc tggatggcgt gaacgtcttc aaagcgaacg
     3061 acatcgtcgt tgcgctgctg caggaaaaag gcgcgctgct gcacgttgag aaaatgcagc
     3121 acagctatcc gtgctgctgg cgtcacaaaa cgccgatcat cttccgcgcg acgccgcagt
     3181 ggttcgtcag catggatcag aaaggtctgc gtgcgcagtc actgaaagag atcaaaggcg
     3241 tgcagtggat cccggactgg ggccaggcgc gtatcgagtc gatggttgct aaccgtcctg
     3301 actggtgtat ctcccgtcag cgcacctggg gcgtaccgat gtcactgttc gtgcacaaag
     3361 acacggaaga gctgcatccg cgtactctcg aactaatgga agaagtggca aaacgcgttg
     3421 aagttgacgg catccaggcg tggtgggatc ttgatgcgaa agagatcctc ggcgatgaag
     3481 ctgatcagta cgtgaaagtg ccggacacat tggatgtatg gtttgactcc ggatctaccc
     3541 actcttctgt tgttgacgtg cgtccggaat ttgccggtca cgcagcggac atgtatctgg
     3601 aaggttctga ccagcaccgt ggttggttca tgtcttccct aatgatctcc accgcgatga
     3661 aaggcaaagc gccgtatcgt caggtactga cccacggctt taccgtggat ggtcagggtc
     3721 gcaagatgtc taaatccatc ggcaataccg tttcgccgca ggatgtgatg aacaaactgg
     3781 gcgcggatat tctgcgtctg tgggtggcat caaccgacta caccggtgaa atggccgttt
     3841 ctgacgagat cctgaaacgt gctgccgata gctatcgtcg tatccgtaac accgcgcgct
     3901 tcctgctggc aaacctgaac ggttttgatc cagcaaaaga tatggtgaaa ccggaagaga
     3961 tggtggtact ggatcgctgg gccgtaggtt gtgcgaaagc ggcacaggaa gacatcctca
     4021 aggcgtacga agcatacgat ttccacgaag tggtacagcg tctgatgcgc ttctgctccg
     4081 ttgagatggg ttccttctac ctcgacatca tcaaagaccg tcagtacacc gccaaagcgg
     4141 acagcgtggc gcgtcgtagc tgccagactg cgctgtatca catcgctgaa gcgctggtgc
     4201 gctggatggc accaatcctc tccttcaccg ctgatgaagt gtggggctat ctgccgggcg
     4261 aacgtgaaaa atacgtcttt accggtgagt ggtacgaagg cctgtttggt ctggcagaca
     4321 gtgaagcgat gaacgatgcg ttctgggacg agctgttgaa agtgcgtggc gaagtgaaca
     4381 aagtcattga gcaagcgcgt gccgataaga aagtgggtgg ctcgctggaa gcggcagtaa
     4441 ccttgtatgc agaaccggaa ctggcggcga aactgaccgc gctgggcgat gaattacgat
     4501 ttgtcctgtt gacctccggc gctaccgttg cagactataa tgacgcacct gctgatgctc
     4561 agcagagcga agtactcaaa gggctgaaag tcgcgttgag taaagccgaa ggtgagaagt
     4621 gcccacgctg ctggcactac acccaggatg tcggcaaggt ggcggaacac gcagaaatct
     4681 gcggccgctg tgtcagcaac gtcgccggtg acggtgaaaa acgtaagttt gcctgatgag
     4741 tcaatcgatc tgttcaacag ggctacgctg gctgtggctg gtggtagtcg tgctgattat
     4801 cgatctgggc agcaaatacc tgatcctcca gaactttgct ctgggggata cggtcccgct
     4861 gttcccgtcg cttaatctgc attatgcgcg taactatggc gcggcgttta gtttccttgc
     4921 cgatagcggc ggctggcagc gttggttctt tgccggtatt gcgattggta ttagcgtgat
     4981 cctggcagtg atgatgtatc gctcgaaggc cacgcagaag ctaaacaata tcgcttatgc
     5041 gctgattatt ggcggcgcgc tgggcaacct gttcgaccgc ctgtggcacg gcttcgttgt
     5101 cgatatgatc gacttctacg tcggcgactg gcacttcgcc accttcaacc ttgccgatac
     5161 tgccatttgt gtcggtgcgg cactgattgt gctggaaggt tttttgcctt ctaaagcgaa
     5221 aaaacaataa taaaccctgc cggataaggc ctacagattg ctgcgaaatc gtaggccgca
     5281 tccggcaaaa atcctcaaaa tataagagca aacctgcatg tctgaatctg tacagagcaa
     5341 tagcgccgtc ctggtgcact tcacgctgaa actcgatgat ggcaccaccg ctgagtctac
     5401 ccgcaacaac ggtaaaccgg cgctgttccg cctgggcgat gcttctcttt ctgaagggct
     5461 ggagcaacac ctgttggggc tgaaagtggg cgataaaacc accttctcgc tggagcccga
     5521 tgcggcgttt ggcgtgccgt caccggacct gattcagtac ttctcccgcc gtgaatttat
     5581 ggatgcaggc gagccagaaa ttggcgcaat tatgcttttt accgcaatgg atggcagtga
     5641 gatgcctggc gtgatccgcg aaattaacgg cgactccatt accgttgatt tcaaccatcc
     5701 gctggccggg cagaccgttc attttgatat tgaagtgctg gaaatcgatc cggcactgga
     5761 ggcgtaacat gcagatcctg ttggccaacc cgcgtggttt ttgtgccggg gtagaccgcg
     5821 ctatcagcat tgttgaaaac gcgctggcca tttacggcgc accgatatat gtccgtcacg
     5881 aagtggtaca taaccgctat gtggtcgata gcttgcgtga gcgtggggct atctttattg
     5941 agcagattag cgaagtaccg gacggcgcga tcctgatttt ctccgcacac ggtgtttctc
     6001 aggcggtacg taacgaagca aaaagtcgcg atttgacggt atttgacgcg acctgtccgc
     6061 tggtgaccaa agtgcatatg gaagtcgccc gcgccagtcg ccgtggcgaa gaatctattc
     6121 tcatcggtca cgccgggcac ccggaagtgg aagggacaat gggccagtac agtaacccgg
     6181 aagggggaat gtatctggtc gaatcgccgg acgatgtgtg gaaactgacg gtcaaaaacg
     6241 aagagaagct ctcctttatg acccagacca cgctgtcggt ggatgacacg tctgatgtga
     6301 tcgacgcgct gcgtaaacgc ttcccgaaaa ttgtcggtcc gcgcaaagat gacatctgct
     6361 acgccacgac taaccgtcag gaagcggtac gcgccctggc agaacaggcg gaagttgtgt
     6421 tggtggtcgg ttcgaaaaac tcctccaact ccaaccgtct ggcggagctg gcccagcgta
     6481 tgggcaaacg cgcgtttttg attgacgatg cgaaagatat ccaggaagag tgggtgaaag
     6541 aggttaaatg cgtcggcgtg actgcgggcg catcggctcc ggatattctg gtgcagaatg
     6601 tggtggcacg tttgcagcag ctgggcggtg gtgaagccat tccgctggaa ggccgtgaag
     6661 aaaacattgt tttcgaagtg ccgaaagagc tgcgtgtcga tattcgtgaa gtcgattaag
     6721 tcattagcag cctaagttat gcgaaaatgc cggtcttgtt accggcattt tttatggaga
     6781 aaacatgcgt ttacctatct tcctcgatac tgaccccggc attgacgatg ccgtcgccat
     6841 tgccgccgcg atttttgcac ccgaactcga cctgcaactg atgaccaccg tcgcgggtaa
     6901 tgtctcggtt gagaaaacta cccgcaatgc cctgcaactg ctgcatttct ggaatgcgga
     6961 gattccgctc gcccaagggg ccgctgtgcc gctggtacgc gcaccgcgtg atgcggcatc
     7021 tgtgcacggc gaatcgggaa tggctggcta cgactttgtt gagcacaacc gaaagccgct
     7081 cgggataccg gcgtttctgg cgattcggga tgccctgatg cgtgcaccag agcctgttac
     7141 cctggtggcc atcggcccgt taaccaatat tgcgctgtta ctttcacaat gcccggaatg
     7201 caagccgtat attcgccgtc tggtgatcat gggtggttct gccggacgcg gcaactgtac
     7261 gccaaacgcc gagtttaata ttgctgccga tccagaagct gctgcctgtg tcttccgcag
     7321 tggtattgaa atcgtcatgt gcggtttgga tgtcaccaat caggcaatat taactcctga
     7381 ctatctctct acactgccgc agttaaaccg taccgggaaa atgcttcacg ccctgtttag
     7441 ccactaccgt agcggcagta tgcaaagcgg cttgcgaatg cacgatctct gcgccatcgc
     7501 ctggctggtg cgcccggacc tgttcactct caaaccctgt tttgtggcag tggaaactca
     7561 gggcgaattt acctcaggca cgacggtggt tgatatcgac ggttgcctgg gcaagccagc
     7621 caatgtacag gtggcattgg atctggatgt gaaaggcttc cagcagtggg tggctgaggt
     7681 gctggctctg gcgtcgtaac ctgtcacatg ttattggcat gcagtcattc atcgactcat
     7741 gcctttcact gatatccctc cctgtttatc attaatttct aattatcagc gtttttggct
     7801 ggcggcgtag cgatgcgctg gttactctga aaacggtcta tgcaaattaa caaaagagaa
     7861 tagctatgca tgatgcaaac atccgcgttg ccatcgcggg agccgggggg cgtatgggcc
     7921 gccagttgat tcaggcggcg ctggcattag aaggcgtgca gctgggcgcg gcgctggagc
     7981 gcgaaggatc ttctttactg ggcagcgacg ccggtgagct ggctggtgca gggaaaacag
     8041 gcgttaccgt gcaaagcagc ctcgatgcgg taaaagatga ttttgatgtg tttatcgatt
     8101 ttacccgtcc ggaaggtacg ctgaaccatc tcgctttttg tcgccagcat ggcaaaggga
     8161 tggtgatcgg cactacgggg tttgacgaag ccggtaaaca ggcgattcgt gacgccgccg
     8221 ccgatattgc gattgtcttt gccgccaatt ttagcgttgg cgttaacgtc atgcttaagc
     8281 tgctggagaa agcagccaaa gtgatgggtg actacaccga tatcgaaatt attgaagcac
     8341 atcatagaca taaagttgat gcgccgtcag gcaccgcact ggcaatggga gaggcgatcg
     8401 cccacgccct tgataaagat ctgaaagatt gcgcggtcta cagtcgtgaa ggccacaccg
     8461 gtgaacgtgt gcctggcacc attggttttg ccaccgtgcg tgcaggtgac atcgttggcg
     8521 aacataccgc gatgtttgcc gatattggcg agcgtctgga gatcacccat aaggcgtcca
     8581 gccgcatgac atttgctaac ggcgcggtaa gatcggcttt gtggttgagt ggtaaggaaa
     8641 gtggtctttt tgatatgcga gatgtgcttg atctcaatag tttgtaacca caaaatattt
     8701 gttatggt
//
LOCUS       DAZFJF010000164         8622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFJF010000164 DAZFJF010000000
VERSION     DAZFJF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.165"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..5248
                     /locus_tag="ACHKAU_004388"
                     /pseudo
     CDS             72..5248
                     /locus_tag="ACHKAU_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5471..5737
                     /gene="hipB"
                     /locus_tag="ACHKAU_004389"
     CDS             5471..5737
                     /gene="hipB"
                     /locus_tag="ACHKAU_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO5533328.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5737..7059
                     /gene="hipA"
                     /locus_tag="ACHKAU_004390"
     CDS             5737..7059
                     /gene="hipA"
                     /locus_tag="ACHKAU_004390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO5533329.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7371..7579
                     /locus_tag="ACHKAU_004391"
                     /pseudo
     CDS             7371..7579
                     /locus_tag="ACHKAU_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8011..8574
                     /gene="fimA"
                     /locus_tag="ACHKAU_004392"
     CDS             8011..8574
                     /gene="fimA"
                     /locus_tag="ACHKAU_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5533330.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         2359 a         1815 c         2318 g         2130 t
ORIGIN      
        1 ataagtttca tttttaatgt ctgacgactc atctctttcg tggctgtatt ttttgtatat
       61 ggagcgttta tatgaataga atctatcgcg tgatatggaa ttgcactcta caggtatttc
      121 aggcctgctc ggaattaact cgcagggcag gtaaaacatc gacggttaat ttgcgtaaat
      181 cctctggact gacaacgaaa ttcagtagat tgacgctggg tgttttgctg gcactaagcg
      241 gttcagcgtc tggtgcaagt ctggaagttg ataatgatca gattaccaat attgatactg
      301 atgttgctta tgatgcctac ctggttggct ggtatggcac tggagtgctt aatattttgg
      361 ctggcggtaa tgcctcctta accactatta ctaccagcgt cattggcgct aatgaggact
      421 cagagggcac cgttaatgtt ttgggtggca cctggcgatt gtatgatagc ggaaataatg
      481 caaggccttt aaatgtgggt caatccggaa cggggacgct gaatattaaa cagaagggtc
      541 acgtcgatgg aggctattta agattaggtt cttcgacagg aggcgtcggg acggtcaatg
      601 ttgagggaga ggactctgtt ctgacgaccg aattattcga aatagggagt tacggtacgg
      661 gctcattaaa tattacggat aagggttacg tcacgagttc aatcgtcgcc attttaggct
      721 atcaggcggg cagtaatggc caggttgtcg ttgaaaaggg tggcgagtgg ctaataaaaa
      781 ataacgattc ctcaattgaa tttcaaattg gtaatcaagg aactggggag gcgactattc
      841 gcgagggtgg actgattacg gctgaaaata cgattatcgg tggcaatgcc accggtgtcg
      901 gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc tataatggat
      961 atttcggtaa tggcacactc aatatttcca ataatggact aattaataac aaagaatatt
     1021 cattagtggg cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat aaagggcatt
     1081 ggaatttcct cggaacgggc gaagctttcc gctatatcta tatcggtgat gctggcgacg
     1141 gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact gcggggatga
     1201 aagaaacagg cacaggcaac attactgtta aggataagaa ctccgttatc actaatctcg
     1261 gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgaa gggcttgttg
     1321 tcagcaacgg aggaagctca ctcggttatg gagaaaccgg cgtcgggaat gtcagcatca
     1381 ccacgggggg gatgtgggag gtcaataaga atgtctatac caccattggt gttgcgggcg
     1441 tcggaaacct caatattagc gatggcggta agttcgtatc gcaaaatatt acttttttgg
     1501 gcgataaagc aagcggtatc ggcacactga acctgatgga tgcgacatca tcgttcgata
     1561 ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt aatggtgcca
     1621 cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggtaag gggatagtta
     1681 atatttcaac ggacagtctc tggaatttaa agacatcatc tactaacgca caattgctac
     1741 aggtcggtgt attaggcaag ggtgaactga atattaccac cggaggtata gttaaagcgc
     1801 gtgatacaca gatagctctc aatgacaaaa gtaagggcga cgtgagggtg gatgggcaga
     1861 actctcttct tgaaacattc aatatgtacg tagggacatc tggtacgggt acgttaaccc
     1921 tgacgaatag cggcacgctg aatgtcgaag gtggagaagt ttacttaggt gtttttgaac
     1981 ctgctgtagg aacgctaaac attggtgcag ctcacggtga ggcggcggca gatgccgggt
     2041 atataaccaa cgcgacgaaa gtagagtttg gttctggcga aggtgttttt gtctttaatc
     2101 atactaataa cagtgatgcc ggctaccagg tcgatatgct gattacaggt gacgataaag
     2161 acggaaaagt gatccatgat gcaggccata cggtgttcaa tgcagggaat acttatagcg
     2221 gtaaaacgct ggtcaatgac ggcctcctga ccattgcgtc tcatacggca gatggggtaa
     2281 cgggcatggg ttcgagtgaa gtcaccattg caagccccgg tacgctcgac attctcgcat
     2341 caacgaacag tgcaggagat tacacgctga ccaatgcgct caaaggcgat ggcttgatgc
     2401 gagtgcagct gtcatcctac gacaagatgt ttggctttac ccatgcaaca gggactgaat
     2461 tcgccggtgt tgcccaactg aaagacagta ccttcactct ggaacgcgac aacaccgctg
     2521 cgcttactca cgcgatgttg cagtctgaca gtgaaaatac cacatcggta aaagtgggag
     2581 agcaatccat tggtggactg gccatgaatg gcggtacgct cattttcgat acggatattc
     2641 ctgctgcgac gcttgcagag ggatatatca gcgtcgatac gctggttgtc ggcgcgggtg
     2701 actacacctg gaaaggccgt aactatcagg taaacgggac gggcgacgtg cctaaaccgt
     2761 ggaatgatcc catggcgaat aaccctctga cgacgctcaa tttgctggaa cacgatgata
     2821 gccatgtcgg cgttcaactg gtgaaggcgc aaacggttat tgggtcgggt ggctcattaa
     2881 cgttacgtga tttacagggc gacgaggtgg aagcggacaa aacgttacac attgcgcaaa
     2941 acggaacggt ggtcgccgag ggtgattatg gattccgcct cacgaccgca ccaggtgatg
     3001 gtttgtacgt taactatggg ctgaaagcgc tgaacatcca tggtgggcaa aagctgacgt
     3061 tagccgaaca tggcggagcc tatggcgcaa cggccgatat gtcggcaaaa atcggtggtg
     3121 aaggggatct ggcaatcaat acggtgcgac aggtttcgct ttccaacggt cagaacgact
     3181 atcagggggc aacctacgtt cagatgggga cattacgtac cgatgcggat ggtgcacttg
     3241 gcaacacccg ggaactgaac atcagcaacg cggccatcgt cgatcttaat ggatcgacgc
     3301 agacggtaga gacattcacc gggcagatgg gttcgactgt tttgttcaaa gagggggcgc
     3361 tgacggtaaa taaaggtggg atcagtcagg gtgaactgac aggtggcgga aacctgaatg
     3421 ttacaggggg aacgctggct atcgaggggc ttaatgcacg ctacaatgcg ttaaccagca
     3481 ttagcccaaa tgcggaagtc agcctcgata atacgcaggg gttaggcaga ggaaatattg
     3541 ccaataacgg tctgttaacg ctaaaaaacg tgactggcga actgcgcaat agcataagcg
     3601 ggaagggtat cgtgagcgca accgccagga cagatgtaga gctggatggc gataatagcc
     3661 gctttgtggg gcaattcaac attgatacag gcagcgcgct cagcgtcaac gagcagaaaa
     3721 acctgggtga tgcttccgtt atcaataatg gcctgctcac catctccact gagcgtagct
     3781 gggcgatgac gcacagtatc agcggtagcg gtgatgtgac aaaactgggt accgggatcc
     3841 tgactcttaa caacgattcc gcggcgtatc agggtacgac ggatatcgtg ggggggggaa
     3901 attgctttcg gttccgactc tgccattaat atggcaagtc aacacattaa tatccataac
     3961 agcggtgtga tgtcgggaaa tgtcaccact gcaggtgatg tgaacgttat gcctgggggg
     4021 acactgcgtg tcgctaaaac cacaatcggc ggcaacctgg agaatggcgg cacggttcaa
     4081 atgaatagcg aaggggggaa accggggaat gtactgaccg ttaacggcaa ctataccgga
     4141 aacaatggcc tgatgacgtt caacgcgacg ctgggcggcg ataattcgcc caccgataaa
     4201 atgaacgtga aaggcgatac ccaagggaac actcgcgttc gggttgataa cattggcggc
     4261 gtcggtgcac aaacggtcaa cggtattgaa ctcattgagg ttggcggtaa ttctgcaggt
     4321 aacttcgcgc tgaccaccgg aactgtcgaa gctggggctt acgtctacac gctggctaaa
     4381 gggaagggga atgacgagaa aaactggtat ctgaccagta aatgggacgg cgtaacgcca
     4441 gcggatacac ccgatcccat caataatccc cctgttgtgg atccggaagg cccatcagtt
     4501 tatcgcccgg aggccggaag ctatatcagc aacattgccg cagccaactc gctgtttagc
     4561 catcgtttac acgaccgtct gggtgagccg cagtatatag attcactgca ttctcagggg
     4621 tcggcaagca gtatgtggat gcgtcatgtc ggagggcacg aacgttcaag ggccggtgac
     4681 ggtcagctaa ttactcaggc taaccgctat gtattgcagc taggcggcga tttggcgcag
     4741 tggagtagca acgcgcagga tcgctggcat gacggcctct gttgagggtg ggtatacctt
     4801 tgaagcggga acatttagag gcagcgaagg gacgctgaat acctggtacg tccagccaca
     4861 ggcgcaaatc acctggatgg gtgtgaaaga ttctgaccat aatcggaaag acggaacacg
     4921 cattgaaacg gaaggcgacg gaaacgtgca aacgcgactt ggggtgaaaa cctacctgaa
     4981 tagccatcac aagcgtgacg atggtaaaca gcgtgagttc cagccttaca ttgaagcgaa
     5041 ctggatcaac aatagcaaag tctacgccgt gaagatgaat ggtcaaaccg taagccgtga
     5101 tggtgcgcgt aatctcggtg aagtacgtac cggggttgag gcgaaagtaa ataacaacct
     5161 tagcctgtgg gggaatgtcg gtgtgcaact aggtgataaa ggctatagcg atactcaggg
     5221 catgctggga gtgaaatata gctggtaaac cgtataagcc gcatgtcgag atggcatgcg
     5281 gcttaatatt gccgacttca aacggcgcat cagcgccttt tttaaatcct cctttttatc
     5341 cgcgatcgcg gatatcgcag cgtttatccc gtagagcgga taagacgtgt ttccaaattg
     5401 acttatcctc actaaaggat aaaactgata atatcctctt atgcggataa atttgctgtg
     5461 gacgtatgac atgatgagct ttcagaagat ctatagccca acgcaattgg cgaatgcaat
     5521 gaaactggtt cgccagcaaa atggctggac gcagagcgag ctggcgaaaa aaattggcat
     5581 taagcaggcg acaatttcca atttcgaaaa caaccctgac aataccacgc tcacgacatt
     5641 ttttaagatt ttacagtcgc ttgaactctc aatgacacta tgcgacacga aaaatgcctc
     5701 gccagaatca acagaacagc aagatctgga gtggtaatgc ctaaacttgt cacttggatg
     5761 aacaaccagc gggtaggcga gttaacgaag ttagccaacg gcgcgcacac ctttaagtat
     5821 gcaccggagt ggttagccag ccgttatgcc agaccgttgt cactttcgct gccattgcag
     5881 agggggaata tcacctcaga tgccgtattt aacttcttcg ataacctgtt acccgatagc
     5941 ccgattgtac gtgaccggat cgttaaacgt tatcatgcta aatccagaca accgtttgat
     6001 ttattgtcag aaatagggcg agacagcgtt ggtgccgtaa cgttattacc cgaaaatgaa
     6061 accataacgc gcccgataat ggcatgggaa aagcttactg aagccaggct tgaagaagta
     6121 ttaacggctt ataaagcaga tatccctctg ggcatgatta gagaagaaaa tgactttcgc
     6181 atctcggttg ctggcgcgca ggagaagaca gcgctgctta gaataggcaa tgactggtgc
     6241 attccgaaag gaataacgcc gacgacgcac atcattaaat taccgattgg cgaaatcaga
     6301 cagcccaatg cgacgctcga tctcagccaa agcgttgata atgagtatta ctgtctgctg
     6361 ctggcgaaag aacttgggtt gaatgttccg gacgcagaaa tcattaaagc gggaaatgtg
     6421 cgcgcgttag cggtcgaacg ttttgacagg cgttggaata ctgagcgaac ggttttactt
     6481 cgcttgccac aggaggatat gtgtcagaca ttcggtttac cttcatctgt gaaatatgaa
     6541 tcagatggag gcccaggcat cgcgcggatc atggcgtttt tgatggggtc cagcgaggcg
     6601 cttcgcgatc gttatgattt tatgaaattc caggtcttcc agtggttgat tggcgcaacg
     6661 gatggccatg caaaaaactt ctccgtattt attcaggctg gcggcagtta tcgactcacg
     6721 ccattttacg acatcatttc agcatttccg gtccttggcg gtacgggaat acacatcagc
     6781 gatctcaaac tggcaatggg gcttaacgca tccaaaggca aaaaaacggc aatcgataaa
     6841 atttatccgc gacatttttt ggcgacagca aaggtgctga gattcccgga agtgcagatg
     6901 catgaaatcc taagtgactt tgccagaatg attccggcag cactggataa cgtgaagact
     6961 tcattaccga cagattttcc ggagaacgtg gtgacggcag ttgaaaccaa tgtgttgagg
     7021 ttgcatggac ggttaagccg agaatacggt agtaagtgag atatggggtt tggtcgttgt
     7081 taatgagcac gacaatcatg accgccaacg actggattct tccttggttt atactggtgc
     7141 gcataatgta tattatgtta aattatgtct gagtgatgcg aagtgctttt ctggtagtcg
     7201 ttattcgttc agggttcaag aacttaagcc tctcctcttt cataattggc ctgtgcatgg
     7261 agaaatagcc tggttattcc ctgccccatg ctaagagcgc agtgtctgtc gcatagacac
     7321 tgcatacgaa aacaccagag ggtgctgtta cttatttaag gacggatcaa gcgggaatga
     7381 gtccactccg gtatcagaaa tggctacgtc tcaatgaagt caggcgaccg atgctgaatg
     7441 aacattacga tgtcaccaca gcagcctatg ctgtcggtta cgaaagctat cccatttcag
     7501 tcgggaatat tcgcggatgt ttggagagtc acccaagaga gatattacca ggttaagaaa
     7561 gtctgtcggt cagctttaat aaaccctgca cttatctgtt tagagcaatt cggtgttagt
     7621 ttcagcaagc aaacattaac catagctaat gatttatagc catattaacc attggtagcc
     7681 cttgaccttt cctgacactt cccttgttcg caataatatt ctgtttagtc acggtttata
     7741 aataacatgc ataatgaaat cgtcatgtta tttagcgaca taaaatgttt ttgcaaatac
     7801 tctcttcaac aagtgcacaa taaacttgtt gtgattaggt gtccttgtga tttaaagata
     7861 ttatcgttct tcgtgaaatg taataaccct ggaaactata ttctcagggc aacaaccaat
     7921 caagaatgat tcttgttttt aagcaataac agaatgactt aattgttaac cctctttatt
     7981 cagctactta aatataaatt ttggagaatt atgaaactca aacatgttgg tatgattgtc
     8041 gtttctgtgt tggcgatgtc gtctgctgcg gtaagcgcag ccgagggtga tgaatcagta
     8101 acgaccactg ttaatggcgg tgttattcat tttaaaggtg aagtggtaaa tgccgcttgt
     8161 gcaattgatt cagaatcaat gaaccaaacg gttgagctgg gtcaggttcg ttcttctcgc
     8221 ttggctaaag cgggtgacct cagctccgcc gttggcttca atatcaagct gaatgattgt
     8281 gataccaatg tttccagtaa tgcagctgtt gcattcctgg gtactactgt caccagtaat
     8341 gacgatacgt tagcgctgca gagttcagcg gcaggctctg cccaaaatgt cggtattcaa
     8401 attttggacc gtacgggtga ggtattaata cttgatgggg ccacttttag tgctaaaacc
     8461 gacttgattg atggcacgaa tatactgcca ttccaggctc gttatattgc tctcgggcag
     8521 tccgtagctg gtactgcaaa cgctgatgcg accttcaaag ttcaatatct ataattcaat
     8581 cttaaggtgg ctatacgtca tctgaaattg aaacagggtg tg
//
LOCUS       DAZFJF010000165         8596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFJF010000165 DAZFJF010000000
VERSION     DAZFJF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.166"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            30..166
                     /locus_tag="ACHKAU_004393"
     ncRNA           30..166
                     /ncRNA_class="other"
                     /locus_tag="ACHKAU_004393"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(257..943)
                     /locus_tag="ACHKAU_004394"
     CDS             complement(257..943)
                     /locus_tag="ACHKAU_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO5533331.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1524..2312)
                     /gene="phoH"
                     /locus_tag="ACHKAU_004395"
     CDS             complement(1524..2312)
                     /gene="phoH"
                     /locus_tag="ACHKAU_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO5533332.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2931..4202)
                     /gene="efeB"
                     /locus_tag="ACHKAU_004396"
     CDS             complement(2931..4202)
                     /gene="efeB"
                     /locus_tag="ACHKAU_004396"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO5533333.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(4208..5335)
                     /gene="efeO"
                     /locus_tag="ACHKAU_004397"
     CDS             complement(4208..5335)
                     /gene="efeO"
                     /locus_tag="ACHKAU_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO5533334.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5393..6223)
                     /gene="efeU"
                     /locus_tag="ACHKAU_004398"
     CDS             complement(5393..6223)
                     /gene="efeU"
                     /locus_tag="ACHKAU_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO5533335.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(6889..8397)
                     /gene="putP"
                     /locus_tag="ACHKAU_004399"
     CDS             complement(6889..8397)
                     /gene="putP"
                     /locus_tag="ACHKAU_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO5533336.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
BASE COUNT         2234 a         2376 c         1983 g         2003 t
ORIGIN      
        1 ctcgtacgaa agtacgggct ttttttcgta tattgcacac accgggggga tgagaagccc
       61 cgaccggggt tcgacaactg gcgacagcca gttggacaga ccgtgaacgc agtgagcggg
      121 ctgcccgcag ggcgagcgaa gcgagtcaat ccccccctca ccgccatctt taaagaagag
      181 ctcgtacgaa ggtacgggct ttttttcgta tattgcacac accgggggga tgagaagccc
      241 cgaccgggat gcttagttaa tgcaccggtt cactctttgt cgccgcgact aatgggcgaa
      301 ttttgtcacg agtctcccag accggaggaa tttcctccaa tttagtgaca gaagctcgta
      361 caaaaggcac ccgtgcaaga gtcataattg tatccaacca acggtcacga ataatgcgat
      421 ccgccctttc atgactcggg tcatccagct caataacaca gactatttta ttgttcttct
      481 ttgaaaccag aacataatcc attgccatgc cattataaat tgcaaagaaa cgctttcttt
      541 cttcttcgct aaactcaggt tgttctggaa agttgatcaa gcgccccaga gagacctggc
      601 aatggatatg acaataatgt ccaaaccatt tttccagatt atcgaaatac attttttcac
      661 aacgcgtgat taaccactct tttctttcca ataaaatacc ttcggcaacc attccctgaa
      721 ataactcatc tcgctcttgc caacgagagt cacgataggt tttctgcggt atatagctcc
      781 ttgtcgacgc cttagcggca ggggaagttg cagatgaagg gacgggttgc gatttagcga
      841 tgattaccga tgaggcaact tttcggagtg tcaattttct ggcaacagca aaaccggcga
      901 taaatgcaat tgtcaaacaa gttacaaaag ccataaagct cataattaat tttcacctat
      961 ccatgttgat tatatttcat taccaatgtt actcgctgca gcgtgttaca tgccaacaat
     1021 gcaacgatca atatatagtt agtatcatgt tttgattatt acaaccatcc ctatttagcc
     1081 attgcggctt gccttccaca aaaaaataac aacacacaat attagcaaag aattaattaa
     1141 gattaacctt acatttataa tattatccct aaagaataaa tcagaggaat atattcggtg
     1201 ttaaacagtc agtcaaagta tagcccattg ttgattaaac ctcataagca aaattggcac
     1261 cagatctcct gtacaggttg aatggccgaa aaaagatagg cagatgctca tttttttggc
     1321 ttctggaaga aaaactctcc gcgctggcag tattacactg gtctgatgct atgacgaaaa
     1381 atgcctggta agagtatcta aaccagactc cagttcagct tatcaacacg ctcaaagtcg
     1441 cccccctgaa cgctaacctg aagtgaaaat ctgttgaaat aaaacaaagc ccggttcgcc
     1501 cgggctctgc accgataata cacttaactg taggcatgca gcgtacgttg gcaaagtgcc
     1561 gaacgtacgc agtcctcttt accgaaccgg acgatcccaa ccatttcatc ttcttcgaaa
     1621 cgttccagcg cgtcacttaa tccggagcac acgccgcgag gcaaatcgca ttgcgtgata
     1681 tcaccgttga cgataaccgt cacgttctcc ccgaggcggg ttaaaaacat tttcatttgc
     1741 gcggcagtca cattctgcgc ctcgtcaaga atgacgactg cattttcaaa ggtacgtcca
     1801 cgcatatagg cgaacggcgc aatttccacc ttccctattt ccggtcgcag gcagtactgc
     1861 ataaaggaag cccctaagcg ccggaccagc acgtcgtaga ccgggcgaaa atagggagca
     1921 aacttttctg cgatatctcc aggtaagaag ccaagatctt catcggcttg cagaactgga
     1981 cgggtgacga taatcctgtc gacatcctta tgtatcaggg cttctgccgc ttttgctgcg
     2041 ctgatccagg tttttccgca cccggcttcg cccgtggcga atatcagctg cttactctca
     2101 atagccttca gatagtgcaa ttgcgcttca tttcgcgcga ggatgggcga agtatcgcga
     2161 ctgtcgcggg ccataccaat ggcttctacg ccgcccatct gcacaagcga ggtgaccgat
     2221 tcttcttcac gctgcttatg gctgcgcgaa tcccgtctca gcacacgttt tgcctcgcga
     2281 cgagctttga tcactgcttt ttgtcttccc atggagagca ccttgagttg tttgtattca
     2341 tcacacgcgc cgttggcagc gcgattatgc gcacgaacat cagagggttg gcttccttgt
     2401 aagccatagt ttgcttttgg ataaaatgcc gaaaaacggc tacgcacacc gtttgcggcg
     2461 tcggtaacac gagtaaagag aggaagtgag aaaaatacag agtgacgaag ttgctaccgg
     2521 aggagaaatc tccgcaagtg gtggagcgta gcttaacaag acattgtcca gaacaggacg
     2581 ttaccatccg cgatctccat agtgactgac tatcactgcc gggaacttcc gctgctactt
     2641 aataattaca acagatctcg cattcattgc aacaatatat tcacttatat ttaaccataa
     2701 aacaccattt cagtgacatt agtttctact ggaaagatga cagagtgatg acagatatga
     2761 aaaaagctgt gtgctttcag caggattcgc atcaggcatc tgcgcacgaa cgccggatgc
     2821 ggcgtgaacg ccttatccgc ctaccgaacc cgcaccgatt gtaggcataa gaagacgcgc
     2881 aagcatcgca tcaggcatct gcgcaagaac gccttatccg tctataaaca ttaaacccgc
     2941 aataacgcgc ttccgagata atcgttcgcg tccttcactc ccggcagcgc aaaaaaataa
     3001 ccgccgccga taggtttaac gtattcctcc agcgcttcgc cattgagcct tttttgtact
     3061 gtcaggaagc ctttttccag atcgtgttgg taacagacaa acagtagccc catatccagt
     3121 tgcccggagt tggtgacgcc cagtgaataa ctgtagccac gacgcagcat caggctggac
     3181 tcactctccg ccgtgcgggg attcgccagc cggatatggc tgtccagcgc aatcaccttc
     3241 ccttccggat cgctggcgta atcgggaaca tcatgctcat gctgcattcc cagcggcgca
     3301 ccggtttgct tatcacgacc aaaaatcgtc tgctgttctt tcagcggagt tctgtcccaa
     3361 aattccactc gaaactgaat caagcgtact gcctgatagc tgccaccgat tgtccacgct
     3421 ggctcctgct gatctgccgt tacccacacc actttttgca tcaacttatc attctggcta
     3481 tcgggattgg cagtgccgtc tttgaaaccc agcaaattaa tcggcgtctc tttgccttta
     3541 ctacgcgccg cgtgatcgga aataaaccct tcccgcttcc agcgcacgct gagcaaatcc
     3601 ggcgtatgtt tgatgatatc gcgcagtgca tggatcaccg tgtcctgggt gttggcgcaa
     3661 atttgtagca acacatcacc atgacataac gccgcatcca gcgagtcgtt gggaaaacgc
     3721 gtcatcttct gcaacttttt cggcatctgt ggcgcaaggc caaagcgctc atcaaacaat
     3781 gagtgaccca ccgataacgt gatggtgaga ttatcgggcg caatgtagcc gccaagaatg
     3841 ccggaatcga gtggtggcag gcgcggattt ggcgtttctg gtgccgctcc gccctgagtc
     3901 agaaaagcaa aacgctgagt caacaagcga aacaaccgtt caagatcggc tttatcgctg
     3961 gcaagcacat caaacgccac cagcatcatt gcggcctgtt gtggcgtcag gatccctgcc
     4021 tgatgctcac cataaaacgg ctgtttctca ttgcgtgcat ccggtgaaag cgtacccggc
     4081 gcactttgcg ttttttgtgc atgagcgacc ggacaacttc ccgccagcgc cagcgcacca
     4141 atgcctttca gtaaacgtcg gcgtgacggt tcattcacgc cgtttttatc ttcatactgc
     4201 ataatgctta atccaatccc agcacaccgc gaagttgcgc cagatcttcc gccagcgcag
     4261 taatcggtcc tttcagtgca ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac
     4321 cgtctttagt acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt
     4381 ttgccaacag ttccgggttg gctttttgca gttgtggacg cagcagatcg acaattttct
     4441 gcgagccttc aacgttagcc tggaaatccc acagatcggt gtggctgtag cgatcttctt
     4501 caccgctgat tttgctggct gccacttcct caatcagtcc ggctgcaccg ccgaccactt
     4561 ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa atcgaccaca tcggtataaa
     4621 gctggtcagc gtactgatcc atgcctttgg tggtgttgtc gccaaacaat gctttttcca
     4681 gacggtggaa accggtgaat tttggatcag cggctttttg ctcgtaatca tcttcacggg
     4741 cgtcaatgct gccatccaga tcggagaaca gttcagcaat cggttcaatg cgctcatagt
     4801 gctggcgcgt cggcgcatac agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt
     4861 cggtaaaggc tttggtgtcg gtcaccagct gcgtggtttc cgccatgaca tacgctttat
     4921 atgcagtaat tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg
     4981 cctcaccttt gacgatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcgt
     5041 attcgccagg ctgtaaattc gccgtcattt tctggctaaa accaggggcg atattttccc
     5101 gctcttccac caccatcacg cctttgagga tctcccactc cagcgccttc tggctatggt
     5161 tctgaataat gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct
     5221 tatccgtcac ggtcactttg acctgcgtca catcagcggc gttagccata aaagcagaag
     5281 aaaacagcgc agccacgctc aactgcaatg cgttacggcg gaagttaatg gtcatgacga
     5341 gactatccct ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg
     5401 agcgagacgc tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga
     5461 gataaataaa ccagacggcg acttcgctga cgctcggcgc ttcctgatag ccaaaaatcc
     5521 cttccatcag cgtgccaaac agcgagtgag ttgagagcac cgcactcata tcgaaggcga
     5581 tttcctgaaa gtggttccac aatccggctt catgaaatgc gcgaatggca ccagctgcca
     5641 gccctgcggc gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga
     5701 ggcgaatacc gccccagtag agcaagaagc ctagcaccac ggcagtagca agaccgagca
     5761 ttgcacccag cggcggccag atcccgacat cttgttgaaa tgccgccagc aggaaaaaga
     5821 ccgactccag cccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat
     5881 gatgatttcc acgctgcaat gcgctatcga ctgcctgctc cagttgtact ttgacgttgc
     5941 gcgacacttt gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca
     6001 cgataccttc aaacagttcc tgctcttttt gcggaaattc gccggtggtt tcgttaatga
     6061 agatccccaa gcccaggcac aacgcagcgg caagcaacac gccaatccac atcacaccaa
     6121 tccatcggcc tcgctgggta cgcttaagat agctggcaat caaactgaca atcagcgcgg
     6181 cttcaagtcc ttcgcgcaac ataatgagaa acggaacaaa catgccaccc atccttaaac
     6241 gtttttctga gtcaatctat gcaaagaaaa gtaaatcgca gtgatagcga ttatcattac
     6301 ggtaaagaaa aatacaagcg gaatgtttat aaaatgatgt tgaaatgtaa acaaagagag
     6361 gtaatgccag gggatataaa cgattattcc aggtacaaag atgggattgt ttacaacgag
     6421 ccatgtgttc ttgccggatg ccgcgtaaac gccgtatcca gccgacaaaa ccgtcctgac
     6481 tcaatacatc aaaacttcca tgtaaaactg ataagcgggt gtatcaagca atttcgtcca
     6541 tcctcgatta tcaaatctaa ctttttgccc ggaacataag aacagcgcgg gtgttggtgg
     6601 cgtcaaaatc gctgaaacgt tccccgaagg ccggggcagc ccacatggat gtgggctgag
     6661 ggcgcgtctt acaggacgtt acctcgcgcc cgacccggta gccgaagggg ataagctgaa
     6721 ggtaccacgc agcggcgatt ttgttcgcca gagcccgggg gtgcaggggg ccgcggcgat
     6781 tggccgcccc ctgcgcgctc cctgcaccat tcaatttata tggcgaggca cattaaggga
     6841 gcgcaacccg ctgctgaagc caaacaaaac cgccgcaggc taagtccctt agctttcctg
     6901 caaccgtgac ggcggagccg aatgatagtg cgcatcggcc tcggcaaagc gtttttgcat
     6961 cgccgctgac ggcgctttac ccagcaaact aaacactaca atcccgatac tgccgaagat
     7021 gaagcccgga ataatttcgt agagtcccag ccagccgaac tgcttccaga cgataaccgt
     7081 cagcgcaccg atgatcatcc ccgccagcgc accgttgcgc gtcatgcgtg accacatcac
     7141 cgagaacagc accaccggac caaacgccgc gccaaagcct gcccacgcgt agctcactaa
     7201 gcccagcacg cggttttccg ggtttgccgc cagcgcaatc gccaccagcg ccaccaccag
     7261 caccatcaca cgtcctaccc acaccagctc tttctggctg gcctgtttac gcagaaacgc
     7321 tttgtataaa tcttcggtaa ttgcactgga acataccagc agctggcaac ttaaggttga
     7381 cattaccgcc gccagaatcg ccgacagcag aatcccggca atccacgggt taaacagaat
     7441 ttgcgccagt tcgataaaca cgcgctcggc gttctggttt accgcgcctg ctaccgccgg
     7501 gtgctcatta aagtaagcaa tcccaaagaa gccaacagcc accgcccctg ccaggcagag
     7561 gatcatccag gtcatactga tacgacgcgc atggacaatg ctgtggtgag agtctgccgc
     7621 cataaaacgc gccaggatgt gcggctgccc gaagtaaccc agcccccaac ccatcagtga
     7681 gataatggcg acaaagttca gccctttgag catatcaacg ttttcgatgc tcttttgttt
     7741 gatcacttcc agcgagtcac caaagccacc gacactgata atgacgataa ccggcgtcag
     7801 gatcagggca aaaatcatca ggctggcctg tacagtgtca gtccagctca ccgcgaggaa
     7861 accgccaata aaggtgtaaa ggatcgtcgc cgcagctccc gcccacagag ccgtttcgta
     7921 gctcatgcca aaggtacttt caaacagacg cgcgcctgcc acaatgcccg aagcgcaata
     7981 aatggtgaag aacagcaaaa tcaccagcgc ggagataatg cgcaaaatgc ggcttttatc
     8041 ttcaaagcgc ccggtgaaat aatccggcaa tgttaaggcg ttattgttgt attcggtatg
     8101 cacacgcaac cgccccgcca ccagcttcca gttgatccac gcgcctaatg tcaggccaat
     8161 ggcgatccaa ctttcggaaa tcccggaaag aaaaaccgcg cccggcaacc ccattaacag
     8221 ccagccgctc atatccgacg cacccgccga taatgccgtc acgaatggcc caaggctacg
     8281 accgcccaga atatagtcgt caaagttttt cgttgatcgc caggcgataa acccaatcaa
     8341 tatcatgcca aagatataga cacaaaatgt caccaacatc ggtgtgctaa tagccatcta
     8401 aagtctccaa aaaattatta tcggcaatgt cgaaacttgc cgttatatct gccaccggaa
     8461 cggggtaaca gagtttatgt tttaccaggg cgaccgtatc ctgccggaag cgctggttat
     8521 tcacaatcga tttaacacac catttacatt aaattttagt gctcagcgac actatttttc
     8581 atcaggttgc actctc
//
LOCUS       DAZFJF010000166         8466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFJF010000166 DAZFJF010000000
VERSION     DAZFJF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.167"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(54..260)
                     /locus_tag="ACHKAU_004400"
     CDS             complement(54..260)
                     /locus_tag="ACHKAU_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO5533337.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            573..662
                     /gene="kdpF"
                     /locus_tag="ACHKAU_004401"
     CDS             573..662
                     /gene="kdpF"
                     /locus_tag="ACHKAU_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO5533338.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            662..2335
                     /gene="kdpA"
                     /locus_tag="ACHKAU_004402"
     CDS             662..2335
                     /gene="kdpA"
                     /locus_tag="ACHKAU_004402"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO5533339.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2358..4406
                     /gene="kdpB"
                     /locus_tag="ACHKAU_004403"
     CDS             2358..4406
                     /gene="kdpB"
                     /locus_tag="ACHKAU_004403"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO5533340.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4415..4987
                     /gene="kdpC"
                     /locus_tag="ACHKAU_004404"
     CDS             4415..4987
                     /gene="kdpC"
                     /locus_tag="ACHKAU_004404"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO5533341.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4980..7664
                     /gene="kdpD"
                     /locus_tag="ACHKAU_004405"
     CDS             4980..7664
                     /gene="kdpD"
                     /locus_tag="ACHKAU_004405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO5533342.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7661..8338
                     /gene="kdpE"
                     /locus_tag="ACHKAU_004406"
     CDS             7661..8338
                     /gene="kdpE"
                     /locus_tag="ACHKAU_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO5533343.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1785 a         2184 c         2451 g         2046 t
ORIGIN      
        1 aaaaaccgcc agcgtttccg ctggcggttt tcaatgtatt caaaactcag caatcaataa
       61 aaatcaccag ttgccttttc ggcctgatcc atccacaccg gtttatcgct ggttttcgac
      121 cagacgcgat gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaag catgaaaggc
      181 agcgccagaa cgcccgctgc aaccgcataa gtacggcgta aaaagataag ccatgcagga
      241 tattctctgt agagttccat acttttctcc cccttttgta tctacccggt gaatggtacc
      301 ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc ttttgtgtaa ttttactact
      361 catccgacca cttatttttg cttattgatg gtttatttac attcatcctg taattaagtt
      421 acacaaaagt taaattaata ctaaacatta gttaaatcat ggcttttgcc atttttatac
      481 tttttttaca ccccgcccgc agatttttgc gaaatctttg cagccagaat tctacccttc
      541 cggtatcact tttaggccac tggaggtgca ctgtgagtgc aggcgtgata accggcgtat
      601 tgctggtgtt tttattactg ggttatctgg tttatgccct gatcaatgcg gaggcgttct
      661 gatggctgcg caagggttct tactgatcgc cacgttttta ctggtgttaa tggtgctggc
      721 gcgtccttta ggcagcgggc tggcgcggct gattaatgac attcctcttc caggtacagc
      781 gggcgttgaa cgtatactct ttcgtttacc tggcgtctct gaccatgaga tgaactggaa
      841 gcaatatctt tgtgccattc tcggcctgaa catgctgggg ctggcggtgc tgttttttat
      901 gttgctcgat cagcactatc tgccgcttaa tccacagcag ttgccggggc tgtcgtggga
      961 tctggcgctg aatgccgccg tcagctttgt cacgaatacc aactggcaat cttacagcgg
     1021 tgaaaccacg ttgagctatt tcagccagat ggctggctta acggtgcaaa actttctttc
     1081 tgccgccagc gggattgcgg tgatttttgc cctcatccgt gcgtttaccc gccagagcat
     1141 gagcacactc gggaatgcct gggtcgatct gctacgcatc acgttatggg tgctcgtccc
     1201 tgtggcgttg ttgatcgcac tgttttttat tcaacaaggt gcgctgcaaa actttcagcc
     1261 ttatcaggct gtgaataccg ttgaaggagc gcaacagctg ttacccatgg ggcctgtagc
     1321 ttctcaggaa gcgatcaaga tgctcggtac taacggcggt ggcttcttta atgccaactc
     1381 gtcgcatccg tttgaaaacc caaccgcact gaccaacttc gtgcagatgc tggcgatctt
     1441 cttgatccca acggcgctgt gctttgcctt tggtgaagtg acgggcgatc gccgccaggg
     1501 gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc tgcgtaggcg tggtgatgtg
     1561 ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc gcagacagca gcatcaatat
     1621 ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg tcgtgactac
     1681 ggcggcttcc tgtggcgcgg tgattgcgat gcatgattcg tttaccgctc tcggtggcat
     1741 ggtgccgatg tggctgatgc aaattggtga agtggtgttc ggcagtgtcg gttctggtct
     1801 ttacggcatg atgctgtttg tcctgctggc ggtgtttatt gccgggctga tgattggtcg
     1861 tacaccggaa tatctgggta aaaaaatcga cgtacgcgag atgaaactga ctgcactggc
     1921 gattctggtt accccgacgc tggtgctgat gggcgcggcg ctggcaatga tgaccgacgc
     1981 cggacgtagc gccatgctca accctggccc gcatggtttt agcgaagtgc tgtacgccgt
     2041 gtcatccgcc gctaacaaca acggcagcgc ctttgccgga ttaagcgcca actccccgtt
     2101 ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc ttcggggtga ttatcccggt
     2161 gatggcaatt gccggttcgc tggtgagtaa aaagagccaa cccgccagct ccggcacgct
     2221 gccaacgcac ggcccgctgt ttgttggcct gttaatcggc accgtgttgc tggttggcgc
     2281 actgaccttt atccctgccc tggcgcttgg tccggtggcg gaatatctct cctgatgata
     2341 ttgagtgagc actgaatatg agtcgtaaac aactggcgct attcgaacca acacttgtcg
     2401 ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca ggcgcaatgg cgcaatccgg
     2461 tgatgtttat cgtctggatc ggcagtctgc tgaccacctg tattagcatc gcgatggcaa
     2521 gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat tagcggctgg ctgtgggtca
     2581 ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg ccgcagtaaa gcgcaggcca
     2641 acagtcttaa aggggtgaaa aaaactgcct ttgcccgcaa gctgcgtgag ccgaaatatg
     2701 gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa aggtgacatc gtgctggtag
     2761 aagctggcga tattatcccc tgcgatggtg aagtcattga aggcggcgca tcggtcgatg
     2821 aaagcgccat caccggggaa tcggcaccgg tgatccgtga atccggcggc gattttgcct
     2881 ccgtcaccgg cggcacgcgt attctttctg actggctggt gattgagtgt agcgttaacc
     2941 ccggtgagac atttctggat cggatgatcg cgatggtgga aggcgcacag cgacgcaaaa
     3001 caccgaacga gattgccctg actatcctgc tgattgctct gaccatcgtc tttttactgg
     3061 caaccgccac gctgtggccg ttttccgcga ggggcggtaa tgccgtgagt gtaacggtac
     3121 tggtggcgct gctggtctgt ctgatcccaa ccactattgg cggcctgttg tcagcgatcg
     3181 gcgtcgccgg gatgagtcgg atgctgggcg cgaatgtgat tgccaccagc ggacgtgcag
     3241 ttgaagcggc aggtgacgtt gacgttctgc tactggataa aaccggcacc atcacactcg
     3301 gtaaccgtca ggcgtcggag tttatccctg cgcagggcgt ggatgaaaaa acgctggctg
     3361 acgccgcaca actggcttcg ctggctgatg aaacgccgga aggccgcagt attgtgatcc
     3421 tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca gtcgctccat gccacctttg
     3481 taccgtttac tgcgcaaagc cggatgagcg ggatcaacat cgacaaccgc atgatccgta
     3541 aaggttctgt cgatgccatt cgccgccatg ttgaggctaa cggcggtcac ttccctaccg
     3601 atgttgatca aaaagtcgat caggttgcgc gtcagggggc cacgccgctg gtggtggtgg
     3661 aaggttcccg tgtgctgggc gttattgcgc tgaaagatat cgtcaaaggc ggtattaaag
     3721 agcgcttcgc ccagctgcgc aaaatgggca ttaaaacggt gatgattacc ggcgataacc
     3781 gtctgactgc cgccgccatt gctgcggaag cgggcgtcga tgattttctc gccgaagcga
     3841 caccggaggc caagctggca ttgattcgtc agtatcaggc ggaaggccgt ttggtggcga
     3901 tgaccggcga cggcaccaac gatgctccag cgctggcgca ggcagatgtc gcggtggcga
     3961 tgaactccgg tactcaggcg gcgaaagaag cgggcaatat ggtcgatctc gactctaacc
     4021 cgaccaagtt gatcgaggtg gtacatattg gcaaacagat gctgatgacc cgtggctcgc
     4081 tgaccacctt cagcattgcc aacgatgtgg cgaaatactt cgccattatt ccggcggcgt
     4141 tcgcggcaac gtatccgcag ttaaatgcgc tgaacatcat gcgccttcat tcgcccgact
     4201 ccgccattct cagtgcggtc attttcaacg ccttgattat cgtctttttg attcctctgg
     4261 cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc catgttgcgc cgtaacttat
     4321 ggatttacgg tctgggtggg ctgctggtgc cgtttattgg tatcaaagtc attgatttac
     4381 tgctgaccgt ttgcggtctg gtgtgaggtt taccatgcgt ggcttacgtc cggcattatc
     4441 gacatttatc tttctgttat tgattactgg cggcgtttac ccgctgctga ccaccgtgct
     4501 ggggcaatgg tggtttccct ggcaggccaa cggttcgttg attcgtgaag gtgatacggt
     4561 gcggggttcg gcattaatcg ggcagaattt taccggcaac ggctattttc agggtcgccc
     4621 gtcggcaacg gcagaaatgc cctataatcc acaggcttct ggcgggagca atctggcggt
     4681 cagtaaccct gagctggata aacaaatagc cgcacgcgtt gctgcattac gggccgctaa
     4741 cccggatgcc agtacgaacg ttccggttga actggtgacg gcatcggcaa gcgggctgga
     4801 caataatatc accccgcaag cggcggtctg gcaaatccca cgcgtggcga aagcgcgtaa
     4861 tctcagcgtt gaacagctca cgcaactgat cgcaaaatac agccaacaac cgctggtgaa
     4921 atatatcggc cagccggtcg tcaacattgt tgaactcaat ctggcgctgg ataaacttga
     4981 tgaataacga acccttacgt cccgaccccg atcgtctgct ggaacaaact gccgcgccgc
     5041 atcgggggaa gctgaaagtt ttcttcggtg cctgtgcagg cgtcgggaag acctgggcga
     5101 tgctggcaga agcccagcga ctgcgggcgc aagggctgga tattgtggtt ggcgtggtag
     5161 aaacccatgg gcggaaagat accgccgcca tgcttgaagg gctggctgtt ctgccgccca
     5221 aacgccaggc gtaccgtggg cggcatatca gcgagtttga tctcgatgcc gccctcgccc
     5281 gccgtccggc gctgatctta atggacgaac tggcgcacag taatgcgcca ggttctcgtc
     5341 atcctaaacg ctggcaggat atcgaagaac tgctggaagc tggcattgat gttttcacaa
     5401 ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt cagcggcgtc accggaattc
     5461 aggtacggga aaccgtgccc gatccttttt tcgatgccgc cgacgacgta gtgctggtgg
     5521 acttgccccc ggacgatctg cgccagcggc tgaaagaagg caaagtctat attgccgggc
     5581 aggcggagcg cgccattgaa cattttttcc gcaaaggcaa tctgatcgcc ctgcgtgaac
     5641 tggcactgcg ccgtactgcc gatcgcgtag atgagcaaat gcgcgcctgg cgggggcatc
     5701 ctggtgaaga gaaagtgtgg cacacgcgcg acgcgatcct tttatgcatc gggcataaca
     5761 ccggcagtga gaaactggtc cgcgcggcgg cgcggttggc ctcgcgcctg ggtagcgtct
     5821 ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt accggaaaaa aaacgtcggg
     5881 caattctcag cgccttacgt ctggcgcagg agctgggcgc ggagacggct acacttactg
     5941 atccagcgga agaaaaagca gtagtgcgtt atgcccgtga acataatctc ggcaagatta
     6001 ttctcggtcg cccggcctcg tgccgctggt ggcgtcggga aacgtttgct gaccgactgg
     6061 cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct tgatgaacca cccgcccgca
     6121 cgattaacaa cgcgccggat agccgctctt ttaaagacaa gtggcgggta cagattcagg
     6181 gatgcgtggt tgccgccgcg ttatgcgccg ttatcacttt aattgccatg cagtggctga
     6241 tggcgtttga tgccgccaac ctggtgatgc tgtatctgct tggcgtggtg gtggtggcgc
     6301 tattttatgg acgctggcct tcggtggttg ctaccgtcat taatgtggtg agtttcgatc
     6361 tcttttttat cgccccacgc ggcacgctcg ctgtctctga tgtgcaatat ctgctgacct
     6421 tcgcggtgat gttaaccgtc gggctggtga tcgggaacct tactgcgggt gtgcgttatc
     6481 aggcgcgggt agcccgttac cgcgagcaac gcacacggca cttatatgaa atgtcgaaag
     6541 ctctggcggt gggccgcagt ccgcaggaca tcgccgccac cagcgaacaa tttattgcct
     6601 ccacgtttca tgcccgcagt caggtgttgt tgcccgatga caacggtaaa ttgcagccgt
     6661 taacacatcc gcaaggaatg acgccatggg acgatgccat cgcgcagtgg agttttgata
     6721 aaggcctgcc tgcgggtgcg ggcaccgaca cgttacccgg cgtgccgtac cagattttgc
     6781 cgctaaaaag cggcgagaaa acctacggac tggtggtggt ggaaccgggg aatctgcgtc
     6841 agttgatgat cccggagcag cagcgcctgc tagagacgtt tacgctgtta gtcgccaatg
     6901 cgtttgagcg gctgacgctg accgccagtg aagaacaggc gcggatggca agcgaacgtg
     6961 aacagatccg caacgccctg ctggcggcgc tttcgcatga tttacgcacg ccgcttacgg
     7021 tgctgtttgg tcaggcagaa atcttaacgc tcgatctggc aagcgaagga tcgccccacg
     7081 cccgccaggc cagcgagatt cgtcagcatg tgctgaacac tacccgactg gtgaataatc
     7141 tactggatat ggcgcgaatt cagtccggcg gctttaattt gaagaaagag tggttaacgc
     7201 tggaagaagt agtcggcagc gctctgcaaa tgctggaacc gggtttatcg tcgcccatca
     7261 atctttctct gccagaaccg ctgaccttaa tccacgttga cgggccactc tttgaacggg
     7321 tgctgattaa tctgctggag aacgcggtga aatatgcggg tgcgcaggcc gaaattggta
     7381 tcgatgccca cgttgagggc gaaaatctac aactggatgt ctgggataac ggccccggtc
     7441 ttccgccagg ccaggagcag acgatatttg ataagtttgc tcgcgggaat aaagagtcgg
     7501 cagtaccggg ggtagggctt ggactggcaa tttgtcgggc gatagtggat gtacacgggg
     7561 gcactattac cgcgttcaac cgaccggaag gtggtgcctg ttttcgtgtt acacttcccc
     7621 agcaaaccgc tcctgaactt gaagactttc atgaggatat gtgacaaacg ttctgattgt
     7681 tgaagatgaa caggctattc gtcgctttct gcgcacggcg ctggagggcg acgggatgcg
     7741 cgtctatgag gccgaaacgc tgcaacgcgg cttgctggaa gcggctaccc gtaagccaga
     7801 tttgattatt ctcgatctcg gcctgcccga tggtgatggg attgagttta tccgcgacct
     7861 gcgccagtgg agcgcggtgc cggtgattgt gctttccgca cgcagcgaag agagcgacaa
     7921 aatcgccgcg ctggatgccg gagcggatga ttatctgagt aagccgtttg gcattggcga
     7981 attgcaggcc cgtctgcgcg tcgctttacg ccgccactcc gccaccgccg cgcccgatcc
     8041 gctggtgaaa ttttccgatg ttaccgtcga tttagccgcc cgcgtgattc accggggtga
     8101 ggaagaggtg catctcacgc cgattgagtt ccgcctgctg gcggtgctgc taaataatgc
     8161 cgggaaagtg ctcacccagc gccagttact caatcaggtg tgggggccaa acgcggtcga
     8221 acacagtcac tatttgcgta tttatatggg acatctgcga caaaaactgg aacaggatcc
     8281 cgctcgcccc cgccatttca ttactgaaac cggtattggg tatcggttta tgccatgaat
     8341 gtttattcaa taaatgcgct taaataatta ttaatttatt attaaacctg ccaaaaatat
     8401 tattatttgg caggtttaat ttcttaacgc aaatataaaa cacaaaatta caacatttaa
     8461 ataaca
//
LOCUS       DAZFJF010000167         8443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFJF010000167 DAZFJF010000000
VERSION     DAZFJF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.168"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..292)
                     /locus_tag="ACHKAU_004407"
     CDS             complement(<1..292)
                     /locus_tag="ACHKAU_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO5533344.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAAC"
     gene            complement(289..1959)
                     /gene="menD"
                     /locus_tag="ACHKAU_004408"
     CDS             complement(289..1959)
                     /gene="menD"
                     /locus_tag="ACHKAU_004408"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO5533345.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2048..3343)
                     /gene="menF"
                     /locus_tag="ACHKAU_004409"
     CDS             complement(2048..3343)
                     /gene="menF"
                     /locus_tag="ACHKAU_004409"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO5533346.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3422..3727)
                     /gene="elaB"
                     /locus_tag="ACHKAU_004410"
     CDS             complement(3422..3727)
                     /gene="elaB"
                     /locus_tag="ACHKAU_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO5533347.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3782..4243)
                     /locus_tag="ACHKAU_004411"
     CDS             complement(3782..4243)
                     /locus_tag="ACHKAU_004411"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5533348.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4308..5225
                     /gene="rbn"
                     /locus_tag="ACHKAU_004412"
     CDS             4308..5225
                     /gene="rbn"
                     /locus_tag="ACHKAU_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO5533349.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5413..6632
                     /gene="elaD"
                     /locus_tag="ACHKAU_004413"
                     /pseudo
     CDS             5413..6632
                     /gene="elaD"
                     /locus_tag="ACHKAU_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
     gene            complement(6703..8442)
                     /locus_tag="ACHKAU_004414"
     CDS             complement(6703..8442)
                     /locus_tag="ACHKAU_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO5533350.1"
                     /translation="MIMLLMSSLILSGCGPESENKESQQQQPSTPTDQQVLAAQQAAI
                     KEAEQRSVADKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQIA
                     NPGTARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEEI
                     VNYFPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELPA
                     SNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGS
                     HKAEINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDP
                     KSIESMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNS
                     EMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHI
                     TLLFELTLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLG
                     PTINAPSEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIR
                     LIELADGVTDISQ"
BASE COUNT         2135 a         2199 c         1955 g         2154 t
ORIGIN      
        1 ggcaagccgc catcatcgcc acccgtccgc caagcgagta ccccaccagc cagaagtcaa
       61 ggatgttgta actaaccaag gttttacgca gtaagtcggt gacatcatca aatccatcga
      121 cgctaatcgc cgccgaacca ccatgacctg ggagatcaac atacaaccgt gagtagtcgg
      181 caaacgcctc gcccacttct tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca
      241 ccagccaggg taaacctggt tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct
      301 tacctgcgcc agaagttgct ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc
      361 aatcaccgtg gtggttggcg tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca
      421 gttttgcgga cgatgatatt tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt
      481 ttgcggcatc agatagaaac gctcacgctc gctttgcggc gttggcaaca gcgagaaaat
      541 ttgcccgccg ttgttgttca ccacaattaa taccagcggc gcagaaacct gacgcaataa
      601 cgccagcgcg ttgagatcgt aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt
      661 gccgcttgcc cgctgaacgc ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc
      721 tcggttgctg tacaccgggt aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac
      781 caccaggctg ttaccaacaa acaattgccc ctgttcaggc agatagtcgc tgatgcgatg
      841 cgccagttgc gcttcgccaa acgcatcacg gcgggcaata accgcctgca ttgcctgttc
      901 cgccaggcgc gggatttcaa cgcaccaggg ctggcgtttt tctgccggat gcagctccag
      961 ccagtcggca atattggcaa ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc
     1021 ttcaatgtca tcaacaatcc agtactcttc gggttcacag cttgcctgcc attgcaggag
     1081 ccgtttcccc gtcaggctgc ttcccagttg caccacaatt tgcgcctgct gcagctcgct
     1141 ggtcgctttg gcattgccta accaaagatc ggcacacggc agcggctgcc cggtttgtga
     1201 cagcacatcg ccaatcagcg gccagccaag agtttgcgcc cacagggcaa cttttttgcc
     1261 ctcttccgca ctcatgcgcc cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca
     1321 gtcgcgctgt ttttcacttt ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg
     1381 ccaccagtca cccagacgct gttgccagct aagcccggta tcgtccattt cgccatacag
     1441 cggttcagca aacgggcagt tgatatggac tccccccgca tgaagcgtac cgagagcgtg
     1501 gtcgatggta gaaaccagcc aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat
     1561 actgtgcgtg gggtgagagg cgaacattcc cggctggcga attgcctgat tcgcgccgca
     1621 gtcaattagc tccggcgggc gatcggcggt taagagaatc agtttttctc cggttaaccc
     1681 ggcttcaatc agtgccggat agagatttgc caccgccgtg ccggaggtca caatcaccgc
     1741 caccggctgc ttgctgactt tcgccagccc cagcgccaga tgccccagtc cacgctcatc
     1801 gaaatgggtg tggtgaatga atgcggaatt ctccgccgcc gctaacgtta acggtgtaga
     1861 acgcgagcct ggggcgatac agatgtgtct gacgccgtga cgcgttaatg cttccagaat
     1921 gaccgccgcc cagcgtcggt taaatgcgct tactgacatg agtttgtccg gtatcaatat
     1981 tgcggctaag tataaggagc tacaaaaata gattattgat atgaatcggt aatgatgcga
     2041 ctcattacta ttccatttgt aataaagtac gcagccctgc cgctttgttg tcgatttcct
     2101 gccactcttg ctcggggtcg gaaccacgga caatgcccgc gccagcatat agccgcacga
     2161 cattgccgct gatttttgct gaacgcaggg aaacgcagaa ttcgctttgt tgtaatgaga
     2221 gatagcccgc agaaccggcg taccattctc gggtgaacgg ttcgtgacgg gcgataaact
     2281 gtcgcgccag atcgcgcggt aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac
     2341 agatcacatc atccgctttg ttgagtgaag tccagataca gcggcgaaga tgctgcactt
     2401 tacgcagacg cagtacctgc ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc
     2461 gttggcagat atcttccacc accagcatgt tctcgcgctg gtttttatca tccgccatca
     2521 gccactctcc taactgctgc gcctgcttat catcaggatt atttgctact gttcccgcca
     2581 gcgcttcagt acgcagcgct ttgtcacgcc gccgccataa ccgttccggt gaagagccaa
     2641 gaaaagcatt ttcgccatca aaggccatgt aaaaatggta gcaattcaga ttcagtcgac
     2701 gactggcagc catcatcgcc gccgcgttga ccggacttgc gaaatgcagg tcagttgccc
     2761 gagcgagcac cactttgtcg agctcacctt cggcgatggt tttcgttgcc agttcgatta
     2821 attgcgtcca gcccgttttg tccggccagt gttgttctcg cgtggtggtt aaatgtaacc
     2881 caggcaaggg cttgatactc accagtgtgg cgataaattc ttttgcctga atcgcatcgt
     2941 gctgaaggga gctttcgctg aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc
     3001 gccattccag gcggggtaaa agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc
     3061 aaatgcgtaa gtcggcgtgt tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg
     3121 acgtaaaacg ggtaatcgcg cccaggacgg ctgcttcttc atcaccatta cgttgttgcc
     3181 agtagaattg cgggtacgtt tgctgactgg ccagccagct caaggcatca aaagcgtctt
     3241 tgagagggaa aggaatatcg ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca
     3301 aatggcgcag tagattttcc agcgccgtag taagtgattg cacgcggacc tctccctgtt
     3361 aaaagcctca cattatacgg ggtactacaa aaaaaatgca gtaccccggt gtagggaggt
     3421 tttaacggcg tgccagcaac agtcctagta ccagcccaac ggccgcgccc acaccaattc
     3481 cttgccaggg tttttcgtgg acgtagtcat cagcacgata aactgcctgc ttcgcccgat
     3541 agtaataact gtctgaagcc tggctaaccc gttttttcac atcatccagc gctttttctg
     3601 cacgcgcttt cagctcaaca tatttctgat cggcgggatc gccagaggag cggagcacct
     3661 cttccagtgt ttcactaagc agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat
     3721 tagacattct cgttctccat ttgcgtaaaa cctgtcacta actatagaca agggttgttg
     3781 attacgcctg aattacctcg cgtgccatcc caatgtgtgg gataccatct tcttcataga
     3841 cctccgtcac agggataaaa ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc
     3901 cgaggtaaac aggcttatca ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca
     3961 gctgctgccc cactttttcg ccacgcaacg cttcactgac aatcacccga cctataacga
     4021 ccggctcaag atcatcatca cttttcagaa tcctcgcata tgccaccagt tcatcatttt
     4081 tccaaccgag aatatggcga ttatcccccg tcaaatcatc accatcgata tcctgataag
     4141 ggcaattctg ctcgacaaca aacaccgcgc aacgcagttg taataaggca taaagctggg
     4201 aaaccgataa ttcagagtga tgcagatctt gccattcaat cataatgctc tccttttaga
     4261 tgtatgcctt atactagccg gaatattttt tgaaacgtga tgaactcatg gaattaattt
     4321 ttttaggtac ttcggccggt gtgccaaccc gcacgcgcaa tgtcacggca atattgctta
     4381 acctgcaaca tccgacccag agcggtcttt ggttgtttga ctgcggtgaa ggcacccagc
     4441 atcagctact gcataccgcc tttaaccctg gaaaactgga caagattttt atcagtcacc
     4501 ttcatggcga tcatcttttt ggtttacccg gcttgctgtg cagtcgttct atgtcaggca
     4561 ttatccaacc cttaacgatt tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc
     4621 tgcggattag cggctcatgg accgattatc cgctggaaat tgtcgaaatt ggcgctggcg
     4681 aaattctcga tgatggcctg cgcaaagtaa ccgcttatcc gctggaacac ccactggaat
     4741 gttatggcta tcgtattgaa gaacatgata aaccgggtgc attaaatgcc caggcattaa
     4801 aagctgctgg cgtgccgcct ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca
     4861 cgctggaaga tggaaggcag attaacggcg cagattactt agctgctcca gtgccaggta
     4921 aagcgctcgc tattttcggc gataccggcc cctgcgatgc cgcacttgac ctggctaaag
     4981 gtgtcgatgt catggtgcac gaagcgacgc tggatataac catggaagcc aaagccaata
     5041 gtcgcggcca tagctctaca cgccaggctg cgacactagc ccgtgaggct ggagtcggta
     5101 agctaatcat tacccacgtc agctcgcgct atgatgacaa aggttgtcag cacctgttac
     5161 gtgaatgcag gtcaattttc ccggcgactg aactagcgaa tgatttcacc gtgtttaacg
     5221 tttaactctg cttattatta aaaacagggc gaaacttgcc ctgttatcgc aacccgcgct
     5281 acatatatcc acccacacgg gcatattgag cgatttttga tatttattca tctccttacg
     5341 cactatattc agagcaataa gttgcctctt tttatcttca ttcagcacaa cctttgcaac
     5401 taaaaggagc ccatgatggt tacagttgtc agcaattatt gtcaattatc tcaaacccaa
     5461 ctcagtcaga catttgcaga aaaatttact gtgaccgagg aattactgca gtctttaaaa
     5521 aaacagcgtt atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt
     5581 atgatgaatt tggaaaaaaa gctgaagata ttctttacca tattgttaga aacccaacaa
     5641 atgagaccct atcgattatc cgacttatta aaaatgcctg tttaaaatta tataatttgg
     5701 cacataccgc aaccaactcc caccttaaac caactggtcc ggataattca gatgttctcc
     5761 tgttcaaaaa gctattctcc ccttcgaaat taatgacaat tatcggtgat gaaattcctc
     5821 ttatatctga aaaacagtcg ctttcaaagg tgcttttaaa tgatgagaat aatgaactga
     5881 gtgatgatac aaacttctgg gataaaaatc gtcaattaac cacagatgaa atagcttgct
     5941 atcttcagaa gatcgccgct aatgcaaaaa atactcaagt caattatcct actggtctct
     6001 acgtccccta ctccaccaga actcacctgg aagacgctct caatgaaaat attaagagcg
     6061 atccatcatg gccgaatgaa gtccagttat tccacataaa tactggcgga cactggatat
     6121 tagtttcgct acagaaaata gtaaataaaa aaaataataa actacaaata aaatgcgtca
     6181 tattcaactc attgcgtgca ctaggccatg ataaagaaaa ttcacttaag cgtgtcatta
     6241 atagttttaa ttctgaactc atgggagaaa tgtcgaataa taatataaaa gttcatttaa
     6301 atgaaccaga gataatattt ttacatgccg atcttcagca atacttaagc caaagttgcg
     6361 gtgcgtttgt gtgcatggca gcccaggaag tgattgaaca aagggaaagc aattctgaca
     6421 gcgcccccta tacgttatta aaaaactatg ctgacagatt taaaaaatat tcagcagaag
     6481 agcagtacga aattgatttt caacatcgac tggtaaacag aaattgttat ttagataaat
     6541 atggcgatgc acgaatcaat gcttcttata cacaattaga aataaaacac tcacaaccca
     6601 aaaatagagc atccggcaaa agagtgagtt aacatcattg ttaatcaatt cgaccagtca
     6661 gcaaatatgc tgactggtcg aacatagttt gctaaacagt catcactgac tgatgtcagt
     6721 cacaccatcc gccagttcaa tcaggcgaat aaattccgcc ctgtaaccct gtggatcttc
     6781 cccttttgcc tgctgagccc actgtttgat ctgctgccag gaggtattgt tcaggtattc
     6841 agaaccgcgt aacttttgcc catatgcagc tactgctgcg caaaaacgca tatcttcaga
     6901 gggggcgttt attgttggcc ccagcgggaa ttcaactaac tgactttctt ttccctgcgg
     6961 gtatttccag cgaatcttta accaggccag ttctttcgtt ttgtccgatt tcactgattt
     7021 gttatccggg gcatagcgta acttatcaat tgatgctttt tgcccgttca gcgttaattc
     7081 gaataacaac gttatatgtt tgcctgcgcc aatatccccc gcatcaacgt tgtcgttatt
     7141 aaaatcttcc gcccgaagtt ggcgcttttc ataaccaatc tgacggtatt ccgttaccca
     7201 cgcggggtta aactcaattt gcgctttgac atcttttgct acggtaatca acgtctgccg
     7261 catttcacta ttcaatactt tctgcgcttc agagagggta tcaatgtagc tgtagttgcc
     7321 gttaccaaca tcggcaattc gcaccatcat tgcctcgttg taattgctat tccccacgcc
     7381 aaacgtcgac agagtaacac cagactcccg ctgttttttg accattgatt caatcgattt
     7441 tggatcgtca atgccaacgt taaagtcacc gtcagtggct aataaaatgc gattgatgcc
     7501 gcccttaata aaccccttcg ccgcctgctg ataagccagt tccagcccgg caccgccatt
     7561 ggtactgcct tcggcatcca gcgaatcaat tgcggcatta atttccgcct tgtgactacc
     7621 ggagatagaa ggcaacgcaa tacgggagtc gccagcgtag gtcacgatgg caatgttatc
     7681 ctgctcacga agttctttga ccaataattt caacgaagac tggataagtg gcaaacgttc
     7741 atcagaaatc attgaaccag aagtgtcgat aagaaagacc agattagaag ctggtaactc
     7801 ttcactttta tgatctttcg ccaggatatc aattttcagc aatgttagct gttcattcca
     7861 cggcgcaggt gccagttcgt agcgcatagc gaaaggtatt ggcttagtgg ccggaataga
     7921 ttgtttgtct ttgatatccc agtcatacgg gaaataattg actatctcct ccacccgcac
     7981 agcgtctggc ggaggtaaca gcccatgatt gagaaaacgg cgtacattcg cataactgcc
     8041 ggtgtcgacg tcaaggctaa acgtcgccaa cgggttttgc gctacctgct taaccggatt
     8101 atcatcgaac tgcttgtagc gagcggttcc tggatttgcg atttgggacg ctgctttttc
     8161 tctggctgca tgttgatatt ttggagccgc ctgtaagcgc ccctgcaaag cctgtttgtc
     8221 tgaatattgt tgcgcttctt gctgggctaa ggcttttgct ttcgcgtctg cagtggcttt
     8281 atcggcgacg gatctttgct cggcctcttt tattgcagct tgttgcgcgg caagcacttg
     8341 ctggtctgtg ggggtactgg gctgttgttg ctgactttcc ttattctcag attccggccc
     8401 gcatcctgac aaaatcaaac tactcataag caacatgatt ata
//
LOCUS       DAZFJF010000168         8384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFJF010000168 DAZFJF010000000
VERSION     DAZFJF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.169"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(399..1592)
                     /gene="tyrB"
                     /locus_tag="ACHKAU_004415"
     CDS             complement(399..1592)
                     /gene="tyrB"
                     /locus_tag="ACHKAU_004415"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO5533351.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1827..2924)
                     /gene="alr"
                     /locus_tag="ACHKAU_004416"
     CDS             complement(1827..2924)
                     /gene="alr"
                     /locus_tag="ACHKAU_004416"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO5533352.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            complement(2977..4392)
                     /gene="dnaB"
                     /locus_tag="ACHKAU_004417"
     CDS             complement(2977..4392)
                     /gene="dnaB"
                     /locus_tag="ACHKAU_004417"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5533353.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4475..5458
                     /locus_tag="ACHKAU_004418"
     CDS             4475..5458
                     /locus_tag="ACHKAU_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO5533354.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTRERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5624..5866)
                     /gene="pspG"
                     /locus_tag="ACHKAU_004419"
     CDS             complement(5624..5866)
                     /gene="pspG"
                     /locus_tag="ACHKAU_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO5533355.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(6000..7037)
                     /gene="dusA"
                     /locus_tag="ACHKAU_004420"
     CDS             complement(6000..7037)
                     /gene="dusA"
                     /locus_tag="ACHKAU_004420"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO5533356.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7400..8107)
                     /locus_tag="ACHKAU_004421"
     CDS             complement(7400..8107)
                     /locus_tag="ACHKAU_004421"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO5533357.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNLGDNK
                     DAMPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(8131..8382)
                     /gene="yjbL"
                     /locus_tag="ACHKAU_004422"
     CDS             complement(8131..8382)
                     /gene="yjbL"
                     /locus_tag="ACHKAU_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO5533358.1"
                     /translation="MKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNIY
                     GKSIDYAALRYRDIIIAKIDLFIQRITHNLWDARKKMCF"
BASE COUNT         2221 a         2126 c         1944 g         2093 t
ORIGIN      
        1 gatattggta atcaatataa ggttaaactt acacaaagcg gaaaaaaaga gctctcttta
       61 actttagaat ttaacgatac tataattgaa tctcagaaag tcacaggcaa taaggcaaag
      121 catattcttg aggatataga aatattctat cgtaacaaac ccgatactta ttattaaatt
      181 taataatggt tagatgtgca gaaattatta ctccacccaa aaaaacagga taaggaattc
      241 cagataattg ggataagctt aacaataaaa gaactaacaa cgtttattat ctctcacgta
      301 gaacgatggc atcaaaaaaa tcatctataa agaaagagtg aaagcgacga cagtttaatt
      361 tcactgcagg ctgggtagct ccagcctgct ttcctgcatt acatcaccgc agcaaacgcc
      421 tttgccacac gttgtacatt tgccgtattt aacccggcga cacacatgcg accgctggcg
      481 atgagataga caccaaattc ttcacgtagt cggtcaacct gagcggcact taaaccggta
      541 taactgaaca tgccgcgctg attaagcaga taatcgaaat tgcgttctgg catctctgtg
      601 cttaatacct tcaccagttc ctgacgcatt gccagaatgc gagtacgcat ctcttctact
      661 tccgccagcc agctggcttt caatgcctcg tcattcagca ctgcagccac cacctgcgca
      721 ccaaaattcg gcgggctgga gtagttgcgg cgaactgttg ctttcaattg ccccagtacg
      781 cggccagcgg cttcggcatc ttcacacaga acagaaagtc cgccgacgcg ctcgccgtaa
      841 agggagaaaa ttttcgagaa cgaattgctc accagagcgg gtaatccagc gctggcaatg
      901 gcgcgaatag cgtaggcatc ctcttccata ccggcaccaa atccttgata ggcaatatcg
      961 aggaatggaa taagctcgcg ggatttgaga atttcaatca ccgcatccca ctgatcatta
     1021 gtgagatcgg cacccgttgg gttgtggcaa catggatgca gcaacacaat actgcgggca
     1081 ggtaatgttt tcagcgtcgc caacaggtca ttaaagcgca cgccgttagt cgcttcgtca
     1141 taccaggggt aagtactcac ttcgaatcca gccccggcga atattgctac gtggttttcc
     1201 caggtaggat cgctgaccca gacgcctgat tccgggaagt agcgtttcag gaaatccgcg
     1261 cccactttca atgcccctga gccgccaagg gtttgaatgg ttgctacgcg ctgttgttgc
     1321 agtactggat ggtccgcacc aaacagcagc ggcgcaatgg catggcgata gctgttaagc
     1381 ccttccatcg gtaaataaag cgaagcgcca tgaggctgcg cattcaggcg cgcttccgcc
     1441 tccgccacgg cttgcagttg tggaataatt ccgtcttcgt tgtagtacag accgatactt
     1501 aaattcactt tgtcgctgcg agggtcttct ttaaaacgct ccataagcgt aagaatcggg
     1561 tcgccagcgt aggcgtcaac tttttgaaac acgcgatggt tctccaggtt tacgggcagg
     1621 tggttaaaac acaataaacc ggaagaaggc gaagatcgag tggatgttca ggagcgaacg
     1681 gcaattagca acagagtgag actcatgaca aacgtacatc cgccagaggc acgaccttta
     1741 taagagcatg aaagaacatc aacttattga atttttagga ttttattggc cggataaggc
     1801 attcacgccg catccggcac agacaatcaa atattacaga acgataaatc cacgtatttc
     1861 atcgcgaccc ttgaagtcag gcgcgtaata agttcgtaag cgcttacttt cgtcatttca
     1921 gcgatacgtt ctacgggcaa accttcgccc cataaaatga ccggatcccc ggctttgtcc
     1981 tgcgcttgtg gacctaagtc tacgcagatc atatccatcg ccacgcgccc gacaatcggt
     2041 acttcgcgac cgttcaccag cactggcgta ccggacggcg cggcgcgcgg ataaccatcg
     2101 ccatagccca tcgcgactac gccaagacgg gtatcacgtt cgcttaccca ggttccacca
     2161 taaccaacag gttctccggc tttatgctca cgcacggcaa tcaggctgga ggttagtgac
     2221 atcactggct gacagccaaa atcggcaccg gtggagcgat cttccagcgg cgagacgcca
     2281 taaagaatga tgcccgggcg cacccagtca aaatgcgact gtggccacag cagaatgcca
     2341 cccgatgcgg caatggaacg ttgaccaggt ttgccttcgc aaaaggtatt aaagatagcg
     2401 agttgtttct cggttgcgcc acattttggt tcatccgcgc gcgcaaaatg gctgacgata
     2461 ttcaccggct gacgaacgtt tttgcactgg gtcaggcgat gataaaacgc ctcagcctgt
     2521 tccggcctta cgcccagacg gtgcataccg gtatcgagtt tcatccagac ggtaaccggc
     2581 tcgtccaggc tagcctcttc cagcgcagcc agctgttctt cgttatgcac ggcggtatga
     2641 aaatgttgcg cagaaatcgt cggcagatct ctggcatcaa aaaagccttc gagtaacagt
     2701 acaggtttgg tgattccccc cgcacgcagt cgcagagctt cttcgagacg ggctacgcca
     2761 aaggcgtcag catcggggag cgttcgcgcg gtctcaagaa gaccgtgacc ataagcgttc
     2821 gctttcacca ccgcaaccat tttactggca ggggccagtt cacgaagacg ttgcaggttg
     2881 tgtcgcagag cgcggcggtt aatcacaaca gttgccgctt gcatttgtgt tccttgataa
     2941 gtgtttgctt taattaccta attcataaaa taattattat tcgtcgtcgt actgcggccc
     3001 cgcatagttg tcgaagcgcg accattgacc gttaaaggtc aggcgtaccg tcccgattgg
     3061 gccgttacgt tgtttaccga taataatttc cgcgatgcct tttaaatcac tgttttcgtg
     3121 atacacctca tcacgataga taaacatgat caagtccgca tcctgctcga tagagccaga
     3181 ttcacgcagg tcggagttga ccgggcgttt gtcggcacgt tgttccagag aacggttcaa
     3241 ctgggacagc gccaccaccg gcacgttcag ttctttcgcc agtgctttca gcgagcggga
     3301 gatttctgca atttccagcg tacggttatc ggaaagcgcc ggtacgcgca tcagttgcag
     3361 gtagtcgatc atgataagcc cgatgccgcc gtgttcacgg gcaatacggc gtgcgcggga
     3421 acgcacttcc gttggcgtca ggccggagga gtcatcgata tagatattgc gtttttcgag
     3481 caaaataccc atggtgccgg aaatgcgcgc ccagtcttca tcatcgagct gcccggtacg
     3541 gattttagtc tggtcaacgc gcgacagcga cgccagagaa cgcatcataa tctgttctga
     3601 aggcatctcc agcgagaaga taagtactgg tttatcctgc aacatcgccg cgttttcgac
     3661 gaggttcatc gcaaatgttg ttttacccat cgacggacgc gcggcgacga tgatcaaatc
     3721 cgacggctgc aagccagcgg tttttttgtt gagatcgtca taaccggtgt ttactccggt
     3781 aacgccatcg tgtggctgct gaaacaactg ctcaatacgc gccacggttg cgtcgagcac
     3841 atcggcgatg ttcttcggcc cttcgtcttt gtttgcacga ctttcggcaa ttttaaagac
     3901 gcgggattca gcaaggtcca gcagatcttc gctggtacgc ccctgcggat caaaaccggc
     3961 ttcagcaatc tcattcgcaa ccgagatcat ctcacggaca acggcacgtt cacgcacgat
     4021 gtcagcataa gcactgatgt tcgccgcact tggcgtattt tttgacagct ctgccagata
     4081 agcaaaacca ccgacgctat cgagttgccc ctggcgttcc agcgattccg caagggtaat
     4141 cagatcgata ggactaccgc tttcctgcaa acgcgccatt tcagtaaaga tatggcggtg
     4201 tgggcgggtg taaaaatcgt ctgccaccac acgctcggct acatcgtccc agcgttcgtt
     4261 atccagcatt aaaccgccca acaccgactg ctccgcttcg atcgagtgcg gaggcacttt
     4321 cagcccggca acttgtggat cgcgttcgcg gggttcagcc tgctgtttgt tgaagggttt
     4381 atttcctgcc atagtgaatg gagttaccga gataaagaat gggtcgaaac tttaccatat
     4441 gaagcagccc ctgacgatac gttctggagg acacatggca acacgaattg aatttcacaa
     4501 gcacggtggc ccggaagtac ttcaagccgt agagttcact cctgccgatc cggcagagaa
     4561 tgaaatccag gtcgaaaata aagccatcgg catcaatttt atcgacacgt atatccgcag
     4621 cggcctttac ccgccgccat cactacccag cggattaggt accgaagcgg caggcatcgt
     4681 gagtaaagtc ggcagtggtg taaagcatat taaggcaggc gatcgtgttg tctatgcgca
     4741 gtcggcgtta ggcgcttaca gctctgtgca taacattaat gcggataaag cggcgattct
     4801 gcctgcggca atttcttttg agcaagctgc ggcatccttc ctgaaaggct taacggttta
     4861 ttatctgctg cgtaaaacct atgaaattaa acccgatgaa cagttcctgt tccacgcagc
     4921 ggctggcggc gttggcttga ttgcctgcca gtgggcaaaa gccctgggcg cgaaacttat
     4981 cggcaccgta ggaaccgcgc aaaaagcgca gagcgcgcta aaagcgggcg cgtggcaggt
     5041 aattaactat cgtgaagaga atctggtcga gcggttaaaa gagatcaccg gcggtaagaa
     5101 agtgcgcgtg gtgtacgatt ccgtgggcag agacaccagg gaacggtcgc tggattgcct
     5161 gcaacgccgc ggcttaatgg tcagttttgg caactcatca ggtgcggtta ccggtgtgaa
     5221 cttaggcatt ctcaatcaaa aaggctcgtt gtatgtgaca cgcccttccc tgcaaggcta
     5281 tatcaccacg cgggaggaat taaccgaggc cagtaatgaa ctgttctctt tgattgccag
     5341 cggtgtgatt aaggtcgatg tcgccgagca gcagaaatat ccgctgaagg atgcgcagcg
     5401 tgcgcatgag attctggaaa gccgggcgac gcaaggttcc agcctgctga ttccataaaa
     5461 gaaatagggc ttccacctgg gaagcccttt ctttttatag ttcggctgta tgtagggtac
     5521 agcacgatga atctgttaga ggcgcaatag tgacagattt gattatcaat tcctattttg
     5581 ttctaaggat aaaaccttag gttgtgatca tccgcacaat cccttagtaa cgccagcggt
     5641 cataacgctg atatttaggc acttttggtg ctttaatcgc cttaataacc cacaccaccg
     5701 caatcgccag tagtaaccac ggcagcaact taatcatcaa tgccagcata ccgccgagga
     5761 acataatggc cgtcgccaca accagcgcgg cgataatgcc cagcaacgaa acgccggtga
     5821 ccatcagcat gacaaaaaag ccaatcacaa aaagtagttc cagcatgatg ctctcccaaa
     5881 tatgaaatct cttgctggca ttacaagaat catgccaaaa ataatctatt gatttaacag
     5941 caaaacgccc cgcgacggtg cgcagggcgt ggtgaatttg actacttttt ggtgaaaagt
     6001 taacgcttat ccgccaccag tttgagcgcg tgttccagca cattaatgtc tgcacccgct
     6061 ttatgggcat tttcacttaa ataacgccgc cactgccgcg cgccaggaat accctggaac
     6121 aagcccaaca tatgccgggt aatatggccg agatacgtcc cctggctgag ttcacgctca
     6181 atgtacggat acatggcgcg cactaccgcc accggatcgg catcggtatc cgaggaacca
     6241 aagatctccc ggtctaccgc cgccagaata cccggattct gatacgcctc gcgcccgacc
     6301 atcacgccat ccatatgttg caggtgtgct ttggcctctt ccagcgactt gataccaccg
     6361 ttaatcgaca ttgtcagatg cggaaagtca cgcttcagtt gatacacacg cggataatcg
     6421 agcggcggga tttcacggtt ttctttcgga cttaaccccg aaagccaggc tttacgtgcg
     6481 tggatgataa acatctcaca ctcgcctttg ccggaaacgg tgttgatgaa atcgcagaga
     6541 aattcatagc tgtcctggtc atcgatgcca atacgcgttt tcaccgtcac tggaatcgac
     6601 accacatcgc gcatcgcttt cacgcagtcg gcaaccagct gcgtattacc catcagacac
     6661 gcaccaaaca tgccgttctg cacccggtca gacgggcagc cgacattcag gttgatctca
     6721 tcatatccac gcgcttctgc cagcttcgca cactgtgcca gcgccgccgg atcgctaccg
     6781 ccgagttgca acgctaccgg atgttcttct tcactgtacg ccaggtaatc acctttaccg
     6841 tgaataatcg cccctgtggt caccatttcg gtatacagca acgtattgcg ggaaagcaga
     6901 cgcaagaaat agcggcaatg tctgtccgtc cagtcgagca ttggtgcaac gctaaaccga
     6961 ccactccagt gaacgtcagt tttttcaggc attgcgctgg tttggttgat tttttgcatt
     7021 tcagaattac cgtgcatttt caaatgtaga gattatttta tcgatatatc atggggttat
     7081 gttattcagc atcactattc aggaggctca atagcggggt actataccat aacaacagga
     7141 agcgcctgtc tcattgcaaa agaaaattga gatcctctca aggcatgaag ctctcacgaa
     7201 gtgatggaaa taatcttatt agccgttaac tttgttaagg ccaatgataa acaatccagg
     7261 ttcgacgata aataaaaaat cacacattaa actctggtga tatatctccc tgctaatagc
     7321 attgatagag aaaaaagaac ccaataaata ttgggtcctt ttatataatg ccttccatac
     7381 tatcgaagaa cttcacatat tatttctccg atttaacccc gaacaaatca taaattaatt
     7441 tagaagtgtc tgtaagtatt ttaatctctt cctttgaggt tgggtcaaac gacttcgcga
     7501 agctaattaa ttgtggtgca gcatctcgca ttttgcttaa aatatcagga tcgagcgttc
     7561 cttcattcac cagatgtgac atcttatcca gatagccatc aaaattcatt tctcccccat
     7621 cagccagacg cttcatctct cccaggtact tcttcatatc acgttgagat aatttttcaa
     7681 actgagcttt caggtagctc tcattatttt cattaatata cactgtttct gataaatctg
     7741 cgtaggcact agaatatgaa agtgaaatta acagcgaagc aaataatttt aagctgttgt
     7801 catcatgttc ctggcaggca ttaacaaacg ttaaaaaacc agaacctatt gcttgaaaat
     7861 gtatattcgc taaaggttca ttatccttag attcctcata aaatagctga accgtggagg
     7921 gaaggtcatt cttattttct acatcaaaag tacattgttc aatgggcatt gcatctttat
     7981 tatccccaag attacatata ctattaatcg cgaaatgaaa aatagcccgg tcaacaactg
     8041 atgcaataaa gtcacgatcg tttaaatcct catcagtgca atcgtcaata tacttattga
     8101 cccacatttt gagatcattt atttgctcaa ttaaaaacac atttttttac gtgcatccca
     8161 taaattatgc gtgattcgtt gaataaacaa atctattttt gcaataatta tatcccgata
     8221 acgcagcgct gcataatcaa tagatttacc atatatatta tttaatttac ctttgattga
     8281 acctctatta tctaatttat ctttgattgc atcttcatta tcgaattgat ttgaattgag
     8341 tgttttatta aagtatccag ttgcaccggg tattatcttc aaca
//
LOCUS       DAZFJF010000169         8306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFJF010000169 DAZFJF010000000
VERSION     DAZFJF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.170"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            80..931
                     /locus_tag="ACHKAU_004423"
     CDS             80..931
                     /locus_tag="ACHKAU_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO5533359.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            924..1295
                     /locus_tag="ACHKAU_004424"
     CDS             924..1295
                     /locus_tag="ACHKAU_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO5533360.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            1292..2044
                     /gene="fliP"
                     /locus_tag="ACHKAU_004425"
     CDS             1292..2044
                     /gene="fliP"
                     /locus_tag="ACHKAU_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5533361.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENAQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            2047..2319
                     /locus_tag="ACHKAU_004426"
     CDS             2047..2319
                     /locus_tag="ACHKAU_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO5533362.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            2321..3103
                     /gene="fliR"
                     /locus_tag="ACHKAU_004427"
     CDS             2321..3103
                     /gene="fliR"
                     /locus_tag="ACHKAU_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5533363.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            3093..4232
                     /gene="flhB"
                     /locus_tag="ACHKAU_004428"
     CDS             3093..4232
                     /gene="flhB"
                     /locus_tag="ACHKAU_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFO5533364.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            4216..6309
                     /gene="flhA"
                     /locus_tag="ACHKAU_004429"
     CDS             4216..6309
                     /gene="flhA"
                     /locus_tag="ACHKAU_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5533365.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            6414..6692
                     /locus_tag="ACHKAU_004430"
     CDS             6414..6692
                     /locus_tag="ACHKAU_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO5533366.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            6685..7041
                     /locus_tag="ACHKAU_004431"
     CDS             6685..7041
                     /locus_tag="ACHKAU_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5533367.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(7098..7871)
                     /gene="yafL"
                     /locus_tag="ACHKAU_004432"
     CDS             complement(7098..7871)
                     /gene="yafL"
                     /locus_tag="ACHKAU_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5533368.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            8057..>8306
                     /gene="dinJ"
                     /locus_tag="ACHKAU_004433"
     CDS             8057..>8306
                     /gene="dinJ"
                     /locus_tag="ACHKAU_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO5533369.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDK"
BASE COUNT         1911 a         2148 c         2272 g         1975 t
ORIGIN      
        1 atttaatacc tcaccttgtg tcactgccgg atttgcgtgc acaaaatcgc ccataccatt
       61 acagcagaga aagtaagcca tgctgaagta cagtaaaacg ccaggcatct ttaaattaga
      121 aggtaatcgg ctcggacgcc cctaccatcg cctgccgacc ctgtttacag ggaattttga
      181 cgtgattgat tcgcatctgg gcagctactt tctcaaaaag catcgcagca atatcaccct
      241 aaaaaagatt ggctgcgaaa tggatattat taataaaaac gctgaactga tggtttccca
      301 ggtcgggcat ctggcgtttg atattgatcg ctctttgctg ttaatgctgc tgggcaattt
      361 ttatggtctg gagtcctctc ttgaagaggc gaaagcgcat aacgggctgc cgaccaaaac
      421 cgaaacgcgg ttaaaaaacc gtctggcgct ggatatctgc acgctgattt ttaatctgca
      481 aacctccggt attgcgctga aattaaagct ggacagcagc acggtaatta cccactgggc
      541 gtatcaatta acctttaccc ttgcgggcga tgaagagagc tgttttcgta ttttgctcga
      601 cgacgcccac accgatttta ttttaaatct gatccgccac agcgagcaca gtaaaccgca
      661 gcaggtggcg aaatcggtca ataaaccggc cctgattaaa gagatcatcc gctccctgcc
      721 gctgaccctg aacgtcaaaa tcgccgagct atcgatgaat gtcgcggatc tcacgcagat
      781 aaaagccggg gacattttac ccatctcgct gggagaaacg ttcccggtgg cgattgggca
      841 atctgaatta tttagcgccc tgattgtgga agacaaagac aaactgtttt tgtcggagct
      901 ggcgggcaaa aatgagaata gccatgagta agcaagaaga tattttagcc ggggattttg
      961 gcctcaccga tgaagttgcg cctgtcgcaa aatccgccga cgctgaaacg ctggtcaccc
     1021 gcctggaaga ccgtttttcc gactcgatga cgctgctaaa acgcattccg gtgaccttaa
     1081 cgctggaagt gtcatcagta gaaataatgt tagccgacct ccttaacatc gacgacgaca
     1141 cggtgattga gctggataaa ctcgccgggg agccgctgga tatcaaagtg aacaatatcc
     1201 tgctgggcaa agcggaagtg gtggtggtca acgagaagta cggcctgcgc gtgctggaat
     1261 ttaacacccg cgatatcaac gacctggcac catgaaacgc cgcactcaac tgacgctggg
     1321 ccttgggctg ctggcgcttg cgccggtggc gttggcccag ggcggcgata ttgcgctgct
     1381 taacgtggtc acccacggca acactcagga gtacagcgtc aaaatccagg tgctgattct
     1441 gatgaccctc gtcggtttgc tgcctacgat ggtgctgatg atgacctgct ttacgcgctt
     1501 tattatcgtg ctgtcgctgc tgcgccaggc gctagggcta caacaaacgc cgccaaaccg
     1561 cattctgatt ggcattgcgc tctccctcac catgctggtg atgcgcccca tctggctcaa
     1621 tatttacgac cacgcagtag tgccgtttga aaatgctcag attaccctga ccgatgcgtt
     1681 aagcaccgcc gcgacgccac tcaaacgctt tatgctggcg cagactgaca aaaaagcgat
     1741 ggcgcaaatc atgactatcg gcggcgcgaa gggccatgcc gccgaccagg atctctcgat
     1801 tgtggtgccc gcctatgtgt tgagcgagct gaaaaccgcc ttccagattg gctttatgat
     1861 ttacatcccg tttctggtta tcgacctgat tgtcgccagc gtactgatgg cgatggggat
     1921 gatgatgctg tcgccgctga tcgtttcgct accctttaag ctgatgctgt ttgtgctgat
     1981 cgacggctgg tcgctgacca tcggtacact caccaccagc attcgcgggc tggggctggg
     2041 ctgatgatgc tgaccataga cgtagccgcc gacattgtcg ccagcggcat aaaagtggtg
     2101 attttgctgg tttcggtgct ggtagtgccg agcctgctgg tcggcctgct ggtgagcgtg
     2161 ttccaggcgg taacgcagat taacgaacag accctaagct ttttgccgag gctgattgtc
     2221 acgctggtgg tgctgggcgt gtgcggtaaa tggatgatta tccagttgca tgatctctgc
     2281 attcacctgt ttacccaggc cgcgttgctg gttcattgag atgcgcacaa gcgacgttac
     2341 gcaactgatg gatctggcgc tggggctgtg gtttcccttt gtgcgcatta tggcgtttct
     2401 gcgctatgtg cctgtgctgg ataacagcgc cctgaccgtg cgcgtgcgga ttattttgtc
     2461 gctggcgctg gcaatcatca tcacgccgtt gatcccccat cctgttcccc acgacctgat
     2521 atcgctcaac agcctgatcc tgacggtgga gcaaatttta tggggcatgt tattcgggct
     2581 gatgtttcag tttttgtttc tggcgctaca gctggccggg caaattctct cgttcaatat
     2641 ggggatgagc atggcggtga tgaacgaccc gagcagtggc gcatcgacca ccgtgctggc
     2701 ggagctcatc aacgtctacg cgatcctgct gttttttgcc atggacgggc atctgctgct
     2761 ggtgagtgtg ctgtataagg gctttaccta ctggccgatt ggcaacgcgc tgcatccgca
     2821 aaccctgcgc accatcgcgc tggcctttag ctgggtgctg gcgtccgcat cgctgctggc
     2881 gctgccaacc acttttatca tgctgattgt gcagggctgc tttggcctgc tcaaccgcat
     2941 tgcgccgccg ctgaaccttt tttcactcgg ttttcccatc aacatgctgg cggggctggt
     3001 ctgctttgcc accctgctct acaacctgcc ggatcactat ctgcatctgg cgaattttgt
     3061 gttgcagcaa ctcgacgcgc tgaaaggtca ctatggcgga tagcagcagc gaagaaaaaa
     3121 cagagaaacc ctcggcgcaa aagctgcgta aagcgcgcga agaggggcaa ctgccgcgct
     3181 cgaaggatat ggggctggcg gcgagcctgt ttgcggcatt tgtggtgatc tccagcagct
     3241 tcccgtggta tgccgatttt gtgcgcgaga gttttatcag cgtgcaccag tatgcacagg
     3301 agattaataa cccagaggtg atcggccagt ttctgcgcca tcacctgtta attctcggca
     3361 agtttattct caccttgctg ccgatgcccg ccgccgcgct actctcctca ctagtgcccg
     3421 gcggctggct gtttctgcct aaaaaaatcc tcccggattt cagcaaaatc agcccgttaa
     3481 aaggcattga gcggctgttt tccagtgaac atctggcgga aacgggaaag atgacggtga
     3541 agtcggtggt ggtgctggtg atgctgtggg taagcctgcg caataacttt gcctcctttc
     3601 tcggcctgca ggcgctaccg ttcaaactgg cgatgaatga gggtttatcg ctctacgcct
     3661 gcgtgatgcg caactttgtc atcctgttta tcttctttgc gttaatcgac gtgccgctgg
     3721 cgaaagccct gtttaccaaa gggctgaaga tgaccaaaca ggagctgaaa gaagagtata
     3781 aaaaccagga aggcaagccg gaagtgaagg cgcgcgtgcg gcgtctgcaa cggcagttgg
     3841 cgatggggca aattcgcaaa gtggtgccga aagccaacgt ggtgatcacc aacccgaccc
     3901 actacgccgt ggcgttgcag tatgactcgt cgcgcgccgc cgcgccgttt gtggtggcaa
     3961 aaggcaccga tgaaatcgcc ctctatattc gccaggtcgc cgctgagaat caggtggaag
     4021 tggtcgagtt cccgcgactg gcgcgctcgg tttactacac cacccaggtg aaccagcaaa
     4081 ttccgtttca gctttaccgg gccatcgccc acgttctgac ctacgtcctg caaatgaaac
     4141 actggcgcga gggtgcacag ccgcgcccgg ccctgaacag acatatttcc attcccaaag
     4201 aggtgcttaa actggatggc gaaaacaact aaatcgttcc tcgcgctgct gcgcggcggc
     4261 aatcttggcg tgccgctggt gatcctgtgt attctggcga tggttatttt gccgctgccg
     4321 cctgcgctgc tggatattct gttcaccttc aacattgtgc tggcggtgat ggtgctgctg
     4381 gtggcggtgt cggcgaaacg accgctggaa ttcagcctgt tcccgaccat tttgctgatc
     4441 accaccttaa tgcgcctgac gctcaacgtc gcctctacgc gcgtggtgct gctgcacggg
     4501 catctcggcg cggacgcggc gggtaaggtg attgagtcgt ttggtcaggt ggtgatcggc
     4561 ggcaactttg ttgtcggctt tgtggtgttt atcatcctga tgatcatcaa ctttattgtc
     4621 gtcaccaaag gggccgagcg tatttccgag gtttcagccc gctttaccct ggatgcgatg
     4681 cccggtaagc agatggcgat tgacgccgat cttaatgccg ggttgatcaa tcaggcgcag
     4741 gcacaaaccc gacgtaaaga tgttgccagc gaggccgatt tctacggcgc gatggacggg
     4801 gcatcgaaat ttgtgcgcgg ggatgctatc gccgggatga tgattctggc gatcaacctg
     4861 atcggcggcg tctgtatcgg gatcttcaaa tacaacctga gcgccgacgc cgccttccag
     4921 cagtatgtgc tgatgaccat cggcgacggg ctggtggcgc aaatcccttc cctgctactc
     4981 tccaccgcgg cggcgattat cgtcacccgc gtcagcgaca acggcgatat cgcccatgac
     5041 gtgcgtcacc agttgctggc aagcccatcg gtgctctaca ccgctaccgg aattatgttc
     5101 gtactggcgg tagtgcctgg aatgccacat ctgccgtttt tgctgttcag cgccctgctt
     5161 ggctttaccg gctggcggat gagcaaacgc ccgcaggcgg cggaagcgga agagaaaagt
     5221 ctcgaaacgc tgacccgcac catcaccgaa accagcgagc aacaggtcag ttgggaaacc
     5281 attccgctga tcgagcccat cagcttaagc ctcggctaca agctggtggc gctggtggac
     5341 aaagcccagg gcaacccgct cacccagcgt attcgcggcg tgcgacaggt gatatccgac
     5401 ggcaacggcg tgctgctgcc ggagatccgc attcgggaaa acttccgcct taagcccagc
     5461 cagtacgcta ttttcatcaa cggcattaag gctgatgaag cggatattcc ggcggataaa
     5521 ctgatggcgc tgccctccag cgaaacctac ggcgagatag acggcgtgct ggggaacgac
     5581 ccggcgtacg ggatgccggt gacctggatc cagcctgcgc agaaagcgaa ggcgctgaat
     5641 atggggtatc aggtgatcga cagcgccagc gtgattgcta cccatgtaaa taagattgtg
     5701 cgcagctata ttcctgattt atttaactat gacgatatta cgcagttgca taaccgtttg
     5761 gcgtcgatgg caccgcgcct ggcggaggat ttaagcgcgg tgctcaatta cagccagttg
     5821 ctgaaagtgt accgggcgct gctgaccgaa ggcgtttccc tgcgcgatat cgtcaccatc
     5881 gccaccgtgc tggtcgccag tagcgcggtg accaaagatc atattctgct ggcagccgat
     5941 gtgcgcctgg cgctgcggcg cagcattacc catccgttcg ttcgcaagca ggagctgacg
     6001 gtgtataccc tgaataatga gctggaaaat ctgctgacta acctagtgaa tcaggcgcaa
     6061 cagggcggta aagtgatgct cgacagcgtg ccagtggacc cgaatatgct caaccagttc
     6121 cagagtacga tgccacaggt gaaagagcag atgaaagcgg cggggaaaga cccggtgctg
     6181 ctggtgccac cgcagctgcg ccctttgctg gcgcgttatg caaggttgtt tgcgccgggg
     6241 ctgcatgtgc tgtcgtataa cgaagtgccg gatgagctgg agttgaagat tatgggggcg
     6301 ttgagttgat gggggattct tttccatgct ttgcagcacc tgacaccatt aattgacgtc
     6361 acactgcaaa gagactacga tgacaccata caatgacgtc atggattgat agtatgatta
     6421 aggtcagcgc gctaggttct cgtcatagaa aaacattcga cgatgtaatg acggagccgc
     6481 caaaatctgg aattaaatgg gatgatgttg ttgcactcat taaagctgtg ggtggaacaa
     6541 taaaaaataa caacggctcc agacgaaaat ttcagattgg aacgaccaaa ttcagcacac
     6601 atgagccaca tccaaaaaat gtgatggata aaggcgcagt agcaggtctt aaggagtggt
     6661 ttgttaactg tgtaggggta gattatgaat aaagcatcga acattatcac aattaaaggt
     6721 caaccagccg cagtaacatt tgaagccgat atcaacgcat ttcgcggcaa gttcctcaat
     6781 gtaaacggtt attgtgactt tgtagccaca agtattgagg cattatatcg tgaaggcgaa
     6841 agcgcgctgg atgagtggat ggcggattgt gaagaggacg gtattgcccc ttttcgtgaa
     6901 gaagaagagc aaaaacgcct gacgcttcgt gttcctcatc acattgattc gcggctgacc
     6961 atcgtcgcca gacaacattc catttcaaaa aaccagctca ttgttgatgt cttagaacgt
     7021 gagttttcag cccagatgta aacaggtcgg atgccgcgtg gcatccgacc ttttgccagt
     7081 tcgtcaatat tacccattta caaaatcgtc tcctccgtca aaatccgccg cgcgcctaaa
     7141 aagtgatcct gccagaaagg ttcagctaac tgacttaccc gaatggtttc cccggtgcgc
     7201 ggtgactcaa taaactgacc gtcgcccaga tacaccccca tatggtcggc aatctcgcgg
     7261 ctgtggatat ggaaaaatag caaatctccc cggcgcaggt cgttattcgc cacaatggtt
     7321 gcccggcgat agtgatacat ctcattagcc gtgcgtggga gtttcgcctc aaggatcttg
     7381 ttgtaggcat aaaaaactaa cccgctacag tcgaagcctt gatcgggccg cgtaccgccc
     7441 cagacgtaag gcttgccaag ctgttgctcc agacggtgaa tcgccacttc ggtgatgtta
     7501 tgcagatggt cgctgcttaa gaaatggttg ttttccgcca gcgcctgcca gcgtctgtca
     7561 ctcgccttga gcggcgcagg aaaccattct ggatgctgtt ttatctgttc gcggttgtgc
     7621 tggcgtagcg cccttctgct ttcggcattg ccttccacaa tatagctggc ctgttttttc
     7681 agatgggttt ggtactgttt taataaacgc gcacgatttt gcatccgctg gcgggcggcg
     7741 tagctgaaag tgatatgtga tgtggtggcg ctggcaaatg aagaaaacgg caagcaaagc
     7801 aacagtaatc ccgacaacac aaaagaggga atcgacggca aggacatact caataatggc
     7861 tgatcatgca ttgatgttgc agtgtatccc ggcccgtacc tgaacaaact taataaacca
     7921 ttattataaa attaataaat atatttcagt taattatgta aaaacatccg tttattaaat
     7981 tactcatgga ttgtcgctgt tgctcatttg agctacaatt caagctgaat aaatatacag
     8041 cacaggagat accccaatgg ctgctaacgc atttgttcgc gcccgaatcg atgaagatct
     8101 gaagaatcag gcggcggacg tactggccgg gatggggctg accatctctg acctggttcg
     8161 cataaccctc acaaaggtcg cgcgtgaaaa ggcattgccg tttgatttac gcgagcctaa
     8221 tcaattaacc attcaatcaa tcaaaaacag cgaagctggc gttgatgttc ataaggccaa
     8281 agacgccgat gatttatttg ataaat
//
LOCUS       DAZFJF010000170         8287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFJF010000170 DAZFJF010000000
VERSION     DAZFJF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.171"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..241)
                     /locus_tag="ACHKAU_004434"
                     /pseudo
     CDS             complement(29..241)
                     /locus_tag="ACHKAU_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            391..1059
                     /locus_tag="ACHKAU_004435"
     CDS             391..1059
                     /locus_tag="ACHKAU_004435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5533370.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1004..1141)
                     /locus_tag="ACHKAU_004436"
     CDS             complement(1004..1141)
                     /locus_tag="ACHKAU_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO5533371.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1114..1698)
                     /locus_tag="ACHKAU_004437"
     CDS             complement(1114..1698)
                     /locus_tag="ACHKAU_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5533372.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1698..4658)
                     /locus_tag="ACHKAU_004438"
     CDS             complement(1698..4658)
                     /locus_tag="ACHKAU_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5533373.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAETPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSLSGSTSSAGAHSHVDGRRFNTSTFKDTYQYGSTAVGSNSYQVQGAVGLGI
                     GTMANTNSAGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAEN
                     TVKNIAFNYIVRLA"
     gene            complement(4723..5322)
                     /locus_tag="ACHKAU_004439"
     CDS             complement(4723..5322)
                     /locus_tag="ACHKAU_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5533374.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVSYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(5393..>8287)
                     /locus_tag="ACHKAU_004440"
     CDS             complement(5393..>8287)
                     /locus_tag="ACHKAU_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5533375.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDLEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPT
                     LRQRLFRCVSIRDNDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQH
                     LTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFT
                     QLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYD
                     PTVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGK
                     SAFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDA
                     NDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNET
                     PMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADMSGRVNANAG
                     TLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDD
                     HNFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPG
                     SYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT         1785 a         2333 c         2027 g         2142 t
ORIGIN      
        1 attcgaagta aatttataaa atatcaaatc aatatacatt ttgcaccttc gagatagtaa
       61 tgactgttac tgtaacatga gttacaaaca gcttcatacc cagagctact gatgtatcaa
      121 ctcgattcaa caatacccga tatttattcc atgcctccag caacgatctt tcttcctccg
      181 ttgcgatttc cagatctaca gcatcctgca gtggcgcaat atactcactg aattcctgga
      241 tgtagaactg tgtggtgacg gtcttccagc cattcggctc ctgttgtatc gaagcatacc
      301 aggctatttc aatatcgcta tgctgcggca gcatttaacc ccttgtaatt catcgccata
      361 attgatttaa ttcacaaata aaactataac atggtgaaat taatgaaaaa aaacacagat
      421 gatggggcta aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga
      481 gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg
      541 gaacatttag gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac
      601 gtacctgaga gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca
      661 tctacaaggg ctggggaatc aaaaattaaa cataaaatta gccatgatgt ttttgaacct
      721 tatcccgcgg cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc
      781 ctgcctggaa atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact
      841 gacgatggaa ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc
      901 ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc
      961 gaagaaggct taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa
     1021 ggtactgtag taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct
     1081 tgcttaagac gtgctgcggc ataatcccaa tgattactcc ctgacagggt tcgtaggcca
     1141 ctcaatatca ggtgcagttg atgtatcaac acggttcagc aacacccgat actttttcca
     1201 ggcttccagc aatgaggttt cttcctccgt tgcgatttcc agatctacag catcctgaag
     1261 tggcgcaata tgctcactgg ctacctgcat caggctgttt tttatttctt ccgcctctcg
     1321 gatccggaac agtttttctg cttctgcatc tttcacccag gctgtgccgt tccacttctg
     1381 aaactcccct tccggcgata accaggtaac attttccggt aacggaccga gttcagaaat
     1441 aaataacgcg tcccctgacg ctacgtcata aaccgtttta ccccgatggt cttcaacgag
     1501 atgccacgat gcctcatcac tgttgaaaac agccacaaag ccagcaggaa tatctggtgg
     1561 tgcaatatcg gtactgtttg ctggcagacc tgtatgaggc ggaatatatg catcaccttc
     1621 accaataaat tcattagttc cggccagcag attataaatt tttatggtcc gtgcttgttc
     1681 actcattctg aatgccatta tgcaagcctc acaatatagt taaatgcgat gtttttgacg
     1741 gtgttttccg cgttaccagc agcgttaacg gtgatggtgt gtccatgtga gccaatcgca
     1801 acggagtgcg tatgagcacc aataccgaca gtatgtgcgt gtgcacctgc gcttgcagca
     1861 gtgccggaca gtgagtgggt atgcgcgcca gcagagttcg tattcgccat cgtgccaatg
     1921 cccagaccaa ccgccccttg tacctggtat gagttacttc cgacagctgt agaaccatac
     1981 tgataggtgt ctttaaaagt gcttgtatta aacctgcgac catcaacgtg cgagtgtgct
     2041 ccggccgagc ttgtagagcc gctcagactg tgcgtatgag ccccggtgtt attcgtggat
     2101 ttagtgccgt aatcaaacga cgatgtggtt ttcgtcccca aatccgtact ggatgcgctg
     2161 gcgctgtggg tgtgcgattt aatgccgtcc tgttcctgag acaatacggc acgaccactg
     2221 gcgggtttgc ccttaatcgt ccagccacgc atatcaggga tcacgcctga cggataagcg
     2281 gctgcaagtt tcgggtatgc agatttgtca aaagtctgcc cctgcatcag ggcatagcca
     2341 gacggaacgg tatctgatgg ccacgggatt ggtgcgccag gcggataaaa ctgctctgat
     2401 ggcgtataga gtgaataaac tgtaccgtcc gttaaccctt ccggcttatt agcagaatat
     2461 gctggtgacg tatgaatcgt cacgctggca ttactggtat aatcccattg aatatttaca
     2521 ccagtcgcat aatttccgat tgcaacgtaa atatcgtaag tatcaccaga tgtattgacc
     2581 caggcaaaat ttgtaaaccc tgtcgatgtg cgctgccata aagcaccagt aatccccttc
     2641 ggattaccat tacctgcacg caaaacaagt tcagatatac ctgcctgttg aggtgacccc
     2701 acgttaaatc ccgcgccacc aatcaacgta attgaaacaa cagaactcgc ctgtggcatg
     2761 gttaccgttg ctaatttgaa ccaaccagca ccaccgctga atgacattgt tgttgagtta
     2821 agcgtaccaa tatctttcgg cgtcagtgtt atatccgctg aaagcgcctt accattcacc
     2881 ttacgggcag aaggtacccg accattcgca ttatcattag ctgctttcac tgctttcggt
     2941 gtctcggcaa gcgtttcaga tgcgctgttg gttgcactac tgagctggac aattcctttt
     3001 tgtgctgtcg tagcgtcctg agcggtatat ttcccgttag caaggtcata ggctgccttt
     3061 accgactttg gcgttgccgc cagcatttca gatgtgctgt tggtcgcgct gctgagctga
     3121 acgatccctt tctgtgctgt cgttgcatcc tgtgcggtgt atttcccgtt agccaggtca
     3181 tacgcggcct taacggcttt tggcgttgcc gccagtgact cggaagtgct gttagtcgca
     3241 ctgctgagct gtactatccc ctttttcgtc gtgctcgcat cctcaagcgc cacggcggat
     3301 gcaatatcct ctgcccgttt agctgctgtc tcggcgcgcg ttgccgcgga ttccgccgta
     3361 cttttgctct gagctgccgc cgtcgcactg ccagctgcct ctgtcgcctt cgtggatgcc
     3421 gtcgtggcgc tgctcttcgc tgctgacgcc tgtctggtcg cctcatcttt tgaagcagac
     3481 gccgatgatg ccgatgacgc cgccgaactg gctgacgatg cggcagccgt ttttgaggat
     3541 tctgcgctgg tttccgacgc tttcgcgttc gtttcggatg tcttcgctgc ggaagcagaa
     3601 ctcgctgctg cgctggcctg ttcagtggct tcgccagcct tcgttgtggc tgttgaagcg
     3661 gatgatgcgg cgctttctgc cgattttccg gcggcggtgg cactggctga ggcctgcccg
     3721 gcacttgttg acgcggcact ggcagacgac gcagccgctg tttttgagcc tgccgcagct
     3781 gaggcgctct gtcccgctgc cgtctcagaa gacttagcgt tcgtctcgga cgtttttgcc
     3841 gccttcgcgg aatttcctgc cgccgttgcc gaggaagcgg cactactggc gcttgatgat
     3901 gcgttcgttt ctgatgattt tgccgcttct tttgaagccg acgcatcccg ggctgaggtg
     3961 gcagcttctg acgctttcgt ggtcgcggtg gatgcagaag tggctgctga ttgttgtgac
     4021 gctgccgcat tcgtttctga cgttttcgcc gcagcggcac tggtggccgc cgcgcttttt
     4081 gaggactctg cagcggcagc actttttgat gcttcagtgg cctttgttga tgccgttcct
     4141 gcgctggaag acgctgactg agccgacgac gcggcctgtc cggctgacgt gctggctgcg
     4201 cgtgctgagc ctgcagcatc agtcgcatgg gttgccgcct cacgggctga tgtgctggca
     4261 tcgctggctg acttcttcgc ggctgccgtg ttctgtgcca ccgcggacgc gttacgcgcc
     4321 acctcttcca ccatcagttc aaaacggcgc agtgcctcag gacgggcatc atcctccgtc
     4381 atggcaccga gaaaatcatt cagcgtaccg ggtcgggaat cttcatacac ggtgatggtc
     4441 ccggcatgtg acggcgggaa gccttccacc aacagaataa cgctgtactg accgtactca
     4501 acgtccatgc tgtaacgccc ggcttcatcc ggattttctg aggccagcgt gttcaccacc
     4561 accgtggtac tgttacgttt tgctttcagc tggattgtgc agttctctac cggttttcct
     4621 gtgccgtctt tcagtacacc tgaaatcttt actgccatat tcaccccaca aaaaagcccg
     4681 cctgaaccgg cgggctgtca taacactgtg ttacctggct aatcagaatt tataaccgac
     4741 acccacgatg aaaccgtcag tgcgccagtc gccactgccg gagccttcat aagcaatatc
     4801 aatggccacg gattcggtcg ggttaaactg cacgccagct ccccacgcca gagacgtgtt
     4861 gctgtggcga ccgtcatcac ttccggtcag cacgtcgtgc gttttcccct tgttgtcagt
     4921 tacgcggaga taatccccgg agaaagtcga cacacggctg taagacacac ccgccatcgc
     4981 atacgcgctg aaccattcat tcacgcgcac agacggcccc gccatcacgc tgaaccagcg
     5041 gttacgcacg gaatcttcat gccagcggat atcgctgtaa cgggtaagct ggcgattctt
     5101 gtctcctgca tagctgaatg acgtcaccag ccccagtgtg tccgtaaact cataacggta
     5161 tttcacgtta atcccgttca gatcatcgct gccgggaacg ttcgtcgagg catgaagata
     5221 ccccgcgctc agcgtggact gatgttcaga tgcccatgca gacgcaccgg atacggccag
     5281 acaaatagct gcggacaaaa tggctgcata aagtttacgc ataattacct ctcgcttttc
     5341 tgcaataaaa aaggcgtcat tcctgacgcc ctttattggg gttataaata tttcaacgaa
     5401 tactgatacc ggaagccgct tttttggtca caatcaccgt acagtcggtg atattccctg
     5461 ccccctgatt gcctttctgg aaaatcttaa actccagagt gacgctacca ccaccactag
     5521 gcatatcaat aactgcactg taactaccgg gaatggcccc tttagtttct ctggatgcga
     5581 ttaatacacc gtttttgcga acttcaaaac cataacccgt gtatcttgta cctcccgggt
     5641 tattaccact tcccggatcg ctatacgcta ttccgttaaa gataatgggc ggaataatga
     5701 tttgacggtc aaagttatga tcatcgctga tggtgactgt aaccgtcccg tttggtgttt
     5761 ccgtattacc ccacgtacca gcctgtttcg ggaatgattt ggatacagct ttaacgaagt
     5821 cacctctgac ctgagtcgcc tccagcatgc ccttaatcgt acagttttca tttaccgtga
     5881 cattgttgag cgtcccggcg ttcgcattca ctctgccact catatccgca tttttagcgg
     5941 tcagctttcc gtctgatgtc agggaaaata ccggaggact gccaccgctg gtaatggtgg
     6001 gggctgtcag gcgcttcagg aacacgtcgt tcatgaatat ctggttgccc tgcgccacaa
     6061 acatcggcgt ttcattcccg tttgccgggt caataaacgc gatacggtta gcggcaacca
     6121 gaaactggct cagcttgcct tcctccgtgt cctccatgct gaggccaata cccgcgacat
     6181 aatgtttgcc gtctttggtc tgctcaattt tgacgcccca catggcattc catttatcgt
     6241 tagcgtcctt ccactctttc gaaaactcat ccagtctgct ggcgttatcc tccgtcaaat
     6301 caactttttc cagcagctct ttaccgagat gggattcggt tatcttgcct ttgaaaaaat
     6361 ccaggtaacc ttccgcatca tcgctcgccc gaccgacgcc ctccacgaat gccgatttgc
     6421 caacggtgtt cacactgcgg atataaaagt aataatcatg gcccggtttg atattgatac
     6481 tggcggctat ccagtacagc gccgtaccaa gatagcgggc tgtggtttca acctgcctga
     6541 tatcggtaat ccgcgtttcc gagaaccaga actcaaactg taccgtcggg tcataaacgg
     6601 caagatgcgg cgtggcggtt atctgaaaat agcccggcgt cagctcaatc ctcgacggtg
     6661 ctgccggtgc ggcaatccgg aacgataccg acgccggatc gccctgctgc ccccacgcat
     6721 ttaccgcccg gactgtcagc ctgtagttcc ccagcgccag ttgcgtgaag cggtatgtgg
     6781 tttccgtcgt ccgggccgtg ctgaccagcc gctcactgcc gtcatccgct gttacggtca
     6841 gacggagcag gaagctcacg cccttcacca ccttcggcgt gtcccagcgc gccagcacct
     6901 gatattcccc gctgtctgcg gtgacttcgg cggtcaggtg ctgcaccgct ggcggcgtga
     6961 caccattcac cgtgccgctc tggtcgccgt caaagtgcgc cccgttatcc acgatggctt
     7021 ctttttccgg tacatgctgc acggcagtga tggcatacgt gccgtcatcg ttatcacgga
     7081 tactcacaca gcggaacagg cgctgacgca acgtcggcag cttcagcccc cacacactgt
     7141 attctgcaac gccgtcagga acccggttca ctttcacctt aagtccgtcg gtgacggact
     7201 gaacctccac gctgaccgga ttaccacttc cgtcaaccag gcttatcagc gtggtaccgg
     7261 aggatggcag cgtgatttca cggtcgagcg tcagcgtccg ggtctggctg ttcaccgcca
     7321 gcacgcgacc accggtgctg ataccggcat agtcatcatc gaagatttca ataacatcgc
     7381 ccggtacatg gcgaagccct tcagcaccca cgctgaaatc cacggtctgc gtctccagca
     7441 gttccgtttt aatcagccac agcccggcgc ggtgtgcctg cccccggctg gtacagccaa
     7501 aggcatccat cttcgtgaca ttacgaccgt aacgggcaat ggcctgcgta tcttcaacaa
     7561 gctctgtcgc cgtctcccag ccgttgttcg ggtcaatcca gttcacctca acggcattat
     7621 ggcggtcctt cagggcgctg aagctgtagc ggaacggcgc gccatcatcc ggcatcacca
     7681 cattactgcg gttataggtc cacaccttat ctgatggtcg gtcctgcacg aacgtcagcg
     7741 tctgcccgtt ccataccggc atacagcgca tcgccgagca gaaatcactg agcacatccc
     7801 acgccttgcg ctgtgtggtc aggtatgcat tacaggtgat gcgcggctcc gtgccgccaa
     7861 agccgtccgg tactgactgg tcgcagtact ggccgatgac atacagcgcc catttgtcca
     7921 catccgccgc accaagacgt ttccccatgc cgtagcgtgg atgggtcagc atatcccaca
     7981 gacaccaggc catgttgttg ctgtatgccg gtttaaacgt tccgtcccag ataccgctgt
     8041 attgtcgcgt ctgcgggtta tagttcgacg gcacctgcag aatgcgcccg cgaagatgat
     8101 aattacgact cacctgctgg ctgccgaact gctccaagtc cacctgtacg ccgaccagtg
     8161 ccgtgttcgg gtagcactgt ttcacatcga tgatttcggt gtatgacgac cagagcgttt
     8221 tgttctgcag ctggtctgtg gtgctgtccg gcgtcatcct gcgcatccgg atattaaacg
     8281 ggcgcgg
//
LOCUS       DAZFJF010000171         8286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFJF010000171 DAZFJF010000000
VERSION     DAZFJF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.172"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..76
                     /gene="yobI"
                     /locus_tag="ACHKAU_004441"
     CDS             11..76
                     /gene="yobI"
                     /locus_tag="ACHKAU_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO5533376.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            215..826
                     /gene="ruvA"
                     /locus_tag="ACHKAU_004442"
     CDS             215..826
                     /gene="ruvA"
                     /locus_tag="ACHKAU_004442"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO5533377.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            835..1845
                     /gene="ruvB"
                     /locus_tag="ACHKAU_004443"
     CDS             835..1845
                     /gene="ruvB"
                     /locus_tag="ACHKAU_004443"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO5533378.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1992..2777)
                     /gene="znuB"
                     /locus_tag="ACHKAU_004444"
     CDS             complement(1992..2777)
                     /gene="znuB"
                     /locus_tag="ACHKAU_004444"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO5533379.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2774..3529)
                     /gene="znuC"
                     /locus_tag="ACHKAU_004445"
     CDS             complement(2774..3529)
                     /gene="znuC"
                     /locus_tag="ACHKAU_004445"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO5533380.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3608..4540
                     /gene="znuA"
                     /locus_tag="ACHKAU_004446"
     CDS             3608..4540
                     /gene="znuA"
                     /locus_tag="ACHKAU_004446"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO5533381.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4556..5878
                     /gene="mepM"
                     /locus_tag="ACHKAU_004447"
     CDS             4556..5878
                     /gene="mepM"
                     /locus_tag="ACHKAU_004447"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO5533382.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5998..6969
                     /gene="lpxM"
                     /locus_tag="ACHKAU_004448"
                     /gene_synonym="msbB"
     CDS             5998..6969
                     /gene="lpxM"
                     /locus_tag="ACHKAU_004448"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5533383.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7058..8286)
                     /locus_tag="ACHKAU_004449"
                     /pseudo
     CDS             complement(7058..8286)
                     /locus_tag="ACHKAU_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2214 a         1951 c         2071 g         2050 t
ORIGIN      
        1 aggagtcgca atgtatattt ttattacgca tttcttcact gaatatgtaa tattcaaata
       61 tttgcttcca atataacctg tagaataaat tatactgcgc catttttcag atcgttggga
      121 cgcctcgcaa gttttcttca tccttcgctg gatatctatc cagcattttt ttatcataca
      181 gcattatctt tgattcatta cgcaggagcg tcatgtgata ggcagactca gaggcatcat
      241 cattgaaaaa caacccccgc tggtgttaat tgaagtgggc ggcgtaggct atgaagtgca
      301 tatgccgatg acctgttttt atgaactccc tgaagcgggt caggaagcga tcgttttcac
      361 ccactttgtg gtgcgtgaag acgcgcaact gctgtacggt tttaacaata aacaagagcg
      421 cacattgttc aaagagttga tcaaaaccaa cggcgtcggc ccgaagttgg cgctggcgat
      481 cctctccgga atgtcagcgc agcagttcgt taatgccgtt gagcgtgaag aagtgggggc
      541 actggtgaaa ctgccgggta ttggcaaaaa aaccgccgaa cgcttgattg ttgaaatgaa
      601 agaccgattt aaaggtttgc atggcgatct ctttacgcca gccgccgacc tggtactcac
      661 gtcacctgcc agcccgacga ccgacgatgc tgaacaggaa gcggttgccg cgctggtggc
      721 gctgggctat aaaccacaag aagcaagccg catggtgagc aaaatcgctc gccctgacgc
      781 cagcagtgaa acattaattc gcgaagccct acgcgccgcg ttatgaggta aaggatgatt
      841 gaagcagacc gtctgatttc tgccggtacc actttgccgg aagatgtggc agatcgcgcc
      901 attcgcccta aattactgga agagtatgtt ggtcagccgc aggttcgttc acagatggag
      961 attttcatca aagcagcgaa actgcgcggc gatgccctcg atcatctgtt gattttcggt
     1021 cctccggggt tgggtaaaac tacgcttgcc aacattgtcg ccaatgaaat gggcgttaat
     1081 ttacgcacga cttctggtcc ggtgctggaa aaggcgggcg atctggcagc gatgctcact
     1141 aaccttgaac cgcatgacgt gttgtttatt gatgagatcc accgtctttc gccagtggtg
     1201 gaagaggtgt tgtatccggc aatggaagac taccaactgg atatcatgat tggtgaaggt
     1261 ccggcggcac gctccattaa aattgatttg ccgccgttta ccctgattgg tgcaaccacg
     1321 cgcgcaggtt cgctgacatc accgttgcgc gatcgttttg gtattgtgca gcgtctggag
     1381 ttttatcagg tgccggatct gcaatatatc gtcagtcgca gcgcacgctt tatggggctt
     1441 gagatgagtg atgacggcgc gctggaagtt gctcgtcgag ctcgcggtac gccgcgcatt
     1501 gccaaccgtc tgctgcgtcg agtgcgtgat ttcgccgaag tgaagcacga tggcaccatc
     1561 tcggcagata tcgctgctca ggcgttggat atgttgaatg tcgatgctga aggtttcgat
     1621 tatatggacc gcaagttatt gctggcggta atcgataagt tctttggtgg gccggtaggt
     1681 ctggataacc ttgcggcagc cattggcgaa gaacgtgaaa ccattgagga tgtgctggaa
     1741 ccttatttga ttcagcaagg ctttttgcag cgtacaccgc gtgggcgtat ggcgacgacg
     1801 cgggcgtgga atcactttgg cataacgccg ccagaaatgc cgtaagtcgg attggtcgct
     1861 cgcgccgcat ccgacactgg cagtgtgtct gatgcgacac tgttcgcgtc ttatcaggcc
     1921 agccagagat agcgaaccgt aggtcggata aggcgctcgc gccgcatccg acaaatgtgt
     1981 tcagcgatag attagctggc ctgctttttc atcatactga gaataaataa cagtgccgca
     2041 catagcacca ccgacggacc cgccggcgta tcgtaaaacg cggaaaaggt taaaccgcca
     2101 gttactgcca ccatccccac caaaacagcg acaccagcca tctgttccgg cgtacgggca
     2161 aagcgacgcg cagtagctgc aggaataatc agcagtgaag taataatcaa cgcgccaaca
     2221 aatttcatcg ctacaccaat cgtcaatgcc gtcaccagca tcaacaacaa tttcacgcgc
     2281 tgtaatttca caccatcaac aaacgccaga tccgggctaa tcgtcatcga cagcaaattg
     2341 cgccattgcc agaacaaaat agccaccacg atgaccacgc caatcgcaat agcgatgaga
     2401 tcttctggcg tcactgccag caaatcaccg aacaggtaag ccatcaaatc aacacgaatg
     2461 ttagacatca gactaacgac caccaggccc agcgacaggg cactgtgcgc cataatccct
     2521 aataacgtgt caatcgccag ctgtggacgc ttctccagcc ataccagacc gcccgccagg
     2581 agcagcgtaa cggcaattac cgcatagaat ggattcacgt ccagcaacaa accaaacgcg
     2641 acgccaagta atgaggcatg agccagcgta tcaccgaaat aagacatacg acgccagact
     2701 acaaacgaac ccagcggacc cgcggcacag gcgagcatga tcccggctaa ccaaccggga
     2761 aataataatt caatcatgag cgatcatttc cccgacgcaa aacaattcgt ccctgtaaat
     2821 cgtgacgatg attatgatga tggcgataga tacccagttg ttcagcacca cgaggaccaa
     2881 acatagaaat aaactccgga tgtagggaaa caacttccgg tgtgccggaa caacaaatgt
     2941 ggtgattcag acacagcact tcatcggttt ttgccattac cagatgcaga tcgtgagaga
     3001 ccattaaaac gccacaatcc agttcgcgac gcaactggtc aataaggtca tataacgcca
     3061 cctggccatt tacatccacg ccttgagtgg gttcatccag cactaataat tgcggtcgat
     3121 ttaacaatgc tcgcgctaac agtacacgct gtgtttcgcc accagagagc ttttgcatcg
     3181 gtgcgttaat cagatgcccg gcctggacac gtttcagtgc aggcaaaata tcttctttat
     3241 gtgtgccagg gcgtaagcgt aaaaaacggt ttacggtcag tggcaacgtg gtgtcgagat
     3301 acagcttctg cggtacatag ccgatgcgta gttttccgtt gcgcttgata accccttcat
     3361 cgggtgttac cagcccgagc actacccgta ccagtgtcga cttacctgcg ccatttggcc
     3421 caagtaaagt caaaattttt ccaggtttaa gttccagcga cacatcagag aggacgcggc
     3481 gttggccaaa agaaaccgag acattttcca gggaaaccag acttgtcatg ttaattttag
     3541 tcttgcagta gtcatgaaat gttataatat cacacttctc atattcatta cgatgattgg
     3601 tcgcattatg ttacataaaa aaacgcttct tttcgcagca ttatccgccg ctctctgggg
     3661 aggtgcaaca caggccgcag atgccgccgt tgtcgcttcg cttaaacccg ttgggttcat
     3721 cgcttctgcc attgctgatg gtgtaacaga aacggaggtt ttactgcctg acggcgcttc
     3781 agaacatgat tattcactgc gtccatcgga tgtaaaacgc ttacagaacg cggacttagt
     3841 cgtttgggtt ggcccggaga tggaagcgtt tatgcaaaaa ccggtaagca aattaccagg
     3901 agcgaagcag gtaacgattg cgcagcttga agatgtgaaa ccgctactga tgaaaagtat
     3961 tcacggcgat gatgatgatc acgaccacgc ggaaaaaagt gacgaagatc accatcacgg
     4021 cgatttcaac atgcatcttt ggctttcccc agagatagcg cgggctacag cggttgcaat
     4081 ccatggaaaa ttagtggaac ttatgccgca aagtcgagcc aaacttgacg ccaacctgaa
     4141 ggattttgag gcacaattag cctcaaccga aacgcaggtt ggtaacgagc tcgcgccgct
     4201 caaggggaaa ggttatttcg tttttcacga tgcttacggc tatttcgaaa aacagtttgg
     4261 actgacaccg cttggtcatt ttaccgttaa ccctgagatt caacctggcg cgcagcgttt
     4321 acatgaaata agaacacagt tggttgagca aaaagcaacc tgcgtttttg ctgagccaca
     4381 gttcaggcca gcggtcgttg agagcgtcgc acgagggaca tccgttcgta tgggaacgtt
     4441 ggatcccctg gggacgaata tcaaactggg taaaacaagc tattcagaat tcctgagtca
     4501 attagccaac cagtatgcga gctgcctgaa aggagattaa tgaggaagtg attacgtgca
     4561 acagatagcc cgctctgtcg ccctggcgtt taataattta ccgcgaccac accgcgttat
     4621 gttggggtcg ctcaccgttc ttactctggc cgtcgctgtc tggcggcctt atgtttatca
     4681 ccgtgatgcc acgccaattg tcaaaaccat tgagctggaa cagaacgaaa ttcgttcgct
     4741 cttacctgaa gccagtgagc cgattgatca agctacacaa gaagatgaag ccattcccca
     4801 ggacgaactg gatgacaaaa tcgccggtga agcgggcgtg catgaatatg ttgtttccac
     4861 tggcgatacg ctaagcagca ttctcaatca gtatggtatt gatatgggtg atatcaccca
     4921 actggctgcg gccgacaaag aattgcgtaa cctgaaaatc ggtcaacaac tctcctggac
     4981 attaaccgcg gacggcgaac tgcagcgcct cacctgggaa gtgtctcgtc gtgaaacccg
     5041 aacctatgac cgtactgccg ctaacggttt taaaatgacc agcgaaatgc agcaaggaga
     5101 gtgggttaac aatctgctga aaggtaccgt cggggggagc tttgttgcca gcgccagaaa
     5161 cgccggttta accagcgccg aagtgagcgc agtgattaaa gccatgcagt ggcaaatgga
     5221 tttccgcaaa ctgaaaaaag gcgatgaatt tgcggtgtta atgtctcgag aaatgcttga
     5281 tggtaaacgt gagcaaagcc agctgctggg cgtacgtttg cgttcagaag gtaaagatta
     5341 ttacgctatc cgcgctgaag atggcaaatt ctacgatcgt aacggtactg gtctggcgaa
     5401 aggattcttg cgattcccga cggcgaaaca gttccgtatc tcgtctaact ttaacccgcg
     5461 tcgaactaat ccggtgacag gtcgcgttgc accacacaga ggtgttgatt tcgccatgcc
     5521 acaaggtacg ccagtgcttt cagtgggtga cggtgaagtg gtggttgcca aacgcagtgg
     5581 cgcagcaggt tattatgtgg ctattcgtca tggtcgcagc tacaccacgc gttatatgca
     5641 cttgcgcaag attctggtga aaccgggaca gaaggtgaaa cgtggcgacc gtatcgcgct
     5701 ttccggtaat accggacgtt caactgggcc gcatctgcac tatgaagtat ggataaacca
     5761 gcaggccgta aacccgctga cggcaaaact gccgcgtact gaagggctga ccggctccga
     5821 tcgtcgcgaa ttcctggcgc aggccaaaga gattgtgccg cagctacggt ttgattaatt
     5881 aacatccatt cgcagccggt acgcagtcag taccggcttt ttttatttgg tgcggggcaa
     5941 gttgcgccgc tacactatca ccagattgat ttttgcctta tccgaaactg gaaaagcatg
     6001 gaaacgaaaa aaaataatag cgaatacatt cctgagtttg ataaatcctt tcgccacccg
     6061 cgctactggg gagcatggct gggtgtagca gcgatggcgg gtatcgcttt aacgccgcca
     6121 aagttccgtg atcccattct ggcacggctg ggacgttttg ccggacgact gggaaaaagc
     6181 tcacgccgtc gtgcgttaat caatctgtcg ctctgctttc cagaacgtag tgaagctgaa
     6241 cgcgaagcga ttgttgatga gatgtttgcc accgcgccgc aagcgatggc aatgatggct
     6301 gagttggcaa tacgcggacc ggagaaaatt cagccgcgcg ttgactggca agggctggag
     6361 atcatcgaag agatgcggcg taataacgag aaagttatct ttctggtgcc gcacggttgg
     6421 gccgtcgata ttcctgccat gctgatggcc tcgcaagggc agaaaatggc agcgatgttc
     6481 cataatcagg gcaacccggt ttttgattat gtctggaaca cggtgcgtcg tcgctttggc
     6541 ggtcgtctgc atgcgagaaa tgacggtatt aaaccattca tccagtcggt acgtcagggg
     6601 tactggggat attatttacc cgatcaggat catggcccag agcacagcga atttgtggat
     6661 ttctttgcca cctataaagc gacgttgccc gcgattggtc gtttgatgaa agtgtgccgt
     6721 gcgcgcgttg taccgctgtt tccgatttat gatggcaaga cgcatcgtct gacgattcag
     6781 gtgcgcccac cgatggatga tctgttagag gcggatgatc atacgattgc gcggcggatg
     6841 aatgaagaag tcgagatttt tgttggtccg cgaccagaac aatacacctg gatactaaaa
     6901 ttgctgaaaa ctcgcaaacc gggcgaaatc cagccgtata agcgcaaaga tctttatccc
     6961 atcaaataaa aaacagcctc tcaaattatt gagaggctgt acttgataaa aaatctcaat
     7021 gagtgttctc accattttga gtttttagat tagtacgcta ttgagttaat cgtagcgtga
     7081 taatttcaaa aggtttgaat tcaagattgc aatattgatt gcaataaaaa ctgtcatcta
     7141 atttattttc ttggagatcg acatattgaa cgtagtgata attacatacc cacgaaaaca
     7201 tggctgaaat ttctaatcca taactctcat ataatcttac gattataccg tcgccgtctt
     7261 cagctaattt tattgtttcg atgataacgc tgttagtatc gatagcaaaa aatgatagtg
     7321 aagggggtag ttcacccggg tgtggttgac agggacgatc tgtaagggat gattcattat
     7381 ataggcctgg ttaacgacac cacccacacg gtaatctcca tgatgaggaa ataaggagaa
     7441 tgtgaatttt tgtttaccaa tatctgcaaa attattaatc aggaaattat cataattatc
     7501 tgtcttaaca acttccccag gtttgtgctg cgaacgcaac aaagttaact ctagcgtctg
     7561 gtctttgaca cgatagccat atttgttttt tgctattaaa gcaatgccag tttcttgatc
     7621 agaaaggtcg acccagtgat gtgctggtat ttcatctttg gcaaaagcga acgaatcatc
     7681 gctatgcgtt ggtctgtcaa ttacaccgaa ctgaatttga cattgtgcaa gagatgtttg
     7741 tactgatacc gggaatttta ctttgagaga aacattaggt tcgtgccaat caataaaagt
     7801 ttggacatca acgcgtggac tgtcctgtgt cagggtaaat gcgaagcgaa gataagagga
     7861 attgtagtga taaatcagcg tcatcgtagt tttaggcccg ctgatcacag ttgtcatttt
     7921 ttcagcgagt aaaacatatg attccccatc acgataattt gaagcaaaat cccaggccgc
     7981 gaaaaaacct gcatcttcgt ggtatgcacg gatctcactg tgcatatttt cagtaatgta
     8041 ttctttacca tatcttttat catatagcga aattaaatca ccgctttctg aaaaaagaag
     8101 tttaatgtaa ttattttcaa tacttcgaga ttctgccttc agaccattta cgatcttatt
     8161 ctttggatca atcacagcat aaccataaga gtttactcgt gctttgagcc agttgttgtt
     8221 tatattgatc cattcgttac gagcaaaaca ggtggtatta aataaaatgt gcggatcttt
     8281 ataaga
//
LOCUS       DAZFJF010000172         8242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFJF010000172 DAZFJF010000000
VERSION     DAZFJF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.173"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..269
                     /locus_tag="ACHKAU_004450"
     CDS             <1..269
                     /locus_tag="ACHKAU_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533384.1"
                     /translation="RKDNIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYK
                     SRHKMLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            492..989
                     /gene="ubiC"
                     /locus_tag="ACHKAU_004451"
     CDS             492..989
                     /gene="ubiC"
                     /locus_tag="ACHKAU_004451"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO5533385.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1002..1874
                     /gene="ubiA"
                     /locus_tag="ACHKAU_004452"
     CDS             1002..1874
                     /gene="ubiA"
                     /locus_tag="ACHKAU_004452"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO5533386.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2029..4452)
                     /gene="plsB"
                     /locus_tag="ACHKAU_004453"
     CDS             complement(2029..4452)
                     /gene="plsB"
                     /locus_tag="ACHKAU_004453"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO5533387.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4623..4991
                     /gene="dgkA"
                     /locus_tag="ACHKAU_004454"
     CDS             4623..4991
                     /gene="dgkA"
                     /locus_tag="ACHKAU_004454"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5533388.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5101..5709
                     /gene="lexA"
                     /locus_tag="ACHKAU_004455"
     CDS             5101..5709
                     /gene="lexA"
                     /locus_tag="ACHKAU_004455"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO5533389.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5782..7107
                     /gene="dinF"
                     /locus_tag="ACHKAU_004456"
     CDS             5782..7107
                     /gene="dinF"
                     /locus_tag="ACHKAU_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO5533390.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7223..7432
                     /locus_tag="ACHKAU_004457"
     CDS             7223..7432
                     /locus_tag="ACHKAU_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO5533391.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7474..7989)
                     /gene="zur"
                     /locus_tag="ACHKAU_004458"
     CDS             complement(7474..7989)
                     /gene="zur"
                     /locus_tag="ACHKAU_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO5533392.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1863 a         2006 c         2252 g         2121 t
ORIGIN      
        1 cacgaaagga taatatcttt atcctgttct cctctgccga tgatgggcca gttatggtgg
       61 taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca
      121 cgtgtatcta taaatccaga cataagatgt tgcctattaa tcttactcag gaaacacttt
      181 ttagctccaa atctcatggt aaatatgcgc ttttcccaat ttttactgcg agttggcgag
      241 ctactcgtat aaagaatata ggtatttaag taaaggaaaa catcaccgtt cctggcatca
      301 tgggcggtga tgcccctacg gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt
      361 caacagcggt ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa
      421 tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg
      481 gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag
      541 agatccctgc cctggatccg caactgctcg actggctgtt gctggaggat tccatgacaa
      601 aacgttttga acagcaggga aaaacggtaa gcgtgacgat gatccgcgaa gggtttgtcg
      661 agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac
      721 gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg
      781 tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac
      841 gctatctgtt cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg
      901 gactgtgggg gcgacgttcc cgcctgcgat taagcggtaa accgctgttg ctaacagaac
      961 tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc
     1021 agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac
     1081 tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt cctcagctct
     1141 ggatcctggc ggtatttgtc gcgggtgtct ggctgatgcg cgctgctgga tgtgtggtga
     1201 atgattatgc tgaccgaaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc
     1261 ccagcggcgc ggtaacagag aaagaggcgc gcgcactgtt tgtagtgctg gtactgattt
     1321 cgtttttact ggtactgacg ctgaatacga tgaccattct gttgtcgatt gccgctctgg
     1381 cgttggcgtg ggtgtacccg tttatgaagc ggtataccca tctcccgcaa gtggtgctgg
     1441 gcgcggcgtt tggctggtcg attccaatgg cttttgccgc tgtgagtgag tcggtgccat
     1501 tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt
     1561 atgcgatggt tgaccgcgat gatgatgtga agattggcat taaatccacg gcaatcctgt
     1621 tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg gcactgatgg
     1681 cgatcatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt gtggtggctg
     1741 gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta
     1801 aagcatttat gaataataac tatgttggtc tggtactatt tttagggctg gcaatgagtt
     1861 actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc
     1921 tgatgcgcgg cgcttctcag gcctacacaa cacatcgcaa tttattgaat ttgcagatta
     1981 tggaaggccg gataaggcgt tttcgccgca tccggcaatt ctctctgatt acccttcgcc
     2041 ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg
     2101 ataaaccttc atcgtttctg ccggttcggc atcgccgcta tcgctgatat acccttcatc
     2161 acgcagtgtc agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat
     2221 gccgtgcagc acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg
     2281 gttgatcgac gggttggcac tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc
     2341 gcgcgcgcct gcggcgagca gctgtagcgt gcgagaatgc accgggttga tatgcaactc
     2401 atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac
     2461 gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag
     2521 cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc
     2581 cgccatcagc gaaggcagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag
     2641 caccgcttgc tcgcgtggca gaatgatgat gtcgccgata gtgtctttct cgacttcaaa
     2701 cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga
     2761 gtccgtggag taaggtacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa
     2821 ctgctcgcgg gtgagtgagc gctgacgtga tgccagtagc gcagtacagc acaggttcat
     2881 ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac
     2941 cgtcggcgtt aaccaggccg gacgcaccgc ttcgatggga tcgatagatt cacgccagtc
     3001 tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc
     3061 ctgaccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc
     3121 tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc
     3181 gatatagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt
     3241 catcgacagc gtaccggttt tcggatccag caaacgcccc gtgcgggaac gaccgccttc
     3301 cacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac
     3361 ggtggaataa agtttattgc ctttaaaagt acggcgaata aagaacgccc ccagacggcg
     3421 gaaaatcggc ccggccggcc agaagttcag gttgatcccg gcggcgatat gcggcggcac
     3481 cagcccctgg tggtacagca cgtaagaaag tagcaggtag tccatgtgac tgcggtggca
     3541 aggcacatac accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg
     3601 gacgttgatg ccctgataaa gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg
     3661 gatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg
     3721 cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc
     3781 gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg ggcccacagc
     3841 agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gagcgatagt
     3901 tttatctgtg ccgtgttcat cggccatgcg gcgcagagaa actgacggcg agaaacgcac
     3961 aaaactgtcg cgaccgagcc acagtacggc gaaaaatttt tgtacgccgt taagcatacg
     4021 cagcggcggg tttacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac
     4081 tggcaccatc tgcacatcca gatttgggtt gctgcggtgc aaatcaagat agtcgtggaa
     4141 cagcttaata gactcttctt tcggcgtgta ataggtgaac acacgcggcc cgccgtgaat
     4201 gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa
     4261 gtcatgagcc agacactggg cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa
     4321 aacgtacata attggacgag aggtatccag ccccagttcc ggggcaggat ctgccggaat
     4381 agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg
     4441 ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata
     4501 gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac
     4561 ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt
     4621 caatggccaa taataccact ggattcaccc gaattatcaa agctgctggc tattcctgga
     4681 aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat
     4741 tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc aattacccgc gtgctgctta
     4801 tcagctccgt gatgctggtg atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg
     4861 ttgaccgaat tggctctgaa taccatgagc tttccggacg cgcaaaagat atgggatccg
     4921 ccgcggtgct gattgccatt atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc
     4981 attttggata acccttccag atttcgataa atctctggtt tattgtgcag tttatggttc
     5041 caaaatcgcc ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga
     5101 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     5161 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     5221 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc
     5281 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt
     5341 cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc
     5401 gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
     5461 aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt
     5521 aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa
     5581 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac
     5641 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac
     5701 tggctgtaac atatctctga gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct
     5761 ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc actctggcat
     5821 ctcgccttac ccatgatttt ctccaatatc accgttccgt tgctgggact ggtcgatacg
     5881 gcggtaattg gtcatcttga tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg
     5941 gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact
     6001 gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaaccgttg
     6061 ctgttggcgt tgggggctgg ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg
     6121 gcgctgcata ttgttggcgg tagtgaagcg gtcctggaac aggcgcggcg ctttcttgaa
     6181 atccgctggt taagcgctcc ggcgtcgctg gcgaatctgg tattactcgg ttggttactc
     6241 ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt
     6301 gtgctggatg tctggctggt gatggggctg catatgaacg tgcagggcgc ggcgctggcg
     6361 acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc
     6421 aaactacgcg gaatttccgg cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc
     6481 ttgctggcgc ttaaccgcga tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc
     6541 gcgatcaccg tacttggtgc gcgactgggg agtgacattg tcgctgttaa cgcggttctg
     6601 atgacgctac tcacctttac cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg
     6661 cactccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt atggcgggca
     6721 gcgtgccgcc agtcggggat tgtagcgtta ctgttttcgg tggtttattt gctggcaggg
     6781 gaacacatca ttgcgttgct gacgtcgtta acccagattc agcagctggc tgaccgctat
     6841 cttatctggc aggtgatttt gccgttggtt ggcgtctggt gttatctgct ggacggcatg
     6901 tttataggcg caacgcgtgc cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt
     6961 tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gctggcatta
     7021 accgtctttc tggcgttgcg cgggctttct ctggcggcta tctggcggcg tcactggcgc
     7081 aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaata atcctaagcc
     7141 aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca
     7201 acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt
     7261 taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga
     7321 aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca
     7381 ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc
     7441 tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac
     7501 agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc
     7561 acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag
     7621 cgtatgcata atgtcttcca cgccttctgc acactcttct ttcaccgcgc cgcagcgatc
     7681 gcaaataaac atggctgacg tatgggtggg ctggtcgaac agatgacaga gcacataact
     7741 gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata
     7801 aaccgttggc ggcttggctt gcggttcagc ttcgcgcagt aaatcaagca gatcataagc
     7861 gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag
     7921 gcgcacatta cgctgcgcgc agattttttc agcctgcgct aataactcct gcgttgtggt
     7981 cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag
     8041 gaaaaacacc gagaaccctt attgttgccg taatgttgat tttctgtttt gtaggtaagg
     8101 tgttgtgttg ccttgtcgta ccattatcaa cacgataata attaataatt catttttaaa
     8161 taaattgctt caagttaact cgctatttat ttaactgggt ttaaacgcat acctgtagtt
     8221 cagaaattat gagcgatttt tg
//
LOCUS       DAZFJF010000173         8177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFJF010000173 DAZFJF010000000
VERSION     DAZFJF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.174"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..944
                     /locus_tag="ACHKAU_004459"
     CDS             114..944
                     /locus_tag="ACHKAU_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5533393.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            976..1911
                     /locus_tag="ACHKAU_004460"
     CDS             976..1911
                     /locus_tag="ACHKAU_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5533394.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            2004..2246
                     /gene="yhdT"
                     /locus_tag="ACHKAU_004461"
     CDS             2004..2246
                     /gene="yhdT"
                     /locus_tag="ACHKAU_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO5533395.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2236..3687
                     /gene="panF"
                     /locus_tag="ACHKAU_004462"
     CDS             2236..3687
                     /gene="panF"
                     /locus_tag="ACHKAU_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO5533396.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3699..4580
                     /gene="prmA"
                     /locus_tag="ACHKAU_004463"
     CDS             3699..4580
                     /gene="prmA"
                     /locus_tag="ACHKAU_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO5533397.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4909..5874
                     /gene="dusB"
                     /locus_tag="ACHKAU_004464"
     CDS             4909..5874
                     /gene="dusB"
                     /locus_tag="ACHKAU_004464"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO5533398.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5900..6196
                     /gene="fis"
                     /locus_tag="ACHKAU_004465"
     CDS             5900..6196
                     /gene="fis"
                     /locus_tag="ACHKAU_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO5533399.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6282..7166
                     /gene="yhdJ"
                     /locus_tag="ACHKAU_004466"
     CDS             6282..7166
                     /gene="yhdJ"
                     /locus_tag="ACHKAU_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5533400.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7250..7429
                     /gene="yhdU"
                     /locus_tag="ACHKAU_004467"
     CDS             7250..7429
                     /gene="yhdU"
                     /locus_tag="ACHKAU_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO5533401.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7432..8094)
                     /gene="acrS"
                     /locus_tag="ACHKAU_004468"
     CDS             complement(7432..8094)
                     /gene="acrS"
                     /locus_tag="ACHKAU_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO5533402.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         2016 a         1973 c         2056 g         2132 t
ORIGIN      
        1 ctatttttgt tgcgattttt ggcccatgat tttcttttcg aattgtcgat attattgaag
       61 gataactttc aaagaaaaat acaaggggct atagtgttac gcattaaagg ccaatgaaga
      121 gtgttaacgt gccgagaaaa tcgtctaacc tactgaaacg cagattaaaa attgcagaaa
      181 ttgttgccgc ccaaggtgaa atcaaagttg atgacttatc tgcgcaactt ggtgtctctg
      241 gcgtgacgat ccgcggcgat ctcagttatc tggagcaaca gggatattta aagcgatcat
      301 ttggcggtgc tattgcgaca ccactctcct ctgaaccaga agcgatacaa ccgatcgcgc
      361 cattgtctct ggcacaacaa atggaaatag cacgacattg cgcacggtta attcgcgatc
      421 gtgacacgct atttcttgga catgggacaa tctgccgtaa gatcattcca ttgctcagcg
      481 aagtgaaaaa actgcgtctg atcaccaacg atcccgaaca tgcgctgctg gcaaatcagt
      541 ttattgatgg cgaaataata ctggctggca gtgaaatgct gcgccccgac accgcactgg
      601 ccggaaaacc gcttgaacag gcacttcagc atttcaccat tacacatagc attctggaaa
      661 tcagccatct ggatgctgat ggtactttaa atatcaacgt tcccagactg gccgctgcct
      721 ggcagctctg tttcgatcat gcccagaata agacggttat cgttgccgcc gatcctgcgc
      781 catcaacggc tgctatcggg catttgcatc aggcagaaaa tgtgatcgtc agccgcagca
      841 ttaacgaaca atatcagcaa caattgcagt cggctgattt cgttatcctt tataccagta
      901 atgagtgctt tacctggtcc aatcgcgaaa ggcttcagga atgagagggg ttttgtgaca
      961 ataactatct gcgctatggg cgaattgctg gccgagtttt tgtcccgcaa cccacatcaa
     1021 aaattcactc agcctgggga gtttatcggg ccatttccca gcggtgcgcc agcaattttt
     1081 gctgctcagg tggcaaaact gtcccatcgg gccatcttct tcggatgtgt tggtaatgat
     1141 gattttggcc gactcattat agagcgcctc cgtcatgaag gtgtcattac cgatgggatc
     1201 catgttatga acaatgccgt cacaggtacg gcgttcgtga gttatcaaaa tccccagcag
     1261 cgcgatttcg tctttaatat ccctaacagc gcctgcggct tgtttactgc tgagcacatt
     1321 gataaggatc tgctaaaaca gtgtaaccat ctccatattg tgggctcatc gttgttctca
     1381 tttcgcatga tcgatgtcat gcgtaaagca ataacgacga tcaaatcggc tggcggcacc
     1441 gtttctttcg atcccaatat tcgcaaagag atgctgagca ttcctgaaat ggcgcaggct
     1501 ctcgattatt tgattgaata tacggatatt ttcatcccca gcgaaagcga actccctttc
     1561 ttcgcgcgtc acaaaaatct gtcagaagaa cagattgtta gcgatcttct ccacggcggc
     1621 gtaaaacatg tggcgataaa acgcgcccag cgtggggcca gctattacaa gcttaaaaac
     1681 ggtacattac acgcccagca tgttacaggc cacgatatcg aaattatcga tccaacgggt
     1741 gcaggcgact gctttggcgc aacgtttatc actcttttct tatccggttt cccggcacat
     1801 aaggcactgc aatatgcaaa tgccagcggc gcgctcgccg taatgcggca aggtccgatg
     1861 gaagggatat cctcactggc tgacattgaa gactttttgc agcagcactg aacggcaatt
     1921 ttctgtcaga ttattttccc tgcctgaaga gttgccgtag aattgcccgt ttctcgtatc
     1981 cccccatgag gaggaaccct tccgtggaca ctcgttttgt tcaggcccat aaagaggcgc
     2041 gctgggcgct ggggctgacc cttttgtatc tggcagtttg gttagtagcc gcttacttac
     2101 ctggcgttgc ccccggtttt accggctttc cgcgctggtt tgagatggcc tgcatcctga
     2161 cgccgctgct gtttattgga ctgtgctggg cgatggtgaa atttatctat cgcgatatcc
     2221 cactggagga tgacgatgca gcttgaagta attctaccgc tggtcgccta tctggtggtg
     2281 gtgttcggta tctcggttta tgcgatgcgt aaacggagca ccggcacctt ccttaatgag
     2341 tatttcctcg gcagccgctc tatgggcggt attgtgctgg cgatgacgct caccgcgacc
     2401 tatatcagtg ccagttcgtt tatcggcggg ccaggagctg cttataaata cgggctgggc
     2461 tgggtattgc tggcgatgat tcagcttcct gcagtctggc tttcactcgg tattctcggc
     2521 aagaagtttg cgattcttgc gcgccgctac aatgcagtga cgctgaacga tatgctgttt
     2581 gcccgctacc agagtcgtct tctggtgtgg ctggcgagtt tgagtttgct ggttgcgttc
     2641 gttggtgcga tgaccgtgca gtttatcggc ggtgcgcgcc tgctggaaac cgcggcgggt
     2701 attccttatg aaaccgggct gctgattttt ggtatcagca ttgcgttata taccgccttt
     2761 ggtggctttc gcgccagcgt gctgaacgac accatgcaag ggcttgtgat gctgattggc
     2821 accgttgtgc tgcttattgg cgtggtacat gccgctggcg gcttaagcaa cgcagtacag
     2881 accttgcaaa ccatcgatcc gcaactggtt acgccacaag gcgctgacga tattctgtcg
     2941 cctgccttta tgacgtcgtt ctgggtactg gtgtgctttg gcgtgattgg cctgccgcat
     3001 actgcggtgc gctgtatctc ttataaagac agcaaagcag tacaccgggg gatcatcatc
     3061 ggtacgattg tagtcgcaat cctgatgttc ggtatgcacc tggccggagc gttaggtcgt
     3121 gcagtgatcc ccgatctcac cgtaccggat ctggtgatcc cgacgttaat ggtaaaagtg
     3181 ctgccaccgt ttgctgccgg aatcttcctg gctgcaccta tggctgcgat catgtcgacg
     3241 atcaatgccc agttgctgca aagttccgct acgatcatta aagatctcta tctgaatatc
     3301 cgtccggatc aaatgcaaaa cgagacgcgt ctgaagcgga tgtcggcggt aattacgtta
     3361 gttctcggcg cgttgctgct gcttgccgcc tggaagccgc cagaaatgat catctggctg
     3421 aatttgctgg ccttcggtgg gctggaagcc gttttcctgt ggccgctggt gctgggtctt
     3481 tactgggaac gcgccaacgc caaaggcgcg ctaagtgcga tgatcgttgg cggcgtgctg
     3541 tatgccgtac tcgcgacgct gaatattcag tacctgggct tccaccctat tgtgccctcg
     3601 ttactactaa gtttgctggc tttcctggtc ggaaaccgtt tcggtacatc cgtcccgcaa
     3661 gctaccgttt tgactactga taaataaaga gttttgccat gccttggatc caactgaaac
     3721 tgaacaccac cggcgcgaac gcggaagatc ttagcgatgc gctgatggaa gcgggtgccg
     3781 tttctatcac ttttcaggat acccacgata cgccagtatt tgagccgctg ccgggcgaaa
     3841 cgcgcctgtg gggcgacacc gatgtgattg gtctgttcga cgctgaaacc gatatgaacg
     3901 acgtggtggc gattctggaa aaccatccgc tgctcggcgc aggcttcgcg cataaaatcg
     3961 aacaactaga agataaagac tgggagcgcg aatggatgga taatttccac ccgatgcgct
     4021 ttggtgaacg actgtggatc tgccctagct ggcgtgatgt gccggacgaa aacgccgtca
     4081 acgtgatgtt agatccaggg ctggcgtttg gtacgggtac ccatccaacc acctctctgt
     4141 gcctgcaatg gctcgacagc ctcgatttaa ccggtaaaac agtcatcgac tttggctgtg
     4201 gttccggcat tctggcgatc gcggcgctga aactgggtgc agcaaaagcc attggtattg
     4261 atatcgatcc gcaggcgatt caggccagcc gcgacaacgc cgaacgtaat ggcgtttctg
     4321 accgtctgga actctactta ccgaaagatc agccagaaga aatgaaagcc gacgtggtgg
     4381 tcgctaacat ccttgcaggc ccattacgtg aactggcacc gttaatcagc gtcctgccgg
     4441 tttcaggcgg tttgctgggc ctttccggta ttctggcaag ccaggcagag agcgtttgtg
     4501 aagcttatgc cgatagcttc gcactggacc cggtcgtgga aaaagaagag tggtgccgta
     4561 ttaccggtcg taagaattaa ccttcgcatc gccgtagggt gacgcggggc gaagtgcgag
     4621 caagctcaca aaaggcacgt aaatttgccg gttatttacg caaatttgcg tgccaaaatt
     4681 ttcattcata aagaaaaatt gagaacttac tcaaatttct ttgagtgtaa attttagtca
     4741 ctattttcta atatgatgat ttttatgagt aattatcgca ccacgctcat tttaaatgca
     4801 attctttgat ccatctcaga ggattggtca aagtttggcc tttcatctcg tgcaaaaaat
     4861 gcgtaatata cgccgccttg cagtcacagt atggtcattt cttaactcat gcgcatcgga
     4921 caatatcagc tcagaaatcg cctgatcgca gcgcccatgg ctggcattac agacagacct
     4981 tttcggacgt tgtgctacga gatgggagcc ggattgacag tatccgagat gatgtcttct
     5041 aacccacagg tttgggaaag cgacaaatct cgtttacgga tggtgcacat tgatgaaccc
     5101 ggtattcgca ccgtgcaaat tgctggtagc gatccgaaag aaatggcaga tgcagcacgt
     5161 attaacgtgg aaagcggtgc ccagattatt gatatcaata tgggttgccc ggctaaaaaa
     5221 gtgaatcgca agctcgcagg ttcagccctc ttgcagtacc cggatgtcgt taaatcgatc
     5281 cttaccgagg tcgtcaatgc agtggacgtt cctgttaccc tgaagattcg caccggctgg
     5341 gcaccggaac accgtaactg cgaagagatt gcccaactgg ctgaagactg tggcattcag
     5401 gctctgacca ttcatggccg tacacgcgcc tgtttgttca atggagaagc tgagtacgac
     5461 agtattcggg cagttaagca gaaagtttcc attccggtta tcgcgaatgg cgacattact
     5521 gacccgctta aagccagagc tgtgctcgac tatacagggg cggatgccct gatgataggc
     5581 cgcgcagctc agggaagacc ctggatcttt cgggaaatcc agcattatct ggacactggg
     5641 gagttgctgc ccccgctgcc tttggcagag gttaagcgct tgctttgcgc gcacgttcgg
     5701 gaactgcatg acttttatgg tccggcaaaa gggtaccgaa ttgcacgtaa acacgtttcc
     5761 tggtatctcc aggaacacgc tccaaatgac cagtttcggc gcacattcaa cgccattgag
     5821 gatgccagcg aacagctgga ggcgttggag gcatacttcg aaaattttgc gtaaacagaa
     5881 ataaagagct gacagaacta tgttcgaaca acgcgtaaat tctgacgtac tgaccgtttc
     5941 taccgttaac tctcaggatc aggtaaccca aaaacccctg cgtgactcgg ttaaacaggc
     6001 actgaagaac tattttgctc aactgaatgg tcaggatgtg aatgacctct atgagctggt
     6061 actggctgaa gtagaacagc ccctgttgga catggtgatg caatacaccc gtggtaacca
     6121 gacccgtgct gcgctgatga tgggcatcaa ccgtggtacg ctgcgtaaaa aattgaaaaa
     6181 atacggcatg aactaattca ggttagctaa atgcttgatt aaaaaggcgc tactcggcat
     6241 ggggaagcgc cttttttata ggtgtcacaa agggagtgac catgagaaca ggatgtgaac
     6301 cgacccggtt tggtaatgaa gctaagacca ttattcacgg tgatgcgctt gccgaactta
     6361 aaaagatccc cgccgaaagt gtcgatctga tctttgccga cccaccatat aacatcggta
     6421 aaaattttga tggtctgatc gaagcctgga aagaagatct gtttatcgac tggctgtttg
     6481 aagtgattgc agagtgccac cgcgttctga aaaagcaggg cagcatgtac atcatgaaca
     6541 gcacggaaaa catgcccttt atcgatctcc agtgccgcaa gctttttacc atcaaaagtc
     6601 gcatcgtctg gtcatatgac agttctggag tacaggcgaa aaaacactac ggctccatgt
     6661 acgaacccat cctgatgatg gtgaaagacg caaagaacta cacattcaac ggtgatgcta
     6721 ttctggtaga agccaaaacc ggatcgcagc gcgcgttgat cgattatcgc aaaaatcctc
     6781 cacagccata caatcatcaa aaagtaccgg gtaacgtctg ggattttccg cgcgtgcgtt
     6841 atttgatgga tgaatatgag aaccacccga cgcaaaaacc ggaagcctta ctgaaacgca
     6901 ttattctcgc ctcttccaac ccaggcgata tcgttctcga cccgtttgcc ggtagcttta
     6961 ctaccggtgc cgtagccgtc tccagcgggc gaaaattcat tggtattgag atcaacagcg
     7021 agtacatcaa aatggggctt cgacggctgg atgtcgcgtc gcattactct gcggaagaac
     7081 tggcgaaagt gaaaaaaaga aagaccggca acctgtcaaa acgaagccgg ttaagcgaag
     7141 ttgaccccga tctcattgca aagtaaagga agtgtaagcc tggttttcag attattcatt
     7201 tcgtgtatat ttcctgccag acttggttaa acatacacag gctcaggtaa tgattcgcaa
     7261 gtattggtgg ctcgtcgttt tcgctgtctt cgtttttctg tttgatactt tactgatgca
     7321 gtggattgaa ctgctggcaa cagaaacaga caaatgccgc aatatgaact cagttaatcc
     7381 actaaaactg gttaactgtg acgaactgaa ttttcaggac agaatgtgaa tttacatgac
     7441 acttaattca ttcgtttgat gaattaattt cgttatgttt tcatctggca tgaacattct
     7501 taatacgtta tcgaccagag cgggggcttg tttataaaga tcataacccg ccatattcat
     7561 taaccagttt tgaacaattc cgctgaaggc accattaata ataatcatca caacatctaa
     7621 atcgaggtta tttgctacac aaccttgttg ctgacacgcc tgcaatactt cgcggagagt
     7681 ctgcggattg aagcccatct tttcgcgtat cactccctcg gccagcatct catcattaaa
     7741 ttcacatttg tgatataaga ttttcagcaa cgcctgctgg cggggaattt tggcaatata
     7801 ttgcaagccg acaatcaatt tttcacgcaa ttgttgaaac gggtcatgct ctaatccagc
     7861 cgtcaagtgt tcctggatta actcccgcaa tgaaggctgt tgcaaccaca tctcattaaa
     7921 cagttgagtc ttgttttcga agtgccagta gatagcgcca cgcgtaacgt tagcagcgtc
     7981 ggcaatgtcg ttgagtgtcg tcttgcttac gccatgctgc gcaaactggg cgatggcagt
     8041 ttcaatcagt tcttgccggg tcttcagagc ttcggctttg gttctttttg ccatgattaa
     8101 ttattcagga aataaatata ttcgacacag agtgagaaaa tagcgaaggt taatctatca
     8161 cctaatgtgt atttata
//
LOCUS       DAZFJF010000174         8120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFJF010000174 DAZFJF010000000
VERSION     DAZFJF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.175"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(63..206)
                     /locus_tag="ACHKAU_004469"
     CDS             complement(63..206)
                     /locus_tag="ACHKAU_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO5533403.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            complement(268..534)
                     /locus_tag="ACHKAU_004470"
     CDS             complement(268..534)
                     /locus_tag="ACHKAU_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO5533404.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFER
                     LTEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
     gene            complement(554..811)
                     /locus_tag="ACHKAU_004471"
     CDS             complement(554..811)
                     /locus_tag="ACHKAU_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="HFO5533405.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
     gene            complement(821..1018)
                     /locus_tag="ACHKAU_004472"
     CDS             complement(821..1018)
                     /locus_tag="ACHKAU_004472"
                     /inference="COORDINATES: protein motif:HMM:NF020569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="HFO5533406.1"
                     /translation="MVLTWFIFRIPSGPHKGRKIYEWDSQHGELEGYRASDGQHLGSF
                     DPKTGNQLKGPDPKRNIKKYL"
     gene            complement(1426..1569)
                     /locus_tag="ACHKAU_004473"
     CDS             complement(1426..1569)
                     /locus_tag="ACHKAU_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO5533407.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            complement(1631..1888)
                     /locus_tag="ACHKAU_004474"
     CDS             complement(1631..1888)
                     /locus_tag="ACHKAU_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO5533408.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTE
                     HPSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
     gene            complement(1891..3636)
                     /gene="col"
                     /locus_tag="ACHKAU_004475"
     CDS             complement(1891..3636)
                     /gene="col"
                     /locus_tag="ACHKAU_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="HFO5533409.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAVPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKES
                     KRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEV
                     SKDPELSKQFNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNL
                     RITTPKRHIDIHRGQ"
     gene            complement(3869..6088)
                     /gene="mobQ"
                     /locus_tag="ACHKAU_004476"
     CDS             complement(3869..6088)
                     /gene="mobQ"
                     /locus_tag="ACHKAU_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFO5533410.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKEQDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSLGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            6267..6542
                     /locus_tag="ACHKAU_004477"
     CDS             6267..6542
                     /locus_tag="ACHKAU_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFO5533411.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPKPPAKPQNH"
     gene            complement(6760..7686)
                     /locus_tag="ACHKAU_004478"
     CDS             complement(6760..7686)
                     /locus_tag="ACHKAU_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5533412.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
BASE COUNT         1964 a         2231 c         1803 g         2122 t
ORIGIN      
        1 aaaaaaaaaa gcgcatcaga atcagcctga cacgctttgt ttttgtcaaa gaggatttcg
       61 ggttactgcg ttgctaatcc ggtcacttca gctgttgatg acggagatac ggtcccgccc
      121 tgaacatccc ggatatagtt tgcctgacat gcagacagaa taatgactgc aagaagcaat
      181 aaaataatcc ctgttatttt tttcataacg actccttgtt gtgaaagaat gccagccatt
      241 catccataaa caaccggcat tcatatttca gccctgttta aatcctgact taccgttagc
      301 agctcgccat tctttaattt ccttgacaat cccttcagga ctatcttccc tgtcatcgcg
      361 aggataataa atcagatctg aaccatctgg gtgctcagtt aatcgctcaa actctctcac
      421 taatttattg tcatcctctt cagtagcacc ttcagctcta catatttttt ttacaagctc
      481 cagaaattca gcctcggtat aatcactaat acttttcttt agctccacct tcatctctcc
      541 tgagaatatt tgatcaccaa tcaccatcac gataatcaaa tgaaacaaaa tactcattac
      601 cggaaatatc aatttgatga ttaaagtatg gttgtagcaa aggtacccat tcagctataa
      661 catcaaacca accattattg atattatctt ttaaaggcat tccaagacgt tcaatgaccg
      721 agccatcatc accgaaatct tttgaatact caccaccttt aaattcctcg gtcgtcttat
      781 caaaccaatt aatatgtaat ttaagcccca taacatcctc tcaaagatac tttttgatat
      841 ttcgttttgg atcaggtcct ttcaactgat taccagtctt aggatcgaat gagccaagat
      901 gctgaccatc actggcacga tacccttcaa gttcaccgtg ctgggagtcc cattcataaa
      961 tcttacggcc tttatggggt ccgcttggaa ttcggaaaat aaaccacgtt aagactattt
     1021 tttagtcaaa taactttcag gaatcgccag aatggcggga caacagcggt tttggtgtcc
     1081 gaaatcgtac aattctgtac atctacgcaa ggaaatgtac ggaagtaagc caaatggtta
     1141 tcggacggtc ggaacatttc gtacagcctt tacgttcagt gagttagctg gcctagatgt
     1201 aaccactcgt tcaactcaat tctcttagcg tgggaaaatt tccgttttcc aagcgccccc
     1261 cccgaatggc gaacaggtca aaatgacaag ttttctccca ccggatggaa ggttctgcac
     1321 agggatttta gatcccatct cccacatgga acttttgtga catttcaaaa aaagcgcatc
     1381 agaatcagcc tgacacgctt tgtttttgtc aaagaggatt tcgggttact gcgttgctaa
     1441 tccggtcact tcagctgttg atgacggaga tacggtcccg ccctgaacat cccggatata
     1501 gtttgcctga catgcagcca gaataatgac tgcaagaagc aataaaataa tccctgttat
     1561 ttttttcata acgactcctt gttgtgaaag aatgccagtc attcatcctt aaacaactgg
     1621 cattcatatt tcagccctgt ttaaatcctg acttaccgtt agcagctcgc cattctttaa
     1681 tctctttaat aacagcttca gggctaccat cattattacc ttctgggtaa taaatcagat
     1741 cagaaccact aggatgctca gtaacactta taaaatgctc gaggttatca tcctgttttt
     1801 tttcatcacc ttcacaattg atgatgtctt caataatttt tttgaattca gtttcagtgt
     1861 aatcactaat gctgtttttt agttccattt ttactgacct ctgtgaatat caatatggcg
     1921 tttcggcgta gtgatacgga ggttgtccat atcataaaca ccaccatcct gactgattgg
     1981 cttgtcgtga tgaagctcaa atgagcgacg cccaccgaca gtatctttat tcctagcccg
     2041 tggagctaat ccttggctca agcgtttttt attacctgga ttaaattgct tacttaactc
     2101 aggatctttc gacacttctt cccagaattt tctccgaaaa tcgtcgaagt ttttaaattc
     2161 tttatcacgc aacttatcag caatgcgatc tggaattggc gctcctgaat ctttacctgc
     2221 atcatccagc catttatcac caactggttt acctttacct gtcgccttcc ctggcttatt
     2281 ccgtttactc tccttatcta atttatcctt agcgtccttt tctttatttt ccttctgttt
     2341 gcggcgctcc tgcgcggcac ttaatgcagc atcagcatct gacttctctt ttgcagcagc
     2401 atcaaatgca gcctgcttat tatttacatc cgtctgcgcc cgctgggctt taagcccggc
     2461 catttgccac attctgtgac cgccagacat tgggtcatgg gcaaatcgat caaattgttt
     2521 tatttcagct attgcatcag caagagtctt attcgctgca tcaagttcgc ttttacgcga
     2581 attataaacc tgaacagctt tagcctgtcg ctcctgattt ctggcaacat cttcatttgc
     2641 ctgattcagc tctgcacgcg cgcgttcata atttcgctca gccgcttcaa ccggatgcgt
     2701 agcatcccat tcctgctgac ggcgattttc ttcatcctga cgttgtttta cctggtcagg
     2761 gctaagaaca tcactcactg aaacatatac ggcattatga ccgctgtcct tcgggaatcg
     2821 aataactgca tccctggtat taccaccctg agtaaatcct gccgggcgaa catccttatc
     2881 agtattattt gtaacacctg ggcttaatgt ctgtactgct ggcgtactgt tattaactga
     2941 aatattcaga acaggtgcac ctggaattga tgccgtaaaa acacccggac gttcggtagg
     3001 ttttgcatca accaccggaa cactcatcgg aacacctgaa acaaccgaaa tattctgtcg
     3061 ttcgtctttt acatcatcaa caacacgaac atttacgttt actgttgcct tatcgagagg
     3121 taatgaactg acaggtgatt cagtaatatc atctgcgggt aatgacgtca caatctttga
     3181 catcatattg gggtcatctt tcgctatttg tgatggcaat acaccatata aagccacccc
     3241 ccaaagacca aatttaaacg gtcctttcag ggcagccata atatcagcaa tagctgccga
     3301 taatgctccc gctgaaatac tgaccgccag accgccagct cctggagtgg aaagtgccgg
     3361 aaaaccaaat gccactggca cagctactgc tgacagatta ccgcctgttc ccgagccacc
     3421 accggaatta ccattccccc cgccattacc atgaccggaa ccaccacccc agtgaatgcc
     3481 gctaccggaa ccaccacccc acgggttatt ttccgaactc catccggagc catcagaagc
     3541 accaccacct acaccaagcc cggtcgggcc accattaatg ttaccacttg tgctatgcgc
     3601 gcccgtgtta tggccgcgtc catcgccacc gctcataaaa attccctctt ttaagcgtta
     3661 aaacaattaa ttacatgatt aatactgtac ataaaaccac tggttttatg tacagatcaa
     3721 aaagtacccg tcatcgtttt ttctgtcaag ctcaggtgga aaatttttta gaaatcagga
     3781 gggataacac ttagaaaacg cagagcgggc cacaaaaaat tttgtggccc gtgatgtgtt
     3841 ggcggggtcc ggggtgcagc cccggacatc acatactcat gccccggaag tgcccggggc
     3901 ggcgttgttg ttgcctgtgc tgttcgtccc gcttccactc atgccaggtc tgattaaaac
     3961 cgagttgacg gaagagataa tccggcatct cttccggttt gtggacagca gccaaaagcc
     4021 tgtcatgatg gcctttgagc cggaactgca tcaggtgcgg ttccggtggg tacggagtat
     4081 cctcatcatg gtttatagct tctccgtaac ctgccaggca taccccgttc tccgtgatgg
     4141 tgctgactat cagatgactg taatccggcg acggaagcac aagaacgggg caaaaccctc
     4201 cttcccggcg aacaaacccg acaagcattt cctccggccc tttgcataac aacagattca
     4261 gcgtcctgct gtttccctct ctcgaaaata caacaggcat gagttgttcc ggtacagcat
     4321 cagcggtgat atccacccgg tgcgctgacg gcagcggaga ggctacgggc ctgcggtcgt
     4381 cgctgaaata aaaattatcc ggccagtgat gcggcagcgc cggaaaaccg taagagggcc
     4441 acccctggcg catctgttcc agtacatgcc ggaccgtcgc accggaatac attctcacac
     4501 gctcaggccg cgccatcgtc tcatgagatt ctggcattcc ctcacgtata tccgagacca
     4561 tcacagcccc gcctagcgac aggtaaacat tcaccgggtc atgaacactc tgggggtaat
     4621 gcgccagctc atcaccccat accgtgaatt ctccggctct gttactcagc atcatgtagc
     4681 gggttatcct gcccgttccc ggctctctgg cacgccccag ctccgtaata accccgacta
     4741 atgccgtttc acggctcaga cggtggttat catgacgttt cagccgcgcc tcatgctcct
     4801 gttccttccg gcgcgcttcc gcttcggctt cctccctgcg ccgctccgct tcccgtgctg
     4861 acagtttttc tttcagctct gccattcccg gcgcaatcca cgtattccac tgcgtttcaa
     4921 aggcctgacg acgggcggga atgtcctgtt ctttcacggc cctgtaggtg cggcgaagag
     4981 attcaatctg tttcccgagt ttccagaaac ggagttgtct tttgcggtat tccggttcag
     5041 cccatgtcat caggggaatc tctgacagaa gggacgcgac agggcgtgct tttttgagtc
     5101 cggtcatctc cttttcgaga atggcctgct ctttcagcag cgcattcccc tcacgttcga
     5161 tgtcgttcac cttcccgctc atcgccagga actccggggt catctcccac gcgttgccgc
     5221 attcctgcgg caacgtggca atgaattccc gcagggtatc cgctgaggca ttcgcagcgg
     5281 gaatggtcag gttaagggct tccgctgcac gacgtgcggc atcgctgagt tcccgtttcc
     5341 gcacgttctc ctgtacctgg atgactgcca gccttgtgcg ggaaacctgt atttccaggt
     5401 taaggctgtt aatcaggcgg ttctgctcac cgcgttctgt ttccattccc cgtttttcca
     5461 tcgcacaggc agcctttccg aggtgaaggc ccggcgcttt ctccagtccc tgacgctcgt
     5521 atgaacgatg gtctatctct tcctggtagc cggcgttcgc aagcgctctg ttcgcatggt
     5581 cagcccatga tgcgcgccac gtctcggcga gtgcccggtc gttccagtcc ctgacctttc
     5641 ctgcgaatcc cgtttccccg acagctctcg tggtcagcat gatgtgcgca tggggattgg
     5701 tccggtccat gtgatggaac gccacatcgg caatcatgcc ccgactgaca aagttttccc
     5761 ggacaaaagc gagaacggtc tcccgtgcgg cctcgcggga aatctcacgg ggaatggcga
     5821 gttcaatctc ccttgccagc tgggagttcc ggcgctgttc ggctttctcg accgcattcc
     5881 agagaacgga acggtcacca caccaggacg gtgcattcgc aggggtgaga atgaccgcat
     5941 catcaacgcc gcgtttacgg gtgtaatcgt ggattaatcc ggtacggtca tcgggtatga
     6001 cagagccgga acggtaggca gcagaagcaa cagcactgta gccgtttttt cgcgaaatga
     6061 ttttcatgct gagatgataa atcgccattt ctgccgtaat gcctttttgc cgttttcttt
     6121 tagcgtcgca gaccgcacac aatcgaaacg aagtggagat tgtataagtg cgcacttcgt
     6181 gatgtgccgt gcattatgcc agttcagttt tgcaactacc agtgacgcga taaaatgcga
     6241 tttttcaccg cgcacggagt cacaaaatga gcagaacact cgaacagaag attgccgaag
     6301 ctgaggccag attacagcgc ctgaaggcga agagccggag cctggacacg gcgcaaaaag
     6361 tcattgtggg ggctgccatg ctggcgaggg tacggaggcc ggaagaggcg caactgcgtg
     6421 cgttcctgct ccagtttctg cgaaaggaag tcacaagaca ggcggatgtg aaccgactac
     6481 aaccgctgat taacgagctg gagaagctac ccaaaccacc tgcgaaaccg cagaaccact
     6541 aagccccggc gcgtaagagc cacatgcacc accggacgcc tttgcgcttc ctcgctcatt
     6601 cgctcgctac gctcggtcat gagactgcgg cgagcgctac aacctcaatc atgcggaaag
     6661 ccgcgccatt gcgggattca acgagggaaa tagcaacaaa ccaagctgct caaaaaagcg
     6721 ccaaaaaggc gctgttatct gatataggtt ttatttggtc tactctacaa gacctcgttt
     6781 tttcagttgg taataccagg cgcggctaat tccaagagtc tcccacggtt tcaacgttcg
     6841 cgcagatgtg gcaacagcac cacgcttaga cttagcacct ccaattttcc cgcccttgcg
     6901 accacgtgca gcctgaattt ctggcgtgtg cgtatcagcc acatactgtg cgaaagtttc
     6961 cggcgtgaag ttcctgtgcg tgtatttcgc aatgctcttg ccaatagccc gacattccgc
     7021 cggagaaagc ggaacgggaa gcgatacgtt gtacatttcg acacgctgga tcacggcatc
     7081 aagccattgt gagaatacag gccagccctg acgaatcgcc ctgtaagccc atttgcgcgt
     7141 catttcgaac aggtggcagt ttcgccccat cccgtaatgt ttatcgatgc tacgacgctc
     7201 tgaggcgctc aaatcgagat aatcagccag ttcatcgagg gtataggctt cctcgcgcca
     7261 ctccatcacc agccattcca ggtggaacgg atttttgcaa atcagtccgc tgtagttcac
     7321 atctgcacca agtttctcac acagcgcacg ctctatcgcg gcagcgtact tcagtgcctt
     7381 aaccgacgca tcaggcgcgg ttctcacggc gatgttgagt gcatagagca ggtgagcatg
     7441 accgttcacg ggatttttta cggtgatgtt cggcgcgggg gcgttccggt cgctccagtc
     7501 aatcgcagcc ccctcgcggt caacgtcaaa aaccagccag aactgcgcat gaggctggtt
     7561 ctgctggatg taccgcgcaa gaagcgcacg ccctttgccg gagatgcgaa gaccaaaagc
     7621 gagatcatcc gtgtgatagg gacggtgggg taaattttct tcgaagtatt gaagcgcggc
     7681 gctcatgtgt tacgattcct tccaggttat cgaccgacct cgcccctgta ttctcgtgac
     7741 aatcttggcg gagagacacg aatttcaggg gcgtttgttt ttcagtcgct tacactacac
     7801 ccaacggcaa atcctgtcga accttcccga aaacccgatc catttaccat aactcccgtt
     7861 gtacgcaccc cttaaaacgc cctcagacgc gttctgacgc aaaaaacgca aaaagacagg
     7921 aaaacaccag gcaaaaccga aaaaacgcgt cagaatcgtt ttcaggggcc tgcataacta
     7981 ttcacgaaag cgatgcgcga tcggtttcgt gcgttctgaa caccgaacgg ctcaaaacga
     8041 caagttttct ccccctggat ggaaagttct gcacgaggat tttagatccc atctcccaca
     8101 tggaattttt gtgacatttc
//
LOCUS       DAZFJF010000175         8016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFJF010000175 DAZFJF010000000
VERSION     DAZFJF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.176"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..321
                     /locus_tag="ACHKAU_004479"
                     /pseudo
     CDS             1..321
                     /locus_tag="ACHKAU_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    2..66
                     /locus_tag="ACHKAU_004479"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            844..1359
                     /gene="ehxC"
                     /locus_tag="ACHKAU_004480"
                     /gene_synonym="hlyC"
     CDS             844..1359
                     /gene="ehxC"
                     /locus_tag="ACHKAU_004480"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO5533413.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            1361..4357
                     /gene="ehxA"
                     /locus_tag="ACHKAU_004481"
     CDS             1361..4357
                     /gene="ehxA"
                     /locus_tag="ACHKAU_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO5533414.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            4407..6527
                     /gene="ehxB"
                     /locus_tag="ACHKAU_004482"
                     /gene_synonym="hlyB"
     CDS             4407..6527
                     /gene="ehxB"
                     /locus_tag="ACHKAU_004482"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO5533415.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            6531..7970
                     /gene="ehxD"
                     /locus_tag="ACHKAU_004483"
     CDS             6531..7970
                     /gene="ehxD"
                     /locus_tag="ACHKAU_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO5533416.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT         2554 a         1257 c         1807 g         2398 t
ORIGIN      
        1 ctgctggata agctgcgtga gcagtacggg gtcggaccgg tatgcagtga actgcatatt
       61 gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc atcctgataa acgcagtgcc
      121 cgtgcgcagc gcgatgactg gctgaagaga gagatacagc gcgtatacga tgaaaatcat
      181 caggtatacg gtgtgcgtaa agtctggcgc cagttgttac gcgaaggtat cagggtggcc
      241 agatgtacag tggcgcgcct catggcggtt atgggacttg ccggtgttct ccggggtaaa
      301 aaggtccgta ctaccgtcag ccgatttacg ggccgtaagt aacgaagttg gatgcaaatg
      361 tcagatcgtg tgcgcctgtt atggctcggc tggtaagaga gccttatagg cgcatttgaa
      421 aaaactccag ctataccgga agatatttat tattaacagg agaggtttgc ttttctgtgt
      481 aggtttagat tttgtgtttg cagggtaaac aaaactgatt aactcgtcat ttcggtaact
      541 gaaatagttt gctgtattct gtttacaatc tctttaataa ggcatttgtc acgtggctat
      601 tcatatgaaa atcatacgtt atcttacaaa tcaatcatct gagtgttata atataactta
      661 gctgtgatat gtgtaagaat gtttaggcaa tatattatta tcgctaatgt tgttttggtt
      721 gggagagatt ctccttgcgg tagctttttg tttttagatg cttcttgctt aaaaagatat
      781 aatttctgtt cttttatata ggattcttta catttactga gttttcttat ttatttaaac
      841 attatgaaat ctaatgcttt tgacgtcatg gggaaggttg catggctatg ggcctgttct
      901 cctctgcata aaaaatggcc attatctgtt tttgctatta atgtcatacc tgctattcag
      961 acaaatcaat ttgctctctt aataaaggat gagcttcctg tagctttttg tagctgggcc
     1021 agtttagatc tggaatgtga ggtaaaatat ataaatgatg taacatcttt gtatgcaaaa
     1081 gactggatgt cgggagaaag aaagtggttt attgactgga tagctccatt tggacacaac
     1141 atggagttat ataaatacat gaggaaaaag tatccttatg agttattcag agcaataagg
     1201 ttagatgaga gcagtaaaac aggaaaaata gctgaatttc atggaggggg gatagataaa
     1261 aaactggcat caaaaatatt caggcaatac catcatgaac ttatgtctga agtaaaaaac
     1321 agacaagatt ttaattttaa tattgagaaa gaaaactaat atgacagtaa ataaaataaa
     1381 gaacattttc aataatgcga cattgactac aaaatcagca tttaatacag catcatcaag
     1441 cgtacgttcc gctggcaaaa aactcatatt attaatacct gataattatg aagctcaggg
     1501 cgtgggtatt aatgagttgg tcaaagctgc tgatgagctt ggaatagaaa tacaccgtac
     1561 tgaacgagat gatacagcga ttgcaaacca gttttttggt gcagcagaaa aagttgtagg
     1621 attaactgaa cgtggtgttg caatattcgc accacaactt gacaaacttc tgcagaagta
     1681 tcagaaagtt gggagtaaaa taggaagaac cgctgaaaat gtaggtaata atctgggaaa
     1741 agccggaaca gttctctcag cactacagaa ttttacgggg attgctttat caggcatggc
     1801 tcttgatgaa ttgctgagaa aacaacggga aggagaggat ataagtcaga atgatattgc
     1861 caaaagtagt attgaactta ttaatcagct tgtagataca gtatcaagta taaacagtac
     1921 cgttgattca ttttctgagc agcttaacca gcttggctca tttttatcca gtaaacctcg
     1981 cttaagttct gttggtggga aattacaaaa tttaccagac ctgggctccc tgggggatgg
     2041 gctggatgtt gtctccggaa ttctttctgc tgtatcagca agctttattc tgggaaacag
     2101 tgacgcacat acaggaacaa aagctgcagc gggtatcgaa ctgacaactc aggttcttgg
     2161 aaatgttggt aaagctgttt cgcaatatat tctggctcag agaatggcac aggggttatc
     2221 gacaacagct gcaagtgcgg gtctgatcac atcggctgtt atgctggcta tcagtcctct
     2281 ttctttcctg gctgctgcag ataaatttga gcgagctaag cagcttgaat catattctga
     2341 acgatttaaa aaattgaatt atgaagggga tgctttactc gcagcctttc ataaagaaac
     2401 cggagctata gatgcagccc tgacaacaat aaatactgtc ctgagttctg tatctgcggg
     2461 agttagtgca gcctccagtg catccctcat aggggccccg ataagcatgc tggtgagtgc
     2521 attaaccggt acgatatctg gcattctgga agcatcaaaa caggctatgt ttgagcacgt
     2581 tgcagagaaa ttcgctgctc ggatcaatga atgggaaaag gagcatggca aaaattattt
     2641 tgagaatggc tatgacgcaa gacatgctgc gtttttagaa gactctctgt ctttgcttgc
     2701 tgatttttct cgtcagcatg cagtagaaag agcagtcgca ataacccagc aacattggga
     2761 tgagaagatc ggtgaacttg caggcataac ccgtaatgct gatcgcagtc agagtggtaa
     2821 ggcatatatt aattatctgg aaaatggagg gcttttagag gctcaaccga aagagtttac
     2881 acaacaagtg tttgatcctc aaaaagggac catagacctt tcaacaggta atgtatcaag
     2941 tgttttgaca tttataacac caacatttac cccaggagaa gaagttagag aaagaaaaca
     3001 gagtggtaaa tatgaatata tgacatctct tattgtaaat ggtaaggata catggtctgt
     3061 aaaaggcata aaaaatcata aaggtgtata tgattattca aaattgattc agtttgttga
     3121 aaagaataac aaacactatc aggcgagaat gatttctgaa ctcggagata aagacgatgt
     3181 ggtttattct ggagcaggct catcagaagt atttgctggt gaaggtcatg ataccgtatc
     3241 ttataataag acggatgttg gtaaactaac aattgatgca acaggagcat caaaacctgg
     3301 tgagtatata gtttcaaaaa atatgtatgg tgacgtgaag gtattgcagg aagtcgttaa
     3361 ggaacaggag gtgtcagtag ggaagcgaac agagaaaata caatatcgtg attttgaatt
     3421 cagaaccggt ggaattcctt atgatgtaat agataatctt cattctgttg aagagctcat
     3481 tggcggaaaa catgatgatg aattcaaagg cggtaagttt aatgatatat tccatggcgc
     3541 agatgggaac gattatatcg aaggtaatta tggtaatgat cgactatacg gcgatgatgg
     3601 ggatgattat atatccggag gacagggaga cgaccagtta tttggtggta gtggaaacga
     3661 taaattgagt ggaggggatg gtaataatta tctgacagga ggaagcggta atgatgagct
     3721 tcaggcacac ggagcttata atattctgtc aggtggtact ggtgatgata aactttatgg
     3781 tggtggtggt attgatcttc tggatggagg ggaaggtaat gactatctga atggtggttt
     3841 tggtaatgat atttatgttt ataggcaaaa ctatggtcat catacaattg cagatgaagg
     3901 aggtaaagga gatcgtttgc acttatctga tattagcttt gatgatatcg catttaagag
     3961 agttggaaat gatcttatca tgaataaagc cattaatggt gtactttcat ttaatgagtc
     4021 aaatgatgtc aatgggataa catttaaaaa ctggtttgcg aaagatgcct caggagcaga
     4081 taatcatctt gttgaggtta taacagataa agatggtcga gagataaaag ttgataaaat
     4141 acctcataat aataatgaac ggtcaggtta tataaaagcc agtaatatag catctgaaaa
     4201 aaacatggtt aatatcacca gtgttgccaa tgatattaat aagattattt cttcagtttc
     4261 agggttcgat tcaggtgatg aacgattagc atctttatat aatttatcct tacatcaaaa
     4321 caacacacac tcaacaactt taacgacaac tgtctgagac taaaagagga ggtgatggcc
     4381 attccaggag gaatggtcat cgttatatga tgagtaaatg tagttctcat aatagtctgt
     4441 atgcactgat attgcttgca caatatcata atataactgt caatgctgaa actataaggc
     4501 atcagtataa tacccacaca caagattttg gggtgactga atggttactg gcagcgaaat
     4561 ccattggctt aaaagcaaaa tatgtagaaa aacatttttc cagattgtca ataatttctt
     4621 tacctgcgtt gatatggcgg gatgacggta agcattatat attgtctcgt gttactaaag
     4681 attcatcacg ctatcttgtt tatgatccag aacaacatca gtcactaact tttagtcggg
     4741 atgagtttga aaaactgtat cagggaaaag tcattctggt tacgtcaaga gcaacagtag
     4801 tcggagagtt agctaaattt gatttttctt ggtttatccc ctctgttgtg aaatacagga
     4861 ggattttact tgaggtgtta actgtttctg cttttattca gtttcttgcg ttaataacac
     4921 ctcttttttt tcaggttgta atggataagg ttttagttca ccgggggttt tcaacgttaa
     4981 atattatcac aatagcattt attatagtga tactttttga agtgatatta accggagcca
     5041 gaacttatat tttctctcat actacaagtc gtattgacgt cgaactgagc gctaagttat
     5101 tcagacattt gcttgcattg cctgtttcat attttgaaaa tcgcagggtc ggagagaccg
     5161 ttgccagagt aagggaactg gagcaaattc gtaatttttt aaccggacaa gcgttgacat
     5221 cagttcttga tctatttttt tctgtaatat ttttttgtgt catgtggtat tacagccctc
     5281 aattaacact ggttatatta ttgtcactac cttgttatgt tatatggtca ttgtttatat
     5341 cacccttatt acgtcgacgt cttgatgata agtttctcag gaatgcagaa aatcaagctt
     5401 ttcttgtcga aacggtaaca gcaataaata caattaaatc catggcagta tcaccacaga
     5461 tgattgctac atgggataaa caactggccg ggtatgttgc ttccagtttc agggtaaatc
     5521 tggttgcaat gacagggcag caggggatac agctgataca gaaaagtgta atggtaatta
     5581 gtctatggat gggagcacat cttgtcatat cgggagagat aagtatcggg cagttaatcg
     5641 cgtttaatat gcttgcaggt caggtcattg ctcctgttat cagactggct catctttggc
     5701 aggattttca gcaggttggt atttctgttg agcgccttgg tgatgtatta aatacaccgg
     5761 tagagaaaaa gtcaggcaga aatatactgc cggaaattca gggggatatc gaatttaaaa
     5821 atgtcagatt tcggtattct tctgacggta atgttatttt gaataatatt aatttataca
     5881 tatcaaaggg ggatgttatc ggtatagttg gccgttctgg ttcaggaaaa agtacattaa
     5941 caaaactgct tcagcgcttt tatataccag agaccggaca gattttaatt gatgggcatg
     6001 atttatcact tgcagatcca gaatggttac gacgccagat tggtgttgta ttgcaggaaa
     6061 atatactatt aaatcgtagt attatcgata atattacatt agcttctccg gctgtatcta
     6121 tggaacaggc tattgaggca gccagacttg caggtgccca tgattttatt acagaactaa
     6181 aagaagggta caatactatt gttggagaac agggcgttgg tctttcagga gggcagcgcc
     6241 aacggatcgc aatagcccgg gctcttgtta caaatcctcg aattcttatt tttgatgaag
     6301 caaccagtgc tcttgattat gagtcggaaa atataataat gaaaaatatg tcaagaatat
     6361 gtaagaacag aaccgtaatt attattgcac acaggttgtc aactgtaaaa aatgcaaaca
     6421 gaataattgt tatggataat ggctttattt ctgaagatgg cacacataaa gagcttatct
     6481 ccaaaaaaga cagtttatat gcatatttat atcagttgca ggcataacgt atgcgctttt
     6541 acatgaaagg tttatgggat ttggtttgcc gttataagac ggttttttct gatgtctgga
     6601 aaattcgtca cacacttgat gctcctgtga gagaaaaaga tgagtatgca tttcttcctg
     6661 ctcaccttga actcattgaa acaccagtat ccagacgttc tcattttgtt gtatggagta
     6721 ttttattatt tgtaattata tctcttctct tatctgttct tgggaaagtt gaagtggttt
     6781 cagtagcaaa tggtaagttt actcatagtg gaagaagcaa agaaataaaa ccgatagaga
     6841 atgcgattgt agaaaaaata atggtaaaag atggctcttt tgtaaaaaaa aatgatccat
     6901 tagttgaatt gacggtgcct ggtgttgaat ctgatatttt aaaatcagaa gcatctttgt
     6961 tgtatgaaaa aacagaacaa tatcgctacg caattctttc tgaatcaata caacggaatg
     7021 agcttcctga aataaggata acagattttc ctggcggaga agataacgcg ggaggtgaac
     7081 attttcagag ggttagctca ttaataaaag agcaattcat gacatggcag aacagaaaaa
     7141 atcaaaagca gctcacatta aataaaaaaa tagttgaacg ggatgcagcg cttgctcgtg
     7201 ttagcctgta tgagcatcag gtatcacagg aaggaagaaa actcaatgat tttaagtatt
     7261 tgttgaataa aaaagctgtt tctcaacatt cagttatgga gcaggaaaat agctatattc
     7321 aggcaaaaaa tgaacatgca gtctggcttg cacaggtttc tcaacttgaa aaagaaatag
     7381 aacttgtgcg ggaagaactg gcactggaga cgaatatctt cagaagtgaa attatcgaga
     7441 agcacagaaa atcaacagat aacattgtgt tgctggagca tgagcttgaa aaaaacaggc
     7501 agagaaaagc atcatctttt attaaagctc ctgtgagtgg tactgttcag gagttaaata
     7561 tacatacaga aggtggtgtt gtcacaacag cagaaacgct gatgattatt gtccctgata
     7621 atgatattct cgaagtaaca gcatctgtac ttaacaagga tatcggtttt atacaacctg
     7681 gacaggaggt cgttattaaa gtagatgcat atccatatac acgtcatggt taccttacag
     7741 ggaaagtaaa aaatataact gcagattctg tttctgttcc ggatacaggg cttgtattta
     7801 acgtgattat atctgttgat cggaatgata tacagggaga aagaaaaaaa atccctgtta
     7861 cagctggaat gactgttatg gcagaaataa aaaccggggt gcgtagtgtt atcagctatc
     7921 ttcttagtcc attaaaggaa actattaatg aaagtttacg tgaacgttag tatataaata
     7981 acatattggc tttttatatg atatagattt atgaaa
//
LOCUS       DAZFJF010000176         8010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFJF010000176 DAZFJF010000000
VERSION     DAZFJF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.177"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            107..843
                     /locus_tag="ACHKAU_004484"
                     /pseudo
     CDS             107..843
                     /locus_tag="ACHKAU_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1022..1318
                     /locus_tag="ACHKAU_004485"
     CDS             1022..1318
                     /locus_tag="ACHKAU_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5533417.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1626..1742
                     /locus_tag="ACHKAU_004486"
     CDS             1626..1742
                     /locus_tag="ACHKAU_004486"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533418.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1961..3394
                     /locus_tag="ACHKAU_004487"
     CDS             1961..3394
                     /locus_tag="ACHKAU_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5533419.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVVPNFDL"
     gene            4215..4778
                     /locus_tag="ACHKAU_004488"
     CDS             4215..4778
                     /locus_tag="ACHKAU_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5533420.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            4933..7293
                     /locus_tag="ACHKAU_004489"
     CDS             4933..7293
                     /locus_tag="ACHKAU_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5533421.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(7455..7631)
                     /locus_tag="ACHKAU_004490"
     CDS             complement(7455..7631)
                     /locus_tag="ACHKAU_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533422.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(7769..8010)
                     /locus_tag="ACHKAU_004491"
                     /pseudo
     CDS             complement(7769..8010)
                     /locus_tag="ACHKAU_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         2232 a         1586 c         1912 g         2280 t
ORIGIN      
        1 tctgaacagg gtaatagtac acgtattaac agattatgtc agaaggcccc ggtgatgcct
       61 tctgacgata gattaatgtt gtattctatt ggtatctgga gaccatatgg caacagttac
      121 agtatattct ccccgctgta attcctataa agtgtaccgt tatgatcaca gcacctcaga
      181 tcatgagcgt ttccagtgct aatcctgcaa acgtgtattt caactgaatt acatctacga
      241 aacccgaaaa ccaggcatta aagagcaaat tgttgaaatg gttcataacg gagccggggt
      301 tcgcgatagc tccagaacgc tgaaggtgga tatcaacacc gtcattctca ctttaaaaaa
      361 cgctcaccac gtaaagtaac catatctcca gccgccagtg tcgatgtggc acttatctgt
      421 gaacttgatg aacaatggag ttttgtcgaa aacaaagctc gtcagcagtg gcactggtac
      481 gcgtataaga ccaaagctga cggtgtgctg gcttacactt ttggtcctcg cactgatgaa
      541 acatgccgtg agttgccgga attcctgaag ccattctccg caggcatgat aaccagagac
      601 aaccggagca gttataccag agaaatgcca caggataagc atcttgtcgg caagatattt
      661 acccggcgca ttgagcgcaa caatctgaca cttcgtactc acatcaaacg acctgcccgg
      721 aaaacgattt gctttttacg ctccctggaa attcacgaaa agccattggt tcatttatcg
      781 aaaacacatg tactactgac aggagtcatt accagggcct cttttgctgt ttttttgcca
      841 tgaatgataa tgagaatcga cagcgataag ggaaaagtaa aataacacta atgctgtcgt
      901 tttctatcat attgattttt aattatttat tgtctttttt ctcaacccat ttttttacgc
      961 agacaaattt acccggggaa tacaaactgt aacgacagtt caccacacta tgggggataa
     1021 aatgccagat caatccggac actgcaggac tccttcacgc atcttacgga tgcctgaact
     1081 gagccaattg ttaggtatat ctcgttcgac aatttacgaa aaaatgaatc cgttatcgaa
     1141 atactacgat gcaaccttcc ccaggccggt acgtcttggt agtggttctg tgggctggcg
     1201 ttcatcagct attgacgaat ggcttacttt acatactgta cctgccagat cagcagtcaa
     1261 aggtgttaat gacgaagtga gacatacgct ttcttcagat atgaccatga gcagataagt
     1321 ttttgtcatc acgtgatcac ctcaggagga aaaagaagca taagaacaat ttagccagaa
     1381 gtttaattga taaacagaat ttccagcatt cattgttgaa agagcagcta ttgtcgctat
     1441 tgtagcggta attgctgcta taaccgaaac gtattacgaa aaaggaagca tttatgcctt
     1501 acatcaaacc ggatatgtcg tattcagtat atgcatgacc atcaatgata gggaaggcaa
     1561 taattaaagc tcaatacaat aaacaagtac ctctgactct ggttgcaatg tcagtattaa
     1621 cagcgatgtc aattgcctgt cagaatcagg ttgacgtatg cagtcccgga aatttacgtg
     1681 gggccgttaa tatttacact atggttctgg agaaagggaa aagaccgggg gaaaggtatt
     1741 agtgaaataa tttccggagg tattaagaat gagaaatatg aatgatattt cgtggtgaat
     1801 aatacagtct tcactgaatt aaccttatcg gtttattaaa catcagatct acaaatgctc
     1861 tgtatttctc agagcatcag gtctgaatag cagctatttt cgctattgta gcgggatttg
     1921 ctgctatttt actaacccat taatgaaaag agaggctttt atgtgcttac ttgcaccaga
     1981 aaatccttat ccgatatatg cactaccacc gctggtgaga aatgcaataa ttgaaactca
     2041 aaaaaataca caggcacctt tggctatggt ggcaacatcc gcattaacag cgatctcaat
     2101 tgcctgtcag aatcagattg acgtgtgcag acctggaaat ttacatgggc ctgttaatct
     2161 ttactctctg attctggctg attccggtga aaggaaaacg accgtggata aagtgtttat
     2221 gaaagcattt tatctcaggg atgaagccct ggcggatgaa tacgcgaaac tggttgagaa
     2281 ttacagtaca gaaaaggaaa tatgggagca aaaacaaaaa gcgctggaat caaaatttca
     2341 taaagagatt cgtgccggta aagattacaa ggcaacagaa tcagagcttg aaacgcatct
     2401 gaataaacct cctgttccgc cgcagatacg tcgaacaatt tttaatgaga caacgataga
     2461 gggaatgtta aaatattact ccgatagcaa tcgctctttt gctcttgtat ccagtgaagg
     2521 gggggtaatt tttgacagcc gggccatgag taaactggga attattaaca ctttgtggga
     2581 tggaggttct cttttcatcg acaggaaatc atctcccgga attaatttga aggagccaag
     2641 actgacgata tcggtgatga ttcagcctga tgtttaccac aaaggttttt gtacgcgaaa
     2701 aaaagaaatt gtgaaaacgt caggacatca tgcaaggttt ttgatgtgtc aaccaacatc
     2761 aacgcagggg acaaggataa taaccggaga taattattca tcacagtatc aggatttgtt
     2821 tgaagagagg atcaatgagc ttattgatga aagtctggcg atgagtggtg aacgacgttg
     2881 ccttcacttt tctcctcagg ctgccagaat ctggacggat tattacaatg atgtggaatc
     2941 gaagctgggg ggattggggc ctttaagaca ttgccgggaa tatgctgcta aaaatgcaga
     3001 gtatatggca agactggcag gacttattta ccattcgagc ggtgaagagg gggagatttc
     3061 cccttacact gcagaaatgg cgagagaatt agcaatatgg tacggtaatg agtatgtgcg
     3121 gttgtctaat ccgttaactt ttgacaactc tgccctgacc gtacctgtgc gactcattcc
     3181 agaggagctt gaacttttca actggataaa aagctattgt attgagaagg ggatcctctg
     3241 tatgaaaaaa aatgacattt tacagcgtgg tccgaaccgt ttccggaaaa aggataaaat
     3301 caactggtta ctggatttac tgtatgaaca aaacagagtt gtaccggtta ttgagggaaa
     3361 aacgttgtgt gtcgtaccta actttgacct ctgaacgact taatcgttga gtacatatag
     3421 tctcctctgc tacttgcgga ggagactaat gtctttacca tgtcagcgtt gtattagaaa
     3481 actctggtac aggtcagtca gtaaatataa ctggtgtaac cactatttac tattggatag
     3541 agtgttactc aggatattgt cggctttagt ctgaggtgag gtggggcctt cattgttttc
     3601 gtgttcaata aataatttag gttaattagt attattagtg gaagttatgt gatctgttat
     3661 gttgtcttgt acgcacttaa cactggttac gtaccggaac actattagag tatgttatgg
     3721 ctctgttaca gtttataggt atgaaaaaat ataatctttc ctgtatgaaa taatattctt
     3781 atggtgtgtg gggtatggtc tgtataccat catttttatg gtgaagttaa atatctgtag
     3841 aggcctcaag ctgggtcgct gtttttactt aatggtacag aagtattaat agcggtgttg
     3901 cctgtggcta cccgatactg gcttttgtga atgtcagatt acttcctgta agtgattgaa
     3961 tggtacttcc ttaaactcaa ttctatgagg tgtgttttta ccattgtaat tcgtactggt
     4021 cctcagttta agtgagtctt actgtactct gaaatattac ctctttggag taatgaggta
     4081 atacgaaaca gttttatcag gttgtctgtg ctgctggtta cctcgtttat taatgccgcg
     4141 gtacggcgtt tctgtactct cagtatttat gtaattccac cttcctgtaa cttttataaa
     4201 gtaaggacta taaaatgtat aatgctaatc ccaattacga aatggacttc atgattctga
     4261 aagacgtgaa tgaacacatg gaaggtctgt ttcaacgttt ttctaaactg ttacctttca
     4321 gaattgattt tgcctacagg aaagacacgc ccagttttgg tcattcctgt aggcacagta
     4381 tgtgtattga aatgcaccgc ttacttagtg aaacccaaac gatgctggcg ggatattact
     4441 gggtaatgga atatacgtca aacaagggac ttcatattca ttttatcggc tacctggatg
     4501 ggcaacgtca caacaaatct tatcggatat ccaggcaact gggagatatc tggaggcgga
     4561 tcacggaagg ggagggttat tttcacctgt gcagggctaa agacaaatac ccggtgcgta
     4621 tcgatcacgt aattcactat agtgataaat ctgcggtaga cgacttacgt tacgccctta
     4681 gctatctggc caaacaggat cagaaagaac acggtattat tctgaggcgt agcaggcttc
     4741 ccgaaaaaag taaccggggc cgaccacgac ataactgaat tacagctgta ttaggtatta
     4801 gaattaagtt tctgtaagtt gttgggaggt gcttgagtta ataggcagaa gagggcgggg
     4861 cgctttgtca tcagtgtagg ggacccgaat ttctgccgtg gtgcctcagt ggatcggata
     4921 caggtaacga atatggttca taaatctgac agtgatgaat tagctgcatt gagggcagaa
     4981 aatgcccgtc tggtctcatt acttgaagct catgggattg aatggcgacg taaaccgcag
     5041 actcctgtgc agcgcgtttc tgtattatcc accgacgaga aggttgcatt atttcgtcgg
     5101 ttgtttcgtg ggcgtgatga tgtatgggca cttagatggg aaagtaaaac cagcggcaaa
     5161 tcagggtact ctcctgcctg cgctaacgaa tggcaggcga gaatatgcgg taaaccccgg
     5221 ataaaatgtg gggactgcgc tcaccgtcag ttgattcctg tatccgatct cgtcatctac
     5281 caccatctgg ccggtaccca tactgtcggg atgtatccgt tgctggaaga tgattcctgt
     5341 tattttctgg cagttgattt tgatgaagct gaatggcaga aggatgcatc cgcattcatg
     5401 cgatcctgtg atgagctggg tgtacctgct gcgctggaaa tatcccgttc acgtcagggg
     5461 gcgcatgtct ggatattttt tgcctcacga gtttcggccc gcgaagctcg ccgtctgggg
     5521 actgctatta ttagctatac gtgtagtcgg acccgacagc tgcgattggg gtcttatgac
     5581 cgattatttc ctaatcagga tactatgcca aaagggggat ttggtaatct catagcgtta
     5641 cctctgcaaa aaagaccgcg tgaattgggg ggaagcgttt ttgttgatat gaatctccag
     5701 ccttatcctg atcagtgggc ttttcttgta tcggtgaccc cgatgaatgt gcaggatatt
     5761 gaaccgacga tattacgggc tacagggagt atccatcctc tggatgtgaa ttttatcaac
     5821 gaagaagacc tgggtacgcc gtgggaagag aaaaaatcat caggaaacag actgaatatt
     5881 tctattgcag aaccgctgaa aatcacgctg gcaaaccaga tctatttcga aaaagcgcaa
     5941 ttacctcagg tgctgattaa ccgacttatt cggctggcag catttccgaa ccctgagttt
     6001 tataaggctc aggcaatgcg tatgtcagtc tggaataagc cccgtgttat aggttgtgcg
     6061 gagaattacc cgcaacacat tgcgttgccc cggggatgtc tggacagcgt attatctttc
     6121 cttagggaca acaatattgc tgcagaatta atcgataaac gatttgccgg gacggaatgt
     6181 aatgccgttt ttatgggaaa cctcagagcg gagcaggaag aggccgtttc ggcattactc
     6241 cgttatgaca ctggtgtgct ttgtgcgcca acggcttttg gtaagacagt taccgcagcg
     6301 gcagtgattg ccaagcggaa agtgaataca ctgatactgg tacaccggac tgaattgctg
     6361 aagcagtggc aggagcgtct cgcggtgttt ctgcaggccg gtgacagtat tggtattatc
     6421 gggggaggta aacataaacc ctgtggcaat attgatattg cggtggtgca gtccatatcc
     6481 agacaaggag aagttgaacc tctggtcagg aattatgggc aaatcattgt ggatgagtgc
     6541 catcatattg gcgcggtttc attttctgcg attctgaagg aaacgaatgc cagatatctg
     6601 cttggcctga cggcaacacc aatccgacgg gatggtctgc atcccattat ttttatgtac
     6661 tgtggtgcca ttcgccatac agcggtccgc ccgaaggaaa gcccacataa tctggaggta
     6721 ctgatccgtt cccgttttac atctggtcat ttaccatcgg atgcgagaat ccaggatatt
     6781 ttcagagaaa ttgctctgga tcatgacaga acggtggcga tagctgaaga agccatgaaa
     6841 gctttcgggc aggggcgaaa agttctggta ctgactgaac gtacagatca tctggatgag
     6901 atagcatcag tgatgaattc actgaaattg tctcccttta ttctccatgg tcgactatcg
     6961 aaaaaaaagc gtgcgatgct gatatccggg ctgaatgctc ttcctcccga ttctcctcga
     7021 attttgttgt caacaggcag acttattggt gagggatttg accaccctcc gctggatacg
     7081 ctgattcttg ccatgcctgt gtcatggaaa gggacattac agcagtatgc agggcgtctt
     7141 cacagagagc ataccggtaa aagcgatgtc aggatcattg attttgtgga taccgcgtat
     7201 cctgtgctgc tcagaatgtg ggataaacgt cagcggggtt ataaagcgat ggggtacagg
     7261 attatagctg acggcgatga atcagtgatt taattttaag gagtacttta ccctttttaa
     7321 ataatttgag ctccccgggt tgttcgggca acaaagagtc ccagtataca ggcttcagga
     7381 atatgttgta gtatcaggct tttcatcgct ttaaaatgac taccggcagt gagaacgtca
     7441 tcaaaaataa caatctaggg cctgcaccct tccagtaatt gttcatccag ttggtagttt
     7501 gccataatcg cttctggtaa gggccggttt gttgcggtat gagaggctat tgttgatacg
     7561 ttttgcacaa ccagttccca gaaatccagc catggttcgt aggtttgcgc aattcgcaga
     7621 atttctgtca tacggtcatc gtaagcggca tctgtacggc atttggatgg tggtacggga
     7681 acaaatgtgt attcctcaag attaccgata tttgatatga gcatctgagc aacatactca
     7741 atagcagcct ttttatagtg gtaatcaggc tacgaaagta cagcgtttta taaattgtct
     7801 caggtgatat tcgcagcgtt ttttgacgtg gttttgttcg ccttaaccat cctgatattt
     7861 gctctggaga ccatttcatc tccagctttt ccagaacaag ctttcgcaat ggtaaatttt
     7921 gatccagtaa gcacggtttt ggccttttcg ccattctgtt ggctcggtta ttagcatcaa
     7981 cagctttgta atagcgtctg ccccgattac
//
LOCUS       DAZFJF010000177         7970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFJF010000177 DAZFJF010000000
VERSION     DAZFJF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.178"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..621
                     /locus_tag="ACHKAU_004492"
     CDS             <1..621
                     /locus_tag="ACHKAU_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5533423.1"
                     /translation="KDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKT
                     LDVCHYDSERKVLTSHHGIIRDEENACYCLGALQVPFKQMKNPTDPEELANIFNKKHK
                     EHMNDPLLHLIRSNIMKPTAVYADYINNTTDFRPKPERIFICGETLKCEDPSIYIRQK
                     YGHHGVGVDQNQQFDNGVMGLNSTKKVLDKNNKIIYSSGLSCFQSH"
     gene            883..2505
                     /locus_tag="ACHKAU_004493"
     CDS             883..2505
                     /locus_tag="ACHKAU_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5533424.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2695..2808
                     /gene="yhiY"
                     /locus_tag="ACHKAU_004494"
     CDS             2695..2808
                     /gene="yhiY"
                     /locus_tag="ACHKAU_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO5533425.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2871..3938
                     /gene="yhiI"
                     /locus_tag="ACHKAU_004495"
     CDS             2871..3938
                     /gene="yhiI"
                     /locus_tag="ACHKAU_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5533426.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3935..6670
                     /gene="rbbA"
                     /locus_tag="ACHKAU_004496"
     CDS             3935..6670
                     /gene="rbbA"
                     /locus_tag="ACHKAU_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO5533427.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6670..7794
                     /gene="yhhJ"
                     /locus_tag="ACHKAU_004497"
     CDS             6670..7794
                     /gene="yhhJ"
                     /locus_tag="ACHKAU_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5533428.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         2028 a         1856 c         2091 g         1995 t
ORIGIN      
        1 aaagatagcc gtattaataa aaatgtcgtc gttctggctg gaaatcatga aatcaatttt
       61 aatggtaact atactgcacg actagcaaat cataaactct ctgccggtga tacgtataac
      121 ttaattaaaa cgctcgatgt ttgtcattat gactcagaaa ggaaagtttt aaccagtcat
      181 cacggaataa taagagatga agaaaatgca tgttattgcc tcggtgcttt gcaggttcca
      241 tttaaacaga tgaaaaatcc tactgatcct gaggaattag caaatatatt taataaaaaa
      301 cataaagaac atatgaatga tcctctatta catctaatcc gaagcaatat tatgaaaccc
      361 acagctgtat atgctgatta tattaataac actactgatt tcagaccaaa acctgaacgt
      421 atcttcatat gtggagagac actcaaatgt gaagatccca gcatatacat tagacaaaaa
      481 tatggccatc atggggtcgg ggtagatcag aatcaacaat ttgataatgg tgttatgggg
      541 ctaaattcca cgaagaaagt acttgataaa aataacaaaa taatttattc ctcagggctt
      601 agttgttttc aatctcatta aaactccatg aatacggtgg tatgcctttt taatcctgcc
      661 agtatttcat aggttttctt aatcgattgt agaggtatgt actgatgacg cctctacaat
      721 ctttagggac tttctctgct atgagataag aaatttcata ttatgcttta aggctgaatg
      781 atttgtgcaa aatataatta tttttccatc gaacatgagg tgtatgtcat aagattaaat
      841 gttaactatg cattcctcgc ggtgaattat atgaggtaaa agatgaaaat tggaactgtg
      901 gcaggcacta acgacagtac aacgacaatc gcaacaaatg atatggtgca agagcatgtt
      961 actaacttca ctaaagaact gtttgggtat attgcaaatg gtataggtga tgatatatca
     1021 agtattgcca ggacaatgct cggtgaagtt gtggaaaaaa ttgatgattg gcaaatagaa
     1081 cgtttccaac aatctattcg agatgacaaa atttcattta ccatacaaac gaatcattca
     1141 gaaaaatata gtatgctatc gggcatgcgc gctcatattc tacgtaggga taataattat
     1201 cagtttattg tgacaattaa tagtataaat tacggctgct atcttgataa taccgatatt
     1261 aattggtgca gcattgttta tttacttaat aatatgacag taaatgatga tgctaatgat
     1321 gttgcagtta ctgagtcgta caaacccatc tggaactgga aaatatcgca atacaatgta
     1381 tttgatatta aattcgaaac aatgataaaa ccccagttcg ctgacagaac ctatttctcc
     1441 aattgttcac ctgttgatcc cacaagtacc agaccaacct attttggcga taccgatggc
     1501 tcagtcggtg cagttttgta cgcgcttttt gcaacgggac atttgggaat catggctgaa
     1561 ggagaaaatt tccttagcca gttgcttaat atagaagatg aagttctcaa cgtattgcta
     1621 agggagaact ttaatgagca acttgacact aatgttaata taataatcag tatcctgaac
     1681 agaagagaca ttgttttaga aagtcttcag ccttatttgg ttattaataa agacgcagtc
     1741 acgccatgta cgtttctggg cgaccagact ggcgatcgat ttagtaatat ctgtggcgat
     1801 aaatttatca tcgatttatt aaaacgcata atgagtatca atgaaaacgt tcatgtatta
     1861 gctggaaatc atgaaacgaa ttgcaatggt aactatatgc agaacttcac gcgcatgaaa
     1921 ccacttgatg aggataccta cgctgggata aaagattatc ctgtatgttt ctatggcccc
     1981 aaatacaaaa taatggcaaa tcatcatggg ataacttttg atgatcagcg aaaacgttac
     2041 attataggac cgataacagt ctctagtgat gaaatgacca atgctctcga tccggtcgaa
     2101 ttagcggcaa ttatcaataa aaagcaccat gctgtaatta atggcaaaaa gtttaaaaat
     2161 agccgggcta tctcttgtcg atcctttaat cgttattttt ctgtttcgac agattacaga
     2221 cctaaacttg aagcactatt agcatgctca caaatgttag gtataaatca agttgttgca
     2281 cataacggta atggtggaag agaatgtata ggtgaaacgg ggacagtttt aggtcttaac
     2341 gcccgtgaca gtaaacatgc tggaagaatg ttcagcatgc ataattgcca aattaaccct
     2401 ggtgcagggc ctgagataac cactccctgg aagtcttacc agcatgaaaa aaacagaaat
     2461 ggacttatgc ctttgattag aaggcgcaca atgttacagc tctgaaccat agacaacatg
     2521 aatattaatg ttactatctg gttgagactg ttgataaaac gtaaaaagga atgctctgtt
     2581 cctgcaactg aacaggcgtg aaacgcgatc caacaggatg gataccgttt tgtcagccag
     2641 ttttatagat cacacttatg agtatgcgtg tttcatttac ggccaggaat gctgatgatg
     2701 acaacattac tacccgtttt cactaagcct tccccgttgg cgctcaatgc tctgcgcgct
     2761 ggtcgtattt gtcgtttcct tcttatcccg gatggaagaa tccgctgaac gtcgttcggc
     2821 ggcagttgta acacgcgtcg tttgcttaca gggattatgg cgggccgatt atggataaga
     2881 gtaagcgcca tctggcgtgg tgggttgtcg gggcactggc ggtggctgcc gtcgtggcgt
     2941 ggtggctgtt gcgcccggcg ggtgtgccgg aaggctttgc tgtcagcaat gggcgcattg
     3001 aagcgacgga agtggatatc gccagcaaaa ttgccgggcg tatcgacacc attctggtga
     3061 aagaaggcca gtttgttcgc gaaggtgaag tgctggcgaa gatggatact cgcgtgttgc
     3121 aggaacagcg actggaagcc atcgcgcaaa tcaaagaggc acaaagcgcc gttgccgccg
     3181 cgcaggcttt gctggagcaa cgacaaagcg aaagtcgcgc cgcgcagtcg ctggttaatc
     3241 aacgccaggc agaactggac tccgtagcaa aacgtcatat gcgttcccgt tcactggccc
     3301 aacgaggggc tatttctgcg caacagctgg atgacgaccg cgccgccgct gagagcgccc
     3361 gagctgcgct ggaatcggca aaagctcagg tttctgcttc taaagccgct atagaggcgg
     3421 cacgtaccaa tatcattcag gcgcaaaccc gcgtcgaagc ggcacaagcc actgaacggc
     3481 gcattgccgc agatatcgat gacagcgaac tgaaagcccc gcgtgacgga cgcgtgcagt
     3541 atcgggttgc cgagccaggg gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg
     3601 atctcagcga cgtctatatg accttcttcc tgccaaccga acaggcgggc acgctgaaac
     3661 tgggtggtga agcccggctg atcctcgatg ccgcgccaga tctgcgtatt ccggcgacca
     3721 tcagttttgt cgccagtgtc gcccagttca cgccaaaaac cgtcgaaacc agcgatgaac
     3781 ggctgaaact gatgttccgc gtcaaagcgc gtatcccacc ggaattactc cagcagcatc
     3841 tggaatatgt caaaaccggt ttaccgggcg tagcgtgggt gcgggtgaat gaagaacttc
     3901 cgtggcctga cgacctcgtg gtgaggttgc cgcaatgacg catctggaac tggttcccgt
     3961 cccgcctgtc gcgcaactgg cgggcgtgag ccagcattat ggaaaaaccg ttgcgctgaa
     4021 caatattact ctcgatattc cggcccgctg tatggtcggg ctgattggtc cggatggcgt
     4081 cgggaagtcg agcttattgt cgttgatttc cggtgcccgc gtcattgagc agggcaatgt
     4141 gatggtgctg ggcggcgata tgcgcgaccc gaagcatcgc cgcgacgtct gcccgcgcat
     4201 cgcctggatg ccgcaggggc tgggcaaaaa tctctaccac accttgtcgg tgtatgaaaa
     4261 cgtcgatttt ttcgctcgcc tgttcggtca cgacaaagcg gagcgggaag tgcgaattaa
     4321 tgagctgctg accagcaccg ggttagcacc gtttcgcgat cgtccggcag ggaaactctc
     4381 cggcgggatg aagcaaaaac tggggctgtg ctgtgcgtta atccacgacc cggaactgtt
     4441 gattcttgat gagccaacaa cgggggttga ctcgctctcc cgcgcccagt tctgggatct
     4501 gatcgacagt attcgccagc ggcagagcaa tatgagcgtg ctggttgcca ccgcctatat
     4561 ggaagaggcc gaacgcttcg actggttggt ggcgatgaat gccggagaag tgctggcaac
     4621 cggcagcgcc gaagagttgc ggcagcaaac gcaaagcgcc acgctggaag aagcatttat
     4681 aaatctgtta ccgcaagcac aacgccaggc gcatcaggcg gtagtgatcc caccgtatca
     4741 acctgaaaac gcagagattg ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt
     4801 cgttgccgtt gatcacgtca atttccgcat tccacgcggg gagatttttg gattccttgg
     4861 ttcgaacggc tgcggtaaat ccaccaccat gaaaatgctt accggactgc tgcccgccag
     4921 cgaaggtgag gcgtggctgt tcgggcaacc ggttgatcca aaagatatcg atacccgccg
     4981 tcgggtaggc tatatgtcgc aggcgttttc gctctataac gaactcaccg tgcggcaaaa
     5041 ccttgagtta catgcccgtt tgtttcacat cccggaagcg gaaattcccg cgcgagtggc
     5101 tgaaatgagc gagcgtttta agctcaacga cgttgaagat gttctgccgg agtcattgcc
     5161 gctcggcatt cgccagcggc tttcgctggc ggtggcggtg attcatcgcc cggagatgtt
     5221 aatcctcgat gaacctactt ctggcgtcga tccggtggcg agggatatgt tctggcagtt
     5281 gatggtcgat ctctcgcgcc aggacaaagt gactatcttc atctccaccc actttatgaa
     5341 cgaagcggaa cgttgcgacc gcatctcact gatgcacgcc ggaaaagtgc ttgccagcgg
     5401 tacaccgcag gaactggttg agaaacgcgg agccgccagt ctggaagagg catttatcgc
     5461 ctatttgcag gaagcggcag ggcagagcaa cgaagccgaa gcgccgcccg tggtacacga
     5521 catcacccac gcgccgcgtc agggatttag cctgcgccgt ctgtttagct acagccgccg
     5581 cgaagcactg gaactgcgcc gcgatccggt ccgttcgacg ctggcgctga tgggaacggt
     5641 gatcctgatg ctgataatgg gttacggcat cagtatggat gtggaaaacc tgcgctttgc
     5701 ggtgctcgac cgcgaccaga ccgtcagtag ccaggcgtgg acgctcaatc tctccggttc
     5761 ccgttacttt atcgaacagc cgccgctcac cagttatgac gagcttgatc gtcggatgcg
     5821 tgcgggcgat atcacggtgg caattgagat ccctcccaat ttcgggcgcg atatcgcgcg
     5881 tggtacgcct gtggaactcg gcgtctggat tgacggagcg atgcccagtc gtgccgaaac
     5941 ggtaaaaggt tacgtgcagg caatgcacca gagctggtta caggatgtgg cgagccgaca
     6001 atccacaccc gccagccaaa gcgggctgat gaacattgag acgcgttatc gctataaccc
     6061 ggacgtaaaa agcctgccag cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat
     6121 cccgtcaatg ctaagcgccc ttagcgtggt gcgggaaaaa gagcttgggt cgattatcaa
     6181 cctttacgtg acccccacca cgcgtagtga atttttgctt ggtaaacagt tgccatacat
     6241 cgcgctgggg atgctgaact ttttcctgct ctgcggcctg tcggtgtttg tgtttggcgt
     6301 accgcataaa ggcagtttcc tgacgctcac cctggcggcg ctgctgtata tcatcattgc
     6361 caccggaatg gggctgctga tctccacctt tatgaaaagc cagattgccg ccattttcgg
     6421 aacggcgatt atcacgttga tcccggcgac acagttttcc gggatgatcg atccggtagc
     6481 ttcgctggaa gggcctggac gttggatcgg cgaggtttac ccgaccagtc attttctgac
     6541 tatcgcccgc gggacgttct cgaaagcgct ggatctgact gatttgtggc aactttttat
     6601 cccgttgctg atagccatcc cgctggtgat gggcttaagt atcctgctgc tgaaaaaaca
     6661 ggagggatga tgcgccattt acgcaatatt tttaatctgg gtatcaaaga gttgcgcagt
     6721 ctgctcggtg ataaagcgat gctgacgctg attgtcttct cgtttacggt gtcggtgtat
     6781 tcgtcagcga ccgttacgcc aggatcgttg aacctcgcgc cgatcgccat tgccgatatg
     6841 gatcaatcgc agttatcgaa ccggatcgtt aacagcttct atcgtccgtg gtttttgcca
     6901 ccggagatga tcaccgccga tgagatggat gccggactgg acgccggacg ctataccttc
     6961 gcgataaata ttccgcctaa ttttcagcgt gatgtcctcg ccggacgcca gccagatatt
     7021 caggtgaacg tcgatgccac gcgcatgagc caggcattta ccggcaatgg gtatatccag
     7081 aatattatca acggtgaagt gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg
     7141 ttggtatcgc tggaaacccg gatgcgcttt aacccgaacc ttgatcccgc gtggtttggc
     7201 ggggtgatgg caatcatcaa caacattacc atgctggcga ttgtattgac cggatcggcg
     7261 ctgatccgcg agcgtgaaca cggcacggtg gaacacttgt tggtgatgcc gataacgccg
     7321 tttgagatca tgatggcaaa ggtctggtcg atggggctgg tggtgctggt ggtgtcggga
     7381 ttatcactgg tgctgatggt gaaaggcgtg ctgggtgtac cgattgaagg ctcgatcccg
     7441 ctgtttatgc tgggcgtggc gctcagtctg tttgccacca cgtcaatcgg catttttatg
     7501 gggacgatag cgcgttcaat gccgcaactg gggctgctgg tgattctggt gctgctgccg
     7561 ctgcaaatgc tttccggtgg atccacgccg cgcgaaagta tgccgcagat ggtgcaggac
     7621 attatgctga ccatgccgac gacacacttt gttagcctcg cgcaggccat cctctaccgg
     7681 ggtgccggat tcgaaatcgt ctggccgcag ttcctgacgc tgatggcaat tggcggcgcg
     7741 tttttcacca ttgcactgct gcgattcagg aagacgattg ggacaatggc gtaaatacaa
     7801 ttgcccgatg cgtaagctta tagggactac gcatcggtgc aatttattga attttagttg
     7861 tttttaggcc ggataaggcg tttatgaact gacccccaaa acctggacag tttcagttaa
     7921 ccggcttttt gatactcagt cctgtattca gggcaagatt acgaaagccc
//
LOCUS       DAZFJF010000178         7969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFJF010000178 DAZFJF010000000
VERSION     DAZFJF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.179"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            157..681
                     /locus_tag="ACHKAU_004498"
     CDS             157..681
                     /locus_tag="ACHKAU_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO5533429.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            765..1451
                     /gene="lpfB"
                     /locus_tag="ACHKAU_004499"
     CDS             765..1451
                     /gene="lpfB"
                     /locus_tag="ACHKAU_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO5533430.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1475..4006
                     /gene="lpfC"
                     /locus_tag="ACHKAU_004500"
     CDS             1475..4006
                     /gene="lpfC"
                     /locus_tag="ACHKAU_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO5533431.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            4017..5072
                     /gene="lpfD"
                     /locus_tag="ACHKAU_004501"
     CDS             4017..5072
                     /gene="lpfD"
                     /locus_tag="ACHKAU_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO5533432.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            5078..5602
                     /gene="lpfE"
                     /locus_tag="ACHKAU_004502"
     CDS             5078..5602
                     /gene="lpfE"
                     /locus_tag="ACHKAU_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO5533433.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5855..7546
                     /gene="eptB"
                     /locus_tag="ACHKAU_004503"
     CDS             5855..7546
                     /gene="eptB"
                     /locus_tag="ACHKAU_004503"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO5533434.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7638..7714
                     /locus_tag="ACHKAU_004504"
     tRNA            7638..7714
                     /locus_tag="ACHKAU_004504"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7672..7674,aa:Pro,seq:cgg)
     gene            7749..7882
                     /locus_tag="ACHKAU_004505"
     ncRNA           7749..7882
                     /ncRNA_class="other"
                     /locus_tag="ACHKAU_004505"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2082 a         1825 c         2005 g         2057 t
ORIGIN      
        1 tttgtgtgca attaattgaa gttgaagtac atttcaattt attgcaaaaa ctaatcttca
       61 gtaaaaataa ttttcttttt tagcaccatt tgtataatct gcgccccgtt gtatgaacaa
      121 catcaagcat atttatctat ataaatggag ttataaatga aaaaggttct gtttgcctta
      181 tctgcgcttg cgctgacctc tacttccgta ttcgcagctg atgcgggcga cggtactgtt
      241 aaattcaccg gtgagattgt tgacgccgct tgtgttgtgt ctccagacac tcaaaaacaa
      301 gaagttgttc tgggtcaggt gaacaagtct gtatttactg ctatgggtga taaatccaca
      361 gcaacgccgt tcaaaatcaa actggaaaac tgcgatatct ccactttcaa aaatgtggaa
      421 atcagcttta acggcgttgg cgatgcagac aacagcaaac tggtttctgt aagcactgaa
      481 ccaggtgccg caactggcgt aggtatcggt atttatgata acaccaatac cctggttgac
      541 ctgaatactg gtaaatctgc tactgtcctg aaagaaggtc agaccgttct gtatttcacc
      601 gctaactatg tctctaccaa agatgcagta accacgggtt acggtaacgc tgaagtcgac
      661 ttcaacctga cttacaacta atcgatcctg acggaacgat catctctcaa tggcaacaga
      721 gactgtgttg ccattattca caccaatgac tcagggaccg aatcatgaaa cgtattagct
      781 ttgctgtgct ggcactttta atgaccgcac aacagtgttt tgctggtggc gtagccttaa
      841 gcacgacgcg tgttatttat gatggcggca agaaagaagc gtcgctgacc gttgaaaacc
      901 ataataaaaa tgaagagttt ttgatccagt catggattga tgatgtgaat ggcaataaaa
      961 aaacaccgtt tattattaca ccgccattat tcaaacttga tcctgataaa aataacgtct
     1021 tacgtatcgt caatattgaa gataccttac ctaaagatcg tgaatccgtt tactggatta
     1081 atgtaaaggc cattcctgcg aaaagtgctg aaaatgaaaa taaaaacgtc ttacagatag
     1141 ccgttcgcac ccgtttgaag ttgttctatc gtccaaaagg tttgccggga agcaacctcg
     1201 aggccatgaa acagttacgt ttccagcgtc aaaataacca aattgtggtt aataacgact
     1261 ccgcttataa cctgaccttt aatcagtttt ccattaatgg acaaaagatt gaacgggcgg
     1321 gaatggttct cgcgaaagga aagttaaaca ttaaccttcc agccggtaca ggcaacgcgc
     1381 acgaagtgaa atacagcatt attaatgatt tcggtgttgc tggcgaaatg ctgacgaagc
     1441 cgattaactg atccttttac aaggcgcttt tactatgcat tggatgcatt tacctctcta
     1501 tcactacaga gcgcattttt ccttctctct gctggcatta acgattgcca gctcgttacc
     1561 tgcgtatggc ggaaaattta accctaaatt tctcgaaaac gttcagggta tcgatcaaca
     1621 catcgacttg tcggtatacg actcacctgt cggccagcaa atccccggta aataccgggt
     1681 ttcggtgttt gtaaacgaag aaaaaatggc ttcccggacg ctggatttta gtaccgccag
     1741 cgaagcgaaa cgcaaagcca gcggtgagtc gcttatgcct tgccttagcc gggtgcaact
     1801 ggaagaaatg ggcgtgcgcg ttgacagctt tccggcgctg aagatgtcac caccagaagc
     1861 ctgcgtagcg tttgatgaaa tcattcccca ggccaccagc cgctttgatt ttaatactca
     1921 gacgctgcat ctttcctttc cacaggcggc aatgatgatg accgctcgcg gcacggttga
     1981 tccttctcgc tgggacgaag gaatacctgc gctgttgctg gattacagct ttagtggcag
     2041 caatggacgc aatgaaggta caggttcatc ttcagattcg accagtgaca gttattacct
     2101 gaacctgcgc agcggtttga acgttggtcc gtggcgttta cgtaataaca gtatctggaa
     2161 ccgtaccgat ggcaaaaacc aatgggataa cgttggtaca tcgttaaacc gggcaattat
     2221 tccacttaaa tcacagataa ctctgggtga taccgccacg cctggcgaga tttttgatag
     2281 cgtacaaatg cgtggggcgt tactggcatc cgacgatgaa atgttgccgg acagccagcg
     2341 tggctttgct ccggtagtgc gcggcatcgc caaaagcaac gctgaagtga gtatcgaaca
     2401 gaacggctat gtcatttatc gcacttttgt tcagccgggt gcatttgaga ttaacgatct
     2461 ctacgcaacc tccggcagcg gcgacctgac ggtgatcatt aaagaagccg acggaagtga
     2521 acaacgtttt atccagccgt tttcggcggt ggcgattttc cagcgtgaag ggtatctgaa
     2581 atacagtctg gctgccggtg aatatcgtgc tggtaattac gacagcgata aaccacattt
     2641 cgggcagttt accgccatgt acggcttgcc gtggggtatg accgcctacg gtggtgcatt
     2701 gttgtcggca gattacaacg ctttagctct gggcctgggg aaaaacttcg gtaccattgg
     2761 cgcagtatcg gtggatgtca ctcaggcgaa aagtcagtta cgcaacaatg aaaaagagga
     2821 aggccagtct tatcgcttcc tctattcgaa aagttttgag ggtggcactg acctgcgtct
     2881 gttgggctac aaatactcaa ccagcggcta ctacaccttc caggaagcaa ctgatgttcg
     2941 cagtgacgcc gacagtgatt accgccgtta tcacaagcgc agccagatac agggcaatat
     3001 tacccagcaa ctgggagact acggctcggt ttatttcaat atgacgcagc aggattactg
     3061 gaacgttgac ggtaaggaaa actccttaag cgccggttat cacggtcaca ttggtcgggt
     3121 gaattacagc gtggcctata cctggacgag aagtccggaa tgggaagaag acgatcgctt
     3181 gtggtcattc agtgtttcca tccctctggg gggcgcgtgg agcagttatc gcatgactac
     3241 cgaccagaat ggcaagacgt cgcaacaggc ctctgttagc ggcacgttac tggaagatcg
     3301 taacctgagc tataacgttc agcagggtta caccagtaat ggcgtaggct acagtggcag
     3361 cgtcaacatg gggtatatgg gtggctctgg taacattgac gtaggctata actattctaa
     3421 agataaccaa caggttaact atggcgtgcg cggcggtgtc atcgttcaca gtgaaggtat
     3481 aacgctttcg cagcctttag gcgaaagtct ggcgattgtt tctgcaccgg gcgcgcgtgg
     3541 tggacatgtg gttaacagca gcggcgtaga agttgactgg atgggcaacg ccgtggtgcc
     3601 ttatctcaca ccatatcgtg aaaccattgt ggaattgcgt agcgacacgt tggggcaaaa
     3661 cgttgaacta caggaagcgt tccagaaagt cgtgcctact cgtggtgcag ttgtgcgttc
     3721 ccgctttgat acccgcgtcg gttaccgtgt gctgatgagc ttaaaacagg cgaatggcaa
     3781 cgcagtaccg tttggtgcaa ccgcagcgtt aattgatgag agcaaacctg ccagcagcat
     3841 tgtgggtgaa gaaggacagc tttatatcag cgggatgcca gaagagggcg aattgcaggt
     3901 gagctggggg aacgagcagg cgcaacgttg ccgtgtgcca ttccgcttgc cagagaacaa
     3961 ggacaatgcg gctatcgtga tggttaatgc ggtatgcgag aaataaggat atttagatga
     4021 aaattaaaaa tttgttggca ggtatggtgc tgttagggac gagcactttt gctggaaata
     4081 tatgggctgc ggattgggga ccatgtcaga catcaaatgg ggtggcccat gagtatagtt
     4141 ttgatttcgt acagactatc caggtacctt cagaaaataa ggctgggaaa atattaactc
     4201 agccctttgc tttaggtaca aaatattccg cttattgtga atgcccggat cccattccag
     4261 ataatggagt tgtgacttat tttaaaggtg taacgcttct gcctgagcct ggaactgtag
     4321 atgggtacta taaattcaat aattacattg atattctcac caaaatatat gtctacacac
     4381 aattagatat cccggtgcct tttaccgatc gaagcaatgg cacggcacag aaagaatgta
     4441 cgccctatac tgctaacaat tgggggacag gtggtaaagg aagtatcagt atttatattt
     4501 cacatccttt tgttgggcaa atgattattc ctaaaacacg agttgcgtcc ctctttggga
     4561 caaaaaagaa aggtgtttac aatgatagtc agcccatggc caatgtcagt atttccggga
     4621 gtattacagt tactcaaggc tgtgaacttg ccgctggcac cgtactggat attccattcg
     4681 gagaatatca ggcccatgat tttaaagggc gtgccggaca accaccacaa aatgttcaga
     4741 aggtacaaaa agaattaagc tttgactgca ataatatttc agatggcgtc aaaatttatc
     4801 tcagtattga tgcaacacca aatactgcct atcccagcgc aattgattta ggcaacgccg
     4861 atgttggtgc agtcattgag gacggtaaag gtaatattct taagccgaat gacagtaata
     4921 gcttattaga aatgaaccca ggctcgctgt atgaagatgt caaacgcaaa gcgacaacca
     4981 caattaccgc ttatcccgtt agcactaccg gaaaattacc cgctgccggg gattatagcg
     5041 gtattgctac catacatgtc gaactggagt aacagtcatg aagcgaacaa tacttgcgtt
     5101 atgcctgctg cctctttcct tttcgtcatt cgcggcaacc acggatttag ggccgaaagg
     5161 aacattgaaa ttcaccctta aaatcagcca gggtgcttgc gaactggtga aagacagcgt
     5221 tgaggttgac atgggcaccg cggttctcaa aaagccggta agaatcggaa cggaaattaa
     5281 tcccactcct ttttccatcg gcctgaaaaa ctgtagcgaa gtggtcagag cctacgtgac
     5341 tatggacggt acacccaatg cagacgactc aaatcttttt gcgctggatg cgggtggcgc
     5401 tacaggcgtt gggctgaaaa tcaaaaccgc tgctggagta caacagttcc cggaaaatac
     5461 aacgccgaca ccggttgagt ttgcaattaa cggtgatggc gcacatcaac tgaactatat
     5521 cgccagctac gtgcctattc gcgccgatgc aactattggt cgcgcagatg caacggtcga
     5581 ctttactgta cagtacgaat aatttcacat cttaagggcc agttaactgg ccctttttac
     5641 tgctttttgt aacgctaaag tctcttttca aacttgcatt tttgtaaatt tgtgcttcat
     5701 gcacactctt tccccacact ttttcgcttt gctgtggtct acttattcgc gcgtgtagat
     5761 tttacttatc tgactacctc cgcacttttc ccctgccggg cctgaaaagc cgctaagcag
     5821 ggtgttatca cctgtttgtc cagggtttgt ttgcatgaga tacatcaaat cgattacaca
     5881 gcagaagctg agctttttgc ttgcaatcta tattggcctt tttatgaatg gcgcggtttt
     5941 ttatcgccgc ttcggcagct atgcgcacga ttttaccgtc tggaaaggcg tttctgctgt
     6001 tgttgaactg gccgccaccg tactggtgac cttcttttta ctacgtcttc tatcgctgtt
     6061 tggccgccgc agctggcgta ttctggcgtc gctggtggtg ctcttttccg caggtgccag
     6121 ctattacatg accttcctta atgtggtcat tggttatggc atcatcgctt ccgtcatgac
     6181 caccgatatc gacctgtcaa aagaagttgt tggtctgaac tttattctct ggttaatcgc
     6241 cgttagtgca ttgcctctta tccttatctg gaataaccgc tgtcgctaca ccttgctccg
     6301 acaactgcga accccagggc agcgtattcg cagcctggcg gtcgtcgtac tggcgggtat
     6361 tatggtttgg gcaccgattc gtttgctgga tatccagcag aagaaagtgg agagggcgac
     6421 cggcgttgat ttgccgagtt atggcggtgt cgtagcgaac tcttatctgc catcaaactg
     6481 gctttctgcg ttggggctgt atgcctgggc gcgggtcgat gaatcttccg ataataattc
     6541 attgcttaat ccggcgaaga aattcactta tcaggcaccg caaaacgttg atgacactta
     6601 tgtcgtgttt atcatcggtg aaaccacgcg ttgggaccat atgggcattt tcggctatga
     6661 gcgtaatacc acgccgaaac tggcccagga gaaaaatctg gcggcgttcc gtggttactc
     6721 ctgtgatacc gcaaccaaac tctcactgcg ttgcatgttt gtacgtcagg ggggcgcgga
     6781 agataatccg cagcgcacat taaaagaaca gaacattttc gcggttctga agcagttagg
     6841 attcagttct gacctctacg ctatgcagag cgaaatgtgg ttctacagca acacgatggc
     6901 ggacaacatt gcttatcgtg agcagattgg tgcggagcca cgtaatcgtg gcaagccggt
     6961 agatgatatg ttgctggtag acgaaatgca gcaatcgcta gggcgcaacc cggatggtaa
     7021 gcatctgatc attctgcata ccaaaggttc gcattttaac tacacccagc gttatccgcg
     7081 tagcttcgcg cagtggaagc cggaatgtat tggtgttgat agcggctgta ccaaagcgca
     7141 gatgatcaac tcctatgaca actcggtgac ctatgtggat cactttatct ccagcgtgat
     7201 tgatcaggtt cgcgataaga aagcgattgt gttctacgca gctgaccacg gtgagtcaat
     7261 taatgaacgc gagcacctgc acggcacgcc gcgtgaactg gcaccgccgg agcagttccg
     7321 cgtaccgatg atggtctgga tgtcagataa atatctggaa aatccggcca atgctcaggc
     7381 atttgcgcag ctgaaaaaag aagccgacat gaaagtgcca cgccgtcatg tagagctgta
     7441 cgacaccatc atgggttgtc ttggctatac ttcaccggat ggtggaatta acgaaaacaa
     7501 caactggtgt cacatcccgc agacaaaagt ggcagcggct aactaagcgg cctgctgact
     7561 ttctcgccga tcaaaaggca ttttgctatt aagggattga cgagggcgta tctgcgcagt
     7621 aagatgcgcc ccgcattcgg tgattggcgc agcctggtag cgcacttcgt tcgggacgaa
     7681 ggggtcggag gttcgaatcc tctatcaccg accaaattcg aaaagcctgc tcagcgagca
     7741 ggcttttttg catttgaagc ccatgaggat gagaacctcc gggggcgggg aggttcgact
     7801 cgagcgaagc gagagaacgt tgcgccagca acggcccgca gggcgagcca cgaagtggcg
     7861 agtaatcctc tatcaccgac caaattcgaa aagcctgctc aacgagcagg cttttttgca
     7921 tttgtagctc atgaggatga gaacctccgg gggcggggag gttcgactc
//
LOCUS       DAZFJF010000179         7943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFJF010000179 DAZFJF010000000
VERSION     DAZFJF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.180"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(418..1095)
                     /gene="bioD"
                     /locus_tag="ACHKAU_004506"
     CDS             complement(418..1095)
                     /gene="bioD"
                     /locus_tag="ACHKAU_004506"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5533435.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(1088..1843)
                     /gene="bioC"
                     /locus_tag="ACHKAU_004507"
     CDS             complement(1088..1843)
                     /gene="bioC"
                     /locus_tag="ACHKAU_004507"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO5533436.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(1830..2984)
                     /gene="bioF"
                     /locus_tag="ACHKAU_004508"
     CDS             complement(1830..2984)
                     /gene="bioF"
                     /locus_tag="ACHKAU_004508"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO5533437.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(2981..4021)
                     /gene="bioB"
                     /locus_tag="ACHKAU_004509"
     CDS             complement(2981..4021)
                     /gene="bioB"
                     /locus_tag="ACHKAU_004509"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO5533438.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4108..5397
                     /gene="bioA"
                     /locus_tag="ACHKAU_004510"
     CDS             4108..5397
                     /gene="bioA"
                     /locus_tag="ACHKAU_004510"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO5533439.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            5456..5932
                     /gene="ybhB"
                     /locus_tag="ACHKAU_004511"
     CDS             5456..5932
                     /gene="ybhB"
                     /locus_tag="ACHKAU_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO5533440.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            6436..7089
                     /locus_tag="ACHKAU_004512"
     CDS             6436..7089
                     /locus_tag="ACHKAU_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFO5533441.1"
                     /translation="MPIIKNCLSSISNILRNEKTSYSLIKTEQTGKLLNRKITTTPTP
                     AKLLSYRNADLIKENYITEKVLSIFNIKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAK
                     VIDWYNPQKNAFGIMMGTPRRSADIAKEESRNALNFMIMEKDTFNEKILNSNANLQKK
                     YGTTENSSWVSASVGSLLDKGAKVYPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(7102..7323)
                     /locus_tag="ACHKAU_004513"
     CDS             complement(7102..7323)
                     /locus_tag="ACHKAU_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533442.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(7407..7787)
                     /locus_tag="ACHKAU_004514"
     CDS             complement(7407..7787)
                     /locus_tag="ACHKAU_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533443.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
BASE COUNT         2002 a         2083 c         1892 g         1966 t
ORIGIN      
        1 cgagataaaa ctccggcctt accgggttat ccccaaagca acggcttttt taacatttgt
       61 caagatgagt gatgacagtt ttatggcaag agatgcctgt tcagtgactg ccattgcatt
      121 tatctaacca gttaaaaaat aaaagatatt tctttgagcc gtctttaacg ccaattcgcg
      181 tgcaagccgc gtattctctc gcttgcctcg tgttttctaa ctctaataca catggttatc
      241 cacaggaata gtggataact gtctccagcc cctatccccc gccgcctggg caattcccac
      301 attcgccgca tgaggcggaa gtaaaatttt tttcgtgatt attttagaat ttaattggtt
      361 aaattgcagt caatcgaaga cgcgatctcg ctcgcaattt aaccaaatac aggatagcta
      421 caacaaggca aggtttatgt actttccggt tgccgcattt tctggatttt ctgcaagcca
      481 ggggatctct cccagcagcg gcgcgggaat catgcgggtg agcgtggtca tatattcagc
      541 gtgacgtttt cccggaggcg taacatcgtt cgccacccaa cccgccagag tcagtccggc
      601 gtgttgtatt gcctgcgcag tcaacatcgc gtgattaata cagccgagtt tcacaccaac
      661 taccagtatc accggcagtt gttcctgtgt tacccaatct gcaaaagtga aagtgtcaga
      721 aagcggcgta aaccagccgc cagcaccttc cactaacacc cagtcagcct gttgttcaag
      781 cgcgcgtaat ccggcgctca ttaccaatga ttctatcggc ctgccctctt gcgcgctgat
      841 gatgtgcggc gaggtgggtt ctgcgaaggt gtaaggattt accgttgcgt aatccagctg
      901 caggctgctg ttgtgctgta acgccagcgc gtcactatta cgtaagcctt ccggggtctt
      961 ttcgctgcca gaggcgaccg gtttataacc tgccgtccga taacctactg ccttcgcggc
     1021 ttgtaaaagt gcacaactgg cgacggtttt ccccacttcg gtatccgttc cggtgacaaa
     1081 ataacgttta ctcacgagca atcactccca aaaaaagatg atacgtcaga ggatatcgcc
     1141 cctgttgttg cggccaggcc agttgcaatc gctgcaattg cgaacgcgtt aatattcgcg
     1201 ggtcgcgccc ttcatgaaga tgcgtagcac cgatgccttt cagcgaacgc atggcactga
     1261 gcgcatcatc aaaccatagt gtgatgggct gaatatgatg ttgataatgc acgccgttca
     1321 gcgactgttc gatttcatct ggcggtaaaa agcgattagc atgcggacgc tcgtccaccg
     1381 cctgccacgc ctgatgcagt tcgggtaacg atccctgcac cagcgtggta aacgccacca
     1441 cgcctccggg gcgcaccacc cgatacagct cgcggagtgc cgtggataaa ttaccgcacc
     1501 actgcactgc gagattgctc catgcaagat cgaacgtcgc agtcgctaac ggcagggatt
     1561 cgatatctcc cgccagataa tggtctgcgg catccttctg gcgtgcctga acaagcattg
     1621 gcggcgagag atctaaggcc gtcacctgcg cgtgacgttc ccgccagtgg cggctcatcc
     1681 agccaggtcc acaacccgcg tccagtacgt gggtgtattt acgctgtggg agcattgcca
     1741 gtaagacgtc agcactctgg cgctgtagat ctgcgtgttg ctcatagagt gcggctgccc
     1801 gaccaaatgc cgctgcaatg gcttgtttat taaccgttgc catgcagcac ctccagcaga
     1861 cggtcgatat cctgcatttc atgcgctgcg gttagcgtta agcgcagtcg cgcagtacca
     1921 gcgggtacgg ttggcgggcg aatcgccgtg acccagcagc cttgctggcg cagtttttct
     1981 gccagttgta acgcacggct gttatcaccg acgatcaatg gctggatggc gctgcatgaa
     2041 tcagccagcg taaacggcaa atcctgtact ccggcacgaa aacgcgcaat gagtgacacc
     2101 agtttttcgc gccgtgcatc cccctcatca cggcgaatga ccgccagtga cgcacgtaat
     2161 gcctgcgcct gagcgggcgg catactggtg ctgtagataa ggtggcgggc gaattgcagc
     2221 agataatccg ccaccgtact ggagcaaagc accgcagccc cactgacgcc aaatcctttg
     2281 ccaaaagtca ccaccagcaa ttctggtttt accttttgca gccagcagct gccgcgcccc
     2341 tgctccccga taacgcccgt gccgtgggca tcatcgacca tcaaccagcc attgtgctgt
     2401 tgcgttacct gctggatttc cgccagtggc gcaatatcgc cgtccatgct gaacacgcct
     2461 tctgtcacca ccagttgctg ccccggacag ggggaagcaa gcagtcgcgc cagatgagtg
     2521 acatcgttat gaacaaaacg gcgaagctgc gacgggctta aactggcagc ctccagcaat
     2581 gaggcatggc taagccggtc agcgacaatg cggtcctctt ttgccatcat cgcggcaatg
     2641 accgcctgat tagcggcaaa accagagata aatagcagtg cccgcgaata gcccagccac
     2701 tcggccagtt cttcttccag cgcctgatgc gcaacgctat aaccgctgac gtgaccggaa
     2761 ccgccgctac cgacgccaaa ttgctccgca ccctgctgcc aggcacggat aatttgcgga
     2821 tgatggctta aacctaaata atcgttactg gaaaagttca gatactggcg atcatccgcc
     2881 accagccagc gtccggctcc ttgcgccact ggataacggc gacgcagggc atcggcagca
     2941 cgccgcgcat cgagcgccgc gttgattttc tcctgccagc tcataatgct gccgcgttgt
     3001 aatattcgtc ggtgtccggg gtcatcagcg cctgttcaag acgttgctgt tgttcgttat
     3061 cccctgccag cacggcagtt tgctgcggat ttagccccag tttgcggaac agttgcaggt
     3121 ctttatcttc ttccggattc ggcgtggtca gcagtttgca accgtagaaa atcgagtttg
     3181 cgcctgccat aaagcacatc gcctgagtct gttcgttcat ctgctcgcgt ccggcagaaa
     3241 ggcgcacgta agaggtcggc atcatgatcc gcgcgaccgc aatggtgcga ataaaatcaa
     3301 aggcatcgac atcatcgtta tccgccagcg gcgtgccttt caccttcacc agcatgttga
     3361 ttggtacgct ttccggcggc gtcggcaggt ttgccagttg cagcaataat ccggcgcgat
     3421 cttttaccgt ttcgcctaag cccacaatgc cgccagaaca gactttgatc ccggcatcgc
     3481 gcactttttc cagcgtatcg aggcgttcct gataagtgcg ggtggtgatg atattgccgt
     3541 aaaactccgg cgaggtgtcg aggttgtggt tgtagtaatc cagcccggca ttcgcaaggc
     3601 gctgcgcctg agattcactc aacgtgccca gcgtcataca cgcctccagc cccatcgctt
     3661 ttaccccctg caccatttgt tccaggtacg gcatatcgcg ttcgtgggga ttcttccacg
     3721 ccgcgcccat acagaaacgc gtcgatcccg cagctttcgc tttgcgcgcc gactccagca
     3781 cctgttcaac ttccatcaac cgctcggctt ccagcccggt tttgtagcgc gagctttgcg
     3841 ggcagtattt gcaatcttcc ggacaagctc cggtcttaat cgacagcaaa gtgctgacct
     3901 gcacctgacg aggatcgaaa tgctgtcgat gcacctgctg cgcttcaaac agcagatcca
     3961 gcaacggttt ttcaaataat tctgtgactt gcgacaatgt ccagcgtggg cggtgagcca
     4021 tggggcttct ccaaaacgtg ttttttgttg ttaattcggt gtagaattgt aaacctaaat
     4081 cttttcaatt tggtttacaa gtcgattatg acaacggacg atcttgcctt tgaccaacgc
     4141 catatctggc acccatacac atccatgacc tcccctctgc cggtttatcc ggtcgcgagc
     4201 gccgaaggtt gcgagctaat tttgtctgac ggcagacgcc tggttgacgg tatgtcgtcc
     4261 tggtgggcgg cgatccacgg ctataatcac ccgcagctta atgcggcgat gaagtcgcaa
     4321 attgatgcta tgtcgcatgt gatgtttggc ggtatcaccc atgcgcccgc catcgagcta
     4381 tgccgcaaac tggtggcaat gacgccgcaa ccgctggaat gcgtttttct cgcggactcc
     4441 ggctccgtag cggtggaagt ggcgatgaaa atggcgttgc agtactggca ggccaaaggc
     4501 gaagcgcgcc agcgttttct gaccttccgc aatggttatc atggcgatac ctttggcgcg
     4561 atgtcggtgt gcgatccgga caactcaatg cacagcctgt ggaaaggcta tctgccagaa
     4621 aacttgtttg ctcccgcccc gcaaagccgc atggatggtg aatgggatga acgtgatatg
     4681 gttggctttg cccgcctgat ggcggcgcat cgtcatgaaa tcgcggcggt gatcattgaa
     4741 ccgattgtcc agggcgcagg cgggatgcgc atgtaccatc cggaatggtt aaaacgaatc
     4801 cgcaaaatgt gcgatcgcga aggtatcttg ctgattgccg acgagatcgc caccggattt
     4861 ggtcgtaccg gcaaactgtt tgcctgtgaa catgcagaaa tcgcgccgga cattttgtgc
     4921 ctcggtaaag ccttaaccgg cggcacaatg accctttccg ccacgctcac cacgcgcgag
     4981 gttgcggaaa ccatcagtaa cggcgaagcc ggttgcttta tgcatgggcc aacttttatg
     5041 ggcaatccgc tggcctgcgc ggcagcaaac gccagcctgg cgattctcga atctggcgac
     5101 tggcagcaac aggtggcggc tattgaagta cagctgcgcg agcaacttgc ccccgcccgt
     5161 gatgccgaaa tggttgccga tgtgcgcgtg ctgggggcaa tcggcgtagt cgaaaccact
     5221 catccggtga atatggcggc gctgcaaaaa ttctttgtcg aacagggtgt ctggatccgg
     5281 ccttttggca aactgattta cctgatgccg ccctatatta ttctcccgca acagttgcag
     5341 cgtctgaccg cagcggttaa ccgcgcggta caggatgaaa catttttttg ccaataacga
     5401 gaagtccgcg tgagggtttc tggctacact ttctgcaaac aagaaaggag ggttcatgaa
     5461 actcatcagt aacgatctgc gcgatggcga taaattgccg catcgtcatg tctttaacgg
     5521 catgggttac gatggcgata atatttcacc gcatctggcg tgggatgatg ttcctgcggg
     5581 aacgaaaagt tttgttgtca cctgctacga cccggatgcg ccaaccggct ccggctggtg
     5641 gcactgggta gttgttaact tacccgctga tacccgcgta ttaccgcaag ggtttggctc
     5701 tggtctggta gcaatgccag acggcgtttt gcagacgcgt accgactttg gtaaaaccgg
     5761 gtacgatggc gcagcaccgc cgaaaggcga aactcatcgc tacattttta ccgttcacgc
     5821 gctggatata gaacgtattg atgtcgatga aggtgccagc ggcgcgatgg tcgggtttaa
     5881 cgttcatttc cactctctgg caagcgcctc gattactgcg atgtttagtt aatcactctg
     5941 ccagatggcg caatgccatc tggtatcact taaaggtatt aaaaacaact ttttgtcttt
     6001 ttaccttccc gtttcgctca agttagtata aaaaagctga acgcgaaacg ctaaagacca
     6061 ttaatatcaa tgtgttacaa tatatttagt ctaaaaaata gactgcataa tactacaaaa
     6121 cacaacatat ctagtaacta tgaatcaact acttagatag tattagtgac ctgagacaga
     6181 gcattagcgc aaggtgattt tatgtcttct tgcgctaatt ttttgtcata aaaacatgtg
     6241 gcagactttg tatatctctg ctttgccacg ccacaccatc atgcattatg gctatgccta
     6301 aacagaatca ataagaatag aacataaaaa atgacaactc tactagacaa ttaactacaa
     6361 aaatgatagc aattagttta tataatataa gacttcctat caaaagttca tccatatgaa
     6421 gacaaggaat ttattatgcc aatcataaag aactgcttat catcaattag taacatatta
     6481 cgcaacgaaa aaacatcata ttctctaata aaaacagagc aaacaggaaa actgttaaac
     6541 agaaaaataa ccaccactcc cacaccagcg aaactattat catatcgaaa tgcagattta
     6601 ataaaagaaa actacattac agaaaaagtt ttatctattt tcaatataaa acgggatttc
     6661 gttgctgtaa gaatccaaag taatcagttt actgatttaa aaaacaagac aattcagggt
     6721 cataaagaca ctgtcgcaaa agtcatagac tggtataacc cacaaaaaaa cgcatttggt
     6781 atcatgatgg gcacaccaag acgttctgct gatatagcaa aggaagaatc cagaaacgct
     6841 ctaaatttca tgataatgga aaaggataca tttaatgaaa agatacttaa cagcaatgct
     6901 aatttacaaa aaaaatacgg cacaacagaa aatagtagtt gggtatcggc ttctgttggt
     6961 tctcttctgg acaaaggagc caaagtatat ccagatatat catgctctct gagactggga
     7021 gaaccattca ttattacact tccagaaaca gtcaggctgg atgttaatat ccacccactc
     7081 aaaaaatgat cctgaacgtt tttatgcagg caaatgcgtc cacatcaagg cttcaacctt
     7141 gtgctctccc cctgtcacag gataaatttg cctgtaaaaa tcaaactctc cctgctcatt
     7201 accaatacca atcagttgaa tgcagttgcc atctacaata aatttttccg ctgacgtaac
     7261 caaaataaag gcttccggat gttcattagg aaaggtgtta gcaagagtac actgaatatc
     7321 catctccaac aaaatctcct gattattagc gtaaactcat cgacgaaatc gttactatcc
     7381 cctgggcaca tatgtaccat tgaatactac tgttctccag attctgataa tccatggcga
     7441 acaataagaa cactacagcc gctttgctga ttagttagcc ataacggtga actttgctgg
     7501 atagtttgag aaatatcatt tcgccggagg cttatacact gattactcaa gcttacaact
     7561 gcaggtgcat caaagcgctc cggactatca gtatcacgca tagctgtaaa cagaatataa
     7621 tcactaccag ccatatcata ttccgtttct tttagtctta acaagccatc ctctcttgta
     7681 atattaactg taattaaaga tgtctcatca tgatgggcat agcgctgtgt attaagaatt
     7741 aaatgctctt ccagaaattt tacagggtta ctacgtattt cattcattat acaccgaagc
     7801 tctgaacgat gcggcaccag aaagtgcccg gtattttgtg aaataatact acttaccttg
     7861 attgttttct ctccagctca tgaaaaataa ctcgcgtatt atattgtttt accaaccaca
     7921 ggccccgaat gcctgtggtg ttt
//
LOCUS       DAZFJF010000180         7661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFJF010000180 DAZFJF010000000
VERSION     DAZFJF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.182"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..358
                     /gene="ydjO"
                     /locus_tag="ACHKAU_004515"
                     /pseudo
     CDS             1..358
                     /gene="ydjO"
                     /locus_tag="ACHKAU_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347595.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(362..1753)
                     /gene="tcyP"
                     /locus_tag="ACHKAU_004516"
     CDS             complement(362..1753)
                     /gene="tcyP"
                     /locus_tag="ACHKAU_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO5533444.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1886..2476)
                     /gene="ydjM"
                     /locus_tag="ACHKAU_004517"
     CDS             complement(1886..2476)
                     /gene="ydjM"
                     /locus_tag="ACHKAU_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5533445.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2639..3307)
                     /gene="hxpB"
                     /locus_tag="ACHKAU_004518"
     CDS             complement(2639..3307)
                     /gene="hxpB"
                     /locus_tag="ACHKAU_004518"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO5533446.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3454..3990
                     /gene="yniB"
                     /locus_tag="ACHKAU_004519"
     CDS             3454..3990
                     /gene="yniB"
                     /locus_tag="ACHKAU_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO5533447.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4031..4891)
                     /gene="yniA"
                     /locus_tag="ACHKAU_004520"
     CDS             complement(4031..4891)
                     /gene="yniA"
                     /locus_tag="ACHKAU_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO5533448.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4997..5287)
                     /gene="ghoS"
                     /locus_tag="ACHKAU_004521"
     CDS             complement(4997..5287)
                     /gene="ghoS"
                     /locus_tag="ACHKAU_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5533449.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5388..6317)
                     /gene="pfkB"
                     /locus_tag="ACHKAU_004522"
     CDS             complement(5388..6317)
                     /gene="pfkB"
                     /locus_tag="ACHKAU_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO5533450.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6604..7362
                     /gene="ydiY"
                     /locus_tag="ACHKAU_004523"
     CDS             6604..7362
                     /gene="ydiY"
                     /locus_tag="ACHKAU_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO5533451.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7415..7522)
                     /gene="yniD"
                     /locus_tag="ACHKAU_004524"
     CDS             complement(7415..7522)
                     /gene="yniD"
                     /locus_tag="ACHKAU_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO5533452.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         1976 a         1881 c         1865 g         1939 t
ORIGIN      
        1 cctgccggga gcatttttgc tgcgattcaa aaaaaatgag agtctcctaa agtttccttc
       61 gataatatca atgcttaata tcaatcttct ttttaatcca ctaaatcttc cgggaatggg
      121 aagtggtgtg cttgaagata ttatgagtat tccagatagt tcacttagaa aacgtctcgg
      181 gtatgaggtt ttaagcttct cactacaggc acattcattg agccaggaat gtattgataa
      241 gttggacatt tttttcgctg acgatctgtt taaatatgag tcagtatgca tcgccgcgat
      301 ggaacattta aaaagtaaag caacggcccc tatacaaaac ggaccgttgc cagcataaga
      361 attaacggtg tgccagttcg gcgtcgtctt cgctatccag aatggcttta tcggtttgct
      421 tcagccactg gctggtcagc gtgccagctg tcatcgagcc actaacgttt agcgccgtac
      481 ggcccatgtc gataagcggt tcaacggaga ttaacagcgc caccagggtt actggcaggc
      541 ccatcgcagg cagcacaatc agtgcggcga aagttgcacc accaccgaca ccggcaacgc
      601 ctgcggaact aacggtaaca ataccgacca gcgtcgcaat ccacatcggg tccagcgggt
      661 taatgccaac cgtaggcgca accatcaccg ccagcattgc cggatacaaa ccggcgcagc
      721 cgttctgacc aatggttgca ccgaaagagg cggcgaaact ggcgatggat tcaggaacgc
      781 ccagacgacg cgtttgtgct tccacattca gtgggataga cgcagcactg gaacggctgg
      841 taaaggcaaa cgtcagcaca ggccatacct tacggaagta cttcagcgga ctcacgccat
      901 taatgcccag cagaatgcca tgcactgcaa acataatcag cagaccgagg taggacgcga
      961 caacgaaact tcccagtttg atgatgtctt gcaggttaga acctgcaacc actttggtca
     1021 ttagtgccag aacgccgtaa ggggtcaact gcatgaccag gcgaaccagt ttcatcaccc
     1081 agctttgcag ggtatcgata gcggttaaga cgcgttcacc tttcggcgca tcatccttca
     1141 gcagtttcag cgcagctaca ccgaggaatg cggcaaaaat taccacgctg ataattgacg
     1201 tcggattggc tccggtcaga tcggcaaacg ggtttttcgg gataaaggac aagaccagct
     1261 gcggaacgct caggtcagag actttaccaa catagttact ttcgatggcg ttcagacgtg
     1321 cagtttctgc accaccctga accagacctt cagccgtcaa accaaacagg ttggtgacca
     1381 gcacaccgac cagcgccgca atcagtgtgg taaacaaaag cgtaccgatg gtcagaaaac
     1441 tgattttgcc taactgagat gcgttatgca gacgggcaac cgcgctcaga atagaggcga
     1501 agactaacgg cataacgatc atttgcagca gttgaacata gccgttacca acgatgttaa
     1561 accactgtac agaatcttta agtacctggc tgtcagaacc ataaatggta tgcagggcaa
     1621 ggccaaaaac cacacccatc accagaccca ccagcacttt tttcgccaga ctccactgtt
     1681 tgtggcgggt ctgagccagc gcaaacagca gtacaacgaa caccacgatg ttcgcaatta
     1741 atggaaagtt catccccgtt ctcctgatct tattatttct atcactgcgt agcagtcata
     1801 gtgttggcgc aagattaaca gaactgtgag ctggctctta tattcaaatg gaattgctta
     1861 ttccagaaag gtgatttttg cctttttagt attcaacctg atgcttaata agccgatgaa
     1921 actggatctg caaggtattg atcatttgcg atgaccaacg aaccgcgctg ttttcgggta
     1981 atgaatgggg catccatacc gaccagacaa ataatgccat gcagataaaa cgttccagtt
     2041 ggttgccctt ttggggaacc aggataggca agcggaaacg ccagcgacat ggccagagca
     2101 ggggaacacc ggcgggtgtc agcatatcgg caagtatgtg gcttaaataa cccagcacca
     2161 ttccttgtag cgcatcagcc ggaatgaacc agctttcagg aaccttaagg taaaaggttg
     2221 ccagcagcgc aaataccgcc agcagactgt gggtaaaacc acgatgccca aaagcgcggg
     2281 cgatcggttt tgatatccac tttaatcgct gcccaagaaa cgactttggg tgatcgatgt
     2341 ccggtaacaa acacgtcagg attgcggaag ggacaatatg ccaccagtca ccctgtgcca
     2401 gcacgggcgt cagctcggca tttttggcaa ataccgcaca agcaatagaa aagagaaggt
     2461 gaccttccgc cgtcatgata aaactcacta aactgtcgat acgtacagta taggattttt
     2521 atacagtgca cggaagtggt gacggtgtaa ctctttctct taaattgacg attaatccgt
     2581 cagttgaatg caaattcaga taaagaacca ggggcagcga gatcactgcc cctggtcatt
     2641 aaccgagaag gtcttttgcg gtgagttctg tcagcgatga gagtttgacg tttgctaata
     2701 caaaacgtgg atcattttgc gcttctggcg caggaacgac aatggaacgc atgcgggctg
     2761 ctttagaggc gatcatgcca tttactgaat cttccagcgc tacgcaggtc agtgggtcaa
     2821 cgcccagttt tgctgcgcag tcgagatata cttgcggatg cggcttgctg taaggcagtt
     2881 tttcagccga ggcgagggca tcgaaactgt cgcgtaagtc aaacatggtc aacacttttt
     2941 ccagcatatg tagtggtgac gcggaggcca gtcccaccaa taaaccttgt tctttgcata
     3001 acgcaacggc ttcgcgcacg cctggtaata atggacgtgt ctcttcaacc agtgaaatgg
     3061 cacgggcaat aacccgttct actacttcct gacggcttgg cccattccac ggttgccggg
     3121 cgtaccaaag atcgaccacc atatcgatgc gtaaacctaa ggtgtccggc agctcgttac
     3181 gacgggagat atccaccccc aggcttgcca tcacatccag ttcggctcga tcccataaag
     3241 gttctgagtc gataagtaat ccatccatat caaaaattgc agcaagaatc tgacgcgggg
     3301 ttgacattac aacctctcct ttagagcatt atgtgtaagg tattaagccg tgcttatgat
     3361 taagcgccta tcatatcgcc tttaaagaat gggcgaaaac agatatctac aggtaaacta
     3421 agtgaaacga cgcgtcttaa tcaaggagaa ctcatgacgt atcaacaagc tggacgcatt
     3481 gctgttttga aaaggatttt gggttgggtg atttttatac ctgcattgat ctctacattg
     3541 atttctttgc tgaaatttat gaatacccgg caggaaaacc aggaaggcat taatgcggtc
     3601 atgctcgact ttactcatgt catgatcgat atgatgcagg cgaatacgcc ttttttgaat
     3661 ctgttctggt ataactcccc gacgcccaat tttaatggtg gcgtgaacgt tatgttctgg
     3721 gtgattttca tcctgatttt tgtcggactg gcgttgcagg attccggtgc ccggatgagt
     3781 cgccaggcgc gcttcttgcg ggaaggtgtt gaagatcaac tcattctgga aaaagccaaa
     3841 ggggaagagg ggctaacgcg tgaacaaatt gagtcccgta ttgttgttcc gcaccatact
     3901 attttcctgc agtttttctc actatatatc ctgccggtta tttgtattgc tgcaggttat
     3961 gtgttctttt ctctgcttgg gtttatttaa tcaaaaggcc gcacggagcg gccattatcc
     4021 tcaacccata tcatgctgct aataatctat ccaatgactg ctgagcaata accaaatgct
     4081 gaccgccaaa taatcttgca cgatttaaca gcgtgtagag ttggtaaacc ggttgacgtt
     4141 caaggaaatc ggcaggtagc ggggatactg actgatagcc gtcatagatt tgtggcggct
     4201 gttcagtatg cagcggtaac atcgccaggt cgcactctcg gtcaccccag tagcaggcag
     4261 ggtcgaaaat gtacgggcca tccggaccca gcgcacagtt gccggaccat aaatcgccgt
     4321 gcaacagaga aggctgcggt tgatgcgagg ccagacgctg ctgaatatgc tcgacgatgg
     4381 cgtcgatatt gccgaaagcg atccctttct ctgctgccag ttccaactgc cagccaatcc
     4441 gttgttcagc aaaaaacgtt gaccagcgac gttgccaggt gttgggctgt ggagttgtgg
     4501 agagcgagtt atcaaaatcg aggccaaatt gtggttggtc actccattga tgtaaacgcg
     4561 caatttgctg accaagaata aatgcgctat gcgcatccag cggacgaggt gggagataat
     4621 ccatcaccag aaaactgtag tcacggtcag cgcctactgc ccaaacctta ggcacggtga
     4681 cggttttact acgcgacagt aactccagtt ggtcggcttc ggcggtaaaa ccgggaagca
     4741 gttccctttc atcacatttg acgaaaaagt catgtcctgc atagcgcaaa tgccatgcgg
     4801 catggacttc tccgccaggc agttcattac gcagttcgat ttcgccttca cctaactgct
     4861 cgctcaaaag acgactgatt gcctgccaca tgatgtctct cccatgttgt ctgccagatc
     4921 ataaggttag cgcaagaatg cggtgtaaaa acacgagcta acgcacacca cagactgatt
     4981 attgcccaaa aaccatttat tttctgttgc tattccattc ctcccaggtc gtgatggtga
     5041 tttcaggatc tttgccggtt gcactttggg taagccccga aaccagttct ctaatttctt
     5101 cttcactttg ggtactgata agtccaaaag tgttagtccc cagctcatgg atatttccct
     5161 catcatccgt catggtgagc aaaaaaccgt cgcgagtcag gtaattattc aactcgttaa
     5221 tttcagtcaa tgtatcctca tgcaacatga cggttatgac ataacggaca agatcgccac
     5281 tggccatagt tcaccccttt gttatcacat gacttttgag catagtcgga gaaacgcgtt
     5341 gccgacaggt tggtggtgat tcccccaatg ctgggggaat gtttttgtta gcgggaaagg
     5401 taagcgtaaa ttttttgcgt atcgtcatgg gagcacagac gtgttccctg attgagtgtg
     5461 gctgcactcc ccgcagctac gccaaaacgg accatctctt caagagaggc attttctgcc
     5521 agtttcagtg tcatcgcgcc gaccatgctg tcaccagcgc caacggtact ctggcttttc
     5581 accggtggtg gcaccacctg aatgcagttt tcactatcaa cacccagcgc tccttgtgga
     5641 ccaagggaaa cgacaacccg tttggccttg ccgctattaa cgatttcctg cgcggctttg
     5701 cggacatcgt ctggctgggt gagttcgcga ttcaccagcg cactgagttc tttttggtta
     5761 ggcttaacca actcgatgtt accaatcgcc agtgctgcac ttaacgcttc gccagaactg
     5821 tcgatgatgc agcggatccc ttggttttgc gcagcggaaa tcagttgggt taatttttcc
     5881 agcttcacac ctggcggcag gcttccgctt atgaccagga tggccccgga ttcaatttcc
     5941 agaacttgct cttcaagctg gcgaaactca tcttcattta atgccgcgcc tggcataaca
     6001 aaacgatact gctcaccgct tgcttccaca tgtacgtgta aattctgccg ggtccagtct
     6061 ttggcttcta cagtagcgac ggggacattt tcatccgcca acagagaaac caggtgttcg
     6121 ccggtcgcgc cacccgccgg gaagatcgct gtggcactgc ctccaagatg ggcaatggcg
     6181 cgggcgacgt tgatgccgcc gcccccgggt tcgaacaccg gtgcggtaca gcgcagtttt
     6241 ccttcgggat aaatttgcgg ggtaattgtt gcgctatcga gagagggcgc aagtgtcaac
     6301 gtatagatac gtaccatcat ttcctcctat aggctgattt cagtctggca ccgaatcagc
     6361 cgaaagtgaa gaaattaaca atatgattta ttagagcttt atttaaaatt ttgcagataa
     6421 atatatataa ataaaaatct gttttcgcca catgcttatt caaaaaatga aataagaata
     6481 cattgctaag ttattcgcgc ctttttataa ttcgttacct ttccagggac gctaatcttt
     6541 gatccttaag aaagtttccg ggaccgcgat ggtctctttt tttgttgtac ggactcttga
     6601 taaatgaagc ttttgaagac agttcctgcc atcgttatgt tggcgggggg catgtttgcg
     6661 tcactgaatg cagctgccga tgattccgtt tttactgtca tggacgatcc tgccagcgct
     6721 aaaaaacctt tcgaaggtaa tctgaacgca ggatatcttg cacaatcagg caatacgaaa
     6781 agctcctcac ttacggctga caccaccatg acctggtatg gacaaaccac tgcctggtcg
     6841 ctgtggggga acgccagtaa tacttcttct aatgatgagc gttcttcaga aaaatatgcg
     6901 gcgggcgggc gtagccgttt taatttaact gattatgact atttatttgg tcaggcaagc
     6961 tggctgacag accgttataa cggctatcga gagcgcgatg tgttgaccgc gggttatggt
     7021 catcagtttt tgaatggccc ggtacacagt ttccgctttg aattcggtcc aggtgtgcgt
     7081 tacgacaaat ataccgacaa tgccagtgaa acccagccgc tgggctatgc ctccggggct
     7141 tatgcatggc agttgactga caacgctaaa tttacgcagg gtgtttcggt ctttggcgcg
     7201 gaagacacaa cgctgaactc cgaaagcgca ttaaatgttg ctattaatga acattttgga
     7261 ctgaaggtgg cttacaacgt cacatggaac tctgaacctc cagagtctgc accggaacat
     7321 accgaccgtc ggacaacctt atcgctgggc tacagcatgt aaaattatct gccggaccgt
     7381 gttatgcggt ctggctgtct tcgtttgtca ctgctcagcc ccaaatcatt gctgcccaac
     7441 gtagcaacaa catagcgcca cagattgcca gtagcaccac caccatcttc cagtgttttt
     7501 tatcaaagcg tttagtcggc ataccagtgt cctgcatatc tcttgtgcgt gataacggag
     7561 tgtaacaaaa gggacgggca tcaagggatt ttttgttatt acagccgttg ggtggcgttg
     7621 tacacctggt cactgtttct gcgttttatg aaaaaaataa a
//
LOCUS       DAZFJF010000181         7648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFJF010000181 DAZFJF010000000
VERSION     DAZFJF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.183"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1433
                     /locus_tag="ACHKAU_004525"
                     /pseudo
     CDS             1..1433
                     /locus_tag="ACHKAU_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1469..1672)
                     /gene="ydfZ"
                     /locus_tag="ACHKAU_004526"
     CDS             complement(1469..1672)
                     /gene="ydfZ"
                     /locus_tag="ACHKAU_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO5533453.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1850..2536)
                     /gene="rspR"
                     /locus_tag="ACHKAU_004527"
                     /gene_synonym="ydfH"
     CDS             complement(1850..2536)
                     /gene="rspR"
                     /locus_tag="ACHKAU_004527"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO5533454.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2625..3371)
                     /gene="ydfG"
                     /locus_tag="ACHKAU_004528"
     CDS             complement(2625..3371)
                     /gene="ydfG"
                     /locus_tag="ACHKAU_004528"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO5533455.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3508..5553
                     /gene="dcp"
                     /locus_tag="ACHKAU_004529"
     CDS             3508..5553
                     /gene="dcp"
                     /locus_tag="ACHKAU_004529"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO5533456.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5598..6116)
                     /locus_tag="ACHKAU_004530"
     CDS             complement(5598..6116)
                     /locus_tag="ACHKAU_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO5533457.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            6392..6784
                     /gene="ydeI"
                     /locus_tag="ACHKAU_004531"
     CDS             6392..6784
                     /gene="ydeI"
                     /locus_tag="ACHKAU_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO5533458.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            7039..>7648
                     /gene="dgcZ"
                     /locus_tag="ACHKAU_004532"
     CDS             7039..>7648
                     /gene="dgcZ"
                     /locus_tag="ACHKAU_004532"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO5533459.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVY"
BASE COUNT         1942 a         1755 c         1918 g         2033 t
ORIGIN      
        1 cgacactccc tgtttatgat cgtaataacc tggccccaag aattattcat ttaggctttg
       61 gtgcatttca ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca gaacatttca
      121 gtgactgggg atattatgag gtcaacttaa tcggcggcga acagcaaatt gccgatttac
      181 aacagcaaga taatctttat accgttgcgg aaatgtcggc cgatgcgtgg acggctcgcg
      241 tcgttggcgt cgttaaaaaa gccttgcacg tacagatgga tggcttagaa accgtgttgg
      301 ctgcgatgtg tgaaccgtaa atcgcgattg tctctctgac aatcaccgaa aaagggtatt
      361 tccactctcc ggcgatcgga cagttaatgc tcgatcaccc gatggtcgct gccgacgtgc
      421 aaaatcccca ccagccgaaa acagcaacag gagtgattgt tgaggcgctg gcccgccgta
      481 aagcggcagg acttcccgca tttaccgtca tgtcatgtga caacatgcca gaaaacggtc
      541 atgttatgcg tgacgttgtc acttcatacg cgaaagctgt tgatgtaaaa ctggcacaat
      601 ggatcgaaga taacgtgact ttcccatcaa caatggtgga ccgtattgtg cccgcagtga
      661 cagaggatac gctggcgaaa atcgaacaac ttaccggtgt gggcgatcct gcgggcgttg
      721 cctgtgaacc tttccgccag tgggtaatag aagataactt tgttgccgga cgtccagaat
      781 gggaaaaagc gggagccgaa ctggttagtg atgtgctgcc ttatgaagag atgaagttgc
      841 gcatgctcaa cggcagtcat tcattcctgg cgtatttggg ttatcttgct ggatatcagc
      901 acattaatga ctgcatggaa gatgaacatt atcgttatgc ggcgtatggc ttgatgttgc
      961 aggaacaagc gccgacgctg aaagtgcagg gcgttgattt gcaagattac gctaaccgat
     1021 taattgcacg ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg
     1081 gtagccagaa attgccacag cggatgttgg attctgttcg ctggcatctg gcgcatgaca
     1141 gcaagttcga tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc ggtggtgttg
     1201 atgaacaggg aaatctgata gaaatcagtg acccactgtt acctgttatt cagaaggctg
     1261 tacaaagtag tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcaatct
     1321 ttggtgatga tttgccagac aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt
     1381 ctttattagc gcatggcgcg aaagcgaccg tggcgaaata ttccgtgaag taaaaaaagc
     1441 aatgccgcca tcggcggcat tcacacactt agttcatgcc gagacgaatc aggtccagag
     1501 gtgccagttt ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag
     1561 cggtcagacc ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca
     1621 taacacggct gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt
     1681 tctcctttct gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg
     1741 agtgaaatat tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccgt
     1801 aaggcggggg gaaaagcctc tcatcccgga tgggatgaga gtaaatgaat tactcttcgc
     1861 taaaccagtc gctgttttcc tggcggatct ggcgcacgga ttcgctgatt tcctgcaaat
     1921 gttgcgtcat tgcacgttct accgcatcgc catcacgttt ttgcagggca gagaaaatat
     1981 caagatgctg gcgtaacagc atttctggtg gagaaacatg gtcgaaactc atatagcgca
     2041 cgcgatcaac ggtcgctttc aggttctcaa tggtatccca cgccagttga cagtcggcaa
     2101 tctgcgtcag gagttgatgg aagttgtcat caagttcaaa aaaatcatcc agttgcttgc
     2161 gctcaatggc aatgcgttgc tggtgaagat tttgttccag ttggtagcac tggctttcgg
     2221 taatcatgct cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg ataaaactgc
     2281 cgtttcgcac ctgggccatg gaaattttgt tgacgtagct gccacgctgc ggacgaattt
     2341 gaatcaggcc gttttccgcc agtttaataa aggcttcacg aaccggctgg cgtgacacat
     2401 tgaaacgaac agaaacttct ttttccgaca acggtgtgcc tggtgcaatc aggcaatgga
     2461 caatgtcgcg acgaagaata cgataaatct gctgattgac aggctgtgtg ggattaagtt
     2521 gcgtttcgac ggtcattcgt tcttgcttgt gagtgagtta actgcatgag tctaccactt
     2581 ttttgtgaca agcaataacc cggcagttac gccgggtata aaaattactg acggtggaca
     2641 ttcagtccgg catagctttg ggtaaccggc atcatttcca gggtattgat attgacgtga
     2701 gcaggcagcg ttgacaccca ccagacggct tcgctgacat cttctggcgt caatgcaacg
     2761 gtattttgat aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac
     2821 tcggtgccac ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcagg
     2881 tccgtacgca gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta
     2941 ccaccggcat aaggccagct acctgccgtt gagccaatgt taataatatg accatgatta
     3001 cgttcaacca tacccggtaa gacggcgcgc gtcatatata ccaggccttt gttgttggta
     3061 tcaatcatcg tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc
     3121 aggccggcat tatttaccag gatatcaata ttgcaccact cggcagggag cgatgccagc
     3181 atctcttcaa tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc
     3241 agttcgtctt ttaactcctg caaccgctcc tggcgacggc cagtggcgat aactttatgc
     3301 ccttgttgaa taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact
     3361 aaaacgatca tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc
     3421 ttaagagcgg gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat
     3481 ggaacacctc gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc
     3541 acactgccgt atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca
     3601 ttcgatgagg gaatgcagca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa
     3661 acgcctgatt tcaacaatac tattctggca ctggaacaaa gcggagaatt acttacccgc
     3721 gttaccagcg tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt
     3781 gatgaacagt tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa
     3841 ttattcgcgc gggtagatgc tgtctggcag cgccgtgaat ccctggggct tgatagtgaa
     3901 tccatccgcc tggtggaggt gattcatcaa cgttttgtcc ttgccggagc caaacttgcg
     3961 caagctgata aagcaaaatt aaaagtactg aatacagaag ctgcgacact gaccagtcag
     4021 tttaaccagc ggttactggc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg
     4081 cagctggcag gaatgagtga gcaagagatt gcgctggcgg cagaggcggc tcgcgagaaa
     4141 ggtctggata acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc
     4201 gaaatgcgcg atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa
     4261 aaaaatgatg gcaatgatac ccgcgctatc attcaacgtc tggtggagat ccgtgcacaa
     4321 caggcgacat tacttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca
     4381 aaaacacctg aagcagcgct caactttatg cgggaaattg ttccagcggc gcgtcaacgt
     4441 gcgagcgatg aattagcctc catacaggcg gttattgata agcagcaggg cgggtttagc
     4501 gcgcagccgt gggactgggc attttatgcc gaacaggtac ggcgggagaa atttgatctt
     4561 gatgaggcgc agctcaagcc atattttgaa ttaaacacgg tgttgaatga aggtgtattc
     4621 tggaccgcga atcagctctt cggtattaag tttgtcgaac gttttgatat tcctgtctac
     4681 catcctgacg ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt
     4741 tacggtgatt tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt
     4801 gagcaatcaa cgcttaatga aacgcattcg gtaatttata acgtttgcaa ttatcagaaa
     4861 cccgctgccg gtgagcctgc gttgttactc tgggatgatg tcataacctt attccatgaa
     4921 tttggtcata cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc
     4981 aacacgccgc gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat
     5041 ccgcaggtat tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa
     5101 ctgcaacaga aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg
     5161 cttagcgccg cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag
     5221 gatgtcgatg attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata
     5281 ccgccacgct atcgcagcag ttatttcgcc catatttttg gtggcggata tgccgcaggt
     5341 tattacgctt atctgtggac acaaatgttg gccgatgatg gttatcagtg gtttgttgag
     5401 cagggcggat taacgcgtga aaatgggctg cgttttcgcg aggcgatcct ttccagaggt
     5461 aacagcgaag atctggaacg cctgtatcga caatggcgcg gtaaggctcc tcagattatg
     5521 ccgatgctgc aacatcgtgg cttgaacata taaacaccac caatggctgt cctggagata
     5581 tcgccaggcc agcctggtta tagcagtaat tgcagcagct gggcgagggc aaacctcacc
     5641 gctaaagcta ataccgtttc gcagtcgcca gcaaaatgca taaccgcagt ataggtctgg
     5701 cctttaatat gccacgcgaa ccagacggta cccgctggcg taccatcttc accgccctcc
     5761 ggtccgccgt agccggtaat ggcaatactt acatcagcat ccgcacgctc tatggctccg
     5821 gttgccattt ctgctgccac tttctcactc accgcagaat atcgttcaag agattgctgg
     5881 cttacgctga ggattttcat ctttgcctga tcggtgaaag taacaaagcc tgcaccgtaa
     5941 aatttgggtg tatcttcagc tgcacacagg gcgctagcca acttaccgcc ggtgcatgat
     6001 tcggcggtag ttaagcgtaa ttgatccgcg ataagtcttt gactcaacgc ggatgtcagg
     6061 ttttcaatag tgtcggtatc tgcgagttgt acaattttat ctttattaat gttcactgtt
     6121 ttttgtccac gctattatat gtaaaggtga tttgcttttt ctgtatgctc gtcaaatata
     6181 ataattttca aacatataaa tccgattatt ctgaaaatat gtttaagact cttcaatctt
     6241 cttaacttta gtgagttaaa ttctctgaat atttgacagc tagtgggctg attttaaatg
     6301 ccttataaca attggcagta ttcttatttg gcaattataa aaatagcaac cagacttaat
     6361 aagtcaaacc aaacgcaatt aaggagttag tatgaaattt caggccattg tattagcaag
     6421 ttttcttgtc atgccttatg cgttggcaga tgaccagggc ggtttaaaac aggatgcagc
     6481 gccaccgccg cctcatgcaa tagaagatgg ctatcgcgga accgatgatg caaaaaaaat
     6541 gaccgttgat ttcgcaaaaa acatgcacga tggcgcttcg gtttcactac gcggtaattt
     6601 gatttctcac aaaggagagg accgttacgt ttttcgcgat aagagcggtg aaattaatgt
     6661 cgttattcct gcggccgtct ttgatggacg agaagttcag ccggaccaga tgatcaacat
     6721 tagcggcagt ctggataaga aaagcgcgcc ggccgtcgtt cgggtcactc atttgcagaa
     6781 ataaaaataa attagcctga tggcctgaac tactgggcca ttagggtatt gggttatgct
     6841 atttgtcatc cttttattta cgataatgca gaggtgattt acagaattaa acactttttt
     6901 atccgctgat tttgttttaa atcacaatgt tatctcagag atgactcata acttcggatt
     6961 tctatgaaaa tattgacgta attttgcttt tctctcgtta gaatagcgcg cacaaggaac
     7021 tgtgaaaaag gagtggcaat gatcaagaaa acaacggaaa ttgatgccat cttgttaaat
     7081 ctcaataagg ctatcgatgc ccactaccag tggctggtga gtatgtttca cagcgtggtc
     7141 gcgagagatg ccagtaagcc agaaataacg gataaccatt cttatggact gtgccagttt
     7201 ggtcggtgga ttgatcatct ggggccactc gataacgatg aattacctta cgttcggcta
     7261 atggattctg cccatcaaca tatgcataac tgtggtcggg aattaatgct ggctattgtt
     7321 gaaaatcact ggcaggacgc gcatttcgac tcctttcagg aggggttgct ttcttttact
     7381 gcggcattaa ccgattacaa aatttatttg ctgacgatcc gtagcaatat ggatgttttg
     7441 acgggattgc cggggcgtcg ggttcttgat gaatcctttg atcatcagtt acgcaacact
     7501 gagcctctga atctttattt aatgttgttg gatattgacc gatttaaatt ggttaatgat
     7561 acctacgggc atttaatcgg cgatgtagta ttacgcaccc tggcaactta cttagccagt
     7621 tggacgcgtg attacgaaac ggtttatc
//
LOCUS       DAZFJF010000182         7611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFJF010000182 DAZFJF010000000
VERSION     DAZFJF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.184"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..1506
                     /locus_tag="ACHKAU_004533"
     CDS             76..1506
                     /locus_tag="ACHKAU_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5533460.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPFLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTASAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(1507..2415)
                     /locus_tag="ACHKAU_004534"
     CDS             complement(1507..2415)
                     /locus_tag="ACHKAU_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5533461.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            2515..3105
                     /locus_tag="ACHKAU_004535"
     CDS             2515..3105
                     /locus_tag="ACHKAU_004535"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO5533462.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(3187..3927)
                     /gene="pflA"
                     /locus_tag="ACHKAU_004536"
     CDS             complement(3187..3927)
                     /gene="pflA"
                     /locus_tag="ACHKAU_004536"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO5533463.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4119..6401)
                     /gene="pflB"
                     /locus_tag="ACHKAU_004537"
     CDS             complement(4119..6401)
                     /gene="pflB"
                     /locus_tag="ACHKAU_004537"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5533464.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(6456..7313)
                     /gene="focA"
                     /locus_tag="ACHKAU_004538"
     CDS             complement(6456..7313)
                     /gene="focA"
                     /locus_tag="ACHKAU_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO5533465.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1947 a         1746 c         1871 g         2047 t
ORIGIN      
        1 ttccgcactt cttatttgcc gcgcataatc cctcgtttta cagatgcccc tttaattttg
       61 gcgaaggatt tgtctatggc tgggaatgtt caggaaaaac agttgcgatg gtacaacatt
      121 gcgctgatgt cttttatcac tgtctggggt tttggcaacg ttgttaataa ctatgccaac
      181 caggggctgg tggttgtttt ttcatgggtg tttatctttg cactctattt cacaccttat
      241 gcgctaattg ttggtcagtt aggctcgacc ttcaaagatg ggaagggcgg ggtcagtacc
      301 tggattaaac acacgatggg acccggactg gcttatctcg ccgcgtggac ctactgggtg
      361 gtgcatattc cctttctggc acaaaaaccc caggcaattc tgattgcgct cggttgggcg
      421 atgaaaggcg acggttcgct aatcaaagaa tattcagtcg tagcgttaca ggggttaacg
      481 ctggtgctgt ttatcttctt tatgtgggtt gcttcacgcg gtatgaaatc gctgaaaatc
      541 gtcggttctg tggcagggat tgcgatgttt gttatgtcgc tcctgtatgt ggcgatggcg
      601 gtaaccgcgt ctgcgattac tgaagtgcat attgcgacca caaacattac ctgggaaacg
      661 ttcattcctc atatcgactt tacctacatt accactattt caatgctggt tttcgcggtt
      721 ggcggagcag agaagatttc tccttacgtt aatcaaacgc gcaacccagg aaaagaattt
      781 ccaaaaggga tgctatgcct ggcggtgatg gttgcggttt gtgccattct gggctcactg
      841 gcgatgggga tgatgtttga ctcgcgtaat atcccggatg acttaatgac taacggtcaa
      901 tattacgcct ttcagaagct gggcgagtat tacaacatgg gtaatacttt aatggtgatt
      961 tacgccattg cgaataccct gggacaagta gctgcgctgg tattctcgat tgatgccccg
     1021 cttaaagtgc tattaggtga tgctgacagc aaatatattc cagccagttt atgtcgtacc
     1081 aacgcttctg gtacgcccgt taatggctat tttctgaccc tggtgctggt ggcaattctg
     1141 attatgctgc cgactctcgg catcggtgat atgaacaatc tctataaatg gctgttgaat
     1201 cttaactcgg tagtgatgcc gctgcgttat ttatgggtat ttgttgcatt tattgcagtc
     1261 gttcgcctgg cgcagaaata taaaccagaa tatgtcttta ttcgtaataa gcctctggca
     1321 atgaccgtcg ggatttggtg ttttgccttt accgcctttg cctgtttgac ggggatcttc
     1381 ccgaaaatgg aagccttcac tgcagagtgg accttccagt tggcgctgaa tgttgcaacg
     1441 ccgtttgtgc tggtaggatt aggactgatt ttcccgctgc tggcgcgtaa agcgaatagt
     1501 aaataattat tgtggtcggt gacgctgact ctggcagatc tgacccgttt ttacataatc
     1561 gagaaatgcg cgcaagccgc aggacatata ctggcggttt ggataataga tctggaagcc
     1621 tggacgctct gtgctccagt ctcccagtac acattctaaa cgaccagtat caaggtactc
     1681 cttgatctgt tcatagagca gatacccgat accagcaccc atcaaaacgg cctccagttc
     1741 agcatcaaca tcatccagaa tgatatttcc tgcgacagca atttccagct cctttgcaaa
     1801 ttgccagtgg aaaggtttgc cgctgggata acgaaaaaca acacattgat gattaagcag
     1861 atcatgagga tggcggggtt tgccatatct tgcaaagtac tctggcgttg ctgctacaca
     1921 taatttcact ggtgggccga tcgccacgct gatcatatct ttttcgacga tgccactaag
     1981 tcgaacgcca gcgtcaaagc cctgttggac gatatcgaca agagaatcgt cagttgtgag
     2041 ctcgacttta atatcaggat attcacgtgt gaaaccaacc agtaaaggca ttaaaaatat
     2101 acgtgcagct acccgggcgg cattgatttt gagcgttcct gtcggtgtca gacgaaaatc
     2161 attcatctca tctaacatga tctgaatttc gtcaaaagct gggcgcaatc gttcatagag
     2221 attagaaccc gcttccgtta acgatacgct tcgggttgtc cgattgaata agcgaatttt
     2281 aagacgttgt tccagtgttt taatactatg gctaatggcg gacgaggata agcctaactc
     2341 atcgcccgct gcacgaaagc tttgattacg ggcgacggca aagaaagtgg caaagtcaga
     2401 catattcatc cgcattagtg aaaatccttc attaactcat cctgaatcat cacgtttaca
     2461 ggattatgcc gcctgcgtaa agtgcgctcc agaacttaac gtggaggtaa aattatgcag
     2521 tctgaacgta tttatttggt atgggcccat cctcgtcatg attcattgac cgcaaatatt
     2581 gctgatgcga tccatcagcg ggcaatggag cggaaaatac aggtgacgga actcgattta
     2641 tatcggcgta atttcaaccc agtgatgacg ccggaagatg aaccagactg gaagaatatg
     2701 gataaacgtt attctccaga ggttcatcag ctttattcag agctgcttga acatgacacg
     2761 ttagtggtgg tttttcctct ctggtggtat agcttcccgg caatgctaaa aggatatatt
     2821 gacagagtat ggaataatgg gctggcttat ggagatgggc acaaattacc attcaataaa
     2881 gttcgttggg tggcgctggt tggaggagac aaagaatcat ttgtccagat gggctgggaa
     2941 aaaaatataa gcgattattt gaaaaatatg tgcagttatc ttggtattga agatgccgat
     3001 gtcactttct tgtgtaatac agtggtattc gatgggcaag aacttcatgc gagctattat
     3061 cagtcgttat tatctcaggt acgggatatg gtagatgcac tataaaatgt gttaaaaacg
     3121 ctgtagcaga atgaagcgcg gaataaaaaa gcggcaactc aataaagttg ccgctttacg
     3181 gggaaattag aacattacct tatgaccgta ctgctcaaga atgcctttca cgcgttccat
     3241 ggtctctttc ttaggtggtt taacaccgtc gagtttgtac tcttcaccca ttgccaccca
     3301 tttgtgtttg cccagctcgt ggtaggggag gagttcgatt ttctcaacgt tgcccatatc
     3361 acgggtaaat tcaccgaggc gatgcgctga atcgtcatcg tcagaccagc ctgggacaac
     3421 aacgtagcgg atccacacct tcacattttt gttcgccaga tatttggcga actccagcgt
     3481 gcggtggttg gaaactccaa ccagattttg gtggatctcg tcgttcatct gtttgagatc
     3541 gagcattacc aggtcggtta cttccagcag ttcatcaatc accggatcgt aacgacgaac
     3601 aaaaccgttg gtgtccagac aggtatgaat gccttctttt ttgcaggcgc ggaaccagtc
     3661 acgaacaaac tcagcttgta ggattgcctc accgccggat gcggtaacgc cgccgccgga
     3721 agcgttcata aagtggcgat aggtcaccac ctctttcatt aaatcttcaa cggtaacttc
     3781 tttaccgcca tgcgtatccc aggtgtcgcg gttatgacaa tacaggcagc gcatcaggca
     3841 gccctggaaa aaggtgataa agcgaatacc tgggccgtct acggttccac aggattcaaa
     3901 ggagtgaatg cgaccaataa ctgacattgc ggtgtttctc cagatgtggc ccatctgagg
     3961 ccgtgttggt gcgcagctcg aaggctacgt cgagtctgtt ttggcagtca ccttaaagta
     4021 tagatagctg acaaaaaagg ctctcgtgct aaaaaaggcc ccactttcgt ggagccttta
     4081 ttgtatgctt tttactgtac gatttcagtc aaatctaatt acatagattg agtgaaggta
     4141 cgagtaataa cgtcctgctg ctgttcttta gtcagcgagt tgaaacgtac tgcgtagcca
     4201 gatacacgga tggtcagctg cggatatttt tccgggtttt ccatcgcgtc gagcagcatt
     4261 tcacggttca tcacgttaac gttcaggtgc tgaccacctt cgatggatgc ttcgtggtgg
     4321 aagtaaccat ccatcagacc agccaggttg gtcttacgaa cttcgtcgtc tttacccagt
     4381 gcgttcggaa cgatagagaa ggtgtaggag ataccatctt tagcgtaagc aaacggcagt
     4441 ttagcaacgg aagtcagaga cgctacagca cctttctggt cacgaccgtg catcgggtta
     4501 gcacccggtc cgaacggcgc gccagcacga cgaccgtctg gggtgttacc agttttctta
     4561 ccatacacaa cgttagaagt gatggtcaga acagactgag tcgggatagc gtcacggtag
     4621 gtgtgcagtt tctgaatttt cttcatgaaa cgttctacca ggtcaacagc caggtcatct
     4681 acacgcggat cattgttacc aaactgcggg tattcgcctt cgatttcgaa gtcgatagcc
     4741 agaccgtctt cgtcacgaat cggtttaact ttcgcatatt tgattgcaga cagggagtca
     4801 gcagcaacgg acagaccagc gataccacac gccatggtgc ggataacgtc acggtcgtgc
     4861 agcgccatca gagaggcttc gtagctgtac ttgtcgtgca tgtagtggat gatgttcagt
     4921 gcagtgatgt actgtttagc cagccagtcc atgaagtgat ccatgcgctc catcacttca
     4981 tcatagttca ggacatcgcc tttgatcggt tcagacttcg gaccaacctg cattttcagt
     5041 ttttcgtcaa cgccgccgtt gattgcgtac agcatggttt tcgccaggtt tgcacgcgca
     5101 ccgaagaact gcatttgttt accaacgatc atcgggctta cgcagcaagc aatagcgtag
     5161 tcatcgttgt tgaagtccgg acgcatcagg tcatcgttct catactgcag agaagaggtg
     5221 tcgatggaca ctttagcggc gaatttcttg aagttcagcg gcagtttttc agaccacaga
     5281 atggtcatgt tcggttccgg agacggaccc atggtgtaca gggtgttcag gaaacggaag
     5341 ctgtttttgg taaccagggt acgaccgtcg aggcccatac caccgataga ttcggttgcc
     5401 cagatcgggt cgccagagaa cagttcatcg tattccggag tacgcaggaa gcgaaccata
     5461 cgcagtttca tgaccaggtg gtcaaccatt tcctgcgctt cttgttcggt gatcttgcca
     5521 gctttcaggt cacgttcgat gtacacatcc aggaaggtgg aggtacgacc gaaggacatt
     5581 gcagcaccgt tctgagactt aacagcagcc aggtagccga agtaagtcca ctggatagct
     5641 tcctgagcgt tggtagccgg accagagatg tcgtagccgt atttcgcagc catttctttc
     5701 atctgaccca gagcgcggtg ctgttcagcg atttcttcgc gcagacggat agtctgttcc
     5761 aggtttacgc cgttttccag atcagcctgc agagaagtga actgtgccag tttgtctttc
     5821 atcaggtagt cgataccgta cagcgcaacg cgacggtagt caccgatgat acggccacgg
     5881 ccatatgcat ctggcagacc ggtcagaaca ccagatttac ggcaacgcag gatgtccgga
     5941 gtgtaaacgt cgaacacgcc ctggttgtga gttttacggt attcagtgaa gatttttttg
     6001 atcatcggat ccagttcgcg gttgtacgct ttgcaggaac cttcgatcat tttgatacca
     6061 ccgaacggga taagagcacg tttcagcgga gcttcagtct gcagaccaac gattttctca
     6121 agctgcttgt tgatgtagcc agcgtcgtga gaggtgatgg tggaagcaac agcggtgtca
     6181 aagtcaactg gcgcgtgagt gcggttttcc agtttaacgc cttccattac tttgtcccac
     6241 agggtggtgg tcgcttcagt agcgccagcc aggaaggact cgtcaccctc gtacggagtg
     6301 tagtttttct gaatgaagtc acggacgttt acttcattct gccagtcacc tttggtaaaa
     6361 ccttcccagg ctgtggctaa cttttcatta agctcggaca tgtaacacct accttcttaa
     6421 gtggattttt tatttactgc gtacttcgac aaccattaat ggtgatcgtt ttcacgcagg
     6481 taaatgaccc agtatgtcaa cccaaccaac aaaccaccgc cgataatatt accgatcgta
     6541 accggaatca ggttatcagt gatgaagttc atcacggtca ggtgagaaaa attttccggt
     6601 gcagaaccga cagcggtcca gaattccgga gatgcgaagt cgcggattac aatacccatc
     6661 gggatcataa acatgtttgc gatactgtgc tcaaaaccgc tggcaacaaa catcgcgacc
     6721 ggcagcacca taatgaacgc tttgtccatc aggctacggc cagaataact catccatact
     6781 gccagacata ccatcaggtt tgccaggata ccaaggcaga cggcctcaat aaaagtatgg
     6841 tgcactttgt ggtcggcggt ttgtaggacg tttagtcccc attgaccatt tgcggtcata
     6901 tactcgccgg aaagccacat taaaagtaca aacagcagtg cgccgaccag gttgccaaaa
     6961 tagacattta gccagttttt cgccaactga ccccaggtga tgcgcccact cgccttagca
     7021 acaacaatca acactgtgga agtaaagaga tcggctccgc agacaacaca aagaatcagc
     7081 cccagagaga agcaaatgcc gccaaccagt tttgccatgc cgaagggcat tgtgcctgtg
     7141 ccagtggttg ctgtgatata gaagacgaat gcgattgaga tgaaaacacc ggcggtaatc
     7201 gccagataga aagtcttaag cggatgtttc gttgctttat agacacccgc ctcttcggcc
     7261 actttggcca ttgcagcagg aagtaaaaga tcaaaagggt tgtcagcttt cacactaact
     7321 ctctctttat taagtcggcg acgagatact aacaaagcat tatagatgag aaattgatat
     7381 agatcatatc tcgcctggct tataggcccg taactcgcat ggtttttatg caaatacgga
     7441 gtaaatattt gattatccaa ataaaaataa attttaaaaa ttaacaaatg agttgaattt
     7501 tttccgcatc ctccgctaaa acagttaatt aaaagggagc atcaggcgaa taaagtaaca
     7561 atatcgatcg tatttattaa atacaaatta ccgatattta accttataat t
//
LOCUS       DAZFJF010000183         7602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFJF010000183 DAZFJF010000000
VERSION     DAZFJF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.185"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            104..646
                     /gene="ecpR"
                     /locus_tag="ACHKAU_004539"
                     /gene_synonym="matA"
     CDS             104..646
                     /gene="ecpR"
                     /locus_tag="ACHKAU_004539"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO5533466.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            720..1307
                     /gene="ecpA"
                     /locus_tag="ACHKAU_004540"
                     /gene_synonym="matB"
     CDS             720..1307
                     /gene="ecpA"
                     /locus_tag="ACHKAU_004540"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO5533467.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1365..2033
                     /gene="ecpB"
                     /locus_tag="ACHKAU_004541"
                     /gene_synonym="yagY"
     CDS             1365..2033
                     /gene="ecpB"
                     /locus_tag="ACHKAU_004541"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO5533468.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2059..4584
                     /gene="ecpC"
                     /locus_tag="ACHKAU_004542"
                     /gene_synonym="yagX"
     CDS             2059..4584
                     /gene="ecpC"
                     /locus_tag="ACHKAU_004542"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO5533469.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            4574..6217
                     /gene="ecpD"
                     /locus_tag="ACHKAU_004543"
     CDS             4574..6217
                     /gene="ecpD"
                     /locus_tag="ACHKAU_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO5533470.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6186..6896
                     /gene="ecpE"
                     /locus_tag="ACHKAU_004544"
     CDS             6186..6896
                     /gene="ecpE"
                     /locus_tag="ACHKAU_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO5533471.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7209..7538
                     /gene="ykgJ"
                     /locus_tag="ACHKAU_004545"
     CDS             7209..7538
                     /gene="ykgJ"
                     /locus_tag="ACHKAU_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5533472.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
BASE COUNT         1967 a         1949 c         1986 g         1700 t
ORIGIN      
        1 tccggtttac taacgaaact gaatgcacct gtaaaaatta caggtttgga aagtagtgac
       61 atggcaaagt gattacagca gggactatga ggttaaaaac catatggaat gtcaaaaccg
      121 ttctgataaa tacatctggt ctccccatga cgcctacttc tataaaggac tatctgaact
      181 gattgtggat atcgacagat taatttatct atcgttggag aaaattagaa aagatttcgt
      241 gtttatcaat ctcaatacgg attctttatc tgaatttata aaccgtgata atgaatggtt
      301 atccgcggta aaggggaaac aggtcgtatt gattgcggcc agaaagtcag aagccttagc
      361 aaattattgg tattacaaca gcaatattag gggcgtggta tacgcaggac tgagtcgtga
      421 tattagaaaa gaactggcct atgtgattaa tggcaggttc ctgagaaaag atattaagaa
      481 agataaaatc acggaccggg aaatggaaat tatccgcatg acggcccagg gaatgcaacc
      541 taaatcgatt gccagaattg aaaattgtag tgtgaagaca gtgtataccc atcggcgtaa
      601 tgctgaggcc aagctgtact caaaaatata taagttggtt cagtaaactc caggctagtt
      661 agttttaaaa atgactcact gggacatcac gtcctcaatt caactcggga agaaatgcaa
      721 tgaaaaaaaa ggttctggca atagctctgg taacggtgtt taccggtata ggtgtggcgc
      781 aggctgctga cgtaacagct caggctgtag cgacctggtc agcaacagcc aaaaaagaca
      841 ccaccagtaa gctggttgtg acgccactcg gtagcctagc gttccagtat gccgaaggca
      901 ttaaaggttt taactcacag aaaggtctat ttgacgtggc tatcgagggt gactcaacgg
      961 ctaccgcctt taaactgacc tcacgtctta tcaccaacac attaacccag ttggatacct
     1021 caggttccac actgaatgtg ggcgtggatt ataacggcgc ggcagtcgaa aaaactggcg
     1081 ataccgtgat gatcgatacc gccaacggcg tactgggcgg caaccttagc ccgctggcaa
     1141 acggttacaa tgccagcaat cgtaccaccg cacaggatgg tttcactttc tccatcatca
     1201 gcggcaccac caatggtacc accgcagtaa ccgattacag cactctaccg gaaggcatct
     1261 ggagcggcga cgttagcgta cagttcgacg cgacctggac cagttaatct ctctgatgta
     1321 ccagcagggg tagcccccct gctttattcc tggacggaat tgttatgaaa aagcaccttc
     1381 tgcctctcgc tctgctgttt tccggaatat ctccggccca ggcgctggat gtcggcgata
     1441 tatcatcgtt tatgaacagt gacagcagca cgctgagcaa aacgatcaaa aacagtaccg
     1501 acagtggtcg ccttatcaat atccgtctcg aacggctctc ttcaccgctt gacgacgggc
     1561 aggttatctc aatggacaag ccggatgagt tgctactcac tcccgccagc ttgctgctac
     1621 ccgcccaagc cagcgaagtg atccgcttct tctataaggg acccgcagat gaaaaagagc
     1681 gctactaccg cattgtctgg tttgatcagg ccctcagtga tgcgcagcgc gataatgcca
     1741 accgcagcgc tgtggccact gcttccgccc gcatcggcac cattctggtc gtcgcccctc
     1801 gtcaggcgaa ctaccacttt cagtacgcca acggctccct gacaaataca ggaaatgcga
     1861 cgctgcggat cctcgcttac ggcccctgcc tgaaagccgc caatggtaag gagtgtaaag
     1921 agaattacta cctgatgcca ggcaagtcgc gtcgttttac ccgcgtggac acggcggata
     1981 acaaaggacg ggttgcactt tggcagggtg ataagttcat tcccgtgaaa tagatagctg
     2041 tgcagatgga taacgacaat gcctttacga cggttctccc caggactgaa agcccagttt
     2101 gccttcggca tggtcttttt gttcgttcaa cccgatgcca gcgctgctga cataagtgcg
     2161 cagcaaatag gtggggtgat tattccgcag gccttcagtc aggcgcttca ggacggcatg
     2221 agcgtcccgc tctatattca tctcgccggt agccagggtc gccaggacga tcagcgaatc
     2281 ggcagcgctt ttatctggct ggatgatgga cagctacgca tccggaaaat acagctggaa
     2341 gagagtgaag ataacgccag tgtcagcgaa caaactcgac agcagctgat ggctctggcg
     2401 aacgccccgt tcaatgaggc ccttaccatc cccctgactg acaacgcgca gctggatctc
     2461 agcttgcgcc aactgctgct gcagctggtg gtcaagcgcg aagcgctggg caccgtacta
     2521 cgctcacgta gcgaagacat cgggcagtcc agtgttaaca ccctcagcag taatctgagc
     2581 tataacttgg gcgtctataa caaccagttg cgtaacggcg ggagcaacac atccagctat
     2641 ctgtcgctga ataacgttac tgcgctgcgc gaacatcatg tggtgctcga cggctcgctg
     2701 tacgggatcg gtagcggtca acaggacagt gaattatata aagcgatgta tgaacgcgat
     2761 tttgccggtc accgatttgc cggtggaatg ctcgacacct ggaacttgca gtccttaggg
     2821 ccgatgaccg ccatttcagc agggaagatt tacggccttt cctggggaaa ccaggccagc
     2881 tccaccatct tcgacagcag ccagtcagcc acgccagtga tcgccttttt accggcggcg
     2941 ggcgaagtac atctcacccg tgatgggcgg ctactaagcg ttcagaactt cactatgggc
     3001 aatcatgaag tggatacccg gggtctaccg tacgggattt acgatgtgga agttgaggtg
     3061 atcgttaacg gtcgcgtgat cagcaaacgc ccccagcggg tcaataagct gtttagccgg
     3121 gggcgcggcg tcggtgcacc actggcgtgg caggtatggg gcggtagctt tcatatggat
     3181 cgctggtcgg aaaacgggaa aaagacgcga ccagctaaag agagttggct agcaggtgcc
     3241 tcgacctccg gctcattgag tacgcttagc tgggcggcaa cgggatatgg atacgataat
     3301 caggcggtgg gtgaaacccg tctgacgctg ccgcttgggg gggcgatcaa cgttaacctg
     3361 caaaatatgc tggccagtga cagctcatgg agcagcatcg gcagcatcag cgccactcta
     3421 ccgggaggct ttagttcgct gtgggttaac caggaaaaaa cccgcattgg caatcaattg
     3481 cgacgtagcg atgccgacaa ccgtgcaatc ggcggcacac tcaacctgaa ctcactgtgg
     3541 tcgaagctgg gcacattcag catcagctac aatgatgacc gccgttacaa cagccattat
     3601 tacacggcag attactatca aaatgtctac agcggtacct ttggttcgct tggcctgcgg
     3661 gccggtattc agcgctataa caacggcgac agcaacgcca atacagggaa atatatcgct
     3721 ctcgatctct cgctaccact gggcaactgg tttagcgcag ggatgactca tcaaaacggc
     3781 tacaccatgg caaacctgtc agcacgcaaa cagtttgatg aaggaaccat tcgcactgtt
     3841 ggtgccaatc tgtcacgtgc catctccggc gataccggtg atgacaaaac cctcagcggt
     3901 ggggcgtatg cacagttcga cgctcgttac gccagcggaa cgctgaacgt caatagcgcg
     3961 gcggacggct acatcaatac caacttgacc gccaatggca gcgtcggctg gcagggtaaa
     4021 aacatcgctg ccagcgggcg gactgatggc aacgctgggg tgatattcaa caccgggctg
     4081 gaggacgacg gtcagatcag cgccaaaatc aacgggcgga ttttcccgct taacggcaag
     4141 cgtaactatc tcccgctctc tccctatgga agatatgagg tggagttaca gaacagcaaa
     4201 aactcactcg acagctacga tatcgtcagc ggccgcaaaa gccatctgac tctctatcca
     4261 ggcaatgtcg ctgtcattga gccagaggtg aagcagatgg ttaccgtctc cggtcgtatc
     4321 cgtgcggaag acggcacact gctggctaac gcacggatta acaaccatat cggccgaacc
     4381 cgaaccgatg aaaacggcga gtttgtcatg gacgtggata agaaatatcc cactatcgat
     4441 tttcgctaca gtggcaataa aacctgcgaa gtggcactgg aactcaacca ggcgcgcggt
     4501 gccgtctggg tcggtgatgt ggtctgcagc ggcctctcat cgtgggcgga ggtgacgcag
     4561 acaggagaag agaatgagag ttaacctact aataacgatg ataattttcg cgctaatctg
     4621 gccagtaact gagctcagag cggcagtgag caaaacaacc tgggcggatg caccggcacg
     4681 cgagtttgtg tttgtcgaaa acaactcaga cgacaacttt ttcgtcactc ctggcggggc
     4741 gctggatccg cgcctgaccg gtgccaaccg ctggaccggt ttaaaataca ctggttcagg
     4801 aaccatctat cagcaaagcc tcggctacat tgataacggt tacaacaccg gcctttatac
     4861 caactggaag tttgatatgt ggctggaaaa ttcaccagtt tcatctcctt taactggctt
     4921 gcgctgcatc aactggtacg ctgggtgtaa tatgaccacc agtcttatcc tgccgcaaac
     4981 caccgacgcc agtggatttt atggcgcgac ggtgaccagc ggcggcgcga agtggatgca
     5041 cggcatgttg tcagacgcgt tttaccagta tctgcaacaa atgcccgtcg gcagcagctt
     5101 tacaatgacc atcaatgcct gccagacctc tgtgaactat gacgccagca gcggcgcacg
     5161 ctgtaaggat caggcctccg gcaactggta tgttcgcaac gtcacccata cgaaagcagc
     5221 aaatctgcgg ttaataaata cccactcgct ggcggaggta tttatcaaca gcgacggagt
     5281 accgactctg ggcgaaggga acgccgactg ccggacacag accatcggca gcctttcagg
     5341 attaagttgt aagatggtca actataccct gcaaacaaac ggactcagca acacctcaat
     5401 ccatatattc ccggcgatcg ccaactcgtc gttagcctcg gccgtcgggg cgtacgatat
     5461 gcagttcagt ctgaatggca gttcatggaa accggtgagc aataccgcct attactacac
     5521 cttcaacgag atgaagagcg cagactcgat ctatgttttc ttctcgagca acttctttaa
     5581 gcagatggtg aacctcggga tcagcgatat caacaccaaa gatctattca actttcgctt
     5641 tcagaacacc acatcaccgg agtctggctg gtatgaattt tctacctcca acacgctgat
     5701 tatcaaaccc cgtgatttca gcatcagtat tatctccgat gaatatactc agacaccgtc
     5761 gcgggaggga tatgttggca gcggcgagtc ggcactcgat ttcggctata tcgtaaccac
     5821 cagcggtaaa acagctgccg acgaagtgct gatcaaggtg accggacccg cgcaggtgat
     5881 tggcgggcgc tcctattgtg tcttcagctc cgatgacggt aaggcgaaag taccgttccc
     5941 ggcgacactt tcctttatta cccgcaacgg agctacaaaa acctacgatg ccgggtgcga
     6001 tgatagctgg cgggatatga ccgatgcgct gtggttgacc acaccgtgga ctgatatctc
     6061 tggcgaagtg gggcagatgg ataagaccac agtcaaattt tcgattccaa tggataacgc
     6121 catttcgctg cgtacggtag atgataacgg ctggtttggc gaagtcagcg cttcaggaga
     6181 gattcatgtt caggcgacgt ggcgtaacat taactaaggc cctgctgaca gcggtctgta
     6241 tgctggcggc acctttgaca caggcgattt cggtcggcaa tctgacattt tcgctgccgt
     6301 ccgagactga ctttgtcagc aaacgtgtag tgaataacaa caaaagcgca cggatatacc
     6361 gtattgccat cagtgctatt gatagcccgg gcagcagtga attgcgcacc cgaccggtgg
     6421 atggtgaact gctttttgcc ccccgccaac tggcgttgca ggctggtgag agcgagtatt
     6481 ttaaatttta ctatcatggt ccacgggata accgcgagcg ctactaccgg gtctcatttc
     6541 gcgaagtccc cactcgtaac cagacaagac gtagcccaac cggcggcgag gtcagcacgg
     6601 agccggtggt ggtgatggat accattctgg tagtacgacc acgtcaggtt cagtttaaat
     6661 ggtccttcga caaggtgaca ggaacggtaa gtaacactgg caacacatgg tttaagctac
     6721 tgattaagcc aggatgtgat tcgaccgaag aggaaggcga tgcctggtat ctacgtccgg
     6781 gagacgtggt tcatcagcct gagttacgtc agccggggaa tcattatctg gtctataacg
     6841 acaaattcat taagattagc gattcctgtc cggctaagcc cccttcggcg gactaagact
     6901 tatccatcgg tcgagggaag aattccacct cagagctcca aaaatcgttt aaatgatgtg
     6961 gaagcgatcg tcaatgtcac ctgtaacgat gggctttctc atatgcacac catagtgttc
     7021 aggagacacg gaaaggtatc aggcagcctg ctaatgagtg gtttaatcac gaattagtac
     7081 gtaaaatcgg taacggctgg aaatcattca atactcacac tatcgaaagt tccccagcca
     7141 accgcggtac gttcttacat acgatgtacc gctgttctct ttacgatttt tagctgtact
     7201 ggtgaattat gagcaatctg aatccatgca tgacgtgtgg tgcctgttgt gcatttttcc
     7261 gcgtctcttt ttactgggcc gaagccgacg atgctggcgg tactattccc gccaggctca
     7321 ctgaacaaat atcccctttt caccgatgca tgagcggtac caatcagaaa aacccccgat
     7381 gtattgccct tgcaggaacc ccgggcaaaa atgcctgctg cacgatatat aaaaatcgat
     7441 cgtccacatg cagagaattt gccatgtctg gtgagaacgg agtcgtcaat gaggcttgca
     7501 atcgtgcaag ggctaaatac gggctgacac cgttataaac atacaataat taattgcact
     7561 gccccgccag cgataatagc ggggcttcgt ttttcagggg ta
//
LOCUS       DAZFJF010000184         7560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFJF010000184 DAZFJF010000000
VERSION     DAZFJF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.186"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            22..1707
                     /gene="yddA"
                     /locus_tag="ACHKAU_004546"
     CDS             22..1707
                     /gene="yddA"
                     /locus_tag="ACHKAU_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO5533473.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
     gene            1745..4117
                     /gene="yddB"
                     /locus_tag="ACHKAU_004547"
     CDS             1745..4117
                     /gene="yddB"
                     /locus_tag="ACHKAU_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO5533474.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            4174..6957
                     /gene="pqqL"
                     /locus_tag="ACHKAU_004548"
     CDS             4174..6957
                     /gene="pqqL"
                     /locus_tag="ACHKAU_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5533475.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            7319..>7560
                     /locus_tag="ACHKAU_004549"
     CDS             7319..>7560
                     /locus_tag="ACHKAU_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5533476.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         2195 a         1674 c         1703 g         1988 t
ORIGIN      
        1 tggcacaatc aattttcgct aatgataacc attcccatta cgttttgtat gttgatagca
       61 aagtatctct gtctgctgaa gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg
      121 ttaatcatta ttatcctcgc catgatcctt ggcgtggtta aaattcaggt ctggttaaac
      181 gactggaata atgatttttt caatgcgcta agccagaaag aaaccgataa gctttggcag
      241 cttgtccttt ggttccctgc cctgctgggg atctttgtat taatatccgt aaacaaaaca
      301 tggttaatta aactactgac tattcgctgg cgcgaatggt taacagatta ctaccttaat
      361 cggtggtttg cagataaaaa ctactacttc acgcagatct acggcgagca taaaaataca
      421 gataaccctg accaacgtat cgctgaagac attctcttac tgatcagcaa aacgctcagc
      481 ctttcatttg gcttcatcca gtcacttagc atgctgatca cctttaccgt tattctttgg
      541 caaagcgcgg gtacgctctc attcactgtt ggtggaacag aatggaatat ccaggggtat
      601 atggtctata ccgttgtgct cattgtgatc ggtggaactt tatttaccca taaggttggt
      661 aaacgtattc gtccgcttaa tgtggaaaaa caacgtagcg aagcgacatt tcgaacaaat
      721 cttgtgcagc ataataagca ggcagaactg attgcgctaa gcaatgctga gtctctacaa
      781 cgccaggagt tgagcgacaa ttttcatacc attaaagaga actggcatcg tttaatgaat
      841 cgtcagcggt ggcttgatta ctggcagaat atttattcgc gctcgcttag cgttcttccc
      901 tactttctgt tattgccgca gtttattagc gggcagatca atctgggcgg actgatgaaa
      961 tcgcgccagg catttatgct ggtatcgaac aatttaagct ggtttattta taaatatgac
     1021 gaacttgctg aactggctgc ggttatcgat cgtttgtatg agttccatca actcaccgaa
     1081 cagcgcccta cgaataaacc taaaaattgc caacatgcgg tacaagtggc tgacgcgagt
     1141 attcgtactc ctgataataa gatcatatta gagaacctga actttcatgt ttcgccaggc
     1201 aaatggctat tactgaaagg ttactctggt gcgggaaaaa ccacactgct taaaacatta
     1261 tctcactgct ggccgtggtt taaaggtgat atttcttctc ctgctgacag ttggtatgtg
     1321 tcacaaacac cgttaatcaa aaccggctta ctgaaagaga ttatttgtaa agcacttccc
     1381 ctgcccgtag acgataaatc gttgagcgaa gtactgcatc aggttggtct ggggaaattg
     1441 gctgcgcgta ttcatgacca cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa
     1501 cgtatcgccc tggcacgatt aattttacga cgtccgaaat ggatatttct tgacgaaact
     1561 acctctcatc ttgaggaaca agaggctatc cgcttactgc gtttagtgcg tgaaaaacta
     1621 cccacaagcg gcgtcattat ggttacacat caatccggtg tctggaacct ggcagatgat
     1681 atttgtgaca ttagcgcggt tttataaaaa caataaaagc cctgctgtac ggagatataa
     1741 ataaatgaag cgagttctta ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc
     1801 cgtcgatgac aaaaacatgt acatgcatgt ttttgaagag atgacggtct atgctcctgt
     1861 ccctgtaccc gtaaacggca acacgcatta caccagtgaa agcatcgagc gtttaccgac
     1921 cgggaatggc aatatcagcg atctgctgag aaccaaccct gcggtacgca tggattcaac
     1981 gcaaagtacc tcgttgaacc agggagatat tcgcccggag aaaatctcta ttcacggtgc
     2041 gtcgccctat cagaatgcct atttgattga cggtattagt gccactaata acttgaaccc
     2101 agcgaatgag tccgatgcca gtagtgcaac caatattagc gggatgtcac aggggtatta
     2161 tcttgatgtc agcttactgg acaatgtgac gctttatgac agtgttgtgc cggttgaatt
     2221 tggtcgcttc aatggcgggg taattgatgc aaagatcaaa cgcttcaacg ctgatgatag
     2281 caaggtgaaa ttgggttatc gcactacgcg ttcggactgg ttaacatcgc atatcgatga
     2341 gaataacaag agcgcattta atcaaggctc ttcaggaagt acttattact ccccagattt
     2401 taaaaagaac ttttatacct tgtcgtttaa tcaggaactc gctgataact tcggcgttac
     2461 cgccggttta tcgcgccgcc agtctgatat cacccgcgcg gattatgttt cgaatgacgg
     2521 cattgtcgcc ggtcgggcac agtataaaaa cgttatcgat actgcattga gcaaatttac
     2581 ctggtttgcc agcgaccgct ttacccacga tttaacctta aaatataccg gctccagccg
     2641 tgattataat accagcacct tcccgcagtc tgatcgcgaa atgggtaata aatcctacgg
     2701 tctggcatgg gatatggata cgcagctcgc atgggccaaa ctgcgtacca ccgttggttg
     2761 ggatcatatt agtgattata cccgtcacga tcatgacatc tggtacaccg aactttcatg
     2821 tacatatggt gatattacag ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc
     2881 tgtagataat tacaccttca aaacacgcct ggactggcaa aaattcgccg tgggtgatgt
     2941 ttcgcatcaa ccctacttcg gcgcggaata catctattcc gatgcatgga ctgaacgcca
     3001 taaccagtct gaatcctatg tgattaatgc tgcaggaaag aaaactaacc ataccattta
     3061 ccataaaggt aaaggcagcc tgggaattga caactacacg ctgtatatgg cggatcacat
     3121 tagctggcgg aatgtgtcgt taatgcccgg tgtgcgttat gactatgaca actatctgtc
     3181 aaaccacaat atctccccgc gctttatgac ggaatgggat atttttgctg atcaaacctc
     3241 aatgattaca gcaggttata accgttacta tggcgggaat attcttgata tgggattacg
     3301 tgatatccgc aatagctgga cggaatcggt atcaggcaat aaaaccctga cgcgttatca
     3361 ggatttgaaa acgccttata acgatgaact ggcaatggga ttgcagcaga aaatcggtaa
     3421 gaacgttatt gcgcgcgcaa actatgttta ccgtgaagcg catgatcaaa tcagcaaaag
     3481 cagtcgtacc gacagcgcga ctaaaaccac cattactgaa tataacaacg acggcaaaac
     3541 aaaaacgcat tcgttcaacc tcagttttga actggccgaa cccctgcata tcagccaggt
     3601 agatattaac ccgcaaattg tctttagcta tatcaagagc aagggcaact tgtcgttaaa
     3661 caatggttat gaggagagca ataccggtga taaccaggtg gtttataacg gtaatctggt
     3721 ttcttacgat agcgttccag tggcagattt taataaccca ttaaagatct ccttaaacat
     3781 ggatttcacg catcaaccga gcgggttagt gtgggcgaat acgctggcct ggcaagaagc
     3841 gcgtaaagct cgcattatcc tgggtaagac aaatgcgcaa tacatcagcg aatattcaga
     3901 ttacaagcag tatgttgacg aaaaactgga tagcagcctg acctgggaca cccgcttgtc
     3961 ctggacgcca caatttctga aacaacaaaa cctgacgatc agtgccgata ttctcaatgt
     4021 attggatagc aaaaccgctg ttgatacaac gaataccggt gtggcgacct acgccagtgg
     4081 ccgtactttc tggcttgatg tcagcatgaa attttaatag ttcggatttt acttaaccgg
     4141 gtaaattcgc ccggttttcg catggagata attatgagaa acctctgttt cttactgacg
     4201 ttagtggcaa ctctgttgct ccccgggcgg ctgattgccg ccgccttacc gcaggatgaa
     4261 aagttaatta ccgggcaact gggcaatggc ttgcgatata tgatttatcc gcatgctcat
     4321 ccaaaagatc aggtaaattt atggctgcaa attcataccg gttcattgca ggaagaagac
     4381 aatgagcgcg gcgtggctca ttttgtagaa catatgatgt ttaacggcac aaaaacatgg
     4441 ccgggtaata aagtcatcga aacatttgag tcaatgggcc tgcgttttgg tcgcgatgtt
     4501 aatgcctata ccagctatga cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa
     4561 caaaatctgc aacaagtgat ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa
     4621 aaactcgaag tagacgctga acgtggcgta attactgagg aatggcgtgc ccatcaggat
     4681 gcgaaatggc gcacctctca ggcgcgccgc cctttcctgc tggctaatac ccgtaattta
     4741 gaccgtgaac ctatcggcct gatggatact gtcgccacgg tcacaccggc acaattgcgc
     4801 caattttatc aacgctggta tcaaccaaat aatatgacct ttatcgtggt tggcgatatc
     4861 gacagtaaag aagcgctggc gctgataaag gataatttaa gtaagcttcc ggctaacaaa
     4921 gcagctgaaa atcgcgtctg gccgacaaaa gccgaaaacc acctgcgctt taatatcatt
     4981 aatgataaag aaaaccgggt gaacggcatc gcactctatt atcgcctgcc aatggtacaa
     5041 gtgcacgatg agcaaagctt tatcgaacaa gctgaatgga gcatgttagt tcagctgttc
     5101 aatcaacgtc tgcaggaacg catacagtcg ggcgagttga agactatttc tggcggcact
     5161 gcgcgcagcg ttaaaattgc acccgattat cagtcgctgt ttttccgtgt aaatgcacga
     5221 gacgataata tgcaggatgc tgcgaatgca ttaatggcag agttggcaac tattgatcag
     5281 catggttttt ctgctgaaga actcgatgat gttaaatcta cccgcctcac ctggctgaaa
     5341 aatgcggttg atcagcaagc tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc
     5401 agctcattaa ataatacgcc gttcttgtcg ccggaagaga catatcaact ttcgaaacgt
     5461 ctgtggcagc aaattaccgt gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac
     5521 caggacgcat tttgggagca aatggtaaac aatgagcttg ccgccaaaaa agcattgtct
     5581 cctgcggcta tcctggcgct ggaaaaagag tacgccaaca aaaagctggc ggcttacatc
     5641 ttcccaggca gaaatttatc gttaacagta gacgctgacc cacaggcgga aattagcagc
     5701 aaagaaacgc tggctgagaa tctgacatca ttaacacttt ccaatggtgc cagggttatt
     5761 ctggcaaaat ccgcgggtga agagcaaaag ctacaaatta ctgccgtatc gaataaaggc
     5821 gatttaagtt tccctgcgca gcaaaaatca cttatcgcgc tggcaaataa agcagtaagc
     5881 ggaagcggcg ttggtgagct ctcctcttcc agcctgaaac gctggagtgc ggaaaattcg
     5941 gtaaccatga gcagtaaagt cagtggcatg aatacgttgc tctccgttag cgcgcggact
     6001 aataaccctg aacctggttt tcagttgatt aaccagcgaa tcacccacag cacgattaac
     6061 gacaatattt gggcatcgct acaaaatgct caaattcagg cgttgaaaac gctcgaccag
     6121 cgtccagcgg agaaattcgc ccagcagatg tatgagacgc gctatgctga tgaccgcacg
     6181 aaattactgc aagaaaatca gattgtacag tttactgccg cagatgcgct ggctgccgat
     6241 cgccagttgt tttcatctcc agcggatatc acgtttgtca ttgtcggtaa tgtctcagaa
     6301 gacaaactcg tggcgttaat tacgcgttac ttaggatcaa tcaaacactc tgattcgcca
     6361 ttagccgcag gtaaaccatt aactcgcgcg acggacaacg catcggttac tgtaaaagaa
     6421 caaaatgaac ctgtggcaca ggtttcacag tggaagcgtt atgattcccg gacacctgtt
     6481 aatctggcga cgcgtatggc gctcgatgct tttaacgtcg cactggcaaa agatctacgt
     6541 gttaatattc gtgaacaggc atctggagca tacagcgttt cttctcgcct ctcggttgat
     6601 cctcaggcca aagatatcag tcatttgctg gcttttactt gtcaaccaga acgacatgat
     6661 gaactgttaa cgttagcgaa tgaagtgatg gttaagcgtc tggctaaagg gatcagtgag
     6721 caagaactga atgaatacca gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc
     6781 gttcaacaat tagcgaacac tattgtaaat agtcttattc aatatgacga tcctgcagca
     6841 tggactgagc aggagcaatt gttgaaacaa atgacggtag agaatgttaa cactgccgtt
     6901 aaacaatatc tttctcatcc ggtaaatact tataccggag tattattgcc aaaataataa
     6961 cagccccgtc aacacatcgt tggcggggat tttagcaata ttcgctattt ttatgtaata
     7021 attttataaa tgcgttcaaa ataataatca agtactaata gtgatatttt aaggtctgat
     7081 ttttacgtga taattcagga gacacagaat gcgcataaaa ataacagcat aaaacacctt
     7141 accaccaccc aagaatttca tattgcattg tttttcaatg aaaaaatatt attcgcgtaa
     7201 tatctcacga taaataacat taggattttg ttatttaaac acgagtcctt tgcacttgct
     7261 tactttatcg ataaatccta cttttttaat gcgatccaat cattttaagg agtttaaaat
     7321 ggataagaag caagtaacgg atttaaggtc ggaactactc gattcacgtt ttggtgcgaa
     7381 gtctatttcc actatcgcag aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt
     7441 cgcattccag attatcaatg acgaattata tcttgatggc aacgctcgtc agaacctggc
     7501 cactttctgc cagacctggg acgacgaaaa tgtccacaaa ttgatggatt tatccattaa
//
LOCUS       DAZFJF010000185         7462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFJF010000185 DAZFJF010000000
VERSION     DAZFJF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.187"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..843)
                     /locus_tag="ACHKAU_004550"
                     /pseudo
     CDS             complement(20..843)
                     /locus_tag="ACHKAU_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(897..2615)
                     /gene="proS"
                     /locus_tag="ACHKAU_004551"
     CDS             complement(897..2615)
                     /gene="proS"
                     /locus_tag="ACHKAU_004551"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO5533477.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2727..3434)
                     /gene="tsaA"
                     /locus_tag="ACHKAU_004552"
     CDS             complement(2727..3434)
                     /gene="tsaA"
                     /locus_tag="ACHKAU_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO5533478.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3431..3835)
                     /gene="rcsF"
                     /locus_tag="ACHKAU_004553"
     CDS             complement(3431..3835)
                     /gene="rcsF"
                     /locus_tag="ACHKAU_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO5533479.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3953..4768)
                     /gene="metQ"
                     /locus_tag="ACHKAU_004554"
     CDS             complement(3953..4768)
                     /gene="metQ"
                     /locus_tag="ACHKAU_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO5533480.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4808..5461)
                     /locus_tag="ACHKAU_004555"
     CDS             complement(4808..5461)
                     /locus_tag="ACHKAU_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO5533481.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5454..6485)
                     /gene="metN"
                     /locus_tag="ACHKAU_004556"
     CDS             complement(5454..6485)
                     /gene="metN"
                     /locus_tag="ACHKAU_004556"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO5533482.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6673..7248
                     /gene="gmhB"
                     /locus_tag="ACHKAU_004557"
     CDS             6673..7248
                     /gene="gmhB"
                     /locus_tag="ACHKAU_004557"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO5533483.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1835 a         1935 c         1897 g         1795 t
ORIGIN      
        1 gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc ttgatataaa
       61 tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac gcagagacat
      121 caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc gccagtgtaa
      181 gtggatgccc ggctttggca aaggcatccg tgacgaattc cgaacaaaac cacgactttt
      241 tgtctccttc gcccacactg cttaactgcg ctttcgccag gccgctgacg cactttggcg
      301 aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa agggaataaa
      361 ttcgacaatg ccgcgatagt tataaccgct atctttgatt ttattggcaa acgcggtgat
      421 atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcactatg
      481 cttcatcgct tttttaaggg aaacaatctg gacgccagcg cctgtcgctt ctgcaacgtt
      541 attatcaccc agaaagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
      601 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtgc
      661 cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt tgacagccca
      721 tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga tatcaacggt
      781 acaagccgaa agtaaaagaa agctggggag aaacaggcgg cagtacgcct ttggtttatc
      841 cattttatac aatccatgta aaaaaagggc cctgaaattc aggacccttt ctggcatcag
      901 cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa ctgtttctcg
      961 ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc gcccagcaca
     1021 atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg acgctctttg
     1081 cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag tttctcagca
     1141 agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac ctggaacggc
     1201 gcgatagcgt caggccatac gatgcctcgt tcgtcgtagt tctgctcaat cgccgcagct
     1261 accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt acggccatct
     1321 tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg gaagatgtga
     1381 ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct tggatcgcca
     1441 gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc ccagttgatg
     1501 ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat cgccgcaacg
     1561 gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc cggaccggct
     1621 ttaaccacgg cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc aacctgcggc
     1681 agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag cgcaaccagc
     1741 gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt ctcaatcggc
     1801 agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc aaccagcgtc
     1861 atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc cagttcaatg
     1921 ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct ctgcgccagc
     1981 acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcttg tacggcgcgg
     2041 aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc atcgtaggtt
     2101 tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag gaattcgcgg
     2161 gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg gatctgatag
     2221 aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc agtgataact
     2281 tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa acgcagcagt
     2341 tccggaccgt actgttccca acgaccactc tcttgccaca aatcagctgg ctgaaccacc
     2401 ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat gttttcgact
     2461 tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc cagcttgcgg
     2521 atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc aggtgtctcc
     2581 ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca gttggaaggt
     2641 agaacaggct caaggcgagc ctgggacaaa aaaaagtgat ttagtttacc agtgcaaaag
     2701 aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac ttcaaaacct
     2761 gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata ggttttgccc
     2821 gtttcctcac ctttacgata ggccgggcgt gggtcctgcg ccagtacttc gcggataaac
     2881 agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac ctctgcggta
     2941 aaactcaccg ccatctctgc cgctggcgca ctttgcgcat agctggcact ggcatcggga
     3001 agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc atcgaccaga
     3061 tccagactgc cgagcttcag aatcacgctg tctttatggc aaacaacctc tttcagctct
     3121 accagcgaca taccaattgg gttagggcgg aaagtagagc gtgttgcgaa aacccccatt
     3181 ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc ttccatcgtt
     3241 tgatgaaaga cgaaaaggat ccataaatgg ctgaacgctt ccaggccgcg aacggcgtcg
     3301 gcctggttgt agggagcaat gagatgcagt tcgccgttgg cgctttttac cagacctggc
     3361 tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat ttgctcgaac
     3421 tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca gcctgacgat
     3481 agcagcctgg cgtaccgctg gtgacttcgc agctatgcag taataccgca ttggccttca
     3541 ttttcgaggc gttgatttgc atccgcttac gcgcggttgg aatgctcggc ggagagtcct
     3601 gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac ggtttgccga
     3661 ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct ttcggttttg
     3721 caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg gatctgctta
     3781 acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca cgcatagtat
     3841 ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa atgacaagac
     3901 gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa aattaccagc
     3961 ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca tcagactgat
     4021 aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc gttacgatca
     4081 ggtttacgta cggggagtct ttatcttcga caaagatacc gtctttcgct ggagtcaggc
     4141 caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg tcgtccagag
     4201 atcgaggcag ctgcggtgct tccagctcaa caattttcag attttttggg ttctcaacaa
     4261 catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag cccacttttt
     4321 gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca acctgcgaac
     4381 catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc ggataaacaa
     4441 aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga tcaaggtacg
     4501 gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg tttggcagaa
     4561 catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc gcaactttct
     4621 gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta atgtggtttg
     4681 gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg gctcccactg
     4741 ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat ttatgttgtg
     4801 ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac tgaattaaat
     4861 aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgtta tagccgatgt
     4921 agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca cccatcgcgg
     4981 aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc ggcaatgctt
     5041 ccggtaacag cactttacgg acgatctgca tcggcgtggc acccattgca cgggaagctt
     5101 caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca ataaacggtg
     5161 ctgcaccaac ggttaacgga acaatcgctg cctgcaaacc aatcgatgta ccgacaataa
     5221 cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa cggaaaatgt
     5281 tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc cccgggcgcg
     5341 taacataaag caggacgccg accggcagac caatcacaaa gccaaaaaat ccggatacga
     5401 aggtcattgc cagcgtttcc catacgccac gaaccagcaa ccacatcatc ggctcagaca
     5461 taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc ggcttgcgta
     5521 tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga acttaacgcc accggcgtaa
     5581 tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt ttcagaaagc
     5641 agcggggcat cgaccgattg accggtaaac tccaggcgca acatcggcac gcagtcagta
     5701 aatggctccg cttgcagacg ttcctggtaa tcttccggga tatccagatg cagggtcgac
     5761 tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact taccgtgtcc
     5821 tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg cttcacaacg
     5881 tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat gtctttcagc
     5941 agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc atcacacagc
     6001 aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt ctgcccaccg
     6061 gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga cagcaattcc
     6121 gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg cagagccacg
     6181 ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat accaatctgg
     6241 cggcgagctt tggtcaactc ggattctgac agcgtggtca gttcctggcc atcgaccagc
     6301 acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt actcttaccc
     6361 gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag gctgacgttg
     6421 ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt cgaaagtttt
     6481 atcattgatt atttattatc gtcattaagt tagtcgtggc atctcgaatg cctgaaacgg
     6541 gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa tcaggtttat
     6601 gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg ccttattaag
     6661 gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga tggcaccatt
     6721 aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga cggtgttatt
     6781 gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac caaccagtct
     6841 ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga gtggatggac
     6901 tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc gcatcatccg
     6961 cagggtagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca tccggggatg
     7021 cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat ggtgggcgat
     7081 aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt gctggtgcgt
     7141 acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt aaatagcctg
     7201 gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cacaatgatt aaaagatgag
     7261 cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc tataatgcgc
     7321 ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg ttgaagagaa
     7381 aaaatcctga aattcagggt tgactctgaa agaggaaagc gtaatatacg ccacctcgcg
     7441 acagtgcgct aaagcgcgtc gc
//
LOCUS       DAZFJF010000186         7325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFJF010000186 DAZFJF010000000
VERSION     DAZFJF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.188"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..865
                     /locus_tag="ACHKAU_004558"
     CDS             <1..865
                     /locus_tag="ACHKAU_004558"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5533484.1"
                     /translation="IKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHS
                     NYGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQYRMDATRQMFAQKVSAYT
                     GLSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKE
                     TQSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEA
                     HGREEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPAS
                     DAVNDLLNTPV"
     gene            875..1207
                     /locus_tag="ACHKAU_004559"
     CDS             875..1207
                     /locus_tag="ACHKAU_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5533485.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            1263..2288
                     /locus_tag="ACHKAU_004560"
     CDS             1263..2288
                     /locus_tag="ACHKAU_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5533486.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            2330..2725
                     /locus_tag="ACHKAU_004561"
     CDS             2330..2725
                     /locus_tag="ACHKAU_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5533487.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            2737..3111
                     /locus_tag="ACHKAU_004562"
     CDS             2737..3111
                     /locus_tag="ACHKAU_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5533488.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            3102..3680
                     /locus_tag="ACHKAU_004563"
     CDS             3102..3680
                     /locus_tag="ACHKAU_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5533489.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            3677..4072
                     /locus_tag="ACHKAU_004564"
     CDS             3677..4072
                     /locus_tag="ACHKAU_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5533490.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELYIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            4080..4820
                     /locus_tag="ACHKAU_004565"
     CDS             4080..4820
                     /locus_tag="ACHKAU_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5533491.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            4836..5258
                     /locus_tag="ACHKAU_004566"
     CDS             4836..5258
                     /locus_tag="ACHKAU_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5533492.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            5240..5674
                     /locus_tag="ACHKAU_004567"
     CDS             5240..5674
                     /locus_tag="ACHKAU_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5533493.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            5667..>7325
                     /locus_tag="ACHKAU_004568"
     CDS             5667..>7325
                     /locus_tag="ACHKAU_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5533494.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYKRLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQE"
BASE COUNT         1668 a         1902 c         2307 g         1448 t
ORIGIN      
        1 cataaaaccg gtatgggcgc ttgccaacga catgaactgc agtgcaggtc agttgcttgc
       61 cagtgccgcc tcccggcgtc tggtcacgca gaccgcccgg acaggctcca tcggcgtcat
      121 gatggctcac agtaattacg gtgctgcgct ggagaaacag ggtgtggaaa tcacgctgat
      181 ttacagcggc agccataagg tggatggcaa cccctacagc catcttccgg atgacgtccg
      241 ggagacactg cagtaccgga tggacgcaac ccgccagatg tttgcgcaga aggtgtcggc
      301 atataccggc ctgtccgtgc aggctgtgct ggataccgag gctgcagtgt acagcggtca
      361 ggaggccatt gatgccggac tggctgatga acttgttaac agcaccgatg cgatcaccgt
      421 catgcgtgat gcactggatg cacgtaaatc ccgtctctca ggagggcgaa tgaccaaaga
      481 gactcaatca acaactgttt cagccactgc ttcgcaggct gacgttactg acgtggtgcc
      541 agcgacggag ggcgaaaacg ccagcgcggc gcagccggac gtgaacgcgc agatcaccgc
      601 agcggttgcg gcagaaaaca gccgcattat ggggatcctc aactgtgagg aggctcacgg
      661 acgcgaagaa caggcacgcg tgctggcaga aacccccggt atgaccgtgg aaacggcccg
      721 ccgcattctg gccgcagcac cacagagtgc acaggcgcgc agtgacactg cgctggatcg
      781 tctgatgcag ggtgccccgg caccgctggc tgcaggtaac ccggcatctg atgccgttaa
      841 cgatttgctg aacacaccag tgtaagggat gtttatgacg agcaaagaaa cctttaccca
      901 ttaccagccg ctgggcaaca gtgacccggc tcataccgca accgcgcccg gcggattgag
      961 tgcgaaagcg cctgcaatga ccccgctgat gctggacacc gccacccgta agctggttgc
     1021 gtgggatggc accaccgacg gtgctgccgt tggcattctg gcggttgctg ctgaccagac
     1081 cagcaccacg ctgacgttct acaagtccgg cacgttccgt tatgaggatg tgctctggcc
     1141 ggaggctgcc agcgatgaga cgaaaaaacg gaccgcgttt gccggaacgg caatcagcat
     1201 cgtttaacct tacccttcat cactaaaggc cgcctgtgcg gcttttttta cgggattttt
     1261 ttatgtcgat gtacacaacc gcccagctgc tggcggcaaa tgagcagaaa tttaagtttg
     1321 atccgctgtt tctgcgtctc tttttccgtg agagctatcc cttcaccacg gagaaagtct
     1381 atctctcaca aattccggga ctggtaaaca tggcgctgta cgtttcgccg attgtttccg
     1441 gtgaggttat ccgctcccgt ggcggctcca cctctgaatt tacgccggga tatgtcaaac
     1501 ccaagcatga agtgaatccg cagatgaccc tgcgtcgcct gccggatgaa gatccacaga
     1561 atctggcgga cccggcttac cgccgccgtc gcatcatcct gcagaacatg cgagacgaag
     1621 agctggccat tgctcaggtc gaagagatgc aggcagtttc tgccgtgctt aagggcaaat
     1681 acaccatgac cggtgaagcc ttcgatccgg ttgaagtgga tatgggccgc agtgtggcga
     1741 acaacatcac gcagtccggc ggtacggagt ggagcaagcg tgacaagtcc acgtatgacc
     1801 cgaccgacga tatcgaagcc tacgcgctga acgccagcgg tgtggtgaat atcatcgtgt
     1861 ttgatccgaa aggctgggcg ctgttccgtt ccttcaaagc cgtcaaggag aagctggata
     1921 cccgtcgcgg ctctcattcc gagctggaga cagcggtaaa agacctgggc gaagcggtgt
     1981 cctataaggg gatgtatggc gatacggcga tcgtcgtgta ttccggacag tacgtggaaa
     2041 acgacgtcaa aaagaacttc cttccggaca acacgatggt gctggggaac actcaggcac
     2101 gcggtctgcg cacctatggc tgcattcagg atgcggacgc acagcgcgaa ggtattaacg
     2161 cctctgcccg ctacccgaaa aactgggtga ccaccggcga tccggcgcgt gagttcacca
     2221 tgattcagtc agcaccgctg atgctgctgg ctgaccctga tgcgttcgtg tccgtacaac
     2281 tggcgtaatc atggcccttc ggggccattt tctctctgtg gaggagtcca tgacgaaaga
     2341 tgaactgatt gcccgtcttc aggtgctggg tgagcaactg aaccgtgatg tcagcctgac
     2401 ggggacgaaa gaagaactgg cactccgtgt ggcagagctg gaagaggagc ttgatgacac
     2461 ggatgacgct gccggtcagg acacatctgt cagcccggaa aatgcgctga ccggacatga
     2521 aaatgaggtt gtatcagcgc agccggatac cgtgactgat acggctgatc tggtcacggt
     2581 tgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggatg aggctgtggc
     2641 atttgtgctg ccgggaacgg cgttccgtgt ctctgccggt gtggcagctg aaatgacaga
     2701 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     2761 gatgctgcca ttgcctgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     2821 atgacatccg gtgagcagtc cggtgctgtg atacgtggtg tttttgatga ccctgaaaat
     2881 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     2941 actgatgatg tgcggcagct gcggcgcggc gacacgctga ccatcggtga ggaaaacttc
     3001 tggatagacc ggatttcgcc ggatgatggc ggaagctgtc atctctggct tgggcggggc
     3061 gtaccgcctg ccgttaaccg tcgccgctgg aagggggatg tatggccata aaaggtcttg
     3121 agcaggccgt tgaaaacctc agccgtatca gcagaacggc ggtgcccggt gccgccgcaa
     3181 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc ggtgtcacag gttgcccgtg
     3241 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     3301 aaaatccgca ggccagaatc aaggttaacc ggggggattt gcccgtaatc aagctgggta
     3361 atgcgcgggt tgtcctttcg cgccgcaggc gtcgtaaaaa ggggcagcgt tcatccctga
     3421 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     3481 aactgaaaaa tggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     3541 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa caaaatattg
     3601 agcggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     3661 ggatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     3721 gaagcatgac accggggcga cgttttttga tggtcgcccc gctgtttttg atgaggcgga
     3781 ttttccggca gttgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     3841 cgatacctgg caggcggagc tgtatatcga agttttcctg cctgctcagg tgccggattc
     3901 agagctggat gcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     3961 gtcagagttg atcaccagta tggtggccag tggctatgac taccgacgcg acgatgatgc
     4021 gggcctgtgg agttcagccg atctgactta tgtcattacc tatgaaatgt gaggacgcta
     4081 tgcctgtacc aaatcctgta atgccggtga aaggtgccgg gaccaccctg tgggtttata
     4141 aggggagcgg tgacccttat gcgaatccgc tttcagacgt tgactggtcg cgtctggcaa
     4201 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
     4261 atgatgaaga tgcggactgg actgcgaccg ggcaggggca gaaatcagcc ggagatacca
     4321 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
     4381 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
     4441 gtggctgggt cagcagtatc ggtaaagcgg tgacggcgaa ggaagtgatc acccgcacgg
     4501 tgaaggtcac caatgtggga cgcccgtcga tggcagaaga tcgcagcacg gtcacagcgg
     4561 caaccggcat gaccgtgacg cctgccagca cctcggtggt gaaagggcag agcaccacgc
     4621 tgaccgtggc attccagccg gaaggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
     4681 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
     4741 caggcaaggt caacattccg gtcgtatccg gtaatggtga acttgctgcg gttgcagaaa
     4801 tcactgtcac cgacagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
     4861 aacataacgg cgtgaccgtc acgctttctg aactttcagc cctgcagcgt attgagcatc
     4921 tcgccctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttacag
     4981 tggaagacgc catcagaacc ggtgcttttg tggtggcgat gtccctgtgg cataaccatc
     5041 cgcagaagac aaagcagcct tccatgaatg aagccgttaa acagattgag caggaagtgc
     5101 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
     5161 gtatgtatga gtttgtggtg aatgatgccc ctgaacaggc agaggacgcc gggcctgcag
     5221 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
     5281 cgagatgggg cgacccgact ggcgtgccat gcttgccggg atgtcatcca cggagtatgc
     5341 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
     5401 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggata tgcatccgct
     5461 ggatttcagt ctgctgaacc ggcgtgaggc tgacgaagag cctgaagatg atgtgctgat
     5521 gcagaaagcg gcagggcttg ccggaggcgt ccgctttggc ccggacggga atgaagttat
     5581 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
     5641 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
     5701 taagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
     5761 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
     5821 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
     5881 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg cgggcaaagt ccgtggctga
     5941 tcctgctgca acaggggggg caggtgaagg actccttcgg cgggatgatc cccatgttca
     6001 gggggcttgc cggtgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
     6061 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
     6121 cgctggtcct ttccggcaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
     6181 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
     6241 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
     6301 tctcctctgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
     6361 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
     6421 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
     6481 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
     6541 gcacgctgga gacctgggca gacaggactg cgcgggcctt caaatccatg tgggatgcgg
     6601 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
     6661 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttattttgtt aacgatgaag
     6721 cgcgggcgcg ttactgggat gatcgtgaaa aggcccgtct tgcgcttgaa gccgcccgaa
     6781 agaaggctga gcagcagact caacaggaca aaaatgcgca gcagcagagc gataccgaag
     6841 cgtcacggct gaaatatacc gaagaggcgc agaaggctta caaacggctg cagacgccgc
     6901 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac gggaaaatcc
     6961 tgcaggcgga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
     7021 aaaagccgaa acagtccggc gtgaaggtgt ctgcgggcga tcgtcaggaa gacagtgctc
     7081 atgctgccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
     7141 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
     7201 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
     7261 aagatgagac gctggagtac aaacgccagc tggctgcact tggcgacaag gttacgtatc
     7321 aggag
//
LOCUS       DAZFJF010000187         7317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFJF010000187 DAZFJF010000000
VERSION     DAZFJF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.189"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(118..822)
                     /locus_tag="ACHKAU_004569"
     CDS             complement(118..822)
                     /locus_tag="ACHKAU_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5533495.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(959..1411)
                     /gene="moaE"
                     /locus_tag="ACHKAU_004570"
     CDS             complement(959..1411)
                     /gene="moaE"
                     /locus_tag="ACHKAU_004570"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO5533496.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1413..1658)
                     /gene="moaD"
                     /locus_tag="ACHKAU_004571"
     CDS             complement(1413..1658)
                     /gene="moaD"
                     /locus_tag="ACHKAU_004571"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO5533497.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1651..2136)
                     /gene="moaC"
                     /locus_tag="ACHKAU_004572"
     CDS             complement(1651..2136)
                     /gene="moaC"
                     /locus_tag="ACHKAU_004572"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO5533498.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2139..2651)
                     /gene="moaB"
                     /locus_tag="ACHKAU_004573"
     CDS             complement(2139..2651)
                     /gene="moaB"
                     /locus_tag="ACHKAU_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO5533499.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2673..3662)
                     /gene="moaA"
                     /locus_tag="ACHKAU_004574"
     CDS             complement(2673..3662)
                     /gene="moaA"
                     /locus_tag="ACHKAU_004574"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO5533500.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3650..3794)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4059..4967
                     /gene="yvcK"
                     /locus_tag="ACHKAU_004575"
     CDS             4059..4967
                     /gene="yvcK"
                     /locus_tag="ACHKAU_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO5533501.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5159..7180)
                     /gene="uvrB"
                     /locus_tag="ACHKAU_004576"
     CDS             complement(5159..7180)
                     /gene="uvrB"
                     /locus_tag="ACHKAU_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO5533502.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1754 a         1953 c         1843 g         1767 t
ORIGIN      
        1 acggaaaacg cttccgctcg ccataaggcg tattttatac ttggtacttg ttaagtgtcg
       61 tttttagtaa gaaaaaaaat cccgacacat ggccgggata ttgatggtga aaaagaatta
      121 acggcggttg ccaaaaattc gcagcaacat caggaacagg ttgatgaagt ccagatacaa
      181 ggttaacgcg ccaagaatgg aatatttgcg caggttcgac gtgtcgcggg tatcaatctg
      241 ctcacccata tttttcaatt tctgagtgtc atacgccgtc aatccgacaa agacaatcac
      301 gccgatgtag gtaactgccc acatcaatgc ttcgcttttc agccagaagt tgaccagcga
      361 tgccagcaca atgccgatta acgccataaa cagcatattg ccaaagccac ttaaatcgcg
      421 cttcgtggtg taaccgtaca ggctcattgc gccgaacatc ccggcagtaa cgacgaaagt
      481 actggcgata gaagcagcgg tatagacaat gaatatactg gaaagcgtaa gacccgtcag
      541 cgccgaataa agcataaaga gcatcgtcgt tacacctgcg ctcagctttt gaatcatcgc
      601 tgataacaca ataaccaatg ctaattgcgc gatgatcaga ccgattaaaa agacacggtt
      661 agtaaacaac agctccatca cggccgcgga attagccgca taccaggcaa caaatgcggt
      721 cagcaacaag ccaacggtca tccagccata gacttgcgcc atataagttt gcaagccagc
      781 ccggggttgt acgattgaat cagaacgtgg gaatctgtcc atgacgatct cctgaagata
      841 taaggaatat cttaaggata ctgcaaaatg atgaggctgt gcatcgacgc agcgtaaacg
      901 catgtactga gcggtgaaat tgccggacgc agcggtgcct tatccggcta acaaaaaact
      961 accagcgttt tgccgcctgc tgatcgctct cccgagcttc aacccagcgg tcgccttccg
     1021 gcgtggcttc gcgcttccag aacggtgcgc gggttttgag ataatccata ataaactgcc
     1081 cggcttcaaa cgcactgctg cgatgcgcac tggtgacacc gacaaaaacg atttcatcgc
     1141 ccggccataa ttccccgatg cggtgaatca cagtgacgcg ccccagcggc cagcggttac
     1201 gcgcttcatc aacaatttct gccagtgctt tttcagtcat ccccggatag tgttcgaggg
     1261 ttaatgcttt gacgctgtcg cccaggttat ggttgcgcac cttaccagta aaggtgacta
     1321 ccgcaccgtc ttcgtcacgc tccgccagcc acgggtactc ttctcctacg ctgaacggct
     1381 gcggaccaac aacaattttg gtttctgcca tcttaacctc cggttaccgg cgggaagaaa
     1441 gctacttcgt cgccgtcagt cagcggatgg tcaaaactca ccagcgtctg gttgacggca
     1501 gccagtaatt tgccatcttc cagcgccagc gcccagcgat cgctctgcgc agccatgtgc
     1561 tggcgtaacg cttcaacagt tgggaaatcc gcagccactt cggttgcatc tgttcccacc
     1621 aactcgcgca cctgggcgaa aaaaagaact ttaatcatcc gcttccacct taaagtcacc
     1681 cgacttgccg ccgctcttcg ccagcaaacg taccggacca atcaccatat ctttttgcac
     1741 cgctttgcac atgtcataaa tggtcagcgc cgccacggag gccgcggtta atgcttccat
     1801 ttcgacaccg gttttcccgg tcaggcggca taaggtttct atacgcaccc gattgtgctc
     1861 cggctcggcc tgtaaattga cttcaacttt gctgagcatc agcggatgac agagcgggat
     1921 cagatcccag gtgcgttttg ccgcctgaat accggcaata cgcgcagtgg caaatacgtc
     1981 gcctttgtgg tggcgaccat caataatcat cgccagcgtc tcgctgcgca tggtgacaaa
     2041 ggcttccgcc cgcgcttcac gcacggtttc cgctttggcg gagacatcca ccatgtgcgc
     2101 ttcgccagcg gcgttgatat gggtcagttg cgacatactt atttcttcaa atgtggatgg
     2161 aaattacacg gacgcgtacg ggcatccagc tgcggcgcga tgatattttc ccatgcggta
     2221 cggcacgctt tggtcgaacc cggcatggcg aaaatcagcg ttttgttggc gacgcccgct
     2281 accgcacgag attgcaacgt ggaagtgcca atctcttcaa acgacaacat acggaacact
     2341 tcaccaaaac cttcaatttc acggtcgaac aacggcagca atgcttcggg agcctgatca
     2401 ccttccgtca ggccagtacc acccgtaatc aataccactt gtacatcgtc gctggcgatc
     2461 cacgcagata cctgagcgcg aatagcgtag cggttttctt tcacaatggc tttatcgaca
     2521 acgtgatggc ccgcttcttg cgccgaatcg cgcagatagt gaccggaggt atcgtcttct
     2581 tcaccgcgac gattagaaac cgtaagaata gcaatacggg tcgggataaa ttcagtgctt
     2641 acctgactca tctgatctct ccttttgacg ttttagccgc caatgtacga taagttttgc
     2701 gtaataccgg tgttgttttg atgcaggaaa tgggtctgtt tcttctcccg cagcgccgct
     2761 gaaatacgcg cttccagcgc ctgttgctgg gtatcgtctt ccagcagatc gcgcaggtta
     2821 acgccgcctt caccaaacag gcagagatgg agtttaccaa tggaggaaac gcgcaggcgg
     2881 ttgcaagtgg cgcagaagtc tttttcatac ggcatgataa ggccaatctc tccggcgtaa
     2941 tctggatggc aaaagacttg cgcgggaccg tcgctgcgtt gacgtaattg gtggatccag
     3001 ccgcgacgca gtagctcgtc acgcagaacc tgaccagaga tgtgatgctt acggaagagc
     3061 tcgctgccct cgcccgtttc catcagttcg atgaaacgca gctggatagg gcgatgctgg
     3121 atccagttca gaaaggtgtc gagctggtga tgattaacat cacgcatcag cacggtattg
     3181 actttgacct tctcaaaacc ggcctcaaat gcagcatcaa tccccgccat gacctggttg
     3241 aatttatcct gcccggtaat agcgtgaaac tggcgggcgt ccagactgtc gacactgacg
     3301 ttaatgccag taagtcccgc atcgcgccag ttcgccacat cgcgttccag acggtaacca
     3361 ttggtggtga ccgcaatctg gcggatagcg tcgttttccc gcacagcggc gatgatatcg
     3421 gtaaagtcgc ggcgtaaaga cggctcaccg cctgtcaggc gcactttttc ggtgcccaga
     3481 ctggcgaagg cgcgcgtaac ccggcgaatt tcatcgacgg taagaaagcc tttattggtg
     3541 acgccgctcg gtttgtagcc atccggcagg cagtaggtgc aacgaaagtt acacacatcg
     3601 gtaatcgaca ggcgcaagta gtaaaactta cgcgcaaatg catcagtcag ttgtgaagcc
     3661 atgtacacct ttccagatac gggaggcgaa gtcatttctt ccttcgccct ggtggcaatc
     3721 tttacgagca ttgtcacggc caaagcaccg tatcagttga cccaggtgca gaggctagag
     3781 tgtttagtgg ttatgccgat actagcgtgt aaatgagatt tttaccatcc acactttcgc
     3841 tatgtaatca tgtatatagc gtcatgatcg tgctatttcg ctacagaata agggaaaatg
     3901 gcggttttac tttgatatac atcatcaggg aggcatagcg tgcatcgtcc tttagaggta
     3961 gaagaaaata gtttgtatcc tcaatattgg caggttaatt gctgtttccc cgcaatttgc
     4021 gctactgtag cgcggataat ctgactccag gaaactatat gcgcaatcgt acgctggctg
     4081 atcttgatcg tgtcgttgct ctcggcggag ggcatggact gggacgcgtt ctctcatcac
     4141 tttcgtcttt gggttctcgt ttaacgggta tcgtcaccac caccgataat ggtggctcga
     4201 cggggcgtat tcgccgttca gaaggcggca ttgcctgggg cgatatgcgc aactgcctca
     4261 accagctgat aacggaaccg agcgtcgcct ccgcgatgtt tgaataccgt tttggtggca
     4321 atggcgaact ttccggtcat aatctcggaa acttgatgtt aaaggcgctg gatcacctta
     4381 gcgtgcggcc tctggaagcc atcaatttaa ttcgtaatct gctgaaagtg gatacgcatt
     4441 tgattccaat gtcagagcat cctgttgatc tgatggcgat tgacgatcag gggcatgaag
     4501 tttacggcga ggtcaatatc gaccagttaa ctacgccgat tcaagagtta ttgttaacgc
     4561 ctaatgtacc cgcaacgcgt gaggcggttc acgctatcaa tgaagcggat ctcatcatta
     4621 ttgggcctgg cagtttttat accagcctga tgccaattct gctgctgaag gaaatcgccc
     4681 aggcattacg ccgcacgcca gcgccgatgg tttatatcgg caatctgggg cgtgagttga
     4741 gtttacctgc ggctaatttg aagctggaaa gcaagctggc aattatggag cagtatgttg
     4801 gtaaaaaggt cattgatgcg gtcatcgtcg ggccaaaagt ggatgtctcg gcggtgaaag
     4861 agcggattgt gatccaggag gtactggagg ccagcgatat tccgtatcgt catgaccgcc
     4921 agttgttaca taacgcgctg gaaaaggcgt tacaggcttt aggttaacgg aaatccgaag
     4981 gaaaattccg gcttcctatt gaagacaaag tgcgcgttgt ttatgccgga tgcggcgtga
     5041 acgccttatc cggcctacaa accgcgcaaa ttcaatatat tgcggagaaa atgtaggcct
     5101 gataagcgta gcgcatcagg ctgttttccg tttgtcatca gtcttcttcg ctatcctgtt
     5161 acgatgccgc gataaacagc tcacgcagct gatgcaactg gtcacgaatt tgcgccgctt
     5221 cttcgaactc cagattctgc gcgtgttgca tcatcaaccc ttccagctca tggattttct
     5281 gctgcaacgc tttaggcgac atatccatcg gcacattatc cggctcaaca atcggtcgcg
     5341 attttcctct gcccttcgct ttggttttag caatgttctg ccccaacgcc aggatatcga
     5401 ccactttctt gttcaagcct tgcggcgtaa tgccgtgttc ctcgttgtac ttctgctgtt
     5461 tctcgcggcg acgttcggtt tcgccaatcg ctttcgccat tgatggggtg atcttatcgc
     5521 cgtagagaat cgctttaccg ttaacgttac gtgccgcacg accaatggtc tggatcaacg
     5581 aacgttcgga acgcaggaag ccttctttgt cagcgtcgag gatcgccacc agcgaaactt
     5641 ccggcatatc cagaccttca cgcagtaagt tgatccctac caatacgtcg aactcaccca
     5701 gacgcaagtc gcggataatc tccatacgct cgacggtgtc gatatctgag tgaagataac
     5761 gcacgcgctc accgtgttct tcgagatatt cggtaagatc ttccgccatt cgcttggtca
     5821 gtgtggtgac cagtacgcgt tcgttaattg ccgctcgctg acgaatctcc gaaagaagat
     5881 catcaacctg tgtcgccacc ggccgcactt cgataatcgg gtcaagcaat ccggttggac
     5941 gcaccacctg atccaccaca tcgccgccgg atttttccag ctcgtaatta cccggcgtcg
     6001 ccgaaacata gatggtttgc ggcgctaatg cttcgaactc ttcaaactta agcggacggt
     6061 tatccagcgc tgatggcagg cggaagccgt actccaccag tgtctcttta cgcgcccggt
     6121 caccgcgata catgccgcca atttgtggaa tggtgacgtg agattcatcg accaccagca
     6181 gcccatcagc agggaggtaa tcaaacagcg tcggcggtgg ctcacccggt ccacgaccgg
     6241 agaggaagcg cgagtagttt tcaatccccg aacagtagcc cagctcgttc atcatctcca
     6301 gatcaaactg ggtacgctgg gtcagccgct gctcttccag cagtttgttg ttttccaaca
     6361 gcactttgcg tctggcggcc agctcttctt tgatctcctc catcgcctgt acgatgcgct
     6421 cgcgcggtgt gacgtagtgc gttttcgggt agatggtaaa acgtggaata gtggaaacaa
     6481 tctgcccggt cagcgggtca aataacgaca atcgttccac ttcctcgtca aacagttcca
     6541 cgcgaagtgc aatgtcatcc gattctgccg ggaagatatc tatcacctcg ccacgaacgc
     6601 ggaaagtacc acgctggaat gcttgatcat tacgagcgta ttgcagctcc gccagtcggc
     6661 gcagaatcgc gcgctgatcg ataatcatac cgaccgtgag atggagcatc atcttgagat
     6721 ataaatcagg atcgcccaga ccataaatcg cggaaacaga cgccaccaca accacatcac
     6781 gccgctccag catcgctttg gtggcggaca aacgcatctg ctcaatatgt tcgttaaccg
     6841 aggcatcttt ctcaatgaaa gtgtcggaac tcggtacata ggcttccggc tgatagtagt
     6901 cgtagtagga aacgaaatat tccaccgcgt tttccgggaa gaactctttc atttcgccat
     6961 acagctgggc cgccagcgtt ttgttgggcg caagtaccat ggttgggcgc tgaaggtcag
     7021 caatgacatt ggcaatggtg aatgttttcc cggagccagt cacgccaagt aacgtctggt
     7081 gcgccaggcc atcttccagc ccctcttcga gacgtcgaat cgcctctggc tgatcgccag
     7141 aaggtttaaa agcggaattc agtttgaacg gtttactcat gagtcgctac ctgaaggagt
     7201 tgggcgggca ggtatgtaat tttactcgtc gtacttaatt atgccaacaa attatactgg
     7261 ataaaaaaac agttcatcac cataatattt ctgttacagc gtaaactccg ctgtcaa
//
LOCUS       DAZFJF010000188         7299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFJF010000188 DAZFJF010000000
VERSION     DAZFJF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.190"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..114)
                     /locus_tag="ACHKAU_004577"
     CDS             complement(<1..114)
                     /locus_tag="ACHKAU_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533503.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
     gene            complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHKAU_004578"
     CDS             complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHKAU_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO5533504.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHKAU_004579"
     CDS             complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHKAU_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5533505.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHKAU_004580"
     CDS             complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHKAU_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO5533506.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHKAU_004581"
     CDS             complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHKAU_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO5533507.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1712 a         1964 c         1956 g         1667 t
ORIGIN      
        1 gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc cagcaagaga
       61 gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct tcatgtaaaa
      121 ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata gaatatgcct
      181 aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa gcactatttc
      241 atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg ttattcattg
      301 cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt gccagccgat
      361 aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata gcgctctgtt
      421 cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag cgcatcagca
      481 actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt gccacaggcg
      541 cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt ggttccagtg
      601 tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg tcgtctgttt
      661 gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat gccggaaggg
      721 ataacgtcag gagattatcc tcaccttcgc cgctgattac ctgcgcatcc agcgtctggc
      781 ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat aagttatcga
      841 cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc cgcaaagcca
      901 caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc gacagcggca
      961 caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca ctgcgttttg
     1021 ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta aacagccgac
     1081 gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa aggtgcaaaa
     1141 tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca atatcctgcc
     1201 agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct ccggcaatgc
     1261 cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg tcgcgccggg
     1321 ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt aagcattctc
     1381 cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt agctgtcgta
     1441 gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg cggttgaatg
     1501 ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc tggcttaatt
     1561 gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg tcagatgatg
     1621 tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc tgcgctttgg
     1681 cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct tcaatacaat
     1741 cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcgctc atcggtaagc
     1801 gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct ttagcgacca
     1861 gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagtccttcc agccagattg
     1921 ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt cgttgataac
     1981 ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg cactgagcct
     2041 ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag gtggcgatta
     2101 aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc ttaagactgc
     2161 gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt tctaataatt
     2221 ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca gctcgcccgg
     2281 cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat cgcggtcata
     2341 gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc cgctctggac
     2401 acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa aatcgctcac
     2461 gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact ccacggcact
     2521 ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg gatcggcaac
     2581 gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac gaatgccggg
     2641 taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca ccatcgatcc
     2701 gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa tgcggctgtt
     2761 aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt gcaggttggc
     2821 gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc ggcgacggcg
     2881 atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca taatttcttc
     2941 aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca tcagatgttt
     3001 ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca gcacgtcgag
     3061 attcgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca gtcgctcaat
     3121 cacctgttgc ggaaacagag tgtgaatact gttgatccac tcaggggtgg tgagatttga
     3181 gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag
     3241 ccactccagc gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc
     3301 tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt
     3361 cagttcagac atggaaaact ccccagtttt gcagcgatgc cagtgcctgc tgttcgagtt
     3421 gttgatgatg tgcaatggcc tgtggtggac aatgcaacgg catttgcagc gccgaaacgg
     3481 gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg cgttctcgtg
     3541 gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa tcggcatcac
     3601 ttagttgatt taaatgactg ctgaaaccgg cgataaatgt cggctgacag gccagttgat
     3661 ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg gataactgcg
     3721 ccagcatcgt cagcgccgct tcggcactgg cgttaggatg ttccagacgc atcagtgcgc
     3781 catgagccgc gccgcgatcc agcgccgggg catgaattgc ctgactgcgt cgctccagta
     3841 aggctaccgc cgcagaaaga gaaatgccgg ggagttgcac accgctatgt agatcgcgca
     3901 ggatatgaca caacgcttgc cagctatgca ggtgagcgtg aaactccttt tcatctggtc
     3961 tgccgttaga ttcacatagc cacagcgtgc gatcgatagc cgcgcataac gtcgtttgta
     4021 atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca tatagcactt
     4081 ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat gtgagttgct
     4141 ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta agacatgtgg
     4201 tgatactgcc gattccaccc gcgtccagca tattggcttc taatttatgg cgtgcggctt
     4261 cctggagtgt tgcaccaaag caggcggcct caatcaatgc cgcatgctgg ctaagcgggc
     4321 gggttaattt ccagcgttct tcaccgttgc ctgcaagtgt cagtgtgctt ccctgctggc
     4381 gtacaatccc tgggatctcc agtattgcca gccgatgtaa cacctgactt tgcgctagtc
     4441 cattggggtt aaagcgattt aaggtaagct ccgccggaag agagattgcg gtacgttcca
     4501 gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt agaaggggca agtttaccga
     4561 atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta tcgggatgaa
     4621 taacgccgcg atagctccac ggcaacggcg cattcagggc ttctttaatc agcgagcctg
     4681 ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac tgtgccagcg
     4741 ccatcgcatg cagatgagcg gcagccatat ccgcggtcga agcaggcaac ttgtgctggc
     4801 gcaaacgggt aaggatagtt tttagcagtt gttcaccggc ctgctgtaag ccccactgcc
     4861 agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc agcacatcaa
     4921 ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc agcgagggca
     4981 attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt gccggagcgt
     5041 gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc catgcaatcc
     5101 agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa tcgccccgca
     5161 gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt tcatcttcga
     5221 acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc agtaaggcct
     5281 ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc cagcaaggca
     5341 aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc tgccactccg
     5401 gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg tcctgatact
     5461 ggcaataact gtaaatagct accggaagct ggtgggataa aaacagttcg tctacccggt
     5521 cattaaaatc agccgggcct ttaatcaaca cgtatcgtgg ccgctggctt tcgattaacg
     5581 atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt aacggctcgc
     5641 tcatgcatct ttcctcacgg caggcggtgg cgggcttgat aataagcctg ccagtgagct
     5701 tctttatgtg tggcgactcg ttgttcgaag taacggcgca gacgagcgcg atcttcttcg
     5761 ttatctttga cgatggttcc ggcaatgcag tccaccaggt cggctggctc acctgcgcga
     5821 ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc ttcagcggtg
     5881 gacataatcg ctgttagtgt atccattgac gttttctttt cgccgttggc acgcagatcg
     5941 cggaaggtgc ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg aataccgcta
     6001 tgggccagca aacgcgccga agcactggcg acaagctcca gctcctgagc gaaatccata
     6061 atcggaaaga cagtttcaaa atcgaagcga cgctttagcg cggcactcat ctcattgacg
     6121 ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc atagagttga
     6181 ctggcttcac cagtgagttc cggcaccgtc ataactctgt cagaaagcat acctaacaga
     6241 caatcttgca cttccagcgg tgtacgcgtt atctcttcga aacggacgat tttgccgtcg
     6301 cgcattccct ggtaaagcgg ggcagggacc agcgcttccg ttgatgggcc atgattgata
     6361 agcagagcgt agttccagcc gtacttgatt tgatcttcag tggtagatgc gccgccctgg
     6421 atggttaacc cggcatcgcc gctaatagcg gttgccagta attcagacag gagagatttt
     6481 gcggtgccag gttcccccac caacatcaaa ccgcgtccgg tggcaagagt gactaacata
     6541 cgttcgacag cggagggact aatcaccact ttacggctaa tattttgcgc gctatcgcca
     6601 aggataaaag cacgggccgc aggcaaactt aactgccaac cgggcgggca aggtgcgtta
     6661 tcattttgtt ttaattttgc cagttcatcg gcgtataaca cagcagcagg cggacgctgc
     6721 agatgattat tctgcggtga catatattca tccctgaaat gtattaatga aataaacgat
     6781 attttacgcg caataaatgt ttggcgcatt agttgtttga gcggaaaata tctggaaagc
     6841 agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta ctattttcag
     6901 gcctaacacc acagcctgtg caacatcgtg ttccaacgaa cgaattataa ccacggctgg
     6961 accccgttgc aattaagata atgagattta taatcctgac tgttgggacg ttgcgccgga
     7021 tcgaacctat ttcaagtcct ggctaccgga cggggggtgc tgcaggtggt gccctgatcc
     7081 ggagtgcaac atcgtggtcc aacatacgta aaccagtatc gacaggcagt gtaagggtaa
     7141 ggtgctggtg ttgcgctcct taataaggag agcggatgga ctcgctgaca caaaagttaa
     7201 ccgtgctcat tgctgtactg gagctattag tggctctgtt acggttgatt gatttgttga
     7261 agtaacgggc aaattgaaac gcatctaagg gggaaaacc
//
LOCUS       DAZFJF010000189         7259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFJF010000189 DAZFJF010000000
VERSION     DAZFJF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.191"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            494..1483
                     /gene="araF"
                     /locus_tag="ACHKAU_004582"
     CDS             494..1483
                     /gene="araF"
                     /locus_tag="ACHKAU_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO5533508.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1553..3067
                     /gene="araG"
                     /locus_tag="ACHKAU_004583"
     CDS             1553..3067
                     /gene="araG"
                     /locus_tag="ACHKAU_004583"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO5533509.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3082..4068
                     /gene="araH"
                     /locus_tag="ACHKAU_004584"
     CDS             3082..4068
                     /gene="araH"
                     /locus_tag="ACHKAU_004584"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO5533510.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4235..5035
                     /gene="otsB"
                     /locus_tag="ACHKAU_004585"
     CDS             4235..5035
                     /gene="otsB"
                     /locus_tag="ACHKAU_004585"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO5533511.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5010..6434
                     /gene="otsA"
                     /locus_tag="ACHKAU_004586"
     CDS             5010..6434
                     /gene="otsA"
                     /locus_tag="ACHKAU_004586"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO5533512.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6441..6869)
                     /gene="uspC"
                     /locus_tag="ACHKAU_004587"
     CDS             complement(6441..6869)
                     /gene="uspC"
                     /locus_tag="ACHKAU_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO5533513.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1848 a         1667 c         1861 g         1883 t
ORIGIN      
        1 taaaacgttt tttcattttt gcgagatggc tctcattata cgtgttctgt taattaatga
       61 gtacagtgat aattaatggc gaaatgaatg acgtgcattt tccacatctt tgagttgcgg
      121 ttattacacc atttcaaaaa ctcaacgcca ggtaatgcgg cctattgact ggttaaaaag
      181 aagacatccc gcatgggtac caaagacaac aaggatttcc aggctaatct tatggattaa
      241 tctgctgtgc attcgacaat ttgtctgaca aattggcttt cccttatgtc ttttcccgct
      301 aaatttatgc acgttctcac tgtaattctg cgatgtgata ttgctctcct atggagaatt
      361 aatttctcgc taaaactatg tcaacacagt cacttatctt ttagttaaaa ggtaatgctt
      421 tgttttccga ttaatttaac gaatgtcatt cgtttttgcc ctacacaaaa cgacactaaa
      481 gctggagaga accatgcaca aatttactaa agccctggca gccattggtc tggcagccgt
      541 tatgtcacaa tccgctatgg cggagaacct gaagctcggt tttctggtga agcaaccgga
      601 agagccgtgg ttccagaccg aatggaagtt tgccgataaa gccgggaagg atttagggtt
      661 tgaggttatt aagattgccg tgccggatgg cgaaaaaaca ttgaacgcga tcgacagcct
      721 ggctgccagt ggcgcaaaag gtttcgttat ttgtactccg gaccccaaac tcggctctgc
      781 catcgtcgcg aaagcgcgtg gctacgatat gaaagtcatt gccgtggatg accagtttgt
      841 taacgccaaa ggtaagccaa tggataccgt tccgctggtg atgatggcgg cgactaaaat
      901 tggcgaacgt cagggccagg aactgtataa agagatgcag aaacgtggct gggatgtcaa
      961 agaaagcgcg gtgatggcga ttaccgccaa cgaactggat accgcccgcc gccgtactac
     1021 gggatctatg gatgcgctga aagcggccgg attcccggaa aaacaaattt atcaggtacc
     1081 taccaaatct aacgacatcc cgggggcatt tgacgctgcc aactcaatgc tggttcaaca
     1141 tccggaagtt aaacattggc tgatcgtcgg tatgaacgac agcaccgtgc tgggcggcgt
     1201 acgcgcgacg gaaggtcagg gctttaaagc ggccgatatc atcggcattg gcattaacgg
     1261 tgtggatgcg gtgagcgaac tgtctaaagc acaggcaacc ggcttctacg gttccctgct
     1321 gccaagcccg gacgtacatg gctataaatc cagcgaaatg ctttacaact gggtagcaaa
     1381 agacgttgaa ccgccaaaat ttaccgaagt taccgacgtg gtactgatca cgcgtgacaa
     1441 ctttaaagaa gaactggaga aaaaaggttt aggcggtaag taatttgccg gaaaaattcc
     1501 cctctgcatg atgcagaggg ggtgtgaacg accagtgatt cacggagacg ttatgcaaca
     1561 gtctaccccg tatctctcat ttcgcggcat cggtaaaacg tttcccggcg ttaaggcgct
     1621 gacggatatt agttttgact gctatgccgg tcaggttcat gcgttgatgg gtgaaaatgg
     1681 cgcaggaaaa tcaactctct taaaaatcct cagcggcaac tatgcgccaa ccacgggttc
     1741 tgtagtgatt aatgggcagg aaatgtcctt ttccgacacg accgcagcac ttaacgcggg
     1801 tgtggcgatt atttaccagg aactacatct cgtgccggaa atgaccgtcg cggaaaacat
     1861 ctatctcggc cagctgccgc ataaaggcgg cattgtgaat cgctcattgc tgaattatga
     1921 ggcgggttta caacttaaac atcttggtat ggatattgac ccggacacgc cgctgaaata
     1981 tctctccatt ggtcagtggc agatggttga aatcgccaag gcgctggcgc gtaacgctaa
     2041 aattatcgcc tttgatgagc caaccagctc cctttctgcc cgcgaaatcg acaatctttt
     2101 ccgcgttatt cgtgaactgc gaaaagaggg gcgggtgatc ttatacgttt ctcaccgtat
     2161 ggaagaaata tttgccctca gcgatgccat cactgtcttt aaagatggac gttatgtcaa
     2221 aacctttacc gatatgcagc aggttgacca cgacgcgctg gtgcaggcga tggtcgggcg
     2281 cgacattggc gatatctacg gctggcaacc tcgtagttat ggcgaggagc gcctgcgtct
     2341 tgatgctgtg aaagcaccag gcgtgcgtac gccaataagt ctggcggttc gcagtggtga
     2401 aattgttggg ctgtttggtc tggtaggggc ggggcgtagc gaattaatga aaggcttgtt
     2461 tggcgggacg caaatcaccg ccggtcaggt ttatatcgac caacagccga tcgatattcg
     2521 taaaccgagc cacgccattg ccgcaggcat gatgctctgc ccggaagatc gcaaagcgga
     2581 aggcattatt cccgtgcact ccgttcgcga caatatcaac atcagtgcca gacgtaaaca
     2641 tgtgctcggc ggttgtgtaa tcaacaacgg ttgggaagaa aacaatgccg atcaccacat
     2701 tcgttcgctc aacatcaaaa cgccgggcgc tgagcaactg atcatgaatc tctcaggcgg
     2761 aaatcagcaa aaagccattc tgggccgctg gttatcggaa gagatgaagg tcattttgct
     2821 ggatgaacct acgcgcggca ttgatgttgg tgctaagcat gaaatttaca acgtgattta
     2881 tgcgctggcg gcgcagggtg tggcggtgct gtttgcctcc agcgacttac ctgaagtcct
     2941 cggcgttgcc gaccggattg tggtgatgcg ggaaggtgaa atcgccggtg aattgttaca
     3001 cgagcaggca gatgagcgtc aggcactgag ccttgcgatg cctaaagtca gccaggcagt
     3061 tgcctgagta aggagagtat gatgtcttct gtttctacat cggggtctgg cgcacctaag
     3121 tcgtcattca gcttcgggcg tatctgggat cagtacggca tgctggtggt gtttgcggtg
     3181 ctctttatcg cctgtgccat ttttgtccca aattttgcca ccttcattaa tatgaaaggg
     3241 ttgggcctgg caatttccat gtcggggatg gtggcgtgtg gcatgttgtt ctgccttgct
     3301 tccggtgact ttgacctttc tgtcgcctcc gtaattgcct gtgcgggtgt caccacggcg
     3361 gtggttatca acctgactga aagcctgtgg attggcgtgg cagcggggtt gctgcttggc
     3421 attctctgtg gcctggtcaa tggctttgtt atcgccaaac tgaaaataaa tgctctgatc
     3481 acaacactgg caacgatgca gattgttcga ggtctggcgt acatcatttc agacggtaaa
     3541 gcggtcggta tcgaagatga aagcttcttt gcccttggtt acgctaactg gttcggtctg
     3601 cctgcgccaa tctggctcac cgtcgcgtgt ctgattatct ttggtttgtt gctgaataaa
     3661 accacctttg gtcgtaacac cctggcgatt ggcgggaacg aagaggctgc gcgtctggcg
     3721 ggtgtaccgg ttgttcgcac caaaattatt atctttgttc tctctggcct ggtatctgcg
     3781 atagccggaa ttattctggc ttcacgtatg actagtgggc agccaatgac gtcgattggt
     3841 tatgagctta ttgttatctc cgcctgcgtt ttaggtggcg tttctctgaa aggtggcatc
     3901 ggaaaaatct catatgtggt ggcgggtatc ttaattttag gcaccgtgga aaacgccatg
     3961 aacctgctta atatttctcc tttcgcgcag tacgtggttc gcggcttaat cctgctggca
     4021 gcggtgatct tcgaccgtta caagcaaaaa gcgaaacgca ctgtctgatg cttttttcca
     4081 caacaattta acgttttttc ccaccacagc cagccgccac aacggttggc tgttcttcat
     4141 tgcaaatggc gacccccgtc acactgtcta tacttacatg tctgtaaagc gcgttctgcg
     4201 caaaacaata agaaaagaga aggaggagag ccgggtgaca gaaccgttaa ccgaaacccc
     4261 tgaactatcc gcgaaatatg cctggttttt tgatcttgat ggaacgctgg cggaaatcaa
     4321 accgcatccc gatcaggtcg tcgtgcctga caatattctg caaggactac agctactggc
     4381 aaccgcaagt gatggtgcat tggcattgat atcagggcgc tcaatggtgg agcttgacgc
     4441 actggcaaaa ccttatcgct tcccgttagc gggcgtgcat ggggcggagc gccgtgacat
     4501 caatggtaaa acacatatcg ttcatctgcc ggatgcgatt gcgcgtgata ttagcgtgca
     4561 actgcataca gtcatcgctc agtatcccgg cgcggagctg gaggcgaaag ggatggcttt
     4621 tgcgctgcat tatcgtcagg ctccgcagca tgaagacgca ttaatgacat tagcgcaacg
     4681 tattactcag atctggccac aaatggcgtt acagcaggga aagtgtgttg tcgagatcaa
     4741 accgagaggt accagtaaag gtgaggcaat tgcagctttt atgcaggaag ctccctttat
     4801 cgggcgaacg cccgtatttc tgggcgatga tttaaccgat gaatctggct tcgcagtcgt
     4861 taaccgactg ggcggaatgt cagtaaaaat tggcacaggt gcaactcagg catcatggcg
     4921 actggcgggt gtgccggatg tctggagctg gcttgaaatg ataaccaccg cattacaaca
     4981 aaaaagagaa aataacagga gtgatgacta tgagtcgttt agtcgtagta tctaaccgga
     5041 ttgcaccacc agacgagcac gccgccagtg ccggtggcct tgccgttggc atactggggg
     5101 cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg tgaaacaggg aatgaggatc
     5161 agccgctaaa aaaggtgaaa aaaggtaaca ttacgtgggc ctcttttaac ctcagcgaac
     5221 aggaccttga cgaatactac aaccaattct ccaatgccgt tctctggccc gcttttcatt
     5281 atcggctcga tctggtgcaa tttcagcgtc ctgcctggga cggctatcta cgcgtaaatg
     5341 cgttgctggc agataaatta ctgccgctgt tgcaagacga tgacattatc tggatccacg
     5401 attatcacct gttgccattt gcgcatgaat tacgcaaacg gggagtgaat aatcgcattg
     5461 gtttctttct gcatattcct ttcccgacac cggaaatctt caacgcgctg ccgacatatg
     5521 acaccttgct tgaacagctt tgtgattatg atttgctggg tttccagaca gaaaacgatc
     5581 gtctggcgtt cctggattgt ctttctaacc tgacccgcgt cacgacacgt agcgcaaaaa
     5641 gccatacagc ctggggcaaa gcatttcgaa cagaagtcta cccgatcggc attgaaccga
     5701 aagaaatagc caaacaggct gccgggccac tgccgccaaa actggcgcaa cttaaagcgg
     5761 aactgaaaaa cgtacaaaat atcttttctg tcgaacggct ggattattcc aaaggtttgc
     5821 cagagcgttt tctcgcctat gaagcgttgc tggaaaaata tccgcagcat catggtaaaa
     5881 ttcgttatac ccagattgca ccaacgtcgc gtggtgatgt gcaagcctat caggatattc
     5941 gtcatcagct cgaaaatgaa gctggacgaa ttaatggtaa atacgggcaa ttaggctgga
     6001 cgccgcttta ttatttgaat cagcattttg accgtaaatt actgatgaaa atattccgct
     6061 actctgacgt gggcttagtg acgccactgc gtgacgggat gaacctggta gcaaaagagt
     6121 atgttgctgc tcaggaccca gccaacccgg gcgttcttgt tctttcgcaa tttgcgggag
     6181 cggcaaacga gttaacgtcg gcgttaattg ttaaccccta cgatcgtgac gaagttgcag
     6241 ctgcgctgga tcgtgcattg actatgtcgc tggcggaacg tatttcccgt catgcagaaa
     6301 tgctggacgt tatcgtgaaa aacgatatta accactggca ggagtgcttc attagcgacc
     6361 taaagcagat agttccgcga agcgcggaaa gccagcagcg cgataaagtt gctacctttc
     6421 caaagcttgc gtaggagcgg ttaatctccc gtaagtggaa ctaacagcac atcgacctca
     6481 cttgaggcaa taactctttt cgccgagcag gatgctcgcg aaaagaaact gtgattgtga
     6541 ttaccgcaaa tcaccaaatc gaaatgatgt ttgtgacata cttccagaat atgttcgctt
     6601 aactcaccgt aggcaataaa cgtcttgtca acgggatacc ccgcatcctg aattaactta
     6661 tcaagaaagc tttgcgtttc ttcttgcatc acgctgcgta aatcttcaag catcggcgcg
     6721 gctaattgat tgtacatttc cgggtcggaa gcgagagtaa ttaaactgat gtgtcccttt
     6781 actggcctgg cgatagatac cgcttttgcc agcagttgct gactttccgg tgtaaccgca
     6841 acagcgacaa gaatattgct atagctcata acctgttcct tattctgtga acttcaggtg
     6901 acattaaagc tttactccta atggagaaac gacgcaatcc caactcggtc aaacaggtaa
     6961 atattagtct gattattagt aaagacaatt aatcaatcac tcccggtgat catataattt
     7021 caaatgtaat tataagaaaa tagtgtactg attctccgca ttaaacaagt ggttaatata
     7081 tgtaaaacat ttcattaaca ttaagttgat tgttgccttt ctttgtattt aattagtttg
     7141 ttgtgcggta agtgtctgtt taaaaatagc ttatctggta ttgcatgaaa gtgattattt
     7201 atagcagatg attatttacg gtgagttatt ttaactgtgc gcaacatccc atttcgatt
//
LOCUS       DAZFJF010000190         7253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFJF010000190 DAZFJF010000000
VERSION     DAZFJF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.192"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..240
                     /gene="yafQ"
                     /locus_tag="ACHKAU_004588"
     CDS             <1..240
                     /gene="yafQ"
                     /locus_tag="ACHKAU_004588"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO5533514.1"
                     /translation="KDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWK
                     GYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            396..1136
                     /gene="dpaA"
                     /locus_tag="ACHKAU_004589"
     CDS             396..1136
                     /gene="dpaA"
                     /locus_tag="ACHKAU_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO5533515.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1107..1874)
                     /gene="yafJ"
                     /locus_tag="ACHKAU_004590"
     CDS             complement(1107..1874)
                     /gene="yafJ"
                     /locus_tag="ACHKAU_004590"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO5533516.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2080..2658)
                     /gene="lpcA"
                     /locus_tag="ACHKAU_004591"
     CDS             complement(2080..2658)
                     /gene="lpcA"
                     /locus_tag="ACHKAU_004591"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO5533517.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2898..5342
                     /gene="fadE"
                     /locus_tag="ACHKAU_004592"
     CDS             2898..5342
                     /gene="fadE"
                     /locus_tag="ACHKAU_004592"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO5533518.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5385..5831)
                     /gene="ivy"
                     /locus_tag="ACHKAU_004593"
     CDS             complement(5385..5831)
                     /gene="ivy"
                     /locus_tag="ACHKAU_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO5533519.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6012..6779
                     /gene="yafV"
                     /locus_tag="ACHKAU_004594"
     CDS             6012..6779
                     /gene="yafV"
                     /locus_tag="ACHKAU_004594"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO5533520.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(6900..6995)
                     /locus_tag="ACHKAU_004595"
                     /pseudo
     CDS             complement(6900..6995)
                     /locus_tag="ACHKAU_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7003..>7253)
                     /locus_tag="ACHKAU_004596"
     CDS             complement(7003..>7253)
                     /locus_tag="ACHKAU_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5533521.1"
                     /translation="TAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIA
                     INILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1702 a         1835 c         1932 g         1784 t
ORIGIN      
        1 aaggatgtaa aacttgcaca aaagcgtcat aaggatatga ataaattgaa atatcttatg
       61 acgcttctta tcaataatgc tttaccgctt ccagctgttt ataaagacca cccgctgcaa
      121 ggttcatgga aaggttatcg cgatgctcat gtcgaaccgg actggatcct gatttacaaa
      181 cttaccgata aacttttacg atttgagaga actggaactc acgcggcgct ctttgggtaa
      241 agcacgctgc cgatgaaatt tgtaaaccct gcgccgacag gcttgcgtaa aactcttttg
      301 gtgataatgt gagccttcca aatccagact attccgattt gggatctatt ttgccgtcct
      361 ggcgtgtgta accgttttat caaggaataa gcagtatgcg taaaatcgca ttaattcttg
      421 cgatgttgtt gatcccgtgc gtttcgtttg ccggtttgct gggcagcagt agctcgacca
      481 cgccagtgag caaagagtat aagcagcagt tgatgggatc ccctgtctac atccagatct
      541 tcaaggaaga acgtacgctc gatctctacg tcaaaatggg cgagcaatat cagctgctcg
      601 acagttataa aatctgtaaa tattccggcg gcttagggcc aaaacagcgt cagggcgatt
      661 tcaaaagccc ggaagggttt tatagcgtcc agcgtaatca gttaaaacca gacagccgtt
      721 actacaaagc gattaatatt ggtttcccca atgcctatga ccgtgcgcat ggttatgaag
      781 ggaaatacct gatgatccac ggcgattgtg tttccatcgg ctgctacgca atgaccaatc
      841 agggtattga tgagatcttc cagttcgtta ctggtgcgct ggtgtttggt cagccgagcg
      901 tgcaagtgag tatttacccg ttccgcatga ccgacgccaa tatgaagcgc cataaatatt
      961 ccaactttaa ggacttctgg gagcagctga agccgggcta cgactacttt gagcaaaccc
     1021 gtaagccacc gaccgtttct gtggtcaatg gccgttacgt ggtcagcaag ccgttaagcc
     1081 acgaagttgt gcagccacaa ctggcatcaa actacacgct ccccgaggca aaataagcgc
     1141 cattcgcctg gcataatctt ttgccaggtt tcattgcccg tcagcggctg tgttgcaatc
     1201 accgtgacca catcattcgg tgtggtctgc gagctgaagt cgatttccac atcctgatcc
     1261 agcagcgttg ccacgccaaa cggcgcgcgg cgggtgatcc agtgtaaatt agtcgagcaa
     1321 tacgccatca catagcgccc gtcagaaagc agcatgttga aaacgccctt ctgccgcagt
     1381 tcatccgcca gtgaggcgat atatttaaac actgccgcca tgttgcccgg tgtgcgcggg
     1441 taacgctgcg ttaatttatg caggagccag cagaaggctt tttcgctatc ggtttcaccg
     1501 accgggcgga agttgccggt ttccagtgat ttgtagcccg tcagttgtcc gttatgggca
     1561 taagtccagt tacgccccca taactcgcgg gtaaatgggt gagtattttc cagcgccacc
     1621 tcgccccgat tagcctggcg aatatgagcc accaccgaac aggattttat cgggtagtcc
     1681 tggacaagtt tggcgatggg ggaattaaag ctgggttgtg gatctttaaa tgtgcgacag
     1741 cctttacctt cgtaaaaggt aatgccccag ccatctttat gtggcccggt tccaccacca
     1801 cgctgtacaa gtccggtgaa actaaagcag atatcggtag ggacgttggc gctcatcccg
     1861 agcaattcgc acatgggtaa cctccacaac agcgggagcg aatggccccc ggcgaagatt
     1921 gatttaagaa ttttcaggtc ggatgcggcg taaacgaatt attcggccta cggttcgggt
     1981 acagatttgt aggcatgata agacgcgtca gcatcgcatc aggcatcagc gcacaagtgc
     2041 cggatgcggc gtaaacgcct tatccagcct acgccagact tacttaacca tctctttttc
     2101 aattaactgg atcaggatat ggatcacttt aatgtgaatc tcctgaatgc ggtcggcata
     2161 accaaagtgc ggtacgcgaa tttcgatatc cgccgtgcca gccattttgc cgccgtcttt
     2221 accggtcagg gtgatcactt tcatcccctt ctcacgcgcc gctgcgatcg ctttgatcac
     2281 gtttgcagag ttaccggagg tggagatccc cagcagtaca tcgccttcgc gacctaccgc
     2341 ttcaacgtag cgggagaaaa tatcattgaa accgaaatca ttaccgacgc aggaaatatg
     2401 actaacgtca gaaatagcaa tcgccgggta gcccggacgg ttttcacggt agcgaccggt
     2461 caactcttcg gcaaagtgca tagcgtcgca atgggaaccg ccgttgccgc aggaaagcac
     2521 tttgccaccg gctttaaagc tgtctgctaa caggaccgcc gcgcgctgaa tggcgtgaat
     2581 attggcgtca tcttttaaaa agttagccag cgtttccgcc gcttcgttca gttcgttacg
     2641 aataagatcc tggtacatga ggatatcctt cagcataaat gtaatagaca aaatgcagtg
     2701 taccggatac cgccaaaagc gagaagtacg ggcaggtgct atgaccagga ctttttgacc
     2761 tgaagtgcgg ataaaaacag caacaatgtg agctttgttg taattatatt gtaaacacat
     2821 tgctaaatgt ttttacatcc actacaacca tatcatcaca agtggtcaga cctcctacaa
     2881 gtaaggggct tttcgttatg atgattttga gtattctcgc tacggttgtc ctgctcggcg
     2941 cgttgttcta tcaccgcgtg agcttattta tcagcagtct gattttgctc gcctggacag
     3001 cctccctcgg cgttgctggt ctgtggtcgg cgtgggtact ggtgcctctg gccattatcc
     3061 tcgtgccatt taactttgcg cctatgcgta agtcgatgat ttccgcgccg gtatttcgcg
     3121 gtttccgtaa ggtgatgccg ccgatgtcgc gcactgagaa agaagcgatt gatgcgggca
     3181 ccacctggtg ggagggcgac ttgttccagg gcaagccgga ctggaaaaag ctgcataact
     3241 atccgcagcc gcgcctgacc gccgaagagc aagcgtttct cgacggcccg gtagaagaag
     3301 cctgccggat ggcgaatgat ttccagatca cccatgagct ggcggatctg ccgccggagt
     3361 tgtgggcgta ccttaaagag catcgtttct tcgcgatgat catcaaaaaa gagtacggcg
     3421 ggctggagtt ctcggcttat gcccagtctc gcgtgctgca aaaactctcc ggcgtgagcg
     3481 ggatcctggc gattaccgtc ggcgtgccaa actcattagg cccgggcgaa ctgttgcaac
     3541 attacggcac tgacgagcag aaagatcact atctgccgcg tctggcgcgt ggtcaggaga
     3601 tcccctgctt tgcactgacc agcccggaag cgggttccga tgcgggcgcg attccggaca
     3661 ccgggattgt ctgcatgggc gaatggcagg gccagcaggt gctggggatg cgtctgacct
     3721 ggaacaaacg ctacattacg ctggcaccga ttgcgactgt gcttgggctg gcgtttaaac
     3781 tctccgaccc ggaaaagctg ctgggtggcg cagaagattt aggtattacc tgtgcgctga
     3841 tcccgaccac cacgccgggc gtggaaattg gtcgtcgcca cttcccgctg aacgtaccgt
     3901 tccagaacgg accgacgcgc ggtaaagatg tcttcgtacc gatcgattac atcatcggtg
     3961 ggccgaaaat ggccgggcaa ggctggcgga tgctggtgga gtgcctctcg gtaggccgcg
     4021 gcatcaccct gccttccaac tcaaccggcg gcgtgaaatc ggtagcgctg gcaacgggcg
     4081 cgtatgctca cattcgccgt cagttcaaaa tctctatcgg taagatggaa gggattgaag
     4141 agccgctggc gcgtattgcc ggtaatgcct acgtgatgga tgctgcggca tcgctgatta
     4201 cctacggcat tatgctcggc gaaaaacctg ccgtgctgtc ggctatcgtt aagtatcact
     4261 gtacccaccg cgggcagcag tcgattattg atgcgatgga tatcactggt ggcaaaggca
     4321 ttatgctcgg gcagagcaac ttcctggcgc gtgcttacca gggcgcaccg attgccatca
     4381 ccgttgaagg ggccaacatt ctgacccgca gcatgatgat cttcggacaa ggagcgattc
     4441 gttgccatcc gtacgtgctg gaagagatgg aagcagcgaa gaacaatgac gtcaacgcgt
     4501 tcgataaact gttgttcaaa catatcggtc acgtcggtag caacaaagtt cgcagcttct
     4561 ggctgggcct gacgcgcggt ttaaccagca gcacgccaac cggcgatgcc actaaacgct
     4621 actatcagca cctgaaccgc ctgagcgcca acctcgccct gctttctgat gtctcgatgg
     4681 ctgtgctggg cggcagcctg aaacgtcgtg agcgcatctc ggcacgtctg ggggatattt
     4741 taagccagct ttacctcgcc tctgccgttc tgaagcgtta tgacgacgaa ggccgtaatg
     4801 aagccgacct gccgctggtg cactggggcg tacaagatgc gctgtatcag gctgaacagg
     4861 cgatggatga tttactgcaa aacttcccga accgcgtggt tgccgggctg ctgaatgtgg
     4921 tgatcttccc gaccggacgt cattatctgg caccttctga caagctggat cataaagtgg
     4981 cgaagatttt acaagtgccg aacgccaccc gttcccgcat tggtcgcggt cagtacctga
     5041 cgccgagcga gcataatccg gttggcttgc tggaagaggc gctggtggat gtgattgccg
     5101 ccgacccaat tcatcagcgg atctgtaaag agctgggtaa aaacctgccg tttacccgtc
     5161 tggatgaact ggcgcacaac gcgctggcga aggggctgat tgataaagat gaagccgcta
     5221 ttctggtgaa agctgaagaa agccgtctgc gcagtattaa cgttgatgac tttgatccgg
     5281 aagagctggc gacgaagccg gtaaagttgc cggagaaagt gcggaaagtt gaagccgcgt
     5341 aattgaataa cggagccgaa aggctccgtt tctttatccg ctaattattt aaaattaaag
     5401 ccatccggat ggttttccag gctgccggtc aacgccgcga acagcaccgt tttaccatca
     5461 atcgaaagcg catcgttcac attcagccag gtgagtttct cttgcgacgt tttctcatca
     5521 atagtcgaga acagccccgt catctgatta gatttctcgg accacatcac agcgatacgt
     5581 tgcgagccac agtcatgcgg tttgcacgcg ctcatcacct gatacgtctc atctcccaac
     5641 gttacggttt gtgcgggagt ataagtaccg cctttcatca cccaggcagg cagcttatgc
     5701 ccttgtacca tctgattaaa tgcagctttg gtggtttcgc cctttgcaag gctgctaatg
     5761 gttaaatcat cctgcgccat tgcactggtg gcgatgacca gagcggcgac tgtcgttatt
     5821 gccttaaaca tcattcctcc cgagcttatc ctgcccatgt tattaacctt ctaaagcctg
     5881 gcgataacac gataatttgc aaatcagata tttccaatat cagcatttgt tatcaattta
     5941 ttctgcgcta aggcaactgc ctgataaggt gttaaagtta taaaaccctt cgcataaagg
     6001 agttgtacac cgtgcctggt ttgaagatta cgcttttgca gcaaccactg gtgtggatgg
     6061 atggccctgc caacctgcgt cattttgatc gtcaactgga aggtattacc gggcgcgatg
     6121 tgatcgttct accggagatg tttaccagcg gctttgccat ggaagcggca gcttcgtcgc
     6181 tagcacaaga tgacgtagtg aactggatga cagctaaggc gcagcagtgc aatgcgctga
     6241 ttgcaggcag tgttgcatta caaacggagt ctggttcggt taaccgcttt ttgctggttg
     6301 agccgggcgg cacggtacat ttttatgata agcgtcatct gttccgcatg gcagatgagc
     6361 atctacatta taaagcgggc aatgcgcgag tgattgtgga atggcgcggc tggcgtattt
     6421 tgccgctggt gtgctacgac ttacgttttc ctgtgtggtc gcgcaatctc aacgattatg
     6481 acctcgccct gtacgtcgcc aactggcctg ctccgcgctc tctgcactgg caggcattgc
     6541 tgacggcccg cgcgattgag aatcaggcgt atgtggcggg atgcaatcgc gtcggcagcg
     6601 atggcaacgg ctgccattat cgcggtgaca gccgggtgat taatccacaa ggcgagatta
     6661 tcgctactgc cgacgcgcat caggcaacgc gcattgatgc cgagctgtcg atggcggtgt
     6721 taagggaata tcgcgataag tttccggcat ggcgggatgc ggatgagttt aggttgtagt
     6781 gagtgggggc tgtatttagg gcggggaatt cagaaaggga tgtttagtgt gctgagcgag
     6841 agttaaataa taaaaaactg ggaagattta ataatccccc cgcagggcaa gagcatgaat
     6901 taaatttgtg ttaaattttt cattttatca gttcgaaacc caagctctga gcaggatgca
     6961 agcacttctt ctatataaca tgtgtttaac gccagggcaa gattacgaaa gcccgctccc
     7021 cgcaaggact gacgccagat agtttctgtc catggctgct tttcgcatct tacgtcttaa
     7081 ccctgccttg aataccttat cattcgtcaa aatattaata gcaatgtgcc gtatccctga
     7141 aaataattct gctgcatttc ctcttcttat tttgcagtcg tcttcattca ttaccacgtc
     7201 cagacgccag tgcagcttat tctccacgtg ccagtgattt cggatcgctg tgg
//
LOCUS       DAZFJF010000191         7248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFJF010000191 DAZFJF010000000
VERSION     DAZFJF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.193"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..1644)
                     /locus_tag="ACHKAU_004597"
     CDS             complement(52..1644)
                     /locus_tag="ACHKAU_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO5533522.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1863..2783
                     /gene="ldtB"
                     /locus_tag="ACHKAU_004598"
     CDS             1863..2783
                     /gene="ldtB"
                     /locus_tag="ACHKAU_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5533523.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YLAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2842..3960)
                     /locus_tag="ACHKAU_004599"
     CDS             complement(2842..3960)
                     /locus_tag="ACHKAU_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO5533524.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3957..4424)
                     /gene="mntR"
                     /locus_tag="ACHKAU_004600"
     CDS             complement(3957..4424)
                     /gene="mntR"
                     /locus_tag="ACHKAU_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO5533525.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4610..4738
                     /gene="mntS"
                     /locus_tag="ACHKAU_004601"
     CDS             4610..4738
                     /gene="mntS"
                     /locus_tag="ACHKAU_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO5533526.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5010..6593
                     /gene="opgE"
                     /locus_tag="ACHKAU_004602"
     CDS             5010..6593
                     /gene="opgE"
                     /locus_tag="ACHKAU_004602"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5533527.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6642..7157)
                     /gene="ompX"
                     /locus_tag="ACHKAU_004603"
     CDS             complement(6642..7157)
                     /gene="ompX"
                     /locus_tag="ACHKAU_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO5533528.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         1831 a         1951 c         1718 g         1748 t
ORIGIN      
        1 tttgtcatca gccgccgtca ccccgacagt atcggggtga cggataatca cttactcgat
       61 ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc gttccggggt
      121 gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg aaacaaagat
      181 cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt ccatatccag
      241 gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa
      301 cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga tgtcgtcctg
      361 gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt
      421 ccactgactc atccattcga acacggtcag atcattttca aactcatatt cgtggtcctg
      481 ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt ccggttgcag
      541 atcgcccacc agcgttttca gcagcgttga tttaccgacg ccgttggtac ccagtaccgc
      601 aagtttttca ccgacctcca gcagcaggtt gagattttta aacagtggac cgttatcaaa
      661 ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa
      721 acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat
      781 ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa agcggctaac
      841 gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca gcagacgttc
      901 acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa cgcgcagctc
      961 gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga
     1021 gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc
     1081 gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggatcag caaacagcgc
     1141 ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg
     1201 ctgttccact ggaattccca cgccaagcaa caattcaccg gcgcgagctt ccgcagagta
     1261 accgtccatt tcgccgtatt taacttccag atcggccact ttatagccgt cttcctcact
     1321 catttccggc aaagcataga tgcggtcgcg ctcctgcttc acttcccaca actctttatg
     1381 ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag
     1441 cttaccaatg cgctcgttgg gatcgaggga aacgttaccc aaagtcggct ctaagtcgcc
     1501 gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg
     1561 gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat
     1621 ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca
     1681 ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa
     1741 ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat
     1801 gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta
     1861 acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca
     1921 gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga
     1981 atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgcggagt
     2041 accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga
     2101 aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag
     2161 gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg accaacaccg
     2221 ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc aactggacca
     2281 ccaaagttga gcgtaagaaa gcaggcccga cctggacgcc gaccgccaaa atgcacgcag
     2341 agtacctcgc tgcgggcgaa ccgcttccgg ctgtcgttcc ggcaggtccg gataacccga
     2401 tgggtctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc aacgccaact
     2461 tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat
     2521 tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag
     2581 cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg tctaccaccg
     2641 aagcccagtt tgaaggtcag gaaattgtgc caattaccct gacgaagagc gtgcaaacag
     2701 tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgattaaa aaccgctccg
     2761 ggatgccggt tcgtctgaat taatcttcaa aacttaaagc aaaaggcgga ctcataatcc
     2821 gcctttttta tttgccagac cctagttggc cgggagtata actaacaaaa catatcccac
     2881 caacgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg
     2941 atcgttcgcc atacgtagcg caatcaaatt tgccagcgat ccgggtaata acccaaagcc
     3001 acctacgttt accgcccata ccagtaataa agacggcggc acatagttca gcaacaatat
     3061 ggtactcggc acgttactga tcacctgcga taaaccgatt gccgttaacc ataacccggg
     3121 ttcagataga tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa
     3181 atggacgtcg ataaacatcg ccataaacac cagcagcagc gtccagtcca cactgagcac
     3241 cacgcgacgt gccagcagcg caaagcctgc cgccacaatc accagtcccc acagctcttg
     3301 tttgaactcc agcgccgtca gaaagacgat atacagcccc agacaactcc acaccagccg
     3361 cggtttccac tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca
     3421 acaccagcac aggagcagga gcgtcagcat cattgcgcca gccagcggtg ccatttgggc
     3481 aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc
     3541 aattggcgtc agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt
     3601 aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc
     3661 gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag
     3721 ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct
     3781 taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc
     3841 gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac
     3901 aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat
     3961 ttggcaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg
     4021 tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag
     4081 aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc
     4141 gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta
     4201 agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga
     4261 gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg
     4321 cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac
     4381 tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct tcctcattat
     4441 ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt
     4501 acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta
     4561 atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt
     4621 caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc
     4681 tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttccg ataaatagcg
     4741 gcgtcgcggt acgccgcttc actcctgctt tcatgcaggc ataacgcgtt ttggtctgaa
     4801 aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt
     4861 gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat
     4921 gcggacccct ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa
     4981 gcacatggcg tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta
     5041 cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta
     5101 acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc
     5161 ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtatc agttcgctgg
     5221 tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaacttc aatacattgc
     5281 tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt
     5341 acagctacat ggtcggcata tttatttttg tgctcggcgt aatagcaatc aggcgaaaaa
     5401 aagagagtga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg
     5461 cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag
     5521 atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga
     5581 ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg
     5641 ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg tgaaagcgcg cgtcgcgatg
     5701 ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt
     5761 tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt ggcttaacgc
     5821 tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc
     5881 gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg
     5941 ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg
     6001 aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc
     6061 aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct
     6121 gcgacaggac acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc
     6181 tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca
     6241 gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg
     6301 gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt
     6361 ttatggtcat ttccagcgac gataaagcgc atcgggtgat taaagcccgc cgctcagcca
     6421 atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca
     6481 aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac
     6541 agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaacaaaa
     6601 aatccgcccc gagaggcgga ttttttatat caccaaagtg attagaagcg gtaaccaaca
     6661 ccggcaatcc aggtacctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc
     6721 agagcaacgt tttccatcgg gttgaactgc agaccagcac cgtaggagaa accgtagtcg
     6781 ctggtgtcgt gtttgtaggt cgggtattcc gtggtctgga atttaccata acccaccccc
     6841 actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg
     6901 tagtactggt ttttgttgta gtcaccagag cttgcagtac ggcttttctc ggtgtaagtg
     6961 aaagagccga tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa
     7021 ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacggta
     7081 gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga
     7141 catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata
     7201 tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacggg
//
LOCUS       DAZFJF010000192         7146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFJF010000192 DAZFJF010000000
VERSION     DAZFJF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.194"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(313..1379)
                     /locus_tag="ACHKAU_004604"
                     /pseudo
     CDS             complement(313..1379)
                     /locus_tag="ACHKAU_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(1382..1744)
                     /locus_tag="ACHKAU_004605"
     CDS             complement(1382..1744)
                     /locus_tag="ACHKAU_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5533529.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2281..4194
                     /gene="mtlA"
                     /locus_tag="ACHKAU_004606"
     CDS             2281..4194
                     /gene="mtlA"
                     /locus_tag="ACHKAU_004606"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO5533530.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4424..5572
                     /gene="mtlD"
                     /locus_tag="ACHKAU_004607"
     CDS             4424..5572
                     /gene="mtlD"
                     /locus_tag="ACHKAU_004607"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5533531.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5572..6159
                     /gene="mtlR"
                     /locus_tag="ACHKAU_004608"
     CDS             5572..6159
                     /gene="mtlR"
                     /locus_tag="ACHKAU_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO5533532.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6171..6380)
                     /gene="yibT"
                     /locus_tag="ACHKAU_004609"
     CDS             complement(6171..6380)
                     /gene="yibT"
                     /locus_tag="ACHKAU_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO5533533.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6665..7027
                     /locus_tag="ACHKAU_004610"
     CDS             6665..7027
                     /locus_tag="ACHKAU_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO5533534.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1814 a         1795 c         1818 g         1719 t
ORIGIN      
        1 aaatattttt taaatgtgac tgtcatcctt cagataataa agccatcatc gcaatagatg
       61 gaaaagcact cctgctcttt tttgattaga gccgtcgcag gaggaacaac tcatatcatc
      121 attgcgatct cgacgatgta tagtctcatc ctgcggcaga acaagacgga taaaaaatcc
      181 aatgagagca cactcatcca gaacttatta acataatgaa tattaaagaa aaatcatcgc
      241 agctaatgaa gtaatatgca aaaataatta gtccttacaa ggagcaaagt tccataatct
      301 cccccctccc cctcaatgat ccaaataaag ataatgcatc cagctggtca ttcttagcag
      361 cactttccgc atcaccgaaa catggctgaa atggtcggag taaaccgcca cctgggcgta
      421 gatgccgtcg ggtaaggcat cgatatcatc gttagggtcc agttcaatgg ttcccagcac
      481 accgtccgtg ccgggcacga ccgttaatga ttgcaatacc ccctgcgcct gataagaacc
      541 gcctggcacg acaggtaaaa tactggtcag tttgccgtgg aacacctgcc caggtagcgc
      601 gttaaacacc acttctgcat catcaccagg tttcagacgt aacagcgagt tttgccgaaa
      661 ttgggcgaca atttgccgtt tttgctcggg gatgaaaacc atcaccggac gcaacggcaa
      721 ggcagctgcg tatgtacctg ggcggatcag tacctgagta atgtagccat tgctcggcgc
      781 gcgaatgaca gtctgctcaa gattatattt tgcttcagta agttgcgctc ttaagctcac
      841 aatctgcgat tgctcgccgt taaccatact gtcgagctgg ctctggatct gcgcctgctc
      901 cgccaccgag cctttcacca gcgcatcctg cgcgaggaaa ttttgccgcg catcgtcgat
      961 gtcacgttcc gagaacggat tcaccgccgc ctggctgcct ttcagataac gttgataatt
     1021 tttaaacaga cggtcgcgct ccgctgaaac ctgggtggtg ttggcctgcg cttctgtgag
     1081 ctgcgcgcgc agagtcttta tattatgcgt cgccgtcatc aggtcagcct gaagcctgtc
     1141 aactcgcgcc tggtaacgaa ccgggtcgag cttaaaaagc acctcgccct tttgaataag
     1201 ctgattattc ttgtcagtga cttcagtaac aattcccgtc acctgtggcg tgatggggat
     1261 cgctatcact gccttttgcg cggtaaaagt gtaagggtgg ttgtagttca tcaacaaaat
     1321 caaaccactc accagaaaca cgccagcgcc acgtaagtta aaacaatcaa tagatccata
     1381 attagatctc cgggaacgtg gaatagtcag tctttttctc ggcggcgagt tgatgctcca
     1441 gccgggaaat tcggtcagaa agtgcggcga tttccgggtc agtcgctttc tcctgcgacg
     1501 caacatgtga ctgcataccc cagccacgct ccggttgata gagcgtcgcc cagatccaca
     1561 gaaacggcca gataacatgc agagtaaaca ggctcaccca gcccgccgta tgaatagcgt
     1621 cggcatgggg atggttgcgc tttttggcaa tcagataagg gatgtcatgt atcgcgatga
     1681 ttccataaaa aatcaccaga aagacgaaga tcagcactcc caacgcgaaa tagtttagaa
     1741 acatatctgc ctcggattca cgtttatcag tgttgttttt gggctggcag ccagaaggga
     1801 gtcaggctga tattttgaca ataatccggg ttcgcgattc tcgccataac accaaagaat
     1861 aatttttaga ggtgatgagt tgcttagtta cataacgatt gtatgacgaa ggcataacat
     1921 gctgtagatc acatcaggtg aacgccgtaa gaaaatatct tgtgattcag atcacaaaga
     1981 ttcaacaaac catcaaaaca aaaatgtgac actactcaca tttaaatgcc atttttagcg
     2041 aaaatcgccg ccttgttgct tttttacaca agcgttttgt gatgaacgtc acgtcaatta
     2101 cctctctacc ccctatattt atgtgattga tatcacacaa aaggccgtcg actggacagt
     2161 taaccgattc agtgccagat ttcgcagtat ctacaaggtc cggctacctc tgccgccaca
     2221 ttaacaaaaa acctcgggct tccagcctgc gcgacagcaa acataagaag gggtgttttt
     2281 atgtcatccg atattaagat caaagtgcaa agctttggtc gtttcctcag caacatggtg
     2341 atgccaaata tcggcgcgtt tatcgcgtgg ggtatcatca ccgcgttatt tattccaaca
     2401 gggtggttac cgaacgagac gctggcgaag ctggtcgggc cgatgatcac ttatctcctg
     2461 ccgctgctga tcggttatac cggtggtaag ctggtaggcg gcgaacgtgg cggcgtagtc
     2521 ggtgccatca ccaccatggg cgttatcgtc ggcgcagaca tgccgatgtt cctcggttct
     2581 atgattgcag gtccgctggg cggctggtgc attaagcact tcgaccgctg ggtagacggt
     2641 aagatcaaat ccggttttga gatgctggtg aataacttct ccgcaggcat catcgggatg
     2701 atcctcgcta ttctggcatt cctcggcatt ggcccgattg ttgaagccct gtccaaaatg
     2761 ctggctgcgg gcgttaactt catggttgtc catgacatgc tgccgctggc gtctatcttt
     2821 gttgaaccgg cgaaaatcct gttcctcaac aatgccatta accacggtat cttctcgccg
     2881 ctgggtattc agcagtccca tgaactgggt aaatcaatct tcttcctgat tgaagctaac
     2941 ccaggtccag gtatgggcgt gctgctggcg tacatgttct ttggtcgtgg tagcgctaaa
     3001 cagtctgcgg gcggtgcggc aatcatccac ttcctgggtg gtatccacga aatctacttc
     3061 ccgtatgtgc tgatgaatcc gcgtctgatc ctcgcagtca tcctcggcgg tatgactggc
     3121 gtgttcacgc tgactatcct gggcggtggt ctggtttctc cggcatctcc gggttctatc
     3181 cttgctgtac tggcgatgac accaaaaggt gcttatttcg ctaacatcgc gggtgtgtgt
     3241 gcggcgatgg ctgtctcctt cgttgtctct actattttgc tgaaaaccag caaagtgaaa
     3301 gaagaagatg atattgaagc agcaactcgt cgtatgcagg acatgaaagc tgagtctaaa
     3361 ggcacatctc cgctgtctgc gggcgatgtg actaacgacc tgagccacgt acgtaaaatc
     3421 atcgttgcct gtgacgccgg tatgggttca agtgcgatgg gcgcaggcgt gctgcgtaag
     3481 aaaattcagg atgcaggtct gtcacagatt tcggtcacta acagcgcgat caacaacctg
     3541 ccgccagatg tggacctcgt catcactcac cgtgacctga ccgaacgcgc tatgcgccag
     3601 gttccgcagg cgcagcatat ttcgctgacc aacttcctcg acagcggcct gtacaccagc
     3661 ctaaccgaac gtctcgttgc tgcccagcgc catactgaaa acgaagtgaa agtgaaagac
     3721 agtctgaaag acagctttga cgattccagt gctaacctgt tcaaactggg cgcggagaac
     3781 atcttcctcg gtcgcaaagc agcaaccaaa gaagaagcga ttcgttttgc tggcgagcag
     3841 ctggtgaaag gcggttacgt tgagccggaa tacgttcagg cgatgctgga tcgtgaaaaa
     3901 ctgaccccga cttatctggg tgagtctatc gcggtgccac acggtacggt tgaagcgaaa
     3961 gatcgcgtac tgaaaacggg cgtggtgttc tgccagtacc cggaaggcgt gcgcttcggt
     4021 gaagaagaag atgacattgc ccgtctggtg attggtattg cagcccgtaa caacgagcac
     4081 attcaggtta tcaccagcct gaccaatgca ctggatgatg agtccgtcat cgagcgtctg
     4141 gcacacacca ccagcgtgga tgaagtgctg gaactgctgg caggtcgtaa gtaatccaat
     4201 cccaccctct ccacatggag aaggtggggt taattgcctg atgcgctacg cttatcaggc
     4261 ctacaggatg catcacaatt tgttgaattt gcacgttctt gtaggccgga taaggcgctt
     4321 acgccgcatc cggcgctgcc cctctcctca cggagagggt ttgggtgagg gaaaagcctc
     4381 accccagccc tctcgggtaa aaacattgat gaaggttaat actatgaaag cattacattt
     4441 tggcgcaggt aatatcggtc gtggctttat cggtaaactg ctggcagacg cgggtatcca
     4501 actgacgttt gccgatgtca atcaggtggt acttgatgcc ctgaatgccc gtcatagcta
     4561 tcaggttcat gtggttggtg aaaccgagca ggtagatacc gtttccggcg tcaatgctgt
     4621 cagcagcatt ggtgatgatg tcgttgatct gattgctcag gtcgatttag tcactaccgc
     4681 cgttggcccg gttgtgctgg aacgtattgc tccggctatc gccaaagggc tggtgaaacg
     4741 caaagaacaa ggtaatgaat ccccgctgaa catcatcgcc tgtgaaaaca tggtacgcgg
     4801 taccacgcag ctgaaaggcc atgtgatgaa cgccctgccg gaagacgcca aagcgtgggt
     4861 agaagaacac gttggctttg tcgattccgc cgttgaccgc atcgtaccgc cttcggcttc
     4921 ggcaactaac gatccgctgg aagtgacggt agaaactttc agcgaatgga ttgtcgataa
     4981 aacgcagttc aaaggcgcac tgccgaacat cccaggcatg gagttaaccg acaacctgat
     5041 ggcatttgtc gaacgtaaac tcttcaccct gaacacgggt catgctataa ccgcgtacct
     5101 cggaaaactg gccggtcatc agaccattcg tgacgctatt ctcgacgaga aaatccgcgc
     5161 ggtggtaaaa ggtgcgatgg aagaaagtgg tgcggtactg atcaagcgct acggctttga
     5221 cgcagacaaa catgcggcgt acatccagaa aatcctcggt cgttttgaga acccgtatct
     5281 gaaagatgat gtagagcgcg taggccgtca gccgctgcgt aaactgagtg ctggcgaccg
     5341 tctgatcaag ccactgctcg gtacgctgga atacagcctt ccgcacaaga atctgattca
     5401 ggggattgct ggtgcaatgc acttccgcag tgaagatgat ccgcaggctc aggaactggc
     5461 agcactgatc gctgacaaag gtccgcaggc ggcgctggca cagatttccg gtcttgatgc
     5521 caacagcgag gttgtatccg aggcggtaac cgcttataaa gcaatgcaat aatggtggac
     5581 caggcgcagg acaccctgcg cccgaataac agattgtcag atatgcaggc aacaatggaa
     5641 caaacccagg cctttgaaaa ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga
     5701 agctttctga tcaccgccgt cgagcttttg accgaggcgg taaatcttct ggtgcttcag
     5761 gtattccgca aagacgatta cgcggtgaag tatgctgtag aaccgttact cgacggcgac
     5821 ggtccgctgg gcgatctttc tgtgcgttta aaactcattt acgggttggg cgtcattaac
     5881 cgccaggaat acgaagatgc ggagctgctg atggcattgc gtgaagaact aaatcacgac
     5941 ggcaacgagt acgcctttac cgacgacgag atccttggac cctttggtga actgcactgc
     6001 gtggcggcgt taccaccgcc gccacagttt gaaccagcag actccagttt gtatgcaatg
     6061 caaattcagc gctatcaaca ggctgtgcga tcaacaatgg tcctttcact gactgagctg
     6121 atttccagaa tcagcttaaa aaaagccttt caaaagtaag caacgtctgc ttactgcccc
     6181 tctacctgct tcggccgata aagccgacga taatactcca gacgttgtag atataacggc
     6241 acgctttcat cgggtattcc ggacggaatc gcgttacggg gaggaagttt tttcagatac
     6301 tcccggaacg cctggcttga tgccatgaaa tctacggctt tatcgataag aagcggaacg
     6361 ttttcaccta atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc
     6421 cgccaatttt agtttaggtt cccggtaaag atgacattaa ggaaaacgtg ctgaatcctc
     6481 aataatcatc cgctgctgtt tattatcttt tttctataga tttcttaatt aatcaacgaa
     6541 ttgcgtagtt tttgagcatg ttaatgatta cgttgcagaa ctattacaat gtcatcagtt
     6601 gtgaacaaag cacctggtcg cgcatactag gggctataaa tttatcttta tcagaagcca
     6661 tcacatgaaa gaagtcgaaa aaaacgaaat caaacgtctc agcgatcgcc tggacgccat
     6721 ccgccaccag caggccgatc tgtcgctggt tgaagccgca gacaaatatg ccgagctgga
     6781 aaaagagaaa gccacgctgg aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa
     6841 actgagtaaa gaagcacaga agctgatgaa gatgccgttt cagcgcgcaa ttaccaaaaa
     6901 agagcaggct gatatgggca agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc
     6961 aatgaccgcg ctgggccgtg aaatgggcct gcaggagatg actggattct ctaagactgc
     7021 gttttaagcg cctcagacga ctggttcgat catatcaacc cgcatctggc ttgccttagc
     7081 tggtcaatgc ggggactctc ctgtctactc gcctaattac caaacaattc actcccttta
     7141 cgtcga
//
LOCUS       DAZFJF010000193         7144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFJF010000193 DAZFJF010000000
VERSION     DAZFJF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.195"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(156..1019)
                     /locus_tag="ACHKAU_004611"
     CDS             complement(156..1019)
                     /locus_tag="ACHKAU_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO5533535.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(991..2518)
                     /locus_tag="ACHKAU_004612"
                     /pseudo
     CDS             complement(991..2518)
                     /locus_tag="ACHKAU_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            2776..3555
                     /gene="deoC"
                     /locus_tag="ACHKAU_004613"
     CDS             2776..3555
                     /gene="deoC"
                     /locus_tag="ACHKAU_004613"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO5533536.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3682..5004
                     /gene="deoA"
                     /locus_tag="ACHKAU_004614"
     CDS             3682..5004
                     /gene="deoA"
                     /locus_tag="ACHKAU_004614"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO5533537.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            5056..6279
                     /gene="deoB"
                     /locus_tag="ACHKAU_004615"
     CDS             5056..6279
                     /gene="deoB"
                     /locus_tag="ACHKAU_004615"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5533538.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6359..7078
                     /gene="deoD"
                     /locus_tag="ACHKAU_004616"
     CDS             6359..7078
                     /gene="deoD"
                     /locus_tag="ACHKAU_004616"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO5533539.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1659 a         1896 c         1958 g         1631 t
ORIGIN      
        1 cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg cgttcacgcc
       61 gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac
      121 aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc cggaaatatc
      181 aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc ttccggcgtg
      241 gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc gttcaggcgt
      301 accggaacat cgccaagtcc cttgataaac accgccagtt cttcgatgtg ttgcaaataa
      361 tccacctggc cagggattac cagcaaacgc agttccgcca gcttaccgcg ctctgccagc
      421 aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgttg atgacattcg
      481 ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa tttttcccag
      541 ccggtttcgc tcaacatacc gttactgtcc acgaggcagg tgaggtggcg cagttgcgga
      601 tcgtttttga tggctgtaaa cagcgccacc acaaacggca gctgggtcgt ggcttcgccg
      661 ccgcttaccg ttatcccttc gataaacagc accgctttgc ggatatggct aagcacctca
      721 tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacatctt caggcaggta
      781 tcacactgct cgcagaccac agcgttccac agcactttgc cgtcaacaat ctgcaacgcc
      841 tgatgcggac actgtggcac gcactctccg caatcattgc agcgtcccat cgtccacgga
      901 ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag acgactgcct
      961 ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct gctgttcatg
     1021 gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt cttcgcccag
     1081 ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg acaaacgcac
     1141 catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa attcacgcat
     1201 tccggcttta aaggcaccga ggcaaagctg taccagtgcc tgcgggttac gtttgatggt
     1261 ttcgtcgagg gtcagaatgt cgctgatgcc gggcgacagt ttgcagatgg gtgatcggat
     1321 ctggctcatc gccatacggc aaacgcgcgc ccggcgtggt gccgatatcg gaactgatcc
     1381 ccgactgtgc gtgtaacatg gcgcgttttt gccagccata tttcacgggg gtattggcga
     1441 caaactccgc cagttgcgcg ctgatgcgat aacccacttc atttgcggcg gcttctttac
     1501 cgtagcgcgc ggcaatccct tctttctcac acagcaagtt aaccgcttcc gccagcccat
     1561 acatgccaaa cattggcaca aaacgttcag ggttaatcag cccttctttc accaggaagc
     1621 tattctcaaa gaagtgtgat tgttgataga ggaattcaca ccgcgcatcg atgatggcga
     1681 tctgctgctg gcagtagtgc ggtagagtgc gcgtaaagaa gtcatccagc gattcgctgc
     1741 gctcggcaat ggctttcagg ttaaggcgta ccagcgtgct gccaccaccc gccagcggca
     1801 gtgagttgta acagctcaca atcccgtagc ccccttttgt gaaaatttta tcatgcaccg
     1861 gaccgttggc gatgtgcggt ttgctacatt cacagatgtt cttcgccact tccagcagca
     1921 ggtcatcagg ggtgatttct ggatcgtaga taaaggtcag gttcggtgaa acctgcttca
     1981 actctgcatc tgcacgtaat atcgcacggg tgatgggcga atcagacggg ccgatattgg
     2041 cgtgcataaa ggcgtctggc agggttctgt cgaggtaacg ccagaaacgt tttattcgaa
     2101 tatcgatctc gtcttgtgtt agaattctaa catacggttg caacaacgca tccagttgcc
     2161 ccaggtagac cggcatcgat gtgaccgacg gtacgtggtg gtaaagaatg gtcagcagag
     2221 agagtgcgtc atcaagatct ttcgcgcctt ccagctccag ccattcggaa ccgttcgcca
     2281 gaaaacgggc gtaatcgggt aagacatagc gcggtttgta cggcgcatga ccttcaaaca
     2341 tatcgcagat tacaccctca tccagcgcgc ggcgggcctc ggcaggcagc tgcggataag
     2401 gcagattgtt ttctgcttcc agtgccagaa aatggcgctt ctgctccggg ctaagcactg
     2461 ggctggtgac aatttgctgg caacgttgtt gcagtgcatt ttcatgagaa gtgggcatct
     2521 tcttttcctt ttatgccgaa ggtgatgcgc cattgtaaga agtttcgtga tgttcacttt
     2581 gatcctgatg cgtttgccac cactgacgca ttcatttgaa agtgaattat ttgaaccaga
     2641 tcgcattaca gtgatgcaaa cttgtaagta gatttcctaa attgtgatgt atatcgaagt
     2701 gtgttgcgga gtagatgtta gaatactaac aaactcgcaa ggtgaatttt attggcgaca
     2761 agccaggaga atgaaatgac tgatctgaaa gcaagcagcc tgcgtgcact gaaattaatg
     2821 gacctgacca ccctgaatga cgacgacacc gacgagaaag taattgctct gtgtcatcag
     2881 gccaaaaccc cggtcggcaa taccgccgct atctgtatct atcctcgctt tatcccgatt
     2941 gctcgcaaaa cgctgaaaga gcagggcacc ccggaaatcc gaatcgctac ggtaaccaac
     3001 ttcccacacg gtaacgatga catcgaaatc gcgctggcag aaacccgtgc ggcaatcgcc
     3061 tacggtgcag atgaagttga cgttgtgttc ccgtaccgcg cgctgatggc gggcaacgag
     3121 caggttggtt ttgacctggt gaaagcctgt aaagaggctt gcgcggcagc gaatgtactg
     3181 ctgaaagtga tcatcgaaac cggcgaactg aaagacgaag cgctgatccg taaagcgtct
     3241 gaaatctcca tcaaagcggg tgcggacttc atcaaaacct ctaccggtaa agtggctgtg
     3301 aacgcgacgc cggaaagcgc gcgcatcatg atggaagtga tccgtgatat gggcgtagaa
     3361 aaaaccgttg gtttcaaacc ggcgggcggc gtgcgtactg cggaagatgc gcagaaatat
     3421 ctcgccatcg cagatgaact gtttggtgct gactgggctg atgcgcgtca ctaccgcttt
     3481 ggtgcttcca gcctgctggc aagcctgctg aaagcgctgg gtcacggcga cggtaagagc
     3541 gccagcagct actaagtaag atgctttacg cctgatgcgc tgcgcttatc aggcctacga
     3601 gacgtatcta cccgtaggcc ggataaggcg tagacgcatc cggcaaaagc cgcctcatac
     3661 tcttttcctc gggaggttac cttgtttctc gcacaagaaa ttattcgtaa aaaacgtgat
     3721 ggtcatgcgc tgagcgatga agaaattcgt ttctttatca acggtattcg cgacaacact
     3781 atctccgaag ggcagattgc cgccctcgcg atgaccattt tcttccacga tatgacaatg
     3841 cctgagcgtg tctcgctgac catggcgatg cgagattcag gaaccgttct cgactggaaa
     3901 agcctgcatc tgaatggccc gattgttgat aaacactcga ccggcggcgt cggcgatgtg
     3961 acttcgctga tgttggggcc gatggtcgca gcctgcggcg gctatattcc gatgatctct
     4021 ggtcgcggcc tcggtcatac tggcggtacg ctcgacaaac tggaatccat ccctggcttc
     4081 gacattttcc cggatgacaa ccgtttccgc gaaattatta aagacgtcgg cgtggcgatt
     4141 atcggtcaga ccagctcact ggctccggcg gataaacgtt tctacgcgac ccgtgatatt
     4201 accgcaaccg tggactctat cccgctgatc actgcctcga tcctggcgaa gaaactggcg
     4261 gaaggtctgg atgcgctggt gatggacgtg aaagtgggta gcggcgcgtt tatgccgacc
     4321 tacgaactct ctgaagccct tgccgaagcg attgttggcg tggctaacgg cgctggcgtg
     4381 cgcaccaccg cgctgctcac cgacatgaat caggtactgg cctccagtgc aggtaacgcg
     4441 gttgaagttc gtgaagcggt gcagttcctg acgggtgagt atcgtaaccc gcgtctgttt
     4501 gatgtcacga tggcgctgtg cgtggagatg ctgatctccg gcaaactggc gaaagatgac
     4561 gccgaagcgc gcgcgaaatt gcaggcggtg ctggacaacg gtaaagcggc agaagtcttt
     4621 ggtcgtatgg tagcggcaca aaaaggcccg accgacttcg ttgagaacta cgcgaagtat
     4681 ctgccgacag cgatgctgac gaaagcagtc tatgctgata ccgaaggttt tgtcagtgaa
     4741 atggataccc gcgcgctggg gatggcagtg gttgcaatgg gcggtggacg ccgtcaggca
     4801 tctgacacca tcgattacag cgtcggcttt actgatatgg cgcgtctggg cgaccaggta
     4861 gacggtcagc gtccgctggc ggttatccac gcgaaagacg aaaacagctg gcaggaagcg
     4921 gcgaaagcgg tgaaagcggc aattaaactt gccgataaag caccggaaag cacaccaact
     4981 gtctatcgtc gtatcagtga ataacggtat actgatctga tcatttaaat ttgaagcact
     5041 gagtacggag aacatatgaa acgtgcattt attatggtgc tggactcatt cggcatcggc
     5101 gctacagaag atgcagaacg ctttggtgac gtcggggctg acaccctggg tcatatcgca
     5161 gaagcctgtg ccaaaggcga agctgataac ggtcgtaaag gcccgctcaa tctgccaaat
     5221 ctgacccgtc tggggctggc gaaagcacac gaaggttcta ccggtttcat tccggcggga
     5281 atggacggca acgctgaagt tatcggcgcg tacgcatggg cgcacgaaat gtcatccggt
     5341 aaagataccc cgtctggtca ctgggaaatc gccggtgtcc cggttctgtt tgagtgggga
     5401 tatttctctg atcacgaaaa cagcttcccg caagagctgc tggataaact ggtcgaacgc
     5461 gctaatctgc cgggttacct cggtaactgc cactcttccg gtacggtcat tctggatcag
     5521 ctgggcgaag agcacatgaa aaccggcaag ccgattttct atacctccgc tgactctgtg
     5581 ttccagattg cctgccacga agaaaccttt ggcctggata aactctacga actgtgcgaa
     5641 atcgcccgtg aagagctgac caacggcggc tacaacatcg gtcgtgttat cgctcgtccg
     5701 tttatcggtg acaaagccgg taacttccag cgtaccggta accgtcacga cctggctgtt
     5761 gagccgccag caccgaccgt gctgcagaaa ctggttgatg aaaaacacgg tcaggtggtt
     5821 tctgtcggta aaattgccga catctacgcc aactgcggta tcaccaagaa agtgaaagca
     5881 actggcctgg acgcgctgtt tgacgccacc atcaaagaga tgaaagaagc gggtgataac
     5941 actatcgtct tcaccaactt cgttgacttc gactcttctt ggggccaccg tcgcgacgtc
     6001 gccggttatg ctgcgggtct ggagctgttc gaccgccgtc tgccggagct gatgtctctg
     6061 ctgcgcgatg acgacatcct gatcctcact gctgaccacg gttgtgatcc gacctggacc
     6121 ggtactgacc acacgcgtga acacattccg gtactggttt acggcccgaa agtaaaaccg
     6181 ggctcactgg gtcaccgtga aaccttcgcg gatatcggcc agactctggc aaaatatttt
     6241 ggtacttctg atatggaata tggcaaagcc atgttctgat aatgggtgcg gcttgatgcc
     6301 ctcaccccgg ccctctccca cagggagagg gtgaaaacat gtagataagg ataaaacaat
     6361 ggctacccca cacattaatg cagaaatggg cgatttcgct gacgtagttt tgatgccagg
     6421 cgacccgctg cgtgcaaagt atattgctga aactttcctt gaagatgccc gtgaagtgaa
     6481 caacgttcgc ggtatgctgg gcttcaccgg tacttacaaa ggccgcaaaa tttccgtaat
     6541 gggtcacggt atgggtatcc cgtcctgctc catctacacc aaagaactga tcaccgattt
     6601 cggcgtgaag aaaattatcc gcgtgggttc ctgtggcgca gttctgccgc acgtaaaact
     6661 gcgcgacgtc gttatcggta tgggtgcctg caccgattcc aaagttaacc gcatccgttt
     6721 taaagatcat gactttgccg ctatcgctga ctttgacatg gtgcgtaacg cggtagacgc
     6781 ggctaaagca ctgggcgttg atgctcgcgt gggtaacctg ttctccgctg acctgttcta
     6841 ctctccggac ggcgaaatgt tcgacgtgat ggaaaaatac ggcatcctcg gcgtggaaat
     6901 ggaagcggct ggtatctacg gcgtggctgc agagtttggc gcgaaagccc tgaccatctg
     6961 caccgtgtct gaccacatcc gcactcacga gcagaccact gccgctgagc gtcagaccac
     7021 cttcaacgac atgatcaaaa tcgcactgga atccgttctg ctgggcgata aagagtaatt
     7081 gtgtttcgct gaaaggcgaa cgtcgtgtta agccggagca ggagactgct ccggcttttt
     7141 agta
//
LOCUS       DAZFJF010000194         7120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFJF010000194 DAZFJF010000000
VERSION     DAZFJF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.196"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(280..765)
                     /gene="sixA"
                     /locus_tag="ACHKAU_004617"
     CDS             complement(280..765)
                     /gene="sixA"
                     /locus_tag="ACHKAU_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO5533540.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(968..3112)
                     /gene="fadJ"
                     /locus_tag="ACHKAU_004618"
     CDS             complement(968..3112)
                     /gene="fadJ"
                     /locus_tag="ACHKAU_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO5533541.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3112..4422)
                     /gene="fadI"
                     /locus_tag="ACHKAU_004619"
     CDS             complement(3112..4422)
                     /gene="fadI"
                     /locus_tag="ACHKAU_004619"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO5533542.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4602..4886)
                     /gene="yfcZ"
                     /locus_tag="ACHKAU_004620"
     CDS             complement(4602..4886)
                     /gene="yfcZ"
                     /locus_tag="ACHKAU_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO5533543.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5258..6598
                     /gene="fadL"
                     /locus_tag="ACHKAU_004621"
     CDS             5258..6598
                     /gene="fadL"
                     /locus_tag="ACHKAU_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO5533544.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6963..>7120
                     /locus_tag="ACHKAU_004622"
     CDS             6963..>7120
                     /locus_tag="ACHKAU_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533545.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLEL"
BASE COUNT         1812 a         1880 c         1660 g         1768 t
ORIGIN      
        1 tcaagtttca attacatcct gcctgattat tcaactctat tttctctatt atctgtatta
       61 ataaatgaca atgagtgtca aacagacatt attcatttca acatgtaact ttgggcaaaa
      121 tcaagatacg cgcagaagaa taatttccga atgaaagtag aaagtgtttt atttatagat
      181 ggggagagtc ttcgatagta gtgaggttga cagatatccg ctgcctgatg agcaattatg
      241 cccaatgaat ctacctcatt gggcataatt tggaactcat cagatagctt ttgccatctt
      301 cagattacac gggctcatct gccagttgaa tgtgccgttt ccgctttcat caagcgttac
      361 gctggcaatg gccgaggtgg taaacatagg cggcgtttcg cccggacata actcggcaac
      421 cagatacccg actaacggta agtgagagat caccagtacc gaagcaacac cttcattggt
      481 caacgcctgc aaataggcac tgaccagacc aacatcgccg cagggcgtta actccggcag
      541 aacttctgca ctggaaggca ggttcagaca atcccctact tcttccagtg tttgctcggc
      601 tcgcaggaac gggctcacca gaacgcgttc gatttccact ttttgacctt tcagccagtt
      661 cgccatcagg cgagattcgt cacaaccatt agtggtcaga ggacgaacgg aatcactggc
      721 ggcatcgagg gctgcgtcgc cgtgacgcat gataaaaact tgcatattgc accgcttttg
      781 ttaaccagat tcgcccgttt tctttcatct gaaaccagag aaagaaaacg gtggccggca
      841 ttgtgcctta tccattcacc gaatgaaacg ctgtttttta cctcaatggc gtaagtatag
      901 tcaatatgcg tttatatttt gaccaacact ccgccattca gcgcggattc atatagcttt
      961 gaccttctta ttgcaggtca gttgcagttg ttttccaaaa actttcccca cgcgcgccca
     1021 tctcgaccaa acgctcgcaa ggggtaaaac gggaaccata ctgcgtggca agtcgttgca
     1081 ttattgcaac cacttcgccc gcgccgagag aatcgatata gcggaacggt ccaccgagaa
     1141 atggcggaaa accaatgcca aataccgcac caatatcccc gtcacgcacg ctacggataa
     1201 cctgctcatc aacacaacgt actgcttcat tcagcatcaa catcacacac cgttcagcaa
     1261 cctgcggtgc ggagagtcgc ccctgccctt gtgcgccaat gagcgggtaa atggcgggat
     1321 cgacctgttt tttgctttta cgccctttct gaccataaag atagaaaccc cgaccatttt
     1381 ttctgccttt gcgatcgtcg ttcaaaattg aagaaacaac atttgcaggc gcgctaaaac
     1441 gttctccata agcggcttcc agtacaggaa taattttagt cccggtgtcg attcctacct
     1501 catccaaaag ttggattgga cctaccggaa aaccaaattt cactagcgcg gcatcaatgt
     1561 gctctacccg ttcaccttcg gtcagcatgc ggatagcttc attaatgtaa ggcgctaaga
     1621 tgcgattgac gtaaaacccg gctttgtcac gcacgacaat gggcgttttg ccctgttttt
     1681 tcgccagttt taccgtagtg gcgatggttt gcgccgatgt acccgcatga ggaataatct
     1741 ctaccagcgg cattttttcc accggactga agaaatgcag gccaataact tgctcaggtc
     1801 gcgtggcgtg agctgcgata tcaccaatcg gtaaagatga cgtattcgaa gcaaaaatgg
     1861 tatgagcggc gcaattttgc tccacttccg ccaccatctg ttgttttaat tcgagatttt
     1921 caaacaccgc ttcaataatc agatcgcgat gggcaaagcc gcgatagtcc gtcgttccgg
     1981 agattaatgc cagctgtttg tcacgttcgc tggctttgag atgacgacgg cgaactttgc
     2041 cctccagctg atcccaactg tacttcagcg catgatttat gccctgcggg ttgatatctt
     2101 taattctgac cggaagcccc gctttacaag cagtgacata agcaataccg ccgcccatca
     2161 agccaccacc taaaattccc acgctgttta atggcgcagg cggtgcatca ctaccgggat
     2221 ctttcttcac ttccgtactg gcaaaaaaga tactacgcaa agcttgcgat tgtggcgtca
     2281 tcgccagttc gccaaacgcc cgagcttcgg cgtcataacc gctgctggtg ccctgcgcta
     2341 atcccgtttc aacaacctcc aggatgcgtt ctgtcgccgg ataattgcct tgagttttgt
     2401 gttctgtttt cttgccgacc attttgaaca gcagcgcacg acctaacggc cccgccagaa
     2461 tacgctcgcg tacaggtaga gggcgggaag atgggcgatc cttcttcgcc agctcaacag
     2521 cgacttccag caaaatggag tgcggaacaa cgtcatccac cagccccagc tttaatgcct
     2581 gtttcgcccg aagttgtttt ccggtgagga tcatctctaa tgctgtgctg acgcctatca
     2641 gacgcggtaa acgctgggtg ccgcctgaac cgggtaacaa tccaagttgt acttcaggca
     2701 aaccgagcac cgttttagga tcgtcagtac aaacgcgacc gtggcacgcc agcgccaact
     2761 ccagcccacc acccaggcaa gcgccatgaa tagccgcgat aaccggaatg ggcaaagcat
     2821 gaatctccgc catcaactgt tggccctgcc gcgccagtgc ttccgcttct tgcgccgttt
     2881 tgcagttgcc gatcatgttg atgtctgcgc cagcaatgaa gttgtccggt ttagcggaga
     2941 caaacaccac gcctcgcaac tctttgtttt cacggagttg cttaataatg gcgcgcacct
     3001 gcgaggcaaa ctccgccttc agggtattca ttttctcacc cggtacgtcg atggtgataa
     3061 cggcaatgtt gtccagacga acattaaggg taaacgctga tgtcatttcc attattccgc
     3121 ctccagaacc attgccgcgc caagcccacc ggcagcacag gcggtaacta aaccaaatcc
     3181 accgccgcga cggcgaagtt catgcaatgt ctgggtaatc atccgtgcgc cggtcgccgc
     3241 gaagggatgt ccgtaagcaa tcgaaccgcc aagcacgtta aatttgctat cgtccacttc
     3301 gccagtggca tgtgcacgcc ccagtacttc acgagcaaaa cgttcactac ccagcaactg
     3361 aatattcgcc agtgtctgag ctgcaaaggc ttcgtgcata tcgatcaatg tcagatcgct
     3421 cattgtcaaa ccggcacgtt ccagcgccag cggtgttgac caggccggac cgagcaacat
     3481 gtcctgccag acatcaatcg cagtaaatgc gtagctgcgc agatacccca gcggcaccag
     3541 ccctaattct ttcgcccggg attcagtcat caggatcacc gctgccgcgc catcggtcag
     3601 cggcgtactg tttgccgccg ttaccgttcc gtgtttgcga tcaaacgccg ggcgcagctt
     3661 tgcgtaatcg gcaagcgagg aattaccgcg aatattgttg tcttcgacaa gcggttgttt
     3721 ataaggaggg ataaaggcag tcatcacctc ttctttgagt tttccgtctg accatgcctg
     3781 agcggcacgc tgatgcgaac ggtgcgctaa tgcatcttgc tgttctcggg tgatgccgta
     3841 ggttttcgcc atttgttcgg cggtgtcgcc catccgcaag ccagtagaat attctgctac
     3901 cgcaggcggt acgggcatta agtcgcgcaa acgcaggcga gagaagagtt tcagtcgctg
     3961 gctcatggtt cgagctttgt tgacatcaac cagcacgcgc gccagttttt tactgacgcc
     4021 aattggcaat acggaagagg aatctgcccc accggcaatc cccgctcgga tagttcccgc
     4081 catcaggctt tctgcgacgt ttgcaaccgc ctggaaactg gtagcgcaag cgcggctgac
     4141 gctgtaagca tcggtatgca cattcattcc ggtaccgaga acaatttcac gcgcaatgtt
     4201 gggggcttca ggcatttgta cgacctgacc aaagaccagc tgttcaatca cttcagcggg
     4261 gatctcgctg cgtgccagca gttcgcctac caccatcttc cctaaatcaa ccgcgggaat
     4321 gccatgaaaa gccgtcgcct ggcgggcaaa aggcgtacgt aaaccgctaa caatggcgat
     4381 acgatcgccc tggcgggtaa ccagcggtaa aacctgaccc ataacactcc cctgtaaaaa
     4441 aataaataaa gtggtctgac ctgatcatag tcttaaccat ttttttacat ttagccaagc
     4501 ggagaaaaag gaaagtggga gctatgacac agtgaagaca ggagaaagaa aagaaaacgc
     4561 ccctgccagc aactgacagg ggcgtacgta cccgagagga attaacgcag acccagctgg
     4621 aagatcagca tttcagcttc gcaggcaaaa gtgaaatcga tatccaggcg cacaccgtca
     4681 gactcttcag tgaaagtcgg ggtgatttta cacggttcgg attccacgct acgggctttt
     4741 tcagtcagcg ccgccagcgt ttgttctgct tctgcgcggc ttgcaaacac gcggctgtaa
     4801 gacgcggtgc agtcggagtt gtccataatg gtgccaacat ccatacagca gcaaaccggg
     4861 gtttcatcag cactacattt actcatcgtt gatttcctct gtatgtgcac ccaaggtgcc
     4921 agataaacgt tgtggatatt ttacgcttcc ggaaagtgct gctccagttg ttaattctgc
     4981 aaaatcggat aagtgaccga aatcacactt aaaaatgatc taaaacaaaa ttcacccgaa
     5041 tccgcgagtg cgcaacctcc aaattttgcc agctggatcg cgtttcttag atcatatttg
     5101 aaaaaagatg gaaacatact tgcaacattc cagctggtcc gacctatact ctcgccactg
     5161 gtctgatttc taagatgtac ctcagaccct acacttcgcg ctcctgttac agcacgtaac
     5221 atagtttgta taaaaataaa tcattgaggt tatggtcatg agccagaaaa ccctgtttac
     5281 aaagtctgct ctcgcagtcg cagtggcact tatctccacc caggcctggt cggcaggctt
     5341 tcagttaaac gaattttctt cctctggcct gggccgggct tattcagggg aaggcgcaat
     5401 tgccgatgat gcaggtaacg ttagccgtaa ccccgcattg attaccatgt ttgaccgccc
     5461 gacattttct gcgggtgcgg tttatattga cccggatgtg aatatcagcg gaacgtctcc
     5521 gtctggtcgt agcctgaaag ccgataacat cgcgcctacg gcatgggttc cgaacatgca
     5581 ctttgttgca ccgattaacg accaatttgg ttggggcgct tctattacct ctaactatgg
     5641 tctggcaaca gagtttaatg atacttatgc aggcggctct gtcgggggta caactgacct
     5701 tgaaaccatg aacctgaact taagcggtgc gtatcgctta aataatgcat ggagctttgg
     5761 tcttggtttc aacgccgtct acgctcgcgc gaaaattgaa cgtttcgcag gcgatctggg
     5821 gcagttggtt gctggtcaga ttatgcaatc tcctgccggg caaactcctc aagggcaagc
     5881 attggcagct accgccaacg gtatcgacag taataccaaa atcgctcatc tgaacggtaa
     5941 ccagtggggc tttggctgga acgccggaat cctgtatgaa ctggataaaa ataaccgcta
     6001 tgcactgacc taccgttctg aagtgaaaat tgacttcaaa ggtaactaca gcagcgatct
     6061 taatcgtgcg tttaataact acggtttgcc aattcctacc gcgacaggtg gcgcaacgca
     6121 atcgggttat ctgacgctga acctgcctga aatgtgggaa gtgtcaggtt ataaccgtgt
     6181 tgatccacag tgggcgattc actatagctt ggcttacacc agctggagtc agttccagca
     6241 gctgaaagcg acctcaacca gtggcgacac gctgttccag aaacatgaag gctttaaaga
     6301 tgcttaccgc atcgcgttgg gtaccactta ttactacgat gataactgga ccttccgtac
     6361 cggtatcgcc tttgatgaca gcccagttcc ggctcagaat cgttctatct ccattccgga
     6421 ccaggaccgt ttctggctga gtgcaggtac gacttacgca tttaataaag atgcttcagt
     6481 cgacgttggt gtttcttata tgcacggtca gagcgtgaaa attaacgaag gcccatacca
     6541 gttcgagtct gaaggtaaag cctggctgtt cggtactaac tttaactacg cgttctgata
     6601 acgcgttcgc ctggataaag tcacctgcat agcaggtgac tttaactccc ccacttcacc
     6661 gaaagtagtg tcctcattgc ttacctcctg agttttgcaa acagcctgat ggcagcttgc
     6721 cctttcaaat caataagcgg tatccacaga caaatctgaa gggaaaggca tattttcagg
     6781 cgttctgctc gtccttctca aagagtttac ttttctgaat ttccaggata ctcacccccc
     6841 tggctattgt gcgctcatac actcaaatta aagataggtt ctaaataaat gagcgttttt
     6901 tgatagtcta tttcattagg taatatatat ttgtaacaaa tcaatcaaaa tggaataaaa
     6961 tcatgctacc atctatttca atcaacaata ccagcgcaac ttacccagaa tccattaatg
     7021 aaaataacaa tgatgaagtt aatggattag tacaagagct caaaaacctt tttaatggta
     7081 aggaaggaat aagcacctgt attaaacatc tacttgagct
//
LOCUS       DAZFJF010000195         7102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFJF010000195 DAZFJF010000000
VERSION     DAZFJF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.197"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(102..287)
                     /locus_tag="ACHKAU_004623"
     CDS             complement(102..287)
                     /locus_tag="ACHKAU_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5533546.1"
                     /translation="MSSKNRTRRTTIRNIRFPNHMIEQLNIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            329..694
                     /locus_tag="ACHKAU_004624"
     CDS             329..694
                     /locus_tag="ACHKAU_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5533547.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAASGMPD
                     PTLPEVSGGSGRADDITGL"
     gene            985..1548
                     /locus_tag="ACHKAU_004625"
     CDS             985..1548
                     /locus_tag="ACHKAU_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5533548.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREVAAIPQPDENNPEMPPPAVMS
                     HGIEYMEDGLPDPVKAMGQILVENLSIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            1545..3206
                     /locus_tag="ACHKAU_004626"
     CDS             1545..3206
                     /locus_tag="ACHKAU_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5533549.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            3270..5207
                     /locus_tag="ACHKAU_004627"
     CDS             3270..5207
                     /locus_tag="ACHKAU_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5533550.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVTANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGTASGG"
     gene            5252..5473
                     /locus_tag="ACHKAU_004628"
     CDS             5252..5473
                     /locus_tag="ACHKAU_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533551.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            5419..7102
                     /locus_tag="ACHKAU_004629"
                     /pseudo
     CDS             5419..7102
                     /locus_tag="ACHKAU_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT         1590 a         1759 c         2253 g         1500 t
ORIGIN      
        1 tgtgtttttt caggctatcc cgtcagacgg gaagcctgta ctgccggggg acgaatggaa
       61 aactgatgtg tccggtaact gtgtgttctg tgaacaccat gttacttaat tatgtaattc
      121 atacccgaac gctctgttga cagccttctt ctgcaggctt caataaccca cgctgaaaag
      181 ttaccggaac ctttatgttc aagggcgata ttgagctgtt caatcatgtg attggggaaa
      241 cggatattgc ggattgtggt tctgcgggtc cggtttttcg atgacatttt ctttcctctg
      301 gtgacaagct atatggagag gattttacat ggctgtgctt cgtacgttac cgggcagaat
      361 caaaactctg aacacccggc gggtgaatat tctgaagggt gaacagcgtc gtgtcagtgg
      421 cagtgcacgt gtttccctca agcgtcatat ctggctcagg gatgccgggc agtgctgtct
      481 ctgtggtcgt gtggttgacc tctgtgacag tgaactcgat caccgaattg cacttcagtt
      541 cggtggtggt aatgaggaga cgaatctctg gacgctctgt accgaatgcc atcgacaaaa
      601 gtctgctcgt gaagcggcga gtggtatgcc agacccgacg ctgccggagg tgtccggagg
      661 tagtggcaga gcggacgaca tcaccggact gtaacccgac ccgggggggg tatcatccgg
      721 cgtaaaaaaa gatcgctttg gacaccgccc cccctctcac gcagagaaaa aattcccgtt
      781 tcagggcagt taacatgtta actggctgtc cgggcatttt tgcggttttt atctttatta
      841 ttcagtttgt tgtgcggaaa aaatgttaac tggctttttc agcaaatgtt aaccaggcag
      901 cagttaacat ttgcggcatg agacgccggg aaaaatgggc tgaaccatac ccggctgagt
      961 gcgttatgga cccgggagga ggctgtgctg acaacgcaaa aacgaaaatt tgcgctggcg
     1021 ctcatgtccg ggaaaaacaa aacagcgtca gccattgccg ccggttattc ggcgaagacc
     1081 gccagggtta aaggctcgca gctggcaaaa gatccggagg tgctcgcgtt tatagcccgt
     1141 aaacagtgcg aaacggtgga ggtggatgag gttcctgttt accggcagaa aaaatcagag
     1201 caggaggata aaccccgtcg ccgtgaggtg gctgcaatac cacagccgga cgaaaacaat
     1261 ccggagatgc caccgcccgc ggtgatgtct catggtattg aatatatgga ggatggtctt
     1321 cccgatccgg tgaaagctat ggggcagatc ctggtggaaa acctcagcat tgatccgaaa
     1381 ctggcactgg atgcggcctg gcgactggcg cagttcacac accataaaaa aggcgatgcc
     1441 ggtaaaaaat cggcaaaagg tgatgccgcg aaaaaagcgg ctaaccgttt tgcggtacca
     1501 ccaccaccac gactggtggt gaataacgat aatgagggca acggatgata cctgtatgga
     1561 gcacggcctg cccggactgg gcagagcgcc tgaaaaaggg gctgtcgatt attccggctc
     1621 cgatttatcc ggaccaggcc gcacatgccc tggcgatttt taaacaactg cggattgtgg
     1681 atgcaccggg tagcccgacg ttcggggagt cctgtgcacc gtgggtgttt gacctggtgg
     1741 cggctctgtt tggctcctac gatgcgcaga ccggtgtccg ccatatcaag gaagtgttca
     1801 ttctgatacc caaaaaaaac agcaagtcca cactggccgc cgggatcatg atgacggcgc
     1861 tgttactgaa ctggcggcag gcggcgggtt acacgattct ggccccgact gtggaggtgg
     1921 cagccaacgc cttcaatcct gccagggata tggtacgacg ggacgatgat ctggatgacc
     1981 tctgtcaggt gcagacacat atccggacca tcacccaccg ggtgacggac accaccctga
     2041 aggtggtggc agccgatccg aatacggtgt ccggtatcaa gtccgtgggg acgctgattg
     2101 atgagttgtg gctgtttggc aagcagtaca aggcggagga catgttacgt gaagccatcg
     2161 gcgggcttgc ctcccgcccg gaagggtttg tggtgtatac gaccacccag tcgaatgaac
     2221 cgccagccgg ggtgttcaga cagaaactgc agtacgcccg ggatgtgcgc gacggcaaaa
     2281 ttaatgatcc gcactttctg ccggtgatat ttgaacaccc tcctgaaatg gtggaaagcg
     2341 gggctcacct gctgatggaa aacctcgcca tggtcaatcc gaatctcggc tattcagtgg
     2401 atgaggcctt tctgtaccgg gagtaccgta aagcccggga agccggggaa gagacattcc
     2461 gggggttcat gtcaaaacac gccaatgtgg aaattggtct tgccctgcgc tctgaccgct
     2521 gggcgggggc tgatttctgg gaagagcagg gccgttgtat cagcctggac gatatcctgc
     2581 gtcgtgctga tgtggtgacg gtggggattg acggcggagg gctggatgat ctgctgggga
     2641 tgtatgtgat tgggcgtgac cgggagaccc gcgaatggct gggctggggc catgcctggg
     2701 cgcatgaaac cgcggtggtc cgacggaaga gcgaggcgtc ccggtttcag gatcttgttg
     2761 cctgtggaga tatgaccatt gtccggcgtg tcggggatga cacggcggaa gtggcggagt
     2821 atgtgcgtcg cattcatgag gctgagttac tggaccatat cggtattgac ccgtcagggg
     2881 tggggcagat tctggattca ctggcggaag ccgggatccc cgacggaatt gtggtgggga
     2941 taagccaggg ctggaaactg ggcggggcca ttaaaaccac cgagcgcaaa ctggctgaag
     3001 gggtgctggt gcatggtgac cagcccctga tggcctggtg tgtcggcaat gcccgggtgg
     3061 agcctaaagg taacgccatt cttatcacca aacaggccag tggacgggga aaaattgacc
     3121 cgctgatggc gctgttcaat gcggtctccc tgatgtccct taacccggaa ccgaaaaaga
     3181 aagaatatgc ggtttttttc atataaccct gttcacactg taaccatcac gaaccgctcc
     3241 ggcggttttt ttattttcag gaggctgatg tgactcttaa acgggcctgt tccctgctga
     3301 cggtgaaatc cttcagtgag gatgaacggg tgatcaccgg gattgcgtca acgccttctc
     3361 cggatcggga tggtgacatc ctggagccgg agggcgcgga gtttggcagt gcgatcccgt
     3421 ttctctggca gcatgaccat tcccgcccgg tggggcagtg tacggtacgc cgggtcagcg
     3481 aagggctgga aatcacggca acactggtga agcccgtacc ggatatgccg tcgcaactgg
     3541 ctgcccggct ggatgaggtc tgggcggcca ttaagaccgg gctggtcagg gggctgtccg
     3601 tgggcttccg tccccatgaa tacacctttc tggacggagg cggactgcat tttctgcgct
     3661 gggaactgat ggaggtgtct gccgtcaccg tgcccgcgaa tgcggaatgc accatccgga
     3721 ccattaaatc ttacgaccgc ccgttttctg ccgcgtccgg caaccggaaa ccggtggtga
     3781 aaatcgcatc ttctgccggc gctgcggcac agtcaataac ctcttttcat aaggaaaagt
     3841 cagcaatgaa tactggtgaa cagattaaaa gttttgaaaa caagcgtgcg gcgctggcag
     3901 cctcccttga ggagatcatg aacaaagccg cagaggaagg ccgcacactg gatgtggaag
     3961 aggaagagca ctacgacaac accgcagcgg aaatccgtca ggtggatgcg cacctgaagc
     4021 gtctgcgtga actggaaacc agtaaggccg ccacggcaca gccggtgaaa caggccggta
     4081 acgggaatgt ggccacggtg gcttcagcgc cggtgatccg tgttgagcag aaactggaga
     4141 aggggattgg tttcgcccgc tttgccaaat cactggccgc ggctaaaggt gtccgctctg
     4201 aagccctgga agtggcccgt cgtcagtatc cggatgacag ccgtctgcat catgtcctga
     4261 aatcggcggt gggggcaggg accaccacgg acccgcagtg ggcaggcagc ctgtctgaat
     4321 accaggaata tgcgcaggac tttattgatt acctgcgtcc gcagaccatt atcgggcgat
     4381 ttggtcaggg cgggatccct gcacttcgtc aggtgccatt caatatccgc gtgcatgccc
     4441 aggtgtccgg tggtgctgcc ggctgggtgg gtgagggtaa ggccagaccc ctgacgaagt
     4501 ttgattttga atccatcacc ttcagtcatg cgaaggtgtc ggccattgcg gtactgacgg
     4561 aagaattgat ccgtttttcc agtccggctg ctgatgcact ggtccgtaat gcgctggcgg
     4621 aagcggtggt ggcgcgtctg gacacagact ttgtggaccc gaaaaaagcg gcggtggcgg
     4681 atgtctcccc ggcgtccatc acccatgatg tgaagggcac ggcatcaacc ggtaacccgg
     4741 atgcggatgc cgaggccgcg tttggccagt ttgtgacggc aaatctgcag cccaccggtg
     4801 cggtctggct gatgtccagc accaatgccc tggcgctgtc catgcgtaaa aatgcgctgg
     4861 ggcagaagga atatccggac atgaccttgc tgggcgggac cttccagggg ctgccggtga
     4921 ttgtctccca gtatgtgggt gaccagctgg tactggtgaa tgcaccggat atttatctgg
     4981 ctgatgacgg cggtgtggcg gtggatatgt cccgtgaagc gtcactggag atgcagtctg
     5041 aaccgaccag cgacagcagc acaccgtcac cggtggaact ggtctccatg tttcagaccg
     5101 gcagcgtggc catccgtgcg gagcgctgga tcaactggcg tcgtcgccgt accgcggcag
     5161 tggcggtgat caccggtgtg aactacggaa ctgcgtccgg tggctgagtc tgatgaggag
     5221 ggcgggaggc gcgagcttcc cgcagtaaca gatggcaaaa atccagtatc tgcagggcac
     5281 gcatgatgcc cgggccgggg atatccgtga tgtggcacag ccgtgtgcgg aggtgctggt
     5341 tcgcctgggg aaggcggagt acatcacagc gcgacgtccg gcaggtcaga aaaagaaacg
     5401 tgatgcggag catggcgaat gtggaacctt ttgcggcgaa cccgaaaaaa ccagaaatca
     5461 ggacgtgacg taagagaagt gggctggcgg tccctgtttc aggcggtggc tgagcctttt
     5521 gccggtgcct ggcagcaggg tgtgaaagcc gatccggaaa ccgttttgtc ctttcacgcg
     5581 gtgttttcat gcatttcgct gatatcccag gatatcgcca aaatgcggct tcgcctgatg
     5641 cagactgatg tacagggaat acgccgggaa aagcggcagg gagatactgc ccgtctctgt
     5701 cgtcgtccta atgcgcagca gaatcgcatc cagttttttg aactgtggct gaattccaaa
     5761 ttgcgtcacg gcaatacggt ggtgctgaaa atccgcaacc cccgggggca gatcaaagaa
     5821 ctgcgtattc tggactggaa ccgggttgaa cctctggtgg cggatgacgg cgacgtgttc
     5881 taccgcatca cgccggaccg gaactgtggg atcacagagt cggtgacggt gcctgcccgg
     5941 gaggtgatcc acgaccgttt taactgtttt tttcatccgc ttgtggggct gccgccggtg
     6001 tatgccgcag gactggccgc catgcagggg catcatattc aggcaaattc gacgtatttt
     6061 ttcagaaatg gcgggcgacc atcaggcgtg attgaggtcc ccggcagtat tacggaagaa
     6121 aacgcgaaaa aactgaaggg gaactgggac agcggatata cgggcgaaaa tgccgggaag
     6181 acggccatac tgagcaacgg ggcaaaatac agccccacga cgttttcacc ggtggatgcg
     6241 cagacggtgg aacaactgaa aatgacggct gaaattgtct gttcggtgtt ccgtgtcccg
     6301 gcctacaaga ttggcgttgg ccacccgcct tccagtgaca acgtggaggc gctggagcag
     6361 cagtattatt cccagtgtct gcagacgctg attgagtcca ttgagctgtt actggatgag
     6421 gcgctggaaa cgggggaaaa cgagagcacg gagtttgacg tcaccacgct gctgagaatg
     6481 gacagcgaac ggcgcatgaa aacactgggt gaatcagtga aaaatacgct tctcacgccc
     6541 aatgaggccc gtaaacgtga gaacctgccg ccccttgccg gcggtgatgc actgtatctt
     6601 cagcagcaga actacagtct ggaggcgctg tcccgtcgtg atgcccgtga ggacccgttc
     6661 gcgtcggccg ggaaaacagt ttcagcacaa ctgcctgacg gcgcatctga cggtaataag
     6721 gcaatcagtg aaacagagca tgatgcagtg aaagcgatgt tcagggggat actgagaaaa
     6781 tgacggaacg ggaactgtcc attattcgtg cactgggcga agaattctcc acggtgctgg
     6841 cggatttaca gcgcacattt gaggggaaga tggccgcgca ggcacaagcg tttgaagaga
     6901 aactggcttc cctgtcggcg gtattacaga agcatgtgac ggtggatgag gtacatccgg
     6961 ttctgcaggc gatggtggat gacgctgtgg ggaccattcc ggtaccgcgt gatggtcgtg
     7021 attatgatcc ggatgtactg cagcaggcgg tgaatgatgc ggttgcgaat attccggtac
     7081 cggcggacgg caaaagtatc ac
//
LOCUS       DAZFJF010000196         6911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFJF010000196 DAZFJF010000000
VERSION     DAZFJF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.198"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            184..1341
                     /gene="acrE"
                     /locus_tag="ACHKAU_004630"
     CDS             184..1341
                     /gene="acrE"
                     /locus_tag="ACHKAU_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO5533552.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1353..4457
                     /gene="acrF"
                     /locus_tag="ACHKAU_004631"
     CDS             1353..4457
                     /gene="acrF"
                     /locus_tag="ACHKAU_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO5533553.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4710..4931
                     /locus_tag="ACHKAU_004632"
     CDS             4710..4931
                     /locus_tag="ACHKAU_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533554.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5362..6387
                     /locus_tag="ACHKAU_004633"
     CDS             5362..6387
                     /locus_tag="ACHKAU_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5533555.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6455..6844
                     /locus_tag="ACHKAU_004634"
                     /pseudo
     CDS             6455..6844
                     /locus_tag="ACHKAU_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
BASE COUNT         1691 a         1645 c         1768 g         1807 t
ORIGIN      
        1 aataaaacag tagaatatta atcttttttt gtgtttatgt gccttgagac gccagtattc
       61 ataactattc cttacatcga cgaatgataa tttgtaggat agcaaactgt atttttcttt
      121 ctgcgagtta acgcgttacc tttctgggta aataacgcgc ttttggtttt ttgaggaata
      181 gtaatgacga aacatgccag gtttttcctc ctgccctcct ttattctgat ctccgcggct
      241 ttaatcgccg gttgtaacga taagggagaa gagaaagctc acgtcggtga accgcaggtt
      301 accgttcata ttgtgaaaac ggccccgtta gaagttaaga ctgaattacc aggccgcacc
      361 aatgcttacc gtatagccga agttcgccca caggttagcg ggatcgtact gaatcgtaat
      421 ttcactgaag gcagcgatgt acaagcaggc cagtccctgt accagatcga tcccgcgacc
      481 tatcaggcaa attatgacag cgcgaaaggc gaactggcga aaagtgaagc cgccgccgcc
      541 atcgcgcatt tgacggtaaa acgttacgtt ccgctcgtgg gtacgaaata catcagccag
      601 caggagtacg accaggccat tgctgatgct cgccaggccg atgccgccgt gattgccgca
      661 aaagccacag tcgaaagcgc tcgcatcaat ctcgcttata ccaaagtgac tgcgccaatt
      721 agcggacgta tcggcaaatc gaccgtgacc gaaggcgctc ttgtcactaa tgggcaaacg
      781 actgaactgg cgactgtcca gcagctcgat cctatctacg ttgatgtgac ccaatccagc
      841 aacgatttta tgaggctgaa gcaatccgta gagcaaggaa atttgcataa ggaaaacgcc
      901 accagcaacg tagagttggt catggaaaac ggtcaaacct atcccctgaa aggtacgctg
      961 caattctctg atgtgaccgt tgatgaaagc accggctcca taaccctacg tgctgtcttc
     1021 cctaacccgc aacatacgct tttgccgggt atgtttgtgc gtgcacggat tgatgaaggc
     1081 gtccaacctg acgccattct tatcccgcaa caaggcgtta gccgcacacc gcgtggtgat
     1141 gcaaccgtgc tgattgttaa cgataaaagt caggtggaag cgcgccctgt cgttgccagt
     1201 caggcgattg gcgataaatg gttgattagc gaaggtctga aatctggcga tcaagtcatt
     1261 gtcagcggcc tgcaaaaagc gcgtccggga gagcaggtta aagccactac cgataccccc
     1321 gcagatactg catcgaagta aggtaatctg acatggcaaa cttttttatt cgacgaccga
     1381 tatttgcatg ggtgctggcc attattctga tgatggcggg cgcactggcg atcctacaat
     1441 tgcccgtcgc tcagtatcca accattgcac cgcctgcggt ttctgtttcg gcaaactatc
     1501 caggtgctga tgcgcagacc gtgcaggata cggtgacgca ggttatcgaa cagaatatga
     1561 atggtatcga taacctgatg tatatgtcct ccaccagcga ttccgccggt agcgtgacaa
     1621 ttacccttac cttccagtcc gggaccgatc ctgatatcgc gcaagtgcag gtgcagaaca
     1681 aactccagct cgccacgccg ttgctgccgc aggaggttca gcagcagggg atcagtgttg
     1741 aaaagtccag tagcagctat ttgatggtgg cgggctttgt ctctgataac ccagacacca
     1801 cacaggacga tatctcggac tatgtggcct ctaacgttaa agatacgctt agccgtctga
     1861 atggcgtcgg tgacgtacag cttttcggcg cacagtatgc gatgcgtatc tggctggacg
     1921 ccgatctgct aaacaaatat aaactgacac cggttgatgt gattaaccag ttgaaggtac
     1981 agaacgatca gatcgctgcc ggacagttgg gcggaacgcc agcgttacca gggcaacagt
     2041 tgaatgcctc gattattgct caaacgcggt taaaaaatcc ggaagaattc ggcaaagtga
     2101 ccctgcgcgt aaacagtgac ggctcggtgg tacgcctgaa agatgtcgca cgggttgaac
     2161 ttggcggtga aaactataac gttatcgctc gtatcaacgg aaaaccggcg gcgggcctgg
     2221 ggattaagct ggcaaccggc gcgaatgctc tcgataccgc gaaagccatt aaggcaaaac
     2281 tggcggaatt acagccattc ttcccgcagg gaatgaaggt tctctaccct tatgacacca
     2341 cgccattcgt ccagctttct attcacgaag tggtaaaaac gctgttcgaa gccattatgc
     2401 tggtgttcct ggtgatgtat ctgttcttgc agaatatgcg agcaacgctg atccccacca
     2461 ttgcggtacc cgtggtgttg ttagggacgt ttgccatcct cgccgctttt ggttactcca
     2521 tcaacacact aacgatgttc gggatggtgc ttgccatcgg gctgctcgtc gatgatgcga
     2581 tagtggtggt ggagaacgtc gagcgcgtga tgatggagga taagctcccg ccaaaagaag
     2641 cgacggaaaa atcaatgtcg caaatccagg gcgcactggt gggtatcgcg atggtgctgt
     2701 cagcggtatt tattccgatg gcattcttcg gcggttctac tggggcaatt tatcgccagt
     2761 tctctatcac catcgtttcg gcaatggcgc tttctgttct ggtggcattg attcttaccc
     2821 ctgcgttatg tgcaacgctg cttaaacccg tctctgctga gcatcacgaa aataagggcg
     2881 gtttcttcgg ctggtttaat actaccttcg atcatagcgt taaccactac accaacagcg
     2941 tcggcaaaat cctcggttcc acaggacgat atttactgat ctatgcgctg attgttgcag
     3001 gaatggtggt gttgttttta cgtcttccgt cttccttctt acctgaagag gatcagggtg
     3061 tctttctgac catgattcag ttacccgctg gcgcgacgca agagcggacg caaaaagtgt
     3121 tggatcaagt gacggattac tatctgaaga acgagaaagc gaacgttgaa agtgtcttta
     3181 cggttaacgg ctttagcttc agcggccagg cacaaaacgc cggtatggcc ttcgtcagtc
     3241 tgaaaccgtg ggaagagcgt aatggtgatg aaaacagtgc ggaagcggta atccatcgtg
     3301 ccaaaatgga atttggcaag atccgcgacg gttttgtcat tccattcaat atgccagcca
     3361 ttgttgaact gggcacggca acgggtttcg actttgagtt aattgatcag gctgggctgg
     3421 gtcacgatgc cctaacccag gcccgtaacc agttgcttgg tatggcggcg caacatcctg
     3481 ccagcttagt cagcgtgcgt cctaatggcc tggaagacac cgcgcagttt aaactggaag
     3541 ttgaccagga aaaggcgcag gcattaggtg tttcactttc tgacatcaat cagaccattt
     3601 caacggcgtt gggtgggact tacgttaacg acttcatcga ccgtggtcgc gtgaaaaagg
     3661 tgtatgttca ggcggatgcc aaattccgta tgctgccaga agatgtcgat aaactttatg
     3721 tccgcagcgc caacggcgaa atggtgccat tctcggcctt taccacttca cattgggtgt
     3781 atggctctcc gcgactggaa cgctacaacg gtctgccgtc aatggagatt cagggggaag
     3841 ccgcgccagg aaccagttcc ggcgatgcca tggcgttgat ggaaaacctt gcgtcaaaat
     3901 tacctgctgg cattggttat gactggacgg gtatgtcgta tcaggaacgc ttatcgggaa
     3961 accaggctcc cgctctggta gcaatttcct ttgtggttgt tttcctgtgc cttgctgcac
     4021 tctatgaaag ctggtcaatt cctgtctcgg ttatgttggt cgtgccgtta gggattgtcg
     4081 gcgtgctgct ggcggcgaca ctctttaatc aaaaaaatga cgtctacttt atggtgggct
     4141 tgctaacgac aattggcttg tcggccaaaa acgctatttt gattgttgag ttcgctaaag
     4201 atctcatgga gaaagagggt aaaggtgttg ttgaagcgac actaatggca gtacgtatgc
     4261 gtctgcgtcc tatcctgatg acctctctcg cctttattct cggcgtatta ccgctagcta
     4321 tcagtaacgg tgccggcagt ggcgcgcaga acgcagtggg tatcggggta atgggcggaa
     4381 tggtctctgc aacgttgctg gcaatcttct tcgtaccggt gttctttgtg gtgatccgcc
     4441 gttgctttaa aggataaatc agaaacataa aggcactttc gggtgccttt attattttca
     4501 gtgaaaccca taaaaattaa taagatattc ttccactcac tctttaaaaa ctttctatag
     4561 ttcccgctcc cttcactatt tttacaattc acataatttg tcattattca ttccgcaata
     4621 tttataacct ccggtgataa aatggcattc agctcgttaa taagagagtt aacttattaa
     4681 gcgttagcgt ttattactga ggtaacacca tgaaaagatt aattcctgtg gcattgctca
     4741 ccgcattgct ggcgggctgt gctcacgatt cgccctgcgt tccggtttat gacgatcagg
     4801 gtcgtctggt tcacaccaat acctgtatga aaggcacgac tcaggataac tgggaaacgg
     4861 ctggggctat tgccggcggg gcagcggctg ttgctggcct gacgatgggg atcatcgctc
     4921 tgtcgaaata actgaacagg gcgcggagtt tcgcgctctg tttaacgata cagtatcttt
     4981 acataagata aaatattgct atattctgct ggctaattct ctgctgattt agcatgttat
     5041 atcgcgttat ttcgctgctt tcgaaataac gcatcaattt aactttcaca cttcccacca
     5101 gaaatatact cacgctaaca atcattattc tgcttagtaa aacacgcgta aatattttgc
     5161 gccaaaatgt ggcgcatgtt tcattttcgc accattgcgg ggcgctgttt ttattatttc
     5221 ctgtctacac tcagcttatt gcgacgcgaa atcgggcgca aaagctggca ctacttttgc
     5281 ttataagagg atggccacag acaggtaaaa gacgtttccc aaacgtccta taacgataat
     5341 tttcgcctca caggaagcat tatgaaaaag atgatgatag ccacactggc tgccgccagc
     5401 gtgctgcttg ccgttgcaaa tcaggcgcat gctggagcga cgcttgatgc cgttcagaaa
     5461 aaaggttttg tgcaatgcgg gatcagtgat ggattacctg ggttctctta tgccgatgca
     5521 gacggtaagt tttcaggtat tgatgttgat gtttgtcgtg gtgttgccgc tgctgtattt
     5581 ggtgacgaca cgaaagtgaa atatacccca ctcacagcaa aagaacgctt cacggcttta
     5641 cagtcagggg aagtggattt gctctcccgt aatacgacct ggacttcatc tcgcgacgcc
     5701 gggatgggaa tggcatttac tggcgtcact tattacgacg gcattggctt cctgacgcac
     5761 gataaagcgg ggctaaaaag cgcgaaagaa ctggatggcg ctaccgtctg tattcaggcg
     5821 ggtactgata ccgaactcaa cgttgccgac tatttcaagg caaacaatat gaagtacaca
     5881 ccggtgactt tcgatcgctc tgacgaatca gcgaaggcac tggaatctgg tcgctgcgat
     5941 acgctggcct cggatcagtc gcaactgtat gccctgcgca tcaaattaag caacccagct
     6001 gaatggattg tcttaccgga ggttatctct aaagaaccgc ttgggccggt agttcgtcgt
     6061 ggcgatgatg aatggttctc gattgtacgc tggacgcttt tcgccatgct gaatgctgaa
     6121 gagatgggca tcaattccca gaacgtcgat gaaaaagcgg ctaatccagc aacgcctgat
     6181 atggcacatc tgctgggtaa agaaggcgat tacggcaagg atctgaagtt ggataataaa
     6241 tgggcctata acatcatcaa acaggtgggt aactactcgg aaattttcga gcgtaatgta
     6301 ggttcagaaa gcccgctgaa aattaaacgt gggcaaaata atctctggaa taacggcggt
     6361 attcagtacg cgccgcccgt gcgttaagtc ggtgtgaggt aacgagcgtc gctacggcgg
     6421 cgctctcttc agcgttatgc tttccgaggt ttctatgtct catcgccgct caaccgttaa
     6481 aggctcactc tcctttgcca accctacggt tcgtgcctgg ttattccaga tccttgccgt
     6541 tgttgctgtt gtcggcattg ttggttggtt atttcacaac actgtaacga atctcagtaa
     6601 tcgtggcatt acttcaggtt ttgcctttct ggatcgcggc gctggcttcg gtattgtcca
     6661 gcatttgatc gattaccagc agggcgacac ctacggacgc gtttttattg tcggcttact
     6721 caatacgcta ctggtttctg cattgtgtat tgtgttcgct tctgttttgg gcttctttat
     6781 cggtctggcg agactttcgg ataactggct gctacgaaag ctttccacaa tttatattga
     6841 gatctgaacc gccccggaaa tcctggagac taaactccct gagaaagagg taaacaggat
     6901 gactaaaaat a
//
LOCUS       DAZFJF010000197         6802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFJF010000197 DAZFJF010000000
VERSION     DAZFJF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.199"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..6017
                     /gene="efa1"
                     /locus_tag="ACHKAU_004635"
     CDS             <1..6017
                     /gene="efa1"
                     /locus_tag="ACHKAU_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO5533556.1"
                     /translation="LPLAYDYILNKKESSSIPVEILNKLSELPPHELLTPVLGQSVNP
                     LGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIHVRMREGTLNSQNLRQ
                     LLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPTIADATLSLLSVGLPG
                     TGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPVVSSLHFIAEHAKY
                     TLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMGLALLYLQTEDTAH
                     YSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQYQGNKYITNESLLH
                     KTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTDPNFGHADFLSPIDAL
                     KFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLLPENDAGLSTRIQHTT
                     TDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQIPSLRLNGDVLNDYL
                     SRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVSSQTRLQRQATRVKQQ
                     LAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIRDGETLHTTSVEVPEV
                     VSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHEDSTLAKINLAMDIKQ
                     LSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRTGGTMGKALSASARVL
                     ELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSISLGLTAASVAFPPLII
                     ATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHIVVASPERGLLDFSGN
                     KVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREKVGYANSISPYSSLAH
                     GYANSKWPQTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWIVWREAVPDSTSRHKR
                     PPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKDYKFILRGGSGGLAVQ
                     VGGAGYYDIDANPVAKENTLSFRGLPEEFPLAFDLSKQTQSVMLKTTDGEVPVMTITQ
                     KGINTLVGTTAGENWLTGNDKDNTFHTSSGGGTVISGGGNNRYIIPRDLKTPLTLTLS
                     NSSVSHEILLPETTLTELKPVAFELSLIYWAGNNIKVQLEDETQLNRFAGNFRGHTSD
                     GITLEAVSQENGIQLGVSSCDVQRWQAVYPEENNRPDAILDRLHDMGWSLAPEVRFRG
                     GETSASYDPLNRQLVYRLQERYSEFRLTGSRHYTTAVTGTPGSRYIITGPDTAQISPA
                     QIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYSLQLTISGIDESLPGT
                     TRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLENLMALNQVMTFLPRVS
                     GSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVNISDLSIPPYSRLYLI
                     FEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKPDNEAPSLILSEFRRF
                     TISSDKKFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLRLTFRDFFTEVMDTTD
                     REILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIVAEYARIQKEETSKIL
                     YQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGEPFPTFHPWYTDDLSGRYKSVPMARKA
                     DTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNLSILSEIPQNNNTVVH
                     SILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSYDSHSSGRVYSREEYM
                     MWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPGYIRSTVSKWSRGWLK
                     TGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAGADIADNAPGETRCTL
                     RPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDNLFN"
     gene            6189..6539
                     /locus_tag="ACHKAU_004636"
                     /pseudo
     CDS             6189..6539
                     /locus_tag="ACHKAU_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2012 a         1553 c         1564 g         1673 t
ORIGIN      
        1 aattaccact agcatatgat tacattttaa ataaaaaaga atcaagctct attcctgttg
       61 aaattttgaa caaactttca gagttaccac cacatgaact actcacacct gttcttggcc
      121 agagtgttaa tcctctgggc atgggctact catccgataa tggaaaaatc acagagcaag
      181 taatagtcag tggagctgat ggatttgata atcccatatc tggacttata tatacctatc
      241 ttgaagatct atataacatc catgtaagga tgcgagaagg tacactaaat tcacagaatc
      301 ttcgtcagct tctggaaaac tctgtttctt catgcttttt gactgagcaa agtattaata
      361 aattacttag tgaggcagaa aaaaggcctt atcagtcttt aacagaaata catcagcatc
      421 tcacaggatt accaactatt gccgatgcaa ccctttcatt actttctgtt ggattacctg
      481 gtacgggaaa actattgcgc agggagcagg actatgggcg tccaccagtt acagcaattc
      541 aggattccac atttgtactc ccttataatt tcaaaggtat tggttttaac gataacatta
      601 tatcctctgc acctgtagtc tcctcattac attttatcgc tgaacatgcg aaatatactt
      661 tgttgtcatg gcctgagttt tatcgtcatc atgcacagcg atggttcgaa atggctaaag
      721 gatatggaag ccagaatatt gattttcacc ctcagtctct attggtaacc caagaaggac
      781 gctgtatggg attagcctta ctttatttac agactgaaga tactgctcat tatagcattc
      841 tccaggaaaa cctaatgact gtgagtgcac ttcatcagac cagtaatcgc gataagttgc
      901 cactgtccaa agatgataat tccttaatga caagaactta tagtctgatt gaaatgctac
      961 agtatcaggg aaacaaatat attaccaacg aatcgctact acataagacc gcatggaacc
     1021 aagaaagaat aactttatta ttcaatgaaa aaggagttaa gcgagccctg ataagcacgc
     1081 ctaatcatac tctggttctg caacaactgg aggatattta ccggctcact gatccaaatt
     1141 ttgggcatgc agatttcctt tcacctatag atgctctgaa gtttattgag gctatgatac
     1201 aattaactcc aacacttcag gaatattatg gcctattaaa caaagacatt aataaacata
     1261 tacaagtaca ttatgccgaa tcagatatgg tctggaataa gcttctgcca gaaaatgatg
     1321 ctggactgag caccagaatt cagcacacca ccaccgaccg tctggcgaat ctggctgaac
     1381 cagtcgctgt tgcaggtatc tccctgccag taaaaacact ttatgatatc ggagccaccc
     1441 ttgacggtcg gcgcatcacc tctcctccaa catcggagca aatcccttct ctgcgtctca
     1501 acggtgatgt tctgaatgat tatctgtccc gcacagttct gactccagaa caggctgata
     1561 acataagaaa aatactgcac actcagggaa tacgcagcgg tacccgtccc atagatccgg
     1621 agatgattcg tgggacgcag gatgacctag tttcgtcaca gactcgtctg caaaggcaag
     1681 caacacgggt taaacagcaa ctcgccggcg tacttgatac tctgcaacag cacttccaga
     1741 acattccacg ttcatccggt cgtcatcttt ctgtagagaa tattgagctg gctgatatcg
     1801 gaagtgggcg tttcaacctt caaattcgag atggagaaac attgcatacc acttctgtgg
     1861 aagtaccgga agttgtgtcc cgttttcaga aactttctac catgctttca gccctgcctg
     1921 ccagtggaat catggatttc gacctcggca tgagtgtggt cggagtcgtc cagtatgccc
     1981 gcctgctaca gcaggggcac gaagacagta ccctagccaa gataaatcta gctatggata
     2041 tcaagcaact ttccgaagca actctcggca gcatgattca gattgccggg aataagtttc
     2101 tcaatacaga aggaatccag gggttcagac tggaaagcgc cgtcgctgaa ggtatgcgct
     2161 cagtagcaac ccgtaccgga ggcacaatgg ggaaagccct ttctgccagt gcccgtgttc
     2221 ttgaactgcc tgtactggaa acagttctgg ggacatggaa cctgtacaac agcgtcattc
     2281 agctccagca agctacatct tattctgaga caatggctgc ccgggtacag attgcgtttg
     2341 attccatttc tctgggatta actgccgctt cggtagcttt cccgccacta attattgcca
     2401 ctggccccat tgcggctatt ggtatgggag cttccagtat tgcacgtaat gtggcacgga
     2461 aagaagaacg gcatacacaa tggctggaat ataaaaaatt cctgactgat ggcagtaaac
     2521 acattgttgt ggcctctccg gaaagaggtc tgctggattt ctccggaaac aaagtttttg
     2581 gaaaaatggt gctggatctg cgtcagtctc ctcctctctt gcatggagaa agctctttta
     2641 acgctgaccg caaaatcggt catcgtccgg atctggggga ctggcaaatt cgtgagaagg
     2701 tggggtatgc caacagtatc agtccctact cttccctggc gcacggttac gccaacagta
     2761 aatggccaca aacaataccg aaaattccct cgggagaata tgacaccata attctgggct
     2821 acggtcacca gtatcaggcc aatacggaaa tagaatatct gtctaactgg attgtatggc
     2881 gggaagccgt accagacagt acttcccgcc acaaacgtcc tcctctggag gttcttaata
     2941 gtcagtgtac tgtgatagct ggagagcgta aaaccacagt acttcccctg agagtgctca
     3001 gcgatctgac accggaatgc acagaacagg ctatatcgtt aaaagattat aaattcatac
     3061 tgagaggggg aagcggtggg ctggctgttc aggtcggtgg cgcgggatat tatgatattg
     3121 atgcaaatcc tgtggcaaaa gaaaatacgc tctcttttcg cgggctaccg gaagagtttc
     3181 cgctcgcctt tgatttatca aaacaaacac agtcggtcat gctgaaaaca acggacggtg
     3241 aggtgccggt aatgaccatc acccagaagg gaataaacac cctggtcggt acaacggccg
     3301 gcgaaaactg gctaacgggt aacgataagg acaatacctt ccatacgagt tctggtggcg
     3361 gtactgtcat ctctggtgga gggaataacc gctatattat cccccgcgat ttaaaaactc
     3421 cgctgacact gacgctgtct aatagctcgg tctctcacga gatccttctg ccagaaacaa
     3481 ctctgacaga attaaaacct gtcgcctttg agctgagtct gatttactgg gccgggaaca
     3541 atataaaggt tcagctggag gatgagacac aactgaaccg ttttgccgga aacttcaggg
     3601 ggcataccag tgatggcatt actctggagg cggtttccca ggaaaatggt attcaactgg
     3661 gggtttcatc atgtgatgtt caacgctggc aggctgttta tccggaagaa aacaacagac
     3721 cagacgccat actggacagg ctgcatgata tgggatggag cctggccccc gaggtcaggt
     3781 tccggggagg agaaacatct gccagctatg accccctgaa ccgtcagctc gtttaccggc
     3841 ttcaggagcg ttactcggaa ttcaggctga ccggcagtcg ccattacacc acggctgtaa
     3901 ccggaacccc cggaagccgg tacattatta cggggccgga tacagcacag atatcaccgg
     3961 cacaaatcat actggccggt gataatgacc atccggaaac gattgatttg cttgaggcca
     4021 gtcctgttct ggttgaaggg aaaaaagaca aaaacagcgt gatattaacg attgctacga
     4081 ttcagtattc ccttcaactg acaatatccg ggatcgacga atcgctgccc gggacaaccc
     4141 gtgtggcaat tcagcctcag gatacccgtt tactgggtga cgtactccgg atcttaccag
     4201 ataatggtaa ctgggtgggg attttccgga gtggtcatac accaacggta aaccggctgg
     4261 aaaatttgat ggcactgaat caggtaatga cgttcctgcc ccgggtatcc ggaagtgcag
     4321 agcaggtatt atgcctcgaa aacctaggtg gcgtaaggaa aaaagtggag ggggagttac
     4381 tgtcagggaa gctgaaaggt gcgtggaaag ccgaaggtga acctactgtt ccggtaaata
     4441 tctcagatct aagtatccca ccctattcac gtctgtatct gatttttgaa gggaaaaata
     4501 atgtgttgct acgcagtaaa gtacatgcag ctccgttgaa aataacatcc gcaggagaga
     4561 tgcagttatc tgaaaggcag tggcaacagc aggaacatat tattgtcaag cccgacaacg
     4621 aagccccctc attaatactc agtgaatttc gtcgtttcac tatttcatcg gataaaaaat
     4681 tttctttaaa actgatgtgc catcagggga tggtccgcat cgaccgcaga tcattatcgg
     4741 tcagattgtt ctatctgcgt gaacagccag gtatcggcag tttacgtctg acgttcagag
     4801 attttttcac agaagtgatg gatacaacgg acagggaaat tctggagaaa gagctgagac
     4861 caattctgat aggagataca caccgcttta tcaacgctgc atacaaaaat catctgaata
     4921 tccagttagg agatggcgtt ctgaatctgg cagacattgt tgcggaatac gcccgtattc
     4981 aaaaggaaga aacatcaaaa atattgtatc aatatcaagg tgccatgaaa aaaaaaacag
     5041 atggaccatc tgtggtagaa gatgccatta tgaccactac tgtcacaaca gattcaggtg
     5101 aaccattccc taccttccac ccgtggtata cagatgattt atcagggcgt tataagagcg
     5161 tacctatggc aagaaaagca gatactttgt atcacctgac accaaaaggt gatctacaga
     5221 taatatatca ggtagctaca aaaatggtga atcaggcgat gattgtatcc ctgccaaact
     5281 accgacacga gtgggaaaaa tataatttaa gcatcttatc cgaaatccct cagaacaata
     5341 atactgttgt acattcaatc ctcagggtta atggccccac aatgcaggtg cgcacaattg
     5401 actacagagg aacggatgaa aacaatccca tagtatcttt ttcagataca accttcatca
     5461 atggtgaaca gatgttgagt tatgactcgc attcatcagg gcgagtctat tccagagaag
     5521 aatatatgat gtgggaattg cagcaacggg tatcagaagc ttccagtgcc cggacacagg
     5581 attactggct gatggatgca gcggtaagaa acggagaatg gaagatcaca ccagaattat
     5641 tacgtcacac accgggatat atccggagta cggtatcgaa atggtccaga ggatggctga
     5701 aaaccggcac aatactccag actccagaag acagaaatac ggatgtatac ctgactacca
     5761 tacagaacaa tgtatttagt cgtcaggggg gcggctacca agtgtattat cggattgatg
     5821 gaatggctgg tgcggatata gcggataatg caccagggga aacccgctgc accctcaggc
     5881 ccggaacatg ttttgaagtg acaagtgtgg atgaaaggca ttatgagtgg aatatcattt
     5941 atgtcacgct gaaaacctgt ggctggagcc gaaatggcca aagcaaaacg ccgaatggtg
     6001 acaacctttt taactaatta aaatctctgt taaagatgta taacccggca tctatgtgct
     6061 caccagcata tagatgcaat gtgattcccc tgatggtcgt atcagagatg gtatgcgagc
     6121 ctgtacatag atttgtgtaa ttgcctgatt ttgatatgtt caatccaaca tcaaaagcag
     6181 gttaatttat ggacgaaaaa cagttgcagg ctctggctaa cgaacaggcc aaaaatctca
     6241 aaacccctga agatctcagc cagttcgatc gcctgctgaa gaaaatcagc gttgaggcag
     6301 ctctcaacgc tgaaatgtcc caccatctgg gctacgataa aaaccagcct aaaccgggtg
     6361 ccaactcccg caatggctat tccacaaaga ccgttatcac cggtgatggc cccctggaac
     6421 tacgcactcc gcgcgatcgt gatggctctt ttgaaccgta acttgtgaag aaaaaccaga
     6481 cccggatcac cgggatggat aaccagatcc tgtcgttgta cgccaaaggc ctgacaaccg
     6541 taagcgtaca gcctgaaccg tctggtcaga atctgacgaa ttagagtaag ctggacatcc
     6601 ccagatatat tatggctgcc agcgtcatat cggcataaat agagatatcc cttaacgcta
     6661 accagaaaaa aatacctata agagcctgta catagagttg tgtcgttgcc tgatgttgat
     6721 atgtcaaatc caacatcaaa tagcggtaaa tttatggata aaaaattaca ggctctggtt
     6781 aacgaaacac ccccctcttg ct
//
LOCUS       DAZFJF010000198         6794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFJF010000198 DAZFJF010000000
VERSION     DAZFJF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.200"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            286..780
                     /locus_tag="ACHKAU_004637"
     CDS             286..780
                     /locus_tag="ACHKAU_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533557.1"
                     /translation="MDVLSGITAAKQAYDLLKTIKETRDDAVIAKAIGDLHQRITDLQ
                     MLNAELSGLYQAEKEIAMKLRDENRKIKMFVVQAENYELHTTEGGSVVYRPKSHSDPS
                     IQQHNLCAHCFGEHKISILQPSTVTIKSNGFFVHSCPRCKNEYRMYRAPDPKPVYVPP
                     LTNY"
     gene            complement(811..1173)
                     /locus_tag="ACHKAU_004638"
     CDS             complement(811..1173)
                     /locus_tag="ACHKAU_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HFO5533558.1"
                     /translation="MGQEEKYELKKLIGEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVKTEGDIPLTK"
     gene            complement(1176..1448)
                     /locus_tag="ACHKAU_004639"
     CDS             complement(1176..1448)
                     /locus_tag="ACHKAU_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533559.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            complement(1882..2178)
                     /locus_tag="ACHKAU_004640"
     CDS             complement(1882..2178)
                     /locus_tag="ACHKAU_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533560.1"
                     /translation="MEFLIVFVVVLVIILFVLLSISKKLSQMIEHSSNRAKEEEHIID
                     IKEILSDIKITLDEIKYTTDLIEQYKIPTPNERKAIDQYRIDLEIDEMLSKRKD"
     gene            complement(2219..2926)
                     /locus_tag="ACHKAU_004641"
     CDS             complement(2219..2926)
                     /locus_tag="ACHKAU_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5533561.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRDSSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            3038..3262
                     /locus_tag="ACHKAU_004642"
     CDS             3038..3262
                     /locus_tag="ACHKAU_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5533562.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3372..3650
                     /locus_tag="ACHKAU_004643"
     CDS             3372..3650
                     /locus_tag="ACHKAU_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO5533563.1"
                     /translation="MELTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            3686..3847
                     /locus_tag="ACHKAU_004644"
     CDS             3686..3847
                     /locus_tag="ACHKAU_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533564.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            3834..4655
                     /locus_tag="ACHKAU_004645"
     CDS             3834..4655
                     /locus_tag="ACHKAU_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5533565.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEIIDSLLMADLT
                     VRQMKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAESANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            4652..6028
                     /locus_tag="ACHKAU_004646"
     CDS             4652..6028
                     /locus_tag="ACHKAU_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5533566.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDTSSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERNKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGPLGTVYQRFCNGHFVACDQDEARQICTASNAPASRGR
                     RYAQGADV"
     gene            6115..6330
                     /locus_tag="ACHKAU_004647"
     CDS             6115..6330
                     /locus_tag="ACHKAU_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533567.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPIDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
BASE COUNT         1831 a         1480 c         1624 g         1859 t
ORIGIN      
        1 ctgtcgcctg gaatctccag aaaacaggca ttccatcatg ttcagtttct gattcaggaa
       61 aaggtacgct ccatgatttt gtcatatctc acctcaaata agtggtttgc tgtctaattt
      121 cattttctgg cgaccaacac aagtcacacc catttcactg cgtggcttgc ggtagtaaag
      181 attgtgcctg tcttttaacc acatcaggct cggtggttct cgtgtacccc tacagcgaga
      241 aaaatagtaa aatcctctta cccctacagt aagagagtaa tttatatgga tgtgttatct
      301 ggtataactg ccgcgaaaca ggcttatgat ctgctgaaga caataaaaga aaccagagac
      361 gatgctgtta tcgctaaagc tattggtgat ctacaccaaa gaataactga cttacagatg
      421 cttaatgcgg agctctctgg cctttatcag gccgagaagg agatcgcaat gaagcttcga
      481 gatgaaaata gaaaaatcaa gatgtttgtt gtgcaagctg agaattatga acttcataca
      541 actgaaggtg gttctgtcgt gtatcgacct aagagtcatt ccgatccttc tattcagcag
      601 cataaccttt gtgcacattg cttcggcgaa cataaaatat cgatacttca accaagcacg
      661 gttactataa aatctaatgg gttctttgtg cattcatgtc ctcgctgtaa gaatgaatac
      721 cggatgtata gagcgccaga ccctaagcct gtatatgtac cgccgctgac aaattattaa
      781 ttttattcct gaaatggcct cttcaccccc ttactttgtc agggggatat ctccttcagt
      841 tttgaccatt cgccttaata ctttccttaa gtcgatgtaa agttgaaggt ctccatttgc
      901 tgcggcatca gccatttttt gcctgacaag cagtaatgtt tcatacggct caataagaat
      961 atcgtcatga gtaattaggt gaagcgttgc cgcatcaact attcctagag ctgcgccaag
     1021 tatcaaaaat tccctgctat ttttgtcgca tgaggagata agcgtattta gcgcatacct
     1081 aatattcttt atagctgttg ttaatgctgc aatttcttct atggcgtctt ctccaatgag
     1141 ctttttaagc tcatattttt cttcctgacc cataattacc tcgccgtcag ttgttttgat
     1201 ttccggtagc ctgccgcgta aatggctacg tttggcaggc aaatacttcc actgcattca
     1261 tctgccttct tgcagcgaag gcttccgagt gatgctgctt tatctgctct gacgcaacca
     1321 gagagcttta gcgcaatttt tcgtgccaat cgctgttctt gcattgcctg ttcacgttga
     1381 gcctgtctgc gtgctctgcg gcgatttctg gcgttatcgt cagccagata tgtaatgact
     1441 actgtcatgt tgacctccga tgattgactt tggcggtgac gcgccgcgtg cttatcttcc
     1501 ggttgccgtc ttgcagctgc acttcacgtc accccaaagc caactactct ttggttcccg
     1561 catttcggcg ggacaatccc atcaatgtta aagagcctgc caatctgttc cgtttggctt
     1621 ccagcgtcct gctgatggct taaagataac ttaggttata ggtgtggtca ataacctaat
     1681 ttatatttta tggtgaatag gttataagtg atggataaca aaggtatttt atttttgtaa
     1741 atgttgctga ttgattggtg tttgagggct tgcgtgcggg gtgaaggtgt tacctttggc
     1801 ttgatgcttg tctatgatga ggatggttga ttgggtggtg aacggcagga aaagaaaacc
     1861 cggcgctgag gccgggtgtt tttagtcttt tcttttgctt agcatttcgt cgatttctaa
     1921 gtcaatacga tattgatcta ttgcttttct ctcgtttggg gttggtattt tatactgttc
     1981 aattagatct gttgtgtatt ttatttcatc taatgtgatt tttatatcag agagaatctc
     2041 ttttatatca attatatgct cttcttcttt tgcacgatta gagctatgtt caatcatttg
     2101 agatagcttt ttactaatgc ttagcaaaac aaaaagaatg atgaccaaaa caacaacaaa
     2161 aactatcaga aattccatta tccctccgca cttccgtaag tcttcttctg attatcggtt
     2221 accaactatg agacgaccag aatactctgc caataatcct tacggtttca tgaaattcat
     2281 ctctatccat tacttcatcc gggtactctt cgcgatttat tgatctgatt atcaccgatg
     2341 taggggtggc gattaatgtt tttactcgta acaaatcaga ctggcaaata gcgtaggttt
     2401 taccatctct gattgtggta tcttgcgtgt taacaccaac aacatcgcca tcgtgaagcg
     2461 ttggttccat gctttgccct acaaccctaa ctagcttggc tgatctttca gatactccca
     2521 tctttttcag atagtgcttt ctgaaaacca aagagaactc cgatgattcc tctagctcgc
     2581 agctaccgct tccagctgaa agcgaaacgt taagaagagg caacgcaaca aactcgtcat
     2641 cgtttctttt aatgtcttcc cataccacag cttttaaaga tgaatcacgg acattggatg
     2701 gttcttcatg tgcaccatcc ctcatttcac caataccaga actaagccat tcagggcgca
     2761 cttttaaagc attggctaat tcaaccatct tgcgagatcc gtttgtttta ccggacgaca
     2821 tcttctgtat ggctggctga gatattccaa ccatgtcagc aagctgtgat tgtgataccc
     2881 ctgctgagct catggctgca tttagtcttt ctgcgaatgt tttcataccc acaaatctat
     2941 aactacggtt atccaaagta aaataacaaa ggttattgct attttttata acttgagtta
     3001 tctttggtta taagtaatga ccacaagagg tatgctcatg aatttagtaa ttcaacgagc
     3061 cttgaaaatt gtcggtagcc aaaagcgcct tgccgacaag tgtggtgtaa cgcagccagc
     3121 agtacacaaa tggctgaaag gcgggttggt ctctccagag aaagttaccg ccatcgttaa
     3181 cgccactgga gggcagatca aggcttacga aattcgcccc gatttgccac acctgtttcc
     3241 aaaaccgaat caggcagcat aagtaacacc gctctttaac agtcatggtc ctcattcccg
     3301 ccgaaatgcg ggaatacaac gcgcataagt tgatgcgcat aacttcttat ttgttaagga
     3361 aatacttaca tatggaactt acaagtactc gcaagaaagc gaatgcaatt acaagcaaca
     3421 tcctgaatcg aattgctgta cgtggtcagc gaaaggttgc tgatgcatta gggatcaatg
     3481 aatcgcaaat ttcgcgatgg aaagacagct ttatcccaaa gatggccatg cttctggctg
     3541 tgctggagtg gggtgttgaa gacgaggaat tagcggagct ggcaaagaaa gtagccatgg
     3601 tgctgacaaa agaaaagcct caagactgcg gcaacagttt tgaggcctga ttgtagaaag
     3661 actgtatcaa tccacaggag taattatgac aaaacgtcgt aagaaatacc aggaaaaaga
     3721 agagattcga caccctgatt cacctgaggg attagtggta gccgcagcaa ataacagggc
     3781 gttcgcagag cgccttgttg gtgtttacag actagccaaa gcaggagtga aacatgggcg
     3841 tcgttaagtt agctgattac aggcctcaac tggaggtcgt ggagcatcgc gtggcagata
     3901 ccgaagatgg tttcatgcgc gttgctaacg agattatcga cagtctgctg atggctgatt
     3961 taaccgtccg gcagatgaag gtgatgctcg ctatcatgcg caagacatac ggattcaata
     4021 agccgatgga tcgactcaca aacacgcaga tagcagccat gacaggtatt catcacactc
     4081 atgtttgcgc tgccaagcgc cagcttattg agcgtaaatt cctcattgct gatggcgtga
     4141 aaatcggagt gaacaaggtg gtttctcagt ggattagcca ggacagctta acattagcta
     4201 aaacagctaa taaaacatta gccgagtcgg ctaatgggta taagccaagt cagctaaaca
     4261 caaaagacaa tatacaaaag acaataaata caaatacccc cttaccccct aacgggggcg
     4321 gcgatgggca ggttaaacct gaacgtcgca aggcagaacg aatcgactac gaatccttcc
     4381 tgaacgccta caacaccgaa gtcggtgaca gacttccaca cgctgttgcg gtcaacgaga
     4441 aacgaaaacg ccgcctgaag aaaatcatcc cgcaactgaa aacgccaaac gtggacggtt
     4501 tcagagcgta tgtcagggcg tttgtgcatc aggccaagcc gttttacttc ggagacaacg
     4561 acacgggctg gacggcagat tttgattacc tgctgaggga agactcgtta acgggagttc
     4621 gggaagggaa gtttgcagac agggggattg catgagacag gatatcgaag cgagcgttat
     4681 cggtggcttg ctgattggtg gattaacacc aaccgccagc gacgttctgg caacgctgga
     4741 gccggaagcg ttttcaattc cgctctaccg gaaagccttc gaagttatcc gtaagcaggc
     4801 gagaaacaga aacctaatcg acgcgctgat ggttgccgag gcgtgcggag aggagcattt
     4861 cacgtcaatc ctgatgacca gcaaaaactg cccgagtgcc gcaaacctga agggatatgc
     4921 cggaatggtc gcggataact atcaccgccg tctggtgctg gaaatcatgg acgaaatgcg
     4981 tgaaccaatt cagagcggaa ccatcgatac atcgagtcag gcgatggacg agcttgtaaa
     5041 gcgtctctca gccatcagaa agccccgtga cgaggttaaa cctgtacggt taggggaaat
     5101 catcactgac tacactgaca cgcttgacag gcgtctgagg aacggagaag agtcagatac
     5161 cctgaagacc ggaatcgaag aacttgacgc catcaccgga gggatgaacg cggaagacct
     5221 ggtgattatc gccgctcgtc ctggtatggg gaaaaccgaa ctggcgctga agattgccga
     5281 aggtgttgca agccgcgtta ttcctggttc tgacgtccgg cgcggagtgt tgattttctc
     5341 gatggaaatg agcgcattgc agattgcaga gcggagcatt gccaacgccg ggaggatgtc
     5401 ggttagcgta ctgcgaaatc ctgcatcgat ggatgacgaa ggctgggcgc gtgttgctaa
     5461 cggcatgagt cagcttgcag atttggatgt atgggtagtc gatgcctcgc ggttatcggt
     5521 agaagaaatt cgctcaatcg cagagcggaa caaacaggaa aatccaaacc tgtcactcat
     5581 catggcggat tatcttggcc tgattgagaa gccgaaagca gaccgcaacg acctcgcaat
     5641 tgctcacatc tcaggaagcc tgaaggcgat ggcgaaagac ctgaaaacgc ctgttatctc
     5701 cctaagtcag ctttcgcgcg atgttgagaa gcgaccaaac aaacgcccga caaacgcaga
     5761 tttgcgtgat tcaggaagca ttgagcagga cgcagactca atcatcatgc tctatcggga
     5821 agcggtatat gacgagaaca gtagcgccgc accatttgct gaaatcattg tgacgaaaaa
     5881 ccggtttggc ccacttggta cggtttacca gcggttctgt aacggacact ttgttgcatg
     5941 tgaccaggat gaagccagac agatttgcac agcatcaaat gcacctgctt cgcgtggcag
     6001 acgatatgca caaggggctg acgtatgacc atctacatca ctgagctaat aacaggcctg
     6061 ctggtaatcg caggcctttt tatttggggg agagggaatt ggaggcttta agaaatgagt
     6121 acgatagctg agcttgtcag ggctaatttt cgtgaagagt tggtgcgttg gtatcggtat
     6181 cgttcatcgt ccagtttgcc gattgatgag ttgtatgagc attcacctgc tgcacgacgc
     6241 tatccgcgtg accgtgtcct tcgacggttg ttcaaactca acaatgagtt tcagcgcaac
     6301 agaataatcc ggagtctgga tttaaagtga aggagtgagc atgagtcgaa gaagtagctt
     6361 tttggggttt gtaatattcc tgtcctgcac tggttacatc gtaatctggt caatttcgaa
     6421 cattgaccgt ggcggggaat atctcattgt aatgttcttt cctttgtttc ttgggtggta
     6481 cgccgcaagg ttgctggaag aatggggtta caggcataaa aaataaagga gtgttcagtg
     6541 aagcaaaccc ttttttattg gattaagagc gaggggtaag taccgatggt aaatgcattt
     6601 atttgtagtt tatttcttgt cgcgattttt catggattcc ttctgatgat gagttttgtt
     6661 ctctggaata atggatatcg catattggga gtaggttttg ttttacggtt ttcagttgtc
     6721 tgcgcgttgc taccgataat tatggcgact atcaaatatt attggtaacc caaaaaatca
     6781 tcgatggaga gtga
//
LOCUS       DAZFJF010000199         6736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFJF010000199 DAZFJF010000000
VERSION     DAZFJF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.201"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(194..1123)
                     /gene="fdhE"
                     /locus_tag="ACHKAU_004648"
     CDS             complement(194..1123)
                     /gene="fdhE"
                     /locus_tag="ACHKAU_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO5533568.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1120..1755)
                     /gene="fdoI"
                     /locus_tag="ACHKAU_004649"
     CDS             complement(1120..1755)
                     /gene="fdoI"
                     /locus_tag="ACHKAU_004649"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5533569.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1752..2654)
                     /gene="fdoH"
                     /locus_tag="ACHKAU_004650"
     CDS             complement(1752..2654)
                     /gene="fdoH"
                     /locus_tag="ACHKAU_004650"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO5533570.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2667..5717)
                     /gene="fdnG"
                     /locus_tag="ACHKAU_004651"
     CDS             complement(2667..5717)
                     /gene="fdnG"
                     /locus_tag="ACHKAU_004651"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5130..5132),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5533571.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5911..>6736
                     /gene="fdhD"
                     /locus_tag="ACHKAU_004652"
     CDS             5911..>6736
                     /gene="fdhD"
                     /locus_tag="ACHKAU_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO5533572.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRL"
BASE COUNT         1466 a         1833 c         1831 g         1606 t
ORIGIN      
        1 agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt
       61 tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa
      121 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
      181 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
      241 catagccttc ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg
      301 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
      361 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
      421 tctgctcgca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc
      481 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
      541 aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct ttgccgggga
      601 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
      661 cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
      721 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
      781 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
      841 gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca
      901 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
      961 gcaggtaatc acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
     1021 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     1081 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     1141 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     1201 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     1261 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     1321 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     1381 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     1441 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     1501 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     1561 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     1621 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat
     1681 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcaggatggt
     1741 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     1801 tccgcacggt tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca
     1861 aagccaacag ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     1921 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     1981 attacgtgtg taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg
     2041 gttttcagct cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     2101 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     2161 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     2221 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     2281 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga
     2341 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     2401 acttccgaga agcgcattac cgtccacgat ttggcgctta aatcattggg gttgtcgtac
     2461 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     2521 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     2581 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga
     2641 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     2701 aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag
     2761 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     2821 ttgccgttcg ctttcagtgt acgaatacgt ttggtcacca ccgctttggc cttgatatag
     2881 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     2941 gactccccga tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg
     3001 gtccagtagt ggaaatgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     3061 gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt cgagataacg
     3121 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     3181 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     3241 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     3301 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     3361 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     3421 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc
     3481 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     3541 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     3601 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     3661 tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc gccaccctgt
     3721 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc gtcagtcagc
     3781 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg
     3841 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     3901 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     3961 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     4021 ttgttcggga atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgcct
     4081 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     4141 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     4201 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     4261 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     4321 cctggcagac tctgcgacag tagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     4381 cgcagggcgt taacgccgcc gccggccatc cccatattgc cgagcagcag ctggatcatc
     4441 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     4501 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     4561 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     4621 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     4681 ccgttttcgt ccagttcata agtccaggag gatttatcgt acttgcgttt ttccgcgtcg
     4741 tagccggtga acaggccatc ttcaaagccg taatcctcac gcactatcag gctggcgttg
     4801 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     4861 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtc agctaccgcc
     4921 gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     4981 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     5041 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     5101 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     5161 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     5221 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     5281 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     5341 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     5401 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     5461 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     5521 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     5581 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagcgctac gctgggtgca
     5641 aaacccagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     5701 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     5761 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     5821 cagaattggc tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     5881 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcaaaa agaaatcgaa
     5941 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     6001 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     6061 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     6121 ctttccgagg ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     6181 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     6241 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     6301 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     6361 gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact
     6421 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt
     6481 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     6541 caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     6601 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     6661 gagcgttgta atctgacgct ggtgggtttt tgtaagccgg gtcgggcaac ggtttatacc
     6721 catccgcagc gtttga
//
LOCUS       DAZFJF010000200         6715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFJF010000200 DAZFJF010000000
VERSION     DAZFJF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.202"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..278
                     /locus_tag="ACHKAU_004653"
     CDS             <1..278
                     /locus_tag="ACHKAU_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFO5533573.1"
                     /translation="KKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDS
                     EGVDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
     gene            complement(506..664)
                     /locus_tag="ACHKAU_004654"
     CDS             complement(506..664)
                     /locus_tag="ACHKAU_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5533574.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEI
                     LRVLEVVA"
     gene            complement(1324..1515)
                     /locus_tag="ACHKAU_004655"
     CDS             complement(1324..1515)
                     /locus_tag="ACHKAU_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO5533575.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGEDL"
     gene            1580..1903
                     /locus_tag="ACHKAU_004656"
     CDS             1580..1903
                     /locus_tag="ACHKAU_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO5533576.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQG
                     ARDDC"
     gene            2112..2543
                     /locus_tag="ACHKAU_004657"
                     /pseudo
     CDS             2112..2543
                     /locus_tag="ACHKAU_004657"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2581..3099
                     /locus_tag="ACHKAU_004658"
     CDS             2581..3099
                     /locus_tag="ACHKAU_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO5533577.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSN
                     ETKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVT
                     HYQGKDWIQLTE"
     gene            3106..3339
                     /locus_tag="ACHKAU_004659"
     CDS             3106..3339
                     /locus_tag="ACHKAU_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO5533578.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENWR"
     gene            complement(3714..3920)
                     /locus_tag="ACHKAU_004660"
     CDS             complement(3714..3920)
                     /locus_tag="ACHKAU_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533579.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQ
                     SGDGKLMATYRRNKKAIDKLDAEG"
     gene            complement(3913..4296)
                     /locus_tag="ACHKAU_004661"
     CDS             complement(3913..4296)
                     /locus_tag="ACHKAU_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533580.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKEL
                     ISKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINH
                     KISDTEDAVLSVGKIINLIEDSQHG"
     gene            complement(4687..5748)
                     /locus_tag="ACHKAU_004662"
     CDS             complement(4687..5748)
                     /locus_tag="ACHKAU_004662"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="HFO5533581.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMN
                     PIYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVI
                     KGNNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHE
                     IRKIFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKS
                     PILDQNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNI
                     NRIYTEMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPE
                     RRFDQSVYYTASHLFDEIK"
     gene            5836..>6715
                     /locus_tag="ACHKAU_004663"
     CDS             5836..>6715
                     /locus_tag="ACHKAU_004663"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO5533582.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSH
                     PIKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKR
                     IKPKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGK
                     LDVRDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTV
                     RGVNRPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGN
                     AVPVELAKYVAVTIMEY"
BASE COUNT         1768 a         1310 c         1752 g         1885 t
ORIGIN      
        1 aaaaaaaaca agtaaaaggt atttatgata aagaaggctt tagggcgtgg ttgctgaatg
       61 aaaagaaact aacaaaaaga acctctagcg atattatatc tagatgttgc agaggtgttt
      121 cattttttga ttcagagggg gtggattttt ataactgtga aatagatgaa attattatga
      181 aactggaaag gcttgaatcg tttgtcaggc ttggggttag cctgaagtct cagttaagaa
      241 gggcttttaa attatattac gaatattgtc gaaggtaata aatacgaatt tatgtctctc
      301 aggggagggg gaggggataa aaagatcaaa ggatcctctt gtgatccttt ttttctgcgc
      361 gtaatctgct gcctgtaaac aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa
      421 tctagagcta ccaactcttt cttccgaagg taactggctt ggtggagcgc agataccaaa
      481 tactgtcctt ctagtgtagc cgcagttagg ccaccacttc aagaactctt aatatctcaa
      541 tccaccttgt ccagttacca gtggctgctg ccagtggcgt taagtcgtgt ctttccgggt
      601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccaa cagcgtgagc
      721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tctgtgatgc tcgtcagggg
      901 ggcggagcct atggaaaaac gccagcggcg cggccttttg ttgtcctttg cctttttgct
      961 cacatgttct ttccggcctt atcccctgat tctgtggata accgtattac cgcctttgag
     1021 tgagccgact ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     1081 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1141 atatggtgct cttttcttat aatccgcact gatgccacat agttaagcca gtatacactc
     1201 cgctatcgct acgtgactgg ttcagggctt cgccccgaaa ccccctgacg cgccctgacg
     1261 ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg accgtctccg ggagctgcat
     1321 gtgtcagagg tcttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     1381 ggcgtggtcg tgaagagatt cacaaatatc ggcctgttca tctgcgtcca gttcgttgag
     1441 tttctccagc agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     1501 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     1561 accgatgaaa cgcgagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     1621 actggaacgc tgtgagggta aacaactggc ggtatggatg cggcgggtct gcctgggtga
     1681 gccggttgcc cgttccggga agctgccgac actggcaccg ccgttactgc gtcagctggc
     1741 cgccatcggg aataacctga atcagacagc gaggaaggta aacagcgggc agtggtcttc
     1801 cggtgaccgg gttcaggtgg tggccgcact gatggccatc gagggagagt tgcgcagctt
     1861 acggcaggtc gtcagggagc agggagcgcg ggatgattgt taaatttcat gccaggggaa
     1921 aaggcggtgg cagtggtccg gttgattacc tgctgggaag ggagcgtaac cgggaagggg
     1981 caacggtgct tcagggtaat ccggaagaag tcagggaact catcgatgcc acgccatttg
     2041 cgaagaaata cacgtccggt gttctgtcgt tcgcggagaa ggaactgccg ccgggaggac
     2101 gtgaaaaagt gatggagagc tttgagcgcg tactgatgcc cggtctcgaa aaaaatcagt
     2161 acagcattct gtgggtggag caccaggaca aggggcgtct tgagctgaat tttgtcattc
     2221 cgaacatgga gctacagacc ggaaaacgcc tccagccgta ctacgaccga gcagacaggc
     2281 cgagaattga tgcctggcag acactggtga atcaccatta cgggctgcat gaccccaacg
     2341 ccccggagaa ccgccggata ctgactctct ctgataacct gccggaaacg aaacaggcgc
     2401 ttgctgagag cgtcacacgc gggatagacg ctctttatca tgtaggggag ataaaaggcc
     2461 gtcaggatgt gattcaggcg ctcacagagg cggggcttga agtggtcagg gtgacgcgaa
     2521 gcagcatcag cattgcagac ccgaacggcg ggaagaatat caggctgaaa ggagcgtttt
     2581 atgagcaatc ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa
     2641 tctacagaga aaatgctgaa cgacgagttc aggaagctcg gcgagtctgt cagcgaggct
     2701 gtgacctcaa acgagacgaa aatcagagac gctatagccc tgttcacagc ctcgacagag
     2761 aaatcgctgg aaaaacacag ggaaggggtg aaagaggcga tgatgcagca caggaaggac
     2821 gtgttaaagc tggcagggaa tacgggcatg atgttactgg ggatagtctt tctcctgttt
     2881 accgcgagtg gcgggacgct ctggtatctt ggcgggagga tacaggcgaa cctggaagaa
     2941 atcaggaagc aggaagagac attgcagaaa ctgaacgcga agacatgggg cgtggagttt
     3001 gtgcaggacg ggagaaggaa atttctggtc attccgcaag ggatgtcagc agaggtgacg
     3061 cactatcagg ggaaggactg gatacaactg acggagtgat acggaatgac agagctggaa
     3121 acacatttgc tgaacgcctt agagcagctg caacaggact atatgcagcg gctgaacgaa
     3181 tgggagagcg ccttcgtgga attgcagaag atgttttcgc ttacgcaacg ggacaacgcg
     3241 atgctgaacg agcgggtcat gcagttgagt cagcaggtgc agcacttgag cgagcggaca
     3301 gaacgcttga gccagttata cagcgagaac tggagataag agaggaacgg ctgatacagg
     3361 agcgcgaaca tgcgttatcc ctggaacggg agcgtcagcc ggaaatacag gaacgcatac
     3421 tggatggtcc ttcgttgtcg ctgtgatggc gaaactataa aaaatggcag gttcagtgga
     3481 ttttgacgga ctaatgtagt ctgcaccatc tggttgcata ggtattcatg cggttaaaat
     3541 ttatcaggcg cgatcgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
     3601 gccgtgatcg caccgaacgc gttttagcgg tgtgcgtaac gggagttatg gtaaatacaa
     3661 tatgcagcca gtcttagaga cgaaaagtcc gcccagaaga ctgacttttc agatcaacct
     3721 tcagcatcta acttgtctat cgctttttta ttgcgccgat aggtagccat tagtttacca
     3781 tcgcctgatt gtaagtcacg acctgcttgc tcagcatagg tatcgtttac agtcttagcg
     3841 ccttttttat aggccttttt aacatcatca aatccggctt ggatgacttc tttcggcttt
     3901 tcttgaggta atttagccat gctgtgaatc ctctataagg ttgattattt ttccaacaga
     3961 taagacggca tcttctgtgt cgctgatttt atgatttatc caaccgtcct ctatctttaa
     4021 tttgttcact actcctaatg taaaaacaat ctttttttca ggaaagaaaa ttgcaaaggt
     4081 tctacttttc tctttccaat cagagggcgg cgtcgaacgg aacgctacat tcctcgattt
     4141 aagtatcgct ttgattttac taattaattc ttttttttgt ttattgctga gatttttgac
     4201 gaaccctttg ctgagattgt ctgtgtaatc atcaaaagaa atatcaactg gtttgttttt
     4261 ttgtttttta tcagatagat gaacaatgtt tgacactgga tatcctgctt tttgatgaat
     4321 gaatactcag aagagtagcg gaactgtctt gttagtcaca gaggtagagt atcaattcta
     4381 cgatagatgg gttcgaagat ccggctgaga ccgttcagta cgcggtcgag atgttatgtt
     4441 caatgtaatg accgtcgaaa ttgcctttac gggcatgaag caaccaggag caatcattgg
     4501 caaaggtaac tagagttcaa tgcatggtaa tcatttttga gcttgtgatg catagagttg
     4561 gttgctgttc cattgcgtct caaactccgt caagatgctc gccgtaaaag cgagcatgtc
     4621 tagtatgcct gttccgctac tgatttttga tgggcgaatt cagggctggc gagccaccaa
     4681 gaaaaattac tttatttcat caaacaggtg agatgcagta taataaacgc tttgatcaaa
     4741 gcgtctctct gggcttaatt tattttgccc gccatttaaa attatttctt ttaatactga
     4801 tggtgggagg atgggttttg ttcttcttgg gtgatccata taagcttttg ttatttttcc
     4861 gtttagcgag aactctttat cactttgata tccaaagttc atctcagtat aaattctatt
     4921 gatgtttgtc ttgtttttgc tcgcaatttc atacaccatt ctggcgagtc tggctgatga
     4981 tctcattagt ctgctggcag atgaattgtt atatgaggat ctatcaataa aggttcgaca
     5041 caaagcaaaa tcactccaga tgaatatgtc aaaggcattt tgatctaata ttgggctctt
     5101 gccttttgtt ttccataatg gttgcattaa aaaaggtttc tggtaatcaa gaaaattttc
     5161 ttggaaagtg tttaggcaat ttaatatatt ttctttcttt gaaagaactt cagtttcatt
     5221 cccccagtgc tgtatgcttt cgcatgtgga ttcaaatatt tttcttattt catggaattt
     5281 atcgctgcaa gagtgcatta tgccaagggc acaataagaa gtgcttgcag gtcttattac
     5341 aatctctgaa ctccattcgc tctcgttttc tttatatgtc gtgctatctg gaagtacggt
     5401 taattttatt tctagtgctt ttaaaaaact attgtttcct tttataacta gatctattcc
     5461 ttttatgtca tcataagcat actgttggta tggttcatat tttttctcaa attgaaattc
     5521 aagatcacta ttttttaatg ttgtgttaaa aacgaagtca aaagatactt ctttggcatg
     5581 cacttttaaa tccttatcta aataaagata aatcggattc attttgttgt ctctcatata
     5641 acatgctaga gaggtgggaa aagaactgtt aaattggttc tttccccaca gatcgctctc
     5701 tttcctgttg gtgtttttta aaccataaag agattctatt ttgctcactt tttgctcctg
     5761 acgtcaattt attgacgtct atggtgtgtt tctatatcat gatgtggagt gatatgataa
     5821 tgagtttttt atgttatgag aataattgat ctttttagtg ggtgtggggg gttgtcccta
     5881 ggctttttga agggggggtt tgatgttgta ggtgcttatg atttctggga tcctgctatt
     5941 gaatgttaca gagataattt tagccatccg ataaaaaaac tagatttgag caatgttgat
     6001 gatgtggtga gggagcttaa agatatagac ttcgatatga ttattggagg gcctccttgc
     6061 caagacttca gtcatgcagg tttaagaatt gaaggtgcta gagctaactt aactcgctca
     6121 ttttcagaaa ttataaaaag aataaagcca aagtggtttg tgatggaaaa tgtagatcgg
     6181 gcattaagaa gtggtgccta tttagaagct cgaggaatct ttaaagaatc agggtatggg
     6241 ttaacagaaa ttgttttgga tgccagtaag tgtggtgttc cgcaaaaaag aaaaagatta
     6301 tttgtaattg gaaagcttga cgtgagggat ggttttatcc ttaacgaagt gatgtgcggg
     6361 atatcgaaag atagtatgac agttagaaat tatcttggtg attcattggg gattgagtat
     6421 tattacagac atccgagaaa ttataacaga agggctattt tctctattga tgaacctgcg
     6481 ccaacagtaa gaggggttaa tagaccgata cctgatggat atttagggca tgcaggcgat
     6541 cctgtcagta tttctgagaa tgtaaggcca ttaacaacct ttgaaagagc aagattgcaa
     6601 acatttcctg aagactttaa atttaaaggt gcaaaaacaa atttggagca aatgataggt
     6661 aatgctgttc cggttgagtt agcaaaatat gttgcggtga caatcatgga gtatg
//
LOCUS       DAZFJF010000201         6675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFJF010000201 DAZFJF010000000
VERSION     DAZFJF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.203"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(209..454)
                     /locus_tag="ACHKAU_004664"
     CDS             complement(209..454)
                     /locus_tag="ACHKAU_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533583.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRAR
                     ILKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
     gene            718..2811
                     /gene="cda"
                     /locus_tag="ACHKAU_004665"
     CDS             718..2811
                     /gene="cda"
                     /locus_tag="ACHKAU_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HFO5533584.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGS
                     GVSPLYISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            2808..3071
                     /locus_tag="ACHKAU_004666"
     CDS             2808..3071
                     /locus_tag="ACHKAU_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFO5533585.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
     gene            3141..3287
                     /locus_tag="ACHKAU_004667"
     CDS             3141..3287
                     /locus_tag="ACHKAU_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO5533586.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIG
                     VAVQ"
     gene            3994..4182
                     /locus_tag="ACHKAU_004668"
     CDS             3994..4182
                     /locus_tag="ACHKAU_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533587.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLC
                     GISHPAWCTFHTILTDAT"
     gene            complement(4334..4525)
                     /locus_tag="ACHKAU_004669"
     CDS             complement(4334..4525)
                     /locus_tag="ACHKAU_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO5533588.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4593..4916
                     /locus_tag="ACHKAU_004670"
     CDS             4593..4916
                     /locus_tag="ACHKAU_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO5533589.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            5125..5559
                     /locus_tag="ACHKAU_004671"
                     /pseudo
     CDS             5125..5559
                     /locus_tag="ACHKAU_004671"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5612..6154
                     /locus_tag="ACHKAU_004672"
     CDS             5612..6154
                     /locus_tag="ACHKAU_004672"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO5533590.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRD
                     AISAHTAAVKEQLEMLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVT
                     VGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSG
                     VKGENNWTVEKKNAVRLVRE"
     gene            6161..6394
                     /locus_tag="ACHKAU_004673"
     CDS             6161..6394
                     /locus_tag="ACHKAU_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO5533591.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
BASE COUNT         1662 a         1419 c         1936 g         1658 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 taaggggtta tgttaaataa cccttagctt agcggcggcg cagtggttgc caggtgaagg
      241 tgtcatccgg gtcctgtccg gcagtcagtg tggcttctcg atttttgctg gccgttcgtt
      301 catcaagaat ggctttaagt atccgtgccc ggaggcgtcg ctttgtttcc tgcggcattt
      361 tgtccttttt cgtcagcccc atggcacgag cttcagcggc aatggcttct gccagtcggc
      421 gctcttcggc ttcggtcagt ctatggcgtt tcatggtggt ctccgggtgt ttctctggtg
      481 tcataacagt ttaactgttg gctctctcct tatgctaatc agccttcttt catgcctgtt
      541 gccattgagt ttaagaatga ttggttgttt ttcttgagcg ttttcacaaa aacgtaaatc
      601 cagtgggttg acaggattta cgaggtgacg taacttttta ctgtacataa aaccagtggt
      661 tttatgtaca gtttgttgtt tttaacgcat tgttttaaaa gtcaaagagg tgtttttatg
      721 agtgattacg aaggtagtgg tccgacagaa ggtattgatt atgggcactc gatggtcgtg
      781 tggccgtcaa caggactgat ttcgggtggt gatgtgaaac caggaggctc atcaggtatc
      841 gctccatcca tgcctccggg atggggggat tacagcccac agggtatcgc acttgtacaa
      901 agtgttcttt ttccgggaat tattcgccgg attattctgg ataaggaact tgaagaggga
      961 gactggtcgg gatggtctgt cagtgtgcat agcccttggg gaaacgagaa agtttccgct
     1021 gcacgaacag ttcttgagaa tggtttacgt ggtggtttgc cagaaccgtc tcgcccggct
     1081 gctgtttctt ttgcccgtct ggagcctgct tccggaaatg agcaaaaaat tattcgtctt
     1141 atggttacac agcaactgga acaggtaacg gatattccgg ccagccagtt accagcagcg
     1201 ggtaataatg taccggtgaa atatcgtctg atggacctta tgcagaacgg tacgcaatat
     1261 atggctatta tcggaggtat tccgatgaca gtgccggtag ttgatgccgt tccagttccg
     1321 gaccggagtc gtccgggaac aaatattaaa gatgtttaca gtgcccctgt atcaccaaat
     1381 ctaccggacc tggtattaag tgtgggtcag atgaatactc cggttctgtc taatcccgaa
     1441 attcaggaag aaggagttat tgctgagaca ggtaattatg ttgaggctgg ttatacgatg
     1501 tccagtaata atcatgatgt cattgttcgt tttcctgatg gcagtggtgt ttctccgtta
     1561 tatatttcaa ctgtagagat tctggacagt aatggtctga gtcagcgtca ggaagccgaa
     1621 aataaagcaa aggatgattt cagagtcaag aaagaagaag cggtagcccg ggctgaagct
     1681 gaaaaagcaa aagccgagtt atttagtaaa gcaggtgtga accagcctcc tgtatataca
     1741 caggaaatga tggaaagggc taattcagta atgaatgagc agggagctct tgttctgaat
     1801 aacactgcca gttctgtaca actggcgatg accggaactg gtgtctggac tgctgctggt
     1861 gatattgcag gcaacataag caagtttttc agtaatgctc tggagaaagt aaccataccc
     1921 gaagtgagtc ccctgcttat gcggatttct cttggtgctc tgtggtttca ttcagaagaa
     1981 gctggagcag gaagtgatat cgtgccgggg cgaaatctgg aggcgatgtt ttcgctgagt
     2041 gctcagatgt tggctggaca gggcgtggtc attgaacctg gtgcgacgag tgtaaatctg
     2101 cctgttcgtg gacaattgat aaacagtaac gggcaattag ctctggattt actgaaaaca
     2161 gggaatgaaa gtatcccagc tgcggttcct gttcttaatg ctgtccgtga tacagcaaca
     2221 ggactggata aaatcacgtt accagcagta gtaggcgcac cttcccggac gattctggtt
     2281 aatccggtac cacaaccttc ggtgccaaca gatacaggta atcatcaacc cgttccggtt
     2341 acaccagtgc atacaggaac ggaagtaaaa ccggtcgaaa tgccagtaac gacgattact
     2401 cctgtttctg atgttggtgg actacgagat tttatttact ggcgtcctga tgctgctggg
     2461 actggtgttg aagctgttta tgtgatgctt aatgatcctc tggattcagg gcgattttcg
     2521 cgtaaacaac ttgataaaaa atataaacat gctggtgatt ttggtattag tgatacaaaa
     2581 aagaatcgtg aaactcttac taaattcagg gatgctattg aggagcattt atcggataag
     2641 gatacagtag agaaaggaac ataccgaaga gaaaaaggtt caaaagttta ttttaatcct
     2701 aatacgatga atgtggttat aattaagtca aatggtgagt tcttatctgg gtggaaaata
     2761 aatccagatg cggataatgg tcgaatttat ttagagacag gtgaactatg aacaagatgg
     2821 caatgatcga tttggcgaaa ttatttttag cttcgaaaat tacagcaatt gagttttcag
     2881 agcgaatttg tgttgaacgg agaagattgt atggtgttaa ggatttgtct ccgaatatat
     2941 taaattgtgg ggaagagttg tttatggctg ctgagcgatt tgagcctgat gcagataggg
     3001 ctgattatga aattgatgat aatggactta aggttgaggt ccgatctatc ttggaaaaat
     3061 ttaaattata atcatgagtg attttaatat ctggcattta agtataaatg tcagatattc
     3121 actcatgaca aggagatgtc atgaaaaaaa agataggggg atctatgatt ctcgttcttg
     3181 ctgttttgtg tctgactgct tgccaagcta actatgtaag ggatgttcag ggtggaacaa
     3241 ttgctccatc atcatcttcg aaactaatcg gggtggcggt tcagtgaaaa atattaaagg
     3301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ctgtaaacga aaaaacccgc
     3361 cttgtggcgg gtccttccgt ccgatcagaa gtcttcgacc tcttctaatc ggcggtggta
     3421 actggcttgg aggagcacag tcaccaaaac tgtcttttca gtttagcctt aactggttca
     3481 taacttcaag agcaactcct ctaaatcagt taccaatggc tgctgccagt ggcgttaagt
     3541 cgtgtctttt ccgggttggc cccaagagaa cagttaccgg agaaggtgca gcagtcgggc
     3601 tgaacggggg gttcctgcat acagcccagc ccggagcgaa ctgcctaagt cgccccgaga
     3661 taccagaaga cgtgagctat gagaaagcgc cacgtgtccc gcaagggaga aaggcggaaa
     3721 aggtatccgg taatcgatat ggcgggaacg tgggggcgca ggagggagcg attcaccgga
     3781 aacggtgatg atctttaagc cacgtcgggt ttcgccaccg ctggcggatt tcatggttga
     3841 gcctcacggc tcccacagat gcaccgaaaa agtgtctgtt ttgtgaagtc cgtcaggggg
     3901 gcggagccta tggaaaaagc gcctccggcg cggccttttt cggggttttc gtttcttgct
     3961 cacatgttct ttccggcgtt atcccctgat tctgtggata acactatcac cgcttttgag
     4021 tgggcagata ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     4081 gcggaaaagc gcctggacga gcattttctc cttacgcatc tgtgcggcat ttcacacccg
     4141 gcatggtgca cttttcatac catcctcact gatgccacat gattaagtca gtatacactc
     4201 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgctaaaa cccgctgacg
     4261 cgccctgacg ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg accgtctccg
     4321 ggagctgcat gtgtcagagg ttttcaccgt catccccgaa gcgtgcgagg cagctgcggt
     4381 aaagctcgtc agcgtggtcg tgaagcgctt cgcagatgtc agcctggtcg tcggcgtcca
     4441 gttcattcag tttttccagc agggtcagcg tctggcttct gatgaagcgg gccatgttaa
     4501 gggcggtttt ttcctgttta gtcacagttc tgtttctcca tgtgtgggga gtctgtcgga
     4561 ggtttttaat gataccggtg aaacgcgaaa ggatgctcac gatacgggtc acggatgagg
     4621 aacatgcgcg gctgctggca cgttgtgagg gaaaacaact tgcgtcatgg atgcgtaagg
     4681 tctgcctggg ggcaccgcca tcgaaaacgt caggtcttcc gacactggca ccgccgctgt
     4741 tgcgccagtt cgcttcggtc ggcaataacc tgaatcagat agcccggaag ataaacagtg
     4801 gacagtggtc ggggcatgac cgggttcatg tggtggctgc actgatggcc atcggacgtg
     4861 aactgagtga gctgagggat gaggtccgta aacagggaga acgggatgat agttaagttt
     4921 catgccaggg gaaaaggcgg tggcagtggt ccggttgatt acctgctggg ccgggagcgt
     4981 aaccgggaag gggcgagagt tctgcggggt gctcccgaag aggtgcggga actcatcgat
     5041 gcgacgccgt ttgcgaaaaa atacacctcg ggggttctgt cttttgcaga gcagaccctg
     5101 ccaccgggag agcgtgaaag ggtgatggag agctttgagc gggtactgat gcccggtctt
     5161 gaaaaaaatc agtacagcat cctgtgggtg gagcaccagg acaaggggcg tcttgagctg
     5221 aattttgtca tcccgaatat ggagctggcg agcggaaaac ggctccagcc ctattacgac
     5281 cgggcggacc gtccgcgaat caatgcctgg cagacactgg tgaatcacca ttacgggctg
     5341 cacgacccga acgcaccgga gaacaggcgg acacttgtca ccccgaataa tctgccgaaa
     5401 gcgaaacagg aggccgcaga agcgattacg aggggactgg aggctcttta ccatgccgga
     5461 gcgctaaaaa cgcgtcagga tgtcactgag gcgcttacag cggcaggttt tgaggttgtg
     5521 aggacaacca ggagcagtat cagtatcgcc gacccggagg gtgggcgtaa tctcaggctg
     5581 aaaggggcgc tgtatgagca atcttttaca aatggggaca gacttcgaga agaaactgaa
     5641 agagcgtgcc gtctctacag agaacatgct gaacagcgag ttcaggaagc tcgaagcgtc
     5701 tgtagacagg gaactgaact taaacgggca gaaaatcaga gacgctatca gcgcacacac
     5761 agcagcggtg aaagaacaac tggagatgct gaacagtgcc gtgaacgcgc agttcagcgc
     5821 cacagagaac gaacttgccc ggcggcagga agagctgctc tggaagctgg tcaaagggcg
     5881 gatactgtac ccgtcgctga cggcgctttg cgtcacggtc gggatttttc tggcgtcatg
     5941 ggggctgatt cagtggcagg aaagccgtat tgcggcgaac atcctggcta tcagggacca
     6001 ggaagagacg ctggcgaaac tccgggaaaa aacatggggt gtgacgtatc acgagggcag
     6061 gaacgggaaa tttctggtgc tgccgtccgg ggtgaagggg gaaaacaact ggacggtgga
     6121 gaagaagaac gcagtaagac tggtcaggga gtaagtgagt atgacagagc tggaaatgca
     6181 cttactgaac gccttagagc aattgcagca ggactatacg cagcggctga aagactggga
     6241 gagcgccttc acggatttgc agcatatgtt cgagctgaca cgacaggaga acgggcaact
     6301 gagggaacag tgcgacgact tgagtcagca ggtgcagaga ttagccgggc aggtggaccg
     6361 cttgagtcgg ttatttatca cgaacagcag ataagggatg aacaggaaag gcaggcacgc
     6421 ctggaacggg agcgtgcgcg gtcctggcat gaagagcagg aacgggaagc acaggtatgg
     6481 cgtggcccgt caatggggat gtgacggtga gttgttccgg gaggttgcca gatggtaccg
     6541 gaacgcgtta aaaacgcatc cgggcgcgat tctggcgtgt cgtttttgtt gctgtgtttt
     6601 gcctctttgc cagatcgcgc tcattcctca gcctcatccc gcgatgtctg cgtatgtcat
     6661 caactgaaaa gatgg
//
LOCUS       DAZFJF010000202         6664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFJF010000202 DAZFJF010000000
VERSION     DAZFJF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.204"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..105)
                     /locus_tag="ACHKAU_004674"
     tRNA            complement(29..105)
                     /locus_tag="ACHKAU_004674"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Arg,seq:tcg)
     gene            complement(115..190)
                     /locus_tag="ACHKAU_004675"
     tRNA            complement(115..190)
                     /locus_tag="ACHKAU_004675"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Ile,seq:cat)
     gene            complement(219..932)
                     /locus_tag="ACHKAU_004676"
     CDS             complement(219..932)
                     /locus_tag="ACHKAU_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5533592.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(1067..1264)
                     /locus_tag="ACHKAU_004677"
     CDS             complement(1067..1264)
                     /locus_tag="ACHKAU_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5533593.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(1506..2036)
                     /locus_tag="ACHKAU_004678"
     CDS             complement(1506..2036)
                     /locus_tag="ACHKAU_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5533594.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(2045..2404)
                     /locus_tag="ACHKAU_004679"
     CDS             complement(2045..2404)
                     /locus_tag="ACHKAU_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5533595.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(2417..3463)
                     /locus_tag="ACHKAU_004680"
     CDS             complement(2417..3463)
                     /locus_tag="ACHKAU_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5533596.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            complement(3465..3737)
                     /locus_tag="ACHKAU_004681"
     CDS             complement(3465..3737)
                     /locus_tag="ACHKAU_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533597.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            3873..4130
                     /locus_tag="ACHKAU_004682"
     CDS             3873..4130
                     /locus_tag="ACHKAU_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5533598.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            4136..4435
                     /locus_tag="ACHKAU_004683"
     CDS             4136..4435
                     /locus_tag="ACHKAU_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5533599.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(4640..4984)
                     /locus_tag="ACHKAU_004684"
     CDS             complement(4640..4984)
                     /locus_tag="ACHKAU_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533600.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(4981..5346)
                     /locus_tag="ACHKAU_004685"
     CDS             complement(4981..5346)
                     /locus_tag="ACHKAU_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5533601.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            complement(5348..5566)
                     /locus_tag="ACHKAU_004686"
     CDS             complement(5348..5566)
                     /locus_tag="ACHKAU_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO5533602.1"
                     /translation="MTTLFRKEYPRKSRATEFLFLIIFIVLMIPIPPLLLVWFIVKII
                     EPVIELYNDVVWASFSTLHNKINPYKES"
     gene            complement(5792..6289)
                     /locus_tag="ACHKAU_004687"
                     /pseudo
     CDS             complement(5792..6289)
                     /locus_tag="ACHKAU_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(6286..6462)
                     /locus_tag="ACHKAU_004688"
     CDS             complement(6286..6462)
                     /locus_tag="ACHKAU_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533603.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            complement(6455..6637)
                     /locus_tag="ACHKAU_004689"
     CDS             complement(6455..6637)
                     /locus_tag="ACHKAU_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533604.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
BASE COUNT         1665 a         1786 c         1406 g         1807 t
ORIGIN      
        1 ctgagctaac aacgcatgat gctgataatg gaccgccatc ggggacttga accccgcgca
       61 gccagcttcg aaggctggcg ctctgtcccg atgagctaat ggcggtatgt gatatggtgg
      121 cccttgctgg atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag
      181 ctaaagggcc gagccaaaaa ataataatca gatgaaaatc aataatcaag cccttgcctg
      241 gatacatatc tgtctggcgg gaagccataa tagcggtgaa atacagaaat aaagtaggac
      301 ctgcttgaat aaccgcattt ttctgctata gcctgtccat atccatgccg ggaacataac
      361 atatttacag caacacgcat ccgctcttcc agcaacaagc gactgaacat gcccccttca
      421 tttttcagtt ttgtctttaa caaactctca ctcatatgca actgtagagc aatcgcacca
      481 agcgtccagc ttgctgatat atctgtctga attatcgccc tgactttggc acttatgctg
      541 gataaacatc cacttaaaaa caatgacatc cgttcatctg tttcaaacag agacaggcag
      601 gccatcataa gaaacatatc cgtggcctct ccggaaaatc cctggctggt aattaaagcc
      661 gcagccaacg caggattgtt gggttccagc aacaggtaaa gcggaatgtc agtcagacga
      721 gtcctcgtca gcttatgctg actttccaga tattgactta cgacggattc gcttatatcg
      781 acaattttaa ctttgccata atgcataagg aaaagctccc tgatgcattt ggtggccaga
      841 acgactgagc ctggcttaag tgacaacgta tccttttcaa gaaaaatatt aattggggag
      901 caaaccatga taactgaaca gacaacagcc attataattt tactttcatt agcaattggt
      961 tagctcaatt atagccccaa aaggtaaatt atcatcaaca cataagcaaa ggactgacag
     1021 gtgtcgcccc cccaccagcc gcccattcac cacaaataaa aagccttcag gactgaaggc
     1081 gtctgtaaca accgcactga tagtctgcca gacccgccat aacaagctgg gtcagtatta
     1141 actggcagcg ttcgcgtgaa aggtaagtat tctgcgcaac ctccccgact gtcgccggtt
     1201 cggtaacgct taattcatta aacaccactc tggcggtttc ggtcatatcc tgctgtttca
     1261 gcatgtcttt ttcccttttt cggttaacgt gacacaccaa taactcttgt cgaaaaagcc
     1321 agcaagctga aagaacggta ttaataacca ccagcgaatt tattgcgctg ctgtatatta
     1381 cggacacaaa aaaaccacct tccggtggct tccttgtgcg aaaaaacttg catttcgcct
     1441 cgcgatacag ctttgcgaag cttacaggaa ttcaagctgt ttctgcgtaa aaaagcaagc
     1501 tttttttatc gaaatgaatc gtgcataggt acataaagca tgtgttcagc cacggctaac
     1561 caacctgcaa tacgtttctc acatgtgctg aaacaccatt ccgggtgagt acgatttaaa
     1621 cattctgcca tttttctctt actcattccc cgtccttcgt acctttgccg gagaatattg
     1681 attagcccgg gatattcccc aagcacctca ctgataacgc gatcaataat caacgcctct
     1741 gtgtctgtac aatgtgacaa ccagctcttc tgcttccctc tggtcatatc ccgaaaaaat
     1801 gcctcaagtt ccggtttttc cagcccggat ttcttcatgc tgcgtaaaac ctcattaact
     1861 gctgttttcg tcagcttttt cgaaaccagt aaccggttaa acatatttcc ggatttaccc
     1921 ccaccgatat acgaccaccg cccccacatc cgtaatttcc cctggatcca gactgcttcc
     1981 agcgtgttca ggcgtaaatg ttcgccgctt ttgcctgtaa tttccggata tatcatattt
     2041 acgctcactc actctcaatt ttgtaaatct tcacacccag ccgtccacca gatactggct
     2101 gaccgcgcac aatattgatt tcatcaaact gctcgtcatc aatgagcact tccgcatgcg
     2161 tcagcgcatc cagcggtgct ttcagaatgt tgtccaggtc acggcggcgc ttatccggtg
     2221 gctctgcaat cacctttatc gccagccttc cggacaggct taatttcagc cgctgctggc
     2281 gaacaataag cgccacagcc cggcgataac gctttccctc ctccgagata aaatatgtgc
     2341 tgccacggcg tcgccagtaa gtgttcaccg tcggcgggta aggtaaaacc aaatctatga
     2401 gcatcagtca cctcttttac ccaagcacgc cagttgcaaa ggcgtgatca agaaaacgaa
     2461 aaattaaatc aacctgagaa ccatgctttt cttcgaacgc cagcggatcc gcatgaagct
     2521 cgttgtgatg ctcccgacac agcggtagcg tgaaaatatc gtgagatttt gtccccattc
     2581 ccccctgacc atgaccaatc aggtgatgcg gatcgtcagc aggcttacca caacacgcac
     2641 acggctgtgt cttcacccag tgcgtgtatt tctcgttaac ccagcggcga cgtttaggtc
     2701 gtttcatgaa agattccgga gactcaggat caacggcaat gctgaccacc gtcttttcct
     2761 gtggcggttt ttgctggtga gcgtgaggca gcggcgcaag attttttgtg cgctgcttca
     2821 gtatgctggt ggcggtctgc tctcccggta cgatgtcgct ttcacggtac attgagcgga
     2881 ttttttccgc acgcaacccc agcgaacgac gtaataccgc ttccggtagc gcgtccgcca
     2941 cctgattgcg gaccgcccac caggataatt cagccagaga taattcccgt tcctgtgcac
     3001 cacttattgc gtgacgtatg acgtcaatca tccaggctgt cagattttgt tgagcaagct
     3061 gcccgagtga ttcggatgtc tggtcgcgca gctggttgtc gcagtgccag cacaacacca
     3121 ttgcgccggt accataacgg tgaatgacgg tttcgctgtg atgataatcg ccgtgtggcc
     3181 actggcagga tttaatatgg cgcaacagcc agtcagacaa tgcaccagca ccaccagcag
     3241 cacgaatcac ccgtgcgtta ctgaaaaacg gcagcaatgt tttgtcttcc actagcggct
     3301 ggcgaacggc aggaacaacc ccggacggca gattacgcat gcttttcggt tccggctcca
     3361 ccagtacccg ggtattgtgg aataccggca tggattcacg acccggctta acgatcacca
     3421 gcccgagttc cggtaccaga acaggtcgaa gtaatacccg cacgttacct ccagatccgt
     3481 tgctggaatg tgcgggacgg acgcggcggg cgttcggaat aaaggagcct gacgtagatt
     3541 atccagtgac ggtagtcgag gctaagggct ttcttaaact catacccacg tctgcggtag
     3601 ttatgaatca gccattcggc ctgttcttca gtgcagggat cgtgctgata ccagtcatat
     3661 ttgaatgtgt gagaacaccg cccttgcctg ctggcagggg cgatatcaga attgtgatgt
     3721 ttggtattgt gcgccatcgg ttttctctgc tggcgcagca ggtgccagtt gttcaagctg
     3781 gcgtgcggca atattgtctc tgatttctgt tgtcgtcaac aggcaacgtg ctatcatcga
     3841 atggtgttct atcctactcc gtgaggttta ccatgcgtac aacccaacaa ttcagcatta
     3901 cattaactaa cgaaatggct gacatggtgc gcgcccgtgt ggcttccggt gcctatgctt
     3961 cagaaagcga ggtcattcgt gaagggcttc gcgcactgaa tgagcgcgat aaagcaatcg
     4021 aagcgtggtt aacgcattca gccgccccct ctcttgattc tatccgcgaa aatccaaaca
     4081 acggacgctc catttcacag gttcgcgccg cgattcgatc cgggaagtaa tctgcatgac
     4141 atatgaagtc atcattactc ctgaggccga acaacaaata atcaacctgc acaggtatat
     4201 aacggagaaa gcagggaacg tcattgctga caattatgcc aatgcgcttc ttgattatct
     4261 tgatgggttt tctacattcc cgcatcgggg caataaacgc gatgatattc gccaggggat
     4321 gcgggtaact catttccgcc acagaacgat tattgctttt gccgttgata gcagaaaagt
     4381 ctttattgtc ggtatctatc atggtgggca aagttatgaa accgatttct tataaacttt
     4441 tacccacatc attccggtgt tagaattaac cgtccgcccc ctctcttact ggcggattcg
     4501 taggctatat aaatcaaaga tcccggctca tgtttatgtc gggatctttt ttcggcgatt
     4561 tatccccagc ggcaaatcga atacaccacc agcgccaccg ccatcgcaat tcctaccgtt
     4621 gtgaatgctt caggccaggt catcgtaaaa tatcctccac gcttatcagt ccgttccgct
     4681 ccagataact catcgcctta tccggtaatt tgcagtctgg cttcgctttc ctcagttgcc
     4741 aggttaactg ctttaccagc atggttaact catcgaccag acgctgatat cccactggtt
     4801 tgtattcatg caatttaccg gctggctctg ctgccagcga taccagtgcg atttccagaa
     4861 cagcaatatc catcttatat gtgcggatga tgtcatggtc gattgtgccc ggtatgcaca
     4921 gtctctgtgt ttcaatagtc tcctctgcgt gagctattaa ctgctctctg gtaaaagtcg
     4981 tcatgccgta gccccttctt gatatttttc aaaccagaac acaactgggt cagatttcat
     5041 ttcaaccaat cccatacgaa ccagcgcttt gcctttcccg gacgcaagga attcacgacg
     5101 accatcactg ataattcgcc gataatcttc cagactactg caatgcttgt gcagattgca
     5161 tgggtggcat gccggaacca tgttggatat atcgtcacgt tcctggtgaa gcatatttcc
     5221 atcaaaacga atgaccggtt ttacatggtc tgcatgccac ttttcgccaa gttcgcagcc
     5281 gcaataagca cagcgaccgc cgaacttcat gcgcagctct gcacgttgtt ttttcgtcag
     5341 tgccatatca gctttcctta tacggattaa ttttattgtg cagtgtgctg aacgacgccc
     5401 acaccacgtc gttatacaat tcaataactg gctcaattat tttcacgata aaccagacca
     5461 gtaatagcgg gggtatcggt atcatcaaca cgataaatat aatgagaaac aaaaattctg
     5521 tcgctctact ttttctcgga tattcttttc tgaataatgt agtcatttct taccgccctt
     5581 tcaggcggcc tcctgatgtt ttgagggtgc agaaatccct ccggttaagg attaaatttt
     5641 taactaaatt taattattca ggcgcgataa gctgttccgc acaatccagc aatgcttctg
     5701 tcgcttcctt aagcgttact gtatcgctat catccagtcc tgcaactttt tcgtacctga
     5761 caaacgccgc gcaaagctcg ttaaacaacc ccgcccgtac ttcagccagg aaagcgtcgg
     5821 tggccggagt ttcaggaatc cgtctcctca tccgttctat tgcatgattg aacccgaaat
     5881 cttcagcaag agatacgtca tccatattgt cattgtcgtc ctcaatatcc cgtggttctg
     5941 gaattgcaga ctttattacc gcattctccg ccgccagcgc cacaagatta gctttcagct
     6001 ctgcaattcg tttgttttgg gcttcccgtt catccagtag tgccagcgca acatttggat
     6061 taaaagcagc aataaattca gcgtttgcat aagcctgaac atctgtttca accaggcagt
     6121 taacatgaca ttctgcaatt acgccaccgg gctctccttt ccatttttga caaacaaaaa
     6181 ctcctgttat attcccatgc tgattgcccg atgtatgccc tacgatgtag catcctttag
     6241 ttgctttctc tgctgcttca cgcagcgcct gatagtcaat ctcgctcatt catcgcccca
     6301 ctcatcacaa tatgcttcga ccggattttt tcctgcttca taatcatcac gccatgcttc
     6361 agcatcagca gcactgccac cacgtaactc tgcatattcc attaacagtt catgccattc
     6421 ttcaaaactg acgctgtatt tagttgaacc aaaatcagcc attttgttct tcctcttcgt
     6481 cttttatttc gtgatatgaa taattgcagt agttaaagaa aatatctttt gcttcgtcat
     6541 gtatttcatc aggcgtcgca tcatcatcca cttcgaattc atcctcgaaa tctccaccgg
     6601 ctattcccgt ttcaataatt attttaaact ttcgcattta actaccgccc tttcgggcgg
     6661 cctc
//
LOCUS       DAZFJF010000203         6630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFJF010000203 DAZFJF010000000
VERSION     DAZFJF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.205"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..919)
                     /locus_tag="ACHKAU_004690"
     CDS             complement(<1..919)
                     /locus_tag="ACHKAU_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
                     /protein_id="HFO5533605.1"
                     /translation="MIKETVTMSHKELHRLQIIQESINRHITQEQAAARIGISIRQVK
                     RLVQRYRNEGPSGLVSHRRGKRPNNSFSTEFRATVISLLKGRYADFGPTFACEKLREI
                     HGLSLSVETLRKWMIEEGLWRERRRKIARIYQRRMRRPSYGELIQIDGSPHDWFENRG
                     PRCTLIVFIDDATSALMALRFVPAETTRAYMETLRGYLNDHGVPLALYSDRHSIFRVN
                     NPEREGELTQFTRAIKTLGIEPIHANSPQAKGRVERANQTLQDRLVKEMRLQNISDIE
                     TANAWLPTFIEAYNNRFATSPRTTDNAHLD"
     gene            complement(953..1372)
                     /locus_tag="ACHKAU_004691"
     CDS             complement(953..1372)
                     /locus_tag="ACHKAU_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5533606.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            complement(1413..2378)
                     /locus_tag="ACHKAU_004692"
     CDS             complement(1413..2378)
                     /locus_tag="ACHKAU_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533607.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(2359..2880)
                     /locus_tag="ACHKAU_004693"
     CDS             complement(2359..2880)
                     /locus_tag="ACHKAU_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5533608.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(2864..3091)
                     /locus_tag="ACHKAU_004694"
     CDS             complement(2864..3091)
                     /locus_tag="ACHKAU_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5533609.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            3169..3576
                     /locus_tag="ACHKAU_004695"
     CDS             3169..3576
                     /locus_tag="ACHKAU_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5533610.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            3769..3924
                     /gene="ydfA"
                     /locus_tag="ACHKAU_004696"
     CDS             3769..3924
                     /gene="ydfA"
                     /locus_tag="ACHKAU_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5533611.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            3926..4027
                     /gene="ydfB"
                     /locus_tag="ACHKAU_004697"
                     /pseudo
     CDS             3926..4027
                     /gene="ydfB"
                     /locus_tag="ACHKAU_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            4988..5176
                     /gene="dicB"
                     /locus_tag="ACHKAU_004698"
                     /gene_synonym="ftsT"
     CDS             4988..5176
                     /gene="dicB"
                     /locus_tag="ACHKAU_004698"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5533612.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            5173..5364
                     /gene="ydfD"
                     /locus_tag="ACHKAU_004699"
     CDS             5173..5364
                     /gene="ydfD"
                     /locus_tag="ACHKAU_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO5533613.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            5458..>6630
                     /locus_tag="ACHKAU_004700"
     CDS             5458..>6630
                     /locus_tag="ACHKAU_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5533614.1"
                     /translation="MSKVFICAAIPDELATREEGSVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFY"
BASE COUNT         1791 a         1628 c         1478 g         1733 t
ORIGIN      
        1 catcaagatg agcattatca gtagtacgag gcgacgtagc gaaccggttg ttataggctt
       61 caataaaggt cggcaaccat gcatttgctg tttcaatatc actgatattc tgaagccgca
      121 tttctttgac cagcctgtcc tgtagtgtct gattggcgcg ctctacccgc ccttttgcct
      181 gcgggctgtt ggcatggatt ggctcgatgc ccagtgtctt tatcgcacga gtgaactggg
      241 tcagctcacc ttcccgctct gggttattta ccctgaatat actgtgtcta tcagagtaga
      301 gagcgagcgg tacgccatga tcattaaggt aaccccggag ggtttccatg taagcccggg
      361 ttgtttcagc aggcacaaaa cgcaacgcca tcaacgcact ggtggcatca tcaatgaaaa
      421 cgatcagtgt acatctgggg cctcgatttt caaaccagtc atgaggtgag ccatcaatct
      481 ggatcagttc accgtaagat ggtcgtcgca tgcggcgttg atatatacgg gcaattttac
      541 gacggcgttc acgccataac ccctcttcta tcatccactt tctgagagtt tcaacggata
      601 aagataaacc gtgtatctcg cgcaattttt cgcacgcaaa cgtaggtcca aaatcagcgt
      661 aacggccttt gaggagtgaa attactgttg ctctgaattc agtagaaaag gaattattag
      721 gacgctttcc acgtcggtgg gaaaccagac cagaaggccc ttcatttcta taccgttgca
      781 ccagacgttt aacctgccga atagaaatgc caatgcgtgc cgcagcttgt tcctgagtaa
      841 tatgtcgatt aattgactcc tgaataatct gaagtcggtg gagttcctta tgactcatcg
      901 taacagtctc tttgatcatg aaaaatcccc cagagaattg tctggggaca ttttagaatg
      961 gttcaaaggg gacattacag cttggtgtta acaatagcct gcttctcctt gccttccggc
     1021 actcgcgaca gattatgttt agacgactgt cgtagcgacg tatttctccg tcaggtaatg
     1081 accagataag gtccggatca accacagcag gtttcttcac ctttgccctc gagagttttt
     1141 tgcgggcgtt ttgccagtcc ttacgcgcct gttcagacgg gaataacccg taaccagagc
     1201 tgtatacatc gccactggca accagctctc tggccagaac gctcatcaga tatcttgttg
     1261 ccccagtttt agcttccagt tgtcgtaacg tctcgcgccc actctggcgt acgagttcca
     1321 ccacctgccc tttaattttt tcccgctctt cttgtgtaaa aacttttgcc acaagtcctc
     1381 ctgaacatta ctttataccc taaaatcaac acttatcccc tgaaaccagg cggaatttct
     1441 gtatccggtt cagaaatatg attcacacaa cgctgtacag gtgaacgtcc caggcggatg
     1501 accagttcgt cccatttttc gcggagtttt gacgggctca tgatgttttt tacccagaat
     1561 tgatctcgct gtacccgacc aaacatttcg caaatttgtc tgtggcttct gccatccagc
     1621 atccgcattg tgcgcacgtc attggcccag gctgtccagt taggctcttt tggtcgcatg
     1681 atctctccat cgtcgctggc agcctgttcg taaaggttca cgatccgccc ccaaatccac
     1741 tgcgcacacg ccaaatcctc cctgctaccc cactggcgtt ttttcgcact aaacacaacc
     1801 gcgtcggggt tccgggttaa aaaatcctgt tcagcggtct gctggtccgg ttgcgaagct
     1861 tccggacgaa aagtgttttt attctctgta gtaatctctg ttgtattctc tgtaagatca
     1921 tcaggccatt ttgacccgat gacattgggt cgttttgaac caatggagcg tttcattttg
     1981 acctcttcca tcgtgtcatt ttgacctgat ggagcggcgc attttaaacc gatggattcg
     2041 ctcaatttgc caccatctaa aagctcgctc ccgtagttga tcgtgtagaa attggtcata
     2101 tcgcgctttg atttattgag cttttcacaa cgcaaaagcc ccagcgtttt cagacttgca
     2161 aacgcgcgct ttaacgttga ctctgaccag aatgggaact gttccagcca ttgttccgtt
     2221 gtgttgtaaa tccagcgaac accatcacat tccatgccgg aattggtatc tctcaaccag
     2281 taatgcaact gctgcaacac aatggcttca tttaagccaa tcttcatcgc aagctgtgtg
     2341 tttataacca gtgggcgttc agcaaaaaga aggctcataa ttccatccag ctttttgttg
     2401 gtattgctgt cgatacgcaa gtttgaaagc aattgctttt tctataagtt cgtcagtttc
     2461 acgatccact acggcaggat cagcaaaaag cagtccggac tccaccacat cgccatattc
     2521 tttgtttaac ccggcgatca tgtacgtgat gctttttccg tcactaattt cacgatacaa
     2581 cctgaaatca ttaatccgaa tagcctccat aattgcaggc actagcgccg tgaacttttc
     2641 acgcttatcc ctggtgtcga tagccttcca gcgttcgaat atcttcactc gattaacgcc
     2701 aagtgctcgc tgatcaaccg cgccaccttc atctgtgaca cgctgaacat cgatgttcgg
     2761 gcgctctttc aaagcccaga atgcttcagt gattaatatc gtcgcctgct cctgtgtcat
     2821 tcctggtcga catatccagg catccagagc ctcacgagcc tgttcaggag tgattttcat
     2881 tgttcaaccg ccccgcccgc ttcgtcttac gatattcgtc ataaaccttg ggatcataca
     2941 gaagctcgcc gccagatgcc tcctgtagac gcatcgcgcg accttcagga accagctctt
     3001 gccattgaga aacagcagat gggtcaacgc cagcagcttt cgctactttg gctttcgtac
     3061 cgtaaaaatt aattacgtct gatttaaaca tcgcacctcc aatattgagt tttctcaatg
     3121 ctaatcactc aaggaatctc aagtcaaggg ttattaagat atctaaatat gaacgagaaa
     3181 actttaggtc aacgcattag agaaagacgt aagcaggtag gcttaagtca aaacggttta
     3241 agcaaagctg ctggcgtatc tggctcatca atttcattat gggaaagtga ccatacagcc
     3301 ccgcgcgggc aaaatttgca tcgcctcgcc gaggtattgc aatgttcacc aacttggata
     3361 ctgtttggcg acgaggacaa aacaccagct ccccctgttt cactcgatag tgccttagac
     3421 ttatcggaag atgagttaga gatgctgcgt ttgtatcgcg cacttcctaa atcagagcaa
     3481 caagcacaac tcagcgaact ccgcgcccgc gttgagaatt ttaatcgcct gttcaccgag
     3541 ctattagagg ctcgcaaacg caacaagcat caataatcat cccttcacaa aattttaaag
     3601 ccttacattt caatgtattg gctttatctt gcgccaacac ttgagttttc tcatcaaaaa
     3661 accttgacga aaaataatga gaaaactaaa ttacctccat caagacaccg cacggtgttc
     3721 tcagcaaaca gttccgctac cccggcgtta aggggaaatg aggtcagcat ggatactatc
     3781 gatcttggca acagcgaatc tctggtatgt ggcgtgttcc ccaaccagga cggcacgttc
     3841 accgcgatga cgtataccaa aagcaaaacg tttaaaaccg aaaatggtgc ccgtcgctgg
     3901 ctggaaagaa actcaggtga gtgatatgga tttcaacaca atcatggaaa aggcttacga
     3961 agaatacttc gaaggccttg ccgaaggcga agaagctctc agcttcagtg agtttaaaca
     4021 ggcactcaga ataagaatgt gctctcacaa tgacgcggag cacaaatatg agaagcaaaa
     4081 tcagaccgca gaaaattttg ttctggaacc cggagaaacg cttttcaaaa ttcccgttac
     4141 gtgccccatt tgcggtttta catcagaaga acttgacgac tcctgtaaca atcaggaaac
     4201 aaccaagtat gtcgaagatg ataccgagtg cgcacgaaga acgattatat ccacgagtcc
     4261 aaactccagg accaataaat ctcactatga gagggtgatt aatccactcc cccaaaccaa
     4321 taaaaaagat gccggaggac aaaaaaccca atggaacaac gggatatctg tcaaaaaaag
     4381 acgttccatt aaagacaaac aacgcagcgc caacaactgt aagcgcttta taccagtaat
     4441 caattttcat gttcttaagc ggatttattg gtggttgcga cattgcttaa tgaatcctta
     4501 aaactgtggt gattttaagg ataccacctc gcctgacgtg gttaaaagca ggcacacaac
     4561 acgaaagcgc acggcgaagt tcgtctcact gtacggtgtc gttaaattta attcgaccgt
     4621 gcgcttccgg ttgtggcaac ccgcaaaatg gcgcggcggt aagtatggcg gggttattcc
     4681 ttccccgttg aggacaccgg gttgtcaggt tgaccatacg cttaagtgac aaccccgctg
     4741 caacgccctc tgttatcaat tttctggtga cgtttggcgg tatcagtttt actccgtgac
     4801 tgctctgccg ccctttttaa agtgaatttt gtgatgcggt gaatacggct aagcgcacgc
     4861 ggaacagtta aaaccaaaaa cagtgttatg ggtggattct ctgtatccga cgttaattgt
     4921 taactggtta acgtcacctg gaggcaccag gcactgcatc acaaaattca ttgttgagga
     4981 cgcgataatg aaaacgttat taccaaacgt taatacgtct gaaggttgtt ttgaaattgg
     5041 tgtcactatc agtaaccctg tatttactga agatgccatt aacaagagaa aacaagaacg
     5101 ggagctatta aataaaatat gcattgtttc aatgctggct cgtttacgtc tgatgccaaa
     5161 aggatgtgca caatgaattc agcatttgcg cttgttctga cagtttttct tgtttccgga
     5221 gtgccagttg atattgcagt cagtgttcac aggacaatgc aggagtgtat gactgcagca
     5281 accgaacaga aaattcccgg taactgttac ccggtcgata aagttattca ccaggataat
     5341 atcgaaatcc cggcaggtct ttaaaacagt tccgtaataa atatccggtt tcattcttat
     5401 atgccagcaa tggcagggat ttgttcatcc ttaaatctgt catgaggtta aaacaaaatg
     5461 agtaaagtct ttatttgcgc cgctattcct gacgaactgg caacaaggga agaaggctct
     5521 gtggctgtag ccacagccat tgaagctggc gacgaacgcc gtgctcgagc aaaatttcac
     5581 tggcagttcc tggaacatta tccggctgct caggactgcg cttataaatt tattgtctgc
     5641 gaggataaac ctggcatacc ccgccctgcc ctcgattcat gggatgctga atatatgcag
     5701 gaaaaccgct gggatgagga gtctgcttct tttgtcccgg ttgagactga atccgatccg
     5761 atgaacgtca cttttgacaa gctggcccct gaagtacaga acgctgtcat ggttaagttc
     5821 gacacatgtg aaaacatcac cgttgatatg gttattagcg cacaggaatt gttgcaggaa
     5881 gacatggcaa cattcgacgg acatatcgtt gaagcgttga tgaaaatgcc agaagttaac
     5941 gccatgtatc cggagcttaa gttgcacgcc attgggtggg ttaagcataa atgtattcct
     6001 ggtgctaaat ggcccgaaat tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt
     6061 gaacgcaagg aaaccggaaa atacacgtct gttgttgatc tcgcccgcgc cagagccaat
     6121 caacagtaca ctgaaaattc aacaggaaaa atcagcccgg tcattgctgc cattcatcgc
     6181 gaatacaagc agacatggaa aacactggat gacgaactgg cctacgctct ctggcctggt
     6241 gatgtggatg ccggaaacat tgacggcagc atccatcgct gggcaaaaaa agaagttatc
     6301 gacaacgacc gcgaagactg gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc
     6361 cttcgctacg accgccaaac tatttttggc cttgtccgtg agcgtccgat cgacattcac
     6421 aaagatcccg tagcactgaa caaatatatc tgcgaatacc tgacgacaaa gggcgtgttt
     6481 gagaatgaag aaacagacct gggcactgtt gatgttctcc agtcatcaga aacacaaact
     6541 gatgcagtgg aaactgaggt atctgatatc ccaaaaaatg aaaccgcgcc ggaagctgaa
     6601 ccatctgtag agcgtgaggg gccgttctat
//
LOCUS       DAZFJF010000204         6561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFJF010000204 DAZFJF010000000
VERSION     DAZFJF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.206"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..1496)
                     /gene="gndA"
                     /locus_tag="ACHKAU_004701"
     CDS             complement(90..1496)
                     /gene="gndA"
                     /locus_tag="ACHKAU_004701"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO5533615.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1607..2014)
                     /locus_tag="ACHKAU_004702"
     CDS             complement(1607..2014)
                     /locus_tag="ACHKAU_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFO5533616.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(2005..3213)
                     /locus_tag="ACHKAU_004703"
     CDS             complement(2005..3213)
                     /locus_tag="ACHKAU_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5533617.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            complement(3213..4343)
                     /gene="wecB"
                     /locus_tag="ACHKAU_004704"
     CDS             complement(3213..4343)
                     /gene="wecB"
                     /locus_tag="ACHKAU_004704"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5533618.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(4340..5446)
                     /locus_tag="ACHKAU_004705"
     CDS             complement(4340..5446)
                     /locus_tag="ACHKAU_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HFO5533619.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIANDEAIT
                     INDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLIIEDVGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            complement(5448..6482)
                     /gene="fnlA"
                     /locus_tag="ACHKAU_004706"
     CDS             complement(5448..6482)
                     /gene="fnlA"
                     /locus_tag="ACHKAU_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     4,6-dehydratase/5-epimerase"
                     /protein_id="HFO5533620.1"
                     /translation="MFKNKTLVITGGTGSFGNAVLKRFLDTDITEIRIFSRDEKKQDD
                     MRKKYNNSKLKFYIGDVRDYNSVLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVL
                     GTENVLEAAIANGIKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDM
                     GDYYRVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRAL
                     RSGEDYELDS"
BASE COUNT         1931 a         1482 c         1252 g         1896 t
ORIGIN      
        1 gcagtgaaat ttagtctata agataattgg caaaaaaaaa gcccggcgta atatacgccg
       61 ggcctcaatt tattgttggt taaatcagat taatccagcc attcagtatg gaacacacct
      121 tctttatcaa tgcgcttata agtatgcgca ccaaaatagt cacgctgagc ctggatcagg
      181 ttcgcaggca gaacagcggc acggtagctg tcataatagg caaccgcagc ggcgaaggtc
      241 ggaaccggga taccgttctg tactgcataa gcaacgacat cacgcagcgc ctgctggtag
      301 tcatcggcaa tttgcttgaa gtacggagcc aacagcaggt tagcgatctg aggattttcg
      361 gcataagcat cagttatttt ctgcaggaac tgcgcacgga tgatgcagcc agcacggaaa
      421 atcttcgcga tttcgccgta gttcagatcc cagttgtact cttcagacgc tgcgcgcagc
      481 tgagagaagc cctgagcgta agaaacgatt ttgcccagat acagcgcacg gcgaactttt
      541 tcgatgaact cagccttgtc gccagctggc tgcgcttgcg ggccagagag aactttagat
      601 gcggcaacac gctgatcttt cagagaagag atataacgtg caaacacaga ctcggtaatc
      661 agcgacagcg gttcaccgag atccagcgcg ctctggctgg tccatttacc ggtacctttg
      721 ttagccgctt catccaggat cacatcaacc aggtagttac cgtcttcatc ttttttggtg
      781 aagatgtctt tggtgatgtc gatcaggtag ctactcagtt caccgttatt ccactcggta
      841 aaggtctgtg ccagttcttc gttagagaga ttcaggccgc ctttcaggag agaataggct
      901 tcagcaatca gttgcatatc accgtattca ataccgttgt gaaccatctt cacatagtga
      961 cccgcgccat cagcaccaat ataggtaacg cacggttcgc catcttcagc aacggcggcg
     1021 attttagtca ggatcggtgc aaccagttca taggcttctt tctgcccacc aggcataatg
     1081 gaaggacctt tcagtgcgcc ttcttcacca ccggagacac cggtaccaat gaagttaaaa
     1141 ccttctgcag aaagctcacg attacgacga atggtgtcct ggaagaaggt attaccacca
     1201 tcaatgatga tgtcaccttt atcgaggtat ggcttaaggg aatcaatagc agcatccgtg
     1261 cctgcacctg ctttcaccat taacaggatg cgacgaggcg tttccagaga ttcaacaaac
     1321 tctttcaccg tatagtaagg aaccagtttc ttgcctggat tttcggcaat cacttcttcc
     1381 gtcttttcac gggaacggtt gaaaatagag acggtataac cacggctttc gatgttgagc
     1441 gcaaggttgc gccccatcac tgccataccg actacgccga tctgttgctt tgacattgtt
     1501 tactcctgtc aggggtggtc acgcggggtt acgcggcttg aagaagctaa gatggtaacc
     1561 cagcatgtat agaataagat agaagaataa acggtggtgt atcgccctat aaccacttag
     1621 aaaaagcctt agctttcagt gggtcaaatg gcccctcctt tatctctaac ataagggctt
     1681 ttggtgacag gcatttgaca ctatgtatat ctcctgggga aaattccacg acacttattc
     1741 ccgtaccgtc tccaacaaca aacttttttt ggagctcacc attttgctca tacaaacaaa
     1801 cttctaattg cccttgcatg acgacaaaca tttcccactg atgcggtaac tcatgccaat
     1861 ggggttcaac atagctgtcg cgtacaaatg caataagtaa acgttgaacc ttctcctgat
     1921 gagatgcgtg caataaataa tgagcgcgca aacgttcaga atttgctgca gtgcgaaaaa
     1981 gttcatccat aatattgtca tcaactaaac gcattctcct gcctccagtc ggacttcgat
     2041 agtatgtgcc gccgattcaa ccgaaaattg agactttaac aacacattgg cgttctgacc
     2101 taactgtttt cttaaaactg aatcactaag aagcaattgt gcagattcaa acagtttatc
     2161 atcttcacca ttaacatgaa tgaacccagc cctgtgctta ttaattacat ccattagatc
     2221 attaccgcca ttcacactcc caaggatcgg gattgattga accatatacc ctagtaattt
     2281 tccggggaaa ttatgtgaag aatggcgaga cgaaagggag aacaggccaa catcaacttc
     2341 agataaaatt aatttaaact cttcctggtt cactgaaggt agatgagtga aattagttaa
     2401 attccattct gcagaaagag atttaatcag gtcaacttca tcaccctgcc ctataaacag
     2461 gaaatgagca tcatgatgac gcatcatatt acgcgcaagg cgcattaagt ttgccatatc
     2521 ctgagcatgc ccaatattac cgccatagaa aaaaatgatt ttatctttta gagcgtattt
     2581 ttgacgaagt gaatgataat catcgccggc agacacagga gtcattgagg cccaattacg
     2641 taaaacttca cacggataac ctttattggc ctgacgaaat atctcaagat tcttatcaga
     2701 cattaaccca atccagtcag cctgttgata tgattttttt tcaaaatacc tgaaatattt
     2761 ttcaattggc gagccggctt ttatcatacc agcatcaatg acccactgtg ggaacatatc
     2821 ccttaggacc agatagcttg ggcactggca tcgctcttta atttttttaa ccaagcctcc
     2881 ccaaaaaata gagggggaat aataaacgat accatcaaat gtatcatgtt ggatgagatg
     2941 ctttaatgcg tgccatgcac gaaaagataa aagagtttca tttatggcac gtttagcctt
     3001 acctatatcc tttaaaggtc cacttttgaa acgccaaacc tttataccat cagtcatgct
     3061 aacagaatag attgcctgta atgtgttgtc aggcgtaatt acagttacat catggcctct
     3121 gctcagcaat tccaggccta actcatgaaa cattttagcc ccaactcgtg tgctatgggg
     3181 caaataatca tcaatgatta atgcaagttt cattagtatt gcttccagac aacccgttta
     3241 acgtagtcag tatatgaatg gataatacgc acaactttat ctgaaacatt tggcatacta
     3301 tagtcactga cctgacgaag taagcgtact tctccacgag gctgtgttgc aataatttct
     3361 aatgcctgta acacacgggc agactcaaga ccaaccatca ttacagcccc ttcttcgaag
     3421 ccttcagggc gttcgtgcgc ttctcgtata ttgagcgcag ggaagttcat aatggaggac
     3481 tcctctgtaa tagtcccgct atccgataaa acagcacgtg catttttttg cagatggttg
     3541 taatcgtgaa atcctaatgg cttaagtagc aagatatttt tatgaaattg aataccgttt
     3601 tcgttgatgc ggttacgagt gcgcggatga gtagaaacaa ctaccgggat atcatatttt
     3661 tcagccacgg tattaagtat atttgccagt ttcgcaagct gtttaggggt atcaacattt
     3721 tcttctctgt gggcacttac cacaaagaaa ttcccaggtg ttaaattcag gcgagaaagt
     3781 acatcggaac catcaatctg cgacatataa tgagtgagta cttcaaacat tgggctaccg
     3841 gttttaataa tcctatcggc tggtacacct tcagccaaaa gatattcacg cgcgatatca
     3901 ctatatgtca tattgatatc agcggtgtgg tcaactattt ttctgttagt ttcttccggt
     3961 acgcgttgat caaaacaacg attcccagcc tccatatgga aaatcggaat tcttcgacgc
     4021 tttgctggta ttgctgaaat acaggagtta gtatcgccaa gaactaacat ggcttctggt
     4081 ttttcctgtt caaagacctc atcaacttta atgataactt gtccaatagt ctctgctgca
     4141 tttttacctg cggcattaag aaaataatca ggtttgcgaa cacccaaatc cttgaaaaaa
     4201 acctcattca attcataatc gtaattttgc ccagtatgaa caataaggtg atcacaatat
     4261 tcatctaatt ttgcaaggac acgcgagagt cgaataattt ctggacgagt cccaacaacc
     4321 gacatgactt tcaatttttt cacgataaaa ctctcgctat agtatctggt tcagaacgat
     4381 taaatatttc atttgcccaa agcataacaa ctagctcatc cgagccatta ttagtaatgt
     4441 catgcgtcca tcccggaact gtttcaacaa ttttaaaatc atctgatgaa acattaagtt
     4501 catatcgttc acccgtgaca atattttcaa atttgaaaca agcacttcct cggatgataa
     4561 taaatttctc atttttggaa tgatgataat gccccccccg agtaattcct ggatgcgcag
     4621 taaaaaacga aaactggccc gcgtttttcg ttttcaatac ttcacagaat acccctctgt
     4681 catcactata agaaggaacc gtatacgcaa actgtttagg agaaaggtaa cttaaccatg
     4741 ttgagtacaa tgctcgagtg aatccattac ctacatcttc gatgattaat gtttggcgac
     4801 tttctttaaa tcgataaatt aattgtgcca cctcaccaac agtaacagaa taaattggac
     4861 caaatgtcct gtaaccagtt tcgttcgctc cttctaatag ctttaatatg tcagaacaaa
     4921 agtcatctat atacaccaaa tttacaactg ctgaaggatc attaattgta atagcttcat
     4981 catttgcaat gcgatggcag aaagttgcta taaaggagtt ataatttggt tgacaccact
     5041 taccgaatac attcggcaag cgataaatat aatatttagc gtttgtcgtt tccccatact
     5101 gctgaatgat tttttccgca actgctttac tctttccata agcgttatca cattcagctt
     5161 ggatggatga actaagcatg ataggagtat ctttattatt ttttttcaga atatcaacaa
     5221 tctgttgagt tagatcgcaa ttcccctctt caaactcact ctccttcaca ggacgattta
     5281 ctccagcaag atgaaaaatg aaatctgcct gcttaagtcc ctgctctaaa tccgtcaaag
     5341 aagagttacg atctatcgca ataagttcgt tatatccagc ttccttaagg cggaaaacca
     5401 aatttcgacc gataaaccct gcagcgccag taactaaaat tttcatatta tgaatccaac
     5461 tcataatctt caccagaacg aagtgcccgg ataaaaggaa gttttagcag taattctttc
     5521 attccctcaa catctaatct ctcagtatta tgagaattat aatcttccac ttccgagata
     5581 cgacggtcac catgttccac atattttcca tagttcaaat cgcggagatc tggtggaaca
     5641 cgataataat cacccatatc ttccgctgca atcatttcct ctcggctcaa taacgcttcg
     5701 taaagttttt ccccgtgtcg agttccaata atattgattg gatgctcatg ggcatttagc
     5761 aattccttaa gtgcaatggc taatgtttga attgttgccg caggtgcttt ctgaacgaaa
     5821 atgtcaccat tatttccatg ttcgaaagca taaaggacca gatcgacagc atcctcaagc
     5881 gtcatcatga aacgagtcat attgggatcg gttatggtca atggtttacc agctttgatt
     5941 agatcaacaa ataatgggat gaccgatcca cgtgaagcca ttacatttcc ataacgagtt
     6001 ccacagataa ctgtttttga actgtcaaga ttacgtgatt ttgcaacaat aactttttcc
     6061 atcattgcct tagatatgcc cattgcattg ataggataaa cggctttatc ggtactcaag
     6121 cacaccacgc gtttaatccc attcgcaata gcagcctcca gaacattttc cgtacccaga
     6181 acatttgtct taaccgcctc catagggtga aattcacatg aaggaacttg tttaagggct
     6241 gctgcatgat acagaaaatc ggcaccacgc gttgcattta gaacggaatt atagtctcgc
     6301 acatcaccta tataaaattt taattttgag ttattatatt ttttccgcat atcatcttgt
     6361 tttttttcat ccctgctaaa tattcgtatt tcagtaatat ctgtatctag aaaacgctta
     6421 agtacggcat taccaaaaga accagtgcca ccagtgataa cgagtgtttt attcttaaac
     6481 atattttcac cttttacaga tattttttat agtcgtaata tgagcttttc atatagccat
     6541 attttgtttt agaagcaact t
//
LOCUS       DAZFJF010000205         6544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFJF010000205 DAZFJF010000000
VERSION     DAZFJF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.207"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            271..420
                     /gene="ydfW"
                     /locus_tag="ACHKAU_004707"
     CDS             271..420
                     /gene="ydfW"
                     /locus_tag="ACHKAU_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFO5533621.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(857..1189)
                     /gene="flxA"
                     /locus_tag="ACHKAU_004708"
     CDS             complement(857..1189)
                     /gene="flxA"
                     /locus_tag="ACHKAU_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO5533622.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(1392..1697)
                     /gene="ydfV"
                     /locus_tag="ACHKAU_004709"
     CDS             complement(1392..1697)
                     /gene="ydfV"
                     /locus_tag="ACHKAU_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFO5533623.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            1722..1961
                     /gene="relB"
                     /locus_tag="ACHKAU_004710"
     CDS             1722..1961
                     /gene="relB"
                     /locus_tag="ACHKAU_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFO5533624.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            1961..2248
                     /gene="relE"
                     /locus_tag="ACHKAU_004711"
     CDS             1961..2248
                     /gene="relE"
                     /locus_tag="ACHKAU_004711"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFO5533625.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            2320..2475
                     /gene="hokD"
                     /locus_tag="ACHKAU_004712"
     CDS             2320..2475
                     /gene="hokD"
                     /locus_tag="ACHKAU_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5533626.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2692..2943
                     /gene="rem"
                     /locus_tag="ACHKAU_004713"
     CDS             2692..2943
                     /gene="rem"
                     /locus_tag="ACHKAU_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO5533627.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            3010..3288
                     /locus_tag="ACHKAU_004714"
     CDS             3010..3288
                     /locus_tag="ACHKAU_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533628.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            3290..4339
                     /gene="ydfU"
                     /locus_tag="ACHKAU_004715"
     CDS             3290..4339
                     /gene="ydfU"
                     /locus_tag="ACHKAU_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5533629.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            4353..5105
                     /gene="ydfT"
                     /locus_tag="ACHKAU_004716"
     CDS             4353..5105
                     /gene="ydfT"
                     /locus_tag="ACHKAU_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5533630.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(5383..5472)
                     /locus_tag="ACHKAU_004717"
     CDS             complement(5383..5472)
                     /locus_tag="ACHKAU_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533631.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(5527..5739)
                     /gene="cspF"
                     /locus_tag="ACHKAU_004718"
     CDS             complement(5527..5739)
                     /gene="cspF"
                     /locus_tag="ACHKAU_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO5533632.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            6040..6255
                     /gene="cspB"
                     /locus_tag="ACHKAU_004719"
     CDS             6040..6255
                     /gene="cspB"
                     /locus_tag="ACHKAU_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO5533633.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIERGAKGPAAANVIITD"
BASE COUNT         1776 a         1367 c         1633 g         1768 t
ORIGIN      
        1 caccattctg aagaggaact gggttatatc ttcagcctac aggcgaagcg cgttctgtct
       61 aaaaatctca ctttccagta caaaagcagt gcgtttcagg tacgcagtga gggccgggga
      121 tatcgactta ggcattcggt tgttactgta tgtgagaact ttgacggtga aattaacgtt
      181 ctgtatgacg ggaaagcgct gggctgggaa aagtatgttg atggcccgga gcctatacca
      241 ctggatgatg aaaagagtgt ccatgaacga gtggataatg cccgtattga tttacgctca
      301 aaatactatg ttaaatctaa agctgaccat ccctggctta cgcgccgaac gcaaagtcat
      361 cagcaagtta agcccccgaa gttacctaaa aagaagcctg atcccgataa aatagattga
      421 aaccaagatc gattcggttg agtgcatatc cattcatagg gtagattctt aagtcgcgtt
      481 tctgatgttc attttcgggt ggtttgttac ttgttttacc ggggatatgc cagaaacgcg
      541 ctgagtcagt ctgggcggtg cgcgtaatga ggcgttatgg taaatagcct atgctaatgt
      601 ccgctaagag caagaagcgg aagttggcag ttttgtggac tgtccccaca aaagtgacta
      661 cagaaatagt tgcaattcat aattgatcat gggttgtcag ttaaactcgt ggcgatttaa
      721 atagactaat tgggagtgcg tccattactt atatcttgta atgttaacta tcagaaatga
      781 tacaaagata atatgtcttt aaagaaaagg ctgatggcga aaagtggccc gatgagggcc
      841 acaatacggc tgtcacttag acgtaaatat caatggtgcc agcggtattt gtatcgtctt
      901 ttttctcttc ttttttatca ggctgaactg tcgcgtcttt attctttttc tctgcctgct
      961 gccttaacaa ctgctccagt tgagccctga ggctttcaat ttgcttctgt accaatgcag
     1021 ccatttcttt tttctgctgt gtcgtcatcc cctcttccga tgagattttc ccaagctttt
     1081 cagtcagcac ctgaatttgt cttgtgattt tggctatttc tgatgttcct tccggggcgg
     1141 agttgtttga aataacggtt gaggtatttc cctgaattgt gacagacata gatttctcct
     1201 tttaaaaaag cactatcggc atgcacaaaa aaatctttaa tcgtatttct tgtgtcatta
     1261 attgtttgat gttcagattg ttttcctcgc gggctggcgc gcctcagaaa gtaaagcttg
     1321 ttgacagggg taaacgttcg gcaataattt tctgccgcat gcgggtgttg cataaaacgt
     1381 gctacgttcc tttatcgaca ggtcaggtca ccgctcaccc gccgacgaga aagcaacact
     1441 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
     1501 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aacgagctga
     1561 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
     1621 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
     1681 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
     1741 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
     1801 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
     1861 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
     1921 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
     1981 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
     2041 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
     2101 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
     2161 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
     2221 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
     2281 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
     2341 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
     2401 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     2461 gaacctgagg agtaagagac ccggcggggg agaaatccct cgccacctct gatgtggcag
     2521 gcatcctcaa cgcacccgca cttaacccgc ttcggcgggt ttttgttttt attttcaacg
     2581 cgtttgaagt tctggacggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     2641 taagagggat ggtcccttaa aggggagagc taattatccg gaaggattct gatgatgaac
     2701 atcgaagaac tgcgtaaaat tttttgtgaa gatggcctct atgctgtgtg cgttgaaaat
     2761 ggaaatcttg ttagtcatta ccgcattatg tgtttgcgaa agaatggggc tgcgttaatt
     2821 aattttgtgg atgctcgggt cacggacgga tttatcttgc gcgaaggtga gtttgtcact
     2881 tcattacagg cattgaaaga gatcggaata aaagctggct tttctgcttt ttcaggagaa
     2941 taaactcatc tacaatcttg cgcggggctg aactcccgct gagtaacacc gtgccaccgg
     3001 agaaaaccga tggcacgcaa cgcaaaatat tacaattctg ataattcgcc cgttcttgcc
     3061 tgcacgcacg ggcggtattc tcacgcattc aagtctgaat ggttccagca ccctccatgc
     3121 actgcagaac aggccgaatg gctgattcat tcttaccgca ggcgcgggtt cgaggttaag
     3181 aaagctctca gtctcgacta tcggcactgg ataatctctg tcaggctgcc ttattccgaa
     3241 cgcccaccac gtgcgtcccg cactttccag caacggatct ggaggtaacg tgcgggtatt
     3301 acttagacct gttctggtgc ctgagcttgg gctggtggtc cttaagccgg gccgtgaatc
     3361 catacagata tttcataatc ctcgagtgct ggtggaaccg gaaccaaaaa gcatgcgtaa
     3421 tctgccatcc ggagtcgttc ctgccgttcg ccagccgctg gcggaagaca aaacattgct
     3481 gccgtttttt agtaacgaac gggtgattcg tgctgctggc ggcgttggcg cattgtccga
     3541 ctggctatta cgtcatgtta catcctgcca gtggcctaat ggcgattacc atcacactga
     3601 aacagtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact gcgacaacca
     3661 actgcgtgac cagacatcgg aatcactgga gctgcttgct caacaaaatc tgacagcatg
     3721 ggtgattgac gtcatccgtc acgcaataag cggtacgcag gagcgggaat tatctttggc
     3781 tgaattatcc tggtgggcgg tctgcaatca ggtggtggat gcactacctg aggctgtatc
     3841 gcgtcgttcg ctgggattac cagcggaaaa aatctgctcg gtgtaccgcg aaagcgacat
     3901 cgtaccggga gagcagaccg ccaccagcat attgaaacaa cgcacaaaaa atcttgcacc
     3961 gttgccttac gcccaccagc aacaaaaatc accacaggaa aagacggtgg taagcatcac
     4021 cgttgatcca gagtctccgg aatctttcat gaagctgcct aaacgtcgcc gctgggttaa
     4081 ggagaaatac acacgttggg ttaagacaca gccgtgtgct tgctgcggta tgccagccga
     4141 cgatccgcat catctgattg gtcacgggca gggcggaatg ggaacaaaag cacatgatct
     4201 ctttgtgttg cctttgtgca gaaagcatca caacgagctg catacggata cagtggcatt
     4261 tgaagataag tatggctccc aactggagct gatatttcgt tttatcgatc gcgcgctggc
     4321 aattggcgta ctggcgtaag tggagaacga gcatgaacct tgaagcctta ccaaaatatt
     4381 actccccaaa atctccaaaa ttgagcgatg acgctccagc gacaggcacc ggttgtttaa
     4441 caattacgga tgtaatggca gcgcagggga tggtgcagtc gaaagcacca cttgggttgg
     4501 ccttatttct ggcaaaagtt ggtgttcagg accctcagtt tgcgattgaa ggcctgctaa
     4561 attacgcgat ggcactggat aacccgacat tgaacaaatt gagtgaagaa atccggttac
     4621 agattattcc ttacctcgtg aattttgcct ttgctgatta ctccaggtct gcggcaagta
     4681 aggctcgctg tgagcattgt tcaggtacgg gattttataa tgtattgcgc gaagtggtga
     4741 aacactaccg acgcggggaa tctgtaatca aggaagaatg ggtgaaggaa ctatgtcagc
     4801 attgccatgg taagggtgaa gtcagcacag cgtgcagagg gtgtaagggt aaagggattg
     4861 ttctggatga aaaaagaacc cggtttcatg gcgtaccggt atataagatt tgtgggcgtt
     4921 gtaatggaaa ccggtttagt cgtttaccga ccacgctggc acgacgtcat gtccagaagc
     4981 tggtaccaga cctgaccgat tatcagtggt ataaggggta tgcggacgtc attggtaaac
     5041 tggtaacaaa gtgctggcag gaagaagcat acgcggaagc gcaattgagg aaggtgacga
     5101 gataaatgat ttttgctgaa gatggcgaca tgatgtttgc atttttcaaa aaatatggat
     5161 aaaatttttt caacgatggg ctttgtatac ccgacgttaa gaaaaagtag aaaacccgct
     5221 gatgagcggg ttttgtgctt taaatggggc aatggtaatg ttgaatctca tcccgggact
     5281 catgtctgtt aacttattat ttagctggtg acttggttat ttgcctgatg tttaaaatgt
     5341 tttcttccag tacaatgtcc ctaaacacaa tgagtctgct tattatatta ttagcagagc
     5401 tattacggcc aaagtacagc ataagctttt aaagccaatc aaccagtcat caagacagac
     5461 ggggttattc ataaaaactc tccatgtgtg atccgatggg gcctgaaatt aaagctttaa
     5521 tatagctcat gaaaggtaaa cattggcagc tgaagggcca cgcagaccat ttatccggca
     5581 aaattccacg cgtaatccgg tggtaatttc ttctgcatcg cggagattga gcgctgaaac
     5641 atgaagctgg acatcgatac gaccatcgga tggggtgata agacccttgc cgcttttgcc
     5701 gtcaaaggtt ttgacaattc ctgtcatttt acgggacaaa aaaattcctt aatactgata
     5761 acttggcgca ctatacacac gttcctgaag aaagctatag ttttttgatg gggttgaaga
     5821 tggctggatg tctaaaataa acattgcttc atatgttcaa ctatgcgtta atgattgcgt
     5881 cggtttgaag aacagacgat atacgaagta gtttactaaa gcagttctca tttcaggtgt
     5941 tattcactta ttccttcttt gagtctctcc aattaagtac gaagtcgttt ctgttatgca
     6001 aaccatttat gccgaaaggc tcaagttaag gaatgtagaa tgtcaaataa aatgactggt
     6061 ttagtaaaat ggtttaacgc tgataaaggt ttcggcttta tttctcctgt tgatggtagt
     6121 aaagatgtgt ttgtgcattt ttctgcgatt cagaatgata attatcgaac cttatttgaa
     6181 ggtcaaaagg ttaccttctc tatagagaga ggtgctaaag gtcctgcagc agcaaatgtc
     6241 atcattactg attaaaattc atcgctcgtc tgtatacgat aacgaagaag gctgatgcct
     6301 gagtagagat acggacagag tagtgaatat tggatctctt taataaaaag taaggaggtc
     6361 caatacatga aacaatggct agcatatttg gcaaaatctt aatcaggaaa agtatgctaa
     6421 ccattgtggt gaagtgcagg tttgctgcat gaatagtttc acagcagaag ctaactgctg
     6481 gcatcgcaaa acaaagtgcg taagtggatg actcccacaa aaagcaccac aatctcaaac
     6541 ccgc
//
LOCUS       DAZFJF010000206         6526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFJF010000206 DAZFJF010000000
VERSION     DAZFJF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.208"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            713..847
                     /locus_tag="ACHKAU_004720"
     CDS             713..847
                     /locus_tag="ACHKAU_004720"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533634.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            1448..2269
                     /locus_tag="ACHKAU_004721"
     CDS             1448..2269
                     /locus_tag="ACHKAU_004721"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5533635.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            2269..3375
                     /locus_tag="ACHKAU_004722"
     CDS             2269..3375
                     /locus_tag="ACHKAU_004722"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5533636.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            3469..5190
                     /gene="cptA"
                     /locus_tag="ACHKAU_004723"
     CDS             3469..5190
                     /gene="cptA"
                     /locus_tag="ACHKAU_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5533637.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            5264..6262
                     /gene="lpxM"
                     /locus_tag="ACHKAU_004724"
                     /gene_synonym="msbB"
     CDS             5264..6262
                     /gene="lpxM"
                     /locus_tag="ACHKAU_004724"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5533638.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            6315..6503
                     /locus_tag="ACHKAU_004725"
                     /pseudo
     CDS             6315..6503
                     /locus_tag="ACHKAU_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1708 a         1399 c         1599 g         1820 t
ORIGIN      
        1 gtctccagga ttcccggggc ggttcatata cccctaattc aactaattgg aggcattacc
       61 atgcattagt gcaagcgcct tgtggattct tttgctaatg gcggaaacat attgtacaat
      121 attgcattta tccctcaata aataaatgag ttatcagatt gcaaaaatct gttatcaaac
      181 taattcaaat aaaaagagat tgatacgacc aacgtacaca aaaattttcg atattcgaac
      241 taaataacag ctcctaatac cgctacactt tagctatcca gtctgcgcct cagaaatgga
      301 cgatgccgtt gaaggactgg tgtatggcaa tgagccgctt tattatcgaa ttcggtgacc
      361 gcttggacgg tcacttcgga gaaaaggcat ttacacagca tcctaaacag gttcagcata
      421 ggtgatatac cgccttttca ttttcttctc cattatttat aagggaggtg tattattatg
      481 caatgttatt ggttttatcc attgtttttt ttagtttatg aatgttcgca aaaaaaaata
      541 aatgaacaat aattttatgt atttaaaata aattaatttt acttctgtaa tggtgtcatg
      601 attagcagct ttttaaacta atatcgactt tcaataaata aggacaggta atggacaaca
      661 aaaatatctg aacccgggtg agattcataa gttgcacagt gctgtcttca aagtgccaca
      721 cccggaacgt aatcactgcc tgctccttat ggggtatctt catggtgtac aagcatcaga
      781 gttattaggt ataaaactgt ctgatattga tttgcaggcc ggtaacctga atatccggcg
      841 gctctaaacg gtttgtcaac tcagcccctc ggctgaaaca ggaggtacag tccattcgtc
      901 gctggctgaa acttcgggat gaatgggtag aagagggata tgaatggtta tttaacctct
      961 atggcaggtc tgctacaggt attactgttg ttctgggggg cactggtgtg gttaatatct
     1021 acgatgcaag agattctcac gatggcccgg tccatcagtg catgcggcgc gatattgtgc
     1081 gagtattttt cagggggagt ggtatccgga tgcagaaaat tccgctactc gactggtttt
     1141 atgcaataaa tcaggatgat aatcttctga ttttttactg aggcggatat ctctcgctgt
     1201 gtttactgaa attgcggtat tctgtttttt ctgaaaaaca gaaaatctga ttaaaaagag
     1261 catatgcgtt aagggaaatt taagttctgg ttgattaact gataaaaata ttaaaaaatc
     1321 cctgttgtcg gtaatctccg gaccattccg ggattacaca ataatatcac catgtgtgat
     1381 atctatggat cctcactgtt gtttacgcga ggatattttc agaatgctta acagacgata
     1441 attttttatg ttgaatgcaa ggcatcttcc cgtactgatg tatcatcatg tcagtcgttg
     1501 tccggggctt gtgacactct cacctgaaac attttgtgaa cagatgaagt ggctcgcaga
     1561 taataactgg cggacggtaa catctgctga gcttgaatat ttttatcagg ggggaacgct
     1621 tccacgaaag agtgtcatgc tcacattcga tgatggttat cttgataact ggctcagagc
     1681 atggccggtc ctgaaggaat ataaccttca tgcacatatt tttctcatta cggggctgat
     1741 tggtaaggga aacgtacgta gcaggcagga gcaggaatat tctcaccgcg actgtgaacg
     1801 gttgattgcc gataaccgtt cagatgaggt catgcttcgc tggtctgaag tccgggaaat
     1861 gcgtgattcc ggactggttg aatttcatgt gcacacccat tcacataaac gctgggacag
     1921 gttgtctgta tcacgtgctg aacagtgccg gctcatgaaa gaagatattc tggtgggaaa
     1981 acaatgtctg acggaaaagc tggggttctg tagttcccat ctgtgctggc ctgaaggata
     2041 ttataatcgg gattatatta atctcgccgg taagcttggt ttctcttatt tatatacaac
     2101 cgaaagaaga atgaactgtc ctgaaaatgg ttcattacgc atagggcgta tcagcaccaa
     2161 agagcgggaa cattccggct ggctgaaacg acgccttttt tattatacaa cgccactttt
     2221 ttcttcagta ctggctcttc ataaggggcc aagattacca gataattaat gatgaaaatt
     2281 ttattcactg aatcttcgtc agatattggg ggccaggagc tgcaggcact ggcccagatg
     2341 actgctcttc aaaagcaggg acattccgtt ttacttgcct gcagggaaaa aagtaaaatt
     2401 gctccggaag ccagaaaaag agggcatgat gtcactttta ttcctttcag gaacagcctt
     2461 caccttcctt ccatcctcag acttcgacgg attatcgggg aatttaaacc tgatctggtc
     2521 atctgccata gtggccatga cagtaatata gcaggactga gccgtctgat atgctgtcac
     2581 cgcttcagta ttgtcagaca aaaaacctac atcacccgta agacccgaac gttttccctg
     2641 aattatctgt gtgatttcat tgttgtgcct agctcagcca tgatggcgca tctgatggcg
     2701 gaaggtgtca ggacaccggt aacggttatt ccaccgggtt tcgactggcc cgcattgcat
     2761 aatgaagcca tgcgaccgtt acccctgcat atccacgcct gggctgcatc agcagacaat
     2821 gttccgctta tcgttcaggt ggggatgcta cgtcccgaaa aaggacatga atttatgctg
     2881 cgtgtattgt atcagctgaa gatggaaggg aaatcatttc gctggctcgt ggtgggcgcc
     2941 gggcgtgaag aatacgaagc acgtctgcgt cagcagacag aacacctggg aatgagcgga
     3001 gatgtgttaa tggcaggtgc ccttttccca gcgttaccgg tctaccggat tgccagtgtg
     3061 gtggtcatgc cttcggaaaa tgaagcgttt ggtatggtac tggctgaagc gtcagtctct
     3121 ggcgtgcccg ttatcgccag tgagacgggc gggatccctg atgtcattca gaagaatgtg
     3181 acagggacgc tgttacccgt gggggatgtg tctgcatgga ccggcgcact gcgcgatttt
     3241 ttatcccgac cggaacgctt ccgaatgatg gcagccagcg ccagagaaga tattgagtac
     3301 cggtttgata ttaacagaac ggcacagatc attgtgtctc tggctagtca ggctaagggg
     3361 aaatgtaaca gataacggcg gatcctcttt tcagcccgaa ttttgttcag cggttttccg
     3421 gcaagtaacg gagtaattta tcacattgtt taaatacggg gacggtcaat gcatttaaat
     3481 accggacaga acaggcctac tttcagctgg agtgcgctgg gatgggcaat tttttatttt
     3541 ggcttttttt ccactctcct gcaggtcatc atcttcagca gtgggtacag cggaacgaat
     3601 ggaatacggg actcactgtt attcagttgt ctgtggttga tcccggtgtt tctctatcct
     3661 gatcggataa aaataattgc agctgttgtc ggtttcattc tctggggcac gtcgctggca
     3721 gcactttgtt attattttct ctatggtcat gaattctctc aaagtgttct tttcgttatg
     3781 tttgaaacga atgccagaga ggctggtgaa tatttcagcc agtatttcag ccttaaactg
     3841 ttgcttatct cgctggtata tactgcggtg tccgtttttc tgtggacacg tctgcgtcct
     3901 gtatatattc ctttgccatg gcggagaatt gtctctttcc tgctgcttta tgctctgctt
     3961 ctgcatccgg ttgttctgaa atcgttaatc agacaggagc cgctgaatga tactctaggc
     4021 aaactggcat cccgaatgga gcctgctgcc ccctggcagt ttgtatccag ttattatcag
     4081 taccatcagc aactgaatgc actgacaacc ttcctgaatg aaaatagcgc actgccacca
     4141 ctgggtaatc tcagggatga atcaggggag agaccacgca cactggtcct ggtgattggt
     4201 gagtcgacac agcgcgaacg catgagctta tacgggtatc tacgtgaaac gacgccggag
     4261 ctggatgcac tgcgtaaaac cgatccgggt cttactgtgt ttaataatgt ggtggcatcg
     4321 cgtccgtaca ccattgaagc attgcaacag gcccttactt tcgccaacga aaagaatcct
     4381 gatctgtatc tgacgcagcc gtcgctgatg aacatgatga agcaggcagg ctataaaaca
     4441 ttctggatta ccaaccagca gacaatcaca gcccgtaaca ccatgctcac tgtattttcg
     4501 cgccagacgg acaggcagta ctacatgaat cagcagcgaa cacaaagtgc gcgtgaatat
     4561 gacactaacg tgctgaagcc gttccgggaa gtgctgaatg accctgcacc aaagaaactg
     4621 atcatcgtgc atctgctggg tacgcacatt aagtataaat accgttatcc ggaaggtcag
     4681 gggcggtttg atggcattac agggcatatt cccactggat taaatgcgaa agagctggaa
     4741 gtgtataacg attacgataa tgccaatctg tttaacgatc atgtggtggc cagtctgata
     4801 aaggacttca gagcaactgc gccggacggc tttctgcttt atttttcaga tcatggtgaa
     4861 gaagtatatg atactccgcc atataaaacg cagggacgga atgaagataa ccccacacgt
     4921 cccatgtaca ctgttccgtt cctgctgtgg acctcggaaa agtggcatgc tgcacatccg
     4981 cgagattttt cgcagtatgt tgaccgcaaa tacagtctgg ctgaactgat ccacacctgg
     5041 tcagatttgg cgggactgac atacgatggt tacgatccga cccgttctct ggtgaatccg
     5101 caattcaggg aaactacccg ctggattgga aatccgtata aaaagaatgg gctcactgat
     5161 ttcgacactc ttccgtatgg tgagccatag tattttctta tcttcggttc cgttctgaag
     5221 gcaggaggat gtgttttcct ctgctgtccg gtggactcca gggatggtac caccttctgc
     5281 tgtactctgt tatcataatg agataagcag gcagataccg gtaaatatga aaaatatcag
     5341 aactgagttt attccccgct ttaaccttac attatgcttc ccccgttact ggatgacgtg
     5401 gacaggtatc ggtattatct gtgtgtttgc aatggtcccc cccgcactga gagatccatt
     5461 gttgggaaaa ttgggcatgc tggttgggcg tctggggaaa agcgcacgtc aacgggcctt
     5521 gattaattta tcgctttgtt ttccggagta cagtgataaa gaaaaggaga atatcgttga
     5581 tgccatgttt gcaacagcct ccatggccgt tgtgcttatg gctgaattag cactgtccgg
     5641 gcctgataaa atttcgcatc gtatacgctg gaatggtctg gagattgtgg agaagatggc
     5701 acagaacaat gaaaaggtca tttttcttgt gccacatgcc tggggagtgg atattcccgc
     5761 aatgctgata gctgcgtcgg gcagaaaaat ggcagcgatg tttcataacc agagaaatcc
     5821 ggtggtggat tatgtctgga acagtgttcg tcgtcgtttc ggaggaaaac tgcacgcacg
     5881 taatgacggt attgcctcct ttgtccgctc agtccggcag ggatactggg gatattatct
     5941 tcctgatcag gatcatggcc cggaattcag tgagtttgca gatttttttg cgacgtataa
     6001 agcaacttta ccggttatcg gacggctcag caggatcagt ggtgcacgga taataccgct
     6061 tttccctgtt tatgacggaa agacccatca tctgactatt catgtcagcc cgccacttgc
     6121 tatacgacag aagagtgatg ctcatattgc gcgacagata aacgaagtgg ttgaaaattt
     6181 cgttcgtcca catcctgagc agtatacgtg gatcctcaag ttgctgaaaa cgcgtaaaga
     6241 aggggaagag gatccttact gaggcaggtt tttcaggata atgagcttac ccagcaatag
     6301 tggacacacg ctaagtgagt aaactctcag tcagaggtgg ctcatatgac aaaaaaacag
     6361 taccaaccag taaaaaaccc cgtaaatagc attcgcctga atttcgcagt gaagccctga
     6421 agcttgctga acgcatcggt gttgctgtcg cagcccgtgt gctcagtctg tatgaatcac
     6481 agcttgtaag cggctcgtca gaactgtatt gatatttact gagcgg
//
LOCUS       DAZFJF010000207         6197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFJF010000207 DAZFJF010000000
VERSION     DAZFJF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.209"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            418..1434
                     /locus_tag="ACHKAU_004726"
     CDS             418..1434
                     /locus_tag="ACHKAU_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533639.1"
                     /translation="MSSVRTDKFIRYGFAVVAIVAILITTITTPNPTPYQYIVYRIVL
                     VLAAGCIGAVLPGFLEVKFNGWLRASGAVAIFAIVFFISPDLLQQNPESASKPVPPTV
                     IDAYAVNSSNRDRYLELFRTGQFGQRDKLRIGCVEWSESSCVAAGNFLRLLSEAGWEI
                     DSDAVFRMQPSVPVEGVSIVSRGDDLVGLPKVPPHMGRWSRSDISYEILTAAFKLMGV
                     PVYFSSDPTLQPGTTGVYFGPKPKLDAPVMQKDENVRRELLRYVGLGMTIQRACSQQP
                     VELCGGEIFSWETSVSSFLRQQRFNSDAVNRWLALPQVNAASATADMKIKLDLLMSFV
                     LGIE"
     gene            2078..4438
                     /locus_tag="ACHKAU_004727"
     CDS             2078..4438
                     /locus_tag="ACHKAU_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFO5533640.1"
                     /translation="MKSMQSKTGSPLVRTEAELESRLTSALNIAFPNIPREDLIEQRH
                     FTVRLGHGTYKIDSAAHWKKYGRADVLIFHRERPLAVIELKREDLTLTHDDYEQAQSY
                     ANQLTPRPPLVVVTNGKDTRVYDSSNGQQWSGGQDASAAVNKMLANSAKLAAADMRWA
                     IEALMGRETNAWVPAVREETARLLIDITDQPGHSEHPFANNLLFPRKITSLVIESAVM
                     GTAFTIIEGDAQSGKSSCLREISLKTESSDLLAVLMLRGSGPGLFQALANLFAAEFEW
                     NLTSNDARNWLRRMSNCTEGPSLMLAIDDVEPGSQMATDLEELAGIRFGNRLVVVLTT
                     YHANALLKNPNGRTPSAIGSRSKVFKTSPMSLDEFKLAQQILSDQRIVFQQGAEYADD
                     YRSPWVLRTIYDDIVRNHQYQKTDLIAYLPPSPGMELIDAAQKSYESQYDLLRYYRVL
                     ARCALADTNSHSVELMFAKANGFVVRYDALSDEARGVVNELKHMGAVRIFRLSGWEDV
                     VVPTVPAAYLLELSDAVCDELVLRAEQDPQDAGAWLGERLDATYLGDMIGAQAIRRMA
                     AKERYFSFGIIQGLLSIEPYKEPIKNGLFTLAMPDNQQVNLKIEDGLAWISKHGDDAK
                     SVLVNLEDQIPKVISKSTSWMILGQLAKLPSAEVGDDDQRIDAYILLSIGRCPFPLIR
                     TNIEGLPYFEHNFGDQGDVLCLEKASIEVATQAMADLFSAPWLYADQWVDTAIATGSI
                     HLLHRLFAALNTVILRRIPVQSDWANEALNQRVSPALKEAIRSLSS"
     gene            4615..5754
                     /locus_tag="ACHKAU_004728"
                     /pseudo
     CDS             4615..5754
                     /locus_tag="ACHKAU_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5751..5906)
                     /locus_tag="ACHKAU_004729"
     CDS             complement(5751..5906)
                     /locus_tag="ACHKAU_004729"
                     /inference="COORDINATES: protein motif:HMM:NF015301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HFO5533641.1"
                     /translation="MTKGNISERDFDILIKLSGIHSEKAILALKKYYVDGVIRKIDPT
                     HVMWTRP"
BASE COUNT         1625 a         1363 c         1507 g         1702 t
ORIGIN      
        1 cttacttggc tttagtcccg gagtgttgcc aggagtatct ttattatctc gtcggcgttg
       61 atctggtttt cctgtcgatt tagcaattgg ttttggacca ggtttaagac ccatgatagt
      121 tatccttttg agatgtaaga gggaagccct caacattggt atattggata ctgagaaatt
      181 tttcaatggg agtgataaac ggccgaatga ttaactcgat attgtgatca acgtctgttt
      241 tttgcacact gtagactgac tcttggagca gaaggttgct ctgagcgagg aatggacagt
      301 tacactcatc accaacgcca atagcctcat tacgcgtata aagctgtatt aatcattggg
      361 gagcagttta atgcgggatt ttcgacttat ggacttgatt caggtgtcaa aattaaaatg
      421 agcagtgtaa ggacagacaa gttcattagg tatggttttg cggttgtggc gatagttgcc
      481 atactcatta ctactatcac aacccccaat cccacacctt atcaatatat agtttaccga
      541 atagttctgg ttctggctgc cggatgtatt ggtgcagttc tgcctggttt ccttgaagta
      601 aaatttaatg ggtggttaag agcatctggc gcagtggcga tcttcgctat agtgtttttc
      661 ataagcccag atttgcttca acaaaatcct gaatctgcaa gtaaacctgt accgcctact
      721 gtgattgatg catatgccgt taatagttcc aatcgcgaca gatacctcga attatttcga
      781 acaggacagt tcggtcagcg ggacaagctg cgcatcggtt gtgttgagtg gagtgaatca
      841 tcgtgcgtgg ctgctggtaa ttttttgcga ttgttaagcg aagctggctg ggaaatcgat
      901 tctgacgctg tattcagaat gcaaccatcc gtgccagttg agggggtatc gattgtaagc
      961 cgtggcgatg atctggtcgg tttgccaaaa gtaccacccc acatgggccg ttggagtcga
     1021 tcagacatta gttatgaaat tttgacagcg gcatttaaat tgatgggagt tccggtttat
     1081 ttttccagtg acccgacatt acaaccaggt actaccggag tgtactttgg accgaaaccg
     1141 aaactggatg caccagtcat gcaaaaagat gagaatgtgc gacgtgagct tctccgctac
     1201 gtcggtttgg gaatgactat tcagcgagcc tgctctcagc agcccgtcga actttgtggg
     1261 ggtgaaatct tttcctggga aacgtcggta tcttcgttcc taaggcagca gagattcaac
     1321 tccgatgccg taaacagatg gcttgccttg ccgcaggtta atgcggcttc ggcaactgca
     1381 gacatgaaaa taaagctaga tttgttaatg tcatttgttc tgggcatcga gtaactatac
     1441 ctcttgcgag gatgattgag cgtctaatgg accagcttag ccgttagcgc tatttgttta
     1501 cgtaagcctg tggaatccaa ctagtgcttc tacgatgcgt tgtttggtga aaaaaaagtg
     1561 ttgaagcact ggttttagac aactaaagcc acgatttgct catgcgtcga aaatgtagtc
     1621 gtctactgtg tagcacggag gcgatattcg tacgcttaca cttgccgata ggccactggg
     1681 gagtgttctt ggcactaagc tgccaggccc gaaggtctgc tatgagcgag gggctgacat
     1741 tcctattggc attcggtgtg agttatcggg ggcggtcatg cggcatactt ccgatacagg
     1801 cgtttccagc tctgcctgtt tcagggttat attaatctgc tttagaatta atcgtggctt
     1861 tagcatctgc acatcgtgct ggagaaacct gagtgaacta aatcatattc cctttcttca
     1921 gctaatcaat tgatagttat gcggaaaaag ttactttgct tgtcaataat ccatttcaca
     1981 tccgttaatc caatgctatg cgtcttcgca ttaatctgga aaggtagtaa gtgaaataaa
     2041 aacttagcca atgactaata tctgaggata ctttgtcatg aaaagtatgc aatcaaaaac
     2101 gggctctcca ctcgttcgaa ctgaagccga gctagaaagc cggcttactt cagcactaaa
     2161 catagcattt cctaacattc ctcgcgaaga tcttatcgaa cagcgccatt ttaccgttcg
     2221 gctaggccat ggaacttaca aaatagatag cgccgcacac tggaaaaaat acgggcgagc
     2281 ggatgttttg atctttcatc gggagagacc acttgcggtt attgaactga aacgtgaaga
     2341 cctgacactg acccatgatg attatgaaca ggctcaaagc tacgctaacc agttaacacc
     2401 acgtccgcct ctcgttgtag ttacgaacgg taaagatacg cgcgtttatg actcaagtaa
     2461 tggacaacag tggtcgggtg gacaggatgc ctcggcagcg gtaaacaaga tgctcgccaa
     2521 ttctgcaaag ttggcggcgg ctgacatgcg ctgggctatt gaagccttga tggggcgaga
     2581 aactaatgct tgggtgccag ctgtgcgaga agaaacagcc cggctgctga ttgatataac
     2641 tgatcagcct gggcattcag aacatccatt tgccaacaac ctactttttc ctcgcaaaat
     2701 cacgtctctt gtaattgaat ctgctgtgat gggtacagct ttcactatca ttgagggtga
     2761 tgcacaaagc ggaaaaagca gttgcctccg tgaaatttcg ctaaaaacgg aaagtagcga
     2821 tcttttggct gtcctgatgt tacgaggttc cggaccagga ctatttcagg ctctggccaa
     2881 tctttttgca gctgaatttg aatggaattt aacctcaaat gatgcacgta actggttaag
     2941 acgaatgtct aactgcactg aaggtccatc gcttatgctt gctatcgacg acgttgaacc
     3001 aggctcccaa atggctactg atcttgagga acttgcgggt atacgttttg gcaacaggct
     3061 ggtggttgtt ctgacgacct atcatgccaa cgcgttactg aaaaatccaa acggacgaac
     3121 tccgtctgca attggtagcc ggtcgaaggt atttaaaaca tcgcctatgt ccctagatga
     3181 atttaagctg gcgcaacaga ttctctcgga ccagcggata gtttttcaac agggagcaga
     3241 atacgccgat gactaccgct cgccttgggt tcttcgcact atttatgatg acattgtgcg
     3301 caatcatcaa taccagaaaa cggatcttat cgcctatctt cctccatcac ccggtatgga
     3361 acttatagat gctgcgcaaa agagctatga gagtcagtat gatcttcttc gttattatcg
     3421 ggttcttgca cgctgcgcac tagctgacac aaactcccac tcagtagaat taatgtttgc
     3481 taaagcgaat ggatttgtcg tgcggtatga cgcactttct gacgaagctc gtggtgtagt
     3541 gaatgagtta aaacatatgg gggcagttcg tatttttcgc ctttcaggat gggaagatgt
     3601 tgtggtgccg acagttcccg cagcttacct tctggaactg tcggatgctg tttgtgatga
     3661 actggtttta cgggcagaac aagaccctca agacgccggg gcttggcttg gcgaacggct
     3721 agacgccact tatcttggag atatgatcgg tgctcaagcc atacgccgta tggcagctaa
     3781 agagagatat ttcagctttg gaattattca gggccttctc agcattgagc catataaaga
     3841 acctataaaa aacggattgt ttactttagc catgccagac aaccaacaag tcaatctaaa
     3901 gattgaggat ggattagcgt ggatttctaa acacggggat gatgcaaaga gtgttctggt
     3961 gaatttagag gatcaaatcc caaaggtcat ttctaaatca accagttgga tgatactcgg
     4021 gcaactagcg aaactgccgt cagcagaggt gggtgacgat gatcagcgta tagacgccta
     4081 tattttactg agcattgggc gctgcccatt tcctctaatt cgcacgaata tagaaggatt
     4141 gccttacttt gagcataatt ttggcgatca gggtgatgtt ctttgcctag aaaaagcgtc
     4201 tattgaagtt gctactcagg cgatggctga tttattttca gccccatggc tctatgcgga
     4261 ccagtgggtg gatacagcta tcgcgaccgg ttctatacat ttactgcatc gtttgtttgc
     4321 tgctttaaac acagtgatat tgcgtagaat acccgtgcaa tccgactggg ctaatgaagc
     4381 acttaatcaa cgtgtctcac cagcccttaa ggaagccata cgatcattat cttcgtgact
     4441 aagcaatggg attttaatac ataacgagca tctgtccacg caagttaaca attgccttgg
     4501 ctcgatattt tggtttctag tgcatatatg ctgatcttct cttcgttgat ctgatcttac
     4561 ccaccaatag tggacacagg actaagtgag taaactctca accagaggtg actcatgaca
     4621 aaaccagtat caatcagcaa gaagccccgt gaacaacatt cgcctgaatt tcgtaacgaa
     4681 gccctgaaac tcgctgaacg catcggtgtg gccgccgcag cccgtgaact cagcctttat
     4741 gaatctcagc tttatgcctg gcgcagtaaa cagcagcaac aaatgagttc gtcagagcgc
     4801 gaaagcgaac tggccgctga aaatgtccgc cttaaacgac aactggcgga gcaggctgag
     4861 gaactggcca tcctccaaaa ggccgcgaca tacttcgcga atcgcctgaa atgaagtatg
     4921 tcttcatcga aaatcatcgg gcagagttct gcatcaaagc gatgtgtcgt gtacttcggg
     4981 ttgcccgcag cggctggtat gtctggctca ggcgtcgtca ccagatgagc cttcgccaac
     5041 agtttcggct cacctgcgat gccgctgtcc ataaggcatt ctttgaggca aagcagcgat
     5101 acggtgctcc ccgccttgct gacgaactgc cggagttcaa tattaaaacc attgccgcca
     5161 gcctgcgtcg tcaggggctg cgggcgaaag ttcagcccgg tcagctaccg tgcacatggc
     5221 ctgcccgtat tggagaatct gctggagcag gacttcagcg ccagaggccc gaaccagaag
     5281 tgggcgggtg acatcacgta cttgcgtacc gatgagggct ggttgtatct cgcagtagtc
     5341 atcgacctgt ggtcacgcgc cgtcattggc tggtcgatgt caccgcgaat gacagcacaa
     5401 ctggcctgtg atgcactaca aatggcgttg tggcggagaa gacgcccgga aagcgtcatt
     5461 gttcatacgg accgtggtgg tcaatactgt tcaggggatt atcaggcgct gctgaagcga
     5521 cacaacctgc gtggcagtat gagtgcgaaa ggctactgtt atgacaatgc ctgtgtggaa
     5581 agcttctttc attcgctgaa ggtggaatgt atccacgggg aacgctttag cagccgggaa
     5641 ataatgcggg caacggtgtt taattatatc gagtgtgatt acaatcgctg gcgtcgtcac
     5701 agtgcctgtg gcggtctcag cccggaacaa tttgaaaacc ataatctcgc ttagggccgt
     5761 gtccacatta cgtgggtagg atcaattttc ctgatgaccc catcgacata gtattttttc
     5821 aaagcaagaa ttgccttctc gctatgtatt cctgatagct tgatcaggat atcaaagtct
     5881 ctttcagata tattcccctt tgtcatgtgt cactccatga atcaaatttc atagtaatca
     5941 atctttcttt gatcgactta tagataataa gatcagaaat gataaaaaag taacgcatat
     6001 ttcttctcgt catgtctact tataaaattc aaatgaacta acctgaacta cttacactgg
     6061 aatatcatta tattggaata attataaaac tgaacaaaat ggtcaatgta atctggttaa
     6121 tttataaata tttacattgc gtgatgatat ttaattccag tgttaagtaa tgtcacatgc
     6181 ctattattgg ttactac
//
LOCUS       DAZFJF010000208         6186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFJF010000208 DAZFJF010000000
VERSION     DAZFJF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.210"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..91)
                     /locus_tag="ACHKAU_004730"
     CDS             complement(<1..91)
                     /locus_tag="ACHKAU_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5533642.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYT"
     gene            complement(368..655)
                     /locus_tag="ACHKAU_004731"
     CDS             complement(368..655)
                     /locus_tag="ACHKAU_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5533643.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(655..846)
                     /locus_tag="ACHKAU_004732"
     CDS             complement(655..846)
                     /locus_tag="ACHKAU_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO5533644.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(874..1275)
                     /locus_tag="ACHKAU_004733"
     CDS             complement(874..1275)
                     /locus_tag="ACHKAU_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5533645.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            1384..1656
                     /locus_tag="ACHKAU_004734"
     CDS             1384..1656
                     /locus_tag="ACHKAU_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5533646.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            1640..2065
                     /locus_tag="ACHKAU_004735"
     CDS             1640..2065
                     /locus_tag="ACHKAU_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5533647.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(2272..2727)
                     /locus_tag="ACHKAU_004736"
     CDS             complement(2272..2727)
                     /locus_tag="ACHKAU_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533648.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            2806..3921
                     /locus_tag="ACHKAU_004737"
     CDS             2806..3921
                     /locus_tag="ACHKAU_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5533649.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSAYALKADSGA
                     VQQVMTARPKQSHQLQQPEADSAIQREADRVVPENTGLSVGRVDYPDVFEQVWREYPL
                     RAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGP
                     DRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            3928..4674
                     /locus_tag="ACHKAU_004738"
     CDS             3928..4674
                     /locus_tag="ACHKAU_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5533650.1"
                     /translation="MKNIAAAGVLERIRRLAPPASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            4696..5466
                     /locus_tag="ACHKAU_004739"
     CDS             4696..5466
                     /locus_tag="ACHKAU_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5533651.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            5482..5895
                     /locus_tag="ACHKAU_004740"
     CDS             5482..5895
                     /locus_tag="ACHKAU_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5533652.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         1670 a         1297 c         1711 g         1508 t
ORIGIN      
        1 tggtatacgt catcgcggtg aacgtaccgt cctggttggg aaacacgccg cataccagag
       61 attcgttgtt gccaagattg agcgtatcca tgttgacctc attccccctt aacgccgggt
      121 cgcggaacta aaaacctgct gcgctgttat acaaagtgtt cccgccgtca tgttcatacg
      181 cctcgggctg gctacttaac ccctgaccac tgcttggtaa ctcgaagtat tgcccggcgt
      241 tctgtggggc ggggtgggtg gttggtgtat gtaatctaca agtaaaaact gttttgtgtc
      301 aacagttttt aattgttgtt ttgggcaaaa aaatccctcg aaagagggag tatgaaaatt
      361 gttcaactca gatagagaag ggaaattgtc gccgagaatg tgtcacgctt acaatctcaa
      421 tgcttgaagc agctactctg tacaggatta tgtagttagg gtgggtcacg atctctctca
      481 atccagaaac ccgttcgctt ggtggatata agtacggatg ctcagataga ggtaataccg
      541 atgtttcaat gcgtattttt agtctacgtg ctgccggtgg gttctccttg gcgatgtaag
      601 ttatgatctg gcgcaaatca tcgcgagcag acggtagcca taaaatgggt aacattactc
      661 gctcttgtta gttacagcaa tttgagcaat aagattttcc atttcagcca ttacctcgtc
      721 atgtggaatt gcggggcgag tgtctgcaag gcttgacgtt actttagtgc gcaaccattc
      781 gttgtaactg ttttcttgtt cggtagtttc gaattctgaa actatcggag aaagggctgt
      841 acccatggca taactcctct tcttgtactg tggtcacgcc cggcggcttt tttgtgccgc
      901 cagccaccta gcaatggttt cttccattga ttttttcttg tctttgattt cttgaagcat
      961 tttttcttgg tcttcctcag gaaacgcact aaaagcttgg agcagttcgc gttggcgagg
     1021 accaattttc atcgtgtcag gggtgaaaat ttgctcacac tcttcaggag gcaataaaaa
     1081 ccaatgcaat ggatgccctg aaacctcaac cagtttatcc aaacttgagg ctttaggtgt
     1141 agccttaccg ctgacccatt gttgaacagt ttgttgtgtc acaccaattc tacgggcaag
     1201 ctcagcctgg ctccatccag tttcctgaag aagcttgctg attctgtaca tagatacttc
     1261 tagggcgctc atcattattc aattttacag gtaaatactg ttaaaagcat cacaataaaa
     1321 aactgttgat tgcatacagt tttttattgt aggctttgct tatagttttt agaggagggc
     1381 aaaatgctag atagcactcg cgaaaaaatt aggcagaaat acactcaggc tgaaataggt
     1441 cgttatatgg gggtcgctca acagactgtt tggcaatggt ttagctttgg cgttccccca
     1501 aagcaggtaa ttccgttatg ccaactaatg aagtgggaag ttaccccgca tgaaatccgc
     1561 ccagatattt atcctaaccc aaccgacggt ttacctgttg gatgtaaggt taacacatca
     1621 aatgcgccgg agttgattca tgaaaatcaa gcatgaacac atccgcatgg cgatgaatgc
     1681 ctgggcgcat ccggacggcg aaaaagtacc ggctgcgaaa attaccaaag cgtatttcga
     1741 gctgggaatg acgttcccgg aactgtatga cgacagccat ccggaagcca tggctcgcaa
     1801 tactcagaaa attttccgct gggtggagaa agacactcct gatgcggtta aaaaaattca
     1861 ggcgttgtta ccagcgatcg aaaaagcgat gccgcctccg ctggtggccc gaatgcgcag
     1921 ccacagttcc gcttattttc gggagttggt agagacgaag gaacggctgg tgaaagatat
     1981 tgatgatttc gttgcatcag cgatcgttct gttcgatcag atgaatcgtg gtggcccggc
     2041 aggaaacact ctggctgtgc attaattggg taataaatat gagtaatgac aaaaaattga
     2101 cactgagcgt ttacgaaaac agtccgcaca tctggcgtgg cggtttatct gatgtggagc
     2161 tggcagagtg gttgatacat aaagctaatg cgctgctctg gcgtttgtca gccagagaac
     2221 agcgcaagga aaccagaata aagctggctg atgcagaagc gtgtgccggg cttattgagg
     2281 attatacaaa tcttggtatt tcttcagcag agagtgatcc cattcagcct ctgagcaggg
     2341 agtcaatcca gcacgctggt tgtatggcac atcttgtaac tgctcgtcaa catgaggtgg
     2401 gtattggatc acttccggtg ggatattcgc tgattccaga gctggttgaa gcaagaaaat
     2461 cagttcagaa aaagagagat gacgcacttc aattattgag agagcactat ggcgcgatac
     2521 cagaatgcga acagcatcga taccctgaag gttatgaatg gatgcagtct ctttttgaag
     2581 ttcgctaatc aatatgtcga gacgaaggta tgtttcggcg cgcagccagg ctctgtaatc
     2641 cgggagcatt tcggggctgt tacaccagcg gtttgttgct gcaacattta atacatgagc
     2701 ctgataaagg cttttcaaaa aatacatgtc gaacctcctc tggttctgtc gattgggaac
     2761 cacagattat atccggagga aggttcggca ccagatgagg tagccatgcg tgattacgca
     2821 aaagtttctc cgcgattctg gctgggagaa acggggagag aacttagaaa ggcgggtgca
     2881 gaagcgcaag ttgttgcttt ttacctgatg acatcccctc acgcaaatat gctgggtttg
     2941 tattacctgc cagttttata ccttgctcat gaaaccgggc ttggtctgga aggggcttca
     3001 aaggggctta aaagggctgt tgaagctggt ttttgtagct atgaccatga tgcagagatg
     3061 gtctgggtcc atgaaatggc agcctggcag gttggggaaa cgttgaagcc tggcgataac
     3121 cgttgtgcag gtgtcaggaa tgagtatgca tcattacctg aaaacgcttt tctgtcagtg
     3181 ttttacgaca gatataaaac ggatttccat ctggatgtga ggcggaataa tagccgaaat
     3241 tcggtaaggg gcttcgaagg ggcttttaag gggcttcgaa gccaagaaca ggaacaggag
     3301 caggagaaag aacaggaaca ggacaaaaac actatggttc atggcaaaaa aaacaccacg
     3361 aaccaggcag gggatgttca gaccgtcaat cctggtcagc cagcaggcac gacaccggaa
     3421 gccgattcag cgtatgcgct gaaagccgat tcgggcgctg tgcagcaggt gatgaccgca
     3481 aggccgaagc aatcacacca actgcagcag cctgaagccg attccgccat tcagcgggaa
     3541 gccgatcggg tagtcccgga aaacaccggg ctgtctgtgg gacgagtgga ttatccggat
     3601 gtgttcgaac aggtctggcg ggagtacccg ttgcgtgccg gagcaaaccc gaagaaatcc
     3661 gcattcagtg cctggaaggc cagattgcgc gagggggtgc caccagagac catgctggat
     3721 ggtgtgaggc gttacgcgag atacctggcg gcgaccggga aagcgggaac ggaatttgtt
     3781 cagcgagcga cgacgttttt tgggccggac cggaattttg aaaacccctg gttgctcccg
     3841 gtaagcggca cgaacaacca gcgttgtgtg aatcatattt ctgaaccgga taccgaaatt
     3901 ccgccgggat tcagggggtg atgtggcatg aaaaacattg cggcagccgg ggttcttgaa
     3961 cgtattcgca gacttgcacc accggcgtcg gttccaccgt accggacggt ggaggagtgg
     4021 cgggaatggc aacttgctga aggacgaaaa cgcagcgagg agattaaccg ccagaatcac
     4081 cagttgcggg tggaaaaaat cctgaatcgt tcgggcatcc agcctctgca cagcaaatgc
     4141 tcgtttgcga attatcaggt gcagaacgac gggcaaaaat acgcgctgag ccaggccaaa
     4201 tccatagctg acgaactgat gaccgggtgc acgaattttg tgttcagcgg taaaaccggc
     4261 accgggaaaa atcaccttgc agcggcgatg ggtaaccggc tgatggcgaa ggggcgcagc
     4321 gtgattatcg tcaccgtgtc tgatgtcatg agcgtgttgc atgacagcta cgacaacggc
     4381 aaatccgggg aaaaattttt acaggagctt tgcagtgttg atttgctggt cctggatgaa
     4441 ataggcgttc agcgggagac gaaaaacgag caggtggtat tacaccagat aattgatcgc
     4501 cggacagcat cactgtgcag tgtcgggatg ttaacaaacc tgaatcatgc cgcaatgagt
     4561 acgcttcttg gtgagaggat tatggaccgc atgaccatga acggtggtcg atgggtgacg
     4621 tttaactggg atagctggcg tccaaatgtc agcaatatga gggttgtgaa gtaattttgt
     4681 ccggaggaaa ttttaatgga aaccgtatct gacgcactga aagcactgaa aaaagcctct
     4741 tcacatgtgg tggcagctcg ccttggaatc agtcgtgaag aggctgtcaa cgagctgtgg
     4801 gaactcaaaa gaaatggcgt cgttgataaa actggtcaca cctggtttct ggctggcgaa
     4861 ggtgaatccc gggtaaccga agagcggcca gtaaaatctg aagcacagga tatgctgacc
     4921 ggggaggtcg aacaaaaagt taccgcagac atgatgattg agtttatcgg tcaggatggg
     4981 gctaaaacgt gtgaggaact ggcgggtaag ttcggtgtca gtactcgcaa ggttgcttcc
     5041 acgctggcgg tggtaaccgc aacggggcgg ctggcacgcg ttaatcagaa cggtaaattt
     5101 cgttactgca tgccgggcga taatttacca gcagagccga aagccgcgct ggtaacggaa
     5161 agtgatggta aggcctttcc tcagccagca ggtgctgcgt taccagtccg ggaagccgca
     5221 acacaggaag aaattaaaac agaaactgtg gcggacattg tgcagccgtt gccatcgttt
     5281 accgaaacgc aagcagatga gctgattttt ccgtcccttc gcagggcaaa cctggcgctg
     5341 cgcagggcga aaagtgatgt tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg
     5401 aacaagcacc gggatattgt tcgacagatt actgattctt cccgccgtgt tgtatcggaa
     5461 aagtgattgc cggaggcgct tatggcaaaa gtatttacac aggaagagcg ggaaaaaatt
     5521 aaagggcagg ttgttgagct agtacgccgg agtgggcgcg agacgttacg gcaactggaa
     5581 gccaagacag gtgcgacaag atatctgatg agcgttcttg ccagagagct ggttgccagt
     5641 ggcgatgtat acaactctgg ttacgggtta ttcccatctg aacaggctcg taaggactgg
     5701 caaaacgccc gcaaaaaact atcgagggca aaactgaaga aaccggttgt ggttgatcct
     5761 gaccttatct ggtcattacc agacggagaa atacgccgct atgacaggca attgaacata
     5821 atttgtagtg agtgccgtaa cagtgaagtg atgcagcgag ttttgatatt ttacacagga
     5881 gtaatgatgg aatagtgaaa ataacatgaa acctttggat tctggtgttt cagtggatgg
     5941 gaaaagagca gatacataga aaatgaatag caataattca cagtctggat gttgtttgta
     6001 tgcttataaa atgatcctag tatgcattgc cggagttgaa tcagctccaa tgaagaaact
     6061 gagaggagta tttagaagat agctaaatgg atgtaattat tagtatctaa aaaatagtat
     6121 ttttgaaatg ggtctaagaa cacaacaatg tacaaatgag aagttatctt ttaatatgtt
     6181 caaaga
//
LOCUS       DAZFJF010000209         6133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFJF010000209 DAZFJF010000000
VERSION     DAZFJF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.211"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(44..430)
                     /gene="ridA"
                     /locus_tag="ACHKAU_004741"
     CDS             complement(44..430)
                     /gene="ridA"
                     /locus_tag="ACHKAU_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO5533653.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(503..964)
                     /gene="pyrI"
                     /locus_tag="ACHKAU_004742"
     CDS             complement(503..964)
                     /gene="pyrI"
                     /locus_tag="ACHKAU_004742"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO5533654.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(977..1912)
                     /gene="pyrB"
                     /locus_tag="ACHKAU_004743"
     CDS             complement(977..1912)
                     /gene="pyrB"
                     /locus_tag="ACHKAU_004743"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO5533655.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1916..2050)
                     /gene="pyrL"
                     /locus_tag="ACHKAU_004744"
     CDS             complement(1916..2050)
                     /gene="pyrL"
                     /locus_tag="ACHKAU_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO5533656.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2331..2726)
                     /locus_tag="ACHKAU_004745"
     CDS             complement(2331..2726)
                     /locus_tag="ACHKAU_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5533657.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(2857..3570)
                     /gene="bdcA"
                     /locus_tag="ACHKAU_004746"
     CDS             complement(2857..3570)
                     /gene="bdcA"
                     /locus_tag="ACHKAU_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5533658.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3641..4234
                     /locus_tag="ACHKAU_004747"
     CDS             3641..4234
                     /locus_tag="ACHKAU_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5533659.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            4379..4831
                     /gene="tabA"
                     /locus_tag="ACHKAU_004748"
     CDS             4379..4831
                     /gene="tabA"
                     /locus_tag="ACHKAU_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO5533660.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4954..>6133
                     /locus_tag="ACHKAU_004749"
     CDS             4954..>6133
                     /locus_tag="ACHKAU_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533661.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINDLKALHDTRIASGTEGNDEINNIKINLSNILI
                     DSLDDAKVNLEKVIDSMLKTFLKLPYINDVKILEWCFNQSMQYSDDTTKIKHARSVID
                     HIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRLILR
                     L"
BASE COUNT         1639 a         1376 c         1474 g         1644 t
ORIGIN      
        1 atcagattta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
       61 tcgcttcgat ctcaattttt acgtctttcg gcagacgggc aacttcaacg caagaacgtg
      121 ccgggaaggt ggcattgtgt tcggtgaaga aggcttcgta ggtagcatta acggttgcga
      181 agtcgttcag atctttcaca aacacggtgg ttttaacgat gtcgcccact ttcaggcctg
      241 cggcttcgac gatcgctttt acgttatcca gcgactgacg tgcctgtgca gcgacgtctg
      301 ccggtacttc gcccgttttc ggatttaccg ggatctggcc ggaggtgatg atcatattgc
      361 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
      421 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
      481 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
      541 cacagtattt gcatttgagc gcgatgtcat tggcgcgttt tcgcacggca aagctggaag
      601 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
      661 gctccggcag actggggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
      721 tggcttgcgg cgcatacaat gccagttgat ctacctgatc ttcactcaaa aaggtatttt
      781 cgattttgat cagatctttg cggcccatct cgccagaagg caggttcaga ccaatggtga
      841 tgcgctgatc cgtttcggtc agcttgaaca gactcaacag cttaaaaccg atctgggcgg
      901 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
      961 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
     1021 ggcgagcgaa aatcccgttg cctgcctgct ggaagtacca ggcgtgtggc gttttatcaa
     1081 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
     1141 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
     1201 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
     1261 cttcaataga gctgtgcaga ctccatgcga tccctttttc atcgagcata tccagaatgt
     1321 attgcggcat tgccagcgcg tccggcgcga tgaagtaaaa acggttgcca tcgaacttcg
     1381 ctaacgcctg agtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
     1441 cgtggagatt gtccagacgc ccctgggttt cctgaatagt gaataagtcc agcaaggttt
     1501 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccggaaaact
     1561 cggtggccag gcgcgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
     1621 tgctgataac cgaaatggta tcggccagcg tttcgccctt tttacccagt gatgtattgg
     1681 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catagatgtt tcgaaagaga
     1741 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
     1801 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
     1861 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
     1921 tcctgacgcc tgggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
     1981 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
     2041 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatagcc agaagcggtg
     2101 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattaa aagtcaaaat
     2161 gtatgcattt ttatttgctt tgattctgga ggagaactgt cgttcggtat caataattgc
     2221 agacatttcc tgcttttttc tttttcactt tcacgcaatc agatatgcat tttattccat
     2281 tcatccgact tatagggcgg agtgtgaaaa cgaacggcta acactattgc ttacagctca
     2341 gggatgcgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccaggt aacgcccacc
     2401 gccgtccagt ttggataagg aggggcgcta aatatttcat ttttgaccgt catgatgtct
     2461 tcaaattgtt tttccggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
     2521 catcccgcag cagccagggt cgcatgcaaa ttatcaaatg ccagtctgac ttgttgctga
     2581 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
     2641 tcgccggaac gaatagccgc agaataacga tgctcagcat acagtgaatg tcggccagca
     2701 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
     2761 gatttctctt taccgggagc cgcttttagc ggacgacgtg agtaaacaaa acccagacat
     2821 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
     2881 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
     2941 agcgacctct tccggttgcc catgtctttt gatagccatc aaactatgca acatatcgcg
     3001 cattggcccg ttggcgggat tagcgtcggt atcaattggc cctggctgga cgacgttaat
     3061 ggtgatccca cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
     3121 tttgctggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
     3181 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
     3241 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
     3301 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
     3361 aacgacatca atgacagcgt ctctgtcagc actatctgta aatactgctg tcgctccagt
     3421 ctcttgtgcc agatgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
     3481 cccatcagtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
     3541 gagaactgtc ttacctgtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
     3601 attacaaaac tgttttaact ttctgtcaac aagttttgta atggtcacta aaaaacaatc
     3661 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
     3721 gctttttcac caaaaaggtt tcgatgctgt cagtgttgct gaagttactg attatcttgg
     3781 tattaacccc ccgagcctct acgcagcttt tggcagtaaa gttgggttat ttagccgtgt
     3841 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
     3901 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
     3961 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
     4021 tgatattgcc gttcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
     4081 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
     4141 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgcactggc
     4201 gggggaagcg ataaaaacta ttctcaagga gtgatgctgg ccttgacccg aaaggcggga
     4261 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct agcggaattg
     4321 atcatctttt cgtataacgg tggaaatgaa acattgtttt aattaaggag tggaaaagat
     4381 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
     4441 tgagcatatc aaagcgcacg ttacggcaga aacgccaaag ggtaagcacg atatcgaagg
     4501 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
     4561 ggagtatcat gcccgctatc tcgacattca gattgtgtta aaaggtcagg aaggcatgac
     4621 cttcagcacg caacctgcag gcacgccgga taccgactgg ttagctgata aagacatcgc
     4681 atttttgccg gaaggcgttg atgagaaaac agttatcctt aatgaaggtg attttgttgt
     4741 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
     4801 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
     4861 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaga atttgtaatg
     4921 cttagacgtt cttattaact caaggagtcc gtcatggcaa aaatatcaga tttgaattgt
     4981 tatcaacaca tggcatcagc cgacaatgtt aaaccaaaaa atgaagtctt aaatacctgg
     5041 tgtgtgggaa tgaatgattt tgctcgtaat gccgaggggc aggatagcag aaggaatatt
     5101 ctatctcctg gagcattttt ggagtttttg gcaaaaatat ttaccctggg ttatgtggat
     5161 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gtcctcatct
     5221 tatatcaaaa ataatgatgg cagtgaaata atgaattttg ttatgaataa tcctgaaggt
     5281 gagcgtgcgg attcatctaa ggtgattata gaaatttcac tttctactac cactactatg
     5341 gggactcgtc aaggacatac agccattata tttccacaat ctgatttttt gagttttcgt
     5401 tatgaaggta agtcttttga aagaaaagat gatagttcat tacatctgat tactaacaag
     5461 gttctggcgt gttaccaacg cgaagctaac aagaatatgg cgcttctatt aaataatcat
     5521 cagttgaact ggaaggacgc aattgattat aacgagttat tagaaaagcc attagccagt
     5581 acattagaaa aaataaagaa agaacatctg ctattaatgc ctcatgtatg tgatgacacg
     5641 attccatact tactaggcga aggtggtata cttgaagaaa taaatgatct gaaagcgtta
     5701 catgatacca gaattgctag tggtacagaa ggtaatgatg aaattaataa catcaaaatt
     5761 aacctgtcta atattcttat tgattcctta gatgatgcaa aagttaatct cgaaaaggtc
     5821 atcgattcga tgttgaagac ttttttaaaa ttgccatata ttaatgatgt aaaaattctg
     5881 gagtggtgtt ttaatcaaag tatgcaatat tctgatgata ctacaaagat aaagcatgcc
     5941 cgctccgtga tcgatcatat tgattttaac tgcgatcagt ctaaaatagc tgagacatta
     6001 tttttcaacc tcaataaaga accctataaa aacagccctg agttacagga attgatttgg
     6061 gagaagttgg ttgtatatgt caatgatttt aacttaagca atcaagaaaa atcacgctta
     6121 atactaaggt tat
//
LOCUS       DAZFJF010000210         6117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFJF010000210 DAZFJF010000000
VERSION     DAZFJF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.212"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..834
                     /gene="exuR"
                     /locus_tag="ACHKAU_004750"
     CDS             58..834
                     /gene="exuR"
                     /locus_tag="ACHKAU_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO5533662.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1074..1157
                     /gene="yqiM"
                     /locus_tag="ACHKAU_004751"
     CDS             1074..1157
                     /gene="yqiM"
                     /locus_tag="ACHKAU_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO5533663.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1179..1841
                     /gene="yqjA"
                     /locus_tag="ACHKAU_004752"
     CDS             1179..1841
                     /gene="yqjA"
                     /locus_tag="ACHKAU_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO5533664.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1845..2228
                     /gene="mzrA"
                     /locus_tag="ACHKAU_004753"
     CDS             1845..2228
                     /gene="mzrA"
                     /locus_tag="ACHKAU_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO5533665.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2375..2743
                     /gene="yqjC"
                     /locus_tag="ACHKAU_004754"
     CDS             2375..2743
                     /gene="yqjC"
                     /locus_tag="ACHKAU_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO5533666.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2781..3086
                     /gene="yqjD"
                     /locus_tag="ACHKAU_004755"
     CDS             2781..3086
                     /gene="yqjD"
                     /locus_tag="ACHKAU_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO5533667.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3089..3493
                     /gene="yqjE"
                     /locus_tag="ACHKAU_004756"
     CDS             3089..3493
                     /gene="yqjE"
                     /locus_tag="ACHKAU_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5533668.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3483..3782
                     /gene="yqjK"
                     /locus_tag="ACHKAU_004757"
     CDS             3483..3782
                     /gene="yqjK"
                     /locus_tag="ACHKAU_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO5533669.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3968..4360
                     /gene="yqjF"
                     /locus_tag="ACHKAU_004758"
     CDS             3968..4360
                     /gene="yqjF"
                     /locus_tag="ACHKAU_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5533670.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4430..5416
                     /gene="yqjG"
                     /locus_tag="ACHKAU_004759"
     CDS             4430..5416
                     /gene="yqjG"
                     /locus_tag="ACHKAU_004759"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO5533671.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5709..6074
                     /gene="yhaH"
                     /locus_tag="ACHKAU_004760"
     CDS             5709..6074
                     /gene="yhaH"
                     /locus_tag="ACHKAU_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5533672.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1525 a         1517 c         1544 g         1531 t
ORIGIN      
        1 tacccatgaa taaagtggta tgataacttc gtccgcattc cactccggga gacgcatatg
       61 gaaatcactg aaccacgccg tttgtatcaa caacttgccg ctgacctgaa agagcgcatc
      121 gaacagggcg tctatctggt aggtgataaa ctgcctgcag aacgctttat tgccgatgaa
      181 aagaacgtca gccgtacggt agttcgtgaa gccatcatca tgctggaagt tgaaggctat
      241 gtcgaagtgc gtaaaggttc aggtattcat gtggtttcca accagccgcg ccatcagcag
      301 gcggctgaca ataatatgga attcgccaat tacggtccgt ttgagttgct tcaggctcgc
      361 cagctcatcg aaagtaatat tgccgagttc gcggcaactc aggtaacgaa acaagacatc
      421 atgaaactga tggccattca ggaacaggcg cgcggcgaac aatgcttccg tgattccgag
      481 tgggatttac agttccacat tcaggtagcc ctggcgacgc agaactccgc cctggcggcc
      541 atcgttgaaa aaatgtggac ccaacgtagc cataacccgt actggaaaaa actgcacgaa
      601 cacattgatt cccgtaccgt cgataactgg tgtgatgacc acgatcaaat cctcaaggcg
      661 ctgattcgca aagatcctca tgctgctaag ctggcaatgt ggcagcacct ggaaaacacc
      721 aagatcatgt tattcaacga aaccagcgac gacttcgagt tcaatgccga ccgctatctg
      781 ttcgccgaaa acccggtagt gcatctcgat acggccacca gcggcagtaa atgaaatacc
      841 tgtccgacag gcgctgcgac gcgcctgttt gtctcgtcgg taagcgaaaa gtataaagtg
      901 tcagcctgtg taaatcctct cgccaccctc ccctgcattc agcaaaatca gactccacgg
      961 acatgtaatt ttgataacga acaacgttga cctttgttac aattagattc aatttgaatt
     1021 tatgtttttg aatgctttct tatctcacga tttaacaggg aatagttcag gctgtgttga
     1081 tgtatcaaac ccgcagaaca taccaaaaca gcaataacat tgcggtagtg catcttttaa
     1141 aaccagcgtg gcgttaaccg attcaccagg aataatgaat ggaacttttg acccaattgc
     1201 tgcaagccct gtgggcgcag gattttgaaa ccctggccaa tccatcgatg attggcatgt
     1261 tgtattttgt cttgtttgta attttgttcc ttgaaaacgg cttgcttcca gcggcctttt
     1321 taccgggcga cagtttactg gtattggtcg gcgtgttgat tgcgaaaggc gcgatgggtt
     1381 acccgcaaac gattctgctg ctgaccgttg ccgccagcct cggctgctgg gtcagctata
     1441 ttcaggggcg atggctgggc aatacccgca ccgtacaaaa ctggctatct catttacccg
     1501 cgcattatca tcaacgcgca caccatcttt ttcataaaca cggtttatcg gcgctgttaa
     1561 ttggtcgctt tattgcgttt gtcagaacac tgctgccgac gattgccggg ttatcagggc
     1621 tgaataacgc gcgctttcag tttttcaact ggatgagcgg tctgctgtgg gtattgatcc
     1681 tgacaactct gggttacatg ctcggcaaaa cgccggtatt tttaaagtac gaggaccagc
     1741 tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt gtttggcctg gcaggttctc
     1801 tggtcgtgtt atggaaaaag aaatatggaa atcgggggta agggatgcaa atacctcgca
     1861 tgtcgcttcg ccagctagcc tggtccggca ctgttttact tctggtcggc acgctgttgc
     1921 tggcctggtc cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc gttcatcaag
     1981 gcacaacgat gccagacggt ttttcgatct ggcatcacct tgacgctcat ggcattcctt
     2041 tcaaaagtat cacccccaaa aacgacactc tgttaattac atttgactcc agcgaccaga
     2101 gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca tggctacatc attgcgcagc
     2161 aggacaataa cagtcaggct atgcagtggc tgacccggtt acgggataac tctcatcgct
     2221 tcggataact tcctgttatc cgaaacatat cactcacttt ggtgatttca ccgtaactgt
     2281 ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt gtctggacaa tcgttccttt
     2341 gtaataggtc cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg ctttagctgt
     2401 ttctctcttt gctcttagtg ccggtagtta tgccactacc ctgtgtcagg aaaaggagca
     2461 aaatatcctt aaggagatca gctatgccga aaaacaccaa aaccagaatc gtattgacgg
     2521 tctgaataaa gccctgagtg aagtccgggc caactgttca gatagccagc tgcgtgccga
     2581 tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc aagatttagc
     2641 cgaggcgaag caaaaaggcg atgccgataa gattgccaaa cgcgaacgga aactggcaga
     2701 agcgcaggaa gagctgaaaa agctggaagc gcgcgactac taactcacaa tagtcactac
     2761 ttactcacct ggagaaaact atgtcgaaag aacacactac ggaacatctg cgtgctgagt
     2821 tgaaatccct ttccgatacg ctggaagagg tgcttagctc atctggcgag aagtcgaaag
     2881 aagagttgag taagattcgt agcaaagcgg agcaggcact gaaacagagc cgttatcgcc
     2941 tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg cgtgctgatg
     3001 agtatgtgcg tgaaaatccg tggaccggcg tgggcattgg cgctgcaatc ggtgtagtgc
     3061 tcggcgttct gctgtcgcgt cgttaattat ggcggacact catcacgcgc aagggcccgg
     3121 taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa tggtggaaac
     3181 acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa gctaatctct ttcaactttt
     3241 actgatgctg ggcctgacga tgcttttcgc tgcatttggt cttatgagcc tgatggtgct
     3301 aattatttgg gcggttgatc cgcaatatcg cctgaatgcg atgattgcca ccaccgtggt
     3361 gttgctgcta ctggcactaa ttggcggtat ctggacgtta cgtaaatcgc gtaaatccac
     3421 cttgctgcgc catacacgcc atgagctggc aaacgatcgg caactgctcg aggaggagtc
     3481 ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc cagatccagc
     3541 aacaacggct ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca ggcgcttacg
     3601 atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc gctggttggc agtagcgtga
     3661 tggcgatctg gacgattcgc catcctaata tgctggtccg ctgggccaga cgcggttttg
     3721 gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag cttcgcggtt
     3781 aacttccctc tggccggagc aattccggcc ttatccctca aatttcttga agatttttga
     3841 cagttttcct tgctaacaat catcattcac cacgtttatg attctctcca tcgacagcaa
     3901 cgacgctaat accgcgccct tgcacaaaaa aacaatcagc agcctgagtg gcgcagtgga
     3961 gagtatgatg aaaaaattag aagatgttgg tgtactggta gcgcgcattt taatgccgat
     4021 tctgtttatt accgcaggct ggggaaaaat caccgcttac tctggtaccc aacaatatat
     4081 ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg attctgcttg agttcggtgg
     4141 tggtctggca atcctgttcg gtttcctgac tcgcaccaca gccctgttta ctgcgggctt
     4201 tacgctgctg acggcatttt tatttcacag caactttgct gaaggcgtca actcgctgat
     4261 gttcatgaaa aacctgacaa tttctggcgg attcctgctg ctggcaatta ccggtccggg
     4321 cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta tacttaacga
     4381 taaaaaacga ggaggaagct cctcgttttt gctattggag gagagaaaaa tggggcaact
     4441 gattgacggc gtctggcatg acacctggta cgataccaaa tctaccggcg gtaaatttca
     4501 acgttcagct tccgcatttc gtaactggct cactgccgat ggcgcacctg gccccactgg
     4561 cacaggcggt tttatcgcag agaaagatcg ttatcatctc tatgtttcac tcgcctgccc
     4621 gtgggcgcac cgcacgctga tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt
     4681 ttccgtagtg aacccgctga tgctggaaaa cggctggacc tttgatgaca gttttccggg
     4741 agcaaccgga gatacgctct atcaacatga atttctgtat cagctttatc tccacgccga
     4801 tccacactac agcggacgag ttactgttcc cgtgctgtgg gacaaaaaga accacaccat
     4861 cgtcagcaac gaatcagcag aaatcatccg catgttcaat accgcgtttg atgcgctggg
     4921 cgcaaaagcg ggtgattact acccaccagc cctgcaaacg aaaattgacg aacttaacgg
     4981 ctggatttat gacaccgtta acaacggcgt gtataaagct ggttttgcca ccagccagca
     5041 agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt
     5101 aggccagcat cgttacctga ccggcaacca gctaaccgaa gccgatattc gcctgtggac
     5161 cacgctggtc cgttttgatc cagtgtatgt gacccacttc aagtgtgata agcaccgcat
     5221 cagcgattac cttaatctgt atggcttcct gcgtgatatc taccagatgc cgggaattgc
     5281 cgaaacagtc aatttcgatc acatccgtaa tcattacttc cgcagccata agaccatcaa
     5341 ccctacgggg attatttcaa ttggtccatg gcaggatctc gatgaaccgc atggacgaga
     5401 tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct ctgataatta gctaagtgca
     5461 tgttgttcat gtcggatgcg gcgtgaacgc cttatcaaac ctacaaaaca tttatattca
     5521 atatgttgca atgaccgcgc aggccagata agcgtagcgc atctggcgat tttgcgcttg
     5581 tcatcagtct tagcgcgcct tcacagcact ttattaattc cttgaacgaa tatttactgc
     5641 catttcattc acgtctattc ttaatttgct gctttaaaaa caagtgattg agcaaaattg
     5701 aggcaaaaat ggactggtat ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg
     5761 cgcggcgcaa agagtactgg atgtttattc tggtcaacat catctttacg ttcgtgctgg
     5821 ggctgctgga taaaatgtta ggctggcaac gcgctggtgg cgaaggcatc ctgacgacaa
     5881 tctacggtat tctggtgttt ttaccgtggt gggcagttca gttccgccgc ctgcacgaca
     5941 ccgaccgctc ggcgtggtgg gcactgttgt tcttaatccc gtttatcggc tggcttatta
     6001 tcatcgtctt taactgccag gcgggtacgc caggcgaaaa ccgctttgga ccagatccga
     6061 aactggaacc ttaaaacaaa tctgctcgcc ggatgtctga ctcatccggc ttttatc
//
LOCUS       DAZFJF010000211         5946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFJF010000211 DAZFJF010000000
VERSION     DAZFJF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.213"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2433)
                     /locus_tag="ACHKAU_004761"
     CDS             complement(<1..2433)
                     /locus_tag="ACHKAU_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5533673.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRSSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARE
                     RMRELQQIRDSFR"
     gene            complement(2481..2690)
                     /locus_tag="ACHKAU_004762"
     CDS             complement(2481..2690)
                     /locus_tag="ACHKAU_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5533674.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(2786..3160)
                     /locus_tag="ACHKAU_004763"
     CDS             complement(2786..3160)
                     /locus_tag="ACHKAU_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5533675.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     REVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(3166..3882)
                     /locus_tag="ACHKAU_004764"
     CDS             complement(3166..3882)
                     /locus_tag="ACHKAU_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5533676.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(3948..4292)
                     /locus_tag="ACHKAU_004765"
     CDS             complement(3948..4292)
                     /locus_tag="ACHKAU_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO5533677.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(4289..4735)
                     /locus_tag="ACHKAU_004766"
     CDS             complement(4289..4735)
                     /locus_tag="ACHKAU_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5533678.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4732..5082)
                     /locus_tag="ACHKAU_004767"
     CDS             complement(4732..5082)
                     /locus_tag="ACHKAU_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5533679.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(5093..5419)
                     /locus_tag="ACHKAU_004768"
     CDS             complement(5093..5419)
                     /locus_tag="ACHKAU_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5533680.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(5416..>5946)
                     /locus_tag="ACHKAU_004769"
     CDS             complement(5416..>5946)
                     /locus_tag="ACHKAU_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5533681.1"
                     /translation="VSHIPAPRDGRDYDPEVLKQAVLEAVNALPAPQDGRDATALEVL
                     PAIDDQKSFPRGTYATHLGGLWRAYEKTHGMRGWECLVDGVADIDVSMTDERLFSVVI
                     RQSSGQCTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTGDKPGEAHSS
                     GWTLAAKRGRDAGGGK"
BASE COUNT         1206 a         1982 c         1419 g         1339 t
ORIGIN      
        1 gcggaagctg tcgcggatct gctgcaactc ccgcatgcgt tctctggctc gttccccctg
       61 cccgtacccc agcagttcag catcattcga cgcacgcgca gccgcattct cattcttcag
      121 tgtctcttcc cgggatcgca actgttcccg gattttttgc tggtcaatca gggccgcatt
      181 acgcagcagc tcctgcttct gcatctccgt cagggttttc agttcgccct gcgcagtctg
      241 gtatttcagc ttcgccagct ctgtattctg ccccaccagt gccagttgct ctttctgctg
      301 cttcagcagc cgggaaaaac tgtcttccgc tttttccgtc tctgattttc caccccggga
      361 tttgggttta ttcgcctcgt tattgcgcca ggcttccagg gcattactga tataacgttg
      421 tctcgcctcc tgatacggat cacccacaaa accgaggtca tccgccgcat accccagccg
      481 gacacgctct ttttcttccc ctttcagtct ggacagggcc agctcacgct ctgtttttgt
      541 cagggcactc tgctgtttat catccagagt ggcctgtggc agccgtaacg gcacattcac
      601 cagtccctgc cgctgctgaa gcagttcatt acccagcccc agcagacggt tgaattccgt
      661 atgctgacca ttcataacca gcatggactg gtacacctta ttctgctctg ccgcctgctg
      721 acgaattaac gccacacgac ggtcttccag cccggcaagc acatcctgaa tggactgcgc
      781 tttttcctgc atctgtgcca gacgggactg ctcaacggca agctgctctg ttgcctgagc
      841 aagcccttcc gttacggtct tcaccgaggt cagatggttt atcatgaatc cgtcaccggt
      901 cgtccagccc gggttcgcca gaacatactg atatcctgcg attttttcct gcagggattt
      961 cacccggctg gcctgttcat caatcagccg gttctgctct gtcagcgccg cccgtgttcg
     1021 tccttcatta tctgaggctt caggcaaaga cattgacggc gttttatgcg cgatttcatc
     1081 tatcgtcagt gcatactggc gcgcagactc cctggcctgc tcctgattct ggtacagcgt
     1141 gtaccatgct gcagccccca gcatcaccag tccgggtacg ccaccaacca gccccagcgc
     1201 accgctcatc agacgtgagc ccaccgccgt tgtactgttc agcgcattct gggcggcgct
     1261 tctggcagca atatttctgt tcaggcgttc ctgtgtggcc gccagacggg cctctgctgc
     1321 aatctgcatc tccgtcccgc gggctgccgc cacggcctgc tgagcacggt acacggctgc
     1381 tcttgcccgc gccgtggcaa tctgcgttcc cctgaactgt gcttccgcca gtgcaacttc
     1441 attacgtgca gccgtcacaa gtcctgccgt ggcagacatc gctccggagg ccatattgcc
     1501 aaagtaccgg gcaaccccga cggcaaccag tgcccccacg gctgttgcca cattatcaat
     1561 ctgtccggca acaccgttca gcacgccgga gagcgttttc gtcaccccgc tggcctcatt
     1621 cgcaccgccc acccaggcca taaaggcgtt ttccaccttc gtgatactac tggaaaccgt
     1681 ttccggcatg gccgcatatt catcacgtaa tatccccagc tggctgatta acgcggggac
     1741 cactttatcc gctgtcagtt ttccgtcatc cgccattgcc ttcagatctt tacgggccac
     1801 gcccataccc gcagccagtg cacgtacgat ccggtcacca ctttcattga ccgaattaaa
     1861 ctcctcacca cgcaatacac cctgcgccag cgcctgactg aactgggtga tcaccgagcc
     1921 cgcctctgcc gtactggcac cggagatttt cagccctgtc gaaatggcct ccgtcacctt
     1981 cagcacatca tcagcactgt aaccatattc acgcattgag gcggctgagc gggcaaacag
     2041 ggccgcatta tccgaaaatg cggtgcctgt ccgctggctg atatccatca gcactttctg
     2101 tgatgacgaa aattcatcag atgactgcga cgcctgtttc agacgggcat tcacggaact
     2161 ccactcatcc gccagtgaaa tcaggtgtcc ggtggcaaag gcaccggcaa atgcgccagc
     2221 cattccgaca gcagaactgc gaatttccgt caactggctg ttcagttctg ccagggcacg
     2281 tcgctgctcc cgggctgccg cagcggcctg acgcccgcca ttctgcaggg tccggtaata
     2341 ttcactgccc atacgggacg cccgctggat ctccgactgg aatgactgcg aatttgccga
     2401 aattttgata atcagttcac gtaacgtcgc cattcacctt tctccgggcg taaaaaaacc
     2461 gcctcagcgg ttctcatcat tcatgactgt gctgcaaagc tcagcgcgtc ttccagcgcc
     2521 gcaaacggat ccacctccgg cttatcctca tcctcgcccc agcagagcat ggcgtccttc
     2581 agtgcaacat tcatcccctg tgccccgaaa accgctttca cgatctgtgc attacggata
     2641 tccccgcgct catcacccag cggggacacc ctgtcaaact ccatccacat catcgcctcg
     2701 ctcgcactca ggctgtgccg cagttcggat aaggtgcgcc ccagacggag cgcaagtcgc
     2761 atcagaaagc gaatttccgg gcgggctact tttttctggc cgactctgca tcagcgatca
     2821 gttccagtgc ctgacgcagc aaccgggcat gtaccggacc atagacggcc agcacctgct
     2881 cacggtcgtc cggagtgaac acccgttgca ggtcagtatc acacaggaca tcgcagaaca
     2941 gcgtcacatc cgcttccagg ttacggcggg ttttcgccac caccgacagg gtatcgtcat
     3001 cctctccatc accattgagc acttcccgcc acagatacca ggcctctgcc gaaggctccc
     3061 gcagcaccac gctgacattt ctccattccg gcaccttcac cgttttatga cggaaccccg
     3121 acagtctggc cagcgccagt gttttcagat cttttgccat aagccttatc cgcccgcacc
     3181 attaaccgtt actgtacacg catcagaggt aatgctctgc ggctgttctg cagaatccgt
     3241 tacctcgcag gtataagccc ccttatcacc tgactgcgta ttggctttac tgaaagtgtc
     3301 agtagtctgt ccctctaccg gctgaccatc cttcttccag gcgtgtttat aaggcggcgt
     3361 tcccccgttg acactgactg acattgtcag cagcgcaccg gtattcacgg taagtgtctt
     3421 ctccagattt ttcacaaacg ccagcggtac cacataggac accggtttac ccttcaggcg
     3481 aagtgaaaac gttgcagcca ccacgccgtt ggtaccggat gaccaggtgt gctgacgcac
     3541 ttccgccagg aacttaaagc ccttaccgga cggaaactgc accttaaacg catacaccgt
     3601 gtcattgtca taggcatcac gcagggcgtt ctgggcctga ttcagataaa aattacccga
     3661 catggaaatc tcggacgacg cccccagacc gttgatgttc tcctgctctg tggagcagag
     3721 cgtggtcaca tcaatatcct gtttctgacc ggcggtgaac tggacttcct tgatggtgca
     3781 gtccaggcgc agatattccg ccttatccat agtttcagca gtcgccgggg cagatgaaat
     3841 catcacctgc gtcagctgtg agcgttcata caaagcagac attctgcctc ctgataataa
     3901 aaaacccgca cgcggcgggg tatgggtttt gtagaaaaaa gaaaaagtca caccgtgacc
     3961 tgaaactcca gggttgcacg gtaacagcgg ttttccggaa tatagtcctg catttcactg
     4021 acggatcccg gggccagcag cattatggct tcacgggcgt cctgacgtat ctgacgcgcc
     4081 tgcgtcacag tcccggcata aacgtctatc tgcaccgaca ctgaggactc cgcctgcccg
     4141 cccatcacgt ccgcagacac cgatgaaatc aggctgaaaa ccacccacgg aagcgccacc
     4201 gacggcctgc catccagcag ggggaccaca tacgggtaca cctgcccgcc ggcaagatgc
     4261 gccagatgag gatacaaatc cgcctccgtc atcgtctcag tacctcatca atggcccggt
     4321 tcatccgagc aatcgccact tgcgctgcct gttcactgcg cacatcaaac gccggacgca
     4381 caaacgggtg cggtggcata ttcacggtcc ccatttccac aaaccgccag tagaaagcgt
     4441 tgcgcgggtt atccgccttc atggtgttat cgctgttacc ggtgtccgga ttaacacccc
     4501 ggatatgcac accggattcc atcccgccat cgcgggagcg ccgggaaagg accaccacat
     4561 tgcggcgcag ttttcccctg cgtaccggtg cccgtgacac cacttcttct ttcagcacat
     4621 tcgcacccgc acgggttgcc tcacgcagca cccggttatt ttctgcacca ctgagaagct
     4681 gcaaatcgcg gctgatgtcc tccagccccg aaaaatccag cagggtttcg atcatttttc
     4741 acctcccagc cgacagagaa tttccagacg tccgccggtc gcatccggca cgggcagccc
     4801 gacaacgttc aggatccggt cacgccaggg accactcagc acatgaagtc gtgacgctgc
     4861 cgtgatttcc cgaccggact gaccgcgcac ccagatgcgg atttccgcct gcgccatttc
     4921 cgcaccagac tgcatccgct cccggctgct cctgccacgg atatccgcat gaattttccc
     4981 gcatgacacc cattcttccg tcatttctcc ggcagcatta cgggttaaca ccgggttcag
     5041 aacacttatc atctgtgtca gacgacctgc agatattgcc attcctccct cctcataaca
     5101 ccgtcggaca acgcaaatcg taaatcagca cggacacaga aaacggcagt tccccctgca
     5161 cgaggtcttc ccgctcagca agatccggat tccggtacag catcccggtc agtcgcatgg
     5221 cagccccctt catccgggtt aatgcctcgc ccgggatcag ctcaccgtcc tcacgaatca
     5281 ctttatcccg gctgccctga atgtaggcca gcagcacggc ggtagcctga cgaaccttgt
     5341 ccatcagcat gtcatcatcc gcgtcatggt caacacgcag atgtgccttg atctcttcca
     5401 gtgtcagtaa tgccgtcatt ttccgcctcc tgcatcccgt ccacgttttg cagccagggt
     5461 ccagcctgat gaatgagctt ctccgggttt atcaccggtc atactgttgc agtgccacag
     5521 cgagcccccc cacgtcaccg tatcgccggg gtggtaggtt tcaccggctc tgaacacacc
     5581 gcggtagagc atcaccggca gggaaaatgt tttttccgta cactggccac tgctctgccg
     5641 gatcaccaca gagaacaacc gctcatccgt catgctgacg tcgatatccg ccaccccgtc
     5701 aaccaggcat tcccatcccc gcatcccgtg cgttttttca tacgcccgcc agagtccacc
     5761 caggtgtgtg gcatacgtgc cccggggaaa ggatttttga tcgtcaatag cggggagcac
     5821 ttccagtgcc gtggcatcac gcccgtcctg cggagccgga agggcattca ccgcctccag
     5881 aaccgcctgc ttcagtactt ccggatcgta atcacgacca tcacgcggag cagggatatg
     5941 gcttac
//
LOCUS       DAZFJF010000212         5923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFJF010000212 DAZFJF010000000
VERSION     DAZFJF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.214"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..973
                     /locus_tag="ACHKAU_004770"
     CDS             263..973
                     /locus_tag="ACHKAU_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5533682.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVFYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1015..3666
                     /locus_tag="ACHKAU_004771"
     CDS             1015..3666
                     /locus_tag="ACHKAU_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5533683.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVITVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3680..4210
                     /locus_tag="ACHKAU_004772"
     CDS             3680..4210
                     /locus_tag="ACHKAU_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5533684.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4223..4726
                     /locus_tag="ACHKAU_004773"
     CDS             4223..4726
                     /locus_tag="ACHKAU_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5533685.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4785..5699
                     /locus_tag="ACHKAU_004774"
     CDS             4785..5699
                     /locus_tag="ACHKAU_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5533686.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1735 a         1258 c         1300 g         1630 t
ORIGIN      
        1 ttaatgagaa atggtttgta tttggagcgc tatgaaaaat cacataatat ctaaactatc
       61 aataaacaac agtagttaaa cttactgtta tttaaaatat ttactttgta gcaaggtttt
      121 cctattaaaa gagaaccaac atttattaca aacgcattaa gtctggaaac ccggcgactc
      181 gccaatgtat tagcaacatt aatcgattgt ctgatgtaac ggttttcccg cttatcctct
      241 taacacatgg agaatgcatc agatgcaaac tacgcgtacc ccctactcta tttcctttat
      301 ggcgacagtg ttacttttat tgctctttgc ttgccatagc accgttgcca acgcagctgt
      361 cgcactgggt gcgacgcggg tattttatcc ggcaaaccag aaacaggttt tattgcctgt
      421 cacgaataat gatcctgcca gtgtctatct cattcagtca tggatagaaa atgcaggtga
      481 ccaaaaagac acccagtttg tcattacccc accgcttttt tccatgcaag gtaaaaaaga
      541 gaatacgctg cggataatca acgctactaa tcatcagtta ccaggcgacc gtgaaagcct
      601 gttctgggtg aatgttaaag ctattcctgc tatggaaaaa gaccagaaga atgagaatac
      661 attacagctg gcaattatta gccgcataaa aatgttttat cgccccacga acctggcaat
      721 ggcacctgaa gaagcgcctg caatgcttcg gtttcgtcgc agcggtagca aactcacgct
      781 tattaatcca acgccctatt tcattaccgt aacaaatatg aaagcaggaa atagcaatct
      841 cccaaacacc atggtgccgc caaaaggtga agtatcggtg gatatcacgc atgctgcaac
      901 cggggatatc agttttcaga cgatcaatga ttacggtgca ttaactccgc gtattaaggc
      961 aaccatgcag taagccagtg acctaattaa taataaactc aatcggaatt aataatgaca
     1021 gcctttcgtg ccgcatttaa ggcatatcgt atgcatcagg tgttaataat gcctcgcttt
     1081 gcacggctta ccattgccct tggcttagca actgcggtat ttcccgttga cgcagagtat
     1141 tattttaatc cacggttttt aagcaatgat cttgctgaat ctgtcgattt atccgccttt
     1201 actaagggac gcgaggcacc acctggtaca tatcgggtcg acatttattt gaacgacgaa
     1261 tttatgacta gtcgggatat tacttttatt gcagatgata ataacgcaga actcatccca
     1321 tgcctgagta cagatcttct tgtcagtctt ggtataaaaa aaatagcgtt attagacaat
     1381 aaagaacatt cggcagaaaa acatgtgccg gacaacagcg catgcacacc acttcaggat
     1441 cgtttggccg acgcatcaac tgagtttgat gttggtcagc aacatttgtc actctccgtt
     1501 ccgcaaattt atgttggcag aatggctcgc ggctatgttt ctcctgatct gtgggaagaa
     1561 ggaataaatg ctgggcttct aaactatagt tttaatggta attctattaa taatcgtagt
     1621 aaccataatg caggaaaatc caactatgca tatttgaatt tacagagtgg catcaacatt
     1681 ggtagttggc gactgcgcga taactcaacg tggagttata acagtgggag tagcaattca
     1741 tctgacagca ataaatggca gcatatcaat acgtcggctg aacgtgacat tattccctta
     1801 cgctcacggt taacggtagg tgatagttat accgatggcg atatttttga tagtgtgaac
     1861 tttcgtggcc tcaaaataaa ttcaacagaa gcgatgttgc ccgatagcca acatggtttc
     1921 gctccggtga ttcatggtat tgcccgtgga accgcacaag tgagtgtaaa acagaacgga
     1981 tacgatgttt atcagactac tgttccaccc ggccctttta ctattgatga tatcaactct
     2041 gcggccaatg gtggtgatct gcaagtaacc ataaaagagg cagacggcag tattcagaca
     2101 ttatatgttc cttattcgtc tgttccggtt ctccaacgtg ctggatatac gcgttatgcg
     2161 cttgccatgg gggaatatcg tagtggaaat aacctgcaaa gctctcccaa gttcatacaa
     2221 ggtagcttga tgcatggtct ggaaggaaac tggacacctt atggcggaat gcaaattgca
     2281 gaagattatc aggccttcaa ccttggtatt gggaaagatt taggactttt tggtgccttt
     2341 tctttcgata tcacgcaggc caatacgaca cttgcagatg gcacccgtca cagtgggcaa
     2401 tcggttaaat ccgtctacag caaatccttc taccagacag gaaccaatat ccaggtcgca
     2461 ggatatcgct attctacgca aggtttttat aacttatccg acagtgccta cagtcgaatg
     2521 agtggttaca ccgtcaagcc tcctaccgga gacaccaatg agcagacaca atttattgat
     2581 tattttaatc tgttctacag taagcgtggt caggaacaaa taagcatctc tcagcagctt
     2641 ggaaattacg gtacgacatt tttcagtgcc agtcgccaaa gttactggaa cacgtcacgc
     2701 agcgaccagc aaatatcatt tggattaaat gtgccgtttg gtgatattac gacttcgctg
     2761 aattacagct attccaataa tatatggcaa aacgatcggg atcatttact cgcttttacg
     2821 cttaatgttc ccttcagtca ttggatgcgt acagacagtc agtcggcatt tcgtaattca
     2881 aacgccagtt acagtatgtc aaacgatttg aaaggcggca tgaccaatct atcgggggtt
     2941 tatggcactc tgctgccgga taataacctg aattatagcg ttcaggtcgg taacacccac
     3001 ggaggtaata catcgtctgg caccagtggt tacagttctc ttaattatcg tggagcttat
     3061 ggtaatacta atgtcggtta cagtcggagt ggtgacagca gccagattta ttacggaatg
     3121 agtggtggga ttattgctca tgctgatggc atcacctttg gacagccgct gggcgacaca
     3181 atggttctgg ttaaggctcc tggtgctgat aatgtcaaaa tagagaacca gaccggaatt
     3241 cataccgact ggcgtggcta tgccatatta ccatttgcga cagaatatag agaaaaccgt
     3301 gttgctctta acgcgaattc ccttgcagat aatgttgaac tggatgaaac cgtgatcact
     3361 gtcatcccaa ctcacggtgc tattgccaga gcaacattta atgcacaaat cggcgggaaa
     3421 gtattaatga cgttgaagta cggtaataag agtgttccat tcggtgcaat tgtcacacac
     3481 ggagagaata aaaatggcag cattgtcgca gaaaacggtc aggtttatct gactggactt
     3541 ccacagtcag ggaaattaca ggtttcatgg ggcaaagata aaaactcaaa ctgtattgtc
     3601 gagtacaagc ttcctgaagt ttctcctggt accttactga accagcagac agcaatctgt
     3661 cgctaaggaa caatagatta tgaaatacaa taacattatt ttcctcggtt tatgtctggg
     3721 gttaaccacc tattctgctt tatccgcaga tagcgttatt aaaattagcg ggcgcgtcct
     3781 cgattatggc tgcacagtct catcggattc gcttaatttt accgtagatc tccaaaaaaa
     3841 cagtgccaga caatttccaa cgaccggtag cacaagtcca gccgtccctt ttcagattac
     3901 gttaagtgaa tgcagcaaag ggacaacggg ggttcgggtt gcatttaacg gtattgagga
     3961 tgcagaaaat aatactttgt tgaaactgga tgaaggaagc aatacggcct ccggtttggg
     4021 tatagaaata ttggacgcaa atatgcgtcc ggtgaaactg aatgaccttc atgccgggat
     4081 gcagtggatc ccactggtac cagaacagaa caatattttg ccttactccg ctcgtctgaa
     4141 gtcaactcag aagtccgtca atccgggact ggtgagggct tcggcaacct ttacccttga
     4201 atttcaataa tcagagtcag atatgaaaag acttcacaag aggttcctgt tagctacgtt
     4261 ttgcgcgtta ttcacagcaa ctctccaggc cgccgatgtc actatcactg ttaatggtcg
     4321 ggtagtcgct aaaccctgca ctattcaaac caaagaagct aacgttaatc tcggggatct
     4381 ttatacgcgc aatctgcaac aacctggttc tgcatctggc tggcacaata ttactttgtc
     4441 attaaccgat tgtccggttg aaacaagtgc agtgacggca atcgtgacag gttcaactga
     4501 caatacgggt tattacaaaa atgaaggtac tgccgaaaat attcagatag agctgaggga
     4561 tgaccaggat gctgcgttaa aaaatggcga tagcaaaacg gttattgttg atgagatcac
     4621 tcgtaatgca cagtttccac ttaaggcaag agctatcacg gtgaatggaa acgcaagcca
     4681 gggaacgatc gaggcgctaa tcaatgtgat ctacacctgg caataacgtc gattcgattt
     4741 tcttagcctt ctttttaaat caatttttaa ccttaggata tatcatgggt aaaacaatca
     4801 gtataaaggt cctgttcggt atttatcttc tattaatggc aggcaaagtt tttgccttca
     4861 gttgtaatgt cgatggcggg tcaagtattg gagcaggtac aacctctgtt tatgttaatc
     4921 tcgaccctgt aatacagccg ggccagaatc tggttgtaga cttgtctcag catataagtt
     4981 gctggaatga ttacggcggc tggtacgaca ctgatcatat aaacctggta caaggctcag
     5041 ctttcgcagg ctccctacag tcatataaag gatcgctgta ctggaataat gtgacctacc
     5101 ccttcccgtt gacgacaaat accaacgtat tggatattgg tgataagaca ccgatgccat
     5161 taccgttaaa actctatatt accccagttg gggcggccgg tggagttgtc ataaaagcgg
     5221 gggaagtcat tgcccgtata catatgtata aaattgcaac attaggaagc ggaaatccgc
     5281 gtaattttac gtggaatatt atttcaaata acaatgttgt tatgccaaca ggcggctgca
     5341 ccgttgatag tcgtaatgtc accgtggatc tcccggactt tcctggaagc gcagaaatac
     5401 cccttggcgt atattgctca agcgaacaaa agctctcatt ttatctttcg ggtgccacca
     5461 ctgacagtag ccgacaagtg tttgctaaca cagcacctga tgcaacaaaa gcatcgggag
     5521 tgggtgtcac tctaatgcgt aatggaaaaa tactggctac gggagaaaat gtctccctgg
     5581 ggacagtaaa taaaagcaaa gtacctctgg gtttaagcgc aacctatgga caaacaggaa
     5641 acaaggtttc agccggaacc gtccagtcag tcattggtgt cacttttatc tatgaataat
     5701 tgtgattttg accttagtct atgcgatcca ttcacgaaaa ttgcgcggat aattattttg
     5761 tgaaggctat tagcctacac ctgtaagaaa atccgcgcac aattgaagcc agagtcttat
     5821 tttttatatt gaaaaatatg ttaatttaaa cactatgaga gatgatgctt aacttatcgt
     5881 atttaatcta ttgtttaacg ataatagatt aaaaatagaa aga
//
LOCUS       DAZFJF010000213         5870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFJF010000213 DAZFJF010000000
VERSION     DAZFJF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.215"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..1249
                     /locus_tag="ACHKAU_004775"
     CDS             17..1249
                     /locus_tag="ACHKAU_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5533687.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            1252..2541
                     /locus_tag="ACHKAU_004776"
     CDS             1252..2541
                     /locus_tag="ACHKAU_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5533688.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPNHIPPKNLVDWVGIKNFIMLFKFKIWSDTFLGVALWTFIWAICATIFTFSFGFIL
                     ALALAKKNIRFAKVWRLIFILPYAIPTFVTLLIFRLLLNGIGPVNSFLNSIGMNSVDF
                     LSVPLNAKITVIAVCVWVGAPYFMLLIAGALTNISRDLYEASEVDGATKWQQFKEITL
                     PMVLHQVAPTLVMTFAYNFNNFGAIYLLTEGGPTNPEYRFAGHTDIFITWIYKLTLNF
                     QQYHIASVISIIIFIFLSVIAIWQFRRMKSFKDDVGM"
     gene            2544..3377
                     /locus_tag="ACHKAU_004777"
     CDS             2544..3377
                     /locus_tag="ACHKAU_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO5533689.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            3397..5196
                     /locus_tag="ACHKAU_004778"
     CDS             3397..5196
                     /locus_tag="ACHKAU_004778"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFO5533690.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDYLEDLGINGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSPI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCVASIQWKDVEHPTVIAYQRDNITFFLNNS
                     EDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            5218..>5870
                     /locus_tag="ACHKAU_004779"
     CDS             5218..>5870
                     /locus_tag="ACHKAU_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO5533691.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFGT"
BASE COUNT         1719 a         1028 c         1225 g         1898 t
ORIGIN      
        1 tcacgaggta aatataatga gactttcaaa tatcgtcaca gttataattc ttgcaatttc
       61 cagtacgcta acaccccagg cgatggcaga aaaattgatt cctgaaactg atgctgagtt
      121 attaatctgg tctgatgcca caagcgcgga atatatgaaa tatgcagcaa cggaattcaa
      181 taaagatttt ggctacaagg tcaagtttac atttcgcaat atagcgccaa tggatgcagc
      241 atcaagaatt atgcaggatg ggggtacgac tcgtgtagct gatgtagctg aaattgaaca
      301 tgataccctg gggcggttag tcgttgctgg cggggttatg gaaaacatgg tctcagctga
      361 gcggattaaa aaaacattta ttccaggcgc agtatcggca gctacatata ataacatcag
      421 ctatggtttt cctgtaagtt tcgcaacgct ggcgcttttc tataataagg atttgttaaa
      481 caccgcacca aaaacattcg aagaaatcaa tactttcagt gaaaagttta ataattcatc
      541 cgagcataaa tatgctctgc tatgggatgt acaaaattat tatgtttcac gtatgtttat
      601 taccttgtat ggtgccaacg aattcggtaa aaccggtaac gatcctaaag ctctaggcat
      661 cgcttcatct gaagcgaaga aagggctaga gacaatgaaa cgcttaaaga aagcgaatcc
      721 ttctaatcct cttgatatgg gtaatccaca agttctaaga ggtctgttta atgaaggaaa
      781 agttgctgct gtaatcgacg gaccttggtc catacaaggt tacattgaca gcggaatcaa
      841 ttttggcgtg acacgcatcc caacattaga tggtcatcag cctcgcacat tttcaacagt
      901 acgactggcc gttgtaagct catttaccga atatcctcat gccgctgaat tattcgctga
      961 ctacctgaca acggataaga tgttgatgaa gcgttatgaa atgacgaatc tcatcccacc
     1021 aatcgattca cttatgaaca aaattagcca gacaggtagt gaagctataa aagcaattat
     1081 tgctcaagct aattattctg acgcaatgcc atcaataccg gaaatgtctt atctatggtc
     1141 tccaatgact aatgctattt tggctacctg ggttgagaat aaaacaccgg atgaagtttt
     1201 aaatcatgca caaacaatta ttgaagaaca actttcgctt caggagtaat tgtgaataca
     1261 caacaactga aaatgaagag tgcacctgtg ctacctattt cctgtttgat tatgggcggg
     1321 actcagttat cacgtcatta ttatgtgaaa ggcgggatat tctttgcaat acaagtatgc
     1381 tttttgcttt atctttccga tattgtgcat acattaatag gtttgttcac attaggtgat
     1441 gtcgcgcaaa ttcgaaaagg gttgactgtt attcagggtg acaattctat atttatgctt
     1501 gtagagggcg tgatagcgac cattattgtt ggcttattcg ccactattta catccttaat
     1561 attaaagatg cgcggaactc atcttattgc catctgactt ttaaacaaca gttatataag
     1621 ctctatgagg ataaatttgc atttatagtc ctaacgcccg catttttagc gagtatagca
     1681 ttcattgttt tacctattgt aattacagtt cttgtatcgt ttacaaacta tgctgcgcca
     1741 aatcacatcc caccaaagaa cctggttgat tgggttggga ttaaaaattt cattatgttg
     1801 tttaaattta aaatatggtc tgatacgttt ctaggtgtcg ctctctggac atttatttgg
     1861 gcaatttgcg cgacgatttt taccttcagt tttggcttta ttctggcttt agcactggct
     1921 aaaaaaaata tacgtttcgc aaaagtctgg cgattgatat ttatactgcc atatgccata
     1981 cccacatttg taacgttact tattttccgt cttcttttaa atggaattgg tccggttaac
     2041 tcgtttctga acagtatcgg aatgaattca gttgattttc tgtcggttcc attaaatgca
     2101 aaaataacag taattgccgt ttgtgtttgg gtaggggcac cgtattttat gctattgatc
     2161 gcgggtgcat taacaaacat atcccgagat ttgtatgagg ctagtgaggt cgatggcgca
     2221 actaaatggc aacagtttaa ggaaattaca ttgccaatgg tgttacatca ggttgcgcca
     2281 actctggtaa tgacatttgc atataatttc aataattttg gtgcaattta tcttctgact
     2341 gaaggtggtc cgactaatcc ggaatacaga ttcgctgggc ataccgatat ttttattacc
     2401 tggatttata aactcacatt gaatttccag caatatcata tagcttctgt aatctccatc
     2461 ataatattta tcttcttatc ggttatagca atatggcaat tcagacggat gaaatcattt
     2521 aaagatgatg tagggatgtg acaatggata aatttttacg taaaatatca acgctcttag
     2581 tttatctttt tttaatctgc aactccattc ttgtgcttgg gccagtgata tggacgataa
     2641 tggcatcttt taaaaaaggg aataatctat ttagttcaac atttagtggc atcgagttta
     2701 catttgacca ctacataacg ttgttcacgg atacgccata tatgcagtgg tatttgaaca
     2761 ctttcattct tgctaccgct aacatgttaa tttcactgtt cgttgtgacg atgaccgcct
     2821 acgttttttc aaggtatcga tttcgtggaa agcgtaatac tttaatgagt attcttgtat
     2881 tgcaaatgtt tcctgcattt ttatcaatga ctgcaattta tatcctgcta tcaaaggcca
     2941 atttgataga cacatatact gggttgcttc tggtttatgt aaccggttca ttgcccttca
     3001 tgacgtggtt ggtaaaaggt tattttgatg ctattccaac ttctttggat gaggccgcta
     3061 aaatagatgg agcagggcat ctgactattt ttattcagat aatattgcca ttggctaagc
     3121 cgattctcgt tttcgttgga ttagttttgt tcactggacc atggatggac ttcatcttgc
     3181 caacgttggt gcttcgtagt gaggataaaa tgaccctggc gattggaatt tttagttgga
     3241 ttgcttctaa ttcagcagag aattatacgc tttttgctgc tggggcgctt ttagttgcag
     3301 tacctatcac attgttattt gtattcacac agaagcatat aactactgga cttgttagcg
     3361 gtgccgttaa ggaataattt ttagggatta gtatttatga ttacagttag ttcgttaaaa
     3421 cactcagcaa aaagtgaaga tagctatgcc tatgataacg aaactttgca tgtacggtta
     3481 cgcacattaa gaggagaagt tgataaagtt attctgtgga taggcgatcc atataattgg
     3541 gctgaaggtg gtcttgacgg cggtaacatg gccgggacag aagcgttcgg ctggattggc
     3601 ggcaacgaga tcgagatgga acaagaagct gtcaccgaat ttcatgatca ctggtttgca
     3661 gtgttcaaac ctcaaaaacg ccgttgccgt tacggtttta tactttttgg gaaagaggga
     3721 gagaagtttc tttttggtga gaaaaggtgt gttgatatca gttcaccgga ctgcgaagaa
     3781 cgagagttaa gccgcttaaa taactttttt tgcttccctt atcttaataa aattgacgtt
     3841 ctaaataccc cctcctgggt aaaaaatacg gtatggtacc aaatttttcc agaccgtttc
     3901 tgtaacggca gacctgaaat atccccagaa ggggtagaac cgtggggcag tgcaccgact
     3961 tcattcaact ttatgggtgg ggatctgtgg ggggttattg ataaactcga ttatctggaa
     4021 gatctgggta ttaatgggat ttatttttgt cctattttta cctcagcttc caaccataaa
     4081 tatgacacca tcgatcattt tagtgttgat ccacatttag gtgggaatat tgctttccat
     4141 actttgattg aagaagcgca taagcgtgga ataaaaatca tgttggatgc agtattcaat
     4201 catttaggcg ctgactctcc aatatggctt gacgttgtac gcaatggtgc caattcacgt
     4261 tacgccgatt ggttctggat tcataaattt cctgtttatc ccgatactcc aaaaagtgaa
     4321 tgggatttta aaaactttaa ctatgaaacc tttggcaatg taattgaaat gcccaagcta
     4381 aatactgaga atgaagagtg ccgtgaatac ttacttagta tagttcgcta ttggacgcaa
     4441 aattttgata ttgatggttg gcgtctggat gttgcgaatg aagtggatca tcatttctgg
     4501 cgagatttca gaaaggtgat taaagatatc aaacctgaat gttatatttt gggtgagata
     4561 tggcatgagg gcaccccctg gttgcgtggt gatcagtttg attcattaat gaattatccc
     4621 ttaacatatg ggattattga ttattttgcg ttacaggata caacaaaaca agaatttatg
     4681 acatccgtta cacgttctta tctttgttat cctaaaaata taactgaagt tatgtttaat
     4741 cttctggata gtcatgacac cgcgcgtatt ttaagcgttt gtttgaatga taaacgaaaa
     4801 gttaaactcg cctatttatt tatgttaact caagcgggct ctccttgtat ctactacggt
     4861 agcgaaatag gaattgatgg ttttaaatca atgacgcttg agaataatag aaaatgtatg
     4921 atatgggatg aaaacaaaca agatcttgaa ttacggcaat ttatacgttg gttaatacgt
     4981 ctacgtaaaa aacatccaca atggtgtgtg gcatcaatac aatggaaaga tgttgaacat
     5041 cctacggtga tcgcttatca acgcgataac atcactttct tcttaaataa tagtgaagat
     5101 actgccaact ttatttatga cggaagaagt atggagatct ccggtttcag ttatgaaatt
     5161 gaaggtttac ctgctgctga tctttatgat ttttaaattt caattaggat gtttgttatg
     5221 ataattgatg cacacactca tattggtgac tttgtgaaaa tccgtatgcc agaagatgtt
     5281 tttctggcat ctctggacaa gtataatatt gattttgcat tatgttcttg tggatctgca
     5341 gtagaagttg accatgacca gaacccgatt ccggatgaag atcaagttac acagcatgat
     5401 aataatgaac gtatgctcag acttgttcgt caacattcaa agcgaattgg cgcgtttatg
     5461 tggattaagc ctcgtctgga aagttgtgat caagattttg aagatatgat tgccagcaat
     5521 cgagatatca tttatggcat caaagttcac ccatatcatt caaaaatggc tttcaattca
     5581 gataaagttc aggaatatat acgccttgct caaaaatatg atctcccggt agttagccat
     5641 acttctaatg attatgaatc cagcccacag ttggtttatg agatggcact taaatatcca
     5701 aatgtgaatt ttgttatgtg tcatatgggg ctcgcgacag ataatcagga agcgatcgaa
     5761 ttgattgcaa aacttcctaa tctctatggc gatactgcgt gggttagggc ggatatggtt
     5821 taccagtcga taaaaatctg tggtagtgat aagattttat ttggtaccga
//
LOCUS       DAZFJF010000214         5867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFJF010000214 DAZFJF010000000
VERSION     DAZFJF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.216"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..603
                     /locus_tag="ACHKAU_004780"
     CDS             <1..603
                     /locus_tag="ACHKAU_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533692.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            631..1092
                     /locus_tag="ACHKAU_004781"
     CDS             631..1092
                     /locus_tag="ACHKAU_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO5533693.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1112..5848
                     /locus_tag="ACHKAU_004782"
     CDS             1112..5848
                     /locus_tag="ACHKAU_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFO5533694.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRSEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRYTAADGAEWL
                     YRYDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQDERRLGIEYDHHNRPETLTDVM
                     GREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAETGAEGFRYDRHGNLLA
                     YTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVINPQGESYRYILDN
                     CGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETILYSRDAAGRLAEVITPEGKTQY
                     AYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDEHTVIRCILPPED
                     ERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQG
                     WNAAGQPVSQRAGFFPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYR
                     LDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETDQYAGGHRLK
                     QAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEYRHDAS
                     GRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGFELISQQFSRVRQ
                     AHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPADTGYPD
                     PRGEWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGEQTYRY
                     VPNPLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGKVREALDVHYEDLVRRKLGGIS
                     QEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKSTRTQIKKTIELAEEQGKEAQFW
                     FKYGVSPKVREYIESKGGKVILGMGN"
BASE COUNT         1446 a         1574 c         1800 g         1047 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg atcattatgg ggcgcaccta ccaccaggaa
       61 aaccgcaccc ccggcagcct gccgggaaca aagacgcaga tgaccatccg ctccaaaacg
      121 tacatgggca gcggatttaa tgagctgaag tttgatgatg cgacagggag agaacaggtc
      181 tacatccacg cgcagaagaa catggatacc gaagtgctca acgaccgtac caccaccgta
      241 aaacacgatc accgcgaaac cgtaaaaaat gaccagacgg tcacgatcca ggaaggtaac
      301 cgccttctta cggtggaaaa aggccacaag atcaccggag tactgaaagg gtctttatct
      361 gaggatgtct ttcaggacag aggcacgatt gccggttcgg tgcatgttga cgctgtaaac
      421 aatggtggcg aaggcgacgg tatacaggct tatacggcga ttaaggaaat tttgctggct
      481 gtggaggaaa gcaaaattgc gctgacgccg gatggcattc agctacaggt cggggaatcg
      541 acggtaatca ggctgtcgaa ggatggcatc accatcgtgg gcggttctgt tttcatcaac
      601 tgattcactc agattatcag ggagtatttt atgcagttca cttttaatga aggccacatt
      661 caactgccgt ctcaatggca ggatcagtca atgcaggtcc tggtgtccac agataacagt
      721 ggcatcaacc tggtcattac ccgcgaagcc gttccacagg gaaccctgac accagagctg
      781 taccaggaaa cgctggcgct gtaccagggc aaacttgatg ggtacaccga acatgcctgc
      841 cgggaaatca ctctggcaga ggccccggcc tggctgctgg attattcctg gaacggtccg
      901 gaagatgagg ggaatcaggg gcggattagc cagatagccg tattccagcg gcgtggagat
      961 acgctgctga ccttcacttt cagcacctcc ttatccctga agaacagtca aaagacgatg
     1021 ctgctggagg tgataaagag cttcaccccg ttgccaccgg agaatgacat ccaaaaggac
     1081 cagccgcgct gacataaacg agggataaca catgagtgaa ggaccaggcg ggccacaggg
     1141 agcgaccgca ggcggtacgc tggcaatgcg aatgctgtca cagcaggcga tggtcgccag
     1201 ccagatgaaa cgggcagcca acgacaaagc cattgcacag atgctggcag caaagaagtc
     1261 cggcccacct gccgccaggc tgggcgatga aattcagcat aaaagttttc tgggggcact
     1321 ggcaggggcc gtgctggggg cgatagtgac catcgcagaa ggttgcctga ttatggccgc
     1381 ctgcgccacc ggcccttatg cgctggttct ggtgcctgcg ctgatgtatg ccagctataa
     1441 ggcgagtgat tatgtggagg agaaacagaa ccagcttgaa tcatggataa acagcttttg
     1501 tgacacggac ggcgccatca ataccggttc tgaaaatgta aacattaacg gaaagcccgc
     1561 tgcaagggcc gccgtcaccc ttccccctcc tcccccacct ggagcaatac ctgaaatccc
     1621 acagggggaa ccctcatggg gtgatattgc cactgacctg cttgaatcgg cagcggaaaa
     1681 agcagtacca ctggcgaagg cctgggggaa cgctgttatc accctgacgg aaagcaatgc
     1741 cggttttatg gatcgcgtca gcgccggcgc atcgcttctg tttcccgccg gtccggtatt
     1801 aatggagttt gccaccatgg tgggcgggcg tagcgaaatc aaaaaagatg tggatttccc
     1861 ggaagccggt gaggacacgg cgctctgcga caaggagaac aaaccaccga ggatagccca
     1921 gggcagtagc aacgtcttta tcaacaatca gcctgccgcg cgcaagggcg acaaactgga
     1981 gtgcagtgcg gcgatcgtgg gaggttcgcc ggacgtcttt attggcggtg aacaggtgac
     2041 ctatctggat atccagccgg agttcccgcc gtggcagaga atgatcctgg ggggaataac
     2101 gatagccagc tatcttctgc cgcctgcggg actgctggga aaactgaaga atctggcgag
     2161 actcggcaaa ctgggaaacc tgctggggaa aagcgggaag ctgctgggcg caaagctcgg
     2221 cgcgttgctg gggaaaacag gtaagtcgtt aaaaagtatt gccaataaag tcatcagatg
     2281 ggtaacagat cctgtcgatc cggtaaccgg cgcatactgc gacgaacgta ccgacttcac
     2341 cctgggccag accctccccc tctccttcac ccgtttccac agttctgtac tgccgctgca
     2401 tggcctgacc ggcgtgggct ggagcgactc ctggagcgaa tacgcctggg tgcgtgaaca
     2461 gggaaatcgg gtagatatta tcagccaggg agccacgctg aacttcgcct tcgacggtga
     2521 cagtgatacg gcggttaacc cgtatcacgc ccagtacatt ctgcgccgcc gcgacgatta
     2581 cctggagctg ttcgacaggg atgcactgag cagccgcttc ttttatgacg cctttccggg
     2641 aatgcgtctg cgccacccgg tgactgacga taccagcgat gaccgcctgg cacacagccc
     2701 cgacgaccgg atgtatatgc tgggcgggat gagcgacacc gccagcaacc gcatcacgtt
     2761 tgagcgcgac agccagtacc ggatcacggg tgtcagtcac accgacggga tccggcttaa
     2821 actgacgtac cacgccagcg gctacctgaa agccattcac cgcacggata acggcataca
     2881 gacgctggcg acctacgaac aggatgcgcg ggggcggctg acagaagcgg atgcgcggct
     2941 ggactaccac ctgttttatg agtacgacgc tgcggaccgg atcatccgct ggtccgataa
     3001 cgaccagacg tggagccgtt tcacctacga tgcacagggc cggtgcgtga atgtcaccgg
     3061 ggcggagggc tattacaacg ccacgctgga ctatggtgac ggctgcacca ccgtgacgga
     3121 cggcaagggc actcaccgtt attactatga tcctgacggc aatattctgc gggaagaagc
     3181 gccggacggc agtaccacca cgtatgaatg ggatgaattc catcacctgc tggcccgcca
     3241 ctcccctgcc gggcgggtgg agaagtttga atacaacgcc gcactcggtc agttaagccg
     3301 ttacacggcg gcagacggcg cggagtggct gtaccgctat gatgagcgcg gcctgccggt
     3361 aagcctggtg tcgccacagg gcgaagagac ccggctggca tacaccgctc aggggctgct
     3421 gtcggggata ttccgccagg atgaacggcg tctgggcata gagtacgacc accacaaccg
     3481 gccggaaaca ctcaccgacg tgatgggccg tgaacaccac accgaataca gcggtcacga
     3541 cctgccggtg aagatgcgcg gccccggcgg tcagtcagtg cggttacagt ggcagcagca
     3601 ccataaactg agcggccttg agcgggcaga aaccggcgca gaaggattcc gctatgaccg
     3661 ccacggcaac ctgctggcgt acacggacgg taacggcgtt gtctggacaa tggaatacgg
     3721 cccgttcgat ttgccggtgg cgcgaacgga cggtgaaggc caccgctggc agtaccgcta
     3781 cgataaagac acgctgcagc tcacagaagt cattaacccg cagggcgagt cataccgtta
     3841 tattctggac aactgtggcc gggtgacgga agagcgtgac tggggcggcg tggtctggcg
     3901 ttaccgctat gacgctgatg gcctgtgtac cgccagggtc aacggcctgg aggaaaccat
     3961 cctctacagc cgggacgccg caggccgcct ggcagaagtc atcacgccgg aaggcaaaac
     4021 gcagtatgcg tatgacaaat ccggcaggct gacgggtatc ttcagcccgg acggcacatc
     4081 acagcgcacc ggctatgacg aacgcgggcg ggtgaatgtc accactcagg gccgacgggc
     4141 cattgaatac cactaccccg atgaacacac cgttatccgc tgtatcctgc caccggaaga
     4201 tgaacgcgac agacaccccg atgaatccct gctgaaaacc acgtaccgct acaacgccgc
     4261 cggagaactg acagaggtca ttctgccggg ggatgagacg ctgacgttca gccgtgatga
     4321 ggcgggacgt gaagtgctcc ggcacagtaa ccggggtttt gcctgtgaac agggctggaa
     4381 tgcagccggc cagcctgtca gtcagcgcgc cggatttttt ccggaggaaa ccacatgggg
     4441 cgggctgctc ccctcactgg tacgggagta ccgttacgac agcgcgggca atgtgtcggc
     4501 tgtcaccagc cgggaagatt acggacggga aacccggcgg gagtaccggc tggaccggaa
     4561 cggccaggtc acggcggtga cagcctcagg caccgggctg ggctatggcg aaggcgatga
     4621 gtcctatggc tatgacagtt gtggctacct gaaggcgcag tctgcgggca ggcaccggat
     4681 aagtgaagag actgaccagt atgccggagg ccaccggctg aaacaggccg gaaacacgca
     4741 gtatgactat gacgccgcag gccggatggt cagccgcacc agacaccgtg acggctaccg
     4801 cccggaaaca gagcggttcc ggtgggacag ccgggaccag ctgaccgggt attgcagcgc
     4861 acagggtgag cagtgggaat accgccacga cgccagcggc agacgaacag agaaacgctg
     4921 cgaccggaag aaaatccgct tcacgtacct gtgggacggc gacagtattg cggaaatccg
     4981 ggaataccgc gatgataaac tgtacagcgt acggcacctg gtgtttaacg gctttgagct
     5041 gataagccag cagttcagcc gggtacggca ggcgcatccg tcggtggccc cgcagtgggt
     5101 gacgcggacg aatcatgcgg tgagcgacct gacgggccgc ccgctgatgc tctttaacag
     5161 tgaaggtaaa accgtctggc ggccgggaca gaccagcctg tgggggctgg cactcagcct
     5221 gcccgcagac accggctacc cggacccgcg cggggagtgg gacccggaag ccgaccccgg
     5281 cctgctgtat gcgggacagt ggcaggatgc ggaatcgggg ctgtgctata accggttccg
     5341 gtactacgag ccggaaaccg gaatgtacct ggtgagtgat ccgctggggt tgcagggggg
     5401 tgagcagact taccggtatg tgcctaatcc actggggtat attgatccgc tggggctggc
     5461 aaaaacttct gttcccgcag aaaaaataag cctttcggat aaagcaagag atctttttag
     5521 acaaggaaaa gtaagagaag cattagatgt tcattatgag gatcttgtac gaagaaagct
     5581 tggtggtata tctcaagaaa ttgccgggcg tgaatatgat gttgttactg acaagatcat
     5641 agcgcaggtc aagaggactt atagctctat agataatcca aaaaacttct taagtaagtc
     5701 tactcggaca caaattaaga agacaattga gctggctgaa gaacagggga aagaggctca
     5761 gttttggttt aaatatggag taagccctaa agtaagagaa tatatagaaa gcaaaggtgg
     5821 gaaagttata ttgggtatgg ggaattaaca tgtcaagata tatactt
//
LOCUS       DAZFJF010000215         5824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFJF010000215 DAZFJF010000000
VERSION     DAZFJF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.217"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..288
                     /locus_tag="ACHKAU_004783"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHKAU_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKAU_004784"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKAU_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO5533695.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHKAU_004785"
     CDS             complement(965..1639)
                     /locus_tag="ACHKAU_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO5533696.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKAU_004786"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKAU_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO5533697.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYSSKSHAHNHDQEHSH"
     gene            complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHKAU_004787"
     CDS             complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHKAU_004787"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO5533698.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(3810..4130)
                     /locus_tag="ACHKAU_004788"
     CDS             complement(3810..4130)
                     /locus_tag="ACHKAU_004788"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO5533699.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4139..4441)
                     /locus_tag="ACHKAU_004789"
     CDS             complement(4139..4441)
                     /locus_tag="ACHKAU_004789"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO5533700.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4451..5230)
                     /locus_tag="ACHKAU_004790"
     CDS             complement(4451..5230)
                     /locus_tag="ACHKAU_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO5533701.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5611..>5824
                     /locus_tag="ACHKAU_004791"
     CDS             5611..>5824
                     /locus_tag="ACHKAU_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533702.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFH"
BASE COUNT         1305 a         1528 c         1570 g         1421 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggagta agcgccagct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgcgaatc ggacgaggtg agggagaacg
     2761 cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc ctggcgaatg cgcgattcgg
     2821 caaaggctac gtcctcggcg atatccgggt caagatgatg gcaaaccatc agcatgtcca
     2881 gatgctcatc aatagtgttg acggtatagg gtagcgtcgg attggttgag gaaggcagaa
     2941 tattggggtg cgcgcaggcg gtgataatat ccggagcatg accaccacct gcaccttctg
     3001 tatggaaggt atggatagtg cgcccgccga tggcagtcag agtatcctca acgaatcctg
     3061 attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc catttcgtcg gctacagtca
     3121 gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc gtgaattttg aggccgataa
     3181 ccccggccgc gacctgctca cgaagcgcat ccggattgga gcaattgcct ttacccagca
     3241 acccgatatt gaccggcagg ctgtctgcag cctgcagcat ttgataaatg taccatggtc
     3301 ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc agtaccgcca ccgatcatgg
     3361 tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg gcagatccag tgaatatgcg
     3421 tatcgacgcc acctgcggta acgatcctgc cttctgcggc aataatttcc gtggatacgc
     3481 cgattgggat agtgacgttg ggttgtatat caggattacc ggctttgccg atagcaaata
     3541 tccttccatc tttgacgccg atatcggctt taacgatccc ccagtaatcg atgatcaggg
     3601 cattggtcag caccagatca gcgcagccgg cggagagcat ttgcccctgt cccataccgt
     3661 cgcggattac tttaccgccg ccgaatttga cctcttcgcc gtaggtagtt aaatcatctt
     3721 cgacctcgat ccacagctca gtgtctgcca gacgaatttt atcaccggtg gtagggccga
     3781 acatgtcagc ataggcctag cgtgaaatat tactcatcat ttgcctccag ctcacccatt
     3841 atatctccgc ggaaaccgtg cacgatacgg gcaccgctca gcgccaccag atcaacctca
     3901 cgcttttggc ctggttcgaa gcgcactgca gttccggcgg cgatattgag acgatagcct
     3961 cttgccttct ggcgatcgaa cttcagtgcc gggttaacct ccgcgaagtg ataatgggaa
     4021 ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa tactgcaggt agcccggccg
     4081 atgttaagct ctatatagcc aggtttaacc ttatattctc cagggatcat ctgttacctc
     4141 agattatcgg attatggacg gtaaccagct tggagccgtc cggaaaggtg gcttcgacct
     4201 ggatatcggg gatcatttct ggtatgcctt ccatgacctg ctcgcgactc aggacatgcc
     4261 gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg agcgccctcc attataaaag
     4321 cgctaatcag ggccacggat tcgggatagt taagtttcag gccgcgggcc agacgacgct
     4381 ctgccagcag tgcagcggta aacagtaata gtttgtcttt ttctcttgga gtcagttcca
     4441 tagcgttctc ttaagtcagc cagatccggg gaagtaccgg tgatttgtca gtcagttgcg
     4501 gccgcataaa ttgccagata tcacgcatta cctgctggca acgcagatta tcatcactta
     4561 aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc ggcgtagttt tccagtggtg
     4621 tgagtttttc gcgtatgtct tccagttgcg tttcattccc caggtagaac agcatcgttc
     4681 ccacccatgg tttccgggca acgcaggtaa gttctccatt ggccacgtgc agacgctcaa
     4741 tcaacagcgg agaaccgtcg acccatatct ccagacggtt agcgagtgtt ccgtggctga
     4801 aggtttctcc gatgaccgga cgtccaaggc acagaagatc ccaggccagc agtgtactgg
     4861 aggacgacag atgaaacacg gtatgcaacg tggcgtgagc gccgggaaaa aaaatcgaat
     4921 cctgtggcaa ccactccagc gttgcgttat tgcacagcgt caggttttgc tgaaggtgcg
     4981 cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg catggtgatc agcgtatggc
     5041 aatcagagtc aattattgcg ttgatgatga gttcgtcacc gccgacgata ccaccgggtg
     5101 gatgaagcag acagaggtgg caggtttctt cttcgggata aaaaggacgc tgaacagtga
     5161 gcggaccgac atgatgacgg gtggtgagcg tggttttccc gtgcagacgc tgaaaacgga
     5221 gatccagcat ggcctgccag ccttttttat tttgtggtaa cgcggtactg agcaatctta
     5281 cgcccctgat aaataaagat aaaacgacag taaagagccg gatttccttg ttatataaaa
     5341 gatgatgcct ttccagtgag ttttcgtctg aataatacga atgtttgttt tcgtcatgaa
     5401 gcgttccggc tcccggggct atacatcgcc agaaaagcaa tgcatattgt cgggattatc
     5461 cagagaatca ggcagaaaga tccgacaatg ggctgtgatt atgccatctg gctcagaatg
     5521 atatcaaggt gttatgggga taacttgctg atacaatagc agtatgacag attcattaat
     5581 gcggaaactg ctcactgaaa cagagtgttc atgactgagc actggtcggg tcatgcagat
     5641 gataatttcc ccttttcttg gggaagcgac agtattttca tgcatgcctg tcttcagtca
     5701 tacggggaga gcatctgtga tacagtccat acatttgttg cggacgtcgc tcctgagtca
     5761 ctgactggtc tgcaaacagt ttatctgtac acggcagggc ttctgtgtga tatcgttttt
     5821 cata
//
LOCUS       DAZFJF010000216         5764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFJF010000216 DAZFJF010000000
VERSION     DAZFJF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.218"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..1484
                     /gene="mltD"
                     /locus_tag="ACHKAU_004792"
     CDS             126..1484
                     /gene="mltD"
                     /locus_tag="ACHKAU_004792"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO5533703.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1532..2302)
                     /locus_tag="ACHKAU_004793"
     CDS             complement(1532..2302)
                     /locus_tag="ACHKAU_004793"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5533704.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2380..3180)
                     /locus_tag="ACHKAU_004794"
     CDS             complement(2380..3180)
                     /locus_tag="ACHKAU_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5533705.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3421..4335
                     /gene="yafC"
                     /locus_tag="ACHKAU_004795"
     CDS             3421..4335
                     /gene="yafC"
                     /locus_tag="ACHKAU_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO5533706.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="ACHKAU_004796"
     CDS             complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="ACHKAU_004796"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO5533707.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5298..5374)
                     /locus_tag="ACHKAU_004797"
     tRNA            complement(5298..5374)
                     /locus_tag="ACHKAU_004797"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5338..5340),aa:Asp,seq:gtc)
     gene            complement(5568..5683)
                     /gene="rrf"
                     /locus_tag="ACHKAU_004798"
     rRNA            complement(5568..5683)
                     /gene="rrf"
                     /locus_tag="ACHKAU_004798"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1493 a         1405 c         1420 g         1446 t
ORIGIN      
        1 caacaacctg aagagcgttt tgcatggtta aggtcaaaga aagataggtt ctgataaaac
       61 tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca actattgaca
      121 cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt tgccagagta
      181 ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa
      241 agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca gatggtgact
      301 tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac cggattcgcg
      361 aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact ttacgggcag
      421 agccgtatat gtactggata gcagggcaag ttaaaaaacg taacatgcct atggaactgg
      481 tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc gccaatgccg
      541 caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa cagacccgca
      601 attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaat atgatgcagc
      661 gtctgaacaa gatgtttgac ggcgactggc ttctgaccgt agcggcttat aacagcggcg
      721 aaggtcgagt catgaaggca attaaaacga acaaagcgcg tgggaaatcc acggacttct
      781 ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg gcattgagtg
      841 atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg
      901 ctctggcgcg tgtgcacctg agcagcccgg ttgaaatggc gaaggttgca gatatggcgg
      961 ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc acgctgggcg
     1021 caagtggtcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg cgtgaatctc
     1081 tggcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat acgccgctta
     1141 acagccgtgt ttacaccgta cgctctggcg acacgctttc aagtatcgct tcacgtctcg
     1201 gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaaa ctgaagccag
     1261 gccaaagttt gacgattggc gcaggcagta gcgcacagcg actggcaaac aacagcgata
     1321 gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa cgccacggcg
     1381 tgaacatcaa agatgtgatg cgctggaaca gcgacactgc gaatctgcaa ccaggcgata
     1441 agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc agataataaa
     1501 aaggcaccga ttcccccggt gcctttttta tttatgccgc tttatgtgct tctaccatga
     1561 tgatatcact ggtgaaagag ccgtcattct gcaaggcaaa atacgttctc acctctgtcg
     1621 atgcgctctg ttggtaaatg cgaatagcgt ctattaacgc ttctggcgta cgcattctca
     1681 cgacccatga agaaaattcc agcggtaact tatctgtaat taaattatca actatcagat
     1741 tggcttcatt gattaacgtc aaccactcac cgctggcgta gtttcgtacg tgagaggtat
     1801 cgcgtaatgc ttctaccgtc tgtaaccaga tgtcgcgcac tgggtgaccc ggagacatta
     1861 cgtccatcac aatcagccta ccgccaggtt tcaatatccg gttcacttca cgcagtgctg
     1921 caccaacatc atgccagtga tgggcagaat aacggctgat aacaatatca aatgcgttgt
     1981 cggcaaatgg cagactttcg gcataaccct ggcgggtggt gatatttttc agttgccgga
     2041 cttcggcagc ttgtgccacg acatctagca tgtgggcaga taagtcatac gccaccaccg
     2101 tgctcacgtt ttgcgcagcg acaaagctgg catgccctgc tccgcagccc atatcaagta
     2161 cacttgcacc aggataatca gccagacgca ccgccaggcg ctgtaaatct cgtccggatg
     2221 catgtacggt actggtcaga tattcgctgg cttgagagct aaactgcttt tctacatggt
     2281 catggtggga ttgtattgtc attgcgctgt ccttttgtta tttgagtata aaagggcagc
     2341 gccaaacgtt ggtctgccct gtggcagacc tgacatacct tatttatcag gcttgccggg
     2401 actgaattca acgagtagcg gattgtgatc ggaagcgcgc gtaaccagta cagaagcttc
     2461 gctgacgttc agaccacggt agaaaacaaa atcgagcggg cgaccaaacg cccgacggcg
     2521 ctgatcatcg gtaaaacgca cctggcgcag cgacatttcc cgcgcaaagc gatataacgc
     2581 gttcatcctt ctacggctcc aggcattgaa atctcccgcc ataatgaccg ggccgctgtg
     2641 gtgagctatc tgatcaccaa taggaagtaa ctgcttacta tagacatcca cgcccagact
     2701 gaagttgacg gcgtgtatat taaccaccat caacaggcgg gtgtcaggta atggatagac
     2761 cgtcaccagt gccgacttcg ccagacgcaa aatgggttct cgttcacgta acgggcagca
     2821 atacactgga tgcgccgccg aaagggtcat tacaccggaa ggatgttgtg gcagcacgaa
     2881 agcgggcacc tgatcggcgg caagatagtt agcggtcgca aactgtacta actctggcgt
     2941 tgtctgcgct tcctgcaata acaccagatg tgcatctttg ccgtagttct ttaataccga
     3001 caaccattca gcgcgttgct gtttgtatat gttccacacc aggatccgaa tacgctcttc
     3061 agtacttaac ggttctccag gtggtaatgc ctggccgatg ctcgcaaaag accccggcgg
     3121 taagatcctt tccgcaggtt gtccggcaac atagcgcatg gcataggtgt tttttcgcac
     3181 ttttgacttt gaaacctcta ttacgtcaac ccttccgcac tcgggagggt tctactgtta
     3241 tagggatttt agctcactct ttcaacgagc aattgtatat tgttatgtaa gcaagtgctt
     3301 acacaggtca gaattttgct cattattgag aacattaaat cggatgacat atcacgcatc
     3361 ctgatggttt aatgacaaat aaattcaaaa tatctcttct attttgcaac aggagcaaaa
     3421 atgaaagcca cgtcggaaga actcgccatt tttgtttcgg tcgtagaaag cggcagcttt
     3481 agccgggcag cggaacaatt agggcaagca aactcagcgg taagccgggc agtgaaaaag
     3541 ctggagatga aacttggcgt tagcctgctt aatcggacca cgcgacaact tagcctgacg
     3601 gaagaaggcc agcgttattt tcgtcgcgta cagtcaattt tgcaggagat ggcagcggca
     3661 gaatcagaaa ttatggagac gcgtaataca ccgcgtggac tgttacgcat cgatgccgca
     3721 actccagtgg tgctgcactt tctgatgccg ttaattaagc ctttccgtga acgctatccg
     3781 gaagttactt tatcgctagt ctcctccgaa acgattatta atttgatcga aagaaaagtg
     3841 gatgtcgcga tacgcgctgg tacgttaacg gattccagct tacgtgccag gccgttattt
     3901 aacagttatc gaaaaattat cgcctccccc gattatattt cccgctacgg gaagccagaa
     3961 acgatcgacg atttaaagca acatgtttgc ctgggattca ctgaacccgc ttccctcaat
     4021 acctggccga tagcctgtag tgatggacaa ttacatgagg tgaagtacgg tttgtcatcc
     4081 aatagtgggg aaacactgaa acagctttgc ctgagtggca acgggattgc gtgtttgtcc
     4141 gactacatga tcgacaaaga aatcgttcgc ggagaattgg tggagttaat ggcagataaa
     4201 gtgttgccag tggaaatgcc attcagtgca gtctattaca gcgaccgtgc ggtaagtacg
     4261 cgcatccggg cttttatcga tttccttagc gagcatgtaa aaacagctcc cggaggagct
     4321 gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag gcggtcgttg
     4381 caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt ttgtgcctta
     4441 agattacttt ccaggttttc acgtttagta gaagaaggaa ttactgagta accttccccc
     4501 atagcccacg ccagaatcac ttgtgccgga gtcgcattgt gtttagctgc gatacgagca
     4561 ataacctcat ctttcaaggc tttcccataa gccagcgtca tataggaagt aatatggatg
     4621 ccgtgctgtt tagcccaggc aaccacttta cggttttgca gataaggaga gagttcaatc
     4681 tggttagtag cgatgttttc agcgccaata acagcaatcg ccttttccat caatgggatc
     4741 gtgaagttgg aaataccgat ctcacgcgtc agcccttgtt ttttggcttc cagcagcgcc
     4801 tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat tagcgtcaga
     4861 tcaacataat cggtacgcaa tttttgcagg ctctctttca gacttgggat caatttgtct
     4921 ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg tggcacgcca
     4981 ctttctgcaa tcgcctgacc tactgcggct tcgttatcat agatttgtgc ggtatcaatt
     5041 gcgcgataac caagttcaag cgccgttttc acagatgaaa taacaacgtc gtctttcaga
     5101 cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta atgctatttt
     5161 cgttaactga ggtgattatg ggcttcagac tgttgaggaa aaagagcgta aaatgcagag
     5221 gatttttgcg attctggcaa taatagatat ccagacgaaa aaaaacctcg agcgttaact
     5281 cgaggcttct taataagtgg cggaacggac gggactcgaa cccgcgcccc cctgcgtgac
     5341 aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca accaccggtt
     5401 ttatgaccgg cttactgctt aatttgatgc ctggcagttt atgaatcact tcgtgattca
     5461 cccttcgggc cgttgcttcg caacgttcaa aagctttcgc ttttgtccta ctctcttatc
     5521 gagtagtgaa ctgcctcaaa gatcagtgcc attcttcgta atttgatgcc tggcagttcc
     5581 ctactctcgc atggggagac cccacactac catcggcgct acggcgtttc acttctgagt
     5641 tcggcatggg gtcaggtggg accaccgcgc taaggccgcc aggcaaattc tgttttatca
     5701 gaccgcttct gcgttctgat ttaatctgta tcaggctgaa aatcttctct catccgccaa
     5761 aaca
//
LOCUS       DAZFJF010000217         5643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFJF010000217 DAZFJF010000000
VERSION     DAZFJF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.219"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..265
                     /locus_tag="ACHKAU_004799"
     CDS             38..265
                     /locus_tag="ACHKAU_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5533708.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            262..687
                     /locus_tag="ACHKAU_004800"
     CDS             262..687
                     /locus_tag="ACHKAU_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5533709.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            756..1793
                     /locus_tag="ACHKAU_004801"
     CDS             756..1793
                     /locus_tag="ACHKAU_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO5533710.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAAPGGTASRAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGCAV
                     ETLDCSDWRDD"
     gene            1786..2247
                     /locus_tag="ACHKAU_004802"
     CDS             1786..2247
                     /locus_tag="ACHKAU_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5533711.1"
                     /translation="MTEQQIRQICRRCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRSKGIERQMTEGELKRLVE
                     RQLTKWAKHVGNGLSVPPVRRQLAAPKRPPGPTPIELLKQEYERRKAAGFV"
     gene            2282..2980
                     /locus_tag="ACHKAU_004803"
     CDS             2282..2980
                     /locus_tag="ACHKAU_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5533712.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGQYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            3002..3226
                     /locus_tag="ACHKAU_004804"
     CDS             3002..3226
                     /locus_tag="ACHKAU_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533713.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            3223..3579
                     /locus_tag="ACHKAU_004805"
     CDS             3223..3579
                     /locus_tag="ACHKAU_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFO5533714.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            3612..3764
                     /locus_tag="ACHKAU_004806"
     CDS             3612..3764
                     /locus_tag="ACHKAU_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533715.1"
                     /translation="MAKKHHYFAEGEITSTCGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            3761..4072
                     /locus_tag="ACHKAU_004807"
     CDS             3761..4072
                     /locus_tag="ACHKAU_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533716.1"
                     /translation="MSAIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            4199..4762
                     /locus_tag="ACHKAU_004808"
     CDS             4199..4762
                     /locus_tag="ACHKAU_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533717.1"
                     /translation="MTELTKEWLQYTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            4872..4976
                     /locus_tag="ACHKAU_004809"
     CDS             4872..4976
                     /locus_tag="ACHKAU_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533718.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            5220..5375
                     /gene="hokD"
                     /locus_tag="ACHKAU_004810"
     CDS             5220..5375
                     /gene="hokD"
                     /locus_tag="ACHKAU_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5533719.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            5543..>5643
                     /locus_tag="ACHKAU_004811"
     CDS             5543..>5643
                     /locus_tag="ACHKAU_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533720.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSRTFKSDWFQ"
BASE COUNT         1535 a         1225 c         1625 g         1258 t
ORIGIN      
        1 tcttgtaatc taaaaatact tagatttagg aggtaaaatg cgagttgatg aacttgtcca
       61 gttttttggt tctgttcaga gggttgccga tttttatggg ataacccgcg aagctattta
      121 catgtggcgt aagcgtcctg gtgaaatagt tccgaagggg agggctgcgg aagctgctgc
      181 atactccaag gggaaattat ctttgaaccc agaactttac aaaaagaagg ataccactca
      241 gggcgaagga aagggggatt catgaaaatc aaacatgagc acatccgcat ggcgatcaat
      301 gcctgggcct atccggacgg tgagaaagtt cctgcagctg aaatagcccg gacttatttc
      361 gagctgggga tgacgttccc ggaactgtac gacgacagcc atccggaagc cctggctcgt
      421 aatacccaga aaattttccg ttggctggat aaagacaccc ctgatgctgt tgaaaaaatt
      481 caggctctgt taccggcgat cgaaaaggca atgccgcctc tgctggtggc ccgtatgcgc
      541 agtcacagct ctgaatatta ccgtgagatc gtcgaacgga gggatcggct ggtgaaagat
      601 gtggatgatt ttgtcgcagc ggcgatcgcc tggggcaccc tgactaacag tggtggtcag
      661 cctggtaatg ctgttgtcgt gcattgacca acaatattca tgccggattt attccggatg
      721 ttcgagggta aagttcggta tcagatgagg tgagtatggc taatgcctgg ctcagattgt
      781 ggcatgacat gccaaatgat cccaaatggc gaaccattgc cagggtctca ggacagccaa
      841 tcgcaacagt gatggcggtg tatatccacc ttctggtgag tgcgtcacga aatgtcacga
      901 catgtcacgg cgtgtcacta cgtggtcaca ttgatgtcac gacggaagat ttagcaagtg
      961 cgcttgatgt gacggaagac gtgattgatt caattttgca tgcaatgcag gggcgagttc
     1021 tggatggtga tcttatttcc ggatgggaaa aacgccaggt gatgaaggag gataacggta
     1081 atgtttcgca aaccgcaaaa tccccggcag agcgcaagag agcgcagcgg gagcgggaaa
     1141 gactgcggaa gcagaacact gattgtcacg atgagtcacg acgcgtcacg catatgtcac
     1201 gacaaatcac gacagataca gatacagata aagaattaaa ccccacacat aacgcgcgca
     1261 tgcgcgagag tgctacgacc ggtgagttga atgacgcgtc gttgcagaca gccgaacctg
     1321 aatacctgga cggcctgagc gaaccgatcg ggaaatttcc gatgactgga gtctggcaac
     1381 cgtcgccgga ttttcgacag cgggcagcag tgtggggtat ggctctgcct gagcctgagt
     1441 ttacacctgc tgagcttgcc gcattccggg attactggat ggcggagggg aaggttttca
     1501 cgcaggttca gtgggagcag aaatttgccc gccacgtgca gcacgtcagg gcacaggtaa
     1561 aaccagtcag caagggggta agccatgcag caccaggtgg caccgcatca cgggcagttc
     1621 aggaaatccg ggctgcacgc gaacagtggg aacgtgaaaa cggatttatc agcaacggaa
     1681 acggcctgga agctgtggga gcttatgggg gaggtgtatt cgaaccgctg gactcagaag
     1741 aacggggctg cgccgtcgaa actctggatt gctcagattg gcgcgatgac tgaacagcaa
     1801 atccggcaga tctgccgtcg gtgcatggac cgctgccggg cgggtgaaac gtggcctccg
     1861 gatctggctg agtttgtggc gttgatttcg gagagcgggg caaatccatt tggcctgacg
     1921 gtggatgccg tgatggagga ataccgtcgc tggaggaatg aatcctggcg gtacgacggg
     1981 agtgataaat acccgtggcc acagccggtg ctgtaccaca tttgcctcga gatgcgttca
     2041 aaggggattg agcgccagat gaccgaaggg gagttaaaac ggcttgtgga aaggcagctg
     2101 acgaaatggg caaagcatgt tggtaatggc ctcagtgttc cgccagtccg gcgacaactg
     2161 gcagcaccca aacgcccgcc agggccaacg ccaattgaat tgctgaaaca ggaatatgag
     2221 cgccggaagg cggccggttt tgtctgagtt gagaagtaat ttttaccggg aggaaaattt
     2281 aatggaaacc gtttttgacg cactgaaagc actgaaaaaa gcctcgtcgc acgagattgc
     2341 agcccgtctt gaaatcagcc gtgacgatgc tgttaccgaa ctctggaagc tgaagcgtcg
     2401 tggcgaagct gataacaagg ggtcgatgtg gtggctgacg agtgaggcaa ctgaagcggc
     2461 cccaaaaacc actgcagaga tgctgattaa cgcgattgaa cagcatggtc ctcagtcggc
     2521 tgacgaactg gcgttaatgt tcgggattac ctcccgccgg gcgaattcat cactggcgat
     2581 ggctgtcagc aaaggtcgtc tgattcgcgt aaatcaggac ggtcaatatc gttactgcat
     2641 accgggcgat aatttaccgg cagagccgaa agctgcatcg gtaacggaaa ccgatggtaa
     2701 agcctttcct cagccggcag gtgttgcgtt accagtccgg gaagcggaaa cacaggaaga
     2761 aattaaaact gaaagtgtgg cggtcacagt gcagtcacag tcgtcgttca tcagaaagca
     2821 tccggatggt ctgattttac catcgctgca tgtggctaac cgcgagctgc gccgggcaaa
     2881 aggtcaggtt cagaagtggg agcgcttatg cgccgcgctg cgggaaatta ataagcatcg
     2941 tgatgtcata cagaaaataa cgacaggaga taacgggtaa tttttacagc agggtgatga
     3001 tatgaaaatc agatatcatg atttcggccc cgtgtcacat atgcttattt ccagtacggt
     3061 gctggaaaca agaaaacata atcacatact ggatatgctg cgtcttgctg acccgtatct
     3121 ggtaataaac accagtggaa ttttctttct gagaagcact gtgtcaggaa aaacatcgca
     3181 tgtgcttcgg gcatataaaa cagcagtgcg ggaggaagga gaatgagcga gattaactat
     3241 caggagctgc gtgttgagct gggagcagca aaaaaacgca tagaagagct ggaggctaat
     3301 cgtgtggtgc ttgaggtgga aaatgagagg ttgaaacacg caatggccgt agcccttgag
     3361 catatatccg tcacagatgc aggacaggca ggtgtcgctg caatgattat ctatgatgcc
     3421 atgtaccaca gtgaaaaaac tgacccctac gcttttctgg cagaattgcg ggcgcagggt
     3481 gtggagatgg tgcgcgaaca tccagcaatc aaactttgct ctttgacgca catatgtgac
     3541 gagttagtcg cagagcttcg caaaggaggc aacaagtgag taaaacgaat ccgggctggg
     3601 caaggccact tatggcgaaa aagcatcatt attttgctga aggtgaaata acaagtactt
     3661 gcggtgggtg gatgtatttt ggcaatgagc gtgagccgga tacattcgaa agtccagatg
     3721 attgtaaaaa gtgtcgtaga aaattaaata aggggggtaa atgagcgcaa ttaaagaaat
     3781 gccagtggaa cgtaatgaat atggctgctg gacgcatcct gaatatgaaa aattctgtga
     3841 tggtcgggaa tatatttcaa cggaagagtt taacgcctgg atggagaaaa ataatcttca
     3901 gtgggctatc cgtagtatgg atgaagatga ttttaatctg gacgcagatg gccctgatat
     3961 ttcagcatgg gaacctgagc gaccagaagg tgagggatgg tttatcggtt cgatacatga
     4021 caccgaagat ggtcctgttt gtgtatggct gagaaataag gccgaagcat aaaggctata
     4081 aaccaacaac taaacactga aaatttaaat cagaaatgat ttttattaaa tccttaaccg
     4141 gagggattcc tgcaccctca gaacatcagg agaccgcccg aaagggcggt agtgaaaaat
     4201 gactgaatta accaaagaat ggctacagta cacgattacc ggaattgagt catcacggga
     4261 tgaaataccg ttcggactcg atgaagatca aaacaacatg cttaccgcat taaaaattgc
     4321 actggcatca ctggcatcag tatcggatga acgggcagcc tatgaattat ttatggagaa
     4381 gcgtttcggg gaatctgtag atcgccgcag agcaaaaaat ggcgatagag aatacatggc
     4441 atgggatatg gcgcttggct ggattatctg gtgtcaccgc gccgccatgc ttcaggctgg
     4501 aaactttcgg gaaaataagg gttcgtcaac caataatttt cggataatct cggaaacgtc
     4561 aaccaactct ccggcaatcc ctgatgaggt gttgtccgca atcctgaagg tcgccaggct
     4621 tcgtgcagat ttcgatgctt ctgaagtgga caggcgaggt atcggtagtt gtctggatga
     4681 ggccgagcaa gaacttatcg ttaccattaa cgaatacgcc agtcagatcg cagtagaagc
     4741 gacacagggg gagaaccaat gagccggcca gatgcatttg caggcgttgc tatagctatt
     4801 gcttttctag tatatgttat ttgtcggtgg gggtaaaaac gttcgccggg attcacacca
     4861 agggagggaa tatgtcggat gatatttcac tggtaatgga aggcgctctg gctgttattg
     4921 ctgttgtggg tgtttactgc ctggttgtgt ttttgatgga gcgcctgggg aactgaattc
     4981 attccgtatg ggaattccca tatcgggcaa aacggtttgc tgtaaagcga gagttaagta
     5041 gaattgctgc gggtgcttga ggctgtctgc ctcgggcatg ccaccgtaag gcagacagag
     5101 aaaagcccca gttaacatta cgcgtcctgc aagacgctta acattaatct gaggcccaat
     5161 ctatgcttca caaacgtagg ttagcctctt acgcgccgaa aggcaaggag aagcaggtta
     5221 tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga
     5281 cggcactggt aacgaggaaa gacctctgcg aggtacgaat ccgaaccggc cagacggagg
     5341 tcgctgtctt cacagcttac gaacctgagg agtaagagac caggcggggg agaaatccct
     5401 cgccaccgct gatgtgtcag gcatcctcaa cgcacccgca cttaacccgc ttcggcgggt
     5461 tttgtttttt ctggtcgttc tggtttacaa tccatccgtc agcctgaaca actggcacct
     5521 gctgcgccag cagagaaaac agatggcgca cgataccaaa ttttacaatt cggataactc
     5581 tgccgcccct gccagcaggc acgggcggcg ttcccgcact ttcaaatctg actggttcca
     5641 gca
//
LOCUS       DAZFJF010000218         5612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFJF010000218 DAZFJF010000000
VERSION     DAZFJF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.220"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..680)
                     /locus_tag="ACHKAU_004812"
                     /pseudo
     CDS             complement(1..680)
                     /locus_tag="ACHKAU_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            681..1418
                     /gene="tagH"
                     /locus_tag="ACHKAU_004813"
                     /pseudo
     CDS             681..1418
                     /gene="tagH"
                     /locus_tag="ACHKAU_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
     gene            1415..1939
                     /gene="tssJ"
                     /locus_tag="ACHKAU_004814"
     CDS             1415..1939
                     /gene="tssJ"
                     /locus_tag="ACHKAU_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5533721.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            1942..3272
                     /gene="tssK"
                     /locus_tag="ACHKAU_004815"
                     /pseudo
     CDS             1942..3272
                     /gene="tssK"
                     /locus_tag="ACHKAU_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3277..4038
                     /gene="icmH"
                     /locus_tag="ACHKAU_004816"
     CDS             3277..4038
                     /gene="icmH"
                     /locus_tag="ACHKAU_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO5533722.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4047..>5612
                     /locus_tag="ACHKAU_004817"
     CDS             4047..>5612
                     /locus_tag="ACHKAU_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5533723.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQ"
BASE COUNT         1230 a         1604 c         1592 g         1186 t
ORIGIN      
        1 gacacgcggt ggccttcact ggtatggctg agggtggtta accacccgtg ctcatcatac
       61 tgccactgct ctgccgggtc gccgttcacc gtccggtgcg tgatgcggtc cgacgcatcg
      121 tagtgccaga gggtgataag cccctcgtct tcactctgtg tgagttttct ggtcaggtca
      181 tagtggtaac gttgcgtccg gccgtcaaaa ccgcgctgtt cagtcagcct gtcaaccggg
      241 tcataccgga acgtggactg gctgccgttc tcgttggtca gcacggtgat gcgcccggca
      301 gcgtcatacc ccatgctgcg cgtcagaccg ccttccgtca cactcaccgg acgcccgcgt
      361 ttatcatatg cgatggtgct gcgtttaccg tccggtgata ccgttgctgt caggtctcct
      421 gcggcgctgt actcataacg ggtttcacgc ccctgcgcat ccttctgact gaccagttgc
      481 ccacgcgggt tataactgct gtaagtgctg atgccttcct cccggtgaac ggcgatttgt
      541 tgaccgtacc ggtcgtactc ataccgcgtc gtgtaccccg agcagtccgt aaaggccagc
      601 agttgaccgt aacggctcca tgccatctgt tttgtactgc ccgtcgcgtc ctgtatccct
      661 gtcggcagct cactcgccgg ccgctttcag gcattgcggc tgtccggcca tcggcaccgc
      721 tgtccgatcc ggcaagtaat ggggggatca atactccgtt tatggatctg ccgcccattt
      781 atgccagccc cggcgatcgt aatgacgacg tctctgcggc agaaatggcg caacgccacc
      841 ttgcggtcac ccccttactg cgcggtcttg gcggctcgct taccatgagc aattccgacg
      901 atgcggatga ttttctggag gaggccggac gaacgttaca ggccgcaata aaaggtctgc
      961 tcgatttgca gcagcagcgt aacagcctct cagacaaaca tttgcgcccg ctggaagata
     1021 acccgctgcg cctgaacatg gattacgcca ccgcgctcga cgtaatgttt gccgaaggta
     1081 aaagcccggt acatctggcg gctcccgccg ccgtcagtga aagcctgcgc aatgtccgcc
     1141 accacgaaga agctaaccgg gcagcgattg tggagtcgct tcgtgtcctg ctggatgctt
     1201 tctcaccaca aaatctgctg cgccgctttg tgcagtaccg ccgcagccat gaactgcgcc
     1261 agccgctgga tgatgccgga gcatggcaaa tgtacagcca ttattacgaa gaactggcct
     1321 ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt ctacgcccaa gtctatgacc
     1381 gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt ttctggcctg
     1441 cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca gtgaggtgat
     1501 caaaaacccg gatatccagg tcgggagcct gaaggagcaa ccttcggaaa taaccgtcac
     1561 cctgctgacc gaaccggaca ccaataccaa tgcggaaggt gaatcagccg ccgtggatgt
     1621 gcaactggtg tacctgactg acgactcaaa attgctggca gccgactatg accagatagc
     1681 cagtacgccg ctaccggatg tgctgggtaa aaaatatatc gaccaccagg atttcaacct
     1741 gttgccggac accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa cgcagtttat
     1801 tggtgtcgtg gcctatttct ccgatgacca ggccactgaa tggaaacaaa tcgagacggt
     1861 ggaaggaacc ggccaccatt accgcctgct ggtgcatgta cgtcaaagca gcatcgaaat
     1921 gaaaaaagag gatgaataat tatggcaacc acccgcaaca aggtgatgtg gcaggaaggg
     1981 atgctgatgc gtccacacca tttccagcag cagcagcgtt acaacgacta cctggataac
     2041 cagcgtttcc gggccatgaa tgatttatcc tggggattta ccgaactcac tctcaacaat
     2101 gaactgctgg cacagggtaa gatcatgatt gacagcgcgt caggcacact gcccgacggc
     2161 accgtctttt ctatccccga ccaggacgca ctgcccgatc cgctgcaccc gcaacatttt
     2221 ccggacgaga gaagccgcaa tatctacctc gctctgccgg tcgccagtga tgtgagaaat
     2281 gaaatcagcg acgggcggcg aatcgggcgt taccggctga attatgccga tgtccgggat
     2341 ttgcattcag aagaaggtga cacgcgaaca ctgacgctgg gacaactgac gccgcgcata
     2401 tgagcggtgc agaagatatg agcgcctata ttacgctccc gctttgccgt atcagcgatc
     2461 gccatgccga tggctccctg acgttggatg acgattttat cccctcctgc cagaatattc
     2521 aggtcagtaa gaaactgcgt gtttatctca aagaggtaca gggggccatt ggcggacggg
     2581 caagcgatct ggcaaaccgt attggctctc cggcgcagag cggcatcgcg gatgtggcgg
     2641 aatttatgat gttgcagtta cttaaccgta accagacccg gtttacccat cgcgcccgcc
     2701 gatcccagct ccacccggaa gatttctacc ttgatcttgc tgggttgctg ggtgaactga
     2761 tgacctttac cgagccgtcg cgcctgccct gcccgcttga tgtgtatgat catcatgatc
     2821 tgaccaaaac atttaaaaca ctgttaccgg aagtcaaacg ggcgctgcat accgtactgt
     2881 cgccaagagc ggtcaatctg ccgctgcatc tgcgtgacgg tatctggcag gccgatatcc
     2941 atgacacaga actgctgcaa tctgccacct ttgtgctggc cgtggcggca aacgtgccgg
     3001 tcgatcagat ccagcgtcag tttatccagc agtcgaaaat ttcctcgccg gaaaaaatcc
     3061 gcaatatggt cagtgtgcag atccccggca ttccattgcg cgcactgatg gtggctcccc
     3121 gccagcttcc ttaccattcc gggttcagct atttcgaact cgacaagagc ggacaggcct
     3181 ggacagaaat ggctgccgcc ggggccgttg cactgcatgt atccggcagt ttcccggatc
     3241 tgaacatgca actgtgggcg ataagagggt aagacgatgg atgaggggtc actgtctctg
     3301 ccaccattta ccggatacga cgaaaaaagc cagcgcgact atcatctcgc gcttcgtggc
     3361 aacagcctga atccgatgat cgacgcagcc acgccgctcc tcggtatggt gatgcgcctt
     3421 tcaaccatga acagccagac gatgccggag catctttttg ctcaggtggt gacggatgtt
     3481 caggccgtgg agcaactgct tcaggagcag ggctacgaac cgggcgtgat tatctcgttt
     3541 cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg gtaacggctg gtcgaacaag
     3601 aacgagtgga tcaagcagtc attgctggtc catttccaca acgaagcctg gggtggagag
     3661 aaagttttca tcctgctgga gcgcctgatc cgtgaaccca aacgttacca ggatctgctg
     3721 gaatttctct ggctctgttt ttctctggga tttcgcgggc gctacaaagt ggcagcacag
     3781 gatcaggggg aatttgagca gatctaccgc cgcctgtacc acgtcctgca taaactacgc
     3841 ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa cccagggcgg gcgttatcag
     3901 ctcatcagtc gcctgaccat taaacatatt ttttgtggtg gcgttgtcgt gctggcgttg
     3961 ttttacctgt tctatctgtt gcggctggac agccagacgc aggacatcct gcaccagcta
     4021 aacaaattac ttcgataagg acgtttatga tccagattga tcttcccacg ctggtaaaac
     4081 ggcttaacct gttctcccgc caggcgctgg agatggccgc ctctgaatgt atgagccagc
     4141 aggcagcgga aattaccgtc agccatgtgc ttattcagat gctcgccatg ccacgcagtg
     4201 acctgcgggt tattacccgc cagggcgata ttggcatgga agagttgcgc caggcgctga
     4261 cggtggagaa ctacacaact gcccgttctg cggacagcta cccggcattt tccccgatgc
     4321 tggttgagtg gctgaaagag ggctggctgc tggcgtcggc tgagatgcag cacagcgaat
     4381 tgcgtggcgg cgtgttgctg ctggccctgc tgcattcgcc gctgcgttat ataccgcctg
     4441 ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct gcaacaggac tttgtgcaat
     4501 ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga cggtaaaggc gcaggcacac
     4561 tgacggacgc tgctgacacc ctgcttgccc gctacgccaa aaacatgacc gcagacgccc
     4621 gtaacggcag gcttgacccg gtactgtgcc gcgaccacga aatcgacctg atgatcgaca
     4681 ttctctgccg ccgccgcaag aacaacccgg tggtggtggg cgaagcaggc gtgggtaaaa
     4741 gcgccttaat cgaagggctg gcgctgcgca tcgtggcagg ccatgtgccg gacaagctga
     4801 aaaacaccga tatcatgacc cttgacttgg gcgcattgca ggccggagcg tcggtgaagg
     4861 gtgaattcga aaaacgtttc aaagggctga tggcagaggt catttcctcc ccggtgccgg
     4921 tcattctgtt tatcgacgaa gcacataccc tgattggcgc gggcaaccag cagggcgggc
     4981 tggatatctc caacctgctc aaaccggcgc tggcgcgcgg cgagctgaaa accatcgccg
     5041 ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga tgccgccctg tcgcgccgct
     5101 tccagttggt gaaggtcagc gaacccaacg ctgccgaagc caccattatt ctgcgcggtc
     5161 tgtcggcggt ctatgaacag tctcacggcg tgctgattga tgatgacgcc ttgcaggccg
     5221 ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt accggacaaa gcgattgatg
     5281 tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc gtcgccgccg aagcaaatct
     5341 cggcgctgac cactctgagc caccagcagg aggcggaaat tcgccagctt gagcgcgagc
     5401 ttcgcatcgg actgcgtacc gacacatcac ggatgaccga ggtgctggtg cagtatgatg
     5461 aaacgctgac ggcgctggat gaactggaag cggcctggca ccagcagcag acgctggtcc
     5521 gggagattat tgcgctgcgc cagcagttac tgggcgtggc agaggacgat gcggcgccgt
     5581 tgccggacgc agataccgtg gaggatacgc ag
//
LOCUS       DAZFJF010000219         5584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFJF010000219 DAZFJF010000000
VERSION     DAZFJF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.221"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(188..1378)
                     /locus_tag="ACHKAU_004818"
                     /pseudo
     CDS             complement(188..1378)
                     /locus_tag="ACHKAU_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131474.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1642..1842)
                     /gene="torI"
                     /locus_tag="ACHKAU_004819"
     CDS             complement(1642..1842)
                     /gene="torI"
                     /locus_tag="ACHKAU_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO5533724.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(1900..2067)
                     /locus_tag="ACHKAU_004820"
     CDS             complement(1900..2067)
                     /locus_tag="ACHKAU_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533725.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(2103..2402)
                     /locus_tag="ACHKAU_004821"
     CDS             complement(2103..2402)
                     /locus_tag="ACHKAU_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533726.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(2560..2838)
                     /locus_tag="ACHKAU_004822"
     CDS             complement(2560..2838)
                     /locus_tag="ACHKAU_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO5533727.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGYVIVVTTQSGMTIQIHKPKEEK"
     gene            complement(2838..3425)
                     /locus_tag="ACHKAU_004823"
     CDS             complement(2838..3425)
                     /locus_tag="ACHKAU_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5533728.1"
                     /translation="MTISTKKPLYAEVHNVPDDYEFTDEELNRIIAGDMFTPRQAAIM
                     AREIQKLRAAMLHGAEPVSQTYKSQHTQFEQVADLYEMQFDDGRTCAFHTDAQKAAQW
                     LQACDGNRVQEYVKLERLRNALSGNSPVTPDGWICCSERMPDKLIPVMVMYEDGEMWS
                     AMWNGNRWDDGTEYPDPHSVTHWREMPAAPQQEVK"
     gene            complement(3422..4039)
                     /locus_tag="ACHKAU_004824"
     CDS             complement(3422..4039)
                     /locus_tag="ACHKAU_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533729.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPIGEVSEKRLGLVMDGTVDLGGKSTYRIIKGEKAMKLLPLGTKFYTAPPAPIALEA
                     IENAIEYIRSIAFHIDEDDYHGKHIAYFMRQALAWLEGHSCSDDSQGKSDNSPLPRYQ
                     VIELTMLVKQLVSQLKKAKPDCKLPDRAMDYLLRNGLVSAEDVLR"
     gene            complement(4043..4231)
                     /locus_tag="ACHKAU_004825"
     CDS             complement(4043..4231)
                     /locus_tag="ACHKAU_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533730.1"
                     /translation="MSLTVRQLISKLRKMPPEAVVVWQGYDQSEGEYNDFVGHVADVT
                     DENAPSFDPEVRVVALRG"
     gene            complement(4233..4901)
                     /locus_tag="ACHKAU_004826"
     CDS             complement(4233..4901)
                     /locus_tag="ACHKAU_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5533731.1"
                     /translation="MSLIDYQALRAKAEKATCGVWSLEYGESRFDCDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKRRDQE
                     NEDIALTVGKLRVELEGKDKLIAELGKQCAEWERKALSNFEECAAMAERIEEMSKQSC
                     EARERDLFESWVMHSICISKSTLEGLRTETGYRNATLSGTDFNRMWKQWKSIRATGIR
                     IKGE"
     gene            complement(5378..5485)
                     /locus_tag="ACHKAU_004827"
                     /pseudo
     CDS             complement(5378..5485)
                     /locus_tag="ACHKAU_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5482..5584)
                     /locus_tag="ACHKAU_004828"
                     /pseudo
     CDS             complement(5482..5584)
                     /locus_tag="ACHKAU_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
BASE COUNT         1355 a         1373 c         1167 g         1689 t
ORIGIN      
        1 agtaatttct tagtatttca tcataagcgt ttatctatcg tctctttcta aattaaccgg
       61 tcgtattaca aggtgctgaa tctgattaag gaatgatact taggggatta tggctgactt
      121 aataattaga taactgataa atgactatca gggggatatc gttaagtcag cctttaaaat
      181 caagcgatta tatagctggt gactcttttc gaagagcatc aaggtagtca gcgtaccatt
      241 gcatcatttc tcgtcttcct tcaaggtaac gagcatggtt gtatgtacca cgaatgctgt
      301 ttttgtccgt atgtgcaagt tgcatttcaa tccatgcact attgaatcct tgctcgtgta
      361 gaacagtact cattgtatga cgaaatccat gcccggtagc ttttttgtca tagccaatct
      421 tcttcaaaac catattgacg cttgcatcgc tgataggcct gttaacatca tttcttccgg
      481 caaagatgta tctgtatctt ccagtcagtt ctttgaggat ttgcagagac tggattgcct
      541 gatctgacag agggacgata tgctccttcc tcattttcat ccgggtcgga ggaacttccc
      601 atatcttttg ggtaaaatcg atctcacacc attcccccat acgtaattct actgttctca
      661 gccctgtgag catcaacaat tttgtagctt cacgggttac aatactgccg gtttagtttt
      721 cgagtttgta taaaaactct ggtagttcag gaggaagcag gaaagggtaa tgtgttcgct
      781 tatagctttt cattgctata accagatcag aagctggatt gtatcgtgcc cgacctgtaa
      841 caatggcata ccgaaacact tcaccacatc gttggcgaac tttattagct agctcgcagg
      901 ctcctcggct ttcgatacgc tgaagaactg cgagaagttc aagaggttcg atttcactaa
      961 tgggtctgtt tccaatgtag ggaaacacat tatttttgaa tgctctgtcg acataatcag
     1021 cgtagctggc agaccagcct gaaacttttg cgttgtacca ctcggtggcg attgcggcaa
     1081 aagttcttcc ttcggaaagg tttttctcaa gattttcagc tttcttgact tcaccgggat
     1141 ctttaccatc ggcatgtatt ttcttggctt catcccgacg tttgcgagct tcagccaatg
     1201 taatagttgg ataggtgcca aatgatagtt tcttttcctt ccccgcatag tggtacttca
     1261 ttcgccagta ctttgagccg ttaggattga cctgaaggta gagcccacca ccatcggcaa
     1321 tcttataaat tttatctttg ggtttggctg tttcaacttg tcgagctgtc agtggcatct
     1381 gggggcctcg caatagattg accaagatgt gcccctaaat aggcccccaa atgtgacttg
     1441 atttacgttc ccctgaggag aattaaggcg actatttgag taacataagt aagggtatgt
     1501 gctgattata aaggcttttt gtggggtatg gtagatttca ggagaggtgt gcatggtgtc
     1561 ccctgcagac atctacttag agtggcaggg gattgattag aatggtattt tttagatgtg
     1621 agaaatattt tacccgctat tttacccatt agcgcggctt aagagcttat ttttgaattc
     1681 acaatggtca cgatataacc atcttgctcg accgtggata actttggctt taggcaggtc
     1741 gccggactta atccggtcgt agatgaaggt tttaccaaag ccagtatcag ccatgatgaa
     1801 tttcaaatca accagtgaat caggttgtag ttcgtgttgc atgagtgcta tctccgaata
     1861 gggaatcgaa cctgcaaatc aggcaataaa aaaccgccat caggcggctt ggtgttcttt
     1921 cagttcttca attcgaatat tggttacgtc tgcatgtgct atctgcgccc atatcatcca
     1981 gtggtcatag cagtcgttga tgtcctctgc ttcgataact ctgttgaatg gttctccatt
     2041 caattcacct gtgactcgga agtgcatttg tgatttcccc aaaagatgct tgagtgcgct
     2101 tcttattcga ttcgcacacc tggtatttcg ccttttgaaa tggctaagtc ataaatttgc
     2161 gcagcactat acccatctcg catccatgaa tctaaggcgc gaatagcctc gctacgcttt
     2221 ttatcttctc tttcattttt gatatcaacg aggacatcaa cgcaattaag gcatatgtgg
     2281 attttgtcct tacattcgat catggcagct ttaccatgat ttccgccaca cagtgagcat
     2341 aaatcttcag ggtctggctg gtatttctgt aacgttaaag ggttgaatgt tgaacaggcc
     2401 ataatcatct ccataaaaca aaactcgccg tagcgagttc agataaaaga aatccccgtc
     2461 agtgcgagga ttgttattgt cttttcttcg tgcatttgtc gcactttcgg caccatccag
     2521 ataggcacat ccgtccgcaa ttaacacata taggccacat catttttcct cttttggttt
     2581 atgaatctga atggtcatgc cgctttgagt ggtgactaca atgacataac caggctgaag
     2641 actgttaaga ttgaatgctt cgtaaaacga atccaaggcc agcgcttttt tattctttcg
     2701 gttccaccaa cgccatccct tgctacaggc tacactgaca atccactgcc cactcctgta
     2761 agccatataa aaccagatga gcaaaacctg aaggaatgct atccagtcaa taatcgtata
     2821 tttcgcgaag gagtccatca cttcacctcc tgctgcggtg ctgctggcat ttcacgccag
     2881 tgcgtaactg agtgcggatc cggatattcg gtgccatcat cccagcgatt gccattccac
     2941 attgcagacc acatctcacc gtcttcatac atgaccatta ccggaattaa cttatccggc
     3001 attcgctcac tacagcatat ccaaccatcc ggagttaccg gagagttgcc cgatagcgca
     3061 ttacgcaatc gctccagctt cacgtattcc tgaaccctgt ttccgtcgca tgcctgaagc
     3121 cattgcgcag ccttttgcgc atcagtgtga aaggcacaag tgcgaccgtc atcaaattgc
     3181 atttcgtaga ggtcagcaac ttgttcaaac tgcgtatgtt gtgacttgta agtttggctt
     3241 acaggttcgg caccatgaag catggcggcg cggagtttct gtatctcccg tgccattatt
     3301 gcggcctgac gaggagtgaa catatcacct gcaataattc tgtttagttc ctcgtcagtg
     3361 aactcataat catcaggaac attgtgaacc tcagcataaa gaggcttttt tgtagaaatg
     3421 gtcatcgtaa aacatcctcc gcacttacca gtccgtttcg caaaagataa tccatcgccc
     3481 tatcaggtaa tttgcaatca ggttttgctt ttttcagttg gctgaccaat tgtttaacca
     3541 gcattgttaa ttcgatcacc tggtagcgcg gcaatggtga attatcggat ttgccctgac
     3601 tgtcatcact gcatgaatgc ccttccagcc aggccaatgc ttgtcgcatg aaatacgcaa
     3661 tatgtttgcc gtggtaatcg tcttcatcga tgtgaaaagc gatactgcgg atgtattcta
     3721 ttgcattttc aatggcctcc aacgctatcg gcgctggcgg tgcggtataa aacttcgtcc
     3781 ccagcggcaa caacttcatc gctttttctc ccttaatgat gcgataagtt gatttcccgc
     3841 caaggtctac cgttccatcc ataacaaggc cgagtcgctt ctctgaaacc tcaccaatag
     3901 gctctgcttc cagcgatgcc agagcaattc gtgccagttc ttccgcttct tctgctggca
     3961 gtacaacgtt gctaccaggt ccgtatgttt cgcgccactg cttgattgtc agtagtcgct
     4021 ctttggtaat agtggtcatg ggttatcctc ttagcgccac aacgcgcact tctgggtcaa
     4081 aagatggtgc attctcatct gtaacatcag caacatgacc tacaaagtcg ttatactcac
     4141 cctctgattg gtcatagccc tgccagacca cgacagcctc aggaggcatt tttctgagct
     4201 tgctgattaa ctgcctaacc gtcagcgaca tctcactctc ctttgatgcg aatgccagta
     4261 gcgcggatag atttccattg tttccacatt cggttgaagt ctgtgcctga taaggttgcg
     4321 ttacggtatc cagtttcggt acgcaatcct tcaagcgtcg atttggagat acaaattgaa
     4381 tgcattaccc atgattcaaa caaatcacgc tcccgggctt cgcaactttg cttactcatc
     4441 tcttcgatac gttcagccat cgcagcacac tcttcaaagt tgcttaatgc ttttcgctcc
     4501 cattcggcgc attgttttcc aagctctgca atcagcttgt ctttgccttc cagctcaaca
     4561 cgcagtttcc ctaccgttag cgcaatatcc tcgttctcct gatcgcggcg tttgatgtat
     4621 tgctggtttc tttccagttc atccagtaat gccagcacag tagccggatt ggctgcggcg
     4681 atgaattcag cattggcctg ctgttccatt tggaaatctt catcgaaacc gctttctgga
     4741 tgtgctcctt caattctgca aatgggaaga tatccaacaa cttcacgatg aattaacgca
     4801 tcatcacaat caaatcggct ctctccatat tcgagcgacc atacaccaca cgttgctttt
     4861 tctgccttgg cacgcagtgc ctgatagtca atcaggctca ctggttgcct cctttgcgaa
     4921 tctgttccgc ccattcttca agggatttct ccgcatattc accagacagg ccatcaatcg
     4981 gatgcggttc attagccaac tcttctttcg ctgacagaat catgcgcgta acatcgaaaa
     5041 cttcacgtaa agacttattg ataaatccgt ggttgaaagc agcagcaaga cggcttgcgg
     5101 catagttaat accctcgttg cgtgcttccg cacgtacttc agccaggaaa gcatcggtgg
     5161 ctggggtttt gatttcgtta agcgcatcac tgaataaacc acgctccata cctagctctg
     5221 cttcgtaatc ggcatcgaat gcagcgtctt tgcagaactt cttcatcccc gcactctccg
     5281 ctgccagcgc cgcgcacttg gcctcaaggt tatcaatcgt gattccagca gaacgacact
     5341 cccgcaacgc cgtttccagt tttgattcaa gttcaccgaa cttacggaca agatattcag
     5401 cgtttgtttc gttaaccttt agatctcgtg ggatgcattt acctttcaga aaaccatcca
     5461 tctcaattag tgacatttgt ttcatttttt cccactccgc cacatagcat tcagatattt
     5521 gttgtcatta acagaaccga aactctttct cttaagcaat tactctctcg atggcattgg
     5581 cttt
//
LOCUS       DAZFJF010000220         5535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFJF010000220 DAZFJF010000000
VERSION     DAZFJF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.222"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..358)
                     /locus_tag="ACHKAU_004829"
     CDS             complement(<1..358)
                     /locus_tag="ACHKAU_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5533732.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLI"
     gene            complement(625..1290)
                     /locus_tag="ACHKAU_004830"
     CDS             complement(625..1290)
                     /locus_tag="ACHKAU_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO5533733.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1431..2072)
                     /locus_tag="ACHKAU_004831"
     CDS             complement(1431..2072)
                     /locus_tag="ACHKAU_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO5533734.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2781..3812
                     /gene="repA"
                     /locus_tag="ACHKAU_004832"
     CDS             2781..3812
                     /gene="repA"
                     /locus_tag="ACHKAU_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO5533735.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            complement(4772..>5535)
                     /locus_tag="ACHKAU_004833"
     CDS             complement(4772..>5535)
                     /locus_tag="ACHKAU_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533736.1"
                     /translation="VFHLNKSGDLLLTTSKTHGGGVIVIFKDFINNWWKYNLTLITVP
                     IDDKLSDNRINITPMGITIHEIVNGDDKLFFYPTPLKNGGFILHNPLYTNSFPSSYSH
                     ELFDLVEAYRNPMYSYLQNSIINRYIHTVSEHNGKDGVAQMSLPIYAYSTCRFWPKES
                     VAVGDTINLSNYEHIEFSFNSFSKDVCREQNSDIYKIFFKIVALNNSIVVSENMFTQK
                     NFLEHNTFFSVTGIDESLYEKHIIFITLKAIPPQK"
BASE COUNT         1538 a         1302 c         1384 g         1311 t
ORIGIN      
        1 caatcagtct gccggtgcgg tgtacgagag gacatgtcag cagcaggcaa agtggtgcaa
       61 ggagaatgtt cggggcagga gcctgcagca gatgaataat aagaccctgc aggagaatgc
      121 cggatatacc aaaaaaacct gccagcatac agaggacgat cagcaggggg atccgtccga
      181 tgttcagcca gtccagcgcc tctcctgtca gcccctccgg taaatcaggt gtatccatag
      241 ctgaagagag ggagtgtccg cagagcaggg cgataagttc agcgatacca gtaagcagaa
      301 caaaagtcag ggctgccaga tagggtacgt tatacgcggt aagtaacgtc agtgtcattt
      361 ttcctcctgt ggcaggtcag tcaaacaaca ttatggcgtg aagcttccgc ttctttaatt
      421 gtcagaagaa gcatatcgtc agtcatcaga agacaagggg atgtctgcag gaggggatga
      481 taacaactga agcctcacgc cggaatgcgc tttatgacaa ccggagagga cggataataa
      541 aaaatgggac atccacatca gcaaaccgcg atttgcgaaa aaaagtcagc ggagagtggc
      601 tgccgttgtg gctgtcccgg tatttcagtg taacaggtga ttgctgtgat ctatgcccca
      661 ttcggctttc ggtaaacctg aatgccccac atgaagaatg cgtgacagca tgtttctgcc
      721 gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata aaaccttcat cccggaatat
      781 ctcttccact tctgaagatt cgagggatgc cggatgaatt aatgaaaatg ccaccggtct
      841 gagcattctg cccgtggtgg aaaaggtgtc tctggtactg ttcagctgca ggatatgacg
      901 tttacctgtt tgcgcacggg aaagtgtctc actcagcagc tggtcgttcc atgcgcacgt
      961 gccaatctgc catcccagac ttctgcacgc ggaaagagcc gagcggaagc ggtacaggtc
     1021 gtataatgct gcgttattgc cactttccag cagtgatttc agcgtatttg ctaccggtat
     1081 gtccgtattg gtggccagca gttttcgcag agtcgtaaac tcttctgcca ttcctctgaa
     1141 tgcgctcatc tgacgcgcag attcaagcca gttcagcagg taacgccggc gacgccgttc
     1201 gttgttgata ctgtccgggg cggtggagag tgcttcgagc atattctcca gtaacagaat
     1261 gacagggttt tccagggata gaatggtcat aaaggcgtcc tgaaaaaatg cccgacagcc
     1321 gctctccgta caccgtaagg tgtcaccggt gccgggcatt gtggaaacag aaggaaaaaa
     1381 aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag acagcaattt tcatgtcaac
     1441 gctccgtcat cagataccag gtgcaggagc agggcgatcc tcgactccgg cagaggcggt
     1501 gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca gccagagggt ggaggcagca
     1561 tccagctcct ccctggctgc cggattgagc gcaggatccg ggtacacttt catcaaagac
     1621 tgaacgatat cctccctgac ggttttgatt tcaccggcca ttttcacgaa gttcagaaag
     1681 gcactgtgac ggcgaaggtg agtgaagctg tggcgttcgg ggtctgcttt caggaaaaag
     1741 taaccgggaa acacggcgtg tatatgtcga cggtaaccgc agactttatc cgtgcggcgg
     1801 acttttttca gtatcagggg tgtccaggga aggacgtgtt gttcggaaag ccaggaaaag
     1861 agatgttcgc ggtttttgcc ggcagggatg tactgagcca gataccagtt gcgttgatgt
     1921 aattctctga tattcaaaat ccggaacgct ccgcgaaaat cgatccccgc agaggggctt
     1981 cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg ctgatcctcg
     2041 ttcagcccgg cctgcagttc tgacaggcac atgatttacg cactcttgta agccttgtgg
     2101 acggtttcga ggccggcgaa aaccgacaaa acctgcaaac aggattaatg gctggcaaac
     2161 atattacata aggatatatc tgtggcaaga gcgaagataa gtagttaaat agatcgttat
     2221 atttgataaa gcagcatatt acgctgggtt gtgtgaggtg tttggatgat tagatcgtag
     2281 atcgtagatc gtaattacaa gatcgattca tccaaaaaac atgaaaaaca cagcataaaa
     2341 gatcgtttgt ggaattgtct attttttgac acagttcaaa aaaacgacgt tttttaggtg
     2401 gttaatgaga ggcctggtaa atcagagaaa tcgtgttggc ttttcaaagc ggtggaaaag
     2461 gggtatattg cggatcgtta tgcagtggct tttgggatcg tcaggatccg ggaagtcaga
     2521 aaatggcagg atgcgccata aggcattcag gacgtatggc agaaacgacg gcagtttgcc
     2581 ggtgccggaa ggctgaaaaa agtttcagaa ggccataaaa ggaaaacccc cataatcttc
     2641 tcatcgaact tggcggaacg ttagaagatt atgggggcct cacagaatac gggataagta
     2701 tatatgaaac cgtgtcagag attcaaccct tcgcagtgta ttaacgcacg gcattctgtt
     2761 tccgccataa gcgacagctt gtggcaggtc tgaagaatac tccatataac gcagtacact
     2821 ggagtcagtt agcacccgaa gagcagatcc gtttctggga agactatgaa gcgggaaggg
     2881 cgacctcttt cctggttgaa ccggaaagga agcgcaccag gcgccgtcgt ggcgaacact
     2941 ccactaaacc caaatgcgaa aatccgtcct ggtatcgtcc tgcgcgctat aaggagctga
     3001 gcgggcagct cgggcacgcc tacaaccgtc tggtgaaaaa ggccccggtg accggcgagc
     3061 agagcctgcg tatgcgtatg tcgcggcatc ctttttacgt gcagaaacgg acgttcgtcg
     3121 gtcgcaaata tgctttccgg ccggaaaaac aacgcctcct cgatgccatc tggccggttc
     3181 tggtcagctt cagtgatgcc ggcacacaca ctgtggggat gagtgtcact cgcctggcta
     3241 aagaaatcag tccgaaagac agtaagggaa atgttattcc ggaactggaa gtgacggtct
     3301 cccgtctttc ccgtctgctg gccgaacagg tgcgttttgg tgtgctgggt gtttcagagg
     3361 aaacgatgtg ggaccgtgaa acccgccagc gtctgccgcg ctatgtctgg ataacaccgg
     3421 ctggctggca gatgctgggc gtcgacatgg taaaacttca cgaacagcag cagaaacgac
     3481 tgcgtgaaag tgaaatccgc cagcagctca ttcgggaagg tgttctgcgt gaggatgaag
     3541 atatctccgt acatgcggcc agaaaacgct ggtatctgca gcgcagtcag gatgcgctga
     3601 aacaccgtcg tgcgaaagca gcagccagta agcgcgccag acgcctgaag aaactgcctg
     3661 ccgaccagca gattcatgag atggcagagt atctcaggaa gcgtctgccg ccggatgaag
     3721 cctatttttg ttccgatgac catctgaagc gactggccat cagggagttg cgtcagcttg
     3781 aactgacgct ggctgccccg ccaccgcact agacagcacc attccctcag cactgaatca
     3841 tcgccagccc ctccggggct ttcggcgcag gtatcgctca gcccaaaatc cgcagtaatc
     3901 accttaaatc ccctcagagg ggcatatttg ctcataaaac cacgcatcag tcatcagaac
     3961 gtggccacgt tgtttcagtt atccacataa atccgcaaac aaagaacttt aagaagctgc
     4021 aaacctgaaa cagcaaacct gcaatatagt cttaacccca ttatttaatc ccctgcgttg
     4081 cttcgccgca gcgaaagtct ttatctctga aaccactgtg aacaaacaca aagaggcctt
     4141 cgcttgcagc ggccaaggcc gcgccgctca gaatctaaaa gcacctccca cgctgacgcg
     4201 cgggccccga acctcaccgt tctggaacca caacaaaaaa acatcaggaa taaaaacacc
     4261 acacaaacgc agcaccgtgc ccacccctca taactgaaaa gcgccgccgc cccggcccaa
     4321 agggccggaa cagagtcgct tttaattatg aatgttgtaa ctaaacatca tcatcgcttt
     4381 aagaccccat ttgtctagca gggcgatggc ccactgcgtg aaccatcacc ctgcacacag
     4441 ggtattttat gtgcaattgt caaccactga atcggaaccc cagaggggcc cccacgattc
     4501 agaagttgac aatttaagca cagggactac atgagaaaaa tcgtggttcg tctgtcatac
     4561 acgcgcgtaa gcggcactga tagccctctt acgcggaaat acgcccataa gcgctaactt
     4621 aagggttgaa ccgacgccac ggtgtctcgt taagacttca attgcgtttt gtaggcagtc
     4681 agggatactg tcccatgaat ggccacctgt aagctccaga taaccatttt tgttattctc
     4741 cacaacgaat tccgcatacc ggaaatcagc gctacttctg gggaggaatg gccttaagag
     4801 tgataaagat tatatgcttt tcatagagag actcatctat gccggtaaca ctgaaaaatg
     4861 tattgtgttc caagaagttt ttttgagtga acatattttc gctcacaaca atcgaattat
     4921 tcaaagccac aattttaaaa aatattttat atatatcact attttgttca cgacagacat
     4981 ctttactaaa agaattaaag ctaaattcaa tatgttcata attagataaa tttattgtat
     5041 ctccaacagc tacactttct tttggccaaa aacgacatgt tgagtatgca tatataggta
     5101 atgacatctg agctacaccg tcttttccat tatgctcaga aacagtatgt atatatctat
     5161 tgataatact attctgtaaa tatgaataca ttggatttct atatgcctca accaaatcaa
     5221 ataattcatg tgaatatgaa gaaggaaatg aattagtgta aagtggatta tgtaagataa
     5281 aacctccatt ttttaatgga gtaggataaa aaaacaactt atcatcccca ttgactattt
     5341 catgtatcgt tatacccatt ggtgttatat ttatcctgtt atctgacaat ttgtcatcaa
     5401 taggaactgt tatcaatgta aggttatatt tccaccagtt atttataaaa tctttaaaaa
     5461 taactataac cccgccccca tgagtttttg aagtggttaa caataagtcg cctgatttat
     5521 ttagatgaaa aacct
//
LOCUS       DAZFJF010000221         5472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFJF010000221 DAZFJF010000000
VERSION     DAZFJF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.223"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..285)
                     /locus_tag="ACHKAU_004834"
     CDS             complement(<1..285)
                     /locus_tag="ACHKAU_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533737.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNHD"
     gene            809..1783
                     /gene="hemB"
                     /locus_tag="ACHKAU_004835"
     CDS             809..1783
                     /gene="hemB"
                     /locus_tag="ACHKAU_004835"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO5533738.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1889..2740)
                     /gene="tauD"
                     /locus_tag="ACHKAU_004836"
     CDS             complement(1889..2740)
                     /gene="tauD"
                     /locus_tag="ACHKAU_004836"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO5533739.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2737..3564)
                     /gene="tauC"
                     /locus_tag="ACHKAU_004837"
     CDS             complement(2737..3564)
                     /gene="tauC"
                     /locus_tag="ACHKAU_004837"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO5533740.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3561..4328)
                     /gene="tauB"
                     /locus_tag="ACHKAU_004838"
     CDS             complement(3561..4328)
                     /gene="tauB"
                     /locus_tag="ACHKAU_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO5533741.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4341..5303)
                     /gene="tauA"
                     /locus_tag="ACHKAU_004839"
     CDS             complement(4341..5303)
                     /gene="tauA"
                     /locus_tag="ACHKAU_004839"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5533742.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            5281..>5472
                     /locus_tag="ACHKAU_004840"
     CDS             5281..>5472
                     /locus_tag="ACHKAU_004840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533743.1"
                     /translation="MLRDEIAMIVCSPVLLCEQYSGIKTFIKRISRYRTDDFILSKKT
                     AKERLNMQLLRSNNWENYYT"
BASE COUNT         1331 a         1515 c         1355 g         1271 t
ORIGIN      
        1 gtcatgattc aggtaatatg tagagatagt cccgttaaca gtgtcaccat taaaggtggt
       61 atcgaaatca ccgtttgtgt tgttatagta gacaaagcca ctataattca catagttacc
      121 atctacagta cccggtgcat ccatattgct ccagtcaaga gcggtagttg gattgtcgta
      181 ataagtccag gtgtcataag tagttgcatt agcctgagag gtgatagcca gagtgcaagc
      241 caatgctaat tgtgatacta caagtttctt tttccaggag tgcatttgtc atccctcctc
      301 agggacgtaa taagttgatc catcaattat caatgcatag aaaacatatt atgtttcgcg
      361 ctaatgatta tgctgcgtta cagacgaatg gttcaattat tctttcccct aagtccgaat
      421 gtggacatat ataacctatg gagaaaaaca cgcatcaaat tgataaaagg gcgatatttc
      481 aacatgttgc agataatgtt atttttgata catacgcgtt ataaatgtca aaaacacaaa
      541 ccagataaat atcttatcaa tatacaacaa agtttgaatt attcaattta tagcagcaca
      601 accaaacgcc aaaaaataat aaccaagagc tataaataaa gattaaaaat attttcagtg
      661 aatatgaaat tattgctatt tctattacaa agatgtcctg gtctctcttt cattctcttc
      721 ttgtcaacat cacgacaact ttcgtaaaac actcctgccc cgcttcaggt atactgtgca
      781 ccttaattcc acaaacatca ggcagaccat gacagactta atccaacgcc ctcgtcgcct
      841 gcgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc ttaacgacct
      901 ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg aagccatgcc
      961 aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca tcgccaacgc
     1021 cggtatccgt tccgtaatga cttttggcat ctctcaccat accgatgaaa ccggcagcga
     1081 cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc agactgtgcc
     1141 agaaatgatc gtcatgtcag acacttgttt ctgtgaatac acttctcacg gtcactgcgg
     1201 tgtgctgtgc gagcatggcg tcgacaacga cgcaactctg gaaaatttag gcaagcaagc
     1261 cgtggttgca gctgctgcag gcgcagactt catcgcccct tccgccgcga tggatggcca
     1321 ggtacaggct attcgccagg cgctggacgc tgcaggcttt aaagatacgg cgattatgtc
     1381 gtattcgacc aagttcgcct cttcctttta tggtccgttc cgtgaagctg ccggaagcgc
     1441 attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgcg aggcgattcg
     1501 tgaatcactg ctggatgaag cccagggcgc agactgcctg atggttaaac ctgctggagc
     1561 atacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg gcgcgtatca
     1621 ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta tagatgaaga
     1681 gaaagtcgtg ctcgaaagct taggttcgat taagcgtgcg ggtgcggatc tgattttcag
     1741 ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taattttttt cctctcagaa
     1801 agggggagtt aacctgttga aagtacggtg aacgccccct caccctaacc ctctccccaa
     1861 gggggcgagg gaaccgtccg cactcgtatc accccgcccg ataaaacggt ttatccccaa
     1921 ggatcgtcgc ccgatgcata atccgccgct gtggcaggta atcggcattg gcatagtgct
     1981 gagtcacgcg gttatcccaa atcgcaatat cattcggttg ccagcgccaa cgcacctgaa
     2041 actccggttt agtgatatgg gcgaataaaa agcctaacaa ggcttcgctc tctttctcgc
     2101 tgacatcgac aattcgcgta gtaaagcctt cattcacaaa cagcgcctgt ttaccgctca
     2161 ccggatgcgt tcgcaccacc ggatgtagca acggaggatt tttcgcgacc gcctcgcgcc
     2221 agcgttgatg ttcctcctcg gttttgcggt atttgtattc cgggaacgat ttacggaaat
     2281 catgctccgc acgcagcccg ctcagcagct ggcggaaggg aacagagagc gcctcatagg
     2341 ccgcaatacc gctggtccag agcgtatcac cgccggtcga aggtaactct ttagctgcca
     2401 gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc ggtatgccag ttgtcgttat
     2461 ccggcggatt atcgttatgg gtatccagca cgatgatctc gtcaacccct tcggcatgag
     2521 ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc cagcgcgcgt tgctgctgcg
     2581 gcgcaatagt ttgatcgcgc agaaacacca cctgatggcg cagcaccgca tggtaaagct
     2641 gttcaaactg attatcgctt aacgggcgcg tcaggtcggc acccgaaatt tgtgcgccga
     2701 tatacggccc cagcggggta atgctcagac gttcactcat tgtacttctc catgccaggg
     2761 cgtcagacgg cgctgtaacg cgcgcaaacc cagttctaaa agaaaggcga taatcgcaat
     2821 caccgcgatc cccgccagca ccacgtcagt cgcgagaaat tcacccgctg actgaaccat
     2881 aaatcctaaa ccgcgcgtcg cggcaatcag ctccgccgct accagcgtag accagcccac
     2941 acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca aaatgacaaa
     3001 ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc gaacctgctg
     3061 cgcgcttttc acccccgcca gcgccgacat cgccaccggt gcaaaaatcg ctaaatagat
     3121 cagtaagatc ttcgaggttt caccaatacc aaaccagatc accatcagcg gcaaataagc
     3181 cagcggcggc accggacgat aaagttcgat taccggatcc agaatgccgc gcaccgtcgg
     3241 gcttagcccc atggcgatcc cgaccggaat accgatcacc accgccgcga gtagcgccag
     3301 cacaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca taaatccctg
     3361 cggtccggca atggtgagca gttttgccag tacttgttgc ggcggcggca aaaacagcgg
     3421 gctaatcagt tgcagcgccg ccaccgtcca ccatacagtg agtaaaaccg ctaacgtgcc
     3481 aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttcagccgcc gcgaatgcag
     3541 tttttcatta atgagcacac tcatgagaac gcctcccgtt gctcaaatac gcggcttaaa
     3601 acatattcgc gcatggcgat gaattgtgga tcggacttga tgctgcggct cgactctccc
     3661 gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca gcacgcggcc agggccgggt
     3721 gaaagcagaa ccagttcagt cgccataaac accgcttctt ctatatcgtg ggtaatcaac
     3781 agcacctgct tgcccgtctc ctgccagagt ttcagcagca gggtttgcat ctggtcgcgg
     3841 gtgaaggcgt ccagcgcacc aaacggctcg tcgagtaata acagctgggg attcgccgcc
     3901 agcgcgcggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca gatatagcgt
     3961 ttttctgcgc cttccagccc cacttttttc agcatctggt gcgcgatttc cagtcgctgc
     4021 attttctcta tacctgccaa ttgcaggccg aacgccacgt tgtcctgtac attgcgccac
     4081 ggtagcagcc cttcattctg aaaaaccacc ccgcgctctg ctcccggtcc ctcaatacgc
     4141 ttacccgcca gttgaatgct gccatgctga taaggcacaa aaccggcaat cagattcagc
     4201 agggtggttt taccgcagcc ggacggcccc agcaccacca gtagctcgcc gctttccagc
     4261 gtcaggttga tatcttccag tgccggtttg ccgccataat cggcgtaaag atgagagatt
     4321 tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg gctgtaatca
     4381 ttcgctacag ccgggacctt gccctgctct ttcaaaaact gcgcggtgtc gatgatcgct
     4441 ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata ggtattcccc
     4501 ttcaccagcc ccggaacgtc accttccggc acgccgctta aacgcgccag tttgctgatg
     4561 ttttccggct gtttcagcca cgcgtctggg ttagcaatgt acggttgctg agcatcgatg
     4621 gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcaaaatc tttgcgcacc
     4681 acccagacgt ccagcgttgg cgcaccccac tgcccgacct gttcagaatc ggtcagcacc
     4741 ttgccgtctt tttccagggc gttaaccgcc ggtgcccaga cataagcacc atcaatatct
     4801 ccccgctgcc aggcagcgat aatcgcgggc ggctgcaggt tcacaatctc cacttgcccg
     4861 ggtttaatgc cccagtgttt cagcgccgcc agcaggctgt agtgggtggt agagataaac
     4921 ggtacggcga tgcgtttgcc aatcagatct tccggtttgc tgatagtttt ctttaccacc
     4981 agcgcttcgg agttacccag ttttgacgcc agcaagaaaa cttcaatcgg cacctgttgg
     5041 ctggctgcaa ccgctaacgg gctggaaccg aggttgccga tttgcacatc gccagaagcc
     5101 agcgcccgca cgatgctggc tccgctgtca aacttacgcc agtccacggt tgctccgctt
     5161 tctttagcaa aggtgttgtc ggcctgagcc actttcgccg gttcggctga ggtttgatac
     5221 gcaacggtga cgttcaccgc ctgtgcctga aaagcgatga atgccagtgc ggcaagaagt
     5281 gtgttacgcg atgaaattgc catgattgtc tgctcccctg tcttgttatg tgagcagtat
     5341 tcaggaataa aaacattcat taaaagaatt agtcgttatc gcacagatga ttttattctt
     5401 agcaaaaaaa cggcaaagga aagattaaat atgcaattac tccgttctaa caattgggaa
     5461 aattactaca ct
//
LOCUS       DAZFJF010000222         5422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFJF010000222 DAZFJF010000000
VERSION     DAZFJF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.224"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..145)
                     /locus_tag="ACHKAU_004841"
     CDS             complement(<1..145)
                     /locus_tag="ACHKAU_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5533744.1"
                     /translation="MFSANSSATPALRGNEVNMDTLNLGNNESLVCGVFSNLDGTFTA
                     MTYT"
     gene            complement(258..665)
                     /locus_tag="ACHKAU_004842"
     CDS             complement(258..665)
                     /locus_tag="ACHKAU_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5533745.1"
                     /translation="MDTKNLTIGERIRYRRKNLKYTQRSLAKVLKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPSPPIEEPVALSPKEHELLELFNALPES
                     EQDAQLTEMRARVKNFNKLFEELLKARQRTNKR"
     gene            749..979
                     /gene="dicC"
                     /locus_tag="ACHKAU_004843"
     CDS             749..979
                     /gene="dicC"
                     /locus_tag="ACHKAU_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HFO5533746.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGELVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            963..1484
                     /locus_tag="ACHKAU_004844"
     CDS             963..1484
                     /locus_tag="ACHKAU_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5533747.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRITFNDGEVDQRALGVNRVKIFERWKAIDTRDKREKFTALIPAIMEAIRISDFRLY
                     REITDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKRAYRHQY
                     QQKAGWNYEPSFC"
     gene            1465..2430
                     /locus_tag="ACHKAU_004845"
     CDS             1465..2430
                     /locus_tag="ACHKAU_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533748.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTSSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKALGLLRSEKLNKSKRDMTNFYTINY
                     DSALLDDGKVNESIGSKCATPSGQNDTMEEVKMKRSIGSKRPNVIRSKWPDDPTENTT
                     ENTTENKKLSCPDASQPDELTAEQAFLTRHPDAVVFSVKKRQWGTREDLTCAQWIWGR
                     VVNLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFSRAQRDPFWI
                     KNIKSPEKLREKWDELVIRLWRGPAQRCVNHISEPDTEIPPGFRG"
     gene            2437..3177
                     /locus_tag="ACHKAU_004846"
     CDS             2437..3177
                     /locus_tag="ACHKAU_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5533749.1"
                     /translation="MKNITSGGVLARVSRFVPQDAIPPYRTVTEWREWQLAEGRKRSE
                     EVNRLNHQTRVEKIINRSGIQPLHRKCTFGNYRVQNDGQRHALSQAKSIAAELEGGCT
                     NFVFSGRPGTGKNHLAAAIGNHLLAKGRSVIVITVADVMLALHGSYDNKNSGEKFLQG
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASLLSVGMLTNLNHAAMNTLLGERV
                     MDRMSMNGGRWVTFNWESWRPNVSQHRN"
     gene            3207..3968
                     /locus_tag="ACHKAU_004847"
     CDS             3207..3968
                     /locus_tag="ACHKAU_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5533750.1"
                     /translation="METVLHALKTMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAGEGESGVTEEQPAQSEVPDVLTGEVEQKVTADMMIEFICQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCIPGADLPAEPEAASVAETDGKA
                     FPQPACVALPVREAETQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            3965..4144
                     /locus_tag="ACHKAU_004848"
                     /pseudo
     CDS             3965..4144
                     /locus_tag="ACHKAU_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4565..5116
                     /locus_tag="ACHKAU_004849"
     CDS             4565..5116
                     /locus_tag="ACHKAU_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5533751.1"
                     /translation="MTTQVTVKNLSPVTYNQIPVITTELLARLYGTEAIRIQQNHHEN
                     KSRFIEGKHFFKAVGDELKNLRLVLNESQNEVKISPKTRTLILWTERGAARHAKMLET
                     DQAWEVFEKLEDSYFNQYEKERAMGKMNIDFHLAIRDDKTAFIRSYAPGQLVTVEEAL
                     ALLKSRGWLVMPRDELAGKLMEL"
BASE COUNT         1455 a         1166 c         1521 g         1280 t
ORIGIN      
        1 tggtatacgt catcgcggta aacgtgccgt ccaggttgga gaatactccg cataccagag
       61 attcgttgtt gccaagattg agcgtatcca tgttgacctc atttcccctt aacgccgggg
      121 tagcggaact gtttgctgag aacaccgtgc ggtgtcttga tgcaagcaag attagccatg
      181 actaacaatt tggtcaagta tttttgtttg ccatgactaa cattttgggc aaccaaaaag
      241 ataacgcatt gattgcgtta tcttttgttt gttcgttgac gggcttttaa taactcttca
      301 aagagtttgt tgaaattttt tactcgggcg cgcatctcgg tgagctgagc atcctgttct
      361 gattcaggca atgcattaaa aagctcaagg agctcatgtt ctttggggga tagagcaact
      421 ggctcctcaa taggtggtga tggctgcttg tcttcatcgc caaatagaat ccatgttggc
      481 gagcactgca gtactttgct gagggcaaaa agattctttc ctgtaggttc gctatcatcc
      541 cgctcccatt gtgaaaccga tacgtgagaa attttcagga ctttagcaag agacctttgg
      601 gtgtatttga ggttttttcg gcgatatcta atgcgttcgc caatggttaa attttttgta
      661 tccatagtta gctaatgcta aatcttattg actatgtttt tgttaacatc tattttgtta
      721 gtcatggcta acaatcaagg tgctttaaat gcttaaaact gacgcacttt tgtatttcgg
      781 ttcaaaaaca aaacttgcac aagctgctgg tattcgtttg gcttcgcttt atagctggaa
      841 aggggagcta gtacctgaag gtcgcgcgat gcgcctgcaa gaggcatccg gcggggaact
      901 tcagtacgac cccaaagttt atgacgaata tcgtaaggca aagcgggcgg ggcggttgaa
      961 caatgaaaat cacccctgaa caggtttgtg aggctctgga tgcctgggtg tgccgaccag
     1021 gaatgacaca ggaacaggcg acgatattaa tcacggaagc attctgggct ctgaaagaac
     1081 gcccgaacat cgatgttcaa cgcatcacgt ttaatgatgg cgaggttgat caacgggcac
     1141 tgggcgttaa ccgggtgaag atattcgaac gctggaaggc tatcgacacc agggataagc
     1201 gtgaaaaatt cacggcgctg attccggcaa ttatggaggc tatccggatt agtgatttca
     1261 ggttgtaccg cgaaattact gacggaaaaa gcattacgta catgattgcc gggttaaaca
     1321 aagaatatgg cgatgtggtg gagtccgggc tgctttttgc agatccagct gttgtggaac
     1381 gtgagacaga cgagcttata gaaaaagcaa tcgctttcaa gcgtgcctat cgtcaccagt
     1441 accaacaaaa agccggatgg aattatgagc cttctttttg ctgaacgccc gctggttata
     1501 aacacacagc tggcgatgaa gattggttta aacgaagcca ttgtgttgca gcaactgcat
     1561 tactggttga gggataccag ttccggcatg gaatgtgatg gcgttcgctg gatttacaac
     1621 acaacggaac aatggctgga acagtttccg ttctggtcag agtcaacgtt aaagcgtgcg
     1681 tttgcaagtc tgaaagcgct ggggcttttg cgtagcgaaa agctcaataa atcgaagcgt
     1741 gatatgacta atttttacac aattaactac gatagcgcgc ttttagatga tggcaaagtg
     1801 aacgaatcca tcgggtcaaa atgcgccact ccatcaggtc aaaatgacac gatggaagag
     1861 gtcaaaatga aacgttccat tggttcaaaa cggcccaatg tcatcaggtc aaaatggccc
     1921 gatgatccta cagagaatac aacagagaat acaacagaga ataaaaaact ctcttgtccg
     1981 gacgcttcgc aaccggacga gttgacggct gaacaggcgt ttttaacccg ccatcctgat
     2041 gcagttgtgt tcagcgtgaa aaaacgccag tggggaaccc gggaggattt gacatgtgcg
     2101 cagtggatct gggggcgggt tgtaaacctg tacgaacagg ctgccagtga cgatggtgag
     2161 atcacgcgac cgaaagagcc caactggacg gcgtgggcca atgacgtgcg cacaatgcgg
     2221 atgctggatg gaagaagtca cagacaaatt tgcgaaatgt tcagccgggc acaacgggat
     2281 ccgttctgga taaaaaacat caagagcccg gaaaaactcc gtgagaaatg ggatgaactg
     2341 gttattcgac tgtggcgtgg tcccgcgcag cgttgcgtga atcatatttc tgaaccggat
     2401 accgaaattc cgccgggttt cagggggtag cgcatcatga aaaacattac gtcaggtggt
     2461 gttctggcaa gagtcagcag atttgtgccg caggatgcaa tccctccgta ccgtacggtg
     2521 acggagtggc gggaatggca gcttgctgaa gggcgtaagc gaagcgagga ggttaatcgt
     2581 ctgaatcatc agacgcgggt tgaaaaaatc attaaccgct ccggtatcca gccgcttcac
     2641 cggaagtgta cgttcggtaa ctaccgggtg cagaacgatg gtcagcgcca tgccctgagt
     2701 caggcgaaat ccattgcggc agagctggaa ggcggctgta cgaattttgt tttcagtggc
     2761 aggcctggca caggaaaaaa ccacctggcg gcggctattg gcaaccacct tctggcgaaa
     2821 ggtcgcagtg tgattgtgat aacagtggcg gatgtgatgc tggcgttaca tggcagctac
     2881 gacaacaaaa actcgggcga aaaatttttg cagggattgt gtggcgttga cctgctggta
     2941 ctggatgaaa ttggcatgca acgggatacg cgtaatgagc aggtcacgct gaatcagatt
     3001 gttgaccgca gaacggcatc gttactcagt gtggggatgc tgacaaatct taaccatgca
     3061 gcgatgaata cacttctcgg cgagcgggtg atggaccgca tgtccatgaa cggtggtcgc
     3121 tgggtgacgt ttaactggga gagctggcgt ccgaacgtca gccagcacag gaactgagaa
     3181 gtaattttta tccggaggaa gttttaatgg aaaccgtatt gcatgcactg aaaacgatgg
     3241 gtaaagccaa ttctgttgaa ctggcggcgc ggcttgatat cagccgtgaa gaagttctca
     3301 acgaactgtg ggaactcaaa aaaaatggcg ttgttgataa aacgggtcac acctggtttc
     3361 tggctggtga aggtgaatcc ggggtaaccg aagaacagcc agcacagtct gaagtaccgg
     3421 atgtgctgac cggggaggtc gaacaaaaag ttaccgcgga catgatgatt gagtttatct
     3481 gtcaggatgg ggctaaaacg tgtgaggaac tggcggataa gttcggtgtt agcattcgca
     3541 aggttgcttc cacgttggcg gtcgtaacag caacggggcg cctggcacgc gtaaatcaga
     3601 acggtaaatt tcgttactgc ataccgggcg ctgatttacc ggcagagccg gaagctgcat
     3661 ccgtagcgga aaccgatggt aaagcctttc ctcagccagc atgtgttgcg ttaccagtcc
     3721 gggaagcgga aacacaggaa gaaataaaaa cggaaagtgt ggcggtcaca gtgcagtcac
     3781 agccgtcgtt caccagaaag catccggatg ggctgatttt accatcgctg catgtggcta
     3841 accgcgagct gcgccgggca aaaggtcagg ttcagaagtg ggagcgagtc tgcgccgcgc
     3901 tgcgggagct gaacaagtgc cgggatattc tccgggatat taccgccacc agagaacagc
     3961 agcggtgagt gggtggaaga cgtggtgccg ggctgaaatt ctgatactcc ggcagtgcgc
     4021 gggaacgatg actgtcgaaa gtatcggcag tctgattggt cgtagcgagg cagcggtcag
     4081 agcgaaagcc cgggataagg gaataagcct gatgctgcag ggtgattttc gcctgtcagc
     4141 caaattaccg tataaagcga tggactgcct gaagcgaaac ggactgattg gcgctgagga
     4201 tagtttacga tgacctggcc tgaggcattc gtaacggcag gagttgcaat ggcggtagcg
     4261 ctggtggttt attcgatttg ccgctggggt tgacgtaatt actaatccgg ggctatattc
     4321 accgcacggt cgagttgacc gtcgggattg agaccccgga tattcaaccg cgacagacac
     4381 acgccgcgag cgtgtttttt attgtcgtat gcgcacgcac atctgaatta tggtgaggcg
     4441 tatggggcag ccgaaaggct ggccggttgg ttgatccggt agtctcaacc ctgtacgtct
     4501 cgccacctga tgattgagac ctgaaggtgg tgatagttaa attcatcaac cagagggcgt
     4561 tatcatgacg actcaagtta ctgtcaaaaa cctttcccct gtcacttata accagattcc
     4621 tgtaatcaca actgagttac tcgcccgcct ttacggaaca gaagcaatcc gtatacagca
     4681 gaatcaccat gaaaataaat ctcgatttat tgagggaaaa cacttcttta aagctgttgg
     4741 tgatgagctc aaaaatttgc gactagtttt aaacgagtcg caaaatgagg tgaaaatttc
     4801 tcccaaaacc cgcactctca tcctctggac agaacgcgga gcagcccgtc atgcaaaaat
     4861 gctcgaaacc gatcaggcgt gggaagtatt cgaaaaactc gaagacagtt acttcaacca
     4921 gtacgagaaa gagcgggcta tgggaaaaat gaatatcgat tttcacctgg cgatccgtga
     4981 cgataaaacc gccttcatcc gaagttatgc gcctggccag ctggtgacgg ttgaagaagc
     5041 cctggctttg cttaaatcgc gcggatggct ggtaatgcca cgagatgaac tggccggaaa
     5101 attaatggag ctttaaccga ttgagttaac cttgatatgg gaattcccat atcgggagaa
     5161 cattaccatc ggggataaaa tgatttgcgg ggaaagaaga gttaagtaga attgctgcgg
     5221 gtgcttgagg ctgtctgcct cgggcatgcc gccgtaaggc agacagagaa aagccccagt
     5281 taacattacg cgtcttgcag gacgcttaac attaatctga ggccaatttc atgctttgca
     5341 catgtaggtt agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa
     5401 aggcgatgtt aatcgccctg at
//
LOCUS       DAZFJF010000223         5319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFJF010000223 DAZFJF010000000
VERSION     DAZFJF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.225"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            180..752
                     /gene="lpfA-O113"
                     /locus_tag="ACHKAU_004850"
     CDS             180..752
                     /gene="lpfA-O113"
                     /locus_tag="ACHKAU_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO5533752.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            800..1531
                     /gene="lpfB-O113"
                     /locus_tag="ACHKAU_004851"
     CDS             800..1531
                     /gene="lpfB-O113"
                     /locus_tag="ACHKAU_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO5533753.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1556..4078
                     /gene="lpfC-O113"
                     /locus_tag="ACHKAU_004852"
     CDS             1556..4078
                     /gene="lpfC-O113"
                     /locus_tag="ACHKAU_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO5533754.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            4089..5162
                     /gene="lpfD-O113"
                     /locus_tag="ACHKAU_004853"
     CDS             4089..5162
                     /gene="lpfD-O113"
                     /locus_tag="ACHKAU_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO5533755.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPSPGQFTATATITVEIVR"
BASE COUNT         1504 a         1093 c         1228 g         1494 t
ORIGIN      
        1 ttgatttttt actgcttaaa taatatttaa acaataaatt tatattttat ttttacgtaa
       61 ttaatattat ttttagataa agccgtctct ttggtgtact attcctttga atcaaaaata
      121 cacttattaa tataagtgat gattcatggt aaaggatata ttatatcaat ggagttttta
      181 tgaagcgtaa tattataggc ggtgcattca ctctggcatc tctaatgctg gccgggcatg
      241 cactggcaga agatggtgtt gttcacttcg tcggtgaaat tgtcgacact acttgtgaag
      301 ttacctccga tacagccgat caaattgtcc cactgggtaa agtcagtaaa aatgcatttt
      361 caggtgtagg tagtctggcg tcgccacaga agttcagtat taagcttgaa aattgcccgg
      421 caacgtacac tcaagcagcc gttcgttttg atggtacaga agcgcctggc ggcgacggcg
      481 acctgaaagt gggtacgccg cttacagcag gcaaccctgg tgattttacc ggtacaggac
      541 aagcgattgc ggcaaccggc gttggtattc gtatttttaa ccagtccgat aattcgcagg
      601 ttaaacttta taacgactct gcttataccg ctatcgatgc tgaaggcaag gctgaaatga
      661 agtttattgc acgctatgtg gcaaccaatg cgaccgtaac ggctggtacg gcgaacgctg
      721 attcacaatt tactgtcgaa tataagaaat aatttatcaa gggccttttt attcagttgg
      781 ctttggccag ggagtcgtta tgtccagaaa aagttgttta atcaggctct ttaaaacaac
      841 agtttcatgt aagttattta ccgcgattat actcagcgtt tttttaggcc aaccagcatt
      901 aacttatgca ggcgttgtca ttggtggtac gcgggtggtt tacttaagta ataatgctga
      961 taaatctatt tctgtattta gcaaagaaga aaaaatacct tatctcatcc aggcttgggt
     1021 agatccattt aataaagagg ataaaagcaa agcgccgttt accgttattc cgccagtatc
     1081 acgattagaa ccttcgcaag aaaaaatatt acgcattatt cacaccaaag gtgtgtcatt
     1141 gcctgatgat cgcgagtcag tcttctggct gaatattaaa aatattccgc catcagcgag
     1201 caacaaagcg acgaactcgc tggaaatagc agtaaaaacg cgtatcaagc tgttctggcg
     1261 accagcaaat atccgcctta ttcccgaaga tgctgcgccg aaagtgaaat ggcgccggga
     1321 agggcgaaat ctgatagcag agaatcccaa cccaattcat atcagtgtga tggatgtcat
     1381 cgttgatggc catgatgtgc ccctcaatat gatccgtcct tttgaaacgc tcacgttacc
     1441 tttacctgct aattctgcgg gaggccaaat gacctggcga tttattaatg attacggcgc
     1501 agtcagtgac ccgataaaaa tgacgctgta agcaagggat aaggataggc gtattatgat
     1561 gacgaccaga atagtggttg gcctcacggc agggacgtgt ctgattttct cgcaaaacct
     1621 gatggccgag gtcagtgtat tcaatccggc gcttctggaa atcgaccatc aatccggagt
     1681 cgatattcgc cagtttaatc gggcaaacct gatgccccca ggtgtttata gcgttgatat
     1741 ttttatcaac ggtaaaatgt ttgaacgtca ggatgtgaca tttgttcagg ataatccaga
     1801 tgctgatctg cacgcttgct ttattgccat taaaaaaaca ctgtcctcct ttggcataaa
     1861 agttgatgcg ctcaaatcgt tcaatgatgt ggatgagacg gtttgcctcg atcctgctcc
     1921 acgtattgaa ggctcatcct ggcagtttga cagtgataaa ttgcagctga atatatccat
     1981 tccccaaatc tacatggacg cgatggctta tgattacatc agccccacgc gttgggatga
     2041 ggggattaac gcgctcacca tcaactacga tttttctggt tcacatacac tacgttcaga
     2101 ttatggttca caagagacag ataccagtta tctcaatctg cgcaatggac tgaatattgg
     2161 accgtggcgg ctacgtaatt atagtacttt aaacaccagc gatggccgtg cggaatacaa
     2221 ctccattagt acctggatac agcgcgatat tgccgcgtta agaagccaga ttatgattgg
     2281 tgatacgtgg acggcgagcg atattttcga cagtacgcaa attcgcggcg cgcgtttgta
     2341 tactgataac gatatgctac ccgccagcca gaatggcttt gctcctgtgg ttcgtgggat
     2401 tgcaaagtcc aacgccaccg tcatcattcg gcagaatggc tacgtgattt atcagtctgc
     2461 cgttccacaa ggtgcttttg agatcaccga tctcaacacc gccagtacag gtggcgattt
     2521 ggacgtaacc atcaaagaag aagacggtag cgaacaacga ttcacccaac cttatgcttc
     2581 attggcgatt cttaaacgtg aaggtcagac agatgttgat gtcagcgtgg gtgaattgcg
     2641 cgatgaagac ggatttacac cggacgtcct tcaggcgcaa atacttcatg gtttttccca
     2701 cgggatcact ttatatggag gtatgcaggc tgctgaaaat tatggttctg cagctctggg
     2761 tgtcggtaaa gatcttggcg ctttgggcgc aatttctttc gatgtgacac atgctcgtgc
     2821 gaattttagc catgatgata cagaaacggg tcagtcatat cgctttctct attcaaaacg
     2881 atttgacgac acagacacta gcttgcgcct ggttggctat cgttactcca ccgagggcta
     2941 ctataccctc aatgagtggg catcgcggcg caacagccct gaagactttt gggaaacagg
     3001 taaccgacgt agtcgcgtgg agggaacgct aacgcagtcg ttggggagag attatggcaa
     3061 tttatacctg acattaagcc ggcaacaata ctggcatacc gatgatgtcg aacgattaat
     3121 gcaatttggc tacagcagta gctggaagcg tctctcgtgg aacgtctcct ggagttattc
     3181 caatactgcc agacagggga cggggaacaa ccatgccagt gataacacca gtgagcagat
     3241 ctacatgctc tctttatctg ttcctttatc gggctggtgg ggtaatagtt acgccaccta
     3301 ttctgtttcg caaaacgata attccggtag ctcacatcaa ctcggactca gcggtacggc
     3361 gctggaacga aataaccttt catggaattt aatgcagtcc tataacagtc atgatgatga
     3421 ggttggcggt aatatgtccc tgacctatga tggctcttat ggcacggtga acggcagcta
     3481 taactacagc caaaattccc agaggctgaa ttatggtatc agagggggaa ttctggcaca
     3541 cagcgaaggg gtaacgttaa gtcaggagtt aggtgaaact attgctcttg ttaaagcacc
     3601 tggggccgcc gggttagaaa tagataatat gcgcggtgct gcgacggact ggcgcggcta
     3661 tacggtcaag acacagctaa acccttatga tgaaaatcgg gtagcaatca gcgataacta
     3721 tttctcgaag tcgaatatag aacttgataa taccgtcgtt acgatggttt ccacgcgtgg
     3781 tgcagtggtt aaagcggagt ttgtgactca tgtgggttat cgcgttctct tcagagtgtt
     3841 aaatgcaaat ggtaaaccgg taccttttgg agccattgct gcgatacaag atgcaagttt
     3901 ggcagattca ggaattgtcg gtgaccgtgg cgaactttat ctttctggtc taccagaaaa
     3961 aggacaggtt acgttatcct ggggagaaaa cgcctcaaca aaatgcatct tcaattattc
     4021 actttcgaca ccagaaagtg agagcggatt aattgaacag ggtgtgacat gtcattaaaa
     4081 ggatttttat gaacaagtat atcaaacagt ggtgctttgc tgtgtttatg ctctcgttaa
     4141 gtagtgtagc acttgcagct cctaaaggta tctgtacccc tgataatggc gtatttcaca
     4201 gtacgcttga tttctcggga tatcttatta ccgcgaacga aaataaggtt ggaacgacat
     4261 ttaacaccac tgtaactaat ggatcctcat atcctggacg ctgtcattgt gatactggta
     4321 acgtaggaga attcccctat atctattata cgtcaaaaat aaaccaggca ttaacttatg
     4381 ctggggttca ctctaatatt aattattatg atcttaatcc gaatcttgat gttgggattg
     4441 ctatagatat tcttggggtt gggtatgtga atgcaccttt tgaatatcat gctaacaacc
     4501 cgtctggtaa tacaaagtac aattgcaacc gcattgaacc tttaagtata tccagtgggg
     4561 ctaaagcgat agtatatttt tatatcaaga agacatttgc aggaaaattg attattcctg
     4621 aaacaaagat agtgacattg tatggaacaa ttagccgtga caccccggtg gattactcac
     4681 agccgatggc tgatgtttat attcggggcg atattacagc tccgcaaagt tgcgaaataa
     4741 ataatttaca gccagtttat tttgatttta aagaaatacc agctgcagat ttttcatctg
     4801 ttgttggaag tgcggtaaca acacataaaa ttactaaaac ggtcactatt gagtgtgaaa
     4861 acctggggat actgaatacc gatgatatca gtacgtcttt ttatgctacc gaacccaata
     4921 cggacaattc aatggtcgta acttcaaact ctaacgtggg gataaaaatt tacgacaaaa
     4981 ataataagga aatcaaagtg aacggtggtg agttgccaac agacatgggt aaatcaacag
     5041 tgtatggtga aaaatcaggt agtgtaactt tttcagctgc tcctgcgagc cttactggag
     5101 ctcgcccatc cccggggcaa tttactgcaa cagcgacgat aactgttgag attgtgcgct
     5161 aataggtctg acaacgattg ttcatcacat cgttgtcatc tcacccattt ctttatcact
     5221 tttaaatgca tcatgacaaa aagatgacat gtgggatttt catcttcaca tttaaaatca
     5281 gtaagttatt tggtaattcc tttctgtcat aaaaatgtc
//
LOCUS       DAZFJF010000224         5233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFJF010000224 DAZFJF010000000
VERSION     DAZFJF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.226"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(145..423)
                     /locus_tag="ACHKAU_004854"
     CDS             complement(145..423)
                     /locus_tag="ACHKAU_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5533756.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            complement(425..616)
                     /locus_tag="ACHKAU_004855"
     CDS             complement(425..616)
                     /locus_tag="ACHKAU_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO5533757.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            complement(637..1008)
                     /locus_tag="ACHKAU_004856"
     CDS             complement(637..1008)
                     /locus_tag="ACHKAU_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533758.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            1106..1408
                     /locus_tag="ACHKAU_004857"
     CDS             1106..1408
                     /locus_tag="ACHKAU_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5533759.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            1405..1830
                     /locus_tag="ACHKAU_004858"
     CDS             1405..1830
                     /locus_tag="ACHKAU_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5533760.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            1853..2815
                     /locus_tag="ACHKAU_004859"
     CDS             1853..2815
                     /locus_tag="ACHKAU_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5533761.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            2822..3563
                     /locus_tag="ACHKAU_004860"
                     /pseudo
     CDS             2822..3563
                     /locus_tag="ACHKAU_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3589..4350
                     /locus_tag="ACHKAU_004861"
     CDS             3589..4350
                     /locus_tag="ACHKAU_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5533762.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            4383..4664
                     /locus_tag="ACHKAU_004862"
     CDS             4383..4664
                     /locus_tag="ACHKAU_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5533763.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            4661..4888
                     /locus_tag="ACHKAU_004863"
     CDS             4661..4888
                     /locus_tag="ACHKAU_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533764.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            4881..5192
                     /locus_tag="ACHKAU_004864"
     CDS             4881..5192
                     /locus_tag="ACHKAU_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533765.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT         1355 a         1119 c         1498 g         1261 t
ORIGIN      
        1 ccctgcgttc tgtggggcgg ggtggtattt ttagtttaat aaacattaaa cttaagtcaa
       61 gtaaaagcta aaccgcggga cataacaaac acaacgcttt tgataaagtc gttgcggttg
      121 ttatgtttct attggtagtg aaagttaggg aaactggcgt cttgcgtgga tcacgtttac
      181 tacttcaacg cttgatgttg ttacgcggta tagaattata tagttagggt gggctacaat
      241 ctcacgcaag ctaggtactc tgtcgcttgg tgggtataaa tacggatgtt cggataacgg
      301 cagcacacaa ccccttaatc gctgccataa gcgttcagcc gcatctatgt cgaaacgagc
      361 aatataacta gttatatcat ctaggtcggt atctgcgctt tcaagccata acacgggtaa
      421 cattttactg cttgctccgt tccttgcgca tcttagcaaa gcgttctgcc attctgcgct
      481 caacttcgtc atggggaatt gctgggcgcg gatctgcaag gctcgttgct actttcgcac
      541 gcagccattc gttgtaactg ttttcttgtt caatggtttc aaattcagaa accattggtg
      601 aaagggctct attcatgttt cctccggttt tataactcag gcgcggcggc atttttgcgc
      661 cgcaatccat ctcgctatga gatcttccat tgattctttt ttctgcttta actcgctgat
      721 tatctggcgt tgctcatcct cagggaaggc tgaaaaaatc tgcaataatt ccagttgatt
      781 agacgttaac cctgcatgtg gtggagaaac ccccggttgt tctgcgtatt ccgcatccag
      841 atacccttcc ggcatcccgt atgtttgctc tattcttctg gcagcctttt ctccaaacga
      901 ggctctccca ctcattagtt gagataggta gctcttctct ttgggtggca gagttttatc
      961 tttaaaccac tccttgagac gtaaacggcg aatttctttt ttttgcatgt ggtaattatc
     1021 tttagtaatc actaaacaag caaatacttg acttaatggt ttattaaaca ctaaactcgc
     1081 aaaaaaacac taaaccgagg aaggtatgac attaaaagag tttattaaat cattaagggt
     1141 tggtgatgct aagaaattcg cggccagact tggtgtatcg ccatcttact tatcgcaaat
     1201 ggcgtctgga cgaacagcta tatctccaac ccgcgccctt atgatcgaat ctgcgacgga
     1261 aggccaagta agtagggcgg agctacgacc tcatgattgg gagcttattt ggcctgagta
     1321 tgcgagcggc attcgtttgg ggcaaacaca tgtagttcat gctgaaggtg attgtagtgc
     1381 atgcttatct gatggagttg attcatgaaa atcaagcatg aacacatccg catggcgatg
     1441 aatgtctggg cgcatccgga cggcgaaaaa gtaccggctg cgaaaattac caaagcgtat
     1501 ttcgagctgg gaatgacgtt cccggaactg tatgacgaca gccatccgga agccctggcc
     1561 cgtaataccc agaaaatttt ccgttggctg gataaagaca cccctgatgc tgttgaaaaa
     1621 atgcaggctc tgttaccggc gatcgaaaag gcgatgccgc ctttgctggt ggcccgtatg
     1681 cgcagccaca gttctgaata ttaccgtgag atcgtcgaac ggagggatcg gctggtgaag
     1741 gatgtcgatg attttgttgc gtcagcggtt gttttgtatg accagatgaa tcgcggcggc
     1801 ccggcaggga atgctgtggt gatgcactaa aagcacggtg ttcgggggtt ttatgagcag
     1861 caagcttcat ggtcttgtct gggaagggtg cgccttcacc ggcatgatct tatccagggt
     1921 ggcggttatg gcccgtcttg cagactacag caatgacgag ggcgtgtcat ggcctgccat
     1981 tgaaactatc cggcgtcaga tcggtgcaag aagtgaatcc acagtgaaat cggctattgc
     2041 agaactggcg aaagagggct ggctgacgaa ggaagagcgt aaggtcggtg ggcgtaatgt
     2101 aagcaatatc tatcggctta atgtggaaaa actcgaagca gctgcggcgg cggcgcgtga
     2161 gtcatataaa ccgaaaagaa aaattagccc ggcaaaaaat gacccgttaa cagttgaccc
     2221 gtcaaatatt gccccctcaa cggttgaccc gtcaaatttt gatggatcaa ctgttgataa
     2281 caaactgccg attagggggg cgatgattga ccccgatccg tcagtattaa aacctgatcc
     2341 gtcagataaa agatcttctt gtccggacgc ttcgcaaccg gacccgcaga cggctgaaca
     2401 ggatttttta acccgacacc ctgacgcggt tgtgttcagt gcgaaaaaac gccagtgggg
     2461 aagtcaggaa gatttggtgt gcgcacagtg gatctgggga cgaatcgtga gtctttacga
     2521 gcaggcggcc agcgatgatg gcgagatcac gagaccgaaa gaacccaact ggactgcatg
     2581 ggccaatgac gtgcggacaa tgcggatgct ggatggcaga actcacagac aaatttgtga
     2641 aatgtttggg cgtctccagc gggattcgtt ctgggtaaaa aacatcatga gtccggcaaa
     2701 actccgggaa aaatgggatg aactggttat ccgcctgggg cgttcgcccg cgcagcgttg
     2761 cgtgaatcac atttctgaac cggacactga aataccgccg ggattcaggg ggtgacgtgt
     2821 catgaaaaac attgcggcag ttggggttct tgaacgtatt cgcagacttg caccacaggg
     2881 gggcggttcc accgtaccgg acggtggagg agtggcggga atggcaactt gctgaaggac
     2941 gaaaacgcag cgaggagatt aaccgcctga atcatcaggt gcgggttgaa aaaatcctga
     3001 accgtgcggg catccagccg cttcacagga agtgctcatt cgggaactac cgggtgcaga
     3061 acgacggtca gcgccatgct ctgagccagg cgaaatccat tgccgatgaa ttgatgaccg
     3121 gatgtacaaa cttcgtgttc agcggtaaac ctggtaccgg taaaaatcac ctggcagcag
     3181 cgattggcaa tcggctgatg gcgaagggga gaagcgtgat tatcgtcacc gtgtccgatg
     3241 tcatgagcgt gttgcatgac ggctacgaca acggccagtc cggggaaaaa tttttacagg
     3301 agctttgtgg agttgacctt ctggtccttg atgaaattgg catgcagcgg gatacgcgca
     3361 acgagcaggt cacgctgaac cagatagtcg accgcagaac ggcttcgatg cgtagtgtcg
     3421 gaatgctgac gaacctgaat cacgcagcga tgagcacact cctcggagat cgggtgatgg
     3481 accgtatgac catgaatggt ggtcgttggg tgaattttaa ctgggagagc tggcggtcaa
     3541 acgttggacg tcagggtatg tgagaatttt tgacgaggta aattttcgat ggaaactgta
     3601 ttgcatgcac tgaaagcgat gggaaaagcc aattctgttg aactggcggc gcggcttgat
     3661 atcagccgtg aagaagttct taacgaactg tgggaactca aaaaaaatgg cgttgttgat
     3721 aaaacgggtc acacctggtt tctggctgtc gaaggtgaag ccggggtaac cgaagggcag
     3781 gcactacaac ctgaagcgcc ggatgtggta accgaagagg tcgctccaaa agttaccgca
     3841 gacatgatgg ttgagtttat cggtcaggat ggtgctaaaa cgtgtgagga actggcgggt
     3901 aagttcggcg tcagtactcg caaggttgct tccacgctgg cggtggtaac cgcaacgggg
     3961 cggctggcac gcgttaatca gaacggtaaa tttcgttact gcatgtcggg gggtaattta
     4021 ccagcagatc cgaaagccgc gccggtaacg aaaaatgatg gtaaggcctt tcctcagcca
     4081 gcaggtgctg cgttaccagt ccgggaagcc gcaacacagg aagaaataaa aacggaaagt
     4141 gtggcggtca cagtgcagtc acagccgtcg ttcaccagaa agcatccgga tggtctgatt
     4201 ttaccatcgc tgcatgtggc taaccgcgag ctgcgccggg caaaaggtca ggttcagaag
     4261 tgggagcgag tctgcgccgc gctgcgggag ctgaacaagt gccgggatat tctccgggat
     4321 attaccgcca ccagagaaca gcagcggtga gtgggtggaa gacgtggtgc cgggcggaaa
     4381 tcatgatact ccggcagtgt gcgggaacga tgaaggtaaa aagcgttggc gcacttatcg
     4441 gacgaactga agcggcagtg agaacgaagg cacgggagct gggcatcagc atgatgttac
     4501 gtggtgattt tcacccgtcg gcaaaatatt ctcagcgtga tattgagctg gcgcggcaac
     4561 tgcatcagag aggcatgcaa agaagggaaa ttgccagaaa attaggcatg ccgctgcgca
     4621 tagtgaataa ctacgtttat ttcgacagga gggtgtctgc gtgaaaatcc tgtatcagga
     4681 ttacggcccg gtggggcagg tggttatcag cagtactgta atggagtttc ggaagcataa
     4741 ccgtgtggtg gatgctgtgc tgttaacctg tccggggata tcggcgagtc gtgcaggtgt
     4801 gtttattatg aagacgaaat tatatggcag taaggcgtgg ataaagaagg cgtatcgtgt
     4861 agcgttgcag gaggttaaca gtgagtgaaa ttaaagaaat gccggtagtt cgtgacggat
     4921 atggctactg gacacatcct gaatatgaaa aattctgtga tggtcgggaa tatatttcaa
     4981 cggaagagtt taacgcctgg atggaggaaa ataatcttca atacgtcctc tgcttcagag
     5041 atgaaggatg tgctgacctt gatgcgtgtg atgctgatat ttctgcatgg gaaccggaac
     5101 gaccagaggg cgatggctgg tttattggtt ccattcatga tacggaagat ggcccggttt
     5161 gtgtctggtt gcgaaataag gctgaagcat aaaggcgata aaccaactaa caactaaata
     5221 ctgaagattt aaa
//
LOCUS       DAZFJF010000225         4993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFJF010000225 DAZFJF010000000
VERSION     DAZFJF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.227"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..170
                     /locus_tag="ACHKAU_004865"
     CDS             <1..170
                     /locus_tag="ACHKAU_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO5533766.1"
                     /translation="HGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            613..4515
                     /gene="espP"
                     /locus_tag="ACHKAU_004866"
     CDS             613..4515
                     /gene="espP"
                     /locus_tag="ACHKAU_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO5533767.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4763..>4993)
                     /locus_tag="ACHKAU_004867"
     CDS             complement(4763..>4993)
                     /locus_tag="ACHKAU_004867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533768.1"
                     /translation="TAQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCFMKTNAPRLF
                     FVDKESGVSFDVFHVLSVSFPVTACATHDGGM"
BASE COUNT         1516 a         1133 c         1113 g         1231 t
ORIGIN      
        1 accatggtgg tgagcgggga gcgctactgt acagcctgat cgggacgtgc aaactgaatg
       61 acgtggatcc agaaagctac cttcgccatg tgcctggcgt catagcagac tggccggtca
      121 accgggtcag cgaactgctt ccgtggcgca tagcactgcc agctgaataa cacatccccg
      181 tcaatacggc cctcgctgta cgcttacgca taaatattga atacatttac tgagcatgca
      241 aaagaaaaag acaaactaat cccggccttt cacagaattt cctctatcag aggctgagtt
      301 gacaacctgc tcacccagga aatagtaaca cccgaaaaca tgaagagact ctgataaaca
      361 atctcgatac atattcctca tctttcacag catctcactt ctttaacagg aattattatt
      421 atctacgcct cctcctttca tgtatccctc atgaatgatt aagataaaca tactgtgttt
      481 gttatccgta tgataacaaa cacataaagg agtaaacatt tatctggatg atgattacaa
      541 tgttcacgac tgtcagagac acacctctga tacattagaa aaaccaatgt ttcccttaaa
      601 aatggagctt atatgaataa aatatactct cttaaataca gccatattac aggagggtta
      661 atcgctgttt ctgaattatc cggcagagta tcatcaagag caactggtaa gaaaaaacac
      721 aaacgcatac ttgcattatg ttttttaggc ttattacaat cctcatattc ttttgcgtca
      781 cagatggata tttcaaattt ctacatccgt gactatatgg attttgcaca gaacaagggc
      841 atatttcagg ctggcgcaac aaatattgaa atagtgaaga aagatggctc caccctgaaa
      901 ctaccggaag taccatttcc tgacttctca ccggttgcaa acaaagggtc aaccacatct
      961 attggtggtg catacagtat cacagccaca cacaatacga aaaaccacca ctcagttgcg
     1021 acgcaaaact gggggaacag cacgtacaaa caaactgact ggaatacttc acatcctgat
     1081 tttgcagtat cccgacttga caagtttgtt gttgagaccc gaggtgcgac tgaaggcgca
     1141 gatatttcgt tatcaaaaca gcaggcactt gaacgttacg gggttaatta taaaggagaa
     1201 aagaaactta tcgcattcag agccggctct ggtgtggtat ccgttaaaaa aaatggacgc
     1261 ataactccat ttaatgaggt ttcttataag ccagaaatgt taaatggctc tttcgttcac
     1321 attgatgact ggagtggatg gctgatatta accaacaacc agtttgatga gtttaataac
     1381 attgcctctc agggtgacag cggttcagca ctgttcgtct atgataacca aaagaaaaag
     1441 tgggttgtcg ctggaactgt ctgggggatt tataattacg ccaatggcaa aaaccacgca
     1501 gcatacagta aatggaacca gacaaccatt gacaacctga agaacaagta ttcttacaac
     1561 gtggatatgt caggagctca ggttgcaacc attgaaaatg gaaaactgac aggcactggc
     1621 tcagacacca ccgatataaa aaataaggac ttaatattta ctggcggtgg agatatcctc
     1681 ctgaaatcct cttttgataa tggtgctggc ggtcttgtct ttaatgataa aaagacctat
     1741 cgagtaaacg gggatgattt cacctttaaa ggtgccggtg ttgatacaag aaacggcagc
     1801 accgttgagt ggaatatccg gtatgataat aaagacaacc ttcacaaaat tggtgatggc
     1861 acattagatg tccgaaaaac ccagaacacc aacctgaaaa caggtgaggg tcttgtcatt
     1921 cttggagctg aaaaaacatt caataatatc tacataacca gtggtgatgg aactgtccga
     1981 ctgaatgcag aaaatgcact gtctggcggt gaatacaacg gtattttctt tgcgaaaaat
     2041 ggcggaactc ttgacctgaa cggatataat cagtctttca ataaaattgc tgcaactgat
     2101 tcaggtgctg taataaccaa tacgtcaacc aaaaaatcca ttttatccct gaataatact
     2161 gctgactata tctatcacgg caacataaac gggaatctgg acgtacttca gcatcatgag
     2221 acgaaaaaag agaaccgtcg tcttattctt gatgggggcg tggacacaac aaatgatata
     2281 agcctgcgta atacacaact gtccatgcag ggacatgcca ctgaacatgc catttatcgg
     2341 gatggagctt tctcttgttc actaccagct cctatgcgct ttttgtgtgg cagtgattat
     2401 gttgcaggaa tgcaaaatac agaagctgat gctgtaaaac aaaacggaaa tgcctataaa
     2461 accaacaatg ctgtctctga tttatcgcag ccagactggg aaaccggaac attcagattt
     2521 ggaacgctac atcttgaaaa ttccgatttt tctgttggtc gtaatgcaaa tgtaatcggg
     2581 gacattcagg ccagtaaatc aaacattact attggtgaca ctacagcata tattgatttg
     2641 catgctggta aaaatattac cggtgatggt tttggcttcc gccagaatat tgtgcgtgga
     2701 aactcacaag gagaaacgct gtttacagga gggatcacag cagaagacag cactatcgtt
     2761 attaaagata aagcaaaagc attattttca aattatgtat acctgctgaa cacaaaagca
     2821 accatagaga acggtgctga tgtgacaact caaagtggta tgttctccac gagcgatatc
     2881 agcatctctg gtaatctgtc catgacaggc aatcccgaca aagacaataa attcgagccc
     2941 tcaatatatc tgaatgatgc ttcttatcta ctgactgacg actccgccag actcgttgcc
     3001 aaaaataaag catctgtggt gggagatata cactccacta aaagtgcatc catcatgttt
     3061 ggtcatgatg aaagcgacct ctcgcagttg tctgacagaa cctcaaaagg gcttgcactt
     3121 ggtcttttag gtggctttga tgtctcatat cgcggttcag tcaatgcccc gtcagcatct
     3181 gccactatga acaatacctg gtggcaacta accggagatt ctgcgctgaa aacactgaaa
     3241 agtacaaaca gcatggtcta tttcactgac agcgcaaaca ataagaaatt ccatacgctg
     3301 acggtcgatg agctggcaac cagcaacagc gcctatgcga tgcgtacaaa cctttctgaa
     3361 tcagacaaac tggaggtcaa aaaacacctg tctggtgaga acaatatttt actcgttgat
     3421 ttccttcaga aaccaacgcc tgaaaaacaa ctgaatattg aactggtaag cgcgccaaaa
     3481 gacaccaatg aaaatgtctt taaagccagt aaacaaacca ttggtttcag tgatgtaacg
     3541 ccggtcatta caaccaggga aaccgatgac aaaataacat ggtcactgac aggctataac
     3601 acggtagcaa acaaggaagc aacccggaat gccgccgccc tgttctctgt tgactataaa
     3661 gcgtttctga acgaggtcaa caacctgaac aaacgtatgg gtgacctgcg tgatatcaac
     3721 ggcgaagccg gtgcatgggc acgcatcatg agcggtaccg gctctgccag tggtggtttc
     3781 agtgacaact acacgcacgt tcaggtcggg gtcgacaaaa aacacgagct ggacggactg
     3841 gatttgttta ccggtttcac tgtcacacac actgacagca gtgcctccgc cgatgttttc
     3901 agtggtaaaa cgaagtctgt gggggctggc ctgtatgctt ccgccatgtt tgattccggt
     3961 gcctatatcg acctgattgg caagtatgtt caccatgata atgagtacac tgcaaccttt
     4021 gccggactcg gaacccgtga ttacagcacg cattcatggt atgccggtgc agaagcgggc
     4081 taccgctatc atgtcactga ggatgcctgg attgagccac aggctgagct ggtttacggt
     4141 tctgtatccg gtaaacagtt tgcatggaag gaccagggaa tgcatctgtc catgaaggac
     4201 aaggactaca atccgctgat tggccgaacg ggtgtggatg tgggtaaatc cttctctggt
     4261 aaggactgga aagtgacagc ccgtgccggt ctgggctacc agttcgacct gctggctaac
     4321 ggcgaaaccg tattgcggga tgcatctggt gaaaaacgca tcaaaggtga aaaggacagc
     4381 cgtatgctga tgtccgttgg cctgaatgca gaaatcaggg ataacgtccg ctttggactg
     4441 gagtttgaga aatccgcctt tggtaagtac aacgttgata atgcagtcaa cgctaatttc
     4501 cgttactcgt tctgatactc cgtattcaaa agggcctgcg ggccctttac gctcagtgcc
     4561 tgccgccctg atatgtctga gagacatttc cgggatgtca gtcgactggt ttacttaact
     4621 gcgatgacta aagctggcag taatgccggc ttttttacgg ccacatacgt cttcctctca
     4681 tccggaactg tgatacaaat cctgcataca ggattgaccg ccggttcctg caaatggcac
     4741 attttcagta agatagcgaa ggttacattc ccccgtcatg tgtggcacaa gctgtcaccg
     4801 ggaaagaaac ggaaagcaca tgaaaaacat caaaactcac accggactcc ttatccacaa
     4861 agaacaaacg cggcgcgttc gtcttcatga aacacccact gcctggtgcc acacgcaccg
     4921 ggaatgctac agcaaaacga ccggtcgccg ctgcggcagc cctgacagtc tcagcaggct
     4981 gattctgagc agt
//
LOCUS       DAZFJF010000226         4962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFJF010000226 DAZFJF010000000
VERSION     DAZFJF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.228"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            140..643
                     /gene="ftnB"
                     /locus_tag="ACHKAU_004868"
     CDS             140..643
                     /gene="ftnB"
                     /locus_tag="ACHKAU_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO5533769.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(722..973)
                     /gene="yecJ"
                     /locus_tag="ACHKAU_004869"
     CDS             complement(722..973)
                     /gene="yecJ"
                     /locus_tag="ACHKAU_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO5533770.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(1088..1174)
                     /gene="azuC"
                     /locus_tag="ACHKAU_004870"
     CDS             complement(1088..1174)
                     /gene="azuC"
                     /locus_tag="ACHKAU_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO5533771.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1437..1760
                     /gene="yecR"
                     /locus_tag="ACHKAU_004871"
     CDS             1437..1760
                     /gene="yecR"
                     /locus_tag="ACHKAU_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO5533772.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1931..2428
                     /gene="ftnA"
                     /locus_tag="ACHKAU_004872"
     CDS             1931..2428
                     /gene="ftnA"
                     /locus_tag="ACHKAU_004872"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO5533773.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2466..2705)
                     /gene="yecH"
                     /locus_tag="ACHKAU_004873"
     CDS             complement(2466..2705)
                     /gene="yecH"
                     /locus_tag="ACHKAU_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO5533774.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2896..4107
                     /gene="tyrP"
                     /locus_tag="ACHKAU_004874"
     CDS             2896..4107
                     /gene="tyrP"
                     /locus_tag="ACHKAU_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO5533775.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4169..4834)
                     /gene="yecA"
                     /locus_tag="ACHKAU_004875"
     CDS             complement(4169..4834)
                     /gene="yecA"
                     /locus_tag="ACHKAU_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO5533776.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT         1209 a         1203 c         1150 g         1400 t
ORIGIN      
        1 attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa
       61 ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct
      121 ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat
      181 gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca
      241 gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat
      301 gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga
      361 tgttcccggt gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata
      421 cgagcaacgt tctagtacgt tggcacagtt agccgatgaa gcgaaagaac tgaatgatga
      481 ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg gtctgttgct
      541 gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac
      601 cgaccaacat gttctgaatg tcgtgtcaca ccagctgcat tgatcatcat cggcgctaat
      661 gcattgcgcc gatgaaggtt ttgagaaacc gcagcctcat ctgtttgaag cagcggtttt
      721 tttaatggga ttcaccctgt ggggtaaact tgagttcaat aagcgcgatg gctttttgga
      781 ttgcctgcat ggtgaccggg tctgcggcgg cgggatggtt agtaaagtcg atattcttca
      841 gctgactgga cattttttca cgaacttcaa cgggcgcgat tacatcgaga acatccagaa
      901 tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga tcggcattca
      961 gcggctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt agcggcttca
     1021 catcttcagc gatagtcaca tccaccgtca tcaggacaca aaaaaacctg ccagagcagg
     1081 ttttttatta tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg caatagttat
     1141 tgaacatact tttcaggatt ttgcgcagtt tcatcgcggc actccgacca tttgttatac
     1201 aggtgttatt ctctttgcgc ttataatatg acaaccatca caaaaatcaa tctttatgtg
     1261 atacaaatca cataaatacc cctttaatgt tataaaaatg ataatcaaaa aacagccccc
     1321 ctatttctga cacctacaga tggcaagaaa tagcgcctgc caggcgtctt ttccggccat
     1381 tgtcgcagcg ctgtaacgcg taaaatagtg ctttctctta ctcttctggc tggaccatga
     1441 gacttctgat tctgactctt tcattaataa cgctggcggg gtgtacggtt actcgccagg
     1501 cccatgtgag cgaagttgac gcggcaacag gtattgtacg gttggtttat gaccaggctt
     1561 ttttgcagca tgcccatacc gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc
     1621 agcaggaagg ctatacccac gcggtcccct ttggtcagcc agtaggcaac tgtagccttt
     1681 ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc catcattccg
     1741 ccttccctgt ctttctctaa atattttaat cagcgagggg atcttcgctg attaaagaaa
     1801 tagcggtaat gcgttttatt cccattcgta tttttaataa ttgaagttta tattttacct
     1861 tttgcaaata ataaaataac aaattatagt gacgccacag caacaaatat aacctttgtg
     1921 gagcactatc atgctgaaac cagaaatgat tgaaaaactt aatgagcaga tgaacctgga
     1981 actgtactct tcactgcttt atcagcaaat gagcgcctgg tgcagctatc ataccttcga
     2041 aggtgctgcc gcgttcctgc gccgtcacgc ccaggaagag atgacgcata tgcagcgtct
     2101 gtttgattac ctgactgata ccggcaattt accgcgtatt aataccgttg aatctccgtt
     2161 tgctgaatat tcctcacttg atgaattatt ccaggaaacc tataaacacg aacaattaat
     2221 cacgcagaaa attaacgaac tggctcatgc tgcaatgacc aatcaggact acccaacatt
     2281 taatttcctg caatggtatg tttctgagca gcatgaagaa gagaaactgt tcaaatcgat
     2341 tattgataaa ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga
     2401 actctctacc ctcgacacac aaaactaatg ctctcaggcg gcaggttttc tgccgccgtt
     2461 tcaaatcagt gacggcaaat cttactctga tcggtcgaaa aaccttcttc tgaaggtata
     2521 aatttgcctt ttgctgccag aaacgctacc agctctcccg cagtcatccc ttctgccgag
     2581 caggtgtgaa aacgtgcctg ttcaccaaaa cgcgctttaa ttgctgcttc cagactggca
     2641 tgcgtatatt gctcgcctga ttcaatcatc atatttaaca cttcatgacc gtgaatagag
     2701 tccatcgtcc ctcctcaaaa aaagcctagc gtagcgattg ccgcttatga agactttgcg
     2761 ccagcgcagg actgaatgct ttttattgta catttatatt tacaccatat gtaacgtcgg
     2821 tttgacgaag cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc
     2881 aggacagaag aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc
     2941 acaattggcg caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt
     3001 acgttaatct tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg
     3061 gaggtgtacc agcatgttcc ggcagatacc ggtcttggca cgctggcaaa acgctatctg
     3121 ggacgctacg gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact
     3181 gcggcataca tcagcggtgc cggtgaattg ttggcctcca gcatcagcga ctggacaggt
     3241 atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt
     3301 tgtgttggaa catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt
     3361 tttctagtgg tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc
     3421 ctgccgttgc aacaggggct ggctctgtca gcaatcccgg tgatttttac ctcatttggt
     3481 tttcacggta gcgtgccgag tattgtcagc tatatggatg gcaacgttcg taagctacgc
     3541 tgggtgttta taaccggtag tgcgatcccc ctggtggcat atattttctg gcaggtggca
     3601 acgcttggca gcattgattc aacaaccttt atgggattgc tggctaatca tgctggatta
     3661 aacgggctgt tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcagtg
     3721 catttatttg ctgatttagc cctcgccacg tcatttctcg gcgttgcgtt aggcttattt
     3781 gattatctgg ctgatttatt tcagcgttca aataccgttg gtggacggtt gcaaactggt
     3841 gcaattacct ttctgccgcc gttggcgttt gcactgtttt atccacgagg atttgtgatg
     3901 gcgctgggtt acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcgctgttg
     3961 acctggcaaa gcagaaagca caatcctcag gcgggttacc gggtgaaagg tggtcgtccg
     4021 gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg
     4081 gcagggttgt taccagaagt ggggtgatca gatagcctca aattccttat tgggtgccag
     4141 aattaacgct gacacccaat ttgacctctt aatgcaggca gcactgctta aatttcttac
     4201 cactaccgca cgggcaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct
     4261 gaacagcttt ttcctgcgga tgcgccatcc agtacgcatg tagatcaagc gccgccagtc
     4321 gaatggcatc tacgctctct tcaaacgctt ctggcgacat tttctctacc cgctcgaagt
     4381 tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg ttttaacgaa tcgggcaacg
     4441 ttgaccagtc agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg
     4501 tcagctcgct gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt
     4561 cgttcagacg ctctgcggta tcggccatat gttgaaaagc cagattcata aagcgcgtca
     4621 tctctttctc tgacgcccag cgcggcacat agtcagcccc accccacacg gcaaccagcc
     4681 actgttccgg ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct
     4741 ccgccacatc aaggatggcg tggtcagtgt tgtatttggt cagaatatcg tccagccatt
     4801 ccaactcact ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc
     4861 gccagcacct atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgctgg
     4921 tgattaaaaa ttagggaggg tgtaacgaca agttgcaggc ac
//
LOCUS       DAZFJF010000227         4911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFJF010000227 DAZFJF010000000
VERSION     DAZFJF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.229"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..197
                     /locus_tag="ACHKAU_004876"
     CDS             <1..197
                     /locus_tag="ACHKAU_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5533777.1"
                     /translation="PYGVLDSKGFGVFLAMICATFIISIISYNFIEKPSVSIGHAIAR
                     RMQKKSDVSSIKTESVREAP"
     gene            complement(442..1599)
                     /gene="intS"
                     /locus_tag="ACHKAU_004877"
     CDS             complement(442..1599)
                     /gene="intS"
                     /locus_tag="ACHKAU_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO5533778.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(1761..1835)
                     /locus_tag="ACHKAU_004878"
     tRNA            complement(1761..1835)
                     /locus_tag="ACHKAU_004878"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1800..1802),aa:Arg,seq:cct)
     gene            complement(1911..2843)
                     /gene="yfdC"
                     /locus_tag="ACHKAU_004879"
     CDS             complement(1911..2843)
                     /gene="yfdC"
                     /locus_tag="ACHKAU_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO5533779.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3137..3892
                     /gene="mlaA"
                     /locus_tag="ACHKAU_004880"
     CDS             3137..3892
                     /gene="mlaA"
                     /locus_tag="ACHKAU_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO5533780.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4074..>4911)
                     /locus_tag="ACHKAU_004881"
     CDS             complement(4074..>4911)
                     /locus_tag="ACHKAU_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533781.1"
                     /translation="TYIDIGSNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIV
                     EKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYG
                     KDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNASAW
                     FESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDN
                     VKEIISNLFANKVDVDHPENKHKDFKDLEDNVEKRLQNRQAKYQNEINQLSAPGVNFD
                     DI"
BASE COUNT         1232 a         1124 c         1100 g         1455 t
ORIGIN      
        1 aaccgtacgg ggtattggat agtaaaggat ttggtgtttt tctagctatg atttgcgcta
       61 cttttattat atcaatcata tcttataact ttattgaaaa accatcagta tccatagggc
      121 atgccatcgc tcgtagaatg caaaaaaaat cagatgtatc ttcaattaaa actgaatccg
      181 ttagagaagc accttgataa atagagcaat agccagatag cacctaaccg atttcgtact
      241 catagggttg tcactttacc aaactgagag gaaatacact gataacagtt ggttatgaag
      301 tactgggtga ggtggatgtg aatggtattt tgatgcattt cattaacctg tacgcctaag
      361 aaagcaaccc tgaaatctaa gacacacaag ctttgcactg gattgcaagg ctttgtgcac
      421 ttcgatagtg gttaaggtgg ttcactccac cttttcatca agccagtccg cccaccattg
      481 catcatttct ctgcgcttat cgagatactg agcatggttg taaataccgc gcacagatcc
      541 gccgttggca tgtgccagtt gcacttcaat agcgtcagca ggccattcgt gctcgttcat
      601 aatcgtgctg aattcatgcc tgaatccgtg accgctttcc agaccctcat agccgatttg
      661 tttgatcaca agtagcaccg cgttctcgca gattggcttc ttcttatcgt tgcgcccggc
      721 aaaaacaaat tctgatactg gtttggtgat ggagcttaac gtagtgagaa gttcaaccac
      781 ctggtccgac atcgggacaa catgaatttt gcgtcccttc atcacattgg cgtcaatggt
      841 gataatcctg ttttcaaaat cgacgttctt ccattgcatg gaacgaagct cttttgttct
      901 tagggctgtg tagcgtaaaa ccttggtggc aatgagcgat acgatacttc ctgaaaatgt
      961 tgccagtgct ttattgaatg ccgggatctg gtctgcagga agaaacggga agttcttctt
     1021 gcggtatccc ctcatggcgt cagcaaggtc aggtgccggg ttatatttag ccctgccggt
     1081 gacaatagcg taacggaaaa cctcgccgca tcttctgcgg gctttatttg ctcgctccat
     1141 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
     1201 aatttcaagg ccgccgatga taggcaaaat gtcgtcatca aacatttttg caagttcagt
     1261 cgcataccct actgaccata cttgcttctt gtgctcgtac cattccttgt aaatcgcact
     1321 aaaggaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc caaccgagat
     1381 gtctttcctc gcagtccatg ccttatccct tgcctcctgc aaagtcatta gcggatattt
     1441 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
     1501 cccggataca ggaacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
     1561 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggagggtaa
     1621 aattatttta tccacttttt acccgtcatg gggtgcggct gtcaacgatc tgacgcgaac
     1681 catgacgaac tgtgaatcta cggaaggctt gatattcagg ggattttgcg gactggtacg
     1741 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
     1801 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
     1861 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
     1921 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
     1981 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
     2041 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
     2101 tgcagcgtgc cgttaaacac cagatagagg atttcaacag aaccgacaac gatgtgggtg
     2161 gtgtcaccca gtgcaataag ccaggtcatc aaaataatca ccacaatctt tgccgcaccc
     2221 gctgcaggaa acatccaaac catagtggcg atcagccagc cggaaatgat cgcgttggca
     2281 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     2341 gtttcttcat tgaagatagg catatattca aatgcccatg cagcaatacc tgtcccgaga
     2401 atattaccca gcagcacgac gccccataat cgcataagta agccgacgtt gctcattgtc
     2461 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
     2521 gccataatga cgataataaa accaaaggta taaccgagat tctccagtaa gaagctgcct
     2581 ggcactcctt ccagttcgac atgaaatatc ccttttgcca gtagcgaagc gcccatcgac
     2641 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttccag ctctttttca
     2701 ccatcctggc ggatatgctc atgaattgcc atcgcccggg aggggagtcg gtcttcatct
     2761 atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     2821 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     2881 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt
     2941 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     3001 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     3061 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     3121 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     3181 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     3241 caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt
     3301 cgctgtcgcc tggcgtgatt atgttccgca accggcgcgt aatggtttga gcaactttac
     3361 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     3421 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     3481 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     3541 tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag
     3601 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     3661 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca
     3721 gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     3781 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     3841 tccgaacgca caagcgattc aggatgattt aaaagatatt gattctgaat aagaaacaaa
     3901 taaaaaaggt gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta
     3961 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga tgttcgtcac
     4021 tgactatttc cgatattcag gcgttgaaaa atcaaaaaac tggaaaaata attttaaata
     4081 tcatcaaaat taacacctgg cgcagatagt tgattaattt cattttgata tttcgcctgg
     4141 cgattttgaa gtcttttttc aacgttatct tcgagatcct taaagtcttt gtgtttattt
     4201 tcaggatggt caacgtcaac tttgttagcg aataaattag aaattatttc ttttacattg
     4261 tcgcgtctaa attttgcatc gtccacttga aaattatatc ttattgctcg acctaaacgt
     4321 ttaaaaactt cgttaggttc tctcagtctt tccccgttga gctcgtttac aacctgatga
     4381 tcggaacctg ttgcaaatga ttctgccatg atagctttac tgctttcaaa ccacgctgaa
     4441 gcattcctgg ctttttcact acaatattgg gtgagactat caccattaat acgaattcct
     4501 ctttgaatgt ggtagtcgtc ctgatcgtag taaccgagag ataagtaatc atttccgttt
     4561 ttatttttaa ttacaatagc gagagagtcg ccttttttat ctttaccgta ccaggtaaat
     4621 aagtccgttg gttcgctatt cccgctctca tcaaactccg ttaccgtatg tctgagtttg
     4681 tgtgatatct tatcgaagat aaaaccagga tctccatcat atatttcttt taatgcgctg
     4741 aagatcattt ccttgttatt gatatcatga gtcttctcaa ctatgttgtc attaatgata
     4801 gtacggacga gttctttgtc tttatagttc gcaggtaatt ctattggttc ttggttgtcg
     4861 ataccaatag taatatggtc tgtatcattg gagccaatat caatataagt a
//
LOCUS       DAZFJF010000228         4911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFJF010000228 DAZFJF010000000
VERSION     DAZFJF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.230"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            115..255
                     /locus_tag="ACHKAU_004882"
     CDS             115..255
                     /locus_tag="ACHKAU_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO5533782.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDVLNQPGVPK
                     NG"
     gene            248..361
                     /gene="kil"
                     /locus_tag="ACHKAU_004883"
     CDS             248..361
                     /gene="kil"
                     /locus_tag="ACHKAU_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5533783.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVEAYRKWRSK"
     gene            358..546
                     /locus_tag="ACHKAU_004884"
     CDS             358..546
                     /locus_tag="ACHKAU_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533784.1"
                     /translation="MIPVELAKTPELSRLKREYHIAEARYWRKAGDKSKKQLCLWQAQ
                     RERMNEREFLSSPSELPF"
     gene            555..1235
                     /locus_tag="ACHKAU_004885"
     CDS             555..1235
                     /locus_tag="ACHKAU_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5533785.1"
                     /translation="MGTAILILGESGTGKSTSMRNINPEEAILIKPIGKPLPFKSKNW
                     LAWDARAKKGTVVTTDKWDVIVAVIKRAHEYGKRIVIVDDFQYVMSNEFMRRSEEKSF
                     DKFTEIGRHAWEVIKAAQDAPDDLRVYFLAHTEETPMGRVKMKTIGKMLDEKITVEGM
                     FTIVLRTLTRDDQFFFTTKNNGADTVKSPMGMFDSNEIDNDLSFVDATVCDYYGINNV
                     HQIKENAA"
     gene            1232..1819
                     /locus_tag="ACHKAU_004886"
     CDS             1232..1819
                     /locus_tag="ACHKAU_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFO5533786.1"
                     /translation="MSNVIFTYNEEAALTAGQGGFINETGAYIITITEAELKQSEKGA
                     KFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGANVVHAMMGCAGIGQLTQHMVSASK
                     FVAPEFHGKKIGLVLQKVLTTNKKTGADSYQMEIRIPFIAQTGQTLKEKAEGKQPETI
                     ANMVASLKDKDNRSKNVSHNHADDYGYSQNDYPPF"
     gene            1843..2139
                     /locus_tag="ACHKAU_004887"
     CDS             1843..2139
                     /locus_tag="ACHKAU_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO5533787.1"
                     /translation="MPAPLYGADDPRNCSGSSKAEVLGNIKNNFDAFLALTPETKAER
                     MYRRDIQLALKQEKDRTNETAMRPLRKATIDKFPEYIDPRLRNYRSRYGAISNN"
     gene            2150..2314
                     /locus_tag="ACHKAU_004888"
     CDS             2150..2314
                     /locus_tag="ACHKAU_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO5533788.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFCSVNDNKY
                     LNAMWRSGKK"
     gene            2311..2940
                     /locus_tag="ACHKAU_004889"
     CDS             2311..2940
                     /locus_tag="ACHKAU_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533789.1"
                     /translation="MKQMTLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALSEDQQKAIESIKQADSAVKLAHEKFSALAAENELARKAVQALCDVVGDNTEVIAEV
                     VGRDGVLVILEAMKATGNMPATDAFLAEIRAEARNEGINYTASRLAAAFNHGFINKSL
                     REVFDVTRMILSAKEELANESHPIDGLSGEYAEKSLEEWAERLRKGGNQ"
     gene            2937..3344
                     /locus_tag="ACHKAU_004890"
     CDS             2937..3344
                     /locus_tag="ACHKAU_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5533790.1"
                     /translation="MSKIDYQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSYQPIAEIDCKPVRDFVAKANPATVLELLDELEAAKKRIAELEAREILLPERSSMLH
                     RTDFHDDYQTVMAYKVSEVIDAIRATGIRIKGE"
     gene            3346..3537
                     /locus_tag="ACHKAU_004891"
     CDS             3346..3537
                     /locus_tag="ACHKAU_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533791.1"
                     /translation="MNGQISIVRPGACDDSEIHMIIRLARGKTITVLTTPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            3540..3998
                     /locus_tag="ACHKAU_004892"
     CDS             3540..3998
                     /locus_tag="ACHKAU_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533792.1"
                     /translation="MTTFTKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AEPVMFCISGQNVDSEEHVSTSKAVVDAWVEEWNQVDGSPGEPLYKTMPLYCHAALPA
                     PVVPEGLVKAVRFYEQVKNENPPVGTGAWKDAVDWVLEEACQAVNTDIKG"
     gene            4004..4201
                     /locus_tag="ACHKAU_004893"
     CDS             4004..4201
                     /locus_tag="ACHKAU_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533793.1"
                     /translation="MATNNRKAKMLISRVYRLCYPSQWLRVSNRRVVLFSFSGIAREG
                     VKDKRSAAQNRWKKHLRTKGE"
     gene            4203..4381
                     /locus_tag="ACHKAU_004894"
                     /pseudo
     CDS             4203..4381
                     /locus_tag="ACHKAU_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4715..>4911
                     /locus_tag="ACHKAU_004895"
     CDS             4715..>4911
                     /locus_tag="ACHKAU_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5533794.1"
                     /translation="MITITKERLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAKIIAHYPLGVDVGKQK"
BASE COUNT         1505 a         1021 c         1230 g         1155 t
ORIGIN      
        1 gttctgatta ttggaaatct tctttgccct ccagtgcgtg agggcctttt tatatgcata
       61 ccaataacgc ttcactcgag gcgtttttcg ttatgtataa ataaggagca caccatgcaa
      121 tatgccattg cagggtggcc tgttgctggc tgcccttccg aatctttact tgaacgaatc
      181 acccgtaaat tacgtgacgg atggaagcga ctcatcgacg tacttaatca gccaggagtt
      241 cccaaaaatg gataaaacac ttatggctat ccagactaaa ttcactatcg ccacttttat
      301 tggcgatgaa aagatgtttc gtgaagccgt agaagcctac agaaaatgga ggtcaaaatg
      361 attccggtag aactggcgaa aaccccagag ttaagtcgat taaaaagaga gtatcacatt
      421 gctgaggctc gttactggcg taaagcggga gataaatcaa agaaacaact ttgcttatgg
      481 caggcacaaa gagagcgcat gaatgagcgc gaatttcttt cctccccatc cgaattacca
      541 ttctgaggtg aattatggga actgcgatat taatactcgg tgagtctggc accggaaaat
      601 caaccagcat gagaaatatc aatccagagg aagcaatact tataaaacca ataggcaagc
      661 cgctaccatt taaatcaaaa aactggcttg catgggatgc cagagcaaaa aaaggaaccg
      721 tagttaccac tgacaaatgg gacgtaatag ttgctgtaat taagcgtgct cacgaatacg
      781 gaaaaagaat cgttattgtt gatgacttcc agtatgtgat gagcaatgag tttatgcgcc
      841 gctcagaaga aaaatcgttt gataaattta ctgagatagg ccgccacgca tgggaggtca
      901 ttaaggctgc acaggatgcg cctgatgacc tgagagtcta ttttcttgcg cacaccgaag
      961 aaacccctat ggggcgcgtg aaaatgaaga ctatcggcaa aatgctggac gagaaaatca
     1021 ctgtcgaagg catgtttact atagttcttc gcactcttac ccgcgatgac cagttctttt
     1081 tcaccacgaa aaacaacggt gcagacactg ttaaatcccc aatgggaatg tttgattcca
     1141 atgagattga taacgatctc tctttcgtcg atgccactgt ttgtgattac tacggcatca
     1201 ataatgttca tcaaattaag gaaaacgccg catgagcaac gtaattttta cttataacga
     1261 agaagcagca ctgaccgcag ggcaaggtgg ttttattaac gaaactggcg cttacataat
     1321 taccattact gaagcagaac tcaagcaatc agaaaaagga gccaaattta ttgagttttc
     1381 tggagaatcc gacgacggac gtaaaatcca gtatcttagc gtctgtgttc agaaaaatga
     1441 cggcacggaa aacaaatttg gcgcaaatgt cgttcacgcc atgatggggt gtgccgggat
     1501 tggacaatta acgcaacata tggtttccgc cagtaaattt gttgcacctg aatttcatgg
     1561 aaagaaaatc gggttagtgc tccagaaagt attaaccaca aacaaaaaga ctggcgcaga
     1621 cagctaccag atggaaatac gcatcccgtt tattgcacaa acaggtcaaa cccttaaaga
     1681 aaaggcagaa ggcaagcaac cagaaactat cgccaacatg gttgccagcc tcaaagataa
     1741 agacaatcgc tctaaaaacg taagccataa tcatgcagat gattatggtt acagccagaa
     1801 cgattaccct cctttctgat tactgaaaat aaggctccca ttatgccagc gcctctgtat
     1861 ggtgcggatg acccgcgcaa ctgctccggt agctccaagg cggaggtgct gggaaatatc
     1921 aaaaacaatt tcgacgcgtt tcttgctctg acaccagaaa caaaagcaga acggatgtac
     1981 cgacgcgata tacaactcgc gctaaaacag gagaaggacc gaacaaacga aacagcaatg
     2041 agaccgttgc gaaaagcgac aatagacaaa ttccctgaat atatcgaccc gcgcctgcgt
     2101 aattaccgct cacgctatgg cgctatcagt aataactgag gaatttacca tgagaggact
     2161 tgcatacaat cccggcattc ttccggcaga aatgattatt cgccaacgcg taaagccaat
     2221 gccatcgaga gaggaattgc ttaagcgaaa gagtttctgc tctgttaatg ataacaaata
     2281 tctgaatgcg atgtggcgga gtgggaagaa atgaaacaaa tgacactaat tgagatggat
     2341 ggatttctga aaggtaaatg catcccatgt gatttaaagg ttaacgaaac aaacgctgaa
     2401 tatcttgtgc gtaaatttgc tgaagcggag gccaagattt cggctctgtc cgaagaccag
     2461 cagaaagcga ttgagtcaat taagcaggct gattcggctg ttaagttggc acacgagaag
     2521 ttttcggcgc ttgcggcgga gaatgagctg gctcgtaagg cagttcaggc attatgcgat
     2581 gttgttggcg acaacaccga ggttatcgct gaggtggttg ggcgagatgg cgttctggtt
     2641 attttggagg ccatgaaggc aacaggaaat atgccagcca ccgatgcttt tctggctgaa
     2701 attcgtgcgg aggctcgcaa cgaggggatt aactataccg caagccgtct tgctgctgct
     2761 ttcaaccacg gatttatcaa taagtcttta cgtgaagttt tcgacgttac gcgcatgatt
     2821 ctgtcagcaa aagaagagtt agctaatgaa tcgcatccga ttgatggcct gtccggtgaa
     2881 tatgcagaga aatcccttga agaatgggcg gaacggcttc gcaaaggagg caaccagtga
     2941 gcaagattga ctatcaggca ctgcgtgaag cggcagagaa agccggtgaa gataagtggc
     3001 aggctaaaaa aataaatggt gattttttcg ttattcgtca cggtagttat acaagacagc
     3061 atggctacac atcgtatcaa cccattgcgg agattgattg taagccagtc cgggattttg
     3121 ttgccaaggc taatccggct accgtgctgg aattactgga tgaactggaa gcagcaaaaa
     3181 agcgcattgc agaactggaa gcgcgggaaa tactgctccc ggaacgtagc agcatgcttc
     3241 atcgaacaga ttttcacgat gattaccaaa cggtaatggc atacaaagtt tctgaagtca
     3301 tcgatgcaat ccgcgctact ggcattcgca tcaaaggaga gtgagatgaa cggacaaatc
     3361 tcaattgttc gaccaggagc atgtgacgat agcgagatac acatgattat tcgtctggcg
     3421 agggggaaaa caataactgt tctcactact ccagaaaatc tcgcattagc attaacagga
     3481 aagtcagacc tgccagtaga gctaaagctg cgaaatgttg agattaaggt gaaatagcta
     3541 tgaccacttt taccaaagat cgactgctga caatccagca ttggcgcgaa acatacggac
     3601 cgggtagcaa cgttgtgctt ccagcagaag aagcggaaga gctggcacgg attgcgctgg
     3661 catcactggc agcagagcca gttatgttct gtatatcagg acaaaatgta gattcagaag
     3721 aacatgtatc aaccagcaaa gcggttgttg atgcctgggt tgaagaatgg aatcaggttg
     3781 acggaagtcc tggcgaacca ctgtacaaaa ctatgccact ctactgtcac gctgccttac
     3841 cagcgccggt agtgcctgaa ggactggtta aagcggtgcg tttttatgag caggtaaaaa
     3901 atgagaatcc gccagttggg actggtgcgt ggaaagacgc tgttgactgg gtgctcgaag
     3961 aggcttgcca ggctgtaaat acagacatca aaggatgaac gatatggcaa ccaacaatcg
     4021 taaagccaag atgctaattt cccgtgtata cagactttgc taccccagcc agtggttgag
     4081 agttagcaat cgccgtgtgg tgttgttctc attttctgga attgccagag agggagtcaa
     4141 agataagcgc agcgcggcgc aaaaccgctg gaaaaaacat ttgcgcacta aaggagagtg
     4201 atatggcgtt aacacaccgc gaactctgtc agattgcgta caagttcctt aagcgcaacg
     4261 ggttcaaggt ttgttttcat gaccgcttta tagctgtaac cagtaccgga gaacagccag
     4321 atgctatggg gttcagaaat tcagcatcat gcctgataga ggcgaagtgt tctcgtgctg
     4381 acttgttggc agatagaaat aagcgtttcc gtaaaaatcc ctcacttggc atgggcgact
     4441 ggcgattctt tattagtgag ccgggaatta tttcagttga ggatttacct cccggctggg
     4501 gattacttca cgttgttaac ggaagagtac ggaaagtaca tgggtggccc aagggtaatt
     4561 gctgttgggg taatcctgac gataagccat ttactggaaa taagcaggtt gaatgcgatt
     4621 acatgttatc tgcattaagg cgcatggagt tgagagggca ccttaatgaa atatatgacg
     4681 gtgtaattgt taataagaaa gaaggaaacg cggcatgatc actattacca aagagcgact
     4741 gctgacaatc cagcattggc gcgaaacata cggaccgggt agcaacgttg tgcttccagc
     4801 agaagaagcg gaagagctgg cacggattgc gctggcatcg ctggaagcag agccggtggc
     4861 aaagattata gctcattacc cattaggggt tgacgtaggc aaacaaaaat t
//
LOCUS       DAZFJF010000229         4890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFJF010000229 DAZFJF010000000
VERSION     DAZFJF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.231"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..596)
                     /locus_tag="ACHKAU_004896"
     CDS             complement(<1..596)
                     /locus_tag="ACHKAU_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5533795.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHKAU_004897"
     tRNA            complement(711..786)
                     /locus_tag="ACHKAU_004897"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHKAU_004898"
     tRNA            complement(793..867)
                     /locus_tag="ACHKAU_004898"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(984..1068)
                     /locus_tag="ACHKAU_004899"
     tRNA            complement(984..1068)
                     /locus_tag="ACHKAU_004899"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Tyr,seq:gta)
     gene            complement(1077..1152)
                     /locus_tag="ACHKAU_004900"
     tRNA            complement(1077..1152)
                     /locus_tag="ACHKAU_004900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Thr,seq:tgt)
     gene            1514..2464
                     /gene="coaA"
                     /locus_tag="ACHKAU_004901"
     CDS             1514..2464
                     /gene="coaA"
                     /locus_tag="ACHKAU_004901"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO5533796.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHKAU_004902"
     CDS             complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHKAU_004902"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO5533797.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGSSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHKAU_004903"
     CDS             complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHKAU_004903"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO5533798.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4786..>4890)
                     /gene="rrf"
                     /locus_tag="ACHKAU_004904"
     rRNA            complement(4786..>4890)
                     /gene="rrf"
                     /locus_tag="ACHKAU_004904"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1230 a         1252 c         1117 g         1291 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcatactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaagat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
     1021 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
     1081 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
     1141 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
     1201 aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga aacagccgaa
     1261 cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa
     1321 accataattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1381 ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1441 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1501 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1561 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1621 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1681 acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1741 ggaacagttt cttggtacta acgggcaacg cattccttac attatcagta ttgctggcag
     1801 tgtcgcggtg gggaaaagca caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1861 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1921 gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct
     1981 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc
     2041 gcatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc ctgatatttt
     2101 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     2161 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     2221 tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta ccgacccgga
     2281 ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac
     2341 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg
     2401 cgccagttta atcctgacga aaagtgctaa tcatgcggta gaagaggtca gactacgcaa
     2461 ataatttgca ggggagcgaa tactcccctt tcttattttt ctgcactacg cagggatatt
     2521 tcaccgccca tccagggttt tattattcca tcctgctcaa gtaataaagc cccctgttta
     2581 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2641 ttaataaaat tatccagctt ttcccagcgc gacagataag gtgccaatcc ttcttgttcg
     2701 aagagttcca acgcagcacg taattcacgt attagcatgg ccgccaacgt attacgatcg
     2761 agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca
     2821 cggcgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc gccagttttg
     2881 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2941 catttgacac gaactttatc tgctcccagc ttgcgtaata cttccgccat cacgataccg
     3001 ataaccagac ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca gaacatcgac
     3061 aaatataagt ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc acggccagcc
     3121 tgctggtatt ctgcaacaca ggcatcgccc gatttaagct ctccgatacg atcaagaagg
     3181 tactgattcg tggagtcaat aactggcagc acggctacac tactgccatc cagctgaccc
     3241 aatatctgtt cagcattaag taactggatg ggctcaggca ggctgtatcc tttacccgga
     3301 acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3361 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3421 agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3481 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3541 ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt
     3601 cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt
     3661 ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg
     3721 ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa
     3781 cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg
     3841 tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac
     3901 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3961 ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     4021 ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat
     4081 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     4141 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     4201 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4261 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca
     4321 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4381 actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4441 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4501 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4561 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     4621 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
     4681 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4741 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4801 actctcgcat ggggaaaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc
     4861 ggcatggggt caggtgggac caccgcgcta
//
LOCUS       DAZFJF010000230         4796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFJF010000230 DAZFJF010000000
VERSION     DAZFJF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.232"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..789
                     /gene="kdgR"
                     /locus_tag="ACHKAU_004905"
     CDS             <1..789
                     /gene="kdgR"
                     /locus_tag="ACHKAU_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO5533799.1"
                     /translation="ANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMSK
                     STVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISRL
                     TKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEVK
                     QILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFGV
                     VIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(932..1171)
                     /locus_tag="ACHKAU_004906"
     CDS             complement(932..1171)
                     /locus_tag="ACHKAU_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO5533800.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            1330..1473
                     /gene="mgrB"
                     /locus_tag="ACHKAU_004907"
     CDS             1330..1473
                     /gene="mgrB"
                     /locus_tag="ACHKAU_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO5533801.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            1548..1835
                     /locus_tag="ACHKAU_004908"
     CDS             1548..1835
                     /locus_tag="ACHKAU_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO5533802.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            2504..2647
                     /locus_tag="ACHKAU_004909"
     CDS             2504..2647
                     /locus_tag="ACHKAU_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO5533803.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            2660..2869
                     /gene="cspE"
                     /locus_tag="ACHKAU_004910"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             2660..2869
                     /gene="cspE"
                     /locus_tag="ACHKAU_004910"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5533804.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            3035..3844
                     /gene="rlmA"
                     /locus_tag="ACHKAU_004911"
     CDS             3035..3844
                     /gene="rlmA"
                     /locus_tag="ACHKAU_004911"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO5533805.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(3841..4407)
                     /gene="mntP"
                     /locus_tag="ACHKAU_004912"
     CDS             complement(3841..4407)
                     /gene="mntP"
                     /locus_tag="ACHKAU_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO5533806.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         1294 a         1047 c         1131 g         1324 t
ORIGIN      
        1 gctaacgcag atctggataa acagcctgat tctgtatctt ccgtgctaaa agtttttggc
       61 attttgcagg cgctgggtga agagcgcgaa atagggataa ccgagctgtc gcagcgcgtc
      121 atgatgtcaa aaagcaccgt ttatcgcttt ttacagacca tgaaaacctt aggttatgtg
      181 gcgcaggaag gggagtcgga gaaatattcc ctgaccctga aattgtttga actgggcgct
      241 cgcgcgttac aaaacgtcga tttaattcgt agcgcagata tccagatgcg tgagatctcc
      301 cgcctgacca aagaaactat ccacctcggc gcactggacg aagacagtat tgtttacatt
      361 cacaaaattg actctatgta caatttgcgc atgtattcac ggattgggcg tcgtaatccg
      421 ctgtacagca ccgcgattgg taaggtactg ctggcatggc gcgatctcga tgaagtgaag
      481 caaattcttg agggcgtgga gtataaacgc agtaccgagc ggaccatcac cagtacagaa
      541 gcgttattac ccgttctgga ccaggtgcgc gagcaggggt atggcgaaga taacgaagag
      601 caggaagaag ggctgcgatg cattgcggta ccggtatttg atcgctttgg cgtggtcatt
      661 gccggtttga gcatctcctt cccgacgttg cgtttctctg aagagcgttt acaggaatat
      721 gtcgcaatgt tgcataccgc agcgcgcaaa atttctgccc aaatgggtta tcacgactat
      781 ccgttctgat gagagtaaga acctgtcgga atatcaaaca gacaggttct ttatttagca
      841 tgagaaaaat aaagttgaag gtgcgttata ttaaacgcgc ttgctataag aatattttac
      901 tcatgagtga gaatctggtt atttattacc cttaaccatt atcgaccacg atattgcttt
      961 tacgtaacag tgggcaatct gttatcccca agaaaccact tttagtgtgc aagtattgta
     1021 ccgtgctggt gcctctggca gtcagatagg tacattgcaa acctaatcct gcggcattct
     1081 ctttgcttcc aatcaaaacg ccatatccgc tgagtaataa tcctatccat accagtgcta
     1141 tcagcataac tgtgcgaatg atgaatcgca ttacaacctc ttctcttttt atgttcgctt
     1201 aatcgtagcg gcaatatgcg ctgaagcaag ctactcattc cgaaaaagca cgaatatcga
     1261 catagttagg cgctgtttaa ctaacgcatg ctagtttaat gacataaggt aggtgaagcg
     1321 gagattggag tgaaaaagtt tcgatgggtc gttctggttg tcgtggtgtt ggcttgcttg
     1381 ctgctttggg cgcaggtatt caacatgatg tgcgatcagg atgtacaatt tttcagcgga
     1441 atttgtgcca ttaaccagtt tatcccgtgg tgatatcatt ttttcagcac tagattttct
     1501 tcctctccag cagtggtaga atagcgcctc tactcagagg tggtgaaatg aacgaagttg
     1561 taaattcagg cgtgatgaac attgcgtctt tggttgtatc ggtggtggtt cttcttatcg
     1621 ggctcatctt gtggtttttt atcaatcgtg ccagttctcg gactaacgaa cagattgaac
     1681 tgcttgaggc gttgctggat cagcaaaaac gtcaaaatgc actgttacgt cgtttgtgcg
     1741 aagcaaacga acctgagaaa gcagataaaa agaccgttga gagtcaaaaa tcggttgaag
     1801 acgaagatat tattcgtctg gtcgccgaac gataatcctt cctcaatctt gagtaacccc
     1861 gtcgttgaag ggttactcaa ttcatcctga ttatacgcac tgccatggtg caactgcttc
     1921 aaattaaaac tctggtctga cttcaaataa ttatcatatt atttatttgc gtaacgggat
     1981 atcagaaaaa gatattttcc tcattaattt attaggatgt ttacatcgga tttgtgatta
     2041 agcgtggtat tatttattag cgaaacgttt ctctcttgat tttttgctca ttcatcaatt
     2101 tttcttattt taaattttca atccctttgg ggattgactt ctctttaggg taattgatag
     2161 ccgttaactg actgttttat gagaaaaagt gatataactt tttattcatt gcatagcaaa
     2221 aaatgtgata ttgcacgcac tatgtaataa cttctcccac tggcctggaa caactgaact
     2281 tattgaacta tgttagaaaa tacgccagtt taagtatctg cctgaactgg caaggttaag
     2341 cacaatgata tatcggcgcg tattccgttg cataagtgtg caaaaaaagt ggaagacgta
     2401 tcgagatttg tgcgtctgat cgagacatgt ttaaaaatgg cttgccataa ttaacgttgt
     2461 atgtgataac agatttcggg ttaaacgagg tacagttctg tttatgtgtg gcattttcag
     2521 taaagaagtc ctgagtaaac acgttgacgt tgaataccgc ttctctgccg agccttatat
     2581 tggtgcctca tgcagtaatg tgtcagtttt atctatgtta tgcctgcggg cgaagaaaac
     2641 aatctaagga atttttcaaa tggcaaagat taaaggtcag gttaagtggt tcaacgagtc
     2701 taaaggtttt ggcttcatta ctccggctga tggcagcaaa gatgtgttcg tacacttctc
     2761 cgctatccag ggtaatggct tcaaaactct ggctgaaggt cagaacgttg agttcgaaat
     2821 tcaggacggc cagaaaggtc cggcagctgt taacgtaaca gctatctgat cgaatccact
     2881 gatctgaagt gtgaatacgc ttcaatctcg ctataaagcc tcgtcgaatg cgaggctttt
     2941 tactatgctt tatcttcgct cctggcgttc ggatatttgc ccgccgcgtg attcgcgtta
     3001 cacttgcggc ctttagtatc ctgccggagt tgtcatgtcg ttttcctgtc cactttgcca
     3061 tcagcctctt tcgcgtgaaa aaaacagcta tatctgtccc cagcgacatc agtttgatat
     3121 ggcgaaagaa gggtatgtca atctgctgcc cgttcagcat aaacggtctc gtgatccggg
     3181 cgacagcgcg gaaatgatac aagcacgccg cgcattctta gatgccggac attatcagcc
     3241 gctgcgtgat gcaattgtcg cccaactgcg ggaacggctt gatgaaaagg ccacggcggt
     3301 gctggatatt ggctgtggtg aagggtatta cacacacgca tttgccgatg tgttgcccga
     3361 aatcaccacg tttggcctgg atgtctcgaa ggtagcgata aaagcggcgg cgaaacgcta
     3421 tccgcaggtc actctttgtg tcgcttccag ccaccgtttg ccgttttccg ataccagtat
     3481 ggacgccata atacgtattt acgcgccgtg taaagcagaa gaattagcac gagtagtgaa
     3541 gcccggcggc tgggtcatta ctgccacgcc gggaccgcga catttgatgg agctgaaggg
     3601 gctgatttac aatgaagtac atcttcatgc acctcatgca gaacaactgg aaggttttac
     3661 attacagcag agtgatgagt tgtgttatcc gatgcgtctt cgcggtgatg aagccgtcgc
     3721 attattgcag atgacgccgt ttgcctggcg tgcgaagcca gaagtctggc aaacactggc
     3781 agcaaaagaa gtgttcgact gccagacgga ctttaatatt cacctctggc agcgttctta
     3841 ttaaccgtgg aagtgcgtcc agaggatctg gacgccgatg ccgatcagca ccagcccgcc
     3901 gagaatttcc gcttttttcc caataattga gccgataaag cgaccaacca tcatccctaa
     3961 tgtcgacata atcaaggttg cacaaccaat ggccaatgcg gtcgcgataa tgttgacctg
     4021 caggaaagca agaccaacac ccacagccat ggcatccagg ctggtggcaa tcgcggtggt
     4081 taccagtagc cagaaaccgt gccgacggcg cggctcttca tcttcatcat ctgcgccacg
     4141 aaaaccctca ataatcattc gcccgccgag gaatatcagc agcacaaacg caatccagtg
     4201 gttccattca aggacaaacc ggctggctaa catgcccatt ccccagccga tcagcggcgt
     4261 cagggtttcg acggcaccaa aaataaggcc ggttcgcaat gcttcagaaa atttcggttt
     4321 atggagggtg gcacctttac cgattgatgc agcaaatgca tccatcgaca taccaaacgc
     4381 aagaagaaca gtagcagtga tattcatgac aatgtcctga ccggggtatc cataaaacac
     4441 atcatcgccc ccagcaaaca tgcatacatg aacaacaagc cgcgatggtt ttcgcgttca
     4501 tgcaggcata gcgattgatg tgcatatggt ctcgcctgac tgaaagtatt cagtccgtac
     4561 gtgccacgtt ttgcaacgag tatgttgaca cgtacatttc cggaatctgg aaatcggcta
     4621 ctccccaaat gagggcgcaa ccttaacata ttttgagaat ataaaacaac tgttggaata
     4681 tattatttca ttgctttatt gataacgatt tacatttaga tttatatatc aatgaaacgt
     4741 taaaataaat aacaaataag ggaagaggta atatagccta agctatgtct gattat
//
LOCUS       DAZFJF010000231         4776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFJF010000231 DAZFJF010000000
VERSION     DAZFJF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.233"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..851
                     /locus_tag="ACHKAU_004913"
                     /pseudo
     CDS             1..851
                     /locus_tag="ACHKAU_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(891..1183)
                     /locus_tag="ACHKAU_004914"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="ACHKAU_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2884)
                     /locus_tag="ACHKAU_004915"
                     /pseudo
     CDS             complement(1263..2884)
                     /locus_tag="ACHKAU_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3168..3578)
                     /locus_tag="ACHKAU_004916"
     CDS             complement(3168..3578)
                     /locus_tag="ACHKAU_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO5533807.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            3764..4732
                     /locus_tag="ACHKAU_004917"
     CDS             3764..4732
                     /locus_tag="ACHKAU_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5533808.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
BASE COUNT         1178 a         1252 c         1155 g         1191 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtagg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc ggacgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgct
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgattcagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct aggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caaatccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc attactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggact cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgtgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cgggatgcat aattttcttc gcccctgatt
     1621 attctccagg gcatcaaccc gcggcttacc cggaacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggttccagcc tttacgccat ttggcaacag tatccctgac gatggtttca
     1741 ctgcctgtcc ccccccctgg gccaccattt cccgccatat ccggctggca ttataattac
     1801 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     1861 gccgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcactcgcg
     1921 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc agggccataa
     1981 cctctgtcca atgctgatgc cgttttttgc gggtttgctg tttaaggcgt tccggacgac
     2041 ggagcgatgc tacaggcaca gatatttctg gctcaggtga tttcttcagt gataactctc
     2101 tgacaacaag acgtatcaga ggcatatgtc tgtacatcat tcgttcaggc tcatcaccaa
     2161 tattttttag caggtgccat cgatcggcca cctgtctggc ctgaggtgca ccttcacgtg
     2221 ctgctgtcgc atagactcca ctgcgatcac gcgagacaac ctgtatttcc ggatattttc
     2281 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     2341 tatcaagatt gactattaac ataccgcagc agtggccccg atgccacgcc cactcatcaa
     2401 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagaa
     2461 gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca gcctcacctc
     2521 cagctattaa tcccagttga cgttgtaaat tttgtaacgc ctgtgaagac tgttggtgtg
     2581 aaccggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagct gggttacggc
     2641 agtaccagtg gcggacggaa aatacaagcc atagcgtctg tcctgatcag ggtagatgct
     2701 gtatccggcg ccgacaacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     2761 gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta ataatttgtc
     2821 gactgcatcg ccatccgcaa ggtaactgta gaagtgtctt aagagagtgc attgggttgc
     2881 ccataactta aaacagctta gatctttgtt cgccagaaag tttcatccat tttcttccac
     2941 ccaggttgcg gaagaaccac ttttcatccg tcgttgacag atgtgaagta tgcgggagaa
     3001 aaagtgaata tctaactgta ataactcaga ccggtacgag tactcggggc gtctacaatc
     3061 attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac gcagcactcc
     3121 cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca gttcctatcc
     3181 ttccagttgc cttcctgcag ccccccctcc ctgctgtata ccccagtacc acaacgcggg
     3241 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     3301 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     3361 gggtcaagcc aggtgcatcg cagcaggatt ttcgcagcaa cagtcatctg gtcatgttca
     3421 aaccacggcg gttgtccgtc atttccccgc ttaagtgtta acggacaacc acaggacaca
     3481 cagcacaaaa cgtcctgtcg gttgtgcttc gcggcttctt tcgccgtaac aaaatggcct
     3541 ttgctgtcat tcgccatata gcagttcaga attcgcatat ctcctccttg tatcttgaca
     3601 attggttaaa ataactgtga ttagatttgg caaggtgtat gcagcccgat ccctcaaggg
     3661 gttcatagcc cgtagctcag cgaatggcgc atacactgag ccactcatct gaaacccgaa
     3721 cagttgtcag tacgccataa cgcatttgca aggatgggta atcatgagtc agccaggttc
     3781 actttgtgtg gatatcgatg tttcgaaagc gatactggat attgccgcca gcagcgctat
     3841 tgaacaattt tctgtcggca atgacagtga tggctttgac gccatcattg ctgagttgag
     3901 aatgtacgcc attgcgctgg ttttaatgaa agccactgga ggacttgaaa ccgccatagt
     3961 ctgtgcgctt caggcagaag gttttgagct tgccgtggtt aaccccagac aagctcgtga
     4021 ctttgcccgc gccatgggat acctcgcgaa aactgatagt attgatgcca gagtgctttc
     4081 ccagatggct gaggttctca accggcatcc ggagcgggaa cgctttatca gagcactgcc
     4141 ggatactgaa tgtcaggttc tcactgcaat ggtagttcgt aggcggcagt tgattacgat
     4201 gctggttgcc gagagaaacc ggttgcaccc tgctcatcca ttgaacagga aaagcattaa
     4261 tatcatcatc aaagcactgg aagacgagct tgcgcgacta gatgctgaca tgaacagcca
     4321 tatccatagc cacttcaaag agcttgctaa gcgactgagc agtattaaag gtgttggcac
     4381 catgacggct gctgcgctgc tggcggaagt acccgaacag ggtaacctct ccaggcgtga
     4441 aatcagtgtg cttgtcggta tggttccagt aaaccgggac tcgggcacga tgagagggcg
     4501 acgaaccatc tttggcggca gggctggtgt gcgaacagcc ctgtacatgg cagcgcttgt
     4561 cgcaacccgc tttaacccgg taataaaggt gttttacatg cgactgcttg ccgcaggaaa
     4621 acccaaaaaa gtggcgctgg ttacctgtat acgcaagctg ctgactatcc tgaacgcaat
     4681 gctcagaaag aacgaagaat ggaatgaatc gtaccatcac gttgctccat aatttttacg
     4741 ttcaagacag ttgctgagct gtccggggaa gactga
//
LOCUS       DAZFJF010000232         4740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFJF010000232 DAZFJF010000000
VERSION     DAZFJF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.234"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1315
                     /locus_tag="ACHKAU_004918"
     CDS             <1..1315
                     /locus_tag="ACHKAU_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5533809.1"
                     /translation="NYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQVAY
                     NKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSANI
                     MQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEE
                     ERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGI
                     YVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITN
                     QLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETE
                     LQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVG
                     QGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1328..2287
                     /locus_tag="ACHKAU_004919"
     CDS             1328..2287
                     /locus_tag="ACHKAU_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO5533810.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2326..3225)
                     /gene="nhaR"
                     /locus_tag="ACHKAU_004920"
     CDS             complement(2326..3225)
                     /gene="nhaR"
                     /locus_tag="ACHKAU_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO5533811.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3291..4457)
                     /gene="nhaA"
                     /locus_tag="ACHKAU_004921"
     CDS             complement(3291..4457)
                     /gene="nhaA"
                     /locus_tag="ACHKAU_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO5533812.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1434 a         1073 c          992 g         1241 t
ORIGIN      
        1 taactataat gctataactc tgggaaatgg ttggaataca ccgctgggtg ccatctcttt
       61 ttacgctgcc cgatcgagta gtaagttaaa taatgatacc agacacgaag gtacgagcta
      121 tcaggtcgcc tataataaat atctagtaca aacagccact cgatttagcg tggccgcctg
      181 gcgttatgct tcgcaggatt acaggacatt cagcgaccat ctttacgaaa atgataaaca
      241 ctatcatcag agtgattatg atgattttta tgatattggc agaaaaaata gcctttctgc
      301 taatattatg caacctttat ccaataatct gggaaatgta tcattaagtg cgctttggcg
      361 gaattactgg gggcgaagtg gaaatgctaa agattaccaa ttcagttact ccaataactg
      421 gcaacacatt agttatactt tctctgccag ccaatcttat gatgaaaata ataaagaaga
      481 ggagcgtttt aatctgttta tctccattcc tttctactgg ggggatgata ttgccaaaac
      541 acgtcaccaa attaacttat cgaattcgac ctcattttcc aaagatggct attcctccaa
      601 caatactgga attactggca tagccggtga acatgatcag ttaaattatg gtatatatgt
      661 taatcagcaa caacaaaata atgatacctc gcttggtacg aatttaagct ggagaactcc
      721 catcgccata atagatggca gctatagtca ttctaaaaac gcctggcaaa gtggtggaag
      781 tattagtagt ggattagttg tctggcccgg tggtattaat atcactaacc agttatccga
      841 tacatttgca attctggatg cgcctgggtt agaaggtgca catattaatg gacaaaaata
      901 caatcgaaca aacagcaaag gccaggttgt ttacgacctg ataatacctc atcgtgaaaa
      961 ccatctggta cttgatatag caaacagtga aagtgaaaca gaattgcagg gcaatcgtca
     1021 aattattgca ccttaccgtg gagcagtttc ttatgtgcag tttacaactg accaacgtaa
     1081 gccctggtat atacaggcac tgcgtcccga tggttcgcca ttaacctttg gctatgacgt
     1141 actggatctc caggaaaata atattggagt cgttggccag ggtagtcgcc tttttattcg
     1201 cgtagatgaa attcccactg gcataaaagt tgctctcaat gatgaacaga acttattctg
     1261 tactattact tttcaacacg ttatcgatga aaacaaaaca tatatatgcc agtaaaagga
     1321 gtttactatg aagtggcttt tattaatcac cttgagtcta tattctttta ttgtgcaaag
     1381 tgctccctgt gcattgacca atgtcggtga acaacgtgga acatatatac tcaagccact
     1441 gagtatgaaa ggtaatctaa ccgccgaaaa aattgaaaac accggaaata tcattgagtg
     1501 tacagacttg caagagacaa ccaacgccat taaagttcat tttttaacac agaacgcttt
     1561 aaggtttaaa gtcggaagtc gtcattatat tattaatttc ccctttgaat ctgacggtaa
     1621 aaaaatagag cttccaggga agtcatctta taatttagaa gaaatgctat ctgcgataaa
     1681 tttcaccatt ccatattcta tcgcatctgt atcagatagt tctaacaccg cagacatttt
     1741 tcctggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta cagggaataa
     1801 tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac aggtaaattt
     1861 aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac tgtctcgtat
     1921 caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg gcgagcaagg
     1981 tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga cgatacagga
     2041 gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg cgggtcaaat
     2101 aggctttcaa ctctccaatg atggtacgaa tgaaatccag tatggtaaaa gcaattacta
     2161 ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc gcccgcgtac
     2221 atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg tggttatgta
     2281 caactaatct atctccccca ccttaacttt ggggggaggt gccgattaac gcgccgctgg
     2341 actaaaaagc gcagaataat ccgtattgca gattcgctgt accgccgggt gctgaatcat
     2401 ccgctcagca aaaatagcat ggtactcttc catcacattc tcgacgcgac caatttctac
     2461 gacagtttta tcggcataaa agtcatatgc ataaagcgtt ggggcaacga agattgcatt
     2521 gtgcatcgca ccaaaagctt tcatcaacgc ggcatcatca aactcgccga ggatttctac
     2581 gtttaatccc tgggagttaa accagttaag caatttgcgc cctaacattg aacgtcgccc
     2641 aggaatcaaa agtcgccgtt cttccagaca agccgggaac ggtttttctg gtggtggatt
     2701 tgtacaccag aaactcacgc cacattcgcc aattctcacg gagaacaggc cttcctgctg
     2761 cgtagagtct atcggacagt cagaaatgat catatccagt ttatgctgac ttaattgctc
     2821 cagcagcatt tcgtgggtgg attcgaagca gcgaagatga atgggctcgc cttctaccac
     2881 tgcggcgtta agtacgctac tgaccaggcg tttggaaagt gcatcagcca caccaacgtc
     2941 aaacaataaa ttggattctt tgcgatagtt cacaatatcc agcatttcct ggcttaaggt
     3001 gaacatttta tcggcatagc gatagaccag ttctcccagc tcgctgggtt cgagaccacg
     3061 tcccttgcgt ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa tctgtccggt
     3121 aatggtttgt ggagttaaat aaagcgcctc cgctgcgcca accacggaac cttctttata
     3181 gacatgccag aagtaataca agtggttgta attgatatga gacatgctca tttctctccc
     3241 tgataacaat gaaaagggag ccgtttatgg ctccccggta aaccgtcctg tcaaactgat
     3301 ggacgcaaac gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat agaaccgacc
     3361 aggataccga gtttcgccca gttaatcagt tctggatcta cgctaccaaa ggccaggctg
     3421 gcaataaaga tagacatagt aaaaccgata ccgcacagga tccccaccgc cataatttgc
     3481 tgataagtcg ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa ccagcagaac
     3541 agactaatcc ccagcggttt gccaatcagc aagccagcga tgatccccaa tggcagaatg
     3601 gaggtcaagc catccagcgt gacaccttgc agtgaaacgc cagcattagc aaatgcaaac
     3661 agcggcaaaa tcagatacgc cacccacggg tgtaacacat gctccagtcg cttcgctgga
     3721 gaacgcccat gcttctcttt caaaggaata aagaagccga caattacccc cgccagggtt
     3781 gcgtgaaccc ccgatttcaa caccgcagtc cacaacacta cgccaaccag aatatagacg
     3841 cccgtgcggc gtacaccaca cagattcaat accgcgagta ccgcaattgc tacagccgcg
     3901 acgccaagag aggccatcga taagtcatta gtgtagaaca atgcgatgat aatgatggcc
     3961 ccaagatcgt cgataatagc cagagccatc aaaaagatct tcagcgctaa cggaacacga
     4021 cttcccaaca gcgccagcac accaagtgca aaggcaatgt cagtagccgc cgggatcgcc
     4081 cacccttcgc gggtaatcgg atcggcatag ttaaaggcca gatagagtaa tgccggaaca
     4141 atcatcccac caatagcggc gataactgga aatgcggcct ggcgtaagct ggctagcgat
     4201 ccttgcatca gttcacgttt aacttccaga ccgactaaca ggaaaaatac cgccatcagc
     4261 gcgtcattta tccataacag catgtttttg ttgatttcga gtgaaccaac ccggagctga
     4321 accggcgtct ccagaaagtc gtgataccat ccactggttg cgccgctatt ggccataatc
     4381 atcgccagga tagcggcaat gataagaata atgcctcccg aggcatcact gctaaagaat
     4441 cgatgcagat gtttcacttt ttatttctct ttcaggtgaa tagatcgttt ttactatttt
     4501 accccgcaca aatcatcgtt aaaatcagat tatagtgtag gaataactcg ttttttacga
     4561 tccattgaag aatttgcgcc atcagcggtg agatgagttt ttgacggctg taagcgctca
     4621 cgagtgaccc taagcccaga taatcatagc ttaaggtgag gatttctgag ggattaatta
     4681 ggaaaagtaa taattaattt cttgtcagtc ggcgttattt gttaggacaa tatttcaaaa
//
LOCUS       DAZFJF010000233         4702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFJF010000233 DAZFJF010000000
VERSION     DAZFJF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.235"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..395)
                     /locus_tag="ACHKAU_004922"
     CDS             complement(<1..395)
                     /locus_tag="ACHKAU_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5533813.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSRHDKVKQSIERLAVRGVIRNP
                     PMVVFEKINNLGLLRGVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALA"
     gene            complement(470..1204)
                     /locus_tag="ACHKAU_004923"
     CDS             complement(470..1204)
                     /locus_tag="ACHKAU_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO5533814.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLQPSLRGLQISPKARSLI
                     LWTERGAARHAKMLETDQAWDVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVW
                     LWDYANRSQALFRELYPALKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIY
                     EPDGPTNLLAWERLKNKELPPSLHRY"
     gene            complement(1479..1661)
                     /locus_tag="ACHKAU_004924"
     CDS             complement(1479..1661)
                     /locus_tag="ACHKAU_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5533815.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(1658..2185)
                     /locus_tag="ACHKAU_004925"
     CDS             complement(1658..2185)
                     /locus_tag="ACHKAU_004925"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5533816.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(2182..2628)
                     /locus_tag="ACHKAU_004926"
     CDS             complement(2182..2628)
                     /locus_tag="ACHKAU_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5533817.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            complement(2585..2821)
                     /locus_tag="ACHKAU_004927"
     CDS             complement(2585..2821)
                     /locus_tag="ACHKAU_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO5533818.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            complement(2832..3047)
                     /locus_tag="ACHKAU_004928"
     CDS             complement(2832..3047)
                     /locus_tag="ACHKAU_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533819.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            complement(3180..3458)
                     /locus_tag="ACHKAU_004929"
     CDS             complement(3180..3458)
                     /locus_tag="ACHKAU_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533820.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDFWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            complement(3529..3819)
                     /locus_tag="ACHKAU_004930"
     CDS             complement(3529..3819)
                     /locus_tag="ACHKAU_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO5533821.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVSAVTGATVTSINQAAAKMARAG
                     ILVIDGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(3816..4517)
                     /locus_tag="ACHKAU_004931"
     CDS             complement(3816..4517)
                     /locus_tag="ACHKAU_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5533822.1"
                     /translation="MKNIAAQMVNFDREQMRRITNNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(4514..>4702)
                     /locus_tag="ACHKAU_004932"
     CDS             complement(4514..>4702)
                     /locus_tag="ACHKAU_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFO5533823.1"
                     /translation="MCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTA
                     GKPKLDLTNTDWIYGVDL"
BASE COUNT         1036 a         1285 c         1075 g         1306 t
ORIGIN      
        1 gcgagagcaa gctgtttctc cagttcagcc ttctggtctt caaggtcggc cgcaaggcgc
       61 aatgcctcag aaaaggtttg tggtattttc gcggttgccc cttcgagttc tcgccagcgg
      121 tcaacaaggc gagcggtgaa ttccggcgac aactgggcaa cgacaataat gctgtcgcgc
      181 ttaccttgtt cgccctcaaa aacgtaagcc tctacgccac gaagtaatcc taagttattg
      241 attttttcga aaaccaccat tgggggattt cggatcacac ctcgaaccgc cagtcgttca
      301 atggattgtt tcaccttgtc atgacggctt cccaccaact cagcgatttc aatgcttgtc
      361 attttgatgg cattgctatt tatcagctca ttcattgtca tgtcctctca cattgaaaat
      421 tcagcaataa aaaacccagc cgaagctggg tttgttaagt tgtcaattgt cagtagcgat
      481 gcagtgaagg cggcaactct ttgttcttaa gcctttccca tgccagaagg ttcgtcggcc
      541 cgtcaggctc ataaatatct atatcccgcg tgtgattaat taaaacgccc ctcgccctcc
      601 cgatgatata cgagaactca tagccgtagt cgtggcatat gccggaatag ccagactgaa
      661 tcagttttaa tgcgggatac aactcacgga acaatgcctg tgagcggttg gcataatccc
      721 acagccatac aaggctgtct gtttcttttg cggaaagccc gttgagcttc ttctcttgtt
      781 tgccagtatt tttctcgcac tggctgaaat agcagtcttc cagtttttcg aacacatccc
      841 acgcctgatc agtttcgagc atttttgcgt gacgggctgc gcctcgttct gtccagagta
      901 tgagggagcg ggctttcggg gaaatttgta accctcttaa agatggttgc aaattttgtg
      961 agttacttaa agtaacccgc aaattttgtg agtagtttaa agctacccgc aattctttaa
     1021 ggtcattacc aacaactttg aaaaagtgtt tcccttcaac gaagcgtact ttgttctcat
     1081 gatgattctg gcgaatacgc accggctcag tgccgtaaag ctgcgccaaa agttcggtgg
     1141 taataacagg aatctggtta tgggtgatcg gggagagagt ttcaacagaa atttgagttg
     1201 tcataatgac gccctctggt ggtttcttaa taactcacca ccgacgacgc caatcgtctg
     1261 gtggtgaact gtgcagggtt ggcgtaaccg ggaaaccgac cggcgcggat ctctccgccc
     1321 ccacacagcc caccataatt cagatgtgcg cgtgcatacg acaataaaaa acacgctcgc
     1381 ggcgtgtatc tgtcgcggtc tctatccagg acgccaatcc cgacgccaga ttttgctggc
     1441 gcgtgaggaa tatagccccg aataaatcat cgcgtcaatc accttgtttt cctcgcacga
     1501 tgtcttagcc accggatatc ccacaggtga gccgtgtaat tgaaggtttt tacgtcagat
     1561 tcttttggga ttggcttgcg tttatttctg gagcgtttcg ttggaaggta tttgcagttt
     1621 tcgcagatga tgtcggtgat acttcgtcgc tgtcgcctca tgccgccctc ctgacgccct
     1681 gcccgatcgc catcaatgcc gctttggata cagtagtaaa cattcgtcga ggactgatga
     1741 acggtcgcca aatcagcagc atggagcctt tgctgtttcc cttcttctcc agccctgtcg
     1801 atggttcgat aaaattaatc cgtccatcag tgatgatgcg aacttcgtca acactctcca
     1861 gagccttgct gaaccatccg actgacatat cctctggcac aagcataact accgtctgtc
     1921 gctgttgtat gcactgctca gcggcttttt ccacccacgg cctgatattg ctgtacggtg
     1981 ggttattcca gattgcaccg tggcttaccc actcagaatt gagcgcgtcg tcggcctcag
     2041 ttagccagtg agcgcacaga gcatttttgt cgctcgctgc cgaatccagc cagaatccaa
     2101 actcaatatc cagtgcatca aaaagccaaa gcggcgtttg ccagcagtcc ttgtcgtgtg
     2161 ctggcgtatt tgatttgata gtcatgcagc cctacctttt cgttgtgacc attcatactc
     2221 tcgccgggag tcatcactcc accgcacgtt gcgctctgag ccgaaccaga acatgatttc
     2281 gataagctca gtcatgctgg ccttccgcat tttgctggta cgcacgccaa gcatgacaac
     2341 gccaccgtcg ataccaggca cacttcgttg ctccagtttt ttggtcttaa gccacagggc
     2401 agtgaacagg tctttccagt cttccggcgc cagccgttga ccatgccata gcacctgacg
     2461 cgaaacatcg ttcagcatcg gccacatacg gtcattctgc gctttgctgc gcttgggttc
     2521 tttaacgtgg acttcgtggg gtgacttgtc gtcgatgggt agtgagagaa tggcgtctat
     2581 ggcgttattt ctgattgctt cgttgcgaag cagaaaggtt tgcttcatct cctgctctcc
     2641 ggttccattt ttcagccgcc gcagcaactg atggtgccca tgcccccctg gcttcacaga
     2701 ggtcacattc tgcatagccc cacacatcaa tatttattcc ggcctcaacc cacagacgag
     2761 cattaccgcc gcaaaacgga cattctttta gctttggctg ggttaatgat aggtcgctca
     2821 tgctcactcc ttcactttaa atccagactc cggataattc tgttgcgctg aaactcattg
     2881 ttgagtttga acaaccgtcg aagaacacgg tcacgcggat agcgtcgtgc ggcaggtgaa
     2941 tgctcataca actcatcaag cggcaaactg gacgatgaac gataccgata ccaacgcacc
     3001 aactcttcac gaaaattagc cctgacaagc tcagctatcg tactcatttc ttaaaacctc
     3061 ctcaaacgca ttctgacgca tttttcattc tcgctgctta ttggcatgcc ttgcacgcgt
     3121 ttacctcgct acagagcgat tgtgatgcct taaaagcgat ttattgaagt gatatttgct
     3181 taatcgaaat tcttttcttt gattcctgcg gccctgatgg ctttcattac tgcaattacc
     3241 gttttgtcac gcccatcctc ataacccatc gcataagcac cttcttcacc atctttccaa
     3301 aagtcgtcat tcgattcggg ccagtcgata tccagttcaa tagcagagcg cgatgcctgc
     3361 catatcaccc aggcaaactc ttttaattca tcgtctcccg tgaactggct tttgtctttt
     3421 gaccaccagt tttcaaactg tcggtagcta tcgttcactt ccctctcccc caaataaaaa
     3481 ggcctgcgat taccagcagg cctgttatta gctcagtgat gtagatggtc atcttttaac
     3541 tccatatacc gccaataccc gtttcatcgc tgcactctgg cgacactcct taaaaatcag
     3601 gttcgtgctc atctttcctt cccgttcttc cctggtagca aaccggtaat acaccgttcg
     3661 ccagacctta ccatcaatga ccaggattcc tgcccgcgcc attttagccg cagcctgatt
     3721 tatgctggtt acggttgcgc ctgttaccgc ggaaacgtcc tgtgcacaga agttcttatg
     3781 cgtcccgagg taatgaataa ttgcctcttt gcccgtcata cacttgctcc tttcagtccg
     3841 aacttagctt taatttctgc gatcttcgcc agagcctgtg ctcgatttag aggtctgccg
     3901 cccatgacag gaagttgttt tactggttca ggtatcgtct caccacggtt aattcgtgct
     3961 gtcatacagg tcagttcatc ggcagccttg cgccgtaatt ccgcgtcagt cagcgcattg
     4021 gcccgcatgt tctggtacag gttggtaacc agccagtagt gcgcgtttga tttccacgga
     4081 taagactctg catccggata caggccacgc ttccggcaat actcgtaaac catatcaacc
     4141 agctcgctga cgtttggcag tccggcgata acggatgctt cttcccggca ccatgcaaca
     4201 aactgcccgg gtgatggaag aaatggtcga ttctgccgac gggctacgcg cattcctgcg
     4261 ttaacctgtt ccatcgaggt gatcccgttt tcccggaaag ccagaaccca ctggcggcgg
     4321 atttcattca gttcgttctg gtcccggtta gccagactcg ccgggaaagt tgccagtaac
     4381 tggctgaaca caccgttgat gatctgcgct acctgttgta cctgcggctt ttcgtcgtac
     4441 tgttccggca tgttgttggt gatccgacgc atctgctcac ggtcaaagtt aaccatctgt
     4501 gcggcgatgt ttttcataaa tccaccccgt aaatccagtc agtgtttgtc aggtcgagtt
     4561 ttggttttcc agctgtcacg ccagcctgtt gcttgttacg gttgatttcg agttgggtcc
     4621 acttgtcgcg gagtttggcc ggacttagca cgttaccgga ccagaagttg tcctggcatg
     4681 cccagcggaa cagcacgcac at
//
LOCUS       DAZFJF010000234         4613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFJF010000234 DAZFJF010000000
VERSION     DAZFJF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.236"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1208)
                     /locus_tag="ACHKAU_004933"
     CDS             complement(<1..1208)
                     /locus_tag="ACHKAU_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO5533824.1"
                     /translation="MIHPGSFLDKTINNTRGKNVNANIKHSQIQEIKRNFTYKKNDDI
                     SSRFKFYSSLVKQKNSTEDVVLIGKMISDEVRNYSTIHNDRNIIINSGNWKTSFLCNL
                     ARLLYSMFNGSVYFPSRDDENSSSPSPTLPTIHQSERKELSQQKSINHLKKSDTIDEY
                     IKIRKTRGIEDQITTTSQSLIINELLNGVNRNTIPFQKISELNDIIHSYENMQTKNSR
                     KGIEILVKQGELLSSLINYDKENKQLSDNISKIINLLGIEYQSHKVDIEPFIHAVWVA
                     GAPPENTSSYITAFLNTYKNYTYLLWIDPNAFGAAKFSGILKNIAMNYAIMRLRRTNP
                     HLAEKMNDVILKIQHIQNETVDFNETRERVKELENKYKSLTRETKEKFNVFFLESMIG
                     MQDNYFTYCIS"
     gene            complement(1323..1553)
                     /locus_tag="ACHKAU_004934"
     CDS             complement(1323..1553)
                     /locus_tag="ACHKAU_004934"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO5533825.1"
                     /translation="MNCWRCFPLFFIGILTTDEWGSHIRYVIEGDSSIFIQHIECMRI
                     LIKHLTRRAICFPHSVNFIKKHRYFIEKYMFY"
     gene            1476..1968
                     /locus_tag="ACHKAU_004935"
                     /pseudo
     CDS             1476..1968
                     /locus_tag="ACHKAU_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2035..2163)
                     /locus_tag="ACHKAU_004936"
     CDS             complement(2035..2163)
                     /locus_tag="ACHKAU_004936"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO5533826.1"
                     /translation="MFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPE"
     gene            complement(2194..2960)
                     /locus_tag="ACHKAU_004937"
                     /pseudo
     CDS             complement(2194..2960)
                     /locus_tag="ACHKAU_004937"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2996..3535)
                     /locus_tag="ACHKAU_004938"
     CDS             complement(2996..3535)
                     /locus_tag="ACHKAU_004938"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO5533827.1"
                     /translation="MLTTSESQFRQKDQQLSLVEETEAFLCSALARAEEKIEEDEREI
                     EHLQTQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDAPQVPRQLRQ
                     PRHRRPLPAHLPHAIHRLESEESCCPECGGELDYLEEVSAEQLELVSSALKVIRTVRV
                     KKPVQNVTASLKHRRRPAR"
     gene            complement(3547..4613)
                     /locus_tag="ACHKAU_004939"
                     /pseudo
     CDS             complement(3547..4613)
                     /locus_tag="ACHKAU_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT         1088 a         1094 c         1014 g         1417 t
ORIGIN      
        1 gatatacaat atgtgaaata gttgtcttgc atacctatca ttgactcaag aaaaaaaaca
       61 ttgaattttt ctttggtttc tctcgttaat gatttgtatt tattttcaag ttcctttacc
      121 ctctctcttg tttcattaaa atctactgtt tcattttgta tgtgttgtat ttttaatatc
      181 acatcattca ttttttccgc taaatgagga ttggttcttc tcaaacgcat aattgcataa
      241 ttcatggcta tattttttaa aattccacta aacttggctg caccaaaagc attgggatca
      301 atccagagca agtaagtata atttttatat gtatttaaaa aagctgttat atatgacgat
      361 gtattttcag gaggtgcacc tgcaacccat actgcatgta taaatggctc tatgtctact
      421 ttatgtgact gatactctat ccctaataaa tttattattt tagatatatt gtctgataat
      481 tgtttgtttt ctttgtcata atttattaat gatgataaca gttctccctg cttaactaat
      541 atttctatac cttttcggct atttttagtt tgcatatttt catatgaatg tatgatgtca
      601 ttgagctcac ttattttctg aaatggaatg gtatttctat taactccatt taacagctca
      661 ttaattatca aactttgaga ggtagttgtt atttgatctt caatgcctct tgtttttctt
      721 attttaatgt attcgtcgat ggtatcactt ttttttaaat gattgatgct tttttgttgt
      781 gataactcct tcctttcaga ctgatgtata gtaggtagag ttgggctggg ggatgagcta
      841 ttttcatcat cacgggaagg aaaatagacg ctaccattaa acatgctata tagtagtctg
      901 gcaagattac ataaaaaaga tgttttccaa tttccagagt taattatgat atttctatca
      961 ttatgtatgg tgctgtaatt tctaacttca tctgaaatca tttttcctat caaaacaaca
     1021 tcttctgttg agtttttctg ctttactagc gagctgtaaa atttaaatct ggatgagatg
     1081 tcatcatttt ttttataggt gaaattcctt tttatctctt gaatttgact atgtttgata
     1141 ttagcattaa catttttacc tcttgtattg ttaattgtct tatctaaaaa agaacctgga
     1201 tgaatcatat gtatactctc tagttacgca acaattaatg aattttgatt ggtgacaacc
     1261 tcaaatgtat tttgaggttg ttatataaaa cacattattt tatatgattt ggtgatatcc
     1321 aattagtaga acatgtattt ttcgataaag taccggtgtt tttttatgaa atttacagag
     1381 tgcgggaaac agatagccct acgggtcaga tgtttgatta atattctcat gcactcaata
     1441 tgctgaataa aaatagatga gtctccttct ataacatatc tgatgtgact accccattcg
     1501 tcagtggtca atataccaat gaaaaaaaga gggaagcagc gccagcaatt cacaacagta
     1561 taggtattgc tgctgcagcc cgtgagctca gcctgtatga atcacagtct acaactgggt
     1621 agtcaacaac aaaatcagta gacgttcttc tgaacgtgag ctggagatgt ctaccgatgt
     1681 tgcacgtctc aaacgccagc tggcggaacg ggatgaagag ctgaccatcc tccaaaaggc
     1741 cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     1801 gtgttcaaca tcaaggcaat gtgccatgtg ctccaggaag gccgcagtga ctggtatatg
     1861 tggtgtcagc ggcggacaag gataagtaca tgtcagcagt tccgtcagca ctgcgacagc
     1921 gtggtactcg cggcttttat ccggtcaaaa cagcgctacg gagcaccgta ttgacgctta
     1981 tttattggtg agtactacgt tccatggcag gagttcgtca acacggttgg agggctattc
     2041 cggtagtacg ctcagaatat ggcgcagata cgcttccgga tcgataccgt tcagacggca
     2101 ggtgccgatc agcccgtaca gcagtgctcc acgctcgccg ccgtgatcgc taccgaagaa
     2161 catgtaattc tttttcccga gacagacggc acgaagcgct ctttccgctg tgttattgtc
     2221 cgcctccgcc agaccgtcat cactgtaata acagagggcg tcccactgat tcagtacata
     2281 gctgaacgct tcgcctggtc tggatttttt cgacagcgtg ccattcttct ccaccatcca
     2341 ttcatgcagc gacgtcagta acgctttgct tcgctgctgc ctgacttcaa gacgctctga
     2401 ctctggtaat ccccgtattt catcctcgat ggcgtacagt tcactgatgc gcttcagggc
     2461 ttcttctgcc gtcgcacttt tgctgctgat gtatacatcg tggatttttc tccggacatg
     2521 ggctcagcac gcaacttctg tcagcgcacc atcttcacgt tctgcactga acagccgatc
     2581 gtaaccgttg aacgcatccg cctgcagggg gcggaggtgt tgctccgggt gtttcccctg
     2641 ccggttcggt gagtacgcga accagaccgc cggaggagat gacgaacccg cattgcgatc
     2701 attccggaca tacgtccaga tgcgccctgt tttcgtcttt tcaggcccag tgccagtacc
     2761 tttaccggtg tgtcgtcagt gtgaagcttg cgggtattca tcacataacg gtacagggca
     2821 tcattcagcg gagtcattaa ctggcagcac gcgtcaaccc agttggagag taatgcacgg
     2881 ctcagttcga caccctgtcg ggcaaagatt tcactctgac gatacagtgg cagctgttcg
     2941 cagtattttc ccgttaacac gcgggcaagt aatccggggc ccgcgatacc acgctctatc
     3001 gggcgggacg gcgccggtgc ttcaacgatg cagtcacatt ttgtacaggc ttttttaccc
     3061 gaacagtgcg gatcactttc agggcgctgc tcaccagttc cagttgttct gcactgactt
     3121 cctccagata atccagctca ccgccacact ccgggcaaca gctttcttca gactccagac
     3181 ggtgtattgc atggggaagg tgtgccggta acggacgacg atggcgcggc tgtctcaact
     3241 ggcggggaac ctgtggagcg tcttcccgcc cactgtaacg atcgctgtcc tgttcgcgtt
     3301 gtttcagcag ggcctcagcc tgttcaactt cacgacgcag tttttcagaa cgggtaccga
     3361 acagcatccg gcgcagtttt tctatctgag tctgcagatg ttctatttcc cgttcatctt
     3421 cttcgatctt ttcttcggca cgtgccagtg cagagcacag gaaggcctcc gtctcttcaa
     3481 ccagacttag ttgctggtct ttctgacgga actggctttc tgaagtggtc aacaaaaact
     3541 ggccacctag ttagagtttt tccagtatcg attttccgat tcgtttgggg gtaacccacc
     3601 gttatattcg tgcggtctta gtgcgctgta atatccaacg atatagtccg ttatggcgtg
     3661 agctgcctcg ctgaagctta cgtaacccac caccggcatc cattcgttct cttcagactc
     3721 ctgaagaagc gttccattgg gctgttatcc cagcagtttc cgcgccggct catactctgt
     3781 ctgatctggt atcgccacaa taactgccgg aactgcctgc tcgtataatg actgccctga
     3841 tcgctgtgga acatcacccc gccgggctta ccacgggttt cccatgccat ttcccgcgct
     3901 ttcatggtga gcctgctgtc cggcgagaac gacatggccc agcccactgg ttttcttgcg
     3961 aacaggtcga gaacaacggc gaggtacgcc cagcgcttac ccgtccagat ataggtcaca
     4021 tcaccgcacc acacctgatt tggctcggtc acggcgaact gcctttcaag gtagttaggg
     4081 atagcaacat gttcatgacc accacgttta taccggtgag tcggctgctg acagctgacc
     4141 agccccagct ctttcatgag cctgccagca agccagcgtc ccatctggta gcctctccgg
     4201 gttgccattg tggcgatgct tcttgctccg gccgaaccgt ggctgatgcc atgtagctca
     4261 agtacctgac tgcgtaatac agcccgtctg ccgtctggtt tttcaggacg gtttttccag
     4321 tatctgtagc tgctgcgatg aaccccgaac acatggcaga gtgtgaccac aggataatgc
     4381 gctctgagtt tcccgattat cgagaactgt tcagggagtc tgacatcaag agcgcggtag
     4441 ccttttttaa tatttcattc tccatttcaa tgcgttgtag ctttttcctc agcttacgta
     4501 tttcgatttg ttctggtgtt atcggagagg cttttggtgt tttgccctga cgctcatcac
     4561 gcagttgttt gacccatctt gtcattgtag aaaggccaac atccatagct ttg
//
LOCUS       DAZFJF010000235         4612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFJF010000235 DAZFJF010000000
VERSION     DAZFJF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.237"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..215
                     /locus_tag="ACHKAU_004940"
     CDS             <1..215
                     /locus_tag="ACHKAU_004940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533828.1"
                     /translation="KKPHLRFFFLRHAVGGVFACGSPQEGCFFACGTPQGGCFFACGT
                     PQVCTNIIAFFLSSANVLYGDEVSPT"
     gene            complement(1205..1396)
                     /locus_tag="ACHKAU_004941"
     CDS             complement(1205..1396)
                     /locus_tag="ACHKAU_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO5533829.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1461..1784
                     /locus_tag="ACHKAU_004942"
     CDS             1461..1784
                     /locus_tag="ACHKAU_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO5533830.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1993..2424
                     /locus_tag="ACHKAU_004943"
                     /pseudo
     CDS             1993..2424
                     /locus_tag="ACHKAU_004943"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2462..2980
                     /locus_tag="ACHKAU_004944"
     CDS             2462..2980
                     /locus_tag="ACHKAU_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO5533831.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSN
                     ETKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTV
                     SGGTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKG"
     gene            2987..3220
                     /locus_tag="ACHKAU_004945"
     CDS             2987..3220
                     /locus_tag="ACHKAU_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO5533832.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            3584..3820
                     /locus_tag="ACHKAU_004946"
     CDS             3584..3820
                     /locus_tag="ACHKAU_004946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533833.1"
                     /translation="MSIIIDREKYTALMTLIQTSQGYVGSKVQPNGYTLKCSFDTGFA
                     CCISRNTRNSNLSVHFENESTDPATHFAILSAIS"
     gene            complement(3941..>4612)
                     /locus_tag="ACHKAU_004947"
     CDS             complement(3941..>4612)
                     /locus_tag="ACHKAU_004947"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFO5533834.1"
                     /translation="ISKMELTPLKTIVYVDGFNFYYRKLKDSNKKWLNIKAMVEKLLP
                     DDEIIGINYYTARISGRIDSDAPRRQQIYFNALKSIDNLSIHYGTFSVNESRMFLVQP
                     LDFKHHLKNPNQKIKFANVLKTEEKGSDVNLGCHLVRDGFQGKYEKAVIITNDTDLAE
                     PMRVVKSELSLYIELLSPVNKPARTLSAICDKVTLISDQSIADSQFEDVIVVGEKTVT
                     KPAQW"
BASE COUNT         1132 a         1020 c         1284 g         1176 t
ORIGIN      
        1 aaaaaaaacc gcatctgcgg ttttttttcc tacggcacgc cgtaggaggg gtgtttgcct
       61 gcggctcgcc acaggagggt tgtttcttcg cctgcggcac gccacagggg ggttgtttct
      121 tcgcctgcgg cacgccacag gtctgtacca acattattgc attttttttg tctagtgcaa
      181 atgttttata tggtgacgaa gtaagcccta cctaaaggag aaaagatctt cttgagatcc
      241 tttttttctg cgcgtaatct cctgctttga aaacgaaaaa accaccctgg caggtggttt
      301 ttcgaaggtt agttaatcct ggcagattct ctaaccgcgg taacggtctt gtgcgagaca
      361 tgtcaccaaa tctgtccttt cagtgtagcc gtagtcaggc cgccacttca agaactctcg
      421 atacatctct cgcacatcct gtttaccagt ggccgttgcc aatggcgatt agtcgtgtct
      481 ttccgggttg gactcaagtt gatagttacc ggataagacg cagcggtcgg actgaacggg
      541 gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggagccga gtgtcaggcg
      601 tggaatgaga aaccgcggcc ataacagcgg aatgacaccg gtacaccgaa aggcaggaac
      661 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      721 gtttcgccac ctctgacttg agcgtcaatt tttgtgatgc tcgtcagggg ggcggagcct
      781 atggaaaaac gcctgcggcg cggccttttt ccctggtttt gcctttttgt tcacatgttc
      841 tttccggcct tatcccctga ttctgtggat aaccgcatta ccgtctttgc gtgagctgat
      901 accgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaagag
      961 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     1021 tcttttctta taatctgcac tgatgccaca tagttaagcc agtatacact ccgctatcgc
     1081 tacgtgactg ggtcatggct gcgccccgac acccgctaaa accccctgac gcgccctgac
     1141 gggcttgtct gctcccggca tccgctcaca gacaagctgt gaccgtctcc gggagctgta
     1201 tgtgtcagag gttttcaccg tcatccccga agcgtgcgag gcagctgcgg taaagctcgt
     1261 cggcgtggtc gtgaagtgat tcacaaatat cggcctgttc atctgcgtcc agttcgttga
     1321 gcttctccag cagcgttaat gtctggcttc ttataaagcg ggccatgtta agggcggttt
     1381 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     1441 taccgatgaa acgcgagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     1501 tacttgaacg ctgtgagggt aaacaactgg cggtatggat gcggcgggtc tgcctggggg
     1561 agccggttgc ccgttccggg aaactgccga cactggcacc gccgttactg cgtcagctgg
     1621 ccgccatcgg gaataacctg aatcagacag cgaggaaggt gaacagcggg cagtggtctt
     1681 ccggtgaccg ggttcaggtg gtggccgcac tgatggccat cggggatgag ctgcgccggc
     1741 tgcgtctggc cgtcagggaa caggggacgc gggatgatag ttaagtttca tgccagggga
     1801 aaaggcggtg gcagtggtcc ggttgattac ctgctgggga gggagcgtaa ccgggagggc
     1861 gcaaaggtgc ttcagggtaa cccggaagaa gtccgggaac tcatcgatgc cacgccattt
     1921 gcgaagaaat acacgtccgg tgttctgtcg ttcgcggaga aggaactgcc gccgggagga
     1981 cgtgaaaaag tgatggcgag ttttgagcgt gtactgatgc ccggtctcga aaaaaatcag
     2041 tacagcattc tgtgggtgga gcaccaggac aaggggcggc ttgagcttaa ttttgtcatt
     2101 ccgaacatgg agctacagag cggaaaacgc ctccagccgt actacgaccg cgcagacagg
     2161 ccgagaattg atgcctggca gacgctggtg aatcaccatt acgggctgca tgaccccaac
     2221 gccccggaga accgccggat actgactctc cctgataacc tgccggaaac gaaacaggcg
     2281 cttgcagaga gcgtcacacg cgggatagac gctctttatc atgtcgggga gataaaaggc
     2341 cgtcaggatg tgattcaggc gctcacagag gcagggcttg aagtggtcag ggtgacgcga
     2401 agcagtatca gcattgcaga cccgaacggt gggaagaata tcaggctgaa aggagcattt
     2461 tatgagcaat cttttgcaga cgggcgcgga gttcgagaaa aagctgaaag agagagccga
     2521 atctacagag aaaatgctga acaacgagtt caggaggctc ggcgaatctg taagcgaggc
     2581 tgtgacatca aacgagacga aaatcagaga cgctatagcc ctgttcacag cctcgacaga
     2641 ggaatctctg gaaaaacacc gggaaggggt gaaagaggcg atgatgcagc acaggaggga
     2701 cgtgttaaag ctggcaggga atacgggcat gatgttgctg gggatagtct ttctcctgtt
     2761 taccgtgagt ggcgggacgc tctggtatct tggcgggagg atacaggcga acctggaaga
     2821 tatcaggaag caggaagaga cattgcagaa actgaacgcg aagacatggg gcgtggagtt
     2881 tgtgcaggac gggaacagga aatttctggt ccttccttac gggaagtcag cagaggtgat
     2941 ttcctttcag gggaaggaat gggtacatct gaaggggtga cacagagtga cagagctgga
     3001 aacacatttg ctgaacgcct tagagcagct gcaacaggac tatatgcagc ggctgagcga
     3061 atgggagagc gccttcgtgg aattgcagaa gatgttttcg cttacgcaac gggacaacgc
     3121 gatgctgaac gagcgggtca tgcagttgag tcagcaggtg cagcacttga gcgagcagac
     3181 agaacgcttg agccagttat acagcgagaa ctggagataa gagaggaacg gttgatacag
     3241 gagcgcgaat atgcgttatc cctggaacgg gagcgtcagc cggaaataca ggaacgcacg
     3301 ctggatggcc cttcgctggg atggtgaaac tatgaaaaat ggcaggttca gtggattttg
     3361 acggactaat gtagtctgca ccatcttgtt gcataggtat tcatgcggtt aaaatttatc
     3421 aggcgcgatc gcggcagttt ttcgggaggt ttgttgcctg tttttccggt ttcccgtcag
     3481 aaacgcgctg agagcgtctg aggcggtgcg ggcaacggga gttatggtaa atagatttat
     3541 tgctgaggtt taatgcctgc agagtaatac taatggggat gtgatgagta ttattattga
     3601 tcgtgaaaaa tatactgctt tgatgacgtt gatccagaca tctcaaggat atgttggctc
     3661 aaaagtacaa ccaaatggat atacattgaa gtgttctttc gacactggtt ttgcatgttg
     3721 tatctctaga aatactagaa atagtaactt atctgttcat tttgaaaatg aaagtacaga
     3781 tcctgcaact cactttgcta ttttaagtgc tattagctaa ttttttattt tctgcacata
     3841 gctatagatt tcatactctt tatatttgcc tgaaggtata atgcaaaaca taaaaagccc
     3901 gggggacggg ctttttttca acagaacgag tatgtagcca ctaccactgt gctggttttg
     3961 tcacggtttt ctcaccaact acaataacgt cttcaaactg gctatcagct attgattggt
     4021 cgcttataag agtgacctta tcgcatattg cgctcagggt tcttgctggt ttgtttacag
     4081 gagaaagtag ttcaatataa aggctgagct ctgatttgac tactctcatt ggttcagcca
     4141 aatctgtatc attagttatt attacagcct tctcgtattt cccttggaaa ccatctctaa
     4201 ccaagtggca acctaaatta acgtcagaac ccttttcttc tgtctttaag acgtttgcaa
     4261 actttatttt ttgattcggg ttttttaagt gatgtttgaa atcaagaggc tgcactaaaa
     4321 acattctgct ttcattcacc gaaaaagtac cataatggat acttaggtta tctatgcttt
     4381 tcagtgcatt gaaatatatt tgttgcctac gaggtgcgtc agaatcaatc cttcctgaga
     4441 tcctagccgt gtaatagtta attcctatta tttcatcatc cggtagcaat ttttcgacca
     4501 ttgcttttat gttcagccac tttttgttgg aatctttcag cttacgataa taaaagttaa
     4561 agccatcaac ataaactatt gttttcaatg gggttagctc cattttagat at
//
LOCUS       DAZFJF010000236         4611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFJF010000236 DAZFJF010000000
VERSION     DAZFJF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.238"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..165
                     /gene="kil"
                     /locus_tag="ACHKAU_004948"
     CDS             <1..165
                     /gene="kil"
                     /locus_tag="ACHKAU_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5533835.1"
                     /translation="DYVLHEGRTYIASANNIKKRKLYIRNLTTKTCISDCMIKVFLGR
                     DGLPVKAESW"
     gene            165..335
                     /locus_tag="ACHKAU_004949"
     CDS             165..335
                     /locus_tag="ACHKAU_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO5533836.1"
                     /translation="MTKKIKCAYHLCNKGIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     NCAEKDQMAHEL"
     gene            409..684
                     /locus_tag="ACHKAU_004950"
     CDS             409..684
                     /locus_tag="ACHKAU_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533837.1"
                     /translation="MITNYEVTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            783..3434
                     /gene="recE"
                     /locus_tag="ACHKAU_004951"
     CDS             783..3434
                     /gene="recE"
                     /locus_tag="ACHKAU_004951"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO5533838.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQN
                     QPETEKISPDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQ
                     VEAKEKDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNEN
                     YHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRF
                     IVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMALPLGQWLVE
                     SAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQ
                     DAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTL
                     ADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            3427..4236
                     /gene="recT"
                     /locus_tag="ACHKAU_004952"
     CDS             3427..4236
                     /gene="recT"
                     /locus_tag="ACHKAU_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO5533839.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4293..4487
                     /gene="ralR"
                     /locus_tag="ACHKAU_004953"
     CDS             4293..4487
                     /gene="ralR"
                     /locus_tag="ACHKAU_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO5533840.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            4480..>4611
                     /gene="rcbA"
                     /locus_tag="ACHKAU_004954"
     CDS             4480..>4611
                     /gene="rcbA"
                     /locus_tag="ACHKAU_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HFO5533841.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     "
BASE COUNT         1438 a         1047 c         1109 g         1017 t
ORIGIN      
        1 gattatgttc ttcatgaagg ccggacatat atcgcctcgg caaacaatat taaaaagcga
       61 aaactttata ttcgtaacct gactacaaaa acatgcattt ctgactgcat gattaaagtc
      121 ttcctcggtc gtgatggttt acctgtaaag gcggagtcat ggtgatgact aagaaaataa
      181 aatgtgctta ccacctttgc aataaaggaa ttgaagaaag caaaagcatt aaaagaccac
      241 ttcatttcat gcgtggagtt atcccaacga cggaaatgaa aaaatattgt agtgaaaatt
      301 gtgccgaaaa agaccagatg gcacacgaac tttaattaac tgactatgcg aaactgaatt
      361 tatgccagca atggcaggga ttcgctcaac cttaattaag gagaaaaaat gattaccaat
      421 tatgaagtca ctgttgtaac taccgatgac attgttcacg aggttaatct ggaaggaaag
      481 cgtattggct acgtgattaa aacagaaaat aaagaaaccc cattcactgt ggttgatatt
      541 gacggtccat caggcaacgt aaaaacactt gatgaaggtg tcacaaaaat gagtctggtt
      601 cacatcggaa agaatctgcc cgcagaaaaa aaagccggat ttctggcaac tctgattgca
      661 atgaaattaa aaggtgaaat ctgaaaaaag aaagcctgca caacgtgcag gcctgagtga
      721 agaacctggg acatttattc atcactcgca gtaattttaa tctgagttga ggttaaaaaa
      781 caatgagcac aaaaccactc ttccttttac ggaaagcgaa aaaatcatcc ggtgaacctg
      841 acgtcgtcct gtgggcaagc gacgattttg aatcaacctg tgccactctg gactacctga
      901 tcgttaagtc aggtaaaaaa ctgagcaact attttaaagc tgttgccaca aattttcctg
      961 tcgttaatga ccttccccct gaaggtgaga tcgattttac ctggagtgaa cgctatcaac
     1021 tcagcaaaga ctccatgacc tgggaactaa aaccgggagc agcgccagac gacgttcacc
     1081 atcaggagga tgcaccggaa accgaagaac cgacgggagg ccaggaagaa aacgcgcagg
     1141 cagacgccca cgaggattgc caggattgcg aagtctctgt agccactttg cggttcacac
     1201 agcgtcttct gcacattttt acgtatgcag ccggggatcg aaaatacctg catcatgcca
     1261 cccgtgaaca acgcaaacac attactgctc ttgagatgga tcaggaaaac agctatgtcc
     1321 agaatctgct gttggccata cgcagcatgg cagaaccgac aactctggat aatgccgccc
     1381 tgctccgcct gactgatgca attaaggcag tgttctctat cacgaaaaaa catcagccct
     1441 atgaatttaa gaatttcatt tcagcctggc tggataccga acacattgat cgcggtcttc
     1501 tgacaaaaga atggcgaaaa gggaatcgtg tttcacgcat cactcgcacg gcttccggtg
     1561 ctaatgctgg cggcgggaac ctcaccgatc gcggcgaagg tttcgtccac gatctgacat
     1621 cactggcgcg cgatgtagcc actggcgtac tggcccgttc aatggacgtg gacatctata
     1681 accttcatcc ggcacacgct aaacgcattg aggaaattat cgctgaaaat aaaccaccct
     1741 tttctgtttt ccgcgacaaa ttcatcacca tgcctggcgg gctggattat tcccgcgcca
     1801 tcgtggttgc gtccgtgaaa gaagcaccaa ttgggatcga ggtcatcccc gcacacgtca
     1861 ctgaatatct gaacaaagta ctgactgaaa ccgatcatgc caaccctgat ccggaaatcg
     1921 tggatattgc ctgcggtcgc tcctcggccc cgatgccgca acgagtaaca gaagaaggaa
     1981 aacaggacga tgaagaaaaa ccacaaccat ctggcgcaat ggcagatgaa caggcaacgg
     2041 ctgaaacagt ggaaccgaat gcaactgaac atcatcagaa cacgcagccg ctggatgctc
     2101 agtcacaggt aaattctgtt gatgcgaaat atcaggaact gcgggcagaa ctccatgaag
     2161 cccggaaaaa cattccatca aaaaatcctg tcgatgccga taaattgctt gctgcatcac
     2221 gtggtgaatt tgttgacgga attagcgacc cgaacgatcc gaaatgggtt aaggggatcc
     2281 agactcgcga ttctgtgtac cagaaccagc cagaaacgga aaaaatcagc ccggatgcga
     2341 aacaacctga gccagtagtg caacaggaac cggaaatagt ctgcaatgcc tgcggtcaga
     2401 ctggcgggga taactgccct gactgtggtg cggtgatggg cgacgcaaca taccaggaaa
     2461 cattcggtga agagaatcag gttgaagcta aggaaaaaga tccggaggaa atggaaggcg
     2521 ctgaacatcc gcacaatgag aatgctggca gcgatccgca tcgcgattgc agtgatgaaa
     2581 ctggcgaagt cgcagatccc gtaatcgtag aagacataga gccaggtatt tattacggaa
     2641 tttcgaatga gaattaccac gcgggtcccg gcgtcagtaa gtctcagctc gatgacattg
     2701 ctgatactcc ggcactgtat ttgtggcgta aaaatgcccc cgtggacact acaaagacaa
     2761 aaacgctcga tttaggaacc gctttccact gccgggtact tgaaccggaa gaattcagta
     2821 accgctttat cgtagcacct gaatttaacc gccgtacaaa cgccggaaaa gaagaagaga
     2881 aagcgtttct gatggaatgc gcaagcacag gaaaaacggt tatcactgcg gaagaaggcc
     2941 ggaaaattga actcatgtat caacgcgtta tggctttgcc gctggggcaa tggcttgttg
     3001 aaagcgccgg acacgctgaa tcatcaattt actgggaaga tccggaaaca ggaattttgt
     3061 gtcggtgccg tccggacaaa attattcctg aatttcactg gatcatggac gtgaaaacca
     3121 cagcggatat tcaacgattc aaaacggctt attacgacta ccgctatcac gttcaggatg
     3181 cattctacag tgacggttat gaagcacagt ttggcgtgct gccaactttc gtttttctgg
     3241 ttgccagcac aactgttgaa tgcggacgtt acccggttga gattttcatg atgggcgaag
     3301 aagcaaaact ggcaggccag caggaatatc accgcaatct gaggaccctg gctgactgcc
     3361 tgaataccga tgaatggcca gctattaaaa cgttatcact gccccgctgg gctaaggagt
     3421 atgcaaatga ctaagcaacc acctatcgca aaagccgatc tgcaaaaaac ccagggaaac
     3481 cgtgcaccag cagcagtaaa aaataacgac gtgatcagct ttattaatca gccatcaatg
     3541 aaagagcaac tggcagcagc tctcccacgc catatgacgg ctgaacgaat gatacgtatc
     3601 gccaccacag aaattcgtaa ggttccggcg ctaggaaact gtgacaccat gagttttgtc
     3661 agtgcgatcg ttcagtgttc acagctcggc cttgagccag gtagcgccct cggccacgca
     3721 tatttactgc cttttggtaa taaaaacgaa aagagcggta aaaagaacgt tcagctaatc
     3781 attggttatc gcggcatgat tgatctggct cgccgttctg gtcaaatcgc cagcctgtca
     3841 gcccgtgttg tccgtgaagg tgacgagttt agttttgaat ttggccttga tgaaaagtta
     3901 atacaccgcc cgggagaaaa cgaagatgca ccagtgaccc acgtctatgc tgtcgcaaga
     3961 ctgaaagacg gagggactca gtttgaagtt atgacgcgca aacagattga actggtgcgc
     4021 agccagagta aggctggtaa taacgggcca tgggtaactc actgggaaga aatggcaaaa
     4081 aaaacggcta ttcgtcgcct gtttaaatac ctgcctgtct caattgaaat ccagcgtgca
     4141 gtatcaatgg atgaaaagga accactgaca atcgatccgg cagactcctc tgtattaacc
     4201 ggggaataca gtgtaatcga taattcagaa gaataattca gcctggcggt gtaatgcacc
     4261 gccaacgtga gacagttttt atgacaaaaa ttatgagata tgacgatgtt aaaccatgtc
     4321 cgttttgtgg ttgtccatct gttacagtga aagcaatttc aggatattac cgggcaaaat
     4381 gcaacggatg cgaatcccga actggctatg gtggaagtga aaaagaagca ctcgaaagat
     4441 ggaataaacg aactactgga aataataatg gaggtgttca tgtataaaat aactgccact
     4501 attgaaaaag aaggtggcac tcctactaac tggacaagat attcaaaatc taaattaacg
     4561 aaatcagaat gcgaaaaaat gctctcaggg aaaaaagaag caggcgtgtc c
//
LOCUS       DAZFJF010000237         4527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFJF010000237 DAZFJF010000000
VERSION     DAZFJF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.239"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..85)
                     /locus_tag="ACHKAU_004955"
     CDS             complement(<1..85)
                     /locus_tag="ACHKAU_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5533842.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADA"
     gene            complement(66..542)
                     /locus_tag="ACHKAU_004956"
     CDS             complement(66..542)
                     /locus_tag="ACHKAU_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5533843.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            629..743
                     /locus_tag="ACHKAU_004957"
                     /pseudo
     CDS             629..743
                     /locus_tag="ACHKAU_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(794..2304)
                     /locus_tag="ACHKAU_004958"
                     /pseudo
     CDS             complement(794..2304)
                     /locus_tag="ACHKAU_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2924..3931)
                     /locus_tag="ACHKAU_004959"
                     /pseudo
     CDS             complement(2924..3931)
                     /locus_tag="ACHKAU_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1098 a         1186 c         1037 g         1206 t
ORIGIN      
        1 cggcatctgc caccgtgtat ttctggtcaa caaccagttg agcggattcg cgtttaaact
       61 ctgcgctaaa atttcttttt ttcattagag cacctgtgtt gttctgaggt gagcatatca
      121 cctctgttca ggtggccaaa ttcagtgtgc cacttcacag tgagcagctc tggtgaagga
      181 ttttccagcg tcatgttacc gtgacggaac tccactttgc aggagctggc actgacagta
      241 gtctgagtgg ataaggatgg agtaagagca gccatcggtt ctttcggctc atcaggcgtt
      301 atctctacag gtaataattc aacgcctgcg tcagaagtgg ttgtcaccgg aagacgccgt
      361 gatatacgcc cttcgttttg ccagagtctg agccatttga aaataacatc atcattgacg
      421 ccattttctc gtgcaatctg ggccatacaa gcaccgggtt gtgatgccag ttcaaccata
      481 cgaagtttga attcattcga atacttttta cgaggttctt ttcgccagtc ctgtaattcc
      541 atacttggat gtccgtctgt gtcagatggg cgtctaagtt accaattctt gtctaatggc
      601 tacatactgc ggtcggttta cgcttacttt taacgtaaaa accgtgatgg caagcctgtg
      661 ccgtcacgga ctgagggcaa aggcttcccg gaagttcagc ccttaccgca cacagcctgc
      721 ctgtgtcaga aaatctgctg gagctgtcaa cgacggatga aaagtgcctc ttccgcaacc
      781 tgggtggaga ttttcatgcc agtggactca ttaccctctg cctgagtagt tcaaatcctg
      841 ctcgaccata catctggcgt ttcagcatct tcaggcgatt tacatgacct tcgacaacac
      901 cattactcca gcgactactg attgcctcac atatagccgc agcatcagct tccatccccg
      961 cagctacgca ccgaagttct gctgagccgc tttcgtggac tcgagtgaac cagctgctta
     1021 gctgtgattt attttaggtt ttcagaatac ggtagaactc gagtaccagt tgctgtgcta
     1081 ttttcaactc cggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     1141 catccctggt tattctccag ggcatcaacc agcggcttac ccgggccacc atttcccgcc
     1201 atatccggct ggcgttataa ttaccacttt ctcgttgctc ttttaaccac tccctccatg
     1261 ggtccagaag ccctcgcttt ggaggtcggg ttgacatttc agggaatgtt cctgaacgca
     1321 cccagcgact gactgtcaca cgcgacaggc ctgtaatacg ggatatttcc ctgaaactac
     1381 atcccttgtt atgctgggcc ataacctctg tccaacgctg atgccgtttt ttgcgggttt
     1441 gttgttttag gcgttccgga cgacggagcg atgctgcagg ctcagacagt tctggctcag
     1501 gtggtttctt tggtgacaac tcgctggcaa caagacgtat cagaggtata tgtctgtaca
     1561 tcattcgttc aagcgcatcg ccaatatttt ttagcaggtg gcatcgacca cctgtctggc
     1621 ctgtggtaca ccttcacgtg ctgctgtcgc acagacccca ctgcgatcac gcgagacaat
     1681 ctgtatttcc ggatattttc tgaaccaggt cgccagcgta cgctgatcac ggccgggaag
     1741 caggacgagg ggacggtgag tatcaagatt gacgattaac gtaccgtaac ggtggccccg
     1801 atgccacgcc cactcatcaa taccgacatg aggcgcgcct gactgtttcg tctccggggt
     1861 attgataacc ctgcgaagga gagtatcagc actgcaacgg agacccactg ccgttgctgc
     1921 ccgttttccg gcctcacctc cggcgattaa tcccagttga cgctgtaaat tttgtaacgc
     1981 ctgtgaagac tgcagatgtg aaccaccgaa gggaacaaac gactcggcaa aaatttttcg
     2041 tgaacaagcc gggttacggc tgtacctgtg gcggacggaa aatacaagcc atagcgtctg
     2101 ccctgagcag ggtagatgct gtatccggcg ccgacgacaa ctatgaacag agtcgctacg
     2161 cttaaagcat tcaggacatt gtgctgtttt gcgttttccg cggagatgga ggggtatacc
     2221 gtcagagtta ataatttgtc gactacatcg ccatccgcaa ggtaactgta gaagtgtttt
     2281 aagagagtgc attgcgttac ccataactaa aaacagctta gatccttgtt caccataaag
     2341 tttcatccat tttctccacc caggttgcgg aagaaccact tttgcaccgt tcgtgacagg
     2401 agcaggattt ttacgccagt agccagagcc agaagtgggc gcgagacatc acgtacttac
     2461 gtacggatga aggctggctg tatctgggag tggttaatgt cgccacgtat gtctgcataa
     2521 gagacctcgg aacgttatgc taccgcatgt catttgcaaa aatgacagat agatatactt
     2581 caatttaaac cttaacaatt agcatgggaa tgctgtcaat cctctgtatg caaaaatggc
     2641 atctgaatcg ctcattattt agccctctgt gtcaaagcgt aaagtgttcc cagagagcct
     2701 gtacatagat tcgtgtaatt tcctgatttt gatatgttca tcgacaggaa tttatccccc
     2761 ggaaagatta agaaaacaaa aaacgaacat ttattcaaca catccggaac ctcatcgggt
     2821 atatatcagc gcttatattg ctgacattga ttgatacagg gtatcggtga cgtttttttg
     2881 ctccatttcc ctcaaaatgg acgcaacttc ccctctgcgg ctctcagccg caatactgca
     2941 ttcgggccag cggctcatgc atcataacca gctctgccag acggtaaccc cgcttcagcc
     3001 ccgtaaaccg catctgactt tcgcacagga tgcatttcag cgggtcaacc ttcagtaacc
     3061 gctgatacat ccctctccac ctgatttgca tcgccgtttt tatcaccgtc tctgttatta
     3121 cgtacactaa ttcttccagc aaccggcgtt tcgccggact aaggaacccg tagaacctca
     3181 ccatacggaa ccccttatcc gccacatgcc aggagaacct ttccataaac gcatcaccac
     3241 tcatcaatag gtattcttca cgtcgtgtgc ggtgactgtt gtaacgcagt ccgatttcat
     3301 cctgaccggc atagtgctcc agacggctca ttggcaccgg cggctttttc aggtaagagc
     3361 caaagtacac cgccacatgt gtggcgttat ccatcacctt tgatacgttg acattcctgc
     3421 cccgtcggta atgactgtcc aggaaacgat tccattcccg tttactgctt ccttcagcag
     3481 ccagctcatc cggtatcaat agttccgggt atttccgtga cagtaaccgt gttatccggt
     3541 atcgctacat actcatcacc ttacgggcgt aaaaatgaag gtttttccag gtgtgatgtg
     3601 atgtcacacc gccggcagtt gtcgataaat ggataaatgg atatgtggat atgtggatga
     3661 cactgctggt cccggcccca tgtgtggatt accgtgaata tccccggctt cacatctgct
     3721 gctatgcggc tcatctctgc cagtaaccac cggttgtgga acaccaggga ccagtactgg
     3781 cagggaagcg tgaatacaat tgctgccacg ggccgtcggg gaccagactc agcagatact
     3841 gtatccactg tgcacccgct ttcaccccgc agtgtgggca ggagcgtttt ttacaccgaa
     3901 aacagacctt ctttgtattg caacagtccg ggcagtacat gccgccggcg ctgataccgg
     3961 caagaatggt cgtaaactcc cgctctgtac agcgggcgat ttccggatgc ccttcgtcat
     4021 caacacgtac aaaccagaag accagctttt tgtttccggc atccacaaag aacggaatat
     4081 tcaggtctgc gcaacactca acagtatcgt caaaattatc gaagcgcaga atctctactt
     4141 cttcttcgtc aaaaaaatca tcttcgtgaa gttttacaac atagcgggga agtttgcttc
     4201 tttgaggtgc gagttttcgt ttacggggtt tagctgaacg tgccatataa ctacctgaaa
     4261 gacaatgaca ttgtttgttt ttataacggt aatttcaggc catgacaagc cgcagccgct
     4321 aggctgccta ctcgatcgat aggaatttaa atcccaaaaa gattaaaaaa acacccaaaa
     4381 acggatgttt attcaacact gcctgcgtgc catgagtgca ccgccgtcaa ttaaatggat
     4441 cttatcgatt gatactgcat atcactgatg gttttttacc ccattcccct gaaaatggac
     4501 gcaacttctc cgatgcggct ctcagcc
//
LOCUS       DAZFJF010000238         4470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFJF010000238 DAZFJF010000000
VERSION     DAZFJF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.240"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..523)
                     /locus_tag="ACHKAU_004960"
                     /pseudo
     CDS             complement(1..523)
                     /locus_tag="ACHKAU_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(483..602)
                     /locus_tag="ACHKAU_004961"
                     /pseudo
     CDS             complement(483..602)
                     /locus_tag="ACHKAU_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(615..2225)
                     /locus_tag="ACHKAU_004962"
     CDS             complement(615..2225)
                     /locus_tag="ACHKAU_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO5533844.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSNGSMTLMLKEMSNLINDEGIK"
     gene            complement(2900..3889)
                     /locus_tag="ACHKAU_004963"
     CDS             complement(2900..3889)
                     /locus_tag="ACHKAU_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533845.1"
                     /translation="MTMTKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALALAK
                     AVKTMLNGVLKSAQDNIPFPIEILPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTEPSFGSEVADYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFGCFRHDGDHITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(3964..>4470)
                     /locus_tag="ACHKAU_004964"
     CDS             complement(3964..>4470)
                     /locus_tag="ACHKAU_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533846.1"
                     /translation="SSKASALFNNQDWKLSVTDINLYENTVSLDGQFYPLSLAIKTLI
                     PGYLSGLPSTSRESMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQ
                     RIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLES
                     GGNWMDNL"
BASE COUNT         1164 a         1032 c          966 g         1308 t
ORIGIN      
        1 tttactaaat ccaattacaa aatgaatgga ccaatataat gattttttgt ctaacactgg
       61 agcgtttccg tattggcaga ctgtgacttt atctaaccaa agattcaatt cccctgttca
      121 tattcttatt agtggttgag cagggctcaa tactccatca aatactttac ctacgtgcaa
      181 acgattatat tttagttcag gatgctttag atttaacggg gaactggata aactgagcca
      241 ttcccctatt aacctttccg tttctggcat taaagccatc ccagcgagct cagatgaaat
      301 taagggaaat atttctccaa gcaataccca tccagaccag ctgccactct cttctgctat
      361 cggtaaccat ttagcactaa attcgggcat taataatgca acatcttcgc cattttcttt
      421 ccaattttga aaagtggttt caaacgtatg agtgtttcga tttactttcc tcagaccgaa
      481 cattaacttt catccttctg atcgttttcg aatttgcttt cattttcttg ctcctcataa
      541 ggctgatgtc gttgtccttg tcgttcatct tgctcagtca atacccaacg ttgtgctgta
      601 actcattatg aaaactattt aattccctca tcattgataa ggttggacat ctctttcaac
      661 atgagagtca ttgaaccatt cgaccaattt agcggaatga tagggcttgg cgcttcatgc
      721 aaagttcccg acttgtggat atagttatag ctttcgggaa gtgccatttc aggtacactc
      781 ctgccatttg caccaatcat attctcgcct gttatttggg ccaatgatct attcataaat
      841 tgcactgagt cattcaaata ctcttccttt cttgattctt tataaacaat tgctgctgat
      901 tttgcatacc atgagtcaaa aaaccattct gcttctgttg atggaatgaa gctcttttct
      961 cttttcgcat gtgaattttg atcggtatcg gttttaatat cattgaacca aaaatttgcc
     1021 gactgataat tatctttctc gtatcttttt ataccatacg gacctgctag ttttttaact
     1081 atttttagaa cctgctcttt tcttctggtg tttatctttg caagatttgc aggataaata
     1141 acattaagta aagccgcatc ggcttctctg taatgtttat ctttttccag ataacctggg
     1201 gattctcccc ctaaatcaag ctgtaacgtt attctttcat aacctttatc tatgaggtgg
     1261 ttcaacctcg ttgttgaaag aggttcatcc agttcattgg ctttagcttc gcgtagtaaa
     1321 tcagagacaa aaacagagtc tttcttcgag agtaagttcg acaaacgttc cagtccagag
     1381 gtcaccagtg caacggaaga tgtgtttaat cgggcatctt cttcccatgc tccggaatct
     1441 tccataacat agaaattggc tttatccaga taagcaatca gcagcgcaac cgattttaat
     1501 cggtcacctt tttgccaatc ttcagcgtta attgtatcag tgtcgattgc ttgtataagg
     1561 agatcaagat aaagacctaa cgcatcattt tgtttatggt tccacagctg cggcttgcct
     1621 tcttcctgca catctgccat cacgggggag ttgctgtcaa atcggatatg cacaacattc
     1681 atttgcccgg gaactccatc cagtcgtttg gggttgctaa tgacgtcctg catccgctta
     1741 atctggtcgg gcgtagacat ataatcccac agggtgagca gaaccttttt tgcggctaca
     1801 ctatttccct gatctgatac taatgccata tagccccata aactatccct cagccagaca
     1861 gcatcatagt gggtttctgc cgctgttttg tcatcttcga aagtagaagc aagaataaac
     1921 gttccccagg gtttctccgt caacgttaac tttcgtgtgt tttctgttaa tgttagcaat
     1981 ccttgaattt cgtctttggt atagtggctc tttatttcat tttgtaattt ggctgagtga
     2041 atcaaaagac gataattagg atacttatct ggatttaacg ataaataact tttatctgtt
     2101 gctgcccccg tggaattaag aatgttaaat acagattcac tcgtcacttc tgcccctgtc
     2161 gcctggcatg tgccgcacga aaatatacca gcgagcagaa gagttaatgc agatttccct
     2221 ttcataaaat attccatcaa tcgtttgtta aaagcgtaga taaccactta tttaacaact
     2281 tgaaaataag gtgttttacc tgggttgtta caaaaggatt gcattgcgta aacgcttttt
     2341 atttacaaca aaatggggaa gtattacggc gattgcgaaa agtctgaagc gattgaatta
     2401 agataatata gaaaatcaga tacagcaaaa atggcagatt gatcaggtga agataaattg
     2461 gagcgggcga agggagccta cgacaaatcg aagacaacca gctaacctct tgtttcttaa
     2521 agactgatga atccagttgg ttgtagtgat agtcacacat ttagcgatag acggtcaaga
     2581 cttgaagcac gtaatattcc ctctaaagga atagaactca cggtctgact acccggccct
     2641 tttggtctga ctcagggtcg ggctatgggg acttttggct cgctctttat attagatgag
     2701 gtctcacatt tttcagtcaa aactcatcgt ctaatagccc atagccagac caccagtccc
     2761 agctttagca ggaagaccac gagatagact ttgtgtaggt ccatttggtc ctcctccctt
     2821 ctgtattcac gaactagatt actagtacaa aataaaggcc agacatccag ttcggaagtc
     2881 tgaccaatag tcgttattgt tagttagctt ctttagtgaa gctcaggcgt ttgactacag
     2941 gcaggacagc agtagccgag ccagtcccca tcttgatggt cgtagcagct tcagctacaa
     3001 caaacacctt gccgagaatc tcaaggtcgt gagactggga gtcaccatca cgtgaaagga
     3061 ccacagtaat gtggtcccca tcgtgacgga agcaaccgaa gctcagagcc gtaaaacgct
     3121 tagccatcat gaagacgcca cggtcttcac cgttgtaata ggcgatgtca gtccccaaca
     3181 tatcgacgat agcgtcttca ccaccaaagc cctgcttagc agcaagacgt gacagtgcct
     3241 tgaggtgtga ctcatgggcg atgatgccgt aatcagcaac ctcagaaccg aaagaaggct
     3301 cggtgttctc gtacagcgcg tccatgatag tgtctgcctt agctgtcagg ctaccagtca
     3361 ggaccggaag ctctacagtc ttcaacagct tggaggattc cagtacatcg accatgcgac
     3421 tattgatggt acggaccacg ttatcgcaga tgttcagagc gtcctgctgg agcgtcccct
     3481 gagtgaaacc agtggaagac ttagcgtccc atagcttggt ctggtgggac acgaggatag
     3541 cttgctcaat cggctcaacg gtgatttcag taccagacgg agcggtcacg acttcaggtc
     3601 gcttaccacc acggacagga acgaccacag agcggtcagc cagagtgtga gcaatgattg
     3661 gggtcggtaa gctattgcga gttggcagaa tctcaatcgg gaatggaatg ttatcctgag
     3721 cggacttcag cacaccattc agcatggtct tgacagcttt agccagtgcc agagcataga
     3781 cctgtgggtc agcattggtt ggcagacctt cgttaacctg aatgttgtct gtgtcggtga
     3841 tacgtactgg ctcaatgccg aactgatagc tgtctttagt catagtcata ataatcaatc
     3901 tcctaaatta gtttagatac gtgggtcccg acatcaggtc aggaccactt gtgtgcgtct
     3961 gtcttacagg ttgtccatcc agttaccgcc tgactcaagg tcagcgacga tagcggcacg
     4021 ggagcgacca gcggaatggg tagcactacg aaggttctca ccgtaggtcc ggcgttcagc
     4081 tttgacctgt tcacggcgct gtagctcttt ctgccactct gcatcagtca tgttctcttc
     4141 acgcatgcgc tcggcctgaa tgcgctgctc tttagcgata cgctcagcct cagctcgctt
     4201 gttctgctgg cggcgaccat aggcggtcat caggtcatta ctgaagaagt taccaatggt
     4261 cacgccagct tcagccaggg cttccaataa ctccatagac tcgcggctgg tagacggcag
     4321 gcctgacagg tagcccggaa tcagggtctt gatagcgagg gacagcggat agaactgacc
     4381 gtccagactc acagtattct cgtagagatt gatgtcggtg actgacagtt tccagtcttg
     4441 gttattgaat aacgcactag ctttgctaga
//
LOCUS       DAZFJF010000239         4440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFJF010000239 DAZFJF010000000
VERSION     DAZFJF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.241"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            495..755
                     /locus_tag="ACHKAU_004965"
     CDS             495..755
                     /locus_tag="ACHKAU_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5533847.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            752..1300
                     /locus_tag="ACHKAU_004966"
     CDS             752..1300
                     /locus_tag="ACHKAU_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5533848.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1300..1524
                     /locus_tag="ACHKAU_004967"
     CDS             1300..1524
                     /locus_tag="ACHKAU_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533849.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1521..1844
                     /locus_tag="ACHKAU_004968"
     CDS             1521..1844
                     /locus_tag="ACHKAU_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO5533850.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1859..4192
                     /locus_tag="ACHKAU_004969"
     CDS             1859..4192
                     /locus_tag="ACHKAU_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO5533851.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
BASE COUNT         1068 a         1040 c         1140 g         1192 t
ORIGIN      
        1 aaacattcaa cctccctgca ctcagttgca tttcaggaaa atattcataa aaatgaattt
       61 gtctgatgtt cattaaacac atccggatac atgcggaagt aagatggtaa tttaatttct
      121 ttaatcatcg gatttattga tacatatggt gagctgttta tttttggccg ttttggctct
      181 tttattaatc aggtgttgtc ttagtgctta tgatgtaacg ccctgaatga tatgggctgg
      241 ttcagatttg agctgtgatc ctgcctagtg taatagactt ttttattatt gatgctcctt
      301 attatattcc ataaaggaat tgtttgtttt tgttcgtttc atacggtttt tagctgtttt
      361 attttttgag aaatcaacat ttcacacaaa ataaataaag catgcgcgag ccgcttatat
      421 aaacataata aggagctacc tgaatccgca tggaattgcc cggactgttg taaacatatt
      481 agagaggtag ccagatgaat agtatttccg tttccacccc ggcaccagct gctccggtgc
      541 tcccggttcc ggctcagcag gagcgcttca tacgattacc tgaagtgatg cacctttgcg
      601 ggctatcccg ttcaaccatc tacgacctta tcagccgcga tgccttcccg cagcagatat
      661 ccctgggtgg aaaaaatgtg gcctgggtac agtcggaggt cagtgcatgg atggcggcgc
      721 gaattgctgc ccgcgagcaa ggaggccatg cgtgaaactt gctttgcagt ctcatcttct
      781 tttttctggc ttgcgtcctg atatcttttc caggtatagt tttcccgctg ccgcaaaatc
      841 ggctgccggg cgtaggaacc cgagttactt attggcgaca ccagacgcgc cgtgcgtctt
      901 tttttacgtc gttgctcagg cacacccatt tttcgggctg cggtgcttat accttagccc
      961 ctttcagata atggtggtcc gggcggggca gccttcgggc tggccggttt ccaataaggc
     1021 cggtattcct acccccgttc gggtcaccac ccatgagcgt aggaactccg gtggtggcat
     1081 ttaccgctac ttattggagg ttgcccttat ggctacgatc ctcgccccgt cacatcctca
     1141 atttatattt gtttttgccg ccgttcgtcg tgcagaacgt aaaccacgta tcagtatgct
     1201 gcgtatcatc gccagcaatg agcatcatgc acgtctttca ctggtccatg agtatgtgct
     1261 ttgtcttgct gctcgcctgc ctgtccggga ggtgatctaa tgaatcacgc cgcaatctct
     1321 tatgacgata ttctccgcct gaagcatctt cgtaacgtgg gagagtttgt cactggaatg
     1381 gctgttcttc aggattgcta cgaaaaacct gccagtgctc aatgtgaaca actggtttcc
     1441 ctgatttatc tgatgacaga gcagcttgat ggcgtggtac agcgttgcca ggatgacctc
     1501 atgaacatgg aggtggtgca atgaaatcac gcactctttc cctttcactt cgggtagcgt
     1561 tgtatcgccg cgcggtagcc tgcgcctggt tgagtgcctg ctatcagcaa aaccgccatc
     1621 tgcagctcac tctggatgaa attgaaaccg ctattgcccg tgagcttgaa ggattttatc
     1681 tgcgacagca cgggcaaaca aaaggcatgg aaatcgcctg cgccctgctt tcagacctga
     1741 tggaatacgg ccctctaatg tcctgtcccg cactctccca gctcggaata gccgtaatgg
     1801 atgagttatg cgtccgtcac atcaaaaagc cggttttaca ctgagggaga actgcgtcat
     1861 gtcaggaatg aaagtcagtc aggccgtgaa ggctgcccgc ggtcactggg ctcagattct
     1921 gccagcgctg ggcgtgaata tattgaaaaa tcgccatcag ccttgcccgg tctgtggcgg
     1981 taaagatcgt tttcgcttcg acgatcagga aggacgcgga acatggttct gcaatcagtg
     2041 cggagccggg gatggtttag cgcttgtcac cagggcactg aatgtggata tcagtgaagc
     2101 agctgacagg atacatggac tgacacatga tctgtctgta gttaattccg aggtcaggac
     2161 gttaaccgcc gataccgata gcgggaaaga tgcagcggca gcacttgccg cgcgactgct
     2221 gcaagcctcg cgtgaatctg ccggaaacac ttatcttaca cataaaggtt tcccggagca
     2281 tatttgccat gagctgactt cagctcataa ggccggtggt atgatgttcc gccccggtga
     2341 tttgatcgtc ccactctata acgctgacgg ggagctggtg aatattcagc tcatcagtgg
     2401 caatggtagc aagtgttttc ttaaaggagg tcaggttaag gaggcctatc atctgataga
     2461 agggggcggg agttctgtaa gaaggatttg gattgcggaa ggttacgcca cggcgcttac
     2521 cattcattat ttgacaggag aagccgtcat ggtggcattt tcgtcggtca actttctttc
     2581 tctgagcagc gttgcccata ataagtatcc gggatatcag ttaattattg cagcggaccg
     2641 cgatctgaat ggttcaggcc agaacagggc tgaaactgct gcaaaagcga gtcagtgtga
     2701 cattgtgctg ccaccggttt ttggtgactg gaatgatgcg cttacccaat acggagagga
     2761 atccacccgg aaggcaattc ttgaggcttt gaagccacat aacgccagtc ctttcgacac
     2821 aatgagtgaa gcagaattca cagcgatgag tgtcagcgaa aaagctcaga gggttcggga
     2881 gcattacagg gatgcactgg cggttgatcc gaacgggcag cttttatccc gctacgagtc
     2941 tggagcgtgg aaagttattt ctcagtcaga ttttgcccga gatgtcgcag cccttttcca
     3001 gcgcctcggg gcaccgtttt cctcaggaaa aattgcctca ctggtggaaa cattaaagtt
     3061 aattgttccg cagcagcaaa atccgtcacg tcatctgatt ggttttcgta atggcgttct
     3121 cgatacgcga acagggcttt ttagtccgca ctgtaaagag aactggctgc gtaccctgtg
     3181 tgaagtcgat ttcacgccac cggtaaaggg ggaaacgctt gaaactcatg ccccggcatt
     3241 ctggcgttgg ctggaccggg ccgccggaca caagccagca aaacgcgaca ttattcttgc
     3301 agcgttattt atggtgctgg cgaaccgcta tgactggcag ctctttctgg aagtgactgg
     3361 ccctggtgga agtggcaaaa gtattctggc tgaaattgcg accatgcttg ctggagagga
     3421 taacgccacc tctgcgacca ttgaaacgct ggagtccccg cgagaacgtg cggctctgat
     3481 aggtttttct cttattcgcc tgcctgatca ggagaagtgg agcggcgacg gcgccgggct
     3541 taaggctata actggtggcg atgcagtatc tgtggacccc aaatatcaga atgcttattc
     3601 aacacatatt ccggcggtta tcttggccgt aaataataat cctatgcgct tcactgatcg
     3661 cagtggtggt gtgtcccgca ggagagttat tctgcatttt cctgagcaga tagcaccgga
     3721 agaacgcgat ccgcagttga aagataaaat tgcacgagaa ctggctgtga ttgttcgtca
     3781 gctcatgcag cgtttcagcg atccgatgag cgccagaaca ttgcttcagt cgcagcagaa
     3841 ttctgatgag gctctcacca tcaagcgtga tgctgatcca gcatttgatt tttgtggcta
     3901 ccttgaggca ttacctgaca ccaacggtat gtttatgggg aacgccaata ttgtcccacg
     3961 ccagccacgt acataccttt accatgccta cctcgtatac atggaggcta atggctataa
     4021 aaacaccctg agtcttacga tgtttggcaa agggttgcca gtcatgctga aggagtacgg
     4081 gctacattat gagaaacggc gaactaatca gggaatgcaa actaacctca ctttgaaaga
     4141 ggagagtaat gcagactggc tgcccaagtg tgatgagcca atattaaaat aacgattaac
     4201 taacccggct gatgccgggc ttcttattgg aaacgcacaa atgtagagat agctgttcac
     4261 tcttcacctc gctgttaaca cgtaaacaat tgatatataa gttaaaaatg gcagggtgaa
     4321 tagtgtgcat ggttttgcga aaaaaaagta ttttttgtag agtcaagtcc cggtttggat
     4381 ccgtttaacg gaacctaact agattgacta tttctctgat gatttttgga taaataatca
//
LOCUS       DAZFJF010000240         4408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFJF010000240 DAZFJF010000000
VERSION     DAZFJF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.242"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..345
                     /locus_tag="ACHKAU_004970"
     CDS             <1..345
                     /locus_tag="ACHKAU_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5533852.1"
                     /translation="GNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGTFGAFDNEWH
                     TLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRNATYPVLIDS
                     IVVEVNSTDTAA"
     gene            481..660
                     /locus_tag="ACHKAU_004971"
     CDS             481..660
                     /locus_tag="ACHKAU_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5533853.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDVWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            701..973
                     /locus_tag="ACHKAU_004972"
     CDS             701..973
                     /locus_tag="ACHKAU_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5533854.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            1050..1265
                     /locus_tag="ACHKAU_004973"
     CDS             1050..1265
                     /locus_tag="ACHKAU_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5533855.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1270..1614
                     /locus_tag="ACHKAU_004974"
     CDS             1270..1614
                     /locus_tag="ACHKAU_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO5533856.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1665..2198
                     /locus_tag="ACHKAU_004975"
     CDS             1665..2198
                     /locus_tag="ACHKAU_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5533857.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GTGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            2469..3038
                     /locus_tag="ACHKAU_004976"
     CDS             2469..3038
                     /locus_tag="ACHKAU_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO5533858.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            3038..3184
                     /locus_tag="ACHKAU_004977"
     CDS             3038..3184
                     /locus_tag="ACHKAU_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533859.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            3412..3597
                     /locus_tag="ACHKAU_004978"
     CDS             3412..3597
                     /locus_tag="ACHKAU_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5533860.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            complement(4022..4249)
                     /locus_tag="ACHKAU_004979"
     CDS             complement(4022..4249)
                     /locus_tag="ACHKAU_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5533861.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            4291..>4408
                     /locus_tag="ACHKAU_004980"
     CDS             4291..>4408
                     /locus_tag="ACHKAU_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5533862.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKR"
BASE COUNT         1172 a          974 c         1201 g         1061 t
ORIGIN      
        1 gggaataacc tgctggcagc gttctacatc cagacagatg caaaagacct gaatgtgatg
       61 taccacaatg cgaaagtggc gacaaacaac ctgaaactgg gaacctttgg cgcatttgat
      121 aacgaatggc atacgctggc tttccgcttt gccgggaata acagccttca ggtgacgccg
      181 gttattgatg gtcaggatgg cacaccgttc acgctgacgc agtcaccggt cagtgcattt
      241 gcggcggata aactgcatgt gacagacatt accaggaatg cgacttaccc ggtactgata
      301 gacagcattg tggtggaagt gaacagcaca gacactgcgg catgataaaa aaaccgccag
      361 cgacaggaat ggacgctggc ggtggtgata cctatggaga aaaaataaag gaacgatact
      421 ttcgtactct ggttttttaa tgaaaacagt tcttattgtc aacaataacg gaaagaaatt
      481 atgacatttc tgaaccagtt aatgctgtac ttctgtacgg tggtctgtgt gctgtatctc
      541 ctttcgggtg gataccgggc catgcgtgac gtctggcgca gacagattga caaaagggcc
      601 gctgagaaaa tcagcgccag tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag
      661 cggcaacttt cttaatcaca tctttcgacg agaaaatccc atgtcagaaa ttacatccct
      721 ggtcactgct gaggcagtga aggaagtcct gcgctctgaa gaagtcctga gcgcactgaa
      781 acaaaaactt cgccataacc tggaagcgcg tcttgatgca gaggttgatg ccattctgga
      841 tgagctgctt ggtgtacagg cagagccacc gactgaagcg ggagatacca ccgcagagag
      901 cggtgaagtt cagcctgaat caccggtcgc cgatgcgact gaacctcaac ccgaatcggt
      961 catgatgctg taacggggag tcagggccat cagtaaacag ctgctggcct ttttcatgtt
     1021 gtgagcttcc ggataacggg agacggggta tgtaccagat ggaaaaaatc acaacaggtg
     1081 tgtcatacac cacgtcagcg gtagggacgg gatactggtt actgcaactg ctggacaaag
     1141 tctctccgtc ccagtgggtg gcaataggtg tactggggag tctgctgttt ggcctgctga
     1201 cgtatctgac taacctgtat ttcaaaatca gagaggaccg gcgtaaggcg gcgcggggag
     1261 agtaaagcga tgaagaaaaa atacgaactg gttgttaaag ggataaataa ttacccggat
     1321 aagattactg ttactgtggc actggaaatt ggtgggtatc cgtcactgtt gttgccagat
     1381 gtggcgatta gtcttgaccg tactgaaggt gccacgctgg agttttacga agctgaggcg
     1441 aaaaagcagg cgaagcagtt tttcatggat gttgctgccg ggttatgtga aggggatggt
     1501 ccgttgccgg aaaagcggcc catcatttta gaggcgcagg atgtgttgat aacctacaga
     1561 ggaaaactac cgggaataat tactggttct ctgaagactc caccgctggc ctgaagactt
     1621 aacatatcca gggatttgaa atcgataaac cctgataaat atccatgaac accaaaatca
     1681 aatacggcct gtcggctgcc gttctggcgc tgattgccgc aggtgcgcct gcacctgaaa
     1741 tcctcgacca gtttctggat gaaaaggaag gtaaccacac cacagcatac cgtgatggta
     1801 cgggtatctg gaccatctgc cgcggtgcca tcctggtgga tggtaaacct gtcgtcccgg
     1861 gcatgaagtt gtcgaaggaa aaatgcgacc aggttaacgc cattgagcgt gataaggcgc
     1921 tggcatgggt ggagaaaaac atcaaagtgc cgctgaccga accccagaaa gcggggatcg
     1981 cgtcattctg tccgtacaac attggtcccg gtaagtgttt cccgtcgacg ttttacagac
     2041 gaattaatgc aggtgatcga aaaggtgcct gcgaagcgat tcgctggtgg attaaggacg
     2101 gtggcagaga ctgccgtatt cgttcaaaca actgttacgg tcagatatcc cgtcgtgacc
     2161 aggagagcgc gctggcgtgc tggggaatcg acagataaga agaatatttt gctgaaaaat
     2221 gaggtttgct tacatggacg gataacacga aatcctgcaa attggcaaaa tgtaagtgaa
     2281 taaagtcaaa acagttgttt aacactcagg caccgtaatg atgcctttgt catttctgcg
     2341 catctcacgc gcatctcaca acacagaacc tttcaggatg acccttgagg ataccggttt
     2401 ggctgtcggt gcctttctgt gggctggatt cctgtgagac aaggttcatc actaaaagga
     2461 aataaccgat gaatatgatg gccgtgccgt ttcacggcaa ctctctttat gtagttaacc
     2521 ataatggcga accatacgtt cccatgaaac ctgtcgttgc ggggatgggg ctggcctggc
     2581 aatcacagtt ggctaagtta agacagcgtt ttgcgtcaac tataacggaa atcgttatgg
     2641 ttgctgagga tgggaaacaa cgcaatatgg tgtccatgcc acttcgaaaa cttgccggct
     2701 ggctacaaac cattaatccc aacaaagtaa aacccgaaat ccgcgataag gtcatccggt
     2761 atcaggaaga gtgcgacgat gttctttacg agtactggac gaagggtttt gtcgttaatc
     2821 cccgtaaaat gagcgtgatg gaagaactca accaggcttg tgctgacatg aaacgggata
     2881 aaaacattgc cagtgtgttt gctaccgggc tgaatgagtg gaaacaggtt aaagccgcgc
     2941 atgtatcaaa aatccgtacg ctggtaaatg aagcgaatat gctgattgat tttgtcctgg
     3001 ctgatacagg caaagggaaa ataacaaagg cggattgatg gggtggctaa tgatatcaga
     3061 taaactcata acgctggtga agagcctctg tgtacttgtc ggcatttcat ttttagtcat
     3121 gctggttgcc attttctttt ccaccgcctg gcgagtcctg acgttatcgg gactggtggg
     3181 gtgaaagaga gatgaaccgt gttctgtgtg tggtgattat tgtcctggcg gttggctgtg
     3241 gtgcgctgtg gctggcaaca aaccattacc gtgacaacgc gctcacctac aaagcgcagc
     3301 gcgataaaaa agccagagag ctggaacagg cgaatgccac cattactgac atgcaggtgc
     3361 gccagcgtga tgttgctgcg ctcgatgcaa aatactcgag ggagttagcc gatgcgagag
     3421 ctgaaaatga aactctgcgt gctgatgttg ccgctggtcg taagcgcctg cggatcaacg
     3481 ccacctgctc cggtaccgtg cgtgaagcca ccggcacctc cggcgtggat aatgcaaccg
     3541 gcccccgact ggcagacacc gctgaacggg attatttcat cctcagagaa cggttgatga
     3601 ccatgcagaa gcagctggaa ggggcacagg actatatccg cactcagtgc ctgaactaag
     3661 ttttgctgat gcgccgtatc gtcgccgtat tcctgcatta acagagaccg cagcccgaca
     3721 gggagactcc tctgcgagag tgtgcgggga taatcaaaaa cgatacacac cggggtttac
     3781 cgcgtaaacg gagcgcggcg ttctcccctc atggtcgccc gtccggtgcg atggtggaag
     3841 aaactggaat ctgttcaata aaaaaactgc cgtgttggag tcacagcagt aatgtactga
     3901 ttgggtagaa gattattatt gttatgcttt atttttattc tatatggctg attatttcaa
     3961 ttcggaatta atacagctaa tgtctgtgag tttttataaa ttcagcaata taaagaaata
     4021 gttatatgaa cagccatcgc agagcatact gtgtatcatt cttttttata gtcaactgac
     4081 gggcatattt tatgtctgct gccagctccc ggcggcaaga ttcaatgacc cacgcagaaa
     4141 aattttctga acctttctgg tcaagagcga tgttaatttg ttcaatcatc tggtttggaa
     4201 atcggatgtt gcgggttgtt gttctgcggg tccggttttt cgatgacatt ttctttcctc
     4261 tggtgacaag ttatatggcg aggattttac atggctgtgc ttcgtacgtt accgggcaga
     4321 atcaaaactc tgaacacccg gcgggtgaat gtcctgaagg gtgaacagcg tcgggtcagt
     4381 ggtagtgccc gggtttccct caagcgcc
//
LOCUS       DAZFJF010000241         4313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFJF010000241 DAZFJF010000000
VERSION     DAZFJF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.243"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..659
                     /locus_tag="ACHKAU_004981"
     CDS             75..659
                     /locus_tag="ACHKAU_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5533863.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            739..1434
                     /locus_tag="ACHKAU_004982"
     CDS             739..1434
                     /locus_tag="ACHKAU_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5533864.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1464..3980
                     /locus_tag="ACHKAU_004983"
     CDS             1464..3980
                     /locus_tag="ACHKAU_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5533865.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3991..>4313
                     /locus_tag="ACHKAU_004984"
     CDS             3991..>4313
                     /locus_tag="ACHKAU_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5533866.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKT"
BASE COUNT         1235 a         1007 c          986 g         1085 t
ORIGIN      
        1 ataatcttta aagatgttga acatatattc acattaaata tgattatgta cttgttacaa
       61 ggataaggtt ataaatgaat aaagttacaa aaacagctat tgtgggctta cttgcgcttt
      121 tcgcaggtaa tgccgctgca accgatggtg aaatagtttt tgatggcgag attttaaaat
      181 ccgcttgtga aatcaatgac tctgataaga aaattgaagt tgctcttggt cactataatg
      241 ccgaacagtt tcgttctgtt ggcgatcgta gcccaaaaat tccattcact attcctttag
      301 ttaactgccc agtgactggg tgggagcacg ataacggcaa cgttgaagcg tctttccgtt
      361 tatggctgga aacgcgggat aatggcaccg tccctaactt ccctaatctg gcaaaagtgg
      421 gctcttttgc tggcacagcg gcaacaggtg tgggtatccg tattgacgat gcggaaagcg
      481 gaaaccttat gccactgaat gctatgggga atgataacac ggtttatcag atcccagcgg
      541 actccaatgg tattgtcaat gttgacctca tcgcttacta cgtatcaacc gttgaagcat
      601 cagaaatcac cccaggggaa gcagacgctg tcgttaacgt aacgctggat taccgttaat
      661 acgttacggc gttatctgac ctttcagata acgccctttt ccttcctctt tctcgttgta
      721 tcaggttgaa aaataactat gtcaaaacga acattcgcgg tgataataac cttgttgtgt
      781 agcttctgta tcggccaggc gcttgcaggg ggtatcgttt tacagcgaac gcgagtgatc
      841 tatgatgcca gccgcaaaga ggctgcgtta cctgtcgcaa acaaaggcgc agaaacgcct
      901 tatttactgc aatcatgggt agataatata gatggtacaa gccgtgcccc atttattata
      961 accccaccgc tatttcgtct tgaagccggc gatgactcat cactgcgaat tattaaaaca
     1021 gctgataacc tgcctgaaaa taaagagtcg ctgttctaca ttaatgttcg tgccattcca
     1081 gcaaagaaaa aatcagatga tgttaatgct aacgagttga cgctggtatt taaaacacgg
     1141 atcaaaatgt tttatcgccc cgcacacctg aagggacggg taaacgatgc gtggacatca
     1201 ctggaattta aacgtagtga ccattcactc aatatatata acccaactga atattacgtc
     1261 gtatttgccg gactggcagt cgataaaacc gatctcacaa gcaaaattga atatatcgcg
     1321 cccggagaac ataaacagtt accacttcct gcatctggcg gaaagaacgt gaaatgggct
     1381 gcaatcaatg attatggcgg cagttccggg acagaaactc gtccactgca ataacaaata
     1441 taaaaaacac aggtcatcag ggaatgccac aacgacacca ccagggacat aaacgcacac
     1501 cgaaacagtt ggcgctcatc atcaaacgct gtttgccgat ggtgctcact ggcagcggca
     1561 tgctttgcac taccgctaac gccgaagagt attatttcga ccccattatg ctggaaacta
     1621 caaaaagtgg tatgcaaaca actgatctgt cacgtttttc aaaaaaatac gcacaactac
     1681 caggaactta tcaggttgat atctggctga ataaaaagaa ggtttcacag aaaaaaatta
     1741 catttaccgc caatgcagag caacttctgc agccacagtt tacggtagaa caactacgtg
     1801 agctgggtat taaggtggat gaaatcccgg cgctggctga aaaagatgac gatagcgtga
     1861 tcaactcgct tgaacaaatc attcccggta cagctgctga atttgatttc aatcatcagc
     1921 gacttaattt gagcattccc caaattgcac tgtaccgtga tgcaagaggt tacgtctccc
     1981 cttctcgttg ggacgatggt ataccaacgc tgtttaccaa ctactcgttt acaggttctg
     2041 ataaccgtta ccgccagggc aatcgtagcc aacgacagta cctgaatatg caaaatggtg
     2101 ctaattttgg cccctggcga ttacgtaact attctacgtg gacacgcaac gatcaggcgt
     2161 caagctggaa cactatcagt agttatttac aacgtgatat caaggcgttg aagtctcagt
     2221 tgcttctggg agaaagcgcc accagcggca gtattttttc cagctacacc tttactggcg
     2281 tgcaactcgc ttccgacgat aatatgttgc caaacagcca gcgcggattt gccccaacgg
     2341 tacgcggtat cgcaaacagt agtgcaatcg tgactatcag gcaaaatggt tatgtgatct
     2401 atcaaagcaa cgtgccagcg ggtgcctttg aaattaacga tctctacccc tcttccaaca
     2461 gcggcgattt agaagtcacg attgaagaaa gtgacggtac gcaacgtcgc tttatccagc
     2521 cttattcttc attacccatg atgcagcgac ctgggcatct aaaatatagc gcgaccgctg
     2581 gacgctatcg cgctgatgca aacagtgata gcaaggaacc cgaatttgct gaagccacgg
     2641 caatatatgg tttgaataat acttttacgc tgtatggcgg cctgctcggt tctgaagatt
     2701 attatgcgct ggggatcggt atcggcggca cacttggcgc actgggcgcg ttgtcgatgg
     2761 atatcaacag agctgacacc caattcgata accggcactc ttttcatggc tatcaatggc
     2821 gtacgcagta catcaaagat atcccggaaa ccaacaccaa tatcgctgtt agctactatc
     2881 gctataccaa cgatggctat tttagttttg atgaagccaa tacccgtaat tgggactata
     2941 acagtcgcca aaaaagtgaa attcaattca acatcagcca gacaatattt gatggggtaa
     3001 gtctgtatgc ctccggttcg cagcaagact attggggcaa taacgataaa aacaggaata
     3061 tctcagttgg ggtttccggt cagcaatggg gaattggtta cagcctgaat tatcaataca
     3121 gtcgctacac ggatcaaaat aatgaccgcg cactctcttt gaatctcagt attccgttag
     3181 aacgctggtt accgcgtagc cgggtttcct atcagatgac cagccagaaa gatcgcccaa
     3241 cccaacatga aatgcgtctt gatggctcac tgctggatga tggtcgcctg agctatagcc
     3301 tggaacaaaa tctggatgac gataacaacc ataacagtag cctgaacgcc agttaccgtt
     3361 caccttatgg caccttcagt gccggataca gctacggtaa tgacagcagc caatacaatt
     3421 acggcgttac cggcggcgtg gttatccatc ctcatggcgt gacgctctcg caatatctgg
     3481 gcaacgcttt tgcgcttatc gatgctaatg gggcttctgg cgtgaggata caaaactatc
     3541 cggggattgc tactgatccc tttggctatg cagtggttcc ttatctcacg acttaccagg
     3601 aaaaccgtct ctcggtagat actacgcagc tgcccgataa cgtcgatctt gagcaaacaa
     3661 cacagtttgt ggtgcccaac agaggtgcaa tggtagcggc gcgtttcaac gccaatatcg
     3721 gttatcgcgt acttgttaca gtcagcgatc gcaacggtaa accgttgccc tttggcgctc
     3781 ttgccagcaa cgatgagacg gggcaacaaa gtatcgtcga tgagggcggc atactatatc
     3841 tctctgggat atcgagtaaa tcacaaagct ggactgtacg ctggggaaat caggcagatc
     3901 aacaatgtca gtttgctttt agtacaccgg attcagaacc aacaacctct gtattacaag
     3961 gcacggcgca gtgccattaa ggataaaaaa atgaaaagag cgcctcttat aacaggactt
     4021 ttgttgatat ccacatcctg cgcttatgcc tcctcaggag ggtgtggagc tgacagcact
     4081 agcggtgcga caaattacag cagtgtggtt gatgatgtta cggtgaacca gacagataac
     4141 gtgacaggac gggagtttac ctctgccacg ctaagtagca ctaactggca atacgcctgt
     4201 acctgctctg cgggtaaggc agttaaactt gtctatatgg tcagccctgt acttaccacc
     4261 actggacatc aagcaggata ttacaaactc aatgatagtc tggatattaa aac
//
LOCUS       DAZFJF010000242         4278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFJF010000242 DAZFJF010000000
VERSION     DAZFJF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.244"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..264
                     /locus_tag="ACHKAU_004985"
     CDS             <1..264
                     /locus_tag="ACHKAU_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5533867.1"
                     /translation="GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQ
                     LSKRERYSEKWQRRTHSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            261..842
                     /locus_tag="ACHKAU_004986"
     CDS             261..842
                     /locus_tag="ACHKAU_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5533868.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            1185..>4278
                     /locus_tag="ACHKAU_004987"
     CDS             1185..>4278
                     /locus_tag="ACHKAU_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5533869.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDVEGYLDFFKGKITESHLGKELLEKVELTEDNASRLEEFSKEWKDAN
                     DKWNAMWAVKIEQTEDGKHYVAGLGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGNVNANSGT
                     LNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPF
                     D"
BASE COUNT          987 a         1119 c         1332 g          840 t
ORIGIN      
        1 ggcttttacc gggtgcccct gtcctctgca caggcgggcg atatcctgct gtgctgcttt
       61 ggcgcatcgg tggccaatca tgccgccatt tactgcggca acggtgaact gcttcaccat
      121 ctgcctgaac aactgagtaa acgggagagg tattccgaaa aatggcaacg acgaacgcat
      181 tctgtctggc gtcaccgcca ctggcacgca tctgccttca cggggattta caacgatttg
      241 gccgccgcct cagcctgtat gtgaacacgg cagcggaagc catccgggcg ctgtcgttac
      301 aggtgccggg ctttcgccgt cagatgaacg aaggctggta ccagatacgt attgccggtt
      361 atgacacggc accggaggcg gtgtacgccc gtcttcacga acagctgggt gagggaacgg
      421 tcatccatat tgtgccgcga ctggccgggg ccggaaaggg tggactgcag attgtgctgg
      481 gggcggcagc catcgtgggc tctttcttca ctgccggggc atcaatggcg ttatggggtt
      541 cagccctggc agccggtggt ttttctgcca ccacaatgct gttttcactt ggagccagca
      601 tgattctggg cggtgtggcc cagatgctgg ccccgaaggc aaaaacaccg gattaccgcg
      661 caacggataa cggcagacag aacacgtact tttcctcgct ggataacatg attgcccagg
      721 ggaacccgat gccggtgcct tacggggaaa tgctggttgg ctcccgccgt atatcccagg
      781 acatcagcac ccgtgatgaa ggcgggggcg gaacggtcgt ggttgtcggg cgacagggat
      841 aaaacataaa aaaatcccgc agtgatcgcg gagctgcggg gacagacaaa tgaagatcaa
      901 tgttaaggag ttgtttttgt tactcgggca aaaaaacact aacgcagcga aattataagc
      961 gccacagtca gtgtgtgaaa atgtgaagat attcagaatt tttatgccat taccggtttt
     1021 aaccaacagg attatcggtg ggcatgaaag aaaaccccgg tatctgctga taccggggtt
     1081 tctctttagc atggcagaaa tgtgtttcat gcttttcggg cgaaggatat ccgacttctg
     1141 tacggaatgg caagtggcgg ttaatttatt caggggaagg ctgtatggga aaaggtggcg
     1201 gtaaggcaca cacgcctcgt gaggcgaagg ataatctcaa atccacgcag atgatgagtg
     1261 tgattgatgc gattggtgag ggaccgatag aaggtccggt gaagggactg cagagtattc
     1321 tggtgaacaa aaccccactg acggacacgg acggcaatcc cgtgatacac ggtgtgaccg
     1381 cggtctggcg tgccggggag caggagcaga caccaccgga aggctttgag tcctccggag
     1441 ctgaaaccgg actgggcgtg gaagtgacga aggcaaaacc ggtgacgcgc accattacgt
     1501 ccgcgaacat tgaccgcctg cgggttacct tcggggtgca gtcactggtg gagaccacct
     1561 caaagggtga ccgtaacccg gcatccgtcc gcctgctgat tcagttacag cgtaacggta
     1621 actgggtgac agaaaaggac gtcaccatta acggcaagac cacctcacag ttcctggcct
     1681 cggtgattct ggataatctg cctccccggc cctttaacat ccggatggtc agggagacgg
     1741 cggacagcac cacggaccag ctgcagaata agacgctgtg gtcgtcatac accgaaatca
     1801 tcgatgtgaa acagtgctac ccgaacacgg ccattgtggg gctgcaggtg gatgcggagc
     1861 agttcggcgg ccagcagatg acggtgaact accatatccg cggtcgcatc atccaggtgc
     1921 cgtcaaacta tgacccggaa aaacgcacgt acagtggtat ctgggacggc agtctgaaac
     1981 cggcatacag caacaacccg gcctggtgtc tgtgggacat gctgactcac ccgcgctacg
     2041 gcatgggaaa acgtctgggg gcggcggatg tggacaagtg ggcgctgtat gccatcgggc
     2101 agtactgcga ccagacggtc ccggatggtt tcggggggac cgagccgcgg atgaccttta
     2161 atgcgtacct ggcacaacag cgtaaggcgt gggacgttct cagtgatttc tgctctgcga
     2221 tgcgctgtat gccggtatgg aacggtcaga cgctgacgtt cgttcaggac cgcccgtcgg
     2281 atgtggtgtg gccgtacacc aacagcgatg tggtggtgga tgataacggc gtgggattcc
     2341 gctacagctt cagtgccctg aaggaccggc acacggcggt ggaggtgaat tacaccgacc
     2401 cgcagaacgg ctggcagacc tccacggaac tggtggaaga cccggaagcc atactgcgct
     2461 acggacgcaa cctgctgaag atggacgcgt tcggctgtac cagccgcggt caggcccacc
     2521 gtgccggact gtgggtgata aagaccgaac tgctggaaac gcagacggtg gatttcacgc
     2581 tcgggtctca ggggctgcgg cacacacccg gtgacattat tgaaatctgt gataatgact
     2641 atgccgggac cctgaccggc ggacgtgtcc tgtccattga tgctgccacc cgcaccctga
     2701 cgctggaccg tgaggttacc ctgccggaga caggtacatc ggcggtgaac ctgattaacg
     2761 gcagcggtaa gccggtgagt gtggacatca ccgcacaccc cgcgccggac cggatacagg
     2821 tcagtaccct gcctgatggt gtggagacat acggggtgtg gggactctcc ctgccgtcac
     2881 tgcgccgtcg cctgttccgc tgtgtctccg tccgggaaaa cacggacggc acctttgcca
     2941 tcacggcggt gcagcacgtg ccggaaaaag aagccatcgt ggataacggg gcgcactttg
     3001 acggcgacca gagcggcacg gtgaatggtg tcacgccgcc agcggtgcag cacctgaccg
     3061 ccgaagtcac cgcagacagc ggggaatatc aggtgctggc gcgctgggac acgccgaagg
     3121 tggtgaaggg cgtgagcttc ctgcttcgcc tgaccgtggc agcggatgac ggcagtgagc
     3181 ggctggtcag cacggccagg acgacagaaa ccacataccg cttcacgcaa ctggcgccgg
     3241 ggaactacag gttgacagtc cgggcggtaa atgcgtgggg acagcagggc gatccggcat
     3301 cggtatcgtt ccggattgcc gcaccggcag cgccgtctcg gattgagctg acaccgggct
     3361 attttcagat aaccgccacg ccgcatcttg cggtttatga tccgacggta caatttgagt
     3421 tctggttctc ggaaacgcgg attaccgata tcaggcaggt tgaaaccaca gcccgctatc
     3481 ttggtacggc gctgtactgg atagccgcca gtatcaatat caaaccgggc catgattatt
     3541 acttttatat ccgcagtgtg aacaccgttg gcaaatcggc attcgtggaa gccgttggtc
     3601 gggcgagcga tgatgtggaa ggttacctgg attttttcaa aggcaagata accgaatccc
     3661 atctcggcaa ggagctgctg gaaaaagtcg agctgacgga ggataacgcc agcagactgg
     3721 aggagttttc gaaagagtgg aaggacgcca acgataaatg gaatgccatg tgggctgtca
     3781 aaattgagca gaccgaagac ggtaagcatt atgtcgcggg gcttggcctc agcatggagg
     3841 atacagagga aggcaaactg agtcagtttc tggttgccgc caatcgtatc gcgtttattg
     3901 acccggcaaa cgggaatgaa acgccgatgt ttgtggcgca gggcaaccag atattcatga
     3961 acgacgtgtt cctgaagcgc ctgacggccc ccaccattac cagtggtgga aatccaccgg
     4021 cattttccct gacaccggac ggaaagctga ctgctaaaaa tgcggatatc agtggtaacg
     4081 tgaatgcgaa ctccgggacg ctcaacaacg tcacgattaa cgagaactgt cgggttctgg
     4141 gaaaactgtc cgcgaaccag attgaaggcg atctcgttaa aacagtgggc aaagctttcc
     4201 cccgggactc ccgtgcaccg gaacggtggc catcagggac cattaccgtc agggtttatg
     4261 acgatcagcc gtttgacc
//
LOCUS       DAZFJF010000243         4272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFJF010000243 DAZFJF010000000
VERSION     DAZFJF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.245"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..258
                     /locus_tag="ACHKAU_004988"
     CDS             <1..258
                     /locus_tag="ACHKAU_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system, LEE associated
                     protein"
                     /protein_id="HFO5533870.1"
                     /translation="GFSFFIEIIDSNVFITFALEYNENAFFSFFNALAPERTQGVIEH
                     IFVYDNKLCLSCLLTNIDVFSLMNTFQQHVQIIERVRRMTS"
     gene            255..2282
                     /gene="escV"
                     /locus_tag="ACHKAU_004989"
     CDS             255..2282
                     /gene="escV"
                     /locus_tag="ACHKAU_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO5533871.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            2266..3606
                     /gene="escN"
                     /locus_tag="ACHKAU_004990"
     CDS             2266..3606
                     /gene="escN"
                     /locus_tag="ACHKAU_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO5533872.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFASLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            3666..3986
                     /locus_tag="ACHKAU_004991"
     CDS             3666..3986
                     /locus_tag="ACHKAU_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO5533873.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKSIEKLSFAIKE
                     HYFD"
     gene            3979..>4272
                     /locus_tag="ACHKAU_004992"
     CDS             3979..>4272
                     /locus_tag="ACHKAU_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFO5533874.1"
                     /translation="MTSISLKRNLIEPVSFQPSDDWPQGEESFNDIFNRIKKRTPDTS
                     FIRMASLWKRLFYAPGQRRRYFEQGERSFSIICGKLRGIVLTIKCSNGTIYLSI"
BASE COUNT         1207 a          768 c          977 g         1320 t
ORIGIN      
        1 ggtttttctt tttttattga aataattgat agtaatgtgt ttattacgtt tgccttagag
       61 tataacgaaa atgcgttttt ttcttttttc aatgcccttg cccctgaacg gacgcaggga
      121 gtgattgaac atatttttgt ctacgataac aaattgtgct taagttgttt gttaaccaat
      181 attgatgtgt tttctctaat gaatactttt cagcagcatg tgcagattat tgaacgagtt
      241 cgcaggatga catcatgaat aaactcttaa atatatttaa aaaagcagag tcatatcacg
      301 atcttattct ggctctcttc ttctttatgg ctgtaatgat gatgattatt ccattgccaa
      361 cagttgtggt ggatatcatt atcgcgatta atatatcgac agcgttgctt ttattaatgc
      421 tctcaatcta tataaaaaat cctcttgagc taacttcttt tcctacaatc ctgttgatta
      481 cgacgttgat gcgcctgtcg ctcagtgtta gtacaaccag acttattctg ctgcatcacg
      541 atgcgggtga cattatttac tccttcggta actttgttgt tggcggtaat attgttgttg
      601 gcctggttat ttttaccatt atcaccattg tacaatttat ggttattaca aaaggggctg
      661 agcgtgttgc tgaagtgagt gctcgttttt cccttgatgg tatgccagga aaacaaatga
      721 gtattgatgg tgatatgcgc gcaggcgtta ttgatccgct tgaggcaaaa gttcttcgct
      781 cccgagtgca aaaggaaagc cagttttatg gttcaatgga cggggcgatg aagtttgtaa
      841 aaggggacgc tattgccggt atcatcattg ttttagttaa cctgtttggt ggcgtgctca
      901 ttggaatgtg gcaatttgac atgccattta gtgaagcact tagcctgttt tctgtattgt
      961 ctgtcggtga tgctttggtt gcccagatcc ctgcgcttat tatttccgtc actgcggggg
     1021 ttgttgttac tcgtgttccc ggtgaaagcg aaaaagaaga aaaccttgca ggtgatattg
     1081 ttcaacaggt ttctgttaat agtcgccctt ttttaatcag tgccgcactc atgatcgtaa
     1141 tggcgattat tcctggcttt cctacattgg tcttcttgtt tctggcggtt tgcctgttgg
     1201 gtatcgcctg gaaattacaa aagaaaagaa cgtttggaac cggaaataat aaggacgcta
     1261 tgggagctga tttgtctaat agtcaaaata tctcaccggg tgccgagccg ttgattttaa
     1321 acttaagtag taatatttat agctcagata ttacacagca gattgaggtt atgcgttgga
     1381 atttctttga ggaaagtgga attccattgc ctaagattat tgttaatcca gttaaaaata
     1441 atgatagcgc tatagaattt ttgctctatc aagagtcaat atacaaagat actcttacag
     1501 atgatactgt ttattttgag gctgggcatg cagagatatc atttgaattt gtccaggaaa
     1561 agctttcggc taactctatc gtgtataaaa cgaatgaggc taaccaacaa ctagctcacg
     1621 tctcaggtat ggatgtttat gcaaaaacaa atgacaagat aacgtttttg ctaaagaaac
     1681 ttgtattgtc taatgccaaa gagttcatcg gcgtacaaga aacgcgttat ctgatggaca
     1741 tcatggagag gaaatataac gaacttgtga aagagcttca gcgtcagctt ggtctgagca
     1801 agattgtgga tattcttcag cgtctcgtag aggaaaatat ctcaattaga gatctacgaa
     1861 ctatttttga aacgcttatt ttttggtcca caaaagaaaa agatgtggtt atcttgtgcg
     1921 aatacgttcg tatcgccctc cgtcggcata ttttaggtcg ctatagcgtt cgcggtacac
     1981 ttctgaatgt ctggcttatt ggctctgata ttgaaaatga gttacgagag tcgatcagac
     2041 aaacgtcatc aggttcgtat ttgaatatct caccggagcg aagtgagcag attattggct
     2101 tcttaaaaaa tattgtgaat ccaacgggga atggcgtcat tctgaccgct ttagatatca
     2161 ggcgctatgt gaagaaaatg attgaaggtt cgttcccgtc agtccccgtg ctctcttttc
     2221 aggaagttgg gaataatatc gaacttaaag tattaggaac ggtaaatgat tttagagcat
     2281 gattctgtat tggaaagata cccacgtatc cagaaagtgc tcaatagcac tgtgccgaca
     2341 ttatcattaa attcgtctac cagatatgaa ggcaagatta ccaatatcgg cgggacgatt
     2401 atcaaggcgc gtttaccaaa agcgcgtatt ggtgcctttt acaagataga acccagtcaa
     2461 cgtttagctg aggtgatcgc tattgatgaa gatgaggttt tcctgctgcc ttttgagcat
     2521 atttccggca tgtattgtgg ccagtggcta agttatcagg gggaagagtt caagattcgt
     2581 gttggggacg aactccttgg gcgactggtt gatggtatag gcagaccaat ggggagcaat
     2641 attactgcac cttatcttcc gtttgaacgc agtttgtatg ctgaaccccc cgatccttta
     2701 ttaaggcagg ttattgatca gccatttacg cttggcgtca gggcaataga tggtttactt
     2761 acttgcggta ttgggcaacg catcggtatt ttcgcggggt ccggcgttgg taaaagtacg
     2821 cttttgggaa tgatttgtaa tggcgcatcc gcggatatta ttgtccttgc ccttatcggc
     2881 gagcgtggtc gcgaagtaaa tgaattcctc gcgcttttac ctcaatccac gctttctaaa
     2941 tgtgtactgg tcgtcacaac gtcagaccgt cccgcgctgg aaagaatgaa ggccgcattt
     3001 acggcgacaa ccattgcaga gttcttccgg gatcagggca aaaatgtatt attaatgatg
     3061 gattccgtaa ctcgttatgc ccgagccgcg cgtgatgttg ggcttgcatc gggagagcct
     3121 gatgtaagag ggggattccc tccgagtgtt tttgcctcgt tacccaaatt attagagcgt
     3181 gcgggacctg cgccaaaagg ttcaataact gcgatttata cggtgctgtt agaaagcgat
     3241 aatgtgaatg atcccatcgg tgatgaagtt cgctctattc ttgatggaca tattgtgctt
     3301 acccgagaac ttgcagagga aaaccatttc cctgcaattg atattggttt aagcgccagt
     3361 cgtgttatgc ataacgttgt taaatcggaa catttacgtg cagcggctga atgcaaaaag
     3421 cttattgcaa cctataagaa tattgaactg cttattcgta ttggtgagta cacgatgggg
     3481 caagatcctg aagcggataa ggcaataaaa aacaggaaag caattcagag ctttatacaa
     3541 caaagcacca aagatatcag tagttacgaa aaaacgattg aaagcctatt caaagtggtt
     3601 gcctgaatat gttggacaga attttatcta ttcgtaaaag cagagcgaat cgattgagag
     3661 aatcaatggc taagattaac tctcagataa aagaggtgga tggtaaactc gatgactgcg
     3721 agcaagccat taaagagtcg attgcctcta agcaggcgta ttgtgcatcg ctggtgaatc
     3781 tggataaagt gtcgttgtat aaataccaga ttaaaaacaa tgcatttgat gagcaaaagc
     3841 agcgattata tgagaaaaaa tcctcacttt ctaaagaaaa gcgttcattg ctcgattcac
     3901 aaaaacgaac aaaagaggat ctacaacatg ttaataaatc aatagagaaa ttatcttttg
     3961 ccataaagga acattacttt gactagcatt tctctaaaaa gaaatttgat agagcctgtt
     4021 tcttttcagc cgtcagatga ttggccgcag ggagaagaaa gttttaatga tatttttaat
     4081 cgcataaaaa aaagaacacc tgacacttcg ttcatacgaa tggcttcttt atggaaacga
     4141 ttgttctacg ctcctggaca gaggcgacgc tactttgaac aaggagagcg ttcatttagt
     4201 attatttgtg gaaagttacg cggaattgtt ttaacaataa aatgtagtaa tggaaccatt
     4261 tatctgtcta tt
//
LOCUS       DAZFJF010000244         4226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFJF010000244 DAZFJF010000000
VERSION     DAZFJF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.246"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(202..1851)
                     /gene="pgi"
                     /locus_tag="ACHKAU_004993"
     CDS             complement(202..1851)
                     /gene="pgi"
                     /locus_tag="ACHKAU_004993"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO5533875.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
     regulatory      2088..2284
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2376..3725
                     /gene="lysC"
                     /locus_tag="ACHKAU_004994"
     CDS             2376..3725
                     /gene="lysC"
                     /locus_tag="ACHKAU_004994"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO5533876.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     VLATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3870..>4226
                     /locus_tag="ACHKAU_004995"
     CDS             3870..>4226
                     /locus_tag="ACHKAU_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HFO5533877.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGA"
BASE COUNT         1002 a         1064 c         1081 g         1079 t
ORIGIN      
        1 atttatctga taaaaaaatg ccgacataac gtcggcattg ttattaaggt tatttaatca
       61 atcgtgctat gtattaattg ccgaatacga tgtacatatc ggcatcgacc tgtaggcctg
      121 ataagacgcg accgcgtcgc atcaggcatc ggttgccgga tgcggcgtga acgccttatc
      181 cggcctacat atcgacgatg attaaccgcg ccacgcttta tagcggttaa tcagaccatt
      241 ggtcgaacta tcgtggctgc tgatttcttt gccatctttc agctctggca gaatacggtt
      301 cgccagctgt ttacccagct ccacgcccca ctggtcgaag gtgaagatgt tcagaatcac
      361 gccctgagta aagattttgt gctcatacag cgcaatcaac gcacccaggc tgaacggagt
      421 gatttcacgc agcaggatgg agttggtcgg gcggttacct tcgaacactt tgaacggcac
      481 cacgtagtca agcgttgccg gatctttacc ctgatcgcga tattcctgct caaccacttc
      541 gcgggattta ccaaacgcca gcgcttcggt ctgggcgaag aagttagaca gcagtttctg
      601 gtgatgatca gagagcgggt tatgggtgat agccggagcg atgaaatcgc acggtaccat
      661 tttggttccc tggtggatca gctggtagaa cgcgtgctga ccgttagtgc ctggttcacc
      721 ccagataatc gggccagtct ggtaatccac aacgttaccg ttacggtcaa catacttacc
      781 gttggactcc atattgccct gctggaagta cgccgcgaaa cggtgcatat actggtcata
      841 cggcagaatc gcctcagttt ccgcaccaaa gaaattgttg taccagatgc caaccagcgc
      901 cagcagtaca ggcaggtttt tctcggcagg cgtggtggag aaatgcttgt ccatcgcgtg
      961 tgcgccagaa agcagttcaa cgaagttatc aaagccgatg gagagaacaa tcgacaggcc
     1021 aatcgctgac cacaaagagt aacggccgcc gacccagtcc cagaactcga acatgttggc
     1081 agtatcaata ccaaactcgc caacggcttt ggcattggtt gagagcgccg caaagtgttt
     1141 cgcaacgtgt ttttcatcac ctgccgcttt caggaaccag tcacgcgcgc tatgggcgtt
     1201 ggtcatggtt tcctgagtgg tgaaggtttt agatgctacc aggaacagcg tggtttccgg
     1261 gtttactttt ttcagcactt cagcgatgtg agtcccatcg acgttagaaa caaagtgcat
     1321 gttcaggtga tttttgtacg gacgcagtgc ttcggtcacc atgtacgggc cgaggtcaga
     1381 accgccgatc ccgatgttca ctacgtcagt gattgctttg ccggtataac ccttccactc
     1441 accggagata atcgcttccg agaaggtttt catcttctcc agcaccgcgt tgacttccgg
     1501 cattacgtct ttgccatcaa ccaaaatcgg ggtattgcta cggttacgca gcgctacgtg
     1561 cagcacagcg cggttttcag tgcggttgat cttctcgcca gagaacatcg acttaatcgc
     1621 gcccgccaga tcgcactctt tcgccagatc ttgtaatttc gccagcgtct cttcagtgat
     1681 gcggtttttg gagtaatcca ccagcatctg atcgccgaag gttgcggaga acttagaaaa
     1741 acgatcgccg tctttagcaa aaagatcggc gatcgtaacg tctttcattt catcgaagtg
     1801 tttctgtagt gcctgccagg cagcggtctg cgttggattg atgtttttca ttagcaatac
     1861 tcttctgatt ttgagaattg tgactttgga agattgtagc gccagtcaca gaaaaatgtg
     1921 atggttttag tgccgttagc gtaatgttga gtgtaaaccc ttagcgcagt gaagcattta
     1981 ttagctgaac tactgaccgc caggagtgga tgaaaaatcc gcatgacccc gtcgttgaca
     2041 accgccccgc tcacccttta tttataaatg tactacctgc gctagcgcag gccagaagag
     2101 gcgcgttgcc caagtaacgg tgttggagga gccagtcctg tgataacacc tgagggggtg
     2161 catcgccgag gtgattgaac ggctggccac gttcatcatc ggctacaggg gctgaatccc
     2221 ctgggttgtc accagaagcg ttcgcagtcg ggcgtttcgc aagtggtgga gcacttctgg
     2281 gtgaaaatag tagcgaagta tcgctctgcg cccacccgtc ttccgctctt cccttgtgcc
     2341 aaggctgaaa atggatcccc tgacacgagg tagttatgtc tgaaattgtt gtctccaaat
     2401 ttggcggtac cagcgtagct gattttgacg ccatgaaccg cagcgctgat attgtgcttt
     2461 ctgatgccaa cgtgcgttta gttgtcctct cggcttctgc tggtatcact aatctgctgg
     2521 tcgctttagc tgaaggactg gaacctggcg agcgattcga aaaactcgac gctatccgca
     2581 acatccagtt tgccattctg gaacgtctgc gttacccgaa cgttatccgt gaagagattg
     2641 aacgtctgct ggagaacatt actgttctgg cagaagcggc ggtgctggca acgtctccgg
     2701 cgctgacaga tgagctggtc agccacggcg agctgatgtc gaccctgctg tttgttgaga
     2761 tcctgcgcga acgcgatgtt caggcacagt ggtttgatgt acgtaaagtg atgcgtacca
     2821 acgaccgatt tggtcgtgca gagccagata tagccgcgct ggcggaactg gccgcgctgc
     2881 agctgctccc acgtctcaat gaaggcttag tgatcaccca gggatttatc ggtagcgaaa
     2941 ataaaggtcg tacaacgacg cttggccgtg gaggcagcga ttatacggca gccttgctgg
     3001 cggaggcttt acacgcatct cgtgttgata tctggaccga cgtcccgggc atctacacca
     3061 ccgatccacg cgtagtttcc gcagcaaaac gcattgatga aatcgcgttt gccgaagcgg
     3121 cagagatggc aacttttggt gcaaaagtac tgcatccggc aacgttgcta cccgcagtac
     3181 gcagcgatat cccggtcttt gtcggctcca gcaaagaccc acgcgcaggt ggtacgctgg
     3241 tgtgcaataa aactgaaaat ccgccgctgt tccgcgcgct ggcgcttcgt cgcaatcaga
     3301 ctctgctcac tttgcacagc ctgaatatgc tgcattctcg cggcttcctc gcggaagttt
     3361 tcggcatcct cgcgcggcat aatatttcgg tagacttaat caccacgtca gaagtgagcg
     3421 tggcattaac ccttgatacc accggttcaa cctccactgg cgatacgttg ctgacgcaat
     3481 ctctgctgat ggagctttcc gcactgtgcc gggtggaagt agaagaaggt ctggcgctgg
     3541 tcgcgttgat tggcaatgac ctgtcaaaag cctgcggcgt tggcaaagag gtattcggcg
     3601 tactggaacc gttcaacatt cgcatgattt gttacggcgc atccagccat aacctgtgct
     3661 tcctggtgcc cggcgaagat gccgagcagg tggtgcagaa gctgcatttt aatttgtttg
     3721 agtaatattt ccctactggc gtataacgct caccgattgt tatacgccat cctcactgaa
     3781 aacatagatt aaataatatc tgaaaagcaa aatcactcct gtagaacagt attataatca
     3841 ccgaaaaata tcatttaaca ggagtgttaa tggctacttt aatggaaaaa gatgcacttt
     3901 taaacggagc cagtcagtgc atcgcgtttt tatcaaatat cattgataat tgctcagttt
     3961 cgtcgcatca ggattcaggc gatgctctca agcgattagt cagctatcgc gattatcttt
     4021 attctacccc cgctgaactt gttgatttta cacaaggtaa gattctcctt cagcaggtaa
     4081 gaactcaata tcagcatgaa ttcaacaata ctacgcattc agaaaataaa gcatcttttg
     4141 attctatttg gcagcgtctg acaaatcatg aagtcacacc gcaacagcat cccattggtt
     4201 ttgttttagg tgggcaaccc ggagca
//
LOCUS       DAZFJF010000245         4181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFJF010000245 DAZFJF010000000
VERSION     DAZFJF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.247"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..709
                     /gene="ydeO"
                     /locus_tag="ACHKAU_004996"
     CDS             <1..709
                     /gene="ydeO"
                     /locus_tag="ACHKAU_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO5533878.1"
                     /translation="LDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFTENTIKKYL
                     QCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAA
                     CKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTF
                     SQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRC
                     QSHTGMNTGNTMSALAI"
     gene            1111..2793
                     /gene="ydeN"
                     /locus_tag="ACHKAU_004997"
     CDS             1111..2793
                     /gene="ydeN"
                     /locus_tag="ACHKAU_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5533879.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2845..4002
                     /gene="ydeM"
                     /locus_tag="ACHKAU_004998"
     CDS             2845..4002
                     /gene="ydeM"
                     /locus_tag="ACHKAU_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5533880.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3968..4090)
                     /locus_tag="ACHKAU_004999"
     CDS             complement(3968..4090)
                     /locus_tag="ACHKAU_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533881.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
BASE COUNT         1278 a          879 c          829 g         1195 t
ORIGIN      
        1 tttagataaa gattacgcct tctctgacgg cgagatcctg atggtagata acgctgttcg
       61 tacgcatttt gaaccttatg agcggcattt taaagagatc ggatttactg aaaataccat
      121 taaaaaatat ctacaatgca ctaacatcca gacagtgacg gtgcctgttc ctgcgaagtt
      181 tttacgtgct tcaaatgtac cgactggatt gcttaatgaa atgattgctt atctcaactc
      241 ggaagaacgc aatcatcata atttttcaga acttttgctt ttttcttgcc tgtctatttt
      301 tgccgcatgc aaaggtttca ttacactatt aactaacggt gtgctatccg tttccggaaa
      361 agtgagaaat attgtcaaca tgaagctggc gcacccatgg aagctgaaag atatttgtga
      421 ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc aaacgacatt
      481 ctcacagatt ctattagatg ccagaatgca gcacgcaaaa aatttgatac gcgtagaagg
      541 ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt acatcttatt ttatttatgc
      601 gttccgcaaa catttcggca acagtccgaa gagagtttct aaggagtacc gttgtcaaag
      661 tcacacgggt atgaatacgg gcaacacgat gagtgcttta gctatttgat tatttgctaa
      721 cgagtagtca accacgcacg ctacgtaaga attatatggg gcagccattc cctgccccgc
      781 gttgttttta ggcgatatat ttattgaaat aaataagtga catccatcac atatttatgc
      841 acttgcataa cctgttgcat gattatttat gatctcaatt cggcattttg tcagtaaaat
      901 gcaataattt attaaatatc aataaattag ttgtttatcg gcgagaaatc acttaataga
      961 acagaaagta atgtcaacgc tttatggact gttatttccc tttttttagc taaatctgct
     1021 atctctttat gtgactaact tcacttacat ccacttattt ctcttcgtaa aattacttcg
     1081 gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa aagtgtcgta
     1141 agtacctcga tatctttgat actggcatct ggtatggctg catttgctgc tcatgcggca
     1201 gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt tctcagactt tacgccgact
     1261 gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga tcttggttat
     1321 ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa tcgtgaagtt
     1381 gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa atcaacgccg
     1441 acgctccttt cattaatgga tgaaggcgta cggtttacta acggctatgt ggcacacggt
     1501 gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg ctttggtgtc
     1561 tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt gcctgaatta
     1621 ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc aaaaatcagt
     1681 aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt caccacattt
     1741 tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg attccacgct
     1801 gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg tgtccccgca
     1861 aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga tcgtgccaaa
     1921 acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct gccaaatgat
     1981 aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac agcagataac
     2041 tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga acaactgaag
     2101 aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg tgcggttatc
     2161 gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac ctatcctggc
     2221 ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg taattatgac
     2281 aagctgattt ccgcaatgga tttctacccg acagctcttg atgcagccga tatcagcatt
     2341 ccaaaagacc ttaagctgga tggcgtttcc ttgctgccct ggttgcaaga taagaaacaa
     2401 ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt tgacgaggaa
     2461 aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga cgattacccg
     2521 cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa taacgattat
     2581 tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac ggatctacag
     2641 caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca aggcgtggta
     2701 agagagttta tcgacagcag ccagccaccg cttagcgaag taaatcagga gaagtttaac
     2761 aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc ggaattttcc
     2821 gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag ttttcaatgt
     2881 aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac tcatgaaaaa
     2941 tggatggatg acagcactct gaaagagttc atcaaacaat atatcgcagc gtctggcaat
     3001 caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga ttttttccgt
     3061 aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa tgcattacaa
     3121 acgaatggca ttttattgaa taatgaatgg tgtgcttttc tcaaagaaca tgagtttctg
     3181 gttggtatct cgatcgatgg cccccaggag ctacatgacc gttacagacg cagtaattca
     3241 ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc atatcaagtt
     3301 gagtttaata cgttaaccgt cattaataac gttaatgtcc attaccctct tgaggtttat
     3361 cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct cgaaaccggg
     3421 acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat tgatttttct
     3481 gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg ggttaaaaac
     3541 gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt tttggggaat
     3601 gggcatacca gttgtatttt ccaggagtcc tgcaaggata atctggttgt tgaaagtaat
     3661 ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg aaacattaat
     3721 aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa acggatttca
     3781 gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc taagcatcgt
     3841 attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa aaaccttttt
     3901 tcaaccatgg taccttatat gaacgccatg gtggagttag ctaagaacag agtaccgctt
     3961 tatcacatta tggatgttgc aaaacaaatg gagaataatt aataacacgc ccttttagct
     4021 ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt cctgtcgggt
     4081 gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg cgtcagggca
     4141 ttagcaatac ccttgattat caatagcata accataacta a
//
LOCUS       DAZFJF010000246         4082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFJF010000246 DAZFJF010000000
VERSION     DAZFJF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.248"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..67)
                     /locus_tag="ACHKAU_005000"
     CDS             complement(<1..67)
                     /locus_tag="ACHKAU_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5533882.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
     gene            complement(64..639)
                     /locus_tag="ACHKAU_005001"
     CDS             complement(64..639)
                     /locus_tag="ACHKAU_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5533883.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(654..1007)
                     /locus_tag="ACHKAU_005002"
     CDS             complement(654..1007)
                     /locus_tag="ACHKAU_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5533884.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKAU_005003"
     CDS             complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKAU_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5533885.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1426..2310)
                     /locus_tag="ACHKAU_005004"
     CDS             complement(1426..2310)
                     /locus_tag="ACHKAU_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5533886.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVASSRYPKHWLTVGDPAREFTMTQS
                     APLMVLPDPDEFVVVQVK"
     gene            complement(2368..2715)
                     /locus_tag="ACHKAU_005005"
     CDS             complement(2368..2715)
                     /locus_tag="ACHKAU_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5533887.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(2752..>4082)
                     /locus_tag="ACHKAU_005006"
     CDS             complement(2752..>4082)
                     /locus_tag="ACHKAU_005006"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5533888.1"
                     /translation="EYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGY
                     DGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTAC
                     SAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQ
                     FEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLAD
                     ELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLAT
                     MLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPEM
                     TVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAA
                     GSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          864 a         1290 c          981 g          947 t
ORIGIN      
        1 gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac tgcagcacgg atatcagtat
       61 gtttcatcgc tttatcacaa tccttagttg ttgtttcaac gcatagccca gttcttttgg
      121 cagacgttct cgccggatac ggttaacgtt ctcatcaaaa gcctgcttca gtggggccgc
      181 catcggaatt ttaaccacct gaatgggaag acgattacgc tttttccttc ctttatcgtc
      241 attgcccttc gcataccggg cttctggcaa acgttgcata acatgccagc gcccattatt
      301 taatcgctgg atgaatgccc gctgataacg atgctgaccg gctttaagta tgctgttcgg
      361 acgatgacca agcatcctga tccccagctt aatagcaggg agatcaccgc ggttaacgat
      421 aattctggca ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag
      481 tttccggctc accctggtct cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt
      541 agccacgcgg ttaatagcca ttgctgaagc cgacggaatg gcgtttttac gaacccggct
      601 cagattttca atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt
      661 cgccggttaa ctgcgggtgg ttgcccacgg ttcagccaga gataacaact gcccccgtca
      721 tccggagaaa cacgatccac ccagaacgtc tcaccattaa tggtcaacgt gtcaccacgc
      781 cgcacggcac gaaccgtatc cgtccgcaca aataatgacg ggctgcttcc ttcaatacgg
      841 atcccactac tggcaaaccc cagcgactcc ggatcgtcaa aaaccccctg aacttcgccg
      901 ccacgctgtg caccggaggt gaactgcgca ctgatgccca tcacttcaac aatcgtactg
      961 tccaccccag caagggcggc atcaaaggca ttctgaaaat cacgcataaa cagccattcc
     1021 accatcaacg tgtgtttttg catctgagga cataatcaga atcacccgac caacatccgc
     1081 aagctcaacg gattcccccg tttcaccatc aacgccacag agatggaggc aggtcagaac
     1141 tctgatgcgc gttaacgcgc cggatgtttc ctcacgaaca tcatgagccg cgttttccct
     1201 ctcccggata tccatattca taacctgtac atcatcgccg gatgactgca tttcctcttc
     1261 ccattctgcc acccgctgcg ctatctctgc ggcactcccg gatatatccg gctcacgccc
     1321 cagaatcagg gccagttcat caagccgttt cagattttgc tctttcgttg ccatatcatc
     1381 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgtac
     1441 caccacaaac tcatccgggt ccggcaacac catcagcggc gcggactgcg tcatggtaaa
     1501 ttcacgggcg ggatccccta ccgtcagcca gtgtttcgga taacgggaag aggccaccac
     1561 aatggccaga tcgccgtaat accctttgaa ggacaccacc gcgcccaggt ctttcaccgc
     1621 tgtttcgagt tgcgaattag agccgcgacg ggtatccagt ttttcgcgga acaatttaaa
     1681 gccattcagc agacgccaga cggtaccgtc cataatggca atattcacaa gaccgctggc
     1741 ctggtcgcag tagaggtcaa gatcatgtgt aggatcgaac gtgtcacgat cctgttttga
     1801 ccactcctta ccactaccct gagtgatgtt attcttcgtc gacctgccaa aatcgacctc
     1861 aattttctcg aactggtctc cttccatcgt gtatttgcca tacagcacgg cattcaccgc
     1921 ctgcatttct tccacccgga caatggcgtg ctcttcctgt ttgaggttat cagtgatgat
     1981 acgcagacga cggtaggccg ggtcgttcag ctgagccgga tcttcaccgg gaagacgctc
     2041 aaccgcctgc tggtaattaa attcgtgttt cggcttgacg tagcccggac gtaacacgcg
     2101 ggtttcacca ccacgatgac gcagcacttt tccttcaaca accggggaaa cataggccgc
     2161 caccggcgtt tttccggtaa ttttgtccag catcacctct tcggtatgga aattcaccgt
     2221 acggcggaaa aacagctcca gaaacagcgc acgaaatttc actttttgtt cggtataacc
     2281 gagtaactgg cgggtcgtaa acaatcccat aaatcagttc ctttcattca gaaatcagtc
     2341 aggccaccgc ggtggcctga taacgtgtta cggcagcgcc gcgtgactca gggcactgcc
     2401 ggcaaaggcg ttggcctttt tgtgttcatc cacactttca ggccagcgga ttgcctccgt
     2461 cgcaaaggtc cccgacttgt aataggtcag cgccgtctct gtgccttcaa gcggcagtac
     2521 cagtatgcca accgcactac cggctttctg tccgtcccag accaccagtt tcccggtggc
     2581 ctcatccagc atcaggggcg tcagtgccgg tgttgccgag gaaatcccgc tgctgcctgt
     2641 ggcggtatga gccggatcat taccggcaaa aatacgtact tccacacgct gttcagtgat
     2701 agttttcgtc accatattgt aaaaacctcc tgttgatggt cagcactggc ttcatggcat
     2761 ggccatgagc attttcacgt ccgcatcacc gtctgctgac gtctgtggca cgccaccctg
     2821 taccgctgcc ggtgaatggt tcgccatgat gcgttcaaac atggcggttg tggatgcaga
     2881 gaccggttct gccttacctg atcccgcagc cagcacagcc cgggcgctct ccacagtcat
     2941 tcccgggcag gcagccagct gttcagcctg cgcctcagcc ccttttgcct catccagtgc
     3001 catgatctga tcacggagtg agggtccggc atccgcctgc ggtgaagcag ccaggatcgg
     3061 gcgggctttt tccaccgtca tctccggcat cgccgccagc gttgccgcca gttgttcacg
     3121 acctttcgct tcttcacacg ccataatgcg atcggcttca ctctgcgcgg atgccaccgg
     3181 ctgctgtggt gccgccgcgg ccagaatcgc ccgggcctgt tcaacgctca tgccctgttg
     3241 tcctgccagc atcgtggcaa gctgttcacg tcctttcgct tcctggcatg tcaggatccc
     3301 catcactcgc tggttctcct gcgcggcggc ttccgttgca gttaattgcg gcatagtgcc
     3361 tcctctgaca ttactgttca gcgccgtggc catcacactg atggcatccg acgcattgat
     3421 taattcatcc gccagcccgg cctcaatgcc ggactgacct tcaaaaacgg cggcctctgt
     3481 tcccgtgaca gcatcaacag acagaccggt atacatcgct actttttcag caaacatccg
     3541 gcgcgccgca tcaatgcgct gttgcatgtc ctggcgaacc tctgccggca acgcttcaaa
     3601 ctgattgcca tccaccttgt gcgcccctga gtaaatcagc gtgatatcaa caccggcctg
     3661 cgccagatga ccggcatagc tgacatggct catcatcacg ccaatggagc cgatacggga
     3721 tgtctgggta accagccgtc gggagcaggc cgacgccagc agcatggctg cagagcaggc
     3781 cgtgtcattg cacagtgccc agaccggctt ctgctgacgg aggcggtaaa tcatgtcagc
     3841 gcagtcaaac gcgccggcgg cctgcccgcc cggactgtca atgtccagca gtacgccccg
     3901 cacctggcta tccgccattg cctgctgaag acaggcgaca atgccgtcat agcctgtcat
     3961 tccggaaaat ggccgcatac cccccagccg gtgcaccagc gtgccggtca ccggcagtac
     4021 agcaataccg ttcaccaccc ggtaaacacg ggccggtcgt ttacctccgg ccatgtactc
     4081 gt
//
LOCUS       DAZFJF010000247         4052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFJF010000247 DAZFJF010000000
VERSION     DAZFJF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.249"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(31..729)
                     /locus_tag="ACHKAU_005007"
     CDS             complement(31..729)
                     /locus_tag="ACHKAU_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5533889.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(729..1058)
                     /locus_tag="ACHKAU_005008"
     CDS             complement(729..1058)
                     /locus_tag="ACHKAU_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5533890.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            complement(1055..3634)
                     /locus_tag="ACHKAU_005009"
     CDS             complement(1055..3634)
                     /locus_tag="ACHKAU_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5533891.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANGKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEHQKRLNELAQ
                     QAARFEQQQGAKQAAISAQARGLTDRQVQRESEEQRLRDVYGDNPDALAKATSALKNT
                     WSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQS
                     WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAIQAAAANFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGISFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDEIQAQMRDGGVFSG
                     GRR"
     gene            complement(3615..>4052)
                     /locus_tag="ACHKAU_005010"
     CDS             complement(3615..>4052)
                     /locus_tag="ACHKAU_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5533892.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLD
                     MHFSGLMYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERLDEDKMLMQKAAGLAGGVR
                     FGGDGGGDILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
BASE COUNT          799 a         1386 c          975 g          892 t
ORIGIN      
        1 gaatcgctga ttctgtctgt gtcattggat ttactgcgaa agtttgttaa tggaaaggaa
       61 accgccaaaa ttagccacca tgccgcgcat ctcacacccg cgcatgcact tgctgcatct
      121 gtccttacgg atatcggtgg tgggtttatc gaactcatcc gccaccgcag gaccgttata
      181 cccgcattca tctccccggt aatcccacat acaggtgttc gccagcatga tgcgaccggg
      241 aaacagcgcc ccatccgtct cggtcggtgt agccagcaca aacgaggccg tcatggctgt
      301 cagctgcgac atctgctcca ccacccagcg gtcactcagc tcctgctccg ggtccgcctc
      361 cggattgccc gcaacgaaat tcaccgcatc cagaaaacgc gcatacaccc ggcggcggac
      421 caccgtggcc cccaccaggc tctgcaggtc ctccgccatt ccggtgacca gaccgaacag
      481 attcgacacc gtcagcgacg ggcgggcact gctgcccttc ccgttcatct caaagccact
      541 gccgtcaatc gggtatgcct gatattgccg cccctgccag gtaaccgcct cccctttttc
      601 attcagctca ttgcagaaaa aataccgctc accaccctgt accgtcaggt cgatttccca
      661 gagtaccacc cgcggtgact gctctgattt aaccgactcg ttcagacttt cttcgtgaat
      721 atcctgcatc agttcaccac ctgcttaaac tccgcgctga attcaacccg cagcatgcga
      781 acccgtgatg accaggcggc acaggtcacc tttatctgcc gccaggcata aggcggtgtc
      841 cacagaaacg ccttccagcc accgtgctct gccaggaatg cctccagatg tcgggcctcc
      901 tcccgggtca cggaaagcgt cacacggtat gttttcaggt cagcattcag ccccgccgcc
      961 atacgctgcg aatacccgtc accaaaacgc acttcacgca ccgatggctg cgagttcacc
     1021 tccatatccg gcttcacttt ccagcgaaat gttttcatcg cctgcctccg gaaaagacgc
     1081 cgccatcacg catctgcgcc tgaatctcat cctgcgcccc cttgcgggcc atgtcataca
     1141 ccgccttcat cagctgcggc cccgcctgtc cgttggtgcc gtcgttctga atcaccacgt
     1201 gattgttctg attaaaacta atgccttccg cccgccgcat ctgcgccgga cttccggcac
     1261 cgcccacata accaccttcc gcatacccgc gcatcagacg atacaggttg ccgacgccaa
     1321 tccggctggt tgcctccttc gtgaagacaa actccccgcg gtggacaata ccggcaggtt
     1381 cgtatttgcc accggttccc gtaaatcctc cggtcgcgaa atggaagttc gccgccgcag
     1441 cctgaatggc cgtccccgtg gaggcagacg ccccaccacc gaaagcacca cccatggcgc
     1501 tgccaatact cccgacaatc cccaccatgg cctgcttcag aaaaatctct gtcagcatgg
     1561 agagcacaga acgggtgaaa ccacgccagc tctgttcgct gccggtcagc atcgctgcca
     1621 tattctgtgc aataccgtca aaggtctgcg tggccacgct tttaacctgc gaaaaactgt
     1681 ccgtcgcact ttctgcccac tcgccccagc cggacttcag accggccatc cagcttccac
     1741 gaagctgctc ctccgcagac caggtgttct tcagtgcaga tgtggccttc gccagcgcat
     1801 ccggattatc accgtacacg tcacgaaggc gctgctcttc cgactcccgc tgcacctgac
     1861 ggtcggtgag gccccgcgcc tgggcactga ttgccgcctg cttcgcgccc tgctgctgct
     1921 caaaccgcgc agcctgctgt gccagctcat tcagccgttt ctggtgttca actttgtctc
     1981 ccagctcagc cagctggcgt ttgtactcca gcgtctcttt ctcatgggtc agcagggatt
     2041 tttcctgctc agataactgc cgtttcgtgg ctgcctcttt caggaccgca tactgatttt
     2101 ccgctttcca taaatcgcga cgctgctggc tgattttccc attcgcaccg ctgtgttttt
     2161 ccagcgtccg gagctcggtt tcaagcgcca gcagggcagc atgcgcctgg tcttcctgac
     2221 gctcaccggc tgacactttg actcctgacg acttcggctt tttcagcgtc gattcataat
     2281 cctttttcgc cgccgccatc agcgtgttgt aatccgcctg caggattttc ccgtctttca
     2341 gggccttatt cagttcttcc tgacgggcgg tatatttctc cagcggcgtc agcagacgct
     2401 catacgcctt ctgcgcctct ccggtatact tcagctgtga tgcgtcccgt tcggcccggt
     2461 ccctggcggc cagttcaccg gctttttcca tatccgactg cagcgtggcc gctgccagcc
     2521 ccagacgggc attttcccgg tcattccatg cgccctgaag gttggcccgg aaagaggagg
     2581 ttttaccgcg gcgctggctc cgactctggt accactgcca ttttttatcc gcctcatcaa
     2641 atgccttctg cgcactggcg agcatatccg ctgaggattc cggacgaccg atatccagaa
     2701 tggcatccca catcgatttg aatgccttcc ctgttttatc cgcccaggtc tccagtgttc
     2761 ccatgttttc tttcaggcga cgggtctgct catcaaagcc tttcgtggcg atatcgttcg
     2821 ccgcctgcaa tgccccggcc tcgtctccgg aacgctgcag ctgtgcaaca tacgcaatct
     2881 gctctgccgt cacgttacgg aactggcgcg ccatcgccat cagtcccgac gtcgggtcag
     2941 tggtcagctt cccgaaggct tcagcgactt tatccacctc cacaccggat gcagacgcaa
     3001 aacgcgcgac actctggttg atggcatcaa actgttcacc accacgcaca ccggcattca
     3061 ccagggctgc cagtgactct ctcgcctggt taaacgtcag ccctgctgcc tgcccggctc
     3121 ttgagagagt cagcatacga tcggcagtca gtccggactg attaccggaa agaaccaggg
     3181 ttttattaaa cgctgaaagc gtggaatccc cctggtacca ggcgtacgcc agcgcacctg
     3241 tcgccaccgc cagcgaggtg accccgacca tcggcagggt gatcgcaccg gcaagccccc
     3301 tgagcatggg gatcatcccg ccgaaggagt ccttcacctg accgccctgt tgcagcagga
     3361 tcagccaggg attctgacca ccggcaagct gcgtggcgat atccgtaaac tgtgcgggca
     3421 gggtgcgcat ggccgcttta tactgtccga cggaaatccc ggctttttgc gcagccagcg
     3481 cctgacggct caggccctgt tcaacagcag tggcggtttt tctggcgtca gtatccagtc
     3541 ctgaaaaatg acgccttacc cggctcatct gctcatcgaa acggaccgca tccagactca
     3601 ggtcaataat aagatcacca accggctggg acatatctca cacctcccgg aatccccgct
     3661 gaagccatca ttaatgcggc atcatccacc atgacatccg ccacatccgc agacgataaa
     3721 atatcgcccc ctccgtcccc accgaaccgg acgcctccgg caagtcctgc cgctttctgc
     3781 atcagcattt tgtcctcatc cagcctctcc acctgctctt cctcatgccg ggggacaagc
     3841 agactgaaat cagagggatg catatccgga tcgcaaaaaa acaggctgag tacagcgtac
     3901 atcagcccgg aaaaatgcat atccagttgg gtatcctgaa aataatgcgt gcggtaaaaa
     3961 tgtcgccagt cggcatattc ggtggatgtc atcccggcaa gcatggcgcg ccagtcaggc
     4021 ctccccatct ctcgcgccag tctgagggca aa
//
LOCUS       DAZFJF010000248         4029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFJF010000248 DAZFJF010000000
VERSION     DAZFJF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.250"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(258..536)
                     /locus_tag="ACHKAU_005011"
     CDS             complement(258..536)
                     /locus_tag="ACHKAU_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO5533893.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
     gene            964..1110
                     /locus_tag="ACHKAU_005012"
     CDS             964..1110
                     /locus_tag="ACHKAU_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533894.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
     gene            complement(1247..1894)
                     /gene="nleG"
                     /locus_tag="ACHKAU_005013"
     CDS             complement(1247..1894)
                     /gene="nleG"
                     /locus_tag="ACHKAU_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO5533895.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
     gene            2078..2668
                     /gene="espM1"
                     /locus_tag="ACHKAU_005014"
     CDS             2078..2668
                     /gene="espM1"
                     /locus_tag="ACHKAU_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO5533896.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(2887..3943)
                     /locus_tag="ACHKAU_005015"
                     /pseudo
     CDS             complement(2887..3943)
                     /locus_tag="ACHKAU_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1154 a          772 c          881 g         1222 t
ORIGIN      
        1 ataaaaagtt gtttttcgtg tctttaagtg ataccagatg gtattgcgct aaatgtacca
       61 atgggtatac caagagcact aaaaacaaag gggcaaccac tttgtaaccc cttgattatt
      121 tgcagaagcg cagaaaatcg aactctggaa ccctttcggg gcatcctttt tcaagaatga
      181 agaaaaaaat aactaaaaaa tcaagttgtt aggaagttat tcatggctag tgtaccaact
      241 cttttctggg ctaatagcta tgatttgttt gtattatttg tctctgtctc tattattttt
      301 gacatccatg cgacttctga tattgataat ctcagttgtg gagttctgtc tgcgcttccg
      361 ggaggacgca tatcaaaact acaggatctt ctgttaaaca ctgtaaagcc atctacattg
      421 catgttgttg agtctaagat acaattttta gtagatgatt tatctgctat aggcctagat
      481 atttcaggtt gatgccaagt gtgtcctgaa aaaatacttt ttattgaaaa tggcattttt
      541 atcctcacat tatgtctttc tcttatagtg caatttaatg tatttagaaa cattaaacta
      601 tgaatctaaa aaggctacgc agagtactgt tacactttaa caaatcatat ttaccactct
      661 ggaaatggga tggctggttc ataaaataat gagtcttttt caacggttgt tacattggtg
      721 tactgatgat tatgccttgt cgctaagagc taccataacc gtacagcgtg attcatccat
      781 ggcgaataaa tcgactgata aatccccctg agtttcagct cttgctgttg gaccttgccc
      841 gtttcgctat ggaacgcgta ccagctagag aatattattc gttctcctcc aatgttacgc
      901 aagttttgta aacggtaacc aatcgtgatt agtcctgcat gtaacggaca tcaagctgtc
      961 tggttgattt ttattgccag cagttcgaga gagcagacaa ctgcgtccga tggaaaaatg
     1021 ggcacgacag ggtattcagg caacctcgct gaggaggctt atggtacaga ttgtaatgtg
     1081 aagtataaaa cgttaaaaat tccattttag aaactaaata gctgtaatat aagttcgatc
     1141 taatctacta ttttgacaga cagattgtat caagaattag ggagtatgaa atagatggca
     1201 ataactagta aaattaattt tgcaacattt aaatcgaacc atacagtcaa attctagtgc
     1261 atatattttg tgtggctaat atgcaaaaat attgttctat tagattaaaa tggcattcat
     1321 ctttgctgac aatcatttcc ggaacaaagg gttcacggct gagagggtgt ggagcgttgc
     1381 gtcggataag ttcagtgagt gcattgtggt catataatga acatatttct gagttttttg
     1441 catttctgac aaaaacacca gtttcaggaa tgcataatgt tatcgggcaa ttcaggtgtt
     1501 gttcatgaca ggaaaagtct tgatgactaa cagtaaatgc atgagagtta attttgtttt
     1561 gtattgcact actgacgttt tggcgcgtac atggggttgc acggctctgt tccatatata
     1621 aacggaatgc ctgcaaaaaa gtatggcctc tgttaagctg tatctccaga ttttctgcga
     1681 gaaggcgatg ccctcttgca ctaaggcgat ccaacagtcc gccggaaacc ggttccacgc
     1741 taaaactgtc cataaatgaa atatgatgaa gtctcatagt gcggcctctc acaacaagct
     1801 gttcactctg actaactctt ccgacatgac gtaaagcctc cagttcactt tcagacaata
     1861 ctctttcact cgaaaagttt aatatgacag gcatattttc tccctcttta gtctatgtta
     1921 ttgaaccata ttggtgacag gagtcaagat actaaatatg tgataggatt caaacaaaaa
     1981 attaaatttt gaacgaatgc tatttacaca tgtatttttt taattaaaat gattatatac
     2041 aatttttgta agcaaatttt aatgaaaagg atttgttatg ccagtaaatg cgacaggtgt
     2101 gtccttcagc tcttttggta tcagttatca taaagataat tctttccggg gcaccatccg
     2161 tgggaagaat gatgaggtcg tgaagtgttc aatgggagag cgctctatcc gctttaatgt
     2221 taacaaattt agcggctgta tactggagac ggtaagcagg cagagtacta aagatattca
     2281 tggatgggta agtgatgaac ggacagtata tccgtcaagg gttatcaacc aagagattga
     2341 taattgctgc cttcaaaaaa atgcaaaaat ttcttctgaa gagaggaaaa tggtcttttc
     2401 tcttgtaagt aaggagtttg aactaactct tgatgttaaa gcggcacaaa gttctattaa
     2461 tcatataata ataggaaatg cttcttttgg caaaaaaatg gatgctcttt gcgatggtat
     2521 gagccgagct gtaaaaaaca gtacaacaga ttacatagca aacgtgcttg cagacaagtt
     2581 ttatcagaaa catatcgccc cgggtgttga tattgtaaaa ctacgaaatg aaatcccagg
     2641 ttatatgagt cgtgttatac aggggtaagg ttggaggagg ctatgcgcca gtatcattgg
     2701 ttttcatcct gtaaaaagat atgagtaaaa tatacaacag gcgttacact atatgtatgt
     2761 atccaaataa ctcatagttc acctgtacag ttataaatcg taaagaaaat tgcagcgcgt
     2821 cgtacgcaga aacgtgctgt gattgactat ttatagcttt gtcacaacga caagattcag
     2881 tagtgtttac acgggcggga gaaatgacgg aataactaaa tagctgcgcc taatatcgca
     2941 catttttgcc agcccatgtt tgccttcggg gaatggactg acggattcca taaaatggcg
     3001 aactttttca atagttgcca cattgagcgg cactgatgat tacgcgttat tgtggcgtga
     3061 agtatctgcc atagctattc aaaatgattc acccatggag attaaaccgg ctgataaatg
     3121 actctgcact tcggtttatc cttcagccag cgctgggttt cccggccttt gtggataatg
     3181 tagttgtcct cgagcagcgt gatggttttc gtccgacagt atgtagcttt aagccgcttc
     3241 agcagtctga tgaacagcgc cgaacttttg ctgttggcgc ccacacagct gacttaacct
     3301 gtcccgctgt gcagcgctac gtccagagaa tatttttcat tttgttccgg cgtgacccct
     3361 cgtttttgct tcccgagcag tttccagtcc gcaccgattt tgggattaag atggataaat
     3421 acttcatctt cataaaagac cggatgctct gtgctgcatt cgtccagtgc gttatggatt
     3481 gttgccatct ttttatcttt aggcggggca cggatacgta gagttggcgc agcccttcgc
     3541 cacacaagcc ccacagacga caaccagcgg cgaatggttc cagcatgtaa caggcagccg
     3601 gttatctcat tgatttttat tgtcagcagt tctgtactcc agcgtgaata attgtagcca
     3661 aagtcgcgag tgaatatttt acctgttcac gtaatagtgt gtagatatgc gcaaacggcc
     3721 agcggcgggc tcgctcggca ggtaatgatt tcagtccctc aacacccgac tgtgtgaact
     3781 agttaatcca gcgcccaaca gaggaacggg cgcagcatag tgtgcgggca acgtcgctga
     3841 cacggtcgcc ccggtgcagc atcagcatgg ctgtcagcct tttatggatg gctttctgta
     3901 ttaggcgtcg ttcgtcacgg gaaattggtg gtatgatcga cattgctcag tccggttggt
     3961 gatttgtatt gatttggcga ttgatcagat cgcacaatcg ggttgagttc cctctaaatg
     4021 atctactat
//
LOCUS       DAZFJF010000249         3949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFJF010000249 DAZFJF010000000
VERSION     DAZFJF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.251"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            220..1257
                     /locus_tag="ACHKAU_005016"
     CDS             220..1257
                     /locus_tag="ACHKAU_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5533897.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            1276..2232
                     /locus_tag="ACHKAU_005017"
     CDS             1276..2232
                     /locus_tag="ACHKAU_005017"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO5533898.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            2323..2811
                     /locus_tag="ACHKAU_005018"
     CDS             2323..2811
                     /locus_tag="ACHKAU_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFO5533899.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            2894..>3949
                     /locus_tag="ACHKAU_005019"
     CDS             2894..>3949
                     /locus_tag="ACHKAU_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5533900.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLY"
BASE COUNT         1296 a          699 c          812 g         1142 t
ORIGIN      
        1 aagcattcac gccattatca acacaaatca aggccaatca actgtcaaat aagattttaa
       61 tgatgaataa ctgcaaatag tgatgcatat ctcatttgca gtaatttgtt gaggaaaaaa
      121 atattttatt gccgcagagt gtttaggagt acatgaggta cgactatatg gaataacaga
      181 aatcatatct taaattgata ttttggaagg aactcattta tggttttaat aaaaaagaat
      241 gtcttatcgg cttcaattgc aacagtcctt ttaggctcaa caggagcctt tgcggcaaac
      301 gatcgcggct ttacctccga cactgactgg tcagaagaac aagcgggctt taatggacat
      361 gttggtacta catttgaata tgagaccaaa agcactgata actatttagg taaaacagta
      421 aaggaatata ctaaaacaca cgagattttc caggcctatt tcagccaacc taattggcaa
      481 tttgcagcaa tctatggcct taagtctatc gatcgccggc aagaagaaaa aggctatcac
      541 gaaaatgaaa caagtctgca aaactatata tcactaaaca aaacgttcac tattggtaac
      601 ggtttcgact tcagtctggt atacgacctg atgtataccg acggaaatat agatagccct
      661 tatgttacag gcttgcatac tgtaactgtt gacaacagtt ttcgccctac gttgacgtac
      721 tttaactctg actggaacgc aggtttttat tcaaacgttg aatacttata cagtcgaaat
      781 gataagagct cctggggcgt acgcgaagaa gaaggtcaaa gtatactatt tcagccctat
      841 aaacgttttg gtgcatggga gtttggtatt gaatttttct accagaaaaa gcataacgat
      901 gaatttaatc acggtaaaat aaatgatcgt agtatttata ccgaaactta ctacgagcca
      961 attatcaaat atgcttttga aaacgcaggt aatctgtatc tacgcacccg cttcggacac
     1021 gggaaaacag aaaacgcaga tagtttgcaa tacaatgcaa acagaaccta tttcaaaaca
     1081 attcgaaaag ccactctagg ttatgaacaa aaaataggtg acgactggat agccaagatt
     1141 gaatataaac atgcctggga aaaagagcac aaaaactttt atgatccaag agatgaagaa
     1201 aaatataatg tgctcaatca ggataatatt tatgttcatg ctttctatcg cttttgatta
     1261 aacagaggga aacctatgtt cagcgcacta gatattaaaa cgttgatgca aggaacttta
     1321 tatggtgatc catcactcag ggttgatacc attcggccaa tccatagtcc tttagttggt
     1381 ggactctcga ttgttatgac acctggagat ttgttgcata taccaacgac tggtgcggat
     1441 ataattatcg gaccggaaga gatcattagt tctaatgcta aggcaaagat tgtagtcgat
     1501 tacctgaacg tgaataatct taacaaagtt ttaagatatt ttaaggtaca taagtatcgt
     1561 ttgtttgagc aagaaaacac ttcaacgata cctgatgtat acattggtaa acactgccag
     1621 ataggtatga actgccattt tatgcctggt gttaaaatta tgaactgtgt gaccataggt
     1681 gataatgttg ccattcatgc gaatacagtg attaaagaag gtactattat tggcaatgac
     1741 gttatcatag attctaataa ctccatcggt aattatagtt ttgaatatat gtcggatgaa
     1801 cgtgattcat atgtccgagt tgatagcatc ggtagagtaa tcataggtga tgatgttgaa
     1861 atcggttgta acaacaccat tgaccgaggg acacttggag atacgattat aggccaaggc
     1921 actcgtattg ataatcaagt gcagattgga catgactgca taatcggtaa taaatgcctg
     1981 attgtttcac aatgcggttt ttcaggacac gtagttttag gcgaccatgt tattacacat
     2041 ggtcaagtcg gaatcgccgg tcacatatca attggtagtt attcggtaat taaggctaaa
     2101 tcaggtgtaa gccattcttg tcctgaaaag agtgaccttt ttggttaccc tgccaaaaat
     2161 acgcgagaat acaacaaaaa tctcgctgtc cttaataaac taactaaaca gcatggtgta
     2221 tataaacagt gagtcgttga gaagtattat ttatatacat taacattaac actgacatag
     2281 aaatagctta actaagctat actactttta ttgagagttt aaatgaaaaa taaattgctt
     2341 attgcggctg tgttgactgg tcttctttca ggatgcgttg atggcaattc accctctggc
     2401 agtcaggaag gtgtcgtgaa agcggatcct cgctattcag aatgtaacct ccctgatgta
     2461 aatgaaagag ggccaattgc tgcgggtctt tacatttccg gaaccctccc ggaaggctat
     2521 tggttaatga agccagagcg taaaatgcat tataagggag atggtattta tcagcttatt
     2581 tattctgaaa aagcaggtgc ggtaacaatg caatttgcga ctatgaactg gaacccgcag
     2641 ttcactgtaa caggtcttga attgccatta aatcaggcta aaacactcaa gaaaggaggc
     2701 atggataaaa atacaaaagt cgttattcct gccgatggaa agtatgtttg gacgattcaa
     2761 attggtcctg ataaaaaggc aatagccgcc atggtagcac catgtaaata gttattttta
     2821 gcactccact attcaacatt cgttgaatag tggagtatca gtattccaaa attcttgtta
     2881 atgagagcag attatggccg cggtaattct acgcaaagtt gaaaaaattt atgacaatgg
     2941 attcaaagct gttcacggca ttgatctcga tataaacgat ggtgaattca tggtttttgt
     3001 tggaccatct gggtgtgcca aatcaactac attacgcatg attgcaggtc ttgaagacgt
     3061 atcaggtggt gaaatttaca ttagcgaccg ccttgttaat acccttccgc ctaaagatcg
     3121 cagcattgca atggtgttcc agaactatgc gctgtatccc cataagactg tatttgacaa
     3181 tatggctttt ggtttaaaaa tgcaaaaaag gccaaaagaa gagattaaga aaattgtcga
     3241 agaagttgca gaaaaacttg aaattagcga tttactttac cgaaaaccga aagaaatgtc
     3301 gggtggtcag cgtcagcgtg tcgcggtagg ccgtgcaatt gtccgtaagc cagatgtttt
     3361 cttatttgat gagccactat ctaaccttga tgcaaaactt cgtgtttcaa tgcgagtcaa
     3421 aattgctcag ttgcatcagt ctctcaagga tgagggtcat ccagcaacca tgatttatgt
     3481 tacacacgat caaattgaag cattgaccct cggtgatcgt atttgtgtgc ttaatcaggg
     3541 gcgaataatg caggtcgaca caccagttaa tctgtataac caccctgcta acagattcgt
     3601 tgctggcttt attggttccc cgtcaatgaa tttgatagat gcggttgtac gtgaggaaga
     3661 cggtaaactc tatattgaca ttgccaaaaa tattcggttg tacatacctg agaataaaca
     3721 atcagtactc aaagcgtata tagataaacc cgtttgtttt ggtattcgtc cagaacatat
     3781 ctctttgagt ttgaattgca aagaaatgaa tacagtcact ggtgcattaa gtattattga
     3841 aaatatgggc agtgaaaaat atctttactt taccgttaac aacaaagagt ttattgcaaa
     3901 agtcaacgat caatcaatta ctacagccga tattggtaaa aatttgtat
//
LOCUS       DAZFJF010000250         3919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFJF010000250 DAZFJF010000000
VERSION     DAZFJF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.252"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..83
                     /locus_tag="ACHKAU_005020"
                     /pseudo
     CDS             3..83
                     /locus_tag="ACHKAU_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            87..278
                     /locus_tag="ACHKAU_005021"
                     /pseudo
     CDS             87..278
                     /locus_tag="ACHKAU_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(286..639)
                     /gene="cmi"
                     /locus_tag="ACHKAU_005022"
     CDS             complement(286..639)
                     /gene="cmi"
                     /locus_tag="ACHKAU_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO5533901.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
     gene            complement(812..1594)
                     /locus_tag="ACHKAU_005023"
     CDS             complement(812..1594)
                     /locus_tag="ACHKAU_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5533902.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(1596..2009)
                     /locus_tag="ACHKAU_005024"
     CDS             complement(1596..2009)
                     /locus_tag="ACHKAU_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533903.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2569..2799
                     /gene="vapB"
                     /locus_tag="ACHKAU_005025"
     CDS             2569..2799
                     /gene="vapB"
                     /locus_tag="ACHKAU_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO5533904.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            2796..3212
                     /locus_tag="ACHKAU_005026"
     CDS             2796..3212
                     /locus_tag="ACHKAU_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO5533905.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(3374..>3919)
                     /locus_tag="ACHKAU_005027"
     CDS             complement(3374..>3919)
                     /locus_tag="ACHKAU_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533906.1"
                     /translation="MQICKSITASADCEEIIKLIYLRRYFEIVDERGDAYQLLSNLFH
                     RRVAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALVSSPDEIKNL
                     YRRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRFDLIPEYVIM
                     ECDKLIALPPAANQSSVARIA"
BASE COUNT          988 a         1005 c          966 g          960 t
ORIGIN      
        1 gtattaaatt acttgataaa gttattatct atcaacaaca gaatgagcaa ttatatccag
       61 cacaggaaca tcttctttta caatgttcat atgatgataa atacgatttt aacgccagca
      121 ctcaccgtgg catcatcgga gagtcgctca caaggctcgg ggcgatgttt tctggtaaag
      181 agtaccagat actgcttcct ggtgaaattc acattaaaga aagtggtaag cgagaatata
      241 agggccggga ttccggcctt tttatgcagc ctttataaca gtgtattagt cattcgcata
      301 ttcaacatat ccctcctcac aatggtatat gccataatac attctcttac cctccgggcc
      361 ttcagcttta aatgggacaa gaagtgtttt ttcatcttca acatctgtta aacttctctc
      421 aatcttttcc catgtaattt tcgggatttc tgtaccaaga agttgaacat cgttgtccca
      481 gcgtttcatt ctttgcaggt aatcattttt tacaaacgtt ctggcctgtt catcgctgac
      541 ctgatagcat gccggacctt tatcttcaga ccagaaaaaa acagcagcaa gagcaataat
      601 cgttaaaata aaaataaatt tcatgctaat tactttcata aaatctccac aacttctgtc
      661 gttcattcga tataaaaaaa cattacgaat atatccgtac aatgtgagca tactgtgaag
      721 tggctttaac aattatgaaa taatacaaga gatcacatat tgcagtggtc tcttttttat
      781 agaagaaatc acaattcata attgtgactt attatgcatg tttgtttttc agcattgtga
      841 ccgcatcgga ctcaggcatc gaaaactgca cccggtgccg ggcagcgaca tccagcgcaa
      901 acaccttcgt gtagacctcc gttgagctga tggacttatg ccccatcaga ctctgcagga
      961 ccttcagcgg tataccggca tacagcatat gcatcgcata ggaatggcgg aacgtatgtg
     1021 gcgtgaccgg gacagagaac gtcacaccat cagcggcagc ggcggcaacc gcctccccaa
     1081 tccaggtcct gaccgttctg tccgtcactt cccagatccg cgctttctct gtcctgcctg
     1141 ttcgtttatt acgccgttcc atggggattt tcaatgtggc caccatcgtc tgcagctggc
     1201 tgacgtacca ggagtcggag agcggaacca gccggtgagt ctgctgcccg gcgggcgttc
     1261 ttcctgccgt cctggcggct ttttcggtcc gttgtttcag ggtcgcaagc tgcacaaacg
     1321 gatacggagg cgtgagcgaa aaatcccccc gcgtcagcgc cagtgcttca ttaatgcgcg
     1381 caccggtgtt ccacagtgtg gccagcagca tcttgcggcg cagatccggg acgtaatgga
     1441 gcagggcgct cacttcaggg gccagcaggt atttgggcag ttcatcatga accatcgaca
     1501 tctggcggag tgccagcgct gccggataat cgatggcaac aggaagctga gcggcatccc
     1561 gggcatggtg gatcggtgcc ggcaggtgct gcatatcagt gttcactcca cggggaaacg
     1621 tcgggcaggt tttccacaac ccggtacttt cccggataac ggagctggtt accgccacgc
     1681 gtccagacgg tgacaacaaa accccctccc tggttgtttt cggggagcag gcgatcgcac
     1741 tgctcactca gcatgtggat cacgaacggc gcatcgctgc cgtcttcaaa tatcagttcc
     1801 agcgcatcgc ggccatgcag cgttccctta ctgatgatga cgtactcagc cccccgcatc
     1861 tccctgagta aatgttttgc cgcatccggg acaagcagcc tggctgcgcc ggcattccag
     1921 ctgaggtaga catatccggc ctgcgcctgt acagactgcc agtaatccgt tctggcgaca
     1981 gcctgacctt cattctcaat aaaaaacatg ggtgactcct tttttctttt ccgggcaggg
     2041 acggtcatgc gataacgtca tccatcatca ctgctcttac cgtcacgcca tgtgataaca
     2101 tcatgccgtt atcatcatca ccatgataac aagcatccat ataaccacgc tgttaaatta
     2161 ccggaggacc atttccgaca gatccgattt ttctgttatt gacgggtttt cggaagtgta
     2221 tcacgctgag gaaataatca tatcgctgtt ttccgcccct gcgcgacgca tcacaggtca
     2281 gggagatgac acaacatata gtgtcatcag cagatgaatc cggcaccaca tgttgtgatg
     2341 acgggaggtg gcgttgtgtg gggggagagg aagatcctga acaacctttt ttcggatctc
     2401 cgataaaaaa gaaagaaata gagatatgct aaacatatgc ctggcatcta catatgctac
     2461 cgctatacat atgccacaca tctgcctata ctaaacatat gcacacaccc ggtgctttga
     2521 atattccgga tatgtttcac atatccatat gctcgggaga gaaagaacat gagaaccgta
     2581 tcgatattta aaaatggcaa caaccgcgcc atccgtctgc ctcgtgatct ggattttgag
     2641 ggggtgagcg agctcgagat cgtccgggaa ggggacagca tcatcctgcg ccccgtccgg
     2701 ccaacctggg gatcattcct agagtacgaa aaagccgatc cagattttat ggcggagcgc
     2761 gaggatgttg tcagcgacga agggcgattt aacctgtgaa caagacttat atgcttgata
     2821 cctgtatctg ctcgttcatc atgcgtgaac agccagaagc cgtcctgaag cgtctggagc
     2881 aggcggtgct acgcggccac cgcatagtgg tctcggccat cacctactct gaaatgcgct
     2941 tcggggctac cggcccaaag gcctcaccac gtcacgtcca actggtcgat gaattctgcg
     3001 cccgcctcga tgccatcctg ccctgggatc gcgccgcagt agacgccacc acgaagatta
     3061 aagtggcact gcggctggcc gggacgccga tcggcccgaa cgacacggcg attgccgggc
     3121 acgccatcgc cgccggggcg atactggtaa cgaataatac aagagaattt gagcgggtgc
     3181 ctgacctggt gctggaagac tgggtgaagt aagacactct tttcaaagtc aggagataag
     3241 ggcaccaaga aacagcccgt atacgggctg ttttaacaga tatctacagt aagcggtcct
     3301 aaactgtagg gtatcttcga agatcagact atcagggagc taaagcctat cttccggcgg
     3361 caaaaacagt ggctcaagcg atacgggcaa ccgagctctg gtttgctgcc ggtggcaggg
     3421 cgatgagctt gtcacattcc ataatcacgt actccgggat gagatcgaat ctggacggat
     3481 ctagttgaca gatgaactca ttttcaatgt ggtacgtttc gttaacgaat tttcgtatta
     3541 ccggatggtc ctgatccagt tccagtaaac ggaatacctg cagtttttca tagccgttac
     3601 gacagcgacg gtacaggttt tttatttcat ctgggctgct gacgagagcc tgaagtcgcg
     3661 ggtaatcaaa actgtccaca tactcccgga tatcccgcaa ggcctgctga attttctcag
     3721 gagccatttt ggggtagccc gagcccgcag cggcaggctc gcgataatcc agcggggcga
     3781 cgcgtcgatg gaatagattg gatagaagct gataagcatc accacgttcg tcaacgattt
     3841 caaagtagcg acgaagatag atgagcttaa tgatctcctc acagtctgct gacgcagtga
     3901 tggatttgca gatctgcat
//
LOCUS       DAZFJF010000251         3878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFJF010000251 DAZFJF010000000
VERSION     DAZFJF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.253"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..535
                     /locus_tag="ACHKAU_005028"
     CDS             <1..535
                     /locus_tag="ACHKAU_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5533907.1"
                     /translation="SLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDL
                     TVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDL
                     LKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKD
                     ATPGNVYAQADYKIAYE"
     gene            595..3099
                     /locus_tag="ACHKAU_005029"
     CDS             595..3099
                     /locus_tag="ACHKAU_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5533908.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3115..3864
                     /locus_tag="ACHKAU_005030"
     CDS             3115..3864
                     /locus_tag="ACHKAU_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5533909.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
BASE COUNT         1207 a          817 c          795 g         1059 t
ORIGIN      
        1 gtcacttctt gttgctggcg tagcaatgat tctttccaat aacgtatttg ctgacgaagg
       61 tcacggcatt gttaaattta aaggtgaagt tatctctgct ccatgttcaa ttaaaccagg
      121 tgacgaagac ttaactgtaa atctgggtga agtagcagat accgttctga aaagcgacca
      181 gaaatctttg gctgagcctt tcactattca cctgcaagac tgcatgttga gccagggtgg
      241 aaccacttat tcaaaagcga aggttacttt caccacagcg aatactatga cgggtcagac
      301 tgacctgctg aaaaacacca aagaaactga aatcggtggt gccacaggcg ttggcgtacg
      361 tattctggat agccagagcg gcgaagtgac tttaggtact ccggtagtaa ttacattcaa
      421 caatactaac agctatcagg aactgaactt caaagctcgt atggaatccc caagcaaaga
      481 tgctaccccg ggcaacgttt acgctcaggc tgactataag attgcttacg agtaatcaca
      541 ttaggccggg ttcgcccggc cacatcatta acggattaat gataagtgga acagatgtat
      601 aaaaaattaa aattaaccac aataagcgaa ttgattaaaa atatttattg ttcattatcc
      661 gttctcatca ttggttgtgc gtcagcttat gccgttgaat tcaacaaaga tttaatcgaa
      721 gccgaagatc gtgaaaacgt taacctttcc caatttgaaa ctgatggcca attacccgta
      781 ggtaaatact cattaaacgc tcttattaat aataaaagga cgccaatcca tctggacctg
      841 caatgggtat taattgataa ccaaactgca gtttgtctga ctccagagca attaacatta
      901 ttaggcttta ctgatgagat tattgaagag gctcagcaaa acctgatcga tggttgttac
      961 cctatcgaaa aagaaaaaca aattacaact tatctcgata aagggaaaat gcaattatcc
     1021 atatctgcac ctcaggcatg gttaaaatac aaagatgcaa actggacgcc tcctgaactt
     1081 tgggatcatg gtattgctgg ggcatttctt gactacaatt tatatgcctc tcattatgca
     1141 ccacatcagg gcgataattc acaaaatata agttcctatg ggcaggctgg ggttaatctt
     1201 ggggcctggc gcctgcgtac tgattaccag tacgatcagt catttaacaa tggcaaaagc
     1261 caggcgaaca acctggattt tccgcgtatt tatttgtttc gcccaatccc agcaattaat
     1321 gcaaaactaa ctataggtca atacgatact gaatcctcta ttttcgactc tttccatttt
     1381 tctggcgttt cgttgaaaag cgatgagaat atgttgccgc ctgatctacg tggttacgca
     1441 ccgcaaatca cgggtgttgc acaaacgaat gcaaaggtca ctgtctcaca gaacaaccgt
     1501 attatttatc aagaaaatgt tcctcctggc ccatttgcta ttactaattt attcaataca
     1561 ttacaggggc aactggacgt caaggttgaa gaagaggacg ggcaagtcac gcaatggcaa
     1621 gttgcatcta atagtattcc ttatctgacg cgtaaagggc agattcgcta caccactgct
     1681 atgggtaaac cgaccagcgt tggtggtgat tccttacaac aacccttctt ctggactggt
     1741 gaattctcat ggggttggct gaacaatgta tccctgtatg gtggttcagt tttaacaaac
     1801 cgtgattatc aatctctggc tgccggcgtt ggctttaacc ttaactcatt gggttcatta
     1861 tcttttgatg tcacacgatc tgatgctcag ttgcataatc aggataaaga aacgggttat
     1921 agctaccgcg ctaactattc aaaacgtttt gaatctaccg gtagccagct cactttcgct
     1981 ggttaccgtt tctctgataa aaactttgtg acaatgaatg aatatatcaa tgacactaac
     2041 cattacacga attatcagaa tgaaaaagag agttatattg tcacgtttaa ccagtatctt
     2101 gaatcattaa ggttaaatac atacgtaagt ttggctcgta atacttactg ggacgccagc
     2161 agtaatgtga attattcatt atcacttagc cgcgattttg atatcgggcc attaaaaaac
     2221 gtctccactt ctctaacatt tagccgaata aactgggaag aagacaacca ggatcaactg
     2281 tacctaaata tttcgattcc ctggggaacc agtagaacat tgagctatgg tatgcaacga
     2341 aatcaggata acaagatttc gcatactgct tcgtggtatg actcttccga tcgaaataat
     2401 tcctggagcg tttctgcttc aggcgacaat gatgaattca aagatatgaa agcgtcacta
     2461 cgcgccagtt atcagcataa taccgagaac ggtcgtctct atctctccgg gacatcacag
     2521 cgagacagtt attattctct gaatgccagt tggaatggtt cattcacggc gactcgtcac
     2581 ggagccgctt tccatgacta tagtggtagt gctgactcgc gttttatgat cgacgcagat
     2641 ggtgcagaag atattccgtt gaacaataaa cgcgcggtaa ctaatcggta tggcatcgga
     2701 gttattccat cagtcagcag ttacataaca acatcattaa gcgttgatac ccgaaatctt
     2761 ccagaaaatg tggatatcga aaactcggtt atcaccacca ccttaaccga aggtgctatt
     2821 ggctacgcca aacttgatac ccgcaagggc tatcaaatca tgggggttat tcgcctggca
     2881 gatggtagtc acccaccact ggggattagc gtaaaagata aaaccagcca taaagaatta
     2941 ggactggttg ctgatggcgg ctttgtatac ctcaacggca ttcaggatga cagcaaactt
     3001 actttacgct ggggtgataa atcctgtttt attcaaccac ccaatagcag caacttaacc
     3061 accggaacgg ttattttacc gtgtattagc caaaattaat tcaggatgaa actaatgcgc
     3121 tacttaaaca caaagaatct cattgcagca ggagtattac tcgcctgcat gagcagcatt
     3181 gcctggggtg ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc agataaaggg
     3241 gaagcattaa aacttactaa tcaaagcaaa aatctaccct atctggcgca aacatggata
     3301 gaagatacga aaggcaataa atcacgtgac tttattgtca cggttccccc tatggtacgt
     3361 ttaaatccca gtgagcaaat tcaaataaga atgataagtc aggaaaaaat tgctcaactg
     3421 cctaatgaca gagaaacgct cttctatttt aacgtgcgag aaataccgcc aaaaacagat
     3481 aaaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa
     3541 gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga
     3601 aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc
     3661 ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc
     3721 catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat
     3781 tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt
     3841 tccgtagcca aacgagataa ataatatgcg ctacttat
//
LOCUS       DAZFJF010000252         3821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFJF010000252 DAZFJF010000000
VERSION     DAZFJF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.254"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..232)
                     /locus_tag="ACHKAU_005031"
     CDS             complement(<1..232)
                     /locus_tag="ACHKAU_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5533910.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALD"
     gene            complement(371..604)
                     /locus_tag="ACHKAU_005032"
     CDS             complement(371..604)
                     /locus_tag="ACHKAU_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5533911.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALAKTSVGYLDQIAYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            718..1422
                     /locus_tag="ACHKAU_005033"
     CDS             718..1422
                     /locus_tag="ACHKAU_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5533912.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            1559..1846
                     /locus_tag="ACHKAU_005034"
     CDS             1559..1846
                     /locus_tag="ACHKAU_005034"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO5533913.1"
                     /translation="MKVLNVEKLHSFSRKHNQAKGALDSWYDEVIRENWKTTQDIRNR
                     FNSADFLPNNRVIFNIKGNNYRLVVQVVYQAGMVIVERVGTHAEYDKWRLK"
     gene            1843..2499
                     /locus_tag="ACHKAU_005035"
     CDS             1843..2499
                     /locus_tag="ACHKAU_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
                     /protein_id="HFO5533914.1"
                     /translation="MNRTSWRIIKNSEEHAAAMERLIELASSDLQPGTEDFDEFELLG
                     LLIEHYESREFPMDKPDPIEAIKFRMDQQGLSYADMKQYIGSASKVSEVLNRKRPLSL
                     SMIRRLHDGLGIPADILIQDMSAIEWSLVDAEEEETAMTSVIARCESAVTSPSAYFAE
                     KATESYFSKMLFSAVRGNGKCKEKRNVFSLISNLSSSFTAASNLNDEMTSDGNYLLLP
                     "
     gene            2496..2900
                     /locus_tag="ACHKAU_005036"
     CDS             2496..2900
                     /locus_tag="ACHKAU_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533915.1"
                     /translation="MKIELISKKVERLVMTRLEGDSTAKKAIKTTVNLNNELYTNVKD
                     SKLFRVRYFASVTIEGRLEMDITYDFDFRSEDDFSHEMAKSYEVRSIAPNMAYPYIKT
                     YAEQIILMSNLGRFTLPYFDFLANPMETNSNK"
     gene            3723..3791
                     /locus_tag="ACHKAU_005037"
                     /pseudo
     CDS             3723..3791
                     /locus_tag="ACHKAU_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
BASE COUNT         1187 a          783 c          851 g         1000 t
ORIGIN      
        1 catcaagcgc atacttaaaa gccctactaa tcggactgat gtctgatgcc attccgaaag
       61 cacacaagac cgaagcaata aatctccagt ccgttctgct tatcttcgat tcatgacagc
      121 caatcatctt tgccagaccg cgctgggtaa gcgttgacag gttgatgagt aaatcagttt
      181 cagcgcgatc aatttctcgc tgtgatagtt tgctgtaact tgtttgttcc atttcttaag
      241 atttccaata gtgaatagtt agttgaaagg tatgcgtgga aacgcatgtg gccttagttg
      301 gtcagatata ttgggactcg ctttgtcagc gacgtaggac gaatgtccat tgtgaaaata
      361 gcggtgttac ttatgcagcc gatgctctac gcgatacgaa cactaggttt tcctttttca
      421 caggtttata acccgtgaaa ttacgagtag cttcttcgat tgcattcgct ttatcagggg
      481 aagctcttcg aaatccatat gcaatctggt caagatagcc aactgaagtt ttcgctaatg
      541 cggcgagtcg cttccattcc tcactagaag cctcttttcg ccagcgtagt agttcattac
      601 tcattagtgc ctccgtttat cacacagaac aactttacca ttttgataaa tcatccgcaa
      661 tgtaaattta tcatattgcg tatttatcca tttgctaaat agagggaaaa ttgtgagatg
      721 gaaaacaaag atattcgcaa atcgaatctg gcgtttttgc tagatgagca taaaaaaatc
      781 gcgggtaaca ctaatgcaag ctttgccgat aagcttgggg ttagcccttc tcaactcacg
      841 caagtctccg gtgaaaaaag cactcgaaac ataggggata aactagcaag aaaatttgaa
      901 gccgcgcttg ggttacctaa tgggtggctt gatttggtac atgatgtaac accaattgca
      961 tcatgctcag attctttaac ttttgtcggt caggtaagaa aagggttagt gcgcgtggtt
     1021 ggtgaggcaa ttcttggtgt tgatggtgcc atcgagatga ccgaagagcg cgatgggtgg
     1081 ctcaaaattt atagcgatga tccagatgcc tttggtcttc gtgtgaaagg agacagcatg
     1141 tggcccagaa taaaatcagg agaatatgta ctcattgagc ctaacaccaa agtattcccg
     1201 ggtgatgagg tgtttgtcag aactgttgaa ggacacaaca tgatcaaagt tcttggctat
     1261 gacagagacg gagaatacca atttacaagc atcaaccagg accacaggcc aataacgttg
     1321 ccttatcatc aagtagcaaa ggtggagtat gtggctggta ttctgaagca atctcgccat
     1381 ctggatgaca tcgaggcaag ggagtggctg aaaagttcgt gacttcatcg tcacatagct
     1441 ggtaaccagt ggcctgaaga gacgtttggg tgatgtacat agcatttctg gataaaaata
     1501 cagattccct ttatgggaaa tgaatctata attcccaaag agggaacaaa atcggattat
     1561 gaaggtctta aacgtagaga agcttcacag ttttagccgg aagcacaatc aggccaaggg
     1621 ggctttagac tcttggtatg atgaagtgat aagagaaaac tggaaaacga ctcaagacat
     1681 acggaataga tttaattctg ccgacttcct tcctaacaac agggtaattt ttaatataaa
     1741 aggcaataac tatcggctcg ttgtccaagt tgtttaccag gcaggaatgg tcatagttga
     1801 aagagttgga actcatgcag agtacgacaa gtggaggctt aaatgaatcg aactagctgg
     1861 cgcatcatta aaaatagtga agagcatgct gcagctatgg aaaggctcat tgaacttgcg
     1921 tctagtgatt tacaacctgg aactgaagat tttgatgagt ttgaactact aggcttgctt
     1981 atcgagcact atgagtcacg cgagttccct atggacaagc cagatcccat agaagcaatc
     2041 aagttccgta tggatcaaca aggcctctct tatgccgata tgaaacaata cattggctca
     2101 gcatctaaag tatctgaggt cttaaatcgt aagcgtccat taagtctttc aatgatccgt
     2161 agactacatg acggacttgg aattcctgca gatatcttaa ttcaagatat gagcgcaatt
     2221 gaatggagcc tagttgacgc agaggaagaa gaaacagcca tgactagcgt cattgctcgg
     2281 tgtgagtcag ccgtcacatc accttctgct tatttcgctg aaaaggctac agaatcttac
     2341 ttttcaaaaa tgttgttcag cgcagtaagg ggtaatggca aatgcaaaga aaaacggaat
     2401 gttttttctt tgataagtaa cttgtcatca agtttcacag cggctagcaa cctgaatgac
     2461 gaaatgactt ctgacggaaa ttacttatta ttaccatgaa aattgaactc attagcaaaa
     2521 aagttgaacg cttggttatg acacggctag aaggcgattc aacagcaaaa aaagccataa
     2581 aaacaaccgt taatctaaat aatgaacttt acactaatgt gaaggattca aagctattta
     2641 gagtgagata ttttgcctcc gtaactattg aaggcaggct tgagatggac atcacatacg
     2701 actttgactt ccggtcagaa gatgattttt ctcatgaaat ggcaaaatca tatgaggtaa
     2761 ggtccattgc tcccaatatg gcatatccct atataaaaac atatgccgag caaattatcc
     2821 tcatgtcaaa ccttggtagg ttcactctgc cttattttga tttcttggcc aatcccatgg
     2881 aaacgaacag taataagtga aattccccac ccggcctcag cgccgggttt tctttgcctc
     2941 acgatcccca cacctaaaaa cacataacca attgtatttg ttgaaaaata aatagataca
     3001 acttgctaaa caacgcaatc cagatctccc tcaaatctct ttatttatcc tgtcgaattc
     3061 ctacaacaaa ataaaacacc ataagaatca atacgatatt tgaaaaccaa gagaatttat
     3121 cattttgcta ttgccattaa tttatcactc cgataaagtt cacccatcag caggacgcat
     3181 tactcaccag ggcggtgaat atacaacgat tcgaatatga atctacggcg ctgacaaagc
     3241 gcaataacca aagtgaactt tggggtgtgg tgaagggttc atggacggga atatgtcgca
     3301 cgtaaagcgg cgaggcctgc gggactattg ccgaattgaa gtaggccgaa acaggtcgaa
     3361 atgggtctcc cacctaccac accaccaaag ttcatcagga ggtctatatg acacgcagaa
     3421 ctcagttcaa aggcaattca cgttctcgtc gtcgtgagcg tttaaaggca aaggcattag
     3481 ctaacggcgt actggcccgc gaagaagcaa taagttcaga agtattacac cgccctactc
     3541 taagcagagc gcagattcag gctaaaggta ctcacgaaac gcctgagcgc atagaagacg
     3601 ctaagccaat taagttcatg gcacaggacg tgatctggca acagaaagaa tacagacgca
     3661 atctggagcg agcggccatt gtgtacgcga atgagtttgg acataagcaa ccagaaactg
     3721 gtgtatgtct tccaaacgta gccatttacg cggcaggcta ccggaaatca aaacaactga
     3781 cggcgaggtg acttgtgttg gtcgccagaa aatgaaatta g
//
LOCUS       DAZFJF010000253         3715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFJF010000253 DAZFJF010000000
VERSION     DAZFJF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.255"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..256
                     /locus_tag="ACHKAU_005038"
     CDS             <1..256
                     /locus_tag="ACHKAU_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5533916.1"
                     /translation="DSNHFSCPESFLTCPITLDTPETGVFMRNSRGAEICSLYDKDAL
                     VQLVETGGAHPLSREPITESMIMRKDECHFDTKREAFCCK"
     gene            338..979
                     /locus_tag="ACHKAU_005039"
     CDS             338..979
                     /locus_tag="ACHKAU_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5533917.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            1010..1132
                     /locus_tag="ACHKAU_005040"
                     /pseudo
     CDS             1010..1132
                     /locus_tag="ACHKAU_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(1920..2354)
                     /gene="uspF"
                     /locus_tag="ACHKAU_005041"
     CDS             complement(1920..2354)
                     /gene="uspF"
                     /locus_tag="ACHKAU_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO5533918.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(2495..3628)
                     /gene="ompN"
                     /locus_tag="ACHKAU_005042"
     CDS             complement(2495..3628)
                     /gene="ompN"
                     /locus_tag="ACHKAU_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO5533919.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGNVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1073 a          715 c          839 g         1088 t
ORIGIN      
        1 tgattctaat cacttttctt gcccggagtc atttttgaca tgcccgataa cgctggacac
       61 acctgagact ggggtgttca tgagaaactc acgaggtgct gagatatgct ctctatatga
      121 taaggacgcg ttagtgcaac ttgttgaaac tggtggagct catcctctga gtcgagaacc
      181 tataacagaa tcaatgatta tgagaaaaga tgaatgtcac tttgatacaa aaagagaagc
      241 tttttgttgt aagtgataat taatgatata aatatagttt tatgtacttt tattttttac
      301 taaagcaaaa ttaagttatt aaaatggagg gagaggaatg cctgtagatt taacgcctta
      361 tattttacct ggggttagtt ttttgtctga cattcctcaa gaaaccttgt ctgagatacg
      421 taatcagact attcgtggag aagctcaaat aagactgggt gagttgatgg tgtcaatacg
      481 acctatgcag gtaaatggat attttatggg aagtcttaac caggatggtt tatcgaatga
      541 taatatccag attggccttc aatatataga acatattgaa cgtacactta atcatggtag
      601 tttgacaagc cgtgaagtta cagtactgcg tgaaattgag atgctcgaaa atatggattt
      661 gctttctaac taccagttag aggagttgtt agataaaatt gaagtatgtg catttaatgt
      721 ggagcatgca caattgcaag tgccagagag cttacgaaca tgccctgtta cattatgtga
      781 accagaagat ggggtattca tgaggaattc aatgaattca aatgtttgta tgttgtatga
      841 taaaatggca ttaatacatc ttgttaaaac aagggcggct catcctttga gcagggaatc
      901 aatcgcagtt tcaatgattg taggaagaga taattgtgct tttgaccctg acagaggtaa
      961 cttcgtttta aaaaattaag atagcaatgg cgggttaact tttcgtaaaa tgaaattaat
     1021 tctcggtacg gcatgtaaaa cgcggcacct cgtatgcaag aacgtactgc ggttggttgg
     1081 tgaactttcg atagtgctag tattggatga tttccagccg ttaccgattt tatataactt
     1141 cagtggaatt attcaacaca caggcaaaac ggaataagct gagaaaaaaa gcgtgctttc
     1201 ccaatctggg gtaaatcacg tctgaatgac acctgaaaac aggtggtggc ctcaagaagg
     1261 cttggaatat tcttctttaa atgttatgta atttattgat ttttcgtgta cgattttaag
     1321 acatttattc caagaaaaat ttttaactct ttgatttttc gactctgtat catcggtctc
     1381 gaaacccgta gccacgtcgt atgcaagaac gtgccgcggt tggctggtaa gcttttcgat
     1441 agtgcgagta tctaatgatt tccagccgga actgattcta ttggaaagaa atcatgggcg
     1501 caatttacag atcacagatt gattagttat agatttttac tgtggcaaaa atgggacaaa
     1561 aggccgcaaa aggggcaaaa atggggcaaa aaaagtgtag attattgtgc tttattgtag
     1621 ttgttaatcc tgcctaacat attgaaaaac ataaaagtta ttgttcgtca gatagttgcg
     1681 gtttttactc tgacttgttc aggttgtatt gttctttctt actaatttct tgattttgcg
     1741 acatttaaaa gcgactcaat tcgttatatg gcatcagaag agtatgcgtc atgccggaac
     1801 gcccagcata agaaatctga tataaaaact gtggcgtgta tggtacggat tagaggggaa
     1861 aatgtcagca catttgcgaa atgaatcaaa aagcccgcag caatgtgcgg gcgttagtgt
     1921 cagcgcacaa ccagcacgga gcactctgcg tgacgcacta cagctgcggc gttagaaccg
     1981 agcagataag tggtgatatc cggtcgatgg gaagcaatga tgatcatatg agcggggatc
     2041 ttcttcgcca attccagaat gcggtctttt ggcgagcctt cctcaacatg gacatgcact
     2101 ctgtcggttg gcagtttaaa ttttttaatg atctcttcca gttgcgattt ggcttccgct
     2161 ttcaggtcat ccattgccgg taattctgcg gaatacgcta aacccagaga ggcatagtag
     2221 ggcagtgaag gtattaccgt caggaaatga acctctgcat catcaatctt tgcctctgcc
     2281 tcaacgtggc taatcacgcg ttgagttaat tctgaatcgg aaatatcgat agggacaaga
     2341 atcgttctgt tcataaaacc tcctgtttta gtatccgcat aaagtgtaac gccagatgac
     2401 actttttgtg taatgacgga gttcacattt ttaatttaga tcaaaggagg aagaataagc
     2461 agaaaaagcc cgccataaca gcgggcagga ggatttagaa ctgataaacc agacctaaag
     2521 cgacaatatc atcggtagag atgccgttgg cagcgtagaa actgtcatct tcatccaaca
     2581 ggttgatttt atagtcaaca taagtggaca tgtttttatt gaaatagtaa gtcgcgccaa
     2641 catcggcgta tttaaccaga tctttatcat caacacctgc cgggttgtct gcaccaccgg
     2701 cagcgtgcag gtcacggcct ttagacatca ggaaagagac tgccggacgc agaccaaaat
     2761 caaactggta ctgtgcagtg acttcaaagt tctgggtttt gtttgcaaca gcataatcgc
     2821 tgtcgccaaa cggggtcata ttacgcgttt ctgaatacat ggttgccagg taaatattgt
     2881 tagcatcgta ttttagccca gcagtccacg cgtctgcttt atcaccaccc gccgcagtat
     2941 ggttaacctg gtcattggtg cggtcagaag aggtgtatgc cgcaccagcg ctaaagccca
     3001 tgcctaaatc atatgttgtg gaaagacccc agccgtcacc gttttcatgg cgaacatcac
     3061 gtccgttgtt ggtgccttcc tgaccattac tggcaccttc gttgttacct tgatactgca
     3121 ccgcgaagtt cagaccattt accagaccga agaaatcagt attacgataa gtcgcgacgc
     3181 cattggctcg accagtcata aagttgtctg cattggtata agagtcaccg ccaaattcag
     3241 gcagcatatc ggtccagcct tcgatgtcgt acattacgcc ataattacgt ccgtaatcga
     3301 aagaaccgta atctgcaaat ttcagtccgg caaatgccag acgggtccat gactggtttt
     3361 ttgaagattc agtgttgttt gcctgaatat tgtattccca ttgaccgtag ccagtaagtt
     3421 gatcgttaat ttgggtttcg cctttaaaac ccagacgcgc atagctctgg tcgccatctt
     3481 tcgctgaatt atcagaaaaa taatgcaggc catcaacatt gccatacaga tctaatttgt
     3541 tgccgtcttt attataaact tcggctgcat gtgcagcacc tgcggcgagc agggcaggaa
     3601 ttaaaagtgc cagtactttg cttttcattg aataaatcct ttagttattt tatttgcctt
     3661 ttatccccat tttggggagt gaaaacatcc taaaggagaa gttcagttag ccgat
//
LOCUS       DAZFJF010000254         3691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFJF010000254 DAZFJF010000000
VERSION     DAZFJF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.256"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(78..1616)
                     /gene="cadC"
                     /locus_tag="ACHKAU_005043"
     CDS             complement(78..1616)
                     /gene="cadC"
                     /locus_tag="ACHKAU_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO5533920.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1876..1990)
                     /locus_tag="ACHKAU_005044"
                     /pseudo
     CDS             complement(1876..1990)
                     /locus_tag="ACHKAU_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2365..2514)
                     /locus_tag="ACHKAU_005045"
     CDS             complement(2365..2514)
                     /locus_tag="ACHKAU_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5533921.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
     gene            complement(2514..3004)
                     /locus_tag="ACHKAU_005046"
                     /pseudo
     CDS             complement(2514..3004)
                     /locus_tag="ACHKAU_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            complement(3096..3671)
                     /locus_tag="ACHKAU_005047"
                     /pseudo
     CDS             complement(3096..3671)
                     /locus_tag="ACHKAU_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
BASE COUNT         1014 a          784 c          789 g         1104 t
ORIGIN      
        1 gcaacatatt ataccaacag aaacatacaa aaactcaaca acaaatattc ccgagcataa
       61 atcaacccgg gagttactta ttctgaagcg agaaatttgt cgagataagg tacaacataa
      121 ggaacagaag tctggaatat accattttca atccagtaaa gggtgtttgc ccctgggcgt
      181 aaattaaagg cggtgagata tgcatcagcc gcttcccggt tcatcccctt catttcataa
      241 accttgccaa gcaacacata atttagccag gacatttcaa gatcaatgcc agtatttatc
      301 gcctggtaag actcatctgt tttacctttt accagagcac tgaccgcttt tatttgatat
      361 ataatggaca ggttattcaa ttccggcagt gtaacaatgt tgtctatttc tgtgttcagt
      421 gctgctaatt gtttttcatc taaaggatgt tgagaatggc gcacgatatc aactaatgct
      481 ttttctgctc tcgcgtaggt aaattctggg gatgattgaa caatctcacc taataattca
      541 ctggcacgat tcaatgattt atcatcgcca tgcagtaaat aatcatgtgc ctgataaaaa
      601 ttagttaata acgcaccacg atgaggcaat atttgctgga gcgtctcctg cattcgttgt
      661 ggccacggtt ggtttaacgc ttttgataaa ctctccagta aatcattttg aatcgccagc
      721 tgattaccgt tagtgatgac ataacgttta tccagcatgg ttgaaccatc tgcattgtct
      781 accaatttta tcgacataaa gcattgttga gcacggtatt ggcgctgatt aacaaacgca
      841 atagataatg ttttaccgga actgctcggt tcatcaatgt tgtagttgat tttgtcatgc
      901 accataaagg tggagaaggt gttaagtgat gtcgccacca aatcacccac gcctatcgcg
      961 taagagagct gatacgggga actccagctg ttacaacttt tatttaccat attaatgtca
     1021 atatcgcgtg gattgagcaa aatacgcgat ttgctcatag gaagacgtgt atcaagagtt
     1081 gaaaacgcta ccagtgctac acagatacct aacgacaaca ggaaaaaaaa ccatacccaa
     1141 aaggtagtga atcgtttgct tttaactggg gattgttcag gtggcgttgc ggtgttttga
     1201 atgttaagac tgtgggaggg agaatctgtg gcaggaaccg cctctggtat agggggaggc
     1261 gaagatagca ttatttcctc tccctcttct tcgctgtacc agataaccgg caccattaat
     1321 ttatagccgc gctttggtac agtagcgata tagacaggac tatcttcatc attatctttt
     1381 aatgacttac gtagttctga gatactctgc gtcacaacat gattggtgac aatacttctc
     1441 ttccagacat tatcgataag ttcatccctg ctaagtactt cgccactgtg ttgagcaaag
     1501 aaaaccagaa gatcgattaa tctcggctca agggtaagtt gacgcccatt gcggctaatt
     1561 tggtttatgg acggagtaac aagccattcg ccaacgcgaa ctacaggttg ttgcataata
     1621 gaaactcatt cgaaaaggga atgatgcaat gataattgcc acaacctatt tttaccatct
     1681 atagatgggt ttatttacat attattggtg aatgcaagac gttattttta ccagccataa
     1741 acttcttgat tacatagtat tacgaaagga ttttactgag aaccagaagt aatattcctt
     1801 accatcaaaa ttcatcatct ttgccaaaga aaaatgttca gaaaataatc catggaaaat
     1861 tgtccggagc gcttactatt ttaatggatt gttagtcttt gcatgagcaa gcggactgat
     1921 acatttctct ttgttctcat tcagaaaatc tcatcagtcg gcgttcagac cggtgttgtg
     1981 ctttatggat gcgttacgct cctgatgacg tcatttgacg ttcaacaaca tcacggggcc
     2041 gcacgacatt tcacgtcagt tagtgctatc gctcaggaac aaattttccc gaattgggat
     2101 atgcccgcaa attgctggtg atgtggggag aatctggttg agttcgggag aattgatttg
     2161 gaggcagaac gcttaaattg tggcgtccag aaacgaaaaa cggacctccg tggaggtccg
     2221 ttgatatgaa tttggtgccg gactcggaat cgaaaatttc ctttatcata ttgatattaa
     2281 tgcaatttgt ttggtttgat taaaaatgca ccctcaatta tacccgcagt cccttttgat
     2341 atcgaaaggg aatcacactc gggatcaggc tgagagcaca gaaggaaagt acctggcttc
     2401 cctggcctgt tcaatacagg gcagcagtgc tgccgaatcc gttttatcct catcgttgtc
     2461 aaagataatt cccgattcgc agtcgatatt gtcctgcagc cacgcaatga ggatatacgg
     2521 agaagcacat ccgtatcatc agtgtgacga ctgcggcggc catccatcca gtcatcggtt
     2581 cgtctgagaa tgacgtacaa ctgcgcacgc gacacccgga gacaacggct gactaagctt
     2641 actccccatc cccgggcaat aagggcgcgt gcgctatcca cttttttgcc cgtccatatt
     2701 caacggcttc tttgaggagt tcattttcca tcgttttctt gccgagcagg cgctggagtt
     2761 ctttaatctg cttcatggcg gcagcaagtt cagaggcagg aacaacctgt tctccggcgg
     2821 cgacagcagt aagacttcct tcctggtatt gcttacgcca gagaaataac tggctggctg
     2881 ctacaccatg ttgccgggca acgagggaga ccgtcatccc cggttcaaag ctctgctgaa
     2941 caattgcgat cttttcctgt gtggtacgcc gtctgcgttt ctcaggccct aagacatcaa
     3001 tcatctgttc tccaatgact agtctaaaaa ctagtattaa gactatcact tatttaagtg
     3061 atactggttg tctggagatt cagggggcca gtctagtttt ttgttatgtt ggctgagtga
     3121 caggatgtta atctgatgtc ttctacttca tgaagattat gttctaccag acgattgacg
     3181 atatcagttg gtgataaaaa ttcatctcca cacttaagaa gaggtaggcc agcagaacca
     3241 tcccctggca gataaatttt attgtatttt tctggtgtta aatcctttgg taggatagtt
     3301 ttcatctctg ttgccgcaat acttatagct gctgcggcag caacatcggt gttacttgat
     3361 tgtagataac ttctgtttgc accaggaaag gtaaactctg ttttcttttt atcaaaatct
     3421 tgttttgtag cttcgtgcag gaataatata tcataaggtt ttttccctgt tgtgactctt
     3481 gaaacatttt gaccaagaat attaagagca tattcaccta cggattttgt tcctaaaaac
     3541 acaagcaatc cttctttctc tggcactgtc tctatttttc tccctccagg aattgtggag
     3601 atggattgag attgttctgc cggaattgat acgctggcgc ttataggtag ctgagatctt
     3661 atactaaaca tacaacctcc ttcttagttt c
//
LOCUS       DAZFJF010000255         3524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFJF010000255 DAZFJF010000000
VERSION     DAZFJF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.257"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(116..191)
                     /locus_tag="ACHKAU_005048"
     tRNA            complement(116..191)
                     /locus_tag="ACHKAU_005048"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Ile,seq:cat)
     gene            complement(241..1290)
                     /locus_tag="ACHKAU_005049"
     CDS             complement(241..1290)
                     /locus_tag="ACHKAU_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5533922.1"
                     /translation="MINTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWEGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYVEQGRELKQHVMA
                     PLISYFRDARKSLGITSKQIAEATGKKNMASHWFGISQWQLPNEGDYNKLQALFARVA
                     AEKHQRGELEKPHHQLVSTYSELNRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIINASSRPGDLVADFFMGSGSTVKAAMALGRCAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(1441..1638)
                     /locus_tag="ACHKAU_005050"
     CDS             complement(1441..1638)
                     /locus_tag="ACHKAU_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5533923.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(1951..2493)
                     /locus_tag="ACHKAU_005051"
     CDS             complement(1951..2493)
                     /locus_tag="ACHKAU_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5533924.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGSKTGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCYATCRNRIDMWL
                     RIAEFILYPLMRDAFSFTDA"
     gene            complement(2502..2867)
                     /locus_tag="ACHKAU_005052"
     CDS             complement(2502..2867)
                     /locus_tag="ACHKAU_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5533925.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGVKIYKIESE"
     gene            complement(2868..>3524)
                     /locus_tag="ACHKAU_005053"
     CDS             complement(2868..>3524)
                     /locus_tag="ACHKAU_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5533926.1"
                     /translation="GQLAHQNLSAWMIDVIRHAMNGSQERELSLAELSWWAVRNQVAD
                     ALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQRTKNLAPLPHAHQQNPPQ
                     EKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGL
                     GGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA
                     "
BASE COUNT          826 a          991 c          769 g          938 t
ORIGIN      
        1 ctgagctaac aacgcagaat accgataatg gaccgccatc ggggacccgc ccccgcacca
       61 acaaccctgt tatggtgtcg tctgctcttc ctgataagct aatggcggtt tgtgatggtg
      121 gcccttgctg gatttgaacc agcgacctgg cgattatgag tcgctcgctc tcaccactga
      181 gctaaagggc cgggagcaga ataataatgg tgcgtaatta attctgcaat ctcatccgtt
      241 tcaaacgatt aaatcctgaa cttccctgac tgtctgttca aagcgtccgg tctccagctc
      301 aacaccaatc gcacaacgcc ccagtgccat cgccgctttt accgttgaac ctgaacccat
      361 aaaaaaatct gcaaccaggt ctcccggacg actgctcgcg ttgattatct gctgcagcat
      421 ttctgccggt ttttcgcacg gatgtttccc tggatagtac tgcaccggtt tatgcgtcca
      481 gacatcggtg tacggaacct gcgccgtcac accgaaatac cgccgcaaat ttttatattc
      541 actcagcagt tccgtatact gccggttcag ctcactgtat gtgctgacca gctggtggtg
      601 tggcttttcc agttccccgc gctgatgttt ttctgccgca acacgcgcaa acaacgcctg
      661 caatttgttg taatcaccct cgttcggtaa ctgccactga ctgataccaa accagtgcga
      721 agccatgttt ttctttccgg tggcttccgc tatctgtttt gacgttattc ccagtgattt
      781 acgcgcatca cgaaagtaag aaatcagcgg ggccatgacg tgctgtttta gctcgcgccc
      841 ctgctccaca tagccatcat ctttcgggcg atacggtccc tgataatgtt ctgcaaacag
      901 aatgcgctct gttgccggaa aatacgcccg cagactttcc ttattgcacc cgttccagcg
      961 tccggacggc ttcgcccaga taatgtggtt cagcacatta aagcgctcac gcatcatgat
     1021 ttcggtgtca gatgccaggc gatgaccaca gaacaggtaa agacttccgg caggcttcag
     1081 tacccgccag aactgcgcca gacactggtc cagccatttc aggtaatcat cgtcgccctc
     1141 ccactggtta tcccagccct cgggcttcac tttaaagtat ggcgggtctg tgactatcag
     1201 atcgacagag ttttccggta aggtctggat aaattccagg caatcagcgt tgattaactc
     1261 acaactggat atttttacag tattaatcat agatcaataa gcacttctct gataggctca
     1321 tgccgctttt gcgcaaagca gatgggcctg aggtttgctt gtgaccccaa cgcatgagca
     1381 gatggctggc aggtgccgct aacacccacc agccgcccat taccacaaat taaaaagcct
     1441 tcactgcgga aggcgtctgt aacaaccgaa ctgataatct gccagacccg ccataacaag
     1501 ctgagtcagt attaactggc agcgttcgcg tgaaaggtaa gtattctgcg caatttcccc
     1561 gacggtcgcc ggttcggtga cgcttaattc attaaacacc actctggcgg tttcggtcat
     1621 atcctgctgt tttagcatgc ctttttccct tttctggtta acgtgacata ccaatacctc
     1681 ttgtcgaaaa agccagcaag ctgaaagacc agtattcaca actaccagcg cgtttaatgt
     1741 tctgtgccgt ttttcaggca taaaaaaacc cgcataaagc gggttctttc aggtgtccat
     1801 gtctgctatt cgcctcgcgg tacagctttg cgaagcgtag ctggattgaa acagtttatg
     1861 gctaaaaata caagcttttt ttctaaaact gcacaaacct tactaccagc caaaaatcct
     1921 cttcgtgcaa caacaaacgc cctccagatt ctaagcgtca gtaaaagaaa atgcatctcg
     1981 catcagtgga tacagaataa actcagctat tctcagccac atatctatac gattgcggca
     2041 tgttgcatag caccactcag ggtgaacctc attcaacaat tcagccattt tgcgtttact
     2101 catcccccgc ccttcgtatc tttgccgcag gatatcaatc aatccaggat aacgtgcaag
     2161 cgctttactt atcaccccat caatgcgtaa cgcctctgca tcagtacagt gagacaacca
     2221 gctcttctgt ctgccagcga tcatctctcg caagaatgct tccagctctg gtttatcaat
     2281 ccctgactcc ctgattctac gcagggcttc attgattgcg gtttttgtca gttttttgga
     2341 tgccagcaac tgattgaaca tatttcctgt tttgctacca cctatatacg accaacgccc
     2401 ccacatccgt aatttcccct ggatccagac ggcttccagc gtttttagac gtaaatgctc
     2461 gccgcttttg cctgtaattt ccgggtatat catatttacg atcactcact ctcaattttg
     2521 taaatcttca cgcccagccg cccaccagga acgagctgac cgcgcacaat attgatttca
     2581 tcaaactgct cgtcgtctat gagaagtccg gcatgcgtca acgcatccag tggtgctttc
     2641 aggatattgt ccaggtcacg gcggcgctta tccggtggtt ctgcaataat ttttattgcc
     2701 aaccttccgg acaggcttaa tttcagccgc tgctggcgaa caataagcgc cactgcccgg
     2761 cgataacgct ccccggcttt tgatacaaaa tatgtgctgc cacgacgacg ccagtaagtg
     2821 ttcaccgttg gcgggtaagg caaaacaaat tctatgcgtt cagtcattta tgctttccac
     2881 ttcagaacac ccgaatttct cgcgtgcatt aaaaaacgaa tcagcaacaa cagctggctg
     2941 ccgtgttttt cttcaaaatc ttttaccccg gcgtgtagtt cgctatggca tttacggcac
     3001 agcggaataa caaacaaatc atcagccttt gttcccatcc ctcccagtcc atgaccaatg
     3061 atgtgatgcg gatcatctgc ctgattgcca cacgtcatgc atttctgcgt ttttacccag
     3121 cgcgtgtata caggcatctc ttcccgttgt gatttctggc gctggagata ctgagccggt
     3181 gactcgggat caacggcaat gctgaccacc gtcttttcct gtggcgggtt ttgctggtgg
     3241 gcgtgaggca gcggcgcaag attttttgtg cgctgcttca gtatgatggt ggcggtctgc
     3301 tctcccggta cgatgtcgct ttcacggtac attgagcgga ttttttccgc acgcaacccc
     3361 agcgaacgac gtaataccgc ttccggtagc gcgtccgcca cctgattgcg gaccgcccac
     3421 caggataatt cagccagcga taattcccgt tcctgcgagc cattcattgc atggcgtatg
     3481 acgtcaatca tccatgcaga caggttttgg tgagcaagtt gccc
//
LOCUS       DAZFJF010000256         3461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFJF010000256 DAZFJF010000000
VERSION     DAZFJF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.258"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(638..835)
                     /locus_tag="ACHKAU_005054"
     CDS             complement(638..835)
                     /locus_tag="ACHKAU_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5533927.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(901..1171)
                     /locus_tag="ACHKAU_005055"
                     /pseudo
     CDS             complement(901..1171)
                     /locus_tag="ACHKAU_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1476..1664
                     /locus_tag="ACHKAU_005056"
     CDS             1476..1664
                     /locus_tag="ACHKAU_005056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533928.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1992..2186
                     /locus_tag="ACHKAU_005057"
     CDS             1992..2186
                     /locus_tag="ACHKAU_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533929.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2529..2657
                     /locus_tag="ACHKAU_005058"
     CDS             2529..2657
                     /locus_tag="ACHKAU_005058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533930.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2967..3302)
                     /locus_tag="ACHKAU_005059"
     CDS             complement(2967..3302)
                     /locus_tag="ACHKAU_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533931.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1037 a          781 c          747 g          896 t
ORIGIN      
        1 aaccaagtat attatcttct ggtagagatt caggatacag tttgtaggag ctttcttttt
       61 tgaacaatga tttgcaggac agttatgcta aactagcgga atcagtatca caacaataaa
      121 tgactgatca tacctgaaat ttgaattaac aggcatcatg aatgtcaata gtctaagcgg
      181 tgactactta tatcgcttgc ataaatacta aacactgact agaaaagtat tggataaccc
      241 cgtcactaaa agtaacgctt cggttatttg ctcggaatgt attaacgcca agattgttag
      301 ccgttacgcc ttatatcact ggcgctgaca aatgtttctt ttctaattta ctgaaccggc
      361 gcagacacgc aggaaagcac gccggcaccg gtgtaatgcc agttctaatc aataagaatg
      421 gaatgacgaa tcagtcagga agggataaca acccgctgct gaacggaagg tatcagcagg
      481 aagatgaacg ggaatatcgc cagcaccaag ggcagggccg ggttccacaa taccagcgct
      541 ggcattttgc acattgccag cgtaccggca ttacaacacg tttcatgctg ctgcccccca
      601 ggattcctcg attcgttgct acatccactg ctctacttca cttccactgc tccacttcac
      661 ttttgtacca gcggctgctg cgccccagtt tgatgggttt agggaaatgg ccttcactga
      721 tcaacttgta aaaccatttg tcggtcatac cggtgaaact ggtgatatag ttcatgtcga
      781 tcagggaatt atcggcacgc agaaatacgc tgtcgtggct tgttgtagtc aacatggtca
      841 gaatgtcctg tgagatttat gaagattgtg cgaatgcggg aaatctactg agctgtgctt
      901 tcagaactgg cctgttacgg gataccaggg attaccggcg gggtaacggg cttccggatc
      961 atacacacca cgattatcgc ggacaaaatc actgaacgcc catatcacct ctttaagtat
     1021 gtcttcgcag tccggtaaat gacgatccag cgccacatcc cgggtggtac agctttgatg
     1081 cgcccggtga cacaaagccc ggattgttcc agacatcctg aatcaaacgc cccagattag
     1141 gggcgtcgaa atatgcctct ctgaccatta tattccggcg tataggtagc aggtctgaag
     1201 tgacaatgcg tcacctgacg ttaaaagtca cccaagatga ccccagtggg ggcaacaagt
     1261 ataaaaccgt tttttgatta agtggtcgga tatgaaaagg ggaaggttta tgtgaaaaat
     1321 acctgtgttt tctttgaaaa cacacaacag tatcaggtta tttatgttta tacagtgcgt
     1381 tccaaactga cctgaataat tacgcaagtt gcaacaataa aacatttttg ccgtagtgtg
     1441 aggtactccg gtaaattgct tcccaggtgc agacaatgac taacgaagaa ctacaggcaa
     1501 tcgcattatc catgcttgag cgccagcgcg aaaaagtaca ccagcaacga gagtctttgc
     1561 ttaacccatc tccattcacc aggccaaaac gattggagct tgaagcattc ttggacaaat
     1621 acccacggcg gcttaaatca ggcaaaacca gaccaccagg ctgatagctc acagtttgtt
     1681 ctccgattca ataaaacgga taagaaccat cagcgcggta gcgtaaggca ctacagcgca
     1741 ttgctttacg agtaataaca atggatttat caccagaaga cgcaaagttt ttacgagaac
     1801 tgcaacaccg tatgctcaga gaagccatta tcgaacagca aacgggcatg agcgccatag
     1861 acccattgcg atacaaacgg gcctcatctc ctttagttga cgacaattcc aatgcggata
     1921 aaacacgtcc accacactga ttacattttt cattagcaca gtggtgtgat gcagcatacg
     1981 aggaaaaccg gatgaatgag aacaaaataa aaaggctgga gcaactgtta caggcacgcc
     2041 aacgggagtt tgccactaaa tggggacgtg ccgcgctgcc acatgaacgc ctaacctatt
     2101 ttccacttga agacctcatt aaaaataatg gcaaaaaaat caggtctgat aagaaacact
     2161 tcttagaatg caatgaagac aaataacaac aatctgcata ttgccaccaa ccacagatgt
     2221 ggcatcgtta tcagcgcact tgattttctt tatatccact gacgagtttg ccggtcattc
     2281 tggtatcatg cagatattta cggcgtacaa gcacactagc atgacattgt aataaggcct
     2341 gagcctaacg ctttaatgtg tgtcatacac tcagtggcgt aatcgttgac aatatggtgc
     2401 atgtaatcgt aagcgtccag cgaaatccat ctggtcataa tctgaagcat ccgacaaagt
     2461 ggtgtccacc aaataagtga tctgcgccca atatcctgag ccggaacttt gacaaaggcc
     2521 aacgaaaaat gggagcggat gttacagagt tctcggtaca ggggaaaagt ggtacctgtc
     2581 gccagttctc cgatctgttt aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg
     2641 tgatggagca tcgttaatac gatgctatac gatgcattca cagtgcccgg ccagaggatg
     2701 ccccgtcgct gcatatggat cagggttggc aatatcgaat tgcaggctat aggcaaagtt
     2761 aaagccccat ggagtagcac aaaatatgcc gcacaaagga aacggtctga ataacgcagt
     2821 gatgaagaac ttcttcagca cactgctaaa acgcatagtg atcgagcgct gattctggcg
     2881 aacaactgaa ctaatacatc agaatctgca ttatgttaaa taaatataaa aagatggttt
     2941 aaataccccg ttacttttga cttacactat acggtatcgc atcgtttaat attcgcaccg
     3001 gccagatttt tatttctatt agttgtcaca atactgaatg cgtacgacca cagtattctg
     3061 gctcctgtgt ggttatgctt taattctgcg ttccgggcag ataagcagtt gcttgcagga
     3121 atccttcttg tgttaatgtc agttcccctt ttaccagtgc tgatttccac attccgtcca
     3181 acagagctta tagcctttcc ctggattata gcattgtccg gctgaagttc tttttgaata
     3241 ataatagaag cactgctggc agatccagtc cgtttttcat aacccactgt actgataacc
     3301 ataatctaat cagtagaaat tgagtcgaaa ataagcacta ctccatacag gataattaga
     3361 ggtcagtttg attattcaca attcatcatc agcattttct atttctgacg aaatcaatat
     3421 gaaaataacc atatatgata attattataa taacggcttt a
//
LOCUS       DAZFJF010000257         3395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFJF010000257 DAZFJF010000000
VERSION     DAZFJF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.259"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="ACHKAU_005060"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="ACHKAU_005060"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(209..736)
                     /gene="mobB"
                     /locus_tag="ACHKAU_005061"
     CDS             complement(209..736)
                     /gene="mobB"
                     /locus_tag="ACHKAU_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO5533932.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(718..1302)
                     /gene="mobA"
                     /locus_tag="ACHKAU_005062"
     CDS             complement(718..1302)
                     /gene="mobA"
                     /locus_tag="ACHKAU_005062"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO5533933.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1372..1641
                     /locus_tag="ACHKAU_005063"
     CDS             1372..1641
                     /locus_tag="ACHKAU_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO5533934.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1718..2704
                     /gene="srkA"
                     /locus_tag="ACHKAU_005064"
     CDS             1718..2704
                     /gene="srkA"
                     /locus_tag="ACHKAU_005064"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO5533935.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2721..3347
                     /gene="dsbA"
                     /locus_tag="ACHKAU_005065"
     CDS             2721..3347
                     /gene="dsbA"
                     /locus_tag="ACHKAU_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO5533936.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          858 a          818 c          833 g          886 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa attaagcaga
      121 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
      181 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      241 caaaatcagc cagcccctca acatcattga tatccagtaa tgcgacatca agattaagcg
      301 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      361 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      421 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      481 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      541 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      601 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      661 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      721 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      781 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      841 ttctcctgct tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      901 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      961 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
     1021 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
     1081 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatggcgatt
     1141 ggcattaacc acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa
     1201 tggtttgccg tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc
     1261 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1321 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1381 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1441 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1501 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1561 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1621 cgcggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1681 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1741 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1801 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1861 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1921 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1981 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     2041 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     2101 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2161 tctgaatgaa tatctcattg agccacgcaa gctgtttgag gacgctacac tgataccttc
     2221 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcgca
     2281 ctggcgggaa gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2341 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2401 ggatttgtgg atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat
     2461 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2521 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2581 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctgc gacaaacggc
     2641 gacttttata gaacaggcaa aagttctaca agaaccccct ttacaattaa cacctatgta
     2701 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2761 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2821 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgtccg cactgctatc
     2881 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2941 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     3001 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt cactgttccg ctgtttgaag
     3061 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3121 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtc aaatctctgg
     3181 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggcgttccg gcgatgtttg
     3241 ttaacggtaa atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg
     3301 ttcagcagta tgctgataca gtgaaatatc tgtccgagaa aaaataattc aatgtaaatt
     3361 acataaggcc cgtgaatatt cacgggcttt tttta
//
LOCUS       DAZFJF010000258         3350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFJF010000258 DAZFJF010000000
VERSION     DAZFJF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.260"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(53..853)
                     /locus_tag="ACHKAU_005066"
     CDS             complement(53..853)
                     /locus_tag="ACHKAU_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533937.1"
                     /translation="MEMVVSDGEERFTELDFYYGSKALEGTSEIVTVLTNTILNREVV
                     TKIPSIEGITASFRTSYEGSFRQRFMLKITGEEQRRNYGAIGEGAFLDVMSYYLTRPL
                     LTPYNFTYKRAARIVKELESDYSSLMKRLYGPLLKLHKPIEEQNYTVELKRRRTNLAR
                     YDHTTLENLIVERADDERIIIEASITRFNRLTGTGRLILEEDSESVSFEPSYLWRDFP
                     ADQKRWLSRNLDVNTDNGDFTRVRLEVTSIRNFLGVIKKYSLHRVITD"
     gene            1652..2125
                     /locus_tag="ACHKAU_005067"
     CDS             1652..2125
                     /locus_tag="ACHKAU_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533938.1"
                     /translation="MDIDTLGGQELRDKIFAGLKIYEGKPFIERFGLFMGKAQLLEFG
                     LKKILVSFPGYNLDEEKLERLTLGQTRVELKKLGLRTDYNAYLKSFKDQRNTMAHEFL
                     ANFAVTQQLLDGAALIRTFERELDHACYAVEQLIILFDFINGAGDVTAWLEPTAP"
     gene            complement(2426..3280)
                     /locus_tag="ACHKAU_005068"
     CDS             complement(2426..3280)
                     /locus_tag="ACHKAU_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HFO5533939.1"
                     /translation="MNTNIGMRYIDVVANGNAGEFYFAYWISSNFIWPCRILDIDMGL
                     DAQVEVYDDQNHSTGMFIGVQVKTTATTLEENPNVSVPMKNIAYWESINDPVVIIRTC
                     LNNNSKEPSLYWKHLPKSELRKYLNRAEEKGYETVTIMFEEDKNSLLKTDKSAWLKIF
                     LSDYDKEIIESVVGIKSKLDKLGKYLKDNYIDEQFICGFPSDDFPSELNDTLNKYDEL
                     AVAVKVNPRLKYLSNEVKSTIDSYNSYINLILLFFKEGWSTGSIRNSDFDISSTINPA
                     LHKIINSY"
BASE COUNT          970 a          713 c          608 g         1059 t
ORIGIN      
        1 ataaatcaac catgtaactg atataactaa aaaggcaaca tactttatca tatcaatccg
       61 ttataactcg gtgaagacta tattttttaa tgacaccaag aaagtttctg attgatgtca
      121 cctctagccg tacacgagta aaatcaccat tatctgtgtt gacgtctaag tttctagata
      181 gccagcgttt ttgatctgct ggaaaatctc tccaaagata tgaaggttca aaacttacag
      241 actcagaatc ttcctcaaga atgagccttc ccgtccccgt tagccgatta aatcttgtaa
      301 tagaagcttc aataatgatc ctttcatcat cggcacgttc aacaattaga ttttcaaggg
      361 tcgtgtgatc ataccgagca agatttgtcc ttctccgttt cagctcaaca gtataatttt
      421 gttcttctat gggcttatga agcttcaata gtgggccata aagcctcttc attaatgaac
      481 tataatctga ctcaagttct ttaacaattc tagcagccct tttatatgtg aaattataag
      541 gtgttaataa tggtcttgta agataataag acataacatc tagaaatgcc ccctctccga
      601 ttgcgccata gtttctacgt tgttcctcac ctgtgatctt gagcatgaaa cgttgtctaa
      661 acgacccttc ataactggta cgaaaagatg cggttatgcc ttcgattgat gggattttag
      721 taactacttc gcgatttaga atcgtattag tgaggactgt aactatctct gacgtgcctt
      781 caagtgcttt tgacccatag taaaaatcaa gctctgtgaa tctttcttct ccatcactta
      841 ctaccatttc catgggaaca tgaatttcca ttctctaatt ccaaataaaa accatgggtt
      901 taatatgtcc taattgattt ggttttggca actatttttg attgtttttc aagaataatt
      961 cgaaagctat taattaatgg ctggaactaa taggttccag ttttcaacta atccagtgac
     1021 ctgctccccg ctgatcaact cagactgttg ttagcaactt acgctcctgg tacggagcgg
     1081 gcattgatta cccgctccgt tcacgttccg cctggaagtt ctgcaattta tgtctatcgg
     1141 ctattgaaaa ccttccggtc cttcccaata tcgaaactgg cacctttgcc agataacaaa
     1201 gagatacggg aacatgatgt acgtctacag aagacctcca aatcagctcc tttccatttc
     1261 tcgtttgaag cagtatttgt tgcatgaggt ggaatcaaat caacacatca tgagacagtg
     1321 ggaatctgcc agaacggcac ttcgaaacat ccgtaacaaa gggcatgatc cagacaccgc
     1381 ctcctgggtt tcacttaaaa atgatccccg agtcttcacg aatggcgcag atgaaaagca
     1441 tgggttcatt tttgttgacg acgacgatca atcagtcctt gttgtggcct cctgggtgat
     1501 agatgagtac ggttcccccc tcactacata ttaatccctg tacctggaca gaagagtttt
     1561 ctgtccacaa ctaccaatag ctttacataa tgtgtgcatc taacttcaat aagttacttc
     1621 attaccaata atgtctaaaa aggaaacgcg catggatatt gatacgctgg gtggccagga
     1681 acttcgggat aagatttttg ctgggctcaa aatatacgag ggtaaaccct tcatcgaacg
     1741 gttcggtctg tttatgggca aggcacagtt gctagagttt ggcctaaaaa aaattcttgt
     1801 ttcttttccg ggatacaacc ttgatgaaga aaaattagag aggctgacat taggccagac
     1861 ccgcgttgag cttaaaaagc tggggctgcg cacggactat aatgcttacc tgaagtcatt
     1921 caaagatcag cgcaatacga tggctcacga gttccttgct aattttgcgg tcacacagca
     1981 gcttttagac ggagctgcac ttattcgcac atttgagcgt gagttagacc atgcctgtta
     2041 cgcggtggag cagttgatca tccttttcga cttcatcaac ggtgccggtg acgtaaccgc
     2101 ctggcttgag cctactgccc cctgatactt cgtatgcttt tatctctagg ctggacagaa
     2161 tagtaattct gtccaggtat gcagaatggc tgtctatata tcttcattag ttcacagtag
     2221 gttaccggcg gctttgagcg aacagcggaa cttcgccctt taataaatga taataaattt
     2281 tcattatcac tttagttgaa aatggtgtcg aagatgaaga gaaatgtcat attagcctac
     2341 ggagtacttg tgaaataaat tcatagcatt ccctaacatc taaacttagg gctcactgaa
     2401 cgatggtgaa ccctatgcca ttgggttaat aactattgat aattttgtga agtgcagggt
     2461 taattgtcga tgaaatatca aaatcggaat ttctaatcga cccagtgctc catccctctt
     2521 tgaaaaagag cagtataagg ttaatatatg aattgtaaga atcaatagtt gattttactt
     2581 cattcgaaag atattttagt cgagggttta ctttaacagc aactgctaat tcatcatatt
     2641 tattcaaagt atcgttcagt tctgatggga aatcgtcact tggaaaccca caaataaact
     2701 gctcatcaat ataattatct tttaagtatt tacctaactt atctagttta cttttaatcc
     2761 ctactacact ttcaatgatt tccttatcat aatcgctcaa aaatatcttt agccatgctg
     2821 acttatcggt tttcagcaat gagttcttat cctcctcgaa cattatggtt actgtttcat
     2881 aacctttttc ctcggctcta tttaaatatt ttcttaattc acttttcgga agatgcttcc
     2941 agtacaagct aggctctttt gaattgttat taagacaggt ccgaataata acaactggat
     3001 catttatgct ttcccaataa gctatatttt tcatgggtac agaaacatta gggttttctt
     3061 ctagcgtagt cgcggttgtt ttcacctgca caccaataaa cattccagtt gaatgatttt
     3121 ggtcatcata aacttcaact tgagcatcta accccatatc aatatcaaga atacggcaag
     3181 gccagatgaa attacttgat atccagtatg caaaataaaa ctctccggca ttaccatttg
     3241 ccactacgtc tatatatctc ataccgatat ttgtattcat taatactccc ccacggctaa
     3301 atactatttc ccctttgtat attacaatgg tttaacgcaa tcaactgcta
//
LOCUS       DAZFJF010000259         3337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFJF010000259 DAZFJF010000000
VERSION     DAZFJF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.261"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..3072
                     /gene="agn43"
                     /locus_tag="ACHKAU_005069"
                     /gene_synonym="flu"
     CDS             <1..3072
                     /gene="agn43"
                     /locus_tag="ACHKAU_005069"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO5533940.1"
                     /translation="TGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPG
                     ETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSG
                     GLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQV
                     VKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRTEGTANTTV
                     VYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQK
                     GRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLD
                     VLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIG
                     GGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKT
                     VNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDS
                     TVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAG
                     QIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTIL
                     NLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESW
                     YLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHD
                     NNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSR
                     AGTVRDDAGSLGGYLNLIHNASGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLE
                     TGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDM
                     TFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSP
                     SQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            3193..>3337
                     /locus_tag="ACHKAU_005070"
     CDS             3193..>3337
                     /locus_tag="ACHKAU_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533941.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAG"
BASE COUNT          769 a          912 c         1023 g          633 t
ORIGIN      
        1 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac ggggtaaacg tggcggtgtg
       61 gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg tgctggctgc tgacatcgtt
      121 gtgcacccgg gagaaaccgt gaacggcgga acactggcaa atcatgacaa ccagattgtc
      181 ttcggtacga ccaacggaat gaccatcagt accgggctgg agtatgggcc ggataacgag
      241 gccaataccg gcgggcaatg ggtacaggat ggcggaacag ccaacaaaac gactgtcacc
      301 agtggtggtc ttcagagagt gaaccccggt ggaagtgtct cagacacggt tatcagtgcc
      361 ggaggcggac agagccttca gggacgggca gtgaacacca cgctgaatgg tggcgaacag
      421 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc
      481 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg
      541 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc
      601 aataaaaacg gtcgccagat tgtgagaact gaaggaacgg caaataccac tgtggtttat
      661 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac
      721 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag
      781 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcag
      841 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta tggattcgtc
     2281 cgtctggagg gtgacctgat gagaacagag gttgccggta tgtctgtgac cgcgggggta
     2341 tatggtgctg ctggccattc ttccgttgat gttaaggatg atgacggctc ccgtgccggc
     2401 acggtccggg atgatgccgg cagcctgggc ggatacctga atctgataca caacgcctcc
     2461 ggactgtggg ctgacattgt ggccctggga acccgccaca gcatgaaagc gtcaacggac
     2521 aataacgact tccgcgcccg gggttggggc tggctgggtt cactggaaac cggtctgccc
     2581 ttcagtatca ctgacaacct gatgctggag ccacaactgc agtatacctg gcagggactt
     2641 tccctggatg acggtaagga caacgccggt tatgtgaagt tcgggcatgg cagtgcacaa
     2701 catgtgcgtg ccggtttccg tctgggcagc cacaacgata tgacctttgg cgaaggcacc
     2761 tcatcccgtg cccccctgcg tgacagtgca aaacacagtg tgagtgaatt accggtgaac
     2821 tggtgggtac agccttctgt tatccgcacc ttcagctccc ggggagatat gcgtgtgggg
     2881 acttccactg caggcagcgg gatgacgttc tctccctcac agaatggcac atcactggac
     2941 ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt tcaggccggt
     3001 tatgcccaca gcgtcagcgg cagcagcgct gaagggtata acggtcaggc cacactgaat
     3061 gtgaccttct gacagaacca tcgcctctct gtggtcccgg tcatcatgac cgggacccgg
     3121 accggcgcaa cggatcttca acgccacatt cgctggcatt aacaataaca tgatattcat
     3181 cacggagtga ctatgttaca gatagtcggc gcgctgatcc tgctgatcgc aggatttgcc
     3241 attcttcgcc ttttgttcag agcattaatc agcacggctt ctgcgctggc agggctcata
     3301 ttgctgtgtc tgttcggccc ggccttactg gctggct
//
LOCUS       DAZFJF010000260         3178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFJF010000260 DAZFJF010000000
VERSION     DAZFJF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.262"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..595)
                     /locus_tag="ACHKAU_005071"
                     /pseudo
     CDS             complement(32..595)
                     /locus_tag="ACHKAU_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(829..1506)
                     /locus_tag="ACHKAU_005072"
     CDS             complement(829..1506)
                     /locus_tag="ACHKAU_005072"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO5533942.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            1704..>3178
                     /locus_tag="ACHKAU_005073"
     CDS             1704..>3178
                     /locus_tag="ACHKAU_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFO5533943.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAA"
BASE COUNT          774 a          804 c          751 g          849 t
ORIGIN      
        1 tattcaacat aatttgatgc cgatttgttt attattggca tatgtttgta tcctttctat
       61 aaattttccg cctgacagtg gaaataatat attctactga tttttgtagt ttaaacgatt
      121 tatgaagaat tttactgttg tacagtacca gatactcagc cagtttcaga ttgaacatat
      181 tcagctattc aaagagcaat tcttcattaa actcaataaa ttgttccctc agtgttcggt
      241 taaaatgctc acttatggca tttattttcg gcgtatagag ataagtccat acgaacccga
      301 cggtggcttc ctgtagcgtt ttgtcgaatt ttcccgagaa ctctttgccc atcatcagtg
      361 atgatctggt tgataccaac aaggaacaac tgggctacac agcagaagaa atgcctgatg
      421 atatcacttt taagcgatag tacggccaga gagtagttgc tgtgttcgtc gagcatggta
      481 atgaaataac ggtacagctc gccattctga gctcaatagt atccatgcca ctgagcttac
      541 aggtctttat cgggcgatag tgctttattc ttcggggctt tgtggatgat ttttcgaaat
      601 gttcatgata gttaggtaaa gctaccgcat atcatttgca aaatgacaga tagatatact
      661 tcaatttgaa tcttaacaat tagtatggga atgctgtcaa tcctctgtat gcgtccacca
      721 catattgaaa tttttgttct ctaatatatt ccacgggcgt caagagtgtg aggcctgtgc
      781 tgttatacag caccagacgt tgtcccccat ctttgacctg tctcttgatc agatctcctg
      841 atcaagagac ttcatcacca ggtaaccctc agccatatcc tgatgtctga accagccatc
      901 ccacataact acccaaccgg ggctgcttgt gcgtttgctg tcatgccatc gccccagttt
      961 agccagtttc aaacaggccc atttcagggt cggtgtttgc gctggaagct gctttccttc
     1021 cagcttaacc cacagtagtt tccactctgt cggcgtcagt attttctcac agctgtcatt
     1081 ttgtgtttct tcactgatac ttccctgccg caggcccagc acccgcaccg cgataaccac
     1141 catgcgctca aggttatccc gggtctgcat tcgcagcgat tccacacatg taccaccact
     1201 tttccacgct ttgtggtatt cctctatcag ccagcgttgc tcgtaatggc tgacgatacg
     1261 tcgcgcatcg gcggcactcg ccactttttc tgatgtcagc ggataccagc aggctccgtc
     1321 ctctcctgct ccccgcaaca gacatacgtg agcgagagcg cctggccgct gttgtcggga
     1381 ttttttatgc tgacttcgct gtaactgatg agcatccggg cctggcgggc tgcccgcccg
     1441 cctttttgca tcacattcag cgtgtggctt cccgcggctg actttataat gcaggtaatg
     1501 ccagatatcc gcttcccggt cacagacagt gattacctgt ttctgtatct cccccagccg
     1561 ttcggccatg cgctccgaag cctgctgcca gcggtaactt tctttttctg tcacgaacgg
     1621 tgcaatagtg gttcttccgc aacttgggtg gaagaaaatg gatgaaactt tctggtgaac
     1681 aaagatctaa gctgttttaa gtcatgggca actcaatgca ctctcttaag acacttctac
     1741 agttaccttg cggatggcga tgcagtcgac aaattattag ctctgacggt ataaccctcc
     1801 atctccacgg aaaacgcaaa aaagcacaat gtcctgaatg ctttaagcgt agcgactctg
     1861 ttcatagttg tcgtcggcgc cggatacagc atctaccctg ctcaggacat acgctatggc
     1921 ttgtattttc cgtccgccac tggtactgcc gtaacccggc ttgttcacgt aaaatttttg
     1981 ccgggtcgct tgctcccttc gccggttcac accaacagtc ttcacaggcg ttacaaaatt
     2041 tacaacgtca actgggatta atcgccggag gtgaggccgg gaaacgggca gcaacggcag
     2101 tggggctccg ttgcagtgct gatactctcc ttcgcagggt tatcaatacc ccggagacga
     2161 aacagtcagg cgcgcctcat gtcggtattg atgagtgggc gtggcatcgg ggccaccgtt
     2221 acggtacgtt aatcgtcaat cttgatactc accgtcccct cgtcctgctt cccggccgtg
     2281 atcagcgtac gctggcgacc tggttcagaa aatatccgga aatacaaatt gtctcgcgtg
     2341 atcgcagtgg agtctatgcg acagcagcac gtgaaggtgc acctcaggcc agacaggtgg
     2401 ccgatcgatg gcatctgcta aaaaatattg gcgatgcgct tgaacgaatg atgtacagac
     2461 atatacctct gatacgtctt gttgccagtg agttgtcacc aaagaaatca cctgatccag
     2521 aaccatctgt gcctgcagca tcgctccgtc gtccggaacg cctaaaacaa caaacccgca
     2581 aaaaacggca tcagcgttgg acagaggtta tggccctgca taacaaggaa tgtagtttca
     2641 gggaaatatc ccgtattaca ggcctgtcgc gtgtgacagt cagtcgctgg gtgcgttcag
     2701 gaacattccc tgaaatgtca acccgacctc caaagcgagg gcttctggac ccatggaggg
     2761 agtggttaaa agagcaacga gaaagtagta attataacgc cagccggata tggcgggaaa
     2821 tggtggcccg gggatttaca ggcagtgaaa ccatcgtcag ggatgcggtt gccaaatggc
     2881 gtaaaggctg gaacccaccg gttactactg ccgtcagact tccttcagtg tcccgggtaa
     2941 gccgctggtt gatgccctgg agaataacca gggatgaaga aaattatgct tcccgattta
     3001 ttagtctgat gtgtgaaaaa gaacctgagc tgaaaatagc gcagcaactg gcactcgagt
     3061 tctaccgtat tctgaaaacc caaaataaat cacagctaag cagttggttc aatcgagtcc
     3121 acgaaagcgg ttcagcagaa tttcggcgcg tggcggcggg gatggaagct gatgctgc
//
LOCUS       DAZFJF010000261         3172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFJF010000261 DAZFJF010000000
VERSION     DAZFJF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.263"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..160
                     /locus_tag="ACHKAU_005074"
     CDS             <1..160
                     /locus_tag="ACHKAU_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533944.1"
                     /translation="KAYQKAWKEHRDRYQQDMEKLESENMELRRKLGEAKRDIDAYKR
                     LFNGESHA"
     gene            120..329
                     /locus_tag="ACHKAU_005075"
     CDS             120..329
                     /locus_tag="ACHKAU_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO5533945.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEYDG"
     gene            322..927
                     /locus_tag="ACHKAU_005076"
     CDS             322..927
                     /locus_tag="ACHKAU_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5533946.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHSRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            924..1130
                     /locus_tag="ACHKAU_005077"
     CDS             924..1130
                     /locus_tag="ACHKAU_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO5533947.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            1108..1773
                     /locus_tag="ACHKAU_005078"
     CDS             1108..1773
                     /locus_tag="ACHKAU_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO5533948.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            1770..2393
                     /locus_tag="ACHKAU_005079"
     CDS             1770..2393
                     /locus_tag="ACHKAU_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5533949.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            2646..>3172
                     /locus_tag="ACHKAU_005080"
     CDS             2646..>3172
                     /locus_tag="ACHKAU_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533950.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAY"
BASE COUNT          946 a          650 c          794 g          782 t
ORIGIN      
        1 gaaggcgtat caaaaagcat ggaaagaaca ccgcgatcga taccaacaag acatggaaaa
       61 gcttgaatca gaaaacatgg aattaagacg aaagctcggt gaagcaaaac gagacattga
      121 tgcttacaag cgacttttta atggtgaaag ccatgcttag cccatcccaa tcccttcaat
      181 accagaaaga aagcgtcgag cgagctttaa cgtgcgctaa ctgcggtcag aagctgcatg
      241 tgctggaagt tcacgtgtgt gagcactgct gtgcagaact gatgagcgat ccgaatagct
      301 caatgtacga ggaagaatac gatggctaaa ccagcgagaa ggaaatgcaa aatatgcaag
      361 gaatggtttc acccggcatt ctcaaatcag tggtggtgct gcccggaaca cggaactcaa
      421 ttagcactcg aacgacgaag taaagaacgc gaaaaagcgg aaaaagcagc agagaagaaa
      481 cgacgacgag aggagcagaa acagaaagat aaacttaaga ttcgaaaact cgccttaaag
      541 ccccgcagtt actggattaa acaagcccaa caagctgtaa acgccttcat cagagaaaga
      601 gaccgcgact taccatgtat ctcgtgcgga acgctcacgt ctgctcagtg ggatgccgga
      661 cattaccgga caactgctgc ggcacctcaa ctccgatttg atgaacgcaa tattcacaag
      721 caatgcgtgg tgtgcaacca gcacaaaagc ggaaatctcg ttccgtatcg cgtcgaactg
      781 attaaccgca tagggcagga agcagtagac gaaatcgaat caaaccatag ccgccatcgc
      841 tggactatcg aagagtgcaa ggcgatcaag gcagagtacc aacagaaact caaagacctg
      901 cgaaatagca gaagtgaggc cgcatgacgt tctcagtaaa aaccattcca gacatgctcg
      961 ttgaagcata cggaaaccag acagaagtag cacgcagact gaaatgtagt cgcggcacgg
     1021 taagaaaata cgttgatgat aaagacggga aaatgcacgc catcgtcaac gacgttctta
     1081 tggttcatcg cggatggagt gaaagagatg cgctattacg aaagaattga tggcagcaaa
     1141 taccgaaata tttgggtagt tggcgatctg cacggatgct acacgaacct gatgaaaaaa
     1201 ctggagacga taggattcga caccaaaaaa gacctgctta tctcggtggg cgatttggtt
     1261 gatcgcggta cagagaacgt agaatgcctg gaattaatca cattcccctg gttcagagct
     1321 gtacgtggaa accatgagca aatgatgatt gatggcttat cagagcgtgg aaacgtcaat
     1381 cactggctgc ttaatggcgg tggctggttc tttaatctcg attacgacaa agaaattctg
     1441 gctaaagctc ttgcccataa agcagatgaa cttccgttaa tcatcgaact ggtgagtaaa
     1501 ggaaaaaaat atgtcatctg ccacgccgat tatccttgtg ataaatacga gtttggaaag
     1561 ccagttgatc atcagcaggt aatctggaac cgcgaacgaa tcagcaactc acaagacggg
     1621 atcgtgaaag aaatcaaagg cgcggacacg ttcatctttg gtcatacgcc agcagtgaaa
     1681 ccactcaaat ttgccaacca gatgtatatc gatactggcg cagtgttctg cggaaacctc
     1741 acattgattc aggtacaggg agaaggcgca tgagactcga aagcgtagct aaatttcatt
     1801 cgccaaaaag cccgatgatg agcgattcac cacgggctac ggcttctgac tctctttctg
     1861 gtactgatgt gatggctgct atggggatgg cgcaatcaca agccggattc ggaatggctg
     1921 cattctgcgg taagcatgaa ctcagccaga acgacaaaca aaaggctatc aactatctga
     1981 tgcaatttgc acacaaggta tcggggaaat accgtggtgt ggcaaagctc gaaggaaata
     2041 ctaaggcaaa ggtactgcaa gtgctcgcaa cattcgctta tgcggattat tgccgtagtg
     2101 ccgctacgcc gggcgcaaga tgcagagatt gccacggtac aggccgtgcg gttgatatag
     2161 ccaaaacaga gcagtggggg atagttgctg agaaagagtg cggaagatgt aaaggcgtcg
     2221 gttattcaag aatgccagca agcgccgcat atcgcgctgt gacgatgcta atcccaaacc
     2281 ttacccaacc cacctggtca cgcactgtta agccgctgta tgacgctctg gttgtgcaat
     2341 gccacaagga agagtcaatc gcagacaaca ttttgaatgc gatcacacgt tagcgccatg
     2401 attgccacgg atggcaacat attaacggca taatattgac tttttgaata actttgggga
     2461 aacttgacac caataatggg cgttttttac atgtcattga tgagtctcaa taacctgccg
     2521 ccgagtagtt tttatgctct gaattgtatt tgtgtagtaa acatgctgac tgcaatgtaa
     2581 tagagttttt ttagcctgta acctcttgac ggcattgaat tgcttttgtt atgagttgta
     2641 agccaatgtt atcatcttgt attggggtgg ttatgaagga tggtgcgctg ctcaggagtt
     2701 cttcactttt tattgcctac atgggatgcc ttggatgggg gagtgcttat ttctatggat
     2761 ggggtacttc tttttactac ggcttcccat ggtggattgt aggtgcaggt gttgatgatg
     2821 ttgccagaag tttatttttt gcagttatcg tcattgctat atttcttatc ggttggggta
     2881 ttggtgttgt attctttttc gcagtgaaaa gaaaacattc tatgcaagag ctaaatgtat
     2941 ttcgccttta ttttgctgtg gaattattgt ttgtgccggc aattattgag ttttctatat
     3001 tgagacagaa gattcaggta cctcttttgc tactgtcagc agcgattgcg ctggcggtta
     3061 caatttcgat aagatcttat gggcgatttt tatcggtatc atgcttctat gataagccat
     3121 ttataaaaaa acattttttt gagattgtga tgattgcttt tgtggcatat tt
//
LOCUS       DAZFJF010000262         3149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFJF010000262 DAZFJF010000000
VERSION     DAZFJF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.264"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..243
                     /locus_tag="ACHKAU_005081"
     CDS             <1..243
                     /locus_tag="ACHKAU_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO5533951.1"
                     /translation="QPLRAWVDEAAKADWKSPADIKAQYRTASILKNRRVVFNIKGNH
                     YRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            246..605
                     /locus_tag="ACHKAU_005082"
     CDS             246..605
                     /locus_tag="ACHKAU_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5533952.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(652..1044)
                     /locus_tag="ACHKAU_005083"
     CDS             complement(652..1044)
                     /locus_tag="ACHKAU_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5533953.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1171..1431
                     /locus_tag="ACHKAU_005084"
     CDS             1171..1431
                     /locus_tag="ACHKAU_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5533954.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1428..1865
                     /locus_tag="ACHKAU_005085"
     CDS             1428..1865
                     /locus_tag="ACHKAU_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5533955.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            1952..2962
                     /locus_tag="ACHKAU_005086"
     CDS             1952..2962
                     /locus_tag="ACHKAU_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO5533956.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            2874..>3149
                     /locus_tag="ACHKAU_005087"
     CDS             2874..>3149
                     /locus_tag="ACHKAU_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5533957.1"
                     /translation="MGEVYSNRWTQKNGAAPSKLWIAQIGAMTEQQIRLVCRQCMDRC
                     RAGETWPPDLAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDG"
BASE COUNT          882 a          672 c          821 g          774 t
ORIGIN      
        1 caaccgctaa gggcatgggt ggatgaggcg gcaaaagctg actggaaaag cccagcagac
       61 attaaggcac aataccgaac ggccagtata ttgaaaaacc ggcgcgtggt attcaacata
      121 aaaggtaatc attaccgttt aattgttgca attgcgtatc agcgaggatg ggtatttgtt
      181 aaattcatcg gaaaccacaa ggaatacgat gctattgatg ctgataccat cgaactggag
      241 taagcatgaa catcaaacct attcgtacag agcaagatta tgaagccgcg ttgcgtgctg
      301 ttgaacccat gttcgacaat gagcccgaaa ttgacacgcc tgagggggat ttttttgagg
      361 tgatgtgttt gctaatagag gaatatgaaa aaaaacatta tcccattgag ccaccatccc
      421 caattgaagc tataagattt cgcatggaac agcaggggct gactgtgaaa gatttggaac
      481 ccgcaattgg gaaaaaaaat cgggtttatg aggtgttgaa tggtaccaga agccttacgt
      541 taccaatgat tcgccgtctt cataatcaat ttggtatccc cctggaaagc ctggttggat
      601 tataaaatct gctagtcatt tgcctgatgc tcgttccaga aaaggaatgc atcaggcagt
      661 tttgtttttc tgccgcagta actcttcaag tttccgttta tagaaatcgc gtttttgctc
      721 catatcacga atgatctgct ctgcgtcgct ttgaggtaac tcatctaaaa gcgatatgat
      781 ttttcgttgt tgttctgtaa gttgcggttg gttgtcatta ctggatacag ccattttatc
      841 gccgagagtt tcttcttcca taaagaacca atggacggga tgttgtgaga gctctgctaa
      901 tttttccagt ttatccattc ttggcatcac gcctttcaac caaccttgca cggattgggg
      961 ttttacacca agacgtcttc ccagctctga ctggtttata ttcaattcct gcaacacctg
     1021 ctgaaggcgt tttacaaaga tcatcaccac ccctcgtaaa ctagttccgc gatcctacag
     1081 aaaaaattga taagtggcat tacaaataga agttgaaatt taaatttaaa tttgtaatta
     1141 tcggtgtcat cgtaaagttc ggagggaaac atgcaaaaaa gtactcaagt gaaaatcctg
     1201 tcaataatga gccaatcaga attagggcgt cgtcttggta aaacaccgca aaccataagt
     1261 gggtggttta aaaaacgagt gccagcggag gaggttatac cagcatgtga ggcgcttgac
     1321 tggggagtaa ctccgcatga attgcgccct gataaatacc ctaatccaac cgatggttta
     1381 cctgttgagt atcaggctaa cgcacaagca gcggcgggag ttgattcatg aaaatcaagc
     1441 atgagcacat ccgcatggcg atgaatgcgt ggctgcttta tccgagggta gggcgcaaaa
     1501 aaatcgctga tgatatagcg acagcatatt ttgagcttga aatgacttat ccaccaatgc
     1561 atgacacctc tacgacagag ggtattggat tgaacataca aaatattttt cgctggcttg
     1621 aaaaggatac gcctgatgct gttgaaaaaa ttcaggcact aattccagct atcctgactg
     1681 ttcttccgcg tgaactgcgt tatcacctca gtatttttga cactgttgag cgccgtgcat
     1741 tactggcggc ccaggaagcg ttgagcacgg caattgatgc gcatgatgat gcagtccagg
     1801 cagtttaccg gaaagcgtat ttcagcgacg gcgggtcatc cggcgagtct gttgtggtgc
     1861 attgatattt atgccagacc cctgctgatt ctgttgattg gggaatcaca gaatatcacc
     1921 agaggatggt tcgtcacaag atgaggcaat tatggccgca ttaccataca tgcaactgta
     1981 catagctgat tacctggctg acaccatgca tttgtccgca gaggagcacg gtgcgtattt
     2041 gttgctgatg ttcaattact ggcaaacagg gaaaccaata cctaaaaaca ggctggcaaa
     2101 aatttcccgt ctgactaacg agcgatgggc tgatgtggaa ccatccttgc gggagttttt
     2161 ttgcgataac agcgatgaat gggtgcatct tcggattgag gaagatctgg catcagtcag
     2221 ggaaaaattg accaaaaaat cagccgccgg aaaagcatct gttcaggcca gaagaagcag
     2281 aaaggaagca tatgttcaaa caaaacaaga gagagattta acaggtgttc aaacaaatgt
     2341 tggtgttgtg tttgaacatg acgcaaacac aaaagcaact aataaagata cagatctaaa
     2401 agaattaaac cccacacata acgtgcgtga acgcgagagt attccgacca gtgagtcgca
     2461 tggtgcgccg ttgcagacag ccgaacctga atacctggac ggcctgagcg aaccgatcgg
     2521 gaaattttcg atgactactg tctggcagcc gtcgccggat tttcgacaac gggcagcagt
     2581 gtggggtatg gctctgcctg agccggaatt tacacctgct gagcttgccg cattccggga
     2641 ttactggatg gcggagggga aggttttcac gcaggttcag tgggagcaga aatttgcccg
     2701 ccacgtgcag cacgtcaggg cacaggtaaa accagtcagc aagggggtaa gccatgcagc
     2761 atcaggtggc acggcatcac gggcagttca ggaaatccgg gcagcacgcg aacagtggga
     2821 acgtgaaaac ggatttatca gcaacggaaa cggcctggaa gctgtgggag cttatggggg
     2881 aggtgtattc gaaccgctgg actcagaaga acggggccgc accttcgaag ctctggattg
     2941 cccagattgg tgcgatgact gaacagcaaa tccgtctggt ctgccgtcag tgcatggacc
     3001 gctgccgggc gggtgaaacg tggcccccgg acctggctga gtttgttgcg ctgatttcgg
     3061 agagtggggc aaatccattt ggtcttacgg tggatgccgt gatggaagag taccggcgct
     3121 ggcgcaatga atcctggcga tacgacggg
//
LOCUS       DAZFJF010000263         3100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFJF010000263 DAZFJF010000000
VERSION     DAZFJF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.265"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..239)
                     /locus_tag="ACHKAU_005088"
     CDS             complement(<1..239)
                     /locus_tag="ACHKAU_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533958.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLR"
     gene            657..1346
                     /gene="paoA"
                     /locus_tag="ACHKAU_005089"
     CDS             657..1346
                     /gene="paoA"
                     /locus_tag="ACHKAU_005089"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO5533959.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTELLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            1343..2299
                     /gene="paoB"
                     /locus_tag="ACHKAU_005090"
     CDS             1343..2299
                     /gene="paoB"
                     /locus_tag="ACHKAU_005090"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO5533960.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            2296..>3100
                     /gene="paoC"
                     /locus_tag="ACHKAU_005091"
     CDS             2296..>3100
                     /gene="paoC"
                     /locus_tag="ACHKAU_005091"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO5533961.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKV"
BASE COUNT          743 a          883 c          830 g          644 t
ORIGIN      
        1 tctcgaagaa aaatatacgg tggattgaga aaattacgcg agcaatcaat ttggcctgca
       61 gccctgatta atgattccgg gccacacgct gcattaaaca tatccaccgg gctacgtggc
      121 ggcaatggaa cttcagcccc ccacttcacg aatgcggaaa caacgccagc aatcagccca
      181 atgaatgcag caagaccata acgtctgcgg ttcggtggag tttgttcaaa tatattcata
      241 tctaccctgc ttgtaccatt atgttataca cctcttcagg agtattcata aaacaaggca
      301 aatgtaaaga actgtattgt tttgtataac aagatagttt cctaatcgcc aatgaatata
      361 agctccatca tttctcccta tttttatatt agaagtgaca gagatttgca gggtgatgca
      421 gagctgaaaa tcacaggttt ccttattggt ttttgcatcg tacaactaaa gcaataaacc
      481 agctcccatc atcatcaaca tcccggcgaa catgaattta ttagccgggc tcatagctgc
      541 attcgcgttt taagaataat cctcctgctg tcgccgacta tgcttaacgt ttaaaaaagc
      601 atcagcactc tcgcaacgca ctcttatttt cccctttaga ataccggagg cctggtatga
      661 gcaaccaagg cgaatacccc gaagataatc gggttgggaa gcacgagccg cacgatttaa
      721 gtttgacccg tcgcgatctg attaaagtga gcgccgcaac agcggcgacc gccgtggttt
      781 atcctcattc tacgctggcg gcaagcgttc cggcagctac acccgcgcca gagataatgc
      841 ccctgacact gaaggtgaac ggcaaaaccg agctgcttga ggtggatacc cgaaccacgc
      901 tactggacgc tttgcgtgaa aatctgcatt tgatcggtac caagaaaggt tgcgatcacg
      961 gacagtgcgg agcctgtacc gtgctggtca atggtcgcag gcttaatgcc tgcctgacgc
     1021 ttgcagtcat gcatcagggg gccgagatca ccaccattga aggcctgggt tcgccagata
     1081 atcttcaccc catgcaggcg gcctttatca agcatgatgg cttccagtgc ggctactgca
     1141 cctccgggca aatttgctca tcagtagcgg tgctaaaaga gattcaggac ggcattccca
     1201 gtcacgtcac ggtcgatttg gtttccgctc cagaaacaac tgccgatgag atccgtgaac
     1261 gtatgagcgg caacatctgt cgctgtggtg catacgctaa catccttgcc gccattgaag
     1321 atgctgcggg ggagataaaa tcatgaaggc gtttacctat gaacgagtga acaccccagc
     1381 cgaggcggca cttagcgctc agcgcgtacc cggcgcaaaa tttatcgcgg gcgggaccaa
     1441 tctgctggac ctgatgaagc tggaaattga aacgcccacc caccttatcg atgtgaacgg
     1501 cctcgggctc gataagattg aagtgaccga cgcgggtggg ctgcgcatcg gcgcactggt
     1561 acggaacacc gacctggcgg ctcacgagcg cgtgcgtcgt gattacgcgg tactctcccg
     1621 cgccctgctc gctggcgcat ctggtcagtt acgtaatcag gcaaccaccg caggtaatct
     1681 gctccagcgc acgcgctgcc cctattttta cgacaccaat cagccctgca ataagcgcct
     1741 gcccgggagc ggctgcgcgg cgcttgaagg ctttagccgt cagcacgcgg tggtaggcgt
     1801 aagcgaagcc tgcattgcca cccatccgag cgatatggcg gtcgcaatgc ggttgctgga
     1861 tgcggtggtg gaaaccatca cgccggaggg aaagactcgc agtatcacac tggctgattt
     1921 ttatcaccct ccgggaaaaa cgccgcacat tgaaaccgcc ctgcttcccg gtgagcttat
     1981 cgttgcggtg acgttacctc cgccgctcgg cggaaaacat atctaccgta aggtgcgcga
     2041 tcgcgcctcc tacgcctttg ccctggtatc ggtcgcggcg attattcacc ctgacggcag
     2101 cgggcgcgtc gcgctgggcg gagtagcaca taagccctgg cgcattgagg ctgcggatgc
     2161 tcagctatcc cagggggcgc aggccgtata tgacacgctg ttcgccagcg cccatcccac
     2221 cgctgaaaac acctttaaac tcctgttggc gaagcgaacg cttgcctccg tactggctga
     2281 agcgagggca caggcatgaa atttgataaa cccgcagggg aaaacccgat cgatcagctg
     2341 aaggttgtcg gtcgtcccca tgaccgcatc gacggaccgc tgaaaactac cggcacggca
     2401 cgctacgcct acgaatggca tgaagaagcc cccaacgccg cctatggcta tatcgtcggt
     2461 tccgccattg ccaaaggacg cctcaccgcc cttgatacgg acgccgcgca aaaagcgccg
     2521 ggcgtactgg ctgtcattac cgccagtaac gccggggcac tcggcaaagg cgacaaaaac
     2581 accgccagac tgttaggcgg ccccactatt gagcactatc atcaggccat tgcgctggta
     2641 gtggccgaga ccttcgaaca ggcgcgagcg gcggcctcgc tggtgcaggc acactatcgc
     2701 cgtaataaag gagcttactc cctggcggac gaaaaacagg ccgtcaatca gccgccggaa
     2761 gacacgcccg acaaaaacgt cggtgacttt gacggggctt tcacctccgc tgcggtgaag
     2821 attgatgcta cctacacgac cccggaccag agccatatgg cgatggagcc gcatgcctcg
     2881 atggccgtct gggatggaaa taagcttacc ctctggacct caaatcagat gattgactgg
     2941 tgccgcaccg atctggcaaa aacgctgaaa gttcccgtgg agaatgtgcg tattatctcc
     3001 ccgtatatcg gcggagggtt tggcggcaag ctgttcctga gaagcgatgc gctgctggcg
     3061 gccctcgccg cccgagcggt gaaacgtccg gttaaagtga
//
LOCUS       DAZFJF010000264         3071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFJF010000264 DAZFJF010000000
VERSION     DAZFJF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.266"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(237..839)
                     /locus_tag="ACHKAU_005092"
     CDS             complement(237..839)
                     /locus_tag="ACHKAU_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533962.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(926..1132)
                     /locus_tag="ACHKAU_005093"
     CDS             complement(926..1132)
                     /locus_tag="ACHKAU_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5533963.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1884..2033
                     /locus_tag="ACHKAU_005094"
     CDS             1884..2033
                     /locus_tag="ACHKAU_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO5533964.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            2845..>3071
                     /locus_tag="ACHKAU_005095"
     CDS             2845..>3071
                     /locus_tag="ACHKAU_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5533965.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          794 a          734 c          824 g          719 t
ORIGIN      
        1 atatcggtga tgaactaaag ctcaggagtt gcgtggtggt gatgaatcaa caggcatata
       61 actgataccc gccggtcaga agttccagcc tgcgactttc ctgtctgcgt gcccgcagtc
      121 cggcgttcag gtgcagcgtg tgacagaacg cacggagagc gaaacggcag gcagacaggc
      181 cgttcagtca gccctggcac cctgcccgga taccagcttc cctgaaacaa ctggcatcag
      241 gtgcgcttat cgtcaaccgt tgtgcggtat gccaggagca cgtactgatc catcagatcc
      301 gtcagtgctg cgctctgctt ctgacggttg atgatccatt cctccagctc tttttctgat
      361 ttccgtttca gattctccgt ccagtcgtca aggcagtttc cggtcattaa atcaagccag
      421 caaagccaga ccagtttgag gcgcaactcc gtgccaccaa actgacggaa gaggctgatc
      481 agtttatttg ccagcgaacg ctgcctcaga cgttcacatt cagacaggag aatgccttcc
      541 agcatcggat gatgaaccag cagatgttca ctggtgcata aattcactat cagatcatca
      601 tccaggggcg gcgtggtccg tttgtccgga ccataaatca gccggtgcag actctggggt
      661 acgatggcaa tctccggttt ttcaccattg aatgacaccc acccgggcgt cagattatgg
      721 aagtagtaga tattcggatg acacacgccc caggatacgg cgtgccgggt gaagccggtc
      781 agccatttct ggtagctttc gtggagcagc tcgtaacggg tctgatactg tggtttcatt
      841 ttcatattcc tcacggggga taacaggcag cagaacgccg ccggcaggcc ggagactgcg
      901 tctgctgcag atgaacggtt aagaattacg gacgggactg tgcaatacgc gcctgcagcc
      961 aggcttccac ttcgcttttc agccatcggg agctgcggcc cattttgatg ggcgcaggaa
     1021 agtcaccatc cttgatgagt ttgtaaaacc acttatcggt caggccggtc agttgagtga
     1081 taaatgccat gtcgaccatc tggtcatcca tcagtgaaac tggggtgatc atgagaatgt
     1141 tcctccggat gttgttaaac cagccggtgt ggtcatgaaa acaacgcacc accttcaccg
     1201 gcataacaac cggggagaaa cgtaaaaaat aatcgggaat gtgaccgcca ggagataaac
     1261 ggggaggtta cggtggctga gccgtaccgg tatgccgggt ggttgttacg aaacgatgct
     1321 tccgcacgcg cgcgcgataa agaagggatg attttcgtga ggaagagggg aaaactggcg
     1381 ttctgataat tgcagagtta tttacggggc ggttacgatg gggcagaccg tgtccggtgg
     1441 gcaggcgaca ggtatcaccg atgggataag actgtctgat tggtattata aatatataag
     1501 agagatcaaa cacatgagat caaatacatg agttcatata tgtgaaataa acacggtgag
     1561 tgtgatcagt gggcatgatg agaacgcagt agcaacagac tgcgctatcc ctgatagctg
     1621 gcgtataacc tgcgtatata aaacgtacca gcaacgcacg tgaacagagt gcattaagca
     1681 tccgtcgttg acaccgacct ggtgagtgac agtctgagag accggttaag tggtggtacg
     1741 ggagtaatga gtttggggag ggtaccagaa tgtcagtctg gtacggtatg ctgacagtat
     1801 cacgttaatt gcagcataaa gtgatgagag gctaatggaa gttaaaacca gagcagttcc
     1861 gtcgttgttc atcccgggag acactggaaa aagtgatttc ccacacgcgt tataagctta
     1921 ctccagcgga gatggaagcc ttcaactctg cggtcgatca ccgactggca gaactgacaa
     1981 tgaacaagct ttacgatcgt gttcccgctt cagtctggaa atatgtcatc tgagcgttcg
     2041 gtaatcacaa ctgcatcgcg ctaggtgtta tccgctttgt gattcttatt ggctcttctc
     2101 aggttattaa aagctgaata tgttcctgtt gtcgggaacc aggataatac cgggagcgtt
     2161 aagcggtata gcgacaacaa agaattcgaa atcaaaaggg aatggtgccg gaggcgtggg
     2221 cggtaatagg ctgattttca ggtattaccg gcttacaacg tgtggttgat attgaggggc
     2281 tgtgagtttc gcgtatttgt cagaaaccca gatgagtttt cctgtatggg acgaaataag
     2341 gcagcggcaa tacctggaaa atttaatatg gtatgaagga gaacctgtcg gttacgctta
     2401 atatacacgg tacactgtca gctcagggga tacatgaacc gttatctcac cagctacaca
     2461 gtatacacac actgacgctg acagtagtca gcaggaccac aataatcagc gccagcagta
     2521 tggtaacgat acggtctgta ttccggtagt agccgaaggg gtcgacagaa ccacaatacg
     2581 ggcaacacac tgtattatta gtaatcgcgt ttccacagtc cctgcacttc ttttgcgtat
     2641 caccataact ccctcctcac agccttaccc gtaaccggtt ttttacatta aaaaacaacg
     2701 ctgaaaattc aaccgtcagt ccggagacga ccgttcgggt tatcacagag tgcctgagac
     2761 agtgtcctgc cggaggtcac ttcagggact ctgcgcattt tttacgacgt ggctattccg
     2821 tatgaaccat acggagattt aaccatgacc tacaaataca accccttctg gcagcaacgt
     2881 attcgtgaga cggtgcggca cgcactggat gttcatcccc gtctgacggc attgcgggtt
     2941 gatctgcgtc tcccggatgt accggcagca acggacgcag ctgtgatatc ccgcttcatc
     3001 aatgccctga aagcccgaat cgacgcttac cagaaacgta agcatcggga aggtaaacgc
     3061 gtgcatccca c
//
LOCUS       DAZFJF010000265         3042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFJF010000265 DAZFJF010000000
VERSION     DAZFJF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.267"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            30..77
                     /locus_tag="ACHKAU_005096"
     CDS             30..77
                     /locus_tag="ACHKAU_005096"
                     /inference="COORDINATES: protein motif:HMM:NF018255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5533966.1"
                     /translation="MFPDEPIEFSIEYEN"
     gene            67..957
                     /locus_tag="ACHKAU_005097"
     CDS             67..957
                     /locus_tag="ACHKAU_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO5533967.1"
                     /translation="MKINDFYLPLLSVIGTGRLYITPEGHACHAYFREVSGNGIRFTL
                     TCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEGDVSVDD
                     VQFTMLNKDVYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHSNTIFFDGYI
                     APGWTDYPVTELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNLFIADVQAAD
                     LLCTISNEAVMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCRLIRNNTTIA
                     YGSIVKINEDFYFTVSLVCD"
     gene            988..1503
                     /locus_tag="ACHKAU_005098"
     CDS             988..1503
                     /locus_tag="ACHKAU_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO5533968.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNA
                     MISNSFSNSVV"
     gene            complement(1722..2081)
                     /locus_tag="ACHKAU_005099"
     CDS             complement(1722..2081)
                     /locus_tag="ACHKAU_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO5533969.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNESSKIDKILEINSNRDLLGMFFLSLKEDSVLLNIAFPADKVNITEFIN
                     LMENGYLLKNEVISLLS"
     gene            2332..2943
                     /locus_tag="ACHKAU_005100"
     CDS             2332..2943
                     /locus_tag="ACHKAU_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO5533970.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          967 a          494 c          628 g          953 t
ORIGIN      
        1 agaattatat ttgtgataag ttaaaagaaa tctttcctga tgaacctatt gaattctcaa
       61 ttgaatatga aaattaatga tttttacttg ccattgttgt cagtcatagg aaccggtcgc
      121 ctctatatta cgcctgaagg gcatgcttgc catgcatatt ttcgcgaagt cagcggtaat
      181 ggtatccgct tcacgttaac atgctcaggc tatgaggggc gtttttggat atcagaagaa
      241 cagttcattc agtggtgcca ggagttattc ccgtatagcg aaagccggct gataccggaa
      301 gatatgataa aactcatgat cttatgggta atgcagactg ctttaccaga aggtgatgtg
      361 tctgttgatg atgttcaatt tacgatgcta aataaagatg tttatccggt aatagaaaat
      421 aataatggag aaaacagact taatgtaatt atactcgaaa taacggtaca gagtttacaa
      481 tatctaatta atgaaaattg gcaatttgtt ccacattcta ataccatttt ctttgatgga
      541 tatattgctc ctgggtggac tgattacccg gttacagagc tgactgttgg tgatagttta
      601 aggctctatc atgttgatga cagcaaagaa agagagtgtt ggcttgttat taataaccct
      661 ttagcaaccg taaaactttg tgataataat ttatttattg cggatgtcca ggctgcagat
      721 ttgttatgta ctatcagcaa tgaggctgta atggatcgca tttattgtgt tattggaaca
      781 attcatgttg atatacatat gttgcgcaat gccaaaaaag atgatattat cgattcaact
      841 ggatatcatc tttttggtgg gtgccggtta atacgcaata atacgactat agcatacgga
      901 agcattgtaa aaatcaacga agatttttat tttactgtta gtttagtatg tgattaatca
      961 ggaataagtt atatagggag atgtattatg tcgtcatcgt tatcaggaat aatactcaat
     1021 acttcgctga caagtcacca tatgtcatgg ggcaagttta gcagaactct tcatgtattg
     1081 aatgaagata tcgtagatga aattcagctt aaagcccgaa cacaacaacg ccaccatcga
     1141 gtatattcag aaatggcgga ttactccgtt gtctcctttc ggggcaaaga atattctgtt
     1201 cgttttatta aagattacaa aacagataat tatgtgtaca aagtacaaaa gatgactcag
     1261 gaaagtgggt gtctctcaag attatttagc tttctttctg gcggtgtgac taaggctttg
     1321 gaaagtaaac taaatgagag gcatatcact ccactttctc caacctggtt tcctcgaact
     1381 ccactggagg gggtcttaac ggaaagagga ttatcttctt tgttacgtcg cgtacagtct
     1441 actgaaaacc ttgattatag taatgctatg atatcaaact ctttctctaa ctccgtagtg
     1501 tgattatcat ttgagctttc ctcctggttt aactccagaa taattgtgat tctggagttt
     1561 ttctgtttgt tttatgtcga taatacattg tttttaggaa aatatgggat gaatattcag
     1621 agtgagtttg gctggcagaa gtcagagatt ctgtgatgag gaggggattg agctgaatgg
     1681 atgaattcct tccagctcat aatttatcag gataaaacat cttacgacaa taaactaatt
     1741 acttcattct ttaataaata accattttcc attaaattga taaactctgt aatgttaacc
     1801 ttatctgcag ggaatgcaat attcaaaagt acgctatctt cttttaatga tagaaaaaac
     1861 ataccgagta gatctcgatt gctatttatt tctagtattt tatcaatttt tgaagattca
     1921 ttattgaata aataacaata aaaaaacagg acctcagatt gaggtgcaca ctccagtatc
     1981 cattcatgac catcgtgatc atggaaagat agggcgttag tttgttcttc aagagttatt
     2041 ccgaacgtat gtgcaagatc tgtgcgaaat tgttcattca tattgtagtc ctgtcattct
     2101 ttcaatgagg gtatgcttaa acgtaattaa ctcgtgagct ctggcacaaa taataagacg
     2161 aaaatataag atgcaagtaa tagtgttgca tatttatgat agcatgtgat aaatgtagtg
     2221 caactacttg tagatagtgc cggaggaaaa tttaaatata aaacaattat tgtttttaat
     2281 gaaaataaaa aacattttgt tatcgttata taatatataa aggtttattg tatgtttagt
     2341 ccaatgacaa tggtaggtag atcgttagct caggcggcta cacaaactct tagacctgcg
     2401 gtcaccagag ctgcgacaca agcgggggtg ggagccagtg gcatgaagtt cgtgcccatg
     2461 cagtcgaact ttatgattaa tcatggcaaa ctgactaacc agctacttca ggccgtggcc
     2521 aaacaaaccc gtagcggtga tactcagcaa tggttcaaac aggagcaaat tacttatata
     2581 tccagaacag taaacagagc tattgatgac tattgcatga gtaataattc ggtaattggt
     2641 aaggaaacca aaagccacat tttcaaggct gttgaaagcg cgataaagca gcctctcgat
     2701 atgaattgtg cacaaacgtc gattggccat ttcttacagt ccaacaaata ctttaataaa
     2761 aaagttgatg agcagtgtgg caaaggggtg gatccaatta cacgctttaa cacccagacc
     2821 aggatgatag aacaagtctc acaagaaatc tttgaacaga aatttggtgg tttgaaagtt
     2881 agtgatatca aagctctgac tcagagcacg attgcacagc atgtacagga tactcggttg
     2941 taggaataac ctgttatata tgcgtgttgg gcataagtta agcttcctgg tgtatagcgt
     3001 agtctttgct gatgtaagaa aaggctatat acggaaactt ga
//
LOCUS       DAZFJF010000266         3038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFJF010000266 DAZFJF010000000
VERSION     DAZFJF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.268"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..981
                     /gene="nleC"
                     /locus_tag="ACHKAU_005101"
     CDS             <1..981
                     /gene="nleC"
                     /locus_tag="ACHKAU_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO5533971.1"
                     /translation="IPVLQPGFNFFAPAGYSAAVAPNRAENAYADYVLDIGKRIPLSA
                     ADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDAVSYGIHNEEVH
                     IGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEAGENENEEPYVS
                     FSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEILARRVAQELGWS
                     VPDFKGYAEPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISDRYEASPDFTEY
                     SAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVDRHN"
     gene            complement(1102..1185)
                     /locus_tag="ACHKAU_005102"
                     /pseudo
     CDS             complement(1102..1185)
                     /locus_tag="ACHKAU_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            1270..1341
                     /locus_tag="ACHKAU_005103"
                     /pseudo
     CDS             1270..1341
                     /locus_tag="ACHKAU_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(1352..1594)
                     /locus_tag="ACHKAU_005104"
     CDS             complement(1352..1594)
                     /locus_tag="ACHKAU_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5533972.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            1726..3009
                     /gene="ydfJ"
                     /locus_tag="ACHKAU_005105"
                     /pseudo
     CDS             1726..3009
                     /gene="ydfJ"
                     /locus_tag="ACHKAU_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT          805 a          634 c          748 g          851 t
ORIGIN      
        1 attcccgttc tccaacctgg cttcaacttt tttgcccctg ctggatactc tgctgccgtt
       61 gctcctaatc gtgctgaaaa tgcctatgcg gattacgttt tggatatagg taagcgaata
      121 ccgctttccg cagcagattt aagcaacgta tacgaaagtg taattcgcgc cgtccatgac
      181 agccgtagca ggctcatcga tcagcatacg gtcgatatga ttggcaacac tgtacttgat
      241 gcgttgagcc gatcacaaac atttcgtgat gccgtaagct atggcattca taatgaggag
      301 gtacacattg gttgcattaa atacagaaac gaatacgagc ttaacggaga atctgctatc
      361 aaaattgatg atattcaatc actaacctgt aacgaattat atggatacga tgtcgggcaa
      421 gaaccaattc tccccatttg cgaggcagga gaaaacgaga acgaagagcc ttatgtcagt
      481 tttagtgttg cgccagatac tgattcttat gagatgccat cgtggcagga aggactgatt
      541 cacgagatta ttcatcatgt tactggggcc agcgatccat ctggagatag taatatagag
      601 ctaggaccca ccgagattct cgcacgtcgt gtcgctcaag agctgggatg gagtgttccc
      661 gacttcaaag gatatgcaga gccagaacga gaagctcatc ttagactacg taacctgaat
      721 gcccttcgac aggctgccat gaggcatgaa aagaatgaga gggctttctt cgaaagactg
      781 ggtacgatca gtgaccgata tgaggcgagt cctgatttca cagagtattc cgctgtgtct
      841 aacataggat acggatttat ccagcaacat gattttccag gggtggctat cgacgagaat
      901 ttacaggatg taaatcagat ccaactgtat catggtgctc cttatatctt tacatttggg
      961 gatgtggaca gacacaatta gcgctgattc gtctttacag ttacataggg ttactactca
     1021 tagatattaa cggagttgat gatgggcaat cgtgcaacat tgtattgtat gtaaaggcag
     1081 agctccgaat tttactgttg taagtgggcc tggcgggaat ggtatcgcca gcatcattgg
     1141 cttgggtcca gtaaaaagag acgcagaaag atgcacaata ggcactacgc gtcatgcatg
     1201 gattaggtcg ctcatcattt acacagtact gctaaaaaat tattaaggaa agcgcggcac
     1261 ttcgtatgta agagcgtgtc gcggttggct ggtgaacttt cgatagtgcg agtattgaat
     1321 gatttccagc cgttaccgat ttacgtgttt attagcgaac aaaccactcg tcagcagact
     1381 cccaggtatc tttcagcgtc tcctgaacaa aagtttttgc agaatcttta tcggcggtgc
     1441 gagtaactga aaggccatcg ttgctggtgg atttgatgat cacttctaca tcgtcataac
     1501 gtttactgac acgtcgcagc atttcttgtt gcagggcagg aacagaacct tttggcattt
     1561 tgttgatttt ggctgcgggg tatctgtggg gcatatatgg gacataaaaa ggcctcaaat
     1621 aaacagcaaa atcgagatta gggattttta gaaaaaatac aaccatctga aaaaacttag
     1681 aaaacaccca aaaacccaca gaatagtaaa aaacatctat gaattatgga tttccagtta
     1741 tattcgctcg gcgcagcgtt agtgtttcat gaaatatttt ttcctgaatc atcaacggca
     1801 atggcgttaa ttttggcaat gggaacctac ggtgcaggtt atgtggcgcg tattgtcgga
     1861 gcatttattt tcggcaaaat gggcgacaga atagggcgta aaaaagtgct ctttattacc
     1921 atcaccatga tggggatctg taccacctta attggtgtgt taccgaccta tgcacagatt
     1981 ggtgtttttg cccccatctt gctggtgacg ctgcgtatta ttcaggggtt gggtgcaggt
     2041 gcggaaattt ccggtgccgg tacgatgctg gcggaatatg cgccaaaagg taagcgcgga
     2101 attatctcct catttgtagc tatgggaact aactgcggaa ccttgagcgc aacggcaatc
     2161 tgggccttta tgttcttcat tctcagtaaa gaggaactgc ttgcgtgggg atggcgtata
     2221 ccgttcctgg cgagcgttgt cgtgatggtc tttgctatct ggttgcgtat gaatctgaaa
     2281 gaaagcccgg tctttgagaa ggttaacgac agcaaccaac ccacagcaaa acctgcacct
     2341 gctggtagca tgttccagag caaatccttc tggctggcaa cagggctgcg ttttggtcag
     2401 gcgggtaact ccgggttaat tcagactttc cttgcaggct atttagtgca gacgttattg
     2461 tttaacaaag caattccaac agatgcattg atgattagtt cgattctcgg ctttatgacc
     2521 attccgttcc ttggttggtt atccgataaa attggtcgcc ggatcccgta tattattatg
     2581 aatacctccg cgattgtgct ggcatggcca atgctttcta tcattgtaga taaaagctat
     2641 gccccgagca ccattatggt tgcactgatt gtgattcata actgtgcggt gctgggatta
     2701 tttgctctgg aaaacattac catggcagaa atgttcggct gtaaaaaccg ctttacccgg
     2761 atggctattt ctaaagaaat tggtggtctt atcgcttccg gttttggtcc tatcctggcg
     2821 ggtattttct gcaccatgac ggaatcctgg tatccgatcg ccattatgat catggcatat
     2881 tcagtaattg gtttaatctc tgcgctgaaa atgccagagg tgaaagaccg tgatttaagt
     2941 gcgctggaag acgctgcgga agatcaaccg cgtgttgtaa gagctgcgca accttccaga
     3001 agtctgtaaa tcctcaatcc cttctcttac cggagagg
//
LOCUS       DAZFJF010000267         2941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFJF010000267 DAZFJF010000000
VERSION     DAZFJF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.269"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1520
                     /gene="ydiF"
                     /locus_tag="ACHKAU_005106"
                     /pseudo
     CDS             1..1520
                     /gene="ydiF"
                     /locus_tag="ACHKAU_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            1534..2685
                     /gene="ydiO"
                     /locus_tag="ACHKAU_005107"
     CDS             1534..2685
                     /gene="ydiO"
                     /locus_tag="ACHKAU_005107"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO5533973.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRSERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2728..>2941)
                     /locus_tag="ACHKAU_005108"
     CDS             complement(2728..>2941)
                     /locus_tag="ACHKAU_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5533974.1"
                     /translation="MTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSD
                     YRRQFKNCFAEQEILSEFPQPVSLVG"
BASE COUNT          781 a          681 c          759 g          720 t
ORIGIN      
        1 cccgacgaag caacactttg tgtgttaggc gctggcggcg gtattctgga agccaccacg
       61 ttaattacag ctcttgctga taaatataaa cagactcaaa cgccacgtaa tttatcgatt
      121 attagtccga cagggcttgg cgatcgcgcc gaccgtggta ttagtcctct ggcgcaagaa
      181 ggtctggtga aatgggcatt atgtggtcac tggggacaat cgccgcgtat ttctgaactc
      241 gcagaacaaa ataaaattat tgcttataac tatccacaag gtgtacttac acaaacctta
      301 cgcgccgccg cagcccacca gcctggtatt attagtgata ttggcatcgg gacatttgtc
      361 gatccacgcc agcaaggcgg caaactgaat gaagtcacga aagaagacct gattaaactg
      421 gtcgagtttg ataacaaaga atatctctat tacaaagcga ttgcgccaga tatcgccttc
      481 attcgcgcta ccacctgcga cagtgaaggc tacgccactt ttgaagatga ggtgatgtat
      541 ctcgacgcat tggttattgc ccaggcggtg cacaataacg gcggtattgt gatgatgcag
      601 gtgcagaaaa tggttaagaa agccacgctg catcctaaat ctgtccgtat tccgggttat
      661 ctggtggata ttgtggtggt cgatccggat caaagccaac tgtatggcgg tgcgccggtt
      721 aaccgtttta tttctggtga cttcaccctt gatgacagca caaacttagc ctgcccctaa
      781 accaacgtaa attagttgcg cggcgtgcat tattcgaaat gcgtaaaggc gcggtgggga
      841 atgtcggcgt cggtattgct gacggtattg gcctggtcgc ccgggaagaa ggttgtgctg
      901 atgactttat tctgacggta gaaacaggtc cgattggcgg aattacttca cagggtatcg
      961 cctttggcgc gaacgtgaat acccgcgcca ttctggatat gacgtcccag tttgattttt
     1021 atcacggtgg tggtctggat gtttgttatt tgagttttgc tgaagtcgac cagcacggta
     1081 acgtcggcgt gcataaattc aatggcaaaa tcatgggtac cggtggattt attgatatca
     1141 gtgccacctc gaagaaaatt attttctgcg gcacgttaac tgcgggcagt ttaaaaacag
     1201 aaattgccga tggcaaattg catatcgtcc aggaaggacg ggtaaataaa tttattcggg
     1261 aactaccgga aattactttt agcggaaaaa tcgcgctcga acggggactg gatgttcgtt
     1321 acatcaccga acgtgctgta ttcacgctga aagaagacgg cctgcattta atcgaaatcg
     1381 cccctggcgt cgatttacaa aaagatattc tcgacaaaat ggacttcacc ccagtgattt
     1441 cgccagaact caaactgatg gacgaaagat tatttatcga tgcggcgatg ggttttgtcc
     1501 tgcctgaagc ggctcattaa caggagtata atgatggatt tttctttaac tgaagaacaa
     1561 gaactgctgc tggccagtat tcgcgaactg attacgacta actttccgga agagtatttc
     1621 cgcacctgcg atcaaaacgg gacatatccg cgtgagttta tgcgggcgct ggcggataac
     1681 ggtatttcca tgcttggcgt gccggaagaa tttggtggta tccctgcgga ttacgtcacc
     1741 caaatgctgg cgctgatgga agtgtcaaaa tgcggtgctc cggcattttt aattaccaac
     1801 ggtcaatgta ttcacagtat gcgccgtttc ggttctgcag agcagctacg taaaacggca
     1861 gagagcaccc tggaaacggg tgatcccgcc tatgccctgg cgttgacgga accaggtgct
     1921 ggctcagata acaacagtgc cactaccact tacacgcgta aaaacggcaa ggtctacatc
     1981 aacggacaaa aaacctttat taccggtgcg aaagagtacc cgtatatgct ggtgctggcg
     2041 cgcgatccgc aaccgaaaga tcccaaaaaa gccttcaccc tgtggtgggt cgactccagt
     2101 aagcccggca ttaagattaa cccgctgcat aaaatcggct ggcatatgct cagcacctgc
     2161 gaagtctatc tcgacaatgt ggaagttgaa gagagcgaca tggtgggcga agaaggaatg
     2221 ggtttcctca atgtgatgta caactttgag atggagcgcc tgatcaacgc cgcgcgcagc
     2281 accggctttg ccgaatgcgc ctttgaagat gccgcccgct atgccaacca acgtatcgct
     2341 tttggtaagc ccattggtca taaccagatg atccaggaaa aactggcgct gatggcgatt
     2401 aagatcgaca acatgcgcaa catggtgctg aaagtggcat ggcaagccga tcagcatcag
     2461 tcactgcgca ccagcgcggc gctggcaaaa ctgtattgcg cacgtaccgc aatggaagtc
     2521 attgatgatg cgattcaaat catgggcggt ctgggctata ccgatgaagc gcgcgtctcc
     2581 cgcttctggc gtgatgtccg tagtgaacgt atcggcggcg gtacagacga aattatgatt
     2641 tacgtagcag gtcggcagat cctgaaagac tatcagaaca aataatctgc aggcggcgca
     2701 gcttcttaac aaactgcgcc gccagattta tccaacaaga cttaccggtt gaggaaattc
     2761 agataggatt tcttgttccg caaaacagtt tttaaattgt ctacggtaat cgctgggcga
     2821 acaaccgaca tggcgcaaaa acagtttggc aaagtgatcg acattttcat aacccacacg
     2881 ccaggagatc tctgcctgtg ataattcagt attagtgagt gaccattttg cttccgtcat
     2941 a
//
LOCUS       DAZFJF010000268         2937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFJF010000268 DAZFJF010000000
VERSION     DAZFJF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.270"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1259
                     /gene="iha"
                     /locus_tag="ACHKAU_005109"
     CDS             <1..1259
                     /gene="iha"
                     /locus_tag="ACHKAU_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO5533975.1"
                     /translation="TLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRNVLK
                     RDKWGLAGQLRELKESNLILNSLLLTPLGESHLVTVGGEYQSSSMKDGVVLASTGETF
                     RQKSWSVFAEDEWHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVT
                     TGYKAPRMGQLHKGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANITG
                     FMTDFSNKIVSYSINDNTNSYINSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQ
                     RDGDNKGAPLSYTPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKK
                     VYDEKGEYLKAWTVVDAGLSWKVTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGD
                     YFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1513..1870)
                     /locus_tag="ACHKAU_005110"
                     /pseudo
     CDS             complement(1513..1870)
                     /locus_tag="ACHKAU_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2090..2854
                     /locus_tag="ACHKAU_005111"
                     /pseudo
     CDS             2090..2854
                     /locus_tag="ACHKAU_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          719 a          751 c          760 g          707 t
ORIGIN      
        1 ggaccctgcg ctatgagcga aacaaaattt cagctggcta tgatcatact ttcaccttcg
       61 gaacatggaa atcgtatctg aactggaacg agacggaaaa taaaggtcgt gagcttgtac
      121 gcaatgtact gaaacgcgac aaatggggcc ttgccggtca actgcgggag cttaaggaat
      181 ctaaccttat cctgaattca ttgctgctta cccctctggg agaatctcat ctggttactg
      241 tggggggcga gtatcagagt tcgtccatga aagatggggt tgtccttgcc agcacaggtg
      301 aaactttccg gcagaaaagc tggtcggtat ttgctgagga cgagtggcat ctcacggatg
      361 cacttgcgct gactgcgggc agccgctatg aacatcatga gcaattcggg gggcacttca
      421 gtccgcgtgc atatctggtc tgggatgtgg cagatgcctg gacgctgaaa ggcggtgtga
      481 ccacgggata taaggcaccc agaatggggc agctccataa agggattagt ggtgtgtccg
      541 ggcagggaaa aacaaatcta cttggtaacc ccgacctgaa gccggaagag agcgtcagtt
      601 atgaggctgg ggtgtattac gataaccccg ccggtctgaa cgccaatatc acaggtttta
      661 tgactgactt ctctaacaag attgtctctt attccataaa tgataacacc aatagctata
      721 taaacagcgg aaaggcccgg ttgcacggtg tggaatttgc cggcacattg ccgctatggt
      781 cagaggatgt cacgctgtcg ctgaattaca cctggacccg aagtgaacaa cgtgatggtg
      841 ataataaagg tgcaccgctg agttataccc cagaacacat ggtgaatgcg aaactgaact
      901 ggcagatcac cgaagaggtg gcatcatggc tgggtgcccg ttatcgcggg aaaacaccac
      961 gtttcaccca gaattattcg tcgctgagcg ctgtacagaa gaaagtatat gatgagaaag
     1021 gagaatacct gaaagcctgg acggtggtgg atgcaggtct gtcgtggaag gtgacggatg
     1081 ccctgacgct gaatgcggca gtgaataacc tgctcaacaa ggattacagt gacgtgagtc
     1141 tgtacagtgc cggtaagagt acgctttatg ccggtgatta cttccagacg ggatcatcaa
     1201 caacgggata tgtgatacct gagcgaaatt actggatgtc gctgaactat cagttctgat
     1261 aacaacaaaa cgctatcact gacggtagca tacgttgcca ctgcaactcc tggcggaacg
     1321 gtggcaatgt tttaggttaa gtgcgtttcc ggtccgctaa tgagatttcg ttaccaacaa
     1381 ctaatatcat caccggaaat gcacggatta ttttttaact catcatttac atacttgtcc
     1441 agaaatttat actcggctag ctctgaatta agggccgcgt ccgcatctcc attaccgtct
     1501 tatatcactg gcgctgacaa ttgtctcaca tatagctgca gtatcggctc catccccgtg
     1561 gctacgcgct gaaattctag tgaaccgctt tcgtggactt gagtgaacca actgtttagc
     1621 tgtgatttat ttggggggca gaatacggta gaactcgagt gccagttgct gcgctatttt
     1681 ctgttccggt tctgtttcac aaatcgggac gcataatttt cttccccctg attattttcc
     1741 agggcaccac cccgcagctt cccagggaca gtgaaggaag tcgggcggca atagcaaacg
     1801 gcgggatcca acctttacgc catttggcaa cagtatctct gatgatagtt tcagtgcctg
     1861 taactccccg ttactttatt cgtacccttg taatggggtg ttagccagcc ccggcatgat
     1921 taattccccc agtcgtccat gattcggggg gatgtcaccg ggtctggtgg ggcgctggta
     1981 accgctgata ggggcctggt cagacatcat gtcgggacag tctgtaacgt ggatgccggt
     2041 acctgctccc caaggttatc tggttaaccc gtataaacgg ggagacacaa tgcccgaatc
     2101 cagcgattac gaatcaatcc tggtctttat tggcgttgat gtaggtaaag acacccatta
     2161 cgccgttgcc gttaatcgtt caggtaaacg tttgttcgac agagtattac ccaacgacga
     2221 aaacaaactc agagcgctga tatctgacct gaaacagcat ggacagatac tgctggtcgt
     2281 tgaccagcct gctaccatca gggcgttacc cgtcgccgtt gcccgctctg aaggcgtcct
     2341 tgtcggatac cttcctggac tggcaatgcg acgcatcgcc gatttgcact ccggtgaagc
     2401 caaaaccgat gcccgtgatg cggccatcat tgccgaagct gcacgtactc tgcctcatgc
     2461 tcttcgctct ctaaaactgg ccgacgaaca aatcgccgaa ctctccatgc tttgcggctt
     2521 cgatgatgat cttgccgcac agacaacaca ggccagcaac cgcatccgcg gcttgttgac
     2581 ccagatacat ccggctctgg agcgcgttct cggcccgcga ctggatcatc ctgcagtact
     2641 tgatcttctc cagcgatatc cctcacccga aaaactcgct ttactgggtg agaaaaaact
     2701 ggcaacccag ctctgcaaac tcgcgccccg tctgggaaaa cgccttgctg cagatatccc
     2761 ccaggcactg acacagcaaa ccgtcatcgt ccccggtacg aatgctgctg tcgttgttct
     2821 cccacgcctg gcactccagc tcatcacgct gcgttagcca agagacgaag tgactctttg
     2881 aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc agatcat
//
LOCUS       DAZFJF010000269         2907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFJF010000269 DAZFJF010000000
VERSION     DAZFJF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.271"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..547)
                     /locus_tag="ACHKAU_005112"
     CDS             complement(<1..547)
                     /locus_tag="ACHKAU_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5533976.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRALFLMH
                     YGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
                     TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHM
                     SESLLKIKLKNEGHMFSRLLLE"
     gene            complement(685..882)
                     /locus_tag="ACHKAU_005113"
     CDS             complement(685..882)
                     /locus_tag="ACHKAU_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5533977.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(1169..1987)
                     /locus_tag="ACHKAU_005114"
     CDS             complement(1169..1987)
                     /locus_tag="ACHKAU_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO5533978.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(2139..2510)
                     /locus_tag="ACHKAU_005115"
     CDS             complement(2139..2510)
                     /locus_tag="ACHKAU_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5533979.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(2500..2871)
                     /locus_tag="ACHKAU_005116"
     CDS             complement(2500..2871)
                     /locus_tag="ACHKAU_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5533980.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
BASE COUNT          962 a          718 c          501 g          726 t
ORIGIN      
        1 cttccagcaa caagcgactg aacatgtgcc cttcattttt cagttttatc tttaacaaac
       61 tctcactcat atgcaggcgt aacgcaatcg caccaagcgt ccagcttgct gatatatctg
      121 tctgaattat cgccctgact ttggcactta tactggataa acatccactt aaaaataata
      181 atatccgttc atctgattca aacagcgaca ggcatgccat cataagaaac atatccgtgg
      241 tatctccgga aaatctctgg ctggtaatta aagcctcagc caacgcaggg ttgttgggtt
      301 ccagtgacag ataaagcgga acgtcagtca gatgagttct tgtcagctta tgctgaattt
      361 ccagatattg acttactatg gaatggttta tatcgaaaat tttaactttg ccataatgca
      421 taaggaaaag cgccctgatg catttggtgg ccagaacgac tgagccgggc ttaagtgaca
      481 acgtatcctt ttcaagaaaa atattaattg gggagcaaac catgataact gaacagacaa
      541 caaccattat aattttactt tcattagcaa ttggttagtt caattatagc cccaaaaggt
      601 aaattatcat caacacataa gcaaaggact gacaggtgcc gctaacaccc accagccgcc
      661 catttaccac aaataaaaaa gccttcagga ctgaaggcgt ctgtaacaac cgcactgata
      721 gtctgccaga cccgccataa caagctgggt cagtattaac tggcagcgtt cgcgtgaaag
      781 gtaagtattc tgcgcaatct ccccgactgt cgccggttcg gtgacgctta attcatcaaa
      841 cacaactctg gcggtttctg tcatatcctg ctgtttcagc atgtcttttt accctttccg
      901 gttaacgtga cacaccaata actcttgccg aaaaagccag caagctgaaa gacctgtatt
      961 aataactacc agcacattta acgcactgcg ctactttgcg ggcacaaaaa acccgctcag
     1021 aggcggggtc aagctatgcg gcgaaataac cactcttaac agcatacctg attttttacg
     1081 tacgtaaatg ctttgccgtg catatttttc atgcaaatgt cacgtcctgc tatttttcag
     1141 tcttataaat ttaaaaccat agaaaaaatc aattatgttt taaaaatgga taggtaaaga
     1201 ataacaagtg acacagattc aaaccaaaat ggaaaagggt ggcaacccac aaacgcccac
     1261 tccacatcca tgacaatcca tacacaacac cagataacgt ggcaaataat acaagtaaag
     1321 caccacctga atagtgataa aaaccaaaca acaaagccgc cacaattaat gcaaccaatg
     1381 gagacgttac ttctgatagc cgtgattgaa tataccctct aaataatgat tcctctgcca
     1441 gagacacaaa aaacaaatta gccaatataa actctggcaa ccactcagga aaatgaatct
     1501 ctggctttaa tccaccaaaa aaaacagcca aaaacaggat aagaggaaca gagagcgaca
     1561 gagcccccca cttccacaaa gacacttctg attttacttc ttttttaaac aaagaagatg
     1621 tacacaggac taacaaaaat ggcaccagcg ctttatcaaa attaaaatac attgtatagg
     1681 gagtactctg agggccaaca gtaacagaat ttagcactac aggattgtga aaccctggcc
     1741 atagatggaa agataatgct atggctgata aaactatgcc aacttcatat atagatttag
     1801 cccaggcgtt atatttccag ttgaacttca aaataataaa aaaaacgatt gtagcaacaa
     1861 aaaataacac cgaccaatca ataatatcat taagcacagc caggacaaca gacaccgtca
     1921 acaatgaaaa agccactacc ttatgccaac tgagagttga cagtgacagt actaacacaa
     1981 tccacataag cactcctttt atttaatgat gaagattgat tatcaatatt ttcaattcac
     2041 caggcaacat tttatctacc ttccacaata catgaccatc aaaaaataaa catgttaatt
     2101 ataaacacag aaaacataac cctcatcact atatatccct accgcatatc catatctaac
     2161 cggacattca gagccataag cattccttca ataatacctt ccgctttata aagcctttta
     2221 ccaataagcc catcagaaca tctatgctta cgtgcaagag ccataaatgt cattccacct
     2281 acgtaataat ccaccaacaa atcgtgcaaa tactgattat tcttgtttaa tcgtgccata
     2341 caaccacaaa ttatcatggc gtcatcatca gaacactgag gacgtgattt cactttagtc
     2401 gggattaatc ctttaaaacc agcagctatc gagggccatg ttacatcttc atgattattt
     2461 gctgcccatg ccccccatcg ctcaagaacc atctggatat cacgcgccat cgttaccacc
     2521 tctgatttcg taaatcttca cgcccagccg tccacctggc acaggctgac cgcgcacaat
     2581 attgatttca tcaaactgct cgtcatcaat gagcacttcc gcatgcgtca gcgcatccag
     2641 cggtgctttc agaatgttgt ccaggtcacg gcggcgctta tccggtggtt ctgcaataat
     2701 ttttattgcc agccgtccgg acaggcttaa tttcagccgc tgctggcgaa caataagcgc
     2761 cacagcccgg cgataacgct caccggcttt tgatacaaaa tatgtgctgc cacgacgtcg
     2821 ccagtaggtg ttcaccgtcg gcgggtaagg caaaacaaac tctatacgca tcagtaacct
     2881 cttttacccg agcacgccgg ttgcaaa
//
LOCUS       DAZFJF010000270         2881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFJF010000270 DAZFJF010000000
VERSION     DAZFJF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.272"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>2881)
                     /locus_tag="ACHKAU_005117"
     CDS             complement(<1..>2881)
                     /locus_tag="ACHKAU_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5533981.1"
                     /translation="IQLKAKRNSTTVVVNTLASENPDEAGRYSMDVEYGQYSVILLVE
                     GFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEALRRFELMVEEVARNASAVAQN
                     TAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAGQAATSAQSASSSAGTASTKAT
                     EASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSAATSASTATTKASEAATSARDA
                     AASKEAAKSSETNASTSASSAASSAMAAGNSVKAAKTSETNAKSSETAAEQSASAAAD
                     SETAAASSAREASIKAEEAAVSATAAGKSAESAASSASTATTKAGEATEQASAAARSA
                     SAAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQASAARGSATT
                     ASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGVVQL
                     SSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTGQKGIVQLSSATNSTSESQAAT
                     PKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSTSETLAATPKAVKAANDNANGR
                     VPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITL
                     IGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTLTGFTNFAWVNTSGDTYDI
                     YVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPG
                     APIPWPSDTVPSGYALMQGQAFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSH
                     RDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAA
                     SAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNY"
BASE COUNT          532 a          802 c          767 g          780 t
ORIGIN      
        1 atagttaaat gcgatgtttt tgacggtgtt ttccgcgtta ccagcagcgt taacggtgat
       61 ggtgtgtcca tgtgaaccaa tcgcaacgga gtgcgtatga gcaccaatac cgacagtatg
      121 cgcgtgtgca cctgcagatg ctgctgtgcc ggacagtgaa tggctatgat taccatctgt
      181 actggtattc gctaaccacc ccgtagacat acctactgag ccttgtacac cccaggtatt
      241 ttgacctgag cttgtataac catattgata agtatcttta aaaacactgg ggttaaatcg
      301 acggccatct ctatggctgt gattaccagc tgcattcgtg ctgccactta aactatgggt
      361 atgcgcacca gtgttattcg tggatttagt gccgtaatca aacgacgatg tggttttcgt
      421 ccccaaatcc gtactggatg cgctggcgct gtgggtatgc gatttaatgc cgtcctgttc
      481 ctgagacaat acggcccgac cactggcagg tttgccctta atcgtccagc cacgcatatc
      541 agggatcacg cctgacggat aagccgctgc aagtttcggg tatgcagatt tgtcaaaagc
      601 ctgcccctgc atcagggcat agccagacgg aacggtatct gatggccacg ggattggtgc
      661 gccaggcgga taaaactgct ctgatggcgt atagagtgaa taaactgtac cgtccgttaa
      721 cccttccggc ttattagcag aatatgctgg tgacgtatga atcgtcacgc tggcattact
      781 ggtataatcc cattgaatat ttacaccagt cgcataattt ccgattgcaa cgtaaatatc
      841 gtaagtatca ccagatgtat taacccaggc aaaatttgta aaccctgtca atgtgcgctg
      901 ccataaagca ccagtaatcc ccttcggatt accattacct gcacgtaaaa caagttcaga
      961 tatacctgcc tgttgaggtg accccacgtt aaatccagcg ccaccaatca acgtaattga
     1021 aacaacagaa ctcgcctgtg gcatggttac cgttgctaat ttgaaccaac cagcaccacc
     1081 gctgaatgac attgttgttg agttaagcgt accaatatct ttcggcgtca gtgttatatc
     1141 cgctgaaagc gccttaccat tcaccttacg ggcagaaggt acccgaccat tcgcattgtc
     1201 attagctgct ttcactgctt tcggtgtcgc agcaagcgtt tcagatgtgc tgttggttgc
     1261 actactgagc tggacaattc ctttttgtgc tgtcgtagcg tcctgtgcgg tgtatttccc
     1321 gttagccagg tcatacgcgg ccttaacggc ttttggcgtt gccgcctgtg actcggaagt
     1381 gctgttagtc gcactgctga gctgaactat ccctttctgt cctgtcgttg catcctgtgc
     1441 ggtgtatttc ccgttagcca ggtcatacgc ggccttaacg gcttttggcg ttgccgcctg
     1501 tgactcggaa gtgctgttgg tcgcactgct gagctgtact accccctttt tcgtcgtgct
     1561 cgcatcctca agcgccacgg cggatgcaat atcctctgca cgttttgccg ctgtctcagc
     1621 gcgcgttgct gcggattctg ccgtactttt gctctgtgct gccgctgccg cactgccagc
     1681 agcctctgtc gccttcgtgg atgccgtcgt ggcgctgccc ctcgctgctg acgcctgtct
     1741 ggtcgcctca tcttttgaag cagacgcaga tgatgccgat gacgccgccg aactggcgga
     1801 cgatgcggca gccgtttttg aggattctgc gctggtttcc gacgctttcg cgttcgtttc
     1861 ggatgtcttc gctgcggaag cagacctcgc tgctgcgctg gcctgttcag tggcttcgcc
     1921 agccttcgtt gtggctgttg aagcggatga tgcggcgctt tctgccgatt ttccggcggc
     1981 ggtggcactg actgctgcct cctctgcctt aatggacgcc tcacgggccg atgatgcagc
     2041 tgctgtctca gagtctgccg cagctgaggc gctctgttcc gctgccgttt cagaagactt
     2101 agcattcgtc tcggacgttt ttgccgcctt cacggaattt cctgccgcca ttgccgagga
     2161 ggctgcactg ctggcgcttg tggaggcgtt cgtttctgat gattttgccg cctctttgga
     2221 ggccgccgca tctctggctg aagtggcggc ttctgacgct ttcgtcgtcg cagtggatgc
     2281 agaagtggct gctgattttt gtgacgctgc agcattcgtt tctgacgttt tcgcggcacc
     2341 ggcactggtg gccgccgcgc tttttgagga ctctgcagcg gcagcacttt ttgatgcttc
     2401 tgtggccttt gttgatgctg ttcctgcgct ggaagacgct gactgagccg acgtcgcggc
     2461 ctgcccggct gatgtgctgg ctgcacgtgc tgagcctgca gcatcagtcg catgggttgc
     2521 cgcctcacgg gctgatgtgc cggcatcgcc ggctgacttc ttcgcggctg ccgtgttctg
     2581 tgccaccgcg gacgcgttac gcgccacctc ttccaccatc agctcaaaac ggcgcagtgc
     2641 ctccggacgg gcatcatcct ccgtcatggc accgagaaaa tcattcagcg taccgggtcg
     2701 ggaatcttca tacacggtga tggtcccggc atgtgacggc gggaatcctt ccaccaacag
     2761 aataacgctg tactgaccgt actcaacgtc catactgtaa cgcccggctt catccggatt
     2821 ttctgaggcc agcgtgttca ccaccaccgt ggtgctgtta cgttttgctt tcagctggat
     2881 t
//
LOCUS       DAZFJF010000271         2869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFJF010000271 DAZFJF010000000
VERSION     DAZFJF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.273"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2869
                     /gene="toxB"
                     /locus_tag="ACHKAU_005118"
     CDS             <1..>2869
                     /gene="toxB"
                     /locus_tag="ACHKAU_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO5533982.1"
                     /translation="KLSATGDSLNIMGHLVPVSFIYDIGGVFNGNRISESTDIKNKIK
                     NLKINGDILQHYINTHYLSESQTQKIKDIVDFLGIQDNTKRVKLESDIKHISEVQQPL
                     HSIISRQKEHVKNLLSGLLNEFSNKLRQQGLSLKTNVLSVNNFKASKINSDTVEVTVT
                     DLQGRLYSVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSVIGLVQYTRMIKTNDNI
                     SAIDHAGAVSDIKNIVDKFLGGILTFTNNRVYNPGGISGASLEGFISSGLEVCASRIG
                     GTAGRYLSNVAKVIKLPLLDIGINIWSLYDSSLNHARATTQIEYISTAIDVSFSSINT
                     ALSIGAIAYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVL
                     SINKDTGIIDLSNNQVLGNVHLDMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESG
                     AYNFACTKLSDAGEPDIFGWGDKDVCNSMELSESQLANGYSNRQWPSQSPVIPEGIYN
                     TVYLGYGETLRANTEVTLSSTGYFYEIARAYTDDELSEPLLTVCNQRSSVIGGKEPLT
                     IIIPALEHGMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIIS
                     FEQLSRDFNLDLDLSDERKQNFYFHHPSGFYSGKVMSITQKGINSVVGTKQGYDKIRG
                     NNLDNTFSLGNGGGVIYSGGGSNTYFVPATLQHNLHIYISEKSNGNHIILGETHSRLS
                     IECHFSDDKREFIKMDLYNGGDVILESDTLQKINTFSKNITIQTADGVMANWDDESST
                     LSVYSIDMIAWRDKNKTEKEPLPLDVIQLTNWRIHNSCSLFYDKYQVDIEANKLTYTI
                     LFPDTELPVQLNYASVIYGNHGAKYTFLDSGSKAITIHITDESGDYDTFDFRNIVFEH
                     HTNDMFISFDNQGGFVISISNNATNELTDINVFRKNMTSFDG"
BASE COUNT         1014 a          418 c          543 g          894 t
ORIGIN      
        1 aaattatccg caactggaga ttcattaaat attatgggac atttagtgcc tgtcagtttt
       61 atttatgaca ttggaggagt ctttaatggc aacagaataa gtgaaagcac agatataaaa
      121 aataaaatca agaatctgaa aataaatggg gatattttac aacattatat aaatacgcat
      181 tatttgtcag agtcgcaaac tcagaagata aaagatattg tggatttttt aggcattcag
      241 gacaacacaa aaagagttaa gcttgaaagt gatattaaac atataagtga agttcaacag
      301 ccccttcatt ctattatctc acggcagaaa gaacatgtaa aaaacttact atctggatta
      361 cttaatgagt tttcaaataa gttaagacag caaggccttt cacttaaaac aaatgttctt
      421 tcagttaata acttcaaagc atcaaagata aatagtgata ctgtggaggt tacagtaact
      481 gatctgcaag gaagacttta tagtgttgat atagatacaa gagttattgg cttaactttt
      541 aaagagggga taaatagttt atctgaagca ctcgaacaca tgaatataga tgcaattatg
      601 tctgttattg gcttggttca atatacacga atgataaaaa caaatgacaa tattagcgca
      661 atagatcatg caggagcagt atcagatatt aaaaatattg tagacaaatt tttaggcgga
      721 attttaactt ttactaataa tcgcgtatat aaccctggtg gtatatctgg tgcctcgctt
      781 gaaggattta tatcttctgg tttagaagtg tgcgcgtcaa gaataggtgg caccgctggg
      841 cgttatctta gcaatgtggc aaaagtaata aaattacctt tgctagatat aggcataaat
      901 atttggtcat tatatgactc ttcattaaat catgccagag ccaccacaca aattgaatat
      961 atatcaacag cgatagatgt atctttttca tctataaata ctgctttatc aataggcgca
     1021 atcgcatatc cacctttggc aatagctata gttccgataa ctatcttttc tcatgaagta
     1081 aaaaactatg ctgtttatgt gaatcaaatt aatgagcgac ataagttatg gcttgaagct
     1141 gaaaaatatc tggacaatgg aagcgcaaag gttctaagca ttaacaaaga tacaggcata
     1201 atcgatttat caaataatca ggtgttaggt aatgttcatt tagatatgag agaaaaccct
     1261 ccgatattac atggtgaaaa atcatataat agcgggaaga atattggaag tcatccagat
     1321 atgactgaca gagaaattat ggagtctgga gcatacaatt ttgcatgtac aaaactctca
     1381 gatgcaggtg aacccgatat tttcggttgg ggagataagg atgtatgtaa ttcaatggaa
     1441 ctgtctgaat ctcaattagc taatgggtat tctaaccgcc aatggccttc acaaagtcca
     1501 gttattcctg aagggattta taatacagta tatcttgggt atggagaaac attgcgagct
     1561 aatacagaag ttacattgag tagcactggt tacttttacg agattgcaag ggcatatact
     1621 gatgatgaac tatctgagcc attattaaca gtatgtaacc aacgtagtag tgtaattggt
     1681 ggaaaagaac cattaacaat aatcattcca gctcttgaac atggtatgct tggctctaat
     1741 ctacatatga ttgaacgttt taaaaactac aatttttcaa tatcaggagg gaagggcggg
     1801 ataaaattaa tggttggtgg tattggtgat tacaacatag aatgtacccc cggtgttaga
     1861 aatattattt cttttgaaca attatcacgt gacttcaatc tggatttgga tttatcagat
     1921 gagaggaaac aaaattttta ctttcatcat ccctctggtt tttattcagg aaaagttatg
     1981 agtataacgc aaaagggcat aaattcggtt gttggtacta aacagggata tgataagatt
     2041 cgtggtaata atttagataa taccttttcc cttggaaatg gggggggggt tatatattct
     2101 ggtggaggaa gcaatacata ttttgttcct gctacactac agcataattt gcatatatat
     2161 atatcagaaa aatcaaatgg taaccatatt attttaggtg agacacactc tcgcttatct
     2221 attgaatgcc actttagtga tgataaaaga gagtttataa agatggactt atataatgga
     2281 ggtgatgtaa ttttagaaag tgacacttta caaaaaataa atacattttc aaaaaatata
     2341 acaatacaga cagcggatgg agttatggca aactgggatg atgaatcaag tactctgtct
     2401 gtttattcaa tagatatgat tgcctggagg gataaaaata aaactgaaaa agagccttta
     2461 ccacttgatg tgattcagtt aactaattgg aggatacata actcatgctc tttattttat
     2521 gataaatatc aagttgacat tgaagccaat aaattaacgt atacaatttt atttcctgat
     2581 acggagttac ctgttcaact taattatgct agtgttattt atggtaatca tggggcaaaa
     2641 tatacattcc tcgactcagg aagtaaagct ataaccatcc acattactga tgaaagtggc
     2701 gattatgata cttttgactt ccgcaatatt gtttttgaac atcacaccaa tgacatgttt
     2761 ataagctttg ataatcaagg aggatttgtt atatcaatat caaataatgc cacaaatgaa
     2821 ctgacagata ttaatgtatt ccgaaaaaac atgacttctt ttgatggct
//
LOCUS       DAZFJF010000272         2864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFJF010000272 DAZFJF010000000
VERSION     DAZFJF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.274"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..77)
                     /locus_tag="ACHKAU_005119"
     tRNA            complement(1..77)
                     /locus_tag="ACHKAU_005119"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Asp,seq:gtc)
     gene            complement(210..941)
                     /gene="dnaQ"
                     /locus_tag="ACHKAU_005120"
     CDS             complement(210..941)
                     /gene="dnaQ"
                     /locus_tag="ACHKAU_005120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO5533983.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1006..1473
                     /gene="rnhA"
                     /locus_tag="ACHKAU_005121"
     CDS             1006..1473
                     /gene="rnhA"
                     /locus_tag="ACHKAU_005121"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO5533984.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1470..2192)
                     /locus_tag="ACHKAU_005122"
     CDS             complement(1470..2192)
                     /locus_tag="ACHKAU_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5533985.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2226..>2864
                     /gene="gloB"
                     /locus_tag="ACHKAU_005123"
     CDS             2226..>2864
                     /gene="gloB"
                     /locus_tag="ACHKAU_005123"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO5533986.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQI"
BASE COUNT          764 a          699 c          678 g          723 t
ORIGIN      
        1 tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc
       61 gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt acggattgcc
      121 tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt tgctgcaaaa atcgcccaag
      181 ccgctatttt tagcgccttt tacaggtatt tatgcccgcc agaggcagct tccgcccttc
      241 ttctccacca gatcaagacg ggcttcatga gctgcaagct cttcatctgt cgcaaaaaca
      301 acgcgtaact tacttgcctg acgtacaatg cgctgaattg ttgcttcacc ttgttgctgc
      361 tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat cgccagataa
      421 acttccgcaa ggatctgtgc atcgagtaat gccccgtgta gcgttcgttt actgttatct
      481 atttcgtagc gggcacataa cgcatcgagg ctgttgcgct taccaggaaa cattttcctc
      541 gccaccgcaa ggctatcggt gaccttacag aaggtattgg tcttcggaat atcgcgctta
      601 agcaacgaaa actcgtagtc cataaagccg atatcgaacg ctgcgttatg gatcaccaac
      661 tccgcgccgc ggatatagtc catgaactca tcggctactt cggcaaacgt gggcttatcg
      721 agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac cagccgatcg
      781 ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac ttcaacggca
      841 ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat accggtggtt
      901 tcggtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat ttatgtcaga
      961 cttgtcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct taaacaggta
     1021 gaaattttca ccgatggttc gtgtctgggt aatcctggac ctgggggtta cggcgctatt
     1081 ttacgctatc gcggacgcga gaaaaccttt agcgcaggct acacccgcac caccaacaac
     1141 cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca ttgcgaagtc
     1201 attttgagta ccgacagcca gtatgtccgc caaggtatca cccagtggat ccataactgg
     1261 aaaaaacgtg gctggaaaac agcagacaaa aaaccagtaa aaaatgtcga tctctggcaa
     1321 cgtcttgatg ctgcattggg gcagcatcaa atcaaatggg aatgggttaa aggccatgcc
     1381 ggacacccgg aaaacgaacg ctgtgatgaa ctggcccgtg ctgcggcaat gaatcccaca
     1441 ctggaagata caggctatca agtcgaagtt taagcctgtg gtttacgata ttgccgggtg
     1501 gctccaaccg cctggcgaat tcgtggcttg tttttactct gtttcatcgg atttagcgtt
     1561 aaaggaatag tccgtttccg ggcaacaata agttgtaagc aaccaagcgc aggaatatgt
     1621 gcattcaata gttttccacc gtgtttgttc cacgggagaa cgtggaaacg gctggcgtgc
     1681 agcacttcaa aattcaacaa agagagccag tccagttgcc gcatcagagt aaacatccgg
     1741 ctgttatagg gcgaggtttt tcgcaatacc ggcacaagtt tgcgtaatcc cattaaacta
     1801 atgggattga agccactaat caccagccag ccatcatcaa tcaatacccg atcggtttca
     1861 cgcaataaac gatgcggatc ggtgcaccac ggcaatgtat gtgccagtag acaaacatca
     1921 acggatttat cggcaaaagg aagatgaagt ggatccgcct gcacctggac gggcattcct
     1981 tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat ttctgcgctt
     2041 aaattgccaa tcttaagcag atgaaaacca tacattttag tgaaccacgg gttgagctgg
     2101 cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagcg atcaggagcc
     2161 acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac actaagaggt
     2221 aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg gttttgaatg
     2281 atgaagcagg tcgctgcctg attgtcgatc ccggagacgc agagccagta ttaaacgcca
     2341 ttgccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcat cacgatcacg
     2401 ttggcggcgt aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat ggtccacaag
     2461 agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc ttcgttttgg
     2521 ggcatgaatt tagtgtaatt gccacaccgg gtcacacttt aggacatatc tgttacttca
     2581 gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt aggttgtttg
     2641 aagggacagc atcacaaatg tatcaatcac ttaataagtt aagtgcgtta cctgacgata
     2701 cattggtatg ttgtgctcat gaatatacgt tatcaaatat gaagtttgct ttgagtattc
     2761 ttccgcacga tttgtccata aatgattatt atcgtaaagt taaggagtta cgggcaaaaa
     2821 atcaaataac actacccgta attctgaaaa atgagcggca aatt
//
LOCUS       DAZFJF010000273         2859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFJF010000273 DAZFJF010000000
VERSION     DAZFJF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.275"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..509
                     /gene="agaB"
                     /locus_tag="ACHKAU_005124"
     CDS             33..509
                     /gene="agaB"
                     /locus_tag="ACHKAU_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO5533987.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            548..1351
                     /gene="agaC"
                     /locus_tag="ACHKAU_005125"
     CDS             548..1351
                     /gene="agaC"
                     /locus_tag="ACHKAU_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO5533988.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1341..2132
                     /gene="agaD"
                     /locus_tag="ACHKAU_005126"
     CDS             1341..2132
                     /gene="agaD"
                     /locus_tag="ACHKAU_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO5533989.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2133..>2859
                     /gene="agaI"
                     /locus_tag="ACHKAU_005127"
     CDS             2133..>2859
                     /gene="agaI"
                     /locus_tag="ACHKAU_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO5533990.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLH"
BASE COUNT          693 a          675 c          726 g          765 t
ORIGIN      
        1 atattattca actttatggt gaggattaca caatgagcag tccaaatatt ctcttaaccc
       61 gtattgataa ccgtctggtt cacggtcagg ttggcgtgac ctggacatcc accatcggtg
      121 cgaatctgct ggtagtcgtg gatgatgttg tcgctaacga tgatattcaa cagaaattaa
      181 tgggtattac cgcagaaacc tacggctttg gcattcgttt ctttactatc gaaaaaacca
      241 ttaacgtcat cggcaaagct gcaccgcatc agaagatctt cctgatttgc cgtacgccac
      301 aaacggtacg taaattggta gaaggtggca ttgacctgaa agatatcaac gtcggcaata
      361 tgcatttctc ggaagggaaa aagcaaatca gcagtaaagt ttatgtcgat gaccaggatc
      421 tcgcggactt acgttttatt aaacaacgtg gcgtgaatgt tttcattcag gacgtccctg
      481 gcgatcaaaa agaacaaatc cctgactaaa tctaaaatcg ccttaatatt ggtttgaggt
      541 aataaaaatg catgaaataa ccctacttca gggattatcc ctggcggcgt tagttttcgt
      601 tctggggatt gatttttggc tggaagcctt atttttattc cgcccgataa tcgtttgtac
      661 cctaactggc gctattctcg gtgatattca gactggctta attaccggtg gtctgacaga
      721 gttggctttc gccggattaa cccctgcagg tggtgttcag ccgcccaacc cgattatggc
      781 gggtctgatg accaccgtca ttgcatggtc tacgggcgtt gatgccaaaa cagcaattgg
      841 tcttggcctg ccgtttagtt tgttaatgca gtacgtcatt ctgttcttct attccgcttt
      901 ctcattattt atgaccaaag ccgataaatg cgcgaaagag gcggatacgg cagcgttttc
      961 ccggcttaac tggacaacga tgctcatcgt cgcttcagcg tatgcggtga ttgctttcct
     1021 ctgtacttac ctggcacaag gggcgatgca ggcgctggtg aaagcgatgc ccgcctggtt
     1081 gacccacggc tttgaagtgg ctggcggtat tctgcctgcc gttggttttg gcttgctgct
     1141 gcgcgtaatg ttcaaagcgc aatatatccc ttacctgatc gccggtttcc tgtttgtttg
     1201 ctacatccag gtcagcaacc tgttgccggt tgccgtactg ggcgcaggct ttgcggtgta
     1261 tgagtttttc aatgcgaaat cccggcagca agcgcaaccg cagcccgttg ccagtaaaaa
     1321 tgaagaagag gactacagca atgggatctg aaatcagtaa aaaagatatc acccgtctgg
     1381 gctttcgttc gtcgctgctg caagcgagct ttaactacga aaggatgcag gcgggcggtt
     1441 ttacctgggc gatgttgccg atcctgaaaa agatttataa ggacgacaaa ccgggcttaa
     1501 gcgcggcgat gaaagataac ctcgaattta ttaataccca cccgaatctg gtcggattcc
     1561 tgatggggtt attaatttcg atggaagaaa aaggagaaaa ccgcgacacc attaaaggcc
     1621 tcaaagtggc actgtttggc ccaatcgccg ggattggcga tgcgattttc tggtttactt
     1681 tgttgccgat tatggcggga atttgctcat catttgccag ccagggaaac ctgctggggc
     1741 cgattctatt tttcgccgtt tacctgctta tctttttcct gcgcgtcggc tggacccacg
     1801 tcggttattc agtcggcgtg aaggcgatcg ataaagtgcg agagaactcg cagatgattg
     1861 ctcgctcggc aaccatcctc gggatcacgg taatcggcgg gctgatcgct tcgtatgtgc
     1921 atattaacgt ggtgacatcg tttgccatcg acagtaccca cagcgtcgcg ctgcaacagg
     1981 atttcttcga taaagtcttc ccgaacattt taccgatggc ctacaccctg ctgatgtatt
     2041 acttcctgcg ggtgaaaaaa gcgcatccgg tgctgttaat cggcgtgact tttgtgctct
     2101 ctattgtttg ttccgcattc ggcattttgt aaatggaacg aggcactgct tctggcggtg
     2161 cctctttact taaggaattt catcctgtgc aaacccttca gcaagttgaa aactatacgg
     2221 cgttaagtga acgagccagc gaatatttat tggctgtgat ccgtagcaaa ccggatgccg
     2281 tgatttgcct ggcgaccgga gccacgccat tactgacgta tcattatctg gtagaaaaaa
     2341 tccaccagca gcaggttgat atcagccagc tcaccttcgt gaagctcgac gaatgggtgg
     2401 atctgccatt aacgatgcct ggcacctgcg aaactttcct acaacagcat atcgtgcagc
     2461 tgctggggct gcgtgaagac cagctcatca gctttcgctc cgaagagata aatgagacag
     2521 agtgcgaacg ggtaacgaac ctgattgcgc gcaaaggcgg tctggattta tgcgttctag
     2581 gattggggaa aaacggtcat cttgggctga acgaaccggg agaaagccta caaccggcct
     2641 gccatatcag tcaacttgat gccagaacac agcaacatga gatgttaaaa accacgggtc
     2701 gccccgtgac tcgtgggatc accttaggcc tgaaggatat tctcaatgcc cgcgaggttt
     2761 tgttattggt gactggcgaa ggaaagcagg atgcgacaga acgttttctc acggctaaag
     2821 tctccaccgc tatcccggct tcgtttttat ggctgcaca
//
LOCUS       DAZFJF010000274         2826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFJF010000274 DAZFJF010000000
VERSION     DAZFJF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.276"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(46..>2826)
                     /locus_tag="ACHKAU_005128"
     CDS             complement(46..>2826)
                     /locus_tag="ACHKAU_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5533991.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTELRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPT
                     LRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQH
                     LTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFR
                     QLALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYD
                     PTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYICSVNTVGK
                     SAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVS
                     NEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIE
                     NTSDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGN
                     PPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCRVLGKLSANQIEGDLVKTV
                     GKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDIYCKRRL
                     STVWQILKFLRE"
BASE COUNT          559 a          876 c          731 g          660 t
ORIGIN      
        1 gcggttaatg atttcatgga tagcgtccac catatttggt gtccactatt ctctcaggaa
       61 tttcaggatc tgccagacgg tgctgagacg acgcttacag taaatatcag tatgctctct
      121 ctcatgttta gcgccgctga atgccaccgc cggaataaca atctgccggt caaacggctg
      181 atcgtcataa accctgacgg taatggtccc tgatggccac cgttccggtg cacgggagtc
      241 ccgggggaaa gctttgccca ctgttttaac gagatcgcct tcaatctggt tcgcggacag
      301 ttttcccaga acccgacagt tctcgttaat cgtgacattg ttgagcgtcc cggcgttcgc
      361 attcacactg ccactgatat ccgcattttt agcggtcagc tttccgtccg gtgtcaggga
      421 aaatgccggt ggatttccac cgctggtaat ggtgggggcc gtcaggcgtt tcaggaacac
      481 gtcgttcatg aatatctgat cgccctgacc aacaaacatc ggttttgtgt tgccattcgc
      541 aggattaacc atcgcaatcc tgtccgccgc cagcagcacc tgactctgca tgccgtcaga
      601 ggtgttctca ataccggcac caatacccgc gatataaagg cgtccgtcct gcatctgctg
      661 cagcttcaca gcccacatgc tgttcaggtt attatttgta tcaacctgaa ccttctgtat
      721 ctgctgaatt gccgcactct ggtcttccag tttcttattg acggtctgcg tgatttcatt
      781 gctgacatcc gtaatggacg tcctgatttc agccaggtca ggcgcaagct gaccgttatc
      841 aatctgcgtc cacaactcct gagccagatg ggttttccct atctcgcctt tgaaaaaatc
      901 cagatagccg gatgcatcat cactcggctg gccgacggcc tccacaaatg ccgatttgcc
      961 aacggtgttc acactgcaga tgtaaaaata ataatcatgg cccggcttaa tattgatact
     1021 ggcagctatc cagtacagcg ccgtgccaag atagcgggct gtggtttcaa cctgcctgat
     1081 atccgcaatc cgcttttccg agaaccagaa ttcaaactgt accgtcggat cataaaccgc
     1141 aagatgcggc gtggcggtta tctgaaaata gcccggcgtc agctcaatcc gcgacggcgc
     1201 tgccggtgcg gcaatccgga acgataccga cgccggatcg ccctgctgtc cccacgcatt
     1261 taccgcccgg actgtcagcg tgtaacgccc cagcgccagt tgcctgaagc ggtatgtggt
     1321 ttccgtcgtc cgggccgtgc tgaccagccg ctcactgccg tcatccgctg ccacggtcag
     1381 gcgaagaagg aagctaacgc ccttcaccac cttcggcgtg tcccagcgcg ccagcacctg
     1441 atattccccg ctgtctgcgg tgacttcggc agtcaggtgc tgtaccgctg gcggcgtgac
     1501 accattcacc gtgccgctct ggtcgccgtc aaagtgcgcc ccgttatcca cgatggcttc
     1561 tttttccggt acatgctgca cggcagtgat ggcatacgtg ccgtcatcgt tctcacggat
     1621 actcacgcag cggaacaggc gctggcgcag cgtcggcaac ttcagccccc atacgctgta
     1681 ttcagcaacg ccgtcaggaa cacggctcac tttcaccttc acgccgtcgg tgacggactg
     1741 aacctccacg ctgaccggat tgccacttcc gtcaaccagg cttatcagcg tggtaccgga
     1801 ggatggcagc gtgatttcac ggtcgagcgt cagcgtccgg gtctggctgt tcaccgccag
     1861 cacgcgcccg ccggtgctga taccggcata gtcatcatcg cagatttcaa tgacatcgcc
     1921 cggcacatgg cgaagccctt ctgcgcccac gctgaagtcc acggtctgcg tttccagcag
     1981 ttctgtttta atcagccaca gcccggcgcg gtgtgcctgc ccccggctgg tacagccaaa
     2041 agcatccatc ttcgtgacgt tacgaccgta acgggcaatg gcctgcgtat cctccacaag
     2101 ctctgtcgcc gtctcccagc cgttgttcgg gtcaatccag ttcacctcaa cggcattatg
     2161 gcggtccttc agggcgctga agctgtagcg gaacggcgcg ccatcatccg gcatcaccac
     2221 attactgcgg ttataggtcc acaccttatc cgacggtcgg tcctgcacga acgtcagcgt
     2281 ctgcccgttc cataccggca tacagcgcat cgccgagcag aaatcactga gcacatccca
     2341 cgccttgcgc tgtgtggtca ggtacgcatt acaggtgatg cgcagctccg tgccgccaaa
     2401 gccgtccggc actgactggt cgcagtactg gccgatgaca tacagcgccc atttgtccac
     2461 atccgccgca ccaagacgtt tccccatgcc gtagcgcggg tgggtcagca tatcccacag
     2521 acaccaggcc atgttgttgc tgtatgctgg ttttaacgtt ccgtcccaga taccgctgta
     2581 ttgtcgcgtc tgcgggttat agttcgacgg cacctgcaga atgcgcccgc gaagatgata
     2641 attacgactc acctgctggc tgccgaactg ctccgagtcc acctgtacgc cgaccagtgc
     2701 cgtgttcggg tagcactgtt tcacatcgat gatttcggtg tatgacgacc agagcgtttt
     2761 gttctgcagc tggtctgtgg tgctgtccgg cgtcatcctg cgcatccgga tattaaacgg
     2821 gcgcgg
//
LOCUS       DAZFJF010000275         2797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFJF010000275 DAZFJF010000000
VERSION     DAZFJF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.277"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..251
                     /locus_tag="ACHKAU_005129"
     CDS             <1..251
                     /locus_tag="ACHKAU_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO5533992.1"
                     /translation="EMLQDKNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDII
                     EAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            279..1237
                     /locus_tag="ACHKAU_005130"
                     /pseudo
     CDS             279..1237
                     /locus_tag="ACHKAU_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1249..1710
                     /gene="yibG"
                     /locus_tag="ACHKAU_005131"
     CDS             1249..1710
                     /gene="yibG"
                     /locus_tag="ACHKAU_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO5533993.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2345..>2797
                     /locus_tag="ACHKAU_005132"
     CDS             2345..>2797
                     /locus_tag="ACHKAU_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO5533994.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDDKNIKL"
BASE COUNT          907 a          490 c          626 g          774 t
ORIGIN      
        1 tggagatgct tcaggataaa aatgaagaag tccgtattga agcaataatc ggactttcct
       61 acagaaaaga taaaagggtt ttatctgttt tatgcgatga gttaaaaaaa aatactgttt
      121 atgatgatat cattgaagct gcgggtgaat taggtgataa aacgctactt cctgttttag
      181 atactatgtt gtacaagttt gatgacaatg aaattataac ttccgctatt gataagctga
      241 agcgttcata gcgtgagttg cctatgcaca gtgggggatt cccgccggca cggtgcagat
      301 gctcgaccgg ctggaaagtg aaatcctggc tgaccgggtg agtgaggaaa gccgccgctg
      361 gctggcatcg tgcggcctga ccgtggagca gatgaaaaac cagatggacc cggtgtacac
      421 gccggcgcga aaaatccacc tgtaccactg cgaccatcgc ggcctgccgc tggcgctcat
      481 cagcacggaa ggggcaacag cgtggtgcgc agaatatgat gaatggggca acctgctgag
      541 tgatgagaac ccgcatcatc tgcagcagct cattcgcctg ccgggtcagc agtatgatga
      601 ggagtccggc ctgtattaca accgccaccg ctattatgac ccgctgcagg ggcggtatat
      661 cactcaggat ccgattgggc tgaagggggg atggaatttt tatcagtatc cgttgaatcc
      721 ggtcataaat gtagatccgc aaggtttggt tgatataaat ttataccccg aaagtgatct
      781 tatccattct gtagctgatg agattaatat cccaggcgtt ttcacaatcg gggggcatgg
      841 tacccccaca tctattgaat ccgcaacgcg cagtatcatg acagctaaag atctagcata
      901 tctaattaaa tttgatggga attataaaga tgggatgaca gtttggttat tttcttgtaa
      961 tacaggtaaa ggacaaaatt catttgctag ccaattagct aaagagttac atacaaatgt
     1021 aataggacct gacacgctat ggacgtggtg ggggcgagga actaatggta agttaaaaat
     1081 ggatacagtg ctaacagcac caacgaacct taattcaaat aaggatctaa tggctataac
     1141 aacaaaagac cttggtaatt ggataacata tgggccatct gggcacccca tttctaatat
     1201 gcaaggtacg ccagaaaaac ccagtgatat aagataggtt gtagatgtat gaaagcatgc
     1261 ttgttactat ttttttattt ttctcttatt ggtcaattgc atggtgctga tgtgaaaata
     1321 aaagaaaacg aaagtgtgat gggatctaca gcaatgacct atgatttaag tgaagaaaag
     1381 ctgatgaaac tgaagtataa atcacaacat ggtgattcag aggcatcatt tcgactatat
     1441 caatattact gctttactaa aaataatatt gataaacaat tgcgattctt ggaaagatca
     1501 gcatctcagg ggaatgttac agcgcagttt aattatgggg tctttttatc agatacaaat
     1561 ccaacattat cagaatatta taatttgaat agagctattt attggatgga attcgctgta
     1621 aataacggta atattgatgc aaagagcaaa cttcaagagc tcaaaaagct aaagcgaatg
     1681 gatagaagga agaataagga gaatccgtaa aagcaatagt tcatcagact gccgcagcag
     1741 tagtatgatg atgaatcagg gctgtactat aactattatc ggtaacatta accgcagcag
     1801 gggcggtata acactcaggc tccgattggg ctggaaggta ggtggaattt ttatcagtat
     1861 ccgttgaacc cgatttctgg aatcgatccc cttgggatta gccacctgtt tatacagtat
     1921 aactttaggt atgctttcgt gtgtttcaga tataccatgt gatgataatt tttatgatgt
     1981 gcttagtatt ccagtcgctt ctggtaataa tatgcaatgt aaaaataatc cccattgtac
     2041 acatttataa atagatggcc aatacctcaa ggcctatgga gttagaatgt taatgacccg
     2101 ggagcaagca atagaaaacc tgacggcata cgattagtac cgtcagttaa tacaggaact
     2161 tataatcgca atggattttc aatacatagt tgtttaaacg cttttggtcc gtctttagga
     2221 cctcgattct gttccgaggg ttgtataact ggtttatcta atgacatgca aaaattaaat
     2281 gagttaatat tttcagagcc agatagcaca ttaacagtta cggactaaaa aatatggcaa
     2341 ggttattaac aattgtattt ttttctctat ttctgcgagt gcagttactg caagacaatt
     2401 tcagagagag ttagatttgg cagacaaagc aatattatgg gcactaatat cagccagtac
     2461 taaagaggga cgaaaagcgt gttcactaag ctattttgcc tgtaaggccg cagaggctga
     2521 actcggactg gcatatatgg ctgcgaatga taacaaggaa tttctcacat ccttatctaa
     2581 tataatgagg tacaaaatag atgctggact ttccgaatcc tatacatgct atttattaag
     2641 taagggaaag atcataagac catatctgaa aaatctaaat ccactccaac ttgccgctga
     2701 ttgcattgaa acagtaaata aaataaaaga taagaataaa aaaatcattg atataaattc
     2761 agttaatatt tgtagtgatg ataaaaatat aaaattg
//
LOCUS       DAZFJF010000276         2773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFJF010000276 DAZFJF010000000
VERSION     DAZFJF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.278"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..2388
                     /gene="ydeP"
                     /locus_tag="ACHKAU_005133"
     CDS             109..2388
                     /gene="ydeP"
                     /locus_tag="ACHKAU_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO5533995.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKWMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2636..>2773
                     /gene="safA"
                     /locus_tag="ACHKAU_005134"
     CDS             2636..>2773
                     /gene="safA"
                     /locus_tag="ACHKAU_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO5533996.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     IS"
BASE COUNT          730 a          654 c          674 g          715 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     1501 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     1561 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     1621 tatatcctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     1681 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     1741 ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa tcgcccaggc aacactaccc
     1801 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     1861 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     1921 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     1981 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2041 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2101 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2161 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2221 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     2281 acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc attaagtggc
     2341 attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca
     2401 tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat cgcgcaattt
     2461 gctcacattg attaaattaa accacacact ataagatata aacctctgcc tacagctgta
     2521 agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca gcctgttata
     2581 ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca
     2641 tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt
     2701 aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg caattgatca
     2761 gctgggtatt tca
//
LOCUS       DAZFJF010000277         2737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFJF010000277 DAZFJF010000000
VERSION     DAZFJF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.279"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHKAU_005135"
     CDS             complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHKAU_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO5533997.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHKAU_005136"
     CDS             complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHKAU_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5533998.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1944..>2737)
                     /locus_tag="ACHKAU_005137"
     CDS             complement(1944..>2737)
                     /locus_tag="ACHKAU_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5533999.1"
                     /translation="FRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIP
                     SPQLGDGFIAFILAVVMAIILSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          755 a          733 c          649 g          600 t
ORIGIN      
        1 catcaaatca agcaaaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat
       61 tcaattaatg gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgtaa
      121 aaaattcatc aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt
      181 cagcgacggt tcgcgcaaac cccatctcat gcgttacaca caacattgtc atacccgatt
      241 gcgccagccc aatcatcgta tccagcacct ctttcaccat ctccggatcg agcgccgacg
      301 ttggctcatc aaacaacata attttcggct tcatacacag cgaacgcgca atggcaacgc
      361 gttgctgctg accaccagaa atttgtccgg gaaacttatg cgcatgttcg gcaattctca
      421 cccgctcaag gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc
      481 aaatcggtgc cagggtacag ttctgtagaa cggtcagatg agggaagaga ttgaaatgct
      541 gaaagaccat tcccacttcc tgccggacgc gctcaatatt gcggatatct tcattaagtt
      601 cgatgccatc taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa
      661 tggttgtcga tttaccggaa cctgaagggc cacacagaac gatccgttct cccggttgca
      721 cggttaaatt tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca
      781 gcgtaatcat cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt
      841 gtacgcccgg tgttaaaacg tttttccaga tgctggctat agcgcgacat gctgaaacag
      901 aagatccagt agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggtg
      961 ggatcaacgg ttgcctgctg aacgctactg aaaagatcga acaacccgat aatgatcacc
     1021 aggctggtat ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc
     1081 aacgcctgtg gcagaataac cagcccctga gttttccagt aacccaacgc cagcgactct
     1141 gccgcttcat actgcccttt aggcagtgcc tgtaatccgc ctcgcacgac ttccgcaaca
     1201 tatgctgact gaaacaggat cacgccaacc agcgcgcgga tcagtttgtc gatactggtg
     1261 ccttctgcca taaacaacgg cagcatgacc gaagacataa acaatacggt aatcagcggt
     1321 acgccgcgcc agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag
     1381 cggcgaccta acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aacggatgca
     1441 ataattaaag ttagcgtcag cccgccccat tgccgggttt caacccgctc cagaccaaaa
     1501 aaaccgccat acatcagcca ccagacaatc agtgggtaaa tcaccgccca gaccgcaata
     1561 tagcgaccgc gatgcgggag tattttccag aacattggtg cgattgacac aaggccaatc
     1621 agtaatgcca ggttaattcg ccagcgttgg tcgtgtgggt aaagtccata cataaactga
     1681 ccaaatcgtt cgtggatgaa gacccaacag gcaccggctt ttgtgcagtc ggcacgcgtt
     1741 gagccaaccc aattagcctg caaaaatgcc cagttcagca acggtgggat cagttcccac
     1801 atcaaccata tgcagccaat agtcagcagg ctattgctcc agctggagaa cagatttttt
     1861 cgcacccaca ccatcgcgcg gcttgagtta tggctcgctg ggcgcgaggg ctgagacagc
     1921 aatacttttg tcatcatgac tccttagcgt tcaacgatcg cgatgcggcg gttatagata
     1981 ttcatcagca gcgagatagt caggctgata atcagataga ccgacatggt catggctatc
     2041 gtctcgatgg cttgccccgt ctggttcagc acggtgccag caaacagcga aaccatatcg
     2101 ggatagccaa tagcggcggc aagagaggag tttttgacga tgttgagata ctggctggtt
     2161 aacggcggaa taatcactcg cagtgcctgg ggaataatga cctggcgtag cgtaaccggg
     2221 ttgggtaatc ccagtgaccg agccgcttca tgttgaccat aaggcactgc ctggatccca
     2281 gcgcggataa tctcggcgat aaatgcagat gtataaaccg aaagtgccag cgttaaggct
     2341 gccagttcag gaattaaaac catcccgccg cggaagttaa agcctcgtag ggctgggaca
     2401 tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta aaggcaaacc aatgatcaac
     2461 actgcggcga tcggccaggt gcggcgcagt tgtccggtct ttatctggta tgttttatta
     2521 aagcggaata gcccaacaga aaggattata gccataacaa cagccaggat aaacgcaata
     2581 aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa aaaggccaga
     2641 tcaaacgcgc tcacagcctg gcgtggtccg ggcaaattgc gcaacacggc aaagtaccag
     2701 aagaagattt gcagcagcgg gggaatatta cggaaga
//
LOCUS       DAZFJF010000278         2669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFJF010000278 DAZFJF010000000
VERSION     DAZFJF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.280"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHKAU_005138"
     CDS             complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHKAU_005138"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO5534000.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHKAU_005139"
     CDS             complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHKAU_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO5534001.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          664 a          658 c          646 g          701 t
ORIGIN      
        1 aaggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc tcagcccgct
       61 tttcagcggg cttcattgtt tttaatgctt acagcactgc cacaatcgct tcgcacagca
      121 gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca accgcatata
      181 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg aaggagaaca
      241 tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat
      301 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc tcttgttccc
      361 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
      421 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
      481 tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
      541 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
      601 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
      661 accagccttt ctcaactgag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
      721 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcgt
      781 tcaggctgtt aatcagtgca tcgaagtcaa gggtgtggtt ttctgcatca taataagcgt
      841 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
      901 tgctcaccca cactcgctta acgctggtat tttttgccag gaaatcggca gctacgcgta
      961 gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
     1021 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
     1081 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
     1141 tcaataccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
     1201 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
     1261 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
     1321 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
     1381 caagacgtga gattgctctg gaaggcataa aaaaaacagg accaaagtcc tgttttttcg
     1441 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
     1501 tcagaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
     1561 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
     1621 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat ggacggacgc
     1681 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
     1741 ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc accgtagttc
     1801 gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg ttcagctttt
     1861 ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc accataagca
     1921 ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc gtcgccgtta
     1981 gaacggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa gttcaggcca
     2041 tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac acgaccaacg
     2101 aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc ggtgtaaccc
     2161 agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaac gtcagcgtat
     2221 ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc gccttcagag
     2281 ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga attgatttga
     2341 gtttcccctt taaaaccaag acgggcatag gtcatgtcgc cattgccacc gtaactgttt
     2401 tcaccgttac ccttggaaaa ataatgcaga ccaacagctt taccgtacag atctactttg
     2461 ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa cagagcaggg
     2521 acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg ttttttttat
     2581 gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac tattgatgag
     2641 agtttggtgt ctttatgtgt ctgcaggca
//
LOCUS       DAZFJF010000279         2669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFJF010000279 DAZFJF010000000
VERSION     DAZFJF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.281"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..266)
                     /locus_tag="ACHKAU_005140"
     CDS             complement(<1..266)
                     /locus_tag="ACHKAU_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534002.1"
                     /translation="MIQYLVKNQVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAGCSEDGLKFWLKQLSNFGVIEIKELSFSTFNLKRLDKEIEFI"
     gene            complement(278..970)
                     /locus_tag="ACHKAU_005141"
     CDS             complement(278..970)
                     /locus_tag="ACHKAU_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5534003.1"
                     /translation="MINQLTFTKHYDTFDNVSKIYSDKFPQGKDLDLLHIVLYFRFLS
                     YQENNLNCYESHETLAKIFKSSASTIKRKINDLKEMGLLETSPHPDPYISSLIYNALP
                     LTDAHITPPGESSLSDLSEAEEAQEQPKGHKQPSFDVLDDDWDTPLPWETEETPVSSS
                     EKVANDNEEDVLKNFASLIIKQKNTRTGGASFIEFATSLAYKHRLKLPNGIEAYFAKK
                     HPKVYEDFDIPF"
     gene            complement(1024..1194)
                     /locus_tag="ACHKAU_005142"
     CDS             complement(1024..1194)
                     /locus_tag="ACHKAU_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534004.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSRRITVTDYILDML
                     KREAETLSNKSK"
     gene            complement(1214..1402)
                     /locus_tag="ACHKAU_005143"
     CDS             complement(1214..1402)
                     /locus_tag="ACHKAU_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534005.1"
                     /translation="MKWFTPEHVVQAFKKGELTRHQVVMNRNMARSRGYPERAACFNE
                     ALKIIDELRKNEKESETE"
BASE COUNT          794 a          488 c          476 g          911 t
ORIGIN      
        1 tagatgaatt ctatttcttt atctagtctt ttgagattga atgtagagaa agataattct
       61 ttaatttcta taacaccgaa gtttgatagc tgtttcagcc agaattttaa accatcttca
      121 gagcaccctg ctcgatgtaa cacatcgcta tattttagcg gtgttggctt tcctttatag
      181 aggtaagcaa gtgtttcgta gatgcgtttt cctgtgtcgt tacactgaat tctgtctact
      241 tgatttttta ctaaatattg aatcataacc ctccttatta aaatggaata tcaaagtctt
      301 cgtatacttt gggatgtttc ttagcaaaat atgcctcaat accatttggt agctttaacc
      361 tgtgcttata ggctaacgat gttgcaaatt ctataaatga tgctcctcct gttctagtat
      421 tcttctgctt gataataaga gaggcaaaat tctttaacac atcctcctca ttatcattag
      481 ctaccttttc acttgatgaa acaggtgttt cttccgtctc ccacggtaaa ggcgtatccc
      541 aatcatcatc aagaacatca aaagaaggct gtttatggcc tttaggttgt tcctgtgctt
      601 cttctgcctc agaaagatca gagagtgatg attctcctgg gggagtaata tgtgcatctg
      661 tgagagggag agcattgtaa atcaaagatg aaatatacgg atcaggatgt ggggatgttt
      721 ctaataatcc catctcttta agatcgttta tcttacgttt aattgttgac gctgaagact
      781 taaaaatctt agctaatgtt tcgtgacttt cataacaatt aaggttgttt tcctgataac
      841 taaggaatcg aaaataaagc acaatatgta atagatctaa atcttttcct tgagggaatt
      901 tatcagaata aatctttgat acattatcaa acgtgtcata gtgttttgta aaagttagtt
      961 ggttaatcat taaaaatctc cttgatgtaa tcacgctcta tggcgctact atggtgataa
     1021 aaatcacttg cttttatttg agagtgtttc agcctctctt ttcaacatat caagaatgta
     1081 atctgtgaca gtgatccgtc tacttctggc ttctttaaat aacacctgaa atacttcatc
     1141 aggaatatga aagcaagtgc gtggttgtct tttcttttct gtagtttcgc tcatagtttt
     1201 ctccttctct tctttactct gtttcagatt ctttttcatt ttttcttagt tcatcaataa
     1261 tcttgagtgc ttcattgaag caagctgctc gctctggata acctcgactc cttgccatat
     1321 tacgattcat tactacttgg tggcgtgtaa gctcaccttt tttaaatgcc tggacgacgt
     1381 gctcaggtgt aaaccatttc ataaaaaact ccttattcag ttaaaagaaa aaacaccttg
     1441 caagcctgac ggcttcaaag tgcattgatt ttgattattt agagaagaat gtttttctgt
     1501 ccctctaata cctattatta catataaaga aacatttgtc aatacctttt cataaaaaat
     1561 ctgatctttt tcatgcgctt acattttttc ttagcaattc gtgctctcct atgtagtgct
     1621 attcttcctt attgtctaaa acgctctaaa acgcgttcta acgagatttt gataaaagca
     1681 atacattccc ttatctaatg gctgtttagc tcgccagagg cttatttaag tggcttattt
     1741 tgtatttgtt agatgatgat agatataaaa cagagagtca ctttataaaa cgctctgtaa
     1801 agccctgtag tgagtttatt atatgtaggc aatgcgtttc cattccctac cctgcttaat
     1861 gttgttagag aagcatttag accccttatt tttaattacc taaagtatcc tctatacatc
     1921 tctgttatga aacacctctg aatccttcat aaaacacatt atgacgcaaa gaacactttt
     1981 ctaaggtgat tcccttcctg aatgctgttt cttgcgtcag agagctacac agagcttata
     2041 aagatattat taataataaa gtagataaag taagtaagat aagtaagtaa gataactaag
     2101 atagataaga ttaataagat cagaattctc tttcggtgtc gcctctggct tttccaccga
     2161 aaacaattac acgactacag aatgtaggaa gtgaaattgt attgaggctt agtgaaatta
     2221 cacgaggaaa gcagagtgta attgagcttt agccgctctc ttcgagtaat tacacaacta
     2281 cagagaataa cgagagttat taaatgtagg aagtggaatt aagaggtaga gagttacaca
     2341 taagcaatca gtatgattgg ttaggtgatt actcttctgc gcacagcgca atagtatttc
     2401 ttcgtgggcg ttaccgccac gatagttaca cgaagaaagt aacgctttgc tttcggtgtg
     2461 agcggaacgg gccagccacg aagcccgaaa ggtagcggag taaggctgct tgccagagag
     2521 tgaagctaac gccggaaggc aaaagatcat atatatatag tataaagaat attattatga
     2581 tctaattaat aatctaatat agttttatta atagtcttat tgtagttcac agtgacctac
     2641 cccctagctc actatgacct accccctag
//
LOCUS       DAZFJF010000280         2666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFJF010000280 DAZFJF010000000
VERSION     DAZFJF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.282"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1836
                     /gene="rhsB"
                     /locus_tag="ACHKAU_005144"
     CDS             <1..1836
                     /gene="rhsB"
                     /locus_tag="ACHKAU_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO5534006.1"
                     /translation="WLSGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQ
                     SQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTSVHTTAANLDIRIPYA
                     TDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTD
                     DERTHRYHYDSQHRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSL
                     SRKPEVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLAD
                     TLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMD
                     PVYTPARKIHLYHCDHRGLPLALISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLP
                     GQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMW
                     EDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDN
                     SGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIV
                     KSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            1808..2191
                     /gene="yhhH"
                     /locus_tag="ACHKAU_005145"
     CDS             1808..2191
                     /gene="yhhH"
                     /locus_tag="ACHKAU_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO5534007.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            2296..2666
                     /locus_tag="ACHKAU_005146"
                     /pseudo
     CDS             2296..2666
                     /locus_tag="ACHKAU_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315903.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
BASE COUNT          803 a          595 c          691 g          577 t
ORIGIN      
        1 tggctgtcag gcatgaaact cggcgacaca ccgctggtgg agtacacccg cgaccgcctg
       61 caccgggaaa cgctgcgcag cttcggtcgt tatgaactca ccaccgctta cacccctgcc
      121 gggcagttac agagccagca cctgaacagc ctgcagtatg accgcgatta cacctggaac
      181 gacaacggcg aactcatccg catcagcagc ccgcgccaga cccggagtta cagctacagc
      241 accaccggca ggctgaccag cgttcacacc accgcagcga atctggatat ccgcatcccg
      301 tatgccacag acccggcagg taaccgcctg cccgacccgg agctgcaccc ggacagcacc
      361 ctcagcatgt ggccggataa ccgtatcgcc cgtgacgcgc actatcttta ccggtatgac
      421 cgtcacggca ggctgacaga gaaaaccgac ctcatcccgg aaggggttat ccgcacggat
      481 gatgagcgga ctcaccggta ccattacgac agtcagcacc ggctggtgca ctacacgcgg
      541 acacaatatg aagagccgct ggtcgaaagt cgctatcttt acgacccgct gggccgcagg
      601 gtggcaaaac gggtatggcg acgtgaacgg gacctgacgg gctggatgtc gctgtcacgg
      661 aaaccggaag tgacctggta cggctgggac ggcgaccggc tgaccacgat acagaacgac
      721 agaacccgca tccagacgat ttatcagccg gggagcttca cgccactcat cagagttgaa
      781 accgccaccg gtgagctggc gaaaacgcag cgccgcagcc tggcggatac ccttcagcag
      841 tccggcggcg aagacggtgg cagtgtggtg ttcccgccgg tgctggtgca gatgctcgac
      901 cggctggaaa gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg ctggctggca
      961 tcgtgcggcc tgactgtgga gcagatgcaa aaccagatgg acccggtgta cacgccggcg
     1021 cgaaaaatcc acctgtacca ctgcgaccat cgcggcctgc cgctggccct tatcagtaag
     1081 gaaggggcaa cagaatggtg cgcagaatac gatgagtggg gcaacctgct gaatgaagag
     1141 aacccgcatc agctgcagca gcttatccgc ctgccggggc agcagtatga tgaggagtcc
     1201 ggcctgtatt acaaccgcca ccgctattat gacccgctgc aggggcgata tatcactcag
     1261 gatccgattg gactgaaggg gggatggaac ctgtatggat atcaattgaa tccgatatca
     1321 gacatcgacc ccctgggttt atctatgtgg gaggatgcaa aatcgggggc atgtactaat
     1381 ggtctttgcg gcacactatc cgctatgata ggtccagata aatttgattc tatagatagc
     1441 accgcatatg acgccttaaa taaaataaat agccaatcta tttgcgaaga taaagagttc
     1501 gctggtttaa tatgtaagga taatagtggc agatatttct caacagcacc taaccgagga
     1561 gaaagaaaag gatcatatcc attcaatagc ccttgcccta atggtactga gaaagtatca
     1621 gcttatcata ctcatggtgc agatagtcat ggagaatatt gggacgaaat attttcaggt
     1681 aaagatgaga aaatagttaa aagtaaagat aacaatatca agtcatttta tttaggtacg
     1741 cccagtggta attttaaagc aatagataac cacgggaagg aaataacaaa cagaaaagga
     1801 ttacctaatg tctgcagagt tcatggtaat atgtaaaaaa atattgttta ggaactgtgt
     1861 cattgtatct ttgtttgttt ttacatacaa cacatgggcg cagtgtaata ataatattaa
     1921 aataatgcgc aagtatgaaa gtgaaggtaa atataccgtt agaaatttgg ttaaaaataa
     1981 agctatagca ttggaattag ctgagatata tgttaagaat cgttatggac aggatgccgc
     2041 agaagaagaa aaaccatacg aaatcactga gttaacaaca agttgggttg ttgaaggtac
     2101 cattcactca gaccaaattg ctggtggggt ttttattata gaaataggca aaaatgatgg
     2161 gagaattctg aattttggcc acggaaaata agtatgtaag gataatcaaa catcgtgtgc
     2221 gctgatggca gagtaggtgg aggactccag acagtcaaac gatagaaaaa gatagccttt
     2281 atggaggttc ctgcaatgtc aaatacatac cagaaaagaa aggcaagtaa agagtatggt
     2341 ttatataata aatgtaagaa actaaatgat gatgaattat ttcgcttact tgatgatcgc
     2401 aattccttga aaaggatttc atctgccaga gtattacagt taagaggggg gcaagacgct
     2461 gttagattgg caattgaatt ctgcactgac aaaaattata tccgtagaga tatcggagca
     2521 tttatactcg ggcaaatata aatttgcaaa aaatgcgaag ataatgtttt taatattttg
     2581 aacaatatgg cattgaatga taagagtgct tgcgttcgag ctacggcaat cgagtcaacg
     2641 gccagggaaa gatcatcaaa agttca
//
LOCUS       DAZFJF010000281         2662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFJF010000281 DAZFJF010000000
VERSION     DAZFJF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.283"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..488)
                     /locus_tag="ACHKAU_005147"
     CDS             complement(<1..488)
                     /locus_tag="ACHKAU_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFO5534008.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVEVVFEHDVNTKAT
                     NK"
     gene            complement(501..923)
                     /gene="ydaT"
                     /locus_tag="ACHKAU_005148"
     CDS             complement(501..923)
                     /gene="ydaT"
                     /locus_tag="ACHKAU_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5534009.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKTQREKIQKLIPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(920..1174)
                     /locus_tag="ACHKAU_005149"
     CDS             complement(920..1174)
                     /locus_tag="ACHKAU_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5534010.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADVILTIGRNKTTTKKLMGVDS"
     gene            1254..1673
                     /locus_tag="ACHKAU_005150"
     CDS             1254..1673
                     /locus_tag="ACHKAU_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5534011.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            1972..2124
                     /locus_tag="ACHKAU_005151"
     CDS             1972..2124
                     /locus_tag="ACHKAU_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5534012.1"
                     /translation="MDTIDLGNNASLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LAKNTS"
     gene            2545..>2662
                     /gene="kilR"
                     /locus_tag="ACHKAU_005152"
     CDS             2545..>2662
                     /gene="kilR"
                     /locus_tag="ACHKAU_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO5534013.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKR"
BASE COUNT          734 a          614 c          581 g          733 t
ORIGIN      
        1 tctttattag ttgcctttgt gttgacatca tgttcaaaca ccacttcaac atctgtttga
       61 acacctgtta aatttctctc ttgttttgtt tgaacatctg cttcctttct gcttcttctg
      121 gcctgaacag atgcttttcc tgcggctgat tttttggtta atttttccct gactgatgcc
      181 agatcttcct caatccgaag atgcacccat tcctcgccgt tatcgcaaaa aaactcctgc
      241 aaggatggtt caacatcagc ccatcgctcg ttagtcagac gggcaatttt tgccagcctg
      301 tttttaggta ttggctttcc tgtttgccag taattgaaca tcagcaacaa atacgcacca
      361 tgctcctctg ctgacaaatg catggtgtca gccaggtaat cagctatgta cagttgcatg
      421 tatggtaatg cggccataat tgccccgtat gatgctgccc ggtggcttag aataagcaca
      481 aacagcatgg aaacttttgc ttaatgaaca atgacagaat cgtcggaaga cccgccgccg
      541 ctgaaatgcg ctttccggta aacggcttgg actgcatcat catgcgcatc aattgccgta
      601 cttaacgctt cctgcgccgc tagtaatgca cggcgttcca gggtatcgaa gatgcagagt
      661 cggtgacgca gctcgcgcgg aaggattgcc agaattgctg ggatcagctt ctgaattttt
      721 tctctttgcg ttttcgtttc acctttcaac caacggtgat agatattctg ctgattattc
      781 cagtccttgc ctggtaccag gggcaattcg ccgcccccct ggcgcagata ttcttcagta
      841 atggcattgg ctacccatgc ctgccctttt tcggctgcca gggctaacaa cactgactcg
      901 atgtgctcat gcttgatttt catgaatcaa ctcccatcag ctttttcgta gtagttttat
      961 ttctgccaat agttaaaatt acatcagcag aaaataatcc gtttgatgca tgagcgattt
     1021 tttcagcgta atttgtttcg ccggtatatt ctgtgcgagg caattttccg ttatccatcc
     1081 atttgtagat tgctctttgg ctgacaccac aaacgtcggc cacaacagaa acgcgaacag
     1141 ttttgattac atcttcaagt gttttctggt tcatatcacc ctcacaatgt gaactttgag
     1201 tacatgctat aacagaactg acagtacatt caagagcgaa tatcattgaa cttatggttc
     1261 atgaagataa agcgcgtaaa gagttcgcca gtaggcttgc gctagcctgt gaaaacgctg
     1321 gttatgaaca acatggaagg caggcagaaa ttgcccgtcg aatgaaatta acaccaaaag
     1381 cggttagcaa atggtttaat ggcgaaacaa ttcctcgccg agagaaatta agggaattag
     1441 caacactcat tggaacaaca ccaacctatc ttttgggaga ggatacagaa gaaagtggac
     1501 aggtacgttt ctatcaggag ttaaatccaa gacaaaaaat catcattgac cttctggacg
     1561 agctccctga cagtgagaca gatgaacttt taaaaactct tgaggagaaa aaacagaagt
     1621 acaatgcaat ttacgaagag ctagcacgaa agaaaaaaca aaaagcctct taaaccagca
     1681 taaatccggt agcgtccccc tccgggtttg tgcttcactt tatcccatct cattttttta
     1741 cacacaaaat gtacttaaag cactttacat caatgaacac aaagtacatt atatacctac
     1801 caacccaccc cgccccacag aacgcagggc aatacttcga gttacccggc agtggtcagg
     1861 ggttaagtag ccagcccgag gcgtatgaac atgacggcag ggttcaactt taataactat
     1921 gcagcaggtt tttgttccgc taccccggcg ttaaggggaa atgaggtcag catggatact
     1981 atcgatcttg gcaacaacgc atctctggtg tacggcgtgt ttccaaacca ggacggcacg
     2041 ttcaccgcaa tgacgtatac caaaagcaaa acgtttaaaa ccgaaaatgg tgcccgtcgc
     2101 tggcttgcca aaaataccag ctaaaccatt tattggatta attcaatatt cttgctgtag
     2161 gggtatagcc gaggccacca aagcccggag gtggtgaaat aaaaccgggc acaacacgaa
     2221 ggcgcatttc cggtatccat aaagagtcgg tcttgtctgt taaatttaaa tggtgggagt
     2281 gcgcctccgg ttgtgaataa caacactgct gtgtgtagtc ttggcggcat cagttttttc
     2341 ttgaagttcg actgatgtcc gcccttttta aagtgaattt tgtgatgcgg tgaatgcggc
     2401 taagcgcacg cggcacagtt aaaagtcatg ttagtcctta ttggtttggg tgggaaagcc
     2461 gactgtaatt gttaactggt tgcagtcacc tggaggcacc aggcaccgca tcaacaaagt
     2521 tcatttgtaa aaatggagat aattatgatt gcacatcact tcggaactga tgaaatacca
     2581 cgtcagtgtg tgactcctgg cgattatgtt cttcatgaag gccggacata tattgcctcg
     2641 gcaaacaata ttaaaaagcg aa
//
LOCUS       DAZFJF010000282         2657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFJF010000282 DAZFJF010000000
VERSION     DAZFJF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.284"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..710
                     /gene="tnpA"
                     /locus_tag="ACHKAU_005153"
     CDS             33..710
                     /gene="tnpA"
                     /locus_tag="ACHKAU_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO5534014.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKAU_005154"
     CDS             710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKAU_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5534015.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1077..2648
                     /locus_tag="ACHKAU_005155"
     CDS             1077..2648
                     /locus_tag="ACHKAU_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO5534016.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          639 a          728 c          739 g          551 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggctggttgc aggtatcacc gatatgcgaa atggctttaa
      781 cggcctggca tcaaaagttc agaacgtcct gaaggatgac ccgttctccg gacacctgtt
      841 catcttccgc ggacgccggg gtgaccagat aaaagtgttg tgggctgaca gtgacggact
      901 gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc tggccagtca cccgtgacgg
      961 caaggtgcac cttactccgg ctcagttatc catgcttctt gaaggtatca actggaagca
     1021 cccgaaacga acggaacgcg ctggaatccg catataaccc gttgtaaagt gaggatatgg
     1081 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     1141 ccatccgcca gatggctgaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt
     1201 ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca acgtatgcag ttcggtaaaa
     1261 gctcagaaaa acttcgtgca aaaaccgaac ggcagataca ggaagcacag gagcgaatca
     1321 gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca atatgacccg gtactgccat
     1381 ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc ctcacttccc cgtgaaaccc
     1441 gggttatccg gccggaagag gaatgctgtc ctgcctgtgg tggtgaactc agttctctgg
     1501 gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac
     1561 aacgtccgaa acaggcctgt tgccggtgcg accatatcgt gcaggcacca gtaccttcaa
     1621 aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtc accgggaaat
     1681 atgcagacca tctgccgtta taccgccagt cagaaatata ccgtcgtcag ggagtggagc
     1741 tgagccgtgc cacactgggg cgctggacag gtgctgttgc tgaactgctg gagccgctgt
     1801 atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt ccatgctgat gatatccccg
     1861 tcccggtcca ggagccgggc agcggtaaaa cccggacagc ccggctgtgg gtctacgtcc
     1921 gtgatgaccg taacgccggt tcacagatgc ccccggcggt ctggttcgcg tacagtccgg
     1981 accggaaagg tatccatcca caaaatcacc tggccggtta cagcggtgtg cttcaggccg
     2041 atgcttacgg tggttaccgg gcgttatacg aatccggcag aataacggaa gccgcgtgta
     2101 tggctcatgc ccggagaaaa atccacgatg tgcatgcaag agcgcccacc tacatcacca
     2161 cggaagccct gcagcgtatc ggtgaactgt atgccatcga ggcagaggtc cggggctgtt
     2221 cagcagaaca gcgtctggcg gcaagaaaag ccagagccgc gccactgatg cagtcactgt
     2281 atgactggat acagcaacag atgaaaacac tgtcgcgtca ctcagatacg gcaaaagcgt
     2341 tcgcatacct gctgaaacag tgggatgcac tgaacgtgta ctgcagtaat ggctgggtgg
     2401 aaatcgacaa caacatcgca gagaacgcct tacggggagt ggccgtaggc cggaaaaact
     2461 ggatgttcgc gggttccgac agcggtggtg aacatgcggc ggtgttgtac tcgctgatcg
     2521 gcacatgccg tctgaacaat gtggagccag aaaagtggct gcgttacgtc attgaacata
     2581 tccaggactg gccggcaaac cgggtacgcg atctgttgcc ctggaaagtt gatctgagct
     2641 ctcagtaaat atcaata
//
LOCUS       DAZFJF010000283         2648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFJF010000283 DAZFJF010000000
VERSION     DAZFJF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.285"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            170..349
                     /locus_tag="ACHKAU_005156"
     CDS             170..349
                     /locus_tag="ACHKAU_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5534017.1"
                     /translation="MIFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
     gene            390..635
                     /locus_tag="ACHKAU_005157"
     CDS             390..635
                     /locus_tag="ACHKAU_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5534018.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            713..928
                     /locus_tag="ACHKAU_005158"
     CDS             713..928
                     /locus_tag="ACHKAU_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5534019.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            933..1466
                     /locus_tag="ACHKAU_005159"
     CDS             933..1466
                     /locus_tag="ACHKAU_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5534020.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1741..2310
                     /locus_tag="ACHKAU_005160"
     CDS             1741..2310
                     /locus_tag="ACHKAU_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO5534021.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            2310..2459
                     /locus_tag="ACHKAU_005161"
     CDS             2310..2459
                     /locus_tag="ACHKAU_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534022.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            2467..>2648
                     /locus_tag="ACHKAU_005162"
     CDS             2467..>2648
                     /locus_tag="ACHKAU_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5534023.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDV"
BASE COUNT          697 a          570 c          761 g          620 t
ORIGIN      
        1 ggtggaagtg aacagcacag acactgcggc atgataaaaa aaaccgccag cgacaggaat
       61 ggacgctggc ggtggtgata cctatggaga aaaaataaag gaacgatact ttcgtactct
      121 ggtttttaat gaaaacagtt cttattgtca acaataacgg aaagaaatta tgatatttct
      181 gaaccagtta atgctgtact tctgtacggt ggtctgtgtg ctgtatctcc tttcgggtgg
      241 gtacagggca gtgcgcgatt gctggcgcag gcagattgac aaaagggccg ctgagaaaat
      301 cagcgccggt cagtcagccg gaagcaaacc cgaagagccg ctcatttagc ggcaactttc
      361 ttaatcacac ctttcgacga gaaaatccca tgtcagaaat tacatccctg gtcactgctg
      421 aagcagtgaa ggaagtcctg cgctctgaag aagtccggag cgcactgaaa cagaaacttc
      481 gccataacct ggaagcgcgt cttgatgcag aagtggatgc cattctggat gaactgctgg
      541 gcgcaccggc agctccggag ccggaaggca tcgcgggtga ggggagtgct tcagatagcg
      601 gtgaccccac accggacagc gacatgatga tgtaagcatg cgtcagggac catcggtgtg
      661 tgccggtggt cttttttatt gttgtgagct tccggattgc gggaggcggg gtatgaacca
      721 gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtgggaa cgggctactg
      781 gttcctgcag ttgctggaca gggtttcccc gtctcagtgg gcggcaatag gcgtgctggg
      841 gagtctgctg tttggtctgc tgacgtacct gacgaacctg tatttcaaaa tcagagagga
      901 caggcgtaag gcggcacggg gagagtaagc tgatgagcag gaaactccgc tatggtttat
      961 cggctgccgt tctggcgctg attgccgcag gtgcttctgc gcctgaaatc ctcgaccagt
     1021 ttctggatga aaaggaaggc aaccacacca cggcataccg tgatggtgcg ggtatctgga
     1081 ccatctgccg aggcgccatc atggtggatg gtaagcctgt gattcctggc atgaagctgt
     1141 cgaaggaaaa atgcgaccgg gttaacgcta tcgaacggga taaggcgctg gcatgggtgg
     1201 agcgtaatat aaaagttcca ctgaccgagc cacaaaaagc aggtatcgcg tcattttgcc
     1261 cctataacat tggccccggt aagtgtttcc cgtcgacgtt ttataagcgg cttaatgcag
     1321 gcgatcgcag gggagcgtgt gaggcgattc gctggtggat taaggacggt ggcagagact
     1381 gccgtatccg ttcaaacaac tgttacggtc aggtatcaag acgtgaccag gagagtgcgc
     1441 tggcatgctg gggtatcgac aggtaagcag aatattttgc tgaaaaatga cgttggccaa
     1501 cgcgggtaga caacacgaaa tcctgcgaac tggcaaaatg taagtgaata aagtcaacaa
     1561 gattgtttca tgaagaggca ccgtaatggt gcctttgtca tttctgcgct tcgcacaagc
     1621 gtaaataaac caaagaacct ttcaggatga gccctggtgg ataaccggca gtggtctggt
     1681 taaccctctt tgggctggtt attcctgtgc gcagggttca tcactaaaag gaaataaccg
     1741 atgaatatga tgaccgtgcc gtttcacggc gattctcttt atgtggttaa ccataacggc
     1801 gaaccatacg ttcccatgaa acctgtcgtt gcggggatgg ggctggcctg gcaatcacag
     1861 ttggctaagt taagacagcg ttttgcgtca actataacgg aaatcgttat ggttgctgag
     1921 gatgggaaac gacgcaatat ggtgtccctg ccgcttcgaa aacttgcagg ctggttacaa
     1981 accatcaatc ccaacaaagt aaaacccgaa atccgcggca aggtaatcca gtatcaggaa
     2041 gagtgtgacg atgttctcta tgaatactgg acgaagggtt ttgtcgttaa tccccgtcga
     2101 atgagtgtga tggaagaact caatcaggct tgcgctgaca tgaaacggga taaaaacatt
     2161 gccagtgtgt ttgctaccgg gctgaatgag tggaaacagg ttaaagccgc gcatgtatca
     2221 aaaatccgca cattgataaa cgaagcgaat ctgctgattg attttgtcct ggctgataca
     2281 gacaaaggga aaataacaaa ggcggattga tggagtggtg gctaatgata tcggataaac
     2341 tcataacgct ggcgaagatc ctctgtgtaa tcgtcggcat ttcattttta gtcattctgg
     2401 ttgccatttt cttttccacc gcttggcgag tcctgacgtt atcgggactg gtggggtgaa
     2461 agagagatga accgtgttct gtgtgtggtg attattgtcc tgctggtagc ctgtggtgca
     2521 cttagtctgg ggctgaatca ttaccgcgat aacgccataa cctacaaaga gcagcgcgat
     2581 aaaaaagtca gtgagctgga gctggcaaat gcaaccatta ctgatatgca gcagcgccag
     2641 cgtgatgt
//
LOCUS       DAZFJF010000284         2646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFJF010000284 DAZFJF010000000
VERSION     DAZFJF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.286"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..656)
                     /locus_tag="ACHKAU_005163"
     CDS             complement(51..656)
                     /locus_tag="ACHKAU_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO5534024.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            complement(758..916)
                     /locus_tag="ACHKAU_005164"
     CDS             complement(758..916)
                     /locus_tag="ACHKAU_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534025.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTEAHVH
                     AESSLVSP"
     gene            complement(913..1065)
                     /gene="kil"
                     /locus_tag="ACHKAU_005165"
     CDS             complement(913..1065)
                     /gene="kil"
                     /locus_tag="ACHKAU_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5534026.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            complement(1050..1181)
                     /locus_tag="ACHKAU_005166"
     CDS             complement(1050..1181)
                     /locus_tag="ACHKAU_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO5534027.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(1206..2174)
                     /locus_tag="ACHKAU_005167"
                     /pseudo
     CDS             complement(1206..2174)
                     /locus_tag="ACHKAU_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            2430..>2646
                     /locus_tag="ACHKAU_005168"
     CDS             2430..>2646
                     /locus_tag="ACHKAU_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFO5534028.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGM"
BASE COUNT          559 a          644 c          532 g          911 t
ORIGIN      
        1 ctgaaactct cttgcgggct tgtttagtct tacagtaatc gaatgtgcca ttaagcagca
       61 gctccttcta attcgtctcg tctgatgttg taaacgtcct gcgctttgtg ctgctccggt
      121 gtgccttcga gcatcttcca cgctttggcg aacgcctgtt taagctcttc tacggtgttt
      181 ttctgcattg ctgcgtcagt gaatgctttt agaacctgtt caggtgtagg tgatggtttt
      241 gattgctttg ctgctgcgtt ctgctgatgt ttatgctcgt cggtatctgc atctttcgca
      301 tcatcaatgc cgaataaacc attgaggcaa tacttgcgtg cataagagct tgtagctccc
      361 gtaacttgtg cagaatccat tcctttcttg ctttcttcct ctcgtgcaag agcggttgcc
      421 gtatgactgt tttcgccatc ggtaatagtt gccgtggctt tcacataata ccgatcacca
      481 atcaacacaa cttcatcgct gattgataaa aacaggccat tcagtaacgg cttaacgcct
      541 tcaagaatat cttcgcagct tctgtattta tatttgccga atgagttgta ctgattcttt
      601 ggcgcgttca gattctcctg aatagctgcc agtcttgcgt aaaattcttt gctcatatga
      661 ttgttctcag aatggacacg gcccaaggaa ataacgctga tttaatactt cgactcggga
      721 caaattaagg catacccgca ttccttcgcg gtcaccatta tggcgatacc agagagcttt
      781 ctgcgtgtac atgcgcctct gtaacttgct ctccttcact gtggttgcaa gtgacatgaa
      841 tatctccttc gttaccgatt aattctttca tctgacgaat gaattcttcg tctgaccagt
      901 tatctgtaaa actcatttcc tgcgataaca cggaaggttg atagctgatt tcatcgcttt
      961 atttgcttca agccacattt ttgaatcacc aataaatcgg gctattactg ccttgttctg
     1021 tgcagcacga agcatctggt gattgatggc tatttcattg cgcataataa gacctcaact
     1081 cttttccatc cgtcacgtaa tttacgggtg attcgttcaa gtaaagattc atttaattgg
     1141 aaggcaccca tgcgagcgcc tcccgcgatt gcgtaaatca tgggtggttc cttatgttgg
     1201 ttttattagt aggttatttt tgttgcgaat aattcgcctt ttacgatggc tgttatgata
     1261 tttttagcaa catcttctga tgcgccaacc ttgataaggt cagcaagtat tttgttattt
     1321 acttctttcc ggtgagcttt atcctttgct ctacgctctt cttcgtcctt gattcttttt
     1381 tcttctgcta ttctggcttg ctcttttgct tcagcctcgc gcaggattcg ttctgcctcc
     1441 tcctgtgctt tacggcgttc tgcttcaatt gccgcatgct tttctctttc agctcgttct
     1501 gctgcctctt ttgcttcgcg ctgtgctcgt tgctcggctt caatgcgttc acgctctgca
     1561 cgttcctctg cggccttagc ttctgcttct cgccttgctg ctgcttcaat ttcggctttt
     1621 gcctttgctt cggcttcagc tctggctttc tcttcagctt ctctttttaa gcgttcttca
     1681 tgctctcgct tttcctgctc cgctttgagt cttgcctctt ctctttggcg gtcaaattcg
     1741 cgatccatca aaatcgctat ttcatggtca gactcaattt gctttgcgag agcttcagct
     1801 gctgccttag cttcttcttc ggctttaatc cgcgcctgtt cttcctcata atcagtaaga
     1861 ggctggcgcg ccttggcttt cagttcatca aggcgatcgc gcactgtctt gcggttagca
     1921 tcaattagct ttggaatttc cttcagttca gcaacaaggt ctttgccaag accatcgaga
     1981 tatgttttcg tctgcgcaac tttatacgcc agataagcga tctcctttct gccctttgcc
     2041 gttgtgatat caggcacaaa ggacataact tcacgttcaa ccttttgaag aatttcttca
     2101 atctggtcgg cagactaaat tacagtcatt gcatttgctt tttcaataac aactaaatct
     2161 gttacttcac tcatatatcc tccgtcaaaa aaattgccct cacactggag ggcaaagaag
     2221 atttccaata atcagaacaa gtcggctcct gtttagttac gagcgacatt gctccgtgta
     2281 ttcactcgtt ggaatgaata cacagtgcag tgtttattcg tatgcctgtc ttttaaccac
     2341 atcaggctcg gtggttctcg tgtaccccta cagcgagaaa tcggataaac tctattcacc
     2401 cctacagaga gaatgatgga gattcaccga tgagtaactg gtggcaggaa ctattacgtt
     2461 tcttcctgcg tggtcttacg ctacaacagt taattcatat gcttattatt ttaattgcct
     2521 tgataataat caccccagca tcaattaaag agtgggtaga tataaggaac cccgaaatac
     2581 ttccagatca ctggatgtat tacgcaatgc ttttgtgtat cagttatgtt ctgaacaggg
     2641 ggatgg
//
LOCUS       DAZFJF010000285         2640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFJF010000285 DAZFJF010000000
VERSION     DAZFJF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.287"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..269
                     /locus_tag="ACHKAU_005169"
     CDS             <1..269
                     /locus_tag="ACHKAU_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5534029.1"
                     /translation="HGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSSKKID
                     LNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
     gene            complement(362..721)
                     /locus_tag="ACHKAU_005170"
     CDS             complement(362..721)
                     /locus_tag="ACHKAU_005170"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5534030.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1407..1697
                     /locus_tag="ACHKAU_005171"
     CDS             1407..1697
                     /locus_tag="ACHKAU_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5534031.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            1721..1972
                     /locus_tag="ACHKAU_005172"
     CDS             1721..1972
                     /locus_tag="ACHKAU_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5534032.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(2020..>2640)
                     /gene="traT"
                     /locus_tag="ACHKAU_005173"
     CDS             complement(2020..>2640)
                     /gene="traT"
                     /locus_tag="ACHKAU_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO5534033.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          734 a          534 c          567 g          805 t
ORIGIN      
        1 accatggaga atctctggga gaaaatggat tatacttaca tggaacacca tataatgttg
       61 ctccagctga gcaaacgcat gtgcctctta ttacctggat gtctccgggg ttcgtttcct
      121 ctaaaaaaat tgatttgaat tgtttgtcag aacatgctct aaacagaaca gtaagtcatg
      181 ataatatatt ttcatcgtta ctggggttat ggaatgtaaa tacttcagtt tataataaag
      241 aagatgatat tatatcctcc tgcagataga tactgacatt tttctgagga taagaggtga
      301 atacctctta tccttaagta aatattaacc aggtaactgt gaataattta gatgttgggc
      361 gttatgaaag taataaaact ccccatataa tgatagcaac taaaatcgta atcaacactg
      421 cggctgaacc catatctttt gctctgcctg ataattcgtg atgttctgaa ccaattctgt
      481 caacaactgc ttcaattgca ctgttaatta tttcaacaat aattaccatc attgccgaac
      541 caatgagtaa tactcgagta acagagtcaa catccagcca aaaggatatt atcagtgctg
      601 acagaaccag aatgcactcc tgacgaaagg ctgcttcata gcgccatgcc gcacataagc
      661 ctttccatga ataacccgct gctttaataa ttctggtaac accagtggtc gtattagcca
      721 taaatacctc ttatttaatt atatgtctga atatgcatta taaaatatca gttaacatta
      781 acaggatttt gtaataagcc acatttattc tttcgggaat taaaaaacat aacgaaattt
      841 tgcctcattc tgcatttcat tgaaatagta tgtttctcat cgaaagcaag tttgtcaata
      901 taaatttgta acagaaatga ctttccgggg ataaacaaaa ctgttcatgg caatggcttt
      961 ctctgtcgcc aacatggata cattgttccg gataaagcac tgatgtcatt gactgacgca
     1021 gactatcagg ccatctgatg ctttcttttc cttcaccggt attgatgata tgccttatga
     1081 agggggactt attcctgtaa cccaatcaca ggcactgtga ttactggttt atgattgatg
     1141 ctgatttgag cagatatgca cagcagttgt ggattcaggg gatattttct gccagatatt
     1201 cataccagat tgatgacctc tttgctcaga tgctcttcgt tctgagccag ccaggatttt
     1261 gtggccacat acattgttcg ccgatgggga atcgagacag gcgattggct gtgatgcgat
     1321 tttgccgcta actgaggatg agtccgcccc tcccggggaa ttcctgtaag ttaagccgta
     1381 cctgttgatt agcactaaac tgggtaatga atttatctgc cagtatgaat gatgccgggg
     1441 acggttttga actcctgatg tcgggtaata tgcaggattt taatatttgt tgtcgtcggg
     1501 ataatcatta tcctgaacat aaactgatac ctttatcaga gttatttgac gttaattatg
     1561 gactgaatct tgagctgaat aaactggaga aagattcttc aggaattaat tttgtttcaa
     1621 gaacgtccaa aaataatggc gtctccgcca gggttaaatt aaaagatggt attagtccat
     1681 gccagcagct gtgttaactg tagcagcagg aggttctgtt atggaatcgt ttgtacagga
     1741 ttcaccattt tatagtggaa gagatcttta ttggttacgt cctaaagttg aactgactct
     1801 ggaagagaaa ttgtactact gctcttgtat aagaagaaac agacacaaat acagctacgg
     1861 gcgtcaggca aaccgaacgc tgaagaattt actggtaccg tcattagatt ctgttccggc
     1921 atgggtatat ggtgtgacag ggaaaataat atctgaattg tctgaacgtt gagactaaga
     1981 gggccggagg ccaggtgaat ggccaccggg atatttatat cagagtatat tggcgattga
     2041 ttttgccagc tggtcttcca gatgaggttt agcttcttca aacttcagat taacctggtt
     2101 ggcactggat acaacccggg tctggtattt atgctggttg ccggtttctg tgcttgtctg
     2161 aattttggag cctgatgttc cctgacgcaa agctgcaaca ttatccgttc tgacgctggt
     2221 tctggttcgc tcggcaatct gaacatctgt aatcattgtg taatttatat cttccaccat
     2281 tgcatccgcg gccatgccaa tcagaccggt agccagtcca acaccgagag tggtacctgc
     2341 cgacccggta ttgtacgctg tgattccacc tccaagagca gctccgactg ctgcaccttc
     2401 ataaccgcga ctcagccatc cctgagattc acgcagatcc attttgtctg ctttgagaac
     2461 gttagcctgg atccagtagt aagctttatc cggggaagtc atgatctgat aacctctggc
     2521 tttgactgca tcggcaattt ttccctgtag tccactcatg tctttatccg acgtgttttt
     2581 gatctgcagg aaaacggtgc gttcgctcgc aggttcaagc cagatggttg aggtgtactg
//
LOCUS       DAZFJF010000286         2632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFJF010000286 DAZFJF010000000
VERSION     DAZFJF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.288"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..369
                     /locus_tag="ACHKAU_005174"
     CDS             <1..369
                     /locus_tag="ACHKAU_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO5534034.1"
                     /translation="TDKDLKTDPPLNPPRGNRGVKKFDPLDIALPNWISVSLWREWVE
                     FRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETL
                     LRQVNTVSLPDSAIPPGFRG"
     gene            376..1122
                     /locus_tag="ACHKAU_005175"
     CDS             376..1122
                     /locus_tag="ACHKAU_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5534035.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEAQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIEK"
     gene            1145..1906
                     /locus_tag="ACHKAU_005176"
     CDS             1145..1906
                     /locus_tag="ACHKAU_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5534036.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPMQAELPEETTTATEAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIVDSSSRIVSEK"
     gene            1922..2344
                     /locus_tag="ACHKAU_005177"
     CDS             1922..2344
                     /locus_tag="ACHKAU_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5534037.1"
                     /translation="MAKVFTQEEREKIKEQVVELVRQSGRETLRQLEAKTGATRYLMS
                     ILARELVASGDVYNSGYGLFPSEQARKDWKNARKKLSRAKVKKTVVVDPDLIWSLPDG
                     EIRRYDRRQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            2341..>2632
                     /locus_tag="ACHKAU_005178"
     CDS             2341..>2632
                     /locus_tag="ACHKAU_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO5534038.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVA"
BASE COUNT          717 a          577 c          776 g          562 t
ORIGIN      
        1 acagataaag atctaaaaac agatcccccc ctaaatcccc cccgggggaa tcgaggtgtc
       61 aaaaagtttg accctctgga tattgctttg ccgaactgga tttctgtctc gctttggcgt
      121 gagtgggttg aatttcgcca ggcattgcga aaaccgattc gaacggagca gggcgctaac
      181 ggggcgatac gggagctgga aaaattccgc cagcagggtt tttcacctga gcaggtgatt
      241 cgacacagca tcgccaatga ataccagggc ttgttcgcgc cgaaaggtgt tcgacctgag
      301 acgttactcc gacaggttaa caccgtctcg ttaccggata gtgcgatccc gccaggcttc
      361 agggggtaac tgaccatgaa aaatattgcg acaggcgacg ttcttgaacg tatccgcaga
      421 ctggccccgt cacatgtaac cgcgccattc aagacggtag cggagtggcg cgagtggcaa
      481 ctttccgaag gccagaaacg ttgtgaggag atcaaccgtc agaatcgtca gttgcgggtg
      541 gaaaaaattc tgaatcgctc tggcatccag ccattgcacc gcaaatgctc gttttcgaat
      601 taccaggtgc agaacgaagc gcagcgatac gcgttgagtc aggcgaaatc catcgctgat
      661 gaactgatga ccgggtgtac aaattttgcg ttcagcggaa aacctggtac cgggaagaac
      721 cacttagcgg cagctatcgg gaatcgcctg ctgaaagacg gtcagacagt gattgtggtt
      781 accgtggctg atgttatgag tgccctgcac gccagctatg acgatgggca gtcaggcgaa
      841 aaatttttgc gggaactatg cgaagtggat ctgctggttc ttgatgaaat tggcattcag
      901 cgcgagacga aaaacgagca ggtggtgctg caccagattg ttgatcgccg gacagcgtcg
      961 atgcgcagcg tggggatgct gacaaacctg aactatgagg ccatgaaaac attgctcggc
     1021 gagcggatta tggatcgcat gaccatgaac ggcgggcgat gggtgaattt taactgggag
     1081 agctggcgtc cgaatgtcgt ccagccagga attgagaagt aatttttacc gggaggaaat
     1141 ttttatggag actgtttttg acgcactgaa agcaatggga aaagccacat ccatagaact
     1201 tgctgcgcga cttgatatca gtcgtgaaga agtgctgaac gaactatggg aactgaaaaa
     1261 agctggtttc gttgataaaa gcgcgtacac ctggcgtgtg gctgataaca acgttcagca
     1321 ggaacagcca atgcaggcag aactgccgga agaaaccacc acggcaacag aagcgaaaat
     1381 ctcagagtgc gatttaaccg cgacgattga acaacgcgga ccacaaacgg cggatgaact
     1441 ggctacgctg ttcggtacaa catcccgcaa agttgcttca acgctggcaa tggcaatcag
     1501 caaaggtcgt ctgattcgcg taaaccagaa cggtaaaatt cgttactgca taccaggtga
     1561 taatttacca gcagagccga aagttgaatc ggtagcggaa actgatggta aagcctttcc
     1621 tcagccagcc ggtgttgcgt taccagtaca ggaagcggca acacaggaag atattaaaac
     1681 agaaactgtg gcggacattg tgcagtcgct gccatcgttt actgaaacgc aagcggatga
     1741 cctagtttta ccatcgctgc atatggcaaa ccgcgaactg cgtcgggcga aaaatcatgt
     1801 ccagaagtgg gagcgagtct gcgccgcgct gcgggagctg aacaagcacc gggatattgt
     1861 ccgacagatt gtcgattcct ccagtcgtat tgtgtcggaa aagtgattgc cggaggcgct
     1921 tatggcaaaa gtttttacac aggaagagcg tgaaaaaatt aaggagcagg ttgttgagtt
     1981 ggtacgccag agtgggcgcg agacgttaag gcaactggag gccaagacag gtgcgacaag
     2041 atatctgatg agcattcttg ccagagagtt ggttgccagt ggcgatgtat acaactctgg
     2101 ctatgggtta ttcccgtctg aacaggctcg taaggattgg aaaaacgccc gcaaaaaact
     2161 atccagggca aaggtgaaaa aaacggttgt ggttgatcct gaccttatct ggtcattacc
     2221 agacggagaa atacgccgct atgacaggcg tcagaacata atttgtcgtg agagcaggag
     2281 gagcgaagtt atgcagcgtg tgctggcgtt ctatcgggga aactttcagg aggtgatgga
     2341 gtgagggtga gagtttatat tgccggtcca atgacgggat atgaaaattt caaccgtgag
     2401 gcatttcaca aggtggaaga ggaactgaaa cgggaagggc ataccgtctt aaacccggca
     2461 gtacttccgg acgggctgac acagccgcac tacatggata tttgcatggc aatgattcgt
     2521 tgtgtggatg cgatttacat gctgaatggc tggcagcggt cagcgggcgc taaggcagag
     2581 ctggcactgg cggagaaact ggggcatgcg gtgatttatc aggaggtggc tc
//
LOCUS       DAZFJF010000287         2607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFJF010000287 DAZFJF010000000
VERSION     DAZFJF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.289"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..185
                     /locus_tag="ACHKAU_005179"
     CDS             <1..185
                     /locus_tag="ACHKAU_005179"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5534039.1"
                     /translation="RYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAK
                     YVSVIWQKLIPSQADS"
     gene            258..509
                     /locus_tag="ACHKAU_005180"
     CDS             258..509
                     /locus_tag="ACHKAU_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5534040.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLA"
     gene            529..1824
                     /locus_tag="ACHKAU_005181"
     CDS             529..1824
                     /locus_tag="ACHKAU_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO5534041.1"
                     /translation="MREAEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     IAGELRASVCYAIKTGAFDYAKQFPASHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMIIRRNLAKDRFTVPKTQAGTNR
                     VIHLIKPAIDALRSQMALTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSAKVKNYG
                     DHFTVDSIRQMWDAAVKRAGIRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSENNNAQVTLLNTLLSEFAPTMPHNEEMKS"
     gene            2472..2585
                     /locus_tag="ACHKAU_005182"
     CDS             2472..2585
                     /locus_tag="ACHKAU_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO5534042.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
BASE COUNT          815 a          555 c          549 g          688 t
ORIGIN      
        1 ggcgttacta caacgatcgc gatgtgcgca caatcgttga gctggggaaa gccatagact
       61 tcgatgccag aacggctatt ccattcgaag gtgagcgcca taatgcactt gatgacgccc
      121 gttaccaggc aaaatacgtt tcagttatct ggcaaaaact gatcccgagt caggctgatt
      181 cttaatgttc aactgtcgcc ggttgtgact ggtattctgc aaccggcgct cgtctgatgt
      241 aagagataaa gaaagcgatg agcgaagtaa tcatgattgt ctctcccggc aaatgggtat
      301 ccgaagagca gttaattgcg ctgaaaggaa taaaaaaagg tacgttaaaa aaggcccggg
      361 aaaaatcgtt tatggaagga agggaatata agcatgtcgc tcatgacggt atgccatggg
      421 ataacagtcc atgcttttac aacctggaag aaattgatcg ctggattgag cgccaggcat
      481 ctgcaagacc aagacgtcat cttgcttgac taaaagcaac acgaaccaat gagagaagct
      541 gaaatgaaat atccgacagg cgtggaaaac catggaggga aattacgtat ctggtttgtt
      601 tataaaggtg taagagtccg ggaaaatctt ggggttcctg acacagcaaa aaacaggcgc
      661 attgcaggtg agctacgcgc ctctgtttgt tacgcaataa aaactggcgc tttcgactat
      721 gcaaaacagt ttcccgcctc acacaatctg gaaaaatttg gtgaggcccg acaagattta
      781 accataaaag aactggctga aaaatttctg gcactgaaag aaactgaagt cgcaaaaacg
      841 tcactcaaca cgtaccgtgc cgtcatcaaa aatattctga gcataatcgg tgaaaaaaat
      901 cttgcatcat cgattaataa agaaaaattg ctggcggtac gtaaagagtt actgactgga
      961 taccagatcc ccaaaagtaa ctatattgtt acacaaccag ggagatcggc tgttaccgta
     1021 aataattaca tgacaaatct ttacgccgtg ttccagtttg gtgttgataa cggttatctg
     1081 gcagacaatc catttaaggg gatctcacca ttaaaggagt cgagaaccat cccggatcca
     1141 ctttcgcggg aggagtttat ccgccttatt gacgcgtgca gaaatcagca agccaaaaat
     1201 ttatggtgtg tttccgttta tactgggatt cgccctggtg aactctgtgc gcttgggtgg
     1261 gaagatatag atctgaaaaa tggaacaatg ataatcagaa gaaatctggc aaaagaccgt
     1321 tttacagtac caaaaacaca ggcgggaacc aatcgtgtga ttcaccttat taagcccgca
     1381 atcgacgctc tccggagtca gatggcacta acgagactga gcaaagagca tatcatcgat
     1441 gttcacctca gagaattcgg cagaacagag aaacaaaaat gtacctttgt ttttcaacct
     1501 gaagtgtcag cgaaagtaaa aaattatggt gaccatttta ccgttgactc aataaggcag
     1561 atgtgggacg cagcggtaaa gcgtgccgga atccgccatc gcaaatcgta tcagtcgaga
     1621 cacacttatg cctgctggtc gttaacagca ggagctaacc cggcattcat tgcaaaccag
     1681 atgggccatg cagatgcgca aatggtgttt caggtgtacg ggaaatggat gtcagaaaat
     1741 aataatgcgc aggtaacgct gttaaataca ctgttaagcg agtttgcccc aaccatgccc
     1801 cataacgaag agatgaaaag ttaatttttt atttatcaat tagttagatt gaatgactct
     1861 tgaaatccat attcacaggt gttttttccc atcctgtggt ttccttggcg ttttctaggt
     1921 tttttcagat agttgcattt ttttaaaagc atcctaagtt cgatctcagt gtctatctgg
     1981 ggcctatttc tgtcccatat atgccccaaa aaactcccca acagataagt agttttttca
     2041 tggatttacg cgtaaaatca ataacggctg gaaatcattc aatactcgca ctatcgaaaa
     2101 atttaccagc caaccgcagc acgttcttgc ataaggtgtg tctgcggttt ttcaactatt
     2161 cagatacatc actcccatca cattcattcc tccacatcaa aggcatatag gctatatcac
     2221 cttgatattt ttctttttca gataaaaact gttatctatg tatactttta aacccaatcc
     2281 gtgtagagtc tctgcataag atagtttgca gttgccgctt cagcttgcgc cataaaccgc
     2341 ctgatttttg ctgccacctg ttagcattcc tgtatacctg aaacgacaat gtttatctac
     2401 gaactttaag aacacccaag ataaaaattg tcaactatat catatataac acattactaa
     2461 ttcgaggcta tatgaacagc atactgataa tcacttctct ccttatcata ttcagcattt
     2521 ttagtcatgc cctaataaaa ttagggattg gcatatccaa taacccagac aaaaccgatg
     2581 tataagtcaa catatcctga atcagac
//
LOCUS       DAZFJF010000288         2578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFJF010000288 DAZFJF010000000
VERSION     DAZFJF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.290"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..330
                     /locus_tag="ACHKAU_005183"
     CDS             <1..330
                     /locus_tag="ACHKAU_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5534043.1"
                     /translation="LASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAF
                     RFAGNNSLQVIPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVE
                     VKKAVTE"
     gene            477..659
                     /locus_tag="ACHKAU_005184"
     CDS             477..659
                     /locus_tag="ACHKAU_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5534044.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            695..940
                     /locus_tag="ACHKAU_005185"
     CDS             695..940
                     /locus_tag="ACHKAU_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5534045.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(979..1445)
                     /locus_tag="ACHKAU_005186"
                     /pseudo
     CDS             complement(979..1445)
                     /locus_tag="ACHKAU_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1560..1760
                     /locus_tag="ACHKAU_005187"
     CDS             1560..1760
                     /locus_tag="ACHKAU_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5534046.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            1714..2451
                     /locus_tag="ACHKAU_005188"
     CDS             1714..2451
                     /locus_tag="ACHKAU_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO5534047.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESIFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRANVHKFRQFRYIRFLNKRAK
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
BASE COUNT          772 a          564 c          593 g          649 t
ORIGIN      
        1 ctggcatcgt tctacatcca gacagacgca aaagacctga atgtgatgta ccacaatgca
       61 aaagtggcaa caaataacca gaaactggga acctttggtg catttgataa tgaatggcat
      121 acgctggcct tccgttttgc aggaaataac agccttcagg ttattccggt tattgatggt
      181 caggatggtg ccgcgtttac cctgacgcag tcaccagtag gaactttccc ggtggacaaa
      241 ctccgcgtaa cggatatcac caaaaatgca acttacccgg tgctgattga cagcattgtg
      301 gtggaagtga aaaaagcggt aacagaataa aaaaatcccg ccggtcatga tatggctggc
      361 gggagctacc taacccataa aggaaaataa aaatactccc gaaggagtgg ttatcagtcg
      421 cttcgatgtt tttacccgat aacgaattat gtagtcaata tcaaaggtaa ggttatatga
      481 cattcgttca tacaatgctg ctttatttct gcgctgtggt cagtgcgtta tatctggtga
      541 gcggcggtta taaggtcatc agaaattata ttcgccgtaa aattgatgat gcggcagctg
      601 aaaaactcag taaaacagca caggcaccgt catccccaaa cgacccgacc ccgctctgat
      661 aacggggcaa tatacctgca aaaggagaat attcatgcca gagattaaag gcacggttac
      721 tgaagagctt gtcaaacagg cactttattc tgaagaagtg aaccgcgtgc tgaaggcgca
      781 ggttcgcaag gattttgagg cacaaatcga cgcatatgtt gatgaagtgc tggcccgaat
      841 ggttggccgt tctccggctg aaaacagcac ggaaaatgat cctcaacccg tagagcaacc
      901 agagccggtt cagcccggaa ctgacggtac tatgatgtaa caataccgac cggcaggttt
      961 ccctgccggg tgagtttgtt aatgcacgtt ttgtgtattt tccctcctgg gagaacaggc
     1021 aggacatatc agcatcctga aacaggaagc atttttgggt ttgtatgtct tgccattttg
     1081 agatcgagaa cggcgataga tgagctgtaa gggagtctta caggcagggc aaacaggctc
     1141 tttcatccat gataaagatc tcctttttat gcatctctgc tttacaggag actggggaat
     1201 atcataggat acgccacgag ctcgaagtaa agcattgatg caaaattgtc taaatcggcc
     1261 cagaatagtt cgcgctatct gggtcgctct ttatgtatgt agtatcccat tgatttgaaa
     1321 catactacat atacgcgcga tcctaccttc actatataaa aagtcaataa aatgatcatt
     1381 ttatgatcgt tttcaccacc taactcttcg ttttctttaa gtaaataatc acctacctga
     1441 atcattgttg atgacttttc aatctatttg ttgtatcaat taaacaaatc gaatcgatcg
     1501 tttttgtcga tcgattgcat gagaaaggca tcaacaacaa agcaggaggt tatgcggcca
     1561 tgtatcgaaa catacggtgc cgacactgca ataagctact ggcacgggcc agtttcagtt
     1621 atctggaagt aaaatgtccg cgctgtaaaa ccctcaatca aatcacatct ccgagcgcca
     1681 cagagcaccc cacatacaca aggaaatcct gtcgtgggga acagaaagca agtcacatcc
     1741 cgaatcatca gcacgcctga acttattcgc tataacgaca atatcgttgg ttatggctcc
     1801 cgtgagttgc gggttgagac aataagttgc tggctggcca gactggtcat cgtcaataaa
     1861 cattacagcc accgtttcgt aaataactcc taccttcatc tgggcatatt ttcagaacgg
     1921 gaacttgttg gcgtaatgca atggggttac gcccttaacc caaacagtgg tgcacgcgtc
     1981 gtaacgggta cgcagaaccg agaatatatg gagcttaacc ggctatggct gcatgactgt
     2041 atgccgagaa actccgaatc aagggcaatc agttacgcgc tcaagctaat caggcagctt
     2101 tatccgcagg tacagtgggt tcagtcgttt gcagatgagc gttgtggctg tcttggtgtc
     2161 gtgtatcagg caagcaattt tgattatgta ggcagccatg aatcaatatt ctatgaactt
     2221 gatggcgagt ggtatcacga gatttgcagg aatgccatta aacgaggcgg acaacgaggt
     2281 gaacatctga gggctaatat tgacagagcc aatgttcata agttccgaca gtttcgttac
     2341 atccggtttc tgaacaagag ggccaaaaaa cgcctcaaca caaagctatt caaagtccag
     2401 ccttacccaa aaccacaaac agttaaaact ggtttgaaag agagtgaatg agcgcttaaa
     2461 aactcattct aaaactcaaa agaaaagcgc cgatttagta tcaaataagt tgcgtatcgg
     2521 cgtttttcag cagtatcaaa tgaaatgcaa atcggtatca aaaaaatcgc cgcgctat
//
LOCUS       DAZFJF010000289         2562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFJF010000289 DAZFJF010000000
VERSION     DAZFJF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.291"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..347)
                     /locus_tag="ACHKAU_005189"
     CDS             complement(30..347)
                     /locus_tag="ACHKAU_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5534048.1"
                     /translation="MSKNYRSDALASVHEMMESLHDIGAITKQTMREFDETCLQPAPV
                     MSPERIRALREREHLSQPVFARYLNVSKNLISDWERGVKRPGGAALRLLSVVEKNGIQ
                     VIS"
     gene            complement(340..711)
                     /locus_tag="ACHKAU_005190"
     CDS             complement(340..711)
                     /locus_tag="ACHKAU_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5534049.1"
                     /translation="MRIFKNAWFERFARKHRISDKSLRKIVEQADKGIISANLGSGVI
                     KQRLARSGGGKSGGYRTIIFYRVAEKAFFIYAYAKNERENITAIEENAFRKAAPHVLN
                     LTDEQLAQLIQQGQFTEVPNE"
     gene            968..1162
                     /locus_tag="ACHKAU_005191"
     CDS             968..1162
                     /locus_tag="ACHKAU_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534050.1"
                     /translation="MKIPASLIPGFYESTRPVVVYRNSDGSFQCGFVMRSNEVVVSLS
                     LLSEVRELAGLPVDDIQKQL"
     gene            1216..1485
                     /locus_tag="ACHKAU_005192"
     CDS             1216..1485
                     /locus_tag="ACHKAU_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534051.1"
                     /translation="MAHDSRLHNSAALASRQGRCSHVFKTDWFQHPLCTEEQAEWLIQ
                     CYRRRGYEFQKDLSFDRRHWIISARLPYSERPPRPSRTFQQRIWR"
     gene            1487..2536
                     /locus_tag="ACHKAU_005193"
     CDS             1487..2536
                     /locus_tag="ACHKAU_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5534052.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSVFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPSHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
BASE COUNT          634 a          614 c          645 g          669 t
ORIGIN      
        1 gcttcggcgg gttttgttgt attagaatat caggatatta cctggatccc gtttttctcg
       61 acaactgaaa gaagccgaag agctgcacct cccgggcgtt tcactcctcg ttcccagtct
      121 gatatcaggt ttttactgac gttgaggtat ctggcaaaaa caggttgaga cagatgctct
      181 cgttctcgca gtgcacggat cctttccgga gacattaccg gcgcaggctg aagacaagtt
      241 tcatcgaatt cgcgcatagt ttgttttgtg attgcaccga tatcatggag tgactccatc
      301 atttcatgta cagatgcaag tgcatcactg cggtaatttt tactcattgg gtacctccgt
      361 gaactggcct tgttgaatca attgtgccag ctgttcatca gtaagattga ggacatgggg
      421 agcggctttt cgaaaagcat tttcctctat agcagtgata ttctctcgtt cattctttgc
      481 gtatgcgtag atgaaaaagg ctttttctgc aacgcggtaa aaaattattg tccggtaacc
      541 gcctgatttt ccgccaccac ttcgggctaa tctttgttta atgacaccac tacccaaatt
      601 tgcggatatg atccccttat cggcctgctc cacgattttg cgcagcgatt tatcggaaat
      661 ccgatgtttt cgagcgaaac gttcaaacca ggcgtttttg aaaattcgca tacctgcctc
      721 tgcattttat ataacacata gtgttacacc atgtggacta tggaggcaat ctttatttga
      781 gaatgcttgc tgatttatcg gtgttcaaaa aacagacgta attcagattg tataaacggc
      841 agacagaaaa agtcattgtt cttgcgcata aaatcctcag atttactgag tgaaatgcgt
      901 ctcttttcat ggttatagtc cgagagaagc gataatgcat ttcatttcct ttacgtcagg
      961 aggtgttgtg aaaatcccag ccagtttgat cccggggttt tatgagagca cgcgcccggt
     1021 tgttgtttac agaaacagtg atggttcttt tcaatgtggt tttgttatgc gaagtaatga
     1081 ggttgttgta agcctttctc ttctttctga ggtaagagag ttggcaggat tacctgttga
     1141 tgatattcag aagcagctat aaaatcaggc tgacttgaac cgtcgctgag aaacaccgtg
     1201 ccaccggaga tcccgatggc gcacgatagc agattgcaca attctgccgc ccttgccagt
     1261 aggcaaggac ggtgttcgca cgtatttaaa accgactggt tccagcatcc cctatgcact
     1321 gaagaacagg ccgaatggct gatccagtgt taccgcaggc gtggttacga gtttcagaaa
     1381 gatctcagct tcgatcgtcg tcactggata atctccgcca ggctccctta ttccgaacgc
     1441 ccaccgcgtc cgtcccgcac attccagcag cgcatctgga ggtaacgtgc gggtattact
     1501 tcgacctgtt ctggtaccgg aactcggtct ggttatcgtt aagccaggcc gtgaatcaat
     1561 gtcagtattc cataacggca gaatattggt ggagccggaa ccgaaaaaca tgcgcggtct
     1621 gccgtccgga gtcgttcctg ccgttcgcca gccgctggca gaggataaaa cattactgcc
     1681 atttttcagc gatgagcggg ttattcgtgc agcaggtggt gcaggtgcac tgtctgactg
     1741 gttattacgt cacgtgaaat cctgccagtg gccacacggc gattatcatc acagcgaaac
     1801 agtcattcac cgttatggta ccggcgcgat ggtgttgtgc tggcactgtg acaaccagct
     1861 gcgcgaccag acatcagaat cactcgagca acttgctcaa caaaacctgt cagcatggat
     1921 gattgacgtc atccgccacg caatgaatgg catacaggaa cgggaattat cgctggctga
     1981 attatcctgg tgggcggtcc gcaatcaggt ggcggacgcg ctaccggaag cggtattacg
     2041 tcgttcgctg gggttgcgtg cggaaaaaat ccgctccgta taccgtgaaa gcgacatcat
     2101 accgggagaa cagaccgcca ccagcatact gaagcagcgc acaaaaaata ttgcgctacc
     2161 gtctcacacc caccagcaac agaacccacc acaggaaaag acggtggtca gcattgccgt
     2221 tgatccggag tctccggaat ccttcatgaa acgacctaaa cgtcgccgct gggtaaatga
     2281 gaaatacaca cgctgggtaa agacacagcc gtgtgcgtgt tgtggtaagc cagccgacga
     2341 tccccatcac ctgattggtc atggtcaggg cggaatgggg acaaaatctc acgatatttt
     2401 cacgctaccg ctgtgtcggg agcatcacaa cgagcttcat gcggataccg tggcatttga
     2461 agagaagtat ggctctcagc tggagctgat atttcgtttt atcgatcgtg cgctggcaat
     2521 tggcgtactg gcgtaagtgg agaacgagca tgaaccttga ag
//
LOCUS       DAZFJF010000290         2498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFJF010000290 DAZFJF010000000
VERSION     DAZFJF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.292"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..557)
                     /locus_tag="ACHKAU_005194"
     CDS             complement(<1..557)
                     /locus_tag="ACHKAU_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5534053.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
     gene            complement(559..837)
                     /locus_tag="ACHKAU_005195"
     CDS             complement(559..837)
                     /locus_tag="ACHKAU_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534054.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(1005..1160)
                     /gene="hokD"
                     /locus_tag="ACHKAU_005196"
     CDS             complement(1005..1160)
                     /gene="hokD"
                     /locus_tag="ACHKAU_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5534055.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1765..>2498
                     /locus_tag="ACHKAU_005197"
     CDS             1765..>2498
                     /locus_tag="ACHKAU_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5534056.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASLYPEAIKAESV
                     LRNILTEKYSNFSIVQFPELNHQETFS"
BASE COUNT          692 a          574 c          558 g          674 t
ORIGIN      
        1 cgacgcgata ctgcctcagg taatgcgtcc accacctgat tgcagactgc ccaccaggat
       61 aattcagcca gcgataattc ccgttcctgt gtgccattca ttgcgtggcg gatgacgtca
      121 atcatccatg ctgacaggtt ttgttgagca agttgctcaa gtgattcgga tgtctggtcg
      181 cgcagctggt tgtcgcagtg ccagcacaac accatcgcgc cggtaccgta acgatgtatg
      241 acggtttcgc tgtggtggta atcaccatga ggccactggc aggatttaac gtgacgtaat
      301 aaccagtcag acagtgcacc agcgccacca gcagcacgaa tcacacgctc attgctgaaa
      361 aatggcagta atgatttatc ttccgccagc ggctggcgaa cggcaggaac gaccccggac
      421 ggcagattac gcatgctttt cggttcagac tccaccagca ccctgccgcc atgaaatacc
      481 cgcatggatt cacggcctgg cttaacgata accagaccga gttccggtac cagaacaggt
      541 cgaagtaata cccgcacatt acctccagat ccgttgctgg aatgtgcggg acggacgcgg
      601 tggccgttcg gagtaaggga gcctgacgga gattatccag tgacggtagt cgaggctaag
      661 ggctttttta acctcgcatc cgcgcctgcg gtaacactga atgagccatt cggcctgttc
      721 ttcagtgcat gggggatgct ggtaccagtc agatttgaat gcgtgaaaac accgtccgcg
      781 cctgctggca aagacggcag aatcatcaga attgtataat ttggtatcgt gcgccatcgg
      841 ttgtctctgc tggcgcagca ggtgccagtt gttcaggctg gcgtgcgaat tgtaaaccag
      901 aatgccagga aaaaacaaaa cccgccgaag cgggttaagt gcgggtgcgt tgaggatgcc
      961 tgattcatca gaggtggcga gggatttctc ccccgcctgg tctcttactc ctcaggttcg
     1021 taagctgtga agacagcgac ctccgtctgg ccggttcgga ttcgtacctc gcagaggtct
     1081 ttcctcgtta ccagtgccgt cactatgacg gttaaacaga tgacgatcag ggcgattaac
     1141 atcgcctttt gctgcttcat agcctgcttc tccttgcctt tcggcacgta agaggctaac
     1201 ctagatttgc cgttcataga ttgagcctca gattaatgtt aagcgtcttg caggacgcgt
     1261 aatgttaact ggggcttttc tctatctgcc gttggtgttc atgcccgagg cagatagcct
     1321 caagcacccg cagctattct acttaacttc ccattacctc gccaatatga aatcggtcag
     1381 aaagacagac gtcccataag gacaacaacg gaaaaagaga ttttgcttga tgctatttac
     1441 ctctgcacca tgaagttgcg ccaaaatccc agtggtgata acaggaattt ggttgttggt
     1501 gattggggaa agaagtacgt tagactgtgg gttagttcat tgacatgata gttacctgcc
     1561 ttcaatttcc taagcaagcg atgtaaacga taatcaatat caaatgtatt gacaggtaaa
     1621 ataataacga tgtacctttg ctgataacat atatcagtct taaatcataa tggaggtaga
     1681 taaaataact aaacacacac aacaaatttt ttatcagtga catagcatat cttaagcatc
     1741 ctttaatgct catggggaga acgcatgact aaacatatgg acattcattg tcaaacacgc
     1801 atatttaact caattaacca tgacaccaaa tataaaatat gtatttttac tccaaaaaac
     1861 attaaaaaaa ataatgcaac tctttatata cttgatggga atagtgccaa caattacatt
     1921 tctgatattc tgcctgttat tgatgcacta cccaatccac cagtgttagt tacacttggt
     1981 tatgaatctt ggaataacct tagcattcat cgccgtgctt acgattatac tcctgatggt
     2041 gaaaatgcta ttgttgataa ctctaaacca gcatggatct actttactgg tggaggaagc
     2101 cagtctttcc gagaattatt actaactcag atcatgccat gggttagtac tattgctccg
     2161 aacagttcca gaatcggtat atgggggcac tcgctaggtg ctatttttgt gcttgattgc
     2221 ttaaaaaata attcatgctt taactattat tatatatctg caccatcact tctatggcag
     2281 aatgaaagaa tcatcaagat aattaaagat gatatatcag aatcaaagca cactaaaagt
     2341 atatgcttgc ttaacggaaa tcttagcctt gattactccg catctttata tcctgaagcc
     2401 atcaaagcgg aatctgtttt gagaaatatt ttgacagaaa aatacagtaa tttttctatt
     2461 gtacaatttc cagagctcaa tcatcaggag actttctc
//
LOCUS       DAZFJF010000291         2468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFJF010000291 DAZFJF010000000
VERSION     DAZFJF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.293"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2295
                     /locus_tag="ACHKAU_005198"
     CDS             <1..2295
                     /locus_tag="ACHKAU_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5534057.1"
                     /translation="MEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQMREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPG
                     QQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLY
                     QLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGH
                     AGIGGGINAAITYSETKSLPASGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESV
                     GIGAGVDASVMTLACRTWQECWVN"
     gene            2307..>2468
                     /locus_tag="ACHKAU_005199"
     CDS             2307..>2468
                     /locus_tag="ACHKAU_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFO5534058.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYRDIYK
                     ITSSDFQTAT"
BASE COUNT          610 a          614 c          768 g          476 t
ORIGIN      
        1 atggagtacg atgctgccgg acgtgtcatc agtctgacta acgagaacgg cagccacagc
       61 gacttcagct acgatgcgct ggaccggttg gtacagcagg gcggctttga cgggcggacg
      121 caacgttatc attatgacct gaccggaaaa ctcacgcaga gtgaagatga ggggcttgtc
      181 accctctggt actacgatga atcggaccgt atcactcacc gcacggtgaa cggcgaaccg
      241 gcagagcagt ggcagtatga tgaccacggc tggctgacag acatcagcca cctgagcgaa
      301 ggccaccgtg tcgctgtcca ctacggctac gacgataagg gccgcctgac cggcgaacgg
      361 cagacggtgg agaacccgga gacgggggaa ctgctgtggc agcatgagac aacacacgcc
      421 tacaacgaac aggggctggc aaaccgcgtc acgccggaca gcctgccgcc ggtggagtgg
      481 ctgacctacg gcagcggtta tctggcgggc atgaagcttg gcgacacgcc gctgctggag
      541 tacacgcgcg accgcatgca ccgggagacg gtgcgcagct ttggcagcat ggcaggcagt
      601 aatgccgcat ataaactgac cagcacatac acccccgcag gccagttaca gagccagcac
      661 ctgaacagcc tggtgtatga ccgtgactac gggtggaatg acaacggcga cctggtgcgc
      721 atcagcggcc cgcgacagat gcgggaatac ggctacagcg ccacgggcag gctggagagc
      781 gtgcgtaccc tcgcaccgga cctggacatc cgcatcccgt atgccaccga cccggcggga
      841 aaccgcctgc cggacccgga gctgcacccg gacagtaccc tcacggcgtg gccggataac
      901 cgcatcgcag aggatgcgca ctatgtttac cactatgatg aatacggcag actgacggag
      961 aagacggacc ttatcccggc gggtgttatc cggacggacg acgagcggac ccaccactac
     1021 cactacgaca gccagcaccg tctggtgttc tacacgcgga tacagcatgg cgagccactg
     1081 gtcgagagcc gctacctcta cgacccgctg ggacggcgaa tggcgaaacg ggtatggcgg
     1141 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggagat gacgtggtac
     1201 gggtgggacg gcgaccggct gacgacggta cagaccgaca ccacgcgtat ccagacggta
     1261 tatcagccgg ggagcttcgc gccgctcatc cgcatcgaaa cagacaacgg cgagcgggag
     1321 aaagcgcagc gccgcagcct ggcggagaag ctgcagcagg aagggagcga ggacgggcac
     1381 ggcgtggtat ttccggctga actggtgcgg ctgctggaca ggctggagga agaaatccgg
     1441 gcagaccgcg tgagcagtga aagccgggcg tggcttgcgc agtgcgggct gacggtggag
     1501 caactggcca gacaggtgga gccggaatac acaccggcgc gaaaagttca tttttaccac
     1561 tgcgaccacc ggggcctgcc gctggcgctc atcagcgaag acggcaatac ggcgtggcgc
     1621 ggggagtatg atgaatgggg caaccagctt aatgaagaga acccgtatta cctgcaccag
     1681 ccataccgtc tgccggggca gcagcatgat gaggaatcag ggctgtacta taaccggaac
     1741 cggtactatg acccgctaca ggggaggtat attacacaag accccattgg gctggcgggg
     1801 ggatggaacc tgtataatta cccactgaat ccgataataa ggatggatcc tttgggtttg
     1861 tataatttat atcaattatt atatgatgtt tggcatgatg attcatatgg aacatcatca
     1921 attgatatta ctggcagtgg agatctaata tcattaggtg gtcatgcagg acttggcgtt
     1981 gcgtttgcta aaaagaaagg tgaaatgtta tctgatattt gtatttatgc tacagcatgc
     2041 ggacatgcag gaattggtgg tgggataaat gcggctatca catattctga gaccaagtct
     2101 ttacctgcat cgggagtcag caattcagta ggtgtaacgg ttggcggcgg agttgggggg
     2161 cattttgcgt atacttatgt agtggatgtt gataatccag aatcatcgac agaatctgtt
     2221 ggtatcggtg caggtgttga cgcttcagtt atgactctgg cttgtagaac gtggcaagaa
     2281 tgctgggtca attaacagga ggaaatatga aaaaatatat atttttagtt ttatttttat
     2341 tgtgtggttg ttctccagtg gacatagagc cagctagaca agaagttaat atctttcgtg
     2401 gtttatatca gacagggaaa taccgtgata tatataaaat cacttcaagt gattttcaga
     2461 ctgccaca
//
LOCUS       DAZFJF010000292         2465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFJF010000292 DAZFJF010000000
VERSION     DAZFJF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.294"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1820
                     /locus_tag="ACHKAU_005200"
     CDS             <1..1820
                     /locus_tag="ACHKAU_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5534059.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHKAU_005201"
     CDS             1817..2023
                     /locus_tag="ACHKAU_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO5534060.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..>2465
                     /locus_tag="ACHKAU_005202"
     CDS             2020..>2465
                     /locus_tag="ACHKAU_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5534061.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNG"
BASE COUNT          597 a          620 c          732 g          516 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgctgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacacactt atctggttgc cgacggatgg tgatgccgag aactttatga
      241 aaacccacgt tgagccgacc atccgcgata ttccgtcgct gctggcgctg gctccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ctatgaagcg tttttccaat ggtcgtggct
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcggtg gatgtggcgg
      421 gttatgatga actggctgcc tttgatgagg atattgaaca ggaaggctct ccgacgttcc
      481 tgggcgataa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgccag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtatct taaatttggc gacaaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg acccctccag cgtgttttat ctctgcgagc
      721 ataatgcctg cgtcatccgc cagcaggagc tggactttac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgaaccgcc tgacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaga cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aagcgaaaat cggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagtattatt cagcgcccgt tcctgatcgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgatgaac
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ctggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaaacatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 tcggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatcttaccg
     1561 aagcgcagca actgactgct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaag cgacgcaatg aggcgctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcactg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagaatac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggaaaac gggtggcaac ggtacagaaa gacggacggc gagtggagtt
     1921 tacgaccact tccgtgtctg acctgaaaaa atacattgct gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gaccagcagg attttatgta tgaaaatgtc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgtgaat atgccggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgggtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccga
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagag tttgccgaag atgactgttg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacgg
//
LOCUS       DAZFJF010000293         2465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFJF010000293 DAZFJF010000000
VERSION     DAZFJF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.295"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1820
                     /locus_tag="ACHKAU_005203"
     CDS             <1..1820
                     /locus_tag="ACHKAU_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5534062.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHKAU_005204"
     CDS             1817..2023
                     /locus_tag="ACHKAU_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO5534063.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..>2465
                     /locus_tag="ACHKAU_005205"
     CDS             2020..>2465
                     /locus_tag="ACHKAU_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5534064.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNG"
BASE COUNT          596 a          624 c          733 g          512 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgttgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacaccctt atctggttgc cgacggatgg cgatgccgag aactttatga
      241 aaacccacgt cgagccgacc atccgtgata ttccatcgct gctggcgctg gccccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ccatgaagcg tttcaccaat gggcgtggtt
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg
      421 gttatgatga acttgctgcc tttgatgatg atattgaaca ggaaggctct ccgacgttcc
      481 tgggtgacaa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgtcag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtacct taaatttggc gataaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgaac
      721 ataatgcctg cgtcatccgc cagcaggagc tggacttcac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgagccgcc ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgacgagc
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ctggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 ttggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatctgaccg
     1561 aagcgcagca gctgacagct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaaa cgacgcaatg aggctctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcgctg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggtaaac gggtggcaac agtacagaaa gacggacgaa gggtggagtt
     1921 tacggccact tccgtgtctg acctgaaaaa atatattgca gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgcc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgcgaat atgctggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgagtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccgg
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagaa tttgccgagg atgactgctg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacgg
//
LOCUS       DAZFJF010000294         2457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFJF010000294 DAZFJF010000000
VERSION     DAZFJF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.296"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            222..1169
                     /gene="stxA1a"
                     /locus_tag="ACHKAU_005206"
     CDS             222..1169
                     /gene="stxA1a"
                     /locus_tag="ACHKAU_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO5534065.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1179..1448
                     /gene="stxB1a"
                     /locus_tag="ACHKAU_005207"
     CDS             1179..1448
                     /gene="stxB1a"
                     /locus_tag="ACHKAU_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5534066.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            1948..>2457
                     /locus_tag="ACHKAU_005208"
     CDS             1948..>2457
                     /locus_tag="ACHKAU_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5534067.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
BASE COUNT          639 a          482 c          636 g          700 t
ORIGIN      
        1 gtaacatcct gtgtatcaat aaatgttgtt atctacgtac gtcaagtagt cgcatgagat
       61 ctgaccagat atgttaaggt tgcagctctc tttgaatatg attatcattt tcattacgtt
      121 attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt cagggcgtgg aggatgtcaa
      181 gaatatagtt atcgtatggt gctcaaggag tattgtgtaa tatgaaaata attattttta
      241 gagtgctaac ttttttcttt gttatctttt cagttaatgt ggtggcgaag gaatttacct
      301 tagacttctc gactgcaaag acgtatgtag attcgctgaa tgtcattcgc tctgcaatag
      361 gtactccatt acagactatt tcatcaggag gtacgtcttt actgatgatt gatagtggca
      421 caggggataa tttgtttgca gttgatgtca gagggataga tccagaggaa gggcggttta
      481 ataatctacg gcttattgtt gaacgaaata atttatatgt gacaggattt gttaacagga
      541 caaataatgt tttttatcgc tttgctgatt tttcacatgt tacctttcca ggtacaacag
      601 cggttacatt gtctggtgac agtagctata ccacgttaca gcgtgttgca gggatcagtc
      661 gtacggggat gcagataaat cgccattcgt tgactacttc ttatctggat ttaatgtcgc
      721 atagtggaac ctcactgacg cagtctgtgg caagagcgat gttacggttt gttactgtga
      781 cagctgaagc tttacgtttt cggcaaatac agaggggatt tcgtacaaca ctggatgatc
      841 tcagtgggcg ttcttatgta atgactgctg aagatgttga tcttacattg aactggggaa
      901 ggttgagtag cgtcctgcct gactatcatg gacaagactc tgttcgtgta ggaagaattt
      961 cttttggaag cattaatgca attctgggaa gcgtggcatt aatactgaat tgtcatcatc
     1021 atgcatcgcg agttgccaga atggcatctg atgagtttcc ttctatgtgt ccggcagatg
     1081 gaagagtccg tgggattacg cacaataaaa tattgtggga ttcatccact ctgggggcaa
     1141 ttctgatgcg cagaactatt agcagttgag ggggtaaaat gaaaaaaaca ttattaatag
     1201 ctgcatcgct ttcatttttt tcagcaagtg cgctggcgac gcctgattgt gtaactggaa
     1261 aggtggagta tacaaaatat aatgatgacg atacctttac agttaaagtg ggtgataaag
     1321 aattatttac caacagatgg aatcttcagt ctcttcttct cagtgcgcaa attacgggga
     1381 tgactgtaac cattaaaact aatgcctgtc ataatggagg gggattcagc gaagttattt
     1441 ttcgttgact cagaatagct cagtgaaaat agcaggcgga gattcataaa tgttaaatac
     1501 atctcaattc agtcagttgt tgccggtctg ataatagatg tgttagaaaa tttctgcatg
     1561 gtgaatcccc ctgtgcggag gggcgactgg tgaacggtat gatctctttg atgatcgtaa
     1621 gcgagaatac gcgggtttgg tggcaccagg ccgaactcac cgggaggcac ccggcatcat
     1681 gctgtataca gagattaggc atatatccag gctcctcatc gcaggagcct ttttacatgc
     1741 aaaaaaaagc ccgagtgggt tcgggcaaca gcatgagata cttgcattgt catttttatc
     1801 gtgcggattt taaccaggat tcataaggct gcgcaactgc gcggcctttt tcgtatttcg
     1861 ggctgtagtc cccgtgtgtc attcaggctt ccggactaca gcccactcca tatctgattt
     1921 aatacactat cccggccggg aggaataatg acatttaaac attatgatgt tgtcagggcg
     1981 gcgtcgccgt cagaccttgc ggaaaagctg acacacaaac tgaaagaggg ctggcagccg
     2041 tttggtagtc cggtggccat aaccccttat accctgatgc aggccattgc ggcggaaggt
     2101 gatgtcacca caccagtggc ggtgaccggt aatgagggta aggcggtggc tgtcagtgcc
     2161 accagcgacc cggagtatta ctttgttgtg gttctggcag ggcagtcaaa tggcatgtcg
     2221 tatggtgaag gtcttccgct gccggagaca tatgaccgtc cggacccgcg tattaagcag
     2281 ctggcgcgcc gcagtacggt gacaccgggc ggtgcagcat gcaaatataa cgacatcatt
     2341 ccggcggacc attgtctgca tgatgtgcag gacatgagcc gtcttaacca tccgaaagcg
     2401 gacctgtcaa aagggcagta cggtaccgtg gggcaggggc tgcatatcgc caaaaaa
//
LOCUS       DAZFJF010000295         2438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFJF010000295 DAZFJF010000000
VERSION     DAZFJF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.297"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..732)
                     /gene="cif"
                     /locus_tag="ACHKAU_005209"
     CDS             complement(<1..732)
                     /gene="cif"
                     /locus_tag="ACHKAU_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFO5534068.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKM"
     gene            complement(956..1837)
                     /gene="nleH"
                     /locus_tag="ACHKAU_005210"
     CDS             complement(956..1837)
                     /gene="nleH"
                     /locus_tag="ACHKAU_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFO5534069.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRNEFNPIDISSYNISERSWSENQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(1943..2212)
                     /locus_tag="ACHKAU_005211"
     CDS             complement(1943..2212)
                     /locus_tag="ACHKAU_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5534070.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(2214..>2438)
                     /locus_tag="ACHKAU_005212"
     CDS             complement(2214..>2438)
                     /locus_tag="ACHKAU_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5534071.1"
                     /translation="RFRLGPGNIIETNSNGWFPDTDGALITGLTFLDPKDATRVQGFF
                     QHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          664 a          614 c          442 g          718 t
ORIGIN      
        1 cattttaaca tttccatcaa tatcaaataa cgttgcaatc tgctccaaat ctgtcttatt
       61 ttgagacatg caggagaagt aattacaaat atcatccagc aaaatcggag tcaatgcttt
      121 atgcgtcatc cagtcctcaa atcttaactt tctggtgacg ccctctccta aatctgactg
      181 atacaagaat gcaggacgac aagagttttc tcctttggga atatcgacaa tgtaagcatg
      241 accaagcgca ccatcattaa cacgtaatac aaagctctta cattcaggca tacaggacag
      301 gcgctcctga agctgctcga gcgatagtgt ctgttcattt tcaaagcagt aactgaagcc
      361 atccttgtcc agaaatagtt tcataatagc attagcggta actccgcagg agggttccac
      421 aatgcctttc tctcgcccta taataacctc aacctcctca atcgacatcc cccagtctgt
      481 tgccatctgt ttgatgttag agtcgatatt ttgcattatt gcagcaggat ttttctgatg
      541 tataggtact tcgtggtcat cactatttag caatacatta gctttgaggt tctgtgagca
      601 cagggaagca aaatctctta catgtaaggc tgaagtgtgt tgcatgggag ataatacagc
      661 ttcagtattt acagatatgg cccctgtcag acaggacgcg gacgtcgggg ggggaagggt
      721 aatgtctttc atatctcatt cattcactct atatttatct ataaatacag gcgaggtgca
      781 aaaagaactt ctggcatggg cattatgtca tgtctgaaat ttttctggtg cctgtatgcc
      841 attcgccagt ctctcagcgg cattgtccat ttctgtgacg cggcctggat tgccagccac
      901 accacttttt accacctcca tccatgcaac aaatccacta cactggataa aattactaaa
      961 ttttacttaa taccacacta ataagatctt gctttcctcc atgataagat tgcattattt
     1021 gattttcact ccatgaacgt tcagaaatat tataagatga tatatctatt ggattaaatt
     1081 catttctcgt gcgatcatat aagacatttg tctctgttgt atcgataaaa agaattcctt
     1141 tttgctcaag cctgtcaaac atatcataaa tggcatgctc agcctgtgcc ggcaaggacg
     1201 aaatatttaa aagcgattct ccatttattt tatccattct aataccaata atatcgccat
     1261 tatcgccata tattttttct gcactcccgg caccataata ttggttgaag caacgaactt
     1321 cgtttgttac ctcttcattg ctttgagatg tagtaaacat cttcaggact tttgttgcat
     1381 cctcagcatc ttcatatact acagcgttac cccctttgcc aataacatta ccaggcacag
     1441 gcaagctgtt atagtccaca ctcggtaact ctggcggtgc ataatcaaca ggaggtaaat
     1501 caggtctatt cgaatgaaca gcacctcttt ccatagccct attcactggc gatgaattca
     1561 gcatcacctc aattttcctg ctaatctctc ccttaggcca tcccagcctg tgcaacagat
     1621 tagtaaagca accactatga ctttctcttg taacgcaaaa cttattatca gtgacaacaa
     1681 cacgatatgt tctgttgccc acctttactt gcgccccatt atccgagcta gcagctgcat
     1741 cccttacaga ggataaaata cgactgtcag gagaagtcag gtttctggtt aaagaattcc
     1801 atgaacaccc caaatttaca gaagatggtg atagcataca tttcaacctt caaaatgaat
     1861 caatctttac tttcttaaca aacatcacca tgacatgaca acaaaaaccg gagccggact
     1921 ccggttttgt gaagctgtcg ggttacttca tcccgccaat attttcccac gtcccgtcag
     1981 cacgcaggat ttgcagcggt cttaccacac actgtatcag ctttttatct gtatccagta
     2041 tcaccacctg tgtgattacc ctgtcctgct ccggaataat gccattctca tctgactcca
     2101 ggatgtctgc cggccccagt cgcagttgtg ctgtaagcga ctgcacgtgt tcacggccat
     2161 catgctttcc gcaaccacac agacgctgca taagtttttt tagtatattc atgtcattct
     2221 cctgttctgc ctgtatcact gcccacttca tccagcccct tgacatcctg ccacggcccg
     2281 tcaccaaacc tgacctgcaa atgctgaaaa aaaccctgaa cccgtgtggc atctttgggg
     2341 tcaagaaagg tcagtccggt gatgagtgcg ccatctgtat ccgggaacca gccattgctg
     2401 tttgtctcaa taatgtttcc cggccccaga cggaaccg
//
LOCUS       DAZFJF010000296         2437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFJF010000296 DAZFJF010000000
VERSION     DAZFJF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.298"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..458
                     /locus_tag="ACHKAU_005213"
     CDS             <1..458
                     /locus_tag="ACHKAU_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO5534072.1"
                     /translation="YGDDFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPL
                     QTLKKLKDAGYQTEVMIKTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLL
                     AENARKVFASNLSDKFAVYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(494..1285)
                     /locus_tag="ACHKAU_005214"
     CDS             complement(494..1285)
                     /locus_tag="ACHKAU_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO5534073.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(1291..2418)
                     /locus_tag="ACHKAU_005215"
     CDS             complement(1291..2418)
                     /locus_tag="ACHKAU_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO5534074.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT          624 a          681 c          524 g          608 t
ORIGIN      
        1 attatgggga tgattttgtt acccatacag cgaaattttc cggcgaaacg gtcgaacgtg
       61 caattgagag agcgatagtc agtaaactca acattgttgt cgaaggaaca tttcgtaatg
      121 ccgcgacgcc gttacaaaca ttaaaaaaac tcaaagatgc aggctaccaa acagaagtca
      181 tgataaaaac aacctcagcg gcattaagct gggaaagcac caatgaacgg tatagtaagg
      241 acaaagaggc gggaaatatt gcccgaaaag tggataaaaa ccaccatgat atcgtcaccg
      301 gtttgctggc agaaaatgcc cgcaaggtgt ttgcttcgaa cctgtcagat aagtttgcgg
      361 tgtattcgcg tgagaagatg atatttagtt cacaagcggc aacgaatgac gatattgcaa
      421 cgcttataca gaatgaaatc agcgggaata cacaataata ataagagctg atggcgtgaa
      481 ataacgccgg gctttatgcc cggcgcatta ccgtctggta cattaagcta tccagttcct
      541 taacgtcccc ttctctgcta tccggtaaca gcggtggaat ttccccacgc gccagttccg
      601 cacgaataaa cgcatgtaac aatggacggc gcggaccata ttccgcttct cctgcgcgct
      661 gtttacgttc cagtaattcg tctatttctg cacgtaacgg cgcatcaagt tcgcttcccg
      721 ccagtaactc agcaaagcgc attggcggta cgcctttacc cgcttcgacc cagcgcacgg
      781 ccagcagcgg acgcagcacg taaaagtatt ttttcagtcg aacttcgtcg ccctgcaaat
      841 atccggggaa gtttttctgc gccatcgaat agtaatgcca gcgcgcacgc agcggagaaa
      901 accacattgg gaccattgcc ttaaacgccg ttatcgtttc ttcatcttgt tgataaacca
      961 ccggagaatc cagccattct atcagcgtcg ggttcgcgcc tttcaataat cccagcgcct
     1021 tacgccactc ccaaccggaa acatctaact cgtcgtcgat gggcagctca ataacatcgc
     1081 gcggtgactc tactcgcaga taccattcca gcgggtgaac atacaaaaac cgtacatcgt
     1141 agtcgctgtc gggcgaggca aatccccagc cgcggctccc tgattcacag gcgtacagta
     1201 ctttcacatc ataccgccgt tcaatttctt ttaactgctg cgataccctt tctcgcatcg
     1261 cagcactgac tccatttaat ggcatgttac tcaccctttg atattaacca acccatgcag
     1321 aacctgatcc agaccgccaa atacagagat gttatcgatc cgttcagcca ctctctccag
     1381 cgtttccagt tcttttaagc gtaacgctac cgggttgttt tccatgactt tagcggtatt
     1441 gagtaacgaa cgcgtcgctg ccgtttcttc acgtcggcga atcacattgg cctgcgcgga
     1501 tttctccgcc tcaaccagct gcgcaaggat atttttcata tcgccaggca ggacgatatc
     1561 tttcacccct aacgaggcaa tctccatacc aaatggcaac atgcgcgatt tcacctgttc
     1621 gctgacaacg tcatcaatga cctgtttatc ttccagcaat tcatccaacg tacgcgtacc
     1681 taccgcttca cgcagggcaa attgtaattc ccgatacaaa tgatcaatcg gcttcgttaa
     1741 ctggctaaat gccaataaca cgtcgctgta gcgccagtta gcggcgaggt taatccgcag
     1801 attgacctta tctttcgtta agatttcttg cccgctgact tccagtacct gcaagcgggt
     1861 gtcaaccact tcagcgtcaa ccagatggtt aattttccag tatgcggtca gccccggcgg
     1921 taacagcgcc tgcgtttcac catcgatttt cagcacgcct acatgccagg ccggaacctg
     1981 caccgttagt attgcatcgc ggcctttaac tgcccctttg cgccgtggtt gcaataccgc
     2041 attcatcacc tccgtttgta cctggacttg ctgcgtattc atccgcacca gtgtcaaatc
     2101 atcctctaca cgccagtaca aacggcgcgt tgatggcggc aggatctcct gcaaaatacc
     2161 gtccatatac aacgccccgg cttcaatctc cgacaagtct gccaccaggc aatatttctc
     2221 cacccaatca ggctgaaagc ggcgcaaata atccgccagc acgtcgggaa cttcgctgcc
     2281 atcgagcgta atcagcaaga cttctggcgt atttaaccag ggtaaaagat gctcgcctgc
     2341 atgtagcact ttgtagtaat cgccgtttct ggagagtaat gccagctgat cttttcttac
     2401 gcttattttt ttaatcatta tctattcctt gcttaac
//
LOCUS       DAZFJF010000297         2436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFJF010000297 DAZFJF010000000
VERSION     DAZFJF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.299"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..122
                     /locus_tag="ACHKAU_005216"
     CDS             <1..122
                     /locus_tag="ACHKAU_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534075.1"
                     /translation="VKEYVEILKEFNICPEKVAEYLSEFSGKHFLDVYNYYSN"
     gene            304..489
                     /locus_tag="ACHKAU_005217"
     CDS             304..489
                     /locus_tag="ACHKAU_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO5534076.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            730..1719
                     /gene="nleB"
                     /locus_tag="ACHKAU_005218"
     CDS             730..1719
                     /gene="nleB"
                     /locus_tag="ACHKAU_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO5534077.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVDFSMYDKKLSDIYLENIHKQESYPASERDNYLLGLLRE
                     ELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGTLFTETGKTGCHNISPCSGCI
                     YLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKS
                     KVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTSSSW"
     gene            1768..>2436
                     /gene="nleE"
                     /locus_tag="ACHKAU_005219"
     CDS             1768..>2436
                     /gene="nleE"
                     /locus_tag="ACHKAU_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO5534078.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKYINN
                     KLSKI"
BASE COUNT          856 a          382 c          465 g          733 t
ORIGIN      
        1 cagtaaagga atatgtagaa attttaaaag aattcaatat atgcccagaa aaggtagcag
       61 aatacctaag cgagttcagc ggaaaacatt tcctcgatgt atataattat tattccaatt
      121 gaaaaaaata taaagtatct atcacggcac tctcagttga gtatcacagg cacatgtatt
      181 ttagccccct gagtctacag acaaccaata ttacttaaat aagtgataat cttaatgctg
      241 ttttttagaa cagatgattg atatttttgg gccggagaaa cgcagacagg ctttgttgaa
      301 taaatcgagc ttttgctgag tttaaggatc agatcactca ttttcctgca aaagctcatt
      361 gggcgaaaca atctgactca gagaacccgg attaaacgtc tggcccgcag aaccatctat
      421 ttctcacgca ctgtagtacg taataaaaaa gtcatcggta cttttatcaa aaaacatata
      481 ttctattaat tattgccatc acctttttct tattgacaac attactctgc tgattgttaa
      541 aatctaacct gtattcaggg ttacgattct atacaataga acagggaggg gaaaggatgt
      601 catcgttaat gagacttttt gttaatgtca acaatgctat gaatttattt tagaaaaaca
      661 attaagctga cgtagtgttc cgtctaaatg acggggcgat attaacatga ttagaacaag
      721 aggaatttta tgttatcttc attaaatgtc cttcaatcca gcttcagagg aaagacagcc
      781 ttatcaaata gtacacttct ccagaaagtt tcttttgctg gaaaagaata tcctctggaa
      841 cctattgatg aaaaaacccc tattcttttt cagtggtttg aagcaaggcc agagcgatac
      901 gaaaaaggag aagtaccaat attgaatacc aaagaacatc cgtatttgag caatattata
      961 aatgctgcaa aaatagaaaa tgagcgtata atcggtgtgc tggtagatgg aaattttact
     1021 tatgaacaaa aaaaggaatt tctcagtctt gaaaatgaat atcaaaatat aaaaataatc
     1081 taccgagcag atgtggattt cagcatgtat gataaaaaac tatctgatat ttaccttgaa
     1141 aatatccata aacaagaatc ataccctgcc agtgagaggg ataattatct gttaggctta
     1201 ttaagagaag agttaaaaaa tatcccagaa ggtaaggact ctttgattga gtcatatgca
     1261 gaaaaaagag aacatacttg gtttgatttt ttcaggaatt tggccatgtt gaaggctgga
     1321 actttgttta cagagacggg aaaaactgga tgccataaca tatcgccctg tagcggatgt
     1381 atatatcttg atgccgacat gattattacc gataaattag gagtcctgta tgctcctgat
     1441 ggtatcgctg tgcatgtaga ttgtaatgat gagataaaaa gtcttgaaaa tggtgcgata
     1501 gttgtcaatc gtagtaatca tccagcatta cttgcaggcc tcgatattat gaagagtaaa
     1561 gttgacgctc atccatatta tgatggtcta ggaaagggta tcaagcggca ttttaactat
     1621 tcatcgttac acgattataa tgctttttgt gattttattg aatttaagca tgaaaatatt
     1681 ataccgaata ccagtatgta taccagcagt tcatggtaag tcttccggtt gtggttgaaa
     1741 aatatcgttc tttaggtata gattcagatg attaatcctg ttactaatac tcagggcgtg
     1801 tcccctataa atactaaata tgctgaacat gtggtgaaaa atatttaccc ggaaattaaa
     1861 catgattact ttaatgaatc acccaatata tatgataaga agtatatatc cggtataacc
     1921 agaggagtag ctgaactaaa acaggaagaa tttgttaacg agaaagccag acggttttct
     1981 tatatgaaga ctatgtattc tgtatgtcca gaagcgtttg aacctatttc cagaaatgaa
     2041 gccagtacac cggaaggaag ctggctaaca gttatatccg gaaaacgccc aatggggcag
     2101 ttttctgtag atagtttata caatcctgat ttacatgcat tatgtgagct tccggacatt
     2161 tgttgtaaga tcttccctaa agaaaataat gattttttat acatagttgt tgtgtacaga
     2221 aatgacagcc ctctaggaga acaacgggca aatagattta tagaattata taatataaaa
     2281 agagatatca tgcaggaatt aaattatgag ttaccagagt taaaggcagt aaaatctgaa
     2341 atgattatcg cacgtgaaat gggagaaatc tttagctaca tgcctgggga aatagacagt
     2401 tatatgaaat acataaataa taaactttct aaaatt
//
LOCUS       DAZFJF010000298         2430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFJF010000298 DAZFJF010000000
VERSION     DAZFJF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.300"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..231
                     /locus_tag="ACHKAU_005220"
     CDS             13..231
                     /locus_tag="ACHKAU_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO5534079.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            233..790
                     /locus_tag="ACHKAU_005221"
     CDS             233..790
                     /locus_tag="ACHKAU_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5534080.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1081..1236
                     /locus_tag="ACHKAU_005222"
     CDS             1081..1236
                     /locus_tag="ACHKAU_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5534081.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1356..1700
                     /locus_tag="ACHKAU_005223"
     CDS             1356..1700
                     /locus_tag="ACHKAU_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO5534082.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1822..2094
                     /locus_tag="ACHKAU_005224"
     CDS             1822..2094
                     /locus_tag="ACHKAU_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534083.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2096..>2430
                     /locus_tag="ACHKAU_005225"
     CDS             2096..>2430
                     /locus_tag="ACHKAU_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534084.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGA"
BASE COUNT          637 a          545 c          658 g          590 t
ORIGIN      
        1 gggcggtaag aaatgaaaca ttatttagaa aaaaattacc cacgaaagag cagaacaaca
       61 gagtttctgt ttttcattct gtttatagtg ttgatgatac cgatatcccc gctattactg
      121 gtctggataa ttggaaggac atttgaacca gttattgagc tatataccga tgtgacatgg
      181 gaatcattca gcgcactgca caataaaatt aatccgtata aggaaaactg atatgagcac
      241 tattaccaga gaacgcgcgg agattaaatc atacatcaca ggcttcctga gcgactcggc
      301 gcacgataac aagtcttcag acagcctgct ggctaatgtg tttcgtatcg cgctggcatc
      361 actggaagca gagccgatag caatggtagt gcctgatgaa atggatttgc ttacctgcca
      421 tctcgacggt gtaactaaaa catatgctga tggctggaac gcctgccgcg tcgccatgct
      481 tcaggccgga aactttcggg aaaataagaa ttcgtcaacc aacaattttc gggaaatctc
      541 ggaaacgtca accagatctc cgataactct ggatggctgg ataagctgta ctgagcgaat
      601 gcctgaaaag agccagaacg tgcttatttc gatgaatatc gatagcgagg ctgggccatt
      661 aatatattcc gcacgctatc tcggaggcac gttccggcgc ggaggtatag cagttagtcc
      721 gggtaatgat cttaggcaag caacccactg gatgtcgcta ccagaaccgc cgcaggaggt
      781 gaatcaatga cctggcctga agcattcaca acggtaggaa ttgcgatggc ggtggcgctg
      841 gtggtgtatt cgatttgccg ctggggataa aaacggtttg cgggaaaagg atagttaagt
      901 agaattgcag cgggtgcttg aggctatctg cctcgggcat gaacaccaac ggcagataga
      961 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggctcaa
     1021 tccatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct
     1081 atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg
     1141 acggcactgg taacgaggaa agacctctgc gaggtgcgaa tccgaaccgg tcagacggag
     1201 gtcgctgtct tcgtagacta cgaatctgag aagtaagaga ccaggcgggg gagtaatctc
     1261 ccgccacctc tgatgtgtca ggcatcctca acgcacccgc gctttaccat actgaaaatg
     1321 ctgtttgaat gttcatctct gaaagaggac tatgaatgaa aaaggtattg attgcagcac
     1381 ttatttccgg tgtgtctttt ggcgcttttg cacagcaggg tggtttccag gggccagaag
     1441 cagagcgttc aacagtagcg caggcaaaag aactgaagga tgatgcatgg gttatccttg
     1501 aagggagcat cgttaaaaaa gtgggtgatg aacgttatga gtttcgtgac aatagcggga
     1561 caattgtcac ggatattgat gacagcgtat gggccgggca gaatgtttct ccgaaagaca
     1621 aagtaagaat tgagggtgaa attgataaag acctgagcag tgttgaagtt gatgtaaagg
     1681 cactgaaatt attaaagtaa ccgcccctgc ttgttaagcc cgtcttactg acgggttttc
     1741 tgtttgtaca ttccggcgta ttgccttaca attcgcgcag tcagcctgaa caactgacac
     1801 ctgctgtcac cggagaatcc gatgacacaa cacataaaat cccacaattc tgaagccgac
     1861 ccggaaatta agcaggggag gcgttttcgt gcgcctcagt atggctggtt tcactatctg
     1921 ttctgtacga tcgatgaggc agatatgctt caagaggcgt atctgcgtcg cggtgtccgt
     1981 gtggagcgga gtctgaacgc tgatcgtctg acctggaccg tttctgtata tcttcctgtt
     2041 cgtgcacatc tgccacggac acatgcctgc taccgtcagc gcgtctggag gtaatgtgcg
     2101 ggtattactt cgacctgttc tggttccgga actcgggctg gtggtcctta agcccggtcg
     2161 tgaatcattg ccagtttttc atcgcggcag ggtgctggtg gagccggaac cgaaaaacat
     2221 gcgggcgctg ccatctggag cggttcctgc tgttcgccag ccgctggcgg aagataaatc
     2281 actgctgcca tttttcagcg atgagcgggt gattcgtgca gctggcggcg ctggtgcact
     2341 gtctgactgg ttattacgtc acgtgaaatc ctgccagtgg ccacacggcg attatcatca
     2401 cagcgaaacc gttattcaca gttacggtgc
//
LOCUS       DAZFJF010000299         2393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFJF010000299 DAZFJF010000000
VERSION     DAZFJF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.301"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1166
                     /gene="escC"
                     /locus_tag="ACHKAU_005226"
     CDS             <1..1166
                     /gene="escC"
                     /locus_tag="ACHKAU_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO5534085.1"
                     /translation="RDTISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLSESLDKE
                     AQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTGKGA
                     VERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQIEISVSIIDV
                     DANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRVNALQQNSKA
                     KILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDNSNS
                     LTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLSAGESLLLGG
                     FIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKSASSE"
     gene            1180..1635
                     /gene="cesD"
                     /locus_tag="ACHKAU_005227"
     CDS             1180..1635
                     /gene="cesD"
                     /locus_tag="ACHKAU_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO5534086.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(2021..>2393)
                     /gene="grlA"
                     /locus_tag="ACHKAU_005228"
     CDS             complement(2021..>2393)
                     /gene="grlA"
                     /locus_tag="ACHKAU_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO5534087.1"
                     /translation="SVKNYDGEPLYILVSLWCKLQEKWISRNDIAEAFGINLRRASFI
                     ITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPVSTGPKRKNYRVG
                     NGIVGQSNIWNEMIMRRKKEN"
BASE COUNT          783 a          469 c          446 g          695 t
ORIGIN      
        1 tgcgtgatac aatctcagta aataaaaact cctgtaacgt gagaaaaatc acaacattta
       61 actcgattga agttcgtggc gttcctgaat gcataaaata cattacatct ttatctgaaa
      121 gtcttgataa agaagcacag tctaaagcaa aaaataaaga tgtcgttaga gtatttaaat
      181 taaactatgc atcagcgaca gatataacgt ataaataccg cgaccaaaat gttgtcgtgc
      241 ctggcgtcgt ctccatcctg aagacgatgg caagtaatgg ctcacttccc agcacaggca
      301 aaggtgctgt agaaagaaac ggcaacctgt ttgataacag tgtaaagatt tccgcagatc
      361 cacgtcttaa tgcagttgtt gtaaaagatc gtgaaataac tatggacata tatcggcaat
      421 taatttctga gcttgatatt gagcaaaggc aaattgagat ttctgtttca attattgatg
      481 ttgatgcaaa tgatcttcag caactgggag taaactggtc cggtacatta aatgcagggc
      541 aaggttctat tgcctttaac agctcaacgg cgcaagctaa tctcagttct tctgtgataa
      601 gcaacgccaa taatttcatg atcagagtga atgcgttaca acagaactcg aaagcaaaaa
      661 tactctctca gccatcaatt ataaccttaa ataatatgca ggcgattctt gataaaaacg
      721 taacgtttta tacaaaagtt tccggagaaa aggtggcatc tctggaaagt attaccagcg
      781 gaacattact gagagtaaca ccacgtatac ttgatgacaa ctcgaatagt cttaccggaa
      841 aacgtcggga acgagttcgt ttgttacttg atattcagga tggcaatcaa tcgactaacc
      901 aaagtaatgc tcaggatgcc agcagcatct tacctgaagt acaaaattca gaaatgacaa
      961 cagaagcgac attaagcgct ggagaaagtc tgctgcttgg cggatttatc caggataaag
     1021 agagttcaag taaagatggt ataccgttgt taagtgatat tcctgttatt ggtaacttat
     1081 tctccagtac aacaaaacaa aaacacagcg ttgttcgact ctttttgatt aaagcgaccc
     1141 cgataaaatc agcatctagc gaataataaa tgaggcttta tgagcaggaa atttagctct
     1201 ctacaggata tctatgattt ctaccaggat ggtggtacgt tagcatcact gacaaatttg
     1261 acgcaacaag atctcaatga tcttcattct tatgcctata cagcatatca atctggtgat
     1321 gttataaccg caagaaatct atttcatctg ctaacatatc tggagcactg gaattatgac
     1381 tacaccctat ctctggggtt gtgtcatcag cgtttatcaa atcatgaaga tgcacaactg
     1441 tgtttcgctc gctgtgcaac tttagttatg caagatccca gggcatctta ttattctggg
     1501 attagctact tattggtcgg aaatcggaaa atggccaaaa aagcctttaa ggcttgttta
     1561 atgtggtgta atgaacaaga aaaatacgct acatataaag aaaatattaa aaaattgtta
     1621 ggtaatacag attaaaaact accatctgcc ataccaatcc ccaggctgtc tggggatttt
     1681 ctatcggaaa taataaagct gctttggtga agtgcgccca acgttgagca ccgaccgctg
     1741 gcagatttat ctacaggagt gggttactcc attaaccagg attttatgcg cctgaaccat
     1801 cttctgctgt actcaatgtc atttccgctt atagaataag cagtatatat cttaacttag
     1861 catcagaaat ctgactgttt caatcatcta tagcccagag tgtatttgtc aggaaatgac
     1921 tttcaaactt tggcagagaa aaaggcttat cctggaaaac aaagcccttg aatagagctt
     1981 tatttttatt cttctataaa atatactcaa aaaaatacgt ttaattctcc tttttccgcc
     2041 tcatgatcat ttcgttccag atattagact gccccacaat accattaccg actcggtagt
     2101 tttttctttt tggtccggtt gataccggac tttctatcgg aacagcctcg aggttaacat
     2161 cataaatgaa aatctcaagg cgcttataat gcaagttacc ataactaaca tatctgacac
     2221 gaaatgaaat tttttctttt cttctcgata tataagttat aataaatgat gctctcctca
     2281 ggtttatacc gaatgcttcg gcaatatcat tgcgagaaat ccatttctcc tgcaatttac
     2341 accaaagaga aaccaagata tacagaggct caccatcgta attctttact gag
//
LOCUS       DAZFJF010000300         2374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFJF010000300 DAZFJF010000000
VERSION     DAZFJF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.302"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(104..1117)
                     /gene="argF"
                     /locus_tag="ACHKAU_005229"
     CDS             complement(104..1117)
                     /gene="argF"
                     /locus_tag="ACHKAU_005229"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5534088.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
     gene            1279..1695
                     /gene="rraB"
                     /locus_tag="ACHKAU_005230"
     CDS             1279..1695
                     /gene="rraB"
                     /locus_tag="ACHKAU_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO5534089.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1741..2244)
                     /locus_tag="ACHKAU_005231"
     CDS             complement(1741..2244)
                     /locus_tag="ACHKAU_005231"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5534090.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          624 a          585 c          557 g          608 t
ORIGIN      
        1 cgtatagcaa tagaaaaatt tggctggaag aaaaaaacta tcactgcata aaataatgtg
       61 atgccgggat gtttttgtat ttcccggcat cttgatagag atattaatta tttaatttac
      121 tgagcgtcgc gaccatcacc gctttgatag tatgcatgcg gttttccgcc tgatcaaata
      181 caatgctggc ggcagattcg aagacctcat cagtcacttc cattccgcca tgtaggccaa
      241 actcttccgc cattttcttg ccaagcgtcg tttggtcgtc atgaaacgcg ggcaggcagt
      301 ggaggaattt gacctccggg ttaccggtca actgcatcat cttgctgttc acttgataat
      361 cacgcagcaa tgcaatgcgt tcagcccatt tctcttttgc ttcacccatc gacacccata
      421 catcggtata gataaagtca gcaccttcaa ctcctttagc tacatcttcc gtcagcgtaa
      481 tattcccgcc attttgctgt gccagagcgc ggcattccgt aaccagcgca gcttccggcc
      541 agcatgcctg tggcgcgacc agacgcaaat ccagaccggt aagcgccgca gcttcgagca
      601 tcgaattgcc catgttgtta cgtgcgtcac ctgcatagac cagcgtcatt tcgttgaatg
      661 ctttaccggg taaatgctcc tgcatggtga ggagatccgc cagcaactgc gtggggtgaa
      721 actcattggt caggccgttc cataccggca cgccagcgta ttccgccagt gtttcgacaa
      781 tctcctgacc atagccgcga tactgaatac cgtcatacat gcgacccagc acgcgggcag
      841 tgtctttaat cgactcttta tgaccaatct gactgccgct tgggccgaga taagtaacgt
      901 gagcaccctg gtcatatgcg gcaacttcga aagagcatcg ggtacgggtc gagtcttttt
      961 cgaagatgag cgcgatgttt ttaccagtga gtttggcttc ttctttgccg cttttcttat
     1021 cggctttcag cttcgcggct aactgcagca ggctgttgag ttcagctggc gtgaaatcga
     1081 gtaatttcag gaaatgctta tgataaaacc cggacataga tccctcctgt ggctaacacc
     1141 tcaatgaatt aaaattcaat ttatatggat gattattcat ttgcaagtct aaagcataaa
     1201 tctttgtcac aaaggtggag gcaatgtcag tggtgtgtga caataagagt atcggcagga
     1261 cattaagagg aatgagccat ggcaaacccg gaacaactgg aagaacagcg tgaagaaaca
     1321 cgtttgatta ttgaagaatt actggaagat ggcagcgatc cggacgcgct gtataccatc
     1381 gaacaccatc tttccgcaga cgatctggaa accctggaaa aagcagcagt tgaagcgttt
     1441 aaactcggtt acgaagtgac cgatccagaa gagctggaag ttgaagatgg tgatatcgtg
     1501 atttgctgcg acatcctcag cgagtgcgcg ttgaatgccg atctgatcga tgcccaggtt
     1561 gaacaactga tgacgctggc ggagaaattt gacgttgagt acgacggttg gggcacttac
     1621 tttgaagatc ccaacggcga agacggcgac gatgaagatt ttgtcgatga agacgatgac
     1681 ggggttcgcc actaattaat tgattaaaat gcccgatagc acacactagc gggcattttt
     1741 ttagagttcg cgtagcatcc gcacttcaca atcgacatgg cccgtgcagc caagcgcata
     1801 gtcgatatgc tcaaagccca aatgctcata aagcgcaatg gcttccttta aaaaagcggt
     1861 cgtttccaga tagcagcgtt tgaagcccat ctctcgtgcc tgctccatcg ccattaaggc
     1921 cagttttttc gccagccctt taccgcgaat agccggaaga aaatacatct tttgcagttc
     1981 gcaaatgtcc gattcactac ctgctaatgg cgcaatccca ccgccgccga ccacttcacc
     2041 ctcgtactca accacccaat atgcatgacc cggctgacta tacacttgat acagctcgtc
     2101 aagattcgga tcggcgacgg tgtagccttt atcagcggta agaccgtatt cggcggatac
     2161 ctgacgaatg acgcgggcaa tagcaggatt atcctgcagc gtcaggcgac gcattacagg
     2221 tgattgcggc gcaatgttat tcatagttta acccaataaa aataaagtaa ttatacattt
     2281 gtttaatacc actctcttct tacgtgcgca agcatcttat aaagcaataa agtttttgcc
     2341 agcctattac tgataagagt attgtaacgt ggca
//
LOCUS       DAZFJF010000301         2324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFJF010000301 DAZFJF010000000
VERSION     DAZFJF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.303"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..810
                     /locus_tag="ACHKAU_005232"
     CDS             <1..810
                     /locus_tag="ACHKAU_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5534091.1"
                     /translation="DFLNGDPDQPIIMGRTYHHENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKPPDEKQDTPDMREY"
     gene            878..>2324
                     /gene="rhsD"
                     /locus_tag="ACHKAU_005233"
     CDS             878..>2324
                     /gene="rhsD"
                     /locus_tag="ACHKAU_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO5534092.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDT
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVV"
BASE COUNT          519 a          631 c          765 g          409 t
ORIGIN      
        1 gacttcctca acggcgaccc ggaccagccg attattatgg ggcgtaccta ccaccatgaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgacgatccg ctcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagccccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggaatt cagtaacaaa
      841 ccagcattaa aaataaacag aagaaggaat ttgaatcatg agcggaaaac cagcggcgcg
      901 tcagggagat atgactcagt atggcggtcc cattgtccag ggttcggcag gtgtaagaat
      961 tggcgcgccc accggcgtgg cgtgctcggt gtgtccgggc gggatgactt cgggcaaccc
     1021 ggtaaatccg ctgctggggg cgaaggtgct gcccggcgag acggaccttg cgctgcccgg
     1081 cccgctgccg ttcattctct cccgcaccta cagcagctac cggacgaaga cgcctgcacc
     1141 ggtgggcgtt ttcggccccg gctggaaagc gccttctgat acccgcttac agctacgtga
     1201 tgacggactg atactcaacg acaacggcgg gcggagcatt cactttgagc cgctgctgcc
     1261 gggggaggcg gtgtacagcc gcagtgagtc aatgtggctg gtgcgcggtg gtaaggcagc
     1321 acagccggac ggccatacgc tggcgcggct gtggggggcg ctgccgccgg atatccggtt
     1381 aagcccgcat ctttacctgg cgaccaacag cgcacagggg ccgtggtgga tactcggctg
     1441 gtcagagcgg gtgccgggtg ctgaggacgt gctgccagcg ccgctgccgc cgtaccggga
     1501 gctgacgggg ctggcggacc gcttcgggcg gacgctgacg taccggcgtg aggccgccgg
     1561 tgacctgacc ggggaaatca ccggcgtgac ggacggtgcc gggcgggagt tccgtctggt
     1621 gctgaccacg caggcgcagc gtgcggaaga ggcccgcacc tcttcgctat cttcttctga
     1681 cagttcccgc cctctctcag cctcagcgtt ccccgacaca ctgcccggta ccgaatacgg
     1741 ccccgacagg ggtatccgcc tttcggcggt gtggctgatg cacgacccgg catacccgga
     1801 gagcctgccc gctgcgccac tggtgcggta cacatatacg gaagccggtg aactgctggc
     1861 ggtatatgac cgcagcaata cgcaggtgcg cgctttcacg tatgacgcgc agcacccggg
     1921 ccggatggtg gcgcaccgtt atgcaggcag gccggagatg cgctaccgct acgacgatgc
     1981 ggggcgggtg gtggagcaac ttaacccggc aggcctgagt taccgctacc agtatgagca
     2041 ggatcgcatc accgtcacgg acagcctgaa ccggcgtgag gtgctgcata cagaaggcgg
     2101 ggccgggctg aagcgggtgg tgaaaaaaga actggcggac ggcagcgtca cgcacagcgg
     2161 gtatgacgcg gcaggaaggc tcacggcgca gacggacgcg gcgggacggc ggacagagta
     2221 cggtctgaat gtggtgtcag gcgatatcac ggacatcacc acgccggacg ggcgggagac
     2281 gaaattttac tataacgacg ggaaccagct gacggcggtg gtgt
//
LOCUS       DAZFJF010000302         2290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFJF010000302 DAZFJF010000000
VERSION     DAZFJF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.304"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(319..450)
                     /locus_tag="ACHKAU_005234"
                     /pseudo
     CDS             complement(319..450)
                     /locus_tag="ACHKAU_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            732..2090
                     /locus_tag="ACHKAU_005235"
     CDS             732..2090
                     /locus_tag="ACHKAU_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO5534093.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT          598 a          556 c          590 g          546 t
ORIGIN      
        1 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
       61 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
      121 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcattttgg tatcatgggc
      181 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
      241 ttaacccggt gtatcgttat gatcttatcc agcaattgaa ccgccccggg tttcctggag
      301 agtgttttat ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc
      361 tttttctgct tctgccggtg gaatatggcc ctgcctttcc agcaatcgtc gattgttata
      421 ccagtccacc cacgtgagtg tggtcagctc atcatactga cacgtgcatt acccctaccg
      481 cgataatgca accgtacaat atagggcgtt tcctaaacgc agccctcatc actccaatcc
      541 aacaagacac agcatttttg atcacgaaga aagacaaata atttccgtaa atgtttccgt
      601 tgttacaaat aacaacagtg ttttatattt ctttggcctc aggcccgcgc atgttgccat
      661 tcgatgtcat tttcctgact cgtttcttct ttatggtgta gtgtgatctt cactttatgg
      721 atgctaacaa tatgaaaaga aaaattattc ccgtgctaat cggttgtacg ctctctttct
      781 ctgccctggc ggcacaacct acggctgagc gctatgtcgt cagttttcca gaaggcacgc
      841 acgtgaacta tgccggcgca tttgccagtg cattcccgaa cggccttccg gttgggatag
      901 gttctggcct gctgttcacc ggtaaacagg gcgatgccct gacattcgca accattaccg
      961 atcgcggtcc gaacgcggat tcaccgaaag aggggaaaaa cgaaactaaa attttcgtta
     1021 cgccagattt cgcgccgctg ctgatgacga ttcgtgtaca aaacggcaaa gcggaggcca
     1081 tcgatccccg tcctctgcat gacgataaag gcgcgatcaa cggtctgccg ctggcaagcg
     1141 acgtgattgg ctccaccaat gaagtggcct tcagcgacac gttacataga ctgaaggggg
     1201 ataatcgcgg gctggatacg gaagggatca ctccggatgg taaaggaggt tactggctgt
     1261 gcgatgagta tggcccgttc ctgattaaca tcgacagtaa agggaaaatc ctggcaatcc
     1321 acggtccgca ggcggcggaa ggggaaaaag cgattgcggg cggtttgcca aatatcctca
     1381 aatggcgtca ggcgaaccgc ggctttgaag gcctcacccg tatgccggac gggcgtatta
     1441 ttgtcgccgt gcaaagtacg ctggatatcg acgctaagag caagaaaaag gcgctgttta
     1501 cgcgtctggt gagcttcgat ccggcaagcg gaaaaaccgc gatgtacggt tatcctatcg
     1561 acagcgcggc ctacagcaaa aacagcgacg ctaaaatcgg agacatcgtg gcgctcgata
     1621 accagcacat cctgctgatc gaacagggaa gagataaaaa caacagaatg cgtaacctga
     1681 tttacgaggt cgatctcaat aaggcgagcg acctgtcagg cttcgacaag ccgggtgagt
     1741 atccggagtt tgatgatgag aaaaccctgt cacagcgcgg cattaccctt gcgcagaaaa
     1801 cgcaggttgt tgatctgcgc tccttgggct ggcagcagga gaaggctgaa gggttggcgc
     1861 tgatcgacag caaaacgctg gcggtggcga acgataacga ctttggcgtg aaggtggcga
     1921 tgcaacatcc ggtcgagggc aagacattca aggattatcg ggtgaacgcg gaaggaaagc
     1981 tgacgcttga cgacaagcag gttgaaacga ccctcagagt gaagccactg gagaaaccgg
     2041 agtctgacag tgaactgtgg attgtgacgc tgccggaagc gttgaaataa gtttataagg
     2101 tacggacagc agtatcgctt agtttttcaa attcatgtat gtgaacaata ttggggttcc
     2161 ggcgtagacc cccaatgaaa gcgtcaccac gtgaaaacat gcctgcacgg tttacacgtc
     2221 atctcatggt ggcagccggt taactttcct gctacaccag tggaaaaagg ccataagcag
     2281 tccgaacaaa
//
LOCUS       DAZFJF010000303         2289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFJF010000303 DAZFJF010000000
VERSION     DAZFJF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.305"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(71..409)
                     /locus_tag="ACHKAU_005236"
     CDS             complement(71..409)
                     /locus_tag="ACHKAU_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5534094.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(412..717)
                     /locus_tag="ACHKAU_005237"
     CDS             complement(412..717)
                     /locus_tag="ACHKAU_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO5534095.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            complement(1032..1682)
                     /locus_tag="ACHKAU_005238"
     CDS             complement(1032..1682)
                     /locus_tag="ACHKAU_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5534096.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            1763..1948
                     /locus_tag="ACHKAU_005239"
     CDS             1763..1948
                     /locus_tag="ACHKAU_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5534097.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            2058..>2289
                     /locus_tag="ACHKAU_005240"
     CDS             2058..>2289
                     /locus_tag="ACHKAU_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5534098.1"
                     /translation="MAQASYSKLTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALD"
BASE COUNT          579 a          516 c          475 g          719 t
ORIGIN      
        1 cccatttcac tgcgtggctt gctgtaataa attaggttag ttcagccaat aaaaaaccca
       61 ccgaagtggg ctatgaccat tttttatttg gatttcgttg gtgagcgtga ttaacaactc
      121 tgtgcattac atcctcatat ttttcatctt caattttttc gacatcgcga ggaaatggtg
      181 ttgctaatgc tttgtcaact ttgtccattg ggtcttcatt aatcttatat tcaggaccgt
      241 catctatagc attaaatcca ggtgttacac cgttttttaa tgcatatgct atcctctttt
      301 cccatctcgc tattctcctc ctgtctcgag atgtaagacc tctatcagat acttttctgt
      361 tttgtccgcg gtcaggatta acataaatag tctttttcac cataagcata ctcaataagc
      421 accgtacggt agtttactgt acaattttat tttttggact gcatgtattt tgtttcctaa
      481 tgggtttgaa tccttgtaat aaatacttct attttttcga acaacttctt ctttcttctt
      541 gcagcaaagg cttcctagtg atgctgcttt gtctgctctg acgcaaccag agagctttag
      601 cgcaattttt cgcgccagtg cttcattact gcgtcgctcg gcaataagtt ctgctctgcg
      661 agctttgtag cggctttttg ccgtaccttt ggattctttc cagacaatgg ttaccatgat
      721 ggtctccttt aagtggcttt ggcgcatgac gcgtcgaggt gcttatcttc tcgatcgctg
      781 tcttgcagct gcaattcgcg ccatccccaa aaccactcaa gttctggtct caacggttag
      841 gttgagagtt cgtcgatgtt aaagagcctg ccaatctgtt ccgtttggct tccagcctcc
      901 tgctgacggc ttaaaattaa gacctcttaa ttttttggtc aagtgcattt ttgaagaaaa
      961 cttaatttta tgggcgcgaa tttagtttgt ctttgatttt taacgggaaa taaaaaaggg
     1021 gcgaaagccc cttaaggaag gtttgctagc ttggcatcaa cgacaacgcc aatgatttta
     1081 cagttcccat tgatttcaat cattgggtat tgtggattga gtggtttcag gaattttcta
     1141 ccggcatcaa taactaactt tttgaatgtc gcctcgtttt ctccttcaag tttggcgact
     1201 accagctttc cattacgtgg ttcgacttct gggtcgacga gaataatcat cccttcagga
     1261 atactcagtc ctgccggggc agtcattgaa tcacctttaa cgtcgagcca aaaagagtct
     1321 tcagaacaat ctaccgttgt gtcgtaccag ttatctattg cacgcctatg atatggctct
     1381 acagcttcca tccaacatcc tgcgcttacc caactaatta gaggatacga acctcttgga
     1441 tcatgcctgc tgtgataggc aatgtttgaa agactatcct ctcctttcaa caggtaatca
     1501 ggggagcact gcaaagcctt ggctaaggcc aataggtttt cgccattggg ctcagtttca
     1561 gatcgctccc attgggaaat agcaacatta gacacgccaa ccatcttgcc aagggcagcc
     1621 tgcctaatct tgagttcttt tctgcgagcg cgaatacgct cacccatcag ttgtgtattc
     1681 atagttaaga catcttaaat aaacttgact taagattcct ttggtggata atttaagtgt
     1741 tctttaattt cggagcgagt ctatgtacaa aaaagatgtt attgaccact tcggaaccca
     1801 gcgtgctgtt gctaaagcac taggcattag cgatgcagca gtctctcagt ggaaagaagt
     1861 tatcccagag aaagacgcct atcgattgga aatcgttaca gctggcgccc tgaagtatca
     1921 agaaagtgct taccgccaag cggcataagt aaattgctct ttaacagttc tggcctttca
     1981 cctctaaccg ggtgagcaaa catcagcggc aaatccactg ggtgtgccgc tatatatcaa
     2041 tataggaaaa ttaacaaatg gcacaagcaa gttacagcaa gctaacacag cgagaaattg
     2101 atcgcgcaga aacagattta ctcatcaacc tgtcaacgct tacccagcgc ggtctggcaa
     2161 agatgattgg ctgtcatgaa tcgaagataa gcagaacgga ctggagattt attgcttcgg
     2221 tcttgtgtgc tttcggaatg gcatcagaca tcagtccgat tagcagggct tttaagtatg
     2281 cgcttgatg
//
LOCUS       DAZFJF010000304         2280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFJF010000304 DAZFJF010000000
VERSION     DAZFJF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.306"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..776
                     /locus_tag="ACHKAU_005241"
     CDS             <1..776
                     /locus_tag="ACHKAU_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5534099.1"
                     /translation="SIIKGLDRLFEGINLDSAFSLSEEERSYFEEKAKSLNALAQLGI
                     SVEIISHELEEMDSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIRFLSPLKISGY
                     QSRQRITGKNIMDYVLKFFGERFERQRITIEFSEEFKQIAITDIPLRIYPVFTNIINN
                     AMYWVSLSNNRLIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGHGVGLYLC
                     RENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            778..>2280
                     /locus_tag="ACHKAU_005242"
     CDS             778..>2280
                     /locus_tag="ACHKAU_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO5534100.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLIIVDYHLDNNAPD
                     KTLKLLQDLKDSDHLNMIVIYTRENLETVWMQISSTLKGALDINSLIIDYDNEDVQSY
                     WEDVVLPNLNDNGNKALTRDEIIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMI
                     AEYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTL
                     NDSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEILKSDSPDIRC
                     RNIDFVFGNLSEELYQRLKNNNTLDEFIKSVFDSYSNEYANSGVAALLQYCSSKMDLP
                     SNNDTYHEMYHALNMNLSSKNFEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGND
                     PHHVRLSPHRLIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSIFEG"
BASE COUNT          775 a          350 c          429 g          726 t
ORIGIN      
        1 aatcaataat aaaaggtctc gatagattat ttgaaggtat aaacttagat tcagcattct
       61 cattatctga agaagaacgc tcatattttg aagaaaaagc taaaagttta aacgcgcttg
      121 cacagttagg tattagtgtt gagataatat ctcatgaact tgaagaaatg gattctatgg
      181 taaccagagg actaaactct cttcctactt ctgtaaaaga acaccctggt ttttcattgg
      241 cgttaaatgc tcacagatcg cttactcaac aaatacgttt cttatcacct ttgaaaatat
      301 caggttatca atccaggcag agaataactg gaaaaaatat catggattat gtcctgaagt
      361 tctttgggga gcgtttcgaa cggcaacgaa taactattga atttagtgaa gagtttaagc
      421 aaatcgcaat aacagatata ccattaagga tctatcctgt ttttactaat attatcaaca
      481 atgcaatgta ttgggtcagt ctgtcaaata ataggctcat aaagattggt tttgtgaatt
      541 ctttggttat catagcaaat tctggtccgg caattgatac cgatgatatc ccgcgactat
      601 ttgaactatt ttatagcaaa agagcaaatg gacatggggt aggtctgtat ctatgtcgag
      661 aaaaccttgc tgttgcacat cataaaatat ggtattcaga acctgatgaa ggcgataact
      721 atttaataaa agatggcgct aattttgtga tccagttcaa tggagtggag ttctaatatg
      781 acagtggcaa attataattc tcttgtccag aaaactttct gcgaaaatgc aattcgttcc
      841 gttgtcatga ttgatgacga ttttctgacg tattctgaat caatcagggc gttgaataac
      901 gaagttgatt tagactacaa caaaattgac tcatctaaac gagccgctac tcttgagagc
      961 ttttttcaat ctaaaaatat gatttgtgat gttgacaatg gttctgttaa tttcgatgtg
     1021 gatcggatta gaaaatcaga tcttattatt gtagattatc atcttgataa taatgcacct
     1081 gataaaacac ttaaactatt acaagatttg aaagactccg atcatttaaa tatgattgta
     1141 atatatacta gagagaattt agaaacggtt tggatgcaga tatcatcgac tctcaaaggt
     1201 gctctggata tcaacagctt gatcattgac tacgataatg aagatgtcca aagttattgg
     1261 gaagacgttg tattaccgaa cttaaatgat aatggtaata aagctctcac aagagatgaa
     1321 ataatagcct atattaaaga cagtaagcct tgtagaagaa ttaaaagatt aatacatgat
     1381 gatgctgtgt tggaggatca aaaggataaa aacttcattg caaaaatgat tgcagaatat
     1441 gctgtgtcta gaaatgcaat tatttctagc aacacatctg gcaatgtcat tcggggtgat
     1501 gaaagcggag taaaatggat tcaatgtggt aatatctttg tctccctatt tcataaggtt
     1561 caagatgatc atgaaaacga tggagatagg atttggcaaa ctctcaatga ttctctcatt
     1621 gaatggaaac catcttatta tcagttaata aaatctgaaa ttcagaatgc aatcgaagct
     1681 gaggctttat cttttgtaaa tcatttggct aacgatcatt acggtcaagc tgcgtggtta
     1741 aatgagatat taaaatcaga ctcgcctgat attagatgta gaaatattga ctttgtattt
     1801 ggtaatttat cagaagagct ttatcaaaga cttaaaaata ataatacgct ggatgaattt
     1861 atcaaaagtg tttttgatag ctattcaaat gaatacgcta acagcggagt tgctgcattg
     1921 ctccaatatt gctcttcaaa aatggatctg ccatcaaata atgatactta tcacgaaatg
     1981 tatcatgctt taaatatgaa tttgtcttca aagaattttg aagatggtca tatttctact
     2041 ggcactattt tctttgatac agagtcgaac aaatggtatt tatgtgtatc tgcggcatgt
     2101 gatttggttc ctactcaggg taacgaccct caccatgtaa gattaagtcc gcacaggctc
     2161 attaaagttc tggagctttt taacgccagt cagagtaaag cattgccatt tgctgaacat
     2221 tcgaaatata tatatgtaat gcataaaaat caaagaaaat atctctctat tttcgaaggg
//
LOCUS       DAZFJF010000305         2242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFJF010000305 DAZFJF010000000
VERSION     DAZFJF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.307"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..161)
                     /gene="ynfU"
                     /locus_tag="ACHKAU_005243"
     CDS             complement(<1..161)
                     /gene="ynfU"
                     /locus_tag="ACHKAU_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO5534101.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKA"
     gene            381..596
                     /gene="essD"
                     /locus_tag="ACHKAU_005244"
     CDS             381..596
                     /gene="essD"
                     /locus_tag="ACHKAU_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO5534102.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            601..912
                     /gene="ydfR"
                     /locus_tag="ACHKAU_005245"
     CDS             601..912
                     /gene="ydfR"
                     /locus_tag="ACHKAU_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO5534103.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            909..1442
                     /gene="rrrQ"
                     /locus_tag="ACHKAU_005246"
     CDS             909..1442
                     /gene="rrrQ"
                     /locus_tag="ACHKAU_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5534104.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1439..1936
                     /gene="rzpQ"
                     /locus_tag="ACHKAU_005247"
     CDS             1439..1936
                     /gene="rzpQ"
                     /locus_tag="ACHKAU_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO5534105.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          641 a          428 c          539 g          634 t
ORIGIN      
        1 gctttaagat ttgtctggaa tactttgttg caatgagggc agatcaaaag ggcacctttt
       61 tgtactcttg aaaaactgtg ttctgactct tgggtgcagt ttgggcagga acatttaacg
      121 agataattac ggcgtgattt tgagtcttta cgttctgaca taggcttttc ctgtataatg
      181 gccgtataca gtacactaaa tatgaaaaca tttctcgtat tattatttta tatatgactt
      241 tctttcaaaa taattactca catttttaat gtgtatgttt ctttagcgcc gttgagaaca
      301 acgtgtgctg tcaaaactac cccgtagact ccgatctttt tcaaacatat tgcaccatcc
      361 gtgtacatcg gggtgaggat atgaaatcaa tggataagtt aacaacaggt gttgcctatg
      421 gcacatcggc gggtaatgct ggtttctggg cattacagtt actcgataaa gtaactccgt
      481 cacagtgggc tgcaatcggt gtgctgggta gcctggtttt tggcctgctg acgtatctga
      541 caaatcttta tttcaagatt aaagaagaca ggcgtaaggc tgcgagagga gagtaatcca
      601 atgactcaag actatgaact ggttgtgaaa ggagtccgta attttgagaa taaagttacg
      661 gtaactgtag ccttacagga caaggaacgc tttgacggtg aaatttttga cctggatgtc
      721 gccatggacc gtgttgaagg agctgcgctg gagttttatg aggcagcagc cagaaggagc
      781 gtccggcaag tcttcctgga agtagcagaa aaattgtcag aaaaagttga gtcttatctg
      841 cagcatcagt actcctttaa gattgaaaat cctgccaata agcacgagcg tcctcatcat
      901 aaatatatat gaacacaaaa atcagatacg gcctgtcggc tgccgttctg gcgctgattg
      961 gtgctggcgc atctgctcct cagatacttg accagtttct ggacgaaaaa gaaggtaacc
     1021 acacaatggc ataccgcgat ggttctggca tatggaccat ctgtcggggt gccacagtgg
     1081 tggatggaaa aaccgttttt cccaatatga aactgtcgaa ggaaaaatgc gaccaggtca
     1141 acgccattga gcgtgataag gcgctggcat gggtggagcg caatattaaa gtaccactga
     1201 ccgaaccaca aaaagcgggt atcgcgtcat tttgtcccta taacattggc cccggtaagt
     1261 gtttcccgtc gacgttttat aagcggctga atgctggtga tcgtaaaggt gcatgcgaag
     1321 cgattcgctg gtggattaag gatggcggac gcgattgccg cattcgttca aataactgtt
     1381 acggtcaggt tattcgtcgt gaccaggaga gcgcattaac ctgctggggg atagaacagt
     1441 gaatcagata ttcatggtga tttttctcgt gttgtcagga tttatcgtcg gaaatgtctg
     1501 gagcgacaga ggatggcaaa aaaaatgggc ggaacgtgat gctgccgcat tatcacaaga
     1561 ggtaaatgct caatttgctg ctcgaataat tgaacagggg cgaactatag cccgtgatga
     1621 ggctgttaaa gatgcacaac agaaatctgc tgaaatttct gccagggctg cttatctgtc
     1681 tgatagtgtt aaccagttgc gtgccgaagc aaaaaaatat gccatacgcc ttgacgcagc
     1741 gaagcatacc gcagatcttg ccgctgccgt cagaggcaaa acaaccaaaa ccgccgaagg
     1801 aatgctcacc aacatgctcg gagatattgc agcagaagct cagctttatg ctgaaattgc
     1861 tgacgaacgc tacatcgcag gagtgacttg tcaacagatc tatgaatctt taagagataa
     1921 aaagcatcaa atgtagggta atattaaatc ggaacattta catcgcggaa tgtaaaattt
     1981 aaataaaaag gactcttcca tgagccaaaa ttcctgaaat cttaagggta agataaaagg
     2041 tcttaatcag aatgacacgt tttattaata aataaagcta ttctttcatt gctgtgtttt
     2101 tctttacaaa agtaatcctt gctatgggtg gttaatcatg cgttaatggt gttctggttt
     2161 gttacaaatt tatctgaagc agtcattgtt ataattttat tatttgtacc tcttgagatt
     2221 tccttgttgg tttttctctc tg
//
LOCUS       DAZFJF010000306         2224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFJF010000306 DAZFJF010000000
VERSION     DAZFJF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.308"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..546)
                     /locus_tag="ACHKAU_005248"
     CDS             complement(<1..546)
                     /locus_tag="ACHKAU_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5534106.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFTWQF
                     LEQYPAAQDCAYKFLVCEDKPGMPRPAIDSWDTEYMQENRWDEESASFIPVEPESDPI
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     KAMYPERKLFAIGWVKHKCKPG"
     gene            complement(640..831)
                     /locus_tag="ACHKAU_005249"
     CDS             complement(640..831)
                     /locus_tag="ACHKAU_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5534107.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(828..1016)
                     /gene="dicB"
                     /locus_tag="ACHKAU_005250"
     CDS             complement(828..1016)
                     /gene="dicB"
                     /locus_tag="ACHKAU_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5534108.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1584..1793
                     /locus_tag="ACHKAU_005251"
     CDS             1584..1793
                     /locus_tag="ACHKAU_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534109.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(1794..>2224)
                     /locus_tag="ACHKAU_005252"
     CDS             complement(1794..>2224)
                     /locus_tag="ACHKAU_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534110.1"
                     /translation="TAQNINTKPTEEEIDQFAHDAGLKNKKEKSPYAGNMFVYDNLIR
                     IRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAEKRWNVATWLFSN
                     RAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
BASE COUNT          590 a          516 c          478 g          640 t
ORIGIN      
        1 acccggctta catttgtgtt taacccatcc gatagcgaac agtttgcgtt ccggatacat
       61 agctttaatt tcaggcgttt tcatcagtgc ttcaacgata tgcccgtcaa aggtagccac
      121 gtcttcttgc agtaattcct gcgcgtcaat cgccatatca acggtgatgt tttcacatgt
      181 accgaactta accaggaccg cgttctgtac ttcaagggac agcttgtcaa aattgacgtt
      241 aataggatcg gattctggtt cgaccggaat aaaggaagcg gattcctcat cccagcggtt
      301 ttcctgcata tattcggtat cccaggagtc gatagcaggg cggggcatgc cgggtttatc
      361 ttcgcagaca agaaatttat aagcgcagtc ctgagcagca ggatattgct ccaggaattg
      421 ccaggtaaat ttggcacggg cgcggcgttc atcaccggct tcaatggcag tggctacagc
      481 aactgcgccc tcttctttta ttgcctgttc gtccggaatg gcggcgcaaa taaagacttt
      541 actcattttg ttttaacctc attacagatt taagggtgaa caaatccctg ccattgctgg
      601 catataaaaa tgaaaccgga tattaattac ggtgctgttt taaagtcctg ccggtatttc
      661 gttattatta gtgtgagtag ttttatctac cggataacag ttaccgggaa ttttttgttc
      721 tgctgctgca gccatgcatt ctttcattga accgtataag ccagtcacca gctcaagaga
      781 ttcgccggaa acaagataaa ctgtcagaac gagtgcaaat gttgtattca ttgtttatat
      841 cctttttgca gcaggtccag acgagccagc attgaaggaa tgcatacttc atttaacagg
      901 tcctgctcga gttttctctg cttaatggcg tcttcaataa atgttttgtc tccagtgata
      961 acgccaattt cgaaacgaag ttcagacgtg ctggcattac atgataactt ttccattatc
     1021 gcgtcctcaa caatgaattt tgtgatgcgg tgcctggtgc ctccaggtga cgttaaccag
     1081 ttaacaatta acgccggata cagagaatcc acccataaca ctgtttttgg ttttaactgt
     1141 tccgcgtgcg ctgagccgca ttcaccgcat cacaaaattc acttttaaaa aagggcggca
     1201 gagcagtcac ggagtaaaac tgataccgcc aaatgtcacc agaatattga taacagaggg
     1261 cgttgtagcg ggggtgtcac ttaagcgtat ggtcaacctg acaacccggt gtcctcaact
     1321 ggggaaggaa taaccccgcc atacttaccg ccgcgccatt tcgcggattg ccacaaccgg
     1381 aagcgcacgt tcgaagaaat ctaacgacaa gccttctaag ggaaagagct tcgccgtacg
     1441 ctttcgcgtt atgccctgac ttttcaggga aatatccttt cagtaaactg tcagtaccgg
     1501 attcttatcc gtgtccggcg cacgaccaca cgtgacagcg tgttggtctc catttttaac
     1561 ccagaacctc aatggaggat aaaatgccaa acaaaaaaag aaatccgctt attgaaaaac
     1621 agattgaatg cctggtaaat caactcaggc aatcagggtt attaaaaact cattcagagt
     1681 tgaggctcac agaatcagca ttcgacgata aattaaataa tgtcctttat aatggcatta
     1741 ttgattttaa tcgttctgtt ggtcgccgcg gccctgctgg tgtttcctta taattaccag
     1801 tcaatccaga gtggaccgtg ttcagcgtaa atataactgt acacatccag attatatttg
     1861 tggtctgtta agaacaggcc gcaaatacat gccgaagctt ccagtgcagc ggctctgtta
     1921 ctgaataacc atgtagcaac attccagcgt ttttctgcat cccagtcttt ctcaaggcct
     1981 gataccatga agaaaccgtt agtgttgcca tcaaataatt ctgtttccaa atttttaagc
     2041 aatgcctgat ggactcttgc caggtattcc gccggaattt cgccacgaat tctgatgaga
     2101 ttgtcataaa caaacatgtt ccccgcatat ggcgattttt ctttcttgtt ttttaaacca
     2161 gcatcatgag caaactgatc aatttcttct tccgttggtt tcgtattgat gttttgcgct
     2221 gtcg
//
LOCUS       DAZFJF010000307         2205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFJF010000307 DAZFJF010000000
VERSION     DAZFJF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.309"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..868
                     /locus_tag="ACHKAU_005253"
     CDS             134..868
                     /locus_tag="ACHKAU_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFO5534111.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNNADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACGSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQAAELKARGLLS"
     gene            865..1365
                     /locus_tag="ACHKAU_005254"
     CDS             865..1365
                     /locus_tag="ACHKAU_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5534112.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            1439..2011
                     /locus_tag="ACHKAU_005255"
     CDS             1439..2011
                     /locus_tag="ACHKAU_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO5534113.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
BASE COUNT          561 a          581 c          613 g          450 t
ORIGIN      
        1 cctgcgacct tttcctggct tgccggtctg aggatgagtc tcctgtgtca gggctggcac
       61 atctgcaatg cgtcgtgttg ttgtccggtg tacgtcacaa ttttcttaac ctgaagtgac
      121 gaggagccgg aaaatgtctg acaacaccat ccctgaatat ctgcaacccg cgctggcaca
      181 actggaaaag gccagagccg cccatcttga gaacgcccgc ctgatggatg agaccgtcac
      241 ggccattgaa cgggcagagc aggaaaaaaa tgcgctggcg caggccgacg gaaacaacgc
      301 tgacgactgg cgcacggcct ttcgtgcagc cggtggtgtc ctgagcgacg agctgaaaca
      361 gcgccacatt gagcgcgtgg cacgccggga gctggtacag gaatatgaca atctggccgt
      421 ggtgctgaat ttcgaacgtg aacgcctgaa aggggcgtgt ggcagcacgg ccaccgccta
      481 ccggaaggca catcatcacc ttctgagtct gtatgcagag catgagctgg aacacgccct
      541 gaatgaaacc tgtgaggcgc ttgtccgggc aatgcatctg agtatcctgg tacaggaaaa
      601 tccgctcgcc aacaccaccg gccatcaggg ctacgtcgca ccggaaaagg ctgtcatgca
      661 gcaggtgaaa tcatcgctgg aacagaaaat taaacagatg caaatcagcc tcaccggcga
      721 gccggttctc cggctgaccg gactgtcagc ggcaacactt ccgcacatgg attatgaggt
      781 ggcaggcaca ccggcacagc gcaaggtgtg gcaggacaaa atagaccagc aggcagcaga
      841 gcttaaggcc agagggctgc tgtcatgatt tactgcccgt cgtgtggaca tgttgctcac
      901 acccgtcgcg cacatttcat ggacgatggc accaagataa tgattgcaca gtgccggaat
      961 atttattgct ctgcgacatt tgaagcgagt gaaagctttt tctctgactg taaagattca
     1021 ggaatggaat acatttcagg caaacagaga taccgcgatt cactgacgtc agcctccggc
     1081 agtatgaaac ggccgaaaag aatgcttgtt accggatatt gttgtcggag atgtaaaggc
     1141 cttgcactgt caagaacatc gcggcgtctg tctcaggaag tcaccgagcg tttttatgtg
     1201 tgcacggatc cgggctgtgg tctggtgttt aaaacgcttc agaccatcaa ccgtttcatt
     1261 gtccgcccgg tcacgccgga cgaactggca gaaagcctgc atgaaaaaca ggaactgccg
     1321 ccagtacgct taaaaacaca atcatattcg ctgcgtctgg aatgagggct gccggttaac
     1381 accggccgtc gccgcacacc gtatttttat tcttcagcat gatgagaaag agataacgat
     1441 ggaaagcaca gccttacagc aggcctttga cacctgtcag aataacaaag cagcatggct
     1501 gcaacgcaaa aatgagctgg cagcggccga acaggaatat ctgcggcttc tgtcaggaga
     1561 aggcagaaac gtcagtcgcc tggacgaatt acgcaatatt atcgaagtca gaaaatggca
     1621 ggtgaatcag gccgctggtc gttatattcg ttcgcatgaa gccgttcagc acatcagcat
     1681 ccgcgaccgg ctgaatgatt ttatgcagca gcacggcaca gcactggcgg cggcactggc
     1741 accggagctg atgggctaca gtgagctgac ggccattgcc cgaaactgtg ccatacagcg
     1801 tgccacagat gccctgcgtg aagccctcct gtcctggctt gcgaaggggg aaaaaattaa
     1861 ttattccgca caggatagcg acattttaac gaccatcgga ttcaggcctg acgcggcttc
     1921 ggtggatgac agccgtgaaa aattcacccc tgcgcagaac atgatttttt cgcgtaaaag
     1981 tgcgcaactg gcatcacgtc agtctgtgta aaattccccg aaaatccgcc tgtttttact
     2041 gaaaaaagcc atgcatcgat aaggtgcatg gctttgcatg cgttttcctg cctcattttc
     2101 tgcagaccgc gccattcccg gcgcggcctg agcgtgtcag tgaaactgca ttaaaaccgc
     2161 cccgcaaagc gggcgggcga ggcggggaaa gcactgcgcg caagt
//
LOCUS       DAZFJF010000308         2204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFJF010000308 DAZFJF010000000
VERSION     DAZFJF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.310"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            351..1103
                     /locus_tag="ACHKAU_005256"
     CDS             351..1103
                     /locus_tag="ACHKAU_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO5534114.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRVEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            1100..1654
                     /locus_tag="ACHKAU_005257"
     CDS             1100..1654
                     /locus_tag="ACHKAU_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO5534115.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1656..1928
                     /locus_tag="ACHKAU_005258"
     CDS             1656..1928
                     /locus_tag="ACHKAU_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5534116.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          650 a          516 c          531 g          507 t
ORIGIN      
        1 gtgaatattg gtgaggagaa aagatccagt aataaactga aataaatatc gcgacttata
       61 tgaaaaagaa atatatgttt aattttatct ctgtgaacag tcatgaacag tagatgatca
      121 ctttaattaa aacactacac cacttaacca catgtattta ctatattttt attgaagtga
      181 acagtagtga atagtatgta ttaaaggaag cgtgtagatc agacatgaca cctttctctg
      241 gctagccaga acaaggtcat tgttcagtca ctgacacaat cctcctcggt agtgcgcttg
      301 tatggacccc ggcacaattg acacaacaac aaaacactac cggagcagtc atgactactg
      361 ttaaccagat ccctgatgca aacattacac cctccctccc accgaaaatt cgcgaagcgg
      421 tggataaagt taaggcagca aaagctgtct ggcaggaaga acgacgaaaa caaactgaag
      481 ctgctgcaat gattgaaact atccgtaaac gtcaggaaga tacaaaaacg gaaacgcagg
      541 cgcttaatga tgaatggcga aacctgtttc gtgagaatca gggaaatatg acgccacgca
      601 tgaaaaaact gcgggtagaa atcgccctcg gacgcgaaac actggatgag ttcgaagatt
      661 tgctggcagc tcaggcggca gaaaatgaat tcctgccctg gaaaactgcg gatgctgcaa
      721 accactacat cagcgaacat aatcgcctga ttgaaactca tgcagtgtgg ctctggaatg
      781 agtttatgaa ggaacacggt cagaaactta ttcagatcct tggactgctg aaaatgactc
      841 tgggccgaag cgcttctgct gttatcggtg tagttcatac cgtaaacgac cccgaaagtg
      901 tgctgaagca atttatcagc gagcaactca ccactccggc actgtcctgt aacgtatctt
      961 caaccgatga tattaccctg ccggggatca gcatttatgc ggacgataaa gccatccagg
     1021 atgccagaca atcccccagc cctgcagcgc gttcacggat gcttaaacag cgtgacatgg
     1081 ttaaaggggg cgagaagaaa tgaatgccgg aaccattact caggaagcac tcaacgatta
     1141 ccgctcggcg ataaaaagct ggctgacact acgtaatacg caaagtacct accagcttcg
     1201 tctggcggct ctgctggata ctgaagaaaa accggcagca tatgccagcc agcttgagga
     1261 tcttcgtgag cgtcttgcag tcctcgaatg gcagattaac tgtgctgcac gggatggtct
     1321 ttatgctcat cagattgtgc tggagagttg tgttacaagc gcaacggaaa acttcatgag
     1381 cgagcatggt gatgcactca ctgacgctct ggctcctttt ctttgcgctc catacgggct
     1441 tgaggcggca atgaaaatat tacgaaccgc tgtagcccga caaacggaaa tgcgtactcc
     1501 ggtaatttca gcagcatata agagcattat cgacgaaacc ggattaacgg tggatgcatc
     1561 aatgcgcgct gatgcttcag ccagtttcac cccggcacaa cataaagttt tcctggcccg
     1621 ccttaaccga cttaatgaaa aaggagtgtg ctgatatggc cctgaagtgt cctgaatgtg
     1681 gcgcggtcgc tcacgccaga accagcgcct atgaagctcc gtcggttaaa cgctcatggt
     1741 atcagtgcca gaatctggaa tgttcctgca catttaccgc actggaaagc gtggatacga
     1801 taattatgaa gcctcggtgc aatgaacagg aatcagacaa agcaaaaata ccggaaaaac
     1861 aacagcaaac tctcaatcgc tatggctccg cgtcaaagct gtcaaaccgt cagcaaattc
     1921 ctgtctgatt agcaaaatac gaccacgaag tcccggtcca gtaccgggat tttttacgcc
     1981 tttttcctgg ctggcctgag agcgtatgag tgcatatcta tggcgcatga aaacgcatga
     2041 gtctcatgcg ccatttttga cgcgaaagcc cttgtgtggt ggcttctgag acgatttacg
     2101 gggtgcatga aaaccagtct gttaagcgaa gcgggcaggc gggcggggca ttgcgcgcag
     2161 gaatgtgtat accgttttag taatgatcgc tgacatctca cttt
//
LOCUS       DAZFJF010000309         2191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFJF010000309 DAZFJF010000000
VERSION     DAZFJF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.311"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(113..802)
                     /locus_tag="ACHKAU_005259"
     CDS             complement(113..802)
                     /locus_tag="ACHKAU_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO5534117.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(817..939)
                     /locus_tag="ACHKAU_005260"
     CDS             complement(817..939)
                     /locus_tag="ACHKAU_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO5534118.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            1278..>2191
                     /locus_tag="ACHKAU_005261"
     CDS             1278..>2191
                     /locus_tag="ACHKAU_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO5534119.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTG"
BASE COUNT          694 a          481 c          404 g          612 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaacc cactcggcag
       61 cgggtttata cattttttac aacataccaa atttgcatga agtatatggc ttttaatcca
      121 gttttgcaat attttgctgt aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa
      181 gcaataaagc atcactatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
      241 taaatgtctc ggaccagttt tttgttgtca ctcccaccaa tgatgccagt gtctggtatt
      301 cataggcctc acgccctgca agttcgctct tcacatcctg tgcagccagc cagattaacg
      361 tcttcaggcg atccagtgtc ttacctgcaa tttttctgtt acttaacaaa tctttaaact
      421 cgctccatgc ccattgcgtt atggtgacct gatgccccca tcgaacgctt tcgctgtaac
      481 accaaagcaa ccatgctttc tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag
      541 aggttgagaa ttcaaccggg ctgaccaaag caatggatga accttttgcg tacgactgct
      601 taccggaagt cggcgtatta tccagcgtaa tcatcttgcc agttaccaca tccagaatgc
      661 gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctcctgc caggcttcga
      721 gctggccttt cgttgctccg ttcaagtcag cagtagctgc cataagttgc tcacgaacat
      781 actgtaaata ttgggtattc atgcagtaaa tcctttctat attttggcat aattcttcaa
      841 cattcggtaa tcgttcaaaa ccgaatcggg gaaacgacat aagcacagga gcccccagcg
      901 acagcgaagg agttctgata tataagactc agacatcatt cattccccgg ttctccaata
      961 tctgtttcac tcatcatcca taactacctg taattgcccc cccttttttt gtaacagttc
     1021 ttatattgct atatagaata gccattacta atgcttttaa atttaataaa ataaaaatta
     1081 taaaaaacat aaaacaccac gcaaacacac ttaataaaaa caccgttaca ttaaaagata
     1141 ataaaaaccg caataaaaaa cgaataaatc aattgtctca cgcaattata aaacatcata
     1201 ttgattacgc accttgtatt acaaactcat gtatgtaaaa tacgcgcacc attcaaaaaa
     1261 aaggaagaca ataacatatg aaaaaaagtg tcatcgctgg cgtctttatt gctctgtcat
     1321 ttaccacgtg ttcagctatc gcgaacagcc ttgcattatc attagcaaat gatgatgcag
     1381 ggaagtttca accaatactt aatgatattt atggcaataa acatgaaaac agagatgatt
     1441 actcacaagg cttatttctg ggatatagcc acgatatctc agactcgagc caattatctc
     1501 tccatattgc gcaagatatt tactctccat caggcagtaa taaaagacac aacacagctg
     1561 taactggaga cagagctttt agtgcataca ctcacactgg tattgaatgg aactcccttg
     1621 cgaatgactg gattcgctat cgattaggta ctgacatagg tgttgttggc cccgacgcag
     1681 gcggtcagaa agtacaaaat aaagctcatg agattattgg ggcagaaaaa tatcatgcat
     1741 gggatgatca aatagagaat cgctacggtt atactgtaaa agggatgcta tccatgacac
     1801 caagtatgga tattttaggt gctaatgttg gattataccc tgaagtttct gctgttactg
     1861 gaaacttatt tcaatatgta gcatatggcg caaccattgc cattggtaat gataaaacct
     1921 tcaattcgga taatggcttt ggtctgctgg ctccccgtgg tttaatgcat atgtccgata
     1981 caagcggatt caaatacaag atttttgcag gtatggaaag acgagatgtc aatcgcaact
     2041 atactctcga aggaaaaaca atacagacga aacaaacaac agtatcgcta aacaaaactg
     2101 ttgatgaata tcaagttggc gcaacaattg ggtatgcacc tgtagccttc acactagcat
     2161 ttaataaagt aacatcagaa ttcaagacag g
//
LOCUS       DAZFJF010000310         2174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFJF010000310 DAZFJF010000000
VERSION     DAZFJF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.312"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..356
                     /locus_tag="ACHKAU_005262"
     CDS             <1..356
                     /locus_tag="ACHKAU_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFO5534120.1"
                     /translation="IDIKFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKTLLN
                     GKYFDSGYIPCSRADESIQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPF
                     GQTILRKVTFSNIES"
     gene            393..2174
                     /locus_tag="ACHKAU_005263"
                     /pseudo
     CDS             393..2174
                     /locus_tag="ACHKAU_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          684 a          414 c          423 g          653 t
ORIGIN      
        1 tgattgacat taaatttata gctgatatac agtttaatac tgacttatgt ggtgacactt
       61 atatttccag aggtcccgtt ttatatgcaa ttgagcttga gagcgatatt ttaatcaaaa
      121 aaactctgct gaacgggaaa tattttgact caggatacat accctgtagt agagctgatg
      181 aatctatcca gttctcttat gctgatatgg aatctttttc atattcaacc tcaccagagg
      241 gcaaacaata cattgaaggc tgtttttatt taaacaagac taaaatagtc agaaaactta
      301 taccatttgg tcaaaccatt cttcgcaaag taactttctc caatatagag agttaatatt
      361 ttcgagtatt ataattaatt aaagggattt ctatgtacct tactgataat attatcaaac
      421 tgtcgaataa aataggtcag cgtgtttata ctgttgtctc tgaaatgtcg attgatgcat
      481 ggataacaaa agaaccgcag ccttttgttt atcgccgtag cggcgcttac cagaaactca
      541 ccattggctc agactggggt caactgtttg actgtgcctg gatgcgagtt tatggtaaac
      601 gtccagcaga taaatttaga cacaaactcg tcgctctcgt tgatatcggt ggcgaaggtc
      661 ttattgtgga taagaatggt tcacccatct gcggcatcac gaataaagca tcatcttatg
      721 gcgtaccgcc agataaacca ggtaaatggg tggttgacct ttctctcgtt agtgaaaatg
      781 acgaggtcga gttttggatt gatgctgcgt gcaacgattt atttggctat gtcactaatg
      841 gagggattat tactgatgtt catatcgcga catgtaatca actactgaaa tccctttatt
      901 atgatgttga agtgcttttc gactggatta atgatggtca gaagtttgaa tccatacatc
      961 ctaaaggtat tatcccagaa aaaattatca ccaaaagaag cgagcgtact gacgaaatca
     1021 taagaattct cgaatatatt gataatacgt taattacctt tagcaatgaa gaaataatta
     1081 aatgtcaaat tgcaatccaa tccataatta gtaaaaacaa taatccttca gagttcagga
     1141 ttatggctac tggtcatgcc catttagata tagcgtggat gtggccttta agagaaggac
     1201 gcagaaaagc aataagaaca tttgccaccg ccctgacaaa cattgaaaaa tatcctgatt
     1261 acatttttgg cgctagtcag taccaacttt ttcactggat aaaaaaagat tatccctatt
     1321 tcttcgaaaa gttaaaaaaa caaatcgccg caggccgctt tgagttacaa ggttgctttt
     1381 gggtcgaatg cgatttaaat ctggtaagtg gtgaatcgct gataaggcaa atcatgcatg
     1441 ggacacgatt cacactgcag aatttttcaa agaagataaa ttatgtctgg caaccggatg
     1501 tctttggatt ccccgccacg cttccacaaa ttctcaaaaa atcaggcatt aactatattg
     1561 catcgcaaaa gctctcacaa aacaagatta ataaatttaa taattacctt ttccgctggc
     1621 aaggcctgga tggctcagaa attctgatgc ataactttcc tgaagatacg tatgatagcc
     1681 gagcccgcgc tcgctcttta gaatatattg aacaaaacta taatgctgtc cctatgcgtt
     1741 tatggtgtat ggtgtcggtg atggcggcgc tggccccggt gaggaacata tagaacgatt
     1801 aacacgcata cgcaatattg atggcttacc tcatgttgat ttcagccgtg ttgataagtt
     1861 cttcactcac gccgacgcct ttcgcgaatc cttgcccatt atttcgggtg aactttactt
     1921 cgaggctcat caagggtgct ttacatcaga gtcagcaact aaagcccata atcgaattat
     1981 ggaaaataaa ctccatgatg ctgaattttt tataactatt actaataata tgacgtcaat
     2041 tcttcgcagt gagtttgatg aaatatggaa agcgatgctg accttgcaat tccatgacat
     2101 ccttcccggc tcatgtattt caagagttta tcatgaaact gaaaaggaat atcttaaact
     2161 agaagcaaaa accg
//
LOCUS       DAZFJF010000311         2144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFJF010000311 DAZFJF010000000
VERSION     DAZFJF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.313"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            272..1474
                     /locus_tag="ACHKAU_005264"
     CDS             272..1474
                     /locus_tag="ACHKAU_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5534121.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
     gene            complement(1661..>2144)
                     /locus_tag="ACHKAU_005265"
     CDS             complement(1661..>2144)
                     /locus_tag="ACHKAU_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534122.1"
                     /translation="SLDHEYPESCLQNDTNTYLLGRVIFLYNSMIYKFINCQEHETNN
                     IHSAMINNLLQEVDIALGKINDIIDSRNISAPHELANILTREKILTTREKKGNLISLF
                     DGFTLFHCVGMITFLIHYLRTPEEKVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE
                     "
BASE COUNT          590 a          412 c          516 g          626 t
ORIGIN      
        1 gccagttgtc gaaccccggt cggggcttct catccccccg gtgtgtgaaa tatacgaaaa
       61 aaaagcccgt actttcgtac gagctcttct ttaaatatgg cggtgagggg gaccgcctga
      121 aataaatctg acttaatcca ggggtgttat ataatttcat tataaacaat gtattacact
      181 ttttgttgtc cgatattatc taatgtattc ttagtgaagc gagagactaa tggcatactg
      241 gatggcatac cagagtgacg ggaattagcg tatggcagta ttgacggata cgaaagcaag
      301 acatatcaaa cctgatgaca aaccattgcc ccatggggga attaccggac tgacccttca
      361 tccttcttca gtaaaggggc gggggaaatg ggtttttcgt tatgtaagtc cggtgacaca
      421 aaaaagacgt aatgctggat tgggaactta cccagaggtc agtattgctg aagctgcacg
      481 tactgcccgg ataatgcgag agcaacttgc tgcaggtgat gatcctctgg agattaaaaa
      541 ggctgaatct gagaaagtcg ctatcccaac atttgccgat gcagccaggc gtgtacatgc
      601 agaactgtct cctggatggg aaaatccaaa gcatgtaagg cagtggttat cgacgcttga
      661 gaattacgcg tttcctcaac tgggagcaaa aacgctggat tcgattacgg ctgcggacgt
      721 ggcagaaaca ctgcgtccag tctggttaac cttgtcagaa acggcaagcc gggttaaaca
      781 gcgcattcat gttgttatgc agtggggatg ggcgcacggt ttttgtgtag caaatcctgt
      841 tgatgtggtt gaccatttgc ttcctcagca gacaagagga cgtgatgaac accaacccgc
      901 aatgccctgg aggcagttac cgctttttgt ggcgaccagt gtgtataccg atgaacctta
      961 taatgttacc cgcgcactgt tattaatggt gatacttaca gcaactcgct cgggcgaagc
     1021 caggggaatg cgctgggctg aaattgattt tcataagcgg gtatggacta tacctgcaga
     1081 aagaatgaaa gccaggatac agcatcgtgt tcctttatcc cggcaggcta tttacattct
     1141 ggaaaatata cgtggcctgc atgatgaact ggtgttccct tcacccagaa agcagcagat
     1201 cctttccgat atggtgttga caagttttct gcgtaaaaag aaagccgtca gtgacattcc
     1261 ggggcgagtt gccacggcac atggttttcg ctcaacattc agggactggt gtagcgaaca
     1321 ggggtattcg cgggatctgg cggaaagggc gctcgctcat acgctgaaaa ataaggttga
     1381 ggcggcatat catcgtactg atctactgga gcagcgtgta ccgatgatgc aggcatgggc
     1441 ggattatgtg atgtctcaaa ttgtgaataa ataatagtga aaatgtatat tgtacgatca
     1501 tgatttgata tgtgatcgta gcagtagtaa ttaagaaacc gcttttatgt caaatgtatt
     1561 gagctgataa ttatcagatc gtcaggaaag cgtattttga ttaacagggg attacttaac
     1621 aatccccttt ttataaaaaa taatttgcct gtttaactct tcactcctgc tgagactgaa
     1681 ttattcctag tgcgtcataa atcagtcttc tgcgtagttt attgttttta tctgcaccat
     1741 ataacataaa tatattttca actttttctt caggtgttct gagataatgg ataagaaagg
     1801 ttatcattcc aacacaatgg aataaagtga atccatcaaa caggcttatc aggtttcctt
     1861 ttttttcccg tgttgtaagt attttttcgc gggtaagaat atttgccagt tcatgggggg
     1921 ctgatatgtt tctgctgtct ataatgtcat ttattttacc aagggctata tcaacttcct
     1981 gtaataggtt atttatcata gctgagtgaa tgttattggt ttcatgttcc tgacagttta
     2041 tgaacttata aatcattgag ttatacagaa atattactct tcctaataaa tatgtgtttg
     2101 tgtcattttg aagacaggac tctgggtatt cgtgatccag tgaa
//
LOCUS       DAZFJF010000312         2144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFJF010000312 DAZFJF010000000
VERSION     DAZFJF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.314"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..608
                     /locus_tag="ACHKAU_005266"
     CDS             <1..608
                     /locus_tag="ACHKAU_005266"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5534123.1"
                     /translation="VVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYS
                     RAYLLRHPERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLP
                     PKAAGIAMALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADE
                     LHGDYINDKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(649..2079)
                     /locus_tag="ACHKAU_005267"
     CDS             complement(649..2079)
                     /locus_tag="ACHKAU_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5534124.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT          574 a          485 c          435 g          650 t
ORIGIN      
        1 ttgtcgtact gtgcgtgctg tttcgtaagc acattccgat gaataaatat ttcctcaagc
       61 aacagctggc ctgggtgcca tttcttggcc tggcgtgctg ggcgctggat atgccgttta
      121 tgaagcgtta ttcccgcgct tatttattac gtcatccgga gcgacgtggt aaagatgttg
      181 aaaccactcg gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt gtcaatttcg
      241 ttgaaggctc ccgtttcacg caagaaaaac atcagcaaac ccactccact tttcaaaact
      301 tgttgccacc aaaggcggca ggcattgcga tggcgctaaa tgtactgggt aaacaattcg
      361 ataaactgct taatgttacg ctgtgttatc cggacaataa ccgtcagccg ttcttcgata
      421 tgttaagcgg taaactgacg cgaattgtcg ttcatgtgga tttacagcct attgccgatg
      481 agttacatgg cgactacatc aatgacaaaa gctttaagcg ccattttcag caatggctga
      541 actcactgtg gcaggaaaaa gaccaacttc tgacgtcgtt gatgtcatca cagcggcagg
      601 acaagtaaat tttgctcgcc tctgaatgag cagaggcgag tgagtatttt aatgaacaaa
      661 acgtccggca cgagacataa attcttcttc gctcatctcc tgtccgttaa aaacaacttt
      721 acccggtgta taacgcagtt gcagcgaggc ggtattgttg tcaatcgtga ttaactgaaa
      781 catctgacct aaggtcatca tcccgctgat ttgtttatca gcccgctttt gcgctttttc
      841 cgcatccatt ccttcagata aattaagctg ttttgcggtt tctgtgacga cattaagcgg
      901 taacttcaca tcaaaattga gcgatttaat atctttgttt gtggatgatg aagatttggc
      961 tgggtcggca atactgatat ccagattggc atttagttcg ccgagtgcgt tcttccattt
     1021 taccggttgt ttaatggtcg gctcactttt ttgtaataac ggtaaatact ctttgaacaa
     1081 tgcagcattc acttcttgca gagcaacttc atcgtttgct aactcagggt gtgcagcaag
     1141 ctgcttctgc attgcaatgt tatactggat gataaattgg cgcaccgctg agggatcaat
     1201 agattcagca atcaatgacc actcaccact accgagttgc tgattccctt ttgttaactt
     1261 atcaagttcg taggtcagat tgacattgat ataatctttg tctttaacgc gatccagcat
     1321 ggtttttgca acgaaagcat cgatttgggc aacgtcttcc ccgtgattga taatgttaat
     1381 ctgattaaat ttcgactcgc tttcaccaac cggaaatttc ttttcttcta atcgcgccag
     1441 agaatcaatg gtaaaacttt ttgccgttac tttgttcatt ggtgataatt gcagaacaat
     1501 atcttcgact ttgccttcaa tatccaggtt agacaatgaa ctaccatcac cattaaaagt
     1561 aaactggccc tcaccaaaag aaaactttcc atactctgca ggggcaacat ctaatgtcgt
     1621 agagaatgag ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc
     1681 ggcgataaat agcggttgtg tcagttcatt attcactaag ttcacttttg ccgctgccag
     1741 tgctgggatg acattaccat gcattaacat tgtgatgggc aacggaccgt gctcaacatc
     1801 cacatcaaat acaactttct ggcctggctt gatattgaga tcgggtgcac cgttatcgaa
     1861 tgtcatttgc atttgaaaac gggaattaaa aaagcctttg tcaaagtttt tataacttaa
     1921 cgtcatatcg taggcatgtt cacctttctt tttcgcatcg ttaaaatctt taataaactt
     1981 ttcgatacca ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc agattacggc
     2041 taacgcaaca ataacacctg tagctgactt acgtatcata taaatattcc ctgataaaac
     2101 agcaaaactt tgaatatcca cttatgctga aataatgtcc agcg
//
LOCUS       DAZFJF010000313         2138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFJF010000313 DAZFJF010000000
VERSION     DAZFJF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.315"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..2129
                     /gene="yjcS"
                     /locus_tag="ACHKAU_005268"
     CDS             144..2129
                     /gene="yjcS"
                     /locus_tag="ACHKAU_005268"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO5534125.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          573 a          563 c          524 g          478 t
ORIGIN      
        1 acaaatgcct aataaaatgg atcgttccgc tcacacttcc acttattaaa gtcgcaaatt
       61 cccaattatg ataacgccag ctttaaatga cgtattttgc atggattaca tatttacgac
      121 acaaatgata aatggagatt tatatgaata actctcggtt attccgtttg agcaggattg
      181 ttattgcgtt aactgctgcc agcggcatga tggtaaatac cgctaacgcg aaagaggaag
      241 cgaaagccgc cactcaatat acccaacagg ttaatcagaa ttacgccaaa tcattaccgt
      301 ttagcgatcg tcaggatttt gacgacgctc agcgtggatt tatcgctccg ctgctggatg
      361 aaggtattct gcgcgatgct aatggcaaac catattatcg cggggaagat tataaatttg
      421 atatcaatgc gcccgcgccc gaaaccgtta accctagcct gtggcgtcag tcgcagttaa
      481 acggtatttc cggcctgttt aaagtcaccg acagaatgta ccaggttcgc ggtcaggata
      541 tctccaacat cacctttatc gaaggtgata ccgggattat tgtcatcgac ccgctggtga
      601 cgccaccgag cgcaaaagcc gcccttgacc tttacttcca aaatcgcccg caaaaaccga
      661 ttgttgcggt tatttatacc cacagccata ccgaccacta tggcggcgtg aaaggcatta
      721 tctctgaagc tgatgtgaaa tccggtaaag tgcaggttat cgcccctgct ggctttatgg
      781 acgaagccat cagcgaaaac gtactggcgg gtaatattat gagccgccgt gcactttact
      841 cctacggcct gctgctggcg cataaccctc agggtaacat cggcaatggt cttggcgtaa
      901 cgctggcatc gggctacccg agcatcatcg caccgaacaa aaccatcacc aaaaccggtg
      961 agaagatgat tatcgacggc ctggagtttg acttcctgat gaccccaggt agcgaagcac
     1021 cagccgaaat gcacttctat attccggccc tgaaagcgct gtgtaccgcc gagaacgcca
     1081 cgcataccct gcacaacttc tacactctac gcggcgcgaa aacccgcgac accagcaagt
     1141 ggaccgagta tctgaacgaa acgctggata tgtggggtaa cgacgcggaa gtcctgttta
     1201 tgccgcacac ctggccggtc tggggcaata agcatatcaa tgattatatt ggtaaatatc
     1261 gcgatactat caagtacatt cacgaccaga ccctgcacct ggcgaaccag ggctacacca
     1321 tgaatgaaat cggcgacatg attaaactgc cgcctgcact tgccaataac tgggccagcc
     1381 gtggctatta cggttctgtc agccacaacg cccgcgcggt gtataacttc tatcttggct
     1441 attacgacgg taacccggct aacctgcatc cgtatggtca ggtggagatg ggtaaacgtt
     1501 acgtgcaggc gctgggcggt tctgcccgtg tcatcaacct ggcgcaagaa gcgaacaagc
     1561 aaggtgatta ccgctggtcg gcagaactgc tgaaacaggt gattgccgcc aacccgggtg
     1621 accaggtcgc gaagaatctg caagcgaata actttgaaca gctgggctat caggccgagt
     1681 ccgccacctg gcgcggtttc tacctgaccg gcgcgaaaga gctgcgcgaa ggggtgcata
     1741 agttcagcca cggcaccacc ggttccccgg acaccattcg cgggatgtcg gtcgaaatgc
     1801 tgttcgactt tatgtccgtt cgcctcgata gcgcgaaagc cgcgggtaaa aatatcagcc
     1861 tgaacttcaa tatgagcaat ggcgataacc tcaacctgac gctgaacgat agcgtgctta
     1921 actaccgtaa aacactgcaa tcccaagctg acgcctcttt ctacatcagc cgtgaagatc
     1981 tgcacgccgt gctgaccggg caagccaaaa tggcggatct ggtaaaagcg aagaaagcca
     2041 aaattattgg caatggcgcg aaactggaag aaattatcgc ctgtctggat aatttcgatt
     2101 tgtgggtgaa tatcgtaacc ccaaattaat gtcactcg
//
LOCUS       DAZFJF010000314         2121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFJF010000314 DAZFJF010000000
VERSION     DAZFJF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.316"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1470)
                     /locus_tag="ACHKAU_005269"
     CDS             complement(<1..1470)
                     /locus_tag="ACHKAU_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFO5534126.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQ"
     gene            complement(1559..>2121)
                     /locus_tag="ACHKAU_005270"
     CDS             complement(1559..>2121)
                     /locus_tag="ACHKAU_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrmB family transcriptional regulator
                     sugar-binding domain-containing protein"
                     /protein_id="HFO5534127.1"
                     /translation="STIDNIKILKKELSQMMFQNEREFIYNVSGFDNLHQKAKEMLSS
                     AECEIYLNTDFDLHFLKNEICEALNRHVRVIVFSFNKLITPDPRVELYYRFNHEEEYY
                     PSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLLINIIAEHIHSDIYLTKYQQQYPDAN
                     ALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          673 a          404 c          373 g          671 t
ORIGIN      
        1 ttgagtatca tttttagtaa aagtaacacc attaataatc atcatttttg cccagccggg
       61 gtaacggaaa ctgagagcaa aacgaacaga tgactcaggc tcaatgagac attttacgct
      121 taaatcatga gggtattcag ttaattgtgt aattttaatt gcaacattat taaatgtgcc
      181 ataaaactca cacgggccat aaaataatgc agtaaaacca ttgttatttt cttgaaacat
      241 attttcgaca aaagccggta tggttcttgc tgtattcggt acgcagcaga cagcagcctc
      301 ctcatgagta ggggagtatt tatatctggt attaaactga ttactgtccg gatgtttcct
      361 ttcatttgct tcataacaat tatcagtctt acagtacatt atcgcgtgaa gtatgggatg
      421 ctgcatgcca aaactagcat tatagaatag ccactcagca tcatcggcca gaccaatatt
      481 agtggtcagt tttattaaca taacataagt gctaaatagt tcatgaacag aacaatattc
      541 atagcctgta taagtggcat tagctgtgcg tcctgtaatc cactcatcac caatgggagc
      601 gccagatggt gtaagataat atttcaactg ggataaaaaa atatctaatg cagtttgata
      661 ctgcggccgt tggctcgctg cgatggctaa tgccctaata tgctcatacg tatgtacgcc
      721 atgaccttta agaaggtaac cagggtctat gacattttta tattgcagat cttgctccga
      781 cacgcatcct gcggaataat cttcatacag ccacaagcaa tatgagagat attcaatgtt
      841 attggttatt tcataaaggc ggtagaaaac atctgttatt gttaatccgt ggctatgtcc
      901 agcaaaaccc tgttccacaa cgaaaggatg tgacgtttct ttagggtagc cagccataat
      961 gcattgtgcg gcttgggtaa gagaagttaa taaactctca ttacgggtgg actcataaca
     1021 tccaagcaca aaacgcaagg cagtagcttg tgcccagaac tctccatttt ctgctttaca
     1081 gttaaatttt aatgatgaat cataaattcc aatatagcca tcatcatctt gcgtatcgat
     1141 aagttccaaa cataaatcgc tcgctttttc ctgccattct ttattatcta acaagtaggc
     1201 actgcggcaa tatccatcct tccaattcga ttgagtttca ctatcccacc aataatattg
     1261 catcatgtta tcatcgggat tattctgttt tttaataacg ccatcttcag cagatacagt
     1321 gcgtggagtt atacgatctc ttccatatat tttatgctcc gtaataagat ggggaactaa
     1381 tttatccagt tctcctatat gaccgttcat ataccattcc atcttattct ttaaccaacc
     1441 agtgggcttt atatcgttaa aaaaaatcat aaatatacct ttgcataaaa aagtttagac
     1501 gttataaaag acaaatagat tgtgatcgcc tacagacgat cacaatgaaa ggaaaatatt
     1561 aacggttttt gctatcactc aaatgttttt catcctgcac catatcattg ccagattcat
     1621 gcaaagtatt tattaatgca ttggcatctg gatattgttg ctgatacttg gttagatata
     1681 tatcactatg gatatgttcg gcaattatat tgataagcaa gcgattattc gagtataacc
     1741 ccagtgattc agctcgttgt gaaaaaacca ttactgtctt catatcaaca acaagcataa
     1801 aacggtgaga agggtaatat tcttcttcat ggttaaagcg gtaatacagt tctacacgtg
     1861 ggtcgggagt aataagttta ttaaatgaaa aaacaataac tcgaacatgt cgattcagag
     1921 cctcacaaat ttcatttttc aaaaaatgta agtcaaaatc cgtgttgagg taaatttcac
     1981 actctgcaga agaaagcatc tctttagctt tctgatgtaa gttatcgaaa ccagagacat
     2041 tataaatgaa ttcacgttca ttctgaaaca tcatctgtga taattctttt tttagaattt
     2101 ttatattgtc aatagtgctt t
//
LOCUS       DAZFJF010000315         2115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFJF010000315 DAZFJF010000000
VERSION     DAZFJF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.317"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            778..1755
                     /locus_tag="ACHKAU_005271"
     CDS             778..1755
                     /locus_tag="ACHKAU_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO5534128.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            1939..>2115
                     /locus_tag="ACHKAU_005272"
     CDS             1939..>2115
                     /locus_tag="ACHKAU_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5534129.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREG"
BASE COUNT          569 a          520 c          505 g          521 t
ORIGIN      
        1 gtctccagga ttcccggggc ggttcagtgg ataaatgctt attacaacgt ttatcgacct
       61 catacaaaca atggtggttt atcgccttgc cgcatgaaga aaaatggaaa caggttatgc
      121 cggtgtcctg attttatgat ccactacaca ttcctgacga acgccactgc ataaatcaca
      181 ttgcacatcc tcagagagta cgcagtatag gaggggtata tatacattat cggtatatca
      241 ggcgacttct gtctgtatgt tgtcggtgct cgccgcagac cggtgaccgg gcgaagcccg
      301 cgaaccggcg aggaagcgca atgtatctgt agttacagtg agcagatacg ccctgaagct
      361 tcctcataaa acagttgtgt aaactgtttt cccggaatga tttatccaca tatccggcgg
      421 cccgatccag taaacagatc cataaatgat caagaaatga tccattaaag atccccatat
      481 cgctgcaaac cttgtcactc atgctccggg accacgatca cataagctgt ggcatgtttc
      541 tgataaactg tagcaggatc atgataagct gtattcagta atgtatatac tgtagtaagt
      601 taatgacata aattatggtc agtacgccag gctcagctgt taaatacaga ctgcaggttt
      661 ttcttcagtc agttgacggg gttctgacac tcaattttct gtttattctt ttactgtcca
      721 caggcaggag gctttctgga aaacgaaaat tcagacatca aaaaactgtt cggcgaggtg
      781 gataagtcgt ccggtgagct ggtgacactg acaccaaaca ataacaacac cgtacaacct
      841 gtggcgctga tgcgtctggg cgtttttgta ccgaccctta aatcactgaa gaacagtaaa
      901 aaaaatacac tgtcacgtac tgatgccacg gaagagctga cacgtctttc cctggcccgt
      961 gctgagggat ttgataaggt tgagatcacc ggcccccgcc tggatatgga taacgatttc
     1021 aagacctggg tgggaatcat tcattccttt gcccgccata acgtaattgg tgacaaagtt
     1081 gaactgcctt ttgtcgagtt tgcaaaactg tgtggtatac cttcaagcca gtcatcccgc
     1141 aggctgcgtg agcgcatcag cccttccctg aaacgcattg ccggtaccgt gatctccttt
     1201 tcccgcaccg atgagaagca cacccgggaa tatatcaccc atctggtaca gtcagcctac
     1261 tacgatactg aacgggatat tgttcagtta caggctgatc cccgcctttt tgaactgtac
     1321 cagtttgacc ggaaagtgct gctgcagctc aaggccatca atgccctgaa gcgacgggag
     1381 tccgcccagg cactttacac ctttatagag agcctgcccc gggatccggc accgatatcg
     1441 ctggcgcggc tgcgtgcccg cctcaatctg aagtctcctg tattttccca gaaccagacg
     1501 gtcagacggg caatggagca gttgcgcgag attggatatc ttgattacac ggagatccag
     1561 cgggggcgga caaaattctt ctgtattcac taccggcgtc cccggttaaa agcgccgaat
     1621 gatgagagta aggaaaatcc gttgccacct tcacctgcgg aaaaagtcag tccggagatg
     1681 gcggagaaac ttgccctgct tgaaaaactg ggcatcacgc tggatgacct ggaaaaactc
     1741 ttcaaatccc gctgaacata aactgtagtc agtgaagagt gctcctttac tgactacagc
     1801 ttatattatc aggtgcagtg agtggtctgc tcactgcagt ttatatgtaa gcgtacagcc
     1861 tgaaccgtct ggtcagaatc tgacgaatta gacaaagtgg tgtccaccaa ataagtagtg
     1921 ggaaccaaag tgtcagatat gcagaaaaat gtgactcccg gcaggcgaaa aggctgccct
     1981 aattatcctc ccgaatttaa acagcagctc gttgctgcct cctgtgaacc cgggatatcc
     2041 atctcaaaac ttgctcttga aaatggcatt aacgccaatc tgttgttcaa atggcgacaa
     2101 caatggcgcg aggga
//
LOCUS       DAZFJF010000316         2112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFJF010000316 DAZFJF010000000
VERSION     DAZFJF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.318"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..594)
                     /locus_tag="ACHKAU_005273"
     CDS             complement(<1..594)
                     /locus_tag="ACHKAU_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5534130.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRY"
     gene            863..1063
                     /gene="glgS"
                     /locus_tag="ACHKAU_005274"
     CDS             863..1063
                     /gene="glgS"
                     /locus_tag="ACHKAU_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO5534131.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(1106..2112)
                     /locus_tag="ACHKAU_005275"
                     /pseudo
     CDS             complement(1106..2112)
                     /locus_tag="ACHKAU_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          634 a          410 c          447 g          621 t
ORIGIN      
        1 atatcgcgtt gcagaaagtc ggcaaataat taacactttg tctcctttag tgaaaaattt
       61 tccttcttca ggctccagta gtaaataatg tatttggcca aattgatcgg tgagttttcc
      121 ttcgcaggga ttacctgaag tggcttgatg accggtaatt aatgccatgc taccaatata
      181 ttcttcttct gtaatagctg aactatggtc gcgtggaatc cagggcgcaa ctattttacc
      241 ggtataatgc accgcaataa tcgtaaacag taaactgaca ggaaccacga acaggttcga
      301 cagtggtgat tgccagacca tgatgcaggc atgctgcaac aggatgccaa taagaccaaa
      361 aaaaccagcc agcaagcaga gaacgacgag agctggcaat cttccgatat tgagataatg
      421 aagtgcctgg ctaatatgac ctgtggttat ggaatcataa tggtcaagat gtgcatcaag
      481 agcaccggag agcatgtgac cgcagataag cgctaaaatt tctaaaaggc cgatcaacag
      541 tacaaaggaa atagcgaaaa gataaggggt attatagtcg gcgaataaaa tcatatgtca
      601 ctccaatgtc catttagtga cgcaatattt actttgggat agtttccttg cttacattta
      661 tctttgttaa taataacaca tgttagagaa gttctggaag agaaagtttt ttaatatggt
      721 tttttatttt tcactaaaaa gtgtgatcgg ggacaatata tttacgcacg ttatgtttaa
      781 aggcactaca ctgattgggg aatactgaaa tcagaaaaag catatggcct taaaaggtat
      841 gcgataaccg gaggatgtgc ttatggatca tagtcttaat tctttaaata atttcgattt
      901 cctggcgcgt agttttgcca gaatgcacgc agaaggtcgc ccggtcgata ttcttgccgt
      961 tactggtaac atggatgaag aacatagaac ctggttttgc gcacgttatg cctggtattg
     1021 tcaacagatg atgcaggcaa gagagctgga gttagagcac tgatataacg ggcctgatgg
     1081 cccgttttag tgtttgtatt agatgctagt tataggtgat tttgaggatg acgtctttac
     1141 taaacggtcc ctcaacaaca gtctctgccg gattcgcact aagttctgcc ctgaatggtg
     1201 cggtgaccga aggtgcacta gacgatgaaa aagaagcaaa ttgtgagaag cggttaaatt
     1261 ccgttgctgt tccatcttta tgaaatatct tcaatagcag tccattaccc atatccagat
     1321 ggctataatc actcaaaata tccgtagtga aaaagctccc taaaatattg aattgctcag
     1381 tacaggccgc accaggatct tttgtcatga ctacactaaa cgtctcacta acgctggtat
     1441 ttttaattgt tcgggaattt attttaccaa agtcaacaac gccatcagct ggttccagtg
     1501 cgacattaac aaagcagtca aggacatgaa tattatttaa tccacgaatc ctgaaggtca
     1561 aatttttgtc tacttctata gctcctgcgc cactcatacc tttaaactct agagcgatat
     1621 attcatcagg taacaccagg tttttattaa ctctattggc attttttgct aatttaatat
     1681 agagttgtac gcgagctgga aaggttactg ataattgagg ctcaggacct ccactacaga
     1741 cagcggggtt ttttatagtg attattaaag gtacccgctc cttttatatc aatgcattct
     1801 ggtgctgcga tagcattagg ggcagtcgaa tcataatcaa ctccattgta tgttacgcca
     1861 aaaatatagg agttctgatt ataaaagtcc tgaccgcctg gtaaaaacat cattatatta
     1921 gtccaggctg atgggtattc ttctccaggt gatggggcag gtaatgcatt attacagaag
     1981 acattcataa aatactcact gctttgccag atcttatcac cttttttacc attactgggg
     2041 atagaaattc ttggtacgta accaatgact acggtatttc ccccttcatg ttcagcatag
     2101 cagtccattg cc
//
LOCUS       DAZFJF010000317         2100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFJF010000317 DAZFJF010000000
VERSION     DAZFJF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.319"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..570
                     /locus_tag="ACHKAU_005276"
     CDS             37..570
                     /locus_tag="ACHKAU_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5534132.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            863..994
                     /locus_tag="ACHKAU_005277"
     CDS             863..994
                     /locus_tag="ACHKAU_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534133.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVALFLSMGWMMLSSSGLLG"
     gene            1217..1423
                     /locus_tag="ACHKAU_005278"
     CDS             1217..1423
                     /locus_tag="ACHKAU_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5534134.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            1488..1712
                     /locus_tag="ACHKAU_005279"
     CDS             1488..1712
                     /locus_tag="ACHKAU_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534135.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          527 a          452 c          565 g          556 t
ORIGIN      
        1 ctaacatatc aagggattag aaatcgataa atccccatga acgaaaaaat aaaatacggc
       61 ctgtcggctg ccgttctggc gctgattggt gcaggtgctt ctgcgcctga aatcctcgac
      121 cagtttctgg atgaaaagga aggtaaccac accacggcat accgtgatgg tgcggggatc
      181 tggaccatct gccgtggtgc cattctggtg gatggtaagc ctgttattcc tggcatgaag
      241 ctgtcaaagg aaaaatgcga ccgggttaat gccatcgaac gtgacaaggc gctggcatgg
      301 gtggagaaaa acatcaaagt gccgctgacc gaaccccaga aagcggggat cgcgtcattc
      361 tgtccgtata acattggccc cggtaagtgt ttcccgtcga cgttttataa acgaattaat
      421 gcaggtgatc gaaaaggtgc ctgcgaagcg attcgctggt ggattaagga cggtggcaga
      481 gactgccgta tccgctcaaa taattgctac ggtcaggtat cccggcgaga tcaggaaagt
      541 gcgctggcgt gctggggtat cgacagataa gcagaatatt ttgcttaaaa atgacgttgg
      601 ccaacgcggg cagataacac gaaatcctgc gaactggcaa aacccaagtg aataaaagta
      661 aaaaccccgt ttgttggttg caaacggggt tttatgttta tggcagtaag ctatgggagg
      721 ctgccttgat tgattttagc aaactgatta gggagttgcg actcatgatt agtcaattac
      781 caaactggaa atttttgctg gtctggagca tccctttttt atgggtagta tcccagtgaa
      841 ttgtggcaat taaggggtag ctatgtcaga caaactcata atgctggcga agatcctctg
      901 tgtaatcgtc ggcatttcat tttcactaat gctggttgct ctttttcttt ccatgggctg
      961 gatgatgttg tcttcgtcgg ggctgctggg gtgagcataa accgaatgct ttccgcgttt
     1021 accgttattc tgctggtggt ctgtggtgcg cttagtctgg ggctgaatca ttaccgcgat
     1081 aacgccatca cctacaaagc gcagcgcgat aaaaaagcca gtgagctgga tctggcaaac
     1141 gcaaccatta ctgatatgca ggtgcgccag cgcgatgttg ctgcgctcga tgcaaaatac
     1201 tcgagggaat tagccgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct
     1261 ggtcgtaagc gcctgcggat caacgccacc tgccccggta ccgtgcgtga agccaccggc
     1321 acctccggcg tgggcaatga tgccgccgtc gaactctctc cggttgctgg acgaaacgtt
     1381 ctcggtatca gagacggaat catcagcgac caggcagcat tgagaatgct tcaggaatat
     1441 atccgcactc agtgtattaa ctagtatttt tgttattcgg agaatgcatg aagaaattat
     1501 tggtaaccgt aaagcctttt cagggaacaa ttccgttccg tattttgcag cgtggtcgtg
     1561 ttcttgttga aggttcgttc agtggtaaat gtacgcaatt acactcccgg acctttcagg
     1621 tgaatgccac gaatgaagag ctaaccgttg agtgtacgat gaatgccgct aaatgccgca
     1681 tggtatccgc tgcattacag ccagtgtgtt gagcgacctt attattcatg cgcggtattg
     1741 tcgccgtatt cctgcattaa cagagaccgc agcccgaccg ggagaatcct ctgcgcgagt
     1801 gtgcggggat aatcaaaaac gatacacacc ggggtttacc gcgttaacgg agcgcggcgt
     1861 tgtcccctca tggtcgctgg tccggtgcga tggtggaaga agccggatgt ttatcactat
     1921 taattgataa cacagaaatg gattcattga ttttcagcac gtttttgtat tcgtattatt
     1981 gaacatctgt ttattttact tttaacatat tgataataaa aagagctgta aatctttaga
     2041 tgagtcgatt ttgtccgggg aagttcaaat ggattttatg ctgacggttt ctggtgtggt
//
LOCUS       DAZFJF010000318         2093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFJF010000318 DAZFJF010000000
VERSION     DAZFJF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.320"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..275
                     /locus_tag="ACHKAU_005280"
     CDS             6..275
                     /locus_tag="ACHKAU_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5534136.1"
                     /translation="MNILKKLMQRMCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRGDGTWENIGGMK"
     gene            complement(392..667)
                     /locus_tag="ACHKAU_005281"
     CDS             complement(392..667)
                     /locus_tag="ACHKAU_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO5534137.1"
                     /translation="MPFSIKNIFSNSKGSYPEISGPVQDKPVSKNCTLTSTTCSMNDY
                     TVFSRKSCTFDMRPPGAGDRTPQLKLSASELIWLSKTIDTERNNIKE"
     gene            complement(730..2091)
                     /gene="espK"
                     /locus_tag="ACHKAU_005282"
     CDS             complement(730..2091)
                     /gene="espK"
                     /locus_tag="ACHKAU_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO5534138.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSAGIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTILTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIIPEGIECLTVCRCHISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLQTLSLTGCSNIILPEILPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKSH
                     NNYNVTMADLCVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADEIVNSLKLIANKSDG
                     YGQSITASELRWIYRNKDNDQIMKNIKFYLRGKEIPAEKILGAPEWKDYKPKYSGATY
                     KYS"
BASE COUNT          486 a          398 c          500 g          709 t
ORIGIN      
        1 atgacatgaa catactaaaa aaacttatgc agcgtatgtg tggttgcgga aagcatgatg
       61 gccgtgaaca cgtgcagtcg cttacagcac agctgcgact gggaccggca gacattctgg
      121 agtccgatga gaatggcatt atcccggagc aggacagggt aatcacgcag gtggtgatac
      181 tggatgcgga taaaaagcag atacagtgcg tggtaagacc gctgcaaatt ctgcgtggtg
      241 acgggacgtg ggaaaatatt ggcgggatga agtaactcga cagcttcaca aaaccggagt
      301 ccggctccat tttaccacct ccacgggagg tggttgcttg caatcagatg tttcgggcct
      361 gcggcttttt gtgttcccta gtaagtggtt actattcttt tatgttattc ctctctgtat
      421 ctattgtctt agatagccat atgagttcag atgctgagag ttttaattgt ggtgttctgt
      481 ctcctgctcc gggtggacgc atgtcaaaag tgcaggattt tctgctgaat actgtgtagt
      541 cattcataga gcatgttgtt gaggttagtg tacaattctt cgatactggc ttgtcctgca
      601 caggtccgga tatttctggg tagcttcctt ttgaatttga aaatatgttt ttgattgaaa
      661 atggcatcgt gctttgtcct ttgaactacg atatattgtt ttgtgttctt tcgttgtgag
      721 taggtatcat taagaatact tatatgtggc tccagagtat ttgggtttgt agtctttcca
      781 ctctggtgcg cctaatattt tttctgctgg tatctctttc ccacgcaagt aaaactttat
      841 gtttttcatt atctggtcgt tgtctttgtt gcgataaatc catcgtaatt cgctggcagt
      901 aatactctga ccataaccat ctgacttatt agcaatgagt ttgagtgagt tgacaatctc
      961 gtcagcacaa aaaataaccg ttttgttgcg tgttaatgtc tgaaactcca ggccggcctt
     1021 gcttgttttt gcccataatt tgtcgcgatt gtatacgcaa aggtcggcca tggtgacatt
     1081 ataattattg tggctcttta ggtgttgttt aaattttatc cctctttcgt gttcatttaa
     1141 tgctcttttg atcagtggat cttgcttata tttgcgaggt gttctggggt cccatactgc
     1201 gtttgttaac gtattctgag ttataatatc ttttttggaa aagtcgttaa ctagtttaat
     1261 gctgttgaca agtttttctc tgggtgaaga tagtccatag acaatgtctc cctcgcgaaa
     1321 gtgtaaggcc acatccagag cgttgccctg aaaggtgatg ttttttgctt taactacatc
     1381 tggagagagt agtacatttt gtaaatcaag atccagccca tcaggcatcc cttcaacacc
     1441 gatgttccac gtggttttct gctccgcatg aatggttacc gatgtcacac tctccggaag
     1501 tatctccggc agtataatat tgctacatcc agtcagcgat agcgtctgca gtgacggtga
     1561 tatcaggttg tcaattctgg cctgattctc cgggttatag ctattgatgc tgagagatga
     1621 taacccgttt ggaacatttt ttatgctgtc tgtggcgcta ccttttatct caaggtagcg
     1681 gacgctctct ggcactccag atatatggca gcgacaaacc gtcaggcatt cgatgccttc
     1741 aggtatgatg tgtataaatg gtgaatcagt taattcaata tgtagatttt ttactgtcga
     1801 tggtatgtct gtgatttgta atttttcaca gttaatgacg gagaggtttt gtgtattaac
     1861 cggcaatcct gtaaggattg tcaggttgtt gcagttttcg attgtaactg atgttatttc
     1921 gtttggtaac tgtggcagat actcaatatc acaatctttg atatataaac gaccactggc
     1981 ccgcgcttct tctatctgaa ttggtgtgat ttcgcttttg atgcctgctg atgtttcagc
     2041 ggaataggag cagaatgtcc cggtcggtcg taattgcgat gtaggaagca ttg
//
LOCUS       DAZFJF010000319         2077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFJF010000319 DAZFJF010000000
VERSION     DAZFJF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.321"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1659)
                     /locus_tag="ACHKAU_005283"
     CDS             complement(<1..1659)
                     /locus_tag="ACHKAU_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5534139.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQE"
     gene            complement(1640..>2077)
                     /locus_tag="ACHKAU_005284"
     CDS             complement(1640..>2077)
                     /locus_tag="ACHKAU_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5534140.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLD
                     MHFSGLTYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVR
                     FGGDGGRDILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
BASE COUNT          414 a          680 c          515 g          468 t
ORIGIN      
        1 ctcctgatac gtaaccttgt cgccaagtgc agccagctgg cgtttgtact ccagcgtctc
       61 atctttatgc gccagcaggg atttctcctg tgcagacagc tggcgacgtt gcgccgcctc
      121 ctccagtacc gcgaactgac tctccgcctt ccacaaatcc cggcgctgct ggctgatttt
      181 ctcatttgct ccggcatgct tctccagcgt ccggagttct gcctgaagcg tcagcagggc
      241 agcatgagca ctgtcttcct gacgatcgcc cgcagacacc ttcacgccgg actgtttcgg
      301 ctttttcagc gtcgcttcat aatccttttt cgccgccgcc atcagcgtgt tgtaatccgc
      361 ctgcaggatt ttcccgtctt tcagtgcctt gttcagttct tcctgacggg cggtatattt
      421 ctccagcggc gtctgcagcc gttcgtaagc cttctgcgcc tcttcggtat atttcagccg
      481 tgacgcttcg gtatcgctct gctgctgcgc atttttgtcc tgttgagtct gctgctcagc
      541 cttctttcgg gcggcttcaa gcgcaagacg ggccttttca cgatcatccc agtaacgcgc
      601 ccgcgcttca tcgttaacaa aataatcatc cttgcgcaga ttccagatgt cgtctgcttt
      661 cttaaacgca gcctctgcct taatcagcat ctcctgcgcg gtatcaggac gaccaatatc
      721 cagcaccgca tcccacatgg atttgaatgc ccgtgcagtc ctgtctgccc aggtctccag
      781 cgtgcccatg ttctctttca ggcggcgggt ctgctcatca aagcctttcg tggcgatatc
      841 gttcgccgcc tgtaaggccc cggcctcatc accggaacgc tgcagctgcg caacatacgc
      901 aatctgctct gccgtcacgt tacggaactg gcgcgccatc gcaatcagcc ccgacgtagg
      961 gtcggtggtc agttttccga aagcctctgc aaccttgtcc acctccacac cggatgcaga
     1021 agcaaaacgc gcgacactct ggttgatggc atcaaactgt tcaccaccac gcacaccggc
     1081 attcaccagg gctgccagtg actcactcgc ctggttaaac gtcagccctg cggcctgtcc
     1141 ggctctggag agcgtcaaca tgcgatcggc agtcagtccg gactgattac cggaaagaac
     1201 cagggtttta ttaaacgctg aaagcgtgga atccccctgg taccaggcgt acgccagcgc
     1261 acctgtcgcc accgccagcg aggtgacccc gaccatcggc agggtgatcg caccggcaag
     1321 ccccctgaac atggggatca tcccgccgaa ggagtccttc acctgaccgc cctgttgcag
     1381 caggatcagc cagggattct gaccaccggc aagctgcgtg gcgatatccg taaactgtgc
     1441 gggcagggtt cgcatggccg ctttatactg cccgacggaa atcccggctt tttgtgcagc
     1501 cagcgcctgg cggctcaggc cctgttcaac agcactggcg gtttttctgg cgtcggtatc
     1561 cagacctgaa aaatgacgcc ttacccggct catctgctca tcgaaacgga ccgcatccag
     1621 actaaggtca ataacaagat caccaaccgg ctgggacata tctcacacct ccggaaatcc
     1681 ccgctgaagc catcattaat gcggcatcat ccaccatgac atccgccaca tccgcagacg
     1741 ataaaatatc gcgccctccg tccccaccga accggacgcc tccggcaagt cctgccgctt
     1801 tctgcatcag cattttgtcc tcatccggcc tctccacctg ctcttcctca tgccggggga
     1861 caagcagact gaaatcagag ggatgcatat ccggatcgca aaaaaacagg ctgagtacag
     1921 cgtacgtcag cccggaaaaa tgcatatcca gctgggtatc ctgaaaataa tgcgtgcggt
     1981 aaaaacggtg ccagtcggca tattcggtgg atgtcatccc ggcaagcatg gcgcgccagt
     2041 cgggtctccc catctcacgc gccagtctga gggcaaa
//
LOCUS       DAZFJF010000320         2065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFJF010000320 DAZFJF010000000
VERSION     DAZFJF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.322"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..109)
                     /locus_tag="ACHKAU_005285"
     CDS             complement(<1..109)
                     /locus_tag="ACHKAU_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5534141.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKASAMT"
     gene            complement(119..1438)
                     /locus_tag="ACHKAU_005286"
     CDS             complement(119..1438)
                     /locus_tag="ACHKAU_005286"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5534142.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAM
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(1419..>2065)
                     /locus_tag="ACHKAU_005287"
     CDS             complement(1419..>2065)
                     /locus_tag="ACHKAU_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5534143.1"
                     /translation="AAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIA
                     AGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLEEAIV
                     RRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEKEC
                     AKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          380 a          661 c          584 g          440 t
ORIGIN      
        1 gggtcattgc agacgctttc gcactcaatc cgccgggcgc ggttgcggta tgagccgggt
       61 cactgttgcc cagcggctgg taatgggtaa aggtttcttt gctcgtcata aacatccctt
      121 acactggtgt gttcagcaaa tcgttaacgg catcagatgc cgggttacct gcagccagcg
      181 gtgccggtgc cccctgcatc agacgatcca gcgcagtatc actgcgcgcc tgtgcactct
      241 gtggtgctgc ggccagaatg cggcgggccg tttccacggt cataccgggg gtttctgcca
      301 gcacgcgggc ctgttcttcg cgtccgtgag cctcctcaca gttgaggatc cccataatgc
      361 ggctgttttc tgccgcaacc gcagcggtga tctgcgcgtt cacgtccggc tgcgccgcgc
      421 tggcgttttc gccctccatc gctggcacca cgccagtaac gtcagcctgc gaagcagtgg
      481 ctgaaacagt tgttgattga gtctctttgg tcattcgccc tcctgagaga cgggatttac
      541 gtgcatccag tgcatcacgc ataacggtga tcgcatcggt gctgttgaca agttcatcag
      601 ccagtccggc atcaatggcc tcctgaccgc tgtacactgc agcctcggta tccagcacag
      661 cctgcacgga caggccggta tatgccgaca ccttctgcgc aaacatccgg cgggttgcat
      721 ccatccggga ctgcagtgtt tcccggacat cacccggtag atggctgtag gggttgccat
      781 ccaccttatg gctgccgctg taaatcagcg tgatttccac gccctgtttc tccagggcag
      841 caccgtaatt actgtgagcc atcatgacgc cgatggagcc tgtccgggcg gtctgcgtga
      901 ccagacgccg ggaggcggcg ctggcaagca gctgacctgc actgcagttc atgtcgttgg
      961 ccagcgccca taccggtttt atgtcacgca cacgggcgat gatgtcagca cagtcaaatg
     1021 ctcccgccac cattccgccc ggtgtgtcca tatcgagcag aatgccgtcc accatcgggt
     1081 cgctggcagc ctgttgcaga cgggcgataa tgccgttgta accggtcatc cccgaatacg
     1141 gctgcagcgc ccgcgtccgg ctgaccagcg taccggacac cggcagcacg gcgatgccgt
     1201 tcatgacctg ataactccgg gcctgtcgtg gtccgtcatc atcaccggat aacgccagcg
     1261 ccgcgggtgc ctctccggca gtcaggctgt cgccggatac tgcatccgtc aggcggctga
     1321 tcccaagctg gcctgcaagc gcacaaaaga aaacccgcgc ataggcgggt tcaagcatca
     1381 gcggctcatt aaaagccatg ctggcaatat gcgggagatt acgcagctct gctgtcactc
     1441 ttctcctcct ctgttgattg tcgcagcccg gattcaaatg ccgcagccgc ccaggcgggc
     1501 ggtttaagac cggctgcgcg gcgctccatc gtttcacgga cctgctgggc aaaaatttcc
     1561 tgatagtcgt caccgcgttt cgcgcactct ttctcgtagg tgctcagtcc ggcttctatc
     1621 agcatcaccg cttcctgaac ttctttcaga ccatcgatgg ccatacgacc ggagcctatc
     1681 cagtcgcagt tcccccaggc actgcgggct tcctgaaagc tgaagcgcgc ttttgaaggt
     1741 aacgtcacca cgcggcgaac gatggcctct tccagccagc acagaaacat ctggctcgcc
     1801 tgacgggatg cgacgaattt tcgccgcccc ataaagtacg cccacgactc gttcgcactg
     1861 gcccgtgccg tggagtagct catctgggcg taattccggg aaagctgctc atacgagaca
     1921 cccagcccgg cagcgatata ccgcagcagt gactgctcaa acacggagta gccgttatcc
     1981 gtgtcctgag ccgtctgcag gttcagtgag tcccccggca tcaggtgcgg cacttttgcg
     2041 cctcccagac ggaccggtgc tgcgg
//
LOCUS       DAZFJF010000321         2031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFJF010000321 DAZFJF010000000
VERSION     DAZFJF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.323"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            397..663
                     /locus_tag="ACHKAU_005288"
     CDS             397..663
                     /locus_tag="ACHKAU_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5534144.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            660..1259
                     /locus_tag="ACHKAU_005289"
     CDS             660..1259
                     /locus_tag="ACHKAU_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5534145.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            1252..1539
                     /locus_tag="ACHKAU_005290"
     CDS             1252..1539
                     /locus_tag="ACHKAU_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFO5534146.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            1532..1987
                     /locus_tag="ACHKAU_005291"
     CDS             1532..1987
                     /locus_tag="ACHKAU_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534147.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQEPVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
BASE COUNT          449 a          556 c          513 g          513 t
ORIGIN      
        1 tttttatatt ttactgttca cctctgttca ccataagaaa aaagataagt aatacagtaa
       61 gttaaagggt gaacaatcgc agtaatgact gttcaccgtc tgttcaccac tgttcacccg
      121 ttgatgggct ttttgtgctg tttattactt tttattttta ttaattcact gaaataaata
      181 agaaaaaaca atttgtattt cactataatt ttttccagct ccttccagag cgttttaaag
      241 ctatctggcg ctgatgtaca aaaaatacac agccattgta aggctgccgg aacaaattcc
      301 ccctgttgcg tctgccgaaa atattcacaa aataaagcgc tacccgaagc cggacggact
      361 tatccggtgc tgtatggaca ttaacgaggt agcccgatgc aagctgtttt ttcttccccg
      421 tctcccgccc cagtgacgcc actgatgccg ctgccggaca tcacgcagga gcgtttttta
      481 cgtctgccgg aagtgatgca cctgtgcggc ctgtcacgct cgaccatcta tgaacttatc
      541 cgtaaggggg aatttccgcc gcaggtgagt cttggcggta aaaatgtggc ctggctgcac
      601 tctgaagtca ccgcatggat ggccgggcgc attgccggac gcaaacgggg gtacgacgca
      661 tgatgatgcc cgctctgcaa aaactccctt tttctggctt gcctttttcc ggcatttgcg
      721 gatatagttt ttccgctgcc gcaaaatcgg cagccgggcg tgagaacccg agcaattcac
      781 aggcgacata tgacgcgcca cgcgtctttt tttgtgtcgc aatcaatgcc acagagcgcc
      841 agattatggt gtggtgtgtg gtcagtcgtg cagatggcat tccgtgcatc ctgcccgcgt
      901 ccgctcatta tgccgcagag tcaatggtag ctcaggcggg gcagcctccg ggctggccgg
      961 tatcctgtga agccggtatt ctcacccccg tctgggctat cgccatcgag cgtgagaact
     1021 ccggcgatag cgttatttgc tattcacagg aggctgccat catggctaca acccttaccc
     1081 cctcacaccc tgaatttgtc tttgtgtttg cggctgtccg tcgcgcagac cgtcatcccc
     1141 gtatctgtat gcttcgcacc gtcgccggtg atgaacgcag tgcccgccgt tcccttgtcc
     1201 gtgactatgt gctctccctt gctgcccgtc tgccggtggt ggaggtgtcc cgtgcgtaat
     1261 aaaaaagccc ctcagaccgt ctcagcacgt catgacgccc gtgaacacct cagcattgag
     1321 gcttaccata agctcaaccg cgccagcgcc gtatcccagt ttgttggggg tgatttgatt
     1381 caccgtgaac tctccggcct gcatcagctc tacattccgc atattttcag ctacctgaat
     1441 gaagatattg attttgtgct gaatgagctg aaagccaaag gcctgtgccg cgattttctc
     1501 gcccagcaga aagaccgggg agacaggacg catgtttgat tttccccagc ccggtgagat
     1561 ttaccgttct gccggttttc ccgatgtggc cgtggtcggc attctggaag acggtattcc
     1621 gtgggaaatg ccgtaccgct gcccggaaat tgtctggaac ccgtaccgcc gtaaattcag
     1681 tatccttgtg cgtatcctcg ctgacgggcg caccacagac atcccgctgg ggcgttttct
     1741 gcgggaattt acctgtgacc gtcctgacct gtttaaacgc agccccgtaa accggcatgc
     1801 ggtactgaaa gaaatggccg gagacccgga attacagaaa tggcgggaga aatatctgga
     1861 tatttacccg caggagcctg ttccggtcag ccgggcggca ccggtggcgc gggaatggcg
     1921 ggaaattccc cgcacggagc ctgacccgga aatcaccccg gataacagtt accgcaatta
     1981 tctgtaatta aaaaacgaca cccgaaaaaa aaattaaatg tgcgtattcg c
//
LOCUS       DAZFJF010000322         2023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFJF010000322 DAZFJF010000000
VERSION     DAZFJF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.324"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(68..259)
                     /locus_tag="ACHKAU_005292"
     CDS             complement(68..259)
                     /locus_tag="ACHKAU_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5534148.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHKAU_005293"
     CDS             complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHKAU_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5534149.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            complement(934..1086)
                     /locus_tag="ACHKAU_005294"
     CDS             complement(934..1086)
                     /locus_tag="ACHKAU_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5534150.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            complement(1363..2007)
                     /locus_tag="ACHKAU_005295"
     CDS             complement(1363..2007)
                     /locus_tag="ACHKAU_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5534151.1"
                     /translation="MESLGIRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
BASE COUNT          542 a          467 c          437 g          577 t
ORIGIN      
        1 agggtgaaca aatccctgcc atttctggca tataagaatg aaatcggatg tttattacgg
       61 aactgtttta aagacctgcc gggatttcgt tattatcctg gtgaataact ttatcgacag
      121 ggtaacagtt accgggaatt ttctgttcgg ttgctgcagt cacacactcc tgcattgtcc
      181 tgtgaacact gactgcaata tcaactggct ctccggaaac aagaaaaact gtcagaacaa
      241 gtgcaaatgc tgtattcatt gccagcatcc tttttgtatc ggacgtaaac gggccagcat
      301 tgaaagaatg catattttat ttaataactc ccgttcgtgt tttctcttgt taatggcatc
      361 ttcagtaaat acagggttac tgatagtgac accaatttca aaacaacctt cagacgtatt
      421 aacgtttggt aataacgttt ccattatcgc gtcctcaaca atgaattttg tgatgcagtg
      481 cctggtgcct ccaggtgacg ttaaccagtt aacaattaac gccggataca gagaatccac
      541 ccataacact gtttttggtt ttaactgttc cgcgtgcgct cagccgcatt caccgcatca
      601 caaaattcac tttaaaaagg gcggcagagc agtcacggag taaaactgat accgccaaac
      661 gtcaccagaa aattgataac agagggcgtt gctgcggggt tgtcacttaa gcgtatggtc
      721 aacctgacaa cccggtgtcc tcaatggggg aaggaataac cccgccatac ttaccgccgc
      781 gccatttcgc ggattgccac aaccagaagc gcacggtcga agaaatttaa cgacaagact
      841 tatatgcaaa gggatctcgc cgtgcgcttt cgtgttatgc cctgactttt cagggtaaat
      901 taacctgtgg aaacctgttt ttactggcgc taatcagtta gcgtttcttg ctaaccagcg
      961 atgcgcgcca gcttcggttt taaacgtttt acttctggta tacgtcatcg cggtaaacgt
     1021 gccgtcgtgg ttggggaata ctccgcacac cagagattca ttgttgccaa gattgagcat
     1081 atccatgttg acctcattta cccttaacgc cgggtcgcgg aactaaaaac ctgctgcgct
     1141 gttatacaaa gtgttcccgc cgtcatgttc atacgcctcg ggctggctac ttaacccctg
     1201 accactgccg ggtaactcga ggtattgccc gacgttctgt ggggcgaggt agggtggtat
     1261 gtaataaatc taaaagtatt tagtttttca gtcaagataa atctagatta atttaaaaaa
     1321 aaaaggtcgg cattgccgac ctcaagtaaa ggacgtggcg ggttataagt tgaactgtac
     1381 accttttgct acggcaacaa ttttacactc tggtgtaagt aaggatgact gatagcgtgg
     1441 attgagaggg cttaaataca caagcttccc atcaataact aatttcttta tagtcataga
     1501 tggttcattt ttaagcggat ccggaactat tacagcgacg atgctgccat ttttataact
     1561 ttctcctggc cttaggatca cagtagcacc aactgggata ctcggtgatc ctgatgggtt
     1621 atgcattgtg tcatcaggca ttgaaacggc aaaatcacct tcctcgacat caaagaatgt
     1681 ggtgatccta tcgacatttc ccattgtttt ctctcctttt aagattagga aagaaatcgc
     1741 ctcaccccac gaaaggtagg ggatcttggt gcctgaggta ctttgtacaa aagataattc
     1801 aggagacgat actccatata ggagatagga ctcagtagtt cctaatgcct gggctaactt
     1861 acttagagct ttgctacctg gttcgtttag atctttctcc cagtacccta tagtaacccc
     1921 agtcacgcct gaaagcttac ccagttctac ttgggtcagc cccttatctt ttctaagtct
     1981 cttaagcctg atgccaaggc tttccatcat tttctcctgc aaa
//
LOCUS       DAZFJF010000323         2014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFJF010000323 DAZFJF010000000
VERSION     DAZFJF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.325"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2014
                     /locus_tag="ACHKAU_005296"
     CDS             <1..>2014
                     /locus_tag="ACHKAU_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFO5534152.1"
                     /translation="KLFSRNDVETKIIVWYNSTMEKNVFLREVISCVLWTKKVDSYIK
                     ENKKHLSTEDAEALRDYAKLKIKELFSMLDDDGYKRIITTNSYIKERDKLSGIIHNIE
                     NSIISGHESSDIIRSHQHEWGDLSTVEQFKKFEFYVKSELSFSKSIFDDIKTKYITDP
                     ETKRNALYHQLDSDIKERIAFLDISHYAYPGSLLEKLQLSGYVFSDINIIAEYLLSSY
                     GISGHYSHGVVYPAPSDKLFELLRRHTNSNSDWIEKIIPYVYDILSGNVSSFLHPPLS
                     EEQKKILSDIKLEISESVSEQYFMKLTEQKSSVIGIKYSVDFDRYNENLFLSLPINQN
                     LTLPFMYRYFEMLYDIHIGILENKANRDFIYRKFSSLNLDFLINDERVFNLEGLIKKY
                     KYLSLSEIHKTLTNSNSFADISIPLLQTICPSITTIIKKTEYHGHQLTNAMTVASVVK
                     PYDFSNLGAINSIDKSVSDIPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHKST
                     NIEFHPQSLLFDRDSKGKCLGLSLLYLDTGGYGSRYQKLRHNIETASALYQTKYNDNI
                     KLSNSDDFFLRKTQRIITMSNELGNNRLKNSQLEILELKDPVLTEGILYQRRISSLLI
                     TTEYHSLALQQVSSFWRVTDPNFGHCDFHSLAQALTFIKNITSNGNF"
BASE COUNT          758 a          308 c          312 g          636 t
ORIGIN      
        1 aagttatttt cgcgtaatga tgtcgagaca aaaatcattg tttggtacaa ttcaacaatg
       61 gaaaaaaatg tattcctccg agaggttata tcatgtgttc tatggactaa aaaagtagac
      121 tcttatataa aagaaaacaa aaaacactta tctacggaag atgctgaagc tttaagagat
      181 tatgcaaaat taaaaataaa agaactattt tcgatgttgg atgatgatgg ttataaaaga
      241 atcataacaa caaattcata tattaaagag agggataaac tatcaggcat tatacacaac
      301 atcgaaaatt ctattatatc aggtcatgaa tcatctgata taatacgaag ccatcaacat
      361 gagtggggag atttatcaac ggtagagcaa tttaaaaaat ttgaatttta tgttaaaagt
      421 gagttatcat tttccaagtc aatttttgat gatataaaaa ccaaatatat aacagatccc
      481 gaaacaaaac gaaacgcact ttaccatcaa ttagatagtg atatcaaaga gcgaatagcc
      541 tttctcgaca tatcccacta cgcatatcca ggtagtttat tagaaaaatt acagttaagc
      601 ggctacgttt tttctgacat caacataatt gcagaatact tattgtctag ttacggaatt
      661 tctgggcatt attctcacgg cgtggtatac ccagctccat ctgataaatt atttgagtta
      721 ttaagaagac atacaaattc caattctgat tggatagaaa aaataatccc atatgtttat
      781 gatatcttat caggtaatgt gtccagcttc ttgcaccctc ccttatcaga agaacagaaa
      841 aaaatattga gtgatataaa attagaaatt agtgaatctg tatcagaaca gtatttcatg
      901 aagttgacag aacaaaaatc atcagttatt ggcattaaat actctgttga ttttgataga
      961 tacaatgaga atttattttt gtctttacct ataaatcaaa atttaactct acctttcatg
     1021 tatcgctact tcgagatgtt atatgacatt cacattggga tcttagaaaa caaggcaaat
     1081 agagatttta tatataggaa gttttcatct ttaaatttgg attttttaat taatgacgag
     1141 agagttttta atcttgaagg cttaataaaa aaatataaat atttatcatt gagtgaaata
     1201 cataaaactt taacaaatag taattcattt gcagatattt ccataccatt attacagact
     1261 atatgtccca gtataacaac aataataaaa aaaacagaat atcatggaca tcagttaaca
     1321 aatgctatga cagttgcttc agtggttaag ccatatgact ttagtaatct tggtgccata
     1381 aatagtatag ataagtccgt ttctgatata cctgcattgc atacaattgt tgaacaagcg
     1441 aagtataatt tactttcatg gaatgatttt tataatactc atgcgtcaat atgggataca
     1501 atagcaaggc agcataaaag tacaaatatc gaatttcacc cacaaagtct tctttttgat
     1561 agagattcga aaggaaagtg ccttggtttg tctctcctgt atcttgatac aggaggttat
     1621 ggaagcagat atcaaaaact gagacacaat attgaaactg ccagcgcatt gtatcaaaca
     1681 aaatataatg acaatattaa attatctaat agcgatgatt tctttttaag aaaaacacaa
     1741 cgtatcatta caatgtccaa tgagttaggc aataatagat taaaaaactc acagttagaa
     1801 atcctagaat tgaaggaccc tgtactaact gaaggtatat tataccagcg tcgaatatca
     1861 tctcttttaa taacaacaga atatcactca ctcgcgttac aacaagtatc ttctttttgg
     1921 agagtaaccg atcctaattt tgggcattgt gacttccatt ccttagcaca ggctttaacg
     1981 tttataaaaa atataacgag caatggtaac tttt
//
LOCUS       DAZFJF010000324         1996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFJF010000324 DAZFJF010000000
VERSION     DAZFJF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.326"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..>1996
                     /locus_tag="ACHKAU_005297"
     CDS             33..>1996
                     /locus_tag="ACHKAU_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO5534153.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     PSVQEKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVAREEL
                     QALHIARAEELPAVTALAISHKTKLLDPLEIRDLHKLVRDTDKVFPNPGNSDLGLITA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTFDTLD
                     VEIAAATLPMDFNIYEIPGSVYRRAKEIVLKRESPFKEWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTAARHTPEKDILEEVNRELS
                     AKQETEEEENDEEKPQPSCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNSVDAKY
                     QKLRAELHEARKNIPPPNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQN
                     QQETEQNDQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCGAVM
                     GDATYQETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITV
                     NGHHVITSTSRTCDHLMIDLETMGKNPDAP"
BASE COUNT          607 a          499 c          494 g          396 t
ORIGIN      
        1 gttcaccctt aaatctgtaa tgaggtttat caatgagcac tgataaagaa gaatttgcgc
       61 tatattgcga agcaaaaaat gacaaagtca gaaaacgtct gggaattaaa ggtggttttt
      121 actggactac agcaaaaaaa ttatctgttg ccatctcccg ctgcattacc gcaatggatg
      181 acaacgatta tgatgaagac gactttaaaa aacccgttcg cgtccattta cccgttgtga
      241 atgaccttcc acctgaaggc gtgtttgata ccgaattctg caaccgatac gaaaaaggcg
      301 gggaagatgg cattacaatg gtatttatcg cgccctctcc ctccgtgcag gagaaaccag
      361 ccagtactga caataccaac gtcaacggcg aagacatgac ggagattgag gagaatatgc
      421 tcctgccggt ttctggtcag gagctgccca ttcgctggct tgcgcaacac ggcagcgaaa
      481 aaccagtaac gcacgttgca cgggaagaac ttcaggcatt acatatagca cgggcggaag
      541 aactgccggc tgttactgcc ctggccattt ctcacaaaac aaagctgctc gacccgctgg
      601 agattcgcga ccttcacaaa ctggtacgcg acactgacaa agttttccct aatcccggta
      661 attcagacct gggactgata actgcttttt tcgaggcata cctggacgct gactacactg
      721 atcggggtct gctgacaaaa gagtggatga aaggaaatcg tgtttcgcgt atcacccgta
      781 cggcttccgg tgcaaatgcc ggtggcggga acaaaaccga tcgcaatccg aatttagtac
      841 acaccttcga tacgctggat gtggagattg cagcagccac acttccgatg gattttaata
      901 tttatgaaat tccgggcagc gtttatcgtc gcgcaaaaga aatcgtcctg aaaagagaaa
      961 gtccgttcaa agaatggtcc gcagcacttc gcgcaacccc gggtattctg gactattccc
     1021 gcgccgctat ttttgcactt atccggagcg ctcaccctga gttttatcac tacccgggac
     1081 gccttcaggg gtatatcaac gcctacttaa cggagactga tcacgagaac cccagcaagg
     1141 aaactctcac tgctgcacga catacaccgg aaaaagatat cctggaagaa gttaaccgcg
     1201 aactgtctgc taagcaggaa acagaagaag aagaaaatga tgaagaaaaa ccgcaaccat
     1261 cttgcgcaat ggcagatgaa caggcaacgg ctgaaacagt ggaaccggat gcaactgaac
     1321 atcatcagga cacgcagccg ctggatgctc agtcacaggt aaattctgtt gatgcgaaat
     1381 atcagaaact gcgggcagaa ctccatgaag cccggaaaaa cattccgccc ccaaaccctg
     1441 tcgatgcaga caaattactg gctgcctctc gcggagaatt tgttgaaggg attagcgacc
     1501 cgaatgatcc gaaatggatt aaggggatcc agacccgcga ttctgtgtac cagaaccagc
     1561 aagaaacgga acagaacgac cagaaagcgg aacaaaacag cccaaatgcg ttacaaaacg
     1621 agccagaaac gaaacagcct gaaccagtgg cgcaacagga agtggaaaaa gtctgcaccg
     1681 cctgcggtca gaccggcggc ggcaactgcc ctgattgtgg cgcggtgatg ggcgacgcaa
     1741 cataccagga aacattcgat gacaagaacc tggttgaagt tcaggaagac gattcggaga
     1801 aaatggaagg cgctgaacat ccacacaagg agaatgctgg cagcgctcag gaccacgcca
     1861 gcgatagtga aactggcgag acggcagatc ccttaattac ggtgaacggt catcacgtta
     1921 tcacatccac cagcaggacg tgtgaccatc taatgatcga ccttgaaacc atgggaaaaa
     1981 atcctgatgc cccgat
//
LOCUS       DAZFJF010000325         1966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFJF010000325 DAZFJF010000000
VERSION     DAZFJF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.327"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(608..790)
                     /locus_tag="ACHKAU_005298"
     CDS             complement(608..790)
                     /locus_tag="ACHKAU_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5534154.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(787..1314)
                     /locus_tag="ACHKAU_005299"
     CDS             complement(787..1314)
                     /locus_tag="ACHKAU_005299"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5534155.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(1311..1757)
                     /locus_tag="ACHKAU_005300"
     CDS             complement(1311..1757)
                     /locus_tag="ACHKAU_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5534156.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            complement(1765..>1966)
                     /locus_tag="ACHKAU_005301"
     CDS             complement(1765..>1966)
                     /locus_tag="ACHKAU_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534157.1"
                     /translation="ELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFGSI
                     FLWVIIGVAPVAVAKMAWRFVS"
BASE COUNT          531 a          507 c          384 g          544 t
ORIGIN      
        1 atgaaaaaga tatttcgcgt ttaagcatgg gtaacgaagt taatgaaaaa ataattgcag
       61 ttatagtcaa gacgttagca tatagagagt ctatgttgtg ttagacttta actctatatt
      121 gatataacat tagccaaata ttcaacagtg tatgcgacca aacaccaaca tgtcgcatac
      181 atatacaatt tgaatattta attatattta cttaatgtat ttctataaaa gcaaattaca
      241 aactcacaac aaaaaacctc ataacacatt aacaatcaat tcttttcatc tttataaatt
      301 ctcatatcag gcttgcaccc gataaaccga cgaaaactat ttaaaaccca tcgagtgaag
      361 taatctctaa aaccaaagaa ataccaagtg aaaatattca cgataaaatg cccggtcaaa
      421 gcccctcctg taccgcatgc aagaacagta aaaaatcaga tgttttcata aatatcagtc
      481 ctcatcgttt tgcctggcat gtcctttacc agcaatcttc tgtatgcact aagcctagat
      541 agaatccact cagtgtacac tgaagcccgc tcgacgcttt cttgttcgta acttcgattt
      601 tagtcaatta ccttgttttc ctcgcacgat gtcttagcca ccggatatcc cacaggtgag
      661 ccgtgtaatt gaaggttttt acgtcagatt cttttgggat tggcttgcgt ttatttctgt
      721 agcgtttcgt tggaaggtat ttgcagtttt cgcagatgat gtcggtgaaa cttcgtcgct
      781 gtcgcctcat gccgccctcc tgacgccctg cccgatcgcc atcaatgccg ctttggatac
      841 ggtagtaaac atccgtcgag gactgatgaa cggtcgccaa atcagcagca tggagccttt
      901 actgtttccc ttcttctcca gccctgtcga tggttcgata aaattaatcc gtccatcagt
      961 gataatgcga acttcgtcaa cactctccag agccttgctg aaccatccga cagacatatc
     1021 ctctggcaca agcatcacta ccgtctgtcg ctgttgtatg cactgctcag cggctttttc
     1081 cacccacggc ctgatattgc tgtacggtgg gttattccag attgcaccgt ggcttatcca
     1141 ctcagaattg agtgcgtcgt cggcctcagt tagccagtga gcgcacagag catttttgtc
     1201 gctcgcagct gaatccagcc agaatccaaa ctcaatatcc agcgcatcaa aaagccaaag
     1261 cggcgtttgc cagcagtcct tgtcgtgtgc tggtgtattt gatttgatag tcatgcagcc
     1321 ctaccttttc gttgtgacca ttcatactct cgccgggagt catcactcca ccgcacgttg
     1381 cgctctgagc cgaaccaaaa catgatttcg ataagctcag tcatgctggc ctttcgcatt
     1441 ttgctggtac gcacgccaag catgacaacg ccaccatcga taccaggcac acttcgttgc
     1501 tccagttttt tggtcttaag ccacagggca gtgaacaggt ctttccagtc ctccggcgca
     1561 agtctctgtc catgccaaag cacctgacgt gatacgtcct gcaataacgc ccacataagg
     1621 cggttttgag gattgctccg ctttggttct ttaatgtgga cttcgtgagg tgacttgtcg
     1681 tcgatcggaa gtgagagtat tgcgtctatg gcgttgtttc tgattgcttc gttgcgaagc
     1741 atgtatattt gcttcattgt cacctcaact cacaaaacgc cacgccattt ttgctacagc
     1801 gacaggcgca acaccgataa tcacccacag gaaaatgcta ccgaaaagca caccaaccag
     1861 gtctttacct tcgcctacca gccggacaaa actgctggca accacaatga acgtcgccac
     1921 catccacata gcaccgagaa tcctcaatgc agaaaaaatc aactca
//
LOCUS       DAZFJF010000326         1965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFJF010000326 DAZFJF010000000
VERSION     DAZFJF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.328"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..397)
                     /locus_tag="ACHKAU_005302"
     CDS             complement(77..397)
                     /locus_tag="ACHKAU_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5534158.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            505..678
                     /locus_tag="ACHKAU_005303"
     CDS             505..678
                     /locus_tag="ACHKAU_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5534159.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            749..1672
                     /locus_tag="ACHKAU_005304"
     CDS             749..1672
                     /locus_tag="ACHKAU_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO5534160.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLSKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKKTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSCRCYDYGHEFSYVIGMARDVLINHTR
                     DIDINEPDGPTNLSAWLRLKNKELPPSLHHY"
     gene            1727..>1965
                     /locus_tag="ACHKAU_005305"
     CDS             1727..>1965
                     /locus_tag="ACHKAU_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5534161.1"
                     /translation="MLYSNILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADR"
BASE COUNT          525 a          435 c          467 g          538 t
ORIGIN      
        1 atgcgcggca tggtggctaa ttttggcggt ttcctttcca tcaataaact ttcgcagtaa
       61 tggattatgc ccaccgtcag gtgggttttt tatttagtag ttctctcaac ttttcgttct
      121 gctctctgaa cttttcctgt atttcttttt gcatggcgat aacctgggct tgaagttgaa
      181 ctagcgcatc aacatttagc ggaaccgaga cgctgttaat tttatctgct atttccccga
      241 gtgtattttc tgcattcaag gcatcttcca gtatctgaac aatctctgag ttcatagagc
      301 gcccgtttcg tttggctcgt tcagctatag catctcgcat tccgtcagga aaacggaggt
      361 tgaacttgtc gtagtctttt acttgttttt cggccattgc aaatctctca aaaaaaatca
      421 tggtgccata ttgccatacg atttcaatgg tggcattatg gctctcatgg tgtcactttg
      481 gcaccaaata aaggagatta gataatgcaa gatacacttt tcactgagcg caaaaatatc
      541 aaactcaacc ttcgccttcc atcacggctg aatgaagacc ttcgccgcct ggcggaaatg
      601 gactgtatat ctctgaactc tgcaattgtt cgtttgctgg caaaaggtgt tagggaagag
      661 gtggcgaatg gtcgctaaaa acagcgaagc ctcaatggct gcaaccattg aggcttctaa
      721 attaccagtt aaccacgaga aaactgatat gactagttta gcaattgcag atcgcacaat
      781 caatgttcca ttccacggaa caaatctctt tttggttgga attaacaatg agccttatgt
      841 tcctatgaag cctgttgttg aaggtatggg gatggtttgg gctgctcaat ttgttaagtt
      901 aaaacagagg tttgtcaaag gtatttcgga aatcgaaata ccttctgctg gcggtaaaca
      961 gttaatgaca tgtcttgcct ttcgtaaatt tgcggcttgg ctttcaagca ttcaaccaaa
     1021 caaagtccgc cctgaaatcc gcgacaaggt aatccagtat caggaagagt gtgacgatgt
     1081 gctctacgag tactggacta aaggccatgt gattaacccg cgcaaagcta aaaaggcatt
     1141 gccggggaaa atcaccactg aacagcagga agccattaaa caactcgtca tgagtcgcgg
     1201 tcagtctctg tcaaaagaaa aacaggcgaa ggcgatgatc accatgtggt cgtcactgaa
     1261 atctcatttt gggtgttcat acaaagaaat cagcgaggag cagtttaccg aagctctgtc
     1321 acttgctgct cgcgttccgc ttgaaggaga gttaatcggc aaacaagaga agaaaaccaa
     1381 cgagctttct gcaaaagaag caaacagcct tgtatggtta tgggattatg ctaaccgctc
     1441 acaggcatta ttccgcgaac tgtatccggc gctaaagcaa attcaatcga actattcctg
     1501 cagatgctac gactacggtc atgagttctc gtatgttatt ggaatggcga gagacgtttt
     1561 aatcaatcac acgcgagata ttgatattaa tgagccagac ggaccaacga atctttctgc
     1621 atggctaaga cttaagaaca aagaattacc tccttcactg catcactact aacagattgc
     1681 caacgaaatg acccagcttc ggctgggttt tttatcagga gttctcatgc tctatagcaa
     1741 tatattggcg cacgcccggc ggtgtgcgcc tgcggagtcg tgcggcttcg tgataagcac
     1801 gccggagggg gaacggtata tcccttgtgt gaatatctct gcagagccgg aggcgtattt
     1861 tcgtattgca ccggaagact ggctgcgggc agagatgcag ggggagattg tggcactggt
     1921 ccacagtcat cccggtggtc tgccctggct gagcgaggcc gaccg
//
LOCUS       DAZFJF010000327         1951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFJF010000327 DAZFJF010000000
VERSION     DAZFJF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.329"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..768)
                     /gene="nleH1-2"
                     /locus_tag="ACHKAU_005306"
     CDS             complement(<1..768)
                     /gene="nleH1-2"
                     /locus_tag="ACHKAU_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO5534162.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGES
                     LLDIPSLPAQAEQAIYDMFDRLEKKGILFVDTTETNVL"
     gene            complement(875..946)
                     /locus_tag="ACHKAU_005307"
                     /pseudo
     CDS             complement(875..946)
                     /locus_tag="ACHKAU_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            940..1934
                     /locus_tag="ACHKAU_005308"
                     /pseudo
     CDS             940..1934
                     /locus_tag="ACHKAU_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          585 a          463 c          401 g          502 t
ORIGIN      
        1 taaaacattt gtttctgttg tatcaacaaa aagaattcct tttttctcca gtctgtcaaa
       61 catatcgtaa atagcctgtt cagcttgtgc tggtaatgat ggaatatcca aaagagattc
      121 cccatttatt ttattcattc taataccaat aacatttcca ttatcattat atattttctc
      181 tgcactcccg gaaccataat actgattgaa acaacgaact tcgcttgtca cctcttcatg
      241 gctttgagat atagtaaaca tcttcaacac ttttgttgta tcttccatat cttcatacac
      301 gacagcatta ccacctttac caataacatt acctggcacg ggtgatttat tgttgctaac
      361 cctcggcaac tctgattgag tataatccgc tggaggtaac tccggctgcg cataatccac
      421 tggaggtaaa tcaggtctgt tcgaatgaac agagcctctt tctatagtcg tgctcactgg
      481 cgatgtattc agcatagcct caatttttct gctaatctct cccttaggcc atcccaacct
      541 gtgcaacaga ttagtaaaac aaccactatg actttctctt gtaacgcaaa acttattatc
      601 agtgaccaca acacgatatg ttctgttgcc aaccgttact tgcgtcccgc tatcagagtg
      661 aacagcagca tcccttacag aggataaaac acgattatca ggcgaagtca ggtttctggt
      721 taaagaattc catgaacatc ccaaatttat agaagagggc gataacatac atttcaacct
      781 tcaaaataaa cctatctaat tattcctaac agacatcccc catacatgac aacaaaaacc
      841 ggagccggac tccggttttt gtgaagctat cgggttactt catttcgcca atattttccc
      901 acttcccgtc agcacgcagg atttgcagcg gtcttactac acactgaccc gaggacgaaa
      961 aaagagttcg ggctgggaag agacagaaga gtagcaattt cagaagcatt cacaccaaca
     1021 ttgagctttt cagcgaaagt gggcacgaat cattgctgga cagggttaaa ggcactgact
     1081 caatcactct tcatacgtgg cttgatcgat atgaaacaat cctcagctag agggggagca
     1141 agccgaaaac attactcgac tacgccagca aaatcagggc aattcgaaga aaattgccgg
     1201 acaaaccgct cactgacata tcaacgaaag aggtggcagc aatgctaaac acctacgtcg
     1261 cagaaggtaa agcggtttcc gcaagagtaa tcaggtcaac ccttgttgac gtttttcgag
     1321 gggcaatagc cgaggggcat gtggcaacga atccagtaac aacaacccgt gcagcaaagt
     1381 cagaagtaag gcgctcaagg ctgacagcta atgagtatgt cgctatttac catgctgctg
     1441 agcacctccc catctggctg aggctgtcaa tggatttagc tgtcgttaca gggcagagag
     1501 tgggcgattt gtgcagaatg aagtggtcag acataaacga tggtcatctt cacattggac
     1561 agagtaaaac aggagccaaa attgccattc cgctggctct aaccattgac gcactcgaca
     1621 tctcactggt tgatacacta cagaaatgca gggaggccag cagcagtgaa acaataatcg
     1681 catcaaccta tcacgaacca ctttctccag ccacagtatc acggtattta acaaaggcgc
     1741 gaaatgcatc cgggatctcg tttgatggag acccaccgac atttcatgaa ctacgtagtc
     1801 tgtccgcgag gctatatcgg aaccagattg gctacaagtt tgcacaacgt cttcttggac
     1861 ataaatctga ttcaatggcg gcgcattata gggacagtcg cgggcgggag tgggacaaaa
     1921 ttgaaatcgg ataatgattt tattttgacc a
//
LOCUS       DAZFJF010000328         1894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFJF010000328 DAZFJF010000000
VERSION     DAZFJF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.330"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            305..>1894
                     /locus_tag="ACHKAU_005309"
     CDS             305..>1894
                     /locus_tag="ACHKAU_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534163.1"
                     /translation="MIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAASTNVYP
                     ITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDVVINI
                     PQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEIIPKENESAE
                     TVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLIQALF
                     FNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLFHAPT
                     IFYQYPKLIDEVKYCISNKKITGSVIARFTLCLTSTLLTMSPLLMLNGAVKTGSIVRT
                     IGRGVSYVDIPLALAILGDSWYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLS
                     LMSSLSGAIMRSLEKGTPPQMMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNP
                     DILNTQAIALCVDLQHTKNITMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQ
                     GERAILNTSQSETCKHKIDEIY"
BASE COUNT          689 a          364 c          299 g          542 t
ORIGIN      
        1 caccacattc ctgatgtcat actcactaaa caatttttcg acaacatggt agctcataga
       61 acgttatcgt gtagacaccc tctgctacgt attcggtgtt cagtgcaata tctacaaata
      121 ctggaaaaat ctaaaattga ggcaagtttt tggtgataac tatagttaga ctatattgac
      181 gacctgatgt gctgtatgta ataactaaca aaaaatattt tccatgggat tttttatttt
      241 aatgaaatgc aaaatatttt tatcaatggt tagtattatg gaaaccatta attcaggagg
      301 aaacttgatt ccaaattcaa cttcaaataa aggttatgta tgcattgaca tgcagtgttc
      361 gtcaacctct gaaccaacag cttctacctc cagcaaccgg agtattaaat tagccgcttc
      421 cacaaatgta tatccgataa caagaaacga ctccgagctc actctgaacg attttcttga
      481 taatagctct tctacgtcat cattggacta cattaatgaa ttgggttctc aactgacgtt
      541 aaatgatttt cttgacaaca taaagacaaa tgaggtggat agaacatgta cggatgtggt
      601 aattaatatc ccacaagaga tacaaacaaa tacacaggaa aatgatttgt tattatccga
      661 taaaaataat tcaatatgca ttgaaatcga tgaaagaatt acaaaaatcc taacatgcaa
      721 gcaaaaatat caactggaca gcatcattca tgaaattata ccaaaagaga atgagagtgc
      781 agaaactgtt ctccatctta tgagagttct gaacgatcaa tatcatcagg tatataatca
      841 gtcaggatgt ttttataaag cctatatggc catacacaat aaaatcgaac agatacttcc
      901 atatgcgttc agagccggag gcggaatcag cattcacttg ctcatacagg cattattttt
      961 taatggcgac tataacaaat caccttcaca gtctcaacaa ccatctttat atacatcacc
     1021 ttctccaaca ataaatacag aagcattctt aagtaatgta ttatcactag atataaccca
     1081 ggtacgcata cttggtgatt tactatcagc aactttattt catgcaccaa caatattcta
     1141 tcaatatcct aaactaatag atgaagttaa gtattgtata agtaataaaa aaataacagg
     1201 ttcggttata gcacgattta ctctatgttt aacaagtaca ttactaacca tgtcaccact
     1261 gttaatgctt aatggagcag ttaaaacagg tagcatagta agaactatag gtaggggagt
     1321 gagttatgtt gatataccat tggccttagc tatattaggt gactcgtggt ataaagctta
     1381 taaacatggt tcttctgata acccaaattc tgctcagaga tttatatcgc aagaagcggc
     1441 ctttaaaacc acccagcggg tattaacaca aggattaagt ctgatgtcct ctttatcggg
     1501 agcaatcatg cgctctcttg agaaaggcac accaccacaa atgatgtctt tattcatcgt
     1561 aaacatacta aatctattat ttcatcaaaa tccatatgaa ggggcatcag caagtgctaa
     1621 tgctttgaaa agatcaactt actcccataa tccggacata ctaaatactc aggcaatagc
     1681 tctttgtgtt gacctccagc atacaaaaaa tataaccatg ccacttttca aaacaaaaga
     1741 caggatatct tacgcattca atggacaaag aacatcccca gaagaccaaa aacaaatact
     1801 gaaagaagtt ataaactcct gtacccaagg agaaagagcc attttaaata catcacaatc
     1861 agaaacatgc aaacataaaa tcgatgagat ttat
//
LOCUS       DAZFJF010000329         1889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFJF010000329 DAZFJF010000000
VERSION     DAZFJF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.331"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1536
                     /locus_tag="ACHKAU_005310"
     CDS             <1..1536
                     /locus_tag="ACHKAU_005310"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5534164.1"
                     /translation="YSSDLSDEHLLSSHIIYSNIQKVTSSRGNPLISRVPPDFFDLII
                     IDEAHHAPAQSWRDTLHYFSNAKILHVTGTPYRGDNQEIPGVKIHETPLSEVMRDKYV
                     KWLRKETVNAHDLFFYTPEQPGVKLSKEQVLMFKDKEWIEKSIALSKECSLDVINHSI
                     KKLNELKELSPNVPHKILAIGCSISHAEDLLSWYKAKGMTSVIVHSEMDQNDLSDALM
                     KIEHHQCDVVISVNMLMEGYDHKYLTVLAIFRPYRSLNAFAQVVGRILRAIPESEITH
                     FEIDNNGIVIFHEEIGLNVMWETFQKEVERARRASVREYTFTDRDYHERSNTLAGIES
                     DSAYVSDTDSYLDDVDFNAMFEQKRNEINNSVEQQIEKIKSSGVDLPEDALEALRQTL
                     AEKAVQKAAEIIDPELIEKRPHQARKLMRNILKTRTQDGVSNLLIDNGIGEKSSELYS
                     IFNRHVPYLKTTDPNDGILVRYVNLKLAKAYGPVDKRDNKTLLQSINDLDKILAEVER
                     MIK"
     gene            1533..>1889
                     /locus_tag="ACHKAU_005311"
     CDS             1533..>1889
                     /locus_tag="ACHKAU_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534165.1"
                     /translation="MTNYSQIMEEINKIISFCMVKGVQPHELISAIFEDEYKHIETYK
                     KGEHIHLILSYSDTHEDGVNNIKMRYIYNNKHQLLSVAQKIDASSYKTQWDRSEKLDE
                     MLNKLALKLPKDSLVIN"
BASE COUNT          701 a          301 c          341 g          546 t
ORIGIN      
        1 tactcatctg atttatctga tgaacatctt ttaagcagcc acataattta ttcaaacata
       61 caaaaagtaa catcttctcg aggcaaccca ctaataagtc gtgtaccccc tgactttttc
      121 gatctcatta ttattgatga ggctcatcat gcccccgcac aaagttggcg ggatactctt
      181 cattattttt ccaatgctaa gatattgcat gttactggca ctccatatag aggcgacaat
      241 caagaaatac caggagtaaa aatacatgaa actcctttat ctgaagttat gcgggataaa
      301 tatgtcaaat ggttaagaaa agaaaccgtc aatgcacatg atttattctt ttacacgcca
      361 gaacagccag gagtaaagtt atctaaagaa caagttttga tgtttaaaga taaagaatgg
      421 atagagaaaa gtatcgctct ttccaaagag tgctctttag atgttattaa tcatagtata
      481 aaaaaactta atgaactaaa agagctgtca ccaaatgtcc cacataaaat tttggctata
      541 ggctgtagta tatctcatgc tgaagattta ctttcatggt ataaagccaa aggaatgaca
      601 tcagtaattg ttcatagtga aatggatcaa aacgaccttt ctgatgctct aatgaaaata
      661 gaacatcatc aatgtgacgt cgttatatct gtcaacatgc tcatggaagg gtatgatcat
      721 aaatatctaa cagtacttgc tatatttaga ccataccgta gcctaaacgc ttttgcacaa
      781 gttgttggac gcattttacg agcgattcct gaatcagaaa ttacccactt tgaaatagat
      841 aacaatggaa tagtcatatt ccatgaggaa ataggtttaa atgtcatgtg ggaaacattt
      901 cagaaagaag tggagagagc acgcagagca agtgtaagag aatatacatt tacagacagg
      961 gattatcatg aaagatctaa tacattagct gggatagaaa gtgatagtgc ttacgtaagt
     1021 gatacagact cttacctaga tgatgttgac tttaatgcta tgtttgaaca gaagcgtaat
     1081 gagatcaaca attctgttga gcaacaaata gaaaaaataa aatcgtctgg agtagatctt
     1141 cctgaagatg ctcttgaagc attacgccaa acattagcag aaaaagctgt gcaaaaagcc
     1201 gcagagataa ttgatcctga gctaattgaa aaaaggcctc atcaagcgag aaagttaatg
     1261 agaaacatct tgaaaacccg cactcaggat ggtgtttcta atttattgat tgataatgga
     1321 attggtgaaa aaagctccga actgtatagt atttttaacc gacatgtacc ttatttaaag
     1381 acaactgatc caaatgatgg tatattggtt agatatgtta acctaaaatt agccaaagca
     1441 tatggtcctg ttgataagcg ggataataaa acccttcttc aatcaataaa tgatttggac
     1501 aagattctcg cagaagtaga gaggatgatt aaatgacaaa ctattctcaa attatggaag
     1561 aaattaataa aataatttct ttctgtatgg ttaaaggtgt tcaacctcat gaattaatta
     1621 gtgcaatatt cgaagatgaa tataagcaca ttgaaactta taaaaaaggt gagcatatac
     1681 atcttatatt gtcttattca gacactcatg aagatggtgt caacaatatt aaaatgagat
     1741 atatttataa caataagcat cagctattat ccgttgcaca aaaaatagat gcatcaagtt
     1801 ataaaacgca atgggaccgt agtgaaaaac ttgatgaaat gctaaataaa ttagccttaa
     1861 aattacctaa agatagcttg gtaattaat
//
LOCUS       DAZFJF010000330         1865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFJF010000330 DAZFJF010000000
VERSION     DAZFJF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.332"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..429
                     /locus_tag="ACHKAU_005312"
     CDS             127..429
                     /locus_tag="ACHKAU_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFO5534166.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMTPENGVSRNIRI
                     AAGIALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
     gene            513..890
                     /gene="sctI"
                     /locus_tag="ACHKAU_005313"
     CDS             513..890
                     /gene="sctI"
                     /locus_tag="ACHKAU_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO5534167.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            892..1464
                     /gene="escJ"
                     /locus_tag="ACHKAU_005314"
     CDS             892..1464
                     /gene="escJ"
                     /locus_tag="ACHKAU_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO5534168.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1470..>1865
                     /gene="sepD"
                     /locus_tag="ACHKAU_005315"
     CDS             1470..>1865
                     /gene="sepD"
                     /locus_tag="ACHKAU_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO5534169.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQAS"
BASE COUNT          643 a          339 c          351 g          532 t
ORIGIN      
        1 ataatatata tagtatatgt actttatgta cattgcaact atttaaataa tataatagtt
       61 gcccatggga taatttggtt attgataacc agacaaatgt ctaaattaga caatggagaa
      121 aagttaatgg atgcagcaaa tttaagtcct tctggcggag tattaccact ggcagccaca
      181 atcaatggaa atgtttcggt ggatgaaaat acaggagtaa tgacaccaga aaatggggta
      241 agtcgtaata tcagaattgc agccggaatt gcactcgcta ctacagctct ggctgcttta
      301 ggtactggta tcgcaatggc atgcacaaca gatgcaagct cccaagaatt cttaggttta
      361 ggtattgcta ccggtgtatt aggtggtgtt actgcgcttg gcggcggatt agcgatgaaa
      421 tatgcctaat catcgcatcg cataaataaa atagagaggt catggatgca ttatgccatt
      481 acctctctca tgaaaagaga ctaaccgtaa atatgaacaa tattaatcaa tcagaaaata
      541 ttaatattca ttttaataaa gtatcccaaa caaatttcgt tgatgaccat accccgttag
      601 caccaacgcc ctctgcggct ggagcggctc agtttcttga tcaattactt cctaaaacag
      661 ctggtgtatc ttctccagag caagttttaa ttgaagaaat taaaaagaga catcttgcaa
      721 caatgaacac cgatctcagt ttcgatgccc tatctgcagg tggactctcg ccagaagatg
      781 tgctcacatt acaaaagaat gtgctcaacg caaacgtcaa tgttgatgtc gtatccaagt
      841 tagcaagtct tctttcaaca tcggttacaa aactggtttc aatgcaataa aatgagaaaa
      901 tacattaaaa acaccttttt atttgttttt atttgtttga cagttgcatg taaagagcaa
      961 ttgtatacag gattaacgga aaaagaagcc aatcagatgc aagcattgtt gttatccaat
     1021 gatgtcaatg tatctaaaga aatggacaaa agtggaaatg taacaataag tgtggaaaaa
     1081 gaggattttg tccgtgcgat aaccatttta aataacaatg gcttcccgaa aaagaaattt
     1141 gcagatattg aagttatttt tccgcccagt cagctagtgg catcgccatc acaagaaaat
     1201 gcaaaaatca actatctcaa agaacaagat atagaaaggt tactaagtaa aatccccggt
     1261 gttatcgatt gcagcgtttc attaaatgtt aacaataacg agagccaacc atcctcagcg
     1321 gctgttttag tcattagctc gccagaggtt aacttagcac catcagtcat tcagataaaa
     1381 aacttagtta agaatagcgt agacgatctc aaactagaaa atattagtgt cgttatcaag
     1441 tcatcctcag gacaggacgg gtaatatgta tgaacaataa taatggcatt gttaagaatg
     1501 atagtgactg gctaacggct cttgattttg taaaagatgt caaaggttca ccaaagcatc
     1561 ttacttttca tatctatcaa aaaaatgcct tcttacacga ctttggaagt tactgggttc
     1621 tgtatatcga gcttaatggc gattttcgcc aggttccaac agataccttc atcagactct
     1681 gtaatattct ggccgttagt aaagaatata aacaaatggg catttttcaa tctaataaaa
     1741 aatggtatct ttgccaactg tttaataaag ataataatca tcgcgcaaac atgtcaaaag
     1801 caataatgca acacacactc gcctctttgc ttgacaagca atttgataag ttagagcaag
     1861 catct
//
LOCUS       DAZFJF010000331         1834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFJF010000331 DAZFJF010000000
VERSION     DAZFJF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.333"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            222..>1834
                     /gene="tir"
                     /locus_tag="ACHKAU_005316"
     CDS             222..>1834
                     /gene="tir"
                     /locus_tag="ACHKAU_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO5534170.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLT
                     KEAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          509 a          401 c          458 g          466 t
ORIGIN      
        1 cacgtggcat ttatttttat atcgcatcat tattttcatt taattacact gatgtgtatt
       61 tttctgcatt attttcttgc atcaaaaatc agactgtgat ttatttggtc gatgttatgt
      121 tcagccgtat atcgactacg tactgtatac cattacatta ttatgtaata gcaatataaa
      181 ggattattat ctatttacat aaaataaaaa ggagatattt tatgcctatt ggtaatcttg
      241 gccacaatcc caatgtgaga gctttaattc cacctgcacc gccattacct tcacaaaccg
      301 acggtgcagg aggtgcccgt aatcagctca ttaactcaaa tggcccgatg gggtctcgtt
      361 tgctatttac gcctataagg aattctgttg ctgatgctgc tgattctcgt gccagtgata
      421 ttcccggact tcctacaaat ccactgcgct ttgctgcgtc cgaggtatct ttgcatggtg
      481 cgcttgaagt tcttcatgat aaaggggggc ttgatactct taactctgct attggatctt
      541 cgttattccg tgttgaaact cgggatgatg gcagccatgt tgctatcggg caaaaaaatg
      601 gcctcgagac cactgttgtt ttaagtgagc aagagttttc tagcttacag tcccttgatc
      661 ctgaaggtaa aaacaaattt gtatttactg gaggccgcgg tggcgcaggg catgctatgg
      721 tcacggttgc ttcagatatc gccgaagccc gtcagaggat aatagataaa ttagaaccaa
      781 aggatacaaa ggagacgaag gagccagggg atccaaatag tggcgaggga aaaatcattg
      841 aaattcatac ctcaacctca acttctagcc tccgtgcaga tcctaaactt tggttgtcat
      901 tggggactat tgctgcaggt ctgataggga tggctgcgac ggggattgca caggctgttg
      961 cgttgactcc agagccggat gacccaatca ctaccgaccc tgatgctgca gcaaacacag
     1021 ctgaagcagc ggcaaaagat cagttaacga aagaagcatt ccagaaccca gataaccaga
     1081 aagttaatat cgatgagaac ggaaatgcaa ttccgtccgg ggaactaaaa gatgatgttg
     1141 ttgcgcaaat agcagaacaa gctaaagcgg cgggtgaaca ggccagacag gaagctattg
     1201 aaagtaattc tcaggcgcag caaaaatatg atgaacagca tgctaaacgc gaacaggaaa
     1261 tgtctctttc atcgggggtt ggctacggta ttagtggtgc gctgattctt ggcgggggaa
     1321 ttggtgccgg tgttactgct gctcttcatc ggaaaaacca accggcagaa caaacaatca
     1381 ctacacgtac ggtagtcgat aatcagccta cgaataacgc atctgcgcag ggcaatactg
     1441 acacaagtgg gccagaagag tccccggcga gcagacgtaa ttcgaatgcc agcctcgcat
     1501 cgaacgggtc tgacacctcc agcacgggca cggtagagaa tccgtatgct gacgttggaa
     1561 tgcccagaaa tgattcactg gctcgcattt cagaggaacc tatttatgat gaggtcgctg
     1621 cagatcctaa ttatagcgtc attcaacatt tttcagggaa cagcccagtt accggaaggt
     1681 tagtgggaac cccagggcaa ggtatccaaa gtacttatgc gcttctggca agcagcggcg
     1741 gattgcgttt aggtatggga ggattaacgg gggggggcga gagcgcagta agtactgcca
     1801 atgccgcacc aacgccggga cccgcacgtt tcgt
//
LOCUS       DAZFJF010000332         1804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFJF010000332 DAZFJF010000000
VERSION     DAZFJF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.334"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..96
                     /locus_tag="ACHKAU_005317"
     CDS             <1..96
                     /locus_tag="ACHKAU_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534171.1"
                     /translation="TGDPAREFTMIQSAPLMLLADPDEFVSVQLA"
     gene            138..533
                     /locus_tag="ACHKAU_005318"
     CDS             138..533
                     /locus_tag="ACHKAU_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5534172.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDTAGQDTPLSQENVLTGHENEVVSAQTDTVTDTAALVTVVALVTLHTVALHATRDEP
                     VAFVLPGTAFRVSASVAAEMTEHGLARMQ"
     gene            545..898
                     /locus_tag="ACHKAU_005319"
     CDS             545..898
                     /locus_tag="ACHKAU_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5534173.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGMSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            913..1446
                     /locus_tag="ACHKAU_005320"
     CDS             913..1446
                     /locus_tag="ACHKAU_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534174.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            1443..>1804
                     /locus_tag="ACHKAU_005321"
     CDS             1443..>1804
                     /locus_tag="ACHKAU_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5534175.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSA"
BASE COUNT          419 a          439 c          554 g          392 t
ORIGIN      
        1 accggcgatc cggcgcgtga gttcaccatg attcagtcag caccgctgat gctgctggct
       61 gaccctgatg agttcgtgtc cgtacaactg gcgtaatcat ggcccttcgg ggccattgtc
      121 tctctgtgga gaagtccatg acgaaagatg aactgattgc ccgtctccgc tcgctgggtg
      181 aacaactgaa ccgtgatgtc agcctgacgg ggacgaaaga agaactggcg ctccgtgtgg
      241 cagagctgga agaagagctt gatgacacgg atgacactgc cggtcaggac acccctctca
      301 gccaggaaaa tgtgctgacc gggcatgaaa atgaggtggt atcagcacag acggataccg
      361 tgactgatac ggctgctctg gtcacggtcg tggcactggt gacgctgcac actgttgcac
      421 ttcacgccac gcgggatgaa ccagtggcat tcgtactgcc gggaacggcg ttccgtgtct
      481 ctgccagtgt ggcagccgaa atgacagaac atggcctggc cagaatgcaa taacgggagg
      541 cgctgtggct gatttcgata acctgttcga tgctgccatt gcccgcgccg atgaaacgat
      601 acgcgggtac atgggaatgt cagccaccat gacatccggt gagcagtcag gtgctgtgat
      661 acgtggtgtt tttgatgacc ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga
      721 aggctccagc ccgtccctgt ttgtccggac tgatgatgtg cggcaactgc ggcgtggtga
      781 cacgctgacc attaacggcg agatgttctg ggtggaccgt gtttctccgg atgacggagg
      841 gagttgttat ctctggctca accgtgggca accaccggca gttaaccggc gacgataaac
      901 gcagggtgaa ttatggcgat aaaagggctt gatcaggcga ttgaaaatct gagccgggtt
      961 cgtaaaaacg ccattccggc ggcttcagca atggccatta accgcgtggc cacaacggcg
     1021 attaatcagt cttcatcaca ggttgcccgt gagacaaagg tacgccggaa actggtaaag
     1081 gaacggtcca gactgaaacg ggccacggtc agaaatccga atgccagaat tatcgttaaa
     1141 cgcggtgatc tccctgtgat taagctgggg atcaggatgc tgggccgtcg tccggacagc
     1201 atacttaaag ctggtcagca tcggtatcag cgggcattta tccagcgatt aaaaaatggt
     1261 cgctggcatg tcatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     1321 atcccgatgg cggccccact gaaacaggcg tttgatgaga atattgaccg tatccggcgt
     1381 gaacgtctgc ccggagaact ggcatacgcg ctgaaacaac aacttaggat tgcgataaaa
     1441 cgatgaaaca tactgatatc cgtgcggcag tgctggatgc actggagctg catgaacacg
     1501 gggcgacgct gtttgatggt cgccccgttg tttttgacga agaggatttt ccggccgtcg
     1561 cggtttatct gacggatgca gagtataccg gtgaagagct ggatgcagat acctggcggg
     1621 ccacactgca tattgaggtg tttttaccgg cacaggtacc ggattcggag cttgatcagt
     1681 ggatggaaag ccggatttat ccggcggtga ctgcgatccc ggcactggca gacctgatta
     1741 cgacgatggt tacgcagggc tatgagtatc gtcgtgatga cgatatggcg ttatggagtt
     1801 ctgc
//
LOCUS       DAZFJF010000333         1787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFJF010000333 DAZFJF010000000
VERSION     DAZFJF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.335"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..783
                     /locus_tag="ACHKAU_005322"
                     /pseudo
     CDS             1..783
                     /locus_tag="ACHKAU_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
     gene            767..973
                     /locus_tag="ACHKAU_005323"
     CDS             767..973
                     /locus_tag="ACHKAU_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5534176.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDDLN
                     RAINDAESVLGTTSRRRRPLGVRL"
     gene            970..>1787
                     /locus_tag="ACHKAU_005324"
     CDS             970..>1787
                     /locus_tag="ACHKAU_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5534177.1"
                     /translation="MKRMPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          400 a          408 c          596 g          383 t
ORIGIN      
        1 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
      361 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccctcgcctg cggatgaagc cacgtcgtat
      481 gccatccgtt ttcctgatga tccggagatt ttttcgcaga cagaggcgca gcaactggtg
      541 gcggaagagc tggtggagaa gtgagaaaaa ggaaagatgc gtctgctgtg ggataacaaa
      601 aagcggcgta acgaagcgct ggactgcctg gtgtatgcct acgcggcatt acgtgtgtcc
      661 gtgcaacgct ggcagcttga tctggctgta ctggcaaaat cccgggaaga agagacgacc
      721 cggccaaccc tgaaagaact ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc
      781 tgaactgcag gcgctgcgtc aggcgcgcct tgatttatta accggtaaac gggtggtgtc
      841 tgtccagaaa gatggacgaa gaattgaata tacggcggcc tctctggatg atcttaaccg
      901 ggcgatcaat gatgcggagt cagtgctggg gacaacaagc cgccgccgtc gtccgctggg
      961 agtgaggtta tgaaacgaat gcctgtcctg attgatgtga acggtgttcc gcttcgggag
     1021 agcctcagct acaacggggg cggtgcagga tttggcgggc aaatggcgga gtggttgcca
     1081 ccggcgcaga gtgccgatgc ggccctgttg cccgcgttgc gtctgggcaa tgcccgtgca
     1141 gatgatctgg tgcgcaataa cgggatagcg gccaatgcgg tggcactgca taaggatcac
     1201 attgtcgggc atatgtttct gatcagctac cgtccgaact ggcgctggct ggggatgcgg
     1261 gagactgcag caaaaagttt tgtcgatgag gtggaggcgg cctggtcgga atacgccgaa
     1321 gggatgtctg gcgagatcga cgtggaaggg aaacgcacgt ttacggaatt tatccgtgaa
     1381 ggtgtgggcg ttcatgcgtt taacggcgaa atctttgtgc agccggtctg ggatacggag
     1441 agcacgcaac tgtttcgtac gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca
     1501 ggacacggta tggggaaccg ttttctgcgg gccggggtgg aggtcgatcg atatggccgt
     1561 gccgttgcgt accatatctg tgaggatgat tttccgttct ctggtagtgg acgatgggaa
     1621 cggatcccgc gtgaactacc caccgggcgt ccggccatgc tgcatatttt cgagccggtg
     1681 gaggacgggc agacccgtgg ggctaatcag ttttacagcg tcatggaacg gctgaagatg
     1741 ctggattccc tgcaggcaac acagcttcag tcggccattg tgaaagc
//
LOCUS       DAZFJF010000334         1787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFJF010000334 DAZFJF010000000
VERSION     DAZFJF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.336"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..783
                     /locus_tag="ACHKAU_005325"
     CDS             <1..783
                     /locus_tag="ACHKAU_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5534178.1"
                     /translation="VRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEGTLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPTPADEATSY
                     AIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALR
                     VSVQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
     gene            767..973
                     /locus_tag="ACHKAU_005326"
     CDS             767..973
                     /locus_tag="ACHKAU_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5534179.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            970..>1787
                     /locus_tag="ACHKAU_005327"
     CDS             970..>1787
                     /locus_tag="ACHKAU_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5534180.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          395 a          418 c          596 g          378 t
ORIGIN      
        1 gtgcgttaca cggctgcggt gccttcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggcac cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtccc gatgaggaag ggacgctgtt acgtgtggat
      181 gctgcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgaccat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgcg tctgtctatg gcaagccggt gatcaccatg
      361 ccgaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat cccacgcctg cggatgaagc cacgtcgtat
      481 gccatccgtt ttcctgatga tccggagatt ttttcgcaga cagaggcgca gcaactggtg
      541 gcggaagagc tggtggagaa gtgggaaaaa ggaaagatgc gtctgctgtg ggataacaaa
      601 aagcggcgta acgaagcgct ggactgcctg gtgtatgcct acgcggcatt acgtgtgtcc
      661 gtgcaacgct ggcagcttga tctggctgta ctggcaaaat cccgggaaga agagacgacc
      721 cggccaaccc tgaaagaact ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc
      781 tgaactgcag gcgctgcgtc aggcgcgcct tgatttatta accggtaaac gggtggtgtc
      841 tgtccagaaa gatggtcgca gaattgaata tacggcggct tctctggatg agcttaaccg
      901 ggcgatcaat gatgcggagt cggtactggg gacaacccgg cgtcgccgtc gtccgctggg
      961 agtgaggtta tgaaacgaac gcctgtcctg attgatgtga acggcgttcc gcttcgtgag
     1021 agtctcagct acaacggggg cggtgcagga tttggcgggc aaatggctga gtggttgcca
     1081 ccggcgcaga gtgccgatgc ggccctgctg cccgcgttgc gtctggggaa tgcccgggca
     1141 gatgatctgg tgcgcaataa cggaatagcg gctaatgcgg tggctctgca taaggatcac
     1201 attgtcgggc atatgtttct gatcagctac cgtccgaact ggcgctggct ggggatgcgg
     1261 gagaccgcag caaaaagctt tgtcgatgag gtggaggcgg cctggtcgga atacgccgaa
     1321 gggatgtctg gcgagatcga cgtggaagga aaacgcacgt tcacggaatt tatccgtgaa
     1381 ggtgtgggcg ttcatgcgtt taacggcgaa atctttgtgc agccggtctg ggatacggaa
     1441 accacgcagt tattccgtac gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca
     1501 ggacacggta tggggaaccg ttttctgcgg gccggggtgg aggtcgatcg atatggccgt
     1561 gccgttgcgt accatatctg tgaggatgat tttccgttct ccgggagtgg acgatgggaa
     1621 cggatcccgc gtgaacttcc caccgggcgt ccggccatgc tgcatatttt cgagccggtg
     1681 gaggacgggc agacccgtgg ggccaaccag ttttacagcg tcatggaacg gctgaagatg
     1741 ctcgattccc tgcaggcaac acagcttcag tcggccatag tgaaggc
//
LOCUS       DAZFJF010000335         1774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFJF010000335 DAZFJF010000000
VERSION     DAZFJF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.337"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..281)
                     /locus_tag="ACHKAU_005328"
     CDS             complement(<1..281)
                     /locus_tag="ACHKAU_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO5534181.1"
                     /translation="MFGIFGKKARRAAVEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQILRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREI"
     gene            complement(471..680)
                     /locus_tag="ACHKAU_005329"
     CDS             complement(471..680)
                     /locus_tag="ACHKAU_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534182.1"
                     /translation="MFVSEVLMEDKDNKGWVKGWAVVRSSPWHLVGVFATEEDAETEA
                     RKMGDKYEVHYGSHRTGSDDFVWGE"
     gene            complement(733..957)
                     /locus_tag="ACHKAU_005330"
     CDS             complement(733..957)
                     /locus_tag="ACHKAU_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534183.1"
                     /translation="MKKLMVTIGPFETEVSFRVVQGESVLVEDVFHGKSTGPYVKEYL
                     IDATDENIEVVYDSVNHPDLIIKAKLKPLY"
     gene            complement(1048..1124)
                     /locus_tag="ACHKAU_005331"
     tRNA            complement(1048..1124)
                     /locus_tag="ACHKAU_005331"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1088..1090),aa:Arg,seq:tct)
     gene            complement(1138..1214)
                     /locus_tag="ACHKAU_005332"
     tRNA            complement(1138..1214)
                     /locus_tag="ACHKAU_005332"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1178..1180),aa:Arg,seq:tcg)
     gene            complement(1222..1297)
                     /locus_tag="ACHKAU_005333"
     tRNA            complement(1222..1297)
                     /locus_tag="ACHKAU_005333"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1262..1264),aa:Ile,seq:cat)
     gene            complement(1338..1436)
                     /locus_tag="ACHKAU_005334"
     CDS             complement(1338..1436)
                     /locus_tag="ACHKAU_005334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO5534184.1"
                     /translation="MALGRRAIGVELESGRFEQTVREVQNVVSQNG"
BASE COUNT          482 a          470 c          356 g          466 t
ORIGIN      
        1 tcaatttcac gcaatgccgc tcgacgacca attttaaagt tcgtatccag ctgaccgata
       61 atggttgcgc taatcgcatt aatttctgac gtaaacgcgg acaacgcagg ctggttacgc
      121 aagatctgct cgatcttcgc tttctctgaa gcctcacatt caccatctgc ataggccacc
      181 agataggcag cattaataac cgcctgtgcc agatcacgtt tctcaaactt tttaatttcc
      241 actgccgctc ggcgggcttt tttaccaaaa ataccaaaca tcgtgacgtt cctttgggtg
      301 ggtgagccaa cgcccgggag cgatctgccc acagagaaag tcacactgac cactccgtaa
      361 gctcaccccc gaaaggctct gtggttgata tgcgccgggc gtggcgcgga tacaaaaaag
      421 gccgccaata gcgacctcag ttacgggatt attctggggt taaacgactg ttactccccc
      481 cagacaaaat catcacttcc tgttcgatgc gagccatagt gaacctcgta cttatctccc
      541 atctttcttg cttccgtttc tgcgtcttcc tctgtcgcaa aaaccccaac aagatgccag
      601 ggcgagcttc ttaccacagc ccaaccttta acccatcctt tgttgtcctt atcttccatt
      661 aacacttcag aaacaaacat atttatctcc ttgtgggtac ccagagatat tttatgattg
      721 ctcccggtca gatcaataaa gtggcttcaa ttttgcctta atgatcaaat cagggtgatt
      781 gacggaatcg tacaccacct caatattttc atccgtggcg tcgataagat attcttttac
      841 ataaggacct gttgattttc catgaaatac atcttcaaca agtacactct ccccctgaac
      901 aacacgaaaa ctaacttctg tttcgaacgg accaatcgtc accatcagtt ttttcacata
      961 gcctcctgat aagcactcga tttattagtt aatggtgtaa cgcagataca aaaaaaggcc
     1021 cgcaaaagcg agccaagtaa ataaatatgg cgcgttgtac tggattcgaa ccagtgaccg
     1081 attgcttaga aggcaattgc tctgtccgac tgagctaaca acgcatgatg ctgataatgg
     1141 accgccatcg ggaacttgaa ccccgcacag ccagcttcga aggctgacgc tctatcccga
     1201 tgagctaatg gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat
     1261 tatgagtcgc tcgctctcac cactgagcta aagggccggg cgcaggataa taacggtacg
     1321 taactaattc tgcaatatca tccgttctga ctgactacat tctgaacttc cctgaccgtc
     1381 tgctcaaaac gcccgctctc cagctcaacg ccaattgcac gacgccccag cgccattgct
     1441 gctttgacgc tacggacata aaaaagccag ccactggggg aggctggcga aactcgtaga
     1501 gcaaaagtgt tgttacacta acttcgtcac agggttatcc tgcaatactc aaaatacaca
     1561 atatttacaa aactaatagt atacaaggcg atctttaaga ttttgttatt aattgtggtc
     1621 gcaccttcct ttctgtgtac tttccgtata gctcacagga ttctgggtac aaaaaaaccc
     1681 gcgcatcggc gggttcttaa atcttatcaa cggtagacat acaaagccca tcattgggaa
     1741 aatcttatcc atattttttg aaaaatgcaa gcat
//
LOCUS       DAZFJF010000336         1762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFJF010000336 DAZFJF010000000
VERSION     DAZFJF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.338"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            88..1464
                     /gene="ygeH"
                     /locus_tag="ACHKAU_005335"
     CDS             88..1464
                     /gene="ygeH"
                     /locus_tag="ACHKAU_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO5534185.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDILQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
     gene            1632..>1762
                     /gene="ygeI"
                     /locus_tag="ACHKAU_005336"
     CDS             1632..>1762
                     /gene="ygeI"
                     /locus_tag="ACHKAU_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO5534186.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQA"
BASE COUNT          644 a          298 c          298 g          522 t
ORIGIN      
        1 gcgttaatga cacataaata tgaatagcca taatttctat tgcttaagca ggatgcaaga
       61 aaccaatttt ttcatagagg ttaactaatg gacttagaaa ataaattctc atatcatttt
      121 cttgagggat taacgctcac ggaagatgga attcttactc aaggaaatga gcaagtttat
      181 attccacaga aagagttagg tgtattaata gtattacttg aatccgctgg tcatgttgta
      241 ctgaaagata tgatcatcga atcagtatgg aaaaatatta ttgttagtga cgagtccctg
      301 acaagatgta tctattcttt gcgctgcatt tttgaaaaaa ttggctatga tcgttgcata
      361 gaaacaatct accggaaagg ttatcgtttc agcgggcagg ttttcaaaac taaaattaat
      421 gaagataata cttcagacta ttccatagct atattccctt tcactacttc gttgaataca
      481 ctggatccat taatacttaa tcaggaatta gtgcaaatca tttcaaataa aaaaatcgat
      541 ggtctctata cctatccgat ggctgcgaca aatttttgta atgatcacat atctcaaaat
      601 tcattcttga gcagattcaa gccagattat ttcgttacag gaagaataaa ccagaataat
      661 gcagtgaaca ctttatacat tgagttgatc gacgctaaaa accttttcct catcgccagt
      721 aatcatctcc ctgttgatga actacataat acatcacaat ttattataga taatatcctt
      781 caaacggtac ataaaccaga acgatctgta agattagcta agcaggacca aggatataag
      841 aatcattatt tatcagatga aatgttagcc ggaaagaaag aactttacga cttcacccct
      901 gaaagcattt acagggccat gactatattt gatatattac aaaataaaag tgatatacag
      961 acgctaaaaa cagaatgtta ttgccttcta gcggaatgcc atatgtcttt ggcacttcat
     1021 ggaaaaagtg aacttgaact tgctgctcaa aaagcattag agcttttaga ttatgtatca
     1081 gacataacca ctgtcgatgg aaaaatttta gctattatgg gactgataac tggtctgtct
     1141 ggacaagcaa aagtatctca tatcttattt gaacaggcta agatacaccc aactgatata
     1201 gcctctctct actactatag ggcactagtc cactttcata atgaaaaaat tgaagaggca
     1261 aggatttgta tagacaaatc actacaactc gaacccagaa gacgaaaagc agttgtgata
     1321 aaagaatgtg tagatatgta tgtgcctaac ccgctcaaaa acaacattaa actctactat
     1381 aaagaaactg agagtgaaag ccatcgagtt ataattgaca acattttgaa attaaagcag
     1441 ctgacgagaa tttgtatgcg ataaattttt attgacgcaa ttttattatt tagatgtatg
     1501 caacttgtta tttaaacaac agagataaaa atatctaaga tattcacctt attgcaagat
     1561 atttaaaatg ctctagagtt aaaagtatga tgtaaagacc attggcctgg acaattaaaa
     1621 aagggagctt tatgactaat ccaatcggta tcaataattt atctcaaagt tcaaatatag
     1681 cgaatgcaac gggtgatgaa gtcgtatcat tagataaaca catcaacacc agtgctactg
     1741 atacagatca aatacaagca tt
//
LOCUS       DAZFJF010000337         1757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFJF010000337 DAZFJF010000000
VERSION     DAZFJF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.339"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(447..1424)
                     /locus_tag="ACHKAU_005337"
                     /pseudo
     CDS             complement(447..1424)
                     /locus_tag="ACHKAU_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          436 a          403 c          394 g          524 t
ORIGIN      
        1 tgctaatgtg ataaaaaaat atttatctcg atgctaccca acatacgact catttatttt
       61 tctcggtttc tccttgtata tatatgttgc tttataattt tactacgcac gtcaggcgta
      121 tctgtaacat agctccgcat tatgcaatgc gactttgcat acaaatagga ataaatctaa
      181 tagctgtaga tttgctcctg tatttcctga accaagcgag ccagtacatg atgcatatgt
      241 ctggaaaata attcagagta aggataagtc cagtccagct ttgttgaata aatcaggttt
      301 gggataagcc ccctcagttg gtagctactg caggtaatac gcacgtatta tgacattcgt
      361 gccctcgtca ttttgtttag tacacgcact gtagtggcac agaacttctg cgacctgacc
      421 atcctaaata gctgcgccga ataccgctac acttttaaca gcccatattt accaccgggg
      481 aatgggctca cggtttccat aaaataatga acctttttaa gcagttacca cattgaacgg
      541 cactgatgat tgcgcgttat cgtatcgtga agcgcctgct ataaccgctc aacatgattc
      601 acccacggcg agtaaactgg ctgataaatc actctgaact tcggattttc tttcagccag
      661 cgctgtgttt cccgactttt atggatgatg tagttgtcca ctatgagcgt gataggttta
      721 tcccgtcggt atgtcaattt cagatgtttc aacagactga taaataaggc tgagcttttg
      781 ctgttgccac ccacatagct gactttgccc gttccactat gtaaagcacc agccagatag
      841 tatttttcat tctgccccgg tgttaccaca tgtttttgct gtctgcgaag ttgccagtcc
      901 gcaccggttt tgggattaag atagatatac acttcatctt cataaaagac cggatgctcc
      961 gcactacatt cgtccagtgc tttgtgaatt gctgctttat gcgggttacg ggtacgcagg
     1021 gttggcgcag ccctgcgcca cacaagcccg actaatggca accagcggcg aacggttccg
     1081 gcatgtaact ggcactcggt tatctcgttg atttttattg ccagcagttc tgtactccag
     1141 cgtgaacgct gatagccaaa atatccggga gagtgtttta tcagttcacg aaacaatgtg
     1201 cagatatgct caaacggcca gcggcgggaa cgtcctgcgg gtaatgattt cagaccttca
     1261 acactcgaca gcgtgaacca gttaatccag cgcctgacgg aggaacgggc gcagcagagc
     1321 gttctggcaa cgtcactgac gcgagcgctc cggtgcagca tcagcatggc ggtcagcctg
     1381 cgggcataat ttttatcgtg agttttatag attgcttttt gcatcaggcg tcattcgtcg
     1441 cggggaattg gtgctatgat cggcatcgct cagtccggtt agggggggat ttttggcgat
     1501 tgatcagatc gcacaatccg ggctgagttc cctttcattg atctactatt cggcgcagct
     1561 atttagttgg tgattttgaa cttctgcttt gctactaagc agcctgcgtt ttcaggaaaa
     1621 tacgtgatgt gatcctgcaa ctcagcaaca gttcggttga ttcaacaaag cgttttttaa
     1681 caataattct tcattgtaaa aagcatcttc agttgtgatc aaatattatt attcttattg
     1741 gtgttctcat ataatga
//
LOCUS       DAZFJF010000338         1754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFJF010000338 DAZFJF010000000
VERSION     DAZFJF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.340"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..102
                     /locus_tag="ACHKAU_005338"
     CDS             <1..102
                     /locus_tag="ACHKAU_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO5534187.1"
                     /translation="IGVLGSLLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            106..354
                     /locus_tag="ACHKAU_005339"
                     /pseudo
     CDS             106..354
                     /locus_tag="ACHKAU_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            1409..>1754
                     /locus_tag="ACHKAU_005340"
     CDS             1409..>1754
                     /locus_tag="ACHKAU_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO5534188.1"
                     /translation="MNAKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLTKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGP"
BASE COUNT          489 a          365 c          462 g          438 t
ORIGIN      
        1 ataggtgtgc tggggagtct gctgtttggc ctgctgacgt atcttactaa cctgtatttc
       61 aaaatcagag aggaccgtcg taaggcggcg cggggagagt agacgatgaa taaacaatac
      121 gaactggttg ttaaagggat aaatatttac ccggataaga ttactgttac tgtggcgctg
      181 gaaactggtg ggtatacgtc actgttgttg ccaaatgtgg tgattgatct tgatcgtgtt
      241 gaaggtgccc cgctggagtt ttacgaagct gaggcgaaaa agaaggcgaa gcagtttttc
      301 atggatattg ctgccgggtt atgtgaaggg aacgaaccgt tgccggaaaa gcgcctctgt
      361 tcagaagagc ggtataccat acaaattaac aatgcatata acacgataca gtcagaaaaa
      421 gatgatatcg aatcacgcat tgaaaaactg gagaacagcg tcgttgaact gaataaaaaa
      481 ctgtcggtgc agatcccttc cgaaggtgaa aaaaaacgcc gtgatgagca gttcgcggcg
      541 ttttacgatt attgccggaa agtgatgagc agaaatctcg cagagtgttt ccgtattcaa
      601 aaaaacagag agcgctctgc aaatagtgag attctggata attttttaaa ggggacgcat
      661 ctcgccagtg attgtttcat tgctcattct tttggtggct ttcatgaggc catcaaacat
      721 gatgttgaga gaagtccagg tatcctctgt tccattcccg taagcatcga aatagacaca
      781 aatacgataa ccggtgaact cgttaccgcc ggagtcggta tattccgtca tgaggggaac
      841 gatgccaaag gttcctttac aaaaatcggg ccgttctttc tgaaggtaga gcgatagttt
      901 gttaagtacc catccgacag ctcgctcatt ttggtttatt ttcgtatcgc gaaacaaaaa
      961 ttcgaagcgg ccagatgacg catgggtgca accaaatacc ggggcgtatt catcaacctt
     1021 tggtgttcct gaaaacatgc agtccgtgct ctcgaatgca gatgaattgc tgttgatcca
     1081 cttaacaaaa gaaaaaaagg tctgaatcgc ttgtctgtct gaatattcaa caaaatcctc
     1141 aaggtgtgtc tcgatcagtt ccgggtgttt acgaaagtca taatattgac cgggacgata
     1201 tttatgagtg gttgttttat cccatggttg tgctctgcgt ccctgtaaac ggaggtctgc
     1261 cgctggatag ataagcataa gtaatctcct ggtggaatgt aatccacgat catccgggga
     1321 aattaagaac ccgccagtgc ccaccactgg tggggtgaag gcttaacata tccagggatt
     1381 cggaaccgat aaatcctgat aaatatccat gaacgcaaaa atcaaatacg gcctgtcggc
     1441 tgccgttctg gcgctgattg gtgcaggggc gtctgcgcct gaaatcctcg accagtttct
     1501 ggatgaaaag gaaggtaacc acaccacggc ataccgtgat ggtgcgggta tctggaccat
     1561 ctgccgtgga gccacccggg tggatggtaa gcctgttatt cctggcatga aactgacgaa
     1621 ggaaaaatgc gaccaggtta acgctattga acgtgataag gcgctggcat gggtggagaa
     1681 aaacatccgg gtgccgctga ccgaacccca gaaagcgggg atcgcgtcat tctgtccgta
     1741 caacattggc cccg
//
LOCUS       DAZFJF010000339         1721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFJF010000339 DAZFJF010000000
VERSION     DAZFJF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.342"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..266
                     /locus_tag="ACHKAU_005341"
     CDS             <1..266
                     /locus_tag="ACHKAU_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5534189.1"
                     /translation="DKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAERQLTKWAK
                     HVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            300..1025
                     /locus_tag="ACHKAU_005342"
     CDS             300..1025
                     /locus_tag="ACHKAU_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5534190.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            1041..1433
                     /locus_tag="ACHKAU_005343"
     CDS             1041..1433
                     /locus_tag="ACHKAU_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5534191.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            1430..>1721
                     /locus_tag="ACHKAU_005344"
     CDS             1430..>1721
                     /locus_tag="ACHKAU_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO5534192.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVA"
BASE COUNT          447 a          379 c          542 g          353 t
ORIGIN      
        1 gtgataaata cccgtggcca cagcctgtgc tgtaccacat ctgcctcgaa atgcgtacca
       61 gagggattga gcgccagatg acgcagggtg agttaaaact acttgcagaa cggcaactga
      121 cgaaatgggc aaagcatgtt ggtaacggga tgagtgttcc gccagtgcga cgacaactgg
      181 aaggggcgaa acacccgcaa gggccaacgc caattgagcg gctgaaacag gaatacgaac
      241 gccggaaggc agctggtttt atttgaatct gagaaacgat tttgtcggag gaaattttaa
      301 tggaaaccgt atttgacgca ctgaaagcac tgaaaagagc ctcttcacag gtagtggcgg
      361 cccgccttgg aatcagccgt gaagatgcgg tcaacgaact gtggaaactg aagcgccgcg
      421 gtgaagcgga taacaagggg tcgatgtggt ggctgattca ggctggtgaa agtgaaccgg
      481 tgtcaccggt accgaaagtg acagcgcaaa tgctgactga ggcgattgaa caacatggcc
      541 cacaaacggc ggatgagctg gcactgatgt tcgggattac ctcccgccgg gcgaattcat
      601 cgctggccat ggtaatcagc aaagggcgtc tgattcgcgt gaatcagggc ggtaaatttc
      661 gttactgcat accgggcgct gatttaccgg cagagccgga agctgcatcc gtagcggaaa
      721 ccgatggtaa agcctttcct cagccagcag gtgttgcgtt accagttcgg gaagcagaaa
      781 cacaggaaga aataaaaacg gaaagtgtgg cggtcacagt gcagtcacag ccgtcgttca
      841 ccagaaaaca tccggatggt ctgattttac catcgctgca tgtggctaac cgcgagctgc
      901 gccgggcaaa aggtcaggtt cagaagtggg agcgagtctg cgccgcgctg cgggagctga
      961 acaagcaccg ggatattgtt cgacagatta ctgattcttc ccgccgtgtt gtatcggaaa
     1021 agtgattgcc ggaggcgctt atggcaaaag aatttacacc agaagagcgg gaagaagtga
     1081 aggcgcgcat tgtggaattc gtgcgcctga gcggacgaga aacttttcga caactggcag
     1141 ataaaacggg tgtcagtaag accgctattc gtcgtttatc tggtgcgctt gcggccagtg
     1201 gtgatgtctg gctctctggt tgcggggtat ttccatcaga gcaggcgtat cgcgtatggc
     1261 gtaagacacc ggagaaggct gctgacccga cactgattcg aaagttacct gacggagaaa
     1321 tacgtcgtta caacagacgg cagaacataa tttgtcgtga gtgccgccag agcgaagtta
     1381 tgcagcgtgt gctggcgttc tatcggggaa actttcagga ggtgatggag tgagggtgag
     1441 agtttatatt gccggtccaa tgacgggata tgaaaatttc aaccgtgagg cgtttcacaa
     1501 ggcggaagag gaactgaaac gggaagggca taccgtctta aacccggcag tacttccgga
     1561 cgggctgaca cagccgcact acatggatat ttgcatggca atgattcgtt gtgtggatgc
     1621 gatttacatg ctgaatggct ggcagcggtc agcgggcgct aaggcagagc tggcactggc
     1681 ggagaaactg gggcatgcgg tgatttatca ggaggtggct c
//
LOCUS       DAZFJF010000340         1720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFJF010000340 DAZFJF010000000
VERSION     DAZFJF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.343"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..84)
                     /locus_tag="ACHKAU_005345"
     CDS             complement(<1..84)
                     /locus_tag="ACHKAU_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5534193.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQ"
     gene            complement(152..>1720)
                     /locus_tag="ACHKAU_005346"
     CDS             complement(152..>1720)
                     /locus_tag="ACHKAU_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5534194.1"
                     /translation="QAKWDTPKVVKGVSFMLRLTVVADDGSERLVSTARTTETTYRFT
                     QLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITAVPRLAVYD
                     PTVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGK
                     SAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVS
                     NEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIE
                     NTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGN
                     PPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTV
                     GKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRL
                     IVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLL
                     VVVMKKSTAGISIS"
BASE COUNT          382 a          504 c          402 g          432 t
ORIGIN      
        1 ctgctgctca gacgcccatg caggcgcacc ggatacggcc agacagatgg ctgcggacaa
       61 aatggctgca caaactttac gcataattac ctctcgcttt tctgcaataa aaaaggcgcc
      121 atttctggcg cccgtatatg ggttataaaa ttcagctgat actgatgcct gctgtggatt
      181 tcttcatcac cacaaccagc aaatcgctga tacttgctgt gggataccag ttattcacca
      241 gccatgctga taccgaaaac tccagcgtca tgtgaccgtg accggcaggc atatcaataa
      301 cgccactgta aatcagcgta ttatccagcg cggtacggtt ataaatttca gcaccgtttt
      361 tccgcactat cagacggcat gaggagtaaa tatcagtatg ctctctctca tgcttagcgc
      421 cactgaatgc caccgccgga ataacaatct gccggtcaaa cggctgatcg tcataaaccc
      481 tgacggtaat ggtccctgat ggccaccgct ccggtgcccg ggagtcccgc ggaaaagcct
      541 tacccactgt tttgactata tcgccttcaa tctggttggc tgacagtttc cccttaatct
      601 gacagttctc attaattgtg acattgttga gcgtcccggc gttcgcattc acactgccac
      661 tgatatctgc atttttagcg gtcagctttc cgtccggtgt cagggaaaat gccggaggat
      721 taccgccgct ggtaatggtg ggagccgtca ggcgtttcag gaacacgtcg ttcatgaata
      781 tctgatcgcc ctgaccaaca aacatcggct ttgtgttgcc attcgcagga ttaatcatcg
      841 caatcctgtc tgccgccagc agcacctgac tctgcattcc tgctggcgta ttctcaatac
      901 cggcaccgat acccgcaata taaaggcgtc cgtccttcat ctgttgcagc ttcacagccc
      961 acatgctgtt cagattatta tttgtatcaa cctgaacctt ctgtatctgc tggatcgctg
     1021 cactctggtc ttccagtttc ttattgacgg tctgcgtgat ttcattactg acatccgtga
     1081 tggacgttct gatttccgcc aggtcaggcg caagctgacc gttatcaatc tgagtccaca
     1141 actcctgagc cagatgggtt ttccctatct ctcctttgaa aaaatccaga tagccggatg
     1201 catcatcact cggctggcca acagcctcca caaatgccga tttgccaacg gtgttcacac
     1261 tgcggatata aaagtaataa tcatggcccg gtttgatatt gatactggcg gctatccagt
     1321 acagccccgt gccaaggtag cgggctgtgg tttcaacctg cctgatatcg gtaatccgcg
     1381 tttccgaaaa ccagaactca aactgtaccg tcgggtcata caccgcaaga cgcgggaccg
     1441 ctgttatctg aaaatagccc ggtgtcagct caatctgtga cggcgctgcc ggtgcggcaa
     1501 tccggaacga taccgacgcc ggatcgccct gctgccccca cgcatttacc gcccggactg
     1561 tcagcctgta gttccccggc gccagttgcg tgaagcggta tgtggtttcc gtcgtccggg
     1621 ccgtgctgac cagccgctca ctgccgtcat ccgcgaccac ggtcaggcga agcataaagc
     1681 tcacgccctt caccaccttc ggcgtgtccc atttcgcctg
//
LOCUS       DAZFJF010000341         1712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFJF010000341 DAZFJF010000000
VERSION     DAZFJF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.344"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(10..591)
                     /locus_tag="ACHKAU_005347"
     CDS             complement(10..591)
                     /locus_tag="ACHKAU_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5534195.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEVLQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLTGEGLP
                     HPLTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            complement(882..1457)
                     /locus_tag="ACHKAU_005348"
     CDS             complement(882..1457)
                     /locus_tag="ACHKAU_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5534196.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            complement(1571..>1712)
                     /locus_tag="ACHKAU_005349"
     CDS             complement(1571..>1712)
                     /locus_tag="ACHKAU_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5534197.1"
                     /translation="GIIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGG
                     MK"
BASE COUNT          483 a          456 c          288 g          485 t
ORIGIN      
        1 atgttcattt caattaccct ttataacgaa gtttcctctg gtatcgtcat aaatgcattc
       61 ttcatgtttt acaattattg atgccgttat tggttcccgg gtcagtgggt ggggtaagcc
      121 ttcaccagtc aaacgagaaa atgcagcggc atcaaataaa gtacatacat ctgaaccatc
      181 tgaattcttc acaaaaatac ctttttcagg ctgctccagt gtaattggac attgaagcac
      241 ctcaccagga cagtgaagtt tttcaatatc aacagtaaag gtacattccg atattttggc
      301 ctgcaaagag ttctcttgtc gctgcgccac cattccagga agcacgctta acaccctgtt
      361 tctgagcgca tcaactccat cattaaaacc aatattcaat aattctgcat aaaccagtcc
      421 cagcccttca ctttctgcta aaaaacggcc tgtatctggc atataagaaa ctctaactct
      481 ctcccctccc aggagaaatc ctccaatctg cccttccatt acctgctgac gcatttccgc
      541 catctcattc gcgaatgaag tcgatgatat atatgatact aatccaggca ttaccatatt
      601 ccttcataca gataaatatt tcctctgggc ttcataacaa acatctccct gacatgacaa
      661 caaaaaccgg agccggactc cggtttttgt gaatccgtcg gctatttcat cccgccaata
      721 tttccccgtc agcacgccag atttgcagcg gcctcaccac tcactgtata tgctttttag
      781 ctgcatccag tacaccaatt accacatcct tataccctga acatctgtag catttcatat
      841 aagcaactgg cagttaacat atagcttggg taatatttat ttcacttatc ctttatgaca
      901 aagtttcctt tggtttggtc ataaatacat tgttcttgac ttacaatcat tgatgacgtt
      961 attggttctc gtgttagcgg gtgcttccca ccgtcgcgaa ccaaatgaga aaaagaaacc
     1021 gaatcaaata aggtacaaac caaagaacct tctgaatttt taacaaacac accttcttct
     1081 ggtttatcca gtataattgg gcatcgaacc gcctcggatg gacactgaag gctctccgta
     1141 ttaacagaaa acttacattg tgatattttc ccctcaatat caccatgtga ttgtgtgtta
     1201 cctgaacctg aaaccattct taacattata tccctgagcg cttcaggacc accattaagc
     1261 cctgtaagta ataatccaga aagcaaactg ttattgcccc cactcgccag aaagcgacca
     1321 tccgtttcac tatatgtgat acggaccgtc tgcccgccta ctgtaatttc tccaccgcca
     1381 ctggcggcca tttgagcacg aagaacctgc attccggtgg aaaatgaatt ggatgcaata
     1441 gctgatgtta acggcatgac aaaactccct atttaaatat tcattccaaa cacagggagt
     1501 cataacaaac agcccccata catgacaaca aaaaccggag ccggactccg gtttttgtga
     1561 atccgtcggg ttacttcatc ccgccaatat tttcccacgt cccgtcagca cgcagaattt
     1621 gcagcggtct taccacacac tgtatctgct ttttatccgc atccagtatc accacctgcg
     1681 tgattaccct gtcctgctcc gggataatgc ca
//
LOCUS       DAZFJF010000342         1703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFJF010000342 DAZFJF010000000
VERSION     DAZFJF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.345"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(372..448)
                     /locus_tag="ACHKAU_005350"
     tRNA            complement(372..448)
                     /locus_tag="ACHKAU_005350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Arg,seq:tct)
     gene            complement(462..538)
                     /locus_tag="ACHKAU_005351"
     tRNA            complement(462..538)
                     /locus_tag="ACHKAU_005351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(502..504),aa:Arg,seq:tcg)
     gene            complement(548..623)
                     /locus_tag="ACHKAU_005352"
     tRNA            complement(548..623)
                     /locus_tag="ACHKAU_005352"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(588..590),aa:Ile,seq:cat)
     gene            complement(664..777)
                     /locus_tag="ACHKAU_005353"
                     /pseudo
     CDS             complement(664..777)
                     /locus_tag="ACHKAU_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(1126..>1703)
                     /locus_tag="ACHKAU_005354"
     CDS             complement(1126..>1703)
                     /locus_tag="ACHKAU_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5534198.1"
                     /translation="DPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFADY
                     SRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTAC
                     RGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQW
                     YKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
BASE COUNT          450 a          442 c          358 g          453 t
ORIGIN      
        1 aatgacaatg caagtatctc atgctgttgc ccgaacctac tcgggctttt tttgcatgta
       61 aaaaggctcc tgcgatgagg ggcctggata tatgcctaat ctctgtatac agcatgatgc
      121 cgggtgcctc ccggtgaatt ctgcaatgac cagacagaat ccgcaacttg cctatacaat
      181 acgcaaccaa acatctgtca ttatgccccg ccgcccaggg ggattcatca tgcaggattt
      241 ttttaacaaa cgctcagcat gtcaggcaac agtcgactac ctgaattgtg aggcatttaa
      301 catttcactg tccggtgtct ttcctgtaat aaaaagcccg caaaagagag tcagggcaga
      361 taagtgtggt gtggcgcgtt gtactggatt cgaaccagtg accgattgct tagaaggcaa
      421 ttgctctgtc cggctgagct aacaacgcat gatgctgata atggaccgcc atcggggact
      481 tgaaccccgc acagccagct tcgaaggctg acgctctatc ccgatgagct aatggcggta
      541 tgtgatatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
      601 tctcaccact gagctaaagg gccgggcgca ggataataac ggtacgtaac taatcctgca
      661 atatcatccg ttctgactga ctaaatcctg aacttccctg accgtctgct caaaacgttc
      721 agtctccagc tcaacgccaa ttgcacgacg ccccagcgac attgctgctt tgacgctaca
      781 gacataaaaa agccagccac tgggggaggc tggcaaactc gtagagcaaa atgctgttac
      841 gcaaacttcg ttacagggtc atcctgcaat acaaaaaata cacaatattt agaaaactaa
      901 tagtgccatg tgcaattttt aagattttgt tattaattgt ggtcgcacct tcctttctgt
      961 gtactttccg tatagctcac aggattctgg gtacaaaaaa acccgcgcat cggcgggttc
     1021 ttaaatctta tcaacggtag acatacaaag cccatcgttg ggaaaatctt atccatattt
     1081 tttgaaaaat gcaagcatta tgtcgtcatc ttcggcgaaa accatttatc ttgtcacctt
     1141 tctcaattgt atctctgcat atgcttcttc ctgccagcac tttgtaacca gtttatcaat
     1201 gacatctgca tatcctttgt accactgata atccgtcagg tctggtacca gcttctggac
     1261 atgaagccgc gccagtgtgg ttggtaaacg gctaaaccgg tttccattgc aacgcccaca
     1321 aaccttataa acaggcgtgc catgaagccg ggttcttttt tcatccagga caataccttt
     1381 acccttgcac cctctgcatg ctgtgctgac ttctccctta ccatgacaat gctgacatag
     1441 ttccttcacc cactcttcct taataacaga ttccccgctt ctggagtgtt tcaccacctc
     1501 gcgcaataca ttatgaaatc cagtaccagc acaatgctca cagcgagcct tacttgccgc
     1561 agacctggaa taatcagcaa aggcaaaatt cacaaggtaa ggaatgatct gtagccgggt
     1621 ttcttcactc aatttattca atgtcgggtt atccagtgcc atcgcgtaat tgagcagacc
     1681 ttcaatcgca aactgaggat cct
//
LOCUS       DAZFJF010000343         1678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFJF010000343 DAZFJF010000000
VERSION     DAZFJF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.346"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..411
                     /locus_tag="ACHKAU_005355"
     CDS             37..411
                     /locus_tag="ACHKAU_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5534199.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            408..1229
                     /locus_tag="ACHKAU_005356"
     CDS             408..1229
                     /locus_tag="ACHKAU_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5534200.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1467..1678
                     /locus_tag="ACHKAU_005357"
                     /pseudo
     CDS             1467..1678
                     /locus_tag="ACHKAU_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
BASE COUNT          437 a          336 c          483 g          422 t
ORIGIN      
        1 tttgcaaccg gcgtgctcgg gtaaaagagg ttactgatgc gtatagagtt tgttttgctt
       61 tacccgccga cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      121 gccggtgagc gttatcgccg ggctgtggcg cttattgttc gccagcagcg gctgaaatta
      181 agcctgtccg gaaggctggc gataaagatt attgccgagc caccggataa gcgccgccgt
      241 gacctggaca atattctgaa agcgccgctg gatgcgctga cgcatgcggg agtgttaatg
      301 gacgatgagc agtttgatga aatcaatatc gttcgtggtc agccagtatc tggtggacgt
      361 ctgggggtga agatttaccc cataatgctt gaagggctgg tcaaaaaatg aaactggaag
      421 atttaccgaa atactactcc ccaaaatccc ccggcctgac tgatgcatcg gcctcaacgt
      481 cgaaagatac gctgagtatc actgatgtga tggccgcgca gggcatgaca cagaattggg
      541 ctgagatggg gttttctgcg ttccttggga aaatgggcat tagtatgaat gacagagagc
      601 gggcaacaga attgctgaca gaatatgcac tcagtcggtg tgatcgcgtg gcggcgttaa
      661 gaaaactccc ggcagaaata aaaccggcag tgatgcgtat tatggcttcg tatgcgtttg
      721 aagattatgc ccgtagcgcg gcgagcaaaa aacagtgccc ctgttgtcac ggaaaaaaat
      781 ttattgaaag cgaggttttt acaaacaaga tccagtatcc ggatggtaag ccgccagtgt
      841 gggcaaagtg cacaaaaggc gtgtatccgt cttactggga ggaatggaaa aaagtcaggg
      901 aggtggtaaa agttgcctgt ccggagtgtg gagggaaggg ggaggtttcc accgcctgta
      961 aagattgtcg tgggcgcggt gttgccattc atcgtgaaga gtcggtaaaa cgtggtatgc
     1021 ctgttatcag agactgccag cgttgtggtg gtcgtggcta tgaaagatta ccttcaacgg
     1081 aggcatttaa tgccatatgt aatgtaaccg atgccatatc tcttgataca tggaaaaaaa
     1141 cagttaaacg tttttacgat acgctggtgg tgcagtttga tattgaagaa gcatgggcag
     1201 aacaacaact gaaaaaggtg accagatagc tttgttgatt tttcccgaat ctgtggtaaa
     1261 tttgccctaa cgatgggcgt tttatgcctg acgttagaag attttttaca cccgtcgcca
     1321 ggcgggtttt tttatgactg aaatcacgcc agtacagtaa acgcgctggt ggttgtgaat
     1381 accggtcttt cagcttgctg gctttttcga caagagttat tggtgtgtca cgttaaccgg
     1441 aaaaaggaaa gtttgagaaa cgcgatctgg cacaggcggt tattaatgct gcctacctgg
     1501 tggcctgtgc agatggtgaa tgtgaggctt cctagaaagc gaagatcgaa caggtactgc
     1561 gtaatcagcc tgcgctgtcc gcgtttacgt cagaaattaa tgcgattagc gcaaccatta
     1621 tcggtcagct ggatacgaac tttaaaattg gtcgtcgtgc ggcgttacgt gagatcga
//
LOCUS       DAZFJF010000344         1643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFJF010000344 DAZFJF010000000
VERSION     DAZFJF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.347"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(12..1564)
                     /locus_tag="ACHKAU_005358"
                     /pseudo
     CDS             complement(12..1564)
                     /locus_tag="ACHKAU_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1561..>1643)
                     /locus_tag="ACHKAU_005359"
     CDS             complement(1561..>1643)
                     /locus_tag="ACHKAU_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5534201.1"
                     /translation="GGDRVATSQTDRTAWHPDITRDKTGE"
BASE COUNT          301 a          472 c          455 g          415 t
ORIGIN      
        1 tggggtaaaa attagtttac agattgagtg acattccagg gcaacaactc tttcacgcgg
       61 ttggcaggcc aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt
      121 ccgttaagtt tgcagctacc gatcaggctg tacatcactg ccgcactctc gcctccacca
      181 tcagagccga agaacatgta gttacgccgc cccagtgcaa ctacccggag ggcgttttca
      241 cacaggttat tgtcgatctc cacccagcca ttgcggcagt attcgttcag agcgtcccat
      301 tgcttcagca gataggtgaa cgctttcgct gtatccgagt ggcgcgacag cgtgctcatc
      361 tgcccctgga gccactcata caacgactgc attagcggta ccgttctggc ttttctgacc
      421 gccagtcgct cttctgccgg actgccgcgg atctcagcct cgatagcgta cagttccccg
      481 atacgacgga gcgcttctcc cgttactgtc gttggatggc ggacatgtac atcgtggatc
      541 ttgcgtcggg catgtgccat acaagccgct tcggttacct gaccgctttc gtaaagagca
      601 ttgtaacccg catatgcatc ggcctgcagg atacctctgt agtccgccag atgttgctgt
      661 gggtggatgc ctttgcggtc gggagagtat gagaaccaca ccgctgccgg catggtcgaa
      721 ccggcgttgc gatcgtcacg aacatagacc cacagccgtc cggtacgggt tttaccctga
      781 cccggctcca gtacatttac cggcgtgtca tcggcatgca ctttacccgc catcagcaca
      841 tagtgcttca gttcatcata cagcgggcga agctgctctc ccatgatgtc aacccagcgc
      901 cccatcgtat tgcggtgcag ctccacgccc tggcgggcat agatttccga ctgacggtac
      961 agcggcagat gctcggcgaa cttagccatg attatgcggg ccagcagagc cggactggcg
     1021 taactgcgct cgatgggttt tggtggctgc ggagcctgaa ctatacagtc gcaccggctg
     1081 caggccagtt ttgggcgaac cgtttcgatt accctgaacg cggtgttgat gatatccagt
     1141 tgttcagaga tgctttctcc cagcggtttc agtttgccgc tgcagacggg gcattcggtt
     1201 tctgccgggg agataacctg cctgtcacgg ggaagtgttg ccggaagtgc tttgcggacg
     1261 ggagagtctg atgttttcgg cgctgtctct ccggccattg aggtgagttg caactgcgcc
     1321 tcaccaagcc tgttctggag ctcggttata cgcgtttctg cccgtgcgat cttcttttct
     1381 atcttctcgc ggcttttctc gctgctgcga ccgaacaaca ttctctgcag tttagcgacc
     1441 agcgctctga gtgagctgat ctcgcgggca tagccggtta tttcaccaga cagacgataa
     1501 cagcctgctg tgcgatcagc agggccttca gttgctcgat gtcgtcgggg agtgtgttgt
     1561 tcattcccct gttttatcac gggttatatc cggatgccag gccgttctgt ccgtttggga
     1621 tgttgccacg cgatcccctc cag
//
LOCUS       DAZFJF010000345         1601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFJF010000345 DAZFJF010000000
VERSION     DAZFJF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.348"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            31..342
                     /locus_tag="ACHKAU_005360"
     CDS             31..342
                     /locus_tag="ACHKAU_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFO5534202.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
     gene            418..552
                     /locus_tag="ACHKAU_005361"
     CDS             418..552
                     /locus_tag="ACHKAU_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534203.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            537..689
                     /gene="kil"
                     /locus_tag="ACHKAU_005362"
     CDS             537..689
                     /gene="kil"
                     /locus_tag="ACHKAU_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5534204.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            765..935
                     /locus_tag="ACHKAU_005363"
     CDS             765..935
                     /locus_tag="ACHKAU_005363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534205.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            946..1551
                     /locus_tag="ACHKAU_005364"
     CDS             946..1551
                     /locus_tag="ACHKAU_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO5534206.1"
                     /translation="MSKDFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
BASE COUNT          512 a          334 c          393 g          362 t
ORIGIN      
        1 tgtgagggcg atttttttga cggaggaaat atgaaattac gtgtctggca tatcccgcaa
       61 gtacctatga aaccgttcat tgcagaagtg gcaagtgttg aagagggtgt tcgcctgatg
      121 ggcgcactgg ctgattatga cgcctttcag tatgacaaca acatcaagcc tgattactgc
      181 aatgctaacg gccttgagat gtgggatgag agccttaccg atgaagattt atcagaggtg
      241 gggcttactg atcgctgggt ggattggtac agcgaatgcc aatgttacga cgacccacgt
      301 aaatatctcg aaagcctgaa agaagaaaca tcagccgcct aagcgcggct ttaccgcata
      361 ccaataacgc ttcactcgag gcgttttcgt tatgcaatca aatataagga gttacccatg
      421 atgcactttc agctcgcggg tagcggcgtc atgtccgctt tctacccgca cgaatctgaa
      481 ttatcacgcc gagttaaaca attaattaga gcagcaaaga aacaactgga ggcgttatgc
      541 gcaatgaaat agccattaat caccagatgc ttcgtgcggc acaaaacaaa gcagtaatag
      601 ccagatttat tggtgattca aaaatgtggc ttgaagcaaa taaagcgatg aaatcagcta
      661 tcaaccttcc gtggtatcgc aggaaatgag tttcacagat aactggtcag acgaagaatt
      721 cattcgtcag atgaaagaat taatcggtaa cgaaggagat attcatgtca cttgcaacca
      781 cagtgaagga gagcaagtta cagagacgca tgtacacgca gcaggcgtta atgtatcgcc
      841 agaagggaga tcgtgaaggt gttcgcgtat ttttaaatgc ggcaaagact gaagtattaa
      901 atcagcgtta tttccttggg ccatgcccat tctgagaaca aacatatgag caaagacttt
      961 tacgcaagac tggcagctat tcaggagaac ttgaacgcgc caaagaatca gtacaactca
     1021 ttcggtaaat ataaatacag aagctgcgaa gacattcttg aaggcgttaa gccgttactg
     1081 aatggcctgt ttttatcaat cagcgatgaa gttgtgttga ttggtgatcg gtattatgtg
     1141 aaagccacgg caactattac cgatggcgaa aacagtcata cggcaaccgc tcttgcacga
     1201 gaggaagaaa gcaagaaagg aatggattct gcacaagtta cgggagctac aagctcttat
     1261 gcacgcaagt attgcctcaa tggtttgttc ggcattgatg atgcgaaaga tgcagatacc
     1321 gacgagcata aacatcagca gaacgcagca gcaaagcagt caaaaccatc acctacacct
     1381 gaacaggttc taaaagcatt cactgacgca gcattgcaga aaaacaccgt ggaagagctt
     1441 aaacaggcgt tcgccaaagc gtggaagatg ctcgaaggca caccggagca gcacaaagcg
     1501 caggacgttt acaacatcag acgagacgaa ttagaaggag ctgctgctta atggcacatt
     1561 cgattactgt aagactaaac aagcccgcaa gagagtttca g
//
LOCUS       DAZFJF010000346         1595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFJF010000346 DAZFJF010000000
VERSION     DAZFJF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.349"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..458)
                     /locus_tag="ACHKAU_005365"
     CDS             complement(<1..458)
                     /locus_tag="ACHKAU_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5534207.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KREPVTWQGRQYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLA"
     gene            complement(458..799)
                     /locus_tag="ACHKAU_005366"
     CDS             complement(458..799)
                     /locus_tag="ACHKAU_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534208.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(792..>1595)
                     /locus_tag="ACHKAU_005367"
     CDS             complement(792..>1595)
                     /locus_tag="ACHKAU_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5534209.1"
                     /translation="DADLQSQYQTGDIGEDFYRQALAQNAQYLSERLKEQEAFYAESD
                     VQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWA
                     SSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYTSANL
                     SAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVY
                     SPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          331 a          576 c          347 g          341 t
ORIGIN      
        1 gccagcacaa acgaggctgt catggccgtc agctctgaca tctgctccac cacccagcgg
       61 tcgctcagct cctgctccgg gtccgcctcc ggattgcccg caacgaaatt caccgcatcc
      121 agaaaacggg catacacccg gcggcggacc accgtggccc ccaccaggct ctgcaaatcc
      181 tccgccatcc cggtgacaag accgaacaga ttggacaccg tcagcgacgg gcgggcactg
      241 ctgccctttc cgttcatctc aaagccactg ccctcaatcg ggtacgcctg atattgccgc
      301 ccctgccagg tcaccggctc ccttttttca ttcagctcat tgcagaaaaa ataccgctca
      361 ccgccctgca ccgtcaggtc gatttcccag agcaccaccc gcggtgactg ctctgactta
      421 accgactcgt tcaggctttc ttcgtgaata tcctgcatca gttcaccacc tgctctatcg
      481 tgcaactgaa atcactgtac cgggcattat ccgtgacact ccactcacgg cacacaaccc
      541 tcaccgtccg gttatgtttc ggcggtcgcc acaaaaaggc acggtaacca ccatgccacg
      601 ataaaaactc ttccagccag cgcctggttg actcatccgt cacccggaac accgcctgaa
      661 acgtcttcag ttgaggattc agccctgtgg ggcggcgctg ttcataaccg tcaccaaacc
      721 gcaccctcac caccgacggc ttctcactca cctgcatccc ttcacgcggg accagatgca
      781 gcgtttttat ctcagccact cagcattcct ccgtcacgtc gcatggacag catcaccgcc
      841 tgcacccgct ggtcaatcag ctgcacaaga ctgcctgccg cctccggccc tatctggcca
      901 ttagtcccgt cattctgaat ggcgatatgg tagaccgggg aatacaccag acccgcactg
      961 ccgttcatac tgcccaccgc gcgcacaccc agcgagccat ccgccgcccg cgtcagaggc
     1021 ataatggctt caggtccggc ctcccccatc agcccggccc cttttgcaaa ggcaaagtac
     1081 gtgggcgtat ccacaatact gttgctgtac gcactcaggt ttgccgaggt atacacgccg
     1141 ccttttgcat tggccaccgc cccgcccagc cagtcaccaa tgctgccgag aaatcctccc
     1201 gcaccggaca taccgtttgc cgccgtctta attccgttga caatcgcggc attcataaga
     1261 acttttgata tttcctgcag cactgatgag gcccagctgc gccattccac tttattcccg
     1321 ttcagcatct ccgtgatgtt attcaccatc cctgagatac cctccgtcgc aagctgtgct
     1381 gcctgtgagg cgtaatcgga cgcattatcc acccagttac tgaacccctc ctgcagccct
     1441 ttctgccagt ccgcacgctg cacatccgat tcggcataaa aggcttcctg ctctttcaga
     1501 cgttcactca gatactgtgc attctgcgcc agagcctgtc tgtaaaaatc ctcaccgata
     1561 tccccggtct gatactgaga ctgaaggtcc gcatc
//
LOCUS       DAZFJF010000347         1594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFJF010000347 DAZFJF010000000
VERSION     DAZFJF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.350"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            192..692
                     /locus_tag="ACHKAU_005368"
                     /pseudo
     CDS             192..692
                     /locus_tag="ACHKAU_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            694..987
                     /locus_tag="ACHKAU_005369"
     CDS             694..987
                     /locus_tag="ACHKAU_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO5534210.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(1048..1594)
                     /locus_tag="ACHKAU_005370"
                     /pseudo
     CDS             complement(1048..1594)
                     /locus_tag="ACHKAU_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          529 a          289 c          243 g          533 t
ORIGIN      
        1 caaattatga tgacacagaa taaaaaaatt ataattccga gaactatttt aaatgcctct
       61 atgcataact ttatttacaa tttaaggtat aaaccctatt ccctcaatgt taagcaaata
      121 taacaattac tcttatttta aattttacac gtaatattta ccctaaatga ttttagagta
      181 caggtgggca tatgcaagtc ttttccagtg atgtctactt cactgtaggc actaatgcat
      241 tattagcatc ccaaaaagaa tattacagcg atttagttgc gttggttgat cttggacatt
      301 cgttcgttgt tattgatgag catcaacatc gaaatctcaa accaaataca gaacctgtaa
      361 acatcctttt gagcaacaat ttcatcagga tcaataagaa tattacatta tctgatttaa
      421 cgcatttcct ggtttcaaat cttcatacac aaaatgtgta ttccacacaa gaaccactta
      481 cccatgatga gatagatatc ctgcggttat gtgtttctta ctcgttaaaa caaatagcca
      541 ttattaaagg aattgattat aaaacaatat catatcacaa aataagagcc ctaaataaac
      601 ttaacataaa aggtactgtt tagttgttta tagcactctg tgaatgggat aaacattatt
      661 ttaaactcca atcttgcgta agagaaagtt gatatgtcat tagtaggata taatagtaac
      721 atcataataa ccatcgcgct gatatcgttg ctctctgcat gcaccagaga aaattcatta
      781 aaggagaatc aaaataaaag aagtggcgaa tcccaagcga tagtagatgc aagacagttc
      841 tttagcgaac acccggaata tatatcatca gcagaactgg agcaaaagct atatcgcgaa
      901 tttgcacagg ttactacagt tccagcttat aaagagatga gtatgtatca attgcttatc
      961 atttcacaag atcgtttaac tcattaaata aggatctaca cgctttttgc gtgtagattc
     1021 ttataaattt ctgactacga cagatgttta attttttaaa ttatcgtctt tattctcgat
     1081 ttttgtgtct tcaccctcaa tgaatttatc ttctgaagag agctgttcat cttgaacagg
     1141 ttgcccagca ttctccacat cttcaagcca cagcagaaga cgtaatattt catctatatt
     1201 ttcgaagcta acataatcgt agcgacgatg ggtagcataa ttttttcgtg ctaatttttt
     1261 atctgtgata attggtattc cgatttcctt tgcatatttc cttacgccta aggccacctc
     1321 attagttact cttacagaaa tcaatggaat aggtgaaaga tgtggcttaa agtatatccc
     1381 tattgcaatg tgagtagggt tggaaatcat caaacgacta ttactgacat cagacttcaa
     1441 ttgctcagaa agaatttcct gatgcgctct ctgcgtttag acttaatttc tgggtttcct
     1501 tcttgctcct tgtattctct tttaacttct tgtttatcca ttttcatatc tttcataaat
     1561 aaaaaatatt cagcgataaa atcaaaaatt aaga
//
LOCUS       DAZFJF010000348         1586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFJF010000348 DAZFJF010000000
VERSION     DAZFJF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.351"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..78)
                     /locus_tag="ACHKAU_005371"
     CDS             complement(<1..78)
                     /locus_tag="ACHKAU_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO5534211.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNT"
     gene            complement(94..828)
                     /locus_tag="ACHKAU_005372"
     CDS             complement(94..828)
                     /locus_tag="ACHKAU_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO5534212.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(825..1156)
                     /gene="sctI"
                     /locus_tag="ACHKAU_005373"
                     /pseudo
     CDS             complement(825..1156)
                     /gene="sctI"
                     /locus_tag="ACHKAU_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(1176..1416)
                     /locus_tag="ACHKAU_005374"
                     /pseudo
     CDS             complement(1176..1416)
                     /locus_tag="ACHKAU_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1430..>1586)
                     /locus_tag="ACHKAU_005375"
     CDS             complement(1430..>1586)
                     /locus_tag="ACHKAU_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO5534213.1"
                     /translation="NNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQG
                     SNWTFQE"
BASE COUNT          472 a          316 c          261 g          537 t
ORIGIN      
        1 tgtattattc aagcttactc gttgaggatg aatataacta attggatcat atataatctt
       61 tcttagggca agattcataa cgctctcata tgtctactta attttcaacc tgactaaatt
      121 agttagaatg gccctatact tccataacag ccagcaagtc gctacggata ttaatgcaag
      181 taagatagaa accggcatag ccttatcata agcaaaaaca ggttcgctaa tttcatatgt
      241 tggtgcttgc tcaataatgt ctcttcgttt tgacaataca acagaaatat tttcatattg
      301 tacgcttgca gagctattaa caataaatct cttgatatca tttattttta tttctgggtt
      361 gatatctttt tcatatactg caagtacaga aatatgaatt ggtaaagcag ttttaccact
      421 atcgccatta tcaacatcgt aactaacatg tactctcgaa gaaataatgc catccataat
      481 tttgagagat tgctctaacc gctgctcaat agcagaatat agccttgctt tttccgctcg
      541 tggagatgaa acgagtgcat ctgcagggaa catctgcgat atttgaatat caggcttacc
      601 cggtagattg tagattttta gccaatccac cgcagaagca aaatccgttg gttcgacaaa
      661 tattgaaaat cctgttttgc cttgatcctt cttttcagca ttaatattat gtctttgtaa
      721 aacagcaagg acatcattag cttgctgttg atcaagatgg ttcaataatt cctgctgctt
      781 gcagccgcac aacagcagga taaacaataa taatgatata tatttcatga gcgggtcaac
      841 gtctcaactg cagcgacaat ctttcttgct aatgttccac caatgaaacc tcgacattat
      901 aattaagcac atgatcctgc aagactaata atttttcagg agttaacccc tctttatcag
      961 accttaatgc ttgtaacgca ttttgctgcc aattttcagt ttgtacagca tagttggcaa
     1021 aagcggagat cattttatca tccgggttgt caagattgag aacccggtca tttaaatcaa
     1081 tcactttcgc ttgctgtatt tcatgagcag ttgattgctc agctctatca ataggtggca
     1141 tattgcttac taacataata aaatccttat ctagtttagc ggaagttttg tatgattgct
     1201 gaatcaagat ctttatacgc ttttacaacg ttagattgcg catttcgata taatgtatac
     1261 tcagctaatg cactttgata ttcggcaaga aatttcgtgt cggagggatt ggttggctaa
     1321 atcctccaac gctttttcaa cttgctggtt cagatcatca acgccctgat caaattgatt
     1381 gctgatatcc ataatataac cattccaatc tgccataagt cctcctcaat tattcctgaa
     1441 atgtccagtt gctaccttga gacttctcga atcggttatc tccgtagcta aatgaactat
     1501 tatttgtgtc ctgatttttc aaattcacat taaatcttac atattgttta ccccattgtt
     1561 tataatattc gtcaacatag ttatta
//
LOCUS       DAZFJF010000349         1585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFJF010000349 DAZFJF010000000
VERSION     DAZFJF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.352"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..683
                     /locus_tag="ACHKAU_005376"
                     /pseudo
     CDS             1..683
                     /locus_tag="ACHKAU_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(694..765)
                     /locus_tag="ACHKAU_005377"
                     /pseudo
     CDS             complement(694..765)
                     /locus_tag="ACHKAU_005377"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            828..974
                     /locus_tag="ACHKAU_005378"
                     /pseudo
     CDS             828..974
                     /locus_tag="ACHKAU_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1001..1105
                     /locus_tag="ACHKAU_005379"
                     /pseudo
     CDS             1001..1105
                     /locus_tag="ACHKAU_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          468 a          334 c          360 g          423 t
ORIGIN      
        1 atcagatgtc cttcctgctc cgctactgaa ggcgtggtgc gtaacggcaa aagcactgcc
       61 ggacatcagc gctatctctg ctctccttgc cgtaaaacat ggcaactaca gttcacttac
      121 accgcctctc agcccggtaa gcaccagaaa atcattgata tggccatgaa tggcgtcgga
      181 tgtcgcgcca gtgcacgcat tatgggcgtt ggcctcaaca cggttttacg tcacttaaaa
      241 aaatcaggcc gcagtcggta acctcgcgca tacaaccggg cagtgatgtg attgtctgcg
      301 ctgaaatgga cgaacagtgg ggctacgtcg gtgctaaatc acgtcagcgc tggctgtttt
      361 acgcgtatga caggatacgg aggacggttg tggcgcacgt cttcggtgaa cgcactctgg
      421 ccacactgga gcgtcttctg agcctgctgt cggcctttga ggtcgtggta tagatgacgg
      481 atggctggcc gctgtatgaa tcacgcctga agggaaagct gcacgttatc agcaagcgtt
      541 acactcagcg cattgagcga cataatctga atctgagaca acatctggca aggctgggac
      601 ggaagttact gtcgttctca aaatcggtgg agttgcatga caaggtcatc gggcattatc
      661 tgaacataaa acactatcag taagttggag tcattacctc ctcccgaact caaagctatc
      721 atacagataa ttactggtta accctatata gggcatttta aagtgttgtg atcttaccca
      781 gcaatagtgg acacgcggct aagtgagtaa actctcagtc agaggtgatt cacaagacaa
      841 aacagtttca accagtaaaa aaacgtaaac accattcgca tgaatttcgt agttaagatc
      901 tgaagcttgc agaatgcatc ggtgttactg ccggagttcg tgaacttagt ctgcatgaat
      961 caccacagct ttacatctgt cgcagtaaac agcaaaatca gcaaacttac ttaattatat
     1021 cgagtgcgat tacaatcgct ggcgatggta cagttggtgt ggcggtctca gtccggaaca
     1081 atttgaaaat cagaatctca cttaaaacgg catccatttt acaaaagatg gccgtctgtg
     1141 tatttgtaat gaataccttc tacatgttta gagtgcataa tccacgacaa ggcgatatcc
     1201 taaaaattag ctagcaggca actttgatgg catagataat gtttttagta aaaaagagtt
     1261 gactgaatat aatacagcct cttcctacat aagaaggaat gtgctcacaa aaaaaaacca
     1321 ttagtctgct tcctgttctt acaataatca gtattatgta ttttatttat atagcaaaat
     1381 atgcttgtta cacctgtcat aattaggcat ggtaaaatta ttgactgaac cctgggggag
     1441 aaagatgcac agtgattatt ataatatggt ttttggtgaa aaattggcca atattttata
     1501 tgaagctaac tctcaatttt tttacgagag aaatgtcata gaagaggcag tcaacgcttt
     1561 attctgtgag cgtgaaataa taaac
//
LOCUS       DAZFJF010000350         1583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFJF010000350 DAZFJF010000000
VERSION     DAZFJF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.353"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..459)
                     /gene="sctT"
                     /locus_tag="ACHKAU_005380"
     CDS             complement(<1..459)
                     /gene="sctT"
                     /locus_tag="ACHKAU_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO5534214.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQS"
     gene            complement(461..721)
                     /locus_tag="ACHKAU_005381"
     CDS             complement(461..721)
                     /locus_tag="ACHKAU_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5534215.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(731..1396)
                     /locus_tag="ACHKAU_005382"
     CDS             complement(731..1396)
                     /locus_tag="ACHKAU_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5534216.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(1386..1583)
                     /locus_tag="ACHKAU_005383"
                     /pseudo
     CDS             complement(1386..1583)
                     /locus_tag="ACHKAU_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
BASE COUNT          548 a          345 c          242 g          448 t
ORIGIN      
        1 actttgccaa agagtttcca gaataaagtt cattccacct ttagtaagat aaaccgcagc
       61 agagaaaagg ttaaataatc ttgctaattc cgaggtatcg acacctgtag ccggatccaa
      121 agtactactt aatgttgcac cgcgttggtt atcgataaag ctacctatag caaggaaaat
      181 ccaaaacggg gatgctaata agcatgcaat aaacagccct atgataattt ctctggcagc
      241 gaacaaagca atatcgattt catttaagtt caataattca tagtggtaat gctgaactaa
      301 tgcacatgat accactatta ttattgggtg acgaactact gttggtatat tcccactggc
      361 gaaaaatggt aacaaataga aagttggtgc caatcggcaa aaaccgagcg ctgtagcagc
      421 cagcagacta tgcaactgat agagtatcgc ctcccccatg ctatcctctc gcgaatgcta
      481 aattcagcat ttcgatacca aatgaatata atttttcacc ataccacccg ctcattaaaa
      541 aaaagcaaat acttacgcac aataatttca cgccaaatgg caacgtctgt tcttgcaact
      601 gcgttaccgt ttgaaaaagc cctaccaata aaccaacaaa tgtcgcaact gcaataggtc
      661 ctgcagacat aactaaaatc agatacagcg ccctattccc cgcaaaaaca atatcgtcca
      721 tatttttttc tcagggatta atggataggt caaaatattg taaaatgagc ccttttgata
      781 acattgtcca accgtccatc gcgacaaaca aaattaattt tatcggtgta gatatggtta
      841 ccggactcat catcatcata cctaacgtta acagaacgct ggatataacc agatccacta
      901 cgacaaaggg taaatagata taaaaaccta taataaatgc actttttatc tcactcaatg
      961 catatgcggg cagtaacgag aaaattgaaa tattatcatc atcgattatc tcttcattgt
     1021 cttcagaact attcaccttc tgaatctttt caaaaaagct aaccaactca ggttcagaat
     1081 attttatcaa atatgattta taaccactca tgcctgtctc aacaaaatta acaaccgatg
     1141 ctacattatt gaatgaaaga ttctcattct gactgttatt atatatttcc ttcccaaccg
     1201 gcatcattac gaacattgac aatagcaaag caaccccatt aagtgtcata tttgatggca
     1261 cttgctgaag tcccagcgcg tttcggacaa taacaaaaac aatcgagaat ttaataaaac
     1321 atgtcccgga tgctattata aacggcaata gtgtaaataa tgataatatc gctatcagcg
     1381 aaatgctatt tgacattatt attccctgat aaccaagaat gaatttctac gcccaattta
     1441 tcatccactt caacaagttc gccatagcca gttagcgcac catttacacg tatctctata
     1501 ttttctcaga ttcaggaagc agagatatta ttctttttgc acagagatcg tctatttcat
     1561 aaagattaat tattttctta cca
//
LOCUS       DAZFJF010000351         1582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFJF010000351 DAZFJF010000000
VERSION     DAZFJF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.354"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(368..>1582)
                     /gene="nleL"
                     /locus_tag="ACHKAU_005384"
     CDS             complement(368..>1582)
                     /gene="nleL"
                     /locus_tag="ACHKAU_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO5534217.1"
                     /translation="KVRAAEELIKKISLDELAAFRPYVKMSLADSFSIHPYLNNANIQ
                     QWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVMN
                     GSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLS
                     SQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLFSESFPIFS
                     IPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSDYKMIADDQQRKLACVWNPF
                     LDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEIL
                     RRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFP
                     EIFSLYYPVAWR"
BASE COUNT          570 a          342 c          214 g          456 t
ORIGIN      
        1 acaaaatcaa ttatcacatt caaaaaaaaa tactgacaac aaagatactc ttcagacaaa
       61 gttgtcatat gtaaattttc ccttaaaacg ttgctcaaaa aaaatataaa aatcatatgc
      121 cctaatcaac atcctcaagc actacgatat atgcagtatc ctattcatat attaccacca
      181 tatcagacaa atcgaatcac aacgagttct atcgtactct ttcatcttcg acactgtaaa
      241 ttttaagaac attcggctca tgccactaaa ccaagacata ataacaacgg agcaataaat
      301 gttcccactg aacatgtgca aacctaaaaa gacagtgtta caataatatt catccatggt
      361 ctctaaatca acgccacgca acaggataat acaatgaaaa tatttcagga aaagattctt
      421 ttgcatgctc cgttagcata tcagtcagca cagcggtaca agtaaaaaca ttatttcttc
      481 cttgtagcct gtctaaaata tcattgtaat aataaactga gccaaatacc tgaggatctg
      541 ttttataagc ttgttcaatc aatccatttg catatctccg tagaatctca ggagaatcat
      601 actctgtacc aaacatatca ctcgatgagt atttacagaa aaccccccct aaacaaaata
      661 atatttcagc ctgttttctt agtggcatat ctttcaatac atggctcccc ataatcatat
      721 ctacatgctg agcgttcagt tcccaaccat caagaaaggg attccagaca caggcaagtt
      781 tcctttgctg atcatcagca atcattttat aatctgattt attggaatta agtgcctcaa
      841 taaatctctc tttgagttca ttatcagaaa tgagtatatc cagaatacca gaaacgaaat
      901 tctgggaaaa agctttatga taaggaatac tgaaaattgg aaatgattca gaaaaaagat
      961 tgctcaatga atacccaaat gtattttgaa cttctccatc cttatataaa aaaaaggaca
     1021 tccagttagt tgatgtatta ggagataaca tatcaaccat atgactcaaa gaacacatca
     1081 ttgccatacc atcatcctgt gatgataaca aaacccagtt ataagccaaa tcggaatcat
     1141 catcccagtc aggcttccca gagccatcgc acaatccaaa atcactctgc tgtgtgacag
     1201 gataaacttt ttcatttttt aaatatactt catagagttc cctaaatcgt tcctgcattc
     1261 catcacgccg tgaaccattc attacaattt gtataaagga actattataa gataccatcg
     1321 cacctggatg tcgctcaaaa tacatccctg cctgcaataa actaccattc tccatataca
     1381 tcattattga attattaaac caagacatta tagtatcaaa aaagtcatca catataggct
     1441 cgagccattg ctgtatattt gcgttgttca aataaggatg aatactaaat gaatcagcca
     1501 aagacatctt aacatagggc cggaacgccg ctaattcgtc aagagatatt tttttgataa
     1561 gttcttcagc ggcccttact tt
//
LOCUS       DAZFJF010000352         1567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFJF010000352 DAZFJF010000000
VERSION     DAZFJF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.355"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..641)
                     /locus_tag="ACHKAU_005385"
     CDS             complement(<1..641)
                     /locus_tag="ACHKAU_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5534218.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIART"
     gene            910..1026
                     /locus_tag="ACHKAU_005386"
                     /pseudo
     CDS             910..1026
                     /locus_tag="ACHKAU_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1131..1207)
                     /locus_tag="ACHKAU_005387"
     tRNA            complement(1131..1207)
                     /locus_tag="ACHKAU_005387"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1171..1173),aa:Arg,seq:tct)
     gene            complement(1221..1297)
                     /locus_tag="ACHKAU_005388"
     tRNA            complement(1221..1297)
                     /locus_tag="ACHKAU_005388"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1261..1263),aa:Arg,seq:tcg)
     gene            complement(1305..1380)
                     /locus_tag="ACHKAU_005389"
     tRNA            complement(1305..1380)
                     /locus_tag="ACHKAU_005389"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1345..1347),aa:Ile,seq:cat)
     gene            complement(1409..>1567)
                     /locus_tag="ACHKAU_005390"
     CDS             complement(1409..>1567)
                     /locus_tag="ACHKAU_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5534219.1"
                     /translation="RVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRY
                     VSRQGLDY"
BASE COUNT          384 a          436 c          398 g          349 t
ORIGIN      
        1 gtgcgcgcaa tcaggtcctg atataacggt ttacccacac cccagcgcgc cgaatcctgg
       61 ctggcccccg tgtccgcact gaatgtcccc tccgcgccct gggtaaatgc cgaaccacca
      121 cgacagcatg gtaccagcag gatccccgcg ttattcggga tatacggaag cagttttttg
      181 gcaatatgta agccctggcc gacacagccg tactgccctt tgctcaggtc tgccttcgga
      241 tgattcagcg tactcatatc ctgcacatca tgcaggcagt ggtcagccgg aatgatgtcg
      301 ttatacgtac aactctctcc acccggagtt accgtgctgc ggcgcgccag ctgtttaatg
      361 cgcggatccg gagcatcgta agaatccggt aacggaagcc cttcaccgta ggccatgccg
      421 ttggactgtc cggcaagcac aaccacgtag aaccagtccg gctcagatga agggccgacc
      481 tgtggatctc cttcaatagc caccgcctgc atcagtgtgt acggcgtaat ggcaaccggt
      541 ccgccgtatg gctgccagcc ctctttcagt ttgtgtgtca gcttttccgc aaggtctgac
      601 ggcgacgccg ccctgacaac atcatagtgt ttaaatgcca tgaatcctcc cggccgggat
      661 aatattgtga gtaaaatgag gagcgggctg aagtccggaa gttacaggac aatggcagaa
      721 gagagacaac agcccgcaat acgaaaaagg ccgcgctatt gcgcagagtg attactgtcg
      781 gatattattc gccagctgaa atattacttc acgttttgtt gtttattcct tgccgcccgc
      841 gtctccctgc gcgggctttt tttgtccata agaaagcccc tccggagagg ggctggagag
      901 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      961 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
     1021 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
     1081 tgagacattt aaaaaaaagg cccgcaaaag cgagccaggg aaaataagtg tggcgcgttg
     1141 tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc ggctgagcta
     1201 acaacgcagg atacagataa tggaccgcct tcggggaccc gaactccgcg caaccagctt
     1261 cgaaagctgg cgctctttcc tgatgagcta atggcggtat gtgatggtgg cccttgctgg
     1321 atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc
     1381 gggtcaaaaa ataataatca gatgaaaatc aataatcaag cccttgcctg gatacatatc
     1441 tgtctggcgg gaagccataa tagcggtgaa atacagaaat aaagtaggac cagcttgaat
     1501 aaccgcattt ttctgctaca gcctgtccat atccatgccg ggaacataac atattcacag
     1561 caacacg
//
LOCUS       DAZFJF010000353         1566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFJF010000353 DAZFJF010000000
VERSION     DAZFJF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.356"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..654)
                     /locus_tag="ACHKAU_005391"
     CDS             complement(<1..654)
                     /locus_tag="ACHKAU_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO5534220.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
     gene            complement(818..913)
                     /gene="ymiC"
                     /locus_tag="ACHKAU_005392"
     CDS             complement(818..913)
                     /gene="ymiC"
                     /locus_tag="ACHKAU_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO5534221.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1027..1194)
                     /gene="yciX"
                     /locus_tag="ACHKAU_005393"
     CDS             complement(1027..1194)
                     /gene="yciX"
                     /locus_tag="ACHKAU_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO5534222.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1197..1337)
                     /locus_tag="ACHKAU_005394"
     CDS             complement(1197..1337)
                     /locus_tag="ACHKAU_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO5534223.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          431 a          370 c          359 g          406 t
ORIGIN      
        1 gatcatagtg gtgtgcgagt cagtaccaac gagtgtatcc ggataagcaa tccattcacc
       61 gtcctgcaat tcactccaca ctgctttgcc gagatattcg aggttaacct gatggcaaat
      121 gcctgtgcct ggcggcacga cgctaaaccg actgaacgct tgctttcccc atttcaggaa
      181 cacataacgt tcgtggttgc gctccatttc caggcgtacg ttttcttcaa atgcctcatc
      241 atcaccaaaa cgatcgacgg tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg
      301 gttaaccttt gcagtatcgc cgccgaggcg tttaaccgct tcgcgcattg ccgccagatc
      361 aacaacggca ggtacgccgg taaagtcctg catcagcacc cttgccgggc ggtaggcaat
      421 ttcacggtca gcatgggcat ttttcagcca tcctgccagc gcgtggatat cctcttcggt
      481 aaccgagtta ccatcctgcc agcgcagcag gttttcgagc aaaactttga gtgacttggg
      541 tagacgggtg atatcgccca gtgatttagc agcaagcggc aggctgtagt agtggtaagt
      601 tttatctttg gcctgcaacg tgtccttact ggcttctcgt agggttgacg acatagctcc
      661 tccttaatga cagggttgcg aagccctgat tctcttcagg gtgaatatta aagataacac
      721 aaacatcgcg taacgatttg ataacaaccc aaattgataa aagaggagaa accttgtggc
      781 aaagcaaatg acaaccccgc cgcagcgggg tcaaggatca gtgagtaatg atccagagga
      841 gttcggccca gaagagcatc gacagagaaa acgcgcccat ccaggaccaa tatttcatat
      901 ttgtgttgat cataggctac acccttaacg gttattattt tgtgttgaga taactcaagc
      961 tgttattgct gcggtgtatt aaaagccctt taattcatta aaaatgggta ataagaatga
     1021 tgataattat tgtttcggct gatcatcttc agcaaacagt tctataaagg cttgctgctg
     1081 cgatgttagt tgccagtcgt ctggaataac gacggaacat tcccgtttcg tttctgtttc
     1141 attgtcacta tgctggcata atggtgacga ctccagttgc tcctgaccga ccatagttat
     1201 ccccacactt tccaaatcag cagtgcgaca accacccaga aaagtgcaga accaagaaaa
     1261 accgccagcc aggctttacg ttttaattca ggatcgcggc ggggttcctg atttccagaa
     1321 ggcattgcta acctcatata atcgccatca cttatcagca agacgttgaa cgattggtgt
     1381 aattaatcat acgatgagac aaatcctaaa gaaactttag tcaaaaatcc agcgaattta
     1441 cgaatcgtct ccagtgaata attcaatctt ttgacctgaa atagatattt gaaaactgga
     1501 gtttgcgagg cgggtaatta gacactactt tacaggtaat ttaccctata aatatgaggg
     1561 ataaaa
//
LOCUS       DAZFJF010000354         1563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFJF010000354 DAZFJF010000000
VERSION     DAZFJF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.357"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(192..845)
                     /gene="ribB"
                     /locus_tag="ACHKAU_005395"
     CDS             complement(192..845)
                     /gene="ribB"
                     /locus_tag="ACHKAU_005395"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO5534224.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(949..1097)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1219..1509
                     /gene="ubiK"
                     /locus_tag="ACHKAU_005396"
     CDS             1219..1509
                     /gene="ubiK"
                     /locus_tag="ACHKAU_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO5534225.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          446 a          398 c          352 g          367 t
ORIGIN      
        1 tattcatatg gtcgcataat aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa
       61 tgcctgatat taatgaaatt ttctcataga aagagaagcg gaattaacat cttgcatagc
      121 agggcaaaat aaaaaccgaa gccagctaca acttcggttt cttgattaag gcagtaatta
      181 agcagcggtt ttcagctggc tttacgctca tgtgcctgac ggtatgccac caggtcttca
      241 atagtcacga gcgccatatt gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc
      301 atcgtgccat cgtcattagt caactcacac agcacaccag ccggtttaaa gcctgccagc
      361 gtcatcaaat caatagtcgc ttcagtatga ccgccacgcg tcagtacacc acctgcctgc
      421 gcgcgaagtg ggaaaacgtg gcccgggcga ttcagatcag aaggttttgc gccatcggca
      481 atcgctgcgc gaacggtcgt aattcgatca gcggcagaaa caccggtagt cacaccttca
      541 gccgcttcaa tggtcacggt aaaaccggtg ccataggcgc tggtgttatt ttctaccatc
      601 attggcagat caagttgttt acggcgatct tcagtgatgc acaggcaaac aataccgcta
      661 ccgtggcgaa tggtcagcgc catctgctca acggtcatgg tttctgccgg gaagatcata
      721 tcgccttcgt tttcacggtc ttcatcatca agcaccatta caccgcgtcc ttcacgcagc
      781 gcagccagtg cattttcaac acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga
      841 ttcatggtaa aaaaacctca ctaaaattat ggttaccaga atcagggcag tcttaggagt
      901 ggcggcatat agccaaaata acgtgagcgg gtccatgccc gacagaatcg ttactctctc
      961 ccatccggac tctaaccgtc ggccccggaa ttacaccgga tctgctgtcc tttgagttcg
     1021 cacccaaagc gctcgcgggc tttcaactga gttgatttac cgccggtggg gaatttcgcc
     1081 ccgccctgag aataagcgga ttcactataa cgctaatgat tagcggcagc aacgcatagc
     1141 ttcacataat tctggtttat gacttaccct tatcgcacta caatggcact caacacctat
     1201 cagtacaggg aaactacaat gattgacccg aaaaaaattg agcaaatcgc tcgccaggtt
     1261 cacgaatcaa tgcctaaagg aatcagggag ttcggggaag atgtggagaa aaaaatccgc
     1321 caaaccctac aagctcagct gacgcgcctc gaccttgtaa gccgtgaaga gttcgacgtc
     1381 caaacgcaag tcctgttacg gactcgtgag aaactggcgc tgcttgaaca aagaatgagc
     1441 gaactggaaa atcgcagtac tgagattaaa aagcagccag atccagagac tctcccccca
     1501 acgctgtaaa catttgtctt taatgcactg taaattatga attaatcctc taatttacag
     1561 tgc
//
LOCUS       DAZFJF010000355         1561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFJF010000355 DAZFJF010000000
VERSION     DAZFJF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.358"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..261
                     /locus_tag="ACHKAU_005397"
     CDS             <1..261
                     /locus_tag="ACHKAU_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO5534226.1"
                     /translation="ENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLI
                     AAGLESNLTVYTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(403..1527)
                     /locus_tag="ACHKAU_005398"
     CDS             complement(403..1527)
                     /locus_tag="ACHKAU_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO5534227.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVLGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          465 a          357 c          289 g          450 t
ORIGIN      
        1 gagaataata cctcctgggt aagtgagcag atactttcag cgcctccgaa agatgtaaat
       61 attcgacttg gagtcggttc tttagaaggt acaaccgtct cacatgttca gcgattgcat
      121 cattcgttaa tcgcagctgg tttggaaagt aacctcactg tctataccgg tggtcacgat
      181 tatgcctggt ggcgcggagc aattattgat gaattagcaa attataattg caggaaggta
      241 tcaaataata actctgtgta aaatatcgtt ttatcgtcag ataactcttt ctgcaatatt
      301 attatgggag actatctcat ttatttagtg atgtccttca tttatgtgat gaaggacatt
      361 atattactca taacatttct cgttgaaaat ttcagcaata cgctacaatt gtttcccgtt
      421 gttttttaat tttctcatca aaacctctgc aatctctgca ggactgggtt gttcagccat
      481 ttcagcggct acctgatctg aacagaagcg tagtgaatca tcataaagaa ggcgatttac
      541 caaatcactg gtcagtccat gcttgtccgg aataatccca cacccacgct tcgcaacaat
      601 ttctgcatta acagagcgat ctgcaccttc gccaaacaca atctgtggta ttccactata
      661 cagcgctgtc agggtatttc ctgcgccacc gtgatgaata aatccatcag ctccattaag
      721 gaatacaccc ataggtatcc agtcaaccag acgtacattt gatggtagtt ttcgtaaacc
      781 agtacgagca tttattgcaa gttgcaaaat gatatcagca tcaacttcat ttgcagaatc
      841 cataacccat gaaatcagct ccagaccatc aaccattggt tttacagtgc caaggctgat
      901 aagtaaacgt ttccgatcag aatccctgtc ccaccatgtt tcctttacag cacctccgtt
      961 ataaggaata tatctcattg agataaccgg ttctccagca tttttaagaa tgctcatact
     1021 tgggggagca acatcaatcc atgctaaatc acatagtgga ccgctgactc catgccgcct
     1081 gtaagcattg cttaaagaac ggtttaacat ctgaatatgg gcagatgtat gcgcgaatcc
     1141 aacagccagc atcactgaag gaattctata tttagcagca accaatgggc ctgccggatc
     1201 aagaggggga tagactatta aatctggcct ccattttcct gcaaaatcga tgaggttatc
     1261 tgccatttca tcgctaaaaa aagagaaatg accaataata ttactttttt tcctcaactc
     1321 ttcctgatgg cgatatccag cctctgaatc taaacctaga actgcatcaa aaacaaccag
     1381 tccagcttct gctgctttat tcgcgaattt gccagcgcta ctaattacta catcatgtcc
     1441 aattacacga aaggcctgag ccagagaaat caatgggtat aacaaaccgt acagcggtgg
     1501 gccaataaaa agaatacgtt ttcgcattaa aaaagtccgt cttctgctta cctgtttatt
     1561 t
//
LOCUS       DAZFJF010000356         1559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFJF010000356 DAZFJF010000000
VERSION     DAZFJF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.359"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            224..385
                     /locus_tag="ACHKAU_005399"
                     /pseudo
     CDS             224..385
                     /locus_tag="ACHKAU_005399"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            369..434
                     /locus_tag="ACHKAU_005400"
     CDS             369..434
                     /locus_tag="ACHKAU_005400"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO5534228.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            550..807
                     /locus_tag="ACHKAU_005401"
     CDS             550..807
                     /locus_tag="ACHKAU_005401"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5534229.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(854..1423)
                     /gene="nleF"
                     /locus_tag="ACHKAU_005402"
     CDS             complement(854..1423)
                     /gene="nleF"
                     /locus_tag="ACHKAU_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO5534230.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
     gene            complement(1489..>1559)
                     /locus_tag="ACHKAU_005403"
     CDS             complement(1489..>1559)
                     /locus_tag="ACHKAU_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534231.1"
                     /translation="HQVMQSYHGGKLDLISVVLSKI"
BASE COUNT          466 a          357 c          306 g          430 t
ORIGIN      
        1 taacatcaat agattatgat ggcttcaatc taaataatag gctatataat actgcaagac
       61 acaacacatg ctgtcactat gagtcaacta tttagatgat attagtgacc tgtttcagag
      121 cattagcgca aggcgattct ttgtcttctt gcactaattt tttatcataa aaatgttcct
      181 agcactgggc atcaatatcg caggtcagaa agagctcctg gggatgcggc tggccgaaaa
      241 tgaaggggcg aatttctggt tcaatgtgct gactgaactg aaaaaccgcg gtctgaacga
      301 tatcctcatc gcctgtgtgt atggcctgaa agaattcccg gaggcccgca tccagttatg
      361 catcgtgcat atggtgcgca acagcatgcg cttcgtgtca tggaaggaat acaaagccgt
      421 cactcgcgac ctgaaagcga ttagcctccc acagaagagg caggccagca ggcactggaa
      481 gcgtttgctg cggcctggga ctgccgctat ccgcagataa gccggtgcta gctgtcaaac
      541 tggactaact tggcgacgtt tttcgcttat ccggcagata tccgcaaagt gatctacaca
      601 acgaacgcca tcgagtcgct gaacagtgtg atccggcatg ccatcaggaa acgcaaggtg
      661 ttcccgacgg acgagtcagt aaaaaaagtg gtgtggctgg caatccaggc cgcgtcacag
      721 aaatggacaa tgccgttaag ggactggcga atggcaatga gccgctttat tatcgagttt
      781 ggtgaccgtc ttgacggtca cttttaagaa aaggcattta cacagaatcc taaacaggct
      841 cacaggcctc taatcatcca cattgtaaag atcctttgtt gtaagtaaga tctggtaccc
      901 taataatatc aaaaggatta aaatcatcac tgcatccttc cacgaggcat ttcattgctc
      961 gtagatcacc atatagctct gaagagtgat gcggacatag cagattataa actttacatt
     1021 tcgaatcttc tttgccttgt ctactttcct caatttttac tttcaatgaa tgcagaaaaa
     1081 aaagatccct gatataacta tccctgtcct ctatcgtcat tcccgccctc agcgctccga
     1141 caacctcatc agtattttca tttctcacct caaatacact atttctctta tccatcagcc
     1201 cacttacaaa agcacttagc tcttgaaaaa tttcctcaag ctcattatta tttctggcac
     1261 atgaatatat agagaccata acatcaagtt tatcttgtaa ttcaggagaa agaaagtgat
     1321 tatgattaag ctcacttact gattccggag tcgaagggtt acctcttcct gatacataca
     1381 tccatgaata aagatttgct gaagaaccac ttgttggtaa catatcaaaa cccccttaac
     1441 aaataaatcc ataaatatta ttgcaactaa tatatctgga taaaaatatt atatcttact
     1501 taatactaca ctaataagat ccagctttcc tccgtgataa gattgcatga cttgatgtt
//
LOCUS       DAZFJF010000357         1558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFJF010000357 DAZFJF010000000
VERSION     DAZFJF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.360"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..187)
                     /gene="dicB"
                     /locus_tag="ACHKAU_005404"
     CDS             complement(<1..187)
                     /gene="dicB"
                     /locus_tag="ACHKAU_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5534232.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            complement(756..974)
                     /gene="ydfC"
                     /locus_tag="ACHKAU_005405"
     CDS             complement(756..974)
                     /gene="ydfC"
                     /locus_tag="ACHKAU_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5534233.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(1046..1345)
                     /locus_tag="ACHKAU_005406"
     CDS             complement(1046..1345)
                     /locus_tag="ACHKAU_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534234.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
BASE COUNT          381 a          404 c          350 g          423 t
ORIGIN      
        1 attgacgttc tccgaaaata ccaagttcaa gaagagcaat tcgggaaagt atggaattat
       61 cattgagcag ataaggctca tatttcctca tattaatggc atcttcagta aactcccggt
      121 tactgagcag aacaccaata tcaaaacaac cttcagacgt attaacgttt ggtaataacg
      181 tttccattat cgcgtcctca acaatgaatt ttgtgatgcg gtgcctggtg cctccaggtg
      241 acgttaacca gttaacaatt aacgccggat acagagaatc cacccataac actgtttttg
      301 gttttaactg ttccgcgtgc gctgagccgc attcaccgca tcacaaaatt cactttaaaa
      361 agggcggcag agcagccacg gagtaaaact gataccgcca aacgtcacca gaaaattgat
      421 aacagagggc gttgcagcgg ggttgtcact taagcgtatg gtcaacctga caacccggtg
      481 tcctcaactg gggaaggaat aaccccgcca tacttaccgc cgcgccattt cgcggattgc
      541 cacaaccgga agcgcacggt cgacgaaaat ttaacgacag gctatctatg aaccagctac
      601 ctcgccgtgc gctttcgcgt tatggtctga cttttcaggg aaatatcctt tcagtaaact
      661 gtcagtgccg gatgctcacc cgtgtccggc gcacgcactc cacctcaccc gtggagaact
      721 ccttaattac caaccttagc ttcgttggtt agctattaac gcgggtatgt aatcattctg
      781 gcaatgctta atgccgctgc tttttccaga ttggtgatat cctgctccag agcggacaga
      841 ttttcagcct gcttagccct ggcttcattg gcccatttca ggtcctgcgc tgccttaatt
      901 ttctggtgca tccactcata aagttcatca tcggtatagt ctggcgcgat gatgacgggt
      961 tctcgtttct gcatgtcggc tccttgtggt tagcgctgcc tgcttttaac cacgtcaggc
     1021 gaggtggtat cctctgaggg gtctgttact cgagaggaaa ttggttatga atacaatcaa
     1081 gttttcttgc ccagaatgtg gtggcgaagt ctttgacaca tcctttaaac cgcatggctc
     1141 tgacagtttc gcgggagcca tctgcaaaaa ttgtggtcac cttgtaactg aagatgagtc
     1201 ctcgcagttt gatgacgaaa tcgttgacaa tatcttcggt gcactcacca gagactttct
     1261 gaagtaaagg cgcataccgc ttagttaccg ctctgataat tcttacctgt ccggcaatgg
     1321 cgctgatatc aatataaagc gccatcgctg tttctttgct gatccctgga tgccttccat
     1381 tctgatgttt gacttcgccc actgagaaat cctctgtttc cccttaacgc cggggtagcg
     1441 gaacaaaaaa cctgctgcat agttaaacgt aatccccgcc gtcatgttca tacgcctcag
     1501 gctggctact taacccctga ccactgccgg gtaactcgaa gtattgccct gcgttctg
//
LOCUS       DAZFJF010000358         1544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFJF010000358 DAZFJF010000000
VERSION     DAZFJF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.361"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..356)
                     /locus_tag="ACHKAU_005407"
     CDS             complement(<1..356)
                     /locus_tag="ACHKAU_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5534235.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
     gene            484..1344
                     /locus_tag="ACHKAU_005408"
     CDS             484..1344
                     /locus_tag="ACHKAU_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5534236.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
BASE COUNT          420 a          303 c          405 g          416 t
ORIGIN      
        1 ggaaccagac ggacttcatc ttttggcggg cgtccgcgtc gcgcgcgcac tttttcggac
       61 aggatccgca caaacggtct tacaccatcc agatcaaatg attccggtgt cagcatccgg
      121 gcttcgccaa tacgcattcc ggtattccag agggtggcga acagcatatg gtgacgctga
      181 tccggcatat agaaaagaag ggcactcact tccggggcca gcaggtaggc cggtgtggcc
      241 cctgtggaag tggccattct tctcagggag agcgctgttg caaaatccac ccccggcgca
      301 atgggtaaca gggagacgcg ttctggtgaa ttctgcaggg gaatgacatt gttcatgagt
      361 tatttttcac gtagcacacg cactgtttat ggatactgat tgcgctgggc attctggtat
      421 tgtgactggt atgatgctgg cgctgattct gatgattaat aaacaacata acacaggata
      481 tttatgaaaa ttacggatca caaactttct gaaggtattg ctctgacatt tcgtgttcct
      541 gaaggaaata tgaaacatcc tttgattatc ttatgccatg gattttgcgg tatccggaat
      601 gttctgctcc cttgctttgc gaatgctttc acagaagcag ggttcgcaac gattactttt
      661 gattatcgtg gattcggtga gagtgaagga gaacggggcc gtctggttcc agcaatgcag
      721 atcgaagata ttatttcagt tatcaattgg gcagaaaagc aggcatgtat tgataatcag
      781 cgtatcgggt tatggggaac atcgctgggt ggttgtcatg tgttcagtgc agctgcgcaa
      841 gatcaaagag tgaaatgtat tgtcagccag ctggcttttg ctgatggtga tgctctgatt
      901 accggagaga tgaatgaatt ggagagagct tcttttctgt ccacactgaa taaaatggca
      961 gagaaaaaga aaaacacagg gaaggagatg tttgtggggg ttacacgggt actaaatgat
     1021 gatgagtcga aggtattctt cgagaaagtc aaagctcagc atcccgaaat ggatataaaa
     1081 atacctttcc ttactgtcat ggaaacgttg caatataaac cggccgagag cgcagccaga
     1141 gtccagtgtc cagtattggt tgttattgct ggccaggata gtgttaatcc accggaacag
     1201 ggaagagcat tatatgatgc tgttgcttct ggtactaaag aattatatga ggaagctgac
     1261 gcatgccatt atgatattta tgagggcaca ttttttgagc gggtagcagc tgtgcaaaca
     1321 caatggttta aaaaatatat ttaatagtga gcctgtgttt accattctgt gtaaatactt
     1381 tttcttaaaa gtgaccgtca aggcggtcac taaactttat aataaagagg catcgttcat
     1441 ttccgggagg cagactggat tgccagcccc ccacttttta ccaagctgca ggtatggggg
     1501 acacggacac tggtgttcac agaaaaaaat aaccatctct gaaa
//
LOCUS       DAZFJF010000359         1538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFJF010000359 DAZFJF010000000
VERSION     DAZFJF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.362"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            215..1426
                     /gene="bluF"
                     /locus_tag="ACHKAU_005409"
     CDS             215..1426
                     /gene="bluF"
                     /locus_tag="ACHKAU_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO5534237.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          455 a          315 c          311 g          457 t
ORIGIN      
        1 ttagtgctaa tgcatataaa acaatgtgtt atgataaaac atattgcatc atttaactga
       61 caatctatac aagatttttc gccaactagt gttcaacttt ttccattttt tatcaatgta
      121 aagaaacggt tatgttttgg tattaaaaga aagacacaag aagatatgtc tgttaccgtc
      181 ttactctcgc ctcacccatt accctggatt gttaatgctt accaccctta tttatcgtag
      241 ccatatacgt gacgacgaac ctgtcaaaaa aatcgaagaa atggtttcga tagcaaatcg
      301 caggaacatg cagtctgacg taacagggat cttactgttt aatggttctc attttttcca
      361 gcttctggaa ggtccggaag aacaggttaa aatgatatat cgggctatat gccaggatcc
      421 acggcactat aatattgttg agctgctgtg cgattacgcg cctgctcgcc gttttggcaa
      481 agcgggaatg gaattatttg atttgcgcct gcacgagcga gatgacgttt tacaggccgt
      541 attcgacaaa ggcacatcaa aatttcagct aacttatgat gacagagcgc tacaattttt
      601 tcgtactttt gtccttgcaa ccgaacaatc aacctatttc gagatccctg ccgaagactc
      661 ctggcttttt atcgctgacg gatctgataa agaacttgat tcctgtgccc tttcaccaac
      721 tataaacgac cactttgcct ttcatcctat tgtcgatccc ttatcgcggc ggataatcgc
      781 ttttgaagcc attgtgcaaa aaaatgaaga tagcccatca gccatagcgg ttgggcagcg
      841 taaagacggg gaaatctaca cagcggatct caaaagtaag gcgcttgcat tcacgatggc
      901 acacgcactt gagctcggtg ataaaatgat ttcaatcaat ctattaccta tgaccctggt
      961 taacgaacct gacgcagtct cttttttact taatgaaata aaggccaatg ctctggtgcc
     1021 tgaacaaatc atcgttgaat ttactgaaag tgaagtcata tctcggtttg atgagtttgc
     1081 cgaagcgatt aaatcgctaa aggctgctgg tatcagtgta gcaattgatc attttggcgc
     1141 aggttttgct ggtttgttac tcctgtcacg cttccagcct gacagaatta aaatcagtca
     1201 ggaattgatt accaatgttc ataaaagtgg gccacggcag gcaattattc aggcgatcat
     1261 aaaatgctgt acatcacttg aaattcaagt cagtgctatg ggcgtggcaa caccagaaga
     1321 gtggatgtgg cttgaatccg caggaattga gatgtttcag ggagatctgt ttgctaaagc
     1381 taaattgaat ggtatccctt ccatagcgtg gccagagaaa aaataatttt cagcacattc
     1441 tttcacatga tttcagtaaa tcatcgtgaa aatgaatacg ttacgtttaa atttaatcca
     1501 cattaaaaat aatgtttcat ccaatttagc tgaaatct
//
LOCUS       DAZFJF010000360         1538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFJF010000360 DAZFJF010000000
VERSION     DAZFJF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.363"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(2..700)
                     /locus_tag="ACHKAU_005410"
     CDS             complement(2..700)
                     /locus_tag="ACHKAU_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5534238.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            complement(700..1029)
                     /locus_tag="ACHKAU_005411"
     CDS             complement(700..1029)
                     /locus_tag="ACHKAU_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534239.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1026..>1538)
                     /locus_tag="ACHKAU_005412"
     CDS             complement(1026..>1538)
                     /locus_tag="ACHKAU_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5534240.1"
                     /translation="VLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIEAA
                     AANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVG
                     GAGSPAQMRRTEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELRLQLR
                     DGGMLSGSGR"
BASE COUNT          319 a          558 c          348 g          313 t
ORIGIN      
        1 tttactgcga aagtttattg atggaaagga aaccgccaaa attgaccgcc atgccgcgca
       61 tctcacaccc gcgcatgcac ttgctgcatc tgtccttacg gatatccgtg gtggggttgt
      121 cgaactcatc cgccaccgca ggaccgttat acccgcattc atctccccgg taatcccaca
      181 tacaggtgtt cgccagcatg atgcgaccgg gaaacagcgc cccgtccgtc tcggtcggtg
      241 tggccagcac aaacgaggcc gtcatggccg tcagcgatga catctgctcc accacccacc
      301 ggtcagtcag ctcctgctcc gggtcggcct ccggattgcc tgccacaaag ttcaccgcat
      361 ccagaaaacg cgcatacacc cggcggcgga ccaccgtggc acccaccagg ctctgcaaat
      421 cctccgccat cccggtgaca agaccgaaca gattggacac cgtcagcgac gggcgggcac
      481 tgctgccctt tccgttcatc tcaaagccgc tgccctcaat cgggtacgcc tgatattcac
      541 gcccctgcca ggtcaccggc tccccttttt cattcagctc attgcagaaa aaataccgct
      601 caccgccctg cgccgtcagg tcgatttccc agagcaccac ccgcggtgac tgctctgatt
      661 taaccgactc gttcagactt tcttcgtgaa tatcctgcat cagttcacca cctgcttaaa
      721 ctccgcgctg aactcaacgc gcaacatccc gacccgcgca gaccacccgg cacaggtcac
      781 ctttatctgc cggtatgcat agggtggctt ccacaaaaat gccttccagc ccccgtgctc
      841 tgccagaaac gcttccagat gccgggcctc ctcccgggtc acggaaagcg tcacccggta
      901 tgttttcagg tcagcattca gccctgccgc catacgctgt gagtacccgt caccaaaacg
      961 cacttcacgc accgatggct gtgagttcac ctccatatcc ggcttcactt tccagcgaaa
     1021 ggttttcatc gcccgctccc tgataacata ccgccatcac gcaactgcag ccggagctca
     1081 tcctgcgccc ccttgcgggc catgtcatac accgccttca tcagctgcgg ccccgcctgt
     1141 ccgttggtgc cgtcgttctg aatcaccacg tgattgttct gattaaaatt aatgccttcc
     1201 gtccgccgca tctgcgccgg acttccggca ccgcccacat aaccaccttc cgcatagccg
     1261 cgcatcagac ggtaaagatt ccccacacct atccggctgg ttgcctcttt cgtgaaaaca
     1321 aactccccgc ggtgaactat ccccgcaggc tcatatttgc cgcccgtccc cgtaaatcct
     1381 ccggtcgcga aatggaagtt cgccgccgca gcctcaatgg ccgtccccga ggaagcagat
     1441 gcaccaccac cgaaagcacc gccaatggcg ctgccgatac gcccgacaat gcccaccatg
     1501 gcctgtttaa gcaggatttc tgtcatcatg gacagcac
//
LOCUS       DAZFJF010000361         1513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFJF010000361 DAZFJF010000000
VERSION     DAZFJF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.364"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..436)
                     /gene="ler"
                     /locus_tag="ACHKAU_005413"
     CDS             complement(65..436)
                     /gene="ler"
                     /locus_tag="ACHKAU_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO5534241.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
BASE COUNT          473 a          319 c          244 g          477 t
ORIGIN      
        1 gcttctttat tttttattat ttcgtcttcc agttcagtta tcgttatcat ttaatgattt
       61 catgttaaat atttttcagc ggtattattt cgtcttcggt gtccttcaca agaaaatctt
      121 ctttcttcat tccattcaac agtgcttctt taagccagcg tggctgtcgg ccaactcccg
      181 accaggtctg cccttcttca ttgcgataat aaactccttt cgatgagttc cggcgagcaa
      241 ggccatcatc aggcacatta gtatatccca gctcttgtag ggttatattg taataagtaa
      301 tctgcttttg aatcgactca attacacttt gaatttcctg ctctcgcagt cgctttgctt
      361 cctgttgtaa cacttcaatt tgctcaataa gctgaatgta tgggcttgtt gtgtgcgaat
      421 tagtttccat attcataata aataatctcc gcatgcttta atatattaag ctattagcaa
      481 tcttatcctt atcaggaagg atcaacaatt aatcgaatga tataaattat gtgagataac
      541 gttttatcta tcaaattaga acacatctac ttcatcaaac aaaatcctta taaaagttat
      601 taatcaccga tgtataatgt gtaaaacaca ttatcattaa atgtcaacaa aaaaatcata
      661 tttcttaaaa atcattcaaa atcaataaag tagaagaact tcatctcttt agcaaacgcc
      721 attaacaatt gcctttccat ttagttaatc gttttgtttt ctttaagatt agtcctatta
      781 gacaaatagc aaaatggaat aactacagca ctaagacgtt ccgctatata gctattacct
      841 gttcccctga cataaggagc agatatacct caacccccta ttccccagtg ctgaccggga
      901 gcggcggcgg tataaaaagg aacaaggaac aaatggcaga acaagcaacc atgtttgtcc
      961 cccccgcata accgtaccat tcgcattaag aaatattccc atatctatcc cgcaaaagag
     1021 gacccgtcat acatcaagtc cgttttactg ggcatcagat tatcgccgta attagctcag
     1081 tgaaagccgg gtaaacggtt agcaagctca gtggctcaaa agtgcatagc gctaagatgg
     1141 gcctggttac acctgccctt tgctgcagat cgtgttgatc ggtgtgatgc cgccacacgt
     1201 ttaagtcaat ctggaaaact cacaatgcgc tcttctataa agaggactct ttgataaagt
     1261 aacccgaaaa gggaaagcta actgatatca atgtaccacc ttagtaatca tcccttatat
     1321 acagacaaaa agaagttgcc tgaaagccag gtatctaaac taatcactaa tggtatattt
     1381 tatcagcaca ataaaaattt tttctgaatg ctattattaa tgtaaataat tacaaccttt
     1441 tcgtaggcac aaaaactatt gctgatgaat caatgaaaat ctattgttcg tcattagagg
     1501 agtatttcct cta
//
LOCUS       DAZFJF010000362         1496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFJF010000362 DAZFJF010000000
VERSION     DAZFJF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.365"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..338
                     /locus_tag="ACHKAU_005414"
     CDS             <1..338
                     /locus_tag="ACHKAU_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5534242.1"
                     /translation="PDFEREDDWWRNGQNLYLDNMEATGFYRISLPSAQPGDILLCCF
                     GASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSAWRHRHWHVSAFTGIYN
                     DLAAASACM"
     gene            335..916
                     /locus_tag="ACHKAU_005415"
     CDS             335..916
                     /locus_tag="ACHKAU_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5534243.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTIIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            1162..>1496
                     /locus_tag="ACHKAU_005416"
     CDS             1162..>1496
                     /locus_tag="ACHKAU_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534244.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRL"
BASE COUNT          349 a          378 c          474 g          295 t
ORIGIN      
        1 tgccggattt tgagcgtgag gatgactggt ggcgcaacgg tcagaacctt tacctggaca
       61 atatggaggc gactggtttt tacaggattt ccctgccttc cgcacagcct ggcgatatcc
      121 tgctgtgctg ctttggcgca tcggtggcca atcatgccgc catatactgc ggcaacggtg
      181 agctgcttca ccatctgcct gaacaactga gtaaacggga gaggtattcc gaaaaatggc
      241 aacgacgaac gcattcagcc tggcgtcacc gccactggca cgtatctgcc ttcacgggga
      301 tttacaacga tttggccgcc gcctcagcct gtatgtgaac acggcagcgg aagccatccg
      361 tgccctgtcg ctgcagatgc cgggattccg ccgtcagatg aacgaaggct ggtaccagat
      421 acgtattcgc ggtgaggaca cggcaccgga ggcggtgtac gcccgtcttc acgaacaact
      481 gggtgaggga acgatcatcc acattgtgcc gcgactggcc ggggccggaa agggtggact
      541 gcagattgtg ctgggggcgg cagccatcgt gggctctttc ttcaccgccg gcgcaacgat
      601 ggcgttatgg ggttcagccc tggcagccgg tggtttttct gccaccacga tgctgttttc
      661 actgggggcc agcatgatac tgggtggtgt ggctcagatg cttgccccga aggcaaaaac
      721 accggattac cgcgcaacgg ataacggcag acagaacacg tatttttcct cactggacaa
      781 catgattgct caggggaacc cgatgccggt gccttacggt gaaatgctgg ttggctcacg
      841 gcgaatctcc caggacatca gtacccgtga tgaaggcggt gacgggaagg tggtggttat
      901 cgggcggcag gcataaaagc gaaaaaatcc cgcagtgacc gaaggctgcg ggaacagaaa
      961 atgaagatta accacagggg gttttgtttt tattggcccg aaaaaactgt aacgcccggg
     1021 aatgatatct gccacggggg cgtacagaaa atgtgaagaa attcagaaat tttattccgt
     1081 catgacacag gcaccctccg gggtgcctgt cgtttttggg gcataaacag attcagacat
     1141 cagacaggag agggggaccg agtgggtaaa ggtggcggca gggcacacac gccggttgag
     1201 gcaaaggaca atcttaagtc cacgcagatg atgagcgtga ttgatgccat tggtgaaggg
     1261 ccgattgaag gtccggtgaa ggggctgcag agtatcctgg tgaacaaaac cccgctgacg
     1321 gacacggacg gtaatcctgt gatacatggt gtgacagcgg tctggcgcgc cggggagcag
     1381 gagcagacac cacctgaagg ctttgagtcc tccggggcgg aaaccgcact gggcgtggaa
     1441 gtgacgaagg caaagccggt gacgcgcacc attacgtccg cgaacattga ccgcct
//
LOCUS       DAZFJF010000363         1474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFJF010000363 DAZFJF010000000
VERSION     DAZFJF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.366"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1466
                     /locus_tag="ACHKAU_005417"
     CDS             <1..1466
                     /locus_tag="ACHKAU_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO5534245.1"
                     /translation="LLEKLNEREREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEAD
                     LKALQKESDTLTGRVDDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAASCCPECGGSL
                     SYLGEDAAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLAR
                     VLISKYAEHTPLYRQSEMYGRQGVELSRSLLSGWVDACCRLLSPLEEALQDYVLTDGK
                     LHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSTLAPAVLFAYSPDRKGIHPQTHL
                     AGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGE
                     LYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQ
                     WPALTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKL
                     NGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT          331 a          427 c          455 g          261 t
ORIGIN      
        1 ccctgctgga aaagctgaac gagcgtgaac gcgaaataga ccatctgcag gcacagctgg
       61 ataagctgcg ccggatgaac ttcggcagcc gctccgaaaa agtctcccgt cgtatcgcac
      121 agatggaagc tgacctgaag gcacttcaga aagaaagtga tacccttacc ggtcgggttg
      181 acgacccggc cgtgcagcgc ccgctgcgtc aaacccgcac ccgcaaaccg ttccccgaat
      241 cactcccccg cgatgaaaaa cggctgctgc cggcagcatc atgctgcccg gaatgtggag
      301 gctcactgag ctatctgggt gaggatgccg ccgaacagct ggagttgatg cgcagcgcct
      361 tccgggttat ccggactgta cgtgaaaagc atgcctgtac tcagtgcgat gccatcgtgc
      421 aggcccccgc gccttcacgg cccatcgagc ggggtatcgc aggaccgggg ctgctggccc
      481 gcgtgctgat ctcaaagtat gcagagcaca ccccgctgta ccgccagtct gaaatgtacg
      541 gccgccaggg cgtggagctg agtcgttcac tgctgtcggg ctgggtggat gcatgctgcc
      601 ggctactgtc accgctggaa gaagcgcttc aggactatgt gctgactgac ggtaagctcc
      661 atgctgatga cacgcctgtc ccggtgctgt tgccaggcaa taagaaaacg aagaccgggc
      721 ggttatggac ctacgttcgt gacgaccgta acgccgggtc aacgctggcg ccggcggtgt
      781 tgttcgctta cagcccggac agaaaaggca tccatccgca gacccatctt gcggggttca
      841 gtggtgtact gcaggcggat gcatacgccg ggttcaacga gctgtaccgg gatggccgga
      901 taacggaagc cgcctgttgg gctcacgccc gccgtaaaat ccacgatgtg cacgttcgca
      961 ccccgtcagc cctgacggag gaagcgctga aacggatcgg cgaactgtac gccatcgagg
     1021 cagagataag gggaatgacg gcggagcagc gccttgccga acgtcagttg aaaacgaaac
     1081 cgctgctgaa atccctggaa agctggctgc gtgaaaagat gaaaaccctg tcgcgacact
     1141 cagaactggc gaaagcgttc gcatacgccc tgaaccagtg gccggcgctg acgtactatg
     1201 cagatgatgg ctgggctgag gcggacaata acatcgctga aaatgcgttg cggatggtca
     1261 gtctgggccg caaaaactac ctgttcttcg gttcggatca tggaggagag cggggagcgc
     1321 tgctgtacag cctgatcggg acgtgcaaac tgaacggagt ggagccagaa agctacctcc
     1381 gctatgtcct tgacgtcata gccgactggc cgataaaccg ggtcggcgaa ctgctcccct
     1441 ggcgcgtagc actgccgact gaataacaca tccc
//
LOCUS       DAZFJF010000364         1468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFJF010000364 DAZFJF010000000
VERSION     DAZFJF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.367"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(68..472)
                     /locus_tag="ACHKAU_005418"
     CDS             complement(68..472)
                     /locus_tag="ACHKAU_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO5534246.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(693..1424)
                     /gene="bluR"
                     /locus_tag="ACHKAU_005419"
     CDS             complement(693..1424)
                     /gene="bluR"
                     /locus_tag="ACHKAU_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO5534247.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          381 a          333 c          317 g          437 t
ORIGIN      
        1 tacttttatt cccattatgc taatgcctta ctgaattatg aagcatttct taagcatcca
       61 actttagcta gattaatggt ttattatttt ctacatcttc aatatataaa agcgtattat
      121 caatggcgta gtaactgcgt ttgttatgat taacatcagt aacccaccgg aaaacgcccg
      181 cgcctgctag tgttgaacag tattcccgaa atgtagattt tccgcaaata tgaagcaatg
      241 cggcctcttt tattttagca gggttcttgg tcgtactaac ttttaacagg ttcctggttc
      301 ctcttaataa caaaaccgtg tcatcgtgag taataattct gatgttatcc gtagccagat
      361 aataaatgta atgtgcaata cggtgatgtt ttaattctga ataaaaccag gagaagtttt
      421 gctcttttct cacttgctca aacatctttt gaaaaacaac gacctgatcc atcaggataa
      481 taacctcttg ttagttgtga gactgcgtag tgtgcacgat cggttttacc acttcaatct
      541 ggtctgtcct ttggctgtga tatgtacaga gtgtgataga gggaatatct gaattctccc
      601 ggtgagcatt ttgcaacgga ccagctccgg tacaaacgct gttgtgggtt cagattataa
      661 cattctgtct aaggggcggg ataaaggtga aattaggggg catgaaagat gactttataa
      721 ccttgctcac cccaatgttg taaaagttcg ttttgccttc tcgttggtgc catgcctgtc
      781 cagacaatca atgtttgcgt cgggaacagt tccgggcgcg gcgattcaat gggatcagca
      841 agaacagaaa tgtgccatcc tgacaatgat aaacgccatg cttccagcca caatcgtgct
      901 ctctcttcaa cattccacgc catcagaatg gcttctttac cgggcttacg gcgcatttcg
      961 aaaagcgagg ttgctgcgta ttcaattaat gcgccgtcaa acatactgct cataatgcgg
     1021 gaggtgttgt gatcaagcac gagacgctgg cgaacaggaa ggtaaacatg attaatcaat
     1081 tgatctactg ggtactctcg acccagtgaa ataattctcg cgcgtagttt ggcaggatta
     1141 gccatgcgaa gaattgacat catctcttct tgcaggcggc tccagtcatc ttccgtatcc
     1201 tggctggtgg tttccagtaa tgctttaact ttgcctacag ggacgccatt acttatccaa
     1261 cgcttgatct cttcgatgcg ttgtatgtct tcttcatcaa agagtcggtg tccgccttca
     1321 ctgcgctgtg gttttaacaa accgtagcgg cgttgccagg cccggagagt gacaggatta
     1381 atcccgcaac gttcagcaac atcaccaatg ctgtaataag ccacaattcc tccttgcggt
     1441 cacaaatctc cgtcgcctgt acacgacc
//
LOCUS       DAZFJF010000365         1464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFJF010000365 DAZFJF010000000
VERSION     DAZFJF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.368"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..270)
                     /locus_tag="ACHKAU_005420"
     CDS             complement(<1..270)
                     /locus_tag="ACHKAU_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534248.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHKAU_005421"
     CDS             complement(365..571)
                     /locus_tag="ACHKAU_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5534249.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHKAU_005422"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHKAU_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          378 a          340 c          414 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tggtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agca
//
LOCUS       DAZFJF010000366         1443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFJF010000366 DAZFJF010000000
VERSION     DAZFJF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.369"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..277
                     /locus_tag="ACHKAU_005423"
     CDS             <1..277
                     /locus_tag="ACHKAU_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5534250.1"
                     /translation="AELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTG
                     ASSMGDGESPGLTSDAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            427..1044
                     /locus_tag="ACHKAU_005424"
     CDS             427..1044
                     /locus_tag="ACHKAU_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5534251.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(1232..1426)
                     /locus_tag="ACHKAU_005425"
     CDS             complement(1232..1426)
                     /locus_tag="ACHKAU_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5534252.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          381 a          320 c          369 g          373 t
ORIGIN      
        1 agcagaactt gacgccagat acacaaagga gcttgctgat gctaatgcga ctatcgaaag
       61 tctccgtgct gatgtttctg ctgggcgtaa gcgcctgcaa gtctccgcca cctgtgcaaa
      121 gtcaacgacc ggagccagca gcatgggcga tggagaaagc ccaggactta catcagatgc
      181 tgaactcaat tattaccgtc tccgaggtgg aatcgacaag ataaccgcgc aggttaacta
      241 cctgcaggaa tacatcagga cgcagtgctt aaaataattt taatttcact gaaatttaac
      301 aagtgacttt caggaaaatg cctcgcagat gcggggcatt tttgtaccgg tatttcaccg
      361 cgcaccgcag cgcacaataa acaccgaacc tgaccctttg gaatgggcct ttgaggatac
      421 cagttagtgc tggcgagcct cggtgggctg gtttcctgtg cggcaaaggt tcatttcaaa
      481 gaagcaggca acgccatgaa tgaattaatt gcgaatcatg acttcgactt tcgccagtta
      541 gttaccgcag cagaaggtca accggtaact gacaccttcc agattgccag ggcatttggt
      601 aaacgccatc agcatgtgat tagggctatt aaatgtttga gatgttctga ggaattctcg
      661 acaacccatt tttgggccgt cgagaaaatc aatgacttag gtatttttga caagaaacag
      721 atttactacc gcatggactt tagtggcttc gttatgctgg ttatgggatt taacggggca
      781 aaagccgatg ctgttaaaga agcctatatc aatgcgttta actggatgtc agcagaactc
      841 cgtaagtaca gcgaaagtta tgaagcagaa cgtaacgccg taatgctgga gtacatgaaa
      901 gagaaggatg tcgccagcat gtcaggccgt ctgctcaatc gctgggggag aacgaaaaaa
      961 cctcaattgc ttgcaaagct ggaacgtctg gagagacagg gacagttttt attaccggga
     1021 ttcgataaag gtattcaagc ctgacacatt atgcgctgta tcgtcgccgt attcccgcat
     1081 taaccatgac cgtagcccga cggggaattc cttctgcgtg agtgtgcggg aataatcaaa
     1141 aacgatgcac accgggtttt actgtgctga cagacgcagg gttaccctca tagtcgcttt
     1201 tccggtgcga tggtggaaga aaccgggatg ttcatccatc atcactttgg attgatgtat
     1261 atgctctctt ttctgacgtt agtctccgac ggcaggcttc aatgacccag gctgagaaat
     1321 tcccagaccc tttttgctca agagcgatgt taatttgttc aatcatttgg ttaggaaagc
     1381 ggatgttgcg ggttgttgtt ctgcgggttc tgttcttcgt tgacatgagg ttgccctgta
     1441 ttc
//
LOCUS       DAZFJF010000367         1433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFJF010000367 DAZFJF010000000
VERSION     DAZFJF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.370"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            221..868
                     /locus_tag="ACHKAU_005426"
     CDS             221..868
                     /locus_tag="ACHKAU_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5534253.1"
                     /translation="MPINLTPYLTASGGLGEIPQDTLSGIRILAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAGDSDVC
                     TLYDKESLKHLVNTSSPHPLSREKITESMIVKENSCYFDFTSGSIKNVRGEITRL"
     gene            complement(1128..1347)
                     /locus_tag="ACHKAU_005427"
                     /pseudo
     CDS             complement(1128..1347)
                     /locus_tag="ACHKAU_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          423 a          243 c          331 g          436 t
ORIGIN      
        1 ggatgaagta acccgacagc ttcaaaaaac cggagtctgg ctccggtttt ttgttgtcat
       61 gtcatggtga tgtttgttaa tgaagttaaa tttattttgg cttgataatg gagtcttatc
      121 ttttgcaaga ctgataatgg tgtgtgaaag cattgtgata agtaagatgc ttcaacagga
      181 aatcaagtaa aatgttaatg aagttaatgg gggttttatt atgccaataa atttaacacc
      241 atatctaaca gcgagtgggg gactggggga aataccgcag gatactcttt ctggcatacg
      301 aatactggct tttaacggag gagctcaggt tcagatgggg agcactattg ttacaatacg
      361 ttctgtcttt ttaggcttct ttatgggaag tgtaagccct gaaggattat cagagagagc
      421 tttacgaact gcattgaata atgttaatcg tcttgaacgc gaccttaatg gagggctatc
      481 cggccgccag attttagcaa gtatacccag cccgtatgct tctccacctc gtccttctgt
      541 aatgcaaaca gagttggtgt tagagcaaat agaaaaatgc tcatttaatg ttaactcgtc
      601 atctctgagg gcgactgaag aggcattaac atgcccgatt acattatgca tccctgagca
      661 tggtgtgatt atgagaaatg ctggagattc agatgtatgt actttatatg ataaagaatc
      721 attaaaacat cttgttaaca caagttcacc tcatcctctg agcagagaaa aaataacaga
      781 gtcaatgatt gttaaggaaa actcgtgcta ttttgatttc acatcaggaa gtattaaaaa
      841 tgttcgtggt gagataacta gactataaga tgttagaaca tattgactga taagtgtaat
      901 tcacagacat cttttcagtt ttttctgtta attttctaat tgatggactg tatacaggga
      961 taaaaggaga gggtattgtc tggacatttc tgttagcata attggaaatg tgcgtgaaaa
     1021 aaagcataac agacatcatg tgttatgtat gtattcagag tgcccataat tcaccagtgc
     1081 agggataaat tgtaaaaaaa tcgcagtacg tcgtatgcag aacggtgcgg cgtacgcagt
     1141 tccagtggac cgtcgcctgt gataacggtc tttgtggaat aaccgttgcg ggagttagct
     1201 cctgatctgg actgattttt ctcattccca agatggtgtg tcatctctgc attgagagcg
     1261 gcttcaacgc tgagcttttt cagcaaccga tcaaactgac tgaggtcttc tgagggggct
     1321 gaggtttttg gccagttcgt tagcaataca ccagaaaaat tctaaacgag cctgtacata
     1381 gatttgtgta attgcctgat tttgatatgt tcaacccaac atcaaatgaa ggt
//
LOCUS       DAZFJF010000368         1432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFJF010000368 DAZFJF010000000
VERSION     DAZFJF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.371"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1074
                     /locus_tag="ACHKAU_005428"
     CDS             <1..1074
                     /locus_tag="ACHKAU_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO5534254.1"
                     /translation="MRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDY
                     VVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGI
                     GVPRRVKAWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIIL
                     DTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRA
                     SLDAEYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVV
                     DDPRPPVELERIEEAGNKTENHTALWGGIRSRTQNGDKCTIPLLRDDMKKLGYEMKNF
                     RRWLYKLEKDGVIRIDGDDVAPL"
     gene            1275..>1432
                     /locus_tag="ACHKAU_005429"
     CDS             1275..>1432
                     /locus_tag="ACHKAU_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534255.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTA"
BASE COUNT          396 a          316 c          442 g          278 t
ORIGIN      
        1 atgcgggaga actggcgcga atacctggaa cgggtgcgcg ggaaagagcc ggcggaagaa
       61 aaaaaccacc agcgaaaaac cacgctaccg atgagcgttg gatctaccgg atacgacacg
      121 caactggatt acgtggttaa ggggattatt ccggcggtat cgctgtgcag catatacggg
      181 gctagcgggt cctataaatc attccttgcc ggatcgtggg cgtgccatgt tgccactggt
      241 cgccagtggg gaggccgcag ggttgcacat ggtgcggttc tctatgtggt tggtgaaggc
      301 ggtataggtg ttccgcgtcg tgtaaaagcc tgggaggttg tgcacgatga gcaggtgaaa
      361 aatctgtatc tggtaaaccg ccccatcttt ccggctgccc cgcttgatgt tgatgaaatg
      421 gttatcgctg cccgtcaggt ggagcgggaa acgggtaaac ctgtacgcat gattattctg
      481 gatacgctgg cgcgttgctt tggtgggaat gatgaaaatg attcccgtga tatgggggcg
      541 tttatccgtg gttgtgacga actgaaacga cgcacagggg ccacggtgct ggtggttcac
      601 cattccggca aggatgagac gaaaggcgcg cgcggttcca gtgcatttcg tgcttcgctg
      661 gatgctgaat accggatacg cagggaggac gcaggaagcg aagcgctggt tatctcatgc
      721 accaaaatga aggacgcgga ggaactcaaa gaagccgcat atgacttacg cgtggtggag
      781 ctttttaccg acgctgacgg tgaattaatc acgtcgctgg tggtggtgga tgatccgcgc
      841 cctcctgttg aactggagcg catcgaggag gcagggaaca agacggaaaa ccataccgcg
      901 ctatgggggg gcatccgttc acgcacacag aacggcgaca agtgcacgat cccgctgtta
      961 cgtgatgaca tgaaaaagct ggggtatgaa atgaaaaact tccggcgctg gctgtacaag
     1021 ctggaaaaag atggggttat tcgtatcgat ggggatgatg tagcgccgct ataaaagtga
     1081 ggagcaaaag cgagggggat agaaagaggg ccaaaattag cccgctctcc ctcacttttc
     1141 gacctgtata catcctcaaa agtgaggggt aaaaaaatac ttatgaaaca cacacataga
     1201 aaaaccgaaa atcccaactg cgacgaagtg agacgcttga aaaagtgagg cgaaaaagtg
     1261 agaggttgcg agaaatgacc caaaaacgca gagacagaac agagccaaaa tataaagcgt
     1321 tagacatgac tgagcacacc ttaaaggtgg caatcagaac gatagaccgc cacacgcggg
     1381 aaggatacgc gaaggaacat cccgacctga taagcgcatt catgaccacg gc
//
LOCUS       DAZFJF010000369         1428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFJF010000369 DAZFJF010000000
VERSION     DAZFJF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.372"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(161..1287)
                     /locus_tag="ACHKAU_005430"
                     /pseudo
     CDS             complement(161..1287)
                     /locus_tag="ACHKAU_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          391 a          323 c          202 g          512 t
ORIGIN      
        1 aggcttatct gtaatcggga ataatagaaa tatgttccat caatttttta agttccattt
       61 gttcacctta tttatgtaag gagtatttga tcatgtataa gcaataaaaa acagcttcag
      121 gtaatgagga atatctcaat ttttaaacat aaaatgcgaa ttatttagta caaaaagtga
      181 acttctgatg atctttccct gtgtttaacg cagccttctt tcttttcctc ttcaccctta
      241 atgtctttgc aatcccttaa taaattcagt gccatcttct ttattccaga tattatttag
      301 gctgcgtttc ctcttcttat ccggctggca tcttcattca tttttacatc taacacccaa
      361 tgaagactgt gctcgatcag ccagtgcgct ctgatagcat gtgcaaattc tttagcatcc
      421 atatcctttg atgaaatata atatcggatg cttacacctt ctgctgattt atcttctttc
      481 ttctgcctga atgacaatgc tacacaaagc ttttttaatc ccttccattc gaattcaaaa
      541 tcacaaaagt tcaggcgtta cgttactgac aatatgcaaa cgtgtttctt ttcttccatg
      601 acttatctcc tgcgtactaa acgaatcgcc tttataatta gaaaacacat ttacaggaaa
      661 tttttcctcg aatgcatgat gtaatttccc ctgattgcct tttactgcca gaagataatc
      721 tgctttttta tctttgatct tcgaagcgat atctttctga cagcccatag catcaatggt
      781 tatcaaattt ttctttaaat acagtaggtt aagcaactct ggaatggctg taatctcatt
      841 acttttggct tccgttttca cctgccccag tacaacacca ttttcgttcg agaatgcact
      901 caccatatgg attgctcctt ttctttttcc cttatcaaag gagcctctta tggtctttcc
      961 atctattgct ataatttcac catcagtgat ttcatggcac tcctgcatcc attcaataaa
     1021 catcttttca aaggccaaac tgtcaatgtt actcacaacg cgtgcaatgg tgtcatcgac
     1081 cggaatgcca ttatcaaaat caccatattt ctttagccat tcaagtcttt catgtccaaa
     1141 atcttcaatt tcctgccact catcggcacc tgcaattact gcacatacgg tgagaaacaa
     1201 aatagcagat aatttatgtt taacctttcc ttgttgtcgt atatcaggga tcactgaaat
     1261 ataatcaagc aaactttgaa tactcacctg gcgtcctttt ttatcttttt agttattcca
     1321 aaggttaaat tttgatcacc tgtaggcgtt aaagccaaga tgaaaatcgt gatttacatc
     1381 acaattttca tgatcttgcc ctgccccgtc ccttgctctc atgtcttt
//
LOCUS       DAZFJF010000370         1393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFJF010000370 DAZFJF010000000
VERSION     DAZFJF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.373"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            243..>1393
                     /locus_tag="ACHKAU_005431"
     CDS             243..>1393
                     /locus_tag="ACHKAU_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534256.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVG"
BASE COUNT          343 a          357 c          429 g          264 t
ORIGIN      
        1 ataaaagcga aaaaatcccg cagtgctcac ggacaggaac tgcgggagcg ttacgaagat
       61 tgagtgtaag gaattattct tatgtcacga caaaaaacat taacgcagag aaattattag
      121 cgccacagtc agtttgtgaa aatgtgaaga tattcagaat ttttattcag tcatgataca
      181 ggcatcctcc gggatgcctg ttgtttttgt gcgtaacagt tatcacagta aagggtgaga
      241 caatgggcaa aggtggcggc aaggcgcaca cgccggtaga ggcaaaggac aatcttaagt
      301 ccacgcagat gatgagcgtg attgatgcca ttggtgaagg gccgattgaa ggcccggtga
      361 aggggctgca gagtatcctg gtgaacaaaa ccccgctgac ggacacggac ggcaatcctg
      421 tgatacacgg tgtgaccgcg gtctggcgcg ccggggagca ggagcagaca ccaccggaag
      481 gctttgagtc ctccggagct gaaaccgcac tgggcgtgga agtgacgaag gcaaagccgg
      541 tgacgcgcac cattacgtcc gcgaacattg accgcctgcg ggtcaccttc ggggtgcagt
      601 cactggtgca gaccacctca cagggtgacc gtaacccggc atccgtccgc ctgctgattc
      661 agctgcagcg taacggtaac tgggtgacgg aaaaggatgt caccattaac ggcaagacca
      721 cctcacagtt cctcgcttcg gtgattctgg ataatctgcc tccccgcccc tttaacatcc
      781 ggatggtcag ggagacggcg gacagcacct cggaccagct gcagaacaga acgctgtggt
      841 cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcg attgtggggc
      901 tgcaggtgga tgcggagcag ttcggcggtc agcagatgac ggtgaactac catatccgcg
      961 gtcgcatcat ccaggtgccg tcaaactacg acccggaaaa acgcacttac agcggtatct
     1021 gggacggcag cctgaaaccg gcatacagca acaacccggc ctggtgcctg tgggacatgc
     1081 tgactcaccc gcgctacggc atgggaaaac gtctgggggc ggcagacgtg gacaaatggg
     1141 cgctgtatgc cattgcgcag tactgcgacc agacggtccc ggatggcttc gggggcacag
     1201 agccgcggat gacctttaat gcatacctgt cacaacagcg taaggcgtgg gacgttctca
     1261 gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa cggccagacg ctgacgttcg
     1321 ttcaggaccg cccgtcggat gtggtgtggc cgtacaccaa cagcgatgtg gtggtggatg
     1381 ataacggcgt ggg
//
LOCUS       DAZFJF010000371         1381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFJF010000371 DAZFJF010000000
VERSION     DAZFJF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.374"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1376
                     /locus_tag="ACHKAU_005432"
     CDS             <1..1376
                     /locus_tag="ACHKAU_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFO5534257.1"
                     /translation="LSMWPDNRIARDAHYLYRYDRYGRLTEKTDLIPEGGIRTDDERT
                     HRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKP
                     QVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVETATGELARTQRRSLADALQQ
                     SGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYT
                     PARKIHLYHCDHRGLPLALISKEGTTEWCAEYDEWGNQLNEENPHQLQQLIRLPGQQY
                     DEESGLYYNRHRYYDPLRGRYITQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPF
                     PLPIPWPKSPAQQQADDNAAKALTKWWNDTASQRIFDSLILNNPGLALDITMIASRGN
                     VADTGITDRVNDIINDRFWSDGKKTDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSR
                     QSNDKKR"
BASE COUNT          374 a          352 c          393 g          262 t
ORIGIN      
        1 ccctcagcat gtggccggat aaccgtatcg cccgtgacgc gcactatctt taccggtatg
       61 accgttacgg caggctgacg gagaaaaccg acctcatccc ggaagggggt atccgtacgg
      121 atgatgagcg cacccaccgg taccattacg acagtcagca ccggctggtg cactacacgc
      181 ggacacaata tgcagagccg ctggtcgaaa gtcgctatct ttacgacccg ctgggccgca
      241 gggtggcaaa acgggtatgg cgacgtgaac gggacctgac gggctggatg tcgctgtcac
      301 ggaaaccgca agtgacctgg tacggctggg acggcgaccg gctgaccacg atacagaacg
      361 acaggagccg catccagacg atttatcagc cggggagctt cacaccactc atcagagttg
      421 aaactgccac cggtgaactg gccagaacgc agcgccgcag cctggcggat gcgcttcagc
      481 agtccggcgg cgaagacggt ggcagtgtgg tgttcccgcc ggtgctggtg cagatgctcg
      541 accggctgga aagtgaaatc ctggctgacc gggtgagtga ggaaagccgc cgctggctgg
      601 catcgtgcgg cctgaccgtg gagcagatgc aaaaccagat ggacccggtg tacacgccgg
      661 cgcgaaaaat ccacctgtac cactgcgacc atcgcggcct gccgctggcc cttatcagca
      721 aggaagggac aacagaatgg tgcgcagaat acgatgaatg gggtaaccag ctgaatgaag
      781 agaacccgca ccagctgcag cagctcatcc gcctgccggg gcagcagtat gatgaggagt
      841 ccggcctgta ttataaccgc caccgctatt atgacccgct gcgggggagg tatatcactc
      901 aggatccgat tgggctgaag gggggatgga atttttatca gtatccgttg aatccagtta
      961 cgaatacaga tcctctgggg ttagaagttt ttcctagacc attccccttg ccaattccat
     1021 ggcccaaaag ccctgcacag cagcaagcag atgataatgc tgcaaaagca ttgacaaaat
     1081 ggtggaacga tacagcatca caaagaatat ttgactctct aatattgaat aatccgggac
     1141 tagcattaga tataacaatg atagcttctc gtggaaatgt tgcagacaca gggataactg
     1201 atcgtgtcaa tgacataata aatgacagat tctggagtga tgggaaaaaa accgacagat
     1261 gtgacgtact tcaggaacta attgattgtg gtgatattag tgctaaagat gcaaaaagca
     1321 cacagaaagc ctggaattgt cgtcactcca gacagtcaaa cgataaaaaa agatagccct
     1381 t
//
LOCUS       DAZFJF010000372         1371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFJF010000372 DAZFJF010000000
VERSION     DAZFJF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.375"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1371
                     /locus_tag="ACHKAU_005433"
     rRNA            <1..>1371
                     /locus_tag="ACHKAU_005433"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          347 a          310 c          424 g          290 t
ORIGIN      
        1 acgaggcact gcggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat
       61 caggtaacat caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc
      121 gcttgagaga actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc
      181 acgctgatat gtaggtgaag tccctcgcgg atggagctga aatcagtcga agataccagc
      241 tggctgcaac tgtttattaa aaacacagca ctgtgcaaac acgaaagtgg acgtatacgg
      301 tgtgacgcct gcccggtgcc ggaaggttaa ttgatggggt tagcgcaagc gaagctcttg
      361 atcgaagccc cggtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt
      421 gtcgggtaag ttccgacctg cacgaatggc gtaatgatgg ccaggctgtc tccacccgag
      481 actcagtgaa attgaactcg ctgtgaagat gcagtgtacc cgcggcaaga cggaaagacc
      541 ccgtgaacct ttactatagc ttgacactga acattgagcc ttgatgtgta ggataggtgg
      601 gaggctttga agtgtggacg ccagtctgca tggagccgac cttgaaatac caccctttaa
      661 tgtttgatgt tctaacgttg acccgtaatc cgggttgcgg acagtgtctg gtgggtagtt
      721 tgactggggc ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg
      781 tcggacatca ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc
      841 gagcaggtgc gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc
      901 aacggataaa aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg
      961 gcggtgtttg gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg
     1021 gtatggctgt tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt
     1081 tcggtcccta tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg
     1141 accggagtgg acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct
     1201 aaatgcggaa gagataagtg ctgaaagcat ctaagcacga aacttgcccc gagatgagtt
     1261 ctccctgact ccttgagagt cctgaaggaa cgttgaagac gacgacgttg ataggccggg
     1321 tgtgtaagcg cagcgatgcg ttgagctaac cggtactaat gaaccgtgag g
//
LOCUS       DAZFJF010000373         1369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFJF010000373 DAZFJF010000000
VERSION     DAZFJF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.376"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1369
                     /locus_tag="ACHKAU_005434"
     CDS             <1..>1369
                     /locus_tag="ACHKAU_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO5534258.1"
                     /translation="APTGVACSVCPGGMTSGNPVNPLLGAKVQPGETDLALPGPLPFI
                     LSRTYSSYRTKTPAPVGVFGPGWKAPSDIRLQIRDDALILNDNGGRSIHFEPLLPGEA
                     VYSRSESMWLVRGGKAAQPDGHTLARLWASLPPDIRLSPHLYLATNSAQGPWWILGWS
                     ERVPGAEDLLPAPLPPYRVLTGMADRFGRTLAYRREAAGDLAGEITGVTDGAGREFRL
                     VLTTQAQRAEEARKPHTASLSSPDSPRPLSAPSFPDTLPGTEYGADSGIRLSAVWLMH
                     DPEYPENLPAAPLVCYDWTPRGELAAVYDRSGTQMRHFTYDDKYRGRMVGHRYAGRPE
                     MRYRYDDAGRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKE
                     LADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGN
                     QLTAVV"
BASE COUNT          255 a          410 c          481 g          223 t
ORIGIN      
        1 gcgccaactg gcgtggcgtg ctcggtgtgt ccgggcggga tgacctcggg caacccggtg
       61 aacccgctgc tcggtgcaaa agtacagccc ggcgagacgg accttgcgct gcccggcccg
      121 ctgccgttca ttctctcccg cacctacagc agctaccgga cgaagacgcc tgcaccggtg
      181 ggcgttttcg gccccggctg gaaagcgcct tctgatatcc gcttacagat acgcgatgat
      241 gcactgatac tcaacgacaa cggcgggcgg agcattcact ttgagccgct gctgccgggg
      301 gaggcggtgt acagccgcag tgagtcaatg tggctggtgc gtggcggtaa ggcagcgcag
      361 ccggacggcc acacgctggc acggctgtgg gcgtcgctgc caccggatat ccggttaagc
      421 ccgcatcttt acctggcgac caacagcgca caggggccgt ggtggatact ggggtggtct
      481 gagcgggtgc cgggtgctga ggacttactg ccagcgccgc tgccgccgta ccgggtgctt
      541 accgggatgg cggaccgctt cgggcggacg ctggcgtacc ggcgtgaggc cgccggtgac
      601 ctggccgggg aaatcaccgg cgtgacggac ggtgccgggc gggagttccg tctggtactg
      661 accacacagg cacagcgggc ggaagaggcc cgtaaaccgc acaccgcttc gttatcttcc
      721 cctgacagtc cccgccctct atcagctcct tccttccccg acacactgcc cggtacggaa
      781 tacggcgctg acagcggtat ccgtctttcg gcggtgtggc tgatgcacga cccggaatac
      841 ccggaaaatt tacctgccgc gccactggtg tgctacgact ggacgccacg cggtgagctg
      901 gcggcggtgt atgaccgcag cggtacgcag atgcgccact ttacttacga tgataaatac
      961 cggggccgga tggtggggca ccgttatgca ggcaggccgg agatgcgcta ccgctacgac
     1021 gatgcggggc gggtggtgga gcaacttaac ccggcaggcc tgagttaccg ctaccagtat
     1081 gagcaggacc gcatcaccgt cacggacagc ctgaaccggc gtgaggtgct gcatacagaa
     1141 ggcggggccg ggctgaagcg ggtggtgaaa aaagaactgg cggacggcag cgtcacgcac
     1201 agcgggtatg acgcggcagg aaggctcacg gcgcagacgg acgcggcggg acggcggaca
     1261 gagtacggtc tgaatgtggt gtccggcgat atcacggaca tcaccacacc ggacgggcgg
     1321 gagacgaaat tttactataa cgacgggaac cagctgacgg cggtggtgt
//
LOCUS       DAZFJF010000374         1362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFJF010000374 DAZFJF010000000
VERSION     DAZFJF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.377"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1362
                     /locus_tag="ACHKAU_005435"
     CDS             <1..>1362
                     /locus_tag="ACHKAU_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5534259.1"
                     /translation="VYDRSGKQVRCFTYDDKYRGRMVAHRHTGRPEICYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDGLELRRE
                     YDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCS
                     GYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNIAGDLTA
                     VIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLD
                     RLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWYYDEADRLTHRTVKGETAERWQY
                     DERGWLTDISHISEGHRVAVHYGYDSKGRLASEHLTVHHPQTEALLWQHETRHAYNAQ
                     GLA"
BASE COUNT          343 a          389 c          415 g          215 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg ctgctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg accggaaatc tgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagt tacacgtatc agtatgaaaa
      181 agaccgcatc accatcaccg acagcctgaa ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcaccac gccggatggc agggcatcgg cgttttacta
      421 taaccaccac aaccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga
      481 atatgatgaa tccggccgtc tgattcagga aactgcccct gacggcgata tcacccgcta
      541 ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg
      601 gaaaaccatg acgtggagcc gttacggtca gttgctgagc ttcaccgact gttccggtta
      661 tgtaacccgc tatgaccatg accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg
      721 cctgagccag taccacgcat acgacagccg tggacagtta actgcggtga aagacacgca
      781 gggccatgaa acgcggtatg aatacaacat cgccggtgac ctgaccgccg tcattgcccc
      841 ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccatct gtaccacgca
      901 gggcgggctg acgcgcagta tggaatacga tgctgccgga cgggtcatcc gcctgaccag
      961 tgaaaacggc agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac
     1021 cggctttgac ggccgcacac agcgttatca ccacgacctg accggcaaac ttatccgcag
     1081 cgaggatgag gggctggtca cccactggta ctatgacgaa gcagaccgcc tcactcaccg
     1141 caccgtgaag ggtgaaaccg cagagcggtg gcagtatgac gaacgcggct ggctgacaga
     1201 catcagccat atcagcgaag ggcaccgggt ggcggtgcac tatggttatg acagtaaagg
     1261 ccgcctcgcc agtgaacacc tgacggtgca tcatccgcag acggaagcac tgctctggca
     1321 gcatgagacc agacatgcgt acaacgcgca gggactggcg aa
//
LOCUS       DAZFJF010000375         1361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFJF010000375 DAZFJF010000000
VERSION     DAZFJF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.378"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1361
                     /gene="rhsB"
                     /locus_tag="ACHKAU_005436"
     CDS             <1..>1361
                     /gene="rhsB"
                     /locus_tag="ACHKAU_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO5534260.1"
                     /translation="VYDRSNTQVRSFTYDDKYRGRMVAHRHTSRPEIRYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLE
                     IRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYAQLLSF
                     TDCSGYVTRYDHDRFGQMTAVHREEGLSQYHAYDSRGQLIAVKDTQGHETRYEYNIAG
                     DLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRY
                     DVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAE
                     RWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHA
                     YNA"
BASE COUNT          345 a          399 c          413 g          204 t
ORIGIN      
        1 agtgtatgac cgcagcaata cacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgagccg accggaaatc cgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagc tacacgtatc agtatgagaa
      181 agaccacatc accatcaccg acagcctgga ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg gcagggcatc
      421 ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg acgggctgga
      481 aatacgccgg gaatatgatg aattgggccg tctgattcag gaaactgccc ctgacggcga
      541 tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa cggaagatgc
      601 caccggcagc cggaaaacca tgacgtggag ccgttacgct cagttgctga gcttcaccga
      661 ctgttccggt tatgtaaccc gttatgacca tgaccgcttc gggcagatga cggcggtgca
      721 ccgcgaggaa ggcctgagcc agtaccacgc atacgacagc cgtggacagt taattgccgt
      781 gaaagacacg cagggccatg aaacgcggta tgaatacaac atcgccggtg acctgaccgc
      841 cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg gaaaggccgt
      901 ccgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg gacgggtcat
      961 ccgcctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac tcgaccggct
     1021 gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc tgaccggcaa
     1081 acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg aagcagaccg
     1141 cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggtacg acgaacgcgg
     1201 ctggctgaca gacatcagcc atatcagcga agggcaccgg gtgacggtgc attacgggta
     1261 tgatgagaaa ggccgcctga ccggtgagcg tcagacggtg catcacccgc agacggaagc
     1321 actgctctgg cagcatgaga ccagacatgc gtacaacgcg c
//
LOCUS       DAZFJF010000376         1345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFJF010000376 DAZFJF010000000
VERSION     DAZFJF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.379"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..607)
                     /locus_tag="ACHKAU_005437"
     CDS             complement(5..607)
                     /locus_tag="ACHKAU_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5534261.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEA
                     VEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            complement(582..1148)
                     /locus_tag="ACHKAU_005438"
     CDS             complement(582..1148)
                     /locus_tag="ACHKAU_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5534262.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          327 a          323 c          251 g          444 t
ORIGIN      
        1 atattcatgc aacctcactt ctgctgtttc gcagtttttt aagtttctgt tgatactccg
       61 ccttgatggc cctgcactct tcgacagtcc agcgatagcg gttatggttt gattcgattt
      121 cctctactgc ttcctgcccg atgcggctaa tcagttcgac gcgatacgga acgagatttc
      181 cgcttttgtg ctggttgcac accacgcatt gcttgtgaat attgcgttca tcaaatcgga
      241 gttgaggtgc cgcagcagtt gtccggtaat gtccggcatc ccactgagca gacgtgagcg
      301 ttccgcacga gatacatggt aagtcgcggt ctctttctct gatgaaggcg tttacggctt
      361 gttgggcttg tttaatccag taactgcggg gctttaaggc gagttttcga atcttcagtt
      421 tatctttctg tttctgctcc tctcgtcgta gtttcttctc tgctgctttt tccgcttttt
      481 cgcgttcttt acttcgtcgt tcgagtgcta tcttggttcc acaaatctca ttacaccaat
      541 attgattttg atattttggt ataaaccatt cattgcaaca tttacatttc cttcgataga
      601 ttcgcataag tgctcctttc gttgccggaa aaatcaccgt aatacttatc tcgggcttct
      661 tcagcaacta gtaccgctaa ctccagatca tcaaagcatc cgaagtgttt actcttgcca
      721 tggaatccta gcctaacatt ccattttttc tgtcgtttgt gccaagtaac ccctctgcaa
      781 cctgatttgc tattctttcg gatccttata tttcttgaat tttctattgg caggcattct
      841 cttaaatttt ctggcctatt gtcggtccta attccattaa cgtggtcaat ttgaccagca
      901 ggccaacgat tatgagttat gtaaaaaact aagacgtgag ttttatatct acgcccatct
      961 atcatgatca ttgaataacc gttggaatca aaagttccag caacactatt taatgctatc
     1021 cttccctgag tgggaacttt ccatctaaat accccggtag atttatcgaa acttagtaac
     1081 tcaaatatcc ttttaacagt taaatcttct cttttacggt tacatcgtct tcgcgctggt
     1141 ttagccatcg ccttcttcct ccgtaatggt tttctgaatt tggccacctg aacagagctc
     1201 accaaagcta tggatgtcgg tatttccaca ataccaagat ggcgaaaata actgcatgat
     1261 aagcctcagg gaaaagggaa gacactaccc ccgataattc agaaacaaat cgaaatacat
     1321 gaactaaaga aaaaaccaca acaaa
//
LOCUS       DAZFJF010000377         1342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFJF010000377 DAZFJF010000000
VERSION     DAZFJF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.380"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1342)
                     /locus_tag="ACHKAU_005439"
     CDS             complement(<1..>1342)
                     /locus_tag="ACHKAU_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO5534263.1"
                     /translation="VEKNKKDIDLLKDIISQKFGDRFQLRDINTLESFKKPQDYFFYQ
                     QEMLLRWNYAAASDQVRMNILKEYGGIYTDTDILPAYSDEVSQIINKKSDGDMFFEDL
                     KLRRFISEAILSLIKGEKYSIKHDSLDEKTRNQLNAILSEIEKLTIDNYFKPVETTVI
                     RDSFKIFKRYQKWSENNWNIRGNNNFMLTHKGSKCIDFIQSGQKKQYLELQRIRDNIS
                     YNNFFYTTNDLKSLDNVEIGGIPAKKYLEHGLFSEYRQDGTIPYVVSTLNISGPDMIM
                     RQMKKYYKSLGRIGEVHIKDNKLSDMNFMGVYASSDKENKSFNWLNPVSVGVNDITPD
                     DESSWAVRNNDINKILFEKINCHVPEKLPTSLYYEIDSRVFFHGWDNKSIQYVTEINK
                     DLIKDINLLLTSSNVDVKLLIKLDRELYAISSKIENPLALRSIRTLQLQLTNYVT"
BASE COUNT          402 a          231 c          169 g          540 t
ORIGIN      
        1 ggttacataa ttagttaatt gcagctgtaa agtccttata gagcgcaagg ctaacggatt
       61 ctctattttg ctgcttattg catataattc acgatctagc tttattaaaa gtttaacatc
      121 aacattagac gatgttaaca ataaatttat atctttaata aggtctttat taatctcagt
      181 aacatattgt atgcttttat tatcccatcc atgaaaaaaa actcgcgagt cgatttcata
      241 gtatagggag gtaggtagtt tttcgggtac atgacagttt attttttcga ataaaatttt
      301 attaatatca ttatttctaa cagcccatga gctttcatca tccggtgtaa tatcattaac
      361 accaacactc actggattca gccagttaaa acttttattt tctttatctg aacttgcata
      421 tacccccata aaattcatat cacttaattt attgtcttta atatgtactt cacctatgcg
      481 ccctagagat ttatagtatt ttttcatttg tcgcataatc atgtctgggc cagatatatt
      541 tagagtactt actacataag gaattgtacc gtcctgtcga tattcagaaa acaaaccgtg
      601 ttctaaatat ttttttgcag gtattcctcc tatttccacg ttatctaaag attttagatc
      661 atttgtggta taaaagaaat tattataact tatattatct ctgatgcgct gaagttctaa
      721 atattgtttt ttctgtcctg attgaataaa atcaatacat ttacttccct tgtgagttag
      781 catgaaatta ttattccctc taatattcca gttgttttct gaccattttt gatatctttt
      841 aaaaatttta aaactatctc taataactgt tgtctcaaca ggtttaaaat aattatctat
      901 cgtcagtttt tctatttcac tcaatatagc atttaactga ttacgtgttt tttcatctaa
      961 gctatcatgc ttaattgagt acttttcccc ttttatgagg gacagaatag cttctgatat
     1021 aaaccttctt agttttaaat cttcaaaaaa catatctcca tcactttttt tatttattat
     1081 ctgtgacact tcatctgaat atgcaggaag aatatcagta tcggtataaa tccctccata
     1141 ctctttcaat atattcattc ttacctgatc tgatgcagcg gcgtagttcc atcttaatag
     1201 catctcttgt tgataaaaaa aataatcttg tggtttttta aatgattcca atgtgttaat
     1261 atctctcagt tgaaatctat ccccgaattt ttgagatatt atgtctttta ataaatctat
     1321 atcttttttg tttttctcta ct
//
LOCUS       DAZFJF010000378         1336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFJF010000378 DAZFJF010000000
VERSION     DAZFJF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.381"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..116
                     /locus_tag="ACHKAU_005440"
     CDS             <1..116
                     /locus_tag="ACHKAU_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5534264.1"
                     /translation="PTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            127..870
                     /locus_tag="ACHKAU_005441"
     CDS             127..870
                     /locus_tag="ACHKAU_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5534265.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            867..>1336
                     /locus_tag="ACHKAU_005442"
     CDS             867..>1336
                     /locus_tag="ACHKAU_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5534266.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKEGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNP"
BASE COUNT          267 a          359 c          430 g          280 t
ORIGIN      
        1 accccaccac ggatatccgt aaggacagat gcagcaagtg catgcgcggg tgtgagatgc
       61 gcggcatggt ggctaatttt ggcggtttcc tttccatcaa taaactttcg cagtaaatcc
      121 tgttttatga cacagactga atcagcgatt ctggtgcatg cccggcggtg tgcgcctgcg
      181 gagtcgtgcg gcttcgtgat aggcaccccg gagggcgaac ggtaccagcc ctgcgtgaat
      241 atctccgcag agccggaggc gtattttcgt attgcgccgg aagactggct gcaggcagag
      301 atgcaggggg agattgtggc gctggttcac agccaccctg gtggtctgcc ctggctgagc
      361 gaggcggacc ggcggctgca gataaagagt gccctgccct ggtggctggt ctgccggggg
      421 gaaattcaca aattccgctg tgtgccgcat ctgaccgggc gtcgttttga gcacggggtg
      481 acggactgtt acaccctgtt ccgggatgca taccatctgg cagggataac gctgccggat
      541 tttgtgcgtg aggatgactg gtggcgcaac ggtcagaacc tttacctgga caatatggcg
      601 gcgactggtt tttaccgggt gcccctgtcc tctgcacagg cgggcgatat cctgctgtgc
      661 tgctttggcg catcggtggc caatcatgcc gccatatact gcggcaacgg tgaactgctt
      721 caccatctgc ctgaacaact gagtaaacgg gagaggtatt ctgaaaaatg gcaacgacga
      781 acgcattctg tctggcgtca ccgccactgg cacgcatctg ccttcacggg gatttgcaac
      841 gatttggccg ccgcctcagc ctgtacgtga acacggcagc ggaagccatc cgtgccctgt
      901 cgctgcagat gccgggattc cgccgtcaga tgaacgaagg ctggtaccag atacgtattc
      961 gcggtgagga cacggcaccg gaggcggtgt acgcccgtct tcacgaacag ctgggtgagg
     1021 gaacggtcat ccatattgtg ccgcgactgg ccggagccgg aaaggaagga ctgcagattg
     1081 tgctgggggc agcagccatc gtgggctctt tcttcaccgc cggcgcaacg atggcgttgt
     1141 ggggtgcagc cctgagtgcc ggagggctga ctgccaccac gatgctgttc tcactgggtg
     1201 ccagcatgat tctgggtggt gtggctcaga tgctggcacc gaaggcaaaa acaccggatt
     1261 accgcgcaac ggataacggc agacagaaca cgtatttttc gtcactggat aacatgattg
     1321 cccaggggaa cccgat
//
LOCUS       DAZFJF010000379         1332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFJF010000379 DAZFJF010000000
VERSION     DAZFJF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.382"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(943..>1332)
                     /locus_tag="ACHKAU_005443"
     CDS             complement(943..>1332)
                     /locus_tag="ACHKAU_005443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534267.1"
                     /translation="SLMARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYLLNH
                     YTESLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQNPFN
                     IIIVICTKEHIERLELMYQGKSDGFFT"
BASE COUNT          390 a          262 c          223 g          457 t
ORIGIN      
        1 tcacagtgac ctatatagta taaagaatat tatttatatt atttaattat agtattggtg
       61 gaactcattt cctaggtagg taggaactca tttcctaagt aggtaggaaa ccacttccta
      121 ggttacttcc taaatagatc ggaaatcatt tcctaggtgt tatttattct tatcgggtat
      181 cagttttgat aggggtatca gacttgatag gggtaacaga attctgtggg ggtagttcac
      241 tatgacctac ccctagctca ctgtgagcta ccgttacatt caatcatctt ccgatgtgta
      301 atatcgttac attcaataat tagtctatgt aacatatcgt tactaccaac tttcttacaa
      361 agagcatttc agatagctga atgtaacctc tcgttacatc catttgtcac atagcactct
      421 gtgtaatgcc tttctatgtg tgtgccataa tcccccctat attccctgtg taaatcatag
      481 aatatcccta acttattcct gtgtaattcc atactaaaaa ttcaatgaaa aaagtagctt
      541 aaaagttcaa ttcttacttt cttcactttt acatcacttt cgcaaatcct tgtgtaatgc
      601 ggcttacaga gcaccatcag aaattgttgg ctaaaaattg ttctgcttgt ttcttaatga
      661 gttttcttgt ttgtgcaaat tttaaaattt attttgtttt tacagcctta tttcgtgtaa
      721 tgcgtggcat agcaagccct tttcttgtgt tgtcaagaca tttggcaacg ttcaaacatc
      781 tgtttagaaa tgccaaattg ttgatctaac aaataaacct gtataaacag aacaccattt
      841 aatctattcc cctgtgtaat ccactacatc acacgataaa aacaaaaaac ttttaattat
      901 atttcatagc acacaaaagt caacagaagc cctctcgtgc tcctacgtaa aaaatccatc
      961 acttttcccc tgatacatca actcaaggcg ttctatgtgt tccttagtgc atattacgat
     1021 tattatgtta aatggatttt ggcctatatt ttgacctgca cccgcataaa cactggtttc
     1081 atcgcttaca gcccttgtga gacgtggtgc tgtttctagc atctgatccg acataaaacc
     1141 gatcattctc gtgtttagtg aaagtttagt gagagacatt acagaaaggc tttctgtgta
     1201 atgatttaat aggtaaaaac gatgttgatt acgcaagaaa attccttgct gatatttggg
     1261 gattctggat tttccagatc ggggggcgct gaaaatcgcg ttaagcactt ctcgcaatct
     1321 agccataagg ga
//
LOCUS       DAZFJF010000380         1324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFJF010000380 DAZFJF010000000
VERSION     DAZFJF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.383"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..410)
                     /locus_tag="ACHKAU_005444"
     CDS             complement(<1..410)
                     /locus_tag="ACHKAU_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5534268.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWR"
     gene            complement(410..739)
                     /locus_tag="ACHKAU_005445"
     CDS             complement(410..739)
                     /locus_tag="ACHKAU_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534269.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(736..>1324)
                     /locus_tag="ACHKAU_005446"
     CDS             complement(736..>1324)
                     /locus_tag="ACHKAU_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5534270.1"
                     /translation="FDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVG
                     SIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTK
                     EATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQASGTFEQNNHVVINNDGTNGQI
                     GPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          279 a          448 c          312 g          285 t
ORIGIN      
        1 atgcgccagc ggctgatcac ctcctgctcc ggatcggcgt aactgtttcc gttgacgaag
       61 ttcaccgcat ccagaaaacg ggcgtaaacc ttacgcctga ccaccgttcc gccgaccaga
      121 ctctgcatat cttccaccat accggtgacc atgccgtgca ggttagaaac cgtcagtgtc
      181 ggacgggcag cactgccctt gccgttcagt tcaaatcccg tcccctgaat gggatacgcc
      241 tgatactgtc gcccctgcca ggtgaccggc tcaccttttt cgttctgctc attacagaaa
      301 aaataacgtt ctccaccgac ctctgtcagg tcgatttccc agagcaccac gctggccgac
      361 tgctccgcac gggtgcattc attcagtgtt tcctgccgga tatcctgcat cagttcacca
      421 cctgttcaaa ctctgcgctg aactcaacac gcagcatact gacccgcgac gaccattttg
      481 cgcaggtcac ctttatctgc cgccactcat aaggcggcgt ccacagaaag gatttccagc
      541 ccccgtgctc ttccagaaac gactccagta ccgtggcctc ctcacggggg acagaaagcg
      601 tcacgctgta cgttttcagg ttggcattca gcccggcagg cgctcgctgg gaatagccat
      661 caccaaagcg cacctttctt acagaagggg ccgaagccac atccataccg ggtttcactt
      721 tccagcggaa ggttttcatc gtccacctcc ggagaacagg ccaccatcac gcatctgtgt
      781 ctgaatttca tcacgggcac ccttgcgggc catgtcatac accgccttca gagcagccgg
      841 acctatctgc ccgttcgtgc cgtcgttgtt aatcaccaca tggttattct gctcaaacgt
      901 cccggacgcc tgcgaccggc tgtcagccag actgcccggt gtaccgacat aaccaccggt
      961 ggcatagccg cgcatcagcc ggtagagatt ccccacgcca atccggctgg ttgcctcctt
     1021 cgtgaagaca aattcaccac ggtgaacaat ccccgctggc tcatatttgc cgccggttcc
     1081 cgtaaatcct ccggttgcaa aatggaattt cgccgcagcg gcctgaatgg ctgtaccgcc
     1141 tgacgcggat gcgccgccac caacagcccc gccaatggcg ctgccgatac tcccgacaat
     1201 ccccaccatt gcctgcttaa gcagaatttc tgtcatcatg gacagcacgg aacgggtgaa
     1261 gctgcgccag ttctgctcac tgccggtcag catcgcggcc atattctgtg caataccatc
     1321 aaag
//
LOCUS       DAZFJF010000381         1322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFJF010000381 DAZFJF010000000
VERSION     DAZFJF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.384"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            113..>1322
                     /locus_tag="ACHKAU_005447"
     rRNA            113..>1322
                     /locus_tag="ACHKAU_005447"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          378 a          285 c          399 g          260 t
ORIGIN      
        1 aaaaatctgg atcaagctga aaattgaaac actgaacaac gaaagttgtt cgtgagtctc
       61 tcaaattttc gcaacacgat gatgaatcgc aagaaacatc ttcgggttgt gaggttaagc
      121 gactaagcgt acacggtgga tgccctggca gtcagaggcg atgaaggacg tgctaatctg
      181 cgataagcgt cggtaaggtg atatgaaccg ttataaccgg cgatttccga atggggaaac
      241 ccagtgtgtt tcgacacact atcattaact gaatccatag gttaatgagg cgaaccgggg
      301 gaactgaaac atctaagtac cccgaggaaa agaaatcaac cgagattccc ccagtagcgg
      361 cgagcgaacg gggagcagcc cagagcctga atcagtatgt gtgttagtgg aagcgtctgg
      421 aaaggcgcgc gatacagggt gacagccccg tacacaaaaa tgcacatgct gtgagctcga
      481 tgagtagggc gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta
      541 aatactcctg actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg
      601 gcgaggggag tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcacgcttag
      661 gcgtgtgact gcgtaccttt tgtataatgg gtcagcgact tatattctgt agcaaggtta
      721 accgaatagg ggagccgaag ggaaaccgag tcttaactgg gcgttaagtt gcagggtata
      781 gacccgaaac ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag
      841 gaccgaaccg actaatgttg aaaaattagc ggatgacttg tggctggggg tgaaaggcca
      901 atcaaaccgg gagatagctg gttctccccg aaagctattt aggtagcgcc tcgtgaattc
      961 atctccgggg gtagagcact gtttcggcaa gggggtcatc ccgacttacc aacccgatgc
     1021 aaactgcgaa taccggagaa tgttatcacg ggagacacac ggcgggtgct aacgtccgtc
     1081 gtgaagaggg aaacaaccca gaccgccagc taaggtccca aagtcatggt taagtgggaa
     1141 acgatgtggg aaggcccaga cagccaggat gttggcttag aagcagccat catttaaaga
     1201 aagcgtaata gctcactggt cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat
     1261 gcaccgaagc tgcggcagcg acgctgatgc gttgttgggt aggggagcgt tctgtaagcc
     1321 tg
//
LOCUS       DAZFJF010000382         1319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFJF010000382 DAZFJF010000000
VERSION     DAZFJF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.385"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..1198
                     /locus_tag="ACHKAU_005448"
     CDS             53..1198
                     /locus_tag="ACHKAU_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO5534271.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          447 a          277 c          236 g          359 t
ORIGIN      
        1 tatgatatct gttgtcgaat cattcataat aaatgagaca ggggaatctc tcatgataaa
       61 cggacttaat aataattccg catctttagt tttagatgct gcaattagaa ttaattctga
      121 ttttaaaaag cagtggaatg atatgtcatg cgctgaaaaa ttactaaaag ttcttagttt
      181 tggtttatgg aatccaacgt atactcgtag tgaaaggcaa acatttcaag agttgttaac
      241 cgttttagaa cctgtatctc ccgcgcctaa tgaattgggc agaatatatg ctaattttgc
      301 agatggctca tccttaagaa tttccgtcac taacagcgaa cttgttgaag ccgagattcg
      361 cacaccagat aatgaaaaga ttcttatgct cctggagtca aacgaacaaa ataggttatt
      421 acaatcttta cccatcaatc tccacatgcc atacattcag gttcatcgtg ccttatctaa
      481 gatggacctg actgatcata agtcaatgca caatctactt agttttacgt caaaactatc
      541 agcaaccttg attcctcata atactcaaac agatccgctt tccgggccta caccattcag
      601 ctctatgttt atggatacat ttcgaggact cggcaatgca aagctttcac tcaatggtgt
      661 tgatatacct gtagatgcac aaaaattgct tcgcgatgca ctaggactta aagacacaca
      721 ttcatcactt gcccggaatg ttataaataa tggtatttct cgtcatcatg caaagcaaat
      781 agcaagggaa agcagcggca gcgataaaca gaaagctgaa gttgtggagt ttttatgcca
      841 ccccgaagcg gcaacagcca tatgctcggc tttctatcaa tctttcaatg tgccagcctt
      901 aatgttgaca catacaagga tctctcaagc cagagaatac aatgtggaaa gatcgttaga
      961 tgtacctaac gcgtgcatta acataagtat ctctcaatca ccagatggaa gcattcacgt
     1021 tgccagccat accgggattc tgataatggc gccagaagac cgccccaacg agttaggaat
     1081 gttgacgaac aggacctcct atgaagtgcc acaaggtgtg aaatgtgaaa tcgatgagat
     1141 ggtaagaacg ctacaaccaa ggtatggcgc atctgaaaca tacctgaaaa acatttaaaa
     1201 ataaaaaaat ggacaccaca cactttattt atcaaaacaa acaataacta atcacactca
     1261 caattattaa taatttaacc tcacagcaat catacaaaac aaagatattt atttcgcaa
//
LOCUS       DAZFJF010000383         1314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFJF010000383 DAZFJF010000000
VERSION     DAZFJF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.386"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..>1314
                     /gene="nleA"
                     /locus_tag="ACHKAU_005449"
     CDS             15..>1314
                     /gene="nleA"
                     /locus_tag="ACHKAU_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO5534272.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGINFKDLPGPL
                     KNALSNSNIPTTVSTAASTIASATTSTVTTASKDPIPWFGLTAQVVRNHGVELPIVKT
                     ENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLD
                     NPNTKYAVLTNAAANALGAVGGFAVSRFTGTDPMLSPHIGAMVGQAAGHAIQYNTPGL
                     KPDTILWWAGTTLGLADLNKAETGEGRFTDYPRIWWHAREGAIFPNKEDIARATGADI
                     RAMEEGIPVGQRHPNPEDVVIDIESNGSPHHNPSNYVDTFD"
BASE COUNT          409 a          288 c          284 g          333 t
ORIGIN      
        1 aataaggatt tatcatgaac attcaaccga tcgtaacatc cggaatcacc acacaaaaca
       61 atcgacatca tcacgcagaa caaacgtccc ctacacaaat accgcaatcc gaattaccta
      121 atggatgcga aacgggattt gttgttcata tcccagagga tatgcagcga catgcaccgg
      181 aatgcggtga aacaacagct ctactgagct tgataaaaga tgaaggtctg ctctctgggc
      241 tggataaata tcttgcacct catcttgaag aaggctctgc aggaaaaaaa gcattggata
      301 tgtttggttt attcaatgtc tctcagatgg cattagaaat acccagcacc gttccgggta
      361 tctctggtaa atatggtgtc cagctaaaca ttgtaaaacc agatattcat cctacatcag
      421 gtaattattt tttacagata ttccctttgc atgatgaaat aggtattaat tttaaagacc
      481 ttcctggtcc attaaaaaat gcattaagca acagcaatat accaaccact gtatcgactg
      541 ctgcatccac tattgcatca gccactactt cgacggtaac caccgcgtca aaagacccaa
      601 taccatggtt tggattaaca gctcaagtag ttcgtaatca tggtgtggaa cttcctatag
      661 tcaaaactga aaatggatgg aagcttgttg gagaaactcc tcttactcct gatggcccca
      721 aagcaaatta tactgaagag tgggtgatca gaccgggaga agcagatttt aaatatggtg
      781 catctccact acaggcaact ctagggctgg agtttggcgc acatttcaag tgggatttag
      841 ataaccctaa tactaaatat gccgttctta ccaatgctgc cgcaaatgcg cttggtgctg
      901 tagggggatt tgcagtatcc agatttactg gtacagatcc aatgttaagt cctcatatcg
      961 gtgcaatggt tgggcaagca gcggggcatg ccatacagta taataccccc ggattaaagc
     1021 cagacactat tttatggtgg gcaggtacga ctctggggct ggcagattta aacaaagccg
     1081 agacaggtga aggcagattc actgactatc ctcgtatatg gtggcacgca agagaaggtg
     1141 ccatcttccc taataaagaa gatattgccc gtgcaacagg ggctgatata cgagctatgg
     1201 aagaaggtat acccgttgga cagcgacatc caaatccaga ggatgtggtc atcgatatcg
     1261 aaagcaatgg ctcaccacat cataacccat caaattatgt tgataccttt gata
//
LOCUS       DAZFJF010000384         1309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFJF010000384 DAZFJF010000000
VERSION     DAZFJF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.387"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            299..592
                     /gene="iss"
                     /locus_tag="ACHKAU_005450"
     CDS             299..592
                     /gene="iss"
                     /locus_tag="ACHKAU_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO5534273.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(683..865)
                     /gene="rzoD"
                     /locus_tag="ACHKAU_005451"
     CDS             complement(683..865)
                     /gene="rzoD"
                     /locus_tag="ACHKAU_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO5534274.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1082..>1309)
                     /gene="rrrD"
                     /locus_tag="ACHKAU_005452"
     CDS             complement(1082..>1309)
                     /gene="rrrD"
                     /locus_tag="ACHKAU_005452"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5534275.1"
                     /translation="EVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQ
                     LRRWTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          332 a          317 c          302 g          358 t
ORIGIN      
        1 tcagcacagt aaaacccggt gtgcatcgtt tttgattatt cccgcacact cacgcagaag
       61 gaattccccg tcgggctacg gtcatggtta atgcgggaat acggcgacga tacagcgcag
      121 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
      181 cctcctccaa taggctgagc atccacctat atagttttaa ttttcatcaa tccatttaac
      241 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
      301 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
      361 gtttactgtt ggaaacaaac cgacagcagt aacaccaaag gaaaccatca cccatcactt
      421 cttcgtttcg ggaattggac aggagaaaac tgttgatgca gccaaaattt gtggcggcgc
      481 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
      541 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
      601 gttgcccatc gatatgggca gctctatctg cactgctcat taatatacgt ctgggttcct
      661 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
      721 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
      781 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
      841 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
      901 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
      961 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
     1021 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
     1081 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1141 tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg cctttgatat
     1201 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca cccacgttgt
     1261 aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat atcgacttc
//
LOCUS       DAZFJF010000385         1299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFJF010000385 DAZFJF010000000
VERSION     DAZFJF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.388"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..1299
                     /gene="nleA"
                     /locus_tag="ACHKAU_005453"
                     /pseudo
     CDS             6..1299
                     /gene="nleA"
                     /locus_tag="ACHKAU_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
BASE COUNT          412 a          275 c          274 g          338 t
ORIGIN      
        1 ttattatgaa cattcaaccg accatacaat ctggaatcac ctcacaaaac aatcaacatc
       61 atcaaacaga acaaataccc tctacacaaa taccgcaatc cgaattacct ctaggatgcc
      121 aagctggatt tgttgttaat attccagatg atatacagca acatgcaccg gaatgcggtg
      181 aaacaacagc tctactgagc ttgataaaag ataaaggtct gctctcaggg ctagacgaat
      241 atatagctcc tcaccttgaa gaaggatcca taggaaaaaa acattggata tgtttggttt
      301 attcaatgtt acccaaatgg cattagagat acctagttcc gtttcaggca tctctggtaa
      361 atatggtgtc cagctaaaca ttgtaaaacc agatattcat cctacatcag gtaattattt
      421 tttacagata ttccctctgc atgatgaaat aggttttaat tttaaagacc ttcctggccc
      481 gttaaaaaat gcattaagca acagtaatat atcaaccact gcagtgtcga ctattgcatc
      541 gactggaaca tcagccacta cttcgacggt aaccaccgag ccaaaaggcc caataccatg
      601 gtttggatta acagctcaag tggttcgtaa tcatggtgta gaacttccta tagtcaaaac
      661 tgaaaatgga tggaagcttg ttggagaaac accacttact cctgatgggc cgaaagcaaa
      721 ttacacggag gagtgggtta tcagaccggg agaagcagat tttaaatatg gtgcatctcc
      781 attacaggca actctagggc tggagtttgg cgcacatttc aagtgggatt tagataaccc
      841 taatactaaa tatgccgttc ttaccaatgc tgccgcaaat gcgcttggtg ctttaggggg
      901 atttgcagta tccagatttg ctagtacaga tccaatgtta agtcctcata tcggtgcaat
      961 ggttgggcaa gcagcagggc atgccataca gtataatacc cctggattaa agccagacac
     1021 tattttatgg tgggctggtg cgacactggg ggctgccgat ttaaacaagg ccgagtttga
     1081 agtagctaga ttcactgact atcctcgtat atggtggcac gcaagagaag gagctatttt
     1141 ccccaataaa gcagatattg aacatgccac aggtgctgat atacgcgcaa tggaagaagg
     1201 tatccctgtt ggacagcggc atccaaatcc agaggatgtg gtaatcgata tcgaaagcaa
     1261 tggcttacca catcataatc catcaaatca tgttgatat
//
LOCUS       DAZFJF010000386         1289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFJF010000386 DAZFJF010000000
VERSION     DAZFJF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.389"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..825
                     /locus_tag="ACHKAU_005454"
     CDS             <1..825
                     /locus_tag="ACHKAU_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5534276.1"
                     /translation="VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAE
                     YDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKG
                     GWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPY
                     PIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYL
                     PQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            822..>1289
                     /gene="ybfC"
                     /locus_tag="ACHKAU_005455"
     CDS             822..>1289
                     /gene="ybfC"
                     /locus_tag="ACHKAU_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO5534277.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEK"
BASE COUNT          405 a          264 c          310 g          310 t
ORIGIN      
        1 gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggcgcgaaa aatccacctg
       61 taccactgcg accatcgcgg cctgccgctg gcgcttgtca gcacggaagg ggcaacagaa
      121 tggtgcgcag aatacgatga atggggcaac ctgctgaatg aagagaaccc gcatcagctg
      181 cagcagctta tccgcctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      241 cgccaccgct attatgaccc gctgcagggg aggtatatca ctcaggatcc gattgggctg
      301 aaggggggat ggaattttta tcagtatccg ctgaatccgg ttcagtatat agattcaatg
      361 ggactggcat caaaatatgg acacttaaat aatggcggat atggagcgag acccaacaaa
      421 ccgcctacgc ccgatccaag taaattgccg gacatagcga aacaattaag actgccatat
      481 cctattgacc aggccagtag tgcgcctaat gttttcaaaa cattcttcag agcattaagc
      541 ccttacgact acacactgta ttgcaggaag tgggtaaaac caaatctgac ttgtacgcca
      601 caggatgatt cccagtatcc agggatggat acaaagacag caagtgatta cctgccacag
      661 acaaattggc caacaactca attaccacca ggatatactt gtgcagaacc ctatttattc
      721 ccagacatta ataaacccga tgggccagca acagcaggga tagatgattt gggtgaaatt
      781 ttagctaaga tgaaacagag aacatcgaga ggaataagaa aatgaaaaga gttttgttct
      841 ttttgctgat gatatttgtt agttttggtg ttatagctga ttgcgaaata caagctaaag
      901 atcatgattg ttttactatt ttcgctaagg gtactatttt ttccgcgttt cctgtcttaa
      961 ataataaagc catgtggaga tggtatcaga atgaggacat cggtgagtat tattggcaaa
     1021 cagaactggg tacatgtaaa aataataaat tcacaccaag cggggcgaga ctcttaattc
     1081 gcgtcgggtc actacgcctt aatgaaaatc atgccatcaa aggaacgttg caggagttga
     1141 taaatacagc agaaaaaacg gcgtttctcg gcgatcggtt tagaagttat ataagagcgg
     1201 gtatctatca aaaaaagagc agtgatccag ttcagttgtt ggcagtcctt gataattcaa
     1261 tcatggtaaa atattttaaa gatgagaaa
//
LOCUS       DAZFJF010000387         1288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFJF010000387 DAZFJF010000000
VERSION     DAZFJF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.390"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..592
                     /gene="espW"
                     /locus_tag="ACHKAU_005456"
     CDS             <1..592
                     /gene="espW"
                     /locus_tag="ACHKAU_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO5534278.1"
                     /translation="QKQIADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRM
                     FENISPDDTDKITGRKFSELGEYLYSGNVIKLSQLSIRYLPNINSISLIETKQSLLLH
                     RLYSDEVLQRNGTLIPTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAA
                     TFLRKSGAEEKDILACSPRNYGLHHNIKTWDPLVRN"
     gene            complement(720..>1288)
                     /locus_tag="ACHKAU_005457"
     CDS             complement(720..>1288)
                     /locus_tag="ACHKAU_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5534279.1"
                     /translation="DAARLESGIRISIGSGQYSVHYVQLLDGFSVEPVRGGLLDRLLG
                     REHRMERRAVALERQLNGGVDFLSSVNNYFQSVMAEHRENKTSNKILMEKINSCVFRP
                     DSNHFSCPESFLTCPITLDTPENGVFMRNSRGAEICSLYDKDALVQLVETGGAHPLSR
                     EPITESMIMRKDECHFDSKKEAFVASDA"
BASE COUNT          456 a          267 c          201 g          364 t
ORIGIN      
        1 acaaaaacaa attgcagatg aagtaaaatc acttacatat ttgattcctt cagaaaaaaa
       61 agagatgtgg atatataaaa acaatggaaa agataatgca aaaccaaact taggagagcg
      121 agacgtaaga atgttcgaaa atattagtcc tgatgataca gataagataa caggaaggaa
      181 attttcagag ttaggtgagt atctttattc aggaaatgta ataaaactca gtcagttatc
      241 aattcgttac ttacccaata tcaactcaat ctcattaata gagacaaaac agagtttgtt
      301 gctacatcga ttatattcag atgaagtact tcagagaaat ggaacactta tcccgacacc
      361 actacatgaa gaaaaatcaa ttccagctga caatataaaa acaatgctca acaacatacc
      421 gacttacaaa atgttaccgc cattcacaga aacacaaggt aattgttctt ctggcgcagc
      481 cacgttttta cgcaaatcag gcgccgaaga aaaagatatt cttgcatgta gcccccgaaa
      541 ttatgggctg catcataaca taaaaacatg ggaccccttg gttagaaatt aaggatccaa
      601 atattatata tttcatataa agcaaggaaa atattgcctg aataattgtt caggcaatat
      661 atccttacat ggcactatta taataaacta ttaatataaa aacacaccaa cagaaaaaat
      721 taagcatcac ttgcaacaaa ggcttctttt tttgaatcaa agtgacattc gtcttttctc
      781 atgatcattg attctgttat aggttctcga ctcagaggat gagctccacc agtttcaaca
      841 agttgcacta acgcgtcctt atcatataga gagcatatct cagcacctcg tgagtttctc
      901 atgaacactc cattctcagg tgtgtccagc gttatcgggc atgtcaaaaa tgactccggg
      961 caagaaaagt gattagaatc cggtctaaat acacaagaat ttattttttc cattaatatt
     1021 ttattacttg ttttattttc tctgtgttct gccatgacac tctgaaaata gttattaaca
     1081 ctacttaaaa aatcgacacc tccatttaat tgcctttcca gagccacagc ccttctctcc
     1141 attcgatgct cacgccccaa tagcctatcc agtaagcctc ctctcaccgg ttcaactgaa
     1201 aatccatcca gtagctggac atagtgaaca gaatattggc cactaccaat ggatattctt
     1261 attccacttt caagtctggc agcatcct
//
LOCUS       DAZFJF010000388         1272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFJF010000388 DAZFJF010000000
VERSION     DAZFJF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.391"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..535)
                     /locus_tag="ACHKAU_005458"
                     /pseudo
     CDS             complement(1..535)
                     /locus_tag="ACHKAU_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            512..753
                     /locus_tag="ACHKAU_005459"
                     /pseudo
     CDS             512..753
                     /locus_tag="ACHKAU_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(834..992)
                     /locus_tag="ACHKAU_005460"
     CDS             complement(834..992)
                     /locus_tag="ACHKAU_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5534280.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1078..>1272)
                     /locus_tag="ACHKAU_005461"
     CDS             complement(1078..>1272)
                     /locus_tag="ACHKAU_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534281.1"
                     /translation="GYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLSTSFNAGSKRF
                     VIYQSAQDKNLQVDIVRTRI"
BASE COUNT          375 a          330 c          261 g          306 t
ORIGIN      
        1 cgacagcatg gtaccagcag gatccccgcg ttattcggga tatacgggag cagttttttg
       61 gcaatatgta aaccctggcc gacacagccg tactgccctt tgctcaggtc agccctcgga
      121 tgattcagcg tactcatatc ctgcacatca tgcagacagt ggtcagccgg aataatatcg
      181 ttatatctgc aggcagcccc gcccggcgtc actgtactgc ggcgcgccag ctgtttaatg
      241 cgcggatccg gagcatcgta tgaatccggc agcggaagcc cttcaccgta agcccttcac
      301 cgtaagccat ggcattggat tgcccggcca gtacgatgac gtagtaccaa tccggctcag
      361 atgaagggcc gacctgtggc tctccttcaa tagccaccgc ctgcatcagt gtgtacggcg
      421 taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt gtcagctttt
      481 ccgcaaggtc tgacggcgac gccgccctga caacatcgta atgtttaatc gacatcgaat
      541 ttctcccgtg tagaggaaca gagttaaaaa gccggaagcg gaatcaaatc acaggatgac
      601 catctgccag tggcaggtca taaaaaaaag gctgcgcaat gcgcagccag aactcacaag
      661 gaaaatgata aaaggaataa cactagtgat gtacgcatgg cgcctcccgc taagttctgc
      721 aatgatcaaa cagaactcgc tacgtgccct taaaactcga tcatttagcc cctccaagga
      781 ggattcacca tgcggttgat tttttaataa acagtaaaca aaaaagtcaa gaattattca
      841 ttctgttctt tcatcatcgg ccacagcaat accacaatgc cgcagaccag agcgccatca
      901 gtcagtacca acattatcct gctggtgaaa tccatcatca ccatcactaa aagcaggatc
      961 acaacagcaa gcagacacag tttataaaac aatgttcaga aaacgcattc agcatgccta
     1021 aggttctatt cctacgaata gccaacttgc aacttaaaat attatttatg cagccaatta
     1081 aattctggtc cttacaatat caacctgaag attcttatct tgtgctgatt gataaatgac
     1141 aaacctttta ctacctgcat tgaaagaagt agacaaaacc agacaattat cataacgagc
     1201 aagaacataa taccaaccat cattataatt aatcatttca tattctttct taaactgtgg
     1261 tttgtaatat cc
//
LOCUS       DAZFJF010000389         1269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFJF010000389 DAZFJF010000000
VERSION     DAZFJF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.392"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..226
                     /gene="ydfK"
                     /locus_tag="ACHKAU_005462"
     CDS             <1..226
                     /gene="ydfK"
                     /locus_tag="ACHKAU_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO5534282.1"
                     /translation="KDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYIEGN
                     IKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            543..1133
                     /locus_tag="ACHKAU_005463"
     CDS             543..1133
                     /locus_tag="ACHKAU_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5534283.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          425 a          260 c          248 g          336 t
ORIGIN      
        1 aaaagacacc ctaaagtggt tccctgcgca gcttcctgaa gtaagaatta tcctagggga
       61 tgctgtagtg gaagtagcaa aacagggaag acctatcaat accagaacat tgcttgatta
      121 cattgaagga aacataaaga aaaaatcatg gctggataac aaagaattat tacaaacagc
      181 gatatcagtt cttaaagaca accaaaattt aaatggtaaa atgtaatata ataaacttac
      241 ttttttatca tttttccact ttaacaacat tttgctccac ttttccacga ccaaacaact
      301 tgaaatctgg ttaaaataac acgcaacact attcttcttc cttgagtccg cccggaactc
      361 gaaaaacaaa ccgagttaaa gccatttttc acaaaatcga ttttgggtct caccaaaatt
      421 acggggttgc atacgcattc gtttattttc gaacgtgtac atacaaatat gcacaaaaat
      481 aatcaaaatt attttctgag atgcattatg atatgaacac caatttcgta tagagtctca
      541 ctatgtctcg aatttttgct tactgtcgga tatcaacgct ggatcagacc atcgaaaatc
      601 aacgccggga aatcgaaagt gcaggtttta aaatcaaacc tcagcaaata atcgaagaac
      661 acattagcgg ctcagcagca accagtgagc gtcctggttt taaccggttg cttgctcgcc
      721 tgaaatgtgg tgatcaattg attgtgacaa aactggatcg ccttggttgt aatgcaatgg
      781 atatcaggaa aacagtggaa caactgaccg aaacaggtat cagagtgcat tgcttagcat
      841 tggggggcat tgacctgacc agtccaacag gaaaaatgat gatgcacgta atttcagcag
      901 tcgctgaatt tgaacgagac cttttacttg aacgcactca ttccgggata gtaagagccc
      961 gcggcgcagg gaaacgtttt ggtcgaccac ctgtgttaaa tgaagaacag aaacaggtgg
     1021 tattcgaacg aattaagtca ggtgtaagta taagtgccat tgcccgggaa ttcaaaacct
     1081 cgcggcaaac cattttaaga gccaaagcaa aacttcagac acctgacata taaaaaataa
     1141 tctcggtgtg agatgcttta cgtcttccaa gcccccttcc ttgccgtaaa tggaaagata
     1201 catctaatta tagaatttat atgttttacc ctacggcagt gctggccatt caatatcctg
     1261 tgcagttga
//
LOCUS       DAZFJF010000390         1266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFJF010000390 DAZFJF010000000
VERSION     DAZFJF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.393"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..101)
                     /locus_tag="ACHKAU_005464"
     CDS             complement(<1..101)
                     /locus_tag="ACHKAU_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534284.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSRTFKSDWFQ"
     gene            complement(269..424)
                     /gene="hokD"
                     /locus_tag="ACHKAU_005465"
     CDS             complement(269..424)
                     /gene="hokD"
                     /locus_tag="ACHKAU_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5534285.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(671..775)
                     /locus_tag="ACHKAU_005466"
     CDS             complement(671..775)
                     /locus_tag="ACHKAU_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534286.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            complement(891..>1266)
                     /locus_tag="ACHKAU_005467"
     CDS             complement(891..>1266)
                     /locus_tag="ACHKAU_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5534287.1"
                     /translation="RSHISKALSGEKMKKKDRDADLRWIHGVIVQAAWFVKASLEQNA
                     LSGNYPVTPDSWISCSERMPDTKTAVLVARDFGRKGDWRMKWATYIPGHPDANDGWII
                     PGASWIPSHWMPLPEPPQEVRQ"
BASE COUNT          297 a          350 c          289 g          330 t
ORIGIN      
        1 tgctggaacc agtcagattt gaaagtgcgg gaacgccgcc cgtgcctgct ggcaggggcg
       61 gcagagttat ccgaattgta aaatttggta tcgtgcgcca tctgttttct ctgctggcgc
      121 agcaggtgcc agttgttcag gctgacggat ggattgtaaa ccagaacgac cagaaaaaac
      181 aaaacccgcc gaagcgggtt aagtgcgggt gcgttgagga tgcctgacac atcagcggtg
      241 gcgagggatt tctcccccgc cgggtctctt actcctcagg ttcgtaagct gtgaagacag
      301 cgacctccgt ctggccggtt cggattcgta cctcgcagag gtctttcctc gttaccagtg
      361 ccgtcactat gacggttaaa cagatgacga tcagggcgat taacatcgcc ttttgctgct
      421 tcatagcctg cttctccttg cctttcggca cgtaagaggc taacctacat ttgtgagaca
      481 tagattgggc ctcagattaa tgttaagcgt cttgcaggac gcgtaatgtt aactggggct
      541 tttctctatc tgccttttgg tgttcatgcc tgagacagat agcctcaagc acccgcagca
      601 attctactta actctccttt tcccgcaaac cgtttttacc cgatatggga attcccatat
      661 cgtaatgaat tcagttccct agtcgatcca tcaaaaacac aaccaggcag taaacgccca
      721 caacagcaac aacagccagc gcaccttcca ttgccagtga tatatcatcc gacatattcc
      781 ctcctttggt gttaatcccg gcgaacgttt ttaccctcac cgacaaataa catatactaa
      841 aaaagcgata gccatagcaa cgcctgtaat tgcaaatgct tcaggccagt tcattggcgc
      901 acctcctgcg gcggttctgg tagaggcatc cagtgtgatg gtatccacga cgcaccaggt
      961 attatccacc catcattagc gtcaggatgc cccgggatgt aagtcgccca tttcattcgc
     1021 cagtcacctt tcctgccaaa atccctggca acaagaacgg ctgttttggt atccggcatt
     1081 cgctcactac agcttatcca actatccgga gttaccggat agttgcccga tagtgcattc
     1141 tgctccagtg atgcttttac aaaccacgct gcctgaacta taacgccatg aatccagcgc
     1201 aaatcagcat cgcgatcttt ctttttcatc ttttcgccac ttaaggcctt gcttatgtgg
     1261 ctgcgt
//
LOCUS       DAZFJF010000391         1240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFJF010000391 DAZFJF010000000
VERSION     DAZFJF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.394"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..599)
                     /locus_tag="ACHKAU_005468"
                     /pseudo
     CDS             complement(60..599)
                     /locus_tag="ACHKAU_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
BASE COUNT          385 a          292 c          230 g          333 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 actgaacgcc atttaccaca aagctgattc ttaattgccg cagtcgtggc atcattaaaa
      121 atgaatttat acctcacagg ttcttccgca ctgtttttct gaagtacttc cagttgtctg
      181 agggtttctg actcatccgc gcctttgcgg agttctttat tcagttttga caacaatgct
      241 tttttctgag cctttaaaag ctcttccttg tttttccaga cagccagttc acttttgtat
      301 ctggaatata actgcatctc ctgctggtac aggggcttca ttaataattt gtccactgcg
      361 gtttttcgtt ctccggactc ggcaagcgtc aggaaaaaaa gagagaccgg cccccgcagt
      421 gtattcagtc tgcagacatc aatcagattc tggcagacaa gtgaaacagc gcccagggca
      481 gacgctgcca caagcccaag gggagcctgc gtaatttgct gagcttcaat aacggcatca
      541 cgaatgagtg gaggcagcga actcagggga aactcatcac aaggctgacc gaatgacata
      601 gtaacctcac tatacacaga tataaaagca ctgcaattct ggctattaat agcaaaaata
      661 gcagtcaaat caatatgagg taaaaatttt tgttgattta atggttaaat aataaccaca
      721 gtgtgttttg ctgctgaaga aagacaaaat gaatctgtgt gtcccctcct tcccttcaga
      781 gcacatccac tattcaaatt ttacacaacc acgtacgaac aaaaagcatc atcccgggta
      841 ttgcagatac cggtttcctg ccgggaagaa tcataaacag aacaccggga ataacgcctt
      901 caggaatcac cccccctcct gaagaagaat aaaataaaga agactacagt tcctgcactg
      961 aatgctgagt acaatattct atggacagga tgacattttt ataaataaca aaaaccattt
     1021 aatactgata catcaataaa atacttcttc cctgctgaca cagacaaaat gcaccatttt
     1081 ttcttcaaat acttctgtat aatcgccagc caatgatgag aatggattaa aataacactt
     1141 cttcgacaaa cacaaaaaca gccttcattt tacatcatca tccagtttca gttaaccccg
     1201 gacgagcgac agtgtttcag tacaggaatt aaaagccagc
//
LOCUS       DAZFJF010000392         1236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFJF010000392 DAZFJF010000000
VERSION     DAZFJF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.395"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(146..217)
                     /locus_tag="ACHKAU_005469"
                     /pseudo
     CDS             complement(146..217)
                     /locus_tag="ACHKAU_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(214..402)
                     /locus_tag="ACHKAU_005470"
     CDS             complement(214..402)
                     /locus_tag="ACHKAU_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO5534288.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(399..761)
                     /locus_tag="ACHKAU_005471"
     CDS             complement(399..761)
                     /locus_tag="ACHKAU_005471"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5534289.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            complement(758..1048)
                     /locus_tag="ACHKAU_005472"
     CDS             complement(758..1048)
                     /locus_tag="ACHKAU_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5534290.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(1048..>1236)
                     /locus_tag="ACHKAU_005473"
     CDS             complement(1048..>1236)
                     /locus_tag="ACHKAU_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5534291.1"
                     /translation="SRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGRGQQW
                     LTRKLLDNGMLKVTGEAA"
BASE COUNT          274 a          300 c          287 g          375 t
ORIGIN      
        1 aactgaattg aattcaatca aaataacata agtccaacaa aaacataaag tgcgaaatga
       61 atgccagctc catttatttc gcactataaa agattaaaag ttgcaataaa ataataaaat
      121 gactcagtta cgaaaaccaa taaactgtgg ccagtagtga gtcgctcatc atcgggcttt
      181 ttggcgaatg aaatttagct acgctttcga gtctcatcgt cttcccctct tgccctgttt
      241 gaccatcagg acgccgttaa ctattacatg acgctcgcct ttgctgtctc ggttgtactt
      301 gagcactgtt cctcttgcgc aggaaagcat cctcgccact tcggtctgat tgcctcgtgt
      361 ctggataaga agctctggta tcgtttgaat tgtggcgttc atgcgttctc cagttcggtg
      421 atttttattc caagccgtcc gcctggtact ttcacaccac gaattacgcg aatgtcatcg
      481 aattgctcgt cgtcttccgc aaatccggca tggataaggg agtcgagtaa acccttcagg
      541 atgttatcta ggtcgcggcg gcgggagtct ggaacgtctg cgattacttt gatgcggagt
      601 cgtgatttgg tgaaaatatc taacttgagt tggtggatga tttgctgaac gtcttttcgg
      661 tatttctggc ctttatcgct gatgtagtat tggcttcccc gtcttcgcca gtaggtattc
      721 accgacggcg ggtatggaag cacaaactga tattcgttca tgacttaatc ttcccctcct
      781 tcagcagtat cgcctgcgtc ctgatcacgc cttcgaggtg gtaaagtctg gcgtctttgt
      841 tgtcgaggtt atgggtgcgt cggtcgattt catcgtgaca cgcgctacaa gcccatgcgc
      901 cgatcaggtc gtcaggcttc attcccgttc cgcaaattcc agccatccgg taatgtgcca
      961 gaactgtagt ttcaggattg ccattgcata tgccgtaaat acgtacctgg cattctctgc
     1021 cgcgagattc tttgcgtagg ttagccatta agcagcctcc cctgttactt tcagcattcc
     1081 gttatcgagc agctttctgg tcagccactg ttgaccacgc ccggtgattt ttgtggtgaa
     1141 cgatatctgt attccgtgat ttgtgttgac cgctgtttct ttcactgtga aatagccgcg
     1201 atccatatat tcctgcattg gcacattgcg ccggga
//
LOCUS       DAZFJF010000393         1231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFJF010000393 DAZFJF010000000
VERSION     DAZFJF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.396"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            468..683
                     /locus_tag="ACHKAU_005474"
     CDS             468..683
                     /locus_tag="ACHKAU_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5534292.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            683..1180
                     /gene="rrrD"
                     /locus_tag="ACHKAU_005475"
     CDS             683..1180
                     /gene="rrrD"
                     /locus_tag="ACHKAU_005475"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5534293.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
BASE COUNT          328 a          264 c          325 g          314 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgactgac ctgaaaaaga
       61 agacgctggc taaaaggcct tatatgtttg tagagactta tttttcacag acagcaatga
      121 tgcctgtcaa tatattatca atatgcggat tgtttcagtt acagatgcct tattaaggaa
      181 aaaaacagcc agcactgact ttcggtggag aggtgctggc tcaaaaggat agatgtactt
      241 cacatgttgc ttctatatgg cagtacattt tctgacagac agtgacggat gttgtcaaga
      301 tattgtgtca tttataacct gaatcagggg aggccggaat gttatctggc atttttagca
      361 gagcctgaat gccataatca cggctcccgg cgttggccgt cagtgggtga cactggcggc
      421 ttttttgttt ttctttactt tcattttctg tcggcggtga cggagacata catcagatgg
      481 aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt ggggacggga tactggttac
      541 tgcagctgct ggacaaagtc tctccgtccc agtgggtggc aataggtgtg ctgggaagtc
      601 tgctgtttgg cctgctgacg tatctgacaa atctttattt caagattaaa gaagataagc
      661 gtaaggctgc gagaggtgaa taatgtcgcc gtcattacgc aaggctgttg cagctgctat
      721 tggtggtggg gctgttgcca tagcgtctgt gctcatcact ggtccaggtg gtaacgatgg
      781 tctggaaggt gtcagctaca taccatacaa agatatcgtt ggcgtatgga ctgtatgtca
      841 cggacacacc ggaaaagaca tcatgcccgg taaaacgtat accgaagcag aatgcaaagc
      901 cctcctgaat aaagaccttg ccacggtcgc cagacaaatt aacccgtaca tcaacgtcga
      961 tataccggaa acaacgcgcg gcgctcttta ctcgttcgtt tacaacgtgg gcgctggcaa
     1021 tttcagaaca tcgacgcttc ttcgcaaaat aaaccagggc gatatcaaag gcgcatgtga
     1081 tcagctacgg cgctggacat acgctggcgg taagcaatgg aaagggctga tgactcgccg
     1141 cgagattgag cgtgaagtct gtttgtgggg gcaacaatga gcagggtaac cgttattatc
     1201 tccgctctgg ttatctgcat tatcgcctgc c
//
LOCUS       DAZFJF010000394         1215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFJF010000394 DAZFJF010000000
VERSION     DAZFJF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.397"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(187..621)
                     /locus_tag="ACHKAU_005476"
     CDS             complement(187..621)
                     /locus_tag="ACHKAU_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5534294.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(614..808)
                     /locus_tag="ACHKAU_005477"
     CDS             complement(614..808)
                     /locus_tag="ACHKAU_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO5534295.1"
                     /translation="MTFTVKTIPDMLLEAYGNQTEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            complement(805..>1215)
                     /locus_tag="ACHKAU_005478"
     CDS             complement(805..>1215)
                     /locus_tag="ACHKAU_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5534296.1"
                     /translation="KIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCGTLTSAQ
                     WDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQEAVDEIES
                     NHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
BASE COUNT          270 a          321 c          266 g          358 t
ORIGIN      
        1 tacacaatat ttagaaaact aatagtgcca tgtgcaattt ttaagatttt gttattaatt
       61 gtggtcgcac cttcctttct gtgtactttc cgtatagctc acaggattct gggtacaaaa
      121 aaacccgcgc atcggcgggt taagcagcgt ggcaatgtaa ccactcttat catgatatgc
      181 agatttttac gatcgtaaac tattttttcg ctgataaaat acagaggttc tccctcccgg
      241 caattcacgc tcaacatacc gatccatctc aagcctcact cccagcatca tcagcatgcc
      301 ttcaacaatc ccctccgctt tgtgaaggcg tttacctata caggtgtcag agcacccatg
      361 tttccgtgcc agcgccatga acgtctcccc caacacgtaa taatcaacca gcaagtcatg
      421 cagatcgctg ttgttcctgt taaggcgagc catacacccg catataatca tcgcgtcatc
      481 gtcacaacac tgtggacgtg attttacttt ttcggggatc agtcccttaa atccggcagc
      541 aatgggcgac catgtaacat cctcatggtt atttgccgcc catgcccccc agcgctcaag
      601 aacctgccgg atatcacgca tcagtatctt taccccatcc gcgatgaacc ataaggacgc
      661 cattgacgac ggcgtgcttt ttcccttctt tatcgccaat gtattttctg actgtggcac
      721 gattgcagtt cagtattcgg gctacctcgg tctgatttcc atatgcctca aggagcatgt
      781 caggaatggt ttttactgtg aacgtcatgc ggcctcactt ctgctgtttc gcaggtcttt
      841 aagtttctgc tgatacttcg ccttgatcgc cctgcattct tcgacagtcc agcgatggcg
      901 gttatggttc gattcgattt cgtctactgc ttcctgcccg atgcggttaa tcagttcgac
      961 gcgatacgga acgagatttc cgcttttgtg ctggttgcac accacgcatt gcttgtgaat
     1021 attgcgttca tcaaatcgga gttgaggtgt cgcagcagtt gtccggtaat gtccggcatc
     1081 ccactgagca gacgtgagcg ttccgcacga gatacatggt aagtcgcggt ctctttctct
     1141 gatgaaggcg tttacggctt gttgggcttg tttaatccag taactgcggg gctttaaggc
     1201 gagttttcga atctt
//
LOCUS       DAZFJF010000395         1207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFJF010000395 DAZFJF010000000
VERSION     DAZFJF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.398"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..606
                     /locus_tag="ACHKAU_005479"
     CDS             <1..606
                     /locus_tag="ACHKAU_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534297.1"
                     /translation="RLFAVSITSSGNPPTFSLTPDGRLTAKNADISGSVKANSGTLNN
                     VTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQVMIP
                     PVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQ
                     GTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            673..>1207
                     /locus_tag="ACHKAU_005480"
     CDS             673..>1207
                     /locus_tag="ACHKAU_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5534298.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVSG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAY"
BASE COUNT          306 a          294 c          330 g          277 t
ORIGIN      
        1 cgactgtttg cggtgagcat cacgtcatcc ggcaaccccc cgacgttttc cctgacgccg
       61 gacgggcgac tgacggcgaa aaatgcggat atcagtggca gtgtgaaagc gaactcaggg
      121 acgctcaaca acgtcacgat taatgagaac tgtcagatta aggggaaact gtcagccaat
      181 cagattgaag gcgatattgt caaaacggtc agcaagtctt tcccccgcac gaacagttat
      241 gccagtggca ccatcacggt aagaatcagt gatgatcaga aatttgaccg gcaggtcatg
      301 ataccgccag tgttattccg cggtggtaag catgagaatt tcaacagtaa taaccaacag
      361 tcatactggt attcaacctg ccggttaaga gtgacccgca atggtcagga gatttttaat
      421 cagtccacga cggatgctca gggcgtattt tcctcagtta tagatatgcc tgccggacag
      481 gggacgctga cactgacatt caccgtatct tcatcaggag cgaataactg gacaccaaca
      541 accagtatca gcgatctgct ggttgtggtg atgaaaaaat ccacagcagg tatcagtatc
      601 agctgaattt tataacccat aacgggcgtc agaaatgacg ccttttttat tgcagaaaag
      661 cgagaggtaa ttatgcgtaa actttatgcc gccattttgt ccgcagccat ctgtctggcc
      721 gtatccggtg cgcctgcatg ggcgtctgaa catcagtcca cgctgagcgc gggctatctt
      781 catgcctcga cgaacgtttc cggcagcgat gatctgaacg ggattaacgt gaaataccgt
      841 tatgagttta cggacacgct gggaatggtg acgtcattca gctatgcagg agacaagaat
      901 cgccagctta cccgttacag cgatacccgc tggcatgaag attccgtgcg taaccgctgg
      961 ttcagcgtga tggcggggcc gtctgtacgc gtgaatgaat ggttcagcgc gtatgcgatg
     1021 gcgggtgtgg cttacagccg tgtgtcgact ttctccgggg attatcttcg cgtaactgac
     1081 aacaagggga aaacgcacga tgtgctgacc ggaagtgatg acggtcgcca cagcaacacg
     1141 tctctggcgt ggggagctgg cgtgcagttt aacccgaccg aatccgtggc cattgatgtc
     1201 gcttatg
//
LOCUS       DAZFJF010000396         1206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFJF010000396 DAZFJF010000000
VERSION     DAZFJF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.399"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..198
                     /locus_tag="ACHKAU_005481"
     CDS             <1..198
                     /locus_tag="ACHKAU_005481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534299.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>1206
                     /locus_tag="ACHKAU_005482"
     CDS             234..>1206
                     /locus_tag="ACHKAU_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534300.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEYALK
                     NAIEKHIPHGMKKYSEDKLRDLGNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRI
                     NVKDVRGWDTRFDINFQMEELTAEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFV
                     SPIALNAPRDLASFRICSDFFDLLVGIKNNKLKIDFESYDKPFHLADI"
BASE COUNT          388 a          194 c          246 g          378 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc tgactaatga atatgcactc aaaaatgcta
      721 ttgaaaaaca tattcctcat ggaatgaaaa aatatagtga agataaattg agagatttag
      781 gtaatgtagg atataacgaa agccgctatg cattcaattt caaagttgat aataatgagg
      841 attataagtc tcttattagt gccacgcttg gatatccgac cagaataaat gtaaaagatg
      901 ttcgcggttg ggatactcga tttgatatta attttcagat ggaagaactg acagctgaac
      961 aagcaataat cgaaataaga tctgttgaag cattctcaac aggctctata atattcgaag
     1021 atgaaaaaga agatgtctct tttaagtgtg aatttttcgt ttctcctatt gcacttaatg
     1081 ctccaaggga tctggcaagc tttagaattt gctcggactt tttcgattta ctggttggta
     1141 ttaaaaataa caaattgaaa atagattttg aatcatatga taagcctttt catttagctg
     1201 acatta
//
LOCUS       DAZFJF010000397         1204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFJF010000397 DAZFJF010000000
VERSION     DAZFJF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.400"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1204
                     /locus_tag="ACHKAU_005483"
     CDS             <1..>1204
                     /locus_tag="ACHKAU_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO5534301.1"
                     /translation="TDMMPAYSKQVIFKIMMQTNGDNRFLEDLKLRRAISDGVLRYVN
                     NQNIDEVNYNEISDADKNIIKKILTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWP
                     DGWNIRGLNGFMLSHKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLP
                     DTDKIGGILVKKYLSGSLFSKFRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLG
                     EEFINERKLSDKAYIGVYKTTGTGKYDWLNPESIGVNDVTPADESTWCIGKGRCVDDF
                     LFKDVSKLKTENLPELFLTKIDTDTFFSQWSTKTKKDLQKKIQDLTVRYNELIDSSTI
                     DFKNLYEIDQMLHMIMLEMNDDIAKRSLFSLQVQIAEKIRRMTIPVDNIINIYPDLHK
                     KNDNDLSMS"
BASE COUNT          444 a          198 c          228 g          334 t
ORIGIN      
        1 cacagacatg atgcctgcat actctaaaca agtaattttt aaaattatga tgcagacaaa
       61 cggagataat cgtttcttgg aagatttgaa actacgtcgt gcaatatctg atggtgtatt
      121 aagatatgtt aataaccaaa atattgatga agttaactat aatgaaatca gtgatgcaga
      181 taaaaacatt atcaagaaga tattaacaga aatatctaaa atgccagaag atagcatttt
      241 tactaagatc aatacaagga ttcctcgaga cacaatgccc atccttcgtc gttatcacct
      301 atggcctgat ggatggaata ttcgtgggct caatggattc atgctatcac ataaaggtag
      361 tgaagtgatt gatgctgtta tcgcaggcca gaatcaggct tacagggaac taagaagaat
      421 aagagataat attcatagtg aaatatactt caaacaaact gatgaattgt cctcacttcc
      481 agatacagac aaaattggag ggattctggt aaaaaaatac ctttcaggaa gtcttttttc
      541 aaaattcaga caagatacta ttatcccgga ggcattgagc accctccaga tatcaggtcc
      601 tgacttgatt caaagaaaga tgttgcaatt tttcaggagt agaggggtgt taggtgaaga
      661 gttcattaat gaaagaaaac tgagtgataa agcttatatt ggtgtctaca aaacaactgg
      721 cacagggaaa tatgactggt taaaccctga atcgatcggc gttaatgatg tcacgcccgc
      781 agatgaaagt acctggtgta taggaaaagg ccggtgtgtt gatgacttcc tgttcaaaga
      841 tgtttcaaaa ctaaaaacag aaaatcttcc agaattattc ttaacaaaaa tagatactga
      901 tacgtttttc tctcagtggt caaccaaaac caagaaagat ctgcaaaaaa aaatacaaga
      961 ccttactgta cgttataatg agctaattga ctcgtcgact atcgacttta aaaatctata
     1021 tgaaatagat caaatgctcc atatgattat gctagagatg aatgatgata tagccaaaag
     1081 gtcattgttt tcattgcaag ttcaaatagc cgaaaaaatt cggaggatga ccattcctgt
     1141 agacaatata attaacatct atcctgatct acataaaaaa aatgacaatg atctgagtat
     1201 gtcc
//
LOCUS       DAZFJF010000398         1199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFJF010000398 DAZFJF010000000
VERSION     DAZFJF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.401"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..634)
                     /locus_tag="ACHKAU_005484"
     CDS             complement(<1..634)
                     /locus_tag="ACHKAU_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO5534302.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISL"
     gene            815..967
                     /locus_tag="ACHKAU_005485"
                     /pseudo
     CDS             815..967
                     /locus_tag="ACHKAU_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(992..>1199)
                     /locus_tag="ACHKAU_005486"
     CDS             complement(992..>1199)
                     /locus_tag="ACHKAU_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534303.1"
                     /translation="AKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLN
                     VAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          376 a          199 c          224 g          400 t
ORIGIN      
        1 aaagagatat tttttgagct gaaatatcat gaacactgat tcctaagcca ttaaaactgt
       61 cctcgaagtc attaaattta tttagtcttg atgaaagtaa tttggtttta atgctttgca
      121 ggataagagg ttggtatcct gtcgagggag ttgaattaat gccattttcg atacattctt
      181 tgattattcg tattggacta ttataaccat atttatttat tttttcatga aaagataaat
      241 ttaattgttg gctatgaaaa ttgctgccat ttccatatct aaaatgttca atcagatctt
      301 cgataagtgt tttatattgt ccaaaaaagg aaagcatttt tgctagttct ttcatttctg
      361 caaataaaat atcagtgcat tcacgttgtg atatttttgt tccggaactt gaggcactaa
      421 aagcaacatt aatagaacct gggccaggga aatggtattt aataagtctg taaggatcaa
      481 cttttgcaga gatatcgctt aggccgagtg agagaaagtc ttgttctacc atatcaccat
      541 attgcatatc atcagcagaa aaatcatcga ataaactaat agtttcgaaa agtgtacagg
      601 gaatattaat tggcggaagt aatatgttat tcatttgcat ttgccttggt tatatttaag
      661 ctcaatatca attcgagaac aaatgctagg gtaaaaaaat atttaaaaaa gaagtaaact
      721 tcacattttt tcattacatc tctgaagaga acctcgttaa tgattggtct tgctcattgt
      781 taataacaaa atgttgaatt taccgattag gaaacttttt cctacgcgga ttgcctccag
      841 ctatggcatg aactttgagt ttatgccaga actgaagtgg ggcttcggct accctggcgc
      901 gattatcttt atgatcctcg cgggcctgac accgtatctg tactttaagc gtaagaactg
      961 gctgtaacta tacgatgtcg ctgtgagtaa gttagtgtgc tgacagcgac attttagccg
     1021 agggaatcgc agataacata attgttgtga ataatatcca tgccacgttg agttggacgc
     1081 ttaatgactc acatctcacc ccttcaattg ctgcagagat aatagaaaaa agtgtcaagc
     1141 ccaatgaaat caatacaaag tagattgcct tacatccagt atctcttatt ctttttgct
//
LOCUS       DAZFJF010000399         1195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFJF010000399 DAZFJF010000000
VERSION     DAZFJF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.402"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..228
                     /locus_tag="ACHKAU_005487"
     CDS             <1..228
                     /locus_tag="ACHKAU_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534304.1"
                     /translation="RVDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQE
                     EQERRKNIEKLIQIERNAKARIRRATKSRTE"
     gene            279..491
                     /locus_tag="ACHKAU_005488"
     CDS             279..491
                     /locus_tag="ACHKAU_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534305.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GADPNYCWREVDPVDAANEAMSYHND"
     gene            524..741
                     /locus_tag="ACHKAU_005489"
                     /pseudo
     CDS             524..741
                     /locus_tag="ACHKAU_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
     gene            743..1006
                     /locus_tag="ACHKAU_005490"
     CDS             743..1006
                     /locus_tag="ACHKAU_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534306.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            1017..1142
                     /locus_tag="ACHKAU_005491"
                     /pseudo
     CDS             1017..1142
                     /locus_tag="ACHKAU_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          397 a          209 c          295 g          294 t
ORIGIN      
        1 cgggttgacg aaaaagagcg ccaggtccgc tgtaaaatct gcggtgcggt tgtggagccg
       61 tttgactgga tgctctctgt ggcgaaaaga gaaaccagac tggcagatga tgtaaggcta
      121 ttgcgccagg aggaacagga aaggcggaaa aatatagaaa agttaattca gattgaacgt
      181 aacgcgaaag cgcggatacg cagggcgaca aaatccagaa ctgaataaat aaatttagcg
      241 ctgtaaataa aatctaatcc tgaactggag gtatatttat gttaaataca cagaaagcca
      301 ttaatgcgga aaaatataac gagtgggcaa gaaaattctc tgagcagatt tttaaaatta
      361 ctggcgatga gaatgcggca aaaaatgaat tagaaccgtg gacgcctgaa ggagccgacc
      421 caaattattg ctggagggag gttgatccag ttgatgctgc aaatgaagct atgagttatc
      481 acaacgatta atgtcaggag gccgcccgaa agggcggtaa tgaatggtca cattatttag
      541 aaaaaatatc cgcgaaagag tagaacaaca gaatttctgt ttctcattct gtttatcgtg
      601 ttgatgatac cgatatcccc gttaatccta gtctggataa tcggaaaaat aattgagcca
      661 gttattgaat tgtataacga cgtggtatgg gcgtcattca acacactgca caataaaatt
      721 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      781 cgaattacag gaagttcttg atcgctgtcc atcaccgaaa aaaattggct tttacaccat
      841 tggcgataaa agcatttacc tgtatgacct acgccgcatg gatgaaatca tggaggctct
      901 tgataatcgt tcgtcgatgg attggtgtgt tgctgttcat gatatgaatg cagggtttga
      961 tgaaaagatt ttgttcccct catcagttga aagcactgcg ggttaaggag taacacatga
     1021 ccactattac caaagaacgt attgaattgt tcattaaaaa tccgcttgaa aacgggctta
     1081 cccgtggtga acaaatggaa ctggcacgga ttgcgctggc atcgctggaa gcagagccgg
     1141 tggcaaagat tatagctcat tacccattag gggttgacgt aggcaaacaa aaatt
//
LOCUS       DAZFJF010000400         1183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFJF010000400 DAZFJF010000000
VERSION     DAZFJF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.403"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            55..144
                     /locus_tag="ACHKAU_005492"
     CDS             55..144
                     /locus_tag="ACHKAU_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534307.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            164..>1183
                     /gene="nleL"
                     /locus_tag="ACHKAU_005493"
     CDS             164..>1183
                     /gene="nleL"
                     /locus_tag="ACHKAU_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO5534308.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCY"
BASE COUNT          364 a          181 c          246 g          392 t
ORIGIN      
        1 ggggaatatt tgttagataa aaaagaggag ataattcaat agggagttaa attaatgccg
       61 ataaatctga catcttattt gtgggtacag ggacagaaag ttgtcccggc agttgtgttt
      121 tctaatttta ctttagtgga ctaagtaaaa aggagtgaga taaatgctgc ccactacaaa
      181 tatctctgta aattctggag taatatcttt tgaaagtcct gtagattcac catctaacga
      241 ggatgttgaa gttgccctcg aaaagtggtg cgctgaggga gaatttagcg aaaatcgtca
      301 tgaggttgca tcaaaaatac ttgatgttat aagtactaat ggagagactt tatcaatcag
      361 tgagccaata acaacattac cagacttgct tccaggttct ctgaaagaac tggttttgaa
      421 tggatgtaca gagcttaaat caataaactg cttacccccc aacttatctt cattaagtat
      481 ggttggatgc tcatcattag aggttataaa ttgcagcata cctgaaaatg tcattaattt
      541 atctttatgc cattgtagtt ctttgaaaca tatagaaggt tcctttcctg aggcactcag
      601 aaattccgta tatttaaatg gctgtaattc attaaatgaa tcgcaatgtc aattccttgc
      661 atatgatgtc agtcaaggcc gtgcctgcct gagcaaagct gagcttactg ctgacttaat
      721 ttggttgtcg gctaaccgaa cgggtgaaga gtctgctgaa gaattgaatt actctggatg
      781 tgacttgtca ggtctaagtc ttgtagggct gaatttatca tcagtaaatt tttctggagc
      841 agtgcttgat gatacagatc tcaggatgag tgatttgtct caggctgtat tggaaaactg
      901 ttcttttaaa aactcgattt tgaatgaatg taatttttgt tatgctaatt tatctaattg
      961 tattattagg gctttgtttg aaaactctaa tttcagcaat tccaatctta aaaatgcatc
     1021 atttaaagga tcttcatata tacaatatcc tccaattttg aacgaggctg atttaacagg
     1081 agctattata attcctggaa tggttttaag tggtgctatc ttaggtgatg taaaggagct
     1141 ctttagtgaa aaaagtaata ccattaatct aggagggtgt tac
//
LOCUS       DAZFJF010000401         1169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFJF010000401 DAZFJF010000000
VERSION     DAZFJF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.404"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            239..1063
                     /gene="gadX"
                     /locus_tag="ACHKAU_005494"
     CDS             239..1063
                     /gene="gadX"
                     /locus_tag="ACHKAU_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO5534309.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          341 a          222 c          246 g          360 t
ORIGIN      
        1 attaaatgta gcgattgcct ttaatgtcac gaaaagggat tatttgcttg ctattaattt
       61 cactgcgttc ttaaacacgt ttttgtatta taaatccagt catcctgccc gtagaacatc
      121 acacattatc atcctgttct cccgcttcgt ttaaatttat ttatcaatca atttgactta
      181 agagggcggc gtgctacatt aattaacagt aatatgttta tgtaatatta agtcaactat
      241 gcaatcatta catgggaatt gtctaattgc gtacgcaaga cataaatata ttctcaccat
      301 ggttaatggt gaatatcgct attttaatgg cggtgacctg gtttttgcgg atgcaagcca
      361 aattcgagta gataagtgtg ttgaaaattt tgtattagta tcaagggata cgctttcatt
      421 atttctgccg atgctcaagg aggaggcatt aaatcttcat gcacataaaa aaatttcttc
      481 attactcgtt catcactgta gcagagatat tcctgttttt caggaagttg cgcaactatc
      541 gcagaataag aatcttcgct atgcagaaat gctacgtaaa agagcattaa tctttgcgtt
      601 gttatctgtt tttcttgagg atgagcactt tataccgctg cttttgaacg ttttgcaacc
      661 gaacatgcga acacgagttt gtacggttat caataataat atcgcccatg agtggacact
      721 agcccgaatc gccagcgagc tgttgatgag tccaagcctg ttaaagaaaa aattgcgcga
      781 agaagagaca tcatattcac agttgcttac tgagtgtaga atgcaacgtg ctttgcaact
      841 tattgttata catggctttt caattaagcg agtcgcagtg tcctgtggat atcacagcgt
      901 gtcgtatttc atttacgtct ttcgaaatta ttatgggatg acgcccacag agtatcagga
      961 gcgatcggcg cagggattgc cgaaccgtga ctcggcggca agtattgttg cgcaaggaaa
     1021 tttttacggc actgaccgtt ctgcggaagg aataagatta tagaatttta ctcagacata
     1081 aaaaaaaccc ggcatagggg accgggaaga ggatagtctg ccgtctccag actaataaac
     1141 cgttataaca ctccctgttg gcacgggaa
//
LOCUS       DAZFJF010000402         1164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFJF010000402 DAZFJF010000000
VERSION     DAZFJF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.405"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..410)
                     /locus_tag="ACHKAU_005495"
     CDS             complement(<1..410)
                     /locus_tag="ACHKAU_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5534310.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWR"
     gene            complement(410..739)
                     /locus_tag="ACHKAU_005496"
     CDS             complement(410..739)
                     /locus_tag="ACHKAU_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534311.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRVPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            complement(736..>1164)
                     /locus_tag="ACHKAU_005497"
     CDS             complement(736..>1164)
                     /locus_tag="ACHKAU_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5534312.1"
                     /translation="VGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGE
                     FVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFEQNNHVVINNDG
                     TNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          242 a          397 c          278 g          247 t
ORIGIN      
        1 atgcgccagc ggctgatcac ctcctgctcc ggatcggcgt cactgtttcc gttgacgaag
       61 ttcaccgcat ccagaaaacg ggcgtaaacc ttacgccgga ccaccgttcc gccgaccaga
      121 ctctgcagat cttccgccat cccggtgacc ataccgtaca ggttagaaac cgtcagcgtg
      181 gggcgcgtac tggtgccttt gccattcagt tcaaaaccgc tcccctgaat gggatacggc
      241 tgatactgtc gcccctgcca ggtgaccggc tcaccttttt cgttctgctc attacagaaa
      301 aaataacgtt caccaccgac ctctgtcaga tcgatttccc agagcaccac gctggccgac
      361 tgctccgcac gggtgcattc attcagtgtt tcctgccgga tatcctgcat cagttcacca
      421 cctgtttaaa ctctgcgctg aactcaacac gcaacatact gacctgcgac gaccattttg
      481 cgcaggtcac ctttatctgc ctgtaaccat aaggcggcgt ccacagaaag gccttccagc
      541 ccccgtgctc agccagaaac gactccagcg ccgtggcctc ctcacgggag acagacagcg
      601 tcacgctgta cgttttcagg tcagcgttca gcccggcagg cacacgctgg gaatagccat
      661 caccaaagcg cacctccctg acggaagggg ccgatgtcac atccataccg ggtttcactt
      721 tccagcggaa ggttttcatc gtccacctcc ggagaacagg ccaccatcac gcatctgtgt
      781 ctgaatttca tcacgggcac ccttgcgggc catgtcatac accgccttca gagcagccgg
      841 acctatctgc ccgttcgtgc cgtcgttgtt aatcaccaca tggttattct gctcaaacgt
      901 cccggacgcc tgcgaccggc tgtctgccat gctgcccggt gtaccgacat aaccgccggt
      961 ggcatagccg cgcatcagcc ggtaaagatt ccccacgcca atccggctgg ttgcctcctt
     1021 cgtgaagaca aactcaccac ggtgaacaat ccccgctggc tcatatttgc cgccggttcc
     1081 cgtaaatcct ccggttgcaa aatggaattt cgccgcagcg gcctgaatgg ctgtaccgcc
     1141 tgacgcggat gcgccgccac caac
//
LOCUS       DAZFJF010000403         1164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFJF010000403 DAZFJF010000000
VERSION     DAZFJF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.406"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..857
                     /locus_tag="ACHKAU_005498"
     CDS             48..857
                     /locus_tag="ACHKAU_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5534313.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            850..>1164
                     /gene="yqeJ"
                     /locus_tag="ACHKAU_005499"
     CDS             850..>1164
                     /gene="yqeJ"
                     /locus_tag="ACHKAU_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO5534314.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKV"
BASE COUNT          400 a          216 c          219 g          329 t
ORIGIN      
        1 gcaaactatg tgatctccat ttcgattgat ttagtgttta ttgacgtatg tactggatta
       61 ttaacgataa tatcgagttc tggcctgagc accgaaaatt aatatcggta cataacgccg
      121 atcttaacgt cgttctgaca acgccagcca gtcgatgttt atcacttcta cttgaagctt
      181 ttcctgatgt ggttgcacaa caagattttt tcaccagagt ctgggaagaa gaaggtatgc
      241 gtgtgcctac taacacgtta tatcagaaca tatccattat cagacgcgga tttcgcgctg
      301 ttggtgatac tacccactcg ctaattgcaa ccgtgccgag aagaggattc aagatccata
      361 atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat acacacaatg
      421 ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc gcaaagaatt
      481 tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc gcatttgtca
      541 taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca tttttcaagg
      601 attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa gatacaattg
      661 acgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg attaactgta
      721 aaaaacatcc gtggttatac ttccctcttg caaaatcctc accggggatg attgttatgg
      781 catgcaataa aaattataac caacatgaag tggcagactg tttaaccctg tcttaccgtg
      841 aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt tatcagtggg
      901 tttattctct tcactgtcta ttcttacact gcagaaaaaa tgatatataa cgaaacatgc
      961 actgcaaatt gggtaatttt taatgaccag ggacgagcaa atcttaccat cgactttatg
     1021 tataacaaaa aaaataaaac aggaactgtt gcgttgagtg gaacctggca acaaggtaac
     1081 agagaaagta aatcaatacg gcgaaatatt gaatacacat ggattgaaaa ctatgacaca
     1141 gcccatttaa catcaaagaa agtt
//
LOCUS       DAZFJF010000404         1163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFJF010000404 DAZFJF010000000
VERSION     DAZFJF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.407"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..69
                     /locus_tag="ACHKAU_005500"
     CDS             <1..69
                     /locus_tag="ACHKAU_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534315.1"
                     /translation="DGPWQDVKGLDEVGSDTGRTGE"
     gene            71..340
                     /locus_tag="ACHKAU_005501"
     CDS             71..340
                     /locus_tag="ACHKAU_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5534316.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(486..1076)
                     /gene="tccP2"
                     /locus_tag="ACHKAU_005502"
                     /pseudo
     CDS             complement(486..1076)
                     /gene="tccP2"
                     /locus_tag="ACHKAU_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT          311 a          216 c          325 g          311 t
ORIGIN      
        1 gacgggccgt ggcaggatgt taaggggctt gatgaagtgg gcagtgatac aggcagaaca
       61 ggagaatgac atgaatatac taaaaaaact tatgcagcgt ctgtgtggtt gcggaaagca
      121 tgatggccgt gaacacgtgc agtcgcttac agcacagctg cgactgggac cggcagacat
      181 tctggagtcc gatgagaatg gcattatccc ggagcaggcc agggtaatca cgcaggtggt
      241 gatactggat gcggataaaa agcagataca gtgtgtggta agaccgctgc aaatcctgcg
      301 tgctgacggg acgtgggaaa atattggcgg aatgaaatag ccgacagctt cacaaaaacc
      361 ggagtccggc tccggttttt gttggttaga tgtaatctga cagatacctg tataaataac
      421 cggtaactgt caggtcagag ctaatacagg taattatatt ataatcacga gcgcttagat
      481 gtattaatac catgctctgc aagatgctgc atcagacgct gagccacatc aggcagaggt
      541 cttgattgtt cattttgtac cggcggcgtt ggcgcaggcc agttaggtgc cggaggaata
      601 tattcagcca tattccgggc tggttgaatg ccatgctctg caagatgctg catcagacgc
      661 tgagccacat caggcagagg tcttgattgt tcattttgta ctggcggtgg tggcgcaggc
      721 cagttaggtg ccggaggaat atgttcagcc atattccggg ctggttgaat gccatgctct
      781 gcaagatgct gcaccagacg ctgagccaca tcaggtagag gccgatgtaa agcgtttttt
      841 tctatacttg actgagtata agatgtggga cgttgaagtc ctgattctgt ataagaattt
      901 tttaccttaa aaatagttgt tgcactggca ggactgttgc tgggagaaaa aggtgagctg
      961 gtttttaccg tgttaggtgt gcttttgtgc tgcgagacag tgaaggtact gctgcgtata
     1021 gcacttgata tactgcgagg aaggctggaa aaaaaagaat taatgctatt tatcatatag
     1081 ataaccttat aagagcttta ttggatagtc ttatttatcc gtttacatga aatctacctt
     1141 ttgtgaaatt ctcgtcaata ttt
//
LOCUS       DAZFJF010000405         1148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFJF010000405 DAZFJF010000000
VERSION     DAZFJF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.408"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..206
                     /locus_tag="ACHKAU_005503"
     CDS             <1..206
                     /locus_tag="ACHKAU_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534317.1"
                     /translation="HAVRQAKRKANMTYMKAYQKAWKEHRDRYQQDMEKLESENMELR
                     RKLGEAKRDIDAYKRLFNGESHA"
     gene            166..375
                     /locus_tag="ACHKAU_005504"
     CDS             166..375
                     /locus_tag="ACHKAU_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO5534318.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEYCCAELMSDPNSSMYEEEDDE"
     gene            368..1090
                     /locus_tag="ACHKAU_005505"
     CDS             368..1090
                     /locus_tag="ACHKAU_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5534319.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEETAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTREAA"
BASE COUNT          361 a          240 c          293 g          254 t
ORIGIN      
        1 accacgcagt tcgtcaggca aagagaaagg ccaatatgac atacatgaag gcgtatcaaa
       61 aagcatggaa agaacaccgc gatcgatacc aacaagacat ggaaaagctt gaatcagaaa
      121 acatggaatt aagacgaaag ctcggtgaag caaaacgaga cattgatgct tacaagcgac
      181 tttttaatgg tgaaagccat gcttagccca tcccaatccc ttcaatacca gaaagaaagc
      241 gtcgagcgag ctttaacgtg cgctaactgc ggtcagaagc tgcatgtgct ggaagttcac
      301 gtgtgtgagt actgctgcgc agaactgatg agcgatccga atagctcaat gtacgaggaa
      361 gaagacgatg aatgagttaa taaatggcaa tgccatcaaa atgacaagca ttgaaatagc
      421 tgagttggtg ggtaagcgtc atgacaatgt gaaacgtacc atcgaaacgc tggctaaaaa
      481 tggtgttatc cggcttcctc aaattgaggt ttccgaaaga atcaataact tagggttcaa
      541 tgttcagtac gagcattacg tcttcgaggg cgaacaaggt aagcgagaca gtattgtcgt
      601 tgttgcccag ttgtcgccag agttcaccgc tcgtcttgtt gaccgttggc gagagcttga
      661 agaaactgcg gttaatatcc ccaaaacgtt accggaagcg ttgcgccttg ctgctgatct
      721 tgctgagcag aaaatgcaac tggaaaacca gctcgcaatt gccgcaccta aagttgagtt
      781 tgccgatcgc gttggcgagg ccagcggaat tttgattgga aactttgcaa aggttgttgg
      841 tattggtcca aacaaactgt ttgcgtggat gcgcgatcac aaaatcctta ttgcttcagg
      901 ctcccggcgc aatgtgccaa tgcaggaata tatggatcgt ggctatttca cagtgaaaga
      961 aacagcggtc aatacaaatc acggaataca gatatcgttc accacaaaaa tcaccgggcg
     1021 tggtcaacag tggctgacca gaaagctgct cgataacgga atgctgaaag taacaaggga
     1081 ggctgcttaa tggctaaacc agcgcgaagg aaatgcaaaa tatgcaagga atggtttcac
     1141 ccggcatt
//
LOCUS       DAZFJF010000406         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFJF010000406 DAZFJF010000000
VERSION     DAZFJF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.409"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..779
                     /locus_tag="ACHKAU_005506"
     CDS             <1..779
                     /locus_tag="ACHKAU_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5534320.1"
                     /translation="TRHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVRR
                     FRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEY
                     FWRHILQCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPV
                     HPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAV
                     DRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          247 a          308 c          302 g          270 t
ORIGIN      
        1 tcacccgtca cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcctcac
       61 tctgtaatgc actgtttcag ggggaggtca ccccggtcag tgatgttcac gccggcaccc
      121 gggaagtacg gcgcttccgt ctgagtggcc acggtcacag catggttatc actgacctgc
      181 ccggtgtggg cgagagccgg gacagggatg cagagtatga ggccctgtac cgtgacattc
      241 tgcctgaact tgacctggta ctgtggctga ttaaagccga tgaccgtgcc ctgtctgtgg
      301 atgagtattt ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga
      361 cgcaggccga caaaacggag ccctgccatg aatgggatat ggccggtatt cagccttctc
      421 ctgcacaggc acagaatatt cgcgaaaaaa cggaggcggt attccgtctg ttccggcccg
      481 tacatccggt tgtggccgta tcggcccgca ccggctggga actggatacg ctggtcagtg
      541 cgctcatgac agcgcttccc gaccatgccg ccagtcccct gatgacccga ctgcaggacg
      601 agctgcgcac ggaatctgtc cgcgctcagg cccgtgaaca gtttaccggt gcggtggacc
      661 ggatatttga cacggcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg
      721 ctgttcgtga cacggtggtc tctgttgccc gtgcggtatg gaactggatt ttcttctgaa
      781 cctgtcgtga ctgatgccct ccctgactct gagtctgctc acaaaagcac tgttttcgtt
      841 actgtctctc ttgtccgtgc aatagctcaa taatagaata aaacgatcaa tatctatttt
      901 atcgatcgtt tatatcgatc gataagctaa taataacctt tgtcagtaac atgcacagat
      961 acgtacagaa agacattcag ggaacaacag aaccacaatt cagaaactcc cacagccgga
     1021 cctccggcac tgtaaccctt tacctgccgg tatccacgtt tgtgggtacc ggctttttta
     1081 ttcaccctca atctaaggaa aagctgatga aacgacatct gaatacc
//
LOCUS       DAZFJF010000407         1118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFJF010000407 DAZFJF010000000
VERSION     DAZFJF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.410"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..625
                     /locus_tag="ACHKAU_005507"
     CDS             53..625
                     /locus_tag="ACHKAU_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5534321.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSASGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            686..>1118
                     /locus_tag="ACHKAU_005508"
     CDS             686..>1118
                     /locus_tag="ACHKAU_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5534322.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRN"
BASE COUNT          235 a          285 c          363 g          235 t
ORIGIN      
        1 catctgcctt tacggggatt tgcaacgatt tggccgccgc atcgaccttc gtgtgaaaac
       61 gggggctgaa gccatccgcg cactggccac acagctcccg gcgtttcgtc agaaactgag
      121 cgacggctgg tatcaggtac ggattgccgg gcgggacgtc agcgcgtccg ggttaacggc
      181 gcagttacat gagactctgc ctgatggcgc tgtgattcat attgttccca gagtcgccgg
      241 ggccaagtca ggtggcgtat tccagattgt cctgggggct gcagccattg ccggatcatt
      301 ctttaccgcc ggagccaccc ttgcagcatg gggggcagcc attggggccg gtggtatgac
      361 cggcatcctg ttttctctcg gtgccagtat ggtgctcggt ggtgtggcgc agatgctggc
      421 accgaaagcc agaactcccc gtacacagac aacggataac ggtaagcaga acacctattt
      481 ctcctcactg gataacatgg ttgcccaggg caatgttctg cctgttctgt acggggaaat
      541 gcgcgtgggg tctcgcgtgg tttctcagga gatcagcacg gcagacgaag gggacggtgg
      601 tcaggttgtg gtgattggtc gctgatgcaa aatgttttat gtgaaaccgc ctgcgggcgg
      661 ttttgtcgtt tatggagcgt gaggaatggg taaaggcagc agtaaggggc ataccccgcg
      721 cgaagcgaaa gataacctga aatccacgca gctgctgagt gtgattgatg ccatcagcga
      781 agggccgatt gaaggtccgg tggatggatt aaaaagcgtg ctgctgaaca gtacgccggt
      841 gctggacagt gaggggaata ccaacatctc cggcgtcacg gtggtgttcc gggcaggtga
      901 gcaggagcag acaccgccgg aaggttttga atcctccggc tccgagacgg tgctgggtac
      961 ggaagtgaaa tatgacacgc cgatcacccg caccatcacg tcggcaaaca tcgaccgtct
     1021 gcgctttacc ttcggtgtgc aggcactggt ggaaaccacc tcaaaggggg accggaatcc
     1081 gtcggaagtc cgcctgctgg ttcagataca gcgtaacg
//
LOCUS       DAZFJF010000408         1116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFJF010000408 DAZFJF010000000
VERSION     DAZFJF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.411"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1116
                     /locus_tag="ACHKAU_005509"
     CDS             <1..1116
                     /locus_tag="ACHKAU_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5534323.1"
                     /translation="FPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEALRRFEQMV
                     EEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESARQAAESAAS
                     AKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNASRDATTATEKARESA
                     ESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGDPGPAGPQGPKGDKGERGDTGPAGATG
                     ERGPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGETGA
                     AGPVGATGPQGPKGDPGETQIRFRLGPASIIETNSNGWFPGTDGALITGLTFLDPKDA
                     TQVQGLFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          289 a          280 c          394 g          153 t
ORIGIN      
        1 ttcccgccgt cacatgccgg gaccatcacc gtgtatgaag attctcaacc gggtacgctg
       61 aatgattttc tcggtgccat gacggaggat gatgcccgtc cggaggctct gcgtcgtttt
      121 gaacagatgg tggaagaagc agcgcgtcac gcagaggagg cgaagaagaa tgccggagaa
      181 gcagagacgt ccgcgaggaa tgccggcata tcagccagta aggcggaagc gagcgccgca
      241 aatgctgata cttcagcaga ggatgcatcg gagtcagccc ggcaggcggc agaaagtgca
      301 gcctctgcaa agaagtcaga ggaagcgtcc tcgtcctcag cctctgaggc cgctcaaaaa
      361 gccagtgagt cattacaaag tgcaacagat gccgagttgt caaaaaagac ggcagaaagt
      421 gcagccggta atgcatccag agacgcaacg acagcaacag aaaaagcccg ggagtcagca
      481 gaaagcgcac agtcagcgga acaaagcagg atagcggcgg aagaagccgt aaaccgaatc
      541 cccaccgtgg tggggccacc cgggccaaag ggagatccgg ggcccgcggg tcctcagggg
      601 ccgaagggag ataaaggaga gcgtggcgac accggcccgg caggggcaac tggtgaacgg
      661 gggccggcag gtgatgctgg tccggcaggc ccgcaggggc cgaaaggcga caggggagag
      721 cggggagaga ccggtctgac gggaaatgca ggtccacagg ggccaaaggg agataccggt
      781 gcggcaggcc cggctggccc acagggaccg aaaggagaaa caggtgcggc tggcccggtg
      841 ggggcaaccg gacctcaggg accaaagggc gacccggggg agacacaaat ccgttttcgt
      901 ctggggccgg cgagcattat tgagacaaac agcaatggct ggttcccggg tacagatggt
      961 gcgctcatca ccggactgac ctttcttgac cccaaagatg ccacacaggt tcaggggctg
     1021 tttcagcatt tgcaggtcag gtttggtgac gggccgtggc aggatgttaa ggggcttgat
     1081 gaagtgggca gtgatacagg cagaacagga gaatga
//
LOCUS       DAZFJF010000409         1102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFJF010000409 DAZFJF010000000
VERSION     DAZFJF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.412"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1102
                     /locus_tag="ACHKAU_005510"
     CDS             <1..>1102
                     /locus_tag="ACHKAU_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO5534324.1"
                     /translation="FADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTTLMGY
                     TQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTLPC
                     QYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHIHLST
                     TTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKRDWNR
                     FLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSH
                     RTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITETVRK
                     TAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTG"
BASE COUNT          257 a          255 c          345 g          245 t
ORIGIN      
        1 tttgccgaca tttttcagca gggaaaccgc tggcttaact ggctggagaa acaaccggaa
       61 ggttcagtgc gtccggtagt cattgagtct gtgacaaaaa tcatggcctg cgggaccacg
      121 ctgatggggt acacacagtg gtgctgttca tctccggact gcagccacat aaaaaaggtc
      181 tgcttccggt gtaaaagtcg ctcctgcccg cactgcggag tgaaggctgg cgcacagtgg
      241 atacagtatc tgctgagtct ggttcccgac tgtccgtggc agcatattgt gttcacactt
      301 ccctgccagt actggtccct ggtgttccac aaccggaggt tactggcaga gatgagccgc
      361 attgctgcgg atgtgataca ggaaatctgc cgccaggcag atgtggtgcc ggggatattc
      421 acggtgatcc acacatgggg acgtgaccag gagtggcatc cgcacattca cctgtcgaca
      481 acgaccggcg gcgtgacatc agaccacacc tggaaaaacc ttcattttta cgcccgtaag
      541 gtgatgagta tgtggcgtta ccggataacg cggttactgt cacggaaata tccggacctg
      601 gtgataccgg atgcgctggc agcagaagga agcagtaaac gggactggaa tcgcttcctg
      661 gacagtcatt accggcgggg ctggaatgtc aacgtatccc gggtgatgga taacgccaca
      721 catgtggcgg tgtacttcgg ctcttacctg aaaaaaccgc cggtgccgat gagccgtctg
      781 gagcactatg ctggtcagga tgaaattggt ctgcgttaca acagtcaccg gacaaaacgg
      841 gaagaatacc tggtgatgag tggtgatgag tttatggaaa ggttctcctg gcatgtggcg
      901 gataaggggt tccgtatggt gaggtactac ggtttcctga gtccggtgaa gcgccggtta
      961 ctggaagatg ttgtgtacgt cataacggag acggtgagaa agacggcgat gcaaatcagg
     1021 tggagaggga tgtatcagcg gttactgaag gttgacccgc tgaagtgcat cctgtgcgga
     1081 ggtcagatgc gttttacggg gc
//
LOCUS       DAZFJF010000410         1097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFJF010000410 DAZFJF010000000
VERSION     DAZFJF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.413"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..181
                     /locus_tag="ACHKAU_005511"
                     /pseudo
     CDS             1..181
                     /locus_tag="ACHKAU_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            406..897
                     /locus_tag="ACHKAU_005512"
     CDS             406..897
                     /locus_tag="ACHKAU_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5534325.1"
                     /translation="MSTETIEIFNNSDEWSNQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          430 a          177 c          161 g          329 t
ORIGIN      
        1 tagagaataa tgtagacctg aataaattca ctactatatg cacagacgtt ctatcccccc
       61 gcgtcaccag acataataaa gaaaaaaaac aaacgacatt ctacccttct caaaaatccc
      121 ctttttaatt ttcattgaaa aaatggcaat tgatactatg aatccaggtc atacacatta
      181 aggcatttat gtaatgtcgt aattaagata actaataagg tgaatattag taaaacaact
      241 aatgatgtta ttagtttgta gtgaacagta cttttaccaa taatgaaaaa tataccataa
      301 gcaatgcaag cttaaaactc ataatttaaa aaagataaat ataaaaatat caatgagtca
      361 atcaataata atttggcatc acgagacaca tcacagagga atattatgag cacagaaaca
      421 attgaaatat tcaataatag tgatgaatgg tcaaatcaac taaaacacgc attatcgaaa
      481 ggagaaaatc tggcattact acatggttta actcctgata tccttgatag aatatatgca
      541 tatgcattcg actaccatga aaagggtaat ataacggacg cagaaattta ttataaattt
      601 ttgtgcattt atgcgttcga aaatcatgag tatctaaaag attttgcatc agtatgtcag
      661 cccaaaaaga aatatcaaca agcatatgac ctttacaaac taagttacaa ttactccccg
      721 tatgatgact attcagttat ttatcgtatg ggtcaatgtc agattggggc taaaaatatc
      781 gataacgcaa tgcaatgttt ctatcacatt attaacaatt gtgaggatga tagtgttaag
      841 agtaaagcgc aggcatatat tgaactctta aacgataatt cagaagataa tggctaagca
      901 ttacaatcat ttggttaaat ttgctggtta tagtgcagtt aaccactata accagcacct
      961 cttatgtttt cttaaatcag catcttaaaa cagttaagag catactataa atcattattt
     1021 ttcaatgcgt tagagaacaa tctactttat caaaattgaa taaggctatt tcagattaca
     1081 tcactgaatt cctaaga
//
LOCUS       DAZFJF010000411         1088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFJF010000411 DAZFJF010000000
VERSION     DAZFJF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.414"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..336
                     /locus_tag="ACHKAU_005513"
     CDS             91..336
                     /locus_tag="ACHKAU_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534326.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            308..718
                     /locus_tag="ACHKAU_005514"
     CDS             308..718
                     /locus_tag="ACHKAU_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5534327.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            715..891
                     /locus_tag="ACHKAU_005515"
     CDS             715..891
                     /locus_tag="ACHKAU_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5534328.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            894..>1088
                     /locus_tag="ACHKAU_005516"
     CDS             894..>1088
                     /locus_tag="ACHKAU_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534329.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKN"
BASE COUNT          354 a          224 c          268 g          242 t
ORIGIN      
        1 tttgaggaat acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga
       61 aatggtgata ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg
      121 tggactttat ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg
      181 aatgacgact tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg
      241 cgtgacaaaa cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag
      301 aatttcgatg aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa
      361 cgccatcctc gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta
      421 caagcgcaac cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca
      481 ggttcaatgg tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg
      541 tcatgcagtg gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt
      601 caacattcgc gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta
      661 cacgacagca tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga
      721 cgacagcgac gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa
      781 cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac
      841 acggctcacc tgtgggatat ccggtggcta agacatcgtg cgaggaaatg acaatgcttt
      901 taattcaacc tggatttgga cttagcatca aaaaaggcca catgtttggt gagaaagagt
      961 cacaacgaaa aatggtgtct atccggttgc catttatcag tatttattgg ctaaacaggg
     1021 aggcaacaaa ttattggtat acatgcgcca gagcagcatt taacgaccct gactggtttg
     1081 tgaaaaac
//
LOCUS       DAZFJF010000412         1086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFJF010000412 DAZFJF010000000
VERSION     DAZFJF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.415"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..459)
                     /locus_tag="ACHKAU_005517"
     CDS             complement(<1..459)
                     /locus_tag="ACHKAU_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5534330.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDI"
     gene            complement(440..>1086)
                     /locus_tag="ACHKAU_005518"
     CDS             complement(440..>1086)
                     /locus_tag="ACHKAU_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5534331.1"
                     /translation="AAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIA
                     AGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLEEAIV
                     RRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEKEC
                     AKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          213 a          356 c          302 g          215 t
ORIGIN      
        1 tatgtcacgc acacgggcga tgatgtcagc gcagtcaaat gcccccgcca ccatcccgcc
       61 gggcgtgtcc atatcgagca gaatgccgtc caccatcgga tcgctggcag cctgttgcag
      121 acgggcgata atgccgttgt aaccggtcat ccccgagtac ggctgcagcg cccgcgtccg
      181 gctgaccagc gtgccggaca ccggcagcac ggcgatgccg ttcatgacct gataactgcg
      241 ggcctgtcgt ggtccgtcat catcaccgga taatgccagc gtcgcgagtg cctcctgggc
      301 agtcaggctg tcgccggaca ccgcatccgt caggcggctg atcccaagct ggcctgcaag
      361 cgcacaaaag aaaacccgcg cataggcggg ttcaagcatc agcggctcat taaaagccat
      421 gctggcaata tgcgggagat tacgcagctc tgctgtcact cttctcctcc tctgttgatt
      481 gtcgcagtcc ggattcaaat gccgcagccg cccaggcggg cggtttaaga ccggctgcgc
      541 ggcgctccat cgtttcacgg acctgctggg caaaaatttc ctgatagtcg tcaccgcgtt
      601 ttgcgcactc tttctcgtag gtactcagtc cggcttctat cagcatcacc gcttcctgta
      661 cttctttcag accatcgatg gccatacgac cggagcctat ccagtcacag ttcccccagg
      721 cgctgcgggc ttcctgaaag ctgaaacgcg cttttgaagg taacgtcacc acgcggcgaa
      781 cgatggcctc ttccagccag cacagaaaca tctggctcgc ctgacgggat gcgacgaatt
      841 ttcgccgccc cataaagtac gcccacgact cgttcgcact ggcccgtgcc gtggagtagc
      901 tcatctgggc gtaattccgg gaaagctgct catacgagac acccagtccg gcagcgatat
      961 accgcagcag tgactgctca aacacggagt agccgttatc cgtgtcctga gccgtctgca
     1021 ggttcagaga gtcacccggc atcaggtgcg gcacttttgc gcctcccagc cggaccggcg
     1081 cggcgg
//
LOCUS       DAZFJF010000413         1083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFJF010000413 DAZFJF010000000
VERSION     DAZFJF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.416"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            426..614
                     /gene="dicB"
                     /locus_tag="ACHKAU_005519"
     CDS             426..614
                     /gene="dicB"
                     /locus_tag="ACHKAU_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5534332.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            611..799
                     /locus_tag="ACHKAU_005520"
     CDS             611..799
                     /locus_tag="ACHKAU_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5534333.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            894..>1083
                     /locus_tag="ACHKAU_005521"
     CDS             894..>1083
                     /locus_tag="ACHKAU_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5534334.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCED"
BASE COUNT          289 a          240 c          259 g          295 t
ORIGIN      
        1 aaagcgtacg gcgaagctct ttcccttaga aggcttgtcg ttagatttct tcgaacgtgc
       61 gcttccggtt gtggcactcc gcgaaatggc gcggcggtaa gtatggcggg gttattcctt
      121 ccccattgag gacaccgggt tgtcaggttg accatacgct taagtgacaa ccccgctgca
      181 acgccctctg ttatcaattt tctggtgacg tttggcggta tcagttttac tccgtggctg
      241 ctctgccgcc ctttttaaag tgaattttgt gatgcggtga atgcggctaa gcgcacgcgg
      301 aacagttaaa accaaaaaca gtgttatggg tggattctct gtatccggcg ttaattgtta
      361 actggttaac gtcacctgga ggcaccaggc actgcatcac aaaattcatt gttgaggacg
      421 cgataatgga aacgttatta ccaaacgtta atacgtctga aggttgtttt gatattggtg
      481 ttctgctcag taaccgggag tttactgaag atgccattaa catgaggaaa tatgagcctt
      541 atctgctcaa tgataattcc atactttccc gaattgctct tcttgaactt ggtattttcg
      601 gagaacgtca atgacttcag catttgcact gatgatgacg gtttttctta taacgggtga
      661 atcacagaat gtgattaccg gaatttatgc cagtaaagaa tcctgcctcc aggcaagaga
      721 cgagcaaaaa atttctggtg aatgcctccc ggtaaaaaaa gtatcgctgt acctgaataa
      781 cgaaacaccg gctggataac cctccagcca tattaacacc ataccaacgg attaaaaatg
      841 ccagcaatgg cagggattcg ttcaccctga aatctgtaat gaggtaaaac aaaatgagta
      901 aagtctttat ttgcgccgcc attccggacg aacaggcaat aaaggaagaa ggtgcagtcg
      961 ctgtagccac tgccattgaa gccggtgatg aacgtcgcgc ccgcgcaaaa tttcactggc
     1021 aattcctgga acattatccg gctgctcagg actgcgctta taaatttctt gtttgcgagg
     1081 ata
//
LOCUS       DAZFJF010000414         1082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFJF010000414 DAZFJF010000000
VERSION     DAZFJF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.417"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            340..777
                     /locus_tag="ACHKAU_005522"
     CDS             340..777
                     /locus_tag="ACHKAU_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO5534335.1"
                     /translation="MCELINEASKYGKVNERKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTINEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            797..1082
                     /locus_tag="ACHKAU_005523"
                     /pseudo
     CDS             797..1082
                     /locus_tag="ACHKAU_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT          323 a          202 c          265 g          292 t
ORIGIN      
        1 gttatttaga ggaaataatt ttgtctggtt cataaattca tcttctttgt tgagaagatt
       61 aactgcctag gtgaaattat cctctatgac cgggacgctg caggccacct ggcagaagtc
      121 atcacgcagg aagacaagtc gctgtatccc tttgaaaaat ccggcaggct aatgggcatc
      181 ttcagcccag gcggcacatc acagcgaatt tgaaggcaaa tgcgaaaatt agtgatcttg
      241 aagttcatac agtattggta aaaacaggcg aagcttttcc aatgtgtaag aattttcaaa
      301 tttcaatatt tggcgctgtt gttacaagcg ggtgattata tgtgtgaatt aattaatgaa
      361 gcatcaaaat atgggaaagt caatgaaaga aagatggaca ttgatttgtg ctgtaaaaaa
      421 atagaggaag aaggatattc tgttttcgtt gcgttctatg attttataaa aaaatatggc
      481 ggtttatatg gctttcattc agcttacaaa gacaccacta taaatgaggc tttttttata
      541 gatccagaaa gggcctctaa tcgcattttt atagaaagag tcaatgatta tattgagaga
      601 gctggttaca atatgattcc tgttggcgag tgcagtaatg gtcatatcac attattatat
      661 ggggaaaata atgtgatata tggcggcttg gatgattatc tttggaagta tggggataat
      721 ttatcttcat caattgatat attgattaat ggcaaggagg cagttgaaat tccgtaaggc
      781 caacttgata tgacacctgg cacacaaccc cgacgaccgg atgtatatgc tgggcgggat
      841 gagtgacacc gccagcaacc gcatcacgtt tgagcgcgac agccagtacc ggatcacgtg
      901 tgtcagtcac accgacggga tccggcttaa actgacgtac catgccagcg gctacctgaa
      961 agccattcac cgcacggata acggcataca gacgctggcg acctacgaac aggatgcgcg
     1021 ggggcggctg accgaagcgg atgcgcggct ggactaccac ctgttttatg agtacgacgc
     1081 tg
//
LOCUS       DAZFJF010000415         1053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFJF010000415 DAZFJF010000000
VERSION     DAZFJF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.418"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..343
                     /gene="etgA"
                     /locus_tag="ACHKAU_005524"
     CDS             <1..343
                     /gene="etgA"
                     /locus_tag="ACHKAU_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO5534336.1"
                     /translation="WNESKNKNGIKSKINKNGTYDIGIMQINSSHLDLLSKFNISEDD
                     LLNDACINISVAGYILASNIKSRGNTWDAVGAYNAGYFNTPNAVELRRQYAMKIYKTY
                     TKLKNNEQIID"
     gene            complement(340..>1053)
                     /gene="escU"
                     /locus_tag="ACHKAU_005525"
     CDS             complement(340..>1053)
                     /gene="escU"
                     /locus_tag="ACHKAU_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO5534337.1"
                     /translation="FAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIITLI
                     FYFMGHSYANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGL
                     KKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAI
                     CLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDITLARSLYKNIHKGQYIT
                     EDFFEPVAQLIRIAIDLDY"
BASE COUNT          373 a          183 c          167 g          330 t
ORIGIN      
        1 atggaacgaa tcaaaaaaca aaaacggaat aaaaagtaaa ataaacaaaa acggaacgta
       61 tgatataggt ataatgcaaa taaattcatc tcatttagat ttattaagca aatttaatat
      121 atctgaagat gatttattga atgatgcctg tataaacata tctgttgctg gttatatctt
      181 agcctctaac ataaaatcca gggggaatac atgggatgca gttggtgcgt ataatgcagg
      241 ctactttaac acaccaaatg cagtagaact cagaagacaa tatgcaatga aaatatataa
      301 aacatacact aaattaaaaa acaatgaaca aattatcgat taataatcaa gatctattgc
      361 tatacgaatc aattgtgcta caggttcaaa aaagtcttct gttatgtatt gtcctttatg
      421 tatgttctta taaagacttc ttgccaaagt aatatcttca atcactggaa tatcatagag
      481 ttcagccagt ttaatgatct gtagagcttt ggcatctttt cctgtttcaa taactaaagg
      541 caatggagtc tccccgagtt tatagtatag gcaaatcgca atgtgagtcg ggtttttgac
      601 gatcacggtt gattttttga tgttattagc cagacttcca ctttgtatct cagaatgaag
      661 gcgacggcgc tcccctttaa tttcagggtt accatcagta tccttggctt ctcgtttcac
      721 ctcatcttta ctcattttca ttttcttcag tccctcatgc ttctggaacc agaaatcaaa
      781 tactgaaaag agtagatatc cgaataacac gcctttccat aaaagaaaaa caaaaaaggc
      841 aacaacgaca agagtttgat atgcgttcag tcttgtgaaa tttgcaaact catttgcata
      901 tgaatgcccc ataaagtaaa aaatgagagt aattatcact aacttaaata ctgacttaag
      961 cagctcaaaa atactcttta cagaaaaaat atttttcagg ttatttttta cactaatctt
     1021 ctgagccgat ggttttattt tttcaaccgc aaa
//
LOCUS       DAZFJF010000416         1050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFJF010000416 DAZFJF010000000
VERSION     DAZFJF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.419"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1031
                     /locus_tag="ACHKAU_005526"
     CDS             <1..1031
                     /locus_tag="ACHKAU_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5534338.1"
                     /translation="PLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHGVVFPAELVGL
                     LDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLA
                     LISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRHRYYDPLQ
                     GRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAAN
                     SITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVIGKNHEAAGD
                     RQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKAKQ
                     GSSDASNY"
BASE COUNT          289 a          212 c          315 g          234 t
ORIGIN      
        1 cgccgctcat ccgtatcgaa acagacaacg gtgaacggga gaaagcgcag tgccgcagcc
       61 tggcggagaa gatacagcag gaagggagcg aagacggtca cggtgtggtg ttcccggctg
      121 aactggtggg gctgctggac aggctggagg gggaaatccg ggcaaactgc gtgagcagtg
      181 aaagccgcca gtggcttgcg cagtgcgggc tgacggtgga gcggctggcg gcacagatag
      241 agccggttta tttgcctgag cgaaaaatac atctttatca ctgtgaccac cggggcctgc
      301 cgctggcgct tatcagtgaa gacggcaata cggcgtggag cgcggaatat gatgaatggg
      361 gcaaccagct taatgaggag aacccgcatc acctgcacca gccgtaccgg ctgccggggc
      421 agcagtatga taaggagtcg gggctgtact ataaccgtca ccggtactac gatccgttgc
      481 aggggcggta tatcaccccg gacccgattg ggttgagagg tggatggaat atgtatcagt
      541 atccgttgaa tcccatacaa gtgatagacc caatggggtt agatgcgatt gagaatatga
      601 catcaggtgg actaatttat gccgtatctg gtgtacctgg attgattgct gcaaacagca
      661 ttactaacag tgcttaccag ttcggttatg atatggatgc tattgttggc ggagctcata
      721 atggggccgc cgatgcaatg agacattgtt acttgatgtg tcgaatgact aagacatttg
      781 gatcaacaat agctgacgtg ataggtaaaa atcatgaggc ggcaggggat agacaaggtc
      841 agccagctaa agaaagaatc atggatctta aaaataacac tgtcggtatt gcttgtggcg
      901 atttttctgc caaatgtagc gatgcatgta ttgaaaaata taacactggg caactcttcg
      961 ggttagatgg tataaaagca gataatccaa taaaagcaaa gcaagggagt tcagatgctt
     1021 caaattatta gaggcaaact tgtcattttt
//
LOCUS       DAZFJF010000417         1034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFJF010000417 DAZFJF010000000
VERSION     DAZFJF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.420"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..496)
                     /gene="yhaB"
                     /locus_tag="ACHKAU_005527"
     CDS             complement(<1..496)
                     /gene="yhaB"
                     /locus_tag="ACHKAU_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO5534339.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQ"
     gene            complement(752..970)
                     /gene="tdcR"
                     /locus_tag="ACHKAU_005528"
     CDS             complement(752..970)
                     /gene="tdcR"
                     /locus_tag="ACHKAU_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO5534340.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          311 a          189 c          193 g          341 t
ORIGIN      
        1 tttgccaaca atctgaagta atgctggttt tattaaagtg cacaaggggt gttaagatgg
       61 cttcttttcg ggtgaatttt gcacaatact tagaaatcgt ctccttaata cattccagct
      121 cttttttatt gacggctgag caagtacatt gttcgccaaa aggtgcaaaa gtgcctacat
      181 ctaatgtttg tatgatataa tcatcatgtt cccctgtaga aatatctatg atgaatggac
      241 tcatattttc cggttggatt cggatcatta cagaaggaac atcgccttga tagaaattca
      301 atgcagtaat tgcagataat actctccgca gttcattact tatactgtaa attttattaa
      361 cgtcattggt ggagacaatt tctgcaaact ttctttttac cgtccaataa tcaatatttc
      421 tattgtgatt gttaaccact gcgtgcattg gcgggattga agggtgaaaa atatgtgcaa
      481 ttgggaagcc tttcattgat gcctgccttg tcgtcatgac aaacaataat agatgcttat
      541 tttcctcgca gggttctttc gcactatcaa aaaacgctca tttacccggc gttacagtaa
      601 acgaagcaac attctgtggc ctacgacgat caaaagctct gtgaaaattt cccttgttgt
      661 ctttgtctga ccttatcgcg gcgaccagca attattcgtt ttgtgatcct caacacaaaa
      721 tgctattctt cgcctatgag gggagaagaa tttatggaaa acgattgcat tcacaaaatg
      781 acgtcctgtc agaagagaaa aagttgacgt taatcaattg ttcgagatga tcaatacctg
      841 tattcgaaat tttaaaatat tgataaaata aatgtttgtt ctccaacaaa tagcgtgaaa
      901 aaatgtgacg tgccgcagta tttatcgctg aaaatggttt atttataata aaatttgaga
      961 acttgctcat taaatttaac tcaaattttg cctggtaatt atccggtaat tgcttgaaat
     1021 atggtctcag cccc
//
LOCUS       DAZFJF010000418         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFJF010000418 DAZFJF010000000
VERSION     DAZFJF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.421"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1026
                     /locus_tag="ACHKAU_005529"
     CDS             <1..>1026
                     /locus_tag="ACHKAU_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5534341.1"
                     /translation="RISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITE
                     YLTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFY
                     FLFTDKDSEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSE
                     QPEPVKVTADEVKKIMQAANISQPDANQLLAASRGEFVAGISDPNDPKWVKGIETRDS
                     VNQNQQETEQNDQKAEQNSPNTQQNEPETKLPEPVVQQEVEKVCTACGQTGGGNCPDC
                     GAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNETGETADH
                     PIKVNGH"
BASE COUNT          343 a          248 c          258 g          177 t
ORIGIN      
        1 gcgtatctcg gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat
       61 ttttggcctt gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa
      121 atacattact gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa gaaatcagag
      181 cgcaactgat actctctcgt cgccagtacc agaaactgat gcagtggaaa cggcaattcc
      241 ggacaacgaa aaaaccgaat gcaaagtgga agtcgaacca tctgtagagc gtgaagggcc
      301 gttctacttc ctcttcaccg acaaggatag cgaaaaatac ggtcgcgcaa acaaactttc
      361 tggtctggat aaggcactgg ctgcaggggc tactgaaatc acgaaagaag aatatttcgc
      421 ccgaaaaaat ggcacataca caggtttacc gcaaaatgca aataccgcac aaaattctga
      481 acaaccagaa ccggtaaaag ttaccgctga cgaagtaaag aaaattatgc aggcagccaa
      541 tatcagccag cctgacgcca atcagttgct cgccgcatca cgtggtgaat ttgttgcagg
      601 gattagcgac ccgaatgatc cgaaatgggt gaaggggatt gaaacccgcg attctgtgaa
      661 ccagaaccag caagaaacgg aacagaacga ccagaaagcg gaacaaaaca gcccaaatac
      721 gcaacaaaac gagccagaaa cgaaactacc tgaaccagta gtgcaacagg aagtggaaaa
      781 agtctgcacc gcctgcggtc agaccggcgg cggcaactgc cctgattgtg gcgcggtgat
      841 gggcgacgct acataccagg aaacattcga tgaagagtat caggttgaag ttcaggaaga
      901 tgatccggag gaaatggaag gcgctgaaca tccacacaag gagaacactg gcggcaatca
      961 gcatcacgat agcgataatg aaactggcga gacggcagat cacccaatta aggtgaacgg
     1021 tcatca
//
LOCUS       DAZFJF010000419         1018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFJF010000419 DAZFJF010000000
VERSION     DAZFJF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.422"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..438
                     /locus_tag="ACHKAU_005530"
     CDS             <1..438
                     /locus_tag="ACHKAU_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5534342.1"
                     /translation="PGAPISNKRGQLDLDAWRDFYISYLRRSKNDVPDGDSEDDYEEK
                     LLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQ
                     FPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            410..>1018
                     /locus_tag="ACHKAU_005531"
     CDS             410..>1018
                     /locus_tag="ACHKAU_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5534343.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEG"
BASE COUNT          274 a          226 c          268 g          250 t
ORIGIN      
        1 ccgggtgccc cgatatcaaa taagcgaggg caactggatc tggatgcctg gcgcgatttt
       61 tacatatcgt atctgaggag aagtaaaaac gatgtgcctg atggcgatag cgaagacgac
      121 tatgaggaaa aattgcttat tgccagatgg gaactgacag cagaacaggc tgttacacag
      181 cagttaaaaa atgaggtgtc aaaaggaaaa cttattgaca ccgggttctg tatttttgcc
      241 ctcagtaagc tggcaatggc gttatccagt acgcttgatt ccatcccttt atccatgcaa
      301 cgacagtttc ctgatttaac accgcgccat cttgaccatc tgaaaaccct tattgcgaag
      361 ggggcaaatc agtgtgcgcg ggcgggggat aaattaccgg atttactcga tgaatatatc
      421 agagcaacaa ctgaataata tgatgagtgc tgtcacaaca gcattacagc ccctgataag
      481 ggcattgccg gtgacgccag ttgaatgggc tgatcaaaat tattatctgc ctaaagaatc
      541 ttcatatggt gagggagaat ggaaaacgct gccattccag atcgccatca tgaacagcat
      601 ggggaatgat cagatccgca ctgttaatct gattaaatct gcccgtgttg gctatacaaa
      661 gatgttgctg ggggtggtcg ggtattttat tgagcataaa tcccgaaaca gtctgctttt
      721 tcagcccacg gattctgccg ctgaagattt tatgaagtct cacgtggagg cgacgattcg
      781 gaacgtgcca tgcctgaaag acctttcccc atggctgggg cgtaaacatc gtgacaatac
      841 tctcacgctg aaacgctttt catcgggcgt cggtttctgg tgcctgggcg gcgctgccgc
      901 caaaaactac cgtgaaaaat ccgtggacgt ggtctgctat gacgaacttt cctcgttcga
      961 gccggatgtc gaaaaagagg gctcgccaac cctgctgggg gataagcgta ttgagggg
//
LOCUS       DAZFJF010000420         1018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFJF010000420 DAZFJF010000000
VERSION     DAZFJF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.423"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..438
                     /locus_tag="ACHKAU_005532"
     CDS             <1..438
                     /locus_tag="ACHKAU_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5534344.1"
                     /translation="PGAPVSNKRGQLDLDAWRDFYISYLRRSKNDVPDGDSEEDYEEK
                     LLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQ
                     FPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            410..>1018
                     /locus_tag="ACHKAU_005533"
     CDS             410..>1018
                     /locus_tag="ACHKAU_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5534345.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEG"
BASE COUNT          270 a          227 c          268 g          253 t
ORIGIN      
        1 ccgggtgccc cggtctcgaa taagcgaggg caactggatc tggatgcctg gcgcgatttt
       61 tacatatcgt atctgagaag aagtaaaaac gatgtgcctg atggcgatag cgaagaagac
      121 tatgaagaga aattgcttat tgccagatgg gaactgacag cagaacaggc tgttacacag
      181 cagttaaaaa atgaggtgtc aaaaggaaaa ctgattgaca ccgggttctg tatttttgcc
      241 ctcagtaagc tggcaatggc gttatccagt acgcttgatt ccatcccttt atccatgcag
      301 cgacagtttc ctgatttaac accgcgccat cttgaccatc tgaaaaccct tattgctaag
      361 ggggcaaatc agtgtgcgcg ggcaggggat aaattaccgg atttacttga tgaatatatc
      421 agagcaacaa ctgaataata tgatgagcgc tgtcacaact gcattacagc ccctgataag
      481 ggcattgccg gtgacgccag ttgaatgggc tgatcaaaat tattatctgc ctaaagaatc
      541 ttcatatggt gagggcgaat ggaaaacgct gccattccag atcgccatca tgaactgcat
      601 ggggaatgat cagatccgca ctgttaatct gattaaatct gcccgtgttg gctatacaaa
      661 gatgttgctg ggggtggtcg ggtattttat tgagcataaa tcccgaaaca gtctgctttt
      721 tcagcccacg gattctgccg ctgaagattt tatgaagtct cacgtggagg cgacgattcg
      781 gaacgtgcca tgcctgaaag acctttcccc atggctgggt cgtaaacatc gtgacaatac
      841 tctcacgctg aaacgctttt catcgggcgt cggtttctgg tgcctgggcg gcgctgccgc
      901 caaaaactac cgtgaaaaat ccgtggacgt ggtctgctat gacgaacttt cctcgttcga
      961 gccggatgtc gaaaaagagg gctcgccaac cctgctgggg gataagcgta ttgagggg
//
LOCUS       DAZFJF010000421         1015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFJF010000421 DAZFJF010000000
VERSION     DAZFJF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.424"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..859
                     /locus_tag="ACHKAU_005534"
     CDS             <1..859
                     /locus_tag="ACHKAU_005534"
                     /inference="COORDINATES: protein motif:HMM:NF016926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HFO5534346.1"
                     /translation="IYKAEEMDKDNLFNDLNKLNGYLDSLDERGLILSLAAFSEDALG
                     KMLLTFMLDNKASKELIEGFNAPLGTFSSRIKACFSLGLITEGQYKDLELLRKIRNKF
                     SHSWENISIEDQDISQQIKALSFSRIDFECPKDNYQKIKKSISCLLIEIKITTSQIKK
                     KHLKARLVGSNVNIGFSGKYEEQVNDIKKNIESIKNDLTSHDKNIKSFAVHTANLLIE
                     RLSYVQFNHDDLDVFSDQLVDILEIKYQLLNLLGINGVTDLSQKEKEKLKKSFIERIT
                     IQTSNVSKK"
BASE COUNT          384 a          162 c          166 g          303 t
ORIGIN      
        1 tatttataag gcagaagaaa tggataaaga taatttattt aatgatctaa acaaactgaa
       61 cggttatttg gacagcctag atgaaagagg cctaatattg tctctagcgg ctttctctga
      121 agacgcctta ggtaaaatgc ttttgacgtt catgctcgat aacaaagcgt ctaaagaatt
      181 aatcgaaggg tttaatgccc cccttggaac attttcttca cgaataaaag cctgtttttc
      241 cttgggacta ataacagagg gtcaatacaa agatcttgaa cttcttagaa aaataagaaa
      301 taaattctct cacagctggg aaaacatttc aattgaggat caggatattt ctcagcaaat
      361 taaagcgtta agcttcagtc gtattgattt tgaatgccca aaggataact atcaaaaaat
      421 aaaaaaatcc atatcgtgtt tactaattga aattaagata accacatctc aaatcaaaaa
      481 gaaacacctc aaagcaaggt tagttggttc aaatgttaac ataggattct ccggtaagta
      541 tgaggaacaa gttaatgata ttaagaagaa catagaatcc ataaaaaatg atttaacttc
      601 acatgataaa aatataaaat catttgcagt acatactgct aatttactca ttgaacgttt
      661 atcatatgtg caatttaacc atgatgacct agatgttttt tctgaccaat tagtagatat
      721 cctagaaata aaataccaat tgcttaattt attaggaatt aatggagtta cagatttaag
      781 tcaaaaggaa aaggaaaagt tgaaaaaaag ttttatagaa agaattacga tccagactag
      841 taatgtatct aaaaaatgat aaaaccacac cgactaaaac tgtaatgaac agagttagtc
      901 ggctggtttc tataacaaaa gccataccgt ttgaacactt tttgtttttt cactatcatc
      961 tcagaatggc cattgcacct cgctctaaaa taatgcccct tggttgaggt tttcc
//
LOCUS       DAZFJF010000422         1005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFJF010000422 DAZFJF010000000
VERSION     DAZFJF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.425"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..114)
                     /locus_tag="ACHKAU_005535"
     CDS             complement(<1..114)
                     /locus_tag="ACHKAU_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5534347.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQF"
     gene            complement(286..516)
                     /gene="ynfN"
                     /locus_tag="ACHKAU_005536"
     CDS             complement(286..516)
                     /gene="ynfN"
                     /locus_tag="ACHKAU_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO5534348.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(521..586)
                     /gene="ynfR"
                     /locus_tag="ACHKAU_005537"
     CDS             complement(521..586)
                     /gene="ynfR"
                     /locus_tag="ACHKAU_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFO5534349.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(589..777)
                     /gene="ynfQ"
                     /locus_tag="ACHKAU_005538"
     CDS             complement(589..777)
                     /gene="ynfQ"
                     /locus_tag="ACHKAU_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5534350.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(788..1000)
                     /gene="cspI"
                     /locus_tag="ACHKAU_005539"
     CDS             complement(788..1000)
                     /gene="cspI"
                     /locus_tag="ACHKAU_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO5534351.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          314 a          197 c          166 g          328 t
ORIGIN      
        1 aaactgaata ctggtaactt ctttcccggt ctttttctta agttcaataa ttttttttgt
       61 tatatattca gaaatatctg cttctgcttt tgtttttaag ttttcaatat tcatcatttc
      121 ctcttttagt ctgttatgac tttccagtta cacagtaagt cgattatatg gtgcaaacgt
      181 gtaaaagata agatgaaaca tcgcaataat caacatacga tagtctaaat tttacacaaa
      241 cagacaaaga gaattttcct gaattatcaa tacaatagca tcaaatcaac tcaagagcct
      301 tattgctgct tccagaattt cttctgaagt aacatgtcga tccgcggcta cataaatgac
      361 tttatgatct ccggtcagag atggaaaccc tgcggccatt acagtaaggt gtgttttttc
      421 gccatttgga tattcacgca tgatggtgtt aactccagtc atcgctggca ctaccactgc
      481 tggttcagag ttaaaaaaaa ctatgatttt tttcatgatg ttaccgtagt atgtgagtat
      541 ccatcgaata gataccaagc aaaaaagctc ccgaaggagc cttcattttc acttttttaa
      601 atccaacgac agacggctgg catttaagta ttgtgaaata ttatcaaatg taatcatcat
      661 tgatttacaa aagatacatt ttgccccgaa aggattcatg tcagaaacat caaaagatga
      721 tgttctatac tgggaaccat gacaacacgg gcatctaaag tgaatatggt ttgtaatatt
      781 gtctacctca aagcgccact acatgaacag cggcaggacc tttaggtccg ttctcaatac
      841 caaattcaac ttcctgattc tcagttaatg ttttgaaatc gttgctctga attgctgaga
      901 aatggacaaa cacatctttg ctgccatctt tcggcgtgat gaaaccaaaa cctttttcag
      961 ggttaaacca tttcactaaa ccagtcattt tgttagacat aatta
//
LOCUS       DAZFJF010000423         1005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFJF010000423 DAZFJF010000000
VERSION     DAZFJF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.426"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..431
                     /locus_tag="ACHKAU_005540"
                     /pseudo
     CDS             1..431
                     /locus_tag="ACHKAU_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(450..1005)
                     /locus_tag="ACHKAU_005541"
                     /pseudo
     CDS             complement(450..1005)
                     /locus_tag="ACHKAU_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          339 a          188 c          165 g          313 t
ORIGIN      
        1 agcaactctt gctctaatga agcaggaaca atgttcagaa tcgaaccaaa tttaattaaa
       61 gctatcgctc ttgttgaaag caatctaaaa aaggatagca taggcaagaa cagagataag
      121 aataacaata taaagagttt agattattgg ttaatgcaga taaaccagat gcatattccc
      181 ctgttaaaaa aacgtggaat aataaaagat gaacgagatt tgttagataa cccttgtctg
      241 aacattaaaa taggaaccga gattttatat aaccattttt cacgttgtgg agtaacgtgg
      301 caatgcctgg ggacttataa tgcgggtttt gcaatggata accaaaaaaa gagacagcaa
      361 tatgccccaa aatatacatt gtatatacca ggcttaatga actagacaat cgtaaggctt
      421 cagcaaaatg agcctaaagc ctcttttttt tatatagtgc aaacacccat acgtaaagct
      481 gttttgatca actcgatacc actgtgaaca tctaatttct tcatgatatt gagtctatga
      541 gtttcgactg tttttctgct taactgcaag aaattagcga tttctttgtt tgttttacca
      601 tcagctatga gttgtaatat ttctgactca cgatttgtaa gttgattatc agaggaatac
      661 ctggaagata ttttttcaca atccatatgt acactatcaa aataagtgta accattacta
      721 atcgaatcaa tagcacgatt tagtttcgcc gggtgactgg ttttccatac gcatcctttc
      781 gcacctgctt caagcaattt tattgaatca atatacggca tctttgctac aaatgtaata
      841 atatgagcat ctatcttatg cgatttaacc cattttacga gttcggaccc catcattcca
      901 cctgcaaatt ctcctaaaaa aaatatttag gctgtagatt tgttacactc cgctatagct
      961 ttcttcagat ctttatagcc cccaacaacc tcatataaat cagga
//
LOCUS       DAZFJF010000424         1002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFJF010000424 DAZFJF010000000
VERSION     DAZFJF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.427"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..134
                     /locus_tag="ACHKAU_005542"
     CDS             <1..134
                     /locus_tag="ACHKAU_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534352.1"
                     /translation="LSKRERYSEKWQRRTHSVWRHRHWHVSAFTGIYNDLAAASACM"
     gene            131..712
                     /locus_tag="ACHKAU_005543"
     CDS             131..712
                     /locus_tag="ACHKAU_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5534353.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
BASE COUNT          239 a          237 c          319 g          207 t
ORIGIN      
        1 aactgagtaa acgggagagg tattctgaaa aatggcaacg acgaacgcat tctgtctggc
       61 gtcaccgcca ctggcacgta tctgccttca cggggattta caacgatttg gccgccgcct
      121 cagcctgtat gtgaacacgg cagcggaggc catccgtgcc ctgtcgctgc aggtgccggg
      181 attccgccgt cagatgaacg aaggctggta ccagatacgt attgctggtg aggacacggc
      241 accggaggcg gtgtatgccc gccttcacga acaactgggt gagggggcgg tcatccatat
      301 tgtgccgcga ctggccggtg cagggaaagg cggactgcag attgtgctgg gggcggcagc
      361 catcgtgggc tctttcttca cggccggagc atcgatggca gcctggggtg cagcgctgag
      421 tgccggcggt ttttctgcca ccacgatgct gttttcactg ggtgccagca tgatactggg
      481 tggtgtggct cagatgctgg ccccgaaggc taaagtaccg gagtacaaaa gcacggataa
      541 cggcagacag aacacgtact tttcctcgct ggataacatg attgcacagg ggaacccgat
      601 gccggtgcct tacggtgaaa tgctggttgg ctcacggcga atctcccagg acatcagtac
      661 ccgtgatgaa ggcggtgacg ggaaggtggt ggttatcggg cggcaggcat aaaagcgaaa
      721 aaatcccgca gtgaccgaag gctgcgggaa cagaaaatga agattaacca cagggagttt
      781 tgtttttatt ggcccgaaaa aactgtaacg cccgggaatg atatctgcca cgggggcgta
      841 cagaaaatgt gaagatattc agaattttta ttcagtcatg atacaggcat cctccgggat
      901 gcctgttgtt tttgtgcgta acagttatca cagtaaaggg tgagacaatg ggcaaaggtg
      961 gcggcaaggc gcacacgccg gttgaggcaa aggacaatct ta
//
LOCUS       DAZFJF010000425          988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFJF010000425 DAZFJF010000000
VERSION     DAZFJF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.428"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..96
                     /locus_tag="ACHKAU_005544"
     CDS             <1..96
                     /locus_tag="ACHKAU_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5534354.1"
                     /translation="FNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            248..>988
                     /locus_tag="ACHKAU_005545"
     CDS             248..>988
                     /locus_tag="ACHKAU_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534355.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLGMYEEKYRDYRDKFNEFSAYILQMKLAINKL
                     DSELNKLEPSLSAKNHLEKNQGIINSKLT"
BASE COUNT          346 a          157 c          202 g          283 t
ORIGIN      
        1 tttaattata tcgaatgtga ttacaatcgg tggcggcggc acagttggtg tggcggcctc
       61 agtccggaac aatttgaaaa caagaacctc gcttaggcct gtgtccatat tacgtgggta
      121 ggatcacgca ggggtcggcc catttgagct tcagatagga acgtttgaat tcctttcaca
      181 aaatacatca catactgaac gtgaacattc ccactttgat cttaaggcca aaaagtatgc
      241 aggactaatg atttttaggg atagcttgag agtattacct tatggtcgag tagataatga
      301 tttcttccag atagaagaaa gacgttcatg gaatgcaggg cgatattatt ggtctaatag
      361 aaggattttt ggttatattg gaattactca atccagtaat aaagagctga aagataagtc
      421 ggggagagag ggattcataa gaaaccaggc tgcaagagaa ctgaaaacta ttatatctaa
      481 tttgttaact gaacttgctg atagattttt tggttcacgt tctgatgacc gtaaagagct
      541 tttagaacaa gttaagcgtg aaaaagagtt aagaaaatct gctcaacaac aagctcgaaa
      601 atcaacacaa aaaagttttt cagaagcttt gaagaatcag acaccagttc ttgatgcttc
      661 cttggaggct gttaaaaggc tgaaaactaa gcttgataaa actgatggtt cgttagattt
      721 aaactatctt aaaattatag acagtgatct tacaaactta gatgcgttgc gcagtgaaat
      781 taaaacgcct atcaaacctc caaaacttgg aatgtatgaa gagaaatata gagactacag
      841 agataaattt aatgagttct ctgcgtatat tctacaaatg aagttagcaa ttaataaact
      901 tgattctgaa ttaaataaac ttgagccttc attgtcagcg aaaaatcacc ttgaaaaaaa
      961 tcaaggtatt attaattcta aactaact
//
LOCUS       DAZFJF010000426          986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFJF010000426 DAZFJF010000000
VERSION     DAZFJF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.429"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..468
                     /locus_tag="ACHKAU_005546"
     CDS             <1..468
                     /locus_tag="ACHKAU_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534356.1"
                     /translation="AYNVEVVFDPPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKY
                     EQIKDQIYNINISWASKPKSNDRETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIR
                     EDWKPIAQGAKKVKADVYTSSDRNEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            complement(517..>986)
                     /locus_tag="ACHKAU_005547"
     CDS             complement(517..>986)
                     /locus_tag="ACHKAU_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534357.1"
                     /translation="SIFKKHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSRSHF
                     ERALQLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIHSAM
                     KGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
BASE COUNT          346 a          174 c          191 g          275 t
ORIGIN      
        1 gcctataatg ttgaggttgt gtttgatcct ccactagtaa atgcggagca tagagaaaaa
       61 agtgagattc cgtttagtaa ggtaagcgtg ttaaaaaatg ggcaatcact taccagcaat
      121 ctctgtaagt atgaacaaat caaagatcaa atttataata ttaatataag ctgggcaagc
      181 aaacctaaat caaacgatag agaaacaaat gaatatgtgt atgacatggc gacatttgaa
      241 ggaataagtt atctaggagc gagaagccca ttgacgcaaa ttgcagaaca aattaaaggt
      301 ataagagagg attggaaacc tattgcacaa ggagctaaaa aagtaaaagc agacgtatat
      361 acttcaagcg atagaaacga agaacgcacg tatctgcaag agcaacacga tttggcaata
      421 aaaaggagag atgagaaaag agaaaaaaga ttagagtctg gtgaataatt ttaaagggag
      481 tgggtaacta acccactcgt aactataaac ctgtaattaa tcacttattt ttgacaacag
      541 ataattactg aacgcactgc aagtgactaa cataaattta gcttcagcta aggtaggatt
      601 tacatcttct tctgttagcg catgacgtat tccaccctga tcacttgtat aaccatagag
      661 ctgactaaaa gcgcctttca ttgcagagtg tatatatcct ttttcctcta tagctttaag
      721 acaagccccc aaggttcctt tatcattgcc cgtgattttc ctgcataaag attcaattgc
      781 agagatagac tctttaatcg agtttctgta gtctggctgc tctctatccg tcattagttg
      841 taacgccctt tcgaaatggc tacgcgatga atcagtgcca ttatcaactg cgttctgaac
      901 actttcaatt tcgttatcat ttgaaatagg agtaatacaa ccatttatta tggtataacc
      961 aacgccatgc tttttaaaga tggaat
//
LOCUS       DAZFJF010000427          973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFJF010000427 DAZFJF010000000
VERSION     DAZFJF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.430"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            520..973
                     /locus_tag="ACHKAU_005548"
                     /pseudo
     CDS             520..973
                     /locus_tag="ACHKAU_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          281 a          192 c          204 g          296 t
ORIGIN      
        1 ggtcgatgac ttcttccggc gaatactgat agaaacattg aaatattgta agagaaagcg
       61 ttcaaaattc tgtggcacgt gaaaaattct acagcgtcat caccttattc agtcgacctt
      121 caaaataaac gtttaacaga gacaatcacc atttggattg tccgctgagg aggcgaacaa
      181 tttcctagac agctttatca tgtcagccca cattgttggc gctggcaata ctaaggacaa
      241 acgatgggga agtagctttt tagcgttatt ttcgggagta aaaaacgtgg tatctttccg
      301 tatgtcagtt gtatcttgct ggtattcgta gtcgtgataa ggcatacaac gatatttatc
      361 ttgacgttag tgaagaataa gttgttttgt gatctatgtt aatctgggat tacaatccgg
      421 gcttgataca tcgcatcacg acttttattc tcttcagaat agaatatgtt actccccgct
      481 ttacaggctc aggatttgaa ttacaaatta tacttttaag tgaacaataa ccaagggtat
      541 ttcttatttc ctgaaaccag gcttggccca tttcgggatg agtttgaaaa aactcttgac
      601 gcatacgata gtgatgaaat aaacgaaaca aattacgtca acaaactcag gcgtcttgcc
      661 tgacatgctc ctgacatcat tgatattcac gctcatctgg catatgcatt tgcgctcttt
      721 atgctgcaat tctggcgaat gttcatttac agcgtcatca ggatgccgtt atactcattg
      781 ataaaatact aacatataac cctgagaata atcagggaac acgctggtta ctgggggcgg
      841 aattgttacg tgctggcaag catggcaggg ctctgtacgt attgacagga catgtcaatg
      901 aatttccccc ctactggtac gagcttgctc ttttacattt tatgaatgag gcatatatta
      961 atgctgcaac ggc
//
LOCUS       DAZFJF010000428          969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFJF010000428 DAZFJF010000000
VERSION     DAZFJF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.431"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..947
                     /locus_tag="ACHKAU_005549"
                     /pseudo
     CDS             1..947
                     /locus_tag="ACHKAU_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          284 a          225 c          252 g          208 t
ORIGIN      
        1 cgtaaggcgt taaatgaagc tcgccttgag cgagatatat taaaaaaagc aacagcgtat
       61 tttgcacagg agtcgctgaa aaatacgcgt taatcgaaca atggcgacaa caatttccca
      121 ttgaagcgat gtgtcaggta tttggtgtat ccaggagcgg ttattacaac tgggtacagc
      181 atgaaccctc agacagaaaa caaagtgatg agcggctaaa actggagatt aaggtggcac
      241 atatccgcac tcgcgaaaca tatggaaccc ggcggctcca gacggagctg gcagagaatg
      301 gcatcatcgt tggtcgtgac cgactggcac gtcttcgtaa ggagctaagg ctacgctgta
      361 agcagaaacg caagttcaga gcgactacga actcgaacca caatctgcca gttgcgccaa
      421 atctgctgaa ccagacgttc gctcctacag caccaaatca ggtctgggtg gcggacctga
      481 cgtatgttgc cacacaggag ggatggttgt acctcgctgg catcaaagat gtttatacgt
      541 gcgaaattgt cggctacgcc atgggagagc gcatgacaaa agagctgaca ggtaaagctc
      601 tgtttatggc gctcaggagc cagcgcccac ctgccgggct aatccaccac tctgatcgag
      661 gttcacagta ctgcgcatac gattaccggg tcatacagga gcagtttggt ctgaaaacat
      721 caatgtcgcg taaaggtaac tgttacgaca acgctccgat ggaaagcttc tggggaacgc
      781 tgaaaaatga gagcctgagc cactatcgtt ttaataaccg ggatgaagcc atctcagtaa
      841 tacgggaata cattgagatt ttctacaatc gtcagcgtcg tcactctcgt ctggggaata
      901 tctccccggc agccttcagg gaaaaatatc atcagatggc tgcttaaaaa aagaacaaat
      961 ggtagtgtc
//
LOCUS       DAZFJF010000429          958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFJF010000429 DAZFJF010000000
VERSION     DAZFJF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.432"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..882
                     /locus_tag="ACHKAU_005550"
     CDS             <1..882
                     /locus_tag="ACHKAU_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5534358.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     RFISPFEYAKINNPLKQ"
BASE COUNT          267 a          229 c          219 g          243 t
ORIGIN      
        1 ttccccttta tcggcaatcg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
      181 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
      241 ctgcctgaac ttttgtcgcg tattggcagt tacaaggggc aacctgtcac ccagcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg atccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggttgtag ccatactggc agaacttcag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccacg taaaccaatc agtgaaaaca ccgtaaataa agccctgagg
      601 gttatggggt atgacacaac ccaggatgtc tgtggtcatg gattccgggc gatggcatgt
      661 agtgcgttaa tagaatcagg tttgtggtcc cgcgatgcag ttgaacgtca gatgagccat
      721 caggaacgta acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt ctggatgcaa acagagaaag atttatcagt
      841 ccatttgaat atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc
      901 cgggaaccta ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaac
//
LOCUS       DAZFJF010000430          953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFJF010000430 DAZFJF010000000
VERSION     DAZFJF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.433"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHKAU_005551"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHKAU_005551"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO5534359.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          255 a          237 c          209 g          252 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa
       61 tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgttgccag tagttcatcc agtgcttcaa tatcacgtac gcgccccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacgtcata gccgccgcgc
      421 atgttcagct ttggcgatac ggtaacccag gtattcggtg tgcagcgtac ttcatgagtt
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcaggtcagt cagtggcagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt atatccctgc
      601 cgaccaatga cagccagcaa atcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat
      901 gtcttattaa cattctgtta caggcagatt taaagaaaaa tgcgaaacat atc
//
LOCUS       DAZFJF010000431          950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFJF010000431 DAZFJF010000000
VERSION     DAZFJF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.434"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(149..669)
                     /locus_tag="ACHKAU_005552"
                     /pseudo
     CDS             complement(149..669)
                     /locus_tag="ACHKAU_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            713..793
                     /locus_tag="ACHKAU_005553"
                     /pseudo
     CDS             713..793
                     /locus_tag="ACHKAU_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          279 a          175 c          202 g          294 t
ORIGIN      
        1 aacattgaga taataaaatc actatgtagt tagcttttgt taaaactgca attatttgta
       61 aacataataa taagtattat gacgttaaaa tttaaccata cggtaaaatg attcaggaaa
      121 accggttcag catagcctag gctgaacttt tgaggatatc aacgaacgga atttttgaac
      181 atttatgagt agatcgttgt tgaagggctt caatgagcat gtcaagttca tcaactgctg
      241 gtctacgatt acgacggttt gatttaccaa tcaaagcaag tttaagtaga tatggacgag
      301 cacttttcgc cgggtttgat gtgtaattaa ttccgtatat aggtttggct gcatccagaa
      361 cactgccaag ataactaaca tcgtgactga ctgttgctgg acctgcacca gcgttgtttc
      421 tcagcctgca atgttcaatt acgtcatttt ctgtcagttc agatagtttg atcgcggaga
      481 tgtcactatc cataagcagt tctagcacat atcttttagt acggtctgct ttacctccgg
      541 catttggtca tttaaatatt tgtgtagtaa gtcacggact gtaagtccgt caacagcatt
      601 tgatgatgaa atgccatata gatctaatac catcactttc tgtgtgcccc atgttttagc
      661 atgagcatga acgcggcggc tatctcacgg gtgatcatcc ctttggcata ctgaaaagta
      721 tcgggtgtta ctgaaccgtg ttgatacatc aactgcactg gatattgagt ggccgataat
      781 accgacgtta taacctataa atgccaatca ggctgaatgt tgaaaggata gaatgaacag
      841 cccgacacac aaaaaacgga gtccggctcc ggtttttgtg ttgccatata aggcagatgt
      901 ttgttacagc tatttaagtc tggagttcaa attaaaatag ggagttttgt
//
LOCUS       DAZFJF010000432          935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFJF010000432 DAZFJF010000000
VERSION     DAZFJF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.435"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..224
                     /locus_tag="ACHKAU_005554"
     CDS             <1..224
                     /locus_tag="ACHKAU_005554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534360.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(337..533)
                     /locus_tag="ACHKAU_005555"
                     /pseudo
     CDS             complement(337..533)
                     /locus_tag="ACHKAU_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            709..>935
                     /locus_tag="ACHKAU_005556"
     CDS             709..>935
                     /locus_tag="ACHKAU_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5534361.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          247 a          249 c          234 g          205 t
ORIGIN      
        1 agcagcgcta cgatgactcc ggtgttatct gcaccggagg accgggaaca ttaagcggta
       61 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
      121 ggctgatttt caggtattac cggcctgtat ctgctgaagg gggctcgtat ttgtcggggc
      181 tgcgtattcc gctcgattcg atcacggatt ggcggaatac ctaactgtga ttgattcagg
      241 taccattttg cagggatccg gtgcgttact gtctgacaca acaaacgcag cgggtcgctc
      301 tgctccgccg attcctgata atccctcccc agtaacctac tgcaacagta caaatacact
      361 gacgcctgaa acggtaacca gaatcagcac cagcagtaac tcagtaaaca gagcagacga
      421 tcggtcttcc ggtaataacc ggaaggaacg acagcgccac aatacaggca acacactgta
      481 ttatgagtaa tcgaattacc gcaatccccg catttctttt tgcgtatcac gatagctccc
      541 tcgccacgcc ttatccgtaa ccggtttttt acattaaaaa aaacaacgcc gggaattcaa
      601 tcgtcagtcc ggagacgacc gttcgggtta tcacagagag cctgagcagg ggggctgcgc
      661 attttttacg atgtggctat tccgtatgaa ccatacggag atataaccat gacctacaaa
      721 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
      781 ccccgcctga cggcattgcg ggttgacctg cgtttcccgg atgtaccggc agcaacggac
      841 gcagctgtga tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa
      901 cgtaagcatc gggaaggtaa acgcgtgcat cccac
//
LOCUS       DAZFJF010000433          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFJF010000433 DAZFJF010000000
VERSION     DAZFJF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.436"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..922
                     /locus_tag="ACHKAU_005557"
                     /pseudo
     CDS             1..922
                     /locus_tag="ACHKAU_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    23..139
                     /locus_tag="ACHKAU_005557"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          231 a          219 c          271 g          201 t
ORIGIN      
        1 acgatatcct tcgccaggct tccgcttatt ttgcgaaggc ggagttcgac cgcctctgga
       61 aaaaatgatg ccactgctgg ataagctgcg tgagcagtac ggggtcggac cggtatgcag
      121 cgaactgcat attgccccgt caacgtatta ccattgtcag caacagcgac atcatccgga
      181 taaacgcagt gcccgtgcgc agcacgatga ctggctgaag aaagagatac agcgcgtata
      241 cgatgaaaat caccaggtat acggtgtgcg taaagtctgg cgtcagttgt tacgggaagg
      301 tatcagagtg gccagatgca ctgtggcacg tctcatggca gttatgggac ttgccggtgt
      361 tctccggggt aaaaaggtcc gcactaccgt cagccggaaa gccgttgccg catgcgaccg
      421 cgtaaaccgt cagttcgtgg cagaacgtcc agaccagttg tgggtggctg attttacctg
      481 ggtaagcaca tggcagggct tcgtttatgt ggcgttcatc attgatgtgt tcgccggata
      541 catcgtggga tggcaggtct catcatccat ggaaacaaca ttcgtgctgg atgcactgga
      601 gcaggcgttg tgggcccgtc ggccgtccgg cacaatccat cacagtgata aaggttctca
      661 gtatgtatcg ctggcctaca cgcagcggct taaggaagcc ggattactgg catcaacagg
      721 gagtactggc gactcgtatg acaacgcgat ggctgagagc atcaatggtc tttacaaagc
      781 ggaggtaata caccgtaata gctggaaaaa ccggacagaa gtggaactgg ccacactaac
      841 gtgggtggac tggtataaca atcgacgatt gctggaaagg ctgggccata ttcctccggc
      901 agaagcagaa aaagcttatt at
//
LOCUS       DAZFJF010000434          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFJF010000434 DAZFJF010000000
VERSION     DAZFJF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.437"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..495
                     /locus_tag="ACHKAU_005558"
     CDS             61..495
                     /locus_tag="ACHKAU_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO5534362.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          222 a          204 c          274 g          216 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagtggta agcgtacagc ctgaaccgtc
      781 tggtcagaat ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaaccaaa
      841 gtgtcagata tgcagaaaaa tgtgactccc ggcaggcgaa aaggctgccc taattatcct
      901 cccgaattta aacagc
//
LOCUS       DAZFJF010000435          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFJF010000435 DAZFJF010000000
VERSION     DAZFJF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.438"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..666
                     /gene="tnpA"
                     /locus_tag="ACHKAU_005559"
     CDS             <1..666
                     /gene="tnpA"
                     /locus_tag="ACHKAU_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO5534363.1"
                     /translation="QTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTL
                     FQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSREFKV
                     RLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTP
                     EPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIREMKG
                     GTR"
     gene            663..>899
                     /gene="tnpB"
                     /locus_tag="ACHKAU_005560"
     CDS             663..>899
                     /gene="tnpB"
                     /locus_tag="ACHKAU_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5534364.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWP"
BASE COUNT          227 a          233 c          232 g          207 t
ORIGIN      
        1 cagacaaaaa aagatattcc ctgcttccgt tcttatttgc ctgatgctct gcgtttaaga
       61 tttgaagata aactgaccat ccgggccatc gctcagcgtc taggtctcag tcattccaca
      121 atacatacgc tttttcagcg atttattgca tccggtatcg catggccatt gcccgattca
      181 gtttcattag ctcagcttga tgccatcctt tatgccaaca gaaagaagga attaacagag
      241 cctcaaatca gcgaaggcac atggcgaaaa gaacggcgag ccagctacag ccgtgaattt
      301 aaggtccgtc tggctaagca ggcattacag cccggtgctg ttgttgcccg gatcgccaga
      361 gagcacggta tcaatgataa cctgctgttt aaatggaaaa gccagtacga ggacggctta
      421 ctgagcgatg atgacataca ggaatgcatg cctgtcccgg tggctctgac tgatacgcca
      481 gagccgacca gaccagttac aaatcccttc tggcgtaaca agcctgatga gtgccctgag
      541 agtgatcccg gaaacgtccc acggtgcgag ctgcatctta aatcaggtgt ggtaaaactg
      601 tttgaccctc tcactccgga aatgttacgg gcgctaatcc gcgaaatgaa aggaggtacc
      661 cgatgataac gctgccaacc ggtaccagaa tctggatcat cgctggcatc acagatatgc
      721 gttgtggctt caacggcctg gcttcgaagg tgcagaacac gctgaaagat gacccgttct
      781 ccgggcatat cttcgtcttc cggggccgca gtggcaaaat ggtgaaaata ctgtgggccg
      841 atcgtgacgg gttatgcctg ttcgccaaac gcctggaacg gggccgcttc gtctggccg
//
LOCUS       DAZFJF010000436          896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFJF010000436 DAZFJF010000000
VERSION     DAZFJF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.439"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..189)
                     /gene="kil"
                     /locus_tag="ACHKAU_005561"
     CDS             complement(25..189)
                     /gene="kil"
                     /locus_tag="ACHKAU_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5534365.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            complement(158..322)
                     /locus_tag="ACHKAU_005562"
     CDS             complement(158..322)
                     /locus_tag="ACHKAU_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO5534366.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            complement(395..763)
                     /locus_tag="ACHKAU_005563"
     CDS             complement(395..763)
                     /locus_tag="ACHKAU_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO5534367.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
BASE COUNT          245 a          188 c          185 g          278 t
ORIGIN      
        1 atcgcccgcg aaatgccagg ctgattagga aaacaggaaa ggggggtagt gaatgctttt
       61 gcttgatctc agtttctgta ttaatatcca ttttttataa gcgtcgacgg cctcacgaaa
      121 catcttttca tcgccaataa aagtggcgat agtgaattta gtctggatag ccataattgt
      181 ttgatccatt tttcgggact cctggctgat taagtatgtc gataaggcgt ttccatccgt
      241 cacgtaattt acgggtaatt cgttcaagta aagattcgga agggcagcca gcaacaggcc
      301 accctgcaat ggcatattgc atggtgtgct ccttatttat acataacgaa aaacgcctcg
      361 agtgaagcgt aattggtatg cggtaacgcc gcgctcaggc ggctttgata gtcatatcat
      421 ctggatcaaa tattcctgat gtatcgatat cggtaattct tattccttcg ctaccatcca
      481 ttggaggcca tccttcctga ccatttccat cattccagtc gaactcacac acaacaccat
      541 atgcatttaa gtcgcttgaa attgctataa gcagagcatg ttgcgccagc atgattaata
      601 cagcatttaa tacagagccg tgtttattga gtcggtattc agagtctgac cagaaattat
      661 taatctggtg aagtttttcc tctgtcatta agtcatggtc gatttcaatt tctattgatg
      721 ttttccagtc gtaatcaatg atgtattttt tgatgtttga catctgttca tatcctcaca
      781 gaaaaaaatc gccctcacat tagagggcaa agaagatttc caataatcag aacaagtcgg
      841 ctcctgttta gttacgagcg acattgctcc gtgtattcac tcgttggaat gaatac
//
LOCUS       DAZFJF010000437          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFJF010000437 DAZFJF010000000
VERSION     DAZFJF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.440"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..153)
                     /locus_tag="ACHKAU_005564"
     CDS             complement(<1..153)
                     /locus_tag="ACHKAU_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5534368.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDA"
     gene            complement(143..>895)
                     /locus_tag="ACHKAU_005565"
     CDS             complement(143..>895)
                     /locus_tag="ACHKAU_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5534369.1"
                     /translation="SELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNNVTLGG
                     VKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSA
                     RASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSR
                     AEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQK
                     AGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT          207 a          270 c          217 g          201 t
ORIGIN      
        1 agcatcaaac tgtacctgct gttgtggtac cgaaagactt gatgccccca tctcgcgccc
       61 gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggtt cattgaatgc
      121 tgcggcaata atgtgtgaaa gattacgtct cacgtggtgt tgtctcctct tccggcctgc
      181 gactctccgc tatctgctgc tcatacgcct gcgctatcca caccggacgt gagagtccgg
      241 ctttttgtcg ctcagcagat tccctgacct gctggcggaa aatgtcctga taatcctcgc
      301 ccatcagcgc cagctctttc tcatacgtgc tcagtccggc ctcaatgcgc atcactgatt
      361 cctggacttc cttgagcccg tcaatggcca ttcttccggc tccaatccac tcagcccgtg
      421 accaggctga tcgcgcctga taaaaatcaa aacgcgcccg tggcggacga ataatccccc
      481 gaagaagtgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg gacgcaataa
      541 attttcgtcg tcccataaaa tagcgccacg actcattggc ggaggcgcgg gcacttgaat
      601 aactgacctt cgagtaatcc cgggacaact gttcgtagga aacgccaaga ccggcggcga
      661 tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc tgcgcagtct
      721 gcagtttcag atcatcaccg gggaaaaggt gcggaatttt gacaccgccc agcgtcacgt
      781 tattcgtgtc ataccaggtg gagaacttat ccagaatatt aataagcgga ttatccttct
      841 gcccctgcgg cgcaccggcg atatattcaa aggccttttc ggtatcaagt tcact
//
LOCUS       DAZFJF010000438          894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFJF010000438 DAZFJF010000000
VERSION     DAZFJF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.441"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>894
                     /locus_tag="ACHKAU_005566"
     CDS             <1..>894
                     /locus_tag="ACHKAU_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5534370.1"
                     /translation="YKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISDSFGGPL
                     SLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAAGGGVAV
                     LAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIRQAAENL
                     ARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYN
                     FLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTW
                     TSDAWAWIGDHTLGALSLITDV"
BASE COUNT          200 a          219 c          296 g          179 t
ORIGIN      
        1 gtataaggct gccatgcgca cactgcccgc acagttcacg gatattgtca ctcagcttgc
       61 cggtggtcag aatcccttcc ttatcatgct gcagcagggg gggcagatca gcgattcatt
      121 cggtggaccg ctcagcctgc ttaccctgct gaaggaggaa cttctcggga tcagggatgc
      181 ctctgaatca tcagaggagt cgctgtcaga tacggcaaat gcactggctg aaaatgcccg
      241 gaatgccggt gagctgggac gatttatgtc ggtggcccgt gtggcggcag gtggcggggt
      301 tgccgtactg gccgcgcttg ctgccgccgc ctggcaggca gagcaggctg accgggcctt
      361 attgcgttca ctgatcctga ccggaggggc ggctgccacc acaacggcag aattgtggaa
      421 aatggccggg gtgatcagcg atgaagccgg tggtggtatc agacaggcgg cagaaaatct
      481 ggcccgtctg gcagaaagcg ggaaatatac cgccgggcag ctacggatca tgggggaaac
      541 ctctcagaga tggctgcaga cggtggggga cgatgccggg aaggtggaaa aagcctttga
      601 agggattgca gcagatccgg tgaaggcgct ggcctccctg aatcagcagt ataacttcct
      661 gagcgtttcc cagttacgcc atattgatga gcttgagcgc acgaaaggta aacaggctgc
      721 ggtgacggag gcgatgtccc tgtttgcgga tgtcatgaat gcacgtctgg agcaacttga
      781 taaagcggcc acgccggtgg aaaaaatctg ggacgatgtt aaaacctgga cttctgacgc
      841 atgggcatgg ataggtgatc atacactggg ggcactcagt ctgatcactg acgt
//
LOCUS       DAZFJF010000439          892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFJF010000439 DAZFJF010000000
VERSION     DAZFJF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.442"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..150)
                     /locus_tag="ACHKAU_005567"
     CDS             complement(<1..150)
                     /locus_tag="ACHKAU_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534371.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESL"
     gene            344..769
                     /gene="yqeK"
                     /locus_tag="ACHKAU_005568"
     CDS             344..769
                     /gene="yqeK"
                     /locus_tag="ACHKAU_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO5534372.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            805..885
                     /locus_tag="ACHKAU_005569"
     CDS             805..885
                     /locus_tag="ACHKAU_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534373.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
BASE COUNT          286 a          128 c          164 g          314 t
ORIGIN      
        1 aagagattct tgttcaatat aatttaaacc agaccccatt gtgattttaa ctggattaac
       61 ggatttctct tcatttttct tatttttgtt ctcctgatca ggaactaatg gttgcagtgg
      121 taggctatta ttaatatttc gtggcttcat tttacatttc tctcatttga ttagagcaaa
      181 atccgaattc gcagcttctg ataatgtcaa atatcacctt atggagacat tacgtagaat
      241 gactatattt gttatcggca cattaggggt attaatttta gtattgatgg aaggtgatta
      301 ttgaacagta acatcgagtc acggtaaaaa tgtgaggtat ataatggaca ttgagttttc
      361 gcagattcat gaaatggtct atatgcatga tattgtaaat tcggactcga aaaagaaacc
      421 aaggattccg ttaaaaaaat tccttaatgc tgagaaggtt ctcacgcaaa caacatcatg
      481 ggcattaaat tcaagatttg tgaatgttaa ttctgtcaat aaagtcaacg taaagagtaa
      541 agttaaaagc agttatatct cacgatctgt caatgatgag tttagtttaa cagatgatga
      601 aatcaattct tttaaagaaa cgttagtcct ttcctctatt gattcactga gtaaactggt
      661 tcttaataat cctctttctg tattgtttac atcgactgta cgaaggaata ataatcgagc
      721 taagatgaat gtcgagttcg actcatggat ttgcaccaga tgttgttgat acatgatatt
      781 ttacattgca aggagatgtc taaaatgaaa gatgttgatc aaaactttga tgctttagac
      841 tgccatatac tgcgagaata tttaatttta ttgttttatg attaacgagg tt
//
LOCUS       DAZFJF010000440          890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFJF010000440 DAZFJF010000000
VERSION     DAZFJF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.443"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..285)
                     /locus_tag="ACHKAU_005570"
     CDS             complement(<1..285)
                     /locus_tag="ACHKAU_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534374.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWY"
BASE COUNT          264 a          192 c          185 g          249 t
ORIGIN      
        1 ataccatgct tccccatata tataatctcg gatagcagtc agcaacgtct tatcactttt
       61 gtatccactt tcataacgag gaatattatc cgcaggttga tttagataat atatccacac
      121 agactgcgca cattttgttg ctgtagcagt ttgacgattg ttagtccaaa ggaaaagata
      181 taagcaattc cacaatgcca tgcgtgtatc agaattaaga tcattcagct ggacatgctc
      241 tctaacgtca acatgaccat acctcacaga aaatggcttt atcattcttc ccctgcctga
      301 ttgttatgtt cgtttaaatt atgcagtcta agtttgatac aatcatatat taagcaatag
      361 aatccataaa gccccgttgg tggtaagaca acaagcggag cctcttgcga cttcatcagg
      421 gttatgtcag ttagtgaggc ttatcaggat gaccatgtac ccaaggggag cgaggccgaa
      481 ccgctggcag agcttgcata tggtctgcaa gtaccgtgac caccataaac ctgtttatcc
      541 cctgcggcgt ctcgcgacgg cgacagaatg ggggcatcgg gctaaaatgc gctgcactaa
      601 aagttttggg attgtttaga gacggtggct acgtcatagc tttcaagttg agtcagtacg
      661 tgtttgacgc ttggaacgac acgccgctat gagttaacat cccaattacc actatcaacc
      721 acagcggctt tttcaaggga cataacagag catttcttac aggcaaaaga gacagattta
      781 atcgtctaac taactgtatt gtaaggaatt tagtaacttg gtgcgaaggc cggactcgca
      841 cacacattta acctattgta aaaattgaaa tttaattata attaattcaa
//
LOCUS       DAZFJF010000441          887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFJF010000441 DAZFJF010000000
VERSION     DAZFJF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.444"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..222
                     /locus_tag="ACHKAU_005571"
                     /pseudo
     CDS             1..222
                     /locus_tag="ACHKAU_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            261..>887
                     /locus_tag="ACHKAU_005572"
     CDS             261..>887
                     /locus_tag="ACHKAU_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO5534375.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDI"
BASE COUNT          316 a          179 c          143 g          249 t
ORIGIN      
        1 gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta tggcaaggtt
       61 accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac ttatgcccgt
      121 ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg tcactgggaa
      181 tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaagaa tctctgattg
      241 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
      301 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
      361 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
      421 tatcatcaaa acatctttgg catgtattag ttaacgtcat caacaatgat ccacttgcac
      481 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
      541 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acctctatca
      601 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
      661 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
      721 ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat aggctttcat
      781 cagttactta ttttagtgcg gcacataatc aactagagtt tgtgcaatta gaatcttgcg
      841 aatggctgca atacctgaat ctcagccata atcaattaac tgatatt
//
LOCUS       DAZFJF010000442          872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFJF010000442 DAZFJF010000000
VERSION     DAZFJF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.445"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..560)
                     /locus_tag="ACHKAU_005573"
                     /pseudo
     CDS             complement(1..560)
                     /locus_tag="ACHKAU_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            complement(673..>872)
                     /locus_tag="ACHKAU_005574"
     CDS             complement(673..>872)
                     /locus_tag="ACHKAU_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5534376.1"
                     /translation="LLTAQLRLGPADILESDENGIIPEQDRVITQVVILDADKKQIQC
                     VVRPLQILRADGTWENIGGMK"
BASE COUNT          241 a          225 c          162 g          244 t
ORIGIN      
        1 aataaagctt ccccttgcag ggtcatattt acactcttct ggtttaacaa tcattgatgg
       61 ggttattgga gctcgtgtca gagggtgaac tgaaccgtca ttaaccacgc gagaaagtgc
      121 atcaacatca aataaagtgc atactgctga actatctgaa tttttgacaa acactccttc
      181 ttcgggtctc tctagtgtaa ttggacactg aatagactct ggcgggcact ggaaatttcc
      241 tgaactaaca ggaaacttac attgagatat tttgtcctga atactctctt gcgattgtgc
      301 ttcacctgag tccgaaagca tactgagcat cctatcgcga agagcttcag ggccgttatt
      361 aaacccataa aggaataact cagtaagcaa tcccccacta ctgccgctgg atagaaaacg
      421 cccatcggtt tcattaaata caatgctgac agtctcatta cctaatgtaa actcccctcg
      481 accgctagat gctacttgag ctcgaaggac attcaatcca ttaaacatga gcttgatcta
      541 atctccgaat ttaacggcat tataaaactc cctattttaa tttaaactcc agacttaaat
      601 agctttaaca aacatctgcc ttacatgaca acaaaaaccg gagccggact ccggttttgt
      661 gaagctgtcg ggttacttca tcccgccaat attttcccac gtcccgtcag cacgcaggat
      721 ttgcagcggt cttaccacac actgtatctg ctttttatcc gcatccagta tcaccacctg
      781 cgtgattacc ctgtcctgct ccgggataat gccattctca tctgactcca gaatgtctgc
      841 cggtcccagt cgcagctgtg ctgtaagtaa ct
//
LOCUS       DAZFJF010000443          869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFJF010000443 DAZFJF010000000
VERSION     DAZFJF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.446"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..184)
                     /locus_tag="ACHKAU_005575"
     CDS             complement(<1..184)
                     /locus_tag="ACHKAU_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5534377.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDII"
     gene            complement(184..399)
                     /locus_tag="ACHKAU_005576"
     CDS             complement(184..399)
                     /locus_tag="ACHKAU_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5534378.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            542..>869
                     /locus_tag="ACHKAU_005577"
     CDS             542..>869
                     /locus_tag="ACHKAU_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534379.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNN"
BASE COUNT          244 a          232 c          170 g          223 t
ORIGIN      
        1 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
       61 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      121 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      181 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      241 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      301 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      361 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      421 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      481 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
      541 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
      601 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
      661 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
      721 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
      781 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
      841 cacaaggaaa atgatagaag gaaataaca
//
LOCUS       DAZFJF010000444          866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFJF010000444 DAZFJF010000000
VERSION     DAZFJF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.447"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>866
                     /locus_tag="ACHKAU_005578"
     CDS             <1..>866
                     /locus_tag="ACHKAU_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO5534380.1"
                     /translation="KLNVEKKPDINDIINVAILETSYLQIKKIPNNDDGSYVLCLDNP
                     NLSSYTLHFNDLSGYISSIWDNIRGSFTPFHNNTVNIAPNEKKFISLTEPDKLSFNID
                     VFREALEVKNSYKLSKFTWETYGDIVVSPEDRISHLELDGFNYFSQPELDAQISDTFS
                     YLYDNFQIADGDVHIKLLHLDRETKQITPHRIILQKYFIDSFAKTSISDRENNIYPVI
                     CDSPDHFTNDIYRHPFRIVLGNKTLYPSEELIKFIRTSKEYLSNMDIINNIIVPQKTI
                     KKNKLSVVSLNS"
BASE COUNT          329 a          134 c          123 g          280 t
ORIGIN      
        1 taaattaaat gtagaaaaga aaccggacat taacgacatc atcaatgtgg ctattttaga
       61 aacttcttat ctgcaaataa aaaaaatccc aaataatgat gatggcagtt atgttttatg
      121 tctagacaac cctaacctct catcttacac attacacttt aatgacttat ctggatatat
      181 atcaagtata tgggataata ttagaggaag cttcactcct tttcataata atactgtgaa
      241 tatcgcccct aatgaaaaaa aatttatttc cctaacagag ccagataagt tgtcattcaa
      301 cattgatgtt tttagagaag cattagaggt aaaaaatagt tataagttaa gtaaatttac
      361 atgggaaacg tatggtgata tcgtggtatc acctgaagat agaatttctc atctggaatt
      421 agatggattt aattattttt cacaacccga gttagatgct caaatatcag acaccttttc
      481 atatctatat gataattttc agattgctga tggtgatgtt catattaagc ttcttcatct
      541 cgatagagaa acaaagcaaa taacgccaca tcgtattatc cttcaaaaat actttataga
      601 ctcatttgct aaaacaagta tctctgacag agaaaacaat atatatcctg ttatttgtga
      661 ttcacctgat cacttcacaa atgatatata taggcatcca tttaggatag ttctcgggaa
      721 taaaacattg tatccatcag aagaacttat aaagtttata agaacctcaa aggaatattt
      781 aagcaatatg gatataatta ataatattat tgttccacaa aaaacaataa agaaaaataa
      841 attatctgtc gttagtttaa atagca
//
LOCUS       DAZFJF010000445          861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFJF010000445 DAZFJF010000000
VERSION     DAZFJF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.448"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..747
                     /locus_tag="ACHKAU_005579"
     CDS             <1..747
                     /locus_tag="ACHKAU_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO5534381.1"
                     /translation="FDYGYKKSPQEYSLSFIKQLPNTVNLSSLTYNKEDDFLYATQNS
                     PAQLLKIARSGDIIGRAPLPFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHIS
                     LRNKIQLEKFNKKNRGFEGLAWKAEGRMLFVAKERRPTGMFAYQLSPDLLSAKQVTIP
                     EELNDIHVKDISGLDFNNESLMILSDESRKLLKFNLTEMSFVEMMDLTKGNHSLTSDL
                     LQPEGIVTLSDESIYVASEPDILAKFVPNK"
BASE COUNT          290 a          172 c          158 g          241 t
ORIGIN      
        1 tttgattatg gttacaaaaa atcaccacag gaatactcac tttcatttat aaaacaatta
       61 ccaaatacgg tgaacttatc atctcttacg tataataaag aggatgattt cctgtatgcc
      121 acccagaata gccctgcgca gctacttaaa atagccagaa gtggcgacat catcggccga
      181 gccccgctgc cttttatctc tgatgccgaa accattgagc acatacaagg gaacattttt
      241 gctgcagtag acgaaaagac ttctgaacta ttctttttta cagttacaaa agatatgcat
      301 atctccttgc gaaataaaat tcagttagaa aaattcaata aaaaaaatag agggtttgaa
      361 ggactggcat ggaaagcaga aggccggatg ctgtttgtcg ctaaagaacg acgccctaca
      421 ggcatgtttg cctatcagct ttctccagat ctgctgagtg caaaacaagt aacgattcca
      481 gaggaactaa atgatataca tgttaaagat atatccggcc tggactttaa taatgaaagt
      541 ctgatgattc tgtccgatga atcgcgtaag ttactcaagt ttaacctgac agaaatgtct
      601 ttcgttgaga tgatggacct gacaaaagga aatcattctc tgaccagtga tttgctccaa
      661 cctgaaggca ttgtaactct gtctgatgaa agcatttatg tcgctagtga acctgatatc
      721 ctcgctaaat ttgtccctaa taaataagtc tgtcactagc cttagtattg agcgaccaaa
      781 tctggcaatc tcatgaaact cgcaaaatac gaatctaata ttaataaaaa ttttactctg
      841 aaataaacag attaatagct g
//
LOCUS       DAZFJF010000446          853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFJF010000446 DAZFJF010000000
VERSION     DAZFJF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.449"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(393..>853)
                     /locus_tag="ACHKAU_005580"
     CDS             complement(393..>853)
                     /locus_tag="ACHKAU_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5534382.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          207 a          225 c          227 g          194 t
ORIGIN      
        1 ggtgttcaga tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac
       61 ccacaaatgt ggataccggc aggtacaaaa cagcatgctc agataaagtg gtttttctcg
      121 gacataaatg cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc
      181 cgttctgcat gatgaagaga ttcagctgcc ttttcgcaga ccattaacca ctataaacaa
      241 tcgatagcaa cgatcgatac ctgttttatc gatcgtttta ttctattaac gacaaggcgg
      301 tcacgcaaga gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg
      361 taatgttatc aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagcg
      421 cgggcaacag agaccaccgt gtcacggacg gcacgcagaa ccgtacgggc aacagaggca
      481 acacagacgc tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg
      541 gcctgagcgc ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg
      601 gcggcatggt cgggaagtgc tgtcattagc gcactgacca gcgtatccag ttcccagccg
      661 gtgcgggccg atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc
      721 gttttctccc gaatgttctg tgcctgtgcg ggagaaggct gaatgccggc catatcccat
      781 tcatggcagg gctccgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc
      841 cggtgcagga tgt
//
LOCUS       DAZFJF010000447          851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFJF010000447 DAZFJF010000000
VERSION     DAZFJF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.450"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            69..479
                     /locus_tag="ACHKAU_005581"
     CDS             69..479
                     /locus_tag="ACHKAU_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534383.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEFNALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPDFQILDNADHYGDF"
     gene            481..816
                     /locus_tag="ACHKAU_005582"
     CDS             481..816
                     /locus_tag="ACHKAU_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534384.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPASLNEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIEDIESDVRYVGDGLVELA
                     QRLGTITEE"
BASE COUNT          301 a          167 c          160 g          223 t
ORIGIN      
        1 attgttcttt ctcacgttag aaacatactt cggggtgttt tcctttaaaa gaaaaggaaa
       61 agtaaataat ggcaaaaaga aaaaacaacg ttgttaagaa aatcggggat tcagcttctc
      121 ttctaagtaa acctaaatct attctaagac gcaaagactt taaagagcta gttacgctca
      181 ccaagcaaaa tagcgcccct ggcgaatgga aaacagagat catagaacat tctccctctg
      241 tgccttgcgg agatgaattt aacgccttac aagaaatctt atcttcaaca cctggcgtat
      301 tctggaaacc tagaaaaaga aaagagtata tcgttgatag ctctgattta cgcaaatacc
      361 agattttagg atttgaagac tataaccact acgttggtta tctcgccaca aatggcctaa
      421 ataacttagt tccagacttc cagattctag ataatgctga tcactatgga gacttttaat
      481 atgaacaaaa acacgtatga cacaatttac tcactaatta attattatga ggatgattac
      541 cttctgcctt taaaccgcgc tgaacttgaa gcatacaaag aaaacacacc agcgtcacta
      601 aatgaggcgt ttaaacactg ggatttagct gtgaatgcct ttgagcacct ctctaagcgt
      661 gtagagatgc tctgtaagcg cgaaaacgca tacctgacag ccgatcagat atgggagcta
      721 tcaaactgga tagaagatat cgaatctgat gttcgctatg tgggagatgg acttgtcgaa
      781 ctcgctcaac gcttaggcac tatcacagaa gaataaaaaa agcaacaaaa aagttgtaaa
      841 atatcttgac a
//
LOCUS       DAZFJF010000448          846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFJF010000448 DAZFJF010000000
VERSION     DAZFJF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.451"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..648)
                     /locus_tag="ACHKAU_005583"
     CDS             complement(<1..648)
                     /locus_tag="ACHKAU_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5534385.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAEIEMPDFVREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQ"
     gene            complement(654..>846)
                     /locus_tag="ACHKAU_005584"
     CDS             complement(654..>846)
                     /locus_tag="ACHKAU_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5534386.1"
                     /translation="ANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCK
                     FHNNVGNFGGFLSINKLSQ"
BASE COUNT          189 a          274 c          211 g          172 t
ORIGIN      
        1 ctgccatttg tcggtatacc tctcccgttt actcagttgt tcaggaatat ggtgcagcag
       61 ctcgccgtca ccacagtaaa tagcggcgtg attcggcacc gatgaaccaa aacagcacag
      121 cagcacatcg cccggctgcg ccgctgacaa cggcacctga tacagccctg tggcctccag
      181 attatccaga tagagattct gaccgttacg ccaccagtca tcctcacgca caaaatccgg
      241 catctcaatc tccgccagat gataagcatc ccggaacagt gtgtaacagt ccgtcacacc
      301 gtgctcaaag cgccgcccgg tgagatgcgg cacacagcgg aacttatgaa tctcaccccg
      361 gcagaccagc caccacggca aatcactctg cacctgcagc cgccggtcgg cctcactcag
      421 ccagggcaga ccaccggggt ggctgtggac cagcgccaca atctccccct gcatttctgc
      481 ctgcagccag tcttccggcg acatacggaa atacgcctcc ggctcaccgg agatattcac
      541 gcaggggaaa tatctttccc cctccggcgt gcttaccacg aagccgcacg actccgctgg
      601 cgcacatcgc cgggcgtgcg ccagaatcgc tgattctgtc tgtgtcatgg gatttactgc
      661 gaaagtttgt taatggaaag gaagccgcca aagttgccga cgttattgtg gaacttacaa
      721 ccgctcaggc atttgctgca tttatccttc gtgatatcgg acgttggctg gtcatattca
      781 tccgcgacag ccggaccgct ataaccgcac tcgtcaccgc gataggtcca ggtgcaggtg
      841 ttggcc
//
LOCUS       DAZFJF010000449          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFJF010000449 DAZFJF010000000
VERSION     DAZFJF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.452"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..303
                     /locus_tag="ACHKAU_005585"
     CDS             <1..303
                     /locus_tag="ACHKAU_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534387.1"
                     /translation="PPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNGVEIFHEPATD
                     VSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKKSTAGISIS"
     gene            372..>845
                     /locus_tag="ACHKAU_005586"
     CDS             372..>845
                     /locus_tag="ACHKAU_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5534388.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDG"
BASE COUNT          196 a          197 c          253 g          199 t
ORIGIN      
        1 cccccggtgc tgtttcgcgg gacgaaacac cagaatttca acagcccgaa tcagcagtcg
       61 tactggtatt ccacctgtaa gctgcaggtg ctgaagaacg gggttgagat tttccatgaa
      121 ccggcaacgg atgtcagccg ggtgttctca tcggtgatag atatgccggc agggcggggt
      181 catgtcaccc tgacgtttaa tgtgtcgtcg gccggtgcga acaactggac gccgacaacg
      241 tacatcagtg atttactggt tgtggtcatg aaaaaatcca cggcagggat cagtatcagc
      301 tgacggttta ttaacccgga cgggcacctc aggaggtgcc ttttttattg actgaaaaca
      361 aagaggtaat catgcggcat ttatacgcaa cgatattatt gtttactacc ctgctggcag
      421 gaattgcctt tcctgcacag gctgaaagcg gacacggtgc attttccgtg ggatatgctc
      481 aggttcaccc gggcggcgta ccggcattgt ccggtaccgg tgcgcgtgca ggtgatttaa
      541 aagggattaa tgtgaaatac cgttatgagt tcacggatca cctgggcggc attgtcgcgc
      601 tgagttatgc atcggtgaag aaaagtgaca cgatgaagac gggtgaaaat accttccatt
      661 atgaaagcct gcgcggtcgt tatgtcagtc tgatggccgg ccctgtctgg cagctcagtg
      721 agcgggtcag tctctatggc atggccggga tggcgtacac ccgctggtct gacagtgttc
      781 aggattaccg gcgtgatgaa gtgaaaccgg ggtatgtgaa ggagaccacc accgccagtg
      841 atggc
//
LOCUS       DAZFJF010000450          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFJF010000450 DAZFJF010000000
VERSION     DAZFJF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.453"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..746
                     /locus_tag="ACHKAU_005587"
     CDS             <1..746
                     /locus_tag="ACHKAU_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO5534389.1"
                     /translation="VSVINPALGKAFAQSEGLRNKTDTVDARMLAEFCRQKRPAAWEA
                     PHPLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLLWLEAELKRLEKQI
                     KDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRY
                     ESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGA
                     MMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          204 a          205 c          267 g          158 t
ORIGIN      
        1 tagtgtcagt cattaatcct gcgctgggta aagctttcgc tcagagtgaa ggactgcgta
       61 acaagactga taccgtggat gcgcgcatgc tggcagagtt ctgtcgtcag aagcgccctg
      121 cagcctggga agcgcctcac ccgcttgaac gcgcgttgcg tgccctggta gtccgccacc
      181 aggcgctgac agatatgcac acgcaggaac tgaatcgcac tgaaacggcg cgggaagtcc
      241 agagaccgag cattgatgct caccttctgt ggcttgaagc agagctgaag cgtcttgaga
      301 agcagataaa agacctgaca gacgatgatc cggatatgaa acaccgcagg aaactgctgg
      361 aaagcatccc gggtatcgga gagaaaacat ctgcggtatt gctggcttat atcggtctga
      421 aggaccgctt cgcccatgcc agacagttcg ccgcttttgc gggtctgaca ccacggcgtt
      481 atgaatcagg tagcagtgtg agaggggcga gccggatgag taaggccgga catgtgtcgc
      541 ttcgcagggc gttgtatatg cccgcaatgg tagccaccag taagactgag tggggacggg
      601 cgttccgcga ccgtctggcg gctaatggca agaaaggaaa ggtgattctc ggcgcgatga
      661 tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcgtgccg ttcgatgcgt
      721 cacggcataa tccggtagcg gcgtaaaaat cgcggaaggg atgaaaaaaa cagcgcctga
      781 cggcgctgtg tctggcatgc ctgcaatccg ggaaaccgga ccaggaaaaa actt
//
LOCUS       DAZFJF010000451          823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFJF010000451 DAZFJF010000000
VERSION     DAZFJF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.454"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..510)
                     /locus_tag="ACHKAU_005588"
     CDS             complement(<1..510)
                     /locus_tag="ACHKAU_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5534390.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
     gene            487..629
                     /locus_tag="ACHKAU_005589"
                     /pseudo
     CDS             487..629
                     /locus_tag="ACHKAU_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            675..>823
                     /locus_tag="ACHKAU_005590"
     CDS             675..>823
                     /locus_tag="ACHKAU_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534391.1"
                     /translation="MAKHGAGCLPVNSVSAPESAIIPYTWLLIAPPHRGIHHAEVFLI
                     NSKQK"
BASE COUNT          201 a          231 c          204 g          187 t
ORIGIN      
        1 ttttttggcg atatgcagcc cttgccccac ggttccgtac tgcccctttg acaggtccgc
       61 tttcggatgg ttaaggcggc tcatgtcctg cacatcatgc agacaatggt ccgccggaat
      121 gatgtcgtta tatttacagg cgacaccgcc cggtgtcacc gtactgcgac gcgccagctg
      181 cttaatgcgc gggtccggac ggtcatatgt ctccggcagc ggaaggcctt caccatatgc
      241 catgctgttt gactgccccg ccagaaccac aacaaagtaa tactccgggt ctctggtggc
      301 gctgattact gtgccttctc catccgacgg cttcaccaac acaggtgtgg tgacatcacc
      361 ttccgccgca atggcctgca tcagagtata aggcgtgatg gccaccgggc tgccaaatgg
      421 ctgccacccc tccttcagtt tttgtgtcag cttttccgca aggtctgacg gcgacgccgc
      481 cctgacaaca tcataatgtt taatcgacat cgaatttctc ccgtgtacag gaacagagtt
      541 aaaaagccgg aaccggaatc aaattacagg atggccatct gccagtggct ggtcgtaaaa
      601 aaaggccacg ccatgcgcag ccggaaataa agggataacg atgatagttt gagaaaaaca
      661 gaaacaacac ttttgtggca aagcatggtg ccgggtgcct cccggtgaat tcagtatcag
      721 cacctgaatc cgcgattatc ccatatacct ggttgctgat cgcccctccg cacaggggga
      781 ttcaccatgc agaagtgttt ttaataaaca gcaaacaaaa aaa
//
LOCUS       DAZFJF010000452          793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFJF010000452 DAZFJF010000000
VERSION     DAZFJF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.455"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..321
                     /locus_tag="ACHKAU_005591"
     CDS             <1..321
                     /locus_tag="ACHKAU_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5534392.1"
                     /translation="REFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAGMIKQAWC
                     SALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKVHCTGERNFG
                     CSRG"
     gene            487..>793
                     /locus_tag="ACHKAU_005592"
     CDS             487..>793
                     /locus_tag="ACHKAU_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5534393.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     "
BASE COUNT          177 a          217 c          227 g          172 t
ORIGIN      
        1 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg
       61 gatacctggt gtcgtgccgg tgattaccgc gctccgggat cactggccgg gatgattaaa
      121 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
      181 ccggcctggc cggcggtgtg gctggcgcgt aatgatgaca ccggctttca gcaagtgctg
      241 gaacgtgctg actatctggc gaaggagcat accaaagttc actgcaccgg tgagcgcaac
      301 tttggctgca gtcgtggctg agccagacag acgagttatc tgacttcagt gtgatgacgt
      361 gttcacggcc accacactga ttttccctta tgagactgaa ctgaaaccgc cattcacacc
      421 ggaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctgacg gtaaggaaca
      481 tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct gacgggcgcc
      541 cgtacagcac aggccgcacc ttatgctcac tacacctgcc acttgtgtgg cagtgcactc
      601 agataccatc cgcaatacga cactgaactt ccctggtttg aacacactga cgacaggctg
      661 acagagcacg gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt
      721 aaacgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc cagctggcac
      781 tgcagacaat gtc
//
LOCUS       DAZFJF010000453          758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFJF010000453 DAZFJF010000000
VERSION     DAZFJF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.456"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..441
                     /locus_tag="ACHKAU_005593"
     CDS             <1..441
                     /locus_tag="ACHKAU_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFO5534394.1"
                     /translation="ALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFKRHIRHRE
                     ADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYDLRYGHPI
                     NADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            419..>758
                     /locus_tag="ACHKAU_005594"
     CDS             419..>758
                     /locus_tag="ACHKAU_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534395.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVKLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRVT"
BASE COUNT          221 a          155 c          211 g          171 t
ORIGIN      
        1 gcgcttcgtg aggcagcaca aaactatcag tcgacgctgg cgtggtatca ggctaccccg
       61 gacagcccaa atgctgaacg ggattgtgat gcggctcttg ctgcgtttaa gcgtcatatc
      121 cgtcatcggg aagcggatat tatcgctggt ttgctggatg gactggaaga agcaaaatca
      181 caactcaacg agcagcgtga gtattacgaa ggcgttatct ctgatgggag caagcgtatt
      241 gctgaactgg atgcgcggga agttcaatta ccgactcgct acgaccttcg atatggacac
      301 ccgataaatg cagatgagcg acatgtcatg atacctaaag aaaatggcag ttggctttac
      361 ctgattgacc tagaacacgc attacgcgtc gctggcattc gcatcaaagg agaggagcat
      421 ggaaataaaa ccagaggatg agttaagcaa tatcgtttta tttccggtaa aagaggatga
      481 ccctcgtaat caggttaatt ttctttatga gccatcggaa agaccatatt gtcatcacgc
      541 ctctgtccgg gttgacgaaa aagagcgtca ggtccgctgt aaaatctgcg gtgcagttgt
      601 ggagccattt gactggatgc tctctgtggc gaaaagagaa actaggctgg cagatgatgt
      661 aaagctattg cgccaggagg aacaggaaag gcggaaaaat atagaaaagt taattcagat
      721 tgagcgtaac gcgaaagcgc ggatacgcag ggtgacaa
//
LOCUS       DAZFJF010000454          749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFJF010000454 DAZFJF010000000
VERSION     DAZFJF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.457"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     repeat_region   47..624
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=112..136
                     /rpt_unit_seq="tccccgcgccagcggggataaaccg"
BASE COUNT          170 a          219 c          197 g          163 t
ORIGIN      
        1 gtgactggct taaaaaatca ttaattaata ataggttatg tttagagtgt tccccgcgcc
       61 agcggggata aaccgatctc ctcatcctcc cagctaccga cagtagtgtg ttccccgcgc
      121 cagcggggat aaaccgcaag atctgccgcg agtggtttca cccggcaggt gttccccgcg
      181 ccagcgggga taaaccggat atttgggcgt aataccgccc ggatagtgtg tgttccccgc
      241 gccagcgggg ataaaccgtg gtacggaaca tgctgtctat atttcgacca gtgttccccg
      301 cgccagcggg gataaaccgc cagtggagcc gccgcgaatt tacacacgat tgtgttcccc
      361 gcgccagcgt ggataaaccg taaggccgtc gccggatcag cctggctatg ccgagctccc
      421 cgcgccagcg gggataaacc gttcttgcgg gtgttgcaaa tattcttcac gtagagctcc
      481 ccgcgccagc ggggataaac cggagcctga cgagactact gaggccgttc tgtcgagttc
      541 cccgcgccag cggggataaa ccggacgccg ccgccgcgaa gccgtttccg atgttgagct
      601 ccccgcgcca gcggggataa accaccatat aacccgttat ctctttctca agtttttata
      661 ttagcagtac ttgtaataag caacatatcc acgtaacacc tcatgttcaa aatagttctc
      721 catgccagag aggttcacaa ttatcgata
//
LOCUS       DAZFJF010000455          742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFJF010000455 DAZFJF010000000
VERSION     DAZFJF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.458"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..350)
                     /locus_tag="ACHKAU_005595"
     CDS             complement(<1..350)
                     /locus_tag="ACHKAU_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5534396.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDV"
     gene            complement(377..>742)
                     /locus_tag="ACHKAU_005596"
     CDS             complement(377..>742)
                     /locus_tag="ACHKAU_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5534397.1"
                     /translation="LSALQRFDYIKFVSDAEQQETTKHDVVHINQRYLETASLLVAMS
                     LWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATNRVLYLSGMLDNRHDADPEQ
                     TGKAEATEPVTSKKHSKAS"
BASE COUNT          164 a          224 c          177 g          177 t
ORIGIN      
        1 acatcatcct cgctgacatc caccacatcc gcagaaggtg aaatatcgcg ccctccgtcc
       61 cctccgaacc ggacgcctcc ggcaactcct gtcgctttct gcatcagcat ttcttcctcg
      121 tccggcatct ccgtctgctc ttcctcacac gctggagcaa gcagactgaa atccgccgga
      181 tgcatatccg gatcgccaaa aaacaggctg agtacggcgt acatcagccc ggaaaaatga
      241 gcgtccagtt gggtatcctg aaaataatgc gtgcagtaaa aacgtcgcca gtcggcatat
      301 tcggtggatg tcatcccggc aagcatggcg cgccagtcgg gtctccccat ctctcgcgcc
      361 agtttcagga caaagttcag ctcgccttcg aatgcttttt tgatgttacc ggctcagtcg
      421 cttctgcttt cccggtttgt tcaggatcgg catcgtgccg gttatccagc atacctgaaa
      481 gataaagcac ccggttcgtt gcctgattca gtgcatcagc aggccatccc agcatcactt
      541 cacggcggat ctgctgcatc tctgtctccg gagaggccag agtgcctttg agggaatggg
      601 aatgccatag cgacatcgcc acaagcaggg atgccgtttc cagatatcgc tggttaatgt
      661 gcacgacatc atgcttcgtt gtctcctgtt gttctgcgtc tgaaacaaac tttatataat
      721 caaaccgctg cagcgcagac aa
//
LOCUS       DAZFJF010000456          741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFJF010000456 DAZFJF010000000
VERSION     DAZFJF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.459"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            343..558
                     /gene="essD"
                     /locus_tag="ACHKAU_005597"
     CDS             343..558
                     /gene="essD"
                     /locus_tag="ACHKAU_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO5534398.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            558..>741
                     /gene="rrrD"
                     /locus_tag="ACHKAU_005598"
     CDS             558..>741
                     /gene="rrrD"
                     /locus_tag="ACHKAU_005598"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5534399.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIM"
BASE COUNT          187 a          143 c          178 g          233 t
ORIGIN      
        1 tcaatttaaa tcggctaaaa ataacgctgg aaattatttg ttggttattt gttgagattt
       61 gtttatgtat ttgtagtggt gttttcaata ctcggtagca ttctcgcaaa tatcatttag
      121 tggtttacgt acgtaaaaaa ttggttatgc tgttaagagt ggttacttcg tcacacagct
      181 taaacccgcc gtcgagcggg tttttccatt ttttgagtct cgatattagc tgataaccca
      241 atacctgagt tattcactga ctccgagtct gttacgtttc tgcttttttg cgatacgttg
      301 tattccctca atttacaccc gctttgtctg cgaggtgggg ttatgaaatc catggataag
      361 ttaacaacgg gtgtcgccta tggcacctca gcaggtagtg ccggttactg gtttttacag
      421 ctgctcgata aagtcacgcc ctcacagtgg gcagcaatag gtgtgctggg tagcctggta
      481 tttggcctgc tgacgtacct gacaaacctt tatttcaaga ttaaagaaga taagcgcaag
      541 gctgcgagag gtgaataatg cctccatcat tacgaaaagc cgttgctgct gctattggtg
      601 gcggagcaat tgctatagca tcagtgttaa tcactggccc aagtggtaac gatggtctgg
      661 aaggtgtcag ctacatacca tacaaagata ttgttggtgt atggactgta tgtcacgggc
      721 atacaggaaa agacatcatg c
//
LOCUS       DAZFJF010000457          739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFJF010000457 DAZFJF010000000
VERSION     DAZFJF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.460"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..104)
                     /gene="ariR"
                     /locus_tag="ACHKAU_005599"
                     /gene_synonym="ymgB"
     CDS             complement(<1..104)
                     /gene="ariR"
                     /locus_tag="ACHKAU_005599"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO5534400.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLG"
     gene            complement(133..405)
                     /gene="ymgA"
                     /locus_tag="ACHKAU_005600"
     CDS             complement(133..405)
                     /gene="ymgA"
                     /locus_tag="ACHKAU_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO5534401.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(448..684)
                     /gene="ycgZ"
                     /locus_tag="ACHKAU_005601"
     CDS             complement(448..684)
                     /gene="ycgZ"
                     /locus_tag="ACHKAU_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO5534402.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT          209 a          161 c          121 g          248 t
ORIGIN      
        1 cctaacactg ctgactcttc ttctaacaaa ttacccgaac tgcgaaagta acttgctaac
       61 gctttatcag ttattgcatt atgaattgta gtatcttcaa gcatggtaac ctctcatctt
      121 acttatgaaa ttttaatgta ttctgtttat tttcttacca ttgaacatac ttttattgtc
      181 agctgtcgct gttcttcggt ttggccctcc tgaatcacta ttgctaaaat cacgaattat
      241 gctgtcaatt aaatcgcgtc ggaaagcatc ttcctcagcc gatttcatta cgagtaattg
      301 cttaataaga gtctgcaatg aaaaatttat cttcatgcgc agtatctgga tcaccgcctg
      361 gccagtaatt tcatatttta tacgttcatt atcagatgtc ttcataacga cttcatagct
      421 aacctgttaa ataatcaggc tgttgcatta ttcaaaaagc aacccaatta gtgcattata
      481 atgtttctgt tcctcttccc cagtcgcgtg ttccagtcgg caaagaagtt tggcacaaag
      541 cgactttcga ctcagattac gtccatcttt caaaatctca gtcacaattt ccccgagagt
      601 ttcctgctga gtaggcaagt tagcttttgc aaagtaacga gtgattgctc ccgcagaatc
      661 taaagtcact gaattttgat gcatgctacg cctctgttaa aaatgttaat caatgttaag
      721 tgaggtaaca atagcaaac
//
LOCUS       DAZFJF010000458          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFJF010000458 DAZFJF010000000
VERSION     DAZFJF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.461"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..569)
                     /gene="fimE"
                     /locus_tag="ACHKAU_005602"
     CDS             complement(<1..569)
                     /gene="fimE"
                     /locus_tag="ACHKAU_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO5534403.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLI"
BASE COUNT          228 a          178 c          145 g          181 t
ORIGIN      
        1 tttatgagat tatttctttc ccataatccg gcaaaacgag cagcattact ggcggtataa
       61 cgtacagtat ggcgaatatt tcgatgcccg agataatcct gaattaaacg agtatctgca
      121 ccacgctccg ccagttcata accgcaagca tgccttaaca tatgaggatg agtctgcgtt
      181 acggttccag cttcaatacc ggcatcgcga ataatgcgat aggcctgctg gcgagaaagc
      241 cgactcccac ggcgagaaat aaatatagcg tcggtccggt cagcgccttt ccagttagca
      301 cgttcctggg tccagcgttc cacggcttca cgctcatcaa aacgtaacgg gtgaacggta
      361 gaaaatccgt tcttcagtcg gcgaatattt attcgacctt cattaaggtc aagatcctga
      421 taatgcagat caagcagttc actaatacgc atcccatgcc gatatgccaa cagaataaga
      481 caataatctc tggctcccgt tgccccgtaa caaaccgcct gcatcatggc ctgaacttct
      541 ttaccggtaa gataacgacg tttactcaca atagtagtac tcctgactga gatatatttg
      601 aatgaatacc tataggaaac ctcaatcggt caaaattagc ccaatagata aaaagataac
      661 cacacacgaa gtgatgtggc tatcagtcaa ttacattaaa tatcatacaa aaataaaata
      721 tcactgatgt ga
//
LOCUS       DAZFJF010000459          728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFJF010000459 DAZFJF010000000
VERSION     DAZFJF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.462"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..728
                     /locus_tag="ACHKAU_005603"
                     /pseudo
     CDS             1..728
                     /locus_tag="ACHKAU_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          191 a          201 c          198 g          138 t
ORIGIN      
        1 gacaaaaaca gtatcaacca gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag
       61 tgaagccctg aagcttgctg aacgcatcgg tgttactgcc gcagcccgtg aactcagcct
      121 gtatgaatca cagctctaca actggcgcag taaacagcaa aatcagcaga cgtcttctga
      181 acgtgaactg gagatgtcta ccgagattgc acgtctcaaa cgccagctgg cagaacggga
      241 tgaagagctg gctatcctcc aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag
      301 tatgtcttta ttgaaaaaca tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc
      361 cgggtggccc gcagcggctg gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt
      421 cagcagttcc gccaacactg cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag
      481 cgttacggtg ccccacgcct gacggatgaa ctgcgtgctc agggttaccc ctttaacgta
      541 aaaaccgtgg cggcaagcct gcgccgtcag ggactgaggg caaaggcctc ccggaagttc
      601 agcccggtca gctaccgcgc acacggcctg cctgtgtcag aaaatctgtt ggagcaggat
      661 ttttacgcca gtggcccgaa ccagaagtgg gcaggagaca tcacgtactt acgtacagat
      721 gaaggctg
//
LOCUS       DAZFJF010000460          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFJF010000460 DAZFJF010000000
VERSION     DAZFJF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.463"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>727
                     /locus_tag="ACHKAU_005604"
     CDS             <1..>727
                     /locus_tag="ACHKAU_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO5534404.1"
                     /translation="KHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVEY
                     TRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLV
                     RISGPRQTREYGYSATGRLESVRTLAPGLDIRIPYATDPAGNRLPDPELHPDSTLTAW
                     PDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHRLVFHTRIQ
                     HGEPLVESRYLYDPLGRRMAKRVW"
BASE COUNT          165 a          233 c          238 g           91 t
ORIGIN      
        1 gaaacacgcc tacaacgaac aggggctggc aaaccgcgtc acgccggaca gcctgccgcc
       61 ggtggagtgg ctgacctacg gcagcggtta cctggcgggc atgaagctgg gcgggacgcc
      121 gctggtcgag tatacgcggg acaggctgca ccgtgagacg gtgcgcagct tcggcagcag
      181 ggcaggcagt aatgccgcat atgaactgac cagcacatac acccccgcag gccagttaca
      241 gagccagcac ctgaacagcc tggtgtatga ccgtgactac gggtggaatg acaacggcga
      301 cctggtgcgc atcagcggcc cgcgacagac gcgggaatac ggctacagtg ccacgggcag
      361 gctggagagc gtgcgcaccc tcgcaccggg cctggacatc cgcatcccgt atgccaccga
      421 cccggcggga aaccgcctgc cggacccgga gctacacccg gacagcacgc tcacggcgtg
      481 gccggataac cgcatcgcag aggatgcgca ctatgtttac cactatgatg aatacggcag
      541 actgacggag aagacggacc gcatcccgac gggtgtgata cggacggacg acgagcggac
      601 ccaccactac cactacgaca gccagcaccg cctggtgttc cacacgcgga tacagcatgg
      661 cgagccactg gtcgagagcc gctacctcta cgacccgctg ggacggcgaa tggcgaaacg
      721 ggtatgg
//
LOCUS       DAZFJF010000461          717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFJF010000461 DAZFJF010000000
VERSION     DAZFJF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.464"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>717
                     /locus_tag="ACHKAU_005605"
     CDS             <1..>717
                     /locus_tag="ACHKAU_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5534405.1"
                     /translation="LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYT
                     GLPQNVDTAEDSEQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPN
                     DPKWVKGIQTRDSVNQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACT
                     ACGQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQH
                     HNSDNETGETADHPIKVNGH"
BASE COUNT          244 a          166 c          188 g          119 t
ORIGIN      
        1 cctcttcgca gataaggacg gagaaaaata cggtcgcgca aacaaacttt ctggtctgga
       61 taaggcactg gctgctggcg ccactgaaat cacaaaagaa gaatattttg cccgaaaaaa
      121 tggcacatac acgggcttac cgcaaaatgt agataccgct gaagattcag aacaaccaga
      181 gccgataaaa gttaccgctg acgaagtaaa caaaattatg caggcagcca atatcagcca
      241 gcctgacgcc gataagttgc ttgctgcatc acgtggtgaa tttgttgaag ggattagtga
      301 cccgaatgat ccgaaatggg ttaaggggat ccagacccgc gattctgtga accagaacca
      361 gcatgaatcg gaacggaact accaaaaagc ggaacaaaac agtccaaatg cgttacaaaa
      421 cgagccagaa acgaaacagc ctgaaccagt ggcgcaacag gaagtggaaa aagcctgcac
      481 cgcctgcggt cagaccggcg gcggcaactg ccctgattgt ggcgcggtga tgggcgacgc
      541 aacataccaa gaaacattcg atgaagagta tcaggttgaa gttcaggaag atgatccgga
      601 ggaaatggaa ggcgctgaac atccacacaa ggagaacact ggcggcaatc agcatcacaa
      661 tagcgataat gaaactggcg agacggcaga tcacccaatt aaggtgaacg gtcatca
//
LOCUS       DAZFJF010000462          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFJF010000462 DAZFJF010000000
VERSION     DAZFJF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.465"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>716
                     /locus_tag="ACHKAU_005606"
     CDS             <1..>716
                     /locus_tag="ACHKAU_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534406.1"
                     /translation="NSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTPQ
                     QLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMRK
                     YYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIVF
                     QDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYEY
                     KVLPPLPDKNEASTTDMTEQ"
BASE COUNT          224 a          153 c          163 g          176 t
ORIGIN      
        1 gaattcaaaa aagctttgtt gcatatgtgt gttattctcg ctgcttgcag gatgtgcctc
       61 tgaatcttct attgatgaaa agaagaaaaa agctcaagtc acacaaagta atattaataa
      121 aaacactccc cagcaactga cagacaaaga tttattcggt aatgaaacca ctctggccgt
      181 atccgaagag gatattcaag ctgcgcttga tggagatgag ttccgcgttc cccttaattc
      241 tcctgtaata cttgttcaat ccggcaaccg cgcaccggaa accattatgc aggaggagat
      301 gcgaaaatat tatactgttt ccacattctc tggtatcccg gacaggcaaa agcctctgac
      361 ttgtaacaaa aacaaagata aaaatgagaa cgaggatgtt gccagtgctg agaatatgaa
      421 ctggatgcag gcactgcgtt ttgtggctgc aaaagggcat cagaaagcga ttattgtttt
      481 ccaggatacg ttgcagacag gaaaatatga ctctgcgctg aaatcaacag tatggtccga
      541 ctataaaaat gaaaaactca ccgacgctat ctccctgcgc tacctggtac gtttcacgct
      601 ggtggatgtg gcaacaggtg agtgggctac ctggtcgccg gtgaattatg aatataaagt
      661 gcttccacca ttgcccgaca agaatgaagc cagtacgact gatatgacag agcagc
//
LOCUS       DAZFJF010000463          699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFJF010000463 DAZFJF010000000
VERSION     DAZFJF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.466"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(312..>699)
                     /locus_tag="ACHKAU_005607"
     CDS             complement(312..>699)
                     /locus_tag="ACHKAU_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5534407.1"
                     /translation="PLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRR
                     AEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDM
                     AATLVVPFTGDGRDAAEILRTLPPLK"
BASE COUNT          145 a          199 c          182 g          173 t
ORIGIN      
        1 cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact
       61 gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac gtttatattc
      121 agtttcctgc agtgctgcct gtggctgagc tgccatctgc ctgtccctta cgtgagtcac
      181 cccgtaacct gatgctgagg cattgctccc ttcataaaac atggtttact cactacagct
      241 tatctaccag ctccagctta tgttatgtct gttctgctga ccactgctta tataaggaag
      301 agcgtatgct gtcacttcag gggaggcagt gtacgcagga tctctgcagc atcgcgcccg
      361 tcacctgtga aaggcactac cagcgtggca gccatatcca gcgcaaaaac tctggtataa
      421 acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
      481 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
      541 gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
      601 gtttcgtcag tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
      661 ctttccatct gcctgacata gcttatatct gtcagcgga
//
LOCUS       DAZFJF010000464          695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFJF010000464 DAZFJF010000000
VERSION     DAZFJF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.467"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..206)
                     /gene="escT"
                     /locus_tag="ACHKAU_005608"
     CDS             complement(<1..206)
                     /gene="escT"
                     /locus_tag="ACHKAU_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO5534408.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLT"
     gene            complement(206..475)
                     /gene="escS"
                     /locus_tag="ACHKAU_005609"
     CDS             complement(206..475)
                     /gene="escS"
                     /locus_tag="ACHKAU_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO5534409.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(475..>695)
                     /gene="escR"
                     /locus_tag="ACHKAU_005610"
     CDS             complement(475..>695)
                     /gene="escR"
                     /locus_tag="ACHKAU_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO5534410.1"
                     /translation="PAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPV
                     TISIPFKILLFILVGGWQKLFEFLLVVN"
BASE COUNT          253 a          133 c          123 g          186 t
ORIGIN      
        1 gtcaactgaa aaattccgga tggtaatttt tccgaaaatg tatagttctc tactataatt
       61 ggaagtgtaa acgcaatcat aattgagttg cgtataaagt tactgagcaa aacacctgtt
      121 gaaaatattg gtagaataat aaacatcccc agcggcctga gtatacaaaa aaatgatgat
      181 actatgaccg tcattatttc attcattagc cattcacctt cggaatcatt tcaaatatta
      241 tcgacgagaa gttgatgatt gttgttccca tccagtgata agtcagggca agcgtagcaa
      301 acactgctat tatttttagc aaaaaaggca atgtttgatc ctgtaactgc gttatagcct
      361 gaaccaggct aataataatg ccgacaacag aggccgctat gactgtaggc agtgagagga
      421 taaatattat ccaaagcgtt tgcacacata attgaacaaa atatccagtt tccatcaatt
      481 caccaccaac agaaattcga ataatttttg ccagcctcca acaagaatga ataacaagat
      541 cttaaaagga atcgaaattg ttactggcga taccatcatc atacccaagg ccaataagat
      601 attggaaatg atcaaatcta tcgcaataaa aggtaaatag agcaaaaaac ctatcttgaa
      661 tgcagcttca agctgcccca tcgtgaaagc tggta
//
LOCUS       DAZFJF010000465          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFJF010000465 DAZFJF010000000
VERSION     DAZFJF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.468"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            9..>686
                     /locus_tag="ACHKAU_005611"
     CDS             9..>686
                     /locus_tag="ACHKAU_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534411.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESKSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPEN"
BASE COUNT          269 a          127 c          128 g          162 t
ORIGIN      
        1 atgtaattat gagactgcca gagaaagttc tttttcctcc tgtcactagt ggcctgtcag
       61 ggcaggaaaa acaaaaaaaa ccgaagagca ttaccggatt tcaggaaaat tatcaacgca
      121 atatcaggcc aatcaaaaca gcatcagaag cccgactacg cttctttgat aaaatggttt
      181 cgaaagaaaa ctctctggaa gatgttgttt ctttaggtga aatgattcag aaggaaattt
      241 atgggcatga acaaagaaca ttttcaccag ttcatcatac aggtaactgg aaatcatcat
      301 tgttacacaa cgcgctcctt ggtctggcaa atgtttataa tggcttacgg gaaacagaat
      361 accctaacac tttcaacaga gatggtataa aaagtactaa ctcttttaga gataacttat
      421 tgacaaaaac aagaactccc agagataatt ttgaggaagg aataaaacat cctgaacatg
      481 caacaatacc atatgacaac gacaatgaaa gtaataaatt gctaaaagca ggaaagatag
      541 ctggtaacaa taacgagctg ttgatggaaa taaaaaagga atccaaaagc gaccatcaaa
      601 tccccctgtc agataagttc ctgaaaagga aaaaacggtc tcctgtagct gaagataaag
      661 ttcaaaactc gttaacacca gaaaat
//
LOCUS       DAZFJF010000466          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFJF010000466 DAZFJF010000000
VERSION     DAZFJF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.469"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..446)
                     /locus_tag="ACHKAU_005612"
     CDS             complement(<1..446)
                     /locus_tag="ACHKAU_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO5534412.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQ"
BASE COUNT          188 a          167 c          174 g          157 t
ORIGIN      
        1 tactggacac aaaactcacg ggaaggatgc ggctcgctga acagcggact ccattcggtc
       61 accccgtttt cctgcaacat cgtgccgagg atgcttttga tgtcctcgtt ctggaagata
      121 cggaagttct ggcgcagacc ggcacgccac agcggcgggt gcaccttcat gctgtacagc
      181 atctggtttt tgtcgttctc cccgagttca aaccaggtca ccacgccttt cacccggcgc
      241 tgtacttcat cgccctgcca tatcgtcagg taggccattt tgtccagcac ctgcgcaaat
      301 tcaagggaga gaaactgctg gctgaccagg gagagatcga gggaaaagag cgaagagagt
      361 gactggttca gatgaaagga aaccaccgca aaagcgtccg gcggcaggcc gtccacttcc
      421 agtgtgaaac gtaatccggt tgacatagca acctccggta aaatgaataa tgatccgtac
      481 cgtacaactg cggataatgt gataagcggg acgacttcag acgttattag ggaaggaaca
      541 gaaaatgcta tggttatgtg agaattttgt ttaaatatta ctttacagaa acgggtgtct
      601 gttcagtcaa aaaaataatc acaagctcaa cctcttgttt cttggcgtta ttcaggacag
      661 gtacgcatat cacaacgagt aacaaa
//
LOCUS       DAZFJF010000467          677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFJF010000467 DAZFJF010000000
VERSION     DAZFJF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.470"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>677
                     /locus_tag="ACHKAU_005613"
     CDS             <1..>677
                     /locus_tag="ACHKAU_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5534413.1"
                     /translation="CGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVN
                     VPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAILRH
                     AGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGRAE
                     VVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQY
                     VMAFYWPV"
BASE COUNT          169 a          162 c          223 g          123 t
ORIGIN      
        1 tgtggagata cgacgtattt ctggaagcag aagaacgaat ccacgtacca gacggtgtac
       61 ggcagctata aaaacaaaac ggaaaagaat atccatttcg taccgttcat gaccgatgag
      121 aacggggtga atgtgccgac gaacaaaccg gaagaagacc cggacattcc gggtatcgga
      181 tattacggtt cgaaatggcg tgacagctca gccacctgga cgtcacagga cagggcgagc
      241 catttcagcg cctgggcacg ccgtgggatt atttccgacc gtctggcaac ggcgattttg
      301 cgccatgcgg gaagagtggc gctaaacgcg ggggcatcat cgacagtatc agaggtgcgc
      361 ccttcatcgc cttccggtgc agaagccaca ggcgtcacaa cactgctctc ttaccttgcc
      421 agcgagtcag agggaagcct gaaagtacag ggatggtcag ccagtggcgg cagggcagaa
      481 gtggtcagcg atgcggaggg aaccggaggt aaggcagtga agctgaccaa ggaggccggt
      541 aaaagcagct gggtgctgga gtacgccgcg ggcaacggtg cggctctgtt acagaaaggg
      601 gggcagattc gctgccgctt taaggtttcg ggagcgctgg ctgcgaacca gtatgttatg
      661 gcgttttact ggccggt
//
LOCUS       DAZFJF010000468          677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFJF010000468 DAZFJF010000000
VERSION     DAZFJF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.471"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>677
                     /locus_tag="ACHKAU_005614"
     CDS             <1..>677
                     /locus_tag="ACHKAU_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5534414.1"
                     /translation="CGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENGVN
                     VPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAILRH
                     AGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGRAE
                     VVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQY
                     VMAFYWPV"
BASE COUNT          168 a          162 c          223 g          124 t
ORIGIN      
        1 tgtggagata cgacgtattt ctggaagcag aagaacgaat cctcgtacca gacggtgtac
       61 ggcagctaca aaaacaaaac ggaaaagaat atccatttcg taccgttcat gaccgatgag
      121 aacggggtga atgtgccgac gaacaaaccg gaagaagacc cggacattcc gggtatcggg
      181 tattacggtt cgaaatggcg tgacagctca gccacctgga cgtcacagga cagggcgagc
      241 catttcagtt catgggctcg ccgcgggatt atttccgacc gtctggcaac ggcgattttg
      301 cgccatgcgg gaagagtggc gctaaacgcg ggggcatcat cgacagtatc agaggtgcgc
      361 ccgtcatcgc cttccggtgc agaagccaca ggcgtcacaa cactgctctc ttaccttgcc
      421 agcgagtcag agggaagcct gaaagtacag ggatggtcag ccagtggcgg cagggcagaa
      481 gtggtcagcg atgcggaggg aaccggaggt aaggcagtga agctgaccaa ggaagccggt
      541 aaaagcagct gggtgctgga gtacgccgcg ggcaacggtg cggctctgtt acagaaaggg
      601 gggcagattc gctgccgctt taaggtttcg ggagcgctgg ctgcgaacca gtatgttatg
      661 gcgttttact ggccggt
//
LOCUS       DAZFJF010000469          668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFJF010000469 DAZFJF010000000
VERSION     DAZFJF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.472"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>668
                     /locus_tag="ACHKAU_005615"
     CDS             <1..>668
                     /locus_tag="ACHKAU_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5534415.1"
                     /translation="ANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELNTSLKF
                     VIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSNVNRTI
                     ANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRDVTEEE
                     GNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVDALE
                     DDDD"
BASE COUNT          219 a          116 c          150 g          183 t
ORIGIN      
        1 gggccaatat tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa
       61 aattcggtgt agaaatctat gctgctgcca aacaaggtga acccgatcca gaattaaaca
      121 catctttaaa attcgttatt gaacgtgcaa agcaggcaca agttccaaag cacgttattg
      181 ataaagcaat tgataaagcc aaaggcggcg gagatgaaac gttcgtacag ggacgttatg
      241 aaggctttgg tcctaatggc tcaatgatta ttgctgagac attgacttca aatgttaacc
      301 gtacgattgc taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag
      361 gttctgtcag ctatatgttt gacaatacgg gtgtgattgt atttaaagga acagaccctg
      421 accatatttt tgaaattttg cttgaagctg aagttgatgt tcgtgatgta actgaagaag
      481 aaggtaatat tgttatttat actgaaccta ctgaccttca taaaggaatc gcggcgctaa
      541 aagcagctgg aatcactgag ttctcaacaa cagaattaga aatgattgct caatctgaag
      601 ttgagctttc cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg
      661 acgacgat
//
LOCUS       DAZFJF010000470          664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFJF010000470 DAZFJF010000000
VERSION     DAZFJF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.473"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..>664
                     /locus_tag="ACHKAU_005616"
     CDS             45..>664
                     /locus_tag="ACHKAU_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5534416.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          174 a          162 c          169 g          159 t
ORIGIN      
        1 atcctacttt tttattgcct tcaaataaat ttaaggagtt cgaaatggac cagaagctgt
       61 taacggattt ccgctcagaa ctactcgatt cacgttttgg cgcaaaggcc atttctacta
      121 tcgcggagtc aaaacgattt ccgctgcacg aaatgcgcga tgatgtcgca ttccagatta
      181 tcaatgatga attatatctt gatggcaacg ctcgtcagaa cctggccact ttctgccaga
      241 cctgggacga cgaaaacgtc cataaattga tggatttgtc gatcaataaa aactggatcg
      301 acaaagaaga atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaat atggttgccg
      361 atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt tggcaccaac accattggtt
      421 cttccgaggc ctgtatgctc ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg
      481 aagctgcagg caaaccaacg gataaaccaa acctggtgtg cggtccggta caaatctgct
      541 ggcataaatt cgcccgctac tgggatgtgg agctgcgtga gatccctatg cgccccggtc
      601 agttgtttat ggacccgaaa cgcatgattg aagcctgtga cgaaaacacc atcggcgtgg
      661 tgcc
//
LOCUS       DAZFJF010000471          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFJF010000471 DAZFJF010000000
VERSION     DAZFJF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.474"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>658)
                     /locus_tag="ACHKAU_005617"
     CDS             complement(<1..>658)
                     /locus_tag="ACHKAU_005617"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5534417.1"
                     /translation="FKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARS
                     QNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVN
                     SENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLK
                     NIDEYINNNSEDKLIVFHLMGSHGPTYYLRYPEPHKYFKPTCDRSDIENCTHEQLINT
                     Y"
BASE COUNT          200 a          131 c          105 g          222 t
ORIGIN      
        1 cataggtatt aatcaattgc tcatgcgtac aattttcgat atcactacga tcacatgttg
       61 gtttaaaata tttatgtggc tcaggataac ggagatagta ggtaggacca tgactaccca
      121 tcaaatgaaa tacgataagt ttatcctctg agttattatt tatatactca tcaatatttt
      181 ttagcataac ctcatcataa catgagtttt tcttgcataa tgtattatct gaatttgatg
      241 gctcaatatt aattgtaggg attcgcttac atacaccttt acatcccccg tcattttcaa
      301 tccagagtaa atttaccccc gttctgtaca gaatatccag tacattttcc gaatttaccg
      361 ctttgcggga attgaactct tttcgcttca tatctgaaaa catgcatgga acagatatgg
      421 ctgtggatgt gccacacgac tgcacattat gaaaggatat aagttcatta tatttctttg
      481 taaaatcatt ggtaccacga gaatatccat tcagagcata gttttgactt cttgctgtct
      541 ccccgacaac taaaaacata attaccggct tttgtttttc atttattgtg cgggatgcat
      601 ccgttccagt ctgacgaaac tcgccaggag aaacaaaaaa atcccttaca tatttaaa
//
LOCUS       DAZFJF010000472          646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFJF010000472 DAZFJF010000000
VERSION     DAZFJF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.475"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..604)
                     /locus_tag="ACHKAU_005618"
     CDS             complement(59..604)
                     /locus_tag="ACHKAU_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO5534418.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
BASE COUNT          141 a          190 c          155 g          160 t
ORIGIN      
        1 ctgtggctcc ggcctgaaca gtgagcgaag cccggcgcgg acaaaatgcc gcagcctgtt
       61 aacctgactg ttcgatatat tcactcagca accccggtat cagttcatcc agcgcggctg
      121 ctttgttcat ggctttgatg atatcccgtt tcaggaaatc aacatgtcgg ttttccagtt
      181 ccggaaaacg ccgctgcacc gacaggggga tcccgtcgag aatactggca atttcacctg
      241 cgatccgcga cagcacgaaa gtacagaatg cggtttccac cacttcagcg gagtctctgg
      301 catttttcag ctcctgtgcg tcggcctgcg cacgcgtaag tcgatggcgt tcgtactcaa
      361 tagtccctgg ctggagatct gtctcgctgg cctgccgcag ttcttcaact tcccggcgca
      421 gcttttcgtt ctcaatttca gcatcccttt cggcatacca ttttatgacg gcggcagagt
      481 cataaagcac ctcattaccc ttgccaccgc ctcgcagaac gggcattccc tgttcctgcc
      541 agttctgaat ggtacggata ctcgcaccga aaatgtcagc cagctgcttt ttgttgactt
      601 ccattgttca ttccacggac aaaaacagag aaaggaaacg acagag
//
LOCUS       DAZFJF010000473          627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFJF010000473 DAZFJF010000000
VERSION     DAZFJF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.476"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>627
                     /gene="espX1"
                     /locus_tag="ACHKAU_005619"
     CDS             <1..>627
                     /gene="espX1"
                     /locus_tag="ACHKAU_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO5534419.1"
                     /translation="RQNELVKVFCEYKTEFLILFDDFFRSHNLPKPSPVLHHFFQYTH
                     LRDAYFYRCKLIEHTVQFYFFKHKGITLLRLDVFDDRTSECLSEEIKIYQECHEKFVN
                     FLKANFNQEIYPQLYTPEIFYEACRNLQSFYDHQETSQNAKYSAIDKKKSHLNQEIKK
                     LIKKNIFPELYNEQCNKIPSSSIDDNQGIMWQNFKTSNIAYSQLCKKLS"
BASE COUNT          244 a          106 c           87 g          190 t
ORIGIN      
        1 aagacaaaat gagttagtaa aagtattttg cgagtataaa acagagttct taattttgtt
       61 tgatgacttt tttagaagcc ataacttgcc taaaccatct ccagtccttc atcatttttt
      121 tcaatatact catctgagag atgcatattt ttatagatgt aaactcatag aacatacagt
      181 tcaattttat ttttttaaac acaaaggtat cacattactt agattggatg tttttgatga
      241 cagaacaagc gaatgtcttt ctgaagagat taagatatac caagaatgcc atgaaaaatt
      301 cgttaatttt ttaaaggcaa acttcaacca agaaatatac ccacagctat atactcctga
      361 aatattttat gaggcctgca gaaatctcca gtcattttat gatcatcagg aaacgtcaca
      421 aaacgcaaaa tacagtgcaa tagacaagaa aaaaagtcat cttaaccagg aaatcaaaaa
      481 gcttattaaa aaaaatattt tccctgagct atacaatgag caatgcaata aaatcccctc
      541 ctcatcgata gatgataacc agggaataat gtggcagaac tttaaaacta gcaatattgc
      601 atattcacaa ctatgtaaaa aattatc
//
LOCUS       DAZFJF010000474          627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFJF010000474 DAZFJF010000000
VERSION     DAZFJF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.477"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>627)
                     /locus_tag="ACHKAU_005620"
     CDS             complement(<1..>627)
                     /locus_tag="ACHKAU_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5534420.1"
                     /translation="NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQF
                     STNEIAAVVAERDVEWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEM
                     LRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFI
                     HYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCA"
BASE COUNT          197 a          121 c           98 g          211 t
ORIGIN      
        1 ttgcacaata ccgatactga ttgctattta caggtttaag taaatcttct ttactccacg
       61 gagaagctgc tttggcgatc aaaaaactac gtgacaccgg atagtcagcc cactcatgcc
      121 agggttttgt aggtcccaca tagtgtataa acacggtgtc atcattgact ggattatcaa
      181 ctttgtcttt taattcatag ttaatactat atcgggtatt atatttctcc gaaataaact
      241 tcactttacc attcaataat acattcagta catcttgatc aaggtgggtg attttactta
      301 cccaatctgg gtcacgcaac atttcaatag cttttgacga aatgttattt agattccact
      361 catcaatatt tatcagtaaa aaaccagcat tgaaatatcc agaagctaac tgtggtgtag
      421 ttaataccga ggctcgattt tgccaccatt caacatctct ttcagctaca actgcggcaa
      481 tttcattagt agaaaattga taatctaaga gttctttaat actacccttg caagcaatat
      541 ctgcatcaag atatagtatt ttttcatgtt tatgatagaa ataatctgca attataaatc
      601 gaaaatatgt tgcgtaagtc cagtttt
//
LOCUS       DAZFJF010000475          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFJF010000475 DAZFJF010000000
VERSION     DAZFJF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.478"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..444
                     /locus_tag="ACHKAU_005621"
     CDS             <1..444
                     /locus_tag="ACHKAU_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534421.1"
                     /translation="TRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGVSFISM
                     MILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKETE
                     TISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            544..625
                     /locus_tag="ACHKAU_005622"
                     /pseudo
     CDS             544..625
                     /locus_tag="ACHKAU_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          200 a          114 c          107 g          204 t
ORIGIN      
        1 acaagaaaac gtattcctga actttcagca cttccacctt cattaagaaa gaaaaatttt
       61 actgacgcat ataatgcagc atcttctcat atcgaatact ggataggtgc tggcgtctca
      121 tttatctcga tgatgatttt atccagagta tatgactttc ttttacccgc ccaagatact
      181 ttcccgggtg atatcatcag aagtttatgt gttgtgtgtc catccatatt aatctggttt
      241 cagttttcgg tatatgtcat gaggaaatat tacagacata ttctggtgcg aggaaaagaa
      301 accgaaacca tttcagaaag gttgatacgt gaggctgata cacgagaata tgaactatgg
      361 cgtccggtcc gtcgcttttt ctccattgtt ttcctcattg ttctattagg ttgcattcat
      421 tcactgataa caactataaa ataaaagtta tggctgttct gatacaggaa aataaacgtt
      481 cattctatgg ttacctcttc ctgttatata gaagaatttt aaacgacaag taagggaata
      541 taaatgaaaa taacaaaaaa taaaaatgca actataaaaa acagtcatca gtcggctgct
      601 tttcaatgtt taccttatca ttttc
//
LOCUS       DAZFJF010000476          622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFJF010000476 DAZFJF010000000
VERSION     DAZFJF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.479"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..311
                     /locus_tag="ACHKAU_005623"
     CDS             <1..311
                     /locus_tag="ACHKAU_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534422.1"
                     /translation="IRAGVQYYDRQTKKKEWTNYSAVVFAKPGAQADYYRSVLVEGGI
                     VEITGENIRVDVYQGQNGQSITLELLNAKIGFAASGNGPQQQSSNQQNTPVYDDSIPF
                     "
     gene            335..>622
                     /locus_tag="ACHKAU_005624"
     CDS             335..>622
                     /locus_tag="ACHKAU_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO5534423.1"
                     /translation="MPAPLYGADDARRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISN"
BASE COUNT          191 a          141 c          148 g          142 t
ORIGIN      
        1 acatccgtgc tggcgttcag tattacgatc gccagacaaa aaagaaagaa tggacaaact
       61 acagcgccgt tgtatttgcc aagccgggag cgcaagcgga ttactaccgt agtgttcttg
      121 ttgaaggtgg cattgtggaa attaccggag aaaacatcag ggttgatgtt tatcaggggc
      181 aaaatggtca atcaatcact cttgaattac tgaatgcaaa gattggattt gcagcttcag
      241 gaaatggccc gcagcagcaa agtagtaacc agcagaacac tcctgtatac gacgattcca
      301 tcccattctg atttagaaaa ataaggattt aattatgcca gcgcctctgt atggtgcgga
      361 tgacgcgcgc cgctgttccg gcaattccgt atcggaggtg ctggataaat tcagaaaaaa
      421 ctacgatcgg ataatgtcgc taccgcagga aacgaaagag gaaaaggaat ttcgccattg
      481 tatatggctt gcagagaaag aagaacgcga gcgaatttac cagacatcaa tccgaccatt
      541 ccgcaaagcc acatataccc acttccctga aattgacccg cgcctgcgta attaccgctc
      601 acgctatggc gctatcagta at
//
LOCUS       DAZFJF010000477          617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFJF010000477 DAZFJF010000000
VERSION     DAZFJF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.480"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..598
                     /gene="ybeT"
                     /locus_tag="ACHKAU_005625"
     CDS             44..598
                     /gene="ybeT"
                     /locus_tag="ACHKAU_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5534424.1"
                     /translation="MNKKLMYIFAIFIVAAITCISHPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
BASE COUNT          203 a          136 c          127 g          151 t
ORIGIN      
        1 acttgacgct ttcaaaataa aaagaaaatc gaagcattca catatgaata aaaaattaat
       61 gtatatattc gcaatattta tagttgcagc aattacctgt attagccacc ccaagaaaac
      121 aacgttgcgt gataaagcca tggtgaatta tgcctttgat tatttaagct caccgggcag
      181 tctaccgttt accacggcgg ccacggagct ttccgcgatt catggtcact caacgtcgca
      241 atatcgcctt ggagaatttt atcttcatgg tagcgacggt aaaccactgg attatacaca
      301 ggcgagatac tggtatgagc aatcagcaga acaggaaaat ccgcgcgcac aaagcaaact
      361 ggggtggatc tacctcaaag gtctgggggt caaacccgac acccgtaaag caattctctg
      421 gtataaggaa gcagctgaac aagggtatgc tcatgctcaa tatactttag gtttgatcta
      481 cagaaatggc tcaggtatta atgttaacca ttatgaatct caaaaatggt taaaactggc
      541 cgccaaacaa cattacaaaa atgcggaaag attacttgcc gggcttcccg cacattaacg
      601 tcgactgtca accgaac
//
LOCUS       DAZFJF010000478          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFJF010000478 DAZFJF010000000
VERSION     DAZFJF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.481"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..396)
                     /locus_tag="ACHKAU_005626"
                     /pseudo
     CDS             complement(1..396)
                     /locus_tag="ACHKAU_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(405..>616)
                     /locus_tag="ACHKAU_005627"
     CDS             complement(405..>616)
                     /locus_tag="ACHKAU_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5534425.1"
                     /translation="PVIAVMLGGEAVLGLLARYASQLNAFAISLTVKSALAFLILIIY
                     FAPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          203 a          119 c          111 g          183 t
ORIGIN      
        1 ttttacggtt tttaagccaa atatacgctt taagccattt actggattca atgaatcaaa
       61 cttaaattta acagcttcag ttgctaattg cattttactt tgaaaccagg agacaagtat
      121 tgttgtgaac atgcaaacca gcaaaaaagg aatgttcgtt ttcaaccaac caacccccat
      181 agctttaaaa tagtcccaaa tatctggttt agcgttatga tcaaggatgt attcaagaat
      241 cgacataatg tgatgcacat caaaaacata tccgagatat aaagtaccca caagcattat
      301 tacagagatg gttaagtctc tactttatag aaattgccct tttttagaag catcctggag
      361 tttcttttgt gttggctttt cagttttatt ggccatgaaa cactctattt ctcaatgtat
      421 agttgcaatt gttctgggaa aaaggataac ggcattactc gttcagccag tattggggca
      481 aaataaataa ttaatattaa aaatgccagc gcacttttga cagtcagtga aatggcaaaa
      541 gcatttagtt gtgaagcata acgtgccagc aatcccagga cagcctcacc gcccagcata
      601 acggcaatca cggggc
//
LOCUS       DAZFJF010000479          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFJF010000479 DAZFJF010000000
VERSION     DAZFJF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.482"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..308)
                     /locus_tag="ACHKAU_005628"
     CDS             complement(<1..308)
                     /locus_tag="ACHKAU_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5534426.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     "
BASE COUNT          175 a          164 c           90 g          187 t
ORIGIN      
        1 ttttttgacc gcgcgtttcc ggacgtattc tgttctcctg tccctttata tcgtcggaat
       61 acccgccgct cttcaaatcc cattcccaac tcagaatgta gtctgttgac cgcttgtttt
      121 atttcggtca ggttcaccgg tgaaaccgga gtccggcgcg ccttacgcaa acactctgct
      181 cgtttctgtg ccgccacttt tcttttctgg tcatcactta gctgtaccat cacttttgcc
      241 catcgttcag ctgctctccg gtacagtcct tttttctcca gacattctgc cacgtgatca
      301 tgtagcataa gtgacctccg attatctaca gactgccatc ctgaatttac cttcccttaa
      361 tgaaataaca ataaaaaaca aaccacgcaa aaacaataaa acaacacaca aaaaaaacta
      421 aataataaac aaaaataatc accttatttt attatttttt gagggggcaa ttactgaaca
      481 aaaaacgctg actatatact caaaaccaaa caactattct gccaatcagg tatcatggca
      541 acacacggaa ttaccgtgtt tttgccttct ctgcccatac aatacgggca tatacttcat
      601 actctattgt aatatt
//
LOCUS       DAZFJF010000480          615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFJF010000480 DAZFJF010000000
VERSION     DAZFJF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.483"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..588
                     /locus_tag="ACHKAU_005629"
     CDS             <1..588
                     /locus_tag="ACHKAU_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534427.1"
                     /translation="DDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGW
                     FESGDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSE
                     ITPVTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMAIT
                     VTGVKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
BASE COUNT          163 a          130 c          199 g          123 t
ORIGIN      
        1 gatgataact acctggatga tgaagacgcg gactggactg cgaccgggca ggggcagaag
       61 tctgcaggag ataccagttt tacgctggcc tggaaaccgg gagaagaagg tcagaaaggg
      121 cttataggct ggtttgaaag cggggatgtg cgggcctata aaatccgttt cccgaacggc
      181 acggtggatg tgttccgtgg ctgggtcagc agtatcggta aggccgtaac ggcgaaggaa
      241 gtgatcaccc gtacggtgaa agtgaccaac gtgggtaaac cttctgtggc ggaagaacgc
      301 agcgaaatta cgccggtcac tgcgattaag gtgacgccga catctggtac ggtggcaaaa
      361 gggaaaacaa caaccctgac ggtttctttt gagccggaaa gtgcaaccga caaaacgttc
      421 agagcggttt ccgccgatcc gtcaacggga accattgctg tgaaagatat ggcgatcact
      481 gtgacggggg ttaaggctgg aaaagtgagt atccccgtga tttccggtaa tggtcagttt
      541 gccacggtag ctgaagtcac cgttactgaa gcgggcgctg cagggtaaac ggaggtaata
      601 catgtttctg aaaac
//
LOCUS       DAZFJF010000481          613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFJF010000481 DAZFJF010000000
VERSION     DAZFJF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.484"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>613
                     /locus_tag="ACHKAU_005630"
     CDS             <1..>613
                     /locus_tag="ACHKAU_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5534428.1"
                     /translation="KIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLV
                     GKSAFVEASGQASNDAEGYLDFFRGEIGKTHLAQGLWELIDNSQLADEMAEMKTSITE
                     TRNEITQTVNKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAG
                     IEDTDGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQLFMN"
BASE COUNT          173 a          141 c          185 g          114 t
ORIGIN      
        1 caaaaatcgc agacacatct caggtggaaa cctctgcccg ttatctgggg accggcagtc
       61 agtggagtgt atccggcccg cacattaagc ccgggaagga tttctggttt tacgtgcgca
      121 gcgtcaatct ggtgggcaag tctgcgtttg tggaagccag cgggcaggcc agcaatgatg
      181 cagaagggta tctggacttt ttcagaggag aaataggaaa aacgcatctg gctcaggggt
      241 tgtgggaact gatagataac agccagcttg cagatgagat ggcggagatg aagaccagca
      301 tcacggaaac ccgcaatgaa atcacacaga cggtcaacaa aacgctggag gaccagagcg
      361 ccaccataca gcagatacag cgcgtgcaga aggacacaaa tgatgacctg gctgcgctgt
      421 acatgctgaa ggttcaaaaa acgaaagacg gcattcccta tgtggccggg attggtgcag
      481 ggattgagga tactgatggc cagccactga gcaacatact gctgctggct gaccgtatcg
      541 cgatgataaa tccggagaac ggcaacagca cgccgttatt tgtggcgcag gggaatcagc
      601 tgttcatgaa cga
//
LOCUS       DAZFJF010000482          594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFJF010000482 DAZFJF010000000
VERSION     DAZFJF010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.485"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            311..526
                     /locus_tag="ACHKAU_005631"
     CDS             311..526
                     /locus_tag="ACHKAU_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5534429.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKVARGE"
BASE COUNT          163 a          115 c          159 g          157 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgcccact ctgaaggacg
       61 ctggctaaaa cgggtagatg tacttcacat gatacttata tttggcagta cattttctga
      121 cagacagtga cggatgttgt caagatattg tgtcatttat aacctgaacc agggggggcg
      181 gaatgttatc tggcattttt agcagagcct gaatgccata atcacggctc ccggagttgg
      241 ccgtcagtgg gtgacactgg cggctttttg ttttccttta ctttcatttt ctgtcggcgg
      301 tgacggagac atacatcaga tggaaaaaat cacaacaggt gtgtcataca ccacgtcagc
      361 ggtagggacg ggatactggt tactgcaact gctggacaaa gtctctccgt cccagtgggt
      421 ggcaataggt gtgctgggga gtctgctgtt tggcctgctg acgtatctga ctaaccttta
      481 tttcaagatt aaagaagata agcgtaaggt ggcgcgggga gagtagtcga tgaataaaca
      541 atacgaactg gttgtaaaat gaatatttct aactgaaaaa acgttccatg aggt
//
LOCUS       DAZFJF010000483          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFJF010000483 DAZFJF010000000
VERSION     DAZFJF010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.486"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>593
                     /locus_tag="ACHKAU_005632"
     CDS             <1..>593
                     /locus_tag="ACHKAU_005632"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO5534430.1"
                     /translation="NLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFS
                     GNIRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITK
                     PENPIIGNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRF
                     DSNVIDELLRIKWWNYNYSDLPDNNKCDDINYFVEEM"
BASE COUNT          191 a           84 c          121 g          197 t
ORIGIN      
        1 aatttatttc ttttctcaaa aggtgacaag ataacaattg aagataatgt tattgctgaa
       61 gaatattcaa caatgcctgt gaaaaatttc tccagtgtcg gcgctttttc atttccgacc
      121 tgccattttt cagggaatat acgtataggt cgattttgta gtattgcatc aaatgtaaaa
      181 ataatgggtg gaaatcatcc tttgaatcgt tttactactc acatgatgac ttataacggt
      241 gaatttgata agtttgctat gagtgaattt gaacgtagct ggacattgaa accattcatt
      301 accaaacctg aaaatccgat tataggaaat gatgtctgga taggaaatga tgttgtattg
      361 aaaggtggca tcgcaatagg tgatggtgct gttattgctg ccaattccgt tgttaccaaa
      421 gatgtgcccc catatgccat agttgcagga gttcctgcaa aaataatcag atttaggttt
      481 gattcaaatg ttattgatga actgttgaga ataaaatggt ggaattataa ctactcagat
      541 cttccggata ataacaagtg tgatgatata aattattttg ttgaagagat gaa
//
LOCUS       DAZFJF010000484          590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFJF010000484 DAZFJF010000000
VERSION     DAZFJF010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.487"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..590
                     /locus_tag="ACHKAU_005633"
                     /pseudo
     CDS             1..590
                     /locus_tag="ACHKAU_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          187 a          103 c          139 g          161 t
ORIGIN      
        1 attctactgt tgactatttg tgccgttatt tctggtgcag aaggctggga agatatagag
       61 gattttgggg aaacacatcc cgatgttttg aagtgatatg gtgattttga aaatggtatt
      121 cctgttcacg atatcattgc cagagttgta tcctgtatca gtcctgcaaa atttcacgag
      181 tgctttatta actggatgcg tgactgccat tcttcagatg ataaagacgt cattgcaatt
      241 gatggaaaaa cgctccggca ctcttatgac aagagtcgtc gcaggggagc gattcatgtc
      301 attagtgcgt tctcaacaat gcatagtctg gtcctcggac agatcaagac ggatgagaaa
      361 tccaatgaga ttacagccat tcctgaactt cttaacatga tggatattaa aggaaaaatc
      421 atcacaactg atgcgatggg ctgccagaaa gatattgcag aaaagataca aaaacaggga
      481 ggtgattatt tattcgcggt aaaaggaaac caggggcggc taaataaagc ctttgaggaa
      541 aaatttccgc tgaaagaatt aaataatcca gagcatgaca gttacgcaat
//
LOCUS       DAZFJF010000485          587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFJF010000485 DAZFJF010000000
VERSION     DAZFJF010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.488"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..399
                     /locus_tag="ACHKAU_005634"
     CDS             <1..399
                     /locus_tag="ACHKAU_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534431.1"
                     /translation="PFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            475..>587
                     /locus_tag="ACHKAU_005635"
     CDS             475..>587
                     /locus_tag="ACHKAU_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534432.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDT"
BASE COUNT          147 a          144 c          149 g          147 t
ORIGIN      
        1 ccatttggtg cactgacagg gcgtgtggtt ggcggtgttc tggggggaat gattgcctcc
       61 gctgtatcag gaaaaattgc cggagcgctg gttgaagaag atcgcgtcaa aattctggca
      121 atgattcagg agcaggtgac atggcttgcc ggcagtttcc tgctgaccgg acatgagatt
      181 gaaaatctga acgagaatct ggcccgtgtt atcgatcaga atgctctgga gatcattttc
      241 gccgccggta tacaacaacg ggccgcgacc aatatgttaa tcaaaccact ggtggtcagt
      301 atcatcaggc aacgccccgt catggaatat gatgcatccc atctcggcaa tatggttaac
      361 cgactggaag aagcattacc cccggagtta ccggcataac ggctattctg cgccttttac
      421 gctttatcac ctgatttgtc agtgtcatcg tcatgactca gagaggtaaa taccatgatt
      481 cacctgttca aaacctgcat gattaccgcc ttcattctgg ggttaacgtg gtctgcccca
      541 ctccgggcac aggatcaacg ttacatcagt atacgcaata cagatac
//
LOCUS       DAZFJF010000486          582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFJF010000486 DAZFJF010000000
VERSION     DAZFJF010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.489"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..193)
                     /locus_tag="ACHKAU_005636"
     CDS             complement(<1..193)
                     /locus_tag="ACHKAU_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5534433.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDG"
BASE COUNT          192 a          103 c          106 g          181 t
ORIGIN      
        1 caccatcgac aagaaagata taaccgttct tgtaaagatt atcaatagat gagtatacag
       61 acgaacgaga aagtgaaatt tcttttgcta tcttgtaacc actgctacga ccgtttttca
      121 acagactgat gtaaacctgt gcatcggtgc gagtgaaacc aaaatttatc aatttattta
      181 ccaaatgtat catagacgcg ctcctgaaat gaaaatgaaa tcttaccaat tttaacatta
      241 ttaattaaca ttttcgaaaa aattgctgag ctaaaatgtg gatttatcgt aattcatcct
      301 caagctggtt acaggtctac agtacgtaag cattgtgaac tgcgaatctg aatgccctcc
      361 aactatggtc agaagacatg ctctgaccta aatagtaggc tcactgagta agcggtgtta
      421 tcaacggttt attcatatat gctctttttt atggatggaa ttttgtaact caattacctt
      481 gataagaatc agttcgtgga gttaatgaca tttaccaata gacagttcga gcagtaagaa
      541 gccttatgga attgtaaggt ttaataccgt tattcaacct gg
//
LOCUS       DAZFJF010000487          570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFJF010000487 DAZFJF010000000
VERSION     DAZFJF010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.490"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>570)
                     /locus_tag="ACHKAU_005637"
     CDS             complement(<1..>570)
                     /locus_tag="ACHKAU_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5534434.1"
                     /translation="TFIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDITY
                     KVNGLSIIPENFFTLLSGNFKADWRWDVSKETFPKDKFAGYVKSVFSKIDFYKQCGVI
                     NPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLNHENMASSAYKHKN
                     NKVLYELLNTRDMILNELHKHVFLKDDAI"
BASE COUNT          189 a          112 c           88 g          181 t
ORIGIN      
        1 ttatcgcatc gtcctttaaa aaaacatgtt tgtgcaactc attcaatatc atgtctctcg
       61 tatttaataa ctcgtagagc actttattat ttttatgctt gtatgcagat gaagccatgt
      121 tctcgtgatt taataattca cataataaac tccaaccctt ttccctgatg cctatatgcc
      181 ctgaaacgag caatgcataa agtacagcgc ctactcttcc atctgtatcg ccaaaataag
      241 ccgtgttggc attttgtggg ttaatcacac cacattgttt ataaaagtct attttagaaa
      301 acacagattt aacatagcca gcaaacttat ccttaggaaa cgtctcttta gagacatccc
      361 aacgccagtc agctttaaaa ttaccgctaa gaagagtaaa gaaattttca ggtatgatag
      421 aaagcccatt aactttataa gttatgtctg atttatctcc agaggaatta ataaaataat
      481 aaccgccctt acactcaaca gtaacggtaa tgggagacga taattttaac acatggttat
      541 tgtctggtat aattgtaaag ataaacgtgt
//
LOCUS       DAZFJF010000488          559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFJF010000488 DAZFJF010000000
VERSION     DAZFJF010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.491"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..>559
                     /locus_tag="ACHKAU_005638"
     CDS             11..>559
                     /locus_tag="ACHKAU_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspF repeat-containing protein"
                     /protein_id="HFO5534435.1"
                     /translation="MTSAASRVSSAGLSGFSVSPQAVRLNPIRAQSPFSPGTSNINAR
                     TTFNVSSPATSFTPSRPAPPPPTSGQASGASRPLPPIAQALKDHLAAYEKSKSLDTSS
                     LKPSRPAPPPPTSGLVSGSSRSLPPIAQALKDHLAAYEKSKSLDTSGLKPSRPAPPPP
                     TSGQASEASRPLPPIAQALKDHL"
BASE COUNT          125 a          169 c          133 g          132 t
ORIGIN      
        1 aggacggcat attactagtg cggcaagccg ggtaagctct gcggggttat ctggcttttc
       61 agtgtcccct caggctgttc gtctgaatcc gattagggct caatctcctt tttctcccgg
      121 tacgtcgaat atcaatgcga gaacgacttt taatgtgagt agcccggcga cttcatttac
      181 tccctctcgt ccggccccac cgccgccgac aagtggacag gcttccgggg catcccgccc
      241 tttaccgccc attgctcagg cattaaaaga tcatttagct gcctatgaaa agtcgaaaag
      301 tcttgatact tcaagcctta agccaagccg cccggcaccg ccgccgccga caagcgggtt
      361 ggtatctggg tcatcccggt ctttaccgcc cattgctcag gcgctaaaag atcatttggc
      421 tgcctatgaa aaatcgaaaa gtcttgatac ttcaggtctt aagccaagcc gcccggcacc
      481 gccgccaccg acaagtggac aggcatccga ggcatcccgg ccattaccgc ctattgctca
      541 ggcattaaaa gatcattta
//
LOCUS       DAZFJF010000489          559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFJF010000489 DAZFJF010000000
VERSION     DAZFJF010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.492"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            69..>559
                     /locus_tag="ACHKAU_005639"
     CDS             69..>559
                     /locus_tag="ACHKAU_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5534436.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKA"
BASE COUNT          165 a          110 c          130 g          154 t
ORIGIN      
        1 ttgttattat taagtctaat gtcatttaaa ggcattatca tatttacgaa gagatcagga
       61 tgatgacgat gaaaaaaagt gtattgacgg cgtttataac tgtggtatgt gcaacgtcca
      121 gcgttatggc agctgatgat aatgctatca cggatggcaa agtaacattt aatggtaaag
      181 ttattgctcc agcttgtacc ctggtagctg cgaagaaaga ttcagtagtc acattgccaa
      241 atgtcagcgc caccaagttg caaactaatg gagcagtctc tggcgttaaa actgatgtac
      301 caattgcctt agaaggctgt gatgttactg taactaaaaa tgcgacgttt acctttagtg
      361 gtacggctga cggcgtgcaa cctacagcat ttgccaacca ggctaccacc gatgcagcga
      421 cgaatgttgc tctacaaatg tatctgccag atggttctac atcggtaact cccggtaccg
      481 aaacgtcgaa tattcagtta gcggattctg ctgagcaaac agtgaccttt aaagttgatt
      541 atatcgctac ggggaaagc
//
LOCUS       DAZFJF010000490          553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFJF010000490 DAZFJF010000000
VERSION     DAZFJF010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.493"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..553
                     /locus_tag="ACHKAU_005640"
                     /pseudo
     CDS             1..553
                     /locus_tag="ACHKAU_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT          185 a          122 c          135 g          111 t
ORIGIN      
        1 aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg gaataaacca
       61 atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt caaacggtgc
      121 aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg cggcatgttt
      181 ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggagg ttcccctaaa
      241 atgagggaca tccctcaaaa cagggggaca caaaagacac tattacaaaa gaaaaaagaa
      301 aagattattc gtccgagaat tctggcgaat cctctgacca gccagaaaac gatctttctg
      361 tggttaaacc ggatgctgca attcagagcg gcagcaagtg gggaacagca gaagacctga
      421 ccgccgcaga gtggatgttt gacatggtga agaccatcgc accatcagcc agaaaaccga
      481 attttgcagg gtgggctaac gatatccgcc tgatgcgtga acgtgacgga cgtaaccacc
      541 gcgatatgtg tgt
//
LOCUS       DAZFJF010000491          553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFJF010000491 DAZFJF010000000
VERSION     DAZFJF010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.494"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          191 a          101 c          106 g          155 t
ORIGIN      
        1 tccggcggca ggccgtccac ttccagtgtg aaacgtaatc cggttgacat agcaacctcc
       61 gtgaaaatga ataataatcc gcacatattg catgtacaac atgtcgggcg gatgagataa
      121 gcgagtcgac atctggcgtt accggggaat atcagaaaaa tgtcatggtt atgtgatgat
      181 ttagtctggg attttacttc agagaaacgg gtgtctgttt agtcaacaaa ataatcacaa
      241 acgcaacgga tgggttctcg acgttattca cgacaggctc gtacattaca gcgagtaata
      301 aaaaacctga tatgcaaagt caaaccaata ttcataatgt ctttttattc acaacaataa
      361 ataattcaat aacaatcatt gaaatgaatg atggtttgta taaggaatat acgctaccgc
      421 cccaccctga taacatgatc tgcacattgc acatcttaac gttgttaaat actggctcaa
      481 tagtggatat tttaaattag gaatttatca aattcacttg ataaatagat tgagtgaata
      541 atataacaat taa
//
LOCUS       DAZFJF010000492          546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFJF010000492 DAZFJF010000000
VERSION     DAZFJF010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.495"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..539)
                     /gene="wzy"
                     /locus_tag="ACHKAU_005641"
     CDS             complement(<1..539)
                     /gene="wzy"
                     /locus_tag="ACHKAU_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O26 family O-antigen polymerase"
                     /protein_id="HFO5534437.1"
                     /translation="MYYIIFVMVLGLWIIAFNYARANKLSNLVIVLIITTLFIINRQN
                     QDYEAYVDIFNVNELYAEIGYRWLIYGVKYLGGTHEVIIGLLGLFLGTTFLRLIQYSK
                     YTAFGLLLYMLCPMPIDIVQIRNTFLFLFVINSLIELEKEHKFNSLCFVFMAPLFHSL
                     GIVYVLAWVIIQFRTWRGY"
BASE COUNT          214 a          104 c           76 g          152 t
ORIGIN      
        1 ttataacccc tccaggtacg aaattggatt attacccacg ccaatacata aacaatgcct
       61 aaactatgga aaagaggtgc cataaagaca aaacaaagag agttaaattt atgctctttt
      121 tctaactcga taagagaatt tatgacaaat aaaaaaagaa atgtattcct gatttgaaca
      181 atgtcaatag gcattggaca caacatgtaa agcagcaaac caaatgctgt atacttactg
      241 tattgtatta atcgtaggaa tgtggtccca aggaataaac ccagcaagcc aattatgact
      301 tcatgggtac cgcctaaata cttaacacca taaattaacc agcgataacc aatctcggca
      361 taaagttcat tgacattaaa tatatcaaca tacgcttcat agtcttgatt ctgcctatta
      421 ataataaata aagtagtaat aataagcaca attaccaaat tacttagctt gtttgctcta
      481 gcgtagttga atgcaataat ccataaacct aaaaccatca caaatatgat gtaatacatt
      541 ctttcc
//
LOCUS       DAZFJF010000493          539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFJF010000493 DAZFJF010000000
VERSION     DAZFJF010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.496"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..516)
                     /locus_tag="ACHKAU_005642"
     CDS             complement(<1..516)
                     /locus_tag="ACHKAU_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO5534438.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQ"
BASE COUNT          186 a          104 c           77 g          172 t
ORIGIN      
        1 ctgcggatca ttactggcat taacaatata aaaagcaatg aaaatgatag caaaaagaaa
       61 aaaaagcatt acatacttaa acagcttact gctaaagaaa tgttgttttg tagaaataat
      121 gttattttca ataatgcctg gcaatgaata ctgccaaact tcattttttt cttttacagc
      181 aaatttgaga cctaaaactt ctatttgctg attcaatgtt aacgttctat cttcaggttc
      241 ttgtgctgtt aattctctga atgttactac cagatcaggg tcggtagtaa ttattatctc
      301 aaaactacct gtaagctcgc cataaggaac gactattgtg ttttctggta actcagaaaa
      361 agcatcactt cttcctaacg aaacatcatt accaatgata acaagtagac gcccattaag
      421 tatttcatac tcgcatccat tcaatggtcc gctcaacaaa cgaatggcat aagattccaa
      481 taagtcttgt gataagaatt tatcattatt ttccatgaat accacccgct aattccata
//
LOCUS       DAZFJF010000494          513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFJF010000494 DAZFJF010000000
VERSION     DAZFJF010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.497"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>513
                     /locus_tag="ACHKAU_005643"
     CDS             <1..>513
                     /locus_tag="ACHKAU_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534439.1"
                     /translation="ILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALS
                     ESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLAS
                     FACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKW
                     KRENMTPFMYV"
BASE COUNT          153 a           84 c          116 g          160 t
ORIGIN      
        1 atacttggag agaaatatat aaaaatcaca acatccagcc cctggctttt agaaaagctc
       61 ggtaaatata ttttctcttc acgagcgcct caagttctgg aactcgctat tggctggcga
      121 ggagctttat ctgaatcaat aaaaggagtt aagttctgta tctggttttc agtagcctgg
      181 agaaccatag aatttattat gagttcagag cgagatctgg ttaattttct tggcgatttt
      241 tcaatggatg tggccaaagc agttatagcc ggtggtgttg caaccgctat tggaagcctg
      301 gcttcttttg cctgtgttag ctttggcttt ccagtaattc ttgtcggagg agcaatttta
      361 ctgacaggga tagtgtgtac agttgtttta aatgaaatcg atgctcaatg ccatttatca
      421 gaaaaattaa aatatgcaat tagagatgga ctaaaacggc aacaggaact tgataaatgg
      481 aaaagggaaa acatgactcc atttatgtat gtt
//
LOCUS       DAZFJF010000495          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFJF010000495 DAZFJF010000000
VERSION     DAZFJF010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.498"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..438
                     /gene="escT"
                     /locus_tag="ACHKAU_005644"
     CDS             <1..438
                     /gene="escT"
                     /locus_tag="ACHKAU_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO5534440.1"
                     /translation="PSISDSSSITGVILYQFITVIFVIHGGIQSVLDKLYLSYEILPL
                     QADIAFNRALVDFLFSLWDSFIKLMLSFSIPMIIGIFLCDMGFGFLNKTAPQLNVFTL
                     SLPVKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            431..>511
                     /locus_tag="ACHKAU_005645"
     CDS             431..>511
                     /locus_tag="ACHKAU_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO5534441.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEE"
BASE COUNT          158 a           84 c           88 g          181 t
ORIGIN      
        1 ccgtctataa gtgattcatc ttctatcact ggtgttattt tataccaatt tatcactgtg
       61 atctttgtta ttcatggtgg gatacaaagc gttctggata agttgtattt atcctacgag
      121 atattaccat tacaagccga tattgcattc aatcgtgctt tagtagattt tttattttcg
      181 ctatgggatt catttattaa actgatgtta tcattttcaa ttcccatgat tatcggtatc
      241 ttcttatgtg atatggggtt tgggtttctc aataaaacag cgcctcagct aaacgtattc
      301 acattatcac tgccggtaaa aagtttgata gcaatcttta tattgttatt agttattcat
      361 gttttccctg attttattac ggcaaatatt cattctgaca taattgacag atcgttaccg
      421 agcatgatta atgagtgaaa aaacagaaaa gcccacaccc aaaaaactaa gggatctaaa
      481 aaagaagggc gatgtaacaa aaagtgaaga g
//
LOCUS       DAZFJF010000496          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFJF010000496 DAZFJF010000000
VERSION     DAZFJF010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.499"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..501)
                     /locus_tag="ACHKAU_005646"
     CDS             complement(<1..501)
                     /locus_tag="ACHKAU_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534442.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKPKQ
                     TSIEMVTLLNTLQPDGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQKISTGSINVNCPLKYIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYD"
BASE COUNT          156 a           84 c           90 g          174 t
ORIGIN      
        1 atcataataa tctaaatcga cattacgtaa gtccagagag gatagatcaa cgatatctcc
       61 agttccacaa ctaaagatta cgttcaacct cctgatttca ttgtaaatat atttcaatgg
      121 acaattaaca ttgatactgc ctgtggatat tttctggttc tttaatggtt cattaatggt
      181 aagttcgcag gtattgtccg atcgttgaat aatttgggcg ttataaccta aaaaatccag
      241 acgaagagac gagtcattat aaagaattac attttgtaag tacgctaatt ctttttgaaa
      301 atcaccagtg ataatgcatt ttccatcagg ttgtaacgtg ttaagtaaag tgaccatttc
      361 aatagaggtc tgttttggtt tttctatacc tggtgctagt gcttcgaggt tatttcctgt
      421 tgggctttca ggaatgtcat tcatttttcg acggactaaa tcactactaa tatcattacc
      481 aattgatgaa acagggaaca ttat
//
LOCUS       DAZFJF010000497          503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFJF010000497 DAZFJF010000000
VERSION     DAZFJF010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.500"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(409..>503)
                     /locus_tag="ACHKAU_005647"
     CDS             complement(409..>503)
                     /locus_tag="ACHKAU_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HFO5534443.1"
                     /translation="YNLKKFLMMDMLGVGDAININANKNIRQVC"
BASE COUNT          162 a          103 c           72 g          166 t
ORIGIN      
        1 cgttaaacac tagcttaata gtggatattt caaattagga atttattaaa ttcacctgat
       61 gaatataata agcaaatagc atagaaaata atgacaactc ccccgagggg accatttcca
      121 aagcactgca ccaccacctg gtgccggatg atcttgcttc agtggtaaga gtacctgatg
      181 cattgttccc aaaatggtgt tttcccctgc ccctccaata atgtgtagct ctcacttgat
      241 aatctattga ttaggttata ttaagaatca acatttaaaa tacatccatt ttcttgaata
      301 aatcaacatg tttattaatc aattgctgtt ttagctctga ctcatcaagc tctccatatt
      361 tgtcctacag ataattgcga ataaatacag agaacgccat ttatcctttt agcatacctg
      421 ccggatattt ttatttgcat taatattaat tgcatctcca actccgagca tatccatcat
      481 taaaaacttt ttgagattat aga
//
LOCUS       DAZFJF010000498          503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFJF010000498 DAZFJF010000000
VERSION     DAZFJF010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.501"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>503
                     /locus_tag="ACHKAU_005648"
     CDS             <1..>503
                     /locus_tag="ACHKAU_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5534444.1"
                     /translation="VAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVCI
                     RENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQY
                     LAQVKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLPPGEYTLTVRA
                     INSYGQQG"
BASE COUNT           98 a          147 c          171 g           87 t
ORIGIN      
        1 gtggccatca ctgcacaccc cgcgccggac cggatacagg tcagtaccct gccggatggt
       61 gtggagacat acggtgtgtg ggggctttcc ctgccgtcac tgcgtcgtcg cctgtttcgc
      121 tgtgtctgca tccgggaaaa cacggacggc acttttgcca tcacggcggt gcagcatgtg
      181 ccggaaaaag aagccatcgt ggataacggg gccagctttg agccgcagtc aggcaccctg
      241 aacagcgtca tccctccggc agtgcagcat ctcacggtgg aggtgagcgc cgctgacggg
      301 cagtatctgg cgcaggtgaa atgggacacg ccgcgggtgg tgaagggggt gcgcttcagt
      361 ctgcgcctga ccagcggaag cggagaagac agccgtctgg tgaccaccgc catcaccgcg
      421 gatacagagc accgtttcag cggtctgccg ccgggggaat acaccctgac ggtcagggcg
      481 attaacagtt atggccagca ggg
//
LOCUS       DAZFJF010000499          493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFJF010000499 DAZFJF010000000
VERSION     DAZFJF010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.502"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>493)
                     /locus_tag="ACHKAU_005649"
     CDS             complement(<1..>493)
                     /locus_tag="ACHKAU_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5534445.1"
                     /translation="GSTPKIKGTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDES
                     TPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARG
                     DEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGE
                     KLD"
BASE COUNT          107 a          142 c          128 g          116 t
ORIGIN      
        1 tgatcgagtt tttcgcccac ggcctcttcc caggtctcgc ccagcgtggt gttcacaaag
       61 gttttcaggc cgttgggatc tttcagtgca tccagccagt catagacaat ctgtacccag
      121 gtggtgaacg gactgtacgc cgtccagata tggaacgtga tggagcgcgg cggcggaatt
      181 tcatcacccc gggcgctgaa aaacgtcaga ccgtcacggg tccacatgcc cgtgttttca
      241 cagatccacc gcccgttgct ctggtccagt tcagactgat ggatcacgca gccatgatgt
      301 tcacagaggt agaaaacact ttcagggctg tccttctccc atttaagccc aaaaggcgtg
      361 gactcatcgc caaatttcag atactgctcc tccccacagt gcgggcaggg cacataaaaa
      421 cgcataaaat gcgccgactc gttagcggct ttttcgatct ggcaggtgcc tttgatttta
      481 ggcgtcgagc cgc
//
LOCUS       DAZFJF010000500          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFJF010000500 DAZFJF010000000
VERSION     DAZFJF010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.503"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>485
                     /locus_tag="ACHKAU_005650"
     rRNA            <1..>485
                     /locus_tag="ACHKAU_005650"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZFJF010000501          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFJF010000501 DAZFJF010000000
VERSION     DAZFJF010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.504"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>476
                     /locus_tag="ACHKAU_005651"
     rRNA            <1..>476
                     /locus_tag="ACHKAU_005651"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZFJF010000502          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFJF010000502 DAZFJF010000000
VERSION     DAZFJF010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.505"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..>467
                     /locus_tag="ACHKAU_005652"
     CDS             106..>467
                     /locus_tag="ACHKAU_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFO5534446.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVL"
BASE COUNT          133 a          117 c          105 g          112 t
ORIGIN      
        1 caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc ataccccagt
       61 tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc actgactgac
      121 gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga cggggctggc
      181 atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta tcgtattctg
      241 ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc cgaagctcgt
      301 acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg cgaacagaaa
      361 agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc acgacgctgg
      421 catgccagta ataaacaatg ggcacaatca cacagcgata aagtact
//
LOCUS       DAZFJF010000503          465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFJF010000503 DAZFJF010000000
VERSION     DAZFJF010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.506"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>465)
                     /locus_tag="ACHKAU_005653"
     CDS             complement(<1..>465)
                     /locus_tag="ACHKAU_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5534447.1"
                     /translation="WDTRPSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGA
                     ALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQM
                     KMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSQEQRERLTGWI"
BASE COUNT           88 a          152 c          121 g          104 t
ORIGIN      
        1 aatccagccg gtcagccttt cccgctgctc ctgactgttc gcgcccagaa taaaatccat
       61 cgctgactgc gtatccagct cactctcaat ggtggcggca tacatcgcct tcacaatggc
      121 gctctgcagc tgcgtgttct gcagcgtgtc gagcatcttc atctgctcca tcacgctgta
      181 aaacacattt gcaccgcggg tctgcccgtc ctccacgggt tcaaagacgt gaatgaacga
      241 agcacgaccg ccgggtaact cgcggggtat ccatgtccat ttctgcggca tccagccagg
      301 atacccgtcc tcgctgacgt aatatcccag cgccgcaccg ctgtcattaa tctgcacacc
      361 ggcacggcag ttccggctgt cgccggtatt gttcgggttg ctgatgcgct tcgggctgac
      421 catccggaac tgtgtccgga acagtcgcga gggacgggta tccca
//
LOCUS       DAZFJF010000504          462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFJF010000504 DAZFJF010000000
VERSION     DAZFJF010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.507"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..313)
                     /locus_tag="ACHKAU_005654"
     CDS             complement(<1..313)
                     /locus_tag="ACHKAU_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534448.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SE"
     gene            complement(315..>462)
                     /locus_tag="ACHKAU_005655"
     CDS             complement(315..>462)
                     /locus_tag="ACHKAU_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534449.1"
                     /translation="LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQ
                     RIWR"
BASE COUNT          107 a          124 c          132 g           99 t
ORIGIN      
        1 tttcactgtg gtgataatca ccgtgtggcc actggcagga tttaacatgg cgcagtaacc
       61 agtcagacaa tgcgccagcg ccaccagcag cacgaatcac tcgttcgtcg ctgaaaaatg
      121 gcagtaatga tttatcctcc gccagcggct ggcgaacggc aggaacgacc ccggacggca
      181 gattacgcat gcttttcggt tccggctcca ccagtacccg ggtattgtgg aataccggca
      241 tggattcacg gcccggctta acgatcacca gcccgagttc cggtaccaga acaggtcgaa
      301 gtaatacccg cacgttacct ccagatgcgt tgctggaatg tgcgggacgg acgcggtggt
      361 cgttcggagt aaggaagcct gacggagatt atccagtgac gataatcgag gctgagggct
      421 ttcttaatct cgtatccgtg tctgcggtag cactgaatta gc
//
LOCUS       DAZFJF010000505          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFJF010000505 DAZFJF010000000
VERSION     DAZFJF010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.508"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            90..>457
                     /locus_tag="ACHKAU_005656"
     CDS             90..>457
                     /locus_tag="ACHKAU_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5534450.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEP"
BASE COUNT          118 a           96 c          143 g          100 t
ORIGIN      
        1 gggggtgagc ttacggagtg gtcagtgtga ctttctctgt gggcagatcg ctcccgggcg
       61 ttggctcacc cacccaaagg aacgtcacga tgtttggaat cttcaaaaag aaaacccgca
      121 gagcggcagc ggaaattaaa aagtttgaga aacgcgatct ggcacaggcg gtgattaacg
      181 ctgcatacct ggtggcctat gcagatggtg aatgcgaggc atccgagaaa gcgaagatcg
      241 aacaggtctt acgtaatcag cctgcgttgt ctgcgtttac ctcggaaatt aatgcgatta
      301 gcgcaactat tatcggtcag ctggatacca attttaaaat tggtcgtcgt gccgcgttac
      361 gcgagattga ggatgtgaaa cacgatacgc gtgaagcgga agatgtgctg gatgtggcgg
      421 tggccattgc ggaggcagac ggcgaaattg agccgga
//
LOCUS       DAZFJF010000506          447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFJF010000506 DAZFJF010000000
VERSION     DAZFJF010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.509"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            151..>447
                     /gene="grlR"
                     /locus_tag="ACHKAU_005657"
     CDS             151..>447
                     /gene="grlR"
                     /locus_tag="ACHKAU_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO5534451.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGNEKLF"
BASE COUNT          155 a           59 c           98 g          135 t
ORIGIN      
        1 accacggaaa acaacaggtt ggaatatgag tatatttaat gatatattac attgcaatct
       61 ggagaaaaag aaaggtctcc gttattcttg atatcgctta tggatagaac aaattgaaag
      121 gagtgaggtt agtatgaaac tgagtgagtt atgattatga aggatggcat ctatagcatt
      181 atctttatta gcaatgaaga ttcctgtggg gaaggtatac tgattaaaaa tggaaatttg
      241 atcactggcg gagatattgc ttctgtgtat cagggggtcc tgtctgaaga ggaggacatc
      301 atacttcatg tccatcgcta taatcatgaa attccctcgg tgctaaacat tgaacaagat
      361 tatcaattag ttatccctaa aaaagtactg agtaatgata gtaatgtcac attacattgc
      421 catgtaagag gaaatgaaaa attgttt
//
LOCUS       DAZFJF010000507          444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFJF010000507 DAZFJF010000000
VERSION     DAZFJF010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.510"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..227)
                     /gene="tnpB"
                     /locus_tag="ACHKAU_005658"
     CDS             complement(<1..227)
                     /gene="tnpB"
                     /locus_tag="ACHKAU_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5534452.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGR"
     gene            complement(224..>444)
                     /locus_tag="ACHKAU_005659"
     CDS             complement(224..>444)
                     /locus_tag="ACHKAU_005659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534453.1"
                     /translation="STTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLNISCE
                     VTFRHGSLRLNGAISENILNLLIRELKR"
BASE COUNT           95 a          114 c          126 g          109 t
ORIGIN      
        1 aagcggccgc gctccagccg tttggtcagc agacacagtc catcgccggt agaccagagg
       61 agctttacct gactgccatt acgcccacgg aagataaaaa cgtgacctga catcggatcg
      121 tctttcagcg tcgtctgcac ctttgccgcc aggccgttga agccgtttct catatcggtg
      181 atgccagcga ccagccagat ctttgtccct gatggtaatg ggatcaacgt ttgagctccc
      241 gtatcagcag gttcaggata ttttcgctga tggcaccatt cagacggagt gatccgtgcc
      301 ggaacgtcac ttcacagctg atattgagtg attcaggtgt cgcagcagct acaggttgtg
      361 accgggggga ggttgtaggg acaacatctt cggcatcaag tgttatcggg agcagctcag
      421 gcacgttgtt ttctgttgtt gaag
//
LOCUS       DAZFJF010000508          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFJF010000508 DAZFJF010000000
VERSION     DAZFJF010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.511"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>438
                     /locus_tag="ACHKAU_005660"
     CDS             <1..>438
                     /locus_tag="ACHKAU_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5534454.1"
                     /translation="LSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERT
                     HRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKP
                     QVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGE"
BASE COUNT          103 a          136 c          131 g           68 t
ORIGIN      
        1 ccctcagcat gtggccggat aaccgtatcg cccgtgacgc gcactatctt taccggtatg
       61 accgtcacgg caggctgacg gagaaaaccg acctcatccc ggaaggggtt atccgcacgg
      121 atgatgagcg cacccaccgg taccattacg acagtcagca ccggctggtg cactacacgc
      181 ggacacaata tgcagagccg ctggtcgaaa gccgctatct ttacgacccg ctgggccgca
      241 gggtggcaaa acgggtgtgg cgacgtgaac gggacctgac gggctggatg tcgctgtcac
      301 ggaaaccgca agtgacctgg tacggctggg acggcgaccg cctgaccaca atacagaacg
      361 acagaacccg catccagacg atttatcagc cggggagctt cacgccactc atcagggttg
      421 aaaccgccac cggtgagc
//
LOCUS       DAZFJF010000509          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFJF010000509 DAZFJF010000000
VERSION     DAZFJF010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.512"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>437
                     /locus_tag="ACHKAU_005661"
     CDS             <1..>437
                     /locus_tag="ACHKAU_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534455.1"
                     /translation="YLTILLAFERRTSLTSILATLYKMLISEREVSEYECQLLYLTNP
                     IDVMNILNKYIYYFPNENSPFYTLKIDSALSWDAIDAIRDYSISDIYLYPEQKTINCV
                     VEIENIVFGGYIYTLNNGVTLQNIENSLKDSSCHYVLNGYTEF"
BASE COUNT          160 a           74 c           63 g          140 t
ORIGIN      
        1 attatctcac tatattactg gctttcgaaa gacgaacttc attaacaagc atcctggcaa
       61 ctttatataa aatgctgata agcgaaagag aagtttcaga atatgaatgc cagttattat
      121 atttaaccaa cccaatagat gtaatgaata tactgaacaa atacatatat tactttccta
      181 atgagaactc accattttat acactgaaaa ttgacagtgc attatcgtgg gatgccattg
      241 acgcaatacg agactatagt atttctgata tttatcttta tcctgaacaa aaaacaataa
      301 attgtgtcgt tgagattgaa aacattgtct ttggcggtta catttataca ttgaacaacg
      361 gcgtcacatt acaaaacata gaaaactctt taaaagattc ttcatgccat tatgtcttaa
      421 atggctatac agaattt
//
LOCUS       DAZFJF010000510          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFJF010000510 DAZFJF010000000
VERSION     DAZFJF010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.513"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..270)
                     /locus_tag="ACHKAU_005662"
     CDS             complement(<1..270)
                     /locus_tag="ACHKAU_005662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534456.1"
                     /translation="MSSSGQSLALFYHDVGVFISSTRLSISTPYAAFFVTSVSISTML
                     THDVEPVIDTLKSVRRLFSNQVTASCLRLSAGVIEELPVNDGDIMT"
BASE COUNT          124 a           89 c          102 g          116 t
ORIGIN      
        1 tgtcatgata tcgccgtcgt taacaggtaa ttcttctatg actccagcag acaggcgcag
       61 gcaggaggcg gtcacctgat tactgaacaa ccttctgact gacttcagcg tgtcaatcac
      121 aggctccacg tcatgcgtca gcatagtgct gatgctgacg gatgttacaa aaaatgcagc
      181 gtatggcgtt gagatagaaa gtctggtgct ggagataaat acacctacat catgataaaa
      241 aagggccagc gactgacctg aagaagacac tggctaaaag gctttgcatg tttgtagata
      301 cttatttttc acagacagca atgatgcctg tcaatatatt attaatatgc tgattgtttc
      361 agttacagat gctttattaa gggaaaatgt cacgatcggt gcaaaagtgg tttgaaccgc
      421 cccggttttc c
//
LOCUS       DAZFJF010000511          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFJF010000511 DAZFJF010000000
VERSION     DAZFJF010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.514"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(192..428)
                     /locus_tag="ACHKAU_005663"
                     /pseudo
     CDS             complement(192..428)
                     /locus_tag="ACHKAU_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971150.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          132 a           77 c          109 g          110 t
ORIGIN      
        1 gtttttaggg ttgttttcaa tcagctatag atagggcttg gcgccagaat ggagaatatt
       61 taacgaggaa tagaagcttt gtatcagacg ggcttcccta aatagagaag ttaaataatc
      121 aggatgatgc taatgaatat cagggacaag gtgagtgtct tcatgttcag tgaaacaggg
      181 tgtcacaaaa aagattgggg ggggctagag ccttctgcac gaaaatagtg atgcgacaga
      241 tctgagatag ctttgtatcc ttctgcctgg ttcaggcctg gagcagtcca tgttttacaa
      301 aaatactcgg cagcgtaacg tgttgcaaga gcggcatcag acgaggaacc tgaggttccg
      361 ctcatgaccg ataatctcat ccgaataaaa atataccaat tggtatacca agtttatacc
      421 aagagcca
//
LOCUS       DAZFJF010000512          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFJF010000512 DAZFJF010000000
VERSION     DAZFJF010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.515"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>421
                     /locus_tag="ACHKAU_005664"
     CDS             <1..>421
                     /locus_tag="ACHKAU_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5534457.1"
                     /translation="VAERPDQLWVADFTYVSTWQGFVCVAFIIDVFAGCIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTERLKEAKLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWY"
BASE COUNT          101 a           93 c          131 g           96 t
ORIGIN      
        1 cgtggcagaa cgtcctgacc agctgtgggt ggctgatttt acttacgtca gcacatggca
       61 gggcttcgtc tgtgtggcgt tcatcattga tgtgtttgcc ggatgtatcg tggggtggcg
      121 agtctcatcg tctatggaaa cgacattcgt gctggatgca ctggagcagg cgttgtgggc
      181 ccgtcggccg tccggcacag tccatcacag tgataaaggt tctcagtatg tatcactggc
      241 ctatacggag cgactaaaag aagcaaaact gctggcatca acagggagta caggtgactc
      301 gtatgacaac gcgatggcgg agagcatcaa tggtctttac aaagcggagg taatacaccg
      361 taagagctgg aaaaaccgtg cagaagtgga actggccaca ctcacgtggg tggactggta
      421 t
//
LOCUS       DAZFJF010000513          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFJF010000513 DAZFJF010000000
VERSION     DAZFJF010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.516"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>415
                     /locus_tag="ACHKAU_005665"
     CDS             <1..>415
                     /locus_tag="ACHKAU_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5534458.1"
                     /translation="REYDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQ
                     MTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRHYDNRGRLTSVKDAQ
                     GRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKAV"
BASE COUNT           98 a          120 c          137 g           60 t
ORIGIN      
        1 ccgggaatat gatgaaccgg gcaggctggt atcggagaca tcgcgcagcg gggagacagt
       61 acgctaccgc tacgatgacg cgcacagtga gttaccggcg acgacaacgg atgcgacggg
      121 cagcacccgg cagatgacgt ggagccgcta cgggcagttg ctggcgttca ctgactgctc
      181 gggctaccag acccgctatg aatacgaccg cttcggccag atgacggcgg ttcaccgcga
      241 ggaaggtatc agcctttacc gccactatga caaccgtggc cggttaacct cggtgaaaga
      301 cgcacagggc cgtgaaacgc agtatgagta caacgccgca ggcgacctga ctgccgttat
      361 caccccggac ggcaaccgga gcgagacaca gtacgatgcg tggggaaagg cggtc
//
LOCUS       DAZFJF010000514          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFJF010000514 DAZFJF010000000
VERSION     DAZFJF010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.517"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..355)
                     /locus_tag="ACHKAU_005666"
                     /pseudo
     CDS             complement(59..355)
                     /locus_tag="ACHKAU_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT           97 a          110 c           86 g          121 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 gattacgctg aacttcacgt gagatcgtcg aaggactgcg attcagcgca gtagctatcg
      121 cacgaatgct cattttggct gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca
      181 ggtgagctac agcccgctta cgctcatggg gttttatgcc gccagtatcc cttaacatag
      241 tgaagatcgt tccgggtttt gaacccagga tattcgctat ttcactgaag cctgttccgt
      301 tcttccatag ttcaaaaaca gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa
      361 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atct
//
LOCUS       DAZFJF010000515          409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFJF010000515 DAZFJF010000000
VERSION     DAZFJF010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.518"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..348)
                     /locus_tag="ACHKAU_005667"
     CDS             complement(<1..348)
                     /locus_tag="ACHKAU_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO5534459.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEA"
BASE COUNT          133 a          109 c           67 g          100 t
ORIGIN      
        1 agcctcaagc gggcactgaa gtctctctgg acaaacagaa aacttacatt gagatatttt
       61 gttctgaata ctctcttgcg attgtgcttc acctgagtcc gaaagcatac ttagcattct
      121 ctcaccaagg gctcgaggac cactattaaa tcccaaaagc aataactcag aaagcaatcc
      181 gccattaccg ccactggatg aaaagcgccc attagtagaa tcatacacaa tactgacagt
      241 ttcaccaccg actgtaatat ctccgcgccc attggctaca attcgggcac gaacaacctc
      301 caccccaaga ttaaatgaat gtgatctaat atctgaggtt aatggcataa caaagctccc
      361 tattttaatt tgaactccag acttaaatag ctgtaacaaa catccccca
//
LOCUS       DAZFJF010000516          407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFJF010000516 DAZFJF010000000
VERSION     DAZFJF010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.519"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          101 a           96 c           92 g          118 t
ORIGIN      
        1 ttttattagt tatcaagata atttgaccct ttgctagccg ttcccctatc atcatgatag
       61 taagccgtat gtttagatgg tttatgtctg agattttgag tatcaaatct ttgggtctcg
      121 taaaaaggtt tgaatcgcta aggataatct ggacgtctcc agggtcgtta attagactga
      181 ccccaccccg atagacgatc actcccctat agtaataagc accatttcgg acttccgctt
      241 ctcgctcaca acggaccttc agctcagccg ctccggccgc ttcatgcaat gaatgtacgc
      301 agccagtcag aatattaagg tgcggatttc agttcacttg tcagccctgt gatgcaacaa
      361 gttgcggctc cggttcgtgg gagagatgaa acgacggtag ctgaagt
//
LOCUS       DAZFJF010000517          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFJF010000517 DAZFJF010000000
VERSION     DAZFJF010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.520"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>403)
                     /locus_tag="ACHKAU_005668"
     CDS             complement(<1..>403)
                     /locus_tag="ACHKAU_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO5534460.1"
                     /translation="LQYNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAE
                     RGFFYKVPAVLRDYLSAIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQ
                     QRFNLLFPDFVDHIQLPLPLASTLLERITFYA"
BASE COUNT          142 a           65 c           75 g          121 t
ORIGIN      
        1 tcgcataaaa tgtaattctc tctaaaagag ttgatgcaag tggtaacggc aattgtatgt
       61 gatcaacaaa atcaggaaat aataaattaa atcgttgctg cattgccaat ggttgttgcc
      121 ttatataggg tgacaacgtt gaaaaaccac atgtgatgat attgtgatag ctagcgatgc
      181 cgggtttgta tcgtgccttt tcatttattt tcaccggtat agctgataaa taatccctta
      241 gcacagcagg aaccttataa aaaaatccgc gttctgcaaa cctttctcga taaaagtgat
      301 aaccactgaa taaacagaac aatggaaata aattccaatt gtttatgtag taaataagtg
      361 aactattcag attaaaatgt tcaactgaaa gattgtactg cag
//
LOCUS       DAZFJF010000518          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFJF010000518 DAZFJF010000000
VERSION     DAZFJF010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.521"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>403
                     /locus_tag="ACHKAU_005669"
     CDS             <1..>403
                     /locus_tag="ACHKAU_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO5534461.1"
                     /translation="PGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEH
                     YNVLEQDLSPSGPLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISS
                     WYTDEYKKLFALPDESELQQKDAEELFDWLI"
BASE COUNT          122 a           75 c           95 g          111 t
ORIGIN      
        1 ttccgggagg atcattaacg gcatttcatg aacgattaat ggaactggcc agaggcagta
       61 ttgaaaaatc cagtgaggat gaacggtatt gtctgcattt ttcgcctgaa gcgcaaaaaa
      121 tatttattga gcactataat gttctggagc aggatttatc cccttccgga cccttaagcc
      181 cgtttcgggg acatgtttca aaaaaaacag aaaacattgc aagaattgca gcgttatttc
      241 agtatttcag ctatggtgaa gggaaaatct ctgccgatat catgacgtct gctgtcgtaa
      301 taagttcgtg gtacacagat gaatataaaa aattatttgc gttacctgat gaatctgaac
      361 tgcagcagaa ggacgccgag gagctgttcg actggctcat tga
//
LOCUS       DAZFJF010000519          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFJF010000519 DAZFJF010000000
VERSION     DAZFJF010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.522"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..375
                     /locus_tag="ACHKAU_005670"
     CDS             <1..375
                     /locus_tag="ACHKAU_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HFO5534462.1"
                     /translation="WLGLTDPNAAVYTFAGHVFNWVGVTHIIFSIVFAVGYCVVAEVF
                     PKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDLPWYENVSEIFGHLVWFWSIEII
                     RRDLRNRITHEPDPEIPLGSNR"
BASE COUNT           92 a           77 c           89 g          136 t
ORIGIN      
        1 tggttggggc tgacagatcc caatgcggct gtttatacct ttgccgggca tgtctttaac
       61 tgggttggtg ttacgcacat tatcttttcg atagtgtttg ctgtcggtta ttgtgtggtc
      121 gctgaagtat ttccaaaaat taaactctgg cagggcttac tggcaggtgc tttagcccaa
      181 ctttttgttc atatgatttc attccctctc atgggactga cgccacctct gtttgatctc
      241 ccgtggtatg agaatgtttc tgaaattttt ggacatttag tctggttctg gtctattgaa
      301 attattcgca gagatttacg aaacagaatt actcatgagc cagaccctga gatcccttta
      361 ggctcaaaca gataatgcat tgaatgataa aaat
//
LOCUS       DAZFJF010000520          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFJF010000520 DAZFJF010000000
VERSION     DAZFJF010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.523"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..83
                     /locus_tag="ACHKAU_005671"
     CDS             <1..83
                     /locus_tag="ACHKAU_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HFO5534463.1"
                     /translation="QLIIAVVAVGKRERSDVYNLASERMR"
     gene            88..360
                     /locus_tag="ACHKAU_005672"
                     /pseudo
     CDS             88..360
                     /locus_tag="ACHKAU_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558210.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          111 a           66 c          105 g          111 t
ORIGIN      
        1 aacaattaat tatcgctgtt gtagctgtgg gtaaacgtga gcgtagtgac gtttataatc
       61 ttgccagtga aagaatgaga taattaacgg cggattatcg gttcagccct ctcattaacc
      121 gctgatgctg agttggtgtg tatggctttg cgtaatgcac tggaaacacg accgcgtgat
      181 ggtcgcctgc tgtttcattc agatcaaggt gttcagtata aaagcaataa atacagaaag
      241 ttactctggc gttatggggt aatgcaaagt atgagtcgca gagggaattg tctggataat
      301 tcgccgatgg aaaaagtctt tcgcagcctt aaaagtgaat ggcttccgaa aggtggttat
      361 ggtgattgcc atgcagtacg cgaataaatc agt
//
LOCUS       DAZFJF010000521          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFJF010000521 DAZFJF010000000
VERSION     DAZFJF010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.524"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>392)
                     /locus_tag="ACHKAU_005673"
     CDS             complement(<1..>392)
                     /locus_tag="ACHKAU_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
                     /protein_id="HFO5534464.1"
                     /translation="NIPVWMHLFNIINNLDGVKLGFIISLPVFLISALNFVFTPFSFR
                     YIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQNIFETNAGEMTSYFNMSVVLWFLF
                     TGILPCGLLLLVNIRYPETWIKGIIYRL"
BASE COUNT          163 a           65 c           56 g          108 t
ORIGIN      
        1 ttaaacgata tataataccc tttatccatg tctcaggata acggatattt actaacaaca
       61 atagtccaca tggaagtata cccgtaaata aaaaccacag tacaacagac atattgaaat
      121 atgatgtcat ttcacctgca tttgtttcaa aaatattctg catcattgtt ttatcaaact
      181 gcacaccata tttcatggtg gcatatgtaa caatggaact acagataaac agaatacaaa
      241 aaaaaggttt aaatatatac ctgaatgaga acggtgtaaa aacaaaattt aatgcagata
      301 tcagaaagac tggaagagat ataataaatc caagtttcac accatcaagg ttattaataa
      361 tattaaacag atgcatccag acagggatat tg
//
LOCUS       DAZFJF010000522          391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFJF010000522 DAZFJF010000000
VERSION     DAZFJF010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.525"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>391
                     /locus_tag="ACHKAU_005674"
     CDS             <1..>391
                     /locus_tag="ACHKAU_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534465.1"
                     /translation="TVEEDGIKIFALQYIAFHQTEHYAWLVPAHWIVNFKPAPNQWLQ
                     EWKQRRNRYAIKKVAKNAERSFAFPTKKLAIESLLRRKKYHLMRIKQDLAVVSTLVDG
                     MKNIDTSTPDIEYNFGHNQETENWVFY"
BASE COUNT          151 a           56 c           76 g          108 t
ORIGIN      
        1 cgacagttga agaagatggg ataaaaatat tcgcacttca atatatagct tttcatcaga
       61 cagagcatta tgcatggttg gttcctgcgc attggattgt taattttaaa ccagcaccaa
      121 atcagtggtt acaggaatgg aaacaaagga gaaatagata tgcaattaag aaagtagcaa
      181 aaaatgcaga aagatctttt gcattcccaa cgaagaaact tgctattgaa agtttattgc
      241 gccggaagaa ataccattta atgagaataa aacaagattt ggctgttgta tcaactcttg
      301 ttgatgggat gaaaaatatt gatacatcaa caccagatat tgaatataac tttggacaca
      361 accaagaaac agaaaattgg gtattttatt a
//
LOCUS       DAZFJF010000523          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFJF010000523 DAZFJF010000000
VERSION     DAZFJF010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.526"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..346
                     /gene="cesT"
                     /locus_tag="ACHKAU_005675"
     CDS             <1..346
                     /gene="cesT"
                     /locus_tag="ACHKAU_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO5534466.1"
                     /translation="DANDEYMMIYGVCGKFPTDNPNFALEILNANLWFAENGGPYLCY
                     ESGAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEI
                     SSSDNKHYYAGR"
BASE COUNT          132 a           57 c           76 g          116 t
ORIGIN      
        1 tgatgctaat gacgaatata tgatgatcta tggcgtctgt gggaaattcc cgacagataa
       61 tcctaacttc gctcttgaga ttttgaatgc aaacttatgg tttgcagaga atggtggacc
      121 atacctgtgt tatgaatcag gagcacaatc gctgttgtta gcgttacgtt ttccgctcga
      181 tgatgctaca cctgaaaaac ttgagaatga aatagaagtc gtcgttaagt caatggaaaa
      241 cctgtatttg gtattacata atcagggaat aacattagaa aacgaacata tgaaaataga
      301 ggaaattagt tcaagcgata ataaacatta ttacgccgga agataaaaga ttatttatta
      361 atataattta ctattcattc t
//
LOCUS       DAZFJF010000524          379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFJF010000524 DAZFJF010000000
VERSION     DAZFJF010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.527"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..>379
                     /locus_tag="ACHKAU_005676"
     CDS             16..>379
                     /locus_tag="ACHKAU_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5534467.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQ"
BASE COUNT          101 a          100 c          124 g           54 t
ORIGIN      
        1 cggagaggtg gaattatgac ggcacagata gccgcttacg ggcggctggt ggacgacccg
       61 caggtaaaac agaccagcaa gggcacaccg atgacgctgg cgcgtatggc ggtatctttg
      121 ccatgcagcc aggcacagga cggacaggcg acgttatggt tatcggtcat cgcatttggc
      181 aaacaggccg acttcctggc taaacatcaa aaaggcgatg ttgccagcgt atccggcacg
      241 atgcaggtaa gccagtggac cggacagaac ggagaaacgc ggcagggctg gcaggttatc
      301 gcagacagcg taatcagtgc ccgcgcggca cgtccaggcg ggaacagacg caaaaccaca
      361 ggcacacagg gtaatcagc
//
LOCUS       DAZFJF010000525          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFJF010000525 DAZFJF010000000
VERSION     DAZFJF010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.528"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>366
                     /locus_tag="ACHKAU_005677"
     CDS             <1..>366
                     /locus_tag="ACHKAU_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534468.1"
                     /translation="KYFNDIVPMILALDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDD
                     IYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINT
                     CKETCKHLLYLSNEDKEKI"
BASE COUNT          135 a           69 c           58 g          104 t
ORIGIN      
        1 aaaatatttt aatgacattg taccaatgat tctcgcactc gacatcagga acacatatcg
       61 cccggataat gagatggcat tttactatca tattcattct tttctcactc agataccgga
      121 taatgaggat gatatatatc atgctgcaag gacatatctg cgaaattacg ttaagttatg
      181 tttatccgga tacacgccag cgaatgcgca tttcaaagat atctttgatg gcgtatatga
      241 attcattcgt aatattcgca aaaacagtac accaggaaaa acaaaactta tcgcaactat
      301 caacacatgc aaagaaacct gtaaacatct gctttattta agtaatgaag acaaggaaaa
      361 aataat
//
LOCUS       DAZFJF010000526          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFJF010000526 DAZFJF010000000
VERSION     DAZFJF010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.529"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..>364
                     /locus_tag="ACHKAU_005678"
     CDS             173..>364
                     /locus_tag="ACHKAU_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5534469.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGT"
BASE COUNT           92 a           89 c           91 g           92 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacc
//
LOCUS       DAZFJF010000527          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFJF010000527 DAZFJF010000000
VERSION     DAZFJF010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.530"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..212)
                     /locus_tag="ACHKAU_005679"
     CDS             complement(<1..212)
                     /locus_tag="ACHKAU_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5534470.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRA"
BASE COUNT           98 a           92 c           69 g          100 t
ORIGIN      
        1 gccctgtaat cctcaacctc tttacggagt ttttcatttt cgatatcagc ttccctctgc
       61 gcataccact gaattgccgt ggcggtatca aatacagatt caacgccctt accacctccg
      121 gagacgcaag ggagcccctg agactgccag cgttcaatcg ttcgcggatc cacgttgaaa
      181 atttcggcaa gtttcttttt attaaccttc atgaaacagt ctcacaacaa atacaggatc
      241 cgacatgaaa gtgcccgaaa atgacttttt caggcgtttt catgtcggac ctttacggat
      301 tcgatattag aaaaaacaaa tagttatgtt cgagaagtac cgacatgatt ttccccgga
//
LOCUS       DAZFJF010000528          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFJF010000528 DAZFJF010000000
VERSION     DAZFJF010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.531"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>355
                     /locus_tag="ACHKAU_005680"
     CDS             <1..>355
                     /locus_tag="ACHKAU_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534471.1"
                     /translation="LADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKTLFRNRLN
                     LSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICASCGLTEVHHAPENHKPD
                     PEWYCSSLCRETETLC"
BASE COUNT           93 a           86 c           88 g           88 t
ORIGIN      
        1 actggcagac ggaaactggt tactgaacaa tgcctggtat gaaagaaaca tggcagggtt
       61 taacgaacag ttgaaagaga acctgtcatt cacacctgat gaactgaaaa cgctcttccg
      121 gaaccgcctg aatttatcac cggaagcgaa tgacgatttt ctcgatcgtt gcctggacgg
      181 cggtgactgg tatccctttt cagaaggtcg ccgttttgta tcattccatc acgtggatga
      241 gcttcgtatc tgtgcctcct gcgggctgac agaagtacat catgccccgg aaaatcataa
      301 gccggatccg gaatggtact gctcctctct ttgtcgcgaa acagaaacac tgtgt
//
LOCUS       DAZFJF010000529          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFJF010000529 DAZFJF010000000
VERSION     DAZFJF010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.532"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>355
                     /locus_tag="ACHKAU_005681"
     CDS             <1..>355
                     /locus_tag="ACHKAU_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5534472.1"
                     /translation="LSALQRIEHLALLKRRAEQAEASGNLQVSVEDLVRTGAFLVAMS
                     LWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAEDVVLRLSGMSGAVHVDTDIT
                     EVAKNNALTDDDFSAG"
BASE COUNT           81 a           86 c          113 g           75 t
ORIGIN      
        1 ctgtctgcgc tgcagcgtat cgagcatctt gccctgctga aacgacgggc agaacaggct
       61 gaagccagcg gtaacctgca ggtgagcgtg gaagaccttg tcagaaccgg cgcgtttctg
      121 gtggcgatgt ccctgtggca taaccatcca cagaaaacgg agtcaccatc aatgaatgag
      181 gctgtgatgc agatcgaaca ggaggtgctc accacctggc ctgctgatgc cattgcccgg
      241 gcggaagacg tggtgttgcg tctgtccggg atgagcgggg ctgttcatgt ggatacggat
      301 atcaccgaag tggcgaaaaa taacgcgctt actgatgatg atttttctgc gggaa
//
LOCUS       DAZFJF010000530          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFJF010000530 DAZFJF010000000
VERSION     DAZFJF010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.533"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          105 a           59 c           87 g           77 t
ORIGIN      
        1 ataaaagcga aaaaatcccg cagtgctcac ggacaggaac tgcgggagcg ttacgaagat
       61 tgagtgtaag gaattattct tatgtcacga caaaaaacat taacgcagag aaattattag
      121 cgccacagtc agtttgtgaa aatgtgaaga tattcagaat ttttattcag tcatgataca
      181 ggcatcctcc gggatgcctg ttgtttttgt gcgtaacagt tatcacagta aagggtgaga
      241 caatgggcaa aggtggcggc aaggcgcaca cgccggttga ggcaaaggac aatcttaagt
      301 ccacgcagat gatgagcgtg attgatgc
//
LOCUS       DAZFJF010000531          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFJF010000531 DAZFJF010000000
VERSION     DAZFJF010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.534"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /locus_tag="ACHKAU_005682"
     CDS             <1..231
                     /locus_tag="ACHKAU_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534473.1"
                     /translation="ISADIILTRTPHITEFSSDSITAQIKKNGINIQTGRMVSQKHLI
                     ITHDQEGKHLTTWYQQISLRVHSYKKHTEEVR"
BASE COUNT          133 a           76 c           44 g           73 t
ORIGIN      
        1 atctcagcag atattatcct gaccaggaca cctcacatta ctgaattctc ttccgacagt
       61 atcactgcac aaataaaaaa gaacgggata aatattcaga ccggaagaat ggtgtcacaa
      121 aagcatttaa tcattacgca tgaccaggaa gggaaacact taaccacatg gtatcaacag
      181 atatcattac gtgtgcactc ttataaaaaa cacacagaag aagtaagata attataaacg
      241 taaatttaat atctcccact gacatctccc ccacaataca ctaaaaaata ctcacagcag
      301 taaacacaga cacacctgat aatcat
//
LOCUS       DAZFJF010000532          324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFJF010000532 DAZFJF010000000
VERSION     DAZFJF010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.535"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..146)
                     /locus_tag="ACHKAU_005683"
     CDS             complement(18..146)
                     /locus_tag="ACHKAU_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO5534474.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            complement(103..>324)
                     /locus_tag="ACHKAU_005684"
     CDS             complement(103..>324)
                     /locus_tag="ACHKAU_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5534475.1"
                     /translation="RAAARWGEVMQLVETDKERHHITMRRLECSRKAQRAPEPPDNFG
                     DLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
BASE COUNT           63 a          101 c           68 g           92 t
ORIGIN      
        1 tttgtttcag atagtaacta tgcctgaccg tgacgaaaac ccggtaatgc gccataccgt
       61 ttcaactggt gcaaaaaaag ccggatttct ccggctgttt gattagctgt cctggtaatt
      121 gcgccatatt tcatcaccag caccatctat acccatttcg gcataagtgc gatcgactgc
      181 ctttttcagg tctccgaaat tatccggcgg ctccggtgcc ctctgtgcct tccttgaaca
      241 ttccagccgt cgcatcgtga tgtgatgccg ttccttgtct gtctccacca gctgcatgac
      301 ttcaccccat cgcgccgccg ccct
//
LOCUS       DAZFJF010000533          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFJF010000533 DAZFJF010000000
VERSION     DAZFJF010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.536"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>323)
                     /locus_tag="ACHKAU_005685"
     CDS             complement(<1..>323)
                     /locus_tag="ACHKAU_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534476.1"
                     /translation="QIVLGAAAIVGSFFTAGATMALWGSALAAGGFSATTMLFSLGAS
                     MILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRI
                     SQDIS"
BASE COUNT           68 a          102 c           88 g           65 t
ORIGIN      
        1 gctgatgtcc tgggagattc gccgggagcc aaccagcatt tcaccgtaag gcaccggcat
       61 cgggttcccc tgggcaatca tgttgtccag tgacgaaaaa tacgtgttct gtttaccgtt
      121 atccgtactt ttgtactccg gcgtctttgc cttcggggcc agcatctggg ccacaccgcc
      181 cagtatcatg ctggccccca gtgaaaacag catcgtggtg gcagaaaaac cgccggctgc
      241 cagggctgaa ccccataacg ccatcgttgc gccggcggtg aagaaagagc ccacgatggc
      301 tgccgccccc agcacaatct gca
//
LOCUS       DAZFJF010000534          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFJF010000534 DAZFJF010000000
VERSION     DAZFJF010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.537"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>323)
                     /locus_tag="ACHKAU_005686"
     CDS             complement(<1..>323)
                     /locus_tag="ACHKAU_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5534477.1"
                     /translation="ALPWWLVCRGDIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLA
                     GTEMPDFHREDDWWRNGQNLYLDNMAVTGFYRVPLSSAQAGDILLCCFGASVPNHAAI
                     YCGNG"
BASE COUNT           79 a          101 c           88 g           55 t
ORIGIN      
        1 tcaccgttgc cgcagtaaat ggcggcatgg ttcggtaccg aagcaccaaa gcagcacagc
       61 agaatatcgc ccgcctgtgc agaggacagg ggcacccggt aaaagccggt gaccgccata
      121 ttgtccaggt aaaggttctg accgttgcgc caccagtcat cctcgcgatg aaaatccggc
      181 atttcagtcc ccgccagatg ataagcatcc cggaacagcg tgtaacagtc cgtcaccccg
      241 tgctcaaagc gccgtcctgt cagatgtggc acacagcgga atttgtgaat gtcaccccgg
      301 cagaccagcc accagggcag tgc
//
LOCUS       DAZFJF010000535          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFJF010000535 DAZFJF010000000
VERSION     DAZFJF010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.538"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>322
                     /locus_tag="ACHKAU_005687"
     CDS             <1..>322
                     /locus_tag="ACHKAU_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5534478.1"
                     /translation="AGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYK
                     DLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQ
                     CVGGS"
BASE COUNT           68 a           72 c          108 g           74 t
ORIGIN      
        1 atgcgggcat tctgctggtt ccgtgctgtc gtggtggttc agcgttcacc accggagccg
       61 atggcacata cagtgacgcg agtggtgcct cggagaattc aacccgctgg ggtgtggaca
      121 agccgctgta taaggacctt atcggtcgaa caaaagcagc actgaagaag aatccgaaaa
      181 atgtgctgtt tgccgtggtg tggatgcagg gggaatttga ttttggcggt acgccggcaa
      241 atcacgcagc acagtttggt gcgctggttg ataaattccg tgcagacctg gcggatatgg
      301 caggtcagtg cgtcggtggc tc
//
LOCUS       DAZFJF010000536          319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFJF010000536 DAZFJF010000000
VERSION     DAZFJF010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.539"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(226..>319)
                     /locus_tag="ACHKAU_005688"
     CDS             complement(226..>319)
                     /locus_tag="ACHKAU_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO5534479.1"
                     /translation="YRARETDGSASQTFDSGSQGQIQGKITGMP"
BASE COUNT           69 a           95 c           61 g           94 t
ORIGIN      
        1 aattatttag tacaaaaagt gaacttttga tgatctttcc ctaatctcac ataggatcag
       61 atctcgtctg atcgttcccc ctgcgctcac taccatggct ctttaccaca gcagccgggg
      121 ttgtttggga ccgacttccg acagcagatc ccggtgggcc tgccaccatc tcttcacaag
      181 cttatgctta ttacatgcaa aacgtcccgt tttgcatctt tgtgcctacg gcatccctgt
      241 gatctttccc tgaatctgcc cctgagaacc tgaatcaaac gtctgactcg cagaaccatc
      301 tgtttctcgc gctctgtag
//
LOCUS       DAZFJF010000537          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFJF010000537 DAZFJF010000000
VERSION     DAZFJF010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.denovo.540"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>314
                     /locus_tag="ACHKAU_005689"
     CDS             <1..>314
                     /locus_tag="ACHKAU_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO5534480.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZFJF010000538        11419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.guided.130, whole genome shotgun sequence.
ACCESSION   DAZFJF010000538 DAZFJF010000000
VERSION     DAZFJF010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.guided.130"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..208)
                     /locus_tag="ACHKAU_005690"
     CDS             complement(<1..208)
                     /locus_tag="ACHKAU_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO5534481.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLW"
     gene            complement(205..519)
                     /gene="yjeN"
                     /locus_tag="ACHKAU_005691"
     CDS             complement(205..519)
                     /gene="yjeN"
                     /locus_tag="ACHKAU_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO5534482.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(571..2073)
                     /gene="yjeM"
                     /locus_tag="ACHKAU_005692"
     CDS             complement(571..2073)
                     /gene="yjeM"
                     /locus_tag="ACHKAU_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5534483.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(2292..3269)
                     /gene="epmA"
                     /locus_tag="ACHKAU_005693"
     CDS             complement(2292..3269)
                     /gene="epmA"
                     /locus_tag="ACHKAU_005693"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5534484.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            3594..5402
                     /gene="frdA"
                     /locus_tag="ACHKAU_005694"
     CDS             3594..5402
                     /gene="frdA"
                     /locus_tag="ACHKAU_005694"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5534485.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATELAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            5395..6129
                     /gene="frdB"
                     /locus_tag="ACHKAU_005695"
     CDS             5395..6129
                     /gene="frdB"
                     /locus_tag="ACHKAU_005695"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5534486.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            6140..6535
                     /gene="frdC"
                     /locus_tag="ACHKAU_005696"
     CDS             6140..6535
                     /gene="frdC"
                     /locus_tag="ACHKAU_005696"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5534487.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            6546..6905
                     /gene="frdD"
                     /locus_tag="ACHKAU_005697"
     CDS             6546..6905
                     /gene="frdD"
                     /locus_tag="ACHKAU_005697"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5534488.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            6968..8101
                     /locus_tag="ACHKAU_005698"
     CDS             6968..8101
                     /locus_tag="ACHKAU_005698"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5534489.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            8190..8723
                     /gene="blc"
                     /locus_tag="ACHKAU_005699"
     CDS             8190..8723
                     /gene="blc"
                     /locus_tag="ACHKAU_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5534490.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(8720..9037)
                     /gene="sugE"
                     /locus_tag="ACHKAU_005700"
     CDS             complement(8720..9037)
                     /gene="sugE"
                     /locus_tag="ACHKAU_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5534491.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(9219..9365)
                     /gene="ecnB"
                     /locus_tag="ACHKAU_005701"
     CDS             complement(9219..9365)
                     /gene="ecnB"
                     /locus_tag="ACHKAU_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5534492.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(9476..9601)
                     /gene="ecnA"
                     /locus_tag="ACHKAU_005702"
     CDS             complement(9476..9601)
                     /gene="ecnA"
                     /locus_tag="ACHKAU_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5534493.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(9653..10219)
                     /gene="efp"
                     /locus_tag="ACHKAU_005703"
     CDS             complement(9653..10219)
                     /gene="efp"
                     /locus_tag="ACHKAU_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5534494.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            10261..11289
                     /gene="epmB"
                     /locus_tag="ACHKAU_005704"
     CDS             10261..11289
                     /gene="epmB"
                     /locus_tag="ACHKAU_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5534495.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2969 a         2973 c         2860 g         2617 t
ORIGIN      
        1 accagagaaa agggaaaaag agcggtaacg tcattatcac accaacatca cgggtatcgt
       61 cttcttcgag atacatcaac acatcgcaga cgttgttaat cccgcgtcca tcaatcatcc
      121 actctttgtc cagcgcggaa gtgatggtta tccagaggaa cgccacaata aaccagatgc
      181 agcataagac aaacatgcgc gcactcataa ggtaaccggt cgcttaattt cgctatagcg
      241 catacgaaac aaatcgccgt atcccactaa ttccccgcga aaaaccagac atcggataag
      301 ataagcgcaa taaatatccg gcaggtttcc ccaccgttta ccatcgcgat ctttgagggt
      361 gttaatggtg ctacccacca ccatcgccac tttacgggtg tggcaggcat cgacaaaaga
      421 gagaatcgtc tgctctattt ccgctacatc cccggcagaa aattgcagcg ccctgatttc
      481 atcttcggtt attgccgggt cccggctggt gtcatccata acatttcctg tctttacgcc
      541 ctatcgtcag gtagacgata gggcatcggg ttattccacc gcccactgcg gattttttgc
      601 cactctgctg caatagttct ggtaaatcgc catcgctaac agcgagaaga agacagggcc
      661 gccaatcatc cacaatgtgc tgtcccagtc tccggcttca accacaggtt gaatgatggt
      721 gaagacgttc gcaaatgtca ccaccagtac gacaaccact gttgcaatca ttgccgacat
      781 atgcgttttg aaaatcacaa atggtctgtc gagatcctga cgtgctttaa aaaatgggaa
      841 agccagcgcg aggaacaggt aaggaagcgt catagacacg ttcgccatca gcgtcagctt
      901 gttaaagaac gccgatgcgg taccgccacc aaacgaaacc agcaggatga agacagtaac
      961 caacccgcac tgcatccaca tggcgataga cggcatcccc atcgcattca ggcgcgtcat
     1021 cggttccggc cacaacgctt tcggcgtccc ctggatgatg gctttcagcg gtgaatagca
     1081 aagcgtaaag aacgcaccgg tataagcgag gaacatcgaa agtccggtaa tacgcgcaaa
     1141 ccatacgccc agcgacaatg acgcttcagg tgacaaatgc agtgcgttac ccagcgtcac
     1201 cccgaggctc ttcatcagca cataggtaat attgccaagg ttaacggaac cattacttaa
     1261 tacctgctgc cagtttgtgc tgacgcccca taaaaatatt gccagcgaat aaccgattga
     1321 aataacaata gcggcaaaaa caataccttt ggcaaagttc ttttctggat tttccgtttt
     1381 atcgaccaga ccaccaaccg cttcaattcc gccataggca aaaatagcaa ataccacaaa
     1441 cgatagcatt gccagaccgg actgataacc tgggttcggt gatgcaagga aattaatatc
     1501 ctgcgcgaaa tgcccaccat ttaataacaa aatagtaatg cttactaaca gcaatactaa
     1561 attcagacac attactgcaa taccgcccac cgcagtaatg cgggcaattt tattaatccc
     1621 ctttgaagca acgacggtga ccagaatcat ccatgccact gccagcagac caaccacctg
     1681 cgtaggctcc agtccagcaa tacgccagtg ctgggtcatg tcgctaccat agaggaatgt
     1741 tgagaacggt acccaaactt tcgcggaggt actcaccatc cagatgatat aagaggaaaa
     1801 ccacataaac gtaccaatga aggcaaaacg tggtccgaca ctattattca tccaggaata
     1861 gataccgcct tcttctttgc gataagcagc tcccatttca gccatcatta aggcgaatgg
     1921 aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac ccattaagta
     1981 ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca atatgagtcc
     2041 tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt cagcgcgtgt
     2101 ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta aaaaccaata
     2161 cagaattgat acctggatga tatcaaatat ccagtcttaa attgtggcct ttaacggaaa
     2221 aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt taattcagta
     2281 atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag tgtctccgcg
     2341 cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca gtcaggcata
     2401 ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa accgcgcgcc
     2461 gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc cgtcaattca
     2521 tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc agcgacccga
     2581 tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg gtacacaaag
     2641 gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa ttgtagcagc
     2701 gtgtcgcggt cttcttcggt atcagcaaca ttgctcaaat ccagtttcgc tgcgacttcc
     2761 cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa gaaagcttgt
     2821 tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag atcgtccacc
     2881 tcgttcatca accggtacat atcatagtgc ggtcgatacc actccagcat agtgaactca
     2941 gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg gaataccggc
     3001 ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat taaccagaga
     3061 ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac caaatgaata
     3121 tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc acgatcggca
     3181 aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg aatggatgcg
     3241 ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga caagggcacg
     3301 aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac agcggtgggc
     3361 agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc ttcagtgata
     3421 atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact tatccatcag
     3481 actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg taactttcag
     3541 gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa
     3601 cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt gctgcaattg
     3661 ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc
     3721 gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat catgacagct
     3781 tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag caggatgtcg
     3841 tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg tgggggtgcc
     3901 catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg
     3961 agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg ctgttccaga
     4021 cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg gatattctgg
     4081 ttgatgatgg tcatgttcgc ggcgtggtag caatgaacat gatggaaggc acgctggtgc
     4141 agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt tatcgttaca
     4201 acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc cacggcgttc
     4261 cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt tccggtatcc
     4321 tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt
     4381 atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa aacaaatata
     4441 tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg cgtaaaggca
     4501 acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cctgcgtcac ctcggcgaga
     4561 aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg
     4621 atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg ggcggtatcg
     4681 aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt gaatgttcct
     4741 ctgttggtct gcacggtgca aaccgtctgg gttctaactc cctggcggaa ctggtggtct
     4801 tcggccgtct ggccggtgaa caagcgacag agcttgcagc aactgccggt aatggcaacg
     4861 aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat ctggttaacc
     4921 aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca atggaagaag
     4981 gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag ctggcagagc
     5041 tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc
     5101 tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg gcgcactccg
     5161 caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc
     5221 gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat ggcacgactc
     5281 gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc gtttacggtg
     5341 gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag gcgaatggct
     5401 gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg
     5461 catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc
     5521 tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt
     5581 tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg
     5641 cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat
     5701 ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc
     5761 ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag
     5821 tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt
     5881 ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa
     5941 gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggcgta
     6001 tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct
     6061 gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa
     6121 ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc
     6181 cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt
     6241 tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga aaaatggccc
     6301 ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct
     6361 gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg caccgaaagc
     6421 ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg
     6481 ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc
     6541 ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg
     6601 gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc
     6661 tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg
     6721 cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg
     6781 gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct gcgggcaaat
     6841 gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt atcgttacaa
     6901 tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc
     6961 agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt gctccacatt
     7021 tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca
     7081 acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac
     7141 ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt
     7201 aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga
     7261 aatcaagtta agcgatccca caacaaaata ctggcctgaa cttaccgcta aacagtggaa
     7321 tgggatcaca ctattacatc tcgcaaccta cactgctggc ggcctgccat tgcaggtgcc
     7381 ggatgaggtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg
     7441 ggcgccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc
     7501 tgtgaagccg tctggtttga gttttgagca ggcgatgcaa acacgtgtct tccagccact
     7561 caaactcaac catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt acgcctgggg
     7621 atatcgcgaa ggcaaggcag ttcatgtttc gccaggggcg ttagatgctg aaacttatgg
     7681 tgtgaagtcg accattgaag atatggcctg ctgggtacga agcaatatga atccccgtga
     7741 tatcaacgac aaaacacttc agcaagggat acaactggca caatctcgct actggcaaac
     7801 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa atcctgacag
     7861 catcattaac ggcagtggca ataaaattgc actggcagca caccccgtaa aagcgattac
     7921 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acaggggcaa ccggcggatt
     7981 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atagtgatgc tggctaacaa
     8041 aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg ctctacagta
     8101 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     8161 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     8221 cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc gtaacagtag
     8281 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     8341 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     8401 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     8461 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     8521 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     8581 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     8641 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     8701 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     8761 ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc acaatgccgg
     8821 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctactggta
     8881 acgatttcat cgcccaggca agtagcgcca tactgacaat catcgccgtc acggtaataa
     8941 cactcggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc cataccactt
     9001 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga gaattggggc
     9061 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     9121 ttttgccagg tggcagggga atttcaacta ttaccttagt gactatcgca gaaaaaacaa
     9181 acggcacgac atacctgtcg taccgttatt gcttattgct attgctgcgc tttcgttgct
     9241 gcgccagaaa tcgcgttacc gccatcagaa atgtcttcac caacgccacg cgtggtgttg
     9301 caggcagtta atactgttga aagcaccaga acagaaaaga tcgctgcaat tgtcttcttc
     9361 accataacgt cttcctttta ggtaatgtta tttatgtttg cctatagcaa attaataata
     9421 gacaacatcg ccaaaaatga cggaaatctg atgatttttc ggaagagaag aaaaattagc
     9481 tggcagcgcg agagatggag ttgccgagat gtttgatgtc ttcgccgaaa ccgcgagcgg
     9541 tgttacagcc cgtgagcagc gtgctggcaa gcaaaacaag aacgataagg cgtttcatca
     9601 tctctgacct gaataagtga tggtgcagcc tgcaggccgc accacaaccg cattacttca
     9661 cgcgagagac gtattcacca gagcgggtat ccactttgat gacttcgccg atttgtacga
     9721 acagcggaac cttaaccaca gcgccagtag acagggtagc cggtttgccg ccagtacctg
     9781 cggtatcacc tttcaggccc ggatcggtat caacgatttc cagttcaacg aagttcggcg
     9841 gagtaacgga gatcggctga ccattccaca gagttacgat acactctgcc tgatccagca
     9901 gccatttagc gttgtcgcca attgcttttg catcagcaga cagctgctcg aaagtttcgt
     9961 tgttcatgaa gtgccagaac tcaccgtcgt tgtacaggta agtcaggttc atatcgacaa
    10021 catcagcgcc ttcagcggaa tcagtagatt tgaaggtttt ttctacgcga gtaccggtca
    10081 gcagacgacg cagtttaacg cgagcaaatg cctggccttt acccggtttt acgaattcac
    10141 tcgcttcaac cgcgtaaggt tcgccgtcta acatgatttt aagaccagca cgaaaatcgt
    10201 tgctatagta cgttgccata aggccctctg aaatttgtta attggtagct aagccacaaa
    10261 atggcgcata ttgtaaccct aaatacccca tccagagaag attggttaac gcaacttgcc
    10321 gatgttgtga ccgatcctga tgaacttctg cgtcttttga atatagacgc ggacgaaaaa
    10381 ctgttggccg gacgcagcgc caaaaagctg tttgccctgc gtgtgccccg ctcatttatc
    10441 gatcgcatgg agaaaggcaa tccggacgat cctcttttgc gtcaggtact tacctcgcaa
    10501 gatgagtttg tcgtcgcgtc cggattctcc accgacccgc tggaagaaca gcacagcgta
    10561 gtgcctggtt tgttgcataa ataccacaac cgggcgcttt tgctggtcaa aggcggctgc
    10621 gcggtaaatt gccgctattg cttccgtcgc cacttcccct atgccgaaaa tcagggcaac
    10681 aagcgtaact ggcagactgc tcttgagtat gttgctgcgc atccggaact ggacgagatg
    10741 attttctccg gcggcgatcc gctgatggcg aaagatcacg agctggactg gttgctcaca
    10801 caactggaag ccatcccgca tatcaaacgt ctgcggattc acagccgtct gccgattgtg
    10861 atcccggcac gtatcaccga ggcgctggtt gaacgctttg cccgttctac gctgcaaatc
    10921 ttgctggtga atcacatcaa tcatgccaat gaggtagatg aaacgttccg tcaggcgatg
    10981 gctaaattgc gccgtgtcgg tgtcaccctg cttaaccaga gcgttctgtt acgtggtgtg
    11041 aacgataacg cacaaacgct ggcaaacctg agtaatgcgt tgttcgatgc cggcgtaatg
    11101 ccgtattacc tgcatgtgct cgataaagta cagggcgcgg cgcattttat ggtgagtgat
    11161 gacgaagcac ggcagattat gcgtgagttg ctgacactgg tgtcgggtta tctggtgccg
    11221 aaactggcgc gagaaatcgg cggcgaaccc agcaaaacgc cgctggatct ccagctacgc
    11281 cagcagtaac tgattaaaca gggaagttaa acattagcct gatgcgctac gcttatcagg
    11341 cctacattgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
    11401 tacgccgcat ccggcaaga
//
LOCUS       DAZFJF010000539         7830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.guided.181, whole genome shotgun sequence.
ACCESSION   DAZFJF010000539 DAZFJF010000000
VERSION     DAZFJF010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.guided.181"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..629
                     /locus_tag="ACHKAU_005705"
     CDS             <1..629
                     /locus_tag="ACHKAU_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO5534496.1"
                     /translation="RGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACIN
                     RQMIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSR
                     GDYSWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRD
                     KQLIFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            626..1240
                     /gene="prfH"
                     /locus_tag="ACHKAU_005706"
     CDS             626..1240
                     /gene="prfH"
                     /locus_tag="ACHKAU_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO5534497.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1297..2754)
                     /gene="pepD"
                     /locus_tag="ACHKAU_005707"
     CDS             complement(1297..2754)
                     /gene="pepD"
                     /locus_tag="ACHKAU_005707"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5534498.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3015..3473
                     /gene="gpt"
                     /locus_tag="ACHKAU_005708"
     CDS             3015..3473
                     /gene="gpt"
                     /locus_tag="ACHKAU_005708"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5534499.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3565..4809
                     /gene="frsA"
                     /locus_tag="ACHKAU_005709"
     CDS             3565..4809
                     /gene="frsA"
                     /locus_tag="ACHKAU_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5534500.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4867..5268
                     /gene="crl"
                     /locus_tag="ACHKAU_005710"
     CDS             4867..5268
                     /gene="crl"
                     /locus_tag="ACHKAU_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5534501.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5307..6362)
                     /gene="phoE"
                     /locus_tag="ACHKAU_005711"
     CDS             complement(5307..6362)
                     /gene="phoE"
                     /locus_tag="ACHKAU_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5534502.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6650..7753
                     /gene="proB"
                     /locus_tag="ACHKAU_005712"
     CDS             6650..7753
                     /gene="proB"
                     /locus_tag="ACHKAU_005712"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5534503.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7765..>7830
                     /locus_tag="ACHKAU_005713"
     CDS             7765..>7830
                     /locus_tag="ACHKAU_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5534504.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSR"
BASE COUNT         1896 a         1934 c         2049 g         1951 t
ORIGIN      
        1 cgcggggggt ggggcagtct attttacagc ggcatattgc ctcgttctcg catcatggtt
       61 tgcctcaagg cagtgacgac gcgctacggt atattgcgga acatgacgat gcgctggcgt
      121 ttgcgtgtat taatcgccag atgatcgctt tgcgcataat gcaacaagtt aaggccacgg
      181 gaagtccggt tctggatgtg gcgcataact ttgttagcgc gtgtcaaatc ggcgatcaac
      241 agggctggtt gcatcgtaaa ggtgccacgc cggatgacaa cggtctggtg attattcccg
      301 gttcacgtgg tgattactcc tggattgtac agcctgttgc gaacgaaaaa acgctccact
      361 cgctggcgca tggggcaggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag
      421 cgaaatacac ggcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg
      481 ataaacaact catctttgaa gaagcgccac aagcctataa atcggctgaa agcgtggtgc
      541 aatgtctggt gcaggccggt ttaattattc ctgtcgcgcg gctgcgtccg gtgctaacgc
      601 tcaaaaacag tggagggaaa aaaggatgat cttgctacaa ctctcctcag cgcaggggcc
      661 ggaagaatgt tgtctcgcag tgaaaaaggc gctggacagg ctaattaaag aagctgcccg
      721 acaagacgtc gcggtaacgg tgctggaaac agaaacgggc cactactctg acacgctgcg
      781 ttcggcgctg atttctctgg atggcgataa cgcatgggca ttaagcgaaa gctggtgtgg
      841 cactattcag tggatttgtc cgagtccgta tcggcctcat catgggcgca aaaactggtt
      901 tctgggcatt gggcgtttta ccgctgatga gcaggaacaa tcggatgcaa tccgttatga
      961 gacgttgcgt tcgtcggggc cgggcggtca acatgtcaat aaaaccgact cggcggtacg
     1021 cgccacgcat ttggcatccg gtattagcgt gaaggttcag tcagagcgta gtcagcatgc
     1081 taacaagcgg ctggcacgat tgctgattgc ctggaagctg gagcagcagc aacaggaaaa
     1141 tagcgcggtg ctgaaatcgc tgcggcgaat gttccatcac cagattgaac gtggcaaccc
     1201 gagacggaca tttacaggga tggcttttat cgaaggataa tgaaggatga aactgccgga
     1261 aggcgattaa acgccatccg gcagcaaatc aaataattac ttcgccggaa tttctttcag
     1321 cagttcagtc agcagcgtcc agtaatgacc tacgctttcg atgtgaactt gctcatccgg
     1381 agagtgtgga ccggtgatag ttggcccgat agaaaccatg tccatttccg gatacggttt
     1441 tttgaacaga ccacattcca ggcccgcgtg gataatctgg atgttcggtg ttttgttgaa
     1501 cagacgctga taggtttcac gtaccagatg catcaccgga gaattagcgt ccggctgcca
     1561 gccaggatat gcgcctttcg cttcggtttt cgcgccagct agtttaccca gcgaatccag
     1621 catgctcacc acgtagtctt taccgctgtc gatcagtgaa cggatcaggc agtgaatttc
     1681 tacgttattg tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt
     1741 tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt
     1801 atcgcgagat ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc
     1861 cagatttttc tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag
     1921 agatttcagg acgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg
     1981 gatggcgtta cgcagtgtgc cgccgttgaa atcgataagg cgcagatcca gttcttccgc
     2041 atgacccgcc aggaagcgca ccagtagttt gttggcatta cccaggccaa cgtggatttc
     2101 accgccggag tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc
     2161 tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc
     2221 catgtagatt tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca
     2281 gttgctctgt aagccgaacg caccgtccat accggcttct tcggtcatgg tcagcagcac
     2341 ttccagcggg ccgtgaacca cgttttcgtc agccagaacc gccagcgcag acgccatacc
     2401 aatgccgtta tctgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata
     2461 aggctggata ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat
     2521 atcgaggtgg gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttt
     2581 acgaatcagg atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca
     2641 accaacaatg tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat
     2701 tttggcaaaa atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag
     2761 tctccttgtc gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg
     2821 tcaacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     2881 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     2941 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     3001 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     3061 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     3121 gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
     3181 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     3241 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     3301 ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     3361 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg
     3421 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     3481 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca
     3541 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     3601 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
     3661 ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac
     3721 cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt
     3781 attgtgatga gcgatgctga acgtaccgac gatgatttat acgatactgt gattggctac
     3841 cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc
     3901 tgtgcggaag aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac
     3961 aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta
     4021 tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag
     4081 tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc
     4141 ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc
     4201 ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccatcg
     4261 gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc
     4321 ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc
     4381 cgtttcggcg ctaacgttgc cgtgcgtctg gcatatcttg aatcgccgcg tctgaaagcg
     4441 gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag
     4501 gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgat
     4561 gacgcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctggggcgt
     4621 cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag
     4681 gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac
     4741 ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaaacgc
     4801 ttgtgttaaa aatttgctaa attttgccaa tttggtaaaa cagttgcatc acaacaggag
     4861 atagcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca
     4921 ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg
     4981 gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg agttctgggg ctggtggatg
     5041 gagcttgaag cgcaggaatc ccgttttacc tacagttacc agtttggtct gttcgataaa
     5101 gcaggcgact ggaagagtgt tccggtaaaa gacactgaag tggttgaacg actggagcac
     5161 accctgcgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg
     5221 gaaccggcgg atgattttcg tgacgagccg gtgaagttaa cggcgtgagt gaaatgtgcc
     5281 ggatgcatca catccggcaa taatcattaa aactgatagg tcatgccaac cgcgacaata
     5341 tcatcattat taatattcaa tttgttatcg ctatccagtt ggttgatttt ataatcaaca
     5401 aacgctgaca tatttttgtt gaaataatat gtagccccga catcgatata attgaccaga
     5461 tcttcatcac cgataccttc aatatctttc cctttcgata agacataacc cagcgatgga
     5521 cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgtgt cttattggca
     5581 aagccgccag ttattggcgt cattttgcgt gtttcagaat aaaaagttgc cagataaaaa
     5641 ttattggcat cgtatttcag acctgtagcc catgcttctg cacgcttgcc tgtgccacgg
     5701 ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat aggccccact aatggcgaaa
     5761 tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc catcgccgtt ttgcttttta
     5821 acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc atcgataacg
     5881 ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt cataaagttg
     5941 tcggtctgcg ccgaggaatc gccaccaaat tccgggaaca tatcggtcca ggcttccacg
     6001 tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt atatttcaac
     6061 ccggcaaaag cgagacgcgt tttttgctgt gcggtatcac tctccgcttt atttccggca
     6121 aactccgctt cccaccgacc ataaccagtc agttgatcgt taatttgtgt ttcaccttta
     6181 aaaccaaaac ggatataact ctggtcgcca tctttactgg cgttatcact catataatgc
     6241 atggctttaa ctttgccata gacatccagt ttattaccgt ctttattata tatttctgca
     6301 gcctgtacag atgcagatgc cacaatgccc atcaccacta atgccagagt gctctttttc
     6361 attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagccc cgttgaaaac
     6421 aggaaagttt ttaacctgag attgttaaag atatattaca gattaataat attcttaaaa
     6481 tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctagg cttgctgatc
     6541 ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct
     6601 aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag
     6661 ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa
     6721 ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg
     6781 gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga
     6841 actgccagcg actatcgcct cgaaacaact gctggcagcg gtagggcaga gtcgactgat
     6901 tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac
     6961 ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt
     7021 gctcgataac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat
     7081 taaagtcggc gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa
     7141 actgttgctg ctgaccgatc aaaaaggttt gtataccgct gacccgcgca gcaatccgca
     7201 ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga
     7261 cagcgtttca ggcctcggaa ctggcggcat gagtaccaaa ttgcaggctg ctgacgtggc
     7321 ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga
     7381 tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa
     7441 ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc
     7501 aactgccgcc attcttgaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac
     7561 tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc
     7621 ccacggcgtc agtcgttaca acagcgatgc attacgccgt attgccgggc accactcgca
     7681 agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat
     7741 gattacccgt taaggagcag gctgatgctg gaacaaatgg gcattgccgc gaagcaagcc
     7801 tcgtataaat tagcgcaact ctccagccgc
//
LOCUS       DAZFJF010000540         1737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110H11661
            SAMN43267674-rid23066243.guided.341, whole genome shotgun sequence.
ACCESSION   DAZFJF010000540 DAZFJF010000000
VERSION     DAZFJF010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267674
            Sequence Read Archive: SRR30307504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350822.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:42:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 96
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 107 of 254
            Pseudo Genes (incomplete)         :: 139 of 254
            Pseudo Genes (internal stop)      :: 61 of 254
            Pseudo Genes (multiple problems)  :: 44 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267674-rid23066243.guided.341"
                     /serotype="O 26:H11"
                     /isolate="2110H11661"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..1210
                     /gene="stxA1a"
                     /locus_tag="ACHKAU_005714"
     CDS             263..1210
                     /gene="stxA1a"
                     /locus_tag="ACHKAU_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO5534505.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1220..1489
                     /gene="stxB1a"
                     /locus_tag="ACHKAU_005715"
     CDS             1220..1489
                     /gene="stxB1a"
                     /locus_tag="ACHKAU_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5534506.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          463 a          304 c          428 g          542 t
ORIGIN      
        1 gagtttgcca gcctccccca gtggctggct tttttatgtc cgtaacatcc tgtgtatcaa
       61 taaatgttgt tatctacgta cgtcaagtag tcgcatgaga tctgaccaga tatgttaagg
      121 ttgcagctct ctttgaatat gattatcatt ttcattacgt tattgttacg tttatccggt
      181 gcgccgtaaa acgccgtcct tcagggcgtg gaggatgtca agaatatagt tatcgtatgg
      241 tgctcaagga gtattgtgta atatgaaaat aattattttt agagtgctaa cttttttctt
      301 tgttatcttt tcagttaatg tggtggcgaa ggaatttacc ttagacttct cgactgcaaa
      361 gacgtatgta gattcgctga atgtcattcg ctctgcaata ggtactccat tacagactat
      421 ttcatcagga ggtacgtctt tactgatgat tgatagtggc acaggggata atttgtttgc
      481 agttgatgtc agagggatag atccagagga agggcggttt aataatctac ggcttattgt
      541 tgaacgaaat aatttatatg tgacaggatt tgttaacagg acaaataatg ttttttatcg
      601 ctttgctgat ttttcacatg ttacctttcc aggtacaaca gcggttacat tgtctggtga
      661 cagtagctat accacgttac agcgtgttgc agggatcagt cgtacgggga tgcagataaa
      721 tcgccattcg ttgactactt cttatctgga tttaatgtcg catagtggaa cctcactgac
      781 gcagtctgtg gcaagagcga tgttacggtt tgttactgtg acagctgaag ctttacgttt
      841 tcggcaaata cagaggggat ttcgtacaac actggatgat ctcagtgggc gttcttatgt
      901 aatgactgct gaagatgttg atcttacatt gaactgggga aggttgagta gcgtcctgcc
      961 tgactatcat ggacaagact ctgttcgtgt aggaagaatt tcttttggaa gcattaatgc
     1021 aattctggga agcgtggcat taatactgaa ttgtcatcat catgcatcgc gagttgccag
     1081 aatggcatct gatgagtttc cttctatgtg tccggcagat ggaagagtcc gtgggattac
     1141 gcacaataaa atattgtggg attcatccac tctgggggca attctgatgc gcagaactat
     1201 tagcagttga gggggtaaaa tgaaaaaaac attattaata gctgcatcgc tttcattttt
     1261 ttcagcaagt gcgctggcga cgcctgattg tgtaactgga aaggtggagt atacaaaata
     1321 taatgatgac gataccttta cagttaaagt gggtgataaa gaattattta ccaacagatg
     1381 gaatcttcag tctcttcttc tcagtgcgca aattacgggg atgactgtaa ccattaaaac
     1441 taatgcctgt cataatggag ggggattcag cgaagttatt tttcgttgac tcagaatagc
     1501 tcagtgaaaa tagcaggcgg agattcataa atgttaaata catctcaatt cagtcagttg
     1561 ttgccggtct gataatagat gtgttagaaa atttctgcat ggtgaatccc cctgtgcgga
     1621 ggggcgactg gtgaacggta tgatctcttt gatgatcgta agcgagaata cgcgggtttg
     1681 gtggcaccag gccgaactca ccgggaggca cccggcatca tgctgtatac agagatt
//
